U.S. patent application number 15/701311 was filed with the patent office on 2018-03-29 for pharmaceutical compositions comprising an antipsychotic drug and a vmat2 inhibitor and uses thereof.
The applicant listed for this patent is Neurocrine Biosciences, Inc. Invention is credited to Samuel Roger Jesse Hoare.
Application Number | 20180085364 15/701311 |
Document ID | / |
Family ID | 52589782 |
Filed Date | 2018-03-29 |
United States Patent
Application |
20180085364 |
Kind Code |
A1 |
Hoare; Samuel Roger Jesse |
March 29, 2018 |
PHARMACEUTICAL COMPOSITIONS COMPRISING AN ANTIPSYCHOTIC DRUG AND A
VMAT2 INHIBITOR AND USES THEREOF
Abstract
New methods of treating schizophrenia and schizoaffective
disorder by administration of pharmaceutical compositions
comprising an antipsychotic compound and a VMAT2 inhibitor to a
subject in need thereof are provided.
Inventors: |
Hoare; Samuel Roger Jesse;
(San Diego, CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Neurocrine Biosciences, Inc |
San Diego |
CA |
US |
|
|
Family ID: |
52589782 |
Appl. No.: |
15/701311 |
Filed: |
September 11, 2017 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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15116786 |
Aug 4, 2016 |
9782398 |
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PCT/US2015/014893 |
Feb 6, 2015 |
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15701311 |
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61937223 |
Feb 7, 2014 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A61P 25/18 20180101;
A61K 31/519 20130101; A61K 31/551 20130101; A61K 31/473 20130101;
A61K 31/5513 20130101; A61P 25/24 20180101; A61K 31/4745 20130101;
A61P 43/00 20180101; A61K 45/06 20130101; A61K 31/519 20130101;
A61K 2300/00 20130101; A61K 31/551 20130101; A61K 2300/00 20130101;
A61K 31/4745 20130101; A61K 2300/00 20130101 |
International
Class: |
A61K 31/473 20060101
A61K031/473; A61K 31/5513 20060101 A61K031/5513; A61K 45/06
20060101 A61K045/06; A61K 31/519 20060101 A61K031/519; A61K 31/551
20060101 A61K031/551; A61K 31/4745 20060101 A61K031/4745 |
Claims
1. A method for treating a neuropsychiatric disorder in a subject
comprising administering to the subject (a) an antipsychotic drug
and (b) a VMAT2 inhibitor, wherein the therapeutically effective
amount of the antipsychotic drug administered to the subject is
less than the therapeutically effective amount of the antipsychotic
drug when administered in the absence of the VMAT2 inhibitor.
2-17. (canceled)
18. A pharmaceutical preparation for use in treating a
neuropsychiatric disorder, said preparation comprising an
antipsychotic drug and a VMAT2 inhibitor, wherein the preparation
comprises an amount of the antipsychotic drug that is a
subtherapeutic amount if used in the absence of the VMAT2
inhibitor.
19-32. (canceled)
33. A method for enhancing efficacy of an antipsychotic drug
comprising administering to a subject a combination of (a) the
antipsychotic drug, and (b) a VMAT2 inhibitor.
34-46. (canceled)
47. A pharmaceutical preparation comprising an antipsychotic drug
and a VMAT2 inhibitor, wherein the preparation is effective for
treating a neuropsychiatric disorder, and wherein the amount of the
antipsychotic drug is subtherapeutic compared with the therapeutic
amount of the antipsychotic drug when used alone for treating the
neuropsychiatric disorder in the absence of the VMAT2
inhibitor.
48. A pharmaceutical preparation comprising synergistically
effective amounts of an antipsychotic drug and a VMAT2
inhibitor.
49-60. (canceled)
61. A vesicular monoamine transport 2 (VMAT2) inhibitor that is
[(2R,3S,11bR)-9,10-dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol, wherein the VMAT2 inhibitor
is for the treatment of tardive dyskinesia in a subject in need
thereof who is receiving an antipsychotic drug for the treatment of
a neuropsychiatric disorder.
62. A vesicular monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the VMAT2 inhibitor is for the
treatment of tardive dyskinesia in a subject in need thereof who is
receiving an antipsychotic drug for the treatment of a
neuropsychiatric disorder.
63. A vesicular monoamine transport 2 (VMAT2) inhibitor that is a
toluenesulfonate salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the VMAT2 inhibitor is for the
treatment of tardive dyskinesia in a subject in need thereof who is
receiving an antipsychotic drug for the treatment of a
neuropsychiatric disorder.
64. A first pharmaceutical preparation for the treatment of tardive
dyskinesia, said first pharmaceutical preparation comprising a
vesicular monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the first pharmaceutical
preparation is to be used in combination with a second
pharmaceutical preparation for the treatment of a neuropsychiatric
disorder, and wherein the second pharmaceutical preparation
comprises an antipsychotic drug.
65. A first pharmaceutical preparation for the treatment of tardive
dyskinesia, said first pharmaceutical preparation comprising a
vesicular monoamine transport 2 (VMAT2) inhibitor that is a
toluenesulfonate salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the first pharmaceutical
preparation is to be used in combination with a second
pharmaceutical preparation for the treatment of a neuropsychiatric
disorder, and wherein the second pharmaceutical preparation
comprises an antipsychotic drug.
66. A method for treating a neuropsychiatric disorder in a subject
in need thereof, comprising: administering to the subject an
antipsychotic drug for the treatment of a neuropsychiatric disorder
and a vesicular monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
67. A method for treating a neuropsychiatric disorder in a subject
in need thereof, comprising: administering to the subject an
antipsychotic drug for the treatment of a neuropsychiatric disorder
and a vesicular monoamine transport 2 (VMAT2) inhibitor that is a
toluenesulfonate salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
68. A method of treating tardive dyskinesia in a subject in need
thereof, comprising: administering to the subject a vesicular
monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the subject is also being
administered an antipsychotic drug for the treatment of a
neuropsychiatric disorder.
69. A method of treating tardive dyskinesia in a subject in need
thereof, comprising: administering to the subject a vesicular
monoamine transport 2 (VMAT2) inhibitor that is a toluenesulfonate
salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the subject is also being
administered an antipsychotic drug for the treatment of a
neuropsychiatric disorder.
70. A method of treating tardive dyskinesia in a subject in need
thereof, comprising: administering to the subject a vesicular
monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the subject has schizophrenia,
schizoaffective disorder, bipolar disorder, or major depressive
disorder.
71. A method of treating tardive dyskinesia in a subject in need
thereof, comprising: administering to the subject a vesicular
monoamine transport 2 (VMAT2) inhibitor that is a toluenesulfonate
salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester, wherein the subject has schizophrenia,
schizoaffective disorder, bipolar disorder, or major depressive
disorder.
72. A method of reducing the frequency of occurrence of tardive
dyskinesia associated with administration of an antipsychotic drug
in a subject in need thereof, comprising: administering to the
subject a vesicular monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
73. A method of reducing the frequency of occurrence of tardive
dyskinesia associated with administration of an antipsychotic drug
in a subject in need thereof, comprising: administering to the
subject a vesicular monoamine transport 2 (VMAT2) inhibitor that is
a toluenesulfonate salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
74. A method of reducing the severity or intensity of a movement
disorder associated with administration of an antipsychotic drug in
a subject in need thereof, comprising: administering to the subject
a vesicular monoamine transport 2 (VMAT2) inhibitor that is
(S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
75. A method of reducing the severity or intensity of a movement
disorder associated with administration of an antipsychotic drug in
a subject in need thereof, comprising: administering to the subject
a vesicular monoamine transport 2 (VMAT2) inhibitor that is a
toluenesulfonate salt of (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
76. A method of treating tardive dyskinesia by inhibiting vesicular
monoamine transport 2 (VMAT2) in a subject in need thereof,
comprising: contacting the VMAT2 with a therapeutically effective
amount of a VMAT2 inhibitor that is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), wherein the subject is being
administered an atypical antipsychotic drug for the treatment of a
neuropsychiatric disorder.
77. The method of claim 76, wherein the R,R,R DHTBZ results from
administration to the subject of a precursor of R,R,R, DHTBZ
wherein the precursor is (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
78. A method of treating tardive dyskinesia by inhibiting vesicular
monoamine transport 2 (VMAT2) in a subject in need thereof,
comprising: contacting the VMAT2 with a therapeutically effective
amount of a VMAT2 inhibitor that is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), wherein the subject has
schizophrenia, schizoaffective disorder, bipolar disorder, or major
depressive disorder.
79. The method of claim 78, wherein the R,R,R DHTBZ results from
administration to the subject of a precursor of R,R,R, DHTBZ
wherein the precursor is (S)-2-amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2yl ester.
80. A method of treating tardive dyskinesia in a subject in need
thereof, comprising: administering to the subject a vesicular
monoamine transport 2 (VMAT2) inhibitor that is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), wherein the subject is also
being administered an atypical antipsychotic drug for the treatment
of a neuropsychiatric disorder.
81. A method of treating tardive dyskinesia in a subject in need
thereof, comprising: administering to the subject a vesicular
monoamine transport 2 (VMAT2) inhibitor that is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), wherein the subject has
schizophrenia, schizoaffective disorder, bipolar disorder, or major
depressive disorder.
Description
BACKGROUND
Technical Field
[0001] Provided herein are methods of treating schizophrenia,
schizoaffective disorder, bipolar disease, major depressive
disorder and other conditions commonly treated with antipsychotic
medication by administering to a subject in need thereof a
pharmaceutical composition comprising an antipsychotic compound and
a VMAT2 inhibitor.
Description of the Related Art
[0002] Schizophrenia affects approximately 1% of the adult
population and reduces life expectancy by an average of 20 to 25
years through the impact of the disorder on self-care and physical
health, as well as through suicide. At the present time the
etiological mechanisms underlying schizophrenia are poorly
understood. Schizophrenia is diagnosed clinically, based on
characteristic symptoms of psychosis, disorganization and so called
`negative` symptoms (representing a reduced range of emotional
expression, reduced production of speech and a lack of
volition/motivation); duration of illness; impaired functioning;
and the exclusion of other disorders such as autism and bipolar
disorder. For clinicians, identifying which psychotic patients have
schizophrenia requires clinical acumen and familiarity with the
DSM-IV or ICD-10 diagnostic manuals (see, e.g., Corvin, BMC Biol.
2011; 9: 77).
[0003] Antipsychotic drug therapy is a pillar in the treatment of
schizophrenia. These antipsychotic drugs, also known as
neuroleptics, generally cause a reduction of the `positive`
symptoms of schizophrenia, namely psychosis, thought disorders, and
disorganized behavior. Antipsychotics generally have a lesser
influence on cognition and on the `negative` symptoms of the
disease, which include lack of motivation and emotion, social
withdrawal, lack of interest in everyday activities, and the
reduced ability to plan or carry out activities.
[0004] First generation or "typical" antipsychotics have been used
for over 50 years in the treatment of schizophrenia and other
psychotic disorders. The first marketed antipsychotic was
chlorpromazine; other typical antipsychotics include fluphenazine,
haloperidol, loxapine, molindone, perphenazine, pimozide,
sulpiride, thioridazine, and trifluoperazine. These typical
antipsychotics all gain their primary efficacy through D2 dopamine
receptor antagonism and have a propensity to cause movement
disorders including parkinsonism (tremor, rigidity, bradykinesia
and gait instability) as well as dystonia, dyskinesia (e.g.,
tardive dyskinesia), and akathisia.
[0005] Second generation or "atypical" antipsychotics were
developed, and these drugs possess a lower risk of causing TD and
related movement disorders with chronic administration. These drugs
include aripiprazole, asenapine, clozapine, iloperidone,
olanzapine, paliperidone, quetiapine, risperidone, and ziprasidone.
These atypical antipsychotics all exert their primary efficacy
through D2 dopamine receptor antagonism with additional effects on
receptors for other neurotransmitters. These atypical
antipsychotics are associated with metabolic side effects
sufficient to affect life expectancy. These side effects include a
propensity to induce weight gain, as well as related metabolic
disturbances such as hypertriglyceridemia and hyperglycemia.
Clozapine appears to be the most effective as treatment for severe
mental illness, but it has additional serious medical side effects,
including a significant incidence of agranulocytosis that requires
frequent monitoring of patients' white blood counts as a
requirement for using the drug.
[0006] In addition to treatment of schizophrenia and
schizoaffective disorder, certain antipsychotic medications have
been approved as treatments of bipolar disorder, major depressive
disorder (MDD), and autism spectrum disorders. Off-label use is
prevalent, particularly of atypicals, which are used for the
treatment of various conditions including anxiety,
attention-deficit hyperactivity disorder (ADHD), dementia,
depression, insomnia, obsessive-compulsive disorder (OCD),
post-traumatic stress disorder, substance abuse, and Tourette's
syndrome.
[0007] Because the side effects associated with administration of
antipsychotic medications can significantly impact a patient's
health and well-being, alternatives to the current therapies are
needed.
BRIEF SUMMARY
[0008] Briefly, this disclosure relates to the discovery that the
combination of an antipsychotic and a VMAT2 inhibitor shows
therapeutic synergy and improves the therapeutic index of the
antipsychotic in the treatment of neuropsychiatric disorders, such
as schizophrenia, schizoaffective disorder, bipolar disease, major
depressive disorder, and other conditions commonly treated with
antipsychotic medications. Provided herein are new methods of
treating diseases in patients who currently receive antipsychotics,
and to pharmaceutical compositions useful in the treatment of
neuropsychiatric disorders such as schizophrenia. More
specifically, the methods described herein involve the
administration of an antipsychotic and a VMAT2 inhibitor in
combination. The present disclosure provides the following
embodiments.
[0009] In one embodiment, a method is provided for treating a
neuropsychiatric disorder in a subject comprising administering to
the subject (a) an antipsychotic drug and (b) a VMAT2 inhibitor,
wherein the therapeutically effective amount of the antipsychotic
drug administered to the subject is less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor. In certain embodiments, the
neuropsychiatric disorder is schizophrenia, schizoaffective
disorder, bipolar disorder, major depressive disorder (MDD), or
autism. In particular embodiments, the antipsychotic drug and the
VMAT2 inhibitor are administered concurrently. In other certain
embodiments, the antipsychotic drug and the VMAT2 inhibitor are
formulated in the same pharmaceutical composition. In another
specific embodiment, the antipsychotic drug is formulated in a
first pharmaceutical composition and the VMAT2 inhibitor is
formulated in a second pharmaceutical composition. In certain
embodiments, the antipsychotic drug is a typical antipsychotic
drug. In a more specific embodiment, the typical antipsychotic drug
is fluphenazine, haloperidol, loxapine, molindone, perphenazine,
pimozide, sulpiride, thioridazine, or trifluoperazine. In yet
another embodiment, the antipsychotic drug is an atypical
antipsychotic drug. In a specific embodiment, the atypical
antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone. In a particular embodiment, the therapeutically
effective amount of the antipsychotic drug is 10 to 90% less than
the therapeutically effective amount of the antipsychotic drug when
administered in the absence of the VMAT2 inhibitor. In an
embodiment, the therapeutically effective amount of the
antipsychotic drug is at least 25% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor. In an embodiment, the
therapeutically effective amount of the antipsychotic drug is at
least 50% less than the therapeutically effective amount of the
antipsychotic drug when administered in the absence of the VMAT2
inhibitor. In a particular embodiment, the VMAT2 inhibitor is
tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one). In another specific embodiment, the VMAT2 inhibitor
is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), and precursors thereof. In
yet another specific embodiment, the VMAT2 inhibitor is
(S)-2-Amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester. In another embodiment, the VMAT2
inhibitor is deuterated tetrabenazine, particularly
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ). In another embodiment, the VMAT2
inhibitor is
[(2R,3S,11bR)-9,10-dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol or a precursor thereof.
[0010] In one embodiment, a pharmaceutical preparation is provided
for use in treating a neuropsychiatric disorder, the preparation
comprising an antipsychotic drug and a VMAT2 inhibitor, wherein the
preparation comprises an amount of the antipsychotic drug that is a
subtherapeutic amount if used in the absence of the VMAT2
inhibitor. In a particular embodiment, the neuropsychiatric
disorder is schizophrenia, schizoaffective disorder, bipolar
disorder, major depressive disorder (MDD), or autism. In a certain
embodiment, the antipsychotic drug is a typical antipsychotic drug.
In a certain particular embodiment, the typical antipsychotic drug
is fluphenazine, haloperidol, loxapine, molindone, perphenazine,
pimozide, sulpiride, thioridazine, or trifluoperazine. In another
particular embodiment, the antipsychotic drug is an atypical
antipsychotic drug. In a more specific embodiment, the atypical
antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone. In another embodiment, the antipsychotic drug is
formulated in a first pharmaceutical composition and the VMAT2
inhibitor is formulated in a second pharmaceutical composition. In
a particular embodiment, the therapeutically effective amount of
the antipsychotic drug is 10 to 90% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor. In still another specific
embodiment, the therapeutically effective amount of the
antipsychotic drug is at least 25% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor. In still another specific
embodiment, the therapeutically effective amount of the
antipsychotic drug is at least 50% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor. In one particular embodiment, the
VMAT2 inhibitor is tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one). In yet another particular embodiment, the VMAT2
inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof. In
still another certain embodiment, the VMAT2 inhibitor is
(S)-2-Amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester. In another embodiment, the VMAT2
inhibitor is deuterated tetrabenazine, particularly
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ). In another embodiment, the VMAT2
inhibitor is
[(2R,3S,11bR)-9,10-dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol or a precursor thereof.
[0011] Also provided herein in another embodiment, is a method for
enhancing efficacy of an antipsychotic drug comprising
administering to a subject a combination of (a) the antipsychotic
drug, and (b) a VMAT2 inhibitor. In one embodiment, enhancing
efficacy of the antipsychotic drug comprises decreasing the amount
of the antipsychotic drug that is therapeutically effective. In a
particular embodiment, the therapeutically effective amount of the
antipsychotic drug is 10 to 90% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor. In a specific embodiment, the
amount of the antipsychotic drug that is therapeutically effective
is at least 25% less than the amount of the antipsychotic drug that
is therapeutically effective when the antipsychotic drug is
administered in the absence of the VMAT2 inhibitor. In another
specific embodiment, the amount of the antipsychotic drug that is
therapeutically effective is at least 50% less than the amount of
the antipsychotic drug that is therapeutically effective when the
antipsychotic drug is administered in the absence of the VMAT2
inhibitor. In one embodiment, the antipsychotic drug is a typical
antipsychotic drug. In a specific embodiment, the typical
antipsychotic drug is fluphenazine, haloperidol, loxapine,
molindone, perphenazine, pimozide, sulpiride, thioridazine, or
trifluoperazine. In another embodiment, the antipsychotic drug is
an atypical antipsychotic drug. In a more specific embodiment, the
atypical antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone. In another specific embodiment, the VMAT2 inhibitor is
tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one). In still another specific embodiment, the VMAT2
inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof. In
another particular embodiment, the VMAT2 inhibitor is
(S)-2-Amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester. In another embodiment, the VMAT2
inhibitor is deuterated tetrabenazine, particularly
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ). In another embodiment, the VMAT2
inhibitor is
[(2R,3S,11bR)-9,10-dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol or a precursor thereof.
[0012] In another embodiment, a pharmaceutical preparation is
provided that comprises an antipsychotic drug and a VMAT2
inhibitor, wherein the preparation is effective for treating a
neuropsychiatric disorder, and wherein the amount of the
antipsychotic drug is subtherapeutic compared with the therapeutic
amount of the antipsychotic drug when used alone for treating the
neuropsychiatric disorder in the absence of the VMAT2 inhibitor. In
another embodiment, a pharmaceutical preparation is provided that
comprises synergistically effective amounts of an antipsychotic
drug and a VMAT2 inhibitor. In particular embodiments of the
preparations, the antipsychotic drug is a typical antipsychotic
drug. In specific embodiments, the typical antipsychotic drug is
fluphenazine, haloperidol, loxapine, molindone, perphenazine,
pimozide, sulpiride, thioridazine, or trifluoperazine. In other
particular embodiments, the antipsychotic drug is an atypical
antipsychotic drug. In more specific embodiments, the atypical
antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone. In still more specific embodiments, the VMAT2
inhibitor is tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one). In other particular embodiments, the VMAT2 inhibitor
is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof. In
still other particular embodiments, the VMAT2 inhibitor is
(S)-2-Amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester. In another embodiment, the VMAT2
inhibitor is deuterated tetrabenazine, particularly
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ). In another embodiment, the VMAT2
inhibitor is
[(2R,3S,11bR)-9,10-dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol or a precursor thereof. In
other certain embodiments of the preparations, the antipsychotic
drug and the VMAT2 inhibitor are formulated in the same
pharmaceutical composition with at least one pharmaceutically
acceptable excipient. In still other particular embodiments, the
antipsychotic drug is formulated in a first pharmaceutical
composition with at least one pharmaceutically acceptable excipient
and the VMAT2 inhibitor is formulated in a second pharmaceutical
composition with at least one pharmaceutically acceptable
excipient. In certain embodiments, the neuropsychiatric disorder is
schizophrenia, schizoaffective disorder, bipolar disorder, major
depressive disorder (MDD), or autism.
[0013] These and other aspects of the invention will be apparent
upon reference to the following detailed description. To this end,
various references are set forth herein which describe in more
detail certain background information, procedures, compounds and/or
compositions, and are each hereby incorporated by reference in
their entirety.
[0014] Terms not specifically defined herein should be given the
meanings that would be given to them by one of skill in the art in
light of the disclosure and the context. As used in the
specification, however, unless specified to the contrary, the terms
have the meaning indicated.
[0015] Reference throughout this specification to "one embodiment"
or "an embodiment" means that a particular feature, structure or
characteristic described in connection with the embodiment is
included in at least one embodiment. Thus, the appearances of the
phrases "in one embodiment" or "in an embodiment" in various places
throughout this specification are not necessarily all referring to
the same embodiment. Furthermore, the particular features,
structures, or characteristics may be combined in any suitable
manner in one or more embodiments.
[0016] Also, as used in this specification and the appended claims,
the singular forms "a," "an," and "the" include plural referents
unless the content clearly dictates otherwise. Thus, for example,
reference to "a non-human animal" may refer to one or more
non-human animals, or a plurality of such animals, and reference to
"a cell" or "the cell" includes reference to one or more cells and
equivalents thereof (e.g., plurality of cells) known to those
skilled in the art, and so forth. When steps of a method are
described or claimed, and the steps are described as occurring in a
particular order, the description of a first step occurring (or
being performed) "prior to" (i.e., before) a second step has the
same meaning if rewritten to state that the second step occurs (or
is performed) "subsequent" to the first step. The term "about" when
referring to a number or a numerical range means that the number or
numerical range referred to is an approximation within experimental
variability (or within statistical experimental error), and thus
the number or numerical range may vary between 1% and 15% of the
stated number or numerical range. It should also be noted that the
term "or" is generally employed in its sense including "and/or"
unless the content clearly dictates otherwise. The term, "at least
one," for example, when referring to at least one compound or to at
least one composition, has the same meaning and understanding as
the term, "one or more."
BRIEF DESCRIPTION OF THE DRAWINGS
[0017] FIG. 1 shows the number of escapes in the Conditioned
Avoidance Response (CAR) model as an indicator for antipsychotic
activity. Vehicle, risperidone (0.1 mg/kg) singly, R,R,R DHTBZ
singly (0.15 mg/kg), and a combination of risperidone 0.1 mg/kg)
and R,R,R DHTBZ (0.15 mg/kg) were administered and the number of
escapes measured.
[0018] FIG. 2 shows the decrease in ED.sub.50 (escape response) of
the antipsychotic risperidone when administered in combination with
the VMAT2 inhibitor R,R,R DHTBZ (0.15 mg/kg) in a rat model.
[0019] FIG. 3 shows the avoidance response in the Conditioned
Avoidance Response (CAR) model as an indicator for antipsychotic
activity. Vehicle, olanzapine singly (0.6 mg/kg), R,R,R DHTBZ
singly, and a combination of olanzapine (0.6 mg/kg) and R,R,R DHTBZ
(0.15 mg/kg) were administered and the number of escapes
measured.
[0020] FIG. 4 illustrates the decrease in ED.sub.50 (escape
response) of the antipsychotic olanzapine when administered in
combination with the VMAT2 inhibitor R,R,R DHTBZ (0.15 mg/kg) in a
rat model.
[0021] FIG. 5 shows the number of escapes in the Conditioned
Avoidance Response (CAR) model as an indicator for antipsychotic
activity. Vehicle, risperidone (0.1 mg/kg) singly, Compound 5-1
singly (0.3 mg/kg), and a combination of risperidone 0.1 mg/kg) and
Compound 5-1 (0.3 mg/kg) (Cmpd 5-1) were administered and the
number of escapes measured.
[0022] FIG. 6 shows the decrease in ED.sub.50 (escape response) of
the antipsychotic risperidone when administered in combination with
the VMAT2 Compound 5-1 (0.3 mg/kg) in a rat model.
[0023] FIG. 7 shows the avoidance response in the Conditioned
Avoidance Response (CAR) model as an indicator for antipsychotic
activity. Vehicle, olanzapine singly (0.6 mg/kg), Compound 5-1 (0.3
mg/kg) (Cmpd 5-1) singly, and a combination of olanzapine (0.6
mg/kg) and Compound 5-1 (0.3 mg/kg) were administered and the
number of escapes measured.
[0024] FIG. 8 illustrates the decrease in ED.sub.50 (escape
response) of the antipsychotic olanzapine when administered in
combination with the VMAT2 inhibitor Compound 5-1 (0.3 mg/kg) in a
rat model.
DETAILED DESCRIPTION
[0025] A major drawback in treating schizophrenia and other
conditions commonly treated with an antipsychotic (also called a
neuroleptic) is the incidence of tardive dyskinesia (TD) that
results from the administration of the antipsychotic therapy.
Typical antipsychotic drugs (also called first generation
antipsychotics) have a high incidence of extrapyramidal side
effects, including tardive dyskinesia (TD). Second generation
antipsychotic drugs (also called atypical antipsychotics) possess a
lessened likelihood of causing TD but have other adverse effects
such as weight gain and metabolic disturbances. Recent studies
comparing the effectiveness of typical versus atypical
antipsychotics show little difference in effectively treating
psychosis even though the use of the second generation atypical
antipsychotics greatly exceeds the current use of typical
antipsychotics.
[0026] As disclosed herein, unexpectedly when a VMAT2 inhibitor is
administered with an antipsychotic drug, the amount of
antipsychotic administered may be reduced from the amount of the
antipsychotic that is commonly administered to a subject due to a
synergistic combination of the antipsychotic and the VMAT2
inhibitor. Administration of this combination results in more
potent antipsychotic activity than observed when the antipsychotic
drug is administered alone at the same dose. This reduction in dose
can reduce the risk of occurrence of tardive dyskinesia associated
with the antipsychotic drug, especially in the case of typical
antipsychotic administration. Reducing the administered dose of an
atypical antipsychotic agent by combining the atypical agent with a
VMAT2 inhibitor may lessen or reduce the likelihood of occurrence,
lessen or reduce time to onset of, and/or lessen or reduce the
severity of the metabolic side effects (e.g., weight gain) often
seen with these agents. In patients who have developed TD, the
reduction in antipsychotic drug dose along with the presence of a
potent VMAT2 inhibitor may thus be useful for treating TD while
maintaining antipsychotic efficacy that is commensurate with
antipsychotic efficacy observed when a higher dose of the
antipsychotic alone is administered.
[0027] The methods, uses, and compositions described herein may
have utility over a wide range of therapeutic applications, and may
be used to treat a variety of neuropsychiatric conditions in a
subject (i.e., patient, individual). For example, such conditions
include neuropsychiatric conditions (e.g., schizophrenia,
schizoaffective disorder, bipolar disease, major depressive
disorder (MDD), manic depressive disorder, depression with
psychotic features, delusional disorder and other psychotic
conditions, the symptoms of hallucinations and delusions), and
tardive dyskinesia. In a particular embodiment, methods are
provided herein for treating a neuropsychiatric condition (for
example, schizophrenia or schizoaffective disorder). Also provided
are pharmaceutical compositions useful in the treatment of the
aforementioned neuropsychiatric conditions, including schizophrenia
or schizoaffective disorder. In other certain embodiments, the
pharmaceutical compositions are useful in the treatment of a
neuropsychiatric condition and tardive dyskinesia.
Antipsychotic Drugs and VMAT2 Inhibitors
[0028] The first marketed antipsychotic was chlorpromazine in 1952.
This typical antipsychotic was followed by use of other typicals
including fluphenazine, haloperidol, loxapine, molindone,
perphenazine, pimozide, sulpiride, thioridazine, and
trifluoperazine, or example. These typical antipsychotics all gain
their primary efficacy through D2 dopamine receptor antagonism and
have a propensity to cause movement disorders including
parkinsonism (tremor, rigidity, bradykinesia and gait instability)
as well as dystonia, dyskinesia, and akathisia.
[0029] In large cohort studies, tardive dyskinesia (TD) has been
shown to affect at least one in five, and perhaps as many as one in
three, patients treated chronically with first-generation
antipsychotics. New onset (incidence) of TD is approximately 3% to
5% per year of treatment, and these rates are increased as much as
five-fold in elderly patients (see, e.g., Lencz et al., Dialogues
Clin Neurosci. 2009 December; 11(4): 405).
[0030] Tardive dyskinesia is a chronic disorder of the nervous
system, characterized by involuntary movements most often involving
the mouth, tongue, facial muscles, and to a lesser extent, the
trunk and extremities. Most cases of TD are caused by long-term use
of antipsychotic drugs. The condition can persist for months,
years, or even permanently. In addition to physical discomfort and
social stigma, presence of TD has been associated with reduced
quality of life, increased psychopathology, and increased mortality
rates. The etiology of TD is unknown, but antipsychotic drugs are
hypothesized to cause TD through their dopamine antagonism.
[0031] Second generation or atypical antipsychotics were developed,
and these drugs are believed to possess a lower risk of causing TD
and related movement disorders when chronically administered. The
incidence of TD with these drugs is as much as 80% lower than with
typical neuroleptics. However, atypical antipsychotics cause side
effects that include weight gain and related metabolic disturbances
such as hypertriglyceridemia and hyperglycemia. Examples of
atypical antipsychotic drugs include aripiprazole, asenapine,
clozapine, iloperidone, olanzapine, paliperidone, quetiapine,
risperidone, and ziprasidone. These atypical antipsychotics all
exert their primary efficacy through D2 dopamine receptor
antagonism with additional effects on receptors for other
neurotransmitters.
[0032] Table 1 lists examples of typical antipsychotics and the
range of recommended starting, target, and maximum daily doses for
a reference case of a moderately symptomatic adult man with
schizophrenia. Table 2 lists examples of atypical antipsychotics
and the range of recommended starting, target, and maximum daily
doses for a reference case of a moderately symptomatic adult man
with schizophrenia (see, e.g., Gardner et al., Am. J. Psychiatry
2010: 167:686-693).
TABLE-US-00001 TABLE 1 Recommended Oral Dosing of Typical
Antipsychotics (mg) Typical Starting Target Maximum fluphenazine 3
5-15 20 haloperidol 3 5-10 20 loxapine 17.5 20-100 200 molindone
22.5 50-188 225 perphenazine 8 12-24 42 pimozide 2 4-6 10 sulpiride
100 300-600 1000 thioridazine 88 200-500 800 trifluoperazine 5
10-20 35
TABLE-US-00002 TABLE 2 Recommended Oral Dosing of Atypical
Antipsychotics (mg) Atypical Starting Target Maximum Aripiprazole
10 15-30 30 Asenapine* 10 10-20 20 Clozapine 25 200-500 800
Iloperidone* 2 12-24 24 Olanzapine 5 10-20 30 Paliperidone 3 6-9 12
Quetiapine 100 400-800 1000 Risperidone 2 4-6 8.5 Ziprasidone 40
120-160 200 *Dosing as given in prescription package insert
[0033] For use in the methods and compositions described herein,
the antipsychotic drug (or a physiologically acceptable salt
thereof) may be a typical antipsychotic drug (i.e., first
generation antipsychotic drug). The typical antipsychotic drug may
be any one of fluphenazine, haloperidol, loxapine, molindone,
perphenazine, pimozide, sulpiride, thioridazine, or
trifluoperazine. In other embodiments, the antipsychotic drug (or a
physiologically acceptable salt thereof) may be an atypical
antipsychotic drug (i.e., second generation antipsychotic drug).
The atypical antipsychotic drug may be any one of aripiprazole,
asenapine, clozapine, iloperidone, olanzapine, paliperidone,
quetiapine, risperidone, or ziprasidone.
[0034] The only option for preventing TD is to avoid use of
antipsychotic drugs. When use of antipsychotic medication is
necessary, as in the treatment of schizophrenia, use of the
smallest possible dose of an antipsychotic for the shortest period
of time possible is a desirable option. However, neither option is
presently available for many schizophrenia patients who develop TD
and must continue to receive antipsychotic therapy. Treatments for
these TD patients involve changing or limiting the current
antipsychotic therapy. For example, a first generation drug may be
replaced with a second generation drug that has a lower risk of
causing TD. Switching to a lower dose of the agent causing TD may
also be helpful in alleviating symptoms. Changing drugs or lowering
the dose of a first generation antipsychotic must be made, however,
without exacerbating the underlying schizophrenia of the
subject.
[0035] As described herein, administering an antipsychotic drug in
combination with a VMAT2 inhibitor has a synergistic effect such
that less of the antipsychotic drug needs to be administered to
observe the same or similar efficacy than when the drug is
administered alone. VMAT2 inhibitors (and physiologically
acceptable salts thereof) may reduce the supply of monoamines in
the central nervous system by inhibiting the vesicular monoamine
transporter isoform 2 (VMAT2). Examples of VMAT2 inhibitors and
monoamine depletors that may be used in the methods described
herein include, for example, tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one, TBZ). TBZ is approved for the treatment of chorea
associated with Huntington's disease. Use of tetrabenazine for the
treatment of TD and a variety of hyperkinetic movement disorders
has also been described. Tetrabenazine is readily metabolized upon
administration to dihydrotetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-ol, DHTBZ), with the R,R,R stereoisomer of DHTBZ believed
to be the most active metabolite. In certain embodiments, the
methods described herein for treating a neuropsychiatric disorder
comprise administering an antipsychotic drug and
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (also called R,R,R-DHTBZ herein) or a
precursor thereof. Other VMAT2 inhibitors that may be used in the
methods and compositions described herein include TBZ analogs and
metabolites, reserpine, lobeline and analogs, and compounds
described in U.S. Pat. Nos. 8,039,627; 8,357,697; and 8,524,733. In
one embodiment, the VMAT2 inhibitor is (S)-2-Amino-3-methyl-butyric
acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester (see U.S. Pat. No. 8,039,627). In
another embodiment, the VMAT2 inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ). In still another embodiment,
the VMATs inhibitor is
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol (also called Compound 5-1
herein), or a precursor thereof (e.g., a prodrug of Compound 5-1).
In yet another embodiment, the VMAT2 inhibitor is tetrabenazine or
deuterated tetrabenazine. Deuterated tetrabenazine includes
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ). As described herein, any one of
the VMAT2 inhibitors may be combined with a pharmaceutically
acceptable excipient, carrier, and/or diluent to form a
pharmaceutical composition.
[0036] Characterizing the activity of a VMAT2 inhibitor can be
readily determined using in vitro methods and animal models
described in the art and herein (see, e.g., Teng, et al., J.
Neurochem. 71, 258-65, 1998; Near, (1986), Near, (1986), Mol.
Pharmacol. 30: 252-57). The capability of an antipsychotic drug to
have a desired therapeutic effect may be determined using in vivo
animal models, such as those described herein, which models are
familiar to persons skilled in the art. The conditioned avoidance
response (CAR) test has been shown to be an effective and reliable
preclinical model for assessing the antipsychotic activity of
compounds. Numerous studies have shown that typical and atypical
antipsychotic drugs selectively suppress CAR, thus making it an
ideal assay to screen for and to characterize antipsychotic
compounds (see, e.g., Wadenberg et al., Biobehav. Rev. 1999,
23:851-62).
[0037] Persons skilled in the art readily appreciate that such
assays and techniques are performed using appropriate negative
controls (e.g., vehicle only, diluent only, etc.) and appropriate
positive controls. Conditions for a particular in vitro assay
include temperature, buffers (including salts, cations, media), and
other components, which maintain the integrity of the test agent
and reagents used in the assay, and which are familiar to a person
skilled in the art and/or which can be readily determined.
Determining the effectiveness of an antipsychotic drug in an animal
model is typically performed using one or more statistical analyses
with which a skilled person will be familiar. By way of example,
statistical analyses such as two-way analysis of variance (ANOVA),
Fisher's exact test, and/or Bonferroni Test, may be used for
determining the statistical significance of differences between
animal groups.
[0038] Compounds described herein include all polymorphs, prodrugs,
isomers (including optical, geometric and tautomeric), salts,
solvates and isotopes thereof. With regard to stereoisomers, the
antipsychotics and VMAT2 inhibitors may have chiral centers and may
occur as racemates, racemic mixtures and as individual enantiomers
or diastereomers. All such isomeric forms are included, including
mixtures thereof. Unless specifically indicated, throughout the
specification and the appended claims, a given chemical formula or
name shall encompass tautomers and all stereo, optical and
geometrical isomers (e.g., enantiomers, diastereomers, E/Z isomers,
etc.) and racemates thereof as well as mixtures in different
proportions of the separate enantiomers, mixtures of diastereomers,
or mixtures of any of the foregoing forms when such isomers and
enantiomers exist, as well as salts thereof, including
pharmaceutically acceptable salts thereof and solvates thereof such
as for instance hydrates including solvates of the free compounds
or solvates of a salt of the compound.
[0039] As used herein, pharmaceutically (or physiologically)
acceptable salts refer to derivatives of the described compounds
wherein the parent compound is modified by making acid or base
salts thereof. Examples of pharmaceutically acceptable salts
include, but are not limited to, mineral or organic acid salts of
basic residues such as amines; alkali or organic salts of acidic
residues such as carboxylic acids; and the like. For example, such
salts include acetates, ascorbates, benzenesulfonates, benzoates,
besylates, bicarbonates, bitartrates, bromides/hydrobromides,
Ca-edetates/edetates, camsylates, carbonates,
chlorides/hydrochlorides, citrates, edisylates, ethane
disulfonates, estolates esylates, fumarates, gluceptates,
gluconates, glutamates, glycolates, glycollylarsnilates,
hexylresorcinates, hydrabamines, hydroxymaleates,
hydroxynaphthoates, iodides, isothionates, lactates, lactobionates,
malates, maleates, mandelates, methanesulfonates, mesylates,
methylbromides, methylnitrates, methylsulfates, mucates,
napsylates, nitrates, oxalates, pamoates, pantothenates,
phenylacetates, phosphates/diphosphates, polygalacturonates,
propionates, salicylates, stearates subacetates, succinates,
sulfamides, sulfates, tannates, tartrates, teoclates,
toluenesulfonates, triethiodides, ammonium, benzathines,
chloroprocaines, cholines, diethanolamines, ethylenediamines,
meglumines and procaines. Further pharmaceutically acceptable salts
can be formed with cations from metals like aluminium, calcium,
lithium, magnesium, potassium, sodium, zinc and the like. (see
also, e.g., Pharmaceutical Salts, Birge, S. M. et al., J. Pharm.
Sci., (1977), 66, 1-19).
[0040] In addition, prodrugs are also included with respect to the
compounds described herein. Prodrugs are any covalently bonded
carriers that release a compound in vivo when such prodrug is
administered to a patient. Prodrugs are generally prepared by
modifying functional groups in a way such that the modification is
cleaved, either by routine manipulation or in vivo, yielding the
parent compound. Prodrugs include, for example, compounds as
described herein wherein hydroxy, amine, or acid groups are bonded
to any group that, when administered to a subject, cleaves to form
the hydroxy, amine or acid groups. Thus, representative examples of
prodrugs include (but are not limited to) acetate, formate and
benzoate derivatives of alcohol and amine functional groups of a
compound. Further, in the case of a carboxylic acid (--COOH),
esters may be employed, such as methyl esters, ethyl esters, and
the like.
[0041] The compounds described herein may exist in a continuum of
solid states ranging from fully amorphous to fully crystalline.
Furthermore, some of the crystalline forms of the compounds may
exist as polymorphs. In addition, some compounds may also form
solvates with water or other organic solvents. The term solvate is
used herein to describe a molecular complex comprising a compound
and one or more pharmaceutically acceptable solvent molecules.
[0042] The compounds described herein in certain embodiments are
pharmaceutically acceptable isotopically labeled compounds wherein
one or more atoms are replaced by atoms having the same atomic
number but a different atomic mass. Examples include .sup.2H
(deuterium) and .sup.3H (tritium) for hydrogen, .sup.11C, .sup.13C
and .sup.14C for carbon, .sup.36Cl for chlorine, .sup.18F for
fluorine, .sup.123I and .sup.125I for iodine, .sup.13N and .sup.15N
for nitrogen, and .sup.35S for sulfur. Examples also include the
substitution of deuterium for .sup.1H, wherein the deuterium(s) are
selectively added to the molecule to alter the metabolism of the
drug resulting in some enhanced property such as an increased
half-life.
Methods of Treatment and Pharmaceutical Preparations and
Compositions
[0043] Provided herein are methods for treating any of the
disorders that are currently treated with antipsychotics, including
by way of non-limiting example, schizophrenia, schizoaffective
disorder, bipolar disorder, major depressive disorder (MDD), and
autism, manic depressive disorder, depression with psychotic
features, delusional disorder and other psychotic conditions, and
the symptoms of hallucinations and delusions. Methods are provided
herein for treating these disorders by administering to a subject
in need thereof a first generation (i.e., typical) or a second
generation (i.e., atypical) antipsychotic drug (e.g., a compound)
in combination with a VMAT2 inhibitor. In certain embodiments, when
the subject has developed a movement disorder (e.g., tardive
dyskinesia) or has at least one symptom of a movement disorder, the
methods comprising administering a VMAT2 inhibitor in combination
with the antipsychotic are useful for treating the movement
disorder (e.g., tardive dyskinesia). The VMAT2 inhibitor may
prevent (i.e., reduce likelihood of occurrence of), slow
progression of, delay, or treat a condition or disorder, such as a
movement disorder.
[0044] As disclosed herein, surprisingly and unexpectedly, when an
antipsychotic drug is administered in combination with a VMAT2
inhibitor for treating a neuropsychiatric disorder (e.g.,
schizophrenia, schizoaffective disorder, bipolar disorder, major
depressive disorder (MDD), and autism, manic depressive disorder,
depression with psychotic features, delusional disorder and other
psychotic conditions, and the symptoms of hallucinations and
delusions), the dose of the antipsychotic used in these methods is
lower than (i.e., reduced, decreased, less than) the
heretofore-described dosing range of the drug alone for effectively
treating these disorders. In certain embodiments, the dose of the
antipsychotic drug that is administered when combined with a VMAT2
inhibitor would not effectively treat the psychotic disorder if
administered alone (i.e., if administered in the absence of the
VMAT2 inhibitor). In other words, the combination of the VMAT2
inhibitor and the antipsychotic drug act synergistically in the
treatment of the disorder. When used in combination with a VMAT2
inhibitor, an antipsychotic drug may be used at a dose that if
administered alone would have little or no efficacy in treating the
neuropsychiatric disorder, that is, the dose of the antipsychotic
drug is subtherapeutic. That is, by combining a VMAT2 inhibitor
with a subtherapeutic dose of the antipsychotic drug, the efficacy
of the antipsychotic drug is enhanced. By way of example, treatment
of the neuropsychiatric disorder or symptoms thereof may provide
greater relief of anxiety and agitation when these are among the
symptoms of the disorder.
[0045] Decreasing the dose of an antipsychotic drug has the
beneficial effect of reducing the intensity of or preventing (i.e.,
decreasing the likelihood or risk of occurrence) one or more side
effects of the antipsychotic drug. In one embodiment, such as when
a typical antipsychotic drug is used for treating the disorder, the
likelihood of occurrence of tardive dyskinesia and other tardive
movement disorders may be reduced; the severity or intensity of the
movement disorder may be decreased or lessened; or the frequency of
occurrence of the movement disorder (or symptom thereof) may be
reduced (i.e., decreased, lessened). In another embodiment, such as
when an atypical drug is used in combination with a VMAT2 inhibitor
for treating a neuropsychiatric disorder or symptoms thereof, the
likelihood of occurrence or severity of a metabolic disturbance
such as weight gain, glucose intolerance, and risk of
atherosclerotic cardiovascular disease may be reduced. In other
embodiments, side effects that may be reduced by administering to a
subject in need thereof an antipsychotic (either an atypical or
typical antipsychotic) combined with a VMAT inhibitor include one
or more of sedation, dry mouth, sexual dysfunction, and cardiac
arrhythmias.
[0046] As understood by a person skilled in the medical art, the
terms, "treat" and "treatment," refer to medical management of a
disease, disorder, or condition of a subject (i.e., patient) (see,
e.g., Stedman's Medical Dictionary). The terms "treatment" and
"treating" embraces both preventative, i.e. prophylactic, or
therapeutic, i.e. curative and/or palliative, treatment. Thus the
terms "treatment" and "treating" comprise therapeutic treatment of
patients having already developed the condition, in particular in
manifest form. Therapeutic treatment may be symptomatic treatment
in order to relieve the symptoms of the specific indication or
causal treatment in order to reverse or partially reverse the
conditions of the indication or to stop or slow down progression of
the disease. Thus the compositions and methods described herein may
be used, for instance, as therapeutic treatment over a period of
time as well as for chronic therapy. In addition the terms
"treatment" and "treating" comprise prophylactic treatment, i.e., a
treatment of patients at risk to develop a condition mentioned
hereinbefore, thus reducing the risk.
[0047] The subject in need of the compositions and methods
described herein includes a subject who has been diagnosed by a
person skilled in the medical and psychiatric arts with a
neuropsychiatric disorder (e.g., schizophrenia, schizoaffective
disorder, bipolar disorder, major depressive disorder (MDD), or
autism). A subject (or patient) to be treated may be a mammal,
including a human or non-human primate. The mammal may be a
domesticated animal such as a cat or a dog.
[0048] Therapeutic and/or prophylactic benefit includes, for
example, an improved clinical outcome, both therapeutic treatment
and prophylactic or preventative measures, wherein the object is to
prevent or slow or retard (lessen) an undesired physiological
change or disorder, or to prevent or slow or retard (lessen) the
expansion or severity of such disorder. As discussed herein,
beneficial or desired clinical results from treating a subject
include, but are not limited to, abatement, lessening, or
alleviation of symptoms that result from or are associated the
disease, condition, or disorder to be treated; decreased occurrence
of symptoms; improved quality of life; longer disease-free status
(i.e., decreasing the likelihood or the propensity that a subject
will present symptoms on the basis of which a diagnosis of a
disease is made); diminishment of extent of disease; stabilized
(i.e., not worsening) state of disease; delay or slowing of disease
progression; amelioration or palliation of the disease state; and
remission (whether partial or total), whether detectable or
undetectable; and/or overall survival. "Treatment" can also mean
prolonging survival when compared to expected survival if a subject
were not receiving treatment. Subjects in need of treatment include
those who already have the condition or disorder as well as
subjects prone to have or at risk of developing the disease,
condition, or disorder (e.g., schizophrenia or schizoaffective
disorder, TD, or other conditions or disorders described herein),
and those in which the disease, condition, or disorder is to be
prevented (i.e., decreasing the likelihood of occurrence of the
disease, disorder, or condition).
[0049] A "therapeutically effective amount" generally refers to an
amount of a treatment, such as a antipsychotic drug and VMAT2
inhibitor, that (i) treats or prevents the particular disease or
condition, (ii) attenuates, ameliorates, or eliminates one or more
symptoms of the particular disease or condition, or (iii) prevents
or delays the onset of one or more symptoms of the particular
disease or condition described herein. Optimal doses may generally
be determined using experimental models and/or clinical trials. The
optimal dose may depend upon the body mass, weight, or blood volume
of the subject. In general, the dose range of a compound that is a
VMAT2 inhibitor applicable per day is usually from 5.0 to 150 mg
per day, and in certain embodiments from 10 to 100 mg per day. The
dose of the VMAT2 inhibitor included in a composition or
preparation also comprising an antipsychotic is sufficient to treat
a movement disorder such as TD (i.e., the dose is a therapeutically
effective dose for treating, preventing (i.e., reducing likelihood
of occurrence of), slow progression of, delay the movement
disorder).
[0050] Each of the antipsychotic and the VMAT2 inhibitor are
administered at a time and frequency appropriate for treating a
neuropsychiatric disorder, and the VMAT2 inhibitor is administered
in a manner appropriate at a time and frequency appropriate for
treating or preventing a movement disorder. The VMAT2 inhibitor may
be administered 1, 2, or 3 times a day. The antipsychotic drug may
be administered 1, 2, or 3 times a day independently or together
with the VMAT2 inhibitor. In other embodiments, the antipsychotic
is administered every week, every two weeks (approximately 2 times
per month), every three weeks, every four weeks (approximately once
per month), every 6 weeks, or every 8 weeks.
[0051] The oral dose range of typical and atypical antipsychotics
applicable per day when administered alone (i.e., in the absence of
a VMAT2 inhibitor) for an adult male schizophrenic subject may be
found in Tables 1 and 2, in product inserts, or in the art. These
recommended doses are sometimes augmented or reduced for different
patient populations, such as for women, the elderly, children and
adolescents, and for treatment of diseases other than
schizophrenia. A person skilled in the medical/psychiatric art
would therefore appreciate that the dose of a typical antipsychotic
or an atypical antipsychotic that provides therapeutic benefit to a
subject may need to be adjusted compared with the amounts shown in
Table 1 and Table 2 depending on the gender, health status, age,
presence of other medical or psychiatric conditions or diseases,
route of administration, and formulation of drug administered, and
other factors apparent to a person skilled in the art. As described
herein, the dose of an antipsychotic drug administered to a subject
may be significantly reduced compared with the amounts indicated in
Table 1 and Table 2 and as described in the art when the
antipsychotic drug is administered in combination with a VMAT2
inhibitor. The reduction in dose of the antipsychotic drug that is
required to have antipsychotic therapeutic benefit when the drug is
administered in combination with a VMAT2 inhibitor, (i.e., the
subtherapeutic dose of the antipsychotic drug), may be adjusted as
appropriate for a particular subject (e.g., gender, health status,
age, presence of other medical or psychiatric conditions or
diseases, route of administration, and formulation of drug
administered, and other factors apparent to a person skilled in the
art). In particular embodiments, the dose of the antipsychotic drug
used in combination with a VMAT2 inhibitor may be at least about
10% less, at least about 20% less, at least about 25% less, at
least about 30% less, at least about 35% less, at least about 40%
less, at least about 45% less, at least about 50% less, at least
about 55% less, at least about 60% less, at least about 65% less,
at least about 70% less, at least about 75% less, at least about
80% less, at least about 85% less, or at least about 90% less than
when used alone. In other certain embodiments, the dose of the
antipsychotic drug when used in combination with a VMAT2 inhibitor
may be between 10-90% less, 10-20% less, 10-25% less, 20-30% less,
25%-30% less, 25%-40% less, 25%-50% less, 25%-60% less, 25%-75%
less, 25%-80% less, 30-40% less, 30-60% less, 40-50% less, 40-60%
less, 50-60% less, 50-75% less, 60-70% less, 60-75% less, 70%-80%
less, or 80-90% less than when the antipsychotic drug is used
alone. As described herein examples of typical (i.e., first
generation) antipsychotic drugs include fluphenazine, haloperidol,
loxapine, molindone, perphenazine, pimozide, sulpiride,
thioridazine, and trifluoperazine. Examples of atypical (i.e.,
second generation antipsychotic drugs include aripiprazole,
asenapine, clozapine, iloperidone, olanzapine, paliperidone,
quetiapine, risperidone, and ziprasidone.
[0052] The minimum dose that is sufficient to provide effective
therapy and minimize toxicity is usually preferred. Subjects may
generally be monitored for therapeutic effectiveness by clinical
evaluation and psychiatric evaluation and by using assays suitable
for the condition being treated or prevented, which assays will be
familiar to those having ordinary skill in the art and are
described herein. The level of a compound that is administered to a
subject may be monitored by determining the level of the compound
in a biological fluid, for example, in the blood, blood fraction
(e.g., serum), and/or in the urine, and/or other biological sample
from the subject. Any method practiced in the art to detect the
compound may be used to measure the level of compound during the
course of a therapeutic regimen.
[0053] The dose of a composition comprising an antipsychotic and a
VMAT2 inhibitor described herein for treating schizophrenia or
schizoaffective disorder or a related disease or disorder, TD, or
other conditions or disorders described herein may depend upon the
subject's condition, that is, stage of the disease, severity of
symptoms caused by the disease, general health status, as well as
age, gender, and weight, and other factors apparent to a person
skilled in the medical and psychiatric arts. Similarly, the dose of
each compound may be determined according to parameters understood
by a person skilled in the art and as described herein.
[0054] The pharmaceutical compositions described herein that
comprise at least one of the antipsychotic compounds and the
pharmaceutical compositions described herein that comprise at least
one of the VMAT2 inhibitor compounds described herein may be
administered to a subject in need by any one of several routes that
effectively deliver an effective amount of each compound. Such
administrative routes include, for example, oral, parenteral,
enteral, rectal, intranasal, buccal, sublingual, intramuscular, and
transdermal. Compositions administered by these routes of
administration and others are described in greater detail herein.
The antipsychotic and the VMAT2 inhibitor when not formulated in
the same composition may be administered by the same or different
routes.
[0055] In certain embodiments, the VMAT2 inhibitor (or a
physiologically or pharmaceutically acceptable salt thereof) and
the antipsychotic drug (or a physiologically or pharmaceutically
acceptable salt thereof) are formulated together to form a single
composition. In other embodiments, a pharmaceutical preparation,
referred to herein, comprises a pharmaceutical composition
comprising an antipsychotic drug and a pharmaceutical composition
comprising a VMAT2 inhibitor and, which may be referred to herein
for convenience as a first pharmaceutical composition and a second
pharmaceutical composition. The pharmaceutical compositions of the
preparation may be administered concurrently or sequentially in
either order to a subject to provide the desired therapeutic
effect(s). As noted, reference to a "first pharmaceutical
composition" and "a second pharmaceutical composition" is for
convenience only and is not intended to describe an order of
administration.
[0056] Each pharmaceutical preparation and pharmaceutical
composition may further comprise at least one physiologically (or
pharmaceutically) acceptable or suitable excipient. Any
physiologically or pharmaceutically suitable excipient or carrier
(i.e., a non-toxic material that does not interfere with the
activity of the active ingredient(s)) known to those of ordinary
skill in the art for use in pharmaceutical compositions may be
employed in the compositions and preparations described herein.
Exemplary excipients include diluents and carriers that maintain
stability and integrity of the respective compound.
[0057] Pharmaceutically acceptable excipients are well known in the
pharmaceutical art and described, for example, in Rowe et al.,
Handbook of Pharmaceutical Excipients: A Comprehensive Guide to
Uses, Properties, and Safety, 5.sup.th Ed., 2006, and in Remington:
The Science and Practice of Pharmacy (Gennaro, 21.sup.st Ed. Mack
Pub. Co., Easton, Pa. (2005)). Exemplary pharmaceutically
acceptable excipients include sterile saline and phosphate buffered
saline at physiological pH. Preservatives, stabilizers, dyes,
buffers, and the like may be provided in the pharmaceutical
composition. In addition, antioxidants and suspending agents may
also be used.
[0058] The pharmaceutical compositions may be in the form of a
solution. The solution may comprise saline or sterile water, and
may optionally include antioxidants, buffers, bacteriostats, and
other common additives. Alternatively, they may be in the form of a
solid, such as powder, tablets, pills, or the like. A composition
comprising any one of the compounds described herein may be
formulated for sustained or slow release. Such compositions may
generally be prepared using well known technology and administered
by, for example, oral, rectal or subcutaneous implantation, or by
implantation at the desired target site. Sustained-release
formulations may contain the compound dispersed in a carrier matrix
and/or contained within a reservoir surrounded by a rate
controlling membrane. Excipients for use within such formulations
are biocompatible, and may also be biodegradable; preferably the
formulation provides a relatively constant level of active
component release. The amount of active compound contained within a
sustained release formulation depends upon the site of
implantation, the rate and expected duration of release, and the
nature of the condition to be treated or prevented.
[0059] For oral formulations, at least one of the compounds
described herein can be used alone or in combination with
appropriate additives to make tablets, powders, granules or
capsules, for example, dispersing and surface active agents; with
conventional additives, such as lactose, mannitol, corn starch or
potato starch; with binders; with disintegrators; with lubricants;
and if desired, with diluents, buffering agents, moistening agents,
preservatives, coloring agents, and flavoring agents. Compounds may
be formulated with a buffering agent to provide for protection of
the compound from low pH of the gastric environment and/or an
enteric coating. A compound included in the compositions may be
formulated for oral delivery with a flavoring agent, e.g., in a
liquid, solid or semi-solid formulation and/or with an enteric
coating. Oral formulations may be provided as gelatin capsules,
which may contain the active compound along with powdered carriers,
such as lactose, starch, cellulose derivatives, magnesium stearate,
stearic acid, and the like. Similar carriers and diluents may be
used to make compressed tablets.
[0060] Antipsychotics are commonly administered as oral
compositions that are administered once or twice daily. Fast
disintegrating buccal tablets and single dose injections have also
been marketed. Long-acting medication has potential advantages over
daily medication in improving compliance, thus reducing
hospitalization and relapse rates. Intramuscular depot injections,
generally providing 2 or 4 weeks of sustained efficacy, are
available for aripiprazole, fluphenazine, haloperidol, olanzapine,
paliperidone and risperidone. By way of non-limiting example, the
antipsychotic drug can be administered according to the methods
described herein on a once-daily or twice-daily schedule (or more
if desired), typically with a single pill given each time. In
certain specific embodiments, the antipsychotic drug and the VMAT2
inhibitor are formulated in the same tablet or pill for oral
administration. In other embodiments, each of the VMAT2 inhibitor
and the antipsychotic drug are formulated separately into different
pills or tablets.
[0061] Also provided are kits that comprise one or more unit doses
of each of the VMAT2 inhibitor and the antipsychotic drug. A
non-limiting example of such a kit includes a blister pack. In
another embodiment, the antipsychotic may be administered as a
long-acting intramuscular injection and the VMAT2 inhibitor may be
administered on a separate daily schedule.
[0062] In other embodiments, a preparation or composition that
comprises an antipsychotic drug and a VMAT2 inhibitor further
comprises one or more additional therapeutic agents, particularly
therapeutic agents suitable for the treatment and/or prevention of
the neuropsychiatric conditions and diseases described herein.
Subjects who have a neuropsychiatric disorder, such as depression
or bipolar disorder, are treated with multiple therapeutic drugs to
control the symptoms of the disease. Additional therapeutic agents
that may be suitable for combination with an antipsychotic drug and
a VMAT2 inhibitor include, for example, one or more drugs useful
for treating depression, bipolar disorder, or other disorders.
Accordingly, in certain embodiments, a pharmaceutical preparation
is provided that comprises an antipsychotic drug, or a
physiologically acceptable salt thereof, and a VMAT2 inhibitor, or
a physiologically acceptable salt thereof, and one or more
additional therapeutic agents, optionally together with one or more
pharmaceutically acceptable excipients, carriers, and/or
diluents.
[0063] The following examples are provided for purposes of
illustration, not limitation.
EXAMPLES
Example 1
Conditioned Avoidance Response Assay of Antipsychotic Activity for
Risperidone, R,R,R-DHTBZ, and a Combination Thereof
[0064] The conditioned avoidance response (CAR) test has been shown
to be an effective and reliable preclinical model for assessing the
antipsychotic activity of compounds. In the CAR paradigm, a rat is
trained in a two chamber shuttle box to respond to a conditioned
stimulus (auditory) by negative reinforcement. If the animal fails
to move to the other chamber upon presentation of an auditory
stimulus, a mild foot shock is applied to the side where the rat is
located. The rat learns to avoid the mild foot shock by moving to
the other chamber upon initiation of the auditory signal, termed a
conditioned avoidance response. Crossing to the other chamber
during administration of the shock is termed an escape response. If
a rat fails to move to the other chamber even upon administration
of the foot shock, the rat is considered to have an escape failure.
Numerous studies have shown that typical and atypical antipsychotic
drugs selectively suppress CAR, thus making it an ideal assay to
screen potential antipsychotic compounds (see, e.g., Wadenberg et
al., Biobehav. Rev. 1999. 23: 851-62).
[0065] Rats were trained every day for 3 to 4 weeks. In the
training session, rats were placed in the CAR two-way shuttle box
and the training period of 20 trials ensued. A trial consisted of a
10-sec presentation of an 80 dB white noise followed by a scrambled
0.6 mA foot shock lasting up to 20 sec. The inter-trial interval
ranged from 20-60 sec. The rat learned to avoid shock by moving
from one compartment to the other when the conditioned stimulus was
presented (a conditioned avoidance response). A rat was deemed
sufficiently trained if it avoided the shock when presented with
the conditioned stimulus at least 19 times out of the 20 trials.
Rats that did not pass these criteria were not used.
[0066] On test day, trained animals were acclimated in the test
room for 30 minutes prior to testing. They were then dosed with
compound and placed in the CAR two-way shuttle box. In the test, 20
trials were performed on each rat. In each trial the conditioned
stimulus was applied (10-sec presentation of 80 dB white noise),
followed by the foot shock (a scrambled 0.6 mA foot shock lasting
up to 20 sec). If the animal moved to the other chamber on
presentation of the conditioned stimulus, it was scored as a
conditioned avoidance response. If it moved upon presentation of
the foot shock, it was scored as an escape. If it failed to move
upon presentation of the foot shock, it was scored as an escape
failure. Antipsychotic efficacy is evident by an increase in the
number of escapes. Data were analyzed by analysis of variance
(ANOVA) followed by post-hoc comparisons with the Bonferroni Test
when appropriate. An effect is considered significant if p<0.05.
Outliers defined as two standard deviations above or below the mean
were detected and were removed from all analysis. Results are
reported as mean.+-.SEM for number of escapes.
[0067] Animals:
[0068] Male Wistar rats from Harlan (Indianapolis, Ind.) were used
in the study. Upon receipt, rats were assigned unique
identification numbers and were group housed with 3 rats per cage
in polycarbonate cages with micro-isolator filter tops. All rats
were examined, handled, and weighed prior to initiation of the
study to assure adequate health and suitability. The rats were
maintained at 12/12 light/dark cycle with lights on at 6:00 A.M.
The room temperature was maintained between 20 and 23.degree. C.
with a relative humidity maintained around 50%. Chow and water were
provided ad libitum for the duration of the study. For each test,
animals were randomly assigned across treatment groups. Each
treatment group contained 10 animals at time of testing.
[0069] Test Compounds:
[0070] Risperidone (0.03, 0.1, 0.3, and 1 mg/kg) was dissolved in
10% DMSO and administered i.p. 30 min prior to testing at a dose
volume of 1 ml/kg.
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H--
pyrido[2,1-a]isoquinolin-2-ol (called R,R,R-DHTBZ herein) (0.15
mg/kg) was dissolved in sterile water and administered p.o. 30 min
prior to testing at a dose volume of 1 ml/kg and immediately
following administration of risperidone or its vehicle. The vehicle
control group received 10% DMSO (i.p., 30 min pretreatment, 1
ml/kg) immediately followed by administration of sterile water
(p.o., 1 ml/kg).
[0071] Results:
[0072] Risperidone (0.1 mg/kg) or R,R,R-DHTBZ (0.15 mg/kg)
administered singly did not increase the number of escapes compared
to vehicle. A combination of risperidone (0.1 mg/kg) and
R,R,R-DHTBZ (0.15 mg/kg), at doses that showed no efficacy when
administered alone, significantly increased the number of escapes
compared to vehicle. This finding strongly suggests a synergistic
effect on antipsychotic activity when the combination of
risperidone and R,R,R-DHTBZ is administered.
[0073] Additionally, adding R,R,R-DHTBZ (0.15 mg/kg) to risperidone
reduced the amount of risperidone needed to produce an
antipsychotic effect. Administered singly, a dose of 0.3 mg/kg of
risperidone was necessary to show antipsychotic efficacy in the CAR
paradigm. The addition of R,R,R-DHTBZ (0.15 mg/kg) to risperidone
resulted in antipsychotic activity at much lower doses of
risperidone (0.03 and 0.1 mg/kg). This finding strongly suggests a
synergistic effect of the combination on antipsychotic
activity.
[0074] FIG. 1 shows a graph expressing the statistically
significant marked increase of escapes in the conditioned avoidance
test for the combination of the antipsychotic risperidone (0.1
mg/kg) in combination with the VMAT2 inhibitor R,R,R-DHTBZ (0.15
mg/kg). Risperidone (0.1 mg/kg) and R,R,R-DHTBZ (0.15 mg/kg)
administered singly at the same doses as used in the combination
did not result in changes from the vehicle results. These findings
are strongly suggestive of antipsychotic activity due to the
synergy of the combination.
[0075] FIG. 2 shows a shift in ED.sub.50 value for risperidone
(0.03, 0.1, 0.3 and 1.0 mg/kg) dosed in combination with
R,R,R-DHTBZ (0.15 mg/kg) as compared to risperidone (0.03, 0.1, 0.3
and 1.0 mg/kg) administered singly in rats. The ED.sub.50 value of
risperidone calculated from the fitted curve as the dose of
compound required to produce 50% of the maximum response to
compound decreased approximately 6-fold from a value of 0.14 mg/kg
when risperidone was administered singly to 0.024 mg/kg when
risperidone was administered in combination with the VMAT2
inhibitor R,R,R-DHTBZ (0.15 mg/kg). This 6-fold reduction
corresponds to an 83% reduction of the risperidone dose. R,R,R
DHTBZ (0.15 mg/kg) was similar to vehicle and showed no
antipsychotic activity when administered singly. These findings are
strongly suggestive of antipsychotic activity due to the synergy of
the combination.
Example 2
Conditioned Avoidance Response Assay of Antipsychotic Activity for
Olanzapine, R,R,R-DHTBZ, and a Combination Thereof
[0076] The protocol of Example 1 was conducted with the atypical
antipsychotic olanzapine and R,R,R-DHTBZ.
[0077] Test Compounds:
[0078] Olanzapine (0.3, 0.6, 1 and 3 mg/kg) was dissolved in 10%
DMSO and administered i.p. 30 min prior to testing at a dose volume
of 1 ml/kg.
[0079] R,R,R-DHTBZ (0.15 mg/kg) was dissolved in sterile water and
administered p.o. 30 min prior to testing at a dose volume of 1
ml/kg and immediately following administration of olanzapine or its
vehicle. 10% DMSO (i.p., 30 min pretreatment, 1 ml/kg) immediately
followed by administration of sterile water (p.o., 1 ml/kg) was
used as the vehicle control group.
[0080] Results:
[0081] Olanzapine (0.6 mg/kg) or R,R,R-DHTBZ (0.15 mg/kg)
administered singly did not increase the number of escapes compared
to vehicle. A combination of olanzapine (0.6 mg/kg) and R,R,R-DHTBZ
(0.15 mg/kg), at doses which showed no efficacy when administered
alone, significantly increased the number of escapes compared to
vehicle. This finding strongly suggests a synergistic effect on
antipsychotic activity due to the combination.
[0082] Additionally, adding R,R,R-DHTBZ (0.15 mg/kg) to olanzapine
reduced the amount of olanzapine needed to produce an antipsychotic
effect. Administered singly, a dose of 1 mg/kg of olanzapine was
necessary to show antipsychotic efficacy in the CAR paradigm. The
addition of R,R,R-DHTBZ (0.15 mg/kg) to olanzapine resulted in
antipsychotic activity at a lower dose of olanzapine (0.6 mg/kg).
This finding strongly suggests a synergistic effect on
antipsychotic activity due to the combination.
[0083] FIG. 3 shows a graph expressing the statistically
significant marked increase of escapes in the conditioned avoidance
test for the combination of the antipsychotic olanzapine (0.6
mg/kg) in combination with the VMAT2 inhibitor R,R,R-DHTBZ (0.15
mg/kg). Olanzapine (0.6 mg/kg) and R,R,R-DHTBZ (0.15 mg/kg)
administered singly at the same doses as used in the combination
did not result in changes from the vehicle results. These findings
are strongly suggestive of antipsychotic activity due to the
synergy of the combination.
[0084] FIG. 4 shows a shift in ED.sub.50 value for olanzapine (0.3,
0.6, 1.0, and 3.0 mg/kg) dosed in combination with R,R,R-DHTBZ
(0.15 mg/kg) as compared to olanzapine (0.3, 0.6, 1.0, and 3.0
mg/kg) administered singly in rats. The ED.sub.50 value of
olanzapine calculated from the fitted curve as the dose of compound
required to produce 50% of the maximum response to compound
decreased approximately 2-fold from a value of 1.2 mg/kg when
olanzapine was administered singly to 0.51 mg/kg when olanzapine
was administered in combination with the VMAT2 inhibitor
R,R,R-DHTBZ (0.15 mg/kg). This 2-fold reduction corresponds to a
50% reduction of the olanzapine dose. These findings are strongly
suggestive of antipsychotic activity due to the synergy of the
combination.
Example 3
Conditioned Avoidance Response Assay of Antipsychotic Activity for
Risperidone, Compound 5-1, and a Combination Thereof
[0085] The protocol of Example 1 was conducted with the atypical
antipsychotic risperidone and Compound 5-1 (see Example 5 for
synthesis of Compound 5-1).
[0086] Test Compounds:
[0087] Risperidone (0.1, 0.3, and 1 mg/kg) was dissolved in 10%
DMSO and administered i.p. 30 min prior to testing at a dose volume
of 1 ml/kg. Compound 5-1 (0.3 mg/kg) was dissolved in sterile water
and administered p.o. 60 min prior to testing at a dose volume of 1
ml/kg, that is, 30 min before administration of risperidone or its
vehicle. The vehicle control group received sterile water (p.o., 1
ml/kg) followed 30 min later by administration of 10% DMSO (i.p.,
30 min pretreatment, 1 ml/kg).
[0088] Results:
[0089] Risperidone (0.1 mg/kg) or Compound 5-1 (0.3 mg/kg)
administered singly did not increase the number of escapes compared
to vehicle. A combination of risperidone (0.1 mg/kg) and Compound
5-1 (0.3 mg/kg), at doses which showed no efficacy when
administered alone, significantly increased the number of escapes
compared to vehicle. This finding strongly suggests a synergistic
effect on antipsychotic activity when the combination of
risperidone and Compound 5-1 is administered.
[0090] Additionally, adding Compound 5-1 (0.3 mg/kg) to risperidone
reduced the amount of risperidone needed to produce an
antipsychotic effect. Administered singly, a dose of 0.3 mg/kg of
risperidone was necessary to show antipsychotic efficacy in the CAR
paradigm. The addition of Compound 5-1 (0.3 mg/kg) to risperidone
resulted in antipsychotic activity at much lower doses of
risperidone (0.1 mg/kg). This finding strongly suggests a
synergistic effect of the combination on antipsychotic
activity.
[0091] FIG. 5 shows a graph expressing the statistically
significant marked increase of escapes in the conditioned avoidance
test for the combination of the antipsychotic risperidone (0.1
mg/kg) in combination with the VMAT2 inhibitor Compound 5-1 (0.3
mg/kg). Risperidone (0.1 mg/kg) and Compound 5-1 (0.3 mg/kg)
administered singly at the same doses as used in the combination
did not result in changes from the vehicle results. These findings
are strongly suggestive of antipsychotic activity due to the
synergy of the combination.
[0092] FIG. 6 shows a shift in the dose of risperidone (0.1 mg/kg)
dosed in combination with Compound 5-1 (0.3 mg/kg) as compared to
risperidone (0.1, 0.3 and 1.0 mg/kg) administered singly in rats.
The dose of risperidone calculated from the fitted line as the dose
of compound required to produce 8 escapes decreased approximately
7-fold from a value of 0.26 mg/kg when risperidone was administered
singly to 0.038 mg/kg when risperidone was administered in
combination with the VMAT2 inhibitor, Compound 5-1 (0.3 mg/kg).
This 7-fold reduction corresponds to an 86% reduction of the
risperidone dose. Compound 5-1 (0.3 mg/kg) was similar to vehicle
and showed no antipsychotic activity when administered singly.
These findings are strongly suggestive of antipsychotic activity
due to the synergy of the combination.
Example 4
Conditioned Avoidance Response Assay of Antipsychotic Activity for
Olanzapine, Compound 5-1, and a Combination Thereof
[0093] The protocol described in Examples 1 and 2 was conducted
with the atypical antipsychotic olanzapine and Compound 5-1 (see
Example 5).
[0094] Test Compounds:
[0095] Olanzapine (0.6, 1 and 3 mg/kg) was dissolved in 10% DMSO
and administered i.p. 30 min prior to testing at a dose volume of 1
ml/kg. Compound 5-1 (0.3 mg/kg) was dissolved in sterile water and
administered p.o. 60 min prior to testing at a dose volume of 1
ml/kg, that is, 30 min before administration of olanzapine or its
vehicle. The vehicle control group received sterile water (p.o., 1
ml/kg) followed 30 min later by administration of 10% DMSO (i.p.,
30 min pretreatment, 1 ml/kg).
[0096] Results:
[0097] Olanzapine (0.6 mg/kg) or Compound 5-1 (0.3 mg/kg)
administered singly did not increase the number of escapes compared
to vehicle. A combination of olanzapine (0.6 mg/kg) and Compound
5-1 (0.3 mg/kg), at doses which showed no efficacy when
administered alone, significantly increased the number of escapes
compared to vehicle. This finding strongly suggests a synergistic
effect on antipsychotic activity when the combination of olanzapine
and Compound 5-1 is administered.
[0098] Additionally, combining Compound 5-1 (0.3 mg/kg) with
olanzapine reduced the amount of olanzapine needed to produce an
antipsychotic effect. Administered singly, a dose of 1 mg/kg of
olanzapine was necessary to show antipsychotic efficacy in the CAR
paradigm. The addition of Compound 5-1 (0.3 mg/kg) to olanzapine
resulted in antipsychotic activity at a lower dose of olanzapine
(0.6 mg/kg). This finding strongly suggests a synergistic effect of
the combination on antipsychotic activity.
[0099] FIG. 7 shows a graph expressing the statistically
significant marked increase of escapes in the conditioned avoidance
test for the combination of the antipsychotic olanzapine (0.6
mg/kg) in combination with the VMAT2 inhibitor, Compound 5-1 (0.3
mg/kg). Olanzapine (0.6 mg/kg) and Compound 5-1 (0.3 mg/kg)
administered singly at the same doses as used in the combination
did not result in changes from the vehicle results. These findings
are strongly suggestive of antipsychotic activity due to the
synergy of the combination.
[0100] FIG. 8 shows a shift in the dose of olanzapine (0.6 mg/kg)
dosed in combination with Compound 5-1 (0.3 mg/kg) as compared to
olanzapine (0.6, 1 and 3 mg/kg) administered singly in rats. The
dose of olanzapine calculated from the fitted line as the dose of
compound required to produce 5 escapes decreased approximately
7-fold from a value of 0.93 mg/kg when olanzapine was administered
singly to 0.18 mg/kg when olanzapine was administered in
combination with the VMAT2 inhibitor Compound 5-1 (0.3 mg/kg). This
5-fold reduction corresponds to an 80% reduction of the olanzapine
dose. Compound 5-1 (0.3 mg/kg) was similar to vehicle and showed no
antipsychotic activity when administered singly. These findings are
strongly suggestive of antipsychotic activity due to the synergy of
the combination.
Example 5
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-pyr-
ido[2,1-a]isoquinolin-2-yl]methanol
##STR00001##
[0101] Step 5A:
(3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-pyrido-
[2,1-a]isoquinoline-2-carbonitrile
[0102] To a 3 L 3-neck round bottomed flask DMSO (1.1 L) and TOSMIC
(104 g, 532.5 mmol, 1.3 eq) were charged. To this mixture KO-t-Bu
(119.5 g, 1.065 mol) was charged at once at ambient temp
(22.degree. C.). An exotherm was observed and the temperature of
the mixture increased to 39.degree. C. Then a suspension of
tetrabenazine (130 g, 410 mmol) in DMSO (500 mL) was added to the
reaction mixture slowly over 25 min (a slight exotherm observed).
EtOH (10.5 mL) was added to this mixture, and the mixture was
stirred at ambient temp for 3 h. LC-MS analysis of the mixture
revealed presence of .about.4:1 ratio of 5a and starting material.
The mixture was poured into cold water (9 L). The mixture was then
extracted with EtAOc (4 L). The aqueous layer was extracted with
EtOAc (2 L). The combined organics were washed with brine (2 L),
dried over Na.sub.2SO.sub.4 and concentrated. The residue was
dissolved in acetone (200 ml) and loaded onto a silica column (2 Kg
silica gel, packed with hexanes). The column was eluted first with
hexanes (2.5 L), followed by 5-20% of acetone in hexanes. The
fractions containing 5a and other impurities were combined and
concentrated to give an orange oil (72 g), which was dissolved in
acetone (100 ml) and loaded onto a silica column (1 Kg silica gel,
packed with hexanes). The column was eluted first with hexanes (1
L), followed by 5% of acetone in hexanes (2 L), 10% of acetone in
hexanes (2 L), 15% of acetone in hexanes (2 L), and 20% of acetone
in hexanes (2 L). The fractions containing >90% purity were
combined and concentrated to give
(3S,11bR)-9,10-dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-pyrido-
[2,1-a]isoquinoline-2-carbonitrile 5a as an orange solid (61 g, m/z
329.2 [MH.sup.+]). The fractions containing a mixture of 5a and
starting material were collected and concentrated to give 48 g of
material, which was dissolved in DMSO (50 ml) and was added to a
mixture of TOSMIC (25 g) and KO-t-Bu (28.7 g) in DMSO (250 ml) as
shown above. The residue was dissolved in acetone (10 ml) and
loaded onto a silica column (600 g silica gel, packed with
hexanes). The column was eluted first with hexanes (800 ml),
followed by 5-20% of acetone in hexanes. The fractions containing
product were combined and concentrated to give orange solid 5a (33
g).
Step 5B:
(3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11b-
H-pyrido[2,1-a]isoquinoline-2-carboxylic acid
[0103] A 1 gallon pressure reactor was charged with a suspension of
5a (94 g, 286 mmol) in methanol (940 ml) and NaOH (343 g, 8.6 mol)
in water (940 ml). This mixture was stirred at 120.degree. C.
(internal temp) for 67 h. The mixture was cooled to room
temperature and transferred to a round bottom flask. The mixture
was concentrated in a rotavap to .about.1 L. The mixture was then
adjusted pH to 7 using aqueous 6N HCl under cooling. The mixture
was extracted with DCM (2.times.3 L and 1.times.2 L). The combined
organics were dried over Na.sub.2SO.sub.4 and concentrated to give
a dark residue (88 g). The dark residue was taken in acetonitrile
(500 ml) and stirred for 30 min. The mixture was filtered and the
solid was washed with acetonitrile (50 ml). The solid was dried
under vacuum for 2 hours to afford light brown solid (42 g, 49%).
This solid was combined with the filtrate and concentrated to a
residue. The residue was dissolved in DCM (150 ml) and loaded onto
a silica column packed with DCM. The column was eluted with 0-25%
of methanol in DCM. The fractions containing product were combined
and concentrated to give
(3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-pyrido-
[2,1-a]isoquinoline-2-carboxylic acid 5b as a pale brown solid (71
g, 71% yield, 92% purity, m/z 348.2 [MH.sup.+]).
Step 5C:
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H-
,11bH-pyrido[2,1-a]isoquinolin-2-yl]methanol
[0104] A 3 L round bottom flask was charged with 5b (73.5 g, 211.5
mmol) and THF (1.48 L). This mixture was stirred and cooled to
10.degree. C. (internal temp). To this mixture was added 1 M LAH in
THF (423 ml, 423 mmol) slowly over 20 min keeping the temp below
20.degree. C. The cooling bath was removed, and the mixture was
warmed up to room temp. The mixture was heated to 55.degree. C. and
stirred for 30 min. The mixture was cooled to room temp and then to
10.degree. C. EtOAc (30 ml) was added slowly to quench un-reacted
LAH followed by ethanol (30 ml). Then water (150 ml) was added to
this mixture. The mixture was then concentrated to remove most of
organic solvents. Then the mixture was diluted with water (700 ml)
and DCM (1 L). The suspension was filtered through a pad of celite.
The filtered cake was washed with DCM (2.times.500 ml). The
combined filtrates were taken in separatory funnel and the layers
separated. The aqueous layer was extracted with DCM (1 L). The
combined organics were dried over Na.sub.2SO.sub.4 and concentrated
to give a dark residue. The residue was chromatographed on silica
column using 0-10% of methanol in DCM as eluent. The fractions
containing product were combined and concentrated to afford foamy
orange residue. To this residue hexanes (100 ml) was added and
concentrated under reduced pressure at 45.degree. C. for 2 h to
afford
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol (5-1) (also called Compound
5-1 herein) as a pale brown solid (51 g, 72%, 95% HPLC purity by
220 nm, m/z 334.2 [MH.sup.+]). This material may be further
purified by silica gel chromatography using 0-10% of methanol in
DCM or ethyl acetate as eluent.
Step 5D: [(2R,3
S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-pyrido[2-
,1-a]isoquinolin-2-yl]methanol HCl salt
[0105] A 2 L roundbottom flask was charged with 5-1 (43 g, 129
mmol) and diethyl ether (860 mL). This mixture was stirred and
cooled to 15.degree. C. (internal temp). To this mixture was added
2 M HCl in diethyl ether (97 ml, 193 mmol) slowly over 15 min. A
white precipitate formed. The cooling bath was removed and the
mixture was warmed to room temp. The mixture was then stirred for
45 min. The mixture was filtered and the filtered solid was washed
with diethyl ether (100 ml), with MTBE (100 ml) and then with
hexanes (100 ml). The solid was then dried in vacuum oven at
40.degree. C. for 18 h.
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol HCl salt (5-1 HCl) was
isolated as an off-white solid (44.7 g, 94% yield, m/z 334.2
[MH.sup.+]).
Example 6
Methods for Determining Vmat2 Inhibitory Activity of a Compound
[0106] Examples of techniques for determining the capability of a
compound to inhibit VMAT2 are provided below.
[0107] The procedure is adapted from that described previously
(see, e.g., Near, (1986), Mol. Pharmacol. 30: 252-57; Teng, et al.,
J. Neurochem. 71, 258-65, 1998). Homogenates from human platelets
or Sprague-Dawley rat forebrain were prepared by homogenization and
then washed by centrifugation as described previously (see, e.g.,
Hoare et al., (2003) Peptides 24:1881-97). In a total volume of 0.2
mL in low-binding 96-well plates (Corning #3605), twelve
concentrations of Compound 5-1 and R,R,R-DHTBZ were competed
against 6 nM .sup.3H-dihydrotetrabenazine (American Radiolabeled
Chemicals, Kd 2.6 nM) on rat forebrain homogenate (100 .mu.g
membrane protein per well) or human platelet homogenate (50 .mu.g
membrane protein per well) in VMAT2 binding buffer (Dulbecco's
phosphate buffered saline, 1 mM EDTA, pH 7.4). Following incubation
at 25.degree. C. for two hours, bound radioligand was collected by
rapid filtration onto GF/B glass fiber filters using a Unifilter-96
Harvester (PerkinElmer). Filter plates were pre-treated for 10
minutes with 0.1% polyethylenimine, and following harvesting the
filter plates were washed with 800 .mu.l VMAT2 binding buffer.
Bound radioligand was quantified by scintillation counting using a
Topcount NXT (PerkinElmer). The results of the competition binding
studies are presented below in Table 3 and Table 4.
TABLE-US-00003 TABLE 3 Rat Forebrain VMAT2 Affinity from
Competition Binding Studies Compound pKi (n) Ki (nM) Compound 5-1
8.6 .+-. 0.1 (2) 2.6 R,R,R-DHTBZ 8.7 .+-. 0.2 (6) 1.9
TABLE-US-00004 TABLE 4 Human Platelet VMAT2 Affinity from
Competition Binding Studies Compound pKi (n) Ki (nM) Compound 5-1
8.3 .+-. 0.1 (2) 5.2 R,R,R-DHTBZ 8.6 .+-. 0.3 (3) 2.6
[0108] Another technique that may be routinely performed to
determine the capability of a compound to inhibit VMAT2 is provided
below. The following procedure is adapted from a previously
described method (see Teng, et al., J. Neurochem. 71, 258-65,
1998).
[0109] Preparation of rat striatal vesicles: Rat striata from three
rats are pooled and homogenized in 0.32 M sucrose. The homogenate
is then centrifuged at 2,000.times.g for 10 min at 4.degree. C. and
the resulting supernatant is centrifuged at 10,000.times.g for 30
min at 4.degree. C. The resulting pellet containing the enriched
synaptosomal fraction (2 mL) is subjected to osmotic shock by
addition of 7 mL of distilled H.sub.2O, and subsequently the
suspension is homogenized. The osmolarity is restored by the
addition of 0.9 mL of 0.25 M HEPES and 0.9 mL of 1.0 M neutral
L-(+)-tartaric acid dipotassium salt buffer (pH 7.5), followed by a
20 min centrifugation (20,000.times.g at 4.degree. C.). The
supernatant is then centrifuged for 60 min (55,000.times.g at
4.degree. C.) and the resulting supernatant is centrifuged for 45
min (100,000.times.g at 4.degree. C.). The resulting pellet is
resuspended in 25 mM HEPES, 100 mM L-(+)-tartaric acid dipotassium
salt, 5 mM MgCl.sub.2, 10 mM NaCl, 0.05 mM EGTA, pH 7.5 to a
protein concentration of 1-2 mg/mL and stored at -80.degree. C. for
up to 3 weeks without appreciable loss of binding activity.
Immediately before use, the final pellet is resuspended in binding
buffer (25 mM HEPES, 100 mM L-(+)-tartaric acid dipotassium salt, 5
mM MgCl.sub.2, 10 mM NaCl, 0.05 mM EGTA, 0.1 mM EDTA, 1.7 mM
ascorbic acid, pH 7.4).
[0110] [.sup.3H]-dihydrotetrabenazine (DHTBZ) Binding: Aliquots of
the vesicle suspension (0.16 mL, 15 .mu.g of protein/mL) are
incubated with competitor compounds (ranging from 10.sup.-6 to
10.sup.-12 M) and 2 nM [.sup.3H]-dihydrotetrabenazine (HTBZ;
specific activity: 20 Ci/mmol, American Radiolabeled Chemicals,
Inc.) for 1 h at room temperature in a total volume of 0.5 mL. The
reaction is terminated by rapid filtration of the samples onto
Whatman GF/F filters using a Brandel cell harvester. Nonspecific
binding is determined using 20 .mu.M tetrabenazine (TBZ). Filters
are previously soaked for 2 h with ice-cold polyethyleneimine
(0.5%). After the filters are washed three times with the ice-cold
buffer, they are placed into scintillation vials with 10 mL
scintillation cocktail. Bound radioactivity is determined by
scintillation spectrometry.
[0111] The various embodiments described herein can be combined to
provide further embodiments. Described herein are the following
exemplary embodiments.
Embodiment 1
[0112] A method for treating a neuropsychiatric disorder in a
subject comprising administering to the subject (a) an
antipsychotic drug and (b) a VMAT2 inhibitor, wherein the
therapeutically effective amount of the antipsychotic drug
administered to the subject is less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor.
Embodiment 2
[0113] The method of Embodiment 1 wherein the neuropsychiatric
disorder is schizophrenia, schizoaffective disorder, bipolar
disorder, major depressive disorder (MDD), or autism.
Embodiment 3
[0114] The method of Embodiment 1 or Embodiment 2 wherein the
antipsychotic drug and the VMAT2 inhibitor are administered
concurrently.
Embodiment 4
[0115] The method of any one of Embodiments 1-3 wherein the
antipsychotic drug and the VMAT2 inhibitor are formulated in the
same pharmaceutical composition.
Embodiment 5
[0116] The method of any one of Embodiments 1-3 wherein the
antipsychotic drug is formulated in a first pharmaceutical
composition and the VMAT2 inhibitor is formulated in a second
pharmaceutical composition.
Embodiment 6
[0117] The method of any one of Embodiments 1-5 wherein the
antipsychotic drug is a typical antipsychotic drug.
Embodiment 7
[0118] The method of Embodiment 6 wherein the typical antipsychotic
drug is fluphenazine, haloperidol, loxapine, molindone,
perphenazine, pimozide, sulpiride, thioridazine, or
trifluoperazine.
Embodiment 8
[0119] The method of any one of Embodiments 1-5 wherein the
antipsychotic drug is an atypical antipsychotic drug.
Embodiment 9
[0120] The method of Embodiment 8 wherein the atypical
antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone.
Embodiment 10
[0121] The method of any one of Embodiments 1-9 wherein the
therapeutically effective amount of the antipsychotic drug is 10 to
90% less than the therapeutically effective amount of the
antipsychotic drug when administered in the absence of the VMAT2
inhibitor.
Embodiment 11
[0122] The method of any one of Embodiments 1-9 wherein the
therapeutically effective amount of the antipsychotic drug is at
least 25% less than the therapeutically effective amount of the
antipsychotic drug when administered in the absence of the VMAT2
inhibitor.
Embodiment 12
[0123] The method of any one of Embodiments 1-9 wherein the
therapeutically effective amount of the antipsychotic drug is at
least 50% less than the therapeutically effective amount of the
antipsychotic drug when administered in the absence of the VMAT2
inhibitor.
Embodiment 13
[0124] The method of any one of Embodiments 1-12 wherein the VMAT2
inhibitor is tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one).
Embodiment 14
[0125] The method of any one of Embodiments 1-12 wherein the VMAT2
inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof.
Embodiment 15
[0126] The method of any one of Embodiments 1-12, wherein the VMAT2
inhibitor is (S)-2-Amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester.
Embodiment 16
[0127] The method of any one of Embodiments 1-12, wherein the VMAT2
inhibitor is
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol, or a precursor thereof.
Embodiment 17
[0128] The method of any one of Embodiments 1-12, wherein the VMAT2
inhibitor is
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ).
Embodiment 18
[0129] A pharmaceutical preparation for use in treating a
neuropsychiatric disorder, said preparation comprising an
antipsychotic drug and a VMAT2 inhibitor, wherein the preparation
comprises an amount of the antipsychotic drug that is a
subtherapeutic amount if used in the absence of the VMAT2
inhibitor.
Embodiment 19
[0130] The pharmaceutical preparation of Embodiment 18 wherein the
neuropsychiatric disorder is schizophrenia, schizoaffective
disorder, bipolar disorder, major depressive disorder (MDD), or
autism.
Embodiment 20
[0131] The pharmaceutical preparation of Embodiment 18 or
Embodiment 19, wherein the antipsychotic drug is a typical
antipsychotic drug.
Embodiment 21
[0132] The pharmaceutical preparation of Embodiment 20 wherein the
typical antipsychotic drug is fluphenazine, haloperidol, loxapine,
molindone, perphenazine, pimozide, sulpiride, thioridazine, or
trifluoperazine.
Embodiment 22
[0133] The pharmaceutical preparation of Embodiment 18 or
Embodiment 19 wherein the antipsychotic drug is an atypical
antipsychotic drug.
Embodiment 23
[0134] The pharmaceutical preparation of Embodiment 22, wherein the
atypical antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone.
Embodiment 24
[0135] The pharmaceutical preparation of any one of Embodiments
18-23, wherein the antipsychotic drug is formulated in a first
pharmaceutical composition and the VMAT2 inhibitor is formulated in
a second pharmaceutical composition.
Embodiment 25
[0136] The pharmaceutical preparation of any one of Embodiments
18-24, wherein the therapeutically effective amount of the
antipsychotic drug is 10 to 90% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor.
Embodiment 26
[0137] The pharmaceutical preparation of any one of Embodiments
18-24, wherein the therapeutically effective amount of the
antipsychotic drug is at least 25% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor.
Embodiment 27
[0138] The pharmaceutical preparation of any one of Embodiments
18-24, wherein the therapeutically effective amount of the
antipsychotic drug is at least 50% less than the therapeutically
effective amount of the antipsychotic drug when administered in the
absence of the VMAT2 inhibitor.
Embodiment 28
[0139] The pharmaceutical preparation of any one of Embodiments
18-27 wherein the VMAT2 inhibitor is tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one).
Embodiment 29
[0140] The pharmaceutical preparation of any one of Embodiments
18-27 wherein the VMAT2 inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof.
Embodiment 30
[0141] The pharmaceutical preparation of any one of Embodiments
18-27, wherein the VMAT2 inhibitor is (S)-2-Amino-3-methyl-butyric
acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester.
Embodiment 31
[0142] The pharmaceutical preparation of any one of Embodiments
18-27, wherein the VMAT2 inhibitor is
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol, or a precursor thereof.
Embodiment 32
[0143] The pharmaceutical preparation of any one of Embodiments
18-27, wherein the VMAT2 inhibitor is
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ).
Embodiment 33
[0144] A method for enhancing efficacy of an antipsychotic drug
comprising administering to a subject a combination of (a) the
antipsychotic drug, and (b) a VMAT2 inhibitor.
Embodiment 34
[0145] The method of Embodiment 33, wherein enhancing efficacy of
the antipsychotic drug comprises decreasing the amount of the
antipsychotic that is therapeutically effective.
Embodiment 35
[0146] The method of Embodiment 33, wherein the amount of the
antipsychotic drug that is therapeutically effective is 10 to 90%
less than the amount of the antipsychotic drug that is
therapeutically effective when the antipsychotic drug is
administered in the absence of the VMAT2 inhibitor.
Embodiment 36
[0147] The method of Embodiment 33, wherein the amount of the
antipsychotic drug that is therapeutically effective is at least
25% less than the amount of the antipsychotic drug that is
therapeutically effective when the antipsychotic drug is
administered in the absence of the VMAT2 inhibitor.
Embodiment 37
[0148] The method of Embodiments 33, wherein the amount of the
antipsychotic drug that is therapeutically effective is at least
50% less than the amount of the antipsychotic drug that is
therapeutically effective when the antipsychotic drug is
administered in the absence of the VMAT2 inhibitor.
Embodiment 38
[0149] The method of any one of Embodiments 33-37, wherein the
antipsychotic drug is a typical antipsychotic drug.
Embodiment 39
[0150] The method of Embodiment 38, wherein the typical
antipsychotic drug is fluphenazine, haloperidol, loxapine,
molindone, perphenazine, pimozide, sulpiride, thioridazine, or
trifluoperazine.
Embodiment 40
[0151] The method of any one of Embodiments 33-37, wherein the
antipsychotic drug is an atypical antipsychotic drug.
Embodiment 41
[0152] The method of Embodiment 40, wherein the atypical
antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone.
Embodiment 42
[0153] The method of any one of Embodiments 33-41, wherein the
VMAT2 inhibitor is tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one).
Embodiment 43
[0154] The method of any one of Embodiments 33-41, wherein the
VMAT2 inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof.
Embodiment 44
[0155] The method of any one of Embodiments 33-41, wherein the
VMAT2 inhibitor is (S)-2-Amino-3-methyl-butyric acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester.
Embodiment 45
[0156] The method of any one of Embodiments 33-41, wherein the
VMAT2 inhibitor is
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol, or a precursor thereof.
Embodiment 46
[0157] The method of any one of Embodiments 33-41, wherein the
VMAT2 inhibitor is
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ).
Embodiment 47
[0158] A pharmaceutical preparation comprising an antipsychotic
drug and a VMAT2 inhibitor, wherein the preparation is effective
for treating a neuropsychiatric disorder, and wherein the amount of
the antipsychotic drug is subtherapeutic compared with the
therapeutic amount of the antipsychotic drug when used alone for
treating the neuropsychiatric disorder in the absence of the VMAT2
inhibitor.
Embodiment 48
[0159] A pharmaceutical preparation comprising synergistically
effective amounts of an antipsychotic drug and a VMAT2
inhibitor.
Embodiment 49
[0160] The pharmaceutical preparation of Embodiment 47 or
Embodiment 48 wherein the antipsychotic drug is a typical
antipsychotic drug.
Embodiment 50
[0161] The pharmaceutical composition of Embodiment 49 wherein the
typical antipsychotic drug is fluphenazine, haloperidol, loxapine,
molindone, perphenazine, pimozide, sulpiride, thioridazine, or
trifluoperazine.
Embodiment 51
[0162] The pharmaceutical preparation of Embodiment 47 or
Embodiment 48 wherein the antipsychotic drug is an atypical
antipsychotic drug.
Embodiment 52
[0163] The pharmaceutical composition of Embodiment 51 wherein the
atypical antipsychotic drug is aripiprazole, asenapine, clozapine,
iloperidone, olanzapine, paliperidone, quetiapine, risperidone, or
ziprasidone.
Embodiment 53
[0164] The pharmaceutical preparation of any one of Embodiments
47-52 wherein the VMAT2 inhibitor is tetrabenazine
(3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoqui-
nolin-2-one).
Embodiment 54
[0165] The pharmaceutical preparation of any one of Embodiments
47-52 wherein the VMAT2 inhibitor is
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-ol (R,R,R DHTBZ), or a precursor thereof.
Embodiment 55
[0166] The pharmaceutical preparation of any one of Embodiments
47-52, wherein the VMAT2 inhibitor is (S)-2-Amino-3-methyl-butyric
acid
(2R,3R,11bR)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[-
2,1-a]isoquinolin-2-yl ester.
Embodiment 56
[0167] The pharmaceutical preparation of any one of Embodiments
47-52, wherein the VMAT2 inhibitor is
[(2R,3S,11bR)-9,10-Dimethoxy-3-(2-methylpropyl)-1H,2H,3H,4H,6H,7H,11bH-py-
rido[2,1-a]isoquinolin-2-yl]methanol, or a precursor thereof.
Embodiment 57
[0168] The pharmaceutical preparation of any one of Embodiments
47-52, wherein the VMAT2 inhibitor is
3-isobutyl-9,10-d.sub.6-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a-
]isoquinolin-2-one (d.sub.6-TBZ).
Embodiment 58
[0169] The pharmaceutical preparation of any one of Embodiments
47-57 wherein the antipsychotic drug and the VMAT2 inhibitor are
formulated in the same pharmaceutical composition with at least one
pharmaceutically acceptable excipient.
Embodiment 59
[0170] The pharmaceutical preparation of any one of Embodiments
47-58 wherein the antipsychotic drug is formulated in a first
pharmaceutical composition with at least one pharmaceutically
acceptable excipient and the VMAT2 inhibitor is formulated in a
second pharmaceutical composition with at least one
pharmaceutically acceptable excipient.
Embodiment 60
[0171] The pharmaceutical composition of any one of Embodiments
47-59 wherein the neuropsychiatric disorder is schizophrenia,
schizoaffective disorder, bipolar disorder, major depressive
disorder (MDD), or autism.
[0172] All U.S. patents, U.S. patent application publications, U.S.
patent applications, foreign patents, foreign patent applications
and non-patent publications referred to in this specification
and/or listed in the Application Data Sheet are incorporated herein
by reference, in their entirety. U.S. Patent Application No.
61/937,223, filed Feb. 7, 2014, is incorporated herein in its
entirety. Aspects of the embodiments can be modified, if necessary
to employ concepts of the various patents, applications and
publications to provide yet further embodiments.
[0173] These and other changes can be made to the embodiments in
light of the above-detailed description. Although specific
embodiments have been described herein for purposes of
illustration, various modifications may be made without departing
from the spirit and scope of the invention. In general, in the
following claims, the terms used should not be construed to limit
the claims to the specific embodiments disclosed in the
specification and the claims, but should be construed to include
all possible embodiments along with the full scope of equivalents
to which such claims are entitled. Accordingly, the claims are not
limited by the disclosure.
* * * * *