U.S. patent application number 15/006048 was filed with the patent office on 2016-12-08 for polypeptides having cellulase activity. The applicant listed for this patent is California Institute of Technology. Invention is credited to Frances H. Arnold, Pete Heinzelman.
Application Number | 20160355799 15/006048 |
Document ID | / |
Family ID | 42826496 |
Filed Date | 2016-12-08 |
United States Patent Application | 20160355799 |
Kind Code | A1 |
Arnold; Frances H. ; et al. | December 8, 2016 |
The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
Inventors: | Arnold; Frances H.; (La Canada, CA) ; Heinzelman; Pete; (Norman, OK) | ||||||||||
Applicant: |
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Family ID: | 42826496 | ||||||||||
Appl. No.: | 15/006048 | ||||||||||
Filed: | January 25, 2016 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
12755328 | Apr 6, 2010 | 9249401 | ||
15006048 | ||||
61166993 | Apr 6, 2009 | |||
61177882 | May 13, 2009 | |||
Current U.S. Class: | 1/1 |
Current CPC Class: | C12N 9/2437 20130101; C12Y 302/01091 20130101; C12P 19/14 20130101; C07K 2319/00 20130101; C12Y 302/01004 20130101 |
International Class: | C12N 9/42 20060101 C12N009/42 |
Sequence CWU 1
1
10711083DNAHumicola insolensCDS(1)..(1083) 1ggt aac ccc ttt gaa ggt
gtt cag ctg tgg gct aat aac tat tat aga 48Gly Asn Pro Phe Glu Gly
Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 tct gag gta cat
aca ctg gcc att ccg caa att aca gac ccc gcg ttg 96Ser Glu Val His
Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 cgt gcc
gca gct agt gct gtg gct gag gtg cca agt ttt caa tgg ctg 144Arg Ala
Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu 35 40 45
gac aga aat gta aca gtg gat act ttg ttg gta cag act ttg tca gaa
192Asp Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu Ser Glu
50 55 60 atc cgt gag gcc aat caa gca ggt gct aat ccc caa tat gca
gcg caa 240Ile Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Gln Tyr Ala
Ala Gln 65 70 75 80 atc gtg gtc tat gat ctg ccc gat aga gac tgt gca
gct gcc gcc tcg 288Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala
Ala Ala Ala Ser 85 90 95 aat ggt gaa tgg gca ata gcg aac aac ggt
gta aac aat tac aaa gct 336Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly
Val Asn Asn Tyr Lys Ala 100 105 110 tac att aat aga att aga gag ata
ttg ata agt ttt tcg gac gtt aga 384Tyr Ile Asn Arg Ile Arg Glu Ile
Leu Ile Ser Phe Ser Asp Val Arg 115 120 125 acg ata tta gtc att gag
cca gat agt cta gct aat atg gtc aca aat 432Thr Ile Leu Val Ile Glu
Pro Asp Ser Leu Ala Asn Met Val Thr Asn 130 135 140 atg aat gtc ccg
aag tgt tcc ggt gca gcc agc act tat agg gaa tta 480Met Asn Val Pro
Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu 145 150 155 160 acc
ata tat gca ctg aag caa ttg gat ctg cct cat gtc gct atg tac 528Thr
Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr 165 170
175 atg gat gcc ggc cac gct gga tgg tta ggc tgg ccg gca aac att cag
576Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln
180 185 190 cca gcc gca gaa ttg ttt gcc aaa att tac gaa gat gct gga
aag cct 624Pro Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp Ala Gly
Lys Pro 195 200 205 aga gca gtg aga ggt ctt gca act aat gtt gct aat
tac aat gca tgg 672Arg Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn
Tyr Asn Ala Trp 210 215 220 tca gtt tca tcc cct cca cca tac aca agt
cca aat cca aac tac gat 720Ser Val Ser Ser Pro Pro Pro Tyr Thr Ser
Pro Asn Pro Asn Tyr Asp 225 230 235 240 gaa aag cat tat atc gaa gca
ttc aga ccc tta tta gaa gcc cgt ggt 768Glu Lys His Tyr Ile Glu Ala
Phe Arg Pro Leu Leu Glu Ala Arg Gly 245 250 255 ttc cca gcc caa ttt
ata gtg gat cag gga aga tca ggt aag caa cca 816Phe Pro Ala Gln Phe
Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro 260 265 270 act ggc caa
aag gag tgg ggg cat tgg tgt aat gct att ggc aca gga 864Thr Gly Gln
Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly 275 280 285 ttt
ggt atg aga cct act gct aat acc ggt cac cag tat gtg gat gct 912Phe
Gly Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala 290 295
300 ttt gtt tgg gtt aaa ccg ggc ggt gaa tgc gac ggg acc agc gat act
960Phe Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr
305 310 315 320 acg gcg gcc aga tat gat tat cat tgt ggt ctg gaa gat
gca tta aaa 1008Thr Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp
Ala Leu Lys 325 330 335 cca gct cct gaa gcc ggc cag tgg ttc aac gaa
tac ttc att caa ttg 1056Pro Ala Pro Glu Ala Gly Gln Trp Phe Asn Glu
Tyr Phe Ile Gln Leu 340 345 350 ctt agg aac gct aac ccg ccc ttt taa
1083Leu Arg Asn Ala Asn Pro Pro Phe 355 360 2360PRTHumicola
insolens 2Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr
Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr
Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val
Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr
Leu Leu Val Gln Thr Leu Ser Glu 50 55 60 Ile Arg Glu Ala Asn Gln
Ala Gly Ala Asn Pro Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val Tyr
Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn Gly
Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala 100 105 110
Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg 115
120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr
Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr
Arg Glu Leu 145 150 155 160 Thr Ile Tyr Ala Leu Lys Gln Leu Asp Leu
Pro His Val Ala Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly Trp
Leu Gly Trp Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu Phe
Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val Arg
Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Val
Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235
240 Glu Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly
245 250 255 Phe Pro Ala Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys
Gln Pro 260 265 270 Thr Gly Gln Lys Glu Trp Gly His Trp Cys Asn Ala
Ile Gly Thr Gly 275 280 285 Phe Gly Met Arg Pro Thr Ala Asn Thr Gly
His Gln Tyr Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly Gly
Glu Cys Asp Gly Thr Ser Asp Thr 305 310 315 320 Thr Ala Ala Arg Tyr
Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys 325 330 335 Pro Ala Pro
Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu 340 345 350 Leu
Arg Asn Ala Asn Pro Pro Phe 355 360 31077DNAHypocrea
jecorinaCDS(1)..(1077) 3ggt aat cca ttc gtt ggg gtg aca ccc tgg gcg
aac gcc tat tat gct 48Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala
Asn Ala Tyr Tyr Ala 1 5 10 15 tct gag gtt tca tcc cta gct att ccc
tct tta aca ggt gca atg gct 96Ser Glu Val Ser Ser Leu Ala Ile Pro
Ser Leu Thr Gly Ala Met Ala 20 25 30 aca gcc gcc gct gcc gtt gca
aag gtc cct tcc ttc atg tgg ctg gat 144Thr Ala Ala Ala Ala Val Ala
Lys Val Pro Ser Phe Met Trp Leu Asp 35 40 45 act ttg gac aaa acc
ccc tta atg gaa caa acg ttg gct gat ata cgt 192Thr Leu Asp Lys Thr
Pro Leu Met Glu Gln Thr Leu Ala Asp Ile Arg 50 55 60 act gcg aat
aaa aac ggc ggc aat tat gct gga caa ttt gtg gtt tat 240Thr Ala Asn
Lys Asn Gly Gly Asn Tyr Ala Gly Gln Phe Val Val Tyr 65 70 75 80 gac
ctg ccg gat aga gat tgt gct gca cta gcg agc aac ggg gag tac 288Asp
Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu Tyr 85 90
95 agc att gcg gat ggc ggt gtc gca aag tac aaa aac tat ata gat act
336Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp Thr
100 105 110 atc agg caa ata gtt gtc gaa tac agt gat att cgt acg ctg
ctt gta 384Ile Arg Gln Ile Val Val Glu Tyr Ser Asp Ile Arg Thr Leu
Leu Val 115 120 125 atc gaa ccc gat tcc tta gcg aac ttg gta aca aat
cta ggt act ccg 432Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr Asn
Leu Gly Thr Pro 130 135 140 aag tgt gcg aac gcg cag agt gct tat ctt
gag tgc atc aat tat gca 480Lys Cys Ala Asn Ala Gln Ser Ala Tyr Leu
Glu Cys Ile Asn Tyr Ala 145 150 155 160 gtc acc cag ttg aat ttg cca
aac gtt gca atg tat ctt gat gct ggt 528Val Thr Gln Leu Asn Leu Pro
Asn Val Ala Met Tyr Leu Asp Ala Gly 165 170 175 cat gcc ggg tgg ttg
ggt tgg cca gca aat cag gat ccc gct gcg cag 576His Ala Gly Trp Leu
Gly Trp Pro Ala Asn Gln Asp Pro Ala Ala Gln 180 185 190 ctg ttt gca
aat gtt tac aaa aat gcc tca agt cct aga gcg ctg agg 624Leu Phe Ala
Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu Arg 195 200 205 ggt
ctt gca aca aat gtt gct aat tac aac gga tgg aat att acc tca 672Gly
Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly Trp Asn Ile Thr Ser 210 215
220 ccc cca tca tac aca caa gga aat gct gtt tac aat gaa aaa ctt tat
720Pro Pro Ser Tyr Thr Gln Gly Asn Ala Val Tyr Asn Glu Lys Leu Tyr
225 230 235 240 att cat gcc att ggt cca ctg ctg gct aat cac gga tgg
agt aat gcc 768Ile His Ala Ile Gly Pro Leu Leu Ala Asn His Gly Trp
Ser Asn Ala 245 250 255 ttt ttc att aca gat caa ggg aga agt ggt aaa
caa cct act gga caa 816Phe Phe Ile Thr Asp Gln Gly Arg Ser Gly Lys
Gln Pro Thr Gly Gln 260 265 270 caa caa tgg ggt gac tgg tgt aat gtt
atc ggt act ggg ttt ggc atc 864Gln Gln Trp Gly Asp Trp Cys Asn Val
Ile Gly Thr Gly Phe Gly Ile 275 280 285 aga cca tca gcg aat acg ggt
gat tca ttg ttg gac tca ttt gtt tgg 912Arg Pro Ser Ala Asn Thr Gly
Asp Ser Leu Leu Asp Ser Phe Val Trp 290 295 300 gtt aaa ccc ggg ggt
gaa tgt gat gga acg agt gat tct tct gct cca 960Val Lys Pro Gly Gly
Glu Cys Asp Gly Thr Ser Asp Ser Ser Ala Pro 305 310 315 320 agg ttc
gat tct cat tgc gca tta cca gat gct ttg cag cca gca cct 1008Arg Phe
Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala Pro 325 330 335
caa gca gga gct tgg ttc caa gct tat ttt gta caa tta ctg act aac
1056Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu Thr Asn
340 345 350 gcc aat cct agt ttt cta taa 1077Ala Asn Pro Ser Phe Leu
355 4358PRTHypocrea jecorina 4Gly Asn Pro Phe Val Gly Val Thr Pro
Trp Ala Asn Ala Tyr Tyr Ala 1 5 10 15 Ser Glu Val Ser Ser Leu Ala
Ile Pro Ser Leu Thr Gly Ala Met Ala 20 25 30 Thr Ala Ala Ala Ala
Val Ala Lys Val Pro Ser Phe Met Trp Leu Asp 35 40 45 Thr Leu Asp
Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile Arg 50 55 60 Thr
Ala Asn Lys Asn Gly Gly Asn Tyr Ala Gly Gln Phe Val Val Tyr 65 70
75 80 Asp Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu
Tyr 85 90 95 Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr
Ile Asp Thr 100 105 110 Ile Arg Gln Ile Val Val Glu Tyr Ser Asp Ile
Arg Thr Leu Leu Val 115 120 125 Ile Glu Pro Asp Ser Leu Ala Asn Leu
Val Thr Asn Leu Gly Thr Pro 130 135 140 Lys Cys Ala Asn Ala Gln Ser
Ala Tyr Leu Glu Cys Ile Asn Tyr Ala 145 150 155 160 Val Thr Gln Leu
Asn Leu Pro Asn Val Ala Met Tyr Leu Asp Ala Gly 165 170 175 His Ala
Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro Ala Ala Gln 180 185 190
Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu Arg 195
200 205 Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly Trp Asn Ile Thr
Ser 210 215 220 Pro Pro Ser Tyr Thr Gln Gly Asn Ala Val Tyr Asn Glu
Lys Leu Tyr 225 230 235 240 Ile His Ala Ile Gly Pro Leu Leu Ala Asn
His Gly Trp Ser Asn Ala 245 250 255 Phe Phe Ile Thr Asp Gln Gly Arg
Ser Gly Lys Gln Pro Thr Gly Gln 260 265 270 Gln Gln Trp Gly Asp Trp
Cys Asn Val Ile Gly Thr Gly Phe Gly Ile 275 280 285 Arg Pro Ser Ala
Asn Thr Gly Asp Ser Leu Leu Asp Ser Phe Val Trp 290 295 300 Val Lys
Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Ser Ser Ala Pro 305 310 315
320 Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala Pro
325 330 335 Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu
Thr Asn 340 345 350 Ala Asn Pro Ser Phe Leu 355 51077DNAChaetomium
thermophiliumCDS(1)..(1077) 5ggt aac cct ttc agt ggt gtg cag tta
tgg gct aat act tac tat tct 48Gly Asn Pro Phe Ser Gly Val Gln Leu
Trp Ala Asn Thr Tyr Tyr Ser 1 5 10 15 tca gaa gtc cac acc tta gct
atc cca agc tta agt cca gaa tta gcg 96Ser Glu Val His Thr Leu Ala
Ile Pro Ser Leu Ser Pro Glu Leu Ala 20 25 30 gct aag gcg gcg aaa
gta gct gaa gtg cca tca ttc caa tgg tta gat 144Ala Lys Ala Ala Lys
Val Ala Glu Val Pro Ser Phe Gln Trp Leu Asp 35 40 45 aga aac gtg
act gtg gat act ctg ttt tct ggt aca ctt gct gag ata 192Arg Asn Val
Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu Ile 50 55 60 agg
gcg gct aac caa agg gga gct aat cca cca tat gct ggc atc ttt 240Arg
Ala Ala Asn Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe 65 70
75 80 gtg gtt tat gac ctt cct gat aga gat tgt gct gcc gct gca agc
aat 288Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser
Asn 85 90 95 ggt gaa tgg agt ata gct aac aac ggt gct aac aac tat
aag aga tat 336Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr
Lys Arg Tyr 100 105 110 atc gat aga att aga gaa ttg ttg att cag tac
tca gat atc agg aca 384Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr
Ser Asp Ile Arg Thr 115 120 125 att ttg gtt att gaa cca gac agt cta
gca aat atg gtt act aac atg 432Ile Leu Val Ile Glu Pro Asp Ser Leu
Ala Asn Met Val Thr Asn Met 130 135 140 aac gta caa aaa tgt tct aac
gca gca tct acg tat aaa gaa ctg act 480Asn Val Gln Lys Cys Ser Asn
Ala Ala Ser Thr Tyr Lys Glu Leu Thr 145 150 155 160 gtg tat gca ttg
aaa cag ttg aac ttg cca cac gta gcc atg tat atg 528Val Tyr Ala Leu
Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met 165 170 175 gat gca
ggt cac gcc ggc tgg tta ggc
tgg ccc gct aat ata cag cct 576Asp Ala Gly His Ala Gly Trp Leu Gly
Trp Pro Ala Asn Ile Gln Pro 180 185 190 gcc gca gaa tta ttc gcg caa
ata tac aga gac gct gga cgt ccg gct 624Ala Ala Glu Leu Phe Ala Gln
Ile Tyr Arg Asp Ala Gly Arg Pro Ala 195 200 205 gcg gtc agg ggt ctt
gcc act aac gtt gca aat tac aac gct tgg tca 672Ala Val Arg Gly Leu
Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 ata gcg agt
cct cca tcg tac aca agc cct aac cca aac tac gat gag 720Ile Ala Ser
Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240
aag cat tac ata gaa gca ttt gct cct ttg ctt cgt aac caa ggt ttt
768Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe
245 250 255 gat gca aag ttt atc gtc gat acc gga aga aac ggc aag cag
ccg aca 816Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln
Pro Thr 260 265 270 ggg cag cta gaa tgg ggg cac tgg tgc aat gtc aag
ggt acg ggt ttc 864Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys
Gly Thr Gly Phe 275 280 285 ggt gtt aga ccc acg gct aac act ggg cat
gag ttg gtt gat gca ttc 912Gly Val Arg Pro Thr Ala Asn Thr Gly His
Glu Leu Val Asp Ala Phe 290 295 300 gtt tgg gta aaa ccc gga gga gag
tca gac ggt act tct gat act agt 960Val Trp Val Lys Pro Gly Gly Glu
Ser Asp Gly Thr Ser Asp Thr Ser 305 310 315 320 gct gcc aga tac gat
tac cac tgt ggc ctt tct gat gct ttg aca cca 1008Ala Ala Arg Tyr Asp
Tyr His Cys Gly Leu Ser Asp Ala Leu Thr Pro 325 330 335 gcc cct gaa
gcc ggg caa tgg ttc cag gcc tac ttc gaa caa cta ttg 1056Ala Pro Glu
Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu 340 345 350 att
aac gca aac cca cca tag 1077Ile Asn Ala Asn Pro Pro 355
6358PRTChaetomium thermophilium 6Gly Asn Pro Phe Ser Gly Val Gln
Leu Trp Ala Asn Thr Tyr Tyr Ser 1 5 10 15 Ser Glu Val His Thr Leu
Ala Ile Pro Ser Leu Ser Pro Glu Leu Ala 20 25 30 Ala Lys Ala Ala
Lys Val Ala Glu Val Pro Ser Phe Gln Trp Leu Asp 35 40 45 Arg Asn
Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu Ile 50 55 60
Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe 65
70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala
Ser Asn 85 90 95 Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn
Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln
Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val Ile Glu Pro Asp Ser
Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn Val Gln Lys Cys Ser
Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr 145 150 155 160 Val Tyr Ala
Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met 165 170 175 Asp
Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro 180 185
190 Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro Ala
195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala
Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro
Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro
Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp
Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp
Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg
Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val
Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Thr Ser 305 310
315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Ser Asp Ala Leu Thr
Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu
Gln Leu Leu 340 345 350 Ile Asn Ala Asn Pro Pro 355
7267DNAArtificial SequenceCBD Linker 7gct agc tgc tca agc gtc tgg
ggc caa tgt ggt ggc cag aat tgg tcg 48Ala Ser Cys Ser Ser Val Trp
Gly Gln Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 ggt ccg act tgc tgt
gct tcc gga agc aca tgc gtc tac tcc aac gac 96Gly Pro Thr Cys Cys
Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 tat tac tcc
cag tgt ctt ccc ggc gct gca agc tca agc tcg tcc acg 144Tyr Tyr Ser
Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40 45 cgc
gcc gcg tcg acg act tct cga gta tcc ccc aca aca tcc cgg tcg 192Arg
Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 50 55
60 agc tcc gcg acg cct cca cct ggt tct act act acc aga gta cct cca
240Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro
65 70 75 80 gtc gga tcg gga acc gct acg tat tca 267Val Gly Ser Gly
Thr Ala Thr Tyr Ser 85 889PRTArtificial SequenceSynthetic Construct
8Ala Ser Cys Ser Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1
5 10 15 Gly Pro Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn
Asp 20 25 30 Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser
Ser Ser Thr 35 40 45 Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro
Thr Thr Ser Arg Ser 50 55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser
Thr Thr Thr Arg Val Pro Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr
Tyr Ser 85 921DNAArtificial SequenceOligonucleotide Primer CBH2L
9gctgaacgtg tcatcggtta c 211023DNAArtificial
SequenceOligonucleotide Primer RSQ3080 10gcaacacctg gcaattcctt acc
231124DNAArtificial SequenceOligonucleotide Primer CBH2LPCR
11gctgaacgtg tcatcgttac ttag 2412356PRTPhanerochaete chrysosporium
12Asn Asn Pro Trp Thr Gly Phe Gln Ile Phe Leu Ser Pro Tyr Tyr Ala 1
5 10 15 Asn Glu Val Ala Ala Ala Ala Lys Gln Ile Thr Asp Pro Thr Leu
Ser 20 25 30 Ser Lys Ala Ala Ser Val Ala Asn Ile Pro Thr Phe Thr
Trp Leu Asp 35 40 45 Ser Val Ala Lys Ile Pro Asp Leu Gly Thr Tyr
Leu Ala Ser Ala Ser 50 55 60 Ala Leu Gly Lys Ser Thr Gly Thr Lys
Gln Leu Val Gln Ile Val Ile 65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Lys Ala Ser Asn Gly Glu 85 90 95 Phe Ser Ile Ala Asn
Asn Gly Gln Ala Asn Tyr Glu Asn Tyr Ile Asp 100 105 110 Gln Ile Val
Ala Gln Ile Gln Gln Phe Pro Asp Val Arg Val Val Ala 115 120 125 Val
Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr Asn Leu Asn Val 130 135
140 Gln Lys Cys Ala Asn Ala Lys Thr Thr Tyr Leu Ala Cys Val Asn Tyr
145 150 155 160 Ala Leu Thr Asn Leu Ala Lys Val Gly Val Tyr Met Tyr
Met Asp Ala 165 170 175 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Leu Ser Pro Ala Ala 180 185 190 Gln Leu Phe Thr Gln Val Trp Gln Asn
Ala Gly Lys Ser Pro Phe Ile 195 200 205 Lys Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Leu Gln Ala Ala 210 215 220 Ser Pro Asp Pro Ile
Thr Gln Gly Asn Pro Asn Tyr Asp Glu Ile His 225 230 235 240 Tyr Ile
Asn Ala Leu Ala Pro Leu Leu Gln Gln Ala Gly Trp Asp Ala 245 250 255
Thr Phe Ile Val Asp Gln Gly Arg Ser Gly Val Gln Asn Ile Arg Gln 260
265 270 Gln Trp Gly Asp Trp Cys Asn Ile Lys Gly Ala Gly Phe Gly Thr
Arg 275 280 285 Pro Thr Thr Asn Thr Gly Ser Gln Phe Ile Asp Ser Ile
Val Trp Val 290 295 300 Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asn
Ser Ser Ser Pro Arg 305 310 315 320 Tyr Asp Ser Thr Cys Ser Leu Pro
Asp Ala Ala Gln Pro Ala Pro Glu 325 330 335 Ala Gly Thr Trp Phe Gln
Ala Tyr Phe Gln Thr Leu Val Ser Ala Ala 340 345 350 Asn Pro Pro Leu
355 13358PRTArtificial SequenceChimeric CBH II polypeptide 13Gly
Asn Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser 1 5 10
15 Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu Ala
20 25 30 Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Met Trp
Leu Asp 35 40 45 Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile Arg 50 55 60 Thr Ala Asn Lys Asn Gly Ala Asn Pro Pro
Tyr Ala Gly Ile Phe Val 65 70 75 80 Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn Gly 85 90 95 Glu Trp Ser Ile Ala Asn
Asn Gly Ala Asn Asn Tyr Lys Arg Tyr Ile 100 105 110 Asp Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr Ile 115 120 125 Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn 130 135 140
Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr Val 145
150 155 160 Tyr Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr
Met Asp 165 170 175 Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro Ala 180 185 190 Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp
Ala Gly Arg Pro Ala Ala 195 200 205 Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser Val 210 215 220 Ser Ser Pro Pro Pro Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys 225 230 235 240 His Tyr Ile
Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe Pro 245 250 255 Ala
Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr Gly 260 265
270 Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly Phe Gly
275 280 285 Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala
Phe Val 290 295 300 Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser
Asp Thr Ser Ala 305 310 315 320 Ala Arg Tyr Asp Tyr His Cys Gly Leu
Ser Asp Ala Leu Thr Pro Ala 325 330 335 Pro Glu Ala Gly Gln Trp Phe
Gln Ala Tyr Phe Glu Gln Leu Leu Ile 340 345 350 Asn Ala Asn Pro Pro
Phe 355 14360PRTArtificial SequenceChimeric CBH II polypeptide
14Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala
Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe
Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr Leu Phe Ser
Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn Gln Arg Gly Ala
Asn Pro Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val Tyr Asp Leu Pro
Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn Gly Glu Trp Ala
Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn
Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg 115 120 125 Thr
Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn 130 135
140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu
145 150 155 160 Thr Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val
Ala Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp
Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu Phe Ala Lys Ile
Tyr Glu Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val Arg Gly Leu Ala
Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Ile Ala Ser Pro
Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235 240 Glu Lys
His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly 245 250 255
Phe Asp Ala Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro 260
265 270 Thr Gly Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr
Gly 275 280 285 Phe Gly Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr
Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu Cys Asp
Gly Thr Ser Asp Thr 305 310 315 320 Ser Ala Ala Arg Tyr Asp Tyr His
Cys Gly Leu Ser Asp Ala Leu Thr 325 330 335 Pro Ala Pro Glu Ala Gly
Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu 340 345 350 Leu Ile Asn Ala
Asn Pro Pro Phe 355 360 15361PRTArtificial SequenceChimeric CBH II
Polypeptide 15Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Leu Val Gln Thr Leu Ser Glu 50 55 60 Ile Arg Glu Ala Asn
Gln Ala Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr
Tyr Lys Glu Leu 145 150 155 160 Thr Val Tyr Ala Leu Lys Gln Leu Asn
Leu Pro His Val Ala Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu
Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro 195
200 205 Ala Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala
Trp 210 215 220 Ser Val Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro
Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr Ile Glu Ala Phe Arg Pro
Leu Leu Glu Ala Arg Gly 245 250 255 Phe Pro Asn Ala Phe Phe Ile Thr
Asp Gln Gly Arg Ser Gly Lys Gln 260 265 270 Pro Thr Gly Gln Gln Gln
Trp Gly Asp Trp Cys Asn Val Ile Gly Thr 275 280 285 Gly Phe Gly Ile
Arg Pro Ser Ala Asn Thr Gly Asp Ser Leu Leu Asp 290 295 300 Ser Phe
Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp 305 310 315
320 Thr Thr Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu
325 330 335 Lys Pro Ala Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe
Ile Gln 340 345 350 Leu Leu Arg Asn Ala Asn Pro Pro Phe 355 360
16359PRTArtificial SequenceChimeric CBH II Polypeptide 16Gly Asn
Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala Tyr Tyr Ala 1 5 10 15
Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu Thr Gly Ala Met Ala 20
25 30 Thr Ala Ala Ala Ala Val Ala Lys Val Pro Ser Phe Gln Trp Leu
Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu
Ser Glu Ile 50 55 60 Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Gln
Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn
Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Arg Glu Leu Thr 145 150
155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr
Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp
Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala
Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr 260 265 270
Gly Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly Phe 275
280 285 Gly Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser
Asp Thr Thr 305 310 315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu
Glu Asp Ala Leu Lys Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe
Asn Glu Tyr Phe Ile Gln Leu Leu 340 345 350 Arg Asn Ala Asn Pro Pro
Phe 355 17357PRTArtificial SequenceChimeric CBH II Polypeptide
17Gly Asn Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu
Ala 20 25 30 Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Gln
Trp Leu Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln
Thr Leu Ser Glu Ile 50 55 60 Arg Glu Ala Asn Gln Ala Gly Gly Asn
Tyr Ala Gly Gln Phe Val Val 65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Leu Ala Ser Asn Gly Glu 85 90 95 Tyr Ser Ile Ala Asp
Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp 100 105 110 Arg Ile Arg
Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr Ile Leu 115 120 125 Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn Val 130 135
140 Pro Lys Cys Ser Gly Ala Ala Ser Ala Tyr Leu Glu Cys Ile Asn Tyr
145 150 155 160 Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr
Leu Asp Ala 165 170 175 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Gln Asp Pro Ala Ala 180 185 190 Gln Leu Phe Ala Asn Val Tyr Lys Asn
Ala Ser Ser Pro Arg Ala Leu 195 200 205 Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser Val Ser 210 215 220 Ser Pro Pro Pro Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys His 225 230 235 240 Tyr Ile
Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe Pro Ala 245 250 255
Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr Gly Gln 260
265 270 Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly Phe Gly
Met 275 280 285 Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala
Phe Val Trp 290 295 300 Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser
Asp Thr Thr Ala Ala 305 310 315 320 Arg Tyr Asp Tyr His Cys Gly Leu
Glu Asp Ala Leu Lys Pro Ala Pro 325 330 335 Glu Ala Gly Gln Trp Phe
Asn Glu Tyr Phe Ile Gln Leu Leu Arg Asn 340 345 350 Ala Asn Pro Pro
Phe 355 18357PRTArtificial SequenceChimeric CBH II Polypeptide
18Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala Tyr Tyr Ala 1
5 10 15 Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu Thr Gly Ala Met
Ala 20 25 30 Thr Ala Ala Ala Ala Val Ala Lys Val Pro Ser Phe Gln
Trp Leu Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly
Thr Leu Ala Glu Ile 50 55 60 Arg Ala Ala Asn Gln Arg Gly Gly Asn
Tyr Ala Gly Gln Phe Val Val 65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Leu Ala Ser Asn Gly Glu 85 90 95 Tyr Ser Ile Ala Asp
Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp 100 105 110 Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr Ile Leu 115 120 125 Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn Val 130 135
140 Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr Val Tyr
145 150 155 160 Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr
Met Asp Ala 165 170 175 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro Ala Ala 180 185 190 Glu Leu Phe Ala Gln Ile Tyr Arg Asp
Ala Gly Arg Pro Ala Ala Val 195 200 205 Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser Val Ser 210 215 220 Ser Pro Pro Pro Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys His 225 230 235 240 Tyr Ile
Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe Pro Ala 245 250 255
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr Gly Gln 260
265 270 Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe Gly
Val 275 280 285 Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe Val Trp 290 295 300 Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Thr Ser Ala Ala 305 310 315 320 Arg Tyr Asp Tyr His Cys Gly Leu
Ser Asp Ala Leu Thr Pro Ala Pro 325 330 335 Glu Ala Gly Gln Trp Phe
Gln Ala Tyr Phe Glu Gln Leu Leu Ile Asn 340 345 350 Ala Asn Pro Pro
Phe 355 19360PRTArtificial SequenceChimeric CBH II Polypeptide
19Gly Asn Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu
Ala 20 25 30 Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Gln
Trp Leu Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln
Thr Leu Ser Glu Ile 50 55 60 Arg Glu Ala Asn Gln Ala Gly Ala Asn
Pro Pro Tyr Ala Gly Ile Phe 65 70 75 80 Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ser Ile
Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg
Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr 115 120 125 Ile
Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135
140 Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu Thr
145 150 155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala
Met Tyr Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr
Glu Asp Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Val Ser Ser Pro Pro
Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His
Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe 245 250 255
Pro Ala Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr 260
265 270 Gly Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly
Phe 275 280 285 Gly Met Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val
Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly
Thr Ser Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys
Ala Leu Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala
Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn
Pro Ser Phe Leu 355 360 20356PRTArtificial SequenceChimeric CBH II
Polypeptide 20Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala
Tyr Tyr Ala 1 5 10 15 Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu
Thr Gly Ala Met Ala 20 25 30 Thr Ala Ala Ala Ala Val Ala Lys Val
Pro Ser Phe Met Trp Leu Asp 35 40 45 Thr Leu Asp Lys Thr Pro Leu
Met Glu Gln Thr Leu Ala Asp Ile Arg 50 55 60 Thr Ala Asn Lys Asn
Gly Gly Asn Tyr Ala Gly Gln Phe Val Val Tyr 65 70 75 80 Asp Leu Pro
Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu Tyr 85 90 95 Ser
Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp Arg 100 105
110 Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr Ile Leu Val
115 120 125 Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn
Val Pro 130 135 140 Lys Cys Ser Gly Ala Ala Ser Ala Tyr Leu Glu Cys
Ile Asn Tyr Ala 145 150 155 160 Val Thr Gln Leu Asn Leu Pro Asn Val
Ala Met Tyr Leu Asp Ala Gly 165 170 175 His Ala Gly Trp Leu Gly Trp
Pro Ala Asn Gln Asp Pro Ala Ala Gln 180 185 190 Leu Phe Ala Asn Val
Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu Arg 195 200 205 Gly Leu Ala
Thr Asn Val Ala Asn Tyr Asn Gly Trp Asn Ile Thr Ser 210 215 220 Pro
Pro Ser Tyr Thr Gln Gly Asn Ala Val Tyr Asn Glu Lys Leu Tyr 225 230
235 240 Ile His Ala Ile Gly Pro Leu Leu Ala Asn His Gly Trp Ser Ala
Lys 245 250 255 Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr
Gly Gln Leu 260 265 270 Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr
Gly Phe Gly Val Arg 275 280 285 Pro Thr Ala Asn Thr Gly His Glu Leu
Val Asp Ala Phe Val Trp Val 290 295 300 Lys Pro Gly Gly Glu Ser Asp
Gly Thr Ser Asp Thr Thr Ala Ala Arg 305 310 315 320 Tyr Asp Tyr His
Cys Gly Leu Glu Asp Ala Leu Lys Pro Ala Pro Glu 325 330 335 Ala Gly
Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu Leu Arg Asn Ala 340 345 350
Asn Pro Pro Phe 355 21358PRTArtificial SequenceChimeric CBH II
Polypeptide 21Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Gly Asn Tyr Ala Gly Gln Phe Val 65 70 75 80 Val Tyr Asp
Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly 85 90 95 Glu
Tyr Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile 100 105
110 Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr Ile
115 120 125 Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn
Met Asn 130 135 140 Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Arg
Glu Leu Thr Ile 145 150 155 160 Tyr Ala Leu Lys Gln Leu Asp Leu Pro
His Val Ala Met Tyr Met Asp 165 170 175 Ala Gly His Ala Gly Trp Leu
Gly Trp Pro Ala Asn Ile Gln Pro Ala 180 185 190 Ala Glu Leu Phe Ala
Lys Ile Tyr Glu Asp Ala Gly Lys Pro Arg Ala 195 200 205 Val Arg Gly
Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser Val 210 215 220 Ser
Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys 225 230
235 240 His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe
Pro 245 250 255 Ala Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln
Pro Thr Gly 260 265 270 Gln Lys Glu Trp Gly His Trp Cys Asn Ala Ile
Gly Thr Gly Phe Gly 275 280 285 Met Arg Pro Thr Ala Asn Thr Gly His
Gln Tyr Val Asp Ala Phe Val 290 295 300 Trp Val Lys Pro Gly Gly Glu
Cys Asp Gly Thr Ser Asp Thr Thr Ala 305 310
315 320 Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys Pro
Ala 325 330 335 Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln
Leu Leu Arg 340 345 350 Asn Ala Asn Pro Pro Phe 355
22357PRTArtificial SequenceChimeric CBH II Polypeptide 22Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala
Gly Gln Phe Val Val 65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp Cys Ala
Ala Leu Ala Ser Asn Gly Glu 85 90 95 Tyr Ser Ile Ala Asp Gly Gly
Val Ala Lys Tyr Lys Asn Tyr Ile Asp 100 105 110 Arg Ile Arg Glu Ile
Leu Ile Ser Phe Ser Asp Val Arg Thr Ile Leu 115 120 125 Val Ile Glu
Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn Val 130 135 140 Pro
Lys Cys Ser Gly Ala Ala Ser Thr Tyr Lys Glu Leu Thr Val Tyr 145 150
155 160 Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met Asp
Ala 165 170 175 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln
Pro Ala Ala 180 185 190 Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly
Arg Pro Ala Ala Val 195 200 205 Arg Gly Leu Ala Thr Asn Val Ala Asn
Tyr Asn Ala Trp Ser Val Ser 210 215 220 Ser Pro Pro Pro Tyr Thr Ser
Pro Asn Pro Asn Tyr Asp Glu Lys His 225 230 235 240 Tyr Ile Glu Ala
Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe Pro Ala 245 250 255 Gln Phe
Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr Gly Gln 260 265 270
Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr Gly Phe Gly Met 275
280 285 Arg Pro Thr Ala Asn Thr Gly His Gln Tyr Val Asp Ala Phe Val
Trp 290 295 300 Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr
Thr Ala Ala 305 310 315 320 Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp
Ala Leu Lys Pro Ala Pro 325 330 335 Glu Ala Gly Gln Trp Phe Asn Glu
Tyr Phe Ile Gln Leu Leu Arg Asn 340 345 350 Ala Asn Pro Pro Phe 355
23359PRTArtificial SequenceChimeric CBH II Polypeptide 23Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln
Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn
Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr 145 150
155 160 Val Tyr Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr
Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp
Ala Gly Arg Pro Ala 195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Thr Ser 305 310 315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu
Ser Asp Ala Leu Thr Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe
Gln Ala Tyr Phe Glu Gln Leu Leu 340 345 350 Ile Asn Ala Asn Pro Pro
Phe 355 24360PRTArtificial SequenceChimeric CBH II Polypeptide
24Gly Asn Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu
Ala 20 25 30 Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Gln
Trp Leu Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly
Thr Leu Ala Glu Ile 50 55 60 Arg Ala Ala Asn Gln Arg Gly Ala Asn
Pro Gln Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile
Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg
Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile
Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135
140 Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr
145 150 155 160 Val Tyr Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala
Met Tyr Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Gln Ile Tyr
Arg Asp Ala Gly Arg Pro Ala 195 200 205 Ala Val Arg Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Val Ser Ser Pro Pro
Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His
Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe 245 250 255
Pro Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260
265 270 Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly
Phe 275 280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val
Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly
Thr Ser Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys
Ala Leu Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala
Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn
Pro Ser Phe Leu 355 360 25360PRTArtificial SequenceChimeric CBH II
Polypeptide 25Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Leu Val Gln Thr Leu Ser Glu 50 55 60 Ile Arg Glu Ala Asn
Gln Ala Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Ala
Tyr Leu Glu Cys 145 150 155 160 Ile Asn Tyr Ala Val Thr Gln Leu Asn
Leu Pro Asn Val Ala Met Tyr 165 170 175 Leu Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Gln Asp 180 185 190 Pro Ala Ala Gln Leu
Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro 195 200 205 Arg Ala Leu
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Thr 305 310 315 320 Ser Ala Ala Arg
Tyr Asp Tyr His Cys Gly Leu Ser Asp Ala Leu Thr 325 330 335 Pro Ala
Pro Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu 340 345 350
Leu Ile Asn Ala Asn Pro Pro Phe 355 360 26359PRTArtificial
SequenceChimeric CBH II Polypeptide 26Gly Asn Pro Phe Val Gly Val
Thr Pro Trp Ala Asn Ala Tyr Tyr Ala 1 5 10 15 Ser Glu Val Ser Ser
Leu Ala Ile Pro Ser Leu Thr Gly Ala Met Ala 20 25 30 Thr Ala Ala
Ala Ala Val Ala Lys Val Pro Ser Phe Gln Trp Leu Asp 35 40 45 Arg
Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu Ile 50 55
60 Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe
65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala
Ser Asn 85 90 95 Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn
Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg Ile Arg Glu Ile Leu Ile Ser
Phe Ser Asp Val Arg Thr 115 120 125 Ile Leu Val Ile Glu Pro Asp Ser
Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn Val Pro Lys Cys Ser
Gly Ala Ala Ser Thr Tyr Arg Glu Leu Thr 145 150 155 160 Ile Tyr Ala
Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr Met 165 170 175 Asp
Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro 180 185
190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro Arg
195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala
Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro
Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro
Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp
Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp
Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg
Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val
Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Thr Ser 305 310
315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Ser Asp Ala Leu Thr
Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu
Gln Leu Leu 340 345 350 Ile Asn Ala Asn Pro Pro Phe 355
27358PRTArtificial SequenceChimeric CBH II Polypeptide 27Gly Asn
Pro Phe Ser Gly Val Gln Leu Trp Ala Asn Thr Tyr Tyr Ser 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Ser Pro Glu Leu Ala 20
25 30 Ala Lys Ala Ala Lys Val Ala Glu Val Pro Ser Phe Gln Trp Leu
Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu
Ala Glu Ile 50 55 60 Arg Ala Ala Asn Gln Arg Gly Gly Asn Tyr Ala
Gly Gln Phe Val Val 65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp Cys Ala
Ala Leu Ala Ser Asn Gly Glu 85 90 95 Tyr Ser Ile Ala Asp Gly Gly
Val Ala Lys Tyr Lys Asn Tyr Ile Asp 100 105 110 Arg Ile Arg Glu Ile
Leu Ile Ser Phe Ser Asp Val Arg Thr Ile Leu 115 120 125 Val Ile Glu
Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn Val 130 135 140 Pro
Lys Cys Ser Gly Ala Ala Ser Thr Tyr Lys Glu Leu Thr Val Tyr 145 150
155 160 Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met Asp
Ala 165 170 175 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln
Pro Ala Ala 180 185 190 Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly
Arg Pro Ala Ala Val 195 200 205 Arg Gly Leu Ala Thr Asn Val Ala Asn
Tyr Asn Ala Trp Ser Ile Ala 210 215 220 Ser Pro Pro Ser Tyr Thr Ser
Pro Asn Pro Asn Tyr Asp Glu Lys His 225 230 235 240 Tyr Ile Glu Ala
Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe Asp Ala 245 250 255 Lys Phe
Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr Gly Gln 260 265 270
Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe Gly Val 275
280 285 Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe Val
Trp 290 295 300 Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser
Ser Ala Pro 305 310 315 320 Arg Phe Asp Ser His Cys Ala Leu Pro Asp
Ala Leu Gln Pro Ala Pro 325 330 335 Gln Ala Gly Ala Trp Phe Gln Ala
Tyr Phe Val Gln Leu Leu Thr Asn 340 345 350 Ala Asn Pro Ser Phe Leu
355 28361PRTArtificial SequenceChimeric CBH II Polypeptide 28Gly
Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10
15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu
20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln
Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly
Thr Leu
Ala Glu 50 55 60 Ile Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Pro
Tyr Ala Gly Ile 65 70 75 80 Phe Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser 85 90 95 Asn Gly Glu Trp Ser Ile Ala Asn
Asn Gly Ala Asn Asn Tyr Lys Arg 100 105 110 Tyr Ile Asp Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg 115 120 125 Thr Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met Asn
Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Lys Glu Leu 145 150 155
160 Thr Val Tyr Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr
165 170 175 Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln 180 185 190 Pro Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp
Ala Gly Arg Pro 195 200 205 Ala Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Gly Trp 210 215 220 Asn Ile Thr Ser Pro Pro Ser Tyr
Thr Gln Gly Asn Ala Val Tyr Asn 225 230 235 240 Glu Lys Leu Tyr Ile
His Ala Ile Gly Pro Leu Leu Ala Asn His Gly 245 250 255 Trp Ser Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270 Thr
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275 280
285 Phe Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Ser 305 310 315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala Leu
Pro Asp Ala Leu Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp Phe
Gln Ala Tyr Phe Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro Ser
Phe Leu 355 360 29357PRTArtificial SequenceChimeric CBH II
Polypeptide 29Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala
Tyr Tyr Ala 1 5 10 15 Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu
Thr Gly Ala Met Ala 20 25 30 Thr Ala Ala Ala Ala Val Ala Lys Val
Pro Ser Phe Met Trp Leu Asp 35 40 45 Thr Leu Asp Lys Thr Pro Leu
Met Glu Gln Thr Leu Ala Asp Ile Arg 50 55 60 Thr Ala Asn Lys Asn
Gly Gly Asn Tyr Ala Gly Gln Phe Val Val Tyr 65 70 75 80 Asp Leu Pro
Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu Tyr 85 90 95 Ser
Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys Asn Tyr Ile Asp Arg 100 105
110 Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr Ile Leu Val
115 120 125 Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn
Val Gln 130 135 140 Lys Cys Ser Asn Ala Ala Ser Ala Tyr Leu Glu Cys
Ile Asn Tyr Ala 145 150 155 160 Val Thr Gln Leu Asn Leu Pro Asn Val
Ala Met Tyr Leu Asp Ala Gly 165 170 175 His Ala Gly Trp Leu Gly Trp
Pro Ala Asn Gln Asp Pro Ala Ala Gln 180 185 190 Leu Phe Ala Asn Val
Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu Arg 195 200 205 Gly Leu Ala
Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser Val Ser Ser 210 215 220 Pro
Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys His Tyr 225 230
235 240 Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe Pro Ala
Lys 245 250 255 Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr
Gly Gln Leu 260 265 270 Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr
Gly Phe Gly Val Arg 275 280 285 Pro Thr Ala Asn Thr Gly His Glu Leu
Val Asp Ala Phe Val Trp Val 290 295 300 Lys Pro Gly Gly Glu Ser Asp
Gly Thr Ser Asp Ser Ser Ala Pro Arg 305 310 315 320 Phe Asp Ser His
Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln 325 330 335 Ala Gly
Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala 340 345 350
Asn Pro Ser Phe Leu 355 30361PRTArtificial SequenceChimeric CBH II
Polypeptide 30Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Leu Val Gln Thr Leu Ser Glu 50 55 60 Ile Arg Glu Ala Asn
Gln Ala Gly Ala Asn Pro Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala 100 105
110 Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr
Tyr Lys Glu Leu 145 150 155 160 Thr Val Tyr Ala Leu Lys Gln Leu Asn
Leu Pro His Val Ala Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu
Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro 195 200 205 Ala Ala Val
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Val Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg
Gly 245 250 255 Phe Pro Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg
Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala
Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350
Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360 31359PRTArtificial
SequenceChimeric CBH II Polypeptide 31Gly Asn Pro Phe Val Gly Val
Thr Pro Trp Ala Asn Ala Tyr Tyr Ala 1 5 10 15 Ser Glu Val Ser Ser
Leu Ala Ile Pro Ser Leu Thr Gly Ala Met Ala 20 25 30 Thr Ala Ala
Ala Ala Val Ala Lys Val Pro Ser Phe Gln Trp Leu Asp 35 40 45 Arg
Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu Ser Glu Ile 50 55
60 Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe
65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala
Ser Asn 85 90 95 Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn
Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln
Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val Ile Glu Pro Asp Ser
Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn Val Gln Lys Cys Ser
Asn Ala Ala Ser Thr Tyr Lys Glu Leu Thr 145 150 155 160 Val Tyr Ala
Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr Met 165 170 175 Asp
Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro 180 185
190 Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro Ala
195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala
Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro
Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro
Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp
Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp
Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg
Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val
Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Thr Thr 305 310
315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys
Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile
Gln Leu Leu 340 345 350 Arg Asn Ala Asn Pro Pro Phe 355
32359PRTArtificial SequenceChimeric CBH II Polypeptide 32Gly Asn
Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala Tyr Tyr Ala 1 5 10 15
Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu Thr Gly Ala Met Ala 20
25 30 Thr Ala Ala Ala Ala Val Ala Lys Val Pro Ser Phe Gln Trp Leu
Asp 35 40 45 Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu
Ser Glu Ile 50 55 60 Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Pro
Tyr Ala Gly Ile Phe 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ser Ile Ala Asn
Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg Ile Arg
Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu Thr 145 150
155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr
Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp
Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Val Ser Ser Pro Pro Pro Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe 245 250 255 Pro Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Thr Thr 305 310 315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu
Glu Asp Ala Leu Lys Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe
Asn Glu Tyr Phe Ile Gln Leu Leu 340 345 350 Arg Asn Ala Asn Pro Pro
Phe 355 33359PRTArtificial SequenceChimeric CBH II Polypeptide
33Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala
Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe
Met Trp Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln
Thr Leu Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn
Pro Pro Tyr Ala Gly Ile Phe 65 70 75 80 Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ser Ile
Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg
Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile
Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135
140 Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Ala Tyr Leu Glu Cys Ile
145 150 155 160 Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala
Met Tyr Leu 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Gln Asp Pro 180 185 190 Ala Ala Gln Leu Phe Ala Asn Val Tyr
Lys Asn Ala Ser Ser Pro Arg 195 200 205 Ala Leu Arg Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro
Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His
Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255
Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260
265 270 Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly
Phe 275 280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val
Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly
Thr Ser Asp Thr Thr 305 310 315 320 Ala Ala Arg Tyr Asp Tyr His Cys
Gly Leu Glu Asp Ala Leu Lys Pro 325 330 335 Ala Pro Glu Ala Gly Gln
Trp Phe Asn Glu Tyr Phe Ile Gln Leu Leu 340 345 350 Arg Asn Ala Asn
Pro Pro Phe 355 34361PRTArtificial SequenceChimeric CBH II
Polypeptide 34Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala 100 105
110 Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Ala
Tyr Leu Glu Cys 145 150 155
160 Ile Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr
165 170 175 Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Gln Asp 180 185 190 Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn
Ala Ser Ser Pro 195 200 205 Arg Ala Leu Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Ile Ala Ser Pro Pro Ser Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr Ile
Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly 245 250 255 Phe Asp Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270 Thr
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275 280
285 Phe Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Ser 305 310 315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala Leu
Pro Asp Ala Leu Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp Phe
Gln Ala Tyr Phe Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro Ser
Phe Leu 355 360 35358PRTArtificial SequenceChimeric CBH II
Polypeptide 35Gly Asn Pro Phe Val Gly Val Thr Pro Trp Ala Asn Ala
Tyr Tyr Ala 1 5 10 15 Ser Glu Val Ser Ser Leu Ala Ile Pro Ser Leu
Thr Gly Ala Met Ala 20 25 30 Thr Ala Ala Ala Ala Val Ala Lys Val
Pro Ser Phe Met Trp Leu Asp 35 40 45 Thr Leu Asp Lys Thr Pro Leu
Met Glu Gln Thr Leu Ala Asp Ile Arg 50 55 60 Thr Ala Asn Lys Asn
Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe Val 65 70 75 80 Val Tyr Asp
Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn Gly 85 90 95 Glu
Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr Ile 100 105
110 Asp Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr Ile
115 120 125 Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn
Met Asn 130 135 140 Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg
Glu Leu Thr Ile 145 150 155 160 Tyr Ala Leu Lys Gln Leu Asp Leu Pro
His Val Ala Met Tyr Met Asp 165 170 175 Ala Gly His Ala Gly Trp Leu
Gly Trp Pro Ala Asn Ile Gln Pro Ala 180 185 190 Ala Glu Leu Phe Ala
Lys Ile Tyr Glu Asp Ala Gly Lys Pro Arg Ala 195 200 205 Val Arg Gly
Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser Ile 210 215 220 Ala
Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys 225 230
235 240 His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe
Asp 245 250 255 Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln
Pro Thr Gly 260 265 270 Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys
Gly Thr Gly Phe Gly 275 280 285 Val Arg Pro Thr Ala Asn Thr Gly His
Glu Leu Val Asp Ala Phe Val 290 295 300 Trp Val Lys Pro Gly Gly Glu
Ser Asp Gly Thr Ser Asp Thr Thr Ala 305 310 315 320 Ala Arg Tyr Asp
Tyr His Cys Gly Leu Glu Asp Ala Leu Lys Pro Ala 325 330 335 Pro Glu
Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu Leu Arg 340 345 350
Asn Ala Asn Pro Pro Phe 355 36360PRTArtificial SequenceChimeric CBH
II Polypeptide 36Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn
Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln
Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala
Glu Val Pro Ser Phe Met Trp Leu 35 40 45 Asp Thr Leu Asp Lys Thr
Pro Leu Met Glu Gln Thr Leu Ala Asp Ile 50 55 60 Arg Thr Ala Asn
Lys Asn Gly Ala Asn Pro Gln Tyr Ala Ala Gln Ile 65 70 75 80 Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95
Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100
105 110 Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg
Thr 115 120 125 Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn Met 130 135 140 Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr
Tyr Arg Glu Leu Thr 145 150 155 160 Ile Tyr Ala Leu Lys Gln Leu Asp
Leu Pro His Val Ala Met Tyr Met 165 170 175 Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro 180 185 190 Ala Ala Glu Leu
Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro Arg 195 200 205 Ala Val
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225
230 235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Ser Ser 305 310 315 320 Ala Pro Arg
Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro 325 330 335 Ala
Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu 340 345
350 Thr Asn Ala Asn Pro Ser Phe Leu 355 360 37360PRTArtificial
SequenceChimeric CBH II Polypeptide 37Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp Leu 35 40 45 Asp
Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile 50 55
60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln Tyr Ala Ala Gln Ile
65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala
Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn
Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser
Phe Ser Asp Val Arg Thr 115 120 125 Ile Leu Val Ile Glu Pro Asp Ser
Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn Val Pro Lys Cys Ser
Gly Ala Ala Ser Ala Tyr Leu Glu Cys Ile 145 150 155 160 Asn Tyr Ala
Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu 165 170 175 Asp
Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro 180 185
190 Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg
195 200 205 Ala Leu Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala
Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro
Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro
Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp
Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp
Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg
Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val
Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser Ser 305 310
315 320 Ala Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln
Pro 325 330 335 Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val
Gln Leu Leu 340 345 350 Thr Asn Ala Asn Pro Ser Phe Leu 355 360
38359PRTArtificial SequenceChimeric CBH II Polypeptide 38Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln
Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn
Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Arg Glu Leu Thr 145 150
155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr
Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp
Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Thr Thr 305 310 315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu
Glu Asp Ala Leu Lys Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe
Asn Glu Tyr Phe Ile Gln Leu Leu 340 345 350 Arg Asn Ala Asn Pro Pro
Phe 355 39360PRTArtificial SequenceChimeric CBH II Polypeptide
39Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala
Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe
Met Trp Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln
Thr Leu Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn
Pro Gln Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile
Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg
Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile
Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135
140 Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Arg Glu Leu Thr
145 150 155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala
Met Tyr Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr
Glu Asp Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro
Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His
Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255
Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260
265 270 Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly
Phe 275 280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val
Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly
Thr Ser Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys
Ala Leu Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala
Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn
Pro Ser Phe Leu 355 360 40360PRTArtificial SequenceChimeric CBH II
Polypeptide 40Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Met Trp Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro
Leu Met Glu Gln Thr Leu Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys
Asn Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe 65 70 75 80 Val Val Tyr
Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly
Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 100 105
110 Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr
115 120 125 Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr
Asn Met 130 135 140 Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Ala Tyr
Leu Glu Cys Ile 145 150 155 160 Asn Tyr Ala Val Thr Gln Leu Asn Leu
Pro Asn Val Ala Met Tyr Leu 165 170 175 Asp Ala Gly His Ala Gly Trp
Leu Gly Trp Pro Ala Asn Gln Asp Pro 180 185 190 Ala Ala Gln Leu Phe
Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg 195 200 205 Ala Leu Arg
Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile
Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230
235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly
Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp Thr
Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp Gly
His Trp Cys Asn Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg Pro
Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val Trp
Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser Ser 305 310 315
320 Ala Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro
325 330 335 Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln
Leu Leu 340 345 350 Thr Asn Ala Asn Pro Ser Phe Leu 355 360
41359PRTArtificial SequenceChimeric CBH II Polypeptide 41Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln
Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn
Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg
Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu Thr 145 150
155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr
Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp
Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Val Ser Ser Pro Pro Pro Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe 245 250 255 Pro Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Thr Thr 305 310 315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu
Glu Asp Ala Leu Lys Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe
Asn Glu Tyr Phe Ile Gln Leu Leu 340 345 350 Arg Asn Ala Asn Pro Pro
Phe 355 42360PRTArtificial SequenceChimeric CBH II Polypeptide
42Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1
5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala
Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe
Met Trp Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln
Thr Leu Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn
Pro Pro Tyr Ala Gly Ile Phe 65 70 75 80 Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ser Ile
Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg
Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr 115 120 125 Ile
Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135
140 Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu Thr
145 150 155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala
Met Tyr Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr
Glu Asp Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro
Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His
Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255
Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260
265 270 Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly
Phe 275 280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val
Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly
Thr Ser Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys
Ala Leu Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala
Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn
Pro Ser Phe Leu 355 360 43360PRTArtificial SequenceChimeric CBH II
Polypeptide 43Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Ala
Tyr Leu Glu Cys 145 150 155 160 Ile Asn Tyr Ala Val Thr Gln Leu Asn
Leu Pro Asn Val Ala Met Tyr 165 170 175 Leu Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Gln Asp 180 185 190 Pro Ala Ala Gln Leu
Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro 195 200 205 Arg Ala Leu
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Thr 305 310 315 320 Thr Ala Ala Arg
Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys 325 330 335 Pro Ala
Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu 340 345 350
Leu Arg Asn Ala Asn Pro Pro Phe 355 360 44359PRTArtificial
SequenceChimeric CBH II Polypeptide 44Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp Leu 35 40 45 Asp
Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile 50 55
60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln Tyr Ala Ala Gln Ile
65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala
Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn
Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg Glu Leu Leu Ile Gln
Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val Ile Glu Pro Asp Ser
Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn Val Gln Lys Cys Ser
Asn Ala Ala Ser Ala Tyr Leu Glu Cys Ile 145 150 155 160 Asn Tyr Ala
Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu 165 170 175 Asp
Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro 180 185
190 Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg
195 200 205 Ala Leu Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala
Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro
Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile Glu Ala Phe Ala Pro
Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala Lys Phe Ile Val Asp
Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp
Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg
Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe 290 295 300 Val
Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Thr Thr 305 310
315 320 Ala Ala Arg Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys
Pro 325 330 335 Ala Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile
Gln Leu Leu 340 345 350 Arg Asn Ala Asn Pro Pro Phe 355
45360PRTArtificial SequenceChimeric CBH II Polypeptide 45Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln
Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn
Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Gln Lys Cys Ser Asn Ala Ala Ser Ala Tyr Leu Glu Cys Ile 145 150
155 160 Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr
Leu 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Gln Asp Pro 180 185 190 Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn
Ala Ser Ser Pro Arg 195 200 205 Ala Leu Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys Ala Leu
Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala Trp Phe
Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn Pro Ser
Phe Leu 355 360 46361PRTArtificial SequenceChimeric CBH II
Polypeptide 46Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Ala
Tyr Leu Glu Cys 145 150 155 160 Ile Asn Tyr Ala Val Thr Gln Leu Asn
Leu Pro Asn Val Ala Met Tyr 165 170 175 Leu Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Gln Asp 180 185 190 Pro Ala Ala Gln Leu
Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro 195 200 205 Arg Ala Leu
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg
Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala
Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350
Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360
47360PRTArtificial SequenceChimeric CBH II Polypeptide 47Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Gln
Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ala Ile Ala Asn
Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105 110 Ile Asn Arg Ile Arg
Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Pro Lys Cys Ser Gly Ala Ala Ser Ala Tyr Leu Glu Cys Ile 145 150
155 160 Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr
Leu 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Gln Asp Pro 180 185 190 Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn
Ala Ser Ser Pro Arg 195 200 205 Ala Leu Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Val Ser Ser Pro Pro Pro Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe 245 250 255 Pro Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys Ala Leu
Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala Trp Phe
Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn Pro Ser
Phe Leu 355 360 48361PRTArtificial SequenceChimeric CBH II
Polypeptide 48Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Thr Ile Arg Gln Ile Val Val Glu Tyr Ser Asp Ile Arg
115 120 125 Thr Leu Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Leu Val
Thr Asn 130 135 140 Leu Gly Thr Pro Lys Cys Ala Asn Ala Gln Ser Ala
Tyr Leu Glu Cys 145 150 155 160 Ile Asn Tyr Ala Val Thr Gln Leu Asn
Leu Pro Asn Val Ala Met Tyr 165 170 175 Leu Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Gln Asp 180 185 190 Pro Ala Ala Gln Leu
Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro 195 200 205 Arg Ala Leu
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg
Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala
Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350
Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360 49361PRTArtificial
SequenceChimeric CBH II Polypeptide 49Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp
Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55
60 Ile Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Gln Tyr Ala Ala Gln
65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala
Ala Ser 85 90 95 Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn
Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn Arg Ile Arg Glu Leu Leu Ile
Gln Tyr Ser Asp Ile Arg 115 120 125 Thr Ile Leu Val Ile Glu Pro Asp
Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met Asn Val Gln Lys Cys
Ser Asn Ala Ala Ser Thr Tyr Arg Glu Leu 145 150 155 160 Thr Ile Tyr
Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr 165 170 175 Met
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185
190 Pro Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro
195 200 205 Arg Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn
Ala Trp 210 215 220 Ser Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn
Pro Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala
Pro Leu Leu Arg Asn Gln Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val
Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu
Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275 280 285 Phe Gly Val
Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala 290 295 300 Phe
Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310
315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu
Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe
Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360
50360PRTArtificial SequenceChimeric CBH II Polypeptide 50Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Ala Asn Pro Pro
Tyr Ala Gly Ile Phe 65 70 75 80 Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly Glu Trp Ser Ile Ala Asn
Asn Gly Ala Asn Asn Tyr Lys Arg Tyr 100 105 110 Ile Asp Arg Ile Arg
Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg Thr 115 120 125 Ile Leu Val
Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met 130 135 140 Asn
Val Gln Lys Cys Ser Asn Ala Ala Ser Thr Tyr Arg Glu Leu Thr 145 150
155 160 Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr
Met 165 170 175 Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu Asp
Ala Gly Lys Pro Arg 195 200 205 Ala Val Arg Gly Leu Ala Thr Asn Val
Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Ile Ala Ser Pro Pro Ser Tyr
Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230 235 240 Lys His Tyr Ile
Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe 245 250 255 Asp Ala
Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr 260 265 270
Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe 275
280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala
Phe 290 295 300 Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser
Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe Asp Ser His Cys Ala Leu
Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro Gln Ala Gly Ala Trp Phe
Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350 Thr Asn Ala Asn Pro Ser
Phe Leu 355 360 51361PRTArtificial SequenceChimeric CBH II
Polypeptide 51Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Ala
Tyr Leu Glu Cys 145 150 155 160 Ile Asn Tyr Ala Val Thr Gln Leu Asn
Leu Pro Asn Val Ala Met Tyr 165 170 175 Leu Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Gln Asp 180 185 190 Pro Ala Ala Gln Leu
Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro 195 200 205 Arg Ala Leu
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg
Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala
Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350
Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360 52361PRTArtificial
SequenceChimeric CBH II Polypeptide 52Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp
Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr Leu Ser Glu 50 55
60 Ile Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro Gln Tyr Ala Ala Gln
65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala
Ala Ser 85 90 95 Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn
Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile
Ser Phe Ser Asp Val Arg 115 120 125 Thr Ile Leu Val Ile Glu Pro Asp
Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met Asn Val Pro Lys Cys
Ser Gly Ala Ala Ser Thr Tyr Lys Glu Leu 145 150 155 160 Thr Val Tyr
Ala Leu Lys Gln Leu Asn Leu Pro His Val Ala Met Tyr 165 170 175 Met
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185
190 Pro Ala Ala Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro
195 200 205 Ala Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn
Ala Trp 210 215 220 Ser Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn
Pro Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala
Pro Leu Leu Arg Asn Gln Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val
Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu
Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275 280 285 Phe Gly Val
Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala 290 295 300 Phe
Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310
315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu
Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe
Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360
53361PRTArtificial SequenceChimeric CBH II Polypeptide 53Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp
Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr
Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro
Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg
Asp Cys Ala Ala Ala Ala Ser 85 90
95 Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala
100 105 110 Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp
Val Arg 115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn
Met Val Thr Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala
Ser Thr Tyr Lys Glu Leu 145 150 155 160 Thr Val Tyr Ala Leu Lys Gln
Leu Asn Leu Pro His Val Ala Met Tyr 165 170 175 Met Asp Ala Gly His
Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala
Glu Leu Phe Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro 195 200 205 Ala
Ala Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215
220 Ser Val Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp
225 230 235 240 Glu Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu
Ala Arg Gly 245 250 255 Phe Pro Ala Lys Phe Ile Val Asp Thr Gly Arg
Asn Gly Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp
Cys Asn Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala
Asn Thr Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys
Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala
Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335
Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340
345 350 Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360
54361PRTArtificial SequenceChimeric CBH II Polypeptide 54Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp
Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr
Leu Ser Glu 50 55 60 Ile Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro
Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg
Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn Gly Glu Trp Ala Ile Ala
Asn Asn Gly Val Asn Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn Arg Ile
Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg 115 120 125 Thr Ile Leu
Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met
Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Ala Tyr Leu Glu Cys 145 150
155 160 Ile Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met
Tyr 165 170 175 Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala
Asn Gln Asp 180 185 190 Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys
Asn Ala Ser Ser Pro 195 200 205 Arg Ala Leu Arg Gly Leu Ala Thr Asn
Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Val Ser Ser Pro Pro Pro
Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr
Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly 245 250 255 Phe Pro
Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270
Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275
280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp
Ala 290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr
Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala
Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp
Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro
Ser Phe Leu 355 360 55360PRTArtificial SequenceChimeric CBH II
Polypeptide 55Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Met Trp Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro
Leu Met Glu Gln Thr Leu Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys
Asn Gly Ala Asn Pro Gln Tyr Ala Ala Gln Ile 65 70 75 80 Val Val Tyr
Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn 85 90 95 Gly
Glu Trp Ala Ile Ala Asn Asn Gly Val Asn Asn Tyr Lys Ala Tyr 100 105
110 Ile Asn Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg Thr
115 120 125 Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr
Asn Met 130 135 140 Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr
Lys Glu Leu Thr 145 150 155 160 Val Tyr Ala Leu Lys Gln Leu Asn Leu
Pro His Val Ala Met Tyr Met 165 170 175 Asp Ala Gly His Ala Gly Trp
Leu Gly Trp Pro Ala Asn Ile Gln Pro 180 185 190 Ala Ala Glu Leu Phe
Ala Gln Ile Tyr Arg Asp Ala Gly Arg Pro Ala 195 200 205 Ala Val Arg
Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser 210 215 220 Val
Ser Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu 225 230
235 240 Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly
Phe 245 250 255 Pro Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys
Gln Pro Thr 260 265 270 Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val
Lys Gly Thr Gly Phe 275 280 285 Gly Val Arg Pro Thr Ala Asn Thr Gly
His Glu Leu Val Asp Ala Phe 290 295 300 Val Trp Val Lys Pro Gly Gly
Glu Ser Asp Gly Thr Ser Asp Ser Ser 305 310 315 320 Ala Pro Arg Phe
Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro 325 330 335 Ala Pro
Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu Leu 340 345 350
Thr Asn Ala Asn Pro Ser Phe Leu 355 360 56361PRTArtificial
SequenceChimeric CBH II Polypeptide 56Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp
Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55
60 Ile Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Gln Tyr Ala Ala Gln
65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala
Ala Ser 85 90 95 Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn
Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn Arg Ile Arg Glu Leu Leu Ile
Gln Tyr Ser Asp Ile Arg 115 120 125 Thr Ile Leu Val Ile Glu Pro Asp
Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met Asn Val Gln Lys Cys
Ser Asn Ala Ala Ser Ala Tyr Leu Glu Cys 145 150 155 160 Ile Asn Tyr
Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr 165 170 175 Leu
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp 180 185
190 Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro
195 200 205 Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn
Ala Trp 210 215 220 Ser Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn
Pro Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala
Pro Leu Leu Arg Asn Gln Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val
Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu
Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275 280 285 Phe Gly Val
Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala 290 295 300 Phe
Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310
315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu
Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe
Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360
57361PRTArtificial SequenceChimeric CBH II Polypeptide 57Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp
Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr Leu Leu Val Gln Thr
Leu Ser Glu 50 55 60 Ile Arg Glu Ala Asn Gln Ala Gly Ala Asn Pro
Gln Tyr Ala Ala Gln 65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg
Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn Gly Glu Trp Ala Ile Ala
Asn Asn Gly Val Asn Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn Arg Ile
Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg 115 120 125 Thr Ile Leu
Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met
Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu 145 150
155 160 Thr Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met
Tyr 165 170 175 Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala
Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu Phe Ala Lys Ile Tyr Glu
Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val Arg Gly Leu Ala Thr Asn
Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Val Ser Ser Pro Pro Pro
Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235 240 Glu Lys His Tyr
Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly 245 250 255 Phe Pro
Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265 270
Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly 275
280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp
Ala 290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly Thr
Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg Phe Asp Ser His Cys Ala
Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala Trp
Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn Pro
Ser Phe Leu 355 360 58361PRTArtificial SequenceChimeric CBH II
Polypeptide 58Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Leu Leu Ile Gln Tyr Ser Asp Ile Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Gln Lys Cys Ser Asn Ala Ala Ser Thr
Tyr Arg Glu Leu 145 150 155 160 Thr Ile Tyr Ala Leu Lys Gln Leu Asp
Leu Pro His Val Ala Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu
Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg
Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala
Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350
Leu Thr Asn Ala Asn Pro Ser Phe Leu 355 360 59361PRTArtificial
SequenceChimeric CBH II Polypeptide 59Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp
Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55
60 Ile Arg Ala Ala Asn Gln Arg Gly Ala Asn Pro Gln Tyr Ala Ala Gln
65 70 75 80 Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala
Ala Ser 85 90 95 Asn Gly Glu Trp Ala Ile Ala Asn Asn Gly Val Asn
Asn Tyr Lys Ala 100 105 110 Tyr Ile Asn Arg Ile Arg Glu Ile Leu Ile
Ser Phe Ser Asp Val Arg 115 120 125 Thr Ile Leu Val Ile Glu Pro Asp
Ser Leu Ala Asn Met Val Thr Asn 130 135 140 Met Asn Val Pro Lys Cys
Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu 145 150 155 160 Thr Ile Tyr
Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala Met Tyr 165 170 175 Met
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185
190 Pro Ala Ala Glu Leu Phe Ala
Lys Ile Tyr Glu Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val Arg Gly
Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Val Ser
Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235 240
Glu Lys His Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly 245
250 255 Phe Pro Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln
Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys
Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr Gly His
Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu
Ser Asp Gly Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg Phe Asp
Ser His Cys Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala Pro Gln
Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350 Leu Thr
Asn Ala Asn Pro Ser Phe Leu 355 360 60358PRTArtificial
SequenceChimeric CBH II Polypeptide 60Gly Asn Pro Phe Glu Gly Val
Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr
Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala
Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp Leu 35 40 45 Asp
Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu Ala Asp Ile 50 55
60 Arg Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala Gly Gln Phe Val Val
65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn
Gly Glu 85 90 95 Tyr Ser Ile Ala Asp Gly Gly Val Ala Lys Tyr Lys
Asn Tyr Ile Asp 100 105 110 Thr Ile Arg Gln Ile Val Val Glu Tyr Ser
Asp Ile Arg Thr Leu Leu 115 120 125 Val Ile Glu Pro Asp Ser Leu Ala
Asn Leu Val Thr Asn Leu Gly Thr 130 135 140 Pro Lys Cys Ala Asn Ala
Gln Ser Ala Tyr Leu Glu Cys Ile Asn Tyr 145 150 155 160 Ala Val Thr
Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu Asp Ala 165 170 175 Gly
His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp Pro Ala Ala 180 185
190 Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser Pro Arg Ala Leu
195 200 205 Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser
Val Ser 210 215 220 Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr
Asp Glu Lys His 225 230 235 240 Tyr Ile Glu Ala Phe Arg Pro Leu Leu
Glu Ala Arg Gly Phe Pro Ala 245 250 255 Lys Phe Ile Val Asp Thr Gly
Arg Asn Gly Lys Gln Pro Thr Gly Gln 260 265 270 Leu Glu Trp Gly His
Trp Cys Asn Val Lys Gly Thr Gly Phe Gly Val 275 280 285 Arg Pro Thr
Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe Val Trp 290 295 300 Val
Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser Ser Ala Pro 305 310
315 320 Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala Leu Gln Pro Ala
Pro 325 330 335 Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe Val Gln Leu
Leu Thr Asn 340 345 350 Ala Asn Pro Ser Phe Leu 355
61358PRTArtificial SequenceChimeric CBH II Polypeptide 61Gly Asn
Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10 15
Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu 20
25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Met Trp
Leu 35 40 45 Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr Leu
Ala Asp Ile 50 55 60 Arg Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala
Gly Gln Phe Val Val 65 70 75 80 Tyr Asp Leu Pro Asp Arg Asp Cys Ala
Ala Leu Ala Ser Asn Gly Glu 85 90 95 Tyr Ser Ile Ala Asp Gly Gly
Val Ala Lys Tyr Lys Asn Tyr Ile Asp 100 105 110 Thr Ile Arg Gln Ile
Val Val Glu Tyr Ser Asp Ile Arg Thr Leu Leu 115 120 125 Val Ile Glu
Pro Asp Ser Leu Ala Asn Leu Val Thr Asn Leu Gly Thr 130 135 140 Pro
Lys Cys Ala Asn Ala Gln Ser Ala Tyr Leu Glu Cys Ile Asn Tyr 145 150
155 160 Ala Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met Tyr Leu Asp
Ala 165 170 175 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln Asp
Pro Ala Ala 180 185 190 Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser
Ser Pro Arg Ala Leu 195 200 205 Arg Gly Leu Ala Thr Asn Val Ala Asn
Tyr Asn Ala Trp Ser Ile Ala 210 215 220 Ser Pro Pro Ser Tyr Thr Ser
Pro Asn Pro Asn Tyr Asp Glu Lys His 225 230 235 240 Tyr Ile Glu Ala
Phe Ala Pro Leu Leu Arg Asn Gln Gly Phe Asp Ala 245 250 255 Lys Phe
Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro Thr Gly Gln 260 265 270
Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly Phe Gly Val 275
280 285 Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val Asp Ala Phe Val
Trp 290 295 300 Val Lys Pro Gly Gly Glu Ser Asp Gly Thr Ser Asp Ser
Ser Ala Pro 305 310 315 320 Arg Phe Asp Ser His Cys Ala Leu Pro Asp
Ala Leu Gln Pro Ala Pro 325 330 335 Gln Ala Gly Ala Trp Phe Gln Ala
Tyr Phe Val Gln Leu Leu Thr Asn 340 345 350 Ala Asn Pro Ser Phe Leu
355 62361PRTArtificial SequenceChimeric CBH II Polypeptide 62Gly
Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn Tyr Tyr Arg 1 5 10
15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile Thr Asp Pro Ala Leu
20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu Val Pro Ser Phe Gln
Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp Thr Leu Phe Ser Gly
Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn Gln Arg Gly Ala Asn
Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn Gly Glu Trp Ser Ile
Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105 110 Tyr Ile Asp Arg
Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg 115 120 125 Thr Ile
Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn 130 135 140
Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu Leu 145
150 155 160 Thr Ile Tyr Ala Leu Lys Gln Leu Asp Leu Pro His Val Ala
Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu Phe Ala Lys Ile Tyr
Glu Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val Arg Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser Ile Ala Ser Pro Pro
Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230 235 240 Glu Lys His
Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln Gly 245 250 255 Phe
Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly Lys Gln Pro 260 265
270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn Val Lys Gly Thr Gly
275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr Gly His Glu Leu Val
Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly Gly Glu Ser Asp Gly
Thr Ser Asp Ser 305 310 315 320 Ser Ala Pro Arg Phe Asp Ser His Cys
Ala Leu Pro Asp Ala Leu Gln 325 330 335 Pro Ala Pro Gln Ala Gly Ala
Trp Phe Gln Ala Tyr Phe Val Gln Leu 340 345 350 Leu Thr Asn Ala Asn
Pro Ser Phe Leu 355 360 63360PRTArtificial SequenceChimeric CBH II
Polypeptide 63Gly Asn Pro Phe Glu Gly Val Gln Leu Trp Ala Asn Asn
Tyr Tyr Arg 1 5 10 15 Ser Glu Val His Thr Leu Ala Ile Pro Gln Ile
Thr Asp Pro Ala Leu 20 25 30 Arg Ala Ala Ala Ser Ala Val Ala Glu
Val Pro Ser Phe Gln Trp Leu 35 40 45 Asp Arg Asn Val Thr Val Asp
Thr Leu Phe Ser Gly Thr Leu Ala Glu 50 55 60 Ile Arg Ala Ala Asn
Gln Arg Gly Ala Asn Pro Pro Tyr Ala Gly Ile 65 70 75 80 Phe Val Val
Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser 85 90 95 Asn
Gly Glu Trp Ser Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Arg 100 105
110 Tyr Ile Asp Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp Val Arg
115 120 125 Thr Ile Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val
Thr Asn 130 135 140 Met Asn Val Pro Lys Cys Ser Gly Ala Ala Ser Thr
Tyr Arg Glu Leu 145 150 155 160 Thr Ile Tyr Ala Leu Lys Gln Leu Asp
Leu Pro His Val Ala Met Tyr 165 170 175 Met Asp Ala Gly His Ala Gly
Trp Leu Gly Trp Pro Ala Asn Ile Gln 180 185 190 Pro Ala Ala Glu Leu
Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro 195 200 205 Arg Ala Val
Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp 210 215 220 Ser
Ile Ala Ser Pro Pro Ser Tyr Thr Ser Pro Asn Pro Asn Tyr Asp 225 230
235 240 Glu Lys His Tyr Ile Glu Ala Phe Ala Pro Leu Leu Arg Asn Gln
Gly 245 250 255 Phe Asp Ala Lys Phe Ile Val Asp Thr Gly Arg Asn Gly
Lys Gln Pro 260 265 270 Thr Gly Gln Leu Glu Trp Gly His Trp Cys Asn
Val Lys Gly Thr Gly 275 280 285 Phe Gly Val Arg Pro Thr Ala Asn Thr
Gly His Glu Leu Val Asp Ala 290 295 300 Phe Val Trp Val Lys Pro Gly
Gly Glu Ser Asp Gly Thr Ser Asp Thr 305 310 315 320 Thr Ala Ala Arg
Tyr Asp Tyr His Cys Gly Leu Glu Asp Ala Leu Lys 325 330 335 Pro Ala
Pro Glu Ala Gly Gln Trp Phe Asn Glu Tyr Phe Ile Gln Leu 340 345 350
Leu Arg Asn Ala Asn Pro Pro Phe 355 360 64447PRTTrichoderma reesei
64Gln Ala Cys Ser Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1
5 10 15 Gly Pro Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn
Asp 20 25 30 Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser
Ser Ser Thr 35 40 45 Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro
Thr Thr Ser Arg Ser 50 55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser
Thr Thr Thr Arg Val Pro Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr
Tyr Ser Gly Asn Pro Phe Val Gly Val 85 90 95 Thr Pro Trp Ala Asn
Ala Tyr Tyr Ala Ser Glu Val Ser Ser Leu Ala 100 105 110 Ile Pro Ser
Leu Thr Gly Ala Met Ala Thr Ala Ala Ala Ala Val Ala 115 120 125 Lys
Val Pro Ser Phe Met Trp Leu Asp Thr Leu Asp Lys Thr Pro Leu 130 135
140 Met Glu Gln Thr Leu Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly
145 150 155 160 Asn Tyr Ala Gly Gln Phe Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys 165 170 175 Ala Ala Leu Ala Ser Asn Gly Glu Tyr Ser Ile
Ala Asp Gly Gly Val 180 185 190 Ala Lys Tyr Lys Asn Tyr Ile Asp Thr
Ile Arg Gln Ile Val Val Glu 195 200 205 Tyr Ser Asp Ile Arg Thr Leu
Leu Val Ile Glu Pro Asp Ser Leu Ala 210 215 220 Asn Leu Val Thr Asn
Leu Gly Thr Pro Lys Cys Ala Asn Ala Gln Ser 225 230 235 240 Ala Tyr
Leu Glu Cys Ile Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro 245 250 255
Asn Val Ala Met Tyr Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp 260
265 270 Pro Ala Asn Gln Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr
Lys 275 280 285 Asn Ala Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr
Asn Val Ala 290 295 300 Asn Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro
Ser Tyr Thr Gln Gly 305 310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu
Tyr Ile His Ala Ile Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp
Ser Asn Ala Phe Phe Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys
Gln Pro Thr Gly Gln Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val
Ile Gly Thr Gly Phe Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380
Asp Ser Leu Leu Asp Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385
390 395 400 Asp Gly Thr Ser Asp Ser Ser Ala Pro Arg Phe Asp Ser His
Cys Ala 405 410 415 Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly
Ala Trp Phe Gln 420 425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala
Asn Pro Ser Phe Leu 435 440 445 65453PRTHumicola insolens 65Ala Ser
Cys Ala Pro Thr Trp Gly Gln Cys Gly Gly Ile Gly Phe Asn 1 5 10 15
Gly Pro Thr Cys Cys Gln Ser Gly Ser Thr Cys Val Lys Gln Asn Asp 20
25 30 Trp Tyr Ser Gln Cys Leu Pro Gly Ser Gln Val Thr Thr Thr Ser
Thr 35 40 45 Thr Ser Thr Ser Ser Ser Ser Thr Thr Ser Arg Ala Thr
Ser Thr Thr 50 55 60 Ser Thr Gly Gly Val Thr Ser Ile Thr Thr Ala
Pro Thr Arg Thr Val 65 70 75 80 Thr Ile Pro Gly Gly Ala Thr Thr Thr
Ala Ser Tyr Asn Gly Asn Pro 85 90 95 Phe Glu Gly Val Gln Leu Trp
Ala Asn Asn Tyr Tyr Arg Ser Glu Val 100 105 110 His Thr Leu Ala Ile
Pro Gln Ile Thr Asp Pro Ala Leu Arg Ala Ala 115 120 125 Ala Ser Ala
Val Ala Glu Val Pro Ser Phe Gln Trp Leu Asp Arg Asn 130 135 140 Val
Thr Val Asp Thr Leu Leu Val Glu Thr Leu Ser Glu Ile Arg Ala 145 150
155 160 Ala Asn Gln Ala Gly Ala Asn Pro Pro Tyr Ala Ala Gln Ile Val
Val 165 170 175 Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser
Asn Gly Glu 180 185 190 Trp Ala Ile Ala Asn Asn Gly Ala Asn Asn Tyr
Lys Gly Tyr Ile Asn 195 200 205 Arg Ile Arg Glu Ile Leu Ile Ser Phe
Ser Asp Val Arg Thr Ile Leu 210
215 220 Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Asn
Val 225 230 235 240 Ala Lys Cys Ser Gly Ala Ala Ser Thr Tyr Arg Glu
Leu Thr Ile Tyr 245 250 255 Ala Leu Lys Gln Leu Asp Leu Pro His Val
Ala Met Tyr Met Asp Ala 260 265 270 Gly His Ala Gly Trp Leu Gly Trp
Pro Ala Asn Ile Gln Pro Ala Ala 275 280 285 Glu Leu Phe Ala Lys Ile
Tyr Glu Asp Ala Gly Lys Pro Arg Ala Val 290 295 300 Arg Gly Leu Ala
Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser Ile Ser 305 310 315 320 Ser
Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr Asp Glu Lys His 325 330
335 Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu Ala Arg Gly Phe Pro Ala
340 345 350 Gln Phe Ile Val Asp Gln Gly Arg Ser Gly Lys Gln Pro Thr
Gly Gln 355 360 365 Lys Glu Trp Gly His Trp Cys Asn Ala Ile Gly Thr
Gly Phe Gly Met 370 375 380 Arg Pro Thr Ala Asn Thr Gly His Gln Tyr
Val Asp Ala Phe Val Trp 385 390 395 400 Val Lys Pro Gly Gly Glu Cys
Asp Gly Thr Ser Asp Thr Thr Ala Ala 405 410 415 Arg Tyr Asp Tyr His
Cys Gly Leu Glu Asp Ala Leu Lys Pro Ala Pro 420 425 430 Glu Ala Gly
Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu Arg Asn 435 440 445 Ala
Asn Pro Pro Phe 450 66440PRTPhanerochaete chrysosporium 66Ala Ser
Ser Glu Trp Gly Gln Cys Gly Gly Ile Gly Trp Thr Gly Pro 1 5 10 15
Thr Thr Cys Val Ser Gly Thr Thr Cys Thr Val Leu Asn Pro Tyr Tyr 20
25 30 Ser Gln Cys Leu Pro Gly Ser Ala Val Thr Thr Thr Ser Val Ile
Thr 35 40 45 Ser His Ser Ser Ser Val Ser Ser Val Ser Ser His Ser
Gly Ser Ser 50 55 60 Thr Ser Thr Ser Ser Pro Thr Gly Pro Thr Gly
Thr Asn Pro Pro Pro 65 70 75 80 Pro Pro Ser Ala Asn Asn Pro Trp Thr
Gly Phe Gln Ile Phe Leu Ser 85 90 95 Pro Tyr Tyr Ala Asn Glu Val
Ala Ala Ala Ala Lys Gln Ile Thr Asp 100 105 110 Pro Thr Leu Ser Ser
Lys Ala Ala Ser Val Ala Asn Ile Pro Thr Phe 115 120 125 Thr Trp Leu
Asp Ser Val Ala Lys Ile Pro Asp Leu Gly Thr Tyr Leu 130 135 140 Ala
Ser Ala Ser Ala Leu Gly Lys Ser Thr Gly Thr Lys Gln Leu Val 145 150
155 160 Gln Ile Val Ile Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Lys
Ala 165 170 175 Ser Asn Gly Glu Phe Ser Ile Ala Asn Asn Gly Gln Ala
Asn Tyr Glu 180 185 190 Asn Tyr Ile Asp Gln Ile Val Ala Gln Ile Gln
Gln Phe Pro Asp Val 195 200 205 Arg Val Val Ala Val Ile Glu Pro Asp
Ser Leu Ala Asn Leu Val Thr 210 215 220 Asn Leu Asn Val Gln Lys Cys
Ala Asn Ala Lys Thr Thr Tyr Leu Ala 225 230 235 240 Cys Val Asn Tyr
Ala Leu Thr Asn Leu Ala Lys Val Gly Val Tyr Met 245 250 255 Tyr Met
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Leu 260 265 270
Ser Pro Ala Ala Gln Leu Phe Thr Gln Val Trp Gln Asn Ala Gly Lys 275
280 285 Ser Pro Phe Ile Lys Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn
Ala 290 295 300 Leu Gln Ala Ala Ser Pro Asp Pro Ile Thr Gln Gly Asn
Pro Asn Tyr 305 310 315 320 Asp Glu Ile His Tyr Ile Asn Ala Leu Ala
Pro Leu Leu Gln Gln Ala 325 330 335 Gly Trp Asp Ala Thr Phe Ile Val
Asp Gln Gly Arg Ser Gly Val Gln 340 345 350 Asn Ile Arg Gln Gln Trp
Gly Asp Trp Cys Asn Ile Lys Gly Ala Gly 355 360 365 Phe Gly Thr Arg
Pro Thr Thr Asn Thr Gly Ser Gln Phe Ile Asp Ser 370 375 380 Ile Val
Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asn Ser 385 390 395
400 Ser Ser Pro Arg Tyr Asp Ser Thr Cys Ser Leu Pro Asp Ala Ala Gln
405 410 415 Pro Ala Pro Glu Ala Gly Thr Trp Phe Gln Ala Tyr Phe Gln
Thr Leu 420 425 430 Val Ser Ala Ala Asn Pro Pro Leu 435 440
67447PRTTrichoderma reesei 67Gln Ala Cys Ser Ser Val Trp Gly Gln
Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro Thr Cys Cys Ala Ser
Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 Tyr Tyr Ser Gln Cys
Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40 45 Arg Ala Ala
Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 50 55 60 Ser
Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro 65 70
75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly Asn Pro Phe Val Gly
Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala Ser Glu Val Ser
Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala Met Ala Thr Ala
Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe Met Trp Leu Asp
Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln Thr Leu Ala Asp
Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160 Asn Tyr Ala Gly
Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165 170 175 Ala Ala
Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val 180 185 190
Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val Val Glu 195
200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu Pro Asp Ser Leu
Ala 210 215 220 Asn Leu Val Thr Asn Leu Gly Thr Pro Lys Cys Ala Asn
Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile Asn Tyr Ala Val
Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met Tyr Leu Asp Ala
Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro Ala Asn Gln Asp Pro
Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280 285 Asn Ala Ser Ser
Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala 290 295 300 Asn Tyr
Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln Gly 305 310 315
320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile His Ala Ile Gly Pro Leu
325 330 335 Leu Ala Asn His Gly Trp Ser Asn Ala Phe Phe Ile Thr Asp
Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro Thr Gly Gln Gln Gln Trp
Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly Thr Gly Phe Gly Ile Arg
Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser Leu Leu Asp Ser Phe Val
Trp Val Lys Pro Gly Gly Glu Cys 385 390 395 400 Asp Gly Thr Ser Asp
Ser Ser Ala Pro Arg Phe Asp Pro His Cys Ala 405 410 415 Leu Pro Asp
Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln 420 425 430 Ala
Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro Ser Phe Leu 435 440 445
68447PRTTrichoderma reesei 68Gln Ala Cys Ser Ser Val Trp Gly Gln
Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro Thr Cys Cys Ala Ser
Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 Tyr Tyr Ser Gln Cys
Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40 45 Arg Ala Ala
Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 50 55 60 Ser
Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro 65 70
75 80 Val Glu Ser Gly Thr Ala Thr Tyr Ser Gly Asn Pro Phe Val Gly
Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala Ser Glu Val Ser
Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala Met Ala Thr Ala
Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe Met Trp Leu Asp
Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln Thr Leu Ala Asp
Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160 Asn Tyr Ala Gly
Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165 170 175 Ala Ala
Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val 180 185 190
Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val Val Glu 195
200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu Pro Asp Ser Leu
Ala 210 215 220 Asn Leu Val Thr Asn Leu Ser Thr Pro Lys Cys Ala Asn
Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile Asn Tyr Ala Val
Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met Tyr Leu Asp Ala
Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro Ala Asn Gln Asp Pro
Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280 285 Asn Ala Ser Ser
Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala 290 295 300 Ser Tyr
Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln Gly 305 310 315
320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile His Ala Ile Gly Pro Leu
325 330 335 Leu Ala Asn His Gly Trp Ser Asn Ala Phe Phe Ile Thr Asp
Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro Thr Gly Gln Gln Gln Trp
Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly Thr Gly Phe Gly Ile Arg
Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser Leu Leu Asp Ser Phe Val
Trp Val Lys Pro Gly Gly Glu Cys 385 390 395 400 Asp Gly Thr Ser Asp
Ser Ser Ala Pro Gln Phe Asp Pro His Cys Ala 405 410 415 Leu Pro Asp
Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln 420 425 430 Ala
Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro Ser Phe Leu 435 440 445
69447PRTTrichoderma reesei 69Gln Ala Cys Ser Ser Val Trp Gly Gln
Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro Thr Cys Cys Ala Ser
Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 Tyr Tyr Ser Gln Cys
Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40 45 Arg Ala Ala
Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 50 55 60 Ser
Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro 65 70
75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly Asn Pro Phe Val Gly
Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala Ser Glu Val Ser
Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala Met Ala Thr Ala
Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe Met Trp Leu Asp
Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln Thr Leu Ala Asp
Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160 Asn Tyr Ala Gly
Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165 170 175 Ala Ala
Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val 180 185 190
Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val Val Glu 195
200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu Pro Asp Ser Leu
Ala 210 215 220 Asn Leu Val Thr Asn Leu Ser Thr Pro Lys Cys Ala Asn
Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile Asn Tyr Ala Val
Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met Tyr Leu Asp Ala
Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro Ala Asn Gln Asp Pro
Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280 285 Asn Ala Ser Ser
Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala 290 295 300 Asn Tyr
Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln Gly 305 310 315
320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile His Ala Ile Gly Pro Leu
325 330 335 Leu Ala Asn His Gly Trp Ser Asn Ala Phe Phe Ile Thr Asp
Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro Thr Gly Gln Gln Gln Trp
Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly Thr Gly Phe Gly Ile Arg
Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser Leu Leu Asp Ser Phe Val
Trp Val Lys Pro Gly Gly Glu Cys 385 390 395 400 Asp Gly Thr Ser Asp
Ser Ser Ala Pro Arg Phe Asp Pro His Cys Ala 405 410 415 Leu Pro Asp
Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln 420 425 430 Ala
Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro Ser Phe Leu 435 440 445
70447PRTTrichoderma reesei 70Gln Ala Cys Ser Ser Val Trp Gly Gln
Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro Thr Cys Cys Ala Ser
Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 Tyr Tyr Ser Gln Cys
Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40 45 Arg Ala Ala
Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 50 55 60 Ser
Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro 65 70
75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly Asn Pro Phe Val Gly
Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala Ser Glu Val Ser
Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala Met Ala Thr Ala
Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe Met Trp Leu Asp
Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln Thr Leu Ala Asp
Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160 Asn Tyr Ala Gly
Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165 170 175 Ala Ala
Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val 180 185 190
Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val Val Glu 195
200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu Pro Asp Ser Leu
Ala 210 215 220 Asn Leu Val Thr Asn Leu Gly Thr Pro Lys Cys Ala Asn
Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile Asn Tyr Ala Val
Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met Tyr Leu Asp Ala
Gly His Ala Gly Trp Leu Gly Trp 260 265
270 Pro Ala Asn Gln Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys
275 280 285 Asn Ala Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn
Val Ala 290 295 300 Ser Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser
Tyr Thr Gln Gly 305 310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr
Ile His Ala Ile Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp Ser
Asn Ala Phe Phe Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys Gln
Pro Thr Gly Gln Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val Ile
Gly Thr Gly Phe Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380 Asp
Ser Leu Leu Asp Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385 390
395 400 Asp Gly Thr Ser Asp Ser Ser Ala Pro Arg Phe Asp Pro His Cys
Ala 405 410 415 Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala
Trp Phe Gln 420 425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn
Pro Ser Phe Leu 435 440 445 71447PRTTrichoderma reesei 71Gln Ala
Cys Ser Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1 5 10 15
Gly Pro Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 20
25 30 Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser
Thr 35 40 45 Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr
Ser Arg Ser 50 55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr
Thr Arg Val Pro Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser
Gly Asn Pro Phe Val Gly Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr
Tyr Ala Ser Glu Val Ser Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr
Gly Ala Met Ala Thr Ala Ala Ala Ala Val Ala 115 120 125 Lys Val Pro
Ser Phe Met Trp Leu Asp Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met
Glu Gln Thr Leu Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150
155 160 Asn Tyr Ala Gly Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp
Cys 165 170 175 Ala Ala Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp
Gly Gly Val 180 185 190 Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg
Gln Ile Val Val Glu 195 200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val
Ile Glu Pro Asp Ser Leu Ala 210 215 220 Asn Leu Val Thr Asn Leu Gly
Thr Pro Lys Cys Ala Asn Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu
Cys Ile Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val
Ala Met Tyr Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp 260 265 270
Pro Ala Asn Gln Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275
280 285 Asn Ala Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val
Ala 290 295 300 Asn Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr
Thr Gln Gly 305 310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile
His Ala Ile Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp Ser Asn
Ala Phe Phe Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro
Thr Gly Gln Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly
Thr Gly Phe Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser
Leu Leu Asp Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385 390 395
400 Asp Gly Thr Ser Asp Ser Ser Ala Pro Gln Phe Asp Pro His Cys Ala
405 410 415 Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp
Phe Gln 420 425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro
Ser Phe Leu 435 440 445 72447PRTTrichoderma reesei 72Gln Ala Cys
Ser Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly
Pro Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25
30 Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr
35 40 45 Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser
Arg Ser 50 55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr
Arg Val Pro Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly
Asn Pro Phe Val Gly Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr
Ala Ser Glu Val Ser Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly
Ala Met Ala Thr Ala Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser
Phe Met Trp Leu Asp Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu
Gln Thr Leu Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155
160 Asn Tyr Ala Gly Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys
165 170 175 Ala Ala Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly
Gly Val 180 185 190 Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln
Ile Val Val Glu 195 200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile
Glu Pro Asp Ser Leu Ala 210 215 220 Asn Leu Val Thr Asn Leu Ser Thr
Pro Lys Cys Ala Asn Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys
Ile Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala
Met Tyr Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro
Ala Asn Gln Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280
285 Asn Ala Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala
290 295 300 Ser Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr
Gln Gly 305 310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile His
Ala Ile Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp Ser Asn Ala
Phe Phe Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro Thr
Gly Gln Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly Thr
Gly Phe Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser Leu
Leu Asp Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385 390 395 400
Asp Gly Thr Ser Asp Ser Ser Ala Pro Arg Phe Asp Pro His Cys Ala 405
410 415 Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe
Gln 420 425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro Ser
Phe Leu 435 440 445 73447PRTTrichoderma reesei 73Gln Ala Cys Ser
Ser Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro
Thr Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30
Tyr Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35
40 45 Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg
Ser 50 55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg
Val Pro Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly Asn
Pro Phe Val Gly Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala
Ser Glu Val Ser Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala
Met Ala Thr Ala Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe
Met Trp Leu Asp Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln
Thr Leu Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160
Asn Tyr Ala Gly Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165
170 175 Ala Ala Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly
Val 180 185 190 Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile
Val Val Glu 195 200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu
Pro Asp Ser Leu Ala 210 215 220 Asn Leu Val Thr Asn Leu Ser Thr Pro
Lys Cys Ala Asn Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile
Asn Tyr Ala Val Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met
Tyr Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro Ala
Asn Gln Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280 285
Asn Ala Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala 290
295 300 Asn Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln
Gly 305 310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile His Ala
Ile Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp Ser Asn Ala Phe
Phe Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro Thr Gly
Gln Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly Thr Gly
Phe Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser Leu Leu
Asp Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385 390 395 400 Asp
Gly Thr Ser Asp Ser Ser Ala Pro Gln Phe Asp Pro His Cys Ala 405 410
415 Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln
420 425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro Ser Phe
Leu 435 440 445 74447PRTTrichoderma reesei 74Gln Ala Cys Ser Ser
Val Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro Thr
Cys Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 Tyr
Tyr Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40
45 Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser
50 55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val
Pro Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly Asn Pro
Phe Val Gly Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala Ser
Glu Val Ser Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala Met
Ala Thr Ala Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe Met
Trp Leu Asp Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln Thr
Leu Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160 Asn
Tyr Ala Gly Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165 170
175 Ala Ala Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val
180 185 190 Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val
Val Glu 195 200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu Pro
Asp Ser Leu Ala 210 215 220 Asn Leu Val Thr Asn Leu Gly Thr Pro Lys
Cys Ala Asn Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile Asn
Tyr Ala Val Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met Tyr
Leu Asp Ala Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro Ala Asn
Gln Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280 285 Asn
Ala Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala 290 295
300 Ser Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln Gly
305 310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile His Ala Ile
Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp Ser Asn Ala Phe Phe
Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro Thr Gly Gln
Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly Thr Gly Phe
Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser Leu Leu Asp
Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385 390 395 400 Asp Gly
Thr Ser Asp Ser Ser Ala Pro Gln Phe Asp Pro His Cys Ala 405 410 415
Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln 420
425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro Ser Phe Leu
435 440 445 75447PRTTrichoderma reesei 75Gln Ala Cys Ser Ser Val
Trp Gly Gln Cys Gly Gly Gln Asn Trp Ser 1 5 10 15 Gly Pro Thr Cys
Cys Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp 20 25 30 Tyr Tyr
Ser Gln Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr 35 40 45
Arg Ala Ala Ser Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser 50
55 60 Ser Ser Ala Thr Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro
Pro 65 70 75 80 Val Gly Ser Gly Thr Ala Thr Tyr Ser Gly Asn Pro Phe
Val Gly Val 85 90 95 Thr Pro Trp Ala Asn Ala Tyr Tyr Ala Ser Glu
Val Ser Ser Leu Ala 100 105 110 Ile Pro Ser Leu Thr Gly Ala Met Ala
Thr Ala Ala Ala Ala Val Ala 115 120 125 Lys Val Pro Ser Phe Met Trp
Leu Asp Thr Leu Asp Lys Thr Pro Leu 130 135 140 Met Glu Gln Thr Leu
Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly 145 150 155 160 Asn Tyr
Ala Gly Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys 165 170 175
Ala Ala Leu Ala Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val 180
185 190 Ala Lys Tyr Lys Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val Val
Glu 195 200 205 Tyr Ser Asp Ile Arg Thr Leu Leu Val Ile Glu Pro Asp
Ser Leu Ala 210 215 220 Asn Leu Val Thr Asn Leu Ser Thr Pro Lys Cys
Ala Asn Ala Gln Ser 225 230 235 240 Ala Tyr Leu Glu Cys Ile Asn Tyr
Ala Val Thr Gln Leu Asn Leu Pro 245 250 255 Asn Val Ala Met Tyr Leu
Asp Ala Gly His Ala Gly Trp Leu Gly Trp 260 265 270 Pro Ala Asn Gln
Asp Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys 275 280 285 Asn Ala
Ser Ser Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala 290 295 300
Ser Tyr Asn Gly Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln Gly 305
310 315 320 Asn Ala Val Tyr Asn Glu Lys Leu Tyr Ile
His Ala Ile Gly Pro Leu 325 330 335 Leu Ala Asn His Gly Trp Ser Asn
Ala Phe Phe Ile Thr Asp Gln Gly 340 345 350 Arg Ser Gly Lys Gln Pro
Thr Gly Gln Gln Gln Trp Gly Asp Trp Cys 355 360 365 Asn Val Ile Gly
Thr Gly Phe Gly Ile Arg Pro Ser Ala Asn Thr Gly 370 375 380 Asp Ser
Leu Leu Asp Ser Phe Val Trp Val Lys Pro Gly Gly Glu Cys 385 390 395
400 Asp Gly Thr Ser Asp Ser Ser Ala Pro Gln Phe Asp Pro His Cys Ala
405 410 415 Leu Pro Asp Ala Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp
Phe Gln 420 425 430 Ala Tyr Phe Val Gln Leu Leu Thr Asn Ala Asn Pro
Ser Phe Leu 435 440 445 76453PRTHumicola insolens 76Ala Ser Cys Ala
Pro Thr Trp Gly Gln Cys Gly Gly Ile Gly Phe Asn 1 5 10 15 Gly Pro
Thr Cys Cys Gln Ser Gly Ser Thr Cys Val Lys Gln Asn Asp 20 25 30
Trp Tyr Ser Gln Cys Leu Pro Gly Ser Gln Val Thr Thr Thr Ser Thr 35
40 45 Thr Ser Thr Ser Ser Ser Ser Thr Thr Ser Arg Ala Thr Ser Thr
Thr 50 55 60 Ser Thr Gly Gly Val Thr Ser Ile Thr Thr Ala Pro Thr
Arg Thr Val 65 70 75 80 Thr Ile Pro Gly Gly Ala Thr Thr Thr Ala Ser
Tyr Asn Gly Asn Pro 85 90 95 Phe Glu Gly Val Gln Leu Trp Ala Asn
Asn Tyr Tyr Arg Ser Glu Val 100 105 110 His Thr Leu Ala Ile Pro Gln
Ile Thr Asp Pro Ala Leu Arg Ala Ala 115 120 125 Ala Ser Ala Val Ala
Glu Val Pro Ser Phe Gln Trp Leu Asp Arg Asn 130 135 140 Val Thr Val
Asp Thr Leu Leu Val Glu Thr Leu Ser Glu Ile Arg Ala 145 150 155 160
Ala Asn Gln Ala Gly Ala Asn Pro Pro Tyr Ala Ala Gln Ile Val Val 165
170 175 Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn Gly
Glu 180 185 190 Trp Ala Ile Ala Asn Asn Gly Ala Asn Asn Tyr Lys Gly
Tyr Ile Asn 195 200 205 Arg Ile Arg Glu Ile Leu Ile Ser Phe Ser Asp
Val Arg Thr Ile Leu 210 215 220 Val Ile Glu Pro Asp Ser Leu Ala Asn
Met Val Thr Asn Met Asn Val 225 230 235 240 Ala Lys Cys Ser Gly Ala
Ala Ser Thr Tyr Arg Glu Leu Thr Ile Tyr 245 250 255 Ala Leu Lys Gln
Leu Asp Leu Pro His Val Ala Met Tyr Met Asp Ala 260 265 270 Gly His
Ala Gly Trp Leu Gly Trp Pro Ala Asn Ile Gln Pro Ala Ala 275 280 285
Glu Leu Phe Ala Lys Ile Tyr Glu Asp Ala Gly Lys Pro Arg Ala Val 290
295 300 Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser Ile
Ser 305 310 315 320 Ser Pro Pro Pro Tyr Thr Ser Pro Asn Pro Asn Tyr
Asp Glu Lys His 325 330 335 Tyr Ile Glu Ala Phe Arg Pro Leu Leu Glu
Ala Arg Gly Phe Pro Ala 340 345 350 Gln Phe Ile Val Asp Gln Gly Arg
Ser Gly Lys Gln Pro Thr Gly Gln 355 360 365 Lys Glu Trp Gly His Trp
Cys Asn Ala Ile Gly Thr Gly Phe Gly Met 370 375 380 Arg Pro Thr Ala
Asn Thr Gly His Gln Tyr Val Asp Ala Phe Val Trp 385 390 395 400 Val
Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr Thr Ala Ala 405 410
415 Arg Tyr Asp Pro His Cys Gly Leu Glu Asp Ala Leu Lys Pro Ala Pro
420 425 430 Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu
Arg Asn 435 440 445 Ala Asn Pro Pro Phe 450 77440PRTPhanerochaete
chrysosporium 77Ala Ser Ser Glu Trp Gly Gln Cys Gly Gly Ile Gly Trp
Thr Gly Pro 1 5 10 15 Thr Thr Cys Val Ser Gly Thr Thr Cys Thr Val
Leu Asn Pro Tyr Tyr 20 25 30 Ser Gln Cys Leu Pro Gly Ser Ala Val
Thr Thr Thr Ser Val Ile Thr 35 40 45 Ser His Ser Ser Ser Val Ser
Ser Val Ser Ser His Ser Gly Ser Ser 50 55 60 Thr Ser Thr Ser Ser
Pro Thr Gly Pro Thr Gly Thr Asn Pro Pro Pro 65 70 75 80 Pro Pro Ser
Ala Asn Asn Pro Trp Thr Gly Phe Gln Ile Phe Leu Ser 85 90 95 Pro
Tyr Tyr Ala Asn Glu Val Ala Ala Ala Ala Lys Gln Ile Thr Asp 100 105
110 Pro Thr Leu Ser Ser Lys Ala Ala Ser Val Ala Asn Ile Pro Thr Phe
115 120 125 Thr Trp Leu Asp Ser Val Ala Lys Ile Pro Asp Leu Gly Thr
Tyr Leu 130 135 140 Ala Ser Ala Ser Ala Leu Gly Lys Ser Thr Gly Thr
Lys Gln Leu Val 145 150 155 160 Gln Ile Val Ile Tyr Asp Leu Pro Asp
Arg Asp Cys Ala Ala Lys Ala 165 170 175 Ser Asn Gly Glu Phe Ser Ile
Ala Asn Asn Gly Gln Ala Asn Tyr Glu 180 185 190 Asn Tyr Ile Asp Gln
Ile Val Ala Gln Ile Gln Gln Phe Pro Asp Val 195 200 205 Arg Val Val
Ala Val Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr 210 215 220 Asn
Leu Asn Val Gln Lys Cys Ala Asn Ala Lys Thr Thr Tyr Leu Ala 225 230
235 240 Cys Val Asn Tyr Ala Leu Thr Asn Leu Ala Lys Val Gly Val Tyr
Met 245 250 255 Tyr Met Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro
Ala Asn Leu 260 265 270 Ser Pro Ala Ala Gln Leu Phe Thr Gln Val Trp
Gln Asn Ala Gly Lys 275 280 285 Ser Pro Phe Ile Lys Gly Leu Ala Thr
Asn Val Ala Asn Tyr Asn Ala 290 295 300 Leu Gln Ala Ala Ser Pro Asp
Pro Ile Thr Gln Gly Asn Pro Asn Tyr 305 310 315 320 Asp Glu Ile His
Tyr Ile Asn Ala Leu Ala Pro Leu Leu Gln Gln Ala 325 330 335 Gly Trp
Asp Ala Thr Phe Ile Val Asp Gln Gly Arg Ser Gly Val Gln 340 345 350
Asn Ile Arg Gln Gln Trp Gly Asp Trp Cys Asn Ile Lys Gly Ala Gly 355
360 365 Phe Gly Thr Arg Pro Thr Thr Asn Thr Gly Ser Gln Phe Ile Asp
Ser 370 375 380 Ile Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr
Ser Asn Ser 385 390 395 400 Ser Ser Pro Arg Tyr Asp Pro Thr Cys Ser
Leu Pro Asp Ala Ala Gln 405 410 415 Pro Ala Pro Glu Ala Gly Thr Trp
Phe Gln Ala Tyr Phe Gln Thr Leu 420 425 430 Val Ser Ala Ala Asn Pro
Pro Leu 435 440 78459PRTTrichoderma reesei 78Asp Tyr Lys Asp Asp
Asp Asp Lys Glu Phe Leu Glu Ala Ser Cys Ser 1 5 10 15 Ser Val Trp
Gly Gln Cys Gly Gly Gln Asn Trp Ser Gly Pro Thr Cys 20 25 30 Cys
Ala Ser Gly Ser Thr Cys Val Tyr Ser Asn Asp Tyr Tyr Ser Gln 35 40
45 Cys Leu Pro Gly Ala Ala Ser Ser Ser Ser Ser Thr Arg Ala Ala Ser
50 55 60 Thr Thr Ser Arg Val Ser Pro Thr Thr Ser Arg Ser Ser Ser
Ala Thr 65 70 75 80 Pro Pro Pro Gly Ser Thr Thr Thr Arg Val Pro Pro
Val Gly Ser Gly 85 90 95 Thr Ala Thr Tyr Ser Gly Asn Pro Phe Val
Gly Val Thr Pro Trp Ala 100 105 110 Asn Ala Tyr Tyr Ala Ser Glu Val
Ser Ser Leu Ala Ile Pro Ser Leu 115 120 125 Thr Gly Ala Met Ala Thr
Ala Ala Ala Ala Val Ala Lys Val Pro Ser 130 135 140 Phe Met Trp Leu
Asp Thr Leu Asp Lys Thr Pro Leu Met Glu Gln Thr 145 150 155 160 Leu
Ala Asp Ile Arg Thr Ala Asn Lys Asn Gly Gly Asn Tyr Ala Gly 165 170
175 Gln Phe Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Leu Ala
180 185 190 Ser Asn Gly Glu Tyr Ser Ile Ala Asp Gly Gly Val Ala Lys
Tyr Lys 195 200 205 Asn Tyr Ile Asp Thr Ile Arg Gln Ile Val Val Glu
Tyr Ser Asp Ile 210 215 220 Arg Thr Leu Leu Val Ile Glu Pro Asp Ser
Leu Ala Asn Leu Val Thr 225 230 235 240 Asn Leu Gly Thr Pro Lys Cys
Ala Asn Ala Gln Ser Ala Tyr Leu Glu 245 250 255 Cys Ile Asn Tyr Ala
Val Thr Gln Leu Asn Leu Pro Asn Val Ala Met 260 265 270 Tyr Leu Asp
Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Gln 275 280 285 Asp
Pro Ala Ala Gln Leu Phe Ala Asn Val Tyr Lys Asn Ala Ser Ser 290 295
300 Pro Arg Ala Leu Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly
305 310 315 320 Trp Asn Ile Thr Ser Pro Pro Ser Tyr Thr Gln Gly Asn
Ala Val Tyr 325 330 335 Asn Glu Lys Leu Tyr Ile His Ala Ile Gly Pro
Leu Leu Ala Asn His 340 345 350 Gly Trp Ser Asn Ala Phe Phe Ile Thr
Asp Gln Gly Arg Ser Gly Lys 355 360 365 Gln Pro Thr Gly Gln Gln Gln
Trp Gly Asp Trp Cys Asn Val Ile Gly 370 375 380 Thr Gly Phe Gly Ile
Arg Pro Ser Ala Asn Thr Gly Asp Ser Leu Leu 385 390 395 400 Asp Ser
Phe Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser 405 410 415
Asp Ser Ser Ala Pro Arg Phe Asp Ser His Cys Ala Leu Pro Asp Ala 420
425 430 Leu Gln Pro Ala Pro Gln Ala Gly Ala Trp Phe Gln Ala Tyr Phe
Val 435 440 445 Gln Leu Leu Thr Asn Ala Asn Pro Ser Phe Leu 450 455
79484PRTPodospora anserina 79Met Ala Lys Arg Leu Leu Leu Thr Ala
Ala Leu Ala Ala Thr Thr Leu 1 5 10 15 Ala Ala Pro Val Ile Glu Glu
Arg Gln Asn Cys Gly Ser Val Trp Ser 20 25 30 Gln Cys Gly Gly Gln
Gly Trp Thr Gly Ala Thr Cys Cys Ala Ser Gly 35 40 45 Ser Thr Cys
Val Ala Gln Asn Gln Trp Tyr Ser Gln Cys Leu Pro Gly 50 55 60 Ser
Gln Val Thr Thr Thr Ala Gln Ala Pro Ser Ser Thr Arg Thr Thr 65 70
75 80 Thr Ser Ser Ser Ser Arg Pro Thr Ser Ser Ser Ile Ser Thr Ser
Ala 85 90 95 Val Asn Val Pro Thr Thr Thr Thr Ser Ala Gly Ala Ser
Val Thr Val 100 105 110 Pro Pro Gly Gly Gly Ala Ser Ser Thr Ala Ser
Tyr Ser Gly Asn Pro 115 120 125 Phe Leu Gly Val Gln Gln Trp Ala Asn
Ser Tyr Tyr Ser Ser Glu Val 130 135 140 His Thr Leu Ala Ile Pro Ser
Leu Thr Gly Pro Met Ala Thr Lys Ala 145 150 155 160 Ala Ala Val Ala
Lys Val Pro Ser Phe Gln Trp Met Asp Arg Asn Val 165 170 175 Thr Val
Asp Thr Leu Phe Ser Gly Thr Leu Ala Asp Ile Arg Ala Ala 180 185 190
Asn Arg Ala Gly Ala Asn Pro Pro Tyr Ala Gly Ile Phe Val Val Tyr 195
200 205 Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn Gly Glu
Trp 210 215 220 Ala Ile Ala Asp Gly Gly Ala Ala Lys Tyr Lys Ala Tyr
Ile Asp Arg 225 230 235 240 Ile Arg His His Leu Val Gln Tyr Ser Asp
Ile Arg Thr Ile Leu Val 245 250 255 Ile Glu Pro Asp Ser Leu Ala Asn
Met Val Thr Asn Met Asn Val Pro 260 265 270 Lys Cys Gln Gly Ala Ala
Asn Thr Tyr Lys Glu Leu Thr Val Tyr Ala 275 280 285 Leu Lys Gln Leu
Asn Leu Pro Asn Val Ala Met Tyr Leu Asp Ala Gly 290 295 300 His Ala
Gly Trp Leu Gly Trp Pro Ala Asn Ile Gly Pro Ala Ala Glu 305 310 315
320 Leu Phe Ala Gly Ile Tyr Lys Asp Ala Gly Arg Pro Thr Ser Leu Arg
325 330 335 Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Gly Trp Ser Leu
Ser Ser 340 345 350 Ala Pro Ser Tyr Thr Thr Pro Asn Pro Asn Phe Asp
Glu Lys Arg Phe 355 360 365 Val Gln Ala Phe Ser Pro Leu Leu Thr Ala
Ala Gly Phe Pro Ala His 370 375 380 Phe Ile Thr Asp Thr Gly Arg Ser
Gly Lys Gln Pro Thr Gly Gln Leu 385 390 395 400 Glu Trp Gly His Trp
Cys Asn Ala Ile Gly Thr Gly Phe Gly Pro Arg 405 410 415 Pro Thr Thr
Asp Thr Gly Leu Asp Ile Glu Asp Ala Phe Val Trp Ile 420 425 430 Lys
Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr Thr Ala Ala Arg 435 440
445 Tyr Asp His His Cys Gly Phe Ala Asp Ala Leu Lys Pro Ala Pro Glu
450 455 460 Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu Thr
Asn Ala 465 470 475 480 Asn Pro Pro Phe 80485PRTChaetomium Globosum
80Met Ala Ala Lys Leu Phe Leu Ala Ala Ala Leu Ala Ala Thr Ala Leu 1
5 10 15 Ala Ala Pro Val Val Glu Glu Arg Gln Asn Cys Ala Thr Leu Trp
Gly 20 25 30 Gln Cys Gly Gly Asn Gly Trp Asn Gly Ala Thr Cys Cys
Ala Ser Gly 35 40 45 Ser Thr Cys Thr Lys Gln Asn Asp Trp Tyr Ser
Gln Cys Leu Pro Gly 50 55 60 Gly Ala Val Thr Thr Pro Gly Thr Thr
Thr Lys Pro Thr Ser Thr Ser 65 70 75 80 Thr Ser Thr Ser Thr Ser Ser
Arg Ser Thr Ser Thr Ser Gln Gly Gly 85 90 95 Gly Val Ser Ser Ser
Thr Ser Ser Pro Pro Val Val Thr Asn Pro Pro 100 105 110 Thr Ser Ile
Pro Gly Gly Ala Ser Ser Thr Ala Ser Tyr Thr Gly Asn 115 120 125 Pro
Phe Ser Gly Val Gln Met Trp Ala Asn Asp Tyr Tyr Arg Ser Glu 130 135
140 Val His Thr Leu Ala Met Pro Ser Leu Thr Gly Ala Met Ala Thr Lys
145 150 155 160 Ala Ala Lys Val Ala Glu Val Pro Ser Tyr Gln Trp Met
Asp Arg Asn 165 170 175 Val Thr Val Asp Thr Leu Phe Ser Gly Thr Leu
Ala Gln Ile Arg Ala 180 185 190 Ala Asn Gln Ala Gly Ala Ser Pro Pro
Tyr Ala Gly Ile Phe Val Val 195 200 205 Tyr Asp Leu Pro Asp Arg Asp
Cys Ala Ala Ala Ala Ser Asn Gly Glu 210 215 220 Trp Ser Ile Ala Asn
Gly Gly Ala Ala Asn Tyr Lys Ala Tyr Ile Lys 225 230 235 240 Arg Ile
Arg Glu Leu Ile Ile Gln Tyr Ser Asp Ile Arg Met Leu Leu 245 250 255
Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn Met Gly Val 260
265 270 Ala Lys Cys Ala Gly Ala Ala Ser Thr Tyr Lys Glu Leu Thr Ile
His 275 280 285 Ala Leu Lys Glu Leu Asn Leu Pro Asn Val Ala Met Tyr
Leu Asp Ala 290 295 300 Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn
Ile Gln Pro Ala Ala 305 310 315 320 Asp Leu Phe Ala Thr Leu Tyr Lys
Asp Ala Gly Arg Pro Ala Ala Val
325 330 335 Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Ser
Val Ser 340 345 350 Ser Ala Pro Ala Tyr Thr Ser Pro Asn Pro Asn Tyr
Asp Glu Lys His 355 360 365 Tyr Val Glu Ala Phe Ser Pro Leu Leu Thr
Ala Ala Gly Phe Pro Ala 370 375 380 His Phe Ile Thr Asp Thr Gly Arg
Ser Gly Lys Gln Pro Thr Gly Gln 385 390 395 400 Leu Glu Trp Gly His
Trp Cys Asn Ala Val Gly Thr Gly Phe Gly Gln 405 410 415 Arg Pro Ser
Ala Asn Thr Gly His Asp Leu Leu Asp Ala Phe Val Trp 420 425 430 Ile
Lys Pro Gly Gly Glu Cys Asp Gly Thr Ser Asp Thr Thr Ala Ala 435 440
445 Arg Tyr Asp His Asn Cys Gly Leu Ala Asp Ala Leu Lys Pro Ala Pro
450 455 460 Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu Gln Leu Leu
Thr Asn 465 470 475 480 Ala Asn Pro Pro Phe 485 81487PRTMagnaporthe
grisea 81Met Ala Ser Lys Leu Phe Leu Ala Ala Ala Leu Leu Gln Gly
Ala Leu 1 5 10 15 Ser Ser Pro Leu Ala Val Glu Glu Arg Gln Ala Cys
Ala Ala Gln Trp 20 25 30 Gly Gln Cys Gly Gly Gln Asp Tyr Thr Gly
Pro Thr Cys Cys Gln Ser 35 40 45 Gly Ser Thr Cys Val Val Ser Asn
Gln Trp Tyr Ser Gln Cys Leu Pro 50 55 60 Gly Ser Ser Asn Pro Thr
Thr Thr Ser Arg Thr Ser Thr Ser Ser Ser 65 70 75 80 Ser Ser Thr Ser
Arg Thr Ser Ser Ser Thr Ser Arg Pro Pro Ser Ser 85 90 95 Val Pro
Thr Thr Pro Thr Ser Val Pro Pro Thr Ile Thr Thr Thr Pro 100 105 110
Thr Thr Thr Pro Thr Gly Gly Ser Gly Pro Gly Thr Thr Ala Ser Phe 115
120 125 Thr Gly Asn Pro Phe Ala Gly Val Asn Leu Phe Pro Asn Lys Phe
Tyr 130 135 140 Ser Ser Glu Val His Thr Leu Ala Ile Pro Ser Leu Thr
Gly Ser Leu 145 150 155 160 Val Ala Lys Ala Ser Ala Val Ala Gln Val
Pro Ser Phe Gln Trp Leu 165 170 175 Asp Ile Ala Ala Lys Val Glu Thr
Leu Met Pro Gly Ala Leu Ala Asp 180 185 190 Val Arg Ala Ala Asn Ala
Ala Gly Gly Asn Tyr Ala Ala Gln Leu Val 195 200 205 Val Tyr Asp Leu
Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn Gly 210 215 220 Glu Phe
Ser Ile Ala Asp Gly Gly Val Val Lys Tyr Lys Ala Tyr Ile 225 230 235
240 Asp Ala Ile Arg Lys Gln Leu Leu Ala Tyr Ser Asp Val Arg Thr Ile
245 250 255 Leu Val Ile Glu Pro Asp Ser Leu Ala Asn Met Val Thr Asn
Met Gly 260 265 270 Val Pro Lys Cys Ala Gly Ala Lys Asp Ala Tyr Leu
Glu Cys Thr Ile 275 280 285 Tyr Ala Val Lys Gln Leu Asn Leu Pro His
Val Ala Met Tyr Leu Asp 290 295 300 Gly Gly His Ala Gly Trp Leu Gly
Trp Pro Ala Asn Leu Gln Pro Ala 305 310 315 320 Ala Asp Leu Phe Gly
Lys Leu Tyr Ala Asp Ala Gly Lys Pro Ser Gln 325 330 335 Leu Arg Gly
Met Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Asp Leu 340 345 350 Thr
Thr Ala Pro Ser Tyr Thr Thr Pro Asn Pro Asn Phe Asp Glu Lys 355 360
365 Lys Tyr Ile Ser Ala Phe Ala Pro Leu Leu Ala Ala Lys Gly Trp Ser
370 375 380 Ala His Phe Ile Ile Asp Gln Gly Arg Ser Gly Lys Gln Pro
Thr Gly 385 390 395 400 Gln Lys Glu Trp Gly His Trp Cys Asn Gln Gln
Gly Val Gly Phe Gly 405 410 415 Arg Arg Pro Ser Ala Asn Thr Gly Ser
Glu Leu Ala Asp Ala Phe Val 420 425 430 Trp Ile Lys Pro Gly Gly Glu
Cys Asp Gly Val Ser Asp Pro Thr Ala 435 440 445 Pro Arg Phe Asp His
Phe Cys Gly Thr Asp Tyr Gly Ala Met Ser Asp 450 455 460 Ala Pro Gln
Ala Gly Gln Trp Phe Gln Lys Tyr Phe Glu Met Leu Leu 465 470 475 480
Thr Asn Ala Asn Pro Pro Leu 485 82421PRTSclerotinia sclerotiorum
82Met Gly Leu Lys Asn Val Leu Leu Ala Ala Ala Ala Val Ala Pro Thr 1
5 10 15 Val Tyr Ala Gln Gly Ala Gly Tyr Ser Gln Cys Gly Gly Gln Gly
Trp 20 25 30 Ser Gly Ala Thr Thr Cys Val Ser Gly Phe Thr Cys Thr
Tyr Thr Asn 35 40 45 Glu Tyr Tyr Ser Gln Cys Leu Pro Gly Ser Gly
Gly Gly Ala Ser Ser 50 55 60 Ser Arg Pro Thr Thr Thr Ala Pro Thr
Thr Ile Val Thr Ser Thr Lys 65 70 75 80 Ala Ser Thr Thr Thr Gly Ser
Ser Ala Thr Thr Thr Ala Ala Pro Ala 85 90 95 Ala Gly Asn Pro Phe
Val Gly Lys Ala Leu Tyr Val Asn Pro Tyr Tyr 100 105 110 Ala Ser Glu
Ile Ser Ala Ser Ala Ile Pro Ser Leu Thr Gly Ala Met 115 120 125 Ala
Thr Lys Ala Ala Ala Val Ala Lys Val Pro Thr Phe Phe Trp Leu 130 135
140 Asp Thr Ala Asp Lys Val Pro Thr Met Gly Thr Tyr Leu Ser Asn Ile
145 150 155 160 Arg Ala Leu Asn Lys Ala Gly Ala Asn Pro Pro Val Ala
Gly Thr Phe 165 170 175 Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala
Ala Ala Ala Ser Asn 180 185 190 Gly Glu Tyr Ser Ile Ala Asn Asn Gly
Val Ala Asn Tyr Lys Ala Tyr 195 200 205 Ile Asp Ser Ile Val Thr Ile
Leu Lys Asn Tyr Ser Asp Thr Ser Val 210 215 220 Ile Leu Ile Ile Val
Asp Leu Pro Asn Val Ser Met Tyr Leu Asp Ala 225 230 235 240 Gly His
Ala Gly Trp Leu Gly Trp Ser Ala Asn Ile Gly Pro Ala Ala 245 250 255
Gln Leu Phe Gly Gln Val Tyr Lys Ala Ala Gly Ser Pro Ser Gln Val 260
265 270 Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr Asn Ala Trp Thr Ser
Ser 275 280 285 Ser Cys Pro Ser Tyr Thr Ser Gly Asp Ser Asn Cys Asn
Glu Lys Leu 290 295 300 Tyr Ile Asn Ala Leu Ala Pro Leu Leu Thr Ala
Gln Gly Phe Pro Ala 305 310 315 320 His Phe Ile Met Asp Thr Gly Arg
Asn Gly Val Gln Pro Thr Ala Gln 325 330 335 Gln Ala Trp Gly Asp Trp
Cys Asn Val Ile Gly Thr Gly Phe Gly Val 340 345 350 Arg Pro Thr Thr
Asn Thr Gly Asp Ala Leu Glu Asp Ala Phe Val Trp 355 360 365 Val Lys
Pro Gly Gly Glu Ala Asp Gly Thr Ser Asn Thr Thr Ala Ala 370 375 380
Arg Tyr Asp Phe His Cys Gly Leu Ser Asp Ala Leu Gln Pro Ala Pro 385
390 395 400 Glu Ala Gly Thr Trp Phe Gln Ala Tyr Phe Ala Gln Leu Leu
Thr Asn 405 410 415 Ala Asn Pro Ser Phe 420 83449PRTPhaeosphaeria
nodorum 83Met Lys Ala Val Ser Phe Leu Ala Val Ala Ala Leu Ala Pro
Ala Ile 1 5 10 15 Lys Ala Gln Ala Ser Leu Tyr Gln Gln Cys Gly Gly
Thr Gly Phe Ser 20 25 30 Gly Ser Thr Thr Cys Val Ser Gly Ala Tyr
Cys Ser Lys Val Asn Asp 35 40 45 Ser Ala Thr Ser Ala Ala Pro Ala
Pro Thr Thr Phe Lys Thr Ser Lys 50 55 60 Thr Val Gly Ser Pro Ala
Thr Gly Ser Ser Thr Thr Gly Ser Ser Ala 65 70 75 80 Thr Gly Thr Ala
Ser Pro Gly Asp Gly Ser Asn Pro Leu Lys Gly Lys 85 90 95 Asn Phe
Tyr Ala Asn Ser Tyr Tyr Ala Ser Glu Ile Asn Asn Leu Ala 100 105 110
Ala Pro Ser Leu Val Ala Ala Gly Asn Ala Ala Leu Ala Ala Lys Ala 115
120 125 Ser Asn Val Ala Lys Val Gly Thr Phe Tyr Trp Leu Asp Val Arg
Ala 130 135 140 Lys Val Pro Ile Ile Ser Thr Phe Ala Lys Asp Val Gln
Lys Arg Asn 145 150 155 160 Ala Ala Gly Ala Asn Glu Val Leu Pro Leu
Val Val Tyr Asp Leu Pro 165 170 175 Glu Arg Asp Cys Ala Ala Leu Ala
Ser Asn Gly Glu Leu Ser Leu Ala 180 185 190 Asn Asn Gly Thr Ala Leu
Tyr Gln Glu Tyr Ile Asp Met Ile Ala Ala 195 200 205 Gln Ile Lys Gln
Phe Pro Asp Val Thr Phe Leu Leu Val Val Glu Pro 210 215 220 Asp Ser
Leu Ala Asn Leu Val Thr Asn Leu Asn Val Ala Lys Cys Ala 225 230 235
240 Asn Ala Ala Thr Ala Tyr Lys Thr Leu Thr Ala Tyr Ala Ile Lys Thr
245 250 255 Leu Asn Leu Lys Asn Val Ile Met Tyr Leu Asp Ala Gly His
Ala Gly 260 265 270 Trp Leu Gly Trp Thr Ala Asn Ile Glu Pro Ala Ala
Glu Leu Phe Gly 275 280 285 Ala Leu Tyr Lys Ser Ala Gly Ser Pro Ala
Ala Val Arg Gly Leu Val 290 295 300 Thr Asn Val Ala Asn Tyr Asn Ala
Trp Ser Ile Ala Thr Cys Pro Ser 305 310 315 320 Tyr Thr Gln Gly Asn
Thr Asn Cys Asp Glu Lys Arg Tyr Val Asn Ala 325 330 335 Leu Ala Pro
Leu Leu Val Lys Asn Gly Phe Pro Ala His Phe Leu Thr 340 345 350 Asp
Thr Gly Arg Asn Gly Val Gln Pro Thr Lys Gln Gln Ala Trp Gly 355 360
365 Asp Trp Cys Asn Val Ile Gly Thr Gly Phe Gly Ile Arg Pro Ser Ser
370 375 380 Thr Thr Asp Asp Pro Leu Leu Asp Ala Tyr Val Trp Val Lys
Pro Gly 385 390 395 400 Gly Glu Gly Asp Gly Thr Ser Asp Thr Ser Ala
Val Arg Tyr Asp Ala 405 410 415 His Cys Gly Tyr Ala Asp Ala Leu Lys
Pro Ala Pro Glu Ala Gly Ser 420 425 430 Trp Phe Gln Ala Tyr Phe Val
Gln Leu Leu Ser Asn Ala Ser Pro Ala 435 440 445 Phe
84438PRTAgaricus bisporus 84Met Phe Lys Phe Ala Ala Leu Leu Ala Leu
Ala Ser Leu Val Pro Gly 1 5 10 15 Phe Val Gln Ala Gln Ser Pro Val
Trp Gly Gln Cys Gly Gly Asn Gly 20 25 30 Trp Thr Gly Pro Thr Thr
Cys Ala Ser Gly Ser Thr Cys Val Lys Gln 35 40 45 Asn Asp Phe Tyr
Ser Gln Cys Leu Pro Asn Asn Gln Ala Pro Pro Ser 50 55 60 Thr Thr
Thr Gln Pro Gly Thr Thr Pro Pro Ala Thr Thr Thr Ser Gly 65 70 75 80
Gly Thr Gly Pro Thr Ser Gly Ala Gly Asn Pro Tyr Thr Gly Lys Thr 85
90 95 Val Trp Leu Ser Pro Phe Tyr Ala Asp Glu Val Ala Gln Ala Ala
Ala 100 105 110 Asp Ile Ser Asn Pro Ser Leu Ala Thr Lys Ala Ala Ser
Val Ala Lys 115 120 125 Ile Pro Thr Phe Thr Trp Phe Asp Thr Val Ala
Lys Val Pro Asp Leu 130 135 140 Gly Gly Tyr Leu Ala Asp Ala Gln Ser
Lys Asn Gln Leu Val Gln Ile 145 150 155 160 Val Val Tyr Asp Leu Pro
Asp Arg Asp Cys Ala Ala Leu Ala Ser Asn 165 170 175 Gly Glu Phe Ser
Leu Ala Asn Asp Gly Leu Asn Lys Tyr Lys Asn Tyr 180 185 190 Val Asp
Gln Ile Ala Ala Gln Ile Lys Gln Phe Pro Asp Val Ser Val 195 200 205
Val Ala Val Ile Glu Pro Asp Ser Leu Ala Asn Leu Val Thr Asn Leu 210
215 220 Asn Val Gln Lys Cys Ala Asn Ala Gln Ser Ala Tyr Lys Glu Gly
Val 225 230 235 240 Ile Tyr Ala Ile Gln Lys Leu Asp Ala Val Gly Val
Thr Met Tyr Ile 245 250 255 Asp Ala Gly His Ala Gly Trp Leu Gly Trp
Pro Ala Asn Leu Ser Pro 260 265 270 Ala Ala Gln Leu Phe Ala Gln Ile
Tyr Arg Asp Ala Gly Ser Pro Arg 275 280 285 Asn Leu Arg Gly Ile Ala
Thr Asn Val Ala Asn Phe Asn Ala Leu Arg 290 295 300 Ala Ser Ser Pro
Asp Pro Ile Thr Gln Gly Asn Ser Asn Tyr Asp Glu 305 310 315 320 Ile
His Tyr Ile Glu Ala Leu Ala Pro Met Leu Ser Asn Ala Gly Phe 325 330
335 Pro Ala His Phe Ile Val Asp Gln Gly Arg Ser Gly Val Gln Asn Ile
340 345 350 Arg Asp Gln Trp Gly Asp Trp Cys Asn Val Lys Gly Ala Gly
Phe Gly 355 360 365 Gln Arg Pro Thr Thr Asn Thr Gly Ser Ser Leu Ile
Asp Ala Ile Val 370 375 380 Trp Val Lys Pro Gly Gly Glu Cys Asp Gly
Thr Ser Asp Ser Ser Ser 385 390 395 400 Pro Arg Phe Asp Ser His Cys
Ser Leu Ser Asp Ala His Gln Pro Ala 405 410 415 Pro Glu Ala Gly Thr
Trp Phe Gln Ala Tyr Phe Glu Thr Leu Val Ala 420 425 430 Asn Ala Asn
Pro Ala Leu 435 85443PRTLentinula edodes 85Met Lys Ile Thr Ser Thr
Gly Leu Leu Ala Leu Ser Ser Leu Leu Pro 1 5 10 15 Phe Ala Leu Gly
Gln Ser Gln Leu Tyr Ala Gln Cys Gly Gly Ile Gly 20 25 30 Trp Ser
Gly Ala Thr Thr Cys Val Ser Gly Ala Thr Cys Thr Val Val 35 40 45
Asn Ala Tyr Tyr Ser Gln Cys Leu Pro Gly Ser Ala Ser Ala Pro Pro 50
55 60 Thr Ser Thr Ser Ser Ile Gly Thr Gly Thr Thr Thr Ser Ser Ala
Pro 65 70 75 80 Gly Ser Thr Gly Thr Thr Thr Pro Ala Ala Gly Asn Pro
Phe Thr Glu 85 90 95 Gln Ile Tyr Leu Ser Pro Tyr Tyr Ala Asn Glu
Ile Ala Ala Ala Val 100 105 110 Thr Gln Ile Ser Asp Pro Thr Thr Ala
Ala Ala Ala Ala Lys Val Ala 115 120 125 Asn Ile Pro Thr Phe Ile Trp
Leu Asp Gln Val Ala Lys Val Pro Asp 130 135 140 Leu Gly Thr Tyr Leu
Ala Asp Ala Ser Ala Lys Gln Lys Ser Glu Gly 145 150 155 160 Lys Asn
Tyr Leu Val Gln Ile Val Val Tyr Asp Leu Pro Asp Arg Asp 165 170 175
Cys Ala Ala Leu Ala Ser Asn Gly Glu Phe Thr Ile Ala Asp Asn Gly 180
185 190 Glu Ala Asn Tyr His Asp Tyr Ile Asp Gln Ile Val Ala Gln Ile
Lys 195 200 205 Gln Tyr Pro Asp Val His Val Val Ala Val Ile Glu Pro
Asp Ser Leu 210 215 220 Ala Asn Leu Val Thr Asn Leu Ser Val Ala Lys
Cys Ala Asn Ala Gln 225 230 235 240 Thr Thr Tyr Leu Glu Cys Val Thr
Tyr Ala Met Gln Gln Leu Ser Ala 245 250 255 Val Gly Val Thr Met Tyr
Leu Asp Ala Gly His Ala Gly Trp Leu Gly 260 265 270 Trp Pro Ala Asn
Leu Ser Pro Ala Ala Gln Leu Phe Thr Ser Leu Tyr 275 280 285 Ser Asn
Ala Gly Ser Pro Ser Gly Val Arg Gly Leu Ala Thr Asn Val 290 295 300
Ala Asn Tyr Asn Ala Leu Val Ala Thr Thr Pro Asp Pro Ile Thr Gln 305
310 315 320 Gly Asp Pro Asn Tyr Asp Glu Met Leu Tyr Ile Glu Ala Leu
Ala Pro 325 330 335 Leu Leu
Gly Ser Phe Pro Ala His Phe Ile Val Asp Gln Gly Arg Ser 340 345 350
Gly Val Gln Asp Ile Arg Gln Gln Trp Gly Asp Trp Cys Asn Val Leu 355
360 365 Gly Ala Gly Phe Gly Thr Gln Pro Thr Thr Asn Thr Gly Ser Ser
Leu 370 375 380 Ile Asp Ser Ile Val Trp Val Lys Pro Gly Gly Glu Cys
Asp Gly Thr 385 390 395 400 Ser Asn Thr Ser Ser Pro Arg Tyr Asp Ala
His Cys Gly Leu Pro Asp 405 410 415 Ala Thr Pro Asn Ala Pro Glu Ala
Gly Thr Trp Phe Gln Ala Tyr Phe 420 425 430 Glu Thr Leu Val Glu Lys
Ala Asn Pro Pro Leu 435 440 86460PRTConiophora puteana 86Met Phe
Lys Phe Ala Ala Leu Ser Ala Phe Val Ala Leu Val Pro Leu 1 5 10 15
Leu Val Asn Ala Gln Val Ala Ala Tyr Gly Gln Cys Gly Gly Gln Asp 20
25 30 Trp Thr Gly Ala Thr Ala Cys Ala Ser Gly Thr Ala Cys Thr Lys
Val 35 40 45 Asn Asp Tyr Tyr Tyr Gln Cys Leu Pro Gly Ser Ser Gly
Ser Ser Val 50 55 60 Ser Gly Gly Ser Gly Ser Gly Ser Thr Ser Ala
Pro Ser Pro Thr Ser 65 70 75 80 Thr Val Pro Thr Ser Thr Ser Ser Ala
Ser Thr Ala Pro Ser Ser Thr 85 90 95 Ser Thr Ser Ser Ala Ala Ser
Ser Asp Asn Pro Tyr Thr Gly Tyr Gln 100 105 110 Ile Phe Leu Asn Pro
Glu Tyr Ala Ser Glu Val Gln Ala Ala Ile Pro 115 120 125 Ser Ile Thr
Asp Ser Ala Val Ala Ala Lys Ala Leu Lys Val Ala Glu 130 135 140 Val
Pro Val Phe Phe Trp Leu Asp Gln Val Ala Lys Val Pro Asp Leu 145 150
155 160 Glu Thr Tyr Leu Ala Ala Ala Asp Lys Gln Gly Lys Ser Ser Gly
Gln 165 170 175 Lys Gln Leu Leu Gln Ile Val Val Tyr Asp Leu Pro Asp
Arg Asp Cys 180 185 190 Ala Ala Asn Ala Ser Asn Gly Glu Phe Ser Ile
Ser Asp Asp Gly Gln 195 200 205 Ala Lys Tyr Glu Asn Tyr Ile Asp Gln
Ile Val Ala Ile Val Lys Lys 210 215 220 Tyr Pro Asp Val Arg Val Val
Ala Val Val Glu Pro Asp Ser Met Gly 225 230 235 240 Asn Leu Val Thr
Asn Met Asp Leu Pro Lys Cys Ser Ala Ala Ala Pro 245 250 255 Thr Tyr
Lys Thr Cys Ile Asn Tyr Ala Ile Ala Gln Leu Ser Ser Ala 260 265 270
Gly Val Tyr Met Tyr Val Asp Ala Gly His Ala Gly Trp Leu Gly Trp 275
280 285 Pro Asn Asn Leu Ala Pro Ala Ala Gln Leu Phe Gly Glu Leu Tyr
Glu 290 295 300 Thr Ser Gly Lys Ser Ala Tyr Phe Arg Gly Leu Ala Thr
Asn Val Ala 305 310 315 320 Asn Tyr Asn Ala Leu Asn Thr Ser Ser Pro
Asp Pro Cys Thr Gln Asn 325 330 335 Ala Pro Asn Tyr Asp Glu Met Leu
Tyr Ile Asn Ala Leu Ser Pro Leu 340 345 350 Leu Gln Gln Gln Gly Phe
Ser Ala Gln Phe Ile Val Asp Gln Gly Arg 355 360 365 Ser Gly Val Gln
Asn Ile Arg Asn Ala Trp Gly Asp Trp Cys Asn Ile 370 375 380 Lys Gly
Ala Gly Phe Gly Ile Arg Pro Thr Thr Asp Thr Gly Ser Pro 385 390 395
400 Leu Ile Asp Ser Ile Val Trp Val Lys Pro Gly Gly Glu Cys Asp Gly
405 410 415 Thr Ser Asn Ser Ser Ala Pro Arg Tyr Asp Ser Thr Cys Ser
Leu Ser 420 425 430 Asp Ser Leu Gln Pro Ala Pro Glu Ala Gly Thr Trp
Phe Gln Gln Tyr 435 440 445 Phe Glu Ala Leu Val Thr Asn Ala Val Pro
Ser Leu 450 455 460 87442PRTVolvariella volvacea 87Met Ser Arg Phe
Ser Ala Leu Thr Ala Leu Leu Leu Ser Leu Pro Leu 1 5 10 15 Leu Ala
Ile Ala Gln Ser Pro Leu Tyr Gly Gln Cys Gly Gly Asn Gly 20 25 30
Trp Thr Gly Pro Lys Thr Cys Val Ser Gly Ala Thr Cys Thr Val Ile 35
40 45 Asn Asp Trp Tyr Trp Gln Cys Leu Pro Gly Asn Gly Pro Thr Ser
Ser 50 55 60 Ser Pro Thr Ser Thr Pro Thr Thr Thr Thr Thr Thr Gly
Gly Pro Gln 65 70 75 80 Pro Thr Val Pro Ala Ala Gly Asn Pro Tyr Thr
Gly Tyr Glu Ile Tyr 85 90 95 Leu Ser Pro Tyr Tyr Ala Ala Glu Ala
Gln Ala Ala Ala Ala Gln Ile 100 105 110 Ser Asp Ala Thr Gln Lys Ala
Lys Ala Leu Lys Val Ala Gln Ile Pro 115 120 125 Thr Phe Thr Trp Phe
Asp Val Ile Ala Lys Thr Ser Thr Leu Gly Asp 130 135 140 Tyr Leu Ala
Glu Ala Ser Ala Leu Gly Lys Ser Ser Gly Lys Lys Tyr 145 150 155 160
Leu Val Gln Ile Val Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala 165
170 175 Leu Ala Ser Asn Gly Glu Phe Ser Ile Ala Asn Asn Gly Leu Asn
Asn 180 185 190 Tyr Lys Gly Tyr Ile Asp Gln Leu Val Ala Gln Ile Lys
Lys Tyr Pro 195 200 205 Asp Val Arg Val Val Ala Val Ile Glu Pro Asp
Ser Leu Ala Asn Leu 210 215 220 Val Thr Asn Leu Asn Val Ser Lys Cys
Ala Asn Ala Gln Thr Ala Tyr 225 230 235 240 Lys Ala Gly Val Thr Tyr
Ala Leu Gln Gln Leu Asn Ser Val Gly Val 245 250 255 Tyr Met Tyr Leu
Asp Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala 260 265 270 Asn Leu
Asn Pro Ala Ala Gln Leu Phe Ser Gln Leu Tyr Arg Asp Ala 275 280 285
Gly Ser Pro Gln Tyr Val Arg Gly Leu Ala Thr Asn Val Ala Asn Tyr 290
295 300 Asn Ala Leu Ser Ala Ser Ser Pro Asp Pro Val Thr Gln Gly Asn
Pro 305 310 315 320 Asn Tyr Asp Glu Leu His Tyr Ile Asn Ala Leu Ala
Pro Ala Leu Gln 325 330 335 Ser Gly Gly Phe Pro Ala His Phe Ile Val
Asp Gln Gly Arg Ser Gly 340 345 350 Val Gln Asn Ile Arg Gln Gln Trp
Gly Asp Trp Cys Asn Val Lys Gly 355 360 365 Ala Gly Phe Gly Gln Arg
Pro Thr Leu Ser Thr Gly Ser Ser Leu Ile 370 375 380 Asp Ala Ile Val
Trp Ile Lys Pro Gly Gly Glu Cys Asp Gly Thr Thr 385 390 395 400 Asn
Thr Ser Ser Pro Arg Tyr Asp Ser His Cys Gly Leu Ser Asp Ala 405 410
415 Thr Pro Asn Ala Pro Glu Ala Gly Gln Trp Phe Gln Ala Tyr Phe Glu
420 425 430 Thr Leu Val Arg Asn Ala Ser Pro Pro Leu 435 440
88389PRTConiophora puteana 88Met Phe Ser Pro Val Val Leu Gly Ala
Leu Ala Ala Leu Leu Pro Ala 1 5 10 15 Ala Val Gln Ala Met Pro Ala
Ser Thr Gln Ala Arg Ala Ala Asp Ala 20 25 30 Thr Ala Asn Pro Tyr
Thr Gly Tyr Thr Ile Phe Lys Asn Pro Glu Tyr 35 40 45 Val Ala Glu
Val Gln Ala Ala Val Gln Gln Ile Ser Asp Ser Ser Leu 50 55 60 Ala
Ser Ala Ala Ala Gly Val Glu Asp Val Pro Val Phe Phe Trp Leu 65 70
75 80 Asp Gln Val Ala Lys Val Pro Asn Leu Thr Thr Tyr Leu Ala Ala
Ala 85 90 95 Asp Ala Glu Ala Lys Ser Ser Gly Ser Gln Gln Leu Phe
Gln Ile Val 100 105 110 Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala
Ala Ala Ser Asn Gly 115 120 125 Glu Phe Ser Ile Ser Asp Asn Gly Gln
Ala Asn Tyr Glu Asn Tyr Ile 130 135 140 Asp Gln Ile Val Ala Ser Ile
Lys Gln Tyr Pro Asp Val Arg Val Val 145 150 155 160 Ala Val Val Glu
Pro Asp Ser Met Ala Asn Leu Val Thr Asn Leu Ser 165 170 175 Val Gln
Lys Cys Ala Asp Ala Glu Ser Thr Tyr Lys Thr Cys Val Ala 180 185 190
Tyr Ala Ile Glu Gln Leu Ala Thr Val Gly Val Tyr Met Tyr Leu Asp 195
200 205 Ala Gly His Ala Gly Trp Leu Gly Trp Pro Ala Asn Leu Ser Pro
Ala 210 215 220 Ala Glu Leu Phe Ala Gln Met Tyr Ser Thr Thr Gly Ser
Ser Pro Tyr 225 230 235 240 Phe Arg Gly Leu Ala Thr Asn Val Ala Asn
Tyr Asn Ser Leu Thr Thr 245 250 255 Asp Ser Pro Asp Pro Ile Thr Ser
Gly Asp Ser Asn Tyr Asp Glu Leu 260 265 270 Leu Tyr Ile Glu Ala Leu
Ser Pro Leu Leu Val Asp Asn Gly Phe Pro 275 280 285 Ala Gln Phe Ile
Val Glu Gln Ala Arg Ser Gly Val Gln Asn Ile Arg 290 295 300 Ser Ala
Trp Gly Asp Trp Cys Asn Val Lys Gly Ala Gly Phe Gly Leu 305 310 315
320 Arg Pro Ser Thr Asp Thr Pro Ser Ser Leu Ile Asp Ser Ile Val Trp
325 330 335 Val Lys Pro Gly Gly Glu Ala Asp Gly Thr Ser Asn Ser Ser
Ala Ala 340 345 350 Arg Tyr Asp Tyr His Cys Ser Leu Ser Asp Ala Leu
Gln Pro Ala Pro 355 360 365 Glu Ala Gly Thr Trp Phe Gln Thr Tyr Phe
Glu Asp Leu Val Ser Gly 370 375 380 Ala Asn Pro Ala Phe 385
89454PRTCoprinopsis cinerea okayama 89Met Leu Lys Gly Ser Lys Phe
Phe Ala Leu Ser Leu Ala Leu Leu Pro 1 5 10 15 Ala Leu Val Gln Ala
Gln Arg Pro Leu Tyr Ala Gln Cys Gly Gly Thr 20 25 30 Gly Trp Thr
Gly Glu Thr Thr Cys Val Ser Gly Ala Val Cys Glu Val 35 40 45 Ile
Asn Gln Trp Tyr His Gln Cys Leu Pro Gly Ser Asn Gln Pro Gln 50 55
60 Pro Pro Val Thr Thr Gln Pro Pro Val Val Val Pro Thr Thr Ser Gln
65 70 75 80 Pro Pro Val Val Val Pro Thr Asn Pro Pro Gly Gly Thr Pro
Val Pro 85 90 95 Ser Thr Gly Asn Pro Phe Glu Gly Tyr Asp Ile Tyr
Leu Ser Pro Tyr 100 105 110 Tyr Ala Glu Glu Val Glu Ala Ala Ala Ala
Met Ile Asp Asp Pro Val 115 120 125 Leu Lys Ala Lys Ala Leu Lys Val
Lys Glu Ile Pro Thr Phe Ile Trp 130 135 140 Phe Asp Val Val Arg Lys
Thr Pro Asp Leu Gly Arg Tyr Leu Ala Asp 145 150 155 160 Ala Thr Ala
Ile Gln Gln Arg Thr Gly Arg Lys Gln Leu Val Gln Ile 165 170 175 Val
Val Tyr Asp Leu Pro Asp Arg Asp Cys Ala Ala Ala Ala Ser Asn 180 185
190 Gly Glu Phe Ser Leu Ala Asp Gly Gly Met Glu Lys Tyr Lys Asp Tyr
195 200 205 Val Asp Arg Leu Ala Ser Glu Ile Arg Lys Tyr Pro Asp Val
Arg Ile 210 215 220 Val Ala Val Ile Glu Pro Asp Ser Leu Ala Asn Met
Val Thr Asn Met 225 230 235 240 Asn Val Ala Lys Cys Arg Gly Ala Glu
Ala Ala Tyr Lys Glu Gly Val 245 250 255 Ile Tyr Ala Leu Arg Gln Leu
Ser Ala Leu Gly Val Tyr Ser Tyr Val 260 265 270 Asp Ala Gly His Ala
Gly Trp Leu Gly Trp Asn Ala Asn Leu Ala Pro 275 280 285 Ser Ala Arg
Leu Phe Ala Gln Ile Tyr Lys Asp Ala Gly Arg Ser Ala 290 295 300 Phe
Ile Arg Gly Leu Ala Thr Asn Val Ser Asn Tyr Asn Ala Leu Ser 305 310
315 320 Ala Thr Thr Arg Asp Pro Val Thr Gln Gly Asn Asp Asn Tyr Asp
Glu 325 330 335 Leu Arg Phe Ile Asn Ala Leu Ala Pro Leu Leu Arg Asn
Glu Gly Trp 340 345 350 Asp Ala Lys Phe Ile Val Asp Gln Gly Arg Ser
Gly Val Gln Asn Ile 355 360 365 Arg Gln Glu Trp Gly Asn Trp Cys Asn
Val Tyr Gly Ala Gly Phe Gly 370 375 380 Met Arg Pro Thr Leu Asn Thr
Pro Ser Ser Ala Ile Asp Ala Ile Val 385 390 395 400 Trp Ile Lys Pro
Gly Gly Glu Ala Asp Gly Thr Ser Asp Thr Ser Ala 405 410 415 Pro Arg
Tyr Asp Thr His Cys Gly Lys Ser Asp Ser His Lys Pro Ala 420 425 430
Pro Glu Ala Gly Thr Trp Phe Gln Glu Tyr Phe Val Asn Leu Val Lys 435
440 445 Asn Ala Asn Pro Pro Leu 450 90361PRTMoniliophthora
perniciosa 90Ile Pro Gly Ser Asp Pro Gly Asn Pro Gly Pro Thr Ser
Ser Ser Thr 1 5 10 15 Leu Ser Ser Thr Ala Ala Pro Pro Thr Asn Thr
Gln Ser Pro Val Glu 20 25 30 Asp Asn Pro Tyr Thr Gly Tyr Thr Ile
Tyr Leu Ser Pro Tyr Tyr Ala 35 40 45 Asp Glu Ile Asp Ala Ala Ala
Ala Lys Ile Thr Asp Pro Thr Leu Lys 50 55 60 Val Gln Ala Leu Lys
Val Lys Glu Ile Pro Thr Phe Ile Trp Phe Asp 65 70 75 80 Thr Thr Ala
Lys Leu Ser Thr Leu Glu Pro Tyr Leu Lys Asp Ala Ser 85 90 95 Ala
Lys Gly Lys Ala Glu Gly Lys Lys Tyr Leu Leu Gln Ile Val Val 100 105
110 Tyr Thr Leu Pro Glu Arg Asp Cys Ala Ala Leu Ala Ser Asn Gly Glu
115 120 125 Leu Ser Ile Asp Asn Gly Gly Glu Val Lys Ser Arg Glu Tyr
Ile Asp 130 135 140 Thr Met Val Ala Thr Ile Lys Lys Tyr Pro Asp Val
Arg Val Val Ala 145 150 155 160 Val Val Glu Pro Asp Ser Leu Ala Asn
Leu Val Thr Asn Leu Asn Val 165 170 175 Gln Lys Cys Ser Lys Ala Gln
Thr Ile Tyr Lys Thr Ser Thr Gln Tyr 180 185 190 Ala Leu Lys Gln Leu
Asp Thr Ala Gly Val Tyr Met Tyr Leu Asp Ala 195 200 205 Gly His Ala
Gly Trp Leu Gly Trp Pro Ala Asn Leu Thr Pro Thr Ala 210 215 220 Gln
Leu Phe Gln Gln Val Trp Gln Asp Ala Gly Ser Pro Lys Phe Val 225 230
235 240 Arg Gly Leu Ala Thr Asn Val Ala Asn Phe Asn Ala Leu Arg Ala
Ala 245 250 255 Ser Pro Asp Pro Val Thr Ser Gln Asn Pro Asn Tyr Asp
Glu Ile His 260 265 270 Tyr Ile Glu Gly Arg Ala Gly Gln Gln Asn Leu
Arg Lys Glu Trp Gly 275 280 285 Asp Trp Cys Asn Val Lys Gly Ala Gly
Phe Gly Thr Arg Pro Thr Thr 290 295 300 Asn Thr Gly Ser Ser Leu Ile
Asp Ser Ile Val Trp Val Lys Pro Gly 305 310 315 320 Gly Glu Ser Ala
Arg Phe Asp Ala Lys Cys Val Ser Ala Ser Ser His 325 330 335 Val Pro
Ala Pro Glu Ala Gly Thr Trp Phe Gln Glu Tyr Phe Glu Ala 340 345 350
Leu Val Arg Asn Ala Asn Pro Ala Leu 355 360 91460PRTPiromyces
rhizinflatus 91Met Lys Phe Ser Thr Leu Ile Gly Thr Leu Phe Ala Thr
Gly Ala Leu 1 5 10 15 Ala Ser Ser Cys His Arg Asp Tyr Pro Cys Cys
Asn Asp Cys Asn Val 20 25 30 Val Tyr Gln Asp Trp Glu Arg Asp Trp
Gly Val Leu Asn Gly Gln Glu 35 40 45 Trp Cys Phe Ile Asp Lys Asn
Arg Cys Asn Gly Gly Gly Tyr Cys Lys 50 55 60 Phe Glu Ser Leu Gly
Tyr Pro Cys Cys Asn Gly
Cys Asp Val Tyr Tyr 65 70 75 80 Thr Asp Asn Asp Gly Arg Trp Gly Val
Glu Asn Gly Asn Trp Cys Gly 85 90 95 Ile Arg Asp Asp Lys Cys Asn
Gly Tyr Gln Gln Pro Arg Thr Thr Thr 100 105 110 Thr Thr Arg Thr Thr
Thr Arg Thr Thr Thr Thr Gln Arg Pro Val Gln 115 120 125 Thr Asn Val
Ser Asp Asn Phe Phe Glu Asn Thr Leu Tyr Ser Asn Phe 130 135 140 Lys
Phe Gln Gly Glu Val Gln Ser Ser Ile Gln Lys Leu Ser Gly Asp 145 150
155 160 Met Ala Lys Lys Ala Glu Lys Val Lys Tyr Val Pro Thr Ala Val
Trp 165 170 175 Leu Ala Trp Glu Gly Ala Pro Arg Glu Val Pro Gln Tyr
Leu Asp Asp 180 185 190 Ala Gly Ser Lys Thr Val Val Phe Val Leu Tyr
Met Ile Pro Thr Arg 195 200 205 Asp Cys Asn Ala Asn Ala Ser Val Gly
Gly Ser Ala Thr Leu Glu Lys 210 215 220 Tyr Lys Gly Tyr Ile Asp Asn
Ile Tyr Asn Thr Phe Asn Gln Tyr Pro 225 230 235 240 Asn Ser Lys Ile
Val Met Ile Leu Glu Pro Asp Thr Ile Gly Asn Leu 245 250 255 Val Thr
Ala Asn Asn Ala Asn Cys Met Asn Val Gln Asn Leu His Lys 260 265 270
Gln Gly Leu Ala Tyr Ala Ile Ser Lys Phe Gly Thr Gln Lys Asn Val 275
280 285 Arg Val Tyr Leu Asp Ala Ala His Gly Ala Trp Leu Ser Ser His
Ala 290 295 300 Asp Lys Thr Ala Gln Val Ile Lys Glu Ile Leu Asn Asn
Ala Gly Ser 305 310 315 320 Gly Lys Leu Arg Gly Ile Thr Thr Asn Val
Ser Asn Tyr Gln Thr Val 325 330 335 Asn Asp Glu Tyr Ser Tyr Gln Met
Arg Leu Asn Ser Ala Leu Gln Asn 340 345 350 Leu Gly Val Arg Asp Leu
His Tyr Ile Ile Asp Thr Ser Arg Asn Gly 355 360 365 Ala Asn Ile Ala
Gln Gln Phe Asn Gln Ser Gly Thr Trp Cys Asn Phe 370 375 380 Lys Gly
Ala Gly Leu Gly Ala Arg Pro Gln Ala Asn Pro Asp Ser Ser 385 390 395
400 Lys Pro Leu Leu Asp Ala Tyr Met Trp Ile Lys Thr Pro Gly Glu Ala
405 410 415 Asp Gly Ser Ser Ser Gly Ser Arg Ala Asp Pro Val Cys Gly
Arg Trp 420 425 430 Asp Ser Leu Gln Gly Ala Pro Asp Ala Gly Ser Trp
Phe His Asp Tyr 435 440 445 Phe Val Met Leu Leu Gln Asn Ala Asn Pro
Pro Phe 450 455 460 92518PRTPiromyces rhizinflatus 92Met Lys Phe
Ile Val Cys Ala Ser Ile Leu Ser Leu Gly Leu Phe Lys 1 5 10 15 Phe
Ala Asp Ala Ala Cys Ala Gly Pro Tyr Ala Gln Cys Gly Gly Asn 20 25
30 Asn Phe Asn Gly Glu Asn Cys Cys Gln Ser Gly Tyr Lys Cys Val Ala
35 40 45 Ile Asn Glu Trp Tyr Ser Gln Cys Gln Glu Gly Ala Ala Glu
Pro Glu 50 55 60 Pro Val Pro Gln Ser Ser Ala Ala Asp Asp Gln Trp
Asn Asn Asn Asn 65 70 75 80 Gln Trp Asn Asn Asn Gln Gln Asn Asn Gln
Trp Asn Asn Pro Trp Asp 85 90 95 Asn Asn Asn Asn Gln Trp Asn Asn
Asn Gln Trp Asn Asn Asn Gln Gln 100 105 110 Asn Asn Gln Trp Asn Asn
Asn Gln Gln Gln Asn Asn Gln Trp Asp Asn 115 120 125 Asn Asn Gln Trp
Asn Asn Asn Gln Trp Asn Asn Asn Gln Gln Gln Asn 130 135 140 Asn Gln
Gln Gln Asn Asn Gln Gln Gln Asn Asn Gln Trp Asn Asn Asn 145 150 155
160 Asn Gln Trp Asn Asn Asn Gln Gln Gln Asn Asn Gln Gln Gln Asn Asn
165 170 175 Gln Ala Pro Ala Gln Ser Asn Gly Gly Gly Ala Ser Gly Ser
Ser Gln 180 185 190 Asn Phe Phe Thr Asn Glu Ile Tyr Ala Asn Pro Arg
Phe Ile Glu Glu 195 200 205 Ile Asp Ser Ser Ile Pro Lys Leu Thr Pro
Glu Leu Ala Ala Lys Ala 210 215 220 Glu Lys Val Lys Gln Val Pro Thr
Ala Val Trp Leu Ala Trp Asp Gly 225 230 235 240 Ala Pro Gly Glu Val
Glu Gly His Leu Lys Ala Ala Gly Ser Lys Thr 245 250 255 Val Val Phe
Ile Leu Tyr Met Ile Pro Thr Arg Asp Cys Asn Ser Asn 260 265 270 Ala
Ser Ala Gly Gly Ala Ser Asp Leu Asn Lys Tyr Lys Gly Tyr Val 275 280
285 Asp Asp Ile Ala Gly Thr Ile Lys Ser His Pro Glu Ser Lys Val Val
290 295 300 Met Ile Val Glu Pro Asp Thr Leu Gly Asn Leu Val Thr Gly
Ser Ser 305 310 315 320 Glu Ala Cys Lys Asn Val His Ser Leu His Lys
Ser Ala Leu Ser Tyr 325 330 335 Ala Val Asn Val Phe Gly Ala Met Ser
Asn Val Ser Val Tyr Leu Asp 340 345 350 Ala Ala His Gly Lys Trp Leu
Gly Gly Ser Thr Asp Lys Val Ala Ser 355 360 365 Val Leu Lys Glu Ile
Leu Asp Asn Ala Pro Asn Gly Lys Ile Arg Gly 370 375 380 Leu Ser Thr
Asn Val Ser Asn Tyr Gln Pro Leu Ser Ser Glu Ala Gln 385 390 395 400
Tyr His Gln Gly Leu Ser Ser Ala Leu Ala Ala Val Gly Tyr Pro Gly 405
410 415 Met Lys Phe Val Val Asp Thr Gly Arg Asn Gly Val Asp Val Ser
Ser 420 425 430 Thr Phe Ser Ile Asn Glu Thr Trp Cys Asn Phe Val Gly
Thr Gly Phe 435 440 445 Gly Glu Arg Pro Gln Gly Asn Pro Ser Gly Tyr
Pro Leu Leu Asp Ala 450 455 460 Phe Met Trp Leu Lys Thr Pro Gly Glu
Ala Asp Gly Ser Ala Thr Gly 465 470 475 480 Ser Arg Ala Asp Pro Val
Cys Ala Arg Gln Asp Ser Leu Gln Gly Ala 485 490 495 Pro Asp Ala Gly
Gln Trp Phe His Glu Tyr Phe Val Gln Leu Leu Glu 500 505 510 Asn Ala
Lys Pro Gly Phe 515 93376PRTPiromyces rhizinflatus 93Glu Thr Leu
Pro Gln Gln Ser Asn Ser Ala Lys Thr Leu Pro Gln Gln 1 5 10 15 Ser
Asp Ser Ala Lys Thr Ile Pro Gln Pro Thr Ser Ala Glu Ser Gln 20 25
30 Thr Ser Lys Thr Leu Pro Gln Thr Gly Gly Ser Gly Asn Gly Ser Ser
35 40 45 Gln Asn Phe Phe Leu Asn Glu Ile Tyr Ala Asn Pro Lys Phe
Ile Glu 50 55 60 Glu Val Glu Asp Ser Ile Glu Lys Leu Thr Pro Glu
Leu Gln Ala Lys 65 70 75 80 Ala Glu Lys Val Lys Asp Val Pro Thr Ala
Val Trp Leu Ala Trp Asp 85 90 95 Gly Ser Pro Gly Glu Val Glu Gly
His Leu Val Ala Ala Gly Ser Lys 100 105 110 Thr Val Val Phe Leu Leu
Tyr Met Ile Pro Thr Arg Asp Cys Asn Ser 115 120 125 Asn Ala Ser Ala
Gly Gly Ala Ala Ser Leu Asp Lys Tyr Lys Gly Tyr 130 135 140 Ile Asp
Asp Ile Ser Asn Thr Ile Lys Ser His Pro Glu Ser Lys Val 145 150 155
160 Val Met Val Val Glu Pro Asp Thr Leu Gly Asn Leu Val Thr Gly Asn
165 170 175 Ser Glu Ala Cys Lys Asn Val His Thr Leu His Lys Asn Ala
Leu Ser 180 185 190 Tyr Ala Val Asp Val Phe Gly Ala Met Ser Asn Val
Ser Val Tyr Leu 195 200 205 Asp Ala Ala His Gly Met Trp Leu Gly Pro
His Thr Asp Lys Val Ala 210 215 220 Ser Val Ile Lys Glu Ile Leu Asn
Asn Ala Pro Asn Gly Lys Ile Arg 225 230 235 240 Gly Leu Ser Thr Asn
Val Ser Asn Tyr Gln Pro Val Ser Ser Glu Tyr 245 250 255 Gln Tyr His
Gln Lys Leu Ala Ala Ser Leu Ala Ala Val Gly Val Asn 260 265 270 Asp
Val His Phe Ile Val Asp Thr Gly Arg Ser Gly Val Asp Val Thr 275 280
285 Glu Thr Phe Ser Lys Gln Gln Thr Trp Cys Asn Phe Ile Gly Ala Gly
290 295 300 Leu Gly Pro Arg Pro Gln Gly Asn Pro Asp Ala Ser Met Pro
Leu Leu 305 310 315 320 Asp Ala Tyr Met Trp Leu Lys Thr Pro Gly Glu
Ala Asp Gly Ser Ala 325 330 335 Val Gly Asp Arg Ala Asp Pro Val Cys
Ser His Glu Asp Ser Leu Gln 340 345 350 Val Ala Pro Asp Ala Gly Gln
Trp Phe His Asp Tyr Phe Val Leu Leu 355 360 365 Leu Lys Asn Ala Asn
Pro Pro Phe 370 375 94463PRTPiromyces rhizinflatus 94Met Lys Phe
Leu Ser Leu Ile Gly Thr Leu Phe Ala Thr Gly Ala Leu 1 5 10 15 Ala
Ser Gln Cys His Pro Asn Trp Pro Cys Cys Leu Asn Cys Asp Val 20 25
30 Val Tyr Gln Asp Gly Glu Gly Asp Trp Gly Val Leu Asn Asn Asp Trp
35 40 45 Cys Phe Ile Ser Lys Ser Arg Cys Gly Asn Asn Asn Asn Asn
Asn Asn 50 55 60 Gly Tyr Cys Lys Phe Gln Ser Leu Gly Tyr Pro Cys
Cys Ser Gly Cys 65 70 75 80 Gln Val Val Tyr Thr Asp Gly Asp Gly Asn
Trp Gly Val Glu Asn Gly 85 90 95 Asn Trp Cys Gly Ile Arg Asp Glu
Gln Cys Gly Gly Gly Asn Asn Trp 100 105 110 Gln Gln Pro Thr Thr Thr
Arg Thr Thr Thr Arg Thr Thr Thr Arg Thr 115 120 125 Gln Asn Asn Arg
Pro Thr Ser Asp Asn Phe Phe Asp Asn Thr Leu Tyr 130 135 140 Ser Asn
Tyr Lys Phe Gln Asp Glu Val Gln Ser Ser Ile Asn Lys Leu 145 150 155
160 Ser Gly Gln Met Ala Glu Lys Ala Lys Lys Val Lys Tyr Val Pro Thr
165 170 175 Ala Ala Trp Leu Ala Trp Ser Gly Ala Pro Asp Glu Val Pro
Arg Tyr 180 185 190 Leu Gln Glu Ala Gly Ser Asp Thr Val Val Phe Val
Leu Tyr Met Ile 195 200 205 Pro Thr Arg Asp Cys Asn Ala Asn Ala Ser
Ala Gly Gly Ser Ala Asn 210 215 220 Leu Asp Thr Tyr Lys Gly Tyr Val
Asn Lys Ile Tyr Asn Thr Ile Asn 225 230 235 240 Gln Tyr Pro Asn Ser
Arg Ile Val Met Ile Leu Glu Pro Asp Thr Ile 245 250 255 Gly Asn Leu
Val Thr Ala Asn Asn Gln Asn Cys Gln Asn Val Gln Asn 260 265 270 Leu
His Lys Asn Ala Leu Ala Tyr Ala Ile Ser Lys Phe Gly Thr Gln 275 280
285 Ser Asn Val Ser Val Tyr Leu Asp Ala Ala His Gly Ala Trp Leu Ser
290 295 300 Pro His Ala Asp Lys Thr Ala Gln Val Ile Lys Glu Ile Leu
Ser Lys 305 310 315 320 Ala Gly Asn Gly Lys Ile Arg Gly Ile Ser Thr
Asn Val Ser Asn Tyr 325 330 335 Gln Thr Ile Asp Asp Glu Tyr Asn Tyr
His Gln Arg Leu Asn Ser Ala 340 345 350 Leu Gln Asn Leu Gly Val Ser
Asn Met His Phe Ile Val Asp Thr Ser 355 360 365 Arg Asn Gly Ala Asn
Ile Ala Ser Gln Phe Asn Gln Ser Gly Thr Trp 370 375 380 Cys Asn Phe
Lys Gly Ala Gly Leu Gly Gln Arg Pro Lys Gly Ser Pro 385 390 395 400
Asp Pro Ser Lys Pro Leu Leu Asp Ala Tyr Met Trp Ile Lys Thr Pro 405
410 415 Gly Glu Ala Asp Gly Ser Ser Ser Gly Ala Arg Ala Asp Pro Val
Cys 420 425 430 Gly Arg Trp Asp Ser Leu Gln Gly Ala Pro Asp Ala Gly
Ser Trp Phe 435 440 445 His Asp Tyr Phe Val Met Leu Leu Gln Asn Ala
Asn Pro Ser Phe 450 455 460 95510PRTNeocallimastix frontalis 95Met
Lys Phe Ile Val Phe Ala Ser Ile Leu Ser Ser Gly Ile Ile Lys 1 5 10
15 Leu Ser Asn Ala Ala Cys Gly Gly Pro Tyr Ala Gln Cys Gly Gly Ser
20 25 30 Gly Phe Ser Gly Glu Ala Cys Cys Gln Asp Gly Tyr Lys Cys
Val Ala 35 40 45 Met Asn Glu Trp Tyr Ser Gln Cys Gln Ala Gly Ser
Asp Ala Pro Ala 50 55 60 Asn Ser Ala Ala Pro Val Asn Ser Ala Val
Gly Asn Asp Asn Asn Asn 65 70 75 80 Asn Asn Asn Gln Trp Asn Asn Gln
Trp Asn Asn Asn Gly Trp Asn Trp 85 90 95 Gly Asn Gly Asn Asn Glu
Ala Gln Asn Pro Trp Asn Asn Asn Gly Trp 100 105 110 Ser Trp Glu Gly
Gly Asn Asn Gly Asn Gln Asp Gln Asn Gln Trp Asp 115 120 125 Asn Asn
Gly Trp Pro Trp Gly Gly Asn Asn Gly Asn Gln Gly Pro Val 130 135 140
Gln Val Asn Ile Gly Glu Asn Asn Asn Gln Asn Gln Asn Pro Ala Asn 145
150 155 160 Asp Ala Pro Ala Pro Pro Pro Ala Gln Gly Gln Ala Pro Ala
Pro Ala 165 170 175 Pro Ala Ala Ala Ala Gly Gly Ser Gly Ser Ser Gln
Asn Phe Phe Gln 180 185 190 Asn Glu Ile Tyr Ala Asn Pro Lys Phe Ile
Glu Glu Val Asp Ser Ser 195 200 205 Ile Ala Lys Leu Asp Gly Glu Leu
Lys Ala Lys Ala Glu Lys Val Lys 210 215 220 Ser Val Pro Thr Ala Val
Trp Leu Ala Trp Asp Gly Ala Pro Gly Glu 225 230 235 240 Val Ala Gln
His Leu Glu Ala Ala Gly Ser Lys Thr Val Val Phe Ile 245 250 255 Met
Tyr Met Ile Pro Thr Arg Asp Cys Asn Ala Asn Ala Ser Ala Gly 260 265
270 Gly Ala Ser Asn Leu Gln Thr Tyr Lys Gly Tyr Val Asp Ser Ile Ser
275 280 285 Asn Thr Ile Lys Lys Tyr Pro Asn Ser Lys Val Val Met Ile
Leu Glu 290 295 300 Pro Asp Thr Leu Gly Asn Leu Val Thr Ala Asn Ser
Glu Asn Cys Lys 305 310 315 320 Asn Val His Gln Leu His Lys Asp Ala
Leu Ser Tyr Gly Val Asn Val 325 330 335 Phe Gly Ser Met Ser Asn Val
Ser Val Tyr Leu Asp Ala Ala His Gly 340 345 350 Ala Trp Leu Gly Asp
Ser Thr Asp Lys Val Ala Ala Val Val Lys Glu 355 360 365 Ile Leu Ser
Asn Ala Pro Asn Gly Lys Ile Arg Gly Leu Ser Thr Asn 370 375 380 Ile
Ser Asn Tyr Gln Pro Val Asp Ser Glu Tyr Gly Tyr His Gln Lys 385 390
395 400 Leu Ala Ser Ala Leu Ser Ala Ala Gly Tyr Pro Asp Met His Phe
Val 405 410 415 Val Asp Thr Gly Arg Asp Gly Val Ala Ile Ser Ser Gly
Thr Trp Cys 420 425 430 Asn Leu Ile Gly Thr Gly Phe Gly Glu Arg Pro
Lys Gly Asn Pro Asn 435 440 445 Pro Gly Met Pro Leu Leu Asp Ala Tyr
Met Trp Leu Lys Thr Pro Gly 450 455 460 Glu Ala Asp Gly Ser Ser Thr
Gly Ala Arg Ala Asp Pro Val Cys Ala 465 470 475 480 Lys Ser Asp Ser
Leu Pro Gly Ala Pro Asp Ala Gly Gln Trp Phe His 485 490 495 Asp Tyr
Phe Val Gln Leu Leu Lys Asn Ala Lys Pro Ala Phe 500 505 510
96459PRTOrpinomyces sp. PC-2 96Met Lys Phe Ser Thr Val Leu Ala Thr
Leu Phe Ala Thr Gly Ala Leu 1 5 10 15 Ala Ser Glu Cys His Trp Gln
Tyr Pro Cys Cys Lys Asp Cys Thr Val 20 25
30 Tyr Tyr Thr Asp Thr Glu Gly Lys Trp Gly Val Leu Asn Asn Asp Trp
35 40 45 Cys Met Ile Asp Asn Arg Arg Cys Ser Ser Asn Asn Asn Asn
Cys Ser 50 55 60 Ser Ser Ile Thr Ser Gln Gly Tyr Pro Cys Cys Ser
Asn Asn Asn Cys 65 70 75 80 Lys Val Glu Tyr Thr Asp Asn Asp Gly Lys
Trp Gly Val Glu Asn Asn 85 90 95 Asn Trp Cys Gly Ile Ser Asn Ser
Cys Gly Gly Gly Gln Gln Gln Gln 100 105 110 Pro Thr Gln Pro Thr Gln
Pro Thr Gln Pro Gln Gln Pro Thr Gln Pro 115 120 125 Ser Ser Asp Asn
Phe Phe Glu Asn Glu Ile Tyr Ser Asn Tyr Lys Phe 130 135 140 Gln Gly
Glu Val Asp Ile Ser Ile Lys Lys Leu Asn Gly Asp Leu Lys 145 150 155
160 Ala Lys Ala Glu Lys Val Lys Tyr Val Pro Thr Ala Val Trp Leu Ala
165 170 175 Trp Asp Gly Ala Pro Gln Glu Val Pro Arg Tyr Leu Gln Glu
Ala Gly 180 185 190 Asn Lys Thr Val Val Phe Val Leu Tyr Met Ile Pro
Thr Arg Asp Cys 195 200 205 Gly Ala Asn Ala Ser Ala Gly Gly Ser Ala
Thr Ile Asp Lys Tyr Lys 210 215 220 Gly Tyr Ile Asn Asn Ile Tyr Asn
Thr Ser Asn Gln Tyr Lys Asn Ser 225 230 235 240 Lys Ile Val Met Ile
Leu Glu Pro Asp Thr Ile Gly Asn Leu Val Thr 245 250 255 Asn Asn Asn
Asp Asn Cys Arg Asn Val Arg Asn Met His Lys Gln Ala 260 265 270 Leu
Ser Tyr Ala Ile Ser Lys Phe Gly Thr Gln Ser His Val Lys Val 275 280
285 Tyr Leu Asp Ala Ala His Gly Ala Trp Leu Asn Gln Tyr Ala Asp Gln
290 295 300 Thr Ala Asn Val Ile Lys Glu Ile Leu Asn Asn Ala Gly Ser
Gly Lys 305 310 315 320 Leu Arg Gly Ile Ser Thr Asn Val Ser Asn Tyr
Gln Ser Ile Glu Ser 325 330 335 Glu Tyr Lys Tyr His Gln Asn Leu Asn
Arg Ala Leu Glu Ser Lys Gly 340 345 350 Val Arg Gly Leu Lys Phe Ile
Val Asp Thr Ser Arg Asn Gly Ala Asn 355 360 365 Val Glu Gly Ala Phe
Asn Ala Ser Gly Thr Trp Cys Asn Phe Lys Gly 370 375 380 Ala Gly Leu
Gly Gln Arg Pro Lys Gly Asn Pro Asn Pro Gly Ser Met 385 390 395 400
Pro Leu Leu Asp Ala Tyr Met Trp Ile Lys Thr Pro Gly Glu Ala Asp 405
410 415 Gly Ser Ser Gln Gly Ser Arg Ala Asp Pro Val Cys Ala Arg Gly
Asp 420 425 430 Ser Leu Gln Gly Ala Pro Asp Ala Gly Ser Trp Phe His
Glu Tyr Phe 435 440 445 Thr Met Leu Ile Gln Asn Ala Asn Pro Pro Phe
450 455 97345PRTPiromyces rhizinflatus 97Thr Ala Val Trp Leu Ala
Trp Asp Gly Ala Pro Gly Glu Val Glu Gly 1 5 10 15 His Leu Lys Asn
Ala Gly Asp Lys Thr Ile Val Phe Ile Leu Tyr Met 20 25 30 Ile Pro
Thr Arg Asp Cys Asn Ser Lys Ala Ser Ala Gly Gly Ala Ser 35 40 45
Asn Leu Lys Lys Tyr Gln Gly Tyr Val Asp Ser Ile Ala Asn Thr Ile 50
55 60 Ser Lys His Pro Glu Ser Lys Val Val Met Val Ile Glu Pro Asp
Thr 65 70 75 80 Leu Gly Asn Leu Ile Thr Gly Glu Thr Glu Glu Cys Lys
Thr Val His 85 90 95 Thr Leu His Lys Asp Ala Leu Ala Tyr Ala Val
Asn Val Phe Gly Asp 100 105 110 Met Ser Asn Val Ser Ala Tyr Leu Asp
Ala Ala His Gly Lys Trp Leu 115 120 125 Gly Trp Ala Ala Gly Lys Thr
Ala Ala Val Ile Lys Glu Ile Leu Asp 130 135 140 Asn Ala Pro Asn Gly
Asn Ile Arg Gly Phe Ser Thr Asn Val Ser Asn 145 150 155 160 Tyr Gln
Pro Ile Glu Ser Glu Tyr Glu Tyr His Glu Lys Leu Asn Ala 165 170 175
Ala Leu Glu Asp Leu Gly Ile Thr Gly Lys Lys Phe Ile Val Asp Thr 180
185 190 Gly Arg Ser Gly Val Asp Val Thr Glu Glu Phe Asn Leu Asn Gln
Thr 195 200 205 Trp Cys Asn Leu Ile Tyr Ala Gly Leu Gly Glu Pro Ser
Arg Gly Ser 210 215 220 Pro Asp Pro Glu Lys Phe Pro Leu Leu Asp Ala
Tyr Phe Trp Leu Lys 225 230 235 240 Pro Pro Gly Glu Ala Asp Gly Ser
Asp Thr Gly Ser Arg Ala Asp Pro 245 250 255 Val Cys Gly Arg Glu Asp
Ser Phe Pro Gly Ala Pro Asp Ala Gly Ser 260 265 270 Trp Phe Ser Glu
Tyr Phe Ala Ser Met Leu Glu Lys Ser Pro Phe Tyr 275 280 285 Gly Glu
Gly Ile Glu Glu Glu Pro Glu Glu Pro Glu Glu Pro Leu Cys 290 295 300
Ser Gln Lys Phe Leu Asp Gln Gly Tyr Gln Cys Cys Ser Gln Cys Gly 305
310 315 320 Thr Ile Tyr Tyr Val Asp Asp Ala Gly Asn Trp Gly Val Glu
Asn Asn 325 330 335 Glu Trp Cys Gly Leu Pro Glu Asp Cys 340 345
98457PRTMicromonospora cellulolyticum 98Val Ala Ile Leu Ser Ala Arg
Arg Arg Ser Ala Ala Ile Ser Val Thr 1 5 10 15 Ala Val Ala Gly Leu
Ala Ala Ala Gly Val Leu Arg Val Gly Gly Val 20 25 30 Ala Gly Thr
Val Ser Gly Ser Leu Tyr Arg Asp Pro Ser Ser Ala Val 35 40 45 Val
Arg Trp Val Ala Ala Asn Pro Gly Asp Phe Arg Ala Ala Val Ile 50 55
60 Arg Glu Lys Ile Ala Ser Gln Pro Gln Ala Arg Trp Tyr Ala Asn Phe
65 70 75 80 Asn Pro Ser Thr Ile Gln Ser Glu Val Ser Ala Phe Ile Gly
Ala Ala 85 90 95 Asn Ser Ala Gln Gln Ile Pro Val Leu Ser Val Tyr
Glu Ile Thr Asn 100 105 110 Arg Asp Cys Gly Gly Ala His Ala Gly Gly
Ala Pro Asp Leu Asn Gln 115 120 125 Tyr Gln Thr Trp Val Ser Asn Phe
Ala Arg Gly Leu Gly Asn Gln Thr 130 135 140 Val Leu Ile Ile Leu Glu
Thr Asp Ser Leu Ala Leu Gln Thr Cys Leu 145 150 155 160 Ser Thr Ser
Glu Leu Asn Ala Arg Asn Gln Ala Leu Ser Thr Ala Thr 165 170 175 Gln
Thr Ile Lys Ser Ala Asn Pro Asn Ala Lys Val Tyr Leu Asp Gly 180 185
190 Gly His Ser Thr Trp Asn Ser Ala Asn Asp Thr Ala Asn Arg Leu Arg
195 200 205 Ala Ala Gly Val Gln Tyr Ala Asp Gly Phe Phe Thr Asn Val
Ser Asn 210 215 220 Phe Asn Pro Thr Ser Ser Glu Ala Asn Phe Gly Arg
Ala Val Ile Ser 225 230 235 240 Ala Leu Asn Gly Met Gly Ile Ser Gly
Lys Arg Gln Val Ile Asp Thr 245 250 255 Ser Arg Asn Gly Gly Ala Ala
Gly Asp Trp Cys Ala Asp Asp Asn Thr 260 265 270 Asp Arg Arg Ile Gly
Gln Tyr Pro Thr Thr Asn Thr Gly Asp Ala Asn 275 280 285 Ile Asp Ala
Tyr Leu Trp Val Lys Pro Pro Gly Glu Ala Asp Gly Cys 290 295 300 Ala
Thr Arg Gly Ser Phe Gln Pro Asp Leu Ala Phe Ser Leu Ala Asn 305 310
315 320 Gly Val Pro Asn Pro Pro Thr Thr Ala Pro Pro Thr Thr Asn Arg
Ala 325 330 335 Asp Asp Arg Pro Pro Thr Thr Ala Pro Pro Thr Thr Asp
Thr Pro Thr 340 345 350 Thr Ala Pro Pro Thr Thr Pro Pro Pro Ala Gly
Asn Gly Leu Ser Ala 355 360 365 Ser Val Ala Ile Thr Gln Trp Asn Gly
Gly Phe Thr Ala Ser Val Asn 370 375 380 Val Thr Ala Gly Ser Ala Ile
Asn Gly Trp Thr Val Thr Val Ala Leu 385 390 395 400 Pro Gly Gly Ala
Ala Ile Thr Gly Thr Trp Asn Ala Gln Ala Ser Gly 405 410 415 Thr Ser
Gly Thr Val Arg Phe Thr Asn Val Gly Tyr Asn Gly Gln Val 420 425 430
Gly Ala Gly Gln Thr Thr Asn Phe Gly Phe Gln Gly Thr Gly Thr Gly 435
440 445 Gln Gly Ala Thr Ala Thr Cys Ala Ala 450 455
99193PRTOrpinomyces sp. PC-2 99Thr Leu His Lys Asn Ala Leu Ser Tyr
Ala Val Asn Val Phe Gly Ser 1 5 10 15 Met Lys Asn Val Ser Val Tyr
Leu Asp Ala Ala His Gly Met Trp Leu 20 25 30 Ser Ala Val Ala Asp
Lys Thr Ala Ala Val Ile Lys Glu Val Leu Asp 35 40 45 Asn Ala Pro
Asn Gly Lys Ile Arg Gly Leu Ser Thr Asn Ile Ser Asn 50 55 60 Tyr
Gln Pro Val Tyr Ser Glu Tyr Lys Tyr His Glu Lys Leu Ser Ala 65 70
75 80 Glu Leu Glu Lys Leu Gly Val Ser Asp Ile His Phe Ile Val Asp
Thr 85 90 95 Gly Arg Asn Gly Val Asp Ile Thr Glu Thr Phe Ser Lys
Thr Gln Thr 100 105 110 Trp Cys Asn Phe Val Gly Thr Gly Phe Gly Glu
Arg Pro Gln Gly Asn 115 120 125 Pro Asp Pro Val Lys Met Pro Leu Leu
Asp Ala Tyr Met Trp Leu Lys 130 135 140 Thr Pro Gly Glu Ala Asp Gly
Ser Asp Thr Gly Ser Arg Ala Asp Pro 145 150 155 160 Val Cys Ala Arg
Glu Asp Ser Leu Pro Gly Ser Pro Asp Ala Gly Gln 165 170 175 Trp Phe
His Asp Tyr Phe Val Gln Leu Leu Glu Asn Ala Asn Pro Ala 180 185 190
Phe 100458PRTCatenulispora acidiphila 100Met Val Ala Thr Gln Leu
Gly Gly Val Ala Met Ala Gly Thr Ile Ala 1 5 10 15 Ser Gly Thr Gln
Phe Tyr Ala Asp Pro Asn Ser Gln Val Val Lys Trp 20 25 30 Asp Ala
Ala Asn Pro Gly Asp Ala Arg Glu Pro Ala Ile Ala Ser Gln 35 40 45
Ile Ala Ser Val Ser Gln Gly Ile Trp Phe Ser Asn Tyr Arg Pro Ser 50
55 60 Thr Val Gln Ser Asp Val Ser Ala Val Thr Thr Ala Ala Ala Ala
Ala 65 70 75 80 Gly Lys Thr Pro Val Leu Val Val Tyr Glu Ile Pro Asn
Arg Asp Cys 85 90 95 Gly Gly Ala Ser Ala Gly Gly Ala Pro Asp Ile
Ser Ser Tyr Glu Asn 100 105 110 Tyr Ile Gln Ser Phe Ala Asn Gly Leu
Gly Ser His Gln Val Ile Val 115 120 125 Ile Leu Glu Pro Asp Ser Leu
Ala Leu Gln Thr Cys Leu Ser Ser Gln 130 135 140 Gln Ala Thr Asp Arg
Asp Asn Ala Ile Ala Phe Ala Gly Ala His Leu 145 150 155 160 Lys Ser
Ala Asp Pro Ala Ala Lys Val Tyr Leu Asp Ala Gly His Ser 165 170 175
Ser Trp Asn Ser Pro Ser Ala Gln Ala Ala Ala Leu Asn Ala Ala Gly 180
185 190 Val Lys Thr Ser Ser Asp Gly Ile Phe Ser Asn Val Ser Asn Phe
Gln 195 200 205 Thr Thr Ala Ser Glu Val Ser Tyr Asp Lys Gln Val Leu
Ala Ala Leu 210 215 220 Gly Ser Pro Ser Asn Leu His Ile Val Val Asp
Thr Ser Arg Asn Gly 225 230 235 240 Asn Gly Pro Ala Gly Ser Ala Trp
Cys Asp Pro Ser Gly Arg Ala Leu 245 250 255 Gly Gln Ala Pro Thr Ala
Asn Thr Gly Asp Ala Ala Val Asp Ala Phe 260 265 270 Leu Trp Ile Lys
Pro Pro Gly Glu Ala Asp Gly Cys Ala Asp Ala Ala 275 280 285 Gly Thr
Phe Asp Pro Ala Leu Ala Tyr Ala Leu Ile Thr Asn Gly Gly 290 295 300
Gly Pro Pro Pro Thr Ser Pro Ser Ser Thr Pro Ser Thr Thr Pro Ser 305
310 315 320 Thr Thr Pro Ser Thr Thr Pro Ser Thr Thr Pro Ser Thr Thr
Pro Ser 325 330 335 Thr Thr Pro Ser Thr Pro Pro Ser Thr Thr Pro Ser
Ser Pro Pro Ser 340 345 350 Ser Ser Pro Ala Gly Cys Gln Val Thr Tyr
Thr Arg Thr Asn Glu Trp 355 360 365 Ala Gly Gly Phe Thr Ala Asn Val
Ser Ile Thr Ser Ser Lys Ala Leu 370 375 380 Ser Ser Trp Thr Val Gly
Phe Thr Tyr Gly Gly Asp Gln Gln Ile Thr 385 390 395 400 Asn Ser Trp
Asn Gly Asn His Thr Gln Ser Gly Arg Asn Val Thr Leu 405 410 415 Thr
Ser Leu Ser Tyr Asn Gly Ser Ile Gly Ala Gly Gln Thr Leu Thr 420 425
430 Gly Val Gly Val Gln Gly Thr Trp Thr Ser Ser Asp Ala Ala Pro Ser
435 440 445 Ala Phe Thr Leu Asn Gly Val Ala Cys His 450 455
101422PRTActinosynnema mirum 101Met Ala Gly Ala Ala Val Leu Val Thr
Gly Gly Gly Gln Thr Ala Thr 1 5 10 15 Ala Ala Asp Ser Ala Phe Tyr
Thr Asp Pro Gly Ser Ser Ser Ala Arg 20 25 30 Trp Val Ala Ala Asn
Pro Asn Asp Ser Arg Ala Ala Val Ile Arg Asp 35 40 45 Arg Val Ala
Ser Val Pro Gln Ala Lys Trp Phe Thr Thr Thr Asn Thr 50 55 60 Ser
Thr Val Arg Ser Glu Val Ser Ala Phe Val Gly Ala Ala Ala Ser 65 70
75 80 Ala Gly Lys Ile Pro Ile Leu Val Val Tyr Asn Ile Pro Asn Arg
Asp 85 90 95 Cys Gly Gly Ala Ser Gly Gly Gly Ala Pro Ser His Gln
Ala Tyr Arg 100 105 110 Ala Trp Val Asp Glu Val Ala Ala Gly Leu Gly
Gly Arg Pro Ala Ser 115 120 125 Ile Ile Leu Glu Pro Asp Val Leu Pro
Ile Met Ser Asn Cys Gln Ser 130 135 140 Ala Asp Gln Gln Asn Gln Thr
Lys Ala Ser Met Ser Tyr Ala Gly Arg 145 150 155 160 Lys Leu Lys Ser
Gly Ser Gly Gln Ala Lys Val Tyr Phe Asp Ile Gly 165 170 175 Asn Ser
Asp Trp Leu Ala Pro Ala Glu Ala Ala Asn Arg Leu Arg Gly 180 185 190
Ala Asp Val Ser Gly Ser Ser Asp Gly Ile Ala Ser Asn Val Ser Asn 195
200 205 Tyr Arg Ala Thr Gln Ala Glu Val Ser Tyr Thr Lys Ala Ile Leu
Asn 210 215 220 Ala Leu Gly Asp Gly Arg Leu Lys Ala Val Ile Asp Thr
Ser Arg Asn 225 230 235 240 Gly Asn Gly Pro Leu Gly Ser Glu Trp Cys
Asp Pro Pro Gly Arg Ala 245 250 255 Ile Gly Thr Pro Ser Thr Lys Asn
Thr Gly Asp Ser Gln Ile Asp Ala 260 265 270 Phe Leu Trp Val Lys Ile
Val Gly Glu Ala Asp Gly Cys Ile Ala Ser 275 280 285 Ala Gly Gln Phe
Val Pro Gln Arg Ala Tyr Asp Leu Ala Val Ala Ala 290 295 300 Gly Pro
Val Pro Thr Thr Thr Thr Thr Thr Pro Gly Gly Asn Pro Gly 305 310 315
320 Gly Gly Cys Ala Val Thr His Arg Val Val Ser Gln Trp Asn Gly Gly
325 330 335 Phe Thr Gly Glu Val Val Val Glu Asn Arg Gly Pro Ala Ile
Ser Ser 340 345 350 Trp Thr Leu Glu Phe Ser Ala Pro Gly Val Thr Val
Thr Gln Gly Trp 355 360 365 Asn Gly Thr Trp Thr Asp Thr Gly Asp Gly
Val Arg Val Val Asn Thr 370 375 380 Ala Trp Asn Gly Ala Leu Ala Ser
Gly Gly Arg Val Thr Ala Gly Tyr 385 390 395
400 Asn Ala Asn Tyr Gly Gly Gly Ala Pro Pro Phe Ser Ser Pro Thr Leu
405 410 415 Asn Gly Ala Ala Cys Ser 420 102444PRTActinosynnema
mirum 102Met Ala Ala Gly Ala Leu Ser Ser Ala Leu Val Ala Ala Ala
Thr Ala 1 5 10 15 Ile Ala Thr Gly Thr Ala Ser Pro Ala Ala Val Ala
Ala Asp Ser Glu 20 25 30 Phe Tyr Ser Asp Pro Ala Thr Ser Ala Ala
Arg Trp Val Ala Ala Asn 35 40 45 Pro Asn Asp Ser Arg Ala Ala Val
Ile Arg Asp Arg Val Ala Ser Val 50 55 60 Pro Gln Ala Lys Trp Phe
Thr Thr Thr Asn Thr Ser Thr Ile Arg Ala 65 70 75 80 Glu Val Asp Ala
His Thr Ser Ala Ala Ala Ser Ala Gly Lys Thr Pro 85 90 95 Ile Leu
Val Val Tyr Asn Ile Pro Asn Arg Asp Cys Gly Gly Ala Ser 100 105 110
Gly Gly Gly Ala Pro Ser His Gly Ala Tyr Arg Gln Trp Val Asp Gln 115
120 125 Phe Ala Ala Gly Leu Ala Gly Arg Pro Ala Ala Ile Ile Leu Glu
Pro 130 135 140 Asp Val Leu Pro Ile Met Ser Thr Cys Gln Ser Ala Ser
Gln Gln Ala 145 150 155 160 Glu Thr Arg Ala Ser Met Ala Tyr Ala Gly
Lys Ala Leu Lys Ala Ala 165 170 175 Ser Ser Gln Ala Lys Val Tyr Phe
Asp Ile Gly His Ser Ala Trp Leu 180 185 190 Thr Pro Ala Glu Ala Ala
Asn Arg Leu Arg Ala Ala Glu Val Ser Thr 195 200 205 Ser Ala Asp Gly
Ile Ala Thr Asn Val Ser Asn Tyr Arg Arg Thr Ala 210 215 220 Asp Glu
Val Ala Phe Ala Lys Ala Thr Leu Asn Ala Leu Gly Asp Gly 225 230 235
240 Arg Leu Lys Ala Val Val Asp Thr Ser Arg Asn Gly Asn Gly Pro Leu
245 250 255 Gly Ser Glu Trp Cys Asp Pro Pro Gly Arg Ala Ile Gly Thr
Pro Ser 260 265 270 Thr Arg Asn Thr Gly Asp Pro Gln Ile Asp Ala Phe
Leu Trp Val Lys 275 280 285 Ile Pro Gly Glu Ala Asp Gly Cys Ile Ala
Gly Ala Gly Gln Phe Val 290 295 300 Pro Gln Arg Ala Tyr Asp Met Ala
Val Ala Ala Gly Pro Ala Pro Thr 305 310 315 320 Thr Thr Thr Thr Thr
Thr Thr Thr Thr Arg Val Thr Thr Thr Thr Thr 325 330 335 Thr Pro Pro
Pro Asn Gly Ala Ala Cys Val Val Arg His Arg Val Val 340 345 350 Ser
Ser Trp Ser Gly Gly His Thr Gly Glu Val Val Ile Glu Asn Arg 355 360
365 Gly Pro Ala Leu Gln Asn Trp Thr Leu Glu Phe Ser Ala Pro Gly Val
370 375 380 Ala Val Ser Gln Gly Trp Asn Gly Thr Trp Thr Asp Leu Gly
Asp Thr 385 390 395 400 Val Arg Val Thr Ser Ala Ser Trp Asn Gly Gly
Ile Ala Thr Gly Gly 405 410 415 Thr Ala Thr Thr Gly Tyr Ser Ala Ser
Phe Ser Gly Gly Thr Pro Pro 420 425 430 Phe Thr Ser Pro Val Leu Asn
Gly Thr Ala Cys Ala 435 440 103426PRTNocardiopsis dassonvillei
103Met Ser Ser Val Ser Ala Leu Ala Leu Gly Thr Ala Leu Leu Ala Thr
1 5 10 15 Ala Pro Ala Ser Ala Ala Asp Ser Glu Phe Tyr Val Asn Pro
Asn Thr 20 25 30 Ser Ala Ala Val Trp Val Glu Glu Asn Pro Asn Asp
Pro Arg Ala Asp 35 40 45 Val Ile Arg Asp Arg Ile Ala Ser Val Ala
Gln Ala Thr Trp Phe Thr 50 55 60 Gln Tyr Asn Pro Ala Glu Val Arg
Asp Asp Val Asp Ala Val Val Ser 65 70 75 80 Ala Ala Asp Ala Gln Gly
Gln Thr Pro Ile Leu Val Val Tyr Asn Ile 85 90 95 Pro Gly Arg Asp
Cys Gly Asn His Ser Gly Gly Gly Ala Pro Ser His 100 105 110 Asp Ala
Tyr Arg Ala Trp Val Asp Glu Val Ala Ala Gly Leu Glu Gly 115 120 125
Arg Ser Ala Thr Ile Val Leu Glu Pro Asp Ala Leu Pro Leu Val Ser 130
135 140 Gly Cys Ser Asp Pro Ser Glu Leu Leu Asp Ser Met Ala Tyr Ala
Gly 145 150 155 160 Lys Ala Leu Met Glu Gly Ser Ser Glu Ala Arg Val
Tyr Phe Asp Ile 165 170 175 Gly Asn Ser Ala Trp Leu Asp Pro Gln Glu
Ala Ala Gly Leu Leu Asn 180 185 190 Gly Ala Asp Val Ala Asn Ser Ala
His Gly Val Ala Thr Asn Thr Ser 195 200 205 Asn Tyr Asn Trp Thr His
Asp Glu Val Ala Phe Ala Glu Ala Val Ile 210 215 220 Ala Ala Thr Gly
Val Pro Gly Leu Gly Ala Val Ile Asp Thr Ser Arg 225 230 235 240 Asn
Gly Asn Gly Pro Ala Pro Gln Asn Glu Trp Cys Asp Pro Pro Gly 245 250
255 Arg Met Ile Gly Arg Pro Ser Thr Thr Asp Thr Gly Asn Pro Leu Ile
260 265 270 Asp Ala Phe Ile Trp Thr Lys Leu Pro Gly Glu Ala Asp Gly
Cys Ile 275 280 285 Ala Pro Ala Gly Gln Phe Val Pro Gln Ala Ala Tyr
Asp Met Ala Val 290 295 300 Asn Ala Pro Glu Tyr Pro Thr Asp Pro Gly
Glu Pro Thr Asp Pro Glu 305 310 315 320 Glu Pro Thr Asp Pro Pro Glu
Gly Glu Gly Cys Thr Ala Asp Tyr Arg 325 330 335 Val Val Ser Glu Trp
Gly Asn Gly Phe Gln Ala Ala Val Thr Val Thr 340 345 350 Ala Glu Asp
Ser Leu Ser Gly Trp Thr Val Thr Trp Thr Tyr Ala Asp 355 360 365 Gly
Gln Arg Phe Ser Gln Gly Trp Asn Ala Glu Phe Ser Ser Ser Gly 370 375
380 Ser Arg Val Thr Ala Ser Asp Leu Gly Trp Asn Gly Thr Leu Ser Ala
385 390 395 400 Gly Gly Ser Thr Glu Phe Gly Phe Thr Gly Thr His Gly
Gly Ser Asn 405 410 415 Gly Val Pro Glu Val Thr Cys Ser Ala Ala 420
425 104286PRTThermobifida fusca 104Asn Asp Ser Pro Phe Tyr Val Asn
Pro Asn Met Ser Ser Ala Glu Trp 1 5 10 15 Val Arg Asn Asn Pro Asn
Asp Pro Arg Thr Pro Val Ile Arg Asp Arg 20 25 30 Ile Ala Ser Val
Pro Gln Gly Thr Trp Phe Ala His His Asn Pro Gly 35 40 45 Gln Ile
Thr Gly Gln Val Asp Ala Leu Met Ser Ala Ala Gln Ala Ala 50 55 60
Gly Lys Ile Pro Ile Leu Val Val Ser Asn Ala Pro Gly Arg Asp Cys 65
70 75 80 Gly Asn His Ser Ser Gly Gly Ala Pro Ser His Ser Ala Tyr
Arg Ser 85 90 95 Trp Ile Asp Glu Phe Ala Ala Gly Leu Lys Asn Arg
Pro Ala Tyr Ile 100 105 110 Ile Val Glu Pro Asp Leu Ile Ser Leu Met
Ser Ser Cys Met Gln His 115 120 125 Val Gln Gln Glu Val Leu Glu Thr
Met Ala Tyr Ala Gly Lys Ala Leu 130 135 140 Lys Ala Gly Ser Ser Gln
Ala Arg Ile Tyr Phe Asp Ala Gly His Ser 145 150 155 160 Ala Trp His
Ser Pro Ala Gln Met Ala Ser Trp Leu Gln Gln Ala Asp 165 170 175 Ile
Ser Asn Ser Ala His Gly Ile Ala Thr Asn Thr Ser Asn Tyr Arg 180 185
190 Trp Thr Ala Asp Glu Val Ala Tyr Ala Lys Ala Val Leu Ser Ala Ile
195 200 205 Gly Asn Pro Ser Leu Arg Ala Val Ile Asp Thr Ser Arg Asn
Gly Asn 210 215 220 Gly Pro Ala Gly Asn Glu Trp Cys Asp Pro Ser Gly
Arg Ala Ile Gly 225 230 235 240 Thr Pro Ser Thr Thr Asn Thr Gly Asp
Pro Met Ile Asp Ala Phe Leu 245 250 255 Trp Ile Lys Leu Pro Gly Glu
Ala Asp Gly Cys Ile Ala Gly Ala Gly 260 265 270 Gln Phe Val Pro Gln
Ala Ala Tyr Glu Met Ala Ile Ala Ala 275 280 285
105448PRTMicromonospora sp. ATCC 39149 105Met Ala Ala Ser Ala Leu
Thr Ala Ala Val Ala Val Ser Ile Leu Gly 1 5 10 15 Gly Thr Pro Ala
Ser Ala Ala Asp Ser Ala Phe Tyr Val Asp Pro Gln 20 25 30 Ala Ser
Ala Ala Arg Trp Val Ala Ala Asn Pro Gly Asp Trp Arg Ala 35 40 45
Ala Val Ile Arg Asp Arg Ile Ala Ala Val Pro Gln Gly Arg Trp Phe 50
55 60 Thr Thr Thr Asn Thr Ser Thr Val Arg Ser Glu Val Asp Gln Phe
Val 65 70 75 80 Gly Ala Ala Ala Ala Ala Gly Lys Val Pro Ile Met Val
Val Tyr Asn 85 90 95 Ile Pro Asn Arg Asp Cys Ser Gly Ala Ser Gly
Gly Gly Ala Pro Ser 100 105 110 His Thr Ala Tyr Arg Gln Trp Val Asp
Gln Val Ala Ala Gly Leu Ala 115 120 125 Gly Arg Pro Ala Thr Ile Val
Leu Glu Pro Asp Val Leu Pro Ile Met 130 135 140 Thr Asn Cys Gln Asn
Ala Ser Gln Gln Ala Glu Thr Arg Ala Ser Met 145 150 155 160 Ala Tyr
Ala Gly Lys Lys Leu Lys Ser Gly Ser Ala Gln Ala Lys Val 165 170 175
Tyr Phe Asp Ala Gly Asn Ser Ala Trp Leu Ala Pro Ala Glu Ile Ala 180
185 190 Ser Arg Leu Asn Gly Ala Asp Ile Ala Asn Ser Ala Asp Gly Ile
Ser 195 200 205 Leu Asn Val Ser Asn Tyr Arg Thr Thr Ala Glu Ser Val
Ser Tyr Ala 210 215 220 Lys Gln Val Ile Ala Ala Thr Gly Val Ser Arg
Leu Lys Ala Val Ile 225 230 235 240 Asp Thr Ser Arg Asn Gly Asn Gly
Pro Leu Gly Ser Glu Trp Cys Asp 245 250 255 Pro Pro Gly Arg Ala Ile
Gly Thr Pro Ser Thr Thr Ala Thr Gly Asp 260 265 270 Ser Ala Ile Ala
Ala Tyr Leu Trp Val Lys Leu Pro Gly Glu Ala Asp 275 280 285 Gly Cys
Ile Ala Pro Ala Gly Gln Phe Val Pro Gln Arg Ala Tyr Asp 290 295 300
Leu Ala Ile Ala Ala Gly Pro Val Pro Thr Thr Ala Pro Pro Thr Thr 305
310 315 320 Ala Pro Pro Thr Thr Ala Pro Pro Thr Thr Ala Pro Pro Thr
Thr Ala 325 330 335 Pro Pro Thr Thr Pro Pro Asn Gly Ala Cys Lys Val
Thr Phe Thr Pro 340 345 350 Asn Thr Trp Ser Gly Gly Phe Thr Ala Glu
Leu Arg Val Thr Asn Gly 355 360 365 Gly Ser Ala Leu Asn Gly Trp Ser
Leu Ser Phe Gly Phe Gly Ser Gly 370 375 380 Ser Gly Val Arg Leu Thr
Ser Gly Trp Asn Gly Glu Trp Ser Gln Asn 385 390 395 400 Gly Asp Val
Phe Leu Val Arg Asn Ala Ala Trp Asn Gly Asn Leu Pro 405 410 415 Ala
Gly Gly Thr Leu Ser Val Gly Phe Gln Gly Thr Phe Ser Gly Ala 420 425
430 Ser Leu Pro Thr Ala Val Gly Phe Thr Leu Asn Gly Ser Arg Cys Asn
435 440 445 106456PRTThermobispora bispora 106Met Ser Arg Ile Arg
Arg Phe Leu Ala Thr Ala Leu Ala Ala Ala Thr 1 5 10 15 Ala Gly Val
Gly Ala Ile Val Thr Ala Ile Ala Ser Ala Gly Pro Ala 20 25 30 His
Ala Tyr Asp Ser Pro Phe Tyr Val Asp Pro Gln Ser Asn Ala Ala 35 40
45 Lys Trp Val Ala Ala Asn Pro Asn Asp Pro Arg Thr Pro Val Ile Arg
50 55 60 Asp Arg Ile Ala Ala Val Pro Thr Gly Arg Trp Phe Ala Asn
Tyr Asn 65 70 75 80 Pro Ser Thr Val Arg Ala Glu Val Asp Ala Tyr Val
Gly Ala Ala Ala 85 90 95 Ala Ala Gly Lys Ile Pro Ile Met Val Val
Tyr Ala Met Pro Asn Arg 100 105 110 Asp Cys Gly Gly Pro Ser Ala Gly
Gly Ala Pro Asn His Thr Ala Tyr 115 120 125 Arg Ala Trp Ile Asp Glu
Ile Ala Ala Gly Leu Arg Asn Arg Pro Ala 130 135 140 Val Ile Ile Leu
Glu Pro Asp Ala Leu Pro Ile Met Thr Asn Cys Met 145 150 155 160 Ser
Pro Ser Glu Gln Ala Glu Val Gln Ala Ser Ala Val Gly Ala Gly 165 170
175 Lys Lys Phe Lys Ala Ala Ser Ser Gln Ala Lys Val Tyr Phe Asp Ala
180 185 190 Gly His Asp Ala Trp Val Pro Ala Asp Glu Met Ala Ser Arg
Leu Arg 195 200 205 Gly Ala Asp Ile Ala Asn Ser Ala Asp Gly Ile Ala
Leu Asn Val Ser 210 215 220 Asn Tyr Arg Tyr Thr Ser Gly Leu Ile Ser
Tyr Ala Lys Ser Val Leu 225 230 235 240 Ser Ala Ile Gly Ala Ser His
Leu Arg Ala Val Ile Asp Thr Ser Arg 245 250 255 Asn Gly Asn Gly Pro
Leu Gly Ser Glu Trp Cys Asp Pro Pro Gly Arg 260 265 270 Ala Thr Gly
Thr Trp Ser Thr Thr Asp Thr Gly Asp Pro Ala Ile Asp 275 280 285 Ala
Phe Leu Trp Ile Lys Pro Pro Gly Glu Ala Asp Gly Cys Ile Ala 290 295
300 Thr Pro Gly Val Phe Val Pro Asp Arg Ala Tyr Glu Leu Ala Met Asn
305 310 315 320 Ala Ala Pro Pro Thr Tyr Ser Pro Ser Pro Thr Pro Ser
Thr Pro Ser 325 330 335 Pro Ser Pro Ser Gln Ser Asp Pro Gly Ser Pro
Ser Pro Ser Pro Ser 340 345 350 Gln Pro Pro Ala Gly Arg Ala Cys Glu
Ala Thr Tyr Ala Leu Val Asn 355 360 365 Gln Trp Pro Gly Gly Phe Gln
Ala Glu Val Thr Val Lys Asn Thr Gly 370 375 380 Ser Ser Pro Ile Asn
Gly Trp Thr Val Gln Trp Thr Leu Pro Ser Gly 385 390 395 400 Gln Ser
Ile Thr Gln Leu Trp Asn Gly Asp Leu Ser Thr Ser Gly Ser 405 410 415
Asn Val Thr Val Arg Asn Val Ser Trp Asn Gly Asn Val Pro Ala Gly 420
425 430 Gly Ser Thr Ser Phe Gly Phe Leu Gly Ser Gly Thr Gly Gln Leu
Ser 435 440 445 Ser Ser Ile Thr Cys Ser Ala Ser 450 455
1075PRTArtificial SequenceConsensus sequence 107Gly Glu Xaa Asp Gly
1 5
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