U.S. patent application number 15/840834 was filed with the patent office on 2018-05-10 for sers system employing nanoparticle cluster arrays with multiscale signal enhancement.
The applicant listed for this patent is Trustees of Boston University. Invention is credited to Luca Dal Negro, Bjoern Markus Reinhard.
Application Number | 20180128947 15/840834 |
Document ID | / |
Family ID | 42316859 |
Filed Date | 2018-05-10 |
United States Patent
Application |
20180128947 |
Kind Code |
A1 |
Reinhard; Bjoern Markus ; et
al. |
May 10, 2018 |
SERS SYSTEM EMPLOYING NANOPARTICLE CLUSTER ARRAYS WITH MULTISCALE
SIGNAL ENHANCEMENT
Abstract
Defined nanoparticle cluster arrays (NCAs) with dimensions up to
25.4 .mu.m square are fabricated on a 10 nm gold film using
template guided self-assembly. Structural parameters are precisely
controlled, allowing systematic variation of the number of
nanoparticles in the clusters (n) and edge to edge separation ( )
between 1<n<20 and 50 nm.ltoreq. .ltoreq.1000 nm,
respectively. Rayleigh scattering spectra and surface enhanced
Raman scattering (SERS) signal intensities as functions of n and
reveal direct near-field coupling between the particles within
individual clusters, whose strength increases with cluster size (n)
until it saturates at around n=4. Strong near-field interactions
between clusters significantly affects the SERS signal enhancement
for edge-to-edge separations <200 nm. The NCAs support
multiscale signal enhancement from simultaneous inter- and
intra-cluster coupling and |E|-field enhancement. Applications
include SERS-based spectral identification of bacteria.
Inventors: |
Reinhard; Bjoern Markus;
(Boston, MA) ; Dal Negro; Luca; (Brookline,
MA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Trustees of Boston University |
Boston |
MA |
US |
|
|
Family ID: |
42316859 |
Appl. No.: |
15/840834 |
Filed: |
December 13, 2017 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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14657279 |
Mar 13, 2015 |
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15840834 |
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13142939 |
Jun 30, 2011 |
9013689 |
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PCT/US2010/020640 |
Jan 11, 2010 |
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14657279 |
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61143670 |
Jan 9, 2009 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
G01N 21/01 20130101;
G01N 21/658 20130101; G01N 2201/02 20130101; Y10T 428/12104
20150115; Y10T 428/24909 20150115; C03C 17/40 20130101; B82Y 30/00
20130101; C03C 2217/255 20130101; C03C 2217/425 20130101; G02B
5/008 20130101 |
International
Class: |
G02B 5/00 20060101
G02B005/00; B82Y 30/00 20060101 B82Y030/00; C03C 17/40 20060101
C03C017/40; G01N 21/01 20060101 G01N021/01; G01N 21/65 20060101
G01N021/65 |
Goverment Interests
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
[0001] This invention was made with Government Support under
Contract No. W911NF-06-2-0040 awarded by the Army Research Office.
The Government has certain rights in the invention.
Claims
1. A surface-enhanced Raman spectroscopy (SERS) system, comprising:
a SERS substrate having a nanoparticle cluster array on a surface
of a planar substrate layer, the nanoparticle cluster array
including an array of clusters of metal nanoparticles characterized
by a cluster size n and a cluster separation .LAMBDA., n being a
nominal number less than 10 of tightly-packed nanoparticles in each
cluster determined by a nominal size of the nanoparticles and a
deterministic binding site width D, and .LAMBDA. being a
deterministic distance less than 200 nm between adjacent clusters,
n and .LAMBDA. together defining a multiscale enhancement of a
resonant Raman optical signal induced in the SERS substrate upon
excitation with an incident optical signal of a corresponding
wavelength; an optical source configured and operative to generate
the incident optical signal and direct it at the SERS substrate,
causing the SERS substrate to emit a multi-scale enhanced resonant
Raman optical signal; and an optical detector configured and
operative to receive the multi-scale enhanced resonant Raman
optical signal and generate a corresponding detector output signal
indicative of amplitude of the multi-scale enhanced resonant Raman
optical signal.
2. The SERS system of claim 1, wherein the multi-scale enhancement
of the resonant Raman optical signal arises from plasmon coupling
on two distinct length scales, a first length scale being an
inter-particle length scale within the clusters and providing a
first stage of enhancement, a second length scale being an
inter-cluster length scale between the clusters and providing a
further increase of enhanced field.
3. The SERS system of claim 1, wherein the wavelength of the
incident optical signal is matched with an absorption band of the
SERS substrate to maximize enhancement of the resonant Raman
optical signal.
4. The SERS system of claim 3, wherein the wavelength of the
incident optical signal is lower than a resonance wavelength of a
reference array of gold nanoparticles of a predetermined size, and
the metal nanoparticles have a size and material providing
energetically lower particle resonances matched with the wavelength
of the incident optical signal.
5. The SERS system of claim 1, wherein the incident optical signal
from the optical source is non-polarized, wide-spectrum light.
6. The SERS system of claim 1, wherein the optical detector is an
imaging detector responding to a two-dimensional pattern of the
multi-scale enhanced resonant Raman optical signal from the SERS
substrate.
7. The SERS system of claim 6, wherein the imaging detector is an
imaging spectrometer providing spectral analysis of the multi-scale
enhanced resonant Raman optical signal from the SERS substrate.
8. The SERS system of claim 7, wherein the imaging spectrometer
performs background correction of the multi-scale enhanced resonant
Raman optical signal by subtracting a scattering signal from an
equal-size, non-patterned adjacent area of the SERS substrate.
9. The SERS system of claim 8, wherein the imaging spectrometer
performs additional correction by normalizing with a scattering
spectrum of an ideal scatterer on top of the SERS substrate.
10. The SERS system of claim 1, wherein the SERS substrate includes
a noble metal film on which the nanoparticle clusters are disposed,
and an optical response of the nanoparticles on the noble metal
film is determined by local plasmons of the particles, interactions
between the plasmons through space, and coupling to delocalized
plasmon modes supported by the noble metal film.
11. The SERS system of claim 10, wherein the noble metal film is
periodically corrugated by the nanoparticle clusters to act as a
grating coupler enabling photons incident on the SERS substrate to
excite a propagating surface plasmon in the noble metal film, the
surface plasmon being Bragg-scattered from the nanoparticle cluster
arrays.
12. The SERS system of claim 10, wherein the multi-scale enhanced
resonant Raman optical signal has a spectrum including a first peak
attributable to a nanoparticle cluster resonance coupled via
propagating surface plasmons in the noble metal film, and a
separate smaller peak in a shorter-wavelength band.
13. The SERS system of claim 12, wherein a periodic two-dimensional
structure of nanoparticle clusters on the noble metal film acts as
a transmission grating for components of the incident optical
signal, the transmission grating inducing diffraction of the
incident optical signal according to a grating relationship among
center-to-center separation of nanoparticle clusters, a detection
angle, a diffraction order, and refractive index, such that all
wavelengths fulfilling the grating relationship are included in a
scattering spectrum.
14. The SERS system of claim 1, wherein the nanoparticle clusters
assume a rhombohedral geometry in which inter-particle distances
are minimized, resulting in maximal plasmon coupling.
15. The SERS system of claim 1, wherein the nanoparticle clusters
are three-dimensional to provide interactions between nanoparticles
along an out-of-place spatial axis, shifting plasmon resonance
further into the red than for two-dimensional clusters.
16. A method of performing surface-enhanced Raman spectroscopy on a
sample, comprising: placing the sample on a surface of a SERS
substrate to create a mounted sample, the SERS substrate having a
nanoparticle cluster array on a surface of a planar substrate
layer, the nanoparticle cluster array including an array of
clusters of metal nanoparticles characterized by a cluster size n
and a cluster separation .LAMBDA., n being a nominal number less
than 10 of tightly-packed nanoparticles in each cluster determined
by a nominal size of the nanoparticles and a deterministic binding
site width D, and .LAMBDA. being a deterministic distance less than
200 nm between adjacent clusters, n and .LAMBDA. together defining
a multiscale enhancement of a resonant Raman optical signal induced
in the SERS substrate upon excitation with an incident optical
signal of a corresponding wavelength; locating the mounted sample
at a location for imaging in a Raman microscope; and operating the
Raman microscope with the mounted sample at location for imaging to
capture a Raman spectrograph of the mounted sample.
Description
BACKGROUND
[0002] The intensities and frequencies of vibrational transitions
measured in Raman spectra provide unique chemical signatures of
molecular species, but the sensitivity of Raman spectroscopy
suffers from the relatively low cross-section of inelastically
scattered Raman photons. It has been demonstrated that the
magnitude of Raman cross-sections can be greatly enhanced when the
Raman-active molecules are placed on or near a roughened noble
metal surface. Since then a wide variety of substrates have been
found to enable surface enhanced Raman spectroscopy (SERS) such as
aggregated noble metal colloids, metal island films, metal film
over nanospheres, particles grafted on silanized glasses, regular
holes in thin noble metal films and regular nanoparticle
arrays.
[0003] In general, traditional SERS substrates can be divided into
two fundamental substrate classes: random and engineered
substrates. Random substrates like fractal nanoparticle
agglomerates can support localized dipole modes which lead to high
SERS signal enhancements. However, the resonance wavelength, the
precise locations of the spots of giant |E|-field enhancement--so
called hot-spots--and the reproducibility of their enhancement
factors are difficult to control in completely random structures.
Another disadvantage specific to fractal nanoparticle aggregates is
that their mass density, and therefore the hot-spot density,
decreases with increasing fractal size.
[0004] Challenging applications of SERS in single molecule
spectroscopy or whole organism fingerprinting would greatly benefit
from engineered SERS substrates with rational design criteria that
generate high SERS enhancement reproducibly at spatially defined
locations. Consequently, regular nanoparticle arrays and other
nanofabricated SERS substrates, whose characteristic structural
parameters can be accurately controlled, are attracting interest as
SERS substrates with reproducible, high enhancement factors "by
design". The SERS enhancement in noble metal nanoparticle arrays
depends on both the properties of the constitutive building blocks
(nanoparticles) as well as the characteristics of their
arrangement. In general two separate electromagnetic regimes govern
the collective response of periodic metal-nanoparticle arrays: near
and far-field coupling. When the particles are separated by short
distances up to approximately D=1/k.sub.0=.lamda..sub.0/2.pi. (with
k.sub.0 and .lamda..sub.0 being the free space wavenumber and
wavelength, respectively), strong quasi-static near-field
interactions dominate the response of the array. Consequently,
localized modes with strongly enhanced local fields are excited.
When the particles are separated by larger distances, far-field
diffractive coupling between the particles becomes dominant.
[0005] In the near-field coupling regime the field enhancement and
corresponding SERS intensity arising from periodic arrays of
nanoparticles sharply increases with decreasing inter-particle
separation. Both theoretical and experimental studies have shown
that regions of high |E|-field enhancement are located in the
junction between individual particles. The |E|-field enhancement in
these spatially confined hot spots can be orders of magnitude
larger than on the surface of individual particles. Due to the
rapid decay of the field strength with inter-particle separation
and the |E|.sup.4 scaling of the SERS signal, very short
inter-particle separations are vital in order to maximize the Raman
enhancement in the near-field coupling regime. Ideally, the analyte
molecules are placed in the junctions between nearly touching metal
surfaces.
SUMMARY
[0006] It remains challenging to create junction plasmons at
predefined locations and with nanometer accuracy in current
top-down fabrication methods such as electron beam (e-beam)
lithography. The spatial resolution of e-beam lithography is
limited by laterally scattered secondary electrons, which makes it
difficult to reproducibly fabricate arrays with inter-particle
separations of less than 10 nm. In order to overcome this
limitation, an alternative approach is demonstrated here that can
be used to engineer SERS substrates with nanoscale inter-particle
separations reliably. Template guided chemical self-assembly is
used to create nanoparticle cluster arrays (NCAs) of defined size
with nanoscale inter-particle separations at predefined pattern
locations. E-beam lithography is not used to directly generate
plasmonic structures but instead to define binding sites on which
chemically synthesized gold nanoparticles can assemble.
Consequently, "hot" inter-nanoparticle junctions at predefined
locations in a regular array can be created, enabling the
possibility to control and optimize both near- and far-field noble
metal nanoparticle interactions.
[0007] The description herein includes a systematic
characterization of the optical scattering spectra and the Raman
signal intensity enhancements of these NCAs as function of cluster
size n and cluster edge-to-edge separation , comparing their
performance with non-patterned colloidal gold films and periodic
two-dimensional nanodisc arrays, and an application for spectral
identification of bacterial pathogens. Rapid bacteria diagnostics
are vital for improving the treatment outcomes of serious
infections and ensuring the appropriate use of antibiotic
strategies. A SERS based approach for bacterial detection and
identification relies on signal amplification techniques (PCR), and
thus offers several potential advantages, such as speed, reduced
susceptibility to contamination problems, ease-of-use and mixture
resolution for rapid, specific and sensitive bacterial diagnostics.
The key requirement for the success of this methodology is the
production of SERS substrates with large and reproducible signal
enhancement. In this study, we demonstrate that NCAs provide
reproducible SERS signals from different bacteria species including
Escherichia coli, Bacillus cereus, and Staphylococcus aureus.
[0008] Overview of Methods
[0009] Nanofabrication of Particle Binding Sites.
[0010] A fabrication process is described which begins with
spin-coating 180 nm polymethyl methacrylate (PMMA) 950 photoresist
on top of Au-coated (10 nm Au film) glass slides. The substrates
are subsequently soft-baked at 180.degree. C. for 20 min. Periodic
patterns of nanowells are then written with a Zeiss SUPRA 40VP SEM
equipped with Raith beam blanker and a nanopattern generation
system (NPGS). After e-beam writing, the photoresist is developed
in methyl isobutyl ketone (MIBK). Periodic patterns of nanowells
with inter-well separations (edge to edge) of 50 nm, 100 nm, 150
nm, 200 nm, 400 nm, 600 nm, 800 nm, and 1000 nm are generated by
this procedure.
[0011] Template Guided Self-Assembly of Gold Nanoparticle
Clusters.
[0012] Commercial citrate-stabilized 40 nm Au particles in aqueous
solution are concentrated by a factor of 10 by centrifugation. 100
.mu.L of the concentrated gold sol is then incubated with 5 .mu.L
of a 10 mM thiol-EG.sub.7-propionat (EG=ethylene glycol) aqueous
solution overnight at room temperature. The particles are cleaned
by centrifugation and re-suspended in a 10 mM phosphate buffer
pH=8.6 containing 40 mM NaCl. The patterned gold substrates are
incubated with a 1 mM aqueous solution of
thiol-(CH2).sub.11EG.sub.7-Amine for 15 minutes and then washed
with water. The Au particles solution is added on the top surface
of the substrates and incubated for 1 h. The particle solution is
removed by washing with water. After the samples are dried, PMMA
liftoff is performed with 1-Methyl-2-pyrroldinone.
[0013] Dark-Field Scattering Characterization of Periodic Cluster
Arrays.
[0014] Scattering images of the particle cluster arrays were
recorded using an upright microscope (Olympus BX51 WI). The
nanoparticle arrays were immersed in index-matching oil
(n.sub.r=1.5) and illuminated with unpolarized white-light from a
100W tungsten halogen lamp using an oil dark-field condenser (NA
1.2-1.4) in transmission mode. The light scattered from the arrays
was collected with a 60.times. oil immersion objective (NA=0.65)
and imaged using a digital camera with an active area of
620.times.580 pixels. The microscope was also equipped with a 150
mm focal length imaging spectrometer (Acton Research, InSpectrum
150) and a back-illuminated CCD detector (Hamamatsu INS-122B) that
enabled the spectral analysis of the scattered light using a 150
lines/mm grating. The scattering spectra were background corrected
by subtraction of the scattering signal from an equal-size,
non-patterned adjacent area. The scattering spectra were
additionally corrected by the excitation profile of the white light
source by normalizing with the scattering spectrum of an ideal
white light scatterer on top of the gold film.
[0015] SERS Measurements.
[0016] A Renishaw Raman microscope (model RM-2000) capable of
.about.2.lamda., spatial resolution was used to observe the
scattering excited by a 785 nm diode laser. The frequency
calibration was set by reference to the 520 cm-1 silicon phonon
mode. Paramercaptoaniline (pMA) was used to characterize the field
enhancement on the cluster arrays. The saturated aqueous pMA
solution was kept on the substrate for 10 min before removing with
a flow of nitrogen gas. A 50.times. objective (numerical aperture
NA=0.78) was used for signal collection. SERS spectra were acquired
with incident laser powers in the 0.44 to 7.34 mW range and
acquisition times of 10-60 seconds.
[0017] Calculation of Approximate SERS Enhancement Factors for
Paramercaptoaniline (pMA).
[0018] SERS enhancement factors, G, were calculated following
standard procedures G is defined here by:
G=(ISubstrate/NSubstrate)*(NReference/IReference) where ISubstrate
is the Raman intensity of a monolayer of pMA on the SERS substrate
and IReference is the Raman signal due to a pMA crystal. NReference
and NSubstrate refer to the number of pMA molecules in a monolayer
on the SERS substrate and in the focal region of the crystal,
respectively. An aperture was used to confine the sample detection
area to .sigma.=2.5 .mu.m.times.25 .mu.m. NSubstrate was obtained
as the ratio of active nanoparticle cluster array area within the
detection area and the cross-section of the pMA molecule
(.sigma..sub.pMA=0.3 nm.sup.2). For NCAs the active area is
estimated by multiplying the number of clusters in the laser spot
with the product of the average number of particles in the clusters
and the surface area of one hemisphere of a 40 nm gold
nanoparticle. NReference was calculated assuming a confocal depth
of 14 .mu.m and a density of 1.06 g/mL for solid pMA (molecular
weight=125 g/mol). For non-patterned colloidal gold films the
surface-densities of 40 nm gold particles were obtained by counting
the number of particles in representative SEM images with defined
dimensions. Then NSubstrate was calculated using the same
assumptions as in the case of the NCAs. In the case of smooth
nanodisc arrays, NSubstrate was determined as the ratio of active
area (number of discs in the detection area multiplied by the
exposed disc surface area) and .sigma..sub.pMA.
[0019] Bacteria Growth and Sample Preparation.
[0020] Gram-negative bacteria Escherichia coli (ATCC #12435), and
Gram-positive bacteria Bacillus cereus (ATCC #14579) and
Staphylococcus aureus (ATCC #25904) were grown in 15-20 mL of LB
(Sigma) for .about.5 h at 37.degree. C. until they reached an
OD600=.about.0.6. About 4 mL of each culture solution was washed,
centrifuged and vortexed four times with Millipore water. Finally,
the pellet was suspended in 0.25 mL of water. About 1 .mu.L of the
bacteria suspension was placed on the cluster arrays, and after the
water had evaporated (.about.2 minutes), the samples were
transferred into the Raman microscope to record SERS spectra.
BRIEF DESCRIPTION OF THE DRAWINGS
[0021] The foregoing and other objects, features and advantages
will be apparent from the following description of particular
embodiments of the invention, as illustrated in the accompanying
drawings in which like reference characters refer to the same parts
throughout the different views. The drawings are not necessarily to
scale, emphasis instead being placed upon illustrating the
principles of various embodiments of the invention.
[0022] FIGS. 1 and 2 are a top view and side view respectively of a
substrate for use in SERS spectroscopy;
[0023] FIGS. 3 and 4 are magnified top and side views respectively
of the substrate of FIGS. 1 and 2;
[0024] FIGS. 5 and 6 are sets of scanning electron microscope (SEM)
images of a top surface of a SERS substrate;
[0025] FIG. 7 is a plot of normalized SERS scattering spectra for
various values of inter-cluster separation;
[0026] FIG. 8 is a plot of peak resonance wavelength as a function
of inter-cluster separation;
[0027] FIG. 9 is a plot of cluster size distribution as a function
of size of binding site;
[0028] FIG. 10 is a plot of peak wavelength as a function of
cluster size, along with an inset showing corresponding Rayleigh
scattering spectra;
[0029] FIG. 11 is a set of diagrams illustrating a method and
corresponding workpieces in fabricating a SERS substrate;
[0030] FIG. 12 is a diagram depicting binding of a gold
nanoparticle to a binding site;
[0031] FIG. 13 is a plot of SERS spectra of a monolayer of pMA for
various values of inter-cluster separation;
[0032] FIG. 14 is a plot of SERS enhancement factors for a
monolayer of pMA as a function of inter-cluster separation;
[0033] FIG. 15 is a plot of SERS intensity for a monolayer of pMA
as a function of binding site size;
[0034] FIG. 16 is a plot of SERS enhancement factor for a monolayer
of pMA as a function of cluster size;
[0035] FIG. 17 is a plot showing a comparison of SERS enhancement
factors for different types of SERS substrates;
[0036] FIG. 18 is a plot showing Rayleigh scattering spectra for
the different types of SERS substrates of FIG. 17;
[0037] FIG. 19 is a plot showing SERS spectra of three different
bacteria S. aureus, E. coli, and B. cereus;
[0038] FIG. 20 is a discriminant function analysis (DFA) plot
derived from the data of FIG. 19;
[0039] FIG. 21 is a plot of SERS spectra of a monolayer of pMA for
various values of laser excitation power; and
[0040] FIG. 22 is a plot of SERS signal strength as a function of
integration time.
DETAILED DESCRIPTION
[0041] FIGS. 1 and 2 show top and side views respectively of a
substrate 10 for use in SERS spectroscopy in the form of a glass
slide 12 having a patterned top surface 14 used as the active SERS
surface. FIG. 3 illustrates a close-up view of the top surface 14,
on which is formed an array of clusters 16 of gold nanoparticles.
FIG. 4 shows a set of adjacent clusters 16 in side view. The
schematic side view of FIG. 4 shows nanoparticles 18 of each
cluster 16. FIG. 4 also shows dimensions D and , with D being the
diameter of the binding sites at which each cluster 16 is located
and being an edge-to-edge separation of the clusters 16. The term
"cluster size" is used herein to refer to the number of
nanoparticles 18 per cluster 16. It will be appreciated that the
cluster size is a function of both the dimension D and the diameter
of a nanoparticle 18.
[0042] FIG. 5 shows scanning electron microscope (SEM) images of
sections of the fabricated cluster arrays 16 with a fixed
edge-to-edge separation ( =200 nm) but varying binding site
diameters (D=50 nm, 80 nm, 100 nm, 130 nm, 200 nm). The SEM images
show regular arrays of two-dimensional nanoparticle clusters with
varying cluster size. The images confirm that template guided
self-assembly approach (described below) leads to a spatially
confined particle clustering into discrete assemblies. FIG. 5 also
shows that the average cluster size (i.e. number of particles in
the clusters) can be conveniently controlled by varying the binding
site diameter D. For D=50, regular arrays of individual 40 nm gold
nanoparticles with a high degree of translational symmetry are
found. With increasing D, larger clusters are formed that exhibit
some geometry and size variability. Even under ideal assembly
conditions a distribution of particle numbers and cluster geometry
results from the natural size dispersion of the colloid used
(coefficient of variation <20%). The low number of particles in
the clusters makes the variation in the cluster size and shape most
striking for intermediate binding sizes of D=80 nm to 130 nm. In
contrast, the larger clusters appear more homogenous again.
[0043] Image (f) of FIG. 5 shows a particle cluster in the D=80 nm
NCA at higher magnification. As evident the particle clusters
contain holes, junctions, and crevices between a few
nanometer-spaced gold nanoparticles. These nanoparticle-gap
structures are known to support a strong |E|-field localization
which can induce a strong enhancement of the dipole moment of the
analyte molecules. The enhanced molecular dipole moments and the
amplification of the re-radiated Raman scattered light through the
noble metal nanostructures lead to a strong enhancement of the SERS
signal.
[0044] To test the field enhancing effect of inter-cluster
coupling, NCAs were made with varying cluster edge-to edge
separation . NCAs with =50, 100, 150, 200, 400, 600, 800, and 1000
nm and fixed binding site diameters between D=50-200 nm were made.
Of these, FIG. 6 shows examples with fixed binding site diameter D
of 200 nm and separations of 50 nm, 200 nm, and 1000 nm. It may be
difficult to decrease the edge-to-edge separation significantly
below =50 nm, especially for larger cluster sizes, because at very
short inter-particle separations individual nanoparticles clusters
tend to fuse and form continuous lines of nanoparticle clusters
across the pattern. Thus, the current template assisted
self-assembly approach can generate extended NCAs with a wide range
of inter-cluster separation and cluster sizes D, albeit with some
potential limitation for the minimum inter-cluster separation. The
following description presents a systematically analysis of the
optical response and the SERS enhancement as functions of and D to
elucidate an understanding the fundamental mechanisms governing the
multi-length scale electrodynamic interactions in NCAs.
[0045] Influence of the Cluster Edge-to-Edge Separation on the
Rayleigh Scattering Spectra of Nanocluster Particle Arrays.
[0046] The optical response of dielectrically coated gold
nanoparticles on a gold film is determined by the local plasmons of
the particles, their interactions through space (near-field and
far-field coupling) and by their coupling to delocalized plasmon
modes supported by the gold film. The localized modes in the
particles can couple with image modes in the gold film as well as
with plasmon modes in neighboring particles. In addition, a gold
surface which is periodically corrugated by gold nanoparticle
clusters can act as a grating coupler. In this case photons
incident on this surface can efficiently excite a propagating
surface plasmon in the gold film, which can be Bragg-scattered from
the regular cluster arrays.
[0047] Given these different electromagnetic interactions between
localized and delocalized plasmon modes in NCAs, it is challenging
to quantitatively predict the optical response of the nanoparticle
cluster arrays. As a first step towards an understanding of these
potential SERS substrates the spectral response of the NCAs can be
characterized as a function of the controllable template parameters
.LAMBDA. and D. To that end arrays may be fabricated with varying
cluster edge-to-edge separations =50-1000 nm but constant cluster
binding sizes D=200 nm. Since we kept the total number of binding
sites constant for all inter-cluster separations D, the total area
of the fabricated arrays varies from 25.4 .mu.m.times.25.4 .mu.m
for .ltoreq.1000 nm to 16 .mu.m.times.16 .mu.m for =50 nm. Due to
the small size of the arrays a spectral characterization of the
arrays using extinction spectroscopy would be challenging. Instead,
we decided to characterize the optical response of the NCAs using
Rayleigh scattering spectroscopy, which can be conveniently
performed in a darkfield microscope. With the help of a darkfield
condenser the excitation light can be injected into the specimen
plane at such a steep angle from the bottom that only scattered
light can reach the objective on top of the sample. The geometrical
constraints of the darkfield illumination allows effective
discrimination of the excitation light, and is therefore an ideal
technique for probing the plasmon resonances of nanostructures
which do not provide a strong extinction. Rayleigh scattering
spectroscopy is routinely used to investigate the optical
properties of a wide range of nanostructured plasmonic materials,
ranging from single noble metal nanoparticles over regular one- and
two-dimensional arrays of gold nanoparticles to deterministic
aperiodic arrays of gold nanoparticles.
[0048] FIG. 7 shows the normalized scattering spectra of the
fabricated NCAs with varying but fixed D=200 nm. The displayed
spectral intensities were not corrected by the different filling
fractions of the arrays, since we focus on the spectral shifts
originating from differences in the array parameters in this study.
All of the spectra in FIG. 7 show a broad peak around 700 nm, which
we assign to the nanoparticle cluster resonance coupled via
propagating surface plasmons in the gold support as has been
described elsewhere. In addition to this coupled nanoparticle
cluster resonance, NCAs with <200 nm exhibit a separate short
wavelength band which strongly red-shifts with increasing . In FIG.
8, the peak resonance wavelengths of these two bands, determined by
Gaussian best fits, are plotted as a function of . The maximum of
the short wavelength band shifts from .about.455 nm ( =50 nm) over
.about.545 nm ( =100 nm) to .about.613 nm (.LAMBDA.=150 nm). For
.LAMBDA..gtoreq.200 nm a separate second peak can no longer be
resolved. However, the spectrum of the NCA with .LAMBDA.=200 nm
(FIG. 7) is asymmetrically broadened on the high energy side,
indicating the possibility of additional spectral features.
[0049] Short wavelength bands next to the plasmon of the
nanostructures have been observed previously in extinction
measurements of regular arrays of smooth nanodiscs on gold and were
successfully ascribed to propagating surface plasmons or standing
waves due to Bragg scattering at the nanoparticle array on top of
the gold film. These models have, however, only limited
applicability in our scattering analysis of NCAs because the
investigated array geometries do not fulfill the grating coupling
conditions at our illumination angle (see below) in the
investigated wavelength range; in addition the correspondence
between calculated Bragg scattering resonances and the measured
high energy is approximate at best. Due to the experimental
geometry of the performed scattering experiments an alternative
explanation for the short wavelength bands arises from the
possibility that the periodic two-dimensional structure of gold
nanoparticle clusters on top of a transparent 10 nm thin gold film
acts as a transmission grating for some components of the incident
light. Diffraction of the wavelength .lamda. incident at angle
.phi..sub.inc on the oil immersed NCA is then described by the
grating formula:
L(sin .phi..sub.inc+sin .theta..sub.det)=(m/n.sub.r).lamda. (1)
where L is cluster center-to-center separation, .theta..sub.det is
the detection angle, m is the diffraction order, and n.sub.r is the
refractive index of glass and index matching oil (n.sub.r=1.5).
[0050] The incident angle .phi..sub.inc is determined by the
numerical aperture of the darkfield condenser. For a numerical
aperture of NA=1.2, .phi..sub.inc=53.degree.. The maximum detection
angle .theta..sub.det is given by the marginal ray as determined by
the objective numerical aperture. The numerical aperture of the
objective used in these studies (NA=0.65) results in a maximum
.theta..sub.det.ltoreq.25.7.degree.. All wavelengths that fulfill
equation (1) for 0.degree.<.theta..sub.det.ltoreq.25.7.degree.
can be folded into the scattering spectrum. Following this model
and considering the emission onset of the Tungsten lamp at
.about.400 nm we can assign peaks in the following spectral regions
to diffraction at the grating: .lamda.=400 nm-462 nm (.LAMBDA.=50
nm, L=250 nm); .lamda.=400 nm-555 nm (.LAMBDA.=100 nm, L=300);
.lamda.=419 nm-647 nm (.LAMBDA.=150 nm, L=350); .lamda.=479 nm-754
nm (.LAMBDA.=200 nm, L=400 nm). For larger .LAMBDA. the diffracted
wavelengths are shifted out of the detection range. The
experimentally observed short wavelength bands all fall in the
wavelength ranges predicted by equation (1). We conclude that the
simple transmission diffraction grating model suffices to explain
the observed dependence of the high energy band on .LAMBDA..
[0051] The frequency of the lower energy band, assigned to the
cluster plasmon resonance, appears to depend weakly on the cluster
edge-to-edge separation .LAMBDA. as shown in FIG. 8. This effect
is, however, much smaller than for the peak arising from
diffraction in the investigated .LAMBDA. range. The cluster
resonance peak (red line) slightly blue shifts from 720 nm for
.LAMBDA.=50 nm separations to 690 nm for .LAMBDA.=200 nm
structures. At larger edge-to-edge distances the cluster plasmon
resonance peak does not appear to shift further. This small
blue-shift with increasing inter-particle separation is attributed
to direct near-field interactions between the clusters of the
arrays on the gold substrate.
[0052] Influence of Cluster Size on the Rayleigh Scattering Spectra
of Nanoparticle Cluster Arrays.
[0053] As evidenced in FIG. 5, the average number of particles
within the individual building blocks of NCAs can be systematically
tuned by changing the diameter (D) of the binding site defined by
e-beam lithography. The cluster size distribution and average
cluster size n as a function of the binding size D for arrays with
fixed edge-to-edge separation .LAMBDA.=200 nm are quantitatively
described in FIG. 9. For D=50 nm individual 40 nm gold
nanoparticles are the predominant building block. However, the
number of particles on the binding sites grows with D, the site
diameter. The average cluster size n increases steadily from n=1.3
for D=50 nm to n=4.4 for D=120 nm and then jumps to n=19.1 for
D=200 nm (see FIG. 9).
[0054] The fitted peak wavelengths of the scattering spectra
recorded from the NCAs analyzed in FIG. 9 are plotted as function
of n in FIG. 10. The original spectra are included as inset. The
peak wavelength (.lamda..sub.res) strongly red-shifts from 558 nm
for n=1.3 to 670 nm for n=4.4. However, further increases of the
cluster size beyond n=4.4 lead to only small additional redshifts
of the scattering band. The peak wavelength increases only by 9 nm
to .lamda..sub.res=679 between n=4.4 and n=19.1.
[0055] The strong red-shift of the spectral response with
increasing degree of particle clustering is a direct consequence of
near-field coupling between the particles in the clusters, driven
by the increasing number of interstitial junction plasmons as n
increases. Plasmon hybridization between adjacent particle plasmons
leads to coupled cluster resonances that are energetically
stabilized with regard to the isolated particle plasmons. FIG. 10
shows that this stabilization saturates in larger clusters. The
major portion of the energy stabilization is reached for an average
cluster size of n=4.4. A further increase in the size of the
clusters results in only a small additional shift of their plasmon
resonance wavelength.
[0056] The red-shift due to plasmon hybridization stabilizing at
around n.gtoreq.4 may be understood from simple geometric
considerations. A close inspection of the SEM images of the
fabricated NCAs reveals that the clusters with n=4 preferentially
assume a rhombohedral geometry in which the inter-particle
distances are minimized (see FIG. 5 image (f)). Four gold
nanoparticles at the edges of a rhombus form the unit cell of a
monolayer of hexagonal closed packed spheres. This highly symmetric
arrangement enables the particles to minimize the total
inter-particle separation resulting in very efficient plasmon
coupling. The fact that an increase in the average cluster size
beyond n=4 does not lead to substantial additional spectral shifts
in regular arrays therefore implies that the coupling in the larger
clusters is dominated by inter-particle coupling between nearest
neighbors in the unit cell.
[0057] In three-dimensional clusters additional interactions
between particles along the third spatial axis (out-of-plane) can
shift the plasmon resonance further into the red than observed here
for two-dimensional clusters. In fact, some of the fabricated NCAs
contain contaminations with larger three dimensional agglomerates
due to imperfections during the fabrication process. Shoulders at
wavelengths>800 nm in the scattering spectra in FIG. 10 are
ascribed to these three-dimensional clusters and fractal particle
assemblies. The occasional contamination of the fabricated NCAs
does, however, not influence the observed global trends: the peak
wavelength red-shifts in the interval 1<n<4 and converges to
its maximum at n.apprxeq.4.
[0058] Assembly of Regular Nanoparticle Cluster Arrays.
[0059] FIG. 11 illustrates a template guided self-assembly process
to generate regular two-dimensional arrays of contiguous
nanoparticles. In the first step a photoresist (PMMA) film is
formed on a glass substrate covered by a thin noble metal layer
(e.g., 10 nm gold film). A regular structure of wells or openings
is then created in the PMMA film using e-beam lithography. The
resulting PMMA mask covers all parts of the surface except for the
anticipated binding sites. The binding sites are functionalized by
assembly of a monolayer of amino-terminated PEGs
(thiol-(CH2).sub.11EG.sub.7-amine, EG=ethylene glycol) on the
exposed gold surface. Under appropriate buffer conditions (pH<9)
the monolayer is positively charged. Thus, negatively charged 40 nm
colloidal gold nanoparticles readily bind on these positively
charged binding sites in an electrostatic guided self-assembly
process. The gold nanoparticles are passivated with a monolayer of
carboxy terminated PEGs (thiol-EG.sub.7-propionat, see FIG. 12).
The charged polymers on the gold surfaces serve two purposes; they
facilitate an efficient charge directed cluster assembly on the
template and function as insulating dielectric between the
particles and between the nanoparticles and the gold support.
[0060] SERS Performance of Nanoparticle Cluster Arrays.
[0061] Fabrication procedures that provide spatial control on the
nanoscale are instrumental in developing SERS substrates according
to rational design criteria. Our motivation for assembling arrays
of clusters of nearly touching gold nanoparticles at defined
locations is to reproducibly create hot-spots with high surface
density to generate SERS substrates with high enhancement factors
and improved enhancement reproducibility. The spectral
characterization of the fabricated cluster arrays has already
indicated the existence of both inter-particle and inter-cluster
plasmon coupling in NCAs. In order to be able to utilize the
interplay of electromagnetic interactions between individual
nanoparticles in the clusters and between clusters, it is important
to characterize the influence of the array specific geometry
parameters .LAMBDA. and n on the relative Raman intensities. All
SERS spectra in this study were excited at 785 nm. This wavelength
has been shown to minimize autofluorescence from biological
samples.
[0062] Influence of the Cluster Edge-to-Edge Separation .LAMBDA. on
the SERS Signal.
[0063] Following other SERS studies, paramercaptoaniline (pMA) was
used here as a test analyte to quantify the influence of .LAMBDA.
on the NCA Raman signal enhancement. To optimize the SERS
performance of the substrates, the SERS signal dependence on pump
power P (P=0.4 mW-7.3 mW) and data acquisition time t (t=10-60
seconds) was investigated first (see FIGS. 21 and 22). The pMA SERS
intensity on a .LAMBDA.=200 nm, D=200 nm NCA was found to be
linearly dependent on P and t. SERS substrates with good/excellent
signal to noise are obtained with 10 seconds of data collection and
P=1.8 mW. All SERS measurements subsequently reported were
performed with these acquisition parameters unless otherwise
stated.
[0064] FIG. 13 contains pMA SERS spectra from NCAs with different
values of .LAMBDA. but with fixed nanoparticle binding sizes (D=200
nm). Two effects contribute to the strong increase of the SERS
intensity with decreasing edge-to-edge separation. Firstly, the
density of the nanoparticle clusters and therefore the total SERS
active area in the scattering volume increases with decreasing A.
Secondly, the NCA scattering spectra as function of inter-cluster
separation (FIG. 8) reveals that for short edge-to-edge separations
.LAMBDA.<200 nm additional inter-cluster coupling further
enhances the local |E|-field. Such a local field enhancement effect
is evident from a comparison of SERS enhancement factors, G, as
function of . The absolute values of these enhancement factors are
only approximates since the surface coverage with pMA, the
accessible surface area of the clusters, the contribution of the
gold substrate, as well as the number of molecules in the reference
sample have to be estimated (see Methods section). Nevertheless,
they facilitate a quantitative comparison of the relative SERS
performances of samples with different filling fractions (i.e.
different ), prepared under otherwise identical conditions. The
approximate enhancement factors obtained for the 1077 cm.sup.1 band
of pMA on NCAs with constant binding site diameter (D=200 nm) are
plotted as function of .LAMBDA. in FIG. 14.
[0065] The measured SERS enhancement decreases from 2.210.sup.5 for
.LAMBDA.=50 nm to 110.sup.5 for .LAMBDA.=200 nm. For even larger
edge-to-edge separations the SERS enhancement is essentially
independent of A. The gain in G at short inter-cluster separations
is in agreement with the observed spectral red-shift of the plasmon
resonance and is consistent with increasing near-field interactions
between the clusters for .LAMBDA.<200 nm. The near-field
inter-cluster interactions increase the SERS enhancement generated
by individual clusters by a factor of .about.2 with respect to the
isolated clusters at the smallest .LAMBDA. tested in this study.
This observation further corroborates that plasmon coupling in NCAs
occurs on two relevant length scales: inter-particle in the
clusters and inter-cluster in the arrays.
[0066] Influence of Cluster Size on the SERS Signal.
[0067] The SERS enhancement as a function of the binding size
diameter D, and thus the average cluster size, n, in NCAs with
fixed edge-to-edge separation .LAMBDA. have also been
investigated.
[0068] In FIG. 15 the SERS intensities of the pMA 1077 cm.sup.-1
transition of NCAs with five different D values (50 nm, 80 nm, 100
nm, 130 nm, 200 nm) and fixed edge-to-edge separation (.LAMBDA.=200
nm) are compared. All of these nanoparticle cluster arrays were
fabricated on the same chip to minimize intensity effects due to
pMA concentration or pump power variability. The recorded signal
intensity increases nearly linearly with growing D. It is a priori
unclear how much a potential cluster size dependence of the SERS
enhancement contributes to the signal intensity gain. The influence
of D on the SERS enhancement was therefore estimated by calculating
the SERS enhancement factors G as function of .LAMBDA. (see FIG.
16). The general trend in FIG. 16 indicates that G does not
continuously increase with cluster size n but converges against a
maximum enhancement at n.apprxeq.3-4. In the case of NCAs with
fixed .LAMBDA.=200 nm a maximum enhancement factor of
G.apprxeq.110.sup.-5 is reached. This behavior corroborates the
trends observed for the plasmon resonance wavelength.
[0069] The stagnation of the resonance wavelength at n.apprxeq.4 in
FIG. 10 is rationalized by a maximization of the inter-particle
near-field interactions in compact cluster geometries. In addition,
in clusters of three or four particles with triangular or rhombal
cluster geometry all particles can be arranged in a "first
coordination shell" around a central cavity. Analyte molecules
located in this space can potentially experience very high local
fields leading to strong SERS enhancements highlighting the value
of the first interstitial coordination shell for SERS signal
enhancement.
[0070] Benchmarking NCAs with Competing SERS Substrates Using
Paramercaptoaniline (pMA) as Test Substance.
[0071] Performance of NCAs was evaluated by direct comparison with
two commonly used SERS substrates: (1) non-patterned 40 nm gold
nanoparticle films and (2) periodic two-dimensional arrays of gold
nanodisc arrays. The non-patterned colloid films were conveniently
generated on the same substrate next to the nanoparticle cluster
arrays by simply removing the photoresist from a large area in the
vicinity of the surface pattern during the e-beam writing step. All
subsequent processing steps were identical to the procedures
described above for the production of the nanocluster particle
arrays. The nanodisc arrays were fabricated on a 10 nm thin gold
film following standard procedures. In short, a PMMA mask was
generated on top of a gold coated glass slide using direct writing
e-beam lithography with subsequent development. Then a 40 nm thick
gold layer was thermally evaporated onto the patterned surface and
the PMMA mask was removed in a final lift-off step releasing the
regular gold nanodisc pattern. The NCAs used for the benchmarking
had an edge-to-edge separation of .LAMBDA.=200 nm and a cluster
binding size of D=200 nm. The nanofabricated nanodisc arrays had
the same edge-to-edge distance and total diameter as the NCAs.
[0072] The relative SERS performance of these three different
substrates was evaluated in direct comparison under identical
conditions. The Raman enhancement (G) factors at 785 nm for the
1077 cm.sup.1 mode of pMA and corresponding representative SEM
images of the investigated substrates are summarized in FIG. 17.
These values correspond to twelve measurements from three different
substrates for each substrate type. The NCA substrates yielded the
overall largest G values (.about.1.110.sup.5), followed by the
non-patterned nanoparticle film (.about.7.010.sup.4). The nanodisc
array exhibited the smallest G values (.about.1.010.sup.4). NCAs
with .LAMBDA.=50 nm, D=200 nm yielded the maximal enhancement
factor of G=2.210.sup.5 for the substrates prepared for this study
(see FIG. 14). These values fall within the typical SERS
enhancement range of gold island films and are comparable with
those obtained with other engineered SERS substrates such as gold
nanohole arrays. Although enhancement factors up to 1.10.sup.8 have
been observed in some cases for e-beam patterned gold nanodisc
arrays fabricated on a gold film, the NCAs are found to provide a
significantly higher (.about.1 order of magnitude) Raman signal
enhancement than the corresponding nanodisc arrays at least for pMA
excited at 785 nm.
[0073] The observation that the NCAs provide a stronger SERS signal
enhancement than smooth nanodisc arrays is readily ascribed to the
fact that the NCAs have a much higher degree of roughness than the
nanodiscs due to crevices, holes, and junctions between the
nanoparticles in the clusters. The incident field can be
effectively localized in these nano-roughened structures leading to
overall higher enhancement factors. Nanoscale roughness cannot,
however, account for the fact that the ensemble averaged SERS
enhancement factors for NCAs are also slightly higher than those of
the non-patterned gold nanoparticle films which also contain
nanoparticle clusters. Based on the observed dependencies of the
ensemble averaged G factors on .LAMBDA. and n, we propose the
following model to account for the observed differences between
patterned and non-patterned colloid substrates: the SERS
enhancement factors of individual two dimensional nanoparticle
clusters saturates at around n.apprxeq.4. In an array of patterned
clusters the total SERS enhancement of the individual clusters can
be further increased through cumulative electrodynamic interactions
occurring on two different length scales. On the length scale of a
few tens of nanometers (inter-cluster length scale) the cluster
plasmons couple and provide a first stage of strong enhancement of
the incident electric field. This enhanced field is then further
increased by the intra-cluster coupling between the individual
particles of the clusters. The phenomenon therefore consists of a
sequential enhancement similar to the effects observed in RF Yagi
antennas or optical nanolenses.
[0074] As discussed elsewhere, the Raman signal enhancement can be
maximized by matching the excitation wavelength with the absorption
band of the plasmonic SERS substrate. FIG. 18 shows the normalized
spectra of the investigated NCAs, smooth gold nanodisc arrays, and
non-patterned colloid films. Whereas NCAs and nanodisc arrays show
well defined plasmon resonances at around .about.700 nm with a full
width at half maximum (FWHM) extending from 630 nm to 780 nm, the
non-patterned colloid film shows a much broader scattering spectrum
with FWHM between 580 nm and 880 nm. We ascribe this spectral
broadening to a wide distribution of cluster sizes including
three-dimensional and fractal aggregates in the non-patterned
colloid film.
[0075] The measured relative Rayleigh scattering intensities for
NCAs and nanodisc arrays have decreased to .about.30% of their peak
intensity value at the SERS excitation wavelength of 785 nm. In
contrast, for the non-patterned colloid substrate the scattering
cross-section is close to its peak value at the 785 nm SERS
excitation wavelength. The detuning between 785 nm Raman excitation
wavelength and the resonance scattering maxima evident in FIG. 18
suggests that--unlike the non-patterned colloid films--the
enhancement factors of NCAs can be significantly increased by
improving the match between resonance wavelength and excitation
laser wavelength. This can be achieved either by using a laser with
an emission wavelength around 700 nm or by shifting the resonance
wavelength of the arrays closer to 785 nm by exchanging spherical
40 nm gold nanoparticles with building blocks that have
energetically lower particle resonances.
[0076] Another important SERS performance characteristic, in
addition to the signal enhancement, is the reproducibility of the
enhancement factors generated by different SERS substrates. The
reproducibility of the enhancement factor (G) is captured here by
the coefficient of variability, i.e. the standard deviation of G
values given by twelve independent measurements on four different
substrates, expressed as a percentage of the mean G value for each
substrate type. These variability coefficients are given in FIG.
17. As evident from this measure for G reproducibility, the
variations of the NCAs enhancement (12%) is much smaller than that
observed on either the non-patterned colloid substrate (41%), or
the nanodiscs (56%). Thus both in terms of absolute enhancement and
repeatability, the NCAs outperform the other two substrate types
tested here.
[0077] SERS Bacterial Detection and Identification Using NCAs.
[0078] The SERS performance of NCAs makes them potentially useful
candidates for complex sensing applications such as whole cell
fingerprinting. There is currently significant interest in
developing SERS for the rapid characterization and identification
of bacterial pathogens. Due to the distance dependence of the field
enhancement, SERS selectively probes the molecular components of
the outer layer of bacterial cells where chemical distinctions
appear to be the greatest thus enhancing specificity and may
therefore be a promising tool for bacterial diagnostics. The
successful application of SERS for this analytical application
requires substrates that can provide strong and reproducible
enhancements for these organisms at the single cell level and have
a storable shelf life in the 6-12 month range.
[0079] In the case of bacteria the surface morphology and the
binding affinity to the substrate are extremely important and can
influence both the detected vibrational bands and the total signal
intensities. Not all SERS active substrates provide SERS spectra of
whole bacterial cells. Only if a bacterial cell can effectively
attach to the surface such that characteristic surface moieties are
near SERS active sites will a strong Raman signal be observed.
[0080] In order to test the ability of the NCAs, the non-patterned
colloid films, and the nanodiscs to act as effective substrates for
the observation of SERS spectra of vegetative bacterial cells,
suspensions of three different bacterial species (Staphylococcus
aureus, Escherichia coli, and Bacillus cereus) were placed on these
substrates and SERS spectra excited at 785 nm (4.3 mW power, 10
seconds integration time) were acquired. Only very weak bacterial
SERS spectra could be observed on the nanodisc arrays. However,
both patterned (NCA) and non-patterned colloid substrates provided
quantifiable SERS signals. FIG. 19 shows representative SERS
spectra (single scan) of S. aureus, E. coli, and B. cereus obtained
from NCA and smooth (40 nm height) nanodisc arrays, both with 200
nm diameter and 200 nm edge-to-edge separation features. The
non-patterned gold nanoparticle substrates were located on the same
chip in close vicinity to the nanoparticle cluster array to ensure
that these two samples were always measured under identical
experimental conditions. As seen in FIG. 19, SERS spectra of
bacteria are stronger on the NCA substrates than on the
non-patterned colloid films. Furthermore, in contrast to the NCA
bacterial spectra, the bacterial SERS signal intensities exhibited
strong variations at different positions on the non-patterned
substrates.
[0081] The bacterial SERS spectra share many common spectral
features as evident in FIG. 19 and has been discussed previously.
Identification of the chemical species responsible for the
vibrational bands in the SERS spectra of bacteria has not yet been
achieved and is beyond the scope of this current report. However,
the unique SERS vibrational signatures provide the basis for a
rapid bacterial identification methodology when combined with
multivariate library searching techniques.
[0082] The ability of NCAs to be used for bacterial diagnostics is
demonstrated in FIG. 20. It has been shown previously how a
principal component analysis (PCA) based on the sign of the second
derivative of the SERS spectra provides improved specificity for
the identification of bacterial species and strains. The SERS
spectra are thus reduced to a series of zeroes and ones, i.e.
barcodes, as input for unsupervised clustering algorithms such as
PCA, or consequent supervised methods such as discriminant function
analysis, hierarchical cluster analysis, or neural network
techniques. Here the specificity of these SERS based signatures on
NCAs is shown by the results of a discriminant function analysis
(DFA) based on the barcode reduced SERS spectra of S. aureus, E.
coli, and B. cereus. The discriminant functions are linear
combinations of the first four PCs which capture 98% of the
variance of this 30 spectra data set.
[0083] As seen in the DF2 vs. DF1 plot in FIG. 20, the SERS
signature of B. cereus, E. coli, and S. aureus obtained on the NCAs
are well separated forming non-overlapped regions. The rings in
each of the cluster regions correspond to tow dimensional standard
deviations centered on the mean for each species cluster. The large
standard deviation separations between these clusters of the tested
clusters of the tested bacteria indicate that the NCAs enable a
spectral signature capable of bacterial identification. Work is
currently ongoing to determine the best performing SERS substrates
for this purpose based on the criteria of Raman enhancement
strength, spectral reproducibility, substrate storage lifetime and
commercial scalability.
[0084] It is shown that nanoparticle cluster arrays (NCAs) provide
reproducible SERS signals from different bacteria species including
Bacillus cereus, Escherichia coli, and Staphylococcus aureus. The
NCAs enabled a spectroscopic discrimination of these samples
through SERS in combination with multivariate data analysis
techniques. The NCAs used for this analytical challenge were
fabricated by combining top down nanofabrication and bottom-up self
assembly procedures in a template guided self-assembly process.
This approach provides control over the size of the particle
clusters and their spatial location on the nanoscale. We used this
process to fabricate regular arrays of 40 nm gold nanoparticle
clusters of defined cluster size n and cluster edge-to-edge
separation .LAMBDA. over several hundred square microns. The
photonic-plasmonic scattering resonances of the arrays as function
of n and .LAMBDA. were characterized. The spectra are dominated by
the ensemble resonance of the gold film supported nanoparticle
clusters at large cluster separations. For NCAs with short
inter-cluster separations, .LAMBDA.<200 nm, we also observe an
additional short wavelength band which we ascribe to light
diffraction from the NCAs acting as transmission grating for the
incident light. A systematic variation of .LAMBDA. revealed that
the plasmon resonance peak red-shifts with decreasing .LAMBDA. for
edge-to-edge separations .LAMBDA..ltoreq.200 nm indicating
additional inter-cluster near-field interactions. The red-shift of
the plasmon resonance is accompanied by an increase in the SERS
enhancement for .LAMBDA..ltoreq.200 nm. This observation confirms
that electrodynamic interactions between the clusters can further
increase the Raman signal intensity generated by individual
isolated clusters, and we conclude that the net enhancement is the
result of a multiscale field enhancement in NCAs. Next to the
edge-to-edge separation, the SERS signal enhancement also depends
on the cluster size n, and we investigated the optical response and
the SERS enhancement of NCAs as function of n. The cluster
resonances of the arrays strongly red-shift with increasing cluster
size n up to n.apprxeq.4. We did not observe a further significant
increase in the enhancement for larger two-dimensional nanoparticle
clusters. Similarly, the Raman signal enhancement shows a
significant increase with growing cluster size for small cluster
sizes n.gtoreq.4 but remains essentially constant for larger
cluster sizes. This behavior suggests that for n=4 cluster
structures are accessible which enable very efficient plasmon
coupling between all particles of the clusters. We find that one of
the preferred structures for n=4 is the rhombus which is the unit
cell of a two-dimensional close packing. The size dependency of the
SERS enhancement indicates a dominance of the interstitial first
shell in the Raman signal amplification.
[0085] Overall, it is revealed that NCAs can be used to engineer
SERS substrates whose spectral and field localization properties
can be controlled systematically by varying n and .LAMBDA.. We
benchmarked NCAs with non-patterned two-dimensional gold
nanoparticle substrates and regular gold nanodisc arrays. We found
that NCAs offer a good compromise between signal enhancement and
substrate reproducibility. In addition, the NCAs clearly
outperformed the other substrates in SERS measurements of bacteria.
Future steps for further improvement and optimization of the SERS
enhancement by NCAs will involve the study of the nanoparticle
composition, size, and shape as well as the geometric patterns of
the arrays.
[0086] While various embodiments of the invention have been
particularly shown and described, it will be understood by those
skilled in the art that various changes in form and details may be
made therein without departing from the spirit and scope of the
invention as defined by the appended claims.
* * * * *