U.S. patent application number 15/074330 was filed with the patent office on 2016-07-07 for genetically modified microorganisms capable of producing beta-glucans and methods for producing beta-glucans.
The applicant listed for this patent is BASF SE, Wintershall Holding GmbH. Invention is credited to Beata Brockmann, Christian Fleck, Mari Granstrom, Stefan Haefner, Andrea Herold, Julia Kristiane Schmidt, Hartwig Schroder, Oskar Zelder.
Application Number | 20160194676 15/074330 |
Document ID | / |
Family ID | 49878797 |
Filed Date | 2016-07-07 |
United States Patent
Application |
20160194676 |
Kind Code |
A1 |
Brockmann; Beata ; et
al. |
July 7, 2016 |
GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING
BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS
Abstract
The present invention relates to genetically modified
microorganisms capable of producing beta-glucans, characterized in
that the genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain. The present
invention also relates to the use of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity or the use
of such a polypeptide for producing .beta.-glucans. Furthermore,
the present invention relates to methods for producing
.beta.-glucans comprising the introduction of a promoter upstream
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity thereby increasing the
expression of the polynucleotide, or a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism being able to synthesize .beta.-glucans.
Inventors: |
Brockmann; Beata;
(Morristown, NJ) ; Herold; Andrea; (Weinheim,
DE) ; Zelder; Oskar; (Speyer, DE) ; Haefner;
Stefan; (Speyer, DE) ; Fleck; Christian;
(Sandhausen, DE) ; Schroder; Hartwig; (Nu loch,
DE) ; Granstrom; Mari; (Kerava, FI) ; Schmidt;
Julia Kristiane; (Heidelberg, DE) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
BASF SE
Wintershall Holding GmbH |
Ludwigshafen
Kassel |
|
DE
DE |
|
|
Family ID: |
49878797 |
Appl. No.: |
15/074330 |
Filed: |
March 18, 2016 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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13935043 |
Jul 3, 2013 |
9322041 |
|
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15074330 |
|
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61667961 |
Jul 4, 2012 |
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Current U.S.
Class: |
435/97 ;
435/254.11 |
Current CPC
Class: |
C12N 9/1051 20130101;
C12P 19/18 20130101; C12P 19/04 20130101; Y02P 20/52 20151101; C08B
37/0024 20130101; C12N 15/80 20130101; C12Y 204/01034 20130101;
C08L 5/00 20130101 |
International
Class: |
C12P 19/04 20060101
C12P019/04; C12N 9/10 20060101 C12N009/10; C12P 19/18 20060101
C12P019/18 |
Claims
1. A genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase activity, wherein said polynucleotide comprises a
nucleotide sequence having at least 70% sequence identity to the
nucleotide sequence of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO: 11,
or SEQ ID NO: 15, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase activity, wherein said polypeptide
comprises an amino acid sequence having at least 70% sequence
identity to the amino acid sequence of SEQ ID NO: 8 or SEQ ID NO:
16, compared to a corresponding non-modified control microorganism
of the same strain.
2. The genetically modified microorganism of claim 1, wherein said
polymer is selected from the group consisting of schizophyllan,
scleroglucan, pendulan, cinerian, laminarin, lentinan and
pleuran.
3. The genetically modified microorganism of claim 1, wherein said
polynucleotide is a 1,3-.beta.-D-glucan synthase gene.
4. The genetically modified microorganism of claim 1, wherein said
polynucleotide comprises a nucleotide sequence having at least 80%
sequence identity to the nucleotide sequence of SEQ ID NO: 3, SEQ
ID NO: 7, SEQ ID NO: 11, or SEQ ID NO: 15.
5. The genetically modified microorganism of claim 1, wherein said
polynucleotide comprises a nucleotide sequence having at least 90%
sequence identity to the nucleotide sequence of SEQ ID NO: 3, SEQ
ID NO: 7, SEQ ID NO: 11, or SEQ ID NO: 15.
6. The genetically modified microorganism of claim 1, wherein said
polypeptide is a 1,3-.beta.-D-glucan synthase.
7. The genetically modified microorganism of claim 1, wherein said
polypeptide comprises an amino acid sequence having at least 80%
sequence identity to the amino acid sequence of SEQ ID NO: 8 or SEQ
ID NO: 16.
8. The genetically modified microorganism of claim 1, wherein said
polypeptide comprises an amino acid sequence having at least 90%
sequence identity to the amino acid sequence of SEQ ID NO: 8 or SEQ
ID NO: 16.
9. The genetically modified microorganism of claim 1, wherein said
polynucleotide comprises the nucleotide sequence of SEQ ID NO: 3,
SEQ ID NO: 7, SEQ ID NO: 11, or SEQ ID NO: 15, or wherein said
polynucleotide encodes a polypeptide comprising the amino acid
sequence of SEQ ID NO: 4, SEQ ID NO: 8, SEQ ID NO: 12, or SEQ ID
NO: 16.
10. The genetically modified microorganism of claim 1, wherein said
microorganism is selected from the group consisting of
Schizophyllum commune, Sclerotium rolfsii, Sclerotium glucanicum,
Sclerotium delphinii, Porodisculus pendulus, Botrytis cinerea,
Laminaria sp., Lentinula edoles, and Monilinia fructigena.
11. The genetically modified microorganism of claim 1, wherein said
modified microorganism is able to produce at least 1.5 times more
of said polymer compared to said non-modified control
microorganism.
12. A method of producing a polymer consisting of a linear main
chain of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
being able to synthesize said polymer, wherein said polynucleotide
comprises: (i) a nucleotide sequence having at least 70% sequence
identity to the nucleotide sequence of SEQ ID NO: 3, SEQ ID NO: 7,
SEQ ID NO: 11, or SEQ ID NO: 15; or (ii) a nucleotide sequence
encoding a polypeptide having at least 70% sequence identity to the
amino acid sequence of SEQ ID NO: 8 or SEQ ID NO: 16, and wherein
said polynucleotide is optionally downstream of a strong promoter
thereby increasing the expression of said polynucleotide; (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce said polymer; and (c) optionally
recovering said polymer from the medium.
13. The method of claim 12, wherein said polymer is selected from
the group consisting of schizophyllan, scleroglucan, pendulan,
cinerian, laminarin, lentinan and pleuran.
14. The method of claim 12, wherein said polynucleotide is a
1,3-.beta.-D-glucan synthase gene.
15. The method of claim 12, wherein said polynucleotide comprises a
nucleotide sequence having at least 80% sequence identity to the
nucleotide sequence of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO: 11,
or SEQ ID NO: 15.
16. The method of claim 12, wherein said polynucleotide encodes a
1,3-.beta.-D-glucan synthase.
17. The method of claim 12, wherein said polynucleotide encodes a
polypeptide having at least 80% sequence identity to the amino acid
sequence of SEQ ID NO: 8 or SEQ ID NO: 16.
18. The method of claim 12, wherein said polynucleotide comprises:
(a) a nucleotide sequence having at least 90% sequence identity to
the nucleotide sequence of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO:
11, or SEQ ID NO: 15; or (b) a nucleotide sequence encoding a
polypeptide having at least 90% sequence identity to the amino acid
sequence of SEQ ID NO: 8 or SEQ ID NO: 16.
19. The method of claim 12, wherein said polynucleotide comprises
the nucleotide sequence of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO:
11, or SEQ ID NO: 15, or wherein said polynucleotide encodes a
polypeptide comprising the amino acid sequence of SEQ ID NO: 4, SEQ
ID NO: 8, SEQ ID NO: 12, or SEQ ID NO: 16.
20. The method of claim 12, wherein said microorganism is selected
from the group consisting of Schizophyllum commune, Sclerotium
rolfsii, Sclerotium glucanicum, Sclerotium delphinii, Porodisculus
pendulus, Botrytis cinerea, Laminaria sp., Lentinula edoles, and
Monilinia fructigena.
Description
RELATED APPLICATIONS
[0001] This application is a divisional of U.S. application Ser.
No. 13/935,043, filed Jul. 3, 2013, which claims benefit (under 35
USC 119(e)) of U.S. Provisional Application No. 61/667,961, filed
Jul. 4, 2012. The entire contents of each of these applications are
hereby incorporated by reference herein in their entirety.
SUBMISSION OF SEQUENCE LISTING
[0002] The Sequence Listing associated with this application is
filed in electronic format via EFS-Web and hereby incorporated by
reference into the specification in its entirety. The name of the
text file containing the Sequence Listing is
Sequence_Listing_074008_1544_01. The size of the text file is 185
KB, and the text file was created on Mar. 18, 2016.
[0003] The present invention relates to genetically modified
microorganisms capable of producing beta-glucans (herein also
referred to as .beta.-glucans), characterized said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain. D-glucan synthase-activity compared to a
corresponding non-modified control microorganism of the same
strain. The present invention also relates to the use of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity or the use of such a polypeptide for producing
.beta.-glucans. Furthermore, the present invention relates to
methods for producing .beta.-glucans comprising the introduction of
a polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity into a microorganism being able to synthesize
.beta.-glucans. In context of the present invention, the term
".beta.-glucans" may particularly comprise polymers consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3. .beta.-glucans are known
well-conserved components of cell walls in several microorganisms,
particularly in fungi and yeast (Novak, Endocrine, Metabol &
Immune Disorders--Drug Targets (2009), 9: 67-75). Biochemically,
.beta.-glucans comprise non-cellulosic polymers of .beta.-glucose
linked via glycosidic .beta.(1-3) bonds exhibiting a certain
branching pattern with .beta.(1-6) bound glucose molecules (Novak,
loc cit). A large number of closely related .beta.-glucans exhibit
a similar branching pattern such as schizophyllan, scleroglucan,
pendulan, cinerian, laminarin, lentinan and pleuran, all of which
exhibit a linear main chain of .beta.-D-(1-3)-glucopyranosyl units
with a single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3 (Novak, loc cit; EP-B1
463540; Stahmann, Appl Environ Microbiol (1992), 58: 3347-3354;
Kim, Biotechnol Letters (2006), 28: 439-446; Nikitina, Food Technol
Biotechnol (2007), 45: 230-237). Although these .beta.-glucans are
structurally closely related, their respective microbial producers
are not. Examples of microorganisms producing these structurally
closely related .beta.-glucans are Schizophyllum commune (for
schizophyllan; Martin, Biomacromolecules (2000), 1: 49-60; Rau,
Methods in Biotechnol (1999), 10: 43-55, DOI:
10.1007/978-1-59259-261-6_4); Sclerotium rolfsii, Sclerotium
glucanicum, and Sclerotium delphinii (for scleroglucan; Survase,
Food Technol Biotechnol (2007), 107-118); Porodisculus pendulus
(for pendulan; EP-B1 463540); Botrytis cinerea (for cinerian;
Stahmann, loc cit) Laminaria sp. (for laminarin; Kim, loc cit); and
Lentinula edoles (for lentinan, Nikitina, loc cit). At least two of
said .beta.-glucans-schizophyllan and scleroglucan--even share an
identical structure and differ only slightly in their molecular
mass, i.e. in their chain length (Survase, loc cit).
[0004] Such .beta.-glucans are widely used as thickeners and find
application in several applications such as food industry and
particularly oil industry (enhanced oil recovery, EOR) (Survase,
loc cit). Also, such .beta.-glucans are used in the pharmaceutical
industry in tablet formulations and excipients as well as in
immunotherapy as antiviral agents (Survase, loc cit).
[0005] Industrial production of .beta.-glucans is mostly performed
by fermentation processes using their natural microbial producers.
Classical ways to improve .beta.-glucan synthesis, e.g., of
schizophyllan is based on manipulation of the development of S.
commune (Rau, Habilitation, Braunschweig 1997). The most common
approach is to convert dicaryotic cells via protoplast generation
into monocarytic cells (Rau, Habilitation, Braunschweig 1997).
Another approach is to cross different monocaryotic cells to form a
new dicaryotic cell (Rau, Habilitation, Braunschweig 1997). Further
possible approaches comprise, e.g., a classical random based
mutagenesis using UV radiation, transposon mutagenesis or using
suitable chemicals (e.g., nitrosoguanidin (NTG or
N-methyl-N'-nitro-N-nitrosoguanidin), 2-aminofluorene (2-AF),
4-nitro-o-phenylenediamine (NPD),
2-methoxy-6-chloro-9-(3-(2-chloroethyl)
aminopropylamino)acridine.times.2HCl (ICR-191),
4-nitroquinolone-N-oxide (NQNO), benzo[.alpha.]pyrene (B[alpha]p),
or sodium azide (SA)) (Czyz, J Appl Genet (2002), 43(3): 377-389).
Due to the rearrangement of genetic material within the crossing
event it is possible to select strains exhibiting higher
.beta.-glucan (schizophyllan) productivity.
[0006] Yet, all of these approaches are undirected and do not allow
targeted modification of the .beta.-glucan producing
microorganisms. In fact, results and efficiency of such approaches
are not predictable and identification and selection of improved
strains is labored and costly.
[0007] This technical problem has been solved by the means and
methods described herein below and as defined in the claims.
[0008] In particular, as has been surprisingly found in context
with the present invention, overexpression of 1,3-.beta.-D-glucan
synthase in a .beta.-glucan producing microorganism such as, e.g.,
S. commune or S. rolfsii leads to significant higher yields of the
respective .beta.-glucan. This finding was indeed unexpected given
the fact that the biosynthetic pathway of .beta.-glucan synthesis
was only poorly understood and moreover, for most .beta.-glucan
producing microorganisms (such as Schizophyllum commune), there was
no proposed .beta.-glucan biosynthesis pathway available at all.
Moreover, in context of those microorganisms whose .beta.-glucan
biosynthesis pathway was at least investigated (such as Pediococcus
parvulus), enzymes such as .alpha.-phosphoglucomutase (.alpha.-PGM)
and particularly UDP-glucose pyrophosphorylase (UGP) were assumed
to represent a bottle-neck in .beta.-glucan synthesis (Velasco, Int
J Food Microbiol (2007), 115: 325-354). Accordingly, overexpression
of these enzymes was assumed to increase the yields of
.beta.-glucan synthesis (Velasco, loc cit). Yet, as has been found
in context with the present invention, overexpression of UGP in S.
commune did not result in an increased yield of the .beta.-glucan
schizophyllan. In sharp contrast, as further described herein below
and in the Examples, it has been found in context of the present
invention that S. commune possesses two copies of
1,3-.beta.-D-glucan synthase (genome sequence known from Ohm,
Nature Biotech (2010), 28: 957-963) and, surprisingly, that
overexpressing either of the two copies of 1,3-.beta.-D-glucan
synthase in S. commune leads to significant higher yields in the
production of schizophyllan. Given that schizophyllan has a
structure which is closely related to other .beta.-glucans such as
scleroglucan, pendulan, cinerian, laminarin, lentinan and pleuran
(all of which are polymers consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3), it appears to be likely
that overexpression of polypeptides having 1,3-.beta.-D-glucan
synthase activity in corresponding microorganisms as also described
herein may therefore result in higher yields of those
.beta.-glucans.
[0009] Accordingly, the present invention relates to a genetically
modified microorganism capable of producing a polymer consisting of
a linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain. Said
polynucleotide may be endogenous or exogenous. For example, in
context with the present invention, the overexpression of said
polynucleotide may result from the introduction of a strong (e.g.,
constitutive or inducible) promoter upstream of said polynucleotide
thereby increasing the expression level of said polynucleotide, or,
preferably, from the introduction of at least one copy of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity. In one embodiment, the present invention relates
to a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain. Said
genetically modified microorganism is preferably capable of stably
maintaining and expressing the additional polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity. Said
genetically modified microorganism may originate from a
corresponding non-modified microorganism which preferably per se,
i.e. naturally, contains a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity. Also, said
genetically modified microorganism is preferably per se, i.e.
before modification, able to produce a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3 as described herein. Into
said genetically modified microorganism, a strong promoter or at
least one polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity may have been introduced.
Non-limiting examples of means and methods for the introduction of
a promoter sequence into a microorganism may comprise inter alia
homologous recombination as known in the art (Ohm, World J
Microbiol Biotechnol (2010), 26: 1919-1923). Also, in context with
the present invention, the microorganism may have been modified
such that more polypeptide having 1,3-.beta.-D-glucan
synthase-activity is expressed, e.g., by inserting a strong
promoter as described herein, by adding introns into a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, by adapting the codon usage, by improving the
ribosomal binding site for better translational initiation, by
introducing elements in the mRNA that stabilize it, or by inserting
a polynucleotide with a higher transcription level having
1,3-.beta.-D-glucan synthase-activity into the microorganism (cf.
Ohm, loc cit).
[0010] In context with the present invention, the promoter may be
introduced into said microorganism upstream of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
and in a manner that said promoter increases or enhances the
expression of said polynucleotide. Non-limiting examples of means
and methods for the introduction of a polynucleotide into a
microorganism may comprise transformation, transduction and
transfection as commonly known in the art and as also exemplified
herein (Sambrook and Russell (2001), Molecular Cloning: A
Laboratory Manual, CSH Press, Cold Spring Harbor, N.Y., USA;
Current Protocols in Molecular Biology, Update May 9, 2012, Print
ISSN: 1934-3639, Online ISSN: 1934-3647; Methods in Yeast Genetics,
A Laboratory Course Manual, Cold Spring Harbor Laboratory Press,
1990; van Peer, Applied Environ Microbiol (2009), 75: 1243-1247;
Schmid, "Genetics of Scleroglucan Production by Sclerotium
rolfsii", dissertation Technische Universitat Berlin, D83 (2008)).
Non-limiting examples of means and methods for the introduction of
a promoter sequence into a microorganism may comprise inter alia
homologous recombination as known in the art (Ohm, World J
Microbiol Biotechnol (2010), 26: 1919-1923). Strong promoters to be
introduced upstream of a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity in context with the
present invention may comprise, inter alia, constitutive promoters
such as, e.g., tef1 promoter (translation and elongation factor 1a,
S. commune, A. niger), gpdA promoter (glyceraldehyde-3-phosphate
dehydrogenase, S. commune, A. niger, Schuren, Cur Genet (1998), 33:
151-156), trpC promoter (tryptophan biosynthesis, Aspergilus
nidulans) or inducible promoters such as, e.g., glaA promoter
(glucoamylase, A. niger), alcA (alcohol dehydrogenase, A. nidulans)
cbhl (cellobiohydrolase I, Trichoderma reesei; Knabe, Dissertation
"Untersuchung von Signalkomponenten der sexuellen Entwicklung bei
dem Basidiomyceten Schizophyllum commune" (2008)) thiA (thiamine
biosynthesis, Aspergillus oryzae) (Moore, Biotechnology, Vol. III,
Genetic Engineering of Fungal Cells, Enceclopedia of Life Support
Systems (2007)). In context with the present invention, preferred
promoters comprise tef1 and gdpA.
[0011] Generally, in context with the present invention, the
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity may be introduced into the microorganism in any
suitable form, e.g., comprised in a vector, a plasmid, or as naked
nucleic acid as further described and exemplified herein. The
polynucleotide introduced into the microorganism may then be
exogenous, on a vector/plasmid within the microorganism (i.e.
outside of the microbial chromosome(s)), or it may be incorporated
into the microbial chromosome(s) by, e.g., random (ectopic) or
homologous recombination or any other suitable method as known in
the art. In context with the present invention, the polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
which has been introduced into the microorganism (i.e. the
additional copy to the natural endogenous polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity of a
corresponding unmodified strain) does not necessarily have to have
the same nucleotide sequence as the natural endogenous
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity of a corresponding unmodified strain, as long as
it has 1,3-.beta.-D-glucan synthase-activity as described
herein.
[0012] In one embodiment of the present invention, the genetically
modified microorganism is able to produce at least 1.5 times, more
preferably at least 1.8 times more, more preferably at least 2.0
times more, and most preferably at least 2.2 times more
.beta.-glucan polymer compared to the corresponding non-modified
control microorganism. In this context, production of, e.g., 1.5
times "more" .beta.-glucan polymer may mean that a genetically
modified microorganism produces an amount of .beta.-glucan polymer
which is 1.5 times higher compared to the amount of .beta.-glucan
polymer produced in the same time under the same conditions by a
corresponding non-modified control microorganism. Alternatively,
production of, e.g., 1.5 times "more" .beta.-glucan polymer may
mean that a genetically modified microorganism produces the same
amount of .beta.-glucan polymer as a corresponding non-modified
control organism under the same conditions, however, 1.5 times
faster. The amount of produced .beta.-glucan polymer may be
measured by methods known in the art and as also described
herein.
[0013] Furthermore, the present invention relates to the use of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, or a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, or of a genetically modified microorganism
according to claim 1 for producing a polymer consisting of a linear
main chain of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0014] Furthermore, the present invention relates to a method of
producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0015] (a) introducing (i) a strong (e.g., constitutive
or inducible) promoter upstream of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity thereby
increasing the expression of said polynucleotide, or, preferably,
(ii) a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity into a microorganism being
able to synthesize said polymer; [0016] (b) culturing said
microorganism of (a) in a medium, thereby allowing said
microorganism to produce said polymer; and [0017] (c) optionally
recovering said polymer from the medium.
[0018] As regards step (c) of the method described and provided
herein, it is noted that in some cases (e.g., when .beta.-glucans
such as schizophyllan is used for oil drilling purposes), the
culture broth may also be used directly (e.g., pumped into the
drill hole), without previous recovery of the pure .beta.-glucan.
As such, the recovery step (c) is optional. Strong promoters to be
introduced upstream of a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity in context with the
present invention may comprise, inter alia, constitutive promoters
such as, e.g., tef1 promoter (translation and elongation factor 1a,
S. commune, A. niger), gpdA promoter (glyceraldehyde-3-phosphate,
S. commune, A. niger), trpC promoter (tryptophan biosynthesis,
Aspergillus nidulans) or inducible promoters such as, e.g., glaA
promoter (glucoamylase, A. niger), alcA (alcohol dehydrogenase, A.
nidulans) cbhl (cellobiohydrolase I, Trichoderma reesei) thiA
(thiamine biosynthesis, Aspergillus oryzae), tef1 and gdpA being
preferred promoters. In context with the present invention, the
promoter is preferably introduced into said microorganism upstream
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity and in a manner that said
promoter increases or enhances the expression of said
polynucleotide. Said promoter or said polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity may be
introduced in said microorganism by any means and methods known in
the art, preferably in a manner that after introduction the
promoter can increase the expression of said polynucleotide or that
said polynucleotide can be stably maintained and expressed by the
microorganism, respectively. Non-limiting examples of means and
methods for the introduction of a promoter sequence into a
microorganism may comprise, inter alia, recombinant homology as
known in the art (Ohm, loc cit). Non-limiting examples of such
methods for the introduction of a polynucleotide into a
microorganism may comprise transformation, transduction and
transfection as commonly known in the art and as also exemplified
herein (Sambrook and Russell (2001), Molecular Cloning: A
Laboratory Manual, CSH Press, Cold Spring Harbor, N.Y., USA;
Current Protocols in Molecular Biology, Update May 9, 2012, Print
ISSN: 1934-3639, Online ISSN: 1934-3647; Methods in Yeast Genetics,
A Laboratory Course Manual, Cold Spring Harbor Laboratory Press,
1990; van Peer, Applied Environ Microbiol (2009), 75: 1243-1247;
Schmid, "Genetics of Scleroglucan Production by Sclerotium
rolfsii", dissertation Technische Universitat Berlin, D83
(2008)).
[0019] In context with the present invention, the strong promoter
introduced into a microorganism upstream of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
preferably increases the expression level of said polynucleotide at
least 1.5-fold, more preferably at least 1.8-fold, more preferably
at least 2.0-fold, and most preferably at least 2.2-fold. In this
context, the expression level of a polynucleotide can be easily
assessed by the skilled person by methods known in the art, e.g.,
by quantitative RT-PCR, Northern Blot (for assessing the amount of
expressed mRNA levels), Dot Blot, Microarray or the like.
[0020] Generally, the term "overexpression" as used herein
comprises both, overexpression of polynucleotides (e.g., on the
transcriptional level) and overexpression of polypeptides (e.g., on
the translation level). Accordingly, the present invention relates
to a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain. In context
with the present invention, a genetically modified microorganism is
to be considered as "overexpressing" a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity if it
expresses at least 1.5-fold, more preferably at least 1.8-fold,
more preferably at least 2.0-fold, and most preferably at least
2.2-fold of said polynucleotide compared to a non-modified control
microorganism of the same strain. In this context, the expression
level of a polynucleotide can be easily assessed by the skilled
person by methods known in the art, e.g., by quantitative RT-PCR
(qRT-PCR), Northern Blot (for assessing the amount of expressed
mRNA levels), Dot Blot, Microarray or the like (see, e.g.,
Sambrook, loc cit; Current Protocols in Molecular Biology, Update
May 9, 2012, Print ISSN: 1934-3639, Online ISSN: 1934-3647).
Preferably, the amount of expressed polynucleotide is measured by
qRT-PCR. Furthermore, in context with the present invention, a
genetically modified microorganism is to be considered as
"overexpressing" a polypeptide having 1,3-.beta.-D-glucan
synthase-activity if it expresses at least 1.5-fold, more
preferably at least 1.8-fold, more preferably at least 2.0-fold,
and most preferably at least 2.2-fold of said polypeptide compared
to a non-modified control microorganism of the same strain. In this
context, the expression level of a polypeptide can be easily
assessed by the skilled person by methods known in the art, e.g.,
by Western Blot, ELISA, EIA, RIA, or the like (see, e.g., Sambrook,
loc cit; Current Protocols in Molecular Biology, Update May 9,
2012, Print ISSN: 1934-3639, Online ISSN: 1934-3647). Preferably,
the amount of expressed polypeptide is measured by Western
Blot.
[0021] Generally, in context with the present invention, the
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity may be introduced into the microorganism in any
suitable form, e.g., comprised in a vector, a plasmid or as naked
nucleic acid. The polynucleotide introduced into the microorganism
may then be exogenous (e.g., on a vector or a plasmid) within the
microorganism (i.e. outside of the microbial chromosome(s)), or it
may be incorporated into the microbial chromosome(s) by, e.g.,
random (ectopic) or homologous recombination or any other suitable
method as known in the art. In context with the present invention,
the polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity which has been introduced
into the microorganism (i.e. the additional copy to the natural
endogenous polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity of a corresponding unmodified
strain) does not necessarily have to have the same nucleotide
sequence as the natural endogenous polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity of a
corresponding unmodified strain, as long as it has
1,3-.beta.-D-glucan synthase-activity as described herein. Methods
for culturing microorganisms such as fermentation processes are
known in the art and also described and exemplified herein (Kumari,
Bioresource Technol (2008), 99: 1036-1043; Reyes, J Natural Studies
(2009), 7(2), January-June). In context with the present invention,
such methods allow the respective microorganism to grow and to
produce the desired .beta.-glucan as described and exemplified
herein. Suitable media may comprise, e.g., coconut water as
described in Reyes, loc cit. Furthermore, as known in the art,
there are several media particularly suitable for particular
microorganisms. For example, also in context with the present
invention, suitable media for culturing S. commune comprise CYM
medium (25 g agar (Difco), 20 g glucose (Sigma), 2 g trypticase
peptone (Roth), 2 g yeast extract (Difco), 0.5 g MgSO.sub.4.times.7
H.sub.2O (Roth), 0.5 g KH.sub.2PO.sub.4 and 1 g K.sub.2HPO.sub.4
(both from Riedel-de Haen) per liter H.sub.2O) (particularly useful
for cultivation on solid support) or a medium comprising 30 g
glucose (Sigma), 3 g yeast extract (Difco), 1 g KH.sub.2PO.sub.4
(Riedel-de Haen), 0.5 g MgSO.sub.4.times.7 H.sub.2O (Roth) per
liter H.sub.2O (particularly useful for liquid cultures) as also
described and exemplified herein. Further suitable media for
culturing S. rolfsii are known in the art (Survase, Bioresource
Technol (2006), 97: 989-993). The .beta.-glucan produced in
accordance to the present invention can be recovered by various
methods known in the art and described herein (see also
"Recommended Practices for Evaluation of Polymers Used in Enhanced
Oil Recovery Operations, API Recommended Practice 63 (RP 63),
1.sup.st Ed, American Petoleum Institute, Washington D.C., Jun. 1,
1990; Kumari, Bioresource Technol (2008), 99: 1036-1043).
[0022] In context with the present invention, the term "average
branching degree about 0.3" may mean that in average about 3 of
10.beta.-D-(1-3)-glucopyranosyl units are (1-6) linked to a single
.beta.-D-glucopyranosyl unit. In this context, the term "about" may
mean that the average branching degree may be within the range from
0.1 to 0.5, preferably from 0.2 to 0.4, more preferably from 0.25
to 0.35, more preferably from 0.25 to 0.33, more preferably from
0.27 to 0.33, and most preferably from 0.3 to 0.33. It may also be
0.3 or 0.33. Schizophyllan, scleroglucan, pendulan, cinerian,
laminarin, lentinan and pleuran all have an average branching
degree between 0.25 and 0.33; for example, scleroglucan and
schizophyllan have an average branching degree of 0.3 to 0.33
(Survase, loc cit; Novak, loc cit). The average branching degree of
a .beta.-glucan can be determined by methods known in the art,
e.g., by periodic oxidation analysis, methylated sugar analysis and
NMR (Brigand, Industrial Gums, Academic Press, New York/USA (1993),
461-472).
[0023] In one embodiment of the present invention, the polymer to
be produced is selected from the group consisting of schizophyllan,
scleroglucan, pendulan, cinerian, laminarin, lentinan and pleuran.
For example, the polymer may be schizophyllan or scleroglucan,
particularly schizophyllan.
[0024] The microorganism of the present invention and as referred
to and as employed in context with the present invention
(hereinafter also referred to as "microorganism in context of the
present invention") may generally be a microorganism which is per
se (i.e. naturally, in a non-modified state in context with the
present invention) capable of synthesizing .beta.-glucan polymers,
particularly those polymers consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3. That is, such microorganisms
preferably possess per se (i.e. naturally, in a non-modified state
in context with the present invention) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity.
Non-limiting examples of microorganisms in context of the present
invention are Schizophyllum commune, Sclerotium rolfsii, Sclerotium
glucanicum, Sclerotium delphinii, Porodisculus pendulus, Botrytis
cinerea, Laminaria sp., Lentinula edoles, and Monilinia fructigena.
For example, the microorganism in context with the present
invention may be S. commune or S. rolfsii, particularly S.
commune.
[0025] The polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity as referred to and to be
employed in context with the present invention (hereinafter also
referred to as the "polynucleotide in context of the present
invention") may be a 1,3-.beta.-D-glucan synthase gene. For
example, the polynucleotide in context of the present invention may
comprise or may consist of a nucleic acid sequence which is at
least 70%, preferably at least 75%, more preferably at least 80%,
more preferably at least 85%, more preferably at least 90%, more
preferably at least 95%, more preferably at least 96%, more
preferably at least 97%, more preferably at least 98%, more
preferably at least 99%, more preferably at least 99.5%, and most
preferably 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or
15, provided that the polypeptide encoded by said polynucleotide
has 1,3-.beta.-D-glucan synthase-activity as further described and
exemplified herein below. SEQ ID NO: 1 represents the nucleotide
sequence of the gene of glucan synthase I of S. commune strain
Lu15531 (obtained from Jena University (Germany) strain collection,
Germany, Prof. E. Kothe; Jena University internal strain name:
W22). SEQ ID NO: 3 represents the nucleotide sequence of the gene
of glucan synthase II of S. commune strain Lu15531. SEQ ID NO: 5
represents the cDNA sequence of glucan synthase I of S. commune
strain Lu15531. SEQ ID NO: 7 represents the cDNA sequence of glucan
synthase II of S. commune strain Lu15531. SEQ ID NO: 9 represents
the nucleotide sequence of the gene of glucan synthase I of S.
commune strain Lu15634 (strain collection, BASF SE; monocaryotic
strain originating from dicaryotic S. commune strain from strain
collection at the Technical University of Braunschweig (Germany),
Prof. Rau; generated by spore isolation). SEQ ID NO: 11 represents
the nucleotide sequence of the gene of glucan synthase II of S.
commune strain Lu15634. SEQ ID NO: 13 represents the cDNA sequence
of glucan synthase I of S. commune strain Lu15634. SEQ ID NO: 15
represents the cDNA sequence of glucan synthase II of S. commune
strain Lu15634.
[0026] The polypeptide as referred to and to be used in context
with the present invention and the polypeptide encoded by the
polynucleotide in context of the present invention (said
polypeptides hereinafter also referred to as the "polypeptide in
context of the present invention") has 1,3-.beta.-D-glucan
synthase-activity. In one embodiment, it is a 1,3-.beta.-D-glucan
synthase. For example, the polypeptide in context of the present
invention may comprise or consist of an amino acid sequence which
at least 70%, preferably at least 75%, more preferably at least
80%, more preferably at least 85%, more preferably at least 90%,
more preferably at least 95%, more preferably at least 96%, more
preferably at least 97%, more preferably at least 98%, more
preferably at least 99%, more preferably at least 99.5%, and most
preferably 100% identical to SEQ ID NO: 6, 8, 14 or 16, provided
that the polypeptide has 1,3-.beta.-D-glucan synthase-activity. SEQ
ID NO: 6 represents the amino acid sequence of glucan synthase I of
S. commune strain Lu15531. SEQ ID NO: 8 represents the amino acid
sequence of glucan synthase II of S. commune strain Lu15531. SEQ ID
NO: 14 represents the amino acid sequence of glucan synthase I of
S. commune strain Lu15634. SEQ ID NO: 16 represents the amino acid
sequence of glucan synthase II of S. commune strain Lu15634.
[0027] In context with the present invention, the term
"1,3-.beta.-D-glucan synthase-activity" means that the respective
polypeptide is capable of catalyzing the elongation of the
1,3-.beta.-D-glucan chain (chain can be linear or branched) using
UDP-glucose as substrate (see Inoue, Eur J Biochem (1995), 231:
845-854). For example, in context with the present invention, a
polynucleotide may be considered to encode a polypeptide having
1,3-.beta.-D-glucan synthase-activity if an S. commune cell which
is transformed with said polynucleotide and which expresses said
polynucleotide constitutively is able to produce at least 50%, more
preferably at least 75%, more preferably at least 100%, more
preferably at least 120%, more preferably at least 150%, more
preferably at least 200%, and most preferably at least 220% more
schizophyllan compared to an S. commune cell not being transformed
with said polynucleotide, wherein the following conditions may be
applied. The respective S. commune cultures with transformed and
non-transformed cells, respectively, may be cultivated as follows.
For the liquid cultures, the following medium may be used
(hereinafter referred to as "Standard Medium"): 30 g glucose
(Sigma), 3 g yeast extract (Difco), 1 g KH.sub.2PO.sub.4 (Riedel-de
Haen), 0.5 g MgSO.sub.4.times.7 H.sub.2O (Roth) per liter H.sub.2O.
For both, pre-cultures and for main culture, 250 ml shaking flasks
filled with 30 ml Standard Medium may be used. The cultivation may
be carried out at 27.degree. C. and 225 rpm. Before each
inoculation, the biomass may be homogenized for 1 minute at 13500
rpm using T 25 digital ULTRA-TURRAX.RTM. (IKA). The first
pre-culture may be inoculated with 50 mg of wet biomass. The
cultures may then be incubated for 72 hours. After 72 hours, the
second pre-culture may be started. The concentration of the
homogenized wet biomass from the first pre-culture used for
inoculation may be 250 mg. Cultivation time may be 45 hours. After
45 hours, the main culture may be inoculated with 500 mg of
homogenized wet biomass from the second pre-culture and cultivated
for another 45 hours. Subsequently, the cultures may be treated as
follows. 10 ml of the culture, 20 ml H.sub.2O and 90 .mu.l Acticide
BW20 may be mixed. The sample may then be digested for 24 h at
40.degree. C. with .beta.-glucanase (0.3 ml) (Erbsloh). After the
incubation, the sample may be centrifuged (e.g., 30 minutes at 3400
g) and the supernatant may be analyzed for glucose content using
HPLC cation exchanger (Aminex HPX-87-H, BIO-RAD) with 0.5 M
H.sub.2SO.sub.4 (Roth) as eluent and 0.5 ml/min flow rate at
30.degree. C. The typical schizophyllan structure as described
herein may be confirmed by further analytical approaches as
described in the Example herein below (e.g., by NMR and XRD). The
same evaluation may be performed mutatis mutandis for assessing
whether a given polypeptide has 1,3-.beta.-D-glucan
synthase-activity in context of the present invention. In this
case, a corresponding polynucleotide encoding said polypeptide to
be assessed is evaluated mutatis mutandis as described above. If
the expression of such a polynucleotide encoding said polypeptide
to be assessed is considered to encode a polypeptide having
1,3-.beta.-D-glucan synthase-activity as described above, the
polypeptide itself is considered to have 1,3-.beta.-D-glucan
synthase-activity.
[0028] The level of identity between two or more sequences (e.g.,
nucleic acid sequences or amino acid sequences) can be easily
determined by methods known in the art, e.g., by BLAST analysis.
Generally, in context with the present invention, if two sequences
(e.g., polynucleotide sequences or amino acid sequences) to be
compared by, e.g., sequence comparisons differ in identity, then
the term "identity" may refer to the shorter sequence and that part
of the longer sequence that matches said shorter sequence.
Therefore, when the sequences which are compared do not have the
same length, the degree of identity may preferably either refer to
the percentage of nucleotide residues in the shorter sequence which
are identical to nucleotide residues in the longer sequence or to
the percentage of nucleotides in the longer sequence which are
identical to nucleotide sequence in the shorter sequence. In this
context, the skilled person is readily in the position to determine
that part of a longer sequence that matches the shorter sequence.
Furthermore, as used herein, identity levels of nucleic acid
sequences or amino acid sequences may refer to the entire length of
the respective sequence and is preferably assessed pair-wise,
wherein each gap is to be counted as one mismatch. These
definitions for sequence comparisons (e.g., establishment of
"identity" values) are to be applied for all sequences described
and disclosed herein.
[0029] Moreover, the term "identity" as used herein means that
there is a functional and/or structural equivalence between the
corresponding sequences. Nucleic acid/amino acid sequences having
the given identity levels to the herein-described particular
nucleic acid/amino acid sequences may represent
derivatives/variants of these sequences which, preferably, have the
same biological function. They may be either naturally occurring
variations, for instance sequences from other varieties, species,
etc., or mutations, and said mutations may have formed naturally or
may have been produced by deliberate mutagenesis. Furthermore, the
variations may be synthetically produced sequences. The variants
may be naturally occurring variants or synthetically produced
variants or variants produced by recombinant DNA techniques.
Deviations from the above-described nucleic acid sequences may have
been produced, e.g., by deletion, substitution, addition, insertion
and/or recombination. The term "addition" refers to adding at least
one nucleic acid residue/amino acid to the end of the given
sequence, whereas "insertion" refers to inserting at least one
nucleic acid residue/amino acid within a given sequence. The term
"deletion" refers to deleting or removal of at least one nucleic
acid residue or amino acid residue in a given sequence. The term
"substitution" refers to the replacement of at least one nucleic
acid residue/amino acid residue in a given sequence. Again, these
definitions as used here apply, mutatis mutandis, for all sequences
provided and described herein.
[0030] Generally, as used herein, the terms "polynucleotide" and
"nucleic acid" or "nucleic acid molecule" are to be construed
synonymously. Generally, nucleic acid molecules may comprise inter
alia DNA molecules, RNA molecules, oligonucleotide thiophosphates,
substituted ribo-oligonucleotides or PNA molecules. Furthermore,
the term "nucleic acid molecule" may refer to DNA or RNA or hybrids
thereof or any modification thereof that is known in the art (see,
e.g., U.S. Pat. No. 5,525,711, US 471 1955, U.S. Pat. No. 5,792,608
or EP 302175 for examples of modifications). The polynucleotide
sequence may be single- or double-stranded, linear or circular,
natural or synthetic, and without any size limitation. For
instance, the polynucleotide sequence may be genomic DNA, cDNA,
mitochondrial DNA, mRNA, antisense RNA, ribozymal RNA or a DNA
encoding such RNAs or chimeroplasts (Gamper, Nucleic Acids
Research, 2000, 28, 4332-4339). Said polynucleotide sequence may be
in the form of a vector, plasmid or of viral DNA or RNA. Also
described herein are nucleic acid molecules which are complementary
to the nucleic acid molecules described above and nucleic acid
molecules which are able to hybridize to nucleic acid molecules
described herein. A nucleic acid molecule described herein may also
be a fragment of the nucleic acid molecules in context of the
present invention. Particularly, such a fragment is a functional
fragment. Examples for such functional fragments are nucleic acid
molecules which can serve as primers.
[0031] The term "hybridization" or "hybridizes" as used herein in
context of nucleic acid molecules/DNA sequences may relate to
hybridizations under stringent or non-stringent conditions. If not
further specified, the conditions are preferably non-stringent.
Said hybridization conditions may be established according to
conventional protocols described, for example, in Sambrook, Russell
"Molecular Cloning, A Laboratory Manual", Cold Spring Harbor
Laboratory, N. Y. (2001); Current Protocols in Molecular Biology,
Update May 9, 2012, Print ISSN: 1934-3639, Online ISSN: 1934-3647;
Ausubel, "Current Protocols in Molecular Biology", Green Publishing
Associates and Wiley Interscience, N. Y. (1989), or Higgins and
Hames (Eds.) "Nucleic acid hybridization, a practical approach" IRL
Press Oxford, Washington D.C., (1985). The setting of conditions is
well within the skill of the artisan and can be determined
according to protocols described in the art. Thus, the detection of
only specifically hybridizing sequences will usually require
stringent hybridization and washing conditions such as
0.1.times.SSC, 0.1% SDS at 65.degree. C. Non-stringent
hybridization conditions for the detection of homologous or not
exactly complementary sequences may be set at 6.times.SSC, 1% SDS
at 65.degree. C. As is well known, the length of the probe and the
composition of the nucleic acid to be determined constitute further
parameters of the hybridization conditions. Variations in the above
conditions may be accomplished through the inclusion and/or
substitution of alternate blocking reagents used to suppress
background in hybridization experiments. Typical blocking reagents
include Denhardt's reagent, BLOTTO, heparin, denatured salmon sperm
DNA, and commercially available proprietary formulations. The
inclusion of specific blocking reagents may require modification of
the hybridization conditions described above, due to problems with
compatibility. In accordance to the invention described herein, low
stringent hybridization conditions for the detection of homologous
or not exactly complementary sequences may, for example, be set at
6.times.SSC, 1% SDS at 65.degree. C. As is well known, the length
of the probe and the composition of the nucleic acid to be
determined constitute further parameters of the hybridization
conditions.
[0032] Hybridizing nucleic acid molecules also comprise fragments
of the above described molecules. Such fragments may represent
nucleic acid molecules which code for a functional
1,3-.beta.-D-glucan synthase as described herein or a functional
fragment thereof which can serve as a primer. Furthermore, nucleic
acid molecules which hybridize with any of the aforementioned
nucleic acid molecules also include complementary fragments,
derivatives and variants of these molecules. Additionally, a
hybridization complex refers to a complex between two nucleic acid
sequences by virtue of the formation of hydrogen bonds between
complementary G and C bases and between complementary A and T
bases; these hydrogen bonds may be further stabilized by base
stacking interactions. The two complementary nucleic acid sequences
hydrogen bond in an antiparallel configuration. A hybridization
complex may be formed in solution (e.g., Cot or Rot analysis) or
between one nucleic acid sequence present in solution and another
nucleic acid sequence immobilized on a solid support (e.g.,
membranes, filters, chips, pins or glass slides to which, e.g.,
cells have been fixed). The terms complementary or complementarity
refer to the natural binding of polynucleotides under permissive
salt and temperature conditions by base-pairing. For example, the
sequence "A-G-T" binds to the complementary sequence "T-C-A".
Complementarity between two single-stranded molecules may be
"partial", in which only some of the nucleic acids bind, or it may
be complete when total complementarity exists between
single-stranded molecules. The degree of complementarity between
nucleic acid strands has significant effects on the efficiency and
strength of hybridization between nucleic acid strands. This is of
particular importance in amplification reactions, which depend upon
binding between nucleic acids strands. The term "hybridizing
sequences" preferably refers to sequences which display a sequence
identity of at least 45%, more preferably at least 50%, more
preferably at least 55%, more preferably at least 60%, more
preferably at least 65%, more preferably at least 70%, more
preferably at least 75%, more preferably at least 80%. more
preferably at least 85%, more preferably at least 90%, more
preferably at least 95%, more preferably at least 96%, more
preferably at least 97%, more preferably at least 98% more
preferably at least 99%, more preferably at least 99.5%, and most
preferably 100% identity with a nucleic acid sequence as described
herein encoding a 1,3-.beta.-D-glucan synthase.
[0033] Also described herein are vectors containing a
polynucleotide in context of the present invention. The present
invention relates also to a vector comprising the polynucleotide in
context of the present invention. The term "vector" as used herein
particularly refers to plasmids, cosmids, viruses, bacteriophages
and other vectors commonly used in genetic engineering. In a
preferred embodiment, the vectors are suitable for the
transformation, transduction and/or transfection of microorganisms
as described herein, e.g., fungal cells, prokaryotic ells (e.g.,
bacteria), yeast, and the like. Specific examples of microorganisms
in context with the present invention are Schizophyllum commune,
Sclerotium rolfsii, Sclerotium glucanicum, Sclerotium delphinii,
Porodisculus pendulus, Botrytis cinerea, Laminaria sp., Lentinula
edoles, and Monilinia fructigena. In a particularly preferred
embodiment, said vectors are suitable for stable transformation of
the microorganism, for example to express the polypeptide having
1,3-.beta.-D-glucan synthase activity as described herein.
Accordingly, in one aspect of the invention, the vector as provided
is an expression vector. Generally, expression vectors have been
widely described in the literature. As a rule, they may not only
contain a selection marker gene and a replication-origin ensuring
replication in the host selected, but also a promoter, and in most
cases a termination signal for transcription. Between the promoter
and the termination signal there is preferably at least one
restriction site or a polylinker which enables the insertion of a
nucleic acid sequence/molecule desired to be expressed.
[0034] It is to be understood that when the vector provided herein
is generated by taking advantage of an expression vector known in
the prior art that already comprises a promoter suitable to be
employed in context of this invention, for example expression of a
polypeptide having 1,3-.beta.-D-glucan synthase activity as
described herein. The nucleic acid construct is preferably inserted
into that vector in a manner the resulting vector comprises only
one promoter suitable to be employed in context of this invention.
The skilled person knows how such insertion can be put into
practice. For example, the promoter can be excised either from the
nucleic acid construct or from the expression vector prior to
ligation. A non-limiting example of the vector of the present
invention is pBluescript II comprising the polynucleotide in
context of the present invention. Further examples of vectors
suitable to comprise the polynucleotide in context of the present
invention to form the described herein are known in the art and
comprise, for example pDrive, pTOPO, pUC19 and pUC21.
[0035] Generally, the present invention relates to all the
embodiments described herein as well as to all permutations and
combinations thereof. The following particular aspects of the
present invention must not be construed as limiting the scope of
the present invention on such aspects.
[0036] In one aspect, the present invention relates to a
genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain.
[0037] In one aspect, the present invention relates to a
genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain.
[0038] In another aspect, the present invention relates to a
genetically modified microorganism of the species Schizoyphyllum
commune, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain.
[0039] In another aspect, the present invention relates to a
genetically modified microorganism of the species Schizoyphyllum
commune, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain.
[0040] In one aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain.
[0041] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain.
[0042] In another aspect, the present invention relates to a
genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain.
[0043] In another aspect, the present invention relates to a
genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain.
[0044] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15.
[0045] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15.
[0046] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0047] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0048] In another aspect, the present invention relates to a
genetically modified microorganism of the species Schizoyphyllum
commune, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0049] In another aspect, the present invention relates to a
genetically modified microorganism of the species Schizoyphyllum
commune, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0050] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0051] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0052] In another aspect, the present invention relates to a
genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0053] In another aspect, the present invention relates to a
genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.
[0054] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or
16.
[0055] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or
16.
[0056] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0057] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0058] In another aspect, the present invention relates to a
genetically modified microorganism of the species Schizoyphyllum
commune, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0059] In another aspect, the present invention relates to a
genetically modified microorganism of the species Schizoyphyllum
commune, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0060] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0061] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0062] In another aspect, the present invention relates to a
genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0063] In another aspect, the present invention relates to a
genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16.
[0064] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing
schizophyllan.
[0065] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing
scleroglucan.
[0066] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, wherein said polynucleotide
is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%,
99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or
15.
[0067] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, wherein said polypeptide is
at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%,
or 100% identical to SEQ ID NO: 6, 8, 14 or 16.
[0068] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing schizophyllan,
wherein said polynucleotide is at least 70%, 75%, 80%, 85%, 90%,
95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1,
3, 5, 7, 9, 11, 13 or 15.
[0069] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing schizophyllan,
wherein said polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%,
96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14
or 16.
[0070] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing scleroglucan,
wherein said polynucleotide is at least 70%, 75%, 80%, 85%, 90%,
95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1,
3, 5, 7, 9, 11, 13 or 15.
[0071] In another aspect, the present invention relates to the use
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity for producing scleroglucan,
wherein said polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%,
96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14
or 16.
[0072] In another aspect, the present invention relates to the use
of a polypeptide having 1,3-.beta.-D-glucan synthase-activity for
producing schizophyllan.
[0073] In another aspect, the present invention relates to the use
of a polypeptide having 1,3-.beta.-D-glucan synthase-activity for
producing scleroglucan.
[0074] In another aspect, the present invention relates to the use
of polypeptide having 1,3-.beta.-D-glucan synthase-activity for
producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, wherein said polypeptide is
at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%,
or 100% identical to SEQ ID NO: 6, 8, 14 or 16.
[0075] In another aspect, the present invention relates to the use
of a polypeptide having 1,3-.beta.-D-glucan synthase-activity for
producing schizophyllan, wherein said polypeptide is at least 70%,
75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%
identical to SEQ ID NO: 6, 8, 14 or 16.
[0076] In another aspect, the present invention relates to the use
of polypeptide having 1,3-.beta.-D-glucan synthase-activity for
producing scleroglucan, wherein said polypeptide is at least 70%,
75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%
identical to SEQ ID NO: 6, 8, 14 or 16.
[0077] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, wherein said genetically modified microorganism
overexpresses (i) a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity, and/or (ii) a polypeptide
having 1,3-.beta.-D-glucan synthase-activity, compared to a
corresponding non-modified control microorganism of the same
strain, for producing a polymer consisting of a linear main chain
of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0078] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, wherein said genetically modified microorganism
contains at least one copy more of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity compared
to a corresponding non-modified control microorganism of the same
strain, for producing a polymer consisting of a linear main chain
of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0079] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, wherein said genetically modified microorganism
overexpresses (i) a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity, and/or (ii) a polypeptide
having 1,3-.beta.-D-glucan synthase-activity, compared to a
corresponding non-modified control microorganism of the same
strain, for producing schizophyllan.
[0080] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, wherein said genetically modified microorganism
contains at least one copy more of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity compared
to a corresponding non-modified control microorganism of the same
strain, for producing schizophyllan.
[0081] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, wherein said genetically modified microorganism
overexpresses (i) a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity, and/or (ii) a polypeptide
having 1,3-.beta.-D-glucan synthase-activity, compared to a
corresponding non-modified control microorganism of the same
strain, for producing scleroglucan.
[0082] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, wherein said genetically modified microorganism
contains at least one copy more of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity compared
to a corresponding non-modified control microorganism of the same
strain, for producing scleroglucan. In another aspect, the present
invention relates to the use of a genetically modified
microorganism of the species Schizoyphyllum commune, characterized
in that said genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, for
producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0083] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0084] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for producing schizophyllan.
[0085] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, for producing schizophyllan.
[0086] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for producing scleroglucan.
[0087] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, for producing scleroglucan.
[0088] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for producing a polymer consisting of a linear
main chain of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0089] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, for producing a polymer consisting of a linear
main chain of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0090] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for producing schizophyllan.
[0091] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, for producing schizophyllan.
[0092] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for producing scleroglucan.
[0093] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, for producing scleroglucan.
[0094] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for producing a polymer consisting of a linear
main chain of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0095] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, for producing a polymer consisting of a linear
main chain of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0096] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for schizophyllan.
[0097] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, for schizophyllan.
[0098] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, for scleroglucan.
[0099] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, for scleroglucan.
[0100] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15, for producing a polymer consisting of a linear main chain
of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0101] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15, for producing a polymer consisting of a linear main chain
of .beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0102] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15, for producing schizophyllan.
[0103] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15, for producing schizophyllan.
[0104] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15, for producing scleroglucan.
[0105] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15, for producing scleroglucan.
[0106] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0107] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0108] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0109] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0110] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0111] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0112] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0113] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, wherein said polynucleotide is at
least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or
100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for
producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0114] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0115] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, wherein said polynucleotide is at
least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or
100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for
producing schizophyllan.
[0116] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0117] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, wherein said polynucleotide is at
least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or
100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for
producing scleroglucan.
[0118] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0119] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0120] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0121] In another aspect, the present invention relates to a
genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0122] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0123] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0124] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0125] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing a polymer
consisting of a linear main chain of .beta.-D-(1-3)-glucopyranosyl
units having a single .beta.-D-glucopyranosyl unit (1-6) linked to
a .beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0126] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0127] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
schizophyllan.
[0128] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0129] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polynucleotide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15, for producing
scleroglucan.
[0130] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16,
for producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0131] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16,
for producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0132] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16,
for producing schizophyllan.
[0133] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16,
for producing schizophyllan.
[0134] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism overexpresses (i) a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity, and/or (ii) a polypeptide having
1,3-.beta.-D-glucan synthase-activity, compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16,
for producing scleroglucan.
[0135] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, characterized in that said
genetically modified microorganism contains at least one copy more
of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity compared to a corresponding
non-modified control microorganism of the same strain, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16,
for producing scleroglucan.
[0136] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0137] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0138] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0139] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0140] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0141] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
schizophyllan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0142] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0143] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, wherein said polypeptide is at
least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or
100% identical to SEQ ID NO: 6, 8, 14 or 16, for producing a
polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0144] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0145] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, wherein said polypeptide is at
least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or
100% identical to SEQ ID NO: 6, 8, 14 or 16, for producing
schizophyllan.
[0146] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism overexpresses (i) a polynucleotide encoding
a polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0147] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species
Schizoyphyllum commune, characterized in that said genetically
modified microorganism contains at least one copy more of a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity compared to a corresponding non-modified control
microorganism of the same strain, wherein said polypeptide is at
least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or
100% identical to SEQ ID NO: 6, 8, 14 or 16, for producing
scleroglucan.
[0148] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0149] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0150] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0151] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0152] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0153] In another aspect, the present invention relates to the use
of a genetically modified microorganism capable of producing
scleroglucan, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0154] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0155] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing a polymer consisting of a
linear main chain of .beta.-D-(1-3)-glucopyranosyl units having a
single .beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3.
[0156] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0157] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing schizophyllan.
[0158] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism overexpresses (i) a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity, and/or
(ii) a polypeptide having 1,3-.beta.-D-glucan synthase-activity,
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0159] In another aspect, the present invention relates to the use
of a genetically modified microorganism of the species Sclerotium
rolfsii, characterized in that said genetically modified
microorganism contains at least one copy more of a polynucleotide
encoding a polypeptide having 1,3-.beta.-D-glucan synthase-activity
compared to a corresponding non-modified control microorganism of
the same strain, wherein said polypeptide is at least 70%, 75%,
80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to
SEQ ID NO: 6, 8, 14 or 16, for producing scleroglucan.
[0160] In another aspect, the present invention relates to a method
of producing schizophyllan, said method comprising the steps of:
[0161] (a) introducing a strong (e.g., constitutive or inducible)
promoter upstream of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity thereby increasing the
expression of said polynucleotide, or a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism being able to synthesize schizophyllan; [0162] (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce schizophyllan; and [0163] (c)
optionally recovering schizophyllan from the medium.
[0164] In another aspect, the present invention relates to a method
of producing scleroglucan, said method comprising the steps of:
[0165] (a) introducing a strong (e.g., constitutive or inducible)
promoter upstream of a polynucleotide encoding a polypeptide having
1,3-.beta.-D-glucan synthase-activity thereby increasing the
expression of said polynucleotide, or a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism being able to synthesize scleroglucan; [0166] (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce scleroglucan; and [0167] (c)
optionally recovering scleroglucan from the medium.
[0168] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0169] (a) introducing a strong (e.g., constitutive or
inducible) promoter upstream of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity thereby
increasing the expression of said polynucleotide, or a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity into a microorganism of the species Schizophyllum
commune being able to synthesize said polymer; [0170] (b) culturing
said microorganism of (a) in a medium, thereby allowing said
microorganism to produce said polymer; and [0171] (c) optionally
recovering said polymer from the medium.
[0172] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0173] (a) introducing a strong (e.g., constitutive or
inducible) promoter upstream of a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity thereby
increasing the expression of said polynucleotide, or a
polynucleotide encoding a polypeptide having 1,3-.beta.-D-glucan
synthase-activity into a microorganism of the species Sclerotium
rolfsii being able to synthesize said polymer; [0174] (b) culturing
said microorganism of (a) in a medium, thereby allowing said so
microorganism to produce said polymer; and [0175] (c) optionally
recovering said polymer from the medium.
[0176] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0177] (a) introducing a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism being able to synthesize said polymer, wherein said
polynucleotide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11,
13 or 15; [0178] (b) culturing said microorganism of (a) in a
medium, thereby allowing said microorganism to produce said
polymer; and [0179] (c) optionally recovering said polymer from the
medium.
[0180] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0181] (a) introducing a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism being able to synthesize said polymer, wherein said
polypeptide is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%,
98%, 99%, 99.5%, or 100% identical to SEQ ID NO: 6, 8, 14 or 16;
[0182] (b) culturing said microorganism of (a) in a medium, thereby
allowing said microorganism to produce said polymer; and [0183] (c)
optionally recovering said polymer from the medium.
[0184] In another aspect, the present invention relates to a method
of producing schizophyllan, said method comprising the steps of:
[0185] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
being able to synthesize schizophyllan, wherein said polynucleotide
is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%,
99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15;
[0186] (b) culturing said microorganism of (a) in a medium, thereby
allowing said microorganism to produce schizophyllan; and [0187]
(c) optionally recovering schizophyllan from the medium.
[0188] In another aspect, the present invention relates to a method
of producing scleroglucan, said method comprising the steps of:
[0189] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
being able to synthesize scleroglucan, wherein said polynucleotide
is at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%,
99.5%, or 100% identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15;
[0190] (b) culturing said microorganism of (a) in a medium, thereby
allowing said microorganism to produce scleroglucan; and [0191] (c)
optionally recovering scleroglucan from the medium.
[0192] In another aspect, the present invention relates to a method
of producing schizophyllan, said method comprising the steps of:
[0193] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
being able to synthesize schizophyllan, wherein said polypeptide is
at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%,
or 100% identical to SEQ ID NO: 6, 8, 14 or 16; [0194] (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce schizophyllan; and [0195] (c)
optionally recovering schizophyllan from the medium.
[0196] In another aspect, the present invention relates to a method
of producing scleroglucan, said method comprising the steps of:
[0197] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
being able to synthesize scleroglucan, wherein said polypeptide is
at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%,
or 100% identical to SEQ ID NO: 6, 8, 14 or 16; [0198] (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce scleroglucan; and [0199] (c)
optionally recovering scleroglucan from the medium.
[0200] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0201] (a) introducing a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism of the species Schizophyllum commune being able to
synthesize said polymer, wherein said polynucleotide is at least
70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%
identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15; [0202] (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce said polymer; and [0203] (c)
optionally recovering said polymer from the medium.
[0204] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0205] (a) introducing a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism of the species Sclerotium rolfsii being able to
synthesize said polymer, wherein said polynucleotide is at least
70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%
identical to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15; [0206] (b)
culturing said microorganism of (a) in a medium, thereby allowing
said microorganism to produce said polymer; and [0207] (c)
optionally recovering said polymer from the medium.
[0208] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0209] (a) introducing a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism of the species Schizophyllum commune being able to
synthesize said polymer, wherein said polypeptide is at least 70%,
75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%
identical to SEQ ID NO: 6, 8, 14 or 16; [0210] (b) culturing said
microorganism of (a) in a medium, thereby allowing said
microorganism to produce said polymer; and [0211] (c) optionally
recovering said polymer from the medium.
[0212] In another aspect, the present invention relates to a method
of producing a polymer consisting of a linear main chain of
.beta.-D-(1-3)-glucopyranosyl units having a single
.beta.-D-glucopyranosyl unit (1-6) linked to a
.beta.-D-glucopyranosyl unit of the linear main chain with an
average branching degree of about 0.3, said method comprising the
steps of: [0213] (a) introducing a polynucleotide encoding a
polypeptide having 1,3-.beta.-D-glucan synthase-activity into a
microorganism of the species Sclerotium rolfsii being able to
synthesize said polymer, wherein said polypeptide is at least 70%,
75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%
identical to SEQ ID NO: 6, 8, 14 or 16; [0214] (b) culturing said
microorganism of (a) in a medium, thereby allowing said
microorganism to produce said polymer; and [0215] (c) optionally
recovering said polymer from the medium.
[0216] In another aspect, the present invention relates to a method
of producing schizophyllan, said method comprising the steps of:
[0217] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
of the species Schizophyllum commune being able to synthesize said
polymer, wherein said polynucleotide is at least 70%, 75%, 80%,
85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ
ID NO: 1, 3, 5, 7, 9, 11, 13 or 15; [0218] (b) culturing said
microorganism of (a) in a medium, thereby allowing said
microorganism to produce said polymer; and [0219] (c) optionally
recovering said polymer from the medium.
[0220] In another aspect, the present invention relates to a method
of producing scleroglucan, said method comprising the steps of:
[0221] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
of the species Sclerotium rolfsii being able to synthesize said
polymer, wherein said polynucleotide is at least 70%, 75%, 80%,
85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ
ID NO: 1, 3, 5, 7, 9, 11, 13 or 15; [0222] (b) culturing said
microorganism of (a) in a medium, thereby allowing said
microorganism to produce said polymer; and [0223] (c) optionally
recovering said polymer from the medium.
[0224] In another aspect, the present invention relates to a method
of producing schizophyllan, said method comprising the steps of:
[0225] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
of the species Schizophyllum commune being able to synthesize said
polymer, wherein said polypeptide is at least 70%, 75%, 80%, 85%,
90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ ID
NO: 6, 8, 14 or 16; [0226] (b) culturing said microorganism of (a)
in a medium, thereby allowing said microorganism to produce said
polymer; and [0227] (c) optionally recovering said polymer from the
medium.
[0228] In another aspect, the present invention relates to a method
of producing scleroglucan, said method comprising the steps of:
[0229] (a) introducing a polynucleotide encoding a polypeptide
having 1,3-.beta.-D-glucan synthase-activity into a microorganism
of the species Sclerotium rolfsii being able to synthesize said
polymer, wherein said polypeptide is at least 70%, 75%, 80%, 85%,
90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% identical to SEQ ID
NO: 6, 8, 14 or 16; [0230] (b) culturing said microorganism of (a)
in a medium, thereby allowing said microorganism to produce said
polymer; and [0231] (c) optionally recovering said polymer from the
medium.
[0232] The Figures show:
[0233] FIG. 1 XRD Spectrum of Schizophyllan sample. The triple
helix could be seen as an intensive diffraction at 5.degree.
2.theta. and the amorphous region of the material gives broad
diffraction in the range of 20-25.degree. 2.theta.
[0234] FIG. 2 .sup.1H-NMR of schizophyllan (50 mg of gel) in
[D.sub.6]-DMSO measured at 50.degree. C. (16 scans, 600 MHz). The
substitution pattern of schizophyllan can be assigned from the
integrations of the CH.sub.2OH at 3.7 ppm and CH.sub.2O (ether) at
4.1 ppm signals, the ratio was determined to be 3.3:1 indicating
the correct repeating unit.
[0235] FIG. 3 13C-NMR of schizophyllan (50 mg of gel) in [D6]-DMSO
measured at 50.degree. C. (10.000 scans, 600 MHz). Assignment of
the signals, .delta. (ppm): 60 and 61 (C-6), 68 (C6-C .beta.(1-6)),
68 (C4-OH side glucose), 70 (C-2 backbone), 72 (C-2), 76 (C-5),
76.7 (C-3 side glucose), 86 (c-.sctn.backbone), 103 (C-1).
[0236] FIG. 4 Schematic picture of the repeating unit of
schizophyllan.
[0237] The following Examples illustrate the present invention.
Yet, the present invention must not be construed as being limited
by the following Examples.
EXAMPLES
Example 1
Cloning of the .beta.-1,3-Glucan Synthase Expression Plasmid
(pGS_1) and Transformation into S. commune
[0238] In the genome of Schizophyllum commune, two genes encoding
for .beta.-1,3-glucan synthase were identified by using BLAST
analysis (query genes: 1,3-.beta.-glucan synthase sequence from
Mycosphaerella graminicola, Saccharomyces cerevisiae, Cryptococcus
neoformans, Schizosaccharomyces pombe); cf. Ullman, Biochem J
(1997), 326: 929-942. In context of the present invention, it was
proven that the overexpression of either of these .beta.-1,3-glucan
synthases in S. commune results in increased yields of
schizophyllan production.
[0239] Two expression plasmids (pGS_1)] and (pGS_2) (having
pBluescript II as backbone) were generated carrying selection
marker cassette (amp.sup.R, ura1), strong constitutive promoter
(Tef1 promoter), the synthase gene sequence (genomic seqence) and
terminator sequence (Tef1 terminator).
[0240] All polynucleotide sequences described herein originate from
Schizopyllum commune. The polynucleotides represented by SEQ ID NO:
1 and 3 (genes .beta.-1,3-glucan synthases I and II of Lu15531,
respectively) were synthesized by Eurofins MWG GmbH/Germany
(eurofinsdna.com/de) according to the original sequence data
sourced from JGI data base (jgi.doe.gov/Scommune; gene position:
scaffold 2, 1194740-1200474 and gene position: scaffold 6,
1391067-1396555). The sequences were delivered on pMK plasmids
(pMK_GS_1) and (pMK_GS_2) (Eurofins plasmids containing kan.sup.R,
ColE1 origin and genomic sequence of respective .beta.-1,3-glucan
synthases). The polynucleotides were further used for the cloning
of the complete expression plasmid. Plasmid (pMK_GS_1) contained a
polynucleotide represented by SEQ ID NO: 1 flanked by 5' SpeI and
3' SalI restriction sites. Plasmid (pMK_GS_2) contained a
polynucleotide represented by SEQ ID NO: 3 flanked by 5' SpeI and
3' EcoRV restriction sites, respectively.
[0241] The individual elements (SEQ ID NO: 17, 18 and 33 (Tef1
promoter, Tef1 terminator and ura1) were isolated from the genomic
DNA of Schizophyllum commune using PCR technology prepared by
established microbiologic protocols (Sambrook, Current Protocols in
Molecular Biology, Update May 9, 2012, Print ISSN: 1934-3639,
Online ISSN: 1934-3647).
[0242] All plasmid isolations were conducted according to
manufacturer's instructions using HiSpeed Maxi Kit
(Quiagen/Germany). For this purpose, Escherichia coli XL10 cells
(Stratagene) containing the final expression plasmid or one of the
interim plasmids were cultivated in Luria-Bertoni (LB) medium
(Sigma-Aldrich) containing 50 mg/ml Ampicillin (Sigma-Aldrich).
[0243] For isolation of tef1 promoter sequence (SEQ ID NO: 17), 50
.mu.l PCR reaction contained 1.25 U PfuUltra Hotstart Mastermix
(Stratagene) and 1.25 U Taq PCR Mastermix (Quiagen), 22 .mu.l
H.sub.2O, 22.1 pmol of forward primer TefP_forw (XbaI) (SEQ ID NO:
21) and 100 pmol of reverse primer TefP_rev (SpeI) (SEQ ID NO: 22),
and 100 ng of template (genomic DNA of Schizophyllum commune). The
reaction was carried out in Gene Amp.RTM. PCR System 9700 Thermal
Cycler from PE Applied Biosystems. The following program was used
for the amplification: initial heating step up to 95.degree. C. for
4 minutes was followed by 30 cycles of 30 seconds denaturing at
95.degree. C., 30 seconds of annealing step at 55.degree. C., 1
minute elongation step at 72.degree. C., followed by one cycle at
72.degree. C. for 10 minutes.
[0244] For amplification of the synthetic .beta.-1,3-glucan
synthase gene (SEQ ID NO: 1), 50 .mu.l PCR reaction contained 1.25
U PfuUltra Hotstart Mastermix (Stratagene) and 1.25 U Taq PCR
Mastermix (Quiagen), 22 .mu.l H.sub.2O, 100 pmol of forward primer
GS1_forw (SpeI) (SEQ ID NO: 27) and 22 pmol of reverse primer
GS1_rev (SalI)(SEQ ID NO: 28), 100 ng template (pMK_GS_1). The
reaction was carried out in Gene Amp.RTM. PCR System 9700 Thermal
Cycler from PE Applied Biosystems. The following program was used
for the amplification: an initial heating step up to 95.degree. C.
for 4 minutes was followed by 30 cycles of 30 seconds denaturing at
95.degree. C., 30 seconds of annealing step at 55.degree. C., 8
minutes elongation step at 72.degree. C., followed by one cycle at
72.degree. C. for 10 minutes.
[0245] In the next PCR reaction step, fusion of the first two PCR
products (tef1 promoter (SEQ ID NO: 17) with .beta.-1,3-glucan
synthase gene (SEQ ID NO: 1) was carried out. 50 .mu.l PCR reaction
contained 1.25 U of Pwo Hotstart Mastermix (Roche) and 1.25 U Taq
PCR Mastermix (Quiagen), 22 .mu.l of H.sub.2O, 22.1 pmol of each
primer: Fusion TefP_GS1_forw (XbaI) (SEQ ID NO: 29) and Fusion
TefP_GS1_rev (SalI) (SEQ ID NO: 30) and 100 ng of both templates.
The reaction was carried out in Gene Amp.RTM. PCR System 9700
Thermal Cycler from PE Applied Biosystems. The following program
was used for the fusion of both sequences: an initial heating step
up to 95.degree. C. for 4 minutes was followed by 30 cycles of 30
seconds denaturing at 95.degree. C., 30 seconds of annealing step
at 55.degree. C., 8 minutes elongation step at 72.degree. C.,
followed by one cycle at 72.degree. C. for 10 minutes.
[0246] The product of the fusion PCR was treated with SalI and XbaI
restriction enzymes (Roche) according to manufacturer's
instructions and the vector (pBluescript 2KSP, Stratagene Cloning
Systems) was linearized using the same restriction enzymes and
subsequently treated with alkaline phosphatase (Roche) according to
manufacturer's instructions. Both, the digested PCR product and the
linearized pBluescript 2KSP vector, were ligated using T4 DNA
Ligase (New England Biolabs, Inc., Beverly, Mass./USA) and
transformed into Escherichia coli XL10 cells (Stratagene) according
to manufacturer's instructions.
[0247] For isolation of tef1 terminator sequence (SEQ ID NO: 18)
following PCR reaction was carried out: 50 .mu.l PCR reaction
contained 1.25 U of Pwo Hotstart Mastermix (Roche) and 1.25 U Taq
PCR Mastermix (Quiagen), 22 .mu.l of H.sub.2O, 24 pmol of forward
primer TefT_forw (SalI) (SEQ ID NO: 23) and 21 pmol of reverse
primer TefT_rev (SalI) (SEQ ID NO: 24), and 100 ng of template
(genomic DNA of Schizophyllum commune). The reaction was carried
out in Gene Amp.RTM. PCR System 9700 Thermal Cycler from PE Applied
Biosystems. The following program was used: an initial heating step
up to 95.degree. C. for 4 minutes was followed by 30 cycles of 30
seconds denaturing at 95.degree. C., 30 seconds of annealing step
at 60.degree. C., 1 minute elongation step at 72.degree. C.,
followed by one cycle at 72.degree. C. for 10 minutes. The PCR
product was treated with SalI restriction enzyme (Roche) and
ligated with the plasmid containing tef1 promoter and
.beta.-1,3-glucan synthase, which was before linearized with SalI
restriction enzyme (Roche) and treated with alkaline phosphatase
(Roche) according to manufacturer's instructions. After ligation,
the DNA construct was transformed into Escherichia coli XL10 cells
(Stratagene) according to manufacturer's instructions. To enable
screening of Schizophyllum commune strains after transformation
with the .beta.-1,3-glucan synthase expression, a plasmid selection
marker (ura1; SEQ ID NO: 33) was introduced into the plasmid. For
that purpose, ura1 gene was isolated from the genomic DNA of
Schizophyllum commune. The PCR reaction contained 2.5 U of Pwo
Hotstart Mastermix (Roche), 22 .mu.l of H.sub.2O, 21 pmol of
forward primer Ura_forw (NotI) (SEQ ID NO: 19), 38 pmol of reverse
primer Ura_rev (XbaI) (SEQ ID NO: 20) and 100 ng of the template
(genomic DNA of Schizophyllum commune). The reaction was carried
out in Gene Amp.RTM. PCR System 9700 Thermal Cycler from PE Applied
Biosystems. The following program was used: an initial heating step
up to 95.degree. C. for 4 minutes was followed by 30 cycles of 30
seconds denaturing at 95.degree. C., 30 seconds of annealing step
at 60.degree. C., 2 minutes elongation step at 72.degree. C.,
followed by one cycle at 72.degree. C. for 10 minutes. The PCR
Product was digested with XbaI and NotI restriction enzymes (Roche)
and ligated into the XbaI/NotI site of the .beta.-1,3-glucan
synthase expression plasmid (pGS_1) using T4 DNA Ligase (New
England Biolabs, Inc., Beverly, Mass./USA). The resulting plasmid
encoding .beta.-1,3-glucan synthase with tef1 promoter and
terminator, and containing ura1 selection marker was transformed
into Escherichia coli XL10 cells (Stratagene) according to
manufacturer's instructions.
[0248] For the transformation of Schizophyllum commune with the
.beta.-1,3-glucan synthase expression plasmid (pGS_1), plasmid
preparation was carried out as follows. Escherichia coli XL10 cells
containing the .beta.-1,3-glucan synthase expression plasmid were
cultivated in Luria-Bertoni (LB) medium (Sigma-Aldrich) containing
50 mg/ml Ampicillin (Sigma-Aldrich) and the plasmid isolation was
conducted according to manufacturer's instructions using HiSpeed
Maxi Kit (Quiagen).
[0249] Schizophyllum commune (Lu15527; obtained from strain
collection of University of Jena (Germany), Prof. E. Kothe, Jena
University internal strain name: 12-43) was transformed based on
the method described by van Peer et al. (van Peer, loc cit) as
basis. The method was modified according to the description
below.
[0250] For preparation of S. commune protoplasts, fresh culture was
inoculated on a plate containing complex medium (CYM). For
incubation at 26.degree. C. for 2-3 days, plates were sealed with
parafilm.
[0251] For inoculation of liquid preculture (50 ml working volume),
the biomass from the plate was macerated for 1 minute at 13500 rpm
using T 25 digital ULTRA-TURRAX.RTM. (IKA), inoculated in shaking
flask containing liquid CYM medium and incubated at 30.degree. C.,
220 rpm for further 3 days. Main culture was inoculated with 15 ml
of the preculture in 200 ml CYM medium and incubated further 3 days
at 30.degree. C. at 220 rpm. After finishing the culture growth,
the main culture was divided in four 50 ml samples and centrifuged
(4000 rpm, 15 min). Obtained pellet was washed twice with 1 M
MgSO.sub.4 (50 ml) (Roth). After washing, four samples were united
and dissolved 50 ml 1M MgSO.sub.4.
[0252] To enable cell wall lysis, 100 mg Caylase (Cayla, Toulouse,
France) were dissolved in 1 mL 1 M MgSO.sub.4 and added to the
pellet suspension. The sample was incubated over night at
30.degree. C. under slight shaking (70 rpm). Subsequently distilled
water was added to the sample (in 1:1 ratio), which was then
incubated under slight shaking (70 rpm) for further 5 min. After
this step, cells were incubated without shaking for 10 min and
subsequently centrifuged (1100 rpm, 20 min, 4.degree. C.). After
the supernatant was filtrated using Miracloth-Membrane, one volume
of cold 1 M sorbitol was added and the sample was allowed to
equilibrate for 10 min. Subsequently, the sample was centrifuged
(2000 rpm, 20 min, 2.degree. C.). Pellet was washed by
re-suspending carefully in 1 M sorbitol and centrifugation step was
repeated. Finally the protoplasts were re-suspended in 1 M sorbitol
and 50 mM CaCl.sub.2 at a concentration of 10.sup.8 protoplasts per
ml.
[0253] DNA used for transformation was a circular plasmid (pGS_1)
and the integration in the genome of S. commune was ectopic. To
transform the protoplasts with the DNA, 100 .mu.l protoplasts and
10 .mu.l DNA (5-10 .mu.g) were gently mixed and incubated for 60
min on ice. Subsequently, one volume of PEG 4000 (40%) was added
and the sample was incubated for 5 to 10 min on ice. After adding
2.5 ml regeneration medium (complete medium containing 0.1 .mu.g/ml
Phleomycin and 0.5 M MgSO.sub.4), the sample was incubated at
30.degree. C., 70 rpm overnight.
[0254] After PEG mediated transformation, regenerated protoplasts
were spread on petri dishes containing 40 ml solidified minimal
medium: 2 g aspartic acid (Roth), 20 g glucose (Sigma), 0.5 g
MgSO.sub.4 (Roth), 0.5 g KH.sub.2PO.sub.4, 1 g K.sub.2HPO.sub.4
(both from Riedel-de Haen), 120 .mu.g thiaminhydrochlorid (Roth)
per liter, pH 6.3 containing 1% low melting agarose (Sigma).
Selection plates were incubated 5 days at 30.degree. C.
Example 2
Cloning of the .beta.-1,3-Glucan Synthase Expression Plasmid [pGS
2] and Transformation into S. commune
[0255] The expression plasmid for the second .beta.-1,3-glucan
synthase (SEQ ID NO: 3) (pGS_2) was prepared analogously to the
preparation of (pGS_1) as described above in Example 1.
[0256] As a source of the promoter sequence tef1 (SEQ ID NO: 17);
the same PCR product as in Example 1 was used.
[0257] Polynucleotide represented by SEQ ID NO: 3 was amplified
from the (pMK_GS_2) plasmid following PCR reaction: 50 .mu.l PCR
reaction contained 1.25 U PfuUltra Hotstart Mastermix (Stratagene)
and 1.25 U Taq PCR Mastermix (Quiagen), 22 .mu.l H.sub.2O, 23 pmol
of each primer: GS2_forw (SpeI)/SEQ ID NO: 31) and GS2_rev
(EcoRV)(SEQ ID NO: 32), 100 ng of template (pMK_GS_2). The reaction
was carried out in Gene Amp.RTM. PCR System 9700 Thermal Cycler
from PE Applied Biosystems. The following program was used for the
amplification: an initial heating step up to 95.degree. C. for 4
minutes was followed by 30 cycles of 30 seconds denaturing at
95.degree. C., 30 seconds of annealing step at 53.degree. C., 8
minutes elongation step at 72.degree. C., followed by one cycle at
72.degree. C. for 10 minutes.
[0258] For isolation of tef1 terminator sequence (SEQ ID NO: 18)
and introduction of the EcoRV (5') and ApaI (3') sites, the
following PCR reaction was carried out: 50 .mu.l PCR reaction
contained 1.25 U of Pwo Hotstart Mastermix (Roche) and 1.25 U Taq
PCR Mastermix (Quiagen), 22 .mu.l of H.sub.2O, 37 pmol of forward
primer TefT_forw (EcoRV) (SEQ ID NO: 25) and 25 pmol of reverse
primer TefT_rev (ApaI)(SEQ ID NO: 26), and 100 ng of template
(genomic DNA of Schizophyllum commune). The reaction was carried
out in Gene Amp.RTM. PCR System 9700 Thermal Cycler from PE Applied
Biosystems. The following program was used: an initial heating step
up to 95.degree. C. for 4 minutes was followed by 30 cycles of 30
seconds denaturing at 95.degree. C., 30 seconds of annealing step
at 58.degree. C., 1 minute elongation step at 72.degree. C.,
followed by one cycle at 72.degree. C. for 10 minutes. The PCR
product was treated with EcoRV and ApaI restriction enzyme (Roche)
and ligated with the vector (pBluescript 2KSP, Stratagene Cloning
Systems), which was before digested the same restriction enzymes.
After ligation, the DNA construct was transformed into Escherichia
coli XL10 cells (Stratagene), according to manufacturer's
instructions.
[0259] Subsequently, tef1 promoter was cloned into the plasmid. For
this purpose, the PCR product was digested with XbaI and SpeI
(Roche) and ligated with the plasmid described above according to
manufacturer's instructions, containing tef1 terminator which was
linearized using XbaI and SpeI. The ligation was carried out as
described in Example 1 herein. After ligation, the DNA construct
was transformed into Escherichia coli XL10 cells (Stratagene)
according to manufacturer's instructions.
[0260] Subsequently, ura1 was cloned into the plasmid. The same PCR
product as in Example 1 was used. After digestion of the PCR
product with NotI and XbaI, the fragment was cloned into the
plasmid carrying the polynucleotide represented by SEQ ID NO: 7,
tef1 promoter and terminator sequences. Before ligation, the
plasmid was linearized by NotI and XbaI. Transformation was carried
out as described above in Example 1.
[0261] Finally, .beta.-1,3-glucan synthase (SEQ ID NO: 3) was
ligated into the plasmid. For this purpose, the PCR product was
treated with SpeI and EcoRV and ligated into the target expression
plasmid as described above. Transformation was carried out as
described above in Example 1.
[0262] Transformation of Schizophyllum commune with (pGS_2)
followed as described in Example 1.
Example 3
Verification of the Functionality of the Engineered S. commune
Strains
[0263] Genetically modified S. commune strains generated as
described above were tested in shaking flasks for increased
schizophyllan production. To assure the reproducibility of the
results, a three-step cultivation was applied, consisting of two
pre-cultures and one main culture as further described herein
below.
[0264] For the cultivation of the genetically modified
Schizophyllum commune strains, two different media were used. For
cultivation on solid media, CYM medium (25 g agar (Difco), 20 g
glucose (Sigma), 2 g trypticase peptone (Roth), 2 g yeast extract
(Difco), 0.5 g MgSO.sub.4.times.7 H.sub.2O (Roth), 0.5 g
KH.sub.2PO.sub.4 and 1 g K.sub.2HPO.sub.4 (both from Riedel-de
Haen) per liter H.sub.2O) was used. Strains were inoculated on agar
plates containing CYM medium covered with cellophane (to avoid
mycelium growth into the agar) and incubated for three to four days
at 26.degree. C.
[0265] For the liquid cultures, the following medium was used
(hereinafter referred to as "Standard Medium"): 30 g glucose
(Sigma), 3 g yeast extract (Difco), 1 g KH.sub.2PO.sub.4 (Riedel-de
Haen), 0.5 g MgSO.sub.4.times.7 H.sub.2O (Roth) per liter
H.sub.2O.
[0266] For both pre-cultures and for main culture, 250 ml shaking
flasks filled with 30 ml Standard Medium were used. The cultivation
was carried out at 27.degree. C. and 225 rpm. Before each
inoculation, the biomass was homogenized for 1 minute at 13500 rpm
using T 25 digital ULTRA-TURRAX.RTM. (IKA).
[0267] The first pre-culture was inoculated with 50 mg of wet
biomass. The cultures were incubated for 72 hours. After 72 hours,
the second pre-culture was started. The concentration of the
homogenized wet biomass from the first pre-culture used for
inoculation was 250 mg. Cultivation time was 45 hours. After 45
hours, the main culture was inoculated with 500 mg of homogenized
wet biomass from the second pre-culture and cultivated for another
45 hours.
[0268] After the cultivation was finished, standard analytical
methods as described herein below were applied to define the
biomass concentration, schizophyllan concentration, ethanol
concentration and residual glucose in medium. 50 ml aliquots of the
cultures were stabilized with 3 g/l Acticide BW20 (Thor).
[0269] Ethanol and glucose concentration was estimated using HPLC
method. For this purpose 14 ml of the culture were centrifuged (30
min, 8500 rpm). The supernatant was sterile-filtrated and 1 ml of
the filtrate was injected for the HPLC analysis (HPLC cation
exchanger: Aminex HPX-87-H, BIO-RAD with 0.5 M H.sub.2SO.sub.4,
Roth, as eluent and 0.5 ml/min flow rate at 30.degree. C.).
[0270] Due to the fact that schizophyllan consists only of glucose
molecules, the quantification of this polymer can be done using
standard analytical methods for glucose. 10 ml of the culture, 20
ml H.sub.2O and 90 .mu.l Acticide BW20 were mixed. The sample was
digested for 24 h at 40.degree. C. with .beta.-glucanase (0.3 ml)
(Erbsloh). After the incubation, the sample was centrifuged (30
minutes at 3400 g) and the supernatant was analyzed for glucose and
ethanol content using HPLC cation exchanger (Aminex HPX-87-H,
BIO-RAD) with 0.5 M H.sub.2SO.sub.4 (Roth) as eluent and 0.5 ml/min
flow rate at 30.degree. C.
[0271] For the biomass determination, the remaining biomass in form
of pellet (after .beta.-glucanase digestion sample was centrifuged)
was washed twice with 50 ml H.sub.2O, filtrated using
Whatman-Filter (with determination of filter's weight before
filtration), washed twice with H.sub.2O and dried in HB43S drying
scale from Mettler Toledo. Drying of the filter was carried out for
5 to 10 minutes at 180.degree. C. Subsequently, weight of the dry
filter was determined.
[0272] The evaluation of the results obtained in shaking flasks
showed clear effect of the overexpression of both .beta.-1,3-glucan
synthases on the schizophyllan production. Because of the fact that
in the expression plasmid was ectopically integrated into genome
and the integration locus has an explicit effect on the expression
of the target gene, 40 clones carrying the plasmid (pGS_1) and 40
clones carrying the plasmid (pGS_2) were tested in shaking flask
experiments. The increase of schizophyllan production in the
genetically modified strains is shown in Table 1 in comparison to
the non-modified Schizophyllum commune control strain. The results
shown in the Table 1 refer to the best strain of each 40 strains
tested. For classification of the strains, the amount of
schizophyllan in the sample was decisive. 10 ml of the culture, 20
ml H.sub.2O and 90 .mu.l Acticide BW20 were mixed. The sample was
digested for 24 h at 40.degree. C. with 0.3 ml .beta.-glucanase
(Erbsloh). After the incubation, the sample was centrifuged (30
minutes at 3400 g) and the supernatant was analyzed for glucose and
ethanol content using HPLC cation exchanger (Aminex HPX-87-H,
BIO-RAD) with 0.5 M H.sub.2SO.sub.4 (Roth) as eluent and 0.5 ml/min
flow rate at 30.degree. C.
[0273] In addition to increased yields of schizophyllan production
in the genetically modified S. commune strains, a clear decrease in
the synthesis of the by-product ethanol was observed. This can be
an indication that the excess rate of glucose by up-regulated
.beta.-1,3-glucan synthase activity is metabolized more directly in
the schizophyllan pathway instead of partly being used for ethanol
synthesis.
TABLE-US-00001 TABLE 1 Comparison of Schizophyllum commune control
strain with two genetically modified S. commune strains carrying
glucan synthase expression plasmid (pGS_1) or (pGS_2).
Schizophyllan EtOH [% Strain [%] [%] S. commune control strain 100
100 S. commune (pGS_1) 220 9 S. commune (pGS_2) 215 3.6
Structure and Conformation Analysis of the Product
[0274] To assure that the polymer synthesized through genetically
modified S. commune strains is schizophyllan, XRD and NMR methods
were applied to confirm the structure of the molecule as
follows.
[0275] Powder X-ray diffraction (XRD) allows rapid, non-destructive
analysis of materials consisting of multiple components. Moreover,
the sample preparation is straightforward. The data from the
measurement is presented as a diffractogram in which the diffracted
intensity (I) is shown as a function of scattering angle 2.theta..
The crystallinity of the given material can be determined by this
measurement. In general, crystalline materials have reflection
patterns of a series of sharp peaks whereas amorphous materials
give a broad signals. Many polymers exhibit semicrystalline
behaviour which can also be detected by XRD (Hammond, The basics of
chrystallography and diffraction, 3.sup.rd Ed., Oxford University
Press 2009).
Sample Preparation from Aqueous Solution
[0276] Aqueous solution containing schizophyllan was poured in
ethanol to precipitate schizophyllan. The precipitation was
filtered and dried either in a vacuum oven. The dried sample was
measured by XRD.
Sample Measurement and Results by XRD
[0277] Schizophyllan exhibits a triple helical structure. This was
evident from the diffractogram of the precipitated and dried
schizophyllan sample (FIG. 2). The triple helix could be seen as an
intensive diffraction at 5.degree. 2.theta. and the amorphous
region of the material gives broad diffraction in the range of
20-25.degree. 2.theta. (Hisamatsu, Carbohydr Res (1997), 298:
117).
Sample Measurement and Results by NMR
[0278] The NMR spectra were recorded on a Varian VNMRS 600 MHz
system equipped with a .sup.13C-enhanced cryo probe (inverse
configuration) at ambient temperatures or at 50.degree. C. using
standard pulse sequences for .sup.1H and .sup.13C.
[0279] It is known that schizophyllan has a triple helical
structure formed by three .beta.(1-3)-D-glucan chains held together
by hydrogen bonds in water. This structure is shielded in the
magnetic field due to the rigid, ordered conformation. This means
that in NMR spectrum, chemical shifts for schizophyllan are not
obtained (Rinaudo, Carbohydr Polym (1982), 2: 135; Vlachou,
Carbohydr Polym (2001), 46: 349) (2D NMR). In order to investigate
the molecular structure of schizophyllan and not the macromolecular
structure consisting of triple helices and further to record the
successful NMR spectra with a good signal-to-noise ratio, the
conformation of the triple helix has to be changed. It is also
known that the triple helix of schizophyllan can be altered to form
a random coil structure by addition of DMSO. When the DMSO
concentration exceeds a certain threshold values (i.e. 87%), the
conformation change takes place; therefore deuterated
[D.sub.6]-DMSO was used as a solvent for the measurements. This
conformation matter is important to take into consideration when
conducting NMR experiments for schizophyllan. Hence, the sample was
measured in [D.sub.6]-DMSO, the well-resolved spectra can be
obtained (FIGS. 2 and 3).
SUMMARY
[0280] The chemical structures of the materials from S. commune
(GS_1) and S. commune (GS_2) strain was identified to be the
correct for that of schizophyllan. In addition, the materials
exhibit the triple helix conformations.
Sequences Referred to in the Present Application
TABLE-US-00002 [0281] TABLE 2 Assignment of SEQ ID NOs. SEQ ID NO:
type of sequence description 1 nucleotide sequence Gene sequence*
1,3-.beta.-D-glucan synthase I of S. commune strain Lu15531 2 amino
acid sequence translation of SEQ ID NO: 5 3 nucleotide sequence
Gene sequence* 1,3-.beta.-D-glucan synthase II of S. commune strain
Lu15531 4 amino acid sequence translation of SEQ ID NO: 7 5
nucleotide sequence cDNA 1,3-.beta.-D-glucan synthase I of S.
commune strain Lu 15531 6 amino acid sequence polypeptide sequence
1,3-.beta.-D-glucan synthase I of S. commune strain Lu15531 7
nucleotide sequence cDNA 1,3-.beta.-D-glucan synthase II of S.
commune strain Lu 15531 8 amino acid sequence polypeptide sequence
1,3-.beta.-D-glucan synthase II of S. commune strain Lu15531 9
nucleotide sequence Gene sequence* 1,3-.beta.-D-glucan synthase I
of S. commune strain Lu 15634 10 amino acid sequence translation of
SEQ ID NO: 13 11 nucleotide sequence Gene sequence*
1,3-.beta.-D-glucan synthase II of S. commune strain Lu 15634 12
amino acid sequence translation of SEQ ID NO: 15 13 nucleotide
sequence cDNA 1,3-.beta.-D-glucan synthase I of S. commune strain
Lu 15634 14 amino acid sequence polypeptide sequence
1,3-.beta.-D-glucan synthase I of S. commune strain Lu 15634 15
nucleotide sequence cDNA 1,3-.beta.-D-glucan synthase II of S.
commune strain Lu 15634 16 amino acid sequence polypeptide sequence
1,3-.beta.-D-glucan synthase II of S. commune strain Lu 15634 17
nucleotide sequence tef1 promoter from S. commune 18 nucleotide
sequence tef1 terminator from S. commune 19 nucleotide sequence
Ura_forw (Notl) primer 20 nucleotide sequence Una_rev (Xbal) primer
21 nucleotide sequence TefP_forw (Xbal) primer 22 nucleotide
sequence TefP_rev (Spel) primer 23 nucleotide sequence TefT_forw
(Sall) primer 24 nucleotide sequence TefT_rev (Sall) primer 25
nucleotide sequence TefT_forw (EcoRV) primer 26 nucleotide sequence
TefT_rev (Apal) primer 27 nucleotide sequence GS1_forw (Spel)
primer 28 nucleotide sequence GS1_rev(Sall) primer 29 nucleotide
sequence Fusion_TefP_GS1_forw (Xbal) primer 30 nucleotide sequence
Fusion_TefP GS1 rev (Sall) primer 31 nucleotide sequence GS2_forw
(Spel) primer 32 nucleotide sequence GS2_rev (EcoRV) primer 33
nucleotide sequence ura gene (S. commune) 34 amino acid sequence
Ura protein *Gene sequence includes introns and flanking regions.
In the gene sequences below (for SEQ ID NO: 1, 3, 9 and 11),
predicted exons are shown in capital letters, introns are shown in
lower case letters.
TABLE-US-00003 Gene sequence 1,3-.beta.-D-glucan synthase I of S.
commune strain Lu15531 DNA S. commune SEQ ID NO: 1
CCCGTCCCTCAAGGCCGTTCTTTCGCTGGCGACCGACCCGGTGTTCGCGAGAA
CCTGTTGTTTCTGACGATCATCAGCCCTTTCTTCTCGTCGCTCTTTAGCTCTCCC
TAGACCGTCTTTTACTCTACTCTTCGACGCACGCCATGTCCGGCCCAGGATATG
GCAGGAATCCATTCGACAATCCCCCGCCCAACAGAGGTCCCTATGGCCAGCAG
CCAGGTTTCCCGGGGCCCGGCCCTCGGCCTTACGACTCGGACGCGGACATGA
GCCAGACCTATGGCAGCACAACCAGGCTCGCCGGCAGTGCCGGTTACAGCGA
CAGAAACGgtgcgcacgtcgctaccgtacttcctcgatcgtcgattcacataccatgcagGCA
GCTTCGACGGCGACCGCTCCTACGCGCCCTCAATTGACTCGCGCGCCAGCGTGCCCAGCAT
ATCGCCCTTCGCAGACCCGGGTATCGGCTCTAATGAGCCGTATCCCGCTTGGT
CGGTCGAACGCCAGATTCCCATGTCCACGGAGGAGATTGAGGACATCTTCCTC
GACCTCACCCAAAAGTTTGGCTTCCAGCGCGACTCCATGCGGAATACGgtgcgtga
ataagcagcccactcgaccgcgggaacagcacaattgacctgtcacccagTTCGACTTCAT
GATGCACCTCCTCGATTCCCGTGCCTCGCGCATGACGCCCAACCAAGCTCTGCTCACGCTT
CACGCCGACTACATTGGTGGCCAGCATGCCAATTACCGGAAGTGGTATTTCGCC
GCACAGCTCAACCTCGATGACGCGGTCGGGCAAACCAATAACCCCGGTATCCA
GCGCTTGAAGACCATCAAGGGCGCTACGAAGACCAAGTCGCTCGACAGCGCAC
TCAACCGCTGGCGCAACGCGATGAACAACATGAGCCAGTACGATCGCCTCCGG
CAAATTGCGCTCTACCTCCTCTGCTGGGGTGAAGCAGGCAACATCCGTCTG GC
GCCCGAGTGCTTGTGCTTCATCTTCAAGTGCGCGGACGACTACTACAGAAGTCC
CGAGTGTCAGAACCGGATGGACCCCGTGCCGGAAGGGCTGTACCTGCAGACG
GTCATCAAGCCGCTCTATCGCTTCCTACGTGATCAGGCGTACGAAGTCGTTGAT
GGGAAGCAAGTGAAGCGCGAGAAGGACCACGACCAGATTATCGGTTATGACGA
CGTCAACCAGTTATTCTGGTATCCGGAAGGTTTGGCTAAGATCGTCATGTCGGA
CAACgtgcgtatgatcttatcggttaaaattcgtccgctcacatctttccagACACGACTTG
TAGATGTACCTCCGGCGCAGCGGTTCATGAAGTTCGCCAAGATCGAGTGGAACCGCGTCTTC
TTCAAGACGTACTTTGAGAAGCGCTCTACTGCCCATCTCCTGGTCAACTTCAAC
CGTATATGGATCCTCCACGTCTCGATGTACTTCTTCTACACGGCATTCAACTCTC
CACGAGTCTACGCGCCGCACGGCAAACTCGACCCCTCCCCTGAGATGACCTGG
TCCGCGACTGCCCTTGGAGGCGCTGTGTCCACCATGATCATGATCCTTGCCACT
ATCGCGGAGTACACCTACATCCCCACGACATGGAACAATGCGTCGCACCTCAC
CACGCGGCTCATTTTCCTCCTGGTCATCCTCGCGCTCACTGCTGGCCCAACATT
CTATATCGCCATGATAGACGGACGCACGGACATCGGCCAAGTACCACTCATCGT
GGCCATAGTGCAGTTCTTCATCTCCGTCGTCGCCACCCTCGCTTTCGCTACCAT
CCCTTCTGGTCGCATGTTCGGCGACCGTGTGGCTGGCAAGTCAAGAAAGCACA
TGGCATCGCAGACGTTCACAGCGTCGTACCCGTCCATGAAGCGGTCATCTCGC
GTAGCGAGTATCATGCTGTGGCTTTTGGTCTTTGGCTGCAAATACGTCGAGTCT
TACTTCTTCTTGACGTCCTCCTTCTCCAGCCCGATCGCGGTCATGGCGCGTACG
AAGGTACAGGGCTGCAACGACCGTATCTTCGGCAGCCAGCTGTGCACGAATCA
GGTCCCGTTCGCGCTGGCAATCATGTACGTGATGGACCTGGTACTGTTCTTCCT
GGACACGTACCTGTGGTACATCATCTGGCTGGTGATCTTCTCGATGGTGCGCG
CGTTCAAGCTTGGTATCTCGATCTGGACGCCCTGGAGCGAGATCTTCACCCGCA
TGCCGAAGCGTATTTACGCAAAGCTGCTGGCGACGGCCGAGATGGAGGTCAAG
TATAAGCCCAAGgtatgctgaattcaatctggtcaggtgaattcaccctcatattgtggtaca
gGTGCTCGTCTCACAAATCTGGAACGCGGTCATCATCTCCATGTACCGGGAGCATCTCTTGTC
CATCGAGCACGTCCAGCGCTTGCTTTACCACCAGGTTGATGGTCCCGATGGCC
GCCGCACCCTCAGGGCACCGCCGTTCTTCACCAGCCAGCGAACTGCGAAGCCA
GGCCTGTTCTTCCCTCCTGGTGGCGAGGCTGAGCGCCGCATCTCGTTCTTTGC
CTCATCGCTGACGACCGCGCTCCCGGAGCCTCTGCCGATCGACGCCATGCCCA
CCTTCACCGTGCTCGTTCCCCATTACTCCGAGAAGATTCTGCTCAGTCTGCGCG
AGATTATCCGCGAGGAGGACCAGAACACCCGCGTTACCTTACTGGAGTACCTCA
AGCAGCTCCACCCTGTCGAATGGGACAATTTCGTCAAGGACACCAAGATCTTGG
CGGAAGAGTCGGGAGACGTCCAGGACGAGAAGCGCGCGCGCACGGACGACTT
GCCGTTCTATTGCATCGGGTTCAAGACCTCGTCACCAGAGTACACCCTGCGTAC
GCGTATCTGGGCCTCACTGCGCGCACAGACGCTGTACCGCACGGTCTCCGGTA
TGATGAACTACTCCAAGGCGATTAAGCTCCTCTATCGCGTCGAGAACCCGGATG
TCGTTCATGCCTTCGGTGGGAACACGGAACGTCTTGAACGCGAGCTTGAGCGC
ATGTCTCGCCGCAAGTTCAAGTTCGTCATCTCGATGCAGCGGTACTCCAAGTTC
AACAAGGAGGAGCAGGAGAACGCCGAGTTCCTTCTGCGCGCGTACCCGGATTT
GCAGATCGCGTACCTCGATGAAGAGCCCGGTCCCAGCAAGAGCGACGAGGTTC
GGTTGTTTTCGACACTCATCGACGGACACTCCGAGGTGGACGAGAAGACGGGC
CGCCGCAAGCCCAAGTTCCGCATCGAGCTGCCCGGTAACCCCATCCTCGGTGA
CGGGAAGTCGGATAACCAGAACCACGCCATCGTCTTCTACCGCGGCGAGTACA
TTCAGGTCATTGACGCTAACCAGGACAATTACCTGGAAGAGTGTCTCAAGATCC
GTAATGTCCTGGGCGAGTTTGAGGAATACTCCGTGTCGAGCCAGAGCCCGTAC
GCGCAGTGGGGCCACAAGGAGTTCAACAAGTGCCCCGTCGCTATCCTGGGTTC
CCGCGAGTACATCTTCTCGGAGAACATCGGTATCCTCGGTGACATCGCTGCCG
GCAAGGAACAGACGTTCGGTACCATTACGGCGCGTGCGCTTGCGTGGATCGGC
GGCAAGCTGCATTACGGTCACCCGGATTTCCTCAATGCGACGTTCATGACGACG
CGTGGTGGCGTGTCAAAAGCGCAGAAGGGCTTGCATCTTAACGAGGATATCTTC
GCTGGTATGACCGCCGTGTCCCGCGGAGGGCGCATCAAGCACATGGAGTACTA
CCAGTGCGGCAAAGGTCGTGATCTCGGATTCGGCACGATCTTGAACTTCCAGA
CCAAGATCGGTACTGGTATGGGCGAGCAGCTGCTCTCGCGCGAGTACTACTAT
CTGGGCACGCAATTGCCTATCGACCGGTTCTTGACGTTCTACTACGCGCACG CT
GGTTTCCATGTCAACAACATCCTGGTCATCTACTCCATCCAGGTCTTCATGGTCA
CCCgtaagtgcaggccctcatgaccgccgagcaagcagtctaacggatgtgcagTGCTGTACC
TGGGCACATTGAACAAGCAGCTGTTCATCTGCAAGGTCAACTCCAATGGCCAGGTTCTT
AGTGGACAAGCTGGGTGCTACAACCTCATCCCGGTCTTCGAGTGGATTCGCCG
GAGTATCATCTCCATCTTCTTGGTGTTCTTCATCGCCTTCTTGCCGTTGTTCTTG
CAAGgtatgttcacttctcatgtgccatttgtcaatcgctcactcgtacgacagAGCTTTGCG
AACGCGGAACAGGAAAGGCGTTGCTGCGTCTCGGGAAGCACTTCCTGTCACTGTCGCCCAT
CTTCGAAGTGTTCTCCACCCAAATCTACTCGCAGGCGCTCTTGAACAACATGAG
TTTCGGTGGTGCGCGCTACATCGCTACAGGACGCGGTTTCGCGACGAGTCGGA
TACCCTTCAACATCCTCTACTCGCGTTTCGCGCCGCCGAGCATCTACATGGGCA
TGCGTAATCTGCTGCTCTTGCTGTACGCGACGATGGCCATTTGGATCCCACACC
TGATCTACTTCTGGTTCTCCGTCCTCTCCCTCTGCATCGCGCCATTCATGTTCAA
TCCGCATCAATTCTCGTACGCTGACTTCATCATCGACTACCGGGAGTTCTTGCG
CTGGATGTCGCGCGGTAACTCGCGGACGAAGGCGAGTAGCTGGTACGGATATT
GCCGTCTGTCGCGTACCGCGATTACTGGGTACAAGAAGAAGAAACTGGGACAC
CCGTCGGAGAAGCTGTCGGGCGATGTGCCGCGTGCGCCGTGGAGGAACGTCA
TCTTCTCGGAGATCCTTTGGCCCATCGGCGCGTGCATCATCTTCATCGTCGCGT
ACATGTTCGTCAAATCGTTCCCTGACGAGCAGGGCAACGCGCCGCCGAGCCCG
CTGGTCCGCATTCTGCTCATCGCGGTTGGCCCTACTGTGTGGAACGCGGCGGT
GCTCATCACGCTGTTCTTCCTGTCGCTCTTCCTGGGCCCGATGATGGATGGCTG
GGTCAAGTTCGGCTCAGTCATGGCGGCACTTGCGCATGGTCTAGCGCTCATAG
GCATGCTCACGTTCTTCGAGTTCTTCgtacgtccttcgcgttgttgtggtcgagtgctttgct
aacaccgccttcagTGGTTCCTCGAGCTCTGGGATGCCTCGCACGCCGTGCTCGGCGTCATC
GCCATTATTGCCGTTCAGCGCGGGATCCAGAAGATCCTCATTGCCGTCTTCCTG
ACGCGTGAGTACAAGCACGACGAGACGAACCGCGCGTGGTGGACAGGTAAATG
GTATGGACGCGGGCTGGGTACCTCGGCCATGTCCCAGCCGGCGCGCGAGTTC
ATCGTGAAGATCGTGGAGATGTCGCTGTGGACGTCGGACTTCCTGCTTGCGCA
CCTGTTGCTCATCATCTTGACGGTGCCGCTACTGCTGCCGTTCTTCAACTCGAT
CCATTCGACGATGCTTTgtgagtgatttgtagtcgttggtcacggatgattgctgactcgcg
tgcagTCTGGTTGCGCCCTTCGAAGCAGATTAGGCAACCTCTGTTCTCCACTAAGCAGAAGCG
GCAACGGCGATGGATTgtaagttcctttgattgctctggctaccgaccttcgctcacctgtct
cagGTCATGAAGTATACCGTGGTATATCTCGTGGTGGTGGCTTTCCTCGTTGCGCTCATCGCT
CTGCgtacgttttctgtcgcgctcaccctctattttcactaacgtttcctccagCCGCGCTCT
TCCGCGAGAGCATCCACTTCAACTGCGAGATCTGCCAGAGTATATAGTCATATAACGACGTCTA
TCGTATCGCCGGACGAGAGCCCCGTCGCCTACACACTGACATGGAATTGCTGT
GTATACAATCGATCTTCTGACCGCGTCGGGGGCGTTGCCGTCTTTCTACTATCA
ACTTGCTTGTGTATCAACATTTCTTCTCTCCAAGCCTACATTGACATAGAGTAATA
GCCCATGTTCATACAACAATCGCATAGCATTGCATATACCAT Translation of SEQ ID
NO: 5 amino acid S. commune SEQ ID NO: 2
MSGPGYGRNPFDNPPPNRGPYGQQPGFPGPGPRPYDSDADMSQTYGSTTRLAG
SAGYSRNGSFDGDRSYAPSIDSRASVPSISPFADPGIGSNEPYPAWSVERQIPMS
TEEIEDIFLDLTQKFGFQRDSMRNTFDFMMHLLDSRASRMTPNQALLTLHADYIGGQ
HANYRKWYFAAQLNLDDAVGQTNNPGIQRLKTIKGATKTKSLDSALNRWRNAMNN
MSQYDRLRQIALYLLCWGEAGNIRLAPECLCFIFKCADDYYRSPECQNRMDPVPEG
LYLQTVIKPLYRFLRDQAYEVVDGKQVKREKDHDQIIGYDDVNQLFVVYPEGLAKIVM
SDNTRLVDVPPAQRFMKFAKIEWNRVFFKTYFEKRSTAHLLVNFNRIWILHVSMYFF
YTAFNSPRVYAPHGKLDPSPEMTWSATALGGAVSTMIMILATIAEYTYIPTTWNNAS
HLTTRLIFLLVILALTAGPTFYIAMIDGRTDIGQVPLIVAIVQFFISVVATLAFATIPSGRM
FGDRVAGKSRKHMASQTFTASYPSMKRSSRVASIMLWLLVFGCKYVESYFFLTSSF
SSPIAVMARTKVQGCNDRIFGSQLCTNQVPFALAIMYVMDLVLFFLDTYLWYIIWLVI
FSMVRAFKLGISIWTPWSEIFTRMPKRIYAKLLATAEMEVKYKPKVLVSQIWNAVIISM
YREHLLSIEHVQRLLYHQVDGPDGRRTLRAPPFFTSQRTAKPGLFFPPGGEAERRIS
FFASSLTTALPEPLPIDAMPTFTVLVPHYSEKILLSLREIIREEDQNTRVTLLEYLKQLH
PVEWDNFVKDTKILAEESGDVQDEKRARTDDLPFYCIGFKTSSPEYTLRTRIWASLR
AQTLYRTVSGMMNYSKAIKLLYRVENPDVVHAFGGNTERLERELERMSRRKFKFVI
SMQRYSKFNKEEQENAEFLLRAYPDLQIAYLDEEPGPSKSDEVRLFSTLIDGHSEVD
EKTGRRKPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYIQVIDANQDNYLEECLKIR
NVLGEFEEYSVSSQSPYAQWGHKEFNKCPVAILGSREYIFSENIGILGDIAAGKEQTF
GTITARALAWIGGKLHYGHPDFLNATFMTTRGGVSKAQKGLHLNEDIFAGMTAVSR
GGRIKHMEYYQCGKGRDLGFGTILNFQTKIGTGMGEQLLSREYYYLGTQLPIDRFLT
FYYAHAGFHVNNILVIYSIQVFMVTLLYLGTLNKQLFICKVNSNGQVLSGQAGCYNLI
PVFEWIRRSIISIFLVFFIAFLPLFLQELCERGTGKALLRLGKHFLSLSPIFEVFSTQIYS
QALLNNMSFGGARYIATGRGFATSRIPFNILYSRFAPPSIYMGMRNLLLLLYATMAIW
IPHLIYFWFSVLSLCIAPFMFNPHQFSYADFIIDYREFLRWMSRGNSRTKASSWYGY
CRLSRTAITGYKKKKLGHPSEKLSGDVPRAPWRNVIFSEILWPIGACIIFIVAYMFVKS
FPDEQGNAPPSPLVRILLIAVGPTVWNAAVLITLFFLSLFLGPMMDGVVVKFGSVMAA
LAHGLALIGMLTFFEFFWFLELWDASHAVLGVIAIIAVQRGIQKILIAVFLTREYKHDET
NRAVWVTGKWYGRGLGTSAMSQPAREFIVKIVEMSLWTSDFLLAHLLLIILTVPLLLP
FFNSIHSTMLFWLRPSKQIRQPLFSTKQKRQRRWIVMKYTVVYLVVVAFLVALIALPA
LFRESIHFNCEICQSI Gene sequence 1,3-.beta.-D-glucan synthase II of
S. commune strain Lu15531 DNA S. commune SEQ ID NO: 3
CTGTCCAAAGAAGAGATCGAGGACATCTTCCTCGATCTGACGCAGAAGTTTGGC
TTTCAGCGGGATTCCATGCGGAACATGgtacgtggcgtatgcccatgtgcggcgttctgagg
cctaaacgttttccgccagTTCGACTTCACCATGCAGCTGCTTGACAGCCGAGCGTCTCGTATG
ACCCCCAACCAGGCGCTCCTCACCCTCCACGCCGACTACATTGGTGGCCAGCA
TGCGAACTACCGGAAGTGGTACTTCGCGGCGCAGCTCGACCTTGACGACGCCG
TGGGACAAACTCAGAATCCGGGTCTCAACCGCCTCAAGTCCACTCGCGGATCG
GGCAAGCGACCACGCCATGAAAAGTCGCTGAACACGGCATTGGAGCGCTGGC
GGCAAGCCATGAACAACATGTCGCAGTATGACCGCTTACGCCAGATCGCGCTC
TACCTGCTCTGCTGGGGCGAAGCGGCGCAAGTGCGATTCATGCCCGAGTGCTT
GTGCTTCATCTTCAAGTGCGCCGACGACTATTATCGTTCGCCGGAGTGCCAGAA
CAGGATGGAGCCGGTACCGGAGGGTCTCTACCTGAGGACGGTCGTAAAGCCG
CTCTACAGATTTGTCCGGGATCAAGGCTATGAGGTGGTGGAGGGAAAATTCGTA
CGGCGGGAACGGGATCACGACCAAATCATTGGTTACGATGACGTGAATCAGCT
GTTCTGGTACCCGGAGGGCATTGCCCGTATCGTCCTGTCGGACAAGgtaagcacctc
tgtgcatcttctgtgacatacagggctaattgtcgagcagAGTCGTCTGGTCGACCTCCCTC
CAGCACAGCGCTTCATGAAGTTCGACCGTATCGAGTGGAATCGCGTCTTCTTCAAGACG
TTCTACGAGACTCGATCCTTTACGCATCTTTTGGTCGACTTCAACCGTATCTGGG
TCGTGCACATCGCTCTCTACTTCTTCTACACCGCATACAACTCCCCCACGATCTA
CGCCATCAACGGCAACACTCCGACGTCTCTGGCTTGGAGCGCGACTGCGCTCG
GCGGTGCGGTAGCGACAGGTATCATGATCCTCGCCACGATCGCCGAGTTCTCG
CACATCCCCACGACATGGAACAACACCTCGCATCTGACTCGCCGCCTCGCCTTC
CTCCTCGTCACGCTCGGCCTCACATGTGGTCCGACGTTCTACGTCGCGATTGCA
GAGAGCAACGGGAGCGGCGGCTCTTTGGCCTTGATTCTCGGCATCGTCCAGTT
CTTCATCTCCGTCGTAGCGACTGCGCTCTTCACTATCATGCCTTCTGGTCGTAT
GTTCGGCGACCGCGTCGCAGGCAAGAGTCGCAAGTATCTCGCCAGCCAGACGT
TCACGGCCAGCTACCCGTCGTTGCCCAAGCACCAGCGGTTCGCATCACTCCTG
ATGTGGTTCCTCATCTTCGGGTGCAAGTTGACGGAGAGTTACTTCTTCCTGACG
TTGTCCTTCCGCGACCCTATTCGCGTCATGGTCGGCATGAAGATCCAGAACTGC
GAGGACAAGATTTTCGGCAGCGGCCTTTGCAGGAATCACGCAGCATTCACCCT
CACGATCATGTACATCATGGACCTCGTCTTGTTCTTCCTCGACACCTTCCTTTGG
TATGTCATCTGGAACTCGGTTTTCAGTATCGCACGCTCTTTCGTACTCGGCCTTT
CGATCTGGACACCATGGAGGGACATCTTCCAGCGTCTGCCGAAGCGTATCTAC
GCGAAGCTTCTAGCGACCGGCGACATGGAGGTCAAGTACAAGCCCAAGgtgtgtga
atagctcgctgtaaggttcttgattctgactcattcgcagGTCTTGGTTTCGCAAATCTGG
AACGCCATCATCATCTCCATGTACCGCGAGCACTTGCTCTCTATCGAGCACGTTCAAAAGC
TCCTGTACCATCAAGTGGACACTGGCGAAGCCGGCAAGCGGAGTCTTCGCGCG
CCTCCGTTCTTCGTCGCGCAGGGCAGCAGCGGTGGCTCGGGCGAGTTCTTCCC
GCCTGGTAGCGAGGCTGAGCGTCGTATCTCTTTCTTCGCGCAGTCTCTATCTAC
GGAGATTCCTCAGCCCATCCCGGTTGACGCCATGCCGACGTTCACAGTGCTTA
CGCCTCACTACAGCGAGAAGgtgcgctttttcctgggcgcattcaacattagctgactgtcgt
gcacagATCCTTCTTTCGCTCCGTGAGATTATCCGCGAGGAGGACCAGAACACCCGCGTG
ACATTGCTTGAGTATCTCAAGCAGCTTCACCCGGTCGAGTGGGAGAACTTCGTC
AAGGACACCAAGATTTTGGCCGAGGAGTCCGCTATGTTCAACGGTCCAAGTCCT
TTCGGCAACGATGAGAAGGGTCAGTCCAAGATGGACGATCTTCCTTTCTACTGC
ATCGGTTTCAAGAGCGCCGCGCCCGAGTACACCCTCCGCACCCGTATCTGGGC
GTCCTTGCGCGCGCAGACCCTCTACCGCACGGTCTCCGGCATGATGAACTATG
CGAAGGCGATTAAGCTGCTCTACCGCGTCGAGAACCCCGAGGTCGTGCAGCAG
TTCGGCGGTAACACGGACAAGCTCGAGCGCGAGTTGGAGCGGATGGCCCGGC
GGAAGTTCAAGTTCCTGGTGTCCATGCAGCGCTACTCGAAGTTCAACAAGGAGG
AGCACGAGAACGCCGAGTTCTTGCTCCGCGCGTACCCGGACCTGCAGATCGCG
TACCTGGAGGAAGAGCCTCCTCGCAAGGAGGGTGGCGATCCACGCATCTTCTC
TGCCCTCGTCGACGGCCACAGCGACATCATCCCGGAGACCGGCAAGCGGCGC
CCCAAGTTCCGCATCGAGCTGCCCGGCAACCCCATTCTCGGTGACGGCAAGTC
GGACAACCAGAACCACGCCATCGTCTTCTACCGCGGCGAGTACCTCCAGCTTAT
CGACGCCAACCAGGACAACTACCTCGAGGAGTGCTTGAAGATCCGTAACGTAC
TCGCCGAGTTCGAGGAGTACGACGTCTCTAGCCAGAGTCCGTACGCGCAGTGG
AGTGTCAAGGAGTTCAAGCGCTCCCCGGTCGCCATCGTCGGTGCACGCGAGTA
TATCTTCTCGGAGCACATCGGTATTCTCGGTGATTTGGCGGCTGGCAAGGAACA
GACGTTCGGTACGCTCACGGCACGCAACAACGCCTTCCTTGGCGGCAAGCTGC
ACTACGGTCACCCGGATTTCCTCAACGCCCTCTACATGAACACGCGCGGTGGT
GTCTCCAAGGCGCAGAAGGGTCTCCATCTCAACGAGGATATTTACGCCGGTATG
AACGCGGTCGGTCGCGGTGGACGCATCAAGCATAGCGAATACTACCAGTGCGG
CAAGGGTCGTGACCTCGGTTTTGGCACCATCTTGAACTTCCAGACCAAGATCGG
TACGGGTATGGGCGAGCAGATCCTCTCGCGCGAGTACTACTACCTCGGAACCC
AATTGCCCATCGATCGCTTCCTCACGTTCTACTACGCGCACCCAGGTTTCCAGA
TCAACAACATGCTGGTTATCCTATCCGTGCAGGTCTTCATCGTTACCAgtacgttgatt
gcatatcgttagcctgacagcgtctgacgaattcccagTGGTCTTCCTCGGTACCTTGAAGTCT
TCGGTCACGATCTGCAAGTACACGTCCAGCGGTCAGTACATCGGTGGTCAATCCG
GTTGCTACAACCTCGTCCCGGTCTTCCAGTGGATCGAGCGCTGCATCATCAGCA
TCTTCTTGGTGTTCATGATCGCTTTCATGCCGCTCTTCCTGCAAGgtaagagctcgtca
acctgctcaagggccttgcgctgatcatcatctcagAACTCGTCGAGCGCGGTACCTGGAGTGCC
ATCTGGCGTCTGCTCAAGCAGTTTATGTCGCTGTCGCCTGTCTTCGAGGTGTTC
TCCACCCAGATTCAGACACACTCCGTGTTGAGCAACTTGACGTTCGGTGGTGCG
CGTTACATCGCTACCGGTCGTGGGTTCGCCACCAGTCGTATCAGCTTCAGCATC
TTGTTCTCGCGTTTCGCAGGCCCGAGTATCTACCTCGGCATGCGCACGCTCATT
ATGCTGCTCTACGTGACGTTGACGATCTGGACGCCATGGGTCATTTACTTCTGG
GTTTCCATTCTCTCGCTCTGCATCGCGCCGTTCTTGTTCAATCCGCATCAATTCG
TCTTCTCGGATTTCCTCATCGACTACAGgtacgtcggacgagcgctgttccgcgacgtaagctg
accggttatacagGGAATACCTCCGGTGGATGTCGCGTGGTAACTCGCGCTCGCACAAC
AACTCCTGGATTGGGTACTGCCGGTTGTCCCGCACGATGATCACTGGGTACAA
GAAGAAGAAGCTGGGCCACCCGTCGGAGAAGCTTTCCGGCGACGTTCCTCGTG
CAGGCTGGCGCGCCGTCTTATTCTCGGAGATCATCTTCCCGGCATGCATGGCC
ATCCTCTTCATCATCGCGTACATGTTCGTCAAGTCGTTCCCTCTCGACGGCAAG
CAGCCTCCCTCCGGCCTCGTTCGCATCGCCGTCGTGTCTATCGGCCCCATCGT
GTGGAACGCCGCCATCCTGTTGACGCTCTTCCTTGTGTCGTTGTTCCTCGGCCC
CATGCTCGACCCGGTCTTCCCCCTCTTCGGTTCCGTTATGGCCTTCATCGCGCA
TTTCCTCGGCACAATCGGAATGATTGGGTTCTTCGAGTTCCTGgtatgtgcccataccttt
cattcgtcttcaactatctaacagattcatagTGGTTCCTCGAGTCCTGGGAGGCGTCGCATGCC
GTGCTGGGTCTCATCGCCGTCATCTCCATCCAGCGCGCCATTCACAAAATTCTT
ATCGCCGTTTTCCTCAGTCGCGAGTTCAAGCACGACGAGACGAACAGGGCTTG
GTGGACTGGTCGCTGGTATGGCCGTGGCCTCGGCACGCACGCCATGTCGCAG
CCGGCGCGTGAGTTCGTCGTCAAGATCATCGAGTTGTCGCTCTGGAGCTCGGA
TCTCATACTCGGCCACATCCTGCTGTTCATGCTTACTCCGGCTGTCCTCATCCC
GTACTTCGACCGTCTGCACGCCATGATGCTCTgtacgtcgtgtctcattgtttgtgttggtc
atactcttaccctctcttagTCTGGCTGCGCCCCTCAAAGCAAATCCGCGCGCCTCTGTACTCA
ATCAAGCAGAAGAGGCAAAGACGCTGGATTgtcagtgttcagtgccttattctatcagctcttac
tgacgtcttcatagATCATGAAGTACGGTACTGTATACGTTACCGTCATCGCGATCTTCGTCG
CGCTCATCGCGCTTCgtgagtacccttgctatctttcgtacctgagcgtcgctgacccctttc
ccagCCCTCGTCTTCCGACACACTCTAAAGGTCGAGTGCTCCCTTTGCGACAGCTTGTAATAT
CGGACTCGTATATATCTAGACTTCTCCGCACCATGTGTAGCTGACGCTTGGGTA
TACTTCGCGGTGCCGAGCTAATTGTCGACGGACATTCTCCATCGTTGAGTGCAG
CGACATCGGGTGGTTTACGACACGGACACTTTTCATTGTACCCTCTACGAATGC
AAGAACTCTCTTACGACCAGTACCTATGTGCTAAGCCGTCGCCTGTTCAGGATC
ATACATACATACGTTTCTAGATACCTTACAGTTAGGCCTATTCAGGGAGAGTCTG CATAAAA
Translation of SEQ ID NO: 7 amino acid S. commune SEQ ID NO: 4
MRNMFDFTMQLLDSRASRMTPNQALLTLHADYIGGQHANYRKVVYFAAQLDLDDAV
GQTQNGLNRLKSTRGSGKRPRHEKSLNTALERWRQAMNNMSQYDRLRQIALYLL
CWGEAAQVRFMPECLCFIFKCADDYYRSPECQNRMEPVPEGLYLRTVVKPLYRFV
RDQGYEVVEGKFVRRERDHDQIIGYDDVNQLFWYPEGIARIVLSDKSRLVDLPPAQ
RFMKFDRIEWNRVFFKTFYETRSFTHLLVDFNRIWVVHIALYFFYTAYNSPTIYAING
NTPTSLAWSATALGGAVATGIMILATIAEFSHIPTTWNNTSHLTRRLAFLLVTLGLTCG
PTFYVAIAESNGSGGSLALILGIVQFFISVVATALFTIMPSGRMFGDRVAGKSRKYLA
SQTFTASYPSLPKHQRFASLLMWFLIFGCKLTESYFFLTLSFRDPIRVMVGMKIQNC
EDKIFGSGLCRNHAAFTLTIMYIMDLVLFFLDTFLVVYVIWNSVFSIARSFVLGLSIWTP
WRDIFQRLPKRIYAKLLATGDMEVKYKPKVLVSQIWNAIIISMYREHLLSIEHVQKLLY
HQVDTGEAGKRSLRAPPFFVAQGSSGGSGEFFPPGSEAERRISFFAQSLSTEIPQPI
PVDAMPTFTVLTPHYSEKILLSLREIIREEDQNTRVTLLEYLKQLHPVEWENFVKDTKI
LAEESAMFNGPSPFGNDEKGQSKMDDLPFYCIGFKSAAPEYTLRTRIWASLRAQTL
YRTVSGMMNYAKAIKLLYRVENPEVVQQFGGNTDKLERELERMARRKFKFLVSMQ
RYSKFNKEEHENAEFLLRAYPDLQIAYLEEEPPRKEGGDPRIFSALVDGHSDIIPETG
KRRPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYLQLIDANQDNYLEECLKIRNVLA
EFEEYDVSSQSPYAQWSVKEFKRSPVAIVGAREYIFSEHIGILGDLAAGKEQTFGTL
TARNNAFLGGKLHYGHPDFLNALYMNTRGGVSKAQKGLHLNEDIYAGMNAVGRGG
RIKHSEYYQCGKGRDLGFGTILNFQTKIGTGMGEQILSREYYYLGTQLPIDRFLTFYY
AHPGFQINNMLVILSVQVFIVTMVFLGTLKSSVTICKYTSSGQYIGGQSGCYNLVPVF
QWIERCIISIFLVFMIAFMPLFLQELVERGTWSAIWRLLKQFMSLSPVFEVFSTQIQTH
SVLSNLTFGGARYIATGRGFATSRISFSILFSRFAGPSIYLGMRTLIMLLYVTLTIWTP
VVVIYFVVVSILSLCIAPFLFNPHQFVFSDFLIDYREYLRWMSRGNSRSHNNSWIGYCR
LSRTMITGYKKKKLGHPSEKLSGDVPRAGWRAVLFSEIIFPACMAILFIIAYMFVKSFP
LDGKQPPSGLVRIAWSIGPIVWNAAILLTLFLVSLFLGPMLDPVFPLFGSVMAFIAHF
LGTIGMIGFFEFLWFLESWEASHAVLGLIAVISIQRAIHKILIAVFLSREFKHDETNRAW
WTGRVVYGRGLGTHAMSQPAREFVVKIIELSLWSSDLILGHILLFMLTPAVLIPYFDRL
HAMMLFWLRPSKQIRAPLYSIKQKRQRRWIIMKYGTVYVTVIAIFVALIALPLVFRHTL
KVECSLCDSL cDNA 1,3-.beta.-D-glucan synthase I of S. commune strain
Lu15531 DNA S. commune SEQ ID NO: 5
ATGTCCGGCCCAGGATATGGCAGGAATCCATTCGACAATCCCCCGCCCAACAG
AGGTCCCTATGGCCAGCAGCCAGGTTTCCCGGGGCCCGGCCCTCGGCCTTAC
GACTCGGACGCGGACATGAGCCAGACCTATGGCAGCACAACCAGGCTCGCCG
GCAGTGCCGGTTACAGCGACAGAAACGGCAGCTTCGACGGCGACCGCTCCTAC
GCGCCCTCAATTGACTCGCGCGCCAGCGTGCCCAGCATATCGCCCTTCGCAGA
CCCGGGTATCGGCTCTAATGAGCCGTATCCCGCTTGGTCGGTCGAACGCCAGA
TTCCCATGTCCACGGAGGAGATTGAGGACATCTTCCTCGACCTCACCCAAAAGT
TTGGCTTCCAGCGCGACTCCATGCGGAATACGTTCGACTTCATGATGCACCTCC
TCGATTCCCGTGCCTCGCGCATGACGCCCAACCAAGCTCTGCTCACGCTTCAC
GCCGACTACATTGGTGGCCAGCATGCCAATTACCGGAAGTGGTATTTCGCCGC
ACAGCTCAACCTCGATGACGCGGTCGGGCAAACCAATAACCCCGGTATCCAGC
GCTTGAAGACCATCAAGGGCGCTACGAAGACCAAGTCGCTCGACAGCGCACTC
AACCGCTGGCGCAACGCGATGAACAACATGAGCCAGTACGATCGCCTCCGGCA
AATTGCGCTCTACCTCCTCTGCTGGGGTGAAGCAGGCAACATCCGTCTGGCGC
CCGAGTGCTTGTGCTTCATCTTCAAGTGCGCGGACGACTACTACAGAAGTCCCG
AGTGTCAGAACCGGATGGACCCCGTGCCGGAAGGGCTGTACCTGCAGACGGT
CATCAAGCCGCTCTATCGCTTCCTACGTGATCAGGCGTACGAAGTCGTTGATGG
GAAGCAAGTGAAGCGCGAGAAGGACCACGACCAGATTATCGGTTATGACGACG
TCAACCAGTTATTCTGGTATCCGGAAGGTTTGGCTAAGATCGTCATGTCGGACA
ACACACGACTTGTAGATGTACCTCCGGCGCAGCGGTTCATGAAGTTCGCCAAGA
TCGAGTGGAACCGCGTCTTCTTCAAGACGTACTTTGAGAAGCGCTCTACTGCCC
ATCTCCTGGTCAACTTCAACCGTATATGGATCCTCCACGTCTCGATGTACTTCTT
CTACACGGCATTCAACTCTCCACGAGTCTACGCGCCGCACGGCAAACTCGACC
CCTCCCCTGAGATGACCTGGTCCGCGACTGCCCTTGGAGGCGCTGTGTCCACC
ATGATCATGATCCTTGCCACTATCGCGGAGTACACCTACATCCCCACGACATGG
AACAATGCGTCGCACCTCACCACGCGGCTCATTTTCCTCCTGGTCATCCTCGCG
CTCACTGCTGGCCCAACATTCTATATCGCCATGATAGACGGACGCACGGACATC
GGCCAAGTACCACTCATCGTGGCCATAGTGCAGTTCTTCATCTCCGTCGTCGCC
ACCCTCGCTTTCGCTACCATCCCTTCTGGTCGCATGTTCGGCGACCGTGTGGCT
GGCAAGTCAAGAAAGCACATGGCATCGCAGACGTTCACAGCGTCGTACCCGTC
CATGAAGCGGTCATCTCGCGTAGCGAGTATCATGCTGTGGCTTTTGGTCTTTGG
CTGCAAATACGTCGAGTCTTACTTCTTCTTGACGTCCTCCTTCTCCAGCCCGATC
GCGGTCATGGCGCGTACGAAGGTACAGGGCTGCAACGACCGTATCTTCGGCAG
CCAGCTGTGCACGAATCAGGTCCCGTTCGCGCTGGCAATCATGTACGTGATGG
ACCTGGTACTGTTCTTCCTGGACACGTACCTGTGGTACATCATCTGGCTGGTGA
TCTTCTCGATGGTGCGCGCGTTCAAGCTTGGTATCTCGATCTGGACGCCCTGGA
GCGAGATCTTCACCCGCATGCCGAAGCGTATTTACGCAAAGCTGCTGGCGACG
GCCGAGATGGAGGTCAAGTATAAGCCCAAGGTGCTCGTCTCACAAATCTGGAA
CGCGGTCATCATCTCCATGTACCGGGAGCATCTCTTGTCCATCGAGCACGTCCA
GCGCTTGCTTTACCACCAGGTTGATGGTCCCGATGGCCGCCGCACCCTCAGGG
CACCGCCGTTCTTCACCAGCCAGCGAACTGCGAAGCCAGGCCTGTTCTTCCCT
CCTGGTGGCGAGGCTGAGCGCCGCATCTCGTTCTTTGCCTCATCGCTGACGAC
CGCGCTCCCGGAGCCTCTGCCGATCGACGCCATGCCCACCTTCACCGTGCTCG
TTCCCCATTACTCCGAGAAGATTCTGCTCAGTCTGCGCGAGATTATCCGCGAGG
AGGACCAGAACACCCGCGTTACCTTACTGGAGTACCTCAAGCAGCTCCACCCT
GTCGAATGGGACAATTTCGTCAAGGACACCAAGATCTTGGCGGAAGAGTCGGG
AGACGTCCAGGACGAGAAGCGCGCGCGCACGGACGACTTGCCGTTCTATTGCA
TCGGGTTCAAGACCTCGTCACCAGAGTACACCCTGCGTACGCGTATCTGGGCC
TCACTGCGCGCACAGACGCTGTACCGCACGGTCTCCGGTATGATGAACTACTC
CAAGGCGATTAAGCTCCTCTATCGCGTCGAGAACCCGGATGTCGTTCATGCCTT
CGGTGGGAACACGGAACGTCTTGAACGCGAGCTTGAGCGCATGTCTCGCCGCA
AGTTCAAGTTCGTCATCTCGATGCAGCGGTACTCCAAGTTCAACAAGGAGGAGC
AGGAGAACGCCGAGTTCCTTCTGCGCGCGTACCCGGATTTGCAGATCGCGTAC
CTCGATGAAGAGCCCGGTCCCAGCAAGAGCGACGAGGTTCGGTTGTTTTCGAC
ACTCATCGACGGACACTCCGAGGTGGACGAGAAGACGGGCCGCCGCAAGCCC
AAGTTCCGCATCGAGCTGCCCGGTAACCCCATCCTCGGTGACGGGAAGTCGGA
TAACCAGAACCACGCCATCGTCTTCTACCGCGGCGAGTACATTCAGGTCATTGA
CGCTAACCAGGACAATTACCTGGAAGAGTGTCTCAAGATCCGTAATGTCCTGGG
CGAGTTTGAGGAATACTCCGTGTCGAGCCAGAGCCCGTACGCGCAGTGGGGCC
ACAAGGAGTTCAACAAGTGCCCCGTCGCTATCCTGGGTTCCCGCGAGTACATCT
TCTCGGAGAACATCGGTATCCTCGGTGACATCGCTGCCGGCAAGGAACAGACG
TTCGGTACCATTACGGCGCGTGCGCTTGCGTGGATCGGCGGCAAGCTGCATTA
CGGTCACCCGGATTTCCTCAATGCGACGTTCATGACGACGCGTGGTGGCGTGT
CAAAAGCGCAGAAGGGCTTGCATCTTAACGAGGATATCTTCGCTGGTATGACCG
CCGTGTCCCGCGGAGGGCGCATCAAGCACATGGAGTACTACCAGTGCGGCAAA
GGTCGTGATCTCGGATTCGGCACGATCTTGAACTTCCAGACCAAGATCGGTACT
GGTATGGGCGAGCAGCTGCTCTCGCGCGAGTACTACTATCTGGGCACGCAATT
GCCTATCGACCGGTTCTTGACGTTCTACTACGCGCACGCTGGTTTCCATGTCAA
CAACATCCTGGTCATCTACTCCATCCAGGTCTTCATGGTCACCCTGCTGTACCT
GGGCACATTGAACAAGCAGCTGTTCATCTGCAAGGTCAACTCCAATGGCCAGGT
TCTTAGTGGACAAGCTGGGTGCTACAACCTCATCCCGGTCTTCGAGTGGATTCG
CCGGAGTATCATCTCCATCTTCTTGGTGTTCTTCATCGCCTTCTTGCCGTTGTTC
TTGCAAGAGCTTTGCGAACGCGGAACAGGAAAGGCGTTGCTGCGTCTCGGGAA
GCACTTCCTGTCACTGTCGCCCATCTTCGAAGTGTTCTCCACCCAAATCTACTC
GCAGGCGCTCTTGAACAACATGAGTTTCGGTGGTGCGCGCTACATCGCTACAG
GACGCGGTTTCGCGACGAGTCGGATACCCTTCAACATCCTCTACTCGCGTTTCG
CGCCGCCGAGCATCTACATGGGCATGCGTAATCTGCTGCTCTTGCTGTACGCG
ACGATGGCCATTTGGATCCCACACCTGATCTACTTCTGGTTCTCCGTCCTCTCC
CTCTGCATCGCGCCATTCATGTTCAATCCGCATCAATTCTCGTACGCTGACTTCA
TCATCGACTACCGGGAGTTCTTGCGCTGGATGTCGCGCGGTAACTCGCGGACG
AAGGCGAGTAGCTGGTACGGATATTGCCGTCTGTCGCGTACCGCGATTACTGG
GTACAAGAAGAAGAAACTGGGACACCCGTCGGAGAAGCTGTCGGGCGATGTGC
CGCGTGCGCCGTGGAGGAACGTCATCTTCTCGGAGATCCTTTGGCCCATCGGC
GCGTGCATCATCTTCATCGTCGCGTACATGTTCGTCAAATCGTTCCCTGACGAG
CAGGGCAACGCGCCGCCGAGCCCGCTGGTCCGCATTCTGCTCATCGCGGTTG
GCCCTACTGTGTGGAACGCGGCGGTGCTCATCACGCTGTTCTTCCTGTCGCTCT
TCCTGGGCCCGATGATGGATGGCTGGGTCAAGTTCGGCTCAGTCATGGCGGCA
CTTGCGCATGGTCTAGCGCTCATAGGCATGCTCACGTTCTTCGAGTTCTTCTGG
TTCCTCGAGCTCTGGGATGCCTCGCACGCCGTGCTCGGCGTCATCGCCATTATT
GCCGTTCAGCGCGGGATCCAGAAGATCCTCATTGCCGTCTTCCTGACGCGTGA
GTACAAGCACGACGAGACGAACCGCGCGTGGTGGACAGGTAAATGGTATGGAC
GCGGGCTGGGTACCTCGGCCATGTCCCAGCCGGCGCGCGAGTTCATCGTGAA
GATCGTGGAGATGTCGCTGTGGACGTCGGACTTCCTGCTTGCGCACCTGTTGC
TCATCATCTTGACGGTGCCGCTACTGCTGCCGTTCTTCAACTCGATCCATTCGA
CGATGCTTTTCTGGTTGCGCCCTTCGAAGCAGATTAGGCAACCTCTGTTCTCCA
CTAAGCAGAAGCGGCAACGGCGATGGATTGTCATGAAGTATACCGTGGTATATC
TCGTGGTGGTGGCTTTCCTCGTTGCGCTCATCGCTCTGCCCGCGCTCTTCCGC
GAGAGCATCCACTTCAACTGCGAGATCTGCCAGAGTATATAG polypeptide sequence
1,3-.beta.-D-glucan synthase I of S. commune strain Lu15531 amino
acid S. commune SEQ ID NO: 6
MSGPGYGRNPFDNPPPNRGPYGQQPGFPGPGPRPYDSDADMSQTYGSTTRLAG
SAGYSDRNGSFDGDRSYAPSIDSRASVPSISPFADPGIGSNEPYPAWSVERQIPMS
TEEIEDIFLDLTQKFGFQRDSMRNTFDFMMHLLDSRASRMTPNQALLTLHADYIGGQ
HANYRKWYFAAQLNLDDAVGQTNNPGIQRLKTIKGATKTKSLDSALNRWRNAMNN
MSQYDRLRQIALYLLCWGEAGNIRLAPECLCFIFKCADDYYRSPECQNRMDPVPEG
LYLQTVIKPLYRFLRDQAYEVVDGKQVKREKDHDQIIGYDDVNQLFWYPEGLAKIVM
SDNTRLVDVPPAQRFMKFAKIEWNRVFFKTYFEKRSTAHLLVNFNRIWILHVSMYFF
YTAFNSPRVYAPHGKLDPSPEMTWSATALGGAVSTMIMILATIAEYTYIPTTWNNAS
HLTTRLIFLLVILALTAGPTFYIAMIDGRTDIGQVPLIVAIVQFFISVVATLAFATIPSGRM
FGDRVAGKSRKHMASQTFTASYPSMKRSSRVASIMLWLLVFGCKYVESYFFLTSSF
SSPIAVMARTKVQGCNDRIFGSQLCTNQVPFALAIMYVMDLVLFFLDTYLWYIIWLVI
FSMVRAFKLGISIWTPWSEIFTRMPKRIYAKLLATAEMEVKYKPKVLVSQIWNAVIISM
YREHLLSIEHVQRLLYHQVDGPDGRRTLRAPPFFTSQRTAKPGLFFPPGGEAERRIS
FFASSLTTALPEPLPIDAMPTFTVLVPHYSEKILLSLREIIREEDQNTRVTLLEYLKQLH
PVEWDNFVKDTKILAEESGDVQDEKRARTDDLPFYCIGFKTSSPEYTLRTRIWASLR
AQTLYRTVSGMMNYSKAIKLLYRVENPDVVHAFGGNTERLERELERMSRRKFKFVI
SMQRYSKFNKEEQENAEFLLRAYPDLQIAYLDEEPGPSKSDEVRLFSTLIDGHSEVD
EKTGRRKPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYIQVIDANQDNYLEECLKIR
NVLGEFEEYSVSSQSPYAQWGHKEFNKCPVAILGSREYIFSENIGILGDIAAGKEQTF
GTITARALAWIGGKLHYGHPDFLNATFMTTRGGVSKAQKGLHLNEDIFAGMTAVSR
GGRIKHMEYYQCGKGRDLGFGTILNFQTKIGTGMGEQLLSREYYYLGTQLPIDRFLT
FYYAHAGFHVNNILVIYSIQVFMVTLLYLGTLNKQLFICKVNSNGQVLSGQAGCYNLI
PVFEWIRRSIISIFLVFFIAFLPLFLQELCERGTGKALLRLGKHFLSLSPIFEVFSTQIYS
QALLNNMSFGGARYIATGRGFATSRIPFNILYSRFAPPSIYMGMRNLLLLLYATMAIW
IPHLIYFWFSVLSLCIAPFMFNPHQFSYADFIIDYREFLRWMSRGNSRTKASSWYGY
CRLSRTAITGYKKKKLGHPSEKLSGDVPRAPWRNVIFSEILWPIGACIIFIVAYMFVKS
FPDEQGNAPPSPLVRILLIAVGPTVWNAAVLITLFFLSLFLGPMMDGVVVKFGSVMAA
LAHGLALIGMLTFFEFFWFLELWDASHAVLGVIAIIAVQRGIQKILIAVFLTREYKHDET
NRAVWVTGKWYGRGLGTSAMSQPAREFIVKIVEMSLWTSDFLLAHLLLIILTVPLLLP
FFNSIHSTMLFWLRPSKQIRQPLFSTKQKRQRRWIVMKYTVVYLVVVAFLVALIALPA
LFRESIHFNCEICQSI cDNA 1,3-.beta.-D-glucan synthase II of S. commune
strain Lu15531 DNA S. commune SEQ ID NO: 7
ATGCGGAACATGTTCGACTTCACCATGCAGCTGCTTGACAGCCGAGCGTCTCGT
ATGACCCCCAACCAGGCGCTCCTCACCCTCCACGCCGACTACATTGGTGGCCA
GCATGCGAACTACCGGAAGTGGTACTTCGCGGCGCAGCTCGACCTTGACGACG
CCGTGGGACAAACTCAGAATCCGGGTCTCAACCGCCTCAAGTCCACTCGCGGA
TCGGGCAAGCGACCACGCCATGAAAAGTCGCTGAACACGGCATTGGAGCGCTG
GCGGCAAGCCATGAACAACATGTCGCAGTATGACCGCTTACGCCAGATCGCGC
TCTACCTGCTCTGCTGGGGCGAAGCGGCGCAAGTGCGATTCATGCCCGAGTGC
TTGTGCTTCATCTTCAAGTGCGCCGACGACTATTATCGTTCGCCGGAGTGCCAG
AACAGGATGGAGCCGGTACCGGAGGGTCTCTACCTGAGGACGGTCGTAAAGCC
GCTCTACAGATTTGTCCGGGATCAAGGCTATGAGGTGGTGGAGGGAAAATTCGT
ACGGCGGGAACGGGATCACGACCAAATCATTGGTTACGATGACGTGAATCAGC
TGTTCTGGTACCCGGAGGGCATTGCCCGTATCGTCCTGTCGGACAAGAGTCGT
CTGGTCGACCTCCCTCCAGCACAGCGCTTCATGAAGTTCGACCGTATCGAGTG
GAATCGCGTCTTCTTCAAGACGTTCTACGAGACTCGATCCTTTACGCATCTTTTG
GTCGACTTCAACCGTATCTGGGTCGTGCACATCGCTCTCTACTTCTTCTACACC
GCATACAACTCCCCCACGATCTACGCCATCAACGGCAACACTCCGACGTCTCTG
GCTTGGAGCGCGACTGCGCTCGGCGGTGCGGTAGCGACAGGTATCATGATCCT
CGCCACGATCGCCGAGTTCTCGCACATCCCCACGACATGGAACAACACCTCGC
ATCTGACTCGCCGCCTCGCCTTCCTCCTCGTCACGCTCGGCCTCACATGTGGTC
CGACGTTCTACGTCGCGATTGCAGAGAGCAACGGGAGCGGCGGCTCTTTGGCC
TTGATTCTCGGCATCGTCCAGTTCTTCATCTCCGTCGTAGCGACTGCGCTCTTC
ACTATCATGCCTTCTGGTCGTATGTTCGGCGACCGCGTCGCAGGCAAGAGTCG
CAAGTATCTCGCCAGCCAGACGTTCACGGCCAGCTACCCGTCGTTGCCCAAGC
ACCAGCGGTTCGCATCACTCCTGATGTGGTTCCTCATCTTCGGGTGCAAGTTGA
CGGAGAGTTACTTCTTCCTGACGTTGTCCTTCCGCGACCCTATTCGCGTCATGG
TCGGCATGAAGATCCAGAACTGCGAGGACAAGATTTTCGGCAGCGGCCTTTGC
AGGAATCACGCAGCATTCACCCTCACGATCATGTACATCATGGACCTCGTCTTG
TTCTTCCTCGACACCTTCCTTTGGTATGTCATCTGGAACTCGGTTTTCAGTATCG
CACGCTCTTTCGTACTCGGCCTTTCGATCTGGACACCATGGAGGGACATCTTCC
AGCGTCTGCCGAAGCGTATCTACGCGAAGCTTCTAGCGACCGGCGACATGGAG
GTCAAGTACAAGCCCAAGGTCTTGGTTTCGCAAATCTGGAACGCCATCATCATC
TCCATGTACCGCGAGCACTTGCTCTCTATCGAGCACGTTCAAAAGCTCCTGTAC
CATCAAGTGGACACTGGCGAAGCCGGCAAGCGGAGTCTTCGCGCGCCTCCGTT
CTTCGTCGCGCAGGGCAGCAGCGGTGGCTCGGGCGAGTTCTTCCCGCCTGGT
AGCGAGGCTGAGCGTCGTATCTCTTTCTTCGCGCAGTCTCTATCTACGGAGATT
CCTCAGCCCATCCCGGTTGACGCCATGCCGACGTTCACAGTGCTTACGCCTCA
CTACAGCGAGAAGATCCTTCTTTCGCTCCGTGAGATTATCCGCGAGGAGGACCA
GAACACCCGCGTGACATTGCTTGAGTATCTCAAGCAGCTTCACCCGGTCGAGTG
GGAGAACTTCGTCAAGGACACCAAGATTTTGGCCGAGGAGTCCGCTATGTTCAA
CGGTCCAAGTCCTTTCGGCAACGATGAGAAGGGTCAGTCCAAGATGGACGATC
TTCCTTTCTACTGCATCGGTTTCAAGAGCGCCGCGCCCGAGTACACCCTCCGCA
CCCGTATCTGGGCGTCCTTGCGCGCGCAGACCCTCTACCGCACGGTCTCCGGC
ATGATGAACTATGCGAAGGCGATTAAGCTGCTCTACCGCGTCGAGAACCCCGA
GGTCGTGCAGCAGTTCGGCGGTAACACGGACAAGCTCGAGCGCGAGTTGGAG
CGGATGGCCCGGCGGAAGTTCAAGTTCCTGGTGTCCATGCAGCGCTACTCGAA
GTTCAACAAGGAGGAGCACGAGAACGCCGAGTTCTTGCTCCGCGCGTACCCGG
ACCTGCAGATCGCGTACCTGGAGGAAGAGCCTCCTCGCAAGGAGGGTGGCGAT
CCACGCATCTTCTCTGCCCTCGTCGACGGCCACAGCGACATCATCCCGGAGAC
CGGCAAGCGGCGCCCCAAGTTCCGCATCGAGCTGCCCGGCAACCCCATTCTCG
GTGACGGCAAGTCGGACAACCAGAACCACGCCATCGTCTTCTACCGCGGCGAG
TACCTCCAGCTTATCGACGCCAACCAGGACAACTACCTCGAGGAGTGCTTGAAG
ATCCGTAACGTACTCGCCGAGTTCGAGGAGTACGACGTCTCTAGCCAGAGTCC
GTACGCGCAGTGGAGTGTCAAGGAGTTCAAGCGCTCCCCGGTCGCCATCGTCG
GTGCACGCGAGTATATCTTCTCGGAGCACATCGGTATTCTCGGTGATTTGGCGG
CTGGCAAGGAACAGACGTTCGGTACGCTCACGGCACGCAACAACGCCTTCCTT
GGCGGCAAGCTGCACTACGGTCACCCGGATTTCCTCAACGCCCTCTACATGAA
CACGCGCGGTGGTGTCTCCAAGGCGCAGAAGGGTCTCCATCTCAACGAGGATA
TTTACGCCGGTATGAACGCGGTCGGTCGCGGTGGACGCATCAAGCATAGCGAA
TACTACCAGTGCGGCAAGGGTCGTGACCTCGGTTTTGGCACCATCTTGAACTTC
CAGACCAAGATCGGTACGGGTATGGGCGAGCAGATCCTCTCGCGCGAGTACTA
CTACCTCGGAACCCAATTGCCCATCGATCGCTTCCTCACGTTCTACTACGCGCA
CCCAGGTTTCCAGATCAACAACATGCTGGTTATCCTATCCGTGCAGGTCTTCAT
CGTTACCATGGTCTTCCTCGGTACCTTGAAGTCTTCGGTCACGATCTGCAAGTA
CACGTCCAGCGGTCAGTACATCGGTGGTCAATCCGGTTGCTACAACCTCGTCC
CGGTCTTCCAGTGGATCGAGCGCTGCATCATCAGCATCTTCTTGGTGTTCATGA
TCGCTTTCATGCCGCTCTTCCTGCAAGAACTCGTCGAGCGCGGTACCTGGAGT
GCCATCTGGCGTCTGCTCAAGCAGTTTATGTCGCTGTCGCCTGTCTTCGAGGTG
TTCTCCACCCAGATTCAGACACACTCCGTGTTGAGCAACTTGACGTTCGGTGGT
GCGCGTTACATCGCTACCGGTCGTGGGTTCGCCACCAGTCGTATCAGCTTCAG
CATCTTGTTCTCGCGTTTCGCAGGCCCGAGTATCTACCTCGGCATGCGCACGCT
CATTATGCTGCTCTACGTGACGTTGACGATCTGGACGCCATGGGTCATTTACTT
CTGGGTTTCCATTCTCTCGCTCTGCATCGCGCCGTTCTTGTTCAATCCGCATCAA
TTCGTCTTCTCGGATTTCCTCATCGACTACAGGGAATACCTCCGGTGGATGTCG
CGTGGTAACTCGCGCTCGCACAACAACTCCTGGATTGGGTACTGCCGGTTGTC
CCGCACGATGATCACTGGGTACAAGAAGAAGAAGCTGGGCCACCCGTCGGAGA
AGCTTTCCGGCGACGTTCCTCGTGCAGGCTGGCGCGCCGTCTTATTCTCGGAG
ATCATCTTCCCGGCATGCATGGCCATCCTCTTCATCATCGCGTACATGTTCGTCA
AGTCGTTCCCTCTCGACGGCAAGCAGCCTCCCTCCGGCCTCGTTCGCATCGCC
GTCGTGTCTATCGGCCCCATCGTGTGGAACGCCGCCATCCTGTTGACGCTCTTC
CTTGTGTCGTTGTTCCTCGGCCCCATGCTCGACCCGGTCTTCCCCCTCTTCGGT
TCCGTTATGGCCTTCATCGCGCATTTCCTCGGCACAATCGGAATGATTGGGTTC
TTCGAGTTCCTGTGGTTCCTCGAGTCCTGGGAGGCGTCGCATGCCGTGCTGGG
TCTCATCGCCGTCATCTCCATCCAGCGCGCCATTCACAAAATTCTTATCGCCGTT
TTCCTCAGTCGCGAGTTCAAGCACGACGAGACGAACAGGGCTTGGTGGACTGG
TCGCTGGTATGGCCGTGGCCTCGGCACGCACGCCATGTCGCAGCCGGCGCGT
GAGTTCGTCGTCAAGATCATCGAGTTGTCGCTCTGGAGCTCGGATCTCATACTC
GGCCACATCCTGCTGTTCATGCTTACTCCGGCTGTCCTCATCCCGTACTTCGAC
CGTCTGCACGCCATGATGCTCTTCTGGCTGCGCCCCTCAAAGCAAATCCGCGC
GCCTCTGTACTCAATCAAGCAGAAGAGGCAAAGACGCTGGATTATCATGAAGTA
CGGTACTGTATACGTTACCGTCATCGCGATCTTCGTCGCGCTCATCGCGCTTCC
CCTCGTCTTCCGACACACTCTAAAGGTCGAGTGCTCCCTTTGCGACAGCTTGTA A
polypeptide sequence 1,3-.beta.-D-glucan synthase II of S. commune
strain Lu15531 amino acid S. commune SEQ ID NO: 8
MRNMFDFTMQLLDSRASRMTPNQALLTLHADYIGGQHANYRKVVYFAAQLDLDDAV
GQTQNPGLNRLKSTRGSGKRPRHEKSLNTALERWRQAMNNMSQYDRLRQIALYLL
CWGEAAQVRFMPECLCFIFKCADDYYRSPECQNRMEPVPEGLYLRTVVKPLYRFV
RDQGYEVVEGKFVRRERDHDQIIGYDDVNQLFWYPEGIARIVLSDKSRLVDLPPAQ
RFMKFDRIEWNRVFFKTFYETRSFTHLLVDFNRIWVVHIALYFFYTAYNSPTIYAING
NTPTSLAWSATALGGAVATGIMILATIAEFSHIPTTWNNTSHLTRRLAFLLVTLGLTCG
PTFYVAIAESNGSGGSLALILGIVQFFISVVATALFTIMPSGRMFGDRVAGKSRKYLA
SQTFTASYPSLPKHQRFASLLMWFLIFGCKLTESYFFLTLSFRDPIRVMVGMKIQNC
EDKIFGSGLCRNHAAFTLTIMYIMDLVLFFLDTFLVVYVIWNSVFSIARSFVLGLSIWTP
WRDIFQRLPKRIYAKLLATGDMEVKYKPKVLVSQIWNAIIISMYREHLLSIEHVQKLLY
HQVDTGEAGKRSLRAPPFFVAQGSSGGSGEFFPPGSEAERRISFFAQSLSTEIPQPI
PVDAMPTFTVLTPHYSEKILLSLREIIREEDQNTRVTLLEYLKQLHPVEWENFVKDTKI
LAEESAMFNGPSPFGNDEKGQSKMDDLPFYCIGFKSAAPEYTLRTRIWASLRAQTL
YRTVSGMMNYAKAIKLLYRVENPEVVQQFGGNTDKLERELERMARRKFKFLVSMQ
RYSKFNKEEHENAEFLLRAYPDLQIAYLEEEPPRKEGGDPRIFSALVDGHSDIIPETG
KRRPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYLQLIDANQDNYLEECLKIRNVLA
EFEEYDVSSQSPYAQWSVKEFKRSPVAIVGAREYIFSEHIGILGDLAAGKEQTFGTL
TARNNAFLGGKLHYGHPDFLNALYMNTRGGVSKAQKGLHLNEDIYAGMNAVGRGG
RIKHSEYYQCGKGRDLGFGTILNFQTKIGTGMGEQILSREYYYLGTQLPIDRFLTFYY
AHPGFQINNMLVILSVQVFIVTMVFLGTLKSSVTICKYTSSGQYIGGQSGCYNLVPVF
QWIERCIISIFLVFMIAFMPLFLQELVERGTWSAIWRLLKQFMSLSPVFEVFSTQIQTH
SVLSNLTFGGARYIATGRGFATSRISFSILFSRFAGPSIYLGMRTLIMLLYVTLTIWTP
VVVIYFVVVSILSLCIAPFLFNPHQFVFSDFLIDYREYLRWMSRGNSRSHNNSWIGYCR
LSRTMITGYKKKKLGHPSEKLSGDVPRAGWRAVLFSEIIFPACMAILFIIAYMFVKSFP
LDGKQPPSGLVRIAWSIGPIVWNAAILLTLFLVSLFLGPMLDPVFPLFGSVMAFIAHF
LGTIGMIGFFEFLWFLESWEASHAVLGLIAVISIQRAIHKILIAVFLSREFKHDETNRAW
WTGRVVYGRGLGTHAMSQPAREFVVKIIELSLWSSDLILGHILLFMLTPAVLIPYFDRL
HAMMLFWLRPSKQIRAPLYSIKQKRQRRWIIMKYGTVYVTVIAIFVALIALPLVFRHTL
KVECSLCDSL Gene sequence 1,3-.beta.-D-glucan synthase I of S.
commune strain Lu15634 DNA S. commune SEQ ID NO: 9
CCCGTCCCTCAAGGCCGTTCTTTCGCTGGCGACCGACCCGGTGTTCGCGAGAA
CCTGTTGTTTCTGACGATCATCAACCCTTTCTTCTCGTCGCTCTTTAGCTCTCCC
TAGACCGTCTTTTACTCTACTCTTCGACGCACGCCATGTCCGGTCCAGGATATG
GCAGGAATCCATTCGACAATCCCCCGCCCAACAGAGGTCCCTATGGCCAGCAG
CCAGGTTTCCCGGGGCCCGGCCCTCGGCCTTACGACTCGGACGCGGACATGA
GCCAGACCTATGGCAGCACAACCAGGCTCGCCGGCAGTGCCGGTTACAGCGA
CAGAAACGgtgcgaacgtcgctaccgtacttcctcgatcgtcgactcacatatcacgcagGC
AGCTTCGACGGCGACCGCTCCTACGCGCCCTCAATTGACTCGCGCGCCAGCGTGCCCAGC
ATATCGCCCTTCGCAGACCCGGGTATCGGCTCTAATGAGCCGTATCCCGCTTG
GTCGGTCGAACGCCAGATCCCCATGTCCACGGAGGAGATTGAGGATATCTTCC
TCGACCTCACCCAAAAGTTTGGCTTCCAGCGCGACTCCATGCGGAATACGgtgcgt
gaataagcagcccactcgaccgcgggaacagctcaattgacctgtcacccagTTCGACTTCA
TGATGCACCTCCTTGATTCCCGTGCCTCGCGCATGACGCCCAACCAAGCTCTGCTCACGCT
TCACGCCGACTACATTGGTGGCCAGCACGCCAACTATAGGAAGTGGTATTTCGC
CGCTCAGCTCAACCTCGATGACGCGGTCGGGCAAACCAATAACCCCGGTATCC
AGCGCTTGAAGACCATCAAGGGCGCTACGAAGACCAAGTCGCTCGACAGCGCA
CTCAACCGCTGGCGCAATGCGATGAACAACATGAGCCAGTACGATCGCCTCCG
GCAAATTGCGCTCTATCTCCTCTGCTGGGGAGAAGCAGGCAACATCCGTCTGG
CGCCCGAGTGCTTGTGCTTCATCTTCAAGTGCGCGGACGACTACTACAGAAGTC
CCGAGTGTCAGAACCGGATGGACCCCGTGCCGGAAGGGCTGTACCTCCAGAC
GGTCATCAAGCCGCTCTATCGCTTCCTACGTGATCAGGCGTACGAAGTCGTTGA
TGGGAAGCAAGTGAAGCGCGAGAAGGACCACGACCAGATTATCGGTTATGACG
ACGTCAACCAGTTATTCTGGTATCCGGAAGGTTTGGCTAAGATCGTCATGTCGG
ACAACgtgcgtatgatcttatcggttacaattcgtccgctcacatctttccagACACGACT
TGTAGATGTACCTCCGGCGCAGCGGTTCATGAAGTTCGCCAAGATCGAGTGGAACCGCGTCTTC
TTCAAGACGTACTTTGAGAAGCGCTCTACTGCCCATCTCCTGGTCAACTTCAAC
CGTATATGGATCCTCCACGTCTCGATGTACTTCTTCTACACGGCATTCAACTCTC
CACGAGTCTACGCGCCGCACGGCAAACTCGACCCCTCCCCTGAGATGACCTGG
TCCGCGACTGCCCTTGGAGGCGCTGTGTCCACCATGATCATGATCCTTGCCACT
ATCGCGGAGTACACCTACATCCCCACGACATGGAACAATGCGTCGCACCTCAC
CACGCGGCTCATTTTCCTCCTGGTCATCCTCGCGCTCACTGCTGGACCAACATT
CTATATCGCCATGATAGACGGACGCACGGACATCGGCCAAGTACCACTCATCGT
GGCCATAGTGCAGTTCTTCATCTCCGTCGTCGCCACCCTCGCTTTCGCTACCAT
CCCTTCTGGTCGCATGTTCGGCGACCGTGTGGCTGGCAAGTCAAGAAAGCACA
TGGCATCGCAGACGTTCACAGCGTCGTACCCGTCCATGAAGCGGTCATCTCGC
GTAGCGAGTATCATGCTGTGGCTTTTGGTCTTTGGCTGCAAATACGTCGAGTCT
TACTTCTTCTTGACGTCCTCCTTCTCCAGCCCGATCGCGGTCATGGCGCGTACG
AAGGTACAGGGCTGCAACGACCGTATCTTCGGCAGCCAGCTGTGCACGAATCA
GGTCCCGTTCGCGCTGGCAATCATGTACGTGATGGACCTGGTACTGTTCTTCCT
GGACACGTACCTGTGGTACATCATCTGGCTGGTGATCTTCTCGATGGTGCGCG
CGTTCAAGCTTGGTATCTCGATCTGGACGCCCTGGAGCGAGATCTTCACCCGCA
TGCCGAAGCGTATCTACGCGAAGCTGCTGGCGACGGCCGAGATGGAGGTCAA
GTATAAGCCCAAGgtatgctgaatgcaatctggtcaggtgaattcaccctcatattgttgtg
cagGTGCTCGTCTCGCAAATCTGGAACGCGGTCATCATCTCCATGTACCGGGAGCATCTCTTGT
CCATCGAGCACGTCCAGCGCCTGCTATACCACCAGGTTGATGGTCCAGACGGT
CGCCGCACCCTCAGGGCACCGCCGTTCTTCACCAGCCAGCGAACTGCGAAGCC
AGGCCTGTTCTTCCCTCCTGGTGGCGAGGCTGAGCGCCGTATCTCGTTCTTTGC
CTCATCGCTGACGACCGCGCTCCCTGAGCCTCTGCCGATCGACGCCATGCCCA
CCTTCACCGTGCTCGTTCCCCATTACTCGGAGAAGATTCTGCTCAGTCTGCGCG
AGATTATTCGCGAGGAGGACCAGAACACCCGCGTCACCTTGCTGGAGTACCTC
AAGCAGCTCCACCCTGTCGAATGGGACAACTTCGTCAAGGACACCAAGATCTTG
GCGGAAGAGTCGGGCGACGTCCAGGACGAGAAGCGCGCGCGCACGGACGACT
TGCCGTTCTACTGCATCGGGTTCAAGACCTCGTCACCAGAGTACACCCTGCGTA
CGCGTATCTGGGCTTCACTGCGCGCACAGACGCTGTACCGCACGGTCTCCGGT
ATGATGAACTACTCCAAGGCGATCAAGCTCCTCTATCGCGTCGAGAACCCGGAT
GTCGTTCATGCCTTCGGTGGGAACACGGAACGTCTTGAACGCGAGCTTGAGCG
CATGTCTCGCCGCAAGTTCAAGTTCGTCATCTCGATGCAGCGGTACTCTAAGTT
CAACAAGGAGGAGCAAGAGAACGCCGAATTCCTTCTGCGCGCGTACCCGGATT
TGCAGATCGCGTACCTCGATGAAGAGCCCGGTCCCAGCAAGAGCGACGAGGTT
CGGTTGTTTTCGACACTCATCGATGGACACTCCGAGGTGGATGAGAAGACCGG
CCGCCGCAAGCCCAAGTTCCGCATTGAGCTGCCCGGTAACCCCATCCTCGGTG
ACGGGAAGTCGGATAACCAGAACCACGCCATTGTCTTCTACCGCGGCGAGTAC
ATCCAGGTCATCGACGCTAACCAGGACAATTACCTGGAAGAGTGTCTCAAGATC
CGTAACGTCCTGGGCGAGTTTGAGGAATACTCCGTGTCGAGCCAGAGCCCGTA
CGCACAGTGGGGCCACAAGGAGTTCAACAAGTGCCCCGTCGCTATCCTGGGTT
CTCGCGAGTACATCTTCTCGGAGAACATCGGTATCCTCGGTGACATCGCCGCC
GGCAAGGAACAGACGTTCGGTACCATTACGGCGCGTGCGCTTGCGTGGATCGG
CGGCAAGCTGCATTACGGTCACCCGGATTTCCTCAATGCGACGTTCATGACGAC
GCGTGGTGGCGTGTCAAAAGCGCAGAAGGGCTTGCATCTCAACGAGGATATCT
TCGCTGGTATGACCGCCGTGTCCCGCGGAGGGCGCATCAAGCACATGGAGTAC
TACCAGTGCGGCAAAGGTCGTGATCTCGGTTTCGGCACGATCTTGAACTTCCAG
ACGAAGATCGGTACTGGTATGGGCGAGCAGCTCCTCTCGCGCGAGTACTACTA
CCTGGGCACGCAATTGCCTATCGACCGGTTCTTGACGTTCTACTACGCGCACGC
TGGTTTCCACGTCAACAACATCCTGGTCATCTACTCCATCCAGGTCTTCATGGTC
ACCTgtaagtgcaggcgctcatgaccgccgagaacgtagtctgacggatgtgcagTGCTGTA
CCTGGGCACATTGAACAAGCAGCTGTTCATCTGCAAGGTCAACTCCAATGGCCAGGTTCT
TAGTGGACAAGCTGGGTGCTACAACCTCATCCCGGTCTTCGAGTGGATTCGCC
GGAGTATCATCTCCATCTTCTTGGTGTTCTTCATCGCCTTCTTGCCTCTATTCTT
GCAAGgtatgttcactttccatgtgtcatccgttagccgctcaccatacgacagAGCTGTGCG
AGCGCGGAACGGGAAAGGCGTTGCTGCGTCTCGGGAAGCACTTCTTGTCACTGTCGCCCA
TTTTCGAAGTGTTCTCCACCCAGATTTACTCGCAGGCGCTCTTGAACAACATGA
GCTTCGGTGGTGCGCGCTACATCGCCACAGGTCGTGGTTTCGCGACTAGTCGC
ATACCCTTCAACATCCTCTACTCGCGTTTCGCGCCGCCAAGCATCTACATGGGC
ATGCGTAACCTGCTGCTCCTGCTGTACGCGACGATGGCCATTTGGATCCCGCA
CCTGATCTACTTCTGGTTCTCCGTCCTCTCCCTCTGCATCGCGCCATTCATGTTC
AATCCGCATCAATTCTCGTACGCCGACTTCATCATCGACTACCGGGAGTTCTTG
CGCTGGATGTCGCGCGGTAACTCGCGAACGAAGGCGAGCAGCTGGTACGGAT
ACTGCCGTCTGTCGCGTACCGCGATTACTGGGTACAAGAAGAAGAAGCTGGGA
CACCCGTCGGAGAAGCTGTCGGGCGACGTACCGCGTGCGCCGTGGAGGAACG
TTATCTTCTCGGAGATCCTGTGGCCCATCGGCGCGTGCATCATCTTCATCGTCG
CGTACATGTTCGTCAAGTCGTTCCCCGACGAGCAGGGCAACGCGCCGCCGAGC
CCGCTGGTCCGGATTCTGCTCATCGCGGTTGGCCCTACTGTGTGGAACGCGGC
GGTGCTCATAACGCTGTTCTTCCTGTCGCTCTTCCTGGGCCCGATGATGGATGG
CTGGGTCAAGTTCGGCTCGGTCATGGCGGCCCTTGCGCATGGCCTGGCGCTTA
TAGGCATGCTCACGTTCTTTGAGTTCTTCgtacgtccttcgcgttgtgtcgtcaagtgctctg
ctaacgccgtcttcagTGGTTCCTTGAGCTCTGGGATGCCTCGCACGCCGTGCTCGGCGTCAT
CGCTATCATTGCCGTTCAGCGCGGGATCCAGAAGATCCTCATTGCCGTCTTCCT
GACGCGTGAGTACAAGCACGACGAGACGAACCGCGCGTGGTGGACAGGTAAAT
GGTATGGACGCGGGCTGGGTACCTCGGCCATGTCCCAGCCGGCGCGCGAGTT
CATCGTGAAGATCGTGGAGATGTCGTTGTGGACGTCGGACTTCCTGCTTGCGC
ACCTGTTGCTCATCATCTTGACGGTGCCGCTACTGCTGCCGTTCTTCAACTCAAT
TCATTCGACGATGCTTTgtgagtggtttgtagtcgttggtcatggatgatttctgactcgcg
tgcagTCTGGTTGCGCCCTTCGAAGCAGATTAGGCAACCTCTGTTCTCCACCAAGCAGAAGCGG
CAACGGCGATGGATTgtgagttcctttgattgctctgggtaccgaccttcgctcacctttctt
agGTCATGAAGTATACCGTGGTATATCTCGTGGTGGTGGCTTTCCTCGTCGCGCTCATCGCTCT
GCgtacgttttccctcgcgctcaccctgtattttcactaacgtttcctccagCCGCCCTCTTC
CGCGAGAGCATCCACTTCAACTGCGAGATCTGCCAGAGTATATAGTCATATAACGACGTCTATC
GTATCGCCGGACGAGAGCCCCGTCGCCTACACACTGACATGGAATCGCTGTGT
ATACAATCGATCTTCTGACCGCGTCGGGGGCGTTGCCGTCTTTCTACTATCAAT
TTGCTTGTGTATCAACATTTCTTCTCTCCAAGCCTACATTGACATAGAGTAATAG
CCCATGTTCATACAACAATCGCATAGCATTGCATATACCAT translation of SEQ ID NO:
13 amino acid S. commune SEQ ID NO: 10
MSGPGYGRNPFDNPPPNRGPYGQQPGFPGPGPRPYDSDADMSQTYGSTTRLAG
SAGYSDRNGSFDGDRSYAPSIDSRASVPSISPFADPGIGSNEPYPAWSVERQIPMS
TEEIEDIFLDLTQKFGFQRDSMRNTFDFMMHLLDSRASRMTPNQALLTLHADYIGGQ
HANYRKWYFAAQLNLDDAVGQTNNPGIQRLKTIKGATKTKSLDSALNRWRNAMNN
MSQYDRLRQIALYLLCWGEAGNIRLAPECLCFIFKCADDYYRSPECQNRMDPVPEG
LYLQTVIKPLYRFLRDQAYEVVDGKQVKREKDHDQIIGYDDVNQLFWYPEGLAKIVM
SDNTRLVDVPPAQRFMKFAKIEWNRVFFKTYFEKRSTAHLLVNFNRIWILHVSMYFF
YTAFNSPRVYAPHGKLDPSPEMTWSATALGGAVSTMIMILATIAEYTYIPTTWNNAS
HLTTRLIFLLVILALTAGPTFYIAMIDGRTDIGQVPLIVAIVQFFISVVATLAFATIPSGRM
FGDRVAGKSRKHMASQTFTASYPSMKRSSRVASIMLWLLVFGCKYVESYFFLTSSF
SSPIAVMARTKVQGCNDRIFGSQLCTNQVPFALAIMYVMDLVLFFLDTYLWYIIWLVI
FSMVRAFKLGISIWTPWSEIFTRMPKRIYAKLLATAEMEVKYKPKVLVSQIWNAVIISM
YREHLLSIEHVQRLLYHQVDGPDGRRTLRAPPFFTSQRTAKPGLFFPPGGEAERRIS
FFASSLTTALPEPLPIDAMPTFTVLVPHYSEKILLSLREIIREEDQNTRVTLLEYLKQLH
PVEWDNFVKDTKILAEESGDVQDEKRARTDDLPFYCIGFKTSSPEYTLRTRIWASLR
AQTLYRTVSGMMNYSKAIKLLYRVENPDVVHAFGGNTERLERELERMSRRKFKFVI
SMQRYSKFNKEEQENAEFLLRAYPDLQIAYLDEEPGPSKSDEVRLFSTLIDGHSEVD
EKTGRRKPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYIQVIDANQDNYLEECLKIR
NVLGEFEEYSVSSQSPYAQWGHKEFNKCPVAILGSREYIFSENIGILGDIAAGKEQTF
GTITARALAWIGGKLHYGHPDFLNATFMTTRGGVSKAQKGLHLNEDIFAGMTAVSR
GGRIKHMEYYQCGKGRDLGFGTILNFQTKIGTGMGEQLLSREYYYLGTQLPIDRFLT
FYYAHAGFHVNNLVIYSIQVFMVTLLYLGTLNKQLFICKVNSNGQVLSGQAGCYNLI
PVFEWIRRSIISIFLVFFIAFLPLFLQELCERGTGKALLRLGKHFLSLSPIFEVFSTQIYS
QALLNNMSFGGARYIATGRGFATSRIPFNILYSRFAPPSIYMGMRNLLLLLYATMAIW
IPHLIYFWFSVLSLCIAPFMFNPHQFSYADFIIDYREFLRWMSRGNSRTKASSWYGY
CRLSRTAITGYKKKKLGHPSEKLSGDVPRAPWRNVIFSEILWPIGACIIFIVAYMFVKS
FPDEQGNAPPSPLVRILLIAVGPTVWNAAVLITLFFLSLFLGPMMDGVVVKFGSVMAA
LAHGLALIGMLTFFEFFWFLELWDASHAVLGVIAIIAVQRGIQKILIAVFLTRKVVYGRG
LGTSAMSQPAREFIVKIVEMSLWTSDFLLAHLLLIILTVPLLLPFFNSIHSTMLFWLRPS
KQIRQPLFSTKQKRQRRWIVMKYTVVYLVVVAFLVALIALPALFRESIHFNCEICQSI Gene
sequence 1,3-.beta.-D-glucan synthase II of S. commune strain
Lu15634 DNA S. commune SEQ ID NO: 11
CTGTCCAAGGAGGAGATCGAGGACATCTTCCTCGATTTGACGCAGAAGTTTGGC
TTTCAGCGGGATTCCATGCGGAATATGgtacgtggcgtgtgcccatgtgcggcgttctgagg
cctaacgttttccgccagTTCGACTTCACCATGCAGCTGCTTGACAGCCGAGCGTCTCGTATG
ACCCCCAACCAGGCGCTCCTCACCCTCCACGCCGACTACATTGGTGGCCAGCA
TGCGAACTACCGGAAGTGGTACTTCGCGGCGCAGCTCGACCTTGACGACGCCG
TGGGACAAACTCAGAATCCGGGTCTCAACCGCCTCAAGTCCACTCGCGGATCG
GGCAAGCGACCACGCCATGAAAAGTCGCTGAACACGGCATTGGAGCGCTGGC
GGCAAGCCATGAACAACATGTCGCAGTATGACCGCTTACGCCAGATCGCGCTC
TACCTGCTCTGCTGGGGCGAAGCGGCGCAAGTGCGATTCATGCCCGAGTGCTT
GTGCTTCATCTTCAAGTGCGCCGACGACTACTATCGTTCGCCGGAGTGCCAGAA
CAGGATGGAGCCGGTACCGGAGGGTCTCTACCTGAGGACGGTCGTAAAGCCG
CTCTACAGATTTGTCCGGGATCAAGGCTATGAGGTGGTGGAGGGAAAATTCGTA
CGGCGGGAACGGGATCACGACCAAATCATTGGTTACGATGACGTGAATCAGCT
GTTCTGGTACCCGGAGGGAATTGCCCGTATCGTCCTGTCGGACAAGgtaagcacctc
tgtgcatcttctgtgacatacagggctaattgtcgagcagAGTCGTCTAGTCGACCTCCCCCC
AGCACAGCGCTTCATGAAGTTCGACCGTATCGAGTGGAATCGCGTCTTCTTCAAGACG
TTTTACGAGACTCGATCCTTCACGCATCTTTTGGTCGACTTCAACCGTATCTGGG
TCGTGCACATCGCTCTCTACTTCTTCTACACTGCATACAACTCCCCCACGATCTA
CGCCATCAACGGCAACACACCGACGTCTCTGGCTTGGAGCGCGACTGCGCTCG
GCGGTGCGGTAGCGACAGGTATCATGATCCTCGCCACGATCGCCGAGTTCTCG
CACATCCCCACGACATGGAACAACACCTCGCATCTGACTCGCCGCCTCGCCTTC
CTCCTCGTCACGCTCGGCCTCACATGTGGTCCGACGTTCTACGTCGCGATTGCA
GAGAGCAACGGGAGCGGCGGCTCTTTGGCCTTGATTCTCGGTATCGTCCAGTT
CTTCATCTCCGTCGTGGCAACTGCGCTCTTCACTATCATGCCTTCTGGTCGTAT
GTTCGGCGACCGTGTCGCAGGCAAGAGTCGCAAGTATCTCGCCAGCCAGACGT
TCACGGCCAGCTACCCGTCGTTGCCCAAGCACCAGCGGTTCGCCTCACTCCTG
ATGTGGTTCCTCATCTTCGGGTGCAAGTTGACGGAGAGTTACTTCTTTCTGACG
CTGTCCTTCCGCGACCCTATCCGCGTCATGGTCGGCATGAAGATCCAGAACTG
CGAGGACAAGATTTTCGGCAGCGGCCTTTGCAGGAATCACGCAGCATTCACCC
TCACGATCATGTACATCATGGACCTCGTCTTGTTCTTCCTCGACACCTTCCTTTG
GTATGTCATCTGGAACTCGGTTTTCAGTATCGCACGCTCTTTCGTACTCGGCCTT
TCGATCTGGACACCGTGGAGAGACATCTTCCAGCGTCTGCCGAAGCGGATCTA
CGCGAAGCTTCTGGCGACTGGCGACATGGAGGTCAAGTACAAGCCCAAGgtatgc
gttgagctcgccgtaaatccacttaaggctaacacgttcgcagGTCTTGGTCTCGCAAATCT
GGAACGCCATCATCATCTCCATGTACCGCGAGCACTTGCTCTCTATTGAGCACGTCCAG
AAGCTCCTGTACCACCAAGTGGACACTGGCGAAGCCGGCAAGCGGAGTCTTCG
CGCGCCTCCGTTCTTCGTCGCGCAGGGCAGCAGCGGTGGCTCGGGCGAGTTC
TTCCCGCCTGGCAGCGAGGCCGAGCGTCGTATCTCTTTCTTCGCGCAGTCGCT
TTCTACGGAGATTCCTCAGCCCATCCCGGTCGACGCCATGCCGACGTTCACGG
TGCTTACGCCTCACTACAGCGAGAAGgtacatgctccccttgtagccatatgacatcagctga
ctgtcgtgcacagATCCTTCTCTCTCTCCGTGAAATTATCCGCGAGGAGGACCAGAACACT
CGCGTTACGTTGCTCGAGTACCTGAAGCAGCTGCATCCGGTCGAGTGGGAGAA
TTTCGTCAAGGACACTAAAATTTTGGCCGAGGAGTCCGCTATGTTTAACGGTCC
GAGTCCTTTCGGCAACGACGAGAAGGGTCAGTCCAAGATGGACGATCTACCGT
TCTACTGCATCGGTTTCAAGAGCGCCGCGCCCGAGTACACCCTCCGCACCCGT
ATCTGGGCGTCCCTGCGCGCGCAGACGCTGTACCGCACGGTCTCCGGCATGAT
GAACTATGCGAAGGCGATCAAGCTGCTCTACCGCGTTGAGAACCCGGAGGTCG
TACAACAGTTCGGCGGCAACACGGACAAGCTCGAGCGCGAGTTGGAGCGGATG
GCGCGACGGAAGTTCAAGTTCCTCGTGTCCATGCAGCGCTACTCGAAGTTCAAC
AAGGAGGAGCACGAGAACGCCGAGTTCTTGCTCCGCGCGTACCCGGACTTGCA
GATCGCGTACCTCGAGGAAGAGCCCCCTCGCAAGGAGGGCGGCGATCCACGC
ATCTTCTCTGCCCTCGTCGACGGCCACAGCGACATCATCCCGGAGACCGGCAA
GCGGCGCCCCAAGTTCCGTATCGAGCTGCCCGGTAACCCCATTCTCGGTGACG
GTAAATCCGACAATCAGAACCACGCTATCGTCTTCTACCGCGGCGAGTACCTCC
AGCTTATCGACGCCAACCAGGACAACTACCTCGAGGAGTGCTTGAAGATCCGTA
ACGTGCTCGCCGAGTTTGAGGAGTACGACGTCTCCAGCCAGAGCCCGTACGCG
CAGTGGAGTGTCAAGGAGTTCAAGCGCTCTCCGGTCGCCATCGTCGGTGCACG
CGAGTACATCTTCTCAGAGCACATCGGTATCCTCGGTGATCTGGCGGCTGGCAA
GGAACAGACGTTCGGTACGCTCACGGCACGCAACAACGCCTTCCTTGGCGGCA
AGCTGCACTACGGTCACCCCGATTTCCTCAACGCCCTCTACATGAACACGCGCG
GTGGTGTCTCCAAGGCGCAGAAGGGTCTCCATCTCAACGAGGATATCTACGCC
GGTATGAACGCGGTCGGTCGCGGTGGACGCATTAAGCACAGCGAGTACTATCA
GTGCGGCAAGGGTCGTGACCTCGGTTTCGGCACCATCTTGAACTTCCAGACCA
AGATCGGTACGGGTATGGGCGAGCAGATCCTCTCGCGCGAGTACTACTATCTC
GGAACACAACTGCCCATCGATCGCTTCCTCACGTTCTACTACGCGCACCCGGGT
TTCCAGATCAACAACATGCTGGTCATCCTCTCCGTGCAGGTCTTCATCGTTACCA
gtacgttcaatgcatattgttagcctgacaacgtctgacgaatttccagTGGTCTTCCTCGGT
ACCTTGAAGTCTTCGGTCACGATCTGCAAGTACACGTCCAGCGGTCAGTACATCGGTGGTCA
ATCCGGTTGCTACAACCTCGTCCCGGTCTTCCAGTGGATCGAGCGCTGCATCAT
CAGCATCTTCTTGGTGTTCATGATCGCTTTCATGCCGCTCTTCCTGCAAGgtaaga
gcttgtcaacctgctcaaggggcttgcgctgatcatcatctcagAACTCGTCGAGCGCGGTACC
TGGAGTGCCATCTGGCGTCTGCTCAAGCAGTTTATGTCGCTGTCGCCTGTCTTCGAGG
TGTTCTCCACCCAGATTCAGACGCACTCCGTGTTGAGCAACTTGACGTTCGGTG
GTGCGCGTTACATCGCTACCGGTCGTGGGTTCGCCACCAGTCGTATCAGCTTC
AGCATCTTGTTCTCGCGTTTCGCAGGCCCGAGTATCTACCTCGGCATGCGCACG
CTCATTATGCTGCTCTACGTGACGTTGACGATCTGGACGCCATGGGTCATTTAC
TTCTGGGTTTCCATTCTCTCGCTCTGCATCGCGCCGTTCTTGTTCAACCCGCATC
AATTCGTATTCTCGGACTTCCTCATCGACTACAGgtacgtcggacgagcgctgttccgcgacgt
aagctgaccggttatacagGGAATACCTGCGGTGGATGTCGCGTGGCAACTCGCGCTCG
CACAACAACTCCTGGATTGGGTACTGCCGGTTGTCCCGCACGATGATCACTGG
GTACAAGAAGAAGAAGCTGGGCCACCCGTCGGAGAAGCTTTCCGGCGACGTTC
CTCGTGCAGGCTGGCGCGCCGTCTTGTTCTCGGAGATCATCTTCCCGGCGTGC
ATGGCCATCCTCTTCATCATCGCGTACATGTTCGTCAAGTCGTTCCCTCTCGAC
GGCAAGCAGCCTCCCTCCGGCCTCGTTCGCATCGCCGTCGTGTCTATCGGCCC
CATCGTGTGGAACGCCGCCATCCTGTTGACGCTCTTCCTTGTGTCGTTGTTCCT
CGGCCCCATGCTCGACCCGGTCTTCCCCCTCTTCGGTTCCGTTATGGCCTTCAT
CGCGCATTTCCTTGGCACAATCGGAATGATTGGGTTCTTCGAGTTCCTGgtatgtgc
ccatacctttcattcgacttcaactatctaacagattcatagTGGTTCCTCGAGTCCTGGGAG
GCGTCGCATGCCGTGCTGGGTCTCATCGCCGTCATCTCCATCCAGCGCGCCATTCACA
AGATCCTTATCGCCGTTTTCCTCAGTCGCGAGTTCAAGCACGACGAGACGAACA
GGGCCTGGTGGACTGGTCGCTGGTATGGCCGTGGCCTCGGCACGCACGCCAT
GTCGCAGCCGGCGCGTGAGTTCGTCGTCAAGATCATCGAGTTGTCGCTTTGGA
GCTCGGATCTCATACTCGGCCACATCCTGCTGTTCATGCTTACTCCGGCCGTCC
TCATCCCGTACTTCGACCGTTTGCACGCCATGATGCTCTgtacgtcgtgtctcattgtctgtgt
tggtcatactcttaccctctcttagTCTGGCTGCGTCCCTCGAAGCAAATCCGCGCGCCTCTG
TACTCGATCAAGCAGAAGAGGCAAAGACGCTGGATTgtcagtgttcagtgccttattctatcag
ctcttactaacgtcttcatagATCATGAAGTACGGTACTGTATACGTTACCGTCATCGCGAT
CTTCGTCGCGCTCATCGCGCTTCgtgagtttccttgctatttttcgtacctgagcgtcgctgac
ccctttcccagCCCTCGTATTCCGACACACTCTAAAGGTCGAGTGCTCCCTTTGCGACAGCTT
GTAATATCGGACTCGTATATATCTAGACTTCTCCGCACCATGTGTAGCTGACGCT
TGGGTATACTTCGCGGTGCCGAGCTAATTGTCGACGGACATTCTCCATCGTTGA
GTGCAGCGACGTCGGGTGGTTTACGACACGGACACTTTTCATTGTACCCTCTAC
GAATGCAAGAACTCTCTTACGACCAGTACCTATGTGCTAAGCCGTCGCCTGTTC
AGGATCATACATACATACGTTTCTAGATACCTTACAGTTAGGCCTATTCAGGGAG
AGTCTGCATAAAA translation of SEQ ID NO: 15 amino acid S. commune
SEQ ID NO: 12
MPRPGGTSAEGGYASSPSMETTPSDPFGTANGAPRRYYDNDSEEYGPGRRDTYA
SDSSNQGLTDPGYYDQNGAYDPYPTGDTDSDGDVYGQRYGPSAESLGTHKFGHS
DSSTPTFVDYSASSGGRDSYPAWTAERNIPLSKEEIEDIFLDLTQKFGFQRDSMRN
MFDFTMQLLDSRASRMTPNQALLTLHADYIGGQHANYRKVVYFAAQLDLDDAVGQT
QNPGLNRLKSTRGSGKRPRHEKSLNTALERWRQAMNNMSQYDRLRQIALYLLCW
GEAAQVRFMPECLCFIFKCADDYYRSPECQNRMEPVPEGLYLRTVVKPLYRFVRD
QGYEWEGKFVRRERDHDQIIGYDDVNQLFWYPEGIARIVLSDKSRLVDLPPAQRF
MKFDRIEWNRVFFKTFYETRSFTHLLVDFNRIVVWHIALYFFYTAYNSPTIYAINGNT
PTSLAWSATALGGAVATGIMILATIAEFSHIPTTWNNTSHLTRRLAFLLVTLGLTCGPT
FYVAIAESNGSGGSLALILGIVQFFISVVATALFTIMPSGRMFGDRVAGKSRKYLASQ
TFTASYPSLPKHQRFASLLMWFLIFGCKLTESYFFLTLSFRDPIRVMVGMKIQNCED
KIFGSGLCRNHAAFTLTIMYIMDLVLFFLDTFLWYVIWNSVFSIARSFVLGLSIWTPWR
DIFQRLPKRIYAKLLATGDMEVKYKPKVLVSQIWNAIIISMYREHLLSIEHVQKLLYHQ
VDTGEAGKRSLRAPPFFVAQGSSGGSGEFFPPGSEAERRISFFAQSLSTEIPQPIPV
DAMPTFTVLTPNYSEKILLSLREIIREEDQNTRVTLLEYLKQLHPVEWENFVKDTKILA
EESAMFNGPSPFGNDEKGQSKMDDLPFYCIGFKSAAPEYTLRTRIWASLRAQTLYR
TVSGMMNYAKAIKLLYRVENPEVVQQFGGNTDKLERELERMARRKFKFLVSMQRY
SKFNKEEHENAEFLLRAYPDLQIAYLEEEPPRKEGGDPRIFSALVDGHSDIIPETGKR
RPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYLQLIDANQDNYLEECLKIRNVLAEF
EEYDVSSQSPYAQWSVKEFKRSPVAIVGAREYIFSEHIGILGDLAAGKEQTFGTLTA
RNNAFLGGKLHYGHPDFLNALYMNTRGGVSKAQKGLHLNEDIYAGMNAVGRGGRI
KHSEYYQCGKGRDLGFGTILNFQTKIGTGMGEQILSREYYYLGTQLPIDRFLTFYYA
HPGFQINNMLVILSVQVFIVTMVFLGTLKSSVTICKYTSSGQYIGGQSGCYNLVPVFQ
WIERCIISIFLVFMIAFMPLFLQELVERGTWSAIWRLLKQFMSLSPVFEVFSTQIQTHS
VLSNLTFGGARYIATGRGFATSRISFSILFSRFAGPSIYLGMRTLIMLLYVTLTIWTPW
VIYFWVSILSLCIAPFLFNPHQFVFSDFLIDYREYLRWMSRGNSRSHNNSWIGYCRL
SRTMITGYKKKKLGHPSEKLSGDVPRAGWRAVLFSEIIFPACMAILFIIAYMFVKSFPL
DGKQPPSGLVRIAVVSIGPIVVVNAAILLTLFLVSLFLGPMLDPVFPLFGSVMAFIAHFL
GTIGMIGFFEFLWFLESWEASHAVLGLIAVISIQRAIHKILIAVFLSREFKHDETNRAW
WTGRVVYGRGLGTHAMSQPAREFVVKIIELSLWSSDLILGHILLFMLTPAVLIPYFDRL
HAMMLFWLRPSKQIRAPLYSIKQKRQRRWIIMKYGTVYVTVIAIFVALIALPLVFRHTL
KVECSLCDSL cDNA 1,3-13-D-glucan synthase I of S. commune strain
Lu15634 DNA S. commune SEQ ID NO: 13
ATGTCCGGTCCAGGATATGGCAGGAATCCATTCGACAATCCCCCGCCCAACAG
AGGTCCCTATGGCCAGCAGCCAGGTTTCCCGGGGCCCGGCCCTCGGCCTTAC
GACTCGGACGCGGACATGAGCCAGACCTATGGCAGCACAACCAGGCTCGCCG
GCAGTGCCGGTTACAGCGACAGAAACGGCAGCTTCGACGGCGACCGCTCCTAC
GCGCCCTCAATTGACTCGCGCGCCAGCGTGCCCAGCATATCGCCCTTCGCAGA
CCCGGGTATCGGCTCTAATGAGCCGTATCCCGCTTGGTCGGTCGAACGCCAGA
TCCCCATGTCCACGGAGGAGATTGAGGATATCTTCCTCGACCTCACCCAAAAGT
TTGGCTTCCAGCGCGACTCCATGCGGAATACGTTCGACTTCATGATGCACCTCC
TTGATTCCCGTGCCTCGCGCATGACGCCCAACCAAGCTCTGCTCACGCTTCACG
CCGACTACATTGGTGGCCAGCACGCCAACTATAGGAAGTGGTATTTCGCCGCTC
AGCTCAACCTCGATGACGCGGTCGGGCAAACCAATAACCCCGGTATCCAGCGC
TTGAAGACCATCAAGGGCGCTACGAAGACCAAGTCGCTCGACAGCGCACTCAA
CCGCTGGCGCAATGCGATGAACAACATGAGCCAGTACGATCGCCTCCGGCAAA
TTGCGCTCTATCTCCTCTGCTGGGGAGAAGCAGGCAACATCCGTCTGGCGCCC
GAGTGCTTGTGCTTCATCTTCAAGTGCGCGGACGACTACTACAGAAGTCCCGAG
TGTCAGAACCGGATGGACCCCGTGCCGGAAGGGCTGTACCTCCAGACGGTCAT
CAAGCCGCTCTATCGCTTCCTACGTGATCAGGCGTACGAAGTCGTTGATGGGAA
GCAAGTGAAGCGCGAGAAGGACCACGACCAGATTATCGGTTATGACGACGTCA
ACCAGTTATTCTGGTATCCGGAAGGTTTGGCTAAGATCGTCATGTCGGACAACA
CACGACTTGTAGATGTACCTCCGGCGCAGCGGTTCATGAAGTTCGCCAAGATC
GAGTGGAACCGCGTCTTCTTCAAGACGTACTTTGAGAAGCGCTCTACTGCCCAT
CTCCTGGTCAACTTCAACCGTATATGGATCCTCCACGTCTCGATGTACTTCTTCT
ACACGGCATTCAACTCTCCACGAGTCTACGCGCCGCACGGCAAACTCGACCCC
TCCCCTGAGATGACCTGGTCCGCGACTGCCCTTGGAGGCGCTGTGTCCACCAT
GATCATGATCCTTGCCACTATCGCGGAGTACACCTACATCCCCACGACATGGAA
CAATGCGTCGCACCTCACCACGCGGCTCATTTTCCTCCTGGTCATCCTCGCGCT
CACTGCTGGACCAACATTCTATATCGCCATGATAGACGGACGCACGGACATCGG
CCAAGTACCACTCATCGTGGCCATAGTGCAGTTCTTCATCTCCGTCGTCGCCAC
CCTCGCTTTCGCTACCATCCCTTCTGGTCGCATGTTCGGCGACCGTGTGGCTG
GCAAGTCAAGAAAGCACATGGCATCGCAGACGTTCACAGCGTCGTACCCGTCC
ATGAAGCGGTCATCTCGCGTAGCGAGTATCATGCTGTGGCTTTTGGTCTTTGGC
TGCAAATACGTCGAGTCTTACTTCTTCTTGACGTCCTCCTTCTCCAGCCCGATCG
CGGTCATGGCGCGTACGAAGGTACAGGGCTGCAACGACCGTATCTTCGGCAGC
CAGCTGTGCACGAATCAGGTCCCGTTCGCGCTGGCAATCATGTACGTGATGGA
CCTGGTACTGTTCTTCCTGGACACGTACCTGTGGTACATCATCTGGCTGGTGAT
CTTCTCGATGGTGCGCGCGTTCAAGCTTGGTATCTCGATCTGGACGCCCTGGA
GCGAGATCTTCACCCGCATGCCGAAGCGTATCTACGCGAAGCTGCTGGCGACG
GCCGAGATGGAGGTCAAGTATAAGCCCAAGGTGCTCGTCTCGCAAATCTGGAA
CGCGGTCATCATCTCCATGTACCGGGAGCATCTCTTGTCCATCGAGCACGTCCA
GCGCCTGCTATACCACCAGGTTGATGGTCCAGACGGTCGCCGCACCCTCAGGG
CACCGCCGTTCTTCACCAGCCAGCGAACTGCGAAGCCAGGCCTGTTCTTCCCT
CCTGGTGGCGAGGCTGAGCGCCGTATCTCGTTCTTTGCCTCATCGCTGACGAC
CGCGCTCCCTGAGCCTCTGCCGATCGACGCCATGCCCACCTTCACCGTGCTCG
TTCCCCATTACTCGGAGAAGATTCTGCTCAGTCTGCGCGAGATTATTCGCGAGG
AGGACCAGAACACCCGCGTCACCTTGCTGGAGTACCTCAAGCAGCTCCACCCT
GTCGAATGGGACAACTTCGTCAAGGACACCAAGATCTTGGCGGAAGAGTCGGG
CGACGTCCAGGACGAGAAGCGCGCGCGCACGGACGACTTGCCGTTCTACTGC
ATCGGGTTCAAGACCTCGTCACCAGAGTACACCCTGCGTACGCGTATCTGGGC
TTCACTGCGCGCACAGACGCTGTACCGCACGGTCTCCGGTATGATGAACTACTC
CAAGGCGATCAAGCTCCTCTATCGCGTCGAGAACCCGGATGTCGTTCATGCCTT
CGGTGGGAACACGGAACGTCTTGAACGCGAGCTTGAGCGCATGTCTCGCCGCA
AGTTCAAGTTCGTCATCTCGATGCAGCGGTACTCTAAGTTCAACAAGGAGGAGC
AAGAGAACGCCGAATTCCTTCTGCGCGCGTACCCGGATTTGCAGATCGCGTAC
CTCGATGAAGAGCCCGGTCCCAGCAAGAGCGACGAGGTTCGGTTGTTTTCGAC
ACTCATCGATGGACACTCCGAGGTGGATGAGAAGACCGGCCGCCGCAAGCCCA
AGTTCCGCATTGAGCTGCCCGGTAACCCCATCCTCGGTGACGGGAAGTCGGAT
AACCAGAACCACGCCATTGTCTTCTACCGCGGCGAGTACATCCAGGTCATCGAC
GCTAACCAGGACAATTACCTGGAAGAGTGTCTCAAGATCCGTAACGTCCTGGGC
GAGTTTGAGGAATACTCCGTGTCGAGCCAGAGCCCGTACGCACAGTGGGGCCA
CAAGGAGTTCAACAAGTGCCCCGTCGCTATCCTGGGTTCTCGCGAGTACATCTT
CTCGGAGAACATCGGTATCCTCGGTGACATCGCCGCCGGCAAGGAACAGACGT
TCGGTACCATTACGGCGCGTGCGCTTGCGTGGATCGGCGGCAAGCTGCATTAC
GGTCACCCGGATTTCCTCAATGCGACGTTCATGACGACGCGTGGTGGCGTGTC
AAAAGCGCAGAAGGGCTTGCATCTCAACGAGGATATCTTCGCTGGTATGACCGC
CGTGTCCCGCGGAGGGCGCATCAAGCACATGGAGTACTACCAGTGCGGCAAAG
GTCGTGATCTCGGTTTCGGCACGATCTTGAACTTCCAGACGAAGATCGGTACTG
GTATGGGCGAGCAGCTCCTCTCGCGCGAGTACTACTACCTGGGCACGCAATTG
CCTATCGACCGGTTCTTGACGTTCTACTACGCGCACGCTGGTTTCCACGTCAAC
AACATCCTGGTCATCTACTCCATCCAGGTCTTCATGGTCACCTTGCTGTACCTG
GGCACATTGAACAAGCAGCTGTTCATCTGCAAGGTCAACTCCAATGGCCAGGTT
CTTAGTGGACAAGCTGGGTGCTACAACCTCATCCCGGTCTTCGAGTGGATTCGC
CGGAGTATCATCTCCATCTTCTTGGTGTTCTTCATCGCCTTCTTGCCTCTATTCTT
GCAAGAGCTGTGCGAGCGCGGAACGGGAAAGGCGTTGCTGCGTCTCGGGAAG
CACTTCTTGTCACTGTCGCCCATTTTCGAAGTGTTCTCCACCCAGATTTACTCGC
AGGCGCTCTTGAACAACATGAGCTTCGGTGGTGCGCGCTACATCGCCACAGGT
CGTGGTTTCGCGACTAGTCGCATACCCTTCAACATCCTCTACTCGCGTTTCGCG
CCGCCAAGCATCTACATGGGCATGCGTAACCTGCTGCTCCTGCTGTACGCGAC
GATGGCCATTTGGATCCCGCACCTGATCTACTTCTGGTTCTCCGTCCTCTCCCT
CTGCATCGCGCCATTCATGTTCAATCCGCATCAATTCTCGTACGCCGACTTCATC
ATCGACTACCGGGAGTTCTTGCGCTGGATGTCGCGCGGTAACTCGCGAACGAA
GGCGAGCAGCTGGTACGGATACTGCCGTCTGTCGCGTACCGCGATTACTGGGT
ACAAGAAGAAGAAGCTGGGACACCCGTCGGAGAAGCTGTCGGGCGACGTACC
GCGTGCGCCGTGGAGGAACGTTATCTTCTCGGAGATCCTGTGGCCCATCGGCG
CGTGCATCATCTTCATCGTCGCGTACATGTTCGTCAAGTCGTTCCCCGACGAGC
AGGGCAACGCGCCGCCGAGCCCGCTGGTCCGGATTCTGCTCATCGCGGTTGG
CCCTACTGTGTGGAACGCGGCGGTGCTCATAACGCTGTTCTTCCTGTCGCTCTT
CCTGGGCCCGATGATGGATGGCTGGGTCAAGTTCGGCTCGGTCATGGCGGCC
CTTGCGCATGGCCTGGCGCTTATAGGCATGCTCACGTTCTTTGAGTTCTTCTGG
TTCCTTGAGCTCTGGGATGCCTCGCACGCCGTGCTCGGCGTCATCGCTATCATT
GCCGTTCAGCGCGGGATCCAGAAGATCCTCATTGCCGTCTTCCTGACGCGTGA
GTACAAGCACGACGAGACGAACCGCGCGTGGTGGACAGGTAAATGGTATGGAC
GCGGGCTGGGTACCTCGGCCATGTCCCAGCCGGCGCGCGAGTTCATCGTGAA
GATCGTGGAGATGTCGTTGTGGACGTCGGACTTCCTGCTTGCGCACCTGTTGCT
CATCATCTTGACGGTGCCGCTACTGCTGCCGTTCTTCAACTCAATTCATTCGAC
GATGCTTTTCTGGTTGCGCCCTTCGAAGCAGATTAGGCAACCTCTGTTCTCCAC
CAAGCAGAAGCGGCAACGGCGATGGATTGTCATGAAGTATACCGTGGTATATCT
CGTGGTGGTGGCTTTCCTCGTCGCGCTCATCGCTCTGCCCGCCCTCTTCCGCG
AGAGCATCCACTTCAACTGCGAGATCTGCCAGAGTATATAG polypeptide sequence
1,3-13-D-glucan synthase I of S. commune strain Lu15634 amino acid
S. commune SEQ ID NO: 14
MSGPGYGRNPFDNPPPNRGPYGQQPGFPGPGPRPYDSDADMSQTYGSTTRLAG
SAGYSDRNGSFDGDRSYAPSIDSRASVPSISPFADPGIGSNEPYPAWSVERQIPMS
TEEIEDIFLDLTQKFGFQRDSMRNTFDFMMHLLDSRASRMTPNQALLTLHADYIGGQ
HANYRKWYFAAQLNLDDAVGQTNNPGIQRLKTIKGATKTKSLDSALNRWRNAMNN
MSQYDRLRQIALYLLCWGEAGNIRLAPECLCFIFKCADDYYRSPECQNRMDPVPEG
LYLQTVIKPLYRFLRDQAYEVVNDGKQVKREKDHDQIIGYDDVNQLFWYPEGLAKIVM
SDNTRLVDVPPAQRFMKFAKIEWNRVFFKTYFEKRSTAHLLVNFNRIWILHVSMYFF
YTAFNSPRVYAPHGKLDPSPEMTWSATALGGAVSTMIMILATIAEYTYIPTTWNNAS
HLTTRLIFLLVILALTAGPTFYIAMIDGRTDIGQVPLIVAIVQFFISVVATLAFATIPSGRM
FGDRVAGKSRKHMASQTFTASYPSMKRSSRVASIMLWLLVFGCKYVESYFFLTSSF
SSPIAVMARTKVQGCNDRIFGSQLCTNQVPFALAIMYVMDLVLFFLDTYLWYIIWLVI
FSMVRAFKLGISIWTPWSEIFTRMPKRIYAKLLATAEMEVKYKPKVLVSQIWNAVIISM
YREHLLSIEHVQRLLYHQVDGPDGRRTLRAPPFFTSQRTAKPGLFFPPGGEAERRIS
FFASSLTTALPEPLPIDAMPTFTVLVPHYSEKILLSLREIIREEDQNTRVTLLEYLKQLH
PVEWDNFVKDTKILAEESGDVQDEKRARTDDLPFYCIGFKTSSPEYTLRTRIWASLR
AQTLYRTVSGMMNYSKAIKLLYRVENPDVVHAFGGNTERLERELERMSRRKFKFVI
SMQRYSKFNKEEQENAEFLLRAYPDLQIAYLDEEPGPSKSDEVRLFSTLIDGHSEVD
EKTGRRKPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYIQVIDANQDNYLEECLKIR
NVLGEFEEYSVSSQSPYAQWGHKEFNKCPVAILGSREYIFSENIGILGDIAAGKEQTF
GTITARALAWIGGKLHYGHPDFLNATFMTTRGGVSKAQKGLHLNEDIFAGMTAVSR
GGRIKHMEYYQCGKGRDLGFGTILNFQTKIGTGMGEQLLSREYYYLGTQLPIDRFLT
FYYAHAGFHVNNILVIYSIQVFMVTLLYLGTLNKQLFICKVNSNGQVLSGQAGCYNLI
PVFEWIRRSIISIFLVFFIAFLPLFLQELCERGTGKALLRLGKHFLSLSPIFEVFSTQIYS
QALLNNMSFGGARYIATGRGFATSRIPFNILYSRFAPPSIYMGMRNLLLLLYATMAIW
IPHLIYFWFSVLSLCIAPFMFNPHQFSYADFIIDYREFLRWMSRGNSRTKASSWYGY
CRLSRTAITGYKKKKLGHPSEKLSGDVPRAPWRNVIFSEILWPIGACIIFIVAYMFVKS
FPDEQGNAPPSPLVRILLIAVGPTVWNAAVLITLFFLSLFLGPMMDGVVVKFGSVMAA
LAHGLALIGMLTFFEFFWFLELWDASHAVLGVIAIIAVQRGIQKILIAVFLTREYKHDET
NRAVWVTGKWYGRGLGTSAMSQPAREFIVKIVEMSLWTSDFLLAHLLLIILTVPLLLP
FFNSIHSTMLFWLRPSKQIRQPLFSTKQKRQRRWIVMKYTVVYLVVVAFLVALIALPA
LFRESIHFNCEICQSI cDNA 1,3-.beta.-D-g I ucan synthase II of S.
commune strain Lu15634 DNA S. commune SEQ ID NO: 15
ATGCCGAGGCCGGGCGGCACCAGCGCAGAAGGCGGCTACGCATCATCGCCGT
CGATGGAGACGACCCCCAGCGATCCCTTCGGAACCGCGAACGGCGCGCCCCG
CCGCTACTACGACAATGATTCTGAGGAGTACGGACCTGGCCGTAGAGACACCT
ACGCGTCCGACAGCAGTAATCAGGGCCTCACGGACCCGGGCTACTACGACCAG
AATGGCGCCTATGATCCCTATCCGACCGGGGACACCGATTCCGACGGCGACGT
CTACGGCCAGCGATATGGACCCTCAGCAGAGTCGCTTGGCACCCACAAGTTCG
GCCATTCCGATTCATCCACGCCGACTTTTGTCGACTACAGCGCATCCTCCGGCG
GGAGGGATTCGTACCCTGCATGGACTGCCGAACGCAACATCCCGCTGTCCAAG
GAGGAGATCGAGGACATCTTCCTCGATTTGACGCAGAAGTTTGGCTTTCAGCGG
GATTCCATGCGGAATATGTTCGACTTCACCATGCAGCTGCTTGACAGCCGAGCG
TCTCGTATGACCCCCAACCAGGCGCTCCTCACCCTCCACGCCGACTACATTGGT
GGCCAGCATGCGAACTACCGGAAGTGGTACTTCGCGGCGCAGCTCGACCTTGA
CGACGCCGTGGGACAAACTCAGAATCCGGGTCTCAACCGCCTCAAGTCCACTC
GCGGATCGGGCAAGCGACCACGCCATGAAAAGTCGCTGAACACGGCATTGGAG
CGCTGGCGGCAAGCCATGAACAACATGTCGCAGTATGACCGCTTACGCCAGAT
CGCGCTCTACCTGCTCTGCTGGGGCGAAGCGGCGCAAGTGCGATTCATGCCCG
AGTGCTTGTGCTTCATCTTCAAGTGCGCCGACGACTACTATCGTTCGCCGGAGT
GCCAGAACAGGATGGAGCCGGTACCGGAGGGTCTCTACCTGAGGACGGTCGT
AAAGCCGCTCTACAGATTTGTCCGGGATCAAGGCTATGAGGTGGTGGAGGGAA
AATTCGTACGGCGGGAACGGGATCACGACCAAATCATTGGTTACGATGACGTGA
ATCAGCTGTTCTGGTACCCGGAGGGAATTGCCCGTATCGTCCTGTCGGACAAG
AGTCGTCTAGTCGACCTCCCCCCAGCACAGCGCTTCATGAAGTTCGACCGTATC
GAGTGGAATCGCGTCTTCTTCAAGACGTTTTACGAGACTCGATCCTTCACGCAT
CTTTTGGTCGACTTCAACCGTATCTGGGTCGTGCACATCGCTCTCTACTTCTTCT
ACACTGCATACAACTCCCCCACGATCTACGCCATCAACGGCAACACACCGACGT
CTCTGGCTTGGAGCGCGACTGCGCTCGGCGGTGCGGTAGCGACAGGTATCAT
GATCCTCGCCACGATCGCCGAGTTCTCGCACATCCCCACGACATGGAACAACA
CCTCGCATCTGACTCGCCGCCTCGCCTTCCTCCTCGTCACGCTCGGCCTCACAT
GTGGTCCGACGTTCTACGTCGCGATTGCAGAGAGCAACGGGAGCGGCGGCTCT
TTGGCCTTGATTCTCGGTATCGTCCAGTTCTTCATCTCCGTCGTGGCAACTGCG
CTCTTCACTATCATGCCTTCTGGTCGTATGTTCGGCGACCGTGTCGCAGGCAAG
AGTCGCAAGTATCTCGCCAGCCAGACGTTCACGGCCAGCTACCCGTCGTTGCC
CAAGCACCAGCGGTTCGCCTCACTCCTGATGTGGTTCCTCATCTTCGGGTGCAA
GTTGACGGAGAGTTACTTCTTTCTGACGCTGTCCTTCCGCGACCCTATCCGCGT
CATGGTCGGCATGAAGATCCAGAACTGCGAGGACAAGATTTTCGGCAGCGGCC
TTTGCAGGAATCACGCAGCATTCACCCTCACGATCATGTACATCATGGACCTCG
TCTTGTTCTTCCTCGACACCTTCCTTTGGTATGTCATCTGGAACTCGGTTTTCAG
TATCGCACGCTCTTTCGTACTCGGCCTTTCGATCTGGACACCGTGGAGAGACAT
CTTCCAGCGTCTGCCGAAGCGGATCTACGCGAAGCTTCTGGCGACTGGCGACA
TGGAGGTCAAGTACAAGCCCAAGGTCTTGGTCTCGCAAATCTGGAACGCCATCA
TCATCTCCATGTACCGCGAGCACTTGCTCTCTATTGAGCACGTCCAGAAGCTCC
TGTACCACCAAGTGGACACTGGCGAAGCCGGCAAGCGGAGTCTTCGCGCGCCT
CCGTTCTTCGTCGCGCAGGGCAGCAGCGGTGGCTCGGGCGAGTTCTTCCCGC
CTGGCAGCGAGGCCGAGCGTCGTATCTCTTTCTTCGCGCAGTCGCTTTCTACG
GAGATTCCTCAGCCCATCCCGGTCGACGCCATGCCGACGTTCACGGTGCTTAC
GCCTCACTACAGCGAGAAGATCCTTCTCTCTCTCCGTGAAATTATCCGCGAGGA
GGACCAGAACACTCGCGTTACGTTGCTCGAGTACCTGAAGCAGCTGCATCCGG
TCGAGTGGGAGAATTTCGTCAAGGACACTAAAATTTTGGCCGAGGAGTCCGCTA
TGTTTAACGGTCCGAGTCCTTTCGGCAACGACGAGAAGGGTCAGTCCAAGATG
GACGATCTACCGTTCTACTGCATCGGTTTCAAGAGCGCCGCGCCCGAGTACAC
CCTCCGCACCCGTATCTGGGCGTCCCTGCGCGCGCAGACGCTGTACCGCACG
GTCTCCGGCATGATGAACTATGCGAAGGCGATCAAGCTGCTCTACCGCGTTGA
GAACCCGGAGGTCGTACAACAGTTCGGCGGCAACACGGACAAGCTCGAGCGC
GAGTTGGAGCGGATGGCGCGACGGAAGTTCAAGTTCCTCGTGTCCATGCAGCG
CTACTCGAAGTTCAACAAGGAGGAGCACGAGAACGCCGAGTTCTTGCTCCGCG
CGTACCCGGACTTGCAGATCGCGTACCTCGAGGAAGAGCCCCCTCGCAAGGAG
GGCGGCGATCCACGCATCTTCTCTGCCCTCGTCGACGGCCACAGCGACATCAT
CCCGGAGACCGGCAAGCGGCGCCCCAAGTTCCGTATCGAGCTGCCCGGTAAC
CCCATTCTCGGTGACGGTAAATCCGACAATCAGAACCACGCTATCGTCTTCTAC
CGCGGCGAGTACCTCCAGCTTATCGACGCCAACCAGGACAACTACCTCGAGGA
GTGCTTGAAGATCCGTAACGTGCTCGCCGAGTTTGAGGAGTACGACGTCTCCA
GCCAGAGCCCGTACGCGCAGTGGAGTGTCAAGGAGTTCAAGCGCTCTCCGGTC
GCCATCGTCGGTGCACGCGAGTACATCTTCTCAGAGCACATCGGTATCCTCGGT
GATCTGGCGGCTGGCAAGGAACAGACGTTCGGTACGCTCACGGCACGCAACAA
CGCCTTCCTTGGCGGCAAGCTGCACTACGGTCACCCCGATTTCCTCAACGCCC
TCTACATGAACACGCGCGGTGGTGTCTCCAAGGCGCAGAAGGGTCTCCATCTC
AACGAGGATATCTACGCCGGTATGAACGCGGTCGGTCGCGGTGGACGCATTAA
GCACAGCGAGTACTATCAGTGCGGCAAGGGTCGTGACCTCGGTTTCGGCACCA
TCTTGAACTTCCAGACCAAGATCGGTACGGGTATGGGCGAGCAGATCCTCTCG
CGCGAGTACTACTATCTCGGAACACAACTGCCCATCGATCGCTTCCTCACGTTC
TACTACGCGCACCCGGGTTTCCAGATCAACAACATGCTGGTCATCCTCTCCGTG
CAGGTCTTCATCGTTACCATGGTCTTCCTCGGTACCTTGAAGTCTTCGGTCACG
ATCTGCAAGTACACGTCCAGCGGTCAGTACATCGGTGGTCAATCCGGTTGCTAC
AACCTCGTCCCGGTCTTCCAGTGGATCGAGCGCTGCATCATCAGCATCTTCTTG
GTGTTCATGATCGCTTTCATGCCGCTCTTCCTGCAAGAACTCGTCGAGCGCGGT
ACCTGGAGTGCCATCTGGCGTCTGCTCAAGCAGTTTATGTCGCTGTCGCCTGTC
TTCGAGGTGTTCTCCACCCAGATTCAGACGCACTCCGTGTTGAGCAACTTGACG
TTCGGTGGTGCGCGTTACATCGCTACCGGTCGTGGGTTCGCCACCAGTCGTAT
CAGCTTCAGCATCTTGTTCTCGCGTTTCGCAGGCCCGAGTATCTACCTCGGCAT
GCGCACGCTCATTATGCTGCTCTACGTGACGTTGACGATCTGGACGCCATGGG
TCATTTACTTCTGGGTTTCCATTCTCTCGCTCTGCATCGCGCCGTTCTTGTTCAA
CCCGCATCAATTCGTATTCTCGGACTTCCTCATCGACTACAGGGAATACCTGCG
GTGGATGTCGCGTGGCAACTCGCGCTCGCACAACAACTCCTGGATTGGGTACT
GCCGGTTGTCCCGCACGATGATCACTGGGTACAAGAAGAAGAAGCTGGGCCAC
CCGTCGGAGAAGCTTTCCGGCGACGTTCCTCGTGCAGGCTGGCGCGCCGTCTT
GTTCTCGGAGATCATCTTCCCGGCGTGCATGGCCATCCTCTTCATCATCGCGTA
CATGTTCGTCAAGTCGTTCCCTCTCGACGGCAAGCAGCCTCCCTCCGGCCTCG
TTCGCATCGCCGTCGTGTCTATCGGCCCCATCGTGTGGAACGCCGCCATCCTG
TTGACGCTCTTCCTTGTGTCGTTGTTCCTCGGCCCCATGCTCGACCCGGTCTTC
CCCCTCTTCGGTTCCGTTATGGCCTTCATCGCGCATTTCCTTGGCACAATCGGA
ATGATTGGGTTCTTCGAGTTCCTGTGGTTCCTCGAGTCCTGGGAGGCGTCGCAT
GCCGTGCTGGGTCTCATCGCCGTCATCTCCATCCAGCGCGCCATTCACAAGAT
CCTTATCGCCGTTTTCCTCAGTCGCGAGTTCAAGCACGACGAGACGAACAGGG
CCTGGTGGACTGGTCGCTGGTATGGCCGTGGCCTCGGCACGCACGCCATGTC
GCAGCCGGCGCGTGAGTTCGTCGTCAAGATCATCGAGTTGTCGCTTTGGAGCT
CGGATCTCATACTCGGCCACATCCTGCTGTTCATGCTTACTCCGGCCGTCCTCA
TCCCGTACTTCGACCGTTTGCACGCCATGATGCTCTTCTGGCTGCGTCCCTCGA
AGCAAATCCGCGCGCCTCTGTACTCGATCAAGCAGAAGAGGCAAAGACGCTGG
ATTATCATGAAGTACGGTACTGTATACGTTACCGTCATCGCGATCTTCGTCGCG
CTCATCGCGCTTCCCCTCGTATTCCGACACACTCTAAAGGTCGAGTGCTCCCTT
TGCGACAGCTTGTAA polypeptide sequence 1,3-.beta.-D-glucan synthase
II of S. commune strain Lu15634 amino acid S. commune SEQ ID NO: 16
MPRPGGTSAEGGYASSPSMETTPSDPFGTANGAPRRYYDNDSEEYGPGRRDTYA
SDSSNQGLTDPGYYDQNGAYDPYPTGDTDSDGDVYGQRYGPSAESLGTHKFGHS
DSSTPTFVDYSASSGGRDSYPAWTAERNIPLSKEEIEDIFLDLTQKFGFQRDSMRN
MFDFTMQLLDSRASRMTPNQALLTLHADYIGGQHANYRKVVYFAAQLDLDDAVGQT
QNPGLNRLKSTRGSGKRPRHEKSLNTALERWRQAMNNMSQYDRLRQIALYLLCW
GEAAQVRFMPECLCFIFKCADDYYRSPECQNRMEPVPEGLYLRTVVKPLYRFVRD
QGYEWEGKFVRRERDHDQIIGYDDVNQLFWYPEGIARIVLSDKSRLVDLPPAQRF
MKFDRIEWNRVFFKTFYETRSFTHLLVDFNRIWVVHIALYFFYTAYNSPTIYAINGNT
PTSLAWSATALGGAVATGIMILATIAEFSHIPTTWNNTSHLTRRLAFLLVTLGLTCGPT
FYVAIAESNGSGGSLALILGIVQFFISVVATALFTIMPSGRMFGDRVAGKSRKYLASQ
TFTASYPSLPKHQRFASLLMWFLIFGCKLTESYFFLTLSFRDPIRVMVGMKIQNCED
KIFGSGLCRNHAAFTLTIMYIMDLVLFFLDTFLWYVIWNSVFSIARSFVLGLSIWTPWR
DIFQRLPKRIYAKLLATGDMEVKYKPKVLVSQIWNAIIISMYREHLLSIEHVQKLLYHQ
VDTGEAGKRSLRAPPFFVAQGSSGGSGEFFPPGSEAERRISFFAQSLSTEIPQPIPV
DAMPTFTVLTPNYSEKILLSLREIIREEDQNTRVTLLEYLKQLHPVEWENFVKDTKILA
EESAMFNGPSPFGNDEKGQSKMDDLPFYCIGFKSAAPEYTLRTRIWASLRAQTLYR
TVSGMMNYAKAIKLLYRVENPEVVQQFGGNTDKLERELERMARRKFKFLVSMQRY
SKFNKEEHENAEFLLRAYPDLQIAYLEEEPPRKEGGDPRIFSALVDGHSDIIPETGKR
RPKFRIELPGNPILGDGKSDNQNHAIVFYRGEYLQLIDANQDNYLEECLKIRNVLAEF
EEYDVSSQSPYAQWSVKEFKRSPVAIVGAREYIFSEHIGILGDLAAGKEQTFGTLTA
RNNAFLGGKLHYGHPDFLNALYMNTRGGVSKAQKGLHLNEDIYAGMNAVGRGGRI
KHSEYYQCGKGRDLGFGTILNFQTKIGTGMGEQILSREYYYLGTQLPIDRFLTFYYA
HPGFQINNMLVILSVQVFIVTMVFLGTLKSSVTICKYTSSGQYIGGQSGCYNLVPVFQ
WIERCIISIFLVFMIAFMPLFLQELVERGTWSAIWRLLKQFMSLSPVFEVFSTQIQTHS
VLSNLTFGGARYIATGRGFATSRISFSILFSRFAGPSIYLGMRTLIMLLYVTLTIWTPW
VIYFWVSILSLCIAPFLFNPHQFVFSDFLIDYREYLRWMSRGNSRSHNNSWIGYCRL
SRTMITGYKKKKLGHPSEKLSGDVPRAGWRAVLFSEIIFPACMAILFIIAYMFVKSFPL
DGKQPPSGLVRIAVVSIGPIVVVNAAILLTLFLVSLFLGPMLDPVFPLFGSVMAFIAHFL
GTIGMIGFFEFLWFLESWEASHAVLGLIAVISIQRAIHKILIAVFLSREFKHDETNRAW
WTGRVVYGRGLGTHAMSQPAREFVVKIIELSLWSSDLILGHILLFMLTPAVLIPYFDRL
HAMMLFWLRPSKQIRAPLYSIKQKRQRRWIIMKYGTVYVTVIAIFVALIALPLVFRHTL
KVECSLCDSL teff promoter DNA S. commune SEQ ID NO: 17
ATCGCCATTGTAAGCCGCAGACGGGCACGCTTCCAACCCCCATCGATGGGCGC
TCGATGTCCATCTCATCGGCGACTCATCATTGTATCTCGCGCAGTCCCATCCCT
CGCCGCTCGCCTGTAGTTTATGCTATTTATCTTTGCACCAGTCGTTGTATTACTC
CCTCGTCGTGTAGAAAGTACCAGATAAAATGCATGTAATCCTAATGAAATTTGCA
CGACACGAAGATCCGGCAGGGTTGTGGGCAAGGGGCAGCGGGAACGAATGGA
TGGCGGGGTACAGCGAGTACCCGGCAGTGCCACAGTCAGTGTCACACACGTGA
CTGATTGTCCATTAGCGTGACCGATAACATCGATCAAAAATTTTATTTCAGAGGA
CGATAAATAAGGGCCGACGGTGCGCGTCCGTCTTTCTCTCAACCCTCATCTTCC
TCTCGTCTCTCACTCTTCCCCCCTCCACCACTACCAAGTAAGTTCAAACTTCCTC
TCATCGCCTTTGCACACATCGCCTACGCCCCATCTCTCTCCATCTGCCTCGCGA
ACGGCGCCCCCATCGTCGCTTTCCCGCGCGAGATCTTGTGCGATCTAGTTTACT
GACAATCTCACCTAGAAAACATCAAA teff terminator DNA S. commune SEQ ID
NO: 18 ATCCAAGTCCGGTGGCAAGGTCACCAAGTCCGCCGAGAAGGCCGCCAAGAAGA
AGTAAATGTAGATGTACATATGTATTTTCTCATTCCGTTTCCTTCCTCTTGTTGTT
GTTTCACTGGTCCTCTCGTGCTCGCTCGCATCGCATACAGCCATTGTTGTCACC
ACTATAACTTCACGCATTCTGTATTTCATGCCAGGCGACGGGGTGTTCCTGCCA
GGCCTGTCGCTTGTTGTAACGCTAATGAAAAGTCACGAGTAGTGGACGAACGAC
GATGTATTTCTATGTGCTGTAGCGATTATCCATTTCGAGTTCGCCATCGAGCTCT
CTTCAAACCTAGGTGCGACGTTGTGAATGCAGTAGCAAGTGCAGAGTATTGCAG
ACTCGTCCATTGATGATAACTTCAAGCTACGTCAGAGCCAGATGCTACTGAACC CGGGCC
Ura_forw (NotI) primer DNA artificial SEQ ID NO: 19
ATAAGAATGCGGCCGCTCCAGCTCGACCTTGCGCCG Ura_rev (XbaI) primer DNA
artificial SEQ ID NO: 20 CTAGTCTAGAGGATCCGACGTGGAGGAGCC
TefP_forw (XbaI) primer DNA artificial SEQ ID NO. 21
CTAGTCTAGAATCGCCATTGTAAGCCGCAG TefP_rev (SpeI) primer DNA
artificial SEQ ID NO: 22 CTAGACTAGTTTTGATGTTTTCTAGGTGAG TefT_forw
(Sall) primer DNA artificial SEQ ID NO: 23
ACGCGTCGACCAAGTCCGGTGGCAAGGTCA TefT_rev (SalI) primer DNA
artificial SEQ ID NO: 24 CCGACGTCGACGGGTTCAGTAGCATCTGGCT TefT_forw
(EcoRV) primer DNA artificial SEQ ID NO: 25
CATGGTGATATCCAAGTCCGGTGGCAAGGTCA TefT_rev (ApaI) primer DNA
artificial SEQ ID NO: 26 CCGTATGGGCCCGGGTTCAGTAGCATCTGGCT GS1_forw
(SpeI) primer DNA artificial SEQ ID NO: 27
CTAGACTAGTCCCGTCCCTCAAGGCCGTTC GS1_rev (SalI) primer DNA artificial
SEQ ID NO: 28 AATGGCCGACGTCGACATGGTATATGCAATGCTATG Fusion
TefP_GS1_forw (XbaI) primer DNA artificial SEQ ID NO: 29
CTAGTCTAGAATCGCCATTGTAAGCCGCAG Fusion TefP_GS1_rev (SalI) primer
DNA artificial SEQ ID NO: 30 AATGGCCGACGTCGACATGGTATATGCAATGCTATG
GS2 forw (SpeI) primer DNA artificial SEQ ID NO: 31
CTAGACTAGTCTGTCCAAAGAAGAGATCGA GS2_rev (EcoRV) primer DNA
artificial SEQ ID NO: 32 TACATGCGATATCTTTTATGCAGACTCTCCCTG ura gene
DNA S. commune SEQ ID NO: 33
TCCAGCTCGACCTTGCGCCGCTTGGAGTAACGTTCAGCGTCTTCGTCGTCCTCG
TCGCGCTCGTGTACGATGATGGGCTCAGCCATGGCAGGTATACAAGCTCAGAG
TCAATGGGGGACGAGGTCTCAAGCCGTGAAAGTCGTCGTCGAACAACGTCAAG
TTCGAGACGGACCAGAGTTGGATTTCGTGATTAGATCTACGCTCGATCACAGAA
TGATCAAAGAACAAAGCTTGCCAAAAGGGGATCTCCCATCAACTTCAACTTGCC
CCAAACCATCATGACCGCCGCTCATAAGCTCACATACGGTCAGCGCGCTGCAA
GGTTCACCAATCCCGCGGCGAAAGCCCTGCTGGAAACCATGGAGCGCAAGAAG
AGCAATCTATCCGTCAGCGTCGACGTCGTAAAATCCGCCGATCTGCTCGCTATT
GTCGATACCGTCGGGCCCTATATCTGTCTGATAAAGGCATTGCACTGTCGCTTG
CGGTCTTGGGATGCTGCTTATACTCTATGAAGACCCATGTGGATGTTGTCGAAG
ACTTCGACTCGTCGCTCGTCACCAAGCTTCAGGCTCTGGCCGAGAAGCATGATT
TCCTCATCTTTGAGGACAGAAAATTCGCCGACATAGGTCTGTCCGTCGAATCTC
TATCGATGTCAACTCTGATGACTTGCACAGGCAACACCGTCGCTCTGCAGTACT
CTAGTGGCGTGCACAAAATTGCCAGCTGGTCGCACATCACGAACGCACACCCT
GTTCCAGGACCGTCAATCATCAGTGGCCTCGCATCGGTAGGACAACCCCTCGG
TCGCGGACTCCTCCTGCTCGCAGAGATGAGCACGAAGGGCTCACTTGCGACAG
GCGCGTACACTGAAGCCGCCGTCCAGATGGCAAGGGAGAACCGCGGCTTCGT
CATCGGGTTCATCGCCCAACGGCGGATGGATGGTATTGGCGCGCCTCCAGGG
GTGAATGTCGAGGACGAGGATTTTCTTGTCTTGACACCAGGTGTCGGACTCGAT
GTGAAGGGCGATGGGATGGGGCAGCAATACAGGACGCCGAAGCAAGTGGTAC
AGGAAGATGGGTGCGATGTAATCATCGTGGGTCGCGGGATTTATGGCAAGGAC
CCATCGAAGGTGGAAGAGATACGGAGGCAGGCAGAGCGTTACCAGGCTGCAG
GATGGGCGGCGTACATTGAGAGGGTCAACGCCTTGGTATAGCTAATCTGATCG
GTGTTGTCTTGTTAAGCGTCAGGCTCAATGGAACGCTTTGGACGAGCGGAGAGT
AACTTGAATTAGCAGTGTATACTTCGGGCAAATCAATCGTGATAAATACAAGAGC
ACGCTCACGCACGTCCAATCTCCCTCAAAATCTCCATCTTTCTCGCCTCATTCAC
CTTCCTGAACCCAGCCGGCGACATCTCGAACAGACCATGCCCACCCGACAGCG
CACGCAGCCTATTCGAGTAGTCCAGCATCCGGCTGAGCGGCGCCACCGCCTGC
ACCGCGCGCTTCATCTTCACGCCCGCCGCCTCCCTCGCCGCAGTGCCGCCAGA
GGGCGACACCCACTCCGGGGGCACGTACACGCCGTCCGCAGGGTACGGCTCC TCCACGTCGGATCC
Ura protein amino acid S. commune SEQ ID NO: 34
MTAAHKLTYGQRAARFTNPAAKALLETMERKKSNLSVSVDVVKSADLLAIVDTVGPY
ICLIKTHVDVVEDFDSSLVTKLQALAEKHDFLIFEDRKFADIGNTVALQYSSGVHKIAS
WSHITNAHPVPGPSIISGLASVGQPLGRGLLLLAEMSTKGSLATGAYTEAAVQMARE
NRGFVIGFIAQRRMDGIGAPPGVNVEDEDFLVLTPGVGLDVKGDGMGQQYRTPKQ
WQEDGCDVIIVGRGIYGKDPSKVEEIRRQAERYQAAGWAAYIERVNALV
Sequence CWU 1
1
3416100DNASchizophyllum commune 1cccgtccctc aaggccgttc tttcgctggc
gaccgacccg gtgttcgcga gaacctgttg 60tttctgacga tcatcagccc tttcttctcg
tcgctcttta gctctcccta gaccgtcttt 120tactctactc ttcgacgcac
gccatgtccg gcccaggata tggcaggaat ccattcgaca 180atcccccgcc
caacagaggt ccctatggcc agcagccagg tttcccgggg cccggccctc
240ggccttacga ctcggacgcg gacatgagcc agacctatgg cagcacaacc
aggctcgccg 300gcagtgccgg ttacagcgac agaaacggtg cgcacgtcgc
taccgtactt cctcgatcgt 360cgattcacat accatgcagg cagcttcgac
ggcgaccgct cctacgcgcc ctcaattgac 420tcgcgcgcca gcgtgcccag
catatcgccc ttcgcagacc cgggtatcgg ctctaatgag 480ccgtatcccg
cttggtcggt cgaacgccag attcccatgt ccacggagga gattgaggac
540atcttcctcg acctcaccca aaagtttggc ttccagcgcg actccatgcg
gaatacggtg 600cgtgaataag cagcccactc gaccgcggga acagcacaat
tgacctgtca cccagttcga 660cttcatgatg cacctcctcg attcccgtgc
ctcgcgcatg acgcccaacc aagctctgct 720cacgcttcac gccgactaca
ttggtggcca gcatgccaat taccggaagt ggtatttcgc 780cgcacagctc
aacctcgatg acgcggtcgg gcaaaccaat aaccccggta tccagcgctt
840gaagaccatc aagggcgcta cgaagaccaa gtcgctcgac agcgcactca
accgctggcg 900caacgcgatg aacaacatga gccagtacga tcgcctccgg
caaattgcgc tctacctcct 960ctgctggggt gaagcaggca acatccgtct
ggcgcccgag tgcttgtgct tcatcttcaa 1020gtgcgcggac gactactaca
gaagtcccga gtgtcagaac cggatggacc ccgtgccgga 1080agggctgtac
ctgcagacgg tcatcaagcc gctctatcgc ttcctacgtg atcaggcgta
1140cgaagtcgtt gatgggaagc aagtgaagcg cgagaaggac cacgaccaga
ttatcggtta 1200tgacgacgtc aaccagttat tctggtatcc ggaaggtttg
gctaagatcg tcatgtcgga 1260caacgtgcgt atgatcttat cggttaaaat
tcgtccgctc acatctttcc agacacgact 1320tgtagatgta cctccggcgc
agcggttcat gaagttcgcc aagatcgagt ggaaccgcgt 1380cttcttcaag
acgtactttg agaagcgctc tactgcccat ctcctggtca acttcaaccg
1440tatatggatc ctccacgtct cgatgtactt cttctacacg gcattcaact
ctccacgagt 1500ctacgcgccg cacggcaaac tcgacccctc ccctgagatg
acctggtccg cgactgccct 1560tggaggcgct gtgtccacca tgatcatgat
ccttgccact atcgcggagt acacctacat 1620ccccacgaca tggaacaatg
cgtcgcacct caccacgcgg ctcattttcc tcctggtcat 1680cctcgcgctc
actgctggcc caacattcta tatcgccatg atagacggac gcacggacat
1740cggccaagta ccactcatcg tggccatagt gcagttcttc atctccgtcg
tcgccaccct 1800cgctttcgct accatccctt ctggtcgcat gttcggcgac
cgtgtggctg gcaagtcaag 1860aaagcacatg gcatcgcaga cgttcacagc
gtcgtacccg tccatgaagc ggtcatctcg 1920cgtagcgagt atcatgctgt
ggcttttggt ctttggctgc aaatacgtcg agtcttactt 1980cttcttgacg
tcctccttct ccagcccgat cgcggtcatg gcgcgtacga aggtacaggg
2040ctgcaacgac cgtatcttcg gcagccagct gtgcacgaat caggtcccgt
tcgcgctggc 2100aatcatgtac gtgatggacc tggtactgtt cttcctggac
acgtacctgt ggtacatcat 2160ctggctggtg atcttctcga tggtgcgcgc
gttcaagctt ggtatctcga tctggacgcc 2220ctggagcgag atcttcaccc
gcatgccgaa gcgtatttac gcaaagctgc tggcgacggc 2280cgagatggag
gtcaagtata agcccaaggt atgctgaatt caatctggtc aggtgaattc
2340accctcatat tgtggtacag gtgctcgtct cacaaatctg gaacgcggtc
atcatctcca 2400tgtaccggga gcatctcttg tccatcgagc acgtccagcg
cttgctttac caccaggttg 2460atggtcccga tggccgccgc accctcaggg
caccgccgtt cttcaccagc cagcgaactg 2520cgaagccagg cctgttcttc
cctcctggtg gcgaggctga gcgccgcatc tcgttctttg 2580cctcatcgct
gacgaccgcg ctcccggagc ctctgccgat cgacgccatg cccaccttca
2640ccgtgctcgt tccccattac tccgagaaga ttctgctcag tctgcgcgag
attatccgcg 2700aggaggacca gaacacccgc gttaccttac tggagtacct
caagcagctc caccctgtcg 2760aatgggacaa tttcgtcaag gacaccaaga
tcttggcgga agagtcggga gacgtccagg 2820acgagaagcg cgcgcgcacg
gacgacttgc cgttctattg catcgggttc aagacctcgt 2880caccagagta
caccctgcgt acgcgtatct gggcctcact gcgcgcacag acgctgtacc
2940gcacggtctc cggtatgatg aactactcca aggcgattaa gctcctctat
cgcgtcgaga 3000acccggatgt cgttcatgcc ttcggtggga acacggaacg
tcttgaacgc gagcttgagc 3060gcatgtctcg ccgcaagttc aagttcgtca
tctcgatgca gcggtactcc aagttcaaca 3120aggaggagca ggagaacgcc
gagttccttc tgcgcgcgta cccggatttg cagatcgcgt 3180acctcgatga
agagcccggt cccagcaaga gcgacgaggt tcggttgttt tcgacactca
3240tcgacggaca ctccgaggtg gacgagaaga cgggccgccg caagcccaag
ttccgcatcg 3300agctgcccgg taaccccatc ctcggtgacg ggaagtcgga
taaccagaac cacgccatcg 3360tcttctaccg cggcgagtac attcaggtca
ttgacgctaa ccaggacaat tacctggaag 3420agtgtctcaa gatccgtaat
gtcctgggcg agtttgagga atactccgtg tcgagccaga 3480gcccgtacgc
gcagtggggc cacaaggagt tcaacaagtg ccccgtcgct atcctgggtt
3540cccgcgagta catcttctcg gagaacatcg gtatcctcgg tgacatcgct
gccggcaagg 3600aacagacgtt cggtaccatt acggcgcgtg cgcttgcgtg
gatcggcggc aagctgcatt 3660acggtcaccc ggatttcctc aatgcgacgt
tcatgacgac gcgtggtggc gtgtcaaaag 3720cgcagaaggg cttgcatctt
aacgaggata tcttcgctgg tatgaccgcc gtgtcccgcg 3780gagggcgcat
caagcacatg gagtactacc agtgcggcaa aggtcgtgat ctcggattcg
3840gcacgatctt gaacttccag accaagatcg gtactggtat gggcgagcag
ctgctctcgc 3900gcgagtacta ctatctgggc acgcaattgc ctatcgaccg
gttcttgacg ttctactacg 3960cgcacgctgg tttccatgtc aacaacatcc
tggtcatcta ctccatccag gtcttcatgg 4020tcacccgtaa gtgcaggccc
tcatgaccgc cgagcaagca gtctaacgga tgtgcagtgc 4080tgtacctggg
cacattgaac aagcagctgt tcatctgcaa ggtcaactcc aatggccagg
4140ttcttagtgg acaagctggg tgctacaacc tcatcccggt cttcgagtgg
attcgccgga 4200gtatcatctc catcttcttg gtgttcttca tcgccttctt
gccgttgttc ttgcaaggta 4260tgttcacttc tcatgtgcca tttgtcaatc
gctcactcgt acgacagagc tttgcgaacg 4320cggaacagga aaggcgttgc
tgcgtctcgg gaagcacttc ctgtcactgt cgcccatctt 4380cgaagtgttc
tccacccaaa tctactcgca ggcgctcttg aacaacatga gtttcggtgg
4440tgcgcgctac atcgctacag gacgcggttt cgcgacgagt cggataccct
tcaacatcct 4500ctactcgcgt ttcgcgccgc cgagcatcta catgggcatg
cgtaatctgc tgctcttgct 4560gtacgcgacg atggccattt ggatcccaca
cctgatctac ttctggttct ccgtcctctc 4620cctctgcatc gcgccattca
tgttcaatcc gcatcaattc tcgtacgctg acttcatcat 4680cgactaccgg
gagttcttgc gctggatgtc gcgcggtaac tcgcggacga aggcgagtag
4740ctggtacgga tattgccgtc tgtcgcgtac cgcgattact gggtacaaga
agaagaaact 4800gggacacccg tcggagaagc tgtcgggcga tgtgccgcgt
gcgccgtgga ggaacgtcat 4860cttctcggag atcctttggc ccatcggcgc
gtgcatcatc ttcatcgtcg cgtacatgtt 4920cgtcaaatcg ttccctgacg
agcagggcaa cgcgccgccg agcccgctgg tccgcattct 4980gctcatcgcg
gttggcccta ctgtgtggaa cgcggcggtg ctcatcacgc tgttcttcct
5040gtcgctcttc ctgggcccga tgatggatgg ctgggtcaag ttcggctcag
tcatggcggc 5100acttgcgcat ggtctagcgc tcataggcat gctcacgttc
ttcgagttct tcgtacgtcc 5160ttcgcgttgt tgtggtcgag tgctttgcta
acaccgcctt cagtggttcc tcgagctctg 5220ggatgcctcg cacgccgtgc
tcggcgtcat cgccattatt gccgttcagc gcgggatcca 5280gaagatcctc
attgccgtct tcctgacgcg tgagtacaag cacgacgaga cgaaccgcgc
5340gtggtggaca ggtaaatggt atggacgcgg gctgggtacc tcggccatgt
cccagccggc 5400gcgcgagttc atcgtgaaga tcgtggagat gtcgctgtgg
acgtcggact tcctgcttgc 5460gcacctgttg ctcatcatct tgacggtgcc
gctactgctg ccgttcttca actcgatcca 5520ttcgacgatg ctttgtgagt
gatttgtagt cgttggtcac ggatgattgc tgactcgcgt 5580gcagtctggt
tgcgcccttc gaagcagatt aggcaacctc tgttctccac taagcagaag
5640cggcaacggc gatggattgt aagttccttt gattgctctg gctaccgacc
ttcgctcacc 5700tgtctcaggt catgaagtat accgtggtat atctcgtggt
ggtggctttc ctcgttgcgc 5760tcatcgctct gcgtacgttt tctgtcgcgc
tcaccctcta ttttcactaa cgtttcctcc 5820agccgcgctc ttccgcgaga
gcatccactt caactgcgag atctgccaga gtatatagtc 5880atataacgac
gtctatcgta tcgccggacg agagccccgt cgcctacaca ctgacatgga
5940attgctgtgt atacaatcga tcttctgacc gcgtcggggg cgttgccgtc
tttctactat 6000caacttgctt gtgtatcaac atttcttctc tccaagccta
cattgacata gagtaatagc 6060ccatgttcat acaacaatcg catagcattg
catataccat 610021740PRTSchizophyllum commune 2Met Ser Gly Pro Gly
Tyr Gly Arg Asn Pro Phe Asp Asn Pro Pro Pro 1 5 10 15 Asn Arg Gly
Pro Tyr Gly Gln Gln Pro Gly Phe Pro Gly Pro Gly Pro 20 25 30 Arg
Pro Tyr Asp Ser Asp Ala Asp Met Ser Gln Thr Tyr Gly Ser Thr 35 40
45 Thr Arg Leu Ala Gly Ser Ala Gly Tyr Ser Asp Arg Asn Gly Ser Phe
50 55 60 Asp Gly Asp Arg Ser Tyr Ala Pro Ser Ile Asp Ser Arg Ala
Ser Val 65 70 75 80 Pro Ser Ile Ser Pro Phe Ala Asp Pro Gly Ile Gly
Ser Asn Glu Pro 85 90 95 Tyr Pro Ala Trp Ser Val Glu Arg Gln Ile
Pro Met Ser Thr Glu Glu 100 105 110 Ile Glu Asp Ile Phe Leu Asp Leu
Thr Gln Lys Phe Gly Phe Gln Arg 115 120 125 Asp Ser Met Arg Asn Thr
Phe Asp Phe Met Met His Leu Leu Asp Ser 130 135 140 Arg Ala Ser Arg
Met Thr Pro Asn Gln Ala Leu Leu Thr Leu His Ala 145 150 155 160 Asp
Tyr Ile Gly Gly Gln His Ala Asn Tyr Arg Lys Trp Tyr Phe Ala 165 170
175 Ala Gln Leu Asn Leu Asp Asp Ala Val Gly Gln Thr Asn Asn Pro Gly
180 185 190 Ile Gln Arg Leu Lys Thr Ile Lys Gly Ala Thr Lys Thr Lys
Ser Leu 195 200 205 Asp Ser Ala Leu Asn Arg Trp Arg Asn Ala Met Asn
Asn Met Ser Gln 210 215 220 Tyr Asp Arg Leu Arg Gln Ile Ala Leu Tyr
Leu Leu Cys Trp Gly Glu 225 230 235 240 Ala Gly Asn Ile Arg Leu Ala
Pro Glu Cys Leu Cys Phe Ile Phe Lys 245 250 255 Cys Ala Asp Asp Tyr
Tyr Arg Ser Pro Glu Cys Gln Asn Arg Met Asp 260 265 270 Pro Val Pro
Glu Gly Leu Tyr Leu Gln Thr Val Ile Lys Pro Leu Tyr 275 280 285 Arg
Phe Leu Arg Asp Gln Ala Tyr Glu Val Val Asp Gly Lys Gln Val 290 295
300 Lys Arg Glu Lys Asp His Asp Gln Ile Ile Gly Tyr Asp Asp Val Asn
305 310 315 320 Gln Leu Phe Trp Tyr Pro Glu Gly Leu Ala Lys Ile Val
Met Ser Asp 325 330 335 Asn Thr Arg Leu Val Asp Val Pro Pro Ala Gln
Arg Phe Met Lys Phe 340 345 350 Ala Lys Ile Glu Trp Asn Arg Val Phe
Phe Lys Thr Tyr Phe Glu Lys 355 360 365 Arg Ser Thr Ala His Leu Leu
Val Asn Phe Asn Arg Ile Trp Ile Leu 370 375 380 His Val Ser Met Tyr
Phe Phe Tyr Thr Ala Phe Asn Ser Pro Arg Val 385 390 395 400 Tyr Ala
Pro His Gly Lys Leu Asp Pro Ser Pro Glu Met Thr Trp Ser 405 410 415
Ala Thr Ala Leu Gly Gly Ala Val Ser Thr Met Ile Met Ile Leu Ala 420
425 430 Thr Ile Ala Glu Tyr Thr Tyr Ile Pro Thr Thr Trp Asn Asn Ala
Ser 435 440 445 His Leu Thr Thr Arg Leu Ile Phe Leu Leu Val Ile Leu
Ala Leu Thr 450 455 460 Ala Gly Pro Thr Phe Tyr Ile Ala Met Ile Asp
Gly Arg Thr Asp Ile 465 470 475 480 Gly Gln Val Pro Leu Ile Val Ala
Ile Val Gln Phe Phe Ile Ser Val 485 490 495 Val Ala Thr Leu Ala Phe
Ala Thr Ile Pro Ser Gly Arg Met Phe Gly 500 505 510 Asp Arg Val Ala
Gly Lys Ser Arg Lys His Met Ala Ser Gln Thr Phe 515 520 525 Thr Ala
Ser Tyr Pro Ser Met Lys Arg Ser Ser Arg Val Ala Ser Ile 530 535 540
Met Leu Trp Leu Leu Val Phe Gly Cys Lys Tyr Val Glu Ser Tyr Phe 545
550 555 560 Phe Leu Thr Ser Ser Phe Ser Ser Pro Ile Ala Val Met Ala
Arg Thr 565 570 575 Lys Val Gln Gly Cys Asn Asp Arg Ile Phe Gly Ser
Gln Leu Cys Thr 580 585 590 Asn Gln Val Pro Phe Ala Leu Ala Ile Met
Tyr Val Met Asp Leu Val 595 600 605 Leu Phe Phe Leu Asp Thr Tyr Leu
Trp Tyr Ile Ile Trp Leu Val Ile 610 615 620 Phe Ser Met Val Arg Ala
Phe Lys Leu Gly Ile Ser Ile Trp Thr Pro 625 630 635 640 Trp Ser Glu
Ile Phe Thr Arg Met Pro Lys Arg Ile Tyr Ala Lys Leu 645 650 655 Leu
Ala Thr Ala Glu Met Glu Val Lys Tyr Lys Pro Lys Val Leu Val 660 665
670 Ser Gln Ile Trp Asn Ala Val Ile Ile Ser Met Tyr Arg Glu His Leu
675 680 685 Leu Ser Ile Glu His Val Gln Arg Leu Leu Tyr His Gln Val
Asp Gly 690 695 700 Pro Asp Gly Arg Arg Thr Leu Arg Ala Pro Pro Phe
Phe Thr Ser Gln 705 710 715 720 Arg Thr Ala Lys Pro Gly Leu Phe Phe
Pro Pro Gly Gly Glu Ala Glu 725 730 735 Arg Arg Ile Ser Phe Phe Ala
Ser Ser Leu Thr Thr Ala Leu Pro Glu 740 745 750 Pro Leu Pro Ile Asp
Ala Met Pro Thr Phe Thr Val Leu Val Pro His 755 760 765 Tyr Ser Glu
Lys Ile Leu Leu Ser Leu Arg Glu Ile Ile Arg Glu Glu 770 775 780 Asp
Gln Asn Thr Arg Val Thr Leu Leu Glu Tyr Leu Lys Gln Leu His 785 790
795 800 Pro Val Glu Trp Asp Asn Phe Val Lys Asp Thr Lys Ile Leu Ala
Glu 805 810 815 Glu Ser Gly Asp Val Gln Asp Glu Lys Arg Ala Arg Thr
Asp Asp Leu 820 825 830 Pro Phe Tyr Cys Ile Gly Phe Lys Thr Ser Ser
Pro Glu Tyr Thr Leu 835 840 845 Arg Thr Arg Ile Trp Ala Ser Leu Arg
Ala Gln Thr Leu Tyr Arg Thr 850 855 860 Val Ser Gly Met Met Asn Tyr
Ser Lys Ala Ile Lys Leu Leu Tyr Arg 865 870 875 880 Val Glu Asn Pro
Asp Val Val His Ala Phe Gly Gly Asn Thr Glu Arg 885 890 895 Leu Glu
Arg Glu Leu Glu Arg Met Ser Arg Arg Lys Phe Lys Phe Val 900 905 910
Ile Ser Met Gln Arg Tyr Ser Lys Phe Asn Lys Glu Glu Gln Glu Asn 915
920 925 Ala Glu Phe Leu Leu Arg Ala Tyr Pro Asp Leu Gln Ile Ala Tyr
Leu 930 935 940 Asp Glu Glu Pro Gly Pro Ser Lys Ser Asp Glu Val Arg
Leu Phe Ser 945 950 955 960 Thr Leu Ile Asp Gly His Ser Glu Val Asp
Glu Lys Thr Gly Arg Arg 965 970 975 Lys Pro Lys Phe Arg Ile Glu Leu
Pro Gly Asn Pro Ile Leu Gly Asp 980 985 990 Gly Lys Ser Asp Asn Gln
Asn His Ala Ile Val Phe Tyr Arg Gly Glu 995 1000 1005 Tyr Ile Gln
Val Ile Asp Ala Asn Gln Asp Asn Tyr Leu Glu Glu 1010 1015 1020 Cys
Leu Lys Ile Arg Asn Val Leu Gly Glu Phe Glu Glu Tyr Ser 1025 1030
1035 Val Ser Ser Gln Ser Pro Tyr Ala Gln Trp Gly His Lys Glu Phe
1040 1045 1050 Asn Lys Cys Pro Val Ala Ile Leu Gly Ser Arg Glu Tyr
Ile Phe 1055 1060 1065 Ser Glu Asn Ile Gly Ile Leu Gly Asp Ile Ala
Ala Gly Lys Glu 1070 1075 1080 Gln Thr Phe Gly Thr Ile Thr Ala Arg
Ala Leu Ala Trp Ile Gly 1085 1090 1095 Gly Lys Leu His Tyr Gly His
Pro Asp Phe Leu Asn Ala Thr Phe 1100 1105 1110 Met Thr Thr Arg Gly
Gly Val Ser Lys Ala Gln Lys Gly Leu His 1115 1120 1125 Leu Asn Glu
Asp Ile Phe Ala Gly Met Thr Ala Val Ser Arg Gly 1130 1135 1140 Gly
Arg Ile Lys His Met Glu Tyr Tyr Gln Cys Gly Lys Gly Arg 1145 1150
1155 Asp Leu Gly Phe Gly Thr Ile Leu Asn Phe Gln Thr Lys Ile Gly
1160 1165 1170 Thr Gly Met Gly Glu Gln Leu Leu Ser Arg Glu Tyr Tyr
Tyr Leu 1175 1180 1185 Gly Thr Gln Leu Pro Ile Asp Arg Phe Leu Thr
Phe Tyr Tyr Ala 1190 1195 1200 His Ala Gly Phe His Val Asn Asn Ile
Leu Val Ile Tyr Ser Ile 1205 1210 1215 Gln Val Phe Met Val Thr Leu
Leu Tyr Leu Gly Thr Leu Asn Lys 1220 1225 1230 Gln Leu Phe Ile Cys
Lys Val Asn Ser Asn Gly Gln Val Leu Ser 1235 1240 1245 Gly Gln Ala
Gly Cys Tyr Asn Leu Ile Pro Val Phe Glu Trp Ile 1250 1255 1260 Arg
Arg Ser Ile Ile Ser Ile Phe Leu Val Phe Phe Ile Ala Phe 1265 1270
1275 Leu Pro Leu Phe Leu Gln Glu Leu Cys Glu Arg Gly Thr Gly Lys
1280 1285 1290 Ala Leu Leu Arg Leu Gly Lys His Phe Leu Ser Leu Ser
Pro Ile 1295 1300 1305 Phe Glu Val Phe Ser Thr Gln Ile Tyr Ser Gln
Ala Leu Leu Asn 1310 1315 1320 Asn Met Ser Phe Gly Gly Ala Arg Tyr
Ile Ala Thr Gly Arg Gly 1325 1330 1335 Phe Ala Thr Ser Arg Ile Pro
Phe Asn Ile
Leu Tyr Ser Arg Phe 1340 1345 1350 Ala Pro Pro Ser Ile Tyr Met Gly
Met Arg Asn Leu Leu Leu Leu 1355 1360 1365 Leu Tyr Ala Thr Met Ala
Ile Trp Ile Pro His Leu Ile Tyr Phe 1370 1375 1380 Trp Phe Ser Val
Leu Ser Leu Cys Ile Ala Pro Phe Met Phe Asn 1385 1390 1395 Pro His
Gln Phe Ser Tyr Ala Asp Phe Ile Ile Asp Tyr Arg Glu 1400 1405 1410
Phe Leu Arg Trp Met Ser Arg Gly Asn Ser Arg Thr Lys Ala Ser 1415
1420 1425 Ser Trp Tyr Gly Tyr Cys Arg Leu Ser Arg Thr Ala Ile Thr
Gly 1430 1435 1440 Tyr Lys Lys Lys Lys Leu Gly His Pro Ser Glu Lys
Leu Ser Gly 1445 1450 1455 Asp Val Pro Arg Ala Pro Trp Arg Asn Val
Ile Phe Ser Glu Ile 1460 1465 1470 Leu Trp Pro Ile Gly Ala Cys Ile
Ile Phe Ile Val Ala Tyr Met 1475 1480 1485 Phe Val Lys Ser Phe Pro
Asp Glu Gln Gly Asn Ala Pro Pro Ser 1490 1495 1500 Pro Leu Val Arg
Ile Leu Leu Ile Ala Val Gly Pro Thr Val Trp 1505 1510 1515 Asn Ala
Ala Val Leu Ile Thr Leu Phe Phe Leu Ser Leu Phe Leu 1520 1525 1530
Gly Pro Met Met Asp Gly Trp Val Lys Phe Gly Ser Val Met Ala 1535
1540 1545 Ala Leu Ala His Gly Leu Ala Leu Ile Gly Met Leu Thr Phe
Phe 1550 1555 1560 Glu Phe Phe Trp Phe Leu Glu Leu Trp Asp Ala Ser
His Ala Val 1565 1570 1575 Leu Gly Val Ile Ala Ile Ile Ala Val Gln
Arg Gly Ile Gln Lys 1580 1585 1590 Ile Leu Ile Ala Val Phe Leu Thr
Arg Glu Tyr Lys His Asp Glu 1595 1600 1605 Thr Asn Arg Ala Trp Trp
Thr Gly Lys Trp Tyr Gly Arg Gly Leu 1610 1615 1620 Gly Thr Ser Ala
Met Ser Gln Pro Ala Arg Glu Phe Ile Val Lys 1625 1630 1635 Ile Val
Glu Met Ser Leu Trp Thr Ser Asp Phe Leu Leu Ala His 1640 1645 1650
Leu Leu Leu Ile Ile Leu Thr Val Pro Leu Leu Leu Pro Phe Phe 1655
1660 1665 Asn Ser Ile His Ser Thr Met Leu Phe Trp Leu Arg Pro Ser
Lys 1670 1675 1680 Gln Ile Arg Gln Pro Leu Phe Ser Thr Lys Gln Lys
Arg Gln Arg 1685 1690 1695 Arg Trp Ile Val Met Lys Tyr Thr Val Val
Tyr Leu Val Val Val 1700 1705 1710 Ala Phe Leu Val Ala Leu Ile Ala
Leu Pro Ala Leu Phe Arg Glu 1715 1720 1725 Ser Ile His Phe Asn Cys
Glu Ile Cys Gln Ser Ile 1730 1735 1740 3 5770DNASchizophyllum
commune 3ctgtccaaag aagagatcga ggacatcttc ctcgatctga cgcagaagtt
tggctttcag 60cgggattcca tgcggaacat ggtacgtggc gtatgcccat gtgcggcgtt
ctgaggccta 120aacgttttcc gccagttcga cttcaccatg cagctgcttg
acagccgagc gtctcgtatg 180acccccaacc aggcgctcct caccctccac
gccgactaca ttggtggcca gcatgcgaac 240taccggaagt ggtacttcgc
ggcgcagctc gaccttgacg acgccgtggg acaaactcag 300aatccgggtc
tcaaccgcct caagtccact cgcggatcgg gcaagcgacc acgccatgaa
360aagtcgctga acacggcatt ggagcgctgg cggcaagcca tgaacaacat
gtcgcagtat 420gaccgcttac gccagatcgc gctctacctg ctctgctggg
gcgaagcggc gcaagtgcga 480ttcatgcccg agtgcttgtg cttcatcttc
aagtgcgccg acgactatta tcgttcgccg 540gagtgccaga acaggatgga
gccggtaccg gagggtctct acctgaggac ggtcgtaaag 600ccgctctaca
gatttgtccg ggatcaaggc tatgaggtgg tggagggaaa attcgtacgg
660cgggaacggg atcacgacca aatcattggt tacgatgacg tgaatcagct
gttctggtac 720ccggagggca ttgcccgtat cgtcctgtcg gacaaggtaa
gcacctctgt gcatcttctg 780tgacatacag ggctaattgt cgagcagagt
cgtctggtcg acctccctcc agcacagcgc 840ttcatgaagt tcgaccgtat
cgagtggaat cgcgtcttct tcaagacgtt ctacgagact 900cgatccttta
cgcatctttt ggtcgacttc aaccgtatct gggtcgtgca catcgctctc
960tacttcttct acaccgcata caactccccc acgatctacg ccatcaacgg
caacactccg 1020acgtctctgg cttggagcgc gactgcgctc ggcggtgcgg
tagcgacagg tatcatgatc 1080ctcgccacga tcgccgagtt ctcgcacatc
cccacgacat ggaacaacac ctcgcatctg 1140actcgccgcc tcgccttcct
cctcgtcacg ctcggcctca catgtggtcc gacgttctac 1200gtcgcgattg
cagagagcaa cgggagcggc ggctctttgg ccttgattct cggcatcgtc
1260cagttcttca tctccgtcgt agcgactgcg ctcttcacta tcatgccttc
tggtcgtatg 1320ttcggcgacc gcgtcgcagg caagagtcgc aagtatctcg
ccagccagac gttcacggcc 1380agctacccgt cgttgcccaa gcaccagcgg
ttcgcatcac tcctgatgtg gttcctcatc 1440ttcgggtgca agttgacgga
gagttacttc ttcctgacgt tgtccttccg cgaccctatt 1500cgcgtcatgg
tcggcatgaa gatccagaac tgcgaggaca agattttcgg cagcggcctt
1560tgcaggaatc acgcagcatt caccctcacg atcatgtaca tcatggacct
cgtcttgttc 1620ttcctcgaca ccttcctttg gtatgtcatc tggaactcgg
ttttcagtat cgcacgctct 1680ttcgtactcg gcctttcgat ctggacacca
tggagggaca tcttccagcg tctgccgaag 1740cgtatctacg cgaagcttct
agcgaccggc gacatggagg tcaagtacaa gcccaaggtg 1800tgtgaatagc
tcgctgtaag gttcttgatt ctgactcatt cgcaggtctt ggtttcgcaa
1860atctggaacg ccatcatcat ctccatgtac cgcgagcact tgctctctat
cgagcacgtt 1920caaaagctcc tgtaccatca agtggacact ggcgaagccg
gcaagcggag tcttcgcgcg 1980cctccgttct tcgtcgcgca gggcagcagc
ggtggctcgg gcgagttctt cccgcctggt 2040agcgaggctg agcgtcgtat
ctctttcttc gcgcagtctc tatctacgga gattcctcag 2100cccatcccgg
ttgacgccat gccgacgttc acagtgctta cgcctcacta cagcgagaag
2160gtgcgctttt tcctgggcgc attcaacatt agctgactgt cgtgcacaga
tccttctttc 2220gctccgtgag attatccgcg aggaggacca gaacacccgc
gtgacattgc ttgagtatct 2280caagcagctt cacccggtcg agtgggagaa
cttcgtcaag gacaccaaga ttttggccga 2340ggagtccgct atgttcaacg
gtccaagtcc tttcggcaac gatgagaagg gtcagtccaa 2400gatggacgat
cttcctttct actgcatcgg tttcaagagc gccgcgcccg agtacaccct
2460ccgcacccgt atctgggcgt ccttgcgcgc gcagaccctc taccgcacgg
tctccggcat 2520gatgaactat gcgaaggcga ttaagctgct ctaccgcgtc
gagaaccccg aggtcgtgca 2580gcagttcggc ggtaacacgg acaagctcga
gcgcgagttg gagcggatgg cccggcggaa 2640gttcaagttc ctggtgtcca
tgcagcgcta ctcgaagttc aacaaggagg agcacgagaa 2700cgccgagttc
ttgctccgcg cgtacccgga cctgcagatc gcgtacctgg aggaagagcc
2760tcctcgcaag gagggtggcg atccacgcat cttctctgcc ctcgtcgacg
gccacagcga 2820catcatcccg gagaccggca agcggcgccc caagttccgc
atcgagctgc ccggcaaccc 2880cattctcggt gacggcaagt cggacaacca
gaaccacgcc atcgtcttct accgcggcga 2940gtacctccag cttatcgacg
ccaaccagga caactacctc gaggagtgct tgaagatccg 3000taacgtactc
gccgagttcg aggagtacga cgtctctagc cagagtccgt acgcgcagtg
3060gagtgtcaag gagttcaagc gctccccggt cgccatcgtc ggtgcacgcg
agtatatctt 3120ctcggagcac atcggtattc tcggtgattt ggcggctggc
aaggaacaga cgttcggtac 3180gctcacggca cgcaacaacg ccttccttgg
cggcaagctg cactacggtc acccggattt 3240cctcaacgcc ctctacatga
acacgcgcgg tggtgtctcc aaggcgcaga agggtctcca 3300tctcaacgag
gatatttacg ccggtatgaa cgcggtcggt cgcggtggac gcatcaagca
3360tagcgaatac taccagtgcg gcaagggtcg tgacctcggt tttggcacca
tcttgaactt 3420ccagaccaag atcggtacgg gtatgggcga gcagatcctc
tcgcgcgagt actactacct 3480cggaacccaa ttgcccatcg atcgcttcct
cacgttctac tacgcgcacc caggtttcca 3540gatcaacaac atgctggtta
tcctatccgt gcaggtcttc atcgttacca gtacgttgat 3600tgcatatcgt
tagcctgaca gcgtctgacg aattcccagt ggtcttcctc ggtaccttga
3660agtcttcggt cacgatctgc aagtacacgt ccagcggtca gtacatcggt
ggtcaatccg 3720gttgctacaa cctcgtcccg gtcttccagt ggatcgagcg
ctgcatcatc agcatcttct 3780tggtgttcat gatcgctttc atgccgctct
tcctgcaagg taagagctcg tcaacctgct 3840caagggcctt gcgctgatca
tcatctcaga actcgtcgag cgcggtacct ggagtgccat 3900ctggcgtctg
ctcaagcagt ttatgtcgct gtcgcctgtc ttcgaggtgt tctccaccca
3960gattcagaca cactccgtgt tgagcaactt gacgttcggt ggtgcgcgtt
acatcgctac 4020cggtcgtggg ttcgccacca gtcgtatcag cttcagcatc
ttgttctcgc gtttcgcagg 4080cccgagtatc tacctcggca tgcgcacgct
cattatgctg ctctacgtga cgttgacgat 4140ctggacgcca tgggtcattt
acttctgggt ttccattctc tcgctctgca tcgcgccgtt 4200cttgttcaat
ccgcatcaat tcgtcttctc ggatttcctc atcgactaca ggtacgtcgg
4260acgagcgctg ttccgcgacg taagctgacc ggttatacag ggaatacctc
cggtggatgt 4320cgcgtggtaa ctcgcgctcg cacaacaact cctggattgg
gtactgccgg ttgtcccgca 4380cgatgatcac tgggtacaag aagaagaagc
tgggccaccc gtcggagaag ctttccggcg 4440acgttcctcg tgcaggctgg
cgcgccgtct tattctcgga gatcatcttc ccggcatgca 4500tggccatcct
cttcatcatc gcgtacatgt tcgtcaagtc gttccctctc gacggcaagc
4560agcctccctc cggcctcgtt cgcatcgccg tcgtgtctat cggccccatc
gtgtggaacg 4620ccgccatcct gttgacgctc ttccttgtgt cgttgttcct
cggccccatg ctcgacccgg 4680tcttccccct cttcggttcc gttatggcct
tcatcgcgca tttcctcggc acaatcggaa 4740tgattgggtt cttcgagttc
ctggtatgtg cccatacctt tcattcgtct tcaactatct 4800aacagattca
tagtggttcc tcgagtcctg ggaggcgtcg catgccgtgc tgggtctcat
4860cgccgtcatc tccatccagc gcgccattca caaaattctt atcgccgttt
tcctcagtcg 4920cgagttcaag cacgacgaga cgaacagggc ttggtggact
ggtcgctggt atggccgtgg 4980cctcggcacg cacgccatgt cgcagccggc
gcgtgagttc gtcgtcaaga tcatcgagtt 5040gtcgctctgg agctcggatc
tcatactcgg ccacatcctg ctgttcatgc ttactccggc 5100tgtcctcatc
ccgtacttcg accgtctgca cgccatgatg ctctgtacgt cgtgtctcat
5160tgtttgtgtt ggtcatactc ttaccctctc ttagtctggc tgcgcccctc
aaagcaaatc 5220cgcgcgcctc tgtactcaat caagcagaag aggcaaagac
gctggattgt cagtgttcag 5280tgccttattc tatcagctct tactgacgtc
ttcatagatc atgaagtacg gtactgtata 5340cgttaccgtc atcgcgatct
tcgtcgcgct catcgcgctt cgtgagtacc cttgctatct 5400ttcgtacctg
agcgtcgctg acccctttcc cagccctcgt cttccgacac actctaaagg
5460tcgagtgctc cctttgcgac agcttgtaat atcggactcg tatatatcta
gacttctccg 5520caccatgtgt agctgacgct tgggtatact tcgcggtgcc
gagctaattg tcgacggaca 5580ttctccatcg ttgagtgcag cgacatcggg
tggtttacga cacggacact tttcattgta 5640ccctctacga atgcaagaac
tctcttacga ccagtaccta tgtgctaagc cgtcgcctgt 5700tcaggatcat
acatacatac gtttctagat accttacagt taggcctatt cagggagagt
5760ctgcataaaa 577041622PRTSchizophyllum commune 4Met Arg Asn Met
Phe Asp Phe Thr Met Gln Leu Leu Asp Ser Arg Ala 1 5 10 15 Ser Arg
Met Thr Pro Asn Gln Ala Leu Leu Thr Leu His Ala Asp Tyr 20 25 30
Ile Gly Gly Gln His Ala Asn Tyr Arg Lys Trp Tyr Phe Ala Ala Gln 35
40 45 Leu Asp Leu Asp Asp Ala Val Gly Gln Thr Gln Asn Pro Gly Leu
Asn 50 55 60 Arg Leu Lys Ser Thr Arg Gly Ser Gly Lys Arg Pro Arg
His Glu Lys 65 70 75 80 Ser Leu Asn Thr Ala Leu Glu Arg Trp Arg Gln
Ala Met Asn Asn Met 85 90 95 Ser Gln Tyr Asp Arg Leu Arg Gln Ile
Ala Leu Tyr Leu Leu Cys Trp 100 105 110 Gly Glu Ala Ala Gln Val Arg
Phe Met Pro Glu Cys Leu Cys Phe Ile 115 120 125 Phe Lys Cys Ala Asp
Asp Tyr Tyr Arg Ser Pro Glu Cys Gln Asn Arg 130 135 140 Met Glu Pro
Val Pro Glu Gly Leu Tyr Leu Arg Thr Val Val Lys Pro 145 150 155 160
Leu Tyr Arg Phe Val Arg Asp Gln Gly Tyr Glu Val Val Glu Gly Lys 165
170 175 Phe Val Arg Arg Glu Arg Asp His Asp Gln Ile Ile Gly Tyr Asp
Asp 180 185 190 Val Asn Gln Leu Phe Trp Tyr Pro Glu Gly Ile Ala Arg
Ile Val Leu 195 200 205 Ser Asp Lys Ser Arg Leu Val Asp Leu Pro Pro
Ala Gln Arg Phe Met 210 215 220 Lys Phe Asp Arg Ile Glu Trp Asn Arg
Val Phe Phe Lys Thr Phe Tyr 225 230 235 240 Glu Thr Arg Ser Phe Thr
His Leu Leu Val Asp Phe Asn Arg Ile Trp 245 250 255 Val Val His Ile
Ala Leu Tyr Phe Phe Tyr Thr Ala Tyr Asn Ser Pro 260 265 270 Thr Ile
Tyr Ala Ile Asn Gly Asn Thr Pro Thr Ser Leu Ala Trp Ser 275 280 285
Ala Thr Ala Leu Gly Gly Ala Val Ala Thr Gly Ile Met Ile Leu Ala 290
295 300 Thr Ile Ala Glu Phe Ser His Ile Pro Thr Thr Trp Asn Asn Thr
Ser 305 310 315 320 His Leu Thr Arg Arg Leu Ala Phe Leu Leu Val Thr
Leu Gly Leu Thr 325 330 335 Cys Gly Pro Thr Phe Tyr Val Ala Ile Ala
Glu Ser Asn Gly Ser Gly 340 345 350 Gly Ser Leu Ala Leu Ile Leu Gly
Ile Val Gln Phe Phe Ile Ser Val 355 360 365 Val Ala Thr Ala Leu Phe
Thr Ile Met Pro Ser Gly Arg Met Phe Gly 370 375 380 Asp Arg Val Ala
Gly Lys Ser Arg Lys Tyr Leu Ala Ser Gln Thr Phe 385 390 395 400 Thr
Ala Ser Tyr Pro Ser Leu Pro Lys His Gln Arg Phe Ala Ser Leu 405 410
415 Leu Met Trp Phe Leu Ile Phe Gly Cys Lys Leu Thr Glu Ser Tyr Phe
420 425 430 Phe Leu Thr Leu Ser Phe Arg Asp Pro Ile Arg Val Met Val
Gly Met 435 440 445 Lys Ile Gln Asn Cys Glu Asp Lys Ile Phe Gly Ser
Gly Leu Cys Arg 450 455 460 Asn His Ala Ala Phe Thr Leu Thr Ile Met
Tyr Ile Met Asp Leu Val 465 470 475 480 Leu Phe Phe Leu Asp Thr Phe
Leu Trp Tyr Val Ile Trp Asn Ser Val 485 490 495 Phe Ser Ile Ala Arg
Ser Phe Val Leu Gly Leu Ser Ile Trp Thr Pro 500 505 510 Trp Arg Asp
Ile Phe Gln Arg Leu Pro Lys Arg Ile Tyr Ala Lys Leu 515 520 525 Leu
Ala Thr Gly Asp Met Glu Val Lys Tyr Lys Pro Lys Val Leu Val 530 535
540 Ser Gln Ile Trp Asn Ala Ile Ile Ile Ser Met Tyr Arg Glu His Leu
545 550 555 560 Leu Ser Ile Glu His Val Gln Lys Leu Leu Tyr His Gln
Val Asp Thr 565 570 575 Gly Glu Ala Gly Lys Arg Ser Leu Arg Ala Pro
Pro Phe Phe Val Ala 580 585 590 Gln Gly Ser Ser Gly Gly Ser Gly Glu
Phe Phe Pro Pro Gly Ser Glu 595 600 605 Ala Glu Arg Arg Ile Ser Phe
Phe Ala Gln Ser Leu Ser Thr Glu Ile 610 615 620 Pro Gln Pro Ile Pro
Val Asp Ala Met Pro Thr Phe Thr Val Leu Thr 625 630 635 640 Pro His
Tyr Ser Glu Lys Ile Leu Leu Ser Leu Arg Glu Ile Ile Arg 645 650 655
Glu Glu Asp Gln Asn Thr Arg Val Thr Leu Leu Glu Tyr Leu Lys Gln 660
665 670 Leu His Pro Val Glu Trp Glu Asn Phe Val Lys Asp Thr Lys Ile
Leu 675 680 685 Ala Glu Glu Ser Ala Met Phe Asn Gly Pro Ser Pro Phe
Gly Asn Asp 690 695 700 Glu Lys Gly Gln Ser Lys Met Asp Asp Leu Pro
Phe Tyr Cys Ile Gly 705 710 715 720 Phe Lys Ser Ala Ala Pro Glu Tyr
Thr Leu Arg Thr Arg Ile Trp Ala 725 730 735 Ser Leu Arg Ala Gln Thr
Leu Tyr Arg Thr Val Ser Gly Met Met Asn 740 745 750 Tyr Ala Lys Ala
Ile Lys Leu Leu Tyr Arg Val Glu Asn Pro Glu Val 755 760 765 Val Gln
Gln Phe Gly Gly Asn Thr Asp Lys Leu Glu Arg Glu Leu Glu 770 775 780
Arg Met Ala Arg Arg Lys Phe Lys Phe Leu Val Ser Met Gln Arg Tyr 785
790 795 800 Ser Lys Phe Asn Lys Glu Glu His Glu Asn Ala Glu Phe Leu
Leu Arg 805 810 815 Ala Tyr Pro Asp Leu Gln Ile Ala Tyr Leu Glu Glu
Glu Pro Pro Arg 820 825 830 Lys Glu Gly Gly Asp Pro Arg Ile Phe Ser
Ala Leu Val Asp Gly His 835 840 845 Ser Asp Ile Ile Pro Glu Thr Gly
Lys Arg Arg Pro Lys Phe Arg Ile 850 855 860 Glu Leu Pro Gly Asn Pro
Ile Leu Gly Asp Gly Lys Ser Asp Asn Gln 865 870 875 880 Asn His Ala
Ile Val Phe Tyr Arg Gly Glu Tyr Leu Gln Leu Ile Asp 885 890 895 Ala
Asn Gln Asp Asn Tyr Leu Glu Glu Cys Leu Lys Ile Arg Asn Val 900 905
910 Leu Ala Glu Phe Glu Glu Tyr Asp Val Ser Ser Gln Ser Pro Tyr Ala
915 920 925 Gln Trp Ser Val Lys Glu Phe Lys Arg Ser Pro Val Ala Ile
Val Gly 930 935 940 Ala Arg Glu Tyr Ile Phe Ser Glu His Ile Gly Ile
Leu Gly Asp Leu 945 950 955 960 Ala Ala Gly Lys Glu Gln Thr Phe Gly
Thr Leu Thr Ala Arg Asn Asn 965 970 975 Ala Phe Leu Gly Gly Lys Leu
His Tyr Gly His Pro Asp Phe Leu Asn 980
985 990 Ala Leu Tyr Met Asn Thr Arg Gly Gly Val Ser Lys Ala Gln Lys
Gly 995 1000 1005 Leu His Leu Asn Glu Asp Ile Tyr Ala Gly Met Asn
Ala Val Gly 1010 1015 1020 Arg Gly Gly Arg Ile Lys His Ser Glu Tyr
Tyr Gln Cys Gly Lys 1025 1030 1035 Gly Arg Asp Leu Gly Phe Gly Thr
Ile Leu Asn Phe Gln Thr Lys 1040 1045 1050 Ile Gly Thr Gly Met Gly
Glu Gln Ile Leu Ser Arg Glu Tyr Tyr 1055 1060 1065 Tyr Leu Gly Thr
Gln Leu Pro Ile Asp Arg Phe Leu Thr Phe Tyr 1070 1075 1080 Tyr Ala
His Pro Gly Phe Gln Ile Asn Asn Met Leu Val Ile Leu 1085 1090 1095
Ser Val Gln Val Phe Ile Val Thr Met Val Phe Leu Gly Thr Leu 1100
1105 1110 Lys Ser Ser Val Thr Ile Cys Lys Tyr Thr Ser Ser Gly Gln
Tyr 1115 1120 1125 Ile Gly Gly Gln Ser Gly Cys Tyr Asn Leu Val Pro
Val Phe Gln 1130 1135 1140 Trp Ile Glu Arg Cys Ile Ile Ser Ile Phe
Leu Val Phe Met Ile 1145 1150 1155 Ala Phe Met Pro Leu Phe Leu Gln
Glu Leu Val Glu Arg Gly Thr 1160 1165 1170 Trp Ser Ala Ile Trp Arg
Leu Leu Lys Gln Phe Met Ser Leu Ser 1175 1180 1185 Pro Val Phe Glu
Val Phe Ser Thr Gln Ile Gln Thr His Ser Val 1190 1195 1200 Leu Ser
Asn Leu Thr Phe Gly Gly Ala Arg Tyr Ile Ala Thr Gly 1205 1210 1215
Arg Gly Phe Ala Thr Ser Arg Ile Ser Phe Ser Ile Leu Phe Ser 1220
1225 1230 Arg Phe Ala Gly Pro Ser Ile Tyr Leu Gly Met Arg Thr Leu
Ile 1235 1240 1245 Met Leu Leu Tyr Val Thr Leu Thr Ile Trp Thr Pro
Trp Val Ile 1250 1255 1260 Tyr Phe Trp Val Ser Ile Leu Ser Leu Cys
Ile Ala Pro Phe Leu 1265 1270 1275 Phe Asn Pro His Gln Phe Val Phe
Ser Asp Phe Leu Ile Asp Tyr 1280 1285 1290 Arg Glu Tyr Leu Arg Trp
Met Ser Arg Gly Asn Ser Arg Ser His 1295 1300 1305 Asn Asn Ser Trp
Ile Gly Tyr Cys Arg Leu Ser Arg Thr Met Ile 1310 1315 1320 Thr Gly
Tyr Lys Lys Lys Lys Leu Gly His Pro Ser Glu Lys Leu 1325 1330 1335
Ser Gly Asp Val Pro Arg Ala Gly Trp Arg Ala Val Leu Phe Ser 1340
1345 1350 Glu Ile Ile Phe Pro Ala Cys Met Ala Ile Leu Phe Ile Ile
Ala 1355 1360 1365 Tyr Met Phe Val Lys Ser Phe Pro Leu Asp Gly Lys
Gln Pro Pro 1370 1375 1380 Ser Gly Leu Val Arg Ile Ala Val Val Ser
Ile Gly Pro Ile Val 1385 1390 1395 Trp Asn Ala Ala Ile Leu Leu Thr
Leu Phe Leu Val Ser Leu Phe 1400 1405 1410 Leu Gly Pro Met Leu Asp
Pro Val Phe Pro Leu Phe Gly Ser Val 1415 1420 1425 Met Ala Phe Ile
Ala His Phe Leu Gly Thr Ile Gly Met Ile Gly 1430 1435 1440 Phe Phe
Glu Phe Leu Trp Phe Leu Glu Ser Trp Glu Ala Ser His 1445 1450 1455
Ala Val Leu Gly Leu Ile Ala Val Ile Ser Ile Gln Arg Ala Ile 1460
1465 1470 His Lys Ile Leu Ile Ala Val Phe Leu Ser Arg Glu Phe Lys
His 1475 1480 1485 Asp Glu Thr Asn Arg Ala Trp Trp Thr Gly Arg Trp
Tyr Gly Arg 1490 1495 1500 Gly Leu Gly Thr His Ala Met Ser Gln Pro
Ala Arg Glu Phe Val 1505 1510 1515 Val Lys Ile Ile Glu Leu Ser Leu
Trp Ser Ser Asp Leu Ile Leu 1520 1525 1530 Gly His Ile Leu Leu Phe
Met Leu Thr Pro Ala Val Leu Ile Pro 1535 1540 1545 Tyr Phe Asp Arg
Leu His Ala Met Met Leu Phe Trp Leu Arg Pro 1550 1555 1560 Ser Lys
Gln Ile Arg Ala Pro Leu Tyr Ser Ile Lys Gln Lys Arg 1565 1570 1575
Gln Arg Arg Trp Ile Ile Met Lys Tyr Gly Thr Val Tyr Val Thr 1580
1585 1590 Val Ile Ala Ile Phe Val Ala Leu Ile Ala Leu Pro Leu Val
Phe 1595 1600 1605 Arg His Thr Leu Lys Val Glu Cys Ser Leu Cys Asp
Ser Leu 1610 1615 1620 5 5223DNASchizophyllum commune 5atgtccggcc
caggatatgg caggaatcca ttcgacaatc ccccgcccaa cagaggtccc 60tatggccagc
agccaggttt cccggggccc ggccctcggc cttacgactc ggacgcggac
120atgagccaga cctatggcag cacaaccagg ctcgccggca gtgccggtta
cagcgacaga 180aacggcagct tcgacggcga ccgctcctac gcgccctcaa
ttgactcgcg cgccagcgtg 240cccagcatat cgcccttcgc agacccgggt
atcggctcta atgagccgta tcccgcttgg 300tcggtcgaac gccagattcc
catgtccacg gaggagattg aggacatctt cctcgacctc 360acccaaaagt
ttggcttcca gcgcgactcc atgcggaata cgttcgactt catgatgcac
420ctcctcgatt cccgtgcctc gcgcatgacg cccaaccaag ctctgctcac
gcttcacgcc 480gactacattg gtggccagca tgccaattac cggaagtggt
atttcgccgc acagctcaac 540ctcgatgacg cggtcgggca aaccaataac
cccggtatcc agcgcttgaa gaccatcaag 600ggcgctacga agaccaagtc
gctcgacagc gcactcaacc gctggcgcaa cgcgatgaac 660aacatgagcc
agtacgatcg cctccggcaa attgcgctct acctcctctg ctggggtgaa
720gcaggcaaca tccgtctggc gcccgagtgc ttgtgcttca tcttcaagtg
cgcggacgac 780tactacagaa gtcccgagtg tcagaaccgg atggaccccg
tgccggaagg gctgtacctg 840cagacggtca tcaagccgct ctatcgcttc
ctacgtgatc aggcgtacga agtcgttgat 900gggaagcaag tgaagcgcga
gaaggaccac gaccagatta tcggttatga cgacgtcaac 960cagttattct
ggtatccgga aggtttggct aagatcgtca tgtcggacaa cacacgactt
1020gtagatgtac ctccggcgca gcggttcatg aagttcgcca agatcgagtg
gaaccgcgtc 1080ttcttcaaga cgtactttga gaagcgctct actgcccatc
tcctggtcaa cttcaaccgt 1140atatggatcc tccacgtctc gatgtacttc
ttctacacgg cattcaactc tccacgagtc 1200tacgcgccgc acggcaaact
cgacccctcc cctgagatga cctggtccgc gactgccctt 1260ggaggcgctg
tgtccaccat gatcatgatc cttgccacta tcgcggagta cacctacatc
1320cccacgacat ggaacaatgc gtcgcacctc accacgcggc tcattttcct
cctggtcatc 1380ctcgcgctca ctgctggccc aacattctat atcgccatga
tagacggacg cacggacatc 1440ggccaagtac cactcatcgt ggccatagtg
cagttcttca tctccgtcgt cgccaccctc 1500gctttcgcta ccatcccttc
tggtcgcatg ttcggcgacc gtgtggctgg caagtcaaga 1560aagcacatgg
catcgcagac gttcacagcg tcgtacccgt ccatgaagcg gtcatctcgc
1620gtagcgagta tcatgctgtg gcttttggtc tttggctgca aatacgtcga
gtcttacttc 1680ttcttgacgt cctccttctc cagcccgatc gcggtcatgg
cgcgtacgaa ggtacagggc 1740tgcaacgacc gtatcttcgg cagccagctg
tgcacgaatc aggtcccgtt cgcgctggca 1800atcatgtacg tgatggacct
ggtactgttc ttcctggaca cgtacctgtg gtacatcatc 1860tggctggtga
tcttctcgat ggtgcgcgcg ttcaagcttg gtatctcgat ctggacgccc
1920tggagcgaga tcttcacccg catgccgaag cgtatttacg caaagctgct
ggcgacggcc 1980gagatggagg tcaagtataa gcccaaggtg ctcgtctcac
aaatctggaa cgcggtcatc 2040atctccatgt accgggagca tctcttgtcc
atcgagcacg tccagcgctt gctttaccac 2100caggttgatg gtcccgatgg
ccgccgcacc ctcagggcac cgccgttctt caccagccag 2160cgaactgcga
agccaggcct gttcttccct cctggtggcg aggctgagcg ccgcatctcg
2220ttctttgcct catcgctgac gaccgcgctc ccggagcctc tgccgatcga
cgccatgccc 2280accttcaccg tgctcgttcc ccattactcc gagaagattc
tgctcagtct gcgcgagatt 2340atccgcgagg aggaccagaa cacccgcgtt
accttactgg agtacctcaa gcagctccac 2400cctgtcgaat gggacaattt
cgtcaaggac accaagatct tggcggaaga gtcgggagac 2460gtccaggacg
agaagcgcgc gcgcacggac gacttgccgt tctattgcat cgggttcaag
2520acctcgtcac cagagtacac cctgcgtacg cgtatctggg cctcactgcg
cgcacagacg 2580ctgtaccgca cggtctccgg tatgatgaac tactccaagg
cgattaagct cctctatcgc 2640gtcgagaacc cggatgtcgt tcatgccttc
ggtgggaaca cggaacgtct tgaacgcgag 2700cttgagcgca tgtctcgccg
caagttcaag ttcgtcatct cgatgcagcg gtactccaag 2760ttcaacaagg
aggagcagga gaacgccgag ttccttctgc gcgcgtaccc ggatttgcag
2820atcgcgtacc tcgatgaaga gcccggtccc agcaagagcg acgaggttcg
gttgttttcg 2880acactcatcg acggacactc cgaggtggac gagaagacgg
gccgccgcaa gcccaagttc 2940cgcatcgagc tgcccggtaa ccccatcctc
ggtgacggga agtcggataa ccagaaccac 3000gccatcgtct tctaccgcgg
cgagtacatt caggtcattg acgctaacca ggacaattac 3060ctggaagagt
gtctcaagat ccgtaatgtc ctgggcgagt ttgaggaata ctccgtgtcg
3120agccagagcc cgtacgcgca gtggggccac aaggagttca acaagtgccc
cgtcgctatc 3180ctgggttccc gcgagtacat cttctcggag aacatcggta
tcctcggtga catcgctgcc 3240ggcaaggaac agacgttcgg taccattacg
gcgcgtgcgc ttgcgtggat cggcggcaag 3300ctgcattacg gtcacccgga
tttcctcaat gcgacgttca tgacgacgcg tggtggcgtg 3360tcaaaagcgc
agaagggctt gcatcttaac gaggatatct tcgctggtat gaccgccgtg
3420tcccgcggag ggcgcatcaa gcacatggag tactaccagt gcggcaaagg
tcgtgatctc 3480ggattcggca cgatcttgaa cttccagacc aagatcggta
ctggtatggg cgagcagctg 3540ctctcgcgcg agtactacta tctgggcacg
caattgccta tcgaccggtt cttgacgttc 3600tactacgcgc acgctggttt
ccatgtcaac aacatcctgg tcatctactc catccaggtc 3660ttcatggtca
ccctgctgta cctgggcaca ttgaacaagc agctgttcat ctgcaaggtc
3720aactccaatg gccaggttct tagtggacaa gctgggtgct acaacctcat
cccggtcttc 3780gagtggattc gccggagtat catctccatc ttcttggtgt
tcttcatcgc cttcttgccg 3840ttgttcttgc aagagctttg cgaacgcgga
acaggaaagg cgttgctgcg tctcgggaag 3900cacttcctgt cactgtcgcc
catcttcgaa gtgttctcca cccaaatcta ctcgcaggcg 3960ctcttgaaca
acatgagttt cggtggtgcg cgctacatcg ctacaggacg cggtttcgcg
4020acgagtcgga tacccttcaa catcctctac tcgcgtttcg cgccgccgag
catctacatg 4080ggcatgcgta atctgctgct cttgctgtac gcgacgatgg
ccatttggat cccacacctg 4140atctacttct ggttctccgt cctctccctc
tgcatcgcgc cattcatgtt caatccgcat 4200caattctcgt acgctgactt
catcatcgac taccgggagt tcttgcgctg gatgtcgcgc 4260ggtaactcgc
ggacgaaggc gagtagctgg tacggatatt gccgtctgtc gcgtaccgcg
4320attactgggt acaagaagaa gaaactggga cacccgtcgg agaagctgtc
gggcgatgtg 4380ccgcgtgcgc cgtggaggaa cgtcatcttc tcggagatcc
tttggcccat cggcgcgtgc 4440atcatcttca tcgtcgcgta catgttcgtc
aaatcgttcc ctgacgagca gggcaacgcg 4500ccgccgagcc cgctggtccg
cattctgctc atcgcggttg gccctactgt gtggaacgcg 4560gcggtgctca
tcacgctgtt cttcctgtcg ctcttcctgg gcccgatgat ggatggctgg
4620gtcaagttcg gctcagtcat ggcggcactt gcgcatggtc tagcgctcat
aggcatgctc 4680acgttcttcg agttcttctg gttcctcgag ctctgggatg
cctcgcacgc cgtgctcggc 4740gtcatcgcca ttattgccgt tcagcgcggg
atccagaaga tcctcattgc cgtcttcctg 4800acgcgtgagt acaagcacga
cgagacgaac cgcgcgtggt ggacaggtaa atggtatgga 4860cgcgggctgg
gtacctcggc catgtcccag ccggcgcgcg agttcatcgt gaagatcgtg
4920gagatgtcgc tgtggacgtc ggacttcctg cttgcgcacc tgttgctcat
catcttgacg 4980gtgccgctac tgctgccgtt cttcaactcg atccattcga
cgatgctttt ctggttgcgc 5040ccttcgaagc agattaggca acctctgttc
tccactaagc agaagcggca acggcgatgg 5100attgtcatga agtataccgt
ggtatatctc gtggtggtgg ctttcctcgt tgcgctcatc 5160gctctgcccg
cgctcttccg cgagagcatc cacttcaact gcgagatctg ccagagtata 5220tag
522361740PRTSchizophyllum commune 6Met Ser Gly Pro Gly Tyr Gly Arg
Asn Pro Phe Asp Asn Pro Pro Pro 1 5 10 15 Asn Arg Gly Pro Tyr Gly
Gln Gln Pro Gly Phe Pro Gly Pro Gly Pro 20 25 30 Arg Pro Tyr Asp
Ser Asp Ala Asp Met Ser Gln Thr Tyr Gly Ser Thr 35 40 45 Thr Arg
Leu Ala Gly Ser Ala Gly Tyr Ser Asp Arg Asn Gly Ser Phe 50 55 60
Asp Gly Asp Arg Ser Tyr Ala Pro Ser Ile Asp Ser Arg Ala Ser Val 65
70 75 80 Pro Ser Ile Ser Pro Phe Ala Asp Pro Gly Ile Gly Ser Asn
Glu Pro 85 90 95 Tyr Pro Ala Trp Ser Val Glu Arg Gln Ile Pro Met
Ser Thr Glu Glu 100 105 110 Ile Glu Asp Ile Phe Leu Asp Leu Thr Gln
Lys Phe Gly Phe Gln Arg 115 120 125 Asp Ser Met Arg Asn Thr Phe Asp
Phe Met Met His Leu Leu Asp Ser 130 135 140 Arg Ala Ser Arg Met Thr
Pro Asn Gln Ala Leu Leu Thr Leu His Ala 145 150 155 160 Asp Tyr Ile
Gly Gly Gln His Ala Asn Tyr Arg Lys Trp Tyr Phe Ala 165 170 175 Ala
Gln Leu Asn Leu Asp Asp Ala Val Gly Gln Thr Asn Asn Pro Gly 180 185
190 Ile Gln Arg Leu Lys Thr Ile Lys Gly Ala Thr Lys Thr Lys Ser Leu
195 200 205 Asp Ser Ala Leu Asn Arg Trp Arg Asn Ala Met Asn Asn Met
Ser Gln 210 215 220 Tyr Asp Arg Leu Arg Gln Ile Ala Leu Tyr Leu Leu
Cys Trp Gly Glu 225 230 235 240 Ala Gly Asn Ile Arg Leu Ala Pro Glu
Cys Leu Cys Phe Ile Phe Lys 245 250 255 Cys Ala Asp Asp Tyr Tyr Arg
Ser Pro Glu Cys Gln Asn Arg Met Asp 260 265 270 Pro Val Pro Glu Gly
Leu Tyr Leu Gln Thr Val Ile Lys Pro Leu Tyr 275 280 285 Arg Phe Leu
Arg Asp Gln Ala Tyr Glu Val Val Asp Gly Lys Gln Val 290 295 300 Lys
Arg Glu Lys Asp His Asp Gln Ile Ile Gly Tyr Asp Asp Val Asn 305 310
315 320 Gln Leu Phe Trp Tyr Pro Glu Gly Leu Ala Lys Ile Val Met Ser
Asp 325 330 335 Asn Thr Arg Leu Val Asp Val Pro Pro Ala Gln Arg Phe
Met Lys Phe 340 345 350 Ala Lys Ile Glu Trp Asn Arg Val Phe Phe Lys
Thr Tyr Phe Glu Lys 355 360 365 Arg Ser Thr Ala His Leu Leu Val Asn
Phe Asn Arg Ile Trp Ile Leu 370 375 380 His Val Ser Met Tyr Phe Phe
Tyr Thr Ala Phe Asn Ser Pro Arg Val 385 390 395 400 Tyr Ala Pro His
Gly Lys Leu Asp Pro Ser Pro Glu Met Thr Trp Ser 405 410 415 Ala Thr
Ala Leu Gly Gly Ala Val Ser Thr Met Ile Met Ile Leu Ala 420 425 430
Thr Ile Ala Glu Tyr Thr Tyr Ile Pro Thr Thr Trp Asn Asn Ala Ser 435
440 445 His Leu Thr Thr Arg Leu Ile Phe Leu Leu Val Ile Leu Ala Leu
Thr 450 455 460 Ala Gly Pro Thr Phe Tyr Ile Ala Met Ile Asp Gly Arg
Thr Asp Ile 465 470 475 480 Gly Gln Val Pro Leu Ile Val Ala Ile Val
Gln Phe Phe Ile Ser Val 485 490 495 Val Ala Thr Leu Ala Phe Ala Thr
Ile Pro Ser Gly Arg Met Phe Gly 500 505 510 Asp Arg Val Ala Gly Lys
Ser Arg Lys His Met Ala Ser Gln Thr Phe 515 520 525 Thr Ala Ser Tyr
Pro Ser Met Lys Arg Ser Ser Arg Val Ala Ser Ile 530 535 540 Met Leu
Trp Leu Leu Val Phe Gly Cys Lys Tyr Val Glu Ser Tyr Phe 545 550 555
560 Phe Leu Thr Ser Ser Phe Ser Ser Pro Ile Ala Val Met Ala Arg Thr
565 570 575 Lys Val Gln Gly Cys Asn Asp Arg Ile Phe Gly Ser Gln Leu
Cys Thr 580 585 590 Asn Gln Val Pro Phe Ala Leu Ala Ile Met Tyr Val
Met Asp Leu Val 595 600 605 Leu Phe Phe Leu Asp Thr Tyr Leu Trp Tyr
Ile Ile Trp Leu Val Ile 610 615 620 Phe Ser Met Val Arg Ala Phe Lys
Leu Gly Ile Ser Ile Trp Thr Pro 625 630 635 640 Trp Ser Glu Ile Phe
Thr Arg Met Pro Lys Arg Ile Tyr Ala Lys Leu 645 650 655 Leu Ala Thr
Ala Glu Met Glu Val Lys Tyr Lys Pro Lys Val Leu Val 660 665 670 Ser
Gln Ile Trp Asn Ala Val Ile Ile Ser Met Tyr Arg Glu His Leu 675 680
685 Leu Ser Ile Glu His Val Gln Arg Leu Leu Tyr His Gln Val Asp Gly
690 695 700 Pro Asp Gly Arg Arg Thr Leu Arg Ala Pro Pro Phe Phe Thr
Ser Gln 705 710 715 720 Arg Thr Ala Lys Pro Gly Leu Phe Phe Pro Pro
Gly Gly Glu Ala Glu 725 730 735 Arg Arg Ile Ser Phe Phe Ala Ser Ser
Leu Thr Thr Ala Leu Pro Glu 740 745 750 Pro Leu Pro Ile Asp Ala Met
Pro Thr Phe Thr Val Leu Val Pro His 755 760 765 Tyr Ser Glu Lys Ile
Leu Leu Ser Leu Arg Glu Ile Ile Arg Glu Glu 770 775 780 Asp Gln Asn
Thr Arg Val Thr Leu Leu Glu Tyr Leu Lys Gln Leu His 785 790 795 800
Pro Val Glu Trp Asp Asn Phe Val Lys Asp Thr Lys Ile Leu Ala Glu 805
810 815 Glu Ser Gly Asp Val Gln Asp Glu Lys Arg
Ala Arg Thr Asp Asp Leu 820 825 830 Pro Phe Tyr Cys Ile Gly Phe Lys
Thr Ser Ser Pro Glu Tyr Thr Leu 835 840 845 Arg Thr Arg Ile Trp Ala
Ser Leu Arg Ala Gln Thr Leu Tyr Arg Thr 850 855 860 Val Ser Gly Met
Met Asn Tyr Ser Lys Ala Ile Lys Leu Leu Tyr Arg 865 870 875 880 Val
Glu Asn Pro Asp Val Val His Ala Phe Gly Gly Asn Thr Glu Arg 885 890
895 Leu Glu Arg Glu Leu Glu Arg Met Ser Arg Arg Lys Phe Lys Phe Val
900 905 910 Ile Ser Met Gln Arg Tyr Ser Lys Phe Asn Lys Glu Glu Gln
Glu Asn 915 920 925 Ala Glu Phe Leu Leu Arg Ala Tyr Pro Asp Leu Gln
Ile Ala Tyr Leu 930 935 940 Asp Glu Glu Pro Gly Pro Ser Lys Ser Asp
Glu Val Arg Leu Phe Ser 945 950 955 960 Thr Leu Ile Asp Gly His Ser
Glu Val Asp Glu Lys Thr Gly Arg Arg 965 970 975 Lys Pro Lys Phe Arg
Ile Glu Leu Pro Gly Asn Pro Ile Leu Gly Asp 980 985 990 Gly Lys Ser
Asp Asn Gln Asn His Ala Ile Val Phe Tyr Arg Gly Glu 995 1000 1005
Tyr Ile Gln Val Ile Asp Ala Asn Gln Asp Asn Tyr Leu Glu Glu 1010
1015 1020 Cys Leu Lys Ile Arg Asn Val Leu Gly Glu Phe Glu Glu Tyr
Ser 1025 1030 1035 Val Ser Ser Gln Ser Pro Tyr Ala Gln Trp Gly His
Lys Glu Phe 1040 1045 1050 Asn Lys Cys Pro Val Ala Ile Leu Gly Ser
Arg Glu Tyr Ile Phe 1055 1060 1065 Ser Glu Asn Ile Gly Ile Leu Gly
Asp Ile Ala Ala Gly Lys Glu 1070 1075 1080 Gln Thr Phe Gly Thr Ile
Thr Ala Arg Ala Leu Ala Trp Ile Gly 1085 1090 1095 Gly Lys Leu His
Tyr Gly His Pro Asp Phe Leu Asn Ala Thr Phe 1100 1105 1110 Met Thr
Thr Arg Gly Gly Val Ser Lys Ala Gln Lys Gly Leu His 1115 1120 1125
Leu Asn Glu Asp Ile Phe Ala Gly Met Thr Ala Val Ser Arg Gly 1130
1135 1140 Gly Arg Ile Lys His Met Glu Tyr Tyr Gln Cys Gly Lys Gly
Arg 1145 1150 1155 Asp Leu Gly Phe Gly Thr Ile Leu Asn Phe Gln Thr
Lys Ile Gly 1160 1165 1170 Thr Gly Met Gly Glu Gln Leu Leu Ser Arg
Glu Tyr Tyr Tyr Leu 1175 1180 1185 Gly Thr Gln Leu Pro Ile Asp Arg
Phe Leu Thr Phe Tyr Tyr Ala 1190 1195 1200 His Ala Gly Phe His Val
Asn Asn Ile Leu Val Ile Tyr Ser Ile 1205 1210 1215 Gln Val Phe Met
Val Thr Leu Leu Tyr Leu Gly Thr Leu Asn Lys 1220 1225 1230 Gln Leu
Phe Ile Cys Lys Val Asn Ser Asn Gly Gln Val Leu Ser 1235 1240 1245
Gly Gln Ala Gly Cys Tyr Asn Leu Ile Pro Val Phe Glu Trp Ile 1250
1255 1260 Arg Arg Ser Ile Ile Ser Ile Phe Leu Val Phe Phe Ile Ala
Phe 1265 1270 1275 Leu Pro Leu Phe Leu Gln Glu Leu Cys Glu Arg Gly
Thr Gly Lys 1280 1285 1290 Ala Leu Leu Arg Leu Gly Lys His Phe Leu
Ser Leu Ser Pro Ile 1295 1300 1305 Phe Glu Val Phe Ser Thr Gln Ile
Tyr Ser Gln Ala Leu Leu Asn 1310 1315 1320 Asn Met Ser Phe Gly Gly
Ala Arg Tyr Ile Ala Thr Gly Arg Gly 1325 1330 1335 Phe Ala Thr Ser
Arg Ile Pro Phe Asn Ile Leu Tyr Ser Arg Phe 1340 1345 1350 Ala Pro
Pro Ser Ile Tyr Met Gly Met Arg Asn Leu Leu Leu Leu 1355 1360 1365
Leu Tyr Ala Thr Met Ala Ile Trp Ile Pro His Leu Ile Tyr Phe 1370
1375 1380 Trp Phe Ser Val Leu Ser Leu Cys Ile Ala Pro Phe Met Phe
Asn 1385 1390 1395 Pro His Gln Phe Ser Tyr Ala Asp Phe Ile Ile Asp
Tyr Arg Glu 1400 1405 1410 Phe Leu Arg Trp Met Ser Arg Gly Asn Ser
Arg Thr Lys Ala Ser 1415 1420 1425 Ser Trp Tyr Gly Tyr Cys Arg Leu
Ser Arg Thr Ala Ile Thr Gly 1430 1435 1440 Tyr Lys Lys Lys Lys Leu
Gly His Pro Ser Glu Lys Leu Ser Gly 1445 1450 1455 Asp Val Pro Arg
Ala Pro Trp Arg Asn Val Ile Phe Ser Glu Ile 1460 1465 1470 Leu Trp
Pro Ile Gly Ala Cys Ile Ile Phe Ile Val Ala Tyr Met 1475 1480 1485
Phe Val Lys Ser Phe Pro Asp Glu Gln Gly Asn Ala Pro Pro Ser 1490
1495 1500 Pro Leu Val Arg Ile Leu Leu Ile Ala Val Gly Pro Thr Val
Trp 1505 1510 1515 Asn Ala Ala Val Leu Ile Thr Leu Phe Phe Leu Ser
Leu Phe Leu 1520 1525 1530 Gly Pro Met Met Asp Gly Trp Val Lys Phe
Gly Ser Val Met Ala 1535 1540 1545 Ala Leu Ala His Gly Leu Ala Leu
Ile Gly Met Leu Thr Phe Phe 1550 1555 1560 Glu Phe Phe Trp Phe Leu
Glu Leu Trp Asp Ala Ser His Ala Val 1565 1570 1575 Leu Gly Val Ile
Ala Ile Ile Ala Val Gln Arg Gly Ile Gln Lys 1580 1585 1590 Ile Leu
Ile Ala Val Phe Leu Thr Arg Glu Tyr Lys His Asp Glu 1595 1600 1605
Thr Asn Arg Ala Trp Trp Thr Gly Lys Trp Tyr Gly Arg Gly Leu 1610
1615 1620 Gly Thr Ser Ala Met Ser Gln Pro Ala Arg Glu Phe Ile Val
Lys 1625 1630 1635 Ile Val Glu Met Ser Leu Trp Thr Ser Asp Phe Leu
Leu Ala His 1640 1645 1650 Leu Leu Leu Ile Ile Leu Thr Val Pro Leu
Leu Leu Pro Phe Phe 1655 1660 1665 Asn Ser Ile His Ser Thr Met Leu
Phe Trp Leu Arg Pro Ser Lys 1670 1675 1680 Gln Ile Arg Gln Pro Leu
Phe Ser Thr Lys Gln Lys Arg Gln Arg 1685 1690 1695 Arg Trp Ile Val
Met Lys Tyr Thr Val Val Tyr Leu Val Val Val 1700 1705 1710 Ala Phe
Leu Val Ala Leu Ile Ala Leu Pro Ala Leu Phe Arg Glu 1715 1720 1725
Ser Ile His Phe Asn Cys Glu Ile Cys Gln Ser Ile 1730 1735 1740
74869DNASchizophyllum commune 7atgcggaaca tgttcgactt caccatgcag
ctgcttgaca gccgagcgtc tcgtatgacc 60cccaaccagg cgctcctcac cctccacgcc
gactacattg gtggccagca tgcgaactac 120cggaagtggt acttcgcggc
gcagctcgac cttgacgacg ccgtgggaca aactcagaat 180ccgggtctca
accgcctcaa gtccactcgc ggatcgggca agcgaccacg ccatgaaaag
240tcgctgaaca cggcattgga gcgctggcgg caagccatga acaacatgtc
gcagtatgac 300cgcttacgcc agatcgcgct ctacctgctc tgctggggcg
aagcggcgca agtgcgattc 360atgcccgagt gcttgtgctt catcttcaag
tgcgccgacg actattatcg ttcgccggag 420tgccagaaca ggatggagcc
ggtaccggag ggtctctacc tgaggacggt cgtaaagccg 480ctctacagat
ttgtccggga tcaaggctat gaggtggtgg agggaaaatt cgtacggcgg
540gaacgggatc acgaccaaat cattggttac gatgacgtga atcagctgtt
ctggtacccg 600gagggcattg cccgtatcgt cctgtcggac aagagtcgtc
tggtcgacct ccctccagca 660cagcgcttca tgaagttcga ccgtatcgag
tggaatcgcg tcttcttcaa gacgttctac 720gagactcgat cctttacgca
tcttttggtc gacttcaacc gtatctgggt cgtgcacatc 780gctctctact
tcttctacac cgcatacaac tcccccacga tctacgccat caacggcaac
840actccgacgt ctctggcttg gagcgcgact gcgctcggcg gtgcggtagc
gacaggtatc 900atgatcctcg ccacgatcgc cgagttctcg cacatcccca
cgacatggaa caacacctcg 960catctgactc gccgcctcgc cttcctcctc
gtcacgctcg gcctcacatg tggtccgacg 1020ttctacgtcg cgattgcaga
gagcaacggg agcggcggct ctttggcctt gattctcggc 1080atcgtccagt
tcttcatctc cgtcgtagcg actgcgctct tcactatcat gccttctggt
1140cgtatgttcg gcgaccgcgt cgcaggcaag agtcgcaagt atctcgccag
ccagacgttc 1200acggccagct acccgtcgtt gcccaagcac cagcggttcg
catcactcct gatgtggttc 1260ctcatcttcg ggtgcaagtt gacggagagt
tacttcttcc tgacgttgtc cttccgcgac 1320cctattcgcg tcatggtcgg
catgaagatc cagaactgcg aggacaagat tttcggcagc 1380ggcctttgca
ggaatcacgc agcattcacc ctcacgatca tgtacatcat ggacctcgtc
1440ttgttcttcc tcgacacctt cctttggtat gtcatctgga actcggtttt
cagtatcgca 1500cgctctttcg tactcggcct ttcgatctgg acaccatgga
gggacatctt ccagcgtctg 1560ccgaagcgta tctacgcgaa gcttctagcg
accggcgaca tggaggtcaa gtacaagccc 1620aaggtcttgg tttcgcaaat
ctggaacgcc atcatcatct ccatgtaccg cgagcacttg 1680ctctctatcg
agcacgttca aaagctcctg taccatcaag tggacactgg cgaagccggc
1740aagcggagtc ttcgcgcgcc tccgttcttc gtcgcgcagg gcagcagcgg
tggctcgggc 1800gagttcttcc cgcctggtag cgaggctgag cgtcgtatct
ctttcttcgc gcagtctcta 1860tctacggaga ttcctcagcc catcccggtt
gacgccatgc cgacgttcac agtgcttacg 1920cctcactaca gcgagaagat
ccttctttcg ctccgtgaga ttatccgcga ggaggaccag 1980aacacccgcg
tgacattgct tgagtatctc aagcagcttc acccggtcga gtgggagaac
2040ttcgtcaagg acaccaagat tttggccgag gagtccgcta tgttcaacgg
tccaagtcct 2100ttcggcaacg atgagaaggg tcagtccaag atggacgatc
ttcctttcta ctgcatcggt 2160ttcaagagcg ccgcgcccga gtacaccctc
cgcacccgta tctgggcgtc cttgcgcgcg 2220cagaccctct accgcacggt
ctccggcatg atgaactatg cgaaggcgat taagctgctc 2280taccgcgtcg
agaaccccga ggtcgtgcag cagttcggcg gtaacacgga caagctcgag
2340cgcgagttgg agcggatggc ccggcggaag ttcaagttcc tggtgtccat
gcagcgctac 2400tcgaagttca acaaggagga gcacgagaac gccgagttct
tgctccgcgc gtacccggac 2460ctgcagatcg cgtacctgga ggaagagcct
cctcgcaagg agggtggcga tccacgcatc 2520ttctctgccc tcgtcgacgg
ccacagcgac atcatcccgg agaccggcaa gcggcgcccc 2580aagttccgca
tcgagctgcc cggcaacccc attctcggtg acggcaagtc ggacaaccag
2640aaccacgcca tcgtcttcta ccgcggcgag tacctccagc ttatcgacgc
caaccaggac 2700aactacctcg aggagtgctt gaagatccgt aacgtactcg
ccgagttcga ggagtacgac 2760gtctctagcc agagtccgta cgcgcagtgg
agtgtcaagg agttcaagcg ctccccggtc 2820gccatcgtcg gtgcacgcga
gtatatcttc tcggagcaca tcggtattct cggtgatttg 2880gcggctggca
aggaacagac gttcggtacg ctcacggcac gcaacaacgc cttccttggc
2940ggcaagctgc actacggtca cccggatttc ctcaacgccc tctacatgaa
cacgcgcggt 3000ggtgtctcca aggcgcagaa gggtctccat ctcaacgagg
atatttacgc cggtatgaac 3060gcggtcggtc gcggtggacg catcaagcat
agcgaatact accagtgcgg caagggtcgt 3120gacctcggtt ttggcaccat
cttgaacttc cagaccaaga tcggtacggg tatgggcgag 3180cagatcctct
cgcgcgagta ctactacctc ggaacccaat tgcccatcga tcgcttcctc
3240acgttctact acgcgcaccc aggtttccag atcaacaaca tgctggttat
cctatccgtg 3300caggtcttca tcgttaccat ggtcttcctc ggtaccttga
agtcttcggt cacgatctgc 3360aagtacacgt ccagcggtca gtacatcggt
ggtcaatccg gttgctacaa cctcgtcccg 3420gtcttccagt ggatcgagcg
ctgcatcatc agcatcttct tggtgttcat gatcgctttc 3480atgccgctct
tcctgcaaga actcgtcgag cgcggtacct ggagtgccat ctggcgtctg
3540ctcaagcagt ttatgtcgct gtcgcctgtc ttcgaggtgt tctccaccca
gattcagaca 3600cactccgtgt tgagcaactt gacgttcggt ggtgcgcgtt
acatcgctac cggtcgtggg 3660ttcgccacca gtcgtatcag cttcagcatc
ttgttctcgc gtttcgcagg cccgagtatc 3720tacctcggca tgcgcacgct
cattatgctg ctctacgtga cgttgacgat ctggacgcca 3780tgggtcattt
acttctgggt ttccattctc tcgctctgca tcgcgccgtt cttgttcaat
3840ccgcatcaat tcgtcttctc ggatttcctc atcgactaca gggaatacct
ccggtggatg 3900tcgcgtggta actcgcgctc gcacaacaac tcctggattg
ggtactgccg gttgtcccgc 3960acgatgatca ctgggtacaa gaagaagaag
ctgggccacc cgtcggagaa gctttccggc 4020gacgttcctc gtgcaggctg
gcgcgccgtc ttattctcgg agatcatctt cccggcatgc 4080atggccatcc
tcttcatcat cgcgtacatg ttcgtcaagt cgttccctct cgacggcaag
4140cagcctccct ccggcctcgt tcgcatcgcc gtcgtgtcta tcggccccat
cgtgtggaac 4200gccgccatcc tgttgacgct cttccttgtg tcgttgttcc
tcggccccat gctcgacccg 4260gtcttccccc tcttcggttc cgttatggcc
ttcatcgcgc atttcctcgg cacaatcgga 4320atgattgggt tcttcgagtt
cctgtggttc ctcgagtcct gggaggcgtc gcatgccgtg 4380ctgggtctca
tcgccgtcat ctccatccag cgcgccattc acaaaattct tatcgccgtt
4440ttcctcagtc gcgagttcaa gcacgacgag acgaacaggg cttggtggac
tggtcgctgg 4500tatggccgtg gcctcggcac gcacgccatg tcgcagccgg
cgcgtgagtt cgtcgtcaag 4560atcatcgagt tgtcgctctg gagctcggat
ctcatactcg gccacatcct gctgttcatg 4620cttactccgg ctgtcctcat
cccgtacttc gaccgtctgc acgccatgat gctcttctgg 4680ctgcgcccct
caaagcaaat ccgcgcgcct ctgtactcaa tcaagcagaa gaggcaaaga
4740cgctggatta tcatgaagta cggtactgta tacgttaccg tcatcgcgat
cttcgtcgcg 4800ctcatcgcgc ttcccctcgt cttccgacac actctaaagg
tcgagtgctc cctttgcgac 4860agcttgtaa 486981622PRTSchizophyllum
commune 8Met Arg Asn Met Phe Asp Phe Thr Met Gln Leu Leu Asp Ser
Arg Ala 1 5 10 15 Ser Arg Met Thr Pro Asn Gln Ala Leu Leu Thr Leu
His Ala Asp Tyr 20 25 30 Ile Gly Gly Gln His Ala Asn Tyr Arg Lys
Trp Tyr Phe Ala Ala Gln 35 40 45 Leu Asp Leu Asp Asp Ala Val Gly
Gln Thr Gln Asn Pro Gly Leu Asn 50 55 60 Arg Leu Lys Ser Thr Arg
Gly Ser Gly Lys Arg Pro Arg His Glu Lys 65 70 75 80 Ser Leu Asn Thr
Ala Leu Glu Arg Trp Arg Gln Ala Met Asn Asn Met 85 90 95 Ser Gln
Tyr Asp Arg Leu Arg Gln Ile Ala Leu Tyr Leu Leu Cys Trp 100 105 110
Gly Glu Ala Ala Gln Val Arg Phe Met Pro Glu Cys Leu Cys Phe Ile 115
120 125 Phe Lys Cys Ala Asp Asp Tyr Tyr Arg Ser Pro Glu Cys Gln Asn
Arg 130 135 140 Met Glu Pro Val Pro Glu Gly Leu Tyr Leu Arg Thr Val
Val Lys Pro 145 150 155 160 Leu Tyr Arg Phe Val Arg Asp Gln Gly Tyr
Glu Val Val Glu Gly Lys 165 170 175 Phe Val Arg Arg Glu Arg Asp His
Asp Gln Ile Ile Gly Tyr Asp Asp 180 185 190 Val Asn Gln Leu Phe Trp
Tyr Pro Glu Gly Ile Ala Arg Ile Val Leu 195 200 205 Ser Asp Lys Ser
Arg Leu Val Asp Leu Pro Pro Ala Gln Arg Phe Met 210 215 220 Lys Phe
Asp Arg Ile Glu Trp Asn Arg Val Phe Phe Lys Thr Phe Tyr 225 230 235
240 Glu Thr Arg Ser Phe Thr His Leu Leu Val Asp Phe Asn Arg Ile Trp
245 250 255 Val Val His Ile Ala Leu Tyr Phe Phe Tyr Thr Ala Tyr Asn
Ser Pro 260 265 270 Thr Ile Tyr Ala Ile Asn Gly Asn Thr Pro Thr Ser
Leu Ala Trp Ser 275 280 285 Ala Thr Ala Leu Gly Gly Ala Val Ala Thr
Gly Ile Met Ile Leu Ala 290 295 300 Thr Ile Ala Glu Phe Ser His Ile
Pro Thr Thr Trp Asn Asn Thr Ser 305 310 315 320 His Leu Thr Arg Arg
Leu Ala Phe Leu Leu Val Thr Leu Gly Leu Thr 325 330 335 Cys Gly Pro
Thr Phe Tyr Val Ala Ile Ala Glu Ser Asn Gly Ser Gly 340 345 350 Gly
Ser Leu Ala Leu Ile Leu Gly Ile Val Gln Phe Phe Ile Ser Val 355 360
365 Val Ala Thr Ala Leu Phe Thr Ile Met Pro Ser Gly Arg Met Phe Gly
370 375 380 Asp Arg Val Ala Gly Lys Ser Arg Lys Tyr Leu Ala Ser Gln
Thr Phe 385 390 395 400 Thr Ala Ser Tyr Pro Ser Leu Pro Lys His Gln
Arg Phe Ala Ser Leu 405 410 415 Leu Met Trp Phe Leu Ile Phe Gly Cys
Lys Leu Thr Glu Ser Tyr Phe 420 425 430 Phe Leu Thr Leu Ser Phe Arg
Asp Pro Ile Arg Val Met Val Gly Met 435 440 445 Lys Ile Gln Asn Cys
Glu Asp Lys Ile Phe Gly Ser Gly Leu Cys Arg 450 455 460 Asn His Ala
Ala Phe Thr Leu Thr Ile Met Tyr Ile Met Asp Leu Val 465 470 475 480
Leu Phe Phe Leu Asp Thr Phe Leu Trp Tyr Val Ile Trp Asn Ser Val 485
490 495 Phe Ser Ile Ala Arg Ser Phe Val Leu Gly Leu Ser Ile Trp Thr
Pro 500 505 510 Trp Arg Asp Ile Phe Gln Arg Leu Pro Lys Arg Ile Tyr
Ala Lys Leu 515 520 525 Leu Ala Thr Gly Asp Met Glu Val Lys Tyr Lys
Pro Lys Val Leu Val 530 535 540 Ser Gln Ile Trp Asn Ala Ile Ile Ile
Ser Met Tyr Arg Glu His Leu 545 550 555 560 Leu Ser Ile Glu His Val
Gln Lys Leu Leu Tyr His Gln Val Asp Thr 565 570 575 Gly Glu Ala Gly
Lys Arg Ser Leu Arg Ala Pro Pro Phe Phe Val Ala 580 585
590 Gln Gly Ser Ser Gly Gly Ser Gly Glu Phe Phe Pro Pro Gly Ser Glu
595 600 605 Ala Glu Arg Arg Ile Ser Phe Phe Ala Gln Ser Leu Ser Thr
Glu Ile 610 615 620 Pro Gln Pro Ile Pro Val Asp Ala Met Pro Thr Phe
Thr Val Leu Thr 625 630 635 640 Pro His Tyr Ser Glu Lys Ile Leu Leu
Ser Leu Arg Glu Ile Ile Arg 645 650 655 Glu Glu Asp Gln Asn Thr Arg
Val Thr Leu Leu Glu Tyr Leu Lys Gln 660 665 670 Leu His Pro Val Glu
Trp Glu Asn Phe Val Lys Asp Thr Lys Ile Leu 675 680 685 Ala Glu Glu
Ser Ala Met Phe Asn Gly Pro Ser Pro Phe Gly Asn Asp 690 695 700 Glu
Lys Gly Gln Ser Lys Met Asp Asp Leu Pro Phe Tyr Cys Ile Gly 705 710
715 720 Phe Lys Ser Ala Ala Pro Glu Tyr Thr Leu Arg Thr Arg Ile Trp
Ala 725 730 735 Ser Leu Arg Ala Gln Thr Leu Tyr Arg Thr Val Ser Gly
Met Met Asn 740 745 750 Tyr Ala Lys Ala Ile Lys Leu Leu Tyr Arg Val
Glu Asn Pro Glu Val 755 760 765 Val Gln Gln Phe Gly Gly Asn Thr Asp
Lys Leu Glu Arg Glu Leu Glu 770 775 780 Arg Met Ala Arg Arg Lys Phe
Lys Phe Leu Val Ser Met Gln Arg Tyr 785 790 795 800 Ser Lys Phe Asn
Lys Glu Glu His Glu Asn Ala Glu Phe Leu Leu Arg 805 810 815 Ala Tyr
Pro Asp Leu Gln Ile Ala Tyr Leu Glu Glu Glu Pro Pro Arg 820 825 830
Lys Glu Gly Gly Asp Pro Arg Ile Phe Ser Ala Leu Val Asp Gly His 835
840 845 Ser Asp Ile Ile Pro Glu Thr Gly Lys Arg Arg Pro Lys Phe Arg
Ile 850 855 860 Glu Leu Pro Gly Asn Pro Ile Leu Gly Asp Gly Lys Ser
Asp Asn Gln 865 870 875 880 Asn His Ala Ile Val Phe Tyr Arg Gly Glu
Tyr Leu Gln Leu Ile Asp 885 890 895 Ala Asn Gln Asp Asn Tyr Leu Glu
Glu Cys Leu Lys Ile Arg Asn Val 900 905 910 Leu Ala Glu Phe Glu Glu
Tyr Asp Val Ser Ser Gln Ser Pro Tyr Ala 915 920 925 Gln Trp Ser Val
Lys Glu Phe Lys Arg Ser Pro Val Ala Ile Val Gly 930 935 940 Ala Arg
Glu Tyr Ile Phe Ser Glu His Ile Gly Ile Leu Gly Asp Leu 945 950 955
960 Ala Ala Gly Lys Glu Gln Thr Phe Gly Thr Leu Thr Ala Arg Asn Asn
965 970 975 Ala Phe Leu Gly Gly Lys Leu His Tyr Gly His Pro Asp Phe
Leu Asn 980 985 990 Ala Leu Tyr Met Asn Thr Arg Gly Gly Val Ser Lys
Ala Gln Lys Gly 995 1000 1005 Leu His Leu Asn Glu Asp Ile Tyr Ala
Gly Met Asn Ala Val Gly 1010 1015 1020 Arg Gly Gly Arg Ile Lys His
Ser Glu Tyr Tyr Gln Cys Gly Lys 1025 1030 1035 Gly Arg Asp Leu Gly
Phe Gly Thr Ile Leu Asn Phe Gln Thr Lys 1040 1045 1050 Ile Gly Thr
Gly Met Gly Glu Gln Ile Leu Ser Arg Glu Tyr Tyr 1055 1060 1065 Tyr
Leu Gly Thr Gln Leu Pro Ile Asp Arg Phe Leu Thr Phe Tyr 1070 1075
1080 Tyr Ala His Pro Gly Phe Gln Ile Asn Asn Met Leu Val Ile Leu
1085 1090 1095 Ser Val Gln Val Phe Ile Val Thr Met Val Phe Leu Gly
Thr Leu 1100 1105 1110 Lys Ser Ser Val Thr Ile Cys Lys Tyr Thr Ser
Ser Gly Gln Tyr 1115 1120 1125 Ile Gly Gly Gln Ser Gly Cys Tyr Asn
Leu Val Pro Val Phe Gln 1130 1135 1140 Trp Ile Glu Arg Cys Ile Ile
Ser Ile Phe Leu Val Phe Met Ile 1145 1150 1155 Ala Phe Met Pro Leu
Phe Leu Gln Glu Leu Val Glu Arg Gly Thr 1160 1165 1170 Trp Ser Ala
Ile Trp Arg Leu Leu Lys Gln Phe Met Ser Leu Ser 1175 1180 1185 Pro
Val Phe Glu Val Phe Ser Thr Gln Ile Gln Thr His Ser Val 1190 1195
1200 Leu Ser Asn Leu Thr Phe Gly Gly Ala Arg Tyr Ile Ala Thr Gly
1205 1210 1215 Arg Gly Phe Ala Thr Ser Arg Ile Ser Phe Ser Ile Leu
Phe Ser 1220 1225 1230 Arg Phe Ala Gly Pro Ser Ile Tyr Leu Gly Met
Arg Thr Leu Ile 1235 1240 1245 Met Leu Leu Tyr Val Thr Leu Thr Ile
Trp Thr Pro Trp Val Ile 1250 1255 1260 Tyr Phe Trp Val Ser Ile Leu
Ser Leu Cys Ile Ala Pro Phe Leu 1265 1270 1275 Phe Asn Pro His Gln
Phe Val Phe Ser Asp Phe Leu Ile Asp Tyr 1280 1285 1290 Arg Glu Tyr
Leu Arg Trp Met Ser Arg Gly Asn Ser Arg Ser His 1295 1300 1305 Asn
Asn Ser Trp Ile Gly Tyr Cys Arg Leu Ser Arg Thr Met Ile 1310 1315
1320 Thr Gly Tyr Lys Lys Lys Lys Leu Gly His Pro Ser Glu Lys Leu
1325 1330 1335 Ser Gly Asp Val Pro Arg Ala Gly Trp Arg Ala Val Leu
Phe Ser 1340 1345 1350 Glu Ile Ile Phe Pro Ala Cys Met Ala Ile Leu
Phe Ile Ile Ala 1355 1360 1365 Tyr Met Phe Val Lys Ser Phe Pro Leu
Asp Gly Lys Gln Pro Pro 1370 1375 1380 Ser Gly Leu Val Arg Ile Ala
Val Val Ser Ile Gly Pro Ile Val 1385 1390 1395 Trp Asn Ala Ala Ile
Leu Leu Thr Leu Phe Leu Val Ser Leu Phe 1400 1405 1410 Leu Gly Pro
Met Leu Asp Pro Val Phe Pro Leu Phe Gly Ser Val 1415 1420 1425 Met
Ala Phe Ile Ala His Phe Leu Gly Thr Ile Gly Met Ile Gly 1430 1435
1440 Phe Phe Glu Phe Leu Trp Phe Leu Glu Ser Trp Glu Ala Ser His
1445 1450 1455 Ala Val Leu Gly Leu Ile Ala Val Ile Ser Ile Gln Arg
Ala Ile 1460 1465 1470 His Lys Ile Leu Ile Ala Val Phe Leu Ser Arg
Glu Phe Lys His 1475 1480 1485 Asp Glu Thr Asn Arg Ala Trp Trp Thr
Gly Arg Trp Tyr Gly Arg 1490 1495 1500 Gly Leu Gly Thr His Ala Met
Ser Gln Pro Ala Arg Glu Phe Val 1505 1510 1515 Val Lys Ile Ile Glu
Leu Ser Leu Trp Ser Ser Asp Leu Ile Leu 1520 1525 1530 Gly His Ile
Leu Leu Phe Met Leu Thr Pro Ala Val Leu Ile Pro 1535 1540 1545 Tyr
Phe Asp Arg Leu His Ala Met Met Leu Phe Trp Leu Arg Pro 1550 1555
1560 Ser Lys Gln Ile Arg Ala Pro Leu Tyr Ser Ile Lys Gln Lys Arg
1565 1570 1575 Gln Arg Arg Trp Ile Ile Met Lys Tyr Gly Thr Val Tyr
Val Thr 1580 1585 1590 Val Ile Ala Ile Phe Val Ala Leu Ile Ala Leu
Pro Leu Val Phe 1595 1600 1605 Arg His Thr Leu Lys Val Glu Cys Ser
Leu Cys Asp Ser Leu 1610 1615 1620 9 6098DNASchizophyllum commune
9cccgtccctc aaggccgttc tttcgctggc gaccgacccg gtgttcgcga gaacctgttg
60tttctgacga tcatcaaccc tttcttctcg tcgctcttta gctctcccta gaccgtcttt
120tactctactc ttcgacgcac gccatgtccg gtccaggata tggcaggaat
ccattcgaca 180atcccccgcc caacagaggt ccctatggcc agcagccagg
tttcccgggg cccggccctc 240ggccttacga ctcggacgcg gacatgagcc
agacctatgg cagcacaacc aggctcgccg 300gcagtgccgg ttacagcgac
agaaacggtg cgaacgtcgc taccgtactt cctcgatcgt 360cgactcacat
atcacgcagg cagcttcgac ggcgaccgct cctacgcgcc ctcaattgac
420tcgcgcgcca gcgtgcccag catatcgccc ttcgcagacc cgggtatcgg
ctctaatgag 480ccgtatcccg cttggtcggt cgaacgccag atccccatgt
ccacggagga gattgaggat 540atcttcctcg acctcaccca aaagtttggc
ttccagcgcg actccatgcg gaatacggtg 600cgtgaataag cagcccactc
gaccgcggga acagctcaat tgacctgtca cccagttcga 660cttcatgatg
cacctccttg attcccgtgc ctcgcgcatg acgcccaacc aagctctgct
720cacgcttcac gccgactaca ttggtggcca gcacgccaac tataggaagt
ggtatttcgc 780cgctcagctc aacctcgatg acgcggtcgg gcaaaccaat
aaccccggta tccagcgctt 840gaagaccatc aagggcgcta cgaagaccaa
gtcgctcgac agcgcactca accgctggcg 900caatgcgatg aacaacatga
gccagtacga tcgcctccgg caaattgcgc tctatctcct 960ctgctgggga
gaagcaggca acatccgtct ggcgcccgag tgcttgtgct tcatcttcaa
1020gtgcgcggac gactactaca gaagtcccga gtgtcagaac cggatggacc
ccgtgccgga 1080agggctgtac ctccagacgg tcatcaagcc gctctatcgc
ttcctacgtg atcaggcgta 1140cgaagtcgtt gatgggaagc aagtgaagcg
cgagaaggac cacgaccaga ttatcggtta 1200tgacgacgtc aaccagttat
tctggtatcc ggaaggtttg gctaagatcg tcatgtcgga 1260caacgtgcgt
atgatcttat cggttacaat tcgtccgctc acatctttcc agacacgact
1320tgtagatgta cctccggcgc agcggttcat gaagttcgcc aagatcgagt
ggaaccgcgt 1380cttcttcaag acgtactttg agaagcgctc tactgcccat
ctcctggtca acttcaaccg 1440tatatggatc ctccacgtct cgatgtactt
cttctacacg gcattcaact ctccacgagt 1500ctacgcgccg cacggcaaac
tcgacccctc ccctgagatg acctggtccg cgactgccct 1560tggaggcgct
gtgtccacca tgatcatgat ccttgccact atcgcggagt acacctacat
1620ccccacgaca tggaacaatg cgtcgcacct caccacgcgg ctcattttcc
tcctggtcat 1680cctcgcgctc actgctggac caacattcta tatcgccatg
atagacggac gcacggacat 1740cggccaagta ccactcatcg tggccatagt
gcagttcttc atctccgtcg tcgccaccct 1800cgctttcgct accatccctt
ctggtcgcat gttcggcgac cgtgtggctg gcaagtcaag 1860aaagcacatg
gcatcgcaga cgttcacagc gtcgtacccg tccatgaagc ggtcatctcg
1920cgtagcgagt atcatgctgt ggcttttggt ctttggctgc aaatacgtcg
agtcttactt 1980cttcttgacg tcctccttct ccagcccgat cgcggtcatg
gcgcgtacga aggtacaggg 2040ctgcaacgac cgtatcttcg gcagccagct
gtgcacgaat caggtcccgt tcgcgctggc 2100aatcatgtac gtgatggacc
tggtactgtt cttcctggac acgtacctgt ggtacatcat 2160ctggctggtg
atcttctcga tggtgcgcgc gttcaagctt ggtatctcga tctggacgcc
2220ctggagcgag atcttcaccc gcatgccgaa gcgtatctac gcgaagctgc
tggcgacggc 2280cgagatggag gtcaagtata agcccaaggt atgctgaatg
caatctggtc aggtgaattc 2340accctcatat tgttgtgcag gtgctcgtct
cgcaaatctg gaacgcggtc atcatctcca 2400tgtaccggga gcatctcttg
tccatcgagc acgtccagcg cctgctatac caccaggttg 2460atggtccaga
cggtcgccgc accctcaggg caccgccgtt cttcaccagc cagcgaactg
2520cgaagccagg cctgttcttc cctcctggtg gcgaggctga gcgccgtatc
tcgttctttg 2580cctcatcgct gacgaccgcg ctccctgagc ctctgccgat
cgacgccatg cccaccttca 2640ccgtgctcgt tccccattac tcggagaaga
ttctgctcag tctgcgcgag attattcgcg 2700aggaggacca gaacacccgc
gtcaccttgc tggagtacct caagcagctc caccctgtcg 2760aatgggacaa
cttcgtcaag gacaccaaga tcttggcgga agagtcgggc gacgtccagg
2820acgagaagcg cgcgcgcacg gacgacttgc cgttctactg catcgggttc
aagacctcgt 2880caccagagta caccctgcgt acgcgtatct gggcttcact
gcgcgcacag acgctgtacc 2940gcacggtctc cggtatgatg aactactcca
aggcgatcaa gctcctctat cgcgtcgaga 3000acccggatgt cgttcatgcc
ttcggtggga acacggaacg tcttgaacgc gagcttgagc 3060gcatgtctcg
ccgcaagttc aagttcgtca tctcgatgca gcggtactct aagttcaaca
3120aggaggagca agagaacgcc gaattccttc tgcgcgcgta cccggatttg
cagatcgcgt 3180acctcgatga agagcccggt cccagcaaga gcgacgaggt
tcggttgttt tcgacactca 3240tcgatggaca ctccgaggtg gatgagaaga
ccggccgccg caagcccaag ttccgcattg 3300agctgcccgg taaccccatc
ctcggtgacg ggaagtcgga taaccagaac cacgccattg 3360tcttctaccg
cggcgagtac atccaggtca tcgacgctaa ccaggacaat tacctggaag
3420agtgtctcaa gatccgtaac gtcctgggcg agtttgagga atactccgtg
tcgagccaga 3480gcccgtacgc acagtggggc cacaaggagt tcaacaagtg
ccccgtcgct atcctgggtt 3540ctcgcgagta catcttctcg gagaacatcg
gtatcctcgg tgacatcgcc gccggcaagg 3600aacagacgtt cggtaccatt
acggcgcgtg cgcttgcgtg gatcggcggc aagctgcatt 3660acggtcaccc
ggatttcctc aatgcgacgt tcatgacgac gcgtggtggc gtgtcaaaag
3720cgcagaaggg cttgcatctc aacgaggata tcttcgctgg tatgaccgcc
gtgtcccgcg 3780gagggcgcat caagcacatg gagtactacc agtgcggcaa
aggtcgtgat ctcggtttcg 3840gcacgatctt gaacttccag acgaagatcg
gtactggtat gggcgagcag ctcctctcgc 3900gcgagtacta ctacctgggc
acgcaattgc ctatcgaccg gttcttgacg ttctactacg 3960cgcacgctgg
tttccacgtc aacaacatcc tggtcatcta ctccatccag gtcttcatgg
4020tcacctgtaa gtgcaggcgc tcatgaccgc cgagaacgta gtctgacgga
tgtgcagtgc 4080tgtacctggg cacattgaac aagcagctgt tcatctgcaa
ggtcaactcc aatggccagg 4140ttcttagtgg acaagctggg tgctacaacc
tcatcccggt cttcgagtgg attcgccgga 4200gtatcatctc catcttcttg
gtgttcttca tcgccttctt gcctctattc ttgcaaggta 4260tgttcacttt
ccatgtgtca tccgttagcc gctcaccata cgacagagct gtgcgagcgc
4320ggaacgggaa aggcgttgct gcgtctcggg aagcacttct tgtcactgtc
gcccattttc 4380gaagtgttct ccacccagat ttactcgcag gcgctcttga
acaacatgag cttcggtggt 4440gcgcgctaca tcgccacagg tcgtggtttc
gcgactagtc gcataccctt caacatcctc 4500tactcgcgtt tcgcgccgcc
aagcatctac atgggcatgc gtaacctgct gctcctgctg 4560tacgcgacga
tggccatttg gatcccgcac ctgatctact tctggttctc cgtcctctcc
4620ctctgcatcg cgccattcat gttcaatccg catcaattct cgtacgccga
cttcatcatc 4680gactaccggg agttcttgcg ctggatgtcg cgcggtaact
cgcgaacgaa ggcgagcagc 4740tggtacggat actgccgtct gtcgcgtacc
gcgattactg ggtacaagaa gaagaagctg 4800ggacacccgt cggagaagct
gtcgggcgac gtaccgcgtg cgccgtggag gaacgttatc 4860ttctcggaga
tcctgtggcc catcggcgcg tgcatcatct tcatcgtcgc gtacatgttc
4920gtcaagtcgt tccccgacga gcagggcaac gcgccgccga gcccgctggt
ccggattctg 4980ctcatcgcgg ttggccctac tgtgtggaac gcggcggtgc
tcataacgct gttcttcctg 5040tcgctcttcc tgggcccgat gatggatggc
tgggtcaagt tcggctcggt catggcggcc 5100cttgcgcatg gcctggcgct
tataggcatg ctcacgttct ttgagttctt cgtacgtcct 5160tcgcgttgtg
tcgtcaagtg ctctgctaac gccgtcttca gtggttcctt gagctctggg
5220atgcctcgca cgccgtgctc ggcgtcatcg ctatcattgc cgttcagcgc
gggatccaga 5280agatcctcat tgccgtcttc ctgacgcgtg agtacaagca
cgacgagacg aaccgcgcgt 5340ggtggacagg taaatggtat ggacgcgggc
tgggtacctc ggccatgtcc cagccggcgc 5400gcgagttcat cgtgaagatc
gtggagatgt cgttgtggac gtcggacttc ctgcttgcgc 5460acctgttgct
catcatcttg acggtgccgc tactgctgcc gttcttcaac tcaattcatt
5520cgacgatgct ttgtgagtgg tttgtagtcg ttggtcatgg atgatttctg
actcgcgtgc 5580agtctggttg cgcccttcga agcagattag gcaacctctg
ttctccacca agcagaagcg 5640gcaacggcga tggattgtga gttcctttga
ttgctctggg taccgacctt cgctcacctt 5700tcttaggtca tgaagtatac
cgtggtatat ctcgtggtgg tggctttcct cgtcgcgctc 5760atcgctctgc
gtacgttttc cctcgcgctc accctgtatt ttcactaacg tttcctccag
5820ccgccctctt ccgcgagagc atccacttca actgcgagat ctgccagagt
atatagtcat 5880ataacgacgt ctatcgtatc gccggacgag agccccgtcg
cctacacact gacatggaat 5940cgctgtgtat acaatcgatc ttctgaccgc
gtcgggggcg ttgccgtctt tctactatca 6000atttgcttgt gtatcaacat
ttcttctctc caagcctaca ttgacataga gtaatagccc 6060atgttcatac
aacaatcgca tagcattgca tataccat 6098101726PRTSchizophyllum commune
10Met Ser Gly Pro Gly Tyr Gly Arg Asn Pro Phe Asp Asn Pro Pro Pro 1
5 10 15 Asn Arg Gly Pro Tyr Gly Gln Gln Pro Gly Phe Pro Gly Pro Gly
Pro 20 25 30 Arg Pro Tyr Asp Ser Asp Ala Asp Met Ser Gln Thr Tyr
Gly Ser Thr 35 40 45 Thr Arg Leu Ala Gly Ser Ala Gly Tyr Ser Asp
Arg Asn Gly Ser Phe 50 55 60 Asp Gly Asp Arg Ser Tyr Ala Pro Ser
Ile Asp Ser Arg Ala Ser Val 65 70 75 80 Pro Ser Ile Ser Pro Phe Ala
Asp Pro Gly Ile Gly Ser Asn Glu Pro 85 90 95 Tyr Pro Ala Trp Ser
Val Glu Arg Gln Ile Pro Met Ser Thr Glu Glu 100 105 110 Ile Glu Asp
Ile Phe Leu Asp Leu Thr Gln Lys Phe Gly Phe Gln Arg 115 120 125 Asp
Ser Met Arg Asn Thr Phe Asp Phe Met Met His Leu Leu Asp Ser 130 135
140 Arg Ala Ser Arg Met Thr Pro Asn Gln Ala Leu Leu Thr Leu His Ala
145 150 155 160 Asp Tyr Ile Gly Gly Gln His Ala Asn Tyr Arg Lys Trp
Tyr Phe Ala 165 170 175 Ala Gln Leu Asn Leu Asp Asp Ala Val Gly Gln
Thr Asn Asn Pro Gly 180 185 190 Ile Gln Arg Leu Lys Thr Ile Lys Gly
Ala Thr Lys Thr Lys Ser Leu 195 200 205 Asp Ser Ala Leu Asn Arg Trp
Arg Asn Ala Met Asn Asn Met Ser Gln 210 215 220 Tyr Asp Arg Leu Arg
Gln Ile Ala Leu Tyr Leu Leu Cys Trp Gly Glu 225 230 235 240 Ala Gly
Asn Ile Arg Leu Ala Pro Glu Cys Leu Cys Phe Ile Phe Lys 245 250 255
Cys Ala Asp Asp Tyr Tyr Arg Ser Pro Glu Cys Gln Asn Arg Met Asp 260
265 270 Pro Val Pro Glu Gly Leu Tyr Leu Gln Thr Val Ile Lys Pro Leu
Tyr 275 280 285 Arg Phe Leu Arg Asp Gln Ala Tyr Glu Val Val Asp Gly
Lys
Gln Val 290 295 300 Lys Arg Glu Lys Asp His Asp Gln Ile Ile Gly Tyr
Asp Asp Val Asn 305 310 315 320 Gln Leu Phe Trp Tyr Pro Glu Gly Leu
Ala Lys Ile Val Met Ser Asp 325 330 335 Asn Thr Arg Leu Val Asp Val
Pro Pro Ala Gln Arg Phe Met Lys Phe 340 345 350 Ala Lys Ile Glu Trp
Asn Arg Val Phe Phe Lys Thr Tyr Phe Glu Lys 355 360 365 Arg Ser Thr
Ala His Leu Leu Val Asn Phe Asn Arg Ile Trp Ile Leu 370 375 380 His
Val Ser Met Tyr Phe Phe Tyr Thr Ala Phe Asn Ser Pro Arg Val 385 390
395 400 Tyr Ala Pro His Gly Lys Leu Asp Pro Ser Pro Glu Met Thr Trp
Ser 405 410 415 Ala Thr Ala Leu Gly Gly Ala Val Ser Thr Met Ile Met
Ile Leu Ala 420 425 430 Thr Ile Ala Glu Tyr Thr Tyr Ile Pro Thr Thr
Trp Asn Asn Ala Ser 435 440 445 His Leu Thr Thr Arg Leu Ile Phe Leu
Leu Val Ile Leu Ala Leu Thr 450 455 460 Ala Gly Pro Thr Phe Tyr Ile
Ala Met Ile Asp Gly Arg Thr Asp Ile 465 470 475 480 Gly Gln Val Pro
Leu Ile Val Ala Ile Val Gln Phe Phe Ile Ser Val 485 490 495 Val Ala
Thr Leu Ala Phe Ala Thr Ile Pro Ser Gly Arg Met Phe Gly 500 505 510
Asp Arg Val Ala Gly Lys Ser Arg Lys His Met Ala Ser Gln Thr Phe 515
520 525 Thr Ala Ser Tyr Pro Ser Met Lys Arg Ser Ser Arg Val Ala Ser
Ile 530 535 540 Met Leu Trp Leu Leu Val Phe Gly Cys Lys Tyr Val Glu
Ser Tyr Phe 545 550 555 560 Phe Leu Thr Ser Ser Phe Ser Ser Pro Ile
Ala Val Met Ala Arg Thr 565 570 575 Lys Val Gln Gly Cys Asn Asp Arg
Ile Phe Gly Ser Gln Leu Cys Thr 580 585 590 Asn Gln Val Pro Phe Ala
Leu Ala Ile Met Tyr Val Met Asp Leu Val 595 600 605 Leu Phe Phe Leu
Asp Thr Tyr Leu Trp Tyr Ile Ile Trp Leu Val Ile 610 615 620 Phe Ser
Met Val Arg Ala Phe Lys Leu Gly Ile Ser Ile Trp Thr Pro 625 630 635
640 Trp Ser Glu Ile Phe Thr Arg Met Pro Lys Arg Ile Tyr Ala Lys Leu
645 650 655 Leu Ala Thr Ala Glu Met Glu Val Lys Tyr Lys Pro Lys Val
Leu Val 660 665 670 Ser Gln Ile Trp Asn Ala Val Ile Ile Ser Met Tyr
Arg Glu His Leu 675 680 685 Leu Ser Ile Glu His Val Gln Arg Leu Leu
Tyr His Gln Val Asp Gly 690 695 700 Pro Asp Gly Arg Arg Thr Leu Arg
Ala Pro Pro Phe Phe Thr Ser Gln 705 710 715 720 Arg Thr Ala Lys Pro
Gly Leu Phe Phe Pro Pro Gly Gly Glu Ala Glu 725 730 735 Arg Arg Ile
Ser Phe Phe Ala Ser Ser Leu Thr Thr Ala Leu Pro Glu 740 745 750 Pro
Leu Pro Ile Asp Ala Met Pro Thr Phe Thr Val Leu Val Pro His 755 760
765 Tyr Ser Glu Lys Ile Leu Leu Ser Leu Arg Glu Ile Ile Arg Glu Glu
770 775 780 Asp Gln Asn Thr Arg Val Thr Leu Leu Glu Tyr Leu Lys Gln
Leu His 785 790 795 800 Pro Val Glu Trp Asp Asn Phe Val Lys Asp Thr
Lys Ile Leu Ala Glu 805 810 815 Glu Ser Gly Asp Val Gln Asp Glu Lys
Arg Ala Arg Thr Asp Asp Leu 820 825 830 Pro Phe Tyr Cys Ile Gly Phe
Lys Thr Ser Ser Pro Glu Tyr Thr Leu 835 840 845 Arg Thr Arg Ile Trp
Ala Ser Leu Arg Ala Gln Thr Leu Tyr Arg Thr 850 855 860 Val Ser Gly
Met Met Asn Tyr Ser Lys Ala Ile Lys Leu Leu Tyr Arg 865 870 875 880
Val Glu Asn Pro Asp Val Val His Ala Phe Gly Gly Asn Thr Glu Arg 885
890 895 Leu Glu Arg Glu Leu Glu Arg Met Ser Arg Arg Lys Phe Lys Phe
Val 900 905 910 Ile Ser Met Gln Arg Tyr Ser Lys Phe Asn Lys Glu Glu
Gln Glu Asn 915 920 925 Ala Glu Phe Leu Leu Arg Ala Tyr Pro Asp Leu
Gln Ile Ala Tyr Leu 930 935 940 Asp Glu Glu Pro Gly Pro Ser Lys Ser
Asp Glu Val Arg Leu Phe Ser 945 950 955 960 Thr Leu Ile Asp Gly His
Ser Glu Val Asp Glu Lys Thr Gly Arg Arg 965 970 975 Lys Pro Lys Phe
Arg Ile Glu Leu Pro Gly Asn Pro Ile Leu Gly Asp 980 985 990 Gly Lys
Ser Asp Asn Gln Asn His Ala Ile Val Phe Tyr Arg Gly Glu 995 1000
1005 Tyr Ile Gln Val Ile Asp Ala Asn Gln Asp Asn Tyr Leu Glu Glu
1010 1015 1020 Cys Leu Lys Ile Arg Asn Val Leu Gly Glu Phe Glu Glu
Tyr Ser 1025 1030 1035 Val Ser Ser Gln Ser Pro Tyr Ala Gln Trp Gly
His Lys Glu Phe 1040 1045 1050 Asn Lys Cys Pro Val Ala Ile Leu Gly
Ser Arg Glu Tyr Ile Phe 1055 1060 1065 Ser Glu Asn Ile Gly Ile Leu
Gly Asp Ile Ala Ala Gly Lys Glu 1070 1075 1080 Gln Thr Phe Gly Thr
Ile Thr Ala Arg Ala Leu Ala Trp Ile Gly 1085 1090 1095 Gly Lys Leu
His Tyr Gly His Pro Asp Phe Leu Asn Ala Thr Phe 1100 1105 1110 Met
Thr Thr Arg Gly Gly Val Ser Lys Ala Gln Lys Gly Leu His 1115 1120
1125 Leu Asn Glu Asp Ile Phe Ala Gly Met Thr Ala Val Ser Arg Gly
1130 1135 1140 Gly Arg Ile Lys His Met Glu Tyr Tyr Gln Cys Gly Lys
Gly Arg 1145 1150 1155 Asp Leu Gly Phe Gly Thr Ile Leu Asn Phe Gln
Thr Lys Ile Gly 1160 1165 1170 Thr Gly Met Gly Glu Gln Leu Leu Ser
Arg Glu Tyr Tyr Tyr Leu 1175 1180 1185 Gly Thr Gln Leu Pro Ile Asp
Arg Phe Leu Thr Phe Tyr Tyr Ala 1190 1195 1200 His Ala Gly Phe His
Val Asn Asn Ile Leu Val Ile Tyr Ser Ile 1205 1210 1215 Gln Val Phe
Met Val Thr Leu Leu Tyr Leu Gly Thr Leu Asn Lys 1220 1225 1230 Gln
Leu Phe Ile Cys Lys Val Asn Ser Asn Gly Gln Val Leu Ser 1235 1240
1245 Gly Gln Ala Gly Cys Tyr Asn Leu Ile Pro Val Phe Glu Trp Ile
1250 1255 1260 Arg Arg Ser Ile Ile Ser Ile Phe Leu Val Phe Phe Ile
Ala Phe 1265 1270 1275 Leu Pro Leu Phe Leu Gln Glu Leu Cys Glu Arg
Gly Thr Gly Lys 1280 1285 1290 Ala Leu Leu Arg Leu Gly Lys His Phe
Leu Ser Leu Ser Pro Ile 1295 1300 1305 Phe Glu Val Phe Ser Thr Gln
Ile Tyr Ser Gln Ala Leu Leu Asn 1310 1315 1320 Asn Met Ser Phe Gly
Gly Ala Arg Tyr Ile Ala Thr Gly Arg Gly 1325 1330 1335 Phe Ala Thr
Ser Arg Ile Pro Phe Asn Ile Leu Tyr Ser Arg Phe 1340 1345 1350 Ala
Pro Pro Ser Ile Tyr Met Gly Met Arg Asn Leu Leu Leu Leu 1355 1360
1365 Leu Tyr Ala Thr Met Ala Ile Trp Ile Pro His Leu Ile Tyr Phe
1370 1375 1380 Trp Phe Ser Val Leu Ser Leu Cys Ile Ala Pro Phe Met
Phe Asn 1385 1390 1395 Pro His Gln Phe Ser Tyr Ala Asp Phe Ile Ile
Asp Tyr Arg Glu 1400 1405 1410 Phe Leu Arg Trp Met Ser Arg Gly Asn
Ser Arg Thr Lys Ala Ser 1415 1420 1425 Ser Trp Tyr Gly Tyr Cys Arg
Leu Ser Arg Thr Ala Ile Thr Gly 1430 1435 1440 Tyr Lys Lys Lys Lys
Leu Gly His Pro Ser Glu Lys Leu Ser Gly 1445 1450 1455 Asp Val Pro
Arg Ala Pro Trp Arg Asn Val Ile Phe Ser Glu Ile 1460 1465 1470 Leu
Trp Pro Ile Gly Ala Cys Ile Ile Phe Ile Val Ala Tyr Met 1475 1480
1485 Phe Val Lys Ser Phe Pro Asp Glu Gln Gly Asn Ala Pro Pro Ser
1490 1495 1500 Pro Leu Val Arg Ile Leu Leu Ile Ala Val Gly Pro Thr
Val Trp 1505 1510 1515 Asn Ala Ala Val Leu Ile Thr Leu Phe Phe Leu
Ser Leu Phe Leu 1520 1525 1530 Gly Pro Met Met Asp Gly Trp Val Lys
Phe Gly Ser Val Met Ala 1535 1540 1545 Ala Leu Ala His Gly Leu Ala
Leu Ile Gly Met Leu Thr Phe Phe 1550 1555 1560 Glu Phe Phe Trp Phe
Leu Glu Leu Trp Asp Ala Ser His Ala Val 1565 1570 1575 Leu Gly Val
Ile Ala Ile Ile Ala Val Gln Arg Gly Ile Gln Lys 1580 1585 1590 Ile
Leu Ile Ala Val Phe Leu Thr Arg Lys Trp Tyr Gly Arg Gly 1595 1600
1605 Leu Gly Thr Ser Ala Met Ser Gln Pro Ala Arg Glu Phe Ile Val
1610 1615 1620 Lys Ile Val Glu Met Ser Leu Trp Thr Ser Asp Phe Leu
Leu Ala 1625 1630 1635 His Leu Leu Leu Ile Ile Leu Thr Val Pro Leu
Leu Leu Pro Phe 1640 1645 1650 Phe Asn Ser Ile His Ser Thr Met Leu
Phe Trp Leu Arg Pro Ser 1655 1660 1665 Lys Gln Ile Arg Gln Pro Leu
Phe Ser Thr Lys Gln Lys Arg Gln 1670 1675 1680 Arg Arg Trp Ile Val
Met Lys Tyr Thr Val Val Tyr Leu Val Val 1685 1690 1695 Val Ala Phe
Leu Val Ala Leu Ile Ala Leu Pro Ala Leu Phe Arg 1700 1705 1710 Glu
Ser Ile His Phe Asn Cys Glu Ile Cys Gln Ser Ile 1715 1720 1725 11
5771DNASchizophyllum commune 11ctgtccaagg aggagatcga ggacatcttc
ctcgatttga cgcagaagtt tggctttcag 60cgggattcca tgcggaatat ggtacgtggc
gtgtgcccat gtgcggcgtt ctgaggccta 120acgttttccg ccagttcgac
ttcaccatgc agctgcttga cagccgagcg tctcgtatga 180cccccaacca
ggcgctcctc accctccacg ccgactacat tggtggccag catgcgaact
240accggaagtg gtacttcgcg gcgcagctcg accttgacga cgccgtggga
caaactcaga 300atccgggtct caaccgcctc aagtccactc gcggatcggg
caagcgacca cgccatgaaa 360agtcgctgaa cacggcattg gagcgctggc
ggcaagccat gaacaacatg tcgcagtatg 420accgcttacg ccagatcgcg
ctctacctgc tctgctgggg cgaagcggcg caagtgcgat 480tcatgcccga
gtgcttgtgc ttcatcttca agtgcgccga cgactactat cgttcgccgg
540agtgccagaa caggatggag ccggtaccgg agggtctcta cctgaggacg
gtcgtaaagc 600cgctctacag atttgtccgg gatcaaggct atgaggtggt
ggagggaaaa ttcgtacggc 660gggaacggga tcacgaccaa atcattggtt
acgatgacgt gaatcagctg ttctggtacc 720cggagggaat tgcccgtatc
gtcctgtcgg acaaggtaag cacctctgtg catcttctgt 780gacatacagg
gctaattgtc gagcagagtc gtctagtcga cctcccccca gcacagcgct
840tcatgaagtt cgaccgtatc gagtggaatc gcgtcttctt caagacgttt
tacgagactc 900gatccttcac gcatcttttg gtcgacttca accgtatctg
ggtcgtgcac atcgctctct 960acttcttcta cactgcatac aactccccca
cgatctacgc catcaacggc aacacaccga 1020cgtctctggc ttggagcgcg
actgcgctcg gcggtgcggt agcgacaggt atcatgatcc 1080tcgccacgat
cgccgagttc tcgcacatcc ccacgacatg gaacaacacc tcgcatctga
1140ctcgccgcct cgccttcctc ctcgtcacgc tcggcctcac atgtggtccg
acgttctacg 1200tcgcgattgc agagagcaac gggagcggcg gctctttggc
cttgattctc ggtatcgtcc 1260agttcttcat ctccgtcgtg gcaactgcgc
tcttcactat catgccttct ggtcgtatgt 1320tcggcgaccg tgtcgcaggc
aagagtcgca agtatctcgc cagccagacg ttcacggcca 1380gctacccgtc
gttgcccaag caccagcggt tcgcctcact cctgatgtgg ttcctcatct
1440tcgggtgcaa gttgacggag agttacttct ttctgacgct gtccttccgc
gaccctatcc 1500gcgtcatggt cggcatgaag atccagaact gcgaggacaa
gattttcggc agcggccttt 1560gcaggaatca cgcagcattc accctcacga
tcatgtacat catggacctc gtcttgttct 1620tcctcgacac cttcctttgg
tatgtcatct ggaactcggt tttcagtatc gcacgctctt 1680tcgtactcgg
cctttcgatc tggacaccgt ggagagacat cttccagcgt ctgccgaagc
1740ggatctacgc gaagcttctg gcgactggcg acatggaggt caagtacaag
cccaaggtat 1800gcgttgagct cgccgtaaat ccacttaagg ctaacacgtt
cgcaggtctt ggtctcgcaa 1860atctggaacg ccatcatcat ctccatgtac
cgcgagcact tgctctctat tgagcacgtc 1920cagaagctcc tgtaccacca
agtggacact ggcgaagccg gcaagcggag tcttcgcgcg 1980cctccgttct
tcgtcgcgca gggcagcagc ggtggctcgg gcgagttctt cccgcctggc
2040agcgaggccg agcgtcgtat ctctttcttc gcgcagtcgc tttctacgga
gattcctcag 2100cccatcccgg tcgacgccat gccgacgttc acggtgctta
cgcctcacta cagcgagaag 2160gtacatgctc cccttgtagc catatgacat
cagctgactg tcgtgcacag atccttctct 2220ctctccgtga aattatccgc
gaggaggacc agaacactcg cgttacgttg ctcgagtacc 2280tgaagcagct
gcatccggtc gagtgggaga atttcgtcaa ggacactaaa attttggccg
2340aggagtccgc tatgtttaac ggtccgagtc ctttcggcaa cgacgagaag
ggtcagtcca 2400agatggacga tctaccgttc tactgcatcg gtttcaagag
cgccgcgccc gagtacaccc 2460tccgcacccg tatctgggcg tccctgcgcg
cgcagacgct gtaccgcacg gtctccggca 2520tgatgaacta tgcgaaggcg
atcaagctgc tctaccgcgt tgagaacccg gaggtcgtac 2580aacagttcgg
cggcaacacg gacaagctcg agcgcgagtt ggagcggatg gcgcgacgga
2640agttcaagtt cctcgtgtcc atgcagcgct actcgaagtt caacaaggag
gagcacgaga 2700acgccgagtt cttgctccgc gcgtacccgg acttgcagat
cgcgtacctc gaggaagagc 2760cccctcgcaa ggagggcggc gatccacgca
tcttctctgc cctcgtcgac ggccacagcg 2820acatcatccc ggagaccggc
aagcggcgcc ccaagttccg tatcgagctg cccggtaacc 2880ccattctcgg
tgacggtaaa tccgacaatc agaaccacgc tatcgtcttc taccgcggcg
2940agtacctcca gcttatcgac gccaaccagg acaactacct cgaggagtgc
ttgaagatcc 3000gtaacgtgct cgccgagttt gaggagtacg acgtctccag
ccagagcccg tacgcgcagt 3060ggagtgtcaa ggagttcaag cgctctccgg
tcgccatcgt cggtgcacgc gagtacatct 3120tctcagagca catcggtatc
ctcggtgatc tggcggctgg caaggaacag acgttcggta 3180cgctcacggc
acgcaacaac gccttccttg gcggcaagct gcactacggt caccccgatt
3240tcctcaacgc cctctacatg aacacgcgcg gtggtgtctc caaggcgcag
aagggtctcc 3300atctcaacga ggatatctac gccggtatga acgcggtcgg
tcgcggtgga cgcattaagc 3360acagcgagta ctatcagtgc ggcaagggtc
gtgacctcgg tttcggcacc atcttgaact 3420tccagaccaa gatcggtacg
ggtatgggcg agcagatcct ctcgcgcgag tactactatc 3480tcggaacaca
actgcccatc gatcgcttcc tcacgttcta ctacgcgcac ccgggtttcc
3540agatcaacaa catgctggtc atcctctccg tgcaggtctt catcgttacc
agtacgttca 3600atgcatattg ttagcctgac aacgtctgac gaatttccag
tggtcttcct cggtaccttg 3660aagtcttcgg tcacgatctg caagtacacg
tccagcggtc agtacatcgg tggtcaatcc 3720ggttgctaca acctcgtccc
ggtcttccag tggatcgagc gctgcatcat cagcatcttc 3780ttggtgttca
tgatcgcttt catgccgctc ttcctgcaag gtaagagctt gtcaacctgc
3840tcaaggggct tgcgctgatc atcatctcag aactcgtcga gcgcggtacc
tggagtgcca 3900tctggcgtct gctcaagcag tttatgtcgc tgtcgcctgt
cttcgaggtg ttctccaccc 3960agattcagac gcactccgtg ttgagcaact
tgacgttcgg tggtgcgcgt tacatcgcta 4020ccggtcgtgg gttcgccacc
agtcgtatca gcttcagcat cttgttctcg cgtttcgcag 4080gcccgagtat
ctacctcggc atgcgcacgc tcattatgct gctctacgtg acgttgacga
4140tctggacgcc atgggtcatt tacttctggg tttccattct ctcgctctgc
atcgcgccgt 4200tcttgttcaa cccgcatcaa ttcgtattct cggacttcct
catcgactac aggtacgtcg 4260gacgagcgct gttccgcgac gtaagctgac
cggttataca gggaatacct gcggtggatg 4320tcgcgtggca actcgcgctc
gcacaacaac tcctggattg ggtactgccg gttgtcccgc 4380acgatgatca
ctgggtacaa gaagaagaag ctgggccacc cgtcggagaa gctttccggc
4440gacgttcctc gtgcaggctg gcgcgccgtc ttgttctcgg agatcatctt
cccggcgtgc 4500atggccatcc tcttcatcat cgcgtacatg ttcgtcaagt
cgttccctct cgacggcaag 4560cagcctccct ccggcctcgt tcgcatcgcc
gtcgtgtcta tcggccccat cgtgtggaac 4620gccgccatcc tgttgacgct
cttccttgtg tcgttgttcc tcggccccat gctcgacccg 4680gtcttccccc
tcttcggttc cgttatggcc ttcatcgcgc atttccttgg cacaatcgga
4740atgattgggt tcttcgagtt cctggtatgt gcccatacct ttcattcgac
ttcaactatc 4800taacagattc atagtggttc ctcgagtcct gggaggcgtc
gcatgccgtg ctgggtctca 4860tcgccgtcat ctccatccag cgcgccattc
acaagatcct tatcgccgtt ttcctcagtc 4920gcgagttcaa gcacgacgag
acgaacaggg cctggtggac tggtcgctgg tatggccgtg 4980gcctcggcac
gcacgccatg tcgcagccgg cgcgtgagtt cgtcgtcaag atcatcgagt
5040tgtcgctttg gagctcggat ctcatactcg gccacatcct gctgttcatg
cttactccgg 5100ccgtcctcat cccgtacttc gaccgtttgc acgccatgat
gctctgtacg tcgtgtctca 5160ttgtctgtgt tggtcatact cttaccctct
cttagtctgg ctgcgtccct cgaagcaaat 5220ccgcgcgcct ctgtactcga
tcaagcagaa gaggcaaaga cgctggattg tcagtgttca 5280gtgccttatt
ctatcagctc ttactaacgt cttcatagat catgaagtac ggtactgtat
5340acgttaccgt catcgcgatc ttcgtcgcgc tcatcgcgct tcgtgagttt
ccttgctatt 5400tttcgtacct gagcgtcgct gacccctttc
ccagccctcg tattccgaca cactctaaag 5460gtcgagtgct ccctttgcga
cagcttgtaa tatcggactc gtatatatct agacttctcc 5520gcaccatgtg
tagctgacgc ttgggtatac ttcgcggtgc cgagctaatt gtcgacggac
5580attctccatc gttgagtgca gcgacgtcgg gtggtttacg acacggacac
ttttcattgt 5640accctctacg aatgcaagaa ctctcttacg accagtacct
atgtgctaag ccgtcgcctg 5700ttcaggatca tacatacata cgtttctaga
taccttacag ttaggcctat tcagggagag 5760tctgcataaa a
5771121783PRTSchizophyllum commune 12Met Pro Arg Pro Gly Gly Thr
Ser Ala Glu Gly Gly Tyr Ala Ser Ser 1 5 10 15 Pro Ser Met Glu Thr
Thr Pro Ser Asp Pro Phe Gly Thr Ala Asn Gly 20 25 30 Ala Pro Arg
Arg Tyr Tyr Asp Asn Asp Ser Glu Glu Tyr Gly Pro Gly 35 40 45 Arg
Arg Asp Thr Tyr Ala Ser Asp Ser Ser Asn Gln Gly Leu Thr Asp 50 55
60 Pro Gly Tyr Tyr Asp Gln Asn Gly Ala Tyr Asp Pro Tyr Pro Thr Gly
65 70 75 80 Asp Thr Asp Ser Asp Gly Asp Val Tyr Gly Gln Arg Tyr Gly
Pro Ser 85 90 95 Ala Glu Ser Leu Gly Thr His Lys Phe Gly His Ser
Asp Ser Ser Thr 100 105 110 Pro Thr Phe Val Asp Tyr Ser Ala Ser Ser
Gly Gly Arg Asp Ser Tyr 115 120 125 Pro Ala Trp Thr Ala Glu Arg Asn
Ile Pro Leu Ser Lys Glu Glu Ile 130 135 140 Glu Asp Ile Phe Leu Asp
Leu Thr Gln Lys Phe Gly Phe Gln Arg Asp 145 150 155 160 Ser Met Arg
Asn Met Phe Asp Phe Thr Met Gln Leu Leu Asp Ser Arg 165 170 175 Ala
Ser Arg Met Thr Pro Asn Gln Ala Leu Leu Thr Leu His Ala Asp 180 185
190 Tyr Ile Gly Gly Gln His Ala Asn Tyr Arg Lys Trp Tyr Phe Ala Ala
195 200 205 Gln Leu Asp Leu Asp Asp Ala Val Gly Gln Thr Gln Asn Pro
Gly Leu 210 215 220 Asn Arg Leu Lys Ser Thr Arg Gly Ser Gly Lys Arg
Pro Arg His Glu 225 230 235 240 Lys Ser Leu Asn Thr Ala Leu Glu Arg
Trp Arg Gln Ala Met Asn Asn 245 250 255 Met Ser Gln Tyr Asp Arg Leu
Arg Gln Ile Ala Leu Tyr Leu Leu Cys 260 265 270 Trp Gly Glu Ala Ala
Gln Val Arg Phe Met Pro Glu Cys Leu Cys Phe 275 280 285 Ile Phe Lys
Cys Ala Asp Asp Tyr Tyr Arg Ser Pro Glu Cys Gln Asn 290 295 300 Arg
Met Glu Pro Val Pro Glu Gly Leu Tyr Leu Arg Thr Val Val Lys 305 310
315 320 Pro Leu Tyr Arg Phe Val Arg Asp Gln Gly Tyr Glu Val Val Glu
Gly 325 330 335 Lys Phe Val Arg Arg Glu Arg Asp His Asp Gln Ile Ile
Gly Tyr Asp 340 345 350 Asp Val Asn Gln Leu Phe Trp Tyr Pro Glu Gly
Ile Ala Arg Ile Val 355 360 365 Leu Ser Asp Lys Ser Arg Leu Val Asp
Leu Pro Pro Ala Gln Arg Phe 370 375 380 Met Lys Phe Asp Arg Ile Glu
Trp Asn Arg Val Phe Phe Lys Thr Phe 385 390 395 400 Tyr Glu Thr Arg
Ser Phe Thr His Leu Leu Val Asp Phe Asn Arg Ile 405 410 415 Trp Val
Val His Ile Ala Leu Tyr Phe Phe Tyr Thr Ala Tyr Asn Ser 420 425 430
Pro Thr Ile Tyr Ala Ile Asn Gly Asn Thr Pro Thr Ser Leu Ala Trp 435
440 445 Ser Ala Thr Ala Leu Gly Gly Ala Val Ala Thr Gly Ile Met Ile
Leu 450 455 460 Ala Thr Ile Ala Glu Phe Ser His Ile Pro Thr Thr Trp
Asn Asn Thr 465 470 475 480 Ser His Leu Thr Arg Arg Leu Ala Phe Leu
Leu Val Thr Leu Gly Leu 485 490 495 Thr Cys Gly Pro Thr Phe Tyr Val
Ala Ile Ala Glu Ser Asn Gly Ser 500 505 510 Gly Gly Ser Leu Ala Leu
Ile Leu Gly Ile Val Gln Phe Phe Ile Ser 515 520 525 Val Val Ala Thr
Ala Leu Phe Thr Ile Met Pro Ser Gly Arg Met Phe 530 535 540 Gly Asp
Arg Val Ala Gly Lys Ser Arg Lys Tyr Leu Ala Ser Gln Thr 545 550 555
560 Phe Thr Ala Ser Tyr Pro Ser Leu Pro Lys His Gln Arg Phe Ala Ser
565 570 575 Leu Leu Met Trp Phe Leu Ile Phe Gly Cys Lys Leu Thr Glu
Ser Tyr 580 585 590 Phe Phe Leu Thr Leu Ser Phe Arg Asp Pro Ile Arg
Val Met Val Gly 595 600 605 Met Lys Ile Gln Asn Cys Glu Asp Lys Ile
Phe Gly Ser Gly Leu Cys 610 615 620 Arg Asn His Ala Ala Phe Thr Leu
Thr Ile Met Tyr Ile Met Asp Leu 625 630 635 640 Val Leu Phe Phe Leu
Asp Thr Phe Leu Trp Tyr Val Ile Trp Asn Ser 645 650 655 Val Phe Ser
Ile Ala Arg Ser Phe Val Leu Gly Leu Ser Ile Trp Thr 660 665 670 Pro
Trp Arg Asp Ile Phe Gln Arg Leu Pro Lys Arg Ile Tyr Ala Lys 675 680
685 Leu Leu Ala Thr Gly Asp Met Glu Val Lys Tyr Lys Pro Lys Val Leu
690 695 700 Val Ser Gln Ile Trp Asn Ala Ile Ile Ile Ser Met Tyr Arg
Glu His 705 710 715 720 Leu Leu Ser Ile Glu His Val Gln Lys Leu Leu
Tyr His Gln Val Asp 725 730 735 Thr Gly Glu Ala Gly Lys Arg Ser Leu
Arg Ala Pro Pro Phe Phe Val 740 745 750 Ala Gln Gly Ser Ser Gly Gly
Ser Gly Glu Phe Phe Pro Pro Gly Ser 755 760 765 Glu Ala Glu Arg Arg
Ile Ser Phe Phe Ala Gln Ser Leu Ser Thr Glu 770 775 780 Ile Pro Gln
Pro Ile Pro Val Asp Ala Met Pro Thr Phe Thr Val Leu 785 790 795 800
Thr Pro His Tyr Ser Glu Lys Ile Leu Leu Ser Leu Arg Glu Ile Ile 805
810 815 Arg Glu Glu Asp Gln Asn Thr Arg Val Thr Leu Leu Glu Tyr Leu
Lys 820 825 830 Gln Leu His Pro Val Glu Trp Glu Asn Phe Val Lys Asp
Thr Lys Ile 835 840 845 Leu Ala Glu Glu Ser Ala Met Phe Asn Gly Pro
Ser Pro Phe Gly Asn 850 855 860 Asp Glu Lys Gly Gln Ser Lys Met Asp
Asp Leu Pro Phe Tyr Cys Ile 865 870 875 880 Gly Phe Lys Ser Ala Ala
Pro Glu Tyr Thr Leu Arg Thr Arg Ile Trp 885 890 895 Ala Ser Leu Arg
Ala Gln Thr Leu Tyr Arg Thr Val Ser Gly Met Met 900 905 910 Asn Tyr
Ala Lys Ala Ile Lys Leu Leu Tyr Arg Val Glu Asn Pro Glu 915 920 925
Val Val Gln Gln Phe Gly Gly Asn Thr Asp Lys Leu Glu Arg Glu Leu 930
935 940 Glu Arg Met Ala Arg Arg Lys Phe Lys Phe Leu Val Ser Met Gln
Arg 945 950 955 960 Tyr Ser Lys Phe Asn Lys Glu Glu His Glu Asn Ala
Glu Phe Leu Leu 965 970 975 Arg Ala Tyr Pro Asp Leu Gln Ile Ala Tyr
Leu Glu Glu Glu Pro Pro 980 985 990 Arg Lys Glu Gly Gly Asp Pro Arg
Ile Phe Ser Ala Leu Val Asp Gly 995 1000 1005 His Ser Asp Ile Ile
Pro Glu Thr Gly Lys Arg Arg Pro Lys Phe 1010 1015 1020 Arg Ile Glu
Leu Pro Gly Asn Pro Ile Leu Gly Asp Gly Lys Ser 1025 1030 1035 Asp
Asn Gln Asn His Ala Ile Val Phe Tyr Arg Gly Glu Tyr Leu 1040 1045
1050 Gln Leu Ile Asp Ala Asn Gln Asp Asn Tyr Leu Glu Glu Cys Leu
1055 1060 1065 Lys Ile Arg Asn Val Leu Ala Glu Phe Glu Glu Tyr Asp
Val Ser 1070 1075 1080 Ser Gln Ser Pro Tyr Ala Gln Trp Ser Val Lys
Glu Phe Lys Arg 1085 1090 1095 Ser Pro Val Ala Ile Val Gly Ala Arg
Glu Tyr Ile Phe Ser Glu 1100 1105 1110 His Ile Gly Ile Leu Gly Asp
Leu Ala Ala Gly Lys Glu Gln Thr 1115 1120 1125 Phe Gly Thr Leu Thr
Ala Arg Asn Asn Ala Phe Leu Gly Gly Lys 1130 1135 1140 Leu His Tyr
Gly His Pro Asp Phe Leu Asn Ala Leu Tyr Met Asn 1145 1150 1155 Thr
Arg Gly Gly Val Ser Lys Ala Gln Lys Gly Leu His Leu Asn 1160 1165
1170 Glu Asp Ile Tyr Ala Gly Met Asn Ala Val Gly Arg Gly Gly Arg
1175 1180 1185 Ile Lys His Ser Glu Tyr Tyr Gln Cys Gly Lys Gly Arg
Asp Leu 1190 1195 1200 Gly Phe Gly Thr Ile Leu Asn Phe Gln Thr Lys
Ile Gly Thr Gly 1205 1210 1215 Met Gly Glu Gln Ile Leu Ser Arg Glu
Tyr Tyr Tyr Leu Gly Thr 1220 1225 1230 Gln Leu Pro Ile Asp Arg Phe
Leu Thr Phe Tyr Tyr Ala His Pro 1235 1240 1245 Gly Phe Gln Ile Asn
Asn Met Leu Val Ile Leu Ser Val Gln Val 1250 1255 1260 Phe Ile Val
Thr Met Val Phe Leu Gly Thr Leu Lys Ser Ser Val 1265 1270 1275 Thr
Ile Cys Lys Tyr Thr Ser Ser Gly Gln Tyr Ile Gly Gly Gln 1280 1285
1290 Ser Gly Cys Tyr Asn Leu Val Pro Val Phe Gln Trp Ile Glu Arg
1295 1300 1305 Cys Ile Ile Ser Ile Phe Leu Val Phe Met Ile Ala Phe
Met Pro 1310 1315 1320 Leu Phe Leu Gln Glu Leu Val Glu Arg Gly Thr
Trp Ser Ala Ile 1325 1330 1335 Trp Arg Leu Leu Lys Gln Phe Met Ser
Leu Ser Pro Val Phe Glu 1340 1345 1350 Val Phe Ser Thr Gln Ile Gln
Thr His Ser Val Leu Ser Asn Leu 1355 1360 1365 Thr Phe Gly Gly Ala
Arg Tyr Ile Ala Thr Gly Arg Gly Phe Ala 1370 1375 1380 Thr Ser Arg
Ile Ser Phe Ser Ile Leu Phe Ser Arg Phe Ala Gly 1385 1390 1395 Pro
Ser Ile Tyr Leu Gly Met Arg Thr Leu Ile Met Leu Leu Tyr 1400 1405
1410 Val Thr Leu Thr Ile Trp Thr Pro Trp Val Ile Tyr Phe Trp Val
1415 1420 1425 Ser Ile Leu Ser Leu Cys Ile Ala Pro Phe Leu Phe Asn
Pro His 1430 1435 1440 Gln Phe Val Phe Ser Asp Phe Leu Ile Asp Tyr
Arg Glu Tyr Leu 1445 1450 1455 Arg Trp Met Ser Arg Gly Asn Ser Arg
Ser His Asn Asn Ser Trp 1460 1465 1470 Ile Gly Tyr Cys Arg Leu Ser
Arg Thr Met Ile Thr Gly Tyr Lys 1475 1480 1485 Lys Lys Lys Leu Gly
His Pro Ser Glu Lys Leu Ser Gly Asp Val 1490 1495 1500 Pro Arg Ala
Gly Trp Arg Ala Val Leu Phe Ser Glu Ile Ile Phe 1505 1510 1515 Pro
Ala Cys Met Ala Ile Leu Phe Ile Ile Ala Tyr Met Phe Val 1520 1525
1530 Lys Ser Phe Pro Leu Asp Gly Lys Gln Pro Pro Ser Gly Leu Val
1535 1540 1545 Arg Ile Ala Val Val Ser Ile Gly Pro Ile Val Trp Asn
Ala Ala 1550 1555 1560 Ile Leu Leu Thr Leu Phe Leu Val Ser Leu Phe
Leu Gly Pro Met 1565 1570 1575 Leu Asp Pro Val Phe Pro Leu Phe Gly
Ser Val Met Ala Phe Ile 1580 1585 1590 Ala His Phe Leu Gly Thr Ile
Gly Met Ile Gly Phe Phe Glu Phe 1595 1600 1605 Leu Trp Phe Leu Glu
Ser Trp Glu Ala Ser His Ala Val Leu Gly 1610 1615 1620 Leu Ile Ala
Val Ile Ser Ile Gln Arg Ala Ile His Lys Ile Leu 1625 1630 1635 Ile
Ala Val Phe Leu Ser Arg Glu Phe Lys His Asp Glu Thr Asn 1640 1645
1650 Arg Ala Trp Trp Thr Gly Arg Trp Tyr Gly Arg Gly Leu Gly Thr
1655 1660 1665 His Ala Met Ser Gln Pro Ala Arg Glu Phe Val Val Lys
Ile Ile 1670 1675 1680 Glu Leu Ser Leu Trp Ser Ser Asp Leu Ile Leu
Gly His Ile Leu 1685 1690 1695 Leu Phe Met Leu Thr Pro Ala Val Leu
Ile Pro Tyr Phe Asp Arg 1700 1705 1710 Leu His Ala Met Met Leu Phe
Trp Leu Arg Pro Ser Lys Gln Ile 1715 1720 1725 Arg Ala Pro Leu Tyr
Ser Ile Lys Gln Lys Arg Gln Arg Arg Trp 1730 1735 1740 Ile Ile Met
Lys Tyr Gly Thr Val Tyr Val Thr Val Ile Ala Ile 1745 1750 1755 Phe
Val Ala Leu Ile Ala Leu Pro Leu Val Phe Arg His Thr Leu 1760 1765
1770 Lys Val Glu Cys Ser Leu Cys Asp Ser Leu 1775 1780 13
5223DNASchizophyllum commune 13atgtccggtc caggatatgg caggaatcca
ttcgacaatc ccccgcccaa cagaggtccc 60tatggccagc agccaggttt cccggggccc
ggccctcggc cttacgactc ggacgcggac 120atgagccaga cctatggcag
cacaaccagg ctcgccggca gtgccggtta cagcgacaga 180aacggcagct
tcgacggcga ccgctcctac gcgccctcaa ttgactcgcg cgccagcgtg
240cccagcatat cgcccttcgc agacccgggt atcggctcta atgagccgta
tcccgcttgg 300tcggtcgaac gccagatccc catgtccacg gaggagattg
aggatatctt cctcgacctc 360acccaaaagt ttggcttcca gcgcgactcc
atgcggaata cgttcgactt catgatgcac 420ctccttgatt cccgtgcctc
gcgcatgacg cccaaccaag ctctgctcac gcttcacgcc 480gactacattg
gtggccagca cgccaactat aggaagtggt atttcgccgc tcagctcaac
540ctcgatgacg cggtcgggca aaccaataac cccggtatcc agcgcttgaa
gaccatcaag 600ggcgctacga agaccaagtc gctcgacagc gcactcaacc
gctggcgcaa tgcgatgaac 660aacatgagcc agtacgatcg cctccggcaa
attgcgctct atctcctctg ctggggagaa 720gcaggcaaca tccgtctggc
gcccgagtgc ttgtgcttca tcttcaagtg cgcggacgac 780tactacagaa
gtcccgagtg tcagaaccgg atggaccccg tgccggaagg gctgtacctc
840cagacggtca tcaagccgct ctatcgcttc ctacgtgatc aggcgtacga
agtcgttgat 900gggaagcaag tgaagcgcga gaaggaccac gaccagatta
tcggttatga cgacgtcaac 960cagttattct ggtatccgga aggtttggct
aagatcgtca tgtcggacaa cacacgactt 1020gtagatgtac ctccggcgca
gcggttcatg aagttcgcca agatcgagtg gaaccgcgtc 1080ttcttcaaga
cgtactttga gaagcgctct actgcccatc tcctggtcaa cttcaaccgt
1140atatggatcc tccacgtctc gatgtacttc ttctacacgg cattcaactc
tccacgagtc 1200tacgcgccgc acggcaaact cgacccctcc cctgagatga
cctggtccgc gactgccctt 1260ggaggcgctg tgtccaccat gatcatgatc
cttgccacta tcgcggagta cacctacatc 1320cccacgacat ggaacaatgc
gtcgcacctc accacgcggc tcattttcct cctggtcatc 1380ctcgcgctca
ctgctggacc aacattctat atcgccatga tagacggacg cacggacatc
1440ggccaagtac cactcatcgt ggccatagtg cagttcttca tctccgtcgt
cgccaccctc 1500gctttcgcta ccatcccttc tggtcgcatg ttcggcgacc
gtgtggctgg caagtcaaga 1560aagcacatgg catcgcagac gttcacagcg
tcgtacccgt ccatgaagcg gtcatctcgc 1620gtagcgagta tcatgctgtg
gcttttggtc tttggctgca aatacgtcga gtcttacttc 1680ttcttgacgt
cctccttctc cagcccgatc gcggtcatgg cgcgtacgaa ggtacagggc
1740tgcaacgacc gtatcttcgg cagccagctg tgcacgaatc aggtcccgtt
cgcgctggca 1800atcatgtacg tgatggacct ggtactgttc ttcctggaca
cgtacctgtg gtacatcatc 1860tggctggtga tcttctcgat ggtgcgcgcg
ttcaagcttg gtatctcgat ctggacgccc 1920tggagcgaga tcttcacccg
catgccgaag cgtatctacg cgaagctgct ggcgacggcc 1980gagatggagg
tcaagtataa gcccaaggtg ctcgtctcgc aaatctggaa cgcggtcatc
2040atctccatgt accgggagca tctcttgtcc atcgagcacg tccagcgcct
gctataccac 2100caggttgatg gtccagacgg tcgccgcacc ctcagggcac
cgccgttctt caccagccag 2160cgaactgcga agccaggcct gttcttccct
cctggtggcg aggctgagcg ccgtatctcg 2220ttctttgcct catcgctgac
gaccgcgctc cctgagcctc tgccgatcga cgccatgccc 2280accttcaccg
tgctcgttcc ccattactcg gagaagattc tgctcagtct gcgcgagatt
2340attcgcgagg aggaccagaa cacccgcgtc accttgctgg agtacctcaa
gcagctccac 2400cctgtcgaat gggacaactt cgtcaaggac accaagatct
tggcggaaga gtcgggcgac 2460gtccaggacg agaagcgcgc gcgcacggac
gacttgccgt tctactgcat cgggttcaag 2520acctcgtcac cagagtacac
cctgcgtacg cgtatctggg cttcactgcg cgcacagacg 2580ctgtaccgca
cggtctccgg tatgatgaac tactccaagg cgatcaagct cctctatcgc
2640gtcgagaacc cggatgtcgt tcatgccttc ggtgggaaca cggaacgtct
tgaacgcgag 2700cttgagcgca
tgtctcgccg caagttcaag ttcgtcatct cgatgcagcg gtactctaag
2760ttcaacaagg aggagcaaga gaacgccgaa ttccttctgc gcgcgtaccc
ggatttgcag 2820atcgcgtacc tcgatgaaga gcccggtccc agcaagagcg
acgaggttcg gttgttttcg 2880acactcatcg atggacactc cgaggtggat
gagaagaccg gccgccgcaa gcccaagttc 2940cgcattgagc tgcccggtaa
ccccatcctc ggtgacggga agtcggataa ccagaaccac 3000gccattgtct
tctaccgcgg cgagtacatc caggtcatcg acgctaacca ggacaattac
3060ctggaagagt gtctcaagat ccgtaacgtc ctgggcgagt ttgaggaata
ctccgtgtcg 3120agccagagcc cgtacgcaca gtggggccac aaggagttca
acaagtgccc cgtcgctatc 3180ctgggttctc gcgagtacat cttctcggag
aacatcggta tcctcggtga catcgccgcc 3240ggcaaggaac agacgttcgg
taccattacg gcgcgtgcgc ttgcgtggat cggcggcaag 3300ctgcattacg
gtcacccgga tttcctcaat gcgacgttca tgacgacgcg tggtggcgtg
3360tcaaaagcgc agaagggctt gcatctcaac gaggatatct tcgctggtat
gaccgccgtg 3420tcccgcggag ggcgcatcaa gcacatggag tactaccagt
gcggcaaagg tcgtgatctc 3480ggtttcggca cgatcttgaa cttccagacg
aagatcggta ctggtatggg cgagcagctc 3540ctctcgcgcg agtactacta
cctgggcacg caattgccta tcgaccggtt cttgacgttc 3600tactacgcgc
acgctggttt ccacgtcaac aacatcctgg tcatctactc catccaggtc
3660ttcatggtca ccttgctgta cctgggcaca ttgaacaagc agctgttcat
ctgcaaggtc 3720aactccaatg gccaggttct tagtggacaa gctgggtgct
acaacctcat cccggtcttc 3780gagtggattc gccggagtat catctccatc
ttcttggtgt tcttcatcgc cttcttgcct 3840ctattcttgc aagagctgtg
cgagcgcgga acgggaaagg cgttgctgcg tctcgggaag 3900cacttcttgt
cactgtcgcc cattttcgaa gtgttctcca cccagattta ctcgcaggcg
3960ctcttgaaca acatgagctt cggtggtgcg cgctacatcg ccacaggtcg
tggtttcgcg 4020actagtcgca tacccttcaa catcctctac tcgcgtttcg
cgccgccaag catctacatg 4080ggcatgcgta acctgctgct cctgctgtac
gcgacgatgg ccatttggat cccgcacctg 4140atctacttct ggttctccgt
cctctccctc tgcatcgcgc cattcatgtt caatccgcat 4200caattctcgt
acgccgactt catcatcgac taccgggagt tcttgcgctg gatgtcgcgc
4260ggtaactcgc gaacgaaggc gagcagctgg tacggatact gccgtctgtc
gcgtaccgcg 4320attactgggt acaagaagaa gaagctggga cacccgtcgg
agaagctgtc gggcgacgta 4380ccgcgtgcgc cgtggaggaa cgttatcttc
tcggagatcc tgtggcccat cggcgcgtgc 4440atcatcttca tcgtcgcgta
catgttcgtc aagtcgttcc ccgacgagca gggcaacgcg 4500ccgccgagcc
cgctggtccg gattctgctc atcgcggttg gccctactgt gtggaacgcg
4560gcggtgctca taacgctgtt cttcctgtcg ctcttcctgg gcccgatgat
ggatggctgg 4620gtcaagttcg gctcggtcat ggcggccctt gcgcatggcc
tggcgcttat aggcatgctc 4680acgttctttg agttcttctg gttccttgag
ctctgggatg cctcgcacgc cgtgctcggc 4740gtcatcgcta tcattgccgt
tcagcgcggg atccagaaga tcctcattgc cgtcttcctg 4800acgcgtgagt
acaagcacga cgagacgaac cgcgcgtggt ggacaggtaa atggtatgga
4860cgcgggctgg gtacctcggc catgtcccag ccggcgcgcg agttcatcgt
gaagatcgtg 4920gagatgtcgt tgtggacgtc ggacttcctg cttgcgcacc
tgttgctcat catcttgacg 4980gtgccgctac tgctgccgtt cttcaactca
attcattcga cgatgctttt ctggttgcgc 5040ccttcgaagc agattaggca
acctctgttc tccaccaagc agaagcggca acggcgatgg 5100attgtcatga
agtataccgt ggtatatctc gtggtggtgg ctttcctcgt cgcgctcatc
5160gctctgcccg ccctcttccg cgagagcatc cacttcaact gcgagatctg
ccagagtata 5220tag 5223141740PRTSchizophyllum commune 14Met Ser Gly
Pro Gly Tyr Gly Arg Asn Pro Phe Asp Asn Pro Pro Pro 1 5 10 15 Asn
Arg Gly Pro Tyr Gly Gln Gln Pro Gly Phe Pro Gly Pro Gly Pro 20 25
30 Arg Pro Tyr Asp Ser Asp Ala Asp Met Ser Gln Thr Tyr Gly Ser Thr
35 40 45 Thr Arg Leu Ala Gly Ser Ala Gly Tyr Ser Asp Arg Asn Gly
Ser Phe 50 55 60 Asp Gly Asp Arg Ser Tyr Ala Pro Ser Ile Asp Ser
Arg Ala Ser Val 65 70 75 80 Pro Ser Ile Ser Pro Phe Ala Asp Pro Gly
Ile Gly Ser Asn Glu Pro 85 90 95 Tyr Pro Ala Trp Ser Val Glu Arg
Gln Ile Pro Met Ser Thr Glu Glu 100 105 110 Ile Glu Asp Ile Phe Leu
Asp Leu Thr Gln Lys Phe Gly Phe Gln Arg 115 120 125 Asp Ser Met Arg
Asn Thr Phe Asp Phe Met Met His Leu Leu Asp Ser 130 135 140 Arg Ala
Ser Arg Met Thr Pro Asn Gln Ala Leu Leu Thr Leu His Ala 145 150 155
160 Asp Tyr Ile Gly Gly Gln His Ala Asn Tyr Arg Lys Trp Tyr Phe Ala
165 170 175 Ala Gln Leu Asn Leu Asp Asp Ala Val Gly Gln Thr Asn Asn
Pro Gly 180 185 190 Ile Gln Arg Leu Lys Thr Ile Lys Gly Ala Thr Lys
Thr Lys Ser Leu 195 200 205 Asp Ser Ala Leu Asn Arg Trp Arg Asn Ala
Met Asn Asn Met Ser Gln 210 215 220 Tyr Asp Arg Leu Arg Gln Ile Ala
Leu Tyr Leu Leu Cys Trp Gly Glu 225 230 235 240 Ala Gly Asn Ile Arg
Leu Ala Pro Glu Cys Leu Cys Phe Ile Phe Lys 245 250 255 Cys Ala Asp
Asp Tyr Tyr Arg Ser Pro Glu Cys Gln Asn Arg Met Asp 260 265 270 Pro
Val Pro Glu Gly Leu Tyr Leu Gln Thr Val Ile Lys Pro Leu Tyr 275 280
285 Arg Phe Leu Arg Asp Gln Ala Tyr Glu Val Val Asp Gly Lys Gln Val
290 295 300 Lys Arg Glu Lys Asp His Asp Gln Ile Ile Gly Tyr Asp Asp
Val Asn 305 310 315 320 Gln Leu Phe Trp Tyr Pro Glu Gly Leu Ala Lys
Ile Val Met Ser Asp 325 330 335 Asn Thr Arg Leu Val Asp Val Pro Pro
Ala Gln Arg Phe Met Lys Phe 340 345 350 Ala Lys Ile Glu Trp Asn Arg
Val Phe Phe Lys Thr Tyr Phe Glu Lys 355 360 365 Arg Ser Thr Ala His
Leu Leu Val Asn Phe Asn Arg Ile Trp Ile Leu 370 375 380 His Val Ser
Met Tyr Phe Phe Tyr Thr Ala Phe Asn Ser Pro Arg Val 385 390 395 400
Tyr Ala Pro His Gly Lys Leu Asp Pro Ser Pro Glu Met Thr Trp Ser 405
410 415 Ala Thr Ala Leu Gly Gly Ala Val Ser Thr Met Ile Met Ile Leu
Ala 420 425 430 Thr Ile Ala Glu Tyr Thr Tyr Ile Pro Thr Thr Trp Asn
Asn Ala Ser 435 440 445 His Leu Thr Thr Arg Leu Ile Phe Leu Leu Val
Ile Leu Ala Leu Thr 450 455 460 Ala Gly Pro Thr Phe Tyr Ile Ala Met
Ile Asp Gly Arg Thr Asp Ile 465 470 475 480 Gly Gln Val Pro Leu Ile
Val Ala Ile Val Gln Phe Phe Ile Ser Val 485 490 495 Val Ala Thr Leu
Ala Phe Ala Thr Ile Pro Ser Gly Arg Met Phe Gly 500 505 510 Asp Arg
Val Ala Gly Lys Ser Arg Lys His Met Ala Ser Gln Thr Phe 515 520 525
Thr Ala Ser Tyr Pro Ser Met Lys Arg Ser Ser Arg Val Ala Ser Ile 530
535 540 Met Leu Trp Leu Leu Val Phe Gly Cys Lys Tyr Val Glu Ser Tyr
Phe 545 550 555 560 Phe Leu Thr Ser Ser Phe Ser Ser Pro Ile Ala Val
Met Ala Arg Thr 565 570 575 Lys Val Gln Gly Cys Asn Asp Arg Ile Phe
Gly Ser Gln Leu Cys Thr 580 585 590 Asn Gln Val Pro Phe Ala Leu Ala
Ile Met Tyr Val Met Asp Leu Val 595 600 605 Leu Phe Phe Leu Asp Thr
Tyr Leu Trp Tyr Ile Ile Trp Leu Val Ile 610 615 620 Phe Ser Met Val
Arg Ala Phe Lys Leu Gly Ile Ser Ile Trp Thr Pro 625 630 635 640 Trp
Ser Glu Ile Phe Thr Arg Met Pro Lys Arg Ile Tyr Ala Lys Leu 645 650
655 Leu Ala Thr Ala Glu Met Glu Val Lys Tyr Lys Pro Lys Val Leu Val
660 665 670 Ser Gln Ile Trp Asn Ala Val Ile Ile Ser Met Tyr Arg Glu
His Leu 675 680 685 Leu Ser Ile Glu His Val Gln Arg Leu Leu Tyr His
Gln Val Asp Gly 690 695 700 Pro Asp Gly Arg Arg Thr Leu Arg Ala Pro
Pro Phe Phe Thr Ser Gln 705 710 715 720 Arg Thr Ala Lys Pro Gly Leu
Phe Phe Pro Pro Gly Gly Glu Ala Glu 725 730 735 Arg Arg Ile Ser Phe
Phe Ala Ser Ser Leu Thr Thr Ala Leu Pro Glu 740 745 750 Pro Leu Pro
Ile Asp Ala Met Pro Thr Phe Thr Val Leu Val Pro His 755 760 765 Tyr
Ser Glu Lys Ile Leu Leu Ser Leu Arg Glu Ile Ile Arg Glu Glu 770 775
780 Asp Gln Asn Thr Arg Val Thr Leu Leu Glu Tyr Leu Lys Gln Leu His
785 790 795 800 Pro Val Glu Trp Asp Asn Phe Val Lys Asp Thr Lys Ile
Leu Ala Glu 805 810 815 Glu Ser Gly Asp Val Gln Asp Glu Lys Arg Ala
Arg Thr Asp Asp Leu 820 825 830 Pro Phe Tyr Cys Ile Gly Phe Lys Thr
Ser Ser Pro Glu Tyr Thr Leu 835 840 845 Arg Thr Arg Ile Trp Ala Ser
Leu Arg Ala Gln Thr Leu Tyr Arg Thr 850 855 860 Val Ser Gly Met Met
Asn Tyr Ser Lys Ala Ile Lys Leu Leu Tyr Arg 865 870 875 880 Val Glu
Asn Pro Asp Val Val His Ala Phe Gly Gly Asn Thr Glu Arg 885 890 895
Leu Glu Arg Glu Leu Glu Arg Met Ser Arg Arg Lys Phe Lys Phe Val 900
905 910 Ile Ser Met Gln Arg Tyr Ser Lys Phe Asn Lys Glu Glu Gln Glu
Asn 915 920 925 Ala Glu Phe Leu Leu Arg Ala Tyr Pro Asp Leu Gln Ile
Ala Tyr Leu 930 935 940 Asp Glu Glu Pro Gly Pro Ser Lys Ser Asp Glu
Val Arg Leu Phe Ser 945 950 955 960 Thr Leu Ile Asp Gly His Ser Glu
Val Asp Glu Lys Thr Gly Arg Arg 965 970 975 Lys Pro Lys Phe Arg Ile
Glu Leu Pro Gly Asn Pro Ile Leu Gly Asp 980 985 990 Gly Lys Ser Asp
Asn Gln Asn His Ala Ile Val Phe Tyr Arg Gly Glu 995 1000 1005 Tyr
Ile Gln Val Ile Asp Ala Asn Gln Asp Asn Tyr Leu Glu Glu 1010 1015
1020 Cys Leu Lys Ile Arg Asn Val Leu Gly Glu Phe Glu Glu Tyr Ser
1025 1030 1035 Val Ser Ser Gln Ser Pro Tyr Ala Gln Trp Gly His Lys
Glu Phe 1040 1045 1050 Asn Lys Cys Pro Val Ala Ile Leu Gly Ser Arg
Glu Tyr Ile Phe 1055 1060 1065 Ser Glu Asn Ile Gly Ile Leu Gly Asp
Ile Ala Ala Gly Lys Glu 1070 1075 1080 Gln Thr Phe Gly Thr Ile Thr
Ala Arg Ala Leu Ala Trp Ile Gly 1085 1090 1095 Gly Lys Leu His Tyr
Gly His Pro Asp Phe Leu Asn Ala Thr Phe 1100 1105 1110 Met Thr Thr
Arg Gly Gly Val Ser Lys Ala Gln Lys Gly Leu His 1115 1120 1125 Leu
Asn Glu Asp Ile Phe Ala Gly Met Thr Ala Val Ser Arg Gly 1130 1135
1140 Gly Arg Ile Lys His Met Glu Tyr Tyr Gln Cys Gly Lys Gly Arg
1145 1150 1155 Asp Leu Gly Phe Gly Thr Ile Leu Asn Phe Gln Thr Lys
Ile Gly 1160 1165 1170 Thr Gly Met Gly Glu Gln Leu Leu Ser Arg Glu
Tyr Tyr Tyr Leu 1175 1180 1185 Gly Thr Gln Leu Pro Ile Asp Arg Phe
Leu Thr Phe Tyr Tyr Ala 1190 1195 1200 His Ala Gly Phe His Val Asn
Asn Ile Leu Val Ile Tyr Ser Ile 1205 1210 1215 Gln Val Phe Met Val
Thr Leu Leu Tyr Leu Gly Thr Leu Asn Lys 1220 1225 1230 Gln Leu Phe
Ile Cys Lys Val Asn Ser Asn Gly Gln Val Leu Ser 1235 1240 1245 Gly
Gln Ala Gly Cys Tyr Asn Leu Ile Pro Val Phe Glu Trp Ile 1250 1255
1260 Arg Arg Ser Ile Ile Ser Ile Phe Leu Val Phe Phe Ile Ala Phe
1265 1270 1275 Leu Pro Leu Phe Leu Gln Glu Leu Cys Glu Arg Gly Thr
Gly Lys 1280 1285 1290 Ala Leu Leu Arg Leu Gly Lys His Phe Leu Ser
Leu Ser Pro Ile 1295 1300 1305 Phe Glu Val Phe Ser Thr Gln Ile Tyr
Ser Gln Ala Leu Leu Asn 1310 1315 1320 Asn Met Ser Phe Gly Gly Ala
Arg Tyr Ile Ala Thr Gly Arg Gly 1325 1330 1335 Phe Ala Thr Ser Arg
Ile Pro Phe Asn Ile Leu Tyr Ser Arg Phe 1340 1345 1350 Ala Pro Pro
Ser Ile Tyr Met Gly Met Arg Asn Leu Leu Leu Leu 1355 1360 1365 Leu
Tyr Ala Thr Met Ala Ile Trp Ile Pro His Leu Ile Tyr Phe 1370 1375
1380 Trp Phe Ser Val Leu Ser Leu Cys Ile Ala Pro Phe Met Phe Asn
1385 1390 1395 Pro His Gln Phe Ser Tyr Ala Asp Phe Ile Ile Asp Tyr
Arg Glu 1400 1405 1410 Phe Leu Arg Trp Met Ser Arg Gly Asn Ser Arg
Thr Lys Ala Ser 1415 1420 1425 Ser Trp Tyr Gly Tyr Cys Arg Leu Ser
Arg Thr Ala Ile Thr Gly 1430 1435 1440 Tyr Lys Lys Lys Lys Leu Gly
His Pro Ser Glu Lys Leu Ser Gly 1445 1450 1455 Asp Val Pro Arg Ala
Pro Trp Arg Asn Val Ile Phe Ser Glu Ile 1460 1465 1470 Leu Trp Pro
Ile Gly Ala Cys Ile Ile Phe Ile Val Ala Tyr Met 1475 1480 1485 Phe
Val Lys Ser Phe Pro Asp Glu Gln Gly Asn Ala Pro Pro Ser 1490 1495
1500 Pro Leu Val Arg Ile Leu Leu Ile Ala Val Gly Pro Thr Val Trp
1505 1510 1515 Asn Ala Ala Val Leu Ile Thr Leu Phe Phe Leu Ser Leu
Phe Leu 1520 1525 1530 Gly Pro Met Met Asp Gly Trp Val Lys Phe Gly
Ser Val Met Ala 1535 1540 1545 Ala Leu Ala His Gly Leu Ala Leu Ile
Gly Met Leu Thr Phe Phe 1550 1555 1560 Glu Phe Phe Trp Phe Leu Glu
Leu Trp Asp Ala Ser His Ala Val 1565 1570 1575 Leu Gly Val Ile Ala
Ile Ile Ala Val Gln Arg Gly Ile Gln Lys 1580 1585 1590 Ile Leu Ile
Ala Val Phe Leu Thr Arg Glu Tyr Lys His Asp Glu 1595 1600 1605 Thr
Asn Arg Ala Trp Trp Thr Gly Lys Trp Tyr Gly Arg Gly Leu 1610 1615
1620 Gly Thr Ser Ala Met Ser Gln Pro Ala Arg Glu Phe Ile Val Lys
1625 1630 1635 Ile Val Glu Met Ser Leu Trp Thr Ser Asp Phe Leu Leu
Ala His 1640 1645 1650 Leu Leu Leu Ile Ile Leu Thr Val Pro Leu Leu
Leu Pro Phe Phe 1655 1660 1665 Asn Ser Ile His Ser Thr Met Leu Phe
Trp Leu Arg Pro Ser Lys 1670 1675 1680 Gln Ile Arg Gln Pro Leu Phe
Ser Thr Lys Gln Lys Arg Gln Arg 1685 1690 1695 Arg Trp Ile Val Met
Lys Tyr Thr Val Val Tyr Leu Val Val Val 1700 1705 1710 Ala Phe Leu
Val Ala Leu Ile Ala Leu Pro Ala Leu Phe Arg Glu 1715 1720 1725 Ser
Ile His Phe Asn Cys Glu Ile Cys Gln Ser Ile 1730 1735 1740 15
5352DNASchizophyllum commune 15atgccgaggc cgggcggcac cagcgcagaa
ggcggctacg catcatcgcc gtcgatggag 60acgaccccca gcgatccctt cggaaccgcg
aacggcgcgc cccgccgcta ctacgacaat 120gattctgagg agtacggacc
tggccgtaga gacacctacg cgtccgacag cagtaatcag 180ggcctcacgg
acccgggcta ctacgaccag aatggcgcct atgatcccta tccgaccggg
240gacaccgatt ccgacggcga cgtctacggc cagcgatatg gaccctcagc
agagtcgctt 300ggcacccaca agttcggcca ttccgattca tccacgccga
cttttgtcga ctacagcgca 360tcctccggcg ggagggattc gtaccctgca
tggactgccg aacgcaacat cccgctgtcc 420aaggaggaga tcgaggacat
cttcctcgat ttgacgcaga agtttggctt tcagcgggat 480tccatgcgga
atatgttcga cttcaccatg cagctgcttg acagccgagc gtctcgtatg
540acccccaacc aggcgctcct caccctccac gccgactaca ttggtggcca
gcatgcgaac 600taccggaagt ggtacttcgc ggcgcagctc gaccttgacg
acgccgtggg acaaactcag 660aatccgggtc tcaaccgcct caagtccact
cgcggatcgg gcaagcgacc acgccatgaa 720aagtcgctga acacggcatt
ggagcgctgg cggcaagcca tgaacaacat gtcgcagtat 780gaccgcttac
gccagatcgc
gctctacctg ctctgctggg gcgaagcggc gcaagtgcga 840ttcatgcccg
agtgcttgtg cttcatcttc aagtgcgccg acgactacta tcgttcgccg
900gagtgccaga acaggatgga gccggtaccg gagggtctct acctgaggac
ggtcgtaaag 960ccgctctaca gatttgtccg ggatcaaggc tatgaggtgg
tggagggaaa attcgtacgg 1020cgggaacggg atcacgacca aatcattggt
tacgatgacg tgaatcagct gttctggtac 1080ccggagggaa ttgcccgtat
cgtcctgtcg gacaagagtc gtctagtcga cctcccccca 1140gcacagcgct
tcatgaagtt cgaccgtatc gagtggaatc gcgtcttctt caagacgttt
1200tacgagactc gatccttcac gcatcttttg gtcgacttca accgtatctg
ggtcgtgcac 1260atcgctctct acttcttcta cactgcatac aactccccca
cgatctacgc catcaacggc 1320aacacaccga cgtctctggc ttggagcgcg
actgcgctcg gcggtgcggt agcgacaggt 1380atcatgatcc tcgccacgat
cgccgagttc tcgcacatcc ccacgacatg gaacaacacc 1440tcgcatctga
ctcgccgcct cgccttcctc ctcgtcacgc tcggcctcac atgtggtccg
1500acgttctacg tcgcgattgc agagagcaac gggagcggcg gctctttggc
cttgattctc 1560ggtatcgtcc agttcttcat ctccgtcgtg gcaactgcgc
tcttcactat catgccttct 1620ggtcgtatgt tcggcgaccg tgtcgcaggc
aagagtcgca agtatctcgc cagccagacg 1680ttcacggcca gctacccgtc
gttgcccaag caccagcggt tcgcctcact cctgatgtgg 1740ttcctcatct
tcgggtgcaa gttgacggag agttacttct ttctgacgct gtccttccgc
1800gaccctatcc gcgtcatggt cggcatgaag atccagaact gcgaggacaa
gattttcggc 1860agcggccttt gcaggaatca cgcagcattc accctcacga
tcatgtacat catggacctc 1920gtcttgttct tcctcgacac cttcctttgg
tatgtcatct ggaactcggt tttcagtatc 1980gcacgctctt tcgtactcgg
cctttcgatc tggacaccgt ggagagacat cttccagcgt 2040ctgccgaagc
ggatctacgc gaagcttctg gcgactggcg acatggaggt caagtacaag
2100cccaaggtct tggtctcgca aatctggaac gccatcatca tctccatgta
ccgcgagcac 2160ttgctctcta ttgagcacgt ccagaagctc ctgtaccacc
aagtggacac tggcgaagcc 2220ggcaagcgga gtcttcgcgc gcctccgttc
ttcgtcgcgc agggcagcag cggtggctcg 2280ggcgagttct tcccgcctgg
cagcgaggcc gagcgtcgta tctctttctt cgcgcagtcg 2340ctttctacgg
agattcctca gcccatcccg gtcgacgcca tgccgacgtt cacggtgctt
2400acgcctcact acagcgagaa gatccttctc tctctccgtg aaattatccg
cgaggaggac 2460cagaacactc gcgttacgtt gctcgagtac ctgaagcagc
tgcatccggt cgagtgggag 2520aatttcgtca aggacactaa aattttggcc
gaggagtccg ctatgtttaa cggtccgagt 2580cctttcggca acgacgagaa
gggtcagtcc aagatggacg atctaccgtt ctactgcatc 2640ggtttcaaga
gcgccgcgcc cgagtacacc ctccgcaccc gtatctgggc gtccctgcgc
2700gcgcagacgc tgtaccgcac ggtctccggc atgatgaact atgcgaaggc
gatcaagctg 2760ctctaccgcg ttgagaaccc ggaggtcgta caacagttcg
gcggcaacac ggacaagctc 2820gagcgcgagt tggagcggat ggcgcgacgg
aagttcaagt tcctcgtgtc catgcagcgc 2880tactcgaagt tcaacaagga
ggagcacgag aacgccgagt tcttgctccg cgcgtacccg 2940gacttgcaga
tcgcgtacct cgaggaagag ccccctcgca aggagggcgg cgatccacgc
3000atcttctctg ccctcgtcga cggccacagc gacatcatcc cggagaccgg
caagcggcgc 3060cccaagttcc gtatcgagct gcccggtaac cccattctcg
gtgacggtaa atccgacaat 3120cagaaccacg ctatcgtctt ctaccgcggc
gagtacctcc agcttatcga cgccaaccag 3180gacaactacc tcgaggagtg
cttgaagatc cgtaacgtgc tcgccgagtt tgaggagtac 3240gacgtctcca
gccagagccc gtacgcgcag tggagtgtca aggagttcaa gcgctctccg
3300gtcgccatcg tcggtgcacg cgagtacatc ttctcagagc acatcggtat
cctcggtgat 3360ctggcggctg gcaaggaaca gacgttcggt acgctcacgg
cacgcaacaa cgccttcctt 3420ggcggcaagc tgcactacgg tcaccccgat
ttcctcaacg ccctctacat gaacacgcgc 3480ggtggtgtct ccaaggcgca
gaagggtctc catctcaacg aggatatcta cgccggtatg 3540aacgcggtcg
gtcgcggtgg acgcattaag cacagcgagt actatcagtg cggcaagggt
3600cgtgacctcg gtttcggcac catcttgaac ttccagacca agatcggtac
gggtatgggc 3660gagcagatcc tctcgcgcga gtactactat ctcggaacac
aactgcccat cgatcgcttc 3720ctcacgttct actacgcgca cccgggtttc
cagatcaaca acatgctggt catcctctcc 3780gtgcaggtct tcatcgttac
catggtcttc ctcggtacct tgaagtcttc ggtcacgatc 3840tgcaagtaca
cgtccagcgg tcagtacatc ggtggtcaat ccggttgcta caacctcgtc
3900ccggtcttcc agtggatcga gcgctgcatc atcagcatct tcttggtgtt
catgatcgct 3960ttcatgccgc tcttcctgca agaactcgtc gagcgcggta
cctggagtgc catctggcgt 4020ctgctcaagc agtttatgtc gctgtcgcct
gtcttcgagg tgttctccac ccagattcag 4080acgcactccg tgttgagcaa
cttgacgttc ggtggtgcgc gttacatcgc taccggtcgt 4140gggttcgcca
ccagtcgtat cagcttcagc atcttgttct cgcgtttcgc aggcccgagt
4200atctacctcg gcatgcgcac gctcattatg ctgctctacg tgacgttgac
gatctggacg 4260ccatgggtca tttacttctg ggtttccatt ctctcgctct
gcatcgcgcc gttcttgttc 4320aacccgcatc aattcgtatt ctcggacttc
ctcatcgact acagggaata cctgcggtgg 4380atgtcgcgtg gcaactcgcg
ctcgcacaac aactcctgga ttgggtactg ccggttgtcc 4440cgcacgatga
tcactgggta caagaagaag aagctgggcc acccgtcgga gaagctttcc
4500ggcgacgttc ctcgtgcagg ctggcgcgcc gtcttgttct cggagatcat
cttcccggcg 4560tgcatggcca tcctcttcat catcgcgtac atgttcgtca
agtcgttccc tctcgacggc 4620aagcagcctc cctccggcct cgttcgcatc
gccgtcgtgt ctatcggccc catcgtgtgg 4680aacgccgcca tcctgttgac
gctcttcctt gtgtcgttgt tcctcggccc catgctcgac 4740ccggtcttcc
ccctcttcgg ttccgttatg gccttcatcg cgcatttcct tggcacaatc
4800ggaatgattg ggttcttcga gttcctgtgg ttcctcgagt cctgggaggc
gtcgcatgcc 4860gtgctgggtc tcatcgccgt catctccatc cagcgcgcca
ttcacaagat ccttatcgcc 4920gttttcctca gtcgcgagtt caagcacgac
gagacgaaca gggcctggtg gactggtcgc 4980tggtatggcc gtggcctcgg
cacgcacgcc atgtcgcagc cggcgcgtga gttcgtcgtc 5040aagatcatcg
agttgtcgct ttggagctcg gatctcatac tcggccacat cctgctgttc
5100atgcttactc cggccgtcct catcccgtac ttcgaccgtt tgcacgccat
gatgctcttc 5160tggctgcgtc cctcgaagca aatccgcgcg cctctgtact
cgatcaagca gaagaggcaa 5220agacgctgga ttatcatgaa gtacggtact
gtatacgtta ccgtcatcgc gatcttcgtc 5280gcgctcatcg cgcttcccct
cgtattccga cacactctaa aggtcgagtg ctccctttgc 5340gacagcttgt aa
5352161783PRTSchizophyllum commune 16Met Pro Arg Pro Gly Gly Thr
Ser Ala Glu Gly Gly Tyr Ala Ser Ser 1 5 10 15 Pro Ser Met Glu Thr
Thr Pro Ser Asp Pro Phe Gly Thr Ala Asn Gly 20 25 30 Ala Pro Arg
Arg Tyr Tyr Asp Asn Asp Ser Glu Glu Tyr Gly Pro Gly 35 40 45 Arg
Arg Asp Thr Tyr Ala Ser Asp Ser Ser Asn Gln Gly Leu Thr Asp 50 55
60 Pro Gly Tyr Tyr Asp Gln Asn Gly Ala Tyr Asp Pro Tyr Pro Thr Gly
65 70 75 80 Asp Thr Asp Ser Asp Gly Asp Val Tyr Gly Gln Arg Tyr Gly
Pro Ser 85 90 95 Ala Glu Ser Leu Gly Thr His Lys Phe Gly His Ser
Asp Ser Ser Thr 100 105 110 Pro Thr Phe Val Asp Tyr Ser Ala Ser Ser
Gly Gly Arg Asp Ser Tyr 115 120 125 Pro Ala Trp Thr Ala Glu Arg Asn
Ile Pro Leu Ser Lys Glu Glu Ile 130 135 140 Glu Asp Ile Phe Leu Asp
Leu Thr Gln Lys Phe Gly Phe Gln Arg Asp 145 150 155 160 Ser Met Arg
Asn Met Phe Asp Phe Thr Met Gln Leu Leu Asp Ser Arg 165 170 175 Ala
Ser Arg Met Thr Pro Asn Gln Ala Leu Leu Thr Leu His Ala Asp 180 185
190 Tyr Ile Gly Gly Gln His Ala Asn Tyr Arg Lys Trp Tyr Phe Ala Ala
195 200 205 Gln Leu Asp Leu Asp Asp Ala Val Gly Gln Thr Gln Asn Pro
Gly Leu 210 215 220 Asn Arg Leu Lys Ser Thr Arg Gly Ser Gly Lys Arg
Pro Arg His Glu 225 230 235 240 Lys Ser Leu Asn Thr Ala Leu Glu Arg
Trp Arg Gln Ala Met Asn Asn 245 250 255 Met Ser Gln Tyr Asp Arg Leu
Arg Gln Ile Ala Leu Tyr Leu Leu Cys 260 265 270 Trp Gly Glu Ala Ala
Gln Val Arg Phe Met Pro Glu Cys Leu Cys Phe 275 280 285 Ile Phe Lys
Cys Ala Asp Asp Tyr Tyr Arg Ser Pro Glu Cys Gln Asn 290 295 300 Arg
Met Glu Pro Val Pro Glu Gly Leu Tyr Leu Arg Thr Val Val Lys 305 310
315 320 Pro Leu Tyr Arg Phe Val Arg Asp Gln Gly Tyr Glu Val Val Glu
Gly 325 330 335 Lys Phe Val Arg Arg Glu Arg Asp His Asp Gln Ile Ile
Gly Tyr Asp 340 345 350 Asp Val Asn Gln Leu Phe Trp Tyr Pro Glu Gly
Ile Ala Arg Ile Val 355 360 365 Leu Ser Asp Lys Ser Arg Leu Val Asp
Leu Pro Pro Ala Gln Arg Phe 370 375 380 Met Lys Phe Asp Arg Ile Glu
Trp Asn Arg Val Phe Phe Lys Thr Phe 385 390 395 400 Tyr Glu Thr Arg
Ser Phe Thr His Leu Leu Val Asp Phe Asn Arg Ile 405 410 415 Trp Val
Val His Ile Ala Leu Tyr Phe Phe Tyr Thr Ala Tyr Asn Ser 420 425 430
Pro Thr Ile Tyr Ala Ile Asn Gly Asn Thr Pro Thr Ser Leu Ala Trp 435
440 445 Ser Ala Thr Ala Leu Gly Gly Ala Val Ala Thr Gly Ile Met Ile
Leu 450 455 460 Ala Thr Ile Ala Glu Phe Ser His Ile Pro Thr Thr Trp
Asn Asn Thr 465 470 475 480 Ser His Leu Thr Arg Arg Leu Ala Phe Leu
Leu Val Thr Leu Gly Leu 485 490 495 Thr Cys Gly Pro Thr Phe Tyr Val
Ala Ile Ala Glu Ser Asn Gly Ser 500 505 510 Gly Gly Ser Leu Ala Leu
Ile Leu Gly Ile Val Gln Phe Phe Ile Ser 515 520 525 Val Val Ala Thr
Ala Leu Phe Thr Ile Met Pro Ser Gly Arg Met Phe 530 535 540 Gly Asp
Arg Val Ala Gly Lys Ser Arg Lys Tyr Leu Ala Ser Gln Thr 545 550 555
560 Phe Thr Ala Ser Tyr Pro Ser Leu Pro Lys His Gln Arg Phe Ala Ser
565 570 575 Leu Leu Met Trp Phe Leu Ile Phe Gly Cys Lys Leu Thr Glu
Ser Tyr 580 585 590 Phe Phe Leu Thr Leu Ser Phe Arg Asp Pro Ile Arg
Val Met Val Gly 595 600 605 Met Lys Ile Gln Asn Cys Glu Asp Lys Ile
Phe Gly Ser Gly Leu Cys 610 615 620 Arg Asn His Ala Ala Phe Thr Leu
Thr Ile Met Tyr Ile Met Asp Leu 625 630 635 640 Val Leu Phe Phe Leu
Asp Thr Phe Leu Trp Tyr Val Ile Trp Asn Ser 645 650 655 Val Phe Ser
Ile Ala Arg Ser Phe Val Leu Gly Leu Ser Ile Trp Thr 660 665 670 Pro
Trp Arg Asp Ile Phe Gln Arg Leu Pro Lys Arg Ile Tyr Ala Lys 675 680
685 Leu Leu Ala Thr Gly Asp Met Glu Val Lys Tyr Lys Pro Lys Val Leu
690 695 700 Val Ser Gln Ile Trp Asn Ala Ile Ile Ile Ser Met Tyr Arg
Glu His 705 710 715 720 Leu Leu Ser Ile Glu His Val Gln Lys Leu Leu
Tyr His Gln Val Asp 725 730 735 Thr Gly Glu Ala Gly Lys Arg Ser Leu
Arg Ala Pro Pro Phe Phe Val 740 745 750 Ala Gln Gly Ser Ser Gly Gly
Ser Gly Glu Phe Phe Pro Pro Gly Ser 755 760 765 Glu Ala Glu Arg Arg
Ile Ser Phe Phe Ala Gln Ser Leu Ser Thr Glu 770 775 780 Ile Pro Gln
Pro Ile Pro Val Asp Ala Met Pro Thr Phe Thr Val Leu 785 790 795 800
Thr Pro His Tyr Ser Glu Lys Ile Leu Leu Ser Leu Arg Glu Ile Ile 805
810 815 Arg Glu Glu Asp Gln Asn Thr Arg Val Thr Leu Leu Glu Tyr Leu
Lys 820 825 830 Gln Leu His Pro Val Glu Trp Glu Asn Phe Val Lys Asp
Thr Lys Ile 835 840 845 Leu Ala Glu Glu Ser Ala Met Phe Asn Gly Pro
Ser Pro Phe Gly Asn 850 855 860 Asp Glu Lys Gly Gln Ser Lys Met Asp
Asp Leu Pro Phe Tyr Cys Ile 865 870 875 880 Gly Phe Lys Ser Ala Ala
Pro Glu Tyr Thr Leu Arg Thr Arg Ile Trp 885 890 895 Ala Ser Leu Arg
Ala Gln Thr Leu Tyr Arg Thr Val Ser Gly Met Met 900 905 910 Asn Tyr
Ala Lys Ala Ile Lys Leu Leu Tyr Arg Val Glu Asn Pro Glu 915 920 925
Val Val Gln Gln Phe Gly Gly Asn Thr Asp Lys Leu Glu Arg Glu Leu 930
935 940 Glu Arg Met Ala Arg Arg Lys Phe Lys Phe Leu Val Ser Met Gln
Arg 945 950 955 960 Tyr Ser Lys Phe Asn Lys Glu Glu His Glu Asn Ala
Glu Phe Leu Leu 965 970 975 Arg Ala Tyr Pro Asp Leu Gln Ile Ala Tyr
Leu Glu Glu Glu Pro Pro 980 985 990 Arg Lys Glu Gly Gly Asp Pro Arg
Ile Phe Ser Ala Leu Val Asp Gly 995 1000 1005 His Ser Asp Ile Ile
Pro Glu Thr Gly Lys Arg Arg Pro Lys Phe 1010 1015 1020 Arg Ile Glu
Leu Pro Gly Asn Pro Ile Leu Gly Asp Gly Lys Ser 1025 1030 1035 Asp
Asn Gln Asn His Ala Ile Val Phe Tyr Arg Gly Glu Tyr Leu 1040 1045
1050 Gln Leu Ile Asp Ala Asn Gln Asp Asn Tyr Leu Glu Glu Cys Leu
1055 1060 1065 Lys Ile Arg Asn Val Leu Ala Glu Phe Glu Glu Tyr Asp
Val Ser 1070 1075 1080 Ser Gln Ser Pro Tyr Ala Gln Trp Ser Val Lys
Glu Phe Lys Arg 1085 1090 1095 Ser Pro Val Ala Ile Val Gly Ala Arg
Glu Tyr Ile Phe Ser Glu 1100 1105 1110 His Ile Gly Ile Leu Gly Asp
Leu Ala Ala Gly Lys Glu Gln Thr 1115 1120 1125 Phe Gly Thr Leu Thr
Ala Arg Asn Asn Ala Phe Leu Gly Gly Lys 1130 1135 1140 Leu His Tyr
Gly His Pro Asp Phe Leu Asn Ala Leu Tyr Met Asn 1145 1150 1155 Thr
Arg Gly Gly Val Ser Lys Ala Gln Lys Gly Leu His Leu Asn 1160 1165
1170 Glu Asp Ile Tyr Ala Gly Met Asn Ala Val Gly Arg Gly Gly Arg
1175 1180 1185 Ile Lys His Ser Glu Tyr Tyr Gln Cys Gly Lys Gly Arg
Asp Leu 1190 1195 1200 Gly Phe Gly Thr Ile Leu Asn Phe Gln Thr Lys
Ile Gly Thr Gly 1205 1210 1215 Met Gly Glu Gln Ile Leu Ser Arg Glu
Tyr Tyr Tyr Leu Gly Thr 1220 1225 1230 Gln Leu Pro Ile Asp Arg Phe
Leu Thr Phe Tyr Tyr Ala His Pro 1235 1240 1245 Gly Phe Gln Ile Asn
Asn Met Leu Val Ile Leu Ser Val Gln Val 1250 1255 1260 Phe Ile Val
Thr Met Val Phe Leu Gly Thr Leu Lys Ser Ser Val 1265 1270 1275 Thr
Ile Cys Lys Tyr Thr Ser Ser Gly Gln Tyr Ile Gly Gly Gln 1280 1285
1290 Ser Gly Cys Tyr Asn Leu Val Pro Val Phe Gln Trp Ile Glu Arg
1295 1300 1305 Cys Ile Ile Ser Ile Phe Leu Val Phe Met Ile Ala Phe
Met Pro 1310 1315 1320 Leu Phe Leu Gln Glu Leu Val Glu Arg Gly Thr
Trp Ser Ala Ile 1325 1330 1335 Trp Arg Leu Leu Lys Gln Phe Met Ser
Leu Ser Pro Val Phe Glu 1340 1345 1350 Val Phe Ser Thr Gln Ile Gln
Thr His Ser Val Leu Ser Asn Leu 1355 1360 1365 Thr Phe Gly Gly Ala
Arg Tyr Ile Ala Thr Gly Arg Gly Phe Ala 1370 1375 1380 Thr Ser Arg
Ile Ser Phe Ser Ile Leu Phe Ser Arg Phe Ala Gly 1385 1390 1395 Pro
Ser Ile Tyr Leu Gly Met Arg Thr Leu Ile Met Leu Leu Tyr 1400 1405
1410 Val Thr Leu Thr Ile Trp Thr Pro Trp Val Ile Tyr Phe Trp Val
1415 1420 1425 Ser Ile Leu Ser Leu Cys Ile Ala Pro Phe Leu Phe Asn
Pro His 1430 1435 1440 Gln Phe Val Phe Ser Asp Phe Leu Ile Asp Tyr
Arg Glu Tyr Leu 1445 1450 1455 Arg Trp Met Ser Arg Gly Asn Ser Arg
Ser His Asn Asn Ser Trp 1460 1465 1470 Ile Gly Tyr Cys Arg Leu Ser
Arg Thr Met Ile Thr Gly Tyr Lys 1475 1480 1485 Lys Lys Lys Leu Gly
His Pro Ser Glu Lys Leu Ser Gly Asp Val 1490 1495 1500 Pro Arg Ala
Gly Trp Arg Ala Val Leu Phe Ser Glu Ile Ile Phe 1505 1510 1515 Pro
Ala Cys Met Ala Ile Leu Phe Ile Ile Ala Tyr Met Phe Val 1520 1525
1530 Lys Ser Phe Pro Leu Asp Gly Lys Gln Pro Pro Ser Gly Leu Val
1535 1540 1545 Arg Ile Ala Val Val Ser Ile Gly Pro Ile Val Trp Asn
Ala Ala 1550 1555 1560 Ile Leu Leu Thr Leu Phe Leu Val Ser Leu Phe
Leu
Gly Pro Met 1565 1570 1575 Leu Asp Pro Val Phe Pro Leu Phe Gly Ser
Val Met Ala Phe Ile 1580 1585 1590 Ala His Phe Leu Gly Thr Ile Gly
Met Ile Gly Phe Phe Glu Phe 1595 1600 1605 Leu Trp Phe Leu Glu Ser
Trp Glu Ala Ser His Ala Val Leu Gly 1610 1615 1620 Leu Ile Ala Val
Ile Ser Ile Gln Arg Ala Ile His Lys Ile Leu 1625 1630 1635 Ile Ala
Val Phe Leu Ser Arg Glu Phe Lys His Asp Glu Thr Asn 1640 1645 1650
Arg Ala Trp Trp Thr Gly Arg Trp Tyr Gly Arg Gly Leu Gly Thr 1655
1660 1665 His Ala Met Ser Gln Pro Ala Arg Glu Phe Val Val Lys Ile
Ile 1670 1675 1680 Glu Leu Ser Leu Trp Ser Ser Asp Leu Ile Leu Gly
His Ile Leu 1685 1690 1695 Leu Phe Met Leu Thr Pro Ala Val Leu Ile
Pro Tyr Phe Asp Arg 1700 1705 1710 Leu His Ala Met Met Leu Phe Trp
Leu Arg Pro Ser Lys Gln Ile 1715 1720 1725 Arg Ala Pro Leu Tyr Ser
Ile Lys Gln Lys Arg Gln Arg Arg Trp 1730 1735 1740 Ile Ile Met Lys
Tyr Gly Thr Val Tyr Val Thr Val Ile Ala Ile 1745 1750 1755 Phe Val
Ala Leu Ile Ala Leu Pro Leu Val Phe Arg His Thr Leu 1760 1765 1770
Lys Val Glu Cys Ser Leu Cys Asp Ser Leu 1775 1780
17620DNASchizophyllum commune 17atcgccattg taagccgcag acgggcacgc
ttccaacccc catcgatggg cgctcgatgt 60ccatctcatc ggcgactcat cattgtatct
cgcgcagtcc catccctcgc cgctcgcctg 120tagtttatgc tatttatctt
tgcaccagtc gttgtattac tccctcgtcg tgtagaaagt 180accagataaa
atgcatgtaa tcctaatgaa atttgcacga cacgaagatc cggcagggtt
240gtgggcaagg ggcagcggga acgaatggat ggcggggtac agcgagtacc
cggcagtgcc 300acagtcagtg tcacacacgt gactgattgt ccattagcgt
gaccgataac atcgatcaaa 360aattttattt cagaggacga taaataaggg
ccgacggtgc gcgtccgtct ttctctcaac 420cctcatcttc ctctcgtctc
tcactcttcc cccctccacc actaccaagt aagttcaaac 480ttcctctcat
cgcctttgca cacatcgcct acgccccatc tctctccatc tgcctcgcga
540acggcgcccc catcgtcgct ttcccgcgcg agatcttgtg cgatctagtt
tactgacaat 600ctcacctaga aaacatcaaa 62018440DNASchizophyllum
commune 18atccaagtcc ggtggcaagg tcaccaagtc cgccgagaag gccgccaaga
agaagtaaat 60gtagatgtac atatgtattt tctcattccg tttccttcct cttgttgttg
tttcactggt 120cctctcgtgc tcgctcgcat cgcatacagc cattgttgtc
accactataa cttcacgcat 180tctgtatttc atgccaggcg acggggtgtt
cctgccaggc ctgtcgcttg ttgtaacgct 240aatgaaaagt cacgagtagt
ggacgaacga cgatgtattt ctatgtgctg tagcgattat 300ccatttcgag
ttcgccatcg agctctcttc aaacctaggt gcgacgttgt gaatgcagta
360gcaagtgcag agtattgcag actcgtccat tgatgataac ttcaagctac
gtcagagcca 420gatgctactg aacccgggcc 4401936DNAArtificial
sequenceUra_forw (NotI) primer 19ataagaatgc ggccgctcca gctcgacctt
gcgccg 362030DNAArtificial sequenceUra_rev (XbaI) primer
20ctagtctaga ggatccgacg tggaggagcc 302130DNAArtificial
sequenceTefP_forw (XbaI) primer 21ctagtctaga atcgccattg taagccgcag
302230DNAArtificial sequenceTefP_rev (SpeI) primer 22ctagactagt
tttgatgttt tctaggtgag 302330DNAArtificial sequenceTefT_forw (SalI)
primer 23acgcgtcgac caagtccggt ggcaaggtca 302431DNAArtificial
sequenceTefT_rev (SalI) primer 24ccgacgtcga cgggttcagt agcatctggc t
312532DNAArtificial sequenceTefT_forw (EcoRV) primer 25catggtgata
tccaagtccg gtggcaaggt ca 322632DNAArtificial sequenceTefT_rev
(ApaI) primer 26ccgtatgggc ccgggttcag tagcatctgg ct
322730DNAArtificial sequenceGS1_forw (SpeI) primer 27ctagactagt
cccgtccctc aaggccgttc 302836DNAArtificial sequenceGS1_rev (SalI)
primer 28aatggccgac gtcgacatgg tatatgcaat gctatg
362930DNAArtificial sequenceFusion TefP_GS1_forw (XbaI) primer
29ctagtctaga atcgccattg taagccgcag 303036DNAArtificial
sequenceFusion TefP_GS1_rev (SalI) primer 30aatggccgac gtcgacatgg
tatatgcaat gctatg 363130DNAArtificial sequenceGS2_forw (SpeI)
primer 31ctagactagt ctgtccaaag aagagatcga 303233DNAArtificial
sequenceGS2_rev (EcoRV) primer 32tacatgcgat atcttttatg cagactctcc
ctg 33331614DNASchizophyllum commune 33tccagctcga ccttgcgccg
cttggagtaa cgttcagcgt cttcgtcgtc ctcgtcgcgc 60tcgtgtacga tgatgggctc
agccatggca ggtatacaag ctcagagtca atgggggacg 120aggtctcaag
ccgtgaaagt cgtcgtcgaa caacgtcaag ttcgagacgg accagagttg
180gatttcgtga ttagatctac gctcgatcac agaatgatca aagaacaaag
cttgccaaaa 240ggggatctcc catcaacttc aacttgcccc aaaccatcat
gaccgccgct cataagctca 300catacggtca gcgcgctgca aggttcacca
atcccgcggc gaaagccctg ctggaaacca 360tggagcgcaa gaagagcaat
ctatccgtca gcgtcgacgt cgtaaaatcc gccgatctgc 420tcgctattgt
cgataccgtc gggccctata tctgtctgat aaaggcattg cactgtcgct
480tgcggtcttg ggatgctgct tatactctat gaagacccat gtggatgttg
tcgaagactt 540cgactcgtcg ctcgtcacca agcttcaggc tctggccgag
aagcatgatt tcctcatctt 600tgaggacaga aaattcgccg acataggtct
gtccgtcgaa tctctatcga tgtcaactct 660gatgacttgc acaggcaaca
ccgtcgctct gcagtactct agtggcgtgc acaaaattgc 720cagctggtcg
cacatcacga acgcacaccc tgttccagga ccgtcaatca tcagtggcct
780cgcatcggta ggacaacccc tcggtcgcgg actcctcctg ctcgcagaga
tgagcacgaa 840gggctcactt gcgacaggcg cgtacactga agccgccgtc
cagatggcaa gggagaaccg 900cggcttcgtc atcgggttca tcgcccaacg
gcggatggat ggtattggcg cgcctccagg 960ggtgaatgtc gaggacgagg
attttcttgt cttgacacca ggtgtcggac tcgatgtgaa 1020gggcgatggg
atggggcagc aatacaggac gccgaagcaa gtggtacagg aagatgggtg
1080cgatgtaatc atcgtgggtc gcgggattta tggcaaggac ccatcgaagg
tggaagagat 1140acggaggcag gcagagcgtt accaggctgc aggatgggcg
gcgtacattg agagggtcaa 1200cgccttggta tagctaatct gatcggtgtt
gtcttgttaa gcgtcaggct caatggaacg 1260ctttggacga gcggagagta
acttgaatta gcagtgtata cttcgggcaa atcaatcgtg 1320ataaatacaa
gagcacgctc acgcacgtcc aatctccctc aaaatctcca tctttctcgc
1380ctcattcacc ttcctgaacc cagccggcga catctcgaac agaccatgcc
cacccgacag 1440cgcacgcagc ctattcgagt agtccagcat ccggctgagc
ggcgccaccg cctgcaccgc 1500gcgcttcatc ttcacgcccg ccgcctccct
cgccgcagtg ccgccagagg gcgacaccca 1560ctccgggggc acgtacacgc
cgtccgcagg gtacggctcc tccacgtcgg atcc 161434278PRTSchizophyllum
commune 34Met Thr Ala Ala His Lys Leu Thr Tyr Gly Gln Arg Ala Ala
Arg Phe 1 5 10 15 Thr Asn Pro Ala Ala Lys Ala Leu Leu Glu Thr Met
Glu Arg Lys Lys 20 25 30 Ser Asn Leu Ser Val Ser Val Asp Val Val
Lys Ser Ala Asp Leu Leu 35 40 45 Ala Ile Val Asp Thr Val Gly Pro
Tyr Ile Cys Leu Ile Lys Thr His 50 55 60 Val Asp Val Val Glu Asp
Phe Asp Ser Ser Leu Val Thr Lys Leu Gln 65 70 75 80 Ala Leu Ala Glu
Lys His Asp Phe Leu Ile Phe Glu Asp Arg Lys Phe 85 90 95 Ala Asp
Ile Gly Asn Thr Val Ala Leu Gln Tyr Ser Ser Gly Val His 100 105 110
Lys Ile Ala Ser Trp Ser His Ile Thr Asn Ala His Pro Val Pro Gly 115
120 125 Pro Ser Ile Ile Ser Gly Leu Ala Ser Val Gly Gln Pro Leu Gly
Arg 130 135 140 Gly Leu Leu Leu Leu Ala Glu Met Ser Thr Lys Gly Ser
Leu Ala Thr 145 150 155 160 Gly Ala Tyr Thr Glu Ala Ala Val Gln Met
Ala Arg Glu Asn Arg Gly 165 170 175 Phe Val Ile Gly Phe Ile Ala Gln
Arg Arg Met Asp Gly Ile Gly Ala 180 185 190 Pro Pro Gly Val Asn Val
Glu Asp Glu Asp Phe Leu Val Leu Thr Pro 195 200 205 Gly Val Gly Leu
Asp Val Lys Gly Asp Gly Met Gly Gln Gln Tyr Arg 210 215 220 Thr Pro
Lys Gln Val Val Gln Glu Asp Gly Cys Asp Val Ile Ile Val 225 230 235
240 Gly Arg Gly Ile Tyr Gly Lys Asp Pro Ser Lys Val Glu Glu Ile Arg
245 250 255 Arg Gln Ala Glu Arg Tyr Gln Ala Ala Gly Trp Ala Ala Tyr
Ile Glu 260 265 270 Arg Val Asn Ala Leu Val 275
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