U.S. patent application number 15/426908 was filed with the patent office on 2017-05-25 for compositions for administration of zoledronic acid or related compounds for treating low back pain.
The applicant listed for this patent is ANTECIP BIOVENTURES II LLC. Invention is credited to Herriot Tabuteau.
Application Number | 20170143747 15/426908 |
Document ID | / |
Family ID | 50100459 |
Filed Date | 2017-05-25 |
United States Patent
Application |
20170143747 |
Kind Code |
A1 |
Tabuteau; Herriot |
May 25, 2017 |
COMPOSITIONS FOR ADMINISTRATION OF ZOLEDRONIC ACID OR RELATED
COMPOUNDS FOR TREATING LOW BACK PAIN
Abstract
Oral dosage forms of osteoclast inhibitors, such as zoledronic
acid in an acid or a salt form, can be used to treat or alleviate
pain or related conditions, such as low back pain.
Inventors: |
Tabuteau; Herriot; (New
York, NY) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
ANTECIP BIOVENTURES II LLC |
New york |
NY |
US |
|
|
Family ID: |
50100459 |
Appl. No.: |
15/426908 |
Filed: |
February 7, 2017 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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15083105 |
Mar 28, 2016 |
9616077 |
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15426908 |
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15042017 |
Feb 11, 2016 |
9585901 |
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15083105 |
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14279226 |
May 15, 2014 |
9265778 |
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15042017 |
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14063979 |
Oct 25, 2013 |
8802658 |
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14279226 |
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13894274 |
May 14, 2013 |
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14063979 |
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15386858 |
Dec 21, 2016 |
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13894274 |
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15352461 |
Nov 15, 2016 |
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15386858 |
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PCT/US2015/032739 |
May 27, 2015 |
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15352461 |
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61646538 |
May 14, 2012 |
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61647478 |
May 15, 2012 |
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61654292 |
Jun 1, 2012 |
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61654383 |
Jun 1, 2012 |
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61655527 |
Jun 5, 2012 |
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61655541 |
Jun 5, 2012 |
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61764563 |
Feb 14, 2013 |
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61762225 |
Feb 7, 2013 |
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61767647 |
Feb 21, 2013 |
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61767676 |
Feb 21, 2013 |
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61803721 |
Mar 20, 2013 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A61K 31/675 20130101;
A61K 9/0053 20130101; A61K 9/20 20130101; A61K 31/663 20130101;
A61K 9/28 20130101; A61K 47/12 20130101; A61K 9/2009 20130101; A61K
9/2027 20130101; A61K 45/06 20130101; A61K 9/2004 20130101; A61K
9/2013 20130101; A61K 9/2054 20130101 |
International
Class: |
A61K 31/675 20060101
A61K031/675; A61K 9/20 20060101 A61K009/20; A61K 9/00 20060101
A61K009/00 |
Claims
1. A method of treating low back pain, comprising orally
administering zoledronic acid in a disodium salt form to a human
being in need thereof, wherein the zoledronic acid in the disodium
salt form is orally administered in a manner that results in an AUC
of zoledronic acid, over a four week period, that is about 100
ngh/mL to about 1000 ngh/mL.
2. The method of claim 1, wherein each orally administered dose
contains about 50 mg to about 60 mg of the zoledronic acid in the
disodium salt form.
3. The method of claim 1, wherein the zoledronic acid in the
disodium salt form is orally administered at an interval of about 5
to 10 days.
4. The method of claim 2, wherein the zoledronic acid in the
disodium salt form is orally administered about once a week.
5. The method of claim 1, wherein each orally administered dose of
the zoledronic acid in the disodium salt form is administered in a
manner that provides an AUC of zoledronic acid of about 140 ngh/mL
to about 150 ngh/mL, measured over a 24 hour period.
6. The method of claim 4, wherein each orally administered dose of
the zoledronic acid in the disodium salt form is administered in a
manner that provides an AUC of zoledronic acid of about 130 ngh/mL
to about 150 ngh/mL, measured over a 24 hour period.
7. The method of claim 1, wherein the low back pain is associated
with a Modic change.
8. The method of claim 6, wherein the low back pain is associated
with a Modic change.
9. The method of claim 1, wherein the zoledronic acid in a disodium
salt form is orally administered in a dosage form that contains no
external agents which enhance the bioavailability of zoledronic
acid.
10. A method of treating low back pain, comprising orally
administering zoledronic acid in a disodium salt form to a human
being in need thereof, wherein the zoledronic acid in the disodium
salt form is orally administered in a manner that results in an AUC
of zoledronic acid that is about 100 ngh/mL to about 500 ngh/mL,
measured over a 24 hour period, each time the zoledronic acid in
the disodium salt form is administered.
11. The method of claim 10, wherein the zoledronic acid in the
disodium salt form is orally administered in an amount that results
in an AUC of zoledronic acid that is about 100 ngh/mL to about 160
ngh/mL, measured over a 24 hour period, each time the zoledronic
acid in the disodium salt form is administered.
12. The method of claim 10, wherein about 50 mg to about 70 mg of
the zoledronic acid in the disodium salt form is orally
administered weekly.
13. The method of claim 10, wherein the zoledronic acid in the
disodium salt form is orally administered about once a week for
about six weeks.
14. The method of claim 12, wherein the zoledronic acid in the
disodium salt form is orally administered about once a week for
about six weeks.
15. The method of claim 10, wherein the low back pain is associated
with a Modic change.
16. The method of claim 10, wherein the zoledronic acid in a
disodium salt form is orally administered in a dosage form that
contains no external agents which enhance the bioavailability of
zoledronic acid
17. A method of treating low back pain, comprising orally
administering zoledronic acid in a disodium salt form to a human
being in need thereof, wherein about 100 mg to about 400 mg of the
zoledronic acid in the disodium salt form is orally administered in
a manner that results in a bioavailability of zoledronic acid in
the human being of about 1.2% to about 3.5%.
18. The method of claim 17, wherein the zoledronic acid in the
disodium salt form is orally administered in an amount and
frequency that results in a bioavailability of zoledronic acid in
the human being of about 1.5% to about 2.5%.
19. The method of claim 17, wherein the human being is not
suffering from cancer or osteoporosis.
20. The method of claim 17, wherein the human being is not
suffering from multiple myeloma.
21. The method of claim 17, wherein the human being is not
suffering from bone metastases.
22. The method of claim 17, wherein the human being is not
suffering from a solid tumor.
23. The method of claim 17, wherein the human being is not
suffering from bone metastases from a solid tumor.
24. The method of claim 17, wherein the human being is not
suffering from a blood cancer.
25. The method of claim 17, wherein the human being is not
suffering from a leukemia.
26. The method of claim 17, wherein the human being is not
suffering from a giant cell tumor of bone.
27. The method of claim 17, wherein the human being is not
suffering from hypercalcemia of malignancy.
28. The method of claim 17, wherein the zoledronic acid in a
disodium salt form is orally administered in a dosage form that
contains no external agents which enhance the bioavailability of
zoledronic acid.
29. The method of claim 17, wherein the zoledronic acid is orally
administered in a dosage form that further comprises silicified
microcrystalline cellulose, a crosslinked polyvinylpyrrolidone, a
fumed silica, and magnesium stearate.
30. The method of claim 17, wherein the low back pain is associated
with a Modic change.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent
application Ser. No. 15/083,105, filed Mar. 28, 2016, which is a
continuation of U.S. patent application Ser. No. 15/042,017, filed
Feb. 11, 2016, which is a divisional of U.S. patent application
Ser. No. 14/279,226, filed May 15, 2014, now U.S. Pat. No.
9,265,778, which is a continuation of U.S. patent application Ser.
No. 14/063,979, filed Oct. 25, 2013, now U.S. Pat. No. 8,802,658,
which is a continuation-in-part of U.S. patent application Ser. No.
13/894,274, filed May 14, 2013, now abandoned, which claims the
benefit of U.S. Provisional Pat. App. Nos. 61/646,538, filed May
14, 2012; 61/647,478, filed May 15, 2012; 61/654,292, filed Jun. 1,
2012; 61/654,383, filed Jun. 1, 2012; 61/655,527, filed Jun. 5,
2012; 61/655,541, filed Jun. 5, 2012; 61/764,563, filed Feb. 14,
2013; 61/762,225, filed Feb. 7, 2013; 61/767,647, filed Feb. 21,
2013; 61/767,676, filed Feb. 21, 2013; and 61/803,721, filed Mar.
20, 2013; this application is also a continuation-in-part of U.S.
patent application Ser. No. 15/386,858, filed Dec. 21, 2016, which
is a continuation-in-part of a U.S. patent application Ser. No.
15/352,461, filed Nov. 15, 2016, which is a continuation-in-part of
International Pat. App. No. PCT/US2015/032739, filed May 27, 2015;
any of the applications, U.S. patents issued from, or U.S.
publications of any of the above applications are incorporated by
references in their entirety.
SUMMARY
[0002] Bisphosphonate compounds are potent inhibitors of osteoclast
activity, and are used clinically to treat bone-related conditions
such as osteoporosis and Paget's disease of bone; and
cancer-related conditions including multiple myeloma, and bone
metastases from solid tumors. They generally have low oral
bioavailability.
[0003] Patchy osteoporosis and bone marrow edema may result from
osteoclast hyperactivity. Zoledronic acid is a potent inhibitor of
bone resorption and osteoclast activity. Nitrogen containing
bisphosphonates, such as zoledronic acid, also inhibit the
mevalonate pathway in the osteoclast thereby interrupting normal
osteoclast function.
[0004] It has been discovered that oral dosage forms of
bisphosphonate compounds, such as zoledronic acid, can be used to
treat or alleviate pain or related conditions.
[0005] Some embodiments include a method of enhancing the oral
bioavailability of zoledronic acid comprising orally administering
a dosage form containing zoledronic acid in the disodium salt
form.
[0006] Some embodiments include a dosage form comprising zoledronic
acid in the disodium salt form, wherein the bioavailability, in a
mammal, of zoledronic acid in the disodium salt form is greater
than the bioavailability of zoledronic acid in the diacid form
would be in the same dosage form.
[0007] Some embodiments include a dosage form comprising zoledronic
acid in an acid or a salt form, such as the disodium salt form,
wherein the dosage form contains an amount of zoledronic acid in
the disodium salt form that provides an area under the plasma
concentration curve of zoledronic acid of about 4 ngh/mL to about
2000 ngh/mL to a human being to which the dosage form is
administered.
[0008] Some embodiments include a dosage form comprising zoledronic
acid in the disodium salt form, wherein the disodium salt form is
present in a lower molar amount than would be present if the
zoledronic acid were in the diacid form; and wherein the zoledronic
acid in the disodium salt form has an improved bioavailability as
compared to the zoledronic acid in the diacid form to the extent
that the lower molar amount of the disodium salt in the dosage form
does not reduce the amount of zoledronic acid delivered to the
plasma of a mammal.
[0009] Although an oral dosage form with enhanced bioavailability
with respect to the bisphosphonate compound can be used, the
treatment can also be effective using an oral dosage form that
includes a bisphosphonate compound, such as zoledronic acid,
wherein the bioavailability of the bisphosphonate is unenhanced, or
is substantially unenhanced.
[0010] Some embodiments include a method of relieving inflammatory
pain comprising administering an oral dosage form containing
zoledronic acid to a mammal in need thereof, wherein the mammal
experiences significant pain relief more than 3 hours after
administration of the dosage form.
[0011] Some embodiments include a method of relieving pain
associated with an arthritis comprising administering an oral
dosage form containing zoledronic acid to a human being in need
thereof.
[0012] Some embodiments include a method of treating complex
regional pain syndrome comprising administering an oral dosage form
containing zoledronic acid to a mammal in need thereof.
[0013] Some embodiments include an oral dosage form comprising
zoledronic acid, wherein the oral bioavailability of zoledronic
acid is substantially unenhanced. For example, in some embodiments,
the oral bioavailability in the dosage form is about 0.01% to about
4%.
[0014] Some embodiments include a pharmaceutical product comprising
more than one unit of an oral dosage form described herein. In some
embodiments, each unit of the oral dosage form contains about 1 mg
to about 50 mg of zoledronic acid.
[0015] Some embodiments include a method of relieving inflammatory
pain comprising administering an oral dosage form containing
zoledronic acid to a mammal in need thereof.
[0016] In some embodiments, the mammal receives a total monthly
dose of zoledronic acid that is about 800 mg/m.sup.2 or less.
[0017] In some embodiments, the dosage form contains about 10
mg/m.sup.2 to about 20 mg/m.sup.2 based upon the body surface area
of the mammal.
[0018] Some embodiments include a method of relieving inflammatory
pain comprising orally administering zoledronic acid to a mammal in
need thereof.
[0019] In some embodiments, about 300 mg/m.sup.2 to about 600
mg/m.sup.2 of zoledronic acid is administered per month, based upon
the body surface area of the mammal.
[0020] In some embodiments, about 50 mg/m.sup.2 to about 600
mg/m.sup.2 of zoledronic acid is administered per month, based upon
the body surface area of the mammal.
[0021] Some embodiments include administering an osteoclast
inhibitor, such as a bisphosphonate, including zoledronic acid,
neridronic acid, etc. to inhibit the development of pain,
unweighting, and edema when administered early such as when a
precipitating event such as fracture occurs, wherein the
precipitating event is associated with CRPS.
[0022] Some embodiments include administering an osteoclast
inhibitor, such as a bisphosphonate, including zoledronic acid,
neridronic acid, etc. to reverse established allodynia and
unweighting when administered at least 4 weeks after a
precipitating event such as fracture that is associated with
CRPS.
BRIEF DESCRIPTION OF DRAWINGS
[0023] FIG. 1 is a plot of pain compression thresholds in a rat
model of inflammatory pain using three different doses of
zoledronic acid. Measurements were taken at baseline (BL) and at
various time points after dosing on the days indicated.
[0024] FIG. 2A is a graph depicting reversal of arthritis pain for
two different doses of zoledronic acid in a rat model of arthritis
pain.
[0025] FIG. 2B is a graph depicting pain thresholds for two
different doses of zoledronic acid in a rat model of arthritis
pain.
[0026] FIG. 3 is a graph summarizing the results for vehicle and
zoledronic acid treated rats in a rat model of complex regional
pain syndrome.
[0027] FIG. 4 depicts hindpaw pain thresholds for vehicle and
zoledronic acid treated rats in a rat model of complex regional
pain syndrome.
[0028] FIG. 5 depicts weight bearing for vehicle and zoledronic
acid treated rats in a rat model of complex regional pain
syndrome.
[0029] FIG. 6 depicts paw thickness change for vehicle and
zoledronic acid treated rats in a rat model of complex regional
pain syndrome.
[0030] FIG. 7 depicts the aqueous solubility of disodium
zoledronate tetrahydrate as compared to the diacid form of
zoledronic acid.
[0031] FIG. 8 depicts the plasma concentration of zoledronic acid
in dogs over time after administration of 150 mg of the disodium
salt form of zoledronic acid and the diacid form of zoledronic
acid.
[0032] FIG. 9 depicts the compressibility of dosage forms
containing zoledronic acid in the disodium salt form as compared to
the diacid form.
[0033] FIG. 10 depicts the change in VAS pain score compared to
placebo at three months with zoledronic acid treatment in patients
with osteoarthritis of the knee, bone marrow lesions, and different
degrees of joint space narrowing.
[0034] FIG. 11 depicts the change in VAS pain score compared to
baseline at three months with zoledronic acid treatment in patients
with osteoarthritis of the knee, bone marrow lesions, and different
degrees of joint space narrowing.
[0035] FIG. 12 depicts the change in VAS pain score compared to
placebo at three months with zoledronic acid treatment in different
subgroups of patients with osteoarthritis of the knee and bone
marrow lesions.
[0036] FIG. 13 depicts the change in BML lesion size compared to
placebo at six months with zoledronic acid treatment in patients
with osteoarthritis of the knee, bone marrow lesions, and different
degrees of joint space narrowing.
[0037] FIG. 14 depicts hindpaw pain thresholds for vehicle and
zoledronic acid treated rats in a rat model of complex regional
pain syndrome.
[0038] FIG. 15 depicts weight bearing for vehicle and zoledronic
acid treated rats in a rat model of complex regional pain
syndrome.
[0039] FIG. 16 depicts hindpaw pain thresholds for rats
administered zoledronic acid at the time of fracture as compared to
rats administered zoledronic acid four weeks after fracture.
DETAILED DESCRIPTION
[0040] Inhibitors of osteoclast activity include bisphosphonate
compounds such as pamidronate or pamidronic acid, neridronate or
neridronic acid, olpadronate or olpadronic acid, alendronate or
alendronic acid, incadronate or incadronic acid, ibandronate or
ibandronic acid, risedronate or risedronic acid, cimadronate or
cimadronic acid, zoledronate or zoledronic acid, etidronate or
etidronic acid, clodronate or clodronic acid, tiludronate or
tiludronic acid, etc.
[0041] RANK/RANKL antagonists may be inhibitors of osteoclast
activity. RANK/RANKL antagonists include but are not limited to OPG
(osteoprotegerin) or a variant thereof, an anti-RANKL antibody such
as denosumab, a monoclonal anti-RANKL antibody, a small interfering
RNA, a microRNA, a precursor molecule, a ribozyme, an antisense
nucleic acid, or an aptamer targeting RANKL. Antibodies such as
AB-25E9, small molecules, small interfering RNAs, microRNAs,
precursor molecules, ribozymes, antisense nucleic acids, or
aptamers that target the cell-surface protein Siglec-15 may be
osteoclast inhibitors.
[0042] Some Bruton's tyrosine kinase (BTK) inhibitors may be
inhibitors of osteoclast activity. BTK inhibitors can include
ONO-4059; ibrutinib; Benzo[b]thiophene-2-carboxamide,
N-[3-[6-[[4-[(2R)-1,4-dimethyl-3-oxo-2-piperazinyl]phenyl]amino]-4,5-dihy-
dro-4-methyl-5-oxo-2-pyrazinyl]-2-methylphenyl]-4,5,6,7-tetrahydro-
(GDC-0834); RN-486; Benzamide,
4-(1,1-dimethylethyl)-N-[3-[8-(phenylamino)imidazo[1,2-a]pyrazin-6-yl]phe-
nyl]-(CGI-560); Benzamide,
N-[3-[4,5-dihydro-4-methyl-6-[[4-(4-morpholinylcarbonyl)phenyl]amino]-5-o-
xo-2-pyrazinyl]-2-methylphenyl]-4-(1,1-dimethylethyl)- (CGI-1746CAS
Registry No. 910232-84-7); HM-71224; 2-Propenamide,
N-[3-[[5-fluoro-2-[[4-(2-methoxyethoxy)
phenyl]amino]-4-pyrimidinyl]amino]phenyl]- (CC-292, CAS Registry
No. 1202757-89-8); 2-Pyridinecarboxamide,
4-[4-[[5-fluoro-4-[[3-[(1-oxo-2-propen-1-yl)amino]phenyl]amino]-2-pyrimid-
inyl]amino]phenoxy]-N-methyl- (CNX-774, CAS Registry No.
1202759-32-7), AVL-101 (CAS Registry No. 1552307-34-2), AVL-291
(CAS Registry No. 1552307-35-3), and AVL-292 (CAS Registry No.
1552307-36-4),
[N-(2-chloro-6-methylphenyl)-2-(6-(4-(2-hydroxyethyl)
piperazin-1-yl)-2-methylpyrimidin-4-ylamino)thiazole-5-carboxamide](dasat-
inib), alpha-cyano-beta-hydroxy-beta-methyl-N-(2,5-bromophenyl)
propenamide (LFM-A13), and ONO-WG-307.
##STR00001## ##STR00002##
[0043] Inhibitors of osteoclast activity may be used for a number
of medical purposes, such as treatment of undesirable conditions or
diseases, including pain relief. This may be accomplished in many
instances by administration of oral dosage forms. Generally, an
oral dosage form comprising a bisphosphonate such as zoledronic
acid is administered orally to a mammal, such as a human being, at
least once, to treat a disease or condition, or to relieve
pain.
[0044] The compounds containing Ion 1 or Ion 2 may also be
osteoclast inhibitors:
##STR00003##
[0045] The term "treating" or "treatment" broadly includes any kind
of treatment activity, including the diagnosis, cure, mitigation,
or prevention of disease in man or other animals, or any activity
that otherwise affects the structure or any function of the body of
man or other animals.
[0046] An oral dosage form of a bisphosphonate such as zoledronic
acid may be used to treat, or provide relief of, any type of pain
including, but not limited to, inflammatory pain, arthritis pain,
complex regional pain syndrome, lumbosacral pain, musculoskeletal
pain, neuropathic pain, chronic pain, cancer-related pain, acute
pain, postoperative pain, etc. In some instances, pain relief may
be palliative, or pain relief may be provided independent of
improvement of the disease or condition or the underlying cause of
the disease or condition. For example, although the underlying
disease may not improve, or may continue to progress, an individual
suffering from the disease may experience pain relief. In some
embodiments, enhanced bioavailability of the zoledronic acid may be
achieved in treating one of these conditions by administering a
dosage form comprising zoledronic acid in the form of a disodium
salt. This may allow a reduced molar amount of the disodium salt to
be used as compared to what would be used with the diacid form.
[0047] In some embodiments, the mammal being treated is not
suffering from bone metastasis. In some embodiments, the mammal
being treated is not suffering from cancer. In some embodiments,
the mammal being treated is not suffering from osteoporosis.
[0048] For example, zoledronic acid or another bisphosphonate may
be administered orally to relieve musculoskeletal pain including
low back pain, and pain associated with rheumatoid arthritis,
juvenile rheumatoid arthritis, osteoarthritis, erosive
osteoarthritis, sero-negative (non-rheumatoid) arthropathies,
non-articular rheumatism, peri-articular disorders, axial
spondyloarthritis including ankylosing spondylitis, Paget's
disease, fibrous dysplasia, SAPHO syndrome, transient
osteoarthritis of the hip, vertebral crush fractures, osteoporosis,
etc. In some embodiments, enhanced bioavailability of the
zoledronic acid may be achieved in treating one of these conditions
by administering a dosage form comprising zoledronic acid in the
form of a disodium salt. This may allow a reduced molar amount of
the disodium salt to be used as compared to what would be used with
the diacid form.
[0049] An osteoclast inhibitor, such as a bisphosphonate, e.g.
zoledronic acid, may also be used to treat bone fractures or to
enhance the healing of bone fractures. In some embodiments, a human
being that is treated for CRPS, suffered from a precipitating
injury such as a bone fracture associated with the CRPS at least 4
weeks, at least 8 weeks, at least 12 weeks, at least six months, or
at least 1 year before first administering an osteclast inhibitor,
such as a bisphosphonate, including zoledronic acid, neridronic
acid, etc. Examples of a precipitating event include a fracture, a
cutting injury, a scratch, a puncture injury, etc.
[0050] In some embodiments, zoledronic acid or another
bisphosphonate may also be administered orally to relieve
neuropathic pain, including diabetic peripheral neuropathy,
post-herpetic neuralgia, trigeminal neuralgia, monoradiculopathies,
phantom limb pain, and central pain. Other causes of neuropathic
pain include cancer-related pain, lumbar nerve root compression,
spinal cord injury, post-stroke pain, central multiple sclerosis
pain, HIV-associated neuropathy, and radio-therapy or chemo-therapy
associated neuropathy. In some embodiments, enhanced
bioavailability of the zoledronic acid may be achieved in treating
one of these conditions by administering a dosage form comprising
zoledronic acid in the form of a disodium salt. This may allow a
reduced molar amount of the disodium salt to be used as compared to
what would be used with the diacid form.
[0051] In some embodiments, zoledronic acid or another
bisphosphonate may be administered orally to relieve inflammatory
pain including musculoskeletal pain, arthritis pain, and complex
regional pain syndrome. In some embodiments, enhanced
bioavailability of the zoledronic acid may be achieved in treating
one of these conditions by administering a dosage form comprising
zoledronic acid in the form of a disodium salt. This may allow a
reduced molar amount of the disodium salt to be used as compared to
what would be used with the diacid form.
[0052] Examples of musculoskeletal pain include low back pain; and
pain associated with vertebral crush fractures, fibrous dysplasia,
osteogenesis imperfecta, Paget's disease of bone, transient
osteoporosis, and transient osteoporosis of the hip.
[0053] Arthritis refers to inflammatory joint diseases that can be
associated with pain. Examples of arthritis pain include pain
associated with osteoarthritis, erosive osteoarthritis, rheumatoid
arthritis, juvenile rheumatoid arthritis, sero-negative
(non-rheumatoid) arthropathies, non-articular rheumatism,
peri-articular disorders, neuropathic arthropathies including
Charcot's foot, axial spondyloarthritis including ankylosing
spondylitis, and SAPHO syndrome.
[0054] In some embodiments, a human being that is treated for a
disease or condition, such as an inflammatory condition, e.g.
arthritis or CRPS, by an osteoclast inhibitor, such as a
bisphosphonate, e.g. an oral dosage form of zoledronic acid, has an
age of at least 18 years, at least 50 years (including a male of at
least 50 years), a postmenopausal female, about 10 years to about
90 years, about 20 years to about 80 years, about 30 years to about
75 years, about 40 years to about 70 years, about 1 year to about
16 years, or about 80 years to about 95 years. In some embodiments,
the human being is a male at least 50 years of age or a
postmenopausal female, with knee osteoarthritis (OA) and bone
marrow lessions (BMLs), having moderate or worse knee pain.
[0055] In some embodiments, a human being that is treated for a
disease or condition, such as an inflammatory condition, e.g.
arthritis, low back pain, or CRPS, by an osteoclast inhibitor, such
as a bisphosphonate, e.g. an oral dosage form of zoledronic acid,
has suffered from the inflammatory condition for at least 1 month,
at least 2 months, at least 3 months, at least 6 months, or at
least 1 year.
[0056] In some embodiments, the arthritis affects a knee, an elbow,
a finger, a wrist, a shoulder, an ankle, the spine, or a hip.
[0057] For treatment of arthritis or joint pain, such as knee pain,
in some embodiments the person being treated has OARSI Grade 0, or
Kellgren and Lawrence Grades 0 or 1, joint space narrowing.
[0058] In some embodiments, the person has lesions, such as bone
marrow lesions. In some embodiments the person being treated for
bone marrow lesions has normal joint space knee pain, OARSI Grade
0, or Kellgren and Lawrence Grades 0 or 1, joint space
narrowing.
[0059] In some embodiments, the person has baseline pain intensity
of 5 or greater measured using the 0-10 numerical rating scale
(NRS), or 50 mm or greater using the 100 mm visual analog scale
(VAS). In some embodiments the person being treated for pain has
normal joint space knee pain, OARSI Grade 0, or Kellgren and
Lawrence Grades 0 or 1, joint space narrowing.
[0060] Bone marrow lesions (BMLs) include regional bone marrow
signal intensity alterations on magnetic resonance imaging (MRI).
BMLs can be present in the knee and can be an important feature of
osteoarthritis of the knee. BMLs have also been described in other
rheumatic conditions such as rheumatoid arthritis, osteonecrosis,
ankylosing spondylitis, and transient osteoporosis of the hip and
are often referred to as bone marrow edema (BME).
[0061] In some embodiments, a person being treated for arthritis,
such as with zoledronic acid, has osteoarthritis of the knee
associated with bone marrow lesions.
[0062] In some embodiments, an inhibitor of osteoclast activity can
be used to treat bone marrow lesions.
[0063] In some embodiments, an inhibitor of osteoclast activity can
be used to treat bone marrow lesions of the knee, shoulder, ankle,
wrist, hand, fingers, spine, or hip.
[0064] Commonly used measures of pain intensity include the visual
analog scale (VAS) and the numerical rating scale (NRS). With the
VAS approach, patients rate the severity of their pain by marking a
point on a 10-cm (or 100 mm) VAS (0=no pain and 10=worst possible
pain). With the NRS approach, patients rate the severity of their
pain by verbally responding to a 10-point NRS (0=no pain and
10=worst possible pain). VAS and NRS scores have been shown to be
strongly correlated (slope of regression line, 1.01), indicating
that a score on the 10-cm VAS is equivalent to the same score on
10-point NRS (Bijur P E et al. Acad Emerg Med 2003; 10:390-392).
For example, a VAS score of 5 cm (or 50 mm) is equivalent to an NRS
score of 5. Knee pain in a person with a VAS score of 5 cm or 50 mm
or higher, or an NRS score of 5 or higher, may be referred to
herein as moderate to severe knee pain.
[0065] In some embodiments, the patient suffering from pain,
inflammation, a similar condition, or any of the conditions
described herein, has an NRS of 5 or greater, or a VAS of 5 cm or
greater. In some embodiments, the patient has an NRS of 4 or
greater, or a VAS of 4 cm or greater. In some embodiments, the
patient has an NRS of 6 or greater, or a VAS of 6 cm or greater. In
some embodiments, the patient has an NRS of 7 or greater, or a VAS
of 7 cm or greater. In some embodiments, the patient has an NRS of
about 1, about 2, about 3, about 4, about 5, about 6, about 7,
about 8, about 9, or about 10. In some embodiments, the patient has
a VAS of about 1 cm, about 2 cm, about 3 cm, about 4 cm, about 5
cm, about 6 cm, about 7 cm, about 8 cm, about 9 cm, or about 10
cm.
[0066] For knee pain or pain associated with bone marrow lesions,
in some embodiments, treatment with a nitrogen-containing
bisphosphonate such as zoledronic acid may decrease the visual
analog (VAS) pain score measured using a 100 mm scale, by at least
about 5 mm, at least about 8 mm, at least about 10 mm, at least
about 15 mm, up to about 50 mm, or up to about 100 mm. In some
embodiments, the VAS score, may be decreased by at least about 5
mm, at least about 8 mm, at least about 10 mm, at least about 15
mm, up to about 50 mm, or up to about 100 mm, as compared to a
placebo.
[0067] Treatment with a nitrogen-containing bisphosphonate such as
zoledronic acid may decrease the numerical rating scale (NRS) pain
score measured using a 0-10 scale, by at least about 0.1, at least
about 0.5, at least about 0.8, at least about 1, at least about
1.5, up to about 5, or up to about 10. In some embodiments, the NRS
score may be decreased by at least about 0.1, at least about 0.5,
at least about 0.8, at least about 1, at least about 1.5, up to
about 5, or up to about 10, as compared to a placebo.
[0068] In some embodiments, an inhibitor of osteoclast activity can
be used to reduce the size of bone marrow lesions. The area of the
lesions may be measured as the total area of all lesions or as the
area of any one lesion. In some embodiments, the total area
includes the medial tibial area, the medial femoral area, the
lateral tibial area, and the lateral femoral area. In some
embodiments the bone marrow lesion in located in the patella.
[0069] In some embodiments, the use of an inhibitor of osteoclast
activity achieves a reduction in the total area of the bone marrow
lesions of at least about 240 mm.sup.2. In some embodiments, the
reduction in total area is at least about 220 mm.sup.2, at least
about 200 mm.sup.2, at least about 150 mm.sup.2, at least about 100
mm.sup.2, or at least about 50 mm.sup.2. In some embodiments, the
reduction in size of bone marrow lesions represents a reduction
relative to baseline of at least about 10%, at least about 20%, at
least about 30%, at least about 40%, at least about 50%, at least
about 60%, at least about 70% at least about 80%, at least about
90%, or about 100%. In some embodiments, the reduction in area of
bone marrow lesions represents an improvement relative to placebo
of at least about 10%, at least about 20%, at least about 30%, at
least about 40%, at least about 50%, at least about 60%, at least
about 70%, at least about 80%, at least about 90%, at least about
100%, at least about 120%, at least about 150%, at least about
170%, at least about 200%, at least about 250%, at least about
300%, at least about 350%, at least about 400%, or at least about
450%. In some embodiments, the use of an inhibitor of osteoclast
activity inhibits an increase in the size of the bone marrow
lesions over time.
[0070] Joint space narrowing (JSN) is typically graded using the
Osteoarthritis Research Society International (OARSI) atlas
criteria, or the Kellgren and Lawrence (K/L) system. The OARSI
atlas criteria grades JSN using a 0-3 scale with Grade 0 indicating
an absence of JSN, and Grades 1, 2 and 3 indicating mild, moderate,
and severe JSN, respectively (Altman and Gold, Osteoarthritis
Cartilage 2007; 15(Suppl A):A1-A56). The K/L system grades JSN
using a 0-4 scale with Grade 0 indicating an absence of JSN, Grade
1 indicating doubtful JSN, and grades 2, 3 and 4 indicating
minimal, moderate, and severe JSN, respectively (Kellgren and
Lawrence, Ann Rheum Dis 1957; 16:494-502). Based on these criteria,
OARSI Grade 0 (absence of JSN), approximates K/L Grades 0-1
(absence of, or doubtful presence of JSN). Knee pain in a person
having OARSI Grade 0 or K/L Grade or 1 JSN in the knee where the
pain occurs may be referred to herein as a "normal joint space knee
pain."
[0071] In some embodiments for patients having OARSI Grade 0 or K/L
Grades 0-1 JSN, the use of an inhibitor of osteoclast activity
achieves a reduction in the total area of the bone marrow lesions
of at least about 240 mm.sup.2. In some embodiments, the reduction
in total area is at least about 220 mm.sup.2, at least about 200
mm.sup.2, at least about 150 mm.sup.2, at least about 100 mm.sup.2,
or at least about 50 mm.sup.2. In some embodiments, the reduction
in size of bone marrow lesions represents a reduction relative to
baseline of at least about 10%, at least about 15%, at least about
20%, at least about 25%, at least about 30%, at least about 35%, at
least about 40%, at least about 45%, at least about 50%, at least
about 60%, at least about 70% at least about 80%, at least about
90%, or about 100%. In some embodiments, the reduction in area of
bone marrow lesions represents an improvement relative to placebo
of at least about 10%, at least about 20%, at least about 30%, at
least about 40%, at least about 50%, at least about 60%, at least
about 70%, at least about 80%, at least about 90%, at least about
100%, at least about 120%, at least about 150%, at least about
170%, at least about 200%, at least about 250%, at least about
300%, at least about 350%, at least about 400%, or at least about
450%. In some embodiments, the use of an inhibitor of osteoclast
activity inhibits an increase in the size of bone marrow lesions
over time.
[0072] In some embodiments for patients having OARSI Grades 1-2 or
K/L Grades 2-4 JSN, the use of an inhibitor of osteoclast activity
achieves a reduction in the total area of the bone marrow lesions
of at least about 100 mm.sup.2. In some embodiments, the reduction
in total area is at least about 50 mm.sup.2, at least about 60
mm.sup.2, at least about 80 mm.sup.2, at least about 85 mm.sup.2,
at least about 90 mm.sup.2, at least about 100 mm.sup.2, at least
about 105 mm.sup.2, at least about 110 mm.sup.2, or at least about
115 mm.sup.2. In some embodiments, the reduction in size of bone
marrow lesions represents a reduction relative to baseline of at
least about 10%, at least about 20%, at least about 30%, at least
about 40%, at least about 50%, at least about 60%, at least about
70% at least about 80%, at least about 90%, or about 100%. In some
embodiments, the reduction in area of bone marrow lesions
represents an improvement relative to placebo of at least about
10%, at least about 20%, at least about 30%, at least about 40%, at
least about 50%, at least about 60%, at least about 70%, at least
about 80%, at least about 90%, at least about 100%, at least about
115%, at least about 125%, at least about 135%, at least about
150%, at least about 170%, at least about 200%, at least about
250%, at least about 300%, at least about 350%, at least about
400%, or at least about 450%. In some embodiments, the use of an
inhibitor of osteoclast activity inhibits an increase in the size
of bone marrow lesions over time.
[0073] In some embodiments, an inhibitor of osteoclast activity,
such as a nitrogen-containing bisphosphonate, including e.g.
zoledronic acid, minodronic acid, etc., is used to treat
fibromyalgia.
[0074] According to some embodiments, administration of an
inhibitor of osteoclast activity achieves a reduction in pain that
lasts at least about one month, two months, three months, four
months, six months, or even at least about twelve months. According
some embodiments, administration of an inhibitor of osteoclast
activity achieves a reduction in pain that is observed at greater
than three hours, at about one day, at about two to about five
days, at about one week, at about two weeks, at about three weeks,
at about one month, at about five weeks, at about six weeks, at
about seven weeks, at about two months, at about nine weeks, at
about ten weeks, at about eleven weeks, at about three months, at
about four months, at about six months, or at about twelve months
after administration of the inhibitor of osteoclast activity.
[0075] According some embodiments, administration of an inhibitor
of osteoclast activity achieves a reduction in pain that is
observed at greater than three hours, but at or before one week,
two weeks, three weeks, four weeks, five weeks, six weeks, seven
weeks, eight weeks, nine weeks, ten weeks, eleven weeks, twelve
weeks, four months, five months, or six months.
[0076] According some embodiments, administration of an inhibitor
of osteoclast activity achieves a reduction in pain that is
observed at greater than three hours with a duration of no more
than about three months, no more than about four months, no more
than about five months, or no more than about six months.
[0077] According to some embodiments, after the administration of
an inhibitor of osteoclast activity, the area of bone marrow
lesions relative to the size prior to administration remains
reduced for up to three months, four months, five months, six
months, or even up to twelve months or more. According to some
embodiments, after the administration of an inhibitor of osteoclast
activity, the area of bone marrow lesions relative to the size
prior to administration is reduced at about three months, at about
four months, at about five months, at about six months, or at about
twelve months.
[0078] According to some embodiments, after administration of an
inhibitor of osteoclast activity, the size of Modic changes or
VESCs relative to the size prior to administration remains reduced
for up to three months, four months, five months, six months, or
even up to twelve months or more. According to some embodiments,
after the administration of an inhibitor of osteoclast activity,
the size of Modic changes or VESCs relative to the size prior to
administration is reduced at about three months, at about four
months, at about five months, at about six months, or at about
twelve months.
[0079] In some embodiments, an osteoclast inhibitor, such as a
nitrogen-containing bisphosphonate, e.g. zoledronic acid,
ibandronic acid or minodronic acid, may be administered to relieve
complex regional pain syndrome, such as complex regional pain
syndrome type I (CRPS-I), complex regional pain syndrome type II
(CRPS-II), CRPS-NOS, or another type of CRPS.
[0080] In some embodiments, zoledronic acid or another
bisphosphonate may be administered orally to relieve complex
regional pain syndrome, such as complex regional pain syndrome type
I (CRPS-I), complex regional pain syndrome type II (CRPS-II),
CRPS-NOS, or another type of CRPS. CRPS is a type of inflammatory
pain. CRPS can also have a neuropathic component.
[0081] Complex regional pain syndrome is a debilitating pain
syndrome. It is characterized by severe pain in a limb that can be
accompanied by edema, and autonomic, motor and sensory changes.
[0082] In some embodiments, an osteoclast inhibitor, such as a
nitrogen-containing bisphosphonate, e.g. zoledronic acid or
minodronic acid, may be used to reduce the use of non-steroidal
anti-inflammatory drug (NSAIDs), opioids, or other pain
medications, for a patient suffering from pain, inflammation, a
similar condition, or any condition described herein. For example,
use of NSAIDs, opioids, or other pain medications may be reduced by
at least about 5%, at least about 10%, at least about 15%, at least
about 20%, at least about 25%, at least about 30%, at least about
35%, at least about 40%, at least about 45%, at least about 50%, at
least about 60%, at least about 70%, at least about 80%, or at
least about 90%, up to about 100%, as compared to the use of
NSAIDs, opioids or other pain medications without administration of
the osteoclast inhibitor. Use of the opioids, NSAIDs, or other pain
medications may be reduced by at least about 5%, at least about
10%, at least about 15%, at least about 20%, at least about 25%, at
least about 30%, at least about 35%, at least about 40%, at least
about 45%, at least about 50%, at least about 60%, at least about
70%, at least about 80%, or at least about 90%, up to about 100%,
as compared to the use of NSAIDS, opioids, or other pain
medications at baseline.
[0083] The reduction in the use of NSAIDs, opioids, or other pain
medications may be observed at about one week, about two weeks,
about three weeks, about one month, about two months, about three
months, about four months, about five months, about six months,
about seven months, about eight months, about nine months, about 10
months, about 11 months, or about one year or more, after the
administration of osteoclast inhibitor.
[0084] With respect to use of oral zoledronic acid in a disodium
salt form or in an acid form for relieving pain associated with an
inflammatory condition or Paget's disease of bone, relief of pain
can be short-term, e.g. for a period of hours after administration
of the dosage form, and/or relief of pain can be long-term, e.g.
lasting for days, weeks, or even months after oral administration
of zoledronic acid. In some embodiments, a mammal, such as a human
being, experiences significant pain relief at least about 3 hours,
at least about 6 hours, at least about 12 hours, at least about 24
hours, at least about 48 hours, at least about one week, at least
about 2 weeks, or at least about 3 weeks after administration of an
oral dosage form comprising zoledronic acid. In some embodiments, a
mammal, such as a human being, experiences significant pain relief
during at least part of the time from about 3 hours to about 2
weeks, about 3 hours to about 3 weeks, about 3 hours to about 24
hours, about 6 hours to about 2 weeks, or about 6 hours to about 24
hours, about 3 days to about 2 weeks, about 6 days to about 2
weeks, after administration of an oral dosage form comprising
zoledronic acid. In some embodiments, a human being treated has
significant pain relief at one month, three months, six months,
nine months, one year, 5 years, or longer, after administration of
the most recent dose of an osteoclast inhibitor such as zoledronic
acid.
[0085] With respect to the treatment of any condition recited
herein, in some embodiments a first oral dosage form comprising
zoledronic acid is administered and a second oral dosage form
comprising oral zoledronic acid is administered. The timing of the
administration of the two dosage forms may be such that, with
respect to the first oral dosage form, the second oral dosage with
respect to the first oral dosage form, the second oral dosage form
is administered at 5.times.T.sub.max or greater (e.g., if T.sub.max
is 1 hour, at 5 hours or later), at least 10.times.T.sub.max or
greater, at least about 15.times.T.sub.max or greater, at least
about 20.times.T.sub.max or greater, at least about
50.times.T.sub.max or greater, or at least about
200.times.T.sub.max or greater, wherein T.sub.max is the time of
maximum plasma concentration for the first oral dosage form.
[0086] Some embodiments include treatment of a condition recited
herein, such as inflammatory pain, arthritis, or complex regional
pain syndrome, wherein the treatment comprises either:
administering only one dosage form to a mammal to treat the
condition, or administering a first dosage form to the mammal,
followed by administering a second dosage form to the mammal. If
two or more dosage forms are administered, the second oral dosage
form is administered before the maximum pain relieving effect of
the first oral dosage form is achieved, or before a peak in the
pain relieving effect of the first oral dosage form is experienced
by a mammal, receiving the dosage form. In some embodiments, the
second oral dosage form is administered before an observable pain
relieving effect is achieved. In some embodiments, the second
dosage form is administered about 12 hours to about 60 days, about
24 hours to about 28 days, about 24 hours to about 7 days, about 24
hours to about 14 days, or about 24 hours to about 21 days, after
the first dosage form is administered.
[0087] Some embodiments include treatment of a condition recited
herein, such as inflammatory pain, arthritis, or complex regional
pain syndrome, wherein the treatment comprises administering a
first dosage form to the mammal, followed by administering a second
dosage form to the mammal, wherein the second dosage form is
administered after the maximum pain relieving effect of the first
oral dosage form is achieved, and the second oral dosage form is
administered while the mammal is still experiencing pain relief
from the first oral dosage form, or while the pain relieving effect
from the first oral dosage form is observable. In some embodiments,
the second dosage form is administered about 12 hours to about 60
days, about 24 hours to about 28 days, about 24 hours to about 7
days, about 24 hours to about 14 days, or about 24 hours to about
21 days, after the first dosage form is administered.
[0088] Zoledronic acid or another bisphosphonate may also be
administered orally to relieve cancer-related pain, including pain
associated with multiple myeloma and bone metastases from solid
tumors. In some embodiments, zoledronic acid is used to treat pain
that is not cancer-related pain. For example, zoledronic acid may
be used to treat pain that is not associated with multiple myeloma,
bone metastasis from solid tumors, hypercalcemia of malignancy,
giant cell tumor of bone, blood cancers or leukemias, or solid
tumors or cancers. In some embodiments, enhanced bioavailability of
the zoledronic acid may be achieved in treating one of these
conditions by administering a dosage form comprising zoledronic
acid in the form of a disodium salt. This may allow a reduced molar
amount of the disodium salt to be used as compared to what would be
used with the diacid form.
[0089] In addition to relieving pain, oral administration of
zoledronic acid or another bisphosphonate may also be useful to
treat diseases or conditions that may or may not include a pain
component. For example, zoledronic acid or another bisphosphonate
may be useful to treat any of the pain conditions or types of
conditions listed above, including treatment that does not simply
relieve the pain of those conditions, and treatment that is carried
out in such a way that the condition is treated without pain relief
occurring. In addition to any pain relief zoledronic acid or
another bisphosphonate may or may not provide, zoledronic acid or
another bisphosphonate may be used to treat a disease or condition
such as a metabolic disease or condition; an inflammatory disease
or condition, including an inflammatory disease or condition that
is not associated with pain; a cancer disease or condition; a
neurological disease or condition; etc. In some embodiments,
enhanced bioavailability of the zoledronic acid may be achieved in
treating one of these conditions by administering a dosage form
comprising zoledronic acid in the form of a disodium salt. This may
allow a reduced molar amount of the disodium salt to be used as
compared to what would be used with the diacid form.
[0090] In some embodiments, oral administration of zoledronic acid
or another bisphosphonate may also be useful to treat complex
regional pain syndrome, rheumatoid arthritis, osteoarthritis,
erosive osteoarthritis, axial spondyloarthritis including
ankylosing spondylitis, acute vertebral crush fracture, fibrous
dysplasia, SAPHO syndrome, osteoporosis, transient osteoporosis, or
transient osteoporosis of the hip. In some embodiments, enhanced
bioavailability of the zoledronic acid may be achieved in treating
one of these conditions by administering a dosage form comprising
zoledronic acid in the form of a disodium salt. This may allow a
reduced molar amount of the disodium salt to be used as compared to
what would be used with the diacid form.
[0091] In some embodiments, oral administration of zoledronic acid
or another bisphosphonate may also be useful to treat hypercalcemia
of malignancy, multiple myeloma, bone metastases from solid tumors,
Paget's disease of bone, giant cell tumor of bone, blood cancers or
leukemias, or solid tumors or cancers. In some embodiments,
enhanced bioavailability of the zoledronic acid may be achieved in
treating one of these conditions by administering a dosage form
comprising zoledronic acid in the form of a disodium salt. This may
allow a reduced molar amount of the disodium salt to be used as
compared to what would be used with the diacid form.
[0092] Some nitrogen-containing bisphosphonates may be represented
by Formula A:
##STR00004##
[0093] With respect to Formula A, R.sup.1 is F, Cl, Br, H, or OH.
In some embodiments, R.sup.1 is OH.
[0094] With respect to Formula A, R.sup.2 is aminoalkyl, such as
aminoethyl, aminopropyl, aminopentyl, dimethylaminoethyl,
methylpentylaminoethyl, etc; or optionally substituted heterocyclyl
alkyl, such as optionally substituted imidazolylmethyl, optionally
substituted pyridinymethyl, etc. In some embodiments R.sup.2 is
optionally substituted imidazolylalkyl.
[0095] Unless otherwise indicated, when a compound or chemical
structural feature such as heterocyclyl alkyl is referred to as
being "optionally substituted," it includes a feature that has no
substituents (i.e. unsubstituted), or a feature that is
substituted, meaning that the feature has one or more substituents.
The term "substituent" has the broadest meaning known to one of
ordinary skill in the art, and includes a moiety that replaces one
or more hydrogen atoms in a parent compound or structural feature.
The term "replaces" is merely used herein for convenience, and does
not require that the compound be formed by replacing one atom with
another. In some embodiments, a substituent may be any ordinary
organic moiety known in the art, which may have a molecular weight
(e.g. the sum of the atomic masses of the atoms of the substituent)
of 15 g/mol to 50 g/mol, 15 g/mol to 100 g/mol, 15 g/mol to 150
g/mol, 15 g/mol to 200 g/mol, 15 g/mol to 300 g/mol, or 15 g/mol to
500 g/mol. In some embodiments, a substituent comprises, or
consists of: 0-30, 0-20, 0-10, or 0-5 carbon atoms; and 0-30, 0-20,
0-10, or 0-5 heteroatoms, wherein each heteroatom may independently
be: N, O, P, S, Si, F, Cl, Br, or I; provided that the substituent
includes one C, N, O, P, S, Si, F, Cl, Br, or I atom. In some
embodiments, substituents can independently have a molecular weight
of about 15 Da to about 600 Da and can consist of 2 to 5 chemical
elements, wherein the chemical elements are independently C, H, O,
N, P, S, Si, F, CI, or Br. In some embodiments, a substituent is
optionally substituted alkyl, --O-alkyl (e.g. --OCH.sub.3,
--OC.sub.2H5, --OC.sub.3H.sub.7, --OC.sub.4H.sub.9, etc.),
--S-alkyl (e.g. --SCH.sub.3, --SC.sub.2H5, --SC.sub.3H.sub.7,
--SC.sub.4H.sub.9, etc.), --NR'R'', --OH, --SH, --CN, --CF.sub.3,
--NO.sub.2, perfluoroalkyl, optionally substituted aryl, optionally
substituted heteroaryl, optionally substituted amine or a halogen,
wherein R' and R'' are independently H or optionally substituted
alkyl. Wherever a substituent is described as "optionally
substituted," that substituent can be substituted with the above
substituents.
[0096] For convenience, the term "molecular weight" is used with
respect to a moiety or part of a molecule to indicate the sum of
the atomic masses of the atoms in the moiety or part of a molecule,
even though it may not be a complete molecule.
[0097] Examples of nitrogen-containing bisphosphonates include but
are not limited to pamidronic acid, incadronic acid, ibandronic
acid, risedronic acid, minodronic acid, cimadronic acid, neridronic
acid, alendronic acid, olpadronic acid, zoledronic acid, etc.
##STR00005##
[0098] Zoledronic acid has the structure shown below, and is also
referred to as zoledronate.
##STR00006##
[0099] Unless otherwise indicated, any reference to a compound
herein, such as zoledronic acid, by structure, name, or any other
means, includes pharmaceutically acceptable salts, such as the
disodium salt; alternate solid forms, such as polymorphs, solvates,
hydrates, etc.; tautomers; or any other chemical species that may
rapidly convert to a compound described herein under conditions in
which the compounds are used as described herein. Unless otherwise
indicated, a phrase such as "administering a bisphosphonate,"
"administering an osteoclast inhibitor," "administering zoledronic
acid," includes administering any form of the bisphosphonate,
osteoclast inhibitor, zoledronic acid, etc., such as those recited
above.
[0100] In some embodiments, zoledronic acid is administered in a
dosage form comprising a salt form, such as a salt of a dianion of
zoledronic acid. In some embodiments, zoledronic acid is
administered in a dosage form comprising a disodium salt form of
zoledronic acid. In some embodiments, zoledronic acid is
administered in a sodium salt form, such as a monosodium salt, a
disodium salt, a trisodium salt, etc. In some circumstances, use of
the disodium salt may be desirable. For example, the disodium salt
is much more soluble in water than the diacid form. As a result, in
some processes, the disodium salt can be easier to work with than
the diacid form. Additionally, the sodium salt may be more
bioavailable and/or more rapidly absorbed when taken orally as
compared to the diacid form.
[0101] In some embodiments, a RANK/RANKL antagonists or an
osteoclast inhibitor, such as zoledronic acid or neridronic acid
may be in the form of a molecular complex. For example, molecular
complexes of zoledronic acid include cocrystals, salts, solvates
such as hydrates and mixed solvates of an acid or a salt form, and
mixtures containing such materials. Molecular complexes of
zoledronic acid may be in amorphous forms or polymorphs.
[0102] Of particular interest are compositions, or complexes
comprising zoledronic acid or neridronic acid and the standard
amino acids or natural existing amino acids, such as alanine,
arginine, asparagine, aspartic acid, cysteine, glutamic acid,
glutamine, glycine, histidine, isoleucine, leucine, lysine,
methionine, phenylalanine, proline, serine, threonine, tryptophan,
tyrosine, valine, etc. Some examples of useful molecular complexes
include, but are not limited to, complexes of zoledronic acid or
neridronic acid with sodium cation, ammonium, ammonia, L-lysine,
DL-lysine, nicotinamide, adenine, glycine, and Selenocysteine.
[0103] Zoledronic acid may also be in a form represented by one of
the structural depictions below.
##STR00007##
[0104] Zoledronic acid in a salt or an acid form may be present in
a molecular complex having strong X-ray powder diffraction peaks in
one of the following positions:
TABLE-US-00001 strong X-ray powder diffraction peaks Form
(.degree.2.theta. .+-. 0.2) zoledronic acid, sodium zoledronate and
about 8.1, about 13.3, about 21.5, about water complex 24.6, and
about 25.6 ammonium zoledronate salt and water about 11.0, about
14.6, about 15.4, complex about 19.9, and about 29.4 zoledronic
acid, L-lysine, and water about 9.0, about 14.4, about 18.1, about
complex 26.0, and about 29.6 zoledronic acid, DL-lysine, and water
about 9.1, about 14.7, about 18.0, about complex 21.2, and about
26.0 zoledronic acid, DL-lysine, ethanol, and about 8.8, about 9.7,
about 17.6, about water complex 23.1, and about 26.5 zoledronic
acid, nicotinamide, and water 13.1, about 15.2, about 21.0, about
23.9, complex and about 26.5 zoledronic acid, adenine, and water
about 13.6, about 15.9, about 19.7, complex about 27.9, and about
29.5 zoledronic acid and glycine complex about 10.2, about 17.8,
about 19.9, about 22.9, and about 28.1 zoledronic acid diammonia,
and water about 12.2, about 13.0, about 14.1, complex about 17.1,
and about 19.3 zoledronic acid, DL-lysine, and water about 8.3,
about 11.8, about 12.3, about complex 15.8, and about 20.8
zoledronic acid, L-lysine, and water about 9.6, about 10.7, about
14.3, about complex 21.4, and about 23.5 zoledronic acid,
DL-lysine, and water about 9.7, about 10.8, about 14.4, about
complex 18.9, and about 21.4 zoledronic acid, DL-lysine complex
7.2, about 14.0, about 18.3, about 19.1, about 20.7, about 24.6,
and about 34.4 zoledronic acid, DL-lysine complex 6.6, about 11.0,
about 14.2, about 18.3, about 19.7, about 22.7, and about 27.6
[0105] Solid forms of zoledronic acid such as complexes of
zoledronic acid with sodium, ammonium, ammonia, L-lysine,
DL-lysine, nicotinamide, adenine and glycine may be prepared by
methods such as dry or solvent-drop grinding (liquid assisted
grinding), heating or solvent evaporation of their solution in
single or mixed solvent systems, slurry suspension, supercritical
fluids or other techniques known to a person skilled in the
art.
[0106] For example, zoledronic acid and nicotinamide may be
complexed by dissolving both compounds in water:ethyl acetate (1:1
v/v) and allowing the solvents in the mixture to evaporate to form
crystalline material.
[0107] In some embodiments, a zoledronic acid complex may have an
excess at least one coformer (e.g. the component other than
zoledronic acid) to the zoledronic acid complexes, which may be the
same as the coformer in the complex, a different coformer, or a
mixture thereof. In some embodiments, the excess coformer may be a
standard or natural amino acid. Examples of compounds in salt forms
containing Ion 1 are shown below:
##STR00008##
wherein X is any suitable anion, e.g. F.sup.-, Br.sup.-, Cl.sup.-,
I.sup.-, OH.sup.-, acetate, etc.; and M.sup.+ is any suitable
cation, e.g. Na.sup.+, K.sup.+, NH.sub.4.sup.+, etc. Many other
salt forms are also possible.
[0108] In some embodiments, a compound containing Ion 1 may be
further represented by a formula,
##STR00009##
[0109] In some embodiments, a compound containing Ion 1 may be in a
hydrate form.
[0110] In some embodiments, a compound containing Ion 1 is
administered in a dosage form comprising a salt form, such as a
zwitterionic form, or a salt of a cation, a monoanion, a dianion, a
trianion, etc.
[0111] A compound containing Ion 1 can be present in any amount,
such as less than about 100% w/w, less than about 50% w/w, less
than about 20% w/w, less than about 10% w/w, less than about 1%
w/w, less than 0.1% w/w, less than about 0.07% w/w, less than about
0.05% w/w, less than about 0.04% w/w, less than about 0.03% w/w,
less than about 0.02% w/w; and/or greater than 0% w/w, at least
about 0.00000001% w/w, at least about 0.000001% w/w, or at least
about 0.00001% w/w, based upon the total amount of zoledronic acid,
a compound containing Ion 1, and a compound containing Ion 2
present in the composition.
[0112] Examples of salts of compounds containing Ion 2 are shown
below:
##STR00010##
wherein X.sup.- is any suitable anion, e.g. F.sup.-, Br.sup.-,
Cl.sup.-, I.sup.-, OH.sup.-, acetate, etc.; and M.sup.+ is any
suitable cation, e.g. Na.sup.+, K.sup.+, NH.sub.4.sup.+, etc. Many
other salt forms are also possible.
[0113] In some embodiments, a salt of a compound containing Ion 2
may be further represented by a formula,
##STR00011##
[0114] In some embodiments, a compound containing Ion 2 may be in a
hydrate form.
[0115] In some embodiments, a compound containing Ion 2 is
administered in a dosage form comprising a salt form, such as a
zwitterionic form, or a salt of a cation, a monoanion, a dianion, a
trianion, etc.
[0116] A compound containing Ion 2 can be present in any amount,
such as less than about 100% w/w, less than about 50% w/w, less
than about 20% w/w, less than about 10% w/w, less than about 1%
w/w, less than about 0.3%, less than about 0.2%, less than 0.1%
w/w, less than about 0.08% w/w, less than about 0.07% w/w, less
than about 0.05% w/w, less than about 0.04% w/w, less than about
0.03% w/w, less than about 0.02% w/w; and/or greater than 0% w/w,
at least about 0.00000001% w/w, at least about 0.000001% w/w, or at
least about 0.00001% w/w, based upon the total amount of zoledronic
acid, a compound containing Ion 1, and a compound containing Ion 2
present in the composition.
[0117] In some embodiments, a compound containing Ion 1 and a
compound containing Ion 2 are present in an amount that is less
than 0.1% w/w.
[0118] In some embodiments, the administration of an osteoclast
inhibitor, such as a nitrogen-containing bisphosphonate, including,
e.g. zoledronic acid, minodronic acid, etc., to a patient or mammal
in need thereof affects Modic changes (MCs). For example, any of
the above compounds could be used to treat Modic changes, or
vertebral endplate signal changes (VESC) and bone marrow changes
visible using magnetic resonance imaging (MRI), or neck pain or
back pain associated with Modic changes.
[0119] Modic changes, as used herein, includes its ordinary meaning
in the art and refers to pathological vertebral endplate and bone
marrow changes visible using magnetic resonance imaging (MRI).
Modic changes may also be referred to as vertebral endplate signal
changes (VESC). Modic changes, can be classified into various types
including type 1 (M1), type 2 (M2), and type 3 (M3) lesions or
changes, any of which may be treated using an osteoclast inhibitor,
such as a nitrogen-bisphosphonate, including, e.g. zoledronic acid,
minodronic acid, etc. Different types of Modic changes may occur in
the same patient, for example type 1 and type 2 Modic changes
(M1/2). In some cases, M1 changes are related to lower back pain
than other types of Modic change.
[0120] VESCs may be found in patients with different types of low
back pain including but not limited to spondylitis, trauma,
spondyloarthropathies including ankylosing spondylitis, Schmorl's
nodes, fracture, tumor, and spinal cord infarction. Lesions in
ankylosing spondylitis include osteitis and spondylodiscitis, which
can be detected using MRI or another medical imaging
instrument.
[0121] Modic changes may be found in the cervical, thoracic,
lumbar, and sacral spine. Modic changes may be found at various
spinal levels such as at C1/2, C2/3, C3/4, C4/5, C5/6, C6/7, C7/T1,
T1/2, T2/3, T3/4, T4/5, T5/6, T6/7, T7/8, T8/9, T9/10, T10/11,
T11/12, T12/L1, L1/2, L2/3, L3/4, L4/5, L5/S1, etc., any of which
may be treated using an osteoclast inhibitor, such as a
nitrogen-bisphosphonate, including, e.g. zoledronic acid,
minodronic acid, etc.
[0122] In some embodiments, the Modic change being treated is
located at L2/3. In some embodiments, the Modic change being
treated is located at L3/4. In some embodiments, the Modic change
being treated is located at L4/5. In some embodiments, the Modic
change being treated is located at L5/S1.
[0123] In some embodiments, the Modic change being treated is
located at C3/4. In some embodiments, the Modic change being
treated is located in at C4/5. In some embodiments, the Modic
change being treated is located in at C5/6. In some embodiments,
the Modic change being treated is located in at C6/7.
[0124] In some embodiments, the Modic change being treated is
located at T5/6. In some embodiments, the Modic change being
treated is located in at T6/7. In some embodiments, the Modic
change being treated is located in at T7/8. In some embodiments,
the Modic change being treated is located in at T8/9. In some
embodiments, the Modic change being treated is located at
T9/10.
[0125] In some embodiments, the patient being treated has
predominantly M1. In some embodiments, the patient being treated
has predominantly M1/M2. In some embodiments, the patient being
treated has predominantly M2. In some embodiments, the patient
being treated has predominantly M3.
[0126] In some embodiments, the worst type of lesion that the
patient being treated has is M1. In some embodiments, the worst
type of lesion that the patient being treated has is M1/2. In some
embodiments, the worst type of lesion that the patient being
treated has is M2.
[0127] In some embodiments, the patient being treated has Modic
changes at more two or more levels. In some embodiments the patient
being treated has Modic changes at three or more levels. In some
embodiments greater pain relief is obtained when treating a patient
with Modic changes at two levels, or three or more levels, than is
obtained when treating a patient with Modic changes at a single
level or at two levels.
[0128] In some embodiments greater pain relief is obtained when
treating a patient with Modic changes at two levels than is
obtained when treating a patient with Modic changes at a single
level.
[0129] In some embodiments greater pain relief is obtained when
treating a patient with Modic changes at three or more levels than
is obtained when treating a patient with Modic changes at a single
level.
[0130] In some embodiments greater pain relief is obtained when
treating a patient with Modic changes three or more levels than is
obtained when treating a patient with Modic changes at two
levels.
[0131] In some embodiments, the inhibitor of osteoclast activity
may be used to effect a reduction in the levels of pro-inflammatory
cytokines in the patient with low back pain or any other type of
pain or condition recited herein. In some embodiments greater pain
relief may be obtained in patients with greater baseline levels of
pro-inflammatory cytokines when treated with an inhibitor of
osteoclast activity, such as a nitrogen-containing bisphosphonate,
including e.g. zoledronic acid, minodronic acid, etc. In some
embodiments, greater pain relief may be obtained in patients who
experience a reduction or a greater reduction in the levels of
pro-inflammatory cytokines when treated with an inhibitor of
osteoclast activity, such as a nitrogen-containing bisphosphonate,
including e.g. zoledronic acid, minodronic acid, etc.
Pro-inflammatory cytokines include but are not limited to IL-1,
IL-2, IL-3, IL-6, IL-8, IL-10, IL-12, tumor necrosis alpha
(TNF-alpha), interferon gamma, etc.
[0132] In some embodiments, the use of an inhibitor of osteoclast
activity, such as a nitrogen-containing bisphosphonate, including
e.g. zoledronic acid, minodronic acid, etc., to a patient or mammal
in need thereof, achieves a reduction relative to baseline in the
size of Modic changes or VESCs of at least about 5%, at least about
10%, at least about 15%, at least about 20%, at least about 25%, at
least about 30%, at least about 40%, at least about 50%, at least
about 60%, at least about 70% at least about 80%, at least about
90%, or about 100%. In some embodiments, the reduction the size of
Modic changes or VESCs represents an improvement relative to
placebo of at least about 10%, at least about 15%, at least about
20%, at least about 25%, at least about 30%, at least about 40%, at
least about 50%, at least about 60%, at least about 70%, at least
about 80%, at least about 90%, at least about 100%, at least about
120%, at least about 150%, at least about 170%, at least about
200%, at least about 250%, at least about 300%, at least about
350%, at least about 400%, or at least about 450%. In some
embodiments, the use of an inhibitor of osteoclast activity
inhibits an increase in the size of Modic changes or VESCs over
time.
[0133] The oral bioavailability of zoledronic acid may be enhanced
by orally administering the zoledronic acid in the disodium salt
form. For example, the bioavailability of zoledronic acid may be
improved by at least about 10%, at least about 20%, at least about
30%, at least about 50%, and/or up to about 100%, or up to about
200%, as compared to administration of zoledronic acid in the
diacid form.
[0134] Because of the improved bioavailability of the disodium salt
a dosage form may contain, or a mammal, such as a human being, may
receive, on a molar basis, less of the disodium salt form of
zoledronic acid than would otherwise be administered of the diacid
form of zoledronic acid. For example, a dosage form may contain, or
a mammal may receive, at least about 10 mole % less, at least about
20 mole % less, at least about 40 mole % less, at least about 50
mole % less, and/or up to about 90 mole % less or 95 mole % less,
of the disodium salt form as compared the amount of the diacid form
of zoledronic acid that would otherwise be administered, such as a
molar amount that would be administered of zoledronic acid in the
diacid form in order to achieve the same plasma levels of
zoledronic acid.
[0135] In some embodiments, a dosage form contains, or a mammal
(such as a human being) is administered, an amount of the disodium
salt form, on a molar basis, that has a value of about 0.8n.sub.d
to about 1.2n.sub.d or about 0.9n.sub.d to about 1.1 n.sub.d,
wherein:
n.sub.d=(b.sub.a/b.sub.d)(n.sub.a)
wherein b.sub.a is the bioavailability of the diacid form, b.sub.d
is the bioavailability of the disodium salt form, and n.sub.a is
the number of moles of the diacid that would be administered in a
dosage form containing the diacid form of zoledronic acid. For
example, if the diacid form has a bioavailability (b.sub.a) of 0.01
and the disodium salt form has a bioavailability (b.sub.d) of
0.015, and a dosage form would normally contain 0.001 moles of the
diacid, n.sub.d would be (0.01/0.015)(0.001 moles), or about
0.00067 moles. In some embodiments, the disodium salt is
administered in an amount that has a value of about n.sub.d.
[0136] With respect to oral dosage forms comprising a reduced molar
amount of the disodium salt of zoledronic acid as compared to the
diacid form of zoledronic acid, in some embodiments, the
bioavailability of the zoledronic acid in the disodium salt form is
sufficiently high that, if the drug is administered to a mammal, at
least as much zoledronic acid is present in the blood of the mammal
as would be present if zoledronic acid were administered in the
diacid form.
[0137] With respect to oral dosage forms comprising the disodium
salt form of zoledronic acid, in some embodiments, the disodium
salt form is present in a lower molar amount than would be present
if the zoledronic acid were in the diacid form; and the zoledronic
acid in the disodium salt form has an improved bioavailability as
compared to the zoledronic acid in the diacid form to the extent
that the lower molar amount of the disodium salt in the dosage form
does not reduce the amount of zoledronic acid delivered to the
plasma of a mammal.
[0138] Some oral dosage forms comprising zoledronic acid have a
dose of zoledronic acid and a configuration suitable for a
particular species of mammal, e.g. dog, rat, human, etc. Such a
dosage form may have zoledronic acid present in an amount that
results in a desired range for an area under the plasma
concentration curve (AUC) of zoledronic acid in that particular
species of mammal. For example the dose of zoledronic acid and a
configuration of the oral dosage form may result in an AUC of
zoledronic acid of about 1 ngh/mL to about 700 ngh/mL, about 3
ngh/mL to about 30 ngh/mL, about 3 ngh/mL to about 10 ngh/mL, about
50 ngh/mL to about 700 ngh/mL, about 130 ngh/mL to about 180
ngh/mL, about 300 ngh/mL to about 450 ngh/mL, about 300 ngh/mL to
about 350 ngh/mL, about 300 ngh/mL to about 310 ngh/mL, about 340
ngh/mL to about 350 ngh/mL, about 370 ngh/mL to about 420 ngh/mL,
about 380 ngh/mL to about 390 ngh/mL, about 405 ngh/mL to about 415
ngh/mL, about 140 ngh/mL to about 160 ngh/mL, about 140 ngh/mL to
about 150 ngh/mL, about 150 ngh/mL to about 160 ngh/mL, about 140
ngh/mL, 142 ngh/mL, about 155 ngh/mL, about 305 ngh/mL, 304 ngh/mL,
about 345 ngh/mL, 343 ngh/mL, about 385 ngh/mL, 384 ngh/mL, about
410 ngh/mL, or any AUC in a range bounded by, or between, any of
these values, upon administration of the oral dosage form to a
mammal.
[0139] Unless otherwise indicated, the AUC refers to the AUC
calculated to the last measured concentration (AUC.sub.(0-t)) and
extrapolated to infinity (AUC.sub.(0-inf)).
[0140] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may have zoledronic acid present in an amount
that results in a C.sub.max of zoledronic acid of about 0.2 ng/mL
to about 300 ng/mL, about 0.5 ng/mL to about 5 ng/mL, about 5 ng/mL
to about 300 ng/mL, about 5 ng/mL to about 50 ng/mL, about 20 ng/mL
to about 50 ng/mL, about 30 ng/mL to about 50 ng/mL, about 50 ng/mL
to about 200 ng/mL, about 50 ng/mL to about 150 ng/mL, about 80
ng/mL to about 120 ng/mL, about 90 ng/mL to about 100 ng/mL, about
50 ng/mL to about 200 ng/mL, about 40 ng/mL, about 95 ng/mL, about
97 ng/mL, or any C.sub.max in a range bounded by, or between, any
of these values, upon administration of the oral dosage form to a
mammal.
[0141] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that administration of the
oral dosage form to the particular species of mammal results in a
T.sub.max of zoledronic acid of about 0.4 hr to about 1 hr, about
0.5 hr, or about 0.75 hr, or any T.sub.max in a range bounded by,
or between, any of these values.
[0142] In some embodiments, the zoledronic acid in the disodium
salt form is present in an amount such that the oral dosage form
provides an area under the plasma concentration curve of zoledronic
acid of about 4 ngh/mL to about 2000 ngh/mL to the mammal each time
the zoledronic acid in the disodium salt is administered.
[0143] In some embodiments, the zoledronic acid, including
zoledronic acid in an acid or a salt form, e.g the disodium salt
form, is present in an amount such that the oral dosage form
provides an area under the plasma concentration curve of zoledronic
acid of about 100 ngh/mL to about 2000 ngh/mL, about 100 ngh/mL to
about 1000 ngh/mL, about 500 ngh/mL to about 1000 ngh/mL, or about
500 ngh/mL to about 700 ngh/mL in the mammal to which the dosage
form is administered. This amount may be suitable for
administration of the oral dosage form about every 3 to 4
weeks.
[0144] In some embodiments, the zoledronic acid, such as zoledronic
acid in an acid form or a salt form, such as the disodium salt
form, is present in an amount such that the oral dosage form
provides an area under the plasma concentration curve (AUC) of
zoledronic acid of about 20 ngh/mL to about 700 ngh/mL, about 50
ngh/mL to about 500 ngh/mL, about 50 ngh/mL to about 400 ngh/mL,
about 50 ngh/mL to about 300 ngh/mL, about 50 ngh/mL to about 200
ngh/mL, about 50 ngh/mL to about 100 ngh/mL, about 130 ngh/mL to
about 150 ngh/mL, about 130 ngh/mL to about 140 ngh/mL, about 150
ngh/mL to about 200 ngh/mL, about 200 ngh/mL to about 300 ngh/mL,
about 250 ngh/mL to about 300 ngh/mL, about 300 ngh/mL to about 400
ngh/mL, about 400 ngh/mL to about 500 ngh/mL, about 350 ngh/mL to
about 400 ngh/mL, about 450 ngh/mL to about 500 ngh/mL, about 130
ngh/mL to about 160 ngh/mL, about 405 ngh/mL to about 450 ngh/mL,
about 100 ngh/mL to about 500 ngh/mL, about 100 ngh/mL to about 400
ngh/mL, about 100 ngh/mL to about 300 ngh/mL, about 100 ngh/mL to
about 200 ngh/mL, about 125 ngh/mL to about 500 ngh/mL, about 125
ngh/mL to about 400 ngh/mL, about 125 ngh/mL to about 300 ngh/mL,
about 125 ngh/mL to about 200 ngh/mL, or about 200 ngh/mL to about
300 ngh/mL, in the mammal to which the dosage form is administered.
This amount may be suitable for weekly administration of the oral
dosage, or for administration of 3 to 5 individual dosages during a
month. The individual dosages could be given at regular intervals,
given during the first week, or at any other schedule that provides
3 to 5 dosages during the month.
[0145] In some embodiments, the zoledronic acid is present in an
amount such that the oral dosage form provides an area under the
plasma concentration curve of zoledronic acid of about 4 ngh/mL to
about 100 ngh/mL, about 10 ngh/mL to about 50 ngh/mL, about 10
ngh/mL to about 30 ngh/mL, 20 ngh/mL to about 700 ngh/mL, about 50
ngh/mL to about 500 ngh/mL, about 50 ngh/mL to about 400 ngh/mL,
about 50 ngh/mL to about 300 ngh/mL, about 50 ngh/mL to about 200
ngh/mL, about 100 ngh/mL to about 500 ngh/mL, about 100 ngh/mL to
about 400 ngh/mL, about 100 ngh/mL to about 300 ngh/mL, about 100
ngh/mL to about 200 ngh/mL, about 125 ngh/mL to about 500 ngh/mL,
about 125 ngh/mL to about 400 ngh/mL, about 125 ngh/mL to about 300
ngh/mL, about 125 ngh/mL to about 200 ngh/mL, or about 200 ngh/mL
to about 300 ngh/mL in the mammal to which the dosage form is
administered. This amount may be suitable for daily administration
of the oral dosage form. In some embodiments, the dosage form may
be administered for 2, 3, 4, 5, 6, 7, 8, 9, or 10, 5 to 10, or 6 to
10 consecutive days.
[0146] In some embodiments, the zoledronic acid, such as zoledronic
acid in an acid form or a salt form, such as the disodium salt
form, is present in an amount such that the oral administration of
the dosage form in a fasted state results in an area under the
plasma concentration curve (AUC) of zoledronic acid of about 50
ngh/mL to about 500 ngh/mL, about 50 ngh/mL to about 100 ngh/mL,
about 100 ngh/mL to about 200 ngh/mL, about 130 ngh/mL to about 180
ngh/mL, about 130 ngh/mL to about 150 ngh/mL, about 130 ngh/mL to
about 140 ngh/mL, about 140 ngh/mL to about 150 ngh/mL, about 150
ngh/mL to about 200 ngh/mL, about 200 ngh/mL to about 300 ngh/mL,
about 250 ngh/mL to about 300 ngh/mL, about 300 ngh/mL to about 400
ngh/mL, about 300 ngh/mL to about 350 ngh/mL, about 400 ngh/mL to
about 500 ngh/mL, about 350 ngh/mL to about 400 ngh/mL, about 450
ngh/mL to about 500 ngh/mL, about 130 ngh/mL to about 160 ngh/mL,
about 405 ngh/mL to about 450 ngh/mL, measured over a 24 hour
period.
[0147] In some embodiments, molecular complex comprising neridronic
acid is administered in an amount that results in an AUC of
neridronic acid, measured over the entire course of treatment, of
about 10,000-30,000 ngh/mL about 30,000-100,000 ngh/mL about
30,000-50,000 ngh/mL, about 30,000-40,000 ngh/mL, about
40,000-50,000 ngh/mL, about 50,000-60,000 ngh/mL, about
60,000-70,000 ngh/mL, about 50,000-70,000 ngh/mL, about
70,000-80,000 ngh/mL, about 80,000-90,000 ngh/mL, about
90,000-100,000 ngh/mL, about 70,000-100,000 ngh/mL, about
100,000-200,0000 ngh/mL, about 200,000-300,0000 ngh/mL, about
300,000-400,0000 ngh/mL, about 400,000-500,0000 ngh/mL, or any AUC
in a range bounded by any of these values.
[0148] In some embodiments, an osteoclast inhibitor, a
bisphosphonate, or a RANK/RANKL antagonist, such as zoledronic
acid, etc., is administered at an interval of about once, twice, or
thrice daily, or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13,
or 14 days; or 15, 16, 17, 18, 19, 20, or 21 days; or 22, 23, 24,
25, 26, 27, 28, 29, 30, or 31 days; or 32, 33, 34, 35, 36, 37, 38,
39, 40, 41, 42, 43, 44, or 45; or 46, 47, 48, 49, 50, 51, 52, 53,
54, 55, 56, 57, 58, 59, or 60 days; or 61, 62, 63, 64, 65, 66, 67,
68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,
85, 86, 87, 88, 89, or 90 days; or 91, 92, 93, 94, 95, 96, 97, 98,
99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,
112, 113, 114, 115, 116, 117, 118, 119, or 120 days.
[0149] Oral administration of zoledronic acid, particularly oral
administration of the disodium salt form of zoledronic acid, can
result in more sustained plasma levels of the drug as compared to
parenteral modes of administration, such intravenous or
subcutaneous. For example, the amount of zoledronic acid in the
plasma can be significantly higher for oral administration of the
disodium salt about 24 hours or 48 hours, or longer, after
administration. In some embodiments, oral zoledronic acid has a 24
hour sustained plasma level factor of about 1 or higher, such as
about 1 to about 10, about 1 to about 5, about 3 to about 5, or
about 3 to about 4. In some embodiments, an orally administered
dosage form of zoledronic acid has a 24 hour sustained plasma level
factor or a 48 hour sustained plasma level factor that is higher,
such as at least 1.2 times, at least about 2 times, at least about
5 times, about 1.2 times to about 20 times, about 2 times to about
15 times, about 5 times to about 10 times, or about 8 to about 15
times that of intravenously administered zoledronic acid. A
"sustained plasma level factor," p.sub.f, is determined by the
equation:
p.sub.f=1000(C.sub.t/C.sub.max)
wherein C.sub.max is the maximum plasma concentration of zoledronic
acid after it is administered and C.sub.t is the plasma
concentration of zoledronic acid at the time of interest, such as
24 hours. For parenteral administration, the C.sub.max can be about
the C.sub.0, or the concentration right after injection of the
entire amount of the drug into the body. Sustained plasma level
factors can also be obtained for other times, such as 48 hours, by
using the plasma concentration of zoledronic acid for C.sub.t in
the equation above. For example, if the maximum plasma level of
zoledronic acid after administration is 1000 ng/mL and the plasma
level of zoledronic acid at 24 hours is 1 ng/mL, the 24 hour
sustained plasma level factor is 1.
[0150] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the zoledronic acid has
a 12 hour sustained plasma level factor of about 12 to about 50,
about 20 to about 40, about 25 to about 30, about 30 to about 35,
about 35 to about 40, about 33, about 30, about 35, or any 12 hour
sustained plasma level factor in a range bounded by, or between,
any of these values, for the particular species of mammal.
[0151] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the zoledronic acid has
a 24 hour sustained plasma level factor of about 10 to about 30,
about 10 to about 20, about 10 to about 15, about 12 to about 15 or
16, about 15 to about 20, about 14, about 12, about 15, or any 24
hour sustained plasma level factor in a range bounded by, or
between, any of these values, for the particular species of
mammal.
[0152] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the zoledronic acid has
a 36 hour sustained plasma level factor of about 6 to about 20,
about 8 to about 15, about 9 to about 12 or 13, about 8 to about
10, about 11 to about 13, about 9, about 13, or any 24 hour
sustained plasma level factor in a range bounded by, or between,
any of these values, for the particular species of mammal.
[0153] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the zoledronic acid has
a 48 hour sustained plasma level factor of about 5 to about 20,
about 6 to about 15, about 7 or 8 to about 12 or 13, about 8 to
about 10, about 11 to about 13, about 8, about 12, or any 48 hour
sustained plasma level factor in a range bounded by, or between,
any of these values, for the particular species of mammal.
[0154] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the zoledronic acid has
a 72 hour sustained plasma level factor of about 4 to about 20,
about 5 to about 10, about 5 or 6 to about 10 or 11, about 5 to
about 6, about 9 to about 10, about 6, about 10, or any 72 hour
sustained plasma level factor in a range bounded by, or between,
any of these values, for the particular species of mammal.
[0155] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the particular species
of mammal has a plasma concentration of zoledronic acid at 12 hours
that is about 0.5 ng/mL to about 5 ng/mL, about 1 ng/mL to about 3
ng/mL, about 1 ng/mL to about 2 ng/mL, about 2 ng/mL to about 3
ng/mL, about 3 ng/mL to about 4 ng/mL, about 1.2 ng/mL, about 2.6
ng/mL, about 3.2 ng/mL, or any plasma concentration in a range
bounded by, or between, any of these values.
[0156] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the particular species
of mammal has a plasma concentration of zoledronic acid at 24 hours
that is about 0.2 ng/mL to about 2 ng/mL, about 0.5 ng/mL to about
1.5 ng/mL, about 0.5 ng/mL to about 1 ng/mL, about 1 ng/mL to about
1.5 ng/mL, about 0.5 ng/mL, about 1.0 ng/mL, about 1.4 ng/mL, or
any plasma concentration in a range bounded by, or between, any of
these values.
[0157] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the particular species
of mammal has a plasma concentration of zoledronic acid at 36 hours
that is about 0.1 ng/mL to about 2 ng/mL, about 0.2 ng/mL to about
1.5 ng/mL, about 0.2 ng/mL to about 0.5 ng/mL, about 0.5 ng/mL to
about 1 ng/mL, about 1 ng/mL to about 1.3 ng/mL, about 0.3 ng/mL,
about 0.8 ng/mL, about 1.1 ng/mL, or any plasma concentration in a
range bounded by, or between, any of these values.
[0158] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the particular species
of mammal has a plasma concentration of zoledronic acid at 48 hours
that is about 0.1 ng/mL to about 2 ng/mL, about 0.2 ng/mL to about
1.5 ng/mL, about 0.2 ng/mL to about 0.5 ng/mL, about 0.5 ng/mL to
about 0.9 ng/mL, about 0.9 ng/mL to about 1.3 ng/mL, about 0.3
ng/mL, about 0.7 ng/mL, about 1.1 ng/mL, or any plasma
concentration in a range bounded by, or between, any of these
values.
[0159] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the particular species
of mammal has a plasma concentration of zoledronic acid at 72 hours
that is about 0.2 ng/mL to about 1 ng/mL, about 0.2 ng/mL to about
1.5 ng/mL, about 0.1 ng/mL to about 0.3 ng/mL, about 0.3 ng/mL to
about 0.6 ng/mL, about 0.6 ng/mL to about 1 ng/mL, about 0.2 ng/mL,
about 0.5 ng/mL, about 0.9 ng/mL, or any plasma concentration in a
range bounded by, or between, any of these values.
[0160] An oral dosage form comprising zoledronic acid having a dose
of zoledronic acid and a configuration suitable for a particular
species of mammal may be configured so that the elimination
half-life of zoledronic acid in the particular species of mammal is
about 30 hours to about 100 hours, about 40 hours to about 60
hours, about 40 hours to about 50 hours, about 50 hours to about 60
hours, about 42 hours, about 51 hours, about 59 hours, or any
half-life in a range bounded by, or between, any of these
values.
[0161] As used herein, the "elimination half-life" refers to the
apparent first-order terminal plasma elimination half-life,
obtained by non-compartmental analysis using Win-Nonlin. A terminal
plasma elimination half-life is the time required to reduce the
plasma concentration to half after reaching pseudo-equilibrium, and
not the time required to eliminate half the administered dose. For
orally administered drugs, terminal plasma elimination half-life
can be affected by absorption of the drug, as well as plasma
clearance and extent of distribution.
[0162] In some embodiments, the disodium salt form of zoledronic
acid provides an enhancement to bioavailability, as compared to the
diacid form of zoledronic acid, which adds to any enhancement to
bioavailability provided by any bioavailability-enhancing agents in
the dosage form. In some embodiments, the disodium salt form of
zoledronic acid provides an enhancement to bioavailability, as
compared to the diacid form of zoledronic acid, which is greater
than any enhancement to bioavailability provided by any
bioavailability-enhancing agents in the dosage form. In some
embodiments, the disodium salt form of zoledronic acid may be
administered in a dosage form that is substantially free of
bioavailability-enhancing agents.
[0163] The C-terminal telopeptide (CTX) is one of the products from
type I collagen degradation by osteoclasts during bone resorption.
Thus, CTX serum levels may be used as a biomarker to indicate and
monitor bone breakdown, resorption, and loss. In some embodiments,
zoledronic acid and other bisphosphonates may be used to inhibit
osteoclast activity and/or lower CTX serum levels, for example, by
at least about 5%, at least about 10%, at least about 15%, at least
about 20%, at least about 25%, at least about 30%, at least about
35%, at least about 40%, at least about 45%, at least about 50%, at
least about 55%, at least about 60%, at least about 70%, at least
about 75%, at least about 80%, at least about 85%, at least about
90%, at least about 95%, at least about 99%, at least about 100%,
about 60%-70%, about 70%-80%, about 80%-90%, about 85-95%, about
80%-85%, about 85%-90%, about 90%-95%, or any other reduction in
osteoclast activity or CTX serum levels in a range bounded by, or
between, any of these values.
[0164] In some embodiments, zoledronic acid in a disodium salt or
an acid form and other bisphosphonates including salt or acid form
may be used to treat Paget's disease of Bone or treat pain
associated with Paget's disease of bone and/or lower serum alkaline
phosphatase (ALP) levels. For example, the reduction of ALP levels
by at least about 20%, at least about 40%, at least about 50%, at
least about 60%, at least about 80%, about 50-60%, about 60-80%,
about 80-90%, about 90-95%, or any other reduction in ALP levels in
a range bounded by, or between, any of these values from baseline,
within 12 months, 18 months, or up to at least 5 years from the
time of the last oral administration of zoledronic acid or other
bisphosphonates. In some embodiments, when zoledronic acid in a
disodium salt or an acid form, or other bisphosphonate is
administered to treat the Paget's disease of bone or pain
associated with the Paget's disease of bone, the Paget's disease or
the pain associated with the Paget's disease has recurrence rate of
less than 20%, less than 10%, less than 5%, less than 1%, or does
not return within 12 months, 18 months, or 5 years, or more, from
the time of the last oral administration of zoledronic acid, or
other bisphosphonates.
[0165] In some embodiments, a dosage form comprising a disodium
salt of zoledronic acid is a solid.
[0166] In some embodiments, a dosage form comprising a disodium
salt of zoledronic acid is used to treat an inflammatory
condition.
[0167] In some embodiments, a dosage form comprising a disodium
salt of zoledronic acid is used to treat arthritis.
[0168] In some embodiments, a dosage form comprising a disodium
salt of zoledronic acid is used to treat complex regional pain
syndrome.
[0169] In some embodiments, zoledronic acid is in a form that has
an aqueous solubility, meaning the solubility in water, greater
than 1% (w/v), about 5% (w/v) to about 50% (w/v), about 5% (w/v) to
about 20% (w/v), about 10% (w/v) to about 15% (w/v), or about 12%
(w/v) to about 13% (w/v).
[0170] The disodium salt form of zoledronic acid can be more
compressible than the diacid form of zoledronic acid. This can make
it easier for a dosage form to have a desired hardness. It can also
make it easier to increase the drug load, so that a smaller tablet
can be given for a given dosage strength. In some embodiments, a
solid dosage form of zoledronic acid, such as the diacid form of
zoledronic acid or the disodium salt form of zoledronic acid, can
have a hardness of about 5 kPa to about 20 kPa or about 5 kPa to
about 14 kPa.
[0171] Zoledronic acid or another bisphosphonate may be combined
with a pharmaceutical carrier selected on the basis of the chosen
route of administration and standard pharmaceutical practice as
described, for example, in Remington's Pharmaceutical Sciences,
2005, the disclosure of which is hereby incorporated herein by
reference, in its entirety. The relative proportions of active
ingredient and carrier may be determined, for example, by the
solubility and chemical nature of the compounds, chosen route of
administration and standard pharmaceutical practice.
[0172] Zoledronic acid or another bisphosphonate may be
administered by any means that may result in the contact of the
active agent(s) with the desired site or site(s) of action in the
body of a patient. The compounds may be administered by any
conventional means available for use in conjunction with
pharmaceuticals, either as individual therapeutic agents or in a
combination of therapeutic agents. For example, they may be
administered as the sole active agents in a pharmaceutical
composition, or they can be used in combination with other
therapeutically active ingredients.
[0173] In some embodiments, an osteoclast inhibitor is
co-administered with a steroid. Suitable steroids include, for
example, hydrocortisone, hydrocortisone acetate, cortisone acetate,
tixocortol pivalate, prednisolone, methylprednisolone, prednisone,
triamcinolone acetonide, triamcinolone alcohol, mometasone,
amcinonide, budesonide, desonide, fluocinonide, fluocinolone
acetonide, halcinonide, betamethasone, betamethasone sodium
phosphate, dexamethasone, dexamethasone sodium phosphate,
fluocortolone, hydrocortisone-17-valerate, acleometasone
dipropionate, betamethasone valerate, betamethasone dippropionate,
prednicarbate, clobetasone-17-butyrate, clobetasol-17-propionate,
fluocortilone caproate, fluocortolone pivalate, and fluprednidene
acetate, hydrocortisone-17-butyrate, 17-aceponate, 17-buteprate,
and prednicarbate.
[0174] Any effective dose of steroid can be administered to a
person. In some embodiment, the dose of a steroid may be about
1-500 mg, 5-25 mg, about 1-3 mg, about 2-4 mg, about 3-5 mg, about
4-6 mg, about 5-7 mg, about 6-8 mg, about 7-9 mg, about 8-10 mg,
about 10-15 mg, about 10-20 mg, about 20-50 mg, about 50-100 mg,
about 100-200 mg, about 200-300 mg, about 300-400 mg, 400-500 mg
1-20 mg, about 10-30 mg, about 20-40 mg, about 30-50 mg, about
40-60 mg, about 50-70 mg, about 60-80 mg, about 70-90 mg, about
80-100 mg, about 90-110 mg, about 100-120 mg, about 110-130 mg,
about 120-140 mg, about 130-150 mg, about 140-160 mg, about 150-170
mg, about 160-180 mg, about 170-190 mg, about 180-200 mg, about
190-210 mg, about 200-220 mg, about 210-230 mg, about 220-240 mg,
about 230-250 mg, about 240-260 mg, about 250-270 mg, about 260-280
mg, about 270-290 mg, about 280-300 mg, about 290-310 mg, about
300-320 mg, about 310-330 mg, about 320-340 mg, about 330-350 mg,
about 340-360 mg, about 350-370 mg, about 360-380 mg, about 370-390
mg, about 380-300 mg, about 390-410 mg, about 400-420 mg, about
410-430 mg, about 420-440 mg, about 430-450 mg, about 440-460 mg,
about 450-470 mg, about 460-480 mg, about 470-490 mg, about 480-300
mg, about 490-510 mg of the steroid, or any amount in a range
bounded by any of these values.
[0175] The steroid can be given orally (for example, 7.5 mg of
prednisone), by a separate infusion (for example, 7.5 mg of methyl
prednisolone), mixed in with zoledronic acid in the same infusion,
or be administered intramuscularly, subcutaneously, by rectal
suppository, by inhalation, or injected directly into a joint.
[0176] Zoledronic acid or another bisphosphonate may be
administered to a human patient in a variety of forms adapted to
the chosen route of administration, e.g., orally, rectally, or
parenterally. Parenteral administration in this respect includes,
but is not limited to, administration by the following routes:
pulmonary, intrathecal, intravenous, intramuscular, subcutaneous,
intraocular, intrasynovial, transepithelial including transdermal,
sublingual and buccal; topically; nasal inhalation via
insufflation; and rectal systemic.
[0177] The effective amount of zoledronic acid or another
bisphosphonate will vary depending on various factors known to the
treating physicians, such as the severity of the condition to be
treated, route of administration, formulation and dosage forms,
physical characteristics of the bisphosphonate compound used, and
age, weight and response of the individual patients.
[0178] In some embodiments, the daily oral dose of pamidronate is
about 10 mg to about 1,000 mg, about 50 mg to about 500 mg, about
100 mg to about 500 mg, or about 150 mg to about 300 mg. In some
embodiments, the parenteral dose of pamidronate is about 5 mg to
about 500 mg, about 5 mg to about 200 mg, or about 10 mg to about
150 mg.
[0179] In some embodiments, the daily oral dose of neridronate is
about 10 mg to about 1,000 mg, about 50 mg to about 500 mg, about
100 mg to about 500 mg, or about 150 mg to about 300 mg. In some
embodiments, the parenteral dose of neridronate is about 5 mg to
about 500 mg, about 5 mg to about 200 mg, or about 10 mg to about
150 mg.
[0180] In some embodiments, the daily oral dose of alendronate is
about 0.5 mg to about 200 mg, about 1 mg to about 100 mg, about 5
mg to about 100 mg, or about 2 mg to about 50 mg. In some
embodiments, the parenteral dose of alendronate is about 1 mg to
about 100 mg, about 1 mg to about 40 mg, or about 2 mg to about 30
mg.
[0181] In some embodiments, the daily oral dose of olpadronate is
about 0.5 mg to about 200 mg, about 1 mg to about 100 mg, about 5
mg to about 100 mg, or about 2 mg to about 50 mg. In some
embodiments, the parenteral dose of olpadronate is about 1 mg to
about 100 mg, about 1 mg to about 40 mg, or about 2 mg to about 30
mg.
[0182] In some embodiments, the daily oral dose of ibandronate is
about 0.25 mg to about 100 mg, about 0.5 mg to about 50 mg, about
2.5 mg to about 50 mg, or about 1 mg to about 25 mg. In some
embodiments, the parenteral dose of ibandronate is about 0.5 mg to
about 50 mg, about 0.5 mg to about 20 mg, or about 1 mg to about 15
mg.
[0183] In some embodiments, the daily oral dose of risedronate is
about 0.25 mg to about 100 mg, about 0.5 mg to about 50 mg, about
2.5 mg to about 50 mg, or about 1 mg to about 25 mg. In some
embodiments, the parenteral dose of risedronate is about 0.25 mg to
about 25 mg, about 0.25 mg to about 10 mg, or about 0.5 mg to about
7.5 mg.
[0184] In some embodiments, the daily oral dose of zoledronate is
about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg, about
0.5 mg to about 10 mg, or about 0.2 mg to about 5 mg. In some
embodiments, the parenteral dose of zoledronate is about 0.25 mg to
about 25 mg, about 0.25 mg to about 10 mg, or about 0.5 mg to about
7.5 mg.
[0185] The dose of pamidronate, neridronate, olpadronate,
alendronate, ibandronate, risedronate, zoledronate or another
bisphosphonate compound may be administered in a single or divided
dose.
[0186] The amount of zoledronic acid or another bisphosphonate in a
therapeutic composition may vary. For example, some liquid
compositions may comprise about 0.0001% (w/v) to about 50% (w/v),
about 0.01% (w/v) to about 20% (w/v), about 0.01% to about 10%
(w/v), about 0.001% (w/v) to about 1% (w/v), about 0.1% (w/v) to
about 0.5% (w/v), about 1% (w/v) to about 3% (w/v), about 3% (w/v)
to about 5% (w/v), about 5% (w/v) to about 7% (w/v), about 7% (w/v)
to about 10% (w/v), about 10% (w/v) to about 15% (w/v), about 15%
(w/v) to about 20% (w/v), about 20% (w/v) to about 30% (w/v), about
30% (w/v) to about 40% (w/v), or about 40% (w/v) to about 50% (w/v)
of zoledronic acid.
[0187] Some solid compositions may comprise at least about 5%
(w/w), at least about 10% (w/w), at least about 20% (w/w), at least
about 50% (w/w), at least about 70% (w/w), at least about 80%,
about 10% (w/w) to about 30% (w/w), about 10% (w/w) to about 20%
(w/w), about 20% (w/w) to about 30% (w/w), about 30% (w/w) to about
50% (w/w), about 30% (w/w) to about 40% (w/w), about 40% (w/w) to
about 50% (w/w), about 50% (w/w) to about 80% (w/w), about 50%
(w/w) to about 60% (w/w), about 70% (w/w) to about 75% (w/w), about
70% (w/w) to about 80% (w/w), or about 80% (w/w) to about 90% (w/w)
of zoledronic acid.
[0188] Any suitable amount of an osteoclast inhibitor, including a
bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g.
zoledronic acid, minodronic acid, or ibandronic acid, may be used.
Some solid or liquid oral dosage forms, or units of oral dosage
forms (referred to collectively herein as "oral dosage form(s)")
may contain about 0.005 mg to about 20 mg, about 0.1 mg to about 10
mg, about 0.5 mg to about 10 mg, about 0.2 mg to about 5 mg, about
1 mg to about 500 mg, about 1 mg to about 50 mg, about 10 mg to
about 250 mg, about 100 mg to about 300 mg, about 20 mg to about
200 mg, about 20 mg to about 150 mg, about 30 mg to about 100 mg,
about 50 mg to about 200 mg, about 1 mg to about 1,000 mg, about 10
mg to about 50 mg, about 40 mg to about 60 mg, about 50 mg to about
60 mg, about 55 mg, about 10 mg to about 300 mg, about 10 mg to
about 150 mg, about 10 mg to about 100 mg, about 40 mg to about 150
mg, about 10 mg to about 600 mg, about 40 mg to about 600 mg, about
40 mg to about 2000 mg, about 40 mg to about 800 mg, about 25 mg to
about 800 mg, about 30 mg to about 800 mg, about 10 mg to about 500
mg, about 50 mg to about 150 mg, about 50 mg, about 100 mg, about
50 mg to about 500 mg, about 150 mg to about 200 mg, about 100 mg
to about 2000 mg, about 300 mg to about 1500 mg, about 200 mg to
about 1000 mg, about 100 mg to about 500 mg, about 160 mg, or about
150 mg of zoledronic acid in an acid form or in a salt form such as
disodium salt form, or any amount of osteoclast inhibitor in a
range bounded by, or between, any of these values. In some
embodiments, the oral osteoclast inhibitor is administered daily,
weekly, biweekly, monthly, every two or three months, once a year,
or twice a year.
[0189] Some oral dosage forms may contain about 0.005 mg to about
20 mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg,
about 0.2 mg to about 5 mg, about 1 mg to about 500 mg, about 1 mg
to about 50 mg, about 10 mg to about 250 mg, about 100 mg to about
300 mg, about 20 mg to about 200 mg, about 20 mg to about 150 mg,
about 30 mg to about 100 mg, about 1 mg to about 1,000 mg, about 10
mg to about 50 mg, about 40 mg to about 60 mg, about 50 mg to about
60 mg, about 55 mg, about 10 mg to about 300 mg, about 10 mg to
about 150 mg, about 10 mg to about 100 mg, about 40 mg to about 150
mg, about 10 mg to about 600 mg, about 40 mg to about 600 mg, about
40 mg to about 2000 mg, about 40 mg to about 800 mg, about 25 mg to
about 800 mg, about 30 mg to about 800 mg, about 10 mg to about 500
mg, about 50 mg to about 150 mg, about 50 mg, about 100 mg, about
50 mg to about 200 mg, about 50 mg to about 500 mg, about 150 mg to
about 200 mg, about 100 mg to about 2000 mg, about 300 mg to about
1500 mg, about 200 mg to about 1000 mg, about 100 mg to about 500
mg, about 160 mg, or about 150 mg of osteoclast inhibitor, or any
amount of osteoclast inhibitor in a range bounded by, or between,
any of these values. In some embodiments, the oral osteoclast
inhibitor is administered daily, weekly, bi-weekly, monthly, every
two or three months, once a year, or twice a year.
[0190] Any suitable amount of an osteoclast inhibitor, including a
bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g.
zoledronic acid, neridronate (neridronic acid), pamidronate,
olpadronate, alendronate, risedronate, minodronic acid, or
ibandronic acid, may be used. Some solid or liquid dosage forms, or
units of dosage forms may contain about 0.005 mg to about 20 mg,
about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about 0.2
mg to about 5 mg, about 1 mg to about 500 mg, about 1 mg to about
50 mg, about 10 mg to about 250 mg, about 100 mg to about 300 mg,
about 20 mg to about 200 mg, about 20 mg to about 150 mg, about 30
mg to about 100 mg, about 50 mg to about 200 mg, about 1 mg to
about 1,000 mg, about 10 mg to about 50 mg, about 40 mg to about 60
mg, about 50 mg to about 60 mg, about 55 mg, about 10 mg to about
300 mg, about 10 mg to about 150 mg, about 10 mg to about 100 mg,
about 40 mg to about 150 mg, about 10 mg to about 600 mg, about 40
mg to about 600 mg, about 40 mg to about 2000 mg, about 40 mg to
about 800 mg, about 25 mg to about 800 mg, about 30 mg to about 800
mg, about 10 mg to about 500 mg, about 50 mg to about 150 mg, about
50 mg, about 100 mg, about 50 mg to about 500 mg, about 150 mg to
about 200 mg, about 100 mg to about 2000 mg, about 300 mg to about
1500 mg, about 200 mg to about 1000 mg, about 100 mg to about 500
mg, about 160 mg, or about 150 mg of zoledronic acid in an acid
form or in a salt form such as disodium salt form, or any amount of
osteoclast inhibitor in a range bounded by, or between, any of
these values. In some embodiments, the oral or IV osteoclast
inhibitor is administered daily, every other day, every third day,
weekly, biweekly, monthly, every two or three months, every six
months, once a year, or twice a year from day 1.
[0191] Some dosage forms may contain about 0.005 mg to about 20 mg,
about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about 0.2
mg to about 5 mg, about 1 mg to about 500 mg, about 1 mg to about
50 mg, about 10 mg to about 250 mg, about 100 mg to about 300 mg,
about 20 mg to about 200 mg, about 20 mg to about 150 mg, about 30
mg to about 100 mg, about 1 mg to about 1,000 mg, about 10 mg to
about 50 mg, about 40 mg to about 60 mg, about 50 mg to about 65
mg, about 65 mg to about 70 mg, about 50 mg to about 60 mg, about
55 mg, about 10 mg to about 300 mg, about 10 mg to about 150 mg,
about 10 mg to about 100 mg, about 40 mg to about 150 mg, about 10
mg to about 600 mg, about 40 mg to about 600 mg, about 100 mg to
about 600 mg, about 40 mg to about 2000 mg, about 40 mg to about
800 mg, about 25 mg to about 800 mg, about 30 mg to about 800 mg,
about 10 mg to about 500 mg, about 50 mg to about 150 mg, about 50
mg, about 100 mg, about 50 mg to about 200 mg, about 50 mg to about
500 mg, about 150 mg to about 200 mg, about 100 mg to about 2000
mg, about 300 mg to about 1500 mg, about 200 mg to about 1000 mg,
about 100 mg to about 500 mg, about 160 mg, or about 150 mg of
osteoclast inhibitor, or any amount of osteoclast inhibitor in a
range bounded by, or between, any of these values. In some
embodiments, the oral or IV osteoclast inhibitor is administered
daily, every other day, every third day, weekly, bi-weekly,
monthly, every two or three months, every 6 months, once a year, or
twice a year from day 1.
[0192] In some embodiments, an oral dosage form may contain about
10 mg/m.sup.2 to about 20 mg/m.sup.2, about 15 mg/m.sup.2 to about
20 mg/m.sup.2, about 18 mg/m.sup.2, about 80 mg/m.sup.2 to about
150 mg/m.sup.2, about 90 mg/m.sup.2 to about 150 mg/m.sup.2, about
100 mg/m.sup.2 to about 150 mg/m.sup.2 of zoledronic acid, or any
amount of zoledronic in a range bounded by, or between, any of
these values. All dosage ranges or amounts expressed in mg/m.sup.2
are based upon the body surface area of the mammal.
[0193] In some embodiments, the daily oral dose of an osteoclast
inhibitor, including a bisphosphonate, such as a
nitrogen-containing bisphosphonate, e.g. zoledronic acid,
minodronic acid, or ibandronic acid, is about 0.005 mg to about 20
mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about
0.2 mg to about 5 mg, or any amount in a range bounded by, or
between, any of these values. In some embodiments, the daily oral
dose of osteoclast inhibitor is less than about 35 mg/m.sup.2, less
than about 30 mg/m.sup.2, less than about 25 mg/m.sup.2, about 1
mg/m.sup.2 to about 35 mg/m.sup.2, about 1 mg/m.sup.2 to about 30
mg/m.sup.2, about 1.5 mg/m.sup.2 to about 25 mg/m.sup.2, about 1.8
mg/m.sup.2 to about 20 mg/m.sup.2, about 10 mg/m.sup.2 to about 20
mg/m.sup.2, about 10 mg/m.sup.2 to about 30 mg/m.sup.2, about 15
mg/m.sup.2 to about 20 mg/m.sup.2, about 18 mg/m.sup.2, or any
amount of zoledronic acid in a range bounded by, or between, any of
these values.
[0194] In some embodiments, the daily oral dose of an osteoclast
inhibitor, including a bisphosphonate, such as a
nitrogen-containing bisphosphonate, e.g. zoledronic acid,
minodronic acid, or ibandronic acid, is about 0.005 mg to about 20
mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about
0.2 mg to about 5 mg, or any amount of osteoclast inhibitor in a
range bounded by, or between, any of these values. In some
embodiments, the daily oral dose of osteoclast inhibitor is less
than about 35 mg/m.sup.2, less than about 30 mg/m.sup.2, less than
about 25 mg/m.sup.2, about 1 mg/m.sup.2 to about 35 mg/m.sup.2,
about 1 mg/m.sup.2 to about 30 mg/m.sup.2, about 1.5 mg/m.sup.2 to
about 25 mg/m.sup.2, about 1.8 mg/m.sup.2 to about 20 mg/m.sup.2,
about 10 mg/m.sup.2 to about 20 mg/m.sup.2, about 10 mg/m.sup.2 to
about 30 mg/m.sup.2, about 15 mg/m.sup.2 to about 20 mg/m.sup.2,
about 18 mg/m.sup.2, or any amount of osteoclast inhibitor in a
range bounded by, or between, any of these values.
[0195] In some embodiments the daily oral dose of zoledronic acid
is about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg,
about 0.5 mg to about 10 mg, about 0.2 mg to about 5 mg, or any
amount of zoledronic acid in a range bounded by, or between, any of
these values. In some embodiments, the daily oral dose of
zoledronic acid is less than about 35 mg/m.sup.2, less than about
30 mg/m.sup.2, less than about 25 mg/m.sup.2, about 1 mg/m.sup.2 to
about 35 mg/m.sup.2, about 1 mg/m.sup.2 to about 30 mg/m.sup.2,
about 1.5 mg/m.sup.2 to about 25 mg/m.sup.2, about 1.8 mg/m.sup.2
to about 20 mg/m.sup.2, about 10 mg/m.sup.2 to about 20 mg/m.sup.2,
about 10 mg/m.sup.2 to about 30 mg/m.sup.2, about 15 mg/m.sup.2 to
about 20 mg/m.sup.2, about 18 mg/m.sup.2, or any amount of
zoledronic acid in a range bounded by, or between, any of these
values.
[0196] In some embodiments, the weekly oral dose of the osteoclast
inhibitor, including a bisphosphonate, such as a
nitrogen-containing bisphosphonate, e.g. zoledronic acid,
minodronic acid, ibandronic acid, is about 1 mg to about 1000 mg,
about 1 mg to about 500 mg, about 10 mg to about 250 mg, about 100
mg to about 300 mg, about 10 mg to about 100 mg, about 10 mg to
about 150 mg, about 10 mg to about 100 mg, about 10 mg to about 300
mg, about 20 mg to about 150 mg, about 20 mg to about 60 mg, about
30 mg to about 70 mg, about 40 mg to about 60 mg, about 50 mg to
about 70 mg, about 50 mg, about 55 mg, about 100 mg to about 150
mg, or about 30 mg to about 100 mg. In some embodiments, the weekly
oral dose of the osteoclast inhibitor is less than about 250
mg/m.sup.2, less than about 200 mg/m.sup.2, less than about 175
mg/m.sup.2, about 6 mg/m.sup.2 to about 250 mg/m.sup.2, about 10
mg/m.sup.2 to about 210 mg/m.sup.2, about 10 mg/m.sup.2 to about
170 mg/m.sup.2, about 4 mg/m.sup.2 to about 140 mg/m.sup.2, about
100 mg/m.sup.2 to about 140 mg/m.sup.2, about 126 mg/m.sup.2, or
any amount in a range bounded by, or between, any of these values.
The weekly oral dose may be given as a single dose, given once
during the week, or may be given in 2, 3, 4, 5, 6, or 7 individual
doses during the week.
[0197] In some embodiments the weekly oral dose of zoledronic acid
is about 1 mg to about 1000 mg, about 1 mg to about 500 mg, about
10 mg to about 250 mg, about 100 mg to about 300 mg, about 10 mg to
about 100 mg, about 10 mg to about 150 mg, about 10 mg to about 100
mg, about 10 mg to about 300 mg, about 20 mg to about 150 mg, about
20 mg to about 60 mg, about 30 mg to about 70 mg, about 40 mg to
about 60 mg, about 50 mg to about 70 mg, about 50 mg, about 55 mg,
about 100 mg to about 150 mg, or about 30 mg to about 100 mg. In
some embodiments, the weekly oral dose of zoledronic acid is less
than about 250 mg/m.sup.2, less than about 200 mg/m.sup.2, less
than about 175 mg/m.sup.2, about 6 mg/m.sup.2 to about 250
mg/m.sup.2, about 10 mg/m.sup.2 to about 210 mg/m.sup.2, about 10
mg/m.sup.2 to about 170 mg/m.sup.2, about 4 mg/m.sup.2 to about 140
mg/m.sup.2, about 100 mg/m.sup.2 to about 140 mg/m.sup.2, about 126
mg/m.sup.2, or any amount of zoledronic acid in a range bounded by,
or between, any of these values. The weekly oral dose may be given
as a single dose, given once during the week, or may be given in 2,
3, 4, 5, 6, or 7 individual doses during the week.
[0198] In some embodiments, the monthly dose of the osteoclast
inhibitor, including a bisphosphonate, such as a
nitrogen-containing bisphosphonate, e.g. zoledronic acid,
minodronic acid, or ibandronic acid, or the amount of the
osteoclast inhibitor that is administered over a period of a month,
is about 5000 mg or less, about 4000 mg or less, about 3000 mg or
less, about 2000 mg or less, about 1000 mg or less, about 700 mg or
less, about 600 mg or less, about 1 mg to about 4,000 mg, about 1
mg to about 1,000 mg, about 10 mg to about 1000 mg, about 50 mg to
about 1000 mg, about 10 mg to about 600 mg, about 40 mg to about
600 mg, about 50 mg to about 600 mg, about 40 mg to about 400 mg,
about 50 mg to about 200 mg, about 200 mg to about 300 mg, about
250 mg to about 350 mg, or about 100 mg to about 600 mg, about 40
mg to about 2000 mg, about 40 mg to about 800 mg, about 50 mg to
about 800 mg, or about 100 mg to about 800 mg, about 40 mg to about
1000 mg, about 50 mg to about 1000 mg, or about 100 mg to about
1000 mg, or any monthly dose in a range bounded by, or between, any
of these values. In some embodiments, the monthly oral dose of the
osteoclast inhibitor is less than about 1000 mg/m.sup.2, less than
about 800 mg/m.sup.2, less than about 600 mg/m.sup.2, about 10
mg/m.sup.2 to about 1000 mg/m.sup.2, about 50 mg/m.sup.2 to about
800 mg/m.sup.2, about 70 mg/m.sup.2 to about 700 mg/m.sup.2, about
100 mg/m.sup.2 to about 700 mg/m.sup.2, about 100 mg/m.sup.2 to
about 600 mg/m.sup.2, about 50 mg/m.sup.2 to about 200 mg/m.sup.2,
about 300 mg/m.sup.2 to about 600 mg/m.sup.2, about 450 mg/m.sup.2
to about 600 mg/m.sup.2, about 300 mg/m.sup.2 to about 1000
mg/m.sup.2, about 400 mg/m.sup.2 to about 1000 mg/m.sup.2, about
500 mg/m.sup.2 to about 1000 mg/m.sup.2, about 400 mg/m.sup.2 to
about 700 mg/m.sup.2, about 500 mg/m.sup.2 to about 600 mg/m.sup.2,
about 540 mg/m.sup.2, or any amount in a range bounded by, or
between, any of these values. A monthly dose may be given as a
single dose, or as two or more individual doses administered during
the month. In some embodiments, the monthly dose is administered in
2 or 3 weekly doses. In some embodiments, the monthly dose is
administered in 4 or 5 weekly doses. In some embodiments, the
monthly dose is administered in 28 to 31 daily doses. In some
embodiments, the monthly dose is administered in 5 to 10 individual
doses during the month. The monthly dose may be administered for
only 1 month, or may be repeatedly administered for 2 or more
months.
[0199] In some embodiments, the monthly dose of zoledronic acid, or
the amount of zoledronic acid that is administered over a period of
a month, is about 5000 mg or less, about 4000 mg or less, about
3000 mg or less, about 2000 mg or less, about 1000 mg or less,
about 700 mg or less, about 600 mg or less, about 1 mg to about
4,000 mg, about 1 mg to about 1,000 mg, about 10 mg to about 1000
mg, about 50 mg to about 1000 mg, about 10 mg to about 600 mg,
about 40 mg to about 600 mg, about 50 mg to about 600 mg, about 40
mg to about 400 mg, about 50 mg to about 200 mg, about 200 mg to
about 300 mg, about 250 mg to about 350 mg, or about 100 mg to
about 600 mg, about 40 mg to about 2000 mg, about 40 mg to about
800 mg, about 50 mg to about 800 mg, or about 100 mg to about 800
mg, about 40 mg to about 1000 mg, about 50 mg to about 1000 mg, or
about 100 mg to about 1000 mg, or any monthly dose in a range
bounded by, or between, any of these values. In some embodiments,
the monthly oral dose of zoledronic acid is less than about 1000
mg/m.sup.2, less than about 800 mg/m.sup.2, less than about 600
mg/m.sup.2, about 10 mg/m.sup.2 to about 1000 mg/m.sup.2, about 50
mg/m.sup.2 to about 800 mg/m.sup.2, about 70 mg/m.sup.2 to about
700 mg/m.sup.2, about 100 mg/m.sup.2 to about 700 mg/m.sup.2, about
100 mg/m.sup.2 to about 600 mg/m.sup.2, about 50 mg/m.sup.2 to
about 200 mg/m.sup.2, about 300 mg/m.sup.2 to about 600 mg/m.sup.2,
about 450 mg/m.sup.2 to about 600 mg/m.sup.2, about 300 mg/m.sup.2
to about 1000 mg/m.sup.2, about 400 mg/m.sup.2 to about 1000
mg/m.sup.2, about 500 mg/m.sup.2 to about 1000 mg/m.sup.2, about
400 mg/m.sup.2 to about 700 mg/m.sup.2, about 500 mg/m.sup.2 to
about 600 mg/m.sup.2, about 540 mg/m.sup.2, or any amount of
zoledronic acid in a range bounded by, or between, any of these
values. A monthly dose may be given as a single dose, or as two or
more individual doses administered during the month. In some
embodiments, the monthly dose is administered in 2 or 3 weekly
doses. In some embodiments, the monthly dose is administered in 4
or 5 weekly doses. In some embodiments, the monthly dose is
administered in 28 to 31 daily doses. In some embodiments, the
monthly dose is administered in 5 to 10 individual doses during the
month. The monthly dose may be administered for only 1 month, or
may be repeatedly administered for 2 or more months.
[0200] With respect to orally administering zoledronic acid to a
mammal, such as a dog, a rat, a rabbit, a monkey, an ape, or a
human being, doses of about 0.03 mg/kg to about 10 mg/kg, or any
smaller range within this range, such as about 0.4 mg/kg to about 3
mg/kg, about 0.4 mg/kg to about 1.5 mg/kg, mg/kg, about 0.4 mg/kg
to about 0.5 mg/kg, about 0.5 mg/kg to about 0.6 mg/kg, about 0.6
mg/kg to about 0.7 mg/kg, about 0.7 mg/kg to about 0.8 mg/kg, about
0.8 mg/kg to about 0.9 mg/kg, about 0.9 mg/kg to about 1 mg/kg,
about 1 mg/kg to about 1.1 mg/kg, about 1.1 mg/kg to about 1.2
mg/kg, about 1.2 mg/kg to about 1.3 mg/kg, about 1.3 mg/kg to about
1.4 mg/kg, about 1.4 mg/kg to about 1.5 mg/kg, about 1.5 mg/kg to
about 1.6 mg/kg, about 1.6 mg/kg to about 1.7 mg/kg, about 1.7
mg/kg to about 1.8 mg/kg, about 1.8 mg/kg to about 1.9 mg/kg, about
1.9 mg/kg to about 2 mg/kg, about 2 mg/kg to about 2.1 mg/kg, about
2.1 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about 2.3 mg/kg,
about 2.3 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.5
mg/kg, about 2.5 mg/kg to about 2.6 mg/kg, about 2.6 mg/kg to about
2.7 mg/kg, about 2.7 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to
about 2.9 mg/kg, about 2.9 mg/kg to about 3 mg/kg, about 3 mg/kg to
about 3.1 mg/kg, about 3.1 mg/kg to about 3.2 mg/kg, about 3.2
mg/kg to about 3.3 mg/kg, about 3.3 mg/kg to about 3.4 mg/kg, about
3.4 mg/kg to about 3.5 mg/kg, about 3.5 mg/kg to about 3.6 mg/kg,
about 3.6 mg/kg to about 3.7 mg/kg, about 3.7 mg/kg to about 3.8
mg/kg, about 3.8 mg/kg to about 3.9 mg/kg, about 3.9 mg/kg to about
4 mg/kg, about 0.4 mg/kg to about 0.6 mg/kg, about 0.6 mg/kg to
about 0.8 mg/kg, about 0.8 mg/kg to about 1 mg/kg, about 1 mg/kg to
about 1.2 mg/kg, about 1.2 mg/kg to about 1.4 mg/kg, about 1.4
mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.8 mg/kg, about
1.8 mg/kg to about 2 mg/kg, about 2 mg/kg to about 2.2 mg/kg, about
2.2 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.6 mg/kg,
about 2.6 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about 3
mg/kg, about 3 mg/kg to about 3.2 mg/kg, about 3.2 mg/kg to about
3.4 mg/kg, about 3.4 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to
about 3.8 mg/kg, about 3.8 mg/kg to about 4 mg/kg, about 0.4 mg/kg
to about 0.7 mg/kg, about 0.7 mg/kg to about 1 mg/kg, about 1 mg/kg
to about 1.3 mg/kg, about 1.3 mg/kg to about 1.6 mg/kg, about 1.6
mg/kg to about 1.9 mg/kg, about 1.9 mg/kg to about 2.2 mg/kg, about
2.2 mg/kg to about 2.5 mg/kg, about 2.5 mg/kg to about 2.8 mg/kg,
about 2.8 mg/kg to about 3 mg/kg, about 3.3 mg/kg to about 3.6
mg/kg, about 3.6 mg/kg to about 4 mg/kg, about 0.4 mg/kg to about 1
mg/kg, or about 0.5 mg/kg to about 1 mg/kg, may be a safe dose for
repeated oral administration, such as once daily dosing to once
yearly dosing, once daily dosing to twice yearly dosing, once daily
dosing to thrice yearly dosing, once daily dosing to dosing every
three months, once daily dosing to dosing every two months, once
daily dosing to dosing every two months, once daily dosing to
dosing every month, once daily dosing to dosing every 2-4 weeks,
once daily dosing to once weekly dosing, etc.
[0201] The doses referred to in the paragraph above for
administration of zoledronic acid to a mammal may be safely
administered 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15
times, or about 3 to about 10 times, once a day, or less
frequently, such as once week, once every two weeks, once a month,
etc.
[0202] For once daily to once weekly oral administration of
zoledronic acid to a mammal such as a mouse, rat, dog, primate, or
a human being, in some embodiments, a safely repeated dose may be
about 0.03 mg/kg to about 4 mg/kg, or any smaller range within this
range, such as about 0.01 mg/kg to about 0.02 mg/kg, about 0.02
mg/kg to about 0.03 mg/kg, about 0.03 mg/kg to about 0.04 mg/kg,
about 0.04 mg/kg to about 0.05 mg/kg, about 0.05 mg/kg to about
0.06 mg/kg, about 0.06 mg/kg to about 0.07 mg/kg, about 0.07 mg/kg
to about 0.08 mg/kg, about 0.08 mg/kg to about 0.09 mg/kg, about
0.09 mg/kg to about 0.1 mg/kg, about 0.1 mg/kg to about 0.11 mg/kg,
about 0.11 mg/kg to about 0.12 mg/kg, about 0.12 mg/kg to about
0.13 mg/kg, about 0.13 mg/kg to about 0.14 mg/kg, about 0.14 mg/kg
to about 0.15 mg/kg, about 0.15 mg/kg to about 0.16 mg/kg, about
0.16 mg/kg to about 0.17 mg/kg, about 0.17 mg/kg to about 0.18
mg/kg, about 0.18 mg/kg to about 0.19 mg/kg, about 0.19 mg/kg to
about 0.2 mg/kg, about 0.2 mg/kg to about 0.21 mg/kg, about 0.21
mg/kg to about 0.22 mg/kg, about 0.22 mg/kg to about 0.23 mg/kg,
about 0.23 mg/kg to about 0.24 mg/kg, about 0.24 mg/kg to about
0.25 mg/kg, about 0.25 mg/kg to about 0.26 mg/kg, about 0.26 mg/kg
to about 0.27 mg/kg, about 0.27 mg/kg to about 0.28 mg/kg, about
0.28 mg/kg to about 0.29 mg/kg, about 0.29 mg/kg to about 0.3
mg/kg, about 0.3 mg/kg to about 0.31 mg/kg, about 0.31 mg/kg to
about 0.32 mg/kg, about 0.32 mg/kg to about 0.33 mg/kg, about 0.33
mg/kg to about 0.34 mg/kg, about 0.34 mg/kg to about 0.35 mg/kg,
about 0.35 mg/kg to about 0.36 mg/kg, about 0.36 mg/kg to about
0.37 mg/kg, about 0.37 mg/kg to about 0.38 mg/kg, about 0.38 mg/kg
to about 0.39 mg/kg, about 0.39 mg/kg to about 0.4 mg/kg, about
0.05 mg/kg to about 0.2 mg/kg, about 0.05 mg/kg to about 0.15
mg/kg, about 0.06 mg/kg to about 0.15 mg/kg, about 0.07 mg/kg to
about 0.15 mg/kg, about 0.08 mg/kg to about 0.15 mg/kg, about 0.09
mg/kg to about 0.15 mg/kg, about 0.1 mg/kg to about 0.15 mg/kg,
about 0.03 mg/kg to about 0.5 mg/kg, about 0.06 mg/kg to about 0.2
mg/kg, about 0.07 mg/kg to about 0.2 mg/kg, about 0.08 mg/kg to
about 0.2 mg/kg, about 0.09 mg/kg to about 0.2 mg/kg, about 0.1
mg/kg to about 0.2 mg/kg, about 0.4 mg to about 4 mg, about 0.4
mg/kg to about 0.6 mg/kg, about 0.6 mg/kg to about 0.8 mg/kg, about
0.8 mg/kg to about 1 mg/kg, about 1 mg/kg to about 1.2 mg/kg, about
1.2 mg/kg to about 1.4 mg/kg, about 1.4 mg/kg to about 1.6 mg/kg,
about 1.6 mg/kg to about 1.8 mg/kg, about 1.8 mg/kg to about 2
mg/kg, about 2 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about
2.4 mg/kg, about 2.4 mg/kg to about 2.6 mg/kg, about 2.6 mg/kg to
about 2.8 mg/kg, about 2.8 mg/kg to about 3 mg/kg, about 3 mg/kg to
about 3.2 mg/kg, about 3.2 mg/kg to about 3.4 mg/kg, about 3.4
mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to about 3.8 mg/kg, about
3.8 mg/kg to about 4 mg/kg, about 0.5 mg/kg to about 2 mg/kg, about
0.6 mg/kg to about 2 mg/kg, about 0.7 mg/kg to about 2 mg/kg, about
0.8 mg/kg to about 2 mg/kg, about 0.5 mg/kg to about 1.5 mg/kg,
about 0.6 mg/kg to about 1.5 mg/kg, about 0.7 mg/kg to about 1.5
mg/kg, about 0.8 mg/kg to about 1.5 mg/kg, about 0.5 mg/kg to about
0.9 mg/kg, about 0.6 mg/kg to about 0.9 mg/kg, about 0.7 mg/kg to
about 0.9 mg/kg, about 0.5 mg/kg to about 1 mg/kg, about 0.6 mg/kg
to about 1 mg/kg, about 0.7 mg/kg to about 1 mg/kg, about 0.8 mg/kg
to about 1 mg/kg, or about 0.8 mg/kg to about 0.9 mg/kg.
[0203] For once weekly or less frequent oral administration of
zoledronic acid to a mammal such as a mouse, rat, dog, primate, or
a human being, in some embodiments, a safely repeated dose may be
about 0.4 mg to about 10 mg, or any smaller range within this
range, such as about 0.4 mg/kg to about 0.6 mg/kg, about 0.6 mg/kg
to about 0.8 mg/kg, about 0.8 mg/kg to about 1 mg/kg, about 1 mg/kg
to about 1.2 mg/kg, about 1.2 mg/kg to about 1.4 mg/kg, about 1.4
mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.8 mg/kg, about
1.8 mg/kg to about 2 mg/kg, about 2 mg/kg to about 2.2 mg/kg, about
2.2 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.6 mg/kg,
about 2.6 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about 3
mg/kg, about 3 mg/kg to about 3.2 mg/kg, about 3.2 mg/kg to about
3.4 mg/kg, about 3.4 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to
about 3.8 mg/kg, about 3.8 mg/kg to about 4 mg/kg, about 4 mg/kg to
about 4.2 mg/kg, about 4.2 mg/kg to about 4.4 mg/kg, about 4.4
mg/kg to about 4.6 mg/kg, about 4.6 mg/kg to about 4.8 mg/kg, about
4.8 mg/kg to about 5 mg/kg, about 5 mg/kg to about 5.2 mg/kg, about
5.2 mg/kg to about 5.4 mg/kg, about 5.4 mg/kg to about 5.6 mg/kg,
about 5.6 mg/kg to about 5.8 mg/kg, about 5.8 mg/kg to about 6
mg/kg, about 6 mg/kg to about 6.2 mg/kg, about 6.2 mg/kg to about
6.4 mg/kg, about 6.4 mg/kg to about 6.6 mg/kg, about 6.6 mg/kg to
about 6.8 mg/kg, about 6.8 mg/kg to about 7 mg/kg, about 7 mg/kg to
about 7.2 mg/kg, about 7.2 mg/kg to about 7.4 mg/kg, about 7.4
mg/kg to about 7.6 mg/kg, about 7.6 mg/kg to about 7.8 mg/kg, about
7.8 mg/kg to about 8 mg/kg, about 8 mg/kg to about 8.2 mg/kg, about
8.2 mg/kg to about 8.4 mg/kg, about 8.4 mg/kg to about 8.6 mg/kg,
about 8.6 mg/kg to about 8.8 mg/kg, about 8.8 mg/kg to about 9
mg/kg, about 9 mg/kg to about 9.2 mg/kg, about 9.2 mg/kg to about
9.4 mg/kg, about 9.4 mg/kg to about 9.6 mg/kg, about 9.6 mg/kg to
about 9.8 mg/kg, about 9.8 mg/kg to about 10 mg/kg, about 0.5 mg/kg
to about 2 mg/kg, about 0.6 mg/kg to about 2 mg/kg, about 0.7 mg/kg
to about 2 mg/kg, about 0.8 mg/kg to about 2 mg/kg, about 0.5 mg/kg
to about 1.5 mg/kg, about 0.6 mg/kg to about 1.5 mg/kg, about 0.7
mg/kg to about 1.5 mg/kg, about 0.8 mg/kg to about 1.5 mg/kg, about
0.5 mg/kg to about 1 mg/kg, about 0.6 mg/kg to about 1 mg/kg, about
0.7 mg/kg to about 1 mg/kg, about 0.8 mg/kg to about 1 mg/kg, or
about 0.8 mg/kg to about 0.9 mg/kg,
[0204] In some embodiments, the osteoclast inhibitor comprises
zoledronic acid, and the oral zoledronic acid, or disodium salt
thereof, may be administered in combination with about 0.1 mg to
about 10 mg of zoledronic acid, or a salt thereof, administered
parenterally, such as intravenously. In some embodiments, about 50
mg, about 100 mg, or about 150 mg of the disodium salt of
zoledronic acid is administered orally in combination with 1 mg
parenteral, such as intravenous, zoledronic acid. In some
embodiments the parenteral dose of zoledronic acid is about 0.25 mg
to about 25 mg, about 0.25 mg to about 10 mg, or about 0.5 mg to
about 7.5 mg.
[0205] With respect to oral administration of an osteoclast
inhibitor, such as zoledronic acid, minodronic acid, ibandronic
acid, or another bisphosphonate, for the treatment of pain
associated with inflammation, arthritis, CRPS, or any other
condition recited herein, it may helpful if the mammal or human
being to which the osteoclast inhibitor is administered does not
eat food or drink beverage, (other than any water required to
swallow the oral dosage form) for at least about 1 hour, at least
about 2 hours, at least about 4 hours, at least about 6 hours, at
least about 8 hours, at least about 10 hours, or at least about 12
hours before the osteoclast inhibitor is administered. It may also
be helpful if the mammal or human being to which the osteoclast
inhibitor is administered does not eat food or drink beverage for
at least about 30 minutes, at least about 1 hour, at least about 2
hours, at least about 3 hours, or at least about 4 hours after the
osteoclast inhibitor is administered. In some embodiments, a human
being to which the zoledronic acid is administered avoids lying
down, or remains upright or sits upright, for at least about 30
minutes or about 1 hour after receiving a dosage form containing
the osteoclast inhibitor. Avoiding food or beverage before or after
oral administration of the osteoclast inhibitor can improve the
bioavailability of the osteoclast inhibitor.
[0206] The oral bioavailability of osteoclast inhibitor in a dosage
form can vary. Some dosage forms may have ingredients added to
enhance the bioavailability. However, bioavailability enhancement
is not necessary for an oral dosage form to be effective. In some
embodiments, the dosage form is substantially free of
bioavailability-enhancing agents, such as amino acids or large
quantities (e.g. at least about 5%, 10%, 20%, 50%, 70%, or more) of
carboxylic acid salts. In some embodiments, an oral dosage form may
have an oral bioavailability of the osteoclast inhibitor--such as
zoledronic acid, minodronic acid, ibandronic acid--of about 0.01%
to about 10%, about 0.1% to about 7%, about 0.1% to about 5%, etc.
Without ingredients or other methods to enhance bioavailability,
bisphosphonates such as zoledronic acid typically have a low
bioavailability in an oral dosage form. In some embodiments, the
oral bioavailability of zoledronic acid is unenhanced or
substantially unenhanced. For example, the oral bioavailability of
zoledronic acid can be about 0.01% to about 5%, about 0.01% to
about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about
0.2% to about 2%, about 0.2% to about 1.5%, about 0.3% to about
1.5%, about 0.3% to about 1%, about 1% to about 3%, about 1.2% to
about 3.5%, about 1.2% to about 3%, about 1% to about 4%, about
1.5% to about 4.5%, about 0.1% to about 0.5%, about 0.3% to about
0.5%, about 0.5% to about 1%, about 0.6% to about 0.7%, about 0.7%
to about 0.8%, about 0.8% to about 0.9%, about 0.9%, about 1% to
about 1.1%, about 1.1% to about 1.2%, about 1.2% to about 1.3%,
about 1.3% to about 1.4%, about 1.4% to about 1.5%, about 1.5% to
about 1.6%, about 1.6% to about 1.8%, about 1.8% to about 2%, about
2% to about 2.2%, about 2.2% to about 2.4%, about 2.4% to about
2.6%, about 2.6% to about 2.8%, about 2.8% to about 3.0%, about 3%
to about 3.2%, about 3.2% to about 3.4%, about 3.4% to about 3.6%,
about 3.6% to about 3.8%, about 3.8% to about 4%, about 2% to about
2.5%, or any bioavailability of zoledronic acid in a range bounded
by, or between, any of these values.
[0207] One embodiment is a pharmaceutical composition comprising an
osteoclast inhibitor such as zoledronic acid, minodronic acid, or
ibandronic acid wherein the oral bioavailability of zoledronic acid
in the dosage form is from about 0.01% to about 10%.
[0208] In some embodiments, the oral bioavailability of the
osteoclast inhibitor in the dosage form is about 0.01% to about 5%,
about 0.1% to about 7%, about 0.1% to about 5%, about 0.1% to about
3%, about 0.1% to about 2%, about 0.2% to about 2%, about 0.2% to
about 1.5%, about 0.3% to about 1.5%, or about 0.3% to about
1.0%.
[0209] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.01% to about 5%.
[0210] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.1% to about 7%.
[0211] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.1% to about 5%.
[0212] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.1% to about 3%.
[0213] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.1% to about 2%.
[0214] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.2% to about 2%.
[0215] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.2% to about 1.5%.
[0216] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.3% to about 1.5%.
[0217] In some embodiments, the oral bioavailability of zoledronic
acid in the dosage form is about 0.3% to about 1.0%.
[0218] In some embodiments, an oral dosage form comprises about 10
mg to about 300 mg of zoledronic acid, minodronic acid, or
ibandronic acid and is administered daily for about 2 to about 15
consecutive days. This regimen may be repeated once monthly, once
every two months, once every three months, once every four months,
once every five months, once every six months, once yearly, or once
every two years.
[0219] In some embodiments, an oral dosage form comprises about 10
mg to about 150 mg or about 10 mg to about 100 mg of zoledronic
acid, minodronic acid, or ibandronic acid and is administered daily
for about 2 to about 15 consecutive days. This regimen may be
repeated once monthly, once every two months, once every three
months, once every four months, once every five months, once every
six months, once yearly, or once every two years.
[0220] In some embodiments, an oral dosage form comprises about 10
mg to about 150 mg or about 10 mg to about 100 mg of zoledronic
acid, minodronic acid, or ibandronic acid and is administered daily
for about 5 to about 10 consecutive days. This regimen may be
repeated once monthly, once every two months, once every three
months, once every four months, once every five months, once every
six months, once yearly, or once every two years.
[0221] In some embodiments, an oral dosage form comprises about 40
mg to about 150 mg of zoledronic acid, minodronic acid, or
ibandronic acid and is administered daily for about 5 to about 10
consecutive days. This regimen may be repeated once monthly, once
every two months, once every three months, once every four months,
once every five months, once every six months, once yearly, or once
every two years.
[0222] In some embodiments, the oral zoledronic acid, minodronic
acid, or ibandronic acid may be administered as one dose of about
100 mg to about 2000 mg. In some embodiments, the oral zoledronic
acid, minodronic acid, or ibandronic acid may be administered as
one dose of about 300 mg to about 1500 mg. In some embodiments, the
oral zoledronic acid, minodronic acid, or ibandronic acid may be
administered as one dose of about 200 mg to about 1000 mg. The dose
of zoledronic acid, minodronic acid, or ibandronic acid may be
administered in a single or divided dose.
[0223] An osteoclast inhibitor, such as zoledronic acid, minodronic
acid, or ibandronic acid, may be formulated for oral
administration, for example, with an inert diluent or with an
edible carrier, or it may be enclosed in hard or soft shell gelatin
capsules, compressed into tablets, or incorporated directly with
the food of the diet. For oral therapeutic administration, the
active compound may be incorporated with an excipient and used in
the form of ingestible tablets, buccal tablets, coated tablets,
troches, capsules, elixirs, dispersions, suspensions, solutions,
syrups, wafers, patches, and the like.
[0224] Tablets, troches, pills, capsules and the like may also
contain one or more of the following: a binder such as gum
tragacanth, acacia, corn starch or gelatin; an excipient, such as
dicalcium phosphate; a disintegrating agent such as corn starch,
potato starch, alginic acid and the like; a lubricant such as
magnesium stearate; a sweetening agent such as sucrose, lactose or
saccharin; or a flavoring agent such as peppermint, oil of
wintergreen or cherry flavoring. When the unit dosage form is a
capsule, it may contain, in addition to materials of the above
type, a liquid carrier. Various other materials may be present as
coating, for instance, tablets, pills, or capsules may be coated
with shellac, sugar or both. A syrup or elixir may contain the
active compound, sucrose as a sweetening agent, methyl and
propylparabens as preservatives, a dye and flavoring, such as
cherry or orange flavor. It may be desirable for material in a
dosage form or pharmaceutical composition to be pharmaceutically
pure and substantially non toxic in the amounts employed.
[0225] In some embodiments, an osteoclast inhibitor, including a
bisphosphonate, such as zoledronic acid, neridronic acid, etc., is
in a dosage form containing one of, or a combination of, the
ingredients in the Table E below.
TABLE-US-00002 TABLE E Iron Methyl Paraben Propyl Paraben Sorbitol
Carob Bean Gum Mannitol Gum Tragacanth Guar Gum Benzoic Acid Sodium
Benzoate Garlic and Oil of Garlic Oil of Rue Propyl Gallate Gum
Ghatti Gum Arabic Sterculia Gum (karaya gum) Indian Dill Seed Pulps
Clove Bud Extract Clove Bud Oil Clove Bud Oleoresin Clove Leaf Oil
Clove Stem Oil Cholic acid Desoxycholic acid Glycocholic acid Ox
bile extract Taurocholic acid Sorbose Sodium thiosulfate Gelatin
Mustard or Oil of Mustard (Brown and Yellow) Glycyrrhiza Ammoniated
Glycyrrhizin Licorice Caprylic Acid Stannous Chloride Ammonium
bicarbonate Ammonium carbonate Ammonium chloride Ammonium hydroxide
Ammonium phosphate, such as ammonium phosphate dibasic or Ammonium
phosphate monobasic Ammonium sulfate Calcium iodate Potassium
iodate Potassium iodide Aconitic Acid Calcium carbonate Potassium
bicarbonate Sodium bicarbonate Sodium carbonate Sodium
sesquicarbonate Glycerin and Glycerides Dextran Dextrins Corn
dextrins Calcium acetate Calcium chloride Calcium gluconate Calcium
phytate Calcium hydroxide Calcium oxide Succinic acid Butylated
Hydroxytoluene (BHT) Calcium hexametaphosphate Calcium phosphate
dibasic Calcium phosphate monobasic Calcium phosphate tribasic
Calcium pyrophosphate Phosphoric acid Potassium phosphate dibasic
Potassium phosphate monobasic Potassium phosphate tribasic
Potassium polymetaphosphate Potassium pyrophosphate Potassium
tripolyphosphate Sodium acid pyrophosphate Sodium hexametaphosphate
Sodium metaphosphate Sodium phosphate dibasic Sodium phosphate
monobasic Sodium phosphate tribasic Sodium pyrophosphate,
tetrabasic Sodium tetrametaphosphate Sodium tetraphosphate Sodium
trimetaphosphate Sodium tripolyphosphate Sulfuric Acid
alpha-Tocopherol acetate Tocopherols Choline Bitartrate Choline
Chloride Aluminum ammonium sulfate Aluminum hydroxide Aluminum
oleate Aluminum palmitate Aluminum potassium sulfate Aluminum
sodium sulfate Aluminum sulfate Sodium aluminate Sodium aluminum
phosphate, acidic Sodium aluminum phosphate, basic Sodium
phosphoaluminate Beeswax (yellow or white) Japan wax Carnauba wax
Corn Sugar (Dextrose) Corn Syrup Invert Sugar Inositol Calcium
stearate Hydrogenated tallow Stearic acid Tallow Malic acid L-Malic
acid Calcium sorbate Potassium sorbate Sodium sorbate Sorbic acid
Sulfamic acid Sodium hydrosulfite Zinc hydrosulfite Tall oil Fish
oil, hydrogenated Sucrose Agar-agar Ammonium alginate Calcium
alginate Potassium alginate Propylene glycol alginate Sodium
alginate Propylene Glycol Propylene glycol monostearate Brown algae
Red algae Calcium glycerophosphate Manganese glycerophosphate
Magnesium glycerophosphate Potassium glycerophosphate Potassium
hydroxide Sodium hydroxide Potassium metabisulfite Sodium bisulfite
Sodium metabisulfite Sodium sulfite Sulfur dioxide Magnesium
phosphate, dibasic Magnesium carbonate Magnesium chloride Magnesium
hydroxide Magnesium oxide Magnesium stearate Magnesium sulfate
Magnesium phosphate, tribasic Adipic acid Hydrogenated soybean oil
Ethyl formate Formic acid Sodium formate Carrageenan Nutmeg and
Mace Zinc acetate Zinc carbonate Zinc chloride Zinc oxide Zinc
sulfate Caramel Lard Lard oil Papain Gum guaiac Coconut oil
Linoleic acid Oleic acid Peanut oil Calcium hypophosphite Manganous
hypophosphite Potassium hypophosphite Sodium hypophosphite Pectin,
amidated Pectin, high ester Pectin, low acid Pectinates Pectinic
acid Carboxymethyl cellulose Cellulose acetate Ethyl cellulose
Hydroxypropylmethyl cellulose Methylcellulose Sodium Carboxymethyl
cellulose Rennet Tannic acid (hydrolyzable gallotannins) Acetic
acid Sodium acetate Sodium diacetate Pyridoxine Pyridoxine
hydrochloride Sodium oleate Sodium palmitate Ethyl acrylate,
monomeric Methyl acrylate, monomeric Ethyl acrylate, polymeric
Methyl acrylate, polymeric Bentonite Clay (kaolin) Corn silk
Ammonium citrate Calcium citrate Citric acid Isopropyl citrate
Potassium citrate Sodium citrate Stearyl citrate Triethyl citrate
Biotin Enzymatically hydrolyzed casein Acid hydrolyzed proteins
Enzymatically hydrolyzed protein Soy sauces Yeast autolyzates
Caffeine L-Glutamic acid L-Glutamic acid hydrochloride Monoammonium
L-glutamate Monopotassium L-glutamate Monosodium L-glutamate
Calcium Lactate L(+)-calcium lactate D(-)-Lactic acid Lactic acid
L(+)-lactic acid Butylated Hydroxyanisole (BHA) D- or DL-Calcium
pantothenate D-Pantothenyl alcohol D- or DL-Sodium pantothenate
Urea Thiamine hydrochloride Thiamine mononitrate Magnesium
gluconate Potassium gluconate Sodium gluconate Zinc gluconate
Vitamin B12 (cyanocobalamin) Vitamin D2 (ergocalciferol) Vitamin D3
(cholecalciferol) Potassium chloride
Sodium chloride Soy protein isolate Hydrochloric acid Copper
(cupric) gluconate Copper (cupric) sulfate Cuprous iodide Calcium
caseinate Casein Sodium caseinate Aluminum calcium silicate Calcium
silicate Diatomaceous earth (filter aid) Magnesium silicate Perlite
(filter aid) Potassium silicate Silica aerogel Silicon dioxides
Sodium aluminosilicate Sodium calcium aluminosilicate Sodium
silicate Talc (basic magnesium silicate) Tricalcium silicate
L(+)-potassium acid tartrate L(+)-sodium tartrate L(+)-tartaric
acid Manganous chloride Manganous citrate Manganous gluconate
Manganous oxide Manganous sulfate Lecithin Lecithin, hydrogen
peroxide bleached Riboflavin Riboflavin-5'-phosphate Calcium
propionate Dilauryl thiodipropionate Propionic acid Sodium
propionate Thiodipropionic acid Hydrogen peroxide Carbon dioxide
Nickel (elemental) Niacin (nicotinic acid) Niacinamide
(nicotinamide) Carotene (beta-carotene) L-Ascorbic acid Ascorbyl
palmitate (palmitoyl L-ascorbic) Calcium L-ascorbate Erythorbic
acid (D-isoascorbic acid) Sodium erythorbate (sodium
D-isoascorbate) Sodium L-ascorbate Acetylated Distarch Adipate
Acetylated Distarch Glycerol Acetylated Distarch Phosphate
Acetylated Distarch Oxypropanol Acid Modified Starch Arrowroot
Starch Bleached Starch Cornstarch Distarch Glycerol Distarch
Oxypropanol Distarch Phosphate High Amylose Cornstarch
Hydroxypropyl Distarch Glycerol Hydroxypropyl Distarch Phosphate
Hydroxypropyl Starch Hydroxypropyl Starch, oxidized Milo Starch
Monostarch Phosphate Potato starch Pregelatinized starch Rice
Starch Sodium Hydroxide Gelatinized Starch Starch Acetate Starch
Aluminum Octenyl Succinate Starch Sodium Succinate Starch Sodium
Octenyl Succinate Succinyl Distarch Glycerol Tapioca Starch Waxy
Maize Starch Wheat Starch Phosphated Distarch Phosphate Starch,
Sodium Hypochlorite oxidized Vitamin A Vitamin A acetate Vitamin A
palmitate Diacetyl Starter distillate Carbonyl Iron Carbonyl Iron
Electrolytic Iron Electrolytic Iron Ferric ammonium citrate Ferric
chloride Ferric citrate Ferric oxide Ferric phosphate Ferric
pyrophosphate Ferric sodium pyrophosphate Ferric sulfate Ferrous
ascorbate Ferrous carbonate Ferrous citrate Ferrous fumarate
Ferrous gluconate Ferrous lactate Ferrous sulfate Ferrous sulfate
Iron caprylate Iron linoleate Iron naphthenate Iron oxides Iron
peptonate Iron polyvinylpyrrolidone Iron tallate Sodium ferric EDTA
Sodium ferricitropyrophosphate Dietary Iron Ferric oxide Potassium
carbonate Calcium glycerophosphate Cellulose, such as
microcrystalline cellulose Titanium dioxide
[0226] Some compositions or dosage forms may be a liquid, or may
comprise a solid phase dispersed in a liquid.
[0227] An osteoclast inhibitor, such as zoledronic acid, minodronic
acid, or ibandronic acid may be formulated for parental or
intraperitoneal administration. Solutions of the active compounds
as free acids or pharmacologically acceptable salts can be prepared
in water suitably mixed with a surfactant, such as
hydroxypropylcellulose. A dispersion can also have an oil dispersed
within, or dispersed in, glycerol, liquid polyethylene glycols, and
mixtures thereof. Under ordinary conditions of storage and use,
these preparations may contain a preservative to prevent the growth
of microorganisms.
[0228] In some embodiments, an oral dosage form may comprise a
silicified microcrystalline cellulose such as PROSLOV.RTM.. For
example, about 20% (wt/wt) to about 70% (wt/wt), about 10% (wt/wt)
to about 20% (wt/wt), about 20% (wt/wt) to about 40% (wt/wt), about
25% (wt/wt) to about 30% (wt/wt), about 40% (wt/wt) to about 50%
(wt/wt), or about 45% (wt/wt) to about 50% (wt/wt) silicified
microcrystalline cellulose may be present in an oral dosage form or
a unit of an oral dosage form.
[0229] In some embodiments, an oral dosage form may comprise a
crosslinked polyvinylpyrrolidone such as crospovidone. For example,
about 1% (wt/wt) to about 10% (wt/wt), about 1% (wt/wt) to about 5%
(wt/wt), or about 1% (wt/wt) to about 3% (wt/wt) crosslinked
polyvinylpyrrolidone may be present in an oral dosage form or a
unit of an oral dosage form.
[0230] In some embodiments, an oral dosage form may comprise a
fumed silica such as AEROSIL.RTM.. For example, about 0.1% (wt/wt)
to about 10% (wt/wt), about 0.1% (wt/wt) to about 1% (wt/wt), or
about 0.4% (wt/wt) to about 0.6% (wt/wt) fumed silica may be
present in an oral dosage form or a unit of an oral dosage
form.
[0231] In some embodiments, an oral dosage form may comprise
magnesium stearate. For example, about 0.1% (wt/wt) to about 10%
(wt/wt), about 0.1% (wt/wt) to about 1% (wt/wt), or about 0.4%
(wt/wt) to about 0.6% (wt/wt) magnesium stearate may be present in
an oral dosage form or a unit of an oral dosage form.
[0232] An oral dosage form comprising zoledronic acid or another
bisphosphonate or osteoclast inhibitor may be included in a
pharmaceutical product comprising more than one unit of the oral
dosage form.
[0233] A pharmaceutical product containing oral dosage forms for
daily use can contain 28, 29, 30, or 31 units of the oral dosage
form for a monthly supply. An approximately 6 week daily supply can
contain 40 to 45 units of the oral dosage form. An approximately 3
month daily supply can contain 85 to 95 units of the oral dosage
form. An approximately six month daily supply can contain 170 to
200 units of the oral dosage form. An approximately one year daily
supply can contain 350 to 380 units of the oral dosage form.
[0234] A pharmaceutical product containing oral dosage forms for
weekly use can contain 4 or 5 units of the oral dosage form for a
monthly supply. An approximately two month weekly supply can
contain 8 or 9 units of the oral dosage form. An approximately six
week weekly supply can contain about 6 units of the oral dosage
form. An approximately three month weekly supply can contain 12, 13
or 14 units of the oral dosage form. An approximately six month
weekly supply can contain 22 to 30 units of the oral dosage form.
An approximately one year weekly supply can contain 45 to 60 units
of the oral dosage form.
[0235] A pharmaceutical product may accommodate other dosing
regimes. For example, a pharmaceutical product may comprise 5 to 10
units of the oral dosage form, wherein each unit of the oral dosage
form contains about 40 mg to about 150 mg of zoledronic acid,
minodronic acid, or ibandronic acid. Some pharmaceutical products
may comprise 1 to 10 units of the oral dosage form, wherein the
product contains about 200 mg to about 2000 mg of zoledronic acid,
minodronic acid, or ibandronic acid. For such a product, each unit
of the oral dosage form may be taken daily for 1 to 10 days or 5 to
10 days during a month, such as at the beginning of a month.
[0236] Some oral dosage forms comprising an osteoclast
inhibitor-such as suitable bisphosphonates like zoledronic acid,
minodronic acid, or ibandronic acid or salts thereof--may have
enteric coatings or film coatings. In some embodiments, an oral
dosage form of an osteoclast inhibitor comprises a tablet having an
enteric coating. In some embodiments, an oral dosage form of an
osteoclast inhibitor comprises a capsule having an enteric coating.
In some embodiments, an oral dosage form of an osteoclast inhibitor
comprises a tablet having a film coating. In some embodiments, an
oral dosage form of an osteoclast inhibitor comprises a capsule
having a film coating.
[0237] Useful doses for an antibody against RANK or RANKL, such as
denosumab, may range from about 0.1 mg/kg to about 20 mg/kg, about
0.75 mg/kg to about 7.5 mg/kg, about 0.1 mg/kg to about 5 mg/kg,
about 1 mg/kg to about 2 mg/kg, about 10 mg/kg to about 20 mg/kg,
about 12 to about 17 mg/kg, about 15 mg/kg to about 20 mg/kg, about
1 mg/kg, about 1 mg/kg to about 10 mg/kg, or any value bounded by
or in between these ranges based on the body weight of the mammal.
The chosen dose may be administered repeatedly, particularly for
chronic conditions, or the amount per dose may be increased or
decreased as treatment progresses. The chosen dose may be
administered one or more times per week, monthly, every two months,
every three months, every six months, or every year.
[0238] In some embodiments, 60 mg of denosumab is administered
subcutaneously to patient in need of treatment. In some
embodiments, the administration is repeated every six months.
[0239] There are a number of ways that some part of Compound 1
and/or Compound 2 may be removed from a zoledronic acid product.
For example, HPLC, preparative TLC, crystallization, sublimation,
or zone purification may be employed. Solvents that may be useful
in HPLC, TLC, or crystallization, may include, but are not limited
to, water or organic solvents, such as hexanes, diethyl ether,
ethyl acetate, methyl acetate, acetone, acetic acid, acetonitrile,
tetrahydrofuran, ethanol, methanol, isopropyl alcohol, chloroform,
diethyl ether, toluene, dimethylformamide, benzene, etc. Gradients,
or two solvent systems may be employed as well. For example, an
HPLC separation may begin by elution with water, after some time
eluting with water, an organic solvent, such as acetonitrile,
methanol, ethanol, ethyl acetate, acetone, acetic acid, methyl
acetate, or another solvent could gradually be added to the water,
or may replace the water entirely. Similarly, crystallization or
recrystallization may employ a single solvent, or a combination of
solvents. For example, zoledronic acid or a salt thereof, such as a
disodium salt, might be recrystallized from water, ethanol,
methanol, diethyl ether, methyl acetate, acetic acid, etc., or a
combination of these solvents or others. In some embodiments,
zoledronic acid or a salt thereof, such as a disodium salt, may be
dissolved in one solvent, such as water or acetic acid, and
crystallized by a second solvent or solvent system, such as hexane,
diethyl ether, chloroform, dichloromethane, ethyl acetate, methyl
acetate, acetic acid, ethanol, methanol, or a combination thereof.
In some embodiments, a disodium salt of zoledronic acid is
dissolved in water, and then crystallized by adding hexane. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding diethyl ether. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding chloroform. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding dichloromethane. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding ethyl acetate. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding methyl acetate. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding acetic acid. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding ethanol. In some
embodiments, a disodium salt of zoledronic acid is dissolved in
water, and then crystallized by adding methanol. For embodiments
employing water and a second solvent, the ratio of water to the
second solvent (water:second solvent) may be about 1:100 to about
100:1, about 1:10 to about 1:5, about 1:5 to about 1:4, about 1:4
to about 1:3, about 1:3 to about 1:2, about 1:2 to about 1:1, about
1:1 to about 2:1, about 2:1 to about 3:1, about 3:1 to about 4:1,
about 4:1 to about 5:1, or about 1:1 to about 10:1.
[0240] In some embodiments, a combination of two methods recited in
the paragraph above may be employed, such as HPLC or TLC and
crystallization. In some embodiments, a method may be repeated,
such as HPLC, preparative TLC, crystallization, sublimation, or
zone purification. In some embodiments, a purification method
recited in the paragraph above may be performed twice. In some
embodiments, a purification method recited in the paragraph above
may be performed three or four times.
[0241] Some oral dosage forms comprising zoledronic acid or a salt
thereof may have enteric coatings or film coatings.
[0242] In the examples below, zoledronic acid was administered in
the disodium salt form as disodium zoledronate tetrahydrate. No
bioavailability enhancing agents were used in the test
compositions.
Example 1
Effect of Orally Administered Zoledronic Acid in Rat Model of
Inflammatory Pain
Method:
[0243] The effect of orally administered zoledronic acid on
inflammatory pain was examined using the rat complete Freund's
adjuvant (CFA) model. Inflammatory pain was induced by injection of
100% CFA in a 75 .mu.L volume into the left hind paws of
Sprague-Dawley.RTM. rats on day 0, followed by assessments on days
1-3. Animals were orally administered vehicle (control), zoledronic
acid 18 mg/m.sup.2 (or 3 mg/kg), zoledronic acid 120 mg/m.sup.2 (or
20 mg/kg), or zoledronic acid 900 mg/m.sup.2 (or 150 mg/kg) daily
on days 1-3. Drug was dissolved in distilled water and prepared
fresh daily. Animals were fasted prior to dosing. Under current FDA
guidelines for extrapolating starting dosages from animals to
humans, dosages expressed in mg/m.sup.2 are considered equivalent
between mammalian species. Thus, for example, 18 mg/m.sup.2 in a
rat is considered equivalent to 18 mg/m.sup.2 in a human being,
while 3 mg/kg in a rat may not be equivalent to 3 mg/kg in a human
being.
[0244] Values for inflammatory pain (mechanical hyperalgesia) in
the vehicle and drug-treated animals were obtained on day 0 prior
to CFA injection, and at baseline and post-treatment on days 1-3.
Pain was assessed using a digital Randall-Selitto device (dRS; IITC
Life Sciences, Woodland Hills, Calif.). Animals were placed in a
restraint sling that suspended the animal, leaving the hind limbs
available for testing. Paw compression threshold was measured by
applying increasing pressure to the plantar surface of the hind paw
with a dome-shaped tip placed between the 3rd and 4th metatarsus.
Pressure was applied gradually over approximately 10 seconds.
Measurements were taken from the first observed nocifensive
behavior of vocalization, struggle or withdrawal. A cut-off value
of 300 g was used to prevent injury to the animal.
[0245] Reversal of inflammatory pain was calculated according to
the formula:
% reversal=(Post-treatment-Post-CFA baseline)/(Pre-CFA
baseline-Post-CFA baseline).times.100.
[0246] The experiment was carried out using 9-10 animals per
group.
Results:
[0247] Oral administration of zoledronic acid significantly
improved inflammatory pain thresholds compared to vehicle. Pain
threshold measurements taken at various times are shown in FIG. 1.
Paw compression thresholds in the 18 mg/m.sup.2 group were higher
than for vehicle during the entire measurement period after 30
minutes from the start of treatment. On day three, paw compression
thresholds for both the 18 mg/m.sup.2 and 900 mg/m.sup.2 groups
were greater than for vehicle. An improvement in pain threshold of
49% and 83% from baseline was observed for the 18 mg/m.sup.2 and
the 900 mg/m.sup.2 groups respectively.
[0248] Orally administered zoledronic acid produced a 29% reversal
of inflammatory pain at the 18 mg/m.sup.2, and a 48% reversal at
the 900 mg/m.sup.2 dose. This magnitude of effect is comparable to
that obtained with clinical doses of commercially available NSAIDs
when tested in a similar model of inflammatory pain. Under current
FDA guidelines, the reference body surface area of a human adult is
1.62 m.sup.2. Thus, a daily dose of 18 mg/m.sup.2 corresponds to a
monthly dose of about 500-560 mg/m.sup.2 or a human dose of about
800-900 mg.
[0249] Surprisingly, the two higher doses resulted in thresholds
that were lower than vehicle on the first two days of dosing. The
120 mg/m.sup.2 group was approximately equal or inferior to vehicle
at all time points during the assessment period. While the 900
mg/m.sup.2 group showed effectiveness on day 3, this result was
accompanied by significant toxicity necessitating euthanization of
all the animals in this group two days after cessation of
dosing.
Example 2
Effect of Orally Administered Zoledronic Acid in Rat Model of
Arthritis Pain
Method:
[0250] The effect of orally administered zoledronic acid on
arthritis pain was examined in the rat complete Freund's adjuvant
(CFA) model of arthritis pain. In this model, injection of 100%
complete Freund's adjuvant (CFA) in a 75 .mu.L volume into the left
hind paws is followed by a 10-14 day period to allow for the
development of arthritis pain. Animals were orally administered
vehicle (control), zoledronic acid 54 mg/m.sup.2 (or 9 mg/kg), or
zoledronic acid 360 mg/m.sup.2 (or 60 mg/kg), divided in three
equal daily doses on the first three days post CFA injection. Drug
was dissolved in distilled water and prepared fresh daily. Animals
were fasted prior to dosing.
[0251] Arthritis pain (mechanical hyperalgesia) in the vehicle and
drug-treated animals was evaluated on day 14 post CFA injection
using a digital Randall-Selitto device (dRS; IITC Life Sciences,
Woodland Hills, Calif.). Animals were placed in a restraint sling
that suspended the animal, leaving the hind limbs available for
testing. Paw compression threshold was measured by applying
increasing pressure to the plantar surface of the hind paw with a
dome-shaped tip placed between the 3rd and 4th metatarsus. Pressure
was applied gradually over approximately 10 seconds. Measurements
were taken from the first observed nocifensive behavior of
vocalization, struggle or withdrawal. A cut-off value of 300 g was
used to prevent injury to the animal.
[0252] Reversal of arthritis pain in the ipsilateral (CFA-injected)
paw was calculated according to the formula:
% reversal=(ipsilateral drug threshold-ipsilateral vehicle
threshold)/(contralateral vehicle threshold-ipsilateral vehicle
threshold).times.100.
[0253] The experiment was carried out using 7-10 animals per group.
Results:
[0254] Oral administration of zoledronic acid significantly
improved arthritis pain thresholds compared to vehicle. As shown in
FIGS. 2A and 2B, orally administered zoledronic acid produced a
dose-dependent reversal of arthritis pain. A reversal of 33% was
observed in the 54 mg/m.sup.2 group, and reversal of 54% was
observed in the 360 mg/m.sup.2 group. Under current FDA guidelines,
the reference body surface area of a human adult is 1.62 m.sup.2.
Thus, 54 mg/m.sup.2 in a rat is equivalent to an implied human dose
of about 87 mg, and 360 mg/m.sup.2 in a rat is equivalent to an
implied human dose of about 583 mg.
Example 3. Treatment of Complex Regional Pain Syndrome with Orally
Administered Zoledronic Acid
[0255] The effect of orally administered zoledronic acid was
examined in the rat tibia fracture model of complex regional pain
syndrome (CRPS). CRPS was induced in the rats by fracturing the
right distal tibias of the animals and casting the fractured
hindpaws for 4 weeks, as described in Guo T Z et al. (Pain. 2004;
108: 95-107). This animal model has been shown to replicate the
inciting trauma (such as a fracture, a surgery, a crushing injury,
a cutting injury, a scratch, or a puncture injury), natural
history, signs, symptoms, and pathologic changes observed in human
CRPS patients (Kingery W S et al., Pain. 2003; 104:75-84).
[0256] Animals were orally administered either vehicle (control) or
zoledronic acid, in a dosage of 18 mg/m.sup.2/day (3 mg/kg/day) for
28 days, starting on the day of fracture and casting. Drug was
dissolved in distilled water and administered by gavage. Animals
were fasted for 4 hours before and 2 hours after dosing. At the end
of the 28-day period, casts were removed, and on the following day,
the rats were tested for hindpaw pain, edema, and warmth.
Pain Assessments
[0257] Pain was assessed by measuring hyperalgesia, and weight
bearing.
[0258] To measure hyperalgesia, an up-down von Frey testing
paradigm was used. Rats were placed in a clear plastic cylinder (20
cm in diameter) with a wire mesh bottom and allowed to acclimate
for 15 minutes. The paw was tested with one of a series of eight
von Frey hairs ranging in stiffness from 0.41 g to 15.14 g. The von
Frey hair was applied against the hindpaw plantar skin at
approximately midsole, taking care to avoid the tori pads. The
fiber was pushed until it slightly bowed and then it was jiggled in
that position for 6 seconds. Stimuli were presented at an interval
of several seconds. Hindpaw withdrawal from the fiber was
considered a positive response. The initial fiber presentation was
2.1 g and the fibers were presented according to the up-down method
of Dixon to generate six responses in the immediate vicinity of the
50% threshold. Stimuli were presented at an interval of several
seconds.
[0259] An incapacitance device (IITC Inc. Life Science, Woodland,
Calif., USA) was used to measure hindpaw weight bearing, a postural
effect of pain. The rats were manually held in a vertical position
over the apparatus with the hindpaws resting on separate metal
scale plates and the entire weight of the rat was supported on the
hindpaws. The duration of each measurement was 6 seconds and 10
consecutive measurements were taken at 60-second intervals. Eight
readings (excluding the highest and lowest ones) were averaged to
calculate the bilateral hindpaw weight-bearing values. Weight
bearing data were analyzed as the ratio between right (fracture)
and left hindpaw weight bearing values ((2R/(R+L)).times.100%).
Edema Assessment
[0260] A laser sensor technique was used to determine the
dorsal-ventral thickness of the hindpaw. Before baseline testing
the bilateral hindpaws were tattooed with a 2 to 3 mm spot on the
dorsal skin over the midpoint of the third metatarsal. For laser
measurements each rat was briefly anesthetized with isoflurane and
then held vertically so the hindpaw rested on a table top below the
laser. The paw was gently held flat on the table with a small metal
rod applied to the top of the ankle joint. Using optical
triangulation, a laser with a distance measuring sensor was used to
determine the distance to the table top and to the top of the
hindpaw at the tattoo site and the difference was used to calculate
the dorsal-ventral paw thickness. The measurement sensor device
used in these experiments (4381 Precicura, Limab, Goteborg, Sweden)
has a measurement range of 200 mm with a 0.01 mm resolution.
Hindpaw Temperature Measurement
[0261] The temperature of the hindpaw was measured using a fine
wire thermocouple (Omega, Stanford, Conn., USA) applied to the paw
skin. Six sites were tested per hindpaw. The six measurements for
each hindpaw were averaged for the mean temperature.
Results
[0262] As illustrated in FIG. 3, treatment with orally administered
zoledronic acid reversed pain, restored weight bearing, and
prevented edema as compared to vehicle treated animals.
[0263] As illustrated in FIG. 4, von Frey pain thresholds for the
right (fracture) hindpaw were reduced by 72% versus the
contralateral (normal) hindpaw in vehicle treated animals.
Zoledronate treatment reversed fracture induced pain by 77% as
compared to vehicle treatment.
[0264] As illustrated in FIG. 5, reduction in weight bearing, a
postural effect of pain, was significantly higher in the vehicle
treated group as compared to the zoledronic acid treated group.
Weight bearing on the fracture hindlimb was reduced to 55% of
normal in the vehicle treated group. Zoledronate treatment
significantly restored hindlimb weight bearing as compared to
vehicle treatment (86% of normal).
[0265] As illustrated in FIG. 6, the expected increase in hindpaw
thickness was greater in the vehicle treated group as compared to
the zoledronic acid treated group, reflecting the development of
edema. Zoledronate treatment reduced hindpaw edema by 60% versus
vehicle treatment.
[0266] Zoledronic acid reduced hindpaw warmth by 5% versus vehicle
treatment.
[0267] The daily dose in the above experiment was 18
mg/m.sup.2/day. Under current FDA guidelines, the reference body
surface area of a human adult is 1.62 m.sup.2. Thus, a daily dose
of 18 mg/m.sup.2 corresponds to a monthly dose of about 500-560
mg/m.sup.2 or a human dose of about 800-900 mg.
Example 4. Solubility of Disodium Salt of Zoledronic Acid
[0268] The aqueous solubility of zoledronic acid and disodium
zoledronate tetrahydrate was determined. One gram of the test
compound was measured in to a beaker. Demineralized water (pH 5.5)
was then added in small increments to the test compound, and
sonication was applied to the mixture. The procedure was continued
until complete dissolution was achieved. Full dissolution was
determined to have been reached when a clear solution was present
with no visible material. The volume of water required to reach
full dissolution was used to calculate a solubility value expressed
in grams per 100 mL. The procedure was performed for each
compound.
Results
[0269] As shown in FIG. 7, the aqueous solubility of disodium
zoledronate tetrahydrate is approximately 50 times that of
zoledronic acid. Disodium zoledronate tetrahydrate has a solubility
of 12.5 g/100 mL compared to only 0.25 g/100 mL for zoledronic
acid.
Example 5. Bioavailability of Orally Administered Zoledronic Acid
and Disodium Zoledronate
[0270] Tablets were manufactured containing either pure zoledronic
acid or the disodium salt of zoledronic acid (disodium zoledronate
tetrahydrate). Both types of tablets contained 50 mg of zoledronic
acid equivalent per tablet. Identical excipients were used in both
types of tablets, with amounts adjusted to account for the
difference in molecular weights between the acid and the disodium
salt.
[0271] Beagle dogs were orally administered tablets containing 150
mg zoledronic acid equivalent either in the form of disodium
zoledronate (Group 1) or pure zoledronic acid (Group 2). Each
animal was given three 50 mg equivalent tablets (150 mg total),
which were administered together. The animal's oral cavity was
wetted with water before placing the tablets on the back of the
animal's tongue. Animals were fasted before and after dosing.
Animals were 6 to 9 months of age and weighed 6 to 10 kg on the day
of dosing. There were three dogs per group.
[0272] Serial blood samples were collected from each animal by
venipuncture of the jugular vein at various points after dosing for
measurement of plasma concentrations of zoledronic acid. Blood
samples were collected into chilled tubes containing K.sub.2EDTA as
the anticoagulant. Samples were then centrifuged at approximately
3000 rpm at +4.degree. C. for 10 minutes for plasma derivation.
Plasma concentrations of zoledronic acid were measured using an
LC/MS/MS method.
Results
[0273] The average plasma concentrations of zoledronic acid for
each group of dogs is summarized in Table 1 and illustrated in FIG.
8. Detectable plasma levels of zoledronic acid were observed for
the entire 48 hours that they were measured.
TABLE-US-00003 TABLE 1 Zoledronic Acid plasma concentrations in
beagle dogs Plasma Time concentration (hour) (ng/mL) Group 1 (N =
3) Disodium Zoledronate 0 0.00 Tablets 0.25 1193.97 (150 mg acid
equivalent) 0.5 1852.12 0.75 1776.51 1 1626.56 2 640.57 4 136.93 6
53.11 8 26.97 12 13.74 24 6.78 48 5.39 Group 2 (N = 3) Zoledronic
Acid Tablets 0 0.00 (150 mg acid equivalent) 0.25 390.92 0.5 846.19
0.75 819.15 1 831.77 2 477.76 4 90.11 6 28.22 8 15.10 12 6.13 24
3.18 48 1.84
[0274] Disodium zoledronate produced significantly higher plasma
levels of zoledronic acid than pure zoledronic acid, indicating
improved oral absorption with the salt form. Measured using peak
plasma concentrations (C.sub.max), the disodium salt resulted in a
119% actual and 74% weight-adjusted increase in bioavailability as
compared to pure zoledronic acid. Measured using area under the
plasma concentration curve (AUC.sub.0-.infin.), bioavailability was
84% and 46% greater with the disodium salt than with pure
zoledronic acid, on an actual and weight-adjusted basis
respectively. The average AUC.sub.0-.infin. for the disodium salt
was 4073 ngh/mL and the average AUC.sub.0-.infin. for the diacid
was 2217 ngh/mL. The AUC.sub.0-.infin. was found to be dose
proportional. Thus, for beagle dogs similar to those tested, about
3 mg to about 4 mg of the disodium salt would be expected to result
in an AUC.sub.0-.infin. of about 100 ngh/mL, and about 7 mg to
about 8 mg of the disodium salt would be expected to result in an
AUC.sub.0-.infin. of about 200 ngh/mL.
Example 6. Hardness of Tablets Comprising Zoledronic Acid in the
Free Acid and Disodium Salt Forms
[0275] Tablets were prepared by blending zoledronic acid, either in
the form of the free acid or the disodium salt, with identical
excipients. For dosage forms with a greater amount of active, the
amount of the excipients was reduced proportionally to keep the
weight of the tablet at about 100 mg. After blending, the
ingredients were compressed at varying pressures, followed by a
film coating. The resulting tablets were then tested for hardness
using a Dr. Schleuniger Pharmatron 8M Tablet Hardness Tester. The
results are shown in Table 2 and FIG. 9.
TABLE-US-00004 TABLE 2 Hardness (kPa) Compression Disodium Disodium
Force Diacid Salt Salt (psi) 50 mg 50 mg 71 mg 800 4.0 8.7 4.8 1100
6.1 11.2 6.8 1500 7.7 13.7 7.4 2000 8.7 16.3 10.7 2400 8.7 11.3
3000 11.4 14.1 4400 12.5 14.9 5500 12.8 18.2 6100 13.0
Example 7. Effects of Zoledronic Acid on Patients with
Osteoarthritis and BML
[0276] Some embodiments related to joint pain, bone marrow lesions,
and osteoarthritis were conceived as a result of analyzing data
from a clinical study. Some of the results of this study were
reported by Laslett et al. in Ann Rheum Dis 2012; 71:1322-1328.
Some of the description and data reported below was not published
prior to filing the present application. Fifty-two (52) patients
with clinical knee osteoarthritis and knee bone marrow lesions
(BML) were randomized to receive either intravenous zoledronic acid
(5 mg) or placebo in a double blind fashion. All patients had to
have at least one bone marrow lesion (BML) in the affected knee on
magnetic resonance imaging (MRI). All patients had x-ray of the
knee for determination of joint space narrowing (JSN), which was
graded according to the Osteoarthritis Research Society
International (OARSI) atlas. Patients had either no joint space
narrowing (OARSI Grade 0), or greater degrees of joint space
narrowing (OARSI Grade 1 and Grade 2). Twenty six patients were
treated with zoledronic acid (8, 6, and 12 with OARSI Grades 0, 1,
and 2, respectively). Twenty six patients received placebo (8, 8,
and 10 with OARSI Grades 0, 1 and 2, respectively).
[0277] Pain intensity was assessed, at baseline and at three
months, using a 100 mm visual analog scale (VAS), with zero
representing no pain and 100 representing extreme pain. The change
in pain intensity from baseline to 3 months was calculated.
[0278] With zoledronic acid treatment, pain was reduced
significantly as compared to placebo in patients with no joint
space narrowing (OARSI Grade 0), but not in patients with joint
space narrowing (OARSI Grades 1-2). As shown in Table 3 and FIG.
10, average VAS scores were reduced by 15 mm as compared to placebo
in the OARSI Grade 0 group, but only by 0.28 as compared to placebo
in patients with OARSI Grades 1-2.
[0279] In the zoledronic acid group, average VAS scores at 3 months
decreased from baseline by approximately 25 mm and 21 mm in
patients with OARSI Grades 0 and 1, respectively, but only by 9 mm
in the OARSI Grade 2 patients (FIG. 11).
TABLE-US-00005 TABLE 3 Change in VAS Pain Scores at Three Months by
OARSI Grade (mm) OARSI Grade 0 OARSI Grades 1-2 Zoledronic Acid
-24.6 -13.2 Placebo -9.6 -12.9 Difference from Placebo -15.0
-0.28
[0280] With zoledronic acid treatment, pain was reduced
significantly as compared to placebo in patients with baseline VAS
pain intensity scores of 50 mm or greater, but not in patients with
baseline VAS scores less than 50 mm. As shown in Table 4, average
VAS scores were reduced by 9 mm as compared to placebo in the
patients with baseline VAS.gtoreq.50 mm, but only by 0.6 as
compared to placebo in patients with baseline VAS<50 mm.
TABLE-US-00006 TABLE 4 Change in VAS Pain Scores at Three Months by
Baseline VAS (mm) Baseline VAS .gtoreq.50 mm Baseline VAS <50 mm
Zoledronic Acid -26.2 -7.3 Placebo -17.2 -6.7 Difference from -9.0
-0.6 Placebo
[0281] As summarized in Table 5 and illustrated in FIG. 12, pain
reduction was greater in patients with baseline VAS 3>50 mm,
greater still in patients with OARSI Grade 0 joint space narrowing,
and greatest in patients with both baseline VAS 3>50 mm and
OARSI Grade 0 joint space narrowing.
TABLE-US-00007 TABLE 5 Pain Reduction Compared to Placebo at Three
Months (mm) VAS Change All patients -4.8 Baseline VAS .gtoreq.50 mm
-9.0 OARSI Grade 0 -15.0 Baseline VAS .gtoreq.50 mm + OARSI Grade 0
-19.4
[0282] BMLs were evaluated using proton density-weighted fat
saturation MR images. BMLs were scored using Osiris software
(University of Geneva, Geneva, Switzerland). The maximum size was
measured in mm.sup.2 using software cursors applied to the greatest
area of each lesion. The lesion with the highest score was used if
more than one was present at the same site. Each patient was given
a BML score (mm.sup.2) at each of the four sites (medial tibial,
medial femoral, lateral tibial, and lateral femoral sites) and
these were summed to create a total BML score (mm.sup.2). The
change in the total area of BMLs from baseline to 6 months was
calculated.
[0283] The size of BMLs was reduced with zoledronic acid treatment.
As shown in FIG. 13 and Table 6, average BML area decreased by
approximately 190 mm.sup.2 as compared to placebo in the OARSI
Grade 0 group, but only by approximately 33 mm.sup.2 as compared to
placebo in patients with OARSI Grades 1-2.
TABLE-US-00008 TABLE 6 Change in BML Size (mm.sup.2) OARSI Grade 0
OARSI Grades 1-2 Zoledronic Acid -244 -117 Placebo -55 -84
Difference from Placebo -190 -33
Example 8
Methods
[0284] A study was performed to evaluate the efficacy of a single
intravenous infusion of 5 mg ZA in comparison with intravenous
placebo infusion among patients with chronic low back pain (LBP)
and Modic changes on MRI. This study was a double-blinded,
randomized, placebo-controlled clinical trial in patients with low
back pain (LBP). Patients were included in the study if they had
low back symptoms for at least three months, a LBP of at least six
(6) on a 10-cm Visual Analog Scale (VAS) or an Oswestry Disability
Index (ODI) of at least 30%, and an M1, mixed M1/2 or M2 type
change on MRI performed within six months at most prior to
enrolment.
[0285] Patients were excluded from the study if they had renal
impairment with reduced creatinine clearance defined as an
estimated glomerular filtration rate (eGFR) below 40 ml/min,
hypocalcemia, known hypersensitivity to zoledronic acid or other
bisphosphonates or ingredients of the infusion product, the
presence of red flags, nerve root entrapment or willingness for
early retirement. Premenopausal women of childbearing potential
were also excluded. Blood samples were taken prior to the infusion
to assess the serum concentration of calcium and creatinine. The
clinical examination included medical history and clinical
assessment of lumbar flexibility, tendon signs, and motor and
sensory testing.
[0286] After confirmation of eligibility patients were randomized
to receive a single intravenous infusion of 5 mg zoledronic acid
(n=20) or 100 ml saline as placebo (n=20) over a 15-minute period.
Information on use of the concomitant medication and hospital
admissions were recorded. Blood samples were taken for the
assessment of safety, inflammatory mediators and markers of bone
turnover at baseline, one month and one year.
[0287] Clinical assessments were performed 14 days before enrolment
(screening visit), and follow-up visits at one month and one year
after the infusion. The primary outcome was the change in the
intensity of LBP on VAS. Secondary outcomes included leg pain
intensity, ODI, health-related quality of life assessed with
RAND-36, patient-reported sick leaves and lumbar flexibility. These
outcome measures were assessed at baseline and at each follow-up.
Lumbar flexibility was evaluated using the fingers-to-floor and
trunk side bending measures (in cm). The percentage of patients
undergoing a 20% relative improvement, the proportion of patients
reaching a VAS score of 40 or less in the primary outcome, and
patient acceptable symptom state (PASS) were also assessed. Pain
medication use was inquired about during the follow-up visits.
Results
[0288] Zoledronic acid treatment resulted in a greater improvement
in LBP intensity at one month as compared to placebo treatment.
Furthermore, the patients receiving zoledronic acid reported NSAID
use at one year significantly less often than those in the placebo
group. Overall, the improvements in most of the evaluated
parameters were greater in the zoledronic acid group throughout the
follow-up period.
[0289] The clinical characteristics of study participants at
baseline are displayed in Table 6. The mean LBP duration was 293
days, initial LBP intensity on VAS 6.7, leg pain on VAS 2.9 and the
ODI score was 32%. Altogether 19 patients in the ZA group and 18 in
the placebo group had a M1/2 lesion. Modic changes were most
commonly (70%) situated at L4/5 or L5/S1. The zoledronic acid and
placebo groups were similar as regards the demographic and
background characteristics of all patients at baseline (Table
6).
[0290] The mean difference (MD) between the treatment groups in the
primary outcome, intensity of LBP, significantly favored zoledronic
acid at one month (MD 1.4; 95% CI 0.01 to 2.9) while at one year no
significant difference was observed (MD 0.7; 95% CI -1.0 to 2.4;
Table 7). The proportion of patients with at least 20% improvement
in intensity of LBP and PASS both favored the zoledronic acid
treatment at one month: zoledronic acid 55% vs. placebo 25%
(p=0.105) and zoledronic acid 50% vs. placebo 20% (p=0.096),
respectively.
[0291] For the patients who were treated with zoledronic acid, the
reduction in pain intensity was greater in those with greater
baseline pain intensity as shown in Table 9. The mean reduction in
pain from baseline was 3.4 for patients with baseline pain
intensity.gtoreq.7, as compared to a reduction of only 0.1 for
patients with a baseline pain intensity<6.
[0292] Of the secondary outcomes, the improvement in ODI, favored
zoledronic acid at 1 month, the adjusted between-group difference
being 6.0% (95% CI -0.6 to 13), but not at one year (Table 7).
Similarly, side bending (to right and left) favored the zoledronic
acid treatment at one month but not at one year (Table 7). Changes
in total RAND-36, and in the physical and mental components of
RAND-36 are shown in Table 8.
[0293] At baseline, there were no differences in self-reported use
of non-steroidal anti-inflammatory drugs (NSAIDs) between the
treatment groups, whereas at one year, only 20% of patients in the
ZA group used NSAIDs versus 60% in the placebo group.
TABLE-US-00009 TABLE 6 Baseline characteristics of study population
according to treatment group Zoledronic Acid Pacebo Characteristics
n = 20 n = 20 Sex, n (%) men 15 (75) 11 (55) Age, mean (SD) years
49 (9.3) 51 (7.3) Smoking, n (%) regular smokers* 5 (25) 6 (30)
BMI, mean (SD) kg/m 26 (3.3) 27 (3.2) Workload, n (%) Sedentary
work with limited walking 4 (20) 4 (22) Fairly light work with
considerable walking but 4 (20) 3 (17) no lifting or carrying heavy
objects Fairly strenuous work with walking and lifting 8 (40) 6
(33) heaving objects or climbing stairs or uphill Very strenuous
work with lifting or carrying 4 (20) 5 (28) heaving objects such as
shoveling, digging, or hammering Type of worst MC-lesion**, n Type
I 1 1 Type I/II 19 18 Type II 0 1 MC at two or more levels, n (%) 7
(3.5) 4 (20) Levels of MC, n L2/3 4 0 L3/4 3 5 L4/5 6 5 L5/S1 7 10
Duration of LBP, median (IQ range) days 330 (200, 365) 315 (270,
365) Intensity of LBP, mean (SD)*** 6.6 (1.4) 6.8 (1.6) Duration of
leg pain, median (IQ range) days 50 (0, 100) 36 (0, 160) Intensity
of leg pain, mean (SD)*** 3.0 (3.1) 2.9 (2.3) Oswestry Disability
Index, %, Mean (SD) 30 (11) 35 (10) Duration of sick leave during
the past year, 14 (0, 48) 18 (1, 181) median (IQ range) days
RAND-36, mean (SD) 50 (8) 50 (7) RAND-36 physical component, mean
(SD) 51 (8) 49 (8) RAND-36 mental component, mean (SD) 51 (8) 49
(9) BMI = Body Mass Index, MC = Modic Change, LBP = low back pain,
SD = standard deviation, IQ = inter-quartile. *Smoking at least one
cigarette per day. **If different types of MC at two or more
levels, classification is based on the assumed severity of the
type, i.e., Type I > mixed Type I/II > Type II. ***Assessed
using a 10 cm Visual Analogue Scale (VAS).
TABLE-US-00010 TABLE 7 Low back symptoms and lumbar flexibility at
baseline, one month and 12 months according to treatment group and
between group comparisons of difference from baseline to one month
and 12 months Mean (SD) original Unadjusted Adjusted values Mean
(SD) analyses analyses ZA Placebo change Difference Difference n =
20 n = 20 ZA Placebo (95% CI) P (95% CI) P* Intensity of LBP
Baseline 6.6 (1.4) 6.8 (1.6) 1 mo. 4.3 (2.3) 5.8 (2.2) -2.2 -0.9
1.3 0.097 1.4 0.049 (2.7) (2.1) (-0.2 to (0.01 to 2.8) 2.9) 12 mos.
3.8 (2.5) 4.6 (2.9) -2.8 -2.2 0.6 0.474 0.7 0.387 (2.9) (2.5) (-1.1
to (-1.0 to 2.4) 2.4) Intensity of leg pain.sup.a Baseline 3.0
(3.1) 2.9 (2.3) 1 mo. 2.0 (2.3) 3.0 (2.4) -0.6 0.1 0.8 0.367 0.8
0.237 (2.4) (2.6) (-0.9 to (-0.6 to 2.4) 2.2) 12 mos. 2.1 (2.8) 2.7
(2.6) -0.9 -0.3 0.6 0.573 0.5 0.573 (3.4) (3.0) (-1.5 to (-1.3 to
2.7) 2.2) Oswestry disability index, % Baseline 30 (11) 35 (10) 1
mo. 24 (10) 33 (13) -5.9 -1.7 4.3 0.212 6.0 0.071 (11) (9.7) (-2.5
to (-0.6 to 11) 13) 12 mos. 25 (13) 33 (15) -5.0 -1.9 3.1 0.475 5.1
0.231 (15) (12) (-5.6 to (-3.4 to 12) 14) Fingers-to-floor, cm
Baseline 23 (19) 19 (18) 1 mo. 17 (17) 19 (17) -5.1 -0.1 5.0 0.306
3.6 0.403 (20) (8.3) (-4.8 to (-5.0 to 15) 12) 12 mos. 16 (16) 20
(19) -6.3 0.9 7.1 0.215 5.3 0.277 (23) (11) (-4.3 to (-4.5 to 18)
15) Sidebending to right, cm Baseline 14.1 13.8 (4.9) (7.2) 1 mo.
15.7 13.3 1.5 -0.5 -2.0 0.101 -2.0 0.087 (5.9) (6.9) (4.7) (2.2)
(-4.3 to (-4.4 to 0.4) 0.3) 12 mos. 15.7 13.8 1.6 -0.1 -1.6 0.227
-1.7 0.180 (5.6) (6.5) (4.8) (3.5) (-4.3 to (-4.2 to 1.1) 0.8)
Sidebending to left, cm Baseline 15.0 13.3 (5.4) (5.5) 1 mo. 16.1
12.8 1.1 -0.5 -1.5 0.072 -1.7 0.051 (5.3) (5.9) (3.0) (2.2) (-3.2
to (-3.4 to 0.1) 0.0) 12 mos. 16.2 13.7 1.2 0.5 -0.7 0.601 -1.0
0.458 (6.7) (5.7) (5.3) (3.2) (-3.5 to (-3.8 to 2.1) 1.8) SD =
standard deviation, CI = confidence interval, ZA = zoledronic acid,
LBP = low back pain. *ANCOVA: Difference between follow-up and
baseline, treatment effect adjusted for baseline value. .sup.aOne
subject missing at baseline in placebo group and in ZA group, and
one subject at 1 month in ZA group.
TABLE-US-00011 TABLE 8 Health-related quality of life assessed
using RAND-36 at baseline, one month, and 12 months according to
treatment group and between group comparisons of difference from
baseline to one month and 12 months Mean (SD) Unadjusted Adjusted
original values Mean (SD) analyses analyses ZA Placebo change
Difference Difference n = 20 n = 20 ZA Placebo (95% CI) P (95% CI)
P* Total RAND-36 Baseline 50 (8) 50 (7) 1 mo. 51 (8) 49 (8) 0.6
-0.6 1.2 (-3 to 0.530 1.3 (-3 to 0.477 (6.4) (5.0) 5) 5) 12 mos. 51
(8) 49 (9) 1.0 -1.0 2.1 (-3 to 0.378 2.2 (-2 to 0.314 (8.7) (5.9)
7) 7) Physical component Baseline 52 (8) 48 (8) 1 mo. 52 (9) 48 (8)
0.1 -0.1 0.3 (-4 to 0.897 1.3 (-3 to 0.554 (8.6) (5.5) 5) 6) 12
mos. 52 (8) 48 (2) 0.3 -0.3 0.7 (-5 to 0.808 2.1 (-3 to 0.405 (10)
(6.5) 6) 7) Mental component Baseline 49 (9) 51 (8) 1 mo. 50 (9) 50
(9) 1.0 -1.0 2.0 (-2 to 0.286 1.6 (-2 to 0.396 (6.1) (5.6) 6) 5) 12
mos. 51 (9) 49 (9) 1.8 -1.8 3.5 (-2 to 0.167 2.7 (-2 to 0.261 (9.0)
(6.7) 9) 7) SD = standard deviation, CI = confidence interval, ZA =
zoledronic acid. *ANCOVA: Difference between follow-up and
baseline, treatment effect adjusted for baseline value.
TABLE-US-00012 TABLE 9 Pain Reduction in Patients Treated
Zoledronic Acid (cm) VAS Change from Baseline Baseline VAS <6
-0.1 Baseline VAS .gtoreq.6 and <7 -2.3 Baseline VAS .gtoreq.7
-3.4
Example 9
Methods:
[0294] A study was performed to evaluate the efficacy of
bisphosphonates such as oral zoledronic acid in inhibiting immune
responses and pain behavior in a rat fracture model of CRPS.
[0295] The effect of orally administered zoledronic acid was
examined in the rat tibia fracture model of complex regional pain
syndrome (CRPS). CRPS was induced in the rats by fracturing the
right distal tibias of the animals and casting the fractured
hindpaws for 4 weeks, as described in Guo T Z et al. (Pain. 2004;
108: 95-107). This animal model has been shown to replicate the
inciting trauma (such as a fracture, a surgery, a crushing injury,
a cutting injury, a scratch, or a puncture injury), natural
history, signs, symptoms, and pathologic changes observed in human
CRPS patients (Kingery W S et al., Pain. 2003; 104:75-84).
[0296] Starting four weeks after fracture and casting, animals were
orally administered either vehicle (control) or zoledronic acid, a
dose of 21 mg/kg on the first day and 3 mg/kg/day daily thereafter,
or distilled water for 3 weeks (weeks 4-7 post-fracture). Drug was
dissolved in distilled water and administered by gavage. Animals
were fasted for 4 hours before and 2 hours after dosing. At the end
of the 21-day period, casts were removed, and on the following day,
the rats were tested for hindpaw pain, edema, and warmth.
Results
[0297] As illustrated in FIGS. 14-15, treatment with orally
administered zoledronic acid reversed pain and restored weight
bearing as compared to the vehicle treated animals.
[0298] As illustrated in FIG. 14, von Frey pain thresholds for the
right (fracture) hindpaw were reduced by over 100% as compared to
baseline when oral zoledronic acid was administered.
[0299] As illustrated in FIG. 15, reduction in weight bearing, a
postural effect of pain, was significantly higher in the vehicle
treated group as compared to the zoledronic acid treated group.
Weight bearing on the fracture hindlimb was reduced to about 80% of
normal in the vehicle treated group. Zoledronate treatment
significantly restored hindlimb weight bearing as compared to
vehicle treatment (over 90% of normal).
[0300] As can be seen in FIG. 16, orally administering zoledronic
acid four weeks after the fracture resulted in significantly
greater improvement of pain relief as compared to administration at
the time of injury.
Example 10
##STR00012##
[0301] 1,3-Bis(2-methoxy-2-oxoethyl)-1H-imidazol-3-ium chloride
(9)
[0302] Methyl chloroacetate (29.8 mL, 338.6 mmol, 2.0 eq) was added
drop-wise to 1-(trimethylsilyl)-1H-imidazole (8; 25.0 mL, 169.3
mmol). The mixture was heated at 60.degree. C. for 24 hours. The
mixture was cooled to room temperature, washed with Et.sub.2O
(3.times.500 mL) and dried in vacuo yielding 9 (41.97 g, 168.8
mmol, 99.7%) as a white solid.
1,3-Bis(carboxymethyl)1H-imidazol-3-ium chloride (10)
[0303] To 1,3-bis(2-methoxy-2-oxoethyl)-1H-imidazol-3-ium chloride
(9; 41.00 g, 164.88 mmol, 1 eq.) was added 37% aq. HCl (30.03 mL,
362.74 mmol, 2.2 eq.). The mixture was stirred under reflux for 0.5
hour. The mixture was concentrated and the remaining solid was
washed with acetone (2.times.200 mL) and Et.sub.2O (3.times.200
mL). Drying in vacuo gave 10 (31.89 g, 144.55 mmol, 87.7%) as a
white solid.
[0304] Compound 1:
[0305] Compound 10 is reacted with an equimolar amount of
phosphorous acid, followed by an equimolar amount of phosphorous
trichloride, and an excess of water to form Compound 1, which is
precipitated from ethanol.
[0306] Compound 2:
[0307] 1,3-Bis(carboxymethyl)-1H-imidazol-3-ium chloride (10, 2.00
g, 9 mmol, 1.0 eq) and H.sub.3PO.sub.3 (7.37 g, 90 mmol, 10 eq)
were dissolved in toluene (10 mL) and heated to 70.degree. C. The
reaction mixture was stirred at this temperature for 20 min before
PCl.sub.3 (16 mL, 180 mmol, 20 eq) was added within 30 min. The
reaction mixture was then heated to 95.degree. C. and stirred at
this temperature for 2 h. Then, aq. HCl (30 mL, 37% HCl and 5 mL
H.sub.2O) was added. The reaction mixture was heated to 100.degree.
C. and stirred at this temperature for 7 h, then stirred at room
temperature for 2 days and filtered. The filtrate was cooled in an
ice bath and added within 45 min to absolute EtOH (90 mL). The
resulting turbid solution was stirred for 1 h at room temperature
before the solid was filtered off. The filter cake (Compound 2) was
isolated and analyzed by 2D-NMR spectroscopy and mass spectrometry
(m/z=477). The filtrate was concentrated in vacuo to give a
residue. This residue (500 mg) was treated with aq. NaOH (150 mg in
3.5 mL of H.sub.2O) and EtOH (7 mL). After standing overnight the
liquid was decanted and the resulting solid (Na salt of Compound 2)
was obtained and analyzed by NMR and mass spectrometry
(m/z=477).
[0308] The following embodiments are specifically contemplated:
Embodiment 1
[0309] A method of relieving inflammatory pain comprising
administering an oral dosage form containing zoledronic acid to a
mammal in need thereof, wherein the mammal receives a total monthly
dose of zoledronic acid that is about 800 mg/m.sup.2 or less based
upon the body surface area of the mammal.
Embodiment 2
[0310] The method of embodiment 1, wherein the mammal is a human
being that receives a total monthly dose of zoledronic acid that is
about 30 mg/m.sup.2 to about 700 mg/m.sup.2.
Embodiment 3
[0311] The method of embodiment 2, wherein the total monthly dose
is administered in 4 or 5 weekly doses.
Embodiment 4
[0312] The method of embodiment 2, wherein the total monthly dose
is administered in 28 to 31 daily doses.
Embodiment 5
[0313] The method of embodiment 2, wherein the total monthly dose
is administered in 5 to 10 individual doses during the month.
Embodiment 6
[0314] The method of embodiment 1, wherein the mammal is a human
being that receives a total weekly dose of zoledronic acid that is
about 10 mg to about 300 mg.
Embodiment 7
[0315] The method of embodiment 6, wherein the total weekly dose is
a single dose, administered once a week.
Embodiment 8
[0316] The method of embodiment 6, wherein the total weekly dose is
administered in 2 to 7 individual doses during the week.
Embodiment 9
[0317] The method of embodiment 1, wherein the mammal is a human
being that receives a total weekly dose of zoledronic acid that is
about 10 mg to about 150 mg.
Embodiment 10
[0318] The method of any preceding embodiment, wherein the mammal
experiences significant pain relief more than 3 hours after
administration of the dosage form.
Embodiment 11
[0319] The method of embodiment 10, wherein the mammal experiences
significant pain relief during at least a part of a time from about
3 hours to about 24 hours after administration of the dosage
form.
Embodiment 12
[0320] The method of embodiment 10, wherein the mammal experiences
significant pain relief during at least a part of a time from about
3 hours to about 3 weeks after administration of the dosage
form.
Embodiment 13
[0321] A method of relieving inflammatory pain comprising
administering an oral dosage form containing zoledronic acid to a
mammal in need thereof, wherein the oral dosage form contains about
10 mg/m.sup.2 to about 20 mg/m.sup.2 of zoledronic acid based upon
the body surface area of the mammal.
Embodiment 14
[0322] The method of embodiment 13, wherein the oral dosage form
contains about 15 mg/m.sup.2 to about 20 mg/m.sup.2 of zoledronic
acid based upon the body surface area of the mammal.
Embodiment 15
[0323] A method of relieving inflammatory pain comprising orally
administering to a mammal in need thereof, about 300 mg/m.sup.2 to
about 600 mg/m.sup.2 of zoledronic acid per month to the mammal,
based upon the body surface area of the mammal.
Embodiment 16
[0324] The method of embodiment 15, comprising orally administering
about 450 mg/m.sup.2 to about 600 mg/m.sup.2 of zoledronic acid per
month to the mammal, based upon the body surface area of the
mammal.
Embodiment 17
[0325] The method of any preceding embodiment, wherein the mammal
is not suffering from bone metastasis.
Embodiment 18
[0326] The method of any preceding embodiment, wherein the mammal
is not suffering from cancer.
Embodiment 19
[0327] The method of any preceding embodiment, wherein the
zoledronic acid is administered as a salt of a dianion of
zoledronic acid.
Embodiment 20
[0328] A method of relieving pain associated with an arthritis
comprising administering an oral dosage form containing zoledronic
acid to a human being in need thereof.
Embodiment 21
[0329] The method of embodiment 20, wherein the human being
receives a total monthly dose of zoledronic acid that is about 40
mg to about 2000 mg.
Embodiment 22
[0330] The method of embodiment 21, wherein the total monthly dose
is administered in 4 or 5 weekly doses.
Embodiment 23
[0331] The method of embodiment 21, wherein the total monthly dose
is administered in 28 to 31 daily doses.
Embodiment 24
[0332] The method of embodiment 21, wherein the total monthly dose
is administered in 5 to 10 individual doses during the month.
Embodiment 25
[0333] The method of embodiment 20, wherein the human being
receives a total weekly dose of zoledronic acid that is about 100
mg to about 300 mg.
Embodiment 26
[0334] The method of embodiment 25, wherein the total weekly dose
is a single dose, administered once a week.
Embodiment 27
[0335] The method of embodiment 25, wherein the total weekly dose
is administered in 2 to 7 individual doses during the week.
Embodiment 28
[0336] The method of embodiment 20, wherein the human being
receives a total weekly dose of zoledronic acid that is about 10 mg
to about 100 mg.
Embodiment 29
[0337] The method of any of embodiments 20-28, wherein the human
being experiences significant pain relief more than 3 hours after
administration of the dosage form.
Embodiment 30
[0338] The method of embodiment 29, wherein the human being
experiences significant pain relief during at least a part of a
time from about 3 hours to about 24 hours after administration of
the dosage form.
Embodiment 31
[0339] The method of embodiment 29, wherein the human being
experiences significant pain relief during at least a part of a
time from about 3 hours to about 3 weeks after administration of
the dosage form.
Embodiment 32
[0340] The method of any of embodiments 20-31, wherein the dosage
form contains about 10 mg/m.sup.2 to about 20 mg/m.sup.2 of
zoledronic acid based upon the body surface area of the human
being.
Embodiment 33
[0341] The method of embodiment 32, wherein the dosage form
contains about 15 mg/m.sup.2 to about 20 mg/m.sup.2 of zoledronic
acid based upon the body surface area of the human being.
Embodiment 34
[0342] The method of any of embodiments 20-33, wherein about 50
mg/m.sup.2 to about 200 mg/m.sup.2 of zoledronic acid is orally
administered per month, based upon the body surface area of the
human being.
Embodiment 35
[0343] The method of any of embodiments 20-31, wherein the dosage
form contains about 80 mg/m.sup.2 to about 150 mg/m.sup.2 of
zoledronic acid based upon the body surface area of the human
being.
Embodiment 36
[0344] The method of embodiment 35, wherein about 300 mg/m.sup.2 to
about 1000 mg/m.sup.2 of zoledronic acid is orally administered per
month, based upon the body surface area of the human being.
Embodiment 37
[0345] The method of any of embodiments 20-36, wherein the human
being is not suffering from bone metastasis.
Embodiment 38
[0346] The method of any of embodiments 20-37, wherein the human
being is not suffering from cancer.
Embodiment 39
[0347] The method of any preceding embodiment, wherein the
zoledronic acid is in the disodium salt form.
Embodiment 40
[0348] An oral dosage form comprising zoledronic acid, wherein the
oral bioavailability of zoledronic acid in the dosage form is about
0.01% to about 4%.
Embodiment 41
[0349] The oral dosage form of embodiment 40, wherein the oral
dosage form contains about 10 mg to about 300 mg of zoledronic
acid.
Embodiment 42
[0350] The oral dosage form of embodiment 40, wherein the oral
dosage form contains about 10 mg to about 50 mg of zoledronic
acid.
Embodiment 43
[0351] The oral dosage form of any of embodiments 40-42, wherein
the oral bioavailability of zoledronic acid in the dosage form is
about 0.1% to about 2%.
Embodiment 44
[0352] A pharmaceutical product comprising more than one unit of an
oral dosage form of embodiment 40.
Embodiment 45
[0353] The pharmaceutical product of embodiment 44, wherein each
unit of the oral dosage form contains about 1 mg to about 50 mg of
zoledronic acid.
Embodiment 46
[0354] The pharmaceutical product of embodiment 45, comprising 28,
29, 30, or 31 units of the oral dosage form, for a total of about
28 mg to about 1600 mg of zoledronic acid to be administered in
about 1 month.
Embodiment 47
[0355] The pharmaceutical product of embodiment 45, comprising 85
to 95 units of the oral dosage form, for a total of about 85 mg to
about 4800 mg of zoledronic acid to be administered in about 3
months.
Embodiment 48
[0356] The pharmaceutical product of embodiment 45, comprising 170
to 200 units of the oral dosage form, for a total of about 170 mg
to about 10,000 mg of zoledronic acid to be administered in about 6
months.
Embodiment 49
[0357] The pharmaceutical product of embodiment 45, comprising 350
to 380 units of the oral dosage form, for a total of about 350 mg
to about 19,000 mg of zoledronic acid to be administered in about 1
year.
Embodiment 50
[0358] The pharmaceutical product of embodiment 44, wherein each
unit of the oral dosage form contains about 10 mg to about 300
mg.
Embodiment 51
[0359] The pharmaceutical product of embodiment 50, comprising 4 or
5 units of the oral dosage form, for a total of about 40 mg to
about 1500 mg of zoledronic acid to be administered within a period
of about 1 month.
Embodiment 52
[0360] The pharmaceutical product of embodiment 50, comprising 8 or
9 units of the oral dosage form, for a total of about 80 mg to
about 2700 mg of zoledronic acid to be administered in about 2
months.
Embodiment 53
[0361] The pharmaceutical product of embodiment 50, comprising 12,
13 or 14 units of the oral dosage form, for a total of about 120 mg
to about 4200 mg of zoledronic acid to be administered in about 3
months.
Embodiment 54
[0362] The pharmaceutical product of embodiment 50, comprising 22
to 30 units of the oral dosage form, for a total of about 220 mg to
about 9000 mg of zoledronic acid to be administered in about 6
months.
Embodiment 55
[0363] The pharmaceutical product of embodiment 50, comprising 45
to 60 units of the oral dosage form, for a total of about 450 mg to
about 18000 mg of zoledronic acid to be administered in about 1
year.
Embodiment 56
[0364] The pharmaceutical product of embodiment 44, comprising 1 to
10 units of the oral dosage form, wherein the product contains
about 200 mg to about 2000 mg of zoledronic acid.
Embodiment 57
[0365] The oral dosage form of any preceding embodiment, wherein
the zoledronic acid is in the form of a sodium salt.
Embodiment 58
[0366] The oral dosage form of any preceding embodiment, wherein
the zoledronic acid is in a form that has an aqueous solubility
greater than 1% (w/v).
Embodiment 59
[0367] The oral dosage form of any preceding embodiment, wherein
the zoledronic acid is in a form that has an aqueous solubility of
about 5% (w/v) to about 50% (w/v).
Embodiment 60
[0368] An oral dosage form comprising zoledronic acid and an
excipient, wherein the zoledronic acid is in a form that has an
aqueous solubility greater than 1% (w/v).
Embodiment 61
[0369] The oral dosage form of embodiment 60, wherein the
zoledronic acid is in a form that has an aqueous solubility of
about 5% (w/v) to about 50% (w/v).
Embodiment 62
[0370] A method of treating complex regional pain syndrome
comprising administering an oral dosage form containing zoledronic
acid to a mammal in need thereof.
Embodiment 63
[0371] The method of embodiment 62, wherein the mammal is a human
being that receives an amount of zoledronic acid that is about 30
mg/m.sup.2 to about 700 mg/m.sup.2 in a period of one month or
less.
Embodiment 64
[0372] The method of embodiment 63, wherein 4 or 5 weekly doses are
administered in a period of one month or less.
Embodiment 65
[0373] The method of embodiment 63, wherein 28 to 31 daily doses
are administered in a period of one month or less.
Embodiment 66
[0374] The method of embodiment 63, wherein 5 to 10 individual
doses are administered during a period of one month or less.
Embodiment 67
[0375] The method of embodiment 63, wherein about 30 mg/m.sup.2 to
about 700 mg/m.sup.2 of zoledronic acid is administered during only
one month.
Embodiment 68
[0376] The method of embodiment 63, wherein about 30 mg/m.sup.2 to
about 700 mg/m.sup.2 of zoledronic acid is administered in a period
of one month or less for 2 or more consecutive months.
Embodiment 69
[0377] The method of embodiment 62, wherein the mammal receives
about 10 mg/m.sup.2 to about 30 mg/m.sup.2 of zoledronic acid
daily.
Embodiment 70
[0378] The method of embodiment 62, wherein the mammal is a human
being that receives a total weekly dose of zoledronic acid that is
about 10 mg to about 300 mg.
Embodiment 71
[0379] The method of embodiment 70, wherein the total weekly dose
is a single dose, administered once a week.
Embodiment 72
[0380] The method of embodiment 70, wherein the total weekly dose
is administered in 2 to 7 individual doses during the week.
Embodiment 73
[0381] The method of any of embodiments 62-72, wherein the complex
regional pain syndrome is complex regional pain syndrome type
I.
Embodiment 74
[0382] The method of any of embodiments 62-72, wherein the complex
regional pain syndrome is complex regional pain syndrome type
II.
Embodiment 75
[0383] The method of any preceding embodiment, wherein the
zoledronic acid is in a salt form.
Embodiment 76
[0384] The method of any of embodiments 62-75, wherein the dosage
form contains about 10 mg/m.sup.2 to about 20 mg/m.sup.2 of
zoledronic acid based upon the body surface area of the mammal.
Embodiment 77
[0385] The method of embodiment 76, wherein the dosage form
contains about 15 mg/m.sup.2 to about 20 mg/m.sup.2 of zoledronic
acid based upon the body surface area of the mammal.
Embodiment 78
[0386] A method of treating complex regional pain syndrome,
comprising administering pamidronic acid to a human being in need
thereof.
Embodiment 79
[0387] A method of treating complex regional pain syndrome,
comprising administering neridronic acid to a human being in need
thereof.
Embodiment 80
[0388] A method of treating complex regional pain syndrome,
comprising administering olpadronic acid to a human being in need
thereof.
Embodiment 81
[0389] A method of treating complex regional pain syndrome,
comprising administering alendronic acid to a human being in need
thereof.
Embodiment 82
[0390] A method of treating complex regional pain syndrome,
comprising administering incadronic acid to a human being in need
thereof.
Embodiment 83
[0391] A method of treating complex regional pain syndrome,
comprising administering ibandronic acid to a human being in need
thereof.
Embodiment 84
[0392] A method of treating complex regional pain syndrome,
comprising administering risedronic acid to a human being in need
thereof.
Embodiment 85
[0393] A method of treating pain, comprising administering
pamidronic acid to a human being in need thereof.
Embodiment 86
[0394] A method of treating pain, comprising administering
neridronic acid to a human being in need thereof.
Embodiment 87
[0395] A method of treating pain, comprising administering
olpadronic acid to a human being in need thereof.
Embodiment 88
[0396] A method of treating pain, comprising administering
alendronic acid to a human being in need thereof.
Embodiment 89
[0397] A method of treating pain, comprising administering
incadronic acid to a human being in need thereof.
Embodiment 90
[0398] A method of treating pain, comprising administering
ibandronic acid to a human being in need thereof.
Embodiment 91
[0399] A method of treating pain, comprising administering
risedronic acid to a human being in need thereof.
Embodiment 92
[0400] A method of treating arthritis pain, comprising
administering pamidronic acid to a human being in need thereof.
Embodiment 93
[0401] A method of treating arthritis pain, comprising
administering neridronic acid to a human being in need thereof.
Embodiment 94
[0402] A method of treating arthritis pain, comprising
administering olpadronic acid to a human being in need thereof.
Embodiment 95
[0403] A method of treating arthritis pain, comprising
administering alendronic acid to a human being in need thereof.
Embodiment 96
[0404] A method of treating arthritis pain, comprising
administering incadronic acid to a human being in need thereof.
Embodiment 97
[0405] A method of treating arthritis pain, comprising
administering ibandronic acid to a human being in need thereof.
Embodiment 98
[0406] A method of treating arthritis pain, comprising
administering risedronic acid to a human being in need thereof.
Embodiment 99
[0407] A method of treating inflammatory pain, comprising
administering pamidronic acid to a human being in need thereof.
Embodiment 100
[0408] A method of treating inflammatory pain, comprising
administering neridronic acid to a human being in need thereof.
Embodiment 101
[0409] A method of treating inflammatory pain, comprising
administering olpadronic acid to a human being in need thereof.
Embodiment 102
[0410] A method of treating inflammatory pain, comprising
administering alendronic acid to a human being in need thereof.
Embodiment 103
[0411] A method of treating inflammatory pain, comprising
administering incadronic acid to a human being in need thereof.
Embodiment 104
[0412] A method of treating inflammatory pain, comprising
administering ibandronic acid to a human being in need thereof.
Embodiment 105
[0413] A method of treating inflammatory pain, comprising
administering risedronic acid to a human being in need thereof.
Embodiment 106
[0414] A method of treating complex regional pain syndrome,
comprising administering etidronic acid to a human being in need
thereof.
Embodiment 107
[0415] A method of treating pain, comprising administering
etidronic acid to a human being in need thereof.
Embodiment 108
[0416] A method of treating arthritis pain, comprising
administering etidronic acid to a human being in need thereof.
Embodiment 109
[0417] A method of treating inflammatory pain, comprising
administering etidronic acid to a human being in need thereof.
Embodiment 110
[0418] A method of treating complex regional pain syndrome,
comprising administering clodronic acid to a human being in need
thereof.
Embodiment 111
[0419] A method of treating pain, comprising administering
clodronic acid to a human being in need thereof.
Embodiment 112
[0420] A method of treating arthritis pain, comprising
administering clodronic acid to a human being in need thereof.
Embodiment 113
[0421] A method of treating inflammatory pain, comprising
administering clodronic acid to a human being in need thereof.
Embodiment 114
[0422] A method of treating complex regional pain syndrome,
comprising administering tiludronic acid to a human being in need
thereof.
Embodiment 115
[0423] A method of treating pain, comprising administering
tiludronic acid to a human being in need thereof.
Embodiment 116
[0424] A method of treating arthritis pain, comprising
administering tiludronic acid to a human being in need thereof.
Embodiment 117
[0425] A method of treating inflammatory pain, comprising
administering tiludronic acid to a human being in need thereof.
Embodiment 118
[0426] The method of any of embodiments 78-117, wherein the active
compound is orally administered.
Embodiment 119
[0427] The method of any of embodiments 78-117, wherein the active
compound is parenterally administered.
Embodiment 120
[0428] A method of enhancing the oral bioavailability of zoledronic
acid comprising orally administering a dosage form containing
zoledronic acid in the disodium salt form.
Embodiment 121
[0429] The method of embodiment 120, wherein the zoledronic acid in
the disodium salt form provides an enhancement to bioavailability,
as compared to zoledronic acid in the diacid form, which adds to
any enhancement to bioavailability provided by any
bioavailability-enhancing agents in the dosage form.
Embodiment 122
[0430] The method of embodiment 120, wherein the dosage form is
substantially free of bioavailability-enhancing agents.
Embodiment 123
[0431] The method of embodiment 120, wherein the zoledronic acid in
the disodium salt form is administered to a mammal in an amount
that provides an area under the plasma concentration curve of
zoledronic acid of about 4 ngh/mL to about 2000 ngh/mL to the
mammal each time the zoledronic acid in the disodium salt is
administered.
Embodiment 124
[0432] The method of embodiment 123, wherein the zoledronic acid in
the disodium salt form is administered at an interval of about 3 to
about 4 weeks in an amount that provides an area under the plasma
concentration curve of zoledronic acid of about 100 ngh/mL to about
2000 ngh/mL to the mammal each time the zoledronic acid in the
disodium salt form is administered.
Embodiment 125
[0433] The method of embodiment 123, wherein the zoledronic acid in
the disodium salt form is administered weekly, or 3 to 5 times in a
month, in an amount that provides an area under the plasma
concentration curve of zoledronic acid of about 20 ngh/mL to about
700 ngh/mL to the mammal each time the zoledronic acid in the
disodium salt form is administered.
Embodiment 126
[0434] The method of embodiment 123, wherein the zoledronic acid in
the disodium salt form is administered daily in an amount that
provides an area under the plasma concentration curve of zoledronic
acid of about 4 ngh/mL to about 100 ngh/mL to the mammal each time
the zoledronic acid in the disodium salt form is administered.
Embodiment 127
[0435] The method of embodiment 120, wherein the dosage form is a
solid.
Embodiment 128
[0436] The method of embodiment 120, 121, 122, 123, 124, 125, 126,
or 127, wherein the bioavailability of zoledronic acid is improved
by at least about 20% as compared to administration of zoledronic
acid in the diacid form.
Embodiment 129
[0437] The method of embodiment 120, 121, 122, 123, 124, 125, 126,
127, or 128, further comprising administering, on a molar basis,
less of the zoledronic acid in the disodium salt form than would be
administered of zoledronic acid in the diacid form in order to
achieve the same plasma levels of zoledronic acid.
Embodiment 130
[0438] The method of embodiment 129, wherein at least about 10 mole
% less of the disodium salt form is administered as compared the
amount of zoledronic acid in the diacid form that would be
administered in order to achieve the same plasma levels of
zoledronic acid.
Embodiment 131
[0439] The method of embodiment 129, wherein the disodium salt form
is administered in an amount, on a molar basis, that has a value of
about 0.8n.sub.d to about 1.2n.sub.d, wherein:
n.sub.d=(b.sub.a/b.sub.d)(n.sub.a)
wherein b.sub.a is the bioavailability of the diacid form, b.sub.d
is the bioavailability of the disodium salt form, and n.sub.a is
the number of moles of zoledronic acid in the diacid form that
would be administered in order to achieve the same plasma levels of
zoledronic acid.
Embodiment 132
[0440] The method of embodiment 131, wherein the disodium salt is
administered in an amount that has a value of about n.sub.d.
Embodiment 133
[0441] The method of any of embodiments 120-132, wherein the
zoledronic acid is used to treat an inflammatory condition.
Embodiment 134
[0442] The method of embodiment 133, wherein the zoledronic acid is
used to treat arthritis.
Embodiment 135
[0443] The method of embodiment 133, wherein the zoledronic acid is
used to treat complex regional pain syndrome.
Embodiment 136
[0444] The method of any of embodiments 1-39, 62-77, and 120-135,
wherein:
[0445] a first oral dosage form is administered; and
[0446] a second oral dosage form is administered;
[0447] wherein, with respect to the first oral dosage form, the
second oral dosage form is administered at 10.times.T.sub.max or
greater, wherein T.sub.max is the time of maximum plasma
concentration for the first oral dosage form.
Embodiment 137
[0448] A dosage form comprising zoledronic acid in the disodium
salt form, wherein the bioavailability, in a mammal, of zoledronic
acid in the disodium salt form is greater than the bioavailability
of zoledronic acid in the diacid form would be in the same dosage
form.
Embodiment 138
[0449] A dosage form comprising zoledronic acid in the disodium
salt form, wherein the dosage form contains an amount of zoledronic
acid in the disodium salt form that provides an area under the
plasma concentration curve of zoledronic acid of about 4 ngh/mL to
about 2000 ngh/mL to a human being to which the dosage form is
administered.
Embodiment 139
[0450] The dosage form of embodiment 138, wherein the dosage form
contains an amount of zoledronic acid in the disodium salt form
that provides an area under the plasma concentration curve of
zoledronic acid of about 100 ngh/mL to about 2000 ngh/mL to a human
being to which the dosage form is administered.
Embodiment 140
[0451] The dosage form of embodiment 138, wherein the dosage form
contains an amount of zoledronic acid in the disodium salt form
that provides an area under the plasma concentration curve of
zoledronic acid of about 20 ngh/mL to about 700 ngh/mL to a human
being to which the dosage form is administered.
Embodiment 141
[0452] The dosage form of embodiment 138, wherein the dosage form
contains an amount of zoledronic acid in the disodium salt form
that provides an area under the plasma concentration curve of
zoledronic acid of about 4 ngh/mL to about 100 ngh/mL to a human
being to which the dosage form is administered.
Embodiment 142
[0453] A dosage form comprising zoledronic acid in the disodium
salt form,
[0454] wherein the disodium salt form is present in a lower molar
amount than would be present if the zoledronic acid were in the
diacid form; and
[0455] wherein the zoledronic acid in the disodium salt form has an
improved bioavailability as compared to the zoledronic acid in the
diacid form to the extent that the lower molar amount of the
disodium salt in the dosage form does not reduce the amount of
zoledronic acid delivered to the plasma of a mammal.
Embodiment 143
[0456] The dosage form of embodiment 137, 138, 139, 140, 141, or
142, wherein the dosage form is a solid.
Embodiment 144
[0457] The dosage form of embodiment 142 or 143, wherein the
bioavailability of zoledronic acid in the disodium salt form is
improved by at least about 10% as compared to an otherwise
identical dosage form containing zoledronic acid in the diacid
form.
Embodiment 145
[0458] The dosage form of embodiment 142, 143, or 144, containing
at least about 20 mole % less of the disodium salt form as compared
to the amount of the zoledronic acid in the diacid form that would
be present if the zoledronic acid were in the diacid form.
Embodiment 146
[0459] The dosage form of embodiment 142, wherein the disodium salt
form is present in an amount, on a molar basis, that has a value of
about 0.9nd to about 1.1nd, wherein:
n.sub.d=(b.sub.a/b.sub.d)(n.sub.a)
[0460] wherein b.sub.a is the bioavailability of the diacid form,
b.sub.d is the bioavailability of the disodium salt form, and
n.sub.a is the number of moles of the diacid form that would be
present if the zoledronic acid were in the diacid form.
Embodiment 147
[0461] The dosage form of embodiment 146, wherein the disodium salt
is administered in an amount that has a value of about n.sub.d.
Embodiment 148
[0462] The method of any of embodiments 1-39, 62-77, and 120-136,
wherein:
[0463] only a single oral dosage form is administered; or
[0464] a first oral dosage form is administered, and a second oral
dosage form is administered after the first oral dosage form,
wherein the second oral dosage form is administered before the
maximum pain relieving effect of the first oral dosage form is
achieved, or the second oral dosage form is administered before an
observable pain relieving effect is achieved.
Embodiment 149
[0465] The method of embodiment 148, wherein the second oral dosage
form is administered before an observable pain relieving effect is
achieved.
Embodiment 150
[0466] The method of any of embodiments 1-39, 62-77, and 120-132,
wherein a first dosage form is administered, followed by
administration of a second dosage form, wherein the second dosage
form is administered after the maximum pain relieving effect of the
first oral dosage form is achieved, and the second oral dosage form
is administered while a pain relieving effect from the first oral
dosage form is observable.
Embodiment 151
[0467] The method of embodiment 148, 149, or 150, wherein the
second oral dosage form is administered about 24 hours to about 28
days after the first oral dosage form is administered.
Embodiment 152
[0468] The method of any of embodiments 20-39, wherein the human
being is about 30 years old to about 75 years old.
Embodiment 153
[0469] The method of any of embodiments 20-39, wherein the human
being is about 1 year old to about 16 years old.
Embodiment 154
[0470] The method of any of embodiments 20-39, wherein the human
being is about 80 years old to about 95 years old.
Embodiment 155
[0471] The method of any of embodiments 20-39, wherein the human
being has suffered from the arthritis for at least 2 months.
Embodiment 156
[0472] The method of any of embodiments 20-39, wherein the
arthritis affects, a knee, an elbow, a wrist, a shoulder, or a
hip.
Embodiment 157
[0473] The method of any of embodiments 1-44, 62-133, and 144-156,
wherein the mammal or human being to which the zoledronic acid is
administered does not eat food or drink beverage for at least 1
hour before the zoledronic acid is administered.
Embodiment 158
[0474] The method of embodiment 157, wherein the mammal or human
being to which the zoledronic acid is administered does not eat
food or drink beverage for at least 2 hours before the zoledronic
acid is administered.
Embodiment 159
[0475] The method of embodiment 158, wherein the mammal or human
being to which the zoledronic acid is administered does not eat
food or drink beverage for at least 4 hours before the zoledronic
acid is administered.
Embodiment 160
[0476] The method of embodiment 159, wherein the mammal or human
being to which the zoledronic acid is administered does not eat
food or drink beverage for at least 6 hours before the zoledronic
acid is administered.
Embodiment 161
[0477] The method of any of embodiments 157-160, wherein the mammal
or human being to which the zoledronic acid is administered does
not eat food or drink beverage for at least 30 minutes after the
zoledronic acid is administered.
Embodiment 162
[0478] The method of embodiment 161, wherein the mammal or human
being to which the zoledronic acid is administered does not eat
food or drink beverage for at least 1 hour after the zoledronic
acid is administered.
Embodiment 163
[0479] The method of embodiment 161, where in the mammal or human
being to which the zoledronic acid is administered does not eat
food or drink beverage for at least 2 hours after the zoledronic
acid is administered.
Embodiment 164
[0480] The method, dosage form, or product, of any preceding
embodiment, wherein the zoledronic acid in the oral dosage form has
a 24 hour sustained plasma level factor of about 1 or higher.
Embodiment 165
[0481] The method, dosage form, or product, of any preceding
embodiment, wherein the zoledronic acid in the oral dosage form has
a 24 hour sustained plasma level factor that is higher than that of
intravenously administered zoledronic acid.
Embodiment 166
[0482] The method, dosage form, or product, of any preceding
embodiment, wherein the oral dosage form is a solid that has a
hardness of about 5 kPa to about 20 kPa.
Embodiment 167
[0483] A method of treating bone marrow lesions comprising:
selecting a patient having a bone marrow lesion and OARSI grade 0
of joint space narrowing, and administering an inhibitor of
osteoclast activity to the patient for the treatment of the bone
marrow lesion.
Embodiment 168
[0484] The method of embodiment 167, wherein the inhibitor of
osteoclast activity is administered at least twice.
Embodiment 169
[0485] The method of embodiment 167, wherein the inhibitor of
osteoclast activity is administered about every three months, or
more frequently.
Embodiment 170
[0486] The method of embodiment 167, wherein the inhibitor of
osteoclast activity comprises a nitrogen-containing
bisphosphonate.
Embodiment 171
[0487] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises zoledronic
acid.
Embodiment 172
[0488] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises pamidronic
acid.
Embodiment 173
[0489] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises neridronic
acid.
Embodiment 174
[0490] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises olpadronic
acid.
Embodiment 175
[0491] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises alendronic
acid.
Embodiment 176
[0492] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises incadronic
acid.
Embodiment 177
[0493] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises ibandronic
acid.
Embodiment 178
[0494] The method of any one of embodiments 167-170, wherein the
inhibitor of osteoclast activity is or comprises risedronic
acid.
Embodiment 179
[0495] The method of any one of embodiments 167-178, wherein the
inhibitor of osteoclast activity is administered orally.
Embodiment 180
[0496] The method of any one of embodiments 167-178, wherein the
inhibitor of osteoclast activity is administered intravenously.
Embodiment 181
[0497] The method of any one of embodiments 167-180, wherein the
patient experiences a reduction in bone marrow lesion size that is
at least about 100% greater than a reduction in bone marrow lesion
size achieved with a placebo.
Embodiment 182
[0498] The method of any one of embodiments 167-180, wherein the
patient experiences a reduction in bone marrow lesion size that is
at least about 150% greater than a reduction in bone marrow lesion
size achieved with a placebo.
Embodiment 183
[0499] The method of any one of embodiments 167-182, wherein the
inhibitor of osteoclast activity is administered at least twice
over a period of at least four weeks.
Embodiment 184
[0500] The method of any one of embodiments 167-183, wherein the
inhibitor of osteoclast activity is administered once weekly for a
period of six weeks.
Embodiment 185
[0501] The method of any one of embodiments 167-184, wherein the
inhibitor of osteoclast activity comprises zoledronic acid, and the
weekly dose is between about 25 mg and about 75 mg.
Embodiment 186
[0502] A method of treating knee pain comprising: selecting a
patient having knee pain and OARSI grade 0 of joint space
narrowing, and administering an inhibitor of osteoclast activity to
the patient for the treatment of the knee pain.
Embodiment 187
[0503] The method of embodiment 186, wherein the inhibitor of
osteoclast activity is administered at least twice.
Embodiment 188
[0504] The method of any one of embodiments 186-187, wherein the
inhibitor of osteoclast activity is administered about every three
months, or more frequently.
Embodiment 189
[0505] The method of any one of embodiments 186-188, wherein the
inhibitor of osteoclast activity comprises a nitrogen-containing
bisphosphonate.
Embodiment 190
[0506] The method of any one of embodiments 186-189, wherein the
patient experiences pain relief three months after administration
of the inhibitor of osteoclast activity.
Embodiment 191
[0507] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises zoledronic
acid.
Embodiment 192
[0508] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises pamidronic
acid.
Embodiment 193
[0509] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises neridronic
acid.
Embodiment 194
[0510] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises olpadronic
acid.
Embodiment 195
[0511] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises alendronic
acid.
Embodiment 196
[0512] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises incadronic
acid.
Embodiment 197
[0513] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises ibandronic
acid.
Embodiment 198
[0514] The method of any one of embodiments 186-190, wherein the
inhibitor of osteoclast activity is or comprises risedronic
acid.
Embodiment 199
[0515] The method of any one of embodiments 186-198, wherein the
patient experiences a reduction in pain intensity--when using a 100
mm visual analog scale--of at least about 20.
Embodiment 200
[0516] A method of treating a bone marrow lesion of the knee
comprising: selecting a patient having a bone marrow lesion of the
knee and OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1
of joint space narrowing, and administering an inhibitor of
osteoclast activity to the patient for the treatment of the bone
marrow lesion.
Embodiment 201
[0517] The method of embodiment 200, wherein the inhibitor of
osteoclast activity is administered at least twice.
Embodiment 202
[0518] The method of embodiment 201, wherein the inhibitor of
osteoclast activity is administered about every three months, or
more frequently.
Embodiment 203
[0519] The method of embodiment 200, wherein the inhibitor of
osteoclast activity comprises a nitrogen-containing
bisphosphonate.
Embodiment 204
[0520] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is zoledronic acid.
Embodiment 205
[0521] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is pamidronic acid.
Embodiment 206
[0522] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is neridronic acid.
Embodiment 207
[0523] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is olpadronic acid.
Embodiment 208
[0524] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is minodronic acid.
Embodiment 209
[0525] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is incadronic acid.
Embodiment 210
[0526] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is ibandronic acid.
Embodiment 211
[0527] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is risedronic acid.
Embodiment 212
[0528] The method of embodiment 203, wherein the inhibitor of
osteoclast activity is alendronic acid.
Embodiment 213
[0529] The method of embodiment 200, wherein the inhibitor of
osteoclast activity is administered orally.
Embodiment 214
[0530] The method of embodiment 200, wherein the inhibitor of
osteoclast activity is administered intravenously.
Embodiment 215
[0531] The method of embodiment 200, wherein the patient
experiences a reduction in bone marrow lesion size that is at least
about 15% within about 6 months after the inhibitor of osteoclast
activity is administered to the patient.
Embodiment 216
[0532] The method of embodiment 200, wherein the patient
experiences a reduction in bone marrow lesion size that is at least
about 25% within about 6 months after the inhibitor of osteoclast
activity is administered to the patient.
Embodiment 217
[0533] The method of embodiment 201, wherein the inhibitor of
osteoclast activity is administered at least twice over a period of
at least four weeks.
Embodiment 218
[0534] The method of embodiment 201, wherein the inhibitor of
osteoclast activity is administered once weekly for a period of six
weeks.
Embodiment 219
[0535] The method of embodiment 218, wherein the inhibitor of
osteoclast activity comprises zoledronic acid, and the weekly dose
is between about 25 mg and about 75 mg.
Embodiment 220
[0536] A method of treating knee pain comprising:
[0537] a. selecting a patient having knee pain, and: [0538] i.
OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint
space narrowing, or [0539] ii. pain intensity of 5 or greater
measured using the 0-10 NRS or 5 cm or greater using the 10 cm VAS;
and
[0540] b. administering an inhibitor of osteoclast activity to the
patient.
Embodiment 221
[0541] The method of embodiment 220, comprising selecting a patient
having OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of
joint space narrowing.
Embodiment 222
[0542] The method of embodiment 220 or 221, comprising selecting a
patient having pain intensity of 5 or greater measured using the
0-10 NRS or 5 cm or greater using the 10 cm VAS.
Embodiment 223
[0543] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is administered at least twice.
Embodiment 224
[0544] The method of embodiment 223, wherein the inhibitor of
osteoclast activity is administered about every three months, or
more frequently.
Embodiment 225
[0545] The method of embodiment 220, wherein the inhibitor of
osteoclast activity comprises a nitrogen-containing
bisphosphonate.
Embodiment 226
[0546] The method of embodiment 220, wherein the patient
experiences pain relief within about three months after the
inhibitor of osteoclast activity is administered to the
patient.
Embodiment 227
[0547] The method of embodiment 226, wherein the patient
experiences pain relief at least 24 hours after the inhibitor of
osteoclast activity is administered to the patient.
Embodiment 228
[0548] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is zoledronic acid.
Embodiment 229
[0549] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is minodronic acid.
Embodiment 230
[0550] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is neridronic acid.
Embodiment 231
[0551] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is olpadronic acid.
Embodiment 232
[0552] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is alendronic acid.
Embodiment 233
[0553] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is incadronic acid.
Embodiment 234
[0554] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is ibandronic acid.
Embodiment 235
[0555] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is risedronic acid.
Embodiment 236
[0556] The method of embodiment 220, wherein the patient
experiences a reduction in pain intensity--when using a 100 mm
visual analog scale--of at least about 5.
Embodiment 237
[0557] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is administered at least twice over a period of
at least four weeks.
Embodiment 238
[0558] The method of embodiment 220, wherein the inhibitor of
osteoclast activity is administered once weekly for a period of six
weeks.
Embodiment 239
[0559] The method of embodiment 238, wherein the inhibitor of
osteoclast activity comprises zoledronic acid, and the weekly dose
is between about 25 mg and about 75 mg.
Embodiment 240
[0560] A method of treating moderate to severe knee pain comprising
administering an inhibitor of osteoclast activity to a person
suffering from moderate to severe knee pain.
Embodiment 241
[0561] The method of embodiment 240, wherein the person suffering
from moderate to severe knee pain has a normal joint space in the
knee.
Embodiment 242
[0562] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is administered at least twice.
Embodiment 243
[0563] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is administered about every three months, or
more frequently.
Embodiment 244
[0564] The method of embodiment 240, wherein the inhibitor of
osteoclast activity comprises a nitrogen-containing
bisphosphonate.
Embodiment 245
[0565] The method of embodiment 240, wherein the patient
experiences pain relief within about three months after the
inhibitor of osteoclast activity is administered to the
patient.
Embodiment 246
[0566] The method of embodiment 245, wherein the patient
experiences pain relief at least 24 hours after the inhibitor of
osteoclast activity is administered to the patient.
Embodiment 247
[0567] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is zoledronic acid.
Embodiment 248
[0568] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is minodronic acid.
Embodiment 249
[0569] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is neridronic acid.
Embodiment 250
[0570] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is olpadronic acid.
Embodiment 251
[0571] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is alendronic acid.
Embodiment 252
[0572] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is incadronic acid.
Embodiment 253
[0573] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is ibandronic acid.
Embodiment 254
[0574] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is risedronic acid.
Embodiment 255
[0575] The method of embodiment 240, wherein the patient
experiences a reduction in pain intensity--when using a 100 mm
visual analog scale--of at least about 5.
Embodiment 256
[0576] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is administered at least twice over a period of
at least four weeks.
Embodiment 257
[0577] The method of embodiment 240, wherein the inhibitor of
osteoclast activity is administered once weekly for a period of six
weeks.
Embodiment 258
[0578] The method of embodiment 257, wherein the inhibitor of
osteoclast activity comprises zoledronic acid, and the weekly dose
is between about 25 mg and about 75 mg.
Embodiment 259
[0579] A method of safely delivering zoledronic acid to the blood
of a mammal through repeated oral administration comprising: [0580]
orally administering about 0.4 mg/kg to about 4 mg/kg of zoledronic
acid to the mammal no more frequently than once a day and more
frequently than once a week; or [0581] orally administering about
0.4 mg/kg to about 10 mg/kg to the mammal once a week, or less
frequently.
Embodiment 260
[0582] The method of any preceding embodiment, such as embodiment
259, wherein about 0.5 mg/kg to about 2 mg/kg is orally
administered to the mammal daily.
Embodiment 261
[0583] The method of any preceding embodiment, such as embodiment
260, wherein about 0.6 mg/kg to about 0.9 mg/kg is orally
administered to the mammal daily.
Embodiment 262
[0584] The method of any preceding embodiment, such as embodiment
259, wherein about 0.5 mg/kg to about 2 mg/kg is orally
administered to the mammal weekly.
Embodiment 263
[0585] The method of any preceding embodiment, such as embodiment
263, wherein about 0.6 mg/kg to about 0.9 mg/kg is orally
administered to the mammal weekly.
Embodiment 264
[0586] The method of any preceding embodiment, such as embodiment
259, 260, 261, 262, or 263, wherein zoledronic acid is orally
administered about 3 to about 10 times.
Embodiment 265
[0587] The method of any preceding embodiment, such as embodiment
259, 260, 261, 262, 263, or 264, wherein zoledronic acid is orally
administered in a dosage form comprising more than about 10%
zoledronic acid by weight.
Embodiment 266
[0588] The method of any preceding embodiment, such as embodiment
259, 260, 261, 262, 263, 264, or 265, wherein zoledronic acid is
administered in a manner and amount that results in the mammal
having an AUC.sub.0-24 of zoledronic acid that is about 50 ngh/mL
to about 500 ngh/mL with each administration of zoledronic
acid.
Embodiment 267
[0589] The method of any preceding embodiment, such as embodiment
266, wherein zoledronic acid is administered in a manner and amount
that results in the mammal having an AUC.sub.0-24 of zoledronic
acid that is about 100 ngh/mL to about 500 ngh/mL with each
administration of zoledronic acid.
Embodiment 268
[0590] A method of preparing an oral dosage form that is safe for
repeated administration to a mammal comprising combining zoledronic
acid with an excipient that is pharmaceutically acceptable to the
mammal, wherein the amount of zoledronic acid that is combined with
the excipient is such that zoledronic acid is present in the oral
dosage form in an amount that is 0.4 mg/kg to about 10 mg/kg based
upon the weight of the mammal.
Embodiment 269
[0591] The method of any preceding embodiment, such as embodiment
268, wherein the amount of zoledronic acid that is combined with
the excipient is such that the oral dosage form comprises more than
about 10% zoledronic acid by weight.
Embodiment 270
[0592] The method of any preceding embodiment, such as embodiment
268 or 269, wherein the amount of zoledronic acid that is combined
with the excipient is such that zoledronic acid is present in the
oral dosage form in an amount that is 0.4 mg/kg to about 3 mg/kg
based upon the weight of the mammal.
Embodiment 271
[0593] The method of any preceding embodiment, such as embodiment
270, wherein the amount of zoledronic acid that is combined with
the excipient is such that zoledronic acid is present in the oral
dosage form in an amount that is 0.4 mg/kg to about 1.5 mg/kg based
upon the weight of the mammal.
Embodiment 272
[0594] The method of any preceding embodiment, such as embodiment
270, wherein the amount of zoledronic acid that is combined with
the excipient is such that zoledronic acid is present in the oral
dosage form in an amount that is 0.6 mg/kg to about 0.9 mg/kg based
upon the weight of the mammal.
Embodiment 273
[0595] The method of any preceding embodiment, such as embodiment
268, 269, 270, 271, or 272, wherein the oral dosage form is safe
for once daily administration of the oral dosage form for about 3
to about 10 days.
Embodiment 274
[0596] The method of any preceding embodiment, such as embodiment
268, 269, 270, 271, or 272, wherein the oral dosage form is safe
for once weekly administration of the oral dosage form for about 3
to about 10 weeks.
Embodiment 275
[0597] A method of safely delivering zoledronic acid to the blood
of a mammal through repeated oral administration comprising: [0598]
orally administering about 0.05 mg/kg to about 4 mg/kg of
zoledronic acid to the mammal no more frequently than once a day
and more frequently than once a week; or [0599] orally
administering about 0.1 mg/kg to about 10 mg/kg to the mammal once
a week, or less frequently
[0600] wherein zoledronic acid is orally administered at least 5
times.
Embodiment 276
[0601] The method of any preceding embodiment, such as embodiment
275, wherein zoledronic acid is orally administered about 5 to
about 10 times.
Embodiment 277
[0602] The method of any preceding embodiment, such as embodiment
275 or 276, wherein zoledronic acid is orally administered in a
dosage form comprising more than about 10% zoledronic acid by
weight.
Embodiment 278
[0603] The method of any preceding embodiment, such as embodiment
259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271,
272, 273, 274, 275, 276, or 277, wherein the mammal is a human
being.
Embodiment 279
[0604] The method of any preceding embodiment, such as embodiment
259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271,
272, 273, 274, 275, 276, 277, or 278, wherein about 50 mg to about
350 mg of oral zoledronic acid is administered to the mammal per
month.
Embodiment 280
[0605] An oral dosage form prepared by the method of any preceding
embodiment, such as embodiment 259, 260, 261, 262, 263, 264, 265,
266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, or
279.
Embodiment 281
[0606] An oral dosage form prepared by the method of any preceding
embodiment, wherein an osteoclast inhibitor, including a
bisphosphonate, such as zoledronic acid, neridronic acid, etc., is
in a dosage form containing one of, or a combination of, the
ingredients in the Table E.
Embodiment 282
[0607] A molecular complex comprising zoledronic acid or neridronic
acid in an acid or a salt form.
Embodiment 283
[0608] The molecular complex of Embodiment 282, further comprising
a basic or a salt form of a) an amine, b) an amide, or c)
ammonium.
Embodiment 284
[0609] The molecular complex of Embodiment 283, wherein the
molecular complex comprises ammonia in a salt form.
Embodiment 285
[0610] The molecular complex of Embodiment 283, wherein the amine
is an amino acid.
Embodiment 286
[0611] The molecular complex of Embodiment 285, wherein the amino
acid is a lysine.
Embodiment 287
[0612] The molecular complex of Embodiment 285, wherein the amino
acid is L-lysine.
Embodiment 288
[0613] The molecular complex of Embodiment 285, wherein the amino
acid is D-lysine.
Embodiment 289
[0614] The molecular complex of Embodiment 285, wherein the amino
acid is DL-lysine.
Embodiment 290
[0615] The molecular complex of Embodiment 285, wherein the amino
acid is a glycine.
Embodiment 291
[0616] The molecular complex of Embodiment 285, wherein the amino
acid is L-glycine.
Embodiment 292
[0617] The molecular complex of Embodiment 285, wherein the amino
acid is D-glycine.
Embodiment 293
[0618] The molecular complex of Embodiment 285, wherein the amino
acid is DL-glycine.
Embodiment 294
[0619] The molecular complex of Embodiment 283, wherein the amide
is nicotinamide.
Embodiment 295
[0620] The molecular complex of Embodiment 283, wherein the amine
is adenine.
Embodiment 296
[0621] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is alanine.
Embodiment 297
[0622] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is arginine.
Embodiment 298
[0623] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is asparagine.
Embodiment 299
[0624] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is aspartic acid.
Embodiment 300
[0625] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is cysteine.
Embodiment 301
[0626] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is glutamic acid.
Embodiment 302
[0627] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is glutamine.
Embodiment 303
[0628] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is histidine.
Embodiment 304
[0629] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is isoleucine.
Embodiment 305
[0630] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is leucine.
Embodiment 306
[0631] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is methionine.
Embodiment 307
[0632] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is phenylalanine.
Embodiment 308
[0633] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is proline.
Embodiment 309
[0634] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is serine.
Embodiment 310
[0635] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is threonine.
Embodiment 311
[0636] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is tryptophan.
Embodiment 312
[0637] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is tyrosine.
Embodiment 313
[0638] The molecular complex of Embodiment 285, wherein the amino
acid (including the DL-mixture, a D-enantiomer, or an L-enantiomer)
is valine.
Embodiment 314
[0639] A dosage form comprising the molecular complex of Embodiment
282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,
295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307,
308, 309, 310, 311, 312, or 313.
Embodiment 315
[0640] The dosage form of Embodiment 314, which is an oral dosage
form.
Embodiment 316
[0641] A method of treating pain, a musculoskeletal condition, or a
condition related to bone or joint comprising administering a
dosage form of Embodiment 314 or 315 to a mammal in need
thereof.
Embodiment 317
[0642] The method of Embodiment 316, wherein the mammal is a human
being.
Embodiment 318
[0643] The method of Embodiment 316 or 317, comprising treating
acute pain, central pain, radio-therapy or chemo-therapy associated
neuropathy, ankylosing spondylitis, arthritis, axial
spondyloarthritis, blood cancers, bone fracture, bone metastases
from solid tumors, bone metastasis, breast cancer, cancer, central
multiple sclerosis pain, Charcot's foot, chronic pain, complex
regional pain syndrome, diabetic peripheral neuropathy, erosive
osteoarthritis, excessive bone resorption, fibrous dysplasia, giant
cell tumor of bone, HIV-associated neuropathy, hypercalcemia of
malignancy, inflammatory pain, juvenile rheumatoid arthritis,
leukemias, low back pain, lumbar nerve root compression,
lumbosacral pain, lung cancer, metastatic bone cancer,
monoradiculopathies, multiple myeloma, musculoskeletal pain,
neuropathic arthropaties, neuropathic pain, non-articular
rheumatism, osteoarthritis, osteogenesis imperfecta, osteoporosis,
Paget's disease, Paget's disease of bone, peri-articular disorders,
phantom limb pain, post-herpetic neuralgia, postoperative pain,
post-stroke pain, prostate cancer, rheumatoid arthritis, SAPHO
syndrome, sero-negative (non-rheumatoid) arthropathies, solid
tumors or cancers, spinal cord injury, systemic lupus
erythematosus, transient osteoarthritis of the hip, transient
osteoporosis, transient osteoporosis of the hip, trigeminal
neuralgia, tumor induced hypocalcemia, or vertebral crush
fracture.
Embodiment 319
[0644] The method of Embodiment 316 or 317, comprising treating
arthritis.
Embodiment 320
[0645] The method of Embodiment 319, comprising relieving pain
associated with arthritis.
Embodiment 321
[0646] The method of Embodiment 320, wherein the arthritis affects
a knee, an elbow, a wrist, a shoulder, or a hip.
Embodiment 322
[0647] The method of Embodiment 321, wherein the arthritis affects
a knee.
Embodiment 323
[0648] The method of Embodiment 316 or 317, comprising treating
musculoskeletal pain.
Embodiment 324
[0649] The method of Embodiment 316 or 317, comprising treating a
bone marrow lesion.
Embodiment 325
[0650] The method of Embodiment 324, wherein the mammal is a human
being that experiences a reduction in bone marrow lesion size that
is at least about 15% within about 6 months after the inhibitor of
osteoclast activity is administered to the human being.
Embodiment 326
[0651] The method of Embodiment 324, wherein the mammal is a human
being that experiences a reduction in bone marrow lesion size that
is at least about 25% within about 6 months after the inhibitor of
osteoclast activity is administered to the human being.
Embodiment 327
[0652] The method of Embodiment 324, 325, or 326, wherein the bone
marrow lesion affects a knee.
Embodiment 328
[0653] The method of Embodiment 324, 325, 326, or 327, comprising
treating a bone marrow lesion of the knee by selecting a patient
having a bone marrow lesion of the knee and OARSI Grade 0 or
Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing,
and administering the dosage form to the patient for the treatment
of the bone marrow lesion.
Embodiment 329
[0654] The method of Embodiment 316 or 317, comprising treating
osteoarthritis.
Embodiment 330
[0655] The method of Embodiment 329, wherein the osteoarthritis
affects a knee.
Embodiment 331
[0656] The method of Embodiment 329 or 330, comprising treating an
osteolytic lesion associated with osteoarthritis.
Embodiment 332
[0657] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, or 331, comprising treating
knee pain.
Embodiment 333
[0658] The method of Embodiment 332, comprising treating moderate
to severe knee pain.
Embodiment 334
[0659] The method of Embodiment 332 or 333, wherein the mammal is a
human being that has a normal joint space in the knee.
Embodiment 335
[0660] The method of Embodiment 332, comprising treating knee pain
by: [0661] 1) selecting a patient having knee pain, and: [0662] a.
OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint
space narrowing, or [0663] b. pain intensity of 5 or greater
measured using the 0-10 NRS, or 5 cm or greater using the 10 cm
VAS; and [0664] 2) administering the dosage form to the
patient.
Embodiment 336
[0665] The method of Embodiment 335, comprising selecting a patient
having OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of
joint space narrowing.
Embodiment 337
[0666] The method of Embodiment 335, comprising selecting a patient
having pain intensity of 5 or greater measured using the 0-10 NRS,
or 5 cm or greater using the 10 cm VAS.
Embodiment 338
[0667] The method of Embodiment 335, 336, or 337, wherein the
patient experiences a reduction in pain intensity--when using a 100
mm visual analog scale--of at least about 5 mm.
Embodiment 339
[0668] The method of Embodiment 316 or 317, comprising treating
musculoskeletal pain.
Embodiment 340
[0669] The method of Embodiment 316 or 317, comprising treating
inflammatory pain.
Embodiment 341
[0670] The method of Embodiment 316 or 317, comprising treating
back pain.
Embodiment 342
[0671] The method of Embodiment 341, wherein the back pain
comprises low back pain.
Embodiment 343
[0672] The method of Embodiment 342, wherein the low back pain is
related to a vertebral change.
Embodiment 344
[0673] The method of Embodiment 316 or 317, comprising treating
type 1 Modic changes, or type 1 and type 2 Modic changes.
Embodiment 345
[0674] The method of Embodiment 344, wherein the Modic change is
located at C1/2, C2/3, C3/4, C4/5, C5/6, or C6/7.
Embodiment 346
[0675] The method of Embodiment 344, wherein the Modic change is
located at C7/T1, T1/2, T2/3, T3/4, T4/5, T5/6, T6/7, T7/8, T8/9,
T9/10, T10/11, or T11/12.
Embodiment 347
[0676] The method of Embodiment 344, wherein the Modic change is
located at T12/L1, L1/2, L2/3, L3/4, L4/5, or L5/S1.
Embodiment 348
[0677] The method of Embodiment 316 or 317, comprising treating
pain in an extremity.
Embodiment 349
[0678] The method of Embodiment 316 or 317, comprising treating
joint pain.
Embodiment 350
[0679] The method of Embodiment 316 or 317, comprising treating
muscle pain.
Embodiment 351
[0680] The method of Embodiment 316 or 317, comprising treating
neuropathic pain.
Embodiment 352
[0681] The method of Embodiment 316 or 317, comprising treating
complex regional pain syndrome.
Embodiment 353
[0682] The method of Embodiment 352, wherein the complex regional
pain syndrome is complex regional pain syndrome type I.
Embodiment 354
[0683] The method of Embodiment 352, wherein the complex regional
pain syndrome is complex regional pain syndrome type II.
Embodiment 355
[0684] The method of Embodiment 316 or 317, comprising treating
Paget's disease of bone.
Embodiment 356
[0685] The method of Embodiment 316 or 317, comprising treating
multiple myeloma.
Embodiment 357
[0686] The method of Embodiment 316 or 317, comprising treating
ankylosing spondylitis.
Embodiment 358
[0687] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, or 357, wherein the dosage
form is administered about every three months, or more
frequently.
Embodiment 359
[0688] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, or 358, wherein the
mammal experiences pain relief at least 24 hours after the dosage
form is administered to the mammal.
Embodiment 360
[0689] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, or 359, wherein
the mammal experiences pain relief three months after the dosage
form is administered.
Embodiment 361
[0690] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, or 360,
wherein the human being experiences pain relief that lasts for a
duration of at least 48 hours after administration of the dosage
form.
Embodiment 362
[0691] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, or 361,
wherein the human being receives the dosage form no more often than
once daily.
Embodiment 363
[0692] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, or 361,
wherein there is a period of about 24 hours to about 7 days between
administration of dosage forms.
Embodiment 364
[0693] The method of Embodiment 363, wherein the dosage form is
administered weekly.
Embodiment 365
[0694] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, or 361,
wherein there is a period of about 14 days to about 28 days between
administration of dosage forms.
Embodiment 366
[0695] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, or 361,
wherein there is a period of at least one month between
administration of dosage forms.
Embodiment 367
[0696] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, or 361,
wherein there is a period of about 7 days to about 14 days between
administration of dosage forms.
Embodiment 368
[0697] The method of Embodiment 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,
362, 363, 364, 365, 366, or 367, wherein the compound is
administered more than once.
Embodiment 369
[0698] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity and/or CTX serum
levels.
Embodiment 370
[0699] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
5%.
Embodiment 371
[0700] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
10%.
Embodiment 372
[0701] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
15%.
Embodiment 373
[0702] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
20%.
Embodiment 374
[0703] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
25%.
Embodiment 375
[0704] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
30%.
Embodiment 376
[0705] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
35%.
Embodiment 377
[0706] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
40%.
Embodiment 378
[0707] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
45%.
Embodiment 379
[0708] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
50%.
Embodiment 380
[0709] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
55%.
Embodiment 381
[0710] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
60%.
Embodiment 382
[0711] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at about
60%-70%.
Embodiment 383
[0712] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by about 70%-80%.
Embodiment 384
[0713] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
70%.
Embodiment 385
[0714] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at about 75%.
Embodiment 386
[0715] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by about 80%-90%.
Embodiment 387
[0716] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
80%.
Embodiment 388
[0717] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
85%.
Embodiment 389
[0718] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by about 80%-85%.
Embodiment 390
[0719] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
90%.
Embodiment 391
[0720] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by about 85%-90%.
Embodiment 392
[0721] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by about 85%-95%.
Embodiment 393
[0722] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by about 90%-95%.
Embodiment 394
[0723] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
95%.
Embodiment 395
[0724] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
99%.
Embodiment 396
[0725] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases osteoclast activity by at least about
100%.
Embodiment 397
[0726] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
5%.
Embodiment 398
[0727] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
10%.
Embodiment 399
[0728] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
15%.
Embodiment 400
[0729] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
20%.
Embodiment 401
[0730] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
25%.
Embodiment 402
[0731] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
30%.
Embodiment 403
[0732] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
35%.
Embodiment 404
[0733] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
40%.
Embodiment 405
[0734] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
45%.
Embodiment 406
[0735] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
50%.
Embodiment 407
[0736] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
55%.
Embodiment 408
[0737] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
60%.
Embodiment 409
[0738] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 60%-70%.
Embodiment 410
[0739] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 70%-80%.
Embodiment 411
[0740] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by least about 70%.
Embodiment 412
[0741] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
75%.
Embodiment 413
[0742] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
80%.
Embodiment 414
[0743] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
85%.
Embodiment 415
[0744] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 80%-85%.
Embodiment 416
[0745] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
90%.
Embodiment 417
[0746] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 80%-90%.
Embodiment 418
[0747] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 85%-90%.
Embodiment 419
[0748] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 85%-95%.
Embodiment 420
[0749] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by about 90%-95%.
Embodiment 421
[0750] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
95%.
Embodiment 422
[0751] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
99%.
Embodiment 423
[0752] The method, composition, molecular complex, dosage form, or
product, of any preceding embodiment, wherein zoledronic acid or
neridronic acid decreases CTX serum levels by at least about
100%.
Embodiment 424
[0753] The method, dosage form, or product, of any preceding
embodiment, wherein the zoledronic acid is orally administered in a
manner that results in a 24 hour sustained plasma level factor that
is at least 1.5 times that of 4 mg of zoledronic acid administered
intravenously.
[0754] Unless otherwise indicated, all numbers expressing
quantities of ingredients, properties such as molecular weight,
reaction conditions, and so forth used in the specification and
claims are to be understood in all instances as indicating both the
exact values as shown and as being modified by the term "about."
Accordingly, unless indicated to the contrary, the numerical
parameters set forth in the specification and attached claims are
approximations that may vary depending upon the desired properties
sought to be obtained. At the very least, and not as an attempt to
limit the application of the doctrine of equivalents to the scope
of the claims, each numerical parameter should at least be
construed in light of the number of reported significant digits and
by applying ordinary rounding techniques.
[0755] The terms "a," "an," "the" and similar referents used in the
context of describing the invention (especially in the context of
the following claims) are to be construed to cover both the
singular and the plural, unless otherwise indicated herein or
clearly contradicted by context. All methods described herein can
be performed in any suitable order unless otherwise indicated
herein or otherwise clearly contradicted by context. The use of any
and all examples, or exemplary language (e.g., "such as") provided
herein is intended merely to better illuminate the invention and
does not pose a limitation on the scope of any claim. No language
in the specification should be construed as indicating any
non-claimed element essential to the practice of the invention.
[0756] Groupings of alternative elements or embodiments disclosed
herein are not to be construed as limitations. Each group member
may be referred to and claimed individually or in any combination
with other members of the group or other elements found herein. It
is anticipated that one or more members of a group may be included
in, or deleted from, a group for reasons of convenience and/or
patentability. When any such inclusion or deletion occurs, the
specification is deemed to contain the group as modified thus
fulfilling the written description of all Markush groups used in
the appended claims.
[0757] Certain embodiments are described herein, including the best
mode known to the inventors for carrying out the invention. Of
course, variations on these described embodiments will become
apparent to those of ordinary skill in the art upon reading the
foregoing description. The inventor expects skilled artisans to
employ such variations as appropriate, and the inventors intend for
the invention to be practiced otherwise than specifically described
herein. Accordingly, the claims include all modifications and
equivalents of the subject matter recited in the claims as
permitted by applicable law. Moreover, any combination of the
above-described elements in all possible variations thereof is
contemplated unless otherwise indicated herein or otherwise clearly
contradicted by context.
[0758] In closing, it is to be understood that the embodiments
disclosed herein are illustrative of the principles of the claims.
Other modifications that may be employed are within the scope of
the claims. Thus, by way of example, but not of limitation,
alternative embodiments may be utilized in accordance with the
teachings herein. Accordingly, the claims are not limited to
embodiments precisely as shown and described.
* * * * *