U.S. patent application number 15/293487 was filed with the patent office on 2017-02-02 for method including a converting process for separating nanoparticles with a controlled number of active groups.
The applicant listed for this patent is CHUNG YUAN CHRISTIAN UNIVERSITY. Invention is credited to Walter Hong-Shong Chang, Jimmy Kuan-Jung Li, Wolfgang Parak, Teresa Pellegrino, Ralph Alexander Sperling.
Application Number | 20170030920 15/293487 |
Document ID | / |
Family ID | 38139835 |
Filed Date | 2017-02-02 |
United States Patent
Application |
20170030920 |
Kind Code |
A1 |
Chang; Walter Hong-Shong ;
et al. |
February 2, 2017 |
Method including a Converting Process for Separating Nanoparticles
with a Controlled Number of Active groups
Abstract
The present invention discloses a method for separating
nanoparticles with a controlled number of active groups is
disclosed. First, a plurality of nanoparticles are provided,
wherein the surface of the nanoparticle comprises a plurality of
first active groups. Next, a plurality of functional ligands are
provided, wherein the functional ligand comprises at least one
second active group and at least one third active group. Then, a
binding process is performed to bind the nanoparticle with the
functional ligand, wherein the first active group connects with the
second active group. After the binding process, a converting
process and a separation process are performed to isolate a
plurality of nanoparticles with a controlled number of the fifth
active groups. The controlled number is integers from 0 to 10.
Inventors: |
Chang; Walter Hong-Shong;
(Tao-Yuan City, TW) ; Li; Jimmy Kuan-Jung;
(Tao-Yuan City, TW) ; Sperling; Ralph Alexander;
(Eltville, DE) ; Pellegrino; Teresa; (Lecce,
IT) ; Parak; Wolfgang; (Munich, DE) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
CHUNG YUAN CHRISTIAN UNIVERSITY |
Tao-Yuan |
|
TW |
|
|
Family ID: |
38139835 |
Appl. No.: |
15/293487 |
Filed: |
October 14, 2016 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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11302240 |
Dec 14, 2005 |
9494593 |
|
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15293487 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
G01N 33/587
20130101 |
International
Class: |
G01N 33/58 20060101
G01N033/58 |
Claims
1. A method for separating nanoparticles with a controlled number
of active groups, comprising: providing a plurality of
nanoparticles, wherein the surface of said nanoparticle comprises a
plurality of first active groups; providing a plurality of
functional ligands, wherein the molecular weight of said functional
ligand is larger than or equal to 1000 g/mol, and said functional
ligand comprises at least one second active group and at least one
third active group; performing a binding process to bind said
nanoparticle with said functional ligand, wherein said first active
group connects with said second active group; performing a
converting process to convert said third active group of said
nanoparticle into a fifth active group; and performing a separation
process to isolate a plurality of nanoparticles with a controlled
number of said fifth active groups.
2. The method as claimed in claim 1, wherein the diameter of said
nanoparticle is smaller than 50 nm.
3. The method as claimed in claim 1, wherein the nanoparticle
comprises one of the following group: quantum dot, metallic
nanoparticle, and metal oxide nanoparticle.
4. The method as claimed in claim 1, wherein said nanoparticle has
said plurality of first active groups.
5. The method as claimed in claim 1, wherein said nanoparticle is
bound with said plurality of first active groups.
6. The method as claimed in claim 1, further comprising a
modification process to modify the surface of said nanoparticle
with said first active group by an amphiphilic oligomer or
polymer.
7. The method as claimed in claim 1, wherein said functional ligand
further comprises a spacer bound with said second active group and
said third active group.
8. The method as claimed in claim 7, wherein said spacer comprises
oligomer or polymer.
9. The method as claimed in claim 8, wherein said oligomer or
polymer comprises any one or any combination of the group
consisting of: polyol [e.g., polyethylene glycol, polypropylene
glycol, polytetramethylene glycol, poly(oxyethylene) glycol,
poly(oxypropylene) poly(oxyethylene) triol, polycarbonate glyco],
acrylate based oligomer or polymer, vinyl based oligomer or
polymer.
10. The method as claimed in claim 1, wherein said functional
ligand further comprises a spacer having said third active group
therein, and bound with said second active group.
11. The method as claimed in claim 10, wherein said spacer
comprises biological molecule except nucleic acids.
12. The method as claimed in claim 1, wherein said functional
ligand further comprises a spacer having said second active group
and said third active group therein.
13. The method as claimed in claim 12, wherein said spacer
comprises biological molecule except nucleic acids.
14. The method as claimed in claim 1, wherein said first active
groups, said second active group, and said third active group are
independently selected from the group consisting of: a) chemical
functional group; b) biological molecule; c) protecting group.
15. The method as claimed in claim 1, wherein the connecting type
between said first active group and said second active group is
chemical bonding or physical bonding.
16. The method as claimed in claim 1, wherein said converting
process comprises: providing a plurality of converters, wherein
said converter comprises a fourth active group and at least one
fifth active group; reacting said third active group of said
nanoparticle with said fourth active group, so as to form a
plurality of nanoparticles with said fifth active groups.
17. The method as claimed in claim 1, wherein said fourth active
group and said fifth active group are independently selected from
the group consisting of: a) chemical functional group; b)
biological molecule; c) protecting group.
18. The method as claimed in claim 1, wherein said separation
process comprises one of the following group: size exclusion
chromatography (SEC) and gel electrophoresis.
19. The method as claimed in claim 1, wherein said separation
process is gel electrophoresis, and the molecular weight of said
functional ligand is larger than 3000 g/mol.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This is a divisional of U.S. application Ser. No.
11/302,240, filed Dec. 14, 2005 by the same inventors, and claims
priority there from. This divisional application contains rewritten
claims to the restricted-out subject matter of original claims.
BACKGROUND OF THE INVENTION
[0002] 1. Field of the Invention
[0003] The present invention is generally related to a method for
separating nanoparticles, and more particularly to a method for
separating nanoparticles with a controlled number of active
groups.
[0004] 2. Description of the Prior Art
[0005] Nanoparticle labels with a discrete and controlled number of
attached ligands (or even more general: functional groups) would be
very desirable. Dependent on material, size, and shape,
nanoparticles can have different functionalities, such as
fluorescence, phosphorescence, optical absorption, or magnetic
moment, and can thus be detected with different techniques. Ligand
molecules attached to the surface of such nanoparticles will
specifically bind to their corresponding receptors. Such
constructs, as for instance gold or semiconductor nanoparticles
decorated with oligonucleotides, streptavidin or antibodies, have
been successfully used in life sciences to trace the position of
single proteins within the membrane of living cells, and to
visualize the structure of artificially created nanostructures.
[0006] One key issue for some of the above-mentioned applications
is the ability to control the number of ligand molecules bound to
each nanoparticle. By exactly controlling the number of binding
sites per nanoparticle unwanted crosslinking effects between the
labels or between the structures to be labeled, which eventually
can lead to agglomeration, can be avoided. For the controlled
assembly of nanoparticle groupings such defined building blocks are
a prerequisite. Except few cases, so far it has not been possible
to directly synthesize such nanoparticles. Therefore, new method
for separating nanoparticles with a controlled number of active
groups is still needed corresponding to both economic effect and
utilization in industry.
SUMMARY OF THE INVENTION
[0007] In accordance with the present invention, new method for
separating nanoparticles with a controlled number of active groups
is provided.
[0008] One object of the present invention is to employ functional
ligands, wherein the functional ligand can have at least one
binding group and at least one active group. If the functional
ligand(s) bound to a nanoparticle by the binding group change its
overall effective size sufficiently enough, fractions of
nanoparticles with a different number of functional ligands can be
then separated. Therefore, nanoparticles with a controlled number
of the active groups can be sorted out.
[0009] Another object of the present invention is to render
hydrophobic nanoparticles hydrophilic before the binding process,
such as: coating by amphiphilic polymers (alternating or
block-copolymers) or lipids, so as to synthesize nanoparticles of
different materials (such as fluorescent or magnetic ones) that
have an identical surface. For this reason also the concept of the
attachment of functional ligands per nanoparticle is not restricted
to one type of nanoparticles but should be applicable for
nanoparticles of most materials. Therefore, this present invention
does have the economic advantages for industrial applications.
[0010] Accordingly, the present invention discloses a method for
separating nanoparticles with a controlled number of active groups.
First, a plurality of nanoparticles are provided, wherein the
surface of the nanoparticle comprises a plurality of first active
groups. Next, a plurality of functional ligands are provided,
wherein the functional ligand comprises at least one second active
group and at least one third active group. Then, a binding process
is performed to bind the nanoparticle with the functional ligand,
wherein the first active group connects with the second active
group. After the binding process, a separation process is performed
to isolate a plurality of nanoparticles with a controlled number of
the third active groups. The controlled number is integers from 0
to 10.
BRIEF DESCRIPTION OF THE DRAWINGS
[0011] FIG. 1 is a flow chart of a method for separating
nanoparticles with a controlled number of active groups in
accordance with the first embodiment of the present invention;
[0012] FIG. 2A shows the nanoparticle has the first active groups X
thereon, and FIG. 2B shows the nanoparticle is bound with the first
active groups X;
[0013] FIG. 3A shows the functional ligand comprises a spacer bound
with the second active group Y and the third active group Z, FIG.
3B shows the functional ligand comprises a spacer having the third
active group Z therein, and bound with the second active group Y,
and FIG. 3C shows the functional ligand comprises a spacer having
the second active group Y and the third active group Z therein;
[0014] FIGS. 4(a) to 4(h) illustrates gel electrophoresis of PEG/Au
conjugates. Different amino-modified PEG molecules were added to
polymer-coated Au nanoparticles (core diameter ca. 4 nm), where
FIG. 4(a) is NH.sub.2-PEG, molecular weight M=750 g/mol, FIG. 4 (b)
is NH.sub.2-PEG , M=2000 g/mol, FIG. 4(c) is NH.sub.2-PEG, M=5000
g/mol, FIG. 4(d) is NH.sub.2-PEG, M=10000 g/mol, FIG. 4(e) is
NH.sub.2-PEG-NH.sub.2, M=897 g/mol, FIG. 4(f) is
NH.sub.2-PEG-NH.sub.2, M=3000 g/mol, FIG. 4(g) is
NH.sub.2-PEG-NH.sub.2, M=6000 g/mol, and FIG. 4(h) is
NH.sub.2-PEG-NH.sub.2, M=10000 g/mol;
[0015] FIG. 5 shows addition of NHS-PEG-biotin to Au-nanoparticles
with no NH.sub.2-group or with exactly one NH.sub.2-group per
nanoparticle;
[0016] FIG. 6(a) is gel electrophoresis of PEG/Au and FIG. 6(b) is
PEG/CdSe/ZnS conjugates; and
[0017] FIG. 7 is a flow chart of a method for separating
nanoparticles with a controlled number of active groups in
accordance with the second embodiment of the present invention.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0018] What probed into the invention is a method for separating
nanoparticles with a controlled number of active groups. Detailed
descriptions of the production, structure and elements will be
provided in the following in order to make the invention thoroughly
understood. Obviously, the application of the invention is not
confined to specific details familiar to those who are skilled in
the art. On the other hand, the common elements and procedures that
are known to everyone are not described in details to avoid
unnecessary limits of the invention. Some preferred embodiments of
the present invention will now be described in greater detail in
the following. However, it should be recognized that the present
invention can be practiced in a wide range of other embodiments
besides those explicitly described, that is, this invention can
also be applied extensively to other embodiments, and the scope of
the present invention is expressly not limited except as specified
in the accompanying claims.
[0019] Definitions
[0020] The term "conjugate" herein refers to nanoparticle bound
with functional ligand(s).
[0021] The term "nanoparticle" can be made of organic, inorganic or
metal material, and more preferred refers to metal and metal oxide
nanoparticles or semiconductor nanocrystals. "Semiconductor
nanocrystals" herein is used synonymously with the term colloidal
"quantum dot" as commonly understood and herein refers to
nanocrystals that are composed of a semiconducting material, such
as: IIA-VIA semiconductors, IIA-VA semiconductors, IVA-IVA
semiconductors, and IVA-VIA semiconductors, and are made in such a
way as to crystallize in exceedingly small sizes, e.g. from 2-20 nm
in diameter. The semiconductor nanocrystals used herein are
colloidal, which refers to the fact that the semiconductor
nanocrystals are dispersed within a continuous medium in a manner
that prevents them from being filtered easily or settled rapidly.
The semiconductor nanocrystals used herein luminesce or upon
excitation by a light source. The semiconductor nanocrystals used
herein preferably are modified to be hydrophilic and may be further
modified to contain chemical functional groups, crosslinkers,
biological molecules and combinations thereof.
[0022] The term, "biological molecule" herein refers to molecules
including, by way of example only, such classes of substances as
monoclonal and polyclonal antibodies, nucleic acids (both monomeric
and oligomeric), proteins, enzymes, lipids, polysaccharides,
sugars, peptides, polypeptides, drugs, and bioligands.
[0023] The term "connect" herein refers to describe the
relationship between the first active group and the second active
group, or between the third active group and the fourth active
group. For example, the first active group is connected to said
second active group through chemical or physical interaction (e.g.
covalent bond, coordination bond, van der Waals force, hydrogen
bond, etc.).
[0024] Referring to FIG. 1, in a first embodiment of the present
invention, a method for separating nanoparticles with a controlled
number of active groups is disclosed. First, a plurality of
nanoparticles 110 are provided, wherein the surface of the
nanoparticle 110 comprises a plurality of first active groups. The
preferred diameter of the nanoparticle 110 is smaller than 50 nm,
and the nanoparticle 110 comprises one of the following group:
quantum dot (e.g., CdS, CdSe, CdTe, ZnSe, ZnS, PbS, PbSe, and their
alloys), metallic nanoparticle (e.g., gold, silver, copper,
titanium, nickel, platinum, palladium, cobalt, and their alloys),
metal oxide nanoparticle, doped metal oxide particles, metalloid
and metalloid oxide nanoparticles, the lanthanide series metal
nanoparticles, and combinations thereof. Furthermore, there are two
preferred constructs of the nanoparticles 110: a) as shown in FIG.
2A, the nanoparticle 110 has the first active groups X thereon; b)
as shown in FIG. 2B, the nanoparticle 110 is bound with the first
active groups X. For example, the surface of the nanoparticle 110
is modified to be bound with the first active group by an
amphiphilic oligomer or polymer. Next, a plurality of functional
ligands 120 are provided, wherein the functional ligand 120
comprises at least one second active group and at least one third
active group. Then, a binding process 135 is performed to bind the
nanoparticle 110 with the functional ligand 120, wherein the first
active group connects with the second active group through chemical
bonding or physical bonding. Finally, a separation process 140 is
performed to isolate a plurality of nanoparticles with a controlled
number of the third active groups 145, wherein the controlled
number is integers from 0 to 10.
[0025] Furthermore, the preferred molecular weight of the
functional ligand is larger than or equal to 1000 g/mol, and the
separation process comprises size exclusion chromatography (SEC)
and gel electrophoresis, wherein SEC comprises Gel Chromatography.
However, for nanoparticles with different size, different
separation methods, different operational parameters (e.g.
temperature, gel species), or different functional ligands, the
molecular weight limit of the functional ligand might be varied. In
a preferred example of this embodiment, 3000 g/mol is a better
lower limit for the separation process.
[0026] In this embodiment, three preferred constructs of the
functional ligand 120 are illustrated: a) as shown in FIG. 3A, the
functional ligand 120 comprises a spacer bound with the second
active group Y and the third active group Z, wherein the spacer
comprises oligomer or polymer; b) as shown in FIG. 3B, the
functional ligand 120 comprises a spacer having the third active
group Z therein, and bound with the second active group Y, wherein
the spacer comprises biological molecule except nucleic acids; c)
as shown in FIG. 3C, the functional ligand 120 comprises a spacer
having the second active group Y and the third active group Z
therein, wherein the spacer comprises biological molecule except
nucleic acids. Furthermore, the mentioned oligomer or polymer of
the functional ligand in construct a) comprises any one or any
combination of the group consisting of: polyol [e.g., polyethylene
glycol, polypropylene glycol, polytetramethylene glycol,
poly(oxyethylene) glycol, poly(oxypropylene) poly(oxyethylene)
triol, polycarbonate glyco], acrylate based oligomer or polymer,
vinyl based oligomer or polymer.
[0027] Additionally, the mentioned first active groups, the second
active group, and the third active group are independently selected
from the group consisting of:
[0028] a) chemical functional group, such as: sulfonic group,
hydroxyl group, amino group, sulfhydryl group, carboxyl group,
epoxy group, isocyanate group, organic halide group, maleimidyl
group, alkoxy group, succinimidyl group, ortho-pyridylthiolic
group, ortho-pyridyldisulfidyl group, vinylsulfonic group, acrylate
group, alkyl ketone group, hydrazine group, hydrazide group,
thioester group, and aldehydyl group.
[0029] b) biological molecule as described in definitions
[0030] c) protecting group, such as: Fmoc group, Boc group
[0031] Moreover, it is noteworthy that "biological molecule" can be
used as one kind of active group or one kind of functional ligand.
The major difference is molecular weight, wherein the MW of
biological molecule as active groups is smaller than that of
biological molecule as functional ligand. For example, "small
biological molecules" comprises biotin, cystein, benzylguanine,
peptides, small aptamers (DNA oligomers, RNA oligomers, PNA
oligomers), etc. On the other hand, "big biological molecules"
comprises antibodies, aptamers, avidin, neutravidin, stepavidin,
etc.
[0032] In this embodiment, a converting process 150 can be
performed after the separation process 140, so as to convert the
third active group of the nanoparticle into a fifth active group.
One example of the converting process 150 comprises: (1) providing
a plurality of converters 130, wherein the converter 130 comprises
a fourth active group and at least one fifth active group, such as:
NHS-PEG-biotin; (2) connecting the third active group of the
nanoparticle 110 with the fourth active group, so as to form a
plurality of nanoparticles with a controlled number of the fifth
active groups 155. The fourth active group and the fifth active
group are independently selected from the group consisting of:
[0033] a) chemical functional group, such as: sulfonic group,
hydroxyl group, amino group, sulfhydryl group, carboxyl group,
epoxy group, isocyanate group, organic halide group, maleimidyl
group, alkoxy group, succinimidyl group, ortho-pyridylthiolic
group, ortho-pyridyldisulfidyl group, vinylsulfonic group, acrylate
group, alkyl ketone group, hydrazine group, hydrazide group,
thioester group, and aldehydyl group.
[0034] b) biological molecule as described in definitions
[0035] c) protecting group, such as: Fmoc group, Boc group
[0036] Another example of the converting process 150 comprises a
redox reaction to reduce or oxidize the third active group to the
fifth active group. Still another example of the converting process
150 comprises a deprotecting reaction. For a preferred case, the
third active group is Fmoc-protected or Boc-protected amino group,
and piperidine or TFA can be used as deprotecting agent
respectively.
[0037] In this embodiment, the functional ligand can further
comprise at least one cleaving site between said second active
group and said third active group. Therefore, after the completion
of the separation process, a cleaving process is performed to break
said cleaving site. Then, there are two kinds of results: a) the
cleaving site is broken to form a sixth active group; b) the
functional ligand originally comprises a seventh active group
between said second active group and said cleaving site, and the
seventh active group remains bound to the nanoparticle after the
cleavage. In this way, also the same exact number of a shorter
functional ligand bound to the nanoparticle can be obtained.
Additionally, some preferred examples are listed as following: when
the cleaving site is disulfide bond, the cleaving process uses a
reduction agent as cleaving agent, such as: dithiothreitol (DTT),
tris(2-carboxyethyl)phosphine hydrochloride (TCEP); when the
cleaving site is peptide or protein, the cleaving process uses
trypsin as cleaving agent; when the cleaving site is peptide or
protein or DNA or RNA or PNA, the cleaving process uses enzymes as
cleaving agent.
EXAMPLE 1
[0038] High quality inorganic nanoparticles of many materials with
excellent size distribution, which have been synthesized in organic
solvents, can be transferred to aqueous solution by embedding them
in an amphiphilic polymer shell. (see FIG. 1a) (reference 1: Wu, M.
X.; Liu, H.; Liu, J.; Haley, K. N.; Treadway, J. A.; Larson, J. P.;
Ge, N.; Peale, F.; Bruchez, M. P. Nature Biotechnology 2003, 21,
452)(reference 2: Pellegrino, T.; Manna, L.; Kudera, S.; Liedl, T.;
Koktysh, D.; Rogach, A. L.; Keller, S.; Radler, J.; Natile, G.;
Parak, W. J. Nanoletters 2004, 4, (4), 703-707) This process yields
nanoparticles with an excellent size distribution (i.e. the size
distribution does not get significantly worse due to the polymer
shell) that yield narrow bands in gel electrophoresis.
[0039] By covalently attaching mono- or bifunctional short
polyethylene glycol (PEG) functional ligands to this polymer shell,
the size of the nanoparticles increased with the number and the
molecular weight of the attached molecules. The binding can thus be
monitored by gel electrophoresis [FIG. 4(a) to (h)]. This approach
results in stable water-soluble nanoparticles of different
materials with identical surface chemistry and many functional
groups on their outside (e.g. --NH.sub.2 on the end of the PEG
which is pointing towards solution). The covalent linkage of
(biological) molecules to the surface of such nanoparticles has
been demonstrated by using crosslinker. PEG functional ligands on
the nanoparticle surface simplify the bioconjugation of
nanoparticles. Nanoparticles are typically stabilized in aqueous
solution by electrostatic repulsion. Since also many biological
molecules are charged, repulsive interactions between the molecule
and the nanoparticle (for likewise charged nanoparticles and
molecules) or electrostatic adsorption (for oppositely charged
nanoparticles and molecules) can occur. In order to suppress
unwanted repulsive charge interactions salt can be added to screen
the charge of the nanoparticles and molecules. However, this
eventually leads to an agglomeration of the nanoparticles, which do
not repel each other anymore with sufficient force. On the other
hand, nanoparticles modified with PEG on their surface repel each
other by steric interaction and the bioconjugation can be performed
at higher salt concentrations. In this way PEG reduces the risk of
nanoparticle agglomeration and enhanced the binding yield.
EXAMPLE 2
[0040] Amino-modified PEG bearing an amino group only on one end of
the PEG chain (hereinafter as NH.sub.2-PEG, regardless of the
unmodified end thereof') has been attached to the --COOH groups of
the surface of polymer-coated Au nanoparticles with standard
bioconjugation chemistry using 1-Ethyl-3-(3-Dimethylaminopropyl)
carbodiimide Hydrochloride (EDC). The more NH.sub.2-PEG functional
ligands are bound per Au nanoparticle the bigger the resulting
conjugate becomes. This can be easily observed using
gel-electrophoresis: Negatively charged polymer-coated Au
nanoparticles migrate towards the positive pole and they become the
more retarded the more NH.sub.2-PEG functional ligands has been
attached [see FIG. 4(a) and FIG. 4(b)]. If NH.sub.2-PEG functional
ligand with a molecular weight 5000 g/mol is used discrete bands
can be observed [see FIG. 4(c) and FIG. 4(d)]. In analogy to the
well known DNA/Au-nanoparticle conjugates we ascribe these bands to
Au-nanoparticles with no, exactly one, exactly two, etc.
NH.sub.2-PEG functional ligands attached per nanoparticle. To test
this assumption we performed the same control experiments as have
been used in the case of DNA/Au-nanoparticle conjugates (as
performed by the Alivisatos group) as will be described below. In
order to introduce functional groups as anchor points for further
attachment of biological molecules, PEG functional ligand with two
modified ends are used. PEG modified with amino groups at both ends
(hereinafter as NH.sub.2-PEG-NH.sub.2) is covalently attached to
the surface of polymer-coated Au nanoparticles using EDC as
described above. The appropriate choice of the concentrations
prevented inter-particle crosslinking. This is confirmed by
comparing the migration of Au-nanoparticles modified under the same
conditions with NH.sub.2-PEG and NH.sub.2-PEG-NH.sub.2 with gel
electrophoresis [see FIG. 4(e), FIG. 4(f), FIG. 4(g) and FIG.
4(h)]. Nanoparticles modified with NH.sub.2-PEG-NH.sub.2 exhibit
free amino groups on their surface so that molecules bearing a
N-hydroxysuccinimide ester (NHS) functionality can be directly
attached via the formation of a covalent bond. We demonstrated this
for the case of NHS-PEG-biotin resulting in biotin-modified
nanoparticles. In the following the key experiments are described
in more detail.
[0041] Length Dependence:
[0042] Gel electrophoresis of PEG/Au conjugates is shown in FIG.
4(a) to (h). Different amino-modified PEG functional ligands were
added to polymer-coated Au nanoparticles (core diameter ca. 4 nm):
FIG. 4 (a) is NH.sub.2-PEG, molecular weight M=750 g/mol, FIG. 4(b)
is NH.sub.2-PEG , M=2000 g/mol, FIG. 4(c) is NH.sub.2-PEG, M=5000
g/mol, FIG. 4(d) is NH.sub.2-PEG, M=10000 g/mol, FIG. 4(e) is
NH.sub.2-PEG-NH.sub.2, M=897 g/mol, FIG. 4(f) is
NH.sub.2-PEG-NH.sub.2, M=3000 g/mol, FIG. 4(g) is
NH.sub.2-PEG-NH.sub.2, M=6000 g/mol, and FIG. 4(h) is
NH.sub.2-PEG-NH.sub.2, M=10000 g/mol. The PEG functional ligands
(shown in curved lines) were attached via their NH.sub.2-group to
the COOH-groups of the polymer-shell of the Au nanoparticles (shown
in white core with striped shell) with EDC. The amount of attached
PEG functional ligands per Au nanoparticle was adjusted by changing
the concentration of EDC in the following range:
c(EDC)/c(Au-nanoparticle)=0, 31, 63, 125, 250, 500, 1000, 2000,
4000, 8000, 16000, 32000, 64000, 128000. After sufficient
incubation time the reaction mixtures were loaded on a 2% agarose
gel and run for 80-90 minutes. Due to their negative charge the
conjugates migrate towards the plus pole. The first band (on the
top of the gel) corresponds to the reaction mixture c(EDC)/c(Au)=0,
the second one to c(EDC)/c(Au-nanoparticle)=31, etc. This means
that the bands from the top to the bottom lane of each gel
correspond to an increased number of PEG functional ligands
attached per Au-nanoparticle. A dashed line marks the position on
the gel where the conjugates have been loaded. The first lane of
each gel corresponds to the reaction mixture to which no EDC has
been added and thus no PEG has been attached to the nanoparticles.
The more PEG is attached per nanoparticle the more the bands are
retarded. At one point the nanoparticle surface is saturated with
PEG and even the addition of more EDC does not result in an
increase in size. This can be seen in the bands on the bottom of
the gels, where the speed of migration does not decrease anymore
upon the addition of more EDC. Even at saturation with PEG there
are still enough free negatively charged --COOH groups available on
the polymer surface (e.g. FIG. 4(a)). For PEG with high molecular
weight discrete bands on the gel can be seen [see FIG. 4(c), FIG.
4(d), FIG. 4(g), and FIG. 4(h)]. We ascribe these bands to
Au-nanoparticles with no, exactly one, and exactly two PEG
functional ligands bound per nanoparticle. For PEG with low
molecular weight no discrete bands can be observed and the number
of bound PEG functional ligands per Au-nanoparticle cannot be
resolved. In the case of saturation (for short and long PEG) the
Au-nanoparticle surface is covered to the highest possible extent
with PEG. However, the number of PEG functional ligands bound per
nanoparticle can not be deduced from our data in this case.
[0043] For short NH.sub.2-PEG functional ligands (molecular weight
<5000 g/mol) the change in size due to the addition of one
single PEG is too small to be detectable with gel electrophoresis.
If more PEG is attached per nanoparticle the size of the conjugates
gets continuously bigger and the bands on the gel are more
retarded. At one point the nanoparticle surface is saturated with
PEG, and the retardation of the nanoparticles on the gels remains
constant [see FIG. 4(a)]. The higher the molecular weight of the
PEG is, the bigger the maximum retardation of the conjugates
becomes [see FIG. 4(a), FIG. 4(b), FIG. 4(c) and FIG. 4(d)]. At
molecular weights 5000 g/mol the change upon binding of one PEG
functional ligand to the nanoparticle surface yields a size change
big enough to be detected as discrete band on the gel [see FIG.
4(c) and FIG. 4(d)]. The higher the molecular weight of the PEG is,
the bigger the retardation on the gel upon the addition of one
single PEG becomes [see FIG. 4(c) and FIG. 4(d)]. Upon saturation
with long PEG the retardation of the conjugates even leads to a
change in the direction of migration [see FIG. 4(b), FIG. 4(c) and
FIG. 4(d)]. We speculate that this effect might be associated with
the complexation of positively charged ions with the neutral
PEG.
[0044] We have strong experimental evidence that, similar to DNA/Au
nanoparticle conjugates, the main effect for retardation of the
bands on the gel upon binding PEG to Au-nanoparticles is the change
in the overall size: Upon attachment of each NH.sub.2-PEG
functional ligand via bond formation between the NH.sub.2-group of
the PEG and a COOH-group on the nanoparticle surface one negative
charge on the nanoparticle surface (which originated from the
COOH-group) is lost. This effect does not depend on the length of
the PEG. However, since retardation on the gel was found to
increase with the length of the PEG functional ligands, this
retardation cannot be predominantly ascribed to the loss in
negative charge, because this effect does not depend on the length
of the PEG. For reasons of steric hindrance the maximum number of
PEG functional ligands that can be attached per nanoparticle will
decrease with the length of the PEG. Since in the case of
saturation of nanoparticles with short PEG (i.e. in the situation
when the maximum amount of PEG is attached per nanoparticle) the
conjugates migrate towards the plus pole, we can conclude that even
in the case of saturation PEG functional ligands have been only
been attached to a fraction of the --COOH groups on the
nanoparticle surface.
[0045] Introduction of Discrete Functional Groups:
[0046] NH.sub.2-PEG-NH.sub.2 of different molecular weight was
attached with EDC to the polymer surface of Au-nanoparticles as
described above for NH.sub.2-PEG. Gel electrophoresis experiments
demonstrated that the conjugates for Au-nanoparticles conjugated to
NH.sub.2-PEG-NH.sub.2 and NH.sub.2-PEG yield bands with comparable
retardation on the gel [see FIG. 4(e), FIG. 4(f), FIG. 4(g) and
FIG. 4(h)]. This means that inter-particle crosslinking, which only
would be possible in the case of NH.sub.2-PEG-NH.sub.2 if one PEG
binds with both NH.sub.2 groups to two different nanoparticles, can
be neglected. In case of inter-particle crosslinking the bands for
Au-nanoparticles conjugated to NH.sub.2-PEG-NH.sub.2 should be more
retarded than the one for Au-nanoparticles conjugated to
NH.sub.2-PEG. As for the NH.sub.2-PEG also for the
NH.sub.2-PEG-NH.sub.2 with a molecular weight 5000 g/mol discrete
bands could be observed with gel electrophoresis [see FIG. 4(g) and
FIG. 4(h)]. Since the bands can be ascribed to Au-nanoparticles
with no, exactly one, exactly two, etc. PEG functional ligands per
Au-nanoparticle these conjugates are in fact conjugates with a
precisely controlled number of reactive groups on their surface.
Whereas the polymer-surface of the Au-nanoparticles comprises only
accessible --COOH groups, each attached NH.sub.2-PEG-NH.sub.2 bears
one free --NH.sub.2 group at its end that is pointing away from the
nanoparticle. In this way conjugates with exactly one, two, etc.
--NH.sub.2 groups per nanoparticle are obtained.
[0047] Stability of the Conjugates:
[0048] As mentioned above, the retardation of the conjugates that
we ascribe to Au-nanoparticles with one and two bound PEG
functional ligands increases with the length of the PEG. The
formation of nonspecifically formed Au-nanoparticle clusters on the
other hand should not depend on the length of the PEG. This fact
strongly indicates that the discrete retarded bands cannot be
ascribed to nonspecifically formed dimers and trimers of
Au-nanoparticles. As further control we extracted the nanoparticles
within the discrete bands from the gel and run the purified and
re-concentrated sample again on a second gel (FIG. 5). Most of the
extracted conjugates were found to migrate with the same speed as
the original ones. Only a very weak band on the gel was found at
the position of Au nanoparticles without PEG. This indicates that
the conjugates are stable upon extraction from the gel,
purification, and re-concentration, and that only for a minor
fraction of the conjugates the PEG is removed from the
nanoparticles.
[0049] Reactivity of the Discrete Functional Groups:
[0050] Referring to FIG. 5, polymer-coated Au nanoparticles were
incubated with a 1:20 mixture of NH.sub.2-PEG-NH.sub.2 (molecular
weight M=10000 g/mol) and NH.sub.2-PEG (molecular weight M=750
g/mol) and the PEG was attached to the nanoparticles by adding EDC.
Conjugates were run for 1 hour on a 2% agarose gel. In lane 1 the
band of the pure Au nanoparticles and in lane 3 the two bands
resulting from the PEG-Au conjugates are shown. We ascribe the
faster band in lane 3 to Au nanoparticles to whose surface only
some of the short NH.sub.2-PEG have been attached. Due to these
attached PEG the conjugates migrate slightly slower than the pure
Au nanoparticles. The slower migrating band in lane 3 is ascribed
to Au nanoparticles to which some short NH.sub.2-PEG and exactly
one long NH.sub.2-PEG-NH.sub.2 have been attached. The attachment
of one single long NH.sub.2-PEG-NH.sub.2 is sufficient enough for a
significant retardation (cfg. FIG. 4(h). We have extracted the two
bands from lane 3 from the gel and run them again together with the
original sample. The fast migrating band of the extracted sample is
shown in lane 6, and the slow migrating band of the extracted
sample is shown in lane 4. The band in lane 4 migrates with the
same speed as the slow band in lane 3 and the band in lane 6
migrates with the same speed as the fast band in lane 3. This
demonstrates that conjugates can be stably extracted from the gel
without breaking the bond between the PEG functional ligands and
the Au-nanoparticles. We believe that the attached short
NH.sub.2-PEG functional ligands facilitate this stability.
Conjugates without some short NH.sub.2-PEG tended to aggregate
during extraction from the gel and the following purification and
concentration of the sample. We ascribe the more retarded band in
lane 3 to Au nanoparticles with exactly one attached
NH.sub.2-PEG-NH.sub.2 functional ligand (in addition to several
short NH.sub.2-PEG functional ligands). Therefore, each of these
conjugates should possess exactly one reactive --NH.sub.2 site (at
the end of the PEG pointing towards solution). We tested this
hypothesis by adding NHS-PEG-biotin (molecular weight M=5000
g/mol). The NHS group is reactive towards free NH.sub.2 groups.
Because of its height molecular weight the binding of
NHS-PEG-biotin should be visible as further retardation on the gel.
As control NHS-PEG-biotin was added to pure Au nanoparticles (see
lane 2) and to Au nanoparticles with only short NH.sub.2-PEG
functional ligands attached (which have been extracted from the
fast migrating band in lane 3, see lane 7). In both cases no shift
can be seen and therefore we can rule out non-specific attachment
of the NHS-PEG-biotin to the Au nanoparticles. However, when
NHS-PEG-biotin was added to the conjugates bearing exactly one
reactive NH.sub.2-group (which have been extracted from the slow
migrating band in lane 3) an additional band with enhanced
retardation can be observed (see lane 5). The yield of this
reaction is not 100%, since also a band at the position of the
original conjugates remains. However, the existence of the more
retarded band proves that the added NHS-PEG-biotin has been
specifically attached to the original conjugates. This band
corresponds to Au-nanoparticles with exactly one biotin-group per
nanoparticle.
[0051] As described above we were able to synthesize conjugates of
Au-nanoparticles with exactly one, two, etc. --NH.sub.2 groups per
nanoparticle. Using standard bioconjugation chemistry it should be
possible to convert the --NH.sub.2 groups to other functional
groups or to attach biomolecules. We have demonstrated this
possibility for the case of biotin by using NHS-modified biotin as
biomolecule. Every biotin bearing an NHS group should be reactive
towards the discrete --NH.sub.2 groups of the conjugates. We have
added NHS-PEG-biotin (5000 g/mol) to Au-nanoparticles with no or
exactly one --NH.sub.2 group per nanoparticle. As shown in FIG. 5,
addition of NHS-PEG-biotin to Au-nanoparticles with no
NH.sub.2-group basically did not result in any shift on the gel and
thus it can be derived that nonspecific adsorption of the PEG to
the nanoparticles does not play a mayor role (FIG. 5, lanes 2 and
7). In the case of Au-nanoparticles with exactly one NH.sub.2-group
per nanoparticle the addition of NHS-PEG-biotin results in the
formation of a second, more retarded band on the gel (FIG. 5, lane
5). This indicates that part of the Au-nanoparticles have reacted
with the PEG, which in turn increased the size of the conjugates
and thus reduced the mobility on the gel. This second band now
corresponds to Au-nanoparticles with exactly one biotin-group per
nanoparticle. However, besides the second retarded band, a band
remains that has the same mobility as the original nanoparticles
with one NH.sub.2-PEG per nanoparticle. This means that in these
experiment the yield in the conversion of the --NH.sub.2 group to a
-biotin group is not optimum.
[0052] Universality of the Concept:
[0053] Gel electrophoresis of PEG/Au and PEG/CdSe/ZnS conjugates is
shown in FIG. 6(a) and FIG. 6(b), respectively. Amino-modified PEG
functional ligands (NH.sub.2-PEG, M=5000 g/mol) were added to
polymer-coated Au nanoparticles (diameter of the Au core ca. 4 nm)
(FIG. 6(a)) and CdSe/ZnS nanoparticles (diameter of the CdSe/ZnS
core/shell ca. 7 nm) (FIG. 6(b)) and the number of attached PEG
functional ligands per nanoparticle was adjusted by using different
concentrations of EDC: c(EDC)/c(Au-nanoparticle) and
c(EDC)/c(CdSe/ZnS-nanoparticle) =0, 31, 63, 125, 250, 500, 1000,
2000, 4000, 8000, 16000, 32000, 64000, 128000. These conjugates
were run on a 2% agarose gel for 80-90 minutes and the lanes on the
gel correspond to the different reaction mixtures, whereby the top
lane corresponds to c(EDC)=0 and the bottom lane to the maximum EDC
concentration. FIG. 6(a) is identical to FIG. 4(c). By comparing
FIG. 6(a) and FIG. 6(b), it can be seen that the position of the
bands on the gel does not depend on the nature of the inorganic
nanoparticle material that is embedded in the polymer shell. In the
case of Au nanoparticles discrete bands corresponding to conjugates
in which exactly one, two, and three PEG functional ligands are
attached per nanoparticle can be clearly resolved. In the case of
the fluorescent CdSe/ZnS nanoparticles even bands of higher order
(four and five PEG functional ligands per nanoparticle) can be
resolved. This is due to the fact that the band of the pure
CdSe/ZnS nanoparticles (i.e. without attached PEG functional
ligands) is narrower than the band of the pure Au nanoparticles
(top lane with c(EDC)=0 in both gels). However, for the pure
CdSe/ZnS nanoparticles a weak second band can be observed. This
band has to be attributed either to some non-specifically formed
dimers of nanoparticles or to overloading the gel with
nanoparticles. However, this second band is slightly less retarded
than the band that we ascribe to CdSe/ZnS nanoparticles modified
with on PEG (compare lanes 1 and 2 of FIG. 6(b)) and therefore does
not interfere with the sorting process.
[0054] In previous work we have demonstrated that by embedding
nanoparticles in a polymer shell we can synthesize nanoparticles of
different materials (such as fluorescent or magnetic ones) that
have an identical surface. For this reason also the concept of the
attachment of individual functional ligands per nanoparticle is not
restricted to one type of nanoparticles but should be applicable
for nanoparticles of most materials. To demonstrate this
generalization we have also conjugated fluorescent
CdSe/ZnS-nanoparticles with individual NH.sub.2-PEG functional
ligands using the same protocols as have been applied for
Au-nanoparticles. As shown in FIG. 6, PEG/Au- and
CdSe/ZnS-nanoparticle conjugates show the same behavior upon gel
electrophoresis, which demonstrates that the conjugation reaction
does not depend on the properties of the inorganic nanoparticle
inside the polymer shell. In a next step we tried to demonstrate
that this concept should work also for different functional groups
X. By conjugating polymer-coated Au-nanoparticles with
NH.sub.2-PEG-X functional ligands with PEG of sufficient length
(molecular weight of the PEG 5000 g/mol) and running the conjugates
on a gel discrete bands corresponding to nanoparticles with exactly
no, one, two, etc. functional groups X per nanoparticle could be
obtained. We have demonstrated this for the case of X=NH.sub.2 and
without X (see FIG. 4(g), FIG. 4(h) and FIG. 4(c), FIG. 4(d),
respectively). Alternatively, by conjugating polymer-coated
Au-nanoparticles with NH.sub.2-PEG-NH.sub.2 functional ligands of
sufficient length (molecular weight of the PEG 5000 g/mol), running
the conjugates on a gel and extracting the discrete bands from the
gel conjugates corresponding to nanoparticles with exactly no, one,
two, etc. NH.sub.2-groups per nanoparticle were obtained. In a
following step the discrete NH.sub.2-groups could be converted to
functional groups by reacting them with NHS-X. We have demonstrated
this for the case of X=PEG-biotin (FIG. 5) reproduced the
converting also with X=PEG-maleimide and X=PEG-BOC.
[0055] Referring to FIG. 7, in a second embodiment of the present
invention, a method for separating nanoparticles with a controlled
number of active groups is disclosed. First, a plurality of
nanoparticles 710 are provided, wherein the surface of the
nanoparticle 710 comprises a plurality of first active groups. The
preferred diameter of the nanoparticle 710, the material of the
nanoparticle 710, and the preferred constructs of the nanoparticle
710 are described in the first embodiment. Next, a plurality of
functional ligands 720 are provided, wherein the functional ligand
720 comprises at least one second active group and at least one
third active group. The preferred constructs of the functional
ligand 720 are described in the first embodiment. Then, a binding
process 725 is performed to bind the nanoparticle 710 with the
functional ligand 720, wherein the first active group connects with
the second active group. After completion the binding process 725,
a converting process 730 is performed to convert the third active
group of the nanoparticle 710 into a fifth active group, so
nanoparticles with the fifth active groups 735 are then formed.
Finally, a separation process 740 is performed to isolate a
plurality of nanoparticles with a controlled number of the fifth
active groups 745, wherein the controlled number is integers from 0
to 10.
[0056] In this embodiment, the first active groups, the second
active group, and the third active group are independently selected
from the group consisting of:
[0057] a) chemical functional group, such as: sulfonic group,
hydroxyl group, amino group, sulfhydryl group, carboxyl group,
epoxy group, isocyanate group, organic halide group, maleimidyl
group, alkoxy group, succinimidyl group, ortho-pyridylthiolic
group, ortho-pyridyldisulfidyl group, vinylsulfonic group, acrylate
group, alkyl ketone group, hydrazine group, hydrazide group,
thioester group, and aldehydyl group.
[0058] b) biological molecule as described in definitions
[0059] c) protecting group, such as: Fmoc group, Boc group
[0060] In this embodiment, one example of the converting process
730 comprises: (1) providing a plurality of converters, wherein the
converter comprises a fourth active group and at least one fifth
active group; (2) connecting the third active group of the
nanoparticle 710 with the fourth active group, so as to form a
plurality of nanoparticles with the fifth active groups.
Furthermore, the fourth active group and the fifth active group are
independently selected from the group consisting of:
[0061] a) chemical functional group, such as: sulfonic group,
hydroxyl group, amino group, sulfhydryl group, carboxyl group,
epoxy group, isocyanate group, organic halide group, maleimidyl
group, alkoxy group, succinimidyl group, ortho-pyridylthiolic
group, ortho-pyridyldisulfidyl group, vinylsulfonic group, acrylate
group, alkyl ketone group, hydrazine group, hydrazide group,
thioester group, and aldehydyl group.
[0062] b) biological molecule as described in definitions
[0063] c) protecting group, such as: Fmoc group, Boc group
[0064] Another example of the converting process 730 comprises a
redox reaction to reduce or oxidize the third active group to the
fifth active group. Still another example of the converting process
730 comprises a deprotecting reaction. For a preferred case, the
third active group is Fmoc-protected or Boc-protected amino group,
and piperidine or TFA can be used as deprotecting agent
respectively.
[0065] In this embodiment, the preferred molecular weight of the
functional ligand is larger than or equal to 1000 g/mol, and the
separation process comprises size exclusion chromatography (SEC)
and gel electrophoresis, wherein SEC comprises Gel Chromatography.
However, for nanoparticles with different size, different
separation methods, different operational parameters (e.g.
temperature, gel species), or different functional ligands, the
molecular weight limit of the functional ligand might be varied. In
a preferred example of this embodiment, 3000 g/mol is a better
lower limit for the separation process.
[0066] In the above preferred embodiments, the present invention
employs functional ligands, wherein the functional ligand can have
at least one binding group and at least one active group. If the
functional ligand(s) bound to a nanoparticle by the binding group
change its overall effective size sufficiently enough, fractions of
nanoparticles with a different number of functional ligands can be
then separated. Therefore, nanoparticles with a controlled number
of the active groups can be sorted out. The concept of the
attachment of a defined number of functional ligands per
nanoparticle is not restricted to one type of nanoparticles but
should be applicable for nanoparticles of most materials, or shell
types with functional groups, respectively. Therefore, this present
invention does have the economic advantages for industrial
applications. Therefore, this present invention does have the
economic advantages for industrial applications.
[0067] To sum up, the present invention discloses a method for
separating nanoparticles with a controlled number of active groups.
First, a plurality of nanoparticles are provided, wherein the
surface of the nanoparticle comprises a plurality of first active
groups. Next, a plurality of functional ligands are provided,
wherein the functional ligand comprises at least one second active
group and at least one third active group. Then, a binding process
is performed to bind the nanoparticle with the functional ligand,
wherein the first active group connects with the second active
group. After the binding process, a separation process is performed
to isolate a plurality of nanoparticles with a controlled number of
the third active groups. The controlled number is integers from 0
to 10.
[0068] Obviously many modifications and variations are possible in
light of the methods described above. It is therefore to be
understood that within the scope of the appended claims the present
invention can be practiced otherwise than as specifically described
herein. Although specific embodiments have been illustrated and
described herein, it is obvious to those skilled in the art that
many modifications of the present invention may be made without
departing from what is intended to be limited solely by the
appended claims.
* * * * *