U.S. patent application number 15/395329 was filed with the patent office on 2017-11-23 for synthesis of udp-glucose: n-acylsphingosine glucosyltransferase inhibitors.
The applicant listed for this patent is Genzyme Corporation. Invention is credited to Bradford H. Hirth, Craig Siegel.
Application Number | 20170334888 15/395329 |
Document ID | / |
Family ID | 23182463 |
Filed Date | 2017-11-23 |
United States Patent
Application |
20170334888 |
Kind Code |
A1 |
Hirth; Bradford H. ; et
al. |
November 23, 2017 |
SYNTHESIS OF UDP-GLUCOSE: N-ACYLSPHINGOSINE GLUCOSYLTRANSFERASE
INHIBITORS
Abstract
Disclosed is a novel enantiomeric synthesis ceramide-like
inhibitors of UDP-glucose: N-acylsphingosine glucosyltransferase.
Also disclosed are novel intermediates formed during the
synthesis.
Inventors: |
Hirth; Bradford H.;
(Littleton, MA) ; Siegel; Craig; (Woburn,
MA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Genzyme Corporation |
Cambridge |
MA |
US |
|
|
Family ID: |
23182463 |
Appl. No.: |
15/395329 |
Filed: |
December 30, 2016 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
14315453 |
Jun 26, 2014 |
9546161 |
|
|
15395329 |
|
|
|
|
13420108 |
Mar 14, 2012 |
8779163 |
|
|
14315453 |
|
|
|
|
12830962 |
Jul 6, 2010 |
8138353 |
|
|
13420108 |
|
|
|
|
11895632 |
Aug 24, 2007 |
7763738 |
|
|
12830962 |
|
|
|
|
10916824 |
Aug 12, 2004 |
7265228 |
|
|
11895632 |
|
|
|
|
10197227 |
Jul 16, 2002 |
6855830 |
|
|
10916824 |
|
|
|
|
60305814 |
Jul 16, 2001 |
|
|
|
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
C07D 498/04 20130101;
A61P 1/16 20180101; A61P 25/28 20180101; A61P 27/02 20180101; A61P
7/06 20180101; C07D 295/185 20130101; A61P 3/00 20180101; A61P
35/00 20180101; C07D 319/18 20130101; A61P 43/00 20180101; C07D
405/06 20130101; A61P 17/02 20180101; A61K 31/4025 20130101 |
International
Class: |
C07D 405/06 20060101
C07D405/06; C07D 319/18 20060101 C07D319/18; C07D 295/185 20060101
C07D295/185; C07D 498/04 20060101 C07D498/04; A61K 31/4025 20060101
A61K031/4025 |
Claims
1-23: (canceled)
24: A method of preparing a compound of formula (2) ##STR00032##
said method comprising reacting a compound of formula (1)
##STR00033## with pyrrolidine, followed by hydrolysis.
25: The method of claim 24, wherein the hydrolysis is carried out
using methanol as solvent.
26: The method of claim 24, wherein the compound of formula (1) is
prepared by reacting (5S)-5-phenylmorpholin-2-one with
1,4-benzodioxan-6-carboxaldehyde.
27: The method of claim 26, wherein (5S)-5-phenylmorpholin-2-one is
prepared by reacting a compound of formula (i) ##STR00034## with a
compound of formula (ii) ##STR00035##
28: The method of claim 24, further comprising reducing the
compound of formula (2) to form a compound of formula (3)
##STR00036##
29: The method of claim 28, wherein the reduction is carried out
using lithium aluminum hydride.
30: A method of preparing a compound of formula (3) ##STR00037##
said method comprising reducing a compound of formula (2)
##STR00038##
31: The method of claim 30, wherein the reduction is carried out
using lithium aluminum hydride.
32: The method of claim 30, wherein the compound of formula (2) is
prepared by reacting a compound of formula (1) ##STR00039## with
pyrrolidine, followed by hydrolysis.
33: The method of claim 32, wherein the hydrolysis is carried out
using methanol as solvent.
34: The method of claim 32, wherein the compound of formula (1) is
prepared by reacting (5S)-5-phenylmorpholin-2-one with
1,4-benzodioxan-6-carboxaldehyde.
35: The method of claim 34, wherein (5S)-5-phenylmorpholin-2-one is
prepared by reacting a compound of formula (i) ##STR00040## with a
compound of formula (ii) ##STR00041##
Description
RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application
Ser. No. 10/916,824, filed Aug. 12, 2004, which is a divisional of
U.S. patent application Ser. No. 10/197,227, filed Jul. 16, 2002,
which claims the benefit of U.S. Provisional Application No.
60/305,814, filed Jul. 16, 2001. The entire teachings of these
applications are incorporated herein by reference.
BACKGROUND OF THE INVENTION
[0002] Glycosphingolipids (GSLs) are a class of naturally occurring
compounds which have a multitude of biological functions, including
the ability to promote cell growth, cell differentiation, adhesion
between cells or between cells and matrix proteins, binding of
microorganisms and viruses to cells, and metastasis of tumor cells.
GSLs are derived from glucosylceramide (GlcCer), which is produced
from ceramide and UDP-glucose by the enzyme UDP-glucose:
N-acylsphingosine glucosyltransferase (GlcCer synthase). The
structure of ceramide is shown below:
##STR00001##
[0003] The accumulation of GSLs has been linked to a number of
diseases, including Tay-Sachs, Gaucher's, and Fabry's diseases
(see, for example, U.S. Pat. No. 6,051,598). GSLs have also been
linked to certain cancers. For example, it has been found that
certain GSLs occur only in tumors or at abnormally high
concentrations in tumors; exert marked stimulatory or inhibitory
actions on tumor growth when added to tumor cells in culture media;
and inhibit the body's normal immunodefense system when shed by
tumors into the surrounding extracellular fluid. The composition of
a tumor's GSLs changes as the tumors become increasingly malignant
and antibodies to certain GSLs inhibit the growth of tumors.
[0004] Compounds which inhibit GlcCer synthase can lower GSL
concentrations and have been reported to be useful for treating a
subject with one of the aforementioned diseases. A number of potent
inhibitors of GlcCer, referred to herein as "amino ceramide-like
compounds", are disclosed in U.S. Pat. Nos. 6,051,598, 5,952,370,
5,945,442, 5,916,911 and 6,030,995. The term "ceramide-like
compounds" refers to analogs of ceramide in which: 1) the primary
alcohol is replaced with a substituted amino group; and 2) the
alkenyl group is replaced with an aryl group, preferably phenyl or
substituted phenyl. The corresponding N-deacylated compounds are
referred to as "sphingosine-like compounds."
[0005] Unfortunately, known methods of preparing amino
ceramide-like compounds are poorly suited for manufacturing on an
industrial scale. Because of the two chiral centers, most known
syntheses generate four diastereoisomers, resulting in the need to
separate diastereomers by chromatography and to isolate the desired
enantiomer by crystallization after derivitization with optically
active reagents, e.g., dibenzoyltartaric acid isomers (see, for
example, Inokuchi and Radin, Journal of Lipid Research 28:565
(1987)). Neither of the processes are amenable to large scale
preparations. Enantioselective synthesis of amino ceramide-like
compounds using diastereoselective reductions have been reported
(Mitchell, et al., J. Org. Chem. 63:8837 (1998) and Nishida, et
al., SYNLETT 1998:389 (1998)), but require over ten steps, some of
which utilized expensive reagents such as diisobutylaluminum
hydride (DIABAL) and Garner Aldehyde (tert-butyl(R)-(+)-4 formyl-2,
2-dimethyl-3-oxazolidine carboxylate). Thus, there is a critical
need for enantioselective syntheses of amino ceramide-like
compounds which are more economical and efficient, and involve
fewer steps than known syntheses.
SUMMARY OF THE INVENTION
[0006] Provided herein is an efficient, highly enantioselective
synthesis of amino ceramide-like compounds. This synthesis of amino
ceramide-like compounds involves only five steps from known
compounds. For example, the ceramide-like compound designated as
"Compound 5" in FIG. 2 was produced in an enantiomeric excess of at
least 99.6% and an overall yield of 9% (see Examples 1 and 2).
Novel intermediates prepared during the course of the synthesis are
also disclosed.
[0007] The present invention is directed is a method of preparing a
ceramide-like compound represented by Structural Formula (I):
##STR00002##
[0008] R.sub.1 is a substituted or unsubstituted aromatic group;
preferably, R.sub.1 is a substituted or unsubstituted phenyl group,
more preferably phenyl substituted in the meta/para positions with
--OCH.sub.2O--, --OCH.sub.2CH.sub.2O-- or in the para position with
halo, lower alkyl thiol, --OH, --O(phenyl), --OCH.sub.2(phenyl),
lower alkyl, amino, lower alkyl amino, lower dialkyl amino, or
--O(lower alkyl);
[0009] R.sub.2 and R.sub.3 are independently --H, a substituted or
unsubstituted aliphatic group or, taken together with the nitrogen
atom to which they are bonded, are a substituted or unsubstituted
non-aromatic heterocyclic ring.
[0010] R.sub.7 is a substituted or unsubstituted aliphatic group,
preferably a C1-C30 straight chain unsubstituted aliphatic group or
a C1-C30 straight chained aliphatic group substituted with one or
more C1-C2 alkyl groups, more preferably an unsubstituted C1-C30
straight chain alkyl or alkenyl group and even more preferably an
unsubstituted C7-C10 or C10-C16 straight chain alkyl or alkenyl
group.
[0011] The method of preparing a ceramide-like compound represented
by Structural Formula (I) comprises a first step whereby an amine
compound HNR.sub.2R.sub.3 is reacted, with a cyclic starting
material represented by Structural Formula (II):
##STR00003##
[0012] The reaction between the amine compound HNR.sub.2R.sub.3 and
the cyclic starting material represented by Structural Formula (II)
forms an amide intermediate represented by Structural Formula
(III):
##STR00004##
In Structural Formulas (II) and (III), R.sub.1-R.sub.3 are as
described for Structural Formula (I); and R.sub.5 is a substituted
or unsubstituted aromatic group, preferably a substituted or
unsubstituted phenyl group.
[0013] The method of preparing a ceramide-like compound represented
by Structural Formula (I) comprises a second step whereby the amino
acetal group in the intermediate represented by Structural Formula
(III) is hydrolyzed to form the acyclic compound represented by
Structural Formula (IV).
##STR00005##
R.sub.1, R.sub.2, R.sub.3 and R.sub.5 in Structural Formulas (IV)
are as defined in Structural Formulas (I)-(III).
[0014] The method of preparing a ceramide-like compound represented
by Structural Formula (I) comprises a third step whereby the
acyclic precursor compound represented by Structural Formula (IV)
is reacted with an amide reducing agent to form a compound
represented by Structural Formula (V):
##STR00006##
R.sub.1, R.sub.2, R.sub.3 and R.sub.5 in Structural Formula (V) are
as defined in Structural Formulas (I)-(IV).
[0015] The method of preparing a ceramide-like compound represented
by Structural Formula (I) comprises a fourth step whereby the
--NHCH(--CH.sub.2OH)R.sub.5 group of the amine compound represented
by Structural Formula (V) is debenzylated to form a
sphingosine-like compound represented by Structural Formula
(VI):
##STR00007##
Preferably, the debenzylation is achieved by hydrogenation.
R.sub.1, R.sub.2 and R.sub.3 are as described for Structural
Formulas (I)-(V).
[0016] The method of preparing a ceramide-like compound represented
by Structural Formula (I) comprises a fifth step whereby the
sphingosine-like compound represented by Structural Formula (VI) is
acylated to form the ceramide-like compound represented by
Structural Formula (I).
[0017] Other embodiments of the present invention include each of
the individual reactions described above, taken separately and in
combination with the other reactions.
[0018] Other embodiments of the present invention are intermediates
in the preparation of the ceramide-like compound represented by
Structural Formula (I) by the methods disclosed herein. In one
example, the present invention is directed to an intermediate
represented by Structural Formula (VII):
##STR00008## [0019] R.sub.1-R.sub.3 and R.sub.5 are as described
above for Structural Formulas (I)-(VI); and R.sub.4 is H.sub.2 or
O.
[0020] In another embodiment, the present invention is directed to
an intermediate represented by Structural Formula (VIII):
##STR00009## [0021] R.sub.4 is H.sub.2 or O; and [0022] R.sub.6 is
represented by Structural Formula (IX):
##STR00010##
[0022] Phenyl ring A in Structural Formula (IX) is substituted or
unsubstituted. Preferably, however, phenyl ring A is unsubstituted.
Alternatively, R.sub.4 in Structural Formula (VIII) is H.sub.2 and
R.sub.6 is --H.
[0023] In another embodiment, the present invention is directed to
an intermediate represented by Structural Formula (X):
##STR00011##
[0024] R.sub.5 in Structural Formula (X) is as defined for
Structural Formula (I).
[0025] The methods of the present invention can be utilized to
prepare ceramide-like compounds that inhibit the enzyme GlcCer
synthase in five steps from known starting materials. The synthesis
is highly efficient, resulting in an overall yield that is
generally greater than 8% and in an enantiomeric excess that is
typically greater than 99%. The synthesis utilizes inexpensive
reagents and therefore provides an economical route to potent
inhibitors of GlcCer synthase.
BRIEF DESCRIPTION OF THE DRAWINGS
[0026] FIG. 1 is a schematic showing the synthesis of ceramide-like
compounds represented by Structural Formula (I) using the methods
and intermediates disclosed herein.
[0027] FIG. 2 is a schematic showing the synthesis of ceramide-like
Compound (5) using the methods disclosed herein.
[0028] FIG. 3 is a schematic showing the synthesis of ceramide-like
compound (13) using the methods disclosed herein.
[0029] FIG. 4 shows the structures of Compounds (5)-(8).
DETAILED DESCRIPTION OF THE INVENTION
[0030] Described herein is a five step synthesis of amino
ceramide-like compounds from known starting materials. The
synthesis begins with the preparation of the cyclic starting
material represented by Structural Formula (II). The cyclic
starting material is reacted with a suitable amine, thereby opening
the lactone ring and forming the amide intermediate represented by
Structural Formula (III). The amino acetal in the amide
intermediate is hydrolyzed to form the acyclic compound represented
by Structural Formula (IV). The amide of this acyclic compound is
reduced with an amide reducing agent to form an amine compound
represented by Structural Formula (V), which is in turn
debenzylated to form the sphingosine-like compound represented by
Structural Formula (VI). The primary amine of the sphingosine-like
compound represented by Structural Formula (VI) can then be
acylated to form an amino ceramide-like compound. This synthesis is
shown schematically in FIG. 1. A detailed description of each
reaction in the synthesis is provided below.
[0031] The cyclic starting material represented by Structural
Formula (II) is prepared according to methods described in Alker,
et al., Tetrahedron 54:6089 (1998) and Harwood and Robertson, Chem.
Commun. 1998:2641 (1998). Specifically,
(5S)-5-phenylmorpholin-2-one is reacted with at least two
equivalents and preferably from about 2.5 to about 5.0 equivalents
of aryl aldehyde R.sub.1CHO under dehydrating conditions. R.sub.1
is as defined in Structural Formula (I). "Dehydrating conditions"
refer to conditions under which water is removed from the reaction
mixture. Removal of water can be achieved, for example, by carrying
out the reaction in presence of a reagent (a "dehydrating reagent")
that reacts with water (e.g., molecular sieves) but is
substantially inert towards the other reagents present in the
reaction mixture, or removal of water can also be achieved by
azeotroping with a solvent such as toluene. Sufficient dehydrating
reagent is used to remove the two equivalents of water (relative to
cyclic starting material) released during the reaction. The
concentration of reagents if typically between about 0.01 M and
about 5.0 M, more typically between about 0.1 M and about 1.0 M;
suitable reaction temperatures range between about 50.degree. C.
and about 150.degree. C., preferably between about 100.degree. C.
and about 120.degree. C.
[0032] The cyclic starting material is converted to the amide
intermediate represented by Structural Formula (II) by reacting the
cyclic starting material with the amine NHR.sub.2R.sub.3 under
conditions suitable for amidating an ester with an amine. Such
conditions are well known in the art and are described, for
example, in March, "Advanced Organic Chemistry--Reactions,
Mechanisms and Structure", Third Edition, John Wiley & Sons,
1985, pages 375-76, and references cited therein. Although an
excess of either reagent can be used, cyclic starting material is
more commonly the limiting reagent. Generally up to about fifteen
equivalents of amine relative to cyclic starting material are used,
typically up to about eight equivalents. The reaction can be done
neat, however, it is more usually carried out in a aprotic,
non-nucleophilic, solvent at amine concentrations as dilute as 0.01
M. Amine concentrations are more typically, however, between about
0.4 M and about 4.0 M. Suitable solvents include halogenated
solvents such as chloroform, dichloromethane and
1,2-dichloroethane, acetonitrile, dimethylformamide (DMF), ethereal
solvents such as diethyl ether, tetrahydrofuran (THF) and
1,4-dioxane and aromatic solvents such as benzene and toluene.
Suitable reaction temperatures generally range from about 0.degree.
C. to about 100.degree. C., typically between about 25.degree. C.
to about 35.degree. C.
[0033] Conditions for hydrolyzing aminoacetals are known in the art
and are described, for example, in March, "Advanced Organic
Chemistry--Reactions, Mechanisms and Structure", Third Edition,
John Wiley & Sons, 1985, pages 329-32, and references cited
therein. For example, the aminoacetal group in the amide
intermediate represented by Structural Formula (III) can be
hydrolyzed with dilute aqueous mineral acid. Suitable acids include
hydrochloric acid, sulfuric acid or phosphoric acid, although
hydrochloric is the most common choice. Organic acids such as
acetic acid and sulfonic acids (e.g., methansulfonic acid,
toluenesulfonic acid, trifluoromethylsulfonic acid and the like)
can also be used. At least one equivalent of acid relative to the
intermediate is typically used, but an excess of acid is preferred
to ensure complete hydrolysis, for example, excesses of at least
ten fold, preferably an excess of about two to about three fold and
more preferably between about 10-50%. The concentration of acid in
the reaction mixture is generally between about 0.05 M to about 1.0
M, typically between about 0.1 M and about 0.5 M. An organic
co-solvent miscible with water is often used to solubilize the
intermediate. Examples include alcohols such as methanol or ethanol
and DMF. Common solvent ratios of organic solvent to water range
between about 1:1 to about 8:1. Suitable reaction temperatures
range from ambient temperature to about 100.degree. C., preferably
between about 60.degree. C. to about 80.degree. C. Alternatively,
the amino acetal can be hydrolyzed with Lewis acids such as
trimethylsilyl iodide, wet silica gel or LiBF.sub.4 in wet
acetonitrile, as described in March, supra.
[0034] An "amide reducing agent" Is a reagent which can reduce an
amide to an amine. Such reagents are known in the art and are
disclosed in, for example, in March, "Advanced Organic
Chemistry--Reactions, Mechanisms and Structure", Third Edition,
John Wiley & Sons, 1985, pages 1099-1100, Brown and
Krishnamurthy, Aldrichimica Acta 12:3 (1979) and references cited
therein. Examples include lithium aluminum hydride, lithium
triethyl borohydride, borane reagents (e.g.,
borane.cndot.tetrahydrofuran, borane.cndot.methyl sulfide,
disiamylborane, and the like), aluminum hydride, lithium trimethoxy
aluminum hydride and triethyloxonium fluoroborate/sodium
borohydride. In the method of the present invention, lithium
aluminum hydride is the most commonly used amide reducing agent.
Although as little as 0.5 equivalents of lithium aluminum hydride
relative to amide starting material can be used, it is more common
to use an excess, often up to about five equivalents. Preferably,
between about 1.5 and about 2.5 equivalents of lithium aluminum
hydride are used relative to amine starting material. Ethereal
solvents are typically used for the reduction; examples include
diethyl ether, THF, glyme, diglyme and 1,4-dioxane. Suitable
concentrations of reducing agent are generally between about 0.1 M
and about 5.0 M, more typically between about 0.8 M and about 1.5
M. The reduction Is most commonly carried out at ambient
temperature, but temperatures between about 0.degree. C. and about
80.degree. C. or 100.degree. C. can also be used.
[0035] To form the sphingosine-like compound represented by
Structural Formula (VI), the amine Compound represented by
Structural Formula (V) is debenzylated. The term "debenzylating" is
used herein to refer to cleaving the carbon-nitrogen bond of a
group --NH--CH.sub.2Z, wherein Z is an aryl group, preferably
phenyl. Optionally, the methylene group can be replaced with a
methine group. With respect to the sphingosine-like compound
represented by Structural Formula (VI), "debenzylation" refers to
converting the --NHCH(--CH.sub.2OH)R.sub.5 group to --NH.sub.2,
Debenzylation conditions are well known in the art and are
disclosed, for example, in Greene and Wuts, "Protective Groups in
Organic Synthesis", John Wiley & Sons (1991), pages 384-86 and
references cited therein.
[0036] Preferably, debenzylation is achieved by hydrogenation under
a hydrogen atmosphere and in the presence of a hydrogenation
catalyst. Suitable hydrogen pressures are generally between about
atmospheric pressure and about 1000 pounds per square inch. Other
sources of hydrogen (e.g., formic acid, ammonium formate,
cyclohexene and the like) can also be used. Suitable hydrogenation
catalysts include 20% palladium hydroxide on carbon (Perlman's
catalyst), palladium chloride, palladium, platinum oxide and
palladium on carbon. Typically, between about 10% and about 100%
weight/weigh (w/w) relative to amino compound is used. In most
instances, an organic acid such as formic acid, acetic acid or
trifluoroacetic acid or an inorganic acid such as hydrochloric acid
or sulfuric acid is present, for example, between about one to
about five equivalents relative to amine compound, preferably
between about 1.6 to about 2.4 equivalents. The reaction is most
commonly carried out in an alcoholic solvent such as methanol or
ethanol with water as co-solvent (e.g., between 0% and about 50%
volume/volume (v/v), preferably between about 5% and about 15%
v/v). Reaction temperatures between about 0.degree. C. and about
50.degree. C. are suitable, preferably between about 25.degree. C.
and about 40.degree. C.
[0037] Many debenzylation conditions other than hydrogenation are
known in the art and are included in the present invention.
Examples include sodium metal and NH.sub.3 (see, for example, du
Vigneaud and Behrens, J. Biol. Chem. 117:27 (1937)),
CCl.sub.3CH.sub.5OCOCl, CH.sub.3CN (see, for example, Rawal, at
al., J. Org. Chem., 52:19 (1987)), Me.sub.3SiCH.sub.2CH.sub.3OCOCl,
THF, -50.degree. C., then 25.degree. C. overnight (see, for
example, Campbell, et al., Tetrahedron Lett., 28:2331 (1987)),
.alpha.-chloroethyl chloroformate and sodium hydroxide (see, for
example Olofson, et al., J. Org. Chem. 49:2081 (1984) and DeShong
and Kell, Tetrahedron Lett., 27:3979 (1986)), vinyl chloroformate
(see, for example, Olofson et al., Tetrahedron Lett., 1977:1567
(1977) and Cooley and Evain, Synthesis, 1989:1 (1989)), RuO.sub.4,
NH.sub.5, H.sub.2O (see, for example, Gao and Jones, J. Am. Chem.
Soc., 109:1275 (1987)) and m-chloroperoxybenzoic acid followed by
FeCl.sub.2, -10.degree. C. (see, for example, Monkovic, et al.,
Synthesis, 1985:770 (1985).
[0038] The sphingosine-like compound represented by Structural
Formula (VI) is converted to a ceramide-like compound by acylating
the free amine. Acylations of amine groups are well known in the
art and can be carried out, for example, by reacting the amine with
an acylating agent R.sub.7C(O)--X. R.sub.7 is as described above
for Structural Formula (I) and X is a leaving group that is readily
displaced by a primary amine. Conditions for this reaction are
described in, for example, in March, "Advanced Organic
Chemistry--Reactions, Mechanisms and Structure", Third Edition,
John Wiley & Sons, 1985 and references cited therein. Examples
of suitable acylating agents include acid halides, anhydrides or
esters. Preferably, the amine is acylated with an acid chloride.
Generally, equimolar amounts of the sphingosine-like compound and
the acid chloride are used in the presence of a small excess,
relative to the acid chloride, of a tertiary amine such as
triethylamine, diisopropylethylamine, dimethylaminopyridine or
pyridine is used. However, an excess of acid chloride (typically
about 10-50%) can be used when the sphingosine-like compound is
limiting, and vice versa. The concentrations of the reagents in the
reaction mixture normally vary between about 0.005 M and about 5.0
M, and are preferably between about 0.05 M and about 0.5 M. The
excess of amine base can be greater than about 100%, but is
typically between about 5% and about 25%. Aprotic solvents such as
halogenated solvents are preferred (e.g., chloroform, methylene
chloride and 1,2-dichloromethane), however other aprotic solvents
such as ethereal solvents and hydrocarbon solvents can be suitable
substitutes. Ambient temperature is normally preferred for the
reaction, but temperatures between about 0.degree. C. and about
50.degree. can also be used.
[0039] Alternatively, the acylating agent is an activated ester
R.sub.7C(O)--OX', wherein --OX' is readily displaced by a primary
amine. Methods of acylating an amine with activated esters are
known in the art and are described in, for example, March,
"Advanced Organic Chemistry--Reactions, Mechanisms and Structure",
Third Edition, John Wiley & Sons, 1985, pages 371-375, and
references cited therein. Many activated esters are stable enough
to be isolated. N-Hydroxy succinimidyl esters, some of which are
commercially available from Aldrich Chemical Co., Milwaukee, Wis.,
are one example of activated esters of this type. Conditions
suitable for forming an amide with an acid chloride acylating
agent, described in the prior paragraph, can typically be used with
a stable activated ester. In contrast with acid chlorides, which
require activation with tertiary amines, activated esters are
reactive enough so that they form amides directly in the presence
of primary amines. Therefore, the tertiary amine can be omitted
from the acylation reaction when activated esters are used.
[0040] Alternatively, an activated ester is formed in situ.
Formation of an activated ester in situ requires a "coupling
agent", which is a reagent that replaces the hydroxyl group of a
carboxyl acid with a group which is susceptible to nucleophilic
displacement. Examples of coupling agents include
1,1'-carbonyldiimidazole (CDI), isobutyl chloroformate,
dimethylaminopropylethyl-carbodiimide (EDC), dicyclohexyl
carbodiimide (DCC). When amidating by in situ generation of an
activated ester, an excess of either the carboxylic acid or amine
can be used (typically a 50% excess, more typically about a 10-15%
excess). However, it is more common when carrying out the present
invention to use the amine compound as the limiting reagent.
Generally, from about 1.0 mole to about 10 moles of coupling agent
are used per mole of carboxylic acid, preferably from about 1.0
mole to about 1.5 moles of coupling agent per mole of carboxylic
acid. The reaction is generally carried out in aprotic solvents,
for example, halogenated solvents such as methylene chloride,
dichloroethane and chloroform, ethereal solvents tetrahydrofuran,
1,4-dioxane and diethyl ether and dimethylformamide. Suitable
reaction temperatures generally range from between about 0.degree.
to about 100.degree. C., but the reaction is preferably carried out
at ambient temperature.
[0041] Examples of specific conditions for carrying out the
reactions described herein are provided in Examples 1 and 2.
[0042] By utilizing the enantiomer of the compound represented by
Structural Formula (II) as the cyclic starting material, the
enantiomer of the compounds represented by Structural Formulas
(III)-(VI) and (I) can be prepared by utilizing the methods
described herein. The enantiomer of the cyclic starting material
represented by Structural Formula (III) can be prepared by reacting
(SR)-5-phenylmorpholin-2-one with two equivalents of the aldehyde
R.sub.1CHO under dehydrating conditions, as described above. The
enantiomer of compounds represented by Structural Formula (III),
(VII), (VIII) and (X) and methods of preparing the enantiomers of
the compounds represented by Structural Formulas (II)-(VI) and (I)
using procedures disclosed herein are encompassed within the
present invention.
[0043] The term "enantiomer" as it used herein, and structural
formulas depicting an enantiomer are meant to include the "pure"
enantiomer free from its optical isomer as well as mixtures of the
enantiomer and its optical isomer in which the enantiomer is
present in an enantiomeric excess, e.g., at least 10%, 25%, 50%,
75%, 90%, 95%, 98%, or 99% enantiomeric excess.
[0044] With regard to the variables R.sub.1-R.sub.5 in Structural
Formulas (I)-(IX), an "aliphatic group" is non-aromatic, consists
solely of carbon and hydrogen and may optionally contain one or
more units of unsaturation, e.g., double and/or triple bonds. An
aliphatic group may be straight chained, branched or cyclic. When
straight chained or branched, an aliphatic group typically contains
between about 1 and about 30 carbon atoms, more typically between
about 1 and about 24 carbon atoms. When cyclic, an aliphatic group
typically contains between about 3 and about 10 carbon atoms, more
typically between about 3 and about 7 carbon atoms. Aliphatic
groups are preferably lower alkyl groups, which include C1-30
straight chained or branched saturated hydrocarbons, preferably
C1-C24 straight chained or branched saturated hydrocarbons.
Examples include methyl, ethyl, n-propyl, Iso-propyl, n-butyl,
sec-butyl and tert-butyl.
[0045] Aromatic groups include carbocyclic aromatic groups such as
phenyl, 1-naphthyl, 2-naphthyl, 1-anthracyl and 2-anthacyl, and
heterocyclic aromatic groups such as N-imidazolyl, 2-imidazole,
2-thienyl, 3-thienyl, 2-furanyl, 3-furanyl, 2-pyridyl, 3-pyridyl,
4-pyridyl, 2-pyrimidy, 4-pyrimidyl, 2-pyranyl, 3-pyranyl,
3-pyrazolyl, 4-pyrazolyl, 5-pyrazolyl, 2-pyrazinyl, 2-thiazole,
4-thiazole, 5-thiazole, 2-oxazolyl, 4-oxazolyl and 5-oxazolyl.
[0046] Aromatic groups also include fused polycyclic aromatic ring
systems in which a carbocyclic aromatic ring or heteroaryl ring is
fused to one or more other heteroaryl rings. Examples include
2-benzothienyl, 3-benzothienyl, 2-benzofuranyl, 3-benzofuranyl,
2-indolyl, 3-indolyl, 2-quinolinyl, 3-quinolinyl, 2-benzothiazole,
2-benzooxazole, 2-benzimidazole, 2-quinolinyl, 3-quinolinyl,
1-isoquinolinyl, 3-quinolinyl, 1-isoindolyl and 3-isoindolyl.
[0047] Non-aromatic heterocyclic rings are non-aromatic carbocyclic
rings which include one or more heteroatoms such as nitrogen,
oxygen or sulfur in the ring. The ring can be five, six, seven or
eight-membered. Examples include morpholinyl, thiomorpholinyl,
pyrrolidinyl, piperazinyl, piperidinyl, azetidinyl, azacycloheptyl,
or N-phenylpiperazinyl.
[0048] Suitable substituents on a lower alkyl, aliphatic, aromatic,
non-aromatic, heterocyclic or benzyl group are those which do not
substantially interfere with the reactions described herein.
"Interfering with a reaction" refers to substantially decreasing
the yield (e.g., a decrease of greater than 50%) or causing a
substantial amount of by-product formation (e.g., where by-products
represent at least 50% of the theoretical yield). Interfering
substituents can be used, provided that they are first converted to
a protected form. Suitable protecting groups are known in the art
and are disclosed, for example, in Greene and Wuts, "Protective
Groups in Organic Synthesis", John Wiley & Sons (1991).
[0049] Suitable substituents on an alkyl, aliphatic, aromatic,
non-aromatic heterocyclic ring or benzyl group include, for
example, halogen (--Br, --Cl, --I and --F), --OR, --CN, --NO.sub.2,
--NR.sub.2, --COOR, --CONR.sub.2, --SO.sub.kR (k is 0, 1 or 2) and
--NH--C(.dbd.NH)--NH.sub.2. Each R is independently --H, an
aliphatic group, a substituted aliphatic group, a benzyl group, a
substituted benzyl group, an aromatic group or a substituted
aromatic group, and preferably --H, a lower alkyl group, a benzylic
group or a phenyl group. A substituted non-aromatic heterocyclic
ring, benzylic group or aromatic group can also have an aliphatic
or substituted aliphatic group as a substituent. A substituted
alkyl or aliphatic group can also have a non-aromatic heterocyclic
ring, benzyl, substituted benzyl, aromatic or substituted aromatic
group as a substituent. A substituted alkyl, substituted aliphatic,
substituted non-aromatic heterocyclic, substituted aromatic or
substituted benzyl group can have more than one substituent.
[0050] When R.sub.1 is a substituted phenyl group, examples of
preferred substitutents include --OCH.sub.2O--,
--OCH.sub.2CH.sub.2O--, halo, (lower alkyl)O--, lower alkyl thiol,
lower dialkylamine, --OH, --O(phenyl), --OCH.sub.2(phenyl), lower
alkyl, amine and lower alkyl amino.
[0051] When R.sub.5 is a substituted phenyl group, examples of
preferred substitutents include halo, (lower alkyl)O--, --O(phenyl)
and lower alkyl.
[0052] In the structural formulas depicted herein, the remainder of
the molecule or compound to which a chemical group or moiety is
connected is indicated by the following symbol: [0053] "" For
example, the corresponding symbol in Structural Formula (IX)
indicates that the depicted group, which is represented by R.sub.6
in Structural Formula (VIII), is connected via the benzylic carbon
to the amine in Structural Formula (VIII) by a single covalent
bond.
[0054] In preferred embodiments of the present invention the
variables used herein are defined as follows: R.sub.1 is a
substituted or unsubstituted phenyl group; R.sub.2 and R.sub.3 are
independently --H, an unsubstituted C1-C5 alkyl group or, taken
together with the nitrogen atom to which they are bonded, are an
unsubstituted C3-C10 non-aromatic heterocyclic ring; R.sub.5 is a
substituted or unsubstituted phenyl group, preferably phenyl; and
R.sub.7 is a C1-C30 straight chain unsubstituted aliphatic group or
a C1-C30 straight chained aliphatic group substituted with one or
more C1-C2 alkyl group and more preferably an unsubstituted C1-C30
straight chain alkyl or alkenyl group.
[0055] In another preferred embodiment, --NR.sub.2R.sub.3, taken
together, is pyrrolidinyl. More preferably, --NR.sub.2R.sub.3,
taken together, is pyrrolidinyl and R.sub.5 is phenyl in compounds
comprising R.sub.2, R.sub.3, and R.sub.5. Even more preferably, in
compounds comprising R.sub.1, R.sub.2, R.sub.3, and R.sub.5,
R.sub.1 is a substituted or unsubstituted phenyl group (preferably
phenyl substituted in the meta/para positions with --OCH.sub.2O--,
--OCH.sub.2CH.sub.2O-- or in the para position with halo, lower
alkyl thiol, --OH, --O(phenyl), --O--CH.sub.2(phenyl), lower alkyl,
amino, lower alkyl amine, lower dialkyl amino, or --O(lower alkyl),
--NR.sub.2R.sub.3, taken together, is pyrrolindinyl and R.sub.5 is
phenyl.
[0056] In another preferred embodiment, --NR.sub.2R.sub.3, taken
together, is piperidyl. More preferably, --NR.sub.2R.sub.3, taken
together, is piperidyl and R.sub.5 is phenyl in compounds
comprising R.sub.2, R.sub.3 and R.sub.5. Even more preferably, in
compounds comprising R.sub.1, R.sub.2, R.sub.3 and R.sub.5, R.sub.1
is a substituted or unsubstituted phenyl group (preferably phenyl
substituted in the meta/pare positions with --OCH.sub.2O--,
--OCH.sub.2CH.sub.2O-- or in the para position with halo, lower
alkyl thiol, --OH, --O(phenyl), --OCH.sub.2-(phenyl), --OCH.sub.2
(phenyl), lower alkyl, amino, lower alkyl amino, lower dialkyl
amino, or --O(lower alkyl), --NR.sub.2R.sub.3, taken together, is
piperidyl and R.sub.5 is phenyl.
[0057] Examples of ceramide-like compounds which can be prepared by
the methods of the present invention are represented by Structural
Formula (XI):
##STR00012##
[0058] R.sub.1 is phenyl substituted in the metal/para positions
with --OCH.sub.2O-- or --OCH.sub.2CH.sub.2O-- or in the par
position with halo, CH.sub.3O--, CH.sub.3CH.sub.2O--,
CH.sub.3CH.sub.2CH.sub.2O--, CH.sub.3(CH.sub.3)CHO--, CH.sub.3--,
CH.sub.3CH.sub.2--, CH.sub.3CH.sub.3CH.sub.2--,
CH.sub.3(CH.sub.3)CH--, --OH or --OCH.sub.2(phenyl); and R.sub.7 is
CH.sub.3(CH.sub.2).sub.n-- or
CH.sub.3(CH.sub.2).sub.n-2CH.dbd.CH--, wherein n is an integer from
0 to about 30. Preferably, n is 7, 8, 9, 10, 11, 12, 13, 14, 15,
16, 17, 18, 19, 20, 21, 22, 23 or 24. More preferably, R.sub.1 is
phenyl substituted meta/para with --OCH.sub.2CH.sub.2O--.
[0059] Compounds of this invention which possess a sufficiently
acidic, a sufficiently basic, or both functional groups, and
accordingly can react with any of a number of inorganic bases, and
inorganic and organic acids, to form a salt. Thus, the present
invention also includes salts of the intermediates represented by
Structural Formulas (VII), (VIII) and (X). Physiologically
acceptable salts are preferred. Acids commonly employed to form
acid addition salts are inorganic acids such as hydrochloric acid,
hydrobromic acid, hydroiodic acid, sulfuric acid, phosphoric acid,
and the like, and organic acids such asp-toluenesulfonic acid,
methanesulfonic acid, oxalic acid, p-bromophenyl-sulfonic acid,
carbonic acid, succinic acid, citric acid, benzoic acid, acetic
acid, and the like. Examples of such salts include the sulfate,
pyrosulfate, bisulfate, sulfite, bisulfite, phosphate,
monohydrogenphosphate, dihydrogenphosphate, metaphosphate,
pyrophosphate, chloride, bromide, iodide, acetate, propionate,
decanoate, caprylate, acrylate, formate, isobutyrate, caproate,
heptanoate, propiolate, oxalate, malonate, succinate, suberate,
sebacate, fumarate, maleate, butyne-1,4-dioate, hexyne-1,6-dioate,
benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate,
hydroxybenzoate, methoxybenzoate, phthalate, sulfonate,
xylenesulfonate, phenylacetate, phenylpropionate, phenylbutyrate,
citrate, lactate, gamma-hydroxybutyrate, glycolate, tartrate,
methanesulfonate, propanesulfonate, naphthalene-1-sulfonate,
naphthalene-2-sulfonate, mandelate, and the like.
[0060] Base addition salts include those derived from inorganic
bases, such as ammonium or alkali or alkaline earth metal
hydroxides, carbonates, bicarbonates, and the like. Such bases
useful in preparing the salts of this invention thus include sodium
hydroxide, potassium hydroxide, ammonium hydroxide, potassium
carbonate, and the like.
[0061] The entire teachings of the publications cited in this
application are incorporated herein by reference.
EXEMPLIFICATION
Example 1--Small Scale Preparation of Ceramid-Like Compounds
Intermediate 1
(1R,3S,5S,8aS)-1,3-Bis-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-5-phenyl-tet-
rahydro-oxazolo[4,3-c][1,4]oxazin-8-one
##STR00013##
[0063] To a stirred solution of (5S)-5-phenylmorpholin-2-one (2.00
g, 11.3 mmol) (prepared as in: Dellaria, J. F.: Santarsiero, B. D.
J. Org. Chem., 1989, 54, 3916) and 1,4-benzodioxan-6-carboxaldehyde
(5.56 g, 33.9 mmol) in toluene (125 mL) was added 4 .ANG. molecular
sieves (approximately 20 mL). The mixture was heated at reflux for
72 hours, filtered free of sieves and concentrated. The resulting
amber gum was flash chromatographed over silica (diethyl
other/hexane) to furnish a pale yellow solid. This material was
further purified by trituration with diethyl ether to afford 1.89 g
(34%) product as a fluffy white solid: .sup.1H NMR (CDCl.sub.3)
.delta. 7.31-7.17 (m, 5H), 6.95-6.79 (m, 5H), 5.32-5.27 (m, 2H),
4.43-4.28 (m, 2H), 4.24 (s, 4H), 4.18 (m, 4H), 4.16-4.08 (m, 2H)
ppm.
Intermediate 2
(2S,3R,1''S)-3-(2',3'-Dihydro-benzol[1,4]dioxin-6'-yl)-3-hydroxy-2-(2''-hy-
droxy-1''-phenyl-ethylamino)-1-pyrrolidin-1-yl-propen-1-one
##STR00014##
[0065] To a stirred solution of Intermediate 1 (1.80 g, 3.69 mmol)
in chloroform (20 mL) was added pyrolidine (2.0 mL, 24 mmol). The
solution was stirred overnight and then concentrated. The resulting
colorless tacky foam was taken up in methanol (16 mL) and 1 N
hydrochloric acid (4 mL). The mixture was refluxed for 1 hour,
treated with additional 1 N hydrochloric acid (2 mL) and refluxed
for another 2 hours. The reaction solution was concentrated and the
resulting residue partitioned between ethyl acetate and aqueous
sodium bicarbonate solution. The organic layer was dried (sodium
sulfate) and concentrated. The resulting pale yellow gum was
purified by flash chromatography over silica gel (methylene
chloride/2 N methanolic ammonia) to afford 1.40 g (92%) of
Intermediate 2 as a colorless foamy solid: .sup.1H NMR (CDCl.sub.3)
.delta. 7.31-7.13 (m, 5H), 6.93-6.70 (m, 3H), 4.47 (d, J=8.5, 1H),
4.18 (a, 4H), 3.82 (t, J=5.9, 1 H), 3.74 (d, J=6.0, 2H), 3.06 (d,
J=8.5, 1), 3.06-2.97 (m, 1H), 2.92-2.83 (m, 1H), 1.97-1.87 (m, 1H),
1.45-1.15 (m, 4H) ppm.
Intermediate 3
(1R,2R,1''S)-1-(2',3'-Dihydro-benzo[1,4]dioxin-6'-yl)-2-(2''-hydroxy-1''-p-
henyl-ethylamino)-3-pyrrolidin-1-yl-propan-1-ol
##STR00015##
[0067] To a stirred solution of Intermediate 2 (1.38 g, 3.35 mmol)
in tetrahydrofuran (30 mL) was added lithium aluminum hydride (0.26
g, 6.9 mmol). The foamy suspension was stirred overnight and then
quenched with the addition (dropwise until frothing ceases) of 1 N
aqueous sodium hydroxide (13 mL). The mixture was diluted with
water and extracted with ethyl acetate. The organic layer was dried
(sodium sulfate) and concentrated to afford a colorless gum. Flash
chromatography over silica gel (methylene chloride/2 N methanolic
ammonia) afforded 0.94 g (70%) of product as a colorless tacky
foam: .sup.1H NMR (CDCl.sub.3) .delta. 7.36-7.17 (m, 5H), 6.88-6.74
(m, 3H), 4.42 (d, J=5.4, 1H), 4.26 (s, 4H), 3.79-3.69 (m, 1H),
3.64-3.56 (m, 1H), 3.55-3.45 (m, 1H), 3.00-2.90 (m, 1H), 2.67-2.57
(m, 1H), 2.43-2.32 (m, 4H), 2.25-2.15 (m, 1H), 1.75-1.65 (m, 4H)
ppm.
Intermediate 4
(1R,2R)-2-Amino-1-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-3-pyrrolidin-1-yl-
-propan-1-ol
##STR00016##
[0069] In a high pressure reaction bomb equipped with a mechanical
stirrer was loaded a solution of intermediate 3 (0.91 g, 2.28 mmol)
in 10:1 methanol/water (22 mL), trifluoroacetic acid (0.18 mL, 2.3
mmol) and 20% palladium hydroxide on carbon (Perlman's catalyst;
0.91 g). The reactor was evacuated and backfilled with argon three
times and then evacuated and refilled with hydrogen (100 psi). The
reaction was stirred for 2 days and then evacuated and flushed with
nitrogen. The reaction solution was filtered through Celite and
concentrated. The resulting gray-green gum was flash
chromatographed over silica gel (methylene chloride/2 N methanolic
ammonia) to afford 0.165 g (26%) of product as a near colorless
gum: .sup.1H NMR (CDCl.sub.2) .delta. 6.89-6.76 (m, 3H), 4.54 (d,
J=3.7, 1H), 4.25 (s, 4H), 3.43 (s, 1H), 3.14-3.07 (m, 1H),
2.68-2.41 (m, 6H), 1.82-1.71 (m, 4H) ppm.
Compound 5
(1R,2R)-Hexadecanoic acid
[2-(2',3'-dihydro-benzol[1,4]dioxin-6'-yl)-2-hydroxy-1-pyrrolidin-1-ylmet-
hyl-ethyl]-amide
##STR00017##
[0071] To a stirred solution of Intermediate 4 (0.165 g, 0.593
mmol) in methylene chloride (8 mL) was added palmitoyl chloride
(0.18 g, 0.59 mmol) followed by N,N-diisopropylethylamine (0.11 mL,
0.65 mmol). The solution was stirred for 2 hours and then
concentrated. The residue was partitioned between ethyl acetate and
aqueous sodium bicarbonate solution. The organic layer was dried
(sodium sulfate) and concentrated. The resulting off-white solid
was flash chromatographed over silica gel (methylene chloride/2 N
methanolic ammonia) to afford 0.174 g (57%) of product as a white
solid. Comparisons by .sup.1H NMR spectroscopy and analytical
chiral HPLC (column: Chirex (S)-VAL and (R)-NE, 4.6.times.250 mm;
eluant: 0.5% trifluoroacetic acid in 67:31:2 hexane/methylene
chloride/ethanol; flow: 1 mL/min; detection 280 nM) demonstrate
that this material was identical to a sample of the same compound
prepared by the method of Polt, et al. (J. Org. Chem., 1998, 63,
8837). Enantiomeric excess was determined to be 99.6%. Total
contamination from the two possible diastereomers is determined to
be 0.2%. .sup.1H NMR (CDCl.sub.3) .delta. 6.88-6.73 (m, 3H), 5.84
(d, J=7.3, 1H), 4.90 (d, J=3.8, 1H), 4.24 (s, 4H), 4.22-4.15 (m,
1H), 2.86-2.72 (m, 2H), 2.72-2.55 (m, 4H), 2.10 (t, J=7.5, 2H),
1.82-1.74 (m, 4H), 1.58-1.46 (m, 2H), 1.32-1.16 (m, 24H), 0.88 (t,
J=6.7, 3H) ppm.
Example 2--Large Scale Preparation of Ceramide-Like Compounds
(5S)-5-Phenylmorpholin-2-One
##STR00018##
[0073] A solution of S-(+)-Phenyl glycinol (Aldrich, 10.17 g, 78.12
mmol) and Diisoptopylethylamine (Aldrich, 34 mL, 195 mmol, 2.5
equivalents) was prepared in CH.sub.3CN (200 mL). This solution was
added to phenyl-.alpha.-bromoacetate (18.48 g, 85.9 mnol, 1.1
equivalents) dissolved in CH.sub.3CN (50 mL) under nitrogen
dropwise over 2 hours. The resulting solution was stirred under
nitrogen for 16-20 hours. The solvent was removed by
rotoevaporation keeping the bath temperature at below 25.degree. C.
To the oil was added ethyl acetate (120 mL) and the mixture was
stirred for 15 minutes. The resulting white precipitate was
filtered off and the solid washed with ethyl acetate (25 mL). The
filtrate was rotoevaporated to an oil keeping the bath temperature
below 25.degree. C. After drying under vacuum for 0.5 hours, the
oil was dissolved in CH.sub.2Cl, (17 mL) and loaded onto a silica
gel column (60 g pecked with 10% ethyl acetate/hexanes. The upper
byproduct spots were eluted with 10% ethyl acetate/hexanes and the
product was eluted with 50% ethyl acetate/hexanes--100% ethyl
acetate. The fractions containing the product were rotoevaporated
to an oil keeping the bath temperature below 25.degree. C. This oil
was dissolved in ethyl acetate (12 mL) and hexanes (60 mL) was
added slowly in an ice bath to precipitate the product. The
resulting precipitate was filtered. The white to yellow solid was
vacuum dried. The (5S)-5-phenylmorpholin-2-one obtained (7.4 g,
41.8 mmol, 53%) was used directly in the next step.
Intermediate 1
(1R,3S,5S,8aS)-1,3-Bis-(2',3'-dihydro-benzol[1,4]dioxin-6'-yl)-5-phenyl-te-
trahydro-oxazolo[4,3-c][1,4]oxazin-8-one
##STR00019##
[0075] (5S)-5-Phenylmorpholin-2-one (7.4 g, 41.8 mmol) and
benzodioxolane-6-carboxaldehyde (Aldrich or Alfa Aesar, 20.56 g,
125.2 mmol, 3.0 equivalents) was dissolved in toluene (180 mL). The
solution was placed in a soxhlet extractor apparatus filled with 4
.ANG. molecular sieves (ca 30 g). The solution was refluxed under
nitrogen for 2-3 days. After cooling to room temperature, the
solvent was removed by rotoevaporation and the oil was dissolved in
ethyl acetate (200 mL). A solution of sodium bisulfite (Aldrich, 50
g) in water (100 mL) was added and the two phase mixture was
stirred at room temperature for 1 hour. The resulting white solid
was filtered off and washed with ethyl acetate. The filtrate was
placed in a separatory funnel and the layers separated. The organic
layer was washed with water (100 mL) and saturated sodium chloride
solution (100 mL). The dried (Na.sub.2SO.sub.4) solution was
filtered and rotoevaporated to a yellow-red foamy oil (23.11 g).
After drying under vacuum for 1 hour, diethyl ether (350 ml) was
added and the mixture was stirred at room temperature for 16-20
hours. The resulting white-yellow solid was filtered. The solid was
dried under vacuum. The cycloadduct was obtained in 46% yield (9.34
g).
Intermediate 2
(2S,3R,1''S)-3-(2',3'-Dihydro-benzo[1,4]dioxin-6'-yl)-3-hydroxy-2-(2''-hyd-
roxy-1''-phenyl-ethylamino)-1-pyrrolidin-1-yl-propan-1-one
##STR00020##
[0077] To the cycloadduct (Intermediate 1, 6.7 g, 13.74 mmol)
dissolved in methylene chloride (40 mL) was added pyrrolidine
(Aldrich, 5.7 mL, 68.7 mmol, 5 equivalents). The solution was
stirred under nitrogen at room temperature for 16-18 hours. The
solvent was rotoevaporated to yield a yellow foamy oil which was
vacuum dried for 0.5 hours. The crude was dissolved in methanol
(115 mL) and a 1 M aqueous HCl solution (115 mL) was added. The
solution was refluxed for 4 hours. After cooling to room
temperature, the methanol was removed by rotoevaporation. Ethyl
acetate (60 mL) was added and the two phase system was stirred at
room temperature for 5-15 minutes. The two layers were separated
and the organic layer was extracted with 1 M HCL (30 mL). The
combined aqueous layers were washed two times with ethyl acetate
(60, 30 mL). A saturated sodium bicarbonate solution (150 mL) was
added to the aqueous layer slowly. The product was extracted three
times with ethyl acetate (60 mL) from the basic (pH=8-9) aqueous
layer. The combined organic layers containing the product were
washed with a saturated sodium chloride solution (30 mL). After
drying with NaSO.sub.2SO.sub.4 the solution was filtered and
rotoevaporated to yield a yellow solid. Intermediate 2 was obtained
in 93% yield (5.26 g).
Intermediate 3
(1R,2R,1''S)-1-(2',3'-Dihydro-benzo[1,4]dioxin-6'-yl)-2-(2''-hydroxy-1''-p-
henyl-ethylamino)-3-pyrrolidin-1-yl-propan-1-ol
##STR00021##
[0079] To a 3-neck flask equipped with a dropping funnel and
condenser was added LiAIH.sub.4 (Aldrich, 1.2 g, 31.7 mmol, 2.5
equivalents) and anhydrous THF (20 mL) under nitrogen. A solution
of Intermediate 2 (5.23 g, 12.68 mmol) in anhydrous THF (75 mL) was
added dropwise to the reaction over 15-30 minutes. The reaction was
refluxed under nitrogen for 9 hours. The reaction was cooled in an
ice bath and a 1M NaOH solution was carefully added dropwise. After
stirring at room temperature for 15 minutes, water (50 mL) and
ethyl acetate (75 mL) was added. The layers were separated and the
aqueous layer was extracted twice with ethyl acetate (75 mL). The
combined organic layers were washed with saturated sodium chloride
solution (25 mL). After drying with Na.sub.2SO.sub.4 the solution
was filtered and rotoevaporated to yield a colorless to yellow
foamy oil. Intermediate 3 was obtained in 99% yield (5.3 g).
Intermediate 4
(1R,2R)-2-Amino-1-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-3-pyrrolidin-1-yl-
-propan-1-ol
##STR00022##
[0081] Intermediate 3 (5.3 g, 13.3 mmol) was dissolved in methanol
(60 mL). Water (6 mL) and trifluoroacetic acid (2.05 mL, 26.6 mmol,
2 equivalents) were added. After being placed under nitrogen, 20%
Palladium hydroxide on carbon (Pearlman's catalysis, Lancaster or
Aldrich, 5.3 g) was added. The mixture was placed in a Parr
Pressure Reactor Apparatus with glass insert. The apparatus was
placed under nitrogen and then under hydrogen pressure 110-120 psi.
The mixture was stirred for 2-3 days at room temperature under
hydrogen pressure 100-120 psi. The reaction was placed under
nitrogen and filtered through a pad of celite. The celite pad was
washed with methanol (100 mL) and water (100 mL). The methanol was
removed by rotoevaporation. The aqueous layer was washed with ethyl
acetate three times (100, 50, 50 mL). A 10 M NaOH solution (10 mL)
was added to the aqueous layer (pH=12-14). The product was
extracted from the aqueous layer three times with methylene
chloride (100, 100, 50 mL). The combined organic layers were dried
with Na.sub.2SO.sub.4, filtered and rotoevaporated to a colorless
oil. The foamy oil was vacuum dried for 2 h. Intermediate 4 was
obtained in 90% yield (3.34 g).
Compound 5
(1R,2R)-Hexadecanoic acid
[2-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-2-hydroxy-1-pyrrolidin-1-ylmeth-
yl-ethyl]amide
##STR00023##
[0083] To a solution of Intermediate 4 (3.34 &g, 12.0 mmol) in
methylene chloride (50 mL) was added a solution of palmitic acid
N-hydroxysuccinimide ester (Sigma, 4.24 g, 12.0 mmol) over 15-30
minutes under nitrogen at room temperature. The solution was
stirred at room temperature for 18-20 hours. To the reaction was
added methylene chloride (50 mL) and a 1 M NaOH solution (25 mL).
The two phase system was stirred at room temperature for 15-30 min.
Water (25 mL) was added and the layers were separated. The aqueous
layer was back extracted with methylene chloride (25 mL). The
combined organic layers were washed twice with water (25 mL) and
once with a saturated sodium chloride solution (25 mL). The organic
layer was dried with N.sub.2SO.sub.4, filtered and rotoevaporated
to a light yellow oil. The crude was recrystallized from hexane (50
mL). The white solid (5.46 g) obtained was separated on silica gel
(300 g) with 2% methanol:methylene chloride--4% methanol:methylene
chloride--4% 2 M ammonium in methanol:methylene chloride. The white
solid obtained was recrystallized form hexanes (70 mL). Compound 5
was obtained in 66% yield (4.18 g). Analytical chiral HPLC (column:
Chirex (S)-VAL and (R)-NE, 4.6.times.250 mm; eluant: 0.5%
trifluoroacetic acid in 67:31:2 hexane/methylene chloride/ethanol;
flow: 1 mL/min; detection: 280 nM) showed this material to be
98.98% pure with 0.89% of a diastereoisomer and 0.14% of the
enantiomer.
Example 3--Alternative Large Scale Preparation of Ceramide-Like
Compounds
(5S)-5-Phenylmorpholin-2-one HCl Salt
[0084] A solution of phenyl bromoacetate (Aldrich, 862.17 g, 4.0
moles, 1.1 equivalents) in acetonitrile (reagent grade, 1500 ml)
was cooled in an ice bath (internal temperature below 5.degree.
C.). To this was added a cold slurry (internal temperature below
5.degree. C.) of S-(+)-2-phenyl glycinol (Aldrich, 500 g, 3.65
moles, 1 equivalent) and diisopropylethylamine (DIPEA) (Aldrich,
1587 ml, 9.11 moles, 2.5 equivalents) in acetonitrile (2900 ml) in
portions while keeping the internal temperature below 10.degree. C.
The mixture was stirred at this temperature for 30 minutes before
the ice bath was removed and the mixture was allowed to stir at
room temperature for an additional 4 hours. The solvent was removed
in vacuo while maintaining the bath temperature at 25.degree. C.
The mixture was coevaporated with ethyl acetate (2.times.500 ml) to
produce a light yellow viscous oil. To the reaction mixture, ethyl
acetate (4500 ml) was added and the flask was immersed in an ice
bath with agitation. The mixture was allowed to cool below
8.degree. C. The solid was filtered and washed with ethyl acetate
(3.times.250 ml). The solution was cooled to below 5.degree. C. Dry
HCl gas was passed slowly into the solution while maintaining the
internal temperature below 15.degree. C. until the pH was below 2
(wet pH paper). The mixture was allowed to stir at this temperature
and pH for an additional 20 minutes before the solid was suction
filtered. The solid was washed with ethyl acetate (3.times.200 ml)
and dried under high vacuum for about 20 hours. The yield was 412 g
(53%). .sup.1H NMR was consistent with the
(5S)-5-phenylmorpholin-2-one HCl salt.
Intermediate 1
(1R,3S,6S,8aS)-1,3-Bis-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-S-phenyl-tet-
rahydro-oxazolo[4,3-c][1,4]oxazin-8-one
[0085] To a stirred suspension of (5S)-5-phenylmorpholin-2-one HCl
salt (381 g, 1 equivalent) in 15% ethyl acetate in toluene (2270
ml) was added a solution of sodium bicarbonate (1.1 equivalents) in
water (2000 ml). The resulting biphasic solution was stirred at
room temperature for about 1 hour. The organic layer was
transferred to a flask containing 1,4-benzodioxan-6-carboxaldehyde.
The flask was then equipped with a Dean-Stark unit, a condenser and
a nitrogen inlet. The mixture was heated at reflux with agitation
while about 650 ml solvent (mixture of ethyl acetate and toluene)
was collected via Dean-Stark unit. The resulting yellow-red
solution was allowed reflux for about 64 hours, under nitrogen
while the water formed during the reaction was collected in the
Dean-Stark unit. Most of the solvent was then removed via
distillation at atmospheric pressure through Dean-Stark unit. The
residual solvent was then removed by coevaporation with heptane
(500 ml) and tert-butylmethyl ether (2.times.725 ml) to produce a
yellow semi solid product. The semi solid product was dissolved in
ethyl acetate (3400 ml). A solution of sodium bisulfite (920 g) in
water (1500 ml) was added and the mixture was allowed to stir at
room temperature for about 1 hour. The solid that was formed was
removed by filtration and washed with ethyl acetate (3.times.400
ml). The filtrate was washed with water (1450 ml), 5% brine
solution (1450 ml) and dried over MgSO.sub.4 (100 g). The solvent
was removed in vacuo to afford a yellow solid. To this was added
tert-butylmethyl ether (2900 ml) and the suspension was stirred at
room temperature for 20 to 22 hours. The yellow solid was suction
filtered, washed with tort-butylmethyl ether (2.times.600 ml) and
dried under high vacuum at room temperature for about 22 hours. The
yield was 400.5 g (58%). .sup.1H NMR and TLC were consistent with
Intermediate 1.
Intermediate 2
2S,3R,1''S)-3-(2',3'-Dihydro-benzo[1,4]dioxin-6'-yl)-3-hydroxy-2-(2''-hydr-
oxy-1''-phenyl-ethylamino)-1-pyrrolidin-1-yl-propan-1-one
[0086] A solution of Intermediate 1 (312 g, 0.64 moles),
pyrrolidine (267 ml, 3.2 moles, 5 equivalents) and tetrahydrofuran
(1350 ml) was heated at reflux for 4.5 hours under nitrogen
atmosphere. The solvent and excess pyrrolidine were removed in
vacuo to produce the crude intermediate as an orange viscous oil.
The oil was dissolved in methanol (3000 ml) and 1M hydrochloric
acid solution (3000 ml). The resulting solution was heated at
reflux for about 7 hours. The solvent was then removed in vacuo to
afford a mixture of an oil and water. To this ethyl acetate (2000
ml) was added and the aqueous layer was separated. The organic
layer was extracted with aqueous 1M HCl (1000 ml). The aqueous
layers were combined and washed with ethyl acetate (2000 ml). The
aqueous layer was cooled in an ice bath. The pH of the aqueous
layer was adjusted to about 9 (pH paper) with 10 M aqueous NaOH
(525 ml). The aqueous layer was extracted with ethyl acetate (3000
ml). The organic layer was washed with 5% brine solution (1000 ml)
and dried (Na.sub.2SO.sub.4). The solvent was removed in vacuo to
produce a yellow viscous oil. The yield was 213.4 g, 81%. .sup.1H
NMR was consistent with Intermediate 2.
Intermediate 3
1R,2R,1''S)-1-(2',3'-Dihydro-benzo[1,4]dioxin-6'-yl)-2-(2''-hydroxy-1-phen-
yl-ethylamino)-3-pyrrolidin-1-yl-propan-1-ol
[0087] To a slurry of LiAlH.sub.4 (50.7 g, 1.34 moles, 2.6
equivalents) in tetrahydrofuran (700 ml) was added a solution of
Intermediate 2 (213.34 g, 0.517 moles) in tetrahydrofuran (2000 ml)
slowly with agitation at room temperature. The mixture was refluxed
for about 4 hours. TLC analysis (10% methanol in methylene
chloride, v/v) indicated consumption of the starting material. The
reaction mixture was cooled in an ice bath (below 5.degree. C.) and
water (135 ml) was added very slowly while keeping the internal
temperature less than or equal to 10.degree. C. To this was then
added a 15% aqueous NaOH solution (70 ml) followed by water (200
ml). The reaction mixture was allowed to warm to room temperature
while the agitation was continued. Methylene chloride (1000 ml) was
then added to the mixture and the salts were filtered through a pad
of celite. The salts were washed with methylene chloride
(2.times.500 ml). The filtrates were combined and the solvent was
removed in vacuo to produce a yellow oil. The oil was dissolved in
1M aqueous HCl (1500 ml) and washed with ethyl acetate (3.times.500
ml). The aqueous layer was cooled in an ice bath to below 5.degree.
C. and the pH of the aqueous layer was adjusted to 12 to 13 with a
10 M aqueous NaOH solution (220 ml) keeping the internal
temperature at less than or equal to 10.degree. C. The mixture was
allowed to warm to room temperature. The aqueous layer was
extracted with methylene chloride (2.times.500 ml). The organic
layers were combined and washed with brine solution (500 ml), dried
(Na.sub.2SO.sub.4) and the solvent was removed in vacuo to afford a
yellow viscous oil. The yield was 186.4 g (88.5%). .sup.1H NMR was
consistent with Intermediate 3.
Intermediate 4 Dioxalate Salt
(1R,2R)-2-Amino-1-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-3-pyrrolidin-1-yl-
-propan-1-ol dioxalate Salt
[0088] A suspension of Intermediate 3 (358 g, 0.90 moles), ethanol
(1500 ml), 1M HCl solution (1500 ml) and 10% Pd(OH).sub.2 (32 g, 20
weight %) were hydrogenated at about 50 psi for about 36 h at room
temperature. The mixture was filtered through a Cuono filter. The
Cuono filter was washed with 10.degree. % ethanol in water (500
ml). The filtrates were combined and ethanol was removed in vacuo.
The aqueous layer was extracted with ethyl acetate (3.times.600
ml). The organic layer was extracted with 1M HCl aqueous (700 ml).
The aqueous layers were combined and cooled in an ice bath (0 about
5.degree. C.). The pH of the aqueous layer was adjusted to about 12
(pH paper) with 10 M aqueous NaOH solution (490 ml) keeping the
internal temperature below 10.degree. C. The aqueous layer was
allowed to warm to room temperature. The aqueous layer was
extracted with methylene chloride (2.times.1500 ml, 1.times.750
ml). The combined organic layers were dried over MgSO.sub.4 and the
solvent was removed in vacuo to afford a yellow viscous oil. The
crude weight was 214.3 g (86%). .sup.1H NMR was consistent with
Intermediate 4.
[0089] A solution of oxalic acid (152.4 g, 1.693 moles, 2.2
equivalents) in methylisobutyl ketone (2300 ml) was added slowly
with stirring to a solution of Intermediate 4 (214.3 g, 0.77 moles,
1 equivalent) in methylisobutyl ketone (800 ml) at room
temperature. The resulting mixture was stirred at room temperature
for about 2.5 hours. The solid was filtered, and triturated with
acetone (2000 ml) at room temperature for about 16 hours. The solid
was filtered, washed with acetone (3.times.100 ml) and dried under
high vacuum to produce an off-white solid. The yield was 312.5 g
(89%). .sup.1H NMR was consistent with Intermediate 4 dioxalate
salt.
Compound 5
(1R,2R)-Hexadecannoic acid
[2-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-2-hydroxy-1-pyrrolidin-1-ylmeth-
yl-ethyl]-amide
[0090] To a cold solution (about 5.degree. C.) of Intermediate 4
dioxalate salt (507 g, 1.11 moles) in water (10 L) was added a 10 M
aqueous NaOH solution (500 ml) with stirring while keeping the
internal temperature below 10.degree. C. The solution was allowed
to warm to room temperature while the pH of the solution was
maintained at about 14 (pH paper). The aqueous layer was extracted
with methylene chloride (3.times.6000 ml). The organic layers were
combined, washed with water (2000 ml), dried (MgSO.sub.4) and the
solvent was removed in vacuo to afford a yellow viscous oil,
Intermediate 4. The yield was 302 g (98%). .sup.1H NMR was
consistent with Intermediate 4.
[0091] A solution of palmitic acid NHS-ester (Sigma, 382.5 g, 1.01
equivalents) in methylene chloride (2500 ml) was added to a
solution of intermediate 4 (302 g) in methylene chloride (1500 ml)
at room temperature over a period of 1.25 hours under a nitrogen
atmosphere. The mixture was allowed to stir at room temperature for
about 18 hours. A solution of 1M aqueous NaOH (2425 ml) was added
and the mixture was stirred at room temperature for about 3 hours.
The organic layer was separated and the aqueous layer was extracted
with methylene chloride (800 ml). The organic layers were combined,
washed with a 1M NaOH solution (3.times.1500 ml) and water (1500
ml). The organic layer was dried over MgSO.sub.4 and the solvent
was removed in vacuo to afford a semi solid. The semi-solid was
coevaporated with heptane (3.times.100 ml). The crude product was
transferred to a 12 L three-necked RB flask and heptane (7500 ml)
was added. The mixture was heated at reflux with stirring under a
nitrogen atmosphere. The solution was slowly cooled to about
55.degree. C. (internal temperature) and poured into another flask.
The solution was stirred at room temperature for 24 hours under a
nitrogen atmosphere. The off white solid was filtered, washed with
heptane (2.times.500 ml) and dried under high vacuum for 24 hours.
The solid (397 g) was transferred to a 12 L RB flask and 30% ethyl
acetate in heptane (8000 ml) was added. The resulting mixture was
heated at reflux for 30 minutes with stirring. The solution was
cooled to about 55.degree. C. (internal temperature) and poured
into another flask. The stirring was continued at room temperature
under a nitrogen atmosphere for about 24 hours. The solid was
filtered, washed with heptane (2.times.100 ml) and dried under high
vacuum to afford an off white solid. The yield was 324 g (58%).
.sup.1H NMR and TLC were consistent with Compound 5. mp
96.1.degree. C. HPLC analysis: chiral purity 99.7%, chemical purity
99.7%. Anal. Calcd for C.sub.31H.sub.52N.sub.2O.sub.4: C, 72.05; H,
10.14; N, 5.42. Found C, 72.03; H, 10.19; N, 5.42.
Example 4--Preparation of Compounds 6-8
[0092] N-hydroxysuccinimide esters of fatty acids were prepared by
the method of Lapidot, Y. Rappoport, S. and Wolman, Y. Journal of
Lipid Research 8, 1967 or as described below:
Octanoic Acid N-Hydroxysuccinimide Ester
[0093] N-hydroxysuccinimide (Aldrich, 20.0 g, 173 mmol) and
triethyl amine (29 mL, 208 mmol) were dissolved in methylene
chloride in an ice bath under nitrogen. Octanoyl chloride (Aldrich,
35 mL, 205 mmol) was added dropwise over 0.5 hours. The ice bath
was removed and the solution with a white solid was stirred for 1
hour at room temperature. The white solid was removed by filtration
and the filtrate was washed with water (100 mL) and saturated
aqueous sodium bicarbonate (100 mL). The organic layer was dried
with sodium sulfate, filtered and heptane (100 mL) was added. The
solution was rotoevaporated to remove most of the methylene
chloride and leave a colorless to white flaky precipitate in
heptane. The precipitate was filtered and washed with heptane.
After dying. Octanoic acid N-hydroxysuccinimide ester was obtained
in 84% yield (35.4 g): .sup.1H NMR (CDCl.sub.3) 2.84 (br s, 4H),
2.60 (t, J=7.48 Hz, 2H), 1.78-1.71 (m, 2H), 1.42-1.26 (m, 8H), 0.88
(t, J=6.7 Hz, 3H) ppm.
Compound 6
(1R,2R)-Octanoic acid
[2-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-2-hydroxy-1-pyrrolidin-1-ylmeth-
yl-ethyl]-amide
##STR00024##
[0095] To Intermediate 5 (22.36 g& 80.33 mmol) dissolved in
anhydrous methylene chloride (300 mL) was added a solution of
octanoic acid N-hydroxysuccinimide ester (19.4 g, 80.39 mmol)
dissolved in anhydrous methylene chloride (150 mL) over 15-30
minutes under nitrogen at room temperature. The solution was
stirred at room temperature for 18-20 hours. To the reaction was
added 1 M aqueous NaOH solution (200 mL). The two phase system was
stirred at room temperature for 45 minutes. The layers were
separated and the combined organic layers were washed twice with 1M
NaOH (2.times.200 mL) and twice with water (2.times.100 mL). The
organic layer was dried with sodium sulfate, filtered and
rotoevaporated to a yellow oil. Most of the crude material was
dissolved in 5% ethyl acetate in heptane (1 L) at reflux. After
cooling to 40.degree. C., the hazy solution was separated from the
yellow oil by decanting the solution into a new flask. The first
flask was rinsed twice with 5% ethyl acetate in heptane
(2.times.250 mL) by the same process (reflux and cooling to
40.degree. C. and decanting the solution from the oil). The
combined solution was heated to reflux and allowed to cool to room
temperature over 4 hours. The resulting white solid was filtered
and washed with 5% ethyl acetate in heptane (100 mL) and heptane
(100 mL). The white solid (13.9 g) was dried under vacuum for 16-24
hours. This solid was mostly dissolved in 5% ethyl acetate in
heptane (800 mL) at reflux. After cooling to 50.degree. C., the
hazy solution was separated from the yellow oil by decanting the
solution into a new flask. The first flask was rinsed with 5% ethyl
acetate in heptane (100 mL) by the same process (reflux and cooling
to 50.degree. C. and decanting the solution from the oil). The
combined solution was heated to reflux and allowed to cool to room
temperature over 4 hours. The resulting white solid was filtered
and washed with 5% ethyl acetate/heptane (50 mL) and heptane (50
mL). After drying at room temperature under vacuum for 2-3 days,
Compound 6 was obtained in 39% yield (12.57 g). Analytical chiral
HPLC (column: Chirex (S)-VAL and (R)-NE, 4.6.times.250 mm) showed
this material to be 99.9% the desired R,R isomer. Analytical HPLC
showed this material to be 99.6% pure. mp 87-88.degree. C. .sup.1H
NMR (CDCl.sub.3) .delta. 6.86-6.73 (m, 3H), 5.84 (d, J=7.3 Hz, 1H),
4.91 (d, J=3.4 Hz, 1H), 4.25 (s, 4H), 4.24-4.18 (m, 1H), 2.85-2.75
(m, 2H), 2.69-2.62 (m, 4H), 2.10 (t, J=7.3 Hz, 2H), 1.55-1.45 (m,
2H), 1.70-1.85 (m, 4H), 1.30-1.15 (m, 8H), 0.87 (t, J=6.9 Hz, 3H)
ppm.
Compound 7
(1R,2R)-Nonanoic acid
[2-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-2-hydroxy-1-pyrrolin-1-ylmethyl-
-ethyl]-amide
##STR00025##
[0097] This compound was prepared by the method described for
Compound 6 using Nonanoic acid N-hydroxysuccinimide ester.
Analytical HPLC showed this material to be 98.4% pure. mp
74-75.degree. C. .sup.1H NMR (CDCl.sub.3) .delta. 6.86-6.76 (m,
3H), 5.83 (d, J=7.3 Hz, 1H), 4.90 (d, J=3.3 Hz, 1H), 4.24 (a, 4H),
4.24-4.18 (an, 1H), 2.85-2.75 (m, 2H), 2.69-2.62 (m, 4H), 2.10 (t,
J=7.3 Hz, 2H), 1.55-1.45 (m, 2H), 1.70-1.85 (m, 4H), 1.30-1.15 (m,
10H), 0.87 (t, J=6.9 Hz, 3H) ppm.
Compound 8
(1R,2R)-Decanoic
[2-(2',3'-dihydro-benzo[1,4]dioxin-6'-yl)-2-hydroxy-1-pyrrolidin-1-ylmeth-
yl-ethyl]-amide
##STR00026##
[0099] This compound was prepared by the method described for
Compound 6 using decanoic acid N-hydroxysuccinimide ester.
Analytical HPLC showed this material to be 99.3% pure. mp
97.5-98.5.degree. C. .sup.1H NMR (CDCl.sub.3) .delta. 6.86-6.76 (m,
3H), 3.83 (d, J=7.5 Hz, 1H), 4.90 (d, J=3.4 Hz, 1H), 4.24 (s, 4H),
4.24-4.18 (m, 1H), 2.85-2.75 (m, 2H), 2.69-2.62 (m, 4H), 2.10 (t,
J=7.5 Hz, 2H), 1.55-1.45 (m, 2H), 1.70-1.85 (m, 4H), 1.30-1.15 (m,
12H), 0.87 (t, J=6.8 Hz, 3H) ppm.
Example 5--Preparation of Compound 13
Intermediate 9
(1R,3S,5S,8aS)-1,3-Bis-4-benzyloxy-phenyl)-5-phenyl-tetrahydro-oxazolo[4,3-
-c][1,4]oxazin-8-one
##STR00027##
[0101] The (5S)-5-phenylmorpholin-2-one.HCl salt (57.45, 268.9
mmol) was stirred with ethyl acetate (500 mL) and saturated aqueous
sodium bicarbonate (250 mL) for 30 minutes, until the biphasic
solution was clear. The phases were separated, and the aqueous
layer was extracted with ethyl acetate (2.times.250 mL). The
combined organic phases were washed with saturated sodium chloride
solution (250 mL). The organic layer was dried with sodium sulfate,
filtered, concentrated to an oil, and dried under vacuum for 60
minutes. The 5-(S)-phenyl morpholin-2-one was obtained in a 86%
yield (40.98 g, 231.3 mmol).
[0102] The 5-(S)-phenyl morpholin-2-one (40.98 g, 231.3 mmol) and
4-benzyloxybenzaldehyde (Aldrich, 147.3 g, 694 mmol, 3.0
equivalents) was dissolved in toluene (750 mL). The reaction was
fitted with a Dean Stark Trap and a reflux condenser. The solution
was refluxed under nitrogen for 2 days. After cooling to room
temperature, the solvent was removed by rotoevaporation and the oil
was dissolved in ethyl acetate (500 mL). A solution of sodium
bisulfite (Aldrich, 125 g) dissolved in water (250 mL) was added
and the two phase mixture was stirred at room temperature for 3
hours. The resulting white solid was filtered off and washed with
ethyl acetate. The filtrate was placed in a separatory funnel and
the layers separated. The organic layer was washed with water (250
mL), saturated aqueous sodium chloride solution (250 mL) and then
dried (sodium sulfate) filtered and rotoevaporated to a foamy oil
(144 g). After drying under vacuum for 1 hour, tert-butyl methyl
ether (1450 mL) was added and the mixture was stirred at room
temperature for 5 hours. The resulting white-yellow solid was
filtered. The solid was dried under vacuum. Intermediate 9 was
obtained in 27% yield (41.64 g, 71.46 mmol). .sup.1H NMR
(CDCl.sub.3) .delta. 7.5-6.8 (m, 23H), 5.0 and 5.1 (2 s, 4H),
4.5-4.3 (m, 2H), 4.2-4.1 (m, 2H) ppm.
Intermediate 10
(2S,3R,1''S)-3-(4-Benzyloxy-phenyl)-3-hydroxy-2-(2''-hydroxy-1''-phenyl-et-
hylamino)-1-pyrrolidin-1-yl-propan-1-one
##STR00028##
[0104] To Intermediate 9 (45.1 g, 77.4 mmol) dissolved in
tetrahydrofuran (250 mL) was added pyrrolidine (Aldrich 33 mL, 395
mmol, 5.1 equivalents). The solution was stirred capped under
nitrogen at room temperature for 16-18 hours. The solvent was
rotoevaporated to yield a yellow foamy oil which was vacuum dried
for 0.5 hours. The crude was dissolved in methanol (220 mL) and a
1M aqueous HCl solution (220 mL) was added. The solution was
refluxed for 4 hours. After cooling to room temperature, the
methanol was removed by rotoevaporation. To the resulting oil was
slowly added 10 M aqueous NaOH (22 mL to adjust the pH to 14). The
product was extracted three times with methylene chloride
(300,100,100 mL) from the basic aqueous layer. After drying with
sodium sulfate the combined organic layer was filtered and
rotoevaporated to yield a yellow-orange foamy solid. Tert-butyl
methyl ether (300 mL) was added and the mixture was stirred at room
temperature for 7 hours. The resulting white-yellow solid was
filtered, washed with tert-butyl methyl ether (50 mL) and vacuum
dried. Intermediate 10 was obtained in 83% yield (29.77 g). .sup.1H
NMR (CDCl.sub.3) .delta. 7.4-7.2 (m, 12H), 6.9-6.8 (m, 2H), 5.05
(AB quartet, 2H), 4.47 (d, J=8.5, 1H), 3.9-3.3 (m, 3H), 3.015 (d,
J=8.5, 1H), 3.0-2.8 (m, 2H), 2.3-2.2 (m, 1H), 1.85-1.7 (m, 1H),
1.45-1.15 (m, 4H) ppm.
Intermediate 11
(1R,2R,1''S)-1-(4-Benzyloxy-phenyl)-2-(2''-hydroxy-''-phenyl-by-ethylamino-
)-3-pyrrolidin-1-yl-propan-1-ol
##STR00029##
[0106] In a 3-neck flask with dropping funnel and condenser under
nitrogen was added LiAlH.sub.4 (Aldrich, 6.3 g, 166 mmol, 2.57
equivalents) and anhydrous tetrahydrofuran (75 mL). A solution of
Intermediate 10 (29.7 g, 64.48 mmol) in anhydrous tetrahydrofuran
(300 mL) was added dropwise to the reaction over 15-30 minutes. The
reaction was refluxed under nitrogen for 9 hours. The reaction was
cooled in an ice bath and water (7.0 mL) was very carefully added
drop by drop (vigorous exothermic reaction with hydrogen being
given off). A 15% aqueous NaOH solution (7.0 mL) was added dropwise
followed by water (21 mL). Halfway through the final water addition
a large amount of a white solid formed. It was broken up by the
addition of methylene chloride (250 mL). After stirring at room
temperature for 15 minutes, the mixture was filtered through a
celite plug (17 cm in diameter by 1 cm in height). The precipitate
was washed with methylene chloride (2.times.250 mL). The filtrate
was rotoevporated to an oil. The oil was dissolved in 1M aqueous
HCl (300 mL). This aqueous layer was washed with tert-butyl methyl
ether (2.times.200 mL). After cooling in an ice bath, 10 M aqueous
NaOH (35 mL) was carefully added to the aqueous layer (final
pH=14). The product was extracted three times with methylene
chloride (300 mL, 200 mL and 100 mL). After drying with sodium
sulfate, the solution was filtered and rotoevaporated to yield a
white solid. After drying, the Intermediate 1 was obtained in 94%
yield (26.9 g). .sup.1H NMR (CDCl.sub.3) .delta. 7.46-7.115 (m,
12H), 6.98-6.96 (m, 2H), 5.08 (s, 2H), 4.49 (d, J=4.7, 1H),
3.70-3.65 (m, 1H), 3.60-3.55 (m, 1H), 3.54-3.45 (m, 1H), 3.00-2.90
(m, 1H), 2.7-2.6 (m, 1H), 2.36 (br s, 4H), 2.15-2.05 (m, 1H), 1.70
(br s, 4H) ppm.
Intermediate 12
(1R,2R)-2-Amino-1-(4-benzyloxy-phenyl)-3-pyrrolidin-1-yl-propan-1-ol
Hydrogen Chloride Salt
##STR00030##
[0108] Intermediate 11 (26.9 g, 60.24 mmol) was dissolved in
methanol (400 mL) and 1M aqueous HCl (130 mL) was added. After
being placed under nitrogen, 20% palladium hydroxide on carbon
(Pearlman's catalysis, Aldrich, 10.8 g) was added. The reaction was
placed under nitrogen and then under hydrogen by evacuation and
filling to a balloon. The mixture was stirred for 48 hours at room
temperature under a hydrogen balloon. The reaction was placed under
nitrogen and filtered through a pad of celite. The celite pad was
washed with 10% water in methanol (250 mL) and water (50 mL). The
solvent was removed by rotoevaporation and coevaporation with
toluene (3.times.100 mL). The foamy solid was dissolved in
isopropanol (300 mL) at reflux. The solution was cooled to room
temperature and tert-butyl methyl ether (550 mL) was added. After
stirring at room temperature for 2 hours, the white solid was
filtered and washed with tert-butyl methyl ether. After drying,
Intermediate 12 was obtained in ca 99% yield (18 g). .sup.1H NMR
(DMSO-d6) .delta. 9.68 (br s, 1H), 8.53 (br s, 2H) 7.24 (d, J=8.55
Hz, 2H), 6.80 (d, J=8.55 Hz, 2H), 4.72 (d, J=7.0 Hz, 1H), 3.8-3.6
(m, 2H), 3.4-3.6 (m, 3H), 3.0-3.2 (m, 2H), 2.7-2.5 (br s, 1H),
2.0-1.7 (br s, 4H) ppm.
Compound 13
(1R,2R)-Hexadecanoic acid
[2-(4-benzyloxy-phenyl)-2-hydroxy-1-pyrrolidin-1-ylmethyl-ethyl]-amide
##STR00031##
[0110] To Intermediate 12 (16.17 g 49.36 mmol) suspended in
tetrahydrofuran (500 mL) was added triethylamine (28 mL, 4
equivalents). A solution of Palmitic acid N-hydroxysuccinimide
ester (Sigma, 19.2 g, 54.29 mmol) dissolved in tetrahydrofuran (125
mL) was added over 30 minutes under nitrogen at room temperature.
The solution was stirred at room temperature for 18-20 hours. The
white precipitate was removed by filtration and the filtrate was
rotoevaporated to a foamy off-white solid (35.5 g). The crude
material was dissolved in methylene chloride (500 mL) and washed
with water (100 mL) and saturated aqueous sodium carbonate solution
(100 mL). After drying with sodium sulfate, the solution was
filtered and rotoevaporated to yield a off-white foamy solid (24.75
g). This material was recrystallized from 40% ethyl acetate in
heptane (500 mL, hot filtration). Compound 13 was obtained in 61%
yield (14.45 g) Analytical chiral HPLC showed this material to be
99.7% the desired R,R isomer. Analytical HPLC showed this material
to be 99.6% pure. mp 95-97.degree. C. .sup.1H NMR (CDCl.sub.3)
.delta. 7.15 (d, J=8.5 Hz, 2H), 6.70 (d, J=8.5 Hz, 2H), 6.0 (d,
J=7.3, 1H), 4.96 (d, J=3.8, 1H), 4.3-4.2 (m, 1H), 2.9-2.7 (m, 2H),
2.65-2.55 (m, 4H), 2.10 (t, J=7.5, 2H), 1.75 (br s, 4H), 1.58-1.46
(m, 2H), 1.32-1.16 (m, 24H), 0.9 (t, J=6.7, 3H) ppm.
[0111] While this invention has been particularly shown and
described with references to preferred embodiments thereof, it will
be understood by those skilled in the art that various changes in
form and details may be made therein without departing from the
scope of the invention encompassed by the appended claims.
* * * * *