U.S. patent application number 15/143963 was filed with the patent office on 2016-09-01 for micropeptides and use of same for modulating gene expression.
The applicant listed for this patent is CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PAUL SABATIER TOULOUSE III. Invention is credited to Guillaume BECARD, Jerome CAVAILLE, Jean-Philippe COMBIER, Dominique LAURES-SERGUES, Francois PAYRE, Serge PLAZA.
Application Number | 20160251402 15/143963 |
Document ID | / |
Family ID | 52016107 |
Filed Date | 2016-09-01 |
United States Patent
Application |
20160251402 |
Kind Code |
A1 |
COMBIER; Jean-Philippe ; et
al. |
September 1, 2016 |
MICROPEPTIDES AND USE OF SAME FOR MODULATING GENE EXPRESSION
Abstract
Process for detecting and identifying micropeptides (miPEPs)
encoded by a nucleotide sequence contained in the sequence of the
primary transcript of a microRNA and use thereof for modulating
gene expression.
Inventors: |
COMBIER; Jean-Philippe;
(CASTANET TOLOSAN, FR) ; LAURES-SERGUES; Dominique;
(TOULOUSE, FR) ; BECARD; Guillaume; (ODARS,
FR) ; PAYRE; Francois; (POMPERTUZAT, FR) ;
PLAZA; Serge; (RAMONVILLE SAINT AGNE, FR) ; CAVAILLE;
Jerome; (RAMONVILLE SAINT AGNE, FR) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
UNIVERSITE PAUL SABATIER TOULOUSE III
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE |
Toulouse Cedex 9
Paris Cedex 16 |
|
FR
FR |
|
|
Family ID: |
52016107 |
Appl. No.: |
15/143963 |
Filed: |
May 2, 2016 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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PCT/FR2014/052781 |
Oct 31, 2014 |
|
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15143963 |
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Current U.S.
Class: |
504/100 |
Current CPC
Class: |
A61K 48/00 20130101;
A01N 65/20 20130101; A61P 35/00 20180101; A61P 43/00 20180101; C12Q
1/6895 20130101; Y02A 40/146 20180101; A61P 37/02 20180101; A61P
9/00 20180101; A61P 31/04 20180101; C07K 5/10 20130101; C12Q 1/68
20130101; A01N 57/16 20130101; C12N 15/63 20130101; C07K 14/415
20130101; C07K 7/08 20130101; C12N 15/8218 20130101; C07K 7/06
20130101; C12N 15/113 20130101; A01N 37/46 20130101; A01N 65/08
20130101; A01N 65/00 20130101; A01N 65/28 20130101; A61P 3/04
20180101; A61P 25/00 20180101; C12Q 2600/13 20130101; C12Q 2600/178
20130101; A61K 38/00 20130101; C12N 15/8261 20130101; A01N 65/44
20130101; C07K 5/1013 20130101; C12Q 2600/158 20130101; A61P 3/10
20180101 |
International
Class: |
C07K 14/415 20060101
C07K014/415; C07K 7/06 20060101 C07K007/06; C07K 5/103 20060101
C07K005/103; C07K 7/08 20060101 C07K007/08; C12N 15/63 20060101
C12N015/63; A01N 57/16 20060101 A01N057/16; A01N 65/44 20060101
A01N065/44; A01N 65/20 20060101 A01N065/20; A01N 65/28 20060101
A01N065/28; A01N 65/00 20060101 A01N065/00; A01N 37/46 20060101
A01N037/46; C12Q 1/68 20060101 C12Q001/68; A01N 65/08 20060101
A01N065/08 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 31, 2013 |
FR |
13/60727 |
Jun 3, 2014 |
FR |
14/55044 |
Claims
1. Process for detecting and identifying a micropeptide (miPEP)
encoded by a nucleotide sequence contained in the sequence of the
primary transcript of a microRNA, comprising: a) a step of
detecting an open reading frame from 12 to 303 nucleotides in
length contained in the sequence of the primary transcript of said
microRNA, then b) a step of comparison between: the accumulation of
said microRNA in a specified eukaryotic cell expressing said
microRNA, in the presence of a peptide encoded by a nucleotide
sequence that is identical or degenerate relative to that of said
open reading frame, said peptide being present in the cell
independently of transcription of the primary transcript of said
microRNA, and the accumulation of said microRNA in a eukaryotic
cell of the same type as the aforesaid specified eukaryotic cell
expressing said microRNA, in the absence of said peptide, in which
a modulation of the accumulation of said microRNA in the presence
of said peptide relative to the accumulation of said microRNA in
the absence of said peptide indicates the existence of a
micropeptide encoded by said open reading frame.
2. Process for detecting and identifying a miPEP according to claim
1, in which the modulation of the accumulation of said microRNA is
a decrease or an increase in the accumulation of said microRNA, in
particular an increase.
3. Process for detecting and identifying a miPEP according to claim
1, in which the presence of the peptide in the cell results from:
the introduction of a nucleic acid encoding said peptide into the
cell, or the introduction of said peptide into the cell.
4. Process for detecting and identifying a miPEP according to claim
1, in which said open reading frame in step a) is contained in the
5' or 3' portion of said primary transcript of the microRNA,
preferably in the 5' portion.
5. Process for detecting and identifying a miPEP according to claim
1, in which said microRNA is present in a wild-type plant cell or
in a wild-type animal cell, and in which said eukaryotic cell used
in step b) is a plant cell, preferably of a crucifer, of a
leguminous plant or of a plant of the Solanaceae family or is an
animal cell, preferably a human cell or Drosophila cell.
6. Process for detecting and identifying a miPEP according to claim
1, in which said microRNA is of endogenous origin in said
eukaryotic cell used in step b).
7. Process for detecting and identifying a miPEP according to claim
1, in which said microRNA is of exogenous origin in said eukaryotic
cell used in step b), said eukaryotic cell containing a vector
allowing the expression of said microRNA.
8. Process for detecting and identifying a miPEP according to claim
1, in which the accumulation of said microRNA is determined using
quantitative RT-PCR, Northern blot, a DNA or RNA chip.
9. MiPEP encoded by the nucleotide sequence as obtained by the
process according to claim 1.
10. MiPEP from 3 to 100 amino acids, preferably from 4 to 40 amino
acids, encoded by n open reading frame from 12 to 303 nucleotids
contained in a nucleotide sequence contained in the primary
transcript of a microRNA, said miPEP being capable of modulating
the accumulation of said microRNA in a eukaryotic cell, said
nucleotide sequence being contained in the 5' or 3' portion of said
primary transcript of a microRNA, preferably in the 5' portion, the
modulation of the microRNA by said miPEP being evidenced by
observing a variation in the amounts of microRNA quantities between
cells in the presence or in the absence of miPEP.
11. MiPEP according to claim 9, said miPEP being selected from the
group of peptides consisting of: SEQ ID NO: 1 to SEQ ID NO: 104 and
SEQ ID NO: 355, SEQ ID NO: 375 to SEQ ID NO: 386, or SEQ ID NO:
424.
12. Nucleic acid molecule encoding a miPEP as defined according to
claim 9, said nucleic acid molecule being selected in particular
from the group of nucleic acids consisting of: SEQ ID NO: 105 to
SEQ ID NO: 208 and SEQ ID NO: 356, SEQ ID NO: 387 to SEQ ID NO:
399, or SEQ ID NO: 425.
13. A method for modulating the expression of a gene in a specified
eukaryotic cell, comprising exposing said specified eukaryotic cell
at least: a miPEP as defined according to claim 9, a nucleic acid
encoding said miPEP, or a vector containing said nucleic acid, said
specified eukaryotic cell being capable of expressing a microRNA,
the primary transcript of which contains at least one nucleotide
sequence encoding said at least one miPEP and the accumulation of
which is modulated by said at least one miPEP, the expression of
said gene being regulated by said microRNA, said miPEP in
particular being selected from the group of peptides consisting of:
SEQ ID NO: 1 to SEQ ID NO: 104 and SEQ ID NO: 355, SEQ ID NO: 375
to SEQ ID NO: 386, or SEQ ID NO: 424, in particular: SEQ ID NO: 59,
SEQ ID NO: 24, SEQ ID NO: 43, SEQ ID NO: 76, SEQ ID NO: 77, SEQ ID
NO: 14, SEQ ID NO: 63 and SEQ ID NO: 424, said nucleic acid in
particular being selected from the group of nucleic acids
consisting of: SEQ ID NO: 105 to SEQ ID NO: 208 and SEQ ID NO: 356,
SEQ ID NO: 387 to SEQ ID NO: 399, or SEQ ID NO: 425, in particular:
SEQ ID NO: 163, SEQ ID NO: 128, SEQ ID NO: 147, SEQ ID NO: 180, SEQ
ID NO: 181, SEQ ID NO: 118, SEQ ID NO: 425 and SEQ ID NO: 167, said
microRNA being selected in particular from the group of nucleic
acids consisting of: SEQ ID NO: 282 to SEQ ID NO: 354, and SEQ ID
NO: 358, SEQ ID NO: 412 to SEQ ID NO: 423, or SEQ ID NO: 427, in
particular SEQ ID NO: 319, SEQ ID NO: 297, SEQ ID NO: 305, SEQ ID
NO: 331, SEQ ID NO: 291, SEQ ID NO: 322 and SEQ ID NO: 427.
14. Composition comprising at least: a miPEP as defined according
to claim 9, a nucleic acid encoding said miPEP, or a vector
containing said nucleic acid. for use as a human or veterinary
medicament.
15. Process for modulating the expression of a gene regulated by a
microRNA in a eukaryotic plant or animal cell, in particular a
human cell, comprising carrying out a step of accumulation of a
miPEP in said eukaryotic cell, said miPEP having: a size from 3 to
100 amino acids, preferably 4 to 20 amino acids, and a peptide
sequence identical to that encoded by a nucleotide sequence
contained in the primary transcript of a microRNA regulating the
expression of said gene, and being capable of modulating the
accumulation of said microRNA, in which the accumulation of said
miPEP in said eukaryotic cell induces a modulation of the
expression of said gene relative to the expression of said gene
without accumulation of said miPEP, said process not being used for
surgical or therapeutic treatment of the human body or animal body,
nor for modifying the genetic identity of a human being.
16. A method for promoting the growth and/or development of plants,
in particular for modulating the physiological parameters of a
plant, in particular the biomass, foliar surface area, flowering,
fruit size, production and/or selection of plant seeds, in
particular for controlling the parthenocarpy or monoecism of a
plant, or for modifying the physiological parameters of plant
seeds, in particular germination, establishment of the root system
and resistance to water stress, or for preventing or treating plant
diseases,in particular for promoting resistance to infectious
diseases, comprising administering to a plant in need there of a
composition comprising at least: a miPEP as defined according to
claim 9, a nucleic acid encoding said miPEP, or a vector containing
said nucleic acid, as a phytopharmaceutical agent.
17. A method for production and/or selection of plant seeds,
comprising applying a coating or film-coating of said plant seeds a
composition comprising at least: a miPEP as defined according claim
9, a nucleic acid encoding said miPEP, or a vector containing said
nucleic acid, as a phytopharmaceutical agent.
18. A method for eradicating plants or slowing their growth
comprising administering to the plants a composition at least: a
miPEP as defined according to claim 9, a nucleic acid encoding said
miPEP, or a vector containing said nucleic acid, as a
herbicide.
19. A method for eradicating organisms harmful to plants or liable
to be classified as such, in particular as insecticide,
arachnicide, molluscicide, or rodenticide, comprising applying to a
plant or to a support in contact with the plantcomposition
comprising at least: a miPEP as defined according to claim 9, a
nucleic acid encoding said miPEP, or a vector containing said
nucleic acid, as a pesticide.
20. A composition comprising, in combination, a quantity of seeds
of a plant and a quantity of a peptide the sequence of which
comprises or consists of a sequence identical to that of a miPEP
naturally present in said plant, in particular said composition
being formulated so as to form a coated seed.
Description
FIELD OF THE INVENTION
[0001] The present invention relates to micropeptides (peptides
encoded by microRNAs or "miPEPs") and use thereof for modulating
gene expression.
BACKGROUND OF THE INVENTION
[0002] The microRNAs (miRNAs) are small non-coding RNAs, about 21
nucleotides in length after maturation, which control expression of
target genes at the post-transcriptional level, by degrading the
target mRNA or by inhibiting its translation. The miRNAs occur in
plants and animals.
[0003] The target genes are often key genes in developmental
processes. For example they encode transcription factors or
proteins of the proteasome.
[0004] The regulation of expression of the miRNAs is very poorly
understood, but it is known in particular that the latter involves,
like most coding genes, an RNA polymerase II: this enzyme produces
a primary transcript, called "pri-miRNA", which is then matured by
a protein complex in particular containing the Dicer type enzymes.
This maturation leads firstly to the formation of a precursor of
miRNA called "pre-miRNA", having a stem-loop secondary structure
containing the miRNA and its complementary sequence miRNA*. Then
the precursor is matured, which leads to formation of a shorter
double-stranded RNA containing the miRNA and the miRNA*. The miRNA
is then manipulated by the RISC complex, which cleaves the mRNA of
the target gene or inhibits its translation.
[0005] Moreover, it has been shown that the presence of introns in
the primary transcript of the microRNA increases the expression of
the mature microRNA (Schwab et al., EMBO Rep., 14(7): 615-21,
2013). However, owing to experimental difficulties, the primary
transcripts of microRNAs, or pri-miRNAs, have received very little
study.
[0006] About 50% of eukaryotic genes have small open reading frames
within their 5'UTR region (5' UnTranslated Region) upstream of the
coding sequence. These small open reading frames (or "uORFs" for
upstream ORFs) may perform a role of translation regulator, mainly
in cis, by modulating the fixation and the rate of the ribosomes on
the mRNA, but also in trans by an as yet unknown mechanism, by
means of peptides encoded by said uORFs (Combier et al., Gene Dev,
22: 1549-1559, 2008). By definition, the uORFS are present upstream
of coding genes.
[0007] Recently, small ORFs have also been discovered in long
intergenic non-coding RNAs (lincRNAs), the putative function of
which, if it exists, is not known (Ingolia et al., Cell, 147(4):
789-802, 2011; Guttman & Rinn, Nature, 482(7385): 339-46,
2012).
[0008] However, no example has yet been reported concerning the
existence of ORFs encoding peptides within non-coding microRNAs.
Until now, the microRNAs, and by extension their primary
transcript, have always been regarded, based on their particular
mode of action, as non-coding regulatory RNAs that do not produce
any peptide.
SUMMARY OF THE INVENTION
[0009] One of the aspects of the invention is to propose peptides
capable of modulating the expression of microRNAs.
[0010] Another aspect of the invention is to propose a means for
modulating the expression of one or more target genes of a
microRNA.
[0011] The present invention offers the advantage of allowing
easier and more effective control of the expression of genes
targeted by the microRNAs, through a means other than the
microRNA.
[0012] The invention thus relates to a process for detecting and
identifying a micropeptide (miPEP) encoded by a nucleotide sequence
contained in the sequence of the primary transcript of a
microRNA,
[0013] comprising: [0014] a) a step of detecting an open reading
frame from 12 to 303 nucleotides in length contained in the
sequence of the primary transcript of said microRNA, then [0015] b)
a step of comparison between: [0016] the accumulation of said
microRNA in a specified eukaryotic cell expressing said microRNA,
[0017] in the presence of a peptide encoded by a nucleotide
sequence that is identical or degenerate relative to that of said
open reading frame, said peptide being present in the cell
independently of transcription of the primary transcript of said
microRNA, and [0018] the accumulation of said microRNA in a
eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell expressing said microRNA, in the absence of said
peptide,
[0019] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
[0020] The invention relates in particular to a process for
detecting and identifying a micropeptide (miPEP) encoded by a
nucleotide sequence contained in the sequence of the primary
transcript of a microRNA,
[0021] comprising: [0022] a) a step of detecting an open reading
frame from 15 to 303 nucleotides in length contained in the
sequence of the primary transcript of said microRNA, then [0023] b)
a step of comparison between: [0024] the accumulation of said
microRNA in a specified eukaryotic cell expressing said microRNA,
[0025] in the presence of a peptide encoded by a nucleotide
sequence that is identical or degenerate relative to that of said
open reading frame, said peptide being present in the cell
independently of transcription of the primary transcript of said
microRNA, and [0026] the accumulation of said microRNA in a
eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell expressing said microRNA, in the absence of said
peptide,
[0027] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
[0028] The invention relates in particular to a process for
detecting and identifying a micropeptide (miPEP) encoded by a
nucleotide sequence contained in the sequence of the primary
transcript of a microRNA,
[0029] comprising: [0030] a) a step of detecting an open reading
frame from 15 to 303 nucleotides in length, or of 12 nucleotides in
length, contained in the sequence of the primary transcript of said
microRNA, then [0031] b) a step of comparison between: [0032] the
accumulation of said microRNA in a specified eukaryotic cell
expressing said microRNA, [0033] in the presence of a peptide
encoded by a nucleotide sequence that is identical or degenerate
relative to that of said open reading frame, said peptide being
present in the cell independently of transcription of the primary
transcript of said microRNA, and [0034] the accumulation of said
microRNA in a eukaryotic cell of the same type as the aforesaid
specified eukaryotic cell expressing said microRNA, in the absence
of said peptide,
[0035] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
[0036] The invention relates in particular to a process for
detecting and identifying a micropeptide (miPEP) encoded by a
nucleotide sequence contained in the sequence of the primary
transcript of a microRNA,
[0037] comprising: [0038] a) a step of detecting an open reading
frame of 12 nucleotides in length contained in the sequence of the
primary transcript of said microRNA, then [0039] b) a step of
comparison between: [0040] the accumulation of said microRNA in a
specified eukaryotic cell expressing said microRNA, [0041] in the
presence of a peptide encoded by a nucleotide sequence that is
identical or degenerate relative to that of said open reading
frame, said peptide being present in the cell independently of
transcription of the primary transcript of said microRNA, and
[0042] the accumulation of said microRNA in a eukaryotic cell of
the same type as the aforesaid specified eukaryotic cell expressing
said microRNA, [0043] in the absence of said peptide,
[0044] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
[0045] In a first step, the process for detecting and identifying a
micropeptide therefore consists of detecting, on the primary
transcript of a microRNA, the existence of an open reading frame
potentially encoding a peptide.
[0046] For its part, the second step makes it possible to
characterize said peptide, i.e. to determine whether said peptide
corresponds to a peptide really produced in the cell, by searching
for an effect of said peptide on the accumulation of said
microRNA.
[0047] In order to demonstrate an effect of the peptide on the
accumulation of the microRNA, a large quantity of peptide is
introduced into a first cell expressing said microRNA. The
accumulation of the microRNA in this first cell is then measured
and compared with the accumulation of the microRNA in a second cell
identical to the first, but not containing said peptide.
[0048] Observation of a variation of the quantities of microRNA
between the cells in the presence and in the absence of the peptide
thus indicates (i) that there is a peptide encoded on the primary
transcript of said microRNA, (ii) that the sequence of this peptide
is encoded by the open reading frame identified on the primary
transcript of said microRNA, and (iii) that said peptide has an
effect on the accumulation of said microRNA.
[0049] The invention is therefore based on the unexpected double
observation made by the inventors that, on the one hand, there are
open reading frames that are able to encode micropeptides present
on the primary transcripts of microRNAs, and on the other hand that
said micropeptides are capable of modulating the accumulation of
said microRNAs.
[0050] In particular, the invention relates to a process for
detecting and identifying a micropeptide (miPEP) encoded by a
nucleotide sequence contained in the sequence of the primary
transcript of a microRNA,
[0051] comprising: [0052] a) a step of detecting an open reading
frame from 15 to 303 nucleotides in length contained in the
sequence of the primary transcript of said microRNA, then [0053] b)
a step of comparison between: [0054] the accumulation of said
microRNA in a specified eukaryotic cell expressing the primary
transcript of said microRNA, [0055] in the presence of a peptide
encoded by a nucleotide sequence that is identical or degenerate
relative to that of said open reading frame, said peptide being
present in the cell independently of transcription of the primary
transcript of said microRNA, and [0056] the accumulation of said
microRNA in a eukaryotic cell of the same type as the aforesaid
specified eukaryotic cell expressing the primary transcript of said
microRNA, [0057] in the absence of said peptide,
[0058] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
[0059] In particular, the invention relates to a process for
detecting and identifying a micropeptide (miPEP) encoded by a
nucleotide sequence contained in the sequence of the primary
transcript of a microRNA,
[0060] comprising: [0061] a) a step of detecting an open reading
frame from 15 to 303 nucleotides in length, or of 12 nucleotides in
length, contained in the sequence of the primary transcript of said
microRNA, then [0062] b) a step of comparison between: [0063] the
accumulation of said microRNA in a specified eukaryotic cell
expressing the primary transcript of said microRNA, [0064] in the
presence of a peptide encoded by a nucleotide sequence that is
identical or degenerate relative to that of said open reading
frame, said peptide being present in the cell independently of
transcription of the primary transcript of said microRNA, and
[0065] the accumulation of said microRNA in a eukaryotic cell of
the same type as the aforesaid specified eukaryotic cell expressing
the primary transcript of said microRNA, [0066] in the absence of
said peptide,
[0067] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
[0068] In particular, the invention relates to a process for
detecting and identifying a micropeptide (miPEP) encoded by a
nucleotide sequence contained in the sequence of the primary
transcript of a microRNA,
[0069] comprising: [0070] a) a step of detecting an open reading
frame of 12 nucleotides in length contained in the sequence of the
primary transcript of said microRNA, then [0071] b) a step of
comparison between: [0072] the accumulation of said microRNA in a
specified eukaryotic cell expressing the primary transcript of said
microRNA, [0073] in the presence of a peptide encoded by a
nucleotide sequence that is identical or degenerate relative to
that of said open reading frame, said peptide being present in the
cell independently of transcription of the primary transcript of
said microRNA, and [0074] the accumulation of said microRNA in a
eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell expressing the primary transcript of said microRNA,
[0075] in the absence of said peptide,
[0076] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a micropeptide encoded by said open reading frame.
BRIEF DESCRIPTION OF THE DRAWINGS
[0077] FIG. 1. Effects of overexpression of MtmiR171b (miR171b
identified in Medicago truncatula) on the expression of the HAM1
and HAM2 genes (A) or on the number of lateral roots (B) in M.
truncatula.
[0078] (A) The y-axis indicates the relative expression of
MtmiR171b (left-hand columns), of HAM1 (middle columns) or of HAM2
(right-hand columns) in a control plant (white columns) or in a
plant in which MtmiR171b is overexpressed (black columns). The
error bar corresponds to the standard error of the mean (number of
individuals=10). The overexpression of MtmiR171b induces a decrease
in the expression of the HAM1 and HAM2 genes.
[0079] (B) The y-axis indicates the mean number of lateral roots
observed in a control plant (white column) or in a plant in which
MtmiR171b is overexpressed (black column). The error bar
corresponds to the standard error of the mean (number of
individuals=100). The overexpression of MtmiR171b leads to a
reduction in the number of lateral roots.
[0080] FIG. 2. Effects of overexpression of MtmiPEP171b1 on the
expression of MtmiR171b and of the HAM1 and HAM2 genes (A) or on
the number of lateral roots (B) in M. truncatula.
[0081] (A) The y-axis indicates the relative expression of
MtmiPEP171b1 (graph on left), miR171b (graph on right, left-hand
columns), of HAM1 (accession No. MtGI9-TC114268) (graph on right,
middle columns) or of HAM2 (accession No. MtGI9-TC120850) (graph on
right, right-hand columns) in a control plant (white columns) or in
a plant in which MtmiPEP171b1 is overexpressed (black columns). The
error bar corresponds to the standard error of the mean (number of
individuals=10). The overexpression of MtmiPEP171b1 induces an
increase in the accumulation of MtmiR171b, as well as a decrease in
the expression of the HAM1 and HAM2 genes.
[0082] (B) The y-axis indicates the mean number of lateral roots
observed in a control plant (white column) or in a plant in which
MtmiPEP171b1 is overexpressed (black column). The error bar
corresponds to the standard error of the mean (number of
individuals=100). The overexpression of MtmiPEP171b1 leads to a
reduction in the number of lateral roots.
[0083] FIG. 3. Effects of MtmiPEP171b1 on the expression of
MtmiR171b and the HAM1 and HAM2 genes (A) and on the number of
lateral roots (B) in M. truncatula.
[0084] (A) The y-axis indicates the relative expression of
MtmiR171b (left-hand columns), of HAM1 (middle columns) or of HAM2
(right-hand columns) in a control plant (white columns) or in a
plant treated by watering once daily for 5 days with MtmiPEP171b1
at 0.01 .mu.M (light grey columns), 0.1 .mu.M (dark grey columns)
or 1 .mu.M (black columns). The error bar corresponds to the
standard error of the mean (number of individuals=10). Application
of MtmiPEP171b1 at different concentrations induces an increase in
the accumulation of MtmiR171b, as well as a decrease in the
expression of the HAM1 and HAM2 genes.
[0085] (B) The y-axis indicates the mean number of lateral roots
observed in a control plant (white column) or in a plant treated by
watering with MtmiPEP171b1 at 0.1 .mu.M once daily for 5 days
(black column). The error bar corresponds to the standard error of
the mean (number of individuals=100). Application of MtmiPEP171b1
at 0.1 .mu.M leads to a reduction in the number of lateral
roots.
[0086] (C) The y-axis indicates the relative expression of
MtmiR171b (left-hand columns), of HAM1 (middle columns) or of HAM2
(right-hand columns) in a control plant (white columns) or in a
plant treated by watering once daily for 5 days with MtmiPEP171b1
at 0.01 .mu.M (grey columns), 0.1 .mu.M (dark grey columns) or 1
.mu.M (black columns) or with 0.01 .mu.M of a scramble peptide
(LIVSHLYSEKFDCMRKILRI, SEQ ID NO: 428) (light grey columns) the
amino acid composition of which is identical to miPEP171b but the
sequence of which is different. The error bar corresponds to the
standard error of the mean (number of individuals=10).
[0087] FIG. 4. Effects of MtmiPEP171b1 on the expression of
pre-MtmiR171b (A) and of MtmiR171b (B) in M. truncatula.
[0088] The y-axis indicates the relative expression of the
precursors of the different forms of the microRNA in control plants
(left-hand column) or in plants treated by watering once daily for
5 days with MtmiPEP171b1 at 0.01 .mu.M, 0.1 .mu.M or 1 .mu.M
(right-hand columns). The error bar corresponds to the standard
error of the mean (number of individuals=200). Application of
MtmiPEP171b1 at different concentrations leads to an increase in
the accumulation of pre-MtmiR171b (A) and of MtmiR171b (B).
[0089] FIG. 5. Effects of overexpression of MtmiPEP171b1 (A) and
effects of MtmiPEP171b1 (B) on the expression of different
precursors of microRNAs in M. truncatula.
[0090] The y-axis indicates the ratio of the expression of the
precursors of microRNAs in plants overexpressing MtmiPEP171b1 to
the expression of these same precursors in control roots (A), or
the ratio of the expression of the precursors of microRNAs in
plants treated with MtmiPEP171b1 (0.1 .mu.M) to the expression of
these same precursors in control roots (B). The different
precursors of microRNAs tested are indicated from left to right on
the x-axis, namely pre-MtmiR171b (SEQ ID NO: 246), pre-MtmiR169
(SEQ ID NO: 359), pre-MtmiR169a (SEQ ID NO: 360), pre-MtmiR171a
(SEQ ID NO: 361), pre-MtmiR171h (SEQ ID NO: 362), pre-MtmiR393a
(SEQ ID NO: 363), pre-MtmiR393b (SEQ ID NO: 364), pre-MtmiR396a
(SEQ ID NO: 365) and pre-MtmiR396b (SEQ ID NO: 366). The error bar
corresponds to the standard error of the mean (number of
individuals=10). It is noted that MtmiPEP171b1 only leads to an
effect on the accumulation of MtmiR171b and not on the other
miRNAs.
[0091] FIG. 6. Effects of translation of MtmiPEP171b1 on the
expression of MtmiR171b demonstrated in the model plant Nicotiana
benthamiana. The y-axis indicates the relative expression of
MtmiR171b in tobacco plants transformed in order to express
pri-MtmiR171b (white column) or a mutated pri-MtmiR171b in which
the codon ATG has been replaced with ATT (black column). The
mutated pri-MtmiR171b is therefore incapable of producing
MtmiPEP171b1. The error bar corresponds to the standard error of
the mean (number of individuals=30). It is noted that the absence
of translation of MtmiPEP171b1 leads to a marked decrease in the
accumulation of miR171b.
[0092] FIG. 7. Effects of overexpression of MtmiPEP171b1 on the
expression of pre-MtmiR171b demonstrated in the model plant
Nicotiana benthamiana.
[0093] The y-axis indicates the relative expression of
pre-MtmiR171b in tobacco plants that have been transformed in order
to express MtmiR171b (left-hand column), MtmiR171b and MtmiPEP171b1
(middle column), or MtmiR171b and a mutated version of MtmiORF171b
in which the start codon ATG has been replaced with ATT (right-hand
column). The error bar corresponds to the standard error of the
mean (number of individuals=30). It is noted that the expression of
MtmiPEP171b1 increases the expression of MtmiR171b, and this effect
is dependent on the translation of MtmiORF171b to MtmiPEP171b1.
[0094] FIG. 8. Effects of MtmiPEP171b1 on the expression of
pre-MtmiR171b demonstrated in the model plant Nicotiana
benthamiana.
[0095] The y-axis indicates the relative expression of MtmiR171b in
tobacco plants transformed in order to express MtmiR171b onto which
MtmiPEP171b1 has been sprayed (0.1 .mu.M) twice, 12 h and then 30
min before sampling (right-hand column) or not (left-hand column).
The error bar corresponds to the standard error of the mean (number
of individuals=6). The peptide MtmiPEP171b1 applied by spraying
induces an increase in the accumulation of MtmiR171b.
[0096] FIG. 9. Effects of MtmiPEP171b1 on the expression of
pri-miR171b (A), pre-MtmiR171b (B) and MtmiR171b (C) demonstrated
in the model plant Nicotiana benthamiana.
[0097] The y-axis indicates the relative expression of the
precursors of the different forms of the microRNA in tobacco plants
modified in order to express MtmiR171b (left-hand column) or
modified in order to express MtmiR171b and overexpress MtmiPEP171b1
(right-hand columns, FIG. 9A) or treated with 0.1 .mu.M of
miPEP171b1 (FIGS. 9B and C). The error bar corresponds to the
standard error of the mean (number of individuals=30). The
overexpression of MtmiPEP171b1 or application of miPEP171b1
increases the accumulation of pri-MtmiR171b (A), pre-MtmiR171b (B)
and MtmiR171b (C).
[0098] FIG. 10. Localization of MtmiPEP171b1 in tobacco leaf cells
that have been modified in order to express MtmiPEP171b1.
[0099] The photographs show tobacco leaf cells modified in order to
express the protein GFP alone (left panel) or the protein GFP fused
to MtmiPEP171b1 (right panel). These observations indicate that
MtmiPEP171b1 is localized in small nuclear bodies.
[0100] FIG. 11. Effects of the expression of AtmiPEP165a
(identified in Arabidopsis thaliana) on the expression of AtmiR165a
(A), and of the expression of AtmiPEP319a2 (identified in
Arabidopsis thaliana) on AtmiR319a (B), demonstrated in the model
plant of tobacco.
[0101] (A) The y-axis indicates the relative expression of
AtmiR165a in tobacco plants modified in order to express AtmiR165a
(left-hand column) or to express AtmiR165a and AtmiPEP165a
(right-hand column).
[0102] (B) The y-axis indicates the relative expression of
AtmiR319a in tobacco plants modified in order to express AtmiR319a
(left-hand column) or in order to express AtmiR319a and AtmiPEP319a
(right-hand column).
[0103] The error bar corresponds to the standard error of the mean
(number of individuals=30). In both cases, it is noted that the
expression of miORF, and therefore the production of miPEP, leads
to an increase in the accumulation of pre-miRNA.
[0104] FIG. 12. Effects of treatment with AtmiPEP165a on root
growth in Arabidopsis thaliana.
[0105] The photograph shows two plants of the same age: a control
plant (plant on the left) and a plant treated with AtmiPEP165a
(plant on the right). The treatment with AtmiPEP165a leads to a
phenotype with greatly accelerated root growth in Arabidopsis
thaliana. The graph shows the expression of pre-miR165 in response
to treatment with increasing doses of AtmiPEP165a.
[0106] FIG. 13. Conservation of the sequence of miPEP8 identified
in Drosophila.
[0107] The sequences of miPEP8 (SEQ ID NO: 104) were deduced from
the sequences of miORF8 (SEQ ID NO: 208) of 12 different Drosophila
species and were aligned. A histogram shows the conservation of
each amino acid between the sequences of miORF8 in the 12 species
analysed.
[0108] FIG. 14. Evolution of the mass (kDa) and isoelectric point
(pI) of miPEP8 in the Drosophila species.
[0109] The y-axis on the left indicates the size of the miPEP8 (in
kD). The y-axis on the right indicates the isoelectric point of the
miPEP. The x-axis indicates the origin of the miPEP, i.e. the
Drosophila species. It is noted that despite a significant change
in their size (by more than a factor of 3), the charge of the
miPEPs is still very basic (>9.8) in the 12 species studied.
[0110] FIG. 15. Effect of the addition of sequences on the function
of miPEP.
[0111] The tobacco leaves were transformed in order to overexpress
miPEP171b. These graphs show that the addition of sequences (tag
His, HA or GFP) does not alter the function of miPEP. The y-axis
indicates the relative expression of pre-MtmiR171b in tobacco
plants that have been transformed in order to express MtmiR171b
(left-hand column), MtmiR171b and MtmiPEP171b1 with or without
addition of protein tags (right-hand columns). The error bar
corresponds to the standard error of the mean (number of
individuals=6). It is noted that the expression of MtmiPEP171b1
increases the expression of MtmiR171b, and this effect is
independent of the presence of tags.
[0112] FIG. 16. Expression of MtmiPEP171b1 in the root system of
Medicago truncatula.
[0113] The roots of Medicago truncatula were transformed in order
to express fusions between GUS protein (in blue) and the promoter
of miR17b (A, E), the ATG of miPEP171b1 (B, F), whole miPEP171b1
(C, G) or ATG2 (second ATG located on the precursor, after miPEP)
(D, H). It is clear that there is expression of miRNA in the root
tips (A) as well as the lateral roots (E). The transcriptional (B,
F) and translational (C, G) fusions show an expression of miPEP171b
in the same tissues, whereas the next ATG is not active (D, H).
[0114] FIG. 17. Expression of DmmiPEP8 in cells of Drosophila
melanogaster
[0115] The cells of Drosphila melanogaster were transfected in
order to overexpress DmmiPEP8 (OE miPEP8) or miPEP8 of which the
translation start codons have been mutated (OE miPEP8 mut). The
y-axis indicates the relative expression of Pre-miR8. The error bar
corresponds to the standard error of the mean (number of
independent experiments=6). It is noted that the expression of
DmmiPEP8 increases the expression of DmmiR8, and this effect is
linked to the translation of the mRNA.
[0116] FIG. 18. Impact of DmmiPEP8 on accumulation of DmmiR8 in
cells of D. melanogaster
[0117] The cells of Drosphila melanogaster were transfected in
order to overexpress wild-type DmmiR8 (OE miR8) or DmmiR8 the
translation start codons of which have been mutated (OE miR8 miPEP8
mut). The y-axis indicates the relative expression of Pre-miR8. The
error bar corresponds to the standard error of the mean (number of
independent experiments=2). It is noted that the presence of
DmmiPEP8 increases the expression of DmmiR8.
[0118] FIG. 19. Impact of HsmiPEP155 on accumulation of HsmiR155 in
cells of Homo sapiens
[0119] HeLa cells of Homo sapiens had been transfected in order to
overexpress HsmiPEP155 (OE miPEP155). The y-axis indicates the
relative expression of Pre-miR155. The error bar corresponds to the
standard error of the mean (number of independent experiments=2).
It is noted that the expression of HsmiPEP155 increases the
expression of HsmiR155.
[0120] FIG. 20. Effects of translation of MtmiPEP171b1 on the
expression of MtmiR171b demonstrated in the model plant Nicotiana
benthamiana.
[0121] The y-axis indicates the relative expression of MtmiR171b in
tobacco plants transformed in order to express pri-miR171b
(left-hand column), a pri-miR171b in which the miORF171b has been
deleted (middle column) or a mutated pri-miR171b in which the codon
ATG has been replaced with ATT (right-hand column). The mutated
pri-miR171b is therefore incapable of producing miPEP171b1. The
error bar corresponds to the standard error of the mean (number of
individuals=30). It is noted that the absence of translation of
miPEP171b1 leads to a marked decrease in the accumulation of
miR171b.
[0122] FIG. 21. Effects of overexpression of MtmiPEP171b1 on the
expression of MtmiR171b demonstrated in the model plant Nicotiana
benthamiana.
[0123] The y-axis indicates the relative expression of MtmiR171b in
tobacco plants that had been transformed with a vector allowing the
expression of miPEP171b and either a second empty vector (white
column), or a vector allowing the expression of mtmiPEP171b (left
black column), or a vector in which the codon ATG of the ORF
encoding mtmiPEP171b has been replaced with ATT (middle black
column), or a vector in which the nucleotide sequence of the ORF
has been mutated without modifying the amino acid sequence of the
translated peptide (miPEP encoded by a degenerate ORF) (right black
column). The error bar corresponds to the standard error of the
mean (number of individuals=30). It is noted that the expression of
MtmiPEP171b1 increases the expression of MtmiR171b, and this effect
is dependent on the translation of MtmiORF171b to MtmiPEP171b1.
[0124] FIG. 22. Effects of AtmiPEP165a on accumulation of AtmiR165a
and of its target genes (PHAVOLUTA: PHV, PHABOL USA: PHB and
REVOLUTA: REV).
[0125] The y-axis indicates the relative expression of AtmiR165a,
PHV, PHB and REV in roots of Arabidopsis thaliana treated with
water (control) or different concentrations of AtmiPEP165a (0.01
.mu.M, 0.1 .mu.M, 1 .mu.M or 10 .mu.M). The error bar corresponds
to the standard error of the mean (number of individuals=10).
[0126] The treatment of plants with higher and higher
concentrations of AtmiPEP165a demonstrates a dose-dependent effect
of the accumulation of AtmiR165a and the negative regulation of its
target genes as a function of the quantity of AtmiPEP165a.
[0127] FIG. 23. Effects of treatment with AtmiPEP164a on the
expression of AtmiR164a in A. thaliana.
[0128] The photographs show the results of a Northern blot analysis
of the accumulation of AtmiR164a in roots treated with water
(control, photograph on left) or with 0.1 .mu.M of a synthetic
peptide, having a sequence identical to that of AtmiPEP164a,
dissolved in water (0.1 .mu.M miPEP164a). The RNA U6 is used as
loading control making it possible to quantify the quantity of
AtmiR164a.
[0129] This experiment was repeated 4 times independently and led
to similar results.
[0130] Treatment of shoots of A. thaliana with 0.1 .mu.M of
miPEP164a leads to an increase in the accumulation of miR164a.
[0131] FIG. 24. Effects of treatment with AtmiPEP164a on the growth
of Arabidopsis thaliana.
[0132] The photographs show two plants (top views and side views)
after 3 weeks of growth: a control plant watered with water (A),
and a plant watered with a composition of 0.1 .mu.M of synthetic
peptide corresponding to AtmiPEP164a (B). Watering plants of
Arabidopsis thaliana with AtmiPEP164a increases plant growth
significantly.
[0133] FIG. 25. Effects of treatment with AtmiPEP165a on the
expression of AtmiR165a in A. thaliana.
[0134] The photographs show the results of a Northern blot analysis
of the accumulation of AtmiR165a in roots treated with water
(control, photograph on left) or with 0.1 .mu.M of a synthetic
peptide, having a sequence identical to that of AtmiPEP165a,
dissolved in water (0.1 .mu.M miPEP165a). The RNA U6 is used as
loading control making it possible to quantify the quantity of
AtmiR165a.
[0135] This experiment was repeated 4 times independently and led
to similar results.
[0136] Treatment of A. thaliana shoots with 0.1 .mu.M of miPEP165a
leads to an increase in the accumulation of miR165a.
[0137] FIG. 26. Effects of overexpression of AtmiPEP319a1 on the
expression of AtmiR319a in A. thaliana.
[0138] The y-axis indicates the relative expression of AtmiR319a in
a control plant (left-hand column) or in a plant in which
AtmiPEP319a1 is overexpressed (right-hand column). The error bar
corresponds to the standard error of the mean (number of
individuals=10). The overexpression of AtmiPEP319a1 induces an
increase in the accumulation of AtmiR319a.
[0139] FIG. 27. Effects of treatment with AtmiPEP319a on the growth
of Arabidopsis thaliana.
[0140] The photographs show two plants (top views and side views)
after 3 weeks of growth: a control plant watered with water (A),
and a plant watered with a composition of 0.1 .mu.M of synthetic
peptide corresponding to AtmiPEP319a1 (B). Watering of the plants
of Arabidopsis thaliana with AtmiPEP319a1 increases plant growth
significantly.
[0141] FIG. 28. Immunolocalization.
[0142] The roots of Medicago truncatula were transformed in order
to express fusions between the GUS protein (in blue) and the ATG of
miPEP171b (Pro.sub.miR171b-ATG1:GUS) or ATG2 (second ATG located on
the precursor, after miPEP) (Pro.sub.miR171b-ATG2:GUS). Labelling
was also carried out with an anti-miPEP171b antibody (miPEP171b).
Immunolocalization of miPEP171b in the roots of M. truncatula
reveals the presence of miPEP171b in the lateral root initiation
sites, showing a co-localization between the microRNA and the
corresponding miPEP.
[0143] FIG. 29. Effects of the overexpression of AtmiPEP160b1 on
the expression of AtmiR160b in A. thaliana.
[0144] The y-axis indicates the relative expression of AtmiR160b in
a control plant (left-hand column) or in a plant in which
AtmiPEP160b1 is overexpressed (right-hand column). The error bar
corresponds to the standard error of the mean (number of
individuals=10). The overexpression of AtmiPEP160b1 induces an
increase in the relative expression of AtmiR160b.
[0145] FIG. 30. Effects of AtmiPEP164a1 on the expression of
AtmiR164a.
[0146] The y-axis indicates the relative expression of AtmiR164a in
a control plant (left-hand column) or in a plant watered once a day
with AtmiPEP164a1 at 0.1 .mu.M (right-hand column). The error bar
corresponds to the standard error of the mean (number of
individuals=10).
[0147] The addition of AtmiPEP164a1 induces induces an increase in
the relative expression of AtmiR164a.
[0148] FIG. 31. Effects of the overexpression of AtmiPEP319a1 on
the expression of AtmiR319a in A. thaliana.
[0149] The y-axis indicates the relative expression of AtmiR319a in
a control plant (left-hand column) or in a plant in which
AtmiPEP319a1 is overexpressed (right-hand column). The error bar
corresponds to the standard error of the mean (number of
individuals=10).
[0150] The overexpression of AtmiPEP319a1 induces an increase in
the relative expression of AtmiR319a.
[0151] FIG. 32. Effects of MtmiPEP169d on the expression of
MtmiR169d in M. truncatula.
[0152] The y-axis indicates the relative expression of MtmiR169d in
a control plant (left-hand column) or in a plant watered once a day
with MtmiPEP169d at 0.1 .mu.M (right-hand column).
[0153] The error bar corresponds to the standard error of the mean
(number of individuals=10).
[0154] The addition of MtmiPEP169d induces an increase in the
relative expression of MtmiR169d.
[0155] FIG. 33. Effects of the overexpression of MtmiPEP171e on the
expression of MtmiR171e in M. truncatula.
[0156] The y-axis indicates the relative expression of MtmiR171e in
a control plant (left-hand column) or in a plant in which
MtmiPEP171e is overexpressed (right-hand column). The error bar
corresponds to the standard error of the mean (number of
individuals=10).
[0157] The overexpression of MtmiPEP171e induces an increase in the
relative expression of MtmiR171e.
[0158] FIG. 34. Localization of the MtmiPEP171b1 in a wildtype
plant M. truncatula.
[0159] Various amounts of synthetic peptides MtmiPEP171b1 (1, 0.5,
or 0.1 nmol) and a total root extract from M. truncatula have been
analysed by immunoblotting with an antibody specific for
MtmiPEP171b1.
[0160] MtmiPEP171b1 is naturally produced in the roots of M.
truncatula.
[0161] FIG. 35. Presence of AtmiPEP165a in a wildtype plant A.
thaliana.
[0162] The upper picture corresponds to the western blotting
analysis of the amount of AtmiPEP165a in a wildtype seeding plant
of A. thaliana Col-0 (left-hand) and a seeding plant of A. thaliana
overexpressing AtmiPEP165a (right-hand). The lower picture
corresponds to the western blotting analysis of the amount of a
control protein (tubulin) in a wildtype seeding plant of A.
thaliana Col-0 (left-hand) and a seeding plant of A. thaliana
overexpressing AtmiPEP165a (right-hand).AtmiPEP165a is naturally
produced in A. thaliana and is present in larger amounts in plants
overexpressing AtmiPEP165a.
[0163] FIG. 36. Action of AtmiPEP165a in A. thaliana.
[0164] (A) The y-axis indicates the relative expression of
pri-miR165a in control roots (left-hand column) or watered once a
day with AtmiPEP165a at 10 .mu.M (right-hand column). The error bar
corresponds to the standard error of the mean (number of
individuals=10).
[0165] The addition of AtmiPEP165a induces an increase in the
relative expression of AtmiR165a.
[0166] (B) The y-axis indicates the relative expression of
pri-miR165a, in the presence of cordycepin (inhitor of the RNA
synthesis), in control roots (grey curve) or watered once a day
with with AtmiPEP165a at 10 .mu.M (black curve). The x-axis
indicates the duration of the treatment with cordycepin.
[0167] The addition of AtmiPEP165a does not induce a better
stability of pri-miR165a.
[0168] (C) The y-axis indicates the relative expression of
pri-miR165a, in wildtype plants (Col-0) or in plants mutated with a
low allele of the second large subunit of RNA polymerase II
(nrpb2-3). The x-axis indicates if the plants have been watered
with water or with AtmiPEP165a at 10 at 10 .mu.M.
[0169] Mutated plants, unlike wildtype plants, are not capable of
accumulating AtmiR165a in response to AtmiPEP165a.
[0170] FIG. 37. Effects of the pri-miR171b on the number of lateral
roots in M. truncatula.
[0171] The y-axis indicates the mean number of lateral roots
observed in a control plant (white column) or in a plant in which
the pri-miR171b is overexpressed (black column). The error bar
corresponds to the standard error of the mean (number of
individuals=100). The overexpression of pri-miR171b induces a
decrease in the number of lateral roots.
[0172] FIG. 38. Translatability of DmmiPEP8 in Drosophila
melanogaster cells
[0173] Some Drosophila melanogaster cells have been transfected to
overexpress DmmiPEP8 (miPEP8::GFP) or DmmiPEPmt (of which start
codons have been mutated (miPEP8 mt::GFP). As a transfection
control, a plasmid producing moesin-RFP protein (lower picture) has
been co-transfected with the plasmids encoding DmmiPEP8 (left-hand
picture) and DmmiPEP8 mt (right-hand picture). It is observed that
the start codons of the DmmiPEP8 sequence are functional because
they allow the synthesis of GFP (top left picture) whereas the
constructions with mutated ATGs do not produce hardly any GFP (top
right picture).
[0174] Thus, these results suggest that the ORF of the miPEP is
functional.
DETAILED DESCRIPTION OF THE INVENTION
[0175] In the invention, the terms "microRNA", "non-coding
microRNA" and "miRNA" are equivalent and may be used
interchangeably. They define small molecules of RNA of about 21
nucleotides, which are not translated and do not lead to a peptide
or a protein.
[0176] However, in this mature form, the microRNAs perform a
function of regulation of certain genes via post-transcriptional
mechanisms, for example by means of the RISC complex.
[0177] The primary transcript of the microRNA or "pri-miRNA"
corresponds to the RNA molecule obtained directly from
transcription of the DNA molecule. Generally, this primary
transcript undergoes one or more post-transcriptional
modifications, involving for example a particular structure of the
RNA or cleavage of certain portions of the RNA by splicing
phenomena, and which lead to the precursor form of the microRNA or
"pre-miRNA", then to the mature form of the microRNA or
"miRNA".
[0178] The terms "micropeptides" and "miPEPs" (microRNA encoded
PEPtides) are equivalent and may be used interchangeably. They
define a peptide that is encoded by an open reading frame present
on the primary transcript of a microRNA, and which is capable of
modulating the accumulation of said microRNA.
[0179] The micropeptides within the meaning of the present
invention are not to be understood as necessarily being small
peptides, as "micro" does not correspond to the size of the
peptide. According to the invention, a miPEP can also be considered
as a transcription modulator, and in particular a transcription
activator. Such a transcription modulator can operate at the
transcription level to modulate the accumulation of pri-miR,
pre-miR and miR.
[0180] Taking into account the degeneracy of the genetic code, one
and the same micropeptide may be encoded by several nucleotide
sequences. Nucleotide sequences of this kind, differing from one
another by at least one nucleotide but encoding one and the same
peptide, are called "degenerate sequences".
[0181] The terms "open reading frame" or "ORF" are equivalent and
may be used interchangeably. They correspond to a nucleotide
sequence in a DNA or RNA molecule that may potentially encode a
peptide or a protein: said open reading frame begins with a start
codon (the start codon generally encoding a methionine), followed
by a series of codons (each codon encoding an amino acid), and ends
with a stop codon (the stop codon not being translated).
[0182] In the invention, the ORFs may be called specifically
"miORFs" when they are present on the primary transcripts of
microRNA.
[0183] The miORFs as defined in the particular invention may have a
size from 12 to 303 nucleotides and may encode peptides from 3 to
100 amino acids.
[0184] In particular, the miORFs as defined in the invention may
have a size from 15 to 303 nucleotides. As an amino acid is encoded
by a codon of 3 nucleotides, the miORFs from 15 to 303 nucleotides
encode miPEPS from 4 to 100 amino acids.
[0185] In particular, the miORFs have a size of:
[0186] 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 47, 51, 54, 57,
60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105,
108, 111, 114, 117, 120, 123, 126, 129, 132, 135, 138, 141, 15 144,
147, 150, 153, 156, 159, 162, 165, 168, 171, 174, 177, 180, 183,
186, 189, 192, 195, 198, 201, 204, 207, 210, 213, 216, 219, 222,
225, 228, 231, 234, 237, 240, 243, 246, 249, 252, 255, 258, 261,
264, 267, 270, 273, 276, 279, 282, 285, 288, 291, 294, 297, 300 or
303 nucleotides, and encode respectively miPEPs having a size
of:
[0187] 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,
20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 20 30, 31, 32, 33, 34, 35,
36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52,
53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,
70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,
87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 amino
acids.
[0188] In the invention, "accumulation" means the production of a
molecule, such as a microRNA or a micropeptide, in the cell.
[0189] Thus, "modulation" of the accumulation of a molecule in a
cell corresponds to a modification of the quantity of this molecule
present in the cell.
[0190] Moreover, the effect of a miPEP can be observed through the
modulation of the accumulation of the miR, but also through the
modulation of the accumulation of the corresponding pri-miR or
pre-miR.
[0191] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which the
modulation of the accumulation of said microRNA is a decrease or an
increase in the accumulation of said microRNA, in particular an
increase.
[0192] A "decrease in the accumulation" corresponds to a decrease
in the quantity of said molecule in the cell.
[0193] Conversely, an "increase in the accumulation" corresponds to
an increase in the quantity of said molecule in the cell.
[0194] In an advantageous embodiment, the invention relates to a
process for detecting and identifying a miPEP as defined above, in
which the modulation of the accumulation of said microRNA is an
increase in the accumulation of said microRNA.
[0195] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which the
presence of said peptide in the cell results from: [0196] the
introduction of a nucleic acid encoding said peptide into the cell,
or [0197] the introduction of said peptide into the cell.
[0198] In order to characterize a miPEP, it is necessary to have a
cellular model expressing a microRNA in which said peptide to be
tested is present. For this, it is possible to introduce a peptide
into the cell, either by bringing the cell into contact with said
peptide, or by introducing a nucleic acid encoding said peptide
into the cell, and this nucleic acid will then be translated into
peptide within the cell.
[0199] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
open reading frame in step a) is contained in the 5' or 3' portion
of said primary transcript of the microRNA, preferably in the 5'
portion.
[0200] The 5' or 3' portions of the primary transcript of the
microRNA correspond to the terminal portions of the RNA molecule
that are cleaved during maturation of the microRNA.
[0201] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
microRNA is present in a wild-type plant cell.
[0202] In the invention, a wild-type plant cell corresponds to a
plant cell that has not been genetically modified by humans.
[0203] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
microRNA is present in a wild-type animal cell, and in particular a
wild-type human cell or a wild-type Drosophila cell.
[0204] In the invention, a wild-type animal cell corresponds to an
animal cell, and in particular a human cell, that has not been
modified genetically by humans.
[0205] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
specified eukaryotic cell and said eukaryotic cell of the same type
as the aforesaid specified eukaryotic cell, used in step b, are
plant cells of a cruciferous plant such as Arabidopsis thaliana, of
a leguminous plant such as Glycine max (soya), Medicago truncatula
and Medicago sativa (alfalfa) or of a plant of the Solanaceae
family such as Nicotiana benthamiana (tobacco), Solanum tuberosum
(potato), Solanum lycopersicum (tomato) or Solanum melongena
(aubergine).
[0206] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
specified eukaryotic cell and said eukaryotic cell of the same type
as the aforesaid specified eukaryotic cell, used in step b, are
plant cells, preferably cells of Medicago truncatula, Nicotiana
benthamiana or Arabidopsis thaliana.
[0207] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
specified eukaryotic cell and said eukaryotic cell of the same type
as the aforesaid specified eukaryotic cell, used in step b, are
animal cells, preferably human cells or Drosophila cells.
[0208] In the process for detecting and identifying a micropeptide
as defined above, after identifying an ORF that is able to encode a
peptide on the primary transcript of a microRNA, it is necessary to
have a cellular model having said microRNA and said peptide, so as
to be able to demonstrate a possible effect of the peptide on said
microRNA. Two options are therefore conceivable: [0209] the
cellular model in which the miORF has been identified and that in
which the effect of the peptide on the miRNA has been demonstrated
are identical, or [0210] the cellular model in which the miORF has
been identified and that in which the effect of the peptide on the
miRNA has been demonstrated are different.
[0211] In the first option, the cellular model used for observing
an effect of the peptide is the same as that in which the primary
transcript of said microRNA was isolated. In this cellular model,
the specified eukaryotic cells contain said microRNA naturally and
only the peptide to be tested has to be introduced into these
cells. In this context, said microRNA is qualified as "of
endogenous origin" as it exists naturally in the cells.
Nevertheless, other copies of a microRNA of endogenous origin may
be added to a cell, for example by introducing a vector encoding
said microRNA of endogenous origin into the cell.
[0212] In the second option, the cellular model used for observing
an effect of the peptide is different from that in which the
primary transcript of said microRNA was isolated. In this cellular
model, the specified eukaryotic cells contain neither the microRNA,
nor the peptide to be tested. These two elements must therefore be
introduced into these cells. In this context, said microRNA is
qualified as "of exogenous origin" as it does not exist naturally
in the cells.
[0213] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which said
microRNA is of endogenous origin in said eukaryotic cell and in
said eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell, used in step b).
[0214] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above in which said
microRNA is of exogenous origin in said eukaryotic cell and in said
eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell, used in step b), said eukaryotic cells containing
a vector allowing the expression of said microRNA.
[0215] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which the
accumulation of said microRNA is determined using quantitative
RT-PCR or Northern blot.
[0216] In an embodiment, the invention relates to a process for
detecting and identifying a miPEP as defined above, in which the
accumulation of said microRNA is determined using a DNA or RNA
chip.
[0217] The accumulation of said microRNA may be determined using
the techniques of molecular biology for assaying specific nucleic
acid molecules.
[0218] In another aspect, the invention also relates to a process
for detecting and identifying a microRNA in which the sequence of
the primary transcript contains a nucleotide sequence encoding a
miPEP, comprising: [0219] a) a step of detecting an open reading
frame from 15 to 303 nucleotides in length contained in the
sequence of the primary transcript of said microRNA, then [0220] b)
a step of comparison between: [0221] the accumulation of said
microRNA in a specified eukaryotic cell expressing said microRNA,
[0222] in the presence of a peptide encoded by a nucleotide
sequence that is identical or degenerate relative to that of said
open reading frame, said peptide being present in the cell
independently of transcription of the primary transcript of said
microRNA, and [0223] the accumulation of said microRNA in a
eukaryotic cell, of the same type as the aforesaid specified
eukaryotic cell expressing said microRNA, [0224] in the absence of
said peptide,
[0225] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a microRNA the primary transcript of which contains a
nucleotide sequence encoding a micropeptide.
[0226] In particular, the invention relates to a process for
detecting and identifying a microRNA in which the sequence of the
primary transcript contains a nucleotide sequence encoding a
miPEP,
[0227] comprising: [0228] a) a step of detecting an open reading
frame from 15 to 303 nucleotides in length contained in the
sequence of the primary transcript of said microRNA, then [0229] b)
a step of comparison between: [0230] the accumulation of said
microRNA in a specified eukaryotic cell expressing the primary
transcript of said microRNA, [0231] in the presence of a peptide
encoded by a nucleotide sequence that is identical or degenerate
relative to that of said open reading frame, said peptide being
present in the cell independently of transcription of the primary
transcript of said microRNA, and [0232] the accumulation of said
microRNA in a eukaryotic cell, of the same type as the aforesaid
specified eukaryotic cell expressing the primary transcript of said
microRNA, [0233] in the absence of said peptide,
[0234] in which a modulation of the accumulation of said microRNA
in the presence of said peptide relative to the accumulation of
said microRNA in the absence of said peptide indicates the
existence of a microRNA the primary transcript of which contains a
nucleotide sequence encoding a micropeptide.
[0235] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which the
modulation of the accumulation of said microRNA is a decrease or an
increase in the accumulation of said microRNA, in particular an
increase.
[0236] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which the
presence of said peptide in the cell results from: [0237] the
introduction of a nucleic acid encoding said peptide into the cell,
or [0238] the introduction of said peptide into the cell.
[0239] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which
said open reading frame in step a) is contained in the 5' or 3'
portion of said primary transcript of the microRNA, preferably in
the 5' portion. In an embodiment, the invention relates to a
process for detecting and identifying a microRNA as defined above,
in which said microRNA is present in a wild-type plant cell.
[0240] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which
said microRNA is present in a wild-type animal cell, and in
particular a wild-type human cell.
[0241] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which
said eukaryotic cell, and said eukaryotic cell of the same type as
the aforesaid specified eukaryotic cell, used in step b) are plant
cells, preferably cells of Medicago truncatula.
[0242] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which
said eukaryotic cell, and said eukaryotic cell of the same type as
the aforesaid specified eukaryotic cell, used in step b) are animal
cells, preferably Drosophila cells.
[0243] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which
said microRNA is of endogenous origin in said eukaryotic cell and
in said eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell, used in step b).
[0244] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above in which said
microRNA is of exogenous origin in said eukaryotic cell and in said
eukaryotic cell of the same type as the aforesaid specified
eukaryotic cell, used in step b), said eukaryotic cells containing
a vector allowing the expression of said microRNA.
[0245] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which the
accumulation of said microRNA is determined using quantitative
RT-PCR or Northern blot.
[0246] In an embodiment, the invention relates to a process for
detecting and identifying a microRNA as defined above, in which the
accumulation of said microRNA is determined using a DNA or RNA
chip.
[0247] In another aspect, the invention relates to a miPEP as
obtained by implementing the process as defined above.
[0248] More particularly, the invention relates to a miPEP encoded
by a nucleotide sequence as obtained by implementing the process as
defined above. In other words, the invention relates to a miPEP
encoded by a nucleotide sequence detected and identified by
implementing the process as defined above.
[0249] Another aspect of the invention also relates to a miPEP of 3
to 100 amino acids, in particular of 4 to 100 amino acids, in
particular of 4 to 60 amino acids, preferably of 4 to 40 amino
acids, encoded by a nucleotide sequence contained in the primary
transcript of a microRNA, said miPEP being capable of modulating
the accumulation of said microRNA in a eukaryotic cell.
[0250] Another aspect of the invention also relates to a miPEP of 4
to 100 amino acids, or of 3 amino acids, encoded by a nucleotide
sequence contained in the primary transcript of a microRNA, said
miPEP being capable of modulating the accumulation of said microRNA
in a eukaryotic cell.
[0251] Another aspect of the invention also relates to a miPEP of 3
amino acids encoded by a nucleotide sequence contained in the
primary transcript of a microRNA, said miPEP being capable of
modulating the accumulation of said microRNA in a eukaryotic
cell.
[0252] In particular, the miPEP as defined in the invention is
encoded by a miORF of 15 to 303 nucleotides and has a size of 4, 5,
6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,
24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40,
41, 42, 43, 44, 45, 25 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56,
57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73,
74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90,
91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 amino acids, in
particular 5, 8, 10, 18, 19, 23, 37, 50 or 59 amino acids.
[0253] In particular, the miPEP of the invention has a size in the
range from 4 to 10 amino acids, 4 to 20 amino acids, 4 to 30 amino
acids, 4 to 40 amino acids, 4 to 50 amino acids, 4 to 60 amino
acids, 4 to 70 amino acids, 4 to 80 amino acids, 4 to 90 amino
acids, or 4 to 100 amino acids.
[0254] Moreover, it should be noted that several miORFS may be
identified on the primary transcript of a microRNA, indicating that
a primary transcript of microRNA may potentially encode several
miPEPs.
[0255] It should also be noted that the effect of a miPEP is
generally specific to a single microRNA, namely that resulting from
the primary transcript encoding said miPEP.
[0256] The modulation of the microRNA by said miPEP may be
demonstrated after observing a variation in quantities of microRNA
between the cells in the presence and in the absence of the
miPEP.
[0257] In an embodiment, the invention relates to a miPEP as
defined above, said nucleotide sequence being contained in the 5'
or 3' portion of said primary transcript of a microRNA, preferably
in the 5' portion.
[0258] In an embodiment, the invention relates to a miPEP as
defined above, said nucleotide sequence corresponding to the first
open reading frame present on said primary transcript of a
microRNA.
[0259] In an embodiment, the invention relates to a miPEP as
defined above, said miPEP having a basic isoelectric point,
preferably above 8.
[0260] In an embodiment, the invention relates to a miPEP as
defined above, said miPEP having an acidic isoelectric point.
[0261] In an embodiment, the invention relates to a miPEP as
defined above, said miPEP being selected from the group of peptides
consisting of SEQ ID NO: 1 to SEQ ID NO: 104, SEQ ID NO: 375 to SEQ
ID NO: 386, and SEQ ID NO: 355 (Table 1).
[0262] In an embodiment, the invention relates to a miPEP as
defined above, said miPEP being selected from the group of peptides
consisting of: [0263] SEQ ID NO: 1 to SEQ ID NO: 104 and SEQ ID NO:
355, [0264] SEQ ID NO: 375 to SEQ ID NO: 386, or [0265] SEQ ID NO:
424.
[0266] In an embodiment, the invention relates to a miPEP as
defined above, consisting of the amino acid sequence MVT.
[0267] In an embodiment, the invention relates to a miPEP as
defined above, said miPEP being labelled.
[0268] Without restriction, a labeled peptide may be obtained by
fusing said peptide to another peptide sequence, to a fluorescent
marker (GUS, GFP, FAM, FITC, LacZ . . . ), to a signal sequence
(allowing the addressing of the peptide), to a Tag sequence
(allowing purification of miPEP) or to a radioactive isotope
(I.sup.125, P.sup.32, . . . ).
[0269] In an embodiment, the invention relates to a labelled miPEP
as defined above, said labelled miPEP being selected from the group
of peptides consisting of: [0270] SEQ ID NO: 1 to SEQ ID NO: 104
and SEQ ID NO: 355, [0271] SEQ ID NO: 375 to SEQ ID NO: 386, or
[0272] SEQ ID NO: 424.
[0273] In a particular embodiment, the invention relates to the
labelled MtmiPEP171b1 (SEQ ID NO: 59), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0274] In a particular embodiment, the invention relates to the
labelled AtmiPEP164a1 (SEQ ID NO: 24), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0275] In a particular embodiment, the invention relates to the
labelled AtmiPEP165a (SEQ ID NO: 43), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0276] In a particular embodiment, the invention relates to the
labelled AtmiPEP319a1 (SEQ ID NO: 76), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0277] In a particular embodiment, the invention relates to the
labelled AtmiPEP319a2 (SEQ ID NO: 77), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0278] In a particular embodiment, the invention relates to the
labelled AtmiPEP160b1 (SEQ ID NO: 14), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0279] In a particular embodiment, the invention relates to the
labelled MtmiPEP169d (SEQ ID NO: 424), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0280] In a particular embodiment, the invention relates to the
labelled MtmiPEP171e (SEQ ID NO: 63), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0281] In a particular embodiment, the invention relates to the
labelled DmmiPEP1a (SEQ ID NO: 102), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0282] In a particular embodiment, the invention relates to the
labelled DmmiPEP1b (SEQ ID NO: 103), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0283] In a particular embodiment, the invention relates to the
labelled DmmiPEP8 (SEQ ID NO: 104), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0284] In a particular embodiment, the invention relates to the
labelled HsmiPEP155 (SEQ ID NO: 355), preferably labelled by a
fluorescent marker such as GUS, GFP, FAM, FITC or LacZ.
[0285] In another aspect, the invention relates to a nucleic acid
molecule encoding a miPEP as defined above.
[0286] In an embodiment, the invention relates to a nucleic acid
molecule as defined above, said molecule being selected from the
group of nucleic acids consisting of SEQ ID NO: 105 to SEQ ID NO
208, SEQ ID NO: 387 to SEQ ID NO: 399 and SEQ ID NO: 356 (Table
2).
[0287] In an embodiment, the invention relates to a nucleic acid
molecule as defined above, said molecule being selected from the
group of nucleic acids consisting of: [0288] SEQ ID NO: 105 to SEQ
ID NO: 208 and SEQ ID NO: 356, [0289] SEQ ID NO: 387 to SEQ ID NO:
399, or [0290] SEQ ID NO: 425.
[0291] In a particular embodiment, the invention relates to
MtmiPEP171b1 (SEQ ID NO: 59) encoded by the nucleotide sequence
(SEQ ID NO: 163) contained in the primary transcript of miR171b
(SEQ ID NO: 319), said MtmiPEP171b1 being capable of modulating the
accumulation of said miR171b in a eukaryotic cell.
[0292] In a particular embodiment, the invention relates to
AtmiPEP164a1 (SEQ ID NO: 24) encoded by the nucleotide sequence
(SEQ ID NO: 128) contained in the primary transcript of miR164a
(SEQ ID NO: 297), said AtmiPEP164a1 being capable of modulating the
accumulation of said miR164a in a eukaryotic cell.
[0293] In a particular embodiment, the invention relates to
AtmiPEP165a (SEQ ID NO: 43) encoded by the nucleotide sequence (SEQ
ID NO: 147) contained in the primary transcript of miR165a (SEQ ID
NO: 305), said miPEP165a being capable of modulating the
accumulation of said miR165a in a eukaryotic cell.
[0294] In a particular embodiment, the invention relates to
AtmiPEP319a1 (SEQ ID NO: 76) encoded by the nucleotide sequence
(SEQ ID NO: 180) contained in the primary transcript of miR319a
(SEQ ID NO: 331), said AtmiPEP319a1 being capable of modulating the
accumulation of said miR319a in a eukaryotic cell.
[0295] In a particular embodiment, the invention relates to
AtmiPEP319a2 (SEQ ID NO: 77) encoded by the nucleotide sequence
(SEQ ID NO: 181) contained in the primary transcript of miR319a
(SEQ ID NO: 331), said AtmiPEP319a2 being capable of modulating the
accumulation of said miR319a in a eukaryotic cell.
[0296] In a particular embodiment, the invention relates to
AtmiPEP160b1 (SEQ ID NO: 14) encoded by the nucleotide sequence
(SEQ ID NO: 118) contained in the primary transcript of miR160b
(SEQ ID NO: 291), said AtmiPEP160b1 being capable of modulating the
accumulation of said miR160b in a eukaryotic cell.
[0297] In a particular embodiment, the invention relates to
MtmiPEP169d (SEQ ID NO: 424) encoded by the nucleotide sequence
(SEQ ID NO: 425) contained in the primary transcript of miR169d
(SEQ ID NO: 427), said MtmiPEP169d being capable of modulating the
accumulation of said miR169d in a eukaryotic cell.
[0298] In a particular embodiment, the invention relates to
MtmiPEP171e (SEQ ID NO: 63) encoded by the nucleotide sequence (SEQ
ID NO: 167) contained in the primary transcript of miR171e (SEQ ID
NO: 322), said MtmiPEP171e being capable of modulating the
accumulation of said miR171e in a eukaryotic cell.
[0299] In a particular embodiment, the invention relates to
DmmiPEPla (SEQ ID NO: 102) encoded by the nucleotide sequence (SEQ
ID NO: 206) contained in the primary transcript of miR1 (SEQ ID NO:
353), said dmmiPEP1a being capable of modulating the accumulation
of said miR1 in a eukaryotic cell.
[0300] In a particular embodiment, the invention relates to
DmmiPEP1b (SEQ ID NO: 103) encoded by the nucleotide sequence (SEQ
ID NO: 207) contained in the primary transcript of miR1 (SEQ ID NO:
353), said dmmiPEP1b being capable of modulating the accumulation
of said miR1 in a eukaryotic cell.
[0301] In a particular embodiment, the invention relates to
dmmiPEP8 (SEQ ID NO: 104) encoded by the nucleotide sequence (SEQ
ID NO: 208) contained in the primary transcript of miR8 (SEQ ID NO:
354), said dmmiPEP8 being capable of modulating the accumulation of
said miR8 in a eukaryotic cell.
[0302] In a particular embodiment, the invention relates to
HsmiPEP155 (SEQ ID NO: 355) encoded by the nucleotide sequence (SEQ
ID NO: 356) contained in the primary transcript of miR155 (SEQ ID
NO: 358), said HsmiPEP155 being capable of modulating the
accumulation of said miR155 in a eukaryotic cell.
[0303] In another aspect, the invention relates to an isolated
peptide, or an isolated and purified peptide, or a synthetic
peptide or a recombinant peptide, comprising or consisting of a
sequence identical to that of a miPEP, said miPEP in particular
being present naturally in a plant, or in an animal, such as
humans.
[0304] In another aspect, the invention relates to a vector
comprising at least one nucleic acid molecule as defined above.
[0305] In another aspect, the invention also relates to the use of
at least: [0306] a miPEP as defined above, [0307] a nucleic acid
encoding said miPEP, or [0308] a vector containing said nucleic
acid,
[0309] for modulating the expression of at least one gene in a
specified eukaryotic cell,
[0310] said specified eukaryotic cell being capable of expressing a
microRNA, the primary transcript of which contains at least one
nucleotide sequence encoding said at least one miPEP and the
accumulation of which is modulated by said at least one miPEP,
[0311] the expression of said at least one gene being regulated by
said microRNA.
[0312] In another aspect, the invention also relates to the use of
at least: [0313] a miPEP of 4 to 100 amino acids, preferably of 4
to 40 amino acids, encoded by a nucleotide sequence contained in
the primary transcript of a microRNA, said miPEP being capable of
modulating the accumulation of said microRNA in a eukaryotic cell,
[0314] a nucleic acid encoding said miPEP, or [0315] a vector
containing said nucleic acid,
[0316] for modulating the expression of at least one gene in a
specified eukaryotic cell, said specified eukaryotic cell being
capable of expressing a microRNA, the primary transcript of which
contains at least one nucleotide sequence encoding said at least
one miPEP and the accumulation of which is modulated by said at
least one miPEP,
[0317] the expression of said at least one gene being regulated by
said microRNA.
[0318] In another aspect, the invention also relates to the use of
at least: [0319] a miPEP of 4 to 100 amino acids, or of 3 amino
acids, encoded by a nucleotide sequence contained in the primary
transcript of a microRNA, said miPEP being capable of modulating
the accumulation of said microRNA in a eukaryotic cell, [0320] a
nucleic acid encoding said miPEP, or [0321] a vector containing
said nucleic acid,
[0322] for modulating the expression of at least one gene in a
specified eukaryotic cell, said specified eukaryotic cell being
capable of expressing a microRNA, the primary transcript of which
contains at least one nucleotide sequence encoding said at least
one miPEP and the accumulation of which is modulated by said at
least one miPEP,
[0323] the expression of said at least one gene being regulated by
said microRNA.
[0324] In another aspect, the invention also relates to the use of
at least: [0325] a miPEP of 3 amino acids, encoded by a nucleotide
sequence contained in the primary transcript of a microRNA, said
miPEP being capable of modulating the accumulation of said microRNA
in a eukaryotic cell, [0326] a nucleic acid encoding said miPEP, or
[0327] a vector containing said nucleic acid,
[0328] for modulating the expression of at least one gene in a
specified eukaryotic cell,
[0329] said specified eukaryotic cell being capable of expressing a
microRNA, the primary transcript of which contains at least one
nucleotide sequence encoding said at least one miPEP and the
accumulation of which is modulated by said at least one miPEP,
[0330] the expression of said at least one gene being regulated by
said microRNA.
[0331] The invention is based on the surprising observation made by
the inventors that it is possible to modulate the expression of one
or more target genes of one and the same microRNA by modulating the
accumulation of said microRNA using a miPEP.
[0332] In an embodiment, the invention relates to the use as
defined above in which said specified eukaryotic cell is a plant
cell.
[0333] In an embodiment, the invention relates to the use as
defined above in which said specified eukaryotic cell is a plant
cell of a crucifer such as Arabidopsis thaliana, of a leguminous
plant such as Glycine max (soya), Medicago truncatula and Medicago
sativa (alfalfa) or of a plant of the Solanaceae family such as
Nicotiana benthamiana (tobacco), Solanum tuberosum (potato),
Solanum lycopersicum (tomato) or Solanum melongena (aubergine).
[0334] In an embodiment, the invention relates to the use as
defined above in which said specified eukaryotic cell is an animal
cell, in particular human.
[0335] In an embodiment, the invention relates to the use as
defined above in which said specified eukaryotic cell is an animal
cell, in particular human, said miPEP not being used for surgical
or therapeutic treatment of the human body or animal body, nor for
modifying the genetic identity of a human being.
[0336] In an embodiment, the invention relates to the use as
defined above in which said specified eukaryotic cell is an animal
cell, said miPEP being used for surgical or therapeutic treatment
of the human body or animal body.
[0337] In an embodiment, the invention relates to the use as
defined above in which said microRNA and said gene are of
endogenous origin in said specified eukaryotic cell.
[0338] In an embodiment, the invention relates to the use as
defined above in which said microRNA and said gene are of exogenous
origin in said specified eukaryotic cell, said specified eukaryotic
cell containing at least one vector allowing the expression of said
microRNA and of said gene.
[0339] In the invention, the expressions "of endogenous origin" and
"of exogenous origin" are used for distinguishing said microRNAs
and/or the genes of different species, in view of the conservation
of the sequences between species.
[0340] Thus, the term "of endogenous origin" indicates that the
microRNA and/or gene may be present naturally in the cell in
question. Other copies of the microRNA and/or of the gene of
endogenous origin may nevertheless be added artificially to the
cell in question, for example by cloning.
[0341] Conversely, the term "of exogenous origin" indicates that
the microRNA and/or the gene are never present naturally in the
cell in question. It is a microRNA and/or a gene identified in
another cellular type or in an organism of another species; this
microRNA and/or this gene are therefore necessarily introduced
artificially into the cell in question.
[0342] In the invention, a genetically transformed cell may
therefore contain 2 groups of microRNAs and/or of genes potentially
similar in terms of sequence, one of endogenous origin and the
other of exogenous origin.
[0343] In an embodiment, the invention relates to the use as
defined above in which the primary transcript of the miRNA and said
gene are of exogenous origin in said specified eukaryotic cell,
said specified eukaryotic cell containing at least one vector
allowing the expression of the primary transcript of the
microRNA.
[0344] In an embodiment, the invention relates to the use as
defined above in which the primary transcript of the miRNA is
encoded by a vector introduced into the cell artificially.
[0345] In an embodiment, the invention relates to the use as
defined above in which said miPEP is selected from the group of
peptides consisting of SEQ ID NO: 1 to SEQ ID NO: 104, SEQ ID NO:
375 to SEQ ID NO: 386 and SEQ ID NO: 355 (Table 1).
[0346] In an embodiment, the invention relates to the use as
defined above in which said miPEP is selected from the group of
peptides consisting of: [0347] SEQ ID NO: 1 to SEQ ID NO: 104 and
SEQ ID NO: 355, [0348] SEQ ID NO: 375 to SEQ ID NO: 386, or [0349]
SEQ ID NO: 424.
[0350] In an embodiment, the invention relates to the use as
defined above in which said miPEP is selected from MtmiPEP171b1
(SEQ ID NO: 59), AtmiPEP164a1 (SEQ ID NO: 24), AtmiPEP165a (SEQ ID
NO: 43), AtmiPEP319a1 (SEQ ID NO: 76) and AtmiPEP319a2 (SEQ ID NO:
77).
[0351] In an embodiment, the invention relates to the use as
defined above in which said miPEP is selected from MtmiPEP171b1
(SEQ ID NO: 59), AtmiPEP164a1 (SEQ ID NO: 24), AtmiPEP165a (SEQ ID
NO: 43), AtmiPEP319a1 (SEQ ID NO: 76), AtmiPEP319a2 (SEQ ID NO:
77), AtmiPEP160b1 (SEQ ID NO: 14), MtmiPEP171e (SEQ ID NO: 63) and
MtmiPEP169d (SEQ ID NO: 424),In an embodiment, the invention
relates to the use as defined above in which said miPEP is selected
from DmmiPEPla (SEQ ID NO: 102),
[0352] DmmiPEPlb (SEQ ID NO: 103) and DmmiPEP8 (SEQ ID NO:
104).
[0353] In an embodiment, the invention relates to the use as
defined above in which said miPEP is HsmiPEP155a (SEQ ID NO:
355).
[0354] In an embodiment, the invention relates to the use as
defined above in which said nucleic acid is selected from the group
of nucleic acids consisting of SEQ ID NO: 105 to SEQ ID NO: 208 and
SEQ ID NO: 356 (Table 2).
[0355] In an embodiment, the invention relates to the use as
defined above in which said nucleic acid is selected from the group
of nucleic acids consisting of: [0356] SEQ ID NO: 105 to SEQ ID NO:
208 and SEQ ID NO: 356, [0357] SEQ ID NO: 387 to SEQ ID NO: 399, or
[0358] SEQ ID NO: 425.
[0359] In an embodiment, the invention relates to the use as
defined above in which said nucleic acid is selected from miORF171b
(SEQ ID NO: 163), miORF164a1 (SEQ ID NO: 128), miORF165a (SEQ ID
NO: 147), miORF319a1 (SEQ ID NO: 180) and miORF319a2 (SEQ ID NO:
181).
[0360] In an embodiment, the invention relates to the use as
defined above in which said nucleic acid is selected from miORF171b
(SEQ ID NO: 163), miORF164a1 (SEQ ID NO: 128), miORF165a (SEQ ID
NO: 147), miORF319a1 (SEQ ID NO: 180), miORF319a2 (SEQ ID NO: 181),
miORF160b1 (SEQ ID NO: 118), miORF169d (SEQ ID NO: 425) and
miORF171e (SEQ ID NO: 167).
[0361] In an embodiment, the invention relates to the use as
defined above in which said nucleic acid is selected from miORF1a
(SEQ ID NO: 206), miORFlb (SEQ ID NO: 207) and miORF8 (SEQ ID NO:
208).
[0362] In an embodiment, the invention relates to the use as
defined above in which said nucleic acid is selected from miORF155
(SEQ ID NO: 356).
[0363] In an embodiment, the invention relates to the use as
defined above in which said microRNA is selected from the group of
nucleic acids consisting of SEQ ID NO: 282 to SEQ ID NO: 354 and
SEQ ID NO: 358.
[0364] In an embodiment, the invention relates to the use as
defined above in which said microRNA is selected from the group of
nucleic acids consisting of: [0365] SEQ ID NO: 282 to SEQ ID NO:
354 and SEQ ID NO: 358, [0366] SEQ ID NO: 412 to SEQ ID NO: 423, or
[0367] SEQ ID NO: 427.
[0368] In an embodiment, the invention relates to the use as
defined above in which said microRNA is selected from miR171b (SEQ
ID NO: 319), miR165a (SEQ ID NO: 305) and miR319a (SEQ ID NO:
331).
[0369] In an embodiment, the invention relates to the use as
defined above in which said microRNA is selected from miR171b (SEQ
ID NO: 319), miR164a (SEQ ID NO: 297), miR165a (SEQ ID NO: 305),
miR319a (SEQ ID NO: 331), miR160b (SEQ ID NO: 291), miR171e (SEQ ID
NO: 322) and miR169d (SEQ ID NO: 427).
[0370] In an embodiment, the invention relates to the use as
defined above in which said microRNA is selected from miRla (SEQ ID
NO: 353) and miR8 (SEQ ID NO: 354).
[0371] In an embodiment, the invention relates to the use as
defined above in which said microRNA is selected from miR155 (SEQ
ID NO: 358).
[0372] In another aspect, the invention relates in particular to a
process for modulating the expression of a gene regulated by a
microRNA in a eukaryotic cell,
[0373] comprising carrying out a step of accumulation of a miPEP in
said eukaryotic cell, [0374] said miPEP having: [0375] a size from
3 to 100 amino acids, preferably 4 to 20 amino acids, and [0376] a
peptide sequence identical to that encoded by a nucleotide sequence
contained in the primary transcript of a microRNA regulating the
expression of said gene, and [0377] being capable of modulating the
accumulation of said microRNA,
[0378] in which the accumulation of said miPEP in said eukaryotic
cell induces a modulation of the expression of said gene relative
to the expression of said gene without accumulation of said
miPEP.
[0379] In another aspect, the invention relates in particular to a
process for modulating the expression of a gene regulated by a
microRNA in a eukaryotic cell,
[0380] comprising carrying out a step of accumulation of a miPEP in
said eukaryotic cell, [0381] said miPEP having: [0382] a size from
4 to 100 amino acids, or of 3 amino acids, and [0383] a peptide
sequence identical to that encoded by a nucleotide sequence
contained in the primary transcript of a microRNA regulating the
expression of said gene, and [0384] being capable of modulating the
accumulation of said microRNA,
[0385] in which the accumulation of said miPEP in said eukaryotic
cell induces a modulation of the expression of said gene relative
to the expression of said gene without accumulation of said
miPEP.
[0386] In another aspect, the invention relates in particular to a
process for modulating the expression of a gene regulated by a
microRNA in a eukaryotic cell,
[0387] comprising carrying out a step of accumulation of a miPEP in
said eukaryotic cell, [0388] said miPEP having: [0389] a size of 3
amino acids, and [0390] a peptide sequence identical to that
encoded by a nucleotide sequence contained in the primary
transcript of a microRNA regulating the expression of said gene,
and [0391] being capable of modulating the accumulation of said
microRNA,
[0392] in which the accumulation of said miPEP in said eukaryotic
cell induces a modulation of the expression of said gene relative
to the expression of said gene without accumulation of said
miPEP.
[0393] In particular, the invention relates to a process for
modulating the expression of a gene regulated by a microRNA in a
eukaryotic cell,
[0394] comprising carrying out a step of accumulation of a miPEP in
said eukaryotic cell, [0395] said miPEP having: [0396] a size from
4 to 100 amino acids, preferably 4 to 20 amino acids, and [0397] a
peptide sequence identical to that encoded by a nucleotide sequence
contained in the primary transcript of a microRNA regulating the
expression of said gene, and [0398] being capable of modulating the
accumulation of said microRNA,
[0399] in which the accumulation of said miPEP in said eukaryotic
cell induces a modulation of the expression of said gene relative
to the expression of said gene without accumulation of said
miPEP.
[0400] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above, in which the
accumulation of said miPEP in the cell results from: [0401]
introduction of a nucleic acid encoding said miPEP into the cell,
or [0402] introduction of said miPEP into the cell.
[0403] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
eukaryotic cell is a plant cell.
[0404] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
eukaryotic cell is an animal cell and in particular a human
cell.
[0405] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
eukaryotic cell is an animal cell and in particular a human cell,
said process not being used for surgical or therapeutic treatment
of the human body or animal body, nor for modifying the genetic
identity of a human being.
[0406] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
microRNA and said gene are of endogenous origin in said eukaryotic
cell.
[0407] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
microRNA and said gene are of exogenous origin in said eukaryotic
cell, said eukaryotic cell containing at least one vector allowing
the expression of said microRNA and of said gene.
[0408] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
miPEP is selected from the group of peptides consisting of SEQ ID
NO: 1 to SEQ ID NO: 104, SEQ ID NO: 375 to SEQ ID NO: 386 and SEQ
ID NO: 355.
[0409] In an embodiment, the invention relates to a process for
modulating the expression of a gene as defined above in which said
miPEP is selected from the group of peptides consisting of: [0410]
SEQ ID NO: 1 to SEQ ID NO: 104 and SEQ ID NO: 355, [0411] SEQ ID
NO: 375 to SEQ ID NO: 386, or [0412] SEQ ID NO: 424.
[0413] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR171b (SEQ ID NO: 319) in a eukaryotic cell, comprising carrying
out a step of accumulation of MtmiPEP171b1 (SEQ ID NO : 59) in said
eukaryotic cell,
[0414] in which the accumulation of said MtmiPEP171b1 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said MtmiPEP171b1.
[0415] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR171b (SEQ ID NO: 319) in a eukaryotic cell, comprising carrying
out a step of accumulation of MtmiPEP171b1 (SEQ ID NO: 59) in said
eukaryotic cell,
[0416] in which the accumulation of said MtmiPEP171b1 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said MtmiPEP171b1,
[0417] in which said gene is selected from the genes HAM1
(accession No. MtGI9-TC114268) and HAM2 (accession No.
MtGI9-TC120850) (accession numbers according to the database
Medicago truncatula Gene Expression Atlas "MtGEA").
[0418] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR164a (SEQ ID NO: 297) in a eukaryotic cell,
[0419] comprising carrying out a step of accumulation of
AtmiPEP165a1 (SEQ ID NO: 24) in said eukaryotic cell,
[0420] in which the accumulation of said AtmiPEP164a1 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said AtmiPEP164a1.
[0421] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR165a (SEQ ID NO: 305) in a eukaryotic cell,
[0422] comprising carrying out a step of accumulation of
AtmiPEP165a (SEQ ID NO: 43) in said eukaryotic cell,
[0423] in which the accumulation of said AtmiPEP165a in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said AtmiPEP165a.
[0424] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR319a (SEQ ID NO: 331) in a eukaryotic cell, comprising carrying
out a step of accumulation of AtmiPEP319a1 (SEQ ID NO: 76) in said
eukaryotic cell,
[0425] in which the accumulation of said AtmiPEP319a1 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said AtmiPEP319a1.
[0426] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR319a (SEQ ID NO: 331) in a eukaryotic cell,
[0427] comprising carrying out a step of accumulation of
AtmiPEP319a2 (SEQ ID NO: 77) in said eukaryotic cell,
[0428] in which the accumulation of said AtmiPEP319a2 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said AtmiPEP319a2.
[0429] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR160b (SEQ ID NO: 291) in a eukaryotic cell,
[0430] comprising carrying out a step of accumulation of
AtmiPEP160b1 (SEQ ID NO: 14) in said eukaryotic cell,
[0431] in which the accumulation of said AtmiPEP160b1 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said AtmiPEP160b1.
[0432] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR169d (SEQ ID NO: 427) in a eukaryotic cell,
[0433] comprising carrying out a step of accumulation of
MtmiPEP169d (SEQ ID NO: 424) in said eukaryotic cell,
[0434] in which the accumulation of said MtmiPEP169d in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said MtmiPEP169d.
[0435] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by
miR171e (SEQ ID NO: 322) in a eukaryotic cell,
[0436] comprising carrying out a step of accumulation of
MtmiPEP171e (SEQ ID NO: 63) in said eukaryotic cell,
[0437] in which the accumulation of said MtmiPEP171e in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said MtmiPEP171e.
[0438] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by miR1
(SEQ ID NO: 353) in a eukaryotic cell,
[0439] comprising carrying out a step of accumulation of DmmiPEP1a
(SEQ ID NO: 102) in said eukaryotic cell,
[0440] in which the accumulation of said DmmiPEPla in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said DmmiPEP1a.
[0441] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by miRl
(SEQ ID NO: 353) in a eukaryotic cell,
[0442] comprising carrying out a step of accumulation of DmmiPEP1b
(SEQ ID NO: 103) in said eukaryotic cell,
[0443] in which the accumulation of said DmmiPEP1b in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said DmmiPEP1b.
[0444] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by miR8
(SEQ ID NO: 354) in a eukaryotic cell,
[0445] comprising carrying out a step of accumulation of DmmiPEP8
(SEQ ID NO: 104) in said eukaryotic cell,
[0446] in which the accumulation of said DmmiPEP8 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said DmmiPEP8.
[0447] In a particular embodiment, the invention relates to a
process for modulating the expression of a gene regulated by miR155
(SEQ ID NO: 358) in a eukaryotic cell,
[0448] comprising carrying out a step of accumulation of hsmiPEP155
(SEQ ID NO: 355) in said eukaryotic cell,
[0449] in which the accumulation of said hsmiPEP155 in said
eukaryotic cell induces a modulation of the expression of said gene
relative to the expression of said gene without accumulation of
said hsmiPEP155.
[0450] In another aspect, the invention relates to a modified
eukaryotic cell containing a peptide identical to a miPEP as
defined above, said peptide being present in said eukaryotic cell
independently of transcription of the primary transcript of the
microRNA bearing the nucleotide sequence encoding said miPEP.
[0451] In the invention, by the term "modified eukaryotic cell" is
meant that said eukaryotic cell contains a miPEP introduced into
the cell artificially, whether as a peptide, or via a vector
encoding said miPEP.
[0452] In addition to a miPEP introduced artificially in the cell,
a modified eukaryotic cell according to the invention also contains
at least one nucleic acid corresponding to the miORF encoding said
miPEP.
[0453] In addition to a miPEP introduced artificially in the cell
and of the miORF, a modified eukaryotic cell according to the
invention also contains at least one miR, the primary transcript of
which contains said miORF.
[0454] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, in which said microRNA is of
endogenous origin.
[0455] In another embodiment, the invention relates to a modified
eukaryotic cell as defined above in which said microRNA is of
exogenous origin, said modified eukaryotic cell containing a vector
allowing the expression of said microRNA.
[0456] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, said cell being a plant cell.
[0457] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, in which said plant cell is a
cell of Medicago truncatula or of Arabidopsis thaliana, and said
peptide is selected from the group of peptides consisting of:
[0458] SEQ ID NO: 1 to SEQ ID NO: 101, [0459] SEQ ID NO: 375 to SEQ
ID NO: 386, or [0460] SEQ ID NO: 424.
[0461] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, in which said plant cell is a
cell of Medicago truncatula or of Arabidopsis thaliana, and said
peptide is selected from the group consisting of: SEQ ID NO: 43,
SEQ ID NO: 59 and SEQ ID NO: 77.
[0462] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, in which said plant cell is a
cell of Medicago truncatula or of Arabidopsis thaliana, and said
peptide is selected from the group consisting of: SEQ ID NO: 59,
SEQ ID NO: 24, SEQ ID NO: 43, SEQ ID NO: 76, SEQ ID NO: 77, SEQ ID
NO: 14, SEQ ID NO: 424 and SEQ ID NO: 63.
[0463] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, said cell being an animal
cell.
[0464] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, in which said animal cell is a
Drosophila cell and said peptide is selected from the group of
peptides consisting of SEQ ID NO: 102, SEQ ID NO: 103 and SEQ ID
NO: 104.
[0465] In an embodiment, the invention relates to a modified
eukaryotic cell as defined above, in which said animal cell is a
human cell and said peptide consists of SEQ ID NO: 355.
[0466] In another aspect, the invention relates to a plant
comprising at least one modified eukaryotic cell as defined
above.
[0467] In another aspect, the invention also relates to a non-human
animal organism comprising at least one modified eukaryotic cell as
defined above.
[0468] In another aspect, the invention relates to a composition
comprising at least: [0469] a miPEP as defined above, [0470] a
nucleic acid encoding said miPEP, or [0471] a vector containing
said nucleic acid.
[0472] In another aspect, the invention relates to a composition
comprising at least one miPEP selected from the group consisting
of: [0473] SEQ ID NO: 1 to SEQ ID NO: 101, [0474] SEQ ID NO: 375 to
SEQ ID NO: 386, or [0475] SEQ ID NO: 424.
[0476] In another aspect, the invention relates to a pesticide
composition comprising at least: [0477] a miPEP as defined above,
[0478] a nucleic acid encoding said miPEP, or [0479] a vector
containing said nucleic acid.
[0480] In another aspect, the invention relates to a
phytopharmaceutical composition comprising at leaste: [0481] a
miPEP as defined above, [0482] a nucleic acid encoding said miPEP,
or [0483] a vector containing said nucleic acid.
[0484] In another aspect, the invention relates to an elicitor
composition comprising at least: [0485] a miPEP as defined above,
[0486] a nucleic acid encoding said miPEP, or [0487] a vector
containing said nucleic acid.
[0488] "Elicitor composition" denotes a composition capable of
endowing the plant with better capacity for symbiosis or better
resistance to different stresses whether they are of the nature of
thermal stress, water stress or chemical stress.
[0489] For this purpose, the invention also relates to compositions
acting on the growth (inhibition of growth or conversely growth
promotion) and the physiology (better capacity for mycorrhization,
nodule formation, better tolerance of different stresses) of the
plant.
[0490] In particular, the invention relates to compositions for
promoting plant growth.
[0491] In another aspect, the invention relates to a herbicide
composition comprising at least: [0492] a miPEP as defined above,
[0493] a nucleic acid encoding said miPEP, or [0494] a vector
containing said nucleic acid.
[0495] In another aspect, the invention relates to an insecticide
composition comprising at least: [0496] a miPEP as defined above,
[0497] a nucleic acid encoding said miPEP, or [0498] a vector
containing said nucleic acid.
[0499] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP164a as
active ingredient, said miPEP164a preferably consisting of SEQ ID
NO: 24.
[0500] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP319a as
active ingredient, said miPEP319a preferably consisting of SEQ ID
NO: 76.
[0501] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP171b as
active ingredient, said miPEP171b preferably consisting of SEQ ID
NO: 59.
[0502] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP165a as
active ingredient, said miPEP165a preferably consisting of SEQ ID
NO: 43.
[0503] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP319a2 as
active ingredient, said miPEP319a2 preferably consisting of SEQ ID
NO: 77.
[0504] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP160b1 as
active ingredient, said miPEP160b1 preferably consisting of SEQ ID
NO: 14.
[0505] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP169d as
active ingredient, said miPEP169d preferably consisting of SEQ ID
NO: 424.
[0506] In another aspect, the invention relates to a composition,
in particular a phytosanitary composition, comprising miPEP171e as
active ingredient, said miPEP171e preferably consisting of SEQ ID
NO: 63.The solubility properties of the miPEPs are in particular
determined by their amino acid composition. The hydrophilic miPEPs
can be dissolved and packaged in aqueous solutions, such as water.
The hydrophobic miPEPs can be dissolved and packaged in solvents,
such as organic solvents.
[0507] For treatment of plants with the miPEPS, the organic
solvents are solvents that are non-toxic to the plants in small
quantities, i.e. they do not have any harmful effect on the
development of the plant. Non-limitatively, the organic solvents
may be selected from acetonitrile and acetic acid.
[0508] The miPEPs can also be dissolved and packaged in mixtures of
organic solvents, such as for example a mixture of acetonitrile and
acetic acid. In particular, the miPEPs may be dissolved in a
solution comprising 50% acetonitrile, 10% acetic acid and 40% water
(volume/volume/volume).
[0509] Preferably, miPEPs 164a and 165a are dissolved in water, and
miPEPs 171b and 319a are dissolved in a solution comprising 50%
acetonitrile, 10% acetic acid and 40% water
(volume/volume/volume).
[0510] Non-limitatively, the compositions, the pesticide
compositions, the phytopharmaceutical compositions, the herbicide
compositions and the insecticide compositions as defined above may
comprise 10.sup.-9 M to 10.sup.-4 M of miPEP, in particular
10.sup.-9 M, 10.sup.-8 M, 10.sup.-7 M, 10.sup.-6 M, 10.sup.-5M or
10.sup.-4 M of miPEP.
[0511] Compositions of higher or lower concentration may also be
provided depending on the applications envisaged. For example,
compositions comprising 10.sup.-1 M to 10.sup.-3 M of miPEP, in
particular 10.sup.-1 M, 10.sup.-2 M or 10.sup.-3 M of miPEP, may be
envisaged in the case where the miPEP has to be administered to the
plant by spreading.
[0512] In another aspect, the invention relates to the use of a
composition as defined above, as a herbicide for eradicating plants
or slowing their growth, preferably as a herbicide specific to a
species or to a genus of plants.
[0513] In another aspect, the invention relates to the use of a
composition as defined above, as a phytopharmaceutical agent,
[0514] for promoting the growth and/or development of plants,
[0515] in particular for modulating the physiological parameters of
a plant, in particular the biomass, foliar surface area, flowering,
fruit size, production and/or selection of plant seeds, in
particular for controlling the parthenocarpy or the monoecism of a
plant, or for modifying the physiological parameters of plant
seeds, in particular germination, establishment of the root system
and resistance to water stress, [0516] or for preventing or
treating plant diseases,
[0517] in particular for promoting resistance to infectious
diseases.
[0518] In another aspect, the invention relates to the use of a
composition as defined above, for modulating the physiological
parameters of a plant, in particular biomass, foliar surface area,
or fruit size.
[0519] In an embodiment, the invention relates to the use of a
composition as defined above, for thinning of orchards in order to
increase fruit size.
[0520] In an embodiment, the invention relates to the use of a
composition as defined above, for production and/or selection of
plant seeds, said composition being used for controlling the
parthenocarpy or the monoecism of a plant.
[0521] In an embodiment, the invention relates to the use of a
composition as defined above, said composition being administered
to said plant via the leaves or via the roots.
[0522] In an embodiment, the invention relates to the use of a
composition as defined above, for production and/or selection of
plant seeds.
[0523] In an embodiment, the invention relates to the use of a
composition as defined above, in which said composition is used for
modifying the physiological parameters of said plant seeds, in
particular establishment of the root system, germination and
resistance to water stress.
[0524] In an embodiment, the invention relates to the use of a
composition as defined above, in which said composition is applied
by coating or film-coating of said plant seeds.
[0525] In another aspect, the invention relates to the use of a
composition as defined above, as a pesticide, for eradicating
organisms that are harmful to plants or that might be classified as
such,
[0526] in particular as insecticide, arachnicide, molluscicide or
rodenticide.
[0527] In an embodiment, the invention relates to the use of a
composition as defined above, as insecticide.
[0528] In an embodiment, the invention relates to the use of a
composition as defined above, for eradicating insect pests.
[0529] In an embodiment, the invention relates to the use of a
composition as defined above, for eradicating animal species
classified as harmful or liable to be classified as such, in
particular the Muridae, in particular the rat.
[0530] In an embodiment, the invention relates to the use of a
composition as defined above, as pesticide for eradicating
organisms harmful to plants or liable to be classified as such,
[0531] in particular as insecticide, arachnicide, molluscicide, or
rodenticide,
[0532] in particular by application of said composition to a plant
or to a support in contact with the plant.
[0533] In another aspect, the invention relates to the use of a
composition as defined above, in which said composition is applied
to a plant to protect it against insect pests.
[0534] In another aspect, the invention relates to the use of a
peptide for promoting the growth of a plant, said peptide being
introduced into the plant, said peptide having an amino acid
sequence comprising or consisting of a sequence identical to that
of a miPEP naturally present in said plant,
[0535] said miPEP naturally present in said plant being a peptide
of 4 to 100 amino acids the sequence of which is encoded by an open
reading frame located on the primary transcript of a miRNA, said
miPEP being capable of modulating the accumulation of said miRNA in
said plant, said miRNA regulating the expression of at least one
gene involved in the development of the vegetative or reproductive
parts of the plant, in particular the roots, stem, leaves or
flowers.
[0536] The inventors have surprisingly found that the use of
peptides the sequence of which comprises or consists of a sequence
identical to that of miPEPs encoded on the primary transcripts of
miRNAs makes it possible to promote the growth of the plants.
[0537] In the invention, the term "plant" refers generally to the
whole or part of a plant irrespective of its stage of development
(including the plant in the form of a seed or a young shoot), to
one or more organs of the plant (for example the leaves, roots,
stem, flowers), to one or more cells of the plant, or to a cluster
of cells of the plant.
[0538] In the invention, the term "growth" refers to the
development of the whole or part of a plant over time. The growth
of the plant may thus be determined and quantified by monitoring
developmental parameters observable for certain parts, cells or
organs of the plant, such as the leaves, roots, stems or
flowers.
[0539] Non-limitatively, the parameters for determining and
quantifying the growth of a plant may in particular be: [0540] the
size, surface area, volume, mass and the number of leaves, [0541]
the size and number of flowers, [0542] the size of the stem (or
spike), [0543] the length and number of roots, [0544] the earliness
of germination, [0545] the earliness of budding, [0546] the
earliness of floral induction (or floral transition), [0547] or
also the number of cells.
[0548] In the case of leguminous plants, plant growth may also be
linked to the rate of nodulation, or also to the size and number of
nodules on the roots.
[0549] Moreover, in the invention, the expression "promote plant
growth", or "improve plant growth", indicates: [0550] either an
acceleration of development (such as for example a larger leaf size
for a plant at a given point in time relative to a reference
plant), [0551] or an increase in development (such as for example a
larger leaf size for a plant that cannot be attained by a reference
plant), [0552] or an acceleration and an increase in the
development of the plant.
[0553] It is important to note that the use according to the
invention has the advantage of being ecological, in comparison with
the chemical methods used conventionally in horticulture or in
agriculture, as the miPEP is a peptide that is present naturally in
the plant.
[0554] The invention also relates to the use of a miPEP introduced
into a plant for promoting its growth,
[0555] said miPEP introduced being a peptide comprising, or
consisting of, a sequence identical to that of a miPEP naturally
present in said plant,
[0556] said miPEP naturally present is a peptide of 4 to 100 amino
acids, the sequence of which is encoded by an open reading frame
located at 5' on the primary transcript of a miRNA,
[0557] said miPEP being capable of modulating the accumulation of
said miRNA in said plant,
[0558] said miRNA regulating the expression of at least one gene
involved in the development of the vegetative or reproductive parts
of the plant, in particular the roots, stem, leaves or flowers,
[0559] the sum total of the quantity of said miPEP introduced and
that of said miPEP naturally present being strictly greater than
the quantity of said miPEP naturally present.
[0560] In the invention, the expression "miPEP introduced" refers
to a miPEP introduced into the plant artificially as opposed to the
"miPEP present naturally in the plant". The introduction of a miPEP
into the plant therefore involves a technological step, which is
not a natural phenomenon and corresponds neither to crossing, nor
to selection.
[0561] The miPEP introduced may be either a peptide produced
outside of the plant (for example an isolated and/or purified
peptide, a synthetic peptide or a recombinant peptide), or a
peptide produced in the plant following the non-natural
introduction of a nucleic acid encoding said miPEP into said
plant.
[0562] The plant into which the miPEP has not been introduced has a
basal quantity of said miPEP, which corresponds to the quantity of
said miPEP naturally present. The use of a miPEP comprising, or
consisting of, a sequence identical to that of said miPEP leads to
an increase in the total quantity of miPEP, which modulates the
accumulation of the miRNA the primary transcript of which contains
the sequence encoding said miPEP.
[0563] Moreover, the miPEP introduced is present in the plant and
its introduction has no impact on its stability.
[0564] In an embodiment, the invention relates to the use as
defined above, in which said gene, involved in the development of
the vegetative or reproductive parts of the plant, is selected from
the group consisting of: NAC1 (Accession No. AT1G56010.1), NAC4
(Accession No. AT5G07680.1), NAC5 (Accession No. AT5G61430.1), CUC1
(Accession No. AT3G15170.1), CUC2 (Accession No. AT5G53950.1), TCP3
(Accession No. AT1G53230.1) and TCP4 (Accession No. AT3G15030.1)
(accession numbers according to the database The Arabidopsis
Information Resource "TAIR").
[0565] In particular, the invention relates to the use as defined
above, in which said gene involved in the development of the
vegetative or reproductive parts of the plant is selected from the
group consisting of: NAC1, NAC4, NAC5, CUC1 and CUC2.
[0566] In an embodiment, the invention relates to the use as
defined above, in which said gene involved in the development of
the vegetative or reproductive parts of the plant is selected from
the group consisting of: TCP3 and TCP4.
[0567] In an embodiment, the invention relates to the use as
defined above, in which said miRNA is selected from miR164a and
mir319a.
[0568] In an embodiment, the invention relates to the use as
defined above, in which said miR is selected from miR164a, miR319a,
miR171b, miR165a, miR160b, miR169d and miR171e.
[0569] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is selected from AtmiPEP164al,
AtmiPEP319al, AtmiPEP319a2, MtmiPEP171b1, AtmiPEP165a1,
AtmiPEP160b1, MtmiPEP169d, MtmiPEP 171e.
[0570] In particular, the invention relates to the use as defined
above, in which said miR164a has a nucleotide sequence consisting
of SEQ ID NO: 297.
[0571] In particular, the invention relates to the use as defined
above, in which said miR164a has a nucleotide sequence having at
least 80% identity, preferably at least 90% identity, with the
nucleotide sequence SEQ ID NO: 297.
[0572] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is AtmiPEP164al, in particular
in which said AtmiPEP164a1 has an amino acid sequence consisting of
SEQ ID NO: 24.
[0573] In particular, the invention relates to the use as defined
above, in which said miR319a has a nucleotide sequence consisting
of SEQ ID NO: 331.
[0574] In particular, the invention relates to the use as defined
above, in which said miR319a has a nucleotide sequence having at
least 80% identity, preferably at least 90% identity, with the
nucleotide sequence SEQ ID NO: 331.
[0575] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is AtmiPEP319a1, in particular
in which said AtmiPEP319a1 has an amino acid sequence consisting of
SEQ ID NO: 76.
[0576] In an embodiment, the invention relates to the use as
defined above, in which said plant is a crucifer such as
Arabidopsis thaliana, a leguminous plant such as Glycine max
(soya), Medicago truncatula and Medicago sativa (alfalfa) or a
plant of the Solanaceae family such as Nicotiana benthamiana
(tobacco), Solanum tuberosum (potato), Solanum lycopersicum
(tomato) or Solanum melongena (aubergine).
[0577] In an embodiment, the invention relates to the use as
defined above, in which said plant is a crucifer.
[0578] In an embodiment, the invention relates to the use as
defined above, in which said plant is Arabidopsis thaliana.
[0579] In an embodiment, the invention relates to the use as
defined above, for promoting the growth of an Arabidopsis thaliana
plant, in which AtmiPEP164a1 is introduced into said Arabidopsis
thaliana plant, said AtmiPEP164a1 also being naturally present in
said Arabidopsis thaliana plant,
[0580] said AtmiPEP164a1 introduced being a peptide the sequence of
which comprises or consists of a sequence identical to that of said
AtmiPEP164a1 naturally present, said sequence of AtmiPEP164a1
naturally present being encoded by an open reading frame located at
5' on the primary transcript of the miR164a, which miR164a controls
the expression of at least one gene involved in the development of
the vegetative or reproductive parts of Arabidopsis thaliana,
[0581] the sum total of the quantity of said AtmiPEP164a1
introduced and that of said AtmiPEP164a1 naturally present being
strictly greater than the quantity of said AtmiPEP164a1 naturally
present in said Arabidopsis thaliana plant.
[0582] In an embodiment, the invention relates to the use as
defined above, for promoting the growth of an Arabidopsis thaliana
plant, in which the AtmiPEP319a1 is introduced into said
Arabidopsis thaliana plant, said AtmiPEP319a1 also being naturally
present in said Arabidopsis thaliana plant,
[0583] said AtmiPEP319a1 introduced being a peptide the sequence of
which comprises or consists of a sequence identical to that of said
AtmiPEP319a1 naturally present, said sequence of the AtmiPEP319a1
naturally present being encoded by an open reading frame located at
5' on the primary transcript of the miR319a, which miR319a controls
the expression of at least one gene involved in the development of
the vegetative or reproductive parts of Arabidopsis thaliana,
[0584] the sum total of the quantity of said AtmiPEP319a1
introduced and that of said AtmiPEP319a1 naturally present being
strictly greater than the quantity of said AtmiPEP319a1 naturally
present in said Arabidopsis thaliana plant.
[0585] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is introduced into the plant
externally, preferably by watering, by spraying or by adding a
fertilizer, a compost, a culture substrate or an inert support.
[0586] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is introduced by watering and by
spraying.
[0587] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is introduced by watering and by
adding a fertilizer.
[0588] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is introduced by spraying and by
adding a fertilizer.
[0589] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is introduced, by watering, by
spraying and by adding a fertilizer.
[0590] The inventors have in fact unexpectedly found that it is
possible to apply a composition comprising a miPEP directly to the
plant in order to modulate the accumulation of the corresponding
miRNA in the plant, which indicates that the miPEP is captured by
the plant.
[0591] In an embodiment, the invention relates to the use as
defined above, in which the plant is treated with a composition
comprising 10.sup.-9 M to 10.sup.-4 M of said miPEP, in particular
10.sup.-9 M, 10.sup.-8 M, 10.sup.-7 M, 10.sup.-6 M, 10.sup.-5 M or
10.sup.-4 M of said miPEP.
[0592] Preferably, the compositions have a concentration from
10.sup.-8 M to 10.sup.-5 M for application by watering or by
spraying on the plant.
[0593] In addition, compositions of higher or lower concentration
may be envisaged for treating the plant with the miPEP. As a
non-limitative example, compositions of higher concentration
comprising 10.sup.-1 M to 10.sup.-3 M, in particular 10.sup.-2 M of
miPEP, may be used in the case where the miPEP introduced
exogenously is administered to the plant by spreading.
[0594] In an embodiment, the invention relates to the use as
defined above, in which said miPEP is introduced into the plant by
means of a nucleic acid encoding said miPEP, said nucleic acid
being introduced into the plant. P In an embodiment, the invention
relates to the use as defined above, in which the size of the stem
is increased in the plant into which said miPEP has been introduced
relative to the size of the stem of an identical plant of the same
age into which no miPEP has been introduced, or relative to the
size of the stem of an identical plant of the same age into which
said miPEP has not been introduced.
[0595] In an embodiment, the invention relates to the use as
defined above, in which the number of leaves is increased in the
plant into which said miPEP has been introduced relative to the
number of leaves of an identical plant of the same age into which
no miPEP has been introduced, or relative to the number of leaves
of an identical plant of the same age into which said miPEP has not
been introduced.
[0596] In an embodiment, the invention relates to the use as
defined above, in which the size of the leaves is increased in the
plant into which said miPEP has been introduced relative to the
size of the leaves of an identical plant of the same age into which
no miPEP has been introduced, or relative to the size of the leaves
of an identical plant of the same age into which said miPEP has not
been introduced.
[0597] In an embodiment, the invention relates to the use as
defined above, in which the number of roots is increased in the
plant into which said miPEP has been introduced relative to the
number of roots of an identical plant of the same age into which no
miPEP has been introduced, or relative to the number of roots of an
identical plant of the same age into which said miPEP has not been
introduced.
[0598] In an embodiment, the invention relates to the use as
defined above, in which the length of the roots is increased in the
plant into which said miPEP has been introduced relative to the
length of the roots of an identical plant of the same age into
which no miPEP has been introduced, or relative to the length of
the roots of an identical plant of the same age into which said
miPEP has not been introduced.
[0599] In an embodiment, the invention relates to the use as
defined above, in which the rate of nodulation is increased in the
plant into which said miPEP has been introduced relative to the
rate of nodulation of an identical plant of the same age into which
no miPEP has been introduced, or relative to the rate of nodulation
of an identical plant of the same age into which said miPEP has not
been introduced.
[0600] In an embodiment, the invention relates to the use as
defined above, in which the number of nodules is increased in the
plant into which said miPEP has been introduced relative to the
number of nodules of an identical plant of the same age into which
no miPEP has been introduced, or relative to the number of nodules
of an identical plant of the same age into which said miPEP has not
been introduced.
[0601] The increase in the parameters for determining and
quantifying growth in the plant into which the miPEP has been
introduced (such as the size of the stem, the number and size of
the leaves, the number and length of the roots, the rate of
nodulation or also the number of nodules on the roots) is
preferably demonstrated by comparison with an identical plant (i.e.
a plant of the same species and/or variety), of the same age and
grown under the same conditions but into which no miPEP has been
introduced.
[0602] In another aspect, the invention relates to a process for
promoting the growth of a plant, comprising a step of introducing a
miPEP into a plant, said miPEP also being present naturally in said
plant,
[0603] said miPEP introduced being a peptide of 4 to 100 amino
acids the sequence of which comprises or consists of a sequence
identical to that of said miPEP naturally present, said sequence of
the miPEP naturally present being encoded by an open reading frame
located at 5' on the primary transcript of a miRNA, said miPEP
being capable of modulating the accumulation of said miRNA, said
miRNA regulating the expression of at least one gene involved in
the development of the vegetative or reproductive parts of the
plant, in particular the roots, stem, leaves or flowers,
[0604] the sum total of the quantity of said miPEP introduced and
that of said miPEP naturally present being strictly greater than
the quantity of said miPEP naturally present.
[0605] In an embodiment, the invention relates to a process as
defined above, in which said gene involved in the development of
the vegetative or reproductive parts of the plant is selected from
the group consisting of: NAC1 (Accession No. AT1G56010.1), NAC4
(Accession No. AT5G07680.1), NAC5 (Accession No. AT5G61430.1), CUC1
(Accession No. AT3G15170.1), CUC2 (Accession No. AT5G53950.1), TCP3
(Accession No. AT1G53230.1) and TCP4 (Accession No.
AT3G15030.1).
[0606] In particular, the invention relates to a process as defined
above, in which said gene involved in the development of the
vegetative or reproductive parts of the plant is selected from the
group consisting of: NAC1, NAC4, NAC5, CUC1 and CUC2.
[0607] In an embodiment, the invention relates to a process as
defined above, in which said gene involved in the development of
the vegetative or reproductive parts of the plant is selected from
the group consisting of: TCP3 and TCP4.
[0608] In an embodiment, the invention relates to a process as
defined above, in which said miR is selected from miR164a, miR319a,
miR171b, miR165a, miR160b, miR169d and miR171e.
[0609] In an embodiment, the invention relates to a process as
defined above, in which said miPEP is selected from AtmiPEP164a1,
AtmiPEP319a1, AtmiPEP319a2, MtmiPEP171b1, AtmiPEP165a1,
AtmiPEP160b1, MtmiPEP169d, MtmiPEP171e.
[0610] In an embodiment, the invention relates to a process as
defined above, in which said miRNA is miR164a, in particular in
which said miR164a has a nucleotide sequence consisting of SEQ ID
NO: 297.
[0611] In an embodiment, the invention relates to a process as
defined above, in which said miPEP is AtmiPEP164a1, in particular
in which said AtmiPEP164a1 has an amino acid sequence consisting of
SEQ ID NO: 24.
[0612] In an embodiment, the invention relates to a process as
defined above, in which said miRNA is miR319a, in particular in
which said miR319a has a nucleotide sequence consisting of SEQ ID
NO: 331.
[0613] In an embodiment, the invention relates to a process as
defined above, in which said miPEP is AtmiPEP319a1, in particular
in which said AtmiPEP319a1 has an amino acid sequence consisting of
SEQ ID NO: 76.
[0614] In an embodiment, the invention relates to a process as
defined above, in which said plant is a crucifer such as
Arabidopsis thaliana, a leguminous plant such as Glycine max
(soya), Medicago truncatula and Medicago sativa (alfalfa) or a
plant of the Solanaceae family such as Nicotiana benthamiana
(tobacco), Solanum tuberosum (potato), Solanum lycopersicum
(tomato) or Solanum melongena (aubergine).
[0615] In an embodiment, the invention relates to a process as
defined above, in which said plant is a crucifer.
[0616] In an embodiment, the invention relates to a process as
defined above, in which said plant is Arabidopsis thaliana.
[0617] In an embodiment, the invention relates to a process as
defined above, for promoting the growth of an Arabidopsis thaliana
plant, in which AtmiPEP164a1 is introduced into said Arabidopsis
thaliana plant, said AtmiPEP164a1 also being naturally present in
said Arabidopsis thaliana plant,
[0618] said AtmiPEP164a1 introduced being a peptide comprising or
consisting of a sequence identical to that of said AtmiPEP164a1
naturally present, where the AtmiPEP164a1 naturally present is a
peptide of 4 to 100 amino acids the sequence of which is encoded by
an open reading frame located at 5' on the primary transcript of
the miR164a,
[0619] said AtmiPEP164a1 being capable of increasing the
accumulation of said miR164a, where said miR164a regulates the
expression of at least one gene involved in the development of the
vegetative or reproductive parts of Arabidopsis thaliana,
[0620] the sum total of the quantity of said AtmiPEP164a1
introduced and that of said AtmiPEP164a1 naturally present being
strictly greater than the quantity of said AtmiPEP164a1 naturally
present.
[0621] In an embodiment, the invention relates to a process as
defined above, for promoting the growth of an Arabidopsis thaliana
plant, in which AtmiPEP319a1 is introduced into said Arabidopsis
thaliana plant, said AtmiPEP319a1 also being naturally present in
said Arabidopsis thaliana plant,
[0622] said AtmiPEP319a1 introduced being a peptide comprising or
consisting of a sequence identical to that of said AtmiPEP319a1
naturally present, where the AtmiPEP319a1 naturally present is a
peptide of 4 to 100 amino acids the sequence of which is encoded by
an open reading frame located at 5' on the primary transcript of
the miR319a,
[0623] said AtmiPEP319a1 being capable of increasing the
accumulation of said miR319a, where miR319a regulates the
expression of at least one gene involved in the development of the
vegetative or reproductive parts of Arabidopsis thaliana,
[0624] the sum total of the quantity of said AtmiPEP319a1
introduced and that of said AtmiPEP319a1 naturally present being
strictly greater than the quantity of said AtmiPEP319a1 naturally
present.
[0625] In an embodiment, the invention relates to a process as
defined above, in which said miPEP is introduced into the plant
externally, preferably by watering, by spraying or by adding a
fertilizer, a compost, a culture substrate or an inert support.
[0626] In an embodiment, the invention relates to a process as
defined above, in which said miPEP is administered to the plant in
the form of a composition comprising 10.sup.-9 M to 10.sup.-4 M of
said miPEP, in particular 10.sup.-9, 10.sup.-8, 10.sup.-7,
10.sup.-6, 10.sup.-5 or 10.sup.-4 M of said miPEP.
[0627] In an embodiment, the invention relates to a process as
defined above, in which said miPEP is introduced into the plant by
means of a nucleic acid encoding said miPEP, said nucleic acid
being introduced into the plant.
[0628] In an embodiment, the invention relates to a process as
defined above, in which the size of the stem is increased in the
plant into which said miPEP has been introduced relative to the
size of the stem of an identical plant of the same age into which
no miPEP has been introduced, or relative to the size of the stem
of an identical plant of the same age into which said miPEP has not
been introduced.
[0629] In an embodiment, the invention relates to a process as
defined above, in which the number of leaves is increased in the
plant into which said miPEP has been introduced relative to the
number of leaves of an identical plant of the same age into which
no miPEP has been introduced, or relative to the number of leaves
of an identical plant of the same age into which said miPEP has not
been introduced.
[0630] In an embodiment, the invention relates to a process as
defined above, in which the size of the leaves is increased in the
plant into which said miPEP has been introduced relative to the
size of the leaves of an identical plant of the same age into which
no miPEP has been introduced, or relative to the size of the leaves
of an identical plant of the same age into which said miPEP has not
been introduced.
[0631] In an embodiment, the invention relates to a process as
defined above, in which the number of roots is increased in the
plant into which said miPEP has been introduced relative to the
number of roots of an identical plant of the same age into which no
miPEP has been introduced, or relative to the number of roots of an
identical plant of the same age into which said miPEP has not been
introduced.
[0632] In an embodiment, the invention relates to a process as
defined above, in which the length of the roots is increased in the
plant into which said miPEP has been introduced relative to the
length of the roots of an identical plant of the same age into
which no miPEP has been introduced, or relative to the length of
the roots of an identical plant of the same age into which said
miPEP has not been introduced.
[0633] In an embodiment, the invention relates to a process as
defined above, in which the rate of nodulation is increased in the
plant into which said miPEP has been introduced relative to the
rate of nodulation of an identical plant of the same age into which
no miPEP has been introduced, or relative to the rate of nodulation
of an identical plant of the same age into which said miPEP has not
been introduced.
[0634] In an embodiment, the invention relates to a process as
defined above, in which the number of nodules is increased in the
plant into which said miPEP has been introduced relative to the
number of nodules of an identical plant of the same age into which
no miPEP has been introduced, or relative to the number of nodules
of an identical plant of the same age into which said miPEP has not
been introduced.
[0635] In another aspect, the invention relates to a plant into
which a miPEP has been introduced according to the use or the
process for promoting the growth of a plant described above. In
another aspect, the invention relates to a process for producing a
transgenic plant comprising: a) a step of introducing a nucleic
acid encoding a miPEP of 4 to 100 amino acids into a plant, or into
at least one cell of said plant, under conditions allowing the
expression of said miPEP,
[0636] said miPEP also being naturally present in said plant, said
miPEP naturally present being a peptide the sequence of which is
encoded by an open reading frame located at 5' on the primary
transcript of a miR, said miPEP being capable of modulating the
accumulation of said miR in the plant, said miR regulating the
expression of at least one gene involved in the development of the
vegetative or reproductive parts of the plant, in particular the
roots, stem, leaves or flowers, and [0637] b) a step of culturing
the plant, or at least one cell of said plant, obtained in step a)
under conditions allowing a transgenic plant to be obtained.
[0638] In another aspect, the invention relates to a process for
producing a transgenic plant comprising: [0639] a) a step of
introducing a nucleic acid encoding a miPEP of 4 to 100 amino
acids, or of 3 amino acids, into a plant, or into at least one cell
of said plant, under conditions allowing the expression of said
miPEP,
[0640] said miPEP also being naturally present in said plant, said
miPEP naturally present being a peptide the sequence of which is
encoded by an open reading frame located at 5' on the primary
transcript of a miR, said miPEP being capable of modulating the
accumulation of said miR in the plant, said miR regulating the
expression of at least one gene involved in the development of the
vegetative or reproductive parts of the plant, in particular the
roots, stem, leaves or flowers, and [0641] b) a step of culturing
the plant, or at least one cell of said plant, obtained in step a)
under conditions allowing a transgenic plant to be obtained.
[0642] In another aspect, the invention relates to a process for
producing a transgenic plant comprising: [0643] a) a step of
introducing a nucleic acid encoding a miPEP of 3 amino acids, into
a plant, or into at least one cell of said plant, under conditions
allowing the expression of said miPEP,
[0644] said miPEP also being naturally present in said plant, said
miPEP naturally present being a peptide the sequence of which is
encoded by an open reading frame located at 5' on the primary
transcript of a miR, said miPEP being capable of modulating the
accumulation of said miR in the plant, said miR regulating the
expression of at least one gene involved in the development of the
vegetative or reproductive parts of the plant, in particular the
roots, stem, leaves or flowers, and
[0645] b) a step of culturing the plant, or at least one cell of
said plant, obtained in step a) under conditions allowing a
transgenic plant to be obtained.
[0646] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said
transgenic plant obtained in step b) has improved growth relative
to an identical plant in which said nucleic acid has not been
introduced.
[0647] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the
expression of saif miPEP encoded by the nucleic acid introduced in
the plant results in an improved growth relative to an identical
plant in which said nucleic acid has not been introduced.
[0648] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which step a) is
carried out using a vector containing said nucleic acid, preferably
a plasmid.
[0649] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the
expression of said nucleic acid of step a) is placed under the
control of a strong promoter, preferably a constitutive strong
promoter such as the 35S promoter.
[0650] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said gene
involved in the development of the vegetative or reproductive parts
of the plant is selected from the group consisting of: NAC1
(Accession No. AT1G56010.1), NAC4 (Accession No. AT5G07680.1), NAC5
(Accession No. AT5G61430.1), CUC1 (Accession No. AT3G15170.1), CUC2
(Accession No. AT5G53950.1), TCP3 (Accession No. AT1G53230.1) and
TCP4 (Accession No. AT3G15030.1).
[0651] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said miPEP
has an amino acid sequence comprising or consisting of a sequence
selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:
101 and SEQ ID NO: 375 to SEQ ID NO: 386.
[0652] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said miPEP
has an amino acid sequence comprising or consisting of a sequence
selected from the group consisting of: [0653] SEQ ID NO: 1 to SEQ
ID NO: 101, [0654] SEQ ID NO: 375 to SEQ ID NO: 386, or [0655] SEQ
ID NO: 424.
[0656] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said miRNA
is miR164a, in particular in which said miR164a has a nucleotide
sequence consisting of SEQ ID NO: 297.
[0657] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said miPEP
is AtmiPEP164a1, in particular in which said AtmiPEP164a1 has an
amino acid sequence consisting of SEQ ID NO: 24.
[0658] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said miRNA
is miR319a, in particular in which said miR319a has a nucleotide
sequence consisting of SEQ ID NO: 331.
[0659] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said miPEP
is AtmiPEP319a1, in particular in which said AtmiPEP319a1 has an
amino acid sequence consisting of SEQ ID NO: 76.
[0660] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said
nucleic acid introduced in step a) comprises a nucleotide sequence
selected from SEQ ID NO: 128 and SEQ ID NO: 180.
[0661] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said plant
is a crucifer such as Arabidopsis thaliana, a leguminous plant such
as Glycine max (soya), Medicago truncatula and Medicago sativa
(alfalfa) or a plant of the Solanaceae family such as Nicotiana
benthamiana (tobacco), Solanum tuberosum (potato), Solanum
lycopersicum (tomato) or Solanum melongena (aubergine).
[0662] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said
transgenic plant is a crucifer.
[0663] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which said
transgenic plant is Arabidopsis thaliana.
[0664] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, comprising: [0665]
a) a step of introducing a nucleic acid containing the nucleotide
sequence SEQ ID NO: 128, encoding AtmiPEP164a1 consisting of the
amino acid sequence SEQ ID NO: 24, into an Arabidopsis thaliana
plant, or into at least one cell of said Arabidopsis thaliana
plant, under conditions allowing the expression of
AtmiPEP164a1,
[0666] said AtmiPEP164a1 also being naturally present in said
Arabidopsis thaliana plant, said miPEP naturally present being a
peptide the sequence of which is encoded by an open reading frame
located at 5' on the primary transcript of miR164a, said
AtmiPEP164a1 being capable of modulating the accumulation of said
miR164, where miR164a controls the expression of at least one gene
involved in the development of the vegetative or reproductive parts
of Arabidopsis thaliana, and [0667] b) a step of culturing the
plant, or at least one cell of said plant, obtained in step a)
under conditions allowing a transgenic Arabidopsis thaliana plant
to be obtained.
[0668] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, comprising: [0669]
a) a step of introducing a nucleic acid containing the nucleotide
sequence SEQ ID NO: 180, encoding AtmiPEP319a1 consisting of the
amino acid sequence SEQ ID NO: 76, into an Arabidopsis thaliana
plant, or into at least one cell of said Arabidopsis thaliana
plant, under conditions allowing the expression of
AtmiPEP319a1,
[0670] said AtmiPEP319a1 also being naturally present in said
Arabidopsis thaliana plant, said miPEP naturally present being a
peptide the sequence of which is encoded by an open reading frame
located at 5' on the primary transcript of the miR319a, said
AtmiPEP319a1 being capable of modulating the accumulation of said
miR319, which miR319a regulates the expression of at least one gene
involved in the development of the vegetative or reproductive parts
of Arabidopsis thaliana, and [0671] b) a step of culturing the
plant, or at least one cell of said plant, obtained in step a)
under conditions allowing a transgenic Arabidopsis thaliana plant
to be obtained.
[0672] In an embodiment, the invention relates to a process of
production as defined above, in which said miPEP is introduced into
the plant by means of a nucleic acid encoding said miPEP, said
nucleic acid being introduced into the plant.
[0673] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the size of
the stem is increased in the plant into which said miPEP has been
introduced relative to the size of the stem of an identical plant
of the same age into which no miPEP has been introduced, or
relative to the size of the stem of an identical plant of the same
age into which said miPEP has not been introduced.
[0674] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the number
of leaves is increased in the plant into which said miPEP has been
introduced relative to the number of leaves of an identical plant
of the same age into which no miPEP has been introduced, or
relative to the number of leaves of an identical plant of the same
age into which said miPEP has not been introduced.
[0675] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the size of
the leaves is increased in the plant into which said miPEP has been
introduced relative to the size of the leaves of an identical plant
of the same age into which no miPEP has been introduced, or
relative to the size of the leaves of an identical plant of the
same age into which said miPEP has not been introduced.
[0676] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the number
of roots is increased in the plant into which said miPEP has been
introduced relative to the number of roots of an identical plant of
the same age into which no miPEP has been introduced, or relative
to the number of roots of an identical plant of the same age into
which said miPEP has not been introduced.
[0677] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the length
of the roots is increased in the plant into which said miPEP has
been introduced relative to the length of the roots of an identical
plant of the same age into which no miPEP has been introduced, or
relative to the length of the roots of an identical plant of the
same age into which said miPEP has not been introduced.
[0678] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the rate of
nodulation is increased in the plant into which said miPEP has been
introduced relative to the rate of nodulation of an identical plant
of the same age into which no miPEP has been introduced, or
relative to the rate of nodulation of an identical plant of the
same age into which said miPEP has not been introduced.
[0679] In an embodiment, the invention relates to a process for
producing a transgenic plant as defined above, in which the number
of nodules is increased in the plant into which said miPEP has been
introduced relative to the number of nodules of an identical plant
of the same age into which no miPEP has been introduced, or
relative to the number of nodules of an identical plant of the same
age into which said miPEP has not been introduced.
[0680] In an aspect, the invention also relates to a transgenic
plant as obtained by the process of production as defined
above.
[0681] In another aspect, the invention relates to a transgenic
plant containing a vector encoding a miPEP, said miPEP being also
naturally present in said plant,
[0682] said miPEP naturally present being a peptide of which the
sequence is encoded by an open reading frame located in 5' portion
on the primary transcript of a miR, said miPEP being capable of
modulating the accumulation of said miR in the plant, said miR
regulating the expression of at least one gene,
[0683] the expression of said miPEP encoded by the vector
introduced in the plant resulting in a modulation of the expression
of said gene relative to an identical plant in which said nucleic
acid has not been introduced.
[0684] In an embodiment, the invention relates to a transgenic
plant as defined above, in which said gene is involved in the
development of the vegetative or reproductive parts of the plant,
in particular the roots, stem, leaves or flowers, and the
expression of said miPEP encoded by the nucleic acid introduced in
the plant results in an improved growth of the transgenic plant
relative to an identical plant in which said nucleic acid has not
been introduced.
[0685] In an embodiment, the invention relates to a transgenic
plant as defined above, in which said miPEP is selected from the
group consisting of: [0686] SEQ ID NO: 1 to SEQ ID NO: 101, [0687]
SEQ ID NO: 375 to SEQ ID NO: 386, or [0688] SEQ ID NO: 424.
[0689] In another aspect, the invention relates to a transgenic
plant comprising a vector,
[0690] said vector containing a nucleic acid enabling the
expression of a primary transcript of a miR,
[0691] said primary transcript of a miR containing an open reading
frame encoding a miPEP,
[0692] said miPEP being capable of modulating the accumulation of
said miR in said plant, said miR regulating the expression of at
least one gene in said plant,
[0693] the expression of the miPEP encoded by the vector introduced
in the plant resulting in a modulation of the expression of said
gene relative to an identical plant in which said nucleic acid has
not been introduced.
[0694] In an embodiment, the invention relates to a transgenic
plant as defined above in which said nucleic acid is of endogenous
origin.
[0695] If said nucleic acid is of endogenous origin, at least one
copy of said nucleic acid is already present naturally in the
plant, independently of the vector introduced in the plant.
[0696] In an embodiment, the invention relates to a transgenic
plant as defined above in which said nucleic acid is of exogenous
origin.
[0697] If said nucleic acid is of exogenous origin, no copy of said
nucleic acid is already present naturally in the plant
[0698] In an embodiment, the invention relates to a transgenic
plant as defined above in which said gene is involved in the
development of the vegetative or reproductive parts of the plant,
in particular the roots, stem, leaves or flowers, and the
expression of said miPEP encoded by the nucleic acid introduced in
the plant results in an improved growth of the transgenic plant
relative to an identical plant in which said nucleic acid has not
been introduced.
[0699] In an embodiment, the invention relates to a transgenic
plant as defined above in which said miPEP is selected from the
group consisting of: [0700] SEQ ID NO: 1 to SEQ ID NO: 101, [0701]
SEQ ID NO: 375 to SEQ ID NO: 386, or [0702] SEQ ID NO: 424.
[0703] In another aspect, the invention relates to a composition
comprising, in combination, a quantity of seeds of a plant and a
quantity of a peptide the sequence of which comprises or consists
of a sequence identical to that of a miPEP naturally present in
said plant.
[0704] In an embodiment, the invention relates to a composition as
defined above in which said miPEP is selected from the group
consisting of: [0705] SEQ ID NO: 1 to SEQ ID NO: 101, [0706] SEQ ID
NO: 375 to SEQ ID NO: 386, or [0707] SEQ ID NO: 424.
[0708] In an embodiment, the invention relates to a composition
comprising, in combination, a quantity of seeds of a plant, in
particular A. thaliana, and a quantity of a peptide the sequence of
which comprises or consists of a sequence identical to that of
AtmiPEP164a1.
[0709] In an embodiment, the invention relates to a composition
comprising, in combination, a quantity of seeds of a plant, in
particular A. thaliana, and a quantity of a peptide the sequence of
which comprises or consists of a sequence identical to that of
AtmiPEP319a1.
[0710] In an embodiment, the invention relates to a composition
comprising, in combination, a quantity of seeds of a plant, in
particular M truncatula, and a quantity of a peptide the sequence
of which comprises or consists of a sequence identical to that of
MtmiPEP171b.
[0711] In an embodiment, the invention relates to a composition as
defined above, formulated so as to form a coated seed.
[0712] Coating may be carried out by the processes used
conventionally in the agri-food industry and may be obtained using
a material able to disaggregate in a solvent or in the ground, such
as a binder or clay.
[0713] According to the invention, coating may be used for example
for conferring particular properties on a composition of miPEP, or
on a composition of seeds in combination with a miPEP.
[0714] In an embodiment, the invention relates to a composition as
defined above, formulated so as to form a coated seed comprising
MtmiPEP171b.
[0715] In an embodiment, the invention relates to a composition as
defined above, formulated so as to form a coated seed comprising
AtmiPEP164a1.
[0716] In an embodiment, the invention relates to a composition as
defined, above formulated so as to form a coated seed comprising
AtmiPEP319a1.
[0717] In another aspect, the invention relates to a pharmaceutical
composition comprising at least: [0718] a miPEP as defined above
[0719] a nucleic acid encoding said miPEP, or [0720] a vector
containing said nucleic acid.
[0721] and a pharmaceutically acceptable excipient.In another
aspect, the invention relates to a composition comprising at least:
[0722] a miPEP as defined above [0723] a nucleic acid encoding said
miPEP, or [0724] a vector containing said nucleic acid.
[0725] for use as a medicament, in particular for humans or for
animals.
[0726] The use of the compositions of the invention is applicable
in human medicine and in veterinary medicine.
[0727] In another aspect, the invention relates to a composition
comprising at least: [0728] a miPEP as defined above [0729] a
nucleic acid encoding said miPEP, or [0730] a vector containing
said nucleic acid,
[0731] for use in the prevention and/or treatment of a disease
involving deregulation of the expression of a gene of the
patient,
[0732] the expression of said gene being regulated by a microRNA
the accumulation of which is modulated by said miPEP.
[0733] In an embodiment, the invention relates to the composition
as defined above in which said disease is selected from cancer,
diabetes, obesity, infectious diseases and neurodegenerative
diseases.
[0734] In an embodiment, the invention relates to the composition
as defined above in which said disease is selected from: cancer,
diabetes, obesity, infectious diseases, neurodegenerative diseases,
cardiovascular diseases and autoimmune diseases.
[0735] In an embodiment, the invention relates to the composition
as defined above in which said disease is selected from: cancer,
diabetes, obesity, infectious diseases, neurodegenerative diseases,
or from cardiovascular diseases and autoimmune diseases.
[0736] In an embodiment, the invention relates to the composition
as defined above in which said disease is selected from
cardiovascular diseases and autoimmune diseases.
[0737] In another aspect, the invention relates to a composition
comprising at least: [0738] a miPEP as defined above, [0739] a
nucleic acid encoding said miPEP, or [0740] a vector containing
said nucleic acid,
[0741] for use in the prevention and/or treatment of an infection
of an animal or of a human with a parasitic organism,
[0742] said parasitic organism having a gene the expression of
which is regulated by a microRNA the accumulation of which is
modulated by said miPEP.
[0743] In another aspect, the invention relates to a miPEP from 4
to 100 amino acids, or of 3 amino acids, encoded by a nucleotidic
sequence contained in the preimary transcript of a microRNA, said
miPEP being capable of modulating the accumulation of said microRNA
in an eukaryotic cell, for its use a medicament.
[0744] In another aspect, the invention relates to an antibody
specifically recognizing a miPEP.
[0745] In particular, the invention relates to an antibody
specifically recognizing AtmiPEP165a.
[0746] In particular, the invention relates to an antibody
specifically recognizing MtmiPEP171b.
[0747] In particular, the invention relates to an antibody
specifically recognizing AtmiPEP164a1.
[0748] In particular, the invention relates to an antibody
specifically recognizing AtmiPEP319a1.
[0749] Such an antibody may be obtained by a process known to a
person skilled in the art, such as for example by injecting said
miPEP into a non-human animal in order to trigger an immunization
reaction and the production of antibodies by said animal.
[0750] In another aspect, the invention relates to a process of
immunolocalization of a miPEP comprising a step of labelling a
biological sample from a plant with an antibody specifically
recognizing a miPEP.
[0751] In particular, the invention relates to a process of
immunolocalization of AtmiPEP165a using an antibody specifically
recognizing AtmiPEP165a.
[0752] In particular, the invention relates to a process of
immunolocalization of MtmiPEP171b using an antibody specifically
recognizing MtmiPEP171b.
[0753] In particular, the invention relates to a process of
immunolocalization of AtmiPEP164a1 using an antibody specifically
recognizing AtmiPEP164a1.
[0754] In particular, the invention relates to a process of
immunolocalization of MtmiPEP319a1 using an antibody specifically
recognizing AtmiPEP319a1.
[0755] In another aspect, the invention relates to an antibody
specifically recognizing a miPEP according to the invention, for
its use as a medicament.
[0756] In another aspect, the invention relates a pharmaceutical
composition comprising an antibody specifically recognizing a miPEP
according to the invention and a pharmaceutically acceptable
excipient.
[0757] In another aspect, the invention relates to a protocol for
producing a recombinant peptide, the sequence of which comprises or
consists of a sequence identical to that of a miPEP as defined
above, comprising a step of transforming an organism with an
expression vector encoding said recombinant peptide.
[0758] In an embodiment, said organism is selected from the group
comprising bacteria, yeasts, fungi (other than yeasts), animal
cells, plants and animals.
[0759] In an embodiment, said organism is Escherichia coli.
[0760] In particular, the invention relates to a protocol for
producing a recombinant peptide as defined above, comprising the
following steps: [0761] binding the nucleic acid encoding said
recombinant peptide to a nucleic acid encoding a tag, such as GST,
[0762] introducing the expression vector containing said nucleic
acid encoding said recombinant peptide into the bacterium E. coli,
[0763] culturing the bacterium E. coli containing the expression
vector in LB medium preferably up to an OD between 0.2 and 0.4,
[0764] inducing production of the recombinant peptide with IPTG,
preferably for 4 to 5 hours, [0765] centrifuging and lysing the E.
coli bacteria, [0766] filtering the supernatant, [0767] purifying
said recombinant peptide on a glutathione sepharose affinity
column, [0768] if necessary, cleaving the GST with a protease.
[0769] All the sequences of the miPEPs, miORFs, miRNAs and primary
transcripts of miRNAs are presented in Tables 1, 2, 3, 4, 5 and
6.
[0770] Table 7 presents an analysis of the polymorphism of the DNA
sequence of the different regions of pri-miR171b (a haplotype is
defined when it differs by at least one amino acid from the other
haplotypes).
TABLE-US-00001 TABLE 1 List of potential miPEPs (miPEPs) miPEP
Target genes of Sequence of (pI/size MW) miRNA Organism the miRNA
the miPEP SEQ ID AtmiPEP156a1 miR156a Arabidopsis SPL gene family,
MFCSIQCVARHLFPLHVREIKKATRAI SEQ ID NO: 1 (10.57/3824) thaliana
involved in KKGKTL AtmiPEP156a2 miR156a Arabidopsis development of
the MRRQTSVPFACKRDKESDKSHKER SEQ ID NO: 2 thaliana stem and
flowering AtmiPEP156a3 miR156a Arabidopsis
MVMFFLDLDKNPRFDLLKGLKWNLF SEQ ID NO: 3 thaliana SSHISPSLPPSL
AtmiPEP156c1 miR156c Arabidopsis MKDNFPLLLRL SEQ ID NO: 4
(8.5/1359) thaliana AtmiPEP156c2 miR156c Arabidopsis MSDD SEQ ID
NO: 5 thaliana AtmiPEP156e1 miR156e Arabidopsis MIYINKYGSISAVEDD
SEQ ID NO: 6 (4.03/1818) thaliana AtmiPEP156f1 miR156f Arabidopsis
MSQR SEQ ID NO: 7 (9.5/520) thaliana AlmiPEP159a miR159a
Arabidopsis MYB gene family MTCPLLSLSFLLSKYI SEQ ID NO: 8 lyrata
involved in AtmiPEP159a1 miR159a Arabidopsis germination and
MTWPLLSLSFLLSKYV SEQ ID NO: 9 (8.34/1898) thaliana flowering
CrmiPEP159a miR159a Capsella MTCTLSALSLSLNMFRVN SEQ ID NO: 10
rubella AtmiPEP159b1 miR159b Arabidopsis MFYLS SEQ ID NO: 11
(5.27/659) thaliana AtmiPEP159b2 miR159b Arabidopsis
MVNTSSFFISSFILPLVLSESNCLLFRTI SEQ ID NO: 12 thaliana YKFSMVLY
AtmiPEP160a1 miR160a Arabidopsis ARF gene family,
MFCLLIPIFSFVFSPNRHLRLQEQ SEQ ID NO: 13 (8.02/2936) thaliana
involved in AtmiPEP160b1 miR160b Arabidopsis germination MFSPQ SEQ
ID NO: 14 (5.28/608) thaliana development and AtmiPEP160b2 miR160b
Arabidopsis flowering MKYIHILILFKSRSTYKLSTNHI SEQ ID NO: 15
thaliana AtmiPEP161 miR161 Arabidopsis PPR gene family MKIPLFLPKL
SEQ ID NO: 16 (10/1199) thaliana DCL1 gene, AtmiPEP162a1 miR162a
Arabidopsis involved MVSGQEDSWLKLSSLCFLFLSLLDSLI SEQ ID NO: 17
(4.03/3045) thaliana in development AtmiPEP162b1 miR162b
Arabidopsis MFLLIFLRLIMICVCSSTDFLRSVNYFC SEQ ID NO: 18 (5.71/4114)
thaliana LFIYDL AtmiPEP163-1 miR163 Arabidopsis SAMT gene family,
MSTTQEHRS SEQ ID NO: 19 (6.5/1076) thaliana involved in the
AtmiPEP163-2 miR163 Arabidopsis production of
MILKCWSSRFLRVSPYQNAHSLSLG SEQ ID NO: 20 thaliana secondary
metabolites AlmiPEP164a1 miR164a Arabidopsis NAC gene family,
MPLAVIRQGIVWP SEQ ID NO: 21 lyrata involved in root, AlmiPEP164a2
miR164a Arabidopsis foliar and floral MPSWHDMVLLPYVKHTHANTRHIT SEQ
ID NO: 22 lyrata devlopment AlmiPEP164a3 miR164a Arabidopsis
MTWFFCLT SEQ ID NO: 23 lyrata AtmiPEP164a1 miR164a Arabidopsis
MPSWHGMVLLPYVKHTHASTHTHTH SEQ ID NO: 24 (7.05/4256) thaliana
NIYGCACELVFH AtmiPEP164a2 miR164a Arabidopsis
MAWYGSFALRKTHSRQHTHTHT SEQ ID NO: 25 thaliana AtmiPEP164a3 miR164a
Arabidopsis MVWFFCLT SEQ ID NO: 26 thaliana BrmiPEP164a1 miR164a
Brassica rapa MMIILWK SEQ ID NO: 27 BrmiPEP164a2 miR164a Brassica
rapa MLWAKLVSFSTLHSLVFLLSPSFA SEQ ID NO: 28 BrmiPEP164a3 miR164a
Brassica rapa MPSWHGIVILPFVKHTHANIHYSYSC SEQ ID NO: 29 VCI
CpmiPEP164a1 miR164a Carica papaya MIACHPYLPFPLFLSLTFYSIFFSPSPPS
SEQ ID NO: 30 PSLPL CpmiPEP164a2 miR164a Carica papaya
MPSLLAFSPFPFSNILLNLLLPLPPFPLS SEQ ID NO: 31 CrmiPEP164a1 miR164a
Capsella rubella MELKGLRTWQLLDKV SEQ ID NO: 32 CrmiPEP164a2 miR164a
Capsella rubella MPSWHGMACFYCLT SEQ ID NO: 33 CrmiPEP164a3 miR164a
Capsella rubella MAWHGMFLLPYVKHTHANTYSLYM SEQ ID NO: 34
GrmiPEP164a1 miR164a Gossypium MMRSRILKFQYRFGMGIGGRKQLKN SEQ ID NO:
35 raimondii QLCQIQGRIS GrmiPEP164a2 miR164a Gossypium MSNSRSYQLK
SEQ ID NO: 36 raimondii GrmiPEP164a3 miR164a Gossypium
MNEDLEISTRKRTPQLC SEQ ID NO: 37 raimondii MtmiPEP164a1 miR164a
Medicago MPKFDIFFYIFV SEQ ID NO: 38 truncatula MtmiPEP164a2 miR164a
Medicago MSYISLSPKLLPINTKPFPWLVQFNFY SEQ ID NO: 39 truncatula
FSSNTKCNKLHFLGEKLLVGEAGHVQ ILFLIHSLIMHINIFCTCSPSPTRLPHPSL
OsmiPEP164a1 miR164a Oryza sativa MQTHSNTPQSTYSLSLSLSE SEQ ID NO:
40 OsmiPEP164a2 miR164a Oryza sativa MCVCDINMHSMLMLL SEQ ID NO: 41
AlmiPEP165a miR165a Arabidopsis HD-ZIPIII gene
MRIKLFQLRGMLSGSRILYIYTCVC SEQ ID NO: 42 lyrata family, involved
AtmiPEP165a miR165a Arabidopsis in vascular, root,
MRVKLFQLRGMLSGSRIL SEQ ID NO: 43 (12.3/2105) thaliana foliar and
floral BcmiPEP165a miR165a Brassica development, and
MRMKLFQLRGMLSGSRILYIHKYVY SEQ ID NO: 44 carinata nodulation
MLIQVFDHICI BjmiPEP165a miR165a Brassica MRMKLFQLRGMLSGSRILYIHKYVYI
SEQ ID NO: 45 juncea C BnmiPEP165a miR165a Brassica napus
MRMKLFQLRGMLSGSRILYIHKYVY SEQ ID NO: 46 MIIQVFDHICI BomiPEP165a
miR165a Brassica MRMKLFQLRGMLSGSRILYIHKYVY SEQ ID NO: 47 oleracea
MLIQVFDHICI BrmiPEP165a miR165a Brassica rapa
MRMKLFQLRGMLSGSRILYIHKYVYIC SEQ ID NO: 48 AtmiPEP166a miR166a
Arabidopsis MLDLFRSNNRIEPSDFRFD SEQ ID NO: 49 (4.68/2372) thaliana
AtmiPEP166b miR166b Arabidopsis MRDR SEQ ID NO: 50 (9.35/576)
thaliana AtmiPEP167a miR167a Arabidopsis ARF gene family,
MNRKISLSLS SEQ ID NO: 51 (11/1148) thaliana involved in root
AtmiPEP167b1 miR167b Arabidopsis and floral MMGCFVGF SEQ ID NO: 52
(5.27/891) thaliana development AtmiPEP167b2 miR167b Arabidopsis
MQEETYEG SEQ ID NO: 53 thaliana AtmiPEP169c1 miR169c Arabidopsis
gene family of MPHTNLKDLFIFSPNVFFSFAIYLHNS SEQ ID NO: 54 (9.3/7110)
thaliana CCAAT-bing factor, WNKNYIHKRENFHNTSFALIFFFSSIM involved in
SINYG AtmiPEP169c2 miR169c Arabidopsis nodulation,
MFFFRLLFISTILGTKTTFTNERIFTTPL SEQ ID NO: 55 thaliana drought
LLSFFFFRPL AtmiPEP1691 miR1691 Arabidopsis resistance, MRHKES SEQ
ID NO: 56 (8.52/786) thaliana resistance to nitrogen deficiency
AtmiPEP171a1 miR171a Arabidopsis GRAS gene family,
MNLLKKERQRRRQRSIGSHCIASLVL SEQ ID NO: 57 (11.05/4057) thaliana
involved in floral KDGYMKKI AtmiPEP171b miR171b Arabidopsis foliar,
and root MVLSGKLTF SEQ ID NO: 58 (8.5/995) thaliana development
MtmiPEP171b1 miR171b Medicago my corrhization MLLHRLSKFCKIERDIVYIS
SEQ ID NO: 59 truncatula nodulation MtmiPEP171b2 miR171b Medicago
development, MKIEE SEQ ID NO: 60 truncatula ZmmiPEP171b miR171b Zea
mays MHLPSTPSRPPPQHTSLSFLGKEMTKG SEQ ID NO: 61 TTTACFG AtmiPEP171c1
miR171c Arabidopsis MLSLSHFHIC SEQ ID NO: 62 (6.68/1187) thaliana
MtmiPEP171e miR171e Medicago MMVFGKPKKAMLVRFNPKTDLHV SEQ ID NO: 63
truncatula MtmiPEP171h miR171h Medicago MASAAKVYMA SEQ ID NO: 64
truncatula AtmiPEP172a1 miR172a Arabidopsis AP2 gene family, MASKIW
SEQ ID NO: 65 (8.5/734) thaliana involved in floral AtmiPEP172a3
miR172a Arabidopsis devolpment MVRFQLSIRD SEQ ID NO: 66 thaliana
AtmiPEP172b1 miR172b Arabidopsis MCTYYYLINKYF SEQ ID NO: 67
(7.9/1621) thaliana AtmiPEP172c1 miR172c Arabidopsis MFPAKWCRLES
SEQ ID NO: 68 (7.98/1367) thaliana AtmiPEP172e1 miR172e Arabidopsis
MGSLSLFKSQLEILMLLLSLSK SEQ ID NO: 69 (8.35/2452) thaliana
AtmiPEP172e2 miR172e Arabidopsis MSVYIHVPISLNCFSPKSSC SEQ ID NO: 70
thaliana AtmiPEP172e3 miR172e Arabidopsis MGVPNFRPRNR SEQ ID NO: 71
thaliana AcmiPEP319a1 miR319a Arabidopsis TCP gene family
MRSRVSFFFFKIMLFRLLGYRSM SEQ ID NO: 72 cebennensis involved in
floral AcmiPEP319a2 miR319a Arabidopsis and foliar
MHTYIHTISNISSIFFCSKRSFSPFTYIRI SEQ ID NO: 73 cebennensis devlopment
IVVIDPFRIALTFR AhmiPEP319a miR319a Arabidopsis
MRSRVSLFLSFSSNFAAYSPRS SEQ ID NO: 74 halleri AlmiPEP319a miR319a
Arabidopsis MHTYIPSSSFPISNISSVFFCYKRSFSPY SEQ ID NO: 75 lyrata
TYIRIIVVIDPFRIALTFR AtmiPEP319a1 miR319a Arabidopsis
MNIHTYHHLLFPSLVFHQSSDVPNALS SEQ ID NO: 76 (6.56/5917) thaliana
LHIHTYEYIIVVIDPFRITLAFR AtmiPEP319a2 miR319a Arabidopsis
MFQTLYLFIYIHTNILLLS SEQ ID NO: 77 thaliana BrmiPEP319a miR319a
Brassica rapa MFKLYFSAILSTQYMHTYHHRIALIFL SEQ ID NO: 78
SILYPSTNYLMSPILNPT CpmiPEP319a miR319a Carica papaya
MKIKLGFSLIKIIILLDKNS SEQ ID NO: 79 CrmiPEP319a miR319a Capsella
rubella MHPHTYIHIP SSSFLISSFCL SEQ ID NO: 80 EgmiPEP319a miR319a
Eucalyptus MKHIQRWRYGETSGRQGDWKRLEIK SEQ ID NO: 81 grandis
VHSNPSLKVKKNTNNFSSSL GrmiPEP319a miR319a Gossypium
MIHFNLSQWRAIMANFHLTYSFLFGC SEQ ID NO: 82 raimondii VL MtmiPEP319a
miR319a Medicago MHVYLELFMVIKGLGFLLLVK SEQ ID NO: 83 truncatula
OsmiPEP319a miR319a Oryza sativa MEMIQRPCLILKFFFKLSTLYIP SEQ ID NO:
84 PpmiPEP319a miR319a Physcomitrella MFHRRRSSVLLPPFGQTQPNPRCLPDL
SEQ ID NO: 85 patens RFPSCFTPCTA ThmiPEP319a1 miR319a Thellungiella
MTICKVSKACFYAGKIENSRLIKKIGIP SEQ ID NO: 86 halophila KREGAPFSPIRENQ
ThmiPEP319a2 miR319a Thellungiella MEIQIKKKNLYIMNTQKLPNLYIYIYK SEQ
ID NO: 87 halophila YVFIKLMVVE AtmiPEP319b1 miR319b Arabidopsis
MVPQINLWSSRVILKIRIDSSTHREED SEQ ID NO: 88 (8.04/5120) thaliana
HCIQNHKHGLSFIFSF AtmiPEP394a1 miR394a Arabidopsis F-box gene
family, MSLQFYERVSFKNTVK SEQ ID NO: 89 (9.7/1977) thaliana involved
in foliar development and drought resistance AtmiPEP395c1 miR395c
Arabidopsis Family of the APS MTEQEEESQMST SEQ ID NO: 90
(3.58/1429) thaliana and AST genes, AtmiPEP395e1 miR395e
Arabidopsis involved in MYLQYIDNVISIYSNNRRVGRMFSRV SEQ ID NO: 91
(9.98/4700) thaliana germination and PLSTSLEIQFFIK sulphur
metabolism AtmiPEP397b1 miR397b Arabidopsis Family of the
MSKEIFFSPGFE SEQ ID NO: 92 (4.53/1418) thaliana genes of laccases,
involved in copper metabolism, their overexpression improves growth
AtmiPEP398c1 miR398c Arabidopsis CSD gene family,
MRTHEQSTAITTLRHCYSSRFMCSQV SEQ ID NO: 93 thaliana involved in
copper TPAELFLYRPCFINAVAR metabolism, its overexpression improves
growth AtmiPEP399b miR399b Arabidopsis PHO2 gene family, MKRNM SEQ
ID NO: 94 (11/678) thaliana involved in AtmiPEP399d1 miR399d
Arabidopsis phosphorus MQCEI SEQ ID NO: 95 (4/622) thaliana
metabolism AtmiPEP403 miR403 Arabidopsis AGO gene family MFCA SEQ
ID NO: 96 (5.27470) thaliana AtmiPEP447a1 miR447a Arabidopsis
Family of the MVMAHH SEQ ID NO: 97 (6.69/724) thaliana genes of
AtmiPEP447a2 miR447a Arabidopsis phosphoglycerate
MMKPRWNCSLYGITEWTNNQNQKSK SEQ ID NO: 98 thaliana kinase
RKGRRKTQIWRIGDRLDTVECITLML SAY AtmiPEP447b1 miR447b Arabidopsis
MLLIIVELVL SEQ ID NO: 99 (4/1155) thaliana AtmiPEP447b2 miR447b
Arabidopsis MLCFNFRCVRRFAE SEQ ID NO: 100 thaliana AtmiPEP447c
miR447c Arabidopsis MYTYQLDNSFSWFLCTRFCLYRYFLF SEQ ID NO: 101
thaliana NFRCFRRFSE DmmiPEP1a miR1 Drosophila Muscular
MWREVCAQKSQTKRRNFITGNQRRN SEQ ID NO: 102 melanogaster
differentiation KTKANRKAETKQQKVYEFFVQARER CKTRKKHEKKTLKKTKKIQNRYRAV
SENEWGKGFPSHI DmmiPEP1b miR1 Drosophila Muscular
MRTKKSNKKAQFYYGQPTTKQNKSQ SEQ ID NO: 103 melanogaster
differentiation PKSRNKAAKSL DmmiPEP8 miR8 Drosophila Growth
MEPGFVFVLFPTHLSTQHTQREKSILV SEQ ID NO: 104 melanogaster
MGLNLQSAKQSDKQNSKERKKNTQI NSQRIPYRQGGQCSKVLSP HsmiPEP155 miR155
Homo sapiens inflammation MEMALMVAQTRKGKSVV SEQ ID NO: 355
AtmiPEP157c miR157c Arabidopsis SPL gene family, MMLHITHRFESDVGC
SEQ ID NO: 375 (5.95/1776) thaliana involved in AtmiPEP157d miR157d
Arabidopsis development of the MLYV SEQ ID NO: 376 (5.27/524)
thaliana stem and flowering AtmiPEP160c miR160c Arabidopsis ARF
gene family, MFMRRGLVYNNIYI SEQ ID NO: 377 (9.98/1790) thaliana
involved in germination, development and flowering AtmiPEP164b
miR164b Arabidopsis NAC gene family, MMKVCDEQDGEAGHVHY SEQ ID NO:
378 (4.72/1949) thaliana involved in root, foliar and floral
development AtmiPEP166c miR166c Arabidopsis HD-ZIPIII gene
MKKRITRINLEEQIKKTLDDSRTRLHS SEQ ID NO: 379 (10.42/3407) thaliana
family, involved P in vascular, root, foliar and floral development
and nodulation AtmiPEP166d miR166d Arabidopsis MKKIGSIDSF SEQ ID
NO: 380 (8.35/1125) thaliana AtmiPEP169a miR169a Arabidopsis Gene
family of MTCRFK SEQ ID NO: 381 (9.5/784) thaliana CCAAT-bing
factor, AtmiPEP169h1 miR169h Arabidopsis MVT (5.28/349) thaliana
involved in AtmiPEP169h2 miR169h Arabidopsis nodulation,
MKNENLCGSQG SEQ ID NO: 382 thaliana drought AtmiPEP169n miR169n
Arabidopsis resistance, MKCMMKKRGLTWRKASCLVAKDDL SEQ ID NO: 383
(8.96/5315) thaliana resistance to PDLFRLHDSISNSCILDYYTF nitrogen
deficiency AtmiPEP170 miR170 Arabidopsis GRAS gene family, MFPRESL
SEQ ID NO: 384 (5.75/879) thaliana involved in floral, foliar, and
root development, mycorrhization, nodulation AtmiPEP396a miR396a
Arabidopsis Family of the GRF MTLSVFFHSFLELQNFFRFFFFSFDISY SEQ ID
NO: 385 (5.3/3636) thaliana genes involved in A root development
and cellular proliferation, mycorrhization AtmiPEP399c miR399c
Arabidopsis PHO2 gene family, MSLAKGELPCHCFRLNTVYNRFC SEQ ID NO:
386 (8.66/2703) thaliana involved in phosphorus metabolism
MtmiPEP169d miR169d Medicago Family NF-YA MVKESFMERLKVR SEQ ID NO:
424 tuncatula genes (or HAP2 genes)
TABLE-US-00002 TABLE 2 List of the miORFs miPEP Organism Sequence
of the miORF SEQ ID AtmiPEP156a1 Arabidopsis thaliana
ATGTTCTGTTCAATTCAATGCGTCGCCAGACATCTGTTCCCTTTGC SEQ ID NO: 105
ATGTAAGAGAGATAAAGAAAGCGACAAGAGCCATAAAGAAAGG TAA AtmiPEP156a2
Arabidopsis thaliana ATGCGTCGCCAGACATCTGTTCCCTTTGCATGTAAGAGAGATAAA
SEQ ID NO: 106 GAAAGCGACAAGAGCCATAAAGAAAGGTAA AtmiPEP156a3
Arabidopsis thaliana ATGGTTATGTTTTTTCTCGATTTAGACAAAAACCCTAGATTTGATC
SEQ ID NO: 107 TTCTAAAGGGTCTCAAATGGAATCTCTTCTCTTCTCATATCTCTCC
CTCTCTCCCTCCCTCTCTTTGA AtmiPEP156c1 Arabidopsis thaliana
ATGAAGGACAACTTTCCTCTTCTCCTTCGGTTATAA SEQ ID NO: 108 AtmiPEP156c2
Arabidopsis thaliana ATGAGTGATGACTGA SEQ ID NO: 109 AtmiPEP156e1
Arabidopsis thaliana ATGATATATATAAATAAATATGGGTCGATATCGGCTGTGGAGGAC
SEQ ID NO: 110 GACTAG AtmiPEP156f1 Arabidopsis thaliana
ATGAGCCAAAGATAA SEQ ID NO: 111 AlmiPEP159a Arabidopsis lyrata
ATGACGTGTCCTCTTCTCTCTCTCTCTTTCCTTCTCTCTAAGTATAT SEQ ID NO: 112 TTAG
AtmiPEP159a1 Arabidopsis thaliana
ATGACGTGGCCTCTTCTCTCTCTCTCTTTCCTTCTCTCTAAGTATGT SEQ ID NO: 113 TTAG
CrmiPEP159a Capsella rubella
ATGACGTGTACTCTCTCTGCTCTATCTCTCTCTCTAAATATGTTTA SEQ ID NO: 114
GGGTTAA AtmiPEP159b1 Arabidopsis thaliana ATGTTTTATCTTTCATAA SEQ ID
NO: 115 AtmiPEP159b2 Arabidopsis thaliana
ATGGTTAATACTAGTAGCTTTTTCATTTCAAGTTTTATCCTTCCAT SEQ ID NO: 116
TGGTTCTTTCTGAGTCAAATTGTCTCCTGTTTCGAACCATATATAA
GTTTTCAATGGTTTTGTATTAA AtmiPEP160a1 Arabidopsis thaliana
ATGTTTTGTTTGTTGATTCCCATCTTCTCTTTTGTCTTTTCACCAAA SEQ ID NO: 117
TCGTCATTTAAGGCTTCAAGAACAGTAA AtmiPEP160b1 Arabidopsis thaliana
ATGTTTTCCCCTCAATGA SEQ ID NO: 118 AtmiPEP160b2 Arabidopsis thaliana
ATGAAATACATACACATTTTGATTTTATTTAAATCAAGATCGACG SEQ ID NO: 119
TATAAGCTATCCACCAATCATATTTAA AtmiPEP161 Arabidopsis thaliana
ATGAAAATTCCATTGTTTCTGCCGAAGCTTTGA SEQ ID NO: 120 AtmiPEP162a1
Arabidopsis thaliana ATGGTATCTGGTCAAGAAGATTCCTGGTTAAAACTTTCATCTCTCT
SEQ ID NO: 121 GTTTCCTTTTTCTTTCTTTGTTGGATTCATTAATTTGA AtmiPEP162b1
Arabidopsis thaliana
ATGTTTCTTTTAATCTTTTTGAGATTAATAATGATTTGTGTTTGTTC SEQ ID NO: 122
ATCAACCGATTTTCTCAGATCTGTCAATTATTTTTGTTTATTTATTT ATGATTTATGA
AtmiPEP163-1 Arabidopsis thaliana ATGTCCACTACTCAAGAGCATAGGTCTTGA
SEQ ID NO: 123 AtmiPEP163-2 Arabidopsis thaliana
ATGATACTAAAGTGCTGGAGTTCCCGGTTCCTGAGAGTGAGTCCA SEQ ID NO: 124
TATCAAAATGCGCATTCGTTATCACTTGGTTGA AlmiPEP164a1 Arabidopsis lyrata
ATGCCCTTAGCAGTTATTAGACAAGGGATTGTTTGGCCCTAG SEQ ID NO: 125
AlmiPEP164a2 Arabidopsis lyrata
ATGCCATCATGGCATGACATGGTTCTTTTGCCTTACGTAAAACAC SEQ ID NO: 126
ACTCACGCCAACACACGCCACATAACATAA AlmiPEP164a3 Arabidopsis lyrata
ATGACATGGTTCTTTTGCCTTACGTAA SEQ ID NO: 127 AtmiPEP164a1 Arabidopsis
thaliana ATGCCATCATGGCATGGTATGGTTCTTTTGCCTTACGTAAAACAC SEQ ID NO:
128 ACTCACGCCAGCACACACACACACACACATAACATATACGGATG
TGCGTGTGAGCTAGTCTTCCATTAA AtmiPEP164a2 Arabidopsis thaliana
ATGGCATGGTATGGTTCTTTTGCCTTACGTAAAACACACTCACGC SEQ ID NO: 129
CAGCACACACACACACACACATAA AtmiPEP164a3 Arabidopsis thaliana
ATGGTATGGTTCTTTTGCCTTACGTAA SEQ ID NO: 130 BrmiPEP164a1 Brassica
rapa ATGATGATAATTTTGTGGAAATAA SEQ ID NO: 131 BrmiPEP164a2 Brassica
rapa ATGCTTTGGGCCAAGCTAGTTTCTTTTAGCACTCTTCACTCACTAG SEQ ID NO: 132
TTTTTCTTCTCAGCCCTTCTTTTGCGTGA BrmiPEP164a3 Brassica rapa
ATGCCATCATGGCATGGCATTGTCATTTTGCCTTTCGTAAAACAC SEQ ID NO: 133
ACTCACGCCAACATACATTATTCATATTCATGTGTATGTATATGA
ATGCCATCATGGCATATGCCATCATGGCAT CpmiPEP164a1 Carica papaya
ATGATTGCATGCCATCCCTACTTGCCTTTTCCCCTTTTCCTTTCTCT SEQ ID NO: 134
AACATTTTACTCAATCTTCTTCTCCCCCTCCCCCCCTTCCCCCTCTC TGCCATTATAA
CpmiPEP164a2 Carica papaya
ATGCCATCCCTACTTGCCTTTTCCCCTTTTCCTTTCTCTAACATTTT SEQ ID NO: 135
ACTCAATCTTCTTCTCCCCCTCCCCCCCTTCCCCCTCTCTGCCATTA
TAACCATAATTAAACCTCTCTCCCTCTCTCTCCCTCTCTCTCTCTCT
CTCTCTGGGTTCTCAGTATAA CrmiPEP164a1 Capsella rubella
ATGGAATTAAAAGGTTTGAGAACTTGGCAGTTATTATTAGACAAGGT SEQ ID NO: 136 ATAG
CrmiPEP164a2 Capsella rubella
ATGCCATCATGGCATGGCATGGCATGTTTCTATTGCCTTACGTAA SEQ ID NO: 137
CrmiPEP164a3 Capsella rubella
ATGGCATGGCATGTTTCTATTGCCTTACGTAAAACACACTCACGC SEQ ID NO: 138
CAACACATACTCACTATACATGTAAATAAGTATGTGCGCGTGTGA GrmiPEP164a1
Gossypium raimondii ATGATGAGATCAAGAATTTTAAAGTTTCAATATAGATTTGGCATG
SEQ ID NO: 139 GGTATTGGCGGCAGAAAGCAATTAAAAAACCAGTTATGTCAAAT
TCAAGGTCGTATCAGTTAA GrmiPEP164a2 Gossypium raimondii
ATGTCAAATTCAAGGTCGTATCAGTTAAAATGA SEQ ID NO: 140 GrmiPEP164a3
Gossypium raimondii ATGAATGAAGATTTAGAAATTTCAACAAGGAAGAGGACCCCACA
SEQ ID NO: 141 GCTTTGTTAA MtmiPEP164a1 Medicago truncatula
ATGCCCAAATTTGATATTTTTTTTTATATATTTGTATAG SEQ ID NO: 142 MtmiPEP164a2
Medicago truncatula ATGTCATATATCTCTCTCTCTCCTAAGTTGCTACCTATAAATACTA
SEQ ID NO: 143 AGCCTTTCCCTTGGTTGGTTCAATTCAACTTCTACTTCTCATCAAA
CACAAAGTGCAATAAGCTTCATTTCCTGGGTGAGAAGCTCCTTGT
TGGAGAAGCAGGGCACGTGCAAATCCTCTTTCTGATTCATTCTCT
CATAATGCATATCAATATCTTTTGCACGTGCTCCCCTTCTCCAACT AGG OsmiPEP164a1
Oryza sativa ATGCAAACCCACTCCAACACTCCACAATCCACATACTCTCTCTCT SEQ ID
NO: 144 CTCTCTCTCTCTGAGTAG OsmiPEP164a2 Oryza sativa
ATGTGTGTGTGTGATATCAATATGCATTCGATGTTGATGCTACTGT SEQ ID NO: 145 AG
AlmiPEP165a Arabidopsis lyrata
ATGAGAATTAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 146
AGGATATTATACATATATACATGTGTATGTTGA AtmiPEP165a Arabidopsis thaliana
ATGAGGGTTAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 147
AGGATATTATAG BcmiPEP165a Brassica carinata
ATGAGAATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 148
AGGATATTATATATACACAAATACGTATATATGTTAATACAAGTG TTTGATCATATATGTATATAG
BjmiPEP165a Brassica juncea
ATGAGAATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 149
AGGATATTATATATACACAAATATGTATATATATGTTAA BnmiPEP165a Brassica napus
ATGAGAATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 150
AGGATATTATATATACACAAATACGTATATATGATAATACAAGTG TTTGATCATATATGTATATAG
BomiPEP165a Brassica oleracea
ATGAGAATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 151
AGGATATTATATATACACAAGTACGTATATATGTTAATACAAGTG TTTGATCATATATGTATATAG
BrmiPEP165a Brassica rapa
ATGAGAATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCG SEQ ID NO: 152
AGGATATTATATATACACAAATATGTATATATATGTTAA AtmiPEP166a Arabidopsis
thaliana ATGTTGGATCTCTTTCGATCTAACAATCGAATTGAACCTTCAGATT SEQ ID NO:
153 TCAGATTTGATTAG AtmiPEP166b Arabidopsis thaliana ATGAGAGATAGATAA
SEQ ID NO: 154 AtmiPEP167a Arabidopsis thaliana
ATGAACAGAAAAATCTCTCTTTCTCTTTCTTGA SEQ ID NO: 155 AtmiPEP167b1
Arabidopsis thaliana ATGATGGGTTGTTTTGTGGGATTTTAA SEQ ID NO: 156
AtmiPEP167b2 Arabidopsis thaliana ATGCAGGAGGAAACATATGAGGGGTGA SEQ
ID NO: 157 AtmiPEP169c1 Arabidopsis thaliana
ATGCCACATACAAACTTGAAAGATCTCTTCATCTTTTCTCCAAATG SEQ ID NO: 158
TTTTTTTTTCGTTTGCTATTTATCTCCACAATTCTTGGAACAAAAA
CTACATTCACAAACGAGAGAATTTTCACAACACCTCTTTTGCTCTC
ATTTTTTTTTTTTCGTCCATTATGAGTATTAATTATGGTTAG AtmiPEP169c2 Arabidopsis
thaliana ATGTTTTTTTTTCGTTTGCTATTTATCTCCACAATTCTTGGAACAA SEQ ID NO:
159 AAACTACATTCACAAACGAGAGAATTTTCACAACACCTCTTTTGC
TCTCATTTTTTTTTTTTCGTCCATTATGA AtmiPEP16911 Arabidopsis thaliana
ATGAGACATAAAGAGAGTTAA SEQ ID NO: 160 AtmiPEP171a1 Arabidopsis
thaliana ATGAACCTCCTCAAGAAGGAAAGACAGAGGAGGAGACAAAGAA SEQ ID NO: 161
GTATAGGTTCACATTGCATAGCCAGTTTAGTTTTGAAGGATGGAT ATATGAAAAAAATATGA
AtmiPEP171b Arabidopsis thaliana ATGGTTCTCTCCGGTAAATTAACATTTTAG SEQ
ID NO: 162 MtmiPEP171b1 Medicago truncatula
ATGCTTCTTCATAGGCTCTCCAAATTTTGCAAAATTGAAAGAGAC SEQ ID NO: 163
ATAGTATATATATCTTAG MtmiPEP171b2 Medicago truncatula
ATGAAGATTGAAGAGTAA SEQ ID NO: 164 ZmmiPEP171b Zea mays
ATGCATCTGCCTTCAACTCCCTCTCGCCCCCCACCCCAACACACAT SEQ ID NO: 165
CTCTCTCTTTTCTAGGGAAGGAAATGACGAAGGGGACGACGACG GCATGCTTCGGCTAG
AtmiPEP171c1 Arabidopsis thaliana ATGTTGTCTCTTTCTCATTTTCATATCTGCTAA
SEQ ID NO: 166 MtmiPEP171e Medicago truncatula
ATGATGGTGTTTGGGAAGCCGAAAAAAGCGATGTTGGTGAGGTT SEQ ID NO: 167
CAATCCGAAGACGGATTTACATGTATAG MtmiPEP171h Medicago truncatula
ATGGCTTCAGCTGCAAAAGTATACATGGCGTGA SEQ ID
NO: 168 AtmiPEP172a1 Arabidopsis thaliana ATGGCTTCCAAGATCTGGTAA SEQ
ID NO: 169 AtmiPEP172a3 Arabidopsis thaliana
ATGGTTAGGTTCCAACTAAGTATACGAGATTAA SEQ ID NO: 170 AtmiPEP172b1
Arabidopsis thaliana ATGTGTACGTACTATTATCTCATAAATAAATATTTTTAA SEQ ID
NO: 171 AtmiPEP172c1 Arabidopsis thaliana
ATGTTTCCAGCAAAATGGTGCCGTCTTGAGTCTTGA SEQ ID NO: 172 AtmiPEP172e1
Arabidopsis thaliana ATGGGATCTCTCTCTTTATTTAAAAGTCAATTAGAGATCTTGATGC
SEQ ID NO: 173 TACTTCTGTCCCTTTCCAAGTGA AtmiPEP172e2 Arabidopsis
thaliana ATGAGTGTATATATTCATGTACCTATCTCTCTCAATTGCTTCTCAC SEQ ID NO:
174 CAAAATCATCTTGCTGA AtmiPEP172e3 Arabidopsis thaliana
ATGGGAGTTCCCAACTTTAGACCTCGAAACCGATAA SEQ ID NO: 175 AcmiPEP319a1
Arabidopsis cebennensis
TAGAGATCTAGGGTTTCTTTCTTTTTCTTCAAAATCATGCTTTTTC SEQ ID NO: 176
GCTTGCTAGGTTATAGATCCATGTAA AcmiPEP319a2 Arabidopsis cebennensis
ATGCATACATACATACATACCATCTCTAATATTTCATCAATCTTCT SEQ ID NO: 177
TTTGTTCCAAACGCCTTTCTCTCCATTTACATACATACGAATCATT
GTTGTCATAGATCCGTTTAGAATTGCTTTAACTTTTAGATGA AhmiPEP319a Arabidopsis
halleri ATGAGATCTAGGGTTTCTTTGTTTCTTTCGTTTTCTTCAAATTTTGC SEQ ID NO:
178 TGCATATTCTCCAAGATCATGA AlmiPEP319a Arabidopsis lyrata
ATGCATACATACATACCATCATCATCTTTTCCCATCTCTAATATTT SEQ ID NO: 179
CATCAGTCTTCTTTTGTTACAAACGCTCTTTCTCGCCATATACATA
CATAAGAATCATTGTTGTCATAGATCCGTTTAGAATTGCTTTAACT TTTAGATGA
AtmiPEP319a1 Arabidopsis thaliana
ATGAATATACATACATACCATCATCTTCTTTTCCCATCTCTAGTTT SEQ ID NO: 180
TTCATCAATCTTCTGATGTTCCAAACGCTCTATCTCTTCATATACA
TACATACGAATATATTATTGTTGTCATAGATCCATTTAGAATCACT TTAGCTTTTAGATGA
AtmiPEP319a2 Arabidopsis thaliana
ATGTTCCAAACGCTCTATCTCTTCATATACATACATACGAATATAT SEQ ID NO: 181
TATTGTTGTCATAG BrmiPEP319a Brassica rapa
ATGTTTAAGCTCTACTTCTCAGCAATTCTCTCCACCCAATACATGC SEQ ID NO: 182
ATACATACCATCATCGTATCGCTCTAATTTTTCTATCAATCTTGTA
TCCTTCCACAAATTATCTTATGTCTCCCATTTTAAATCCTACATAG CpmiPEP319a Carica
papaya ATGAAGATTAAATTAGGTTTTAGTCTTATTAAGATTATTATATTAC SEQ ID NO:
183 TAGACAAAAACAGTTAA CrmiPEP319a Capsella rubella
ATGCATCCACATACATACATACATATACCATCATCTTCTTTTCTCA SEQ ID NO: 184
TCTCTAGTTTTTGTTTATAA EgmiPEP319a Eucalyptus grandis
ATGAAGCATATTCAAAGGTGGAGATATGGGGAGACTTCCGGAAG SEQ ID NO: 185
GCAAGGGGATTGGAAAAGGCTCGAGATCAAAGTGCATAGCAACC
CTTCGCTAAAGGTGAAAAAGAATACGAATAACTTCAGTAGCTCAC TTTAA GrmiPEP319a
Gossypium raimondii ATGATCCATTTCAACCTGTCACAGTGGAGAGCAATTTGTATGGCT
SEQ ID NO: 186 AATTTCCATCTCACCTATTCTTTTCTGTTTGGGGTTCTCTAG
MtmiPEP319a Medicago truncatula
ATGCATGTATATCTTGAATTGTTTATGGTAATAAAGGGGTTAGGA SEQ ID NO: 187
TTTCTCCTTTTGGTGAAGTGA OsmiPEP319a Oryza sativa
ATGGAAATGATACAAAGGCCGTGTTTAATTTTAAAATTTTTTTTCA SEQ ID NO: 188
AACTTTCAACACTTTACATCCCATAA PpmiPEP319a Physcomitrella patens
ATGTTCCACCGTCGGAGATCCTCGGTGCTGCTACCCCCGTTCGGC SEQ ID NO: 189
CAAACCCAACCCAACCCTAGGTGTCTGCCGGACCTCCGCTTCCCC
TCCTGCTTCACCCCCTGCACCGCTTAA ThmiPEP319a1 Thellungiella halophila
ATGACGATATGTAAAGTAAGCAAGGCATGTTTTTATGCAGGGAA SEQ ID NO: 190
GATTGAAAATTCAAGATTAATCAAGAAAATTGGAATACCAAAAA
GAGAGGGAGCTCCCTTCAGTCCAATCAGAGAGAATCAATGA ThmiPEP319a2
Thellungiella halophila
ATGGAGATTCAAATTAAAAAGAAAAACTTATATATAATGAATAC SEQ ID NO: 191
ACAAAAGCTACCTAATCTGTATATATATATATATAAATATGTCTT
CATTAAATTAATGGTCGTGGAATAG AtmiPEP319b1 Arabidopsis thaliana
ATGGTACCTCAAATTAATCTATGGTCATCTAGGGTTATCTTGAAG SEQ ID NO: 192
ATTAGAATTGATTCTAGCACGCACAGAGAGGAAGATCATTGCATC
CAGAATCACAAACATG3GCCTATCTTTTATCTTTTCTTTTTGA AtmiPEP394a1
Arabidopsis thaliana ATGTCTCTCCAATTTTATGAGAGGGTTTCCTTCAAGAACACAGTA
SEQ ID NO: 193 AAATAG AtmiPEP395c1 Arabidopsis thaliana
ATGACAGAGCAAGAAGAAGAAAGTCAAATGTCCACATGA SEQ ID NO: 194 AtmiPEP395e1
Arabidopsis thaliana ATGTATCTACAATATATTGATAATGTAATATCTATATATTCAAAC
SEQ ID NO: 195 AATCGTCGTGTTGGTCGGATGTTTTCTAGAGTTCCTCTGAGCACTT
CATTGGAGATACAATTTTTTATAAAATAG AtmiPEP397b1 Arabidopsis thaliana
ATGAGCAAGGAGATATTTTTTTCCCCTGGGTTTGAATGA SEQ ID NO: 196 AtmiPEP398c1
Arabidopsis thaliana ATGAGAACACACGAGCAATCAACGGCTATAACGACGCTACGTCA
SEQ ID NO: 197 TTGTTACAGCTCTCGTTTCATGTGTTCTCAGGTCACCCCTGCTGAG
CTCTTTCTCTACCGTCCATGTTTTATCAACGCCGTGGCCCGTG AtmiPEP399b Arabidopsis
thaliana ATGAAGAGAAACATGTAA SEQ ID NO: 198 AtmiPEP399d1 Arabidopsis
thaliana ATGCAATGTGAAATATGA SEQ ID NO: 199 AtmiPEP403 Arabidopsis
thaliana ATGTTTTGTGCTTGA SEQ ID NO: 200 AtmiPEP447a1 Arabidopsis
thaliana ATGGTCATGGCTCATCATTAG SEQ ID NO: 201 AtmiPEP447a2
Arabidopsis thaliana ATGATGAAACCTCGATGGAACTGCTCTCTTTATGGAATCACGGAA
SEQ ID NO: 202 TGGACAAATAATCAAAATCAGAAATCGAAGCGAAAAGGGAGGA
GAAAAACGCAGATTTGGAGGATTGGGGACAGATTAGATACTGTT
GAATGCATCACTCTAATGCTATCAGCCTATTAA AtmiPEP447b1 Arabidopsis thaliana
ATGCTGCTTATCATCGTGGAGTTGGTTCTGTAA SEQ ID NO: 203 AtmiPEP447b2
Arabidopsis thaliana ATGCTTTGTTTCAATTTCAGGTGCGTTAGAAGGTTTGCAGAGTAG
SEQ ID NO: 204 AtmiPEP447c Arabidopsis thaliana
ATGTACACCTACCAGCTTGATAACTCTTTTTCGTGGTTTCTGTGTA SEQ ID NO: 205
CTCGTTTCTGTTTGTACAGATACTTCTTGTTCAATTTCAGATGCTTT AGAAGGTTTTCGGAG
dmmiPEP1a Drosophila melanogaster
ATGTGGCGCGAAGTATGCGCACAAAAAAGTCAAACAAAAAGGCG SEQ ID NO: 206
CAATTTTATTACGGGCAACCAACGACGAAACAAAACAAAAGCCA
ACCGAAAAGCAGAAACAAAGCAGCAAAAAGTTTATGAATTTTTT
GTGCAGGCGCGTGAAAGATGCAAAACGAGAAAAAAACATGAAA
AAAAAACATTAAAAAAAACAAAAAAAATCCAAAACAGATACCG
AGCTGTATCCGAAAACGAGTGGGGAAAGGGGTTTCCCAGTCACA TATAA DmmiPEP1b
Drosophila melanogaster
ATGCGCACAAAAAAGTCAAACAAAAAGGCGCAATTTTATTACGG SEQ ID NO: 207
GCAACCAACGACGAAACAAAACAAAAGCCAACCGAAAAGCAGA
AACAAAGCAGCAAAAAGTTTATGA DmmiPEP8 Drosophila melanogaster
ATGGAGCCTGGCTTTGTTTTTGTTTTATTTCCAACCCACTTGAGCA SEQ ID NO: 208
CACAGCACACACAGAGAGAAAAATCAATACTCGTTATGGGATTA
AATTTACAAAGCGCAAAGCAAAGCGACAAACAAAATTCAAAAGA
AAGAAAAAAAAACACTCAAATAAACTCACAAAGAATTCCTTATC
GCCAAGGGGGCCAATGTTCTAAGGTTCTTTCGCCTTGA HsmiPEP155 Homo sapiens
TGGAGATGGCTCTAATGGTGGCACAAACCAGGAAGGGGAAATCT SEQ ID NO: 356
GTGGTTTAA AtmiPEP157c Arabidopsis thaliana
ATGATGTTGCATATCACACATAGGTTTGAGAGTGATGTTGGTTGT SEQ ID NO: 387 TGA
AtmiPEP157d Arabidopsis thaliana ATGCTGTATGTATAG SEQ ID NO: 388
AtmiPEP160c Arabidopsis thaliana
ATGTTCATGCGTAGAGGTTTGGTATACAACAATATATACATATAA SEQ ID NO: 389
AtmiPEP164b Arabidopsis thaliana
ATGATGAAGGTGTGTGATGAGCAAGATGGAGAAGCAGGGCACGT SEQ ID NO: 390
GCATTACTAG AtmiPEP166c Arabidopsis thaliana
ATGAAGAAGAGAATCACTCGAATTAATTTGGAAGAACAAATTAA SEQ ID NO: 391
GAAAACCCTAGATGATTCTCGGACCAGGCTTCATTCCCCCTAA AtmiPEP166d Arabidopsis
thaliana ATGAAGAAGATCGGTAGtATTGATTCATTTTAA SEQ ID NO: 392
AtmiPEP169a Arabidopsis thaliana ATGACTTGCCGATTTAAATGA SEQ ID NO:
393 AtmiPEP169h1 Arabidopsis thaliana ATGGTGACATGA SEQ ID NO: 394
AtmiPEP169h2 Arabidopsis thaliana
ATGAAGAATGAGAACTTGTGTGGTAGCCAAGGATGA SEQ ID NO: 395 AtmiPEP169n
Arabidopsis thaliana ATGAAGTGTATGATGAAGAAGAGAGGTCTAACATGGCGGAAAGC
SEQ ID NO: 396 GTCATGTTTAGTAGCCAAGGATGACTTGCCTGATCTTTTTCGCCTC
CACGATTCAATTTCAAATTCATGCATTTTGGATTATTATACCTTTT AA AtmiPEP170
Arabidopsis thaliana ATGTTTCCGAGAGAGTCCCTCTGA SEQ ID NO: 397
AtmiPEP396a Arabidopsis thaliana
ATGACCCTCTCTGTATTCTTCCACAGCTTTCTTGAACTGCAAAACT SEQ ID NO: 398
TCTTCAGATTTTTTTTTTTTTCTTTTGATATCTCTTACGCATAA AtmiPEP399c
Arabidopsis thaliana ATGTCACTTGCCAAAGGAGAGTTGCCCTGTCACTGCTTCCGCTTA
SEQ ID NO: 399 AACACAGTCTATAACCGGTTCTGCTAA MtmiPEP169d Medicago
truncatula ATGGTCAAAGAGTCTTTCATGGAGAGGTTGAAAGTGAGATGA SEQ ID NO:
425
TABLE-US-00003 TABLE 3 List of the primary transcripts (pri-miRNAs)
miPEP Organism Sequence of the Pri-miRNA SEQ ID AtmiPEP156a1
Arabidopsis ATTCATTGTTCACTCTCAAATCTCAAGTTCATTGCCATTTTTAGGTCTCTC SEQ
ID NO: 209 AtmiPEP156a2 thaliana
TATAAATTCAAATGTTCTGTTCAATTCAATGCGTCGCCAGACATCTGTTC AtmiPEP156a3
CCTTTGCATGTAAGAGAGATAAAGAAAGCGACAAGAGCCATAAAGAAA
GGTAAGACTCTTTGAAATAGAGAGAGATAAGGTTTTCTCTTATCTTCTTC
TCATCAGATCTTTGTTTCTTTACCCTCTTTCTTTCTTTTTTTTGCTTTTTATG
GTTATGTTTTTTCTCGATTTAGACAAAAACCCTAGATTTGATCTTCTAAAG
GGTCTCAAATGGAATCTCTTCTCTTCTCATATCTCTCCCTCTCTCCCTCCC
TCTCTTTGATTCTTTGTCTTCTCCAGTTAAAACTCAGATCTAACACAAAGC
TTAAAAGATTCTCATCGTTTCTTGTTTTCTTTGTTTCATCTTGTAGATCTCT
GAAGTTGGACTAATTGTGAATGAAAGAGTTGGGACAAGAGAAACGCAA
AGAAACTGACAGAAGAGAGTGAGCACACAAAGGCAATTTGCATATCATT
GCACTTGCTTCTCTTGCGTGCTCACTGCTCTTTCTGTCAGATTCCGGTGCT GATCTCTTT
AtmiPEP156c1 Arabidopsis
CTCTGCCTTTAGTTCTTTCTTTTTTGGTAATATATTTATTTTTCGTTACGAT SEQ ID NO: 210
AtmiPEP156c2 thaliana
TTGGTCAAAACCCTAGATTTGTTTTCCAAAAGCATATCTGAAAATGAAGG
ACAACTTTCCTCTTCTCCTTCGGTTATAAATATTCTCTCCGGTTTTGCTTGT
TTAACCTAAAAGCCTCAGATCTAACTCCAACACCTTCAAAGTCTGCCTCC
TTTCCAATCTTCTTTCTTCTGTTCGATCTCTAATCTCAGAATTTGTGTCGGT
AAGGTAAAGGTGATAATGAGTGATGACTGATGAGGGAGTTTTGGGACAA
ATTTTAAGAGAAACGCATAGAAACTGACAGAAGAGAGTGAGCACACAA
AGGCACTTTGCATGTTCGATGCATTTGCTTCTCTTGCGTGCTCACTGCTCT
ATCTGTCAGATTCCGGCT AtmiPEP156e1 Arabidopsis
TCCCACATCCAAAGATAGAAAGATGTAAGGTCTAGAGTCTTGTTCTTAAT SEQ ID NO: 211
thaliana CCCCTAACAGAACAATGATATATATAAATAAATATGGGTCGATATCGGC
TGTGGAGGACGACTAGCTACGGTTTCGAGCCTGGTCACATGCGTAGAGT
GTGAAAGGTAATTAGGAGGTGACAGAAGAGAGTGAGCACACATGGTGG
TTTCTTGCATGCTTTTTTGATTAGGGTTTCATGCTTGAAGCTATGTGTGCT
TACTCTCTCTCTGTCACCCCT AtmiPEP156f1 Arabidopsis
TCCCACAGCCAATGAGCCAAAGATAAAGAAACACCTATCCTATAATAAT SEQ ID NO: 212
thaliana TTAGAGCAATATACCTCCATAATGGAACATCTATATATATAAAGGTATCC
GTATATCTCTATATATTATATTCATTGAGTTTAAAGTGGCTAGGGTTTATA
GATGTATGTGATATTAAGAGATATGAAACATATTTGTCGACGGTTTGAGT
GGTGAGGAATTGATGGTGACAGAAGAGAGTGAGCACACATGGTGGCTTT
CTTGCATATTTGAAGGTTCCATGCTTGAAGCTATGTGTGCTCACTCTCTAT
CCGTCACCCCCTTCTCTCCCTCTCCCTC AlmiPEP159a Arabidopsis
AAAAAATGACGTGTCCTCTTCTCTCTCTCTCTTTCCTTCTCTCTAAGTATA SEQ ID NO: 213
lyrata TTTAGGGTTAATTATTAGGGTTCTTTATCTCTTTCTTCAGTCTTTGAAGTTT
CTTCAATAGCTTTAATTGAAGTGATTTACCTCTCTGGGTGTTTTTAGTATA
TATATCATGTACATGATCGAATTTCTTTCTATCCAAGTTCTCATCAAACCT
TCTCATGTTTTGAAGAGTTAAAGGCTTTATAGTTTGCTTAGGTCAGATCC
ATAACATACTGTATTTGACAAGTTTCTTTGTCTCACGATAGATCTTGGTCT
GACCAAAATGATTTTCTCGAGAAAAAAAAAGATGGAAGTAGAGCTCCTT
GAAGTTCAAACGAGAGTTGAGCAGGGTAAAGAAAAGCTGCTAAGCTATG
GATCCCATAAGCCCTAATCCTTATAAAGAAAAAAAAGGATTTGGTTATAT
GGCTTGCATATCTCAGGAGCTTTAACTTGCCCTTTAATGGCTTTTACTCTT
CTTTGGATTGAAGGGAGCTCTACATCTTCTTTCACCTTCTCTATTTTTCTTT
CTTTATTTTCTCCTCTACAGTAATTTATTTGGATT AtmiPEP159a1 Arabidopsis
TTCCAAAACATGACGTGGCCTCTTCTCTCTCTCTCTTTCCTTCTCTCTAAG SEQ ID NO: 214
thaliana TATGTTTAGGGTTAATAATTAGGGTTCCTCCTCTCTTTTGTTCTGTCTTTAT
ATCTCCTTCATAGCTCTAATGTAAGAGATTTACCTCTTTTGGTGTTTTTGT
TAATCCACGTTCTCATCAAAACTTTCTCATTGTTTTATGAAGAGTTAAAG
GTCTTTACAGTTTGCTTATGTCAGATCCATAATATATTTGACAAGATACTT
TGTTTTTCGATAGATCTTGATCTGACGATGGAAGTAGAGCTCCTTAAAGT
TCAAACATGAGTTGAGCAGGGTAAAGAAAAGCTGCTAAGCTATGGATCC
CATAAGCCCTAATCCTTGTAAAGTAAAAAAGGATTTGGTTATATGGATTG
CATATCTCAGGAGCTTTAACTTGCCCTTTAATGGCTTTTACTCTTCTTTGG
ATTGAAGGGAGCTCTACATCTTCTTTCACCTTCTCTATTTTTTATTTTTCTT
TATTTCTACTCAACAATTATTTATTCGGATTCATCTTTAATTTTCCGTTAT
AATTTCTTTTTGGTAAGGATTATTCGCTATAATTTGAGAAT CrmiPEP159a Capsella
TTCCAACGAATGACGTGTACTCTCTCTGCTCTATCTCTCTCTCTAAATATG SEQ ID NO: 215
rubella TTTAGGGTTAATTAGGGTTCTTCATCTGTCTCTCTCTCTCTCTCTCTTCAG
AGTCTTTATAGCTTCTTCCAAGATTTTTAATTGAAAGTAATTTACCTCTTT
TGGAGTTCTGTACATATAGAATATCAGGAGTCGTGTTTCTTTTTTATCAA
GGTTCTCATCTAACCTTTATAGTATTTTCATTAGTTGATAAAGGTCTTCAT
AGTTTGCTTAGATCAGATCTTGTCTTCGTCTTTTCGATAGATCTTGTTCTG
TCCAATATACAGTGATTTTATTTCGAGAGCAAAAAAGATGAGAGGTAGA
GCTCCTTGAAGTTCAAACGAGAGTTTAGCAGGGTAGAGAAAAGCTGCTA
AGCTATGGATCCCATAAGCCCTAATCCTTGTTAATGATAAAGGATTTGGT
TATATGGCTTGCATATCTCAGGAGCTTTAACTTGCCCTTTAATTGCTTTTA
CTCTTCTTTGGATTGAAGGGAGCTCTACATCTTCTTTGACTTCTCTCTCTA
TTAAGTCTTTCTTTATTTTCTTCTCTACAATAGTTGTTTTGGATCGGAAGA
TCTTTAAGTTTCCCTTA AtmiPEP159b1 Arabidopsis
TTTCACTTTTGTTCTCCTCCTCCCTTTTTTTCTTTTCAGGATTCTTCTTTTCT SEQ ID NO:
216 AtmiPEP159b2 thaliana
ATGTTTTATCTTTCATAATAGATCTGATAATTTTGATTTTTCACTATATAT
ATTATGGTTAATACTAGTAGCTTTTTCATTTCAAGTTTTATCCTTCCATTG
GTTCTTTCTGAGTCAAATTGTCTCCTGTTTCGAACCATATATAAGTTTTCA
ATGGTTTTGTATTAACTCAAGTATTCAACATTATGTCTCTCTTTTTCTTGC
TTGGATCTCTAATGCTGTTCATATTTTAAAGCATAGGTTTAGGTTAGATG
CATGTAACTGCCAATTAAAAGAAGGTCAAGAGTTTTTTGATTGTATGAAT
ATATGAGTTAGTCAAAGCAGATCCACACGATTATATAGAAAAACAAAGG
AAGAAGAAGAGGAAGAGCTCCTTGAAGTTCAATGGAGGGTTTAGCAGGG
TGAAGTAAAGCTGCTAAGCTATGGATCCCATAAGCCTTATCAAATTCAAT
ATAATTGATGATAAGGTTTTTTTTATGGATGCCATATCTCAGGAGCTTTC
ACTTACCCCTTTAATGGCTTCACTCTTCTTTGGATTGAAGGGAGCTCTTCA TCTCTC
AtmiPEP160a1 Arabidopsis
CATCCCACCCTTAATTGTTTTATATAAACCATTTCTCCTCCTCTCTCCATC SEQ ID NO: 217
thaliana ACCTTCAATCTCTCTCGATCTCTCTCTGGATCCCCAATCTCACCTCCATGT
TTTGTTTGTTGATTCCCATCTTCTCTTTTGTCTTTTCACCAAATCGTCATTT
AAGGCTTCAAGAACAGTAACCCCAATTCCTCCACAAGAGGGAGAGAAAA
CAAAAGATCTTCCAATTCCATTCTCGTACATGCAAATCACAATCCATGCC
ATAGATTGTTTCTATTCCTCCTTATTTATTGCTTGTATCTGTTCATGCATG
GACCAGGTGGAGAGAGCATTACTTAAAAATAGAATTAGCTATCTGTTTTA
GGCGAATTAGTTTCCTTACATAACCATGTATATGTCATGACGCATATACA
TATGTAGATGTATATGTATTATATATGTATGCCTGGCTCCCTGTATGCCAT
ATGCTGAGCCCATCGAGTATCGATGACCTCCGTGGATGGCGTATGAGGA GCCATGCATAT
AtmiPEP160b1 Arabidopsis
ACTCATAACTCTCCCCAAATTCTTGACCAAAAATATCCGCCACTTTCTCTC SEQ ID NO: 218
AtmiPEP160b2 thaliana
TGGTTCATGTTTTCCCCTCAATGAAATACATACACATTTTGATTTTATTTA
AATCAAGATCGACGTATAAGCTATCCACCAATCATATTTAAGGGTTCCCG
TATACATATATACTATATATATATATGGAATAATAGTCGTGCCTGGCTCC
CTGTATGCCACAAGAAAACATCGATTTAGTTTCAAAATCGATCACTAGTG
GCGTACAGAGTAGTCAAGCATGAC AtmiPEP 161 Arabidopsis
CTCTAACTCATCCTTCTCTTCTATGAAAATTCCATTGTTTCTGCCGAAGCT SEQ ID NO: 219
thaliana TTGATCAGTACTTCTCTTTTGCTTGATCTCGGTTTTTGACCAGTTTATTGC
GTCGATCAATGCATTGAAAGTGACTACATCGGGGTTCCGATTTTTTTTGT
TCTTCATATGATGAAGCGGAAACAGTAATCAACCCTGGTTTAGTCACTTT
CACTGCATTAATCAATGCATTTGTAAAAAGAGGGAAAAGCA AtmiPEP162a1 Arabidopsis
CTAGAAGAAAAAACCAGATCTATAAAGTTTGTTATTAAAAGATAGAGAG SEQ ID NO: 220
thaliana AGAGGAGGGATGTAGTAGGCCAATAGGCAAATCAGAGAATCACAAATG
GTATCTGGTCAAGAAGATTCCTGGTTAAAACTTTCATCTCTCTGTTTCCTT
TTTCTTTCTTTGTTGGATTCATTAATTTGACATATCTCTATCATCACACTG
ATTCTCTTTCTCCCAGTTTGTCTGCAGATGCATGTGTGTAATCTAGGGTAT
ATGTTTTTGTCCATTTGGTTTCATAAGGCAATAAAGATCCAGCTATTTACT
ACTTGTGGTATAGATTTTGACTGTTGAATTTTCAGATCTGATGTGTTTCGT
TTGATCCGATTCGGAAAATTTATGTTTCGTTGACATTTTGGAGTTTAGTTG
GAAGAAGAGTGAGAGTCGCTGGAGGCAGCGGTTCATCGATCTCTTCCTG
TGAACACATTAAAAATGTAAAAGCATGAATAGATCGATAAACCTCTGCA
TCCAGCGTTTGCCTCTTGTATCTTTCTTATTGACTT AtmiPEP162b1 Arabidopsis
CTGCATCTATCCACCTCTCTCTGTAAATTTATCTAAATGTTTCTTTTAATC SEQ ID NO: 221
thaliana TTTTTGAGATTAATAATGATTTGTGTTTGTTCATCAACCGATTTTCTCAGA
TCTGTCAATTATTTTTGTTTATTTATTTATGATTTATGAATGAGGAAAGAG
TGAAGTCGCTGGAGGCAGCGGTTCATCGATCAATTCCTGTGAATATTTAT
TTTTGTTTACAAAAGCAAGAATCGATCGATAAACCTCTGCATCCAGCGCT GCTTGCTC
AtmiPEP163-1 Arabidopsis
TATCACAGTTCTCATCAAATATTTGAAAGTATCAAACAAAAAAAGGAGA SEQ ID NO: 222
AtmiPEP163-2 thaliana
GTGAGAAAAATAAAGAGAGAGATAGAGAGAGATCATGTCCACTACTCA
AGAGCATAGGTCTTGATTGGTGGAAGACAAGTACCTTAGATAAACCGAC
CAAAACCCGGTGGATAAAATCGAGTTCCAACCTCTTCAACGACAACGAT
TTCAACACTCTCTTCCAGGAACAACTTCCTCCAGGCAGATGATACTAAAG
TGCTGGAGTTCCCGGTTCCTGAGAGTGAGTCCATATCAAAATGCGCATTC
GTTATCACTTGGTTGAACCCATTTGGGGATTTAAATTTGGAGGTGAAATG
GAACGCGTAATTGATGACTCCTACGTGGAACCTCTTCTTAGGAAGAGCAC
GGTCGAAGAAGTAACTGCGCAGTGCTTAAATCGTAGATGCTAAAGTCGT
TGAAGAGGACTTGGAACTTCGATATTATCCCCCGTGT AlmiPEP164a1 Arabidopsis
AGTAGGGTTGGAAAATTTTTTTACATTTTTACTCTAAAATAGAATAGAGT SEQ ID NO: 223
AlmiPEP164a2 lyrata
TGGAGATGCCCTTAGCAGTTATTAGACAAGGGATTGTTTGGCCCTAGCGA AlmiPEP164a3
TCCTCTCTTCACTCTCTCACTTTTGTAGTTCAACCCTTCTTTTGCGTGAGAT
GCCATCATGGCATGACATGGTTCTTTTGCCTTACGTAAAACACACTCACG
CCAACACACGCCACATAACATAAATAAATTATATATACATATACGTATGT
GCGTGTGAGTCTTCCATTAATGCAATCTTTGGGCCTATATATATATACAA
ACCTTCCATAACCAAAGTTATCATACTACAAAAGCTCTCTCGTACTTGGA
AATGCGGGTGAGAATCTCCATGTTGGAGAAGCAGGGCACGTGCAAACCA
ACAAACACGAAATCCGTCTCATGTGTTTTGCACGTACTCCCCTTCTCCAA
CATGAGCTCCTGACCCATTG AtmiPEP164a1 Arabidopsis
AGACAAGCCCCCACACTAAAAAAACAGTAATATGGAATAAAAAAAAGC SEQ ID NO: 224
AtmiPEP164a2 thaliana
TTTCAAAACTTAGCAGTTATTAGACAAGGTATTGTTTGGCCCTAGCTAGC AtmiPEP164a3
GATCGTTTAGCTCTCTTCACTCTCTCACTTTTTTAGTTCAACCCTTCTTTTG
CGTGAGATGCCATCATGGCATGGTATGGTTCTTTTGCCTTACGTAAAACA
CACTCACGCCAGCACACACACACACACACATAACATATACGGATGTGCG
TGTGAGCTAGTCTTCCATTAATGCAATCTTTGGGCCTATATATACAAACC
TTTCCATAACCAAAGTTCTCATACTACAAACGCCCCTCATGTGCTTGGAA
ATGCGGGTGAGAATCTCCATGTTGGAGAAGCAGGGCACGTGCAAACCAA
CAAACACGAAATCCGTCTCATTTGCTTATTTGCACGTACTTAACTTCTCCA
ACATGAGCTCTTCACCC BrmiPEP164a1 Brassica rapa
AGACAACCCCACGTTTTAAAATAAGAAATGATGATAATTTTGTGGAAAT SEQ ID NO: 225
BrmiPEP164a2 AAAAGCTAGTATACTTTTGCAATAATTAGACAAGGTATTGATGCTTTGGG
BrmiPEP164a3 CCAAGCTAGTTTCTTTTAGCACTCTTCACTCACTAGTTTTTCTTCTCAGCC
CTTCTTTTGCGTGAAATGCCATCATGGCATGGCATTGTCATTTTGCCTTTC
GTAAAACACACTCACGCCAACATACATTATTCATATTCATGTGTATGTAT
ATGAATGTTCCATTAATGCAATCTTTGGGGCCTATATATACGAAGCTTAC
ATCACCAAAGCTCTCATATTACAAAAGCTCACATATATACTTGGAAATGT
AGGTGAGAACCTCCATGTTGGAGAAGCAGGGCACGTGCAAACCAAAAA
ACATGAAATCTGTTTCATATGCTTTGCACGTGCTCCCCTCCTCCAACATG A CpmiPEP164a1
Carica papaya AGACAACACTCCTCTTTGTTCCCTTCCTCACGTATCCACTTTTGAAATTTG
SEQ ID NO: 226 CpmiPEP164a2
TAATTTGTGTGCACCACCATGATTGCATGCCATCCCTACTTGCCTTTTCCC
CTTTTCCTTTCTCTAACATTTTACTCAATCTTCTTCTCCCCCTCCCCCCCTT
CCCCCTCTCTGCCATTATAACCATAATTAAACCTCTCTCCCTCTCTCTCCC
TCTCTCTCTCTCTCTCTCTGGGTTCTCAGTATAAATGCAGCTCTGCTTATA
CTTCCACACCTATATATATATACCTGACCCTTCTTCACCTCCTTCATCCAC
CTCCTCCTTCTTCCCCAAAACTTTCTTAACTGTTCTCTGCATACATATATA
TCCACATACATATATATATATATAGAGAGAGAGTGAGACAGAGAGGTTA
CCGAGGCAATTGGGTGAGTAGCTCCCTGTTGGAGAAGCAGGGCACGTGC
AAATTCTCCATGGCTTTCCCCTCTTTGCACGTGCTCCCCTTCTCCAACATG GGTTCC
CrmiPEP164a1 Capsella
CGGCCACCCCCACATTTAACAAGAAAAAAACTGATGGAATTAAAAGGTT SEQ ID NO: 227
CrmiPEP164a2 rubella
TGAGAACTTGGCAGTTATTAGACAAGGTATAGTTTGGCCCTAGCTTCTTT CrmiPEP164a3
TAATTTAGCTCTCTCCACTCTCACACTTTTCAACTTTCACCCTTCTCTTGC
GTGAGTCGCGAGATGCCATCATGGCATGGCATGGCATGTTTCTATTGCCT
TACGTAAAACACACTCACGCCAACACATACTCACTATACATGTAAATAA
GTATGTGCGCGTGTGAGTCTTCCATCCATCAATGCAATCTTTGGGGCTAT
ATATATACAAACCTTTTCCATAACCAAAGCTCTCATATAAACTACAAAAG
GCTCACTTGGGAAATGCGGGTGAGAATCTCCACGTTGGAGAAGCAGGGC
ACGTGCAAACCAACAAACACGAAACCCTCCTCATGTGCTTTGCACGTACT CCCCTTCTCCAACATG
GrmiPEP164a1 Gossypium
GAAAACCCAAGTTCAGGCTAACAAGTTATCTGATGATGAGATCAAGAAT SEQ ID NO: 228
GrmiPEP164a2 raimondii
TTTAAAGTTTCAATATAGATTTGGCATGGGTATTGGCGGCAGAAAGCAAT GrmiPEP164a3
TAAAAAACCAGTTATGTCAAATTCAAGGTCGTATCAGTTAAAATGAATG
AAGATTTAGAAATTTCAACAAGGAAGAGGACCCCACAGCTTTGTTAAAT
TAAGTGTGTGGTTTTTATAATTATCATCTCGAAAGTTTCATAATATCAATT
AGATTAAAACATCTCTGAATTTCATAATTACAAACCAGATAGATAGATAC
ATGAAAACTTAGACCCCAGAGATCTGTCTTTAAAGAATGCCCACTTCTAG
ACTCAATCTCTATTACTCTCTTTTTTTCTCTCTCTCTCTCTTCGGAAAAACT
TGTATATAAATAAATGACACTTTCTTTGCTTTCTGCACTCAACTCATGAAC
TTGAAAAGCTTTACTTGGATGGGTTGGTTGGGGGTGAGTATCTCTTGTTG
GAGAAGCAGGGCACGTGCAAGTTCCTATGTTTAAGTGAACTTTGCACGT
GCTCCCCTTCTCCACCGTGAG MtmiPEP164a1 Medicago
GAAGAGAAAAAACCTAGTGTAAAATTTGATATACTCTTTATGTATAGTAC SEQ ID NO: 229
MtmiPEP164a2 truncatula
GAATGTTTTTTTAAAAATTATGTAAAAAATGATAAAATAATAACTAACTA
AATTAACAGTAAAATTAGAAAAGTAAAATACTATGCCCAAATTTGATAT
TTTTTTTTATATATTTGTATAGATTATTATTATTTGATATGTAAAGTCCAA
TTAAAAATTTGTTTTAACTAAGATTTGAACTAGGTTTTCTTAAAAGACTC
ATCTTTTACTTCAAATTTATTTATCATTTGAATTCAATCACTTTCTAATATT
ATTATTATTATTTCCACCATACTCATTGCTTCTGCCACGTTACTTTAGTTA
GATCTCTTATGTCATATATCTCTCTCTCTCCTAAGTTGCTACCTATAAATA
CTAAGCCTTTCCCTTGGTTGGTTCAATTCAACTTCTACTTCTCATCAAACA
CAAAGTGCAATAAGCTTCATTTCCTGGGTGAGAAGCTCCTTGTTGGAGAA
GCAGGGCACGTGCAAATCCTCTTTCTGATTCATTCTCTCATAATGCATAT
CAATATCTTTTGCACGTGCTCCCCTTCTCCAACTAGG OsmiPEP164a1 Oryza sativa
ATGCAAACCCACTCCAACACTCCACAATCCACATACTCTCTCTCTCTCTCT SEQ ID NO: 230
OsmiPEP164a2 CTCTCTGAGTAGGAGTACATGTGTGTGTGTGATATCAATATGCATTCGAT
GTTGATGCTACTGTAGCCATCTTGTGGCTATATAAACCCAGCAGGCAGCA
GCACAGCTTAGCTAGAGAGCCATATTGCATGCACACTCGCTAATCTCTTT
TCTCTACTCTACTTGCATTACACCACCTCTGCATTGCACTTCAGTTCATTC
ATTCCACTGATGCATGGATCGATGTTGCTACCTTCTTCTCTTCTCCTCATG
CATCCATGCATCGATCTCACCTAGCTTCTTCCTCATCCTCTCTCGATCGAT
TACAAGAGAAAAGTGTTTGCTGTTCTTGCTATCGATCTACAGGTGAGTAG
GTTCTTGTTGGAGAAGCAGGGTACGTGCAAAATGCACACCGGTTGGTCG
AGCTAATTAACAAGCTCTGACGACCATGGTGATCGAATGCACGTGCTCCC
CTTCTCCACCATGGCCT AlmiPEP165a Arabidopsis
CTAGGGTTTAGGAATGACGACTTGTTTCTGTTGTGTCTTATTAAAAGCCC SEQ ID NO: 231
lyrata ATCTTCGTCTCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCA
TTCACCAACCTCTCTCTCTTTCTCTCTCTCCTCTCGATCTACAACAAAATG
TGAATCTGCTAAGATCGATTATCATGAGAATTAAGCTATTTCAGTTGAGG
GGAATGTTGTCTGGATCGAGGATATTATACATATATACATGTGTATGTTG
ATACATGTGATCATAGAGAGTATCCTCGGACCAGGCTTCATCCCCCCCAA
CATGTTATTGCCTCTGATCACCATATATATGTCGTTACATTTCATGGTTAA
TTACTTGCACAAATCACAAAAGCTTGGTTTGTAACTTTCTATGACCTTTTT
TAATGACTTTGAATCTTTCATGCATGACTTCTTAAGAGTAGATTTACACA
TTTGCGGATCCGTTTATGCTTTTTGCTTTTGTTTCGTTTATATATAT AtmiPEP165a
Arabidopsis CTAGGGTTTAGGAATGACGACCTGTTTCTGTTGTGTCTTATTAAAAGCCC SEQ
ID NO: 232 thaliana
ATCTTCGTCTCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCA
TTCACCAACCTCTCTCTCTCTCTCCTCTATCACTCTCTACAACAAAAATTT
GTGAATCTGCTAAGATCGATTATCATGAGGGTTAAGCTATTTCAGTTGAG
GGGAATGTTGTCTGGATCGAGGATATTATAGATATATACATGTGTATGTT
AATGATTCAAGTGATCATAGAGAGTATCCTCGGACCAGGCTTCATCCCCC
CCAACATGTTATTGCCTCTGATCACCATTTATTGTTACATTTTTTTTTGTTA
ATTACTTGCGCAAATTACAAAAGCTTGGTTTTTGTGATGACTTTGAATCTT
TCTTGCATGGCTTCTTAAGAGTAGATTTACGGATCCGTCTATGCTTTTTGC
TTTTTGTTTCGTTTATTTGTATTTAAAC BcmiPEP165a Brassica
GAGATCAATGAAATTATCCTGCCAAATAAAACGTGTGACGTTTATTCAAA SEQ ID NO: 233
carinata AATATATGCATTAGATGCTTTGATATTAAAATATTTCCTTTTAAAAGCTA
GCTAGGGTTTAGGAATGACGAGTTGTGTCTTATTAAAAGCCCTTCTTCTC
CTCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCATTCACCC
ACCTCTCCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGAACAACAAG
TGAGAATCTGCTAAAATATTGTGACTATTATCATGAGAATGAAGCTATTT
CAGTTGAGGGGAATGTTGTCTGGATCGAGGATATTATATATACACAAAT
ACGTATATATGTTAATACAAGTGTTTGATCATATATGTATATAGATTATT
CTCGGACCAGGCTTCATCCCCCCTAACATGTTATTGCCTCTGATCACCAG
ATTCTATCAACTCTTCGCTTATTATTTTGTCACAAACAAGTAATAAGCTCA
TAATTTTCTTTGAGTCTTTCAGCATCGTTTCATTATGTTTTTCGAATCCG BjmiPEP165a
Brassica GAGATCAATGAAATTATCCTGCCAAATAAAACGTGTGACGTTTATTCAAA SEQ ID
NO: 234 juncea AATATATGCATTAAATGCTTTGATATTAAAATATTTCCTTTTAAAAGCTA
GCTAGGGTTTAGGAATGACGAGTTGTGTCTTATTAAAAGCCCTTCTTCTC
CTCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCATTCATCCA
CCTCTTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
AGAACAACAAGTGAGAATCTGCTAAAATATTGTGATTATTATCATGAGA
ATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCGAGGATATTATA
TATACACAAATATGTATATATATGTTAATATCAGTGTTTGATCATATATA
TGTATATAGATTATTCTCGGACCAGGCTTCATCCCCCCTAACATGTTATTG
CCTCTGATCACCAGATTCTATCAACTCTTAGCTTATTATTTGTCACAAACA
AGTAATAAGCTCAATAATGTCTTTGAGTCTTTCAGCATCGTTTCATATGTT TTCGAATCCG
BnmiPEP165a Brassica napus
GATATCAATGAAATTATCCTGCCAAATAAAACGTGTGACGTTTATTCAAA SEQ ID NO: 235
AATATATGCTTTAAATGCTTTCATATTAAAATATTTCCTTTTAAAAGCTAG
CTAGGGTTTAGGAATGACGAGTTGTGTCTTATTAAAAGCCCTTCTTCTCC
TCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCATTCACCCA
CCTCTCCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGAACAACAAGT
GAGAATCTGCTAAAATATTGTGACTATTATCATGAGAATGAAGCTATTTC
AGTTGAGGGGAATGTTGTCTGGATCGAGGATATTATATATACACAAATA
CGTATATATGATAATACAAGTGTTTGATCATATATGTATATAGATTATTC
TCGGACCAGGCTTCATCCCCCCTAACATGTTATTGCCTCTGATCACCAGA
TTCTATCAACTCTTCGCTTATTATTTGTCACAAACAAGTAATAAGCTCAAT
AATGTCTTTGAGTCTTTCAGCATCGTTTCATATGTTTTCGAATCCG BomiPEP 165a
Brassica GGGATCAATGAAAATTATCCTGCCAAATAAAAACGTGTGACGTTTATCC SEQ ID
NO: 236 oleracea AAAAATATATGCATTAAATGCTGTGATATGAAGTATTTCCTTTAAAAGCT
AGCTAGGGTTTAGGAATTACGAGTTGTGTTTTATTAAAAGCCCTTCTTCT
CCTCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCATTCACCC
ACCTCTCCTCTTTCTCTCTCTCTCTCTCTCTCTCTAGAACAACAAGTGAGA
ATCTGCTAAAATATTGTGACTATTATCATGAGAATGAAGCTATTTCAGTT
GAGGGGAATGTTGTCTGGATCGAGGATATTATATATACACAAGTACGTA
TATATGTTAATACAAGTGTTTGATCATATATGTATATAGATTATTCTCGG
ACCAGGCTTCATCCCCCCTAACATGTTATTGCCTCTGATCACCAGATTCT
ATCAACTCTTCGCTTATTATTTGTCACAAACAAGTAGTAAGCTCAATAAT
GTCTTTGAGCCTTTCAGCATCGTTTCATATGTTTTCGAATCCG BrmiPEP 165a Brassica
rapa GAGATCAATGAAATTATCCTGCCAAATAAAACGTGTGACGTTTATTCAAA SEQ ID NO:
237 AATATATGCATTAAATGCTTTGATATTAAAATATTTCCTTTTAAAAGCTA
GCTAGGGTTTAGGAATGACGAGTTGTGTCTTATTAAAAGCCCTTCTTCTC
CTCCGCCACTCATCATTCCCTCATCATAACACCATCATCACCATTCATCCA
CCTCTTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
AGAACAACAAGTGAGAATCTGCTAAAATATTGTGATTATTATCATGAGA
ATGAAGCTATTTCAGTTGAGGGGAATGTTGTCTGGATCGAGGATATTATA
TATACACAAATATGTATATATATGTTAATATCAGTGTTTGATCATATATA
TGTATATAGATTATTCTCGGACCAGGCTTCATCCCCCCTAACATGTTATTG
CCTCTGATCACCAGATTCTATCAACTCTTAGCTTATTATTTGTCACAAACA
AGTAATAAGCTCAATAATGTCTTTGAGTCTTTCAGCATCGTTTCATATGTT TTCGAATCCG
AtmiPEP 166a Arabidopsis
CATCATCACCACTCACTTATCTTCTTCTCCATCTCTCTCTCTGCTTCTCCCT SEQ ID NO: 238
thaliana TAATCTTAGCCGGGTCTCGTGGGGGACGAACATAGAAAGAGAGAGATAT
AAAGATATATATTCAGAAACCCTAGATTCTATAATTTCGACTGAAAAGA
AAAAGGGGCTTTCTCTTTTGAGGGGACTGTTGTCTGGCTCGAGGACTCTG
GCTCGCTCTATTCATGTTGGATCTCTTTCGATCTAACAATCGAATTGAACC
TTCAGATTTCAGATTTGATTAGGGTTTTAGCGTCTTCGGACCAGGCTTCAT
TCCCCCCAATTGTTGCTCCCTGTTTACTCCATATTTCTTCCTTCTTTTCAAA
TTAGGGTTTCAGATCCAGTGAATGAACCCTTGTTAAAGGTTTGATCTCTT ACCTTACTTT
AtmiPEP166b Arabidopsis
TCTCATCATTCTCTTCATCATCACCACATTCATCTCTCTCTCTCTCTCTCTC SEQ ID NO: 239
thaliana TCTCTCTCTTTCTCTTCCTTGATCTTAGCCGGATCTGTTGGGGGACGAACA
CATGAGAGATAGATAAAATATAAGAAATTTCTCGAAAAAACCTAATAGA
AAAAGGTCTGTTTCTTAAAGAAGAAGAAGAAGAGGATTTAAAGAGGGAT
TTTTCTTTTGAGGGGACTGTTGTCTGGCTCGAGGACTCTTATTCTAATACA
ATCTCATTTGAATACATTCAGATCTGATGATTGATTAGGGTTTTAGTGTC
GTCGGACCAGGCTTCATTCCCCCCAA AtmiPEP167a Arabidopsis
TGATGAACAGAAAAATCTCTCTTTCTCTTTCTTGATCTGCTACGGTGAAG SEQ ID NO: 240
thaliana TCTATGGTGCACCGGCATCTGATGAAGCTGCCAGCATGATCTAATTAGCT
TTCTTTATCCTTTGTTGTGTTTCATGACGATGGTTAAGAGATCAGTCTCGA
TTAGATCATGTTCGCAGTTTCACCCGTTGACTGTCGCACCC AtmiPEP167b1 Arabidopsis
AACCACAAAGTACCGCTGCTATTTTCTTTTTACGTCTTTGTATTTGCATCG SEQ ID NO: 241
AtmiPEP167b2 thaliana
TCTAAGAGAATGATGGGTTGTTTTGTGGGATTTTAATGCAGGAGGAAAC
ATATGAGGGGTGATTAAGGCAAAAACCTTAAGATGTGGTCATTTAGATA
CATGGAGTCAAACTAAGAATGGACCTTGGCGAAAGCTTCTTCACGGTCA
AGATTTAAAATCAGGTACGACACTGTGTACGTGAGAGAGAGAGAGAGAG
AGAGAAAGAGATTATAGAAAGAAAGAGATGTATCACAATAAAGGAGTA
TATTTAGGGTCACAGGTGGTGGAGATATAGGTATGCAGGGCCAAGGCTC
TAATCTCTTCATAGCCCTATTGATTTTGTCCCTCTCTCTCTCTCTTTCTTCC
TCTCTTAGCTGTATGCATTATGATGCGTCTTTTAATTCACTGTTTCAGGCT
TCTTTAATTCGTGGTGTCTCTCTCCTTTTTACCCAACCATCTCTTAAAATTT
TTAACATCTGTTCCTCAAATCCTCTCTCATCTCTTTCTATAAGTATCTATA
GCGCCTCTTAAACCACAAAGCATCACCTCTGTCTTCTCTCATCTCCTTTCT
GTATTCTCTTTCATTGCCTTCACGTCTGTTGCAATTTCTCCACTTCTTGAG
CTTCCGTTTTTTACAATTATTGATCCGTCAAATATGTGAGATTTGCACAAC
TTGTTGCTCAGGTATTTTGAAGACAAGTCCACAAGGGAACAAGTGAAGC
TGCCAGCATGATCTATCTTTGGTTAAGAGATGAATGTGGAAACATATTGC
TTAAACCCAAGCTAGGTCATGCTCTGACAGCCTCACTCCTTCCT AtmiPEP169c1
Arabidopsis GAGCAAGACAATGCCACATACAAACTTGAAAGATCTCTTCATCTTTTCTC SEQ
ID NO: 242 AtmiPEP169c2 thaliana
CAAATGTTTTTTTTTCGTTTGCTATTTATCTCCACAATTCTTGGAACAAAA
ACTACATTCACAAACGAGAGAATTTTCACAACACCTCTTTTGCTCTCATT
TTTTTTTTTTCGTCCATTATGAGTATTAATTATGGTTAGGGAATCTTACAG
AATGAAAATGAAGGTGTGAATGGATTGTCTCATCTAAAGCCTTGAATGT
GGGAAAAAGGCCATTGTTGTTCAGCCAAGGATGACTTGCCGGTAGCTTG
TATTATGATTACTCTATATTCGATTTATATTATGGAGATGATGGTTTATAT
ATATTTACTTATCTACATAGTTTTAGTTGATTTTTTTTCGTACGTAATATA
ATACGAAAAAGTATTTACTTATTTATATATGTGTGTTGGGGCAAGAAGTG
TAACCAAGCTAGCCCGGCAAGTCATCTATGGCTATGCAACTGTCTCTTCC
TCTCATTCTAGGCTTACGATGACACGTAAAAAATCCCAAATATCACTAAT d Inventor
Nation AtmiPEP16911 Arabidopsis
AGGCATGAGACATAAAGAGAGTTAAATATAATGAAGAAGAGAGGTCTA SEQ ID NO: 243
thaliana ATATGGCGAAAAGAGTCATGTTTAATAGCCAAGGATGACTTGCCTGATCT
TTTTCACCTCCATGATTCAATTTTAAGTTCGTGGATTTTGGATTATTATGC
GTTTAAAAGGTATAATAATTTGAGATCATGTTGAATCTTGCGGGTTAGGT
TTCAGGCAGTCTCTTTGGCTATCTTGACATGCTTTCTTCATC AtmiPEP171a1 Arabidopsis
GAATTTTGATTTATGAACCTCCTCAAGAAGGAAAGACAGAGGAGGAGAC SEQ ID NO: 244
thaliana AAAGAAGTATAGGTTCACATTGCATAGCCAGTTTAGTTTTGAAGGATGG
ATATATGAAAAAAATATGAAGAGAGAGAAGAGAGAAGAAGAGGAGGAT
TAAAGAGGGTGAGGCCAGCTTTTGTGCTTTGGTAGTAGATGAGGTTTAAA
TGCTCCATACCTTCCATTTCCTTCTCTCTTACCCTAATTTAATTCTTCCTCT
CCTTTATAACTCCCCACAGACATTCTCACTTCTCCTCCTCACACTTCACAT
CAACACTTCTTTCTTGTTTTTTCATTTTACAATGTTTCCTTTGATATCCGCA
CTTTAAGCATGAGAGAGTCCCTTTGATATTGGCCTGGTTCACTCAGATCT
TACCTGACCACACACGTAGATATACATTATTCTCTCTAGATTATCTGATT
GAGCCGCGCCAATATCTCAGTACTCTCTCGTCTCTATTTTGGACTTTGTGG
TCTTGTAGATCGATTTGTATGTGTGTGTTGAAATGGAGACAAGTACTTGT
AACTTCTTTGTTGTTATATTGTTTACCTATAGGCTGATGTCATAAACTCTT
TTGATCTTGTTTCTAACTTCCAGATTCTTGAAAAATCAAGTCGTGTGTGTG
TCTCCATGGAAGCCTTTTCCATTTCTTCCTTTCCA AtmiPEP171b Arabidopsis
ACTCATAAACTTTGCTACTGGCCGCATTTCTATTTTCTCCTTCGATTCTTC SEQ ID NO: 245
thaliana TAATTCGTACTTTGGTTTCTGACGTCCCTAAAATTTTTAGACAGTAAGAG
TTTCTCCAGGATCCGATGGTTCTCTCCGGTAAATTAACATTTTAGTGTCAA
TAGTCATTTATACATATTTTTATTTCACTTTTTGTTTTGTTTATTGGTTTTC
TGGAGCTAAGTGGAGATTATAGTCGAACAAGAGTGGTTTTATGCAAGGT
AACGCGAGATATTAGTGCGGTTCAATCAAATAGTCGTCCTCTTAACTCAT
GGAGAACGGTGTTGTTCGATTGAGCCGTGCCAATATCACGCGGTAAACC
AAAAATGGCAAAGATAGTTATTATAACCTTAAAGGTATGTATCATTATCG
TTTTATTGTTTCAATTTTGATTAATGGCTTTGATATTTCATTTTTTTTTT MtmiPEP171b1
Medicago ATTGGTCAAACATACATACAGTAGCACTAGCTGGTTTCATTATTCCACTA SEQ ID
NO: 246 MtmiPEP171b2 truncatula
TGCTTCTTCATAGGCTCTCCAAATTTTGCAAAATTGAAAGAGACATAGTA
TATATATCTTAGCAAGGAGAAATTCAGGATATTGAGGATGAAGATTGAA
GAGTAATCAGTGATGAAGAAAGCAAGCAAGGTATTGGCGCGCCTCAATT
TGAATACATGGCTATAAAAATGCATCATATCAGCCATGTAGTTTGATTGA
GCCGCGTCAATATCTTGTTTCCATCTCCAAATTTACCAATCTCATCAAATC
AAATTAACACCACAATCAAGGCTTTCATTTAATGCAGTCAAAATAGGTTG
ACCTTATCATCGAAGAAATTGTTTTCTCATTCCTATCGAAGTTGGACTTGC
CGAAAATGCTCGAAAGCATGTGTTTTAGTTCGACAGGCGAAAAAGTTAC
CGAAGGACAATTTGGTTGTGGTTCGGATAAGATCAAGCAACGGATATTTT
CAAGACACGTTCGAAATTCAAATCAAATGGATAAGTATCGTTAGTTTACT
GCAGTTATAGTTTTAAATTCAAATCTAGGCAGTTGTTTCTATTTGTATAAA
TAGTAGTTTTTCCCTAGGGAAAAGGGGTCGCAATTCAATCATACAAAAA
ACTTACAATCAAATTATCCGCATGGAAGAGAGAAACGAGTCACAAGTTG
CAATGTATGAACATGTGTACCAATTTACATTCAATCAGTACAATTTAAGT
TCATTTCCATAAAAAAAAA ZmmiPEP171b Zea mays
ACCAGGGTTAGGTATCCATCCACACAGCAATGCATCTGCCTTCAACTCCC SEQ ID NO: 247
TCTCGCCCCCCACCCCAACACACATCTCTCTCTTTTCTAGGGAAGGAAAT
GACGAAGGGGACGACGACGGCATGCTTCGGCTAGCTCGTTGGTGCTAGG
ACAAGGGCGGAGGTATTGGCGCGCCTCAATCCGAAGGCGTGGCTGATAG
ATTGGCGCGGCAGCCATGTTCTTGGATTGAGCCGCGTCAATATCTCCCCT
TGCCTGTCCCGTACCTAGCTAGCTTGCTTGCCTCACTGATCGATGTCGTCC
CTATTTCATGGAGAAGCTGATGATTGATTATTCTCACAAGCAAGAACTGT
CTGATCTGTTGCCTGCATCGATCAGGATCTATATGCTGGAGAGTTCACAA
GAACATGGACAGAACTCGCTTCAACAACCGATCAATCGATTGATTAGGT
ATGTACCTACCTCATATGCCTCAGCTCTTCGTTATGGATTTCTTCAACCGA
AGGGTCAGTAAGCTCTTGGTTCCATGCCACTGCGTGAACTAAGCGTTCAC
AAAATCCGTTCCMCGGCATGAACCAAGCACTCAAAATCGCATGCAGCAT
CTTTCGTTTCAAAAAAAAATTGACTTYTGAAAACAATAGATGAATCAGTT
TCAAACATATATGATTATCCATTTTCTCAACCGGGAATTTATATCTCGTTG
GGATGCAAAACCGTTCAGTAGTAAAACTACTCCACGAGTATAAACTGTTT
CAGTTATTTTACTATTAATTAGTTACCCGTATGCTGTTATGGTTTCTATAT
ATCTATAAGTAAACCTTACTTAAATAAGATAGTTATACAAAAAAAAAAA AAAAA
AtmiPEP171c1 Arabidopsis
CAAGAAAAAACATTGAAATAGCTCATGTTGTCTCTTTCTCATTTTCATAT SEQ ID NO: 248
thaliana CTGCTAAAAAAAGAACCGTGTTTTCTAAACTGGTTTAACGGTAAGTACCT
GTCTCTAGTAACTTACCTATCAATTTGTTCCAATCATTTACTTGCTTTGAC
TTATTTGGTTTCCTTTTGTTTTGTTTTTCTTTAATATGTGGATGGAGTTTGG
TGTAATAAGCAACTGAAGAGTCGATGAGCGCACTATCGGACATCAAATA
CGAGATATTGGTGCGGTTCAATCAGAAAACCGTACTCTTTTGTTTTAAAG
ATCGGTTTATTTGATTGAGCCGTGCCAATATCACGCGTTTAAATAGTTTA
AAGATTCTATGTTAGTTGATGTGATCAATCAAGGTATGAATCTATATCAA
TTCTCTTATGCATAGTTTTATATTTACAGAGATGAGGTATTATCAATGTCT
ATCGTCGAGGATCACGCTCTTACTTATGTTATATTTCTATATAATTTTATT
AATTAGTTTTCTAAAAGAGAAGGACAATTTAAAATTATTTTAAAGAGTTT
TTTTTAAGTAGTTTTGTTTTCATGTTTATCTTCTGCAGGCTCTGAAGTTAG
GATAGTAACAAGAAAAAAGACAGAAAAAAAGAAGAAAATTCATATACA TTCGTGA
MtmiPEP171e Medicago
GAATAAGTGAATATTATCGATATTTATATCATATATCAACTTTTCTTCTGT SEQ ID NO: 249
truncatula GCTTGCTTGCAAATTTGCAATTAAGCTTTTTTGATCTTATGTAAGAGAAT
ATTATTGATGATGGTGTTTGGGAAGCCGAAAAAAGCGATGTTGGTGAGG
TTCAATCCGAAGACGGATTTACATGTATAGAGTTGTAAAATACGATCTCA
GATTGAGCCGCGCCAATATCACTTT MtmiPEP171h Medicago
CCACAAAACTATAACTAGCTAGAAGCTTTAATCGCCTTATTTATTATAAT SEQ ID NO: 250
truncatula AATAATAATAAATATGGCTTCAGCTGCAAAAGTATACATGGCGTGATATT
GATCCGGCTCATCTATATCTTCAAGTTCAATCATCCATATTCATATCAATT
TCAGACGAGCCGAATCAATATCACTCTTGTTTGCTTCATTGCATATTAATT
ATATACTTCATTTATAAGTTATAGTTTGCCATATATATATTAGATTGATTC
TGCAGAAGTAGACAGGAGTGGTGTTGTTTCTGCTCATCTTATTAAATAAT
GAATGAATGAATGACATTTGCTTACTTATAAGACGAGCCGAATCAATATC ACTCCAGTACACCT
AtmiPEP172a1 Arabidopsis
CTCTCTCTCTCTCTCTCTCTCATCTGTGTTCTAGATCTCACCAGGTCTTTCT SEQ ID NO: 251
AtmiPEP172a3 thaliana
CTGGTTAATATATGGCTTCCAAGATCTGGTAATATGTTATAAATACGTCA
TACTTAAGCTTTTTTCAAATCAAAAATAGAAATTTGTGGGTTTGTCTCGTT
TTACTATTTTAGCAGTATATATTAAGAAGTTCAGATGTTATTCGATCATCT
GTTTTTGCTTCCCCTCTGCCATCTTTATCTTTTAGGGTTTCAATTCTTTTTC
ACTTTTTTCTTCTGGTTTGGAGATGGTTAGGTTCCAACTAAGTATACGAG
ATTAAATTTGACATCTTAGTTACTTCAAAATTCCTTCAATCAAAACAAGT
CATCTCGACTATTCCGCCATGTTTGTATATACATATTTATATATTATATAT
ATGAAGGTACGAGTTTCTAGTGTCTATAAATTAAGAAGGTTAAGTACCAT
ATAGATGATATTTGTTAAGTAGTAAGTCACTCAAAGTTTGAGTTTGGGTT
TGAGTTTGAGTTTGAGTTTGAGTTTGAGAGACAAAAGATTACTACAAGA
AGATTGTTAAACAAAAATGGAAGACTAATTTCCGGAGCCACGGTCGTTG
TTGGCTGCTGTGGCATCATCAAGATTCACATCTGTTGATGGACGGTGGTG
ATTCACTCTCCACAAAGTTCTCTATGAAAATGAGAATCTTGATGATGCTG CATCGGC
AtmiPEP172b1 Arabidopsis
ACTTGCACCTCTCACTCCCTTTCTCTAACTAGTCTTGTGTGCACCCATTTA SEQ ID NO: 252
thaliana TGTGTACGTACTATTATCTCATAAATAAATATTTTTAAAATTAGATGCATT
TATTGATATGAAAAAGTTACAAGATTAGTTTGTTGTGTGTGAGACTTTGG
ATCGACAGATCGAAAAATTAACTAACCGGTCAGTATTGAATATCAACTA
TTATATGCTCCATGCATTCGCTTATAGTTTCACACAATTTGTTTTCTTCAC
GGTCTAAAATCAGAAGATTCCATATATTTTCTTATGACGTAAAAGGACCA
CTTATAAGTTGACACGTCAGCCCTTGGATTCGTGAGGTTTTTCTCTCTACT
TCACCTATCTACTTTTCCTCATATCCCACTGCTTTTCTCCTTCTTGTTCTTG
TTTTTCTCGTTTTTTTCTTCTTCTTCTCCAAGAAAATAGAGATCGAAAAGA
TTAGATCTATTTTGTGTAGCAAGAAATTATCATTTTCGTTTCTTCATTCAT
ATATTGTTCTATTATGTTGTACAATAATAGATACTCGATCTCTTGTGCGTG
CGTAAATTTTATACAAGTTGTCGGCGGATCCATGGAAGAAAGCTCATCTG
TCGTTGTTTGTAGGCGCAGCACCATTAAGATTCACATGGAAATTGATAAA
TACCCTAAATTAGGGTTTTGATATGTATATGAGAATCTTGATGATGCTGC ATCAAC
AtmiPEP172c1 Arabidopsis
TCACCAAATAGGCTCTTCTTTATCGCTTCATATATATAAAAGTCTACATCT SEQ ID NO: 253
thaliana ATCTCTTTCTAGGTCACTAGCTAGACTCTAGATTAAGGATTGAAATTAGG
GTTTCATGTTTCCAGCAAAATGGTGCCGTCTTGAGTCTTGAAAAGATCCA
AGACAAAACCAAATCACTACATACATCCCTATCATCAACCAGCTACTGTT
CGCTGTTGGAGCATCATCAAGATTCACAAATCATCAAGTATTCGTGTAAA
TAAACCCATTTATGATTAGATTTTTGATGTATGTATGAGAATCTTGATGA
TGCTGCAGCTGCAATCAGTGGCT AtmiPEP172e1 Arabidopsis
TGTCATATTGAGAACTCTTTAGCCTTTGGCTTCTGTTCCTGACACTTGTAT SEQ ID NO: 254
AtmiPEP172e2 thaliana
AGTGAAGTGGGCTTGTGTTATATAGATGGGATCTCTCTCTTTATTTAAAA AtmiPEP172e3
GTCAATTAGAGATCTTGATGCTACTTCTGTCCCTTTCCAAGTGATTTTACG
TCGACCAACTAGCTTTTTTCATATGAGTGTATATATTCATGTACCTATCTC
TCTCAATTGCTTCTCACCAAAATCATCTTGCTGATTCATTTGCTGTCTGAA
TCCTCTTGCTTTCCTCTTTGCTTTTTCATTTGTTGATTTAAACCATGGGAGT
TCCCAACTTTAGACCTCGAAACCGATAAGGATCTTTCTCTGCGGTTGAAA
TAGCTAGGTTCTCGATGAATAGGCTAGCCTTTGGTGGATGTTATCAGCCA
GTAGTCGCAGATGCAGCACCATTAAGATTCACAAGAGATGTGGTTCCCTT
TGCTTTCGCCTCTCGATCCGCAGAAAAGGGTTCCTTATCGAGTGGGAATC
TTGATGATGCTGCATCAGCAAATAC AcmiPEP319a1 Arabidopsis
TTGTATCCATAGTGTATTTCCTCGCATCTACCATCCATTTTCTACGCCTCT SEQ ID NO: 255
AcmiPEP319a2 cebennensis
CTCTCTCTCTTTCTCCATCAAATCTTGTTTTGTTCAAACTCTCTCTCTCATC
AATTCTCTCCATACAATACATGCATACATACATACATACCATCTCTAATA
TTTCATCAATCTTCTTTTGTTCCAAACGCTCTTTCTCTCCATTTACATACAT
ACGAATCATTGTTGTCATAGATCCGTTTAGAATTGCTTTAACTTTTAGATG
AGATCTAGGGTTTCTTTCTTTTTCTTCAAAATCATGCTTTTTCGCTTGCTA
GGTTATAGATCCATGTAAGTTTAGAGTAGATGTACACACACACGCTCGG
ACACTTATTAAATACATGTTGATACACTTAATACTCGCTGTTTTGAATTG
ATGTTGTAGGAATATATAAATGTAGAGAGAGCTTCCTTGAGTCCATTCAC
AGGTCGGATATGATCCAATTAGCTTCCGACTCATTCATCCAAATACCGAG
TCGCCAAAATTCAAATTAGACTCGTTAAATGAATGAATGATGCGGTAGA
CAAATTGGATCATTGATTCTCTTTGATTGGACTGAAGGGAGCTCCCTCTC TCTTCTGTATTCC
AhmiPEP319a Arabidopsis
TTGTATCCATAGTGTATTTCCTCGCATCTACCATCCATTTTCTACGCCTCA SEQ ID NO: 256
halleri CTCTCTCTTTCTCCATCAAATCTTGTTTTGATCAAACTCTCTCTCTCTCTCT
CTCATCAATTGTCTCCATACAATACATACATACCATCATCTTTCCCATCTC
TAATATTTCATCAATCTTCTTTTGTTCAAACGCTCTTCCTCTCCATATACA
TATACATACATACGAATCACATTGGTGTCATAGATCCGTTTAGAATTGCT
TTAACTTTTAGATGAGATCTAGGGTTTCTTTGTTTCTTTCGTTTTCTTCAA
ATTTTGCTGCATATTCTCCAAGATCATGATTTTTCGCTTGCTAGGTTATAG
ATCCATGCAAATATAGAGTAGATTTACACACACACACGCTCGGACACTT
ATTACATACATGTTGATACACTTAATACTCGCTGTTTTTAATTGATGTTGT
AGGAATATATATATGTAGAGAGAGCTTCCTTGAGTCCATTCACAGGTCGT
GATATGATCCAATTAGCTTCCGACTCATTCATCCAAATACCGAGTCGCCA
AAATTCGAACTAGACTCGTTAAATGAATGAATGATGCGGTAGACAAATT
GGATCATTGATTCTCTTTGATTGGACTGAAGGGAGCTCCCTCTCTCTTCTG TAT AlmiPEP319a
Arabidopsis TTGTATCCATAGTGTATTTCCTCGCATCTACCATCCATTTTCTACGCCTCT SEQ
ID NO: 257 lyrata
CTCTTTCTCCATCAAATCTTGTTTTGTTCCAACTCTCTCTCTCATCAATTCA
TTCCATACAATACATGCATACATACATACCATCATCATCTTTTCCCATCTC
TAATATTTCATCAGTCTTCTTTTGTTACAAACGCTCTTTCTCGCCATATAC
ATACATAAGAATCATTGTTGTCATAGATCCGTTTAGAATTGCTTTAACTTT
TAGATGAGATCTAGGGTTTCTTTCTTTTTCTTCAATTTTTGCTGCATATTCT
TCAAAATCATGATTTTTCGCTTGCTAGGTTATAGATCCATGCAAATATAG
AGTAGATGTACACACATTCACGCTCGGACACTTATTACATACATGTTGAT
ACACTTAATACTCGCTGTTTTGAATGGATGTTGTAGGAATATATATGTAG
AGAGAGCTTCCTTGAGTCCATTCACAGGTCGTGATATGATCCAATTAGCT
TCCGACTCATTCATCCAAATACCGAGTCGCCAAAATTCGAACTAGACTCG
TTAAATGAATGAATGATGCGGTAGACAAATTGGATCATTGATTCTCTTTG
ATTGGACTGAAGGGAGCTCCCTCT AtmiPEP319a1 Arabidopsis
TTGTATCCGCAGTGTATTTCCTCGCATCTACCATCCCTTTTCTACGCCTCT SEQ ID NO: 258
AtmiPEP319a2 thaliana
CTCCCTCTCTCTCTTTCTCCATCAAATCTTGTTTTGTTCAAACTCTCTCTCT
CTCATCTATTCTCTCCATACAATACATGAATATACATACATACCATCATCT
TCTTTTCCCATCTCTAGTTTTTCATCAATCTTCTGATGTTCCAAACGCTCT
ATCTCTTCATATACATACATACGAATATATTATTGTTGTCATAGATCCATT
TAGAATCACTTTAGCTTTTAGATGAGATCTAGGGTTTCTTTGTTTTCTTTC
AAATTTTGTTGCATATTCTTCTAAATCATGGTTTTTCGCTTGCTAGGTTAT
AGATCCATGCAAATATGGAGTAGATGTACAAACACACGCTCGGACGCAT
ATTACACATGTTCATACACTTAATACTCGCTGTTTTGAATTGATGTTTTAG
GAATATATATGTAGAGAGAGCTTCCTTGAGTCCATTCACAGGTCGTGATA
TGATTCAATTAGCTTCCGACTCATTCATCCAAATACCGAGTCGCCAAAAT
TCAAACTAGACTCGTTAAATGAATGAATGATGCGGTAGACAAATTGGAT
CATTGATTCTCTTTGATTGGACTGAAGGGAGCTCCCTCT BrmiPEP319a Brassica rapa
TTGTATCCATTGTGTATTTCCTTGCATCCATCAATAAATTTTATGTTACGC SEQ ID NO: 259
CTCTCTATTATTTCTCTCTACATCACACTGTCTTATGTTTAAGCTCTACTTC
TCAGCAATTCTCTCCACCCAATACATGCATACATACCATCATCGTATCGC
TCTAATTTTTCTATCAATCTTGTATCCTTCCACAAATTATCTTATGTCTCCC
ATTTTAAATCCTACATAGATCCACACATACGAATTATTCTTGTCTGAAGA
TCCATCCATTTACGATTGCTTTAACTTTTACATGAGATCTAGGGCTTCTTT
ATTTTTCTTCAAATCTTGCTGCATATATTTCAAGATCATGCTTTTCGGCTT
GCTAGGGTTCTAGATCCATGGATGTATAGCGTACATACATACACGCACTA
ATTCATACCTGTAGTTTGTACGGAGAACATCATAAAATATCACTGTTTGG
AATTAATCGTGTAGGAAATATAGATAGGTAGGGAGCTTCCTTTAGTCCAT
TCACAGATCATGATATGATCCAATTAGCTTCCGACTCATTCATCCAAATA
CCGAGTCACGAAAATTTCAACTTAGACTCGTTAAATGAATGAATGATGC
GGTAAACAAATTGGATCATTGATTCTCTCTGATTGGACTGAAGGGAGCTC CCTC CpmiPEP319a
Carica papaya CTATATCCCTGTCAAATAGTACTTGGTTTTGTTTTAGCCACAAATCTTCTG
SEQ ID NO: 260 GTCTTGCAAAGTTCCTTCTGATCTCTCCCCCTTTCTCATTTTTTCCTCCTCT
TATATATGGATCATCAATTTGCTGTACACACACACACATTTACTGTAGTG
ATAATTAGCTAGCTAATTTGTTAGTATGTAAATTAGATCCCAAGTACCCT
GTTATATTTTTTTAGGCTTATCCTATGCATACCTGATAGTACAAGAACTTA
GTTTGTAATTAGGTACTTGGTAGTAGGGTTAGATTAATTACTGTCTTGAA
AGAGAACTTATCCAACAAATAGAGCTATGAAGATTAAATTAGGTTTTAG
TCTTATTAAGATTATTATATTACTAGACAAAAACAGTTAAATTTTTTTAAT
TGGGTAATTAGGTACTTAGCAATAGGGTTAGATTAATTACTGTTTTGAAA
GAGAACCTACCTACAAATAGAGTTGAAATGATTATGTTTTAGTCTTACTA
AGATTGTCATATTTCTGGAAAAAAACAAATCTTGAAACAGATAATTCAG
ATAGTCATGATCAATGGAAAAAACATCATGGGTGTGTGCTTAATTAAGCT
AATATATATATATATGAAGATATAATGTTATGCACACTAGCTATGAATTT
GTAAGAATAATGAAGGATAAAGATGATATATTTAGATGTTATAAGTGTA
AGTAAGGTGGAATGGGTTGATGGGTAGTAGTAGTAGTAGTAGAGATGAT
TGGTGGAGAGAGCTTCCTTCAGCCCACTCATGGATGGGTATGAAGGGGT
AGAAGTAGCTGCCGACTCATTCATTCAGCCACTCAGTATGTAAACTCGTC
CCACTGTTGACTGTATGAATGATGCGGGAGATATTTTTACATCCATCTTT
CTCTGTGCTTGGACTGAAGGGAGCTCCTTCTT CrmiPEP319a Capsella
TTGTATCCATAGTGTATTTCCTCGCATCTACCATCTACTATTTTCTACGCC SEQ ID NO: 261
rubella TCTCTCTCTTTATCCCTCTATCTCTTTCTTCATCAAATCTTCTTTTGTTCAA
AGTCTCTCTCATCATTTTTCTCTATACACATACATGCATCCACATACATAC
ATACATATACCATCATCTTCTTTTCTCATCTCTAGTTTTTGTTTATAAATTT
TGTTCCAAGGATCTGTATCTCTCCAATAAAGATACATACAAATTATTGTT
GTCATAGATCTATTAGAATTGCTTTAACTTTTATATGAGATCTAGGATTTC
TTCCTTTTCTTTCAAAATTTGCTGCATATTTTTCAAAATCATGATTTATCG
CTTGCTAGGTTCTAGATCCATGCAAATTTAGAGTATTTTTACACACACAC
ACGCTTGGACACAAGTACATACATGTAGTTTTCTTTTATGTGGTGAAAAG
TACATAACATGTAGTTTATAGTTACTAGTCGCTATATAATTTAAAATTGA
TGTTATAGGAATATATGTACGGAGAGAGCTTCCTTGAGTCCATTCACAGG
TCGTGATATGATCCAATTAGCTTCCGACTCATTCATCCAAATACCGAGTC
TCACCAAAATTGGAACAAGACTTGTTAAATGAATGAATGATGCGGTAGA
CAAATTGGATCATTGATTCTCTTTGATTGGACTGAAGGGAGCTCC EgmiPEP319a
Eucalyptus TCGTTTCCCATCCCCATTTCATAGAATAATGCCACCAAACAAAGAAGCAT SEQ
ID NO: 262 grandis
TAGCTCAAAGACTAATTACCATCTGTTTTATTGATAGATACGTGCGAAAC
GGTGATTGTTTTTTCCCAAATAAGAAACCAAAATGAAGCATATTCAAAG
GTGGAGATATGGGGAGACTTCCGGAAGGCAAGGGGATTGGAAAAGGCT
CGAGATCAAAGTGCATAGCAACCCTTCGCTAAAGGTGAAAAAGAATACG
AATAACTTCAGTAGCTCACTTTAAATTCCGAAACATTAAACAAATCAAAT
CTCCCTCGCCCTCCTTGCCTCCTCTCTTTACCTATATAAAGCCACCGCCCC
TTCAATGAAATCCACGAGTGGAAGGTCACAGTATAGTAGGGTCCTGCAA
AGGGAGAGCGAGAGCGGCTCCACTGTCTACCTATAAGCAGTTCCTTTCTT
TTGTTTACATGTCTGTTGCACCTCACCGAGTTTTTCTATTCTCTTTCCTCTG
GTTGGTTAGCAGATTTCTCAGGGGACTTCCCTCCTCCCTTGAGGATCCTTC
TCTTGAAGCGATATGTCTCGAATGGGTAAGAGAGAGAGAGGAAGGGAGC
TTTCTTCAGTCCACCCATGGGACGTGTTGGGTTTTAATTAGCTGCCGACTC
ATTCATCCAAATACCGAGCGAGAGCAAGTAACAGAGCTCCGTAAATGAA
TGGATGATGCGGGAGTCTTGTTGATTCCCAAGCTTTCCGTGATTGGACTG
AAGGGAGCTCCCTCTATCT GrmiPEP319a Gossypium
GCGTATCCTTCCACTTACGGATTCACCATAGCTGCTATAGCCCGAGTTTG SEQ ID NO: 263
raimondii CTTGTCATAATAGAAATAAACTAAGGGAGAAAAAAGCTCTCCACTCTCG
CCTTTTTCTTCTTCGAGTCTCTCTCTTTGACTGGCCTTTGTGCAAAGATCTT
CCTTTTTTAAACAGTCGCTTTCTTTACTCTCCCTTTCCCTTCTTTCTCTTAA
TTGCTAAAGCAGCTTCCACTTCCACTCACTTTAACCCATGATCCATTTCAA
CCTGTCACAGTGGAGAGCAATTTGTATGGCTAATTTCCATCTCACCTATT
CTTTTCTGTTTGGGGTTCTCTAGTCTAGGGTTGCATGACATGAGAGACAT
GGCTCTTTTTTTTTTTTTTCAGTTCACAGGTGTTTATATATGTTGTTGTGTT
ATTTTGTCTTAAAGCTTTGTGATTGATGATCTGATAGGTAAGAGAGAGCT
TTCTTCAGTCCACTCATGGGATGGGGATGGGGTTTAATTAGCTGCCGACT
CATTCATCCAAATACTGTGTTACAAAACCCAGTAAATGAGTGAATGATGC
GGGAGACAAATTGAATCCTAATCTTCCTGTGCTTGGACTGAAGGGAGCTC CCTCCC
MtmiPEP319a Medicago
ATTTATCCAATCATGTTGCTCTCACTTCTTTTCAGCAGTTCTGTTGATATC SEQ ID NO: 264
truncatula ACCCTTTTAGTTGAAGCTTTGGATTCATGACTTTATGAGATGGTAAATCTT
TAAATTAAGTTTAAGTATGCACTTGATTTGTTTATATAATTTGTTTATTTA
GATTTTAAACCCTAAGTTGACTTTTTTAATTTGATTTAAATTATGATATGA
TTGTTATTTGGCTTACCATGGTCATAATTTAGGGTTTAGAAGATGCATGT
ATATCTTGAATTGTTTATGGTAATAAAGGGGTTAGGATTTCTCCTTTTGGT
GAAGTGAGAAAATCTCATAATTTTGTTCTGAAGGTAGTTTTTAAGATTTA
GGGTTATGGGTTCTTTGTTTGAATGCTTTTTCAAGTCTTTTTCAATGATAT
TTGCCTAGATCTGTTTTAATTTTGAATTAAAATTCTGGGTTTGGATTAATA
TTATTGAAGATTATTATTAATTAATTTATTAGAAATAGATGAAGAGAGCT
TCCTTCAGTCCACTCATGGAAGGGTAAGGGGTTTGAATTTACCTGCTGAC
TCATTGATTCAAACACAATAGACAATTATGGGGTTATGCTATTGTGAATT
GTGTGAATGATGCAGGAGGTGAATTTCTTCCTTTTCTTCTTTGCTTGGACT
GAAGGGAGTCTCCCTTT OsmiPEP319a Oryza sativa
TTATATCTGACGCGTTGTAATCCTGTTTAATTAGGGCTTTGCCCATTTCTT SEQ ID NO: 265
TTGACCCCTTCCGGACATTCGCTAGTTGGAACCTTGTTTTACTCCTAGCAG
TGTACTGTGTAGTACTTATTACGAGCAAACGTAAAAATAAATAAATGGA
AATGATACAAAGGCCGTGTTTAATTTTAAAATTTTTTTTCAAACTTTCAAC
ACTTTACATCCCATAAAAAGCTTACTATACATACAAACTTCCAACCTTTT
CGTCACATCGTATCTAATTTCAACTAAACTTTTAATTTTAGCGTGAACTA
AACACAGCCGAAGCCCGGCCACATTCTCACTATTTTTATTCATTTTATCAT
GCCTGTGATGTCACGCCTTGGCCCTATTTAATAGGCCTTCTCCATTTCTCT
CCATATGATGTCTTCTCTTCTCTATCCCTCTTGCCATCTTCTATCTTCCCTC
TTGCACCCATCTTTGTGATAACTTCTACTAGCTCCTCTCCTACTACCAGTC
ATACCACTCTCACAAATCCTCCAAGATCCGCATGGGGAGAAGCTCCAAA
AGTTTCGTGGTTAGTTTAATTTCATGCTTGTTTGCTGCCGTTTTTCATGTT
GATCTGATCTTAATATATGTAGACTGCTGTTAACATATTCTTTTAATTTGA
TGGAAGAAGCGATCGATGGATGGAAGAGAGCGATCCTTCAGTCCACTCA
TGGGCGGTGCTAGGGTCGAATTAGCTGCCGACTCATTCACCCACAATGCC
AAGCAAGAAACGCTTGAGATAGCGAAGCTTAGCAGATGAGTGAATGAA
GCGGGAGAGTAACGTTCCGATCTCGCGCCGTCTTTGCTTGGACTGAAGGG TGCTCCCTCCT
PpmiPEP319a Physcomitrella
TGGGATCCACACGAGTAATTATTCCCTCTCTCATGCATCACAACTTGGAC SEQ ID NO: 266
patens TTGCCCAGCTTTTACCTCTCTCCATGTTCCACCGTCGGAGATCCTCGGTGC
TGCTACCCCCGTTCGGCCAAACCCAACCCAACCCTAGGTGTCTGCCGGAC
CTCCGCTTCCCCTCCTGCTTCACCCCCTGCACCGCTTAAATTTGCACCTCG
TAGTATTTATCTATGCCCCATTTCAGATGACTTGCATGACACCCATCTGG
ATTCGTCTCCAGGCGCCTTGTCGTATCTATTCGCTATCAGTCTTTCTTTCA
GTCTGTCTTTCTGCCAAGCACACCGTGCTGGTGGTGGTGCTATCAGTCGA
AGCAGTCGCCGAAGGGTCCTTGTATCCAGCCCTCACCGGAGACAGTTCG
CTTCGGGGTGGGGAGAACTGTTGAGACCGTCGATGTTGCATGCAGCAGC
ACTGGCGAAGGTGCTTCTGATACTGGGTATTCCAGCCTCGCCGCTCGGTG
CACTGCAGGTACGTGGTTACATGACACTACTGTGTGGGTAGTCTGGGCAC
AAGTAGATCGACATGCCAGATTTGGCCCGTATGCGTGTATGTGCGGTTAT
GTTCCCGTTTCGATTGCGGATACCTTGATTGTGGAGCTCCGTTTCGGTCCA
ATAGTGGCTGCGACGGAAGGTGGTCCCGCTGCCGAATCACACGTCCGGG
TTGCTTATCGGGGCAGGGCCCCGATACGGTATCCGAACGTTTGTCCCGGG
AACTGGTCGACCTTCCGCCCGGCGTCTCTTGGACTGAAGGGAGCTCCACT ThmiPEP319a1
Thellungiella TTTTATACAAATAATGTTCGATAACACTAAACCCTAGCCATCCAACTAAT
SEQ ID NO: 267 ThmiPEP319a2 halophila
AGACAAAACCCTACTTGTAATTTACAACCGCAAATTCCCAGAGAACAGA
GTAACTACGAGAGAGAGATGGAGATTCAAATTAAAAAGAAAAACTTATA
TATAATGAATACACAAAAGCTACCTAATCTGTATATATATATATATAAAT
ATGTCTTCATTAAATTAATGGTCGTGGAATAGAAAAAGGAAAACCTAAT
TTGATCGCTAGGGCTTATCAGAGTAAAGATGGTTAACCTTCAAAAGATG
ACTAATTAACCGGGGAGATAATTAAAAGATTAAATACGCCAACAGAGAG
TTAAGAGATACCAGATTTAAATTCCACAATTTGGTCATGTTCTTCTTCAC
GTATTCATGACGATGTCTGAATTATAGAGAAACCCAAAATATAAAATGTT
AATTTTACCAGACATTTACATACCAATAACTCTATGACGATATGTAAAGT
AAGCAAGGCATGTTTTTATGCAGGGAAGATTGAAAATTCAAGATTAATC
AAGAAAATTGGAATACCAAAAAGAGAGGGAGCTCCCTTCAGTCCAATCA
GAGAGAATCAATGACCCAATTTGTCTACCGCATCATTCATTCATTTAACA
AGTCTAGCTCGAATTCTTGGTGACTCGGTATTTGGATGAATGAGTCGGAA
GCTAATTGGATCATATCACGACCTATGAATGGACTCAAGGAAGCTCTCTA
CAAATGTATTCCTACCACATCAACCCAAATATAGTGATTACAGATGCTGT
TCTCACTGTAGACTACATTTACGTTT AtmiPEP319b1 Arabidopsis
AGACATCTCTTCTTCTCTCATCTCTCTTTTCTTCTCTCTTTTCCTCACATAA SEQ ID NO: 268
thaliana ACTCTCTTTTTTTACTATTAAATCCATATGGTACCTCAAATTAATCTATGG
TCATCTAGGGTTATCTTGAAGATTAGAATTGATTCTAGCACGCACAGAGA
GGAAGATCATTGCATCCAGAATCACAAACATGGCCTATCTTTTATCTTTT
CTTTTTGATCTAAGTCACTGTTTTATGCTATATATAGTATAATCAAATTCT
TTACATGTGCTTGTATGTATGCGTATATATAGTAACGGAATTGTTAATAT
GCTTATAGATGTTGAGTTGGTGGAGGAAGAGAGCTTTCTTCGGTCCACTC
ATGGAGTAATATGTGAGATTTAATTGACTCTCGACTCATTCATCCAAATA
CCAAATGAAAGAATTTGTTCTCATATGGTAAATGAATGAATGATGCGAG
AGACAAATTGAGTCTTCACTTCTCTATGCTTGGACTGAAGGGAGCTCCCT AtmiPEP394a1
Arabidopsis TCTTATTCCATCACAATCATCTAGGGTTTTAAGCCAAGCTTATATAGCCC SEQ
ID NO: 269 thaliana
GTCATAAAGAGAACTCATCTGCCTCTCTCTCAATACCAATAAATATCACC
ACCGTCCTTCTCTCCTATCACTATTCAATCTATCGCAAACTCCTTTATGTC
TCTCCAATTTTATGAGAGGGTTTCCTTCAAGAACACAGTAAAATAGATTG
GATCTTTAAACTTTTGTTCCTTTTCATGAGGGTTTGACAAAGATTTTCTTA
CAGTCATCTTTGGCATTCTGTCCACCTCCTTCTATACATATATGCATGTGT
ATATATATATGCGTTTCGTGTGAAAGAAGGAGGTGGGTATACTGCCAAT AGAGATCTGTTAG
AtmiPEP395c1 Arabidopsis
TTGTATCATGACAGAGCAAGAAGAAGAAAGTCAAATGTCCACATGAGTT SEQ ID NO: 270
thaliana CCCTTTAACGCTTCATTGTTGAATACTCAAAGCCACATTGGTTTGTATATA
ACACTGAAGTGTTTGGGGGGACTCTTGGTGTCAT AtmiPEP395e1 Arabidopsis
TTTCAAACCCTAACACTCTTATAAACCGATTCGCCAAAATGTATCTACAA SEQ ID NO: 271
thaliana TATATTGATAATGTAATATCTATATATTCAAACAATCGTCGTGTTGGTCG
GATGTTTTCTAGAGTTCCTCTGAGCACTTCATTGGAGATACAATTTTTTAT
AAAATAGTTTTCTACTGAAGTGTTTGGGGGAACTCCCGGGCTGATTCGGT
ATTTTAAATTCAGTAGACTAGCTAGCTG AtmiPEP397b1 Arabidopsis
TGGTAATAGAAATGAGCAAGGAGATATTTTTTTCCCCTGGGTTTGAATGA SEQ ID NO: 272
thaliana ACATCATTGAGTGCATCGTTGATGTAATTTTACTTATTTTATTCCATTGTT
GAATTAATTAAAGAAGTATATATCAGCGTTGCATTCAATTATGTTTTTCT
AATTTTCAGGAAATACAAAAAAAATGAAAAAAAAAAATCACTTAAAAG
ACCTTGAGAGTTCTTTTGACT AtmiPEP398c1 Arabidopsis
GGATATCGAAACTCAAACTGTAACAGTCCTTTTATTACTGGTTTAGAAGA SEQ ID NO: 273
thaliana TAGATAAATATTGTTAAGGTAGTGGATCTCGACAGGGTTGATATGAGAA
CACACGAGCAATCAACGGCTATAACGACGCTACGTCATTGTTACAGCTCT
CGTTTCATGTGTTCTCAGGTCACCCCTGCTGAGCTCTTTCTCTACCGTCCA
TGTTTTATCAACGCCGTGGCCCGTG AtmiPEP399b Arabidopsis
TCTTATAGAGATGAAGAGAAACATGTAAACTCACTAGTTTTAGGGCGCCT SEQ ID NO: 274
thaliana CTCCATTGGCAGGTCCTTTACTTCCAAATATACACATACATATATGAATA
TCGAAAATTTCCGATGATCGATTTATAAATGACCTGCCAAAGGAGAGTTG CCCTGAAACTGGTTC
AtmiPEP399d1 Arabidopsis
CAATAACTCAAAATGCAATGTGAAATATGAAGAATATATTAAATAGTAG SEQ ID NO: 275
thaliana TGAAGATGCATGTTTATGAAGACAGAGAGATAATGTATGGTTGGATTAC
TGGGCGAATACTCCTATGGCAGATCGCATTGGCTAGATATGCAAGTAAA
ATGCTTCTCTGCCAAAGGAGATTTGCCCCGCAATTCATCC AtmiPEP403 Arabidopsis
ATTTAGGTCTCTCTTCTTCTTCTTCTTTTTCTTCTTGAGCGCCGGCGAAAA SEQ ID NO: 276
thaliana AAGTCTCTGTGAGAAAAAGATACGACGATTGTCATTAGAAGAGTCGTAT
TACATGTTTTGTGCTTGAATCTAATTCAACAGGCTTTATGTAAGAGATTCT
TTAACAATTCCTATAATCTTTGTTGTTGGATTAGATTCACGCACAAACTC
GTAATCTGTCTTTTCGATTTTTACCAGATCTGTC AtmiPEP447a1 Arabidopsis
AATTATATCCATGGTCATGGCTCATCATTAGTCGCACTGCTCTCCTTTTCT SEQ ID NO: 277
AtmiPEP447a2 thaliana
CAAAGTTTAAATTCGACATTTGGTAAAATGATGAAACCTCGATGGAACT
GCTCTCTTTATGGAATCACGGAATGGACAAATAATCAAAATCAGAAATC
GAAGCGAAAAGGGAGGAGAAAAACGCAGATTTGGAGGATTGGGGACAG
ATTAGATACTGTTGAATGCATCACTCTAATGCTATCAGCCTATTAATAGC
GTCCTATATTTTCGAAGACTTTTAATGTTTAGGGTTATGGATTTTTCGAGC
GAAGCATGGAGAGATGTTGAATTGGATACTATAGGATTTGGTACAACAC
ATACATATGTTCTGCTTCTGCAAAACTAACATATCAAGTTCAGAGAAACC
AGTAAGTCGTTGAATATTTTATTATCCATTCAACGCTTTCTTCTTTTGGAT
CATGTCTTGTTTGCTTGACCACTTCTTCTTGCTTAAGAGGATGGACAATAT
ATAAAAACTGGAGCCTTCTTTTTCTATGAATGCTTATCATCGCGGAGTTG
ATCTGTTCAATTCACCTGCCATTGGATGCTTTTTTTATATATACTTCACTG
TTCAATTTCAGATGCTTTAGAAGGTTTGCGGAGTAGCTAGAGAATCTGGT
ATCTTCAGTTCTTCAATTTCAGCTACTTGGTATCAGCTTCGTCATTGTATA
TCAACACATTCTTAATATATAATACTACTTTTTCATCCATTAAACCCCTTA
CAATGTCGAGTAAACGAAGCATCTGTCCCCTGGTATTGTCTTCGAGCTTG
GTGTTTTTTTCTAGCCAACTCCAAGTTCTCGAGTTGATCATTGTTTGTATT
CTTGAGACATTATTTGGGGACGAGATGTTTTGTTGACTCGATATAAGAAG
GGGCTTTATGGAAGAAATTGTAGTATTATATATCGAGAGTG AtmiPEP447b1 Arabidopsis
CTATAAATGCTGCTTATCATCGTGGAGTTGGTTCTGTAAACATTTGAAAA SEQ ID NO: 278
AtmiPEP447b2 thaliana
TTCTGAACAGTTTCACCTGCCATTGGATGCTTTGTTTCAATTTCAGGTGCG
TTAGAAGGTTTGCAGAGTAGCTAGAGAATCTCGTATCTTCACTTTCTGCT
ACTTGGTATCAGCTTCGTCACTTTATATCAACACATTCTTAATATACAATA
CTACTTTTTCATCCATTAATCCCCTTACAATGTCGAGTAAACGAAGCATC
TGTCCCCTGGTATTGTCTTCGAGCTTGGTGTGTTTTTCTAGCCAGCCCCAA
GTTCTCGAGTTGATCATTGTTTGTATTCTGACACATTATTTGGGGACGAG
ATGTTTTGTTGACTCGATATAAGAAGGGGCTTTATGGAAGAAATTGTAGT
ATTATATATTGAGAATG AtmiPEP447c Arabidopsis
TAGTATAACCGCTGATGTACACCTACCAGCTTGATAACTCTTTTTCGTGG SEQ ID NO: 279
thaliana TTTCTGTGTACTCGTTTCTGTTTGTACAGATACTTCTTGTTCAATTTCAGA
TGCTTTAGAAGGTTTTCGGAGTGGCTAGAGATCTGTTATCTGTATGAACA
GCTACTTGGTATCAGCTTCGTCATTTTATCAACACATTCTTAATATACAAT
ACTTCTTTTTCATGCATTAAGCCCCTTACAATGTCGAGTAAACAAAGCAT
GTGTCCGCTAATATTGTCTTCGAGCTTGGTATTTTTGTATTCTGATACGGT
ATTTGGGGACGACATCTTTTGTTGACTCGATATAAGAAGGGGGTTTGTGG
AAGAAATTGTAGTATTATATATCAAGAATG DmmiPEP1a Drosophila
TTTGTGGAACACATTCGACCCACTGAAAAATTGATATAATTTAATGAAAG SEQ ID NO: 280
DmmiPEP1b melanogaster
TGCATAAAAATGGTGGACAGTGCATTAAACTGAGCATTGAACACAAAGG
CCGCTCAGCAAATTGCTAATTAAAATTCACGATTGCCATTTCACCTGACA
CGTTGACGATTTTCATTACAATTCGATTATGTTTCGTTGCAGGGAATTTTA
AATGTTAATTGCCAAGAATGTTTCAACAAATTCATTTCTCATTAATGTGT
CTTTTCATTTAATTTTATGTTGTATGAGCTGCACGAGAAATGAGTTGTACT
TTTAGTTCGACGGCAGAGTCATGAATGTTCGGCAAAGAATGTAATAATA
ACTATCCTCTTTAGACAAATATAGATACAAATCTATCAGATTCTAAAAGT
AGAATAATCAATTAATCAGAAAGCTAAAAATAAATAGGCATATTTATAT
TTTAATGCGGATTTTTGAAGTTCAACGGGAGAAATGAATCCTTTTTACCA
GCCACAGGCGCAATTTGCAACAGAAAGTGTAGCAGAAGTACTCCTCGAA
TATTTCCCTGCTCCAGGAGTCATCCATGTGGTTTCGAGGCACACATTTGA
CAAACTCATGCCCCGCTATTTGTTGTAAAAACACAATCGCACACATGGCC
GCATTTCGGCGACTTCCAGAGAGCGGTACACTTAAGGCGGCCTGGGAAA
CGCCTGCAATCTGCTGGTCGCGAACTGCAGATTGCATCCATGTGCCAGGC
GACCATGCGACCATGTGACCATGTGCCCGCCCGACGCCTCGCAGCCCAC
ATCCTGCCCATCGAGGGCACAACTCAGCGTGGGTATTGCCGCTCCGGCTG
CTTCAAGTAGGTAAAAACCGAGAAGATTGAGGATGAATGTATGAGTATG
AGAAAATACTCGGCGGAACATATGCTGCCGGGCTTGACCTGACCCTGCC
TCATGTGTGGGTCTCCGATTTAATTTTAGGCACCTATATAAACGCGTGTTT
ACACTGCAGCCAGAACACAGTCGCCGTCTTCAGTTCGCGCCGTCAACTCC
TCGATCGATCGATCGCATCGTCTCGGATCGAATAGAGCTGGGCTTCTGCT
CCGGAGCTACATCGCCGTACTTGTCGGACGAGTGTGGTGATGAAAAGTC
GCTTAGTCCGGGATTCCTGCCAGATCTCTAAGGGATGAGCTGGCATCCCA
GGCTGGCCATGTGGCGCGAAGTATGCGCACAAAAAAGTCAAACAAAAA
GGCGCAATTTTATTACGGGCAACCAACGACGAAACAAAACAAAAGCCAA
CCGAAAAGCAGAAACAAAGCAGCAAAAAGTTTATGAATTTTTTGTGCAG
GCGCGTGAAAGATGCAAAACGAGAAAAAAACATGAAAAAAAAACATTA
AAAAAAACAAAAAAAATCCAAAACAGATACCGAGCTGTATCCGAAAAC
GAGTGGGGAAAGGGGTTTCCCAGTCACATATAAACACACTTCAGTGCGC
TTAAAAATTGCTTTATTGCAGTTGGACTATAAAAACGCACGGCAGCGAA
CACCGCACAACAAAAAGGACGAGCAGAAGTGGGCAAATAAAACGAAAG
CTCTTAAACGAAAAACAGGAAAATTTGCATGCCACAAAAATAAGCATAA
GGATTTGCCGCGCACAAAGTAGAAGCAAAAAGGAATTGCCCAAATGCAG
CCACAAAAGACTGTGGCAAATGTTTTGCAGCTTGCCCCTTTTTCCCTGCA
ATTACCGTCAGTCGTTGTCATTATTCAGCAGATTATATGGTTTTGCTTATT
CCGGACCACCATCATCATCATCATTATCATCATCTTCGGTAAGTTAGACA
ATCCCATAAAAAACTGTCCAAGTGAGTAGTGCCACCAAAAGTTAGCCGC
GTTGTGGAAAATCCAAAACAAAGACCATCCCATATTCAGCCTTTGAGAG
TTCCATGCTTCCTTGCATTCAATAGTTATATTCAAGCATATGGAATGTAA
AGAAGTATGGAGCGAAATCTGGCGAGACATCGGAGTTGAAACTAAAACT
GAAATTTGATTGAAACAGAAGTAGAACCGTAATGAAATGAATGAAATAT
TAACCCGTTTCTACAATCCCTGAATAAAATTATTAATTAATTATAGAGCG
GGCTAATTTTACAATATATATTGATTTTTTTTTGAAG DmmiPEP8 Drosophila
ATTCTTTTTTGGTGCTCGATCGTGACGGTTTGCTCGCGCTCTCCGCTGCGC SEQ ID NO: 281
melanogaster CGCTCTTTCCGTTGCATATGTGTGCGGGCGTTATTGTGCATGTTTCCGGTG
GCCGAAAAAAAATAGTAAAATAAAATATAGAAAACAGAAACCAAGAAT
AATAACAGCCATACGATAAACAGTGTGCCAATGTGTGTGTCTGTGTGTGT
GTGCATCTCGCGTAACAACAATAATTGCATTTATCGGATGGCGCCAGCTT
CAATTTAATTATAAATAACATGTTCAACTTTTTATACTATTTTCCCTGCGT
CAAAGTGGGCGTTGCAACTGCCCCCGGAAAATCACGCGCCCCGGTTCAA
AGTTAAAGTTTGCTGGGTAACGCACACACACACACACACAATCACTCAC
ACGCGGTCACACGCACATTTCAATAAACTAATG'GAGCCTGGCTTTGTTTT
TGTTTTATTTCCAACCCACTTGAGCACACAGCACACACAGAGAGAAAAA
TCAATACTCGTTATGGGATTAAATTTACAAAGCGCAAAGCAAAGCGACA
AACAAAATTCAAAAGAAAGAAAAAAAAACACTCAAATAAACTCACAAA
GAATTCCTTATCGCCAAGGGGGCCAATGTTCTAAGGTTCTTTCGCCTTGA
GAACTTTGAGCTTCCTCTGGCAAAGGAGATTATAATGTACAAATAATGTT
GCAATAACCAGTTGAAACCAATGGAATACCGAATCTTGCTAATTAGCAA
GGACATCTGTTCACATCTTACCGGGCAGCATTAGATCCTTTTTATAACTCT
AATACTGTCAGGTAAAGATGTCGTCCGTGTCCTTAACCTTCAGTACCACC
AACAGCAGCAGCAGCACCAAAAAAAAAAAAAAAAAAATGCGTAAAAAT
CCAAACAAATCATAAAAGTCGAAGGA HsmiPEP155 Homo sapiens
GCCGAGCCCGGGCCCAGCGCCGCCTGCAGCCTCGGGAAGGGAGCGGATA SEQ ID NO: 357
GCGGAGCCCCGAGCCGCCCGCAGAGCAAGCGCGGGGAACCAAGGAGAC
GCTCCTGGCACTGCAGATAACTTGTCTGCATTTCAAGAACAACCTACCAG
AGACCTTACCTGTCACCTTGGCTCTCCCACCCAATGGAGATGGCTCTAAT
GGTGGCACAAACCAGGAAGGGGAAATCTGTGGTTTAAATTCTTTATGCCT
CATCCTCTGAGTGCTGAAGGCTTGCTGTAGGCTGTATGCTGTTAATGCTA
ATCGTGATAGGGGTTTTTGCCTCCAACTGACTCCTACATATTAGCATTAA
CAGTGTATGATGCCTGTTACTAGCATTCACATGGAACAAATTGCTGCCGT
GGGAGGATGACAAAGAAGCATGAGTCACCCTGCTGGATAAACTTAGACT
TCAGGCTTTATCATTTTTCAATCTGTTAATCATAATCTGGTCACTGGGATG
TTCAACCTTAAACTAAGTTTTGAAAGTAAGGTTATTTAAAAGATTTATCA
GTAGTATCCTAAATGCAAACATTTTCATTTAAATGTCAAGCCCATGTTTG
TTTTTATCATTAACAGAAAATATATTCATGTCATTCTTAATTGCAGGTTTT
GGCTTGTTCATTATAATGTTCATAAACACCTTTGATTCAACTGTTAGAAA
TGTGGGCTAAACACAAATTTCTATAATATTTTTGTAGTTAAAAATTAGAA
GGACTACTAACCTCCAGTTATATCATGGATTGTCTGGCAACGTTTTTTAA
AAGATTTAGAAACTGGTACTTTCCCCCAGGTAACGATTTTCTGTTCAGGC
AACTTCAGTTTAAAATTAATACTTTTATTTGACTCTTAAAGGGAAACTGA
AAGGCTATGAAGCTGAATTTTTTTAATGAAATATTTTTAACAGTTAGCAG
GGTAAATAACATCTGACAGCTAATGAGATATTTTTTCCATACAAGATAAA
AAGATTTAATCAAAAAATTTCATATTTGAAATGAAGTCCCAAATCTAGGT
TCAAGTTCAATAGCTTAGCCACATAATACGGTTGTGCGAGCAGAGAATCT
ACCTTTCCACTTCTAAGCCTGTTTCTTCCTCCATATGGGGATAATACTTTA
CAAGGTTGTTGTGAGGCTTAGATGAGATAGAGAATTATTCCATAAGATA
ATCAAGTGCTACATTAATGTTATAGTTAGATTAATCCAAGAACTAGTCAC
CCTACTTTATTAGAGAAGAGAAAAGCTAATGATTTGATTTGCAGAATATT
TAAGGTTTGGATTTCTATGCAGTTTTTCTAAATAACCATCACTTACAAAT
ATGTAACCAAACGTAATTGTTAGTATATTTAATGTAAACTTGTTTTAACA
ACTCTTCTCAACATTTTGTCCAGGTTATTCACTGTAACCAAATAAATCTCA
TGAGTCTTTAGTTGATTTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAA AA AtmiPEP157c
Arabidopsis CTTTGTCACTTCATACACTCCCTATTGTCTATATATATATATATACTTACA SEQ
ID NO: 400 thaliana
CATATTCAAACATTATAATACTTAATTACACATACATACTTTATGATGTT
GCATATCACACATAGGTTTGAGAGTGATGTTGGTTGTTGACAGAAGATA
GAGAGCACTAAGGATGACATGCAAGTACATACATATATATCATCACACC
GCATGTGGATGATAAAATATGTATAACAAATTCAAAGAAAGAGAGGGAG
AGAAAGAGAGAGAACCTGCATCTCTACTCTTTTGTGCTCTCTATACTTCT
GTCACCACCTTTATCTCTTCTTCTCTCTAACCT AtmiPEP157d Arabidopsis
ATTTACTCTTCACCGCCCTCTCTCTATATATAGTCTCTATCCTCACATATT SEQ ID NO: 401
thaliana ATATATCAAACCGCAAGAATGCTGTATGTATAGTGGAGGGTGATAGTGT
GGTTGCTGACAGAAGATAGAGAGCACTAAGGATGCTATGCAAAACAGAC
ACAGATATGTGTTTCTAATTGTATTTCATACTTTAACCTCAAAGTTGATAT
AAAAAAAGAAAGAAAGATAGAAGAGCTAGAAGACTATCTGCATCTCTAT
TCCTATGTGCTCTCTATGCTTCTGTCATCACCTTTCTTTCTCTATTTCTCTC TAC
AtmiPEP160c Arabidopsis
AACCAAAACTCTTCAACATTTCTCTCTGACTACTTCATTTCCTCTTCCCAA SEQ ID NO: 402
thaliana CAGTTAAAAAAAGTTCTGATTCGATTCAAGCCAAGATCCACGTATAAAG
ATATGTTCATGCGTAGAGGTTTGGTATACAACAATATATACATATAATAG
TTTGTCGTTATGCCTGGCTCCCTGTATGCCACGAGTGGATACCGATTTTG
GTTTTAAAATCGGCTGCCGGTGGCGTACAAGGAGTCAAGCATGAC AtmiPEP164b
Arabidopsis ATACATTCTCTCTTTCTCTCTCTCTCTCTCTCTCATCCCGGCCCAGTTATGT
SEQ ID NO: 403 thaliana
GGTCGGAGAGAATGATGAAGGTGTGTGATGAGCAAGATGGAGAAGCAG
GGCACGTGCATTACTAGCTCATATATACACTCTCACCACAAATGCGTGTA
TATATGCGGAATTTTGTGATATAGATGTGTGTGTGTGTTGAGTGTGATGA
TATGGATGAGTTAGTTCTTCATGTGCCCATCTTCACCATC AtmiPEP166c Arabidopsis
TCACACATACCTTTCTTTCTCTTCTTCTTCTTACGAAAAGTTTCATCACAT SEQ ID NO: 404
thaliana TCACATTATCTTTAACTTTGGTCTCTTTTCTTTTTTGTCTCTTTTCTCTTCTT
GATAACGTGGTTCTAGTCTTGATTAATTCATTGTTGTGCGATTTAGTGTTG
AGAGGATTGTTGTCTGGCTCGAGGTCATGAAGAAGAGAATCACTCGAAT
TAATTTGGAAGAACAAATTAAGAAAACCCTAGATGATTCTCGGACCAGG
CTTCATTCCCCCTAACCTACTTATCGC AtmiPEP166d Arabidopsis
ATTTAGCTTCTTCTTCTTCTTCTTCTTCTGTCTACTTACATAAAGTTATCCTt SEQ ID NO:
405 thaliana GCTTTGGTTTAGGGTTGAGAGGAATATTGTCTGGCTCGAGGTCATGAAGA
AGATCGGTAGtATTGATTCATTTTAAAGAGTGAAATCCCTAAATGATTCTC
GGACCAGGCTTCATTCCCCCCAACC AtmiPEP169a Arabidopsis
TAGTATTCATAAGCACCAAAACAAATATGTAGAGATCTCCTCTTCCATTC SEQ ID NO: 406
thaliana TCTATTGTTACTTTCGAGAAGAAACATACAAAACACATACATTTTTCTTTT
GTTTGTGGTTTTCATATATACAAGTGGGTATAGCTAGTGAAACGCGAATG
TGACGAAAGTAGTGTGCAGCCAAGGATGACTTGCCGATTTAAATGATCTT
TCTTTATACTCTATTAAGACAATTTAGTTTCAAACTTTTTTTTTTTTTTTTT
TTTGAAGGATTCAGGAAGAAATTAGGATATATTATTCCGTATAAAATACA
AGATATATAAAACCAAAAAGAAAAAGTAACATGATCGGCAAGTTGTCCT
TGGCTACACGTTACTTTGTGTCGC AtmiPEP169h1 Arabidopsis
ACTCATCAACAACCTCTTCATAAATACATAAATCATATAAGAGAAAATG SEQ ID NO: 407
AtmiPEP169h2 thaliana GTGACATGAAGAATGAGAACTTGTGTGG AtmiPEP169n
Arabidopsis AGGCAAAAACATATAGAGAGTAATGAAGTGTATGATGAAGAAGAGAGG SEQ ID
NO: 408 thaliana TCTAACATGGCGGAAAGCGTCATGTTTAGTAGCCAAGGATGACTTGCCTG
ATCTTTTTCGCCTCCACGATTCAATTTCAAATTCATGCATTTTGGATTATT
ATACCTTTTAAAGTATAATAGGTCAAATATCATGTTGAATCTTGCGGGTT
AGGTTTCAGGCAGTCTCTTTGGCTATCTTGACATGCTTTTTCCATCCAT AtmiPEP170
Arabidopsis ATTCACTCCCTTCTTCTTCTTAATCTCCTTACAGTTACAGACATTCTCTCA SEQ
ID NO: 409 thaliana
CTTGCGTTCTTGTTTCTTTTACAAAACAGATACACTATGTTTCCGAGAGA
GTCCCTCTGATATTGGCCTGGTTCACTCAGATTCTCTTTTACTAACTCATC
TGATTGAGCCGTGTCAATATCTCAGTCCTCTCTCG AtmiPEP396a Arabidopsis
TCTCACAACTTCAACTTCCCTCTTTCTCTATATTACGCTTTTGCCCCTCACT SEQ ID NO: 410
thaliana CCCTCTTTCCACAATTAGGGTTTCGTCTGCTCTACATGACCCTCTCTGTAT
TCTTCCACAGCTTTCTTGAACTGCAAAACTTCTTCAGATTTTTTTTTTTTTC
TTTTGATATCTCTTACGCATAAAATAGTGATTTTCTTCATATCTCTGCTCG
ATTGATTTGCGGTTCAATAAAGCTGTGGGAAGATACAGAC AtmiPEP399c Arabidopsis
GAATAACCAACCAGCCTTCTCTCAAAGCAAACCAAAAAGAAAAACCAAC SEQ ID NO: 411
thaliana ATTGAAAGAGGAAGTTACGATAAGCGGAGCAGTAATAGGGCATCTTTCT
ATTGGCAGGCGACTTGGCTATTTGTATCTTTTGTGTTCTTGACTATTGGCT
ATGTCACTTGCCAAAGGAGAGTTGCCCTGTCACTGCTTCCGCTTAAACAC
AGTCTATAACCGGTTCTGCTAATATCAATCCTTCTTTTGGACATGTCCAA
AGCCGAGATTGATTGATAGAGAATTGGTCTCTCTGGCTACAAAACTAGTG
CGGTTCTCTCGATTTAAGTTTTAATAGCATTCACTTTGCACATTGCATCTT
TCACATCAAATTTCCATTTCATCAACCATCTAAACCTCTTTGTTAGCTTTG
ATATAAGCAACGATCTAAAGTCTAAAAACCATTAATCCTCTGAAAAAAA
AGACAATTTCGATGGTTCTATTATGTTTCTCCAATGCAGAAATTGTATCG
TCTGAATTATAGTAGATTTTTTCTAGACTAAAGTGTAAACCAAGACGAAT
CTGCACTAACAAGACACACCAATAGACTTTACAGAGAAAGGTTACGAGT
TTTGAAAATATTAACGGACCATAGTCATCGCG MtmiPEP169d Medicago
TGGATAAGTTAATTTATGGTCAAAGAGTCTTTCATGGAGAGGTTGAAAGT SEQ ID NO: 426
truncatula GAGATGAAGCCAAGGATGACTTGCCGGTATAATAGTAATTTGCCACAAA
TCTAGATAGCTATTAGCTATGTTTGGATGGGCGGTGAGATTAACAAAATT
ACAGCAGCATTGTGATTTTGTTGATGCTTTAAAGTGTAGTTTTTATCAAA
ATTACAGTGGTTCACTGTAATTATGAGAATCTCACCGTCAATCTAAATAT
GCATTTAGTTTCATTTCCGGCAGGTCATCCTTCGGCTATATT
TABLE-US-00004 TABLE 4 List of the microRNAs (miRNAs) miPEP
Organism Sequence of the miRNA SEQ ID AtmiPEP156a1 Arabidopsis
thaliana ugacagaagagagugagcac SEQ ID NO: 282 AtmiPEP156a2
AtmiPEP156a3 AtmiPEP156c1 Arabidopsis thaliana ugacagaagagagugagcac
SEQ ID NO: 283 AtmiPEP156c2 AtmiPEP156e1 Arabidopsis thaliana
ugacagaagagagugagcac SEQ ID NO: 284 AtmiPEP156f1 Arabidopsis
thaliana ugacagaagagagugagcac SEQ ID NO: 285 AtmiPEP159a
Arabidopsis lyrata uuuggauugaagggagcucua SEQ ID NO: 286
AtmiPEP159a1 Arabidopsis thaliana uuuggauugaagggagcucua SEQ ID NO:
287 CrmiPEP159a Capsella rubella uuuggauugaagggagcucua SEQ ID NO:
288 AtmiPEP159b1 Arabidopsis thaliana uuuggauugaagggagcucuu SEQ ID
NO: 289 AtmiPEP159b2 AtmiPEP160a1 Arabidopsis thaliana
ugccuggcucccuguaugcca SEQ ID NO: 290 AtmiPEP160b1 Arabidopsis
thaliana ugccuggcucccuguaugcca SEQ ID NO: 291 AtmiPEP160b2
AtmiPEP161 Arabidopsis thaliana ucaaugcauugaaagugacua SEQ ID NO:
292 AtmiPEP162a1 Arabidopsis thaliana ucgauaaaccucugcauccag SEQ ID
NO: 293 AtmiPEP162b1 Arabidopsis thaliana ucgauaaaccucugcauccag SEQ
ID NO: 294 AtmiPEP163-1 Arabidopsis thaliana
uugaagaggacuuggaacuucgau SEQ ID NO: 295 AtmiPEP163-2 AtmiPEP164a1
Arabidopsis lyrata uggagaagcagggcacgugca SEQ ID NO: 296
AtmiPEP164a2 AtmiPEP164a3 AtmiPEP164a1 Arabidopsis thaliana
uggagaagcagggcacgugca SEQ ID NO: 297 AtmiPEP164a2 AtmiPEP164a3
BrmiPEP164a1 Brassica rapa uggagaagcagggcacgugca SEQ ID NO: 298
BrmiPEP164a2 BrmiPEP164a3 CpmiPEP164a1 Carica papaya
uggagaagcagggcacgugca SEQ ID NO: 299 CpmiPEP164a2 CrniPEP164a1
Capsella rubella uggagaagcagggcacgugca SEQ ID NO: 300 CrmiPEP164a2
CrmiPEP164a3 GrmiPEP164a1 Gossypium raimondii uggagaagcagggcacgugca
SEQ ID NO: 301 GrmiPEP164a2 GrmiPEP164a3 MtmiPEP164a1 Medicago
truncatula uggagaagcagggcacgugca SEQ ID NO: 302 MtmiPEP164a2
OsmiPEP164a1 Oryza sativa uggagaagcaggguacgugca SEQ ID NO: 303
OsmiPEPl64a2 AlmiPEP165a Arabidopsis lyrata ucggaccaggcuucauccccc
SEQ ID NO: 304 AtmiPEP165a Arabidopsis thaliana
ucggaccaggcuucauccccc SEQ ID NO: 305 BcmiPEP165a Brassica carinata
ucggaccaggcuucauccccc SEQ ID NO: 306 BjmiPEP165a Brassica juncea
ucggaccaggcuucauccccc SEQ ID NO: 307 BnmiPEP165a Brassica napus
ucggaccaggcuucauccccc SEQ ID NO: 308 BomiPEP165a Brassica oleracea
ucggaccaggcuucauccccc SEQ ID NO: 309 BrmiPEP165a Brassica rapa
ucggaccaggcuucauccccc SEQ ID NO: 310 AtmiPEP166a Arabidopsis
thaliana ucggaccaggcuucauucccc SEQ ID NO: 311 AtmiPEP166b
Arabidopsis thaliana ucggaccaggcuucauucccc SEQ ID NO: 312
AtmiPEP167a Arabidopsis thaliana ugaagcugccagcaugaucua SEQ ID NO:
313 AtmiPEP167b1 Arabidopsis thaliana ugaagcugccagcaugaucua SEQ ID
NO: 314 AtmiPEP167b2 AtmiPEP169c1 Arabidopsis thaliana
cagccaaggaugacuugccgg SEQ ID NO: 315 AtmiPEP169c2 AtmiPEP16911
Arabidopsis thaliana uagccaaggaugacuugccug SEQ ID NO: 316
AtmiPEP171a1 Arahidopsis thaliana ugauugagccgcgccaauauc SEQ ID NO:
317 AtmiPEP171b Arabidopsis thaliana uugagccgugccaauaucacg SEQ ID
NO: 318 MtmiPEP171b1 Medicago truncatula ugauugagccgcgucaauauc SEQ
ID NO: 319 MtmiPEP171b2 ZmmiPEP171b Zea mays ggauugagccgcgucaauauc
SEQ ID NO: 320 AtmiPEP171c1 Arabidopsis thaliana
uugagccgugccaauaucacg SEQ ID NO: 321 MtmiPEP171c Medicago
truncatula agauugagccgcgccaauauc SEQ ID NO: 322 MtmiPEP171h
Medicago truncatula cgagccgaaucaauaucacuc SEQ ID NO: 323
AtmiPEP172a1 Arabidopsis thaliana agaaucuugaugaugcugcau SEQ ID NO:
324 AtmiPEP172a3 AtmiPKP172b1 Arabidopsis thaliana
gcagcaccauuaagauucac SEQ ID NO: 325 AtmiPEP172c1 Arabidopsis
thaliana agaaucuugaugaugcugcag SEQ ID NO: 326 AtmiPEP172e1
Arabidopsis thaliana ggaaucuugaugaugcugcau SEQ ID NO: 327
AtmiPEP172e2 AtmiPEP172e3 AcmiPEP319a1 Arabidopsis cebennensis
uuggacugaagggagcucccu SEQ ID NO: 328 AcmiPEP319a2 AhmiPEP319a
Arabidopsis halleri uuggacugaagggagcucccu SEQ ID NO: 329
AlmiPKP319a Arabidopsis lyrata uuggacugaagggagcucccu SEQ ID NO: 330
AtmiPEP319a1 Arabidopsis thaliana uuggacugaagggagcucccu SEQ ID NO:
331 AtmiPEP319a2 BrmiPEP319a Brassica rapa uuggacugaagggagcucccu
SEQ ID NO: 332 CpmiPEP319a Carica papaya uuggacugaagggagcuccuu SEQ
ID NO: 333 CrmiPEP319a Capsella rubella uuggacugaagggagcucc SEQ ID
NO: 334 EgmiPEP319a Eucalyptus grandis uuggacugaagggagcucccu SEQ ID
NO: 335 GrmiPEP319a Gossypium raimondii uuggacugaagggagcucccu SEQ
ID NO: 336 MtmiPEP3l9a Medicago truncatula uuggacugaagggagucucccu
SEQ ID NO: 337 OsmiPEP319a Oryza saliva uuggacugaagggugcucccu SEQ
ID NO: 338 PpmiPEP319a Physccomitrella patens cuuggacugaagggagcucc
SEQ ID NO: 339 ThmiPEP319a1 Thellungiella halophila
uggacucaaggaagcucucu SEQ ID NO: 340 ThmiPEP319a2 AtmiPEP319b1
Arabidopsis thaliana uuggacugaagggagcucccu SEQ ID NO: 341
AtmiPEP394a1 Arabidopsis thaliana uuggcauucuguccaccucc SEQ ID NO:
342 AtmiPEP395c1 Arabidopsis thaliana cugaaguguuuggggggacuc SEQ ID
NO: 343 AtmiPEP395c1 Arabidopsis thaliana cugaaguguuugggggaacuc SEQ
ID NO: 344 AtmiPEP397b1 Arabidopsis thaliana ucauugagugcaucguugaug
SEQ ID NO: 345 AtmiPEP398c1 Arabidopsis thaliana
uguguucucaggucaccccug SEQ ID NO: 346 AtmiPEP399b Arabidopsis
thaliana ugccaaaggagaguugcccug SEQ ID NO: 347 AtmiPEP399d1
Arabidopsis thaliana ugccaaaggagauuugccccg SEQ ID NO: 348
AtmiPEP403 Arabidopsis thaliana uuagauucacgcacaaacucg SEQ ID NO:
349 AtmiPEP447a1 Arabidopsis thaliana uuggggacgagauguuuuguug SEQ ID
NO: 350 AtmiPEP447a2 Arabidopsis thaliana uuggggacgagauguuuuguug
AtmiPEP447b1 Arabidopsis thaliana uuggggacgagauguuuuguug SEQ ID NO:
351 AtmiPEP447b2 Arabidopsis thaliana uuggggacgagauguuuuguug
AtmiPEP447c Arabidopsis thaliana ccccuuacaauguc gaguaaa SEQ ID NO:
352 DmmiPEP1a Drosophila melanogaster uggaauguaaagaaguauggag SEQ ID
NO: 353 DmmiPEP1b DmmiPEP8 Drosophila melanogaster
uaauacugucagguaaagauguc SEQ ID NO: 354 HsmiPEP155 Homo sapiens
uuaaugcuaaucgugauaggggu SEQ ID NO: 358 AtmiPEP157c Arabidopsis
thaliana uugacagaagauagagagcac SEQ ID NO: 412 AtmiPEP157d
Arabidopsis thaliana ugacagaagauagagagcac SEQ ID NO: 413
AtmiPEP160c Arabidopsis thaliana ugccuggcucccuguaugcca SEQ ID NO:
414 AtmiPEP164b Arabidopsis thaliana uggagaagcagggcacgugca SEQ ID
NO: 415 AtmiPEP166c Arabidopsis thaliana ucggaccaggcuucauucccc SEQ
ID NO: 416 AtmiPEP166d Arabidopsis thaliana ucggaccaggcuucauucccc
SEQ ID NO: 417 AtmiPEP169a Arabidopsis thaliana
cagccaaggaugacuugccga SEQ ID NO: 418 AtmiPEP169h Arabidopsis
thaliana uagccaaggaugacuugccug SEQ ID NO: 419 AtmiPEP169n
Arabidopsis thaliana uagccaaggaugacuugccug SEQ ID NO: 420
AtmiPEP170 Arabidopsis thaliana ugauugagccgugucaauauc SEQ ID NO:
421 AtmiPEP396a Arabidopsis thaliana uuccacagcuuucuugaacug SEQ ID
NO: 422 AtmiPEP399c Arabidopsis thaliana ugccaaaggagaguugcccug SEQ
ID NO: 423 AtmiPEP169d Medicago truncatula aagccaaggaugacuugccgg
SEQ ID NO: 427
TABLE-US-00005 TABLE 5 List of the control Pre-miRNAs Pre-miRNA
Organism Sequence of the Pre-miRNA SEQ ID Pre-miR169 Medicago
TTAGGGTTTTCAGCTCATGGTAATAAAAATGTCATCTAATGTCTTGCATGT SEQ ID NO: 359
truncatula GGGAATGAGGTCATATATGCAGCCAAGGATGACTTGCCGGCGAGCCTCTT
TCGATACTTTTATGACATAATTAATCATGTGGATAGCCAAGGTACTAAACT
CACTTTGCACTAAAACAAATATTTTTGCTTTAGTGCAAACTTAGTTTAGGC
GCTTCGCAACGGCTAGTCAAATGTCCTAGTTCCAATGTGATTGGTTGTCCG
GCAAGTCGTCTCTGGCTACGTAAAGGCCTCCTTTTTTCATGCTAGATTTTTG
ATGATTTGATATAGCCACACATATTTTGGAA Pre-miR169a Medicago
AAGAGGCAGAGAGAGTAATGCAGCCAAGGATGACTTGCCGACAACATTG SEQ ID NO: 360
truncatula GCGAATGTTCATGTGATTTCTGCCTCATTGTGCCGGCAAGTTGTCCTTGGCT
ATGTTAGTCTCTCATCTTCT Pre-miR171a Medicago
TGAATTCCCCTCCGCTTTTTGATGTTGGCTTGTCTCAATCAAATCAAAGTTC SEQ ID NO: 361
MI0001753 truncatula
TTGAAATTTGAGTTCTTTAGTCTGATTGAGTCGTGCCAATATCATATTAAG CGATAAAAGTC
Pre-miR171h Medicago
CCACAAAACTATAACTAGCTAGAAGCTTTAATCGCCTTATTTATTATAATA SEQ ID NO: 362
truncatula ATAATAATAAATATGGCTTCAGCTGCAAAAGTATACATGGCGTGATATTG
ATCCGGCTCATCTATATCTTCAAGTTCAATCATCCATATTCATATCAATTTC
AGACGAGCCGAATCAATATCACTCTTGTTTGCTTCATTGCATATTAATTAT
ATACTTCATTTATAAGTTATAGTTTGCCATATATATATTAGATTGATTCTGC
AGAAGTAGACAGGAGTGGTGTTGTTTCTGCTCATCTTATTAAATAATGAAT
GAATGAATGACATTTGCTTACTTATAAGACGAGCCGAATCAATATCACTCC AGTACACCT
Pre-miR393a Medicago
AACTGCAACTTGAGGAGGCATCCAAAGGGATCGCATTGATCCTATAATAT SEQ ID NO: 363
truncatula TTCAACTTTAGTCACTTTAATTTTCTCTCATATAATACTTAATTGGGATCAT
GCCATCCCTTTGGATTTCTCCTTTAGTAGCTAC Pre-miR393b Medicago
AGGCATCCAAAGGGATCGCATTGATCCCAAATCTAATTAAGTCCCTAGCTA SEQ ID NO: 364
truncatula CTTAATTAACAACTTAATTTCCTTAATATCTCATAATATTTGGGATCATGCT
ATCCCTTTGGATTCAT Pre-miR396a Medicago
TGCTTTTCCACAGCTTTCTTGAACTTCTTTCGTATCTTAAATCTGTTTTCAA SEQ ID NO: 365
truncatula GATTAAAAGTCCTAGAAGCTCAAGAAAGCTGTGGGAGAATA Pre-miR396b
Medicago TATTCTTCCACAGCTTTCTTGAACTGCATCCAAATTGAGTTCCTTTGCATTG SEQ
ID NO: 366 truncatula
CCATGGCCATTGTTTTGCGGTTCAATAAAGCTGTGGGAAGATA
TABLE-US-00006 TABLE 6 List of the control miRNAs miRNA Organism
Sequence of the miRNA SEQ ID miR169 Medicago truncatula
CAGCCAAGGAUGACUUGCCGG SEQ ID NO: 367 miR169a Medicago truncatula
CAGCCAAGGAUGACUUGCCGA SEQ ID NO: 368 miR171a Medicago truncatula
UGAUUGAGUCGUGCCAAUAUC SEQ ID NO: 369 miR171h Medicago truncatula
GAGCCGAAUCAAUAUCACUC SEQ ID NO: 370 miR393a Medicago truncatula
UCCAAAGGGAUCGCAUUGAUC SEQ ID NO: 371 miR393b Medicago truncatula
UCCAAAGGGAUCGCAUUGAUC SEQ ID NO: 372 miR396a Medicago truncatula
UUCCACAGCUUUCUUGAACUU SEQ ID NO: 373 miR396b Medicago truncatula
UUCCACAGCUUUCUUGAACUG SEQ ID NO: 374
TABLE-US-00007 TABLE 7 Polymorphism of the DNA sequence of the
different regions of pri-miR171b # # % # Size SNPs mutations SNP
haplotypes pri-mir171b 1127 91 100 8.07 161 5' pri-miR171b 129 4 4
3.1 5 miPEP171b 62 2 2 3.22 3 Pre-miR171b 118 1 1 0.85 2 miR171b +
miR171b* 42 0 0 0 1 3' pri-miR171b 259 39 42 15.06 89
[0771] The following examples will illustrate the invention better,
but without limiting its scope.
EXAMPLES
[0772] A: Analysis of the miPEPS in Plants
Example 1
Characterization in the Model Plant Medicago truncatula
[0773] a) Identification and characterization of MtmiPEP171b1
(miPEP171b1 identified in Medicago truncatula)
[0774] The microRNA MtmiR171b is expressed in the meristematic
region of the roots. The overexpression of this microRNA in
particular leads to a reduction in the expression of the genes HAM1
(Accession No. MtGI9-TC114268) and HAM2 (Accession No.
MtGI9-TC120850) (FIG. 1A), as well as to a reduction in the number
of lateral roots (FIG. 1B). The overexpression of the pri-miR171b
also induces a decrease in the number of lateral roots (FIG.
37).
[0775] The sequence of the primary transcript of MtmiR171b was
determined using the RACE-PCR technique. Analysis of the sequence
of the primary transcript made it possible to identify the presence
of several completely unexpected small open reading frames (sORFs).
These sORFs were called miORFs for "microRNA ORFS". These miORFs
potentially encode short peptides, from about 4 to 100 amino acids.
No significant homology relating to these miORFS was found in the
databases.
[0776] The overexpression of the first miORF, called MtmiORF171b,
leads to an increase in the accumulation of MtmiR171b and a
reduction in the expression of the HAM1 and HAM2 genes (see FIG.
2A), as well as to a reduction in the number of lateral roots (FIG.
2B), as was already observed in the overexpression of
MtmiR171b.
[0777] In order to determine whether MtmiORF171b leads to the real
production of a peptide and whether the regulatory function
observed above is indeed carried by said peptide, a synthetic
peptide, the sequence of which is identical to that potentially
encoded by MtmiORF171b, was applied on the roots of Medicago
truncatula. Application of this peptide leads to the phenotype
already observed above in the overexpression of MtmiORF171b, i.e.
it leads to an increase in the accumulation of MtmiR171b and a
reduction in the expression of the HAM1 and HAM2 genes (see FIG.
3A), as well as a reduction in the number of lateral roots (FIG.
3B).
[0778] The results of these experiments demonstrate that
MtmiORF171b encodes a peptide capable of modulating the
accumulation of MtmiR171b, and the expression of the target genes
of MtmiR171b: HAM1 and HAM2. Said peptide has been called
MtmiPEP171b1 ("miPEP" corresponding to microRNA encoded
PEPtide).
[0779] Moreover, MtPEP171b1 leads to an increase in the
accumulation of MtmiR171b (FIG. 4A) and of pre-MtmiR171b (FIG.
4B).
[0780] b) Specificity of miPEP171b1
[0781] The expression of different microRNA precursors of Medicago
truncatula (MtmiR171b SEQ ID NO: 319, MtmiR169 SEQ ID NO: 367,
MtmiR169a SEQ ID NO: 368, MtmiR171a SEQ ID NO: 369, MtmiR171h SEQ
ID NO: 370, MtmiR393a SEQ ID NO: 371, MtmiR393b SEQ ID NO: 372,
MtmiR396a SEQ ID NO: 373 and MtmiR396b SEQ ID NO: 374) was
determined and compared between control plants and plants in which
MtmiORF171b encoding MtmiPEP171b1 was overexpressed (FIG. 5A), or
between control plants and plants grown on culture medium
containing MtmiPEP171b1 (FIG. 5B).
[0782] The results obtained indicate that MtmiPEP171b1 only leads
to an increase in the accumulation of MtmiR171b and not of the
other miRNAs, which indicates that a miPEP only has an effect on
the microRNA from which it is derived.
[0783] c) Localization of miPEP171b1
[0784] Moreover, the immunolocalization of miPEP171b1 in the roots
of M. truncatula reveals the presence of miPEP171b1 in the lateral
root initiation sites, showing a co-localization between the
microRNA and the corresponding miPEP (FIGS. 28 and 34).
Example 1B
MtmiPEP169d
[0785] Regarding MtmiPEP169d, it has been demonstrated in vivo in
M. truncatula that the overexpression of this miPEP induces an
accumulation of MtmiR169d (FIG. 32).
Example 1C
MtmiPEP171e
[0786] Regarding MtmiPEP171e, it has been demonstrated in vivo in
M. truncatula that the overexpression of this miPEP induces an
accumulation of MtmiR171e (FIG. 33).
Example 2
Characterization in the Model Plant of Tobacco
[0787] a) Conservation of the Mechanism in Tobacco
[0788] In order to determine whether the mechanism of regulation of
the microRNAs is conserved in other plant species, the regulation
of MtmiR171b by MtmiPEP171b1 was tested in a different cellular
model. For this, MtmiR171b and MtmiPEP171b1 were introduced into
tobacco leaves.
[0789] The accumulation of MtmiR171b was measured in tobacco leaves
transformed in order to express MtmiR171b of Medicago truncatula
starting from wild-type pri-miRNA capable of producing
MtmiPEP171b1, or starting from a mutated version of pri-miRNA
incapable of producing MtmiPEP171b1 (in which the start codon ATG
of MtmiORF171b has been replaced with ATT) (FIG. 6 and FIG. 20).
Absence of translation of MtmiPEP171b1 leads to a marked decrease
in the accumulation of MtmiR171b.
[0790] The accumulation of pre-MtmiR171b was measured in tobacco
leaves transformed in order to express MtmiR171b of Medicago
truncatula alone (control), or additionally expressing the
wild-type MtmiORF171b of Medicago truncatula (35SmiPEP171b1 ATG),
or a mutated version of MtmiORF171b in which the start codon ATG
has been replaced with ATT (35SmiPEP171b1 ATT) (FIG. 7 and FIG.
21). The expression of MtmiORF171b leads to an increase in the
accumulation of pre-miR171b, and this accumulation of pre-miR171b
depends on the translation of MtmiORF171b to micropeptide.
[0791] Moreover, in the tobacco leaves transformed in order to
express MtmiR171b of Medicago truncatula, untreated or treated by
spraying with MtmiPEP171b1 (0.1 .mu.M) for the first time 12 h
before sampling and then a second time 30 minutes before sampling,
it was observed that MtmiPEP171b1 may be used directly in peptide
form by foliar sprayings (FIG. 8).
[0792] Moreover, it was observed in tobacco (as in Medicago
truncatula) that the MtmiPEP171b1 leads to an increase in the
accumulation of MtmiR171b (FIG. 9A) and of pre-MtmiR171b (FIG. 9B),
but reduces the accumulation of pri-MtmiR171b (FIG. 9C).
[0793] Taken together, these results indicate that the mechanism of
regulation of the microRNAs and of their target genes under the
control of miPEPs is conserved between the species.
[0794] b) Intracellular Localization of MtmiPEP171b1
[0795] Tobacco leaves were transformed in order to overexpress
MtmiPEP171b1 of Medicago truncatula fused with a fluorescent
protein (GFP) (FIG. 10). The results obtained indicate that the
miPEP is localized in small nuclear bodies.
[0796] c) Identification of miPEPS from Databases
[0797] Genomic databases of plants were searched for the presence
of open reading frames within primary transcripts of 70 miRNAs, and
101 miORFs capable of encoding a miPEP were identified.
[0798] At present, AtmiPEP165a and AtmiPEP319a2, identified in
Arabidopsis thaliana, have already been characterized. The
experiments conducted in the model plant of tobacco made it
possible to demonstrate that the overexpression of AtmiORF165a or
of AtmiORF319a leads to an increase in the accumulation of
AtmiR165a or of AtmiR319a respectively (FIG. 11).
[0799] miR165a regulates transcription factors such as Revoluta,
Phavoluta and Phabulosa.
[0800] miR319 regulates genes of the TCP family.
Example 3
Characterization in the Arabidopsis thaliana Model Plant
Example 3A
AtmiPEP165a
[0801] Regarding AtmiPEP165a, it has been demonstrated in vivo in
Arabidopsis thaliana that treatment with AtmiPEP165a leads to a
phenotype with greatly accelerated root growth (FIG. 12).
[0802] Moreover, treatment of plants with higher and higher
concentrations of miPEP165a shows a dose-dependent effect on the
accumulation of miR165a and the negative regulation of its target
genes (PHAVOLUTA: PHV, PHABOLUSA: PHB and REVOLUTA: REV) as a
function of the amount of miPEP165A (see FIG. 22).
Example 3B
AtmiPEP164a
[0803] Regarding AtmiPEP164a, this was synthesized and was used for
investigating an increase in the accumulation of miR164a in roots
of A. thaliana treated with the synthetic peptide.
[0804] Northern blot analyses indicate that treatment of the plant
with the peptide miPEP164a leads to an increase in the accumulation
of miR164a (FIG. 23).
[0805] The same type of results has been obtained by qRT-PCR (FIG.
30).
[0806] It has also been demonstrated in vivo in Arabidopsis
thaliana that treatment of the plant with AtmiPEP164a increases
plant growth significantly (FIG. 24).
Example 3C
AtmiPEP165a
[0807] Regarding AtmiPEP165a, this was synthesized and was used for
investigating an increase in the accumulation of miR165a in roots
of A. thaliana treated with the synthetic peptide.
[0808] Northern blot analyses indicate that treatment of the plant
with the peptide miPEP165a leads to an increase in the accumulation
of miR165a (FIG. 25).
[0809] Moreover, to determinate the mode of action of miPEPs
regarding the accumulation of their own miRs, the expression of
pri-miR165a has been analysed in wildtype plants and in plants that
have been transformed to overexpress miPEP165a (FIG. 35).
[0810] The overexpression of pri-miR165a is enhanced in the plants
that overexpress miPEP165a (FIG. 36A).
[0811] In the presence of cordycepin, an inhibitor of RNA
synthesis, the amount of pri-miR165a is identical in wildtype
plants and in transformed plants (FIG. 36B), it indicates that
miPEP165a does not operate as a RNA stabilizer, but rather as a
transcription activator of pri-miR165a.
[0812] Moreover, the expression of pri-miR165a has been analysed in
wildtype plants (Col-0 plants) and in mutated plants having a low
allele of the second large subunit of RNA polymerase II (nrpb2-3
plants). The results obtained indicate that mutated plants do not
show an increase in the accumulation of pri-miR165 in response to
miPEP165a, unlike wildtype plants. It appears that the miPEP
operates as a transcription regulator.
Example 3D
AtmiPEP319a1
[0813] Regarding AtmiPEP319a1, this was also synthesized and was
used for investigating an increase in the accumulation of miR319a
in roots of A. thaliana treated with the synthetic peptide.
[0814] Analyses by qRT-PCR show that the overexpression of
AtmiPEP319a1 leads to an increase in the accumulation of miR319a
(FIGS. 26 and 31).
[0815] It was also demonstrated in vivo in Arabidopsis thaliana
that treatment of the plant with AtmiPEP319a1 increases plant
growth significantly (FIG. 27).
Example 3E
AtmiPEP160b1
[0816] Regarding AtmiPEP160b1, it has been demonstrated in vivo in
A. thaliana that the overexpression of this miPEP induces an
accumulation of AtmiR160b (FIG. 29).
[0817] Material and Methods
[0818] Biological Material
[0819] The surface of seeds of M truncatula was sterilized and they
were left to germinate on agar plates for 5 days at 4.degree. C. in
the dark. The young shoots were then grown on 12-cm square plates
filled with Fahraeus medium without nitrogen and containing 7.5
.mu.M phosphate (Lauressergues et al., Plant J., 72(3): 512-22,
2012). The lateral roots were counted every day. In pots, the
plants were watered every other day with modified Long Ashton
medium with low phosphorus content (Balzergue et al., Journal of
Experimental Botany, (62)1049-1060, 2011).
[0820] The peptides were synthesized by Eurogentec or
Smartox-Biotech. MtmiPEP171b1 was resuspended in a solution of 40%
water/50% acetonitrile/10% acetic acid (v/v/v), and the other
peptides were resuspended in water.
[0821] The leaves were watered by spraying with the peptides using
peptide solutions at different concentrations (0.01, 0.1, 1 .mu.M),
firstly 12 h before sampling and then a second time 30 min before
sampling.
[0822] Reverse Transcription of the microRNAs
[0823] The RNA was extracted using the reagent Tri-Reagent (MRC)
according to the manufacturer's instructions, except for
precipitation of the RNA, which was carried out with 3 volumes of
ethanol. The everse transcription of the RNA was carried out using
the specific stem-loop primer RTprimer171b in combination with
hexamers for performing the reverse transcription of RNA of high
molecular weight.
[0824] In brief, 1 .mu.g of RNA was added to the stem-loop primer
MIR171b (0.2 .mu.M), the hexamer (500 ng), the buffer RT
(1.times.), the enzyme SuperScript Reverse transcriptase (SSIII)
(one unit), the dNTPs (0.2 mM each), DTT (0.8 mM) in a final
reaction mixture of 25 .mu.l. In order to carry out the reverse
transcription, a reaction of pulsed reverse transcription was
performed (40 repetitions of the following cycle: 16.degree. C. for
2 minutes, 42.degree. C. for one minute and 50.degree. C. for one
second, followed by a final inactivation of the reverse
transcription at 85.degree. C. for 5 minutes).
[0825] Analyses by Quantitative RT-PCR (qRT-PCR)
[0826] The total RNA was extracted from roots of M. truncatula or
from tobacco leaves using the extraction kit RNeasy Plant Mini Kit
(Qiagen). The reverse transcription was performed using the reverse
transcriptase SuperScript II (Invitrogen) starting from 500 ng of
total RNA. Three repetitions (n=3) were carried out, each with two
technical repetitions. Each experiment was repeated from two to
three times. The amplifications by qPCR were carried out using a
LightCycler 480 System thermocycler (Roche Diagnostics) by the
method described in Lauressergues et al. (Plant J., 72(3): 512-22,
2012).
[0827] Statistical Analyses
[0828] The mean values of the relative expression of the genes or
of the production of lateral roots were analysed using the Student
test or the Kruskal-Wallis test. The error bars represent the SEM
(Standard Error of the Mean). The asterisks indicate a significant
difference (p<0.05).
[0829] Plasmid Constructs
[0830] The DNA fragments of interest were amplified with Pfu
polymerase (Promega). The DNA fragments were cloned using the XhoI
and NotI enzymes into a pPEX-DsRED plasmid for an overexpression
under the control of the constitutive strong promoter 35S, and
using the KpnI-NcoI enzymes into a pPEX GUS plasmid for the
reporter genes, by the method described in Combier et al. (Genes
& Dev, 22: 1549-1559, 2008).
[0831] For the miPEPs 165a and 319a, the corresponding miORFs were
cloned into pBIN19 by the method described in Combier et al. (Genes
& Dev, 22: 1549-1559, 2008).
[0832] Transformation of the Plants
[0833] The composite plants having roots transformed with
Agrobacterium rhizogenes were obtained by the method described in
Boisson-Dernier et al. (Mol Plant-Microbe Interact, 18: 1269-1276,
2005). The transformed roots were verified and selected by
observations of DsRED with a binocular fluorescence magnifier. The
control roots correspond to roots transformed with A. rhizogenes
not containing the pPEX-DsRED vector. Transformation of the tobacco
leaves was carried out by the method described in Combier et al.
(Genes & Dev, 22: 1549-1559, 2008).
[0834] Northern Blot
[0835] Northern blot analysis was carried out according to the
protocol described in Lauressergues et al. Plant J., 72(3): 512-22,
2012.
[0836] The biological samples were homogenized in a buffer
containing 0.1 M of NaCl, 2% of SDS, 50 mM of Tris-HCl (pH 9), 10
mM of EDTA (pH 8) and 20 mM of mercaptoethanol, and the RNA was
extracted twice with a phenol/chloroform mixture and was
precipitated with ethanol.
[0837] The RNA was loaded on PAGE 15% gel and transferred to a
nylon membrane (HybondNX, Amersham). RNA was hybridized with a
radioactive oligonucleotide probe labelled at its end, in order to
detect the RNA U6 or for miR164a.
[0838] The hybridizations were carried out at 55.degree. C. The
hybridization signals were quantified using a phosphorimager (Fuji)
and normalized with the signal of the specific probe of RNA U6.
[0839] RNA Stability
[0840] For each condition, two week old plants that have been
cultivated vertically on solid MS medium have been transferred in
6-well plates containing 1 ml of liquid MS medium. After a 16 hour
incubation with 1 mM of miPEP, the plants have been treated with
100 .mu.g/ml of cordycepin or with water (as control), and
harvested a different times to extract and quantify RNA. Each
indivual experiment has been made 3 times.
[0841] Histochemical Labelling
[0842] Labelling with GUS was carried out by the method described
in Combier et al., (Genes & Dev, 22: 1549-1559, 2008). The
samples were observed with a microscope (axiozoom).
[0843] Immunolocalization
[0844] Roots or plantlets of tissues of Medicago were fixed for 2
hours in 4% formol (v/v) with 50 mM of phosphate buffer (pH 7.2),
and then embedded in agarose LMP 5% in water (with a low melting
point). Thin sections (100 .mu.m) were obtained and were placed in
Pbi (phosphate buffer for immunology) on Teflon-coated slides,
blocked in Pbi, 2% Tween and 1% of bovine serum albumin for 2 hours
(PbiT-BSA), then labelled overnight (12 h) at 4.degree. C. with the
primary antibody diluted in BSA-PbiT. The sections were washed with
PBiT and incubated at ambient temperature for 2 h with a secondary
antibody diluted in PbiT-BSA. The slides were then washed in Pbi
for 30 min and mounted in Citifluor (mounting medium). The primary
antibodies and the dilutions were as follows: 1716a (1:500, v/v).
The secondary antibody was a goat anti-rabbit IgG antibody coupled
to the Alexa Fluor 633 fluorescent probe (Molecular Probes), and
was used at a dilution of 1:1000 (v/v).
[0845] Western Blotting
[0846] A total extract of proteins has been prepared using the
method described in Combier et al., (Genes & Dev, 22:
1549-1559, 2008) and separated by SDS-PAGE. The transfer has been
made using a phosphate buffer overnight at 4.degree. C., at 15V,
then the membrane has been incubated for 45 min at room temperature
in a solution of glutaraldehyde 0.2% (v/v). Primary antibodies have
been used at a 1:1000 (v/v) dilution and HRP-conjugated goat
anti-rabbit Ig antibodies have used as secondary antibodies at a
1:40 000 (v/v) dilution.
TABLE-US-00008 TABLE 8 List of primers AtmiR160bq5 (SEQ ID NO: 429)
TCCCCAAATTCTTGACCAAA AtmiR160bq3 (SEQ ID NO: 430)
TTGAGGGGAAAACATGAACC AtmiR164aq5 (SEQ ID NO: 431)
ACGAAATCCGTCTCATTTGC AtmiR164aq3 (SEQ ID NO: 432)
GGGTGAAGAGCTCATGTTGG AtmiR165q5 (SEQ ID NO: 433)
TGAGGGGAATGTTGTCTGG AtmiR165q3 (SEQ ID NO: 434) GAAGCCTGGTCCGAGGATA
AtmiR319q5 (SEQ ID NO: 435) CGAGTCGCCAAAATTCAAAC AtmiR319q3 (SEQ ID
NO: 436) GCTCCCTTCAGTCCAATCAA AtPHVq5 (SEQ ID NO: 437)
GCAACTGCAGTGGAATAGCA AtPHVq3 (SEQ ID NO: 438) GCGACCTTCATGGGTTCTAA
AtPHBq5 (SEQ ID NO: 439) CTCAGCATCAGCAACGTGAT AtPHBq3 (SEQ ID NO:
440) AACTCTGCTAGGGCCTCCTC AtREVq5 (SEQ ID NO: 441)
TCACAACTCCTCAGCATTCG AtREVq3 (SEQ ID NO: 442) ACCCAATCAACAGCAGTTCC
Atactine2F (SEQ ID NO: 443) GGTAACATTGTGCTCAGTGG Atactine2R (SEQ ID
NO: 444) CTCGGCCTTGGAGATCCACA miORF319acla5 (SEQ ID NO: 445)
tcatcgATGAATATACATACATACCATCAT miORF319abam3 (SEQ ID NO: 446)
tccggatccTCTAAAAGCTAAAGTGATTCTAAA AtmiR319cla5 (SEQ ID NO: 447)
tcatcgAGAGAGAGCTTCCTTGAGTC AtmiR319bam3 (SEQ ID NO: 448)
tccggatccAGAGGGAGCTCCCTTCAGT AtmiR165cla5 (SEQ ID NO: 449)
tcatcgGTTGAGGGGAATGTTGTCTG AtmiR165bam3 (SEQ ID NO: 450)
tccggatccGTTGGGGGGGATGAAGCC AtmiR165muorf5cla (SEQ ID NO: 451)
tcatcgATGAGGGTTAAGCTATTTCAGT AtmiR165muorf3bam (SEQ ID NO: 452)
tccggatccTAATATCCTCGATCCAGACAAC miR171bq5 (SEQ ID NO: 453)
CGCCTCAATTTGAATACATGG miR171bq3 (SEQ ID NO: 454)
ACGCGGCTCAATCAAACTAC pri-miR171bq5 (SEQ ID NO: 455)
GCACTAGCTGGTTTCATTATTCC pri-miR171bq3 (SEQ ID NO: 456)
TTGCAAAATTTGGAGAGCCTA miR171eq5 (SEQ ID NO: 457)
AAGCGATGTTGGTGAGGTTC miR171eq3 (SEQ ID NO: 458)
CGGCTCAATCTGAGATCGTAT miR169dq5 (SEQ ID NO: 459)
GGGCGGTGAGATTAACAAAA miR169dq3 (SEQ ID NO: 460)
CCTGCCGGAAATGAAACTAA miORF171e2Cla5 (SEQ ID NO: 461)
tcatcgATGATGGTGTTTGGGAAGCC miORF171e2bam3(SEQ ID NO: 462)
tccggatccTACATGTAAATCCGTCTTCGG miORF171b5'AGG (SEQ ID NO: 463)
AGGCTTCTTCATAGGCTCTCC miR171e5'Xho (SEQ ID NO: 464)
TCAGTCCGCTCGAGGAATAAGTGAATAT TATCGATATTT miR171e3'Not (SEQ ID NO:
465) AAGGAAAAAAGCGGCCGCAAAGTGATA TTGGCGCGGCT 171b scramble F bis
(SEQ ID NO: 466) TAGCATGTTATTACACCGCTTAAGTAAG
TTCTGTAAGATAGAGCGCGATATTGTTT ACATTAGCTAG 171b scramble R bis (SEQ
ID NO: 467) CGTACTAGCTAATGTAAACAATATCGCG
CTCTATCTTACAGAACTTACTTAAGCGG TGTAATAACAT PromiORF171b1 ATG BsaIR
aaaaGGTCTCgCCATAAGCATAGTGGAAT (SEQ ID NO: 468) AATGAAACC
PromiORF171b2 ATG BsaIR aaaaGGTCTCgCCATTTCCATACCTGAATT (SEQ ID NO:
469) TCTCCTT PromiORF171b6 ATG BsaI R
aaaaGGTCTCgCCATCTTCATCCTCAATAT (SEQ ID NO: 470) CCTAATTC 171b
fusion GFP sans ATG BsaI aGGTCTCgACACAGATATATATACTATGT R (SEQ ID
NO: 471) CTCTTTCAATTTTGC Pro171b fusionTranscript GUSpPEX
aaaGGTCTCgCCATGACCATTTTCCCACG BsaI R (SEQ ID NO: 472) AATATG
Pro171b fusionATG1 aaGGTCTCgACACAAGCATAGTGGAATA GUSpPEXsansATG BsaI
R ATGAAACC (SEQ ID NO: 473) Pro171bfusionTraducGUSpPEX
aaGGTCTCgACACAGATATATATACTATG BsaI R (SEQ ID NO: 474) TCTCTTTCA
171b NcoI F (SEQ ID NO: 475) Gaattcctgcccatggttttcg 171b NcoI R
(SEQ ID NO: 476) Cgaaaaccatgggcaggaattc 171b AflII F (SEQ ID NO:
477) cagtcccgacttaagctcaatac 171b AflII R (SEQ ID NO: 478)
gtattgagcttaagtcgggactg 171b genomiq BsaI F aaaGGTCTCCTAGC (SEQ ID
NO: 479) TTGGTCAAACATACATACAGTAGCACTAG 171b genomiq BsaI R
aaaGGTCTCgCGTAAAATTGTACTGATTG (SEQ ID NO: 480) AATGTAAATTGGTACAC
AthmiORF160b F (SEQ ID NO: 481) TAGCATGTTTTCCCCTCAATGA AthmiORF160b
R (SEQ ID NO: 482) cgtaTCATTGAGGGGAAAACAT AthMIR160b BsaI F (SEQ ID
NO: 483) aaaGGTCTCcTAGCACTCATAACTCTCCCC AAATTC AthMIR160b BsaI R
(SEQ ID NO: 484) aaaGGTCTCgCGTAGAAAGAATGTTCGAA AAACAATG pro 165a
BsaI F (SEQ ID NO: 485) aGGTCTCCAAATcatgaagcaggcagtaataacct 165a
muORF tagHA BsaI R aGGTCTCgCGTActaAGCGTAATCTGGAAC (SEQ ID NO: 486)
ATCGTATGGGTAtaatatcctcgatccagacaac 165a fusion transcript BsaI R
aGGTCTCgccatagtggcggagacgaagatg (SEQ ID NO: 487) 165a fusion traduc
BsaI R aGGTCTCgccattaatatcctcgatccagacaac (SEQ ID NO: 488) pro 165a
BsaI F (SEQ ID NO: 489) aGGTCTCCAAATtaaactgtcagtgcatggatgt 165a
fusion GTG BsaI R aGGTCTCgACACattcacaaagttgtagagag (SEQ ID NO: 490)
165a fusion GTGmutGTT BsaI aGGTCTCgACACattaacaaagttgtagagag R (SEQ
ID NO: 491) 165a fusion CTG BsaI R
aGGTCTCgACACtagcagattcacaaatttttgttg (SEQ ID NO: 492) 165a fusion
ATG BsaI R aGGTCTCgACACcctcatgataatcgatcttagca (SEQ ID NO: 493)
[0847] B: Analysis of the miPEPs in Animals
Example 4
Identification of Candidate miPEPs in Drosophila
[0848] a) Identification of miPEP Candidates
[0849] A first study carried out by RACE-PCR in the model animal
Drosophila melanogaster shows the existence of miRNAs that are
expressed during embryogenesis, miR1 and miR8.
[0850] As in the plants, miORFs were identified in each of the two
miRNAs studied. For example, miR8, known for its role in the
regulation of growth in insects, has a miORF potentially encoding
miPEP8.
[0851] Regarding DmmiR1 (identified in Drosophila melanogaster), it
has two DmmiORFs potentially encoding DmmiPEPla and DmmiPEP1b.
[0852] A phylogenetic analysis shows evolutionary conservation of
the presence of the miORFs among the dozen Drosophila species
analysed, i.e. since their divergence more than 60 million years
ago (FIG. 13).
[0853] Moreover, the miPEPs identified in Drosophila have several
similarities with the plant miPEPs. If their primary sequence and
therefore their size evolve rapidly between species, a reduced size
(from 32 to 104 AA) is found, as well as strong conservation for a
basic overall charge (pHi from 9.5 to 12) (FIG. 14).
[0854] Taken together, these results therefore indicate the
existence of regulatory miPEPs, encoded by the primary transcript
of the microRNAs, over a broad spectrum of eukaryotic species.
These discovered peptides represent an as yet unexplored reservoir
of natural molecules that may regulate a variety of fundamental
biological functions, both in plants and in animals.
[0855] b) Expression of the miPEPs
[0856] Drosophila melanogaster cells have been transfected to
overexpress the DmmiPEP8 (from the sequence SEQ ID NO: 494) or the
DmmiPEP mt of which start codons have been mutated (from the
sequence SEQ ID NO: 495). As a transfection control, one
moesin-RFP-producing plasmid has been co-transfected with the
plasmids encoding DmmiPEP8 and DmmiPEP8 mt. It is observed that
start codons in the DmmiPEP8 sequence are functional because they
allow the synthesis of a miPEP::GFP fusion protein shown by the GFP
whereas the constructions with mutated ATGs do not produce hardly
any GFP.
[0857] These results suggest that the ORF of the miPEP is
functional (FIG. 38).
[0858] Material and Methods
[0859] Cells of Drosophila melanogaster
[0860] S2 cells are cultured in a T75 flask in 12 mL of Schneider's
medium (GIBCO), containing 1% of penicillin 100 U/mL and
streptavidin 100 mg/mL (Sigma) and 10% of decomplemented foetal
calf serum (30 min at 56.degree. C.).
[0861] The transient transfections are carried out using the
FuGENE.RTM. HD transfection kit (Roche), according to the
recommendations. Conventionally, 1.5 million S2 cells, previously
seeded in 6-well plates (3 ml of medium per well), are transfected
with 250 ng of total plasmid DNA. The DNA is brought into contact
with the Fugene (3 .mu.l) in 100 .mu.l of OPTIMEM (GIBCO). After 20
minutes, the transfection reagent formed is brought into contact
with the cells in the culture medium. The RNA of the cells is
extracted 66 h after transfection.
[0862] Cloning of DmmiPEP8- and Mutated DmmiPEP8-Fragments
[0863] The DNA fragments, corresponding to the sequences of
DmmiPEP8 (SEQ ID NO: 494) and of DmmiPEP8 mutated in the start
codons (SEQ ID NO: 495), have been amplified by PCRin order to be
cloned in accordance with the GFP in a pUAS plasmid. The SE insect
cells have been co-transfected with a mix of plasmids (1/1/1 ratio,
a total of 300 ng) encoding DmmiPEP8::GFP or its mutated form
DmmiPEP8 mt::GFP, a pACT-GAL4 allowing their overexpression by
means of a GAL4 transcription factor produced under the control of
the actin promotor and a expression vector encoding the moesin-RFP
protein used as a transfection control, also under the control of
the actin promotor. 48 h after transfection, the cells are fixed by
the addition of formaldehyde in the final medium culture 4% (WN)
for 10 min. Then, the cells are washed 2.times. with PBS 1X and
coated on slides in order to be observed under a microscope (Leica
SPE).
TABLE-US-00009 TABLE 9 Cloned sequences.
CGCACACACACACACACACAATCACTCACACGCGGTCACAC SEQ ID
GCACATTTCAATAAACTAATGGAGCCTGGCTTTGTTTTTGTT NO: 494
TTATTTCCAACCCACTTGAGCACACAGCACACACAGAGAGA
AAAATCAATACTCGTTATGGGATTAAATTTACAAAGCGCAA
AGCAAAGCGACAAACAAAATTCAAAAGAAAGAAAAAAAAA
CACTCAAATAAACTCACAAAGAATTCCTTATCGCCAAGGGG
GCCAATGTTCTAAGGTTCTTTCGCCT
CGCACACACACACACACACAATCACTCACACGCGGTCACAC SEQ ID
GCACATTTCAATAAACTAAGTGAGCCTGGCTTTGTTTTTGTT NO: 495
TTATTTCCAACCCACTTAAGCACACAGCACACACAGAGAGA
AAAATCAATACTCGTTAGTGGATTAAATTTACAAAGCGCAA
AGCAAAGCGACAAACAAAATTCAAAAGAAAGAAAAAAAAA
CACTCAAATAAACTCACAAAGAATTCCTTATCGCCAAGGGG
GCCAATGTTCTAAGGTTCTTTCGCCT
[0864] C: Characterization of miPEPs in Humans
Example 5
Characterization of HsmiPEP155
[0865] The DNA fragments of interest (HsmiPEP155 and the mutated
miPEP) were synthesized or amplified by PCR using specific primers,
and then cloned using the enzymes XhoI and NotI into a pUAS plasmid
permitting their overexpression by means of the GAL4 transcription
factor, the expression of which is controlled by a constitutive
strong promoter.
[0866] The different constructs were produced either by PCR
amplification on genomic DNA of HeLa cells, or by RT-PCR on total
RNAs of L428 human cells. The amplified PCR fragments are digested
with the HindIII/EcoRI restriction enzymes and then cloned into the
vector pcDNA3.1. The DH5.alpha. strain of Escherichia coli is
electroporated and then cultured on a solid medium
(2YT+agar+ampicillin). The plasmid DNA from different clones is
then prepared and sequenced for verification. The constructs are
then prepared using the QIAfilter Plasmid Midi kit (QIAGEN) and
stored at -20.degree. C.
[0867] The HeLa cells (established tumour line, ATCC CCL-2.2) are
cultured in a 6-well plate in complete medium [(DMEM
(1x)+Glutamax+4.5 g/L glucose without pyruvate+1x
penicillin/streptomycin+1 mM Na-pyruvate+10% calf serum] and placed
in an incubator at 37.degree. C. and 5% CO.sub.2.
[0868] The cells are transfected when they are at 50% confluence.
At the start of the experiment, the complete medium containing the
antibiotics is replaced with complete medium without
antibiotics.
[0869] For each well, a mix A [250 .mu.l of Optimem (+Glutamax)
(Gibco)+2 .mu.g of DNA] and a mix B [250 .mu.l of Optimem+4 .mu.l
of Lipofectamine 2000 (Invitrogen)] is prepared, and left for 5 min
at ambient temperature. Then mix B is mixed dropwise into mix A,
and left to incubate for 25 min at ambient temperature. The mixture
is then deposited dropwise into the well. 4-5 hours later, the
medium is changed and replaced with complete medium with
antibiotics. 48 hours after transfection, the cells are stopped.
The medium is aspirated and discarded; the cells are rinsed with
PBS 1.times.. It is then possible to store the cells at -20.degree.
C. or extract the total RNAs directly.
[0870] For each well, the RNAs are extracted by depositing 1 ml of
Tri-Reagent (Euromedex) on the cells. The Tri-reagent is aspirated
and returned several times so that the cells are lysed correctly,
and then it is transferred into a 1.5-ml tube. 0.2 ml of
water-saturated chloroform is added. It is mixed by vortexing, then
left for 2 to 3 minutes at ambient temperature. It is centrifuged
for 5 minutes at 15300 rpm and at 4.degree. C. The aqueous phase is
precipitated from 0.5 ml of isopropanol after incubation for 10
minutes at ambient temperature and centrifugation for 15 minutes at
15300 rpm and at 4.degree. C. The supernatant is discarded and the
pellet is rinsed with 1 ml of 70% ethanol, with centrifugation for
5 minutes at 15300 rpm at 4.degree. C. The supernatant is again
discarded and the pellet is dried for a few minutes in the air. For
best-possible removal of the genomic DNA potentially remaining, the
RNAs are treated with DNase. For this, the pellet is resuspended in
170 .mu.l of ultra-pure water, 20 .mu.l of DNase buffer 10.times.
and 10 .mu.l of RQ1 RNase-free DNase and held at 37.degree. C. for
30 minutes. Then 20 .mu.l of SDS10% and 5 .mu.l of proteinase K (20
mg/ml) are added over 20 minutes at 37.degree. C.
[0871] A last phenol extraction is carried out with 225 .mu.l of a
phenol/H.sub.2O/chloroform mixture, and centrifuged for 5 minutes
at 15300 rpm at 4.degree. C.
[0872] The aqueous phase is then precipitated from 20 .mu.l of 3M
sodium acetate and 600 .mu.l of 100% ethanol for 20 minutes at
-80.degree. C. Then it is centrifuged for 15 min at 4.degree. C. at
15300 rpm. The supernatant is discarded. The pellet is rinsed in 1
ml of 70% ethanol, centrifuged for 5 min at 15300 rpm at 4.degree.
C., the supernatant is discarded again and the pellet is left to
dry for some minutes in the air.
[0873] The pellet is then taken up in 15-20 .mu.l of ultra-pure
water and the RNAs are assayed.
[0874] 10-15 .mu.g of total RNAs is then analysed by Northern blot
on 15% acrylamide gel [solution of acrylamide/40% bis-acrylamide,
ratio 19:1], 7M urea in TBE 1x. Migration is carried out at 400V,
in TBE1x as migration buffer, after preheating the gel. The RNAs
are then electro-transferred onto a Biodyne Plus 0.45 .mu.m nylon
membrane, for 2 hours, at 1V and 4.degree. C. in a transfer tank.
At the end of transfer, the membrane is irradiated with UV at 0.124
J/cm.sup.2. The membrane is then pre-hybridized in a buffer
5.times. SSPE, 1.times. Denhardt's, 1% SDS and 150 .mu.g/ml of
yeast tRNA, for 1 hour at 50.degree. C. in a hybridization oven.
Then the nucleotide probe is added, labelled at 5' with
.gamma.-.sup.32P-ATP (0.5 to 110.sup.6 cpm/ml of hybridization
buffer) and is hybridized overnight at 50.degree. C. The membrane
is then washed twice in 0.1.times. SSPE/0.1% SDS at ambient
temperature and exposed in an autoradiography cassette containing a
BioMax HE screen (Kodak) and a BioMax MS film (Kodak), in order to
detect a microRNA, for 24-48 hours, at -80.degree. C.
Sequence CWU 1
1
495133PRTArabidopsis thaliana 1Met Phe Cys Ser Ile Gln Cys Val Ala
Arg His Leu Phe Pro Leu His 1 5 10 15 Val Arg Glu Ile Lys Lys Ala
Thr Arg Ala Ile Lys Lys Gly Lys Thr 20 25 30 Leu 224PRTArabidopsis
thaliana 2Met Arg Arg Gln Thr Ser Val Pro Phe Ala Cys Lys Arg Asp
Lys Glu 1 5 10 15 Ser Asp Lys Ser His Lys Glu Arg 20
337PRTArabidopsis thaliana 3Met Val Met Phe Phe Leu Asp Leu Asp Lys
Asn Pro Arg Phe Asp Leu 1 5 10 15 Leu Lys Gly Leu Lys Trp Asn Leu
Phe Ser Ser His Ile Ser Pro Ser 20 25 30 Leu Pro Pro Ser Leu 35
411PRTArabidopsis thaliana 4Met Lys Asp Asn Phe Pro Leu Leu Leu Arg
Leu 1 5 10 54PRTArabidopsis thaliana 5Met Ser Asp Asp 1
616PRTArabidopsis thaliana 6Met Ile Tyr Ile Asn Lys Tyr Gly Ser Ile
Ser Ala Val Glu Asp Asp 1 5 10 15 74PRTArabidopsis thaliana 7Met
Ser Gln Arg 1 816PRTArabidopsis lyrata 8Met Thr Cys Pro Leu Leu Ser
Leu Ser Phe Leu Leu Ser Lys Tyr Ile 1 5 10 15 916PRTArabidopsis
thaliana 9Met Thr Trp Pro Leu Leu Ser Leu Ser Phe Leu Leu Ser Lys
Tyr Val 1 5 10 15 1018PRTCapsella rubella 10Met Thr Cys Thr Leu Ser
Ala Leu Ser Leu Ser Leu Asn Met Phe Arg 1 5 10 15 Val Asn
115PRTArabidopsis thaliana 11Met Phe Tyr Leu Ser 1 5
1237PRTArabidopsis thaliana 12Met Val Asn Thr Ser Ser Phe Phe Ile
Ser Ser Phe Ile Leu Pro Leu 1 5 10 15 Val Leu Ser Glu Ser Asn Cys
Leu Leu Phe Arg Thr Ile Tyr Lys Phe 20 25 30 Ser Met Val Leu Tyr 35
1324PRTArabidopsis thaliana 13Met Phe Cys Leu Leu Ile Pro Ile Phe
Ser Phe Val Phe Ser Pro Asn 1 5 10 15 Arg His Leu Arg Leu Gln Glu
Gln 20 145PRTArabidopsis thaliana 14Met Phe Ser Pro Gln 1 5
1523PRTArabidopsis thaliana 15Met Lys Tyr Ile His Ile Leu Ile Leu
Phe Lys Ser Arg Ser Thr Tyr 1 5 10 15 Lys Leu Ser Thr Asn His Ile
20 1610PRTArabidopsis thaliana 16Met Lys Ile Pro Leu Phe Leu Pro
Lys Leu 1 5 10 1727PRTArabidopsis thaliana 17Met Val Ser Gly Gln
Glu Asp Ser Trp Leu Lys Leu Ser Ser Leu Cys 1 5 10 15 Phe Leu Phe
Leu Ser Leu Leu Asp Ser Leu Ile 20 25 1834PRTArabidopsis thaliana
18Met Phe Leu Leu Ile Phe Leu Arg Leu Ile Met Ile Cys Val Cys Ser 1
5 10 15 Ser Thr Asp Phe Leu Arg Ser Val Asn Tyr Phe Cys Leu Phe Ile
Tyr 20 25 30 Asp Leu 199PRTArabidopsis thaliana 19Met Ser Thr Thr
Gln Glu His Arg Ser 1 5 2025PRTArabidopsis thaliana 20Met Ile Leu
Lys Cys Trp Ser Ser Arg Phe Leu Arg Val Ser Pro Tyr 1 5 10 15 Gln
Asn Ala His Ser Leu Ser Leu Gly 20 25 2113PRTArabidopsis lyrata
21Met Pro Leu Ala Val Ile Arg Gln Gly Ile Val Trp Pro 1 5 10
2224PRTArabidopsis lyrata 22Met Pro Ser Trp His Asp Met Val Leu Leu
Pro Tyr Val Lys His Thr 1 5 10 15 His Ala Asn Thr Arg His Ile Thr
20 238PRTArabidopsis lyrata 23Met Thr Trp Phe Phe Cys Leu Thr 1 5
2437PRTArabidopsis thaliana 24Met Pro Ser Trp His Gly Met Val Leu
Leu Pro Tyr Val Lys His Thr 1 5 10 15 His Ala Ser Thr His Thr His
Thr His Asn Ile Tyr Gly Cys Ala Cys 20 25 30 Glu Leu Val Phe His 35
2522PRTArabidopsis thaliana 25Met Ala Trp Tyr Gly Ser Phe Ala Leu
Arg Lys Thr His Ser Arg Gln 1 5 10 15 His Thr His Thr His Thr 20
268PRTArabidopsis thaliana 26Met Val Trp Phe Phe Cys Leu Thr 1 5
277PRTBrassica rapa 27Met Met Ile Ile Leu Trp Lys 1 5
2824PRTBrassica rapa 28Met Leu Trp Ala Lys Leu Val Ser Phe Ser Thr
Leu His Ser Leu Val 1 5 10 15 Phe Leu Leu Ser Pro Ser Phe Ala 20
2929PRTBrassica rapa 29Met Pro Ser Trp His Gly Ile Val Ile Leu Pro
Phe Val Lys His Thr 1 5 10 15 His Ala Asn Ile His Tyr Ser Tyr Ser
Cys Val Cys Ile 20 25 3034PRTCarica papaya 30Met Ile Ala Cys His
Pro Tyr Leu Pro Phe Pro Leu Phe Leu Ser Leu 1 5 10 15 Thr Phe Tyr
Ser Ile Phe Phe Ser Pro Ser Pro Pro Ser Pro Ser Leu 20 25 30 Pro
Leu 3153PRTCarica papaya 31Met Pro Ser Leu Leu Ala Phe Ser Pro Phe
Pro Phe Ser Asn Ile Leu 1 5 10 15 Leu Asn Leu Leu Leu Pro Leu Pro
Pro Phe Pro Leu Ser Ala Ile Ile 20 25 30 Thr Ile Ile Lys Pro Leu
Ser Leu Ser Leu Pro Leu Ser Leu Ser Leu 35 40 45 Ser Gly Phe Ser
Val 50 3215PRTCapsella rubella 32Met Glu Leu Lys Gly Leu Arg Thr
Trp Gln Leu Leu Asp Lys Val 1 5 10 15 3314PRTCapsella rubella 33Met
Pro Ser Trp His Gly Met Ala Cys Phe Tyr Cys Leu Thr 1 5 10
3424PRTCapsella rubella 34Met Ala Trp His Gly Met Phe Leu Leu Pro
Tyr Val Lys His Thr His 1 5 10 15 Ala Asn Thr Tyr Ser Leu Tyr Met
20 3535PRTGossypium raimondii 35Met Met Arg Ser Arg Ile Leu Lys Phe
Gln Tyr Arg Phe Gly Met Gly 1 5 10 15 Ile Gly Gly Arg Lys Gln Leu
Lys Asn Gln Leu Cys Gln Ile Gln Gly 20 25 30 Arg Ile Ser 35
3610PRTGossypium raimondii 36Met Ser Asn Ser Arg Ser Tyr Gln Leu
Lys 1 5 10 3717PRTGossypium raimondii 37Met Asn Glu Asp Leu Glu Ile
Ser Thr Arg Lys Arg Thr Pro Gln Leu 1 5 10 15 Cys 3812PRTMedicago
truncatula 38Met Pro Lys Phe Asp Ile Phe Phe Tyr Ile Phe Val 1 5 10
3983PRTMedicago truncatula 39Met Ser Tyr Ile Ser Leu Ser Pro Lys
Leu Leu Pro Ile Asn Thr Lys 1 5 10 15 Pro Phe Pro Trp Leu Val Gln
Phe Asn Phe Tyr Phe Ser Ser Asn Thr 20 25 30 Lys Cys Asn Lys Leu
His Phe Leu Gly Glu Lys Leu Leu Val Gly Glu 35 40 45 Ala Gly His
Val Gln Ile Leu Phe Leu Ile His Ser Leu Ile Met His 50 55 60 Ile
Asn Ile Phe Cys Thr Cys Ser Pro Ser Pro Thr Arg Leu Pro His 65 70
75 80 Pro Ser Leu 4020PRTOryza sativa 40Met Gln Thr His Ser Asn Thr
Pro Gln Ser Thr Tyr Ser Leu Ser Leu 1 5 10 15 Ser Leu Ser Glu 20
4115PRTOryza sativa 41Met Cys Val Cys Asp Ile Asn Met His Ser Met
Leu Met Leu Leu 1 5 10 15 4225PRTArabidopsis lyrata 42Met Arg Ile
Lys Leu Phe Gln Leu Arg Gly Met Leu Ser Gly Ser Arg 1 5 10 15 Ile
Leu Tyr Ile Tyr Thr Cys Val Cys 20 25 4318PRTArabidopsis thaliana
43Met Arg Val Lys Leu Phe Gln Leu Arg Gly Met Leu Ser Gly Ser Arg 1
5 10 15 Ile Leu 4436PRTBrassica carinata 44Met Arg Met Lys Leu Phe
Gln Leu Arg Gly Met Leu Ser Gly Ser Arg 1 5 10 15 Ile Leu Tyr Ile
His Lys Tyr Val Tyr Met Leu Ile Gln Val Phe Asp 20 25 30 His Ile
Cys Ile 35 4527PRTBrassica juncea 45Met Arg Met Lys Leu Phe Gln Leu
Arg Gly Met Leu Ser Gly Ser Arg 1 5 10 15 Ile Leu Tyr Ile His Lys
Tyr Val Tyr Ile Cys 20 25 4636PRTBrassica napus 46Met Arg Met Lys
Leu Phe Gln Leu Arg Gly Met Leu Ser Gly Ser Arg 1 5 10 15 Ile Leu
Tyr Ile His Lys Tyr Val Tyr Met Ile Ile Gln Val Phe Asp 20 25 30
His Ile Cys Ile 35 4736PRTBrassica oleracea 47Met Arg Met Lys Leu
Phe Gln Leu Arg Gly Met Leu Ser Gly Ser Arg 1 5 10 15 Ile Leu Tyr
Ile His Lys Tyr Val Tyr Met Leu Ile Gln Val Phe Asp 20 25 30 His
Ile Cys Ile 35 4827PRTBrassica rapa 48Met Arg Met Lys Leu Phe Gln
Leu Arg Gly Met Leu Ser Gly Ser Arg 1 5 10 15 Ile Leu Tyr Ile His
Lys Tyr Val Tyr Ile Cys 20 25 4919PRTArabidopsis thaliana 49Met Leu
Asp Leu Phe Arg Ser Asn Asn Arg Ile Glu Pro Ser Asp Phe 1 5 10 15
Arg Phe Asp 504PRTArabidopsis thaliana 50Met Arg Asp Arg 1
5110PRTArabidopsis thaliana 51Met Asn Arg Lys Ile Ser Leu Ser Leu
Ser 1 5 10 528PRTArabidopsis thaliana 52Met Met Gly Cys Phe Val Gly
Phe 1 5 538PRTArabidopsis thaliana 53Met Gln Glu Glu Thr Tyr Glu
Gly 1 5 5459PRTArabidopsis thaliana 54Met Pro His Thr Asn Leu Lys
Asp Leu Phe Ile Phe Ser Pro Asn Val 1 5 10 15 Phe Phe Ser Phe Ala
Ile Tyr Leu His Asn Ser Trp Asn Lys Asn Tyr 20 25 30 Ile His Lys
Arg Glu Asn Phe His Asn Thr Ser Phe Ala Leu Ile Phe 35 40 45 Phe
Phe Ser Ser Ile Met Ser Ile Asn Tyr Gly 50 55 5539PRTArabidopsis
thaliana 55Met Phe Phe Phe Arg Leu Leu Phe Ile Ser Thr Ile Leu Gly
Thr Lys 1 5 10 15 Thr Thr Phe Thr Asn Glu Arg Ile Phe Thr Thr Pro
Leu Leu Leu Ser 20 25 30 Phe Phe Phe Phe Arg Pro Leu 35
566PRTArabidopsis thaliana 56Met Arg His Lys Glu Ser 1 5
5734PRTArabidopsis thaliana 57Met Asn Leu Leu Lys Lys Glu Arg Gln
Arg Arg Arg Gln Arg Ser Ile 1 5 10 15 Gly Ser His Cys Ile Ala Ser
Leu Val Leu Lys Asp Gly Tyr Met Lys 20 25 30 Lys Ile
589PRTArabidopsis thaliana 58Met Val Leu Ser Gly Lys Leu Thr Phe 1
5 5920PRTMedicago truncatula 59Met Leu Leu His Arg Leu Ser Lys Phe
Cys Lys Ile Glu Arg Asp Ile 1 5 10 15 Val Tyr Ile Ser 20
605PRTMedicago truncatula 60Met Lys Ile Glu Glu 1 5 6134PRTZea mays
61Met His Leu Pro Ser Thr Pro Ser Arg Pro Pro Pro Gln His Thr Ser 1
5 10 15 Leu Ser Phe Leu Gly Lys Glu Met Thr Lys Gly Thr Thr Thr Ala
Cys 20 25 30 Phe Gly 6210PRTArabidopsis thaliana 62Met Leu Ser Leu
Ser His Phe His Ile Cys 1 5 10 6323PRTMedicago truncatula 63Met Met
Val Phe Gly Lys Pro Lys Lys Ala Met Leu Val Arg Phe Asn 1 5 10 15
Pro Lys Thr Asp Leu His Val 20 6410PRTMedicago truncatula 64Met Ala
Ser Ala Ala Lys Val Tyr Met Ala 1 5 10 656PRTArabidopsis thaliana
65Met Ala Ser Lys Ile Trp 1 5 6610PRTArabidopsis thaliana 66Met Val
Arg Phe Gln Leu Ser Ile Arg Asp 1 5 10 6712PRTArabidopsis thaliana
67Met Cys Thr Tyr Tyr Tyr Leu Ile Asn Lys Tyr Phe 1 5 10
6811PRTArabidopsis thaliana 68Met Phe Pro Ala Lys Trp Cys Arg Leu
Glu Ser 1 5 10 6922PRTArabidopsis thaliana 69Met Gly Ser Leu Ser
Leu Phe Lys Ser Gln Leu Glu Ile Leu Met Leu 1 5 10 15 Leu Leu Ser
Leu Ser Lys 20 7020PRTArabidopsis thaliana 70Met Ser Val Tyr Ile
His Val Pro Ile Ser Leu Asn Cys Phe Ser Pro 1 5 10 15 Lys Ser Ser
Cys 20 7111PRTArabidopsis thaliana 71Met Gly Val Pro Asn Phe Arg
Pro Arg Asn Arg 1 5 10 7223PRTArabidopsis cebennensis 72Met Arg Ser
Arg Val Ser Phe Phe Phe Phe Lys Ile Met Leu Phe Arg 1 5 10 15 Leu
Leu Gly Tyr Arg Ser Met 20 7344PRTArabidopsis cebennensis 73Met His
Thr Tyr Ile His Thr Ile Ser Asn Ile Ser Ser Ile Phe Phe 1 5 10 15
Cys Ser Lys Arg Ser Phe Ser Pro Phe Thr Tyr Ile Arg Ile Ile Val 20
25 30 Val Ile Asp Pro Phe Arg Ile Ala Leu Thr Phe Arg 35 40
7422PRTArabidopsis halleri 74Met Arg Ser Arg Val Ser Leu Phe Leu
Ser Phe Ser Ser Asn Phe Ala 1 5 10 15 Ala Tyr Ser Pro Arg Ser 20
7548PRTArabidospis lyrata 75Met His Thr Tyr Ile Pro Ser Ser Ser Phe
Pro Ile Ser Asn Ile Ser 1 5 10 15 Ser Val Phe Phe Cys Tyr Lys Arg
Ser Phe Ser Pro Tyr Thr Tyr Ile 20 25 30 Arg Ile Ile Val Val Ile
Asp Pro Phe Arg Ile Ala Leu Thr Phe Arg 35 40 45 7650PRTArabidopsis
thaliana 76Met Asn Ile His Thr Tyr His His Leu Leu Phe Pro Ser Leu
Val Phe 1 5 10 15 His Gln Ser Ser Asp Val Pro Asn Ala Leu Ser Leu
His Ile His Thr 20 25 30 Tyr Glu Tyr Ile Ile Val Val Ile Asp Pro
Phe Arg Ile Thr Leu Ala 35 40 45 Phe Arg 50 7719PRTArabidopsis
thaliana 77Met Phe Gln Thr Leu Tyr Leu Phe Ile Tyr Ile His Thr Asn
Ile Leu 1 5 10 15 Leu Leu Ser 7845PRTBrassica rapa 78Met Phe Lys
Leu Tyr Phe Ser Ala Ile Leu Ser Thr Gln Tyr Met His 1 5 10 15 Thr
Tyr His His Arg Ile Ala Leu Ile Phe Leu Ser Ile Leu Tyr Pro 20 25
30 Ser Thr Asn Tyr Leu Met Ser Pro Ile Leu Asn Pro Thr 35 40 45
7920PRTCarica papaya 79Met Lys Ile Lys Leu Gly Phe Ser Leu Ile Lys
Ile Ile Ile Leu Leu 1 5 10 15 Asp Lys Asn Ser 20 8021PRTCapsella
rubella 80Met His Pro His Thr Tyr Ile His Ile Pro Ser Ser Ser Phe
Leu Ile 1 5 10 15 Ser Ser Phe Cys Leu 20 8145PRTEucalyptus grandis
81Met Lys His Ile Gln Arg Trp Arg Tyr Gly Glu Thr Ser Gly Arg Gln 1
5 10 15 Gly Asp Trp Lys Arg Leu Glu Ile Lys Val His Ser Asn Pro Ser
Leu 20 25 30 Lys Val Lys Lys Asn Thr Asn Asn Phe Ser Ser Ser Leu 35
40 45 8228PRTGossypium raimondii 82Met Ile His Phe Asn Leu Ser Gln
Trp Arg Ala Ile Cys Met Ala Asn 1 5 10 15 Phe His Leu Thr Tyr Ser
Phe Leu Phe Gly Val Leu 20 25 8321PRTMedicago truncatula 83Met His
Val Tyr Leu Glu Leu Phe Met Val Ile Lys Gly Leu Gly Phe 1 5 10 15
Leu Leu Leu Val Lys 20 8423PRTOryza sativa 84Met Glu Met Ile Gln
Arg Pro Cys Leu Ile Leu Lys Phe Phe Phe Lys 1 5 10 15 Leu Ser Thr
Leu Tyr Ile Pro 20 8538PRTPhyscomitrella patens 85Met Phe His Arg
Arg Arg Ser Ser Val Leu Leu Pro Pro Phe Gly Gln 1 5 10 15 Thr Gln
Pro Asn Pro Arg Cys Leu Pro Asp Leu Arg Phe Pro Ser Cys 20 25 30
Phe Thr Pro Cys Thr Ala 35 8642PRTThellungiella halophila 86Met Thr
Ile Cys Lys Val Ser Lys Ala Cys Phe Tyr Ala Gly Lys Ile 1 5 10 15
Glu Asn Ser Arg Leu Ile Lys Lys Ile Gly Ile Pro Lys Arg Glu Gly 20
25 30 Ala Pro Phe Ser Pro Ile Arg Glu Asn Gln 35 40
8737PRTThellungiella halophila 87Met Glu Ile Gln Ile Lys Lys Lys
Asn Leu
Tyr Ile Met Asn Thr Gln 1 5 10 15 Lys Leu Pro Asn Leu Tyr Ile Tyr
Ile Tyr Lys Tyr Val Phe Ile Lys 20 25 30 Leu Met Val Val Glu 35
8843PRTArabidopsis thaliana 88Met Val Pro Gln Ile Asn Leu Trp Ser
Ser Arg Val Ile Leu Lys Ile 1 5 10 15 Arg Ile Asp Ser Ser Thr His
Arg Glu Glu Asp His Cys Ile Gln Asn 20 25 30 His Lys His Gly Leu
Ser Phe Ile Phe Ser Phe 35 40 8916PRTArabidopsis thaliana 89Met Ser
Leu Gln Phe Tyr Glu Arg Val Ser Phe Lys Asn Thr Val Lys 1 5 10 15
9012PRTArabidopsis thaliana 90Met Thr Glu Gln Glu Glu Glu Ser Gln
Met Ser Thr 1 5 10 9139PRTArabidopsis thaliana 91Met Tyr Leu Gln
Tyr Ile Asp Asn Val Ile Ser Ile Tyr Ser Asn Asn 1 5 10 15 Arg Arg
Val Gly Arg Met Phe Ser Arg Val Pro Leu Ser Thr Ser Leu 20 25 30
Glu Ile Gln Phe Phe Ile Lys 35 9212PRTArabidopsis thaliana 92Met
Ser Lys Glu Ile Phe Phe Ser Pro Gly Phe Glu 1 5 10
9344PRTArabidopsis thaliana 93Met Arg Thr His Glu Gln Ser Thr Ala
Ile Thr Thr Leu Arg His Cys 1 5 10 15 Tyr Ser Ser Arg Phe Met Cys
Ser Gln Val Thr Pro Ala Glu Leu Phe 20 25 30 Leu Tyr Arg Pro Cys
Phe Ile Asn Ala Val Ala Arg 35 40 945PRTArabidopsis thaliana 94Met
Lys Arg Asn Met 1 5 955PRTArabidopsis thaliana 95Met Gln Cys Glu
Ile 1 5 964PRTArabidopsis thaliana 96Met Phe Cys Ala 1
976PRTArabidopsis thaliana 97Met Val Met Ala His His 1 5
9854PRTArabidopsis thaliana 98Met Met Lys Pro Arg Trp Asn Cys Ser
Leu Tyr Gly Ile Thr Glu Trp 1 5 10 15 Thr Asn Asn Gln Asn Gln Lys
Ser Lys Arg Lys Gly Arg Arg Lys Thr 20 25 30 Gln Ile Trp Arg Ile
Gly Asp Arg Leu Asp Thr Val Glu Cys Ile Thr 35 40 45 Leu Met Leu
Ser Ala Tyr 50 9910PRTArabidopsis thaliana 99Met Leu Leu Ile Ile
Val Glu Leu Val Leu 1 5 10 10014PRTArabidopsis thaliana 100Met Leu
Cys Phe Asn Phe Arg Cys Val Arg Arg Phe Ala Glu 1 5 10
10136PRTArabidopsis thaliana 101Met Tyr Thr Tyr Gln Leu Asp Asn Ser
Phe Ser Trp Phe Leu Cys Thr 1 5 10 15 Arg Phe Cys Leu Tyr Arg Tyr
Phe Leu Phe Asn Phe Arg Cys Phe Arg 20 25 30 Arg Phe Ser Glu 35
10288PRTDrosophila melanogaster 102Met Trp Arg Glu Val Cys Ala Gln
Lys Ser Gln Thr Lys Arg Arg Asn 1 5 10 15 Phe Ile Thr Gly Asn Gln
Arg Arg Asn Lys Thr Lys Ala Asn Arg Lys 20 25 30 Ala Glu Thr Lys
Gln Gln Lys Val Tyr Glu Phe Phe Val Gln Ala Arg 35 40 45 Glu Arg
Cys Lys Thr Arg Lys Lys His Glu Lys Lys Thr Leu Lys Lys 50 55 60
Thr Lys Lys Ile Gln Asn Arg Tyr Arg Ala Val Ser Glu Asn Glu Trp 65
70 75 80 Gly Lys Gly Phe Pro Ser His Ile 85 10336PRTDrosophila
melanogaster 103Met Arg Thr Lys Lys Ser Asn Lys Lys Ala Gln Phe Tyr
Tyr Gly Gln 1 5 10 15 Pro Thr Thr Lys Gln Asn Lys Ser Gln Pro Lys
Ser Arg Asn Lys Ala 20 25 30 Ala Lys Ser Leu 35 10471PRTDrosophila
melanogaster 104Met Glu Pro Gly Phe Val Phe Val Leu Phe Pro Thr His
Leu Ser Thr 1 5 10 15 Gln His Thr Gln Arg Glu Lys Ser Ile Leu Val
Met Gly Leu Asn Leu 20 25 30 Gln Ser Ala Lys Gln Ser Asp Lys Gln
Asn Ser Lys Glu Arg Lys Lys 35 40 45 Asn Thr Gln Ile Asn Ser Gln
Arg Ile Pro Tyr Arg Gln Gly Gly Gln 50 55 60 Cys Ser Lys Val Leu
Ser Pro 65 70 10592DNAArabidopsis thaliana 105atgttctgtt caattcaatg
cgtcgccaga catctgttcc ctttgcatgt aagagagata 60aagaaagcga caagagccat
aaagaaaggt aa 9210675DNAArabidopsis thaliana 106atgcgtcgcc
agacatctgt tccctttgca tgtaagagag ataaagaaag cgacaagagc 60cataaagaaa
ggtaa 75107114DNAArabidopsis thaliana 107atggttatgt tttttctcga
tttagacaaa aaccctagat ttgatcttct aaagggtctc 60aaatggaatc tcttctcttc
tcatatctct ccctctctcc ctccctctct ttga 11410836DNAArabidopsis
thaliana 108atgaaggaca actttcctct tctccttcgg ttataa
3610915DNAArabidopsis thaliana 109atgagtgatg actga
1511051DNAArabidopsis thaliana 110atgatatata taaataaata tgggtcgata
tcggctgtgg aggacgacta g 5111115DNAArabidopsis thaliana
111atgagccaaa gataa 1511251DNAArabidopsis lyrata 112atgacgtgtc
ctcttctctc tctctctttc cttctctcta agtatattta g 5111351DNAArabidopsis
thaliana 113atgacgtggc ctcttctctc tctctctttc cttctctcta agtatgttta
g 5111453DNACapsella rubella 114atgacgtgta ctctctctgc tctatctctc
tctctaaata tgtttagggt taa 5311518DNAArabidopsis thaliana
115atgttttatc tttcataa 18116114DNAArabidopsis thaliana
116atggttaata ctagtagctt tttcatttca agttttatcc ttccattggt
tctttctgag 60tcaaattgtc tcctgtttcg aaccatatat aagttttcaa tggttttgta
ttaa 11411775DNAArabidopsis thaliana 117atgttttgtt tgttgattcc
catcttctct tttgtctttt caccaaatcg tcatttaagg 60cttcaagaac agtaa
7511818DNAArabidopsis thaliana 118atgttttccc ctcaatga
1811972DNAArabidopsis thaliana 119atgaaataca tacacatttt gattttattt
aaatcaagat cgacgtataa gctatccacc 60aatcatattt aa
7212033DNAArabidopsis thaliana 120atgaaaattc cattgtttct gccgaagctt
tga 3312184DNAArabidopsis thaliana 121atggtatctg gtcaagaaga
ttcctggtta aaactttcat ctctctgttt cctttttctt 60tctttgttgg attcattaat
ttga 84122105DNAArabidopsis thaliana 122atgtttcttt taatcttttt
gagattaata atgatttgtg tttgttcatc aaccgatttt 60ctcagatctg tcaattattt
ttgtttattt atttatgatt tatga 10512330DNAArabidopsis thaliana
123atgtccacta ctcaagagca taggtcttga 3012478DNAArabidopsis thaliana
124atgatactaa agtgctggag ttcccggttc ctgagagtga gtccatatca
aaatgcgcat 60tcgttatcac ttggttga 7812542DNAArabidopsis lyrata
125atgcccttag cagttattag acaagggatt gtttggccct ag
4212675DNAArabidopsis lyrata 126atgccatcat ggcatgacat ggttcttttg
ccttacgtaa aacacactca cgccaacaca 60cgccacataa cataa
7512727DNAArabidopsis lyrata 127atgacatggt tcttttgcct tacgtaa
27128114DNAArabidopsis thaliana 128atgccatcat ggcatggtat ggttcttttg
ccttacgtaa aacacactca cgccagcaca 60cacacacaca cacataacat atacggatgt
gcgtgtgagc tagtcttcca ttaa 11412969DNAArabidopsis thaliana
129atggcatggt atggttcttt tgccttacgt aaaacacact cacgccagca
cacacacaca 60cacacataa 6913027DNAArabidopsis thaliana 130atggtatggt
tcttttgcct tacgtaa 2713124DNABrassica rapa 131atgatgataa ttttgtggaa
ataa 2413275DNABrassica rapa 132atgctttggg ccaagctagt ttcttttagc
actcttcact cactagtttt tcttctcagc 60ccttcttttg cgtga
75133120DNABrassica rapa 133atgccatcat ggcatggcat tgtcattttg
cctttcgtaa aacacactca cgccaacata 60cattattcat attcatgtgt atgtatatga
atgccatcat ggcatatgcc atcatggcat 120134105DNACarica papaya
134atgattgcat gccatcccta cttgcctttt ccccttttcc tttctctaac
attttactca 60atcttcttct ccccctcccc cccttccccc tctctgccat tataa
105135162DNACarica papaya 135atgccatccc tacttgcctt ttcccctttt
cctttctcta acattttact caatcttctt 60ctccccctcc cccccttccc cctctctgcc
attataacca taattaaacc tctctccctc 120tctctccctc tctctctctc
tctctctggg ttctcagtat aa 16213648DNACapsella rubella 136atggaattaa
aaggtttgag aacttggcag ttattagaca aggtatag 4813745DNACapsella
rubella 137atgccatcat ggcatggcat ggcatgtttc tattgcctta cgtaa
4513890DNACapsella rubella 138atggcatggc atgtttctat tgccttacgt
aaaacacact cacgccaaca catactcact 60atacatgtaa ataagtatgt gcgcgtgtga
90139108DNAGossypium raimondii 139atgatgagat caagaatttt aaagtttcaa
tatagatttg gcatgggtat tggcggcaga 60aagcaattaa aaaaccagtt atgtcaaatt
caaggtcgta tcagttaa 10814033DNAGossypium raimondii 140atgtcaaatt
caaggtcgta tcagttaaaa tga 3314154DNAGossypium raimondii
141atgaatgaag atttagaaat ttcaacaagg aagaggaccc cacagctttg ttaa
5414239DNAMedicago truncatula 142atgcccaaat ttgatatttt tttttatata
tttgtatag 39143231DNAMedicago truncatula 143atgtcatata tctctctctc
tcctaagttg ctacctataa atactaagcc tttcccttgg 60ttggttcaat tcaacttcta
cttctcatca aacacaaagt gcaataagct tcatttcctg 120ggtgagaagc
tccttgttgg agaagcaggg cacgtgcaaa tcctctttct gattcattct
180ctcataatgc atatcaatat cttttgcacg tgctcccctt ctccaactag g
23114463DNAOryza sativa 144atgcaaaccc actccaacac tccacaatcc
acatactctc tctctctctc tctctctgag 60tag 6314548DNAOryza sativa
145atgtgtgtgt gtgatatcaa tatgcattcg atgttgatgc tactgtag
4814678DNAArabidopsis lyrata 146atgagaatta agctatttca gttgagggga
atgttgtctg gatcgaggat attatacata 60tatacatgtg tatgttga
7814757DNAArabidopsis thaliana 147atgagggtta agctatttca gttgagggga
atgttgtctg gatcgaggat attatag 57148111DNABrassica carinata
148atgagaatga agctatttca gttgagggga atgttgtctg gatcgaggat
attatatata 60cacaaatacg tatatatgtt aatacaagtg tttgatcata tatgtatata
g 11114984DNABrassica juncea 149atgagaatga agctatttca gttgagggga
atgttgtctg gatcgaggat attatatata 60cacaaatatg tatatatatg ttaa
84150111DNABrassica napus 150atgagaatga agctatttca gttgagggga
atgttgtctg gatcgaggat attatatata 60cacaaatacg tatatatgat aatacaagtg
tttgatcata tatgtatata g 111151111DNABrassica oleracea 151atgagaatga
agctatttca gttgagggga atgttgtctg gatcgaggat attatatata 60cacaagtacg
tatatatgtt aatacaagtg tttgatcata tatgtatata g 11115284DNABrassica
rapa 152atgagaatga agctatttca gttgagggga atgttgtctg gatcgaggat
attatatata 60cacaaatatg tatatatatg ttaa 8415360DNAArabidopsis
thaliana 153atgttggatc tctttcgatc taacaatcga attgaacctt cagatttcag
atttgattag 6015415DNAArabidopsis thaliana 154atgagagata gataa
1515533DNAArabidopsis thaliana 155atgaacagaa aaatctctct ttctctttct
tga 3315627DNAArabidopsis thaliana 156atgatgggtt gttttgtggg attttaa
2715727DNAArabidopsis thaliana 157atgcaggagg aaacatatga ggggtga
27158180DNAArabidopsis thaliana 158atgccacata caaacttgaa agatctcttc
atcttttctc caaatgtttt tttttcgttt 60gctatttatc tccacaattc ttggaacaaa
aactacattc acaaacgaga gaattttcac 120aacacctctt ttgctctcat
tttttttttt tcgtccatta tgagtattaa ttatggttag 180159120DNAArabidopsis
thaliana 159atgttttttt ttcgtttgct atttatctcc acaattcttg gaacaaaaac
tacattcaca 60aacgagagaa ttttcacaac acctcttttg ctctcatttt ttttttttcg
tccattatga 12016021DNAArabidopsis thaliana 160atgagacata aagagagtta
a 21161105DNAArabidopsis thaliana 161atgaacctcc tcaagaagga
aagacagagg aggagacaaa gaagtatagg ttcacattgc 60atagccagtt tagttttgaa
ggatggatat atgaaaaaaa tatga 10516230DNAArabidopsis thaliana
162atggttctct ccggtaaatt aacattttag 3016363DNAMedicago truncatula
163atgcttcttc ataggctctc caaattttgc aaaattgaaa gagacatagt
atatatatct 60tag 6316418DNAMedicago truncatula 164atgaagattg
aagagtaa 18165105DNAZea mays 165atgcatctgc cttcaactcc ctctcgcccc
ccaccccaac acacatctct ctcttttcta 60gggaaggaaa tgacgaaggg gacgacgacg
gcatgcttcg gctag 10516633DNAArabidopsis thaliana 166atgttgtctc
tttctcattt tcatatctgc taa 3316772DNAMedicago truncatula
167atgatggtgt ttgggaagcc gaaaaaagcg atgttggtga ggttcaatcc
gaagacggat 60ttacatgtat ag 7216833DNAMedicago truncatula
168atggcttcag ctgcaaaagt atacatggcg tga 3316921DNAArabidopsis
thaliana 169atggcttcca agatctggta a 2117033DNAArabidopsis thaliana
170atggttaggt tccaactaag tatacgagat taa 3317139DNAArabidopsis
thaliana 171atgtgtacgt actattatct cataaataaa tatttttaa
3917236DNAArabidopsis thaliana 172atgtttccag caaaatggtg ccgtcttgag
tcttga 3617369DNAArabidopsis thaliana 173atgggatctc tctctttatt
taaaagtcaa ttagagatct tgatgctact tctgtccctt 60tccaagtga
6917463DNAArabidopsis thaliana 174atgagtgtat atattcatgt acctatctct
ctcaattgct tctcaccaaa atcatcttgc 60tga 6317536DNAArabidopsis
thaliana 175atgggagttc ccaactttag acctcgaaac cgataa
3617672DNAArabidopsis cebennensis 176atgagatcta gggtttcttt
ctttttcttc aaaatcatgc tttttcgctt gctaggttat 60agatccatgt aa
72177134DNAArabidopsis cebennensis 177atgcatacat acatacatac
catctctaat atttcatcaa tcttcttttg ttccaaacgc 60ctttctctcc atttacatac
atacgaatca ttgttgtcat agatccgttt agaattgctt 120taacttttag atga
13417869DNAArabidopsis halleri 178atgagatcta gggtttcttt gtttctttcg
ttttcttcaa attttgctgc atattctcca 60agatcatga 69179147DNAArabidospis
lyrata 179atgcatacat acataccatc atcatctttt cccatctcta atatttcatc
agtcttcttt 60tgttacaaac gctctttctc gccatataca tacataagaa tcattgttgt
catagatccg 120tttagaattg ctttaacttt tagatga 147180153DNAArabidopsis
thaliana 180atgaatatac atacatacca tcatcttctt ttcccatctc tagtttttca
tcaatcttct 60gatgttccaa acgctctatc tcttcatata catacatacg aatatattat
tgttgtcata 120gatccattta gaatcacttt agcttttaga tga
15318160DNAArabidopsis thaliana 181atgttccaaa cgctctatct cttcatatac
atacatacga atatattatt gttgtcatag 60182138DNABrassica rapa
182atgtttaagc tctacttctc agcaattctc tccacccaat acatgcatac
ataccatcat 60cgtatcgctc taatttttct atcaatcttg tatccttcca caaattatct
tatgtctccc 120attttaaatc ctacatag 13818363DNACarica papaya
183atgaagatta aattaggttt tagtcttatt aagattatta tattactaga
caaaaacagt 60taa 6318466DNACapsella rubella 184atgcatccac
atacatacat acatatacca tcatcttctt ttctcatctc tagtttttgt 60ttataa
66185138DNAEucalyptus grandis 185atgaagcata ttcaaaggtg gagatatggg
gagacttccg gaaggcaagg ggattggaaa 60aggctcgaga tcaaagtgca tagcaaccct
tcgctaaagg tgaaaaagaa tacgaataac 120ttcagtagct cactttaa
13818687DNAGossypium raimondii 186atgatccatt tcaacctgtc acagtggaga
gcaatttgta tggctaattt ccatctcacc 60tattcttttc tgtttggggt tctctag
8718766DNAMedicago truncatula 187atgcatgtat atcttgaatt gtttatggta
ataaaggggt taggatttct ccttttggtg 60aagtga 6618872DNAOryza sativa
188atggaaatga tacaaaggcc gtgtttaatt ttaaaatttt ttttcaaact
ttcaacactt 60tacatcccat aa 72189117DNAPhyscomitrella patens
189atgttccacc gtcggagatc ctcggtgctg ctacccccgt tcggccaaac
ccaacccaac 60cctaggtgtc tgccggacct ccgcttcccc tcctgcttca ccccctgcac
cgcttaa 117190129DNAThellungiella halophila 190atgacgatat
gtaaagtaag caaggcatgt ttttatgcag ggaagattga aaattcaaga 60ttaatcaaga
aaattggaat accaaaaaga gagggagctc ccttcagtcc aatcagagag
120aatcaatga
129191114DNAThellungiella halophila 191atggagattc aaattaaaaa
gaaaaactta tatataatga atacacaaaa gctacctaat 60ctgtatatat atatatataa
atatgtcttc attaaattaa tggtcgtgga atag 114192132DNAArabidopsis
thaliana 192atggtacctc aaattaatct atggtcatct agggttatct tgaagattag
aattgattct 60agcacgcaca gagaggaaga tcattgcatc cagaatcaca aacatggcct
atcttttatc 120ttttcttttt ga 13219351DNAArabidopsis thaliana
193atgtctctcc aattttatga gagggtttcc ttcaagaaca cagtaaaata g
5119439DNAArabidopsis thaliana 194atgacagagc aagaagaaga aagtcaaatg
tccacatga 39195120DNAArabidopsis thaliana 195atgtatctac aatatattga
taatgtaata tctatatatt caaacaatcg tcgtgttggt 60cggatgtttt ctagagttcc
tctgagcact tcattggaga tacaattttt tataaaatag 12019639DNAArabidopsis
thaliana 196atgagcaagg agatattttt ttcccctggg tttgaatga
39197133DNAArabidopsis thaliana 197atgagaacac acgagcaatc aacggctata
acgacgctac gtcattgtta cagctctcgt 60ttcatgtgtt ctcaggtcac ccctgctgag
ctctttctct accgtccatg ttttatcaac 120gccgtggccc gtg
13319818DNAArabidopsis thaliana 198atgaagagaa acatgtaa
1819918DNAArabidopsis thaliana 199atgcaatgtg aaatatga
1820015DNAArabidopsis thaliana 200atgttttgtg cttga
1520121DNAArabidopsis thaliana 201atggtcatgg ctcatcatta g
21202165DNAArabidopsis thaliana 202atgatgaaac ctcgatggaa ctgctctctt
tatggaatca cggaatggac aaataatcaa 60aatcagaaat cgaagcgaaa agggaggaga
aaaacgcaga tttggaggat tggggacaga 120ttagatactg ttgaatgcat
cactctaatg ctatcagcct attaa 16520333DNAArabidopsis thaliana
203atgctgctta tcatcgtgga gttggttctg taa 3320445DNAArabidopsis
thaliana 204atgctttgtt tcaatttcag gtgcgttaga aggtttgcag agtag
45205108DNAArabidopsis thaliana 205atgtacacct accagcttga taactctttt
tcgtggtttc tgtgtactcg tttctgtttg 60tacagatact tcttgttcaa tttcagatgc
tttagaaggt tttcggag 108206267DNADrosophila melanogaster
206atgtggcgcg aagtatgcgc acaaaaaagt caaacaaaaa ggcgcaattt
tattacgggc 60aaccaacgac gaaacaaaac aaaagccaac cgaaaagcag aaacaaagca
gcaaaaagtt 120tatgaatttt ttgtgcaggc gcgtgaaaga tgcaaaacga
gaaaaaaaca tgaaaaaaaa 180acattaaaaa aaacaaaaaa aatccaaaac
agataccgag ctgtatccga aaacgagtgg 240ggaaaggggt ttcccagtca catataa
267207111DNADrosophila melanogaster 207atgcgcacaa aaaagtcaaa
caaaaaggcg caattttatt acgggcaacc aacgacgaaa 60caaaacaaaa gccaaccgaa
aagcagaaac aaagcagcaa aaagtttatg a 111208216DNADrosophila
melanogaster 208atggagcctg gctttgtttt tgttttattt ccaacccact
tgagcacaca gcacacacag 60agagaaaaat caatactcgt tatgggatta aatttacaaa
gcgcaaagca aagcgacaaa 120caaaattcaa aagaaagaaa aaaaaacact
caaataaact cacaaagaat tccttatcgc 180caagggggcc aatgttctaa
ggttctttcg ccttga 216209614DNAArabidopsis thaliana 209attcattgtt
cactctcaaa tctcaagttc attgccattt ttaggtctct ctataaattc 60aaatgttctg
ttcaattcaa tgcgtcgcca gacatctgtt ccctttgcat gtaagagaga
120taaagaaagc gacaagagcc ataaagaaag gtaagactct ttgaaataga
gagagataag 180gttttctctt atcttcttct catcagatct ttgtttcttt
accctctttc tttctttttt 240ttgcttttta tggttatgtt ttttctcgat
ttagacaaaa accctagatt tgatcttcta 300aagggtctca aatggaatct
cttctcttct catatctctc cctctctccc tccctctctt 360tgattctttg
tcttctccag ttaaaactca gatctaacac aaagcttaaa agattctcat
420cgtttcttgt tttctttgtt tcatcttgta gatctctgaa gttggactaa
ttgtgaatga 480aagagttggg acaagagaaa cgcaaagaaa ctgacagaag
agagtgagca cacaaaggca 540atttgcatat cattgcactt gcttctcttg
cgtgctcact gctctttctg tcagattccg 600gtgctgatct cttt
614210422DNAArabidopsis thaliana 210ctctgccttt agttctttct
tttttggtaa tatatttatt tttcgttacg atttggtcaa 60aaccctagat ttgttttcca
aaagcatatc tgaaaatgaa ggacaacttt cctcttctcc 120ttcggttata
aatattctct ccggttttgc ttgtttaacc taaaagcctc agatctaact
180ccaacacctt caaagtctgc ctcctttcca atcttctttc ttctgttcga
tctctaatct 240cagaatttgt gtcggtaagg taaaggtgat aatgagtgat
gactgatgag ggagttttgg 300gacaaatttt aagagaaacg catagaaact
gacagaagag agtgagcaca caaaggcact 360ttgcatgttc gatgcatttg
cttctcttgc gtgctcactg ctctatctgt cagattccgg 420ct
422211268DNAArabidopsis thaliana 211tcccacatcc aaagatagaa
agatgtaagg tctagagtct tgttcttaat cccctaacag 60aacaatgata tatataaata
aatatgggtc gatatcggct gtggaggacg actagctacg 120gtttcgagcc
tggtcacatg cgtagagtgt gaaaggtaat taggaggtga cagaagagag
180tgagcacaca tggtggtttc ttgcatgctt ttttgattag ggtttcatgc
ttgaagctat 240gtgtgcttac tctctctctg tcacccct
268212328DNAArabidopsis thaliana 212tcccacagcc aatgagccaa
agataaagaa acacctatcc tataataatt tagagcaata 60tacctccata atggaacatc
tatatatata aaggtatccg tatatctcta tatattatat 120tcattgagtt
taaagtggct agggtttata gatgtatgtg atattaagag atatgaaaca
180tatttgtcga cggtttgagt ggtgaggaat tgatggtgac agaagagagt
gagcacacat 240ggtggctttc ttgcatattt gaaggttcca tgcttgaagc
tatgtgtgct cactctctat 300ccgtcacccc cttctctccc tctccctc
328213592DNAArabidopsis lyrata 213aaaaaatgac gtgtcctctt ctctctctct
ctttccttct ctctaagtat atttagggtt 60aattattagg gttctttatc tctttcttca
gtctttgaag tttcttcaat agctttaatt 120gaagtgattt acctctctgg
gtgtttttag tatatatatc atgtacatga tcgaatttct 180ttctatccaa
gttctcatca aaccttctca tgttttgaag agttaaaggc tttatagttt
240gcttaggtca gatccataac atactgtatt tgacaagttt ctttgtctca
cgatagatct 300tggtctgacc aaaatgattt tctcgagaaa aaaaaagatg
gaagtagagc tccttgaagt 360tcaaacgaga gttgagcagg gtaaagaaaa
gctgctaagc tatggatccc ataagcccta 420atccttataa agaaaaaaaa
ggatttggtt atatggcttg catatctcag gagctttaac 480ttgcccttta
atggctttta ctcttctttg gattgaaggg agctctacat cttctttcac
540cttctctatt tttctttctt tattttctcc tctacagtaa tttatttgga tt
592214599DNAArabidopsis thaliana 214ttccaaaaca tgacgtggcc
tcttctctct ctctctttcc ttctctctaa gtatgtttag 60ggttaataat tagggttcct
cctctctttt gttctgtctt tatatctcct tcatagctct 120aatgtaagag
atttacctct tttggtgttt ttgttaatcc acgttctcat caaaactttc
180tcattgtttt atgaagagtt aaaggtcttt acagtttgct tatgtcagat
ccataatata 240tttgacaaga tactttgttt ttcgatagat cttgatctga
cgatggaagt agagctcctt 300aaagttcaaa catgagttga gcagggtaaa
gaaaagctgc taagctatgg atcccataag 360ccctaatcct tgtaaagtaa
aaaaggattt ggttatatgg attgcatatc tcaggagctt 420taacttgccc
tttaatggct tttactcttc tttggattga agggagctct acatcttctt
480tcaccttctc tattttttat ttttctttat ttctactcaa caattattta
ttcggattca 540tctttaattt tccgttataa tttctttttg gtaaggatta
ttcgctataa tttgagaat 599215623DNACapsella rubella 215ttccaacgaa
tgacgtgtac tctctctgct ctatctctct ctctaaatat gtttagggtt 60aattagggtt
cttcatctgt ctctctctct ctctctcttc agagtcttta tagcttcttc
120caagattttt aattgaaagt aatttacctc ttttggagtt ctgtacatat
agaatatcag 180gagtcgtgtt tcttttttat caaggttctc atctaacctt
tatagtattt tcattagttg 240ataaaggtct tcatagtttg cttagatcag
atcttgtctt cgtcttttcg atagatcttg 300ttctgtccaa tatacagtga
ttttatttcg agagcaaaaa agatgagagg tagagctcct 360tgaagttcaa
acgagagttt agcagggtag agaaaagctg ctaagctatg gatcccataa
420gccctaatcc ttgttaatga taaaggattt ggttatatgg cttgcatatc
tcaggagctt 480taacttgccc tttaattgct tttactcttc tttggattga
agggagctct acatcttctt 540tgacttctct ctctattaag tctttcttta
ttttcttctc tacaatagtt gttttggatc 600ggaagatctt taagtttccc tta
623216612DNAArabidopsis thaliana 216tttcactttt gttctcctcc
tccctttttt tcttttcagg attcttcttt tctatgtttt 60atctttcata atagatctga
taattttgat ttttcactat atatattatg gttaatacta 120gtagcttttt
catttcaagt tttatccttc cattggttct ttctgagtca aattgtctcc
180tgtttcgaac catatataag ttttcaatgg ttttgtatta actcaagtat
tcaacattat 240gtctctcttt ttcttgcttg gatctctaat gctgttcata
ttttaaagca taggtttagg 300ttagatgcat gtaactgcca attaaaagaa
ggtcaagagt tttttgattg tatgaatata 360tgagttagtc aaagcagatc
cacacgatta tatagaaaaa caaaggaaga agaagaggaa 420gagctccttg
aagttcaatg gagggtttag cagggtgaag taaagctgct aagctatgga
480tcccataagc cttatcaaat tcaatataat tgatgataag gtttttttta
tggatgccat 540atctcaggag ctttcactta cccctttaat ggcttcactc
ttctttggat tgaagggagc 600tcttcatctc tc 612217515DNAArabidopsis
thaliana 217catcccaccc ttaattgttt tatataaacc atttctcctc ctctctccat
caccttcaat 60ctctctcgat ctctctctgg atccccaatc tcacctccat gttttgtttg
ttgattccca 120tcttctcttt tgtcttttca ccaaatcgtc atttaaggct
tcaagaacag taaccccaat 180tcctccacaa gagggagaga aaacaaaaga
tcttccaatt ccattctcgt acatgcaaat 240cacaatccat gccatagatt
gtttctattc ctccttattt attgcttgta tctgttcatg 300catggaccag
gtggagagag cattacttaa aaatagaatt agctatctgt tttaggcgaa
360ttagtttcct tacataacca tgtatatgtc atgacgcata tacatatgta
gatgtatatg 420tattatatat gtatgcctgg ctccctgtat gccatatgct
gagcccatcg agtatcgatg 480acctccgtgg atggcgtatg aggagccatg catat
515218276DNAArabidopsis thaliana 218actcataact ctccccaaat
tcttgaccaa aaatatccgc cactttctct ctggttcatg 60ttttcccctc aatgaaatac
atacacattt tgattttatt taaatcaaga tcgacgtata 120agctatccac
caatcatatt taagggttcc cgtatacata tatactatat atatatatgg
180aataatagtc gtgcctggct ccctgtatgc cacaagaaaa catcgattta
gtttcaaaat 240cgatcactag tggcgtacag agtagtcaag catgac
276219243DNAArabidopsis thaliana 219ctctaactca tccttctctt
ctatgaaaat tccattgttt ctgccgaagc tttgatcagt 60acttctcttt tgcttgatct
cggtttttga ccagtttatt gcgtcgatca atgcattgaa 120agtgactaca
tcggggttcc gatttttttt gttcttcata tgatgaagcg gaaacagtaa
180tcaaccctgg tttagtcact ttcactgcat taatcaatgc atttgtaaaa
agagggaaaa 240gca 243220537DNAArabidopsis thaliana 220ctagaagaaa
aaaccagatc tataaagttt gttattaaaa gatagagaga gaggagggat 60gtagtaggcc
aataggcaaa tcagagaatc acaaatggta tctggtcaag aagattcctg
120gttaaaactt tcatctctct gtttcctttt tctttctttg ttggattcat
taatttgaca 180tatctctatc atcacactga ttctctttct cccagtttgt
ctgcagatgc atgtgtgtaa 240tctagggtat atgtttttgt ccatttggtt
tcataaggca ataaagatcc agctatttac 300tacttgtggt atagattttg
actgttgaat tttcagatct gatgtgtttc gtttgatccg 360attcggaaaa
tttatgtttc gttgacattt tggagtttag ttggaagaag agtgagagtc
420gctggaggca gcggttcatc gatctcttcc tgtgaacaca ttaaaaatgt
aaaagcatga 480atagatcgat aaacctctgc atccagcgtt tgcctcttgt
atctttctta ttgactt 537221261DNAArabidopsis thaliana 221ctgcatctat
ccacctctct ctgtaaattt atctaaatgt ttcttttaat ctttttgaga 60ttaataatga
tttgtgtttg ttcatcaacc gattttctca gatctgtcaa ttatttttgt
120ttatttattt atgatttatg aatgaggaaa gagtgaagtc gctggaggca
gcggttcatc 180gatcaattcc tgtgaatatt tatttttgtt tacaaaagca
agaatcgatc gataaacctc 240tgcatccagc gctgcttgct c
261222481DNAArabidopsis thaliana 222tatcacagtt ctcatcaaat
atttgaaagt atcaaacaaa aaaaggagag tgagaaaaat 60aaagagagag atagagagag
atcatgtcca ctactcaaga gcataggtct tgattggtgg 120aagacaagta
ccttagataa accgaccaaa acccggtgga taaaatcgag ttccaacctc
180ttcaacgaca acgatttcaa cactctcttc caggaacaac ttcctccagg
cagatgatac 240taaagtgctg gagttcccgg ttcctgagag tgagtccata
tcaaaatgcg cattcgttat 300cacttggttg aacccatttg gggatttaaa
tttggaggtg aaatggaacg cgtaattgat 360gactcctacg tggaacctct
tcttaggaag agcacggtcg aagaagtaac tgcgcagtgc 420ttaaatcgta
gatgctaaag tcgttgaaga ggacttggaa cttcgatatt atcccccgtg 480t
481223471DNAArabidopsis lyrata 223agtagggttg gaaaattttt ttacattttt
actctaaaat agaatagagt tggagatgcc 60cttagcagtt attagacaag ggattgtttg
gccctagcga tcctctcttc actctctcac 120ttttgtagtt caacccttct
tttgcgtgag atgccatcat ggcatgacat ggttcttttg 180ccttacgtaa
aacacactca cgccaacaca cgccacataa cataaataaa ttatatatac
240atatacgtat gtgcgtgtga gtcttccatt aatgcaatct ttgggcctat
atatatatac 300aaaccttcca taaccaaagt tatcatacta caaaagctct
ctcgtacttg gaaatgcggg 360tgagaatctc catgttggag aagcagggca
cgtgcaaacc aacaaacacg aaatccgtct 420catgtgtttt gcacgtactc
cccttctcca acatgagctc ctgacccatt g 471224466DNAArabidopsis thaliana
224agacaagccc ccacactaaa aaaacagtaa tatggaataa aaaaaagctt
tcaaaactta 60gcagttatta gacaaggtat tgtttggccc tagctagcga tcgtttagct
ctcttcactc 120tctcactttt ttagttcaac ccttcttttg cgtgagatgc
catcatggca tggtatggtt 180cttttgcctt acgtaaaaca cactcacgcc
agcacacaca cacacacaca taacatatac 240ggatgtgcgt gtgagctagt
cttccattaa tgcaatcttt gggcctatat atacaaacct 300ttccataacc
aaagttctca tactacaaac gcccctcatg tgcttggaaa tgcgggtgag
360aatctccatg ttggagaagc agggcacgtg caaaccaaca aacacgaaat
ccgtctcatt 420tgcttatttg cacgtactta acttctccaa catgagctct tcaccc
466225450DNABrassica rapa 225agacaacccc acgttttaaa ataagaaatg
atgataattt tgtggaaata aaagctagta 60tacttttgca ataattagac aaggtattga
tgctttgggc caagctagtt tcttttagca 120ctcttcactc actagttttt
cttctcagcc cttcttttgc gtgaaatgcc atcatggcat 180ggcattgtca
ttttgccttt cgtaaaacac actcacgcca acatacatta ttcatattca
240tgtgtatgta tatgaatgtt ccattaatgc aatctttggg gcctatatat
acgaagctta 300catcaccaaa gctctcatat tacaaaagct cacatatata
cttggaaatg taggtgagaa 360cctccatgtt ggagaagcag ggcacgtgca
aaccaaaaaa catgaaatct gtttcatatg 420ctttgcacgt gctcccctcc
tccaacatga 450226513DNACarica papaya 226agacaacact cctctttgtt
cccttcctca cgtatccact tttgaaattt gtaatttgtg 60tgcaccacca tgattgcatg
ccatccctac ttgccttttc cccttttcct ttctctaaca 120ttttactcaa
tcttcttctc cccctccccc ccttccccct ctctgccatt ataaccataa
180ttaaacctct ctccctctct ctccctctct ctctctctct ctctgggttc
tcagtataaa 240tgcagctctg cttatacttc cacacctata tatatatacc
tgacccttct tcacctcctt 300catccacctc ctccttcttc cccaaaactt
tcttaactgt tctctgcata catatatatc 360cacatacata tatatatata
tagagagaga gtgagacaga gaggttaccg aggcaattgg 420gtgagtagct
ccctgttgga gaagcagggc acgtgcaaat tctccatggc tttcccctct
480ttgcacgtgc tccccttctc caacatgggt tcc 513227464DNACapsella
rubella 227cggccacccc cacatttaac aagaaaaaaa ctgatggaat taaaaggttt
gagaacttgg 60cagttattag acaaggtata gtttggccct agcttctttt aatttagctc
tctccactct 120cacacttttc aactttcacc cttctcttgc gtgagtcgcg
agatgccatc atggcatggc 180atggcatgtt tctattgcct tacgtaaaac
acactcacgc caacacatac tcactataca 240tgtaaataag tatgtgcgcg
tgtgagtctt ccatccatca atgcaatctt tggggctata 300tatatacaaa
ccttttccat aaccaaagct ctcatataaa ctacaaaagg ctcacttggg
360aaatgcgggt gagaatctcc acgttggaga agcagggcac gtgcaaacca
acaaacacga 420aaccctcctc atgtgctttg cacgtactcc ccttctccaa catg
464228571DNAGossypium raimondii 228gaaaacccaa gttcaggcta acaagttatc
tgatgatgag atcaagaatt ttaaagtttc 60aatatagatt tggcatgggt attggcggca
gaaagcaatt aaaaaaccag ttatgtcaaa 120ttcaaggtcg tatcagttaa
aatgaatgaa gatttagaaa tttcaacaag gaagaggacc 180ccacagcttt
gttaaattaa gtgtgtggtt tttataatta tcatctcgaa agtttcataa
240tatcaattag attaaaacat ctctgaattt cataattaca aaccagatag
atagatacat 300gaaaacttag accccagaga tctgtcttta aagaatgccc
acttctagac tcaatctcta 360ttactctctt tttttctctc tctctctctt
cggaaaaact tgtatataaa taaatgacac 420tttctttgct ttctgcactc
aactcatgaa cttgaaaagc tttacttgga tgggttggtt 480gggggtgagt
atctcttgtt ggagaagcag ggcacgtgca agttcctatg tttaagtgaa
540ctttgcacgt gctccccttc tccaccgtga g 571229592DNAMedicago
truncatula 229gaagagaaaa aacctagtgt aaaatttgat atactcttta
tgtatagtac gaatgttttt 60ttaaaaatta tgtaaaaaat gataaaataa taactaacta
aattaacagt aaaattagaa 120aagtaaaata ctatgcccaa atttgatatt
tttttttata tatttgtata gattattatt 180atttgatatg taaagtccaa
ttaaaaattt gttttaacta agatttgaac taggttttct 240taaaagactc
atcttttact tcaaatttat ttatcatttg aattcaatca ctttctaata
300ttattattat tatttccacc atactcattg cttctgccac gttactttag
ttagatctct 360tatgtcatat atctctctct ctcctaagtt gctacctata
aatactaagc ctttcccttg 420gttggttcaa ttcaacttct acttctcatc
aaacacaaag tgcaataagc ttcatttcct 480gggtgagaag ctccttgttg
gagaagcagg gcacgtgcaa atcctctttc tgattcattc 540tctcataatg
catatcaata tcttttgcac gtgctcccct tctccaacta gg 592230520DNAOryza
sativa 230atgcaaaccc actccaacac tccacaatcc acatactctc tctctctctc
tctctctgag 60taggagtaca tgtgtgtgtg tgatatcaat atgcattcga tgttgatgct
actgtagcca 120tcttgtggct atataaaccc agcaggcagc agcacagctt
agctagagag ccatattgca 180tgcacactcg ctaatctctt ttctctactc
tacttgcatt acaccacctc tgcattgcac 240ttcagttcat tcattccact
gatgcatgga tcgatgttgc taccttcttc tcttctcctc 300atgcatccat
gcatcgatct cacctagctt cttcctcatc ctctctcgat cgattacaag
360agaaaagtgt ttgctgttct tgctatcgat ctacaggtga gtaggttctt
gttggagaag 420cagggtacgt gcaaaatgca caccggttgg tcgagctaat
taacaagctc tgacgaccat 480ggtgatcgaa tgcacgtgct ccccttctcc
accatggcct 520231501DNAArabidopsis lyrata 231ctagggttta ggaatgacga
cttgtttctg ttgtgtctta ttaaaagccc atcttcgtct 60ccgccactca tcattccctc
atcataacac catcatcacc attcaccaac ctctctctct 120ttctctctct
cctctcgatc tacaacaaaa tgtgaatctg ctaagatcga ttatcatgag
180aattaagcta tttcagttga ggggaatgtt gtctggatcg aggatattat
acatatatac 240atgtgtatgt tgatacatgt gatcatagag agtatcctcg
gaccaggctt catccccccc 300aacatgttat tgcctctgat caccatatat
atgtcgttac atttcatggt taattacttg 360cacaaatcac aaaagcttgg
tttgtaactt tctatgacct tttttaatga ctttgaatct 420ttcatgcatg
acttcttaag agtagattta cacatttgcg gatccgttta tgctttttgc
480ttttgtttcg tttatatata t 501232484DNAArabidopsis thaliana
232ctagggttta ggaatgacga cctgtttctg ttgtgtctta ttaaaagccc
atcttcgtct 60ccgccactca tcattccctc atcataacac catcatcacc attcaccaac
ctctctctct 120ctctcctcta tcactctcta
caacaaaaat ttgtgaatct gctaagatcg attatcatga 180gggttaagct
atttcagttg aggggaatgt tgtctggatc gaggatatta tagatatata
240catgtgtatg ttaatgattc aagtgatcat agagagtatc ctcggaccag
gcttcatccc 300ccccaacatg ttattgcctc tgatcaccat ttattgttac
attttttttt gttaattact 360tgcgcaaatt acaaaagctt ggtttttgtg
atgactttga atctttcttg catggcttct 420taagagtaga tttacggatc
cgtctatgct ttttgctttt tgtttcgttt atttgtattt 480aaac
484233552DNABrassica carinata 233gagatcaatg aaattatcct gccaaataaa
acgtgtgacg tttattcaaa aatatatgca 60ttagatgctt tgatattaaa atatttcctt
ttaaaagcta gctagggttt aggaatgacg 120agttgtgtct tattaaaagc
ccttcttctc ctccgccact catcattccc tcatcataac 180accatcatca
ccattcaccc acctctcctc tttctctctc tctctctctc tctctctctc
240tagaacaaca agtgagaatc tgctaaaata ttgtgactat tatcatgaga
atgaagctat 300ttcagttgag gggaatgttg tctggatcga ggatattata
tatacacaaa tacgtatata 360tgttaataca agtgtttgat catatatgta
tatagattat tctcggacca ggcttcatcc 420cccctaacat gttattgcct
ctgatcacca gattctatca actcttcgct tattattttg 480tcacaaacaa
gtaataagct cataattttc tttgagtctt tcagcatcgt ttcattatgt
540ttttcgaatc cg 552234566DNABrassica juncea 234gagatcaatg
aaattatcct gccaaataaa acgtgtgacg tttattcaaa aatatatgca 60ttaaatgctt
tgatattaaa atatttcctt ttaaaagcta gctagggttt aggaatgacg
120agttgtgtct tattaaaagc ccttcttctc ctccgccact catcattccc
tcatcataac 180accatcatca ccattcatcc acctcttctc tcctctctct
ctctctctct ctctctctct 240ctctctctct ctctagaaca acaagtgaga
atctgctaaa atattgtgat tattatcatg 300agaatgaagc tatttcagtt
gaggggaatg ttgtctggat cgaggatatt atatatacac 360aaatatgtat
atatatgtta atatcagtgt ttgatcatat atatgtatat agattattct
420cggaccaggc ttcatccccc ctaacatgtt attgcctctg atcaccagat
tctatcaact 480cttagcttat tatttgtcac aaacaagtaa taagctcaat
aatgtctttg agtctttcag 540catcgtttca tatgttttcg aatccg
566235549DNABrassica napus 235gatatcaatg aaattatcct gccaaataaa
acgtgtgacg tttattcaaa aatatatgct 60ttaaatgctt tcatattaaa atatttcctt
ttaaaagcta gctagggttt aggaatgacg 120agttgtgtct tattaaaagc
ccttcttctc ctccgccact catcattccc tcatcataac 180accatcatca
ccattcaccc acctctcctc tttctctctc tctctctctc tctctctctc
240tagaacaaca agtgagaatc tgctaaaata ttgtgactat tatcatgaga
atgaagctat 300ttcagttgag gggaatgttg tctggatcga ggatattata
tatacacaaa tacgtatata 360tgataataca agtgtttgat catatatgta
tatagattat tctcggacca ggcttcatcc 420cccctaacat gttattgcct
ctgatcacca gattctatca actcttcgct tattatttgt 480cacaaacaag
taataagctc aataatgtct ttgagtcttt cagcatcgtt tcatatgttt 540tcgaatccg
549236543DNABrassica oleracea 236gggatcaatg aaaattatcc tgccaaataa
aaacgtgtga cgtttatcca aaaatatatg 60cattaaatgc tgtgatatga agtatttcct
ttaaaagcta gctagggttt aggaattacg 120agttgtgttt tattaaaagc
ccttcttctc ctccgccact catcattccc tcatcataac 180accatcatca
ccattcaccc acctctcctc tttctctctc tctctctctc tctctagaac
240aacaagtgag aatctgctaa aatattgtga ctattatcat gagaatgaag
ctatttcagt 300tgaggggaat gttgtctgga tcgaggatat tatatataca
caagtacgta tatatgttaa 360tacaagtgtt tgatcatata tgtatataga
ttattctcgg accaggcttc atccccccta 420acatgttatt gcctctgatc
accagattct atcaactctt cgcttattat ttgtcacaaa 480caagtagtaa
gctcaataat gtctttgagc ctttcagcat cgtttcatat gttttcgaat 540ccg
543237566DNABrassica rapa 237gagatcaatg aaattatcct gccaaataaa
acgtgtgacg tttattcaaa aatatatgca 60ttaaatgctt tgatattaaa atatttcctt
ttaaaagcta gctagggttt aggaatgacg 120agttgtgtct tattaaaagc
ccttcttctc ctccgccact catcattccc tcatcataac 180accatcatca
ccattcatcc acctcttctc tcctctctct ctctctctct ctctctctct
240ctctctctct ctctagaaca acaagtgaga atctgctaaa atattgtgat
tattatcatg 300agaatgaagc tatttcagtt gaggggaatg ttgtctggat
cgaggatatt atatatacac 360aaatatgtat atatatgtta atatcagtgt
ttgatcatat atatgtatat agattattct 420cggaccaggc ttcatccccc
ctaacatgtt attgcctctg atcaccagat tctatcaact 480cttagcttat
tatttgtcac aaacaagtaa taagctcaat aatgtctttg agtctttcag
540catcgtttca tatgttttcg aatccg 566238414DNAArabidopsis thaliana
238catcatcacc actcacttat cttcttctcc atctctctct ctgcttctcc
cttaatctta 60gccgggtctc gtgggggacg aacatagaaa gagagagata taaagatata
tattcagaaa 120ccctagattc tataatttcg actgaaaaga aaaaggggct
ttctcttttg aggggactgt 180tgtctggctc gaggactctg gctcgctcta
ttcatgttgg atctctttcg atctaacaat 240cgaattgaac cttcagattt
cagatttgat tagggtttta gcgtcttcgg accaggcttc 300attcccccca
attgttgctc cctgtttact ccatatttct tccttctttt caaattaggg
360tttcagatcc agtgaatgaa cccttgttaa aggtttgatc tcttacctta cttt
414239328DNAArabidopsis thaliana 239tctcatcatt ctcttcatca
tcaccacatt catctctctc tctctctctc tctctctctc 60tttctcttcc ttgatcttag
ccggatctgt tgggggacga acacatgaga gatagataaa 120atataagaaa
tttctcgaaa aaacctaata gaaaaaggtc tgtttcttaa agaagaagaa
180gaagaggatt taaagaggga tttttctttt gaggggactg ttgtctggct
cgaggactct 240tattctaata caatctcatt tgaatacatt cagatctgat
gattgattag ggttttagtg 300tcgtcggacc aggcttcatt ccccccaa
328240192DNAArabidopsis thaliana 240tgatgaacag aaaaatctct
ctttctcttt cttgatctgc tacggtgaag tctatggtgc 60accggcatct gatgaagctg
ccagcatgat ctaattagct ttctttatcc tttgttgtgt 120ttcatgacga
tggttaagag atcagtctcg attagatcat gttcgcagtt tcacccgttg
180actgtcgcac cc 192241846DNAArabidopsis thaliana 241aaccacaaag
taccgctgct attttctttt tacgtctttg tatttgcatc gtctaagaga 60atgatgggtt
gttttgtggg attttaatgc aggaggaaac atatgagggg tgattaaggc
120aaaaacctta agatgtggtc atttagatac atggagtcaa actaagaatg
gaccttggcg 180aaagcttctt cacggtcaag atttaaaatc aggtacgaca
ctgtgtacgt gagagagaga 240gagagagaga gaaagagatt atagaaagaa
agagatgtat cacaataaag gagtatattt 300agggtcacag gtggtggaga
tataggtatg cagggccaag gctctaatct cttcatagcc 360ctattgattt
tgtccctctc tctctctctt tcttcctctc ttagctgtat gcattatgat
420gcgtctttta attcactgtt tcaggcttct ttaattcgtg gtgtctctct
cctttttacc 480caaccatctc ttaaaatttt taacatctgt tcctcaaatc
ctctctcatc tctttctata 540agtatctata gcgcctctta aaccacaaag
catcacctct gtcttctctc atctcctttc 600tgtattctct ttcattgcct
tcacgtctgt tgcaatttct ccacttcttg agcttccgtt 660ttttacaatt
attgatccgt caaatatgtg agatttgcac aacttgttgc tcaggtattt
720tgaagacaag tccacaaggg aacaagtgaa gctgccagca tgatctatct
ttggttaaga 780gatgaatgtg gaaacatatt gcttaaaccc aagctaggtc
atgctctgac agcctcactc 840cttcct 846242571DNAArabidopsis thaliana
242gagcaagaca atgccacata caaacttgaa agatctcttc atcttttctc
caaatgtttt 60tttttcgttt gctatttatc tccacaattc ttggaacaaa aactacattc
acaaacgaga 120gaattttcac aacacctctt ttgctctcat tttttttttt
tcgtccatta tgagtattaa 180ttatggttag ggaatcttac agaatgaaaa
tgaaggtgtg aatggattgt ctcatctaaa 240gccttgaatg tgggaaaaag
gccattgttg ttcagccaag gatgacttgc cggtagcttg 300tattatgatt
actctatatt cgatttatat tatggagatg atggtttata tatatttact
360tatctacata gttttagttg attttttttc gtacgtaata taatacgaaa
aagtatttac 420ttatttatat atgtgtgttg gggcaagaag tgtaaccaag
ctagcccggc aagtcatcta 480tggctatgca actgtctctt cctctcattc
taggcttacg atgacacgta aaaaatccca 540aatatcacta atatgatatg
aatatggatg a 571243241DNAArabidopsis thaliana 243aggcatgaga
cataaagaga gttaaatata atgaagaaga gaggtctaat atggcgaaaa 60gagtcatgtt
taatagccaa ggatgacttg cctgatcttt ttcacctcca tgattcaatt
120ttaagttcgt ggattttgga ttattatgcg tttaaaaggt ataataattt
gagatcatgt 180tgaatcttgc gggttaggtt tcaggcagtc tctttggcta
tcttgacatg ctttcttcat 240c 241244689DNAArabidopsis thaliana
244gaattttgat ttatgaacct cctcaagaag gaaagacaga ggaggagaca
aagaagtata 60ggttcacatt gcatagccag tttagttttg aaggatggat atatgaaaaa
aatatgaaga 120gagagaagag agaagaagag gaggattaaa gagggtgagg
ccagcttttg tgctttggta 180gtagatgagg tttaaatgct ccataccttc
catttccttc tctcttaccc taatttaatt 240cttcctctcc tttataactc
cccacagaca ttctcacttc tcctcctcac acttcacatc 300aacacttctt
tcttgttttt tcattttaca atgtttcctt tgatatccgc actttaagca
360tgagagagtc cctttgatat tggcctggtt cactcagatc ttacctgacc
acacacgtag 420atatacatta ttctctctag attatctgat tgagccgcgc
caatatctca gtactctctc 480gtctctattt tggactttgt ggtcttgtag
atcgatttgt atgtgtgtgt tgaaatggag 540acaagtactt gtaacttctt
tgttgttata ttgtttacct ataggctgat gtcataaact 600cttttgatct
tgtttctaac ttccagattc ttgaaaaatc aagtcgtgtg tgtgtctcca
660tggaagcctt ttccatttct tcctttcca 689245452DNAArabidopsis thaliana
245actcataaac tttgctactg gccgcatttc tattttctcc ttcgattctt
ctaattcgta 60ctttggtttc tgacgtccct aaaattttta gacagtaaga gtttctccag
gatccgatgg 120ttctctccgg taaattaaca ttttagtgtc aatagtcatt
tatacatatt tttatttcac 180tttttgtttt gtttattggt tttctggagc
taagtggaga ttatagtcga acaagagtgg 240ttttatgcaa ggtaacgcga
gatattagtg cggttcaatc aaatagtcgt cctcttaact 300catggagaac
ggtgttgttc gattgagccg tgccaatatc acgcggtaaa ccaaaaatgg
360caaagatagt tattataacc ttaaaggtat gtatcattat cgttttattg
tttcaatttt 420gattaatggc tttgatattt catttttttt tt
452246767DNAMedicago truncatula 246attggtcaaa catacataca gtagcactag
ctggtttcat tattccacta tgcttcttca 60taggctctcc aaattttgca aaattgaaag
agacatagta tatatatctt agcaaggaga 120aattcaggat attgaggatg
aagattgaag agtaatcagt gatgaagaaa gcaagcaagg 180tattggcgcg
cctcaatttg aatacatggc tataaaaatg catcatatca gccatgtagt
240ttgattgagc cgcgtcaata tcttgtttcc atctccaaat ttaccaatct
catcaaatca 300aattaacacc acaatcaagg ctttcattta atgcagtcaa
aataggttga ccttatcatc 360gaagaaattg ttttctcatt cctatcgaag
ttggacttgc cgaaaatgct cgaaagcatg 420tgttttagtt cgacaggcga
aaaagttacc gaaggacaat ttggttgtgg ttcggataag 480atcaagcaac
ggatattttc aagacacgtt cgaaattcaa atcaaatgga taagtatcgt
540tagtttactg cagttatagt tttaaattca aatctaggca gttgtttcta
tttgtataaa 600tagtagtttt tccctaggga aaaggggtcg caattcaatc
atacaaaaaa cttacaatca 660aattatccgc atggaagaga gaaacgagtc
acaagttgca atgtatgaac atgtgtacca 720atttacattc aatcagtaca
atttaagttc atttccataa aaaaaaa 767247854DNAZea mays 247accagggtta
ggtatccatc cacacagcaa tgcatctgcc ttcaactccc tctcgccccc 60caccccaaca
cacatctctc tcttttctag ggaaggaaat gacgaagggg acgacgacgg
120catgcttcgg ctagctcgtt ggtgctagga caagggcgga ggtattggcg
cgcctcaatc 180cgaaggcgtg gctgatagat tggcgcggca gccatgttct
tggattgagc cgcgtcaata 240tctccccttg cctgtcccgt acctagctag
cttgcttgcc tcactgatcg atgtcgtccc 300tatttcatgg agaagctgat
gattgattat tctcacaagc aagaactgtc tgatctgttg 360cctgcatcga
tcaggatcta tatgctggag agttcacaag aacatggaca gaactcgctt
420caacaaccga tcaatcgatt gattaggtat gtacctacct catatgcctc
agctcttcgt 480tatggatttc ttcaaccgaa gggtcagtaa gctcttggtt
ccatgccact gcgtgaacta 540agcgttcaca aaatccgttc cmcggcatga
accaagcact caaaatcgca tgcagcatct 600ttcgtttcaa aaaaaaattg
acttytgaaa acaatagatg aatcagtttc aaacatatat 660gattatccat
tttctcaacc gggaatttat atctcgttgg gatgcaaaac cgttcagtag
720taaaactact ccacgagtat aaactgtttc agttatttta ctattaatta
gttacccgta 780tgctgttatg gtttctatat atctataagt aaaccttact
taaataagat agttatacaa 840aaaaaaaaaa aaaa 854248660DNAArabidopsis
thaliana 248caagaaaaaa cattgaaata gctcatgttg tctctttctc attttcatat
ctgctaaaaa 60aagaaccgtg ttttctaaac tggtttaacg gtaagtacct gtctctagta
acttacctat 120caatttgttc caatcattta cttgctttga cttatttggt
ttccttttgt tttgtttttc 180tttaatatgt ggatggagtt tggtgtaata
agcaactgaa gagtcgatga gcgcactatc 240ggacatcaaa tacgagatat
tggtgcggtt caatcagaaa accgtactct tttgttttaa 300agatcggttt
atttgattga gccgtgccaa tatcacgcgt ttaaatagtt taaagattct
360atgttagttg atgtgatcaa tcaaggtatg aatctatatc aattctctta
tgcatagttt 420tatatttaca gagatgaggt attatcaatg tctatcgtcg
aggatcacgc tcttacttat 480gttatatttc tatataattt tattaattag
ttttctaaaa gagaaggaca atttaaaatt 540attttaaaga gtttttttta
agtagttttg ttttcatgtt tatcttctgc aggctctgaa 600gttaggatag
taacaagaaa aaagacagaa aaaaagaaga aaattcatat acattcgtga
660249225DNAMedicago truncatula 249gaataagtga atattatcga tatttatatc
atatatcaac ttttcttctg tgcttgcttg 60caaatttgca attaagcttt tttgatctta
tgtaagagaa tattattgat gatggtgttt 120gggaagccga aaaaagcgat
gttggtgagg ttcaatccga agacggattt acatgtatag 180agttgtaaaa
tacgatctca gattgagccg cgccaatatc acttt 225250367DNAMedicago
truncatula 250ccacaaaact ataactagct agaagcttta atcgccttat
ttattataat aataataata 60aatatggctt cagctgcaaa agtatacatg gcgtgatatt
gatccggctc atctatatct 120tcaagttcaa tcatccatat tcatatcaat
ttcagacgag ccgaatcaat atcactcttg 180tttgcttcat tgcatattaa
ttatatactt catttataag ttatagtttg ccatatatat 240attagattga
ttctgcagaa gtagacagga gtggtgttgt ttctgctcat cttattaaat
300aatgaatgaa tgaatgacat ttgcttactt ataagacgag ccgaatcaat
atcactccag 360tacacct 367251712DNAArabidopsis thaliana
251ctctctctct ctctctctct catctgtgtt ctagatctca ccaggtcttt
ctctggttaa 60tatatggctt ccaagatctg gtaatatgtt ataaatacgt catacttaag
cttttttcaa 120atcaaaaata gaaatttgtg ggtttgtctc gttttactat
tttagcagta tatattaaga 180agttcagatg ttattcgatc atctgttttt
gcttcccctc tgccatcttt atcttttagg 240gtttcaattc tttttcactt
ttttcttctg gtttggagat ggttaggttc caactaagta 300tacgagatta
aatttgacat cttagttact tcaaaattcc ttcaatcaaa acaagtcatc
360tcgactattc cgccatgttt gtatatacat atttatatat tatatatatg
aaggtacgag 420tttctagtgt ctataaatta agaaggttaa gtaccatata
gatgatattt gttaagtagt 480aagtcactca aagtttgagt ttgggtttga
gtttgagttt gagtttgagt ttgagagaca 540aaagattact acaagaagat
tgttaaacaa aaatggaaga ctaatttccg gagccacggt 600cgttgttggc
tgctgtggca tcatcaagat tcacatctgt tgatggacgg tggtgattca
660ctctccacaa agttctctat gaaaatgaga atcttgatga tgctgcatcg gc
712252714DNAArabidopsis thaliana 252acttgcacct ctcactccct
ttctctaact agtcttgtgt gcacccattt atgtgtacgt 60actattatct cataaataaa
tatttttaaa attagatgca tttattgata tgaaaaagtt 120acaagattag
tttgttgtgt gtgagacttt ggatcgacag atcgaaaaat taactaaccg
180gtcagtattg aatatcaact attatatgct ccatgcattc gcttatagtt
tcacacaatt 240tgttttcttc acggtctaaa atcagaagat tccatatatt
ttcttatgac gtaaaaggac 300cacttataag ttgacacgtc agcccttgga
ttcgtgaggt ttttctctct acttcaccta 360tctacttttc ctcatatccc
actgcttttc tccttcttgt tcttgttttt ctcgtttttt 420tcttcttctt
ctccaagaaa atagagatcg aaaagattag atctattttg tgtagcaaga
480aattatcatt ttcgtttctt cattcatata ttgttctatt atgttgtaca
ataatagata 540ctcgatctct tgtgcgtgcg taaattttat acaagttgtc
ggcggatcca tggaagaaag 600ctcatctgtc gttgtttgta ggcgcagcac
cattaagatt cacatggaaa ttgataaata 660ccctaaatta gggttttgat
atgtatatga gaatcttgat gatgctgcat caac 714253324DNAArabidopsis
thaliana 253tcaccaaata ggctcttctt tatcgcttca tatatataaa agtctacatc
tatctctttc 60taggtcacta gctagactct agattaagga ttgaaattag ggtttcatgt
ttccagcaaa 120atggtgccgt cttgagtctt gaaaagatcc aagacaaaac
caaatcacta catacatccc 180tatcatcaac cagctactgt tcgctgttgg
agcatcatca agattcacaa atcatcaagt 240attcgtgtaa ataaacccat
ttatgattag atttttgatg tatgtatgag aatcttgatg 300atgctgcagc
tgcaatcagt ggct 324254531DNAArabidopsis thaliana 254tgtcatattg
agaactcttt agcctttggc ttctgttcct gacacttgta tagtgaagtg 60ggcttgtgtt
atatagatgg gatctctctc tttatttaaa agtcaattag agatcttgat
120gctacttctg tccctttcca agtgatttta cgtcgaccaa ctagcttttt
tcatatgagt 180gtatatattc atgtacctat ctctctcaat tgcttctcac
caaaatcatc ttgctgattc 240atttgctgtc tgaatcctct tgctttcctc
tttgcttttt catttgttga tttaaaccat 300gggagttccc aactttagac
ctcgaaaccg ataaggatct ttctctgcgg ttgaaatagc 360taggttctcg
atgaataggc tagcctttgg tggatgttat cagccagtag tcgcagatgc
420agcaccatta agattcacaa gagatgtggt tccctttgct ttcgcctctc
gatccgcaga 480aaagggttcc ttatcgagtg ggaatcttga tgatgctgca
tcagcaaata c 531255618DNAArabidopsis cebennensis 255ttgtatccat
agtgtatttc ctcgcatcta ccatccattt tctacgcctc tctctctctc 60tttctccatc
aaatcttgtt ttgttcaaac tctctctctc atcaattctc tccatacaat
120acatgcatac atacatacat accatctcta atatttcatc aatcttcttt
tgttccaaac 180gctctttctc tccatttaca tacatacgaa tcattgttgt
catagatccg tttagaattg 240ctttaacttt tagatgagat ctagggtttc
tttctttttc ttcaaaatca tgctttttcg 300cttgctaggt tatagatcca
tgtaagttta gagtagatgt acacacacac gctcggacac 360ttattaaata
catgttgata cacttaatac tcgctgtttt gaattgatgt tgtaggaata
420tataaatgta gagagagctt ccttgagtcc attcacaggt cggatatgat
ccaattagct 480tccgactcat tcatccaaat accgagtcgc caaaattcaa
attagactcg ttaaatgaat 540gaatgatgcg gtagacaaat tggatcattg
attctctttg attggactga agggagctcc 600ctctctcttc tgtattcc
618256660DNAArabidopsis halleri 256ttgtatccat agtgtatttc ctcgcatcta
ccatccattt tctacgcctc actctctctt 60tctccatcaa atcttgtttt gatcaaactc
tctctctctc tctctcatca attgtctcca 120tacaatacat acataccatc
atctttccca tctctaatat ttcatcaatc ttcttttgtt 180caaacgctct
tcctctccat atacatatac atacatacga atcacattgg tgtcatagat
240ccgtttagaa ttgctttaac ttttagatga gatctagggt ttctttgttt
ctttcgtttt 300cttcaaattt tgctgcatat tctccaagat catgattttt
cgcttgctag gttatagatc 360catgcaaata tagagtagat ttacacacac
acacgctcgg acacttatta catacatgtt 420gatacactta atactcgctg
tttttaattg atgttgtagg aatatatata tgtagagaga 480gcttccttga
gtccattcac aggtcgtgat atgatccaat tagcttccga ctcattcatc
540caaataccga gtcgccaaaa ttcgaactag actcgttaaa tgaatgaatg
atgcggtaga 600caaattggat cattgattct ctttgattgg actgaaggga
gctccctctc tcttctgtat 660257632DNAArabidospis lyrata 257ttgtatccat
agtgtatttc ctcgcatcta ccatccattt tctacgcctc tctctttctc 60catcaaatct
tgttttgttc caactctctc tctcatcaat tcattccata caatacatgc
120atacatacat accatcatca tcttttccca tctctaatat ttcatcagtc
ttcttttgtt 180acaaacgctc tttctcgcca tatacataca taagaatcat
tgttgtcata gatccgttta
240gaattgcttt aacttttaga tgagatctag ggtttctttc tttttcttca
atttttgctg 300catattcttc aaaatcatga tttttcgctt gctaggttat
agatccatgc aaatatagag 360tagatgtaca cacattcacg ctcggacact
tattacatac atgttgatac acttaatact 420cgctgttttg aatggatgtt
gtaggaatat atatgtagag agagcttcct tgagtccatt 480cacaggtcgt
gatatgatcc aattagcttc cgactcattc atccaaatac cgagtcgcca
540aaattcgaac tagactcgtt aaatgaatga atgatgcggt agacaaattg
gatcattgat 600tctctttgat tggactgaag ggagctccct ct
632258646DNAArabidopsis thaliana 258ttgtatccgc agtgtatttc
ctcgcatcta ccatcccttt tctacgcctc tctccctctc 60tctctttctc catcaaatct
tgttttgttc aaactctctc tctctcatct attctctcca 120tacaatacat
gaatatacat acataccatc atcttctttt cccatctcta gtttttcatc
180aatcttctga tgttccaaac gctctatctc ttcatataca tacatacgaa
tatattattg 240ttgtcataga tccatttaga atcactttag cttttagatg
agatctaggg tttctttgtt 300ttctttcaaa ttttgttgca tattcttcta
aatcatggtt tttcgcttgc taggttatag 360atccatgcaa atatggagta
gatgtacaaa cacacgctcg gacgcatatt acacatgttc 420atacacttaa
tactcgctgt tttgaattga tgttttagga atatatatgt agagagagct
480tccttgagtc cattcacagg tcgtgatatg attcaattag cttccgactc
attcatccaa 540ataccgagtc gccaaaattc aaactagact cgttaaatga
atgaatgatg cggtagacaa 600attggatcat tgattctctt tgattggact
gaagggagct ccctct 646259660DNABrassica rapa 259ttgtatccat
tgtgtatttc cttgcatcca tcaataaatt ttatgttacg cctctctatt 60atttctctct
acatcacact gtcttatgtt taagctctac ttctcagcaa ttctctccac
120ccaatacatg catacatacc atcatcgtat cgctctaatt tttctatcaa
tcttgtatcc 180ttccacaaat tatcttatgt ctcccatttt aaatcctaca
tagatccaca catacgaatt 240attcttgtct gaagatccat ccatttacga
ttgctttaac ttttacatga gatctagggc 300ttctttattt ttcttcaaat
cttgctgcat atatttcaag atcatgcttt tcggcttgct 360agggttctag
atccatggat gtatagcgta catacataca cgcactaatt catacctgta
420gtttgtacgg agaacatcat aaaatatcac tgtttggaat taatcgtgta
ggaaatatag 480ataggtaggg agcttccttt agtccattca cagatcatga
tatgatccaa ttagcttccg 540actcattcat ccaaataccg agtcacgaaa
atttcaactt agactcgtta aatgaatgaa 600tgatgcggta aacaaattgg
atcattgatt ctctctgatt ggactgaagg gagctccctc 660260932DNACarica
papaya 260ctatatccct gtcaaatagt acttggtttt gttttagcca caaatcttct
ggtcttgcaa 60agttccttct gatctctccc cctttctcat tttttcctcc tcttatatat
ggatcatcaa 120tttgctgtac acacacacac atttactgta gtgataatta
gctagctaat ttgttagtat 180gtaaattaga tcccaagtac cctgttatat
ttttttaggc ttatcctatg catacctgat 240agtacaagaa cttagtttgt
aattaggtac ttggtagtag ggttagatta attactgtct 300tgaaagagaa
cttatccaac aaatagagct atgaagatta aattaggttt tagtcttatt
360aagattatta tattactaga caaaaacagt taaatttttt taattgggta
attaggtact 420tagcaatagg gttagattaa ttactgtttt gaaagagaac
ctacctacaa atagagttga 480aatgattatg ttttagtctt actaagattg
tcatatttct ggaaaaaaac aaatcttgaa 540acagataatt cagatagtca
tgatcaatgg aaaaaacatc atgggtgtgt gcttaattaa 600gctaatatat
atatatatga agatataatg ttatgcacac tagctatgaa tttgtaagaa
660taatgaagga taaagatgat atatttagat gttataagtg taagtaaggt
ggaatgggtt 720gatgggtagt agtagtagta gtagagatga ttggtggaga
gagcttcctt cagcccactc 780atggatgggt atgaaggggt agaagtagct
gccgactcat tcattcagcc actcagtatg 840taaactcgtc ccactgttga
ctgtatgaat gatgcgggag atatttttac atccatcttt 900ctctgtgctt
ggactgaagg gagctccttc tt 932261702DNACapsella rubella 261ttgtatccat
agtgtatttc ctcgcatcta ccatctacta ttttctacgc ctctctctct 60ttatccctct
atctctttct tcatcaaatc ttcttttgtt caaagtctct ctcatcattt
120ttctctatac acatacatgc atccacatac atacatacat ataccatcat
cttcttttct 180catctctagt ttttgtttat aaattttgtt ccaaggatct
gtatctctcc aataaagata 240catacaaatt attgttgtca tagatctatt
agaattgctt taacttttat atgagatcta 300ggatttcttc cttttctttc
aaaatttgct gcatattttt caaaatcatg atttatcgct 360tgctaggttc
tagatccatg caaatttaga gtatttttac acacacacac gcttggacac
420aagtacatac atgtagtttt cttttatgtg gtgaaaagta cataacatgt
agtttatagt 480tactagtcgc tatataattt aaaattgatg ttataggaat
atatgtacgg agagagcttc 540cttgagtcca ttcacaggtc gtgatatgat
ccaattagct tccgactcat tcatccaaat 600accgagtctc accaaaattg
gaacaagact tgttaaatga atgaatgatg cggtagacaa 660attggatcat
tgattctctt tgattggact gaagggagct cc 702262767DNAEucalyptus grandis
262tcgtttccca tccccatttc atagaataat gccaccaaac aaagaagcat
tagctcaaag 60actaattacc atctgtttta ttgatagata cgtgcgaaac ggtgattgtt
ttttcccaaa 120taagaaacca aaatgaagca tattcaaagg tggagatatg
gggagacttc cggaaggcaa 180ggggattgga aaaggctcga gatcaaagtg
catagcaacc cttcgctaaa ggtgaaaaag 240aatacgaata acttcagtag
ctcactttaa attccgaaac attaaacaaa tcaaatctcc 300ctcgccctcc
ttgcctcctc tctttaccta tataaagcca ccgccccttc aatgaaatcc
360acgagtggaa ggtcacagta tagtagggtc ctgcaaaggg agagcgagag
cggctccact 420gtctacctat aagcagttcc tttcttttgt ttacatgtct
gttgcacctc accgagtttt 480tctattctct ttcctctggt tggttagcag
atttctcagg ggacttccct cctcccttga 540ggatccttct cttgaagcga
tatgtctcga atgggtaaga gagagagagg aagggagctt 600tcttcagtcc
acccatggga cgtgttgggt tttaattagc tgccgactca ttcatccaaa
660taccgagcga gagcaagtaa cagagctccg taaatgaatg gatgatgcgg
gagtcttgtt 720gattcccaag ctttccgtga ttggactgaa gggagctccc tctatct
767263612DNAGossypium raimondii 263gcgtatcctt ccacttacgg attcaccata
gctgctatag cccgagtttg cttgtcataa 60tagaaataaa ctaagggaga aaaaagctct
ccactctcgc ctttttcttc ttcgagtctc 120tctctttgac tggcctttgt
gcaaagatct tcctttttta aacagtcgct ttctttactc 180tccctttccc
ttctttctct taattgctaa agcagcttcc acttccactc actttaaccc
240atgatccatt tcaacctgtc acagtggaga gcaatttgta tggctaattt
ccatctcacc 300tattcttttc tgtttggggt tctctagtct agggttgcat
gacatgagag acatggctct 360tttttttttt tttcagttca caggtgttta
tatatgttgt tgtgttattt tgtcttaaag 420ctttgtgatt gatgatctga
taggtaagag agagctttct tcagtccact catgggatgg 480ggatggggtt
taattagctg ccgactcatt catccaaata ctgtgttaca aaacccagta
540aatgagtgaa tgatgcggga gacaaattga atcctaatct tcctgtgctt
ggactgaagg 600gagctccctc cc 612264675DNAMedicago truncatula
264atttatccaa tcatgttgct ctcacttctt ttcagcagtt ctgttgatat
caccctttta 60gttgaagctt tggattcatg actttatgag atggtaaatc tttaaattaa
gtttaagtat 120gcacttgatt tgtttatata atttgtttat ttagatttta
aaccctaagt tgactttttt 180aatttgattt aaattatgat atgattgtta
tttggcttac catggtcata atttagggtt 240tagaagatgc atgtatatct
tgaattgttt atggtaataa aggggttagg atttctcctt 300ttggtgaagt
gagaaaatct cataattttg ttctgaaggt agtttttaag atttagggtt
360atgggttctt tgtttgaatg ctttttcaag tctttttcaa tgatatttgc
ctagatctgt 420tttaattttg aattaaaatt ctgggtttgg attaatatta
ttgaagatta ttattaatta 480atttattaga aatagatgaa gagagcttcc
ttcagtccac tcatggaagg gtaaggggtt 540tgaatttacc tgctgactca
ttgattcaaa cacaatagac aattatgggg ttatgctatt 600gtgaattgtg
tgaatgatgc aggaggtgaa tttcttcctt ttcttctttg cttggactga
660agggagtctc ccttt 675265866DNAOryza sativa 265ttatatctga
cgcgttgtaa tcctgtttaa ttagggcttt gcccatttct tttgacccct 60tccggacatt
cgctagttgg aaccttgttt tactcctagc agtgtactgt gtagtactta
120ttacgagcaa acgtaaaaat aaataaatgg aaatgataca aaggccgtgt
ttaattttaa 180aatttttttt caaactttca acactttaca tcccataaaa
agcttactat acatacaaac 240ttccaacctt ttcgtcacat cgtatctaat
ttcaactaaa cttttaattt tagcgtgaac 300taaacacagc cgaagcccgg
ccacattctc actattttta ttcattttat catgcctgtg 360atgtcacgcc
ttggccctat ttaataggcc ttctccattt ctctccatat gatgtcttct
420cttctctatc cctcttgcca tcttctatct tccctcttgc acccatcttt
gtgataactt 480ctactagctc ctctcctact accagtcata ccactctcac
aaatcctcca agatccgcat 540ggggagaagc tccaaaagtt tcgtggttag
tttaatttca tgcttgtttg ctgccgtttt 600tcatgttgat ctgatcttaa
tatatgtaga ctgctgttaa catattcttt taatttgatg 660gaagaagcga
tcgatggatg gaagagagcg atccttcagt ccactcatgg gcggtgctag
720ggtcgaatta gctgccgact cattcaccca caatgccaag caagaaacgc
ttgagatagc 780gaagcttagc agatgagtga atgaagcggg agagtaacgt
tccgatctcg cgccgtcttt 840gcttggactg aagggtgctc cctcct
866266801DNAPhyscomitrella patens 266tgggatccac acgagtaatt
attccctctc tcatgcatca caacttggac ttgcccagct 60tttacctctc tccatgttcc
accgtcggag atcctcggtg ctgctacccc cgttcggcca 120aacccaaccc
aaccctaggt gtctgccgga cctccgcttc ccctcctgct tcaccccctg
180caccgcttaa atttgcacct cgtagtattt atctatgccc catttcagat
gacttgcatg 240acacccatct ggattcgtct ccaggcgcct tgtcgtatct
attcgctatc agtctttctt 300tcagtctgtc tttctgccaa gcacaccgtg
ctggtggtgg tgctatcagt cgaagcagtc 360gccgaagggt ccttgtatcc
agccctcacc ggagacagtt cgcttcgggg tggggagaac 420tgttgagacc
gtcgatgttg catgcagcag cactggcgaa ggtgcttctg atactgggta
480ttccagcctc gccgctcggt gcactgcagg tacgtggtta catgacacta
ctgtgtgggt 540agtctgggca caagtagatc gacatgccag atttggcccg
tatgcgtgta tgtgcggtta 600tgttcccgtt tcgattgcgg ataccttgat
tgtggagctc cgtttcggtc caatagtggc 660tgcgacggaa ggtggtcccg
ctgccgaatc acacgtccgg gttgcttatc ggggcagggc 720cccgatacgg
tatccgaacg tttgtcccgg gaactggtcg accttccgcc cggcgtctct
780tggactgaag ggagctccac t 801267819DNAThellungiella halophila
267ttttatacaa ataatgttcg ataacactaa accctagcca tccaactaat
agacaaaacc 60ctacttgtaa tttacaaccg caaattccca gagaacagag taactacgag
agagagatgg 120agattcaaat taaaaagaaa aacttatata taatgaatac
acaaaagcta cctaatctgt 180atatatatat atataaatat gtcttcatta
aattaatggt cgtggaatag aaaaaggaaa 240acctaatttg atcgctaggg
cttatcagag taaagatggt taaccttcaa aagatgacta 300attaaccggg
gagataatta aaagattaaa tacgccaaca gagagttaag agataccaga
360tttaaattcc acaatttggt catgttcttc ttcacgtatt catgacgatg
tctgaattat 420agagaaaccc aaaatataaa atgttaattt taccagacat
ttacatacca ataactctat 480gacgatatgt aaagtaagca aggcatgttt
ttatgcaggg aagattgaaa attcaagatt 540aatcaagaaa attggaatac
caaaaagaga gggagctccc ttcagtccaa tcagagagaa 600tcaatgaccc
aatttgtcta ccgcatcatt cattcattta acaagtctag ctcgaattct
660tggtgactcg gtatttggat gaatgagtcg gaagctaatt ggatcatatc
acgacctatg 720aatggactca aggaagctct ctacaaatgt attcctacca
catcaaccca aatatagtga 780ttacagatgc tgttctcact gtagactaca tttacgttt
819268503DNAArabidopsis thaliana 268agacatctct tcttctctca
tctctctttt cttctctctt ttcctcacat aaactctctt 60tttttactat taaatccata
tggtacctca aattaatcta tggtcatcta gggttatctt 120gaagattaga
attgattcta gcacgcacag agaggaagat cattgcatcc agaatcacaa
180acatggccta tcttttatct tttctttttg atctaagtca ctgttttatg
ctatatatag 240tataatcaaa ttctttacat gtgcttgtat gtatgcgtat
atatagtaac ggaattgtta 300atatgcttat agatgttgag ttggtggagg
aagagagctt tcttcggtcc actcatggag 360taatatgtga gatttaattg
actctcgact cattcatcca aataccaaat gaaagaattt 420gttctcatat
ggtaaatgaa tgaatgatgc gagagacaaa ttgagtcttc acttctctat
480gcttggactg aagggagctc cct 503269365DNAArabidopsis thaliana
269tcttattcca tcacaatcat ctagggtttt aagccaagct tatatagccc
gtcataaaga 60gaactcatct gcctctctct caataccaat aaatatcacc accgtccttc
tctcctatca 120ctattcaatc tatcgcaaac tcctttatgt ctctccaatt
ttatgagagg gtttccttca 180agaacacagt aaaatagatt ggatctttaa
acttttgttc cttttcatga gggtttgaca 240aagattttct tacagtcatc
tttggcattc tgtccacctc cttctataca tatatgcatg 300tgtatatata
tatgcgtttc gtgtgaaaga aggaggtggg tatactgcca atagagatct 360gttag
365270134DNAArabidopsis thaliana 270ttgtatcatg acagagcaag
aagaagaaag tcaaatgtcc acatgagttc cctttaacgc 60ttcattgttg aatactcaaa
gccacattgg tttgtatata acactgaagt gtttgggggg 120actcttggtg tcat
134271229DNAArabidopsis thaliana 271tttcaaaccc taacactctt
ataaaccgat tcgccaaaat gtatctacaa tatattgata 60atgtaatatc tatatattca
aacaatcgtc gtgttggtcg gatgttttct agagttcctc 120tgagcacttc
attggagata caatttttta taaaatagtt ttctactgaa gtgtttgggg
180gaactcccgg gctgattcgg tattttaaat tcagtagact agctagctg
229272220DNAArabidopsis thaliana 272tggtaataga aatgagcaag
gagatatttt tttcccctgg gtttgaatga acatcattga 60gtgcatcgtt gatgtaattt
tacttatttt attccattgt tgaattaatt aaagaagtat 120atatcagcgt
tgcattcaat tatgtttttc taattttcag gaaatacaaa aaaaatgaaa
180aaaaaaaatc acttaaaaga ccttgagagt tcttttgact
220273225DNAArabidopsis thaliana 273ggatatcgaa actcaaactg
taacagtcct tttattactg gtttagaaga tagataaata 60ttgttaaggt agtggatctc
gacagggttg atatgagaac acacgagcaa tcaacggcta 120taacgacgct
acgtcattgt tacagctctc gtttcatgtg ttctcaggtc acccctgctg
180agctctttct ctaccgtcca tgttttatca acgccgtggc ccgtg
225274165DNAArabidopsis thaliana 274tcttatagag atgaagagaa
acatgtaaac tcactagttt tagggcgcct ctccattggc 60aggtccttta cttccaaata
tacacataca tatatgaata tcgaaaattt ccgatgatcg 120atttataaat
gacctgccaa aggagagttg ccctgaaact ggttc 165275187DNAArabidopsis
thaliana 275caataactca aaatgcaatg tgaaatatga agaatatatt aaatagtagt
gaagatgcat 60gtttatgaag acagagagat aatgtatggt tggattactg ggcgaatact
cctatggcag 120atcgcattgg ctagatatgc aagtaaaatg cttctctgcc
aaaggagatt tgccccgcaa 180ttcatcc 187276235DNAArabidopsis thaliana
276atttaggtct ctcttcttct tcttcttttt cttcttgagc gccggcgaaa
aaagtctctg 60tgagaaaaag atacgacgat tgtcattaga agagtcgtat tacatgtttt
gtgcttgaat 120ctaattcaac aggctttatg taagagattc tttaacaatt
cctataatct ttgttgttgg 180attagattca cgcacaaact cgtaatctgt
cttttcgatt tttaccagat ctgtc 235277944DNAArabidopsis thaliana
277aattatatcc atggtcatgg ctcatcatta gtcgcactgc tctccttttc
tcaaagttta 60aattcgacat ttggtaaaat gatgaaacct cgatggaact gctctcttta
tggaatcacg 120gaatggacaa ataatcaaaa tcagaaatcg aagcgaaaag
ggaggagaaa aacgcagatt 180tggaggattg gggacagatt agatactgtt
gaatgcatca ctctaatgct atcagcctat 240taatagcgtc ctatattttc
gaagactttt aatgtttagg gttatggatt tttcgagcga 300agcatggaga
gatgttgaat tggatactat aggatttggt acaacacata catatgttct
360gcttctgcaa aactaacata tcaagttcag agaaaccagt aagtcgttga
atattttatt 420atccattcaa cgctttcttc ttttggatca tgtcttgttt
gcttgaccac ttcttcttgc 480ttaagaggat ggacaatata taaaaactgg
agccttcttt ttctatgaat gcttatcatc 540gcggagttga tctgttcaat
tcacctgcca ttggatgctt tttttatata tacttcactg 600ttcaatttca
gatgctttag aaggtttgcg gagtagctag agaatctggt atcttcagtt
660cttcaatttc agctacttgg tatcagcttc gtcattgtat atcaacacat
tcttaatata 720taatactact ttttcatcca ttaaacccct tacaatgtcg
agtaaacgaa gcatctgtcc 780cctggtattg tcttcgagct tggtgttttt
ttctagccaa ctccaagttc tcgagttgat 840cattgtttgt attcttgaga
cattatttgg ggacgagatg ttttgttgac tcgatataag 900aaggggcttt
atggaagaaa ttgtagtatt atatatcgag agtg 944278420DNAArabidopsis
thaliana 278ctataaatgc tgcttatcat cgtggagttg gttctgtaaa catttgaaaa
ttctgaacag 60tttcacctgc cattggatgc tttgtttcaa tttcaggtgc gttagaaggt
ttgcagagta 120gctagagaat ctcgtatctt cactttctgc tacttggtat
cagcttcgtc actttatatc 180aacacattct taatatacaa tactactttt
tcatccatta atccccttac aatgtcgagt 240aaacgaagca tctgtcccct
ggtattgtct tcgagcttgg tgtgtttttc tagccagccc 300caagttctcg
agttgatcat tgtttgtatt ctgacacatt atttggggac gagatgtttt
360gttgactcga tataagaagg ggctttatgg aagaaattgt agtattatat
attgagaatg 420279383DNAArabidopsis thaliana 279tagtataacc
gctgatgtac acctaccagc ttgataactc tttttcgtgg tttctgtgta 60ctcgtttctg
tttgtacaga tacttcttgt tcaatttcag atgctttaga aggttttcgg
120agtggctaga gatctgttat ctgtatgaac agctacttgg tatcagcttc
gtcattttat 180caacacattc ttaatataca atacttcttt ttcatgcatt
aagcccctta caatgtcgag 240taaacaaagc atgtgtccgc taatattgtc
ttcgagcttg gtatttttgt attctgatac 300ggtatttggg gacgacatct
tttgttgact cgatataaga agggggtttg tggaagaaat 360tgtagtatta
tatatcaaga atg 3832802164DNADrosophila melanogaster 280tttgtggaac
acattcgacc cactgaaaaa ttgatataat ttaatgaaag tgcataaaaa 60tggtggacag
tgcattaaac tgagcattga acacaaaggc cgctcagcaa attgctaatt
120aaaattcacg attgccattt cacctgacac gttgacgatt ttcattacaa
ttcgattatg 180tttcgttgca gggaatttta aatgttaatt gccaagaatg
tttcaacaaa ttcatttctc 240attaatgtgt cttttcattt aattttatgt
tgtatgagct gcacgagaaa tgagttgtac 300ttttagttcg acggcagagt
catgaatgtt cggcaaagaa tgtaataata actatcctct 360ttagacaaat
atagatacaa atctatcaga ttctaaaagt agaataatca attaatcaga
420aagctaaaaa taaataggca tatttatatt ttaatgcgga tttttgaagt
tcaacgggag 480aaatgaatcc tttttaccag ccacaggcgc aatttgcaac
agaaagtgta gcagaagtac 540tcctcgaata tttccctgct ccaggagtca
tccatgtggt ttcgaggcac acatttgaca 600aactcatgcc ccgctatttg
ttgtaaaaac acaatcgcac acatggccgc atttcggcga 660cttccagaga
gcggtacact taaggcggcc tgggaaacgc ctgcaatctg ctggtcgcga
720actgcagatt gcatccatgt gccaggcgac catgcgacca tgtgaccatg
tgcccgcccg 780acgcctcgca gcccacatcc tgcccatcga gggcacaact
cagcgtgggt attgccgctc 840cggctgcttc aagtaggtaa aaaccgagaa
gattgaggat gaatgtatga gtatgagaaa 900atactcggcg gaacatatgc
tgccgggctt gacctgaccc tgcctcatgt gtgggtctcc 960gatttaattt
taggcaccta tataaacgcg tgtttacact gcagccagaa cacagtcgcc
1020gtcttcagtt cgcgccgtca actcctcgat cgatcgatcg catcgtctcg
gatcgaatag 1080agctgggctt ctgctccgga gctacatcgc cgtacttgtc
ggacgagtgt ggtgatgaaa 1140agtcgcttag tccgggattc ctgccagatc
tctaagggat gagctggcat cccaggctgg 1200ccatgtggcg cgaagtatgc
gcacaaaaaa gtcaaacaaa aaggcgcaat tttattacgg 1260gcaaccaacg
acgaaacaaa acaaaagcca accgaaaagc agaaacaaag cagcaaaaag
1320tttatgaatt ttttgtgcag gcgcgtgaaa gatgcaaaac gagaaaaaaa
catgaaaaaa 1380aaacattaaa aaaaacaaaa aaaatccaaa acagataccg
agctgtatcc gaaaacgagt 1440ggggaaaggg gtttcccagt cacatataaa
cacacttcag tgcgcttaaa aattgcttta 1500ttgcagttgg actataaaaa
cgcacggcag cgaacaccgc acaacaaaaa ggacgagcag 1560aagtgggcaa
ataaaacgaa agctcttaaa cgaaaaacag gaaaatttgc atgccacaaa
1620aataagcata aggatttgcc gcgcacaaag tagaagcaaa aaggaattgc
ccaaatgcag 1680ccacaaaaga ctgtggcaaa tgttttgcag cttgcccctt
tttccctgca attaccgtca 1740gtcgttgtca ttattcagca gattatatgg
ttttgcttat tccggaccac catcatcatc 1800atcattatca tcatcttcgg
taagttagac aatcccataa aaaactgtcc aagtgagtag 1860tgccaccaaa
agttagccgc gttgtggaaa
atccaaaaca aagaccatcc catattcagc 1920ctttgagagt tccatgcttc
cttgcattca atagttatat tcaagcatat ggaatgtaaa 1980gaagtatgga
gcgaaatctg gcgagacatc ggagttgaaa ctaaaactga aatttgattg
2040aaacagaagt agaaccgtaa tgaaatgaat gaaatattaa cccgtttcta
caatccctga 2100ataaaattat taattaatta tagagcgggc taattttaca
atatatattg attttttttt 2160gaag 2164281919DNADrosophila melanogaster
281attctttttt ggtgctcgat cgtgacggtt tgctcgcgct ctccgctgcg
ccgctctttc 60cgttgcatat gtgtgcgggc gttattgtgc atgtttccgg tggccgaaaa
aaaatagtaa 120aataaaatat agaaaacaga aaccaagaat aataacagcc
atacgataaa cagtgtgcca 180atgtgtgtgt ctgtgtgtgt gtgcatctcg
cgtaacaaca ataattgcat ttatcggatg 240gcgccagctt caatttaatt
ataaataaca tgttcaactt tttatactat tttccctgcg 300tcaaagtggg
cgttgcaact gcccccggaa aatcacgcgc cccggttcaa agttaaagtt
360tgctgggtaa cgcacacaca cacacacaca atcactcaca cgcggtcaca
cgcacatttc 420aataaactaa tggagcctgg ctttgttttt gttttatttc
caacccactt gagcacacag 480cacacacaga gagaaaaatc aatactcgtt
atgggattaa atttacaaag cgcaaagcaa 540agcgacaaac aaaattcaaa
agaaagaaaa aaaaacactc aaataaactc acaaagaatt 600ccttatcgcc
aagggggcca atgttctaag gttctttcgc cttgagaact ttgagcttcc
660tctggcaaag gagattataa tgtacaaata atgttgcaat aaccagttga
aaccaatgga 720ataccgaatc ttgctaatta gcaaggacat ctgttcacat
cttaccgggc agcattagat 780cctttttata actctaatac tgtcaggtaa
agatgtcgtc cgtgtcctta accttcagta 840ccaccaacag cagcagcagc
accaaaaaaa aaaaaaaaaa aatgcgtaaa aatccaaaca 900aatcataaaa gtcgaagga
91928220RNAArabidopsis thaliana 282ugacagaaga gagugagcac
2028320RNAArabidopsis thaliana 283ugacagaaga gagugagcac
2028420RNAArabidopsis thaliana 284ugacagaaga gagugagcac
2028520RNAArabidopsis thaliana 285ugacagaaga gagugagcac
2028621RNAArabidopsis lyrata 286uuuggauuga agggagcucu a
2128721RNAArabidopsis thaliana 287uuuggauuga agggagcucu a
2128821RNACapsella rubella 288uuuggauuga agggagcucu a
2128921RNAArabidopsis thaliana 289uuuggauuga agggagcucu u
2129021RNAArabidopsis thaliana 290ugccuggcuc ccuguaugcc a
2129121RNAArabidopsis thaliana 291ugccuggcuc ccuguaugcc a
2129221RNAArabidopsis thaliana 292ucaaugcauu gaaagugacu a
2129321RNAArabidopsis thaliana 293ucgauaaacc ucugcaucca g
2129421RNAArabidopsis thaliana 294ucgauaaacc ucugcaucca g
2129524RNAArabidopsis thaliana 295uugaagagga cuuggaacuu cgau
2429621RNAArabidopsis lyrata 296uggagaagca gggcacgugc a
2129721RNAArabidopsis thaliana 297uggagaagca gggcacgugc a
2129821RNABrassica rapa 298uggagaagca gggcacgugc a 2129921RNACarica
papaya 299uggagaagca gggcacgugc a 2130021RNACapsella rubella
300uggagaagca gggcacgugc a 2130121RNAGossypium raimondii
301uggagaagca gggcacgugc a 2130221RNAMedicago truncatula
302uggagaagca gggcacgugc a 2130321RNAOryza sativa 303uggagaagca
ggguacgugc a 2130421RNAArabidopsis lyrata 304ucggaccagg cuucaucccc
c 2130521RNAArabidopsis thaliana 305ucggaccagg cuucaucccc c
2130621RNABrassica carinata 306ucggaccagg cuucaucccc c
2130721RNABrassica juncea 307ucggaccagg cuucaucccc c
2130821RNABrassica napus 308ucggaccagg cuucaucccc c
2130921RNABrassica oleracea 309ucggaccagg cuucaucccc c
2131021RNABrassica rapa 310ucggaccagg cuucaucccc c
2131121RNAArabidopsis thaliana 311ucggaccagg cuucauuccc c
2131221RNAArabidopsis thaliana 312ucggaccagg cuucauuccc c
2131321RNAArabidopsis thaliana 313ugaagcugcc agcaugaucu a
2131421RNAArabidopsis thaliana 314ugaagcugcc agcaugaucu a
2131521RNAArabidopsis thaliana 315cagccaagga ugacuugccg g
2131621RNAArabidopsis thaliana 316uagccaagga ugacuugccu g
2131721RNAArabidopsis thaliana 317ugauugagcc gcgccaauau c
2131821RNAArabidopsis thaliana 318uugagccgug ccaauaucac g
2131921RNAMedicago truncatula 319ugauugagcc gcgucaauau c
2132021RNAZea mays 320ggauugagcc gcgucaauau c 2132121RNAArabidopsis
thaliana 321uugagccgug ccaauaucac g 2132221RNAMedicago truncatula
322agauugagcc gcgccaauau c 2132321RNAMedicago truncatula
323cgagccgaau caauaucacu c 2132421RNAArabidopsis thaliana
324agaaucuuga ugaugcugca u 2132520RNAArabidopsis thaliana
325gcagcaccau uaagauucac 2032621RNAArabidopsis thaliana
326agaaucuuga ugaugcugca g 2132721RNAArabidopsis thaliana
327ggaaucuuga ugaugcugca u 2132821RNAArabidopsis cebennensis
328uuggacugaa gggagcuccc u 2132921RNAArabidopsis halleri
329uuggacugaa gggagcuccc u 2133021RNAArabidospis lyrata
330uuggacugaa gggagcuccc u 2133121RNAArabidopsis thaliana
331uuggacugaa gggagcuccc u 2133221RNABrassica rapa 332uuggacugaa
gggagcuccc u 2133321RNACarica papaya 333uuggacugaa gggagcuccu u
2133419RNACapsella rubella 334uuggacugaa gggagcucc
1933521RNAEucalyptus grandis 335uuggacugaa gggagcuccc u
2133621RNAGossypium raimondii 336uuggacugaa gggagcuccc u
2133722RNAMedicago truncatula 337uuggacugaa gggagucucc cu
2233821RNAOryza sativa 338uuggacugaa gggugcuccc u
2133920RNAPhyscomitrella patens 339cuuggacuga agggagcucc
2034020RNAThellungiella halophila 340uggacucaag gaagcucucu
2034121RNAArabidopsis thaliana 341uuggacugaa gggagcuccc u
2134220RNAArabidopsis thaliana 342uuggcauucu guccaccucc
2034321RNAArabidopsis thaliana 343cugaaguguu uggggggacu c
2134421RNAArabidopsis thaliana 344cugaaguguu ugggggaacu c
2134521RNAArabidopsis thaliana 345ucauugagug caucguugau g
2134621RNAArabidopsis thaliana 346uguguucuca ggucaccccu g
2134721RNAArabidopsis thaliana 347ugccaaagga gaguugcccu g
2134821RNAArabidopsis thaliana 348ugccaaagga gauuugcccc g
2134921RNAArabidopsis thaliana 349uuagauucac gcacaaacuc g
2135022RNAArabidopsis thaliana 350uuggggacga gauguuuugu ug
2235122RNAArabidopsis thaliana 351uuggggacga gauguuuugu ug
2235221RNAArabidopsis thaliana 352ccccuuacaa ugucgaguaa a
2135322RNADrosophila melanogaster 353uggaauguaa agaaguaugg ag
2235423RNADrosophila melanogaster 354uaauacuguc agguaaagau guc
2335517PRTHomo sapiens 355Met Glu Met Ala Leu Met Val Ala Gln Thr
Arg Lys Gly Lys Ser Val 1 5 10 15 Val 35653DNAHomo sapiens
356tggagatggc tctaatggtg gcacaaacca ggaaggggaa atctgtggtt taa
533571500DNAHomo sapiens 357gccgagcccg ggcccagcgc cgcctgcagc
ctcgggaagg gagcggatag cggagccccg 60agccgcccgc agagcaagcg cggggaacca
aggagacgct cctggcactg cagataactt 120gtctgcattt caagaacaac
ctaccagaga ccttacctgt caccttggct ctcccaccca 180atggagatgg
ctctaatggt ggcacaaacc aggaagggga aatctgtggt ttaaattctt
240tatgcctcat cctctgagtg ctgaaggctt gctgtaggct gtatgctgtt
aatgctaatc 300gtgatagggg tttttgcctc caactgactc ctacatatta
gcattaacag tgtatgatgc 360ctgttactag cattcacatg gaacaaattg
ctgccgtggg aggatgacaa agaagcatga 420gtcaccctgc tggataaact
tagacttcag gctttatcat ttttcaatct gttaatcata 480atctggtcac
tgggatgttc aaccttaaac taagttttga aagtaaggtt atttaaaaga
540tttatcagta gtatcctaaa tgcaaacatt ttcatttaaa tgtcaagccc
atgtttgttt 600ttatcattaa cagaaaatat attcatgtca ttcttaattg
caggttttgg cttgttcatt 660ataatgttca taaacacctt tgattcaact
gttagaaatg tgggctaaac acaaatttct 720ataatatttt tgtagttaaa
aattagaagg actactaacc tccagttata tcatggattg 780tctggcaacg
ttttttaaaa gatttagaaa ctggtacttt cccccaggta acgattttct
840gttcaggcaa cttcagttta aaattaatac ttttatttga ctcttaaagg
gaaactgaaa 900ggctatgaag ctgaattttt ttaatgaaat atttttaaca
gttagcaggg taaataacat 960ctgacagcta atgagatatt ttttccatac
aagataaaaa gatttaatca aaaaatttca 1020tatttgaaat gaagtcccaa
atctaggttc aagttcaata gcttagccac ataatacggt 1080tgtgcgagca
gagaatctac ctttccactt ctaagcctgt ttcttcctcc atatggggat
1140aatactttac aaggttgttg tgaggcttag atgagataga gaattattcc
ataagataat 1200caagtgctac attaatgtta tagttagatt aatccaagaa
ctagtcaccc tactttatta 1260gagaagagaa aagctaatga tttgatttgc
agaatattta aggtttggat ttctatgcag 1320tttttctaaa taaccatcac
ttacaaatat gtaaccaaac gtaattgtta gtatatttaa 1380tgtaaacttg
ttttaacaac tcttctcaac attttgtcca ggttattcac tgtaaccaaa
1440taaatctcat gagtctttag ttgatttaaa ataaaaaaaa aaaaaaaaaa
aaaaaaaaaa 150035823RNAHomo sapiens 358uaauacuguc agguaaagau guc
23359337DNAMedicago truncatula 359ttagggtttt cagctcatgg taataaaaat
gtcatctaat gtcttgcatg tgggaatgag 60gtcatatatg cagccaagga tgacttgccg
gcgagcctct ttcgatactt ttatgacata 120attaatcatg tggatagcca
aggtactaaa ctcactttgc actaaaacaa atatttttgc 180tttagtgcaa
acttagttta ggcgcttcgc aacggctagt caaatgtcct agttccaatg
240tgattggttg tccggcaagt cgtctctggc tacgtaaagg cctccttttt
tcatgctaga 300tttttgatga tttgatatag ccacacatat tttggaa
337360121DNAMedicago truncatula 360aagaggcaga gagagtaatg cagccaagga
tgacttgccg acaacattgg cgaatgttca 60tgtgatttct gcctcattgt gccggcaagt
tgtccttggc tatgttagtc tctcatcttc 120t 121361114DNAMedicago
truncatula 361tgaattcccc tccgcttttt gatgttggct tgtctcaatc
aaatcaaagt tcttgaaatt 60tgagttcttt agtctgattg agtcgtgcca atatcatatt
aagcgataaa agtc 114362367DNAMedicago truncatula 362ccacaaaact
ataactagct agaagcttta atcgccttat ttattataat aataataata 60aatatggctt
cagctgcaaa agtatacatg gcgtgatatt gatccggctc atctatatct
120tcaagttcaa tcatccatat tcatatcaat ttcagacgag ccgaatcaat
atcactcttg 180tttgcttcat tgcatattaa ttatatactt catttataag
ttatagtttg ccatatatat 240attagattga ttctgcagaa gtagacagga
gtggtgttgt ttctgctcat cttattaaat 300aatgaatgaa tgaatgacat
ttgcttactt ataagacgag ccgaatcaat atcactccag 360tacacct
367363135DNAMedicago truncatula 363aactgcaact tgaggaggca tccaaaggga
tcgcattgat cctataatat ttcaacttta 60gtcactttaa ttttctctca tataatactt
aattgggatc atgccatccc tttggatttc 120tcctttagta gctac
135364119DNAMedicago truncatula 364aggcatccaa agggatcgca ttgatcccaa
atctaattaa gtccctagct acttaattaa 60caacttaatt tccttaatat ctcataatat
ttgggatcat gctatccctt tggattcat 11936593DNAMedicago truncatula
365tgcttttcca cagctttctt gaacttcttt cgtatcttaa atctgttttc
aagattaaaa 60gtcctagaag ctcaagaaag ctgtgggaga ata
9336695DNAMedicago truncatula 366tattcttcca cagctttctt gaactgcatc
caaattgagt tcctttgcat tgccatggcc 60attgttttgc ggttcaataa agctgtggga
agata 9536721RNAMedicago truncatula 367cagccaagga ugacuugccg g
2136821RNAMedicago truncatula 368cagccaagga ugacuugccg a
2136921RNAMedicago truncatula 369ugauugaguc gugccaauau c
2137020RNAMedicago truncatula 370gagccgaauc aauaucacuc
2037121RNAMedicago truncatula 371uccaaaggga ucgcauugau c
2137221RNAMedicago truncatula 372uccaaaggga ucgcauugau c
2137321RNAMedicago truncatula 373uuccacagcu uucuugaacu u
2137421RNAMedicago truncatula 374uuccacagcu uucuugaacu g
2137515PRTArabidopsis thaliana 375Met Met Leu His Ile Thr His Arg
Phe Glu Ser Asp Val Gly Cys 1 5 10 15 3764PRTArabidopsis thaliana
376Met Leu Tyr Val 1 37714PRTArabidopsis thaliana 377Met Phe Met
Arg Arg Gly Leu Val Tyr Asn Asn Ile Tyr Ile 1 5 10
37817PRTArabidopsis thaliana 378Met Met Lys Val Cys Asp Glu Gln Asp
Gly Glu Ala Gly His Val His 1 5 10 15 Tyr 37928PRTArabidopsis
thaliana 379Met Lys Lys Arg Ile Thr Arg Ile Asn Leu Glu Glu Gln Ile
Lys Lys 1 5 10 15 Thr Leu Asp Asp Ser Arg Thr Arg Leu His Ser Pro
20 25 38010PRTArabidopsis thaliana 380Met Lys Lys Ile Gly Ser Ile
Asp Ser Phe 1 5 10 3816PRTArabidopsis thaliana 381Met Thr Cys Arg
Phe Lys 1 5 38211PRTArabidopsis thaliana 382Met Lys Asn Glu Asn Leu
Cys Gly Ser Gln Gly 1 5 10 38345PRTArabidopsis thaliana 383Met Lys
Cys Met Met Lys Lys Arg Gly Leu Thr Trp Arg Lys Ala Ser 1 5 10 15
Cys Leu Val Ala Lys Asp Asp Leu Pro Asp Leu Phe Arg Leu His Asp 20
25 30 Ser Ile Ser Asn Ser Cys Ile Leu Asp Tyr Tyr Thr Phe 35 40 45
3847PRTArabidopsis thaliana 384Met Phe Pro Arg Glu Ser Leu 1 5
38529PRTArabidopsis thaliana 385Met Thr Leu Ser Val Phe Phe His Ser
Phe Leu Glu Leu Gln Asn Phe 1 5 10 15 Phe Arg Phe Phe Phe Phe Ser
Phe Asp Ile Ser Tyr Ala 20 25 38623PRTArabidopsis thaliana 386Met
Ser Leu Ala Lys Gly Glu Leu Pro Cys His Cys Phe Arg Leu Asn 1 5 10
15 Thr Val Tyr Asn Arg Phe Cys 20 38748DNAArabidopsis thaliana
387atgatgttgc atatcacaca taggtttgag agtgatgttg gttgttga
4838815DNAArabidopsis thaliana 388atgctgtatg tatag
1538945DNAArabidopsis thaliana 389atgttcatgc gtagaggttt ggtatacaac
aatatataca tataa 4539054DNAArabidopsis thaliana 390atgatgaagg
tgtgtgatga gcaagatgga gaagcagggc acgtgcatta ctag
5439187DNAArabidopsis thaliana 391atgaagaaga gaatcactcg aattaatttg
gaagaacaaa ttaagaaaac cctagatgat 60tctcggacca ggcttcattc cccctaa
8739233DNAArabidopsis thaliana 392atgaagaaga tcggtagtat tgattcattt
taa 3339321DNAArabidopsis thaliana 393atgacttgcc gatttaaatg a
2139412DNAArabidopsis thaliana 394atggtgacat ga
1239536DNAArabidopsis thaliana 395atgaagaatg agaacttgtg tggtagccaa
ggatga 36396138DNAArabidopsis thaliana 396atgaagtgta tgatgaagaa
gagaggtcta acatggcgga aagcgtcatg tttagtagcc 60aaggatgact tgcctgatct
ttttcgcctc cacgattcaa tttcaaattc atgcattttg 120gattattata ccttttaa
13839724DNAArabidopsis thaliana 397atgtttccga gagagtccct ctga
2439890DNAArabidopsis thaliana 398atgaccctct ctgtattctt ccacagcttt
cttgaactgc aaaacttctt cagatttttt 60tttttttctt ttgatatctc ttacgcataa
9039972DNAArabidopsis thaliana 399atgtcacttg ccaaaggaga gttgccctgt
cactgcttcc gcttaaacac agtctataac 60cggttctgct aa
72400331DNAArabidopsis thaliana 400ctttgtcact tcatacactc cctattgtct
atatatatat atatacttac acatattcaa 60acattataat acttaattac acatacatac
tttatgatgt tgcatatcac acataggttt 120gagagtgatg ttggttgttg
acagaagata gagagcacta aggatgacat gcaagtacat 180acatatatat
catcacaccg catgtggatg ataaaatatg tataacaaat tcaaagaaag
240agagggagag aaagagagag aacctgcatc tctactcttt tgtgctctct
atacttctgt 300caccaccttt atctcttctt ctctctaacc t
331401304DNAArabidopsis thaliana 401atttactctt caccgccctc
tctctatata tagtctctat cctcacatat tatatatcaa 60accgcaagaa tgctgtatgt
atagtggagg gtgatagtgt ggttgctgac agaagataga 120gagcactaag
gatgctatgc aaaacagaca cagatatgtg tttctaattg tatttcatac
180tttaacctca aagttgatat aaaaaaagaa agaaagatag aagagctaga
agactatctg 240catctctatt cctatgtgct ctctatgctt ctgtcatcac
ctttctttct ctatttctct 300ctac 304402245DNAArabidopsis thaliana
402aaccaaaact cttcaacatt tctctctgac tacttcattt cctcttccca
acagttaaaa 60aaagttctga ttcgattcaa gccaagatcc acgtataaag atatgttcat
gcgtagaggt 120ttggtataca acaatatata catataatag tttgtcgtta
tgcctggctc cctgtatgcc 180acgagtggat accgattttg gttttaaaat
cggctgccgg tggcgtacaa ggagtcaagc 240atgac 245403240DNAArabidopsis
thaliana 403atacattctc tctttctctc tctctctctc tctcatcccg gcccagttat
gtggtcggag 60agaatgatga aggtgtgtga tgagcaagat ggagaagcag ggcacgtgca
ttactagctc 120atatatacac tctcaccaca aatgcgtgta tatatgcgga
attttgtgat atagatgtgt 180gtgtgtgttg agtgtgatga tatggatgag
ttagttcttc atgtgcccat cttcaccatc 240404280DNAArabidopsis thaliana
404tcacacatac ctttctttct cttcttcttc ttacgaaaag tttcatcaca
ttcacattat 60ctttaacttt ggtctctttt cttttttgtc tcttttctct tcttgataac
gtggttctag 120tcttgattaa ttcattgttg tgcgatttag tgttgagagg
attgttgtct ggctcgaggt 180catgaagaag agaatcactc gaattaattt
ggaagaacaa attaagaaaa ccctagatga 240ttctcggacc aggcttcatt
ccccctaacc tacttatcgc 280405179DNAArabidopsis thaliana
405atttagcttc ttcttcttct tcttcttctg tctacttaca taaagttatc
cttgctttgg 60tttagggttg agaggaatat tgtctggctc gaggtcatga agaagatcgg
tagtattgat 120tcattttaaa gagtgaaatc cctaaatgat tctcggacca
ggcttcattc cccccaacc 179406376DNAArabidopsis thaliana 406tagtattcat
aagcaccaaa acaaatatgt agagatctcc tcttccattc tctattgtta 60ctttcgagaa
gaaacataca aaacacatac atttttcttt tgtttgtggt tttcatatat
120acaagtgggt atagctagtg aaacgcgaat gtgacgaaag tagtgtgcag
ccaaggatga 180cttgccgatt taaatgatct ttctttatac tctattaaga
caatttagtt tcaaactttt 240tttttttttt ttttttgaag gattcaggaa
gaaattagga tatattattc cgtataaaat 300acaagatata taaaaccaaa
aagaaaaagt aacatgatcg gcaagttgtc cttggctaca 360cgttactttg tgtcgc
37640777DNAArabidopsis thaliana 407actcatcaac aacctcttca taaatacata
aatcatataa gagaaaatgg tgacatgaag 60aatgagaact tgtgtgg
77408248DNAArabidopsis thaliana 408aggcaaaaac atatagagag taatgaagtg
tatgatgaag aagagaggtc taacatggcg 60gaaagcgtca tgtttagtag ccaaggatga
cttgcctgat ctttttcgcc tccacgattc 120aatttcaaat tcatgcattt
tggattatta taccttttaa agtataatag gtcaaatatc 180atgttgaatc
ttgcgggtta ggtttcaggc agtctctttg gctatcttga catgcttttt 240ccatccat
248409187DNAArabidopsis thaliana 409attcactccc ttcttcttct
taatctcctt acagttacag acattctctc acttgcgttc 60ttgtttcttt tacaaaacag
atacactatg tttccgagag agtccctctg atattggcct 120ggttcactca
gattctcttt tactaactca tctgattgag ccgtgtcaat atctcagtcc 180tctctcg
187410246DNAArabidopsis thaliana 410tctcacaact tcaacttccc
tctttctcta tattacgctt ttgcccctca ctccctcttt 60ccacaattag ggtttcgtct
gctctacatg accctctctg tattcttcca cagctttctt 120gaactgcaaa
acttcttcag attttttttt ttttcttttg atatctctta cgcataaaat
180agtgattttc ttcatatctc tgctcgattg atttgcggtt caataaagct
gtgggaagat 240acagac 246411631DNAArabidopsis thaliana 411gaataaccaa
ccagccttct ctcaaagcaa accaaaaaga aaaaccaaca ttgaaagagg 60aagttacgat
aagcggagca gtaatagggc atctttctat tggcaggcga cttggctatt
120tgtatctttt gtgttcttga ctattggcta tgtcacttgc caaaggagag
ttgccctgtc 180actgcttccg cttaaacaca gtctataacc ggttctgcta
atatcaatcc ttcttttgga 240catgtccaaa gccgagattg attgatagag
aattggtctc tctggctaca aaactagtgc 300ggttctctcg atttaagttt
taatagcatt cactttgcac attgcatctt tcacatcaaa 360tttccatttc
atcaaccatc taaacctctt tgttagcttt gatataagca acgatctaaa
420gtctaaaaac cattaatcct ctgaaaaaaa agacaatttc gatggttcta
ttatgtttct 480ccaatgcaga aattgtatcg tctgaattat agtagatttt
ttctagacta aagtgtaaac 540caagacgaat ctgcactaac aagacacacc
aatagacttt acagagaaag gttacgagtt 600ttgaaaatat taacggacca
tagtcatcgc g 63141221RNAArabidopsis thaliana 412uugacagaag
auagagagca c 2141320RNAArabidopsis thaliana 413ugacagaaga
uagagagcac 2041421RNAArabidopsis thaliana 414ugccuggcuc ccuguaugcc
a 2141521RNAArabidopsis thaliana 415uggagaagca gggcacgugc a
2141621RNAArabidopsis thaliana 416ucggaccagg cuucauuccc c
2141721RNAArabidopsis thaliana 417ucggaccagg cuucauuccc c
2141821RNAArabidopsis thaliana 418cagccaagga ugacuugccg a
2141921RNAArabidopsis thaliana 419uagccaagga ugacuugccu g
2142021RNAArabidopsis thaliana 420uagccaagga ugacuugccu g
2142121RNAArabidopsis thaliana 421ugauugagcc gugucaauau c
2142221RNAArabidopsis thaliana 422uuccacagcu uucuugaacu g
2142321RNAArabidopsis thaliana 423ugccaaagga gaguugcccu g
2142413PRTMedicago truncatula 424Met Val Lys Glu Ser Phe Met Glu
Arg Leu Lys Val Arg 1 5 10 42542DNAMedicago truncatula
425atggtcaaag agtctttcat ggagaggttg aaagtgagat ga
42426291DNAMedicago truncatula 426tggataagtt aatttatggt caaagagtct
ttcatggaga ggttgaaagt gagatgaagc 60caaggatgac ttgccggtat aatagtaatt
tgccacaaat ctagatagct attagctatg 120tttggatggg cggtgagatt
aacaaaatta cagcagcatt gtgattttgt tgatgcttta 180aagtgtagtt
tttatcaaaa ttacagtggt tcactgtaat tatgagaatc tcaccgtcaa
240tctaaatatg catttagttt catttccggc aggtcatcct tcggctatat t
29142721RNAMedicago truncatula 427aagccaagga ugacuugccg g
2142820PRTArtificial SequencePrimer 428Leu Ile Val Ser His Leu Tyr
Ser Glu Lys Phe Asp Cys Met Arg Lys 1 5 10 15 Ile Leu Arg Ile 20
42920DNAArtificial SequencePrimer 429tccccaaatt cttgaccaaa
2043020DNAArtificial SequencePrimer 430ttgaggggaa aacatgaacc
2043120DNAArtificial SequencePrimer 431acgaaatccg tctcatttgc
2043220DNAArtificial SequencePrimer 432gggtgaagag ctcatgttgg
2043319DNAArtificial SequencePrimer 433tgaggggaat gttgtctgg
1943419DNAArtificial SequencePrimer 434gaagcctggt ccgaggata
1943520DNAArtificial SequencePrimer 435cgagtcgcca aaattcaaac
2043620DNAArtificial SequencePrimer 436gctcccttca gtccaatcaa
2043720DNAArtificial SequencePrimer 437gcaactgcag tggaatagca
2043820DNAArtificial SequencePrimer 438gcgaccttca tgggttctaa
2043920DNAArtificial SequencePrimer 439ctcagcatca gcaacgtgat
2044020DNAArtificial SequencePrimer 440aactctgcta gggcctcctc
2044120DNAArtificial SequencePrimer 441tcacaactcc tcagcattcg
2044220DNAArtificial SequencePrimer 442acccaatcaa cagcagttcc
2044320DNAArtificial SequencePrimer 443ggtaacattg tgctcagtgg
2044420DNAArtificial SequencePrimer 444ctcggccttg gagatccaca
2044530DNAArtificial SequencePrimer 445tcatcgatga atatacatac
ataccatcat 3044633DNAArtificial SequencePrimer 446tccggatcct
ctaaaagcta aagtgattct aaa 3344726DNAArtificial SequencePrimer
447tcatcgagag agagcttcct tgagtc 2644828DNAArtificial SequencePrimer
448tccggatcca gagggagctc ccttcagt 2844926DNAArtificial
SequencePrimer 449tcatcggttg aggggaatgt tgtctg 2645027DNAArtificial
SequencePrimer 450tccggatccg ttggggggga tgaagcc
2745128DNAArtificial SequencePrimer 451tcatcgatga gggttaagct
atttcagt 2845231DNAArtificial SequencePrimer 452tccggatcct
aatatcctcg atccagacaa c 3145321DNAArtificial SequencePrimer
453cgcctcaatt tgaatacatg g 2145420DNAArtificial SequencePrimer
454acgcggctca atcaaactac 2045523DNAArtificial SequencePrimer
455gcactagctg gtttcattat tcc 2345621DNAArtificial SequencePrimer
456ttgcaaaatt tggagagcct a 2145720DNAArtificial SequencePrimer
457aagcgatgtt ggtgaggttc 2045821DNAArtificial SequencePrimer
458cggctcaatc tgagatcgta t 2145920DNAArtificial SequencePrimer
459gggcggtgag attaacaaaa 2046020DNAArtificial SequencePrimer
460cctgccggaa atgaaactaa 2046126DNAArtificial SequencePrimer
461tcatcgatga tggtgtttgg gaagcc 2646230DNAArtificial SequencePrimer
462tccggatcct acatgtaaat ccgtcttcgg 3046321DNAArtificial
SequencePrimer 463aggcttcttc ataggctctc c 2146439DNAArtificial
SequencePrimer 464tcagtccgct cgaggaataa gtgaatatta tcgatattt
3946538DNAArtificial SequencePrimer 465aaggaaaaaa gcggccgcaa
agtgatattg gcgcggct 3846667DNAArtificial SequencePrimer
466tagcatgtta ttacaccgct taagtaagtt ctgtaagata gagcgcgata
ttgtttacat 60tagctag 6746767DNAArtificial SequencePrimer
467cgtactagct aatgtaaaca atatcgcgct ctatcttaca gaacttactt
aagcggtgta 60ataacat 6746838DNAArtificial SequencePrimer
468aaaaggtctc gccataagca tagtggaata atgaaacc 3846937DNAArtificial
SequencePrimer 469aaaaggtctc gccatttcca tacctgaatt tctcctt
3747038DNAArtificial SequencePrimer 470aaaaggtctc gccatcttca
tcctcaatat cctaattc 3847144DNAArtificial SequencePrimer
471aggtctcgac acagatatat atactatgtc tctttcaatt ttgc
4447235DNAArtificial SequencePrimer 472aaaggtctcg ccatgaccat
tttcccacga atatg 3547336DNAArtificial SequencePrimer 473aaggtctcga
cacaagcata gtggaataat gaaacc 3647438DNAArtificial SequencePrimer
474aaggtctcga cacagatata tatactatgt ctctttca 3847522DNAArtificial
SequencePrimer 475gaattcctgc ccatggtttt cg 2247622DNAArtificial
SequencePrimer 476cgaaaaccat gggcaggaat tc 2247723DNAArtificial
SequencePrimer 477cagtcccgac ttaagctcaa tac 2347823DNAArtificial
SequencePrimer 478gtattgagct taagtcggga ctg 2347943DNAArtificial
SequencePrimer 479aaaggtctcc tagcttggtc aaacatacat acagtagcac tag
4348046DNAArtificial SequencePrimer 480aaaggtctcg cgtaaaattg
tactgattga atgtaaattg gtacac 4648122DNAArtificial SequencePrimer
481tagcatgttt tcccctcaat ga 2248222DNAArtificial SequencePrimer
482cgtatcattg aggggaaaac at 2248336DNAArtificial SequencePrimer
483aaaggtctcc tagcactcat aactctcccc aaattc 3648437DNAArtificial
SequencePrimer 484aaaggtctcg cgtagaaaga atgttcgaaa aacaatg
3748535DNAArtificial SequencePrimer 485aggtctccaa atcatgaagc
aggcagtaat aacct 3548664DNAArtificial SequencePrimer 486aggtctcgcg
tactaagcgt aatctggaac atcgtatggg tataatatcc tcgatccaga 60caac
6448731DNAArtificial SequencePrimer 487aggtctcgcc atagtggcgg
agacgaagat g 3148834DNAArtificial SequencePrimer 488aggtctcgcc
attaatatcc tcgatccaga caac 3448934DNAArtificial SequencePrimer
489aggtctccaa attaaactgt cagtgcatgg atgt 3449037DNAArtificial
SequencePrimer 490aggtctcgac acattcacaa atttttgttg tagagag
3749137DNAArtificial SequencePrimer 491aggtctcgac acattaacaa
atttttgttg tagagag 3749236DNAArtificial SequencePrimer
492aggtctcgac actagcagat tcacaaattt ttgttg 3649335DNAArtificial
SequencePrimer 493aggtctcgac accctcatga taatcgatct tagca
35494272DNADrosophila melanogaster 494cgcacacaca cacacacaca
atcactcaca cgcggtcaca cgcacatttc aataaactaa 60tggagcctgg ctttgttttt
gttttatttc caacccactt gagcacacag cacacacaga 120gagaaaaatc
aatactcgtt atgggattaa atttacaaag cgcaaagcaa agcgacaaac
180aaaattcaaa agaaagaaaa aaaaacactc aaataaactc acaaagaatt
ccttatcgcc 240aagggggcca atgttctaag gttctttcgc ct
272495272DNAArtificial sequenceMutated sequence 495cgcacacaca
cacacacaca atcactcaca cgcggtcaca cgcacatttc aataaactaa 60gtgagcctgg
ctttgttttt gttttatttc caacccactt aagcacacag cacacacaga
120gagaaaaatc aatactcgtt agtggattaa atttacaaag cgcaaagcaa
agcgacaaac 180aaaattcaaa agaaagaaaa aaaaacactc aaataaactc
acaaagaatt ccttatcgcc 240aagggggcca atgttctaag gttctttcgc ct
272
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