U.S. patent application number 15/029775 was filed with the patent office on 2016-09-29 for straw for the preservation of a predetermined dose of liquid-based substance, in particular pure or diluted animal semen; and set comprising it.
The applicant listed for this patent is IMV TECHNOLOGIES. Invention is credited to Olivier CARlON, Jean-Charles GORGES, Eric SCHMITT.
Application Number | 20160278905 15/029775 |
Document ID | / |
Family ID | 49667473 |
Filed Date | 2016-09-29 |
United States Patent
Application |
20160278905 |
Kind Code |
A1 |
SCHMITT; Eric ; et
al. |
September 29, 2016 |
Straw for the preservation of a predetermined dose of liquid-based
substance, in particular pure or diluted animal semen; and set
comprising it
Abstract
The invention relates to a straw comprising a tube (11) and a
gas-permeable and liquid-tight stopper (12), said stopper (12)
being arranged in the tube (11), close to an end (16). The
invention is characterised in that the stopper (12) comprises an
indicator component (20) having a first pre-determined colour in
the absence of previous contact with said substance (21) and a
second pre-determined colour, of a different shade from that of the
first colour, once it has been in contact with said substance (21).
The assembly comprises the straw (10) and a device (26) for
controlling the filling of the straw.
Inventors: |
SCHMITT; Eric;
(VILLAINES-LA-JUHEL, FR) ; GORGES; Jean-Charles;
(CHENAY, FR) ; CARlON; Olivier; (RAI, FR) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
IMV TECHNOLOGIES |
Saint Quen Sur Iton |
|
FR |
|
|
Family ID: |
49667473 |
Appl. No.: |
15/029775 |
Filed: |
October 13, 2014 |
PCT Filed: |
October 13, 2014 |
PCT NO: |
PCT/FR2014/052598 |
371 Date: |
April 15, 2016 |
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
G01N 2021/7786 20130101;
B01J 2220/49 20130101; A01N 1/0263 20130101; A61D 19/024 20130101;
G01N 21/78 20130101 |
International
Class: |
A61D 19/02 20060101
A61D019/02; G01N 21/78 20060101 G01N021/78; A01N 1/02 20060101
A01N001/02 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 15, 2013 |
FR |
1360037 |
Claims
1. A straw for the preservation of a predetermined dose of
liquid-based substance, comprising a tube (11) and a gas-permeable
liquid-tight stopper (12), which stopper (12) is disposed in the
tube (11) in the neighborhood of one end (16), which tube (11) and
which stopper (12) are configured for the stopper (12) to be able
to slide in the tube (11) towards the other end (17), wherein said
stopper (12) comprises an indicator component (20) having a first
predetermined color in the absence of prior contact with said
substance (21) and a second predetermined color, having a hue
different from the hue of the first color, when it has been in
contact with said substance (21).
2. A straw according to claim 1, wherein said indicator component
(20) emits light of which the spectrum comprises at least one peak
(22, 35, 37) having a crest (23, 36, 38) of predetermined
wavelength when it has been in contact with said substance and does
not comprise said peak (22, 35, 37) in the absence of prior contact
with said substance.
3. A straw according to claim 2, wherein said indicator component
(20) emits light of which the spectrum comprises said at least one
peak (22, 35, 37) in response to illumination by light comprising a
predetermined range of wavelengths that is offset relative to the
predetermined wavelength of said crest (23, 36, 38).
4. A straw according to claim 1, wherein said stopper (12) is
formed by two plugs (13, 14) made from a fibrous substance
enclosing a sealing agent (20) formed by a powder (15) transforming
on contact with said substance (21) into an impermeable paste or
gel (15') adhering to the wall of the tube (11) so that the stopper
(12) is liquid-tight, said indicator component comprising said
sealing agent (20).
5. A straw according to claim 4, wherein said powder (15) comprises
a powder of a salt that is non-fluorophore in the dry state and
fluorophore when it is dissolved in water.
6. A straw according to claim 5, wherein said salt forms part of
the group comprising a fluorescein salt, a Rhodamine B salt, a
Rhodamine 6G salt and a salt of Eriochrome.RTM. Cyanine R.
7. A straw according to claim 5, wherein said powder (15) comprises
between 1/100 and 1/100000 by weight of said salt powder.
8. A straw according to claim 5, wherein said powder (15) is formed
by said salt powder and by powder of material that polymerizes on
contact with water.
9. A straw according to claim 8, wherein said powder of material
that polymerizes on contact with water is alginate.
10. A straw according to claim 1, wherein said indicator component
comprises an indicator thread having a first predetermined color in
the absence of prior contact with said substance and a second
predetermined color, having a hue different from the first color
hue, when it has been in contact with said substance.
11. A straw according to claim 10, wherein said stopper is formed
by two plugs (13, 14) made from a braided fibrous substance
enclosing a sealing agent (20) formed by a powder (15) which, on
contact with a liquid, transforms into an impermeable paste or gel
(15') adhering to the wall of the tube so that the stopper is
liquid-tight, at least one of the two plugs (13, 14) comprising
said indicator thread.
12. A set comprising a straw according to claim 1 and a device (26)
for checking filling of said straw, wherein said checking device
26) comprises a detection member (27) for detecting whether said
indicator component (20) has taken on said hue of the second
color.
13. A set according to claim 12, wherein the indicator component
(20) of the stopper (12) of the straw (10) emits light of which the
spectrum comprises at least one peak (22, 35, 37) having a crest
(23, 36, 38) of predetermined wavelength when the indicator
component (20) has been in contact with said substance (21) and
does not comprise said peak (22, 35, 37) in the absence of prior
contact with said substance (21); and said detection member (27)
comprised by the checking device (26) is configured to detect the
absence from or the presence in the spectrum formed by the light
emitted by said indicator component (20), of said at least one peak
(22, 35, 37).
14. A set according to claim 13, wherein said indicator component
(20) of the stopper (12) of the straw (10) emits light of which the
spectrum comprises said at least one peak (22, 35, 37) in response
to illumination by light comprising a predetermined range of
wavelengths that is offset relative to the predetermined wavelength
of said crest (23, 36, 38); and said checking device (26) further
comprises an illuminating member (29) to illuminate said indicator
component (20) by light comprising said predetermined range of
wavelengths.
15. A set according to claim 12, wherein the checking device (26)
forms part of a filler machine (25) for straws and comprises a
processing unit (28) linked to a detection member (27) configured
to emit a stop signal for the filler machine (25) if the detection
member (27) detects a straw (10) in which the indicator component
(20) has not taken on said hue of the second color.
Description
[0001] The invention generally relates to the preservation of a
predetermined dose of liquid-based substance, in particular pure or
diluted animal semen; and more particularly to the straws for
performing such preservation.
[0002] It is known that such a straw comprises a tube and a stopper
disposed in the tube. The stopper is usually of the three-part type
originally described in French patent 995 878, corresponding to
British patent 669 265, i.e. formed by two plugs made from a
fibrous substance enclosing a powder which, on contact with a
liquid, transforms into an impermeable paste or gel adhering to the
wall of the tube so that the stopper is liquid-tight.
[0003] Similar but improved stoppers are described by the French
patent applications 2 824 255 and 2 824 256.
[0004] Stoppers of another type are also known, for example a
stopper made from a single-piece cylinder of hydrophobic
microporous material described by European patent application 0 873
726 or a stopper made from a single-piece cylinder of sintered
self-sealing microporous material as described by PCT application
WO 2010/070533.
[0005] In the initial state, the stopper is disposed in the
neighborhood of one of the ends of the tube and it is provided that
in the filled state, the dose of liquid substance which must be
preserved in the straw is disposed between the stopper and the
other end of the tube (the end furthest from the stopper). The tube
and the stopper are configured for the stopper to be able to slide
in the tube towards the end that is initially furthest from the
stopper.
[0006] To fill the straw, the end closest to the stopper is placed
in communication with a vacuum source while the furthest end of the
tube is placed in communication with a vessel containing the
substance to be introduced into the straw.
[0007] The air initially contained between the stopper and the
furthest end of the tube is sucked through the stopper while the
substance moves forward into the tube until it meets the
stopper.
[0008] If necessary, after filling, the straw is welded close to
one or both of its ends and is stored cold.
[0009] In order to empty the straw, if necessary after cutting the
welded end portions and thawing, a rod is inserted into the tube
via the end closest to the stopper, until it bears against the
stopper. Using this rod, the plug is made to slide in the manner of
a piston towards the end furthest from the stopper, which causes
the expulsion of the dose of substance which had been introduced
into the straw.
[0010] The invention is directed to making it possible to detect,
simply, conveniently and economically, whether the straw has been
correctly filled.
[0011] To that end the invention provides a straw for the
preservation of a predetermined dose of liquid-based substance,
comprising a tube and a gas- permeable liquid-tight stopper, which
stopper is disposed in the tube in the neighborhood of one end,
characterized in that: said stopper comprises an indicator
component having a first predetermined color in the absence of
prior contact with said substance and a second predetermined color,
having a hue different from the hue of the first color, when it has
been in contact with said substance.
[0012] It is recalled here that the hue of a color corresponds to
the wavelengths (or to the single wavelength in the case of a color
of the rainbow) of the light emitted by the object having that
color. The hue is only one of the components of the color, which
depends on other parameters such as luminosity and saturation.
[0013] Conventional three-part stoppers for straws maintain the
same hue when the straw is in the empty state and the filled state.
The dry or matt aspect of the powder of the stopper when the straw
is in the empty state and the moist or satin aspect of the paste or
gel of the stopper when the straw is an the filled state are linked
to the other parameters of color.
[0014] The indicator component comprised by stopper of the straw
according to the invention makes it possible to know whether the
stopper has been in contact with the substance to preserve in the
straw, by determining whether the indicator component has taken on
the hue of the second color.
[0015] Such determination is particularly simple to perform,
visually by an operator or automatically.
[0016] The implementation of the straw according to the invention
and of the detection of the proper filling thereof is furthermore
feasible in a particularly simple and economical manner, in
particular as set out below.
[0017] According to advantageous features: [0018] said indicator
component emits light of which the spectrum comprises at least one
peak having a crest of predetermined wavelength when it has been in
contact with said substance and does not comprise said peak in the
absence of prior contact with said substance; [0019] said indicator
component emits light of which the spectrum comprises said at least
one peak in response to illumination by light comprising a
predetermined range of wavelengths that is offset relative to the
predetermined wavelength of said crest; [0020] said stopper is
formed by two plugs made from a fibrous substance enclosing a
sealing agent formed by a powder transforming on contact with said
substance into an impermeable paste or gel adhering to the wall of
the tube so that the stopper is liquid-tight, said indicator
component comprising said sealing agent; [0021] said powder
comprises a powder of a salt that is non-fluorophore in the dry
state and fluorophore when it is dissolved in water; [0022] said
salt forms part of the group comprising a fluorescein salt, a
Rhodamine B salt, a Rhodamine 6G salt and a salt of Eriochrome.RTM.
Cyanine R; [0023] said powder comprises between 1/100 and 1/100000
by weight of said salt powder; [0024] said powder is formed by said
salt powder and by powder of material that polymerizes on contact
with water; [0025] said powder of material that polymerizes on
contact with water is alginate; [0026] said indicator component
having a first predetermined color in the absence of prior contact
with said substance and a second predetermined color, having a hue
different from the first color hue, when it has been in contact
with said substance; and/or [0027] said stopper is formed by two
plugs made from a braided fibrous substance enclosing a sealing
agent formed by a powder which, on contact with a liquid,
transforms into an impermeable paste or gel adhering to the wall of
the tube so that the stopper is liquid-tight, at least one of the
two plugs comprising said indicator thread.
[0028] The invention is also directed to a set comprising a straw
as set forth above and a device for checking filling of said straw,
characterized in that said checking device comprises a detection
member for detecting whether said indicator component has taken on
said hue of the second color.
[0029] According to advantageous features: [0030] said indicator
component of the stopper of the straw emits light of which the
spectrum comprises at least one peak having a crest of
predetermined wavelength when the indicator component has been in
contact with said substance and does not comprise said peak in the
absence of prior contact with said substance; and said detection
member comprised by the checking device is configured to detect the
absence from or the presence in the spectrum formed by the light
emitted by said indicator component, of said at least one peak;
[0031] said indicator component of the stopper of the straw emits
light of which the spectrum comprises said at least one peak in
response to illumination by light comprising a predetermined range
of wavelengths that is offset relative to the predetermined
wavelength of said crest; and said checking device further
comprises an illuminating member to illuminate said indicator
component by light comprising said predetermined range of
wavelengths; and/or [0032] the checking device forms part of a
filler machine for straws and comprises a processing unit linked to
a detection member configured to emit a stop signal for the filler
machine if the detection member detects a straw in which the
indicator component has not taken on said hue of the second
color.
[0033] The disclosure of the invention will now be continued with
the description of embodiments, given below given below by way of
non-limiting example, with reference to the attached drawings, in
which:
[0034] FIG. 1 is a diagrammatic view in longitudinal section of a
straw according to the invention, in the empty state;
[0035] FIG. 2 is a view similar to FIG. 1 but showing the straw in
the filled state;
[0036] FIG. 3 is a graph illustrating the spectrum of the light
emitted by a fluorophore agent comprised by the stopper of the plug
when the straw is in the filled state;
[0037] FIG. 4 is a similar view to FIG. 2 but showing the straw in
a machine comprising a device for checking filling of the
straw;
[0038] FIG. 5 is a view similar to FIG. 4 but showing a variant of
the device for checking filling of the straw; and
[0039] FIG. 6 is a similar view to FIG. 3 but for a variant of the
fluorophore agent.
[0040] The straw 10 illustrated in FIG. 1 comprises a tube 11 and a
stopper 12.
[0041] The tube 11 is conventionally made from extruded plastic
material, here transparent, with an inside diameter for example of
1.6 or 2.5 mm and a length of the order of 133 mm.
[0042] The stopper 12 is of the three-part type, i.e. formed by two
plugs 13 and 14 made from a fibrous substance enclosing a sealing
agent 20 formed by a powder (FIG. 1) which, on contact with a
liquid, is capable of transforming into an impermeable paste or gel
15' (FIG. 2) adhering to the wall of the tube 11 so that the
stopper 12 is liquid-tight.
[0043] In the initial state, shown in FIG. 1, the stopper 12 is
disposed in the neighborhood of the end 16 of the tube 11 and it is
provided that in the filled state, the dose of liquid-based
substance which must be preserved in the straw 10 is disposed
between the stopper 12 and the end 17 of the tube 11 that is the
furthest from the stopper 12.
[0044] In order to fill the straw 10, the end 16 is placed in
communication with a vacuum source while the end 17 is placed in
communication with a vessel containing the substance to be
introduced into the straw.
[0045] The air initially contained between the stopper 12 and the
end 17 is sucked through the stopper 12 while the substance 21
(FIG. 2) moves forward in the tube 11 until it encounters the
stopper 12, by the end 18 thereof that is turned towards the end 17
of the tube 11, that is to say the end of the stopper 12 that can
be seen on the right in FIGS. 1 and 2.
[0046] The straw 10 is then in the filled state shown in FIG.
2.
[0047] If necessary, after filling, the straw is welded in the
neighborhood of one or both of its ends 16 and 17 and is placed in
cold storage.
[0048] To empty the straw 10, if necessary after cutting the welded
end portions and thawing, there is inserted into the tube 11 a rod
which comes to bear on the end 19 of the stopper 12 (which end is
situated on the opposite side to the end 18).
[0049] Using this rod, the stopper 12 is made to slide in the
manner of a piston towards the end 17 or the end which corresponds
after cutting the welded portion, which causes the expulsion of the
dose of substance 21 which had been introduced into the straw.
[0050] On comparing FIGS. 1 and 2, it will be noted that the
stopper 12 has a different aspect when the straw 10 is in the empty
state (FIG. 1) and the filled state (FIG. 2).
[0051] When the straw 10 is in the empty state, the sealing agent
20 (powder 15) of the stopper 12 is of a first color and when the
straw 10 is in the filled state, the sealing agent 20 (gel 15') is
of a second color.
[0052] Here, the hue of the first color (empty state) is brownish
white while the hue of the second color (filled state) is greenish
yellow.
[0053] For example, the powder 15, as seen through tube 11, is of
Pantone.RTM. 155U color and the gel 15', as seen through the tube
11, is of Pantone.RTM. 395C color.
[0054] It is recalled here that the hue of a color corresponds to
the wavelengths (or to the single wavelength in the case of a color
of the rainbow) of the light emitted by the object having that
color. The hue is only one of the components of the color, which
depends on other parameters such as luminosity and saturation.
[0055] The change in hue between the powder 15 and the gel 15' is
due to the presence, in the sealing agent 20, of a product changing
hue between the dry state and the state dissolved in water.
[0056] Here, the product changing color is the sodium salt of
fluorescein.
[0057] It will be noted that the fluorescein sodium salt is not
spermicidal and therefore is suitable for contact with animal
semen.
[0058] It is known that the fluorescein sodium salt has the
following formula:
##STR00001##
and that it is identified by the number CAS 518-47-8.
[0059] It is also known that the fluorescein sodium salt is a
fluorophore salt i.e. capable of emitting fluorescent light when it
is dissolved in water; while in the dry state it is a
non-fluorophore salt.
[0060] When the sealing agent 20 of the stopper 12 is in the dry
state (powder 15) the fluorescein sodium salt does not emit
fluorescent light since it is in the dry state. When the sealing
agent 20 of the stopper 12 is in the dampened state (gel 15'), the
fluorescein sodium salt is dissolved in the water contained in the
gel 15' and then emits fluorescent light.
[0061] The change in hue of the sealing agent 20 is due to the
addition of fluorescent light.
[0062] By virtue of the presence of the fluorescein sodium salt,
the sealing agent 20 forms an indicator component indicating
contact between the stopper 12 and the substance 21: the sealing
agent 20 is of a predetermined color in the absence of prior
contact with the substance 21 and a second predetermined color,
having a different hue to the hue of the first color, when the
sealing agent 20 has been in contact with the substance 21.
[0063] It will be observed, as illustrated in FIG. 2, that in the
dampened state of the stopper 12, part of the plug 13 has taken on
the same hue as the gel 15'.
[0064] As a matter of fact, on filling the straw, between the
moment when the substance 21 reached the powder 15 and the moment
when the powder 15 transformed into a liquid-tight gel 15', a small
quantity of powder 15 dissolved by the substance 21 but not yet
gelled was absorbed by the plug 13.
[0065] The indicator component indicating contact with the
substance 21, formed by the sealing agent, is useful for checking
the proper filling of the straw 10, and more precisely the proper
dampening of the stopper 12 by the substance 21.
[0066] It is known that it is very important, for the proper
preservation of the substance 21 contained in the straw 10, that
the stopper 12 be correctly dampened. As a matter of fact, in a
case in which the straw 10 is not welded at its ends, or welded
only at the end 17 furthest from the stopper 12, the
fluid-tightness of the straw 10 is ensured in part by the stopper
12.
[0067] On emptying the straw 10, the correct dampening of the
stopper 12 on filling enables the stopper 12 to play its piston
role without there being leaks between the tube 11 and the stopper
12.
[0068] Checking the proper filling of the straw can be carried out
visually by the operator, simply by verifying that the sealing
agent 20 of the stopper 12 has indeed adopted the hue of the second
predetermined color, i.e. a greenish yellow hue in the present
example.
[0069] The proper filling of the straw 10 can also be checked
automatically, as will be explained below.
[0070] FIG. 3 is a graph illustrating the spectrum of the light
emitted by the fluorescein sodium salt when it is dissolved in
water, i.e. the fluorophore agent contained by the gel 15'. In the
graph of FIG. 3, the wavelengths in nm are along the abscissa axis
and the relative intensity of the emission is along the ordinate
axis.
[0071] It can be seen that the fluorescein sodium salt in the state
dissolved in water emits light of which the spectrum comprises a
peak 22 having a crest 23 the wavelength of which is of the order
of 520 nm, which corresponds to the aforementioned greenish yellow
hue; and that the peak is relatively narrow, which it typical of
light from fluorescence.
[0072] The light emitted by the sealing agent 20 in the dampened
state (gel 15') has a broader spectrum but also comprises a peak 22
having a crest 23.
[0073] In the spectrum, the apex of the peak 22 situated in the
neighborhood of the crest 23 is clearly distinct from the rest of
the spectrum and may thus be relatively easily identified by an
automatic device.
[0074] FIG. 4 shows a machine 25 for filling straws comprising a
device 26 for checking filling of the straws 10 filled by the
machine 25.
[0075] The checking device 26 comprises a member 27 for detecting
whether the indicator component 20 has taken on the hue of the
second color, that is to say the hue of the gel 15'.
[0076] Here, the detection member 27 comprises an electronic
photoreceptor member and a member for electronic analysis of the
information provided by the photoreceptor member to determine
whether the spectrum of the light received by the photoreceptor
member comprises the apex of the peak 22 situated in the
neighborhood of the crest 23.
[0077] In addition to the detection member 27, the checking device
26 comprises a processing unit 28 linked to the detection member 27
and configured to emit a stop signal for the filling machine 25 in
case the detection member 27 detects a straw 10 in which the
indicator component 20 has not taken on the hue of the second
color.
[0078] When the stop signal is emitted, the filling machine 25
stops.
[0079] An operator may then identify the reasons for the improper
filling of the straw and solve the problem which may have
occurred.
[0080] It will be noted that in the dry state, the fluorescein
sodium salt is hydrophilic. It thus dissolves practically
instantaneously in contact with the liquid-based substance 21 which
comprises a high proportion of water. Therefore, the reaction time
of the indicator component indicating contact formed by the sealing
agent 20 is particularly brief.
[0081] By virtue of this high reaction speed, the filling device 26
may be disposed in the machine 25 at a filler station and
practically instantaneously detect a filling defect of the
straw.
[0082] By stopping the filler machine, the number of mis-filled
straws is minimized, which is very important since in general the
substance to preserve in a straw has a high economic value.
[0083] The fast reaction time enables the checking of the filling
to be implemented including in machines with a high operating rate,
capable of filling up to several thousand straws per hour.
[0084] The device for checking filling 26 shown in FIG. 4 is
capable of operating in ambient light.
[0085] In the variant shown in FIG. 5, which is useful in
particular in the situations in which the ambient light is
insufficient, the checking device 26 further comprises a lighting
member 29 for the sealing agent 20.
[0086] It is known that fluorescein sodium salt in the dissolved
state in water is mainly excited by a set of wavelengths situated
in the neighborhood of 495 nm, i.e. in a predetermined range of
wavelengths that is offset relative to the wavelength of the crest
23 (520 nm).
[0087] The lighting member 29 emits light comprising that
predetermined range of wavelengths.
[0088] It is advantageous, so as to avoid the lighting member 29
perturbing the detection member 27, to configure the lighting
member 29, by taking advantage of the offset between the excitation
wavelength and the emission wavelength so as not to emit light or
in any event a minimum of light in the emission range of
wavelengths.
[0089] In the example illustrated of the stopper 12, the powder 15
comprises 1/1000 by weight of fluorescein sodium salt powder in the
dry state.
[0090] A range suitable for the implementation of the invention is
from 1/100 to 1/100000 by weight of fluorescein sodium salt powder
in the dry state.
[0091] Advantageously, the range is from 1/500 to 1/50000, and
still more advantageously from 1/1000 to 1/25000.
[0092] To obtain good homogeneity, the powder 15 is prepared by
successive mixing operations.
[0093] In the illustrated example of the stopper 12, the powder 15
is exclusively formed by fluorescein sodium salt powder in the dry
state and by powder of material that polymerizes on contact with
water.
[0094] Here, the powder of material that polymerizes on contact
with water is alginate.
[0095] In a variant of the sealing agent 20, the fluorescein sodium
salt powder in the dry state is replaced by another product that is
not fluorophore in the dry state and is fluorophore when it is
dissolved in water, which is in the form of a salt in the dry
state.
[0096] This is for example another fluorescein salt, a Rhodamine B
salt, a Rhodamine 6G salt and/or a salt of Eriochrome.RTM. Cyanine
R.
[0097] It is known that Rhodamine B has the following formula:
##STR00002##
and that it is identified by the number CAS 81-88-9.
[0098] It is known that Rhodamine 6G has the following formula:
##STR00003##
and that it is identified by the number CAS 989-38-8.
[0099] It is known that Eriochrome.RTM. Cyanine R has the following
formula;
##STR00004##
and that it is identified by the number CAS 64-18-9.
[0100] FIG. 6 shows in the same way as FIG. 3 the light spectrum
emitted by a mixture of fluorescein, Rhodamine 6G and Rhodamine
B.
[0101] This spectrum comprises a peak 35 having a crest 36 for
Rhodamine 6G and a peak 37 having a crest 38 for Rhodamine B.
[0102] It can be seen that the crest 36 has a wavelength of the
order of 553 nm and that the crest 38 has a wavelength of the order
of 579 nm.
[0103] The fluorophore agent comprised by the sealing agent 20 in
the dampened state (gel 15') may be formed, as illustrated in FIG.
6, by a mixture of fluorescein, Rhodamine 6G and Rhodamine B or by
only one or by two of these products.
[0104] By selecting one or more of these products, the hue of the
sealing agent 20 in the dampened state (gel 15') can be adjusted.
The selection of the hue of the indicator component constituted by
the sealing agent 20 when it has been in contact with the
substance, enables the straw 10 to be recognized, visually or by
analysis of the light spectrum emitted, in particular based on the
location of the crest or crests such as 23, 36 and 38, chosen so as
not to be superposed.
[0105] In other variants, the agent modifying the color of the
indicator component is a colorant, without being a fluorophore.
[0106] In such variants, the light emitted by the indicator
component such as the sealing agent 20 of the stopper 12 does not
comprise a peak having a crest of predetermined wavelength when it
has been in contact with the substance such as animal semen, but a
relatively broad range of wavelengths of the same intensity, which
is less easy to detect automatically.
[0107] The color-modifying agent being a colorant, without being a
fluorophore, is for example methylene blue or .alpha.-zurine.
[0108] Such coloring products, when in the dry state, for example
in the form of a small proportion of the sealing agent 20 in the
dry state (powder 15) do not affect or affect only a very little
the color of the other products forming the indicator component,
for example the alginate powder. On the other hand, when the
indicator component is dampened, the coloring product communicates
its coloration to the rest of the indicator component, for example
the gel 15'.
[0109] In variants not illustrated, the indicator component is
different from the sealing agent 20, for example an indicator
thread having, just as for the sealing agent 20, a first
predetermined color in the absence of prior contact with the
substance 21 and a second predetermined color, having a different
hue from the hue of the first color, when it has been in contact
with the substance 21. Such an indicator thread is associated with
the stopper 12 or with a stopper of different type, for example a
stopper made from a single piece cylinder as described in European
patent application 0 873 726 or in PCT application WO
2010/070533.
[0110] The association of an indicator thread with the stopper 12
is made for example, in the case in which the plug 13 or the plug
14 is a braid made from threads of fibrous substance, by
incorporating the thread in the braid. For example, the indicator
thread is braided with the threads of fibrous substance.
[0111] It is also possible to associate several indicator threads
with the stopper 12.
[0112] For example, the indicator thread or threads are disposed in
the center of the buffer 14 and communicate their coloration to the
rest of the plug 14 when it is dampened.
[0113] In another variant, the indicator component is formed both
by the sealing agent 20 and by the indicator thread associated with
the stopper 12.
[0114] In other variants, the indicator component is different from
the sealing agent and from a thread, for example a ball or a pellet
integrated into the stopper.
[0115] In other variants, the change in color of the indicator
component 15 is brought about on contact with a liquid other than
water, for example a product contained in a diluent or a semen
preservative for animal semen.
[0116] In other variants, the material of the tube such as 11 is
not transparent, but translucent, for example slightly colored; and
the detection takes into account the passage of the light emitted
by the indicator component through the material of the tube such as
11.
[0117] Numerous other variants are possible according to
circumstances, and in this connection it is to be noted that the
invention is not limited to the examples described and shown.
* * * * *