U.S. patent application number 15/506820 was filed with the patent office on 2017-09-28 for analyzing device.
This patent application is currently assigned to SHIMADZU CORPORATION. The applicant listed for this patent is SHIMADZU CORPORATION. Invention is credited to Yasuhiro FUNADA.
Application Number | 20170276652 15/506820 |
Document ID | / |
Family ID | 55398946 |
Filed Date | 2017-09-28 |
United States Patent
Application |
20170276652 |
Kind Code |
A1 |
FUNADA; Yasuhiro |
September 28, 2017 |
ANALYZING DEVICE
Abstract
An analyzing device includes a splitting part for causing fluid
containing a sample component to flow separately in a first flow
passage and a second flow passage; an analyzing column provided on
the first flow passage for separating the sample component from the
fluid; a first back pressure regulating valve corresponding to a
first pressure controlling unit for controlling a pressure in the
first flow passage; and a second back pressure regulating valve
corresponding to a second pressure controlling unit for controlling
a pressure in the second flow passage, wherein flow rate of the
fluid in the first flow passage and flow rate of the fluid in the
second flow passage are controlled based on a ratio of the pressure
in the first flow passage to the pressure in the second flow
passage.
Inventors: |
FUNADA; Yasuhiro;
(Kyoto-shi, JP) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
SHIMADZU CORPORATION |
Kyoto-shi, Kyoto |
|
JP |
|
|
Assignee: |
SHIMADZU CORPORATION
Kyoto-shi, Kyoto
JP
|
Family ID: |
55398946 |
Appl. No.: |
15/506820 |
Filed: |
August 28, 2014 |
PCT Filed: |
August 28, 2014 |
PCT NO: |
PCT/JP2014/072533 |
371 Date: |
February 27, 2017 |
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
G01N 30/32 20130101;
G01N 30/88 20130101; G01N 2030/328 20130101; G01N 30/8651 20130101;
G01N 25/14 20130101; G01N 2030/324 20130101; B01D 15/40 20130101;
G06G 7/75 20130101; G01N 30/36 20130101; G01N 30/80 20130101; B01D
15/40 20130101 |
International
Class: |
G01N 30/36 20060101
G01N030/36; G01N 30/88 20060101 G01N030/88; G01N 30/32 20060101
G01N030/32; G01N 25/14 20060101 G01N025/14; G01N 30/86 20060101
G01N030/86; G06G 7/75 20060101 G06G007/75 |
Claims
1. An analyzing device comprising: a) a splitting part for causing
supercritical fluid containing a sample component to flow
separately in a first flow passage and a second flow passage; b) a
column provided on the first flow passage for separating the sample
component from the supercritical fluid; c) a first pressure
controlling unit for controlling a pressure in the first flow
passage; d) a second pressure controlling unit for controlling a
pressure in the second flow passage; and flow rate of the
supercritical fluid in the first flow passage and flow rate of the
supercritical fluid in the second flow passage are controlled based
on a ratio of the pressure in the first flow passage to the
pressure in the second flow passage.
2. (canceled)
3. The analyzing device according to claim 1, wherein a sum of
values of the pressure in the first flow passage and the pressure
in the second flow passage is a value of a pressure exceeding a
critical pressure of the supercritical fluid.
4. The analyzing device according to claim 1, wherein the first
pressure controlling unit has a first pressure controlling valve
provided on the first flow passage, and the second pressure
controlling unit has a second pressure controlling valve provided
on the second flow passage.
5. (canceled)
6. (canceled)
7. An analyzing method comprising the steps of: a) controlling a
pressure in a first flow passage by a first pressure controlling
unit; b) controlling a pressure in a second flow passage by a
second pressure controlling unit; c) controlling flow rate of
supercritical fluid containing a sample component in the first flow
passage and flow rate of supercritical fluid containing a sample in
the second flow passage based on a ratio of the pressure in the
first flow passage to the pressure in the second flow passage; d)
splitting the supercritical fluid to flow separately in the first
flow passage and the second flow passage by means of a splitting
part; and e) separating the sample component from the supercritical
fluid using a column provided on the first flow passage, wherein an
extracting unit that outputs the supercritical fluid containing the
sample component is connected upstream of the splitting part.
8. (canceled)
9. The analyzing method according to claim 7, wherein a sum of
values of the pressure in the first flow passage and the pressure
in the second flow passage is a value of a pressure exceeding a
critical pressure of the supercritical fluid.
10. The analyzing method according to claim 7, wherein the first
pressure controlling unit has a first pressure controlling valve
provided on the first flow passage, and the second pressure
controlling unit has a second pressure controlling valve provided
on the second flow passage.
11. (canceled)
12. (canceled)
Description
TECHNICAL FIELD
[0001] The present invention relates to an analyzing device that
separates a plurality of components contained in a sample into the
individual components, and specifically, relates to a chromatograph
using supercritical fluid.
BACKGROUND ART
[0002] Supercritical fluid can be realized by holding fluid that is
gas at normal temperature and normal pressure (for example, carbon
dioxide) at higher temperature and pressure than those at the
critical point of the fluid (in the case of carbon dioxide, the
critical temperature is 31.degree. C. and the critical pressure is
7.4 MPa). The supercritical fluid exhibits excellent ability as
solvent to many substances, and is often used for supercritical
fluid extraction (hereinafter referred to as SFE) and supercritical
fluid chromatography (hereinafter referred to as SFC).
[0003] Patent Literature 1 discloses an extraction and
separation/analyzing device using supercritical fluid which can
perform any of SFE and SFC by switching between a flow system for
SFE and a flow system for SFC with a switching valve. With separate
SFE and SFC flow systems, a component extracted from a sample by
SFE is first trapped in a trap column, and then, is eluted with a
solvent. Therefore, in order to measure the SFE-extracted
substances by the chromatograph, human intervention is needed for
adjusting the concentration of the extracted substance and placing
it in a sample introducing unit of the chromatograph. As such, with
an off-line configuration in which extracted substances are not
directly introduced into a column, a series of operations from
extraction to analysis cannot be automatically performed.
[0004] Patent Literature 2 discloses an analyzing device using
supercritical fluid which has an on-line configuration in which SFE
and SFC are integrated into one flow system, and is capable of
automatically performing a series of operations from extraction to
analysis. An example of such an analyzing device with the on-line
configuration is shown in FIG. 3.
[0005] The analyzing device 30 of FIG. 3 is configured of a
cylinder 300, a pressurizing pump 301, a solvent container 302, a
modifier pump 303, a first flow passage switching valve 304, a
sample storing container 305, a temperature adjusting device 306, a
needle 307, a second flow passage switching valve 308, an analyzing
column 309, an ultraviolet detector (UV) 310, a back pressure
regulating valve 311 and a mass spectrometer (MS) 312.
[0006] Carbon dioxide that is drawn out of the cylinder 300 by the
pressurizing pump 301 and pressurized (to supercritical fluid) is
sent, via the first flow passage switching valve 304 along with a
modifier agent drawn out of the solvent container 302, to the
sample storing container 305 which is temperature controllable by
the temperature adjusting device 306. By the pressurizing pump 301
and the later-mentioned back pressure regulating valve 311, the
pressure of the flow passage between them is set to a pressure
exceeding the critical pressure, and the temperature of the sample
storing container 305 is set to a temperature exceeding the
critical temperature by the temperature adjusting device 306.
Thereby, carbon dioxide is put into supercritical state inside the
sample storing container 305, and the supercritical carbon dioxide,
with its excellent capability as solvent, extract components from a
sample in the sample storing container 305 (SFE).
[0007] The supercritical fluid containing the SFE-extracted
components flows into the analyzing column 309 from the needle 307
attached to the sample storing container 305 via the second flow
passage switching valve 308. The supercritical fluid containing the
SFE-extracted components is separated to individual components by
the analyzing column 309, and then, flows through the ultraviolet
detector (UV) 310, the back pressure regulating valve 311 and the
mass spectrometer (MS) 312, where the individual components are
analyzed (SFC).
CITATION LIST
Patent Literature
[0008] [Patent Literature 1] JP 4-332863 A
[0009] [Patent Literature 2] JP 60-8747 A
SUMMARY OF INVENTION
Technical Problem
[0010] When an agricultural product is analyzed as a sample by a
chromatograph to measure the amount of pesticide residues in the
agricultural product using the conventional analyzing device 30, a
large amount of substances that constitute noise to the analysis of
the pesticide, such as pigments, lipids and saccharides in the
agricultural product itself, are extracted in addition to the
target component in the SFE-extracted components. With the
configuration of the conventional analyzing device 30, when such a
sample is analyzed, the entirety of the SFE-extracted components is
introduced into the analyzing column 309. Consequently, the
analyzing column 309 tends to deteriorate due to the large amount
of unwanted constituents, and saturation of the ultraviolet
detector (UV) 310 and contamination of the mass spectrometer (MS)
312 tend to arise. As such, for some samples, the analyzing device
30 may be burdened or correct measurement may be difficult, which
narrows the range of analyzable samples of the analyzing device 30
and lessens its versatility.
[0011] A problem to be solved by the present invention is to
provide a highly versatile analyzing device that widens the range
of analyzable samples.
Solution to Problem
[0012] An analyzing device according to the present invention
devised to solve the aforementioned problem includes:
[0013] a) a splitting part for causing fluid containing a sample
component to flow separately in a first flow passage and a second
flow passage;
[0014] b) a column provided on the first flow passage for
separating the sample component from the fluid;
[0015] c) a first pressure controlling unit for controlling a
pressure in the first flow passage; and
[0016] d) a second pressure controlling unit for controlling a
pressure in the second flow passage, wherein
[0017] flow rate of the fluid in the first flow passage and flow
rate of the fluid in the second flow passage are controlled based
on a ratio of the pressure in the first flow passage to the
pressure in the second flow passage.
[0018] Moreover, an analyzing method according to the present
invention devised to solve the aforementioned problem includes the
steps of:
[0019] a) controlling a pressure in a first flow passage by a first
pressure controlling unit;
[0020] b) controlling a pressure in a second flow passage by a
second pressure controlling unit;
[0021] c) controlling flow rate of fluid containing a sample
component in the first flow passage and flow rate of fluid
containing a sample component in the second flow passage based on a
ratio of the pressure in the first flow passage to the pressure in
the second flow passage;
[0022] d) splitting the fluid to flow separately in the first flow
passage and the second flow passage by means of a splitting part;
and
[0023] e) separating the sample component from the fluid using a
column provided on the first flow passage.
[0024] The "fluid" is preferably supercritical fluid, but it is not
limited to this, and it may be gas or liquid. When the fluid is
supercritical fluid, the pressure in the first flow passage and the
pressure in the second flow passage are set such that a sum of
values of these pressures is a value of a pressure exceeding a
critical pressure of the fluid.
[0025] Both of the first pressure controlling unit and the second
pressure controlling unit may have pressure controlling valves, and
the pressure controlling valves may be provided on the first flow
passage and the second flow passage respectively.
[0026] Upstream of the splitting part, an extracting unit (SFE
Unit) for performing extraction using supercritical fluid (SFE) may
be connected, or a sample injecting unit (auto-sampler) may be
connected. In other words, there may be applied an on-line
configuration in which SFE and SFC are integrated into one flow
system or a configuration in which only chromatography using
supercritical fluid (SFC) can be performed.
Advantageous Effects of Invention
[0027] According to the analyzing device and the analyzing method
according to the present invention having the aforementioned
configurations, by changing the ratio of the pressure in the first
flow passage to the pressure in the second flow passage using the
first pressure controlling unit and the second pressure controlling
unit, the flow rate of the fluid in the first flow passage and flow
rate of the fluid in the second flow passage after split at the
splitting part can be controlled. When a large amount of unwanted
constituents for analysis are contained in the fluid containing the
sample components, the flow rate of the fluid in the column
provided on the first flow passage is reduced such that the
entirety of the unwanted constituents is not introduced into the
column. This can suppress deterioration of the column and can
reduce burden on the analyzing device. In this way, a highly
versatile analyzing device that can widen the range of analyzable
samples can be provided.
BRIEF DESCRIPTION OF DRAWINGS
[0028] FIG. 1 is a schematic configuration diagram of an analyzing
device according to a first embodiment of the present
invention.
[0029] FIG. 2 is a schematic configuration diagram of an analyzing
device according to a second embodiment of the present
invention.
[0030] FIG. 3 is a schematic configuration diagram of a
conventional analyzing device.
DESCRIPTION OF EMBODIMENTS
[0031] Hereafter, modes for implementing the present invention are
described with reference to embodiments.
First Embodiment
[0032] FIG. 1 is a schematic configuration diagram of an analyzing
device of a first embodiment. The analyzing device 10 of this
embodiment is configured of a cylinder 100, a pressurizing pump
101, a solvent container 102, a modifier pump 103, an extracting
unit 114, an analyzing column 109, a splitting part 110, a first
back pressure regulating valve 111, a second back pressure
regulating valve 112, a mass spectrometer (MS) 113 and a recovering
container 115. The extracting unit 114 is configured of a first
flow passage switching valve 104, a sample storing container 105, a
temperature adjusting device 106, a needle 107, a second flow
passage switching valve 108, and pipes connecting these.
[0033] The analyzing device 10 of this embodiment has a feature of
including the splitting part 110, the first back pressure
regulating valve 111 and the second back pressure regulating valve
112. Fluid containing sample components flows in pipes separately
in two flow passages of a first flow passage and a second flow
passage by means of the splitting part 110. This embodiment has an
on-line configuration in which the extracting unit 114 is directly
connected upstream of the splitting part 110, and the analyzing
column 109 is directly connected to the first flow passage
downstream thereof Hereafter, description will be made to a case
where the extracting unit 114 extracts pesticide residues contained
in a sample (agricultural product) by SFE using carbon dioxide as
supercritical fluid, and the analyzing column 109 separates
components in the pesticide residues. The first back pressure
regulating valve 111 and the second back pressure regulating valve
112 that are pressure controlling valves correspond to a first
pressure controlling unit and a second pressure controlling unit,
respectively. The analyzing device 10 operates as follows to
separate a plurality of components contained in extracted
substances by the extracting unit 114 into the individual
components using the analyzing column 109, thereby to identify the
individual components by the mass spectrometer 113.
[0034] First, an agricultural product as a sample is put in the
sample storing container 105, to one end of which the needle 107 is
attached. These operations may be performed by a user or may be
performed by a not-shown controlling device. In this way, the
individual parts are connected as in FIG. 1. One or plurality of
sample storing container s105 may be prepared as in FIG. 1, and a
configuration may be adopted in which extraction for a plurality of
samples can be performed.
[0035] Next, carbon dioxide (supercritical fluid) is drawn out of
the cylinder 100 under pressurization by the pressurizing pump 101.
Also, a modifier agent that is a polar solvent (methanol, ethanol
or the like) is drawn out of the solvent container 102 by the
modifier pump 103. These are sent, via the first flow passage
switching valve 104, to the sample storing container 105 which is
temperature controllable by the temperature adjusting device 106
such as a heater. When the pressure in a flow passage between the
pressurizing pump 101 and the splitting part 110 is set to be a
pressure exceeding the critical pressure (7.4 MPa) of carbon
dioxide by the pressurizing pump 101 and the later-mentioned first
back pressure regulating valve 111 and second back pressure
regulating valve 112, and the sample storing container 105 is set
to have a temperature exceeding the critical temperature
(31.degree. C.) of carbon dioxide by the temperature adjusting
device 106, carbon dioxide is in the supercritical state
(supercritical fluid) inside the sample storing container 105.
Since carbon dioxide in the supercritical state exhibits excellent
ability as solvent, it dissolves the sample (agricultural product)
in the sample storing container 105. Thereby, in addition to
pesticide residues that are the target components in the sample, a
large amount of substances that constitute noise to analysis of the
pesticide residues, such as pigments, lipids and saccharides, are
extracted (SFE).
[0036] The supercritical fluid containing the SFE-extracted
components reaches the splitting part 110 from the needle 107 via
the second flow passage switching valve 108, and splits thereat
into the first flow passage and the second flow passage. The
analyzing column 109 heated at a temperature exceeding the critical
temperature by a not-shown column oven is provided on the first
flow passage, and thus the supercritical fluid containing the
SFE-extracted components and flowing in the first flow passage is
in the supercritical state inside the analyzing column 109. After
the components are separated thereat into individual components,
the supercritical fluid flows in the first back pressure regulating
valve 111 and the mass spectrometer (MS) 113, where the individual
components are analyzed (SFC). In this configuration, a detector
(ultraviolet detector (UV) 310 or the like) (not shown) may be
provided between the analyzing column 109 and the first back
pressure regulating valve 111, and the mass spectrometer 113 is not
essential. After the supercritical fluid containing the
SFE-extracted components and flowing in the second flow passage is
released from the supercritical state by flowing in the second back
pressure regulating valve 112, it is recovered by the recovering
container 115.
[0037] Description will now be made to a case where structures
(sectional shapes and sizes of sectional areas) and materials of
the pipes constituting the first flow passage and the second flow
passage are substantially the same and no difference occurs in
resistances of the fluid due to them. In the case where the pipes
constituting the first flow passage and the second flow passage
have substantially the same structure, and the resistance of the
analyzing column 109 is eliminated, flow rate of fluid in the first
flow passage and flow rate of fluid in the second flow passage are
determined by a split ratio depending on the ratio of the pressures
in these flow passages.
[0038] Assume that the pressure in the first flow passage
controlled by the first back pressure regulating valve 111 is
P.sub.1, the pressure in the second flow passage controlled by the
second back pressure regulating valve 112 is P.sub.2, and the
resistance of the analyzing column 109 is eliminated for
convenience of description. When the flow rate upstream of the
splitting part 110 is, for example, 10 mL/min, the split ratio of
the first flow passage to the second flow passage can be set to be
1:99, that is, P.sub.1=99P.sub.2, and most of the fluid flows in
the second flow passage at low pressure, and the flow rate of the
fluid in the analyzing column 109 reaches 0.1 mL/min. In reality,
although the resistance of the analyzing column 109 cannot be
ignored, the values of the pressures P.sub.1 and P.sub.2 which
realize a desired split ratio can be grasped by investigating
relation between the ratio of the pressures P1 to P2 and the flow
rate in advance thorough a preliminary experiment. When using
supercritical fluid, the sum of the values of the pressure P.sub.1
in the first flow passage and the pressure P.sub.2 in the second
flow passage should be set to be the value of a pressure exceeding
the critical pressure of the fluid.
[0039] By changing the ratio of the pressure P.sub.1 in the first
flow passage to the pressure P.sub.2 in the second flow passage
using the first back pressure regulating valve 111 and the second
back pressure regulating valve 112 as above, the flow rate of the
fluid in the first flow passage and the flow rate of the fluid in
the second flow passage after split at the splitting part 110 can
be controlled. When a large amount of substances that constitute
noise to analysis are contained in the fluid containing the sample
components, the flow rate of the fluid in the analyzing column 109
provided on the first constitute noise to the is reduced as in the
aforementioned example of the split ratio 1:99 in order to prevent
the entirety of the fluid from being introduced into the analysing
column 109. This supresses, deterioration of the analyzing column
109 and reduces burden on the analyzing device 10. In this way,
even a sample from which a large amount of substances that are
unwanted constituents can be an analysis target, which can enhance
versatility of the analyzing device.
[0040] In the conventional on-line configuration shown in FIG. 3 in
which SFE and SFC are integrated into one flow system, while it is
desired for SFE to increase the flow rate in the sample storing
container 305 for prioritizing the speed for extraction, it is
desired for SFC to reduce the flow rate in the analyzing column 309
for prioritizing separation using the analyzing column 309. In
other words, since SFE and SFC have a trade-off relationship for
the flow rates, extraction time has had to be sacrificed to
prioritize precision of separation by SFC, or the precision of
separation has had to be sacrificed to prioritize the speed of
extraction by SFE, or an intermediate flow rate has had to be set
such that both of the extraction time and the precision of
separation were sacrificed in some extent. Therefore, there has
been conventionally a case where since the flow rate of the fluid
containing the sample components and flowing in the analyzing
column 309 is larger than that suitable for SFC, the fluid has
passed through the analyzing column 309 without sufficient
separation of the sample components, which causes peak broadening
(causes broad peaks). When using supercritical fluid, its excellent
ability as solvent sometimes exceed the trapping ability of the
analyzing column 309, which further causes broad peaks. Such broad
peaks lead to insufficient separation of the components, which
interrupts accurate analysis.
[0041] On the contrary, in the configuration of this embodiment,
the flow rate of the fluid in the analyzing column 109 can be
changed by changing the ratio of the pressure P.sub.1 in the first
flow passage to the pressure P.sub.2 in the second flow passage.
Therefore, in the case of employing the configuration in which the
extracting unit 114 for performing extraction using supercritical
fluid (SFE) is provided upstream of the splitting part 110, the
flow rate in the analyzing column 109 provided on the first flow
passage can be reduced while the flow rate in the sample storing
container 105 in the extracting unit 114 is increased, which can
realize the flow rates respectively suitable for SFE and SFC.
Thereby, in an on-line configuration in which SFE and SFC are
integrated into one flow system, accurate analysis in which the
sample components are sufficiently separated in the analyzing
column 109 and broad peaks are reduced is possible.
[0042] Moreover, because a portion of the fluid containing
SFE-extracted components is recovered into the recovering container
115 in an on-line configuration, the portion can be subjected to
subsequent analysis using another analyzing device as in the case
of an off-line configuration.
[0043] In this embodiment, although the example is described in
which the first back pressure regulating valve 111 and the second
back pressure regulating valve 112 correspond to the first pressure
controlling unit and the second pressure controlling unit,
respectively, and have the same piping structures, it is not
limited to this, and a mechanism in which structures (sectional
shapes and sizes of sectional areas) of pipes constituting the
first flow passage and the second flow passage are different from
each other may be used for the first pressure controlling unit and
the second pressure controlling unit.
Second Embodiment
[0044] FIG. 2 is a schematic configuration diagram of an analyzing
device of a second embodiment. The analyzing device 20 of this
embodiment includes a third flow passage switching valve 21 and an
autosampler 22 in addition to the similar configuration to that of
the analyzing device 10 of the first embodiment including the
cylinder 100, the pressurizing pump 101, the solvent container 102,
the modifier pump 103, the extracting unit 114, the analyzing
column 109, the splitting part 110, the first back pressure
regulating valve 111, the second back pressure regulating valve
112, the mass spectrometer (MS) 113 and the recovering container
115.
[0045] In this embodiment, the third flow passage switching valve
21 is connected upstream of the splitting part 110, and the
splitting part 110 is connected to the extracting unit 114 or the
autosampler 22 by changing connections between ports in the valve
21 as shown in dotted lines or solid lines. The autosampler 22
corresponds to a sample injecting unit. A configuration in which
the splitting part 110 is connected to is the same with that in the
first embodiment, and hereafter, description will be made to a
configuration in which the splitting part 110 is connected to the
autosampler 22 and only chromatography (SFC) using supercritical
fluid can be performed.
[0046] Under pressurization by the pressurizing pump 101, carbon
dioxide (supercritical fluid) is drawn out of the cylinder 100.
Also, a modifier agent that is a polar solvent (methanol, ethanol
or the like) is drawn out of the solvent container 102 by the
modifier pump 103. They are sent to the autosampler 22 as a mobile
phase (fluid) through the third flow passage switching valve 21
provided between the pressurizing pump 101 and the modifier pump
103, and the first flow passage switching valve 104, and a sample
is injected in the autosampler 22. The sample injected by the
autosampler 22 reaches the splitting part 110 again via the third
flow passage switching valve 21 on a flow of the mobile phase. In
other words, in place of fluid containing SFE-extracted components
using the extracting unit 114, the mobile phase containing the
sample is supplied to the splitting part 110. Then, after the
components are separated into individual components in the
analyzing column 109 similarly to the first embodiment, the mobile
phase flows in the first back pressure regulating valve 111 and the
mass spectrometer (MS) 113, where the individual components are
analyzed (SFC). Also in this embodiment, a detector (ultraviolet
detector (UV) 310 or the like) (not shown) may be provided between
the analyzing column 109 and the first back pressure regulating
valve 111, and the mass spectrometer 113 is not an essential
configuration.
[0047] In this way, in the analyzing device 20 of this embodiment,
a user can freely select and use the on-line configuration in which
SFE and SFC are integrated into one flow system or the
configuration in which only chromatography (SFC) using
supercritical fluid can be performed by changing the connections
between the ports in the third flow passage switching valve 21.
Therefore, a sample is not limited to one in the state before
performing SFE, which can widen the range of analysable samples
more than conventional and can enhance versatility of an analyzing
device.
[0048] The aforementioned embodiments are merely examples of the
present invention, and it is apparent that proper variations,
modification and additions within the spirit of the present
invention are included in the scope of the appended claims of the
present application. In any of the aforementioned embodiments,
while description has been made to the case where fluid containing
sample components flows separately in two flow passages of the
first flow passage and the second flow passage at the splitting
part 110, a configuration of an analyzing device according to the
present invention is not limited to this, but it may be configured
such that it flows separately in two or more plural flow passages
at the splitting part.
REFERENCE SIGNS LIST
[0049] 10, 20, 30 . . . Analyzing Device [0050] 21 . . . Third Flow
Passage Switching Valve [0051] 22 . . . Autosampler [0052] 100, 300
. . . Cylinder [0053] 101, 301 . . . Pressurizing Pump [0054] 102,
302 . . . Solvent Container [0055] 103, 303 . . . Modifier Pump
[0056] 104, 304 . . . First Flow Passage Switching Valve [0057]
105, 305 . . . Sample Storing Container [0058] 106, 306 . . .
Temperature Adjusting Device [0059] 107, 307 . . . Needle [0060]
108, 308 . . . Second Flow Passage Switching Valve [0061] 109, 309
. . . Analyzing Column [0062] 110 . . . Splitting Part [0063] 111 .
. . First Back Pressure Regulating Valve [0064] 112 . . . Second
Back Pressure Regulating Valve [0065] 113 . . . Mass Spectrometer
[0066] 114 . . . Extracting Unit [0067] 115 . . . Recovering
Container [0068] 310 . . . Ultraviolet Detector [0069] 311 . . .
Back Pressure Regulating Valve
* * * * *