U.S. patent application number 15/424709 was filed with the patent office on 2017-08-24 for methods for genomic modification. This patent application is currently assigned to Amyris, Inc.. The applicant listed for this patent is Amyris, Inc.. Invention is credited to Andrew Horwitz, Zach Serber.
Application Number | 20170240923 15/424709 |
Document ID | / |
Family ID | 46051948 |
Filed Date | 2017-08-24 |
United States Patent Application | 20170240923 |
Kind Code | A1 |
Serber; Zach ; et al. | August 24, 2017 |
Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, and a nuclease capable of causing a double-strand break near or within the genomic target site.
Inventors: | Serber; Zach; (Emeryville, CA) ; Horwitz; Andrew; (Emeryville, CA) | ||||||||||
Applicant: |
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Assignee: | Amyris, Inc. Emeryville CA |
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Family ID: | 46051948 | ||||||||||
Appl. No.: | 15/424709 | ||||||||||
Filed: | February 3, 2017 |
Application Number | Filing Date | Patent Number | ||
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14178203 | Feb 11, 2014 | 9701971 | ||
15424709 | ||||
13459034 | Apr 27, 2012 | 8685737 | ||
14178203 | ||||
61539389 | Sep 26, 2011 | |||
61500741 | Jun 24, 2011 | |||
61479821 | Apr 27, 2011 | |||
Current U.S. Class: | 1/1 |
Current CPC Class: | C12N 15/1093 20130101; C12N 15/905 20130101; C12N 15/81 20130101; C12N 15/1082 20130101 |
International Class: | C12N 15/90 20060101 C12N015/90; C12N 15/81 20060101 C12N015/81 |
Sequence CWU 1
1
661741DNAArtificial SequenceSynthetic Wild-type Em.GFP coding
sequence 1atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
cgagctggac 60ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
tgccacctac 120ggcaagctga ccctgaagtt catctgcacc accggcaagc
tgcccgtgcc ctggcccacc 180ctcgtgacca ccttgaccta cggcgtgcag
tgcttcgccc gctaccccga ccacatgaag 240cagcacgact tcttcaagtc
cgccatgccc gaaggctacg tccaggagcg caccatcttc 300ttcaaggacg
acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
360gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
cctggggcac 420aagctggagt acaactacaa cagccacaag gtctatatca
ccgccgacaa gcagaagaac 480ggcatcaagg tgaacttcaa gacccgccac
aacatcgagg acggcagcgt gcagctcgcc 540gaccactacc agcagaacac
ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600tacctgagca
cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
660ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
gtacaagtaa 720ctcgagaagc ttgatccggc t 741213DNAArtificial
SequenceSynthetic EmGFP linker encoding premature stop codon
2cgtctaaatc atg 133500DNAArtificial SequenceSynthetic HO upstream
integration sequence 3cgcaagtcct gtttctatgc ctttctctta gtaattcacg
aaataaacct atggtttacg 60aaatgatcca cgaaaatcat gttattattt acatcaacat
atcgcgaaaa ttcatgtcat 120gtccacatta acatcattgc agagcaacaa
ttcattttca tagagaaatt tgctactatc 180acccactagt actaccattg
gtacctacta ctttgaattg tactaccgct gggcgttatt 240aggtgtgaaa
ccacgaaaag ttcaccataa cttcgaataa agtcgcggaa aaaagtaaac
300agctattgct actcaaatga ggtttgcaga agcttgttga agcatgatga
agcgttctaa 360acgcactatt catcattaaa tatttaaagc tcataaaatt
gtattcaatt cctattctaa 420atggctttta tttctattac aactattagc
tctaaatcca tatcctcata agcagcaatc 480aattctatct atactttaaa
5004200DNAArtificial SequenceSynthetic HO downstream integration
sequence 4aatgtgtata ttagtttaaa aagttgtatg taataaaagt aaaatttaat
attttggatg 60aaaaaaacca tttttagact ttttcttaac tagaatgctg gagtagaaat
acgccatctc 120aagatacaaa aagcgttacc ggcactgatt tgtttcaacc
agtatataga ttattattgg 180gtcttgatca actttcctca 2005490DNAArtificial
SequenceSynthetic YGR250c upstream integration sequence 5gtacgatgtt
tctcccgctg atccgattac tagccgaaga cgtaaaattg gcgcttgatt 60caatttatgc
ccttcccggg aatagttgac caaagggcaa aaaaattcag tcggagattc
120cctattgggc ggaatttagt agatctcttt ccgtgcataa cgcctgcccg
ttagtcgtta 180tttcacgtta acattttctt ggccactgcg ctatataaat
aaatacatat atatatgtca 240agcacaataa agaaacttcc cttaaatatt
gaataagtaa ataatagttg aaaagtgcct 300tttgttcgaa ggattagagt
gttcttaatt ttagttcgtt caacggtctc aaaaaaagtg 360tgaacaagta
aagcatagca cacatcccaa attacaaggc accctgatta aaaatccaaa
420aataaaccat aagttttatt ttactaaaaa cattatacgt gaaagacaaa
ccgcatcaga 480agtttcgagg 4906185DNAArtificial SequenceSynthetic
YGR250c downstream integration sequence 6attgcatcag gtccataaaa
tgtttttgtc tgcttttttt tcttcatgta ttagttggtt 60tttattttta tattttcatt
tatcttattc atacttttta ctcctttttt cttcattctt 120tacgatcttg
gacattcaac tagcctatgg taacttttct tattactttg cccctccttg 180aggtg
1857504DNAArtificial SequenceSynthetic NDT80 upstream integration
sequence 7catcaagcgc tccaagctga cataaatcgc actttgtatc tacttttttt
tattcgaaaa 60caaggcacaa caatgaatct atcgccctgt gagattttca atctcaagtt
tgtgtaatag 120atagcgttat attatagaac tataaaggtc cttgaatata
catagtgttt cattcctatt 180actgtatatg tgactttaca ttgttacttc
cgcggctatt tgacgttttc tgcttcaggt 240gcggcttgga gggcaaagtg
tcagaaaatc ggccaggccg tatgacacaa aagagtagaa 300aacgagatct
caaatatctc gaggcctgtc ctctatacaa ccgcccagct ctctgacaaa
360gctccagaac ggttgtcttt tgtttcgaaa agccaaggtc ccttataatt
gccctccatt 420ttgtgtcacc tatttaagca aaaaattgaa agtttactaa
cctttcatta aagagaaata 480acaatattat aaaaagcgct taaa
5048202DNAArtificial SequenceSynthetic NDT80 downstream integration
sequence 8ataaactaat gattttaaat cgttaaaaaa atatgcgaat tctgtggatc
gaacacagga 60cctccagata acttgaccga agttttttct tcagtctggc gctctcccaa
ctgagctaaa 120tccgcttact atttgttatc agttcccttc atatctacat
agaataggtt aagtatttta 180ttagttgcca gaagaactac tg
202943DNAArtificial SequenceSynthetic Recognition sequence for
ZFN.gfp 9acaactacaa cagccacaac gtctatatca tggccgacaa gca
431031DNAArtificial SequenceSynthetic Forward primer specific to
Em.GFP 10caactacaac agccacaagg tctatatcac c 311122DNAArtificial
SequenceSynthetic Reverse primer for HO locus 11ctctaacgct
gttggtagat tg 221239DNAArtificial SequenceSynthetic Reverse primer
for NDT80 locus 12accatgtgat aatacactac taatgtgact actagttga
391330DNAArtificial SequenceSynthetic Reverse primer for YGR250c
locus 13tcagacgcgt tcggaggaga gtgcattcac 30145251DNAArtificial
SequenceSynthetic Integration construct i8 14ttgcctatgc tttgtttgct
ttgaacactt gtttccgctc tccttttact tattggctac 60taaaactacg tgtaaaagat
cgcccagcgc aaaaaggtcc ggcggtttca aataatctcg 120aactattcct
ataatatgca aaatagtagg taggaacaag tcgactctag gcagataagg
180aagatgtccg gtaaatggag actagtgctg accgggatag gcaatccaga
gcctcagtac 240gctggtaccc gtcacaatgt agggctatat atgctggagc
tgctacgaaa gcggcttggt 300ctgcagggga gaacttattc ccctgtgcct
aatacgggcg gcaaagtgca ttatatagaa 360gacgaacatt gtacgatact
aagatcggat ggccagtaca tgaatctaag tggagaacag 420gtgtgcaagg
tctgggcccg gtacgccaag taccaagccc gacacgtagt tattcatgac
480gagttaagtg tggcgtgtgg aaaagtgcag ctcagagccc ccagcaccag
tattagaggt 540cataatgggc tgcgaagcct gctaaaatgc agtggaggcc
gtgtaccctt tgccaaattg 600gctattggaa tcggcagaga acctgggtcc
cgttctagag accctgcgag cgtgtcccgg 660tgggttctgg gagctctaac
tccgcaggaa ctacaaacct tgcttacaca gagtgaacct 720gctgcctggc
gtgctctgac tcagtacatt tcatagtgga tggcggcgtt agtatcgaat
780cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta
ctttctgcgc 840acttaacttc gcatctgggc agatgatgtc gaggcgaaaa
aaaatataaa tcacgctaac 900atttgattaa aatagaacaa ctacaatata
aaaaaactat acaaatgaca agttcttgaa 960aacaagaatc tttttattgt
cagtactgat tagaaaaact catcgagcat caaatgaaac 1020tgcaatttat
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
1080gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
tcggtctgcg 1140attccgactc gtccaacatc aatacaacct attaatttcc
cctcgtcaaa aataaggtta 1200tcaagtgaga aatcaccatg agtgacgact
gaatccggtg agaatggcaa aagcttatgc 1260atttctttcc agacttgttc
aacaggccag ccattacgct cgtcatcaaa atcactcgca 1320tcaaccaaac
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
1380ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
tgccagcgca 1440tcaacaatat tttcacctga atcaggatat tcttctaata
cctggaatgc tgttttgccg 1500gggatcgcag tggtgagtaa ccatgcatca
tcaggagtac ggataaaatg cttgatggtc 1560ggaagaggca taaattccgt
cagccagttt agtctgacca tctcatctgt aacatcattg 1620gcaacgctac
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
1680cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
cccatataaa 1740tcagcatcca tgttggaatt taatcgcggc ctcgaaacgt
gagtcttttc cttacccatg 1800gttgtttatg ttcggatgtg atgtgagaac
tgtatcctag caagatttta aaaggaagta 1860tatgaaagaa gaacctcagt
ggcaaatcct aaccttttat atttctctac aggggcgcgg 1920cgtggggaca
attcaacgcg tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg
1980aggagccgta atttttgctt cgcgccgtgc ggccatcaaa atgtatggat
gcaaatgatt 2040atacatgggg atgtatgggc taaatgtacg ggcgacagtc
acatcatgcc cctgagctgc 2100gcacgtcaag actgtcaagg agggtattct
gggcctccat gtcgctggcc gggtgacccg 2160gcggggacga ggcaagctaa
acagatctga tcttgaaact gagtaagatg ctcagaatac 2220ccgtcaagat
aagagtataa tgtagagtaa tataccaagt attcagcata ttctcctctt
2280cttttgtata aatcacggaa gggatgattt ataagaaaaa tgaatactat
tacacttcat 2340ttaccaccct ctgatctaga ttttccaacg atatgtacgt
agtggtataa ggtgaggggg 2400tccacagata taacatcgtt taatttagta
ctaacagaga cttttgtcac aactacatat 2460aagtgtacaa atatagtaca
gatatgacac acttgtagcg ccaacgcgca tcctacggat 2520tgctgacaga
aaaaaaggtc acgtgaccag aaaagtcacg tgtaattttg taactcaccg
2580cattctagcg gtccctgtcg tgcacactgc actcaacacc ataaacctta
gcaacctcca 2640aaggaaatca ccgtataaca aagccacagt tttacaactt
agtctcttat gaagttactt 2700accaatgaga aatagaggct ctttctcgag
aaatatgaat atggatatat atatatatat 2760atatatatat atatatatat
gtaaacttgg ttctttttta gcttgtgatc tctagcttgg 2820gtctctctct
gtcgtaacag ttgtgatatc ggaagaagag aaaagacgaa gagcagaagc
2880ggaaaacgta tacacgtcac atatcacaca cacacaatgg gaaagctatt
acaattggca 2940ttgcatccgg tcgagatgaa ggcagctttg aagctgaagt
tttgcagaac accgctattc 3000tccatctatg atcagtccac gtctccatat
ctcttgcact gtttcgaact gttgaacttg 3060acctccagat cgtttgctgc
tgtgatcaga gagctgcatc cagaattgag aaactgtgtt 3120actctctttt
atttgatttt aagggctttg gataccatcg aagacgatat gtccatcgaa
3180cacgatttga aaattgactt gttgcgtcac ttccacgaga aattgttgtt
aactaaatgg 3240agtttcgacg gaaatgcccc cgatgtgaag gacagagccg
ttttgacaga tttcgaatcg 3300attcttattg aattccacaa attgaaacca
gaatatcaag aagtcatcaa ggagatcacc 3360gagaaaatgg gtaatggtat
ggccgactac atcttagatg aaaattacaa cttgaatggg 3420ttgcaaaccg
tccacgacta cgacgtgtac tgtcactacg tagctggttt ggtcggtgat
3480ggtttgaccc gtttgattgt cattgccaag tttgccaacg aatctttgta
ttctaatgag 3540caattgtatg aaagcatggg tcttttccta caaaaaacca
acatcatcag agattacaat 3600gaagatttgg tcgatggtag atccttctgg
cccaaggaaa tctggtcaca atacgctcct 3660cagttgaagg acttcatgaa
acctgaaaac gaacaactgg ggttggactg tataaaccac 3720ctcgtcttaa
acgcattgag tcatgttatc gatgtgttga cttatttggc cggtatccac
3780gagcaatcca ctttccaatt ttgtgccatt ccccaagtta tggccattgc
aaccttggct 3840ttggtattca acaaccgtga agtgctacat ggcaatgtaa
agattcgtaa gggtactacc 3900tgctatttaa ttttgaaatc aaggactttg
cgtggctgtg tcgagatttt tgactattac 3960ttacgtgata tcaaatctaa
attggctgtg caagatccaa atttcttaaa attgaacatt 4020caaatctcca
agatcgaaca gtttatggaa gaaatgtacc aggataaatt acctcctaac
4080gtgaagccaa atgaaactcc aattttcttg aaagttaaag aaagatccag
atacgatgat 4140gaattggttc caacccaaca agaagaagag tacaagttca
atatggtttt atctatcatc 4200ttgtccgttc ttcttgggtt ttattatata
tacactttac acagagcgtg aagtctgcgc 4260caaataacat aaacaaacaa
ctccgaacaa taactaagta cttacataat aggtagaggc 4320ctatccttaa
agataacctt atatttcatt acatcaacta attcgacctt attatctttc
4380gaattgaaat gcattatacc catcggtacg tctagctttg tcaccttccc
cagtaaacgc 4440tgtttcttgc cgacaaacaa tgtggccctc tctccgtcaa
tctgtaacga cccaaatcgt 4500attaaagttt cgccgtcctg ttcactgaac
cttccctcat ttggagaatc tctcctcgcc 4560agcgacgcaa agtccttagg
caactctagt tcaccttgaa tctccagcat catcatccca 4620agcggtgtta
tcaccgtggt ctgcttttct cttgactgtg tcaacttctg ccattgacta
4680gcatctatat ctacactagg cattcttttc agctgtttat tgggctgaat
gatagtgata 4740attctttttt ctatcactcc tttggctata ttagtggtta
gcttactaaa aaagattaaa 4800ggaaaaatga aattcaagat gctaacgttg
acatgtatat tttaagaaaa caaaaatcat 4860acaaagagga gatcggatat
aaaagaataa cataaatatg tttagtgcat taggtaaatg 4920ggtccgaggc
tctcgcaatg ataaggactt tgtgacgaag tataccgcag atttatcaca
4980aataacttca cagatccatc aattagatgt cgcgttaaag aaaagccaat
ccatcttgag 5040tcaatggcaa tcaaatctga ccttttatgg tattgcgtta
acggtattgg ccctgagcta 5100cacatattgg gagtaccatg gttatcgacc
ataccttgtg gtgactgcgc tactatgcat 5160aggctcgcta atcttgttca
aatgggcatt aaccaaactc tatgcatttt ataacaacaa 5220taggttacgc
aagttggcaa aactccgtgc a 52511570DNAArtificial SequenceSynthetic
Primer 61-67-CPK066-G 15ggtaagacgg ttgggtttta tcttttgcag ttggtactat
taagaacaat cacaggaaac 60agctatgacc 701670DNAArtificial
SequenceSynthetic Primer 61-67-CPK067-G 16ttgcgttttg tactttggtt
cgctcaattt tgcaggtaga taatcgaaaa gttgtaaaac 60gacggccagt
70174162DNAArtificial SequenceSynthetic Integration construct i32
17gcctgtctac aggataaaga cgggtcggat acctgcacaa gcaatttggc acctgcatac
60cccatttccc cagtagataa cttcaacaca cacatcaatg tccctcacca gtttatttcc
120aaaagagacg ctttttacta cctgactaga ttttcatttt gtttcttttg
gattgcgctt 180gcctttgtag gtgtgtcgtt tatcctttac gttttgactt
ggtgctcgaa gatgctttca 240gagatggtgc ttatcctcat gtcttttggg
tttgtcttca atacggcagc cgttgtcttg 300caaacggccg cctctgccat
ggcaaagaat gctttccatg acgatcatcg tagtgcccaa 360ttgggtgcct
ctatgatggg tatggcttgg gcaagtgtct ttttatgtat cgtggaattt
420atcctgctgg tcttctggtc tgttagggca aggttggcct ctacttactc
catcgacaat 480tcaagataca gaacctcctc cagatggaat cccttccata
gagagaagga gcaagcaact 540gacccaatat tgactgccac tggacctgaa
gacatgcaac aaagtgcaag catagtgggg 600ccttcttcca atgctaatcc
ggtcactgcc actgctgcta cggaaaacca acctaaaggt 660attaacttct
tcactataag aaaatcacac gagcgcccgg acgatgtctc tgtttaaatg
720gcgcaagttt tccgctttgt aatatatatt tatacccctt tcttctctcc
cctgcaatat 780aatagtttaa ttctaatatt aataatatcc tatattttct
tcatttaccg gcgcactctc 840gcccgaacga cctcaaaatg tctgctacat
tcataataac caaaagctca taactttttt 900ttttgaacct gaatatatat
acatcacata tcactgctgg tccttgccga ccagcgtata 960caatctcgat
agttggtttc ccgttctttc cactcccgtc cacaggaaac agctatgacc
1020atgattacgc caagctattt aggtgacact atagaatact caagctatgc
atcaagcttg 1080gtaccgagct cggatccact agtaacggcc gccagtgtgc
tggaattcgc cctgtcgaca 1140ctagtaatac acatcatcgt cctacaagtt
catcaaagtg ttggacagac aactatacca 1200gcatggatct cttgtatcgg
ttcttttctc ccgctctctc gcaataacaa tgaacactgg 1260gtcaatcata
gcctacacag gtgaacagag tagcgtttat acagggttta tacggtgatt
1320cctacggcaa aaatttttca tttctaaaaa aaaaaagaaa aatttttctt
tccaacgcta 1380gaaggaaaag aaaaatctaa ttaaattgat ttggtgattt
tctgagagtt ccctttttca 1440tatatcgaat tttgaatata aaaggagatc
gaaaaaattt ttctattcaa tctgttttct 1500ggttttattt gatagttttt
ttgtgtatta ttattatgga ttagtactgg tttatatggg 1560tttttctgta
taacttcttt ttattttagt ttgtttaatc ttattttgag ttacattata
1620gttccctaac tgcaagagaa gtaacattaa aaatgaaaaa gcctgaactc
accgcgacgt 1680ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
cgacctgatg cagctctcgg 1740agggcgaaga atctcgtgct ttcagcttcg
atgtaggagg gcgtggatat gtcctgcggg 1800taaatagctg cgccgatggt
ttctacaaag atcgttatgt ttatcggcac tttgcatcgg 1860ccgcgctccc
gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
1920gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
gaactgcccg 1980ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
tgcggccgat cttagccaga 2040cgagcgggtt cggcccattc ggaccgcaag
gaatcggtca atacactaca tggcgtgatt 2100tcatatgcgc gattgctgat
ccccatgtgt atcactggca aactgtgatg gacgacaccg 2160tcagtgcgtc
cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
2220aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
gacaatggcc 2280gcataacagc ggtcattgac tggagcgagg cgatgttcgg
ggattcccaa tacgaggtcg 2340ccaacatctt cttctggagg ccgtggttgg
cttgtatgga gcagcagacg cgctacttcg 2400agcggaggca tccggagctt
gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg 2460gtcttgacca
actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
2520agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
acacaaatcg 2580cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
agtactcgcc gatagtggaa 2640accgacgccc cagcactcgt ccgagggcaa
aggaataggt ttaacttgat actactagat 2700tttttctctt catttataaa
atttttggtt ataattgaag ctttagaagt atgaaaaaat 2760cctttttttt
cattctttgc aaccaaaata agaagcttct tttattcatt gaaatgatga
2820atataaacct aacaaaagaa aaagactcga atatcaaaca ttaaaaaaaa
ataaaagagg 2880ttatctgttt tcccatttag ttggagtttg cattttctaa
tagatagaac tctcaattaa 2940tgtggattta gtttctctgt tcgttttttt
ttgttttgtt ctcactgtat ttacatttct 3000atttagtatt tagttattca
tataatctta acttctcgag gagctctaag ggcgaattct 3060gcagatatcc
atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct
3120atagtgagtc gtattacaat tcactggccg tcgttttaca acaagcatct
tgccctgtgc 3180ttggccccca gtgcagcgaa cgttataaaa acgaatactg
agtatatatc tatgtaaaac 3240aaccatatca tttcttgttc tgaactttgt
ttacctaact agttttaaat ttcccttttt 3300cgtgcatgcg ggtgttctta
tttattagca tactacattt gaaatatcaa atttccttag 3360tagaaaagtg
agagaaggtg cactgacaca aaaaataaaa tgctacgtat aactgtcaaa
3420actttgcagc agcgggcatc cttccatcat agcttcaaac atattagcgt
tcctgatctt 3480catacccgtg ctcaaaatga tcaaacaaac tgttattgcc
aagaaataaa cgcaaggctg 3540ccttcaaaaa ctgatccatt agatcctcat
atcaagcttc ctcatagaac gcccaattac 3600aataagcatg ttttgctgtt
atcaccgggt gataggtttg ctcaaccatg gaaggtagca 3660tggaatcata
atttggatac taatacaaat cggccatata atgccattag taaattgcgc
3720tcccatttag gtggttctcc aggaatacta ataaatgcgg tgcatttgca
aaatgaattt 3780attccaaggc caaaacaaca cgatgaatgg ctttattttt
ttgttattcc tgacatgaag 3840ctttatgtaa ttaaggaaac ggacatcgag
gaatttgcat cttttttaga tgaaggagct 3900attcaagcac caaagctatc
cttccaggat tatttaagcg gtaaggccaa ggcttcccaa 3960caggttcatg
aagtgcatca tagaaagctt acaaggtttc agggtgaaac ttttctaaga
4020gattggaact tagtctgtgg gcattataag agagatgcta agtgtggaga
aatgggaccc 4080gacataattg cagcatttca agatgaaaag ctttttcctg
agaataatct agccttaatt 4140tctcatattg ggggtcatat tt
4162187879DNAArtificial SequenceSynthetic Integration construct
i33misc_feature(270)..(270)n is a, c, g, or t 18atgaattggc
cagttttttc caattatgga acgcctgttc ctgatccacg gcctgcactt 60gcgaccacaa
ttccacacct gaggcgcctg cctcttttcc agcatgtggc aactgtcccc
120acgacagggc atcccagaat cctctggtaa atcttaaatg aaactgacgc
gtggcagtag 180attccaacaa tggtgggatg gcccgtggga aagtcgtgta
gtgctcatac gcatcatatg 240acatggatga tacggccggg tcaaacggtn
cgattgcagt tggaatgcaa atgagagtag 300cagatcattg ttgggcagcg
gcttcaacac cagtgcttcg tcgtacggat accataaact 360gtcatttata
ccaatctgcg acaccgtgtc ttctgcgaac acacccagca gtagagtgcc
420cagcatgaaa taggccagtg tgaggatcat cgtcgtcttg cctatgcttt
gtttgctttg 480aacacttgtt tccgctctcc ttttacttat tggctactaa
aactacgtgt aaaagatcgc 540ccagcgcaaa aaggtccggc ggtttcaaat
aatctcgaac tattcctata atatgcaaaa 600tagtaggtag gaacaagtca
actctaggca gataacgaag atgtccggta aatggagact 660agtgctgact
gggataggca
atccagagcc tcagtacgct ggcacccgtc acaatgtagg 720gctatatatg
ctggagctgc tacgaaagcg gcttggtctg caggggagaa cttattcccc
780tgtgcctaat acgggcggca aagtgcatta tatagaagac gaacattgta
cgatactaag 840atcggatggc cagtacatga atctaagtgg agaacaggtg
tgcaaggtct gggcccggta 900cgccaagtac caagcccgac acgttgttat
tcatgacgag ttaagtgtgg cgtgtggaaa 960agtgcagctc agagccccca
gcaccagtat tagaggtcat aatgggctgc gaagcctgct 1020aaaatgcagt
ggaggccgtg taccctttgc caaattggct attggaatcg gcagagaacc
1080tgggtcccgt tctagagacc ctgcgagcgt gtcccggtgg gttctgggag
ctctaactcc 1140gcaggaacta caaaccttgc ttacacagag tgaacctgct
gcctggcgtg ctctgactca 1200gtacatttca taggacagca ttcgcccagt
atttttttta ttctacaaac cttctataat 1260ttcaaagtat ttacataatt
ctgtatcagt ttaatcacca taatatcgtt ttctttgttt 1320agtgcaatta
atttttccta ttgttacttc gggccttttt ctgttttatg agctattttt
1380tccgtcatcc ttccggatcc agattttcag cttcatctcc agattgtgtc
tacgtaatgc 1440acgccatcat tttaagagag gacagagaag caagcctcct
gaaagatgaa gctactgtct 1500tctatcgaac aagcatgcga tatttgccga
cttaaaaagc tcaagtgctc caaagaaaaa 1560ccgaagtgcg ccaagtgtct
gaagaacaac tgggagtgtc gctactctcc caaaaccaaa 1620aggtctccgc
tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
1680cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
aatggattct 1740ttacaggata taaaagcatt gttaacagga ttatttgtac
aagataatgt gaataaagat 1800gccgtcacag atagattggc ttcagtggag
actgatatgc ctctaacatt gagacagcat 1860agaataagtg cgacatcatc
atcggaagag agtagtaaca aaggtcaaag acagttgact 1920gtatcgattg
actcggcagc tcatcatgat aactccacaa ttccgttgga ttttatgccc
1980agggatgctc ttcatggatt tgattggtct gaagaggatg acatgtcgga
tggcttgccc 2040ttcctgaaaa cggaccccaa caataatggg ttctttggcg
acggttctct cttatgtatt 2100cttcgatcta ttggctttaa accggaaaat
tacacgaact ctaacgttaa caggctcccg 2160accatgatta cggatagata
cacgttggct tctagatcca caacatcccg tttacttcaa 2220agttatctca
ataattttca cccctactgc cctatcgtgc actcaccgac gctaatgatg
2280ttgtataata accagattga aatcgcgtcg aaggatcaat ggcaaatcct
ttttaactgc 2340atattagcca ttggagcctg gtgtatagag ggggaatcta
ctgatataga tgttttttac 2400tatcaaaatg ctaaatctca tttgacgagc
aaggtcttcg agtcaggttc cataattttg 2460gtgacagccc tacatcttct
gtcgcgatat acacagtgga ggcagaaaac aaatactagc 2520tataattttc
acagcttttc cataagaatg gccatatcat tgggcttgaa tagggacctc
2580ccctcgtcct tcagtgatag cagcattctg gaacaaagac gccgaatttg
gtggtctgtc 2640tactcttggg agatccaatt gtccctgctt tatggtcgat
ccatccagct ttctcagaat 2700acaatctcct tcccttcttc tgtcgacgat
gtgcagcgta ccacaacagg tcccaccata 2760tatcatggca tcattgaaac
agcaaggctc ttacaagttt tcacaaaaat ctatgaacta 2820gacaaaacag
taactgcaga aaaaagtcct atatgtgcaa aaaaatgctt gatgatttgt
2880aatgagattg aggaggtttc gagacaggca ccaaagtttt tacaaatgga
tatttccacc 2940accgctctaa ccaatttgtt gaaggaacac ccttggctat
cctttacaag attcgaactg 3000aagtggaaac agttgtctct tatcatttat
gtattaagag attttttcac taattttacc 3060cagaaaaagt cacaactaga
acaggatcaa aatgatcatc aaagttatga agttaaacga 3120tgctccatca
tgttaagcga tgcagcacaa agaactgtta tgtctgtaag tagctatatg
3180gacaatcata atgtcacccc atattttgcc tggaattgtt cttattactt
gttcaatgca 3240gtcctagtac ccataaagac tctactctca aactcaaaat
cgaatgctga gaataacgag 3300accgcacaat tattacaaca aattaacact
gttctgatgc tattaaaaaa actggccact 3360tttaaaatcc agacttgtga
aaaatacatt caagtactgg aagaggtatg tgcgccgttt 3420ctgttatcac
agtgtgcaat cccattaccg catatcagtt ataacaatag taatggtagc
3480gccattaaaa atattgtcgg ttctgcaact atcgcccaat accctactct
tccggaggaa 3540aatgtcaaca atatcagtgt taaatatgtt tctcctggct
cagtagggcc ttcacctgtg 3600ccattgaaat caggagcaag tttcagtgat
ctagtcaagc tgttatctaa ccgtccaccc 3660tctcgtaact ctccagtgac
aataccaaga agcacacctt cgcatcgctc agtcacgcct 3720tttctagggc
aacagcaaca gctgcaatca ttagtgccac tgaccccgtc tgctttgttt
3780ggtggcgcca attttaatca aagtgggaat attgctgata gctcattgtc
cttcactttc 3840actaacagta gcaacggtcc gaacctcata acaactcaaa
caaattctca agcgctttca 3900caaccaattg cctcctctaa cgttcatgat
aacttcatga ataatgaaat cacggctagt 3960aaaattgatg atggtaataa
ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg 4020tataacgcgt
ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat
4080aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagta
aaatgaatcg 4140tagatactga aaaaccccgc aagttcactt caactgtgca
tcgtgcacca tctcaatttc 4200tttcatttat acatcgtttt gccttctttt
atgtaactat actcctctaa gtttcaatct 4260tggccatgta acctctgatc
tatagaattt tttaaatgac tagaattaat gcccatcttt 4320tttttggacc
taaattcttc atgaaaatat attacgaggg cttattcaga agcttcgctc
4380agtcgacact agtaatacac atcatcgtcc tacaagttca tcaaagtgtt
ggacagacaa 4440ctataccagc atggatctct tgtatcggtt cttttctccc
gctctctcgc aataacaatg 4500aacactgggt caatcatagc ctacacaggt
gaacagagta gcgtttatac agggtttata 4560cggtgattcc tacggcaaaa
atttttcatt tctaaaaaaa aaaagaaaaa tttttctttc 4620caacgctaga
aggaaaagaa aaatctaatt aaattgattt ggtgattttc tgagagttcc
4680ctttttcata tatcgaattt tgaatataaa aggagatcga aaaaattttt
ctattcaatc 4740tgttttctgg ttttatttga tagttttttt gtgtattatt
attatggatt agtactggtt 4800tatatgggtt tttctgtata acttcttttt
attttagttt gtttaatctt attttgagtt 4860acattatagt tccctaactg
caagagaagt aacattaaaa atgaccactc ttgacgacac 4920ggcttaccgg
taccgcacca gtgtcccggg ggacgccgag gccatcgagg cactggatgg
4980gtccttcacc accgacaccg tcttccgcgt caccgccacc ggggacggct
tcaccctgcg 5040ggaggtgccg gtggacccgc ccctgaccaa ggtgttcccc
gacgacgaat cggacgacga 5100atcggacgcc ggggaggacg gcgacccgga
ctcccggacg ttcgtcgcgt acggggacga 5160cggcgacctg gcgggcttcg
tggtcgtctc gtactccggc tggaaccgcc ggctgaccgt 5220cgaggacatc
gaggtcgccc cggagcaccg ggggcacggg gtcgggcgcg cgttgatggg
5280gctcgcgacg gagttcgccc gcgagcgggg cgccgggcac ctctggctgg
aggtcaccaa 5340cgtcaacgca ccggcgatcc acgcgtaccg gcggatgggg
ttcaccctct gcggcctgga 5400caccgccctg tacgacggca ccgcctcgga
cggcgagcag gcgctctaca tgagcatgcc 5460ctgcccctga gtttaacttg
atactactag attttttctc ttcatttata aaatttttgg 5520ttataattga
agctttagaa gtatgaaaaa atcctttttt ttcattcttt gcaaccaaaa
5580taagaagctt cttttattca ttgaaatgat gaatataaac ctaacaaaag
aaaaagactc 5640gaatatcaaa cattaaaaaa aaataaaaga ggttatctgt
tttcccattt agttggagtt 5700tgcattttct aatagataga actctcaatt
aatgtggatt tagtttctct gttcgttttt 5760ttttgttttg ttctcactgt
atttacattt ctatttagta tttagttatt catataatct 5820taacttctcg
aggagctcga tcttgaaact gagtaagatg ctcagaatac ccgtcaagat
5880aagagtataa tgtagagtaa tataccaagt attcagcata ttctcctctt
cttttgtata 5940aatcacggaa gggatgattt ataagaaaaa tgaatactat
tacacttcat ttaccaccct 6000ctgatctaga ttttccaacg atatgtacgt
agtggtataa ggtgaggggg tccacagata 6060taacatcgtt taatttagta
ctaacagaga cttttgtcac aactacatat aagtgtacaa 6120atatagtaca
gatatgacac acttgtagcg ccaacgcgca tcctacggat tgctgacaga
6180aaaaaaggtc acgtgaccag aaaagtcacg tgtaattttg taactcaccg
cattctagcg 6240gtccctgtcg tgcacactgc actcaacacc ataaacctta
gcaacctcca aaggaaatca 6300ccgtataaca aagccacagt tttacaactt
agtctcttat gaagtgtctc tctctgtcgt 6360aacagttgtg atatcggaag
aagagaaaag acgaagagca gaagcggaaa acgtatacac 6420gtcacatatc
acacacacac aatgggaaag ctattacaat tggcattgca tccggtcgag
6480atgaaggcag ctttgaagct gaagttttgc agaacaccgc tattctccat
ctatgatcag 6540tccacgtctc catatctctt gcactgtttc gaactgttga
acttgacctc cagatcgttt 6600gctgctgtga tcagagagct gcatccagaa
ttgagaaact gtgttactct cttttatttg 6660attttaaggg ctttggatac
catcgaagac gatatgtcca tcgaacacga tttgaaaatt 6720gacttgttgc
gtcacttcca cgagaaattg ttgttaacta aatggagttt cgacggaaat
6780gcccccgatg tgaaggacag agccgttttg acagatttcg aatcgattct
tattgaattc 6840cacaaattga aaccagaata tcaagaagtc atcaaggaga
tcaccgagaa aatgggtaat 6900ggtatggccg actacatctt ggatgaaaat
tacaacttga atgggttgca aaccgtccac 6960gactacgacg tgtactgtca
ctacgtagct ggtttggtcg gtgatggttt gacccgtttg 7020attgtcattg
ccaagtttgc caacgaatct ttgtattcta atgagcaatt gtatgaaagc
7080atgggtcttt tcctacaaaa aaccaacatc atcagagact acaatgaaga
tttggtcgat 7140ggtagatcct tctggcccaa ggaaatctgg tcacaatacg
ctcctcagtt gaaggacttc 7200atgaaacctg aaaacgaaca actggggttg
gactgtataa accacctcgt cttaaacgca 7260ttgagtcatg ttatcgatgt
gttgacttat ttggccagta tccacgagca atccactttc 7320caattttgtg
ccattcccca agttatggcc attgcaacct tggctttggt attcaacaac
7380cgtgaagtgc tacatggcaa tgtaaagatt cgtaagggta ctacctgcta
tttaattttg 7440aaatcaagga ctttgcgtgg ctgtgtcgag atttttgact
attacttacg tgatatcaaa 7500tctaaattgg ctgtgcaaga tccaaatttc
ttaaaattga acattcaaat ctccaagatc 7560gaacaattca tggaagaaat
gtaccaggat aaattacctc ctaacgtgaa gccaaatgaa 7620actccaattt
tcttgaaagt taaagaaaga tccagatacg atgatgaatt ggtcccaacc
7680caacaagaag aagagtacaa gttcaatatg gttttatcta tcatcttgtc
cgttcttctt 7740gggttttatt atatatacac tttacacaga gcgtgaagtc
tgcgccaaat aacataaaca 7800aacaactccg aacaataact aagtacttac
ataataggta gaggcctatc cttaaagata 7860accttatatt tcattacat
7879195714DNAArtificial SequenceSynthetic Integration construct i37
19gcctgtctac aggataaaga cgggtcggat acctgcacaa gcaatttggc acctgcatac
60cccatttccc cagtagataa cttcaacaca cacatcaatg tccctcacca gtttatttcc
120aaaagagacg ctttttacta cctgactaga ttttcatttt gtttcttttg
gattgcgctt 180gcctttgtag gtgtgtcgtt tatcctttac gttttgactt
ggtgctcgaa gatgctttca 240gagatggtgc ttatcctcat gtcttttggg
tttgtcttca atacggcagc cgttgtcttg 300caaacggccg cctctgccat
ggcaaagaat gctttccatg acgatcatcg tagtgcccaa 360ttgggtgcct
ctatgatggg tttaaacgta tggcttgggc aagtgtcttt ttatgtatcg
420tggaatttat cctgctggtc ttctggtctg ttagggcaag gttggcctct
acttactcca 480tcgacaattc aagatacaga acctcctcca gatggaatcc
cttccataga gagaaggagc 540aagcaactga cccaatattg actgccactg
gacctgaaga catgcaacaa agtgcaagca 600tagtggggcc ttcttccaat
gctaatccgg tcactgccac tgctgctacg gaaaaccaac 660ctaaaggtat
taacttcttc actataagaa aatcacacga gcgcccggac gatgtctctg
720tttaaatggc gcaagttttc cgctttgtaa tatatattta tacccctttc
ttctctcccc 780tgcaatataa tagtttaatt ctaatattaa taatatccta
tattttcttc atttaccggc 840gcactctcgc ccgaacgacc tcaaaatgtc
tgctacattc ataataacca aaagctcata 900actttttttt ttgaacctga
atatatatac atcacatatc actgctggtc ctgagaagtt 960aagattatat
gaataactaa atactaaata gaaatgtaaa tacagtgaga acaaaacaaa
1020aaaaaacgaa cagagaaact aaatccacat taattgagag ttctatctat
tagaaaatgc 1080aaactccaac taaatgggaa aacagataac ctcttttatt
tttttttaat gtttgatatt 1140cgagtctttt tcttttgtta ggtttatatt
catcatttca atgaataaaa gaagcttctt 1200attttggttg caaagaatga
aaaaaaagga ttttttcata cttctaaagc ttcaattata 1260accaaaaatt
ttataaatga agagaaaaaa tctagtagta tcaagttaaa cttagaaaaa
1320ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
taccatattt 1380ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
aggcagttcc ataggatggc 1440aagatcctgg tatcggtctg cgattccgac
tcgtccaaca tcaatacaac ctattaattt 1500cccctcgtca aaaataaggt
tatcaagtga gaaatcacca tgagtgacga ctgaatccgg 1560tgagaatggc
aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
1620ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
gcgcctgagc 1680gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
acaggaatcg aatgcaaccg 1740gcgcaggaac actgccagcg catcaacaat
attttcacct gaatcaggat attcttctaa 1800tacctggaat gctgttttgc
cggggatcgc agtggtgagt aaccatgcat catcaggagt 1860acggataaaa
tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
1920catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
acaactctgg 1980cgcatcgggc ttcccataca atcgatagat tgtcgcacct
gattgcccga cattatcgcg 2040agcccattta tacccatata aatcagcatc
catgttggaa tttaatcgcg gcctcgaaac 2100gtgagtcttt tccttaccca
tttttaatgt tacttctctt gcagttaggg aactataatg 2160taactcaaaa
taagattaaa caaactaaaa taaaaagaag ttatacagaa aaacccatat
2220aaaccagtac taatccataa taataataca caaaaaaact atcaaataaa
accagaaaac 2280agattgaata gaaaaatttt ttcgatctcc ttttatattc
aaaattcgat atatgaaaaa 2340gggaactctc agaaaatcac caaatcaatt
taattagatt tttcttttcc ttctagcgtt 2400ggaaagaaaa atttttcttt
ttttttttag aaatgaaaaa tttttgccgt aggaatcacc 2460gtataaaccc
tgtataaacg ctactctgtt cacctgtgta ggctatgatt gacccagtgt
2520tcattgttat tgcgagagag cgggagaaaa gaaccgatac aagagatcca
tgctggtata 2580gttgtctgtc caacactttg atgaacttgt aggacgatga
tgtgtattac tagtgtcgac 2640accatataca tatccatatc taatcttact
tatatgttgt ggaaatgtaa agagccccat 2700tatcttagcc taaaaaaacc
ttctctttgg aactttcagt aatacgctta actgctcatt 2760gctatattga
agtacggatt agaagccgcc gagcgggcga cagccctccg acggaagact
2820ctcctccgtg cgtcctggtc ttcaccggtc gcgttcctga aacgcagatg
tgcctcgcgc 2880cgcactgctc cgaacaataa agattctaca atactagctt
ttatggttat gaagaggaaa 2940aattggcagt aacctggccc cacaaacctt
caaatcaacg aatcaaatta acaaccatag 3000gataataatg cgattagttt
tttagcctta tttctggggt aattaatcag cgaagcgatg 3060atttttgatc
tattaacaga tatataaatg caaaagctgc ataaccactt taactaatac
3120tttcaacatt ttcggtttgt attacttctt attcaaatgt cataaaagta
tcaacaaaaa 3180attgttaata tacctctata ctttaacgtc aaggagaaaa
aactataatg tcattaccgt 3240tcttaacttc tgcaccggga aaggttatta
tttttggtga acactctgct gtgtacaaca 3300agcctgccgt cgctgctagt
gtgtctgcgt tgagaaccta cctgctaata agcgagtcat 3360ctgcaccaga
tactattgaa ttggacttcc cggacattag ctttaatcat aagtggtcca
3420tcaatgattt caatgccatc accgaggatc aagtaaactc ccaaaaattg
gccaaggctc 3480aacaagccac cgatggcttg tctcaggaac tcgttagtct
tttggatccg ttgttagctc 3540aactatccga atccttccac taccatgcag
cgttttgttt cctgtatatg tttgtttgcc 3600tatgccccca tgccaagaat
attaagtttt ctttaaagtc tactttaccc atcggtgctg 3660ggttgggctc
aagcgcctct atttctgtat cactggcctt agctatggcc tacttggggg
3720ggttaatagg atctaatgac ttggaaaagc tgtcagaaaa cgataagcat
atagtgaatc 3780aatgggcctt cataggtgaa aagtgtattc acggtacccc
ttcaggaata gataacgctg 3840tggccactta tggtaatgcc ctgctatttg
aaaaagactc acataatgga acaataaaca 3900caaacaattt taagttctta
gatgatttcc cagccattcc aatgatccta acctatacta 3960gaattccaag
gtctacaaaa gatcttgttg ctcgcgttcg tgtgttggtc accgagaaat
4020ttcctgaagt tatgaagcca attctagatg ccatgggtga atgtgcccta
caaggcttag 4080agatcatgac taagttaagt aaatgtaaag gcaccgatga
cgaggctgta gaaactaata 4140atgaactgta tgaacaacta ttggaattga
taagaataaa tcatggactg cttgtctcaa 4200tcggtgtttc tcatcctgga
ttagaactta ttaaaaatct gagcgatgat ttgagaattg 4260gctccacaaa
acttaccggt gctggtggcg gcggttgctc tttgactttg ttacgaagag
4320acattactca agagcaaatt gacagtttca aaaagaaatt gcaagatgat
tttagttacg 4380agacatttga aacagacttg ggtgggactg gctgctgttt
gttaagcgca aaaaatttga 4440ataaagatct taaaatcaaa tccctagtat
tccaattatt tgaaaataaa actaccacaa 4500agcaacaaat tgacgatcta
ttattgccag gaaacacgaa tttaccatgg acttcataag 4560ctaatttgcg
ataggcatta tttattagtt gtttttaatc ttaactgtgt atgaagtttt
4620atgtaataaa gatagaaaga gaaacaaaaa aaaatttttc gtagtatcaa
ttcagctttc 4680gaagacagaa tgaaatttaa gcagaccatc atcttgccct
gtgcttggcc cccagtgcag 4740cgaacgttat aaaaacgaat actgagtata
tatctatgta aaacaaccat atcatttctt 4800gttctgaact ttgtttacct
aactagtttt aaatttccct ttttcgtgca tgcgggtgtt 4860cttatttatt
agcatactac atttgaaata tcaaatttcc ttagtagaaa agtgagagaa
4920ggtgcactga cacaaaaaat aaaatgctac gtataactgt caaaactttg
cagcagcggg 4980catccttcca tcatagcttc aaacatatta gcgttcctga
tcttcatacc cgtgctcaaa 5040atgatcaaac aaactgttat tgccaagaaa
taaacgcaag gctgccttca aaaactgatc 5100cattagatcc tcatatcaag
cttcctcata gaacgcccaa ttacaataag catgttttgc 5160tgttatcacc
gggtgatagg tttgctcaac catggaaggt agcatggaat cataatttgg
5220atactaatac aaatcggcca tataatgcca ttagtaaatt gcgctcccat
ttaggtggtt 5280ctccaggcaa atttgaatac taataaatgc ggtgcatttg
caaaatgaat ttattccaag 5340gccaaaacaa cacgatgaat ggctttattt
ttttgttatt cctgacatga agctttatgt 5400aattaaggaa acggacatcg
aggaatttgc atctttttta gatgaaggag ctattcaagc 5460accaaagcta
tccttccagg attatttaag cggtaaggcc aaggcttccc aacaggttca
5520tgaagtgcat catagaaagc ttacaaggtt tcagggtgaa acttttctaa
gagattggaa 5580cttagtctgt gggcattata agagagatgc taagtgtgga
gaaatgggac ccgacataat 5640tgcagcattt caagatgaaa agctttttcc
tgagaataat ctagccttaa tttctcatat 5700tgggggtcat attt
5714207688DNAArtificial SequenceSynthetic Integration construct
i301 20gacggcacgg ccacgcgttt aaaccgccga gctattcgcg gaacattcta
gctcgtttgc 60atcttcttgc atttggtagg ttttcaatag ttcggtaata ttaacggata
cctactatta 120tcccctagta ggctcttttc acggagaaat tcgggagtgt
tttttttccg tgcgcatttt 180cttagctata ttcttccagc ttcgcctgct
gcccggtcat cgttcctgtc acgtagtttt 240tccggattcg tccggctcat
ataataccgc aataaacacg gaatatctcg ttccgcggat 300tcggttaaac
tctcggtcgc ggattatcac agagaaagct tcgtggagaa tttttccaga
360ttttccgctt tccccgatgt tggtatttcc ggaggtcatt atactgaccg
ccattataat 420gactgtacaa cgaccttctg gagaaagaaa caactcaata
acgatgtggg acattggggg 480cccactcaaa aaatctgggg actatatccc
cagagaattt ctccagaaga gaagaaaagt 540caaagttttt tttcgcttgg
gggttgcata taaagctcac acgcggccag ggggagccat 600gaaaaagcct
gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
660cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
gcttcgatgt 720aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
gatggtttct acaaagatcg 780ttatgtttat cggcactttg catcggccgc
gctcccgatt ccggaagtgc ttgacattgg 840ggaattcagc gagagcctga
cctattgcat ctcccgccgt gcacagggtg tcacgttgca 900agacctgcct
gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
960gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
cgcaaggaat 1020cggtcaatac actacatggc gtgatttcat atgcgcgatt
gctgatcccc atgtgtatca 1080ctggcaaact gtgatggacg acaccgtcag
tgcgtccgtc gcgcaggctc tcgatgagct 1140gatgctttgg gccgaggact
gccccgaagt ccggcacctc gtgcacgcgg atttcggctc 1200caacaatgtc
ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
1260gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
ggttggcttg 1320tatggagcag cagacgcgct acttcgagcg gaggcatccg
gagcttgcag gatcgccgcg 1380gctccgggcg tatatgctcc gcattggtct
tgaccaactc tatcagagct tggttgacgg 1440caatttcgat gatgcagctt
gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc 1500cgggactgtc
gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
1560tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
gggcaaagga 1620atagcgctcg tccaacgccg gcggacctcg ctcgtccaac
gccggcggac ctcttttaat 1680tctgctgtaa cccgtacatg cccaaaatag
ggggcgggtt acacagaata tataacatcg 1740taggtgtctg ggtgaacagt
ttattcctgg catccactaa atataatgga gcccgctttt 1800taagctggca
tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
1860catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca
caaacaggca 1920aaaaacgggc acaacctcaa
tggagtgatg caacctgcct ggagtaaatg atgacacaag 1980gcaattgacc
cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
2040ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa
ttattcccct 2100acttgactaa taagtatata aagacggtag gtattgattg
taattctgta aatctatttc 2160ttaaacttct taaattctac ttttatagtt
agtctttttt ttagttttaa aacaccaaga 2220acttagtttc gatccccgcg
tgcttggccg gccgtatccc cgcgtgcttg gccggccgta 2280tgtctcagaa
cgtttacatt gtatcgactg ccagaacccc aattggttca ttccagggtt
2340ctctatcctc caagacagca gtggaattgg gtgctgttgc tttaaaaggc
gccttggcta 2400aggttccaga attggatgca tccaaggatt ttgacgaaat
tatttttggt aacgttcttt 2460ctgccaattt gggccaagct ccggccagac
aagttgcttt ggctgccggt ttgagtaatc 2520atatcgttgc aagcacagtt
aacaaggtct gtgcatccgc tatgaaggca atcattttgg 2580gtgctcaatc
catcaaatgt ggtaatgctg atgttgtcgt agctggtggt tgtgaatcta
2640tgactaacgc accatactac atgccagcag cccgtgcggg tgccaaattt
ggccaaactg 2700ttcttgttga tggtgtcgaa agagatgggt tgaacgatgc
gtacgatggt ctagccatgg 2760gtgtacacgc agaaaagtgt gcccgtgatt
gggatattac tagagaacaa caagacaatt 2820ttgccatcga atcctaccaa
aaatctcaaa aatctcaaaa ggaaggtaaa ttcgacaatg 2880aaattgtacc
tgttaccatt aagggattta gaggtaagcc tgatactcaa gtcacgaagg
2940acgaggaacc tgctagatta cacgttgaaa aattgagatc tgcaaggact
gttttccaaa 3000aagaaaacgg tactgttact gccgctaacg cttctccaat
caacgatggt gctgcagccg 3060tcatcttggt ttccgaaaaa gttttgaagg
aaaagaattt gaagcctttg gctattatca 3120aaggttgggg tgaggccgct
catcaaccag ctgattttac atgggctcca tctcttgcag 3180ttccaaaggc
tttgaaacat gctggcatcg aagacatcaa ttctgttgat tactttgaat
3240tcaatgaagc cttttcggtt gtcggtttgg tgaacactaa gattttgaag
ctagacccat 3300ctaaggttaa tgtatatggt ggtgctgttg ctctaggtca
cccattgggt tgttctggtg 3360ctagagtggt tgttacactg ctatccatct
tacagcaaga aggaggtaag atcggtgttg 3420ccgccatttg taatggtggt
ggtggtgctt cctctattgt cattgaaaag atatgattac 3480gttctgcgat
tttctcatga tctttttcat aaaatacata aatatataaa tggctttatg
3540tataacaggc ataatttaaa gttttatttg cgattcatcg tttttcaggt
actcaaacgc 3600tgaggtgtgc cttttgactt acttttccgc cttggcaagc
tggccgggtg atacttgcac 3660aagttccact aattactgac atttgtggta
ttaactcgtt tgactgctct acaattgtag 3720gatgttaatc aatgtcttgg
ctgcctaacc tgcaggccgc gagcgccgat atgctatgta 3780atagacaata
aaaccatgtt tatataaaaa aaattcaaaa tagaaaacga ttctgtacaa
3840ggagtatttt ttttttgttc tagtgtgttt atattatcct tggctaagag
gcactgcgta 3900tacttcaagg tacccctgtg ttttgaaaaa aaacaacagt
aaaataggaa ctccgcgagg 3960ttcaggaacc tgaaacaaaa tcaataaaaa
cattatatgc gtttcgaaca aaattaaaga 4020aaaagaataa atatagatta
aaaaaaaaaa gaagaaatta aaagaatttc tactaaatcc 4080caattgttat
atatttgtta aatgccaaaa aagtttataa aaaatttaga atgtataaat
4140aataataaac taagtaacgc gatcgccgac gccgccgata tctccctcgc
cagcggccgc 4200cttatggcta agaatgttgg aattttggcc atggacatct
acttcccacc aacttgtgtt 4260cagcaggagg ctttagaagc acatgacgga
gcctcaaagg gtaagtacac aatcggatta 4320ggacaggatt gcttagcatt
ctgcactgaa ttggaggacg tcatctcaat gtctttcaac 4380gccgtcacct
cattgttaga gaagtacaaa atcgacccaa accagatcgg aaggttggaa
4440gtcggttctg aaaccgtcat cgacaagtct aaatcaatca agactttcgt
tatgcagttg 4500ttcgaaaagt gcggtaatac tgacgtcgag ggtgtagact
ctactaacgc ttgttatggt 4560ggtaccgcag ctttattgaa ctgcgtaaac
tgggttgagt caaactcatg ggatggtagg 4620tacggattag tcatttgcac
cgattctgcc gtctacgccg agggtccagc aaggccaacc 4680ggtggagctg
cagctattgc tatgttaatc ggaccagatg cccctatagt cttcgagtct
4740aagttgaggg gttcacacat ccctaacgtc tacgacttct acaagccaaa
cttggcctca 4800gagtatccag ttgtcgacgg aaagttatct cagacatgct
acttgatggc cttagattca 4860tgttacaagc acttatgcaa caagttcgaa
aagttggagg gaaaggagtt ctcaattaac 4920gacgccgact acttcgtttt
tcactctcca tacaacaaat tggtccagaa gtcattcgcc 4980aggttattgt
acaacgattt tttgagaaac gcatcatcta tcgatgaggc cgccaaggag
5040aaattcaccc catattcttc tttgtcattg gacgagtctt accagtctag
ggacttggag 5100aaggtatcac agcaattggc taaaaccttc tatgacgcca
aagttcagcc aaccaccttg 5160gtccctaaac aggtcggaaa tatgtatact
gcatctttgt atgccgcctt tgcctctttg 5220atccacaaca agcacaacga
tttagtcgga aaaagggttg tcatgttttc ttacggtgcc 5280ggatctactg
ccactatgtt ctcattgagg ttatgcgaaa accagtcacc attttcattg
5340tctaacatcg cctcagtcat ggacgtaggt gtctcacctg agaagttcgt
agaaaccatg 5400aagttgatgg agcacagata cggtgccaaa gaattcgtca
cttcaaaaga gggaatcttg 5460gatttgttgg ccccaggaac ctactatttg
aaggaggtcg actctttgta cagaaggttc 5520tatggaaaga agggagacga
cggatctgtc gcaaacggtc agtaaatcgg cggcgtcggc 5580gatcgcgtta
aggcggccgc tggcgaggga gatatttcaa cctgggccta acagtaaaga
5640tatcctcctc aaaactggtg cacttaatcg ctgaatttgt tctggcttct
cttctttttc 5700tttattcccc ccatgggcca aaaaaaatag tactatcagg
aatttggcgc cgggtcacga 5760tatacgtgta cagtgaccta ggcgacgcca
caaggaaaaa ggaaaaaaac agaaaaaaca 5820acaaaaacta aaacaaacac
gaaaacttta atagatctaa gtgaagtagt ggtgaggcaa 5880ttggagtgac
atagcagcta ctacaactac aaaaaaggcg cgccacggtc gtgcggatat
5940gaaagaggtc gttatagctt ctgccgtcag gaccgccatc ggatcttacg
gtaagtcatt 6000aaaggacgtc cctgccgttg atttaggagc caccgcaatt
aaagaggccg ttaaaaaggc 6060aggtataaag ccagaggacg tcaacgaggt
catcttggga aatgtcttac aagccggatt 6120aggtcaaaac ccagcaagac
aagcatcatt caaagccggt ttacctgtcg agatacctgc 6180aatgaccatc
aacaaggttt gcggttcagg attaaggacc gtttctttag cagcacagat
6240cattaaggct ggagatgcag acgttatcat tgctggtggt atggaaaaca
tgtcaagagc 6300cccatacttg gctaataacg ccaggtgggg atataggatg
ggaaacgcca agtttgtcga 6360cgaaatgatt actgacggat tgtgggacgc
cttcaatgac tatcacatgg gtataaccgc 6420agaaaacatt gccgagaggt
ggaatatctc aagagaagaa caggatgagt ttgcattggc 6480ctcacagaaa
aaagcagagg aggcaataaa gtcaggtcag tttaaggatg aaatcgtccc
6540agtcgtcatc aagggaagaa agggtgagac agttgtcgac accgacgaac
accctagatt 6600tggttcaacc atcgagggat tagcaaagtt gaagccagcc
ttcaagaaag acggaaccgt 6660aaccgccggt aatgcatctg gattgaacga
ttgcgcagca gttttggtca taatgtcagc 6720cgagaaagct aaggagttgg
gtgtcaagcc attggcaaaa attgtttcat acggatcagc 6780cggtgtcgac
cctgccatca tgggttacgg acctttttac gccaccaagg ctgcaatcga
6840aaaggccggt tggaccgtag atgaattgga tttgatcgag tcaaacgagg
cctttgccgc 6900ccaatcattg gctgtcgcca aggacttgaa gttcgacatg
aacaaggtca acgtcaacgg 6960tggtgccatc gcattgggtc accctatcgg
agcctctggt gccaggatct tggttacctt 7020ggtccacgcc atgcagaaga
gggacgcaaa gaagggtttg gccaccttgt gcatcggtgg 7080aggtcaggga
acagctatct tgttagagaa atgcagcccc tcagcccccc tagcgtcgaa
7140taaaagacat tggtacatga tatcaaacag aattttaaca tttcttgatc
cagtttgtaa 7200acaaaacaaa caatttttct accatttaac ttcataccat
cggcgagagc cgaacaggaa 7260aaaaaagaag tctccggtta tcgtaagcag
tatcaaataa taagaatgta tgtgtgtgca 7320atttgttata cccacgaaga
agtgcgcagt agagttagaa aaccaactga gtaatcttta 7380ctcccgacaa
tcgtccaata atcctcttgt tgctaggaac gtgatgatgg atttcgtttg
7440aaatccggac ggaaaactca aaagaagtcc aaccaccaac cattttcgag
cctcaagaat 7500ctctaagcag gtttctttac taaggggatg gcctttctgt
cctggacatt ttttccttcc 7560ttttttcatt tccttgaaag gaacagattt
tttttgactt ttgccacaca gctgcactat 7620ctcaacccct tttacatttt
aagttttcgg gttgaatggc cggtgtttaa accccagcgc 7680ctggcggg
7688215025DNAArtificial SequenceSynthetic Integration construct
i476 21caggatccga cggcacggcc acgcgtttaa accgcctggg ataggatagt
agcaactctt 60ggaggagagc attgtcagtt gtccagtctc tgaagttaag tagtaagttt
gcggagtcaa 120agggggatgg cttttgccat ttgtgagagt tgtgcggcag
catcttattc aaatagagct 180gtattctgaa gacctcttgt agaacatcat
ccatactaaa aagtaaatcg tcctgtccca 240ttacgagctg tattagtgct
gtgaccctct gtatatttac gttgccatga agaaggtaat 300gggcgatatt
ttgatacaat tcctgagttg catgttggat tgagtttacg aagggtcgcc
360agacggccag aaacctccag gcggagttaa caactagtaa tacggcatcc
atgtttgcat 420cagcgccgag cctataccag tcactgagta gacgttttct
tgctcttttt atgtcctgac 480ttcttttgac gagggggcat tctctagaga
cacaggcagt tgcttccagc aactgccgta 540cggccgttct catgctgtcg
aggatttttt ttgggacgat attgtcatta tagggcagtg 600tgtgacttat
gaattgttgt agaaggacgt ctgtgatgtt ggagatatgt attttgttaa
660ctcttcttga gacgatttgg ccctggatag cgaagcgtgc ggttacaaat
aggtcgtctt 720gttcaagaag gtaggcgagg acattatcta tcagtacaaa
catcttagta gtgtctgagg 780agagggttga ttgtttatgt atttttgcga
aatatatata tatatattct acacagatat 840atacatattt gtttttcggg
ctcattcttt cttctttgcc agaggctcac cgctcaagag 900gtccgctaat
tctggagcga ttgttattgt tttttctttt cttcttctat tcgaaaccca
960gtttttgatt tgaatgcgag ataaactggt attcttcatt agattctcta
ggcccttggt 1020atctagatat gggttctcga tgttctttgc aaaccaactt
tctagtattc ggacattttc 1080ttttgtaaac cggtgtcctc tgtaaggttt
agtacttttg tttatcatat cttgagttac 1140cacattaaat accaacccat
ccgccgattt atttttctgt gtaagttgat aattacttct 1200atcgttttct
atgctgcgca tttctttgag taatacagta atggtagtag tgagttgaga
1260tgttgtttgc aacaacttct tctcctcatc actaatctta cggtttttgt
tggccctaga 1320taagaatcct aatatatccc ttaattcaac ttcttcttct
gttgttacac tctctggtaa 1380cttaggtaaa ttacagcaaa tagaaaagag
ctttttattt atgtctagta tgctggattt 1440aaactcatct gtgatttgtg
gatttaaaag gtctttaatg ggtattttat tcattttttc 1500ttgcttatct
tccttttttt cttgcccact tctaagctga tttcaatctc tcctttatat
1560atatttttaa gttccaacat tttatgtttc aaaacattaa tgatgtctgg
gttttgtttg 1620ggatgcaatt tattgcttcc caatgtagaa aagtacatca
tatgaaacaa cttaaactct 1680taactacttc ttttaacctt cactttttat
gaaatgtatc aaccatatat aataacttaa 1740tagacgacat tcacaatatg
tttacttcga agcctgcttt caaaattaag aacaaagcat 1800ccaaatcata
cagaaacaca gcggtttcaa aaaagctgaa agaaaaacgt ctagctgagc
1860atgtgaggcc aagctgcttc aatattattc gaccactcaa gaaagatatc
cagattcctg 1920ttccttcctc tcgattttta aataaaatcc aaattcacag
gatagcgtct ggaagtcaaa 1980atactcagtt tcgacagttc aataagacat
ctataaaatc ttcaaagaaa tatttaaact 2040catttatggc ttttagagca
tattactcac agtttggctc cggtgtaaaa caaaatgtct 2100tgtcttctct
gctcgctgaa gaatggcacg cggacaaaat gcagcacgga atatgggact
2160acttcgcgca acagtataat tttataaacc ctggttttgg ttttgtagag
tggttgacga 2220ataattatgc tgaagtacgt ggtgacggat attgggaaga
tgtgtttgta catttggcct 2280tatagagtgt ggtcgtggcg gaggttgttt
atctttcgag tactgaatgt tgtcagtata 2340gctatcctat ttgaaactcc
ccatcgtctt gctcttgttc ccaatgtttg tttatacact 2400catatggcta
tacccttatc tacttgcctc ttttgtttat gtctatgtat ttgtataaaa
2460tatgatatta ctcagactca agcaaacaat caatgctcac acgcggccag
ggggagcctc 2520gacactagta atacacatca tcgtcctaca agttcatcaa
agtgttggac agacaactat 2580accagcatgg atctcttgta tcggttcttt
tctcccgctc tctcgcaata acaatgaaca 2640ctgggtcaat catagcctac
acaggtgaac agagtagcgt ttatacaggg tttatacggt 2700gattcctacg
gcaaaaattt ttcatttcta aaaaaaaaaa gaaaaatttt tctttccaac
2760gctagaagga aaagaaaaat ctaattaaat tgatttggtg attttctgag
agttcccttt 2820ttcatatatc gaattttgaa tataaaagga gatcgaaaaa
atttttctat tcaatctgtt 2880ttctggtttt atttgatagt ttttttgtgt
attattatta tggattagta ctggtttata 2940tgggtttttc tgtataactt
ctttttattt tagtttgttt aatcttattt tgagttacat 3000tatagttccc
taactgcaag agaagtaaca ttaaaaatga aaaagcctga actcaccgcg
3060acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
gatgcagctc 3120tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
gagggcgtgg atatgtcctg 3180cgggtaaata gctgcgccga tggtttctac
aaagatcgtt atgtttatcg gcactttgca 3240tcggccgcgc tcccgattcc
ggaagtgctt gacattgggg aattcagcga gagcctgacc 3300tattgcatct
cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
3360cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
cgatcttagc 3420cagacgagcg ggttcggccc attcggaccg caaggaatcg
gtcaatacac tacatggcgt 3480gatttcatat gcgcgattgc tgatccccat
gtgtatcact ggcaaactgt gatggacgac 3540accgtcagtg cgtccgtcgc
gcaggctctc gatgagctga tgctttgggc cgaggactgc 3600cccgaagtcc
ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
3660ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
ccaatacgag 3720gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
tggagcagca gacgcgctac 3780ttcgagcgga ggcatccgga gcttgcagga
tcgccgcggc tccgggcgta tatgctccgc 3840attggtcttg accaactcta
tcagagcttg gttgacggca atttcgatga tgcagcttgg 3900gcgcagggtc
gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
3960atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
cgccgatagt 4020ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
aggtttaact tgatactact 4080agattttttc tcttcattta taaaattttt
ggttataatt gaagctttag aagtatgaaa 4140aaatcctttt ttttcattct
ttgcaaccaa aataagaagc ttcttttatt cattgaaatg 4200atgaatataa
acctaacaaa agaaaaagac tcgaatatca aacattaaaa aaaaataaaa
4260gaggttatct gttttcccat ttagttggag tttgcatttt ctaatagata
gaactctcaa 4320ttaatgtgga tttagtttct ctgttcgttt ttttttgttt
tgttctcact gtatttacat 4380ttctatttag tatttagtta ttcatataat
cttaacttct cgaggagctc cgctcgtcca 4440acgccggcgg acctcggagg
ttgtttatct ttcgagtact gaatgttgtc agtatagcta 4500tcctatttga
aactccccat cgtcttgctc ttgttcccaa tgtttgttta tacactcata
4560tggctatacc cttatctact tgcctctttt gtttatgtct atgtatttgt
ataaaatatg 4620atattactca gactcaagca aacaatcaat tcttagcatc
attctttgtt cttatcttaa 4680ccataaacga tcttgatgtg acttttgtaa
tttgaacgaa ttggctatac gggacggatg 4740acaaatgcac cattactcta
ggttgttgtt ggatcttaac aaaccgtaaa ggtaaactgc 4800ccatgcggtt
cacatgactt ttgactttcc tttgtttgct agttaccttc ggcttcacaa
4860tttgtttttc cacttttcta acaggtttat cacctttcaa acttatcttt
atcttattcg 4920ccttcttggg tgcctccaca gtagaggtta cttccttttt
aatatgtact tttaggatac 4980tttcacgctt tataacacgg tgtttaaacc
ccagcgcctg gcggg 5025223665DNAArtificial SequenceSynthetic
Integration construct i477 22agctcgagga cggcacggcc acgcgtttaa
accgccaagc ttttcaattc atcttttttt 60tttttgttct tttttttgat tccggtttct
ttgaaatttt tttgattcgg taatctccga 120gcagaaggaa gaacgaagga
aggagcacag acttagattg gtatatatac gcatatgtgg 180tgttgaagaa
acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
240caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
ctactcatcc 300tagtcctgtt gctgccaagc tatttaatat catgcacgaa
aagcaaacaa acttgtgtgc 360ttcattggat gttcgtacca ccaaggaatt
actggagtta gttgaagcat taggtcccaa 420aatttgttta ctaaaaacac
atgtggatat cttgactgat ttttccatgg agggcacagt 480taagccgcta
aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
540tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
gaatagcaga 600atgggcagac attacgaatg cacacggtgt ggtgggccca
ggtattgtta gcggtttgaa 660gcaggcggca gaagaagtaa caaaggaacc
tagaggcctt ttgatgttag cagaattgtc 720atgcaagggc tccctatcta
ctggagaata tactaagggt actgttgaca ttgcgaagag 780tgacaaagat
tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
840ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
acgcattggg 900tcaacagtat agaaccgtgg atgatgtggt ctctacagga
tctgacatta ttattgttgg 960aagcgctcgt ccaacgccgg cggacctatg
gcgcaagttt tccgctttgt aatatatatt 1020tatacccctt tcttctctcc
cctgcaatat aatagtttaa ttctaatatt aataatatcc 1080tatattttct
tcatttaccg gcgcactctc gcccgaacga cctcaaaatg tctgctacat
1140tcataataac caaaagctca taactttttt ttttgaacct gaatatatat
acatcacata 1200tcactgctgg tccttgccga ccagcgtata caatctcgat
agttggtttc ccgttctttc 1260cactcccgtc atggactaca acaagagatc
ttcggtctca accgtgccta atgcagctcc 1320cataagagtc ggattcgtcg
gtctcaacgc agccaaagga tgggcaatca agacacatta 1380ccccgccata
ctgcaactat cgtcacaatt tcaaatcact gccttataca gtccaaaaat
1440tgagacttct attgccacca tccagcgtct aaaattgagt aatgccactg
cttttcccac 1500tttagagtca tttgcatcat cttccactat agatatgata
gtgatagcta tccaagtggc 1560cagtcattat gacgttgtta tgcctctctt
ggaattctcc aaaaataatc cgaacctcaa 1620gtatcttttc gtagaatggg
cccttgcatg ttcactagat caagccgaat ccatttataa 1680ggctgctgct
gaacgtgggg ttcaaaccat catctcttta caaggtcgta aatcaccata
1740tattttgaga gcaaaagaat taatatctca aggctatatc ggcgacatta
attctatcga 1800gattgctgga aatggcggtt ggtacggcta cgaaaggcct
gttaaatcac caaaatacat 1860ctatgaaatc gggaacggtg tagatctggt
aaccacaaca tttggtcaca caatcgatat 1920tttacaatac atgacaagtt
cgtacttttc caggataaat gcaatggttt tcaataatat 1980tccagagcaa
gagctgatag atgagcgtgg taaccgattg ggccagcgag tcccaaagac
2040agtaccggat catcttttat tccaaggcac attgttaaat ggcaatgttc
cagtgtcatg 2100cagtttcaaa ggtggcaaac ctaccaaaaa atttaccaaa
aatttggtca ttgatattca 2160cggtaccaag ggagatttga aacttgaagg
cgatgccgga ttcgcagaaa tttcaaatct 2220ggtcctttac tacagtggaa
ctagagcaaa cgacttcccg ctagctaatg gacaacaagc 2280tcctttagac
ccggggtatg atgcaggtaa agaaatcatg gaagtatatc atttacgaaa
2340ttataatgcc attgtcggta atattcatcg actgtatcaa tctatctctg
acttccactt 2400caatacaaag aaaattcctg aattaccctc acaatttgta
atgcaaggtt tcgatttcga 2460aggctttccc accttgatgg atgctctgat
attacacagg ttaatcgaga gcgtttataa 2520aagtaacatg atgggctcca
cattaaacgt tagcaatatc tcgcattata gtttataaaa 2580gcatcttgcc
ctgtgcttgg cccccagtgc agcgaacgtt ataaaaacga atactgagta
2640tatatctatg taaaacaacc atatcatttc ttgttctgaa ctttgtttac
ctaactagtt 2700ttaaatttcc ctttttcgtg catgcgggtg ttcttattta
ttagcatact acatttgaaa 2760tatcaaattt ccttagtaga aaagtgagag
aaggtgcact gacacaaaaa ataaaatccc 2820cgcgtgcttg gccggccgtc
ttcattggat gttcgtacca ccaaggaatt actggagtta 2880gttgaagcat
taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat
2940ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
caatttttta 3000ctcttcgaag acagaaaatt tgctgacatt ggtaatacag
tcaaattgca gtactctgcg 3060ggtgtataca gaatagcaga atgggcagac
attacgaatg cacacggtgt ggtgggccca 3120ggtattgtta gcggtttgaa
gcaggcggca gaagaagtaa caaaggaacc tagaggcctt 3180ttgatgttag
cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt
3240actgttgaca ttgcgaagag tgacaaagat tttgttatcg gctttattgc
tcaaagagac 3300atgggtggaa gagatgaagg ttacgattgg ttgattatga
cacccggtgt gggtttagat 3360gacaagggag acgcattggg tcaacagtat
agaaccgtgg atgatgtggt ctctacagga 3420tctgacatta ttattgttgg
aagaggacta tttgcaaagg gaagggatgc taaggtagag 3480ggtgaacgtt
acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac
3540taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
agcttcaatt 3600taattatatc agttattacc cgggaatctc ggtgtttaaa
ccccagcgcc tggcgggtct 3660agatc 36652310623DNAArtificial
SequenceSynthetic Integration construct i94 23atgagtgata gggaattcgt
cacggtagat cccgtcacta tcataatcaa agaatgcatt 60aatttatcga cagcgatgcg
gaaatactct aaatttacct ctcaatctgg agtggccgct 120ttgctggggg
gaggaagtga aatatttagc aatcaagatg actacttggc tcacacattc
180aacaatttga ataccaacaa gcacaatgat ccatttttat ctggattcat
tcagttaaga 240cttatgttga ataaactgaa aaatctagat aatatagatt
cactaaccat attgcagcca 300tttttattaa ttgtgagtac
aagttccatt tctggttaca tcacttccct ggccctggac 360tctttgcaga
aattctttac cttgaatatc atcaatgaat catcgcaaaa ctatattggt
420gcacacaggg cgacggtaaa tgctctaaca cattgtaggt ttgaaggatc
tcaacaactt 480tctgatgatt cagttctttt gaaagtcgtg tttttactgc
gttcaatcgt cgactcacct 540tacggagatt tattatcaaa ctctatcata
tatgacgtat tgcaaacgat tctttcattg 600gcttgtaata acagaaggag
cgaagtcctt aggaatgctg cacaatcaac aatgatagcc 660gttaccgtaa
agattttctc aaaactaaag actattgagc ctgttaatgt gaatcaaata
720tacatcaatg atgaaagtta cacaaatgat gtattgaagg ccgatacaat
tggcacaaat 780gtagaatcca aagaagaagg aagtcaagaa gatcccatcg
gcatgaaagt gaataatgag 840gaagctatta gcgaggacga tggcattgaa
gaagagcata ttcattcaga gaagagcaca 900aatggcgccg aacaactaga
tattgtgcaa aaaacaacaa gatcaaattc caggatccaa 960gcgtatgctg
atgataacta tggattgccc gtggttaggc aatatttaaa cttattacta
1020tcattgattg cgccagaaaa tgaattaaaa cattcatact ccactagaat
atttggccta 1080gagttaattc aaacggcatt agaaatttca ggtgatcgat
tgcagctata cccacggctt 1140tttacactga tatcagatcc tattttcaaa
agcattttgt ttatcataca gaacactaca 1200aaattatcac tacttcaagc
tacattgcag ctatttacta ctctagttgt tatattgggc 1260aacaacttac
aattacagat cgagctcact ctaacaagaa tattttctat tcttttagat
1320gatggtaccg caaataactc gagttctgaa aataagaaca agccatcaat
aataaaggaa 1380cttctaattg agcaaatatc catcttatgg actaggtcgc
catctttttt tacttctact 1440tttatcaatt tcgattgtaa tctcgatagg
gcagacgttt ccataaactt tttgaaggct 1500ttgactaaat tggccttacc
agaatccgcc ttaactacca cagaaagtgt accacccatt 1560tgccttgagg
gattggtctc cctagtcgat gatatgttcg atcacatgaa ggacattgac
1620agagaagaat ttggcaggca aaagaatgaa atggaaatct taaaaaagag
ggaccgtaaa 1680acagagttta ttgaatgtac caatgcattc aatgaaaagc
ccaaaaaggg tattccgatg 1740ttaatagaaa aaggtttcat tgcttccgac
tccgataaag atattgcgga gtttcttttc 1800aataataaca accgtatgaa
taaaaaaaca atcggtttgc tactttgcca tccggacaaa 1860gtaagcttgt
tgaatgaata tattcgtttg tttgattttt cagggttaag ggtcgatgaa
1920gctattagaa ttttgttgac gaaatttagg ttgcctggtg aatcgcaaca
aattgaaaga 1980atcatcgaag ccttctcgtc tgcgtattgt gaaaatcaag
attacgatcc atccaaaatc 2040agtgacaacg cggaggatga catttctact
gttcaaccag acgctgattc tgttttcatt 2100ttaagttatt caattattat
gttgaacact gacctacata accctcaagt gaaggaacac 2160atgtcatttg
aagattactc tggtaactta aagggatgct gtaatcacaa agacttccca
2220ttctggtatt tggatagaat ttactgttca atcagagata aagaaattgt
tatgcctgaa 2280gagcaccacg gcaacgaaaa gtggtttgaa gatgcttgga
ataacttgat atcttcaact 2340actgttataa ctgaaataaa aaaagacaca
caatctgtca tggataaatt aacacccttg 2400gagcttttga actttgatag
agcaattttt aaacaagttg gcccaagtat tgtcagtact 2460ttattcaaca
tttacgtagt tgcatctgat gaccatatat ctaccagaat gataacaagt
2520ttggacaaat gttcctatat ttccgcattt tttgacttca aagatctctt
taatgatata 2580ctaaactcca ttgctaaggg cactactttg attaattcaa
gccatgacga tgaactttca 2640actttagctt ttgaatatgg cccaatgcca
ctggtgcaaa ttaaattcga agacactaac 2700actgagatcc cggttagtac
agatgctgtt agatttggta gatcatttaa gggtcaacta 2760aatacagttg
tttttttccg gattattcgc aggaacaaag atcctaaaat tttctccaag
2820gaattatggt taaacattgt taatattata ctaacattgt acgaagactt
gattttgtct 2880cctgatattt tccctgattt acaaaaaaga ctgaaattaa
gcaacttgcc taagccatct 2940cctgaaattt ctattaacaa gagcaaagaa
agcaaaggtc tcttatcaac atttgcttct 3000tatttaaaag gtgatgaaga
acccacagaa gaggaaatca aatcctcaaa aaaagcgatg 3060gagtgcataa
agtcgagtaa tattgccgcc tctgtctttg gaaatgaatc aaatataaca
3120gcggatttaa taaaaacttt actagactcc gccaaaactg agaaaaacgc
agataattcc 3180aggtattttg aagcagaact tttatttatc atcgaattga
ctattgcatt atttctattt 3240tgcaaagagg agaaagaatt aggaaagttc
atacttcaaa aagttttcca actttctcac 3300acgaaaggcc tcacgaaaag
gactgttcgt agaatgctaa catacaaaat tttgttaatt 3360tcgttatgtg
cggatcagac ggagtacttg tccaaattaa taaacgatga gctgttaaaa
3420aagggggata tttttaccca aaaatttttt gcaactaatc aaggtaagga
atttttgaag 3480agactatttt cattgaccga atcagagttt tatagaggat
ttttactagg aaatgagaat 3540ttttggaaat ttttaagaaa agttacagca
atgaaagagc agagcgagag catttttgaa 3600tatttaaatg aatcgatcaa
gacagacagc aatattttga caaatgagaa cttcatgtgg 3660gtcctaggac
tattagatga aatttcatca atgggtgccg ttggaaatca ctgggaaata
3720gaatacaaga aattgacaga aagtggtcat aaaattgata aggagaatcc
atacaagaaa 3780tcgatcgaat tatcattgaa atccattcaa ctaacatcac
acttgctgga agataataac 3840gatctgcgta aaaacgagat attcgctatt
attcaagctt tggcacatca atgcatcaat 3900ccgtgtaagc agataagtga
atttgcagtg gtaacgctag agcagacgct catcaataaa 3960atcgaaattc
caactaatga gatggaatcg gtagaagaat taattgaggg cggattacta
4020ccgttgctaa attcgagtga aacacaggaa gaccagaaaa tcctcatttc
atccatatta 4080acaataattt caaatgttta tttgcattat ttgaaactag
ggaagacaag caacgaaacg 4140tttttgaaaa ttttgagtat tttcaataaa
tttgtagagg actcagatat tgaaaaaaag 4200ctacagcaat taatacttga
taagaagagt attgagaagg gcaacggttc atcatctcat 4260ggatctgcac
atgaacaaac accagagtca aacgacgttg aaattgaggc tactgcgcca
4320attgatgaca atacagacga tgataacaaa ccgaagttat ctgatgtaga
aaaggattaa 4380agatgctaag agatagtgat gatatttcat aaataatgta
attctatata tgttaattac 4440cttttttgcg aggcatattt atggtgaagg
ataagttttg accatcaaag aaggttaatg 4500tggctgtggt ttcagggtcc
atacccggga gttatgacaa ttacaacaac agaattcttt 4560ctatatatgc
acgaacttgt aatatggaag aaattatgac gtacaaacta taaagtaaat
4620attttacgta acacatggtg ctgttgtgct tctttttcaa gagaatacca
atgacgtatg 4680actaagttta ggatttaatg caggtgacgg acccatcttt
caaacgattt atatcagtgg 4740cgtccaaatt gttaggtttt gttggttcag
caggtttcct gttgtgggtc atatgacttt 4800gaaccaaatg gccggctgct
agggcagcac ataaggataa ttcacctgcc aagacggcac 4860aggcaactat
tcttgctaat tgacgtgcgt tggtaccagg agcggtagca tgtgggcctc
4920ttacacctaa taagtccaac atggcacctt gtggttctag aacagtacca
ccaccgatgg 4980tacctacttc gatggatggc atggatacgg aaattctcaa
atcaccgtcc acttctttca 5040tcaatgttat acagttggaa ctttcgacat
tttgtgcagg atcttgtcct aatgccaaga 5100aaacagctgt cactaaatta
gctgcatgtg cgttaaatcc accaacagac ccagccattg 5160cagatccaac
caaattctta gcaatgttca actcaaccaa tgcggaaaca tcacttttta
5220acacttttct gacaacatca ccaggaatag tagcttctgc gacgacactc
ttaccacgac 5280cttcgatcca gttgatggca gctggttttt tgtcggtaca
gtagttacca gaaacggaga 5340caacctccat atcttcccag ccatactctt
ctaccatttg ctttaatgag tattcgacac 5400ccttagaaat catattcata
cccattgcgt caccagtagt tgttctaaat ctcatgaaga 5460gtaaatctcc
tgctagacaa gtttgaatat gttgcagacg tgcaaatctt gatgtagagt
5520taaaagcttt tttaattgcg ttttgtccct cttctgagtc taaccatatc
ttacaggcac 5580cagatctttt caaagttggg aaacggacta ctgggcctct
tgtcatacca tccttagtta 5640aaacagttgt tgcaccaccg ccagcattga
ttgccttaca gccacgcatg gcagaagcta 5700ccaaacaacc ctctgtagtt
gccattggta tatgataaga tgtaccatcg ataaccaagg 5760ggcctataac
accaacgggc aaaggcatgt aacctataac attttcacaa caagcgccaa
5820atacgcggtc gtagtcataa tttttatatg gtaaacgatc agatgctaat
acaggagctt 5880ctgccaaaat tgaaagagcc ttcctacgta ccgcaaccgc
tctcgtagta tcacctaatt 5940ttttctccaa agcgtacaaa ggtaacttac
cgtgaataac caaggcagcg acctctttgt 6000tcttcaattg ttttgtattt
ccactactta ataatgcttc taattcttct aaaggacgta 6060ttttcttatc
caagctttca atatcgcggg aatcatcttc ctcactagat gatgaaggtc
6120ctgatgagct cgattgcgca gatgataaac ttttgacttt cgatccagaa
atgactgttt 6180tattggttaa aactggtgta gaagcctttt gtacaggagc
agtaaaagac ttcttggtga 6240cttcagtctt caccaattgg tctgcagcca
ttatagtttt ttctccttga cgttaaagta 6300tagaggtata ttaacaattt
tttgttgata cttttatgac atttgaataa gaagtaatac 6360aaaccgaaaa
tgttgaaagt attagttaaa gtggttatgc agcttttgca tttatatatc
6420tgttaataga tcaaaaatca tcgcttcgct gattaattac cccagaaata
aggctaaaaa 6480actaatcgca ttattatcct atggttgtta atttgattcg
ttgatttgaa ggtttgtggg 6540gccaggttac tgccaatttt tcctcttcat
aaccataaaa gctagtattg tagaatcttt 6600attgttcgga gcagtgcggc
gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga 6660ccaggacgca
cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa
6720tccgtacttc aatatagcaa tgagcagtta agcgtattac tgaaagttcc
aaagagaagg 6780tttttttagg ctaagataat ggggctcttt acatttccac
aacatataag taagattaga 6840tatggatatg tatatggtgg tattgccatg
taatatgatt attaaacttc tttgcgtcca 6900tccaaaaaaa aagtaagaat
ttttgaaaat tcaatataaa tgaaactctc aactaaactt 6960tgttggtgtg
gtattaaagg aagacttagg ccgcaaaagc aacaacaatt acacaataca
7020aacttgcaaa tgactgaact aaaaaaacaa aagaccgctg aacaaaaaac
cagacctcaa 7080aatgtcggta ttaaaggtat ccaaatttac atcccaactc
aatgtgtcaa ccaatctgag 7140ctagagaaat ttgatggcgt ttctcaaggt
aaatacacaa ttggtctggg ccaaaccaac 7200atgtcttttg tcaatgacag
agaagatatc tactcgatgt ccctaactgt tttgtctaag 7260ttgatcaaga
gttacaacat cgacaccaac aaaattggta gattagaagt cggtactgaa
7320actctgattg acaagtccaa gtctgtcaag tctgtcttga tgcaattgtt
tggtgaaaac 7380actgacgtcg aaggtattga cacgcttaat gcctgttacg
gtggtaccaa cgcgttgttc 7440aactctttga actggattga atctaacgca
tgggatggta gagacgccat tgtagtttgc 7500ggtgatattg ccatctacga
taagggtgcc gcaagaccaa ccggtggtgc cggtactgtt 7560gctatgtgga
tcggtcctga tgctccaatt gtatttgact ctgtaagagc ttcttacatg
7620gaacacgcct acgattttta caagccagat ttcaccagcg aatatcctta
cgtcgatggt 7680catttttcat taacttgtta cgtcaaggct cttgatcaag
tttacaagag ttattccaag 7740aaggctattt ctaaagggtt ggttagcgat
cccgctggtt cggatgcttt gaacgttttg 7800aaatatttcg actacaacgt
tttccatgtt ccaacctgta aattggtcac aaaatcatac 7860ggtagattac
tatataacga tttcagagcc aatcctcaat tgttcccaga agttgacgcc
7920gaattagcta ctcgcgatta tgacgaatct ttaaccgata agaacattga
aaaaactttt 7980gttaatgttg ctaagccatt ccacaaagag agagttgccc
aatctttgat tgttccaaca 8040aacacaggta acatgtacac cgcatctgtt
tatgccgcct ttgcatctct attaaactat 8100gttggatctg acgacttaca
aggcaagcgt gttggtttat tttcttacgg ttccggttta 8160gctgcatctc
tatattcttg caaaattgtt ggtgacgtcc aacatattat caaggaatta
8220gatattacta acaaattagc caagagaatc accgaaactc caaaggatta
cgaagctgcc 8280atcgaattga gagaaaatgc ccatttgaag aagaacttca
aacctcaagg ttccattgag 8340catttgcaaa gtggtgttta ctacttgacc
aacatcgatg acaaatttag aagatcttac 8400gatgttaaaa aataatcttc
ccccatcgat tgcatcttgc tgaaccccct tcataaatgc 8460tttatttttt
tggcagcctg ctttttttag ctctcattta atagagtagt tttttaatct
8520atatactagg aaaactcttt atttaataac aatgatatat atatattcca
gtggtgcatg 8580aacgcatgag aaagcccccg gaagatcatc ttccgggggc
tttttttttg gcgcgcgata 8640cagaccggtt cagacaggat aaagaggaac
gcagaatgtt agacaacacc cgcttacgca 8700tagctattca gaaatcaggc
cgtttaagcg atgattcacg agaattgctg gcccgctgcg 8760gcataaaaat
taatttacac actcagcgcc tgattgcgat ggcggaaaac atgccgattg
8820atatcctgcg cgtgcgtgat gatgacattc cgggtctggt aatggatggc
gtggtcgatc 8880tcggtattat cggcgaaaac gtgctggaag aagagctact
caaccgccgc gcacagggcg 8940aagatccacg ctatttaacc ctgcgccgtc
ttgacttcgg cggctgccgt ttatcgctgg 9000caacaccggt tgacgaagcc
tgggacggcc cggccgcgct ggacggtaaa cgtatcgcta 9060cctcatatcc
gcacctcctc aaacgctacc tcgaccagaa aggcgtctct tttaaatcgt
9120gtctgttaaa tggttctgtc gaagtcgcgc cgcgcgcggg gctggccgac
gctatctgcg 9180atttggtctc taccggcgcg acgcttgaag ctaacggcct
gcgtgaagtc gaagttatct 9240accgctctaa agcctgtctg attcagcgcg
acggtgagat ggcacagagc aagcaagagc 9300tgatcgataa attgctgacc
cgtattcagg gcgtgattca ggcgcgcgaa tcgaaataca 9360tcatgatgca
cgcgccaagt gaacgcctgg aagaggttat cgccctgctg ccaggcgccg
9420aaaggccgac aattctgccg ctggcaggcg agcaacagcg cgtggcgatg
cacatggtca 9480gcagcgaaac gttgttctgg gaaaccatgg agaaactgaa
agcgcttggc gccagctcga 9540ttctggtact gccgatcgag aagatgatgg
agtgatctga cgcctgatgg cgctgcgctt 9600atcaggccta cgtaatgcgt
tgaaaaactg tattataagt aaatgcatgt atactaaact 9660cacaaattag
agcttcaatt taattatatc agttattacc cgggaatctc ggtcgtaatg
9720atttctataa tgacgaaaaa aaaaaaattg gaaagaaaaa gcttcatggc
ctttataaaa 9780aggaactatc caatacctcg ccagaaccaa gtaacagtat
tttacggggc acaaatcaag 9840aacaataaga caggactgta aagatggacg
cattgaactc caaagaacaa caagagttcc 9900aaaaagtagt ggaacaaaag
caaatgaagg atttcatgcg tttgtactct aatctggtag 9960aaagatgttt
cacagactgt gtcaatgact tcacaacatc aaagctaacc aataaggaac
10020aaacatgcat catgaagtgc tcagaaaagt tcttgaagca tagcgaacgt
gtagggcagc 10080gtttccaaga acaaaacgct gccttgggac aaggcttggg
ccgataaggt gtactggcgt 10140atatatatct aattatgtat ctctggtgta
gcccattttt agcatgtaaa tataaagaga 10200aaccatatct aatctaacca
aatccaaaca aaattcaata gttactatcg cttttttctt 10260tctgtatcgc
aaataagtga aaattaaaaa agaaagatta aattggaagt tggatatggg
10320ctggaacagc agcagtaatc ggtatcgggt tcgccactaa tgacgtccta
cgattgcact 10380caacagacct tgacgctcac gccgtagcgg gcgacaagtc
aaacggaaca accgttgccg 10440ttcccatcgg agtccgacct aggccgaact
ccgtgaattt ctgataacaa cggtcggtaa 10500agactggttc cccagtatat
ttcttctctc aggagcaggg gccaatgcca aaagcgacat 10560taacccggag
gacaaggctc cactgtgttc caccgaattt cccacctgat aatatctgat 10620aac
10623248479DNAArtificial SequenceSynthetic Integration construct
i467 24gacggcacgg ccacgcgttt aaaccgccct ccaagctgac ataaatcgca
ctttgtatct 60actttttttt attcgaaaac aaggcacaac aatgaatcta tcgccctgtg
agattttcaa 120tctcaagttt gtgtaataga tagcgttata ttatagaact
ataaaggtcc ttgaatatac 180atagtgtttc attcctatta ctgtatatgt
gactttacat tgttacttcc gcggctattt 240gacgttttct gcttcaggtg
cggcttggag ggcaaagtgt cagaaaatcg gccaggccgt 300atgacacaaa
agagtagaaa acgagatctc aaatatctcg aggcctgtcc tctatacaac
360cgcccagctc tctgacaaag ctccagaacg gttgtctttt gtttcgaaaa
gccaaggtcc 420cttataattg ccctccattt tgtgtcacct atttaagcaa
aaaattgaaa gtttactaac 480ctttcattaa agagaaataa caatattata
aaaagcgctt aaagctcaca cgcggccagg 540gggagccgtt catcatctca
tggatctgca catgaacaaa caccagagtc aaacgacgtt 600gaaattgagg
ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta
660tctgatgtag aaaaggatta aagatgctaa gagatagtga tgatatttca
taaataatgt 720aattctatat atgttaatta ccttttttgc gaggcatatt
tatggtgaag gataagtttt 780gaccatcaaa gaaggttaat gtggctgtgg
tttcagggtc cataaagctt ttcaattcat 840cttttttttt tttgttcttt
tttttgattc cggtttcttt gaaatttttt tgattcggta 900atctccgagc
agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc
960atatgtggtg ttgaagaaac atgaaattgc ccagtattct taacccaact
gcacagaaca 1020aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta
catataagga acgtgctgct 1080actcatccta gtcctgttgc tgccaagcta
tttaatatca tgcacgaaaa gcaaacaaac 1140ttgtgtgctt cattggatgt
tcgtaccacc aaggaattac tggagttagt tgaagcatta 1200ggtcccaaaa
tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag
1260ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact
cttcgaagac 1320agaaaatttg ctgacattgg taatacagtc aaattgcagt
actctgcggg tgtatacaga 1380atagcagaat gggcagacat tacgaatgca
cacggtgtgg tgggcccagg tattgttagc 1440ggtttgaagc aggcggcaga
agaagtaaca aaggaaccta gaggcctttt gatgttagca 1500gaattgtcat
gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt
1560gcgaagagtg acaaagattt tgttatcggc tttattgctc aaagagacat
gggtggaaga 1620gatgaaggtt acgattggtt gattatgaca cccggtgtgg
gtttagatga caagggagac 1680gcattgggtc aacagtatag aaccgtggat
gatgtggtct ctacaggatc tgacattatt 1740attgttggaa gaggactatt
tgcaaaggga agggatgcta aggtagaggg tgaacgttac 1800agaaaagcag
gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta
1860ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta
attatatcag 1920ttattacccg ggaatctcgg tcgtaatgat ttctataatg
acgaaaaaaa aaaaattgga 1980aagaaaaagc ttcatggcct ttataaaaag
gaactatcca atacctcgcc agaaccaagt 2040aacagtattt tacggggcac
aaatcaagaa caataagaca ggactgtaaa gatggacgca 2100tcgctcgtcc
aacgccggcg gacctgtttt caatagttcg gtaatattaa cggataccta
2160ctattatccc ctagtaggct cttttcacgg agaaattcgg gagtgttttt
tttccgtgcg 2220cattttctta gctatattct tccagcttcg cctgctgccc
ggtcatcgtt cctgtcacgt 2280agtttttccg gattcgtccg gctcatataa
taccgcaata aacacggaat atctcgttcc 2340gcggattcgg ttaaactctc
ggtcgcggat tatcacagag aaagcttcgt ggagaatttt 2400tccagatttt
ccgctttccc cgatgttggt atttccggag gtcattatac tgaccgccat
2460tataatgact gtacaacgac cttctggaga aagaaacaac tcaataacga
tgtgggacat 2520tgggggccca ctcaaaaaat ctggggacta tatccccaga
gaatttctcc agaagagaag 2580aaaagtcaaa gttttttttc gcttgggggt
tgcatataaa tacaggcgct gttttatctt 2640cagcatgaat attccataat
tttacttaat agcttttcat aaataataga atcacaaaca 2700aaatttacat
ctgagttaaa caatcatgac aatcaaggaa cataaagtag tttatgaagc
2760tcacaacgta aaggctctta aggctcctca acatttttac aacagccaac
ccggcaaggg 2820ttacgttact gatatgcaac attatcaaga aatgtatcaa
caatctatca atgagccaga 2880aaaattcttt gataagatgg ctaaggaata
cttgcattgg gatgctccat acaccaaagt 2940tcaatctggt tcattgaaca
atggtgatgt tgcatggttt ttgaacggta aattgaatgc 3000atcatacaat
tgtgttgaca gacatgcctt tgctaatccc gacaagccag ctttgatcta
3060tgaagctgat gacgaatccg acaacaaaat catcacattt ggtgaattac
tcagaaaagt 3120ttcccaaatc gctggtgtct taaaaagctg gggcgttaag
aaaggtgaca cagtggctat 3180ctatttgcca atgattccag aagcggtcat
tgctatgttg gctgtggctc gtattggtgc 3240tattcactct gttgtctttg
ctgggttctc cgctggttcg ttgaaagatc gtgtcgttga 3300cgctaattct
aaagtggtca tcacttgtga tgaaggtaaa agaggtggta agaccatcaa
3360cactaaaaaa attgttgacg aaggtttgaa cggagtcgat ttggtttccc
gtatcttggt 3420tttccaaaga actggtactg aaggtattcc aatgaaggcc
ggtagagatt actggtggca 3480tgaggaggcc gctaagcaga gaacttacct
acctcctgtt tcatgtgacg ctgaagatcc 3540tctattttta ttatacactt
ccggttccac tggttctcca aagggtgtcg ttcacactac 3600aggtggttat
ttattaggtg ccgctttaac aactagatac gtttttgata ttcacccaga
3660agatgttctc ttcactgccg gtgacgtcgg ctggatcacg ggtcacacct
atgctctata 3720tggtccatta accttgggta ccgcctcaat aattttcgaa
tccactcctg cctacccaga 3780ttatggtaga tattggagaa ttatccaacg
tcacaaggct acccatttct atgtggctcc 3840aactgcttta agattaatca
aacgtgtagg tgaagccgaa attgccaaat atgacacttc 3900ctcattacgt
gtcttgggtt ccgtcggtga accaatctct ccagacttat gggaatggta
3960tcatgaaaaa gtgggtaaca aaaactgtgt catttgtgac actatgtggc
aaacagagtc 4020tggttctcat ttaattgctc ctttggcagg tgctgtccca
acaaaacctg gttctgctac 4080cgtgccattc tttggtatta acgcttgtat
cattgaccct gttacaggtg tggaattaga 4140aggtaatgat gtcgaaggtg
tccttgccgt taaatcacca tggccatcaa tggctagatc 4200tgtttggaac
caccacgacc gttacatgga tacttacttg aaaccttatc ctggtcacta
4260tttcacaggt gatggtgctg gtagagatca tgatggttac tactggatca
ggggtagagt 4320tgacgacgtt gtaaatgttt ccggtcatag attatccaca
tcagaaattg aagcatctat 4380ctcaaatcac gaaaacgtct cggaagctgc
tgttgtcggt attccagatg aattgaccgg 4440tcaaaccgtc gttgcatatg
tttccctaaa agatggttat ctacaaaaca acgctactga 4500aggtgatgca
gaacacatca caccagataa tttacgtaga gaattgatct tacaagttag
4560gggtgagatt ggtcctttcg cctcaccaaa aaccattatt ctagttagag
atctaccaag 4620aacaaggtca ggaaagatta
tgagaagagt tctaagaaag gttgcttcta acgaagccga 4680acagctaggt
gacctaacta ctttggccaa cccagaagtt gtacctgcca tcatttctgc
4740tgtagagaac caatttttct ctcaaaaaaa gaaataaatt gaattgaatt
gaaatcgata 4800gatcaatttt tttcttttct ctttccccat cctttacgct
aaaataatag tttattttat 4860tttttgaata ttttttattt atatacgtat
atatagacta ttatttatct tttaatgatt 4920attaagattt ttattaaaaa
aaaattcgct cctcttttaa tgcctttatg cagttttttt 4980ttcccattcg
atatttctat gttcgggttc agcgtatttt aagtttaata actcgaaaat
5040tctgcgttcg ttaaagcttt cgagaaggat attatttcga aataaaccgt
gttgtgtaag 5100cttgaagcct ttttgcgctg ccaatattct tatccatcta
ttgtactctt tagatccagt 5160atagtgtatt cttcctgctc caagctcatc
ccatccccgc gtgcttggcc ggccgttttg 5220ccagcttact atccttcttg
aaaatatgca ctctatatct tttagttctt aattgcaaca 5280catagatttg
ctgtataacg aattttatgc tattttttaa atttggagtt cagtgataaa
5340agtgtcacag cgaatttcct cacatgtagg gaccgaattg tttacaagtt
ctctgtacca 5400ccatggagac atcaaaaatt gaaaatctat ggaaagatat
ggacggtagc aacaagaata 5460tagcacgagc cgcggagttc atttcgttac
ttttgatatc actcacaact attgcgaagc 5520gcttcagtga aaaaatcata
aggaaaagtt gtaaatatta ttggtagtat tcgtttggta 5580aagtagaggg
ggtaattttt cccctttatt ttgttcatac attcttaaat tgctttgcct
5640ctccttttgg aaagctatac ttcggagcac tgttgagcga aggctcatta
gatatatttt 5700ctgtcatttt ccttaaccca aaaataaggg aaagggtcca
aaaagcgctc ggacaactgt 5760tgaccgtgat ccgaaggact ggctatacag
tgttcacaaa atagccaagc tgaaaataat 5820gtgtagctat gttcagttag
tttggctagc aaagatataa aagcaggtcg gaaatattta 5880tgggcattat
tatgcagagc atcaacatga taaaaaaaaa cagttgaata ttccctcaaa
5940aatgtcttac accgtcggaa cctacttggc cgagaggttg gtccagatcg
gattgaagca 6000ccacttcgcc gtcgccggtg actacaactt ggtcttgttg
gacaacttgt tgttgaacaa 6060gaacatggag caggtctatt gctgcaacga
gttgaactgc ggtttctcag cagaaggtta 6120tgcaagagcc aagggagcag
ccgctgccgt cgtcacctac tcagtcggtg cattatcagc 6180attcgatgca
attggaggtg cttacgctga gaacttgcca gtcatcttga tctctggagc
6240acctaacaac aacgaccatg ctgctggtca cgtattgcac cacgccttgg
gtaaaacaga 6300ctaccactac cagttggaaa tggcaaaaaa tattaccgca
gccgcagagg ccatctacac 6360cccagaggaa gcacctgcca aaattgacca
cgtcataaag accgctttga gagagaagaa 6420gcctgtttac ttggagatcg
cctgcaacat cgcttctatg ccatgcgccg cacctggtcc 6480agcctctgct
ttgttcaacg acgaggcctc tgacgaagct tcattgaacg ccgcagtcga
6540agagacatta aagttcatcg ccaacaggga caaagttgcc gtcttagtcg
gttcaaagtt 6600gagggccgct ggtgccgaag aggcagctgt caagttcgct
gacgccttgg gaggagccgt 6660cgccaccatg gccgcagcaa aatctttctt
tcctgaggag aacccacatt acatcggaac 6720ctcatggggt gaagtatcat
atcctggagt agaaaaaacc atgaaagagg ccgatgccgt 6780aatagcattg
gctcctgtct tcaacgacta ctcaaccaca ggatggactg atataccaga
6840tccaaagaaa ttagtcttgg ctgagcctag gtctgtcgtc gtaaacggta
tcaggttccc 6900ttctgttcat ttgaaggact acttaacaag attggcccaa
aaggtatcta aaaagactgg 6960tgccttggac ttcttcaagt cattaaacgc
aggagaattg aaaaaagcag caccagccga 7020tccatcagcc ccattagtta
acgctgaaat cgctagacaa gtagaggctt tgttgactcc 7080aaacactacc
gtcatagctg agacaggtga ctcttggttc aacgcacaga gaatgaaatt
7140gccaaatggt gccagggtcg agtatgaaat gcagtgggga catataggtt
ggtcagtccc 7200agccgccttt ggatacgcag taggtgcccc tgagaggagg
aacatattga tggttggtga 7260tggttcattc caattaacag cccaggaggt
agcccaaatg gtcaggttga agttgcctgt 7320catcatcttc ttgatcaaca
attacggata caccatcgag gtcatgatcc acgacggacc 7380ttacaacaac
atcaaaaact gggactacgc cggtttgatg gaggttttca acggtaacgg
7440tggttatgac tcaggagccg gtaagggatt aaaggctaag accggtggtg
aattggctga 7500agcaattaag gtcgcattgg ccaacaccga tggacctaca
ttgattgaat gcttcatcgg 7560aagggaggac tgcaccgagg aattggttaa
atggggtaaa agggtagccg ctgctaattc 7620aagaaaacca gttaataaat
tattataata agtgaattta ctttaaatct tgcatttaaa 7680taaattttct
ttttatagct ttatgactta gtttcaattt atatactatt ttaatgacat
7740tttcgattca ttgattgaaa gctttgtgtt ttttcttgat gcgctattgc
attgttcttg 7800tctttttcgc cacatgtaat atctgtagta gatacctgat
acattgtgga tgctgagtga 7860aattttagtt aataatggag gcgctcttaa
taattttggg gatattggct taacctgcag 7920gccgcgagcg ccgatataaa
ctaatgattt taaatcgtta aaaaaatatg cgaattctgt 7980ggatcgaaca
caggacctcc agataacttg accgaagttt tttcttcagt ctggcgctct
8040cccaactgag ctaaatccgc ttactatttg ttatcagttc ccttcatatc
tacatagaat 8100aggttaagta ttttattagt tgccagaaga actactgata
gttgggaata tttggtgaat 8160aatgaagatt gggtgaataa tttgataatt
ttgagattca attgttaatc aatgttacaa 8220tattatgtat acagagtata
ctagaagttc tcttcggaga tcttgaagtt cacaaaaggg 8280aatcgatatt
tctacataat attatcatta cttcttcccc atcttatatt tgtcattcat
8340tattgattat gatcaatgca ataatgattg gtagttgcca aacatttaat
acgatcctct 8400gtaatatttc tatgaataat tatcacagca acgttcaatt
atcttcaatt ccggtgttta 8460aaccccagcg cctggcggg
8479255628DNAArtificial SequenceSynthetic URA3 FcphI target site
cassette 25gcctgtctac aggataaaga cgggtcggat acctgcacaa gcaatttggc
acctgcatac 60cccatttccc cagtagataa cttcaacaca cacatcaatg tccctcacca
gtttatttcc 120aaaagagacg ctttttacta cctgactaga ttttcatttt
gtttcttttg gattgcgctt 180gcctttgtag gtgtgtcgtt tatcctttac
gttttgactt ggtgctcgaa gatgctttca 240gagatggtgc ttatcctcat
gtcttttggg tttgtcttca atacggcagc cgttgtcttg 300caaacggccg
cctctgccat ggcaaagaat gctttccatg acgatcatcg tagtgcccaa
360ttgggtgcct ctatgatggg tttaaacgta tggcttgggc aagtgtcttt
ttatgtatcg 420tggaatttat cctgctggtc ttctggtctg ttagggcaag
gttggcctct acttactcca 480tcgacaattc aagatacaga acctcctcca
gatggaatcc cttccataga gagaaggagc 540aagcaactga cccaatattg
actgccactg gacctgaaga catgcaacaa agtgcaagca 600tagtggggcc
ttcttccaat gctaatccgg tcactgccac tgctgctacg gaaaaccaac
660ctaaaggtat taacttcttc actataagaa aatcacacga gcgcccggac
gatgtctctg 720tttaaatggc gcaagttttc cgctttgtaa tatatattta
tacccctttc ttctctcccc 780tgcaatataa tagtttaatt ctaatattaa
taatatccta tattttcttc atttaccggc 840gcactctcgc ccgaacgacc
tcaaaatgtc tgctacattc ataataacca aaagctcata 900actttttttt
ttgaacctga atatatatac atcacatatc actgctggtc ctttgtgagc
960gttgcgctcg tgcatcatgc gtccatcttt acagtcctgt cttattgttc
ttgatttgtg 1020ccccgtaaaa tactgttact tggttctggc gaggtattgg
atagttcctt tttataaagg 1080ccatgaagct ttttctttcc aatttttttt
ttttcgtcat tatagaaatc attacgaccg 1140agattcccgg gtaataactg
atataattaa attgaagctc taatttgtga gtttagtata 1200catgcattta
cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
1260tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
ctttgcaaat 1320agtcctcttc caacaataat aatgtcagat cctgtagaga
ccacatcatc cacggttcta 1380tactgttgac ccaatgcgtc tcccttgtca
tctaaaccca caccgggtgt cataatcaac 1440caatcgtaac cttcatctct
tccacccatg tctctttgag caataaagcc gataacaaaa 1500tctttgtcac
tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
1560cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt
tacttcttct 1620gccgcctgct tcaaaccgct aacaatacct gggcccacca
caccgtgtgc attcgtaatg 1680tctgcccatt ctgctattct gtatacaccc
gcagagtact gcaatttgac tgtattacca 1740atgtcagcaa attttctgtc
ttcgaagagt aaaaaattgt acttggcgga taatgccttt 1800agcggcttaa
ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
1860aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt
ggtacgaaca 1920tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
tattaaatag cttggcagca 1980acaggactag gatgagtagc agcacgttcc
ttatatgtag ctttcgacat gatttatctt 2040cgtttcctgc aggtttttgt
tctgtgcagt tgggttaaga atactgggca atttcatgtt 2100tcttcaacac
cacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
2160tccttctgct cggagattac cgaatcaaaa aaatttcaaa gaaaccggaa
tcaaaaaaaa 2220gaacaaaaaa aaaaaagatg aattgaaaag ctttatggac
cctgaaacca cagccacatt 2280aaccttcttt gatggtcaaa acttatcctt
caccataaat atgcctcgca aaaaaggtaa 2340ttaacatata tagaattaca
ttatttatga aatatcatca ctatctctta gcatctttaa 2400tccttttcta
catcagataa cttcggtttg ttatcatcgt ctgtattgtc atcaattggc
2460gcagtagcct caatttcaac gtcgtttgac tctggtgttt gttcatgtgc
agatccatga 2520gatgatgaac ttgtgagcgt tgcgctcgtg catcaccata
tacatatcca tatctaatct 2580tacttatatg ttgtggaaat gtaaagagcc
ccattatctt agcctaaaaa aaccttctct 2640ttggaacttt cagtaatacg
cttaactgct cattgctata ttgaagtacg gattagaagc 2700cgccgagcgg
gcgacagccc tccgacggaa gactctcctc cgtgcgtcct ggtcttcacc
2760ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
ataaagattc 2820tacaatacta gcttttatgg ttatgaagag gaaaaattgg
cagtaacctg gccccacaaa 2880ccttcaaatc aacgaatcaa attaacaacc
ataggataat aatgcgatta gttttttagc 2940cttatttctg gggtaattaa
tcagcgaagc gatgattttt gatctattaa cagatatata 3000aatgcaaaag
ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
3060tcttattcaa atgtcataaa agtatcaaca aaaaattgtt aatatacctc
tatactttaa 3120cgtcaaggag aaaaaactat aatgtcatta ccgttcttaa
cttctgcacc gggaaaggtt 3180attatttttg gtgaacactc tgctgtgtac
aacaagcctg ccgtcgctgc tagtgtgtct 3240gcgttgagaa cctacctgct
aataagcgag tcatctgcac cagatactat tgaattggac 3300ttcccggaca
ttagctttaa tcataagtgg tccatcaatg atttcaatgc catcaccgag
3360gatcaagtaa actcccaaaa attggccaag gctcaacaag ccaccgatgg
cttgtctcag 3420gaactcgtta gtcttttgga tccgttgtta gctcaactat
ccgaatcctt ccactaccat 3480gcagcgtttt gtttcctgta tatgtttgtt
tgcctatgcc cccatgccaa gaatattaag 3540ttttctttaa agtctacttt
acccatcggt gctgggttgg gctcaagcgc ctctatttct 3600gtatcactgg
ccttagctat ggcctacttg ggggggttaa taggatctaa tgacttggaa
3660aagctgtcag aaaacgataa gcatatagtg aatcaatggg ccttcatagg
tgaaaagtgt 3720attcacggta ccccttcagg aatagataac gctgtggcca
cttatggtaa tgccctgcta 3780tttgaaaaag actcacataa tggaacaata
aacacaaaca attttaagtt cttagatgat 3840ttcccagcca ttccaatgat
cctaacctat actagaattc caaggtctac aaaagatctt 3900gttgctcgcg
ttcgtgtgtt ggtcaccgag aaatttcctg aagttatgaa gccaattcta
3960gatgccatgg gtgaatgtgc cctacaaggc ttagagatca tgactaagtt
aagtaaatgt 4020aaaggcaccg atgacgaggc tgtagaaact aataatgaac
tgtatgaaca actattggaa 4080ttgataagaa taaatcatgg actgcttgtc
tcaatcggtg tttctcatcc tggattagaa 4140cttattaaaa atctgagcga
tgatttgaga attggctcca caaaacttac cggtgctggt 4200ggcggcggtt
gctctttgac tttgttacga agagacatta ctcaagagca aattgacagt
4260ttcaaaaaga aattgcaaga tgattttagt tacgagacat ttgaaacaga
cttgggtggg 4320actggctgct gtttgttaag cgcaaaaaat ttgaataaag
atcttaaaat caaatcccta 4380gtattccaat tatttgaaaa taaaactacc
acaaagcaac aaattgacga tctattattg 4440ccaggaaaca cgaatttacc
atggacttca taagctaatt tgcgataggc attatttatt 4500agttgttttt
aatcttaact gtgtatgaag ttttatgtaa taaagataga aagagaaaca
4560aaaaaaaatt tttcgtagta tcaattcagc tttcgaagac agaatgaaat
ttaagcagac 4620catcatcttg ccctgtgctt ggcccccagt gcagcgaacg
ttataaaaac gaatactgag 4680tatatatcta tgtaaaacaa ccatatcatt
tcttgttctg aactttgttt acctaactag 4740ttttaaattt ccctttttcg
tgcatgcggg tgttcttatt tattagcata ctacatttga 4800aatatcaaat
ttccttagta gaaaagtgag agaaggtgca ctgacacaaa aaataaaatg
4860ctacgtataa ctgtcaaaac tttgcagcag cgggcatcct tccatcatag
cttcaaacat 4920attagcgttc ctgatcttca tacccgtgct caaaatgatc
aaacaaactg ttattgccaa 4980gaaataaacg caaggctgcc ttcaaaaact
gatccattag atcctcatat caagcttcct 5040catagaacgc ccaattacaa
taagcatgtt ttgctgttat caccgggtga taggtttgct 5100caaccatgga
aggtagcatg gaatcataat ttggatacta atacaaatcg gccatataat
5160gccattagta aattgcgctc ccatttaggt ggttctccag gcaaatttga
atactaataa 5220atgcggtgca tttgcaaaat gaatttattc caaggccaaa
acaacacgat gaatggcttt 5280atttttttgt tattcctgac atgaagcttt
atgtaattaa ggaaacggac atcgaggaat 5340ttgcatcttt tttagatgaa
ggagctattc aagcaccaaa gctatccttc caggattatt 5400taagcggtaa
ggccaaggct tcccaacagg ttcatgaagt gcatcataga aagcttacaa
5460ggtttcaggg tgaaactttt ctaagagatt ggaacttagt ctgtgggcat
tataagagag 5520atgctaagtg tggagaaatg ggacccgaca taattgcagc
atttcaagat gaaaagcttt 5580ttcctgagaa taatctagcc ttaatttctc
atattggggg tcatattt 56282610019DNAArtificial SequenceSynthetic HXT3
FcphI target site cassette 26accggtatat caaatggcgg tgtagtttga
aaagtacact gtatgtccat taataaaatt 60acgccgaaga acactgacta gctataaatt
ctcttccggc gtccaatttc aacctaaggc 120aagtattgtt aatcaataat
gcgtgttggt aaatatgaag cccgatgttg attaagaaga 180attattcgta
taagaattaa gcaagcaaaa ttaaggaaaa ttttttcttt cctattcgtc
240atcgcagaca gccttcatct tctcgagata acacctggag gaggagcaat
gaaatgaaag 300gaaaaaaaaa tactttcttt ttcttgaaaa aagaaaaaaa
ttgtaagatg agctattcgc 360ggaacattct agctcgtttg catcttcttg
catttggtag gttttcaata gttcggtaat 420attaacggat acctactatt
atcccctagt aggctctttt cacggagaaa ttcgggagtg 480ttttttttcc
gtgcgcattt tcttagctat attcttccag cttcgcctgc tgcccggtca
540tcgttcctgt cacgtagttt ttccggattc gtccggctca tataataccg
caataaacac 600ggaatatctc gttccgcgga ttcggttaaa ctctcggtcg
cggattatca cagagaaagc 660ttcgtggaga atttttccag attttccgct
ttccccgatg ttggtatttc cggaggtcat 720tatactgacc gccattataa
tgactgtaca acgaccttct ggagaaagaa acaactcaat 780aacgatgtgg
gacattgggg gcccactcaa aaaatctggg gactatatcc ccagagaatt
840tctccagaag agaagaaaag tcaaagtttt ttttcgcttg ggggttgcat
ataaattgtg 900agcgttgcgc tcgtgcatcg tcgacactag taatacacat
catcgtccta caagttcatc 960aaagtgttgg acagacaact ataccagcat
ggatctcttg tatcggttct tttctcccgc 1020tctctcgcaa taacaatgaa
cactgggtca atcatagcct acacaggtga acagagtagc 1080gtttatacag
ggtttatacg gtgattccta cggcaaaaat ttttcatttc taaaaaaaaa
1140aagaaaaatt tttctttcca acgctagaag gaaaagaaaa atctaattaa
attgatttgg 1200tgattttctg agagttccct ttttcatata tcgaattttg
aatataaaag gagatcgaaa 1260aaatttttct attcaatctg ttttctggtt
ttatttgata gtttttttgt gtattattat 1320tatggattag tactggttta
tatgggtttt tctgtataac ttctttttat tttagtttgt 1380ttaatcttat
tttgagttac attatagttc cctaactgca agagaagtaa cattaaaaat
1440gaccactctt gacgacacgg cttaccggta ccgcaccagt gtcccggggg
acgccgaggc 1500catcgaggca ctggatgggt ccttcaccac cgacaccgtc
ttccgcgtca ccgccaccgg 1560ggacggcttc accctgcggg aggtgccggt
ggacccgccc ctgaccaagg tgttccccga 1620cgacgaatcg gacgacgaat
cggacgccgg ggaggacggc gacccggact cccggacgtt 1680cgtcgcgtac
ggggacgacg gcgacctggc gggcttcgtg gtcgtctcgt actccggctg
1740gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg gagcaccggg
ggcacggggt 1800cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc
gagcggggcg ccgggcacct 1860ctggctggag gtcaccaacg tcaacgcacc
ggcgatccac gcgtaccggc ggatggggtt 1920caccctctgc ggcctggaca
ccgccctgta cgacggcacc gcctcggacg gcgagcaggc 1980gctctacatg
agcatgccct gcccctgagt ttaacttgat actactagat tttttctctt
2040catttataaa atttttggtt ataattgaag ctttagaagt atgaaaaaat
cctttttttt 2100cattctttgc aaccaaaata agaagcttct tttattcatt
gaaatgatga atataaacct 2160aacaaaagaa aaagactcga atatcaaaca
ttaaaaaaaa ataaaagagg ttatctgttt 2220tcccatttag ttggagtttg
cattttctaa tagatagaac tctcaattaa tgtggattta 2280gtttctctgt
tcgttttttt ttgttttgtt ctcactgtat ttacatttct atttagtatt
2340tagttattca tataatctta acttctcgag gagctcgatg cgtccatctt
tacagtcctg 2400tcttattgtt cttgatttgt gccccgtaaa atactgttac
ttggttctgg cgaggtattg 2460gatagttcct ttttataaag gccatgaagc
tttttctttc caattttttt tttttcgtca 2520ttatagaaat cattacgacc
gagattcccg ggtaataact gatataatta aattgaagct 2580ctaatttgtg
agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
2640ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
ctctacctta 2700gcatcccttc cctttgcaaa tagtcctctt ccaacaataa
taatgtcaga tcctgtagag 2760accacatcat ccacggttct atactgttga
cccaatgcgt ctcccttgtc atctaaaccc 2820acaccgggtg tcataatcaa
ccaatcgtaa ccttcatctc ttccacccat gtctctttga 2880gcaataaagc
cgataacaaa atctttgtca ctcttcgcaa tgtcaacagt acccttagta
2940tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
aaggcctcta 3000ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc
taacaatacc tgggcccacc 3060acaccgtgtg cattcgtaat gtctgcccat
tctgctattc tgtatacacc cgcagagtac 3120tgcaatttga ctgtattacc
aatgtcagca aattttctgt cttcgaagag taaaaaattg 3180tacttggcgg
ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
3240atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
taactccagt 3300aattccttgg tggtacgaac atccaatgaa gcacacaagt
ttgtttgctt ttcgtgcatg 3360atattaaata gcttggcagc aacaggacta
ggatgagtag cagcacgttc cttatatgta 3420gctttcgaca tgatttatct
tcgtttcctg caggtttttg ttctgtgcag ttgggttaag 3480aatactgggc
aatttcatgt ttcttcaaca ccacatatgc gtatatatac caatctaagt
3540ctgtgctcct tccttcgttc ttccttctgc tcggagatta ccgaatcaaa
aaaatttcaa 3600agaaaccgga atcaaaaaaa agaacaaaaa aaaaaaagat
gaattgaaaa gctttatgga 3660ccctgaaacc acagccacat taaccttctt
tgatggtcaa aacttatcct tcaccataaa 3720tatgcctcgc aaaaaaggta
attaacatat atagaattac attatttatg aaatatcatc 3780actatctctt
agcatcttta atccttttct acatcagata acttcggttt gttatcatcg
3840tctgtattgt catcaattgg cgcagtagcc tcaatttcaa cgtcgtttga
ctctggtgtt 3900tgttcatgtg cagatccatg agatgatgaa cttgtgagcg
ttgcgctcgt gcatccgctc 3960gtccaacgcc ggcggacctc gctcgtccaa
cgccggcgga cctcttttaa ttctgctgta 4020acccgtacat gcccaaaata
gggggcgggt tacacagaat atataacatc gtaggtgtct 4080gggtgaacag
tttattcctg gcatccacta aatataatgg agcccgcttt ttaagctggc
4140atccagaaaa aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa
ccatcagttc 4200ataggtccat tctcttagcg caactacaga gaacaggggc
acaaacaggc aaaaaacggg 4260cacaacctca atggagtgat gcaacctgcc
tggagtaaat gatgacacaa ggcaattgac 4320ccacgcatgt atctatctca
ttttcttaca ccttctatta ccttctgctc tctctgattt 4380ggaaaaagct
gaaaaaaaag gttgaaacca gttccctgaa attattcccc tacttgacta
4440ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt
cttaaacttc 4500ttaaattcta cttttatagt tagtcttttt tttagtttta
aaacaccaag aacttagttt 4560cgatccccgc gtgcttggcc ggccgtatcc
ccgcgtgctt ggccggccgt atgtctcaga 4620acgtttacat tgtatcgact
gccagaaccc caattggttc attccagggt tctctatcct 4680ccaagacagc
agtggaattg ggtgctgttg ctttaaaagg cgccttggct aaggttccag
4740aattggatgc atccaaggat tttgacgaaa ttatttttgg taacgttctt
tctgccaatt 4800tgggccaagc tccggccaga caagttgctt tggctgccgg
tttgagtaat catatcgttg 4860caagcacagt taacaaggtc tgtgcatccg
ctatgaaggc aatcattttg ggtgctcaat 4920ccatcaaatg tggtaatgct
gatgttgtcg tagctggtgg ttgtgaatct atgactaacg 4980caccatacta
catgccagca gcccgtgcgg gtgccaaatt tggccaaact gttcttgttg
5040atggtgtcga aagagatggg ttgaacgatg cgtacgatgg tctagccatg
ggtgtacacg 5100cagaaaagtg tgcccgtgat tgggatatta ctagagaaca
acaagacaat tttgccatcg 5160aatcctacca aaaatctcaa aaatctcaaa
aggaaggtaa attcgacaat gaaattgtac 5220ctgttaccat taagggattt
agaggtaagc ctgatactca agtcacgaag gacgaggaac 5280ctgctagatt
acacgttgaa aaattgagat ctgcaaggac tgttttccaa aaagaaaacg
5340gtactgttac tgccgctaac gcttctccaa tcaacgatgg tgctgcagcc
gtcatcttgg 5400tttccgaaaa agttttgaag
gaaaagaatt tgaagccttt ggctattatc aaaggttggg 5460gtgaggccgc
tcatcaacca gctgatttta catgggctcc atctcttgca gttccaaagg
5520ctttgaaaca tgctggcatc gaagacatca attctgttga ttactttgaa
ttcaatgaag 5580ccttttcggt tgtcggtttg gtgaacacta agattttgaa
gctagaccca tctaaggtta 5640atgtatatgg tggtgctgtt gctctaggtc
acccattggg ttgttctggt gctagagtgg 5700ttgttacact gctatccatc
ttacagcaag aaggaggtaa gatcggtgtt gccgccattt 5760gtaatggtgg
tggtggtgct tcctctattg tcattgaaaa gatatgatta cgttctgcga
5820ttttctcatg atctttttca taaaatacat aaatatataa atggctttat
gtataacagg 5880cataatttaa agttttattt gcgattcatc gtttttcagg
tactcaaacg ctgaggtgtg 5940ccttttgact tacttttccg ccttggcaag
ctggccgggt gatacttgca caagttccac 6000taattactga catttgtggt
attaactcgt ttgactgctc tacaattgta ggatgttaat 6060caatgtcttg
gctgcctaac ctgcaggccg cgagcgccga tatgctatgt aatagacaat
6120aaaaccatgt ttatataaaa aaaattcaaa atagaaaacg attctgtaca
aggagtattt 6180tttttttgtt ctagtgtgtt tatattatcc ttggctaaga
ggcactgcgt atacttcaag 6240gtacccctgt gttttgaaaa aaaacaacag
taaaatagga actccgcgag gttcaggaac 6300ctgaaacaaa atcaataaaa
acattatatg cgtttcgaac aaaattaaag aaaaagaata 6360aatatagatt
aaaaaaaaaa agaagaaatt aaaagaattt ctactaaatc ccaattgtta
6420tatatttgtt aaatgccaaa aaagtttata aaaaatttag aatgtataaa
taataataaa 6480ctaagtaacg cgatcgccga cgccgccgat atctccctcg
ccagcggccg ccttatggct 6540aagaatgttg gaattttggc catggacatc
tacttcccac caacttgtgt tcagcaggag 6600gctttagaag cacatgacgg
agcctcaaag ggtaagtaca caatcggatt aggacaggat 6660tgcttagcat
tctgcactga attggaggac gtcatctcaa tgtctttcaa cgccgtcacc
6720tcattgttag agaagtacaa aatcgaccca aaccagatcg gaaggttgga
agtcggttct 6780gaaaccgtca tcgacaagtc taaatcaatc aagactttcg
ttatgcagtt gttcgaaaag 6840tgcggtaata ctgacgtcga gggtgtagac
tctactaacg cttgttatgg tggtaccgca 6900gctttattga actgcgtaaa
ctgggttgag tcaaactcat gggatggtag gtacggatta 6960gtcatttgca
ccgattctgc cgtctacgcc gagggtccag caaggccaac cggtggagct
7020gcagctattg ctatgttaat cggaccagat gcccctatag tcttcgagtc
taagttgagg 7080ggttcacaca tccctaacgt ctacgacttc tacaagccaa
acttggcctc agagtatcca 7140gttgtcgacg gaaagttatc tcagacatgc
tacttgatgg ccttagattc atgttacaag 7200cacttatgca acaagttcga
aaagttggag ggaaaggagt tctcaattaa cgacgccgac 7260tacttcgttt
ttcactctcc atacaacaaa ttggtccaga agtcattcgc caggttattg
7320tacaacgatt ttttgagaaa cgcatcatct atcgatgagg ccgccaagga
gaaattcacc 7380ccatattctt ctttgtcatt ggacgagtct taccagtcta
gggacttgga gaaggtatca 7440cagcaattgg ctaaaacctt ctatgacgcc
aaagttcagc caaccacctt ggtccctaaa 7500caggtcggaa atatgtatac
tgcatctttg tatgccgcct ttgcctcttt gatccacaac 7560aagcacaacg
atttagtcgg aaaaagggtt gtcatgtttt cttacggtgc cggatctact
7620gccactatgt tctcattgag gttatgcgaa aaccagtcac cattttcatt
gtctaacatc 7680gcctcagtca tggacgtagg tgtctcacct gagaagttcg
tagaaaccat gaagttgatg 7740gagcacagat acggtgccaa agaattcgtc
acttcaaaag agggaatctt ggatttgttg 7800gccccaggaa cctactattt
gaaggaggtc gactctttgt acagaaggtt ctatggaaag 7860aagggagacg
acggatctgt cgcaaacggt cagtaaatcg gcggcgtcgg cgatcgcgtt
7920aaggcggccg ctggcgaggg agatatttca acctgggcct aacagtaaag
atatcctcct 7980caaaactggt gcacttaatc gctgaatttg ttctggcttc
tcttcttttt ctttattccc 8040cccatgggcc aaaaaaaata gtactatcag
gaatttggcg ccgggtcacg atatacgtgt 8100acagtgacct aggcgacgcc
acaaggaaaa aggaaaaaaa cagaaaaaac aacaaaaact 8160aaaacaaaca
cgaaaacttt aatagatcta agtgaagtag tggtgaggca attggagtga
8220catagcagct actacaacta caaaaaaggc gcgccacggt cgtgcggata
tgaaagaggt 8280cgttatagct tctgccgtca ggaccgccat cggatcttac
ggtaagtcat taaaggacgt 8340ccctgccgtt gatttaggag ccaccgcaat
taaagaggcc gttaaaaagg caggtataaa 8400gccagaggac gtcaacgagg
tcatcttggg aaatgtctta caagccggat taggtcaaaa 8460cccagcaaga
caagcatcat tcaaagccgg tttacctgtc gagatacctg caatgaccat
8520caacaaggtt tgcggttcag gattaaggac cgtttcttta gcagcacaga
tcattaaggc 8580tggagatgca gacgttatca ttgctggtgg tatggaaaac
atgtcaagag ccccatactt 8640ggctaataac gccaggtggg gatataggat
gggaaacgcc aagtttgtcg acgaaatgat 8700tactgacgga ttgtgggacg
ccttcaatga ctatcacatg ggtataaccg cagaaaacat 8760tgccgagagg
tggaatatct caagagaaga acaggatgag tttgcattgg cctcacagaa
8820aaaagcagag gaggcaataa agtcaggtca gtttaaggat gaaatcgtcc
cagtcgtcat 8880caagggaaga aagggtgaga cagttgtcga caccgacgaa
caccctagat ttggttcaac 8940catcgaggga ttagcaaagt tgaagccagc
cttcaagaaa gacggaaccg taaccgccgg 9000taatgcatct ggattgaacg
attgcgcagc agttttggtc ataatgtcag ccgagaaagc 9060taaggagttg
ggtgtcaagc cattggcaaa aattgtttca tacggatcag ccggtgtcga
9120ccctgccatc atgggttacg gaccttttta cgccaccaag gctgcaatcg
aaaaggccgg 9180ttggaccgta gatgaattgg atttgatcga gtcaaacgag
gcctttgccg cccaatcatt 9240ggctgtcgcc aaggacttga agttcgacat
gaacaaggtc aacgtcaacg gtggtgccat 9300cgcattgggt caccctatcg
gagcctctgg tgccaggatc ttggttacct tggtccacgc 9360catgcagaag
agggacgcaa agaagggttt ggccaccttg tgcatcggtg gaggtcaggg
9420aacagctatc ttgttagaga aatgcagccc ctcagccccc ctagcgtcga
ataaaagaca 9480ttggtacatg atatcaaaca gaattttaac atttcttgat
ccagtttgta aacaaaacaa 9540acaatttttc taccatttaa cttcatacca
tcggcgagag ccgaacagga aaaaaaagaa 9600gtctccggtt atcgtaagca
gtatcaaata ataagaatgt atgtgtgtgc aatttgttat 9660acccacgaag
aagtgcgcag tagagttaga aaaccaactg agtaatcttt actcccgaca
9720atcgtccaat aatcctcttg ttgctaggaa cgtgatgatg gatttcgttt
gaaatccgga 9780cggaaaactc aaaagaagtc caaccaccaa ccattttcga
gcctcaagaa tctctaagca 9840ggtttcttta ctaaggggat ggcctttctg
tcctggacat tttttccttc cttttttcat 9900ttccttgaaa ggaacagatt
ttttttgact tttgccacac agctgcacta tctcaacccc 9960ttttacattt
taagttttcg ggttgaatgg ccggtgttta aaccccagcg cctggcggg
10019275027DNAArtificial SequenceSynthetic Mat alpha FcphI target
site cassette 27tgatgtctgg gttttgtttg ggatgcaatt tattgcttcc
caatgtagaa aagtacatca 60tatgaaacaa cttaaactct taactacttc ttttaacctt
cactttttat gaaatgtatc 120aaccatatat aataacttaa tagacgacat
tcacaatatg tttacttcga agcctgcttt 180caaaattaag aacaaagcat
ccaaatcata cagaaacaca gcggtttcaa aaaagctgaa 240agaaaaacgt
ctagctgagc atgtgaggcc aagctgcttc aatattattc gaccactcaa
300gaaagatatc cagattcctg ttccttcctc tcgattttta aataaaatcc
aaattcacag 360gatagcgtct ggaagtcaaa atactcagtt tcgacagttc
aataagacat ctataaaatc 420ttcaaagaaa tatttaaact catttatggc
ttttagagca tattactcac agtttggctc 480cggtgtaaaa caaaatgtct
tgtcttctct gctcgctgaa gaatggcacg cggacaaaat 540gcagcacgga
atatgggact acttcgcgca acagtataat tttataaacc ctggttttgg
600ttttgtagag tggttgacga ataattatgc tgaagtacgt ggtgacggat
attgggaaga 660tgtgtttgta catttggcct tatagagtgt ggtcgtggcg
gaggttgttt atctttcgag 720tactgaatgt tgtcagtata gctatcctat
ttgaaactcc ccatcgtctt gctcttgttc 780ccaatgtttg tttatacact
catatggcta tacccttatc tacttgcctc ttttgtttat 840gtctatgtat
ttgtataaaa tatgatatta ctcagactca agcaaacaat caatttgtga
900gcgttgcgct cgtgcatcga gaagttaaga ttatatgaat aactaaatac
taaatagaaa 960tgtaaataca gtgagaacaa aacaaaaaaa aacgaacaga
gaaactaaat ccacattaat 1020tgagagttct atctattaga aaatgcaaac
tccaactaaa tgggaaaaca gataacctct 1080tttatttttt tttaatgttt
gatattcgag tctttttctt ttgttaggtt tatattcatc 1140atttcaatga
ataaaagaag cttcttattt tggttgcaaa gaatgaaaaa aaaggatttt
1200ttcatacttc taaagcttca attataacca aaaattttat aaatgaagag
aaaaaatcta 1260gtagtatcaa gttaaactta gaaaaactca tcgagcatca
aatgaaactg caatttattc 1320atatcaggat tatcaatacc atatttttga
aaaagccgtt tctgtaatga aggagaaaac 1380tcaccgaggc agttccatag
gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 1440ccaacatcaa
tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
1500tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat
ttctttccag 1560acttgttcaa caggccagcc attacgctcg tcatcaaaat
cactcgcatc aaccaaaccg 1620ttattcattc gtgattgcgc ctgagcgaga
cgaaatacgc gatcgctgtt aaaaggacaa 1680ttacaaacag gaatcgaatg
caaccggcgc aggaacactg ccagcgcatc aacaatattt 1740tcacctgaat
caggatattc ttctaatacc tggaatgctg ttttgccggg gatcgcagtg
1800gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
aagaggcata 1860aattccgtca gccagtttag tctgaccatc tcatctgtaa
catcattggc aacgctacct 1920ttgccatgtt tcagaaacaa ctctggcgca
tcgggcttcc catacaatcg atagattgtc 1980gcacctgatt gcccgacatt
atcgcgagcc catttatacc catataaatc agcatccatg 2040ttggaattta
atcgcggcct cgaaacgtga gtcttttcct tacccatttt taatgttact
2100tctcttgcag ttagggaact ataatgtaac tcaaaataag attaaacaaa
ctaaaataaa 2160aagaagttat acagaaaaac ccatataaac cagtactaat
ccataataat aatacacaaa 2220aaaactatca aataaaacca gaaaacagat
tgaatagaaa aattttttcg atctcctttt 2280atattcaaaa ttcgatatat
gaaaaaggga actctcagaa aatcaccaaa tcaatttaat 2340tagatttttc
ttttccttct agcgttggaa agaaaaattt ttcttttttt ttttagaaat
2400gaaaaatttt tgccgtagga atcaccgtat aaaccctgta taaacgctac
tctgttcacc 2460tgtgtaggct atgattgacc cagtgttcat tgttattgcg
agagagcggg agaaaagaac 2520cgatacaaga gatccatgct ggtatagttg
tctgtccaac actttgatga acttgtagga 2580cgatgatgtg tattactagt
gtcgacatgc gtccatcttt acagtcctgt cttattgttc 2640ttgatttgtg
ccccgtaaaa tactgttact tggttctggc gaggtattgg atagttcctt
2700tttataaagg ccatgaagct ttttctttcc aatttttttt ttttcgtcat
tatagaaatc 2760attacgaccg agattcccgg gtaataactg atataattaa
attgaagctc taatttgtga 2820gtttagtata catgcattta cttataatac
agttttttag ttttgctggc cgcatcttct 2880caaatatgct tcccagcctg
cttttctgta acgttcaccc tctaccttag catcccttcc 2940ctttgcaaat
agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
3000cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
caccgggtgt 3060cataatcaac caatcgtaac cttcatctct tccacccatg
tctctttgag caataaagcc 3120gataacaaaa tctttgtcac tcttcgcaat
gtcaacagta cccttagtat attctccagt 3180agatagggag cccttgcatg
acaattctgc taacatcaaa aggcctctag gttcctttgt 3240tacttcttct
gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc
3300attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
gcaatttgac 3360tgtattacca atgtcagcaa attttctgtc ttcgaagagt
aaaaaattgt acttggcgga 3420taatgccttt agcggcttaa ctgtgccctc
catggaaaaa tcagtcaaga tatccacatg 3480tgtttttagt aaacaaattt
tgggacctaa tgcttcaact aactccagta attccttggt 3540ggtacgaaca
tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag
3600cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
ctttcgacat 3660gatttatctt cgtttcctgc aggtttttgt tctgtgcagt
tgggttaaga atactgggca 3720atttcatgtt tcttcaacac cacatatgcg
tatatatacc aatctaagtc tgtgctcctt 3780ccttcgttct tccttctgct
cggagattac cgaatcaaaa aaatttcaaa gaaaccggaa 3840tcaaaaaaaa
gaacaaaaaa aaaaaagatg aattgaaaag ctttatggac cctgaaacca
3900cagccacatt aaccttcttt gatggtcaaa acttatcctt caccataaat
atgcctcgca 3960aaaaaggtaa ttaacatata tagaattaca ttatttatga
aatatcatca ctatctctta 4020gcatctttaa tccttttcta catcagataa
cttcggtttg ttatcatcgt ctgtattgtc 4080atcaattggc gcagtagcct
caatttcaac gtcgtttgac tctggtgttt gttcatgtgc 4140agatccatga
gatgatgaac ttgtgagcgt tgcgctcgtg catccggagg ttgtttatct
4200ttcgagtact gaatgttgtc agtatagcta tcctatttga aactccccat
cgtcttgctc 4260ttgttcccaa tgtttgttta tacactcata tggctatacc
cttatctact tgcctctttt 4320gtttatgtct atgtatttgt ataaaatatg
atattactca gactcaagca aacaatcaat 4380tcttagcatc attctttgtt
cttatcttaa ccataaacga tcttgatgtg acttttgtaa 4440tttgaacgaa
ttggctatac gggacggatg acaaatgcac cattactcta ggttgttgtt
4500ggatcttaac aaaccgtaaa ggtaaactgc ccatgcggtt cacatgactt
ttgactttcc 4560tttgtttgct agttaccttc ggcttcacaa tttgtttttc
cacttttcta acaggtttat 4620cacctttcaa acttatcttt atcttattcg
ccttcttggg tgcctccaca gtagaggtta 4680cttccttttt aatatgtact
tttaggatac tttcacgctt tataacaata tcaagtttac 4740cttcttcatt
actattcatc ttcgccacaa gtcttctctc ccttggtgtt tccaatctaa
4800ctacaaaact gttgattagg gtgtacatca ccctaacaag atcatgtatt
tgcttcctct 4860ggtacaagct aagaacaggt aaattcaaaa catcccagag
taatatcttc aaagggctat 4920accctttaaa catatctcgg catatttgta
ttaacccact aatattttga cggccaatct 4980tttctatttt tattttcata
tcatcgacgt aatgaccact taaaaac 5027286126DNAArtificial
SequenceSynthetic ADS_GAL80 donor cassette 28gcctgtctac aggataaaga
cgggtcggat acctgcacaa gcaatttggc acctgcatac 60cccatttccc cagtagataa
cttcaacaca cacatcaatg tccctcacca gtttatttcc 120aaaagagacg
ctttttacta cctgactaga ttttcatttt gtttcttttg gattgcgctt
180gcctttgtag gtgtgtcgtt tatcctttac gttttgactt ggtgctcgaa
gatgctttca 240gagatggtgc ttatcctcat gtcttttggg tttgtcttca
atacggcagc cgttgtcttg 300caaacggccg cctctgccat ggcaaagaat
gctttccatg acgatcatcg tagtgcccaa 360ttgggtgcct ctatgatggg
tttaaacgta tggcttgggc aagtgtcttt ttatgtatcg 420tggaatttat
cctgctggtc ttctggtctg ttagggcaag gttggcctct acttactcca
480tcgacaattc aagatacaga acctcctcca gatggaatcc cttccataga
gagaaggagc 540aagcaactga cccaatattg actgccactg gacctgaaga
catgcaacaa agtgcaagca 600tagtggggcc ttcttccaat gctaatccgg
tcactgccac tgctgctacg gaaaaccaac 660ctaaaggtat taacttcttc
actataagaa aatcacacga gcgcccggac gatgtctctg 720tttaaatggc
gcaagttttc cgctttgtaa tatatattta tacccctttc ttctctcccc
780tgcaatataa tagtttaatt ctaatattaa taatatccta tattttcttc
atttaccggc 840gcactctcgc ccgaacgacc tcaaaatgtc tgctacattc
ataataacca aaagctcata 900actttttttt ttgaacctga atatatatac
atcacatatc actgctggtc ctcttcgagc 960gtcccaaaac cttctcaagc
aaggttttca gtataatgtt acatgcgtac acgcgtctgt 1020acagaaaaaa
aagaaaaatt tgaaatataa ataacgttct taatactaac ataactataa
1080aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
gttagagcgg 1140atcttagcta gcttaaatag acatgggata aactaataaa
gacttaatta aatgcttgta 1200ctcatctccc atacgagtga agttatcttt
acccgcgtac tgcacttcca ggaattgaca 1260aagatagata actgccatca
gtaatggtct gggtatgttt ttagtggtca agtattctct 1320gtttatgtcc
ttccaaacgt cttcgacttc cttgtatatg agggtttgtg cgtattcctc
1380atttacatta tattccttca tataagattc tagagaagat gatgagtgct
ttctctcctg 1440ttctgccttg tgcgtcatca agtcgtttag acgacgaccc
aaaataccag aataccggaa 1500tagtggtggc gctgacacgg cccattcaac
tgattcttta gtgaaaatat ctgacatccc 1560caggtaacat gtggttgtca
gtaggttcgc tccaccagtt ataataacaa ctggatcgtg 1620ttcttcagtg
gtcggtatgt gcccttcgtt tgcccacttt gcttcgacca tgagattccg
1680tacaaattcc ttaacaaact cttttccgca attaaataaa tctgttctcc
cttcttttgc 1740aagaaattcc tccatttctg tatacgtatc catgaataat
ttatagatag gcttcatgta 1800ttctggcagc gtatcaaggc aggtaatcga
ccaacgttct acagcttcag tgaaaatttt 1860taattcttca tatgttccat
aagcatcgta agtatcatca atcagggtta taacggctac 1920cactttggtg
aagaaaactc gtgctcttga atattgtggt tcataaccag aacctaaacc
1980ccagaaataa cactctacaa tgcggtcgcg caaacaaggt gcgttctttt
ttatatcaaa 2040tgctttccac catttacaga cgtggctaag ctcctccttg
tgcaaagatt gcagcaggtt 2100gaattctaat tttgctaatt ttaaaagcgt
cttattatga gagtcttgtt gttggtagaa 2160aggaatatac tgagcagctt
ctatgcgggg aagacgcttc cataaaggtt gttttaatgc 2220tctttgtatt
tcggtaaaca aagccggatt agtggagaat gcatccttag tcattataga
2280taatcgactt ctagtaaacc caagcgcgtc ttccaaaata atttcgcctg
gaacccgcat 2340ggaagtcgcc tcatataact ctaataaccc ttccacatca
ttagccaatg attgtttaaa 2400agcaccgttt ttatctttgt aattattgaa
aacatcacag gtcacgtagt agccttgctt 2460cctcatcaag cgaaaccata
gtgaactgcg atctccattc cagttgtctc cgtatgtctc 2520gtaaatacat
tgaagagcat ggtcaatttc cctttcaaaa tggtatggaa ttcctagtct
2580ctgtatttca tctattaatt ttaacaaatt agcatgcttc ataggaatgt
ctaaggcttc 2640cttcaggagc tgtcttactt ctttcttcag atcgtttaca
atttgttcga ccccttgttc 2700tacttgtttc tcataaatga gaaactgatc
accccaaata gagggaggga aattggctat 2760aggtctaatc ggtttctcct
cggttagtgc catggatcct tcagttacag taaatgtttt 2820agttgcatcg
tcataggtcc attcaccatc aacaccatta tcattagcat attgcttaaa
2880gaccttttcg gctgttgctg catctactgc ttcggtagta gtttcaccct
tcaaagtttt 2940accattcaaa attaatttat attgacccat ttatattgaa
ttttcaaaaa ttcttacttt 3000ttttttggat ggacgcaaag aagtttaata
atcatattac atggcattac caccatatac 3060atatccatat ctaatcttac
ttatatgttg tggaaatgta aagagcccca ttatcttagc 3120ctaaaaaaac
cttctctttg gaactttcag taatacgctt aactgctcat tgctatattg
3180aagtacggat tagaagccgc cgagcgggcg acagccctcc gacggaagac
tctcctccgt 3240gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat
gtgcctcgcg ccgcactgct 3300ccgaacaata aagattctac aatactagct
tttatggtta tgaagaggaa aaattggcag 3360taacctggcc ccacaaacct
tcaaattaac gaatcaaatt aacaaccata ggatgataat 3420gcgattagtt
ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat
3480ctattaacag atatataaat ggaaaagctg cataaccact ttaactaata
ctttcaacat 3540tttcagtttg tattacttct tattcaaatg tcataaaagt
atcaacaaaa aattgttaat 3600atacctctat actttaacgt caaggagaaa
aaactataat gtcattaccg ttcttaactt 3660ctgcaccggg aaaggttatt
atttttggtg aacactctgc tgtgtacaac aagcctgccg 3720tcgctgctag
tgtgtctgcg ttgagaacct acctgctaat aagcgagtca tctgcaccag
3780atactattga attggacttc ccggacatta gctttaatca taagtggtcc
atcaatgatt 3840tcaatgccat caccgaggat caagtaaact cccaaaaatt
ggccaaggct caacaagcca 3900ccgatggctt gtctcaggaa ctcgttagtc
ttttggatcc gttgttagct caactatccg 3960aatccttcca ctaccatgca
gcgttttgtt tcctgtatat gtttgtttgc ctatgccccc 4020atgccaagaa
tattaagttt tctttaaagt ctactttacc catcggtgct gggttgggct
4080caagcgcctc tatttctgta tcactggcct tagctatggc ctacttgggg
gggttaatag 4140gatctaatga cttggaaaag ctgtcagaaa acgataagca
tatagtgaat caatgggcct 4200tcataggtga aaagtgtatt cacggtaccc
cttcaggaat agataacgct gtggccactt 4260atggtaatgc cctgctattt
gaaaaagact cacataatgg aacaataaac acaaacaatt 4320ttaagttctt
agatgatttc ccagccattc caatgatcct aacctatact agaattccaa
4380ggtctacaaa agatcttgtt gctcgcgttc gtgtgttggt caccgagaaa
tttcctgaag 4440ttatgaagcc aattctagat gccatgggtg aatgtgccct
acaaggctta gagatcatga 4500ctaagttaag taaatgtaaa ggcaccgatg
acgaggctgt agaaactaat aatgaactgt 4560atgaacaact attggaattg
ataagaataa atcatggact gcttgtctca atcggtgttt 4620ctcatcctgg
attagaactt attaaaaatc tgagcgatga tttgagaatt ggctccacaa
4680aacttaccgg tgctggtggc ggcggttgct ctttgacttt gttacgaaga
gacattactc 4740aagagcaaat tgacagtttc aaaaagaaat tgcaagatga
ttttagttac gagacatttg 4800aaacagactt gggtgggact ggctgctgtt
tgttaagcgc aaaaaatttg aataaagatc 4860ttaaaatcaa atccctagta
ttccaattat ttgaaaataa aactaccaca aagcaacaaa 4920ttgacgatct
attattgcca ggaaacacga atttaccatg gacttcataa gctaatttgc
4980gataggcatt atttattagt tgtttttaat cttaactgtg tatgaagttt
tatgtaataa 5040agatagaaag agaaacaaaa aaaaattttt cgtagtatca
attcagcttt cgaagacaga 5100atgaaattta agcagaccat tcatcttgcc
ctgtgcttgg cccccagtgc agcgaacgtt 5160ataaaaacga atactgagta
tatatctatg taaaacaacc atatcatttc ttgttctgaa 5220ctttgtttac
ctaactagtt ttaaatttcc ctttttcgtg catgcgggtg ttcttattta
5280ttagcatact acatttgaaa tatcaaattt ccttagtaga aaagtgagag
aaggtgcact 5340gacacaaaaa ataaaatgct acgtataact gtcaaaactt
tgcagcagcg ggcatccttc 5400catcatagct tcaaacatat tagcgttcct
gatcttcata cccgtgctca aaatgatcaa 5460acaaactgtt attgccaaga
aataaacgca aggctgcctt caaaaactga tccattagat 5520cctcatatca
agcttcctca tagaacgccc aattacaata agcatgtttt gctgttatca
5580ccgggtgata ggtttgctca accatggaag gtagcatgga atcataattt
ggatactaat 5640acaaatcggc catataatgc cattagtaaa ttgcgctccc
atttaggtgg ttctccaggc 5700aaatttgaat actaataaat gcggtgcatt
tgcaaaatga atttattcca aggccaaaac 5760aacacgatga atggctttat
ttttttgtta ttcctgacat gaagctttat gtaattaagg 5820aaacggacat
cgaggaattt gcatcttttt tagatgaagg agctattcaa gcaccaaagc
5880tatccttcca ggattattta agcggtaagg ccaaggcttc ccaacaggtt
catgaagtgc 5940atcatagaaa gcttacaagg tttcagggtg aaacttttct
aagagattgg aacttagtct 6000gtgggcatta taagagagat gctaagtgtg
gagaaatggg acccgacata attgcagcat 6060ttcaagatga aaagcttttt
cctgagaata atctagcctt aatttctcat attgggggtc 6120atattt
6126299550DNAArtificial SequenceSynthetic ADS_HXT3 donor cassette
29accggtatat caaatggcgg tgtagtttga aaagtacact gtatgtccat taataaaatt
60acgccgaaga acactgacta gctataaatt ctcttccggc gtccaatttc aacctaaggc
120aagtattgtt aatcaataat gcgtgttggt aaatatgaag cccgatgttg
attaagaaga 180attattcgta taagaattaa gcaagcaaaa ttaaggaaaa
ttttttcttt cctattcgtc 240atcgcagaca gccttcatct tctcgagata
acacctggag gaggagcaat gaaatgaaag 300gaaaaaaaaa tactttcttt
ttcttgaaaa aagaaaaaaa ttgtaagatg agctattcgc 360ggaacattct
agctcgtttg catcttcttg catttggtag gttttcaata gttcggtaat
420attaacggat acctactatt atcccctagt aggctctttt cacggagaaa
ttcgggagtg 480ttttttttcc gtgcgcattt tcttagctat attcttccag
cttcgcctgc tgcccggtca 540tcgttcctgt cacgtagttt ttccggattc
gtccggctca tataataccg caataaacac 600ggaatatctc gttccgcgga
ttcggttaaa ctctcggtcg cggattatca cagagaaagc 660ttcgtggaga
atttttccag attttccgct ttccccgatg ttggtatttc cggaggtcat
720tatactgacc gccattataa tgactgtaca acgaccttct ggagaaagaa
acaactcaat 780aacgatgtgg gacattgggg gcccactcaa aaaatctggg
gactatatcc ccagagaatt 840tctccagaag agaagaaaag tcaaagtttt
ttttcgcttg ggggttgcat ataaacttcg 900agcgtcccaa aaccttctca
agcaaggttt tcagtataat gttacatgcg tacacgcgtc 960tgtacagaaa
aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
1020taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt
tcggttagag 1080cggatcttag atagacatag ggtagaccaa caaagactta
attaagtgct tatattcgtc 1140acccattctg gtgaaattgt ctttaccagc
gtattgaact tctaagaatt gacataaata 1200gataacagcc attaacaatg
gacgtggaat atttttggtg gtcaagtatt ctctgttaat 1260atctttccaa
acgtcttcaa cttccttgta aatcaaagtt tgagcgtatt cttcgttaac
1320gttgtattcc ttcatgtaag attctaagga agaggaggag tgctttcttt
cttgctcagc 1380cttgtgagtc atcaaatcgt ttaatcttct acccaaaata
ccagagtatc tgaacaaagg 1440tggagcggaa acggcccatt caacagattc
cttggtgaaa atatcagaca tacctaagta 1500acaagtagta gtcaacaagt
tagcaccacc agtaatgata acgactggat cgtgttcttc 1560agtagttgga
atatgacctt cgttagccca tttagcttca accatcaagt tacgaacgaa
1620ttccttaacg aattccttac cacaattgaa caagtcggta cgaccttcct
tagccaagaa 1680ttcttccatt tcggtgtaag tgtccatgaa caacttataa
attggcttca tgtattctgg 1740taaagtatct aaacaggtaa tggaccatct
ttcgacagct tcagtgaaaa tcttcaattc 1800ttcgtaggta ccgtaagcgt
cgtaagtgtc gtcaatcaag gtaataacag caaccacctt 1860agtaaagaaa
actctagctc tagagtattg tggttcgtaa ccggaaccca aaccccagaa
1920gtaacattcg acaattctat ctctcaaaca tggagcgttc ttcttaatat
cgaaggcttt 1980ccaccactta caaacgtgag acaattcttc cttgtgcaag
gattgcaaca agttaaattc 2040caatttagcc aatttcaaca aggttttgtt
gtgagagtct tgttgttggt agaatgggat 2100gtattgagca gcttcgattc
ttggcaaacg cttccacaat ggttgtttca aagctctttg 2160gatttcagtg
aacaaagctg ggttagtaga gaaggcatct ttagtcatga tggacaatct
2220agatctagta aaacccaaag cgtcttccaa gatgatttca ccaggaactc
tcatggaggt 2280ggcttcatac aattctaaca aaccttcaac atcgttggcc
aaagattgct taaaagcacc 2340attcttatct ttgtagttgt tgaaaacgtc
acaagtaacg tagtaacctt gctttctcat 2400taatctgaac cataaggaag
atctgtcacc gttccagtta tcaccgtaag tttcgtaaat 2460acattgcaaa
gcgtgatcaa tttctctttc gaaatggtat ggaataccta atctttggat
2520ttcgtcgatt aatttcaata agttagcgtg cttcattggg atgtccaaag
cttccttcaa 2580caattgtcta acttccttct tcaaatcgtt aacgatttgt
tcaacacctt gctcaacttg 2640cttttcgtag atcaagaatt ggtcacccca
gatagaaggt ggaaaattag caattggtct 2700gattggtttt tcttcagtca
aagccatgga tccttcagtt acagtaaatg ttttagttgc 2760atcgtcatag
gtccattcac catcaacacc attatcatta gcatattgct taaagacctt
2820ttcggctgtt gctgcatcta ctgcttcggt agtagtttca cccttcaaag
ttttaccatt 2880caaaattaat ttatattgac ccatttatat tgaattttca
aaaattctta cttttttttt 2940ggatggacgc aaagaagttt aataatcata
ttacatggca ttaccaccat atacatatcc 3000atatctaatc ttacttatat
gttgtggaaa tgtaaagagc cccattatct tagcctaaaa 3060aaaccttctc
tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
3120ggattagaag ccgccgagcg ggcgacagcc ctccgacgga agactctcct
ccgtgcgtcc 3180tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct
cgcgccgcac tgctccgaac 3240aataaagatt ctacaatact agcttttatg
gttatgaaga ggaaaaattg gcagtaacct 3300ggccccacaa accttcaaat
taacgaatca aattaacaac cataggatga taatgcgatt 3360agttttttag
ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
3420acagatatat aaatggaaaa gctgcataac cactttaact aatactttca
acattttcag 3480tttgtattac ttcttattca aatgtcataa aagtatcaac
aaaaaattgt taatatttta 3540attctgctgt aacccgtaca tgcccaaaat
agggggcggg ttacacagaa tatataacat 3600cgtaggtgtc tgggtgaaca
gtttattcct ggcatccact aaatataatg gagcccgctt 3660tttaagctgg
catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca
3720accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg
cacaaacagg 3780caaaaaacgg gcacaacctc aatggagtga tgcaacctgc
ctggagtaaa tgatgacaca 3840aggcaattga cccacgcatg tatctatctc
attttcttac accttctatt accttctgct 3900ctctctgatt tggaaaaagc
tgaaaaaaaa ggttgaaacc agttccctga aattattccc 3960ctacttgact
aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
4020tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt
aaaacaccaa 4080gaacttagtt tcgatccccg cgtgcttggc cggccgtatc
cccgcgtgct tggccggccg 4140tatgtctcag aacgtttaca ttgtatcgac
tgccagaacc ccaattggtt cattccaggg 4200ttctctatcc tccaagacag
cagtggaatt gggtgctgtt gctttaaaag gcgccttggc 4260taaggttcca
gaattggatg catccaagga ttttgacgaa attatttttg gtaacgttct
4320ttctgccaat ttgggccaag ctccggccag acaagttgct ttggctgccg
gtttgagtaa 4380tcatatcgtt gcaagcacag ttaacaaggt ctgtgcatcc
gctatgaagg caatcatttt 4440gggtgctcaa tccatcaaat gtggtaatgc
tgatgttgtc gtagctggtg gttgtgaatc 4500tatgactaac gcaccatact
acatgccagc agcccgtgcg ggtgccaaat ttggccaaac 4560tgttcttgtt
gatggtgtcg aaagagatgg gttgaacgat gcgtacgatg gtctagccat
4620gggtgtacac gcagaaaagt gtgcccgtga ttgggatatt actagagaac
aacaagacaa 4680ttttgccatc gaatcctacc aaaaatctca aaaatctcaa
aaggaaggta aattcgacaa 4740tgaaattgta cctgttacca ttaagggatt
tagaggtaag cctgatactc aagtcacgaa 4800ggacgaggaa cctgctagat
tacacgttga aaaattgaga tctgcaagga ctgttttcca 4860aaaagaaaac
ggtactgtta ctgccgctaa cgcttctcca atcaacgatg gtgctgcagc
4920cgtcatcttg gtttccgaaa aagttttgaa ggaaaagaat ttgaagcctt
tggctattat 4980caaaggttgg ggtgaggccg ctcatcaacc agctgatttt
acatgggctc catctcttgc 5040agttccaaag gctttgaaac atgctggcat
cgaagacatc aattctgttg attactttga 5100attcaatgaa gccttttcgg
ttgtcggttt ggtgaacact aagattttga agctagaccc 5160atctaaggtt
aatgtatatg gtggtgctgt tgctctaggt cacccattgg gttgttctgg
5220tgctagagtg gttgttacac tgctatccat cttacagcaa gaaggaggta
agatcggtgt 5280tgccgccatt tgtaatggtg gtggtggtgc ttcctctatt
gtcattgaaa agatatgatt 5340acgttctgcg attttctcat gatctttttc
ataaaataca taaatatata aatggcttta 5400tgtataacag gcataattta
aagttttatt tgcgattcat cgtttttcag gtactcaaac 5460gctgaggtgt
gccttttgac ttacttttcc gccttggcaa gctggccggg tgatacttgc
5520acaagttcca ctaattactg acatttgtgg tattaactcg tttgactgct
ctacaattgt 5580aggatgttaa tcaatgtctt ggctgcctaa cctgcaggcc
gcgagcgccg atatgctatg 5640taatagacaa taaaaccatg tttatataaa
aaaaattcaa aatagaaaac gattctgtac 5700aaggagtatt ttttttttgt
tctagtgtgt ttatattatc cttggctaag aggcactgcg 5760tatacttcaa
ggtacccctg tgttttgaaa aaaaacaaca gtaaaatagg aactccgcga
5820ggttcaggaa cctgaaacaa aatcaataaa aacattatat gcgtttcgaa
caaaattaaa 5880gaaaaagaat aaatatagat taaaaaaaaa aagaagaaat
taaaagaatt tctactaaat 5940cccaattgtt atatatttgt taaatgccaa
aaaagtttat aaaaaattta gaatgtataa 6000ataataataa actaagtaac
gcgatcgccg acgccgccga tatctccctc gccagcggcc 6060gccttatggc
taagaatgtt ggaattttgg ccatggacat ctacttccca ccaacttgtg
6120ttcagcagga ggctttagaa gcacatgacg gagcctcaaa gggtaagtac
acaatcggat 6180taggacagga ttgcttagca ttctgcactg aattggagga
cgtcatctca atgtctttca 6240acgccgtcac ctcattgtta gagaagtaca
aaatcgaccc aaaccagatc ggaaggttgg 6300aagtcggttc tgaaaccgtc
atcgacaagt ctaaatcaat caagactttc gttatgcagt 6360tgttcgaaaa
gtgcggtaat actgacgtcg agggtgtaga ctctactaac gcttgttatg
6420gtggtaccgc agctttattg aactgcgtaa actgggttga gtcaaactca
tgggatggta 6480ggtacggatt agtcatttgc accgattctg ccgtctacgc
cgagggtcca gcaaggccaa 6540ccggtggagc tgcagctatt gctatgttaa
tcggaccaga tgcccctata gtcttcgagt 6600ctaagttgag gggttcacac
atccctaacg tctacgactt ctacaagcca aacttggcct 6660cagagtatcc
agttgtcgac ggaaagttat ctcagacatg ctacttgatg gccttagatt
6720catgttacaa gcacttatgc aacaagttcg aaaagttgga gggaaaggag
ttctcaatta 6780acgacgccga ctacttcgtt tttcactctc catacaacaa
attggtccag aagtcattcg 6840ccaggttatt gtacaacgat tttttgagaa
acgcatcatc tatcgatgag gccgccaagg 6900agaaattcac cccatattct
tctttgtcat tggacgagtc ttaccagtct agggacttgg 6960agaaggtatc
acagcaattg gctaaaacct tctatgacgc caaagttcag ccaaccacct
7020tggtccctaa acaggtcgga aatatgtata ctgcatcttt gtatgccgcc
tttgcctctt 7080tgatccacaa caagcacaac gatttagtcg gaaaaagggt
tgtcatgttt tcttacggtg 7140ccggatctac tgccactatg ttctcattga
ggttatgcga aaaccagtca ccattttcat 7200tgtctaacat cgcctcagtc
atggacgtag gtgtctcacc tgagaagttc gtagaaacca 7260tgaagttgat
ggagcacaga tacggtgcca aagaattcgt cacttcaaaa gagggaatct
7320tggatttgtt ggccccagga acctactatt tgaaggaggt cgactctttg
tacagaaggt 7380tctatggaaa gaagggagac gacggatctg tcgcaaacgg
tcagtaaatc ggcggcgtcg 7440gcgatcgcgt taaggcggcc gctggcgagg
gagatatttc aacctgggcc taacagtaaa 7500gatatcctcc tcaaaactgg
tgcacttaat cgctgaattt gttctggctt ctcttctttt 7560tctttattcc
ccccatgggc caaaaaaaat agtactatca ggaatttggc gccgggtcac
7620gatatacgtg tacagtgacc taggcgacgc cacaaggaaa aaggaaaaaa
acagaaaaaa 7680caacaaaaac taaaacaaac acgaaaactt taatagatct
aagtgaagta gtggtgaggc 7740aattggagtg acatagcagc tactacaact
acaaaaaagg cgcgccacgg tcgtgcggat 7800atgaaagagg tcgttatagc
ttctgccgtc aggaccgcca tcggatctta cggtaagtca 7860ttaaaggacg
tccctgccgt tgatttagga gccaccgcaa ttaaagaggc cgttaaaaag
7920gcaggtataa agccagagga cgtcaacgag gtcatcttgg gaaatgtctt
acaagccgga 7980ttaggtcaaa acccagcaag acaagcatca ttcaaagccg
gtttacctgt cgagatacct 8040gcaatgacca tcaacaaggt ttgcggttca
ggattaagga ccgtttcttt agcagcacag 8100atcattaagg ctggagatgc
agacgttatc attgctggtg gtatggaaaa catgtcaaga 8160gccccatact
tggctaataa cgccaggtgg ggatatagga tgggaaacgc caagtttgtc
8220gacgaaatga ttactgacgg attgtgggac gccttcaatg actatcacat
gggtataacc 8280gcagaaaaca ttgccgagag gtggaatatc tcaagagaag
aacaggatga gtttgcattg 8340gcctcacaga aaaaagcaga ggaggcaata
aagtcaggtc agtttaagga tgaaatcgtc 8400ccagtcgtca tcaagggaag
aaagggtgag acagttgtcg acaccgacga acaccctaga 8460tttggttcaa
ccatcgaggg attagcaaag ttgaagccag ccttcaagaa agacggaacc
8520gtaaccgccg gtaatgcatc tggattgaac gattgcgcag cagttttggt
cataatgtca 8580gccgagaaag ctaaggagtt gggtgtcaag ccattggcaa
aaattgtttc atacggatca 8640gccggtgtcg accctgccat catgggttac
ggaccttttt acgccaccaa ggctgcaatc 8700gaaaaggccg gttggaccgt
agatgaattg gatttgatcg agtcaaacga ggcctttgcc 8760gcccaatcat
tggctgtcgc caaggacttg aagttcgaca tgaacaaggt caacgtcaac
8820ggtggtgcca tcgcattggg tcaccctatc ggagcctctg gtgccaggat
cttggttacc 8880ttggtccacg ccatgcagaa gagggacgca aagaagggtt
tggccacctt gtgcatcggt 8940ggaggtcagg gaacagctat cttgttagag
aaatgcagcc cctcagcccc cctagcgtcg 9000aataaaagac attggtacat
gatatcaaac agaattttaa catttcttga tccagtttgt 9060aaacaaaaca
aacaattttt ctaccattta acttcatacc atcggcgaga gccgaacagg
9120aaaaaaaaga agtctccggt tatcgtaagc agtatcaaat aataagaatg
tatgtgtgtg 9180caatttgtta tacccacgaa gaagtgcgca gtagagttag
aaaaccaact gagtaatctt 9240tactcccgac aatcgtccaa taatcctctt
gttgctagga acgtgatgat ggatttcgtt 9300tgaaatccgg acggaaaact
caaaagaagt ccaaccacca accattttcg agcctcaaga 9360atctctaagc
aggtttcttt actaagggga tggcctttct gtcctggaca ttttttcctt
9420ccttttttca tttccttgaa aggaacagat tttttttgac ttttgccaca
cagctgcact 9480atctcaaccc cttttacatt ttaagttttc gggttgaatg
gccggtgttt aaaccccagc 9540gcctggcggg 9550304429DNAArtificial
SequenceSynthetic ADS_Mat alpha donor cassette 30tgatgtctgg
gttttgtttg ggatgcaatt tattgcttcc caatgtagaa aagtacatca 60tatgaaacaa
cttaaactct taactacttc ttttaacctt cactttttat gaaatgtatc
120aaccatatat aataacttaa tagacgacat tcacaatatg tttacttcga
agcctgcttt 180caaaattaag aacaaagcat ccaaatcata cagaaacaca
gcggtttcaa aaaagctgaa 240agaaaaacgt ctagctgagc atgtgaggcc
aagctgcttc aatattattc gaccactcaa 300gaaagatatc cagattcctg
ttccttcctc tcgattttta aataaaatcc aaattcacag 360gatagcgtct
ggaagtcaaa atactcagtt tcgacagttc aataagacat ctataaaatc
420ttcaaagaaa tatttaaact catttatggc ttttagagca tattactcac
agtttggctc 480cggtgtaaaa caaaatgtct tgtcttctct gctcgctgaa
gaatggcacg cggacaaaat 540gcagcacgga atatgggact acttcgcgca
acagtataat tttataaacc ctggttttgg 600ttttgtagag tggttgacga
ataattatgc tgaagtacgt ggtgacggat attgggaaga 660tgtgtttgta
catttggcct tatagagtgt ggtcgtggcg gaggttgttt atctttcgag
720tactgaatgt tgtcagtata gctatcctat ttgaaactcc ccatcgtctt
gctcttgttc 780ccaatgtttg tttatacact catatggcta tacccttatc
tacttgcctc ttttgtttat 840gtctatgtat ttgtataaaa tatgatatta
ctcagactca agcaaacaat caattttcaa 900aaattcttac tttttttttg
gatggacgca aagaagttta ataatcatat tacatggcat 960taccaccata
tacatatcca tatacatatc catatctaat cttacttata tgttgtggaa
1020atgtaaagag ccccattatc ttagcctaaa aaaaccttct ctttggaact
ttcagtaata 1080cgcttaactg ctcattgcta tattgaagta cggattagaa
gccgccgagc gggtgacagc 1140cctccgaagg aagactctcc tccgtgcgtc
ctcgtcttca ccggtcgcgt tcctgaaacg 1200cagatgtgcc tcgcgccgca
ctgctccgaa caataaagat tctacaatac tagcttttat 1260ggttatgaag
aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc
1320aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc
tggggtaatt 1380aatcagcgaa gcgatgattt ttgatctatt aacagatata
taaatgcaaa aactgcataa 1440ccactttaac taatactttc aacattttcg
gtttgtatta cttcttattc aaatgtaata 1500aaagtatcaa caaaaaattg
ttaatatacc tctatacttt aacgtcaagg agaaaaaacc 1560ccggatccat
gggtcaatat aaattaattt tgaatggtaa aactttgaag ggtgaaacta
1620ctaccgaagc agtagatgca gcaacagccg aaaaggtctt taagcaatat
gctaatgata 1680atggtgttga tggtgaatgg acctatgacg atgcaactaa
aacatttact gtaactgaag 1740gatccatggc cttaacagaa gagaagccaa
tcagacccat agccaacttc cctccatcaa 1800tatggggtga tcaatttctt
atatacgaaa agcaagttga acaaggtgtc gaacaaatcg 1860tcaacgatct
aaagaaggag gtcaggcagt tattaaaaga agctctggac ataccgatga
1920aacatgctaa tctactaaaa ttaattgatg agattcaaag attgggcatc
ccttatcact 1980tcgagagaga gattgatcat gcattgcaat gtatatacga
gacgtacggt gataactgga 2040acggagatag gagtagtcta tggttcaggt
tgatgagaaa gcaaggctac tatgtgacat 2100gcgatgtctt taataactat
aaggacaaaa atggtgcatt taagcaatct ctagccaacg 2160atgttgaagg
tttattagaa ttatatgaag ccacaagcat gagagtccca ggagagatca
2220tattggaaga tgctctaggg ttcacacgtt ctagattgtc aataatgacc
aaggacgcat 2280tcagtactaa ccctgcgtta ttcactgaaa tccaaagggc
actaaagcaa cctctttgga 2340aaagattgcc tcgtattgaa gcagcacaat
atataccctt ctatcagcag caggactctc 2400acaacaaaac tctacttaaa
ttagcaaagt tagaattcaa tttgttgcaa agtttacata 2460aggaagaatt
atctcacgta tgcaaatggt ggaaagcttt cgatattaag aagaacgccc
2520catgcctgag agacagaata gttgagtgtt atttctgggg tctgggttcc
ggttatgaac 2580cacaatattc tagggccaga gtctttttta ccaaagttgt
cgcggtcata actttaatcg 2640acgatactta tgatgcttat ggtacctacg
aggagttaaa gatattcacc gaagctgtgg 2700aaaggtggtc tataacttgc
ttggacaccc taccagaata tatgaaacca atttacaaat 2760tgtttatgga
tacttatact gaaatggagg aattcctggc aaaagaaggt agaactgatt
2820tgtttaattg cggtaaggaa tttgtgaagg aattcgtaag aaacttaatg
gtcgaggcaa 2880aatgggccaa cgaaggacac attccaacaa ccgaagagca
tgatcctgtc gtgataatca 2940ccggtggcgc taacctacta accactacct
gctacctagg tatgtctgat atcttcacaa 3000aagagagtgt tgaatgggca
gttagtgctc cgcctttgtt caggtatagc ggtatacttg 3060gcaggcgttt
aaatgatctt atgacccata aagccgaaca agaaagaaaa cactcaagct
3120cctctcttga atcctatatg aaggaataca atgttaacga ggaatatgcg
caaactttaa 3180tatacaagga ggtggaagat gtgtggaaag atatcaacag
agaatatctt actacaaaga 3240acataccccg tccacttttg atggcagtga
tttacttgtg tcagttctta gaggtacaat 3300acgctggtaa ggataacttt
acaagaatgg gcgacgaata caaacatttg atcaagtcat 3360tattagttta
tcccatgtcc atctaagcta gctaagatcc gctctaaccg aaaaggaagg
3420agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt
tagtattaag 3480aacgttattt atatttcaaa tttttctttt ttttctgtac
agacgcgtgt acgcatgtaa 3540cattatactg aaaaccttgc ttgagaaggt
tttgggacgc tcgaagcgga ggttgtttat 3600ctttcgagta ctgaatgttg
tcagtatagc tatcctattt gaaactcccc atcgtcttgc 3660tcttgttccc
aatgtttgtt tatacactca tatggctata cccttatcta cttgcctctt
3720ttgtttatgt ctatgtattt gtataaaata tgatattact cagactcaag
caaacaatca 3780attcttagca tcattctttg ttcttatctt aaccataaac
gatcttgatg tgacttttgt 3840aatttgaacg aattggctat acgggacgga
tgacaaatgc accattactc taggttgttg 3900ttggatctta acaaaccgta
aaggtaaact gcccatgcgg ttcacatgac ttttgacttt 3960cctttgtttg
ctagttacct tcggcttcac aatttgtttt tccacttttc taacaggttt
4020atcacctttc aaacttatct ttatcttatt cgccttcttg ggtgcctcca
cagtagaggt 4080tacttccttt ttaatatgta cttttaggat actttcacgc
tttataacaa tatcaagttt 4140accttcttca ttactattca tcttcgccac
aagtcttctc tcccttggtg tttccaatct 4200aactacaaaa ctgttgatta
gggtgtacat caccctaaca agatcatgta tttgcttcct 4260ctggtacaag
ctaagaacag gtaaattcaa aacatcccag agtaatatct tcaaagggct
4320atacccttta aacatatctc ggcatatttg tattaaccca ctaatatttt
gacggccaat 4380cttttctatt tttattttca tatcatcgac gtaatgacca
cttaaaaac
44293123DNAArtificial SequenceSynthetic Primer CUT24 31gtttcttttg
gattgcgctt gcc 233220DNAArtificial SequenceSynthetic Primer ART12
32tactgacaac cacatgttac 203329DNAArtificial SequenceSynthetic
Primer ART45 33tactgcttcg gtagtagttt cacccttca 293420DNAArtificial
SequenceSynthetic Primer ART210 34gggaagtcca attcaatagt
203525DNAArtificial SequenceSynthetic Primer HJ207 35catcttctcg
agataacacc tggag 253619DNAArtificial SequenceSynthetic Primer KB349
36acgcgtgtac gcatgtaac 193720DNAArtificial SequenceSynthetic Primer
HJ602 37caattggggt tctggcagtc 203830DNAArtificial SequenceSynthetic
Primer CUT76 38gaagcctgct ttcaaaatta agaacaaagc 303930DNAArtificial
SequenceSynthetic Primer HJ362 39gaatttacct gttcttagct tgtaccagag
30406523DNAArtificial SequenceSynthetic ADS_HIS3 donor cassette
40attgtgaggg tcagttattt catccagata taacccgaga ggaaacttct tagcgtctgt
60tttcgtacca taaggcagtt catgaggtat attttcgtta ttgaagccca gctcgtgaat
120gcttaatgct gctgaactgg tgtccatgtc gcctaggtac gcaatctcca
caggctgcaa 180aggttttgtc tcaagagcaa tgttattgtg caccccgtaa
ttggtcaaca agtttaatct 240gtgcttgtcc accagctctg tcgtaacctt
cagttcatcg actatctgaa gaaatttact 300aggaatagtg ccatggtaca
gcaaccgaga atggcaattt ctactcgggt tcagcaacgc 360tgcataaacg
ctgttggtgc cgtagacata ttcgaagata ggattatcat tcataagttt
420cagagcaatg tccttattct ggaacttgga tttatggctc ttttggttta
atttcgcctg 480attcttgatc tcctttagct tctcgacgtg ggcctttttc
ttgccatatg gatccgctgc 540acggtcctgt tccctagcat gtacgtgagc
gtatttcctt ttaaaccacg acgctttgtc 600ttcattcaac gtttcccatt
gtttttttct actattgctt tgctgtggga aaaacttatc 660gaaagatgac
gactttttct taattctcgt tttaagagct tggtgagcgc taggagtcac
720tgccaggtat cgtttgaaca cggcattagt cagggaagtc ataacacagt
cctttcccgc 780aattttcttt ttctattact cttggcctcc tctagtacac
tctatatttt tttatgcctc 840ggtaatgatt ttcatttttt ttttttccac
ctagcggatg actctttttt tttcttagcg 900attggcatta tcacataatg
aattatacat tatataaagt aatgtgattt cttcgaagaa 960tatactaaaa
aaatcgttat tgtcttgaag gtgaaatttc tactcttatt aatggtgaac
1020gttaagctga tgctatgatg gaagctgatt ggtcttaact tgcttgtcat
cttgctaatg 1080gtcattggct cgtgttatta cttaagttat ttgtactcgt
tttgaacgta atgctaatga 1140tcatcttatg gaataatagt gagtggtttc
agggtccata aagcttttca attcatcttt 1200tttttttttg ttcttttttt
tgattccggt ttctttgaaa tttttttgat tcggtaatct 1260ccgagcagaa
ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
1320gtggtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
agaacaaaaa 1380cctgcaggaa acgaagataa atcatgtcga aagctacata
taaggaacgt gctgctactc 1440atcctagtcc tgttgctgcc aagctattta
atatcatgca cgaaaagcaa acaaacttgt 1500gtgcttcatt ggatgttcgt
accaccaagg aattactgga gttagttgaa gcattaggtc 1560ccaaaatttg
tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
1620cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
gaagacagaa 1680aatttgctga cattggtaat acagtcaaat tgcagtactc
tgcgggtgta tacagaatag 1740cagaatgggc agacattacg aatgcacacg
gtgtggtggg cccaggtatt gttagcggtt 1800tgaagcaggc ggcggaagaa
gtaacaaagg aacctagagg ccttttgatg ttagcagaat 1860tgtcatgcaa
gggctcccta gctactggag aatatactaa gggtactgtt gacattgcga
1920agagtgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
ggaagagatg 1980aaggttacga ttggttgatt atgacacccg gtgtgggttt
agatgacaag ggagacgcat 2040tgggtcaaca gtatagaacc gtggatgatg
tggtctctac aggatctgac attattattg 2100ttggaagagg actatttgca
aagggaaggg atgctaaggt agagggtgaa cgttacagaa 2160aagcaggctg
ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
2220aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
tatcagttat 2280taccacgaaa atcgttattg tcttgaaggt gaaatttcta
ctcttattaa tggtgaacgt 2340taagctgatg ctatgatgga agctgattgg
tcttaacttg cttgtcatct tgctaatggt 2400catatggctc gtgttattac
ttaagttatt tgtactcgtt ttgaacgtaa tgctaatgat 2460catcttatgc
ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca
2520tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata
acgttcttaa 2580tactaacata actataaaaa aataaatagg gacctagact
tcaggttgtc taactccttc 2640cttttcggtt agagcggatc ttagctagct
tagctagctt agatagacat agggtagacc 2700aacaaagact taattaagtg
cttatattcg tcacccattc tggtgaaatt gtctttacca 2760gcgtattgaa
cttctaagaa ttgacataaa tagataacag ccattaacaa tggacgtgga
2820atatttttgg tggtcaagta ttctctgtta atatctttcc aaacgtcttc
aacttccttg 2880taaatcaaag tttgagcgta ttcttcgtta acgttgtatt
ccttcatgta agattctaag 2940gaagaggagg agtgctttct ttcttgctca
gccttgtgag tcatcaaatc gtttaatctt 3000ctacccaaaa taccagagta
tctgaacaaa ggtggagcgg aaacggccca ttcaacagat 3060tccttggtga
aaatatcaga catacctaag taacaagtag tagtcaacaa gttagcacca
3120ccagtaatga taacgactgg atcgtgttct tcagtagttg gaatatgacc
ttcgttagcc 3180catttagctt caaccatcaa gttacgaacg aattccttaa
cgaattcctt accacaattg 3240aacaagtcgg tacgaccttc cttagccaag
aattcttcca tttcggtgta agtgtccatg 3300aacaacttat aaattggctt
catgtattct ggtaaagtat ctaaacaggt aatggaccat 3360ctttcgacag
cttcagtgaa aatcttcaat tcttcgtagg taccgtaagc gtcgtaagtg
3420tcgtcaatca aggtaataac agcaacagcc ttagtaaaga aaactctagc
tctagagtat 3480tgtggttcgt aaccggaacc caaaccccag aagtaacatt
cgacaattct atctctcaaa 3540catggagcgt tcttcttaat atcgaaggct
ttccaccact tacaaacgtg agacaattct 3600tccttgtgca aggattgcaa
caagttaaat tccaatttag ccaatttcaa caaggttttg 3660ttgtgagagt
cttgttgttg gtagaatggg atgtattgag cagcttcgat tcttggcaaa
3720cgcttccaca atggttgttt caaagctctt tggatttcag tgaacaaagc
tgggttagta 3780gagaaggcat ctttagtcat gatggacaat ctagatctag
taaaacccaa agcgtcttcc 3840aagatgattt caccaggaac tctcatggag
gtggcttcat acaattctaa caaaccttca 3900acatcgttgg ccaaagattg
cttaaaagca ccattcttat ctttgtagtt gttgaaaacg 3960tcacaagtaa
cgtagtaacc ttgctttctc attaatctga accataagga agatctgtca
4020ccgttccagt tatcaccgta agtttcgtaa atacattgca aagcgtgatc
aatttctctt 4080tcgaaatggt atggaatacc taatctttgg atttcgtcga
ttaatttcaa taagttagcg 4140tgcttcattg ggatgtccaa agcttccttc
aacaattgtc taacttcctt cttcaaatcg 4200ttaacgattt gttcaacacc
ttgctcaact tgcttttcgt agatcaagaa ttggtcaccc 4260cagatagaag
gtggaaaatt agcaattggt ctgattggtt tttcttcagt caaagccatg
4320gatccttcag ttacagtaaa tgttttagtt gcatcgtcat aggtccattc
accatcaaca 4380ccattatcat tagcatattg cttaaagacc ttttcggctg
ttgctgcatc tactgcttcg 4440gtagtagttt cacccttcaa agttttacca
ttcaaaatta atttatattg acccattata 4500gttttttctc cttgacgtta
aagtatagag gtatattaac aattttttgt tgatactttt 4560attacatttg
aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
4620tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct
tcgctgatta 4680attaccccag aaataaggct aaaaaactaa tcgcattatc
atcctatggt tgttaatttg 4740attcgttcat ttgaaggttt gtggggccag
gttactgcca atttttcctc ttcataacca 4800taaaagctag tattgtagaa
tctttattgt tcggagcagt gcggcgcgag gcacatctgc 4860gtttcaggaa
cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
4920ctgtcacccg ctcggcggct tctaatccgt acttcaatat agcaatgagc
agttaagcgt 4980attactgaaa gttccaaaga gaaggttttt ttaggctaag
ataatggggc tctttacatt 5040tccacaacat ataagtaaga ttagatatgg
atatgtatat ggatatgtat atggtggtaa 5100tgccatgtaa tatgattatt
aaacttcttt gcgtccatcc aaaaaaaaag taagaatttt 5160tgaaaattca
atataaatgt ctcagaacgt ttacattgta tcgactgcca gaaccccaat
5220tggttcattc cagggttctc tatcctccaa gacagcagtg gaattgggtg
ctgttgcttt 5280aaaaggcgcc ttggctaagg ttccagaatt ggatgcatcc
aaggattttg acgaaattat 5340ttttggtaac gttctttctg ccaatttggg
ccaagctccg gccagacaag ttgctttggc 5400tgccggtttg agtaatcata
tcgttgcaag cacagttaac aaggtctgtg catccgctat 5460gaaggcaatc
attttgggtg ctcaatccat caaatgtggt aatgctgatg ttgtcgtagc
5520tggtggttgt gaatctatga ctaacgcacc atactacatg ccagcagccc
gtgcgggtgc 5580caaatttggc caaactgttc ttgttgatgg tgtcgaaaga
gatgggttga acgatgcgta 5640cgatggtcta gccatgggtg tacacgcaga
aaagtgtgcc cgtgattggg atattactag 5700agaacaacaa gacaattttg
ccatcgaatc ctaccaaaaa tctcaaaaat ctcaaaagga 5760aggtaaattc
gacaatgaaa ttgtacctgt taccattaag ggatttagag gtaagcctga
5820tactcaagtc acgaaggacg aggaacctgc tagattacac gttgaaaaat
tgagatctgc 5880aaggactgtt ttccaaaaag aaaacggtac tgttactgcc
gctaacgctt ctccaatcaa 5940cgatggtgct gcagccgtca tcttggtttc
cgaaaaagtt ttgaaggaaa agaatttgaa 6000gcctttggct attatcaaag
gttggggtga ggccgctcat caaccagctg attttacatg 6060ggctccatct
cttgcagttc caaaggcttt gaaacatgct ggcatcgaag acatcaattc
6120tgttgattac tttgaattca atgaagcctt ttcggttgtc ggtttggtga
acactaagat 6180tttgaagcta gacccatcta aggttaatgt atatggtggt
gctgttgctc taggtcaccc 6240attgggttgt tctggtgcta gagtggttgt
tacactgcta tccatcttac agcaagaagg 6300aggtaagatc ggtgttgccg
ccatttgtaa tggtggtggt ggtgcttcct ctattgtcat 6360tgaaaagata
tgattacgtt ctgcgatttt ctcatgatct ttttcataaa atacataaat
6420atataaatgg ctttatgtat aacaggcata atttaaagtt ttatttgcga
ttcatcgttt 6480ttcaggtact caaacgctga ggtgtgcctt ttgacttact ttt
65234110379DNAArtificial SequenceSynthetic ADS_FS.1 donor cassette
41atattgtttt ttccctagct gttttcggtt tggttatgaa cgcattgtct gcgaaaagaa
60acgtggataa gtattataga aacagcactt cttcggccaa caatatcacg caaattgagc
120aagatagtgc tataaaaggt cttttgccct tttttgccta ttatgcaagc
attgctttac 180tagtgtggat gcaaccaagc tttattacac tctctttcat
cctttccgtt ggtttcacgg 240gagcatttac cgtcggaaga ataatcgttt
gccatttaac taagcagagc tttcccatgt 300tcaatgcacc catgttaatt
cctttgtgcc agatagtatt gtacaaaata tgtctatccc 360tttggggaat
tgagtctaat aaaatcgtct ttgccctatc ttggcttggg ttcggtctct
420cactaggtgt tcacattatg tttatgaatg acattatcca tgaatttact
gagtacctgg 480acgtttatgc tttatccatc aagcgctcca agctgacata
aatcgcactt tgtatctact 540tttttttatt cgaaaacaag gcacaacaat
gaatctatcg ccctgtgaga ttttcaatct 600caagtttgtg taatagatag
cgttatatta tagaactata aaggtccttg aatatacata 660gtgtttcatt
cctattactg tatatgtgac tttacattgt tacttccgcg gctatttgac
720gttttctgct tcaggtgcgg cttggagggc aaagtgtcag aaaatcggcc
aggccgtatg 780acacaaaaga gtagaaaacg agatctcaaa tatctcgagg
cctgtcctct atacaaccgc 840ccagctctct gacaaagctc cagaacggtt
gtcttttgtt tcgaaaagcc aaggtccctt 900ataattgccc tccattttgt
gtcacctatt taagcaaaaa attgaaagtt tactaacctt 960tcattaaaga
gaaataacaa tattataaaa agcgcttaaa tatacctgag aaagcaacct
1020gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct
aagagtcact 1080ttaaaatttg tatacactta ttttttttat aacttattta
ataataaaaa tcataaatca 1140taagaaattc gcttatttag aagtgtcaac
aacgtatcta ccaacgattt gacccttttc 1200catcttttcg taaatttctg
gcaaggtaga caagccgaca accttgattg gagacttgac 1260caaacctctg
gcgaagaatt gttaattaag agctcagatc ttatcgtcgt catccttgta
1320atccatcgat actagtgcgg ccgcccttta gtgagggttg aattcgaatt
ttcaaaaatt 1380cttacttttt ttttggatgg acgcaaagaa gtttaataat
catattacat ggcattacca 1440ccatatacat atccatatac atatccatat
ctaatcttac ttatatgttg tggaaatgta 1500aagagcccca ttatcttagc
ctaaaaaaac cttctctttg gaactttcag taatacgctt 1560aactgctcat
tgcgccgccg agcgggtgac agccctccga aggaagactc tcctccgtgc
1620gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc
gcactgctcc 1680gaacaataaa gattctacaa tactagcttt tatggttatg
aagaggaaaa attggcagta 1740acctggcccc acaaaccttc aaatgaacga
atcaaattaa caaccatagg atgataatgc 1800gattagtttt ttagccttat
ttctggggta attaatcagc gaagcgatga tttttgatct 1860attaacagat
atataaatgc aaaaactgca taaccacttt aactaatact ttcaacattt
1920tcggtttgta ttacttctta ttcaaatgta ataaaagtat caacaaaaaa
ttgttaatat 1980acctctatac tttaacgtca aggagaaaaa actataatgg
gtcaatataa attaattttg 2040aatggtaaaa ctttgaaggg tgaaactact
accgaagcag tagatgcagc aacagccgaa 2100aaggtcttta agcaatatgc
taatgataat ggtgttgatg gtgaatggac ctatgacgat 2160gcaactaaaa
catttactgt aactgaagga tccatggctt tgactgaaga aaaaccaatc
2220agaccaattg ctaattttcc accttctatc tggggtgacc aattcttgat
ctacgaaaag 2280caagttgagc aaggtgttga acaaatcgtt aacgatttga
agaaggaagt tagacaattg 2340ttgaaggaag ctttggacat cccaatgaag
cacgctaact tattgaaatt aatcgacgaa 2400atccaaagat taggtattcc
ataccatttc gaaagagaaa ttgatcacgc tttgcaatgt 2460atttacgaaa
cttacggtga taactggaac ggtgacagat cttccttatg gttcagatta
2520atgagaaagc aaggttacta cgttacttgt gacgttttca acaactacaa
agataagaat 2580ggtgctttta agcaatcttt ggccaacgat gttgaaggtt
tgttagaatt gtatgaagcc 2640acctccatga gagttcctgg tgaaatcatc
ttggaagacg ctttgggttt tactagatct 2700agattgtcca tcatgactaa
agatgccttc tctactaacc cagctttgtt cactgaaatc 2760caaagagctt
tgaaacaacc attgtggaag cgtttgccaa gaatcgaagc tgctcaatac
2820atcccattct accaacaaca agactctcac aacaaaacct tgttgaaatt
ggctaaattg 2880gaatttaact tgttgcaatc cttgcacaag gaagaattgt
ctcacgtttg taagtggtgg 2940aaagccttcg atattaagaa gaacgctcca
tgtttgagag atagaattgt cgaatgttac 3000ttctggggtt tgggttccgg
ttacgaacca caatactcta gagctagagt tttctttact 3060aaggtggttg
ctgttattac cttgattgac gacacttacg acgcttacgg tacctacgaa
3120gaattgaaga ttttcactga agctgtcgaa agatggtcca ttacctgttt
agatacttta 3180ccagaataca tgaagccaat ttataagttg ttcatggaca
cttacaccga aatggaagaa 3240ttcttggcta aggaaggtcg taccgacttg
ttcaattgtg gtaaggaatt cgttaaggaa 3300ttcgttcgta acttgatggt
tgaagctaaa tgggctaacg aaggtcatat tccaactact 3360gaagaacacg
atccagtcgt tatcattact ggtggtgcta acttgttgac tactacttgt
3420tacttaggta tgtctgatat tttcaccaag gaatctgttg aatgggccgt
ttccgctcca 3480cctttgttca gatactctgg tattttgggt agaagattaa
acgatttgat gactcacaag 3540gctgagcaag aaagaaagca ctcctcctct
tccttagaat cttacatgaa ggaatacaac 3600gttaacgaag aatacgctca
aactttgatt tacaaggaag ttgaagacgt ttggaaagat 3660attaacagag
aatacttgac caccaaaaat attccacgtc cattgttaat ggctgttatc
3720tatttatgtc aattcttaga agttcaatac gctggtaaag acaatttcac
cagaatgggt 3780gacgaatata agcacttaat taagtctttg ttggtctacc
ctatgtctat ctaagatccg 3840ctctaaccga aaaggaagga gttagacaac
ctgaagtcta ggtccctatt tattttttta 3900tagttatgtt agtattaaga
acgttattta tatttcaaat ttttcttttt tttctgtaca 3960gacgcgtgta
cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
4020cgaaggtttt caatagttcg gtaatattaa cggataccta ctattatccc
ctagtaggct 4080cttttcacgg agaaattcgg gagtgttttt tttccgtgcg
cattttctta gctatattct 4140tccagcttcg cctgctgccc ggtcatcgtt
cctgtcacgt agtttttccg gattcgtccg 4200gctcatataa taccgcaata
aacacggaat atctcgttcc gcggattcgg ttaaactctc 4260ggtcgcggat
tatcacagag aaagcttcgt ggagaatttt tccagatttt ccgctttccc
4320cgatgttggt atttccggag gtcattatac tgaccgccat tataatgact
gtacaacgac 4380cttctggaga aagaaacaac tcaataacga tgtgggacat
tgggggccca ctcaaaaaat 4440ctggggacta tatccccaga gaatttctcc
agaagagaag aaaagtcaaa gttttttttc 4500gcttgggggt tgcatataaa
tacaggcgct gttttatctt cagcatgaat attccataat 4560tttacttaat
agcttttcat aaataataga atcacaaaca aaatttacat ctgagttaaa
4620caatcatgac aatcaaggaa cataaagtag tttatgaagc tcacaacgta
aaggctctta 4680aggctcctca acatttttac aacagccaac ccggcaaggg
ttacgttact gatatgcaac 4740attatcaaga aatgtatcaa caatctatca
atgagccaga aaaattcttt gataagatgg 4800ctaaggaata cttgcattgg
gatgctccat acaccaaagt tcaatctggt tcattgaaca 4860atggtgatgt
tgcatggttt ttgaacggta aattgaatgc atcatacaat tgtgttgaca
4920gacatgcctt tgctaatccc gacaagccag ctttgatcta tgaagctgat
gacgaatccg 4980acaacaaaat catcacattt ggtgaattac tcagaaaagt
ttcccaaatc gctggtgtct 5040taaaaagctg gggcgttaag aaaggtgaca
cagtggctat ctatttgcca atgattccag 5100aagcggtcat tgctatgttg
gctgtggctc gtattggtgc tattcactct gttgtctttg 5160ctgggttctc
cgctggttcg ttgaaagatc gtgtcgttga cgctaattct aaagtggtca
5220tcacttgtga tgaaggtaaa agaggtggta agaccatcaa cactaaaaaa
attgttgacg 5280aaggtttgaa cggagtcgat ttggtttccc gtatcttggt
tttccaaaga actggtactg 5340aaggtattcc aatgaaggcc ggtagagatt
actggtggca tgaggaggcc gctaagcaga 5400gaacttacct acctcctgtt
tcatgtgacg ctgaagatcc tctattttta ttatacactt 5460ccggttccac
tggttctcca aagggtgtcg ttcacactac aggtggttat ttattaggtg
5520ccgctttaac aactagatac gtttttgata ttcacccaga agatgttctc
ttcactgccg 5580gtgacgtcgg ctggatcacg ggtcacacct atgctctata
tggtccatta accttgggta 5640ccgcctcaat aattttcgaa tccactcctg
cctacccaga ttatggtaga tattggagaa 5700ttatccaacg tcacaaggct
acccatttct atgtggctcc aactgcttta agattaatca 5760aacgtgtagg
tgaagccgaa attgccaaat atgacacttc ctcattacgt gtcttgggtt
5820ccgtcggtga accaatctct ccagacttat gggaatggta tcatgaaaaa
gtgggtaaca 5880aaaactgtgt catttgtgac actatgtggc aaacagagtc
tggttctcat ttaattgctc 5940ctttggcagg tgctgtccca acaaaacctg
gttctgctac cgtgccattc tttggtatta 6000acgcttgtat cattgaccct
gttacaggtg tggaattaga aggtaatgat gtcgaaggtg 6060tccttgccgt
taaatcacca tggccatcaa tggctagatc tgtttggaac caccacgacc
6120gttacatgga tacttacttg aaaccttatc ctggtcacta tttcacaggt
gatggtgctg 6180gtagagatca tgatggttac tactggatca ggggtagagt
tgacgacgtt gtaaatgttt 6240ccggtcatag attatccaca tcagaaattg
aagcatctat ctcaaatcac gaaaacgtct 6300cggaagctgc tgttgtcggt
attccagatg aattgaccgg tcaaaccgtc gttgcatatg 6360tttccctaaa
agatggttat ctacaaaaca acgctactga aggtgatgca gaacacatca
6420caccagataa tttacgtaga gaattgatct tacaagttag gggtgagatt
ggtcctttcg 6480cctcaccaaa aaccattatt ctagttagag atctaccaag
aacaaggtca ggaaagatta 6540tgagaagagt tctaagaaag gttgcttcta
acgaagccga acagctaggt gacctaacta 6600ctttggccaa cccagaagtt
gtacctgcca tcatttctgc tgtagagaac caatttttct 6660ctcaaaaaaa
gaaataaatt gaattgaatt gaaatcgata gatcaatttt tttcttttct
6720ctttccccat cctttacgct aaaataatag tttattttat tttttgaata
ttttttattt 6780atatacgtat atatagacta ttatttatct tttaatgatt
attaagattt ttattaaaaa 6840aaaattcgct cctcttttaa tgcctttatg
cagttttttt ttcccattcg atatttctat 6900gttcgggttc agcgtatttt
aagtttaata actcgaaaat tctgcgttcg ttaaagcttt 6960cgagaaggat
attatttcga aataaaccgt gttgtgtaag cttgaagcct ttttgcgctg
7020ccaatattct tatccatcta ttgtactctt tagatccagt atagtgtatt
cttcctgctc 7080caagctcatc ccatccccgc gtgcttggcc ggccgttttg
ccagcttact atccttcttg 7140aaaatatgca ctctatatct tttagttctt
aattgcaaca catagatttg ctgtataacg 7200aattttatgc tattttttaa
atttggagtt cagtgataaa agtgtcacag cgaatttcct 7260cacatgtagg
gaccgaattg tttacaagtt ctctgtacca ccatggagac atcaaaaatt
7320gaaaatctat ggaaagatat ggacggtagc aacaagaata tagcacgagc
cgcggagttc
7380atttcgttac ttttgatatc actcacaact attgcgaagc gcttcagtga
aaaaatcata 7440aggaaaagtt gtaaatatta ttggtagtat tcgtttggta
aagtagaggg ggtaattttt 7500cccctttatt ttgttcatac attcttaaat
tgctttgcct ctccttttgg aaagctatac 7560ttcggagcac tgttgagcga
aggctcatta gatatatttt ctgtcatttt ccttaaccca 7620aaaataaggg
aaagggtcca aaaagcgctc ggacaactgt tgaccgtgat ccgaaggact
7680ggctatacag tgttcacaaa atagccaagc tgaaaataat gtgtagctat
gttcagttag 7740tttggctagc aaagatataa aagcaggtcg gaaatattta
tgggcattat tatgcagagc 7800atcaacatga taaaaaaaaa cagttgaata
ttccctcaaa aatgtcttac accgtcggaa 7860cctacttggc cgagaggttg
gtccagatcg gattgaagca ccacttcgcc gtcgccggtg 7920actacaactt
ggtcttgttg gacaacttgt tgttgaacaa gaacatggag caggtctatt
7980gctgcaacga gttgaactgc ggtttctcag cagaaggtta tgcaagagcc
aagggagcag 8040ccgctgccgt cgtcacctac tcagtcggtg cattatcagc
attcgatgca attggaggtg 8100cttacgctga gaacttgcca gtcatcttga
tctctggagc acctaacaac aacgaccatg 8160ctgctggtca cgtattgcac
cacgccttgg gtaaaacaga ctaccactac cagttggaaa 8220tggcaaaaaa
tattaccgca gccgcagagg ccatctacac cccagaggaa gcacctgcca
8280aaattgacca cgtcataaag accgctttga gagagaagaa gcctgtttac
ttggagatcg 8340cctgcaacat cgcttctatg ccatgcgccg cacctggtcc
agcctctgct ttgttcaacg 8400acgaggcctc tgacgaagct tcattgaacg
ccgcagtcga agagacatta aagttcatcg 8460ccaacaggga caaagttgcc
gtcttagtcg gttcaaagtt gagggccgct ggtgccgaag 8520aggcagctgt
caagttcgct gacgccttgg gaggagccgt cgccaccatg gccgcagcaa
8580aatctttctt tcctgaggag aacccacatt acatcggaac ctcatggggt
gaagtatcat 8640atcctggagt agaaaaaacc atgaaagagg ccgatgccgt
aatagcattg gctcctgtct 8700tcaacgacta ctcaaccaca ggatggactg
atataccaga tccaaagaaa ttagtcttgg 8760ctgagcctag gtctgtcgtc
gtaaacggta tcaggttccc ttctgttcat ttgaaggact 8820acttaacaag
attggcccaa aaggtatcta aaaagactgg tgccttggac ttcttcaagt
8880cattaaacgc aggagaattg aaaaaagcag caccagccga tccatcagcc
ccattagtta 8940acgctgaaat cgctagacaa gtagaggctt tgttgactcc
aaacactacc gtcatagctg 9000agacaggtga ctcttggttc aacgcacaga
gaatgaaatt gccaaatggt gccagggtcg 9060agtatgaaat gcagtgggga
catataggtt ggtcagtccc agccgccttt ggatacgcag 9120taggtgcccc
tgagaggagg aacatattga tggttggtga tggttcattc caattaacag
9180cccaggaggt agcccaaatg gtcaggttga agttgcctgt catcatcttc
ttgatcaaca 9240attacggata caccatcgag gtcatgatcc acgacggacc
ttacaacaac atcaaaaact 9300gggactacgc cggtttgatg gaggttttca
acggtaacgg tggttatgac tcaggagccg 9360gtaagggatt aaaggctaag
accggtggtg aattggctga agcaattaag gtcgcattgg 9420ccaacaccga
tggacctaca ttgattgaat gcttcatcgg aagggaggac tgcaccgagg
9480aattggttaa atggggtaaa agggtagccg ctgctaattc aagaaaacca
gttaataaat 9540tattataata agtgaattta ctttaaatct tgcatttaaa
taaattttct ttttatagct 9600ttatgactta gtttcaattt atatactatt
ttaatgacat tttcgattca ttgattgaaa 9660gctttgtgtt ttttcttgat
gcgctattgc attgttcttg tctttttcgc cacatgtaat 9720atctgtagta
gatacctgat acattgtgga tgctgagtga aattttagtt aataatggag
9780gcgctcttaa taattttggg gatattggct taacctgcag gccgcgagcg
ccgatataaa 9840ctaatgattt taaatcgtta aaaaaatatg cgaattctgt
ggatcgaaca caggacctcc 9900agataacttg accgaagttt tttcttcagt
ctggcgctct cccaactgag ctaaatccgc 9960ttactatttg ttatcagttc
ccttcatatc tacatagaat aggttaagta ttttattagt 10020tgccagaaga
actactgata gttgggaata tttggtgaat aatgaagatt gggtgaataa
10080tttgataatt ttgagattca attgttaatc aatgttacaa tattatgtat
acagagtata 10140ctagaagttc tcttcggaga tcttgaagtt cacaaaaggg
aatcgatatt tctacataat 10200attatcatta cttcttcccc atcttatatt
tgtcattcat tattgattat gatcaatgca 10260ataatgattg gtagttgcca
aacatttaat acgatcctct gtaatatttc tatgaataat 10320tatcacagca
acgttcaatt atcttcaatt ccggtgttta aaccccagcg cctggcggg
10379424140DNAArtificial SequenceSynthetic ADS_FS.2 donor cassette
42tcctcttgtg gtccgtggct tgtaattttc gggaaagata gaaatcactg cattcatcga
60aagaagtgaa aataaattgt atcggggaag taatgagggg tggaatacgt aattgcttct
120caatttgata atacaagtta cagtcttttt attgagagtc cctggaagga
aaattattga 180tgtttacctt tttttgcgac gcgcgtctgg ccataaataa
aagggttctc ttgccaagaa 240aaaataaaaa ggcgccttaa gggaccttct
atgacaaata tggtgaggta tgcaacctca 300atgaagagca atgagaaaat
ttaaggggta agtaagcatc ggaatttgtt gtttcctaac 360aatttgtcta
atttactcaa taatatcagg agaattgatc gaaaaaagca aaccaggaac
420ccctcacaaa taagggaaca taaagtaatt gctcgtcttt acatacatgg
cactcaatcc 480cagacgtcgc gtgctaaaaa tccttatatt attggcccct
caggagttta tttgaatttt 540gattgcattg ctttcagtgg acagtatatc
ataaaatttg caagggcata gtgcctgccc 600tacgatgttg taaaacaatt
tctgaaaata ggttcagaat caaaaatgat gtataaatat 660tgaaataaat
tttcacataa attgtgctcc tccgcaaagt cttgactaaa taaacaattt
720gttaatatcc tttcaaaaat tcttactttt tttttggatg gacgcaaaga
agtttaataa 780tcatattaca tggcattacc accatataca tatccatata
catatccata tctaatctta 840cttatatgtt gtggaaatgt aaagagcccc
attatcttag cctaaaaaaa ccttctcttt 900ggaactttca gtaatacgct
taactgctca ttgctatatt gaagtacgga ttagaagccg 960ccgagcgggt
gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg
1020tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat
aaagattcta 1080caatactagc ttttatggtt atgaagagga aaaattggca
gtaacctggc cccacaaacc 1140ttcaaatgaa cgaatcaaat taacaaccat
aggatgataa tgcgattagt tttttagcct 1200tatttctggg gtaattaatc
agcgaagcga tgatttttga tctattaaca gatatataaa 1260tgcaaaaact
gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc
1320ttattcaaat gtaataaaag tatcaacaaa aaattgttaa tatacctcta
tactttaacg 1380tcaaggagaa aaaaccccgg atccatgggt caatataaat
taattttgaa tggtaaaact 1440ttgaagggtg aaactactac cgaagcagta
gatgcagcaa cagccgaaaa ggtctttaag 1500caatatgcta atgataatgg
tgttgatggt gaatggacct atgacgatgc aactaaaaca 1560tttactgtaa
ctgaaggatc catggctttg actgaagaaa aaccaatcag accaattgct
1620aattttccac cttctatctg gggtgaccaa ttcttgatct acgaaaagca
agttgagcaa 1680ggtgttgaac aaatcgttaa cgatttgaag aaggaagtta
gacaattgtt gaaggaagct 1740ttggacatcc caatgaagca cgctaactta
ttgaaattaa tcgacgaaat ccaaagatta 1800ggtattccat accatttcga
aagagaaatt gatcacgctt tgcaatgtat ttacgaaact 1860tacggtgata
actggaacgg tgacagatct tccttatggt tcagattaat gagaaagcaa
1920ggttactacg ttacttgtga cgttttcaac aactacaaag ataagaatgg
tgcttttaag 1980caatctttgg ccaacgatgt tgaaggtttg ttagaattgt
atgaagccac ctccatgaga 2040gttcctggtg aaatcatctt ggaagacgct
ttgggtttta ctagatctag attgtccatc 2100atgactaaag atgccttctc
tactaaccca gctttgttca ctgaaatcca aagagctttg 2160aaacaaccat
tgtggaagcg tttgccaaga atcgaagctg ctcaatacat cccattctac
2220caacaacaag actctcacaa caaaaccttg ttgaaattgg ctaaattgga
atttaacttg 2280ttgcaatcct tgcacaagga agaattgtct cacgtttgta
agtggtggaa agccttcgat 2340attaagaaga acgctccatg tttgagagat
agaattgtcg aatgttactt ctggggtttg 2400ggttccggtt acgaaccaca
atactctaga gctagagttt tctttactaa ggtggttgct 2460gttattacct
tgattgacga cacttacgac gcttacggta cctacgaaga attgaagatt
2520ttcactgaag ctgtcgaaag atggtccatt acctgtttag atactttacc
agaatacatg 2580aagccaattt ataagttgtt catggacact tacaccgaaa
tggaagaatt cttggctaag 2640gaaggtcgta ccgacttgtt caattgtggt
aaggaattcg ttaaggaatt cgttcgtaac 2700ttgatggttg aagctaaatg
ggctaacgaa ggtcatattc caactactga agaacacgat 2760ccagtcgtta
tcattactgg tggtgctaac ttgttgacta ctacttgtta cttaggtatg
2820tctgatattt tcaccaagga atctgttgaa tgggccgttt ccgctccacc
tttgttcaga 2880tactctggta ttttgggtag aagattaaac gatttgatga
ctcacaaggc tgagcaagaa 2940agaaagcact cctcctcttc cttagaatct
tacatgaagg aatacaacgt taacgaagaa 3000tacgctcaaa ctttgattta
caaggaagtt gaagacgttt ggaaagatat taacagagaa 3060tacttgacca
ccaaaaatat tccacgtcca ttgttaatgg ctgttatcta tttatgtcaa
3120ttcttagaag ttcaatacgc tggtaaagac aatttcacca gaatgggtga
cgaatataag 3180cacttaatta agtctttgtt ggtctaccct atgtctatct
aagatccgct ctaaccgaaa 3240aggaaggagt cagacaacct gaagtctagg
tccctattta tttttttata gttatgttag 3300tattaagaac gttatttata
tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg 3360catgtaacat
tatactgaaa accttgcttg agaaggtttt gggacgctcg aagctacctt
3420tttgttcttt tacttaaaca ttagttagtt cgttttcttt ttctcatttt
tttatgtttc 3480ccccccaaag ttctgatttt ataatatttt atttcacaca
attccattta acagaggggg 3540aatagattct ttagcttaga aaattagtga
tcaatatata tttgcctttc ttttcatctt 3600ttcagtgata ttaatggttt
cgagacactg caatggccct agttgtctaa gaggatagat 3660gttactgtca
aagatgatat tttgaatttc aattgacgta attaatgata ctattaataa
3720tacagagcgt atatgaagta ttgcaaataa catgcacagt tcttttggga
tgagaatgat 3780aatgaaaggc gaaggcgggc gttcagaaaa gcgttgcgga
gtaacaagtg attaaatagc 3840acccaaataa tcttctttga tactaccgat
tgcgtgaata gaactcactt gactgataca 3900accttcaatt ttaactctaa
ttctactttt tatggtgatg acatcctcgg aactttggta 3960tgatggtggg
tttgaacccg cattaaaggt taaatcttga ggcatcagat gctttgtcac
4020aaatactttc attggaccta cttgcacttc gaacccgtgc tgagaacatg
aaacgactgt 4080gccgtccact acttcccctt taaatggttt gaaaactaca
gctctatatt tcacgttgaa 4140434475DNAArtificial SequenceSynthetic
ADS_FS.3 donor cassette 43attgaagcac ctgtggagta tttaaaaact
gcggttacat ggcctacaga tgaaatatgt 60gctcaactaa tgacacaatt cccaccagga
acgccgacca gtgtcctgct gcagactatt 120tcagatgagc tagagaaaag
ttctgacaac ctgttcacgt tatctgattt aaagagcaaa 180ctgaaagtta
ttggcttatt cgagcacatg gaagatatcc catttttcga caagctgaaa
240ctaagcaatg cgcccgtgaa ggacatgcct atggtcacaa aggcgttcac
caaattttgc 300gaaacaatag caaaaaggca tacaagaggc ctactgtcat
accgattacc ttttaaccta 360ctggactaca attgcatacc gaatgagagt
tattcattag aggtttatga gtcattgtac 420aacatcatta ctctatactt
ctggctcagc aacaggtacc caaactactt cattgacatg 480gaatctgcta
aagatttgaa gtatttctgt gagatgatta ttttcgagaa acttgatcga
540ttaaagaaga atccttacgc acataagccc tttggttcta caagaggtca
cctctcatct 600tcgagaagaa gattgcgtac ataatctacg atatatcctg
taaatagaaa cagctacact 660gcttgaaagc cttaacatga tacatttctg
gtatgatgcc attgttgtgc cctgccgggt 720ttatcgtttc ctaacaggca
cgtcacttat aacgaggtgc ctgtcgttta ccgcccaagc 780cggttttttc
gctggagagt acggtactac tagcccacca cacgttcgtg gccaggttga
840taggccaccg ttgagcaaag ggcagtaaaa tatataaaag aggaacaagc
gcttccatta 900agagcactgc taagcctact cgttttctag ttctctgaaa
aaaggtagcc taaaacaagc 960gccatatcat atatatttat acagattaga
cgtactcaaa attcttactt tttttttgga 1020tggacgcaaa gaagtttaat
aatcatatta catggcatta ccaccatata catatccata 1080tacatatcca
tatctaatct tacttatatg ttgtggaaat gtaaagagcc ccattatctt
1140agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct
cattgcgccg 1200ccgagcgggt gacagccctc cgaaggaaga ctctcctccg
tgcgtcctcg tcttcaccgg 1260tcgcgttcct gaaacgcaga tgtgcctcgc
gccgcactgc tccgaacaat aaagattcta 1320caatactagc ttttatggtt
atgaagagga aaaattggca gtaacctggc cccacaaacc 1380ttcaaatgaa
cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct
1440tatttctggg gtaattaatc agcgaagcga tgatttttga tctattaaca
gatatataaa 1500tgcaaaaact gcataaccac tttaactaat actttcaaca
ttttcggttt gtattacttc 1560ttattcaaat gtaataaaag tatcaacaaa
aaattgttaa tatacctcta tactttaacg 1620tcaaggagaa aaaactataa
tgggtcaata taaattaatt ttgaatggta aaactttgaa 1680gggtgaaact
actaccgaag cagtagatgc agcaacagcc gaaaaggtct ttaagcaata
1740tgctaatgat aatggtgttg atggtgaatg gacctatgac gatgcaacta
aaacatttac 1800tgtaactgaa ggatccatgg ctttgactga agaaaaacca
atcagaccaa ttgctaattt 1860tccaccttct atctggggtg accaattctt
gatctacgaa aagcaagttg agcaaggtgt 1920tgaacaaatc gttaacgatt
tgaagaagga agttagacaa ttgttgaagg aagctttgga 1980catcccaatg
aagcacgcta acttattgaa attaatcgac gaaatccaaa gattaggtat
2040tccataccat ttcgaaagag aaattgatca cgctttgcaa tgtatttacg
aaacttacgg 2100tgataactgg aacggtgaca gatcttcctt atggttcaga
ttaatgagaa agcaaggtta 2160ctacgttact tgtgacgttt tcaacaacta
caaagataag aatggtgctt ttaagcaatc 2220tttggccaac gatgttgaag
gtttgttaga attgtatgaa gccacctcca tgagagttcc 2280tggtgaaatc
atcttggaag acgctttggg ttttactaga tctagattgt ccatcatgac
2340taaagatgcc ttctctacta acccagcttt gttcactgaa atccaaagag
ctttgaaaca 2400accattgtgg aagcgtttgc caagaatcga agctgctcaa
tacatcccat tctaccaaca 2460acaagactct cacaacaaaa ccttgttgaa
attggctaaa ttggaattta acttgttgca 2520atccttgcac aaggaagaat
tgtctcacgt ttgtaagtgg tggaaagcct tcgatattaa 2580gaagaacgct
ccatgtttga gagatagaat tgtcgaatgt tacttctggg gtttgggttc
2640cggttacgaa ccacaatact ctagagctag agttttcttt actaaggtgg
ttgctgttat 2700taccttgatt gacgacactt acgacgctta cggtacctac
gaagaattga agattttcac 2760tgaagctgtc gaaagatggt ccattacctg
tttagatact ttaccagaat acatgaagcc 2820aatttataag ttgttcatgg
acacttacac cgaaatggaa gaattcttgg ctaaggaagg 2880tcgtaccgac
ttgttcaatt gtggtaagga attcgttaag gaattcgttc gtaacttgat
2940ggttgaagct aaatgggcta acgaaggtca tattccaact actgaagaac
acgatccagt 3000cgttatcatt actggtggtg ctaacttgtt gactactact
tgttacttag gtatgtctga 3060tattttcacc aaggaatctg ttgaatgggc
cgtttccgct ccacctttgt tcagatactc 3120tggtattttg ggtagaagat
taaacgattt gatgactcac aaggctgagc aagaaagaaa 3180gcactcctcc
tcttccttag aatcttacat gaaggaatac aacgttaacg aagaatacgc
3240tcaaactttg atttacaagg aagttgaaga cgtttggaaa gatattaaca
gagaatactt 3300gaccaccaaa aatattccac gtccattgtt aatggctgtt
atctatttat gtcaattctt 3360agaagttcaa tacgctggta aagacaattt
caccagaatg ggtgacgaat ataagcactt 3420aattaagtct ttgttggtct
accctatgtc tatctaagat ccgctctaac cgaaaaggaa 3480ggagttagac
aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
3540agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt
gtacgcatgt 3600aacattatac tgaaaacctt gcttgagaag gttttgggac
gctcgaagga tacttgcaca 3660agttccacta attactgaca tttgtggtat
taactcgttt gactgctcta caattgtagg 3720atgttaatca atgtcttggc
tgccttcatt ctcttcaggc tctattaatt ttaaccgtta 3780taagttcctt
ttctcccttg gaagcaaaca tcaactgcct taaaatctgg tggcgaggaa
3840agaggaaatg gcatgtacta atgatggtcc taataaatat cccgaaattg
tgagtgttaa 3900gcacctgttc caacattcgg gatccaagca tgaatttagt
gctggtaaac gattttcaaa 3960atccattggt aaaatattca aacgaaactc
tgctttgaaa acttctagaa ctgaaacggc 4020aaatcataaa atggaattga
aaaaaagaga gggtgttacc ttattgccac ctgtcccaga 4080atcattatta
cataaactca attcttggtt ggaaactttt tcttccacca agaacatgaa
4140aatcgaagaa aacaaaattg ttattaatga aaaagagatt cgggattcag
tctcttacta 4200ccctgataag aatggaggaa gtgctgtatt ttgttacttg
cccgaccttg tgctatatta 4260taagccgcct ataaaagtca caggcaagca
atgtccaata aagagaagtc cttgggaatc 4320gatggaaatc caatatcaaa
agtttatgta ccccttagaa aggttggaaa gacagtttga 4380ggaagttcca
tttaggccct ggtattttgc aatgcgatta aaggaacttt acagatgctg
4440tgaaaggtct tttactaacg cggcaaatag aggaa 4475448247DNAArtificial
SequenceSynthetic 9XFS_URA3_FS.4 donor cassette 44attgctattg
agtaagttcg atccgtttgg cgtcttttgg ggtgtaacgc caaacttatt 60acttttccta
tttgaggttg gtattgattg ttgtcaaaga atgaaaatat acacaaacgc
120cacaatatac gtaccaggtt cacgaaaact gatcgtatgg ttcataccct
gacttggcaa 180acctaatgtg accgtcgctg attagcggat cacgaaaagt
gatctcgata caattagagg 240atccacgaaa atgatgtgaa tgaatacatg
aaagattcat gagatctgac aacatggtag 300acgtgtgtgt ctcatggaaa
ttgatgcagt tgaagacatg tgcgtcacga aaaaagaaat 360caatcctaca
cagggcttaa gggcaaatgt attcatgtgt gtcacgaaaa gtgatgtaac
420taaatacacg attaccatgg aaattaacgt accttttttg tgcgtgtatt
gaaatattat 480gacatattac agaaagggtt cgcaagtcct gtttctatgc
ctttctctta gtaattcacg 540aaataaacct atggtttacg aaatgatcca
cgaaaatcat gttattattt acatcaacat 600atcgcgaaaa ttcatgtcat
gtccacatta acatcattgc agagcaacaa ttcattttca 660tagagaaatt
tgctactatc acccactagt actaccattg gtacctacta ctttgaattg
720tactaccgct gggcgttatt aggtgtgaaa ccacgaaaag ttcaccataa
cttcgaataa 780agtcgcggaa aaaagtaaac agctattgct actcaaatga
ggtttgcaga agcttgttga 840agcatgatga agcgttctaa acgcactatt
catcattaaa tatttaaagc tcataaaatt 900gtattcaatt cctattctaa
atggctttta tttctattac aactattagc tctaaatcca 960tatcctcata
agcagcaatc aattctatct atactttaaa agtaaaattc ttgagggaac
1020tttcaccatt atgggaaatg gttcaagaag gtattgactt aaactccatc
aaatggtcag 1080gtcattgagt gttttttatt tgttgtattt tttttttttt
agagaaaatc ctccaatatc 1140aaattaggaa tcgtagtttc atgattttct
gttacaccta actttttgtg tggtgccctc 1200ctccttgtca atattaatgt
taaagtgcaa ttctttttcc ttatcacgtt gagccattag 1260tatcaatttg
cttacctgta ttcctttact atcctccttt ttctccttct tgataaatgt
1320atgtagattg cgtatatagt ttcgtctacc ctatgaacat attccatttt
gtaatttcgt 1380gtcgtttcta ttatgaattt catttataaa gtttatgtac
aaatatcata aaaaaagaga 1440atctttttaa gcaaggattt tcttaacttc
ttcggcgaca gcatcaccga cttcggtggt 1500actgttggaa ccacctaaat
caccagttct gatacctgca tccaaaacct ttttaactgc 1560atcttcaatg
gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc
1620agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag
cagaaccgtg 1680gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc
aaagaggcca aggacgcaga 1740tggcaacaaa cccaaggaac ctgggataac
ggaggcttca tcggagatga tatcaccaaa 1800catgttgctg gtgattataa
taccatttag gtgggttggg ttcttaacta ggatcatggc 1860ggcagaatca
atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc
1920ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat
ccaaggacca 1980aataggcaat ggtggctcat gttgtagggc catgaaagcg
gccattcttg tgattctttg 2040cacttctgga acggtgtatt gttcactatc
ccaagcgaca ccatcaccat cgtcttcctt 2100tctcttacca aagtaaatac
ctcccactaa ttctctgaca acaacgaagt cagtaccttt 2160agcaaattgt
ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg
2220tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt
gttcaggtct 2280aacactaccg gtaccccatt taggaccacc cacagcacct
aacaaaacgg catcaacctt 2340cttggaggct tccagcgcct catctggaag
tgggacacct gtagcatcga tagcagcacc 2400accaattaaa tgattttcga
aatcgaactt gacattggaa cgaacatcag aaatagcttt 2460aagaacctta
atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac
2520gatcttctta ggggcagaca taggggcaga cattagaatg gtatatcctt
gaaatatata 2580tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
gtaagacgat tgctaaccac 2640ctattggaaa aaacaatagg tccttaaata
atattgtcaa cttcaagtat tgtgatgcaa 2700gcatttagtc atgaacgctt
ctctattcta tatgaaaagc cggttccggc ctctcacctt 2760tcctttttct
cccaattttt cagttgaaaa aggtatatgc gtcaggcgac ctctgaaatt
2820aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
tgtgttctcg 2880ttatgttgag gaaaaaaata atggttgcta agagattcga
actcttgcat cttacgatac 2940ctgagtattc ccacagttaa ctgcggtcaa
gatatttctt gaatcaggcg ccttagaccg 3000ctcggccaaa caaccaatta
cttgttgaga aatagagtat aattatccta taaatataac 3060gtttttgaac
acacatgaac aaggaagtac aggacaattg attttgaaga gaatgtggat
3120tttgatgtaa ttgttgggat tccattttta ataaggcaat aatattaggt
atgtggatat 3180actagaagtt ctcctcgacc gtaataatca tattacatgg
cattaccacc atatacatat
3240ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa
gagccccatt 3300atcttagcct aaaaaaacct tctctttgga actttcagta
atacgcttaa ctgctcattg 3360cgccgccgag cgggtgacag ccctccgaag
gaagactctc ctccgtgcgt cctcgtcttc 3420accggtcgcg ttcctgaaac
gcagatgtgc ctcgcgccgc actgctccga acaataaaga 3480ttctacaata
ctagctttta tggttatgaa gaggaaaaat tggcagtaac ctggccccac
3540aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
ttagtttttt 3600agccttattt ctggggtaat taatcagcga agcgatgatt
tttgatctat taacagatat 3660ataaatgcaa aaactgcata accactttaa
ctaatacttt caacattttc ggtttgtatt 3720acttcttatt caaatgtaat
aaaagtatca acaaaaaatt gttaatatac ctctatactt 3780taacgtcaag
gagaaaaaac tataatgtcc actttgccta tttcctctgt ttcttcctct
3840ccatctactt ccccaattgt tgtcgatgat aaggattcta ccaagccaga
cgttattaga 3900cataccgcta acttcaacgc ttccatttgg ggtgatcaat
ttttgactta cgatgaacca 3960gaggacttgg ttatgaagaa gcaattagtc
gaagagttaa aggaggaagt taagaaggaa 4020ttgattacta ttaagggttc
taacgaacct atgcaacatg ttaagttgat cgaattaatc 4080aatgctgttc
aacgtttagg tattgcttac cattttgaag aagaaatcga agaagcttta
4140caacatatcc atgtcactta cggtgaacaa tgggtcgata aggaaaattt
acaatctatc 4200tccttgtggt ttcgtttgtt gcgtcaacaa ggtttcaatg
tctcctctgg tgttttcaag 4260gactttatgg acgaaaaagg taaatttaag
gaatctttgt gtaacgacgc tcaaggtatc 4320ttggccttgt acgaagctgc
tttcatgaga gtcgaagatg aaaccatctt ggacaacgct 4380ttggaattct
ctaaggttca cttggacatt attgccaagg atccatcttg tgactcttcc
4440ttaagaaccc aaatccacca agccttgaag caaccattga gaagaagatt
ggccagaatc 4500gaagctttac actatatgcc aatttaccaa caagaaactt
ctcacgacga ggttttgttg 4560aagttggcta agttagattt ctctgttttg
caatccatgc ataagaaaga attgtctcac 4620atctgcaagt ggtggaaaga
tttagacttg caaaacaaat tgccattcgt tcgtgataga 4680gttgtcgaag
gttacttctg gattttgtct atttactatg aaccacaaca tgccagaacc
4740agaatgttct tgatgaagtc ttgtatgtgg ttggtcgttt tggatgatac
cttcgacaac 4800tacggtacct acgaagaatt ggaaattttc actcaagccg
ttgagaagtg gtccatttct 4860tgtttggaca tgttgccaga atacatgaag
ttgatctacc aagaattggt taacttgcac 4920gttgaaatgg aagaatcttt
agaaaaggaa ggtaaggctt atcaaatcca ctacgttaag 4980gaaatggcta
aggaattggt cagaaactac ttggttgaag ccagatggtt gaaagaaggt
5040tacatgccta ctttggaaga gtacatgtct gtttccatgg ttaccggtac
ctacggtttg 5100atcactgcta gatcttacgt tggtagaggt gacattgtta
acgaggacac ttttaaatgg 5160gtttcttcct acccacctat tgttgaagct
tcttgtgtta tcattagatt gatggatgat 5220attgtctctc ataaagaaga
acaagaaaga ggtcatgttg cctcctccat cgaatgttat 5280tctaaggaat
ccggtgcttc tgaagaagaa gcctgtgaat acatctctag aaaagtcgaa
5340gacgcctgga aggttattaa cagagaatct ttgagaccaa ccgccgttcc
attccctttg 5400ttaatgccag ctatcaactt ggctagaatg tgtgaagttt
tatactctgt taacgatggt 5460ttcactcacg ccgaaggtga catgaaatct
tacatgaagt ccttctttgt ccatccaatg 5520gtcgtttgag cgaatttctt
atgatttatg atttttatta ttaaataagt tataaaaaaa 5580ataagtgtat
acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
5640agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
cgctcatgcg 5700tccatcttta cagtcctgtc ttattgttct tgatttgtgc
cccgtaaaat actgttactt 5760ggttctggcg aggtattgga tagttccttt
ttataaaggc catgaagctt tttctttcca 5820attttttttt tttcgtcatt
atagaaatca ttacgaccga gattcccggg taataactga 5880tataattaaa
ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca
5940gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
ttttctgtaa 6000cgttcaccct ctaccttagc atcccttccc tttgcaaata
gtcctcttcc aacaataata 6060atgtcagatc ctgtagagac cacatcatcc
acggttctat actgttgacc caatgcgtct 6120cccttgtcat ctaaacccac
accgggtgtc ataatcaacc aatcgtaacc ttcatctctt 6180ccacccatgt
ctctttgagc aataaagccg ataacaaaat ctttgtcact cttcgcaatg
6240tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga
caattctgct 6300aacatcaaaa ggcctctagg ttcctttgtt acttcttctg
ccgcctgctt caaaccgcta 6360acaatacctg ggcccaccac accgtgtgca
ttcgtaatgt ctgcccattc tgctattctg 6420tatacacccg cagagtactg
caatttgact gtattaccaa tgtcagcaaa ttttctgtct 6480tcgaagagta
aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc
6540atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt
gggacctaat 6600gcttcaacta actccagtaa ttccttggtg gtacgaacat
ccaatgaagc acacaagttt 6660gtttgctttt cgtgcatgat attaaatagc
ttggcagcaa caggactagg atgagtagca 6720gcacgttcct tatatgtagc
tttcgacatg atttatcttc gtttcctgca ggtttttgtt 6780ctgtgcagtt
gggttaagaa tactgggcaa tttcatgttt cttcaacacc acatatgcgt
6840atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgctc
ggagattacc 6900gaatcaaaaa aatttcaaag aaaccggaat caaaaaaaag
aacaaaaaaa aaaaagatga 6960attgaaaagc tttatggacc ctgaaaccac
agccacatta accttctttg atggtcaaaa 7020cttatccttc accataaata
tgcctcgcaa aaaaggtaat taacatatat agaattacat 7080tatttatgaa
atatcatcac tatctcttag catctttaat ccttttctac atcagataac
7140ttcggtttgt tatcatcgtc tgtattgtca tcaattggcg cagtagcctc
aatttcaacg 7200tcgtttgact ctggtgtttg ttcatgtgca gatccatgag
atgatgaaat gtgtatatta 7260gtttaaaaag ttgtatgtaa taaaagtaaa
atttaatatt ttggatgaaa aaaaccattt 7320ttagactttt tcttaactag
aatgctggag tagaaatacg ccatctcaag atacaaaaag 7380cgttaccggc
actgatttgt ttcaaccagt atatagatta ttattgggtc ttgatcaact
7440ttcctcagac atatcagtaa cagttatcaa gctaaatatt tacgcgaaag
aaaaacaaat 7500attttaattg tgatacttgt gaattttatt ttattaagga
tacaaagtta agagaaaaca 7560aaatttatat acaatataag taatattcat
atatatgtga tgaatgcagt cttaacgaga 7620agacatggcc ttggtgacaa
ctctcttcaa accaacttca gcctttctca attcatcagc 7680agatgggtct
tcgatttgca aagcagccaa agcatcggac aaagcagctt caatcttgga
7740cttggaacct ctcttcaatt tagaagacaa gactgggtca gtgacagttt
gttcgatgga 7800ggcaacgtag gattccaatc tttgtctagc ttcgtgcttc
ttggcaaaag cttcatcggc 7860agccttgaac tcttcagctt ggttaaccat
cttttcaatt tcttcagaag acaatctacc 7920aacagcgtta gagatagtga
tgttagaaga cttaccggta gacttttcga cggcagtaac 7980cttcaagata
ccgttagcat caacttcgaa gatagcttcc aagactggtt caccagctgg
8040catcattggg atgttcttca agtcgaattc acccaacaaa gtgttttctt
tacagttaac 8100acgttcacct tggtagactg ggaattgaac ggtggtttgg
ttgtcagcac atgtagtaaa 8160ggttcttctc ttgatggttg gaacagtagt
gtttcttgga acaacgatac cgaacatgtc 8220accttgcata ccaacaccta gagataa
82474549DNAArtificial SequenceSynthetic Recognition sequence for
FS-specific TALEN 45tagtggagga attaaaagag gaagttaaga aggaattgat
aactatcaa 494626DNAArtificial SequenceSynthetic Primer HJ272
46ataacaatat tataaaaagc gcttaa 264729DNAArtificial
SequenceSynthetic Primer ART45 47tactgcttcg gtagtagttt cacccttca
294822DNAArtificial SequenceSynthetic Primer HJ643 48aaaatcctta
tattattggc cc 224919DNAArtificial SequenceSynthetic Primer HJ799
49gtagcctaaa acaagcgcc 19509PRTArtificial SequenceSynthetic Homing
endonuclease family conserved motif 50Leu Ala Gly Leu Ile Asp Ala
Asp Gly 1 5 516PRTArtificial SequenceSynthetic Homing endonuclease
family conserved motif 51Gly Ile Tyr Tyr Ile Gly 1 5
5218DNAArtificial SequenceSynthetic Recognition sequence for I-SceI
52tagggataac agggtaat 185331DNAArtificial SequenceSynthetic
Recognition sequence for VDE (PI-SceI) 53tatgtcgggt gcggagaaag
aggtaatgaa a 315424DNAArtificial SequenceSynthetic Recognition
sequence for F-CphI 54gatgcacgag cgcaacgctc acaa
245524DNAArtificial SequenceSynthetic Recognition sequence for
PI-MgaI 55gcgtagctgc ccagtatgag tcag 245640DNAArtificial
SequenceSynthetic Recognition sequence for PI-MtuII 56acgtgcacta
cgtagagggt cgcaccgcac cgatctacaa 40574300DNAArtificial
SequenceSynthetic ADE2_SFC1 donor cassette 57ccggtggtgc ttatactgtt
tctactgcag ctgccgctac tgttagatct accatcagaa 60gattaagaga aatggttgaa
gcttaaactt ctttcattca ttttctcttg gctttcacta 120ggtatatcta
ttccataacg actatgtttt gtatttgtta atttacataa aaccatatca
180gtacatcaac gaactgtaaa aaagaaactt tagcataatt attgcggata
tttaaactca 240cttgcaggta agagcaaaag gcgattgatt tccagtccgc
ctcttgtcac gtgatttagt 300aagaaatttt gacagcaccc tcggtttaat
ggaaaagagg ggcgtttttc gatgaacccg 360aggggagact agagaatcat
ctcggttgaa tggagcatta tttttttagt agcgcccgcc 420cggagaaatg
gacgttggcg aatgagccat gaattattaa ccgcccatgt ctaccagata
480gacggcacgg ccacgcgttt aaaccgcccc acatcgtatg acagtaccag
cctagtcccg 540gtaaaccgca aacggacctt aattgtgacg aagggcccaa
atttgatggg tcggtgttaa 600tgattagtcc tcattgtcat aataaagtgt
gatgatggag gcaatgatga tatacggtag 660tactactgct cgaggtgcta
tcttttaacc aatcctttga gattcttgtc gccacggagt 720tactaccttt
tacaaaccgt aatgtcacat tttgcatata tcttatgtat aaatatatag
780ttcacttact acttgttctc gttttgttaa ctttcttgtt gtagttcttc
ttgttcttgg 840cgtttccccc tttgttttct atctgcttca taagtaaagt
gcaaagcatt ttggaagata 900ttatcaattg agtcattgaa agaaacttgg
catcttccct attactaaaa ctaagaatac 960ttgattcaag aaagaagttt
atattagttt tagccgtaag ataacataac aaagaagaag 1020aaagaaaatt
cttgaataat acataacttt tcttaaaaga atcaaagaca gataaaattt
1080aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat
aacactgaca 1140tctttaacaa cttttaatta tgatacattt cttacgtcat
gattgattat tacagctatg 1200ctgacaaatg actcttgttg catggctacg
aaccgggtaa tactaagtga ttgactcttg 1260ctgacctttt attaagaact
aaatggacaa tattatggag catttcatgt ataaattggt 1320gcgtaaaatc
gttggatctc tcttctaagt acatcctact ataacaatca agaaaaacaa
1380gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt
aggaggggga 1440caattgggac gtatgattgt tgaggcagca aacaggctca
acattaagac ggtaatacta 1500gatgctgaaa attctcctgc caaacaaata
agcaactcca atgaccacgt taatggctcc 1560ttttccaatc ctcttgatat
cgaaaaacta gctgaaaaat gtgatgtgct aacgattgag 1620attgagcatg
ttgatgttcc tacactaaag aatcttcaag taaaacatcc caaattaaaa
1680atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca
aaaagagcat 1740ttaatcaaaa atggtatagc agttacccaa agtgttcctg
tggaacaagc cagtgagacg 1800tccctattga atgttggaag agatttgggt
tttccattcg tcttgaagtc gaggactttg 1860gcatacgatg gaagaggtaa
cttcgttgta aagaataagg aaatgattcc ggaagctttg 1920gaagtactga
aggatcgtcc tttgtacgcc gaaaaatggg caccatttac taaagaatta
1980gcagtcatga ttgtgaggtc tgttaacggt ttagtgtttt cttacccaat
tgtagagact 2040atccacaagg acaatatttg tgacttatgt tatgcgcctg
ctagagttcc ggactccgtt 2100caacttaagg cgaagttgtt ggcagaaaat
gcaatcaaat cttttcccgg ttgtggtata 2160tttggtgtgg aaatgttcta
tttagaaaca ggggaattgc ttattaacga aattgcccca 2220aggcctcaca
actctggaca ttataccatt gatgcttgcg tcacttctca atttgaagct
2280catttgagat caatattgga tttgccaatg ccaaagaatt tcacatcttt
ctccaccatt 2340acaacgaacg ccattatgct aaatgttctt ggagacaaac
atacaaaaga taaagagcta 2400gaaacttgcg aaagagcatt ggcgactcca
ggttcctcag tgtacttata tggaaaagag 2460tctagaccta acagaaaagt
aggtcacata aatattattg cctccagtat ggcggaatgt 2520gaacaaaggc
tgaactacat tacaggtaga actgatattc caatcaaaat ctctgtcgct
2580caaaagttgg acttggaagc aatggtcaaa ccattggttg gaatcatcat
gggatcagac 2640tctgacttgc cggtaatgtc tgccgcatgt gcggttttaa
aagattttgg cgttccattt 2700gaagtgacaa tagtctctgc tcatagaact
ccacatagga tgtcagcata tgctatttcc 2760gcaagcaagc gtggaattaa
aacaattatc gctggagctg gtggggctgc tcacttgcca 2820ggtatggtgg
ctgcaatgac accacttcct gtcatcggtg tgcccgtaaa aggttcttgt
2880ctagatggag tagattcttt acattcaatt gtgcaaatgc ctagaggtgt
tccagtagct 2940accgtcgcta ttaataatag tacgaacgct gcgctgttgg
ctgtcagact gcttggcgct 3000tatgattcaa gttatacaac gaaaatggaa
cagtttttat taaagcaaga agaagaagtt 3060cttgtcaaag cacaaaagtt
agaaactgtc ggttacgaag cttatctaga aaacaagtaa 3120tatataagtt
tattgatata cttgtacagc aaataattat aaaatgatat acctattttt
3180taggctttgt tatgattaca tcaaatgtgg acttcataca tagaaatcaa
cgcttacagg 3240tgtccttttt taagaatttc atacataaga tcatgatgaa
caatgggact acaaaatgaa 3300ataaagaaaa aatagaaata gaatagaaga
tcaattatta atcgccctat tcttccttat 3360tacctacaca aaataaagca
gcaacataag aaacaaaaac aaaatgaaaa caaaccaaat 3420aaatctatgt
aagcatactc atttcaattt gatattcatt acttgacttt tttgtcctta
3480tttgaggctc cataagcgcg ccattttccc ctactccctt ttttcgtaaa
tagtaataat 3540gtgctgaaaa gaacaatgaa gtagttatca tacatattcc
gtcgtgtcga tatgagggga 3600ggtgtctctt tctttcatcc cttgtcgcaa
cctccaatat ataagagcat aagcaactga 3660tcttacttta gtaattaact
tagcatacct ggcccgaagg aagaaaaaaa attcacctca 3720acaacatggt
tcctaagttt tacaaacttt caaacggctt caaaatccca agcattgctt
3780tgggaaccta ccggtgttta aaccccagcg cctggcgggg atattccaag
atcgcaaaca 3840gccgaaattg tgtatgaagg tgtcaagtgc ggctaccgtc
atttcgatac tgctgttctt 3900tatggtaatg agaaggaagt tggcgatggt
atcattaaat ggttgaacga agatccaggg 3960aaccataaac gtgaggaaat
cttctacact actaaattat ggaattcgca aaacggatat 4020aaaagagcta
aagctgccat tcagcaatgt ttgaatgaag tctcgggctt gcaatacatc
4080gatcttcttt tgattcattc gccactggaa ggttctaaat taaggttgga
aacttggcgc 4140gccatgcaag aagcggttga tgaaggattg gttaagtcta
taggggtttc caactatggg 4200aaaaagcaca ttgatgaact tttgaactgg
ccagaactga agcacaagcc agtggtcaac 4260caaatcgaga tatcaccttg
gattatgaga caagaattag 4300583586DNAArtificial SequenceSynthetic
GFP_SFC1 donor cassette 58ccggtggtgc ttatactgtt tctactgcag
ctgccgctac tgttagatct accatcagaa 60gattaagaga aatggttgaa gcttaaactt
ctttcattca ttttctcttg gctttcacta 120ggtatatcta ttccataacg
actatgtttt gtatttgtta atttacataa aaccatatca 180gtacatcaac
gaactgtaaa aaagaaactt tagcataatt attgcggata tttaaactca
240cttgcaggta agagcaaaag gcgattgatt tccagtccgc ctcttgtcac
gtgatttagt 300aagaaatttt gacagcaccc tcggtttaat ggaaaagagg
ggcgtttttc gatgaacccg 360aggggagact agagaatcat ctcggttgaa
tggagcatta tttttttagt agcgcccgcc 420cggagaaatg gacgttggcg
aatgagccat gaattattaa ccgcccatgt ctaccagata 480gacggcacgg
ccacgcgttt aaaccgcccc acatcgtatg acagtaccag cctagtcccg
540gtaaaccgca aacggacctt aattgtgacg aagggcccaa atttgatggg
tcggtgttaa 600tgattagtcc tcattgtcat aataaagtgt gatgatggag
gcaatgatga tatacggtag 660tactactgct cgaggtgcta tcttttaacc
aatcctttga gattcttgtc gccacggagt 720tactaccttt tacaaaccgt
aatgtcacat tttgcatata tcttatgtat aaatatatag 780ttcacttact
acttgttctc gttttgttaa ctttcttgtt gtagttcttc ttgttcttgg
840cgtttccccc tttgttttct atctgcttca taagtaaagt gcaaagcatt
ttggaagata 900ttatcaattg agtcattgaa agaaacttgg catcttccct
attactaaaa ctaagaatac 960ttgattcaag aaagaagttt atattagttt
tagccgtaag ataacataac aaagaagaag 1020aaagaaaaac acaattacag
taacaataac aagaggacag atactaccaa aatgtgtggg 1080gaagcgggta
agctgccaca gcaattaatg cacaacattt aacctacatt cttccttatc
1140ggatcctcaa aacccttaaa aacatatgcc tcaccctaac atattttcca
attaaccctc 1200aatatttctc tgtcacccgg cctctatttt ccattttctt
ctttacccgc cacgcgtttt 1260tttctttcaa atttttttct tctttcttct
ttttcttcca cgtcctcttg cataaataaa 1320taaaccgttt tgaaaccaaa
ctcgcctctc tctctccttt ttgaaatatt tttgggtttg 1380tttgatcctt
tccttcccaa tctctcttgt ttaatatata ttcatttata tcacgctctc
1440tttttatctt cctttttttc ctctctcttg tattcttcct tcccctttct
actcaaacca 1500agaagaaaaa gaaaaggtca atctttgtta aagaatagga
tcttctacta catcagcttt 1560tagatttttc acgcttactg cttttttctt
cccaagatcg aaaatttact gaattaacaa 1620tggtgagcaa gggcgaggag
ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1680gcgacgtaaa
cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
1740gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
tggcccaccc 1800tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
ctaccccgac cacatgaagc 1860agcacgactt cttcaagtcc gccatgcccg
aaggctacgt ccaggagcgc accatcttct 1920tcaaggacga cggcaactac
aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 1980tgaaccgcat
cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
2040agctggagta caactacaac agccacaagg tctatatcac cgccgacaag
cagaagaacg 2100gcatcaaggt gaacttcaag acccgccaca acatcgagga
cggcagcgtg cagctcgccg 2160accactacca gcagaacacc cccatcggcg
acggccccgt gctgctgccc gacaaccact 2220acctgagcac ccagtccgcc
ctgagcaaag accccaacga gaagcgcgat cacatggtcc 2280tgctggagtt
cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac
2340tcgagaagct tgatccggct gcgaatttct tatgatttat gatttttatt
attaaataag 2400ttataaaaaa aataagtgta tacaaatttt aaagtgactc
ttaggtttta aaacgaaaat 2460tcttattctt gagtaactct ttcctgtagg
tcaggttgct ttctcaggta tagcatgagg 2520tcgctcttat tgaccacacc
tctaccggca tgccgagcat gatgaacaat gggactacaa 2580aatgaaataa
agaaaaaata gaaatagaat agaagatcaa ttattaatcg ccctattctt
2640ccttattacc tacacaaaat aaagcagcaa cataagaaac aaaaacaaaa
tgaaaacaaa 2700ccaaataaat ctatgtaagc atactcattt caatttgata
ttcattactt gacttttttg 2760tccttatttg aggctccata agcgcgccat
tttcccctac tccctttttt cgtaaatagt 2820aataatgtgc tgaaaagaac
aatgaagtag ttatcataca tattccgtcg tgtcgatatg 2880aggggaggtg
tctctttctt tcatcccttg tcgcaacctc caatatataa gagcataagc
2940aactgatctt actttagtaa ttaacttagc atacctggcc cgaaggaaga
aaaaaaattc 3000acctcaacaa catggttcct aagttttaca aactttcaaa
cggcttcaaa atcccaagca 3060ttgctttggg aacctaccgg tgtttaaacc
ccagcgcctg gcggggatat tccaagatcg 3120caaacagccg aaattgtgta
tgaaggtgtc aagtgcggct accgtcattt cgatactgct 3180gttctttatg
gtaatgagaa ggaagttggc gatggtatca ttaaatggtt gaacgaagat
3240ccagggaacc ataaacgtga ggaaatcttc tacactacta aattatggaa
ttcgcaaaac 3300ggatataaaa gagctaaagc tgccattcag caatgtttga
atgaagtctc gggcttgcaa 3360tacatcgatc ttcttttgat tcattcgcca
ctggaaggtt ctaaattaag gttggaaact 3420tggcgcgcca tgcaagaagc
ggttgatgaa ggattggtta agtctatagg ggtttccaac 3480tatgggaaaa
agcacattga tgaacttttg aactggccag aactgaagca caagccagtg
3540gtcaaccaaa tcgagatatc accttggatt atgagacaag aattag
3586594300DNAArtificial SequenceSynthetic ADE2_YJR030C donor
cassette 59ggaactttat gaacatatta agtctgcttg atgaaatgtc atgtgcaggt
gccgttggca 60caaaatggga acaaaattat gaaaattcag tcgaggatgg gtgtgaagca
cctgaatcca 120atccataccg ttctattatt gatctttcta gtcgttccat
taatataact gcagacctac 180tttcgactgt tgggaggagt aattcagcac
tcaacaagaa tgagattata gctgctattc 240aaggtctcgc ccatcaatgc
ctcaatccct gtgacgaact aggtatgcaa gcattgcaag 300cattagagaa
cattctgtta tctcgagcaa gtcaactacg tacggaaaaa gttgcggtgg
360ataacctact agagacagga ttattaccga tttttgagtt ggatgaaatc
caagatgtca 420agatgaaacg aattactagc attttatccg ttctttctaa
aatgacggca cggccacgcg
480tttaaaccgc ccttcttggg tcaacttgtg gaaggcgtaa caagtaacga
aacttttttg 540agagtgctta acgtatttaa caagtatgta gatgatccta
cggtggaaag gcagttgcaa 600gagttgatta tttctaagag ggaaatcgag
aaggagtaga ccaacgataa tgtaactata 660ccaagaaact tagtatgatg
gaattttttc aaggagtcgt aaattagatt ttcgcaggta 720ataatgcgat
atataagcaa tctcatttaa atatcgacgg tggcatttat accatcattt
780actgttatat ttatctaacg cgtcgcgacg cgttagataa aatacaacaa
gatttttttt 840tcgtgtcacc aatggcatga aagcttcgag aataatttga
aggaaatttc acttaatggg 900aaaaataaaa atgtaccctt atcgagatta
ctcttttacc ctcagttcaa ttaaaattca 960tcatgaacca agtaaaagtt
cctctaatta cgaacgagca agcaaattag tattgtgtgg 1020gagacgggtt
cttgaataat acataacttt tcttaaaaga atcaaagaca gataaaattt
1080aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat
aacactgaca 1140tctttaacaa cttttaatta tgatacattt cttacgtcat
gattgattat tacagctatg 1200ctgacaaatg actcttgttg catggctacg
aaccgggtaa tactaagtga ttgactcttg 1260ctgacctttt attaagaact
aaatggacaa tattatggag catttcatgt ataaattggt 1320gcgtaaaatc
gttggatctc tcttctaagt acatcctact ataacaatca agaaaaacaa
1380gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt
aggaggggga 1440caattgggac gtatgattgt tgaggcagca aacaggctca
acattaagac ggtaatacta 1500gatgctgaaa attctcctgc caaacaaata
agcaactcca atgaccacgt taatggctcc 1560ttttccaatc ctcttgatat
cgaaaaacta gctgaaaaat gtgatgtgct aacgattgag 1620attgagcatg
ttgatgttcc tacactaaag aatcttcaag taaaacatcc caaattaaaa
1680atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca
aaaagagcat 1740ttaatcaaaa atggtatagc agttacccaa agtgttcctg
tggaacaagc cagtgagacg 1800tccctattga atgttggaag agatttgggt
tttccattcg tcttgaagtc gaggactttg 1860gcatacgatg gaagaggtaa
cttcgttgta aagaataagg aaatgattcc ggaagctttg 1920gaagtactga
aggatcgtcc tttgtacgcc gaaaaatggg caccatttac taaagaatta
1980gcagtcatga ttgtgaggtc tgttaacggt ttagtgtttt cttacccaat
tgtagagact 2040atccacaagg acaatatttg tgacttatgt tatgcgcctg
ctagagttcc ggactccgtt 2100caacttaagg cgaagttgtt ggcagaaaat
gcaatcaaat cttttcccgg ttgtggtata 2160tttggtgtgg aaatgttcta
tttagaaaca ggggaattgc ttattaacga aattgcccca 2220aggcctcaca
actctggaca ttataccatt gatgcttgcg tcacttctca atttgaagct
2280catttgagat caatattgga tttgccaatg ccaaagaatt tcacatcttt
ctccaccatt 2340acaacgaacg ccattatgct aaatgttctt ggagacaaac
atacaaaaga taaagagcta 2400gaaacttgcg aaagagcatt ggcgactcca
ggttcctcag tgtacttata tggaaaagag 2460tctagaccta acagaaaagt
aggtcacata aatattattg cctccagtat ggcggaatgt 2520gaacaaaggc
tgaactacat tacaggtaga actgatattc caatcaaaat ctctgtcgct
2580caaaagttgg acttggaagc aatggtcaaa ccattggttg gaatcatcat
gggatcagac 2640tctgacttgc cggtaatgtc tgccgcatgt gcggttttaa
aagattttgg cgttccattt 2700gaagtgacaa tagtctctgc tcatagaact
ccacatagga tgtcagcata tgctatttcc 2760gcaagcaagc gtggaattaa
aacaattatc gctggagctg gtggggctgc tcacttgcca 2820ggtatggtgg
ctgcaatgac accacttcct gtcatcggtg tgcccgtaaa aggttcttgt
2880ctagatggag tagattcttt acattcaatt gtgcaaatgc ctagaggtgt
tccagtagct 2940accgtcgcta ttaataatag tacgaacgct gcgctgttgg
ctgtcagact gcttggcgct 3000tatgattcaa gttatacaac gaaaatggaa
cagtttttat taaagcaaga agaagaagtt 3060cttgtcaaag cacaaaagtt
agaaactgtc ggttacgaag cttatctaga aaacaagtaa 3120tatataagtt
tattgatata cttgtacagc aaataattat aaaatgatat acctattttt
3180taggctttgt tatgattaca tcaaatgtgg acttcataca tagaaatcaa
cgcttacagg 3240tgtccttttt taagaatttc atacataaga tcatgttgag
ataattgttg ggattccatt 3300gttgataaag gctataatat taggtataca
gaatatacta gaagttctcc tcgaggatat 3360aggaatcctc aaaatggaat
ctatatttct acatactaat attacgatta ttcctcattc 3420cgttttatat
gtttatattc attgatccta ttacattatc aatccttgcg tttcagcttc
3480ctctaacttc gatgacagct tctcataact tatgtcatca tcttaacacc
gtatatgata 3540atatattgat aatataacta ttagttgata gacgatagtg
gatttttatt ccaacatacc 3600acccataatg taatagatct aatgaatcca
tttgtttgtt aatagtttga atgtttttat 3660cggaagaggt ttggtcatta
cgtctgcaat attctttttg gtttcgatat agcatacgtg 3720cagatgattt
cctgatactt catctctcaa tctcattgct ttagtaccaa aaaatctgtt
3780cctaaatttc cggtgtttaa accccagcgc ctggcgggtc ttcattattg
gatataatta 3840tactgattgt agatttactg tcggttagta atcctttggt
aattggtttc ttgtcaagtt 3900cttgtatcag gtaacttaga ttatttaata
atgggacaga ttcacttatc gcgtgtattt 3960ctgcttccgt agttgaagta
catgttaatg aagccttggt ggactttcct ccaattacct 4020ttccattaag
taaatatatg ttgccaattt gtgatttata atacggttgg ttgccatacg
4080aggcatcgct tataacaact aatttatttg ttggcttaac aggtttgctt
ttgtgccata 4140ttaattgctt atctctcgta ttccatatga actgtatcaa
ttcatatgtc atatctaaca 4200cttgcttgga cggaaatagt atatgttgtg
caagtgtgtt gatgtagtat aataggtcaa 4260atctaaattt atatccaaca
tatgatgcta gacctatcag 4300603586DNAArtificial SequenceSynthetic
GFP_YJR030C donor cassette 60ggaactttat gaacatatta agtctgcttg
atgaaatgtc atgtgcaggt gccgttggca 60caaaatggga acaaaattat gaaaattcag
tcgaggatgg gtgtgaagca cctgaatcca 120atccataccg ttctattatt
gatctttcta gtcgttccat taatataact gcagacctac 180tttcgactgt
tgggaggagt aattcagcac tcaacaagaa tgagattata gctgctattc
240aaggtctcgc ccatcaatgc ctcaatccct gtgacgaact aggtatgcaa
gcattgcaag 300cattagagaa cattctgtta tctcgagcaa gtcaactacg
tacggaaaaa gttgcggtgg 360ataacctact agagacagga ttattaccga
tttttgagtt ggatgaaatc caagatgtca 420agatgaaacg aattactagc
attttatccg ttctttctaa aatgacggca cggccacgcg 480tttaaaccgc
ccttcttggg tcaacttgtg gaaggcgtaa caagtaacga aacttttttg
540agagtgctta acgtatttaa caagtatgta gatgatccta cggtggaaag
gcagttgcaa 600gagttgatta tttctaagag ggaaatcgag aaggagtaga
ccaacgataa tgtaactata 660ccaagaaact tagtatgatg gaattttttc
aaggagtcgt aaattagatt ttcgcaggta 720ataatgcgat atataagcaa
tctcatttaa atatcgacgg tggcatttat accatcattt 780actgttatat
ttatctaacg cgtcgcgacg cgttagataa aatacaacaa gatttttttt
840tcgtgtcacc aatggcatga aagcttcgag aataatttga aggaaatttc
acttaatggg 900aaaaataaaa atgtaccctt atcgagatta ctcttttacc
ctcagttcaa ttaaaattca 960tcatgaacca agtaaaagtt cctctaatta
cgaacgagca agcaaattag tattgtgtgg 1020gagacgggac acaattacag
taacaataac aagaggacag atactaccaa aatgtgtggg 1080gaagcgggta
agctgccaca gcaattaatg cacaacattt aacctacatt cttccttatc
1140ggatcctcaa aacccttaaa aacatatgcc tcaccctaac atattttcca
attaaccctc 1200aatatttctc tgtcacccgg cctctatttt ccattttctt
ctttacccgc cacgcgtttt 1260tttctttcaa atttttttct tctttcttct
ttttcttcca cgtcctcttg cataaataaa 1320taaaccgttt tgaaaccaaa
ctcgcctctc tctctccttt ttgaaatatt tttgggtttg 1380tttgatcctt
tccttcccaa tctctcttgt ttaatatata ttcatttata tcacgctctc
1440tttttatctt cctttttttc ctctctcttg tattcttcct tcccctttct
actcaaacca 1500agaagaaaaa gaaaaggtca atctttgtta aagaatagga
tcttctacta catcagcttt 1560tagatttttc acgcttactg cttttttctt
cccaagatcg aaaatttact gaattaacaa 1620tggtgagcaa gggcgaggag
ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1680gcgacgtaaa
cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
1740gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
tggcccaccc 1800tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
ctaccccgac cacatgaagc 1860agcacgactt cttcaagtcc gccatgcccg
aaggctacgt ccaggagcgc accatcttct 1920tcaaggacga cggcaactac
aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 1980tgaaccgcat
cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
2040agctggagta caactacaac agccacaagg tctatatcac cgccgacaag
cagaagaacg 2100gcatcaaggt gaacttcaag acccgccaca acatcgagga
cggcagcgtg cagctcgccg 2160accactacca gcagaacacc cccatcggcg
acggccccgt gctgctgccc gacaaccact 2220acctgagcac ccagtccgcc
ctgagcaaag accccaacga gaagcgcgat cacatggtcc 2280tgctggagtt
cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac
2340tcgagaagct tgatccggct gcgaatttct tatgatttat gatttttatt
attaaataag 2400ttataaaaaa aataagtgta tacaaatttt aaagtgactc
ttaggtttta aaacgaaaat 2460tcttattctt gagtaactct ttcctgtagg
tcaggttgct ttctcaggta tagcatgagg 2520tcgctcttat tgaccacacc
tctaccggca tgccgagcat gttgagataa ttgttgggat 2580tccattgttg
ataaaggcta taatattagg tatacagaat atactagaag ttctcctcga
2640ggatatagga atcctcaaaa tggaatctat atttctacat actaatatta
cgattattcc 2700tcattccgtt ttatatgttt atattcattg atcctattac
attatcaatc cttgcgtttc 2760agcttcctct aacttcgatg acagcttctc
ataacttatg tcatcatctt aacaccgtat 2820atgataatat attgataata
taactattag ttgatagacg atagtggatt tttattccaa 2880cataccaccc
ataatgtaat agatctaatg aatccatttg tttgttaata gtttgaatgt
2940ttttatcgga agaggtttgg tcattacgtc tgcaatattc tttttggttt
cgatatagca 3000tacgtgcaga tgatttcctg atacttcatc tctcaatctc
attgctttag taccaaaaaa 3060tctgttccta aatttccggt gtttaaaccc
cagcgcctgg cgggtcttca ttattggata 3120taattatact gattgtagat
ttactgtcgg ttagtaatcc tttggtaatt ggtttcttgt 3180caagttcttg
tatcaggtaa cttagattat ttaataatgg gacagattca cttatcgcgt
3240gtatttctgc ttccgtagtt gaagtacatg ttaatgaagc cttggtggac
tttcctccaa 3300ttacctttcc attaagtaaa tatatgttgc caatttgtga
tttataatac ggttggttgc 3360catacgaggc atcgcttata acaactaatt
tatttgttgg cttaacaggt ttgcttttgt 3420gccatattaa ttgcttatct
ctcgtattcc atatgaactg tatcaattca tatgtcatat 3480ctaacacttg
cttggacgga aatagtatat gttgtgcaag tgtgttgatg tagtataata
3540ggtcaaatct aaatttatat ccaacatatg atgctagacc tatcag
35866143DNAArtificial SequenceSynthetic Recognition sequence for
ZFN.YJR030C 61cccggtatca gcaacccccc atgacgataa cgttgatgaa acg
436240DNAArtificial SequenceSynthetic Recognition sequence for
ZFN.SFC1 62cacctttacc gtttatgaat atgtaaggga gcatttagaa
406327DNAArtificial SequenceSynthetic Primer CUT351 63gcgaatgagc
catgaattat taaccgc 276433DNAArtificial SequenceSynthetic Primer
CUT350 64agatgaaacg aattactagc attttatccg ttc 336547DNAArtificial
SequenceSynthetic Primer CUT371 65taactaccat tactcagtgt acttgattgt
tttgtccgat tttcttg 476622DNAArtificial SequenceSynthetic Primer
HJ788 66gccgggtgac agagaaatat tg 22
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