Tuning Bacteriophage Host Range

Abstract

Various aspects and embodiments of the invention are directed to high-throughput phage-engineering methods and recombinant bacteriophages with tunable host ranges for controlling phage specificity.

Description

RELATED APPLICATION

[0001] This application is a continuation of U.S. application Ser. No. 14/478,657, filed Sep. 5, 2014, which claims the benefit under 35 U.S.C. .sctn.119(e) of U.S. provisional application No. 61/873,901, filed Sep. 5, 2013, each of which is incorporated by reference herein in its entirety.

BACKGROUND OF THE INVENTION

[0003] A bacteriophage ("phage") is a virus that can specifically infect host bacteria and reproduces at the expense of the host bacteria. Shortly after their discovery, phages were proposed as a means to control pathogenic bacteria (d'Herelle, F., Bulletin of the New York Academy of Medicine 7, 329 (1931)); however, a poor understanding of the relationship between bacteria and phages led to frequent treatment failures, and the emergence of readily-available chemical antibiotics made phage therapy obsolete (Carlton, R. M., Archivum immunologiae et therapiae experimentalis 47, 267 (1999)). Presently, with the rise of drug-resistant bacteria and the sharp decline in antibiotic discovery (Fischbach, M. A. et al. Science 325, 1089 (2009)), phage therapy is regaining attention.

[0004] The limited range of bacterial cell hosts for a single type of phage has been a major challenge to the development and approval of clinical phage-based products. Traditionally, a phage "cocktail" was used to address this challenge (Sulakvelidze, A., et al. Antimicrobial agents and chemotherapy 45, 649 (2001)). Still, the desire to broaden the host range by adding different types of phages to a phage cocktail must be balanced with another challenge of producing and testing well-defined multi-component combinations for government regulatory approval.

[0005] Further still, creating phage-based therapeutics and diagnostics is limited by the difficulty of engineering phages. Phage genomes are often too large to be handled efficiently in vitro and reside for short periods of time in bacteria, which makes it difficult to modify the genomes during the phage reproductive cycle. Thus, phage genome engineering is classically performed with allele replacement methods whereby a piece of the phage genome is cloned into an appropriate bacterial vector, remodeled using classical molecular biology, and the bacterium containing the resulting construct is infected with the phage. The phage then recombines with the plasmid to acquire the desired mutations. This process, though, is inefficient because many phages degrade resident DNA upon entry and because the lack of phage selectable markers often make screening for acquired characteristics labor intensive. Moreover, there are very large stretches of phage DNA that harbor toxic functions and thus prevent their manipulation within bacteria.

SUMMARY OF THE INVENTION

[0006] The present disclosure addresses the above challenges by providing, inter alia, recombinant bacteriophages with tunable host ranges for controlling phage host cell specificity and high-throughput bacteriophage engineering methods. Artificially controlling phage specificity contributes to practical applications such as, for example, bacteriophage therapy and bacterial identification by altering and/or expanding the range of host cell strains recognized and/or infected by particular types of bacteriophages. This is achieved, in some embodiments, by altering host recognition elements such as, for example, tail fibers of a particular type of bacteriophage. A bacteriophage, using its tail fibers, recognizes and adsorbs to the outer membrane of its host bacterial cell(s) (Weidel, W. Annu Rev Microbiol 12, 27-48 (1958)). Altering (e.g., swapping, mutating) the tail fibers of a bacteriophage can alter the range of host bacterial cells recognized by the bacteriophage. For example, a T3 bacteriophage may be modified to have tail fibers from one or more different types of bacteriophages (e.g., T7, SP6, yppR, K1-5, K11), thereby expanding the bacterial cell host range of the T3 bacteriophage to that of the one or more different types of bacteriophages. Thus, instead of using a cocktail of different types of bacteriophage to try to target multiple different strains of pathogenic bacteria, the present disclosure contemplates, in some embodiments, the use of a cocktail of one type of recombinant bacteriophage with heterologous host recognition elements (e.g., heterologous tail fibers). Accordingly, various aspects of the present disclosure provide compositions that comprise recombinant bacteriophages with heterologous host recognition elements.

[0007] Methods of the present disclosure for altering bacteriophage host range overcome some of the difficulties of phage engineering, particularly those associated with the large size of a phage genome, by using, for example, copies of a linearized capture vector (e.g., yeast artificial chromosome) and a set of linear bacteriophage genomic fragments with homologous "arms" that facilitate recombination.

[0008] Thus, various aspects of the invention provide methods that comprise introducing into yeast cells (a) copies of a linearized yeast artificial chromosome (YAC) and (b) a set of linear bacteriophage genomic fragments of defined sequence from at least two different types of bacteriophages, each genomic fragment comprising at each end a sequence of at least 20 contiguous nucleotides, wherein one of the two end sequences of each bacteriophage genomic fragment is homologous to only one other end sequence of an adjacent genomic fragment, and wherein the set of bacteriophage genomic fragments of defined sequence, when recombined, forms a nucleic acid encoding a viable recombinant bacteriophage with heterologous host recognition elements; and culturing the yeast cells to permit homologous recombination of the end sequences of the bacteriophage genomic fragments and the end sequences of the YAC, thereby producing a recombined YAC::phage construct that encodes a viable recombinant bacteriophage with heterologous host recognition elements.

[0009] In some embodiments, the methods comprise introducing into yeast cells (a) copies of a linearized yeast artificial chromosome (YAC) and (b) a set of linear bacteriophage genomic fragments of defined sequence from at least two different types of bacteriophages, each genomic fragment comprising at each end a sequence of at least 20 contiguous nucleotides, wherein one of the two end sequences of each bacteriophage genomic fragment is homologous to only one other end sequence of an adjacent genomic fragment, and wherein the set of bacteriophage genomic fragments of defined sequence, when recombined, forms a nucleic acid encoding a viable recombinant bacteriophage with heterologous tail fibers; and culturing the yeast cells to permit homologous recombination of the end sequences of the bacteriophage genomic fragments and the end sequences of the YAC, thereby producing a recombined YAC::phage construct that encodes a viable recombinant bacteriophage with heterologous tail fibers.

[0010] In some embodiments, the methods further comprise isolating and/or purifying the recombined YAC::phage construct.

[0011] In some embodiments, the copies of a linearized YAC comprise at each end a sequence of at least 20 contiguous nucleotides.

[0012] In some embodiments, the nucleic acids that encode a viable recombinant bacteriophage are formed by (i) a first subset of the genomic fragments of defined sequence that, when recombined, encode tail fibers from one type of bacteriophage and (ii) a second subset of the genomic fragments of defined sequence that, when recombined, encode a structure (e.g., capsid head, tail sheath) from a different type of bacteriophage.

[0013] In some embodiments, the methods further comprise expressing the YAC::phage construct to produce the viable recombinant bacteriophage.

[0014] In some embodiments, the set of bacteriophage genomic fragments of defined sequence is from at least one bacteriophage selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae and Guttavirus.

[0015] Various other aspects of the invention provide methods that comprise (a) introducing into yeast cells (i) copies of a linearized yeast artificial chromosome (YAC) comprising at one end a first end sequence of at least 20 contiguous nucleotides and at the other end a second end sequence of at least 20 contiguous nucleotides, and (ii) a first bacteriophage genomic fragment of defined sequence comprising at one end a third end sequence of at least 20 contiguous nucleotides and at the other end a fourth end sequence of at least 20 contiguous nucleotides, wherein the third end sequence is homologous to the first end sequence of the YAC, (iii) a second bacteriophage genomic fragment of defined sequence comprising at one end a fifth end sequence of at least 20 contiguous nucleotides and at the other end a sixth end sequence of at least 20 contiguous nucleotides, wherein the fifth end sequence is homologous to the end nucleotide sequence of the YAC, (iv) a third bacteriophage genomic fragment of defined sequence comprising at one end a seventh end sequence of at least 20 contiguous nucleotides and at the other end an eighth end sequence of at least 20 contiguous nucleotides, wherein the seventh end sequence is homologous to the fourth end sequence of the first bacteriophage genomic element, and the eighth end sequence is homologous to the sixth end sequence of the second bacteriophage genomic element, wherein the third bacteriophage genomic fragment comprises one bacteriophage genomic fragment or more than one bacteriophage genomic fragments that overlap by at least 20 contiguous nucleotides, wherein the first, second and third bacteriophage genomic fragments, when recombined, produce a nucleic acid encoding a viable recombinant bacteriophage with heterologous tail fibers, and wherein at least one of the bacteriophage genomic fragments is from one type of bacteriophage and at least one of the bacteriophage genomic fragments is from at least one different type of bacteriophage; and (b) culturing the yeast cells to permit homologous recombination of the end sequences of the bacteriophage genomic fragments and the end sequences of the YAC, thereby producing a recombined YAC::phage construct that encodes a viable recombinant bacteriophage with heterologous tail fibers.

[0016] In some embodiments, the methods further comprise isolating and/or purifying the recombined YAC::phage construct.

[0017] In some embodiments, at least one bacteriophage genomic fragment is from one type of bacteriophage and at least one bacteriophage genomic fragment is from a different type of bacteriophage.

[0018] In some embodiments, the bacteriophage genomic fragments, when recombined, produce a nucleic acid encoding tail fibers from one type of bacteriophage and a structure from a different type of bacteriophage.

[0019] In some embodiments, the methods further comprise expressing the YAC::phage construct to produce the viable recombinant bacteriophage.

[0020] In some embodiments, the first, second and/or third bacteriophage genome fragment of defined sequence is/are from at least one bacteriophage selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae, and Guttavirus.

[0021] Still other aspects of the invention provide yeast artificial chromosomes (YACs) that comprise a bacteriophage genome that encodes a viable bacteriophage with heterologous tail fibers.

[0022] In some embodiments, the bacteriophage genome comprises a set of overlapping bacteriophage genomic fragments of defined sequence from at least two different types of bacteriophages.

[0023] In some embodiments, the set of overlapping bacteriophage genomic fragments of defined sequence is from at least one bacteriophage selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae and Guttavirus.

[0024] Various aspects of the invention also provide compositions that comprise recombinant bacteriophages with heterologous tail fibers from at least two different types of bacteriophages.

[0025] In some embodiments, the heterologous tail fibers are from at least two bacteriophages selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae and Guttavirus.

[0026] The invention also provides, in some aspects, methods that comprise providing phagemids, each phagemid containing a nucleic acid that encodes a bacteriophage host recognition element, mutagenizing the nucleic acids that encode the bacteriophage host recognition elements to produce a phagemid library comprising a plurality of nucleic acids that encode a plurality of mutagenized bacteriophage host recognition elements, transforming bacterial cells with (a) lysogenic bacteriophages that are defective in the host recognition element and (b) the phagemid library, and isolating packaged phagemid particles.

[0027] In some embodiments, the methods further comprise infecting bacterial cells with the packaged phagemid particles.

[0028] In some embodiments, the methods further comprise culturing the bacterial cells infected with the phagemid particles.

[0029] In some embodiments, the methods further comprise isolating a nucleic acid that encodes a mutagenized bacteriophage host recognition element from the bacterial cells infected with the phagemid particles.

[0030] In some embodiments, the methods further comprise characterizing the nucleic acid that encodes the mutagenized bacteriophage host recognition element.

[0031] In some embodiments, the characterizing comprises amplifying from the bacterial cells infected with the phagemid particles a nucleic acid that encodes the mutagenized bacteriophage host recognition element and a nucleic acid that encodes a bacterial 16S sequence to produce a first amplified nucleic acid fragment and a second amplified nucleic acid fragment, respectively.

[0032] In some embodiments, the methods further comprise fusing the first amplified nucleic acid fragment and the second amplified nucleic acid fragment to produce a single amplicon.

[0033] In some embodiments, the methods further comprise sequencing the amplicon to identify bacterial cell host ranges of the mutagenized bacteriophage host recognition element.

[0034] In some embodiments, at least one of the bacteriophage host recognition element is from at least one bacteriophage selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae and Guttavirus.

[0035] In some embodiments, at least one of the bacteriophage host recognition elements is a tail fiber.

BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The accompanying drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component illustrated in various figures is represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing.

[0037] FIG. 1 depicts a yeast-based platform of the present disclosure for engineering recombinant bacteriophages with heterologous host recognition elements.

[0038] FIG. 2 depicts a yeast-based platform of the present disclosure for capturing a recovering wild-type bacteriophage species.

[0039] FIG. 3 depicts a plaque formation assay (left) and shows an image of a plaque formation assay using T3 and T7 phage on Escherichia coli (E. coli) strains BL21, DH5.alpha., DH10B, BW25113 and MG1655 (right).

[0040] FIG. 4 shows a graph of data from an adsorption assay with T3 phage and E. coli BL21 BW25113 and MG1655.

[0041] FIG. 5A depicts PCR fragments for engineering a synthetic T3 phage with a T3 tail fiber in a yeast artificial chromosome (YAC) (top) and PCR fragments for engineering a synthetic T3 phage with a T7 tail fiber in a YAC (bottom). Fragments 1, 2, 3 and 4 are from the T3 phage genome, and fragment 6 is from the T7 genome. FIG. 5B shows an image of an electrophoresis gel with PCR-amplified fragments from T3 and T7 phage genome. FIG. 5C shows synthetic T7 phages with a T3 tail fibers. As shown in the gel at the bottom left, nine PCR fragments (numbered 1-9), in total, were generated for the three engineered phages. For each T7 phage with T3 tail fibers, six PCR fragments (numbered 1-3, 5, 6 and 9 for T7.sub.T3gp17, and 1-3 and 7-9 for T7.sub.T3gp17-partial) were co-transformed and assembled in yeast. The YAC::phage was extracted and transformed in the bacterial strain designated 10G. As a control, a T7 wild-type phage (T7.sub.WT) was assembled from four PCR products (1-4).

[0042] FIG. 6A shows images of plaque formation assays with engineered bacteriophage and E. coli strains BL21, DH5.alpha., DH10B, BW25113 and MG1655. FIG. 6B shows images of plaque formation assays with engineered bacteriophage and bacterial strains BL21, DH5.alpha., 10G, BW25113, MG1655, Klebsiella sp. 390, ECOR4, ECOR13 and ECOR16.

[0043] FIG. 7 shows a vector map of Enterobacteria phage T7 (SEQ ID NO:1).

[0044] FIG. 8 shows a vector map of Enterobacteria phage SP-6 (SEQ ID NO:2).

[0045] FIG. 9 shows a vector map of Enterobacteria phage K1-5 (SEQ ID NO:3).

[0046] FIG. 10 shows a vector map of pRS415 (SEQ ID NO:34).

[0047] FIG. 11A shows T7 tail complexes. A tail structure contains two components: a tubular structure and tail fibers. The tubular structure contain an adaptor (gp11) and a nozzle (gp12). Tail fiber gp17 interacts with the interface between gp11 and gp12. FIG. 11B shows a schematic illustration of the combination of head and tail between T7 and K11 phages. T7 head and K11 tail result in T7K11gp1-12-17 and K11 head and T7 tail result in K11T7gp11-12-17.

[0048] FIG. 12A shows a plaque assay whereby synthetic T3 with R tail fibers (T3.sub.Rgp17) is capable of infecting Escherichia coli strain BL21 and Yersinia strains IP2666 and YPIII. FIG. 12A also shows that synthetic T7 with K11 tail fibers (T7.sub.K11gp11-12-17) is capable of infecting Klebsiella and that synthetic K11 with T7 tail fibers (K11.sub.T7gp11-12-17) is capable of infecting BL21. FIG. 12B shows the percent adsorption efficiency of T7.sub.WT, K11.sub.WT, T7.sub.K11gp11-12-17, and K11.sub.T7gp11-12-17.

[0049] FIG. 13 shows a schematic summary of synthetic phages engineered using methods of the present disclosure.

DETAILED DESCRIPTION OF THE INVENTION

[0050] Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome and may have relatively simple or elaborate structures. As used herein, the term "bacteriophage" includes naturally-occurring and recombinant bacteriophages, unless otherwise indicated. A "naturally-occurring" bacteriophage is a phage isolated from a natural or human-made environment that has not been modified by genetic engineering. A "recombinant bacteriophage" is a phage that comprises a genome that has been genetically modified by insertion of a heterologous nucleic acid sequence into the genome. In some embodiments, the genome of a naturally-occurring phage is modified by recombinant DNA technology to introduce a heterologous nucleic acid sequence into the genome at a defined site.

[0051] Bacteriophage genomes may encode as few as four genes, and as many as hundreds of genes. A bacteriophage particle recognizes and binds to its host bacterial cell through its tail fibers and/or other bacteriophage host recognition elements (e.g., tail spikes), causing DNA in the head of the phage to be ejected into the cytoplasm of the bacterial cell where the bacteriophage replicates using either a lytic cycle, which typically results in bacterial cell lysis, or a lysogenic (non-lytic) cycle, which leaves the bacterial cell intact. Differences in bacteriophage host recognition mainly reflect differences in bacterial cell surface receptors. Bacteriophage attachment to bacterial cells requires the binding of host recognition elements to bacterial receptor molecules, and it is typically the host recognition element (e.g., tail fiber) that determines the host range (e.g., different species of host bacterial cells). Thus, altering (e.g., changing or mutating) the host recognition elements of a bacteriophage, in turn, can alter bacteriophage infectivity. Provided herein are methods that can be used to achieve artificial control of bacteriophage infectivity, thereby altering and, in some instances, expanding the range of phage host cells for particular recombinant bacteriophages. As used herein, a "phage host cell" is a cell that can be infected by a phage to yield progeny phage particles.

Bacteriophages

[0052] Bacteriophages are obligate intracellular parasites that multiply inside bacteria by making use of some or all of the host biosynthetic machinery. Though different phages may contain different materials, they all contain nucleic acid and protein, and may be covered by a lipid membrane. A bacteriophage genome typically consists of a single, linear or circular, double- or single-stranded nucleic acid. Depending on the phage, the nucleic acid can be either DNA or RNA. Thus, in some embodiments, a bacteriophage of the invention contains DNA, while in other embodiments, a bacteriophage contains RNA. The size of the nucleic acid may vary depending on the phage. A genome of the simplest phages are only a few thousand nucleotides in size, while a genome of more complex phages may be more than 100,000 nucleotides in size, and in rare instances, more than 1,000,000 nucleotides. The number of different kinds of protein and the amount of each kind of protein in the bacteriophage particle may vary depending on the phage. The proteins function in infection and to protect the nucleic acid from nucleases in the environment.

[0053] Many bacteriophages range in size from 24-200 nm in diameter. Those having a capsid head may be composed of many copies of one or more different proteins. The nucleic acid is located in the capsid head, which acts as a protective covering for the nucleic acid. For filamentous phage, without capsid heads, the nucleic acid is simply coated with proteins. Many phages have tails attached to the capsid head. The tail is a hollow tube through which the nucleic acid passes during infection. The size of the tail can vary, and in more complex phages, the tail is surrounded by a contractile sheath which contracts during infection of the phage host bacterium. At the end of the tail, phages have a base plate and one or more tail fibers attached to it. The base plate and tail fibers are involved in the adsorption of the phage to the host cell. The main determinant of adsorption and specificity toward bacteria for most phages lies in small appendages surrounding the tail known as tail fibers or tail spikes, depending on their morphology. For phages of the T7 family, the host determinant is encoded by gene gp17, and the mature virus typically has 6 tail fibers each composed of a trimer of Gp17 (Steven, A C et al. J Mol Biol 200, 351-365 (1988)).

[0054] Some bacteriophage tails may be long, flexible and non-contractile (e.g., Siphoviridae such as lambda). The tail may be connected to the head via a portal complex that may or may not carry side tail fibers. Host recognition proceeds though the tip of the tail fibers (adhesin), thinner fibers located at the very tip of the tail, the tail baseplate, or any combination of the foregoing. Other bacteriophage tails may be long, rigid and contractile (e.g., Myoviridae such as T4, Mu). The tail may have a contractile sheath surrounding the tubular structure of the tail. It may also be attached to the head via a portal complex that may also carry side tail fibers. Host recognition is assumed to proceed primarily through the tip of the tail fiber (e.g., adhesin) or through other recognition elements located at the tip of the tail itself, in the baseplate. Yet other bacteriophage tails may be short, rigid and non-contractile (e.g., Podoviridae such as P22 and T7). The tail may be almost non-existent, but the portal complex is still present. In some instances, a bacteriophage may harbor tail fibers or tail spikes on its portal that are responsible for host recognition.

[0055] The first step in the bacteriophage infection process is the adsorption of the phage to the cell membrane. This step is mediated by the tail fibers and/or other bacteriophage host recognition elements and is reversible. For example, the tail fibers attach to specific receptors on the cell and the host specificity of the phage (e.g., the bacteria that it is able to infect) is usually determined by the type of phage tail fibers. The nature of the bacterial receptor varies for different bacteria. Examples of receptors include proteins on the outer surface of the cell, lipopolysaccharide (LPS), pili and lipoprotein.

[0056] The attachment of the bacteriophage to the cell through the tail fibers is typically weak and reversible. The irreversible binding of the phage to the cell results in the contraction of the sheath, if present, and delivery of the hollow tail fiber through the bacterial envelope. The nucleic acid from the capsid head then passes through the hollow tail and enters the cell.

[0057] The bacteriophages of the invention may be lytic (or virulent) or non-lytic (or lysogenic or temperate). Lytic bacteriophages are phages that can only multiply on bacteria and kill the cell by lysis at the end of the life cycle. Lytic phage, in some embodiments, may be enumerated by a plaque assay. A plaque is a clear area that results in a lawn of bacterial grown on a solid media from the lysis of bacteria. The assay may be performed at a low enough concentration of phage that each plaque arises from a single infectious phage. The infectious particle that gives rise to a plaque is referred to as a PFU (plaque forming unit).

[0058] Lysogenic bacteriophages are those that can either multiply through the lytic cycle or enter a quiescent state in the cell. In this quiescent state, most of the phage genes are not transcribed; the phage genome exists in a repressed state. The phage DNA in this repressed state is referred to as a prophage because it has the potential to produce phage. In most cases, the phage DNA actually integrates into the host chromosome and is replicated along with the host chromosome and passed on to the daughter cells. The cell harboring a prophage is not adversely affected by the presence of the prophage, and the lysogenic state may persist indefinitely. The cell harboring a prophage is referred to as a lysogen.

[0059] Examples of bacteriophage for use in accordance with the invention include, without limitation, those of the order Myoviridae (T4-like virus; P1-like viruses; P2-like viruses; Mu-like viruses; SPO1-like viruses; phiH-like viruses); Siphoviridae (.lamda.-like viruses, .gamma.-like viruses, T1-like viruses; T5-like viruses; c2-like viruses; L5-like viruses; .psi.M1-like viruses; phiC31-like viruses; N15-like viruses); Podoviridae (T7-like virus; phi29-like viruses; P22-like viruses; N4-like viruses); Tectiviridae (Tectivirus); Corticoviridae (Corticovirus); Lipothrixviridae (Alphalipothrixvirus, Betalipothrixvirus, Gammalipothrixvirus, Deltalipothrixvirus); Plasmaviridae (Plasmavirus); Rudiviridae (Rudivirus); Fuselloviridae (Fusellovirus); Inoviridae (Inovirus, Plectrovirus, M13-like viruses, fd-like viruses); Microviridae (Microvirus, Spiromicrovirus, Bdellomicrovirus, Chlamydiamicrovirus); Leviviridae (Levivirus, Allolevivirus), Cystoviridae (Cystovirus), Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae, and Guttavirus. Such phages may be naturally occurring or engineered.

[0060] In some embodiments, a bacteriophage genome may comprise at least 5 kilobases (kb), at least 10 kb, at least 15 kb, at least 20 kb, at least 25 kb, at least 30 kb, at least 35 kb, at least 40 kb, at least 45 kb, at least 50 kb, at least 55 kb, at least 60 kb, at least 65 kb, at least 70 kb, at least 75 kb, at least 80 kb, at least 85 kb, at least 90 kb, at least 95 kb, at least 100 kb, at least 105 kb, at least 110 kb, at least 115 kb, at least 120 kb, at least 125 kb, at least 130 kb, at least 135 kb, at least 140 kb, at least 145 kb, at least 150 kb, at least 175 kb, at least 200 kb, at least 225 kb, at least 250 kb, at least 275 kb, at least 300 kb, at least 325 kb, at least 350 kb, at least 325 kb, at least 350 kb, at least 375 kb, at least 400 kb, at least 425 kb, at least 450 kb, at least 475 kb, at least 500 kb, or more.

[0061] The bacteriophages of the invention infect bacteria. Bacteria are small (typical linear dimensions of around 1 micron), non-compartmentalized, with circular DNA and ribosomes of 70S. As used herein, the term "bacteria" encompasses all variants of bacteria, including endogenous bacteria. "Endogenous" bacteria naturally reside in a closed system (e.g., bacterial flora) and are typically non-pathogenic. The invention contemplates bacteriophages that infect non-pathogenic and/or pathogenic bacteria. The bacteriophages of the invention may infect bacterial cells of the subdivisions of Eubacteria. Eubacteria can be further subdivided into Gram-positive and Gram-negative Eubacteria, which depend on a difference in cell wall structure. Also included herein are those classified based on gross morphology alone (e.g., cocci, bacilli). In some embodiments, the bacterial cells are Gram-negative cells, and in some embodiments, the bacterial cells are Gram-positive cells. Examples of bacterial cells of the invention include, without limitation, Yersinia spp., Escherichia spp., Klebsiella spp., Bordetella spp., Neisseria spp., Aeromonas spp., Franciesella spp., Corynebacterium spp., Citrobacter spp., Chlamydia spp., Hemophilus spp., Brucella spp., Mycobacterium spp., Legionella spp., Rhodococcus spp., Pseudomonas spp., Helicobacter spp., Salmonella spp., Vibrio spp., Bacillus spp., Erysipelothrix spp., Salmonella spp., Streptomyces spp., Bacteroides spp., Prevotella spp., Clostridium spp., Bifidobacterium spp., or Lactobacillus spp. In some embodiments, the bacterial cells are Bacteroides thetaiotaomicron, Bacteroides fragilis, Bacteroides distasonis, Bacteroides vulgatus, Clostridium leptum, Clostridium coccoides, Staphylococcus aureus, Bacillus subtilis, Clostridium butyricum, Brevibacterium lactofermentum, Streptococcus agalactiae, Lactococcus lactis, Leuconostoc lactis, Actinobacillus actinobycetemcomitans, cyanobacteria, Escherichia coli, Helicobacter pylori, Selnomonas ruminatium, Shigella sonnei, Zymomonas mobilis, Mycoplasma mycoides, Treponema denticola, Bacillus thuringiensis, Staphylococcus lugdunensis, Leuconostoc oenos, Corynebacterium xerosis, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus acidophilus, Streptococcus Enterococcus faecalis, Bacillus coagulans, Bacillus ceretus, Bacillus popillae, Synechocystis strain PCC6803, Bacillus liquefaciens, Pyrococcus abyssi Selenomonas nominantium, Lactobacillus hilgardii, Streptococcus ferus, Lactobacillus pentosus, Bacteroides fragilis, Staphylococcus epidermidis, Zymomonas mobilis, Streptomyces phaechromogenes, or Streptomyces ghanaenis. Thus, the bacteriophage of the invention may target (e.g., specifically target) a bacterial cell from any one or more of the foregoing genus and/or species of bacteria. In some embodiments, the bacteriophage may target E. coli strains BL21, DH5.alpha., DH10B, BW25113, Nissle 1917 and/or MG1655 and/or derivatives of any of the foregoing strains (e.g., a modified strain with, for example, a mutation, insertion and/or plasmid).

[0062] In some embodiments, the bacteriophages of the invention infect bacteria of a phyla selected from Actinobacteria, Aquificae, Armatimonadetes, Bacteroidetes, Caldiserica, Chlamydiae, Chloroflexi, Chrysiogenetes, Cyanobacteria, Deferribacteres, Deinococcus-Thermus, Dictyoglomi, Elusimicrobia, Fibrobacteres, Firmicutes (e.g., Bacillus, Listeria, Staphylococcus), Fusobacteria, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria (e.g., Acidobacillus, Aeromonas, Burkholderia, Neisseria, Shewanella, Citrobacter, Enterobacter, Erwinia, Escherichia, Klebsiella, Kluyvera, Morganella, Salmonella, Shigella, Yersinia, Coxiella, Rickettsia, Legionella, Avibacterium, Haemophilus, Pasteurella, Acinetobacter, Moraxella, Pseudomonas, Vibrio, Xanthomonas), Spirochaetes, Synergistets, Tenericutes (e.g., Mycoplasma, Spiroplasma, Ureaplasma), Thermodesulfobacteria and Thermotogae.

[0063] The invention also contemplates, in various aspects and embodiments, substituting bacteriophages for archaeophages (i.e., viruses that infect archaea such as, e.g., (.phi.H viruses). Thus, in some embodiments, the phages are able to productively infect archaea. In some embodiments, the archaea is a Euryarcheota. In some embodiments the archaea is a Crenarcheota.

Engineering Recombinant Bacteriophages with Heterologous Tail Fibers

[0064] Recombinant bacteriophages of the invention can be engineered by introducing genomic fragments from at least two different bacteriophage genomes into a replicating capture vector with a selectable marker. In some embodiments, the heterologous host recognition particles of the recombinant bacteriophage are encoded by genomic fragments from one type of bacteriophage, while all or most other structures (e.g., capsid head, tail sheath, base plate) are encoded by genomic fragments from a different type of bacteriophage. In general, copies of the linearized capture vector (e.g., YAC) and the set of linear bacteriophage genomic fragments of defined sequence are co-transformed into competent host cells (e.g., yeast cells) and plated on selective media. Cell colonies that grow on the selective media are presumed to contain circularized vector::phage constructs resulting from homologous recombination among the linear bacteriophage genomic fragments and between the linear bacteriophage genomic fragments and the linearized capture vector. The cell colonies are then screened for the presence of junctions between vector DNA and phage DNA, the presence of which indicates successful cloning of the set of linear bacteriophage genomic fragments into the capture vector. Successful cloning results in a recombinant circular nucleic acid molecule that encodes a viable recombinant bacteriophage with heterologous host recognition elements (e.g., heterologous tail fibers).

[0065] Phage Genome Isolation

[0066] Any suitable method may be used to isolate phage genomes from phage cultures and/or isolated phage and/or concentrated phage preparations. The methods of the invention, in some embodiments, include the use of phage genomes from at least two different types of bacteriophage with a different, or overlapping, host ranges. Examples, of methods that may be used in accordance with the invention to isolate phage genomes include, without limitation, column-based, polyethylene glycol (PEG)-based, filter-based and cesium chloride centrifugation methods. In some embodiments, a phage genome may be isolated by simply boiling phage lysates as a dilution (e.g., 10-fold dilution) in buffer (e.g., TE buffer).

[0067] In some embodiments of the invention, a column-based method is used to isolate phage genomes. For example, high-titer lysates of a phage culture may be further concentrated via chromatography based on charge and/or affinity, permitting the concentration of large volumes of lysate into very small volumes. Passing the phages over a column, and then eluting into a small volume provides the material for DNA-harvesting of phages for further genome manipulation.

[0068] In some embodiments of the invention, a PEG-based method is used to isolate phage genomes. For example, the presence of high-concentrations of polyethylene glycol permits precipitation of active phage particles from a lower-titer, high volume of phage material.

[0069] In some embodiments of the invention, a filter-based method is used to isolate phage genomes. For example, filtering lysates to remove large cell debris, followed by filtration in the 100 kDa size range permits the retention of phage particles, while losing water and salts in the phage lysate preparation.

[0070] In some embodiments of the invention, a cesium chloride centrifugation method is used to isolate phage genomes. For example, concentrated lysates may be purified by treating them with DNases to remove contaminating host DNA, followed by centrifugation in a cesium chloride gradient to purify the phage particles away from the cell debris.

[0071] Any suitable method may be used to purify phage genomes. In some embodiments, regardless of the purification method, phage lysates may be treated with proteases and chloroform to remove the phage coats, followed by either column-based DNA purification or ethanol precipitation of the recovered DNA. DNA recovered at this step is typically ready for further capture and manipulation.

[0072] If the bacteriophage genomic sequence is unknown, the invention contemplates, in some embodiments, methods of generating a complete sequence. For example, next generation sequencing techniques may be used to generate large amounts of data (e.g., contigs) that can be used to assemble contiguous pieces of phage sequence. This sequence is often not sufficient to close an entire phage genome with a single pass, and thus remaining gaps may be filled using PCR-based techniques. Primers designed to anneal to the ends of contigs can be used in combination to amplify the phage genomic DNA. Only primers from contigs that are adjacent to each other will be amplify as a product. These PCR products can be sequenced by traditional Sanger sequencing to close the gaps between contigs.

[0073] Modified Sanger sequencing may also be used to directly sequence phage genomic DNA. This technique can be used, in some embodiments, to sequence the ends of the phage given that PCR cannot be used to capture this final sequence. This will complete the phage genomic sequence.

[0074] Bacteriophage Genomic Fragments

[0075] As used herein, a "genomic fragment" refers to an oligonucleotide isolated from, or synthesized based on, a bacteriophage genome. For brevity, genomic fragments will be referred to in the context of being isolated from a bacteriophage genome; however, any of the genomic fragments for use in accordance with the invention may be synthesized to produce an oligonucleotide that is homologous to (e.g., the same as) an oligonucleotide isolated from a genome of a particular type of bacteriophage. Genomic fragments include, for example, genes, gene fragments, gene cassettes (e.g., more than one gene), origins of replication, and phage packaging signals. In some embodiments, a genomic fragment may have a length of about 50 nucleotides to about 10,000 nucleotides. For example, a genomic fragment may have a length of about 50 nucleotides to about 5,000 nucleotides, about 50 to about 1,000 nucleotides, about 1,000 nucleotides to about 10,000 nucleotides, about 5,000 nucleotides to about 10,000 nucleotides. In some embodiments, a genomic fragment may have a length of about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900, 3000, 3100, 3200, 3300, 3400, 3500, 3600, 3700, 3800, 3900, 4000, 5000, 5100, 5200, 5300, 5400, 5500, 5600, 5700, 5800, 5900, 6000, 6100, 6200, 6300, 6400, 6500, 6600, 6700, 6800, 6900, 7000, 7100, 7200, 7300, 7400, 7500, 7600, 7700, 7800, 7900, 7000, 7100, 7200, 7300, 7400, 7500, 7600, 7700, 7800, 7900, 8000, 8100, 8200, 8300, 8400, 8500, 8600, 8700, 8800, 8900, 9000, 9100, 9200, 9300, 9400, 9500, 9600, 9700, 9800, 9900 or 10000 nucleotides. Other embodiments contemplate larger genomic fragments. Thus, in some embodiments, a genomic fragment may have a length of about 10,000 nucleotides to about 15,000 nucleotides, or more. For example, a genomic fragment may have a length of about 10000, 11000, 12000, 13000, 14000 or 15000 nucleotides, or more.

[0076] As used herein, "a set of linear bacteriophage genomic fragments of defined sequence" refers to a set of genomic fragments that, when combined to form a single contiguous nucleic acid, encodes a full length hybrid phage genome, or as much of a hybrid phage genome that is necessary and sufficient to encode a fully functional (e.g., viable and infectious) phage. As used herein, an "infectious" phage refers to a phage that can adsorb to and inject its nucleic acid into a bacterial cell. Thus, a bacteriophage is considered to "infect" a host cell when it adsorbs to and injects its nucleic acid into the cell. In some embodiments, an infectious phage can productively infect, replicate and burst a particular host cell. A "hybrid phage genome," as used herein, refers to a genome comprising genomic fragments from genomes of at least two different types of bacteriophages.

[0077] A fully functional phage may require the following: (1) the ability to take control of the host in order to produce phage; (2) an origin of replication and associated replication functions; (3) a complete set of genes permitting capsid assembly; (4) a complete set of genes permitting tail assembly; (5) structures (e.g., tail fibers or tail spikes) for bacteriophage adsorption to the host cell; and/or (6) packaging functions. In some instances, a fully functional phage may also require functions to counteract host defenses such as restriction (e.g., T7 gp0.3, T4 IPI, DNA methylases) or abortive infection (e.g., T4 dmd, T3 gp1.2).

[0078] In some embodiments, bacteriophage can use its own transcriptional and translational machinery to produce phage, while in other embodiments, the bacteriophage may utilize the host cell's transcriptional and translational machinery.

[0079] In some embodiments, associated replication functions may be provided by the host cell.

[0080] In some embodiments, a fully functional bacteriophage may require tail fibers to adsorb to a host cell. For example, T7 and T4 bacteriophages use tail fibers to adsorb to host cells. In other embodiments, a fully functional bacteriophage may require tail spikes to adsorb to a host cell. For example, P22 and K1-5 bacteriophages use tail spikes to adsorb to host cells. In yet other embodiments, a fully functional bacteriophage may require dispensable tail fibers to adsorb to a host cell. For example, lambda bacteriophages use dispensable tail fibers to adsorb to host cells.

[0081] Packaging may proceed through various mechanisms depending on the bacteriophage. Some bacteriophages use a site-specific nuclease to initiate cleavage from concatemerized genomes during replication (e.g., COS phages, lambda). Other bacteriophages use a partially site specific nuclease to initiate packaging (e.g., the first cut occurs at a predefined site along the phage genome). The bacteriophages then package, through a "headful mechanism," phage genome monomers from a concatemer generated during replication. The headful mechanism entails the bacteriophage injecting as much DNA inside the capsid as can fit, cutting the DNA, and then continuing the packaging reaction in another capsid (e.g., P22, T4). Still other bacteriophages have long terminal repeats (LTRs) with a packaging enzyme that will recognize two contiguous repeats, cut between them and initiate packaging from the cut site until it encounters another occurrence of two contiguous LTRs (e.g., T7, K1-5).

[0082] The linear genomic fragments may be synthesized or amplified (e.g., via polymerase chain reaction (PCR)) from isolated and/or purified bacteriophage genome(s). Sets of PCR primers may be chosen using the following parameters: (1) the set of amplified fragments must span all the genes necessary for a viable phage, and (2) there must be at least 20 base pairs (bp) of homology between each amplified fragment to be assembled (e.g., recombined). In some embodiments, a set of linear bacteriophage fragments is synthesized de novo.

[0083] Thus, the set of linear bacteriophage genomic fragments of defined sequences is designed such that each genomic fragment comprises at each end a sequence of at least 20 contiguous nucleotides (referred to herein as an "end sequence"), wherein one of the two end sequences of each bacteriophage genomic fragment is homologous to only one other end sequence of an adjacent genomic fragment. In this way, the genomic fragments can be pieced, or "stitched," together based on homology to form a nucleic acid encoding, in some embodiments, a full length hybrid (or recombinant) phage genome. In some embodiments, each genomic fragment comprises at each end a sequence of at least 30 contiguous nucleotides, at least 35 contiguous nucleotides, at least 40 contiguous nucleotides, at least 45 contiguous nucleotides, at least 50 contiguous nucleotides, or more.

[0084] The set of linear bacteriophage genomic fragments of defined sequence and copies of the linearized capture vector are co-transformed into competent host cells. A "host cell," as used herein, refers to a cell into which a recombinant nucleic acid, such as a recombinant vector, has been introduced or produced. Common hosts include, for example, bacteria (e.g., Escherichia coli, Bacillus subtilis), yeast (e.g., Saccharomyces cerevisiae such as BY4741) and various eukaryotic cell lines. In some embodiments, the set of linear bacteriophage genomic fragments of defined sequence and copies of linearized YAC are co-transformed into competent yeast cells. The set of genomic fragments and linearized capture vector may be combined with an excess of the vector prior to transformation. For example, in some embodiments, an excess of about 50 ng to about 500 ng (e.g., an excess of 50 ng, 100 ng, 200 ng, 250 ng, or 500 ng) of linearized capture vector is used. In some embodiments, an excess of about 100 ng to about 300 ng of linearized capture vector is used.

[0085] Heterologous Tail Fibers

[0086] The invention contemplates, in some embodiments, tuning bacteriophage host range by engineering recombinant bacteriophage having heterologous tail fibers. As discussed elsewhere herein, host cell specificity of the phage is typically determined by the tail fiber(s). By altering (e.g., swapping and/or mutating) tail fibers, or portions of tail fibers, of a host bacteriophage, the host range, in some embodiments, can be altered (e.g., expanded).

[0087] A "host bacteriophage," as used herein, refers to the type of bacteriophage (e.g., T3, T4, T5, T7, K1F, K11, SP6) from which genomic fragments encoding the capsid head (and optionally other non-tail fiber structures) are isolated. As used herein, a "heterologous tail fiber" refers to a tail fiber that does not naturally occur on the host bacteriophage. For example, a heterologous tail fiber may be encoded by genomic fragment(s) isolate from the genome of a type of bacteriophage that is different from the host bacteriophage. Thus, in some embodiments, a recombinant bacteriophage having heterologous tail fibers may have a capsid head from a T7 phage and tail fibers, or portions thereof, from any one or more of T3, T4, T5, K1F, K11, or SP6 phage(s). In some embodiments, a heterologous tail fiber is not a natural phage sequence, while in other embodiments, it is a natural phage sequence, albeit from a different type of phage.

[0088] In some embodiments, a recombinant bacteriophage with heterologous tail fibers is encoded by a set of linear bacteriophage genomic fragments of defined sequence that is isolated from the genomes of at least two different types of bacteriophage. For example, a recombinant bacteriophage of the invention may contain a capsid head and tail sheath (and/or other phage structures) encoded by a subset genomic fragments isolated from the genome of one type of bacteriophage and tail fibers encoded by a subset genomic fragments isolated from the genome of another type of bacteriophage.

[0089] In other embodiments, a recombinant bacteriophage with heterologous tail fibers is encoded by a set of linear bacteriophage genomic fragments of defined sequence that is isolated from the genomes of at least three, or more, different types of bacteriophage. For example, a recombinant bacteriophage of the invention may contain a capsid head (and/or other phage structures) encoded by a subset of genomic fragments isolated from the genome of one type of bacteriophage (e.g., T3 phage) and tail fibers encoded by multiple subsets genomic fragments, each of the multiple subsets isolated from the genome of different types of bacteriophages (e.g., T4, T5, T7, K1F, K11, or SP6 phage).

[0090] Tail fiber proteins typically contain antigenicity determinants and host range determinants. In some embodiments, a heterologous tail fiber may be encoded by a set of genomic fragments isolated from one type of bacteriophage. In other embodiments, the set of genomic fragments may contain subsets of genomic fragments isolated from genomes of different types of bacteriophages. For example, conserved regions of a tail fiber may be encoded by genomic fragments isolated from the genome of the host bacteriophage, while host range determinant regions may be encoded by genomic fragments isolated from the genome of a different type of bacteriophage.

[0091] In some embodiments, the recombinant bacteriophages of the invention comprise tail fibers that are completely heterologous. That is, the whole tail fiber is encoded by a nucleic acid that is not present in the host bacteriophage. For example, the heterologous tail fiber of a T3 host bacteriophage may be encoded by gene 17, which is isolated from or stitched together from genomic fragments isolated from T7 phage. Likewise, the heterologous tail fiber of a T7 host bacteriophage may be encoded by gene 17 from T3 phage. In some embodiments, the recombinant bacteriophages of the invention comprise tail fibers that are partially heterologous. That is, only a part of the tail fiber is encoded by a nucleic acid that is not present in the host bacteriophage. For example, the partially heterologous tail fiber of a T3 host bacteriophage may be encoded by a recombinant nucleic acid comprising genomic fragments from T3 phage and genomic fragments from T7. Herein, "partially heterologous tail fibers" are considered to be encompassed by the term "heterologous tail fibers." In some embodiments, at least 10% of the nucleic acid sequence encoding a partially heterologous tail fiber is present in the host bacteriophage. For example, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or at least 99% of the nucleic acid sequence encoding a partially heterologous tail fiber is present in the host bacteriophage. In other embodiments, at least 10% of the nucleic acid sequence encoding a partially heterologous tail fiber is not present in the host bacteriophage. For example, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or at least 99% of the nucleic acid sequence encoding a partially heterologous tail fiber is from a bacteriophage that is not the host bacteriophage.

[0092] Capture Vectors

[0093] As used herein, a "capture vector" refers to a nucleic acid molecule into which a phage genome has been inserted. Examples of capture vectors for use in accordance with the invention include bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). Bacteriophage for which the genome sequence is known permits recombination of the genome into, for example, a circular vector, such as a YAC, using double strand break repair or other modes of recombination in, for example, yeast such as Saccharomyces cerevisiae.

[0094] The capture vectors of the invention contain selectable markers. Selectable markers for use herein include, without limitation, genes encoding proteins that increase or decrease either resistance or sensitivity to antibiotics (e.g., ampicillin resistance genes, kanamycin resistance genes, neomycin resistance genes, tetracycline resistance genes and chloramphenicol resistance genes) or other compounds, genes encoding enzymes with activities detectable by standard assays known in the art (e.g., .beta.-galactosidase, luciferase or alkaline phosphatase), and genes that visibly affect the phenotype of transformed or transfected cells, hosts, colonies, or plaques (e.g., green fluorescent protein). Other selectable markers may be used in accordance with the invention.

[0095] The capture vectors are first linearized before inserting a set of linearized bacteriophage genomic fragments of defined sequence. The capture vectors may be linearized by any method known in the art such as, for example, restriction digest.

[0096] Phage Genome Capture and Characterization

[0097] Any suitable transformation method may be used. The method may depend on the host cell. For example, in some embodiments, a lithium acetate transformation method is used (see e.g., Finlayson, S. D. et al. Biotechnology Techniques, 5(1), 13-18 (1991)) to transform yeast cells, followed by heat shock.

[0098] Transformed host cells (also referred to herein as "transformants") may be plated on any suitable selective media. The selective media will depend, in part, on the host cell and the selectable marker of the capture vector. For example, if an ampicillin resistance gene is used as the selectable marker, transformants should be plated on selective media containing ampicillin. Only those transformants that contain a circularized recombinant vector that expresses an ampicillin resistance gene will grow.

[0099] Presence of a hybrid phage genome, or portions thereof, in a circularized recombinant vector may be confirmed using, for example, PCR-based methods, direct sequencing, restriction digestion or Phi29/sequencing readout. In some embodiments, primers may be used to enable PCR-based confirmation of a hybrid phage genome. For example, if one primer is specific for a portion of the capture vector just outside the region of the hybrid phage genome and another primer is specific for a portion of the hybrid phage genome, these primers should together amplify a band to verify that the proper hybrid phage genome and junctions are present in the circular recombinant vector. In some embodiments, the hybrid phage genome may be directly sequenced to confirm the presence of the hybrid phage DNA inside the vector. The presence of a hybrid phage genome may also be identified and characterized using restriction digestion and gel electrophoresis. In some embodiments, a DNA polymerase from bacteriophage Phi29 can be used to copy the hybrid phage genome in vitro. These substrates may then be used for transformation and sequencing. Further, amplification with Phi29 polymerase allows for analysis with restriction enzymes to identify Restriction Fragment Length Polymorphisms (RFLPs) for rapid whole genome analysis. These products can be run on agarose gels and analyzed by ethidium bromide staining.

[0100] Recombinant nucleic acids of the invention may be engineered using, for example, conventional molecular cloning methods (see, e.g., Current Protocols in Molecular Biology, Ausubel, F. M., et al., New York: John Wiley & Sons, 2006; Molecular Cloning: A Laboratory Manual, Green, M. R. and Sambrook J., New York: Cold Spring Harbor Laboratory Press, 2012; Gibson, D. G., et al., Nature Methods 6(5):343-345 (2009), the teachings of which relating to molecular cloning are herein incorporated by reference). The circular nucleic acids encoding the recombinant bacteriophage of the invention may be expressed in any suitable host cells.

Bacteriophage Host Range Engineering with Mutagenesis

[0101] The invention also provides high-throughput methods of tuning bacteriophage host range using nucleic acid mutagenesis and, in some embodiments, next-generation sequencing. Thus, various aspect of the invention are directed to methods that comprise providing phagemids, each phagemid containing a nucleic acid that encodes a bacteriophage host recognition element, mutagenizing the nucleic acids that encode the bacteriophage host recognition elements to produce a phagemid library comprising a plurality of nucleic acids that encode a plurality of mutagenized bacteriophage host recognition elements, transforming bacterial cells with (a) lysogenic bacteriophages that are defective in the host recognition element and (b) the phagemid library, and isolating packaged phagemid particles.

[0102] As used herein, a "phagemid" is a filamentous phage-derived vector containing the replication origin of a plasmid and the packaging site of a bacteriophage. Examples of phagemids that may be used in accordance with the invention include, without limitation, M13-derived phagemids containing the fl origin for filamentous phage packaging such as, for example, pBluescript II SK (+/-) and KS (+/-) phagemids, pBC SK and KS phagemids, pADL and P1-based phagemids (see, e.g., Westwater C A et al., Microbiology 148, 943-50 (2002); Kittleson J T et al., ACS Synthetoc Biology 1, 583-89 (2012); Mead D A et al., Biotechnology 10, 85-102 (1988)). Other phagemids may be used in accordance with the invention.

[0103] As used herein, a "bacteriophage host recognition element" refers to bacteriophage protein that confers phage host cell specificity. Alterations (e.g., mutations) in a bacteriophage host recognition element can alter the range of phage host cells for a particular host bacteriophage. Thus, in some embodiments, recombinant bacteriophage with heterologous or mutated host recognition elements, are able to infect phage host cells that the host bacteriophage otherwise would not be able to infect. Examples of bacteriophage host recognition elements include, without limitation, long side tail fibers (e.g., T4, lambda), short side tail fibers (e.g., T7, T3), tail spikes (e.g., P22, SP6, K1-5, K1E, K1F), short tail tip fibers (lambda), other parts of the baseplate (e.g., T4), or other host cell receptor recognition proteins. Specific non-limiting examples of bacteriophage host recognition elements include T4 gp37 (e.g., NCBI Accession No. NP_049863.1), gp37 (e.g., NCBI Accession No. AAC61976.1), gp38 (e.g., NCBI Accession No. AAC61977.1), Lambda J (e.g., NCBI Accession No. AAA96553.1), T7 gp17 (e.g., NCBI Accession No. NP_042005.1), T3 gp17 (e.g., NCBI Accession No. CAC86305.1), P22 gp9 (e.g., NCBI Accession No. NP_059644.1), SP6 gp46 (e.g., NCBI Accession No. NP_853609.1), K1-5 gp46 (e.g., NCBI Accession No. YP_654147.1), K1-5 gp47 (e.g., NCBI Accession No. YP_654148.1), K1F gp17 (e.g., NCBI Accession No. YP_338127.1), K1E gp47 (e.g., NCBI Accession No. YP_425027.1), K11 gp17 (e.g., NCBI Accession No. YP_002003830.1), phiSG-JL2 gp17 (e.g., NCBI Accession No. YP_001949790.1), phiIBB-PF7A gp17 (e.g., NCBI Accession No. YP_004306354.1), and 13a gp17 (e.g., NCBI Accession No. YP_002003979.1).

[0104] As used herein, the term "nucleic acid" refers to at least two nucleotides covalently linked together, and in some instances, may contain phosphodiester bonds (e.g., a phosphodiester "backbone"). Nucleic acids (e.g., components, or portions, of the nucleic acids) of the invention may be naturally occurring or engineered. Engineered nucleic acids include recombinant nucleic acids and synthetic nucleic acids. "Recombinant nucleic acids" may refer to molecules that are constructed by joining nucleic acid molecules and, in some embodiments, can replicate in a living cell. "Synthetic nucleic acids" may refer to molecules that are chemically or by other means synthesized or amplified, including those that are chemically or otherwise modified but can base pair with naturally occurring nucleic acid molecules. Recombinant and synthetic nucleic acids also include those molecules that result from the replication of either of the foregoing.

[0105] The nucleic acids may be single-stranded (ss) or double-stranded (ds), as specified, or may contain portions of both single-stranded and double-stranded sequence. The nucleic acid may be DNA, both genomic and cDNA, RNA or a hybrid, where the nucleic acid contains any combination of deoxyribo- and ribonucleotides, and any combination of bases, including uracil, adenine, thymine, cytosine, guanine, inosine, xanthine, hypoxanthine, isocytosine, and isoguanine.

[0106] Nucleic acids that encode bacteriophage host recognition elements can be mutagenized by any suitable methods. Examples of nucleic acid mutagenesis methods that can be used in accordance with invention include, without limitation, site-directed mutagenesis, PCR mutagenesis and insertional mutagenesis. Non-limiting examples of PCR mutagenesis include: (1) error-prone mutagenesis using manganese or cobalt to increase error rate during elongation, which yields randomly mutagenized host recognition elements; (2) 2-way PCR, which may be used to stitch two non-homologous sequences together; (3) site directed PCR mutagenesis, which uses primers that have selected mutations to amplify the gene of interest; and (4) semi-random primer directed mutagenesis, which uses primers that have randomized nucleotides (e.g., 1-40 nt) that introduce random mutations in a given location of a gene of interest.

[0107] In some embodiments, a "bank" of mutagenized DNA fragments (e.g., host recognition elements) may be obtained from a DNA synthesis company.

[0108] Any suitable transformation method (e.g., heat shock, electroporation) may be used to transform bacterial cells with the phagemid library and the lysogenic bacteriophages that are defective in the host recognition element.

[0109] As discussed elsewhere herein, lysogenic bacteriophages are those that can either multiply via the lytic cycle or enter a quiescent state in the cell. As used herein, lysogenic bacteriophages that are "defective in the host recognition element" are missing the particular host recognition element that is mutagenized in the phagemid library such that a phagemid copy complements the lysogenic bacteriophage.

[0110] As used herein, a "packaged phagemid particle" is a bacteriophage (e.g., lysogenic bacteriophage phage defective in the host recognition element) containing a phagemid (e.g., phagemid containing a mutagenized host recognition element).

[0111] After isolating the packaged phagemid particles, they may be used to infect bacterial cells. Examples of bacterial cell are provide elsewhere herein and include the following: Yersinia spp., Escherichia spp., Klebsiella spp., Bordetella spp., Neisseria spp., Aeromonas spp., Franciesella spp., Corynebacterium spp., Citrobacter spp., Chlamydia spp., Hemophilus spp., Brucella spp., Mycobacterium spp., Legionella spp., Rhodococcus spp., Pseudomonas spp., Helicobacter spp., Salmonella spp., Vibrio spp., Bacillus spp., Erysipelothrix spp., Salmonella spp., Streptomyces spp., Bacteroides spp., Prevotella spp., Clostridium spp., Bifidobacterium spp., or Lactobacillus spp. Bacterial cells infected with the phagemid particles can then be cultured using, for example, conventional bacterial cell culture methods for bacterial cell growth.

[0112] The nucleic acid that encodes the mutagenized bacteriophage host recognition elements can be isolated and/or purified from the bacterial cells infected with the phagemid particles using, for example, conventional nucleic acid methods (e.g., combine physical and chemical methods). Examples of nucleic acid extraction/purification methods include, without limitation, ethanol precipitation, phenol chloroform and column purification.

[0113] The nucleic acids may be characterized by any suitable means. For example, the nucleic acids may be characterized using a method referred to as "Stichseq" (Yu, et al. Nature Methods, 8, 478-480 (2011)). In some instances, the nucleic acids are amplified (e.g., by PCR) together with a nucleic acid that encodes a bacterial 16S sequence to produce a first amplified nucleic acid fragment and a second amplified nucleic acid fragment, respectively. The first and second amplified nucleic acid fragments can then be fused to produce a single amplicon, which can then be used to identify bacterial cell host ranges of the mutagenized bacteriophage host recognition element.

[0114] In some embodiments, host range recognition elements (e.g., tail fibers) may be mutated by site-directed mutagenesis and/or random mutagenesis by PCR and/or de novo nucleic acid synthesis.

[0115] In some embodiments, after capturing a phage genome in yeast, the host range determinant is replaced with yeast selection marker URA3. Mutated host range recognition elements may be added into yeast cell harboring YAC::phage::URA3. URA3 may be replaced with mutated host range determinant by homologous recombination and transformants selected by 5-FOA counter selection. It should be understood that each mutated host range recognition element has a homologous sequence of upstream and downstream regions of the target gene in the 5' and 3' terminal, respectively.

[0116] In some embodiments, a phage with a mutated host range recognition element is captured in one-step with gap-repair cloning. The recognition element may be generated through PCR mutagenesis or other well-known techniques.

Applications

[0117] The methods and compositions of the invention may be used in many different applications. For example, in some embodiments, provided herein are "phage cocktails" that comprise the recombinant bacteriophage with heterologous host recognition elements for use in, for example, phage therapy. Phage therapy is a therapeutic use of bacteriophages to treat pathogenic bacterial infections. Because the recombinant bacteriophage of the invention can be tuned to infect a broad range of host bacterial cells, they are a particularly useful alternative to conventional antibiotic therapy against, for example, multi-drug resistant bacteria. Thus, in some embodiments, the invention provides methods of treating bacterial infections (e.g., in humans or other animals) using recombinant bacteriophages with heterologous host recognition elements such as heterologous tail fibers. The methods may comprise administering to a subject with a bacterial infection a composition comprising a recombinant bacteriophage of the invention.

[0118] In some embodiments, the recombinant bacteriophages of the invention may be used as delivery vehicles to deliver, to bacterial cells, molecules (e.g., nucleic acids) of interest.

Compositions and Kits

[0119] Also provided herein are compositions and kits that comprise any one or more of the bacteriophages, phagemids, nucleic acids and/or libraries of the invention. The compositions and kits may further comprise additional reagents such as buffers, salts and the like. In some embodiments, the compositions are pharmaceutical compositions optionally comprising one or more pharmaceutical carriers and/or excipients.

Examples

Example 1: Yeast-Based Phage Engineering

[0120] A yeast-based phage engineering platform was developed for capturing and engineering phage genomes with unprecedented speed and ease. FIG. 1 depicts one embodiment of the phage engineering method. A phage genome, or all genomic elements, was prepared to be assembled by polymerase chain reaction (PCR) or DNA synthesis, as follows. Each adjacent DNA fragment had homologous overhangs, which were required for gap-repair cloning.

[0121] The majority of the phage genome was cloned without alteration and obtained from its own genome by PCR. Purified phage genome was used as PCR template. Phage DNA may also be obtained by simply boiling phage lysates as a 10 fold dilution in TE buffer. A single plaque of the phage of interest (e.g., T7, SP-6, K1-5) was picked from a plate and resuspended in 3 mL lysogeny broth (LB) broth containing about 10.sup.7 receptor bacteria (the exact strain may vary from phage to phage), and the resulting culture was incubated at 37.degree. C. with shaking until lysis was visible. The lysate was sterilized by the addition of 200 .mu.L of chloroform, with vigorous shaking, followed by a 30-minute incubation period at room temperature. Cellular debris and chloroform were removed by centrifugation and the sterile lysate was transferred to a clean tube. The sterile lysate was then titered for concentration on an appropriate receptor strain.

[0122] A 50 mL phage lysate was then started from the stock lysate using the same receptor bacterial strain at the same concentration and a multiplicity of infection of 0.01. The lysate was incubated at 37.degree. C. until complete lysis and was processed as the stock lysate. The lysate was also filtered through a 0.22 .mu.m filter to eliminate as much particulate contaminant as possible. DNaseI and RNaseA were then added to the lysate, incubated 2-3 hours at 37.degree. C., and then chilled to 4.degree. C. To precipitate DNA, 10 m L of an ice-cold solution of 30% PEG6000, 3M NaCl was added to the lysate, and the mixture was incubated at 4.degree. C. for at least 2 hours or overnight. Phage particles were spun down at 10000.times.g for 30 minutes, the supernatant discarded, and the pellets drained of all remaining liquids. The pellet was then resuspended in 500 .mu.L-1 mL of buffer SM (100 mM NaCl, 8 mM MgSO.sub.4.7H.sub.2O, 50 mM Tris-CLAIM (1M, pH 7), 0.002% (w/v) gelatin (2% w/v)) and stored at 4.degree. C. To extract DNA, 200 .mu.L of the concentrated lysate was processed with the ZR Viral DNA Kit.TM. using Zymo-Spin.TM. IC-XL Columns (Zymo Research Corporation).

[0123] PCR primers were chosen along the phage genome using the following parameters: (1) span all the genes necessary for a viable phage, and (2) provide at least 30 base pairs (bp) of homology between each PCR product to be assembled. The primers flanking the phage genome contained at least 30 bp homology to the YAC fragment, described below. Examples of primers used to reconstruct several phages are presented in Table 1. The phage genome PCR fragments were amplified using either KAPA HiFi.TM. or KAPA2G.TM. Robust polymerase (Kapa Biosystems). Vector maps and sequences of Enterobacteria phage T7 (SEQ ID NO:1), Enterobacteria phage SP-6 (SEQ ID NO:2), Enterobacteria phage K1-5 (SEQ ID NO:3), and pRS415 (SEQ ID NO:34) are shown in FIGS. 7-10, respectively.

TABLE-US-00001 TABLE 1 PCR Primers for Phage Reconstructions SEQ ID Primer Sequence (5'.fwdarw.3') NO: Description T7-3 GTTTTTGAACACACATGAACAAGGAA 4 T7 GTACAGGTCTCACAGTGTACGGACCTA AAGTTCC P5T227 TTACGCGAACGCGAAGTCCGACTCTAA 5 T7 GAT P5T228 CCAGTTGCACGAGTCTCAATTGGACAA 6 T7 AAT P5T231 TCAGTGGCAAATCGCCCAATTAGGACC 7 T7 CAT P5T84 CCGAAGGTAAGATGGGTCCTAATT 8 T7 P5T235 TTAAATACCGGAACTTCTCCGTAAGTA 9 T7 GTT P5T236 GTTCAACACTGTATACATCTTGTCAGA 10 T7 TGA T7-4 GAAATGTGCGCGGAACCCCTATTTGTT 11 T7 TATAGGGACACAGAGAGACACTCAAG GTAACAC 3'T3- CAGTATGATAGTACATCTCTATGTGTC 12 for capturing pRS415-F-4 CCTTGTCTCATGAGCGGATACATATTT T3 genome GAATGT 5'T3- GGGGGTACTTTGGGTTCTTGAACTATG 13 for capturing pRS415-R-2 AGACCTTGTTCATGTGTGTTCAAAAAC T3 genome GTTATA 3'T7- GTGTTACCTTGAGTGTCTCTCTGTGTCC for capturing pRS415-F-4 CTTGTCTCATGAGCGGATACATATTTG 14 T7 genome AATGT 5'T7- GGGGGAACTTTAGGTCCGTACACTGTG 15 for capturing pRS415-R-2 AGACCTTGTTCATGTGTGTTCAAAAAC T7 genome GTTATA LUZ19_AS TCCTGTCGGGTGGTGGTGCGGGAGTGG 16 for capturing B_Y2_Fw CTATGTCTCATGAGCGGATACATATTT LUZ19 GAATGT genome LUZ19_AS GGAAGGGTGGGCTGATCAGAGTCGGG 17 for capturing B_Y2_Rev AGGGCCTTGTTCATGTGTGTTCAAAAA LUZ19 CGTTATA genome pRS415-F-4 TGTCTCATGAGCGGATACATATTTGAA 18 for capturing TGT T3/T7/K11 PCR products pRS415-R-2 CCTTGTTCATGTGTGTTCAAAAACGTT 19 for capturing ATA T3/T7/K11 PCR products P5T255 CCTGTACTTCCTTGTTCATGTGTGTTCA 20 for capturing AA SP6/K1-5 PCR products P5T256 ATAAACAAATAGGGGTTCCGCGCACA 21 for capturing TTTC SP6/K1-5 PCR products pRS415-R- TATAACGTTTTTGAACACACATGAACA 22 T3 2-T3-1-30-F AGGTCTCATAGTTCAAGAACCCAAAGT ACCCCC T3-9971- ACGGAACCTCCTTCTTGGGTTCTTTGA 23 T3 10000-R CGC T3-9961- CCAGTGGCTGGCGTCAAAGAACCCAA 24 T3 9990-F GAAG T3-19931- GGAAGTCGGTTCATCGCTAAGCACGAT 25 T3 19960-R TGC T3-19921- TGGCGATGATGCAATCGTGCTTAGCGA 26 T3 19950-F TGA T3-29891- GATGCAACGTTCAGCGCAGCACTTTCG 27 T3 29920-R GCA T3-29881- TTGTAGTTGGTGCCGAAAGTGCTGCGC 28 T3 29910-F TGA pRS415-F- ACATTCAAATATGTATCCGCTCATGAG 29 T3 4-T3-38179- ACAAGGGACACATAGAGATGTACTAT 38208-R CATACTG T3-33249- AACAGCGTCGCGGTCATCCACAGCGTT 30 for 33278-R CGC synthesizing T3-7 T3-T7- GCGAACGCTGTGGATGACCGCGACGC 31 for gp17-F-1 TGTTCCGTTTGGTCAACTAAAGACCAT synthesizing GAACCAG T3-7 T3-T7- GTGGACTTAAAGTAGTTCCTTTGATGC 32 for gp17-R-1 TTATTACTCGTTCTCCACCATGATTGCA synthesizing TTAGG T3-7 T7-T3- CCTAATGCAATCATGGTGGAGAACGA 33 for gp17-F-1 GTAATAAGCATCAAAGGAACTACTTTA synthesizing AGTCCAC T3-7 pRS415-R- TATAACGTTTTTGAACACACATGAACA 35 T7 2-1-30-F AGGTCTCACAGTGTACGGACCTAAAGT TCCCCC 9971- ATTACGCGATGACAGTAGACAACCTTT 36 T7 10000-R CCG 9960-9989- TGCAGCAATACCGGAAAGGTTGTCTAC 37 T7 F TGT 19930- ATATGTCTCCTCATAGATGTGCCTATG 38 T7 19959-R TGG 19920- ACTTGTGACTCCACATAGGCACATCTA 39 T7 19949-F TGA 29890- GAATAACCTGAGGGTCAATACCCTGCT 40 T7 29919-R TGT 29880- GACATGATGGACAAGCAGGGTATTGA 41 T7 29909-F CCCT pRS415-F- ACATTCAAATATGTATCCGCTCATGAG 42 T7 4-39909- ACAAGGGACACAGAGAGACACTCAAG 39938-R GTAACAC 35042- AACAGCATCGCGGTCATCCACGGCGTT 43 for 35071-R CGC synthesizing T7-3 T7-T3- GCGAACGCCGTGGATGACCGCGATGC for gp17-F-2 TGTTCCGTTTGGTCAACTTAAGACCAT 44 synthesizing GAACCAG T7-3 T7-T3- GACTACACGTCTTTCCTTGTGATTTACC 45 for gp17-R-2 AATTACACGTCCTCTACGGCTATTGCT synthesizing GTTGG T7-3 T3-T7- CCAACAGCAATAGCCGTAGAGGACGT 46 for gp17-F-2 GTAATTGGTAAATCACAAGGAAAGAC synthesizing GTGTAGTC T7-3 SP6-1 TTTGAACACACATGAACAAGGAAGTA 47 SP6 CAGGTCTCTCGGCCTCGGCCTCGCCGG GATGTCC SP6-2 CGTCCTGATGTACTGGTAGGTGAGTGC 48 SP6 GGA SP6-3 ATTTGGTGGATGAAGGAAGGGCCGAC 49 SP6 GAAT SP6-4 TTCTCCGTGTAGTTATAGCCTTTCCATA 50 SP6 TA SP6-5 CGGCTTGCTTTTTGAGAAGGCATTCCC 51 SP6 CGA SP6-6 AAGATAATAACTTTGAGGTAATCTTTC 52 SP6 ATC SP6-7 AGATTATGTGTATGGTCGTGATGTCAA 53 SP6 AAT SP6-8 CTGGAACCTTAGCTGCCTCAATGCGAG 54 SP6 GTG SP6-9 CATTTCAAGCAGTAGGTCTGGCACAAA 55 SP6 AGG SP6-10 CTTGTTTGTCAAAGATTTCAGGTACTT 56 SP6 GAC SP6-11 AGGAGGAGTATTTCTTCATAATGAAGA 57 SP6 AGG SP6-12 CCACATACGCATCTGATTAGCTTCAAA 58 SP6 GTT SP6-13 GCAGTTAAAGAGCGCGATGAAGCGAA 59 SP6 GAAG SP6-14 TCAATCCTCCAATAAGTCTACGCTGGC 60 SP6 CTT SP6-15 GCAAATACGATTGGTGTAGGTCAGATG 61 SP6 ACC SP6-16 TAAACCTCCTATTACTATCCAGCCCTC 62 SP6 CCC SP6-17 TTGAGCGGCCTATTACTCACCAGTCTT 63 SP6 CAC SP6-18 GAAATGTGCGCGGAACCCCTATTTGTT 64 SP6 TATTAGCCCACGCCCACACACGCTGTC AAGCGG K1-5*1 GTTTTTGAACACACATGAACAAGGAA 65 K1-5 GTACAGGTCGCCCTCGCCCTCGCCGGG TTGT K1-5*2 GGAGAGTCAGAGGGCTTAAGGTTTACT 66 K1-5 GCT K1-5*3 TGCTATGCTACGCGATGCAGTAGGTGC 67 K1-5 GAA K1-5*4 CAGGGTCACGCATCTCATATGGGTCGA 68 K1-5 AGA K1-5*5 TGGACTTGCTCACCACTGAGGAGTTCC 69 K1-5 TCT K1-5*6 GCTTTGTCAGCCTGCTCAGGGAAGCAA 70 K1-5 GCA K1-5*7 TAACTTCGCTGCTGGTCTGGAGTTCGC 71 K1-5 TCG K1-5*8 TGTGCACTTTGTTCTGCATTCCATGAG 72 K1-5 GCT K1-5*9 TGTGCATCTCTTAATAGAGACCCACCA 73 K1-5 CTC K1-5*10 AAGAAGCTGAGTGGCTATCTGCTGCGC 74 K1-5 AGT K1-5*11 TCTAAGGATGCAGATCAGACTAAGCTA 75 K1-5 GCC K1-5*12 GCCTTAGCTCGTAACTCTTCTTCCGCA 76 K1-5 ATA K1-5*13 TAAAACCGAAGTGTCAGACTTAGGTA 77 K1-5 AAGC

K1-5*14 TATTGCCGCCCCAGCTTACATTCTGTTT 78 K1-5 AA K1-5*15 TTGACGGGTTTTATCCAGAAGGATACT 79 K1-5 TCA K1-5*16 GCTATCTCCTATTACTTTCCAACCCTCC 80 K1-5 CT K1-5*17 TTGAGCGGCCTATTACTAGCCAATCTT 81 K1-5 CAT K1-5*18 GAAATGTGCGCGGAACCCCTATTTGTT 82 K1-5 TATTAGCCCACGCCCTCACACCCTGTC AATCCC pRS415-R- TATAACGTTTTTGAACACACATGAACA 83 K11 2-K11-1-30- AGGTCTCACAGTTTACACTTTTGGTTA F TCCCCC K11-9971- ATTAGAAGTCATCGTCTTCTTCGGCTT 84 K11 10000-R CGC K11-9900- AGCGGACGAATCTCGCAGCCGTAAAC 85 K11 9929-F CTCA K11-19961- TCATCACCTTCGAGGGCCTTAAGGGCT 86 K11 19990-R GAC K11-19950- ATTGCCGCATGGTCAGCCCTTAAGGCC 87 K11 19979-F CTC K11-29950- CATCGTGTCCTTGAACACATCGTACCC 88 K11 29979-R ATC 29880- CGGGGACGCTGCTGAGGCTCAGATTCA 89 K11 29909-F GAA pRS415-F- ACATTCAAATATGTATCCGCTCATGAG 90 K11 4-K11- ACAAGGGACACAGAGACATCAACATA 41152- TAGTGTC 41181-R

[0124] A yeast artificial chromosome (YAC) was also prepared, referred to here as the YAC fragment. Primer PST255 (CCTGTACTTCCTTGTTCATGTGTGTTCAAA; SEQ ID NO:20) and primer PST256 (ATAAACAAATAGGGGTTCCGCGCACATTTC; SEQ ID NO:21) were used to PCR amplify a fragment from pRS415 (FIG. 10, SEQ ID NO:34), which contained a yeast centromeric origin, autonomous replication sequence and LEU2 marker.

[0125] All DNA fragments were mixed together with an excess of 100 ng-300 ng of the YAC fragments, and then transformed into yeast BY4741 using a lithium acetate transformation method (e.g., Finlayson, S. D. et al. Biotechnology Techniques, 5(1):13-18, 1991). After a 45 minute heat shock, the cells were spun down, resuspended in Synthetic Complete medium and immediately plated onto SC-leu plates. The plates were then incubated for 2-3 days at 30.degree. C. until colonies appeared. Competent yeast may be prepared in advance in large batches, aliquots placed into freezing medium (DMSO 10%, Glycerol 5%), and stored at -80.degree. C. The colonies were streaked again onto fresh SC-leu plates at 30.degree. C. Colonies were then picked, 3 ml liquid SC leu cultures were inoculated with the colonies at 30.degree. C. for 1 to 2 days until saturated.

[0126] The cultures were spun down and the supernatant discarded. DNA was obtained using the YeaStar.TM. Genomic DNA Kit (Zymo Research) according to the manufacturer's instruction, with the exception that more cells than recommended were loaded into the system, and the Zymolyase.RTM. incubation period was increased from 2 hours to overnight until cell wall digestion was clearly visible through clearing of the mixture. The final elution volume was 50 .mu.L, which resulted in about 5 .mu.g of DNA total.

[0127] Competent cells with a transformation efficiency of about 10.sup.9 as measured from pUC19 transformation will produce about 1 pfu/ng of DNA when using purified T7 DNA. The yeast genome is about 12 mb and the phage::YAC constructs are about 50 kb, thus it can be assumed that 1/250 of the total DNA extracted from yeast is actual phage::YAC DNA. Thus, 5 .mu.l (500 ng to 1 .mu.g) of total DNA from the yeast clones was than transformed into DH10B electro-competent bacteria for phage expression, and the cells were immediately resuspended in LB. If the phage was able to grow on DH10B (such as T7), the resuspended transformed cells were immediately mixed with 3 mL of top agar and plated onto an LB plate. This yielded between 1 and 50 pfu. If the phage was not able to grow on DH10B (such as SP6 or K1-5), the transformed cells were incubated at 37.degree. C. without shaking for 3 hours. The cells were then killed by chloroform addition, and any debris was spun down. The supernatant was then recovered, mixed with 100 .mu.L of an appropriate overnight phage recipient culture, and finally plated onto LB plates by way of 3 mL top agar. The 3 hours incubation permitted the successfully transformed cells to go through one burst liberating phages in the supernatant. This yielded hundreds of plaques because the phage amplified in each DH10B that has received a viable phage genome. The bacterial plaques were picked and sequenced, and then the synthetic phages were recovered.

[0128] To confirm that purified phage DNA from various Gram-negative phages could be transformed into bacterial hosts to generate functional phages, the "E. cloni" 10G strain (10G) was used as a one-time phage propagation host. All phage genomes used in this study were extracted, and up to 4 .mu.g of each genome was electroporated into 10G directly. After incubation, chloroform was added to kill the cells and release phages. Next, supernatant was mixed with overnight culture of natural host bacteria and soft agar, poured onto agar plate, and incubated for 4-18 h to make phage plaques. Except for Pseudomonas phage LUZ19, all phage plaques including Salmonella and Klebsiella phages were found (Table 2), indicating that 10G can be used as an initial host for phage recovering from YAC::phage construct.

TABLE-US-00002 TABLE 2 One-time Phage Propagation Assay Propagation Plaque formation Phage in E. cloni 10G on E. cloni 10G Host bacteria T7 Yes Yes E. coli (ex. BL21) T3 Yes Yes E. coli (ex. BL21) K1-5 Yes No IJ1668 (E. coli K-12 hybrid; K1 capsule) SP6 Yes No IJ612 (S. typhimurium LT2) LUZ19 No No P. aeruginosa PAO1 gh-1 Yes No P. putida C1S K11 Yes No IJ284 (Klebsiella sp. 390)

[0129] To validate the yeast-based phage engineering platform and to determine whether phage genomes assembled in yeast remain viable, several wild-type phages (e.g., T3, T4, T5, T7, K1F, K11, and SP6 were captured and recovered. FIG. 2 shows the results of the validation for T7 phage. PCR was used to linearize the YAC pRS415 while also adding overhangs homologous to the ends of the phage genome. To prevent the appearance of false-positive colonies, PCR-amplified YAC was excised and purified from an agarose gel after electrophoresis. Both the amplified and purified YAC and phage genome were co-transformed into yeast. Transformants were suspended in lysis buffer and disrupted by beads beating. The supernatant containing the YAC::phage constructs was used directly for transfection into E. coli DH10B cells, which are suitable for maintaining large DNA constructs (Durfee et al., Journal of Bacteriology, 190, 2597 (2008)). Among the 16 yeast transformants selected, all were positive by PCR and produced phage, giving an efficiency of 100%.

[0130] Next, wild-type T3 and T7 phages were captured and recovered from each of the four .about.10 kbp PCR products plus the YAC. In this case, PCR was used to linearize the YAC but did not add overhangs. Instead, a homologous region was added to the end of the PCR-amplified YAC, specifically to the 5' and 3' terminal of the first and fourth 10 kbp fragments, respectively, to avoid excision and purification of all 10 kbp fragments from the gel. Among the 16 yeast transformants selected, 15 were positive by PCR and all produced phage, giving an efficiency of 94%.

[0131] Thus, the yeast-based phage engineering platform of the invention can be used to capture and recover phages efficiently and can be used to engineer desired phages from PCR products in one step.

Materials and Methods

Yeast, Bacteria, and Phages.

[0132] Saccharomyces cerevisiae BY4741 (MATa his3.DELTA.1 leu2.DELTA.0 met15.DELTA.0 ura3.DELTA.0) was obtained from Thermo Scientific. Escherichia coli BL21 [B, F.sup.- ompT hsdS.sub.B (r.sub.B.sup.- m.sub.B.sup.-) gal dcm], DH5.alpha. [K-12, F.sup.- .lamda..sup.- .PHI.80d lacZ.DELTA.M15 .DELTA.(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (r.sub.K.sup.- m.sub.K.sup.+)phoA supE44 thi-1 gyrA96 relA1], DH10B [K-12, F.sup.- .lamda..sup.- mcrA .DELTA.(mrr-hsdRMS-mcrBC) .PHI.80d lacZ.DELTA.M15 .DELTA.lacX74 deoR recA1 araD139 .DELTA.(ara leu)7697 galU galK rpsL endA1 nupG], BW25113 [K-12, F.sup.- .lamda..sup.- .DELTA.(araD-araB)567 .DELTA.lacZ4787(::rrnB-3) rph-1 .DELTA.(rhaD-rhaB)568 hsdR514], and MG1655 (K-12, F.sup.- .lamda..sup.- ilvG.sup.- rfb-50 rph-1) were laboratory stocks. Phage T7 (ATCC BAA-1025-B2) and T3 (ATCC 110303-B3) were laboratory stocks.

Determination of Plaque-Forming Unit (pfu).

[0133] Serial dilutions of phage performed in 0.95% saline were added to 300 .mu.l overnight bacterial culture in 3.5 ml soft agar, and poured the mixture onto LB plate. After 3 h incubation at 37.degree. C., plaques were counted.

Preparation of Yeast Competent Cells.

[0134] S. cerevisiae BY4741 was grown in 5 ml YPAD medium (e.g., yeast extract, peptone, glucose, adenine hemisulphate, distilled water, cacto-agar) at 30.degree. C. 300 rpm for 24 hours. Overnight culture was added into 50 ml YPAD medium and incubated at 30.degree. C. 300 rpm for 4 hours. Cells were harvested by centrifugation at 3000 g.

Example 2: Model Phages with Tunable Host Ranges

[0135] To create engineered model phages with tunable host ranges, T7 and T3 phages were selected. They are obligate lytic phages and were originally isolated as a member of the seven "Type" phages that grow on E. coli B (Demerec, M. et al. Genetics 30, 119 (1945)). They have almost same size of linear genome (T7, 39937 bp; T3, 38208 bp), similar gene organization, same life cycle, and high homology across the genomes (Dunn, J. J., et al. Journal of Molecular Biology 166, 477 (1983); Pajunen, M. I., et al. Journal of Molecular Biology 319, 1115 (2002)). Their primary host determinant, tail fiber, consists of single gene product gp17, and importantly, recognizes different host receptors and shows different host ranges (Molineux, I. J., in The Bacteriophages, R. Calendar, Ed. (Oxford Univ. Press, New York, 2006) pp. 277-301). Because there is little information about the difference of host specificities between T7 and T3, their host range was first examined. Based on a previous report that T3 fails to adsorb to many common laboratory E. coli K-12 strains (Molineux, I. J., 2006), plaque formation assays were performed with four K-12 strains. As shown in FIG. 3, T7 can grow efficiently on all strains, while T3 showed poor propagation on BW25113 and MG1655 strains. To assess whether this phenomenon resulted from less adsorption efficiency or post-adsorptive problems, adsorption assays were performed (FIG. 4). Compared with BL21 reference strain, the level of adsorption abilities of T3 on BW25113 and MGI655 were .about.10.sup.4% less efficient, which is consistent with the result of plaque formation assay (FIG. 3). These results indicate that T7 and T3 have different host range, and BW25113 and MG1655 are useful for validating synthetic T7 and T3 phages with engineered tail fiber.

[0136] The tail fibers of T7 and T3 are encoded by gene 17, and the gene product gp17 can be split in two domains. The N-terminal 149 residues are necessary for the tail fiber to bind to the rest of the capsid, while the remaining C-terminal region recognizes the host receptors at bacterial surfaces (Steven, A. C., et al. Journal of Molecular Biology 200, 351 (1988)). Between T7 and T3 phages, N-terminal regions have 99% identity while C-termini have 83% in protein level. Similar but clearly different host range among these phages can be explained by differences in the distal portion of the tail fiber gene. This indicated that engineering the C-terminal domain of gp17 with tail fiber modules could produce synthetic phages with altered host ranges. To create synthetic T7 phage with T3 tail fiber (T7-3) and T3 phage with T7 tail fiber (T3-7) in one-step using the yeast-based phage engineering platform, six PCR fragments derived from each phages and PCR-amplified excised YAC were prepared (FIGS. 5A and 5B shows synthetic T3). All fragments were co-transformed into yeast, and transformants were suspended in lysis buffer and disrupted by beads beating. The supernatant containing the engineered phage genome was used directly for transfection into E. coli DH10B. By using same method, three synthetic phages (T7-3, T3-7, T7) with wild-type tail fiber (T7-wt) and T3 phage with wild-type tail fiber (T3-wt) were also engineered (FIGS. 5A and 5B).

[0137] To create synthetic T7 phages with T3 tail fibers in one-step using the yeast platform system as provided herein, nine PCR fragments derived from each phages plus PCRed-excised YAC were prepared (FIG. 5C for synthetic T7). Fragments were co-transformed into yeast, and transformants were enzymatically disrupted for YAC::phage extraction. The engineered phage genome was used for transformation into bacterial strain 10G. Six synthetic phages (T7 phage with wild-type tail fiber (T7.sub.WT), T7 phage with C-terminal T3 tail fiber (T7.sub.T3gp17-partial), T7 phage with entire T3 tail fiber (T7.sub.T3gp17), T3 phage with wild-type tail fiber (T3.sub.WT), T3 phage with C-terminal T7 tail fiber (T3.sub.T7gp17-partial), and T3 phage with entire T7 tail fiber (T3.sub.T7gp17)) were engineered.

[0138] To examine their host specificities, plaque formation assays were performed with K-12 strains described above (FIGS. 6A and 6B). T3-7 phage (e.g., T3.sub.T7gp17-partial and T3.sub.T7gp17) grew on BW25113 and MG1655 strains with efficiency similar to that of T7, while T3 and T3-wt propagated poorly. By contrast, T7-3 (e.g., T3.sub.T7gp17-partial and T3.sub.T7gp17) did not grow on BW25113 and MG1655. Plaque formation assays were also performed with two E. coli libraries, ECOR group and DECA set, to confirm details of host range of T7, T3, and synthetic phages. As shown in FIG. 6C, T7 phages, T3 phages, and synthetic phages infected ECOR4 and ECOR13. While T3 infected ECOR16, T7 did not, and while T7.sub.T3gp17-partial and T7.sub.T3gp17 infected ECOR16, T3.sub.T7gp17-partial and T3.sub.T7gp17 did not. These results clearly indicate that the C-terminal region of gp17 is the host range determinant, and the synthetic engineered phages with different tail fibers acquired tail-fiber-dependent host specificities.

[0139] Next, phages were engineered with fully synthesized tail fiber. A codon-optimized gene encoding tail fiber was synthesized from the T7-like Enterobacteria phage 13a. T7 phage with 13a phage tail fiber was engineered and its functionalities confirmed (FIG. 6B, lanes 5 and 6). T7.sub.13agp17-partial infected BW25113 and MG1655 strains but T7.sub.13agp17 did not. This result indicates that not only C-terminal part but also N-terminal one are responsible for host specificity.

Example 3: Model Phages with Tunable Host Ranges Between Species

[0140] To demonstrate that phages could overcome the species barrier, Escherichia coli (E. coli) phage T3 and Yersinia phage R hybrids were engineered. T3 and R phages have similar gp17 sequences, with the exception of 3 residues; however, while R phage can infect Yersinia strains IP2666 and YPIII, T3 cannot. R phage (Rgp17) was engineered by PCR using T3 gp17 and primers having desired mutations. Synthetic T3 with R tail fiber (T3.sub.Rgp17) was functional and infected Yersinia IP2666 and YPIII as well as E. coli BL21.

[0141] Phages were also engineered with less similarity. E. coli phage T7 and Klebsiella phage K11 were selected because their host ranges are different and do not overlap. K11 is a T7-like phage and relative to T7 has a similarly sized linear genome, similar gene organization, and similar life cycle. The genome identity between the two strains, however, is low. At the genomic level, T7 and K11 share 59% identity, while T7 and T3 share 72% identify. In the tail fiber gp17, T7 and K11 share only 23% identity, while T7 and T3 share 86%. In addition, K11 has a 322 residue longer tail fiber compared with T7. To create T7 phage with K11 tail fiber and K11 phage with T7 tail fiber, the same strategy was used as described above.

[0142] In this experiment, neither synthetic K11 phages with T7 gp17 tail fibers nor synthetic T7 phages with K11 gp17 tail fibers were recovered. Further, hybrid phages with various lengths of gp17 were designed but not recovered. Because the K11 phage is propagated in the 10G strain, as described above, it is unlikely that synthetic K11 phage with T7 gp17 tail fibers can adsorb E. coli but cannot produce progeny phages, which indicates that, at least in K11 phage, swapping only the tail fiber is not sufficient to produce a functional synthetic phage.

[0143] The tail of T7 phage is formed by a tubular structure (gp11 and gp12) surrounded by six tail fibers (gp17), and the interface between gp11 and gp12 interacts with six gp17 trimers to generate the complete tail (FIG. 11A). In addition, phage K11 spikes contained in the tail have depolymerase activity to degrade host Klebsiella capsular polysaccharide for infection. In view of the foregoing, all the tail components (gp11, gp12, and gp17) between T7 and K11 were replaced (FIG. 11B). Surprisingly, both synthetic phages, T7 with K11 tail (T7.sub.K11gp11-12-17) and K11 with T7 tail (K11.sub.T7gp11-12-17), were functional and showed tail-dependent host range. T7.sub.K11gp11-12-17 infected Klebsiella, and K11.sub.T7gp11-12-17 infected E. coli (FIG. 6B, lanes 7 and 14).

[0144] The plaque assay, shown in FIG. 12A, demonstrated that synthetic T3 with R tail fiber (T3.sub.Rgp17) is capable of infecting Escherichia coli strain BL21 and Yersinia strains IP2666 and YPIII. The plaque assay also demonstrated that synthetic T7 phages with K11 tail fibers (T7.sub.K11gp11-12-17) are capable of infecting Klebsiella and that synthetic K11 phages with T7 tail fibers (K11.sub.T7gp11-12-17) are capable of infecting BL21. The percent adsorption efficiencies of T7.sub.WT, K11.sub.WT, T7.sub.K11gp11-12-17, and K11.sub.T7gp11-12-17 are shown in FIG. 12B.

[0145] The Example herein demonstrates an efficient and simple yeast-based platform for phage engineering and that phage host range can be altered with synthetic biology techniques.

[0146] This design may be adapted to be compatible with other phages and viruses. Synthetic biology approaches, described herein, address an important problem for phage-based therapeutics and diagnostics relating to limited phage host range. The methods of the present disclosure may also be used for other applications in biology, veterinary sciences, food sciences and medicine.

Materials and Methods

Strains, Vector, and Primers.

[0147] Phages T7 (ATCC BAA-1025-B2) and T3 (ATCC 110303-B3) were laboratory stocks. Phages K1-5 and K11 were provided by University of Texas at Austin. Phage LUZ19 were provided KU Leuven. Phage gh-1 (ATCC 12633-B1) was obtained from ATCC. Synthetic phages are listed in Table 3. Saccharomyces cerevisiae BY4741 (MATa his3D1 leu2D0 met15D0 ura3D0) was obtained from Thermo Scientific. Escherichia coli BL21 [B, F ompT hsdS.sub.B (r.sub.B.sup.- m.sub.B.sup.-) gal dcm], DH5.alpha. [K-12, F.sup.- l.sup.- F80d lacZDM15 D(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (r.sub.K.sup.- m.sub.K.sup.+)phoA supE44 thi-1 gyrA96 relA1], BW25113 [K-12, F.sup.- l.sup.- D(araD-araB)567 DlacZ4787(::rrnB-3) rph-1 D(rhaD-rhaB)568 hsdR514], and MG1655 (K-12, F.sup.- l.sup.- ilvG.sup.- rfb-50 rph-1) were laboratory stocks. E. cloni 10G [K-12, F D(ara leu)7697 araD139 DlacX74 galU galK F80d lacZDM15 recA1 endA1 nupG1 rpsL (Str.sup.R) D(mrr-hsdRMS-mcrBC) tonA] were obtained from Lucigen. 10G is a DH10B derivative and is suitable for maintaining large DNA constructs. Bacterial strains IJ284 Klebsiella sp. 390 (03:K11), IJ1668 K-12 hybrid; K1 capsule, and LT612 Salmonella typhimurium LT2 were provided by University of Texas at Austin. Yersinia pseudotuberculosis IP2666 and YPIII were provided by Tufts University. E. coli libraries, ECOR group and DECA set, were obtained from Michigan State University. Pseudomonas putida (ATCC 23287) was obtained from ATCC. pRS415 yeast centromere vector with LEU2 marker (ATCC 87520) was laboratory stock. Primers are listed in Table 1.

TABLE-US-00003 TABLE 3 Synthetic phages Phage Genotype Description T7.sub.WT wild-type synthesized from PCR fragments T7.sub.T3gp17 T7.sub.WT.DELTA.gene 17 carrying T3 T7 with T3 tail fiber gene 17 T7.sub.T3gp17-partial T7.sub.WT.DELTA.gene 17(1-450) carrying T7 with T7-T3 T3 gene 17(451-1677) hybrid tail fiber T7.sub.T3agp17 T7.sub.WT.DELTA.gene 17 carrying 13a gene T7 with 13a tail 17 fiber T7.sub.K11gp11-12-17 T7.sub.WT.DELTA.genes 11-12 17 carrying T7 with K11 tail K11 genes 11-12 17 T3.sub.WT wild-type synthesized from PCR fragments T3.sub.T7gp17 T3.sub.WT.DELTA.gene 17 carrying T7 T3 with T7 tail fiber gene 17 T3.sub.T7gp17-partial T3.sub.WT.DELTA.gene 17(1-450) carrying T3 with T3-T7 T7 gene 17(451-1662) hybrid tail fiber T3.sub.Rgp17 T3.sub.WT.DELTA.gene 17 carrying R gene T3 with R tail fiber 17 K11.sub.WT wild-type synthesized from PCR fragments K11.sub.T7gp11-12-17 K11.sub.WT.DELTA.genes 11-12 17 carrying K11 with T7 tail T7 genes 11-12 17

Culture Conditions.

[0148] Unless otherwise specified, BY4741 and bacterial strains were cultured in YPD medium [1% Bacto Yeast Extract (BD), 2% Bacto Peptone (BD), 2% dextrose (VWR)] at 30.degree. C. and in LB medium (BD) at 37.degree. C., respectively.

Preparation of Linearized pRS415.

[0149] pRS415 was linearized by using PCR amplification with specific primer sets (Table 1) and KAPA HiFi DNA Polymerase (Kapa Biosystems). For genome capturing, 5' and 3' terminal 30-40 bp of phage homologous sequence were added to the pRS415. Linearized pRS415 was purified from an agarose gel following electrophoresis with QIAquick Gel Extraction Kit (Qiagen).

Preparation of Phage Genome.

[0150] After preparation of 200 ml phage lysate (10.sup.9-10.sup.12 cfu/ml), 200 .mu.l chloroform (Sigma) was added to kill the host bacteria and release phages. Lysate was centrifuged at 8,000 g for 5 min and then filtrated with 0.2 .mu.m filter (VWR) to remove cell debris. 216 .mu.l of buffer L1 [20 mg/ml RNase A (Sigma), 6 mg/ml DNase I (NEB), 0.2 mg/ml BSA (NEB), 10 mM EDTA (Teknova), 100 mM Tris-HCl (VWR), 300 mM NaCl (VWR), pH 7.5] was added and incubated at 37.degree. C. for 1 h with gentle shaking. Then 30 ml of ice cold buffer L2 [30% polyethylene glycol (PEG) 6000 (Sigma), 3 M NaCl] was added and stored overnight in 4.degree. C. The sample was centrifuged at 10,000 g for 30 min at 4.degree. C. The phage pellet was suspended in 9 ml buffer L3 (100 mM Tris-HCl, 100 mM NaCl, 25 mM EDTA, pH7.5). Then, 9 ml buffer L4 [4% SDS (VWR)] was added and incubated at 70.degree. C. for 20 min. After cooling down on ice, 9 ml buffer L5 [2.55 M potassium acetate, pH4.8 (Teknova)] was added, and the sample was centrifuged at 10,000 g for 30 min at 4.degree. C. Phage genome in the supernatant was purified by using Qiagen-tip 100 (Qiagen) according to the manufacturer's instructions.

Preparation of PCR Products for Assembling Phage Genome.

[0151] All PCR products were prepared with specific primer sets (Table 1) and KAPA HiFi DNA Polymerase. To avoid excision and purification of all PCR products from an agarose gel, homologous region of the end of linearized pRS415 was added to 5' and 3' terminus of first and last PCR products, respectively.

Preparation of Yeast Competent Cells.

[0152] S. cerevisiae BY4741 was grown in 5 ml YPD medium at 30.degree. C. for 24 h. Overnight culture was added into 50 ml YPD medium, and incubated at 30.degree. C. for 4 h. Cells were harvested by centrifugation at 3,000 g and washed with 25 ml water and then with 1 ml of 100 mM lithium acetate (LiAc) (Alfa Aesar), and suspended in 400 .mu.l of 100 mM LiAc. Fifty microliter was used for a transformation.

Yeast Transformation.

[0153] All DNA samples and a linearized pRS415 were collected in a tube (0.5-4.0 .mu.g each DNA sample and 100 ng linearized pRS415 in 50 .mu.l water), and mixed with transformation mixture [50 .mu.l yeast competent cell, 240 .mu.l 50% PEG3350 (Sigma), 36 .mu.l 1 M LiAc, 25 .mu.l 2 mg/ml salmon sperm DNA (Sigma)]. The mixture was incubated at 30.degree. C. for 30 min, then at 42.degree. C. for 20 min, centrifuged at 8,000 g for 15 sec, and suspended in 200 .mu.l water. Transformants were selected on complete synthetic defined medium without leucine (SD-Leu) [0.67% YNB+Nitrogen (Sunrise Science Products), 0.069% CSM-Leu (Sunrise Science Products), 2% dextrose] agar plates at 30.degree. C. for 3 days.

Extraction of Captured Phage Genome.

[0154] Individual yeast transformants were picked into 2 ml SD-Leu liquid medium and incubated at 30.degree. C. for 24 h. DNA was extracted from these cells using the YeaStar Genomic DNA Kit (Zymo Research) or Yeast Genomic DNA Purification Kit (Amresco) according to the manufacturer's instructions.

Reviving of Phage.

[0155] Except for phage LUZ19, the 10G strain was used as a host bacterium for initial propagation of phage. To revive T7 and T3 phages, 5 .mu.l of extracted DNA were electroporated into 100 .mu.l cells in a 2 mm gap electroporation cuvette (Molecular BioProducts) at 2,500 V, 25 .mu.F, 200.OMEGA. using a Gene Pulser Xcell (Bio-Rad). Cells were mixed with 3 ml LB soft agar (LB contains 0.6% agarose) warmed at 55.degree. C., poured onto LB plate, and incubated for 4 h at 37.degree. C. To revive SP6, K1-5, and K11, after electroporation, cells were incubated at 37.degree. C. for 1 h in 1 ml LB medium. Then, some drops of chloroform were added to kill the cells and release phages. After centrifugation at 12,000 g for 1 min, supernatant was mixed with 100 .mu.l overnight culture of natural host bacteria, i.e. LT612 S. typhimurium LT2 for SP6, IJ1668 K-12 hybrid; K1 capsule for K1-5, and J284 Klebsiella sp. 390 (03:K11) for K11, and 3 ml LB soft agar, poured onto LB plate, and incubated for 4-18 h at 37.degree. C. For LUZ19, P. aeruginosa PAO1 was used as a host bacterium. All extracted DNA from 2 ml overnight culture was electroporated into competent PAO1 cells with same condition as described above. After electroporation, cells were incubated at 37.degree. C. for 2.5 h in 1 ml LB medium. Cells were mixed with 3 ml LB soft agar, poured onto LB plate, and incubated for 18 h at 37.degree. C.

One-Time Phage Propagation Assay.

[0156] To check the ability of the 10G strain as a one-time phage propagation plant, 0.5-4.0 .mu.g of purified phage genome was electroporated into the cell. The condition of electroporation and the following procedures were exactly same as described in "Reviving of phage".

Adsorption Assay.

[0157] Each 100 .mu.l of 2.times.10.sup.8 cfu/ml E. coli and 1.times.10.sup.8 pfu/ml phage, were miced and incubated at RT for 10 min. Then, 700 .mu.l of 0.95% saline and some drops of chloroform was added to kill the cells and prevent the production of progeny phages. After centrifugation at 11,000 g for 1 min, supernatant was serially diluted and mixed with 100 .mu.l of E. coli BL21 overnight culture and 3 ml LB soft agar, and poured the mixture onto LB plate. After 3 h incubation at 37.degree. C., phage plaques were counted, and adsorption efficiency was calculated. Adsorption efficiency (%)=[1-(pfu of unadsorbed phage/original pfu in the BL21 and phage mixture)].times.100

Infection Assay.

[0158] Larvae of the Greater Wax Moth (Galleria mellonella) were purchased in their final larval instar from Vanderhorst Wholesale, Inc. (St. Marys, Ohio, USA). Healthy larvae of around 150-250 mg were sorted from small, darkly colored, or inactive larvae upon receipt and allowed to acclimate at RT in the dark for at least 24 h prior to experiments. For infection assays, an overnight culture of K. pneumoniae was diluted 1:100 into fresh LB and grown to late-log phase at 37.degree. C. for 3 h. Bacteria were washed twice and resuspended in an equal volume of PBS, then further diluted in PBS to yield a final inoculum of approximately 10.sup.6 CFU/larva. A KDS100 syringe pump (KD Scientific) was used to inject 10 .mu.l of PBS or the bacterial suspension behind the last left proleg of each randomly chosen larva. Within 1 h of the first injection, a second injection of 10 .mu.l of sterile LB broth or endotoxin-purified phage lysate was administered behind the last right proleg and larvae were incubated at 37.degree. C. in groups of 5 per petri dish. Survival scoring was performed every 12 h for up to 72 h, with mortality confirmed by lack of response to touch. Data were pooled from three experiments each with 10 larvae per treatment group (n=30) and Kaplan-Meier curves were generated and analyzed by log-rank test using GraphPad Prism version 6.0 (GraphPad Software, San Diego, Calif., USA).

TABLE-US-00004 Bacteriophage Genome Sequences SEQ ID NO: 1-Enterobacteria phage T7 TCTCACAGTGTACGGACCTAAAGTTCCCCCATAGGGGGTACCTAAAGCCCAGCCAATCACCTAAAGTCAACCT- TCGGTTGACCTTGAGGGTTCCCTAAGGGTTGGGGATG ACCCTTGGGTTTGTCTTTGGGTGTTACCTTGAGTGTCTCTCTGTGTCCCTATCTGTTACAGTCTCCTAAAGTA- TCCTCCTAAAGTCACCTCCTAACGTCCATCCTAAAGCCA ACACCTAAAGCCTACACCTAAAGACCCATCAAGTCAACGCCTATCTTAAAGTTTAAACATAAAGACCAGACCT- AAAGACCAGACCTAAAGACACTACATAAAGACCAGA CCTAAAGACGCCTTGTTGTTAGCCATAAAGTGATAACCTTTAATCATTGTCTTTATTAATACAACTCACTATA- AGGAGAGACAACTTAAAGAGACTTAAAAGATTAATTT AAAATTTATCAAAAAGAGTATTGACTTAAAGTCTAACCTATAGGATACTTACAGCCATCGAGAGGGACACGGC- GAATAGCCATCCCAATCGACACCGGGGTCAACCGGA TAAGTAGACAGCCTGATAAGTCGCACGAAAAACAGGTATTGACAACATGAAGTAACATGCAGTAAGATACAAA- TCGCTAGGTAACACTAGCAGCGTCAACCGGGCGCA CAGTGCCTTCTAGGTGACTTAAGCGCACCACGGCACATAAGGTGAAACAAAACGGTTGACAACATGAAGTAAA- CACGGTACGATGTACCACATGAAACGACAGTGAGTC ACCACACTGAAAGGTGATGCGGTCTAACGAAACCTGACCTAAGACGCTCTTTAACAATCTGGTAAATAGCTCT- TGAGTGCATGACTAGCGGATAACTCAAGGGTATCGC AAGGTGCCCTTTATGATATTCACTAATAACTGCACGAGGTAACACAAGATGGCTATGTCTAACATGACTTACA- ACAACGTTTTCGACCACGCTTACGAAATGCTGAAAGA AAACATCCGTTATGATGACATCCGTGACACTGATGACCTGCACGATGCTATTCACATGGCTGCCGATAATGCA- GTTCCGCACTACTACGCTGACATCTTTAGCGTAATGG CAAGTGAGGGCATTGACCTTGAGTTCGAAGACTCTGGTCTGATGCCTGACACCAAGGACGTAATCCGCATCCT- GCAAGCGCGTATCTATGAGCAATTAACGATTGACCTC TGGGAAGACGCAGAAGACTTGCTCAATGAATACTTGGAGGAAGTCGAGGAGTACGAGGAGGATGAAGAGTAAT- GTCTACTACCAACGTGCAATACGGTCTGACCGCTC AAACTGTACTTTTCTATAGCGACATGGTGCGCTGTGGCTTTAACTGGTCACTCGCAATGGCACAGCTCAAAGA- ACTGTACGAAAACAACAAGGCAATAGCTTTAGAATCT GCTGAGTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCACTTTACTTATGAGGGAGTAAT- GTATATGCTTACTATCGGTCTACTCACCGCTCTAGGTC TAGCTGTAGGTGCATCCTTTGGGAAGGCTTTAGGTGTAGCTGTAGGTTCCTACTTTACCGCTTGCATCATCAT- AGGAATCATCAAAGGGGCACTACGCAAATGATGAAGC ACTACGTTATGCCAATCCACACGTCCAACGGGGCAACCGTATGTACACCTGATGGGTTCGCAATGAAACAACG- AATCGAACGCCTTAAGCGTGAACTCCGCATTAACCG CAAGATTAACAAGATAGGTTCCGGCTATGACAGAACGCACTGATGGCTTAAAGAAAGGTTATATGCCCAATGG- CACACTATACGCTGCAAATCGGCGAATAGTGAGAAC TTGGCGAGAGAACAACCTCGAACGCCGCAAGGACAAGAGAGGGCGGCGTGGCATAGACGAAAGGAAAAGGTTA- AAGCCAAGAAACTCGCCGCACTTGAACAGGCACT AGCCAACACACTGAACGCTATCTCATAACGAACATAAAGGACACAATGCAATGAACATTACCGACATCATGAA- CGCTATCGACGCAATCAAAGCACTGCCAATCTGTGA ACTTGACAAGCGTCAAGGTATGCTTATCGACTTACTGGTCGAGATGGTCAACAGCGAGACGTGTGATGGCGAG- CTAACCGAACTAAATCAGGCACTTGAGCATCAAGAT TGGTGGACTACCTTGAAGTGTCTCACGGCTGACGCAGGGTTCAAGATGCTCGGTAATGGTCACTTCTCGGCTG- CTTATAGTCACCCGCTGCTACCTAACAGAGTGATTAA GGTGGGCTTTAAGAAAGAGGATTCAGGCGCAGCCTATACCGCATTCTGCCGCATGTATCAGGGTCGTCCTGGT- ATCCCTAACGTCTACGATGTACAGCGCCACGCTGGAT GCTATACGGTGGTACTTGACGCACTTAAGGATTGCGAGCGTTTCAACAATGATGCCCATTATAAATACGCTGA- GATTGCAAGCGACATCATTGATTGCAATTCGGATGAG CATGATGAGTTAACTGGATGGGATGGTGAGTTTGTTGAAACTTGTAAACTAATCCGCAAGTTCTTTGAGGGCA- TCGCCTCATTCGACATGCATAGCGGGAACATCATGTT CTCAAATGGAGACGTACCATACATCACCGACCCGGTATCATTCTCGCAGAAGAAAGACGGTGGCGCATTCAGC- ATCGACCCTGAGGAACTCATCAAGGAAGTCGAGGAA GTCGCACGACAGAAAGAAATTGACCGCGCTAAGGCCCGTAAAGAACGTCACGAGGGGCGCTTAGAGGCACGCA- GATTCAAACGTCGCAACCGCAAGGCACGTAAAGCA CACAAAGCTAAGCGCGAAAGAATGCTTGCTGCGTGGCGATGGGCTGAACGTCAAGAACGGCGTAACCATGAGG- TAGCTGTAGATGTACTAGGAAGAACCAATAACGCT ATGCTCTGGGTCAACATGTTCTCTGGGGACTTTAAGGCGCTTGAGGAACGAATCGCGCTGCACTGGCGTAATG- CTGACCGGATGGCTATCGCTAATGGTCTTACGCTCAA CATTGATAAGCAACTTGACGCAATGTTAATGGGCTGATAGTCTTATCTTACAGGTCATCTGCGGGTGGCCTGA- ATAGGTACGATTTACTAACTGGAAGAGGCACTAAATG AACACGATTAACATCGCTAAGAACGACTTCTCTGACATCGAACTGGCTGCTATCCCGTTCAACACTCTGGCTG- ACCATTACGGTGAGCGTTTAGCTCGCGAACAGTTGGC CCTTGAGCATGAGTCTTACGAGATGGGTGAAGCACGCTTCCGCAAGATGTTTGAGCGTCAACTTAAAGCTGGT- GAGGTTGCGGATAACGCTGCCGCCAAGCCTCTCATCA CTACCCTACTCCCTAAGATGATTGCACGCATCAACGACTGGTTTGAGGAAGTGAAAGCTAAGCGCGGCAAGCG- CCCGACAGCCTTCCAGTTCCTGCAAGAAATCAAGCC GGAAGCCGTAGCGTACATCACCATTAAGACCACTCTGGCTTGCCTAACCAGTGCTGACAATACAACCGTTCAG- GCTGTAGCAAGCGCAATCGGTCGGGCCATTGAGGAC GAGGCTCGCTTCGGTCGTATCCGTGACCTTGAAGCTAAGCACTTCAAGAAAAACGTTGAGGAACAACTCAACA- AGCGCGTAGGGCACGTCTACAAGAAAGCATTTATGC AAGTTGTCGAGGCTGACATGCTCTCTAAGGGTCTACTCGGTGGCGAGGCGTGGTCTTCGTGGCATAAGGAAGA- CTCTATTCATGTAGGAGTACGCTGCATCGAGATGCTC ATTGAGTCAACCGGAATGGTTAGCTTACACCGCCAAAATGCTGGCGTAGTAGGTCAAGACTCTGAGACTATCG- AACTCGCACCTGAATACGCTGAGGCTATCGCAACCC GTGCAGGTGCGCTGGCTGGCATCTCTCCGATGTTCCAACCTTGCGTAGTTCCTCCTAAGCCGTGGACTGGCAT- TACTGGTGGTGGCTATTGGGCTAACGGTCGTCGTCCTC TGGCGCTGGTGCGTACTCACAGTAAGAAAGCACTGATGCGCTACGAAGACGTTTACATGCCTGAGGTGTACAA- AGCGATTAACATTGCGCAAAACACCGCATGGAAAAT CAACAAGAAAGTCCTAGCGGTCGCCAACGTAATCACCAAGTGGAAGCATTGTCCGGTCGAGGACATCCCTGCG- ATTGAGCGTGAAGAACTCCCGATGAAACCGGAAGAC ATCGACATGAATCCTGAGGCTCTCACCGCGTGGAAACGTGCTGCCGCTGCTGTGTACCGCAAGGACAAGGCTC- GCAAGTCTCGCCGTATCAGCCTTGAGTTCATGCTTGA GCAAGCCAATAAGTTTGCTAACCATAAGGCCATCTGGTTCCCTTACAACATGGACTGGCGCGGTCGTGTTTAC- GCTGTGTCAATGTTCAACCCGCAAGGTAACGATATGA CCAAAGGACTGCTTACGCTGGCGAAAGGTAAACCAATCGGTAAGGAAGGTTACTACTGGCTGAAAATCCACGG- TGCAAACTGTGCGGGTGTCGATAAGGTTCCGTTCCC TGAGCGCATCAAGTTCATTGAGGAAAACCACGAGAACATCATGGCTTGCGCTAAGTCTCCACTGGAGAACACT- TGGTGGGCTGAGCAAGATTCTCCGTTCTGCTTCCTTG CGTTCTGCTTTGAGTACGCTGGGGTACAGCACCACGGCCTGAGCTATAACTGCTCCCTTCCGCTGGCGTTTGA- CGGGTCTTGCTCTGGCATCCAGCACTTCTCCGCGATGC TCCGAGATGAGGTAGGTGGTCGCGCGGTTAACTTGCTTCCTAGTGAAACCGTTCAGGACATCTACGGGATTGT- TGCTAAGAAAGTCAACGAGATTCTACAAGCAGACGC AATCAATGGGACCGATAACGAAGTAGTTACCGTGACCGATGAGAACACTGGTGAAATCTCTGAGAAAGTCAAG- CTGGGCACTAAGGCACTGGCTGGTCAATGGCTGGCT TACGGTGTTACTCGCAGTGTGACTAAGCGTTCAGTCATGACGCTGGCTTACGGGTCCAAAGAGTTCGGCTTCC- GTCAACAAGTGCTGGAAGATACCATTCAGCCAGCTAT TGATTCCGGCAAGGGTCTGATGTTCACTCAGCCGAATCAGGCTGCTGGATACATGGCTAAGCTGATTTGGGAA- TCTGTGAGCGTGACGGTGGTAGCTGCGGTTGAAGCAA TGAACTGGCTTAAGTCTGCTGCTAAGCTGCTGGCTGCTGAGGTCAAAGATAAGAAGACTGGAGAGATTCTTCG- CAAGCGTTGCGCTGTGCATTGGGTAACTCCTGATGGT TTCCCTGTGTGGCAGGAATACAAGAAGCCTATTCAGACGCGCTTGAACCTGATGTTCCTCGGTCAGTTCCGCT- TACAGCCTACCATTAACACCAACAAAGATAGCGAGAT TGATGCACACAAACAGGAGTCTGGTATCGCTCCTAACTTTGTACACAGCCAAGACGGTAGCCACCTTCGTAAG- ACTGTAGTGTGGGCACACGAGAAGTACGGAATCGAA TCTTTTGCACTGATTCACGACTCCTTCGGTACCATTCCGGCTGACGCTGCGAACCTGTTCAAAGCAGTGCGCG- AAACTATGGTTGACACATATGAGTCTTGTGATGTACTG GCTGATTTCTACGACCAGTTCGCTGACCAGTTGCACGAGTCTCAATTGGACAAAATGCCAGCACTTCCGGCTA- AAGGTAACTTGAACCTCCGTGACATCTTAGAGTCGGA CTTCGCGTTCGCGTAACGCCAAATCAATACGACTCACTATAGAGGGACAAACTCAAGGTCATTCGCAAGAGTG- GCCTTTATGATTGACCTTCTTCCGGTTAATACGACTC ACTATAGGAGAACCTTAAGGTTTAACTTTAAGACCCTTAAGTGTTAATTAGAGATTTAAATTAAAGAATTACT- AAGAGAGGACTTTAAGTATGCGTAACTTCGAAAAGAT GACCAAACGTTCTAACCGTAATGCTCGTGACTTCGAGGCAACCAAAGGTCGCAAGTTGAATAAGACTAAGCGT- GACCGCTCTCACAAGCGTAGCTGGGAGGGTCAGTAA GATGGGACGTTTATATAGTGGTAATCTGGCAGCATTCAAGGCAGCAACAAACAAGCTGTTCCAGTTAGACTTA- GCGGTCATTTATGATGACTGGTATGATGCCTATACAA GAAAAGATTGCATACGGTTACGTATTGAGGACAGGAGTGGAAACCTGATTGATACTAGCACCTTCTACCACCA- CGACGAGGACGTTCTGTTCAATATGTGTACTGATTGG TTGAACCATATGTATGACCAGTTGAAGGACTGGAAGTAATACGACTCAGTATAGGGACAATGCTTAAGGTCGC- TCTCTAGGAGTGGCCTTAGTCATTTAACCAATAGGAG ATAAACATTATGATGAACATTAAGACTAACCCGTTTAAAGCCGTGTCTTTCGTAGAGTCTGCCATTAAGAAGG- CTCTGGATAACGCTGGGTATCTTATCGCTGAAATCAA GTACGATGGTGTACGCGGGAACATCTGCGTAGACAATACTGCTAACAGTTACTGGCTCTCTCGTGTATCTAAA- ACGATTCCGGCACTGGAGCACTTAAACGGGTTTGATG TTCGCTGGAAGCGTCTACTGAACGATGACCGTTGCTTCTACAAAGATGGCTTTATGCTTGATGGGGAACTCAT- GGTCAAGGGCGTAGACTTTAACACAGGGTCCGGCCTA CTGCGTACCAAATGGACTGACACGAAGAACCAAGAGTTCCATGAAGAGTTATTCGTTGAACCAATCCGTAAGA- AAGATAAAGTTCCCTTTAAGCTGCACACTGGACACC TTCACATAAAACTGTACGCTATCCTCCCGCTGCACATCGTGGAGTCTGGAGAAGACTGTGATGTCATGACGTT- GCTCATGCAGGAACACGTTAAGAACATGCTGCCTCTG CTACAGGAATACTTCCCTGAAATCGAATGGCAAGCGGCTGAATCTTACGAGGTCTACGATATGGTAGAACTAC- AGCAACTGTACGAGCAGAAGCGAGCAGAAGGCCATG AGGGTCTCATTGTGAAAGACCCGATGTGTATCTATAAGCGCGGTAAGAAATCTGGCTGGTGGAAAATGAAACC- TGAGAACGAAGCTGACGGTATCATTCAGGGTCTGGT ATGGGGTACAAAAGGTCTGGCTAATGAAGGTAAAGTGATTGGTTTTGAGGTGCTTCTTGAGAGTGGTCGTTTA- GTTAACGCCACGAATATCTCTCGCGCCTTAATGGATG AGTTCACTGAGACAGTAAAAGAGGCCACCCTAAGTCAATGGGGATTCTTTAGCCCATACGGTATTGGCGACAA- CGATGCTTGTACTATTAACCCTTACGATGGCTGGGCG TGTCAAATTAGCTACATGGAGGAAACACCTGATGGCTCTTTGCGGCACCCATCGTTCGTAATGTTCCGTGGCA- CCGAGGACAACCCTCAAGAGAAAATGTAATCACACTG GCTCACCTTCGGGTGGGCCTTTCTGCGTTTATAAGGAGACACTTTATGTTTAAGAAGGTTGGTAAATTCCTTG- CGGCTTTGGCAGCTATCCTGACGCTTGCGTATATTCTTG CGGTATACCCTCAAGTAGCACTAGTAGTAGTTGGCGCTTGTTACTTAGCGGCAGTGTGTGCTTGCGTGTGGAG- TATAGTTAACTGGTAATACGACTCACTAAAGGAGGTA CACACCATGATGTACTTAATGCCATTACTCATCGTCATTGTAGGATGCCTTGCGCTCCACTGTAGCGATGATG- ATATGCCAGATGGTCACGCTTAATACGACTCACTAAAG GAGACACTATATGTTTCGACTTCATTACAACAAAAGCGTTAAGAATTTCACGGTTCGCCGTGCTGACCGTTCA- ATCGTATGTGCGAGCGAGCGCCGAGCTAAGATACCTC TTATTGGTAACACAGTTCCTTTGGCACCGAGCGTCCACATCATTATCACCCGTGGTGACTTTGAGAAAGCAAT- AGACAAGAAACGTCCGGTTCTTAGTGTGGCAGTGACC CGCTTCCCGTTCGTCCGTCTGTTACTCAAACGAATCAAGGAGGTGTTCTGATGGGACTGTTAGATGGTGAAGC- CTGGGAAAAAGAAAACCCGCCAGTACAAGCAACTGG GTGTATAGCTTGCTTAGAGAAAGATGACCGTTATCCACACACCTGTAACAAAGGAGCTAACGATATGACCGAA- CGTGAACAAGAGATGATCATTAAGTTGATAGACAAT AATGAAGGTCGCCCAGATGATTTGAATGGCTGCGGTATTCTCTGCTCCAATGTCCCTTGCCACCTCTGCCCCG- CAAATAACGATCAAAAGATAACCTTAGGTGAAATCCG AGCGATGGACCCACGTAAACCACATCTGAATAAACCTGAGGTAACTCCTACAGATGACCAGCCTTCCGCTGAG- ACAATCGAAGGTGTCACTAAGCCTTCCCACTACATGC TGTTTGACGACATTGAGGCTATCGAAGTGATTGCTCGTTCAATGACCGTTGAGCAGTTCAAGGGATACTGCTT- CGGTAACATCTTAAAGTACAGACTACGTGCTGGTAAG AAGTCAGAGTTAGCGTACTTAGAGAAAGACCTAGCGAAAGCAGACTTCTATAAAGAACTCTTTGAGAAACATA- AGGATAAATGTTATGCATAACTTCAAGTCAACCCCA CCTGCCGACAGCCTATCTGATGACTTCACATCTTGCTCAGAGTGGTGCCGAAAGATGTGGGAAGAGACATTCG- ACGATGCGTACATCAAGCTGTATGAACTTTGGAAATC GAGAGGTCAATGACTATGTCAAACGTAAATACAGGTTCACTTAGTGTGGACAATAAGAAGTTTTGGGCTACCG- TAGAGTCCTCGGAGCATTCCTTCGAGGTTCCAATCTA CGCTGAGACCCTAGACGAAGCTCTGGAGTTAGCCGAATGGCAATACGTTCCGGCTGGCTTTGAGGTTACTCGT- GTGCGTCCTTGTGTAGCACCGAAGTAATACGACTCAC TATTAGGGAAGACTCCCTCTGAGAAACCAAACGAAACCTAAAGGAGATTAACATTATGGCTAAGAAGATTTTC- ACCTCTGCGCTGGGTACCGCTGAACCTTACGCTTACA TCGCCAAGCCGGACTACGGCAACGAAGAGCGTGGCTTTGGGAACCCTCGTGGTGTCTATAAAGTTGACCTGAC- TATTCCCAACAAAGACCCGCGCTGCCAGCGTATGGTC GATGAAATCGTGAAGTGTCACGAAGAGGCTTATGCTGCTGCCGTTGAGGAATACGAAGCTAATCCACCTGCTG- TAGCTCGTGGTAAGAAACCGCTGAAACCGTATGAGG GTGACATGCCGTTCTTCGATAACGGTGACGGTACGACTACCTTTAAGTTCAAATGCTACGCGTCTTTCCAAGA- CAAGAAGACCAAAGAGACCAAGCACATCAATCTGGTT GTGGTTGACTCAAAAGGTAAGAAGATGGAAGACGTTCCGATTATCGGTGGTGGCTCTAAGCTGAAAGTTAAAT- ATTCTCTGGTTCCATACAAGTGGAACACTGCTGTAGG TGCGAGCGTTAAGCTGCAACTGGAATCCGTGATGCTGGTCGAACTGGCTACCTTTGGTGGCGGTGAAGACGAT- TGGGCTGACGAAGTTGAAGAGAACGGCTATGTTGCC TCTGGTTCTGCCAAAGCGAGCAAACCACGCGACGAAGAAAGCTGGGACGAAGACGACGAAGAGTCCGAGGAAG- CAGACGAAGACGGAGACTTCTAAGTGGAACTGCG GGAGAAAATCCTTGAGCGAATCAAGGTGACTTCCTCTGGGTGTTGGGAGTGGCAGGGCGCTACGAACAATAAA- GGGTACGGGCAGGTGTGGTGCAGCAATACCGGAAA GGTTGTCTACTGTCATCGCGTAATGTCTAATGCTCCGAAAGGTTCTACCGTCCTGCACTCCTGTGATAATCCA- TTATGTTGTAACCCTGAACACCTATCCATAGGAACTCC AAAAGAGAACTCCACTGACATGGTAAATAAGGGTCGCTCACACAAGGGGTATAAACTTTCAGACGAAGACGTA- ATGGCAATCATGGAGTCCAGCGAGTCCAATGTATCC TTAGCTCGCACCTATGGTGTCTCCCAACAGACTATTTGTGATATACGCAAAGGGAGGCGACATGGCAGGTTAC- GGCGCTAAAGGAATCCGAAAGGTTGGAGCGTTTCGCT CTGGCCTAGAGGACAAGGTTTCAAAGCAGTTGGAATCAAAAGGTATTAAATTCGAGTATGAAGAGTGGAAAGT- GCCTTATGTAATTCCGGCGAGCAATCACACTTACAC TCCAGACTTCTTACTTCCAAACGGTATATTCGTTGAGACAAAGGGTCTGTGGGAAAGCGATGATAGAAAGAAG- CACTTATTAATTAGGGAGCAGCACCCCGAGCTAGAC ATCCGTATTGTCTTCTCAAGCTCACGTACTAAGTTATACAAAGGTTCTCCAACGTCTTATGGAGAGTTCTGCG- AAAAGCATGGTATTAAGTTCGCTGATAAACTGATACCT GCTGAGTGGATAAAGGAACCCAAGAAGGAGGTCCCCTTTGATAGATTAAAAAGGAAAGGAGGAAAGAAATAAT- GGCTCGTGTACAGTTTAAACAACGTGAATCTACTG ACGCAATCTTTGTTCACTGCTCGGCTACCAAGCCAAGTCAGAATGTTGGTGTCCGTGAGATTCGCCAGTGGCA- CAAAGAGCAGGGTTGGCTCGATGTGGGATACCACTTT ATCATCAAGCGAGACGGTACTGTGGAGGCAGGACGAGATGAGATGGCTGTAGGCTCTCACGCTAAGGGTTACA- ACCACAACTCTATCGGCGTCTGCCTTGTTGGTGGTAT CGACGATAAAGGTAAGTTCGACGCTAACTTTACGCCAGCCCAAATGCAATCCCTTCGCTCACTGCTTGTCACA- CTGCTGGCTAAGTACGAAGGCGCTGTGCTTCGCGCCC ATCATGAGGTGGCGCCGAAGGCTTGCCCTTCGTTCGACCTTAAGCGTTGGTGGGAGAAGAACGAACTGGTCAC- TTCTGACCGTGGATAATTAATTGAACTCACTAAAGGG AGACCACAGCGGTTTCCCTTTGTTCGCATTGGAGGTCAAATAATGCGCAAGTCTTATAAACAATTCTATAAGG- CTCCGAGGAGGCATATCCAAGTGTGGGAGGCAGCCAA TGGGCCTATACCAAAAGGTTATTATATAGACCACATTGACGGCAATCCACTCAACGACGCCTTAGACAATCTC- CGTCTGGCTCTCCCAAAAGAAAACTCATGGAACATGA AGACTCCAAAGAGCAATACCTCAGGACTAAAGGGACTGAGTTGGAGCAAGGAAAGGGAGATGTGGAGAGGCAC- TGTAACAGCTGAGGGTAAACAGCATAACTTTCGTA GTAGAGATCTATTGGAAGTCGTTGCGTGGATTTATAGAACTAGGAGGGAATTGCATGGACAATTCGCACGATT- CCGATAGTGTATTTCTTTACCACATTCCTTGTGACAAC TGTGGGAGTAGTGATGGGAACTCGCTGTTCTCTGACGGACACACGTTCTGCTACGTATGCGAGAAGTGGACTG- CTGGTAATGAAGACACTAAAGAGAGGGCTTCAAAAC GGAAACCCTCAGGAGGTAAACCAATGACTTACAACGTGTGGAACTTCGGGGAATCCAATGGACGCTACTCCGC- GTTAACTGCGAGAGGAATCTCCAAGGAAACCTGTCA GAAGGCTGGCTACTGGATTGCCAAAGTAGACGGTGTGATGTACCAAGTGGCTGACTATCGGGACCAGAACGGC- AACATTGTGAGTCAGAAGGTTCGAGATAAAGATAA GAACTTTAAGACCACTGGTAGTCACAAGAGTGACGCTCTGTTCGGGAAGCACTTGTGGAATGGTGGTAAGAAG- ATTGTCGTTACAGAAGGTGAAATCGACATGCTTACC GTGATGGAACTTCAAGACTGTAAGTATCCTGTAGTGTCGTTGGGTCACGGTGCCTCTGCCGCTAAGAAGACAT- GCGCTGCCAACTACGAATACTTTGACCAGTTCGAACA GATTATCTTAATGTTCGATATGGACGAAGCAGGGCGCAAAGCAGTCGAAGAGGCTGCACAGGTTCTACCTGCT- GGTAAGGTACGAGTGGCAGTTCTTCCGTGTAAGGAT GCAAACGAGTGTCACCTAAATGGTCACGACCGTGAAATCATGGAGCAAGTGTGGAATGCTGGTCCTTGGATTC- CTGATGGTGTGGTATCGGCTCTTTCGTTACGTGAACG AATCCGTGAGCACCTATCGTCCGAGGAATCAGTAGGTTTACTTTTCAGTGGCTGCACTGGTATCAACGATAAG- ACCTTAGGTGCCCGTGGTGGTGAAGTCATTATGGTCA CTTCCGGTTCCGGTATGGGTAAGTCAACGTTCGTCCGTCAACAAGCTCTACAATGGGGCACAGCGATGGGCAA- GAAGGTAGGCTTAGCGATGCTTGAGGAGTCCGTTGA GGAGACCGCTGAGGACCTTATAGGTCTACACAACCGTGTCCGACTGAGACAATCCGACTCACTAAAGAGAGAG- ATTATTGAGAACGGTAAGTTCGACCAATGGTTCGAT GAACTGTTCGGCAACGATACGTTCCATCTATATGACTCATTCGCCGAGGCTGAGACGGATAGACTGCTCGCTA- AGCTGGCCTACATGCGCTCAGGCTTGGGCTGTGACGT AATCATTCTAGACCACATCTCAATCGTCGTATCCGCTTCTGGTGAATCCGATGAGCGTAAGATGATTGACAAC- CTGATGACCAAGCTCAAAGGGTTCGCTAAGTCAACTG GGGTGGTGCTGGTCGTAATTTGTCACCTTAAGAACCCAGACAAAGGTAAAGCACATGAGGAAGGTCGCCCCGT- TTCTATTACTGACCTACGTGGTTCTGGCGCACTACGC CAACTATCTGATACTATTATTGCCCTTGAGCGTAATCAGCAAGGCGATATGCCTAACCTTGTCCTCGTTCGTA- TTCTCAAGTGCCGCTTTACTGGTGATACTGGTATCGCTG GCTACATGGAATACAACAAGGAAACCGGATGGCTTGAACCATCAAGTTACTCAGGGGAAGAAGAGTCACACTC- AGAGTCAACAGACTGGTCCAACGACACTGACTTCTG ACAGGATTCTTGATGACTTTCCAGACGACTACGAGAAGTTTCGCTGGAGAGTCCCATTCTAATACGACTCACT- AAAGGAGACACACCATGTTCAAACTGATTAAGAAGTT AGGCCAACTGCTGGTTCGTATGTACAACGTGGAAGCCAAGCGACTGAACGATGAGGCTCGTAAAGAGGCCACA-

CAGTCACGCGCTCTGGCGATTCGCTCCAACGAACTG GCTGACAGTGCATCCACTAAAGTTACCGAGGCTGCCCGTGTGGCAAACCAAGCTCAACAGCTTTCCAAATTCT- TTGAGTAATCAAACAGGAGAAACCATTATGTCTAACG TAGCTGAAACTATCCGTCTATCCGATACAGCTGACCAGTGGAACCGTCGAGTCCACATCAACGTTCGCAACGG- TAAGGCGACTATGGTTTACCGCTGGAAGGACTCTAAG TCCTCTAAGAATCACACTCAGCGTATGACGTTGACAGATGAGCAAGCACTGCGTCTGGTCAATGCGCTTACCA- AAGCTGCCGTGACAGCAATTCATGAAGCTGGTCGCGT CAATGAAGCTATGGCTATCCTCGACAAGATTGATAACTAAGAGTGGTATCCTCAAGGTCGCCAAAGTGGTGGC- CTTCATGAATACTATTCGACTCACTATAGGAGATATT ACCATGCGTGACCCTAAAGTTATCCAAGCAGAAATCGCTAAACTGGAAGCTGAACTGGAGGACGTTAAGTACC- ATGAAGCTAAGACTCGCTCCGCTGTTCACATCTTGA AGAACTTAGGCTGGACTTGGACAAGACAGACTGGCTGGAAGAAACCAGAAGTTACCAAGCTGAGTCATAAGGT- GTTCGATAAGGACACTATGACCCACATCAAGGCTG GTGATTGGGTTAAGGTTGACATGGGAGTTGTTGGTGGATACGGCTACGTCCGCTCAGTTAGTGGCAAATATGC- ACAAGTGTCATACATCACAGGTGTTACTCCACGCGGT GCAATCGTTGCCGATAAGACCAACATGATTCACACAGGTTTCTTGACAGTTGTTTCATATGAAGAGATTGTTA- AGTCACGATAATCAATAGGAGAAATCAATATGATCGT TTCTGACATCGAAGCTAACGCCCTCTTAGAGAGCGTCACTAAGTTCCACTGCGGGGTTATCTACGACTACTCC- ACCGCTGAGTACGTAAGCTACCGTCCGAGTGACTTCG GTGCGTATCTGGATGCGCTGGAAGCCGAGGTTGCACGAGGCGGTCTTATTGTGTTCCACAACGGTCACAAGTA- TGACGTTCCTGCATTGACCAAACTGGCAAAGTTGCAA TTGAACCGAGAGTTCCACCTTCCTCGTGAGAACTGTATTGACACCCTTGTGTTGTCACGTTTGATTCATTCCA- ACCTCAAGGACACCGATATGGGTCTTCTGCGTTCCGGC AAGTTGCCCGGAAAACGCTTTGGGTCTCACGCTTTGGAGGCGTGGGGTTATCGCTTAGGCGAGATGAAGGGTG- AATACAAAGACGACTTTAAGCGTATGCTTGAAGAGC AGGGTGAAGAATACGTTGACGGAATGGAGTGGTGGAACTTCAACGAAGAGATGATGGACTATAACGTTCAGGA- CGTTGTGGTAACTAAAGCTCTCCTTGAGAAGCTACT CTCTGACAAACATTACTTCCCTCCTGAGATTGACTTTACGGACGTAGGATACACTACGTTCTGGTCAGAATCC- CTTGAGGCCGTTGACATTGAACATCGTGCTGCATGGCT GCTCGCTAAACAAGAGCGCAACGGGTTCCCGTTTGACACAAAAGCAATCGAAGAGTTGTACGTAGAGTTAGCT- GCTCGCCGCTCTGAGTTGCTCCGTAAATTGACCGAA ACGTTCGGCTCGTGGTATCAGCCTAAAGGTGGCACTGAGATGTTCTGCCATCCGCGAACAGGTAAGCCACTAC- CTAAATACCCTCGCATTAAGACACCTAAAGTTGGTGG TATCTTTAAGAAGCCTAAGAACAAGGCACAGCGAGAAGGCCGTGAGCCTTGCGAACTTGATACCCGCGAGTAC- GTTGCTGGTGCTCCTTACACCCCAGTTGAACATGTTG TGTTTAACCCTTCGTCTCGTGACCACATTCAGAAGAAACTCCAAGAGGCTGGGTGGGTCCCGACCAAGTACAC- CGATAAGGGTGCTCCTGTGGTGGACGATGAGGTACTC GAAGGAGTACGTGTAGATGACCCTGAGAAGCAAGCCGCTATCGACCTCATTAAAGAGTACTTGATGATTCAGA- AGCGAATCGGACAGTCTGCTGAGGGAGACAAAGCAT GGCTTCGTTATGTTGCTGAGGATGGTAAGATTCATGGTTCTGTTAACCCTAATGGAGCAGTTACGGGTCGTGC- GACCCATGCGTTCCCAAACCTTGCGCAAATTCCGGGTG TACGTTCTCCTTATGGAGAGCAGTGTCGCGCTGCTTTTGGCGCTGAGCACCATTTGGATGGGATAACTGGTAA- GCCTTGGGTTCAGGCTGGCATCGACGCATCCGGTCTTG AGCTACGCTGCTTGGCTCACTTCATGGCTCGCTTTGATAACGGCGAGTACGCTCACGAGATTCTTAACGGCGA- CATCCACACTAAGAACCAGATAGCTGCTGAACTACCT ACCCGAGATAACGCTAAGACGTTCATCTATGGGTTCCTCTATGGTGCTGGTGATGAGAAGATTGGACAGATTG- TTGGTGCTGGTAAAGAGCGCGGTAAGGAACTCAAGA AGAAATTCCTTGAGAACACCCCCGCGATTGCAGCACTCCGCGAGTCTATCCAACAGACACTTGTCGAGTCCTC- TCAATGGGTAGCTGGTGAGCAACAAGTCAAGTGGAA ACGCCGCTGGATTAAAGGTCTGGATGGTCGTAAGGTACACGTTCGTAGTCCTCACGCTGCCTTGAATACCCTA- CTGCAATCTGCTGGTGCTCTCATCTGCAAACTGTGGAT TATCAAGACCGAAGAGATGCTCGTAGAGAAAGGCTTGAAGCATGGCTGGGATGGGGACTTTGCGTACATGGCA- TGGGTACATGATGAAATCCAAGTAGGCTGCCGTACC GAAGAGATTGCTCAGGTGGTCATTGAGACCGCACAAGAAGCGATGCGCTGGGTTGGAGACCACTGGAACTTCC- GGTGTCTTCTGGATACCGAAGGTAAGATGGGTCCTA ATTGGGCGATTTGCCACTGATACAGGAGGCTACTCATGAACGAAAGACACTTAACAGGTGCTGCTTCTGAAAT- GCTAGTAGCCTACAAATTTACCAAAGCTGGGTACACT GTCTATTACCCTATGCTGACTCAGAGTAAAGAGGACTTGGTTGTATGTAAGGATGGTAAATTTAGTAAGGTTC- AGGTTAAAACAGCCACAACGGTTCAAACCAACACAG GAGATGCCAAGCAGGTTAGGCTAGGTGGATGCGGTAGGTCCGAATATAAGGATGGAGACTTTGACATTCTTGC- GGTTGTGGTTGACGAAGATGTGCTTATTTTCACATGG GACGAAGTAAAAGGTAAGACATCCATGTGTGTCGGCAAGAGAAACAAAGGCATAAAACTATAGGAGAAATTAT- TATGGCTATGACAAAGAAATTTAAAGTGTCCTTCG ACGTTACCGCAAAGATGTCGTCTGACGTTCAGGCAATCTTAGAGAAAGATATGCTGCATCTATGTAAGCAGGT- CGGCTCAGGTGCGATTGTCCCCAATGGTAAACAGAA GGAAATGATTGTCCAGTTCCTGACACACGGTATGGAAGGATTGATGACATTCGTAGTACGTACATCATTTCGT- GAGGCCATTAAGGACATGCACGAAGAGTATGCAGAT AAGGACTCTTTCAAACAATCTCCTGCAACAGTACGGGAGGTGTTCTGATGTCTGACTACCTGAAAGTGCTGCA- AGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAAC TATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCACATCTCGTGCCTGACTACTAGTG- GACGTAACGGTGGCGCTTGGGAAATCACTGCTTCCGG TACTCGCTTTCTGAAACGAATGGGAGGATGTGTCTAATGTCTCGTGACCTTGTGACTATTCCACGCGATGTGT- GGAACGATATACAGGGCTACATCGACTCTCTGGAACG TGAGAACGATAGCCTTAAGAATCAACTAATGGAAGCTGACGAATACGTAGCGGAACTAGAGGAGAAACTTAAT- GGCACTTCTTGACCTTAAACAATTCTATGAGTTACG TGAAGGCTGCGACGACAAGGGTATCCTTGTGATGGACGGCGACTGGCTGGTCTTCCAAGCTATGAGTGCTGCT- GAGTTTGATGCCTCTTGGGAGGAAGAGATTTGGCACC GATGCTGTGACCACGCTAAGGCCCGTCAGATTCTTGAGGATTCCATTAAGTCCTACGAGACCCGTAAGAAGGC- TTGGGCAGGTGCTCCAATTGTCCTTGCGTTCACCGAT AGTGTTAACTGGCGTAAAGAACTGGTTGACCCGAACTATAAGGCTAACCGTAAGGCCGTGAAGAAACCTGTAG- GGTACTTTGAGTTCCTTGATGCTCTCTTTGAGCGCGA AGAGTTCTATTGCATCCGTGAGCCTATGCTTGAGGGTGATGACGTTATGGGAGTTATTGCTTCCAATCCGTCT- GCCTTCGGTGCTCGTAAGGCTGTAATCATCTCTTGCGA TAAGGACTTTAAGACCATCCCTAACTGTGACTTCCTGTGGTGTACCACTGGTAACATCCTGACTCAGACCGAA- GAGTCCGCTGACTGGTGGCACCTCTTCCAGACCATCA AGGGTGACATCACTGATGGTTACTCAGGGATTGCTGGATGGGGTGATACCGCCGAGGACTTCTTGAATAACCC- GTTCATAACCGAGCCTAAAACGTCTGTGCTTAAGTCC GGTAAGAACAAAGGCCAAGAGGTTACTAAATGGGTTAAACGCGACCCTGAGCCTCATGAGACGCTTTGGGACT- GCATTAAGTCCATTGGCGCGAAGGCTGGTATGACCG AAGAGGATATTATCAAGCAGGGCCAAATGGCTCGAATCCTACGGTTCAACGAGTACAACTTTATTGACAAGGA- GATTTACCTGTGGAGACCGTAGCGTATATTGGTCTGG GTCTTTGTGTTCTCGGAGTGTGCCTCATTTCGTGGGGCCTTTGGGACTTAGCCAGAATAATCAAGTCGTTACA- CGACACTAAGTGATAAACTCAAGGTCCCTAAATTAATA CGACTCACTATAGGGAGATAGGGGCCTTTACGATTATTACTTTAAGATTTAACTCTAAGAGGAATCTTTATTA- TGTTAACACCTATTAACCAATTACTTAAGAACCCTAAC GATATTCCAGATGTACCTCGTGCAACCGCTGAGTATCTACAGGTTCGATTCAACTATGCGTACCTCGAAGCGT- CTGGTCATATAGGACTTATGCGTGCTAATGGTTGTAGT GAGGCCCACATCTTGGGTTTCATTCAGGGCCTACAGTATGCCTCTAACGTCATTGACGAGATTGAGTTACGCA- AGGAACAACTAAGAGATGATGGGGAGGATTGACACT ATGTGTTTCTCACCGAAAATTAAAACTCCGAAGATGGATACCAATCAGATTCGAGCCGTTGAGCCAGCGCCTC- TGACCCAAGAAGTGTCAAGCGTGGAGTTCGGTGGGTC TTCTGATGAGACGGATACCGAGGGCACCGAAGTGTCTGGACGCAAAGGCCTCAAGGTCGAACGTGATGATTCC- GTAGCGAAGTCTAAAGCCAGCGGCAATGGCTCCGCT CGTATGAAATCTTCCATCCGTAAGTCCGCATTTGGAGGTAAGAAGTGATGTCTGAGTTCACATGTGTGGAGGC- TAAGAGTCGCTTCCGTGCAATCCGGTGGACTGTGGAA CACCTTGGGTTGCCTAAAGGATTCGAAGGACACTTTGTGGGCTACAGCCTCTACGTAGACGAAGTGATGGACA- TGTCTGGTTGCCGTGAAGAGTACATTCTGGACTCTAC CGGAAAACATGTAGCGTACTTCGCGTGGTGCGTAAGCTGTGACATTCACCACAAAGGAGACATTCTGGATGTA- ACGTCCGTTGTCATTAATCCTGAGGCAGACTCTAAGG GCTTACAGCGATTCCTAGCGAAACGCTTTAAGTACCTTGCGGAACTCCACGATTGCGATTGGGTGTCTCGTTG- TAAGCATGAAGGCGAGACAATGCGTGTATACTTTAAG GAGGTATAAGTTATGGGTAAGAAAGTTAAGAAGGCCGTGAAGAAAGTCACCAAGTCCGTTAAGAAAGTCGTTA- AGGAAGGGGCTCGTCCGGTTAAACAGGTTGCTGGC GGTCTAGCTGGTCTGGCTGGTGGTACTGGTGAAGCACAGATGGTGGAAGTACCACAAGCTGCCGCACAGATTG- TTGACGTACCTGAGAAAGAGGTTTCCACTGAGGACG AAGCACAGACAGAAAGCGGACGCAAGAAAGCTCGTGCTGGCGGTAAGAAATCCTTGAGTGTAGCCCGTAGCTC- CGGTGGCGGTATCAACATTTAATCAGGAGGTTATCG TGGAAGACTGCATTGAATGGACCGGAGGTGTCAACTCTAAGGGTTATGGTCGTAAGTGGGTTAATGGTAAACT- TGTGACTCCACATAGGCACATCTATGAGGAGACATA TGGTCCAGTTCCAACAGGAATTGTGGTGATGCATATCTGCGATAACCCTAGGTGCTATAACATAAAGCACCTT- ACGCTTGGAACTCCAAAGGATAATTCCGAGGACATGG TTACCAAAGGTAGACAGGCTAAAGGAGAGGAACTAAGCAAGAAACTTACAGAGTCAGACGTTCTCGCTATACG- CTCTTCAACCTTAAGCCACCGCTCCTTAGGAGAACT GTATGGAGTCAGTCAATCAACCATAACGCGAATACTACAGCGTAAGACATGGAGACACATTTAATGGCTGAGA- AACGAACAGGACTTGCGGAGGATGGCGCAAAGTCT GTCTATGAGCGTTTAAAGAACGACCGTGCTCCCTATGAGACACGCGCTCAGAATTGCGCTCAATATACCATCC- CATCATTGTTCCCTAAGGACTCCGATAACGCCTCTAC AGATTATCAAACTCCGTGGCAAGCCGTGGGCGCTCGTGGTCTGAACAATCTAGCCTCTAAGCTCATGCTGGCT- CTATTCCCTATGCAGACTTGGATGCGACTTACTATATC TGAATATGAAGCAAAGCAGTTACTGAGCGACCCCGATGGACTCGCTAAGGTCGATGAGGGCCTCTCGATGGTA- GAGCGTATCATCATGAACTACATTGAGTCTAACAGT TACCGCGTGACTCTCTTTGAGGCTCTCAAACAGTTAGTCGTAGCTGGTAACGTCCTGCTGTACCTACCGGAAC- CGGAAGGGTCAAACTATAATCCCATGAAGCTGTACCG ATTGTCTTCTTATGTGGTCCAACGAGACGCATTCGGCAACGTTCTGCAAATGGTGACTCGTGACCAGATAGCT- TTTGGTGCTCTCCCTGAGGACATCCGTAAGGCTGTAGA AGGTCAAGGTGGTGAGAAGAAAGCTGATGAGACAATCGACGTGTACACTCACATCTATCTGGATGAGGACTCA- GGTGAATACCTCCGATACGAAGAGGTCGAGGGTATG GAAGTCCAAGGCTCCGATGGGACTTATCCTAAAGAGGCTTGCCCATACATCCCGATTCGGATGGTCAGACTAG- ATGGTGAATCCTACGGTCGTTCGTACATTGAGGAATA CTTAGGTGACTTACGGTCCCTTGAAAATCTCCAAGAGGCTATCGTCAAGATGTCCATGATTAGCTCTAAGGTT- ATCGGCTTAGTGAATCCTGCTGGTATCACCCAGCCACG CCGACTGACCAAAGCTCAGACTGGTGACTTCGTTACTGGTCGTCCAGAAGACATCTCGTTCCTCCAACTGGAG- AAGCAAGCAGACTTTACTGTAGCTAAAGCCGTAAGTG ACGCTATCGAGGCTCGCCTTTCGTTTGCCTTTATGTTGAACTCTGCGGTTCAGCGTACAGGTGAACGTGTGAC- CGCCGAAGAGATTCGGTATGTAGCTTCTGAACTTGAAG ATACTTTAGGTGGTGTCTACTCTATCCTTTCTCAAGAATTACAATTGCCTCTGGTACGAGTGCTCTTGAAGCA- ACTACAAGCCACGCAACAGATTCCTGAGTTACCTAAGG AAGCCGTAGAGCCAACCATTAGTACAGGTCTGGAAGCAATTGGTCGAGGACAAGACCTTGATAAGCTGGAGCG- GTGTGTCACTGCGTGGGCTGCACTGGCACCTATGCG GGACGACCCTGATATTAACCTTGCGATGATTAAGTTACGTATTGCCAACGCTATCGGTATTGACACTTCTGGT- ATTCTACTCACCGAAGAACAGAAGCAACAGAAGATGG CCCAACAGTCTATGCAAATGGGTATGGATAATGGTGCTGCTGCGCTGGCTCAAGGTATGGCTGCACAAGCTAC- AGCTTCACCTGAGGCTATGGCTGCTGCCGCTGATTCC GTAGGTTTACAGCCGGGAATTTAATACGACTCACTATAGGGAGACCTCATCTTTGAAATGAGCGATGACAAGA- GGTTGGAGTCCTCGGTCTTCCTGTAGTTCAACTTTAA GGAGACAATAATAATGGCTGAATCTAATGCAGACGTATATGCATCTTTTGGCGTGAACTCCGCTGTGATGTCT- GGTGGTTCCGTTGAGGAACATGAGCAGAACATGCTGG CTCTTGATGTTGCTGCCCGTGATGGCGATGATGCAATCGAGTTAGCGTCAGACGAAGTGGAAACAGAACGTGA- CCTGTATGACAACTCTGACCCGTTCGGTCAAGAGGAT GACGAAGGCCGCATTCAGGTTCGTATCGGTGATGGCTCTGAGCCGACCGATGTGGACACTGGAGAAGAAGGCG- TTGAGGGCACCGAAGGTTCCGAAGAGTTTACCCCAC TGGGCGAGACTCCAGAAGAACTGGTAGCTGCCTCTGAGCAACTTGGTGAGCACGAAGAGGGCTTCCAAGAGAT- GATTAACATTGCTGCTGAGCGTGGCATGAGTGTCGA GACCATTGAGGCTATCCAGCGTGAGTACGAGGAGAACGAAGAGTTGTCCGCCGAGTCCTACGCTAAGCTGGCT- GAAATTGGCTACACGAAGGCTTTCATTGACTCGTAT ATCCGTGGTCAAGAAGCTCTGGTGGAGCAGTACGTAAACAGTGTCATTGAGTACGCTGGTGGTCGTGAACGTT- TTGATGCACTGTATAACCACCTTGAGACGCACAACCC TGAGGCTGCACAGTCGCTGGATAATGCGTTGACCAATCGTGACTTAGCGACCGTTAAGGCTATCATCAACTTG- GCTGGTGAGTCTCGCGCTAAGGCGTTCGGTCGTAAGC CAACTCGTAGTGTGACTAATCGTGCTATTCCGGCTAAACCTCAGGCTACCAAGCGTGAAGGCTTTGCGGACCG- TAGCGAGATGATTAAAGCTATGAGTGACCCTCGGTAT CGCACAGATGCCAACTATCGTCGTCAAGTCGAACAGAAAGTAATCGATTCGAACTTCTGATAGACTTCGAAAT- TAATACGACTCACTATAGGGAGACCACAACGGTTTCC CTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTAGCATGACTGGTGGACAGCAAATG- GGTACTAACCAAGGTAAAGGTGTAGTTGCTGCTGGAG ATAAACTGGCGTTGTTCTTGAAGGTATTTGGCGGTGAAGTCCTGACTGCGTTCGCTCGTACCTCCGTGACCAC- TTCTCGCCACATGGTACGTTCCATCTCCAGCGGTAAAT CCGCTCAGTTCCCTGTTCTGGGTCGCACTCAGGCAGCGTATCTGGCTCCGGGCGAGAACCTCGACGATAAACG- TAAGGACATCAAACACACCGAGAAGGTAATCACCAT TGACGGTCTCCTGACGGCTGACGTTCTGATTTATGATATTGAGGACGCGATGAACCACTACGACGTTCGCTCT- GAGTATACCTCTCAGTTGGGTGAATCTCTGGCGATGGC TGCGGATGGTGCGGTTCTGGCTGAGATTGCCGGTCTGTGTAACGTGGAAAGCAAATATAATGAGAACATCGAG- GGCTTAGGTACTGCTACCGTAATTGAGACCACTCAG AACAAGGCCGCACTTACCGACCAAGTTGCGCTGGGTAAGGAGATTATTGCGGCTCTGACTAAGGCTCGTGCGG- CTCTGACCAAGAACTATGTTCCGGCTGCTGACCGTGT GTTCTACTGTGACCCAGATAGCTACTCTGCGATTCTGGCAGCACTGATGCCGAACGCAGCAAACTACGCTGCT- CTGATTGACCCTGAGAAGGGTTCTATCCGCAACGTTA TGGGCTTTGAGGTTGTAGAAGTTCCGCACCTCACCGCTGGTGGTGCTGGTACCGCTCGTGAGGGCACTACTGG- TCAGAAGCACGTCTTCCCTGCCAATAAAGGTGAGGGT AATGTCAAGGTTGCTAAGGACAACGTTATCGGCCTGTTCATGCACCGCTCTGCGGTAGGTACTGTTAAGCTGC- GTGACTTGGCTCTGGAGCGCGCTCGCCGTGCTAACTTC CAAGCGGACCAGATTATCGCTAAGTACGCAATGGGCCACGGTGGTCTTCGCCCAGAAGCTGCTGGTGCAGTGG- TTTTCAAAGTGGAGTAATGCTGGGGGTGGCCTCAAC GGTCGCTGCTAGTCCCGAAGAGGCGAGTGTTACTTCAACAGAAGAAACCTTAACGCCAGCACAGGAGGCCGCA- CGCACCCGCGCTGCTAACAAAGCCCGAAAGGAAGC TGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGT- TTTTTGCTGAAAGGAGGAACTATATGCGCTCATACGA TATGAACGTTGAGACTGCCGCTGAGTTATCAGCTGTGAACGACATTCTGGCGTCTATCGGTGAACCTCCGGTA- TCAACGCTGGAAGGTGACGCTAACGCAGATGCAGCGA ACGCTCGGCGTATTCTCAACAAGATTAACCGACAGATTCAATCTCGTGGATGGACGTTCAACATTGAGGAAGG- CATAACGCTACTACCTGATGTTTACTCCAACCTGATT GTATACAGTGACGACTATTTATCCCTAATGTCTACTTCCGGTCAATCCATCTACGTTAACCGAGGTGGCTATG- TGTATGACCGAACGAGTCAATCAGACCGCTTTGACTCT GGTATTACTGTGAACATTATTCGTCTCCGCGACTACGATGAGATGCCTGAGTGCTTCCGTTACTGGATTGTCA- CCAAGGCTTCCCGTCAGTTCAACAACCGATTCTTTGGG GCACCGGAAGTAGAGGGTGTACTCCAAGAAGAGGAAGATGAGGCTAGACGTCTCTGCATGGAGTATGAGATGG- ACTACGGTGGGTACAATATGCTGGATGGAGATGCG TTCACTTCTGGTCTACTGACTCGCTAACATTAATAAATAAGGAGGCTCTAATGGCACTCATTAGCCAATCAAT- CAAGAACTTGAAGGGTGGTATCAGCCAACAGCCTGAC ATCCTTCGTTATCCAGACCAAGGGTCACGCCAAGTTAACGGTTGGTCTTCGGAGACCGAGGGCCTCCAAAAGC- GTCCACCTCTTGTTTTCTTAAATACACTTGGAGACAA CGGTGCGTTAGGTCAAGCTCCGTACATCCACCTGATTAACCGAGATGAGCACGAACAGTATTACGCTGTGTTC- ACTGGTAGCGGAATCCGAGTGTTCGACCTTTCTGGTA ACGAGAAGCAAGTTAGGTATCCTAACGGTTCCAACTACATCAAGACCGCTAATCCACGTAACGACCTGCGAAT- GGTTACTGTAGCAGACTATACGTTCATCGTTAACCGT AACGTTGTTGCACAGAAGAACACAAAGTCTGTCAACTTACCGAATTACAACCCTAATCAAGACGGATTGATTA- ACGTTCGTGGTGGTCAGTATGGTAGGGAACTAATTGT ACACATTAACGGTAAAGACGTTGCGAAGTATAAGATACCAGATGGTAGTCAACCTGAACACGTAAACAATACG- GATGCCCAATGGTTAGCTGAAGAGTTAGCCAAGCAG ATGCGCACTAACTTGTCTGATTGGACTGTAAATGTAGGGCAAGGGTTCATCCATGTGACCGCACCTAGTGGTC- AACAGATTGACTCCTTCACGACTAAAGATGGCTACGC AGACCAGTTGATTAACCCTGTGACCCACTACGCTCAGTCGTTCTCTAAGCTGCCACCTAATGCTCCTAACGGC- TACATGGTGAAAATCGTAGGGGACGCCTCTAAGTCTG CCGACCAGTATTACGTTCGGTATGACGCTGAGCGGAAAGTTTGGACTGAGACTTTAGGTTGGAACACTGAGGA- CCAAGTTCTATGGGAAACCATGCCACACGCTCTTGTG CGAGCCGCTGACGGTAATTTCGACTTCAAGTGGCTTGAGTGGTCTCCTAAGTCTTGTGGTGACGTTGACACCA- ACCCTTGGCCTTCTTTTGTTGGTTCAAGTATTAACGAT GTGTTCTTCTTCCGTAACCGCTTAGGATTCCTTAGTGGGGAGAACATCATATTGAGTCGTACAGCCAAATACT- TCAACTTCTACCCTGCGTCCATTGCGAACCTTAGTGAT GACGACCCTATAGACGTAGCTGTGAGTACCAACCGAATAGCAATCCTTAAGTACGCCGTTCCGTTCTCAGAAG- AGTTACTCATCTGGTCCGATGAAGCACAATTCGTCCT GACTGCCTCGGGTACTCTCACATCTAAGTCGGTTGAGTTGAACCTAACGACCCAGTTTGACGTACAGGACCGA- GCGAGACCTTTTGGGATTGGGCGTAATGTCTACTTTG CTAGTCCGAGGTCCAGCTTCACGTCCATCCACAGGTACTACGCTGTGCAGGATGTCAGTTCCGTTAAGAATGC- TGAGGACATTACATCACACGTTCCTAACTACATCCCTA ATGGTGTGTTCAGTATTTGCGGAAGTGGTACGGAAAACTTCTGTTCGGTACTATCTCACGGGGACCCTAGTAA- AATCTTCATGTACAAATTCCTGTACCTGAACGAAGAG TTAAGGCAACAGTCGTGGTCTCATTGGGACTTTGGGGAAAACGTACAGGTTCTAGCTTGTCAGAGTATCAGCT- CAGATATGTATGTGATTCTTCGCAATGAGTTCAATAC GTTCCTAGCTAGAATCTCTTTCACTAAGAACGCCATTGACTTACAGGGAGAACCCTATCGTGCCTTTATGGAC- ATGAAGATTCGATACACGATTCCTAGTGGAACATACA ACGATGACACATTCACTACCTCTATTCATATTCCAACAATTTATGGTGCAAACTTCGGGAGGGGCAAAATCAC- TGTATTGGAGCCTGATGGTAAGATAACCGTGTTTGAG CAACCTACGGCTGGGTGGAATAGCGACCCTTGGCTGAGACTCAGCGGTAACTTGGAGGGACGCATGGTGTACA- TTGGGTTCAACATTAACTTCGTATATGAGTTCTCTAA GTTCCTCATCAAGCAGACTGCCGACGACGGGTCTACCTCCACGGAAGACATTGGGCGCTTACAGTTACGCCGA- GCGTGGGTTAACTACGAGAACTCTGGTACGTTTGACA TTTATGTTGAGAACCAATCGTCTAACTGGAAGTACACAATGGCTGGTGCCCGATTAGGCTCTAACACTCTGAG- GGCTGGGAGACTGAACTTAGGGACCGGACAATATCG ATTCCCTGTGGTTGGTAACGCCAAGTTCAACACTGTATACATCTTGTCAGATGAGACTACCCCTCTGAACATC- ATTGGGTGTGGCTGGGAAGGTAACTACTTACGGAGAA

GTTCCGGTATTTAATTAAATATTCTCCCTGTGGTGGCTCGAAATTAATACGACTCACTATAGGGAGAACAATA- CGACTACGGGAGGGTTTTCTTATGATGACTATAAGAC CTACTAAAAGTACAGACTTTGAGGTATTCACTCCGGCTCACCATGACATTCTTGAAGCTAAGGCTGCTGGTAT- TGAGCCGAGTTTCCCTGATGCTTCCGAGTGTGTCACGT TGAGCCTCTATGGGTTCCCTCTAGCTATCGGTGGTAACTGCGGGGACCAGTGCTGGTTCGTTACGAGCGACCA- AGTGTGGCGACTTAGTGGAAAGGCTAAGCGAAAGTTC CGTAAGTTAATCATGGAGTATCGCGATAAGATGCTTGAGAAGTATGATACTCTTTGGAATTACGTATGGGTAG- GCAATACGTCCCACATTCGTTTCCTCAAGACTATCGG TGCGGTATTCCATGAAGAGTACACACGAGATGGTCAATTTCAGTTATTTACAATCACGAAAGGAGGATAACCA- TATGTGTTGGGCAGCCGCAATACCTATCGCTATATCT GGCGCTCAGGCTATCAGTGGTCAGAACGCTCAGGCCAAAATGATTGCCGCTCAGACCGCTGCTGGTCGTCGTC- AAGCTATGGAAATCATGAGGCAGACGAACATCCAGA ATGCTGACCTATCGTTGCAAGCTCGAAGTAAACTTGAGGAAGCGTCCGCCGAGTTGACCTCACAGAACATGCA- GAAGGTCCAAGCTATTGGGTCTATCCGAGCGGCTATC GGAGAGAGTATGCTTGAAGGTTCCTCAATGGACCGCATTAAGCGAGTCACAGAAGGACAGTTCATTCGGGAAG- CCAATATGGTAACTGAGAACTATCGCCGTGACTACC AAGCAATCTTCGCACAGCAACTTGGTGGTACTCAAAGTGCTGCAAGTCAGATTGACGAAATCTATAAGAGCGA- ACAGAAACAGAAGAGTAAGCTACAGATGGTTCTGGA CCCACTGGCTATCATGGGGTCTTCCGCTGCGAGTGCTTACGCATCCGGTGCGTTCGACTCTAAGTCCACAACT- AAGGCACCTATTGTTGCCGCTAAAGGAACCAAGACGG GGAGGTAATGAGCTATGAGTAAAATTGAATCTGCCCTTCAAGCGGCACAACCGGGACTCTCTCGGTTACGTGG- TGGTGCTGGAGGTATGGGCTATCGTGCAGCAACCACT CAGGCCGAACAGCCAAGGTCAAGCCTATTGGACACCATTGGTCGGTTCGCTAAGGCTGGTGCCGATATGTATA- CCGCTAAGGAACAACGAGCACGAGACCTAGCTGATG AACGCTCTAACGAGATTATCCGTAAGCTGACCCCTGAGCAACGTCGAGAAGCTCTCAACAACGGGACCCTTCT- GTATCAGGATGACCCATACGCTATGGAAGCACTCCG AGTCAAGACTGGTCGTAACGCTGCGTATCTTGTGGACGATGACGTTATGCAGAAGATAAAAGAGGGTGTCTTC- CGTACTCGCGAAGAGATGGAAGAGTATCGCCATAGT CGCCTTCAAGAGGGCGCTAAGGTATACGCTGAGCAGTTCGGCATCGACCCTGAGGACGTTGATTATCAGCGTG- GTTTCAACGGGGACATTACCGAGCGTAACATCTCGCT GTATGGTGCGCATGATAACTTCTTGAGCCAGCAAGCTCAGAAGGGCGCTATCATGAACAGCCGAGTGGAACTC- AACGGTGTCCTTCAAGACCCTGATATGCTGCGTCGTC CAGACTCTGCTGACTTCTTTGAGAAGTATATCGACAACGGTCTGGTTACTGGCGCAATCCCATCTGATGCTCA- AGCCACACAGCTTATAAGCCAAGCGTTCAGTGACGCT TCTAGCCGTGCTGGTGGTGCTGACTTCCTGATGCGAGTCGGTGACAAGAAGGTAACACTTAACGGAGCCACTA- CGACTTACCGAGAGTTGATTGGTGAGGAACAGTGGA ACGCTCTCATGGTCACAGCACAACGTTCTCAGTTTGAGACTGACGCGAAGCTGAACGAGCAGTATCGCTTGAA- GATTAACTCTGCGCTGAACCAAGAGGACCCAAGGAC AGCTTGGGAGATGCTTCAAGGTATCAAGGCTGAACTAGATAAGGTCCAACCTGATGAGCAGATGACACCACAA- CGTGAGTGGCTAATCTCCGCACAGGAACAAGTTCAG AATCAGATGAACGCATGGACGAAAGCTCAGGCCAAGGCTCTGGACGATTCCATGAAGTCAATGAACAAACTTG- ACGTAATCGACAAGCAATTCCAGAAGCGAATCAAC GGTGAGTGGGTCTCAACGGATTTTAAGGATATGCCAGTCAACGAGAACACTGGTGAGTTCAAGCATAGCGATA- TGGTTAACTACGCCAATAAGAAGCTCGCTGAGATTG ACAGTATGGACATTCCAGACGGTGCCAAGGATGCTATGAAGTTGAAGTACCTTCAAGCGGACTCTAAGGACGG- AGCATTCCGTACAGCCATCGGAACCATGGTCACTGA CGCTGGTCAAGAGTGGTCTGCCGCTGTGATTAACGGTAAGTTACCAGAACGAACCCCAGCTATGGATGCTCTG- CGCAGAATCCGCAATGCTGACCCTCAGTTGATTGCTG CGCTATACCCAGACCAAGCTGAGCTATTCCTGACGATGGACATGATGGACAAGCAGGGTATTGACCCTCAGGT- TATTCTTGATGCCGACCGACTGACTGTTAAGCGGTCC AAAGAGCAACGCTTTGAGGATGATAAAGCATTCGAGTCTGCACTGAATGCATCTAAGGCTCCTGAGATTGCCC- GTATGCCAGCGTCACTGCGCGAATCTGCACGTAAGAT TTATGACTCCGTTAAGTATCGCTCGGGGAACGAAAGCATGGCTATGGAGCAGATGACCAAGTTCCTTAAGGAA- TCTACCTACACGTTCACTGGTGATGATGTTGACGGTG ATACCGTTGGTGTGATTCCTAAGAATATGATGCAGGTTAACTCTGACCCGAAATCATGGGAGCAAGGTCGGGA- TATTCTGGAGGAAGCACGTAAGGGAATCATTGCGAG CAACCCTTGGATAACCAATAAGCAACTGACCATGTATTCTCAAGGTGACTCCATTTACCTTATGGACACCACA- GGTCAAGTCAGAGTCCGATACGACAAAGAGTTACTCT CGAAGGTCTGGAGTGAGAACCAGAAGAAACTCGAAGAGAAAGCTCGTGAGAAGGCTCTGGCTGATGTGAACAA- GCGAGCACCTATAGTTGCCGCTACGAAGGCCCGTG AAGCTGCTGCTAAACGAGTCCGAGAGAAACGTAAACAGACTCCTAAGTTCATCTACGGACGTAAGGAGTAACT- AAAGGCTACATAAGGAGGCCCTAAATGGATAAGTA CGATAAGAACGTACCAAGTGATTATGATGGTCTGTTCCAAAAGGCTGCTGATGCCAACGGGGTCTCTTATGAC- CTTTTACGTAAAGTCGCTTGGACAGAATCACGATTTG TGCCTACAGCAAAATCTAAGACTGGACCATTAGGCATGATGCAATTTACCAAGGCAACCGCTAAGGCCCTCGG- TCTGCGAGTTACCGATGGTCCAGACGACGACCGACT GAACCCTGAGTTAGCTATTAATGCTGCCGCTAAGCAACTTGCAGGTCTGGTAGGGAAGTTTGATGGCGATGAA- CTCAAAGCTGCCCTTGCGTACAACCAAGGCGAGGGA CGCTTGGGTAATCCACAACTTGAGGCGTACTCTAAGGGAGACTTCGCATCAATCTCTGAGGAGGGACGTAACT- ACATGCGTAACCTTCTGGATGTTGCTAAGTCACCTAT GGCTGGACAGTTGGAAACTTTTGGTGGCATAACCCCAAAGGGTAAAGGCATTCCGGCTGAGGTAGGATTGGCT- GGAATTGGTCACAAGCAGAAAGTAACACAGGAACTT CCTGAGTCCACAAGTTTTGACGTTAAGGGTATCGAACAGGAGGCTACGGCGAAACCATTCGCCAAGGACTTTT- GGGAGACCCACGGAGAAACACTTGACGAGTACAACA GTCGTTCAACCTTCTTCGGATTCAAAAATGCTGCCGAAGCTGAACTCTCCAACTCAGTCGCTGGGATGGCTTT- CCGTGCTGGTCGTCTCGATAATGGTTTTGATGTGTTTA AAGACACCATTACGCCGACTCGCTGGAACTCTCACATCTGGACTCCAGAGGAGTTAGAGAAGATTCGAACAGA- GGTTAAGAACCCTGCGTACATCAACGTTGTAACTGG TGGTTCCCCTGAGAACCTCGATGACCTCATTAAATTGGCTAACGAGAACTTTGAGAATGACTCCCGCGCTGCC- GAGGCTGGCCTAGGTGCCAAACTGAGTGCTGGTATTA TTGGTGCTGGTGTGGACCCGCTTAGCTATGTTCCTATGGTCGGTGTCACTGGTAAGGGCTTTAAGTTAATCAA- TAAGGCTCTTGTAGTTGGTGCCGAAAGTGCTGCTCTGA ACGTTGCATCCGAAGGTCTCCGTACCTCCGTAGCTGGTGGTGACGCAGACTATGCGGGTGCTGCCTTAGGTGG- CTTTGTGTTTGGCGCAGGCATGTCTGCAATCAGTGAC GCTGTAGCTGCTGGACTGAAACGCAGTAAACCAGAAGCTGAGTTCGACAATGAGTTCATCGGTCCTATGATGC- GATTGGAAGCCCGTGAGACAGCACGAAACGCCAACT CTGCGGACCTCTCTCGGATGAACACTGAGAACATGAAGTTTGAAGGTGAACATAATGGTGTCCCTTATGAGGA- CTTACCAACAGAGAGAGGTGCCGTGGTGTTACATGA TGGCTCCGTTCTAAGTGCAAGCAACCCAATCAACCCTAAGACTCTAAAAGAGTTCTCCGAGGTTGACCCTGAG- AAGGCTGCGCGAGGAATCAAACTGGCTGGGTTCACC GAGATTGGCTTGAAGACCTTGGGGTCTGACGATGCTGACATCCGTAGAGTGGCTATCGACCTCGTTCGCTCTC- CTACTGGTATGCAGTCTGGTGCCTCAGGTAAGTTCGGT GCAACAGCTTCTGACATCCATGAGAGACTTCATGGTACTGACCAGCGTACTTATAATGACTTGTACAAAGCAA- TGTCTGACGCTATGAAAGACCCTGAGTTCTCTACTGG CGGCGCTAAGATGTCCCGTGAAGAAACTCGATACACTATCTACCGTAGAGCGGCACTAGCTATTGAGCGTCCA- GAACTACAGAAGGCACTCACTCCGTCTGAGAGAATC GTTATGGACATCATTAAGCGTCACTTTGACACCAAGCGTGAACTTATGGAAAACCCAGCAATATTCGGTAACA- CAAAGGCTGTGAGTATCTTCCCTGAGAGTCGCCACAA AGGTACTTACGTTCCTCACGTATATGACCGTCATGCCAAGGCGCTGATGATTCAACGCTACGGTGCCGAAGGT- TTGCAGGAAGGGATTGCCCGCTCATGGATGAACAGCT ACGTCTCCAGACCTGAGGTCAAGGCCAGAGTCGATGAGATGCTTAAGGAATTACACGGGGTGAAGGAAGTAAC- ACCAGAGATGGTAGAGAAGTACGCTATGGATAAGG CTTATGGTATCTCCCACTCAGACCAGTTCACCAACAGTTCCATAATAGAAGAGAACATTGAGGGCTTAGTAGG- TATCGAGAATAACTCATTCCTTGAGGCACGTAACTTG TTTGATTCGGACCTATCCATCACTATGCCAGACGGACAGCAATTCTCAGTGAATGACCTAAGGGACTTCGATA- TGTTCCGCATCATGCCAGCGTATGACCGCCGTGTCAA TGGTGACATCGCCATCATGGGGTCTACTGGTAAAACCACTAAGGAACTTAAGGATGAGATTTTGGCTCTCAAA- GCGAAAGCTGAGGGAGACGGTAAGAAGACTGGCGAG GTACATGCTTTAATGGATACCGTTAAGATTCTTACTGGTCGTGCTAGACGCAATCAGGACACTGTGTGGGAAA- CCTCACTGCGTGCCATCAATGACCTAGGGTTCTTCGCT AAGAACGCCTACATGGGTGCTCAGAACATTACGGAGATTGCTGGGATGATTGTCACTGGTAACGTTCGTGCTC- TAGGGCATGGTATCCCAATTCTGCGTGATACACTCTA CAAGTCTAAACCAGTTTCAGCTAAGGAACTCAAGGAACTCCATGCGTCTCTGTTCGGGAAGGAGGTGGACCAG- TTGATTCGGCCTAAACGTGCTGACATTGTGCAGCGCC TAAGGGAAGCAACTGATACCGGACCTGCCGTGGCGAACATCGTAGGGACCTTGAAGTATTCAACACAGGAACT- GGCTGCTCGCTCTCCGTGGACTAAGCTACTGAACGG AACCACTAACTACCTTCTGGATGCTGCGCGTCAAGGTATGCTTGGGGATGTTATTAGTGCCACCCTAACAGGT- AAGACTACCCGCTGGGAGAAAGAAGGCTTCCTTCGTG GTGCCTCCGTAACTCCTGAGCAGATGGCTGGCATCAAGTCTCTCATCAAGGAACATATGGTACGCGGTGAGGA- CGGGAAGTTTACCGTTAAGGACAAGCAAGCGTTCTCT ATGGACCCACGGGCTATGGACTTATGGAGACTGGCTGACAAGGTAGCTGATGAGGCAATGCTGCGTCCACATA- AGGTGTCCTTACAGGATTCCCATGCGTTCGGAGCACT AGGTAAGATGGTTATGCAGTTTAAGTCTTTCACTATCAAGTCCCTTAACTCTAAGTTCCTGCGAACCTTCTAT- GATGGATACAAGAACAACCGAGCGATTGACGCTGCGCT GAGCATCATCACCTCTATGGGTCTCGCTGGTGGTTTCTATGCTATGGCTGCACACGTCAAAGCATACGCTCTG- CCTAAGGAGAAACGTAAGGAGTACTTGGAGCGTGCAC TGGACCCAACCATGATTGCCCACGCTGCGTTATCTCGTAGTTCTCAATTGGGTGCTCCTTTGGCTATGGTTGA- CCTAGTTGGTGGTGTTTTAGGGTTCGAGTCCTCCAAGAT GGCTCGCTCTACGATTCTACCTAAGGACACCGTGAAGGAACGTGACCCAAACAAACCGTACACCTCTAGAGAG- GTAATGGGCGCTATGGGTTCAAACCTTCTGGAACAG ATGCCTTCGGCTGGCTTTGTGGCTAACGTAGGGGCTACCTTAATGAATGCTGCTGGCGTGGTCAACTCACCTA- ATAAAGCAACCGAGCAGGACTTCATGACTGGTCTTAT GAACTCCACAAAAGAGTTAGTACCGAACGACCCATTGACTCAACAGCTTGTGTTGAAGATTTATGAGGCGAAC- GGTGTTAACTTGAGGGAGCGTAGGAAATAATACGAC TCACTATAGGGAGAGGCGAAATAATCTTCTCCCTGTAGTCTCTTAGATTTACTTTAAGGAGGTCAAATGGCTA- ACGTAATTAAAACCGTTTTGACTTACCAGTTAGATGGC TCCAATCGTGATTTTAATATCCCGTTTGAGTATCTAGCCCGTAAGTTCGTAGTGGTAACTCTTATTGGTGTAG- ACCGAAAGGTCCTTACGATTAATACAGACTATCGCTTT GCTACACGTACTACTATCTCTCTGACAAAGGCTTGGGGTCCAGCCGATGGCTACACGACCATCGAGTTACGTC- GAGTAACCTCCACTACCGACCGATTGGTTGACTTTAC GGATGGTTCAATCCTCCGCGCGTATGACCTTAACGTCGCTCAGATTCAAACGATGCACGTAGCGGAAGAGGCC- CGTGACCTCACTACGGATACTATCGGTGTCAATAACG ATGGTCACTTGGATGCTCGTGGTCGTCGAATTGTGAACCTAGCGAACGCCGTGGATGACCGCGATGCTGTTCC- GTTTGGTCAACTAAAGACCATGAACCAGAACTCATGG CAAGCACGTAATGAAGCCTTACAGTTCCGTAATGAGGCTGAGACTTTCAGAAACCAAGCGGAGGGCTTTAAGA- ACGAGTCCAGTACCAACGCTACGAACACAAAGCAGT GGCGCGATGAGACCAAGGGTTTCCGAGACGAAGCCAAGCGGTTCAAGAATACGGCTGGTCAATACGCTACATC- TGCTGGGAACTCTGCTTCCGCTGCGCATCAATCTGA GGTAAACGCTGAGAACTCTGCCACAGCATCCGCTAACTCTGCTCATTTGGCAGAACAGCAAGCAGACCGTGCG- GAACGTGAGGCAGACAAGCTGGAAAATTACAATGGA TTGGCTGGTGCAATTGATAAGGTAGATGGAACCAATGTGTACTGGAAAGGAAATATTCACGCTAACGGGCGCC- TTTACATGACCACAAACGGTTTTGACTGTGGCCAGTA TCAACAGTTCTTTGGTGGTGTCACTAATCGTTACTCTGTCATGGAGTGGGGAGATGAGAACGGATGGCTGATG- TATGTTCAACGTAGAGAGTGGACAACAGCGATAGGC GGTAACATCCAGTTAGTAGTAAACGGACAGATCATCACCCAAGGTGGAGCCATGACCGGTCAGCTAAAATTGC- AGAATGGGCATGTTCTTCAATTAGAGTCCGCATCCG ACAAGGCGCACTATATTCTATCTAAAGATGGTAACAGGAATAACTGGTACATTGGTAGAGGGTCAGATAACAA- CAATGACTGTACCTTCCACTCCTATGTACATGGTACG ACCTTAACACTCAAGCAGGACTATGCAGTAGTTAACAAACACTTCCACGTAGGTCAGGCCGTTGTGGCCACTG- ATGGTAATATTCAAGGTACTAAGTGGGGAGGTAAAT GGCTGGATGCTTACCTACGTGACAGCTTCGTTGCGAAGTCCAAGGCGTGGACTCAGGTGTGGTCTGGTAGTGC- TGGCGGTGGGGTAAGTGTGACTGTTTCACAGGATCTC CGCTTCCGCAATATCTGGATTAAGTGTGCCAACAACTCTTGGAACTTCTTCCGTACTGGCCCCGATGGAATCT- ACTTCATAGCCTCTGATGGTGGATGGTTACGATTCCAA ATACACTCCAACGGTCTCGGATTCAAGAATATTGCAGACAGTCGTTCAGTACCTAATGCAATCATGGTGGAGA- ACGAGTAATTGGTAAATCACAAGGAAAGACGTGTAG TCCACGGATGGACTCTCAAGGAGGTACAAGGTGCTATCATTAGACTTTAACAACGAATTGATTAAGGCTGCTC- CAATTGTTGGGACGGGTGTAGCAGATGTTAGTGCTCG ACTGTTCTTTGGGTTAAGCCTTAACGAATGGTTCTACGTTGCTGCTATCGCCTACACAGTGGTTCAGATTGGT- GCCAAGGTAGTCGATAAGATGATTGACTGGAAGAAAG CCAATAAGGAGTGATATGTATGGAAAAGGATAAGAGCCTTATTACATTCTTAGAGATGTTGGACACTGCGATG- GCTCAGCGTATGCTTGCGGACCTTTCGGACCATGAGC GTCGCTCTCCGCAACTCTATAATGCTATTAACAAACTGTTAGACCGCCACAAGTTCCAGATTGGTAAGTTGCA- GCCGGATGTTCACATCTTAGGTGGCCTTGCTGGTGCTC TTGAAGAGTACAAAGAGAAAGTCGGTGATAACGGTCTTACGGATGATGATATTTACACATTACAGTGATATAC- TCAAGGCCACTACAGATAGTGGTCTTTATGGATGTCA TTGTCTATACGAGATGCTCCTACGTGAAATCTGAAAGTTAACGGGAGGCATTATGCTAGAATTTTTACGTAAG- CTAATCCCTTGGGTTCTCGCTGGGATGCTATTCGGGTT AGGATGGCATCTAGGGTCAGACTCAATGGACGCTAAATGGAAACAGGAGGTACACAATGAGTACGTTAAGAGA- GTTGAGGCTGCGAAGAGCACTCAAAGAGCAATCGA TGCGGTATCTGCTAAGTATCAAGAAGACCTTGCCGCGCTGGAAGGGAGCACTGATAGGATTATTTCTGATTTG- CGTAGCGACAATAAGCGGTTGCGCGTCAGAGTCAAA ACTACCGGAACCTCCGATGGTCAGTGTGGATTCGAGCCTGATGGTCGAGCCGAACTTGACGACCGAGATGCTA- AACGTATTCTCGCAGTGACCCAGAAGGGTGACGCAT GGATTCGTGCGTTACAGGATACTATTCGTGAACTGCAACGTAAGTAGGAAATCAAGTAAGGAGGCAATGTGTC- TACTCAATCCAATCGTAATGCGCTCGTAGTGGCGCA ACTGAAAGGAGACTTCGTGGCGTTCCTATTCGTCTTATGGAAGGCGCTAAACCTACCGGTGCCCACTAAGTGT- CAGATTGACATGGCTAAGGTGCTGGCGAATGGAGACA ACAAGAAGTTCATCTTACAGGCTTTCCGTGGTATCGGTAAGTCGTTCATCACATGTGCGTTCGTTGTGTGGTC- CTTATGGAGAGACCCTCAGTTGAAGATACTTATCGTAT CAGCCTCTAAGGAGCGTGCAGACGCTAACTCCATCTTTATTAAGAACATCATTGACCTGCTGCCATTCCTATC- TGAGTTAAAGCCAAGACCCGGACAGCGTGACTCGGTA ATCAGCTTTGATGTAGGCCCAGCCAATCCTGACCACTCTCCTAGTGTGAAATCAGTAGGTATCACTGGTCAGT- TAACTGGTAGCCGTGCTGACATTATCATTGCGGATGAC GTTGAGATTCCGTCTAACAGCGCAACTATGGGTGCCCGTGAGAAGCTATGGACTCTGGTTCAGGAGTTCGCTG- CGTTACTTAAACCGCTGCCTTCCTCTCGCGTTATCTAC CTTGGTACACCTCAGACAGAGATGACTCTCTATAAGGAACTTGAGGATAACCGTGGGTACACAACCATTATCT- GGCCTGCTCTGTACCCAAGGACACGTGAAGAGAACCT CTATTACTCACAGCGTCTTGCTCCTATGTTACGCGCTGAGTACGATGAGAACCCTGAGGCACTTGCTGGGACT- CCAACAGACCCAGTGCGCTTTGACCGTGATGACCTGC GCGAGCGTGAGTTGGAATACGGTAAGGCTGGCTTTACGCTACAGTTCATGCTTAACCCTAACCTTAGTGATGC- CGAGAAGTACCCGCTGAGGCTTCGTGACGCTATCGTA GCGGCCTTAGACTTAGAGAAGGCCCCAATGCATTACCAGTGGCTTCCGAACCGTCAGAACATCATTGAGGACC- TTCCTAACGTTGGCCTTAAGGGTGATGACCTGCATAC GTACCACGATTGTTCCAACAACTCAGGTCAGTACCAACAGAAGATTCTGGTCATTGACCCTAGTGGTCGCGGT- AAGGACGAAACAGGTTACGCTGTGCTGTACACACTGA ACGGTTACATCTACCTTATGGAAGCTGGAGGTTTCCGTGATGGCTACTCCGATAAGACCCTTGAGTTACTCGC- TAAGAAGGCAAAGCAATGGGGAGTCCAGACGGTTGTC TACGAGAGTAACTTCGGTGACGGTATGTTCGGTAAGGTATTCAGTCCTATCCTTCTTAAACACCACAACTGTG- CGATGGAAGAGATTCGTGCCCGTGGTATGAAAGAGAT GCGTATTTGCGATACCCTTGAGCCAGTCATGCAGACTCACCGCCTTGTAATTCGTGATGAGGTCATTAGGGCC- GACTACCAGTCCGCTCGTGACGTAGACGGTAAGCATG ACGTTAAGTACTCGTTGTTCTACCAGATGACCCGTATCACTCGTGAGAAAGGCGCTCTGGCTCATGATGACCG- ATTGGATGCCCTTGCGTTAGGCATTGAGTATCTCCGTG AGTCCATGCAGTTGGATTCCGTTAAGGTCGAGGGTGAAGTACTTGCTGACTTCCTTGAGGAACACATGATGCG- TCCTACGGTTGCTGCTACGCATATCATTGAGATGTCTG TGGGAGGAGTTGATGTGTACTCTGAGGACGATGAGGGTTACGGTACGTCTTTCATTGAGTGGTGATTTATGCA- TTAGGACTGCATAGGGATGCACTATAGACCACGGATG GTCAGTTCTTTAAGTTACTGAAAAGACACGATAAATTAATACGACTCACTATAGGGAGAGGAGGGACGAAAGG- TTACTATATAGATACTGAATGAATACTTATAGAGTG CATAAAGTATGCATAATGGTGTACCTAGAGTGACCTCTAAGAATGGTGATTATATTGTATTAGTATCACCTTA- ACTTAAGGACCAACATAAAGGGAGGAGACTCATGTTC CGCTTATTGTTGAACCTACTGCGGCATAGAGTCACCTACCGATTTCTTGTGGTACTTTGTGCTGCCCTTGGGT- ACGCATCTCTTACTGGAGACCTCAGTTCACTGGAGTCTG TCGTTTGCTCTATACTCACTTGTAGCGATTAGGGTCTTCCTGACCGACTGATGGCTCACCGAGGGATTCAGCG- GTATGATTGCATCACACCACTTCATCCCTATAGAGTCA AGTCCTAAGGTATACCCATAAAGAGCCTCTAATGGTCTATCCTAAGGTCTATACCTAAAGATAGGCCATCCTA- TCAGTGTCACCTAAAGAGGGTCTTAGAGAGGGCCTAT GGAGTTCCTATAGGGTCCTTTAAAATATACCATAAAAATCTGAGTGACTATCTCACAGTGTACGGACCTAAAG- TTCCCCCATAGGGGGTACCTAAAGCCCAGCCAATCAC CTAAAGTCAACCTTCGGTTGACCTTGAGGGTTCCCTAAGGGTTGGGGATGACCCTTGGGTTTGTCTTTGGGTG- TTACCTTGAGTGTCTCTCTGTGTCCCT SEQ ID NO: 2-Enterobacteria phage SP6 TCTCTCGGCCTCGGCCTCGCCGGGATGTCCCCATAGGGTGCCTGTGGGCGCTAGGGCGGCCTGTGGAGGCCTG- AGAGAAGCTCTTAGTGTGGGCCAAAGGGTAACCTGA GGCCTGCCGGAGCGAGCGATAGGGACGCGTGTAGGCCGCTTGACAGCGTGTGTGGGCGTGGGCTATCTGTTCG- TTTGCTCCGCTTACGCTACGCTTCACTCACGGCCTTG TGTACCTTAGGGTCTTCCTTATCGTGTACCTTGGGACAGTCTTAGTAACTACCTTAGTCACTTCCTTAGTAGC- TTCCTTAGTGAGTAGCTTAGTGGCTATCTATTGCTGTCTT AGTGTTACCTTAGTGATTGCATAGCTACGCTATAAGATGCGAATAGGTCGCGGTCGGTAGACCGCTAAAGAAA- GAGAAGAACAATAAGATGCAGTAGGAGGGACACCA GAATCCTAGCCAGCCTAACCTATCCTAGCTCTGTATCTATTGCTTTTCCTTAGTCCAACACGTTAGACAACCT- ATGATTATCTTAGTAGCTGTGACATGTATCACATAAATA ATCTATCTTAGTGAAACTTAGTGTTGACACAGGCAGTAGTCGGTAGTACATTACAGTCATCGGGAGGCAACCC- AGCCGAACGATAGGTAGCTTTGGCTGCCTTGCTCTTT AACAATATGGCTAGTGTCTTGATAGGCTAACTAACTGAGGTTACTATCATGCTCAAAGAGACTCAAATCAAGC- ACGAAAACGGAAAGTATTGGGTGTTAGAGGTTAAGA AAGGTATGTATCAGGTGATGATATCTGGCTTAACTCACTCAACTTGTGATAGTGCTTACAACGATCTTAGCTT- AGCTATTTATCGGTGCGATTATCTGGCTAAACGAGCAT AAGGTAAGGCTGGCGTAGGCTGGCCTATCAAGGCACTATCCTTGCTCTTTAACAATCTGCTTAGTGTAACCTA-

TGTAAGCCGTGGTATTACTTATTAACTTAATGAGGTGA TACTATGTACGATGAACTGTATGAAGCTTACTTTAACTCTCTGGATGAAGGAGAAGAGGTACTATCCTTTGCT- GATTTTGTAGAGGCTAGGGGAGGTGCTGAATGATGAC CTTGAATCTTAGAGAAGCTAGCGCGGTCTTTACTATGTTATGTTGGATGATACGTAACAACGAAATGATGACC- GATGACGAGCTAGCGCTTTACCACCGCTTTCGTAATG AGGGCTGGGAAGATACAGTGAACAATGTGCGCGACATACTGAAGGAGATAATCCATGTTTAAGCACACGATAT- ACACGCAATGCTGCAATTCAGTGGGCATTATGCGTT GGTGGGATGAGTCTAGTGTTAAGTGCTACAATTTGAATGATGATAGCACTATGTATGAGGTTACTCTCATTAA- AAGATATAACCACGACACGCTGTTATGGATTCTATCT GAATGGGAACTAACCTATGAAGATGTGATTACAGAAGAAATTTAAATTAACCATTGACTACCACGGCTTACAT- AGGTTACATTAAGCACCAACAAGAAGTAACGATCTT TAACAATCTGGATTGAAGCCGATTAGATAGAGGTTAACACATAGGAGGTTTACGAGCCTCCTAGATGGTAACT- TACTAACTAAGAGGAAATAGAAATGGCAATGTCTAA CATGACTTACAGCGACGTTTACAACCACGCTTACGGATTGCTGAAAGAATACATTCGCTACGATGATGTACGC- AACGAGGACGACCTGAGCGATAAAATCCACGAGGCC GCTGGTAATGCTGTTCCGCACTGGTACGCTGACATCTTTAGCGTAATGGCTAGTGACGGTATTGACTTGGAGT- TCGACGACTCTGGTCTGATGCCTGACACTAAGGACGT AACGTACATCCTTCAAGCTCGCATCCATGAACAACTCACGATTGACCTTTACGGGGACGCTGAAGACCTGCTT- AATGAGTATTTAGAAGAGATTGAAGCTGAAGAAGAC GAAGAAGAGGACGAATAAATGAACGGCAAACAATATACCTTTCAATTTTCTGATGGTATTACCTTGAAATGTT- CTCTAAGGTTCGCCATGATGCGAGAGGAAACATTAG GAACTAGTTATAAACTAGTTATGTGACACTATAAGATGATTAACAGGGTATTCTTGCGAGAGTACCCGATTAA- TCTAATTTGATGAGGCGATTATGAGTAAAGTAACAAA CATTTTAGTCTCTATTGTAATCCTGTTAGTTGTGCTGTGGTCTGCAATAGGTTCTAACTTCCAGTGGTTTAAC- ACCTGCTATGAAGGAGATTTACACACTAAGCACTTACAG TTTAATGGTGTTACAATATATTCCACCTTTGAAAACCATAAAGATAACCCTTTTCATAAGTAATAGCCTATAG- TGTCATTCGTGGCACTATGTGAAATTACTTAATAACAT ATGGAGAACATACCATGACTACTGAATACACCATTGTAACTCTTCGTGAAGCTGCAACCGCTGAAATCAAAGC- ACATTTAGACACCATCGGCGCTTCCTATATCAAGATT GGTACTTGCTTAAACGAGCTACGCGCTGACTTTGACGGTCAAAAGGAGTTTTTAGCTTATGTTGAGGCTGAAT- TCTCAATTAAGAAAGCACAATGCTATCACCTGATGAA TGTAGCGCGTGTTTTCGGTGAAGATGAGCGCTTTAAAGGTGTGGCGATGCGTGTAATGTTGGCGCTTATTCCG- GTAGCTGATGAAGCCTCCGTAATGGGTAAGGCCGCAG AACTGGCGGCTAATGGTGAGCTGGATACTAAGGCCGTAAATAAACTGCTTGGAAAGCCTCAGGCCACGCCTAA- ATCTGAACCTAAGCAATCACATGGCGACGAAGAGAA AACGCCTGAGAGCGCCGCACAGGGAGCGCCTCAGCCATTGCAGTCAGTACCTGAGGAAGATAAAGCGCCTTGG- GATGAAGACACCACGCAAACTGTGAAAGATGATTC ACAGAAAGCACCTGAGACAGCCGCGCCGCGCCTGGATAACGCTGAGACCGCAGACAGTGCGGCTATGGCTAGC- CTGTTAGACCAGATTAGCAAGCTGACAGAACAACT AACATTAGCTAACAACCGCATCGCGGAGTTAACAAGCGCTCGTGAATCCAAGAAAGCAAGCGCTCCAATGCTC- CCACAGTTTAAATCTTCATGTTTCTATGCTCGCTTAG GTCTGAGCGCGGAGGAAGCAACCAAGAAAACAGCAGTTAACAAGGCTAAGCGTGAACTTGTTAAGCTAGGGTA- TGGTGAAGGTCATGAAGCGTGGGCCTTGATTAGCG AAGCAGTAGAATCCTTAACTAAATAAAGTTGACTTATAGAGCGTCATTAAGTAAGATGGCGCTCAATTAAGTT- TTCTAGTACCGCATGAGGATACAAGATGCAAGATTTA CACGCTATCCAGCTTCAATTAGAAGAAGAGATGTTTAATGGTGGCATTCGTCGCTTCGAAGCAGATCAACAAC- GCCAGATTGCAGCAGGTAGCGAGAGCGACACAGCAT GGAACCGCCGCCTGTTGTCAGAACTTATTGCACCTATGGCTGAAGGCATTCAGGCTTATAAAGAAGAGTACGA- AGGTAAGAAAGGTCGTGCACCTCGCGCATTGGCTTTC TTACAATGTGTAGAAAATGAAGTTGCAGCATACATCACTATGAAAGTTGTTATGGATATGCTGAATACGGATG- CTACCCTTCAGGCTATTGCAATGAGTGTAGCAGAACG CATTGAAGACCAAGTGCGCTTTTCTAAGCTAGAAGGTCACGCCGCTAAATACTTTGAGAAGGTTAAGAAGTCA- CTCAAGGCTAGCCGTACTAAGTCATATCGTCACGCTC ATAACGTAGCTGTAGTTGCTGAAAAATCAGTTGCAGAAAAGGACGCGGACTTTGACCGTTGGGAGGCGTGGCC- AAAAGAAACTCAATTGCAGATTGGTACTACCTTGCT TGAAATCTTAGAAGGTAGCGTTTTCTATAATGGTGAACCTGTATTTATGCGTGCTATGCGCACTTATGGCGGA- AAGACTATTTACTACTTACAAACTTCTGAAAGTGTAGG CCAGTGGATTAGCGCATTCAAAGAGCACGTAGCGCAATTAAGCCCAGCTTATGCCCCTTGCGTAATCCCTCCT- CGTCCTTGGAGAACTCCATTTAATGGAGGGTTCCATA CTGAGAAGGTAGCTAGCCGTATCCGTCTTGTAAAAGGTAACCGTGAGCATGTACGCAAGTTGACTCAAAAGCA- AATGCCAAAGGTTTATAAGGCTATCAACGCATTACA AAATACACAATGGCAAATCAACAAGGATGTATTAGCAGTTATTGAAGAAGTAATCCGCTTAGACCTTGGTTAT- GGTGTACCTTCCTTCAAGCCACTGATTGACAAGGAGA ACAAGCCAGCTAACCCGGTACCTGTTGAATTCCAACACCTGCGCGGTCGTGAACTGAAAGAGATGCTATCACC- TGAGCAGTGGCAACAATTCATTAACTGGAAAGGCGA ATGCGCGCGCCTATATACCGCAGAAACTAAGCGCGGTTCAAAGTCCGCCGCCGTTGTTCGCATGGTAGGACAG- GCCCGTAAATATAGCGCCTTTGAATCCATTTACTTCG TGTACGCAATGGATAGCCGCAGCCGTGTCTATGTGCAATCTAGCACGCTCTCTCCGCAGTCTAACGACTTAGG- TAAGGCATTACTCCGCTTTACCGAGGGACGCCCTGTG AATGGCGTAGAAGCGCTTAAATGGTTCTGCATCAATGGTGCTAACCTTTGGGGATGGGACAAGAAAACTTTTG- ATGTGCGCGTGTCTAACGTATTAGATGAGGAATTCCA AGATATGTGTCGAGACATCGCCGCAGACCCTCTCACATTCACCCAATGGGCTAAAGCTGATGCACCTTATGAA- TTCCTCGCTTGGTGCTTTGAGTATGCTCAATACCTTGA TTTGGTGGATGAAGGAAGGGCCGACGAATTCCGCACTCACCTACCAGTACATCAGGACGGGTCTTGTTCAGGC- ATTCAGCACTATAGTGCTATGCTTCGCGACGAAGTAG GGGCCAAAGCTGTTAACCTGAAACCCTCCGATGCACCGCAGGATATCTATGGGGCGGTGGCGCAAGTGGTTAT- CAAGAAGAATGCGCTATATATGGATGCGGACGATGC AACCACGTTTACTTCTGGTAGCGTCACGCTGTCCGGTACAGAACTGCGAGCAATGGCTAGCGCATGGGATAGT- ATTGGTATTACCCGTAGCTTAACCAAAAAGCCCGTGA TGACCTTGCCATATGGTTCTACTCGCTTAACTTGCCGTGAATCTGTGATTGATTACATCGTAGACTTAGAGGA- AAAAGAGGCGCAGAAGGCAGTAGCAGAAGGGCGGAC GGCAAACAAGGTACATCCTTTTGAAGACGATCGTCAAGATTACTTGACTCCGGGCGCAGCTTACAACTACATG- ACGGCACTAATCTGGCCTTCTATTTCTGAAGTAGTTA AGGCACCGATAGTAGCTATGAAGATGATACGCCAGCTTGCACGCTTTGCAGCGAAACGTAATGAAGGCCTGAT- GTACACCCTGCCTACTGGCTTCATCTTAGAACAGAA GATCATGGCAACCGAGATGCTACGCGTGCGTACCTGTCTGATGGGTGATATCAAGATGTCCCTTCAGGTTGAA- ACGGATATCGTAGATGAAGCCGCTATGATGGGAGCA GCAGCACCTAATTTCGTACACGGTCATGACGCAAGTCACCTTATCCTTACCGTATGTGAATTGGTAGACAAGG- GCGTAACTAGTATCGCTGTAATCCACGACTCTTTTGGT ACTCATGCAGACAACACCCTCACTCTTAGAGTGGCACTTAAAGGGCAGATGGTTGCAATGTATATTGATGGTA- ATGCGCTTCAGAAACTACTGGAGGAGCATGAAGAGC GCTGGATGGTTGATACAGGTATCGAAGTACCTGAGCAAGGGGAGTTCGACCTTAACGAAATCATGGATTCTGA- ATACGTATTTGCCTAATAGAACAATAAATATACAGG TCAGCCTTCGGGCTGGCCTTTTCTTTTAACTATTACCTGTAACATTTAATTAACAAGTCCAACGTGTTGGACA- CGATGCGGATTTAAGGGACACTATAGGACTACCCGTCG GAGACGGAAAGTAATAGGTAATAATAGGAAGTAGTAGGTAAGTAAGGTAATTATAGGTTACTTAGGTTACTCC- TTCCTATTACCTCCTTCTTAATAGGAAGGGCAGACAC TAGGTTGTCTAACGTGTTGGACAGAACTTATTTACGTGACACTATTGAACTAATCAACATTCAATTCATTGGA- GAATTAATCATGCGTAACTTTGAGAAACTGACCCGTAA GCCTGCTAATCGTTTTGGCATGGAGGAAGGGAAGACAGGCGCCAAGCGTAACAAGCCTACCCGTGACCGTGTA- TCTAAGCGTGCAGTGTGGGAGTACTAAGTTATGGCT ATTATTAACAATATTCCGTGCCCTGCCTGTCAAAAGAATGGACATGATAAATCTGGCAATCATCTTATGATAT- TTGATGATGGCGCTGGTTACTGCAATCGTGGACACTTC CATGATAGTGGCAAGCCTTACTACCATAAGCCGGAAGGTGGCATCGAAATCACCGAGCTACCCATCACTGGCA- ATATCAAATATACACCTTCTCAATTCAAAGAAATGG AGAAGGAAGGGAAGATAAGTGACCCTAAACTTCGTGCTATCGCCTTGGGTGGTATGCGTATGAAAGATCGTTG- GGAGGTGATGAATGCGGAAGAAAGGGCGGAGCAAG AATCTGAATGGCAGCTTGACGTTGAGTGGTTCCTTGAACTTAAAAGGAAGAACCTTGTATCACGACACATTCG- CGGAGACATTTGTGCGCTTTATGACGTCCGAGTAGGT CATGATGGAGAAGGGAAGGTTAATAGGCACTACTACCCTCGCTTCGAAGGTGGCAAACTTGTGGGAGCTAAGT- GCCGGACGCTACCTAAAGATTTCAAGTTTGGACATC TAGGTAAACTGTTTGGCAACCAAGACATGTTCGGTATGAATACCATGTCTAACGTGTTGGACAAGGGACGAAG- GAAAGACACCCTGCTTATCGTGGGAGGTGAACTGGA TGCACTAGCAGCACAGCAGATGCTTCTGGATTCTGCCAAAGGTACGAAGTGGGAAGGTCAGCCTTACCATGTG- TGGTCTATCAACAAGGGTGAGGCTTGCCTTGAAGAG ATAGTACAGAACCGTGAGCACATCTCTCAGTTCAAGAAGATTATGTGGGGCTTCGACGGTGATGAAATAGGGC- AGAAGCTTAACCAACAAGCGGCCCGCCTGTTCCCCG GCAAGTCTTATATCATTGAGTACCCTGCGGGCTGCAAGGATGCTAACAAGGCATTGATGGCTGGCAAATCCAA- GGAGTTCGTAGATGCATGGTTCAATGCCAAGTCATCA GATGAGGTTTTCGGTAGCCAGATTAAATCCATCGCCTCTCAAAGGGACAAGCTGAAGGCTGCACGCCCTGAAC- CGGGATTATCTTGGCCTTGGCCTAGGCTGAACAAGAT AACCCTTGGCATCCGTAAGCATCAGCTAATCATCGTCGGCGCTGGTTCTGGTGTAGGTAAGACTGAGTTCCTC- CGCGAAGTAGTGAAGCACCTCATTGAAGAACATGGAG AGTCGGTAGGTATTATCTCCACTGAAGACCCTATGGTTAAGGTCTCCCGCGCATTCATTGGTAAATGGATTGA- TAAGCGTATTGAACTACCTCCAACCAATGACCCAAGA GAAGATGGATACCGTGAGGTCTTTGATTATACCGAAGAGGAAGCCAACGCTGCCATTGACTACGTTGCTGACA- CTGGTAAGCTTTTTGTAGCTGACCTTGAAGGTGACTA TTCTATGGAGAAGGTAGAGCAGACGTGCCTTGAGTTTGAGGCAATGGGTATTTCTAACATCATCATTGATAAC- TTAACAGGAATTAAATTAGATGAACGAAATTTTGGTG GTAAAGTTGGTGCGCTTGATGAGTGCGTCAAAAGGATTGGCACTATCAAAGACCGACATCCGGTTACTATCTT- CCTTGTCTCGCACCTTACACGTCCTTCAGGACAACGT ACCTCACACGAAGAAGGTGGCGAGGTTATCCTTTCTGACTTCCGAGGCTCAGGGGCTATCGGATTCTGGGCTT- CTTACGCCTTGGGGATTGAGCGTAATACAAGAGCTGA AACGCTTGATGAAAGGACTACCACGTACATCTCATGTGTCAAAGATCGAGACCAAGGCATCTACACTGGTACT- AAAGTGATGCTCAAAGGGGATGTTAGTACCGGCAGA TTAATGGAACCACAATCACGTACTAAATCATTTGATACAGGTGCTCCAAAAGAGCAAGCTGTGCCTGATGAAT- TAGGTGACACTATAGAAGAGAACACACAGGAGTTTA ATGGATGATTTAGGTTTTGGTTGTTCGCTACCGTACTACTTGTTATTAACATAGACAAGGTTGCTATGTTATT- CAAATAGTGTACTTATCAGGGTTTGTCTAACATGTTGGA CAAACTCTTATTAAGTACATTAACTAACTGGAGATTATTATGTGTAAATTGCACCTCAACAAATCAGATTGTG- TGCGTAACATTAACAAGAGATCTATCCGCTTTCGCTGG GAGGGTGTAGTGTTTGATGTAGATGAGAGATACTACCATGTAGTGTATGGTAATGGATTACGTCAAACTTATC- TGAAGGCTCTGGCGCATCATTACCTTGAACCGATTGA ACCAACTAAGAGTAACTGCACCTGTGTACACGATGATCTGTGTGATCGCTGTGCTCGTCAAGTTAATAAGGCA- TTGACAATCATGGAGCGTTACGGTGCAGGCCACAAGG CAATCTCTGAGGCTGCGTGGACTGTACTCATGTTTGAACGCCCTAATGGTCGTAAGGTGCTGAATCGTGAGCG- GCGTAATGTAATCACAGGTCAAGACTTTCGCATCTTA GAGGAGGCTATGTGTAATCCTGGTATTGCTATACGTTATGAGGATGTAGACCATGCTATATCTGAAGGTATCG- GTAATCGTTTGGAATTGAATAAGCATTTTGATCAGGT ATTACGTGACACTATAGGTGGGCGCAAAGGTTTTACCTTTGAGCGCGGGCATGTTACATTTAACCCTATCGTT- ACGGAGGAAACCTATGTCACGCAATGACAGTAAGTAC AGCCTGAAGTTCCTTGAGCAGCATGAAGAACTTGCAGCCAAGGTAACTAACCAAGCATTCCTGTTTGCACAAC- TAACGCTGGCTGAAGCTAAGAAGAACAGCCTTACGC GTGAGCAGATTATCAAGGAAGGAACCAAGCGCAGTTAATAAGTCGTGACTTGTCTAACATGTTGGACAGGTCA- CTCTCATATTAATTGGAGATACATAAATGACTAAAG TAACTAAGTTAACCGAACACCTGATTAAACTAAGTGAAGAACTAAAGAACAGCGAAGTTAGGCTTGAGTATTA- CTTCATTGACCCAAGGGAAGATGATCGTGAAACACC TGACTACAAGTTTGAAACGGAGTTAATGTATGAAAACTATTAATTGGGCGAAGGAAGCAGAAGGACGTATCCT- AGTAATGGATGCGGAGGCTAAAGGCTTACTTGATGC AATCCGATATGGAAAAGGTAACGATGACGTGCATATAATTTGCTGCATGGACTTGCTCACCACTGAAGAGTTT- CTCTTCTTCAACCCATATGACCGTCGTGACCCTAACG CAAGGGAGCACCTGAAGGAGTGGGATGGTCATCAGGACGGTGACCTTGAAGATGGTGTGAGATTCCTCAAGCA- CTGTGAAGCTATCGTGTCACAGAACTTCCTCGGCTA TGACGGCTTGCTTTTTGAGAAGGCATTCCCCGATATATGGAAAGGCTATAACTACACGGAGAAGCGCGGCAAA- GGCCGTCTGCGGGCCGATCTGTGCCCGGTTAAGGTA ATGGATACCCTTGTCATGTCAAGGCTCCTGAACCCGGATAGGCGACTCCCTCCGCAGGCATACGCTAAGGGTA- TGGGTAACGTTGCACCTCACTCTATTGAGGCACACGG TATCCGTATAGGTCGCTATAAGCCTGAGAACGAGGACTGGTCTAAGCTGACAGACCACATGGTGCACCGAGTA- CGTGAGGATGTGGCGATCGGTCGTGACCTGTTCCTGT GGCTGTACAACGGCGAGTGGATGGAGCACAAGCGGCGTGGCGTCAATCCAAGGACTGGTCTTGGCATTGAGAC- AGCCTTCCACATGGAGTCCATTGTAGCACTGGAGAT GTCTCGTCAAGCGGAGCGCGGCTTCCGGCTGGATATAGACAAGGCACTGGCACGATGCGAGGAGCTTGACCAG- AAGATTGACGAGACTGTTGCAGCCTTCCGGCCTCAC ATGCCAATGCGCATCAAGTCTAAGCCTTTCAAACCTCAAGAGAAACAGGAGCAAGTAGATGCGGCAAACTCAT- TTAGTTTACAGAATCATACTGGCGTTACACTTGGAG CCGATGCTTTCATTCATGCCGAGCGGCGCTCCGATAGAAAGACTGTATGGTCAGTCACTACTAAGTCAGGTGA- TTGGTCAGCTACTGTCAAGAAAGACTTCCCTCACATC CGAGGAAACATCAATGATACTCCGAGTATTAAACACATCGGGCCATATACACCTGTCACCTTCGAAGATATCC- CGCTTGGTAACCGAGACACAGTTAAGCAGGTTCTGTA TGACTTTGGGTGGAGGGGAGTTGAGTTCAACGACACTGAGCAATCTTATCTGGACGAGCATGGAGTGTTGCCT- AAGCCGTGGAGTGGAAAGATAAATGAGAAGTCCCTT ACTTTATGGCAGGAAAGGGCTGCACGTGAAGGTAAGTCAGTACCTGATTGGTGCTTGGGTATCGCTGCATGGT- ACATACTCGTATCCCGTCGTGGTCAGATCCTCAACCG TGGTGATGTTGAAACCTTCGATTCAACGGGGCGTTGGCCCTCGCAAGCTGGTGTACGAAAGTGTCGCGGCCTC- GTACCTGTAGCCTTTAACAAGGAGCTAGGTATCAATG CACAGGCATACTACGAAACATATGGCTACTGGCCTACGTCCGACAAGGATGATGGAGAGTGGCGTGTTCCCGC- TGTTGCTATTTCTATTGGCACTTCTACGTTCCGTATGC GTCACAGGAATGTGGTTAACATCCCCGCTCGCGGTCTTTACCCTCTTCGTGATTTATTTATAGCTGGTAAAGG- TAAGATGATTCTTGGTTGTGATGGTGCAGGACTGGAGT TGCGTGTGTTATCACACTTCATGAATGACCCTGAATACCAAGAGATTGTACTGCATGGTGACATCCATACACA- CAACCAACTTAAGGCTGGTTTACCTAAGCGTGACATG GCGAAGACTTTTATCTACGCATTCTTGTATGGCTCTGGTATTGCCAACCTTGCCGCTGTATGTGGTGTAACTG- AAGATGAGATGAAGGAGGTTGTTGCACGGTTCGAGATC GAACTACCATCACTGGCTCGTCTTCGTGAGAATGTCATCGCTGCTGGTAATAAGTTTGGATACCTGCAAGCAC- CTGATGGTCATTGGGGGCGCATCCGTATGAGTGGTGG TGAGCTTAAAGAACACACCATGCTCAACGTATTACTTCAGATGACAGGCTCCTTGTGTATGAAATATGCCTTG- GTTAAAGCCTTTGCAGTCATGCGCCGTGAAGGTGTTG CACTGGATAACCTGGGGAATCCGTGTGGCGTGGCTAACGTACACGATGAAATCCAGATGGAAGTGCCAGAAGA- GGAGGTGTTATACCTTGACTATGAATTACCTTTCAC GTTGGAAGGTTTCGAATCTGAGAAGCAAGCTATCAAAGCTGTGTTCGACCCTGAAGAGAAGCGCGTACATGTG- GATTCCGAAGGGCGCATGTGGTCTGCTGCTAACTTG GTTGAAGTGGATACTGCTGCTGGCGTGCTGCGTTGTCAGCGTCGCTACCACAGGGCTGGTCATATTATCGCTG- ACGCCATGACATGGGCTGGTAAGTACCTGAATATGCG CTGCCCTATGGCTGGCGAGTACAAAATAGGTGCAAGCTGGAAGGAGACACACTAATGCAAACTGCTCTTATTA- TTCTTGGAGTCATATTATTTATGGTAGTGTTCTGGGC CTTCTCTGGTATTGACCCAGATTACGATGGTAACTACGACTGAGTTATACTCAAGGTCACTTACGAGTGGCCT- TTATGAATAACTTAACTGGAGATTATTATGATTAAATA TTGCTTATCAATTAACTAAAGACCGTAAGATAGGTATTGAGGTTAAAGCCTGGGACGCGGGACACATCTCTGT- AGTTATAGAGTGCCGCCAAGACAATGGTATGCTGTTA AGAAGCTACCGTTGCTTCACCAAATTACGCTGCAAAGATTTAACTGAAGAATTATTCTTACGTTGTATTGTTG- AATCTATTAAACTTATTAGACCTTACGCTAAGCAAGTT GTAGGTAATGTCACAGTGGTAAATTGATTTAGGTGACACTATAGGAGGAAGACCTAGGTAATCTAGGTTTATA- ATGTAGTATAGGTAATTAAGTAAATATAGGAGATAT AAACATGTCAATGGTAACTACTCTGGTATTCGTGGCTCAATACTTTCGTGGTCTGGCTAATAAGTTCAAGTAC- AAAGCTATTGAAGCTATTGAGGACCGCATCGAAGCAG TACAGGCAGAACAAGTTGAAGTTGAAGAACATCGTAGTTCTCAAATGATTGACTGCCATAATCGCTATTACGC- ATCTCGTGATGACCTTAATGCACGACAAGTCAAAGAG GTCGAAGAGATGATGGCACGTCACCAGCAAGAGCGTGACAACCTGAAGGCTGACTTTGAAGAGCGCAAGGCAT- CCATTGCCCTTGTACATCAAGCTGCATCTGACAGCC TGAAGAAAGAGATTGTTATGCTGGAAGTGGAGTTAGACAATCTGACCAAATAATTAGGTGACACTATAGAACA- ATAGGACGTGGGTTTGTCGGAGACAGTAAATCCAAG GTGCTCAGTGAGCGTAAAGCCTAAGCACGTCCTATGATTGTAAAGTGTTGAACCTCTTGTGCATCTTGCACAA- CCCGATACAGTATCGGGCTTTCTAGTGAGTACATGCTT GTGCTCAGTACAAAGCTAACAAACAACAGGAGGAATAAATTAATGGCTCGTAATTTTGATTTTGGTGCTGAGG- TTGCTGCTGCTACTGGTGGTGTGTTTAAGAATCCAGA AGTTGGTGATCACGAGGCAGTTATCTCTGGAATCATTCACGTTGGTTCCTTCCAAGACATCTTTAAGAAAGGT- AACACTACCGAGGTGAAGAAGCCTGCTAACTTCGTTC TTGTTAAGGTTATCCTGATGGGTGACGATGACAAGAACGAGGATGGTTCTCGTATGGAACAGTGGATGGCTGT- GCCGCTCAAGTCTGGTGACAAGGCGACGCTGACCAA GTTCCTGAATGCAGTTGACCCTAAAGAATTACTAGGTGGTTTCGATGACTTCATCGGCGAGTGCATGACTGTG- AGCATGGTTGGCGATGAGAAAGGTGGCAAGAATGAT GACGGCACCTTCAAGTACGTTAACTGGAAAGGCTTCGGTGGTATGCCGGATAAATTGAAGAAGCTGGTACTGG- CTCAGGTAGAGGATGAAGGTCTGGAAATGACTGGTC ACATCACCTTTGACAAGCTGACCAAAGATATCATCGACTCTATTCCTGCACACCTTGTACGTCAGTACCTGCT- GAACGAGACGCCGCGTGGTAAGAACCTGTCAGTAGTT GGTTCTCATGTAGAGGGTATCATTGCCGAAGCACGCGCAGCAGACCCTGAGTGGAAGAAGGCCAAGAAGAAAG- ACAATGAGGCCACCCCTGAAGACCGCAAGACGCTG GACACTGGCGCTGCTGTTCCGCAGGAAGTACCGGAAGCGCAGAATGCCCCGGCACCTGCTATGGATGAAGATG- CTGAATATTAATCAAGGAGGTTTAATGAAAGTAGAA GCAGTAACCCTACACTTCAAGCCCGGCGTAACGTCGCTGGGCGGCACGCAGTTCATTTCTTTTAGCGAGGGCA- AGGCCTACCAAGACCTGCACTATATTACCCGTGAGGG GCAGCACGTCGTGAATTACAGCGACCCTGTGACAGGCAAACGTCACGGCATTGGATTCCCTATGACGGACATC- CGTCAGACCAATACGATTTTGTAAGTCTAACGCGTTG GACAAATCTGTGTCTCTTATTTAGGGGACACTATAGAAGAGAGAATTTTAATCGGCGATAATGCCACAATTAA- CAGAAGGAGAATTTAAATATGTTCACTATCGAAACTA TCGTAAACCGTGTTGTTAAAGGCGCTACCTTGGTATCCGTTGAGTCTTTCATTATCGTCGATGAAGCTGGCTC- GCTGGTAGCTGGCACCAAAGCATACGACACCCGCGAA GAAGCTCAAGCTAAGATTGACAGCATGGGTAACTTTGCTACTGGCCTGGAGTTTGCTCGTGCTTGCTTCCCTG- AGCAGGCTGACAAAGCACAGATTGGTAAGGCTAACAT TGTAGCTGAATATCTGGATTGGATTGCTGCTGGTAAGCCAGTGAAAGAAGTTAAGTCTGCTGAAGAAGCTGAA- GCTCCGGCAGTGGAAGCTGCACCGGAAGCTCCGGTT

AGCGAAGAAGAAGAGTTTTAATTGATGCCCTGTCTGCCTTAGTGTAGGCAGGGTCTTTTGCGTAATAGTTATT- GGAGAATGAATTATGCCGACTATTAGATCTCGTTTAGT AGCAGATTATGTGTATGGTCGTGATGTCAAAATGATGAAAGATTACCTCAAAGTTATTATCTTGCTTGATGGG- GAGTTGTTTCATACTAAAACCTTCACCCTTCCTGAGTT ATTTGACTTAGGATATTGGGGTTATACCTATCAGGCCATAGCAAATAAGGTGCTACTCGATGTATTAAAGGAG- TGGCCTACATGCGACCAAACTTCAACTTCGGAGCTAC AGTATCGGAAGACAATAATCTCATCCTGTGGCCGACTGAAGGTAAGAGAATCGCTCTCATAGATGGAGATATG- ATTCCATACATCATTGGTTATACTATCAATGAGATGA CACTTGTCCGAGCGATGACCCGCGTTAAGTCAGGGCAAGTAGAGCGCATCGAAGATACACCTGAGTGTAAGCA- AGCTTGCGACCGTGTAAACTCTATGCTTAACTCTTGG GTGTATGGTGCTGAATGTGATGCCGCACGCATCTTCCTCACCAAGTCAGATACTAACTTCCGCCTACGCTTGG- CTTTCACGAAACCATACAAAGGTACACGAAAGGCAGA CAAGCCTCCTTTCTTCTATGAGATGCGACAACACCTGATAAGTGTGCATGGTGCAGAACTGGCAGATGGGGAG- GAAGCAGATGACTTGATGAGTATCGCACAATGGGAT AGCCACAACCGATTCTTGCAAGAAGTAGGTAACGAGTTCTCAATAGGAAGCCCTGAGCATAAGGTGTTCTCCG- ATACCGTTATTGTATCTGCGGATAAAGACCTGATGAT AGTACCGGGGTGGCACTTGCAGCCGGGAAGTGAAATGAAGTGGGTTAAACCTATGGGTTGGCTTGACCTTCGT- CGTAAGAATAACGGGCAGGTCAAAGACCTTAAAGGT GCAGGACTAAAGTTCTTCTATGCACAAATGATTATAGGTGACGACATAGATAACTATGCAGGCATCCCAGGAC- GTGGGGCCAAGTACGCTTATGACCTCCTTGATAGTTG CAAGACTGAGAAGGAACTCTATATGGCTGTGCTTGGTGCCTACAAGTCTAAGTTTGGAGAAGGGCCAGTCAAG- CTCAAGAACCATAGAGGAACCTACCGCATCGGCAAG GCTTTTGATCTGATGTTAGAATGTGGCCGCTTGGCTCATATGGCACAATTCAAAGGTGACATCTGGCGTGCGG- ATAAGAATCCAATTGTGTGGGGAGATGATGATTCATG GCAATCAGATTGAAGGCTTCGGAGGTAGCTGACTACAAGAAAGAGCTACTAGAGAAGCAGAAATGGAAGTGCC- CTTTATGTGGCGGCAGCCTCAAGGCTGTCACTGCAA TTAACCGTGTACTTGACCATGACCATGAGACAGGCTTCTGTCGTGCAGTGGTTTGTCGTGGCTGCAATGGTGC- GGAGGGTAAGATCTTAGGTGTTATTTCTGGTTATGGTA AGGCAGGTAACAATCGCTACTTCCAACTGAAGTGGCTGGAGAACTTGTATACATACTGGAAGTTACATCAAAC- ACCTCAGACGGATAAGTTGTATCATAAGCATAAGAC TGAGGCGGAGAAGCGCGAGGCTCGCAATCGCAAGGCTCGCTTGGCATACGCAAGAAAGAAGGAGGGTAAAGTT- GGGTAAGCTACGCTCACTGTATAAGGACTCCGAGG TACTTGATGCAATAGAGCAGGCTACCGACGAGAAAGGTAATGTTAATTATAACGAGATGGCTCGCGTACTTTC- TGCGCATCCTGTCGGCAAGAAGATTACACGGCAGCTT GCTCGTTACTGGCATGGTCAATTCATGCATACCAAGAAGAACGGTGACTACTACCAGACTCTTTCTCAGGAGG- ATAGGCGACTCAAAGAAGCACGTAAGCTCAGGACTC CTGACCGCTATGAGGATCTGGCTATTGTACCATTGCCTGACTCGCCTCATAGAAGTGTACTGGTGATCCCTGA- TACCCATGCACCTTATGAACACCCAGATACCTTGGAGT TCTTGGCAGCAGTGGCGGCACGCTTCCGTCCTGATACGGTGGTTCACTTAGGAGATGAGGCAGACAAACATGC- CTTGTCATTCCACGATAGTGACCCTAACCTTGACTCC GCTGGTGTGGAGTTGGAGAAGGCACGTGCCTTCATGCACAAGCTGCACCGGATGTTCCCGGTCATGCGCCTGT- GCCACTCCAATCATGGTTCTATGCACTTCCGCAAGGC AAGCGCCAAGGGCATCCCTGTCCAATATCTGCGCACTTACCGAGAAGTCTTCTTCCCGCATGGTGGCGGCGAC- CAATGGGATTGGCAACACACTCATGTCCTGGAGTTAC CTAACGGGGAGCAGGTTGCATTCAAGCATCAACCAGCAGGTTCTGTGTTAGCAGATGCGGCACATGAGCGAAT- GAATCTGGTGTGCGGCCACTTGCATGGTAAGATGTC AGTGGAGTATGCACGTAACACACATGAGCAATATTGGGCTGTGCATGGTGGCTGTCTTATTGACGAGTCGTCT- CGCGCATTTGCTTATGGCCGTGAGTCCAAGTATAAGC CAGCATTAGGTTGTGTGGTGATTGTAGAGGGTGTACCTCAGATTGTTCCAATGCAGACCAATGCAGAAGGTCG- TTGGATTGGCAGGATTTAAGTGACACTATAGAACAAA GGGTCAGGTAATACTTATCGGCTGGCATATCCAAATGATATTGCACTGGCCCTTGATTGTATAGTGAATGGAG- GAATTAATTATGTCAGAAATTGATATTGGTAAGTACG TTGTACGCCGTGCAGCTTATCGAGATGCCTTCTGGAATAAACTGTGTGAAAGCTTAAACAAGCAACCAGATGG- GGTGTTCAAAGTGTCCAGTGTAGAACTTAACTACAAC TCTATCATGTTAGAAGGTGTGGAGAAACGCGAATGGTATGCACCTTATTTCCAGGTCGTTGACTCCCTGCAAG- GCGAAGAGTCCAACATGTTGGACAACAACATGGTTAC TAAGCCTAAGCACTATGAGTTCTTCGAGGGTGTCGAGGCAATCACTATCATTGCCCGTAGCATGACCGAGAAG- CAATTTGCTGGTTACTGCATGGGTAATGCATTGAAGT ACCGTCTGCGTGCAGGTAAGAAGTTCAATACTGAGGAAGACCTGAAGAAAGCAGACTACTACAAAGACCTGTT- CCAGAAGCATCGCCATGAATGTATTGATGAGGATCT CTAATGAATATCTTCCAATTCCTAGGTTTACCTGAAGATCATCGTTCCAAACCTGTTATGCTGGTTAAGCACA- GGGATGAAGTGCCAGAAAGCAAACTTACATTCCCGGTT TATGCACAAGTGAAAAGAGATGGAATATTTAGTGCTACAGTTGTGCGTTCTGATGGTACTGTGGGTATCTTTG- GTCGCACTGGCAAAAAGCTGGTTAATGTAGAACAACT GGAAGCGTCTTTTATAGGGTGGCCTGCTGGTGTCTACCTCGGTGAGTTGCAATCTATGGCCGTTGATATCTAC- CTTGAGGCGCTTTCGGGTGTGGTGAATCCAAACAGGAC TGAGCCTCTTGACTTCATAGGACAGCAGATTAAAGATAACCTGTACATTGACTTCTTTGATATGCTGACTATT- AAGGCATTCATCGAAGGGCAGACGGAGGTTACATTCTT AAAGCGATATGAAGCTCTATGTCGCAGATTGAAAGGTTGCCTTCCACCTGAGAATGCAATCCTGACTATCACA- CCTTGCCACACCGAGCAAGAGGTAGAGGCGTTTGCAC AGAAGCACATTGATGCGGGGCGAGAAGGTGCAGTCTTTAAGTTAGACTGTGACTATGAAGCGGGCCACAAGGG- CTTCCGACAGACCAAGATTGTACGCATGGTCTCATA CGACTTAACGTGTATTGGTTGGGAAGAGGGGAAAGGTAAATACAAAGGTAAAGTAGCTAATCTTATATTTAAA- TGGAAGGGTGGCAAGACAATCAAGGCTATGCTTGGC CGTGGCTGGACACATGAAGATGCCACCCGTATGTATCACGATATTAAACACGGTGGTGAACTGAACGTCATCG- GGAAGATATTCGCTATCAAGGCTCTCCAAGAATCTA GCAAGGGAGTCCTGCGACTTCCCAAGGTTGGAGAGTTGCGCCATGACAAGGAGGAGCCTGATGTCTTTTGATT- CAATGAAAGCGACAAAGGCAGTTGAGGTAGCAGAAG CTATCTTTGATATGCTGTCTTGTGGGATTGAAGTCCCTTATACACTTCTGTCTGATGCAGAAGATTTAGGTCT- GTCTGTGGAAGCTATCCGCGAGAAAGTGGAGGAGTTGT ATGGCGACGACCAAGAAGCCGACTATCAATATTGAAGGTTGGGATATGCTGGAGAAAATTATACTTGCTCCAT- CAAGACCTCGACCGGATAAGTCACACGAAGAGTTAG TATGGGATGAAGCCAAGCGCTATATCCTGTCTTGTATCAAGCAGCAGTTTGTGGTGCAGCCATGATAAGGCAG- GCTTGCTTCCTAGATATCCCTGAGATAATTAATCTAG GGAACAGGTATGTAGAAGAGGAAGTCAAGGTAGTTAAGCATCATTCAGCTACATGGGATGCAGATCAAAGCGC- ACATCACCTTTGTGCATCCCTTACCAGCAAGGATTT ATTTCTATGGGTGGCTGTGGAAGATGGTGTTATCATAGGTTTCCTGTGGGCGGCGGCTCACATCATGGCACCT- TGGTCTCCGGCACTTGTGGCTTCTGATCTACTATTCTA CATCATACCAGAAAAGCGAGGGTCTCTTGCTGGTGTGCGCTTGCTCAAAGCTTACAAGTCTTGGGCCAAGGAG- CGCGGCTGCATAGAGGCAAGGTTGTCTATCGCATCTG GTATCAATGAGGAACGTGTGGGGCGGATGTATAGTCGATTAGGGTTTACTCCGTTCGGTACAGTGTATAACTT- GAAGTTTTAAGGAGATAACATGGGTGTAGTTAAGAAG GCATTTCAAGCAGTAGGTCTGGCACAAAAGGCACCTCGCATTGAGGCAGCTAAGGTTCCAGCACAACAACTTG- AGCGGCAGACTGAGGTTAAATCTGAAGACATCCAGA TTGGACAAGAGGATGATGCTGCGGCATCTGCTAAGGGCAAGCGTGGCCTTGTGCGCCCTGTAGCCTCTAGCTT- AGGAGTTTGATATGCAAGACACTATACTTGAGTATGG TGGACAGCGATCGAAGATACCTAAACTATGGGAGAAGTTTTCTAAGAAACGCAGTCCCTACCTTGACAGGGCA- AAGCATTTCGCTAAGTTAACACTCCCATACCTGATGA ACAACAAGGGAGACAATGAGACCTCGCAGAATGGTTGGCAGGGTGTAGGTGCACAAGCTACCAATCACCTAGC- TAACAAGCTGGCACAAGTGCTATTCCCTGCGCAACG ATCATTCTTCCGTGTTGATTTAACAGCAAAAGGTGAGAAGGTATTAGATGACCGAGGGCTGAAGAAAACTCAG- CTAGCAACCATCTTCGCTCGCGTAGAAACCACTGCA ATGAAGGCGCTGGAGCAAAGGCAATTCCGCCCAGCTATAGTTGAGGTGTTCAAGCACTTAATCGTAGCGGGTA- ATTGCCTGTTGTACAAACCAAGCAAAGGTGCGATGA GTGCAGTACCAATGCACCACTACGTAGTCAACCGTGACACTAACGGCGACTTGATGGATGTAATCCTTCTACA- AGAGAAAGCGCTACGTACATTCGACCCAGCAACTCGC ATGGCAATAGAGGTTGGGATGAAAGGTAAGAAGTGCAAAGAGGATGATAACGTCAAACTGTACACTCATGCGC- AATATGCAGGTGAAGGTTTCTGGAAGATTAATCAAT CTGCTGACGACATCCCGGTAGGCAAGGAGAGCCGCATCAAGTCCGAGAAGCTACCATTCATTCCACTTACATG- GAAGCGCAGTTATGGCGAGGATTGGGGCCGTCCCTT GGCTGAGGATTATTCTGGTGACTTGTTTGTTATACAGTTCTTATCTGAGGCCATGGCCCGTGGGGCTGCACTG- ATGGCAGATATCAAGTACCTGATTCGACCCGGTTCACA AACTGATGTTGATCACTTTGTTAACTCAGGTACAGGTGAGGTCATCACAGGTGTTGCGGAAGACATCCACATT- GTTCAGTTGGGTAAGTATGCAGACCTGACACCTATCA GCGCTGTGCTGGAAGTATACACCCGACGCATCGGTGTCATCTTCATGATGGAGACCATGACACGCCGTGACGC- TGAACGTGTTACTGCCGTAGAAATACAACGTGACGC GCTTGAGATTGAGCAGAATATGGGTGGTGTATATTCCCTGTTTGCCATGACCATGCAGACACCTATTGCCATG- TGGGGCTTGCAAGAGGCAGGTGATTCATTCACTAGTG AACTGGTAGACCCTGTGATTGTAACAGGTATTGAAGCACTAGGCCGCATGGCTGAATTGGATAAGCTGGCTAA- CTTTGCACAGTATATGTCCTTACCTCAAACATGGCCT GAACCTGCACAACGTGCAATCCGATGGGGTGATTACATGGATTGGGTGCGTGGTCAGATATCTGCGGAACTCC- CATTCCTCAAGTCTGAGGAGGAGATGCAACAAGAAA TGGCACAGCAAGCACAGGCCCAGCAAGAGGCCATGCTCAACGAAGGTGTGGCTAAGGCCGTACCGGGTGTTAT- TCAACAAGAAATGAAGGAGGGTTAATTAGTGGCCTT TGAATTTGTAGAACCGACCAATGAAACTACCGCTGCTCCGGCTGCTGAAGAGAACAAGGAGGTGACTAATGAT- GTTGCTGGTGTTGACGCTGGTAATACTGGCATTGAC GTACAGAATGGTGCAGATGATCAAGGCAATGAGGACACCGGAGGAGAAGCTGTTGGACAGCCTTCAGGAGAGG- GAGATGGTGAACCGGATGGTAAACCTAAGCCAGAT GGTTCCACGGATGAGGAAGCGCGATACTTCTTCGGTGAACATGAAGTAATCATTGAAGTGCCTGATGATGTGA- CCGAAGCTCTCAAAGAGAAGGGCATCGACGCTATGC AGGTGGCTCGTGAGTTGTATGGTGAAGGTGGTAGATTTGAACTGTCAGAAGAAACCAAGCAGAAACTGTATGA- TGCATTTGGTAAGTTCGCAGTAGATGCCTACCTATCT GGCCTCAAGGCTCAGAACGAAACCTTTTTCCTCCGTGAAGAAACTGCCGCCAAGGAGGCGGAAGCTGCAAACG- CACAGCGCTACACGGATATTGCCAAGGAGGTTGGCG GTGACGAAGGCTGGAGCCGTCTGGAGGAGTGGGCGCTTGATACTCTTTCTGATGAAGAACTGGAAGCATTTAA- TGCAGTGATGCAGTCTGGCAACCAATATCTACAGCA GTACGCTGTGCGCGAGTTAGAAGGTCGCCGTAAGGCTGCACAGGGTGACGATAAACCTAACCTTATTGAACCA- ACGGCTACCGCTGCTGCATCGGAAGATAATGCACCT CTAAGTCGGGAGCAGTACATCCGAGAGATTGCACAGTTAGGCCAGAAGTATGGACGTGACCGCAAAGGGATGG- CTGAAGCACAGGCACGTCTGGATGCACGTCGCCGC GCAGGTATGGCTCGCGGTCTTTAATTGCCTATTTAGGTGACACTATAGAAGGGAGGTAGTCCTCCCTAACCTA- TCAACTTGATTTATAAGGAGATTATAATACATGTCTAC GCCGAACAACTTGACCAACGTTGCCGTTTCCGCTTCCGGGGAAGTAGATAGTCTTCTCATTGAGAAGTTCAAC- GGTAAGGTCAACGAGCAGTACCTGAAGGGCGAAAAC ATCATGTCCTACTTCGACGTGCAGACCGTCACGGGAACCAACACTGTGAGCAACAAATACTTGGGTGAAACCG- AGTTGCAGGTATTAGCACCGGGTCAGTCTCCGGCTGC GACCTCTACTCAGGCCGATAAAAACCAGTTGGTAATCGATGCCACTGTTATTGCCCGTAACACAGTTGCACAC- CTGCACGATGTACAGGGCGACATTGATAGCCTGAAGC CGAAGCTGGCTACCAACCAAGCCAAGCAACTGAAGCGTATGGAAGATGAGATGCTGATTCAGCAGATGATGTT- GGGCGGTATTGCCAACACTCAAGCTAAACGTACTAA CCCGCGTGTTAAGGGTCATGGCTTCTCTATCAACGTAGAGGTTGCAGAAGGTGAAGCGCTGGTCAACCCTCAG- TACGTAATGGCTGCTGTAGAGTTCGCGCTGGAACAGC AGTTAGAGCAGGAAGTGGACATCTCCGATGTGGCTATCCTGATGCCGTGGCGCTATTTCAACGTACTGCGTGA- TGCAGACCGTATCGTTGACAAGACCTACACCATCAGT CAGTCTGGTGCAACCATTCAGGGCTTCACCCTGTCCAGCTACAACTGCCCGGTAATTCCGTCTAACCGTTTCC- CTAAATATTCTCAAGGTCAAACTCATCACCTGTTGTCC AATGAGGATAACGGCTATCGTTATGACCCGCTCCCGGCAATGAATGGTGCTATCGCTGTCTTGTTTACGGCGG- ATGCGCTGCTGGTTGGTCGCTCTATCGATGTGACTGGT GACATCTTCTATGAGAAGAAAGAGAAGACCTACTACATTGATACCTTCATGGCTGAAGGTGCAATCCCTGACC- GTTGGGAGGCTGTGTCTGTTGTTACAACCAAGCGCAA CACCACTACTGGAGCAGTAGAAGGCACTGATGGTGCGCAGCATACTATCGTCAAGAACCGAGCACAGCGTAAG- GCTGTCTATGTCAAGAATGCGGCACCTGTAGCTGCT GCTGCCGCTAGCCTGTCTGCTGAAGATCTGGTTGCTGCTGTTCGTGCTGTGATGGCTAATGACATCAAGCCGA- CTGCACTGAAGCCGACCGAGGAATAACCTATGCCCTA TCTACCTTGCGTAGGTAGGGTTCTTTTGTTTAGGAGGATTCATGCCTGTAATTCAACAATCAAGTGATGTAGG- TTACATCATGTCCGATGCAAGCTTTAGCATCATTGATA GCAAGCTAGAGGCCGTCAACCTTTGTATGCGGGCCATTGGTCGTGAGGGTGTGGATTCCCTTGACTCAGGCGA- CCTTGATGCTGAAGATGCAAGTAAGATGTTGGACATT GTGTCACAGCGCTTCCAATATAATAAAGGTGGAGGTTGGTGGTTTAATCGTGAGCCTAATTGGCGCATCGTGC- CGGACACTAATGGCGAAGTTAACCTGCCTAATAATTG CCTAGCTGTCTTGCAATGTTATGCATTAGGTGAGCGTAAAGTTCCTATGACAATGCGTGCAGGCAAGCTGTAC- TCCACATGGAATCATACGTTTGATATGAGAAGTCATG TGAACAAAGATGGTGCTATTCGTCTGACACTTCTGACATATCTACCTTTCGAACACCTACCTACTAGCGTAAT- GCAAGCAATCGCATATCAGGCTGCGGTGGAGTTCATTG TATCTAAGGATGCAGATAAGACCAAGTTGACCACCCATCAGCAGATTGCAGCACAGCTATTCGTTGATGTTCA- ATCTGAACAGATGTCCCAGAAGAGACTCAACATGTTA GTACACAACCCTACACAGCGTCAGTTTGGTATCATGGCAGGTGGATCTCAGAACGTACCAGCTTACTCGCATT- CACCTTACGATGGTCATCCACTTAAACCTTGGGAGAG TTATCGCTAATGGAAGTTCAAGGTTCTTTAGGTCGCCAGATTCAAGGCATAAGCCAGCAACCTCCAGCAGTAA- GATTAGATGGACAGTGTTCAGAAATGGTTAACATGGT GCCTGATGTAGTGGAGGGAACCAAATCCCGCATGGGTACAACGCATATTGCCAAACTCTTAGAATATGGTGAA- GATGACATGGCAGTGCATCATTACCGTAGAGGGGGT GAAGGTGAGGAGGAGTATTTCTTCATAATGAAGAAGGGTCAAGTACCTGAAATCTTTGACAAACAAGGACGTA- AGTGTATGGTGCAATCACAGGATGCACCTATGACCT ATCTTAGTGAAGTGACTAACCCTAGGGAAGATGTGCAATTTATGACTATTGCAGATGTGACCTTCATGTTGAA- TCGCAAGAAGATCGTCAAGGCCCGACCTGAACGCTCC CCTCAAGTAGGTAGCACTGCTATTGTCTTTATGGCCTATGGTCAATACGGTACGCACTACAAGATTATTATTG- ATGGCGTAGTGGCTGCTGGCTATAAGACTAGGGATGG TGCCGAGGCACACCATATTGAAGACATCAGAACTGAAAGCATAGCTTACAATCTGTACCAGTCACTCCAAAGT- TGGGATAAGATTGCAGACTATGAAATCCAGTTAGAT GGCACCTCAATCTATATCACAAGGCGGGATGGCTCTACTACCTTCGATATAACCACAGAAGATGGGGCAAAAG- GTAAGGATTTGGTAGCCATCAAGTACAAGGTGGCAT CTACAGACCTCTTACCATCACGTGCACCAGAAGGCTACAAGGTGCAAGTCTGGCCTACTGGCAGTAAGCCTGA- ATCTCGGTACTGGCTGCAAGCTGAGAAGCAGAATGG GAACATTGTCTCTTGGAAGGAGACACTGGCCGCCGATGTGTTGATAGGGTTTGATAAGTCAACCATGCCTTAC- ATTATAGAACGTACAGGGTTTGTTAATGGAATTGCGC AGTTTAAAATTAGACAAGGCGACTGGGAAGATCGCAAAGTAGGCGATGACCTGACTAACCCTATGCCTTCATT- CATTGATGAGGAAGTGCCTCAGACATTAGGTGGTAT GTTTATGGTGCAGAATCGTCTATGTGTTACTGCTGGCGAGGCTGTAATTGCAACTCGCACATCTTACTTCTTT- GACTTCTTCCGATATACCGCCGTATCTGCTGTAGCCACT GACCCATTTGATGTATTCTCAGATGCGAGTGAGGTTTATCAGCTTAAACACGCGGTTACATTGGACGGGTCTA- CTGTCTTGTTTGCAGATAAATCTCAGTTCATCCTTCCT GGAGATAAGCCTCTTGAGAAGTCAAACGTATTGCTCAAGCCTGTAACCACATTTGAAGTTAACAATAATGTCA- AGCCTGTAGCTACAGGTGAGTCCGTAATGTTTGCTAC AAGTGAAGGTGCTTACTCAGGCATAAGGGAGTTCTACACAGACTCTTATAGTGATACCAAGAAGGCACAAGCA- ATAACTAGTCATGTCAATAAGTTGCTAGAAGGTAAT GTTATTATGATGTCAGCCAGTACTAATGTGAACAGGCTGCTTGTCTTGACCGACAAGTACCGAAACATTATCT- ACTGCTATGACTGGTTGTGGCAAGGAACCGAACGTGT ACAAGCTGCATGGCATAAATGGGAGTGGCCTTTGGGTACGTTTATCCGTGGCATGTTCTATTCAGGTGAGCAC- CTATATTTGCTCATAGAAAGAGGCAGTACTGGTGTGT ATCTTGAGCGCATGGACATGGGTGATGCGCTTGTATATAACCTGAATGACCGCATCCGTATGGATAGGCAAGC- TGAACTTATCTTTAGACATATCAAGGCAGAAGATGTG TGGGTGTCTGAGCCGTTACCTTGGCAACCAACCGATGTAACATTGCTTGACTGTGTACTGATAGATGGGTGGG- ACTCTTACATAGGCGGGTCTTTCTTGTTTAGCTATAAC CCAGGCGATAACACCTTAACTACAACCTTTGATATGCACGATGATGACCATGTGAAGGCTAAGGTAGTAGTCG- GCCAGTTATACCCACAAGAGTTTGAACCTACACAGGT AGTAATACGTGATAACCAAGAGAGGGTGTCTTATATAGATGTGCCAACGGTGGGGCTTGTTCACCTAAACCTA- GACAAATACCCTGACTTCAAGGTTGAGGTCAAGAATT TGAAGAGTGGCAAAGTACGTAATGTGCTGGCCTCTAACAGGGTGGGTGGTGCCATAAATAATATTGTTGGCTA- TGTAGAGCCGAGAGAAGGTGTGTTCAAATTCCCACT AAGGTCTCTTAGCACCGACACAGTTTATCGTGTGATGGTAGAATCGCCTCATACCTTCCAGCTTAGGGATATT- GAGTGGGAAGGTTCGTACAACCCTACTAAGAGGAGAG TGTAAATGGCAATAGGTACTGCTCTTACAGCAGGATTGTCCAGTGTAGCAGGTAGTGCTGCATCTGGTGGTTT- CCTGTCTTCGTTGGGTGGTGCTATAGGTGCAGAAGGG GTAATGGGTTCTGCCATGAGTTTCTTAGGCGGAACCACTGGAGGCTTCTCTAATGCTGGCCTCCTGTCGGCAG- GTATGCAAATGCTTAACCCGATAGGAGACTACTTCAC GCAGAAAGAAACAGCGAAGGCGATGAAGAAGGCGCAAGATGAGCAATGGCGTCAGCAGTTGATAGCCACAAGG- GAGGCTTATGCTTCCGTGGCTAATGCTGAAAGGTC TGCCTCTAAGCAATACCATTCTGAACTAATAGACAATCAGGTATCCTTATTACAGCAACGAGCACAAGTTGCC- TTGCTTGCAGGTGCGAGCGGCACAGGTGGTAACTCTA TCACCTCTATGCTGAATGACCTGACAGGTGAAGCTGGTAGGAACCAAGCCACCATTATTGACAACTATGAAAC- ACAGCAGATTAACTTTGCTAACCAGCTCAAGTCTATC CAGAAAGGTGGTCAGATGATGATGCGCTCCTTTGAGAAGCCATCTGCATTCAGTGCCATAGCCAAAGGTGTGT- CTGGTATAGGTGAGGCTTACCTGTCTGGTCATCAGAA GGGTACAGCACTTAGCAAGGCTTGGTCTGACTCTAGGACATATTCATCAGGAACAAGAGGAGTTTAAATGGCA- ATTGAACGTCAAGCTGTACAGGGCTTACGCCGAGTG CAGTCTACTGGTGGGCCAAGTGCTGCTAGTTTTGCGACTCGTCAGGTTGGGGTGCAAGAGACTAGTGCATCTG- GTAGCCGCTTTCTTGAAGACCTTGTAAATGCTGCTGG CAGTTTGGCGACTGTCACTACTTCTATTCTGAACCAAAGAGTGGAAGATGATAAGGTAAGACAATATAATAGG- GCGCTTACTGGCCTAATGCCAACTGAAGATGCAACG GTAGGCGGCGCACGCGCACACATGCTTGTTAGTCTACAAAATGACATCATCGCGCAAACTATGCAACTGTCCG- ATGATGCACAACGCTTTGATGGCGATGACAGTCAATG GGAAGATCACGTCATTAATGCCCGCATGGCTGTGCAAGACCGCCTATGGGATACCTACCCTGAACTTCGTGGT- GATAAGGAGTCCATGCGGGTAGTTACTAATGCCTTCA TGGAGCAGCAACCTAAAATATTTGCAGCAAGGGAGACCGCCAAGCTGAAGCAGGAGGCGGAAGCCCGCATCAA- GTCTATGGAGTCACGCATTCTGCTGGCTACCCGTGA TGTTCCTGGCGAAGCTATGGGTGATGCCTTGAATCAGTTGCAGAAAGAAGCTATGGCTATGCAAATCACCAAG- CAGGAGTTTGATGCACTGGTTTCTCAATTGGCAGCTA ATCGTGCAGCTATTGGTGATGATTCTATGATTCAAGGAACCAAGTCTCTTAAGGATGAGAATGGAGTATCACT- CTATGACCGAGTAGGTCAGTTACAGACAGGAGAGATT CAGGCCAACCGCACATGGGCGGCGCAGAACCAAGTGGCACTCTTTGAGAAGAAGGATGCTGCAATCAAAGCCT-

TTGAAGCTGGACAGCTTAACCGCGAACAGCTACTTC AGGTCATGCAGAACCACAATGAAATCTCAGGAGGCACCGCTTGGTCTGATAGCGAGATCAAATCTTTATTTGA- TAGACAGGCTAAGGCTCGTGCTACGTCTGCCAAGCTG GAAGATTTGGTGGCCCGTGGTGAACATGGCTCACCCCTAGGCTTGCAAGATATCAGTAAGGAAGACCGCAAAG- CGTATGCTGGTGCATTGGTTGATGCCTACACCAAGTT AGCCAATGACGAGATAACCCGTACAGGAGCTACTGGTGAAGAAGCTGAAGCTATCCGTGGCCGCTATGAGCAG- ATGCGATATGCCAAGCTGGGCCAGCAGTTGATTGAA GACCCTATCATTAAAGAACGGTACGGCTCGCTGATGCAACTCTCTTCTGCCAACCTCAAAGATATGAAGATTG- AACCTGAAGCATTGCAGACTATTATGCGCGCCCGCGA TTCTATCCCGGAAGATGCCCGCCGGGCGGTGATGGGTGACAAGGAGTACGCCTTTGCGGAGAATTATGATTTG- GCGACACGCATGGGTTACACTCCTGGACAGGCTATA GAGTTTGCACAGAATGCATCGCGTGGGGACAAGCTTCCCGGTTCTGTTATGAAAGAATTGAATGATGAAGTAG- ATGGTGTGGTTAGTGATGTTGCGAGCGGTAGCTGGCT TACGCGTGGCGACAACATGAGTGACATGGGTCGTGACCTTATGCTAGAAGAGGCAAACCAGATTGCTCGCTCT- ATGAAGGTTGCAGGTCATAACAATGACACCATTAAG CGTCATCTCAAATCTTTCCTACAGAATCAGTACACTCAGCTATCTGAAGGTTTCTTCACTCAAGGTGTTCTGG- TCAAAGGTGATGTGAGGACGCTAGGTGACACTATAGGT GCCAACCAAAAAGACGTACCTACGGTATTACGTCAGTACCTTGACAATCATAAGCAAGCATTGCTGGATGCAT- CTGGCGGTATGGAAGAAGGAGACTTATACTTTGATGT AGACTCTAAGCGCGGTATGTTTACAATACGTGCTGGTTCTGGGCGTGTGCCAGTTACTCCAGCTATGCCTTTG- TCTGAAATCAAGGGACAGGACTTACTGAAGGAGCACT ACGAGAAGGCAGTTAAAGAGCGCGATGAAGCGAAGAAGAACTTTGAAGCTAATCAGATGCGTATGTGGGGTGC- TGGTGGTTACCAATCTCCTGCACCAGAAAAGACTA CAGCTAAGACTGTAGGTTCCCGTGGCATCGCTGACTTCCTCATGTCGCCTGCCTTTGCATCCGGTGAGAATCT- ACCTTCCAACTTTGAATTCAACTACAAGAGGAATAATA TGGACTTCTACAATTATGTAGCTAAGACCGAGAATGGGGCCAACGTAGGGTTTGACCGAGTAGCTGGCGTGTA- CACTCCGTACAAAGATGCACACGGTCAGTCTGTAGG TTATGGTCACTTCCTCACGGAGGAGGAGAAGAAGAATGGATACATCACTATTGGCGAAGATAAAGTACCATTT- GCACCGGGACAATCTCAGTTAACACCTGAGCGGGCA ATGCGTCTGCTTGAGCAGGACATGAAGAGCCACGTACCTAGCACAAAGGATTGGGCTGTACCTTTTGATGCAA- TGCATCCGGGAGTGCAACGTGGCCTCATGGATTTATC TTACAACTTAGGAAAGGATGGCATCAAGAATGCACCGAAAGCCTATGCAGCCTTCAAGGCTGGCAAGTTCACC- GATGGGTTTATCGAGATGCTGTCTACTGCATCTACTG AAGGTAAGCGTAGCTCCGGCCTGCTAGTTCGCAGGGCGGAAGCTTATAACCTTGCACAAAGCGGAGGGTCTGT- ACCTAAGATTAGCGAAGTTGAGACAAGGGAAGATGG TTCCATGTACGTTAAGTTCTCAGGTAGCATGTCAGAAGCATTTGTGAGCAAGTCTATCCTTGGTAAGATAGGT- AAAGATGGGTGGATGGAAGTCTACCCTCCTAAAGCAG GAGCACTTGCAAGCGGCACCAAAGTGGGTCGTATTAAACTGTAGTGTCATACTCAAGGTTGTCTAACATGTTG- GACAGCCTTTATGAATGACATTAACTAAGGAGGTAAC ATGGCTGACGATATTAGCCAAAGCTGGGTGACGGTATCTCAACGCAGGTTGCCGCCTACCTTTGCACAAGTGG- CAGAAGCCGAGCGTAAGCTTGAAGAACAAAGAGCTA GCGATAAGGTTATGCAGACTGCACTGGAAAGCGAATGGGCGCTATACGGTGGTCAGCGTGCTATTGAGCGGCA- TACAACTGAGTTTGCCGAACAAGAAGGCTACACGGT TCCTGAGTCAACAAAAGATGAACTATCAAAGATTCATGGTTTTGAAATTGCACAGGATATTGTGAAGGATGTT- AAGTCACCAGAAGAGTTGCAGTTTCGTATGTCCAATG CTATGGCGGATAAGGAGCGATCGGAGATCCTTGCACGTAATGGGTTTACAGGGTTTAGCGCTCAGTTAGCTGC- TGGTATCTTCGACCCAGTTGGTTGGGCTGCCTCTATG GTTGCCGCCCCTGTAGCTGGTGCAGTAAAGGTTGCCCGTGTCGGTCGTATCATAAAGACGGCAGCAGTGGCTG- GTGCCGAGAACGCAGCATTGGAAGCCATCCTAGCCA GCGGTGATTACCAGAAGGGCGCAGATGATGTGCTGGCTGCTGCTGGCTTTGGTATGATAATGGGCGGCACCAT- TGGCGCAGCTACACGCGAACGCATCGCCAGAAAGCC AGGAGTACAAGGAGTGAATGACGGTGCTGAGACCGTAGTGGATGACTTAGATACGGTCGTAAAGGGAGCAGAT- GAGTTTGATGCATCTGCGGCTAAGGCTGTACGAGA GGCTATGGAGTATGACGCGTACATGGCTGTGCGTTCCTATGAACCACTGAGGGCTAAGGAAGTGGATATGGAT- GTAGCAATCCTGTCTCACTTAGATGACCTGAAGGCTA ACTCTAGCGTGCGTATGAGTGCCTCCGAGAAAGGTAAACTGAAGGAGCAGATACGCCAGCTTGAAACAGAAGC- CGCCACCATTAAAGGCAAGAAGGTAGATGCCGTGG CAGAAGCTGCTGCTGCTAAGGGTGCGCCTAAGTCTGCTGCTGATAGGCTAGACTTGGATGTTAAGAAGAAGGC- ACTGGCACGTCGCTTTGATGAGCCGCTTGCCGACATC CAAACAAGACTCGACGAACTTAATGCTAAACTGGCCCGCGTGGAGAACGTAGGTAAGTCAAAGGAGGAGTTGA- AGAGATTCTCTAATCTAACTAGGGAGCAGCAAATC AAGGAGCTAGGGTTAGATGCTCCGGCTCGTAAAGTTGAGATGACAAGTGCGGTACGGGAGGCTCTTGCAGCTA- TACGTGCTGAGAAGAAGAAGACACCCACTCAGACTC ATGCCGAAGCCAAAGCACAGGCAGAAGAGGAAGTGCGGCAGAAGCGAGATGACTCTATCGGCGCTAAGCGTGT- AGAGGATTCTGAAATTGCAGGTGAACAATTTGACC TGTCTGATAGCATGGAAGATCTTATGGATGACCTTGCACGTGAAGCATATCAGTCTGAAGTTAGACCTGTAAA- CCTCAAGGGACTTGGTTCTGTATCTTCCGTGATTCTGA ACTCAAAGAACCCTGTGTTTCGGGGTCTTGGTTTGCGACTGCTGGAGAATGCACAAGGTGGTGCCTACCAAGG- TAAGACCGCTTCTATCTTGTCTAACGTGTATGGTAAC TTGATTCGCTTTGCTGAGAAGAATCGATACAATGATGGCTTCTCTCAATTCATCAAGGATAACAATTTACGTG- CTGTTGATTACCTGAACCCTGCTGTTACGAGGGATTTT AATAACCAGATTTATACTGCTATTGTCAAAGGAATACCTGATGACACGCCACGTGGTGTTAAGCTTGCTGCTG- AAGGCATCGCAGATAAGCTGGCTAAGTCTCTTGAAAT CAGAAAGGCTGCTGGTGAGAAAGGCTTCGAAGATGTCAAGTCGGCACGTGATTATATCCCTGTGATATATGAT- GGTATCAAGGTGACTGAAGCAGTCAATAGACTTGGT AGTAGCGAGGCTGTTATTGCCCTGCTGTCCAAAGGTTATCAGACTGGTAAGTATAAGATGGGTAAGAAGGCAG- CGGATGCACTGGCTAAGGTGCAGTATATTCGCGCCT CCGATTCTACCTTATCAAGCCGTGTAGCCTTTGACAGGGTAGTTTCTCAGCAGCAACAAGCACAGCTTATTGA- AGACCTGAAGAGAGCAGGTGTGCCTGATAATATCATA GATAACTTCATCGAAGGCACTGAGTTGCAAGAGATGGCGGAATCAGTGTCTAACCGAGCTAAGGCAAGCATGG- GTATCAACACTCAGGCTGAATATGGCGGGATGAAG GTTCAGGACTTGCTCAACACTAACGTAGGTGAGTTGGCGGAGAACTACGGCAAGGAGGCAGCAGGTGGTGCAG- CTTTGGCGGCTATGGGGTTCCCGACCCGGCAGTCTG TACTGAATGCAATTGACGCAGCAGAACGCGCAGGGCGCAATATGGCGGGCGCTGACGCCAAGGCAATCAAACA- GCTTAGGGCGGAATCAGAAATGCTCAGGGACTCCG TGAAGCTCATATACGGCAACACCATTGACGCAAATCCAAATGCGGGTATCGTCCGAGGGACTCGCCGTGTACG- TGAGATCACTGGCCTTCTGCGTTTGGGTCAGATGGGC TTTGCGCAGGTGCCGGAGTTGGCCCGCGCCATTACCAAGATGGGAGTAGGTACAGTGCTGAAGTCTATCCCTG- CCACTAAGTTCTTACGCTCCCGCGCCGGGCGTAAGGG CGGGACAGCACAAGGTGAGCTACTTGAGCCGGAACTGCGAGAGATGGAAGAACTCATAGGTTATATCGGGGAA- GATAACTGGCTATCAGGTTGGAACGTAAGGCACGA TGAGTTCGGAGAGACCGCTGACAACATGGGACGTCTGTCTGCCATCATCGACAATGGGTTGGCTATGGGTAGC- CGTATTAACACATGGCTGTCTGGCTTCAAGGCGATAC AGGGTGGTTCTGAGAAGATCGTAGCACGCTCTATCAATAAGCGACTCAAGCAACATTTGATGGGCGAGCGAGA- GCTACCTAAGCGTGACCTTGAAGAGGTTGGCTTGGA TGAGGCTACCATGAAGCGACTCAAGCGCCACTTTGATGAGAACCCGATGTATGCCGACTATAACGGCGAGAAG- GTTCGAATGATGAACTTTGACGCCATGGAGCCAGAC TTACGAGAAATCGTAGGTGTGGCAGTGCGCCGTATGTCTGGTCGTCTTATTCAGCGTAACTTCATTGGTGATG- AAGGTATCTGGATGAACAAGTGGTGGGGCAAGGCTCT CACTCAGTTTAAATCATTCTCTATTGTGTCTATTGAGAAACAGCTTATTCACGACTTGCGTGGTGATAAGATT- CAGGCAGCACAGATTATGGCATGGTCTTCCTTGCTAGG TTTTGCATCATACGCTACACAGATGCAGATGCAGGCGATTGGACGAGAAGACCGAGACAAGTTCTTACGGGAG- AAGTTTGATACTCAGAACATAGCTATGGGTGTATTC AATAAACTACCACAAGTGGCTGGCTTTGGCTTAGCTGGGGATACCTTTGCAACATTCGGCCTTATGCCGGACT- CCATGATGCAGGCACCGGGTCGTATGGGCTTCCGTCA GCAAGGATTTGGCGACTTAGTGGCTGGTGCTGGTGTCATAAGTGATGCTGTGAACTTGTCACAGGCTTTAGTG- AAGTATGCCAATGGAGATGATGATGTCTCCACTAGGC AGTTAGTAGATAAGGTACGACGTCTTGTGCCTTTGGCAAATACGATTGGTGTAGGTCAGATGACCAAGGCCAG- CGTAGACTTATTGGAGGATTGATGAGTTATACTTTCA CAGAACACACAGCGGTAGGTTCTCAGACGACTTATCCGTTTAGCTTTGCTGGGCGCGACAAGGGTTACATTCG- CGCATCAGATATTATTGTGGAAGTGTTTCATGAAGGC GAGTGGAGTATTACACAAGGTTGGGTGCTATCTGGCACTCACCAGATTACCTTCAATGTAGCACTACCAGCAG- GGACTAAGTTCCGCATACGTAGAGATGTAGACAAAG AGTACCCTTACGCGGAGTTTGATAGAGGTGTGGCTCTTGATATGAAATCATTGAACAACTCATTCATTCATAT- CTTGCAGATTACACAGGAGATTCTTGATGGCTTCTACC CAGAAGGTTACTTCGTCAAACAGAATGTGTCTTGGGGTGGGTATAAAATTACTGACCTAGCTGATGGCACAAA- CCCTCACGATGCAGTGAATAAGGGGCAGCTTGACGC AATCGACAGGAAGCATACTGAGTGGAATGAACAGCAAGATATTGCAATTGCTGGACTCAAGGCAGGGATGACA- TCAGGTCTCTCTCATCGGACAGTACCTTGGGTTACA GTAGCCGCCGGGGGAGAGCAAGTTATTAGGCCTCCTTACATCTTTGAATCCGCCTTGGTTTTCCTTGATGGGG- TCTTGCAGCACGAACTGTCAGGTGCAGTTACTATAGCT AACAGCACCCTCACCTTCTCCGAGCCTCTACGTCGTGGCACAGAAGTGTATGTATTGATAGGTAGTCGTATTG- CAACCTCTTCACCGGGCCTGCATATGGAGTTTAATAAA GACTTAGGTGCAGGGACTACGGAGGTTAGGATTGGTATGGCTTTCTCTCATATTGATATCTACCTTGATGGCT- TGTTCCAACCTAAGTCAACATATCAAATAAACGGCGAT CTTGTTACATTCTCTGAGGGTGTACCAGCTTGCCATATGTCAGCGGATGTAGTCACTTTATAGGAGGTAAGAT- GGTTGATTCCGAACTGGTTAGCGGCGGGATGAAGTTA GCGCCATCTGCCTTAGTATCAGGTGGGTACTTCCTCGGCATCAGTTGGGACAATTGGGTACTGATTGCGACAT- TCATTTATACTGTGTTGCAGATCGGCGATTGGTTCTAC AGTAAATATTCATTATGGAAGGAGAAGAAGCGTGGCAAAACACAATAAACACGCAGCTACTGAAGACGAGGTA- GGTAAGTTACATAGTGCTATCACTAATCTTTTCAAT AAGAAAGCTGCTGCAATCCTAGCTGCGGTAGAAGAAGATCCTGATGCAGCAATTGCACTGGTTTCCGGCAAGG- ACATGGGTGCCATGTGTAAGTGGGTATTGGATAATG GTATTATGGCTACACCTGCTGCACAGCAAGAAGAGTCTGCACTATCTAAGCGCCTTGCTAAGATCAAAGCAGC- ATCTCAAGGTAAAGTAATCCAATTTGCTAAGGAGGCT TAATGGCTAGAGCAAGGGAGTCACAAGCTGAAGCCCTTGCCCGTTGGGAAGCCCTGCATGAGTTACAGCAAAC- TTTTCCGTACACTGTAGCAGGGCTACTCTCATTTGCT CAGGTTGTAATCAATAATTTAATCACTGGCAATCCAGACCTGAACCGGGTACAAGCGGATATTCTGAAATTTC- TCTTTGGAGGTAACAAGTATCGAATGGTAGAAGCACA GCGTGGTCAGGCTAAGACGACCATTGCAGCTATCTACGCTGTGTTCCGTATCATCCACGAGCCACATAAACGT- ATCATGATTGTGTCTCAGACAGCGAAGCGAGCAGAAG AAATCGCTGGGTGGGTTATCAAAATCTTCCGTGGTCTGGACTTCTTGGAGTTCATGTTGCCTGATATCTACGC- AGGTGACAAGGCTAGTATAAAAGGTTTTGAAATCCACT ACACCTTGCGTGGTAGCGACAAGTCTCCATCAGTGGCTTGCTACTCCATCGAAGCAGGTATGCAGGGTGCGCG- TGCAGATATCATCTTGGCGGATGACGTAGAGTCGTTG CAGAACTCTCGTACTGCCGCAGGTCGTGCTTTACTTGAAGACCTTACCAAGGAGTTTGAATCGATCAACCAGT- TTGGTGATATCATCTACTTGGGTACTCCTCAAAGCGTA AACTCCATCTACAACAACCTCCCTGCGCGTGGGTATCAGATTCGCATCTGGCCAGGTCGCTACCCTACACTGG- AGCAGGAGGCTTGCTATGGGGACTTCCTAGCGCCGAT GATTCGTCAGGACATGATTGATGACCCAAGTCTGCGCTCAGGCTATGGCATAGACGGTACACAAGGCGCGCCG- ACTTGTCCTGAAATGTATGATGACGAGAAGCTCATT GAGAAGGAAATCTCTCAAGGTACAGCTAAGTTCCAGTTGCAGTTCATGCTGAACACGCGCTTGATGGATGCCG- ACCGCTACCCTCTTCGTCTTAATCAGCTTATCTTAATG AGCTTTGGCACTGACGTAGTGCCGGAGATGCCGACTTGGAGTAATGATTCGGTAAACCTTATCAGTGATGCGC- CACGCTTCGGGAACAAGCCCACAGACTACCTGTATCG GCCTGTGCCGCGTCCGTATGAGTGGCGGCCTATTCAGCGTAGGTTGATGTATATCGACCCGGCAGGTGGAGGT- AAGAACGGCGACGAGACGGGTGTAGCCATTGTGTTCT TGCTTGGAACCTTTATCTACGTCTACAAAGTCTTCGGCGTACCGGGCGGATACTCAGAATCGGCCCTCAGTCG- CATTGTGAGAGAGGCAAAGCAGGCGGAGGTAAAAGA GGTCTTCATAGAGAAGAACTTTGGTCATGGTGCGTTTGAGGCGGTAATTAAGCCATACTTCGAACGCGAATGG- CCTGCCGAGTTGAAAGAGGATTACGCCACTGGTCAG AAAGAGGCCCGCATCATTGAGACACTTGAGCCTCTTATGTCCGCACACCGCATCATCTTTAACGCTGAGATGA- TCAAGCAGGACATCGATAGCGTTCAGCACTACCCTCT TGAGGTTCGCATGAGCTACAGTCTATTTGCTCAGATGTCGAACATCACCCTTGAGAAAGGATGCCTGCGGCAC- GATGACCGCTTAGACGCGCTGTATGGCGCTATACGGC AACTGACCTCTCAGATAGACTATGACGAGGCCAACCGGATAAATCGTCTCAGGGCGAAGGAGATGCGCGAATA- TCTGGAGATGATGACCGACCCTCTACGTCGCCGGGA GTTCTTCACTGGACAAGACCACGGGTATCGCAAATCAACTAACGTGTCCAATGCGATGCAGTCTAGGGTGTTT- GGTGGTAGCCGTGTTAAAGTGAAATCCAGAAATACCA TTTCTTCAAGAATTTCAAGGACTTGGTAATTAGGGGACACTATAGAAGGAGGCCGAGGAATAACAGGAAGTTA- TAGGAGGTCATAGGTATTCCTAGGTAGTATAGGTAC GCCTTAGTGGGAGGTATCCTACCTCCCTATTCCTTCCTTTATATTAACTATAGATAAGGAGTAATAATGCCTA- ATCGTCCTAATAATTATGGTAATATGGGTCTGACAGGT AAACCTCGTCGTAAACAAGAGAAGCCTATTGCCACTGCACTGATGGTTCCTTTTGCAGAAGATGAAGCCCATG- AGCATGGTGAGAACATCGAAGTACGTGAGAACCGCA TTAATGACCAGACCAAATCAGGTAAGCGCCGTGGTGCTATGCTGCTGACAGACAAGCATGGCCTTGTGGTTGC- ATCTGGCAGCCGCTTCAATGACATCTGGTATAGCTTT AAATTCGAAGAAATTGGTACAATTCAACCTGCATAAGAAGGAGATAACATATGGCAACTATCAAATACGGTGA- TGCTGGTACTGCAACTGGTAAGGCTTTCCTGAAACA GCAACTGGAAACCACAGCGACTGCACTGCCACTTCCAATCGTGTCCAAGTCAGACTTGGGTCGTGCACTGGCA- CCTATCAATCAGGCTCGCCTGTCTGGTAAGCAGAAGG GTGCTATGGTAATCATGGAAGATGACGGTACGCATGAACTGCACATTGCGGTGGCTGATGGCCCGCTTCCGAC- TGACGCATGGAACATTTGCAGCCTTGACGGTGAAGTA ACTCCGGCACAGGGCCGCTAAGGAGGCTAGATGCTACGACATCAGATTAACGGGAATCACAACCCGTTACATG- TAACAGGCCAACGCTCACGGAGTAATAAGAGTATTG CCATCCAGGAGGGTGTGCCTATTGTACGTGCTTCTGTTCTAGCATCTCCGACATCTTACATCAATGACCCTCA- CCTGTCAGGTAAGCGTGAAGGTATGATGGTGGCTGTAC TGGCACCTGAAGATGGAGACAAGGCAGGTCTATATCTCTACAGGTGGGCCAGATAAACATAACATCATTGACC- ATGCGGTGTTCAAACATTTTGTAACTAACGGCTTGGT TGTAGGCGCTATTGAGACGCACACTGCCACCACTAACAACATCCATGTACGTATGCACATCACAGAAGGTTCT- ACGGGTGCATACACCTTTAGCTTTTCCTTTGAGTGGA CATCTGACTTCGACTTACTGGAGTGATAATGTTGAACAAATACTTCAAGCGTAACGAGTTCGCTTGCCGTTGT- GGGTGCGGTACATCCACTGTTGACGCGGAACTGTTGC AGGTTGTCACAGATGTCCGTGAATACTTCGGGTTACCTGTAGTTATTACATCGGGTCATCGGTGCAGTGACCA- TAACCGCCGCGTAGGTGGTGCTGCATCTTCCATGCACA TGACTGGCAAGGCTGCTGATATTAAAGTGAAAGGGAAGGACGCGAGTGCTATCGCATCCTACTTGGAACACAA- GTACCCTGATAAATATGGTATCGGTCGATACAACTC CTTCACTCACATTGACGTGCGTGATGGTAAGGCTCGCTGGCGTGGATAACTGCATTGCATGGTGTGAGAAGAT- GGTTGCTAAGGCATCTGCTGAAGGTAACTATGTTGAC TGGCAGAATTACACCAATCTGCTTAACGAATGGAAATGGAGAGCATTACGATGAAGAAACTATTCAAGAGCAA- GAAGGTGATCGGCGCACTAGTTACACTGATCGTTGC GCTTGTATCGGTATGGCTTGGTGTTGACCTAGGCTCAGGTGCGGAGTCTTCTGTTACCGATGTGGTCTGCCAA- GTAATTACCTGTGAGTAGGTTACTTGAAGTAGTGGCAG GACTTCTTGGCCTGCTGCTTGCCTATAAGAAGAAGCAAGACCAGAAGGAGGCGCAACATGAAGCAGATCTGGC- TAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTC CGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGACGGCAGTTTACGAG- AGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCT ACACAATTAGGCTTGTACATATTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACA- TACGATTTAGGTGACACTATAGAATAGAAGTATAGTG CCGTTCTTTTGAGCGGCCTATTACTCACCAGTCTTCACGGGGAGGGCTGGATAGTAATAGGAGGTTTAATGTC- ATTAACTAAACCACGTTGCTTCAGGAAGGCAAGTTAT CTAAGCCAGTTAGGCACTTTGCAGAATCTGGCTAACACTGGAGATGACGTACTTGTTATCGATGTTGACTACA- AGTTCACCAATGGAGAGACTGTAGACTTCAAAGGTCG ATTGGTTCGTATAGAATGCGAAGCTAGATTCATAGGCGATGGAGCTTTAATTTTCACTAATATGGCTAGTGGT- TCTGTAGTAGAAAAGCCTTTCATGGAGAGCAAGTCCA CACCTTGGGTTATCTACCCTTGGACAGAAGATGGCAAGTGGATTACAGATGCACAAGCTGTTGCTGCTACGCT- TAAACAATCTAAGACCGAAGGATATCAACCTGGAGTC AATGATTGGGTCAAGTTCCCAGGACTTGAAGCATTGATACCGCAAGAGGTGAAAGACCAGTATGTAGTATCAA- CACTGGACATCCGTGATTGTGTAGGTGTTGAGGTTA GACGTGCTGGTGGGCTTATGGCAGCTTACTTGTTCCGCAACTGTCATCATTGTAAGGTAATTGATTCTGACAC- CATCATTGGTGGTAAAGACGGCATCATAACCTTTGAAA ACTTAGGTGGTGAATGGGGTATCGGCAACTATGCCATAGGTGGTCGTGTACATTATGGCTCATGTAGTGGTGT- GCAGTTTCTTCGGAACAATGGAGGTGCATCACATAAT GGTGGAGTTATTGGTGTGACCTCATGGCGCGCAGGTGAGTCTGGGTTTAAAACATGGCAAGGTTCTGTAGGTG- CAGGTACATCTCGTAACTATAACCTTCAGTTCCGTGA CTCAGTTGCATTATCTCCAGTATGGGACGGCTTTGACTTAGGCTCAGACCCTGGAATGGCACCAGAAGAGGAT- AGACCGGGAGATTTACCTGTATCTCAATACCCCATGC ACCAGTTACCTAATAACCACATGGTTGATAACATACTTGTTATGAACTCATTAGGTGTAGGTTTAGGTATGGA- CGGTAGAGGTGGTTATGTGTCGAATGTTACCGTGCAG GATTGTGCAGGCGCAGGTATACTTGCTCATGCATTCAACCGTACCTTCTCTAACATTACGGTGATTGACTGCA- ACTACATGAACTTCGATTCAGACCAGATAATCATCATT GGTGACTGCATCGTGAATGGCATCCGAGCAGCGGGTATTAAGCCTCAGCCATCCAAAGGCATGATCATCAGTG- CACCTCACTCAACCTTGAGCGGTATTGTGGGTAATGT GCCGCCAGACCGTATTCTTGCAGGTAACATCCTTGACCCTGTGTTGGGTCATACAAGGATTAATGGGTTTAAT- AGTGACTCGGCGGAACTGAGCTTCAGAATCCACAAGC TTACCAAGACCTTGGATAGTGGTGCTATTCGCTCTACGCTGAACGGTGGGCCGGGTACTGGTTCTGCATGGAC- TGAGATGACTGCAATTTCAGGGTCAGCTCCAAATGCT GTCTCGTTGAAGATTAACCGAGGAGACTTCAAGGCAACTGAGATACCAGTAGCACCTACTGTGCTTCCAGATG- AAGCGGTAAGAGACCACAGCTCTATCGCACTTTATTT TGATCAGGAAGCTCTTTGGGCTTTAGTTAAGAAGCCGAACGGAAGCCTCACACGAATGAAGCTTGCTTAATGT- AGGCAGCGCGTTAGCGCTGCTTTCACGCGAACTTTTC TTAAAGGTTATCATAGTGGTAGCCTTTCAGAAAAGGAGGTGACATGATACAAAGATTAGGTTCTTCCTTAGTG- AAGATGCCAAATGGTATTACATTGACACAGTGGTTGC AACCTGCAAACATCATCAAGGTAGATGATGCACCATACAATGGAGACCTTATTGCTGCATATAATGCTATTCC- CGTTATAGGTAATTATGCTTTGGTTCTTACCAACCACA CTTACAATGCAGTTGGTTTGTTTGATGCAGGTCGTAACATGAAGCCTAACATCACCATCATTGGTGCTGGTAT- GCCTCAACTTGCAGATGATAGGTCGTCCTTTGTTGAAG GTTCTGGCACTATCATTAAAGGCGCAGTCAAGAACTCCGCCAAGGGCTTCCAGATTGGTAACCTAGGTATTGA- TTGTGGTAACACAGTTAGTCGTACAGACTACCAACCT GCACGCTTCGAAGACCCACTACAGATATACGGGTGTGGCGCTAATGCTAACATCTTTATCGATAACGTGAAGT- GCCTTAGTGCAGTTTCTGTAGACGAGAGACCGGGAAC

ACACAGCATTCTGCTTGAGCAAACTGAAGGTGTTACTCTCGGATATGTAGAGTGCATTGGTGGCTTCCACGGA- CTTACCATCAAGTGCCGTAACCTACGTGGCGGGATTG CACATTGCTATGGCCAGTATGGTGATGGCTTCATCATCAAATCTGACGCTGGTGGTGCAGCGAGTCATATCTA- CATGGAGCGGATTCAAGTGGGGCATCCAGATCAATCT ATGTGGCCTGATGTACACTTAGGTGGTATCTACGATGCTCATGATGGAGTGACAATTGACAGTGTTAGTATTG- GCGAGTTGCATGTTGTACGAGGGTCTTGGGGCCTGAT ACCTGCGGATAACGCCACGGGTAGTATCACCAACTTCCATATTGGACATTATGAGTGTCACCTTACTTATGGC- AACTACTACTCCCTTGTTATCAACGACAAGGTTGTAGG TTGGACTATGGGTACTCACAACATCACGACCTGCTCAGGTGGCATCAAGGTAGACCCTGCATCGGTGTATGTA- AACATCGGAACTGGACGCTCCACCAACAACACTGAG AGTGGGTACTCTCTTGGTGGACACACCCTGATTCATGGTGAACTGATTGCAGATGCTAATGGTAAGTACGGTG- TAGAGTATACAGGTGGCCTAGGTCTTGATGTAAGTAA GATTCATGGGTTCCAGAACCATCTTGGTACTTACTCAGGCTACTCTTCTGCTATCCAATCTCTACTGTGGCCT- GACGCTGGGTTTGAAGCGATGGTTACAGGGCGCACTGT GACATTGCGTGGGTCTCTCACGAAAGGTACGACTGCATGGTGTGGTCAGGTACTCGATGCTGTTAAGCCTACA- CGAGACATTCGTATATACGCATGGGCTGTTGGTCTTG GTGGTTCTATGGTTCCAGTGGAAGCATGGATTCGTTCTGCTAATGGAGCTATAGACGTAGTAGGAAAGGACTC- GGTGGGCGAAGGGCAGATTGTTAGCTTCACTGGCAGC TACATATTCAAGTGAGGTCTGTATGCCATTAGTGAAGTCTATCAAGGAGAAGGCTGTACGCCAGAACACAGAA- GAACTCATCAAGTCAGGTCGTGACCCTAAGCAGGCT TATGCAATTGCTAAGGATGTACAACGTCGTGCCATGAAGAAACCTTCTGCATCTTAGTGTAACCAAAGGGTTG- GCTTAGGTTGACCCTTAGTGTAATCAAAGGAGATAAC ATGTATATTCCAATGGAAGCAGTAGTAGGTATCGCTTGTTTGCTAGTAGGGTTTGTCATAGGTTTGATAGCAC- AATAATGGTGGTCACAAAGTAGCCAAAGTCAAAATTT TGATATAGGCGTGTGTCAGCTCTCTCGGCCTCGGCCTCGCCGGGATGTCCCCATAGGGTGCCTGTGGGCGCTA- GGGCGGCCTGTGGAGGCCTGAGAGAAGCTCTTAGTGT GGGCCAAAGGGTAACCTGAGGCCTGCCGGAGCGAGCGATAGGGACGCGTGTAGGCCGCTTGACAGCGTGTGTG- GGCGTGGGCTA SEQ ID NO: 3-Enterobacteria phage K1-5 TCGCCCTCGCCCTCGCCGGGTTGTCCCCATAGGGTGGCCTGAGGGAATCCGTCTTCGACGGGCAGGGCTGATG- TACTCCTTGTCTAGTACAAGGGAGGCGGAGGGAACGC CTAGGGAGGCCTAGGAATGGCTTAGTGGTGGACAAGGTGATTACCTTAGTGAAGCCTCTTAGTGCATTCCTGA- GGCCATTCAGGGCGTTTATGAGGGATTGACAGGGTGT GAGGGCGTGGGCTATCTGTTCCTTTGCTCCTCACTTCGTTCGTCGCTGCGGTAGCCTGATGTGTACCTTAGGT- TATTCCTTGATGGATAGCTTAGGTTAGCCTTAGTGGATT ACCTTAGTTAAAGCCTTAGTGCTTCACTTAGTATCAGCTTAGTAGTGTACCTTAGTAAGTCTTAGTGTCTTCT- CTTAGTGATTGCACATGCAAGCATGTAAGATGCTAATA GGTCGCGGTCGGCAGACCGCTAAAGAAAGAGAATGGTAATAAGATGCAGTAGGAGGAACACCAGAAGCCTAGC- CAACCTAAGCTATCCTAGCTCTATATCTATTGCTTT TCCTTAGTCTAACACGTTAGACAACCTATCTTATTCTTAGTGATGGTAACTTAGTGTTGACAAGATAATCTTA- GTGTAATACTATGCATCACGTAGGCGGTGCTGAGGCAC CTAGTAGCCAGCTAGTAAGGCATACGAAGAGACTAGCGCTTACATTGCTCTTTAACAATTTGCTTAGTGTAAC- CTATGTATGCCGTGGTTAACTACTTATTGAATGAGGTA TTAACTATGACATTAAATAACCGTGAACTGTCCGTTCTCTTCACTCTGTTGTGCTACATGATTCGTAACAACG- AATTACTTACAGATGATGAGTTAGCCTTGTATCACCGC TTTCTTAACGAAGGTTGGACCGATACAGTTAATCAATACCGTAACATGATAGATGAGTTGAGGGAGGGTAAAT- AATGTATCAACATGAGGTATTCTTTGAATCAGCTAGC GAAGCTATTCGCTTCCGTGATGATATGATGCAAGCTGGTGTAGGCGTTGATGTGTATCACTATTTGATAGATT- ACGACACTGAATATCACCGAGTTACCTTAGTATCTGAG TATGACAACCAAGTCATTACTGAGTATCTAGGCAGTGAAGATTACGATTACGATGAAGTAATCACGACAAATC- TCTAAATTAACTGTTGACAGCCACGGCATACAAGGTT ACATTAAGCATCAAGACGGCGACGTCTTTAAACATCCCGCTCTTTAACAATACGGTTTGTGTCTTGATAGGCT- AACTAACTAACTAAGGTAATTATCATGAAAGGGTTAA TTTGTGTAGAACGTATGGTCAATGGTAAACTTGAAATATTACCACTGGAAAACCAATCTAGCTTCAAAGAGTG- GTATGGCTGTTTCTCACTGATTTAAGGTAAAGGCTGG CACTAGTCAGCCTATCAAGGCGCAAACCAAGCTCTTTAACAATTTGGATGGTAGCTTCTTAGTCTGGATAGGT- TAAACCTAGGAGATTCTCTTGAGTCTCCTATAATGTAA CCTAACTAACTAAATGAGGATTAAATCATGGAACGCAATGCTAACGCTTACTACAACCTTCTGGCTGCAACTG- TTGAAGCATTCAACGAGCGTATTCAGTTTGATGAGAT TCGCGAAGGTGATGATTACTCTGATGCACTACATGAGGTTGTAGACAGCAATGTTCCAGTTTATTACAGCGAA- ATCTTTACAGTGATGGCTGCTGATGGTATTGATGTTGA TTTTGAGGATGCTGGTTTGATTCCTGACACGAAGGATGTAACCAAGATTCTACAAGCTCGCATCTATGAAGCT- CTTTATAATGATGTACCAAATGACAGCGATGTAGTTTG GTGTGAAGGCGAAGAAGAGGAAGAATAAGGATGGAAAAGCAATATAACTTTATCTTTTCAGACGGTGTAACCC- TGAAGTGTTCCCTACGATTCGCACAAATTCGTGAGG AAGTACTAGGCACTACATACAAACTATTTAGCTGACACTATAAGAGAAGGCTTAACAAGGCGTTACTAAGGTA- GCGCCTGATTAAACTTTCACTTACTAGGAGTTGAGAT TATGAAAACCTTGATTGGATGCTTCTTGTTGGCTTCTCTTGCTCTGGCATTTACCGCTAAAGCTGGTTATGAC- GCTTATAAAGTAGAACAAGCCCAGCAAGACTGGGCCAA AAAAAAGTTCAACTTGTGCAGCAAGAGCAACACCTACGAGTACTGCAACAAAACACTAAGACACTTATGGAAA- GAGTAACTAGCCTATAGCCCACCTGAGTGGGCTATG TGATATTTACTTAACACTATATAAGGTGATTACTATGACTACTGAAAACACCCTCGTGTCTGTCCGTGAAGCT- GCAACCGCTGAAATCAAGCAACATTTAGACAATATCG GCACTTCTTACATCAAAGTAGGGGCTTGTCTGAATGAGTTACGCGGAGACTTTGAAGGTCAAAAAGAGTTTTT- AGCCTATGTTGAAGCAGAGTTTGCCATTAAGAAGGCA CAATGTTACAAGCTGATGAGTGTAGCCCGTGTCTTTGAAGGCGATGATCGCTTTAAAGGCGTGGCGATGCGTG- TAATGCTGGCGCTTGTTCCTTTCGCTGATGAAAATAT AATCATGGAGAAGGCCGCAGAACTCGCCGCAAATGGCAAGCTGGACACTAATGCCGTAAACGCCCTGATTGAA- CCTAAGAAAGAGTCAAAGGCCGAAACGGTACAATC TAAGGCTGAGACAGTAAAACCGCAGGAGAACGCGACTGAGTCCGCAGAATCACATGAAATGCAAGCGCCGCAG- GTAGTGCCACCCGCGAGCGAGCAGGAGTCCGACGA ATCAGCACCTTGGGAAGAGGAAAGCAAACCGGAAGCGCCAAAGGCAGCTCCGATGGATAACACGGCTAATACT- GAGAATGCCGCTATTGCTGGTCTGCTGGCACAAATT AAAGCACTGACTGAGCAATTACAGGCAGCCAATGACCGCATCGCCTCCTTAAGTAGCGCACGCGAAAGCAAGA- AGGCATCCGCACCTATGCTGCCGCAGTTCAAATCTT CCTGCTTCTACGCTCGCTTAGGCTTGAGCGCGGAGGAGGCAACGAAGAAAACAGCAGTTAACAAGGCACGCCG- CGAACTGGTTAAGCTGGGATACGGTGAAGGCCATG AGGCATGGCCCTTAATCTCTGAGGCAGTAGAAGAGTTGACTAAGTAACCTTATCGGTGGCATCTTCTTAGGTG- TCACCTATTAAGGTTTCTTTCACTAGGAGTAAACAAG ATGCAAGGCCTACACGCTATTCAACTTCAACTTGAAGAAGAAATGTTTAACGGCGGTATCCGTCGCTTTGAAG- CGGACCAACAACGCCAGATTGCATCCGGTAATGAATC AGACACGGCATGGAATCGCCGCTTATTGTCCGAGTTAATCGCGCCAATGGCTGAAGGTATTCAGGCATACAAG- GAAGAGTATGAAGGTAAAAGAGGCCGTGCACCGCGT GCATTAGCTTTCATTAACTGCGTAGAAAACGAAGTGGCAGCATATATCACGATGAAAATCGTTATGGATATGC- TGAACACGGATGTAACCTTGCAGGCTATAGCCATGAA TGTAGCTGACCGCATTGAGGACCAAGTACGTTTTAGCAAGCTGGAAGGTCACGCCGCCAAATACTTTGAAAAA- GTTAAGAAGTCACTTAAGGCAAGTAAGACTAAATCA TATCGCCATGCGCACAACGTAGCGGTAGTGGCTGAGAAGTCAGTAGCTGACCGTGACGCTGATTTCTCCCGCT- GGGAGGCATGGCCTAAAGACACCTTGCTGCAAATTGG GATGACCTTGCTTGAAATCTTAGAGAATAGCGTATTCTTCAACGGGCAACCTGTCTTCCTCCGCACCTTGCGC- ACTAATGGCGGCAAACATGGTGTTTACTACCTACAGAC TAGTGAACACGTAGGTGAGTGGATAACTGCATTCAAAGAGCACGTAGCGCAACTGAGTCCTGCCTATGCTCCT- TGCGTCATCCCTCCGCGTCCGTGGGTATCACCTTTTA ACGGCGGTTTCCACACTGAGAAAGTAGCAAGCCGTATTCGTCTGGTAAAAGGAAACCGCGAACACGTCCGCAA- GCTGACCAAAAAGCAAATGCCAGAGGTTTACAAGG CTGTTAACGCGTTGCAGGCGACTAAATGGCAGGTTAACAAGGAAGTTTTACAGGTTGTGGAAGACGTCATCCG- TCTAGACCTAGGTTATGGTGTACCTTCCTTTAAACCA CTCATTGACCGCGAGAACAAGCCAGCTAATCCAGTGCCGCTAGAATTTCAGCACCTACGGGGCCGTGAACTGA- AAGAAATGCTTACGCCGGAACAATGGCAAGCCTTTA TCAACTGGAAAGGTGAATGTACTAAGCTGTACACCGCTGAAACTAAGCGCGGAAGCAAATCGGCGGCAACCGT- TCGCATGGTTGGTCAGGCCCGTAAATATAGCCAGTT CGACGCAATCTACTTCGTGTATGCACTGGACAGCCGCAGCCGCGTCTACGCGCAATCTAGCACACTCTCACCG- CAATCAAATGACTTGGGCAAGGCCTTGCTCCGTTTTA CCGAAGGGCAGCGTCTTGATAGCGCTGAGGCGCTTAAGTGGTTTTTGGTGAACGGGGCTAATAACTGGGGTTG- GGATAAGAAAACTTTTGACGTGCGCACCGCTAACGT GCTGGATAGTGAATTTCAAGACATGTGCCGCGACATTGCAGCGGATCCGCTGACCTTCACTCAATGGGTAAAT- GCCGACTCCCCTTACGGCTTCCTTGCATGGTGCTTTGA ATATGCGCGTTATCTGGATGCACTGGATGAAGGCACGCAAGACCAATTCATGACGCACCTCCCAGTCCATCAA- GATGGTAGTTGTTCTGGTATCCAGCACTACAGTGCTA TGCTACGCGATGCAGTAGGTGCGAAAGCAGTAAACCTTAAGCCCTCTGACTCTCCTCAAGATATTTATGGTGC- CGTTGCGCAGGTAGTAATTCAGAAGAATTATGCATAC ATGAATGCAGAGGATGCGGAAACCTTCACTTCTGGCAGCGTGACTTTAACAGGTGCGGAGCTGCGTAGTATGG- CTAGTGCGTGGGATATGATAGGAATCACTCGCGGCC TGACCAAAAAGCCCGTAATGACACTACCTTATGGCAGCACACGTCTAACCTGCCGTGAGTCAGTGATTGATTA- TATCGTTGATTTAGAAGAAAAAGAGGCCCAACGGGC TATTGCGGAAGGGCGTACCGCCAATCCTGTACACCCTTTTGATAATGACCGTAAAGACAGCCTGACACCTAGC- GCAGCTTATAACTATATGACAGCTTTAATCTGGCCTT CTATTTCGGAAGTGGTTAAAGCCCCTATAGTGGCAATGAAAATGATTCGTCAGCTTGCCCGTTTCGCAGCTAA- AAGGAATGAAGGCTTAGAGTATACCCTGCCTACTGGC TTCATCTTGCAACAAAAGATTATGGCTACTGATATGCTCCGCGTATCTACTTGCTTGATGGGAGAAATCAAGA- TGAGTCTACAGATTGAAACAGACGTAGTGGATGAAAC GGCAATGATGGGCGCTGCTGCTCCTAACTTTGTGCATGGTCATGATGCCAGCCACCTTATCTTAACAGTCTGC- GACCTTGTTGATAAAGGGATTACATCTATCGCAGTTAT TCATGACTCTTTTGGCACTCATGCAGGCCGTACAGCCGACCTTCGTGATAGCTTAAGGGCAGAAATGGTGAAG- ATGTATCAAGGCCGTAATGCACTGCAAAGCCTGCTAG ATGAGCACGAAGAACGCTGGTTAGTTGATACCGGAATACAAGTACCAGAGCAAGGGGAGTTTGACCTTAACGA- AATCTTAGTTTCAGACTATTGCTTCGCATAATATTAA TAGGCCATTCCTTCGGGAGTGGCCTTTCTTTTACCTACTACCTGTAACATTTCATTAACATAAAAGTGTCTCA- CATGTGAGACTTATTTACCGGACACTATAGGATAGCCG TCGGAGACGGGAAAGAAAGGGAAGATAAAGGATATAAAGGAAGTAATAGGTATTAAAGGTTATATAGGTTATC- TAGGAATACCTATTACCTTCTTCCTTCCTCTTATTAC CACTCAGAGGAAGGGCAGACCTAGGTTGTCTCACATGTGAGACTTCGTATTTACCGGACAGTATAGATAAGAT- TAACTCACTTTGGAGATTTAACCATGCGCAACTTTGA GAAGATGGCCCGTAAAGCTAACCGTTTTGACATGGAAGAGGGGCAGAAGAAAGGCAAGAAGCTGAATAAGCCT- GTCCGTGACCGTGCATCTAAACGCGCTGCGTGGGA GTTCTAAGTTATGGCTATTATTCAGAATGTACCGTGTCCTGCCTGTCAAAAGAATGGACATGATATTACTGGC- AACCATCTCATGATATTTGATGATGGTGCCGGCTACTG TAATCGTGGACACTTTCATGATAATGGTAGACCTTACTATCACAAGCCGGAAGGTGGCATCGAGATAACCGAG- TTATCTATTACTGGCAATATCAAATATACACCTTCTC AATTCAAAGAAATGGAGAAGGAAGGGAAGATAAGCGACCCTAAATTACGTGCCATCGCACTTGGTGGTATGCG- TATGAAAGACCGTTGGGAGGTCATGAATGAACAAG AAAGGGCAGAGCAAGAAGCAGAGTGGAAACTTGATGTTGAATGGTTCCTCACGCTTAAGCGTAAGAACCTTGT- TTCCAGGCACATTCGCGGCGACATTTGCGCATTGTAT GATGTACGTGTTGGGCACGATGAAGAGGGTAGAGTCTCACGGCATTACTATCCGCGCTTCGAAAAAGGTGAGC- TAGTAGGCGCTAAGTGTCGCACATTACCTAAAGATT TTAAGTTTGGTCATTTAGGTAAACTCTTTGGTATGCAAGATCTTTTCGGTATGAATACTTTGTCTCACGTGTT- AGACAAGGGAAGACGAAAGGATTGCTTGCTCATTGTCG GCGGCGAACTGGATGCACTAGCAGCGCAGCAGATGCTCCTTGATTCTGCCAAGGGTACTAAGTGGGAAGGCCA- GCCATACCATGTATGGTCTGTCAACAAAGGCGAGTC TTGCCTTGAAGAGATAGTGCAGAACCGTGAGCATATCGCCCAATTCAAGAAGATTATATGGGGTTTTGATGGA- GATGAGGTAGGGCAGAAGCAGAATCAGCAAGCGGCT CGCCTGTTTCCTGGTAAATCCTATATCCTTGAATACCCCTCTGGTTGCAAAGATGCTAACAAGGCATTGATGG- CTGGCAAGGCTAAAGAATTTGTAGATGCATGGTTTAAT GCCAAGTCATCTGATGAAGTCTTTGGTAGCCAGATTAAATCTATCGCATCTCAAAGGGATAAGCTCAAGGCTG- CACGTCCAGAGCAAGGACTGTCATGGCCTTGGCCTAA GCTGAACAAGGTAACGCTAGGTATTCGTAAGAACCAGCTTATCATTGTAGGTGCAGGGTCTGGTGTAGGTAAG- ACTGAGTTCCTTCGTGAAGTAGTTAAGCACCTCATTG AAGAACACGGTGAATCTGTAGGCATCATTTCTACAGAAGACCCGATGGTCAAGGTGTCCCGTGCTTTTATCGG- CAAGTGGATTGATAAGCGTATTGAGTTACCTCCAACC AACGACCCGAAAGAAGACGGATACCGTGAGGTGTTCGACTATACCGAGGAAGAAGCTAACGCCGCCATTGATT- ATGTAGCTGATACAGGTAAGCTGTTTGTAGCTGACC TAGAGGGTGACTATTCGATGGAAAAGGTAGAGCAAACTTGCCTAGAGTTTGAGGCTATGGGTATTTCTAATAT- CATCATTGATAACTTAACGGGGATTAAATTAGATGAG CGTGCTTTTGGTGGGAAGGTTGGTGCACTTGATGAATGCGTCAAGCGGATTGGTACTATCAAAGACCGACACC- CGGTTACTATATTCCTTGTATCACACCTTACACGTCCT CCGGCAAACCGTACCCAACACGAAGAAGGTGGCGAAGTTATCCTTTCTGACTTCCGAGGCTCAGGCGCTATCG- GATTCTGGGCATCTTACGCCTTGGGGATTGAGCGTAA TACAAGAGCTGAAACGCTTGACGAAAGGACTACCACGTACATCTCATGTGTCAAAGACCGCGACCAAGGTATC- TACACTGGAACCAAGGTCATGCTTAAGGGTGACATT CAAACCGGACGTTTAATGGAACCACAAGCCCGTACTAAGTCATTTGATACAGGTGAAGCAAGGCAACAAGAAG- TACCAGATTTACCGGATACTATAGAAGAGACTACCT TCGATGAAGAAAGTGAGTTCTGATTAGTGTATTTATCAGGCTTGTCTCACATGTGAGACAGGCTCTTATTAAG- TACATTAAATAACTGGAGATTGATTATGTATAACTTAG TGTTGAATGTAGGTGACTTTGTACGCAACATCAAGAAAGATTCAAGTCGCTATCTTTGCCGTGGTGTTGTAAC- CTTTGTAGGTGAGAACCTGTATTATGTAGAATATCGCA GTGGTGTTAAGCAATATTACCACAAGAAGACAGCACATAAATATCTTGAAAAGATTGTAGAGATAAACAATCA- ATGTAAGTGCATACATGATGAGGTTTGCGATAAATG TGCTCGCCAGATGCTTAAGAATTTCCTAGCTCCTCTTTATTATGGTGCTGGTCCTCAAACACTAGCAGAGTGC- ATGGCAGAAAAGAAAACCACACTCAAGAAAGAGCGTC GCAATGTAATCACTGGTAAGACTCAAAGTGAGATGATTAAGCAATGTGGCACTGCATTAGGTGTTACACAGTT- TAATACTCGTGCATTGGGTAAATCCACAGGACAAGCT ATGGTAAAGATTGGAGAAGCCATGATGCATCCAAATGTACCTGTGCGAATCATGGATGTTGACCATGCAATCA- CAGAACAAGGTACGCAACGACGTGTAATTAATAAGC ATTTTGCCGACACTATAGAAGGCATTATTCGTAAGCAAGGGTTGAAAGGTCTTCACATCTTAAATGGTGAAGA- ATTACTGTACCTACCTATCGTTACTGAAGAAACATAC GTGAATATCTAAGGAGTTAATCATGACTAAGGTATTAATTTATATGCGTGGACCTCATAAATGCTATGCAGTT- GTAGCACCAAATGGTGTTAAGCCTTATCGTACTTCAAA AAGATTGGCATTAATAGGTGCTAGTAGTAGTGCAAGTTTCCAAATGGAACTTTTTGGTCATTGGACTGAAAGG- CAATTCCGTGAGGATTTTAAAGTCATTGGCAGCTTCA TGGTGAAATATGCAGAATAAACATAGTCTTAGAATGTTCGATGGTCATGAAAACCTGCAAGCCAAGATTACTA- ACCAAGCCTTCCTGTTCGCACAGTTAACTATGGCTGA GGCTAAGAAGAATAGTCTCACTCGTGAACAGGTTATCAAGGAGGCCACTTGGGAACCACACCAAGGTAAATAT- ATGGGCCACAAATTAACTGTAACACGCAGTCGATAA GTCAAGGGTTGTCCAACGTGTTGGACAGCCTTTCATCATATTGATTGGGAGGTATTAAATGACTAAGTTTACT- ATGCAAGACCTCATTAAATTACGTGATGAAATAGAAT CACCGGAAGTTAATACAGAGTTTCACTACATTGATCCACGAGATAAACGAGAGATTCCTGATTATCAGATTGA- GACGGAGTTAATGTATGAAGATTATTGATTGGAAGA AGGAAGCAGAAGGCCGTATCCTAGTGATGGATGCGGAGGCTAAAGGCCTGCTGGGTGCTATCCGCTACGGTCA- TCGTGAAGATGTACACATTATTTGCTGCATGGACTTG CTCACCACTGAGGAGTTCCTCTTCTTCGACCCATATGAGATGCGTGACCCTGAAGCAAGGGAACACTTGAAAG- AGTGGGAAGGCCATCAAGATGGGACCTTGGTTGATG GTGTTAACTTCCTAAAGCACTGTGAAGCCATCGTCTCACAGAACTTCCTAGGCTATGACGGGCTTCTCTTTGA- GAAAGCCTTCCCTGACATCTGGAAGGGATTTAACTACA CCGAGAGGCGCGGCAAGGGCAGACTACGTGCTGACTTGTGTCCGGTACGCGTCATGGATACGCTGGTCATGAG- TCGCCTGTTAAACCCAGATAGACGCCTTCCTCCGCAA GCATATGCCAAAGGTATGGGTAACGTTGCCCCTCACTCAATTGAGGCGCACGGCATTCGTATAGGCCGTTATA- AGCCGGAGAACGAGGATTGGTCTAAACTAACTGACC ACATGGTACATCGTGTACGCGAGGACGTGGCGATAGGCCGTGACCTATTCCTCTGGCTATTTAACGGAGAATG- GACGGAGCACAAACGCCGTGGCGTGAATAAACGCAC TGGCCTAGGTATTGAGACAGCCTTCCACATGGAGTCCATTGTGACGCTGGAGATGAGCCGTCAGGCCGAGCGT- GGATTCCGTCTGGATATAGATAAAGCATTAGCACGAT GCGAGGAATTGGACGCTAAGATTGATGAGACAGTCGCAGCGTTCCGTCCGCACATGCCTATGCGTATCAAGTC- TAAACCTTTTAAACCGGAAGAAAAGAATGAAGTATG CCAACGCGCAAATGAGTATGGAGCTAGCAACAATATACCTACTGTCCTTGACCCCTCTCACTTTCTTCACGCA- GAGAGACGAGGAGATCGCAAGACAGTATGGAGTGTC ACTACTAAGTCTGGTGATTGGTCGGCTAGCGTCAAGAAAGACTTTCCTCACCTTAGAGGAAACCGTAATGACA- CGCCAAGTGTCAAGTGGATTGGCGCTTACTCGCCTGT TACTTTCGAAGAGATTCCCTTGGGTAACAGGGATACAGTTAAGCAAGTGCTCTATGATTATGGATGGAAAGGT- GTTGAATTTAACGATACCGAGCAAGCGCATCTCGATG AGCATGGCGTATTACCCAAGCCTTGGAGTGGGAAGATAAATGAAAAGTCCCTTACTTTATGGCAAGAGAGAGC- CGCACGTGAAGGTAAAACAGTCCCTGATTGGTGCTT GGGTATCGCTGCATGGTACATACTCGTATCCCGTCGTGGTCAGATCCTCAACCGTGGTGACGTTGAAGCCTTC- GACCAGAAGGGGGTGTGGCCTTCGCAAGCTGGTATAC GAAAGTGTCGCGGCCTTGTACCTGTAGCATTTAACAAGGAGTTAGGAATCAATGCGCAGCAATACTACGAAAG- GTACGGATGCTGGCCTACGTCAGACAAGGATGACGG AGAATGGCGTGTGCCAGCTATTGCTATTAGTATTGGAACTTCTACGTTCCGTATGCGTCATCGTAACGTGGTT- AATATTCCTGCCCGTGGCTTGTATCCTTTACGTGATTTA TTCATAGCAGGGAAAGGCAAGCTAATCCTTGGTTGTGACGGTGCAGGTCTTGAACTGCGTGTCCTGTCTCACT- TCATGAATGACCCTGAGTACCAAGAGATTGTACTGCA CGGTGATATTCATACGCATAACCAGATGAAGGCTGGTCTTCCTAAGCGTGATATGGCGAAGACATTTATATAT- GCCTTCCTATATGGGTCTGGTATAGCTAACCTTGCAGC AGTATGTGGTGTTACTGAGGAAGAAATGGAGGAAGTTGTGGCAAGATTTGAGGTTGAACTACCATCTCTTGCA- CGTCTTCGTGAGAATGTTATCGCACAAGGTAACAAGT TTGGCTACCTACAAGCACCTGATGGTCATTGGGGTCGCATCCGTATGTCTGGTGGTGAACTTAAAGAACACAC- TATGCTTAACGTACTACTCCAGATGACTGGTTCTCTGT GTATGAAATACGCATTGGTCAGAGCGTTTGCAGTGATGCGCAAGGAAGGTGTGGCCTTAGATAGCATGGGAAA- CCCTTGCGGTATAGCTAACGTGCACGATGAAATCCA GATGGAAGTCCCTGAAGATGAGGTCTTGTATCTCAACTACGACTTGCCTTTCACCTTAGAAGGGTTCGAAACA- GAGAAGGCTGCTGTGAAAGCAGTGTTCGATGCAGAG GAGAAACGTGTTCATGTGGATTCTGAAGGACGTATGTGGTCTGCTGCAAATCTCGTTAGTGTTGATGCTGGTG-

TACTTCATTGCCAGCGTCGTTATCACCGTGCAGGGCAT ATCATTGCCGACGCAATGACCTGGGCGGGTCAGTACCTGAAGATGCGTTGTCCGATGGCAGGTGAGTATAAGA- TTGGTGCAAGTTGGAAGGAAACACACTGATGGACAG GTTTGATATTGTTTGCCTATTCTCTACCTTCTTTCTTATATTCCTTATGCTTGCTTGCTATGGAAGTATGCGA- TTAGATATACCTGATGAAGAGGAGGGTTACGATTGATGC AGGCATCTTTTATTATTCTTGGAGTCATATTATTTATGGTAGTATTCTGGGCTTTCTCTGGCATTGACCCAGA- TTGTGATGGTAACTACGACTGAGTTATACTCAAGGTCAC TTACGAGTGGCCTTTATGAATAACTTATTCCTACTTATTTTGTCTAACATGATTTACTGGACACTATAGAAGG- AAAGCATAGGTAATCTAGGTTTATAAGGTAGTATAGGT AATTAAGTAAATATAGGAGATATAAATATGTCTATGGTAACTACTCTGGTATTCGTGGCTCAATACTTTCGTG- GTCTTGCTAATAAGTTCAAGTCCAAGGCTATCAAAGCT ATTGAGGCTCGCATCGAAGCAGTACAGGCAGAGCAAGTTAAAGTTGAAGAACATCGTAGTTCTCAAATGATTG- ACTGTCATAACCGCTACTATGCATCTCGTGATGAACT AAATGCACGTCAAGTCAAAGAGGTAGAAGATATGCTGGCACGTCACCAGCAAGAGCGTGACAGCCTGAAAGCT- GAATTTGAAGAGAACAAGGCATCAATTGCTCTTGTA CATCAAGCTGCATCTGACAGTCTGAAGAAAGAGATTGTTATGCTGGAAATCGAACTGGATAACCTGACCAAAT- AAGGGGGGGTTATGATGGAAGAAGTAATTCAAGCTA AACATGTAGGTATTATCTTTCGCGATCTAGAGCAGCGTAAAGTTGCAGGTCATACTCGTCTGGCTAAAGAGGA- AGACACCGCAATCACTACTGTAGAACAAGCAGATGC CTATCGTGGACCAGAGTTCACTCAAGGTGAAACTTGTCACCAATTGAGCCTATCAATTTGTGACACTATGGCT- ATTGTAAATGTGCAAGAAGTCGAAGAGGGTGAGTGTG TCAGTTACATCTACCCTTTAGATACTATTGCACGCATTAAGGTAATCCATAAGTAATTACTAGACACTATAGA- ACAATAGGTCGGCTTAGTTCGGCCTATGATTGTAAAGT GTTGTTGATGTTGAACCATTGTGCATCTTGCACAACCCGATACCGTATAGGGCTTTCTAGTGAGTACATGCTT- GTGCTCAGTACAAAGCTAACTGACAATAGGAGACTAA ATAAATGGCACGTGGTGATTTTGATTTTGGTGCTCAGGTTACTAAATCTGAAGGTAAAGTCTTTAAGAATCCA- GAAGTAGGTGATCATGAAGCAGTAATCTCTGGCATCA TTCATGTTGGTTCCTTCCAAGACATCTTTAAGAAAGGTAATACCACTGAAGTTAAGAAGCCAGCAAACTTTGT- TCTGGTTAAGATTGTCCTGATGGGTGACGATGACAAG AACGAAGATGGTTCTCGCATGGAACAATGGATGGCTGTGCCTCTGAAGTCTGGTGATAAGGCAACACTGACTA- AGTTCCTGAATGCAGTTGACCCTAAAGAGTTGCTGG GTGGCTTCGATGATTTCATTGGTGAATGCCTGACTGCAACGATGGTCGGTTCTGGTGATAAGAATGACGATGG- CTCATTCAAGTATGTTAACTGGAAGGGATTTGGTGGT ATGCCGGACAAGCTGAAGAAACTGGTCATTGCTCAGGTTGAAGAGGAAGGTCTGTCTATGACAGGTCACATTA- CCTTCGACAAGCTGACCAAAGAAATCCTTGATGACA TCCCAGCCAACTTGGTGCGTCAATACTTCCTGAACGAGACGCCTCGTGGTAAGAACCTGTCTGTTGCTGGTTC- TCACGTAGAAGCAATCATTAAAGCTGCTCGTGAAGAA GACCCAGAATGGAAGAAGGCTAAGAAGAAAGACGAGGAAGATGCTACCCCAGCTAATCGTAAATCTCTGGATA- CTGGTGAGTCTGTTCCACAGGAAGTACCTGAAGCA GAAGATACTCCTGCACCGGAGATGGATGAGGACGCGGAATATTAAGGAGAAAGGATGAAAGTACAAATCGTAA- CCCTGCACTGCAAGAAAGGAATTACAACTCTTGGC GGCAACACTTTTCACTCCTTCTCTGAAGGGGACACATATGCCGACCTGCACTACATCTGGCGCGACGGACAGC- ACGTGGTGAACTACAGCGACCCAGCTACGGGGAAAC GCCACGGCGTATCGCTTCCGGCGCATGACATTGCTCAGGTGAACACAGTTTTATAAAGTCTCACGTGTGAGAC- AAATCGGTGTCCGGTATTTACTGGACACTATAGAAGA GAAGAATTTTAATCGGCGATAATGCCATAACCAACAAAAGGAGAATTTAATATGTTCAAGATTGAAACTATCG- TAAACCGTGTTGTTAAAGGTGCTGCTCTGGTATCCGT TGAGTCTTTCATTATCGTCGATGAAACTGATCAACTGGTAGCTGGTACTAAGGCTTACGATACCCGTGAAGAA- GCTCAGGCTAAGATTGACAGCATGGGTAACTTCGCTG CTGGTCTGGAGTTCGCTCGTGCTTGCTTCCCTGAGCAGGCTGACAAAGCTCAGATTGGTAAGGCTAATATCGT- AGCTGAATATCTGGATTGGGTTGCTGCTGGTAAACCA GTGAAAGAAGTTAAGGCTGCTGAAGAAGCTGAAGCTCCAGCAGAAGAAGTAGCTGCACCGGAAACTCCGGTAA- GTGAAGAGGAAGAATTTTGATAATAGCAGGTGTTG CCTCTGTTAGTCCTAGCTGACTATCACGCTCACCTCATCTAATGCCCTGTCTGCCTTAGTGTAGGCAGGGTCT- TTTGCGTAATAGTTATTGGAGAATGAATTATGCCGACT ATTGAATCTCGAATTGAACTGGACATTAGCTACAATGCAATCACCAGACAGTATATTGGGGTTGCCTATGATT- ACAAAACTGGTGAGAAGCTAGTGGAGGTGAGACAAT GGGATGACTATTGGTTAAGACAGAACCTCCATGATGCGGTGTCCTCCTTCCTGAAGGAGTGGCCTACATGCGA- CCAAACTTCGACTTCGGAGCTACAGTATCGGAAGACA ATAACCTGTTGCTGTGGCCAACTGAAGGTAATCGAATCGCTTTAATAGATGCTGATATGTTACCTTACATCAT- AGGGTATACAATCAGTGATATGACTTATGTACGAGCC ACAACTCGTGTTAAGTCAGGGCAAGTCCCCTCAATCAAAGATACACCTGAGTGTAAGCAAGCGTGTGACCGTG- TGAACTCCTTGCTTAACTCTTGGGTGTATGCAGCAGA ATGTGATGCAGCTAAGTTGTTCATGACGAAATCAGAAGCTAACTTCCGTGTCCGCCTAGCATTCACCAAGCCT- TATAAAGGTCAACGTAAGACCGAGAAGCCTCCATTCT TCTATGAATTGCGAGAGCATCTCTTAGAGGTTCACGGTGCAATCTTGGCAGATGGAGAGGAAGCAGATGACCT- CATGAGTATCGCACAATGGGACAGCCACCGCCGCTT CCAGCAAGATACAGGTAACGAGTTCCCTATCGGTAGTCCAGAGCATAAAGCATTCTCTGATACTTGCATCGTT- TCCTTGGATAAGGATTTGATGATTGTTCCCGGTTGGCA TCTACAGCCGGGTCAAGAGAAGAAATGGGTAGAGCCTATGGGTTGGCTTGAGCTACGCCGTAAGGCTAATGGG- CAAGTCAAAGATCTAAAAGGTGCTGGCCTCATGTTC CACTATGCACAGATGATTATCGGTGATGATATTGATAACTATGCTGGCATACCAGGTCGTGGTGCTAAATATG- CCTATGATCTTCTCAAAGATTGTAAGACAGAGAAAGA GTTGTACATGGCAGTGCTGGGTGCTTACAAGGCTAAGTTCGGGCATGGACAAGTTAAAATTAAGAATTACCGA- GGTGGTTATCGTATCGGCAAAGCCTTTGACCTAATGC TTGAGTGTGGTCGCTTATCTCACATGGCAAGATTCAAGGGTGATATATGGCGAGCCGATAAGAACCCAATCTT- GTGGGGAGATGATGCGGAATGGTTAGCAAATTAAAA TCATCGGAGGTGGCAGCTTATAAGAAGGAATTGCTAGATAAGCAAGGATGGAAATGCCCTCTGTGTGGCGGCA- GTCTCAAAGCTGTCACACCTGTAAACCGTGTACTTG ACCATGACCATGAGACAGGATTCTGCCGCGCTGTTGTATGCCGAGGCTGCAATGGTGCGGAAGGGAAGATTAA- GGGTGTTATCTCTGGTTATGGTAAGGCTGGTAACAA CCGTTACTTCCAGCTTCAATGGTTAGAGCGACTATATGAATACTGGAAGTTACATAGTACGCCTCAGACAGAT- AAGTTATATCACAAACATCAAACGGAGGCAGAGAAG CGCGAGGCTAAGAACCGTAAGGCACGCCTTGCTTATGCAAGAAAGAAGGAGGTTAAAGTTGGGTAAGCTGCGC- AGCTTGTACAAAGACTCCGAGGTACTTGATGCAATC GAGCAAGCTACCGACGAGAAAGGTAATGTTAACTACAATGAGATGGCACGTGTATTATCGTGTCATACTGTGG- GTAAGAAGATTACCCGCCAGTTGGCTCGATACTGGC ATGGTCAATTCAAGAAGACCAAGAAGAATGGTGATTACTACCAGACCCTTCTGCAAGAAGATAAGCGTATCAA- AGAAGAGCGTAAGCTCAGGACTCCTGACCGCTACGA GGATTTGGCTATTGTGCCATTGCCTGACTCGCCTCATCGAAGTGTACTGGTGATCCCTGATACTCATGCACCT- TATGAGCACCCAGATACCCTAGAGTTCCTTGCAGCCGT GGCAGCACGTTACCGTCCAGACACAGTGGTACACCTAGGAGATGAGGCAGACAAACATGCCCTGTCATTCCAC- GATTCGGACCCAAATCTGGATAGTGCTGGCATGGAG TTAGAGAAGGCTCGTATCTTCATGCACAAATTGCACAAGATGTTCCCTGTGATGCGCCTGTGTCACTCTAACC- ACGGCTCTATGCACTTCCGTAAGGCAAGCGCCAAAGG CATCCCTGTGCAATACCTGCGCACCTATCGTGAAGTCTTCTTCCCGCAGGGAGGTGGCGACCAGTGGGATTGG- CAACATACGCACGTCCTTGAGTTGCCGAATGGTGAAC AAGTGGCATTCAAGCATCAACCTGCTGGCTCTGTCCTAGCAGATGCAGCGCATGAGCGTATGAACCTTGTGTG- TGGTCACTTGCACGGTAAGATGTCTGTGGAGTACGCA CGTAATACACATGAACAGTATTGGGCTGTGCAAGGTGGCTGCTTAATTGATGAGTCATCCCGTGCATTTGCCT- ATGGTCGTGAGTCTAAATACAAGCCAGCATTAGGTTG TGTGGTCATTCTGGAGGGTGTGCCTCACATTGTCCCGATGCAAACCAATAGCGACAACCGTTGGATTGGCAAG- ATTTAGTTGACACTATAGAACAAAGGGCTAGGTAAG ACTTTATCGGCTGGCGTATCCAAATGATATTGCACTAGCCCTTGATTGTATAGTGAATGGAGGATTCAATATG- TCACACTATGAATGTAAGAAGTGTCATAAGCGTTATG ATTACTGTACTTGTGGTCAAGAGAAAACATCTTTTAAAGTTGGAGACAAGGTATTTCGTAATGAAAAAGATTC- GATTCCTTGGAATCAATACTGCAAAGAAGCTGGTATT GACCCTGATAGCCCTGTAACCATAGATGATATTGATGGCATTAACTTGTGCTTTCGTGAGGTGAGGGGTACAG- GTTGGGATTCCAAAAAATTCAAACTTGCATCTGATAA GTTAGACAACAATATGGTAATTAAGCCTAAGCACTACGAGTTCTTTGATGGCGTAGAGGCAATCACTATCATT- GCCCGCAGTATGACCGAGAAGCAATTCGCTGGCTATT GCATGGGTAATGCTTTGAAGTACCGTCTACGTGCAGGTAAGAAGTTCAACACTGAAGAAGACCTGAAGAAAGC- AGATTACTACAAAGAGTTATTCCAGAAGCATCGTCA CGAATGTATTGATGAGGATATTTGATATGAATATCTTTGAGTTCCTAGGTCTTCCAGAAGACCACCGCAATCA- CCCATTCATGCTGGTGAAGCATCGCGGTGAAGTTCCTG AGAAGAAATTAACTTTTCCATGTTATGCACAGGTGAAACGAGATGGTATCTTTTCTGCTGTTGTTGTTCGCAC- TGATGGTGTCGTTGGCATTTTTGGTCGCACTGGTAAGA AATTGGCAAACACTGAAGGACTCGAACAAGCCTTTGCTACCTTTCCGGTTGGCATTTATCTTGGTGAGCTTCA- GTCTATGGCCATTGATATCTACCTTGAGGCAATCTCTG GGGTTGTGAACCCCAATCGCACTGAGCCACTTGATTTCATAGGCCAGCAGATTAAAGACAACCTGTATATCGA- CTTCTTCGATATGTTAACTATTAAGGCATTCCATGATG GATTCACTGATGTTTCTTATCTCAAACGTTACGATGCTTTACATCGTCGTATCGGCGCTCATCTTAGCGGGTG- CAACGCTATCCTTCCTATCACTCCTTGCCATAATGAGCG AGAAGTTGAAGCGTTTGCGCAAGAGCAAATAGATGCAGGACGTGAGGGTGCTGTATTCAAACTGGACTGCGAT- TATGAAGCAGGACACAAAGGTTATCGTCAGACTAAA GAAGTCCGTAAGGTAACCTATGACCTTACTTGTATTGGCTTTGAAGAAGGTAAAGGCAAATACAAAGGTAAGG- TAGCTAACCTCATTTTCAAATGGAAAGGAGGCAAGA CAATCAAAGCTATGTTAGGTAAGGGGTGGACTCATGCAGATGCAGAGCAGATGTTCCACGACATTAAACATGG- TGGACGATTGAATGTCATTGGTAAAATCTTTGAAGT CAAAGGTCTTCAGGATTCAAGCAAGGGCAACATTCGTCTGCCCAAAGCGGGAGAATTAAGACATGACAAAGAT- GAACCAGATTTCTTTTGATAGCATGAAGGCAACTCG TGCAGTTGAGGTAGCAGAAGCTATCTTCGAAACTTTATCCTGTGGCATGGAAGTGCCATATACTTTACTTGCT- GATGCAGAAGAACTTGGTCTTTCTGTAGAAGCTATCCA AGAGAAGGTTGACGAATTATATGGTACAGACGAAGAAGAAACCGACGATTTCATTTGAAGGAATGGAGATGCT- TGAGATGATTCTCAAGCCTTCTTCTCCTAAGGTGAC TAAGACTCATGAAGAGTTAATCGTTGATGAAGTTAAGCGTTACATCATGGATTGTGTCAGAGCACAACTGGTG- GTCCAATGATACGTCCAGCCTCCTTCCTAGATATTCCT GAGATTATAAACCTTGGGAATAAATATGTGGAAGAGGAAGTCAAGGTTGTAGCCCACCACTCAGCCTCATGGA- ATGCAGAACAAAGTGCCATAACCTTTGTGCATCTCTT AATAGAGACCCACCACTCAGCCTCATGGAATGCAGAACAAAGTGCACATAACCTTTGTGCATCTCTTAGTAGA- GAAGATTTATCCCTATGGGTTGCTGTAGATGAAGGGC AGATTGTAGGGTTCCTGTGGGCTGGCTATCACGAGTTGGCCCCTTGGACACCTGTAAGAGTTGCCTCTGACAT- TCTCTTTTATATTATACCAGAGAGGCGAGGAACACTAC TTGGTATGCGTCTCATCAAAGCCCTAAAGCAATGGGCTAGTGATAATGAATGCTCTGAGGTTCGCCTGTCTAT- CGCCTCTGGTATTAATGAAGAACGTGTCGGACGTATG TATAAGCGACTTGGCTTTGAACCGTTTGGCACTGTGTATAACCTGAAGTTCTAAGGAGATAACATGGGTGTTG- TAAAGAAAGCATTTAAGGCTATCGGTCTTGCTCAAGA TGCACCACGTATTGAAGCCAAAGTCCCAGCACAGCAGCTTGAGCGTAAGCCTGAGACTGAAGCTGAAGATATT- CAAATTGGTGCAGGGGATGATGCTACTGCATCTGCA AAAGGTAAGCGTGGCCTTGTCCGTCCGGTAGCTTCTAGCTTGAAGGTGTAATATGAAACAGAGCATAGATTTG- GAGTATGGAGGTAAGCGGTCTAAGATACCTAAGCTA TGGGAGAAGTTCTCCAATAAACGTAGCTCTTTCCTTGATAGGGCGAAGCATTACTCCAAATTAACCTTGCCCT- ATCTGATGAATGACAAAGGTGATAACGAGACTTCGCA GAATGGATGGCAAGGTGTAGGTGCTCAGGCAACCAACCATCTAGCCAACAAGCTAGCGCAAGTACTATTCCCT- GCACAGCGTTCCTTCTTCCGTGTAGACTTAACTGCAC AAGGTGAGAAGGTTCTTAATCAGCGTGGCCTGAAGAAGACAGAGCTAGCTACCATCTTCGCTCAAGTGGAAAC- ACGGGCAATGAAAGAGTTAGAGCAACGTCAATTCCG GCCTGCTGTAGTAGAAGCATTTAAGCATCTTATTGTTGCTGGCAGCTGTATGCTATACAAGCCGAGCAAAGGT- GCAATCAGTGCTATCCCAATGCATCACTACGTAGTTA ACCGTGATACCAATGGCGACCTGTTAGACATTATCTTGCTACAAGAGAAAGCCTTACGTACCTTTGACCCAGC- TACACGTGCGGTAGTAGAGGTTGGCCTGAAAGGTAAG AAGTGCAAGGAAGATGACAGCGTTAAGCTGTACACACATGCTAAGTATCTTGGTGATGGATTTTGGGAACTCA- AGCAATCTGCTGATGATATCCCTGTGGGTAAGGTGA GTAAAATCAAATCAGAAAAGCTACCTTTCATCCCATTAACTTGGAAGCGAAGCTATGGTGAGGATTGGGGTCG- ACCTCTTGCAGAGGATTACTCCGGTGATTTATTCGTT ATCCAATTCTTATCTGAAGCGGTTGCCCGTGGTGCTGCGCTGATGGCAGATATCAAGTACCTGATTCGTCCTG- GTGCTCAAACTGATGTTGACCACTTTGTTAACTCTGGC ACTGGTGAGGTTGTCACTGGTGTAGAAGAAGACATCCATATTGTACAGTTAGGTAAGTACGCAGACCTCACAC- CTATTAGCGCGGTTCTAGAGGTATACACTCGCCGTAT CGGTGTTGTCTTCATGATGGAGACAATGACACGCCGTGACGCCGAACGTGTTACTGCTGTAGAAATCCAGCGA- GATGCGTTAGAGATTGAGCAGAACATGGGTGGTGTA TACTCCCTCTTTGCTACTACTATGCAATCGCCAGTAGCGATGTGGGGTCTGCTGGAGGCAGGGGAGTCCTTCA- CTAGTGACTTAGTGGACCCTGTGATTATCACAGGTATT GAAGCTTTAGGACGCATGGCTGAGTTGGATAAACTGGCTAACTTTGCTCAGTATATGTCACTGCCATTACAAT- GGCCTGAGCCTGTCCTAGCTGCTGTGAAATGGCCTGA CTATATGGATTGGGTGCGTGGTCAAATCTCTGCTGAACTGCCGTTCCTTAAATCGGCTGAAGAGATGGCACAA- GAACAGGAAGCACAGATGCAAGCACAGCAAGCACAG ATGCTTGAAGAAGGTGTGGCTAAGGCCGTGCCGGGTGTAATTCAACAAGAACTTAAGGAGGCGTAATGTCTTT- CTCATTTACTGAACCGTCAACCACTCACCCTACTGCT GAAGAGGGTCCGGTAGAAACCAAGGAGGTAACAACTGATGCTGCTACTACTGATGCTCCTGCTGACGCTGGCA- CTTCTGTACAAGATGACAATGCTGGTGCACAACCTA CTGAAGACACCGGAGGAGAAGCTTCTGGACAGCCTTCAGAAAAAGGAGACAATGGCGGAGAGAATGGTGAACC- TAAGCCAGATGATACCGCGACCGACACTGAGGAA GTGCAATACTTCTTCGGAGAACATGAAGTAACAGTAGACATCCCACAGGATGTAACTGACAGCCTTAAAGAGA- AAGGCATTGATGCCAAGCAGGTTGCCAAGGAACTCT ATTCCAAAGGTGGCAAGTTTGAACTGTCAGATGCAACCAAGCAGAAATTGTATGATGCTTTTGGCAAGTTTGC- GGTAGATGCTTACCTATCAGGTCTAAAGGCTCAAAAT GAAGCCTTCTTCCTGAAAGAAGCCAACGCAGCTAAAGAGTTGGAAGCAGCTAACACCCAACGCTTCTCTGATG- TTTCTAAGGAAATTGGTGGCGAAGAAGGTTGGTCCC GTCTTGAGGAGTGGGCACTTGAAGCGCTGTCTGATGACGAACTAATGGCATTCAATGCGGTGATGGAATCTGG- CAACCAGTACCTGCAACAATATGCTGTTCGTGAACTG GAGGGTCGTCGTAAGCAGGCACAGGGGGATGATAAGCCATCCCTGATTGAGCCATCAGCACCTGCTAAGGCTA- ATGAAGAGAATGGCCCACTGACGCGAGATCAGTAC GTTCAAGCAATCGCAACTCTTAGCCAGAAGTACGGCAATGACCGTAAAGCTATGGCAGAAGCTCAGGCTAAAC- TGGACGCCCGTCGCCGTGCTGGCATGGCTCGCGGTA TCTAATTCAGTATTTACTGGACACTATAGAAGGGAGAAAAGTTCTCCCTAGTTATCAATTTGATTTATAAGGA- GATTATAATACATGTCTACACCGAATACTCTGACTAAC GTTGCTGTATCTGCGTCCGGTGAGGTTGACAGCCTTCTCATTGAGAAGTTTAATGGTAAGGTCAATGAGCAGT- ACCTGAAAGGTGAGAACATTCTGTCCTACTTTGATGTA CAAACTGTTACTGGCACTAACACAGTGAGCAACAAATATTTGGGCGAAACTGAGTTGCAGGTGCTAGCACCGG- GTCAGTCCCCTAATGCCACCCCTACTCAGGCGGATA AAAACCAGTTGGTAATTGATACCACTGTCATTGCTCGTAACACTGTGGCTCACATCCACGATGTACAAGGTGA- CATCGATAGCCTGAAACCAAAACTGGCTATGAACCAA GCCAAGCAACTGAAACGTCTGGAAGACCAGATGGCAATTCAGCAGATGCTGTTAGGCGGTATTGCTAACACCA- AGGCCGAACGTAACAAGCCGCGTGTTAAAGGGCATG GCTTCTCTATCAACGTTAACGTAACTGAGAGTGAAGCACTGGCTAACCCTCAGTATGTTATGGCTGCGGTAGA- GTATGCTCTGGAGCAACAGCTTGAGCAGGAAGTGGAC ATCTCTGATGTAGCTATCATGATGCCGTGGAAGTTCTTCAATGCTTTGCGTGATGCAGACCGAATTGTAGATA- AGACTTACACTATCAGCCAGTCTGGTGCAACCATTAAT GGCTTCGTTCTCTCTTCTTATAACTGCCCTGTGATCCCGTCTAACCGATTCCCTACCTTCGCTCAGGATCAGG- CTCACCACCTGTTGTCTAATGAAGATAACGGCTATCGTT ATGACCCTATCGCAGAGATGAATGGTGCAGTTGCTGTTCTGTTCACTTCCGACGCACTGCTGGTGGGTCGTAC- CATTGAAGTGACTGGTGACATCTTCTATGAGAAGAAA GAGAAGACTTATTACATTGACACCTTCATGGCTGAGGGTGCAATCCCTGACCGTTGGGAAGCAGTGTCTGTAG- TTACCACTAAACGTGATGCAACTACTGGTGATGCTGG AGGTCCTGGTGATGATCACGCAACCGTACTGGCTCGTGCACAGCGTAAGGCTGTATATGTCAAAACCGAAGGT- GCTGCGGCTGCATTCTCTGCTGCCCCAGCAGGTATCC AAGCGGAAGACCTTGTAGCGGCGGTACGTGCTGTAATGGCAAATGACATTAAGCCGACTGCAATGAAACCTAC- TGAGTAACACCTATGCCCTATCTACCTTGCGTAGGTA GGGTTCTTTTTGTTAGGAGGATTCATGCCTGTAATTAGACAAACCAGTAAATTAGGACATATGATGGAAGATG- TGGCCTTCCAGATTATTGATAGTAAGCTGGAAGCGGT AAACTTGTGTATGCGAGCTATTGGTCGTGAGGGTGTGGATTCCCTCGACTCAGGGGACTTGGACGCAGAAGAT- GCAAGCAAAATGATCGACATCGTATCCCAGCGGTTCC AGTACAACAAAGGAGGTGGCTGGTGGTTCAATCGTGAACCAAACTGGCAACTTGCACCAGACACTAACGGTGA- AGTTAATTTACCTAACAACTGCCTAGCAGTATTGCA GTGTTATGCTTTAGGTGAAAAGAAAGTACCTATGACTATGCGAGCAGGTAAGCTCTACTCTACTTGGAGTCAC- ACCTTTGATATGCGTAAGCATGTTAATGCTAATGGTA TGATTCGTCTTACCTTACTCACCTTACTACCCTACGAGCATCTACCTACAAGTGTAATGCAGGCTATTGCCTA- TCAAGCTGCTGTAGAGTTTATTGTGTCTAAGGATGCAG ATCAGACTAAGCTAGCCACTGCGCAGCAGATAGCCACTCAGCTTCTTATGGATGTACAATCTGAGCAAATGTC- ACAGAAGCGATTAAACATGCTGGTACATAACCCTACT CAGCGTCAGTTTGGTATCATGGCTGGTGGCTCTCAGAATGTACCTGCTTACTCTCATTCACCTTATGAGAGTT- GGGCGCTCCGTCCGTGGGAGGATCGTTAATGGAAGTAC AAGGTTCATTAGGTAGACAAATCCAAGGGATTAGCCAGCAGCCGCCAGCGGTACGCTTGGATGGTCAGTGCAC- AGCTATGGTTAATATGATACCTGATGTAGTGAATGG TACTCAATCACGCATGGGTACAACTCATATTGCAAAGATACTTGATGCGGGGACTGATGACATGGCTACTCAT- CATTATCGCAGAGGTGATGGTGATGAAGAGTATTTCT TCACGTTGAAGAAAGGACAAGTTCCTGAGATATTTGATAAGTATGGGCGCAAATGTAATGTGACTTCACAAGA- TGCACCTATGACCTACCTCTCTGAGGTTGTTAATCCA AGGGAAGATGTGCAATTCATGACGATAGCTGATGTTACTTTCATGCTTAATCGTAGGAAAGTAGTTAAAGCTA- GTAGCAGGAAGTCACCTAAAGTTGGAAACAAAGCCA TTGTGTTTTGTGCGTATGGTCAATATGGTACATCTTATTCCATTGTAATTAATGGGGCCAACGCTGCTAGTTT- TAAAACACCGGATGGTGGAAGTGCAGACCATGTTGAAC AAATTCGAACTGAACGTATCACTTCTGAATTGTACTCTAAGTTGCAGCAATGGAGCGGTGTGAGTGACTATGA- AATACAAAGAGACGGTACTAGTATATTTATCGAGAGA CGGGATGGTGCTAGCTTTACAATAACAACCACCGATGGTGCAAAAGGTAAGGACTTAGTGGCTATCAAGAATA- AAGTTAGCTCTACTGACCTACTCCCTTCTCGTGCGCC TGCTGGTTATAAAGTACAAGTGTGGCCTACTGGCAGCAAACCTGAGTCTCGTTACTGGCTGCAAGCTGAGCCT- AAAGAGGGAAACCTTGTGTCTTGGAAAGAAACAATA GCTGCTGATGTATTACTTGGGTTTGATAAAGGCACAATGCCTTACATTATTGAACGTACAGATATCATCAACG- GCATAGCTCAATTCAAGATAAGACAAGGTGATTGGGA

AGATCGTAAAGTAGGGGATGACTTGACTAACCCTATGCCCTCTTTTATTGATGAGGAAGTACCCCAGACAATA- GGTGGAATGTTCATGGTGCAGAACCGCCTATGCTTTA CAGCAGGTGAAGCGGTTATTGCTTCTCGTACATCATACTTCTTCGATTTCTTTCGTTATACGGTTATCTCTGC- ATTGGCAACTGACCCCTTTGATATTTTCTCAGATGCTAG TGAAGTCTACCAGCTAAAACATGCAGTGACCTTAGATGGCGCTACCGTGTTGTTCTCTGATAAGTCACAATTC- ATACTGCCAGGCGATAAGCCTTTAGAGAAGTCAAATG CACTGCTTAAGCCTGTTACAACATTTGAAGTGAACAATAAAGTGAAGCCAGTAGTAACTGGTGAATCGGTAAT- GTTTGCCACTAATGATGGTTCTTACTCTGGTGTACGA GAGTTCTATACAGACTCTTATAGTGACACTAAGAAGGCACAAGCAATCACAAGTCATGTGAATAAACTCATCG- AAGGTAACATTACCAACATGGCAGCAAGCACCAATG TCAACAGGTTACTTGTCACTACCGATAAGTATCGTAACATAATCTACTGCTACGATTGGTTATGGCAAGGAAC- AGACCGTGTACAATCAGCATGGCATGTATGGAAGTGG CCTATAGGTACAAAGGTGCGAGGTATGTTTTATTCTGGTGAATTACTTTACCTGCTCCTTGAGCGAGGAGATG- GCGTGTATCTGGAGAAGATGGACATGGGTGATGCACT AACCTACGGTTTGAATGACCGCATCAGAATGGATAGGCAAGCAGAGTTAGTCTTCAAGCATTTCAAAGCAGAA- GATGAATGGGTATCTGAGCCGCTCCCTTGGGTTCCTA CTAACCCAGAACTTTTAGATTGCATCTTAATCGAGGGTTGGGATTCATATATTGGCGGCTCTTTCTTATTCAA- GTACAACCCTAGTGACAATACTTTGTCTACAACCTTTGA TATGTATGATGACAGCCATGTAAAAGCGAAGGTTATTGTTGGTCAGATTTACCCTCAAGAGTTTGAACCTACG- CCTGTGGTTATCAGAGACAATCAAGACCGTGTATCCT ACATTGATGTACCAGTTGTAGGATTGGTTCACCTTAATCTTGACATGTACCCCGATTTCTCCGTAGAAGTTAA- GAATGTGAAGAGTGGTAAAGTACGTAGAGTATTAGCG TCAAACCGTATAGGTGGTGCTCTCAATAATACAGTAGGCTATGTTGAACCGAGAGAAGGTGTCTTCAGATTTC- CACTGAGAGCTAAGAGCACGGATGTTGTTTATCGTAT TATTGTAGAGTCACCTCACACATTCCAGCTTCGTGATATTGAGTGGGAAGGGAGCTACAATCCAACCAAAAGG- AGGGTCTAATGGCTATAGGTTCAGCCGTTATGGCTGG TATGTCTTCTATTGGTAGCATGTTTGCAGGCAGTGGTGCAGCAGCCGCTGCTGGAGGTGCTGCCGCAGGTGGC- GGAGGTTTGCTAGGTTCACTAGGTGGATTCCTAAGTG GCTCTACTGCTGGTTTCTCTAATGCTGGCCTTCTTGGTGCTGGCCTTCAAGGGTTAGGCTTGATTGGTGATCT- ATTTGGTGGAAGTGATGAAGCCAAGGCGATGAAGAAAG CACAAGAAGAGCAATGGCGGCAGCAGCTTATTGCTACACAAGAGGCGTACAAGACAGTGGCAGACGCAGAACG- TTCTGCTGCTAAACAATATCATGCAGATGCAATCA GTAATCAGGCTTCACTGCTACAGCAGCGAGCACAGGTTGCATTACTTGCTGGGGCTACTGGTACTGGTGGTAA- TTCTGTGTCCTCTATGCTTAATGACTTAGCAGCAGATG GCGGCAGGAACCAGAGTACTATCATTGATAACTATGAGAATCAGAAGATTAATTTCACCAACCAGCTTAAGTC- TATCCAACGTGGTGGTCAGATGCAGATGCGTGAGTTT AAGAAGCCTTCTGCTATGAATACCTTGGTTAAAGGTATTCCAAGTCTGGCATCTGCCTATGTAACTGGTAGTA- AGTCTGGCAAGGCATTGGGTAAAGCCTTAACTGATTCT CGCACATATTCATCTGGAACAAGAGGTATTTAATGGCAATTGAGCGACAAGCAGTACAAGGTCTGCCACAAGT- GCAGGCCACTTCTCCTAATGTCATGACCTTTGCACCT CAACAAGTGGGAGGTGTGGAGGCTGGCGTGGCTTCTACCTCCGGTAGTAGGTTTATCGAAGACCTTATTCGTG- CAGCAAGCAGCGTGGCTGATGTTACCACTGGTATCCT TAATCAGAAGATTGAGGAAGATAAGGTTGTTCAAATGGAACGGGCATATAACGGATTAATGCCTTCTGAGGAT- GCAACTCGTGGTGGCGCTCGTGCTAACATGCTTGTCA AAGCTCAACTGCTAGCTAATGATGAAGCAGCACGAATGAAAGACATGGCTACTCGTTTCCAAGGAACGGATGA- CGAATGGACACAACTTATGGTTGACTCTCGTAATGA GATGCAGAATAAGCTGTTCCAGCAATACCCTGAGTTGCAAGGTGACAAAGATACTATGCGTATGGTCACTAAT- GTCTTCCAAGAACAGCAGCCTCAGATTTGGGCTACAC GAACCCAGCATAAACTTGACCGTGAACAAGCAGACCGTGAGGATACCTTTGACGGGCGAGTGGCTTCTACTTG- GGATTCTAATATTGACCCTGAAGCCTCTGGCTATGCT TTACAGGAACGAATCCGCGAAGGTCTTACTCAAGGATTACTACCTGAACAGATGTACAAGAAGTTAGTCCAGC- GAGCAATTTCACTTGCACAAGGCGGTGATGTTAGCA TGGCTGAAGCCCTGAAGTATGTGAAGGACGATAAGGGTGTTTCTGTTTATGCTAAGAATCCACAGCTTATCAC- AGCCATCACTAGTGGTAATGCAGTTTGGGCTAGGAAT AATGTAGCTGATGTAACTCGTATGTCTTTCGAAGTTAAAGAATCCTACCTTGCAGGTGATTTAACTGATGAAG- AATTGTTGGAACGAGCACAGCACATTAATAATCTGAC AGGTAACTCTGTCTTCTCTAATCCAGAACTAGAGGCACTGATGCGCCAACGGGCTAAGCAGAATGCAGAGCTA- GGTGCAATGCAGGATATGCGACGTGAGCTTTACTCC GACCGCCTGACTGGCTTCCAAGGTAAGACTGATAAAGAGAAGAAGGCTTACATTGATGTTATCAAACAGGATA- GCCAACTTTATGCAGACCAGCAAATCAAACAACGTG GCTTGGACCCTTACAGTCAAGAGGCTGAAGCTATTCGTGGTGCAGTGGAAGTGCAGCGCCTGCAATTCATGAA- CTCCAAAGGCTTAGTGGATGATACCTTTGAGTCTCGT ATCAAAGCCATGGAATCTATGCTATCGCCTGAGCACTTTGCCAAGGGCGAACCACAGGAGTTGATGACTATTC- GCCAGTTGTGGGAACAGTTACCAGAAGAGAGCCGAG GTGTCTTTGGTGACACGGTGAATGGCTACATGGATAACTACAACACTGCACTACAAATGGGAGAGACACCTTT- GCAGGCTGCAAGGTTTGCGCGTAAAGCACAGCAGAA ATTCTCTCGTACTGAGAAGGAAACCAAGAAGTTCAACTCAGCTATTGGAGATGCACTGGATGAGGTATCTGGT- GCTGGCTGGTTTGATGGTAAAACCGAAGTGTCAGACT TAGGTAAAGCTATTGCGGAAGAAGAGTTACGAGCTAAGGCCAATATGTTGTGGTCTAGTGGTATGCGTAACAT- GGATTCCATCAAGAAGGCTTTAATTACTTGGGGCAAT AAACGCTACACTCAATCAGAGGATGCAAAGACTTCCGGTGGCTATTTCATTAAAGGTGATTACACTTCTGCAT- CTGATATGCTTATGTCAGTTGGGAAAGGCGTAAACCC TACCGATGTACCTCTGGCGCTTGGTAGGTATGTAGAAACACAGATGCCAGAATTGAAGAAGGAGCTTCAAGAG- GGGGAAACTAAAGATGATATATACATTGATTACAAT GAACAGAAAGGTACTTTCGTGATTCGTGCTGGTGCAGCAGGTCGCCCTCTTTCTGGAGTAATCCCTGTAACCT- CTTTAGATACCACTTCACTACTAGATTCTGCCTATCAG AAGAAAGTAGAGGAACGAGATAAAGGCGAGTATGTTCACCCGTATCGTACAGATATTGGTGCACAAGAGCCTA- TGCCAGCTAAACCAACTGCCAAAGATATTGGTAAAT TTGGACTAGCTAACTTCCTCATGTCTTCTGCTTTTGCTTCTGGTGAGAATCTGCCTTCTAACTTCGAGATTAA- CTATCGAGGTAATATGCAACAATTCTATGACAAGCTAGC TATGGATGAGAATAAAGATAAAGTTGGCTTTAATAAGGCAACTGGAACCTTTACTCCATATAAAGACGCTCAC- GGTGAGTCTATCGGTTACGGTCATTTCTTAACGGAAG AAGAGAAGCGAAACGGGTATATTAAGATTGGCGATGAACTAGTTCCCTATCGAGGGTCTATGTCTCAGCTTAC- AGAGAGCAAGGCTCGCGCTCTTATGGAGCAAGATGC TAAGAAGCATGTGCCTCCTACTCGTGACTGGAAGATTCCGTTTGACCAGATGCACCCTGCACAGCAACGTGGC- TTGATGGATTTAAGCTACAATTTAGGTAAAGGTGGAA TCCAGAACTCACCGCGTGCTCTTGCTGCATTCAAAGCTGGTAAGCTTACGGAGGGCTTTATCGAAATGCTGGG- CACTGCATCAAGTGAAGGTAAGCGTATTCCTGGCCTA CTGAAGCGACGCGCTGAGGCATACAATATGGCATCTGCTGGTGGTGTGCCTAAGATTACCGAAGTGGAGACTC- GTGAAGATGGCTCCATGTGGGTTAGGTTTGGTGGAC CTATGCCAGCAGGTTCTGTCTCGGCATGGACTCATAAACGTATTGGCGCGGATGGTTGGTATCAGGTTTATGA- GGCTGCACCTACCAAGTTAGCTAAAGATTCTAAGGTA GGTAAAGTTAAGTTGTAGTACCTAACTCAAGGCTTGTCTCACATGTGAGACAGGTCTTTATGATAGGCACTAT- GGAGGAATTATGGAACAAGACATTAAGACTAATTGGG CTGGATATGTCCAGTCTACTCCTGAGCCGTTTTCTATTGAGGCGGCTCCGGTATCGGCTCCTACGATACGCCA- GCGTAATGAGTTACAAGAGCAAGTTCTTGAAGCTAAA GCTGACGCTGATATCTTAGGTGCTGTAGGTGCTGCCTTCCAGAATGAGTGGTTGGCATTCGGAGGCAAGCGGT- GGTATGACCGTGCCACTGCTGATTTCACACCTCAACC AGACTTTGAGATACAACCTGAGCAACGTGAAGCACTACGTTTCAAATATGGTACGGATATGATGCAGACAATC- ACTGAGGGTGTTCGTTCTGAGGATGAATTGAACTTCC GTATTCAGAATGCGGATGAAGACCTTGAGCGCAATAAGCGCATTGCTCAGGCTGGCTGGGTTGGCTCTGTGGC- GACGATTGGCGCTGCTGTGCTTGACCCTGTGGGATGG GTTGCCTCTATTCCAACCGGTGGTGCCGCTAAAGTTGGACTCGTAGGCCGTGCTGTGCGTGGCGCTATCGCCG- CTGGCGTGAGTAATGCCGCTATTGAATCCGTATTGGTC CAAGGTGACATGACTCGTGATTTAGATGACATTATGGTAGCACTGGGTTCCGGTATGGCTATGGGTGGCGTTA- TTGGCGCTGTAGCGCGTGGTAGGGCCACTAAGCTCAG TGAGCAAGGTGATGACAGGGCTGCTAGCATTGTGCGCAGTGCAGACGCAGGGGACCGCTATGTTCGTGCTGTT- GCCGATGACAGTATCGGTGCGATGCGTGTTAAGGGC GCAGAGGTTCTCACTGAGGGTGTATTCGATATCTCCAGTAAGAGTGAAGACCTACTGAAAACCTTGCAACGAG- AAGGTAATGCGATTGATATGACACCTCGCCGTTGGG CTGGAACTATGTCTGCCCTCGGTACTGTCGTGCACTCATCTAAAGATGCAAGTATCCGAGGCCTTGGTGCTCG- TCTGTTTGAATCCCCACAAGGTCTAGGTATGCAGAAG GCATCTGCTAGTCTTATGCAGAATACTAACTTAAATCGCCTGAAATCTGCTGATATGAACCGCTTCAATGATG- GGTTTGATTTGTGGCTTAAAGAGAATAATATCAATCCA GTAGCAGGGCATACCAACTCTCATTATGTACAGCAATACAATGAAAAGGTGTGGGAGGCAGTGCGTATTGGCA- TGGATGAGTCTACACCTAAATCTATCCGCATGGCTG CTGAGGGACAACAGGCTATGTACAGAGAGGCGCTGGCTTTACGTCAACGTTCTGGTGAAGCGGGATTTGAAAA- GGTAAAAGCCGACAACAAATATATGCCTGATATCTT TGATAGTATGAAAGCCAGACGTCAATTCGATATGCACGATAAAGAAGACATCATCGAACTTTTCTCTCGTGCC- TACCAGAATGGCGCTCGTAAGATTCCAAAGGAAGCA GCAGATGAGATTGCACGAGCACAGGTAAATCGCGTTGCTGATGCTACCTTAACTGGAAAGCTTAGTTTTGAAA- AGGCAATGTCAGGTCAGACTAAGGCAGAGTATGAAG CTATCATGCGTAAGGCAGGCTTCAGTGATGAAGAAATTGAAAAGATGATAGAAGCTCTGGATAACAAAGAAAC- CAGAGATAACATCTCTAACCGAGCTAAAATGAGTTT AGGATTAGATGTTACTCAAGAATACAATGGCATTCGTATGCGTGACTTCATGAATACCAACGTGGAAGAGCTA- ACAGATAACTATATGAAGGAAGCAGCAGGTGGCGCT GCATTGGCTCGCCAAGGCTTCTCTACCTATCAGGCTGCACTTAATGCAATTGACCTTGTAGAGCGAAATGCAC- GAAACGCGGCTAAGGATAGCAAGGCTAGTTTGGCATT AGATGAAGAGATTCGTCAGATGCGAGAAGGTCTTCGCCTGATTATGGGCAAGTCGATTGATGCAGACCCACAG- GCTATATCTACTAAGATGATGCGTCGTGGTCGTGATA TCACAGGTGTGCTTCGCTTAGGTCAAATGGGCTTCGCACAGCTAGGTGAACTTGCCAACTTTATGGGTGAATT- TGGTATTGCTGCAACTACTATGGCTTTAGGTAAGCAAT TCCGCTTCACCTCTAAGGCGTTGCGTAATGGCGATGGCTTCTTCCGAGATAAGAACTTAGCTGAGGTTGAGAG- AATGGTGGGGTACATTGGTGAGGATAACTGGCTAACA ACTAAGGGTGCACGTCCTGATGAATTTGGTGATGTAACCACAGTAAGAGGGATGATGGCTCACTTTGACCAAT- CCATGAACTCAATACGTCGTGCTCAAACCAACCTATC ACTCTTCCGCATGGCACAGGGTTCTCTGGAGCGAATGACTAATAGGCAAATAGCTTTGTCTTTCATTGACCAC- CTTGAAGGCAAGAAGATTATTCCTCAGAAGAAACTGG AGGAACTTGGTCTTACTCAGGAGTTCATGACTAACCTACAGAAGCACTATGATGCTAACTCTAAAGGTTCTGG- CTTGCTTGGCTTTGATACAATGCCTTATGCCATGGGTG AAACTTTAGCTAATGCTATTCGTCGTAAGTCAGGTCTAATCATCCAACGTAACTTCATTGGTGATGAAGGTAT- CTGGATGAACAAAGCACTAGGTAAGACATTTGCACAG CTTAAGTCATTCTCTCTTGTATCTGGTGAGAAGCAATTTGGTCGAGGGATTCGCCACGATAAAATTGGTCTTG- CTAAGAAGACAGCTTACGGGTTTGCTTTGGGTTCAATA GTGTATGCGGCAAAAGCCTATGTGAACTCTATTGGGCGAGAAGACCAAGATGAATATTTGGAAGAGAAGTTAT- CGCCTAAAGGGTTGGCCTTTGGTGCAATGGGTATGA TGAGTACAACTGCTGTATTTAGTCTAGGTGGAGATTTCTTAGGTGGCCTAGGTGTTCTACCTTCCGAACTCAT- TCAATCACGCTATGAAGCAGGTTTCCAAAGTAAGGGTC TGATTGACCAAATACCTCTGGTTGGCGTTGGTGCAGATGCAGTAAATCTGGCTAACTCAATCAAGAAGTATGC- AGAAGGTGACACAGAAGGTGTAGATATCGCTAAGCG AGCACTCCGTCTTGTGCCACTTACCAATATAATAGGTGTCCAAAACGCATTGCGTTATGGCTTAGATGAACTG- GAGGATTGATGAGTTATACTTTCACAGAACATACAGC CAATGGTACGCAAGTCACCTATCCTTTTAGCTTTGCTGGTAGGGATAAAGGTTATCTTCGTGCCTCAGATGTG- ATAGTGGAGTCTCTTCAAGGTAACACTTGGATTGAAGT TACATCTGGCTGGCAACTAACTGGCACGCACCAGATTACTTTTGATGTAGCACCAGTTGCAGGTTTGAAGTTC- CGTATTCGAAGGGAAGTACAAAAAGAATATCCATACG CTGAGTTTGACCGTGGTGTTACCTTGGATATGAAGTCTTTAAATGGTTCTTTCATTCATATACTGGAGATTAC- ACAGGAGTTACTTGACGGGTTTTATCCAGAAGGATACT TCATTAAACAGAATGTAAGCTGGGGCGGCAATAAGATTACTGATTTGGCTGATGGCACAAATCCGGGAGATGC- AGTAAATAAAGGGCAGCTTGATGCCATCGACAAGAA GCATACAGATTGGAACGCCAAACAGGACATTGAGATTGCTGGCCTTAAGGCTGGTATGACTTCTGGTATTGCG- CACAGAACTGTTCCTTGGTACACGATAGCCCAAGGTG GTGAGATTTCCGTAAAACCACCTTATGAATTTCAAGATGCACTAGTTTTCCTTAATGGGGTATTGCAGCACCA- AATTGTAGGCGCATACTCTATAAGCAACAACACTATC ACTTTCGCAGAGCCGCTTGTGGCTGGTACAGAGGTGTATGTGCTGATTGGTAGTCGTGTGGCTACATCTGAAC- CTAATATTCAGTTGGAGTTGAACTTTGACTTAGTAGAA GGCCAACAAGTAGTACAGATTGGCTCTGCATTTAAGTACATTGAGGTCTACCTTGATGGATTATTACAACCTA- AACTTGCTTATCAGGTAGACGGTGACATTGTTACTTTC TCAGAAAGAGTACCAGAATGCCGGATGACTGCTAAGATTATCACAGCATAAGGAGGTGGGATGATTAACTCCG- AACTGGTAGATAGTGGTGTGAAGCTTGCGCCACCTG CACTCATATCAGGTGGGTACTTCCTCGGTATCAGTTGGGATAATTGGGTGTTAATAGCAACATTCATTTATAC- CGTGTTGCAAATTGGGGACTGGTTTTATAATAAGTTCA AGATTTGGAGGGAGAAGCGTGAGCGTACACAATAAACATGCAGCTACAGAGGACGAGGTTGGCATTCTGCATG- GTGCTATTACCAAAATCTTCAATAAGAAAGCACAGG CAATACTGGACACTATAGAAGAAGACCCTGATGCAGCATTACATTTAGTGTCTGGTAAGGATATTGGTGCGAT- GTGTAAGTGGGTTCTTGATAACGGCATTACCGCCACA CCTGCTGCACAGCAGGAAGAGTCCAAGTTATCTAAGCGCCTCAAGGCTATCCGAGAGGCATCCAGTGGTAAGA- TAATTCAATTCACTAAGGAGGATTGATGGCTAAGGC AAGAGAATCACAAGCGGAGGCTCTTGCCAGATGGGAGATGCTACAGGAGTTACAGCAGACCTTTCCTTACACC- GCGGAAGGTTTGCTTCTCTTTGCAGATACAGTTATTC ATAACTTAATTGCAGGCAACCCTCATCTGATTCGTATGCAGGCGGATATCTTGAAGTTCCTATTTTACGGACA- CAAGTACCGCCTCATCGAAGCGCCTCGTGGTATCGCTA AGACAACACTATCAGCAATCTATACGGTATTCCGTATTATTCATGAACCGCATAAGCGTATCATGGTTGTGTC- CCAAAACGCCAAGCGAGCAGAGGAAATCGCAGGTTG GGTAGTTAAAATCTTCCGTGGCTTAGACTTTCTTGAGTTTATGCTGCCGGATATCTACGCTGGGGACCGTGCA- TCCGTTAAGGCGTTTGAGATTCATTACACCCTACGTGG TAGTGATAAGTCTCCTTCTGTATCCTGTTACTCAATCGAAGCAGGTATGCAGGGTGCTCGTGCTGATATTATT- CTAGCGGATGACGTAGAGTCGATGCAGAATGCTCGTAC GGCAGCGGGCCGTGCCTTGCTTGAGGAGCTGACTAAGGAGTTTGAATCTATCAACCAGTTTGGGGATATCATT- TACCTTGGTACACCTCAGAACGTAAACTCTATCTACA ACAACCTACCTGCTCGTGGTTACTCTGTTCGTATCTGGACTGCGCGTTACCCTTCAGTAGAGCAAGAGCAATG- TTATGGCGACTTCCTTGCACCTATGATTGTTCAAGATA TGAAGGACAACCCAGCACTTCGCTCAGGGTACGGGTTGGATGGTAATAGTGGTGCACCTTGTGCCCCTGAAAT- GTATGATGATGAAGTCCTGATTGAGAAGGAAATCTCT CAGGGTGCTGCTAAGTTCCAGCTTCAGTTCATGCTTAACACTCGCATGATGGATGCTGACAGATACCCATTAC- GCCTGAACAATCTAATCTTCACCTCGTTTGGTACAGAG GAAGTCCCTGTGATGCCTACGTGGAGTAATGATTCCATAAACATCATTGGTGATGCACCTAAGTATGGTAACA- AGCCTACGGATTTCATGTACAGACCTGTAGCTCGCCC ATATGAATGGGGTGCTGTCTCCCGCAAGATTATGTATATTGACCCTGCGGGTGGTGGTAAGAACGGAGATGAG- ACGGGTGTAGCCATCGTATTCCTGCACGGCACATTCA TTTATGTGTATCAGTGCTTTGGTGTACCTGGCGGATACCGAGAGTCGTCCCTGAATCGCATTGTGCAGGCCGC- AAAGCAGGCGGGTGTTAAAGAGGTATTCATTGAGAAG AACTTTGGTCATGGCGCGTTTGAGGCGGTAATTAAGCCGTACTTTGAACGAGAGTGGCCTGTAACTCTGGAAG- AGGATTACGCCACCGGACAGAAAGAGTTGCGTATCA TTGAGACGCTGGAGCCGCTCATGGCAGCCCATAGGCTTATCTTCAATGCAGAGATGGTGAAGTCAGACTTTGA- GTCGGTACAGCACTATCCGCTTGAACTACGCATGTCC TACAGTCTTTTCAATCAAATGTCGAACATAACGATTGAGAAGAACAGCCTCCGGCACGATGACCGCCTAGACG- CCCTGTATGGCGCTATACGGCAATTAACTTCTCAGAT AGACTATGACGAGGTTACACGGATTAATCGCCTCAGAGCGCAGGAGATGCGCGATTACATCCATGCTATGAAC- ACACCTCATCTACGCAGGGCAATGCTATATGGAGAT TACGGTACTGAGCGAAGAGTGACCAACACTTCCGTAGCGATGCAGCAGCGAGTTTACGGGCAGAACTACCGAA- ATAAATCGGCAAGCAGAAATACACTTTCTGCAAGGA TTTCAAGGACTTATTAATTACTGGACACTATAGAAGGAAGGCCCAGATAATAAGAGAAAATAATAGGTAATAT- ATATATAGGTTAACCTAGGTTATATAGGTATGCCTTA GTATGGGTGTACTCCTGTACACCCTATTCCTTACTACCTTACTATATTTACATAATAGGAGAGAGACAATGGC- TAATGATTATAGTAGTCAACCATTAACAGGTAAGTCTA AGAGAAAGCAGGTACAACCTGTAAGTGAAGAACTAATGCTTCCGGTGCTCAAAAAAGAGGAAGTTAGTAAGAA- AAGCAATGTTATTAATGATGCCACCAAATCAGGTA AACAGAAAGGGGCCATGGTGTGCCTTGAAGTGAAAGGTGGTGTATTGAAGATTGCTATCGCGGTTGATGGCAA- AGAAGATTCAGAGTGGAAGTTAGTAACAGTGGAACC AACTGTTAACCCAGTTTAAGATAAGGAGGAAGATTACATGGCTAAATATGGTACTACAGGTTCTGTTACTGGT- CAGGCTTTTCGAGTAAAAGCAGTACAAACTATTGCAA CGGCAATCCCGATGCCTGTTGTTAAAGAAGAAGACCTTAAGAGTAAAGACCACCCTATCAACATCAAACATTT- ATCAGGTAAACAGAAAGGTGCAATGGTTGCTCTTGA GAAAGGTGACACAACCTTACATATTGCTGTTGCACGTGGTAGTGAACCCACAGACCCTTGGGATGTAACTGGT- ATGGAAAAGGACGCTGTTACTCCAGCAGGGGTATAA TAATGCTTAATAAATACTTCAAGCGTAAAGAGTTTGCTTGCCGTTGTGGGTGCGGTACATCCACTGTTGATGC- TGAATTACTACAGGTAGTCACAGATGTGCGTGAGCAC TTTGGTTCTCCTGTAGTTATCACTTCGGGTCATCGCTGTGCTAAGCACAATGCCAATGTAGGTGGCGCTAAGA- ACTCCATGCATCTTACTGGTAAGGCTGCTGACATTAAA GTGTCTGGCATATTACCTTCTGAAGTGCATAAGTATCTTACTAGCAAATACCAAGGCAAGTATGGTATAGGTA- AGTATAACTCCTTCACTCACATCGATGTACGGGATGG TTGTGCGCGATGGTAAGATGTGTTGAATGGTGTGAGCGTATGGTTGCCCAAGCTGCCGAGGATGGCAACTATG- ATGACTGGAAGAACTACTCTGACTTGTTAGCTCAATG GAAAGGGAGATGCAATGAAAAAGCTGTTTAAGTCTAAGAAGGTTGTAGGTGCACTGGTTGCACTTGTTATTGC- TCTTGTTTCTGTAGGTCTTGGTGTAGACCTTGGCTCTG GCACGGAATCCTCTGTGACAGATGTGGTCTGCCAAGTGATCACCTGTGAATAAGTTTCTAGAAGTTCTGGCAG- GTCTTATTGGCCTGCTTGTCTCTGCTAAGAAGAAACA AGAAGAGAAGGAGGCACAAAGTGAAGCGAATCATGTTAGTGACAACCCTTCTGATTGGTTCGCTGACCACTTC- CGGGTGTCAGCAGGCGTTACCAGAGAAAGCAATGGT GAAACCTCTGAGGCCGACGCTGACGGCAGTTTACGAGGTAGACGATAAGGTCTGCTTTAGTAAGCCTGACGCT- ACAAAACTTGGTTTGTACATTCTCTCGCTAGAACGCG GATACAATTAATACATAGCTTTATGTATCAGTGTCTTACGATTTACTGGACACTATAGAAGAGGTAAGATAGC- GCCGTTCTTTTGAGCGGCCTATTACTAGCCAATCTTCA TAGGGAGGGTTGGAAAGTAATAGGAGATAGCATGGCTAAATTAACCAAACCTAATACTGAAGGAATCTTGCAT- AAAGGACAATCTTTGTATGAGTACCTTGATGCGAGA GTTTTAACATCAAAGCCGTTTGGTGCTGCAGGTGACGCCACTACTGATGATACGGAGGTTATAGCTGCTTCAT- TAAACTCTCAGAAAGCTGTCACAGTCTCAGATGGTGT ATTCTCTAGCTCTGGTATTAACAGTAATTACTGTAACTTAGACGGCAGGGGTAGTGGCGTGCTAAGTCACCGT- TCAAGTACAGGTAACTACTTAGTATTTAACAATCTACG TGCAGGTCGCTTAAGTAATATTACGGTAGAAAGTAATAAGGCGACTGATACAACTCAGGGACAGCAGGTATCC-

CTTGCTGGTGGAAGTGATGTTACTGTAAGTGACGTT AACTTCTCAAACGTTAAAGGTACTGGTTTCAGTTTAATCGCATACCCTAATGATGCGCCACCTGATGGACTTA- TGATTAAAGGCATTCGAGGTAGCTATTCCGGCTATGCT ACTAATAAGGCAGCCGGATGCGTACTTGCTGATTCCTCAGTTAACTCCCTCATAGATAACGTCATTGCTAAGA- ACTACCCTCAGTTCGGAGCAGTAGAGTTGAAAGGTAC AGCCAGTTACAACATAGTCAGTAATGTTATAGGGACAGATTGCCAGCATGTAACTTACAACGGCACTGAAGGG- CCAATAGCTCCTTCTAATAACCTTATCAAGGGGGTG ATGGCTAATAACCCTAAGTATGCAGCGGTTGTTGCAGGCAAAGGAAGTACGAACTTAATCTCAGACGTGCTCG- TAGATTACTCAACTTCTGATGCTAGGCAGGCTCATGG TGTTACAGTAGAGGGTTCTGATAACGTCATAAATAATGTGCTTATGTCAGGATGTGATGGTACTAACTCTTTA- GGACAAGGGCAGACTGCTACAATTGCACGCTTTATAG GTACAGCTAATAACAACTATGCGTCTGTATTTCCTAGCTACAGTGCTACAGGTGTTATTACTTTCGAATCCGG- CTCTACCCGTAACTTCGTAGAGGTAAAGCACCCTGGCA GGAGAAACGACCTTCTCAGTTCTGCTAGTACTATTGACGGTGCAGCTACTATTGACGGCACTAGTAATAGTAA- CGTAGTGCACGCACCTGCCTTAGGGCAGTACATAGGT AGTATGTCAGGTAGGTTCGAATGGCGGATTAAGTCCATGTCACTCCCTTCAGGCGTTCTTACTTCTGCTGATA- AGTACAGAATGCTTGGAGATGGTGCTGTGTCATTAGCT GTAGGTGGGGGCACTTCTTCTCAAGTTCGCCTATTTACTTCTGATGGTACTTCTCGGACAGTGTCCCTCACCA- ACGGTAACGTGCGTCTTTCTACCAGTAGCACAGGCTTTT TGCAGTTAGGTGCTGATGCAATGACCCCAGACAGTACTGGTACATACGCATTAGGTTCCGCCAGCCGAGCATG- GTCTGGCGGTTTTACTCAAGCAGCATTCACTGTTACC TCAGATGCTCGGTGTAAAACAGAACCTCTTACTATCTCAGATGCCTTACTGGATGCTTGGTCTGAAGTTGACT- TTGTGCAGTTTCAGTATTTGGATCGTGTTGAGGAGAAG GGTGCAGACTCAGCTAGATGGCACTTCGGTATCATCGCTCAGCGAGCTAAGGAGGCTTTCGAACGTCACGGTA- TAGATGCACATCGCTATGGCTTCTTGTGCTTCGACAG TTGGGATGATGTATACGAGGAAGATGCCAATGGCTCTCGTAAACTGATTACACCAGCAGGTTCCCGCTACGGT- ATTCGTTACGAGGAAGTACTGATATTAGAGGCTGCGT TGATGCGGCGGACTATTAAGCGTATGCAGGAAGCACTAGCTTCCCTGCCTAAGTAAGCAACAGGCAGTGCGTA- AGCACTGCTTTTAGCGCAACTTTTCTTAAAGGTTATC ACGGTGGTAGCCTTTCAGAAAAGGAGGTTACATGATTCAAAGACTAGGTTCTTCATTAGTTAAATTCAAGAGT- AAAATAGCAGGTGCAATCTGGCGTAACTTGGATGACA AGCTCACCGAGGTTGTATCGCTTAAAGATTTTGGAGCCAAAGGTGATGGTAAGACAAACGACCAAGATGCAGT- AAATGCAGCGATGGCTTCAGGTAAGAGAATTGACGG TGCTGGTGCTACTTACAAAGTATCATCTTTACCTGATATGGAGCGATTCTATAACACCCGCTTCGTATGGGAA- CGTTTAGCAGGTCAACCTCTTTACTATGTGAGTAAAGG TTTTATCAATGGTGAACTATATAAAATCACGGATAACCCTTATTACAATGCTTGGCCTCAAGACAAAGCGTTT- GTATATGAGAACGTGATATATGCACCTTACATGGGTA GTGACCGTCATGGTGTTAGTCGTCTGCATGTATCATGGGTTAAGTCTGGTGACGATGGTCAAACATGGTCTAC- TCCAGAGTGGTTAACTGATCTGCATCCAGATTACCCTA CAGTGAACTATCATTGTATGAGTATGGGTGTATGTCGCAACCGTCTGTTTGCCATGATTGAAACACGTACTTT- AGCCAAGAACAAACTAACCAATTGTGCATTGTGGGAT CGCCCTATGTCTCGTAGTCTGCATCTTACTGGTGGTATCACTAAGGCTGCAAATCAGCAATATGCAACAATAC- ATGTACCAGATCACGGACTATTCGTGGGCGATTTTGTT AACTTCTCTAATTCTGCGGTAACAGGTGTATCAGGTGATATGACTGTTGCAACGGTAATAGATAAGGACAACT- TCACGGTTCTTACACCTAACCAGCAGACTTCAGATTT GAATAACGCTGGAAAGAGTTGGCACATGGGTACTTCTTTCCATAAGTCTCCATGGCGTAAGACAGATCTTGGT- CTAATCCCTAGTGTCACAGAGGTGCATAGCTTTGCTA CTATTGATAACAATGGCTTTGTTATGGGCTATCATCAAGGTGATGTAGCTCCACGAGAAGTTGGTCTTTTCTA- CTTCCCTGATGCTTTCAATAGCCCATCTAATTATGTTCG TCGTCAGATACCATCTGAGTATGAACCAGATGCGTCAGAGCCATGCATCAAGTACTATGACGGTGTATTATAC- CTTATCACTCGTGGCACTCTTGGTGACAGACTTGGAA GCTCTTTGCATCGTAGTAGAGATATAGGTCAGACTTGGGAGTCACTGAGATTTCCACATAATGTTCATCATAC- TACCCTACCTTTTGCTAAAGTAGGAGATGACCTTATTA TGTTTGGTTCAGAACGTGCAGAAAATGAATGGGAAGCAGGTGCACCAGATGATCGTTACAAGGCATCTTATCC- TCGTACCTTCTATGCACGATTGAATGTAAACAATTGG AATGCAGATGATATTGAATGGGTTAACATCACAGACCAAATCTATCAAGGTGACATTGTGAACTCTAGTGTAG- GTGTAGGTTCGGTAGTAGTTAAAGACAGCTACATTTA CTATATCTTTGGTGGCGAAAACCATTTCAACCCAATGACTTATGGTGACAACAAAGGTAAAGACCCATTTAAA- GGTCATGGACACCCTACTGATATATACTGCTATAAGA TGCAGATTGCAAATGACAATCGTGTATCTCGTAAGTTTACATATGGTGCAACTCCGGGTCAAGCTATACCTAC- TTTCATGGGTACTGATGGAATACGAAATATCCCTGCA CCTTTGTATTTCTCAGATAACATTGTTACAGAGGATACTAAAGTTGGACACTTAACACTTAAAGCAAGCACAA- GTTCCAATATACGATCTGAAGTGCAGATGGAAGGTGA ATATGGCTTTATTGGCAAGTCTGTTCCAAAGGACAACCCAACTGGTCAACGTTTGATTATTTGTGGTGGAGAA- GAGACTTCGTCCTCTTCAGGTGCACAGATAACTTTGCA CGGCTCTAATTCAAGTAAGGCTAATCGTATCACTTATAACGGAAATGAGCACCTATTCCAAGGTGCACCAATC- ATGCCTGCTGTAGATAACCAGTTTGCTGCTGGTGGAC CTAGTAACCGATTCACTACCATCTACCTAGGTAGTGACCCTGTTACAACTTCAGATGCTGACCACAAGTACAG- TATCTCTAGTATTAATACCAAGGTGTTAAAGGCTTGG AGCAGGGTTGGTTTTAAACAGTATGGTTTGAATAGTGAAGCAGAGAGGGACCTTGATAGCATACACTTCGGTG- TCTTGGCTCAGGATATTGTAGCTGCTTTTGAAGCTGA AGGGTTGGATGCCATTAAGTATGGAATTGTGTCCTTCGAAGAAGGTAGGTACGGTGTGAGGTATAGTGAAGTT- CTAATACTAGAGGCTGCTTATACTCGTTATCGTTTAG ACAAGTTAGAGGAGATGTATGCCACTAATAAAATCAGTTAAGCAAGCTGCTGTACTCCAGAACACAGAAGAGC- TTATTCAATCAGGACGTGACCCTAAGCAGGCTTATG CCATTGCCAAGGATGTTCAACGTCGTGCCATGAAGAAACCTTCTGCATCTTCTGCGTAAGCAGGTTAATATCT- TAGTATAAACAAGGGCAGACTTAGGTTTGTCCTTAGTG TATTCCAAAGGAGGTAACATGCTGAAAGATGGTTGGGTTTCATATGACCCTACAGACCCTAAGAATTGGCTAC- AGGTTATCGCTATAGCTTGTGCAGGTAGCCTATTGGC TGCCCTGATGTATTCATTATGGATGTACACAAAGTAACCAAAGTCAAAATTTTGATGTAGGCGTGTGTCAGCT- CTCTCGCCCTCGCCCTCGCCGGGTTGTCCCCATAGGGT GGCCTGAGGGAATCCGTCTTCGACGGGCAGGGCTGATGTACTCCTTGTCTAGTACAAGGGAGGCGGAGGGAAC- GCCTAGGGAGGCCTAGGAATGGCTTAGTGGTGGACA AGGTGATTACCTTAGTGAAGCCTCTTAGTGCATTCCTGAGGCCATTCAGGGCGTTTATGAGGGATTGACAGGG- TGTGAGGGCGTGGGCTA SEQ ID NO: 34-YAC pRS415 TGAGGTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA- TTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTG CGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTAT- CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT- TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC- CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG- TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAAC- CCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGG- CTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTG CTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG- GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAA AAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAA- GGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA CCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAG- TTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGA TCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACC- ATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCAC GCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAAC- TTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGG GAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGT- CACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT TCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGA- TCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACT CATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAG- TACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCG ACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATC- ATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTT- CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAA ATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTG- AAGCATTTATCAGGGTTATTGTCTCATGAGCGGATAC ATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGGGT- CCTTTTCATCACGTGCTATAAAAATAATTATAATTTA AATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTT- TTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAA CAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAG- AAGACCACACACGAAAATCCTGTGATTTTACATTTTACT TATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTT- TGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCA TTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAG- AGTAAATTCCCAAATTATTCCATCATTAAAAGATACGA GGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATA- GGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGG TGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGG- AGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTT GGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATCGACTACG- TCGTAAGGCCGTTTCTGACAGAGTAAAATTCTTGAGG GAACTTTCACCATTATGGGAAATGGTTCAAGAAGGTATTGACTTAAACTCCATCAAATGGTCAGGTCATTGAG- TGTTTTTTATTTGTTGTATTTTTTTTTTTTTAGAGAAAA TCCTCCAATATCAAATTAGGAATCGTAGTTTCATGATTTTCTGTTACACCTAACTTTTTGTGTGGTGCCCTCC- TCCTTGTCAATATTAATGTTAAAGTGCAATTCTTTTTCCT TATCACGTTGAGCCATTAGTATCAATTTGCTTACCTGTATTCCTTTACTATCCTCCTTTTTCTCCTTCTTGAT- AAATGTATGTAGATTGCGTATATAGTTTCGTCTACCCTAT GAACATATTCCATTTTGTAATTTCGTGTCGTTTCTATTATGAATTTCATTTATAAAGTTTATGTACAAATATC- ATAAAAAAAGAGAATCTTTTTAAGCAAGGATTTTCTTAA CTTCTTCGGCGACAGCATCACCGACTTCGGTGGTACTGTTGGAACCACCTAAATCACCAGTTCTGATACCTGC- ATCCAAAACCTTTTTAACTGCATCTTCAATGGCCTTAC CTTCTTCAGGCAAGTTCAATGACAATTTCAACATCATTGCAGCAGACAAGATAGTGGCGATAGGGTCAACCTT- ATTCTTTGGCAAATCTGGAGCAGAACCGTGGCATGGT TCGTACAAACCAAATGCGGTGTTCTTGTCTGGCAAAGAGGCCAAGGACGCAGATGGCAACAAACCCAAGGAAC- CTGGGATAACGGAGGCTTCATCGGAGATGATATCAC CAAACATGTTGCTGGTGATTATAATACCATTTAGGTGGGTTGGGTTCTTAACTAGGATCATGGCGGCAGAATC- AATCAATTGATGTTGAACCTTCAATGTAGGGAATTCG TTCTTGATGGTTTCCTCCACAGTTTTTCTCCATAATCTTGAAGAGGCCAAAACATTAGCTTTATCCAAGGACC- AAATAGGCAATGGTGGCTCATGTTGTAGGGCCATGAAA GCGGCCATTCTTGTGATTCTTTGCACTTCTGGAACGGTGTATTGTTCACTATCCCAAGCGACACCATCACCAT- CGTCTTCCTTTCTCTTACCAAAGTAAATACCTCCCACTA ATTCTCTGACAACAACGAAGTCAGTACCTTTAGCAAATTGTGGCTTGATTGGAGATAAGTCTAAAAGAGAGTC- GGATGCAAAGTTACATGGTCTTAAGTTGGCGTACAAT TGAAGTTCTTTACGGATTTTTAGTAAACCTTGTTCAGGTCTAACACTACCGGTACCCCATTTAGGACCACCCA- CAGCACCTAACAAAACGGCATCAACCTTCTTGGAGGCT TCCAGCGCCTCATCTGGAAGTGGGACACCTGTAGCATCGATAGCAGCACCACCAATTAAATGATTTTCGAAAT- CGAACTTGACATTGGAACGAACATCAGAAATAGCTTT AAGAACCTTAATGGCTTCGGCTGTGATTTCTTGACCAACGTGGTCACCTGGCAAAACGACGATCTTCTTAGGG- GCAGACATAGGGGCAGACATTAGAATGGTATATCCTT GAAATATATATATATATTGCTGAAATGTAAAAGGTAAGAAAAGTTAGAAAGTAAGACGATTGCTAACCACCTA- TTGGAAAAAACAATAGGTCCTTAAATAATATTGTCA ACTTCAAGTATTGTGATGCAAGCATTTAGTCATGAACGCTTCTCTATTCTATATGAAAAGCCGGTTCCGGCCT- CTCACCTTTCCTTTTTCTCCCAATTTTTCAGTTGAAAAA GGTATATGCGTCAGGCGACCTCTGAAATTAACAAAAAATTTCCAGTCATCGAATTTGATTCTGTGCGATAGCG- CCCCTGTGTGTTCTCGTTATGTTGAGGAAAAAAATAA TGGTTGCTAAGAGATTCGAACTCTTGCATCTTACGATACCTGAGTATTCCCACAGTTAACTGCGGTCAAGATA- TTTCTTGAATCAGGCGCCTTAGACCGCTCGGCCAAACA ACCAATTACTTGTTGAGAAATAGAGTATAATTATCCTATAAATATAACGTTTTTGAACACACATGAACAAGGA- AGTACAGGACAATTGATTTTGAAGAGAATGTGGATTT TGATGTAATTGTTGGGATTCCATTTTTAATAAGGCAATAATATTAGGTATGTGGATATACTAGAAGTTCTCCT- CGACCGTCGATATGCGGTGTGAAATACCGCACAGATGC GTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTA- AATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGC AAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCAC- TATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAA AAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCG- TAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCG ATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCT- AGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCG TAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAA- CTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTA TTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC- GACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAAT ACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGA- ATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCG GCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGG- TCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC AATTCCACACAACATAGGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG

[0159] The Sequence Listing in the accompanying text file entitled "Sequence Listing" (created on Sep. 4, 2014 and having a size of 187 KB) is incorporated by reference herein.

[0160] While several inventive embodiments have been described and illustrated herein, those of ordinary skill in the art will readily envision a variety of other means and/or structures for performing the function and/or obtaining the results and/or one or more of the advantages described herein, and each of such variations and/or modifications is deemed to be within the scope of the inventive embodiments described herein. In addition, any combination of two or more such features, systems, articles, materials, kits, and/or methods, if such features, systems, articles, materials, kits, and/or methods are not mutually inconsistent, is included within the inventive scope of the present disclosure.

[0161] All definitions, as defined and used herein, should be understood to control over dictionary definitions, definitions in documents incorporated by reference, and/or ordinary meanings of the defined terms.

[0162] All references, patents and patent applications disclosed herein are incorporated by reference with respect to the subject matter for which each is cited, which in some cases may encompass the entirety of the document.

[0163] The indefinite articles "a" and "an," as used herein in the specification and in the claims, unless clearly indicated to the contrary, should be understood to mean "at least one."

[0164] As used herein in the specification and in the claims, the phrase "at least one," in reference to a list of one or more elements, should be understood to mean at least one element selected from any one or more of the elements in the list of elements, but not necessarily including at least one of each and every element specifically listed within the list of elements and not excluding any combinations of elements in the list of elements. This definition also allows that elements may optionally be present other than the elements specifically identified within the list of elements to which the phrase "at least one" refers, whether related or unrelated to those elements specifically identified.

[0165] It should also be understood that, unless clearly indicated to the contrary, in any methods claimed herein that include more than one step or act, the order of the steps or acts of the method is not necessarily limited to the order in which the steps or acts of the method are recited.

[0166] In the claims, as well as in the specification above, all transitional phrases such as "comprising," "including," "carrying," "having," "containing," "involving," "holding," "composed of," and the like are to be understood to be open-ended, i.e., to mean including but not limited to. Only the transitional phrases "consisting of" and "consisting essentially of" shall be closed or semi-closed transitional phrases, respectively, as set forth in the United States Patent Office Manual of Patent Examining Procedures, Section 2111.03.

Sequence CWU 1

1

90139937DNAArtificial SequenceSynthetic Polynucleotide 1tctcacagtg tacggaccta aagttccccc atagggggta cctaaagccc agccaatcac 60ctaaagtcaa ccttcggttg accttgaggg ttccctaagg gttggggatg acccttgggt 120ttgtctttgg gtgttacctt gagtgtctct ctgtgtccct atctgttaca gtctcctaaa 180gtatcctcct aaagtcacct cctaacgtcc atcctaaagc caacacctaa agcctacacc 240taaagaccca tcaagtcaac gcctatctta aagtttaaac ataaagacca gacctaaaga 300ccagacctaa agacactaca taaagaccag acctaaagac gccttgttgt tagccataaa 360gtgataacct ttaatcattg tctttattaa tacaactcac tataaggaga gacaacttaa 420agagacttaa aagattaatt taaaatttat caaaaagagt attgacttaa agtctaacct 480ataggatact tacagccatc gagagggaca cggcgaatag ccatcccaat cgacaccggg 540gtcaaccgga taagtagaca gcctgataag tcgcacgaaa aacaggtatt gacaacatga 600agtaacatgc agtaagatac aaatcgctag gtaacactag cagcgtcaac cgggcgcaca 660gtgccttcta ggtgacttaa gcgcaccacg gcacataagg tgaaacaaaa cggttgacaa 720catgaagtaa acacggtacg atgtaccaca tgaaacgaca gtgagtcacc acactgaaag 780gtgatgcggt ctaacgaaac ctgacctaag acgctcttta acaatctggt aaatagctct 840tgagtgcatg actagcggat aactcaaggg tatcgcaagg tgccctttat gatattcact 900aataactgca cgaggtaaca caagatggct atgtctaaca tgacttacaa caacgttttc 960gaccacgctt acgaaatgct gaaagaaaac atccgttatg atgacatccg tgacactgat 1020gacctgcacg atgctattca catggctgcc gataatgcag ttccgcacta ctacgctgac 1080atctttagcg taatggcaag tgagggcatt gaccttgagt tcgaagactc tggtctgatg 1140cctgacacca aggacgtaat ccgcatcctg caagcgcgta tctatgagca attaacgatt 1200gacctctggg aagacgcaga agacttgctc aatgaatact tggaggaagt cgaggagtac 1260gaggaggatg aagagtaatg tctactacca acgtgcaata cggtctgacc gctcaaactg 1320tacttttcta tagcgacatg gtgcgctgtg gctttaactg gtcactcgca atggcacagc 1380tcaaagaact gtacgaaaac aacaaggcaa tagctttaga atctgctgag tgatagactc 1440aaggtcgctc ctagcgagtg gcctttatga ttatcacttt acttatgagg gagtaatgta 1500tatgcttact atcggtctac tcaccgctct aggtctagct gtaggtgcat cctttgggaa 1560ggctttaggt gtagctgtag gttcctactt taccgcttgc atcatcatag gaatcatcaa 1620aggggcacta cgcaaatgat gaagcactac gttatgccaa tccacacgtc caacggggca 1680accgtatgta cacctgatgg gttcgcaatg aaacaacgaa tcgaacgcct taagcgtgaa 1740ctccgcatta accgcaagat taacaagata ggttccggct atgacagaac gcactgatgg 1800cttaaagaaa ggttatatgc ccaatggcac actatacgct gcaaatcggc gaatagtgag 1860aacttggcga gagaacaacc tcgaacgccg caaggacaag agagggcggc gtggcataga 1920cgaaaggaaa aggttaaagc caagaaactc gccgcacttg aacaggcact agccaacaca 1980ctgaacgcta tctcataacg aacataaagg acacaatgca atgaacatta ccgacatcat 2040gaacgctatc gacgcaatca aagcactgcc aatctgtgaa cttgacaagc gtcaaggtat 2100gcttatcgac ttactggtcg agatggtcaa cagcgagacg tgtgatggcg agctaaccga 2160actaaatcag gcacttgagc atcaagattg gtggactacc ttgaagtgtc tcacggctga 2220cgcagggttc aagatgctcg gtaatggtca cttctcggct gcttatagtc acccgctgct 2280acctaacaga gtgattaagg tgggctttaa gaaagaggat tcaggcgcag cctataccgc 2340attctgccgc atgtatcagg gtcgtcctgg tatccctaac gtctacgatg tacagcgcca 2400cgctggatgc tatacggtgg tacttgacgc acttaaggat tgcgagcgtt tcaacaatga 2460tgcccattat aaatacgctg agattgcaag cgacatcatt gattgcaatt cggatgagca 2520tgatgagtta actggatggg atggtgagtt tgttgaaact tgtaaactaa tccgcaagtt 2580ctttgagggc atcgcctcat tcgacatgca tagcgggaac atcatgttct caaatggaga 2640cgtaccatac atcaccgacc cggtatcatt ctcgcagaag aaagacggtg gcgcattcag 2700catcgaccct gaggaactca tcaaggaagt cgaggaagtc gcacgacaga aagaaattga 2760ccgcgctaag gcccgtaaag aacgtcacga ggggcgctta gaggcacgca gattcaaacg 2820tcgcaaccgc aaggcacgta aagcacacaa agctaagcgc gaaagaatgc ttgctgcgtg 2880gcgatgggct gaacgtcaag aacggcgtaa ccatgaggta gctgtagatg tactaggaag 2940aaccaataac gctatgctct gggtcaacat gttctctggg gactttaagg cgcttgagga 3000acgaatcgcg ctgcactggc gtaatgctga ccggatggct atcgctaatg gtcttacgct 3060caacattgat aagcaacttg acgcaatgtt aatgggctga tagtcttatc ttacaggtca 3120tctgcgggtg gcctgaatag gtacgattta ctaactggaa gaggcactaa atgaacacga 3180ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg ttcaacactc 3240tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag catgagtctt 3300acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg 3360ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag atgattgcac 3420gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc 3480agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag accactctgg 3540cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca atcggtcggg 3600ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag cacttcaaga 3660aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc 3720aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt 3780ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc attgagtcaa 3840ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac tctgagacta 3900tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca 3960tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc attactggtg 4020gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag 4080cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt aacattgcgc 4140aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta atcaccaagt 4200ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc ccgatgaaac 4260cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg 4320tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc atgcttgagc 4380aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg gactggcgcg 4440gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc 4500ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg aaaatccacg 4560gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag ttcattgagg 4620aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact tggtgggctg 4680agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg gtacagcacc 4740acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc tctggcatcc 4800agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac ttgcttccta 4860gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag attctacaag 4920cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag aacactggtg 4980aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg ctggcttacg 5040gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg tccaaagagt 5100tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat tccggcaagg 5160gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg atttgggaat 5220ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag tctgctgcta 5280agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc aagcgttgcg 5340ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag aagcctattc 5400agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc attaacacca 5460acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct aactttgtac 5520acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa 5580tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac gctgcgaacc 5640tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat gtactggctg 5700atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa atgccagcac 5760ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc gcgttcgcgt 5820aacgccaaat caatacgact cactatagag ggacaaactc aaggtcattc gcaagagtgg 5880cctttatgat tgaccttctt ccggttaata cgactcacta taggagaacc ttaaggttta 5940actttaagac ccttaagtgt taattagaga tttaaattaa agaattacta agagaggact 6000ttaagtatgc gtaacttcga aaagatgacc aaacgttcta accgtaatgc tcgtgacttc 6060gaggcaacca aaggtcgcaa gttgaataag actaagcgtg accgctctca caagcgtagc 6120tgggagggtc agtaagatgg gacgtttata tagtggtaat ctggcagcat tcaaggcagc 6180aacaaacaag ctgttccagt tagacttagc ggtcatttat gatgactggt atgatgccta 6240tacaagaaaa gattgcatac ggttacgtat tgaggacagg agtggaaacc tgattgatac 6300tagcaccttc taccaccacg acgaggacgt tctgttcaat atgtgtactg attggttgaa 6360ccatatgtat gaccagttga aggactggaa gtaatacgac tcagtatagg gacaatgctt 6420aaggtcgctc tctaggagtg gccttagtca tttaaccaat aggagataaa cattatgatg 6480aacattaaga ctaacccgtt taaagccgtg tctttcgtag agtctgccat taagaaggct 6540ctggataacg ctgggtatct tatcgctgaa atcaagtacg atggtgtacg cgggaacatc 6600tgcgtagaca atactgctaa cagttactgg ctctctcgtg tatctaaaac gattccggca 6660ctggagcact taaacgggtt tgatgttcgc tggaagcgtc tactgaacga tgaccgttgc 6720ttctacaaag atggctttat gcttgatggg gaactcatgg tcaagggcgt agactttaac 6780acagggtccg gcctactgcg taccaaatgg actgacacga agaaccaaga gttccatgaa 6840gagttattcg ttgaaccaat ccgtaagaaa gataaagttc cctttaagct gcacactgga 6900caccttcaca taaaactgta cgctatcctc ccgctgcaca tcgtggagtc tggagaagac 6960tgtgatgtca tgacgttgct catgcaggaa cacgttaaga acatgctgcc tctgctacag 7020gaatacttcc ctgaaatcga atggcaagcg gctgaatctt acgaggtcta cgatatggta 7080gaactacagc aactgtacga gcagaagcga gcagaaggcc atgagggtct cattgtgaaa 7140gacccgatgt gtatctataa gcgcggtaag aaatctggct ggtggaaaat gaaacctgag 7200aacgaagctg acggtatcat tcagggtctg gtatggggta caaaaggtct ggctaatgaa 7260ggtaaagtga ttggttttga ggtgcttctt gagagtggtc gtttagttaa cgccacgaat 7320atctctcgcg ccttaatgga tgagttcact gagacagtaa aagaggccac cctaagtcaa 7380tggggattct ttagcccata cggtattggc gacaacgatg cttgtactat taacccttac 7440gatggctggg cgtgtcaaat tagctacatg gaggaaacac ctgatggctc tttgcggcac 7500ccatcgttcg taatgttccg tggcaccgag gacaaccctc aagagaaaat gtaatcacac 7560tggctcacct tcgggtgggc ctttctgcgt ttataaggag acactttatg tttaagaagg 7620ttggtaaatt ccttgcggct ttggcagcta tcctgacgct tgcgtatatt cttgcggtat 7680accctcaagt agcactagta gtagttggcg cttgttactt agcggcagtg tgtgcttgcg 7740tgtggagtat agttaactgg taatacgact cactaaagga ggtacacacc atgatgtact 7800taatgccatt actcatcgtc attgtaggat gccttgcgct ccactgtagc gatgatgata 7860tgccagatgg tcacgcttaa tacgactcac taaaggagac actatatgtt tcgacttcat 7920tacaacaaaa gcgttaagaa tttcacggtt cgccgtgctg accgttcaat cgtatgtgcg 7980agcgagcgcc gagctaagat acctcttatt ggtaacacag ttcctttggc accgagcgtc 8040cacatcatta tcacccgtgg tgactttgag aaagcaatag acaagaaacg tccggttctt 8100agtgtggcag tgacccgctt cccgttcgtc cgtctgttac tcaaacgaat caaggaggtg 8160ttctgatggg actgttagat ggtgaagcct gggaaaaaga aaacccgcca gtacaagcaa 8220ctgggtgtat agcttgctta gagaaagatg accgttatcc acacacctgt aacaaaggag 8280ctaacgatat gaccgaacgt gaacaagaga tgatcattaa gttgatagac aataatgaag 8340gtcgcccaga tgatttgaat ggctgcggta ttctctgctc caatgtccct tgccacctct 8400gccccgcaaa taacgatcaa aagataacct taggtgaaat ccgagcgatg gacccacgta 8460aaccacatct gaataaacct gaggtaactc ctacagatga ccagccttcc gctgagacaa 8520tcgaaggtgt cactaagcct tcccactaca tgctgtttga cgacattgag gctatcgaag 8580tgattgctcg ttcaatgacc gttgagcagt tcaagggata ctgcttcggt aacatcttaa 8640agtacagact acgtgctggt aagaagtcag agttagcgta cttagagaaa gacctagcga 8700aagcagactt ctataaagaa ctctttgaga aacataagga taaatgttat gcataacttc 8760aagtcaaccc cacctgccga cagcctatct gatgacttca catcttgctc agagtggtgc 8820cgaaagatgt gggaagagac attcgacgat gcgtacatca agctgtatga actttggaaa 8880tcgagaggtc aatgactatg tcaaacgtaa atacaggttc acttagtgtg gacaataaga 8940agttttgggc taccgtagag tcctcggagc attccttcga ggttccaatc tacgctgaga 9000ccctagacga agctctggag ttagccgaat ggcaatacgt tccggctggc tttgaggtta 9060ctcgtgtgcg tccttgtgta gcaccgaagt aatacgactc actattaggg aagactccct 9120ctgagaaacc aaacgaaacc taaaggagat taacattatg gctaagaaga ttttcacctc 9180tgcgctgggt accgctgaac cttacgctta catcgccaag ccggactacg gcaacgaaga 9240gcgtggcttt gggaaccctc gtggtgtcta taaagttgac ctgactattc ccaacaaaga 9300cccgcgctgc cagcgtatgg tcgatgaaat cgtgaagtgt cacgaagagg cttatgctgc 9360tgccgttgag gaatacgaag ctaatccacc tgctgtagct cgtggtaaga aaccgctgaa 9420accgtatgag ggtgacatgc cgttcttcga taacggtgac ggtacgacta cctttaagtt 9480caaatgctac gcgtctttcc aagacaagaa gaccaaagag accaagcaca tcaatctggt 9540tgtggttgac tcaaaaggta agaagatgga agacgttccg attatcggtg gtggctctaa 9600gctgaaagtt aaatattctc tggttccata caagtggaac actgctgtag gtgcgagcgt 9660taagctgcaa ctggaatccg tgatgctggt cgaactggct acctttggtg gcggtgaaga 9720cgattgggct gacgaagttg aagagaacgg ctatgttgcc tctggttctg ccaaagcgag 9780caaaccacgc gacgaagaaa gctgggacga agacgacgaa gagtccgagg aagcagacga 9840agacggagac ttctaagtgg aactgcggga gaaaatcctt gagcgaatca aggtgacttc 9900ctctgggtgt tgggagtggc agggcgctac gaacaataaa gggtacgggc aggtgtggtg 9960cagcaatacc ggaaaggttg tctactgtca tcgcgtaatg tctaatgctc cgaaaggttc 10020taccgtcctg cactcctgtg ataatccatt atgttgtaac cctgaacacc tatccatagg 10080aactccaaaa gagaactcca ctgacatggt aaataagggt cgctcacaca aggggtataa 10140actttcagac gaagacgtaa tggcaatcat ggagtccagc gagtccaatg tatccttagc 10200tcgcacctat ggtgtctccc aacagactat ttgtgatata cgcaaaggga ggcgacatgg 10260caggttacgg cgctaaagga atccgaaagg ttggagcgtt tcgctctggc ctagaggaca 10320aggtttcaaa gcagttggaa tcaaaaggta ttaaattcga gtatgaagag tggaaagtgc 10380cttatgtaat tccggcgagc aatcacactt acactccaga cttcttactt ccaaacggta 10440tattcgttga gacaaagggt ctgtgggaaa gcgatgatag aaagaagcac ttattaatta 10500gggagcagca ccccgagcta gacatccgta ttgtcttctc aagctcacgt actaagttat 10560acaaaggttc tccaacgtct tatggagagt tctgcgaaaa gcatggtatt aagttcgctg 10620ataaactgat acctgctgag tggataaagg aacccaagaa ggaggtcccc tttgatagat 10680taaaaaggaa aggaggaaag aaataatggc tcgtgtacag tttaaacaac gtgaatctac 10740tgacgcaatc tttgttcact gctcggctac caagccaagt cagaatgttg gtgtccgtga 10800gattcgccag tggcacaaag agcagggttg gctcgatgtg ggataccact ttatcatcaa 10860gcgagacggt actgtggagg caggacgaga tgagatggct gtaggctctc acgctaaggg 10920ttacaaccac aactctatcg gcgtctgcct tgttggtggt atcgacgata aaggtaagtt 10980cgacgctaac tttacgccag cccaaatgca atcccttcgc tcactgcttg tcacactgct 11040ggctaagtac gaaggcgctg tgcttcgcgc ccatcatgag gtggcgccga aggcttgccc 11100ttcgttcgac cttaagcgtt ggtgggagaa gaacgaactg gtcacttctg accgtggata 11160attaattgaa ctcactaaag ggagaccaca gcggtttccc tttgttcgca ttggaggtca 11220aataatgcgc aagtcttata aacaattcta taaggctccg aggaggcata tccaagtgtg 11280ggaggcagcc aatgggccta taccaaaagg ttattatata gaccacattg acggcaatcc 11340actcaacgac gccttagaca atctccgtct ggctctccca aaagaaaact catggaacat 11400gaagactcca aagagcaata cctcaggact aaagggactg agttggagca aggaaaggga 11460gatgtggaga ggcactgtaa cagctgaggg taaacagcat aactttcgta gtagagatct 11520attggaagtc gttgcgtgga tttatagaac taggagggaa ttgcatggac aattcgcacg 11580attccgatag tgtatttctt taccacattc cttgtgacaa ctgtgggagt agtgatggga 11640actcgctgtt ctctgacgga cacacgttct gctacgtatg cgagaagtgg actgctggta 11700atgaagacac taaagagagg gcttcaaaac ggaaaccctc aggaggtaaa ccaatgactt 11760acaacgtgtg gaacttcggg gaatccaatg gacgctactc cgcgttaact gcgagaggaa 11820tctccaagga aacctgtcag aaggctggct actggattgc caaagtagac ggtgtgatgt 11880accaagtggc tgactatcgg gaccagaacg gcaacattgt gagtcagaag gttcgagata 11940aagataagaa ctttaagacc actggtagtc acaagagtga cgctctgttc gggaagcact 12000tgtggaatgg tggtaagaag attgtcgtta cagaaggtga aatcgacatg cttaccgtga 12060tggaacttca agactgtaag tatcctgtag tgtcgttggg tcacggtgcc tctgccgcta 12120agaagacatg cgctgccaac tacgaatact ttgaccagtt cgaacagatt atcttaatgt 12180tcgatatgga cgaagcaggg cgcaaagcag tcgaagaggc tgcacaggtt ctacctgctg 12240gtaaggtacg agtggcagtt cttccgtgta aggatgcaaa cgagtgtcac ctaaatggtc 12300acgaccgtga aatcatggag caagtgtgga atgctggtcc ttggattcct gatggtgtgg 12360tatcggctct ttcgttacgt gaacgaatcc gtgagcacct atcgtccgag gaatcagtag 12420gtttactttt cagtggctgc actggtatca acgataagac cttaggtgcc cgtggtggtg 12480aagtcattat ggtcacttcc ggttccggta tgggtaagtc aacgttcgtc cgtcaacaag 12540ctctacaatg gggcacagcg atgggcaaga aggtaggctt agcgatgctt gaggagtccg 12600ttgaggagac cgctgaggac cttataggtc tacacaaccg tgtccgactg agacaatccg 12660actcactaaa gagagagatt attgagaacg gtaagttcga ccaatggttc gatgaactgt 12720tcggcaacga tacgttccat ctatatgact cattcgccga ggctgagacg gatagactgc 12780tcgctaagct ggcctacatg cgctcaggct tgggctgtga cgtaatcatt ctagaccaca 12840tctcaatcgt cgtatccgct tctggtgaat ccgatgagcg taagatgatt gacaacctga 12900tgaccaagct caaagggttc gctaagtcaa ctggggtggt gctggtcgta atttgtcacc 12960ttaagaaccc agacaaaggt aaagcacatg aggaaggtcg ccccgtttct attactgacc 13020tacgtggttc tggcgcacta cgccaactat ctgatactat tattgccctt gagcgtaatc 13080agcaaggcga tatgcctaac cttgtcctcg ttcgtattct caagtgccgc tttactggtg 13140atactggtat cgctggctac atggaataca acaaggaaac cggatggctt gaaccatcaa 13200gttactcagg ggaagaagag tcacactcag agtcaacaga ctggtccaac gacactgact 13260tctgacagga ttcttgatga ctttccagac gactacgaga agtttcgctg gagagtccca 13320ttctaatacg actcactaaa ggagacacac catgttcaaa ctgattaaga agttaggcca 13380actgctggtt cgtatgtaca acgtggaagc caagcgactg aacgatgagg ctcgtaaaga 13440ggccacacag tcacgcgctc tggcgattcg ctccaacgaa ctggctgaca gtgcatccac 13500taaagttacc gaggctgccc gtgtggcaaa ccaagctcaa cagctttcca aattctttga 13560gtaatcaaac aggagaaacc attatgtcta acgtagctga aactatccgt ctatccgata 13620cagctgacca gtggaaccgt cgagtccaca tcaacgttcg caacggtaag gcgactatgg 13680tttaccgctg gaaggactct aagtcctcta agaatcacac tcagcgtatg acgttgacag 13740atgagcaagc actgcgtctg gtcaatgcgc ttaccaaagc tgccgtgaca gcaattcatg 13800aagctggtcg cgtcaatgaa gctatggcta tcctcgacaa gattgataac taagagtggt 13860atcctcaagg tcgccaaagt ggtggccttc atgaatacta ttcgactcac tataggagat 13920attaccatgc gtgaccctaa agttatccaa gcagaaatcg ctaaactgga agctgaactg 13980gaggacgtta agtaccatga agctaagact cgctccgctg ttcacatctt gaagaactta 14040ggctggactt ggacaagaca gactggctgg aagaaaccag aagttaccaa gctgagtcat 14100aaggtgttcg ataaggacac tatgacccac atcaaggctg gtgattgggt taaggttgac 14160atgggagttg ttggtggata cggctacgtc cgctcagtta gtggcaaata tgcacaagtg 14220tcatacatca caggtgttac tccacgcggt gcaatcgttg ccgataagac caacatgatt 14280cacacaggtt tcttgacagt tgtttcatat gaagagattg ttaagtcacg ataatcaata 14340ggagaaatca atatgatcgt ttctgacatc gaagctaacg ccctcttaga gagcgtcact 14400aagttccact gcggggttat ctacgactac tccaccgctg agtacgtaag ctaccgtccg 14460agtgacttcg gtgcgtatct ggatgcgctg gaagccgagg ttgcacgagg cggtcttatt 14520gtgttccaca acggtcacaa gtatgacgtt cctgcattga ccaaactggc aaagttgcaa 14580ttgaaccgag agttccacct tcctcgtgag aactgtattg acacccttgt gttgtcacgt 14640ttgattcatt ccaacctcaa ggacaccgat atgggtcttc tgcgttccgg caagttgccc 14700ggaaaacgct ttgggtctca cgctttggag gcgtggggtt atcgcttagg cgagatgaag 14760ggtgaataca aagacgactt taagcgtatg cttgaagagc agggtgaaga atacgttgac 14820ggaatggagt ggtggaactt caacgaagag atgatggact ataacgttca ggacgttgtg 14880gtaactaaag ctctccttga gaagctactc tctgacaaac attacttccc tcctgagatt 14940gactttacgg acgtaggata cactacgttc tggtcagaat cccttgaggc cgttgacatt 15000gaacatcgtg

ctgcatggct gctcgctaaa caagagcgca acgggttccc gtttgacaca 15060aaagcaatcg aagagttgta cgtagagtta gctgctcgcc gctctgagtt gctccgtaaa 15120ttgaccgaaa cgttcggctc gtggtatcag cctaaaggtg gcactgagat gttctgccat 15180ccgcgaacag gtaagccact acctaaatac cctcgcatta agacacctaa agttggtggt 15240atctttaaga agcctaagaa caaggcacag cgagaaggcc gtgagccttg cgaacttgat 15300acccgcgagt acgttgctgg tgctccttac accccagttg aacatgttgt gtttaaccct 15360tcgtctcgtg accacattca gaagaaactc caagaggctg ggtgggtccc gaccaagtac 15420accgataagg gtgctcctgt ggtggacgat gaggtactcg aaggagtacg tgtagatgac 15480cctgagaagc aagccgctat cgacctcatt aaagagtact tgatgattca gaagcgaatc 15540ggacagtctg ctgagggaga caaagcatgg cttcgttatg ttgctgagga tggtaagatt 15600catggttctg ttaaccctaa tggagcagtt acgggtcgtg cgacccatgc gttcccaaac 15660cttgcgcaaa ttccgggtgt acgttctcct tatggagagc agtgtcgcgc tgcttttggc 15720gctgagcacc atttggatgg gataactggt aagccttggg ttcaggctgg catcgacgca 15780tccggtcttg agctacgctg cttggctcac ttcatggctc gctttgataa cggcgagtac 15840gctcacgaga ttcttaacgg cgacatccac actaagaacc agatagctgc tgaactacct 15900acccgagata acgctaagac gttcatctat gggttcctct atggtgctgg tgatgagaag 15960attggacaga ttgttggtgc tggtaaagag cgcggtaagg aactcaagaa gaaattcctt 16020gagaacaccc ccgcgattgc agcactccgc gagtctatcc aacagacact tgtcgagtcc 16080tctcaatggg tagctggtga gcaacaagtc aagtggaaac gccgctggat taaaggtctg 16140gatggtcgta aggtacacgt tcgtagtcct cacgctgcct tgaataccct actgcaatct 16200gctggtgctc tcatctgcaa actgtggatt atcaagaccg aagagatgct cgtagagaaa 16260ggcttgaagc atggctggga tggggacttt gcgtacatgg catgggtaca tgatgaaatc 16320caagtaggct gccgtaccga agagattgct caggtggtca ttgagaccgc acaagaagcg 16380atgcgctggg ttggagacca ctggaacttc cggtgtcttc tggataccga aggtaagatg 16440ggtcctaatt gggcgatttg ccactgatac aggaggctac tcatgaacga aagacactta 16500acaggtgctg cttctgaaat gctagtagcc tacaaattta ccaaagctgg gtacactgtc 16560tattacccta tgctgactca gagtaaagag gacttggttg tatgtaagga tggtaaattt 16620agtaaggttc aggttaaaac agccacaacg gttcaaacca acacaggaga tgccaagcag 16680gttaggctag gtggatgcgg taggtccgaa tataaggatg gagactttga cattcttgcg 16740gttgtggttg acgaagatgt gcttattttc acatgggacg aagtaaaagg taagacatcc 16800atgtgtgtcg gcaagagaaa caaaggcata aaactatagg agaaattatt atggctatga 16860caaagaaatt taaagtgtcc ttcgacgtta ccgcaaagat gtcgtctgac gttcaggcaa 16920tcttagagaa agatatgctg catctatgta agcaggtcgg ctcaggtgcg attgtcccca 16980atggtaaaca gaaggaaatg attgtccagt tcctgacaca cggtatggaa ggattgatga 17040cattcgtagt acgtacatca tttcgtgagg ccattaagga catgcacgaa gagtatgcag 17100ataaggactc tttcaaacaa tctcctgcaa cagtacggga ggtgttctga tgtctgacta 17160cctgaaagtg ctgcaagcaa tcaaaagttg ccctaagact ttccagtcca actatgtacg 17220gaacaatgcg agcctcgtag cggaggccgc ttcccgtggt cacatctcgt gcctgactac 17280tagtggacgt aacggtggcg cttgggaaat cactgcttcc ggtactcgct ttctgaaacg 17340aatgggagga tgtgtctaat gtctcgtgac cttgtgacta ttccacgcga tgtgtggaac 17400gatatacagg gctacatcga ctctctggaa cgtgagaacg atagccttaa gaatcaacta 17460atggaagctg acgaatacgt agcggaacta gaggagaaac ttaatggcac ttcttgacct 17520taaacaattc tatgagttac gtgaaggctg cgacgacaag ggtatccttg tgatggacgg 17580cgactggctg gtcttccaag ctatgagtgc tgctgagttt gatgcctctt gggaggaaga 17640gatttggcac cgatgctgtg accacgctaa ggcccgtcag attcttgagg attccattaa 17700gtcctacgag acccgtaaga aggcttgggc aggtgctcca attgtccttg cgttcaccga 17760tagtgttaac tggcgtaaag aactggttga cccgaactat aaggctaacc gtaaggccgt 17820gaagaaacct gtagggtact ttgagttcct tgatgctctc tttgagcgcg aagagttcta 17880ttgcatccgt gagcctatgc ttgagggtga tgacgttatg ggagttattg cttccaatcc 17940gtctgccttc ggtgctcgta aggctgtaat catctcttgc gataaggact ttaagaccat 18000ccctaactgt gacttcctgt ggtgtaccac tggtaacatc ctgactcaga ccgaagagtc 18060cgctgactgg tggcacctct tccagaccat caagggtgac atcactgatg gttactcagg 18120gattgctgga tggggtgata ccgccgagga cttcttgaat aacccgttca taaccgagcc 18180taaaacgtct gtgcttaagt ccggtaagaa caaaggccaa gaggttacta aatgggttaa 18240acgcgaccct gagcctcatg agacgctttg ggactgcatt aagtccattg gcgcgaaggc 18300tggtatgacc gaagaggata ttatcaagca gggccaaatg gctcgaatcc tacggttcaa 18360cgagtacaac tttattgaca aggagattta cctgtggaga ccgtagcgta tattggtctg 18420ggtctttgtg ttctcggagt gtgcctcatt tcgtggggcc tttgggactt agccagaata 18480atcaagtcgt tacacgacac taagtgataa actcaaggtc cctaaattaa tacgactcac 18540tatagggaga taggggcctt tacgattatt actttaagat ttaactctaa gaggaatctt 18600tattatgtta acacctatta accaattact taagaaccct aacgatattc cagatgtacc 18660tcgtgcaacc gctgagtatc tacaggttcg attcaactat gcgtacctcg aagcgtctgg 18720tcatatagga cttatgcgtg ctaatggttg tagtgaggcc cacatcttgg gtttcattca 18780gggcctacag tatgcctcta acgtcattga cgagattgag ttacgcaagg aacaactaag 18840agatgatggg gaggattgac actatgtgtt tctcaccgaa aattaaaact ccgaagatgg 18900ataccaatca gattcgagcc gttgagccag cgcctctgac ccaagaagtg tcaagcgtgg 18960agttcggtgg gtcttctgat gagacggata ccgagggcac cgaagtgtct ggacgcaaag 19020gcctcaaggt cgaacgtgat gattccgtag cgaagtctaa agccagcggc aatggctccg 19080ctcgtatgaa atcttccatc cgtaagtccg catttggagg taagaagtga tgtctgagtt 19140cacatgtgtg gaggctaaga gtcgcttccg tgcaatccgg tggactgtgg aacaccttgg 19200gttgcctaaa ggattcgaag gacactttgt gggctacagc ctctacgtag acgaagtgat 19260ggacatgtct ggttgccgtg aagagtacat tctggactct accggaaaac atgtagcgta 19320cttcgcgtgg tgcgtaagct gtgacattca ccacaaagga gacattctgg atgtaacgtc 19380cgttgtcatt aatcctgagg cagactctaa gggcttacag cgattcctag cgaaacgctt 19440taagtacctt gcggaactcc acgattgcga ttgggtgtct cgttgtaagc atgaaggcga 19500gacaatgcgt gtatacttta aggaggtata agttatgggt aagaaagtta agaaggccgt 19560gaagaaagtc accaagtccg ttaagaaagt cgttaaggaa ggggctcgtc cggttaaaca 19620ggttgctggc ggtctagctg gtctggctgg tggtactggt gaagcacaga tggtggaagt 19680accacaagct gccgcacaga ttgttgacgt acctgagaaa gaggtttcca ctgaggacga 19740agcacagaca gaaagcggac gcaagaaagc tcgtgctggc ggtaagaaat ccttgagtgt 19800agcccgtagc tccggtggcg gtatcaacat ttaatcagga ggttatcgtg gaagactgca 19860ttgaatggac cggaggtgtc aactctaagg gttatggtcg taagtgggtt aatggtaaac 19920ttgtgactcc acataggcac atctatgagg agacatatgg tccagttcca acaggaattg 19980tggtgatgca tatctgcgat aaccctaggt gctataacat aaagcacctt acgcttggaa 20040ctccaaagga taattccgag gacatggtta ccaaaggtag acaggctaaa ggagaggaac 20100taagcaagaa acttacagag tcagacgttc tcgctatacg ctcttcaacc ttaagccacc 20160gctccttagg agaactgtat ggagtcagtc aatcaaccat aacgcgaata ctacagcgta 20220agacatggag acacatttaa tggctgagaa acgaacagga cttgcggagg atggcgcaaa 20280gtctgtctat gagcgtttaa agaacgaccg tgctccctat gagacacgcg ctcagaattg 20340cgctcaatat accatcccat cattgttccc taaggactcc gataacgcct ctacagatta 20400tcaaactccg tggcaagccg tgggcgctcg tggtctgaac aatctagcct ctaagctcat 20460gctggctcta ttccctatgc agacttggat gcgacttact atatctgaat atgaagcaaa 20520gcagttactg agcgaccccg atggactcgc taaggtcgat gagggcctct cgatggtaga 20580gcgtatcatc atgaactaca ttgagtctaa cagttaccgc gtgactctct ttgaggctct 20640caaacagtta gtcgtagctg gtaacgtcct gctgtaccta ccggaaccgg aagggtcaaa 20700ctataatccc atgaagctgt accgattgtc ttcttatgtg gtccaacgag acgcattcgg 20760caacgttctg caaatggtga ctcgtgacca gatagctttt ggtgctctcc ctgaggacat 20820ccgtaaggct gtagaaggtc aaggtggtga gaagaaagct gatgagacaa tcgacgtgta 20880cactcacatc tatctggatg aggactcagg tgaatacctc cgatacgaag aggtcgaggg 20940tatggaagtc caaggctccg atgggactta tcctaaagag gcttgcccat acatcccgat 21000tcggatggtc agactagatg gtgaatccta cggtcgttcg tacattgagg aatacttagg 21060tgacttacgg tcccttgaaa atctccaaga ggctatcgtc aagatgtcca tgattagctc 21120taaggttatc ggcttagtga atcctgctgg tatcacccag ccacgccgac tgaccaaagc 21180tcagactggt gacttcgtta ctggtcgtcc agaagacatc tcgttcctcc aactggagaa 21240gcaagcagac tttactgtag ctaaagccgt aagtgacgct atcgaggctc gcctttcgtt 21300tgcctttatg ttgaactctg cggttcagcg tacaggtgaa cgtgtgaccg ccgaagagat 21360tcggtatgta gcttctgaac ttgaagatac tttaggtggt gtctactcta tcctttctca 21420agaattacaa ttgcctctgg tacgagtgct cttgaagcaa ctacaagcca cgcaacagat 21480tcctgagtta cctaaggaag ccgtagagcc aaccattagt acaggtctgg aagcaattgg 21540tcgaggacaa gaccttgata agctggagcg gtgtgtcact gcgtgggctg cactggcacc 21600tatgcgggac gaccctgata ttaaccttgc gatgattaag ttacgtattg ccaacgctat 21660cggtattgac acttctggta ttctactcac cgaagaacag aagcaacaga agatggccca 21720acagtctatg caaatgggta tggataatgg tgctgctgcg ctggctcaag gtatggctgc 21780acaagctaca gcttcacctg aggctatggc tgctgccgct gattccgtag gtttacagcc 21840gggaatttaa tacgactcac tatagggaga cctcatcttt gaaatgagcg atgacaagag 21900gttggagtcc tcggtcttcc tgtagttcaa ctttaaggag acaataataa tggctgaatc 21960taatgcagac gtatatgcat cttttggcgt gaactccgct gtgatgtctg gtggttccgt 22020tgaggaacat gagcagaaca tgctggctct tgatgttgct gcccgtgatg gcgatgatgc 22080aatcgagtta gcgtcagacg aagtggaaac agaacgtgac ctgtatgaca actctgaccc 22140gttcggtcaa gaggatgacg aaggccgcat tcaggttcgt atcggtgatg gctctgagcc 22200gaccgatgtg gacactggag aagaaggcgt tgagggcacc gaaggttccg aagagtttac 22260cccactgggc gagactccag aagaactggt agctgcctct gagcaacttg gtgagcacga 22320agagggcttc caagagatga ttaacattgc tgctgagcgt ggcatgagtg tcgagaccat 22380tgaggctatc cagcgtgagt acgaggagaa cgaagagttg tccgccgagt cctacgctaa 22440gctggctgaa attggctaca cgaaggcttt cattgactcg tatatccgtg gtcaagaagc 22500tctggtggag cagtacgtaa acagtgtcat tgagtacgct ggtggtcgtg aacgttttga 22560tgcactgtat aaccaccttg agacgcacaa ccctgaggct gcacagtcgc tggataatgc 22620gttgaccaat cgtgacttag cgaccgttaa ggctatcatc aacttggctg gtgagtctcg 22680cgctaaggcg ttcggtcgta agccaactcg tagtgtgact aatcgtgcta ttccggctaa 22740acctcaggct accaagcgtg aaggctttgc ggaccgtagc gagatgatta aagctatgag 22800tgaccctcgg tatcgcacag atgccaacta tcgtcgtcaa gtcgaacaga aagtaatcga 22860ttcgaacttc tgatagactt cgaaattaat acgactcact atagggagac cacaacggtt 22920tccctctaga aataattttg tttaacttta agaaggagat atacatatgg ctagcatgac 22980tggtggacag caaatgggta ctaaccaagg taaaggtgta gttgctgctg gagataaact 23040ggcgttgttc ttgaaggtat ttggcggtga agtcctgact gcgttcgctc gtacctccgt 23100gaccacttct cgccacatgg tacgttccat ctccagcggt aaatccgctc agttccctgt 23160tctgggtcgc actcaggcag cgtatctggc tccgggcgag aacctcgacg ataaacgtaa 23220ggacatcaaa cacaccgaga aggtaatcac cattgacggt ctcctgacgg ctgacgttct 23280gatttatgat attgaggacg cgatgaacca ctacgacgtt cgctctgagt atacctctca 23340gttgggtgaa tctctggcga tggctgcgga tggtgcggtt ctggctgaga ttgccggtct 23400gtgtaacgtg gaaagcaaat ataatgagaa catcgagggc ttaggtactg ctaccgtaat 23460tgagaccact cagaacaagg ccgcacttac cgaccaagtt gcgctgggta aggagattat 23520tgcggctctg actaaggctc gtgcggctct gaccaagaac tatgttccgg ctgctgaccg 23580tgtgttctac tgtgacccag atagctactc tgcgattctg gcagcactga tgccgaacgc 23640agcaaactac gctgctctga ttgaccctga gaagggttct atccgcaacg ttatgggctt 23700tgaggttgta gaagttccgc acctcaccgc tggtggtgct ggtaccgctc gtgagggcac 23760tactggtcag aagcacgtct tccctgccaa taaaggtgag ggtaatgtca aggttgctaa 23820ggacaacgtt atcggcctgt tcatgcaccg ctctgcggta ggtactgtta agctgcgtga 23880cttggctctg gagcgcgctc gccgtgctaa cttccaagcg gaccagatta tcgctaagta 23940cgcaatgggc cacggtggtc ttcgcccaga agctgctggt gcagtggttt tcaaagtgga 24000gtaatgctgg gggtggcctc aacggtcgct gctagtcccg aagaggcgag tgttacttca 24060acagaagaaa ccttaacgcc agcacaggag gccgcacgca cccgcgctgc taacaaagcc 24120cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg 24180gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatg cgctcatacg 24240atatgaacgt tgagactgcc gctgagttat cagctgtgaa cgacattctg gcgtctatcg 24300gtgaacctcc ggtatcaacg ctggaaggtg acgctaacgc agatgcagcg aacgctcggc 24360gtattctcaa caagattaac cgacagattc aatctcgtgg atggacgttc aacattgagg 24420aaggcataac gctactacct gatgtttact ccaacctgat tgtatacagt gacgactatt 24480tatccctaat gtctacttcc ggtcaatcca tctacgttaa ccgaggtggc tatgtgtatg 24540accgaacgag tcaatcagac cgctttgact ctggtattac tgtgaacatt attcgtctcc 24600gcgactacga tgagatgcct gagtgcttcc gttactggat tgtcaccaag gcttcccgtc 24660agttcaacaa ccgattcttt ggggcaccgg aagtagaggg tgtactccaa gaagaggaag 24720atgaggctag acgtctctgc atggagtatg agatggacta cggtgggtac aatatgctgg 24780atggagatgc gttcacttct ggtctactga ctcgctaaca ttaataaata aggaggctct 24840aatggcactc attagccaat caatcaagaa cttgaagggt ggtatcagcc aacagcctga 24900catccttcgt tatccagacc aagggtcacg ccaagttaac ggttggtctt cggagaccga 24960gggcctccaa aagcgtccac ctcttgtttt cttaaataca cttggagaca acggtgcgtt 25020aggtcaagct ccgtacatcc acctgattaa ccgagatgag cacgaacagt attacgctgt 25080gttcactggt agcggaatcc gagtgttcga cctttctggt aacgagaagc aagttaggta 25140tcctaacggt tccaactaca tcaagaccgc taatccacgt aacgacctgc gaatggttac 25200tgtagcagac tatacgttca tcgttaaccg taacgttgtt gcacagaaga acacaaagtc 25260tgtcaactta ccgaattaca accctaatca agacggattg attaacgttc gtggtggtca 25320gtatggtagg gaactaattg tacacattaa cggtaaagac gttgcgaagt ataagatacc 25380agatggtagt caacctgaac acgtaaacaa tacggatgcc caatggttag ctgaagagtt 25440agccaagcag atgcgcacta acttgtctga ttggactgta aatgtagggc aagggttcat 25500ccatgtgacc gcacctagtg gtcaacagat tgactccttc acgactaaag atggctacgc 25560agaccagttg attaaccctg tgacccacta cgctcagtcg ttctctaagc tgccacctaa 25620tgctcctaac ggctacatgg tgaaaatcgt aggggacgcc tctaagtctg ccgaccagta 25680ttacgttcgg tatgacgctg agcggaaagt ttggactgag actttaggtt ggaacactga 25740ggaccaagtt ctatgggaaa ccatgccaca cgctcttgtg cgagccgctg acggtaattt 25800cgacttcaag tggcttgagt ggtctcctaa gtcttgtggt gacgttgaca ccaacccttg 25860gccttctttt gttggttcaa gtattaacga tgtgttcttc ttccgtaacc gcttaggatt 25920ccttagtggg gagaacatca tattgagtcg tacagccaaa tacttcaact tctaccctgc 25980gtccattgcg aaccttagtg atgacgaccc tatagacgta gctgtgagta ccaaccgaat 26040agcaatcctt aagtacgccg ttccgttctc agaagagtta ctcatctggt ccgatgaagc 26100acaattcgtc ctgactgcct cgggtactct cacatctaag tcggttgagt tgaacctaac 26160gacccagttt gacgtacagg accgagcgag accttttggg attgggcgta atgtctactt 26220tgctagtccg aggtccagct tcacgtccat ccacaggtac tacgctgtgc aggatgtcag 26280ttccgttaag aatgctgagg acattacatc acacgttcct aactacatcc ctaatggtgt 26340gttcagtatt tgcggaagtg gtacggaaaa cttctgttcg gtactatctc acggggaccc 26400tagtaaaatc ttcatgtaca aattcctgta cctgaacgaa gagttaaggc aacagtcgtg 26460gtctcattgg gactttgggg aaaacgtaca ggttctagct tgtcagagta tcagctcaga 26520tatgtatgtg attcttcgca atgagttcaa tacgttccta gctagaatct ctttcactaa 26580gaacgccatt gacttacagg gagaacccta tcgtgccttt atggacatga agattcgata 26640cacgattcct agtggaacat acaacgatga cacattcact acctctattc atattccaac 26700aatttatggt gcaaacttcg ggaggggcaa aatcactgta ttggagcctg atggtaagat 26760aaccgtgttt gagcaaccta cggctgggtg gaatagcgac ccttggctga gactcagcgg 26820taacttggag ggacgcatgg tgtacattgg gttcaacatt aacttcgtat atgagttctc 26880taagttcctc atcaagcaga ctgccgacga cgggtctacc tccacggaag acattgggcg 26940cttacagtta cgccgagcgt gggttaacta cgagaactct ggtacgtttg acatttatgt 27000tgagaaccaa tcgtctaact ggaagtacac aatggctggt gcccgattag gctctaacac 27060tctgagggct gggagactga acttagggac cggacaatat cgattccctg tggttggtaa 27120cgccaagttc aacactgtat acatcttgtc agatgagact acccctctga acatcattgg 27180gtgtggctgg gaaggtaact acttacggag aagttccggt atttaattaa atattctccc 27240tgtggtggct cgaaattaat acgactcact atagggagaa caatacgact acgggagggt 27300tttcttatga tgactataag acctactaaa agtacagact ttgaggtatt cactccggct 27360caccatgaca ttcttgaagc taaggctgct ggtattgagc cgagtttccc tgatgcttcc 27420gagtgtgtca cgttgagcct ctatgggttc cctctagcta tcggtggtaa ctgcggggac 27480cagtgctggt tcgttacgag cgaccaagtg tggcgactta gtggaaaggc taagcgaaag 27540ttccgtaagt taatcatgga gtatcgcgat aagatgcttg agaagtatga tactctttgg 27600aattacgtat gggtaggcaa tacgtcccac attcgtttcc tcaagactat cggtgcggta 27660ttccatgaag agtacacacg agatggtcaa tttcagttat ttacaatcac gaaaggagga 27720taaccatatg tgttgggcag ccgcaatacc tatcgctata tctggcgctc aggctatcag 27780tggtcagaac gctcaggcca aaatgattgc cgctcagacc gctgctggtc gtcgtcaagc 27840tatggaaatc atgaggcaga cgaacatcca gaatgctgac ctatcgttgc aagctcgaag 27900taaacttgag gaagcgtccg ccgagttgac ctcacagaac atgcagaagg tccaagctat 27960tgggtctatc cgagcggcta tcggagagag tatgcttgaa ggttcctcaa tggaccgcat 28020taagcgagtc acagaaggac agttcattcg ggaagccaat atggtaactg agaactatcg 28080ccgtgactac caagcaatct tcgcacagca acttggtggt actcaaagtg ctgcaagtca 28140gattgacgaa atctataaga gcgaacagaa acagaagagt aagctacaga tggttctgga 28200cccactggct atcatggggt cttccgctgc gagtgcttac gcatccggtg cgttcgactc 28260taagtccaca actaaggcac ctattgttgc cgctaaagga accaagacgg ggaggtaatg 28320agctatgagt aaaattgaat ctgcccttca agcggcacaa ccgggactct ctcggttacg 28380tggtggtgct ggaggtatgg gctatcgtgc agcaaccact caggccgaac agccaaggtc 28440aagcctattg gacaccattg gtcggttcgc taaggctggt gccgatatgt ataccgctaa 28500ggaacaacga gcacgagacc tagctgatga acgctctaac gagattatcc gtaagctgac 28560ccctgagcaa cgtcgagaag ctctcaacaa cgggaccctt ctgtatcagg atgacccata 28620cgctatggaa gcactccgag tcaagactgg tcgtaacgct gcgtatcttg tggacgatga 28680cgttatgcag aagataaaag agggtgtctt ccgtactcgc gaagagatgg aagagtatcg 28740ccatagtcgc cttcaagagg gcgctaaggt atacgctgag cagttcggca tcgaccctga 28800ggacgttgat tatcagcgtg gtttcaacgg ggacattacc gagcgtaaca tctcgctgta 28860tggtgcgcat gataacttct tgagccagca agctcagaag ggcgctatca tgaacagccg 28920agtggaactc aacggtgtcc ttcaagaccc tgatatgctg cgtcgtccag actctgctga 28980cttctttgag aagtatatcg acaacggtct ggttactggc gcaatcccat ctgatgctca 29040agccacacag cttataagcc aagcgttcag tgacgcttct agccgtgctg gtggtgctga 29100cttcctgatg cgagtcggtg acaagaaggt aacacttaac ggagccacta cgacttaccg 29160agagttgatt ggtgaggaac agtggaacgc tctcatggtc acagcacaac gttctcagtt 29220tgagactgac gcgaagctga acgagcagta tcgcttgaag attaactctg cgctgaacca 29280agaggaccca aggacagctt gggagatgct tcaaggtatc aaggctgaac tagataaggt 29340ccaacctgat gagcagatga caccacaacg tgagtggcta atctccgcac aggaacaagt 29400tcagaatcag atgaacgcat ggacgaaagc tcaggccaag gctctggacg attccatgaa 29460gtcaatgaac aaacttgacg taatcgacaa gcaattccag aagcgaatca acggtgagtg 29520ggtctcaacg gattttaagg atatgccagt caacgagaac actggtgagt tcaagcatag 29580cgatatggtt aactacgcca ataagaagct cgctgagatt gacagtatgg acattccaga 29640cggtgccaag gatgctatga agttgaagta ccttcaagcg gactctaagg acggagcatt 29700ccgtacagcc atcggaacca tggtcactga cgctggtcaa gagtggtctg ccgctgtgat 29760taacggtaag ttaccagaac gaaccccagc tatggatgct ctgcgcagaa tccgcaatgc 29820tgaccctcag ttgattgctg cgctataccc agaccaagct gagctattcc tgacgatgga 29880catgatggac aagcagggta ttgaccctca ggttattctt gatgccgacc gactgactgt 29940taagcggtcc aaagagcaac gctttgagga tgataaagca ttcgagtctg cactgaatgc 30000atctaaggct cctgagattg cccgtatgcc agcgtcactg cgcgaatctg cacgtaagat 30060ttatgactcc

gttaagtatc gctcggggaa cgaaagcatg gctatggagc agatgaccaa 30120gttccttaag gaatctacct acacgttcac tggtgatgat gttgacggtg ataccgttgg 30180tgtgattcct aagaatatga tgcaggttaa ctctgacccg aaatcatggg agcaaggtcg 30240ggatattctg gaggaagcac gtaagggaat cattgcgagc aacccttgga taaccaataa 30300gcaactgacc atgtattctc aaggtgactc catttacctt atggacacca caggtcaagt 30360cagagtccga tacgacaaag agttactctc gaaggtctgg agtgagaacc agaagaaact 30420cgaagagaaa gctcgtgaga aggctctggc tgatgtgaac aagcgagcac ctatagttgc 30480cgctacgaag gcccgtgaag ctgctgctaa acgagtccga gagaaacgta aacagactcc 30540taagttcatc tacggacgta aggagtaact aaaggctaca taaggaggcc ctaaatggat 30600aagtacgata agaacgtacc aagtgattat gatggtctgt tccaaaaggc tgctgatgcc 30660aacggggtct cttatgacct tttacgtaaa gtcgcttgga cagaatcacg atttgtgcct 30720acagcaaaat ctaagactgg accattaggc atgatgcaat ttaccaaggc aaccgctaag 30780gccctcggtc tgcgagttac cgatggtcca gacgacgacc gactgaaccc tgagttagct 30840attaatgctg ccgctaagca acttgcaggt ctggtaggga agtttgatgg cgatgaactc 30900aaagctgccc ttgcgtacaa ccaaggcgag ggacgcttgg gtaatccaca acttgaggcg 30960tactctaagg gagacttcgc atcaatctct gaggagggac gtaactacat gcgtaacctt 31020ctggatgttg ctaagtcacc tatggctgga cagttggaaa cttttggtgg cataacccca 31080aagggtaaag gcattccggc tgaggtagga ttggctggaa ttggtcacaa gcagaaagta 31140acacaggaac ttcctgagtc cacaagtttt gacgttaagg gtatcgaaca ggaggctacg 31200gcgaaaccat tcgccaagga cttttgggag acccacggag aaacacttga cgagtacaac 31260agtcgttcaa ccttcttcgg attcaaaaat gctgccgaag ctgaactctc caactcagtc 31320gctgggatgg ctttccgtgc tggtcgtctc gataatggtt ttgatgtgtt taaagacacc 31380attacgccga ctcgctggaa ctctcacatc tggactccag aggagttaga gaagattcga 31440acagaggtta agaaccctgc gtacatcaac gttgtaactg gtggttcccc tgagaacctc 31500gatgacctca ttaaattggc taacgagaac tttgagaatg actcccgcgc tgccgaggct 31560ggcctaggtg ccaaactgag tgctggtatt attggtgctg gtgtggaccc gcttagctat 31620gttcctatgg tcggtgtcac tggtaagggc tttaagttaa tcaataaggc tcttgtagtt 31680ggtgccgaaa gtgctgctct gaacgttgca tccgaaggtc tccgtacctc cgtagctggt 31740ggtgacgcag actatgcggg tgctgcctta ggtggctttg tgtttggcgc aggcatgtct 31800gcaatcagtg acgctgtagc tgctggactg aaacgcagta aaccagaagc tgagttcgac 31860aatgagttca tcggtcctat gatgcgattg gaagcccgtg agacagcacg aaacgccaac 31920tctgcggacc tctctcggat gaacactgag aacatgaagt ttgaaggtga acataatggt 31980gtcccttatg aggacttacc aacagagaga ggtgccgtgg tgttacatga tggctccgtt 32040ctaagtgcaa gcaacccaat caaccctaag actctaaaag agttctccga ggttgaccct 32100gagaaggctg cgcgaggaat caaactggct gggttcaccg agattggctt gaagaccttg 32160gggtctgacg atgctgacat ccgtagagtg gctatcgacc tcgttcgctc tcctactggt 32220atgcagtctg gtgcctcagg taagttcggt gcaacagctt ctgacatcca tgagagactt 32280catggtactg accagcgtac ttataatgac ttgtacaaag caatgtctga cgctatgaaa 32340gaccctgagt tctctactgg cggcgctaag atgtcccgtg aagaaactcg atacactatc 32400taccgtagag cggcactagc tattgagcgt ccagaactac agaaggcact cactccgtct 32460gagagaatcg ttatggacat cattaagcgt cactttgaca ccaagcgtga acttatggaa 32520aacccagcaa tattcggtaa cacaaaggct gtgagtatct tccctgagag tcgccacaaa 32580ggtacttacg ttcctcacgt atatgaccgt catgccaagg cgctgatgat tcaacgctac 32640ggtgccgaag gtttgcagga agggattgcc cgctcatgga tgaacagcta cgtctccaga 32700cctgaggtca aggccagagt cgatgagatg cttaaggaat tacacggggt gaaggaagta 32760acaccagaga tggtagagaa gtacgctatg gataaggctt atggtatctc ccactcagac 32820cagttcacca acagttccat aatagaagag aacattgagg gcttagtagg tatcgagaat 32880aactcattcc ttgaggcacg taacttgttt gattcggacc tatccatcac tatgccagac 32940ggacagcaat tctcagtgaa tgacctaagg gacttcgata tgttccgcat catgccagcg 33000tatgaccgcc gtgtcaatgg tgacatcgcc atcatggggt ctactggtaa aaccactaag 33060gaacttaagg atgagatttt ggctctcaaa gcgaaagctg agggagacgg taagaagact 33120ggcgaggtac atgctttaat ggataccgtt aagattctta ctggtcgtgc tagacgcaat 33180caggacactg tgtgggaaac ctcactgcgt gccatcaatg acctagggtt cttcgctaag 33240aacgcctaca tgggtgctca gaacattacg gagattgctg ggatgattgt cactggtaac 33300gttcgtgctc tagggcatgg tatcccaatt ctgcgtgata cactctacaa gtctaaacca 33360gtttcagcta aggaactcaa ggaactccat gcgtctctgt tcgggaagga ggtggaccag 33420ttgattcggc ctaaacgtgc tgacattgtg cagcgcctaa gggaagcaac tgataccgga 33480cctgccgtgg cgaacatcgt agggaccttg aagtattcaa cacaggaact ggctgctcgc 33540tctccgtgga ctaagctact gaacggaacc actaactacc ttctggatgc tgcgcgtcaa 33600ggtatgcttg gggatgttat tagtgccacc ctaacaggta agactacccg ctgggagaaa 33660gaaggcttcc ttcgtggtgc ctccgtaact cctgagcaga tggctggcat caagtctctc 33720atcaaggaac atatggtacg cggtgaggac gggaagttta ccgttaagga caagcaagcg 33780ttctctatgg acccacgggc tatggactta tggagactgg ctgacaaggt agctgatgag 33840gcaatgctgc gtccacataa ggtgtcctta caggattccc atgcgttcgg agcactaggt 33900aagatggtta tgcagtttaa gtctttcact atcaagtccc ttaactctaa gttcctgcga 33960accttctatg atggatacaa gaacaaccga gcgattgacg ctgcgctgag catcatcacc 34020tctatgggtc tcgctggtgg tttctatgct atggctgcac acgtcaaagc atacgctctg 34080cctaaggaga aacgtaagga gtacttggag cgtgcactgg acccaaccat gattgcccac 34140gctgcgttat ctcgtagttc tcaattgggt gctcctttgg ctatggttga cctagttggt 34200ggtgttttag ggttcgagtc ctccaagatg gctcgctcta cgattctacc taaggacacc 34260gtgaaggaac gtgacccaaa caaaccgtac acctctagag aggtaatggg cgctatgggt 34320tcaaaccttc tggaacagat gccttcggct ggctttgtgg ctaacgtagg ggctacctta 34380atgaatgctg ctggcgtggt caactcacct aataaagcaa ccgagcagga cttcatgact 34440ggtcttatga actccacaaa agagttagta ccgaacgacc cattgactca acagcttgtg 34500ttgaagattt atgaggcgaa cggtgttaac ttgagggagc gtaggaaata atacgactca 34560ctatagggag aggcgaaata atcttctccc tgtagtctct tagatttact ttaaggaggt 34620caaatggcta acgtaattaa aaccgttttg acttaccagt tagatggctc caatcgtgat 34680tttaatatcc cgtttgagta tctagcccgt aagttcgtag tggtaactct tattggtgta 34740gaccgaaagg tccttacgat taatacagac tatcgctttg ctacacgtac tactatctct 34800ctgacaaagg cttggggtcc agccgatggc tacacgacca tcgagttacg tcgagtaacc 34860tccactaccg accgattggt tgactttacg gatggttcaa tcctccgcgc gtatgacctt 34920aacgtcgctc agattcaaac gatgcacgta gcggaagagg cccgtgacct cactacggat 34980actatcggtg tcaataacga tggtcacttg gatgctcgtg gtcgtcgaat tgtgaaccta 35040gcgaacgccg tggatgaccg cgatgctgtt ccgtttggtc aactaaagac catgaaccag 35100aactcatggc aagcacgtaa tgaagcctta cagttccgta atgaggctga gactttcaga 35160aaccaagcgg agggctttaa gaacgagtcc agtaccaacg ctacgaacac aaagcagtgg 35220cgcgatgaga ccaagggttt ccgagacgaa gccaagcggt tcaagaatac ggctggtcaa 35280tacgctacat ctgctgggaa ctctgcttcc gctgcgcatc aatctgaggt aaacgctgag 35340aactctgcca cagcatccgc taactctgct catttggcag aacagcaagc agaccgtgcg 35400gaacgtgagg cagacaagct ggaaaattac aatggattgg ctggtgcaat tgataaggta 35460gatggaacca atgtgtactg gaaaggaaat attcacgcta acgggcgcct ttacatgacc 35520acaaacggtt ttgactgtgg ccagtatcaa cagttctttg gtggtgtcac taatcgttac 35580tctgtcatgg agtggggaga tgagaacgga tggctgatgt atgttcaacg tagagagtgg 35640acaacagcga taggcggtaa catccagtta gtagtaaacg gacagatcat cacccaaggt 35700ggagccatga ccggtcagct aaaattgcag aatgggcatg ttcttcaatt agagtccgca 35760tccgacaagg cgcactatat tctatctaaa gatggtaaca ggaataactg gtacattggt 35820agagggtcag ataacaacaa tgactgtacc ttccactcct atgtacatgg tacgacctta 35880acactcaagc aggactatgc agtagttaac aaacacttcc acgtaggtca ggccgttgtg 35940gccactgatg gtaatattca aggtactaag tggggaggta aatggctgga tgcttaccta 36000cgtgacagct tcgttgcgaa gtccaaggcg tggactcagg tgtggtctgg tagtgctggc 36060ggtggggtaa gtgtgactgt ttcacaggat ctccgcttcc gcaatatctg gattaagtgt 36120gccaacaact cttggaactt cttccgtact ggccccgatg gaatctactt catagcctct 36180gatggtggat ggttacgatt ccaaatacac tccaacggtc tcggattcaa gaatattgca 36240gacagtcgtt cagtacctaa tgcaatcatg gtggagaacg agtaattggt aaatcacaag 36300gaaagacgtg tagtccacgg atggactctc aaggaggtac aaggtgctat cattagactt 36360taacaacgaa ttgattaagg ctgctccaat tgttgggacg ggtgtagcag atgttagtgc 36420tcgactgttc tttgggttaa gccttaacga atggttctac gttgctgcta tcgcctacac 36480agtggttcag attggtgcca aggtagtcga taagatgatt gactggaaga aagccaataa 36540ggagtgatat gtatggaaaa ggataagagc cttattacat tcttagagat gttggacact 36600gcgatggctc agcgtatgct tgcggacctt tcggaccatg agcgtcgctc tccgcaactc 36660tataatgcta ttaacaaact gttagaccgc cacaagttcc agattggtaa gttgcagccg 36720gatgttcaca tcttaggtgg ccttgctggt gctcttgaag agtacaaaga gaaagtcggt 36780gataacggtc ttacggatga tgatatttac acattacagt gatatactca aggccactac 36840agatagtggt ctttatggat gtcattgtct atacgagatg ctcctacgtg aaatctgaaa 36900gttaacggga ggcattatgc tagaattttt acgtaagcta atcccttggg ttctcgctgg 36960gatgctattc gggttaggat ggcatctagg gtcagactca atggacgcta aatggaaaca 37020ggaggtacac aatgagtacg ttaagagagt tgaggctgcg aagagcactc aaagagcaat 37080cgatgcggta tctgctaagt atcaagaaga ccttgccgcg ctggaaggga gcactgatag 37140gattatttct gatttgcgta gcgacaataa gcggttgcgc gtcagagtca aaactaccgg 37200aacctccgat ggtcagtgtg gattcgagcc tgatggtcga gccgaacttg acgaccgaga 37260tgctaaacgt attctcgcag tgacccagaa gggtgacgca tggattcgtg cgttacagga 37320tactattcgt gaactgcaac gtaagtagga aatcaagtaa ggaggcaatg tgtctactca 37380atccaatcgt aatgcgctcg tagtggcgca actgaaagga gacttcgtgg cgttcctatt 37440cgtcttatgg aaggcgctaa acctaccggt gcccactaag tgtcagattg acatggctaa 37500ggtgctggcg aatggagaca acaagaagtt catcttacag gctttccgtg gtatcggtaa 37560gtcgttcatc acatgtgcgt tcgttgtgtg gtccttatgg agagaccctc agttgaagat 37620acttatcgta tcagcctcta aggagcgtgc agacgctaac tccatcttta ttaagaacat 37680cattgacctg ctgccattcc tatctgagtt aaagccaaga cccggacagc gtgactcggt 37740aatcagcttt gatgtaggcc cagccaatcc tgaccactct cctagtgtga aatcagtagg 37800tatcactggt cagttaactg gtagccgtgc tgacattatc attgcggatg acgttgagat 37860tccgtctaac agcgcaacta tgggtgcccg tgagaagcta tggactctgg ttcaggagtt 37920cgctgcgtta cttaaaccgc tgccttcctc tcgcgttatc taccttggta cacctcagac 37980agagatgact ctctataagg aacttgagga taaccgtggg tacacaacca ttatctggcc 38040tgctctgtac ccaaggacac gtgaagagaa cctctattac tcacagcgtc ttgctcctat 38100gttacgcgct gagtacgatg agaaccctga ggcacttgct gggactccaa cagacccagt 38160gcgctttgac cgtgatgacc tgcgcgagcg tgagttggaa tacggtaagg ctggctttac 38220gctacagttc atgcttaacc ctaaccttag tgatgccgag aagtacccgc tgaggcttcg 38280tgacgctatc gtagcggcct tagacttaga gaaggcccca atgcattacc agtggcttcc 38340gaaccgtcag aacatcattg aggaccttcc taacgttggc cttaagggtg atgacctgca 38400tacgtaccac gattgttcca acaactcagg tcagtaccaa cagaagattc tggtcattga 38460ccctagtggt cgcggtaagg acgaaacagg ttacgctgtg ctgtacacac tgaacggtta 38520catctacctt atggaagctg gaggtttccg tgatggctac tccgataaga cccttgagtt 38580actcgctaag aaggcaaagc aatggggagt ccagacggtt gtctacgaga gtaacttcgg 38640tgacggtatg ttcggtaagg tattcagtcc tatccttctt aaacaccaca actgtgcgat 38700ggaagagatt cgtgcccgtg gtatgaaaga gatgcgtatt tgcgataccc ttgagccagt 38760catgcagact caccgccttg taattcgtga tgaggtcatt agggccgact accagtccgc 38820tcgtgacgta gacggtaagc atgacgttaa gtactcgttg ttctaccaga tgacccgtat 38880cactcgtgag aaaggcgctc tggctcatga tgaccgattg gatgcccttg cgttaggcat 38940tgagtatctc cgtgagtcca tgcagttgga ttccgttaag gtcgagggtg aagtacttgc 39000tgacttcctt gaggaacaca tgatgcgtcc tacggttgct gctacgcata tcattgagat 39060gtctgtggga ggagttgatg tgtactctga ggacgatgag ggttacggta cgtctttcat 39120tgagtggtga tttatgcatt aggactgcat agggatgcac tatagaccac ggatggtcag 39180ttctttaagt tactgaaaag acacgataaa ttaatacgac tcactatagg gagaggaggg 39240acgaaaggtt actatataga tactgaatga atacttatag agtgcataaa gtatgcataa 39300tggtgtacct agagtgacct ctaagaatgg tgattatatt gtattagtat caccttaact 39360taaggaccaa cataaaggga ggagactcat gttccgctta ttgttgaacc tactgcggca 39420tagagtcacc taccgatttc ttgtggtact ttgtgctgcc cttgggtacg catctcttac 39480tggagacctc agttcactgg agtctgtcgt ttgctctata ctcacttgta gcgattaggg 39540tcttcctgac cgactgatgg ctcaccgagg gattcagcgg tatgattgca tcacaccact 39600tcatccctat agagtcaagt cctaaggtat acccataaag agcctctaat ggtctatcct 39660aaggtctata cctaaagata ggccatccta tcagtgtcac ctaaagaggg tcttagagag 39720ggcctatgga gttcctatag ggtcctttaa aatataccat aaaaatctga gtgactatct 39780cacagtgtac ggacctaaag ttcccccata gggggtacct aaagcccagc caatcaccta 39840aagtcaacct tcggttgacc ttgagggttc cctaagggtt ggggatgacc cttgggtttg 39900tctttgggtg ttaccttgag tgtctctctg tgtccct 39937243769DNAArtificial SequenceSynthetic Polynucleotide 2tctctcggcc tcggcctcgc cgggatgtcc ccatagggtg cctgtgggcg ctagggcggc 60ctgtggaggc ctgagagaag ctcttagtgt gggccaaagg gtaacctgag gcctgccgga 120gcgagcgata gggacgcgtg taggccgctt gacagcgtgt gtgggcgtgg gctatctgtt 180cgtttgctcc gcttacgcta cgcttcactc acggccttgt gtaccttagg gtcttcctta 240tcgtgtacct tgggacagtc ttagtaacta ccttagtcac ttccttagta gcttccttag 300tgagtagctt agtggctatc tattgctgtc ttagtgttac cttagtgatt gcatagctac 360gctataagat gcgaataggt cgcggtcggt agaccgctaa agaaagagaa gaacaataag 420atgcagtagg agggacacca gaatcctagc cagcctaacc tatcctagct ctgtatctat 480tgcttttcct tagtccaaca cgttagacaa cctatgatta tcttagtagc tgtgacatgt 540atcacataaa taatctatct tagtgaaact tagtgttgac acaggcagta gtcggtagta 600cattacagtc atcgggaggc aacccagccg aacgataggt agctttggct gccttgctct 660ttaacaatat ggctagtgtc ttgataggct aactaactga ggttactatc atgctcaaag 720agactcaaat caagcacgaa aacggaaagt attgggtgtt agaggttaag aaaggtatgt 780atcaggtgat gatatctggc ttaactcact caacttgtga tagtgcttac aacgatctta 840gcttagctat ttatcggtgc gattatctgg ctaaacgagc ataaggtaag gctggcgtag 900gctggcctat caaggcacta tccttgctct ttaacaatct gcttagtgta acctatgtaa 960gccgtggtat tacttattaa cttaatgagg tgatactatg tacgatgaac tgtatgaagc 1020ttactttaac tctctggatg aaggagaaga ggtactatcc tttgctgatt ttgtagaggc 1080taggggaggt gctgaatgat gaccttgaat cttagagaag ctagcgcggt ctttactatg 1140ttatgttgga tgatacgtaa caacgaaatg atgaccgatg acgagctagc gctttaccac 1200cgctttcgta atgagggctg ggaagataca gtgaacaatg tgcgcgacat actgaaggag 1260ataatccatg tttaagcaca cgatatacac gcaatgctgc aattcagtgg gcattatgcg 1320ttggtgggat gagtctagtg ttaagtgcta caatttgaat gatgatagca ctatgtatga 1380ggttactctc attaaaagat ataaccacga cacgctgtta tggattctat ctgaatggga 1440actaacctat gaagatgtga ttacagaaga aatttaaatt aaccattgac taccacggct 1500tacataggtt acattaagca ccaacaagaa gtaacgatct ttaacaatct ggattgaagc 1560cgattagata gaggttaaca cataggaggt ttacgagcct cctagatggt aacttactaa 1620ctaagaggaa atagaaatgg caatgtctaa catgacttac agcgacgttt acaaccacgc 1680ttacggattg ctgaaagaat acattcgcta cgatgatgta cgcaacgagg acgacctgag 1740cgataaaatc cacgaggccg ctggtaatgc tgttccgcac tggtacgctg acatctttag 1800cgtaatggct agtgacggta ttgacttgga gttcgacgac tctggtctga tgcctgacac 1860taaggacgta acgtacatcc ttcaagctcg catccatgaa caactcacga ttgaccttta 1920cggggacgct gaagacctgc ttaatgagta tttagaagag attgaagctg aagaagacga 1980agaagaggac gaataaatga acggcaaaca atataccttt caattttctg atggtattac 2040cttgaaatgt tctctaaggt tcgccatgat gcgagaggaa acattaggaa ctagttataa 2100actagttatg tgacactata agatgattaa cagggtattc ttgcgagagt acccgattaa 2160tctaatttga tgaggcgatt atgagtaaag taacaaacat tttagtctct attgtaatcc 2220tgttagttgt gctgtggtct gcaataggtt ctaacttcca gtggtttaac acctgctatg 2280aaggagattt acacactaag cacttacagt ttaatggtgt tacaatatat tccacctttg 2340aaaaccataa agataaccct tttcataagt aatagcctat agtgtcattc gtggcactat 2400gtgaaattac ttaataacat atggagaaca taccatgact actgaataca ccattgtaac 2460tcttcgtgaa gctgcaaccg ctgaaatcaa agcacattta gacaccatcg gcgcttccta 2520tatcaagatt ggtacttgct taaacgagct acgcgctgac tttgacggtc aaaaggagtt 2580tttagcttat gttgaggctg aattctcaat taagaaagca caatgctatc acctgatgaa 2640tgtagcgcgt gttttcggtg aagatgagcg ctttaaaggt gtggcgatgc gtgtaatgtt 2700ggcgcttatt ccggtagctg atgaagcctc cgtaatgggt aaggccgcag aactggcggc 2760taatggtgag ctggatacta aggccgtaaa taaactgctt ggaaagcctc aggccacgcc 2820taaatctgaa cctaagcaat cacatggcga cgaagagaaa acgcctgaga gcgccgcaca 2880gggagcgcct cagccattgc agtcagtacc tgaggaagat aaagcgcctt gggatgaaga 2940caccacgcaa actgtgaaag atgattcaca gaaagcacct gagacagccg cgccgcgcct 3000ggataacgct gagaccgcag acagtgcggc tatggctagc ctgttagacc agattagcaa 3060gctgacagaa caactaacat tagctaacaa ccgcatcgcg gagttaacaa gcgctcgtga 3120atccaagaaa gcaagcgctc caatgctccc acagtttaaa tcttcatgtt tctatgctcg 3180cttaggtctg agcgcggagg aagcaaccaa gaaaacagca gttaacaagg ctaagcgtga 3240acttgttaag ctagggtatg gtgaaggtca tgaagcgtgg gccttgatta gcgaagcagt 3300agaatcctta actaaataaa gttgacttat agagcgtcat taagtaagat ggcgctcaat 3360taagttttct agtaccgcat gaggatacaa gatgcaagat ttacacgcta tccagcttca 3420attagaagaa gagatgttta atggtggcat tcgtcgcttc gaagcagatc aacaacgcca 3480gattgcagca ggtagcgaga gcgacacagc atggaaccgc cgcctgttgt cagaacttat 3540tgcacctatg gctgaaggca ttcaggctta taaagaagag tacgaaggta agaaaggtcg 3600tgcacctcgc gcattggctt tcttacaatg tgtagaaaat gaagttgcag catacatcac 3660tatgaaagtt gttatggata tgctgaatac ggatgctacc cttcaggcta ttgcaatgag 3720tgtagcagaa cgcattgaag accaagtgcg cttttctaag ctagaaggtc acgccgctaa 3780atactttgag aaggttaaga agtcactcaa ggctagccgt actaagtcat atcgtcacgc 3840tcataacgta gctgtagttg ctgaaaaatc agttgcagaa aaggacgcgg actttgaccg 3900ttgggaggcg tggccaaaag aaactcaatt gcagattggt actaccttgc ttgaaatctt 3960agaaggtagc gttttctata atggtgaacc tgtatttatg cgtgctatgc gcacttatgg 4020cggaaagact atttactact tacaaacttc tgaaagtgta ggccagtgga ttagcgcatt 4080caaagagcac gtagcgcaat taagcccagc ttatgcccct tgcgtaatcc ctcctcgtcc 4140ttggagaact ccatttaatg gagggttcca tactgagaag gtagctagcc gtatccgtct 4200tgtaaaaggt aaccgtgagc atgtacgcaa gttgactcaa aagcaaatgc caaaggttta 4260taaggctatc aacgcattac aaaatacaca atggcaaatc aacaaggatg tattagcagt 4320tattgaagaa gtaatccgct tagaccttgg ttatggtgta ccttccttca agccactgat 4380tgacaaggag aacaagccag ctaacccggt acctgttgaa ttccaacacc tgcgcggtcg 4440tgaactgaaa gagatgctat cacctgagca gtggcaacaa ttcattaact ggaaaggcga 4500atgcgcgcgc ctatataccg cagaaactaa gcgcggttca aagtccgccg ccgttgttcg 4560catggtagga caggcccgta aatatagcgc ctttgaatcc atttacttcg tgtacgcaat 4620ggatagccgc agccgtgtct atgtgcaatc tagcacgctc tctccgcagt ctaacgactt 4680aggtaaggca ttactccgct ttaccgaggg acgccctgtg aatggcgtag aagcgcttaa 4740atggttctgc atcaatggtg ctaacctttg gggatgggac aagaaaactt ttgatgtgcg 4800cgtgtctaac gtattagatg aggaattcca agatatgtgt cgagacatcg ccgcagaccc 4860tctcacattc acccaatggg ctaaagctga tgcaccttat gaattcctcg cttggtgctt 4920tgagtatgct caataccttg atttggtgga tgaaggaagg gccgacgaat tccgcactca 4980cctaccagta catcaggacg ggtcttgttc aggcattcag cactatagtg ctatgcttcg 5040cgacgaagta ggggccaaag ctgttaacct gaaaccctcc gatgcaccgc aggatatcta 5100tggggcggtg

gcgcaagtgg ttatcaagaa gaatgcgcta tatatggatg cggacgatgc 5160aaccacgttt acttctggta gcgtcacgct gtccggtaca gaactgcgag caatggctag 5220cgcatgggat agtattggta ttacccgtag cttaaccaaa aagcccgtga tgaccttgcc 5280atatggttct actcgcttaa cttgccgtga atctgtgatt gattacatcg tagacttaga 5340ggaaaaagag gcgcagaagg cagtagcaga agggcggacg gcaaacaagg tacatccttt 5400tgaagacgat cgtcaagatt acttgactcc gggcgcagct tacaactaca tgacggcact 5460aatctggcct tctatttctg aagtagttaa ggcaccgata gtagctatga agatgatacg 5520ccagcttgca cgctttgcag cgaaacgtaa tgaaggcctg atgtacaccc tgcctactgg 5580cttcatctta gaacagaaga tcatggcaac cgagatgcta cgcgtgcgta cctgtctgat 5640gggtgatatc aagatgtccc ttcaggttga aacggatatc gtagatgaag ccgctatgat 5700gggagcagca gcacctaatt tcgtacacgg tcatgacgca agtcacctta tccttaccgt 5760atgtgaattg gtagacaagg gcgtaactag tatcgctgta atccacgact cttttggtac 5820tcatgcagac aacaccctca ctcttagagt ggcacttaaa gggcagatgg ttgcaatgta 5880tattgatggt aatgcgcttc agaaactact ggaggagcat gaagagcgct ggatggttga 5940tacaggtatc gaagtacctg agcaagggga gttcgacctt aacgaaatca tggattctga 6000atacgtattt gcctaataga acaataaata tacaggtcag ccttcgggct ggccttttct 6060tttaactatt acctgtaaca tttaattaac aagtccaacg tgttggacac gatgcggatt 6120taagggacac tataggacta cccgtcggag acggaaagta ataggtaata ataggaagta 6180gtaggtaagt aaggtaatta taggttactt aggttactcc ttcctattac ctccttctta 6240ataggaaggg cagacactag gttgtctaac gtgttggaca gaacttattt acgtgacact 6300attgaactaa tcaacattca attcattgga gaattaatca tgcgtaactt tgagaaactg 6360acccgtaagc ctgctaatcg ttttggcatg gaggaaggga agacaggcgc caagcgtaac 6420aagcctaccc gtgaccgtgt atctaagcgt gcagtgtggg agtactaagt tatggctatt 6480attaacaata ttccgtgccc tgcctgtcaa aagaatggac atgataaatc tggcaatcat 6540cttatgatat ttgatgatgg cgctggttac tgcaatcgtg gacacttcca tgatagtggc 6600aagccttact accataagcc ggaaggtggc atcgaaatca ccgagctacc catcactggc 6660aatatcaaat atacaccttc tcaattcaaa gaaatggaga aggaagggaa gataagtgac 6720cctaaacttc gtgctatcgc cttgggtggt atgcgtatga aagatcgttg ggaggtgatg 6780aatgcggaag aaagggcgga gcaagaatct gaatggcagc ttgacgttga gtggttcctt 6840gaacttaaaa ggaagaacct tgtatcacga cacattcgcg gagacatttg tgcgctttat 6900gacgtccgag taggtcatga tggagaaggg aaggttaata ggcactacta ccctcgcttc 6960gaaggtggca aacttgtggg agctaagtgc cggacgctac ctaaagattt caagtttgga 7020catctaggta aactgtttgg caaccaagac atgttcggta tgaataccat gtctaacgtg 7080ttggacaagg gacgaaggaa agacaccctg cttatcgtgg gaggtgaact ggatgcacta 7140gcagcacagc agatgcttct ggattctgcc aaaggtacga agtgggaagg tcagccttac 7200catgtgtggt ctatcaacaa gggtgaggct tgccttgaag agatagtaca gaaccgtgag 7260cacatctctc agttcaagaa gattatgtgg ggcttcgacg gtgatgaaat agggcagaag 7320cttaaccaac aagcggcccg cctgttcccc ggcaagtctt atatcattga gtaccctgcg 7380ggctgcaagg atgctaacaa ggcattgatg gctggcaaat ccaaggagtt cgtagatgca 7440tggttcaatg ccaagtcatc agatgaggtt ttcggtagcc agattaaatc catcgcctct 7500caaagggaca agctgaaggc tgcacgccct gaaccgggat tatcttggcc ttggcctagg 7560ctgaacaaga taacccttgg catccgtaag catcagctaa tcatcgtcgg cgctggttct 7620ggtgtaggta agactgagtt cctccgcgaa gtagtgaagc acctcattga agaacatgga 7680gagtcggtag gtattatctc cactgaagac cctatggtta aggtctcccg cgcattcatt 7740ggtaaatgga ttgataagcg tattgaacta cctccaacca atgacccaag agaagatgga 7800taccgtgagg tctttgatta taccgaagag gaagccaacg ctgccattga ctacgttgct 7860gacactggta agctttttgt agctgacctt gaaggtgact attctatgga gaaggtagag 7920cagacgtgcc ttgagtttga ggcaatgggt atttctaaca tcatcattga taacttaaca 7980ggaattaaat tagatgaacg aaattttggt ggtaaagttg gtgcgcttga tgagtgcgtc 8040aaaaggattg gcactatcaa agaccgacat ccggttacta tcttccttgt ctcgcacctt 8100acacgtcctt caggacaacg tacctcacac gaagaaggtg gcgaggttat cctttctgac 8160ttccgaggct caggggctat cggattctgg gcttcttacg ccttggggat tgagcgtaat 8220acaagagctg aaacgcttga tgaaaggact accacgtaca tctcatgtgt caaagatcga 8280gaccaaggca tctacactgg tactaaagtg atgctcaaag gggatgttag taccggcaga 8340ttaatggaac cacaatcacg tactaaatca tttgatacag gtgctccaaa agagcaagct 8400gtgcctgatg aattaggtga cactatagaa gagaacacac aggagtttaa tggatgattt 8460aggttttggt tgttcgctac cgtactactt gttattaaca tagacaaggt tgctatgtta 8520ttcaaatagt gtacttatca gggtttgtct aacatgttgg acaaactctt attaagtaca 8580ttaactaact ggagattatt atgtgtaaat tgcacctcaa caaatcagat tgtgtgcgta 8640acattaacaa gagatctatc cgctttcgct gggagggtgt agtgtttgat gtagatgaga 8700gatactacca tgtagtgtat ggtaatggat tacgtcaaac ttatctgaag gctctggcgc 8760atcattacct tgaaccgatt gaaccaacta agagtaactg cacctgtgta cacgatgatc 8820tgtgtgatcg ctgtgctcgt caagttaata aggcattgac aatcatggag cgttacggtg 8880caggccacaa ggcaatctct gaggctgcgt ggactgtact catgtttgaa cgccctaatg 8940gtcgtaaggt gctgaatcgt gagcggcgta atgtaatcac aggtcaagac tttcgcatct 9000tagaggaggc tatgtgtaat cctggtattg ctatacgtta tgaggatgta gaccatgcta 9060tatctgaagg tatcggtaat cgtttggaat tgaataagca ttttgatcag gtattacgtg 9120acactatagg tgggcgcaaa ggttttacct ttgagcgcgg gcatgttaca tttaacccta 9180tcgttacgga ggaaacctat gtcacgcaat gacagtaagt acagcctgaa gttccttgag 9240cagcatgaag aacttgcagc caaggtaact aaccaagcat tcctgtttgc acaactaacg 9300ctggctgaag ctaagaagaa cagccttacg cgtgagcaga ttatcaagga aggaaccaag 9360cgcagttaat aagtcgtgac ttgtctaaca tgttggacag gtcactctca tattaattgg 9420agatacataa atgactaaag taactaagtt aaccgaacac ctgattaaac taagtgaaga 9480actaaagaac agcgaagtta ggcttgagta ttacttcatt gacccaaggg aagatgatcg 9540tgaaacacct gactacaagt ttgaaacgga gttaatgtat gaaaactatt aattgggcga 9600aggaagcaga aggacgtatc ctagtaatgg atgcggaggc taaaggctta cttgatgcaa 9660tccgatatgg aaaaggtaac gatgacgtgc atataatttg ctgcatggac ttgctcacca 9720ctgaagagtt tctcttcttc aacccatatg accgtcgtga ccctaacgca agggagcacc 9780tgaaggagtg ggatggtcat caggacggtg accttgaaga tggtgtgaga ttcctcaagc 9840actgtgaagc tatcgtgtca cagaacttcc tcggctatga cggcttgctt tttgagaagg 9900cattccccga tatatggaaa ggctataact acacggagaa gcgcggcaaa ggccgtctgc 9960gggccgatct gtgcccggtt aaggtaatgg atacccttgt catgtcaagg ctcctgaacc 10020cggataggcg actccctccg caggcatacg ctaagggtat gggtaacgtt gcacctcact 10080ctattgaggc acacggtatc cgtataggtc gctataagcc tgagaacgag gactggtcta 10140agctgacaga ccacatggtg caccgagtac gtgaggatgt ggcgatcggt cgtgacctgt 10200tcctgtggct gtacaacggc gagtggatgg agcacaagcg gcgtggcgtc aatccaagga 10260ctggtcttgg cattgagaca gccttccaca tggagtccat tgtagcactg gagatgtctc 10320gtcaagcgga gcgcggcttc cggctggata tagacaaggc actggcacga tgcgaggagc 10380ttgaccagaa gattgacgag actgttgcag ccttccggcc tcacatgcca atgcgcatca 10440agtctaagcc tttcaaacct caagagaaac aggagcaagt agatgcggca aactcattta 10500gtttacagaa tcatactggc gttacacttg gagccgatgc tttcattcat gccgagcggc 10560gctccgatag aaagactgta tggtcagtca ctactaagtc aggtgattgg tcagctactg 10620tcaagaaaga cttccctcac atccgaggaa acatcaatga tactccgagt attaaacaca 10680tcgggccata tacacctgtc accttcgaag atatcccgct tggtaaccga gacacagtta 10740agcaggttct gtatgacttt gggtggaggg gagttgagtt caacgacact gagcaatctt 10800atctggacga gcatggagtg ttgcctaagc cgtggagtgg aaagataaat gagaagtccc 10860ttactttatg gcaggaaagg gctgcacgtg aaggtaagtc agtacctgat tggtgcttgg 10920gtatcgctgc atggtacata ctcgtatccc gtcgtggtca gatcctcaac cgtggtgatg 10980ttgaaacctt cgattcaacg gggcgttggc cctcgcaagc tggtgtacga aagtgtcgcg 11040gcctcgtacc tgtagccttt aacaaggagc taggtatcaa tgcacaggca tactacgaaa 11100catatggcta ctggcctacg tccgacaagg atgatggaga gtggcgtgtt cccgctgttg 11160ctatttctat tggcacttct acgttccgta tgcgtcacag gaatgtggtt aacatccccg 11220ctcgcggtct ttaccctctt cgtgatttat ttatagctgg taaaggtaag atgattcttg 11280gttgtgatgg tgcaggactg gagttgcgtg tgttatcaca cttcatgaat gaccctgaat 11340accaagagat tgtactgcat ggtgacatcc atacacacaa ccaacttaag gctggtttac 11400ctaagcgtga catggcgaag acttttatct acgcattctt gtatggctct ggtattgcca 11460accttgccgc tgtatgtggt gtaactgaag atgagatgaa ggaggttgtt gcacggttcg 11520agatcgaact accatcactg gctcgtcttc gtgagaatgt catcgctgct ggtaataagt 11580ttggatacct gcaagcacct gatggtcatt gggggcgcat ccgtatgagt ggtggtgagc 11640ttaaagaaca caccatgctc aacgtattac ttcagatgac aggctccttg tgtatgaaat 11700atgccttggt taaagccttt gcagtcatgc gccgtgaagg tgttgcactg gataacctgg 11760ggaatccgtg tggcgtggct aacgtacacg atgaaatcca gatggaagtg ccagaagagg 11820aggtgttata ccttgactat gaattacctt tcacgttgga aggtttcgaa tctgagaagc 11880aagctatcaa agctgtgttc gaccctgaag agaagcgcgt acatgtggat tccgaagggc 11940gcatgtggtc tgctgctaac ttggttgaag tggatactgc tgctggcgtg ctgcgttgtc 12000agcgtcgcta ccacagggct ggtcatatta tcgctgacgc catgacatgg gctggtaagt 12060acctgaatat gcgctgccct atggctggcg agtacaaaat aggtgcaagc tggaaggaga 12120cacactaatg caaactgctc ttattattct tggagtcata ttatttatgg tagtgttctg 12180ggccttctct ggtattgacc cagattacga tggtaactac gactgagtta tactcaaggt 12240cacttacgag tggcctttat gaataactta actggagatt attatgatta aatattgctt 12300atcaattaac taaagaccgt aagataggta ttgaggttaa agcctgggac gcgggacaca 12360tctctgtagt tatagagtgc cgccaagaca atggtatgct gttaagaagc taccgttgct 12420tcaccaaatt acgctgcaaa gatttaactg aagaattatt cttacgttgt attgttgaat 12480ctattaaact tattagacct tacgctaagc aagttgtagg taatgtcaca gtggtaaatt 12540gatttaggtg acactatagg aggaagacct aggtaatcta ggtttataat gtagtatagg 12600taattaagta aatataggag atataaacat gtcaatggta actactctgg tattcgtggc 12660tcaatacttt cgtggtctgg ctaataagtt caagtacaaa gctattgaag ctattgagga 12720ccgcatcgaa gcagtacagg cagaacaagt tgaagttgaa gaacatcgta gttctcaaat 12780gattgactgc cataatcgct attacgcatc tcgtgatgac cttaatgcac gacaagtcaa 12840agaggtcgaa gagatgatgg cacgtcacca gcaagagcgt gacaacctga aggctgactt 12900tgaagagcgc aaggcatcca ttgcccttgt acatcaagct gcatctgaca gcctgaagaa 12960agagattgtt atgctggaag tggagttaga caatctgacc aaataattag gtgacactat 13020agaacaatag gacgtgggtt tgtcggagac agtaaatcca aggtgctcag tgagcgtaaa 13080gcctaagcac gtcctatgat tgtaaagtgt tgaacctctt gtgcatcttg cacaacccga 13140tacagtatcg ggctttctag tgagtacatg cttgtgctca gtacaaagct aacaaacaac 13200aggaggaata aattaatggc tcgtaatttt gattttggtg ctgaggttgc tgctgctact 13260ggtggtgtgt ttaagaatcc agaagttggt gatcacgagg cagttatctc tggaatcatt 13320cacgttggtt ccttccaaga catctttaag aaaggtaaca ctaccgaggt gaagaagcct 13380gctaacttcg ttcttgttaa ggttatcctg atgggtgacg atgacaagaa cgaggatggt 13440tctcgtatgg aacagtggat ggctgtgccg ctcaagtctg gtgacaaggc gacgctgacc 13500aagttcctga atgcagttga ccctaaagaa ttactaggtg gtttcgatga cttcatcggc 13560gagtgcatga ctgtgagcat ggttggcgat gagaaaggtg gcaagaatga tgacggcacc 13620ttcaagtacg ttaactggaa aggcttcggt ggtatgccgg ataaattgaa gaagctggta 13680ctggctcagg tagaggatga aggtctggaa atgactggtc acatcacctt tgacaagctg 13740accaaagata tcatcgactc tattcctgca caccttgtac gtcagtacct gctgaacgag 13800acgccgcgtg gtaagaacct gtcagtagtt ggttctcatg tagagggtat cattgccgaa 13860gcacgcgcag cagaccctga gtggaagaag gccaagaaga aagacaatga ggccacccct 13920gaagaccgca agacgctgga cactggcgct gctgttccgc aggaagtacc ggaagcgcag 13980aatgccccgg cacctgctat ggatgaagat gctgaatatt aatcaaggag gtttaatgaa 14040agtagaagca gtaaccctac acttcaagcc cggcgtaacg tcgctgggcg gcacgcagtt 14100catttctttt agcgagggca aggcctacca agacctgcac tatattaccc gtgaggggca 14160gcacgtcgtg aattacagcg accctgtgac aggcaaacgt cacggcattg gattccctat 14220gacggacatc cgtcagacca atacgatttt gtaagtctaa cgcgttggac aaatctgtgt 14280ctcttattta ggggacacta tagaagagag aattttaatc ggcgataatg ccacaattaa 14340cagaaggaga atttaaatat gttcactatc gaaactatcg taaaccgtgt tgttaaaggc 14400gctaccttgg tatccgttga gtctttcatt atcgtcgatg aagctggctc gctggtagct 14460ggcaccaaag catacgacac ccgcgaagaa gctcaagcta agattgacag catgggtaac 14520tttgctactg gcctggagtt tgctcgtgct tgcttccctg agcaggctga caaagcacag 14580attggtaagg ctaacattgt agctgaatat ctggattgga ttgctgctgg taagccagtg 14640aaagaagtta agtctgctga agaagctgaa gctccggcag tggaagctgc accggaagct 14700ccggttagcg aagaagaaga gttttaattg atgccctgtc tgccttagtg taggcagggt 14760cttttgcgta atagttattg gagaatgaat tatgccgact attagatctc gtttagtagc 14820agattatgtg tatggtcgtg atgtcaaaat gatgaaagat tacctcaaag ttattatctt 14880gcttgatggg gagttgtttc atactaaaac cttcaccctt cctgagttat ttgacttagg 14940atattggggt tatacctatc aggccatagc aaataaggtg ctactcgatg tattaaagga 15000gtggcctaca tgcgaccaaa cttcaacttc ggagctacag tatcggaaga caataatctc 15060atcctgtggc cgactgaagg taagagaatc gctctcatag atggagatat gattccatac 15120atcattggtt atactatcaa tgagatgaca cttgtccgag cgatgacccg cgttaagtca 15180gggcaagtag agcgcatcga agatacacct gagtgtaagc aagcttgcga ccgtgtaaac 15240tctatgctta actcttgggt gtatggtgct gaatgtgatg ccgcacgcat cttcctcacc 15300aagtcagata ctaacttccg cctacgcttg gctttcacga aaccatacaa aggtacacga 15360aaggcagaca agcctccttt cttctatgag atgcgacaac acctgataag tgtgcatggt 15420gcagaactgg cagatgggga ggaagcagat gacttgatga gtatcgcaca atgggatagc 15480cacaaccgat tcttgcaaga agtaggtaac gagttctcaa taggaagccc tgagcataag 15540gtgttctccg ataccgttat tgtatctgcg gataaagacc tgatgatagt accggggtgg 15600cacttgcagc cgggaagtga aatgaagtgg gttaaaccta tgggttggct tgaccttcgt 15660cgtaagaata acgggcaggt caaagacctt aaaggtgcag gactaaagtt cttctatgca 15720caaatgatta taggtgacga catagataac tatgcaggca tcccaggacg tggggccaag 15780tacgcttatg acctccttga tagttgcaag actgagaagg aactctatat ggctgtgctt 15840ggtgcctaca agtctaagtt tggagaaggg ccagtcaagc tcaagaacca tagaggaacc 15900taccgcatcg gcaaggcttt tgatctgatg ttagaatgtg gccgcttggc tcatatggca 15960caattcaaag gtgacatctg gcgtgcggat aagaatccaa ttgtgtgggg agatgatgat 16020tcatggcaat cagattgaag gcttcggagg tagctgacta caagaaagag ctactagaga 16080agcagaaatg gaagtgccct ttatgtggcg gcagcctcaa ggctgtcact gcaattaacc 16140gtgtacttga ccatgaccat gagacaggct tctgtcgtgc agtggtttgt cgtggctgca 16200atggtgcgga gggtaagatc ttaggtgtta tttctggtta tggtaaggca ggtaacaatc 16260gctacttcca actgaagtgg ctggagaact tgtatacata ctggaagtta catcaaacac 16320ctcagacgga taagttgtat cataagcata agactgaggc ggagaagcgc gaggctcgca 16380atcgcaaggc tcgcttggca tacgcaagaa agaaggaggg taaagttggg taagctacgc 16440tcactgtata aggactccga ggtacttgat gcaatagagc aggctaccga cgagaaaggt 16500aatgttaatt ataacgagat ggctcgcgta ctttctgcgc atcctgtcgg caagaagatt 16560acacggcagc ttgctcgtta ctggcatggt caattcatgc ataccaagaa gaacggtgac 16620tactaccaga ctctttctca ggaggatagg cgactcaaag aagcacgtaa gctcaggact 16680cctgaccgct atgaggatct ggctattgta ccattgcctg actcgcctca tagaagtgta 16740ctggtgatcc ctgataccca tgcaccttat gaacacccag ataccttgga gttcttggca 16800gcagtggcgg cacgcttccg tcctgatacg gtggttcact taggagatga ggcagacaaa 16860catgccttgt cattccacga tagtgaccct aaccttgact ccgctggtgt ggagttggag 16920aaggcacgtg ccttcatgca caagctgcac cggatgttcc cggtcatgcg cctgtgccac 16980tccaatcatg gttctatgca cttccgcaag gcaagcgcca agggcatccc tgtccaatat 17040ctgcgcactt accgagaagt cttcttcccg catggtggcg gcgaccaatg ggattggcaa 17100cacactcatg tcctggagtt acctaacggg gagcaggttg cattcaagca tcaaccagca 17160ggttctgtgt tagcagatgc ggcacatgag cgaatgaatc tggtgtgcgg ccacttgcat 17220ggtaagatgt cagtggagta tgcacgtaac acacatgagc aatattgggc tgtgcatggt 17280ggctgtctta ttgacgagtc gtctcgcgca tttgcttatg gccgtgagtc caagtataag 17340ccagcattag gttgtgtggt gattgtagag ggtgtacctc agattgttcc aatgcagacc 17400aatgcagaag gtcgttggat tggcaggatt taagtgacac tatagaacaa agggtcaggt 17460aatacttatc ggctggcata tccaaatgat attgcactgg cccttgattg tatagtgaat 17520ggaggaatta attatgtcag aaattgatat tggtaagtac gttgtacgcc gtgcagctta 17580tcgagatgcc ttctggaata aactgtgtga aagcttaaac aagcaaccag atggggtgtt 17640caaagtgtcc agtgtagaac ttaactacaa ctctatcatg ttagaaggtg tggagaaacg 17700cgaatggtat gcaccttatt tccaggtcgt tgactccctg caaggcgaag agtccaacat 17760gttggacaac aacatggtta ctaagcctaa gcactatgag ttcttcgagg gtgtcgaggc 17820aatcactatc attgcccgta gcatgaccga gaagcaattt gctggttact gcatgggtaa 17880tgcattgaag taccgtctgc gtgcaggtaa gaagttcaat actgaggaag acctgaagaa 17940agcagactac tacaaagacc tgttccagaa gcatcgccat gaatgtattg atgaggatct 18000ctaatgaata tcttccaatt cctaggttta cctgaagatc atcgttccaa acctgttatg 18060ctggttaagc acagggatga agtgccagaa agcaaactta cattcccggt ttatgcacaa 18120gtgaaaagag atggaatatt tagtgctaca gttgtgcgtt ctgatggtac tgtgggtatc 18180tttggtcgca ctggcaaaaa gctggttaat gtagaacaac tggaagcgtc ttttataggg 18240tggcctgctg gtgtctacct cggtgagttg caatctatgg ccgttgatat ctaccttgag 18300gcgctttcgg gtgtggtgaa tccaaacagg actgagcctc ttgacttcat aggacagcag 18360attaaagata acctgtacat tgacttcttt gatatgctga ctattaaggc attcatcgaa 18420gggcagacgg aggttacatt cttaaagcga tatgaagctc tatgtcgcag attgaaaggt 18480tgccttccac ctgagaatgc aatcctgact atcacacctt gccacaccga gcaagaggta 18540gaggcgtttg cacagaagca cattgatgcg gggcgagaag gtgcagtctt taagttagac 18600tgtgactatg aagcgggcca caagggcttc cgacagacca agattgtacg catggtctca 18660tacgacttaa cgtgtattgg ttgggaagag gggaaaggta aatacaaagg taaagtagct 18720aatcttatat ttaaatggaa gggtggcaag acaatcaagg ctatgcttgg ccgtggctgg 18780acacatgaag atgccacccg tatgtatcac gatattaaac acggtggtga actgaacgtc 18840atcgggaaga tattcgctat caaggctctc caagaatcta gcaagggagt cctgcgactt 18900cccaaggttg gagagttgcg ccatgacaag gaggagcctg atgtcttttg attcaatgaa 18960agcgacaaag gcagttgagg tagcagaagc tatctttgat atgctgtctt gtgggattga 19020agtcccttat acacttctgt ctgatgcaga agatttaggt ctgtctgtgg aagctatccg 19080cgagaaagtg gaggagttgt atggcgacga ccaagaagcc gactatcaat attgaaggtt 19140gggatatgct ggagaaaatt atacttgctc catcaagacc tcgaccggat aagtcacacg 19200aagagttagt atgggatgaa gccaagcgct atatcctgtc ttgtatcaag cagcagtttg 19260tggtgcagcc atgataaggc aggcttgctt cctagatatc cctgagataa ttaatctagg 19320gaacaggtat gtagaagagg aagtcaaggt agttaagcat cattcagcta catgggatgc 19380agatcaaagc gcacatcacc tttgtgcatc ccttaccagc aaggatttat ttctatgggt 19440ggctgtggaa gatggtgtta tcataggttt cctgtgggcg gcggctcaca tcatggcacc 19500ttggtctccg gcacttgtgg cttctgatct actattctac atcataccag aaaagcgagg 19560gtctcttgct ggtgtgcgct tgctcaaagc ttacaagtct tgggccaagg agcgcggctg 19620catagaggca aggttgtcta tcgcatctgg tatcaatgag gaacgtgtgg ggcggatgta 19680tagtcgatta gggtttactc cgttcggtac agtgtataac ttgaagtttt aaggagataa 19740catgggtgta gttaagaagg catttcaagc agtaggtctg gcacaaaagg cacctcgcat 19800tgaggcagct aaggttccag cacaacaact tgagcggcag actgaggtta aatctgaaga 19860catccagatt ggacaagagg atgatgctgc ggcatctgct aagggcaagc gtggccttgt 19920gcgccctgta gcctctagct taggagtttg atatgcaaga cactatactt gagtatggtg 19980gacagcgatc gaagatacct aaactatggg agaagttttc taagaaacgc agtccctacc 20040ttgacagggc aaagcatttc gctaagttaa cactcccata cctgatgaac aacaagggag 20100acaatgagac ctcgcagaat ggttggcagg gtgtaggtgc acaagctacc aatcacctag 20160ctaacaagct

ggcacaagtg ctattccctg cgcaacgatc attcttccgt gttgatttaa 20220cagcaaaagg tgagaaggta ttagatgacc gagggctgaa gaaaactcag ctagcaacca 20280tcttcgctcg cgtagaaacc actgcaatga aggcgctgga gcaaaggcaa ttccgcccag 20340ctatagttga ggtgttcaag cacttaatcg tagcgggtaa ttgcctgttg tacaaaccaa 20400gcaaaggtgc gatgagtgca gtaccaatgc accactacgt agtcaaccgt gacactaacg 20460gcgacttgat ggatgtaatc cttctacaag agaaagcgct acgtacattc gacccagcaa 20520ctcgcatggc aatagaggtt gggatgaaag gtaagaagtg caaagaggat gataacgtca 20580aactgtacac tcatgcgcaa tatgcaggtg aaggtttctg gaagattaat caatctgctg 20640acgacatccc ggtaggcaag gagagccgca tcaagtccga gaagctacca ttcattccac 20700ttacatggaa gcgcagttat ggcgaggatt ggggccgtcc cttggctgag gattattctg 20760gtgacttgtt tgttatacag ttcttatctg aggccatggc ccgtggggct gcactgatgg 20820cagatatcaa gtacctgatt cgacccggtt cacaaactga tgttgatcac tttgttaact 20880caggtacagg tgaggtcatc acaggtgttg cggaagacat ccacattgtt cagttgggta 20940agtatgcaga cctgacacct atcagcgctg tgctggaagt atacacccga cgcatcggtg 21000tcatcttcat gatggagacc atgacacgcc gtgacgctga acgtgttact gccgtagaaa 21060tacaacgtga cgcgcttgag attgagcaga atatgggtgg tgtatattcc ctgtttgcca 21120tgaccatgca gacacctatt gccatgtggg gcttgcaaga ggcaggtgat tcattcacta 21180gtgaactggt agaccctgtg attgtaacag gtattgaagc actaggccgc atggctgaat 21240tggataagct ggctaacttt gcacagtata tgtccttacc tcaaacatgg cctgaacctg 21300cacaacgtgc aatccgatgg ggtgattaca tggattgggt gcgtggtcag atatctgcgg 21360aactcccatt cctcaagtct gaggaggaga tgcaacaaga aatggcacag caagcacagg 21420cccagcaaga ggccatgctc aacgaaggtg tggctaaggc cgtaccgggt gttattcaac 21480aagaaatgaa ggagggttaa ttagtggcct ttgaatttgt agaaccgacc aatgaaacta 21540ccgctgctcc ggctgctgaa gagaacaagg aggtgactaa tgatgttgct ggtgttgacg 21600ctggtaatac tggcattgac gtacagaatg gtgcagatga tcaaggcaat gaggacaccg 21660gaggagaagc tgttggacag ccttcaggag agggagatgg tgaaccggat ggtaaaccta 21720agccagatgg ttccacggat gaggaagcgc gatacttctt cggtgaacat gaagtaatca 21780ttgaagtgcc tgatgatgtg accgaagctc tcaaagagaa gggcatcgac gctatgcagg 21840tggctcgtga gttgtatggt gaaggtggta gatttgaact gtcagaagaa accaagcaga 21900aactgtatga tgcatttggt aagttcgcag tagatgccta cctatctggc ctcaaggctc 21960agaacgaaac ctttttcctc cgtgaagaaa ctgccgccaa ggaggcggaa gctgcaaacg 22020cacagcgcta cacggatatt gccaaggagg ttggcggtga cgaaggctgg agccgtctgg 22080aggagtgggc gcttgatact ctttctgatg aagaactgga agcatttaat gcagtgatgc 22140agtctggcaa ccaatatcta cagcagtacg ctgtgcgcga gttagaaggt cgccgtaagg 22200ctgcacaggg tgacgataaa cctaacctta ttgaaccaac ggctaccgct gctgcatcgg 22260aagataatgc acctctaagt cgggagcagt acatccgaga gattgcacag ttaggccaga 22320agtatggacg tgaccgcaaa gggatggctg aagcacaggc acgtctggat gcacgtcgcc 22380gcgcaggtat ggctcgcggt ctttaattgc ctatttaggt gacactatag aagggaggta 22440gtcctcccta acctatcaac ttgatttata aggagattat aatacatgtc tacgccgaac 22500aacttgacca acgttgccgt ttccgcttcc ggggaagtag atagtcttct cattgagaag 22560ttcaacggta aggtcaacga gcagtacctg aagggcgaaa acatcatgtc ctacttcgac 22620gtgcagaccg tcacgggaac caacactgtg agcaacaaat acttgggtga aaccgagttg 22680caggtattag caccgggtca gtctccggct gcgacctcta ctcaggccga taaaaaccag 22740ttggtaatcg atgccactgt tattgcccgt aacacagttg cacacctgca cgatgtacag 22800ggcgacattg atagcctgaa gccgaagctg gctaccaacc aagccaagca actgaagcgt 22860atggaagatg agatgctgat tcagcagatg atgttgggcg gtattgccaa cactcaagct 22920aaacgtacta acccgcgtgt taagggtcat ggcttctcta tcaacgtaga ggttgcagaa 22980ggtgaagcgc tggtcaaccc tcagtacgta atggctgctg tagagttcgc gctggaacag 23040cagttagagc aggaagtgga catctccgat gtggctatcc tgatgccgtg gcgctatttc 23100aacgtactgc gtgatgcaga ccgtatcgtt gacaagacct acaccatcag tcagtctggt 23160gcaaccattc agggcttcac cctgtccagc tacaactgcc cggtaattcc gtctaaccgt 23220ttccctaaat attctcaagg tcaaactcat cacctgttgt ccaatgagga taacggctat 23280cgttatgacc cgctcccggc aatgaatggt gctatcgctg tcttgtttac ggcggatgcg 23340ctgctggttg gtcgctctat cgatgtgact ggtgacatct tctatgagaa gaaagagaag 23400acctactaca ttgatacctt catggctgaa ggtgcaatcc ctgaccgttg ggaggctgtg 23460tctgttgtta caaccaagcg caacaccact actggagcag tagaaggcac tgatggtgcg 23520cagcatacta tcgtcaagaa ccgagcacag cgtaaggctg tctatgtcaa gaatgcggca 23580cctgtagctg ctgctgccgc tagcctgtct gctgaagatc tggttgctgc tgttcgtgct 23640gtgatggcta atgacatcaa gccgactgca ctgaagccga ccgaggaata acctatgccc 23700tatctacctt gcgtaggtag ggttcttttg tttaggagga ttcatgcctg taattcaaca 23760atcaagtgat gtaggttaca tcatgtccga tgcaagcttt agcatcattg atagcaagct 23820agaggccgtc aacctttgta tgcgggccat tggtcgtgag ggtgtggatt cccttgactc 23880aggcgacctt gatgctgaag atgcaagtaa gatgttggac attgtgtcac agcgcttcca 23940atataataaa ggtggaggtt ggtggtttaa tcgtgagcct aattggcgca tcgtgccgga 24000cactaatggc gaagttaacc tgcctaataa ttgcctagct gtcttgcaat gttatgcatt 24060aggtgagcgt aaagttccta tgacaatgcg tgcaggcaag ctgtactcca catggaatca 24120tacgtttgat atgagaagtc atgtgaacaa agatggtgct attcgtctga cacttctgac 24180atatctacct ttcgaacacc tacctactag cgtaatgcaa gcaatcgcat atcaggctgc 24240ggtggagttc attgtatcta aggatgcaga taagaccaag ttgaccaccc atcagcagat 24300tgcagcacag ctattcgttg atgttcaatc tgaacagatg tcccagaaga gactcaacat 24360gttagtacac aaccctacac agcgtcagtt tggtatcatg gcaggtggat ctcagaacgt 24420accagcttac tcgcattcac cttacgatgg tcatccactt aaaccttggg agagttatcg 24480ctaatggaag ttcaaggttc tttaggtcgc cagattcaag gcataagcca gcaacctcca 24540gcagtaagat tagatggaca gtgttcagaa atggttaaca tggtgcctga tgtagtggag 24600ggaaccaaat cccgcatggg tacaacgcat attgccaaac tcttagaata tggtgaagat 24660gacatggcag tgcatcatta ccgtagaggg ggtgaaggtg aggaggagta tttcttcata 24720atgaagaagg gtcaagtacc tgaaatcttt gacaaacaag gacgtaagtg tatggtgcaa 24780tcacaggatg cacctatgac ctatcttagt gaagtgacta accctaggga agatgtgcaa 24840tttatgacta ttgcagatgt gaccttcatg ttgaatcgca agaagatcgt caaggcccga 24900cctgaacgct cccctcaagt aggtagcact gctattgtct ttatggccta tggtcaatac 24960ggtacgcact acaagattat tattgatggc gtagtggctg ctggctataa gactagggat 25020ggtgccgagg cacaccatat tgaagacatc agaactgaaa gcatagctta caatctgtac 25080cagtcactcc aaagttggga taagattgca gactatgaaa tccagttaga tggcacctca 25140atctatatca caaggcggga tggctctact accttcgata taaccacaga agatggggca 25200aaaggtaagg atttggtagc catcaagtac aaggtggcat ctacagacct cttaccatca 25260cgtgcaccag aaggctacaa ggtgcaagtc tggcctactg gcagtaagcc tgaatctcgg 25320tactggctgc aagctgagaa gcagaatggg aacattgtct cttggaagga gacactggcc 25380gccgatgtgt tgatagggtt tgataagtca accatgcctt acattataga acgtacaggg 25440tttgttaatg gaattgcgca gtttaaaatt agacaaggcg actgggaaga tcgcaaagta 25500ggcgatgacc tgactaaccc tatgccttca ttcattgatg aggaagtgcc tcagacatta 25560ggtggtatgt ttatggtgca gaatcgtcta tgtgttactg ctggcgaggc tgtaattgca 25620actcgcacat cttacttctt tgacttcttc cgatataccg ccgtatctgc tgtagccact 25680gacccatttg atgtattctc agatgcgagt gaggtttatc agcttaaaca cgcggttaca 25740ttggacgggt ctactgtctt gtttgcagat aaatctcagt tcatccttcc tggagataag 25800cctcttgaga agtcaaacgt attgctcaag cctgtaacca catttgaagt taacaataat 25860gtcaagcctg tagctacagg tgagtccgta atgtttgcta caagtgaagg tgcttactca 25920ggcataaggg agttctacac agactcttat agtgatacca agaaggcaca agcaataact 25980agtcatgtca ataagttgct agaaggtaat gttattatga tgtcagccag tactaatgtg 26040aacaggctgc ttgtcttgac cgacaagtac cgaaacatta tctactgcta tgactggttg 26100tggcaaggaa ccgaacgtgt acaagctgca tggcataaat gggagtggcc tttgggtacg 26160tttatccgtg gcatgttcta ttcaggtgag cacctatatt tgctcataga aagaggcagt 26220actggtgtgt atcttgagcg catggacatg ggtgatgcgc ttgtatataa cctgaatgac 26280cgcatccgta tggataggca agctgaactt atctttagac atatcaaggc agaagatgtg 26340tgggtgtctg agccgttacc ttggcaacca accgatgtaa cattgcttga ctgtgtactg 26400atagatgggt gggactctta cataggcggg tctttcttgt ttagctataa cccaggcgat 26460aacaccttaa ctacaacctt tgatatgcac gatgatgacc atgtgaaggc taaggtagta 26520gtcggccagt tatacccaca agagtttgaa cctacacagg tagtaatacg tgataaccaa 26580gagagggtgt cttatataga tgtgccaacg gtggggcttg ttcacctaaa cctagacaaa 26640taccctgact tcaaggttga ggtcaagaat ttgaagagtg gcaaagtacg taatgtgctg 26700gcctctaaca gggtgggtgg tgccataaat aatattgttg gctatgtaga gccgagagaa 26760ggtgtgttca aattcccact aaggtctctt agcaccgaca cagtttatcg tgtgatggta 26820gaatcgcctc ataccttcca gcttagggat attgagtggg aaggttcgta caaccctact 26880aagaggagag tgtaaatggc aataggtact gctcttacag caggattgtc cagtgtagca 26940ggtagtgctg catctggtgg tttcctgtct tcgttgggtg gtgctatagg tgcagaaggg 27000gtaatgggtt ctgccatgag tttcttaggc ggaaccactg gaggcttctc taatgctggc 27060ctcctgtcgg caggtatgca aatgcttaac ccgataggag actacttcac gcagaaagaa 27120acagcgaagg cgatgaagaa ggcgcaagat gagcaatggc gtcagcagtt gatagccaca 27180agggaggctt atgcttccgt ggctaatgct gaaaggtctg cctctaagca ataccattct 27240gaactaatag acaatcaggt atccttatta cagcaacgag cacaagttgc cttgcttgca 27300ggtgcgagcg gcacaggtgg taactctatc acctctatgc tgaatgacct gacaggtgaa 27360gctggtagga accaagccac cattattgac aactatgaaa cacagcagat taactttgct 27420aaccagctca agtctatcca gaaaggtggt cagatgatga tgcgctcctt tgagaagcca 27480tctgcattca gtgccatagc caaaggtgtg tctggtatag gtgaggctta cctgtctggt 27540catcagaagg gtacagcact tagcaaggct tggtctgact ctaggacata ttcatcagga 27600acaagaggag tttaaatggc aattgaacgt caagctgtac agggcttacg ccgagtgcag 27660tctactggtg ggccaagtgc tgctagtttt gcgactcgtc aggttggggt gcaagagact 27720agtgcatctg gtagccgctt tcttgaagac cttgtaaatg ctgctggcag tttggcgact 27780gtcactactt ctattctgaa ccaaagagtg gaagatgata aggtaagaca atataatagg 27840gcgcttactg gcctaatgcc aactgaagat gcaacggtag gcggcgcacg cgcacacatg 27900cttgttagtc tacaaaatga catcatcgcg caaactatgc aactgtccga tgatgcacaa 27960cgctttgatg gcgatgacag tcaatgggaa gatcacgtca ttaatgcccg catggctgtg 28020caagaccgcc tatgggatac ctaccctgaa cttcgtggtg ataaggagtc catgcgggta 28080gttactaatg ccttcatgga gcagcaacct aaaatatttg cagcaaggga gaccgccaag 28140ctgaagcagg aggcggaagc ccgcatcaag tctatggagt cacgcattct gctggctacc 28200cgtgatgttc ctggcgaagc tatgggtgat gccttgaatc agttgcagaa agaagctatg 28260gctatgcaaa tcaccaagca ggagtttgat gcactggttt ctcaattggc agctaatcgt 28320gcagctattg gtgatgattc tatgattcaa ggaaccaagt ctcttaagga tgagaatgga 28380gtatcactct atgaccgagt aggtcagtta cagacaggag agattcaggc caaccgcaca 28440tgggcggcgc agaaccaagt ggcactcttt gagaagaagg atgctgcaat caaagccttt 28500gaagctggac agcttaaccg cgaacagcta cttcaggtca tgcagaacca caatgaaatc 28560tcaggaggca ccgcttggtc tgatagcgag atcaaatctt tatttgatag acaggctaag 28620gctcgtgcta cgtctgccaa gctggaagat ttggtggccc gtggtgaaca tggctcaccc 28680ctaggcttgc aagatatcag taaggaagac cgcaaagcgt atgctggtgc attggttgat 28740gcctacacca agttagccaa tgacgagata acccgtacag gagctactgg tgaagaagct 28800gaagctatcc gtggccgcta tgagcagatg cgatatgcca agctgggcca gcagttgatt 28860gaagacccta tcattaaaga acggtacggc tcgctgatgc aactctcttc tgccaacctc 28920aaagatatga agattgaacc tgaagcattg cagactatta tgcgcgcccg cgattctatc 28980ccggaagatg cccgccgggc ggtgatgggt gacaaggagt acgcctttgc ggagaattat 29040gatttggcga cacgcatggg ttacactcct ggacaggcta tagagtttgc acagaatgca 29100tcgcgtgggg acaagcttcc cggttctgtt atgaaagaat tgaatgatga agtagatggt 29160gtggttagtg atgttgcgag cggtagctgg cttacgcgtg gcgacaacat gagtgacatg 29220ggtcgtgacc ttatgctaga agaggcaaac cagattgctc gctctatgaa ggttgcaggt 29280cataacaatg acaccattaa gcgtcatctc aaatctttcc tacagaatca gtacactcag 29340ctatctgaag gtttcttcac tcaaggtgtt ctggtcaaag gtgatgtgag gacgctaggt 29400gacactatag gtgccaacca aaaagacgta cctacggtat tacgtcagta ccttgacaat 29460cataagcaag cattgctgga tgcatctggc ggtatggaag aaggagactt atactttgat 29520gtagactcta agcgcggtat gtttacaata cgtgctggtt ctgggcgtgt gccagttact 29580ccagctatgc ctttgtctga aatcaaggga caggacttac tgaaggagca ctacgagaag 29640gcagttaaag agcgcgatga agcgaagaag aactttgaag ctaatcagat gcgtatgtgg 29700ggtgctggtg gttaccaatc tcctgcacca gaaaagacta cagctaagac tgtaggttcc 29760cgtggcatcg ctgacttcct catgtcgcct gcctttgcat ccggtgagaa tctaccttcc 29820aactttgaat tcaactacaa gaggaataat atggacttct acaattatgt agctaagacc 29880gagaatgggg ccaacgtagg gtttgaccga gtagctggcg tgtacactcc gtacaaagat 29940gcacacggtc agtctgtagg ttatggtcac ttcctcacgg aggaggagaa gaagaatgga 30000tacatcacta ttggcgaaga taaagtacca tttgcaccgg gacaatctca gttaacacct 30060gagcgggcaa tgcgtctgct tgagcaggac atgaagagcc acgtacctag cacaaaggat 30120tgggctgtac cttttgatgc aatgcatccg ggagtgcaac gtggcctcat ggatttatct 30180tacaacttag gaaaggatgg catcaagaat gcaccgaaag cctatgcagc cttcaaggct 30240ggcaagttca ccgatgggtt tatcgagatg ctgtctactg catctactga aggtaagcgt 30300agctccggcc tgctagttcg cagggcggaa gcttataacc ttgcacaaag cggagggtct 30360gtacctaaga ttagcgaagt tgagacaagg gaagatggtt ccatgtacgt taagttctca 30420ggtagcatgt cagaagcatt tgtgagcaag tctatccttg gtaagatagg taaagatggg 30480tggatggaag tctaccctcc taaagcagga gcacttgcaa gcggcaccaa agtgggtcgt 30540attaaactgt agtgtcatac tcaaggttgt ctaacatgtt ggacagcctt tatgaatgac 30600attaactaag gaggtaacat ggctgacgat attagccaaa gctgggtgac ggtatctcaa 30660cgcaggttgc cgcctacctt tgcacaagtg gcagaagccg agcgtaagct tgaagaacaa 30720agagctagcg ataaggttat gcagactgca ctggaaagcg aatgggcgct atacggtggt 30780cagcgtgcta ttgagcggca tacaactgag tttgccgaac aagaaggcta cacggttcct 30840gagtcaacaa aagatgaact atcaaagatt catggttttg aaattgcaca ggatattgtg 30900aaggatgtta agtcaccaga agagttgcag tttcgtatgt ccaatgctat ggcggataag 30960gagcgatcgg agatccttgc acgtaatggg tttacagggt ttagcgctca gttagctgct 31020ggtatcttcg acccagttgg ttgggctgcc tctatggttg ccgcccctgt agctggtgca 31080gtaaaggttg cccgtgtcgg tcgtatcata aagacggcag cagtggctgg tgccgagaac 31140gcagcattgg aagccatcct agccagcggt gattaccaga agggcgcaga tgatgtgctg 31200gctgctgctg gctttggtat gataatgggc ggcaccattg gcgcagctac acgcgaacgc 31260atcgccagaa agccaggagt acaaggagtg aatgacggtg ctgagaccgt agtggatgac 31320ttagatacgg tcgtaaaggg agcagatgag tttgatgcat ctgcggctaa ggctgtacga 31380gaggctatgg agtatgacgc gtacatggct gtgcgttcct atgaaccact gagggctaag 31440gaagtggata tggatgtagc aatcctgtct cacttagatg acctgaaggc taactctagc 31500gtgcgtatga gtgcctccga gaaaggtaaa ctgaaggagc agatacgcca gcttgaaaca 31560gaagccgcca ccattaaagg caagaaggta gatgccgtgg cagaagctgc tgctgctaag 31620ggtgcgccta agtctgctgc tgataggcta gacttggatg ttaagaagaa ggcactggca 31680cgtcgctttg atgagccgct tgccgacatc caaacaagac tcgacgaact taatgctaaa 31740ctggcccgcg tggagaacgt aggtaagtca aaggaggagt tgaagagatt ctctaatcta 31800actagggagc agcaaatcaa ggagctaggg ttagatgctc cggctcgtaa agttgagatg 31860acaagtgcgg tacgggaggc tcttgcagct atacgtgctg agaagaagaa gacacccact 31920cagactcatg ccgaagccaa agcacaggca gaagaggaag tgcggcagaa gcgagatgac 31980tctatcggcg ctaagcgtgt agaggattct gaaattgcag gtgaacaatt tgacctgtct 32040gatagcatgg aagatcttat ggatgacctt gcacgtgaag catatcagtc tgaagttaga 32100cctgtaaacc tcaagggact tggttctgta tcttccgtga ttctgaactc aaagaaccct 32160gtgtttcggg gtcttggttt gcgactgctg gagaatgcac aaggtggtgc ctaccaaggt 32220aagaccgctt ctatcttgtc taacgtgtat ggtaacttga ttcgctttgc tgagaagaat 32280cgatacaatg atggcttctc tcaattcatc aaggataaca atttacgtgc tgttgattac 32340ctgaaccctg ctgttacgag ggattttaat aaccagattt atactgctat tgtcaaagga 32400atacctgatg acacgccacg tggtgttaag cttgctgctg aaggcatcgc agataagctg 32460gctaagtctc ttgaaatcag aaaggctgct ggtgagaaag gcttcgaaga tgtcaagtcg 32520gcacgtgatt atatccctgt gatatatgat ggtatcaagg tgactgaagc agtcaataga 32580cttggtagta gcgaggctgt tattgccctg ctgtccaaag gttatcagac tggtaagtat 32640aagatgggta agaaggcagc ggatgcactg gctaaggtgc agtatattcg cgcctccgat 32700tctaccttat caagccgtgt agcctttgac agggtagttt ctcagcagca acaagcacag 32760cttattgaag acctgaagag agcaggtgtg cctgataata tcatagataa cttcatcgaa 32820ggcactgagt tgcaagagat ggcggaatca gtgtctaacc gagctaaggc aagcatgggt 32880atcaacactc aggctgaata tggcgggatg aaggttcagg acttgctcaa cactaacgta 32940ggtgagttgg cggagaacta cggcaaggag gcagcaggtg gtgcagcttt ggcggctatg 33000gggttcccga cccggcagtc tgtactgaat gcaattgacg cagcagaacg cgcagggcgc 33060aatatggcgg gcgctgacgc caaggcaatc aaacagctta gggcggaatc agaaatgctc 33120agggactccg tgaagctcat atacggcaac accattgacg caaatccaaa tgcgggtatc 33180gtccgaggga ctcgccgtgt acgtgagatc actggccttc tgcgtttggg tcagatgggc 33240tttgcgcagg tgccggagtt ggcccgcgcc attaccaaga tgggagtagg tacagtgctg 33300aagtctatcc ctgccactaa gttcttacgc tcccgcgccg ggcgtaaggg cgggacagca 33360caaggtgagc tacttgagcc ggaactgcga gagatggaag aactcatagg ttatatcggg 33420gaagataact ggctatcagg ttggaacgta aggcacgatg agttcggaga gaccgctgac 33480aacatgggac gtctgtctgc catcatcgac aatgggttgg ctatgggtag ccgtattaac 33540acatggctgt ctggcttcaa ggcgatacag ggtggttctg agaagatcgt agcacgctct 33600atcaataagc gactcaagca acatttgatg ggcgagcgag agctacctaa gcgtgacctt 33660gaagaggttg gcttggatga ggctaccatg aagcgactca agcgccactt tgatgagaac 33720ccgatgtatg ccgactataa cggcgagaag gttcgaatga tgaactttga cgccatggag 33780ccagacttac gagaaatcgt aggtgtggca gtgcgccgta tgtctggtcg tcttattcag 33840cgtaacttca ttggtgatga aggtatctgg atgaacaagt ggtggggcaa ggctctcact 33900cagtttaaat cattctctat tgtgtctatt gagaaacagc ttattcacga cttgcgtggt 33960gataagattc aggcagcaca gattatggca tggtcttcct tgctaggttt tgcatcatac 34020gctacacaga tgcagatgca ggcgattgga cgagaagacc gagacaagtt cttacgggag 34080aagtttgata ctcagaacat agctatgggt gtattcaata aactaccaca agtggctggc 34140tttggcttag ctggggatac ctttgcaaca ttcggcctta tgccggactc catgatgcag 34200gcaccgggtc gtatgggctt ccgtcagcaa ggatttggcg acttagtggc tggtgctggt 34260gtcataagtg atgctgtgaa cttgtcacag gctttagtga agtatgccaa tggagatgat 34320gatgtctcca ctaggcagtt agtagataag gtacgacgtc ttgtgccttt ggcaaatacg 34380attggtgtag gtcagatgac caaggccagc gtagacttat tggaggattg atgagttata 34440ctttcacaga acacacagcg gtaggttctc agacgactta tccgtttagc tttgctgggc 34500gcgacaaggg ttacattcgc gcatcagata ttattgtgga agtgtttcat gaaggcgagt 34560ggagtattac acaaggttgg gtgctatctg gcactcacca gattaccttc aatgtagcac 34620taccagcagg gactaagttc cgcatacgta gagatgtaga caaagagtac ccttacgcgg 34680agtttgatag aggtgtggct cttgatatga aatcattgaa caactcattc attcatatct 34740tgcagattac acaggagatt cttgatggct tctacccaga aggttacttc gtcaaacaga 34800atgtgtcttg gggtgggtat aaaattactg acctagctga tggcacaaac cctcacgatg 34860cagtgaataa ggggcagctt gacgcaatcg acaggaagca tactgagtgg aatgaacagc 34920aagatattgc aattgctgga ctcaaggcag ggatgacatc aggtctctct catcggacag 34980taccttgggt tacagtagcc gccgggggag agcaagttat taggcctcct tacatctttg 35040aatccgcctt ggttttcctt gatggggtct tgcagcacga actgtcaggt gcagttacta 35100tagctaacag caccctcacc ttctccgagc ctctacgtcg tggcacagaa gtgtatgtat 35160tgataggtag tcgtattgca acctcttcac cgggcctgca tatggagttt aataaagact 35220taggtgcagg

gactacggag gttaggattg gtatggcttt ctctcatatt gatatctacc 35280ttgatggctt gttccaacct aagtcaacat atcaaataaa cggcgatctt gttacattct 35340ctgagggtgt accagcttgc catatgtcag cggatgtagt cactttatag gaggtaagat 35400ggttgattcc gaactggtta gcggcgggat gaagttagcg ccatctgcct tagtatcagg 35460tgggtacttc ctcggcatca gttgggacaa ttgggtactg attgcgacat tcatttatac 35520tgtgttgcag atcggcgatt ggttctacag taaatattca ttatggaagg agaagaagcg 35580tggcaaaaca caataaacac gcagctactg aagacgaggt aggtaagtta catagtgcta 35640tcactaatct tttcaataag aaagctgctg caatcctagc tgcggtagaa gaagatcctg 35700atgcagcaat tgcactggtt tccggcaagg acatgggtgc catgtgtaag tgggtattgg 35760ataatggtat tatggctaca cctgctgcac agcaagaaga gtctgcacta tctaagcgcc 35820ttgctaagat caaagcagca tctcaaggta aagtaatcca atttgctaag gaggcttaat 35880ggctagagca agggagtcac aagctgaagc ccttgcccgt tgggaagccc tgcatgagtt 35940acagcaaact tttccgtaca ctgtagcagg gctactctca tttgctcagg ttgtaatcaa 36000taatttaatc actggcaatc cagacctgaa ccgggtacaa gcggatattc tgaaatttct 36060ctttggaggt aacaagtatc gaatggtaga agcacagcgt ggtcaggcta agacgaccat 36120tgcagctatc tacgctgtgt tccgtatcat ccacgagcca cataaacgta tcatgattgt 36180gtctcagaca gcgaagcgag cagaagaaat cgctgggtgg gttatcaaaa tcttccgtgg 36240tctggacttc ttggagttca tgttgcctga tatctacgca ggtgacaagg ctagtataaa 36300aggttttgaa atccactaca ccttgcgtgg tagcgacaag tctccatcag tggcttgcta 36360ctccatcgaa gcaggtatgc agggtgcgcg tgcagatatc atcttggcgg atgacgtaga 36420gtcgttgcag aactctcgta ctgccgcagg tcgtgcttta cttgaagacc ttaccaagga 36480gtttgaatcg atcaaccagt ttggtgatat catctacttg ggtactcctc aaagcgtaaa 36540ctccatctac aacaacctcc ctgcgcgtgg gtatcagatt cgcatctggc caggtcgcta 36600ccctacactg gagcaggagg cttgctatgg ggacttccta gcgccgatga ttcgtcagga 36660catgattgat gacccaagtc tgcgctcagg ctatggcata gacggtacac aaggcgcgcc 36720gacttgtcct gaaatgtatg atgacgagaa gctcattgag aaggaaatct ctcaaggtac 36780agctaagttc cagttgcagt tcatgctgaa cacgcgcttg atggatgccg accgctaccc 36840tcttcgtctt aatcagctta tcttaatgag ctttggcact gacgtagtgc cggagatgcc 36900gacttggagt aatgattcgg taaaccttat cagtgatgcg ccacgcttcg ggaacaagcc 36960cacagactac ctgtatcggc ctgtgccgcg tccgtatgag tggcggccta ttcagcgtag 37020gttgatgtat atcgacccgg caggtggagg taagaacggc gacgagacgg gtgtagccat 37080tgtgttcttg cttggaacct ttatctacgt ctacaaagtc ttcggcgtac cgggcggata 37140ctcagaatcg gccctcagtc gcattgtgag agaggcaaag caggcggagg taaaagaggt 37200cttcatagag aagaactttg gtcatggtgc gtttgaggcg gtaattaagc catacttcga 37260acgcgaatgg cctgccgagt tgaaagagga ttacgccact ggtcagaaag aggcccgcat 37320cattgagaca cttgagcctc ttatgtccgc acaccgcatc atctttaacg ctgagatgat 37380caagcaggac atcgatagcg ttcagcacta ccctcttgag gttcgcatga gctacagtct 37440atttgctcag atgtcgaaca tcacccttga gaaaggatgc ctgcggcacg atgaccgctt 37500agacgcgctg tatggcgcta tacggcaact gacctctcag atagactatg acgaggccaa 37560ccggataaat cgtctcaggg cgaaggagat gcgcgaatat ctggagatga tgaccgaccc 37620tctacgtcgc cgggagttct tcactggaca agaccacggg tatcgcaaat caactaacgt 37680gtccaatgcg atgcagtcta gggtgtttgg tggtagccgt gttaaagtga aatccagaaa 37740taccatttct tcaagaattt caaggacttg gtaattaggg gacactatag aaggaggccg 37800aggaataaca ggaagttata ggaggtcata ggtattccta ggtagtatag gtacgcctta 37860gtgggaggta tcctacctcc ctattccttc ctttatatta actatagata aggagtaata 37920atgcctaatc gtcctaataa ttatggtaat atgggtctga caggtaaacc tcgtcgtaaa 37980caagagaagc ctattgccac tgcactgatg gttccttttg cagaagatga agcccatgag 38040catggtgaga acatcgaagt acgtgagaac cgcattaatg accagaccaa atcaggtaag 38100cgccgtggtg ctatgctgct gacagacaag catggccttg tggttgcatc tggcagccgc 38160ttcaatgaca tctggtatag ctttaaattc gaagaaattg gtacaattca acctgcataa 38220gaaggagata acatatggca actatcaaat acggtgatgc tggtactgca actggtaagg 38280ctttcctgaa acagcaactg gaaaccacag cgactgcact gccacttcca atcgtgtcca 38340agtcagactt gggtcgtgca ctggcaccta tcaatcaggc tcgcctgtct ggtaagcaga 38400agggtgctat ggtaatcatg gaagatgacg gtacgcatga actgcacatt gcggtggctg 38460atggcccgct tccgactgac gcatggaaca tttgcagcct tgacggtgaa gtaactccgg 38520cacagggccg ctaaggaggc tagatgctac gacatcagat taacgggaat cacaacccgt 38580tacatgtaac aggccaacgc tcacggagta ataagagtat tgccatccag gagggtgtgc 38640ctattgtacg tgcttctgtt ctagcatctc cgacatctta catcaatgac cctcacctgt 38700caggtaagcg tgaaggtatg atggtggctg tactggcacc tgaagatgga gacaaggcag 38760gtctatatct ctacaggtgg gccagataaa cataacatca ttgaccatgc ggtgttcaaa 38820cattttgtaa ctaacggctt ggttgtaggc gctattgaga cgcacactgc caccactaac 38880aacatccatg tacgtatgca catcacagaa ggttctacgg gtgcatacac ctttagcttt 38940tcctttgagt ggacatctga cttcgactta ctggagtgat aatgttgaac aaatacttca 39000agcgtaacga gttcgcttgc cgttgtgggt gcggtacatc cactgttgac gcggaactgt 39060tgcaggttgt cacagatgtc cgtgaatact tcgggttacc tgtagttatt acatcgggtc 39120atcggtgcag tgaccataac cgccgcgtag gtggtgctgc atcttccatg cacatgactg 39180gcaaggctgc tgatattaaa gtgaaaggga aggacgcgag tgctatcgca tcctacttgg 39240aacacaagta ccctgataaa tatggtatcg gtcgatacaa ctccttcact cacattgacg 39300tgcgtgatgg taaggctcgc tggcgtggat aactgcattg catggtgtga gaagatggtt 39360gctaaggcat ctgctgaagg taactatgtt gactggcaga attacaccaa tctgcttaac 39420gaatggaaat ggagagcatt acgatgaaga aactattcaa gagcaagaag gtgatcggcg 39480cactagttac actgatcgtt gcgcttgtat cggtatggct tggtgttgac ctaggctcag 39540gtgcggagtc ttctgttacc gatgtggtct gccaagtaat tacctgtgag taggttactt 39600gaagtagtgg caggacttct tggcctgctg cttgcctata agaagaagca agaccagaag 39660gaggcgcaac atgaagcaga tctggctagc gatgaccctg ctgattggtt cgctgaccat 39720ttccgggtgc gggacggcgt taccagaaac tcagaaggtt cgtccaacca aaccgactct 39780gacggcagtt tacgagagag atgatagggt ctgcttcagt aagccagatg ctacacaatt 39840aggcttgtac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat 39900acacatacga tttaggtgac actatagaat agaagtatag tgccgttctt ttgagcggcc 39960tattactcac cagtcttcac ggggagggct ggatagtaat aggaggttta atgtcattaa 40020ctaaaccacg ttgcttcagg aaggcaagtt atctaagcca gttaggcact ttgcagaatc 40080tggctaacac tggagatgac gtacttgtta tcgatgttga ctacaagttc accaatggag 40140agactgtaga cttcaaaggt cgattggttc gtatagaatg cgaagctaga ttcataggcg 40200atggagcttt aattttcact aatatggcta gtggttctgt agtagaaaag cctttcatgg 40260agagcaagtc cacaccttgg gttatctacc cttggacaga agatggcaag tggattacag 40320atgcacaagc tgttgctgct acgcttaaac aatctaagac cgaaggatat caacctggag 40380tcaatgattg ggtcaagttc ccaggacttg aagcattgat accgcaagag gtgaaagacc 40440agtatgtagt atcaacactg gacatccgtg attgtgtagg tgttgaggtt agacgtgctg 40500gtgggcttat ggcagcttac ttgttccgca actgtcatca ttgtaaggta attgattctg 40560acaccatcat tggtggtaaa gacggcatca taacctttga aaacttaggt ggtgaatggg 40620gtatcggcaa ctatgccata ggtggtcgtg tacattatgg ctcatgtagt ggtgtgcagt 40680ttcttcggaa caatggaggt gcatcacata atggtggagt tattggtgtg acctcatggc 40740gcgcaggtga gtctgggttt aaaacatggc aaggttctgt aggtgcaggt acatctcgta 40800actataacct tcagttccgt gactcagttg cattatctcc agtatgggac ggctttgact 40860taggctcaga ccctggaatg gcaccagaag aggatagacc gggagattta cctgtatctc 40920aataccccat gcaccagtta cctaataacc acatggttga taacatactt gttatgaact 40980cattaggtgt aggtttaggt atggacggta gaggtggtta tgtgtcgaat gttaccgtgc 41040aggattgtgc aggcgcaggt atacttgctc atgcattcaa ccgtaccttc tctaacatta 41100cggtgattga ctgcaactac atgaacttcg attcagacca gataatcatc attggtgact 41160gcatcgtgaa tggcatccga gcagcgggta ttaagcctca gccatccaaa ggcatgatca 41220tcagtgcacc tcactcaacc ttgagcggta ttgtgggtaa tgtgccgcca gaccgtattc 41280ttgcaggtaa catccttgac cctgtgttgg gtcatacaag gattaatggg tttaatagtg 41340actcggcgga actgagcttc agaatccaca agcttaccaa gaccttggat agtggtgcta 41400ttcgctctac gctgaacggt gggccgggta ctggttctgc atggactgag atgactgcaa 41460tttcagggtc agctccaaat gctgtctcgt tgaagattaa ccgaggagac ttcaaggcaa 41520ctgagatacc agtagcacct actgtgcttc cagatgaagc ggtaagagac cacagctcta 41580tcgcacttta ttttgatcag gaagctcttt gggctttagt taagaagccg aacggaagcc 41640tcacacgaat gaagcttgct taatgtaggc agcgcgttag cgctgctttc acgcgaactt 41700ttcttaaagg ttatcatagt ggtagccttt cagaaaagga ggtgacatga tacaaagatt 41760aggttcttcc ttagtgaaga tgccaaatgg tattacattg acacagtggt tgcaacctgc 41820aaacatcatc aaggtagatg atgcaccata caatggagac cttattgctg catataatgc 41880tattcccgtt ataggtaatt atgctttggt tcttaccaac cacacttaca atgcagttgg 41940tttgtttgat gcaggtcgta acatgaagcc taacatcacc atcattggtg ctggtatgcc 42000tcaacttgca gatgataggt cgtcctttgt tgaaggttct ggcactatca ttaaaggcgc 42060agtcaagaac tccgccaagg gcttccagat tggtaaccta ggtattgatt gtggtaacac 42120agttagtcgt acagactacc aacctgcacg cttcgaagac ccactacaga tatacgggtg 42180tggcgctaat gctaacatct ttatcgataa cgtgaagtgc cttagtgcag tttctgtaga 42240cgagagaccg ggaacacaca gcattctgct tgagcaaact gaaggtgtta ctctcggata 42300tgtagagtgc attggtggct tccacggact taccatcaag tgccgtaacc tacgtggcgg 42360gattgcacat tgctatggcc agtatggtga tggcttcatc atcaaatctg acgctggtgg 42420tgcagcgagt catatctaca tggagcggat tcaagtgggg catccagatc aatctatgtg 42480gcctgatgta cacttaggtg gtatctacga tgctcatgat ggagtgacaa ttgacagtgt 42540tagtattggc gagttgcatg ttgtacgagg gtcttggggc ctgatacctg cggataacgc 42600cacgggtagt atcaccaact tccatattgg acattatgag tgtcacctta cttatggcaa 42660ctactactcc cttgttatca acgacaaggt tgtaggttgg actatgggta ctcacaacat 42720cacgacctgc tcaggtggca tcaaggtaga ccctgcatcg gtgtatgtaa acatcggaac 42780tggacgctcc accaacaaca ctgagagtgg gtactctctt ggtggacaca ccctgattca 42840tggtgaactg attgcagatg ctaatggtaa gtacggtgta gagtatacag gtggcctagg 42900tcttgatgta agtaagattc atgggttcca gaaccatctt ggtacttact caggctactc 42960ttctgctatc caatctctac tgtggcctga cgctgggttt gaagcgatgg ttacagggcg 43020cactgtgaca ttgcgtgggt ctctcacgaa aggtacgact gcatggtgtg gtcaggtact 43080cgatgctgtt aagcctacac gagacattcg tatatacgca tgggctgttg gtcttggtgg 43140ttctatggtt ccagtggaag catggattcg ttctgctaat ggagctatag acgtagtagg 43200aaaggactcg gtgggcgaag ggcagattgt tagcttcact ggcagctaca tattcaagtg 43260aggtctgtat gccattagtg aagtctatca aggagaaggc tgtacgccag aacacagaag 43320aactcatcaa gtcaggtcgt gaccctaagc aggcttatgc aattgctaag gatgtacaac 43380gtcgtgccat gaagaaacct tctgcatctt agtgtaacca aagggttggc ttaggttgac 43440ccttagtgta atcaaaggag ataacatgta tattccaatg gaagcagtag taggtatcgc 43500ttgtttgcta gtagggtttg tcataggttt gatagcacaa taatggtggt cacaaagtag 43560ccaaagtcaa aattttgata taggcgtgtg tcagctctct cggcctcggc ctcgccggga 43620tgtccccata gggtgcctgt gggcgctagg gcggcctgtg gaggcctgag agaagctctt 43680agtgtgggcc aaagggtaac ctgaggcctg ccggagcgag cgatagggac gcgtgtaggc 43740cgcttgacag cgtgtgtggg cgtgggcta 43769344385DNAArtificial SequenceSynthetic Polynucleotide 3tcgccctcgc cctcgccggg ttgtccccat agggtggcct gagggaatcc gtcttcgacg 60ggcagggctg atgtactcct tgtctagtac aagggaggcg gagggaacgc ctagggaggc 120ctaggaatgg cttagtggtg gacaaggtga ttaccttagt gaagcctctt agtgcattcc 180tgaggccatt cagggcgttt atgagggatt gacagggtgt gagggcgtgg gctatctgtt 240cctttgctcc tcacttcgtt cgtcgctgcg gtagcctgat gtgtacctta ggttattcct 300tgatggatag cttaggttag ccttagtgga ttaccttagt taaagcctta gtgcttcact 360tagtatcagc ttagtagtgt accttagtaa gtcttagtgt cttctcttag tgattgcaca 420tgcaagcatg taagatgcta ataggtcgcg gtcggcagac cgctaaagaa agagaatggt 480aataagatgc agtaggagga acaccagaag cctagccaac ctaagctatc ctagctctat 540atctattgct tttccttagt ctaacacgtt agacaaccta tcttattctt agtgatggta 600acttagtgtt gacaagataa tcttagtgta atactatgca tcacgtaggc ggtgctgagg 660cacctagtag ccagctagta aggcatacga agagactagc gcttacattg ctctttaaca 720atttgcttag tgtaacctat gtatgccgtg gttaactact tattgaatga ggtattaact 780atgacattaa ataaccgtga actgtccgtt ctcttcactc tgttgtgcta catgattcgt 840aacaacgaat tacttacaga tgatgagtta gccttgtatc accgctttct taacgaaggt 900tggaccgata cagttaatca ataccgtaac atgatagatg agttgaggga gggtaaataa 960tgtatcaaca tgaggtattc tttgaatcag ctagcgaagc tattcgcttc cgtgatgata 1020tgatgcaagc tggtgtaggc gttgatgtgt atcactattt gatagattac gacactgaat 1080atcaccgagt taccttagta tctgagtatg acaaccaagt cattactgag tatctaggca 1140gtgaagatta cgattacgat gaagtaatca cgacaaatct ctaaattaac tgttgacagc 1200cacggcatac aaggttacat taagcatcaa gacggcgacg tctttaaaca tcccgctctt 1260taacaatacg gtttgtgtct tgataggcta actaactaac taaggtaatt atcatgaaag 1320ggttaatttg tgtagaacgt atggtcaatg gtaaacttga aatattacca ctggaaaacc 1380aatctagctt caaagagtgg tatggctgtt tctcactgat ttaaggtaaa ggctggcact 1440agtcagccta tcaaggcgca aaccaagctc tttaacaatt tggatggtag cttcttagtc 1500tggataggtt aaacctagga gattctcttg agtctcctat aatgtaacct aactaactaa 1560atgaggatta aatcatggaa cgcaatgcta acgcttacta caaccttctg gctgcaactg 1620ttgaagcatt caacgagcgt attcagtttg atgagattcg cgaaggtgat gattactctg 1680atgcactaca tgaggttgta gacagcaatg ttccagttta ttacagcgaa atctttacag 1740tgatggctgc tgatggtatt gatgttgatt ttgaggatgc tggtttgatt cctgacacga 1800aggatgtaac caagattcta caagctcgca tctatgaagc tctttataat gatgtaccaa 1860atgacagcga tgtagtttgg tgtgaaggcg aagaagagga agaataagga tggaaaagca 1920atataacttt atcttttcag acggtgtaac cctgaagtgt tccctacgat tcgcacaaat 1980tcgtgaggaa gtactaggca ctacatacaa actatttagc tgacactata agagaaggct 2040taacaaggcg ttactaaggt agcgcctgat taaactttca cttactagga gttgagatta 2100tgaaaacctt gattggatgc ttcttgttgg cttctcttgc tctggcattt accgctaaag 2160ctggttatga cgcttataaa gtagaacaag cccagcaaga ctgggccaaa aaaaagttca 2220acttgtgcag caagagcaac acctacgagt actgcaacaa aacactaaga cacttatgga 2280aagagtaact agcctatagc ccacctgagt gggctatgtg atatttactt aacactatat 2340aaggtgatta ctatgactac tgaaaacacc ctcgtgtctg tccgtgaagc tgcaaccgct 2400gaaatcaagc aacatttaga caatatcggc acttcttaca tcaaagtagg ggcttgtctg 2460aatgagttac gcggagactt tgaaggtcaa aaagagtttt tagcctatgt tgaagcagag 2520tttgccatta agaaggcaca atgttacaag ctgatgagtg tagcccgtgt ctttgaaggc 2580gatgatcgct ttaaaggcgt ggcgatgcgt gtaatgctgg cgcttgttcc tttcgctgat 2640gaaaatataa tcatggagaa ggccgcagaa ctcgccgcaa atggcaagct ggacactaat 2700gccgtaaacg ccctgattga acctaagaaa gagtcaaagg ccgaaacggt acaatctaag 2760gctgagacag taaaaccgca ggagaacgcg actgagtccg cagaatcaca tgaaatgcaa 2820gcgccgcagg tagtgccacc cgcgagcgag caggagtccg acgaatcagc accttgggaa 2880gaggaaagca aaccggaagc gccaaaggca gctccgatgg ataacacggc taatactgag 2940aatgccgcta ttgctggtct gctggcacaa attaaagcac tgactgagca attacaggca 3000gccaatgacc gcatcgcctc cttaagtagc gcacgcgaaa gcaagaaggc atccgcacct 3060atgctgccgc agttcaaatc ttcctgcttc tacgctcgct taggcttgag cgcggaggag 3120gcaacgaaga aaacagcagt taacaaggca cgccgcgaac tggttaagct gggatacggt 3180gaaggccatg aggcatggcc cttaatctct gaggcagtag aagagttgac taagtaacct 3240tatcggtggc atcttcttag gtgtcaccta ttaaggtttc tttcactagg agtaaacaag 3300atgcaaggcc tacacgctat tcaacttcaa cttgaagaag aaatgtttaa cggcggtatc 3360cgtcgctttg aagcggacca acaacgccag attgcatccg gtaatgaatc agacacggca 3420tggaatcgcc gcttattgtc cgagttaatc gcgccaatgg ctgaaggtat tcaggcatac 3480aaggaagagt atgaaggtaa aagaggccgt gcaccgcgtg cattagcttt cattaactgc 3540gtagaaaacg aagtggcagc atatatcacg atgaaaatcg ttatggatat gctgaacacg 3600gatgtaacct tgcaggctat agccatgaat gtagctgacc gcattgagga ccaagtacgt 3660tttagcaagc tggaaggtca cgccgccaaa tactttgaaa aagttaagaa gtcacttaag 3720gcaagtaaga ctaaatcata tcgccatgcg cacaacgtag cggtagtggc tgagaagtca 3780gtagctgacc gtgacgctga tttctcccgc tgggaggcat ggcctaaaga caccttgctg 3840caaattggga tgaccttgct tgaaatctta gagaatagcg tattcttcaa cgggcaacct 3900gtcttcctcc gcaccttgcg cactaatggc ggcaaacatg gtgtttacta cctacagact 3960agtgaacacg taggtgagtg gataactgca ttcaaagagc acgtagcgca actgagtcct 4020gcctatgctc cttgcgtcat ccctccgcgt ccgtgggtat caccttttaa cggcggtttc 4080cacactgaga aagtagcaag ccgtattcgt ctggtaaaag gaaaccgcga acacgtccgc 4140aagctgacca aaaagcaaat gccagaggtt tacaaggctg ttaacgcgtt gcaggcgact 4200aaatggcagg ttaacaagga agttttacag gttgtggaag acgtcatccg tctagaccta 4260ggttatggtg taccttcctt taaaccactc attgaccgcg agaacaagcc agctaatcca 4320gtgccgctag aatttcagca cctacggggc cgtgaactga aagaaatgct tacgccggaa 4380caatggcaag cctttatcaa ctggaaaggt gaatgtacta agctgtacac cgctgaaact 4440aagcgcggaa gcaaatcggc ggcaaccgtt cgcatggttg gtcaggcccg taaatatagc 4500cagttcgacg caatctactt cgtgtatgca ctggacagcc gcagccgcgt ctacgcgcaa 4560tctagcacac tctcaccgca atcaaatgac ttgggcaagg ccttgctccg ttttaccgaa 4620gggcagcgtc ttgatagcgc tgaggcgctt aagtggtttt tggtgaacgg ggctaataac 4680tggggttggg ataagaaaac ttttgacgtg cgcaccgcta acgtgctgga tagtgaattt 4740caagacatgt gccgcgacat tgcagcggat ccgctgacct tcactcaatg ggtaaatgcc 4800gactcccctt acggcttcct tgcatggtgc tttgaatatg cgcgttatct ggatgcactg 4860gatgaaggca cgcaagacca attcatgacg cacctcccag tccatcaaga tggtagttgt 4920tctggtatcc agcactacag tgctatgcta cgcgatgcag taggtgcgaa agcagtaaac 4980cttaagccct ctgactctcc tcaagatatt tatggtgccg ttgcgcaggt agtaattcag 5040aagaattatg catacatgaa tgcagaggat gcggaaacct tcacttctgg cagcgtgact 5100ttaacaggtg cggagctgcg tagtatggct agtgcgtggg atatgatagg aatcactcgc 5160ggcctgacca aaaagcccgt aatgacacta ccttatggca gcacacgtct aacctgccgt 5220gagtcagtga ttgattatat cgttgattta gaagaaaaag aggcccaacg ggctattgcg 5280gaagggcgta ccgccaatcc tgtacaccct tttgataatg accgtaaaga cagcctgaca 5340cctagcgcag cttataacta tatgacagct ttaatctggc cttctatttc ggaagtggtt 5400aaagccccta tagtggcaat gaaaatgatt cgtcagcttg cccgtttcgc agctaaaagg 5460aatgaaggct tagagtatac cctgcctact ggcttcatct tgcaacaaaa gattatggct 5520actgatatgc tccgcgtatc tacttgcttg atgggagaaa tcaagatgag tctacagatt 5580gaaacagacg tagtggatga aacggcaatg atgggcgctg ctgctcctaa ctttgtgcat 5640ggtcatgatg ccagccacct tatcttaaca gtctgcgacc ttgttgataa agggattaca 5700tctatcgcag ttattcatga ctcttttggc actcatgcag gccgtacagc cgaccttcgt 5760gatagcttaa gggcagaaat ggtgaagatg tatcaaggcc gtaatgcact gcaaagcctg 5820ctagatgagc acgaagaacg ctggttagtt gataccggaa tacaagtacc agagcaaggg 5880gagtttgacc ttaacgaaat cttagtttca gactattgct tcgcataata ttaataggcc 5940attccttcgg gagtggcctt tcttttacct actacctgta acatttcatt aacataaaag 6000tgtctcacat gtgagactta tttaccggac actataggat agccgtcgga gacgggaaag 6060aaagggaaga taaaggatat aaaggaagta ataggtatta aaggttatat aggttatcta 6120ggaataccta ttaccttctt ccttcctctt attaccactc agaggaaggg cagacctagg 6180ttgtctcaca tgtgagactt cgtatttacc ggacagtata gataagatta actcactttg 6240gagatttaac catgcgcaac tttgagaaga tggcccgtaa agctaaccgt tttgacatgg 6300aagaggggca gaagaaaggc aagaagctga ataagcctgt ccgtgaccgt gcatctaaac 6360gcgctgcgtg ggagttctaa gttatggcta ttattcagaa tgtaccgtgt cctgcctgtc 6420aaaagaatgg

acatgatatt actggcaacc atctcatgat atttgatgat ggtgccggct 6480actgtaatcg tggacacttt catgataatg gtagacctta ctatcacaag ccggaaggtg 6540gcatcgagat aaccgagtta tctattactg gcaatatcaa atatacacct tctcaattca 6600aagaaatgga gaaggaaggg aagataagcg accctaaatt acgtgccatc gcacttggtg 6660gtatgcgtat gaaagaccgt tgggaggtca tgaatgaaca agaaagggca gagcaagaag 6720cagagtggaa acttgatgtt gaatggttcc tcacgcttaa gcgtaagaac cttgtttcca 6780ggcacattcg cggcgacatt tgcgcattgt atgatgtacg tgttgggcac gatgaagagg 6840gtagagtctc acggcattac tatccgcgct tcgaaaaagg tgagctagta ggcgctaagt 6900gtcgcacatt acctaaagat tttaagtttg gtcatttagg taaactcttt ggtatgcaag 6960atcttttcgg tatgaatact ttgtctcacg tgttagacaa gggaagacga aaggattgct 7020tgctcattgt cggcggcgaa ctggatgcac tagcagcgca gcagatgctc cttgattctg 7080ccaagggtac taagtgggaa ggccagccat accatgtatg gtctgtcaac aaaggcgagt 7140cttgccttga agagatagtg cagaaccgtg agcatatcgc ccaattcaag aagattatat 7200ggggttttga tggagatgag gtagggcaga agcagaatca gcaagcggct cgcctgtttc 7260ctggtaaatc ctatatcctt gaatacccct ctggttgcaa agatgctaac aaggcattga 7320tggctggcaa ggctaaagaa tttgtagatg catggtttaa tgccaagtca tctgatgaag 7380tctttggtag ccagattaaa tctatcgcat ctcaaaggga taagctcaag gctgcacgtc 7440cagagcaagg actgtcatgg ccttggccta agctgaacaa ggtaacgcta ggtattcgta 7500agaaccagct tatcattgta ggtgcagggt ctggtgtagg taagactgag ttccttcgtg 7560aagtagttaa gcacctcatt gaagaacacg gtgaatctgt aggcatcatt tctacagaag 7620acccgatggt caaggtgtcc cgtgctttta tcggcaagtg gattgataag cgtattgagt 7680tacctccaac caacgacccg aaagaagacg gataccgtga ggtgttcgac tataccgagg 7740aagaagctaa cgccgccatt gattatgtag ctgatacagg taagctgttt gtagctgacc 7800tagagggtga ctattcgatg gaaaaggtag agcaaacttg cctagagttt gaggctatgg 7860gtatttctaa tatcatcatt gataacttaa cggggattaa attagatgag cgtgcttttg 7920gtgggaaggt tggtgcactt gatgaatgcg tcaagcggat tggtactatc aaagaccgac 7980acccggttac tatattcctt gtatcacacc ttacacgtcc tccggcaaac cgtacccaac 8040acgaagaagg tggcgaagtt atcctttctg acttccgagg ctcaggcgct atcggattct 8100gggcatctta cgccttgggg attgagcgta atacaagagc tgaaacgctt gacgaaagga 8160ctaccacgta catctcatgt gtcaaagacc gcgaccaagg tatctacact ggaaccaagg 8220tcatgcttaa gggtgacatt caaaccggac gtttaatgga accacaagcc cgtactaagt 8280catttgatac aggtgaagca aggcaacaag aagtaccaga tttaccggat actatagaag 8340agactacctt cgatgaagaa agtgagttct gattagtgta tttatcaggc ttgtctcaca 8400tgtgagacag gctcttatta agtacattaa ataactggag attgattatg tataacttag 8460tgttgaatgt aggtgacttt gtacgcaaca tcaagaaaga ttcaagtcgc tatctttgcc 8520gtggtgttgt aacctttgta ggtgagaacc tgtattatgt agaatatcgc agtggtgtta 8580agcaatatta ccacaagaag acagcacata aatatcttga aaagattgta gagataaaca 8640atcaatgtaa gtgcatacat gatgaggttt gcgataaatg tgctcgccag atgcttaaga 8700atttcctagc tcctctttat tatggtgctg gtcctcaaac actagcagag tgcatggcag 8760aaaagaaaac cacactcaag aaagagcgtc gcaatgtaat cactggtaag actcaaagtg 8820agatgattaa gcaatgtggc actgcattag gtgttacaca gtttaatact cgtgcattgg 8880gtaaatccac aggacaagct atggtaaaga ttggagaagc catgatgcat ccaaatgtac 8940ctgtgcgaat catggatgtt gaccatgcaa tcacagaaca aggtacgcaa cgacgtgtaa 9000ttaataagca ttttgccgac actatagaag gcattattcg taagcaaggg ttgaaaggtc 9060ttcacatctt aaatggtgaa gaattactgt acctacctat cgttactgaa gaaacatacg 9120tgaatatcta aggagttaat catgactaag gtattaattt atatgcgtgg acctcataaa 9180tgctatgcag ttgtagcacc aaatggtgtt aagccttatc gtacttcaaa aagattggca 9240ttaataggtg ctagtagtag tgcaagtttc caaatggaac tttttggtca ttggactgaa 9300aggcaattcc gtgaggattt taaagtcatt ggcagcttca tggtgaaata tgcagaataa 9360acatagtctt agaatgttcg atggtcatga aaacctgcaa gccaagatta ctaaccaagc 9420cttcctgttc gcacagttaa ctatggctga ggctaagaag aatagtctca ctcgtgaaca 9480ggttatcaag gaggccactt gggaaccaca ccaaggtaaa tatatgggcc acaaattaac 9540tgtaacacgc agtcgataag tcaagggttg tccaacgtgt tggacagcct ttcatcatat 9600tgattgggag gtattaaatg actaagttta ctatgcaaga cctcattaaa ttacgtgatg 9660aaatagaatc accggaagtt aatacagagt ttcactacat tgatccacga gataaacgag 9720agattcctga ttatcagatt gagacggagt taatgtatga agattattga ttggaagaag 9780gaagcagaag gccgtatcct agtgatggat gcggaggcta aaggcctgct gggtgctatc 9840cgctacggtc atcgtgaaga tgtacacatt atttgctgca tggacttgct caccactgag 9900gagttcctct tcttcgaccc atatgagatg cgtgaccctg aagcaaggga acacttgaaa 9960gagtgggaag gccatcaaga tgggaccttg gttgatggtg ttaacttcct aaagcactgt 10020gaagccatcg tctcacagaa cttcctaggc tatgacgggc ttctctttga gaaagccttc 10080cctgacatct ggaagggatt taactacacc gagaggcgcg gcaagggcag actacgtgct 10140gacttgtgtc cggtacgcgt catggatacg ctggtcatga gtcgcctgtt aaacccagat 10200agacgccttc ctccgcaagc atatgccaaa ggtatgggta acgttgcccc tcactcaatt 10260gaggcgcacg gcattcgtat aggccgttat aagccggaga acgaggattg gtctaaacta 10320actgaccaca tggtacatcg tgtacgcgag gacgtggcga taggccgtga cctattcctc 10380tggctattta acggagaatg gacggagcac aaacgccgtg gcgtgaataa acgcactggc 10440ctaggtattg agacagcctt ccacatggag tccattgtga cgctggagat gagccgtcag 10500gccgagcgtg gattccgtct ggatatagat aaagcattag cacgatgcga ggaattggac 10560gctaagattg atgagacagt cgcagcgttc cgtccgcaca tgcctatgcg tatcaagtct 10620aaacctttta aaccggaaga aaagaatgaa gtatgccaac gcgcaaatga gtatggagct 10680agcaacaata tacctactgt ccttgacccc tctcactttc ttcacgcaga gagacgagga 10740gatcgcaaga cagtatggag tgtcactact aagtctggtg attggtcggc tagcgtcaag 10800aaagactttc ctcaccttag aggaaaccgt aatgacacgc caagtgtcaa gtggattggc 10860gcttactcgc ctgttacttt cgaagagatt cccttgggta acagggatac agttaagcaa 10920gtgctctatg attatggatg gaaaggtgtt gaatttaacg ataccgagca agcgcatctc 10980gatgagcatg gcgtattacc caagccttgg agtgggaaga taaatgaaaa gtcccttact 11040ttatggcaag agagagccgc acgtgaaggt aaaacagtcc ctgattggtg cttgggtatc 11100gctgcatggt acatactcgt atcccgtcgt ggtcagatcc tcaaccgtgg tgacgttgaa 11160gccttcgacc agaagggggt gtggccttcg caagctggta tacgaaagtg tcgcggcctt 11220gtacctgtag catttaacaa ggagttagga atcaatgcgc agcaatacta cgaaaggtac 11280ggatgctggc ctacgtcaga caaggatgac ggagaatggc gtgtgccagc tattgctatt 11340agtattggaa cttctacgtt ccgtatgcgt catcgtaacg tggttaatat tcctgcccgt 11400ggcttgtatc ctttacgtga tttattcata gcagggaaag gcaagctaat ccttggttgt 11460gacggtgcag gtcttgaact gcgtgtcctg tctcacttca tgaatgaccc tgagtaccaa 11520gagattgtac tgcacggtga tattcatacg cataaccaga tgaaggctgg tcttcctaag 11580cgtgatatgg cgaagacatt tatatatgcc ttcctatatg ggtctggtat agctaacctt 11640gcagcagtat gtggtgttac tgaggaagaa atggaggaag ttgtggcaag atttgaggtt 11700gaactaccat ctcttgcacg tcttcgtgag aatgttatcg cacaaggtaa caagtttggc 11760tacctacaag cacctgatgg tcattggggt cgcatccgta tgtctggtgg tgaacttaaa 11820gaacacacta tgcttaacgt actactccag atgactggtt ctctgtgtat gaaatacgca 11880ttggtcagag cgtttgcagt gatgcgcaag gaaggtgtgg ccttagatag catgggaaac 11940ccttgcggta tagctaacgt gcacgatgaa atccagatgg aagtccctga agatgaggtc 12000ttgtatctca actacgactt gcctttcacc ttagaagggt tcgaaacaga gaaggctgct 12060gtgaaagcag tgttcgatgc agaggagaaa cgtgttcatg tggattctga aggacgtatg 12120tggtctgctg caaatctcgt tagtgttgat gctggtgtac ttcattgcca gcgtcgttat 12180caccgtgcag ggcatatcat tgccgacgca atgacctggg cgggtcagta cctgaagatg 12240cgttgtccga tggcaggtga gtataagatt ggtgcaagtt ggaaggaaac acactgatgg 12300acaggtttga tattgtttgc ctattctcta ccttctttct tatattcctt atgcttgctt 12360gctatggaag tatgcgatta gatatacctg atgaagagga gggttacgat tgatgcaggc 12420atcttttatt attcttggag tcatattatt tatggtagta ttctgggctt tctctggcat 12480tgacccagat tgtgatggta actacgactg agttatactc aaggtcactt acgagtggcc 12540tttatgaata acttattcct acttattttg tctaacatga tttactggac actatagaag 12600gaaagcatag gtaatctagg tttataaggt agtataggta attaagtaaa tataggagat 12660ataaatatgt ctatggtaac tactctggta ttcgtggctc aatactttcg tggtcttgct 12720aataagttca agtccaaggc tatcaaagct attgaggctc gcatcgaagc agtacaggca 12780gagcaagtta aagttgaaga acatcgtagt tctcaaatga ttgactgtca taaccgctac 12840tatgcatctc gtgatgaact aaatgcacgt caagtcaaag aggtagaaga tatgctggca 12900cgtcaccagc aagagcgtga cagcctgaaa gctgaatttg aagagaacaa ggcatcaatt 12960gctcttgtac atcaagctgc atctgacagt ctgaagaaag agattgttat gctggaaatc 13020gaactggata acctgaccaa ataagggggg gttatgatgg aagaagtaat tcaagctaaa 13080catgtaggta ttatctttcg cgatctagag cagcgtaaag ttgcaggtca tactcgtctg 13140gctaaagagg aagacaccgc aatcactact gtagaacaag cagatgccta tcgtggacca 13200gagttcactc aaggtgaaac ttgtcaccaa ttgagcctat caatttgtga cactatggct 13260attgtaaatg tgcaagaagt cgaagagggt gagtgtgtca gttacatcta ccctttagat 13320actattgcac gcattaaggt aatccataag taattactag acactataga acaataggtc 13380ggcttagttc ggcctatgat tgtaaagtgt tgttgatgtt gaaccattgt gcatcttgca 13440caacccgata ccgtataggg ctttctagtg agtacatgct tgtgctcagt acaaagctaa 13500ctgacaatag gagactaaat aaatggcacg tggtgatttt gattttggtg ctcaggttac 13560taaatctgaa ggtaaagtct ttaagaatcc agaagtaggt gatcatgaag cagtaatctc 13620tggcatcatt catgttggtt ccttccaaga catctttaag aaaggtaata ccactgaagt 13680taagaagcca gcaaactttg ttctggttaa gattgtcctg atgggtgacg atgacaagaa 13740cgaagatggt tctcgcatgg aacaatggat ggctgtgcct ctgaagtctg gtgataaggc 13800aacactgact aagttcctga atgcagttga ccctaaagag ttgctgggtg gcttcgatga 13860tttcattggt gaatgcctga ctgcaacgat ggtcggttct ggtgataaga atgacgatgg 13920ctcattcaag tatgttaact ggaagggatt tggtggtatg ccggacaagc tgaagaaact 13980ggtcattgct caggttgaag aggaaggtct gtctatgaca ggtcacatta ccttcgacaa 14040gctgaccaaa gaaatccttg atgacatccc agccaacttg gtgcgtcaat acttcctgaa 14100cgagacgcct cgtggtaaga acctgtctgt tgctggttct cacgtagaag caatcattaa 14160agctgctcgt gaagaagacc cagaatggaa gaaggctaag aagaaagacg aggaagatgc 14220taccccagct aatcgtaaat ctctggatac tggtgagtct gttccacagg aagtacctga 14280agcagaagat actcctgcac cggagatgga tgaggacgcg gaatattaag gagaaaggat 14340gaaagtacaa atcgtaaccc tgcactgcaa gaaaggaatt acaactcttg gcggcaacac 14400ttttcactcc ttctctgaag gggacacata tgccgacctg cactacatct ggcgcgacgg 14460acagcacgtg gtgaactaca gcgacccagc tacggggaaa cgccacggcg tatcgcttcc 14520ggcgcatgac attgctcagg tgaacacagt tttataaagt ctcacgtgtg agacaaatcg 14580gtgtccggta tttactggac actatagaag agaagaattt taatcggcga taatgccata 14640accaacaaaa ggagaattta atatgttcaa gattgaaact atcgtaaacc gtgttgttaa 14700aggtgctgct ctggtatccg ttgagtcttt cattatcgtc gatgaaactg atcaactggt 14760agctggtact aaggcttacg atacccgtga agaagctcag gctaagattg acagcatggg 14820taacttcgct gctggtctgg agttcgctcg tgcttgcttc cctgagcagg ctgacaaagc 14880tcagattggt aaggctaata tcgtagctga atatctggat tgggttgctg ctggtaaacc 14940agtgaaagaa gttaaggctg ctgaagaagc tgaagctcca gcagaagaag tagctgcacc 15000ggaaactccg gtaagtgaag aggaagaatt ttgataatag caggtgttgc ctctgttagt 15060cctagctgac tatcacgctc acctcatcta atgccctgtc tgccttagtg taggcagggt 15120cttttgcgta atagttattg gagaatgaat tatgccgact attgaatctc gaattgaact 15180ggacattagc tacaatgcaa tcaccagaca gtatattggg gttgcctatg attacaaaac 15240tggtgagaag ctagtggagg tgagacaatg ggatgactat tggttaagac agaacctcca 15300tgatgcggtg tcctccttcc tgaaggagtg gcctacatgc gaccaaactt cgacttcgga 15360gctacagtat cggaagacaa taacctgttg ctgtggccaa ctgaaggtaa tcgaatcgct 15420ttaatagatg ctgatatgtt accttacatc atagggtata caatcagtga tatgacttat 15480gtacgagcca caactcgtgt taagtcaggg caagtcccct caatcaaaga tacacctgag 15540tgtaagcaag cgtgtgaccg tgtgaactcc ttgcttaact cttgggtgta tgcagcagaa 15600tgtgatgcag ctaagttgtt catgacgaaa tcagaagcta acttccgtgt ccgcctagca 15660ttcaccaagc cttataaagg tcaacgtaag accgagaagc ctccattctt ctatgaattg 15720cgagagcatc tcttagaggt tcacggtgca atcttggcag atggagagga agcagatgac 15780ctcatgagta tcgcacaatg ggacagccac cgccgcttcc agcaagatac aggtaacgag 15840ttccctatcg gtagtccaga gcataaagca ttctctgata cttgcatcgt ttccttggat 15900aaggatttga tgattgttcc cggttggcat ctacagccgg gtcaagagaa gaaatgggta 15960gagcctatgg gttggcttga gctacgccgt aaggctaatg ggcaagtcaa agatctaaaa 16020ggtgctggcc tcatgttcca ctatgcacag atgattatcg gtgatgatat tgataactat 16080gctggcatac caggtcgtgg tgctaaatat gcctatgatc ttctcaaaga ttgtaagaca 16140gagaaagagt tgtacatggc agtgctgggt gcttacaagg ctaagttcgg gcatggacaa 16200gttaaaatta agaattaccg aggtggttat cgtatcggca aagcctttga cctaatgctt 16260gagtgtggtc gcttatctca catggcaaga ttcaagggtg atatatggcg agccgataag 16320aacccaatct tgtggggaga tgatgcggaa tggttagcaa attaaaatca tcggaggtgg 16380cagcttataa gaaggaattg ctagataagc aaggatggaa atgccctctg tgtggcggca 16440gtctcaaagc tgtcacacct gtaaaccgtg tacttgacca tgaccatgag acaggattct 16500gccgcgctgt tgtatgccga ggctgcaatg gtgcggaagg gaagattaag ggtgttatct 16560ctggttatgg taaggctggt aacaaccgtt acttccagct tcaatggtta gagcgactat 16620atgaatactg gaagttacat agtacgcctc agacagataa gttatatcac aaacatcaaa 16680cggaggcaga gaagcgcgag gctaagaacc gtaaggcacg ccttgcttat gcaagaaaga 16740aggaggttaa agttgggtaa gctgcgcagc ttgtacaaag actccgaggt acttgatgca 16800atcgagcaag ctaccgacga gaaaggtaat gttaactaca atgagatggc acgtgtatta 16860tcgtgtcata ctgtgggtaa gaagattacc cgccagttgg ctcgatactg gcatggtcaa 16920ttcaagaaga ccaagaagaa tggtgattac taccagaccc ttctgcaaga agataagcgt 16980atcaaagaag agcgtaagct caggactcct gaccgctacg aggatttggc tattgtgcca 17040ttgcctgact cgcctcatcg aagtgtactg gtgatccctg atactcatgc accttatgag 17100cacccagata ccctagagtt ccttgcagcc gtggcagcac gttaccgtcc agacacagtg 17160gtacacctag gagatgaggc agacaaacat gccctgtcat tccacgattc ggacccaaat 17220ctggatagtg ctggcatgga gttagagaag gctcgtatct tcatgcacaa attgcacaag 17280atgttccctg tgatgcgcct gtgtcactct aaccacggct ctatgcactt ccgtaaggca 17340agcgccaaag gcatccctgt gcaatacctg cgcacctatc gtgaagtctt cttcccgcag 17400ggaggtggcg accagtggga ttggcaacat acgcacgtcc ttgagttgcc gaatggtgaa 17460caagtggcat tcaagcatca acctgctggc tctgtcctag cagatgcagc gcatgagcgt 17520atgaaccttg tgtgtggtca cttgcacggt aagatgtctg tggagtacgc acgtaataca 17580catgaacagt attgggctgt gcaaggtggc tgcttaattg atgagtcatc ccgtgcattt 17640gcctatggtc gtgagtctaa atacaagcca gcattaggtt gtgtggtcat tctggagggt 17700gtgcctcaca ttgtcccgat gcaaaccaat agcgacaacc gttggattgg caagatttag 17760ttgacactat agaacaaagg gctaggtaag actttatcgg ctggcgtatc caaatgatat 17820tgcactagcc cttgattgta tagtgaatgg aggattcaat atgtcacact atgaatgtaa 17880gaagtgtcat aagcgttatg attactgtac ttgtggtcaa gagaaaacat cttttaaagt 17940tggagacaag gtatttcgta atgaaaaaga ttcgattcct tggaatcaat actgcaaaga 18000agctggtatt gaccctgata gccctgtaac catagatgat attgatggca ttaacttgtg 18060ctttcgtgag gtgaggggta caggttggga ttccaaaaaa ttcaaacttg catctgataa 18120gttagacaac aatatggtaa ttaagcctaa gcactacgag ttctttgatg gcgtagaggc 18180aatcactatc attgcccgca gtatgaccga gaagcaattc gctggctatt gcatgggtaa 18240tgctttgaag taccgtctac gtgcaggtaa gaagttcaac actgaagaag acctgaagaa 18300agcagattac tacaaagagt tattccagaa gcatcgtcac gaatgtattg atgaggatat 18360ttgatatgaa tatctttgag ttcctaggtc ttccagaaga ccaccgcaat cacccattca 18420tgctggtgaa gcatcgcggt gaagttcctg agaagaaatt aacttttcca tgttatgcac 18480aggtgaaacg agatggtatc ttttctgctg ttgttgttcg cactgatggt gtcgttggca 18540tttttggtcg cactggtaag aaattggcaa acactgaagg actcgaacaa gcctttgcta 18600cctttccggt tggcatttat cttggtgagc ttcagtctat ggccattgat atctaccttg 18660aggcaatctc tggggttgtg aaccccaatc gcactgagcc acttgatttc ataggccagc 18720agattaaaga caacctgtat atcgacttct tcgatatgtt aactattaag gcattccatg 18780atggattcac tgatgtttct tatctcaaac gttacgatgc tttacatcgt cgtatcggcg 18840ctcatcttag cgggtgcaac gctatccttc ctatcactcc ttgccataat gagcgagaag 18900ttgaagcgtt tgcgcaagag caaatagatg caggacgtga gggtgctgta ttcaaactgg 18960actgcgatta tgaagcagga cacaaaggtt atcgtcagac taaagaagtc cgtaaggtaa 19020cctatgacct tacttgtatt ggctttgaag aaggtaaagg caaatacaaa ggtaaggtag 19080ctaacctcat tttcaaatgg aaaggaggca agacaatcaa agctatgtta ggtaaggggt 19140ggactcatgc agatgcagag cagatgttcc acgacattaa acatggtgga cgattgaatg 19200tcattggtaa aatctttgaa gtcaaaggtc ttcaggattc aagcaagggc aacattcgtc 19260tgcccaaagc gggagaatta agacatgaca aagatgaacc agatttcttt tgatagcatg 19320aaggcaactc gtgcagttga ggtagcagaa gctatcttcg aaactttatc ctgtggcatg 19380gaagtgccat atactttact tgctgatgca gaagaacttg gtctttctgt agaagctatc 19440caagagaagg ttgacgaatt atatggtaca gacgaagaag aaaccgacga tttcatttga 19500aggaatggag atgcttgaga tgattctcaa gccttcttct cctaaggtga ctaagactca 19560tgaagagtta atcgttgatg aagttaagcg ttacatcatg gattgtgtca gagcacaact 19620ggtggtccaa tgatacgtcc agcctccttc ctagatattc ctgagattat aaaccttggg 19680aataaatatg tggaagagga agtcaaggtt gtagcccacc actcagcctc atggaatgca 19740gaacaaagtg ccataacctt tgtgcatctc ttaatagaga cccaccactc agcctcatgg 19800aatgcagaac aaagtgcaca taacctttgt gcatctctta gtagagaaga tttatcccta 19860tgggttgctg tagatgaagg gcagattgta gggttcctgt gggctggcta tcacgagttg 19920gccccttgga cacctgtaag agttgcctct gacattctct tttatattat accagagagg 19980cgaggaacac tacttggtat gcgtctcatc aaagccctaa agcaatgggc tagtgataat 20040gaatgctctg aggttcgcct gtctatcgcc tctggtatta atgaagaacg tgtcggacgt 20100atgtataagc gacttggctt tgaaccgttt ggcactgtgt ataacctgaa gttctaagga 20160gataacatgg gtgttgtaaa gaaagcattt aaggctatcg gtcttgctca agatgcacca 20220cgtattgaag ccaaagtccc agcacagcag cttgagcgta agcctgagac tgaagctgaa 20280gatattcaaa ttggtgcagg ggatgatgct actgcatctg caaaaggtaa gcgtggcctt 20340gtccgtccgg tagcttctag cttgaaggtg taatatgaaa cagagcatag atttggagta 20400tggaggtaag cggtctaaga tacctaagct atgggagaag ttctccaata aacgtagctc 20460tttccttgat agggcgaagc attactccaa attaaccttg ccctatctga tgaatgacaa 20520aggtgataac gagacttcgc agaatggatg gcaaggtgta ggtgctcagg caaccaacca 20580tctagccaac aagctagcgc aagtactatt ccctgcacag cgttccttct tccgtgtaga 20640cttaactgca caaggtgaga aggttcttaa tcagcgtggc ctgaagaaga cagagctagc 20700taccatcttc gctcaagtgg aaacacgggc aatgaaagag ttagagcaac gtcaattccg 20760gcctgctgta gtagaagcat ttaagcatct tattgttgct ggcagctgta tgctatacaa 20820gccgagcaaa ggtgcaatca gtgctatccc aatgcatcac tacgtagtta accgtgatac 20880caatggcgac ctgttagaca ttatcttgct acaagagaaa gccttacgta cctttgaccc 20940agctacacgt gcggtagtag aggttggcct gaaaggtaag aagtgcaagg aagatgacag 21000cgttaagctg tacacacatg ctaagtatct tggtgatgga ttttgggaac tcaagcaatc 21060tgctgatgat atccctgtgg gtaaggtgag taaaatcaaa tcagaaaagc tacctttcat 21120cccattaact tggaagcgaa gctatggtga ggattggggt cgacctcttg cagaggatta 21180ctccggtgat ttattcgtta tccaattctt atctgaagcg gttgcccgtg gtgctgcgct 21240gatggcagat atcaagtacc tgattcgtcc tggtgctcaa actgatgttg accactttgt 21300taactctggc actggtgagg ttgtcactgg tgtagaagaa gacatccata ttgtacagtt 21360aggtaagtac gcagacctca cacctattag cgcggttcta gaggtataca ctcgccgtat 21420cggtgttgtc ttcatgatgg agacaatgac acgccgtgac gccgaacgtg ttactgctgt 21480agaaatccag

cgagatgcgt tagagattga gcagaacatg ggtggtgtat actccctctt 21540tgctactact atgcaatcgc cagtagcgat gtggggtctg ctggaggcag gggagtcctt 21600cactagtgac ttagtggacc ctgtgattat cacaggtatt gaagctttag gacgcatggc 21660tgagttggat aaactggcta actttgctca gtatatgtca ctgccattac aatggcctga 21720gcctgtccta gctgctgtga aatggcctga ctatatggat tgggtgcgtg gtcaaatctc 21780tgctgaactg ccgttcctta aatcggctga agagatggca caagaacagg aagcacagat 21840gcaagcacag caagcacaga tgcttgaaga aggtgtggct aaggccgtgc cgggtgtaat 21900tcaacaagaa cttaaggagg cgtaatgtct ttctcattta ctgaaccgtc aaccactcac 21960cctactgctg aagagggtcc ggtagaaacc aaggaggtaa caactgatgc tgctactact 22020gatgctcctg ctgacgctgg cacttctgta caagatgaca atgctggtgc acaacctact 22080gaagacaccg gaggagaagc ttctggacag ccttcagaaa aaggagacaa tggcggagag 22140aatggtgaac ctaagccaga tgataccgcg accgacactg aggaagtgca atacttcttc 22200ggagaacatg aagtaacagt agacatccca caggatgtaa ctgacagcct taaagagaaa 22260ggcattgatg ccaagcaggt tgccaaggaa ctctattcca aaggtggcaa gtttgaactg 22320tcagatgcaa ccaagcagaa attgtatgat gcttttggca agtttgcggt agatgcttac 22380ctatcaggtc taaaggctca aaatgaagcc ttcttcctga aagaagccaa cgcagctaaa 22440gagttggaag cagctaacac ccaacgcttc tctgatgttt ctaaggaaat tggtggcgaa 22500gaaggttggt cccgtcttga ggagtgggca cttgaagcgc tgtctgatga cgaactaatg 22560gcattcaatg cggtgatgga atctggcaac cagtacctgc aacaatatgc tgttcgtgaa 22620ctggagggtc gtcgtaagca ggcacagggg gatgataagc catccctgat tgagccatca 22680gcacctgcta aggctaatga agagaatggc ccactgacgc gagatcagta cgttcaagca 22740atcgcaactc ttagccagaa gtacggcaat gaccgtaaag ctatggcaga agctcaggct 22800aaactggacg cccgtcgccg tgctggcatg gctcgcggta tctaattcag tatttactgg 22860acactataga agggagaaaa gttctcccta gttatcaatt tgatttataa ggagattata 22920atacatgtct acaccgaata ctctgactaa cgttgctgta tctgcgtccg gtgaggttga 22980cagccttctc attgagaagt ttaatggtaa ggtcaatgag cagtacctga aaggtgagaa 23040cattctgtcc tactttgatg tacaaactgt tactggcact aacacagtga gcaacaaata 23100tttgggcgaa actgagttgc aggtgctagc accgggtcag tcccctaatg ccacccctac 23160tcaggcggat aaaaaccagt tggtaattga taccactgtc attgctcgta acactgtggc 23220tcacatccac gatgtacaag gtgacatcga tagcctgaaa ccaaaactgg ctatgaacca 23280agccaagcaa ctgaaacgtc tggaagacca gatggcaatt cagcagatgc tgttaggcgg 23340tattgctaac accaaggccg aacgtaacaa gccgcgtgtt aaagggcatg gcttctctat 23400caacgttaac gtaactgaga gtgaagcact ggctaaccct cagtatgtta tggctgcggt 23460agagtatgct ctggagcaac agcttgagca ggaagtggac atctctgatg tagctatcat 23520gatgccgtgg aagttcttca atgctttgcg tgatgcagac cgaattgtag ataagactta 23580cactatcagc cagtctggtg caaccattaa tggcttcgtt ctctcttctt ataactgccc 23640tgtgatcccg tctaaccgat tccctacctt cgctcaggat caggctcacc acctgttgtc 23700taatgaagat aacggctatc gttatgaccc tatcgcagag atgaatggtg cagttgctgt 23760tctgttcact tccgacgcac tgctggtggg tcgtaccatt gaagtgactg gtgacatctt 23820ctatgagaag aaagagaaga cttattacat tgacaccttc atggctgagg gtgcaatccc 23880tgaccgttgg gaagcagtgt ctgtagttac cactaaacgt gatgcaacta ctggtgatgc 23940tggaggtcct ggtgatgatc acgcaaccgt actggctcgt gcacagcgta aggctgtata 24000tgtcaaaacc gaaggtgctg cggctgcatt ctctgctgcc ccagcaggta tccaagcgga 24060agaccttgta gcggcggtac gtgctgtaat ggcaaatgac attaagccga ctgcaatgaa 24120acctactgag taacacctat gccctatcta ccttgcgtag gtagggttct ttttgttagg 24180aggattcatg cctgtaatta gacaaaccag taaattagga catatgatgg aagatgtggc 24240cttccagatt attgatagta agctggaagc ggtaaacttg tgtatgcgag ctattggtcg 24300tgagggtgtg gattccctcg actcagggga cttggacgca gaagatgcaa gcaaaatgat 24360cgacatcgta tcccagcggt tccagtacaa caaaggaggt ggctggtggt tcaatcgtga 24420accaaactgg caacttgcac cagacactaa cggtgaagtt aatttaccta acaactgcct 24480agcagtattg cagtgttatg ctttaggtga aaagaaagta cctatgacta tgcgagcagg 24540taagctctac tctacttgga gtcacacctt tgatatgcgt aagcatgtta atgctaatgg 24600tatgattcgt cttaccttac tcaccttact accctacgag catctaccta caagtgtaat 24660gcaggctatt gcctatcaag ctgctgtaga gtttattgtg tctaaggatg cagatcagac 24720taagctagcc actgcgcagc agatagccac tcagcttctt atggatgtac aatctgagca 24780aatgtcacag aagcgattaa acatgctggt acataaccct actcagcgtc agtttggtat 24840catggctggt ggctctcaga atgtacctgc ttactctcat tcaccttatg agagttgggc 24900gctccgtccg tgggaggatc gttaatggaa gtacaaggtt cattaggtag acaaatccaa 24960gggattagcc agcagccgcc agcggtacgc ttggatggtc agtgcacagc tatggttaat 25020atgatacctg atgtagtgaa tggtactcaa tcacgcatgg gtacaactca tattgcaaag 25080atacttgatg cggggactga tgacatggct actcatcatt atcgcagagg tgatggtgat 25140gaagagtatt tcttcacgtt gaagaaagga caagttcctg agatatttga taagtatggg 25200cgcaaatgta atgtgacttc acaagatgca cctatgacct acctctctga ggttgttaat 25260ccaagggaag atgtgcaatt catgacgata gctgatgtta ctttcatgct taatcgtagg 25320aaagtagtta aagctagtag caggaagtca cctaaagttg gaaacaaagc cattgtgttt 25380tgtgcgtatg gtcaatatgg tacatcttat tccattgtaa ttaatggggc caacgctgct 25440agttttaaaa caccggatgg tggaagtgca gaccatgttg aacaaattcg aactgaacgt 25500atcacttctg aattgtactc taagttgcag caatggagcg gtgtgagtga ctatgaaata 25560caaagagacg gtactagtat atttatcgag agacgggatg gtgctagctt tacaataaca 25620accaccgatg gtgcaaaagg taaggactta gtggctatca agaataaagt tagctctact 25680gacctactcc cttctcgtgc gcctgctggt tataaagtac aagtgtggcc tactggcagc 25740aaacctgagt ctcgttactg gctgcaagct gagcctaaag agggaaacct tgtgtcttgg 25800aaagaaacaa tagctgctga tgtattactt gggtttgata aaggcacaat gccttacatt 25860attgaacgta cagatatcat caacggcata gctcaattca agataagaca aggtgattgg 25920gaagatcgta aagtagggga tgacttgact aaccctatgc cctcttttat tgatgaggaa 25980gtaccccaga caataggtgg aatgttcatg gtgcagaacc gcctatgctt tacagcaggt 26040gaagcggtta ttgcttctcg tacatcatac ttcttcgatt tctttcgtta tacggttatc 26100tctgcattgg caactgaccc ctttgatatt ttctcagatg ctagtgaagt ctaccagcta 26160aaacatgcag tgaccttaga tggcgctacc gtgttgttct ctgataagtc acaattcata 26220ctgccaggcg ataagccttt agagaagtca aatgcactgc ttaagcctgt tacaacattt 26280gaagtgaaca ataaagtgaa gccagtagta actggtgaat cggtaatgtt tgccactaat 26340gatggttctt actctggtgt acgagagttc tatacagact cttatagtga cactaagaag 26400gcacaagcaa tcacaagtca tgtgaataaa ctcatcgaag gtaacattac caacatggca 26460gcaagcacca atgtcaacag gttacttgtc actaccgata agtatcgtaa cataatctac 26520tgctacgatt ggttatggca aggaacagac cgtgtacaat cagcatggca tgtatggaag 26580tggcctatag gtacaaaggt gcgaggtatg ttttattctg gtgaattact ttacctgctc 26640cttgagcgag gagatggcgt gtatctggag aagatggaca tgggtgatgc actaacctac 26700ggtttgaatg accgcatcag aatggatagg caagcagagt tagtcttcaa gcatttcaaa 26760gcagaagatg aatgggtatc tgagccgctc ccttgggttc ctactaaccc agaactttta 26820gattgcatct taatcgaggg ttgggattca tatattggcg gctctttctt attcaagtac 26880aaccctagtg acaatacttt gtctacaacc tttgatatgt atgatgacag ccatgtaaaa 26940gcgaaggtta ttgttggtca gatttaccct caagagtttg aacctacgcc tgtggttatc 27000agagacaatc aagaccgtgt atcctacatt gatgtaccag ttgtaggatt ggttcacctt 27060aatcttgaca tgtaccccga tttctccgta gaagttaaga atgtgaagag tggtaaagta 27120cgtagagtat tagcgtcaaa ccgtataggt ggtgctctca ataatacagt aggctatgtt 27180gaaccgagag aaggtgtctt cagatttcca ctgagagcta agagcacgga tgttgtttat 27240cgtattattg tagagtcacc tcacacattc cagcttcgtg atattgagtg ggaagggagc 27300tacaatccaa ccaaaaggag ggtctaatgg ctataggttc agccgttatg gctggtatgt 27360cttctattgg tagcatgttt gcaggcagtg gtgcagcagc cgctgctgga ggtgctgccg 27420caggtggcgg aggtttgcta ggttcactag gtggattcct aagtggctct actgctggtt 27480tctctaatgc tggccttctt ggtgctggcc ttcaagggtt aggcttgatt ggtgatctat 27540ttggtggaag tgatgaagcc aaggcgatga agaaagcaca agaagagcaa tggcggcagc 27600agcttattgc tacacaagag gcgtacaaga cagtggcaga cgcagaacgt tctgctgcta 27660aacaatatca tgcagatgca atcagtaatc aggcttcact gctacagcag cgagcacagg 27720ttgcattact tgctggggct actggtactg gtggtaattc tgtgtcctct atgcttaatg 27780acttagcagc agatggcggc aggaaccaga gtactatcat tgataactat gagaatcaga 27840agattaattt caccaaccag cttaagtcta tccaacgtgg tggtcagatg cagatgcgtg 27900agtttaagaa gccttctgct atgaatacct tggttaaagg tattccaagt ctggcatctg 27960cctatgtaac tggtagtaag tctggcaagg cattgggtaa agccttaact gattctcgca 28020catattcatc tggaacaaga ggtatttaat ggcaattgag cgacaagcag tacaaggtct 28080gccacaagtg caggccactt ctcctaatgt catgaccttt gcacctcaac aagtgggagg 28140tgtggaggct ggcgtggctt ctacctccgg tagtaggttt atcgaagacc ttattcgtgc 28200agcaagcagc gtggctgatg ttaccactgg tatccttaat cagaagattg aggaagataa 28260ggttgttcaa atggaacggg catataacgg attaatgcct tctgaggatg caactcgtgg 28320tggcgctcgt gctaacatgc ttgtcaaagc tcaactgcta gctaatgatg aagcagcacg 28380aatgaaagac atggctactc gtttccaagg aacggatgac gaatggacac aacttatggt 28440tgactctcgt aatgagatgc agaataagct gttccagcaa taccctgagt tgcaaggtga 28500caaagatact atgcgtatgg tcactaatgt cttccaagaa cagcagcctc agatttgggc 28560tacacgaacc cagcataaac ttgaccgtga acaagcagac cgtgaggata cctttgacgg 28620gcgagtggct tctacttggg attctaatat tgaccctgaa gcctctggct atgctttaca 28680ggaacgaatc cgcgaaggtc ttactcaagg attactacct gaacagatgt acaagaagtt 28740agtccagcga gcaatttcac ttgcacaagg cggtgatgtt agcatggctg aagccctgaa 28800gtatgtgaag gacgataagg gtgtttctgt ttatgctaag aatccacagc ttatcacagc 28860catcactagt ggtaatgcag tttgggctag gaataatgta gctgatgtaa ctcgtatgtc 28920tttcgaagtt aaagaatcct accttgcagg tgatttaact gatgaagaat tgttggaacg 28980agcacagcac attaataatc tgacaggtaa ctctgtcttc tctaatccag aactagaggc 29040actgatgcgc caacgggcta agcagaatgc agagctaggt gcaatgcagg atatgcgacg 29100tgagctttac tccgaccgcc tgactggctt ccaaggtaag actgataaag agaagaaggc 29160ttacattgat gttatcaaac aggatagcca actttatgca gaccagcaaa tcaaacaacg 29220tggcttggac ccttacagtc aagaggctga agctattcgt ggtgcagtgg aagtgcagcg 29280cctgcaattc atgaactcca aaggcttagt ggatgatacc tttgagtctc gtatcaaagc 29340catggaatct atgctatcgc ctgagcactt tgccaagggc gaaccacagg agttgatgac 29400tattcgccag ttgtgggaac agttaccaga agagagccga ggtgtctttg gtgacacggt 29460gaatggctac atggataact acaacactgc actacaaatg ggagagacac ctttgcaggc 29520tgcaaggttt gcgcgtaaag cacagcagaa attctctcgt actgagaagg aaaccaagaa 29580gttcaactca gctattggag atgcactgga tgaggtatct ggtgctggct ggtttgatgg 29640taaaaccgaa gtgtcagact taggtaaagc tattgcggaa gaagagttac gagctaaggc 29700caatatgttg tggtctagtg gtatgcgtaa catggattcc atcaagaagg ctttaattac 29760ttggggcaat aaacgctaca ctcaatcaga ggatgcaaag acttccggtg gctatttcat 29820taaaggtgat tacacttctg catctgatat gcttatgtca gttgggaaag gcgtaaaccc 29880taccgatgta cctctggcgc ttggtaggta tgtagaaaca cagatgccag aattgaagaa 29940ggagcttcaa gagggggaaa ctaaagatga tatatacatt gattacaatg aacagaaagg 30000tactttcgtg attcgtgctg gtgcagcagg tcgccctctt tctggagtaa tccctgtaac 30060ctctttagat accacttcac tactagattc tgcctatcag aagaaagtag aggaacgaga 30120taaaggcgag tatgttcacc cgtatcgtac agatattggt gcacaagagc ctatgccagc 30180taaaccaact gccaaagata ttggtaaatt tggactagct aacttcctca tgtcttctgc 30240ttttgcttct ggtgagaatc tgccttctaa cttcgagatt aactatcgag gtaatatgca 30300acaattctat gacaagctag ctatggatga gaataaagat aaagttggct ttaataaggc 30360aactggaacc tttactccat ataaagacgc tcacggtgag tctatcggtt acggtcattt 30420cttaacggaa gaagagaagc gaaacgggta tattaagatt ggcgatgaac tagttcccta 30480tcgagggtct atgtctcagc ttacagagag caaggctcgc gctcttatgg agcaagatgc 30540taagaagcat gtgcctccta ctcgtgactg gaagattccg tttgaccaga tgcaccctgc 30600acagcaacgt ggcttgatgg atttaagcta caatttaggt aaaggtggaa tccagaactc 30660accgcgtgct cttgctgcat tcaaagctgg taagcttacg gagggcttta tcgaaatgct 30720gggcactgca tcaagtgaag gtaagcgtat tcctggccta ctgaagcgac gcgctgaggc 30780atacaatatg gcatctgctg gtggtgtgcc taagattacc gaagtggaga ctcgtgaaga 30840tggctccatg tgggttaggt ttggtggacc tatgccagca ggttctgtct cggcatggac 30900tcataaacgt attggcgcgg atggttggta tcaggtttat gaggctgcac ctaccaagtt 30960agctaaagat tctaaggtag gtaaagttaa gttgtagtac ctaactcaag gcttgtctca 31020catgtgagac aggtctttat gataggcact atggaggaat tatggaacaa gacattaaga 31080ctaattgggc tggatatgtc cagtctactc ctgagccgtt ttctattgag gcggctccgg 31140tatcggctcc tacgatacgc cagcgtaatg agttacaaga gcaagttctt gaagctaaag 31200ctgacgctga tatcttaggt gctgtaggtg ctgccttcca gaatgagtgg ttggcattcg 31260gaggcaagcg gtggtatgac cgtgccactg ctgatttcac acctcaacca gactttgaga 31320tacaacctga gcaacgtgaa gcactacgtt tcaaatatgg tacggatatg atgcagacaa 31380tcactgaggg tgttcgttct gaggatgaat tgaacttccg tattcagaat gcggatgaag 31440accttgagcg caataagcgc attgctcagg ctggctgggt tggctctgtg gcgacgattg 31500gcgctgctgt gcttgaccct gtgggatggg ttgcctctat tccaaccggt ggtgccgcta 31560aagttggact cgtaggccgt gctgtgcgtg gcgctatcgc cgctggcgtg agtaatgccg 31620ctattgaatc cgtattggtc caaggtgaca tgactcgtga tttagatgac attatggtag 31680cactgggttc cggtatggct atgggtggcg ttattggcgc tgtagcgcgt ggtagggcca 31740ctaagctcag tgagcaaggt gatgacaggg ctgctagcat tgtgcgcagt gcagacgcag 31800gggaccgcta tgttcgtgct gttgccgatg acagtatcgg tgcgatgcgt gttaagggcg 31860cagaggttct cactgagggt gtattcgata tctccagtaa gagtgaagac ctactgaaaa 31920ccttgcaacg agaaggtaat gcgattgata tgacacctcg ccgttgggct ggaactatgt 31980ctgccctcgg tactgtcgtg cactcatcta aagatgcaag tatccgaggc cttggtgctc 32040gtctgtttga atccccacaa ggtctaggta tgcagaaggc atctgctagt cttatgcaga 32100atactaactt aaatcgcctg aaatctgctg atatgaaccg cttcaatgat gggtttgatt 32160tgtggcttaa agagaataat atcaatccag tagcagggca taccaactct cattatgtac 32220agcaatacaa tgaaaaggtg tgggaggcag tgcgtattgg catggatgag tctacaccta 32280aatctatccg catggctgct gagggacaac aggctatgta cagagaggcg ctggctttac 32340gtcaacgttc tggtgaagcg ggatttgaaa aggtaaaagc cgacaacaaa tatatgcctg 32400atatctttga tagtatgaaa gccagacgtc aattcgatat gcacgataaa gaagacatca 32460tcgaactttt ctctcgtgcc taccagaatg gcgctcgtaa gattccaaag gaagcagcag 32520atgagattgc acgagcacag gtaaatcgcg ttgctgatgc taccttaact ggaaagctta 32580gttttgaaaa ggcaatgtca ggtcagacta aggcagagta tgaagctatc atgcgtaagg 32640caggcttcag tgatgaagaa attgaaaaga tgatagaagc tctggataac aaagaaacca 32700gagataacat ctctaaccga gctaaaatga gtttaggatt agatgttact caagaataca 32760atggcattcg tatgcgtgac ttcatgaata ccaacgtgga agagctaaca gataactata 32820tgaaggaagc agcaggtggc gctgcattgg ctcgccaagg cttctctacc tatcaggctg 32880cacttaatgc aattgacctt gtagagcgaa atgcacgaaa cgcggctaag gatagcaagg 32940ctagtttggc attagatgaa gagattcgtc agatgcgaga aggtcttcgc ctgattatgg 33000gcaagtcgat tgatgcagac ccacaggcta tatctactaa gatgatgcgt cgtggtcgtg 33060atatcacagg tgtgcttcgc ttaggtcaaa tgggcttcgc acagctaggt gaacttgcca 33120actttatggg tgaatttggt attgctgcaa ctactatggc tttaggtaag caattccgct 33180tcacctctaa ggcgttgcgt aatggcgatg gcttcttccg agataagaac ttagctgagg 33240ttgagagaat ggtggggtac attggtgagg ataactggct aacaactaag ggtgcacgtc 33300ctgatgaatt tggtgatgta accacagtaa gagggatgat ggctcacttt gaccaatcca 33360tgaactcaat acgtcgtgct caaaccaacc tatcactctt ccgcatggca cagggttctc 33420tggagcgaat gactaatagg caaatagctt tgtctttcat tgaccacctt gaaggcaaga 33480agattattcc tcagaagaaa ctggaggaac ttggtcttac tcaggagttc atgactaacc 33540tacagaagca ctatgatgct aactctaaag gttctggctt gcttggcttt gatacaatgc 33600cttatgccat gggtgaaact ttagctaatg ctattcgtcg taagtcaggt ctaatcatcc 33660aacgtaactt cattggtgat gaaggtatct ggatgaacaa agcactaggt aagacatttg 33720cacagcttaa gtcattctct cttgtatctg gtgagaagca atttggtcga gggattcgcc 33780acgataaaat tggtcttgct aagaagacag cttacgggtt tgctttgggt tcaatagtgt 33840atgcggcaaa agcctatgtg aactctattg ggcgagaaga ccaagatgaa tatttggaag 33900agaagttatc gcctaaaggg ttggcctttg gtgcaatggg tatgatgagt acaactgctg 33960tatttagtct aggtggagat ttcttaggtg gcctaggtgt tctaccttcc gaactcattc 34020aatcacgcta tgaagcaggt ttccaaagta agggtctgat tgaccaaata cctctggttg 34080gcgttggtgc agatgcagta aatctggcta actcaatcaa gaagtatgca gaaggtgaca 34140cagaaggtgt agatatcgct aagcgagcac tccgtcttgt gccacttacc aatataatag 34200gtgtccaaaa cgcattgcgt tatggcttag atgaactgga ggattgatga gttatacttt 34260cacagaacat acagccaatg gtacgcaagt cacctatcct tttagctttg ctggtaggga 34320taaaggttat cttcgtgcct cagatgtgat agtggagtct cttcaaggta acacttggat 34380tgaagttaca tctggctggc aactaactgg cacgcaccag attacttttg atgtagcacc 34440agttgcaggt ttgaagttcc gtattcgaag ggaagtacaa aaagaatatc catacgctga 34500gtttgaccgt ggtgttacct tggatatgaa gtctttaaat ggttctttca ttcatatact 34560ggagattaca caggagttac ttgacgggtt ttatccagaa ggatacttca ttaaacagaa 34620tgtaagctgg ggcggcaata agattactga tttggctgat ggcacaaatc cgggagatgc 34680agtaaataaa gggcagcttg atgccatcga caagaagcat acagattgga acgccaaaca 34740ggacattgag attgctggcc ttaaggctgg tatgacttct ggtattgcgc acagaactgt 34800tccttggtac acgatagccc aaggtggtga gatttccgta aaaccacctt atgaatttca 34860agatgcacta gttttcctta atggggtatt gcagcaccaa attgtaggcg catactctat 34920aagcaacaac actatcactt tcgcagagcc gcttgtggct ggtacagagg tgtatgtgct 34980gattggtagt cgtgtggcta catctgaacc taatattcag ttggagttga actttgactt 35040agtagaaggc caacaagtag tacagattgg ctctgcattt aagtacattg aggtctacct 35100tgatggatta ttacaaccta aacttgctta tcaggtagac ggtgacattg ttactttctc 35160agaaagagta ccagaatgcc ggatgactgc taagattatc acagcataag gaggtgggat 35220gattaactcc gaactggtag atagtggtgt gaagcttgcg ccacctgcac tcatatcagg 35280tgggtacttc ctcggtatca gttgggataa ttgggtgtta atagcaacat tcatttatac 35340cgtgttgcaa attggggact ggttttataa taagttcaag atttggaggg agaagcgtga 35400gcgtacacaa taaacatgca gctacagagg acgaggttgg cattctgcat ggtgctatta 35460ccaaaatctt caataagaaa gcacaggcaa tactggacac tatagaagaa gaccctgatg 35520cagcattaca tttagtgtct ggtaaggata ttggtgcgat gtgtaagtgg gttcttgata 35580acggcattac cgccacacct gctgcacagc aggaagagtc caagttatct aagcgcctca 35640aggctatccg agaggcatcc agtggtaaga taattcaatt cactaaggag gattgatggc 35700taaggcaaga gaatcacaag cggaggctct tgccagatgg gagatgctac aggagttaca 35760gcagaccttt ccttacaccg cggaaggttt gcttctcttt gcagatacag ttattcataa 35820cttaattgca ggcaaccctc atctgattcg tatgcaggcg gatatcttga agttcctatt 35880ttacggacac aagtaccgcc tcatcgaagc gcctcgtggt atcgctaaga caacactatc 35940agcaatctat acggtattcc gtattattca tgaaccgcat aagcgtatca tggttgtgtc 36000ccaaaacgcc aagcgagcag aggaaatcgc aggttgggta gttaaaatct tccgtggctt 36060agactttctt gagtttatgc tgccggatat ctacgctggg gaccgtgcat ccgttaaggc 36120gtttgagatt cattacaccc tacgtggtag tgataagtct ccttctgtat cctgttactc 36180aatcgaagca ggtatgcagg gtgctcgtgc tgatattatt ctagcggatg acgtagagtc 36240gatgcagaat gctcgtacgg cagcgggccg tgccttgctt gaggagctga ctaaggagtt 36300tgaatctatc aaccagtttg gggatatcat ttaccttggt acacctcaga acgtaaactc 36360tatctacaac aacctacctg ctcgtggtta ctctgttcgt atctggactg cgcgttaccc 36420ttcagtagag caagagcaat gttatggcga cttccttgca cctatgattg ttcaagatat 36480gaaggacaac ccagcacttc gctcagggta cgggttggat ggtaatagtg gtgcaccttg 36540tgcccctgaa

atgtatgatg atgaagtcct gattgagaag gaaatctctc agggtgctgc 36600taagttccag cttcagttca tgcttaacac tcgcatgatg gatgctgaca gatacccatt 36660acgcctgaac aatctaatct tcacctcgtt tggtacagag gaagtccctg tgatgcctac 36720gtggagtaat gattccataa acatcattgg tgatgcacct aagtatggta acaagcctac 36780ggatttcatg tacagacctg tagctcgccc atatgaatgg ggtgctgtct cccgcaagat 36840tatgtatatt gaccctgcgg gtggtggtaa gaacggagat gagacgggtg tagccatcgt 36900attcctgcac ggcacattca tttatgtgta tcagtgcttt ggtgtacctg gcggataccg 36960agagtcgtcc ctgaatcgca ttgtgcaggc cgcaaagcag gcgggtgtta aagaggtatt 37020cattgagaag aactttggtc atggcgcgtt tgaggcggta attaagccgt actttgaacg 37080agagtggcct gtaactctgg aagaggatta cgccaccgga cagaaagagt tgcgtatcat 37140tgagacgctg gagccgctca tggcagccca taggcttatc ttcaatgcag agatggtgaa 37200gtcagacttt gagtcggtac agcactatcc gcttgaacta cgcatgtcct acagtctttt 37260caatcaaatg tcgaacataa cgattgagaa gaacagcctc cggcacgatg accgcctaga 37320cgccctgtat ggcgctatac ggcaattaac ttctcagata gactatgacg aggttacacg 37380gattaatcgc ctcagagcgc aggagatgcg cgattacatc catgctatga acacacctca 37440tctacgcagg gcaatgctat atggagatta cggtactgag cgaagagtga ccaacacttc 37500cgtagcgatg cagcagcgag tttacgggca gaactaccga aataaatcgg caagcagaaa 37560tacactttct gcaaggattt caaggactta ttaattactg gacactatag aaggaaggcc 37620cagataataa gagaaaataa taggtaatat atatataggt taacctaggt tatataggta 37680tgccttagta tgggtgtact cctgtacacc ctattcctta ctaccttact atatttacat 37740aataggagag agacaatggc taatgattat agtagtcaac cattaacagg taagtctaag 37800agaaagcagg tacaacctgt aagtgaagaa ctaatgcttc cggtgctcaa aaaagaggaa 37860gttagtaaga aaagcaatgt tattaatgat gccaccaaat caggtaaaca gaaaggggcc 37920atggtgtgcc ttgaagtgaa aggtggtgta ttgaagattg ctatcgcggt tgatggcaaa 37980gaagattcag agtggaagtt agtaacagtg gaaccaactg ttaacccagt ttaagataag 38040gaggaagatt acatggctaa atatggtact acaggttctg ttactggtca ggcttttcga 38100gtaaaagcag tacaaactat tgcaacggca atcccgatgc ctgttgttaa agaagaagac 38160cttaagagta aagaccaccc tatcaacatc aaacatttat caggtaaaca gaaaggtgca 38220atggttgctc ttgagaaagg tgacacaacc ttacatattg ctgttgcacg tggtagtgaa 38280cccacagacc cttgggatgt aactggtatg gaaaaggacg ctgttactcc agcaggggta 38340taataatgct taataaatac ttcaagcgta aagagtttgc ttgccgttgt gggtgcggta 38400catccactgt tgatgctgaa ttactacagg tagtcacaga tgtgcgtgag cactttggtt 38460ctcctgtagt tatcacttcg ggtcatcgct gtgctaagca caatgccaat gtaggtggcg 38520ctaagaactc catgcatctt actggtaagg ctgctgacat taaagtgtct ggcatattac 38580cttctgaagt gcataagtat cttactagca aataccaagg caagtatggt ataggtaagt 38640ataactcctt cactcacatc gatgtacggg atggttgtgc gcgatggtaa gatgtgttga 38700atggtgtgag cgtatggttg cccaagctgc cgaggatggc aactatgatg actggaagaa 38760ctactctgac ttgttagctc aatggaaagg gagatgcaat gaaaaagctg tttaagtcta 38820agaaggttgt aggtgcactg gttgcacttg ttattgctct tgtttctgta ggtcttggtg 38880tagaccttgg ctctggcacg gaatcctctg tgacagatgt ggtctgccaa gtgatcacct 38940gtgaataagt ttctagaagt tctggcaggt cttattggcc tgcttgtctc tgctaagaag 39000aaacaagaag agaaggaggc acaaagtgaa gcgaatcatg ttagtgacaa cccttctgat 39060tggttcgctg accacttccg ggtgtcagca ggcgttacca gagaaagcaa tggtgaaacc 39120tctgaggccg acgctgacgg cagtttacga ggtagacgat aaggtctgct ttagtaagcc 39180tgacgctaca aaacttggtt tgtacattct ctcgctagaa cgcggataca attaatacat 39240agctttatgt atcagtgtct tacgatttac tggacactat agaagaggta agatagcgcc 39300gttcttttga gcggcctatt actagccaat cttcataggg agggttggaa agtaatagga 39360gatagcatgg ctaaattaac caaacctaat actgaaggaa tcttgcataa aggacaatct 39420ttgtatgagt accttgatgc gagagtttta acatcaaagc cgtttggtgc tgcaggtgac 39480gccactactg atgatacgga ggttatagct gcttcattaa actctcagaa agctgtcaca 39540gtctcagatg gtgtattctc tagctctggt attaacagta attactgtaa cttagacggc 39600aggggtagtg gcgtgctaag tcaccgttca agtacaggta actacttagt atttaacaat 39660ctacgtgcag gtcgcttaag taatattacg gtagaaagta ataaggcgac tgatacaact 39720cagggacagc aggtatccct tgctggtgga agtgatgtta ctgtaagtga cgttaacttc 39780tcaaacgtta aaggtactgg tttcagttta atcgcatacc ctaatgatgc gccacctgat 39840ggacttatga ttaaaggcat tcgaggtagc tattccggct atgctactaa taaggcagcc 39900ggatgcgtac ttgctgattc ctcagttaac tccctcatag ataacgtcat tgctaagaac 39960taccctcagt tcggagcagt agagttgaaa ggtacagcca gttacaacat agtcagtaat 40020gttataggga cagattgcca gcatgtaact tacaacggca ctgaagggcc aatagctcct 40080tctaataacc ttatcaaggg ggtgatggct aataacccta agtatgcagc ggttgttgca 40140ggcaaaggaa gtacgaactt aatctcagac gtgctcgtag attactcaac ttctgatgct 40200aggcaggctc atggtgttac agtagagggt tctgataacg tcataaataa tgtgcttatg 40260tcaggatgtg atggtactaa ctctttagga caagggcaga ctgctacaat tgcacgcttt 40320ataggtacag ctaataacaa ctatgcgtct gtatttccta gctacagtgc tacaggtgtt 40380attactttcg aatccggctc tacccgtaac ttcgtagagg taaagcaccc tggcaggaga 40440aacgaccttc tcagttctgc tagtactatt gacggtgcag ctactattga cggcactagt 40500aatagtaacg tagtgcacgc acctgcctta gggcagtaca taggtagtat gtcaggtagg 40560ttcgaatggc ggattaagtc catgtcactc ccttcaggcg ttcttacttc tgctgataag 40620tacagaatgc ttggagatgg tgctgtgtca ttagctgtag gtgggggcac ttcttctcaa 40680gttcgcctat ttacttctga tggtacttct cggacagtgt ccctcaccaa cggtaacgtg 40740cgtctttcta ccagtagcac aggctttttg cagttaggtg ctgatgcaat gaccccagac 40800agtactggta catacgcatt aggttccgcc agccgagcat ggtctggcgg ttttactcaa 40860gcagcattca ctgttacctc agatgctcgg tgtaaaacag aacctcttac tatctcagat 40920gccttactgg atgcttggtc tgaagttgac tttgtgcagt ttcagtattt ggatcgtgtt 40980gaggagaagg gtgcagactc agctagatgg cacttcggta tcatcgctca gcgagctaag 41040gaggctttcg aacgtcacgg tatagatgca catcgctatg gcttcttgtg cttcgacagt 41100tgggatgatg tatacgagga agatgccaat ggctctcgta aactgattac accagcaggt 41160tcccgctacg gtattcgtta cgaggaagta ctgatattag aggctgcgtt gatgcggcgg 41220actattaagc gtatgcagga agcactagct tccctgccta agtaagcaac aggcagtgcg 41280taagcactgc ttttagcgca acttttctta aaggttatca cggtggtagc ctttcagaaa 41340aggaggttac atgattcaaa gactaggttc ttcattagtt aaattcaaga gtaaaatagc 41400aggtgcaatc tggcgtaact tggatgacaa gctcaccgag gttgtatcgc ttaaagattt 41460tggagccaaa ggtgatggta agacaaacga ccaagatgca gtaaatgcag cgatggcttc 41520aggtaagaga attgacggtg ctggtgctac ttacaaagta tcatctttac ctgatatgga 41580gcgattctat aacacccgct tcgtatggga acgtttagca ggtcaacctc tttactatgt 41640gagtaaaggt tttatcaatg gtgaactata taaaatcacg gataaccctt attacaatgc 41700ttggcctcaa gacaaagcgt ttgtatatga gaacgtgata tatgcacctt acatgggtag 41760tgaccgtcat ggtgttagtc gtctgcatgt atcatgggtt aagtctggtg acgatggtca 41820aacatggtct actccagagt ggttaactga tctgcatcca gattacccta cagtgaacta 41880tcattgtatg agtatgggtg tatgtcgcaa ccgtctgttt gccatgattg aaacacgtac 41940tttagccaag aacaaactaa ccaattgtgc attgtgggat cgccctatgt ctcgtagtct 42000gcatcttact ggtggtatca ctaaggctgc aaatcagcaa tatgcaacaa tacatgtacc 42060agatcacgga ctattcgtgg gcgattttgt taacttctct aattctgcgg taacaggtgt 42120atcaggtgat atgactgttg caacggtaat agataaggac aacttcacgg ttcttacacc 42180taaccagcag acttcagatt tgaataacgc tggaaagagt tggcacatgg gtacttcttt 42240ccataagtct ccatggcgta agacagatct tggtctaatc cctagtgtca cagaggtgca 42300tagctttgct actattgata acaatggctt tgttatgggc tatcatcaag gtgatgtagc 42360tccacgagaa gttggtcttt tctacttccc tgatgctttc aatagcccat ctaattatgt 42420tcgtcgtcag ataccatctg agtatgaacc agatgcgtca gagccatgca tcaagtacta 42480tgacggtgta ttatacctta tcactcgtgg cactcttggt gacagacttg gaagctcttt 42540gcatcgtagt agagatatag gtcagacttg ggagtcactg agatttccac ataatgttca 42600tcatactacc ctaccttttg ctaaagtagg agatgacctt attatgtttg gttcagaacg 42660tgcagaaaat gaatgggaag caggtgcacc agatgatcgt tacaaggcat cttatcctcg 42720taccttctat gcacgattga atgtaaacaa ttggaatgca gatgatattg aatgggttaa 42780catcacagac caaatctatc aaggtgacat tgtgaactct agtgtaggtg taggttcggt 42840agtagttaaa gacagctaca tttactatat ctttggtggc gaaaaccatt tcaacccaat 42900gacttatggt gacaacaaag gtaaagaccc atttaaaggt catggacacc ctactgatat 42960atactgctat aagatgcaga ttgcaaatga caatcgtgta tctcgtaagt ttacatatgg 43020tgcaactccg ggtcaagcta tacctacttt catgggtact gatggaatac gaaatatccc 43080tgcacctttg tatttctcag ataacattgt tacagaggat actaaagttg gacacttaac 43140acttaaagca agcacaagtt ccaatatacg atctgaagtg cagatggaag gtgaatatgg 43200ctttattggc aagtctgttc caaaggacaa cccaactggt caacgtttga ttatttgtgg 43260tggagaagag acttcgtcct cttcaggtgc acagataact ttgcacggct ctaattcaag 43320taaggctaat cgtatcactt ataacggaaa tgagcaccta ttccaaggtg caccaatcat 43380gcctgctgta gataaccagt ttgctgctgg tggacctagt aaccgattca ctaccatcta 43440cctaggtagt gaccctgtta caacttcaga tgctgaccac aagtacagta tctctagtat 43500taataccaag gtgttaaagg cttggagcag ggttggtttt aaacagtatg gtttgaatag 43560tgaagcagag agggaccttg atagcataca cttcggtgtc ttggctcagg atattgtagc 43620tgcttttgaa gctgaagggt tggatgccat taagtatgga attgtgtcct tcgaagaagg 43680taggtacggt gtgaggtata gtgaagttct aatactagag gctgcttata ctcgttatcg 43740tttagacaag ttagaggaga tgtatgccac taataaaatc agttaagcaa gctgctgtac 43800tccagaacac agaagagctt attcaatcag gacgtgaccc taagcaggct tatgccattg 43860ccaaggatgt tcaacgtcgt gccatgaaga aaccttctgc atcttctgcg taagcaggtt 43920aatatcttag tataaacaag ggcagactta ggtttgtcct tagtgtattc caaaggaggt 43980aacatgctga aagatggttg ggtttcatat gaccctacag accctaagaa ttggctacag 44040gttatcgcta tagcttgtgc aggtagccta ttggctgccc tgatgtattc attatggatg 44100tacacaaagt aaccaaagtc aaaattttga tgtaggcgtg tgtcagctct ctcgccctcg 44160ccctcgccgg gttgtcccca tagggtggcc tgagggaatc cgtcttcgac gggcagggct 44220gatgtactcc ttgtctagta caagggaggc ggagggaacg cctagggagg cctaggaatg 44280gcttagtggt ggacaaggtg attaccttag tgaagcctct tagtgcattc ctgaggccat 44340tcagggcgtt tatgagggat tgacagggtg tgagggcgtg ggcta 44385460DNAArtificial SequenceSynthetic Polynucleotide 4gtttttgaac acacatgaac aaggaagtac aggtctcaca gtgtacggac ctaaagttcc 60530DNAArtificial SequenceSynthetic Polynucleotide 5ttacgcgaac gcgaagtccg actctaagat 30630DNAArtificial SequenceSynthetic Polynucleotide 6ccagttgcac gagtctcaat tggacaaaat 30730DNAArtificial SequenceSynthetic Polynucleotide 7tcagtggcaa atcgcccaat taggacccat 30824DNAArtificial SequenceSynthetic Polynucleotide 8ccgaaggtaa gatgggtcct aatt 24930DNAArtificial SequenceSynthetic Polynucleotide 9ttaaataccg gaacttctcc gtaagtagtt 301030DNAArtificial SequenceSynthetic Polynucleotide 10gttcaacact gtatacatct tgtcagatga 301160DNAArtificial SequenceSynthetic Polynucleotide 11gaaatgtgcg cggaacccct atttgtttat agggacacag agagacactc aaggtaacac 601260DNAArtificial SequenceSynthetic Polynucleotide 12cagtatgata gtacatctct atgtgtccct tgtctcatga gcggatacat atttgaatgt 601360DNAArtificial SequenceSynthetic Polynucleotide 13gggggtactt tgggttcttg aactatgaga ccttgttcat gtgtgttcaa aaacgttata 601460DNAArtificial SequenceSynthetic Polynucleotide 14gtgttacctt gagtgtctct ctgtgtccct tgtctcatga gcggatacat atttgaatgt 601560DNAArtificial SequenceSynthetic Polynucleotide 15gggggaactt taggtccgta cactgtgaga ccttgttcat gtgtgttcaa aaacgttata 601660DNAArtificial SequenceSynthetic Polynucleotide 16tcctgtcggg tggtggtgcg ggagtggcta tgtctcatga gcggatacat atttgaatgt 601760DNAArtificial SequenceSynthetic Polynucleotide 17ggaagggtgg gctgatcaga gtcgggaggg ccttgttcat gtgtgttcaa aaacgttata 601830DNAArtificial SequenceSynthetic Polynucleotide 18tgtctcatga gcggatacat atttgaatgt 301930DNAArtificial SequenceSynthetic Polynucleotide 19ccttgttcat gtgtgttcaa aaacgttata 302030DNAArtificial SequenceSynthetic Polynucleotide 20cctgtacttc cttgttcatg tgtgttcaaa 302130DNAArtificial SequenceSynthetic Polynucleotide 21ataaacaaat aggggttccg cgcacatttc 302260DNAArtificial SequenceSynthetic Polynucleotide 22tataacgttt ttgaacacac atgaacaagg tctcatagtt caagaaccca aagtaccccc 602330DNAArtificial SequenceSynthetic Polynucleotide 23acggaacctc cttcttgggt tctttgacgc 302430DNAArtificial SequenceSynthetic Polynucleotide 24ccagtggctg gcgtcaaaga acccaagaag 302530DNAArtificial SequenceSynthetic Polynucleotide 25ggaagtcggt tcatcgctaa gcacgattgc 302630DNAArtificial SequenceSynthetic Polynucleotide 26tggcgatgat gcaatcgtgc ttagcgatga 302730DNAArtificial SequenceSynthetic Polynucleotide 27gatgcaacgt tcagcgcagc actttcggca 302830DNAArtificial SequenceSynthetic Polynucleotide 28ttgtagttgg tgccgaaagt gctgcgctga 302960DNAArtificial SequenceSynthetic Polynucleotide 29acattcaaat atgtatccgc tcatgagaca agggacacat agagatgtac tatcatactg 603030DNAArtificial SequenceSynthetic Polynucleotide 30aacagcgtcg cggtcatcca cagcgttcgc 303160DNAArtificial SequenceSynthetic Polynucleotide 31gcgaacgctg tggatgaccg cgacgctgtt ccgtttggtc aactaaagac catgaaccag 603260DNAArtificial SequenceSynthetic Polynucleotide 32gtggacttaa agtagttcct ttgatgctta ttactcgttc tccaccatga ttgcattagg 603360DNAArtificial SequenceSynthetic Polynucleotide 33cctaatgcaa tcatggtgga gaacgagtaa taagcatcaa aggaactact ttaagtccac 60346021DNAArtificial SequenceSynthetic Polynucleotide 34tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 60cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 120gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 180tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 240agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 300cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 360ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 420tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 480gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 540gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 600gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 660ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 720ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 780ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 840gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 900ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 960tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1020ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1080gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1140tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1200cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1260ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1320gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1380caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1440gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1500ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1560tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 1620caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 1680tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 1740cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 1800ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 1860aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 1920tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 1980gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 2040gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt 2100tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag 2160tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta 2220tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag 2280accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta 2340tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt 2400tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac 2460tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta 2520ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa 2580accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 2640gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 2700gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 2760ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 2820catatcgact acgtcgtaag gccgtttctg acagagtaaa attcttgagg gaactttcac 2880cattatggga aatggttcaa gaaggtattg acttaaactc catcaaatgg tcaggtcatt 2940gagtgttttt tatttgttgt attttttttt ttttagagaa aatcctccaa tatcaaatta 3000ggaatcgtag tttcatgatt ttctgttaca cctaactttt tgtgtggtgc cctcctcctt 3060gtcaatatta atgttaaagt gcaattcttt ttccttatca cgttgagcca ttagtatcaa 3120tttgcttacc tgtattcctt tactatcctc ctttttctcc ttcttgataa atgtatgtag 3180attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt 3240tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt 3300ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt 3360ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc 3420aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa 3480gatagtggcg atagggtcaa ccttattctt tggcaaatct ggagcagaac cgtggcatgg 3540ttcgtacaaa

ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa 3600caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt 3660gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga 3720atcaatcaat tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg tttcctccac 3780agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg 3840caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc 3900tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt 3960accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa 4020ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa 4080gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact 4140accggtaccc catttaggac cacccacagc acctaacaaa acggcatcaa ccttcttgga 4200ggcttccagc gcctcatctg gaagtgggac acctgtagca tcgatagcag caccaccaat 4260taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac 4320cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt 4380cttaggggca gacatagggg cagacattag aatggtatat ccttgaaata tatatatata 4440ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa ccacctattg 4500gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat gcaagcattt 4560agtcatgaac gcttctctat tctatatgaa aagccggttc cggcctctca cctttccttt 4620ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa 4680aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt 4740tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt 4800attcccacag ttaactgcgg tcaagatatt tcttgaatca ggcgccttag accgctcggc 4860caaacaacca attacttgtt gagaaataga gtataattat cctataaata taacgttttt 4920gaacacacat gaacaaggaa gtacaggaca attgattttg aagagaatgt ggattttgat 4980gtaattgttg ggattccatt tttaataagg caataatatt aggtatgtgg atatactaga 5040agttctcctc gaccgtcgat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 5100accgcatcag gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta 5160aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 5220atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 5280cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 5340accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 5400taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 5460agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 5520cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc 5580attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 5640gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 5700gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga 5760attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct 5820gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt 5880gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg 5940tgtgaaattg ttatccgctc acaattccac acaacatagg agccggaagc ataaagtgta 6000aagcctgggg tgcctaatga g 60213560DNAArtificial SequenceSynthetic Polynucleotide 35tataacgttt ttgaacacac atgaacaagg tctcacagtg tacggaccta aagttccccc 603630DNAArtificial SequenceSynthetic Polynucleotide 36attacgcgat gacagtagac aacctttccg 303730DNAArtificial SequenceSynthetic Polynucleotide 37tgcagcaata ccggaaaggt tgtctactgt 303830DNAArtificial SequenceSynthetic Polynucleotide 38atatgtctcc tcatagatgt gcctatgtgg 303930DNAArtificial SequenceSynthetic Polynucleotide 39acttgtgact ccacataggc acatctatga 304030DNAArtificial SequenceSynthetic Polynucleotide 40gaataacctg agggtcaata ccctgcttgt 304130DNAArtificial SequenceSynthetic Polynucleotide 41gacatgatgg acaagcaggg tattgaccct 304260DNAArtificial SequenceSynthetic Polynucleotide 42acattcaaat atgtatccgc tcatgagaca agggacacag agagacactc aaggtaacac 604330DNAArtificial SequenceSynthetic Polynucleotide 43aacagcatcg cggtcatcca cggcgttcgc 304460DNAArtificial SequenceSynthetic Polynucleotide 44gcgaacgccg tggatgaccg cgatgctgtt ccgtttggtc aacttaagac catgaaccag 604560DNAArtificial SequenceSynthetic Polynucleotide 45gactacacgt ctttccttgt gatttaccaa ttacacgtcc tctacggcta ttgctgttgg 604660DNAArtificial SequenceSynthetic Polynucleotide 46ccaacagcaa tagccgtaga ggacgtgtaa ttggtaaatc acaaggaaag acgtgtagtc 604760DNAArtificial SequenceSynthetic Polynucleotide 47tttgaacaca catgaacaag gaagtacagg tctctcggcc tcggcctcgc cgggatgtcc 604830DNAArtificial SequenceSynthetic Polynucleotide 48cgtcctgatg tactggtagg tgagtgcgga 304930DNAArtificial SequenceSynthetic Polynucleotide 49atttggtgga tgaaggaagg gccgacgaat 305030DNAArtificial SequenceSynthetic Polynucleotide 50ttctccgtgt agttatagcc tttccatata 305130DNAArtificial SequenceSynthetic Polynucleotide 51cggcttgctt tttgagaagg cattccccga 305230DNAArtificial SequenceSynthetic Polynucleotide 52aagataataa ctttgaggta atctttcatc 305330DNAArtificial SequenceSynthetic Polynucleotide 53agattatgtg tatggtcgtg atgtcaaaat 305430DNAArtificial SequenceSynthetic Polynucleotide 54ctggaacctt agctgcctca atgcgaggtg 305530DNAArtificial SequenceSynthetic Polynucleotide 55catttcaagc agtaggtctg gcacaaaagg 305630DNAArtificial SequenceSynthetic Polynucleotide 56cttgtttgtc aaagatttca ggtacttgac 305730DNAArtificial SequenceSynthetic Polynucleotide 57aggaggagta tttcttcata atgaagaagg 305830DNAArtificial SequenceSynthetic Polynucleotide 58ccacatacgc atctgattag cttcaaagtt 305930DNAArtificial SequenceSynthetic Polynucleotide 59gcagttaaag agcgcgatga agcgaagaag 306030DNAArtificial SequenceSynthetic Polynucleotide 60tcaatcctcc aataagtcta cgctggcctt 306130DNAArtificial SequenceSynthetic Polynucleotide 61gcaaatacga ttggtgtagg tcagatgacc 306230DNAArtificial SequenceSynthetic Polynucleotide 62taaacctcct attactatcc agccctcccc 306330DNAArtificial SequenceSynthetic Polynucleotide 63ttgagcggcc tattactcac cagtcttcac 306460DNAArtificial SequenceSynthetic Polynucleotide 64gaaatgtgcg cggaacccct atttgtttat tagcccacgc ccacacacgc tgtcaagcgg 606557DNAArtificial SequenceSynthetic Polynucleotide 65gtttttgaac acacatgaac aaggaagtac aggtcgccct cgccctcgcc gggttgt 576630DNAArtificial SequenceSynthetic Polynucleotide 66ggagagtcag agggcttaag gtttactgct 306730DNAArtificial SequenceSynthetic Polynucleotide 67tgctatgcta cgcgatgcag taggtgcgaa 306830DNAArtificial SequenceSynthetic Polynucleotide 68cagggtcacg catctcatat gggtcgaaga 306930DNAArtificial SequenceSynthetic Polynucleotide 69tggacttgct caccactgag gagttcctct 307030DNAArtificial SequenceSynthetic Polynucleotide 70gctttgtcag cctgctcagg gaagcaagca 307130DNAArtificial SequenceSynthetic Polynucleotide 71taacttcgct gctggtctgg agttcgctcg 307230DNAArtificial SequenceSynthetic Polynucleotide 72tgtgcacttt gttctgcatt ccatgaggct 307330DNAArtificial SequenceSynthetic Polynucleotide 73tgtgcatctc ttaatagaga cccaccactc 307430DNAArtificial SequenceSynthetic Polynucleotide 74aagaagctga gtggctatct gctgcgcagt 307530DNAArtificial SequenceSynthetic Polynucleotide 75tctaaggatg cagatcagac taagctagcc 307630DNAArtificial SequenceSynthetic Polynucleotide 76gccttagctc gtaactcttc ttccgcaata 307730DNAArtificial SequenceSynthetic Polynucleotide 77taaaaccgaa gtgtcagact taggtaaagc 307830DNAArtificial SequenceSynthetic Polynucleotide 78tattgccgcc ccagcttaca ttctgtttaa 307930DNAArtificial SequenceSynthetic Polynucleotide 79ttgacgggtt ttatccagaa ggatacttca 308030DNAArtificial SequenceSynthetic Polynucleotide 80gctatctcct attactttcc aaccctccct 308130DNAArtificial SequenceSynthetic Polynucleotide 81ttgagcggcc tattactagc caatcttcat 308260DNAArtificial SequenceSynthetic Polynucleotide 82gaaatgtgcg cggaacccct atttgtttat tagcccacgc cctcacaccc tgtcaatccc 608360DNAArtificial SequenceSynthetic Polynucleotide 83tataacgttt ttgaacacac atgaacaagg tctcacagtt tacacttttg gttatccccc 608430DNAArtificial SequenceSynthetic Polynucleotide 84attagaagtc atcgtcttct tcggcttcgc 308530DNAArtificial SequenceSynthetic Polynucleotide 85agcggacgaa tctcgcagcc gtaaacctca 308630DNAArtificial SequenceSynthetic Polynucleotide 86tcatcacctt cgagggcctt aagggctgac 308730DNAArtificial SequenceSynthetic Polynucleotide 87attgccgcat ggtcagccct taaggccctc 308830DNAArtificial SequenceSynthetic Polynucleotide 88catcgtgtcc ttgaacacat cgtacccatc 308930DNAArtificial SequenceSynthetic Polynucleotide 89cggggacgct gctgaggctc agattcagaa 309060DNAArtificial SequenceSynthetic Polynucleotide 90acattcaaat atgtatccgc tcatgagaca agggacacag agacatcaac atatagtgtc 60

Claims

1-27. (canceled)

28. A composition comprising viable recombinant bacteriophages that each comprise (a) a capsid head consisting of proteins encoded by genes of one species of bacteriophage; and (b) a heterologous tail consisting of proteins encoded by genes of another species of bacteriophage, wherein the species of bacteriophage of (a) is different from the species of bacteriophage of (b), and the composition comprises viable recombinant bacteriophages comprising heterologous tails from at least two different species of bacteriophages.

29. The composition of claim 28, wherein the capsid head consists of proteins encoded by genes of a bacteriophage selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae and Guttavirus.

30. The composition of claim 28, wherein the heterologous tail consists of proteins encoded by genes of a bacteriophage selected from the group consisting of: Myoviridae, Siphoviridae, Podoviridae, Tectiviridae, Corticoviridae, Lipothrixviridae, Plasmaviridae, Rudiviridae, Fuselloviridae, Inoviridae, Microviridae, Leviviridae, Cystoviridae, Ampullaviridae, Bicaudaviridae, Clavaviridae, Globuloviridae and Guttavirus.

31. The composition of claim 28, wherein the bacteriophage of (a) is a T3 bacteriophage, T4 bacteriophage, T5 bacteriophage, T7 bacteriophage, K1F bacteriophage, K11 bacteriophage, YppR bacteriophage or a SP6 bacteriophage.

32. The composition of claim 28, wherein the bacteriophage of (b) is a T3 bacteriophage, T4 bacteriophage, T5 bacteriophage, T7 bacteriophage, K1F bacteriophage, K11 bacteriophage, YppR bacteriophage or SP6 bacteriophage.

33. The composition of claim 32, wherein the capsid head consists of proteins encoded by genes of a T7 bacteriophage.

34. The composition of claim 32, wherein the capsid head consists of proteins encoded by genes of a K11 bacteriophage.

35. The composition of claim 28, wherein the heterologous tail comprises host recognition elements.

36. The composition of claim 35, wherein the host recognition elements comprise tail fibers or tail spikes.

37. The composition of claim 28, wherein the heterologous tail comprises a portal complex through which the heterologous tail attaches to the capsid head.

38. The composition of claim 28, wherein the composition comprises viable recombinant bacteriophages comprising heterologous tails from at least three different species of bacteriophages.

39. The composition of claim 28, further comprising a pharmaceutical carrier and/or excipient.