U.S. patent application number 15/514617 was filed with the patent office on 2017-08-03 for composition for hair loss prevention or hair growth stimulation comprising artemisia umbelliformis extract.
This patent application is currently assigned to AMOREPACIFIC CORPORATION. The applicant listed for this patent is AMOREPACIFIC CORPORATION. Invention is credited to Eun Gyung CHO, Hae Kwang LEE, Jon Hwan LEE, Tae Ryong LEE, Pil Joon PARK, Seung Hyun SHIN.
Application Number | 20170216386 15/514617 |
Document ID | / |
Family ID | 55653345 |
Filed Date | 2017-08-03 |
United States Patent
Application |
20170216386 |
Kind Code |
A1 |
PARK; Pil Joon ; et
al. |
August 3, 2017 |
COMPOSITION FOR HAIR LOSS PREVENTION OR HAIR GROWTH STIMULATION
COMPRISING ARTEMISIA UMBELLIFORMIS EXTRACT
Abstract
Disclosed is a novel use of a composition comprising as an
active ingredient an Artemisia umbelliformis extract. A composition
according to the present description shows hair follicle cell
proliferation or hair growth effect by means of comprising an
Artemisia umbelliformis extract. Therefore, due to said effect, the
Artemisia umbelliformis extract has hair loss prevention or hair
growth stimulation effect.
Inventors: |
PARK; Pil Joon; (Yongin-si,
Gyeonggi-do, KR) ; SHIN; Seung Hyun; (Yongin-si,
Gyeonggi-do, KR) ; LEE; Hae Kwang; (Yongin-si,
Gyeonggi-do, KR) ; LEE; Jon Hwan; (Yongin-si,
Gyeonggi-do, KR) ; LEE; Tae Ryong; (Yongin-si,
Gyeonggi-do, KR) ; CHO; Eun Gyung; (Yongin-si,
Gyeonggi-do, KR) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
AMOREPACIFIC CORPORATION |
Seoul |
|
KR |
|
|
Assignee: |
AMOREPACIFIC CORPORATION
Seoul
KR
|
Family ID: |
55653345 |
Appl. No.: |
15/514617 |
Filed: |
September 24, 2015 |
PCT Filed: |
September 24, 2015 |
PCT NO: |
PCT/KR2015/010135 |
371 Date: |
March 27, 2017 |
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A61K 8/9789 20170801;
A61K 47/10 20130101; A61K 9/08 20130101; A61K 9/2018 20130101; A61Q
7/00 20130101; A61K 9/1652 20130101; A61K 36/282 20130101; A61P
17/14 20180101; A61K 9/0014 20130101; A61K 9/0019 20130101; A61K
9/4866 20130101; A61P 17/12 20180101; A61K 36/539 20130101; A61K
9/4858 20130101; A61P 43/00 20180101; A61K 9/1623 20130101; A61K
9/205 20130101 |
International
Class: |
A61K 36/539 20060101
A61K036/539; A61K 9/00 20060101 A61K009/00; A61K 47/10 20060101
A61K047/10 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 6, 2014 |
KR |
10-2014-0134591 |
Claims
1. A method for preventing hair loss or accelerating hair growth,
comprising administering an effective amount of an Alpine skullcap
(Scutellaria alpina) extract to a subject in need thereof.
2. The method according to claim 1, wherein the prevention of hair
loss or acceleration of hair growth is achieved by promoting
proliferation of hair follicle cells or growth of hair.
3. The method according to claim 2, wherein the hair follicle cell
is a hair dermal papilla cell or a hair germinal matrix cell.
4. The method according to claim 1, wherein the Alpine skullcap is
one or more selected from a group consisting of a leaf, a flower, a
stem and a root of the Alpine skullcap.
5. The method according to claim 1, wherein the extract is
comprised in a composition, wherein a concentration of the Alpine
skullcap extract in the composition is 1-70 ppm (w/w) based on the
total weight of the composition.
6. The method according to claim 5, wherein the concentration of
the Alpine skullcap extract in the composition is 1-50 ppm (w/w)
based on the total weight of the composition.
7. The method according to claim 1, wherein the Alpine skullcap
extract is an extract of one or more selected from a group
consisting of a water, an organic solvent and a mixture
thereof.
8. The method according to claim 7, wherein the organic solvent is
one or more selected from a group consisting of a C.sub.1-C.sub.6
lower alcohol, butylene glycol and propylene glycol.
9. The method according to claim 8, wherein the lower alcohol is
ethanol.
10. The method according to claim 1, wherein the extract is
comprised in a composition, wherein the composition is a
pharmaceutical, food or cosmetic composition.
11. The method according to claim 1, wherein the extract is
comprised in a composition wherein the composition is a formulation
for oral administration, transdermal administration, subcutaneous
injection or external application to skin.
Description
TECHNICAL FIELD
[0001] The present disclosure relates to a composition containing
an Alpine wormwood (Artemisia umbelliformis) extract.
BACKGROUND ART
[0002] Whereas most animals shed and regrow hair seasonally, humans
shed about 100 strands of hair out of about 100,000 strands and
about 100 strands regrow every day, thus always maintaining a
similar number.
[0003] The hair growth cycle consists of anagen, catagen and
telogen phases. During the anagen phase, hair growth is promoted as
cell division occurs actively in the hair dermal papilla, and hair
grows only in this stage. Considering that the anagen is about 3-5
years for men and about 4-6 years for women, about 80-85% of hair
is in the anagen phase. In the catagen phase, which lasts about 3-4
weeks, the cell division declines gradually. Lastly, in the telogen
phase, the hair dermal papilla is withdrawn and the hair separated
from the capillary vessel and simply stuck in the scalp. This lasts
about 3 months and the hair in the telogen phase is easily lost
upon physical stimulation.
[0004] Although hair is not an organ critical in sustaining life,
it is an indicator of health state and an important part of the
body in terms of appearance. Whereas hair loss is considered a
normal physiological event for those with a lot of hair, those who
suffer from severe hair loss can be badly affected in terms of
mental well-being and quality of life due to depression, sense of
shame, social isolation, etc.
[0005] Although promotion of hair growth and prevention of hair
loss have been studied from ancient times, the mechanisms of hair
growth and hair loss have not been elucidated clearly yet.
Recently, cytological, biochemical or molecular biological studies
are actively carried out globally on hair growth and hair loss in
many research institutes of universities and companies. Also, a lot
of efforts are being made on the development of a drug that can
treat hair loss and promote hair growth.
[0006] (Patent document 1) KR10-2010-0116882 A.
DISCLOSURE
Technical Problem
[0007] In an aspect, the present disclosure is directed to a use
for preventing hair loss or accelerating hair growth.
[0008] In another aspect, the present disclosure is directed to
preventing hair loss or accelerating hair growth by promoting
proliferation of hair follicle cells or growth of hair.
Technical Solution
[0009] In an aspect, the present disclosure provides a composition
for preventing hair loss or accelerating hair growth, containing an
extract of Alpine wormwood (Artemisia umbelliformis), which belongs
to the genus Artemisia, as an active ingredient.
Advantageous Effects
[0010] In an aspect, because the composition containing an Alpine
wormwood (Artemisia umbelliformis) extract as an active ingredient
of the present disclosure promotes proliferation of hair follicle
cells or growth of hair, it can provide an effect of preventing
hair loss or accelerating hair growth.
BRIEF DESCRIPTION OF DRAWINGS
[0011] FIG. 1 shows a result of an MTT assay on hair dermal papilla
cells of an Alpine wormwood (Artemisia umbelliformis) extract
according to an aspect of the present disclosure (hDPc
proliferation: viability of hair dermal papilla cells, AT: Alpine
wormwood extract).
[0012] FIG. 2 shows the effect of Alpine wormwood (Artemisia
umbelliformis) extract according to an aspect of the present
disclosure on hair growth (AT: Alpine wormwood extract).
BEST MODE
[0013] Korean Patent Application No. 10-2014-0134591, filed on Oct.
6, 2014, is incorporated herein in its entirety by reference. Also,
this application claims the benefits of Korean Patent Application
No. 10-2014-0134591 the contents of which in its entirety are
herein incorporated by reference.
[0014] Hereinafter, specific exemplary embodiments of the present
disclosure are described in detail such that those of ordinary
skill in the art to which the present disclosure belongs can easily
carry out the present disclosure.
[0015] In an aspect, the present disclosure may relate to a
composition containing an Alpine wormwood (Artemisia umbelliformis)
extract as an active ingredient. Specifically, in an aspect of the
present disclosure, the composition may be a composition for
preventing hair loss or accelerating hair growth.
[0016] In an aspect, the present disclosure may relate to a method
for preventing hair loss or accelerating hair growth, including
administering an Alpine wormwood (Artemisia umbelliformis) extract
to an individual in need of prevention of hair loss or acceleration
of hair growth.
[0017] In an aspect, the present disclosure may relate to a use of
an Alpine wormwood (Artemisia umbelliformis) extract for hair loss
prevention of hair loss or acceleration of hair growth.
[0018] In an aspect, the present disclosure may relate to an Alpine
wormwood (Artemisia umbelliformis) extract for use in prevention of
hair loss or acceleration of hair growth.
[0019] In an aspect of the present disclosure, the prevention of
hair loss or acceleration of hair growth may be achieved by
promoting proliferation of hair follicle cells or growth of
hair.
[0020] In the present disclosure, the term "hair loss" may refer to
absence of hair on the part where hair is normally present. For
example, it may mean depilation of hair from the scalp.
[0021] And, in the present disclosure, the term "acceleration of
hair growth" may refer to acceleration of not only growth of new
hair but also healthy growth of existing hair.
[0022] In an aspect of the present disclosure, the prevention of
hair loss or acceleration of hair growth may be achieved by
promoting proliferation of hair follicle cells or growth of hair.
Specifically, the proliferation of hair follicle cells may mean
proliferation of hair dermal papilla cells or hair germinal matrix
cells, although not being limited thereto.
[0023] In the present disclosure, the term "hair dermal papilla
cell" may refer to a cell separated from the dermal cell layer and
the hair dermal papilla cells may be covered with numerous hair
germinal matrix cells.
[0024] And, in the present disclosure, the term "hair germinal
matrix cell" may refer to a cell existing in the hair dermal
papilla cell tissue and capable of producing hair.
[0025] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) may be one or more selected from a group
consisting of the leaf, fruit, flower, stem and root of the plant
Alpine wormwood. Specifically, in an aspect of the present
disclosure, the Alpine wormwood (Artemisia umbelliformis) may be a
mixture of the flower, leaf and stem of the plant Alpine wormwood
(Artemisia umbelliformis).
[0026] In an aspect of the present disclosure, the concentration of
the Alpine wormwood (Artemisia umbelliformis) extract may be, based
on the total volume of the composition containing the same, 0.1-70
ppm (w/w), more specifically 0.1-50 ppm (w/w), although not being
limited thereto. Specifically, in an aspect of the present
disclosure, the concentration of the Alpine wormwood (Artemisia
umbelliformis) extract in the composition may be, based on the
total volume of the composition, 0.1 ppm or higher, 0.5 ppm or
higher, 0.6 ppm or higher, 0.7 ppm or higher, 0.8 ppm or higher,
0.9 ppm or higher, 1.0 ppm or higher, 1.3 ppm or higher, 1.5 ppm or
higher, 1.7 ppm or higher, 2.0 ppm or higher, 2.3 ppm or higher,
2.5 ppm or higher, 2.7 ppm or higher, 3.0 ppm or higher, 3.5 ppm or
higher, 4.0 ppm or higher, 4.1 ppm or higher, 4.2 ppm or higher,
4.3 ppm or higher, 4.4 ppm or higher, 4.5 ppm or higher, 4.6 ppm or
higher, 4.7 ppm or higher, 4.8 ppm or higher, 4.9 ppm or higher, 5
ppm or higher, 5.2 ppm or higher, 5.4 ppm or higher, 5.6 ppm or
higher, 5.8 ppm or higher, 6.0 ppm or higher, 6.5 ppm or higher,
7.0 ppm or higher, 7.5 ppm or higher, 8.0 ppm or higher, 9.0 ppm or
higher, 10.0 ppm or higher, 11.0 ppm or higher, 12.0 ppm or higher,
20 ppm or higher, 30 ppm or higher, 40 ppm or higher, 50 ppm or
higher or 60 ppm or higher, although not being limited thereto, and
may be 70 ppm or lower, 60 ppm or lower, 50 ppm or lower, 40 ppm or
lower, 30 ppm or lower, 20 ppm or lower, 15.0 ppm or lower, 10.0
ppm or lower, 8.0 ppm or lower, 6.0 ppm or lower, 5.8 ppm or lower,
5.6 ppm or lower, 5.4 ppm or lower, 5.2 ppm or lower, 5.0 ppm or
lower, 4.8 ppm or lower, 4.6 ppm or lower, 4.4 ppm or lower, 4.2
ppm or lower, 4.0 ppm or lower, 3.7 ppm or lower, 3.5 ppm or lower,
3.3 ppm or lower, 3.0 ppm or lower, 2.8 ppm or lower, 2.6 ppm or
lower, 2.4 ppm or lower, 2.2 ppm or lower, 2.0 ppm or lower, 1.8
ppm or lower, 1.6 ppm or lower, 1.4 ppm or lower, 1.2 ppm or lower,
1.0 ppm or lower , 0.8 ppm or lower , 0.6 ppm or lower or 0.3 ppm
or lower. The concentration of the extract is in ppm (w/w)
unit.
[0027] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) extract may be prepared by a method
including (1) a step of extracting Alpine wormwood (Artemisia
umbelliformis) with water, an organic solvent or a combination
thereof.
[0028] In an aspect of the present disclosure, the method may
further include, before the step (1), a step of processing the
Alpine wormwood (Artemisia umbelliformis). Specifically, the
processing may be drying and then pulverizing Alpine wormwood
(Artemisia umbelliformis) into powder. However, any processing for
making extraction easier is included, without being limited
thereto. The drying may be specifically sunlight drying, hot air
drying, evaporation drying, spray drying or freeze-drying, more
specifically hot air drying. Otherwise, in an aspect of the present
disclosure, live Alpine wormwood (Artemisia umbelliformis) may be
extracted per se without any processing.
[0029] In an aspect of the present disclosure, the method may
further include a step of removing the solvent through distillation
after the extraction. Specifically, the distillation may be vacuum
distillation.
[0030] In an aspect of the present disclosure, the method may
further include a step of adding one or more of glycerin and a
preservative to the concentrate after the distillation.
[0031] In an aspect of the present disclosure, the method may
further include a step of filtering after the step of removing the
solvent or the step of adding one or more of glycerin and a
preservative.
[0032] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) extract may be an extract of one or more
selected from a group consisting of water, an organic solvent and a
mixture thereof. Specifically, in an aspect of the present
disclosure, the organic solvent may be one or more selected from a
group consisting of a C.sub.1-C.sub.6 lower alcohol, butylene
glycol and propylene glycol. More specifically, the lower alcohol
may be ethanol.
[0033] In the present disclosure, the "Alpine wormwood (Artemisia
umbelliformis)" refers to a herb in the genus Artemisia of the
family Asteraceae, also known as White Genepi or Genepi Blanco. The
Alpine wormwood is used to produce the liquor called Genepi and the
leaves are used in the preparation of a tea or a condiment.
[0034] In an aspect of the present disclosure, the term "extract"
includes any substance that can be extracted from a natural
product, regardless of extraction method, extraction solvent,
extracted components or type of extract, and is used in a broad
concept, including any substance that can be obtained by processing
or treating otherwise the substance extracted from the natural
product. Specifically, the processing or treatment may be
fermenting or enzymatically treating the extract. Accordingly, in
the present disclosure, the extract includes a fermentation
product, a concentrate and a dried product. Specifically, in the
present disclosure, the extract may be a fermentation product.
[0035] In an aspect of the present disclosure, the "Alpine wormwood
(Artemisia umbelliformis) extract" includes any substance that can
be extracted from Alpine wormwood (Artemisia umbelliformis),
regardless of extraction method, extraction solvent, extracted
components or type of extract and any substance obtained during the
extraction process including treatment with heat, an acid, a base,
an enzyme, etc., and is used in a broad concept, including any
substance that can be obtained by processing or treating otherwise
the substance extracted from Alpine wormwood (Artemisia
umbelliformis). Accordingly, in an aspect of the present
disclosure, the Alpine wormwood (Artemisia umbelliformis) extract
may be a fermentation product.
[0036] In an aspect of the present disclosure, the "Alpine wormwood
(Artemisia umbelliformis)" may be in the form of an extract, live
Alpine wormwood (Artemisia umbelliformis), a pulverization product
of live Alpine wormwood (Artemisia umbelliformis), a dried product
of live Alpine wormwood (Artemisia umbelliformis), a dried
pulverization product live Alpine wormwood (Artemisia
umbelliformis) or a fermentation product of Alpine wormwood
(Artemisia umbelliformis), although not being limited thereto.
Also, the Alpine wormwood (Artemisia umbelliformis) used in the
present disclosure is not limited as to how it is acquired. For
example, it may be either cultivated or purchased commercially.
Also, all or part of the areal part or root part of the herbaceous
plant may be used. More specifically, one or more selected from a
group consisting of the leaf, stem, root and flower of the plant
Alpine wormwood (Artemisia umbelliformis) may be used. More
specifically, flower, leaf and stem may be used. In an aspect of
the present disclosure, the Alpine wormwood (Artemisia
umbelliformis) needs not necessarily be dried and the form of the
raw material is not limited as long as it is suitable to extract
the active ingredients of Alpine wormwood (Artemisia
umbelliformis).
[0037] In an aspect of the present disclosure, the water may
include distilled water or purified water, and the organic solvent
may include one or more selected from a group consisting of an
alcohol, e.g., a C.sub.1-C.sub.6 lower alcohol, acetone, ether,
ethyl acetate, diethyl ether, methyl ethyl ketone and chloroform,
although not being limited thereto.
[0038] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) extract may include a C.sub.1-C.sub.6
alcohol extract of Alpine wormwood (Artemisia umbelliformis).
Specifically, the alcohol may be methanol or ethanol.
[0039] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) extract may be obtained by a method
including a step of extracting Alpine wormwood (Artemisia
umbelliformis) with water, an organic solvent or a mixture
thereof.
[0040] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) extract may be a crude extract of a
solvent selected from a group consisting of water, an organic
solvent and a combination thereof. The organic solvent may be a
C.sub.1-C.sub.6 alcohol. Specifically, the C.sub.1-C.sub.6 alcohol
may be methanol or ethanol. In an aspect of the present disclosure,
the Alpine wormwood (Artemisia umbelliformis) may be extracted by
adding about 5-15 times, specifically about 10 times, of the
solvent to Alpine wormwood (Artemisia umbelliformis), although not
being limited thereto.
[0041] In an aspect of the present disclosure, the extraction may
be performed by hot water extraction, ethanol extraction, heating
extraction, cold precipitation extraction, reflux extraction,
reflux condensation extraction, ultrasonic extraction, etc. Any
extraction method obvious to those skilled in the art may be used
without limitation. Specifically, the extraction may be performed
by hot water extraction or ethanol extraction.
[0042] In an aspect of the present disclosure, the extraction may
be performed at elevated temperatures for more effective
extraction, although it may also be performed at room temperature.
The extraction may be performed specifically at about
40-100.degree. C., more specifically at about 80.degree. C.,
although not being limited thereto. The extraction may be performed
for about 2-14 hours, specifically for 8-14 hours, more
specifically for 11-13 hours, most specifically for 12 hours.
However, the extraction time may be varied depending on such
conditions as extraction solvent, extraction temperature, etc.
without being limited thereto. The extraction may be performed once
or several times in order to obtain the active ingredients in
larger quantities. The extraction may be performed specifically 1-5
times, more specifically 3 times continuously.
[0043] In an aspect of the present disclosure, the Alpine wormwood
(Artemisia umbelliformis) extract may include a crude extract of
Alpine wormwood (Artemisia umbelliformis) and may also include a
soluble fraction obtained by further extracting the crude extract
with an organic solvent of low polarity. In an aspect of the
present disclosure, the organic solvent may be hexane, methylene
chloride, ethyl acetate, n-butanol, etc., although not being
limited thereto. The obtained extract or the soluble fraction of
the extract may be used per se. Alternatively, it may be filtered
and then concentrated or may be dried after the concentration.
[0044] In an aspect of the present disclosure, the drying may be
evaporation drying, spray drying or freeze-drying. Specifically,
freeze-drying may be performed at -50 to -70.degree. C. for 3-4
days.
[0045] In an aspect of the present disclosure, the composition may
be a cosmetic, pharmaceutical or food composition.
[0046] Specifically, the cosmetic composition may be, for example,
a hair cosmetic, a body cosmetic, a foundation cosmetic, a makeup
cosmetic, etc., and the formulation is not specially limited and
may be selected adequately depending on purposes.
[0047] For example, the cosmetic composition may be formulated into
a solution, a suspension, an emulsion, a paste, a gel, a cream, a
lotion, a powder, a soap, a surfactant-containing cleanser, an oil,
a powder foundation, an emulsion foundation, a wax foundation, a
spray, etc., although not being limited thereto. More specifically,
it may be formulated into a cleanser such as a shampoo, a rinse and
a body cleanser, a hairstyling product such as a hair tonic, a gel,
a mousse, etc., a hair cosmetic composition such as a hair
nourisher, a hair dye, etc. or a foundation cosmetic such as a
softening lotion, a nourishing lotion, a lotion, a body lotion, a
nourishing cream, a massage cream, a moisturizing cream, a hand
cream, an essence, an eye cream, a cleansing cream, a cleansing
foam, a cleansing water, a pack, a gel, a patch, an oil-in-water
(O/W) emulsion, a water-in-oil (O/W) emulsion, etc.
[0048] The cosmetic composition may contain a cosmetically
acceptable medium or matrix and may be provided as any topically
acceptable formulation, e.g., a solution, gel, an anhydrous solid
or paste, an oil-in-water emulsion, a suspension, a microemulsion,
a microcapsule, a microgranule, an ionic (liposomal) and/or
nonionic vesicular dispersion, a cream, a skin lotion, a powder, an
ointment, a spray or a conceal stick. The composition may be
prepared according to a method commonly employed in the art.
[0049] When the formulation of the present disclosure is a solution
or an emulsion, a solvent, a solubilizer or an emulsifier may be
used as a carrier component. For example, water, ethanol,
isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl
benzoate, propylene glycol, 1,3-butylene glycol, glycerol aliphatic
ester, polyethylene glycol or a fatty acid ester of sorbitan may be
used.
[0050] When the formulation of the present disclosure is a
suspension, a liquid diluent such as water, ethanol or propylene
glycol, a suspending agent such as ethoxylated isostearyl alcohol,
polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester,
microcrystalline cellulose, aluminum metahydroxide, bentonite, agar
or tragacanth, etc. may be used as a carrier component.
[0051] When the formulation of the present disclosure is a paste, a
cream or a gel, animal oil, vegetable oil, wax, paraffin, starch,
tragacanth, a cellulose derivative, polyethylene glycol, silicone,
bentonite, silica, talc, zinc oxide, etc. may be used as a carrier
component.
[0052] When the formulation of the present disclosure is a powder
or a spray, lactose, talc, silica, aluminum hydroxide, calcium
silicate or polyamide powder may be used as a carrier component. In
particular, when the formulation is a spray, it may further contain
a propellant such as chlorofluorohydrocarbon, propane/butane or
dimethyl ether.
[0053] In an exemplary embodiment of the present disclosure, the
cosmetic composition may further contain a thickener. The thickener
contained in the cosmetic composition of the present disclosure may
be methyl cellulose, carboxymethyl cellulose, carboxymethyl
hydroxyguanine, hydroxymethyl cellulose, hydroxyethyl cellulose,
carboxyvinyl polymer, polyquaternium, cetearyl alcohol, stearic
acid, carrageenan, etc. Specifically, one or more of carboxymethyl
cellulose, carboxyvinyl polymer and polyquaternium may be used.
Most specifically, carboxyvinyl polymer may be used.
[0054] In an exemplary embodiment of the present disclosure, the
cosmetic composition may contain various adequate matrices and
additives as desired, and their kind and amount can be easily
determined by those skilled in the art. The composition may contain
acceptable additives if necessary. For example, additives commonly
used in the art such as a preservative, a pigment, etc. may be
contained additionally.
[0055] Specifically, the preservative may be phenoxyethanol,
1,2-hexanediol, etc. and a fragrance such as a synthetic fragrance
may be used.
[0056] In an exemplary embodiment of the present disclosure, the
cosmetic composition may contain a substance selected from a group
consisting of a water-soluble vitamin, an oil-soluble vitamin, a
polypeptide, a polysaccharide, a sphingolipid and a seaweed
extract. In addition, it may further contain an oil, a fat, a
humectant, an emollient, a surfactant, an organic or inorganic
pigment, an organic powder, a UV absorbent, a preservative, a
sterilizer, an antioxidant, a plant extract, a pH control agent, an
alcohol, a colorant, a fragrance, a blood circulation stimulant, a
cooling agent, an antiperspirant, purified water, etc.
[0057] However, the ingredients that may be contained in the
cosmetic composition are not limited thereto and, the amount of the
ingredients may be determined within a range not negatively
affecting the purpose and effect of the present disclosure.
[0058] In another aspect, the present disclosure relates to a
formulation for external application to skin, containing an Alpine
wormwood (Artemisia umbelliformis) extract as an active ingredient.
The formulation for external application to skin refers to any
formulation that can be applied to skin from outside and various
types of cosmetic formulations may be included therein.
[0059] In another aspect, the present disclosure relates to a
pharmaceutical composition for oral or parenteral administration.
Usually, the pharmaceutical composition is prepared into a
formulation using a commonly used diluent or excipient such as a
filler, an extender, a binder, a wetting agent, a disintegrant, a
surfactant, etc. Solid formulations for oral administration may
include a tablet, a pill, a powder, a granule, a soft or hard
capsule, etc. The solid formulation is prepared by mixing the
active ingredient with at least one excipient, e.g., starch,
calcium carbonate, sucrose, lactose, gelatin, etc. In addition to
the simple excipient, a lubricant such as magnesium stearate, talc,
etc. may also be used. Liquid formulations for oral administration
may include a suspension, a liquid medicine for internal use, an
emulsion, a syrup, etc. In addition to a commonly used simple
diluent such as water or liquid paraffin, various excipients such
as a humectant, a sweetener, an aromatic, a preservative, etc. may
also be contained. Formulations for parenteral administration may
include a sterilized aqueous solution, a nonaqueous solution, a
suspension, an emulsion, a freeze-dried product and a suppository.
The nonaqueous solution or suspension may contain propylene glycol,
polyethylene glycol, vegetable oil such as olive oil, an injectable
ester such as ethyl oleate, etc. As a base of the suppository,
witepsol, macrogol, tween 61, cocoa butter, laurin butter,
glycerogelatin, etc. may be used.
[0060] In an aspect of the present disclosure, the active
ingredient may be administered in the form of a pharmaceutically
acceptable salt and may be used either alone or in combination with
another pharmaceutically active compound. The salt is not
particularly limited as long as it is pharmaceutically acceptable.
For example, a hydrochloride, a sulfate, a nitrate, a phosphate, a
hydrofluoride, a hydrobromide, a formate, an acetate, a tartrate, a
lactate, a citrate, a fumarate, a maleate, a succinate, a
methanesulfonate, a benzenesulfonate, a toluenesulfonate, a
naphthalenesulfonate, etc. may be used.
[0061] In an aspect of the present disclosure, the composition may
be administered parenterally or orally depending on purposes and a
daily dosage may be 0.1-500 mg, specifically 1-100 mg, per kg body
weight. The pharmaceutical composition may be administered once or
several times a day. The administration dosage for a particular
patient may vary depending on the body weight, age, sex and diet of
the patient, administration time, administration method, excretion
rate, severity of disease, etc.
[0062] The pharmaceutical composition according to an aspect of the
present disclosure may be prepared into any formulation suitable
for a pharmaceutical composition, including an oral formulation
such as a powder, a granule, a tablet, a soft or hard capsule, a
suspension, an emulsion, a syrup, an aerosol, etc., a formulation
for external application to skin such as an ointment, a cream,
etc., a suppository, an injection, a sterile injectable solution,
etc., according to a commonly employed method. Specifically, it may
be prepared into an injection or a formulation for external
application to skin.
[0063] The composition according to an aspect of the present
disclosure may be administered to a mammal such as rat, mouse,
livestock, human, etc. through various routes including parenteral
and oral routes. Any mode of administration may be expected. For
example, it may be administered orally, transdermally,
intravenously, intramuscularly or subcutaneously.
[0064] The composition according to an aspect of the present
disclosure may be administered through various routes that can be
easily adopted by those skilled in the art. In particular, the
pharmaceutical composition according to an aspect of the present
disclosure may be administered as a formulation for external
application to skin by applying onto the skin surface.
[0065] In an aspect of the present disclosure, the composition may
be a food composition. The food composition may be a health
functional food composition.
[0066] The formulation of the food composition according to an
aspect of the present disclosure is not particularly limited. For
example, it may be formulated into a tablet, a granule, a powder, a
liquid formulation such as a drink, a caramel, a gel, a bar, etc.
Each formulation of the food composition may contain, in addition
to the active ingredient, various ingredients commonly used in the
related art. Those ingredients may be selected by those skilled in
the art without difficulty in consideration of the particular
formulation or purpose of use, and they may result in synergic
effect when used together.
[0067] Determination of the administration dose of the active
ingredient in food composition according to an aspect of the
present disclosure is within the level of those skilled in the art.
A daily dose may be, for example, 0.1-5000 mg/kg/day, more
specifically 50-500 mg/kg/day, although not being limited thereto.
The administration dose will vary depending on various factors,
including the age of the subject to be treated, physical condition,
presence of complication(s), or the like.
[0068] For example, the food composition according to an aspect of
the present disclosure may be prepared into various foods such as a
chewing gum, a caramel, a candy, a popsicle, etc., drinks such as a
soft drink, mineral water, an alcoholic beverage, etc. or health
functional foods including a vitamin and a mineral.
[0069] In an aspect of the present disclosure, the food composition
may further contain various nutrients, vitamins, minerals
(electrolytes), flavoring agents including synthetic and natural
flavoring agents, colorants, extenders (cheese, chocolate, etc.),
pectic acid and salts thereof, alginic acid and salts thereof,
organic acids, protective colloidal thickeners, pH control agents,
stabilizers, preservatives, glycerin, alcohols, carbonating agents
used in soft drinks, etc. In an aspect of the present disclosure,
the functional food composition may further contain a pulp for
preparing a natural fruit juice, a fruit drink or a vegetable
drink. These ingredients may be used either independently or in
combination. The mixing ratio of the additives is of no significant
importance. In an aspect of the present disclosure, the additives
may be contained in an amount of about 0-20 parts by weight based
on 100 parts by weight of the composition.
[0070] Hereinafter, the present disclosure will be described in
detail through an example and test examples. However, the following
example and test examples are for illustrative purposes only and it
will be apparent to those of ordinary skill in the art that the
scope of the present disclosure is not limited by the example and
test examples.
EXAMPLE 1
Preparation of Alpine wormwood (Artemisia umbelliformis)
Extract
[0071] The flower, leaf and stem parts of Alpine wormwood
(Artemisia umbelliformis) were harvested, hot air dried and then
pulverized into a powder. The powder was extracted with an
ethanol/water mixture and then ethanol was removed through vacuum
distillation. After adding glycerin and a preservative to the
concentrate, an Alpine wormwood (Artemisia umbelliformis) extract
was obtained finally through filtration. The Alpine wormwood
(Artemisia umbelliformis) extract (ALPAFLOR.RTM. ARTEMISIA AO)
prepared as described above was purchased from DSM (DSM Nutritional
Products Ltd, 4002 Basel, Switzerland) and used in the following
test examples.
TEST EXAMPLE 1
Isolation and Culturing of Hair Follicle Cells
[0072] Hair dermal papilla cells were isolated from the human
occipital scalp tissue using a microscope. The cells were cultured
on a 35-mm culture dish coated with type 1 collagen for 14 days. A
culture medium was replaced every 3 days. Specifically, the medium
was DMEM (Dulbecco's modified Eagle's medium; Gibco BRL,
Gaithersburg, Md.) containing 10 .mu.g/mL streptomycin (Gibco,
N.Y., USA), 100 u/mL penicillin (Gibco, N.Y., USA) and 20%
heat-inactivated fetal bovine serum (Lonza, Walkersville, Md.). The
cells were cultured in a CO.sub.2 incubator under a condition of 5%
CO.sub.2 and 37.degree. C.
[0073] When the hair dermal papilla cells covered 80% of the area
of the plate (80% confluence), the cells were collected using 0.25%
trypsin/10 mM EDTA (Gibco, N.Y., USA) and cultured further on a
plate (96-well plate; Nunc, Wiebaden, Germany) containing DMEM
supplemented with 10% FBS. The cells were placed on the 96-well
plate at a density of 2000 cells/well and were cultured for 24
hours in a CO.sub.2 incubator under a condition of 5% CO.sub.2 and
37.degree. C.
EXAMPLE 2
Measurement of Cell Proliferation (MTT Assay)
[0074] Each well of the 96-well plate was treated with an extract
of Alpine wormwood (Artemisia umbelliformis) (0, 1, 2, 5, 10, 20
ppm) for 96 hours.
[0075] After adding 70 .mu.g/mL of an MTT solution (Sigma, St
Louis, Mo.) to each well, the cells were cultured for 3 hours in a
CO.sub.2 incubator under a condition of 5% CO.sub.2 and 37.degree.
C. Then, after adding 100 .mu.L of DMSO (Sigma, St Louis, Mo.) to
each well, absorbance was measured at 570 nm. The result is shown
in FIG. 1.
[0076] As can be seen from FIG. 1, the hair dermal papilla cells
treated with the Alpine wormwood (Artemisia umbelliformis) extract
of Example 1 showed increased cell viability. The increase was
statistically significant. The statistical significance was tested
by the paired Student's t-test. p<0.05 was considered
statistically significant.
TEST EXAMPLE 2
Culturing of Human Hair Follicles
[0077] For experiment, 15 human hair follicle samples were taken
from each group (90 samples in total).
[0078] The hair follicle samples were cultured on a 24-well plate
(Nunc, Wiesbaden, Germany) containing 500 .mu.L of William's E
medium (Gibco, N.Y., USA) containing 2 mM L-glutamine (PAA, Coelbe,
Germany), 10 .mu.g/mL insulin, 10 ng/mL hydrocortisone (Sigma, St
Louis, Mo.), 0.1% fungizone (Gibco, N.Y., USA), 10 .mu.g/mL
streptomycin and 100 u/mL penicillin (Gibco, N.Y., USA), with 5-6
samples per well.
EXAMPLE 3
Observation of Growth of Hair Follicles
[0079] The 24-well plate was treated with an extract of Alpine
wormwood (Artemisia umbelliformis) (0, 10, 50 ppm). A sample not
treated with Alpine wormwood (Artemisia umbelliformis) was used as
a control group. The medium was replaced every 2-3 days.
[0080] After treating with the Alpine wormwood (Artemisia
umbelliformis) extract, the hair follicles were imaged using a
stereoscopic microscope (Dongwon CNS, Korea) and hair growth length
was measured using the ImageJ program. The result is shown in FIG.
2.
[0081] As can be seen from FIG. 2, the Alpine wormwood (Artemisia
umbelliformis) extract promoted hair length growth in hair
follicle, and the results are statistically significant.
[0082] Hereinafter, the formulation examples of the composition
according to an aspect of the present disclosure are described.
However, the following formulation examples are for illustrative
purposes only and do not limit the scope of the present
disclosure.
FORMULATION EXAMPLE 1
Soft Capsule
[0083] 8 mg of the Alpine wormwood (Artemisia umbelliformis)
extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 2 mg of
palm oil, 8 mg of hydrogenated vegetable oil, 4 mg of yellow
beeswax and 9 mg of lecithin were mixed and prepared into a soft
capsule-filling solution according to a commonly employed method. A
soft capsule was prepared by filling 400 mg of the solution per
capsule. Separately from this, a soft capsule sheet was prepared
from 66 parts by weight of gelatin, 24 parts by weight of glycerin
and 10 parts by weight of a sorbitol solution and filled with the
filling solution to prepare a soft capsule containing 400 mg of the
composition according to an aspect of the present disclosure.
FORMULATION EXAMPLE 2
Tablet
[0084] 8 mg of the Alpine wormwood (Artemisia umbelliformis)
extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg
of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose
were mixed and granulated using a fluidized-bed drier. After adding
6 mg of sugar ester, 500 mg of the resulting composition was
prepared into a tablet according to a commonly employed method.
FORMULATION EXAMPLE 3
Drink
[0085] 8 mg of the Alpine wormwood (Artemisia umbelliformis)
extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of
glucose, 0.6 g of citric acid and 25 g of oligosaccharide syrup
were mixed with 300 mL of purified water. 200 mL of the resulting
drink was filled in a bottle and sterilized at 130.degree. C. for
4-5 seconds.
FORMULATION EXAMPLE 4
Granule
[0086] 8 mg of the Alpine wormwood (Artemisia umbelliformis)
extract of Example 1, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg
of anhydrous crystalline glucose and 550 mg of starch were mixed
and granulated using a fluidized-bed drier. The prepared granule
was filled in a pouch.
FORMULATION EXAMPLE 5
Injection
[0087] An injection was prepared with the composition described in
Table 1 according to a commonly employed method.
TABLE-US-00001 TABLE 1 Ingredients Contents Alpine wormwood
(Artemisia umbelliformis) extract of 10-50 mg Example 1 Sterilized
distilled water for injection adequate pH control agent
adequate
FORMULATION EXAMPLE 6
Health Functional Food
[0088] A health functional food was prepared with the composition
described in Table 2 according to a commonly employed method.
TABLE-US-00002 TABLE 2 Ingredients Contents Alpine wormwood
(Artemisia umbelliformis) extract of 20 mg Example 1 Vitamin A
acetate 70 .mu.g Vitamin E 1.0 mg Vitamin B.sub.1 0.13 mg Vitamin
B.sub.2 0.15 mg Vitamin B.sub.6 0.5 mg Vitamin B.sub.12 0.2 .mu.g
Vitamin C 10 mg Biotin 10 .mu.g Nicotinamide 1.7 mg Folic acid 50
.mu.g Calcium pantothenate 0.5 mg Ferrous sulfate 1.75 mg Zinc
oxide 0.82 mg Magnesium carbonate 25.3 mg Potassium phosphate
monobasic 15 mg Calcium phosphate dibasic 55 mg Potassium citrate
90 mg Calcium carbonate 100 mg Magnesium chloride 24.8 mg
[0089] Although the above-described composition of the vitamin and
mineral mixtures is given as a specific example adequate for a
health functional food, it may be altered otherwise.
FORMULATION EXAMPLE 7
Health Drink
[0090] A health drink was prepared with the composition described
in Table 3 according to a commonly employed method.
TABLE-US-00003 TABLE 3 Ingredients Contents Alpine wormwood
(Artemisia umbelliformis) extract of 1000 mg Example 1 Citric acid
1000 mg Oligosaccharide 100 g Taurine 19 Purified water balance
[0091] The above ingredients were mixed and heated for about 1 hour
at 85.degree. C. while stirring according to a commonly employed
health drink preparation method. The resulting solution was
filtered and sterilized.
FORMULATION EXAMPLE 8
Shampoo
[0092] A shampoo was prepared with the composition described in
Table 4 according to a commonly employed method.
TABLE-US-00004 TABLE 4 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 0.2 Example 1 Ammonium laureth
sulfate 10.0 Glycol distearate 2.0 Cetyl alcohol 0.6 Cocamide MEA
0.5 Ammonium lauryl sulfate 6.0 Guar hydroxypropyltrimonium
chloride 0.15 Polyquaternium-10 0.1 Silicone 0.5
Distearyldimethylammonium chloride 0.2 Sodium cocoamphoacetate 4.0
Fragrance 1.0 Preservative 0.03 Citric acid adequate Purified water
balance
FORMULATION EXAMPLE 9
Rinse
[0093] A rinse was prepared with the composition described in Table
5 according to a commonly employed method.
TABLE-US-00005 TABLE 5 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 0.2 Example 1 Cetyl alcohol
3.0 Glycerin monostearate 1.0 Hydroxyethyl cellulose 1.0 Propylene
glycol 4.0 Methyl parahydroxybenzoate 0.2
Stearyldimethylbenzylammonium chloride, 25% 10.0 Fragrance adequate
Pigment adequate Citric acid adequate Purified water balance
FORMULATION EXAMPLE 10
Ointment
[0094] An ointment was prepared with the composition described in
Table 6 according to a commonly employed method.
TABLE-US-00006 TABLE 6 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 0.2 Example 1 Butylene glycol
4.0 Liquid paraffin 15.0 Beta-glucan 7.0 Carbomer 0.1
Caprylic/capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside
1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Beeswax 4.0 Glycerin
8.0 Preservative, pigment and fragrance adequate Purified water
balance
FORMULATION EXAMPLE 11
Massage Cream
[0095] A massage cream was prepared with the composition described
in Table 7 according to a commonly employed method.
TABLE-US-00007 TABLE 7 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 2.0 Example 1 Glycerin 8.0
Butylene glycol 4.0 Liquid paraffin 45.0 Beta-glucan 7.0 Carbomer
0.1 Caprylic/capric triglyceride 3.0 Beeswax 4.0 Cetearyl glucoside
1.5 Sorbitan sesquioleate 0.9 Vaseline 3.0 Paraffin 1.5
Preservative, pigment and fragrance adequate Purified water
balance
FORMULATION EXAMPLE 12
Hair Pack
[0096] A hair pack was prepared with the composition described in
Table 8 according to a commonly employed method.
TABLE-US-00008 TABLE 8 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 0.2 Example 1 Glycerin 4.0
Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta-glucan 7.0
Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol 6.0
Preservative, pigment and fragrance adequate Purified water
balance
FORMULATION EXAMPLE 12
Softening Lotion (Skin Lotion)
[0097] A softening lotion was prepared with the composition
described in Table 9 according to a commonly employed method.
TABLE-US-00009 TABLE 9 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 0.2 Example 1 Glycerin 3.0
Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1
PEG-12 nonyl phenyl ether 0.2 Polysorbate 80 0.4 Ethanol 10.0
Triethanolamine 0.1 Preservative, pigment and fragrance adequate
Purified water balance
FORMULATION EXAMPLE 13
Nourishing Lotion (Milk Lotion)
[0098] A nourishing lotion was prepared with the composition
described in Table 10 according to a commonly employed method.
TABLE-US-00010 TABLE 10 Ingredients Contents (wt %) Alpine wormwood
(Artemisia umbelliformis) extract of 1.0 Example 1 Glycerin 3.0
Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1
Beeswax 4.0 Polysorbate 60 1.5 Caprylic/capric triglyceride 5.0
Squalane 5.0 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Cetearyl
alcohol 1.0 Triethanolamine 0.2 Preservative, pigment and fragrance
adequate Purified water balance
[0099] While the exemplary embodiments have been shown and
described, it will be understood by those skilled in the art that
various changes in form and details may be made thereto without
departing from the spirit and scope of this disclosure as defined
by the appended claims.
* * * * *