U.S. patent application number 15/334827 was filed with the patent office on 2017-05-04 for thermostable pectinase. The applicant listed for this patent is DONGGUAN APAC BIOTECHNOLOGY CO., LTD.. Invention is credited to Ya-Shan Cheng, Rey-Ting Guo, Jian-Wen Huang, Tsung-Yu Ko, Hui-Lin Lai, Cheng-Yen Lin, Tzu-Hui Wu.
Application Number | 20170121699 15/334827 |
Document ID | / |
Family ID | 58608451 |
Filed Date | 2017-05-04 |
United States Patent Application | 20170121699 |
Kind Code | A1 |
Guo; Rey-Ting ; et al. | May 4, 2017 |
A pectinase having improved thermostability is disclosed. The pectinase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of serine at position 181 with phenylalanine, methionine or leucine.
Inventors: | Guo; Rey-Ting; (Taipei, TW) ; Cheng; Ya-Shan; (Taipei, TW) ; Huang; Jian-Wen; (Taipei, TW) ; Wu; Tzu-Hui; (Taipei, TW) ; Lai; Hui-Lin; (Taipei, TW) ; Lin; Cheng-Yen; (Taipei, TW) ; Ko; Tsung-Yu; (Taipei, TW) | ||||||||||
Applicant: |
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Family ID: | 58608451 | ||||||||||
Appl. No.: | 15/334827 | ||||||||||
Filed: | October 26, 2016 |
Current U.S. Class: | 1/1 |
Current CPC Class: | C12N 9/88 20130101; C12Y 402/02002 20130101 |
International Class: | C12N 9/88 20060101 C12N009/88 |
Date | Code | Application Number |
---|---|---|
Nov 2, 2015 | TW | 104135987 |
Sequence CWU 1
1
111858DNAPaenibacillus campinasensis BL-11 1gatggtgacg gtacaacagc
ctctatcgag caaatcttga gaaaccagag acctgacggt 60ggatggagaa aggactattc
tgtcacctct ggagaatggg caaagtctac cattgacaac 120aaagccacat
acaccgagat cagaagattg gccgcagaat acaccaagac aagagattct
180agatattctg acgccgccgt cagaggaatc cacttcttgt tgaacatgca
atacgccaac 240ggtggttggc ctcagatcta tcagggaact ggttaccaca
gacatatcac ctacaacgac 300aacgctatga tcaacgtcat gttgttgttg
gatgatgtcg ccaacagaag aggagatttt 360gccttcgtta acacatcttt
ggccgatcag tctagagccg ccgttactag aggtgttgat 420tgcattttga
gaacacaggt tgtcgccggt ggaagattga ctgcttgggg tcagcaacat
480gactctgtct ctttggcacc agcaggagct agatcttacg aagtcccttc
tttgaccgcc 540tctgagtcta ctggtatcgt cagattcttg aagaccagac
cacagacctc tcagattaga 600gcatctatcc aggcagctga agcctggttc
cagaccgtca aaatcactgg aatcagagtc 660gttaagacca acgacgatgt
tatcgtcgtc gaagatccat ctgtcacaac ccctatctgg 720gccagatttt
acgaaatcgg aaccaacaga ccaatcttcg tcggtagaga cggaatcgtc
780aagtacagat tgtctgagat cgagcaggaa agaagaaccg gttactcttg
gtacggtaat 840tggccagcat ctttgttt 8582286PRTPaenibacillus
campinasensis BL-11 2Asp Gly Asp Gly Thr Thr Ala Ser Ile Glu Gln
Ile Leu Arg Asn Gln 1 5 10 15 Arg Pro Asp Gly Gly Trp Arg Lys Asp
Tyr Ser Val Thr Ser Gly Glu 20 25 30Trp Ala Lys Ser Thr Ile Asp Asn
Lys Ala Thr Tyr Thr Glu Ile Arg 35 40 45Arg Leu Ala Ala Glu Tyr Thr
Lys Thr Arg Asp Ser Arg Tyr Ser Asp 50 55 60Ala Ala Val Arg Gly Ile
His Phe Leu Leu Asn Met Gln Tyr Ala Asn 65 70 75 80Gly Gly Trp Pro
Gln Ile Tyr Gln Gly Thr Gly Tyr His Arg His Ile 85 90 95Thr Tyr Asn
Asp Asn Ala Met Ile Asn Val Met Leu Leu Leu Asp Asp 100 105 110Val
Ala Asn Arg Arg Gly Asp Phe Ala Phe Val Asn Thr Ser Leu Ala 115 120
125Asp Gln Ser Arg Ala Ala Val Thr Arg Gly Val Asp Cys Ile Leu Arg
130 135 140Thr Gln Val Val Ala Gly Gly Arg Leu Thr Ala Trp Gly Gln
Gln His 145 150 155 160Asp Ser Val Ser Leu Ala Pro Ala Gly Ala Arg
Ser Tyr Glu Val Pro 165 170 175Ser Leu Thr Ala Ser Glu Ser Thr Gly
Ile Val Arg Phe Leu Lys Thr 180 185 190Arg Pro Gln Thr Ser Gln Ile
Arg Ala Ser Ile Gln Ala Ala Glu Ala 195 200 205Trp Phe Gln Thr Val
Lys Ile Thr Gly Ile Arg Val Val Lys Thr Asn 210 215 220Asp Asp Val
Ile Val Val Glu Asp Pro Ser Val Thr Thr Pro Ile Trp 225 230 235
240Ala Arg Phe Tyr Glu Ile Gly Thr Asn Arg Pro Ile Phe Val Gly Arg
245 250 255Asp Gly Ile Val Lys Tyr Arg Leu Ser Glu Ile Glu Gln Glu
Arg Arg 260 265 270Thr Gly Tyr Ser Trp Tyr Gly Asn Trp Pro Ala Ser
Leu Phe 275 280 2853858DNAArtificial SequenceSynthetically
generated DNA encoding a modified enzyme 3gatggtgacg gtacaacagc
ctctatcgag caaatcttga gaaaccagag acctgacggt 60ggatggagaa aggactattc
tgtcacctct ggagaatggg caaagtctac cattgacaac 120aaagccacat
acaccgagat cagaagattg gccgcagaat acaccaagac aagagattct
180agatattctg acgccgccgt cagaggaatc cacttcttgt tgaacatgca
atacgccaac 240ggtggttggc ctcagatcta tcagggaact ggttaccaca
gacatatcac ctacaacgac 300aacgctatga tcaacgtcat gttgttgttg
gatgatgtcg ccaacagaag aggagatttt 360gccttcgtta acacatcttt
ggccgatcag tctagagccg ccgttactag aggtgttgat 420tgcattttga
gaacacaggt tgtcgccggt ggaagattga ctgcttgggg tcagcaacat
480gactctgtct ctttggcacc agcaggagct agatcttacg aagtcccttc
tttgaccgcc 540ttcgagtcta ctggtatcgt cagattcttg aagaccagac
cacagacctc tcagattaga 600gcatctatcc aggcagctga agcctggttc
cagaccgtca aaatcactgg aatcagagtc 660gttaagacca acgacgatgt
tatcgtcgtc gaagatccat ctgtcacaac ccctatctgg 720gccagatttt
acgaaatcgg aaccaacaga ccaatcttcg tcggtagaga cggaatcgtc
780aagtacagat tgtctgagat cgagcaggaa agaagaaccg gttactcttg
gtacggtaat 840tggccagcat ctttgttt 8584286PRTArtificial
SequenceSequence synthetically translated from SEQ ID NO 3 4Asp Gly
Asp Gly Thr Thr Ala Ser Ile Glu Gln Ile Leu Arg Asn Gln 1 5 10 15
Arg Pro Asp Gly Gly Trp Arg Lys Asp Tyr Ser Val Thr Ser Gly Glu 20
25 30Trp Ala Lys Ser Thr Ile Asp Asn Lys Ala Thr Tyr Thr Glu Ile
Arg 35 40 45Arg Leu Ala Ala Glu Tyr Thr Lys Thr Arg Asp Ser Arg Tyr
Ser Asp 50 55 60Ala Ala Val Arg Gly Ile His Phe Leu Leu Asn Met Gln
Tyr Ala Asn 65 70 75 80Gly Gly Trp Pro Gln Ile Tyr Gln Gly Thr Gly
Tyr His Arg His Ile 85 90 95Thr Tyr Asn Asp Asn Ala Met Ile Asn Val
Met Leu Leu Leu Asp Asp 100 105 110Val Ala Asn Arg Arg Gly Asp Phe
Ala Phe Val Asn Thr Ser Leu Ala 115 120 125Asp Gln Ser Arg Ala Ala
Val Thr Arg Gly Val Asp Cys Ile Leu Arg 130 135 140Thr Gln Val Val
Ala Gly Gly Arg Leu Thr Ala Trp Gly Gln Gln His 145 150 155 160Asp
Ser Val Ser Leu Ala Pro Ala Gly Ala Arg Ser Tyr Glu Val Pro 165 170
175Ser Leu Thr Ala Phe Glu Ser Thr Gly Ile Val Arg Phe Leu Lys Thr
180 185 190Arg Pro Gln Thr Ser Gln Ile Arg Ala Ser Ile Gln Ala Ala
Glu Ala 195 200 205Trp Phe Gln Thr Val Lys Ile Thr Gly Ile Arg Val
Val Lys Thr Asn 210 215 220Asp Asp Val Ile Val Val Glu Asp Pro Ser
Val Thr Thr Pro Ile Trp 225 230 235 240Ala Arg Phe Tyr Glu Ile Gly
Thr Asn Arg Pro Ile Phe Val Gly Arg 245 250 255Asp Gly Ile Val Lys
Tyr Arg Leu Ser Glu Ile Glu Gln Glu Arg Arg 260 265 270Thr Gly Tyr
Ser Trp Tyr Gly Asn Trp Pro Ala Ser Leu Phe 275 280
2855858DNAArtificial SequenceSynthetically generated DNA encoding a
modified enzyme 5gatggtgacg gtacaacagc ctctatcgag caaatcttga
gaaaccagag acctgacggt 60ggatggagaa aggactattc tgtcacctct ggagaatggg
caaagtctac cattgacaac 120aaagccacat acaccgagat cagaagattg
gccgcagaat acaccaagac aagagattct 180agatattctg acgccgccgt
cagaggaatc cacttcttgt tgaacatgca atacgccaac 240ggtggttggc
ctcagatcta tcagggaact ggttaccaca gacatatcac ctacaacgac
300aacgctatga tcaacgtcat gttgttgttg gatgatgtcg ccaacagaag
aggagatttt 360gccttcgtta acacatcttt ggccgatcag tctagagccg
ccgttactag aggtgttgat 420tgcattttga gaacacaggt tgtcgccggt
ggaagattga ctgcttgggg tcagcaacat 480gactctgtct ctttggcacc
agcaggagct agatcttacg aagtcccttc tttgaccgcc 540atggagtcta
ctggtatcgt cagattcttg aagaccagac cacagacctc tcagattaga
600gcatctatcc aggcagctga agcctggttc cagaccgtca aaatcactgg
aatcagagtc 660gttaagacca acgacgatgt tatcgtcgtc gaagatccat
ctgtcacaac ccctatctgg 720gccagatttt acgaaatcgg aaccaacaga
ccaatcttcg tcggtagaga cggaatcgtc 780aagtacagat tgtctgagat
cgagcaggaa agaagaaccg gttactcttg gtacggtaat 840tggccagcat ctttgttt
8586286PRTArtificial SequenceSequence synthetically translated from
SEQ ID NO 5 6Asp Gly Asp Gly Thr Thr Ala Ser Ile Glu Gln Ile Leu
Arg Asn Gln 1 5 10 15 Arg Pro Asp Gly Gly Trp Arg Lys Asp Tyr Ser
Val Thr Ser Gly Glu 20 25 30Trp Ala Lys Ser Thr Ile Asp Asn Lys Ala
Thr Tyr Thr Glu Ile Arg 35 40 45Arg Leu Ala Ala Glu Tyr Thr Lys Thr
Arg Asp Ser Arg Tyr Ser Asp 50 55 60Ala Ala Val Arg Gly Ile His Phe
Leu Leu Asn Met Gln Tyr Ala Asn 65 70 75 80Gly Gly Trp Pro Gln Ile
Tyr Gln Gly Thr Gly Tyr His Arg His Ile 85 90 95Thr Tyr Asn Asp Asn
Ala Met Ile Asn Val Met Leu Leu Leu Asp Asp 100 105 110Val Ala Asn
Arg Arg Gly Asp Phe Ala Phe Val Asn Thr Ser Leu Ala 115 120 125Asp
Gln Ser Arg Ala Ala Val Thr Arg Gly Val Asp Cys Ile Leu Arg 130 135
140Thr Gln Val Val Ala Gly Gly Arg Leu Thr Ala Trp Gly Gln Gln His
145 150 155 160Asp Ser Val Ser Leu Ala Pro Ala Gly Ala Arg Ser Tyr
Glu Val Pro 165 170 175Ser Leu Thr Ala Met Glu Ser Thr Gly Ile Val
Arg Phe Leu Lys Thr 180 185 190Arg Pro Gln Thr Ser Gln Ile Arg Ala
Ser Ile Gln Ala Ala Glu Ala 195 200 205Trp Phe Gln Thr Val Lys Ile
Thr Gly Ile Arg Val Val Lys Thr Asn 210 215 220Asp Asp Val Ile Val
Val Glu Asp Pro Ser Val Thr Thr Pro Ile Trp 225 230 235 240Ala Arg
Phe Tyr Glu Ile Gly Thr Asn Arg Pro Ile Phe Val Gly Arg 245 250
255Asp Gly Ile Val Lys Tyr Arg Leu Ser Glu Ile Glu Gln Glu Arg Arg
260 265 270Thr Gly Tyr Ser Trp Tyr Gly Asn Trp Pro Ala Ser Leu Phe
275 280 2857858DNAArtificial SequenceSynthetically generated DNA
encoding a modified enzyme 7gatggtgacg gtacaacagc ctctatcgag
caaatcttga gaaaccagag acctgacggt 60ggatggagaa aggactattc tgtcacctct
ggagaatggg caaagtctac cattgacaac 120aaagccacat acaccgagat
cagaagattg gccgcagaat acaccaagac aagagattct 180agatattctg
acgccgccgt cagaggaatc cacttcttgt tgaacatgca atacgccaac
240ggtggttggc ctcagatcta tcagggaact ggttaccaca gacatatcac
ctacaacgac 300aacgctatga tcaacgtcat gttgttgttg gatgatgtcg
ccaacagaag aggagatttt 360gccttcgtta acacatcttt ggccgatcag
tctagagccg ccgttactag aggtgttgat 420tgcattttga gaacacaggt
tgtcgccggt ggaagattga ctgcttgggg tcagcaacat 480gactctgtct
ctttggcacc agcaggagct agatcttacg aagtcccttc tttgaccgcc
540ttggagtcta ctggtatcgt cagattcttg aagaccagac cacagacctc
tcagattaga 600gcatctatcc aggcagctga agcctggttc cagaccgtca
aaatcactgg aatcagagtc 660gttaagacca acgacgatgt tatcgtcgtc
gaagatccat ctgtcacaac ccctatctgg 720gccagatttt acgaaatcgg
aaccaacaga ccaatcttcg tcggtagaga cggaatcgtc 780aagtacagat
tgtctgagat cgagcaggaa agaagaaccg gttactcttg gtacggtaat
840tggccagcat ctttgttt 8588286PRTArtificial SequenceSequence
synthetically translated from SEQ ID NO 7 8Asp Gly Asp Gly Thr Thr
Ala Ser Ile Glu Gln Ile Leu Arg Asn Gln 1 5 10 15 Arg Pro Asp Gly
Gly Trp Arg Lys Asp Tyr Ser Val Thr Ser Gly Glu 20 25 30Trp Ala Lys
Ser Thr Ile Asp Asn Lys Ala Thr Tyr Thr Glu Ile Arg 35 40 45Arg Leu
Ala Ala Glu Tyr Thr Lys Thr Arg Asp Ser Arg Tyr Ser Asp 50 55 60Ala
Ala Val Arg Gly Ile His Phe Leu Leu Asn Met Gln Tyr Ala Asn 65 70
75 80Gly Gly Trp Pro Gln Ile Tyr Gln Gly Thr Gly Tyr His Arg His
Ile 85 90 95Thr Tyr Asn Asp Asn Ala Met Ile Asn Val Met Leu Leu Leu
Asp Asp 100 105 110Val Ala Asn Arg Arg Gly Asp Phe Ala Phe Val Asn
Thr Ser Leu Ala 115 120 125Asp Gln Ser Arg Ala Ala Val Thr Arg Gly
Val Asp Cys Ile Leu Arg 130 135 140Thr Gln Val Val Ala Gly Gly Arg
Leu Thr Ala Trp Gly Gln Gln His 145 150 155 160Asp Ser Val Ser Leu
Ala Pro Ala Gly Ala Arg Ser Tyr Glu Val Pro 165 170 175Ser Leu Thr
Ala Leu Glu Ser Thr Gly Ile Val Arg Phe Leu Lys Thr 180 185 190Arg
Pro Gln Thr Ser Gln Ile Arg Ala Ser Ile Gln Ala Ala Glu Ala 195 200
205Trp Phe Gln Thr Val Lys Ile Thr Gly Ile Arg Val Val Lys Thr Asn
210 215 220Asp Asp Val Ile Val Val Glu Asp Pro Ser Val Thr Thr Pro
Ile Trp 225 230 235 240Ala Arg Phe Tyr Glu Ile Gly Thr Asn Arg Pro
Ile Phe Val Gly Arg 245 250 255Asp Gly Ile Val Lys Tyr Arg Leu Ser
Glu Ile Glu Gln Glu Arg Arg 260 265 270Thr Gly Tyr Ser Trp Tyr Gly
Asn Trp Pro Ala Ser Leu Phe 275 280 285933DNAArtificial
SequenceSynthetically generated primer 9ccttctttga ccgccttcga
gtctactggt atc 331033DNAArtificial SequenceSynthetically generated
primer 10ccttctttga ccgccatgga gtctactggt atc 331133DNAArtificial
SequenceSynthetically generated primer 11ccttctttga ccgccttgga
gtctactggt atc 33
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