U.S. patent application number 15/053421 was filed with the patent office on 2016-06-16 for peptides derived from campylobacter jejuni and their use in vaccination.
The applicant listed for this patent is Evaxion Biotech ApS. Invention is credited to Andreas Holm Mattsson, Niels Iversen Moller.
Application Number | 20160168208 15/053421 |
Document ID | / |
Family ID | 48081403 |
Filed Date | 2016-06-16 |
United States Patent
Application |
20160168208 |
Kind Code |
A1 |
Moller; Niels Iversen ; et
al. |
June 16, 2016 |
Peptides derived from Campylobacter jejuni and their use in
vaccination
Abstract
Disclosed are polypeptides for Campylobacter jejuni that are
useful as immunogenic agents for vaccine use. Also disclosed are
nucleic acid fragments encoding the polypeptides as well as
compositions, methods and molecular biology tools derived from or
related to the proteins.
Inventors: |
Moller; Niels Iversen;
(Copenhagen K, DK) ; Mattsson; Andreas Holm;
(Copenhagen N, DK) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Evaxion Biotech ApS |
Copenhagen N |
|
DK |
|
|
Family ID: |
48081403 |
Appl. No.: |
15/053421 |
Filed: |
February 25, 2016 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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14351423 |
Apr 11, 2014 |
9273102 |
|
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PCT/EP2012/070282 |
Oct 12, 2012 |
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15053421 |
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61550494 |
Oct 24, 2011 |
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Current U.S.
Class: |
424/190.1 ;
435/320.1; 530/324; 530/350; 536/23.7 |
Current CPC
Class: |
C07K 14/205 20130101;
A61K 39/00 20130101; A61K 2039/55505 20130101; A61K 39/105
20130101; A61K 2039/53 20130101; A61K 39/39 20130101; A61K 39/02
20130101 |
International
Class: |
C07K 14/205 20060101
C07K014/205; A61K 39/39 20060101 A61K039/39; A61K 39/02 20060101
A61K039/02 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 12, 2011 |
DK |
PA201100789 |
Claims
1. A polypeptide comprising a) an amino acid sequence selected from
the group consisting of any one of SEQ ID NOs: 1-30, or b) an amino
acid sequence consisting of at least 20 contiguous amino acid
residues from any one of SEQ ID NOs: 1-30, or c) an amino acid
sequence having a sequence identity of at least 80% with the amino
acid sequence of a), or d) an amino acid sequence having a sequence
identity of at least 80% with the amino acid sequence of b), said
polypeptide being antigenic in a mammal.
2. The polypeptide according to claim 1, wherein the at least 20
contiguous amino acid residues has an N-terminal amino acid residue
corresponding to any one of amino acid residues 1, 2, 3, 4, 5, 6,
7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,
24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40,
41, 42, 43, 44, 45, 46, and 47 in any one of SEQ ID NOs: 1-30.
3. The polypeptide according to claim 1, wherein the at least 20
contiguous amino acid residues has an N-terminal amino acid residue
corresponding to any one of amino acid residues 48, 49, 50, 51, 52,
53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,
70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,
87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102,
103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,
116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,
129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,
142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,
155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167,
168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,
181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193,
194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,
207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219,
220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,
233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,
246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, and 258
in any one of SEQ ID NOs: 13-30.
4. The polypeptide according to claim 1, which is fused or
conjugated to an immunogenic carrier molecule.
5. The polypeptide according to claim 4, wherein the immunogenic
carrier molecule is a polypeptide that induces T-helper lymphocyte
responses in a majority of humans.
6. A pharmaceutical composition comprising a polypeptide and a
pharmaceutically acceptable carrier, vehicle or diluent, and which
further comprises an immunological adjuvant, said polypeptide
comprising: a) an amino acid sequence selected from the group
consisting of any one of SEQ ID NOs: 1-30, or b) an amino acid
sequence consisting of at least 20 contiguous amino acid residues
from any one of SEQ ID NOs: 1-30, or c) an amino acid sequence
having a sequence identity of at least 80% with the amino acid
sequence of a), or d) an amino acid sequence having a sequence
identity of at least 80% with the amino acid sequence of b).
7. The pharmaceutical composition according to claim 6, wherein the
adjuvant is an aluminium based adjuvant.
8. A method for inducing immunity in an animal by administering at
least once an immunogenically effective amount of a polypeptide,
said polypeptide comprising: a) an amino acid sequence selected
from the group consisting of any one of SEQ ID NOs: 1-30, or b) an
amino acid sequence consisting of at least 20 contiguous amino acid
residues from any one of SEQ ID NOs: 1-30, or c) an amino acid
sequence having a sequence identity of at least 80% with the amino
acid sequence of a), or d) an amino acid sequence having a sequence
identity of at least 80% with the amino acid sequence of b), or a
pharmaceutical composition comprising said polypeptide so as to
induce adaptive immunity against C. jejuni in the animal.
9. The method according to claim 8, wherein the animal receives
between 0.5 and 5,000 .mu.g of the polypeptide according to claim 1
per administration.
10. The method according to claim 8, wherein the animal is a human
being.
11. The method according to claim 8, wherein the immunity is
effective in reducing the risk of attracting infection with C.
jejuni or is effective in treating or ameliorating infection with
C. jejuni.
12. An isolated nucleic acid fragment, which comprises i) a
nucleotide sequence encoding a polypeptide, said polypeptide
comprising: a) an amino acid sequence selected from the group
consisting of any one of SEQ ID NOs: 1-30, or b) an amino acid
sequence consisting of at least 20 contiguous amino acid residues
from any one of SEQ ID NOs: 1-30, or c) an amino acid sequence
having a sequence identity of at least 80% with the amino acid
sequence of a), or d) an amino acid sequence having a sequence
identity of at least 80% with the amino acid sequence of b) or ii)
a nucleotide sequence consisting of the amino acid encoding part of
any one of SEQ ID NOs: 30-90, iii) a nucleotide sequence consisting
of at least 15 consecutive nucleotides in the amino acid encoding
part of any one of SEQ ID NOs: 30-90, iv) a nucleotide sequence
having a sequence identity of at least 80% with the nucleotide
sequence in ii), v) a nucleotide sequence having a sequence
identity of at least 80% with the nucleotide sequence in iii), vi)
a nucleotide sequence complementary to the nucleotide sequence in
i)-v), or vii) a nucleotide sequence which hybridizes under
stringent conditions with the nucleotide sequence in i)-vi).
13. The nucleic acid fragment according to claim 12, which is a DNA
fragment or an RNA fragment.
14. The nucleic acid fragment according to claim 12, wherein the
sequence identity with the nucleotide sequence in ii) or iii) is at
least 85%, at least 90%, at least 91%, at least 92%, at least 93%,
at least 94%, at least 95%, at least 96%, at least 97%, at least
98%, and at least 99%.
15. A vector comprising a nucleic acid fragment, said nucleic acid
fragment comprising: i) a nucleotide sequence encoding a
polypeptide, said polypeptide comprising: a) an amino acid sequence
selected from the group consisting of any one of SEQ ID NOs: 1-30,
or b) an amino acid sequence consisting of at least 20 contiguous
amino acid residues from any one of SEQ ID NOs: 1-30, or c) an
amino acid sequence having a sequence identity of at least 80% with
the amino acid sequence of a), or d) an amino acid sequence having
a sequence identity of at least 80% with the amino acid sequence of
b) or ii) a nucleotide sequence consisting of the amino acid
encoding part of any one of SEQ ID NOs: 30-90, iii) a nucleotide
sequence consisting of at least 15 consecutive nucleotides in the
amino acid encoding part of any one of SEQ ID NOs: 30-90, iv) a
nucleotide sequence having a sequence identity of at least 80% with
the nucleotide sequence in ii), v) a nucleotide sequence having a
sequence identity of at least 80% with the nucleotide sequence in
iii), vi) a nucleotide sequence complementary to the nucleotide
sequence in i)-v), or vii) a nucleotide sequence which hybridizes
under stringent conditions with the nucleotide sequence in
i)-vi).
16. The vector according to claim 15, which is a cloning vector or
an expression vector.
17. The vector according to claim 16, which comprises in operable
linkage and in the 5'-3' direction, an expression control region
comprising an enhancer/promoter for driving expression of the
nucleic acid fragment defined in claim 13-i), optionally a signal
peptide coding sequence, a nucleotide sequence defined in claim
13-i), and optionally a terminator.
18. The vector according to claim 13, which is selected from the
group consisting of a virus, a bacteriophage, a plasmid, a
minichromosome, and a cosmid.
19. A method for inducing immunity in an animal by administering at
least once an immunogenically effective amount of a nucleic acid,
said nucleic acid comprising: i) a nucleotide sequence encoding a
polypeptide, said polypeptide comprising: a) an amino acid sequence
selected from the group consisting of any one of SEQ ID NOs: 1-30,
or b) an amino acid sequence consisting of at least 20 contiguous
amino acid residues from any one of SEQ ID NOs: 1-30, or c) an
amino acid sequence having a sequence identity of at least 80% with
the amino acid sequence of a), or d) an amino acid sequence having
a sequence identity of at least 80% with the amino acid sequence of
b) or ii) a nucleotide sequence consisting of the amino acid
encoding part of any one of SEQ ID NOs: 30-90, iii) a nucleotide
sequence consisting of at least 15 consecutive nucleotides in the
amino acid encoding part of any one of SEQ ID NOs: 30-90, iv) a
nucleotide sequence having a sequence identity of at least 80% with
the nucleotide sequence in ii), v) a nucleotide sequence having a
sequence identity of at least 80% with the nucleotide sequence in
iii), vi) a nucleotide sequence complementary to the nucleotide
sequence in i)-v), or vii) a nucleotide sequence which hybridizes
under stringent conditions with the nucleotide sequence in i)-vi)
or an expression vector comprising said nucleic acid so as to
induce adaptive immunity against C. jejuni in the animal.
20. The method according to claim 19, wherein the animal is a human
being.
21. The method according to claim 19, wherein the immunity is
effective in reducing the risk of attracting infection with C.
jejuni or is effective in treating or ameliorating infection with
C. jejuni.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] In accordance with 37 C.F.R. 1.76, a claim of priority is
included in an Application Data Sheet filed concurrently herewith.
Accordingly, the present invention claims priority as a
continuation of U.S. patent application Ser. No. 14/351,423
entitled "PEPTIDES DERIVED FROM CAMPYLOBACTER JEJUNI AND THEIR USE
IN VACCINATION", filed Apr. 11, 2014, which is a national stage
filing in accordance with 35 U.S.C. .sctn.371 of PCT/EP2012/070282,
filed Oct. 12, 2012, which claims the benefit of the priority of
Denmark Patent Application No. PA 2011 00789, filed Oct. 12, 2011,
and U.S. Provisional Patent Application No. 61/550,494, filed Oct.
24, 2011, the contents of each are incorporated herein by
reference.
FIELD OF THE INVENTION
[0002] The present invention relates to the field of antimicrobial
prophylaxis and therapy. In particular the present invention
relates to novel proteins and polynucleotides derived from
Campylobacter jejuni. The invention further relates to vectors
comprising the polynucleotides, transformed host organisms
expressing the polynucleotides, antibodies (mono- or polyclonal)
specific for the polypeptides as well as diagnostic, prophylactic
and therapeutic uses and methods. Finally, also methods of
preparation are part of the invention.
BACKGROUND OF THE INVENTION
[0003] C. jejuni is a bacterium commonly associated with poultry,
since it naturally colonises the digestive tract of many bird
species. Contaminated drinking water and unpasteurized milk provide
an efficient means for distribution in human populations.
Contaminated food is a major source of isolated C. jejuni
infections, with incorrectly prepared meat and poultry normally
being the source of the bacteria.
[0004] Infection with C. jejuni usually results in enteritis, which
is characterised by abdominal pain, diarrhea, fever, and malaise.
The symptoms usually persist for between 24 hours and a week, but
may be longer. Diarrhea can vary in severity from loose stools to
bloody stools. The disease is usually self-limiting. However, it
does respond to antibiotics. Severe (accompanying fevers, blood in
stools) or prolonged cases may require ciprofloxacin, erythromycin,
azithromycin or norfloxacin. The drug of choice is usually
erythromycin. About 90% of cases respond to ciprofloxacin
treatment. Fluid and electrolyte replacement may be required for
serious cases.
[0005] The first full-genome sequence of C. jejuni was performed in
2000 (strain NCTC11168) with a circular chromosome of 1,641,481
base pairs.
[0006] As mentioned, C. jejuni infections may successfully be
treated by administration of antibiotics to patients in need
thereof, but that would not prevent acute illness. Further, due to
careless or thoughtless use of powerful antibiotics, many
pathological germs, including C. jejuni become resistant against
antibiotics over time. In particular in hospitals, treatment with
antibiotics can prove inadequate: not only will a C. jejuni
infection be life-threatening for patients that already suffer from
other health problems meaning that treatment with antibiotics may
simply be non-effective within the relevant time-span, but in
addition antibiotic-resistant C. jejuni strains will also withstand
treatment with those antibiotics used as the initial choice in
treatment. There is thus a need to provide alternatives to current
treatment regimens. Also, infection with C. jejuni is associated
with reactive arthritis and Guillain-Barre Syndrome.
[0007] Vaccination is considered to be a very effective method of
preventing infectious diseases in human and veterinary health care.
Vaccination is the administration of immunogenically effective
amounts of antigenic material (the vaccine) to produce immunity to
a disease/disease-causing pathogenic agent. Vaccines have
contributed to the eradication of smallpox, the near eradication of
polio, and the control of a variety of diseases, including rubella,
measles, mumps, chickenpox, typhoid fever.
[0008] Before "the genomic era", vaccines were based on killed or
live attenuated, microorganisms, or parts purified from them.
Subunit vaccines are considered as a modern upgrade of these types
of vaccine, as the subunit vaccines contain one or more protective
antigens, which are more or less the weak spot of the pathogen.
Hence, in order to develop subunit vaccines, it is critical to
identify the proteins, which are important for inducing protection
and to eliminate others.
[0009] An antigen is said to be protective if it is able to induce
protection from subsequent challenge by a disease-causing
infectious agent in an appropriate animal model following
immunization.
[0010] The empirical approach to subunit vaccine development, which
includes several steps, begins with pathogen cultivation, followed
by purification into components, and then testing of antigens for
protection. Apart from being time and labour consuming, this
approach has several limitations that can lead to failure. It is
not possible to develop vaccines using this approach for
microorganisms, which cannot easily be cultured and only allows for
the identification of the antigens, which can be obtained in
sufficient quantities. The empirical approach has a tendency to
focus on the most abundant proteins, which in some cases are not
immuno-protective. In other cases, the antigen expressed during in
vivo infection is not expressed during in vitro cultivation.
Furthermore, antigen discovery by use of the empirical approach
demands an extreme amount of proteins in order to discover the
protective antigens, which are like finding needles in the
haystack. This renders it a very expensive approach, and it limits
the vaccine development around diseases, which is caused by
pathogens with a large genome or disease areas, which perform badly
in a cost-effective perspective.
OBJECT OF THE INVENTION
[0011] It is an object of embodiments of the invention to provide
C. jejuni derived antigenic polypeptides that may serve as
constituents in vaccines against C. jejuni infections and in
diagnosis of C. jejuni infections. It is also an object to provide
nucleic acids, vectors, transformed cells, vaccine compositions,
and other useful means for molecular cloning as well as for therapy
and diagnosis with relevance for C. jejuni.
SUMMARY OF THE INVENTION
[0012] It has been found by the present inventor(s) that C. jejuni,
in particular drug resistant C. jejuni, expresses a number of
hitherto unknown putatively surface exposed proteins which are
candidates as vaccine targets as well as candidates as immunizing
agents for preparation of antibodies that target C. jejuni. One of
these putatively surface exposed antigens (cj0404; SEQ ID NO: 13)
has now been tested for suitability as a vaccine agent and has as
the only candidate among 25 randomly isolated C. jejuni proteins
been found to be a capable of providing protection against
challenge infection. The remaining 29 variants are currently being
investigated in a similar setup.
[0013] So, in a first aspect the present invention relates to a
polypeptide comprising a) an amino acid sequence selected from the
group consisting of any one of SEQ ID NOs: 1-30, orb) an amino acid
sequence consisting of at least 5 contiguous amino acid residues
from any one of SEQ ID NOs: 1-30, orc) an amino acid sequence
having a sequence identity of at least 60% with the amino acid
sequence of a), d) an amino acid sequence having a sequence
identity of at least 60% with the amino acid sequence of b), ore)
an assembly of amino acids derived from any one of SEQ ID NOs: 1-30
which has essentially the same 3D conformation as in the protein
from which said assembly is derived so as to constitute a B-cell
epitope, said polypeptide being antigenic in a mammal.
[0014] In another aspect, the invention relates to an isolated
nucleic acid fragment, which comprises i) a nucleotide sequence
encoding a polypeptide of the invention, or ii) a nucleotide
sequence consisting of any one of SEQ ID NOs: 31-90. iii) a
nucleotide sequence consisting of at least 10 consecutive
nucleotides in any one of SEQ ID NOs: 31-90, iv) a nucleotide
sequence having a sequence identity of at least 60% with the
nucleotide sequence in i) or ii), v) a nucleotide sequence having a
sequence identity of at least 60% with the nucleotide sequence in
iii), vi) a nucleotide sequence complementary to the nucleotide
sequence in i)-v), or vii) a nucleotide sequence which hybridizes
under stringent conditions with the nucleotide sequence in
i)-vi).
[0015] In a third aspect, the invention relates to a vector
comprising the nucleic acid of the invention, such as a cloning
vector or an expression vector.
[0016] In fourth aspect, the invention relates to a cell which is
transformed so as to carry the vector of the invention.
[0017] In a fifth aspect, the invention relates to a pharmaceutical
composition comprising a polypeptide of the invention, a nucleic
acid fragment of the invention, a vector of the invention, or a
transformed cell of the invention, and a pharmaceutically
acceptable carrier, vehicle or diluent.
[0018] In a sixth aspect, the invention relates to a method for
inducing immunity in an animal by administering at least once an
immunogenically effective amount of a polypeptide of the invention,
a nucleic acid fragment of the invention, a vector of the
invention, a transformed cell of the invention, or a pharmaceutical
composition of the fifth aspect of the invention so as to induce
adaptive immunity against C. jejuni in the animal.
[0019] In a seventh and eighth aspect, the invention relates to 1)
a polyclonal antibody in which the antibodies specifically bind to
at least one polypeptide of the invention, and which is essentially
free from antibodies binding specifically to other C. jejuni
polypeptides, and to 2) an isolated monoclonal antibody or antibody
analogue which binds specifically to a polypeptide of the
invention. In a related ninth aspect, the invention relates to a
pharmaceutical composition comprising such a polyclonal or
monoclona antibody and a pharmaceutically acceptable carrier,
vehicle or diluent.
[0020] In a 10.sup.th aspect, the invention relates to a method for
prophylaxis, treatment or amelioration of infection with C. jejuni,
in particular infection with multi-resistant C. jejuni, comprising
administering a therapeutically effective amount of an antibody of
the 7.sup.th or 8.sup.th aspect of the invention or a
pharmaceutical composition of the eighth aspect to an individual in
need thereof.
[0021] In an 11.sup.th aspect, the invention relates to a method
for determining, quantitatively or qualitatively, the presence of
C. jejuni, in particular the presence of multi-resistant C. jejuni,
in a sample, the method comprising contacting the sample with an
antibody of aspects 8 or 9 of the invention and detecting the
presence of antibody bound to material in the sample.
[0022] In an 12.sup.th aspect of the invention is provided a method
for determining, quantitatively or qualitatively, the presence of
antibodies specific for C. jejuni, in particular the presence of
antibodies specific for multi-resistant C. jejuni, in a sample, the
method comprising contacting the sample with a polypeptide of the
invention and detecting the presence of antibody that specifically
bind said polypeptide.
[0023] In a 13.sup.th aspect, the invention relates to a method for
determining, quantitatively or qualitatively, the presence of a
nucleic acid characteristic of C. jejuni, in particular the
presence of a nucleic acid characteristic of multi-resistant C.
jejuni, in a sample, the method comprising contacting the sample
with a nucleic acid fragment of the invention and detecting the
presence of nucleic acid in the sample that hybridizes to said
nucleic acid fragment.
[0024] In a 14.sup.th aspect, the invention relates to a method for
the preparation of the polypeptide of the invention,
comprising--culturing a transformed cell of the present invention,
which is capable of expressing the nucleic acid of the invention,
under conditions that facilitate that the transformed cell
expresses the nucleic acid fragment of the invention, which encodes
a polypeptide of the invention, and subsequently recovering said
polypeptide, or--preparing said polypeptide by means of solid or
liquid phase peptide synthesis.
[0025] In a 15.sup.th aspect, the invention relates to a method for
determining whether a substance, such as an antibody, is
potentially useful for treating infection with C. jejuni, the
method comprising contacting the polypeptide of the invention with
the substance and subsequently establishing whether the substance
has at least one of the following characteristics: 1) the ability
to bind specifically to said polypeptide, 2) the ability to compeed
with said polypeptide for specific binding to a ligand/receptor,
and 3) the ability to specifically inactivate said polypeptide.
[0026] Finally, in a 16.sup.th aspect, the invention relates to a
method for determining whether a substance, such as a nucleic acid,
is potentially useful for treating infection with C. jejuni, the
method comprising contacting the substance with the nucleic acid
fragment of claim of the invention and subsequently establishing
whether the substance has either the ability to 1) bind
specifically to the nucleic acid fragment, or 2) bind specifically
to a nucleic acid that hybridizes specifically with the nucleic
acid fragment.
LEGENDS TO THE FIGURE
[0027] FIG. 1: Gels showing the expression of 14 proteins of C.
jejuni genes cloned in E. coli BL21.X: presence of expression
product in BL21 after induction. O: No induction of protein
expression.
[0028] FIG. 2: C. jejuni transformed clones were tested for
antibody recognition in a dot blot assay. Four clones were tested
for expression and antigenicity. The dot blots are showing
reactivity of 4 C. jejuni antigens with antiserea from rabbits
immunized with five different C. jejuni strains from five different
isolates. All clones reacted to all sera.
[0029] FIG. 3: Dot blots showing reactivity of 7 C. jejuni antigens
against human sera.
DETAILED DISCLOSURE OF THE INVENTION
Definitions
[0030] The term "polypeptide" is in the present context intended to
mean both short peptides of from 2 to 10 amino acid residues,
oligopeptides of from 11 to 100 amino acid residues, and
polypeptides of more than 100 amino acid residues. Furthermore, the
term is also intended to include proteins, i.e. functional
biomolecules comprising at least one polypeptide; when comprising
at least two polypeptides, these may form complexes, be covalently
linked, or may be non-covalently linked. The polypeptide (s) in a
protein can be glycosylated and/or lipidated and/or comprise
prosthetic groups.
[0031] The term "subsequence" means any consecutive stretch of at
least 3 amino acids or, when relevant, of at least 3 nucleotides,
derived directly from a naturally occurring amino acid sequence or
nucleic acid sequence, respectively
[0032] The term "amino acid sequence" s the order in which amino
acid residues, connected by peptide bonds, lie in the chain in
peptides and proteins.
[0033] The term "adjuvant" has its usual meaning in the art of
vaccine technology, i.e. a substance or a composition of matter
which is 1) not in itself capable of mounting a specific immune
response against the immunogen of the vaccine, but which is 2)
nevertheless capable of enhancing the immune response against the
immunogen. Or, in other words, vaccination with the adjuvant alone
does not provide an immune response against the immunogen,
vaccination with the immunogen may or may not give rise to an
immune response against the immunogen, but the combined vaccination
with immunogen and adjuvant induces an immune response against the
immunogen which is stronger than that induced by the immunogen
alone.
[0034] "Sequence identity" is in the context of the present
invention determined by comparing 2 optimally aligned sequences of
equal length (e.g. DNA, RNA or amino acid) according to the
following formula: (N.sub.ref-N.sub.dif)100/N.sub.ref, wherein
N.sub.ref is the number of residues in one of the 2 sequences and
N.sub.dif is the number of residues which are non-identical in the
two sequences when they are aligned over their entire lengths and
in the same direction. So, two sequences 5'-ATTCGGAAC-3' and
5'-ATACGGGAC-3' will provide the sequence identity 77,78%
(N.sub.ref=9 and N.sub.dif=2).
[0035] An "assembly of amino acids" means two or more amino acids
bound together by physical or chemical means.
[0036] The "3D conformation" is the 3 dimensional structure of a
biomolecule such as a protein. In monomeric polypeptides/proteins,
the 3D conformation is also termed "the tertiary structure" and
denotes the relative locations in 3 dimensional space of the amino
acid residues forming the polypeptide.
[0037] "An immunogenic carrier" is a molecule or moiety to which an
immunogen or a hapten can be coupled in order to enhance or enable
the elicitation of an immune response against the immunogen/hapten.
Immunogenic carriers are in classical cases relatively large
molecules (such as tetanus toxoid, KLH, diphtheria toxoid etc.)
which can be fused or conjugated to an immunogen/hapten, which is
not sufficiently immunogenic in its own right--typically, the
immunogenic carrier is capable of eliciting a strong T-helper
lymphocyte response against the combined substance constituted by
the immunogen and the immunogenic carrier, and this in turn
provides for improved responses against the immunogen by
B-lymphocytes and cytotoxic lymphocytes. More recently, the large
carrier molecules have to a certain extent been substituted by
so-called promiscuous T-helper epitopes, i.e. shorter peptides that
are recognized by a large fraction of HLA haplotypes in a
population, and which elicit T-helper lymphocyte responses.
[0038] A "T-helper lymphocyte response" is an immune response
elicited on the basis of a peptide, which is able to bind to an MHC
class II molecule (e.g. an HLA class II molecule) in an
antigen-presenting cell and which stimulates T-helper lymphocytes
in an animal species as a consequence of T-cell receptor
recognition of the complex between the peptide and the MHC Class II
molecule prese
[0039] An "immunogen" is a substance of matter which is capable of
inducing an adaptive immune response in a host, whose immune system
is confronted with the immunogen. As such, immunogens are a subset
of the larger genus "antigens", which are substances that can be
recognized specifically by the immune system (e.g. when bound by
antibodies or, alternatively, when fragments of the are antigens
bound to MHC molecules are being recognized by T-cell receptors)
but which are not necessarily capable of inducing immunity--an
antigen is, however, always capable of eliciting immunity, meaning
that a host that has an established memory immunity against the
antigen will mount a specific immune response against the
antigen.
[0040] A "hapten" is a small molecule, which can neither induce or
elicit an immune response, but if conjugated to an immunogenic
carrier, antibodies or TCRs that recognize the hapten can be
induced upon confrontation of the immune system with the hapten
carrier conjugate.
[0041] An "adaptive immune response" is an immune response in
response to confrontation with an antigen or immunogen, where the
immune response is specific for antigenic determinants of the
antigen/immunogen--examples of adaptive immune responses are
induction of antigen specific antibody production or antigen
specific induction/activation of T helper lymphocytes or cytotoxic
lymphocytes.
[0042] A "protective, adaptive immune response" is an
antigen-specific immune response induced in a subject as a reaction
to immunization (artificial or natural) with an antigen, where the
immune response is capable of protecting the subject against
subsequent challenges with the antigen or a pathology-related agent
that includes the antigen. Typically, prophylactic vaccination aims
at establishing a protective adaptive immune response against one
or several pathogens.
[0043] "Stimulation of the immune system" means that a substance or
composition of matter exhibits a general, non-specific
immunostimulatory effect. A number of adjuvants and putative
adjuvants (such as certain cytokines) share the ability to
stimulate the immune system. The result of using an
immunostimulating agent is an increased "alertness" of the immune
system meaning that simultaneous or subsequent immunization with an
immunogen induces a significantly more effective immune response
compared to isolated use of the immunogen.
[0044] Hybridization under "stringent conditions" is herein defined
as hybridization performed under conditions by which a probe will
hybridize to its target sequence, to a detectably greater degree
than to other sequences. Stringent conditions are
target-sequence-dependent and will differ depending on the
structure of the polynucleotide. By controlling the stringency of
the hybridization and/or washing conditions, target sequences can
be identified which are 100% complementary to a probe (homologous
probing). Alternatively, stringency conditions can be adjusted to
allow some mismatching in sequences so that lower degrees of
similarity are detected (heterologous probing). Specificity is
typically the function of post-hybridization washes, the critical
factors being the ionic strength and temperature of the final wash
solution. Generally, stringent wash temperature conditions are
selected to be about 5.degree. C. to about 2.degree. C. lower than
the melting point (Tm) for the specific sequence at a defined ionic
strength and pH. The melting point, or denaturation, of DNA occurs
over a narrow temperature range and represents the disruption of
the double helix into its complementary single strands. The process
is described by the temperature of the midpoint of transition, Tm,
which is also called the melting temperature. Formulas are
available in the art for the determination of melting
temperatures.
[0045] The term "animal" is in the present context in general
intended to denote an animal species (preferably mammalian), such
as Homo sapiens, Canis domesticus, etc. and not just one single
animal. However, the term also denotes a population of such an
animal species, since it is important that the individuals
immunized according to the method of the invention substantially
all will mount an immune response against the immunogen of the
present invention.
[0046] As used herein, the term "antibody" refers to a polypeptide
or group of polypeptides composed of at least one antibody
combining site. An "antibody combining site" is the
three-dimensional binding space with an internal surface shape and
charge distribution complementary to the features of an epitope of
an antigen, which allows a binding of the antibody with the
antigen. "Antibody" includes, for example, vertebrate antibodies,
hybrid antibodies, chimeric antibodies, humanised antibodies,
altered antibodies, univalent antibodies, Fab proteins, and single
domain antibodies.
[0047] "Specific binding" denotes binding between two substances
which goes beyond binding of either substance to randomly chosen
substances and also goes beyond simple association between
substances that tend to aggregate because they share the same
overall hydrophobicity or hydrophilicity. As such, specific binding
usually involves a combination of electrostatic and other
interactions between two conformationally complementary areas on
the two substances, meaning that the substances can "recognize"
each other in a complex mixture.
[0048] The term "vector" is used to refer to a carrier nucleic acid
molecule into which a heterologous nucleic acid sequence can be
inserted for introduction into a cell where it can be replicated
and expressed. The term further denotes certain biological vehicles
useful for the same purpose, e.g. viral vectors and phage--both
these infectious agents are capable of introducing a heterologous
nucleic acid sequence
[0049] The term "expression vector" refers to a vector containing a
nucleic acid sequence coding for at least part of a gene product
capable of being transcribed. In some cases, when the transcription
product is an mRNA molecule, this is in turn translated into a
protein, polypeptide, or peptide.
Specific Embodiments of the Invention
The Polypeptides of the Invention
[0050] In some embodiments the at least 5 contiguous amino acids
referred to in option b) in the definition of the first aspect of
the invention constitute at least 6, such as at least 7, at least
8, at least 9, at least 10, at least 11, at least 12, at least 13,
at least 14, at least 15, at least 16, at least 17, at least 18, at
least 19, at least 20, at least 21, at least 22, at least 23, at
least 24, at least 25, at least 26, at least 27 at least 28, at
least 29, at least 30, at least 31, at least 32, at least 33, at
least 34, at least 35, at least 36, at least 37, at least 38, at
least 39, at least 40, at least 41, at least 42, at least 43, at
least 44, at least 45, at least 46, at least 47, at least 48, at
least 49, at least 50, and at least 51 contiguous amino acid
residues.
[0051] The number may, where applicable, be higher, such as at
least 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,
67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,
84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,
100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,
113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, at least
124, and at least 125 contiguous amino acid residues. Another way
to phrase this is that for each of SEQ ID NOs: 1-30, the number of
the contiguous amino acid residues is at least N-n, where N is the
length of the sequence ID in question and n is any integer between
6 and N-1; that is, the at least 5 contiguous amino acids can be at
least any number between 5 and the length of the reference sequence
minus one, in increments of one.
[0052] In some embodiments, the polypeptide of the invention also
has a sequence identity with the amino acid sequence of a) defined
above of at least 65%, such as at least 70%, at least 75%, at least
80%, at least 85%, at least 90%, at least 91%, at least 92%, at
least 93%, at least 94%, at least 95%, at least 96%, at least 97%,
at least 98%, and at least 99%. Similarly, the polypeptide of the
invention in some embodiments also has a sequence identity with the
amino acid sequence of b) defined above of at least 60%, such as at
least 65%, at least 70%, at least 75%, at least 80%, at least 85%,
at least 90%, at least 91%, at least 92%, at least 93%, at least
94%, at least 95%, at least 96%, at least 97%, at least 98%, and at
least 99%.
[0053] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,
15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31,
32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, and 47
in any one of SEQ ID NOs: 1-30, if the length of the at least 5
amino acid residues so permit--if the length of the at least 5
amino acids are higher than 5, the N-terminal first residue will
not be higher numbered than N-L+1, where N is the number of amino
acid residues of the reference sequence and L is the number of
amino acids defined for option b.
[0054] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,
60, and 61 in any on of SEQ ID NOs: 2-30, if the length of the at
least 5 amino acid residues so permit--if the length of the at
least 5 amino acids are higher than 5, the N-terminal first residue
will not be higher numbered than N-L+1, where N is the number of
amino acid residues of the reference sequence and L is the number
of amino acids defined for option b.
[0055] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73,
74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90,
91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,
106, 107, 108, 109, 110, 111, and 112 in any one of SEQ ID NOs:
3-30, if the length of the at least 5 amino acid residues so
permit--if the length of the at least 5 amino acids are higher than
5, the N-terminal first residue will not be higher numbered than
N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0056] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 113, 114, 115, 116, 117, 118, 119, 120, 121,
122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, and 134
in any one of SEQ ID NOs: 4-30, if the length of the at least 5
amino acid residues so permit--if the length of the at least 5
amino acids are higher than 5, the N-terminal first residue will
not be higher numbered than N-L+1, where N is the number of amino
acid residues of the reference sequence and L is the number of
amino acids defined for option b.
[0057] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 135, 136, and 137 in any one of SEQ ID NOs:
5-30, if the length of the at least 5 amino acid residues so
permit--if the length of the at least 5 amino acids are higher than
5, the N-terminal first residue will not be higher numbered than
N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0058] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 138, 139, and 140 in any one of SEQ ID NOs:
6-30, if the length of the at least 5 amino acid residues so
permit--if the length of the at least 5 amino acids are higher than
5, the N-terminal first residue will not be higher numbered than
N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0059] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 141, 142, 143, 144, 145, 146, 147, 148, 149,
150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,
163, 164, 165, 166, 167, 168, 169, and 170 in any one of SEQ ID
NOs: 7-30, if the length of the at least 5 amino acid residues so
permit--if the length of the at least 5 amino acids are higher than
5, the N-terminal first residue will not be higher numbered than
N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0060] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 171, 172, 173, 174, 175, 176, 177, 178, 179,
180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,
193, 194, 195, 196, and 197 in any one of SEQ ID NOs: 8-30, if the
length of the at least 5 amino acid residues so permit--if the
length of the at least 5 amino acids are higher than 5, the
N-terminal first residue will not be higher numbered than N-L+1,
where N is the number of amino acid residues of the reference
sequence and L is the number of amino acids defined for option
b.
[0061] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 198, 199, 200, 201, 202, 203, 204, 205, and 206
in any one of SEQ ID NOs: 9-30, if the length of the at least 5
amino acid residues so permit--if the length of the at least 5
amino acids are higher than 5, the N-terminal first residue will
not be higher numbered than N-L+1, where N is the number of amino
acid residues of the reference sequence and L is the number of
amino acids defined for option b.
[0062] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 207, 208, 209, 210, 211, 212, 213, 214, 215,
216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228,
229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, and 240 in
any one of SEQ ID NOs: 10-30, if the length of the at least 5 amino
acid residues so permit--if the length of the at least 5 amino
acids are higher than 5, the N-terminal first residue will not be
higher numbered than N-L+1, where N is the number of amino acid
residues of the reference sequence and L is the number of amino
acids defined for option b.
[0063] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 241, 242, 243, 244, 245, 246, 247, 248, 249,
250, 251, 252, 253, 254, and 255 in any one of SEQ ID NOs: 11-30,
if the length of the at least 5 amino acid residues so permit--if
the length of the at least 5 amino acids are higher than 5, the
N-terminal first residue will not be higher numbered than N-L+1,
where N is the number of amino acid residues of the reference
sequence and L is the number of amino acids defined for option
b.
[0064] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 256, 257, 258, 259, 260, 261, 262, 263, 264,
265, 266, 267, 268, and 269 in any one of SEQ ID NOs: 12-30, if the
length of the at least 5 amino acid residues so permit--if the
length of the at least 5 amino acids are higher than 5, the
N-terminal first residue will not be higher numbered than N-L+1,
where N is the number of amino acid residues of the reference
sequence and L is the number of amino acids defined for option
b.
[0065] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 270, 271, 272, 273, and 274 in any one of SEQ
ID NOs: 13-30, if the length of the at least 5 amino acid residues
so permit--if the length of the at least 5 amino acids are higher
than 5, the N-terminal first residue will not be higher numbered
than N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0066] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 275, 276, 277, 278, 279, 280, 281, 282, 283,
284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,
297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,
310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,
323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,
336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,
349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,
362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,
375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, and 386 in
any one of SEQ ID NOs: 14-30, if the length of the at least 5 amino
acid residues so permit--if the length of the at least 5 amino
acids are higher than 5, the N-terminal first residue will not be
higher numbered than N-L+1, where N is the number of amino acid
residues of the reference sequence and L is the number of amino
acids defined for option b.
[0067] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 387, 388, 389, 390, 391, 392, 393, 394, 395,
396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,
409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,
422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,
435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447,
448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460,
461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473,
474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486,
487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499,
500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512,
513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525,
526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, and 538
in any one of SEQ ID NOs: 15-30, if the length of the at least 5
amino acid residues so permit--if the length of the at least 5
amino acids are higher than 5, the N-terminal first residue will
not be higher numbered than N-L+1, where N is the number of amino
acid residues of the reference sequence and L is the number of
amino acids defined for option b.
[0068] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 539, 540, 541, 542, 543, 544, 545, 546, 547,
548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560,
561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573,
574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586,
587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599,
600, 601, 602, 603, 604, 605, and 606 in any one of SEQ ID NOs:
16-30, if the length of the at least 5 amino acid residues so
permit--if the length of the at least 5 amino acids are higher than
5, the N-terminal first residue will not be higher numbered than
N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0069] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 607, 608, 609, 610, 611, 612, 613, 614, 615,
616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628,
629, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641,
642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654,
655 in any one of SEQ ID NOs: 17-30, if the length of the at least
5 amino acid residues so permit--if the length of the at least 5
amino acids are higher than 5, the N-terminal first residue will
not be higher numbered than N-L+1, where N is the number of amino
acid residues of the reference sequence and L is the number of
amino acids defined for option b.
[0070] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 656, 657, 658, 659, 660, 661 in any one of SEQ
ID NOs: 18-30, if the length of the at least 5 amino acid residues
so permit--if the length of the at least 5 amino acids are higher
than 5, the N-terminal first residue will not be higher numbered
than N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0071] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 662, 663, 664, 665, 666, 667, 668, 669, 670,
671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683,
684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696,
697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709,
710, 711, 712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722,
723, 724, 725, 726, 727, 728, 729, 730, 731, 732, 733, 734, 735,
736, 737, 738, 739, 740, 741, 742, 743, 744, 745, and 746 in any
one of SEQ ID NOs: 19-30, if the length of the at least 5 amino
acid residues so permit--if the length of the at least 5 amino
acids are higher than 5, the N-terminal first residue will not be
higher numbered than N-L+1, where N is the number of amino acid
residues of the reference sequence and L is the number of amino
acids defined for option b.
[0072] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 747, 748, 749, 750, 751, 752, 753, 754, 755,
756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767, 768,
769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, 781,
782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794,
795, 796, 797, 798, 799, 800, 801, 802, 803, 804, 805, 806, 807,
808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818, 819, 820,
821, 822, 823, 824, 825, 826, 827, 828, 829, 830, 831, 832, 833,
834, 835, 836, 837, 838, 839, 840, 841, 842, 843, 844, 845, 846,
847, 848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859,
860, and 861 in any one of SEQ ID NOs: 20-30, if the length of the
at least 5 amino acid residues so permit--if the length of the at
least 5 amino acids are higher than 5, the N-terminal first residue
will not be higher numbered than N-L+1, where N is the number of
amino acid residues of the reference sequence and L is the number
of amino acids defined for option b.
[0073] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 862, 863, 864, 865, 866, and 867 in any one of
SEQ ID NOs: 21-30, if the length of the at least 5 amino acid
residues so permit--if the length of the at least 5 amino acids are
higher than 5, the N-terminal first residue will not be higher
numbered than N-L+1, where N is the number of amino acid residues
of the reference sequence and L is the number of amino acids
defined for option b.
[0074] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 868, 869, 870, 871, 872, 873, 874, 875, 876,
877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,
890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902,
903, 904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915,
916, 917, 918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928,
929, 930, 931, 932, 933, 934, 935, 936, 937, 938, 939, 940, 941,
and 942 in any one of SEQ ID NOs: 22-30, if the length of the at
least 5 amino acid residues so permit--if the length of the at
least 5 amino acids are higher than 5, the N-terminal first residue
will not be higher numbered than N-L+1, where N is the number of
amino acid residues of the reference sequence and L is the number
of amino acids defined for option b.
[0075] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 943, 944, 945, 946, 947, 948, 949, 950, 951,
952, 953, 954, 955, 956, 957, 958, 959, 960, 961, 962, 963, 964,
965, 966, 967, 968, 969, 970, 971, 972, 973, 974, 975, 976, 977,
978, 979, 980, 981, 982, 983, 984, 985, 986, 987, 988, 989, 990,
991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001, 1002,
1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,
1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024,
1025, 1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035,
1036, and 1037 in any one of SEQ ID NOs: 23-30, if the length of
the at least 5 amino acid residues so permit--if the length of the
at least 5 amino acids are higher than 5, the N-terminal first
residue will not be higher numbered than N-L+1, where N is the
number of amino acid residues of the reference sequence and L is
the number of amino acids defined for option b.
[0076] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1038, 1039, 1040, 1041, 1042, 1043, 1044, 1045,
1046, 1047, 1048, 1049, 1050, 1051, 1052, 1053, 1054, 1055, 1056,
1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067,
1068, 1069, 1070, 1071, 1072, 1073, 1074, 1075, 1076, 1077, 1078,
1079, 1080, 1081, 1082, 1083, 1084, and 1085 in any one of SEQ ID
NOs: 24-30, if the length of the at least 5 amino acid residues so
permit--if the length of the at least 5 amino acids are higher than
5, the N-terminal first residue will not be higher numbered than
N-L+1, where N is the number of amino acid residues of the
reference sequence and L is the number of amino acids defined for
option b.
[0077] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1086, 1087, 1088, 1089, 1090, 1091, 1092, 1093,
1094, 1095, 1096, 1097, 1098, 1099, 1100, 1101, 1102, 1103, 1104,
1105, 1106, 1107, 1108, 1109, 1110, 1111, 1112, 1113, 1114, 1115,
and 1116 in any one of SEQ ID NOs: 25-30, if the length of the at
least 5 amino acid residues so permit--if the length of the at
least 5 amino acids are higher than 5, the N-terminal first residue
will not be higher numbered than N-L+1, where N is the number of
amino acid residues of the reference sequence and L is the number
of amino acids defined for option b.
[0078] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1117, 1118, 1119, 1120, 1121, 1122, 1123, 1124,
1125, 1126, 1127, 1128, 1129, 1130, 1131, 1132, 1133, 1134, 1135,
1136, 1137, 1138, 1139, and 1140 in any one of SEQ ID NOs: 26-30,
if the length of the at least 5 amino acid residues so permit--if
the length of the at least 5 amino acids are higher than 5, the
N-terminal first residue will not be higher numbered than N-L+1,
where N is the number of amino acid residues of the reference
sequence and L is the number of amino acids defined for option
b.
[0079] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1141, 1142, 1143, 1144, 1145, 1146, 1147, 1148,
1149, 1150, 1151, 1152, 1153, 1154, 1155, 1156, 1157, 1158, 1159,
1160, 1161, 1162, 1163, 1164, 1165, 1166, 1167, 1168, 1169, 1170,
1171, 1172, 1173, 1174, 1175, 1176, 1177, 1178, 1179, 1180, 1181,
and 1182 in any one of SEQ ID NOs: 27-30, if the length of the at
least 5 amino acid residues so permit--if the length of the at
least 5 amino acids are higher than 5, the N-terminal first residue
will not be higher numbered than N-L+1, where N is the number of
amino acid residues of the reference sequence and L is the number
of amino acids defined for option b.
[0080] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1183, 1184, 1185, 1186, 1187, 1188, 1189, 1190,
1191, 1192, 1193, 1194, 1195, 1196, 1197, 1198, 1199, 1200, 1201,
1202, 1203, 1204, 1205, 1206, 1207, 1208, 1209, 1210, 1211, 1212,
1213, 1214, 1215, 1216, 1217, 1218, 1219, 1220, 1221, 1222, 1223,
1224, 1225, 1226, 1227, 1228, 1229, 1230, 1231, 1232, 1233, 1234,
1235, 1236, 1237, 1238, 1239, 1240, 1241, 1242, 1243, 1244, 1245,
1246, 1247, 1248, 1249, 1250, 1251, 1252, 1253, 1254, 1255, 1256,
1257, 1258, 1259, 1260, 1261, 1262, 1263, 1264, 1265, 1266, 1267,
1268, 1269, 1270, 1271, 1272, 1273, 1274, 1275, 1276, 1277, 1278,
1279, 1280, 1281, 1282, 1283, 1284, 1285, 1286, 1287, 1288, 1289,
1290, 1291, 1292, 1293, 1294, 1295, 1296, 1297, 1298, 1299, 1300,
1301, 1302, 1303, 1304, 1305, 1306, 1307, 1308, 1309, 1310, 1311,
1312, 1313, 1314, 1315, 1316, 1317, 1318, 1319, 1320, 1321, 1322,
1323, 1324, 1325, 1326, 1327, 1328, 1329, 1330, 1331, 1332, 1333,
1334, 1335, 1336, 1337, 1338, 1339, 1340, 1341, 1342, 1343, 1344,
1345, 1346, 1347, 1348, 1349, 1350, 1351, 1352, 1353, 1354, 1355,
1356, 1357, 1358, 1359, 1360, 1361, 1362, 1363, 1364, 1365, 1366,
1367, 1368, 1369, 1370, 1371, 1372, 1373, and 1374 in any one of
SEQ ID NOs: 28-30, if the length of the at least 5 amino acid
residues so permit--if the length of the at least 5 amino acids are
higher than 5, the N-terminal first residue will not be higher
numbered than N-L+1, where N is the number of amino acid residues
of the reference sequence and L is the number of amino acids
defined for option b.
[0081] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1375, 1376, 1377, 1378, 1379, 1380, 1381, 1382,
1383, 1384, 1385, 1386, 1387, 1388, 1389, 1390, 1391, 1392, 1393,
1394, 1395, 1396, 1397, 1398, 1399, 1400, 1401, 1402, 1403, 1404,
1405, 1406, 1407, 1408, 1409, 1410, 1411, 1412, 1413, 1414, 1415,
1416, 1417, 1418, 1419, 1420, 1421, 1422, 1423, 1424, 1425, 1426,
1427, 1428, 1429, 1430, 1431, 1432, 1433, 1434, 1435, 1436, 1437,
1438, 1439, 1440, 1441, 1442, 1443, 1444, 1445, 1446, 1447, 1448,
1449, 1450, 1451, 1452, 1453, 1454, 1455, 1456, 1457, 1458, 1459,
1460, 1461, 1462, 1463, 1464, 1465, 1466, 1467, 1468, 1469, 1470,
1471, 1472, 1473, 1474, 1475, 1476, 1477, 1478, 1479, 1480, 1481,
1482, 1483, 1484, 1485, 1486, 1487, 1488, 1489, 1490, 1491, and
1492 in SEQ ID NO: 29 or 30, if the length of the at least 5 amino
acid residues so permit--if the length of the at least 5 amino
acids are higher than 5, the N-terminal first residue will not be
higher numbered than N-L+1, where N is the number of amino acid
residues of the reference sequence and L is the number of amino
acids defined for option b.
[0082] In the embodiments defined by option b) above, the
polypeptide of the invention is also one that has at least 5
contiguous amino acid residues defined for option b) above and also
has its N-terminal amino acid residue corresponding to any one of
amino acid residues 1493, 1494, 1495, 1496, 1497, 1498, 1499, 1500,
1501, 1502, 1503, 1504, 1505, 1506, 1507, 1508, 1509, 1510, 1511,
1512, 1513 in SEQ ID NO: 30, if the length of the at least 5 amino
acid residues so permit--if the length of the at least 5 amino
acids are higher than 5, the N-terminal first residue will not be
higher numbered than N-L+1, where N is the number of amino acid
residues of the reference sequence and L is the number of amino
acids defined for option b.
[0083] The polypeptide of the invention is in certain embodiments
also fused or conjugated to an immunogenic carrier molecule; or,
phrased otherwise, the polypeptide of the invention also includes
such an immunogenic carrier molecule in addition to the material
derived from SEQ ID NOs. 1-30. The immunogenic carrier molecule is
a typically polypeptide that induces T-helper lymphocyte responses
in a majority of humans, such as immunogenic carrier proteins
selected from the group consisting of keyhole limpet hemocyanino or
a fragment thereof, tetanus toxoid or a fragment thereof,
diphtheria toxoid or a fragment thereof. Other suitable carrier
molecules are discussed infra.
[0084] In preferred embodiments, the polypeptide of the invention
detailed above is capable of inducing an adaptive immune response
against the polypeptide in a mammal, in particular in a human
being. Preferably, the adaptive immune response is a protective
adaptive immune response against infection with C. jejuni, in
particular multi-resistant C. jejuni. The polypeptide may in these
cases induce a humeral and/or a cellular immune response.
Epitopes
[0085] SEQ ID NOs: 1-30 include antigenic determinants (epitopes)
that are as such recognized by antibodies and/or when bound to MHC
molecules by T-cell receptors. For the purposes of the present
invention, B-cell epitopes (i.e. antibody binding epitopes) are of
particular relevance.
[0086] It is relatively uncomplicated to identify linear B-cell
epitopes--one very simple approach entails that antibodies raised
agains C. jejuni or C. jejuni derived proteins disclosed herein are
tested for binding to overlapping oligomeric peptides derived from
any one of SEQ ID NO: 1-30. Thereby, the regions of the C. jejuni
polypeptide which are responsible for or contribute to binding to
the antibodies can be identified.
[0087] Alternatively, or additionally, one can produce mutated
versions of the polypeptides of the invention, e.g. version where
each single non-alanine residue in SEQ ID NOs.: 1-30 are point
mutated to alanine--this method also assists in identifying complex
assembled B-cell epitopes; this is the case when binding of the
same antibody is modified by exchanging amino acids in different
areas of the full-length polypeptide.
[0088] Also, in silico methods for B-cell epitope prediction can be
employed: useful state-of-the-art systems for .beta.-turn
prediction is provided in Petersen B et al. (November 2010), Plos
One 5(11): e15079; prediction of linear B-cell epitopes, cf: Larsen
J E P et al. (April 2006), Immunome Research, 2:2; prediction of
solvent exposed amino acids: Petersen B et al (July 2009), BMC
Structural Biology, 9:51.
The Nucleic Acid Fragments of the Invention
[0089] The nucleic acid fragment of the invention referred to above
is preferably is a DNA fragment (such as SEQ ID NOs: 31-60) or an
RNA fragment (such as SEQ ID NOs 61-90).
[0090] The nucleic acid fragment of the invention typically
consists of at least 11, such as at least 12, at least 13, at least
14, at least 15, at least 16, at least 17 at least 18, at least 19,
at least 20, at least 21, at least 22, at least 23, at least 24, at
least 25, at least 26, at least 27, at least 28, at least 29, at
least 30, at least 31, at least 32, at least 33, at least 34, at
least 35, at least 36, at least 37, at least 38, at least 39, at
least 40, at least 41, at least 42, at least 43, at least 44, at
least 45, at least 46, at least 47, at least 48, at least 49, at
least 50, at least 51, at least 52, at least 53, at least 54, at
least 55, at least 56, at least 57, at least 58, at least 59, at
least 60, at least 61, at least 62, at least 63, at least 64, at
least 65, at least 66, at least 67, at least 68, at least 69, at
least 70, at least 71, at least 72, at least 73, at least 74, at
least 75, at least 76, at least 77, at least 78, at least 79, at
least 80, at least 81, at least 82, at least 83, at least 84, at
least 85, at least 86, at least 87, at least 88, at least 89, at
least 90, at least 91, at least 92, at least 93, at least 94, at
least 95, at least 96, at least 97, at least 98, at least 99, at
least 100, at least 101, at least 102, at least 103, at least 104,
at least 105, at least 106, at least 107, at least 108, at least
109, at least 110, at least 111, at least 112, at least 113, at
least 114, at least 115, at least 116, at least 117, at least 118,
at least 119, at least 120, at least 121, at least 122, at least
123, at least 124, at least 125, at least 126, at least 127, at
least 128, at least 129, at least 130, at least 131, at least 132,
at least 133, at least 134, at least 135, at least 136, at least
137, at least 138, at least 139, at least 140, at least 141, at
least 142, at least 143, at least 144, at least 145, at least 146,
at least 147, at least 148, at least 149, at least 150, at least
151, at least 152, and at least 153 consecutive nucleotides in any
one of SEQ ID NOs: 31-90. Longer fragments are contemplated, i.e.
fragments having at least 200, at least 300 at least 400, at least
500, at least 600, at least 700, at least 800, at least 900, at
least 1000, at least 1500, at least 2000, at least 2500, at least
3000, at least 3500, and at least 4000 nucleotides from those of
SEQ ID NOs: 31-90 that encompass fragments of such lengths.
[0091] The nucleic acid fragment of the invention discussed above
typically has a sequence identity with the nucleotide sequence
defined for i) or ii) above, which is at least 65%, such as at
least 70%, at least 75%, at least 80%, at least 85%, at least 90%,
at least 91%, at least 92%, at least 93%, at least 94%, at least
95%, at least 96%, at least 97%, at least 98%, and at least
99%.
[0092] The nucleic acid fragment of the invention discussed above
may also have a sequence identity with the nucleotide sequence
defined for iii) above, which is at least 65%, such as at least
70%, at least 75%, at least 80%, at least 85%, at least 90%, at
least 91%, at least 92%, at least 93%, at least 94%, at least 95%,
at least 96%, at least 97%, at least 98%, and at least 99%.
The Vectors of the Invention
[0093] Vectors of the invention fall into several categories
discussed infra. One preferred vector of the invention comprises in
operable linkage and in the 5'-3' direction, an expression control
region comprising an enhancer/promoter for driving expression of
the nucleic acid fragment defined for option i) above, optionally a
signal peptide coding sequence, a nucleotide sequence defined for
option i), and optionally a terminator. Hence, such a vector
constitutes an expression vector useful for effecting production in
cells of the polypeptide of the invention. Since the polypeptides
of the invention are bacterial of origin, recombinant production is
conveniently effected in bacterial host cells, so here it is
preferred that the expression control region drives expression in
prokaryotic cell such as a bacterium, e.g. in E coli. However, if
the vector is to drive expression in mammalian cell (as would be
the case for a DNA vaccine vector), the expression control region
should be adapted to this particular use.
[0094] At any rate, certain vectors of the invention are capable of
autonomous replication.
[0095] Also, the vector of the invention may be one that is capable
of being integrated into the genome of a host cell--this is
particularly useful if the vector is use in the production of
stably transformed cells, where the progeny will also include the
genetic information introduced via the vector. Alternatively,
vectors incapable of being integrated into the genome of a
mammalian host cell are useful in e.g. DNA vaccination.
[0096] Typically, the vector of the invention is selected from the
group consisting of a virus, such as a attenuated virus (which may
in itself be useful as a vaccine agent), a bacteriophage, a
plasmid, a minichromosome, and a cosmid.
[0097] A more detailed discussion of vectors of the invention is
provided in the following:
Polypeptides of the invention may be encoded by a nucleic acid
molecule comprised in a vector. A nucleic acid sequence can be
"heterologous," which means that it is in a context foreign to the
cell in which the vector is being introduced, which includes a
sequence homologous to a sequence in the cell but in a position
within the host cell where it is ordinarily not found. Vectors
include naked DNAs, RNAs, plasmids, cosmids, viruses
(bacteriophage, animal viruses, and plant viruses), and artificial
chromosomes (e.g., YACs). One of skill in the art would be well
equipped to construct a vector through standard recombinant
techniques (for example Sambrook et al, 2001; Ausubel et al, 1996,
both incorporated herein by reference). In addition to encoding the
polypeptides of this invention, a vector of the present invention
may encode polypeptide sequences such as a tag or immunogenicity
enhancing peptide (e.g. an immunogenic carrier or a fusion partner
that stimulates the immune system, such as a cytokine or active
fragment thereof). Useful vectors encoding such fusion proteins
include pIN vectors (Inouye et al, 1985), vectors encoding a
stretch of histidines, and pGEX vectors, for use in generating
glutathione S-transferase (GST) soluble fusion proteins for later
purification and separation or cleavage.
[0098] Vectors of the invention may be used in a host cell to
produce a polypeptide of the invention that may subsequently be
purified for administration to a subject or the vector may be
purified for direct administration to a subject for expression of
the protein in the subject (as is the case when administering a
nucleic acid vaccine).
[0099] Expression vectors can contain a variety of "control
sequences," which refer to nucleic acid sequences necessary for the
transcription and possibly translation of an operably linked coding
sequence in a particular host organism. In addition to control
sequences that govern transcription and translation, vectors and
expression vectors may contain nucleic acid sequences that serve
other functions as well and are described infra.
1. Promoters and Enhancers
[0100] A "promoter" is a control sequence. The promoter is
typically a region of a nucleic acid sequence at which initiation
and rate of transcription are controlled. It may contain genetic
elements at which regulatory proteins and molecules may bind such
as RNA polymerase and other transcription factors. The phrases
"operatively positioned," "operatively linked," "under control,"
and "under transcriptional control" mean that a promoter is in a
correct functional location and/or orientation in relation to a
nucleic acid sequence to control transcriptional initiation and
expression of that sequence. A promoter may or may not be used in
conjunction with an "enhancer," which refers to a cis-acting
regulatory sequence involved in the transcriptional activation of a
nucleic acid sequence.
[0101] A promoter may be one naturally associated with a gene or
sequence, as may be obtained by isolating the 5' non-coding
sequences located upstream of the coding segment or exon. Such a
promoter can be referred to as "endogenous." Similarly, an enhancer
may be one naturally associated with a nucleic acid sequence,
located either downstream or upstream of that sequence.
Alternatively, certain advantages will be gained by positioning the
coding nucleic acid segment under the control of a recombinant or
heterologous promoter, which refers to a promoter that is not
normally associated with a nucleic acid sequence in its natural
environment. A recombinant or heterologous enhancer refers also to
an enhancer not normally associated with a nucleic acid sequence in
its natural state. Such promoters or enhancers may include
promoters or enhancers of other genes, and promoters or enhancers
isolated from any other prokaryotic, viral, or eukaryotic cell, and
promoters or enhancers not "naturally occurring," i.e., containing
different elements of different transcriptional regulatory regions,
and/or mutations that alter expression. In addition to producing
nucleic acid sequences of promoters and enhancers synthetically,
sequences may be produced using recombinant cloning and/or nucleic
acid amplification technology, including PCR.TM., in connection
with the compositions disclosed herein (see U.S. Pat. No.
4,683,202, U.S. Pat. No. 5,928,906, each incorporated herein by
reference).
[0102] Naturally, it may be important to employ a promoter and/or
enhancer that effectively direct(s) the expression of the DNA
segment in the cell type or organism chosen for expression. Those
of skill in the art of molecular biology generally know the use of
promoters, enhancers, and cell type combinations for protein
expression (see Sambrook et al, 2001, incorporated herein by
reference). The promoters employed may be constitutive,
tissue-specific, or inducible and in certain embodiments may direct
high level expression of the introduced DNA segment under specified
conditions, such as large-scale production of recombinant proteins
or peptides.
[0103] Examples of inducible elements, which are regions of a
nucleic acid sequence that can be activated in response to a
specific stimulus, include but are not limited to Immunoglobulin
Heavy Chain (Banerji et al, 1983; Gilles et al, 1983; Grosschedl et
al, 1985; Atchinson et al, 1986, 1987; toiler et al, 1987;
Weinberger et al, 1984; Kiledjian et al, 1988; Porton et al; 1990),
Immunoglobulin Light Chain (Queen et al, 1983; Picard et al, 1984),
T Cell Receptor (Luria et al, 1987; Winoto et al, 1989; Redondo et
al; 1990), HLA DQa and/or DQp (Sullivan et al, 1987),
.beta.-Interferon (Goodbourn et al, 1986; Fujita et al, 1987;
Goodbourn et al, 1988), Interleukin-2 (Greene et al, 1989),
Interleukin-2 Receptor (Greene et al, 1989; Lin et al, 1990), MHC
Class II 5 (Koch et al, 1989), MHC Class II HLA-DRa (Sherman et al,
1989), .beta.-Actin (Kawamoto et al, 1988; Ng et al; 1989), Muscle
Creatine Kinase (MCK) (Jaynes et al, 1988; Horlick et al, 1989;
Johnson et al, 1989), Prealbumin (Transthyretin) (Costa et al,
1988), Elastase I (Omitz et al, 1987), Metallothionein (MTII)
(Karin et al, 1987; Culotta et al, 1989), Collagenase (Pinkert et
al, 1987; Angel et al, 1987), Albumin (Pinkert et al, 1987; Tranche
et al, 1989, 1990), .alpha.-Fetoprotein (Godbout et al, 1988;
Campere et al, 1989), .gamma.-Globin (Bodine et al, 1987;
Perez-Stable et al, 1990), .beta.-Globin (Trudel et al, 1987),
c-fos (Cohen et al, 1987), c-HA-ras (Triesman, 1986; Deschamps et
al, 1985), Insulin (Edlund et al, 1985), Neural Cell Adhesion
Molecule (NCAM) (Hirsh et al, 1990), al-Antitrypain (Larimer et al,
1990), H2B (TH2B) Histone (Hwang et al, 1990), Mouse and/or Type I
Collagen (Ripe et al, 1989), Glucose-Regulated Proteins (GRP94 and
GRP78) (Chang et al, 1989), Rat Growth Hormone (Larsen et al,
1986), Human Serum Amyloid A (SAA) (Edbrooke et al, 1989), Troponin
I (TN I) (Yutzey et al, 1989), Platelet-Derived Growth Factor
(PDGF) (Peck et al, 1989), Duchenne Muscular Dystrophy (Klamut et
al, 1990), SV40 (Banerji et al, 1981; Moreau et al, 1981; Sleigh et
al, 1985; Firak et al, 1986; Herr et al, 1986; Imbra et al, 1986;
Kadesch et al, 1986; Wang et al, 1986; Ondek et al, 1987; Kuhl et
al, 1987; Schaffner et al, 1988), Polyoma (Swartzendruber et al,
1975; Vasseur at al, 1980; Katinka et al, 1980, 1981; Tyndell et
al, 1981; Dandolo et al, 1983; de Villiers et al, 1984; Hen et al,
1986; Satake et al, 1988; Campbell et al, 1988), Retroviruses
(Kriegler et al, 1982, 1983; Levinson et al, 1982; Kriegler et al,
1983, 1984a, b, 1988; Bosze et al, 1986; Miksicek et al, 1986;
Celander et al, 1987; Thiesen et al, 1988; Celander et al, 1988;
Choi et al, 1988; Reisman et al, 1989), Papilloma Virus (Campo et
al, 1983; Lusky et al, 1983; Spandidos and Wilkie, 1983; Spalholz
et al, 1985; Lusky et al, 1986; Cripe et al, 1987; Gloss et al,
1987; Hirochika et al, 1987; Stephens et al, 1987), Hepatitis B
Virus (Bulla et al, 1986; Jameel et al, 1986; Shaul et al, 1987;
Spandau et al, 1988; Vannice et al, 1988), Human Immunodeficiency
Virus (Muesing et al, 1987; Hauber et al, 1988; Jakobovits et al,
1988; Feng et al, 1988; Takebe et al, 1988; Rosen et al, 1988;
Berkhout et al, 1989; Laspia et al, 1989; Sharp et al, 1989;
Braddock et al, 1989), Cytomegalovirus (CMV) IE (Weber et al, 1984;
Boshart et al, 1985; Foecking et al, 1986), Gibbon Ape Leukemia
Virus (Holbrook et al, 1987; Quinn et al, 1989).
[0104] Inducible Elements include, but are not limited to MT
II-Phorbol Ester (TFA)/Heavy metals (Palmiter et al, 1982;
Haslinger et al, 1985; Searle et al, 1985; Stuart et al, 1985;
Imagawa et al, 1987, Karin et al, 1987; Angel et al, 1987b; McNeall
et al, 1989); MMTV (mouse mammary tumor virus)-Glucocorticoids
(Huang et al, 1981; Lee et al, 1981; Majors et al, 1983; Chandler
et al, 1983; Lee et al, 1984; Ponta et al, 1985; Sakai et al,
1988); .beta.-Interferon-poly(rl).times./poly(rc) (Tavernier et al,
1983); Adenovirus 5 E2-E1A (Imperiale et al, 1984);
Collagenase-Phorbol Ester (TPA) (Angel et al, 1987a);
Stromelysin-Phorbol Ester (TPA) (Angel et al, 1987b); SV40-Phorbol
Ester (TPA) (Angel et al, 1987b); Murine MX Gene-Interferon,
Newcastle Disease Virus (Hug et al, 1988); GRP78 Gene-A23187
(Resendez et al, 1988); .alpha.-2-Macroglobulin-IL-6 (Kunz et al,
1989); Vimentin-Serum (Rittling et al, 1989); MHC Class I Gene
H-2.kappa.b-Interferon (Blanar et al, 1989); HSP70-E1A/SV40 Large T
Antigen (Taylor et al, 1989, 1990a, 1990b); Proliferin-Phorbol
Ester/TPA (Mordacq et al, 1989); Tumor Necrosis Factor-PMA (Hensel
et al, 1989); and Thyroid Stimulating Hormone.alpha. Gene-Thyroid
Hormone (Chatterjee et al, 1989).
[0105] Also contemplated as useful in the present invention are the
dectin-1 and dectin-2 promoters. Additionally any promoter/enhancer
combination (as per the Eukaryotic Promoter Data Base EPDB) could
also be used to drive expression of structural genes encoding
oligosaccharide processing enzymes, protein folding accessory
proteins, selectable marker proteins or a heterologous protein of
interest.
[0106] The particular promoter that is employed to control the
expression of peptide or protein encoding polynucleotide of the
invention is not believed to be critical, so long as it is capable
of expressing the polynucleotide in a targeted cell, preferably a
bacterial cell. Where a human cell is targeted, it is preferable to
position the polynucleotide coding region adjacent to and under the
control of a promoter that is capable of being expressed in a human
cell. Generally speaking, such a promoter might include either a
bacterial, human or viral promoter.
[0107] In various embodiments, the human cytomegalovirus (CMV)
immediate early gene promoter, the SV40 early promoter, and the
Rous sarcoma virus long terminal repeat can be used to obtain high
level expression of a related polynucleotide to this invention. The
use of other viral or mammalian cellular or bacterial phage
promoters, which are well known in the art, to achieve expression
of polynucleotides is contemplated as well.
[0108] In embodiments in which a vector is administered to a
subject for expression of the protein, it is contemplated that a
desirable promoter for use with the vector is one that is not
down-regulated by cytokines or one that is strong enough that even
if down-regulated, it produces an effective amount of the
protein/polypeptide of the current invention in a subject to elicit
an immune response. Non-limiting examples of these are CMV IE and
RSV LTR. In other embodiments, a promoter that is up-regulated in
the presence of cytokines is employed. The MHC I promoter increases
expression in the presence of IFN-.gamma..
[0109] Tissue specific promoters can be used, particularly if
expression is in cells in which expression of an antigen is
desirable, such as dendritic cells or macrophages. The mammalian
MHC I and MHC II promoters are examples of such tissue-specific
promoters. 2. Initiation Signals and Internal Ribosome Binding
Sites (IRES)
[0110] A specific initiation signal also may be required for
efficient translation of coding sequences. These signals include
the ATG initiation codon or adjacent sequences. Exogenous
translational control signals, including the ATG initiation codon,
may need to be provided. One of ordinary skill in the art would
readily be capable of determining this and providing the necessary
signals. It is well known that the initiation codon must be
"in-frame" with the reading frame of the desired coding sequence to
ensure translation of the entire insert. The exogenous
translational control signals and initiation codons can be either
natural or synthetic and may be operable in bacteria or mammalian
cells. The efficiency of expression may be enhanced by the
inclusion of appropriate transcription enhancer elements.
[0111] In certain embodiments of the invention, the use of internal
ribosome entry sites (IRES) elements are used to create multigene,
or polycistronic, messages. IRES elements are able to bypass the
ribosome scanning model of 5' methylated Cap dependent translation
and begin translation at internal sites (Pelletier and Sonenberg,
1988). IRES elements from two members of the picornavirus family
(polio and encephalomyocarditis) have been described (Pelletier and
Sonenberg, 1988), as well an IRES from a mammalian message (Macejak
and Sarnow, 1991). IRES elements can be linked to heterologous open
reading frames. Multiple open reading frames can be transcribed
together, each separated by an IRES, creating polycistronic
messages. By virtue of the IRES element, each open reading frame is
accessible to ribosomes for efficient translation. Multiple genes
can be efficiently expressed using a single promoter/enhancer to
transcribe a single message (see U.S. Pat. Nos. 5,925,565 and
5,935,819, herein incorporated by reference).
2. Multiple Cloning Sites
[0112] Vectors can include a multiple cloning site (MCS), which is
a nucleic acid region that contains multiple restriction enzyme
sites, any of which can be used in conjunction with standard
recombinant technology to digest the vector. (See Carbonelli et al,
1999, Levenson et al, 1998, and Cocea, 1997, incorporated herein by
reference.) Frequently, a vector is linearized or fragmented using
a restriction enzyme that cuts within the MCS to enable exogenous
sequences to be ligated to the vector. Techniques involving
restriction enzymes and ligation reactions are well known to those
of skill in the art of recombinant technology.
3. Splicing Sites
[0113] Most transcribed eukaryotic RNA molecules will undergo RNA
splicing to remove introns from the primary transcripts. If
relevant in the context of vectors of the present invention,
vectors containing genomic eukaryotic sequences may require donor
and/or acceptor splicing sites to ensure proper processing of the
transcript for protein expression. (See Chandler et al, 1997,
incorporated herein by reference.)
4. Termination Signals
[0114] The vectors or constructs of the present invention will
generally comprise at least one termination signal. A "termination
signal" or "terminator" is comprised of the DNA sequences involved
in specific termination of an RNA transcript by an RNA polymerase.
Thus, in certain embodiments a termination signal that ends the
production of an RNA transcript is contemplated. A terminator may
be necessary in vivo to achieve desirable message levels.
[0115] In eukaryotic systems, the terminator region may also
comprise specific DNA sequences that permit site-specific cleavage
of the new transcript so as to expose a polyadenylation site. This
signals a specialized endogenous polymerase to add a stretch of
about 200 A residues (poly A) to the 3' end of the transcript. RNA
molecules modified with this polyA tail appear to more stable and
are translated more efficiently. Thus, in other embodiments
involving eukaryotes, it is preferred that that terminator
comprises a signal for the cleavage of the RNA, and it is more
preferred that the terminator signal promotes polyadenylation of
the message.
[0116] Terminators contemplated for use in the invention include
any known terminator of transcription described herein or known to
one of ordinary skill in the art, including but not limited to, for
example, the bovine growth hormone terminator or viral termination
sequences, such as the SV40 terminator. In certain embodiments, the
termination signal may be a lack of transcribable or translatable
sequence, such as due to a sequence truncation.
5. Polyadenylation Signals
[0117] In expression, particularly eukaryotic expression (as is
relevant in nucleic acid vaccination), one will typically include a
polyadenylation signal to effect proper polyadenylation of the
transcript. The nature of the polyadenylation signal is not
believed to be crucial to the successful practice of the invention,
and/or any such sequence may be employed. Preferred embodiments
include the SV40 polyadenylation signal and/or the bovine growth
hormone polyadenylation signal, convenient and/or known to function
well in various target cells. Polyadenylation may increase the
stability of the transcript or may facilitate cytoplasmic
transport.
6. Origins of Replication
[0118] In order to propagate a vector in a host cell, it may
contain one or more origins of replication sites (often termed
"on"), which is a specific nucleic acid sequence at which
replication is initiated. Alternatively an autonomously replicating
sequence (ARS) can be employed if the host cell is yeast.
7. Selectable and Screenable Markers
[0119] In certain embodiments of the invention, cells containing a
nucleic acid construct of the present invention may be identified
in vitro or in vivo by encoding a screenable or selectable marker
in the expression vector. When transcribed and translated, a marker
confers an identifiable change to the cell permitting easy
identification of cells containing the expression vector.
Generally, a selectable marker is one that confers a property that
allows for selection. A positive selectable marker is one in which
the presence of the marker allows for its selection, while a
negative selectable marker is one in which its presence prevents
its selection. An example of a positive selectable marker is a drug
resistance marker.
[0120] Usually the inclusion of a drug selection marker aids in the
cloning and identification of transformants, for example, markers
that confer resistance to neomycin, puromycin, hygromycin, DHFR,
GPT, zeocin or histidinol are useful selectable markers. In
addition to markers conferring a phenotype that allows for the
discrimination of transformants based on the implementation of
conditions, other types of markers including screenable markers
such as GFP for colorimetric analysis. Alternatively, screenable
enzymes such as herpes simplex virus thymidine kinase (tk) or
chloramphenicol acetyltransferase (CAT) may be utilized. One of
skill in the art would also know how to employ immunologic markers
that can be used in conjunction with FACS analysis. The marker used
is not believed to be important, so long as it is capable of being
expressed simultaneously with the nucleic acid encoding a protein
of the invention. Further examples of selectable and screenable
markers are well known to one of skill in the art.
The Transformed Cells of the Invention
[0121] Transformed cells of the invention are useful as organisms
for producing the polypeptide of the invention, but also as simple
"containers" of nucleic acids and vectors of the invention.
[0122] Certain transformed cells of the invention are capable of
replicating the nucleic acid fragment defined for option i) of the
second aspect of the invention. Preferred transformed cells of the
invention are capable of expressing the nucleic acid fragment
defined for option i).
[0123] For recombinant production it is convenient, but not a
prerequisite that the transformed cell according is prokaryotic,
such as a bacterium, but generally both prokaryotic cells and
eukaryotic cells may be used.
[0124] Suitable prokaryotic cells are bacterial cells selected from
the group consisting of Escherichia (such as E. coli.), Bacillus,
(e.g. Bacillus subtilis) Salmonella, and Mycobacterium [preferably
non-pathogenic, (e.g. M. bovis BCG)].
[0125] Eukaryotic cells can be in the form of yeasts (such as
Saccharomyces cerevisiae) and protozoans. Alternatively, the
transformed eukaryotic cells are derived from a multicellular
organism such as a fungus, an insect cell, a plant cell, or a
mammalian cell.
[0126] For production purposes, it is advantageous that the
transformed cell of the invention is is stably transformed by
having the nucleic acid defined above for option i) stably
integrated into its genome, and in certain embodiments it is also
preferred that the transformed cell secretes or carries on its
surface the polypeptide of the invention, since this facilitates
recovery of the polypeptides produced. A particular version of this
embodiment is one where the transformed cell is a bacterium and
secretion of the polypeptide of the invention is into the
periplasmic space.
[0127] As noted above, stably transformed cells are
preferred--these inter alia allows that cell lines comprised of
transformed cells as defined herein may be established--such cell
lines are particularly preferred aspects of the invention.
[0128] Further details on cells and cell lines are presented in the
following:
[0129] Suitable cells for recombinant nucleic acid expression of
the nucleic acid fragments of the present invention are prokaryotes
and eukaryotes. Examples of prokaryotic cells include E. coli;
members of the Staphylococcus genus, such as S. epidermidis;
members of the Lactobacillus genus, such as L. plantarum; members
of the Lactococcus genus, such as L. lactis; members of the
Bacillus genus, such as B. subtilis; members of the Corynebacterium
genus such as C. glutamicum; and members of the Pseudomonas genus
such as Ps. fluorescens. Examples of eukaryotic cells include
mammalian cells; insect cells; yeast cells such as members of the
Saccharomyces genus (e.g. S. cerevisiae), members of the Pichia
genus (e.g. P. pastoris), members of the Hansenula genus (e.g. H.
polymorpha), members of the Kluyveromyces genus (e.g. K. lactis or
K. fragilis) and members of the Schizosaccharomyces genus (e.g. S.
pombe).
[0130] Techniques for recombinant gene production, introduction
into a cell, and recombinant gene expression are well known in the
art. Examples of such techniques are provided in references such as
Ausubel, Current Protocols in Molecular Biology, John Wiley,
1987-2002, and Sambrook et al., Molecular Cloning, A Laboratory
Manual, 2 nd Edition, Cold Spring Harbor Laboratory Press,
1989.
[0131] As used herein, the terms "cell," "cell line," and "cell
culture" may be used interchangeably. All of these terms also
include their progeny, which is any and all subsequent generations.
It is understood that all progeny may not be identical due to
deliberate or inadvertent mutations. In the context of expressing a
heterologous nucleic acid sequence, "host cell" refers to a
prokaryotic or eukaryotic cell, and it includes any transformable
organism that is capable of replicating a vector or expressing a
heterologous gene encoded by a vector. A host cell can, and has
been, used as a recipient for vectors or viruses. A host cell may
be "transfected" or "transformed," which refers to a process by
which exogenous nucleic acid, such as a recombinant
protein-encoding sequence, is transferred or introduced into the
host cell. A transformed cell includes the primary subject cell and
its progeny.
[0132] Host cells may be derived from prokaryotes or eukaryotes,
including bacteria, yeast cells, insect cells, and mammalian cells
for replication of the vector or expression of part or all of the
nucleic acid sequence(s). Numerous cell lines and cultures are
available for use as a host cell, and they can be obtained through
the American Type Culture Collection (ATCC), which is an
organization that serves as an archive for living cultures and
genetic materials or from other depository institutions such as
Deutsche Sammlung vor Micrroorganismen and Zellkulturen (DSM). An
appropriate host can be determined by one of skill in the art based
on the vector backbone and the desired result. A plasmid or cosmid,
for example, can be introduced into a prokaryote host cell for
replication of many vectors or expression of encoded proteins.
Bacterial cells used as host cells for vector replication and/or
expression include Staphylococcus strains, DH5.alpha., JMl 09, and
KC8, as well as a number of commercially available bacterial hosts
such as SURE.RTM. Competent Cells and SOLOP ACK.TM. Gold Cells
(STRATAGENE.RTM., La Jolla, Calif.). Alternatively, bacterial cells
such as (E. coli LE392) could be used as host cells for phage
viruses. Appropriate yeast cells include Saccharomyces cerevisiae,
Saccharomyces pombe, and Pichia pastoris.
[0133] Examples of eukaryotic host cells for replication and/or
expression of a vector include HeLa, NIH3T3, Jurkat, 293, Cos, CHO,
Saos, and PC12. Many host cells from various cell types and
organisms are available and would be known to one of skill in the
art. Similarly, a viral vector may be used in conjunction with
either a eukaryotic or prokaryotic host cell, particularly one that
is permissive for replication or expression of the vector.
[0134] Some vectors may employ control sequences that allow it to
be replicated and/or expressed in both prokaryotic and eukaryotic
cells. One of skill in the art would further understand the
conditions under which to incubate all of the above described host
cells to maintain them and to permit replication of a vector. Also
understood and known are techniques and conditions that would allow
large-scale production of vectors, as well as production of the
nucleic acids encoded by vectors and their cognate polypeptides,
proteins, or peptides.
Expression Systems
[0135] Numerous expression systems exist that comprise at least a
part or all of the compositions discussed above. Prokaryote- and/or
eukaryote-based systems can be employed for use with the present
invention to produce nucleic acid sequences, or their cognate
polypeptides, proteins and peptides. Many such systems are
commercially and widely available.
[0136] The insect cell/baculovirus system can produce a high level
of protein expression of a heterologous nucleic acid segment, such
as described in U.S. Pat. Nos. 5,871,986, 4,879,236, both herein
incorporated by reference, and which can be bought, for example,
under the name MAXBACC.RTM. 2.0 from INVITROGEN.RTM. and
BACPACK.TM. Baculovirus expression system from CLONTECH.RTM..
[0137] In addition to the disclosed expression systems of the
invention, other examples of expression systems include
STRATAGENE.RTM.'s COMPLETE CONTROL.TM. Inducible Mammalian
Expression System, which involves a synthetic ecdysone-inducible
receptor, or its pET Expression System, an E. coli expression
system. Another example of an inducible expression system is
available from INVITROGEN.RTM., which carries the T-REX.TM.
(tetracycline-regulated expression) System, an inducible mammalian
expression system that uses the full-length CMV promoter.
INVITROGEN.RTM. also provides a yeast expression system called the
Pichia methanolica Expression System, which is designed for
high-level production of recombinant proteins in the methylotrophic
yeast (Pichia methanolica). One of skill in the art would know how
to express a vector, such as an expression construct, to produce a
nucleic acid sequence or its cognate polypeptide, protein, or
peptide.
Amplification of Nucleic Acids
[0138] Nucleic acids used as a template for amplification may be
isolated from cells, tissues or other samples according to standard
methodologies (Sambrook et al, 2001). In certain embodiments,
analysis is performed on whole cell or tissue homogenates or
biological fluid samples without substantial purification of the
template nucleic acid. The nucleic acid may be genomic DNA or
fractionated or whole cell RNA. Where RNA is used, it may be
desired to first convert the RNA to a complementary DNA.
[0139] The term "primer," as used herein, is meant to encompass any
nucleic acid that is capable of priming the synthesis of a nascent
nucleic acid in a template-dependent process. Typically, primers
are oligonucleotides from ten to twenty and/or thirty base pairs in
length, but longer sequences can be employed. Primers may be
provided in double-stranded and/or single-stranded form, although
the single-stranded form is preferred.
[0140] Pairs of primers designed to selectively hybridize to
nucleic acids corresponding to sequences of genes identified herein
are contacted with the template nucleic acid under conditions that
permit selective hybridization. Depending upon the desired
application, high stringency hybridization conditions may be
selected that will only allow hybridization to sequences that are
completely complementary to the primers. In other embodiments,
hybridization may occur under reduced stringency to allow for
amplification of nucleic acids containing one or more mismatches
with the primer sequences. Once hybridized, the template-primer
complex is contacted with one or more enzymes that facilitate
template-dependent nucleic acid synthesis. Multiple rounds of
amplification, also referred to as "cycles," are conducted until a
sufficient amount of amplification product is produced.
[0141] The amplification product may be detected or quantified. In
certain applications, the detection may be performed by visual
means. Alternatively, the detection may involve indirect
identification of the product via chemiluminescence, radioactive
scintigraphy of incorporated radiolabel or fluorescent label or
even via a system using electrical and/or thermal impulse signals
(Bellus, 1994).
[0142] A number of template dependent processes are available to
amplify the oligonucleotide sequences present in a given template
sample. One of the best known amplification methods is the
polymerase chain reaction (referred to as PCR.TM.) which is
described in detail in U.S. Pat. Nos. 4,683,195, 4,683,202 and
4,800,159, and in Innis et al., 1988, each of which is incorporated
herein by reference in their entirety.
[0143] Alternative methods for amplification of target nucleic acid
sequences that may be used in the practice of the present invention
are disclosed in U.S. Pat. Nos. 5,843,650, 5,846,709, 5,846,783,
5,849,546, 5,849,497, 5,849,547, 5,858,652, 5,866,366, 5,916,776,
5,922,574, 5,928,905, 5,928,906, 5,932,451, 5,935,825, 5,939,291
and 5,942,391, GB Application No. 2 202 328, and in PCT Application
No. PCT/US89/01025, each of which is incorporated herein by
reference in its entirety.
Methods of Gene Transfer
[0144] Suitable methods for nucleic acid delivery to effect
expression of compositions of the present invention are believed to
include virtually any method by which a nucleic acid (e.g., DNA,
including viral and nonviral vectors) can be introduced into a
cell, a tissue or an organism, as described herein or as would be
known to one of ordinary skill in the art. Such methods include,
but are not limited to, direct delivery of DNA such as by injection
(U.S. Pat. Nos. 5,994,624, 5,981,274, 5,945,100, 5,780,448,
5,736,524, 5,702,932, 5,656,610, 5,589,466 and 5,580,859, each
incorporated herein by reference), including microinjection
(Harland and Weintraub, 1985; U.S. Pat. No. 5,789,215, incorporated
herein by reference); by electroporation (U.S. Pat. No. 5,384,253,
incorporated herein by reference); by calcium phosphate
precipitation (Graham and Van Der Eb, 1973; Chen and Okayama, 1987;
Rippe et al., 1990); by using DEAE dextran followed by polyethylene
glycol (Gopal, 1985); by direct sonic loading (Fechheimer et al,
1987); by liposome mediated transfection (Nicolau and Sene, 1982;
Fraley et al, 1979; Nicolau et al, 1987; Wong et al, 1980; Kaneda
et al, 1989; Kato et al, 1991); by microprojectile bombardment (PCT
Application Nos. WO 94/09699 and 95/06128; U.S. Pat. Nos.
5,610,042; 5,322,783 5,563,055, 5,550,318, 5,538,877 and 5,538,880,
and each incorporated herein by reference); by agitation with
silicon carbide fibers (Kaeppler et al, 1990; U.S. Pat. Nos.
5,302,523 and 5,464,765, each incorporated herein by reference); by
Agrobacterium mediated transformation (U.S. Pat. Nos. 5,591,616 and
5,563,055, each incorporated herein by reference); or by PEG
mediated transformation of protoplasts (Omirulleh et al, 1993; U.S.
Pat. Nos. 4,684,611 and 4,952,500, each incorporated herein by
reference); by desiccation/inhibition mediated DNA uptake (Potrykus
et al, 1985). Through the application of techniques such as these,
organelle(s), cell(s), tissue(s) or organism(s) may be stably or
transiently transformed.
The Antibodies of the Invention--and their Production/Isolation
[0145] Antibodies directed against the proteins of the invention
are useful for affinity chromatography, immunoassays, and for
distinguishing/identifying staphylococcus proteins as well as for
passive immunisation and therapy.
[0146] Antibodies to the proteins of the invention, both polyclonal
and monoclonal, may be prepared by conventional methods. In
general, the protein is first used to immunize a suitable animal,
preferably a mouse, rat, rabbit or goat. Rabbits and goats are
preferred for the preparation of polyclonal sera due to the volume
of serum obtainable, and the availability of labeled anti-rabbit
and anti-goat antibodies. Immunization is generally performed by
mixing or emulsifying the protein in saline, preferably in an
adjuvant such as Freund's complete adjuvant, and injecting the
mixture or emulsion parenterally (generally subcutaneously or
intramuscularly). A dose of 50-200 .mu.g/injection is typically
sufficient. Immunization is generally boosted 2-6 weeks later with
one or more injections of the protein in saline, preferably using
Freund's incomplete adjuvant. One may alternatively generate
antibodies by in vitro immunization using methods known in the art,
which for the purposes of this invention is considered equivalent
to in vivo immunization. Polyclonal antiserum is obtained by
bleeding the immunized animal into a glass or plastic container,
incubating the blood at 25 C for one hour, followed by incubating
at 4 C for 2-18 hours. The serum is recovered by centrifugation
(eg. 1,000 g for 10 minutes). About 20-50 ml per bleed may be
obtained from rabbits.
[0147] Monoclonal antibodies are prepared using the standard method
of Kohler & Milstein [Nature (1975) 256: 495-96], or a
modification thereof. Typically, a mouse or rat is immunized as
described above. However, rather than bleeding the animal to
extract serum, the spleen (and optionally several large lymph
nodes) is removed and dissociated into single cells. If desired,
the spleen cells may be screened (after removal of nonspecifically
adherent cells) by applying a cell suspension to a plate or well
coated with the protein antigen. B-cells expressing membrane-bound
immunoglobulin specific for the antigen bind to the plate, and are
not rinsed away with the rest of the suspension. Resulting B-cells,
or all dissociated spleen cells, are then induced to fuse with
myeloma cells to form hybridomas, and are cultured in a selective 1
aedium (elg. hypexanthine, aminopterin, thymidine medium,"HAT").
The resulting hybridomas are plated by limiting dilution, and are
assayed for production of antibodies, which bind specifically to
the immunizing antigen (and which do not bind to unrelated
antigens). The selected MAb-secreting hybridomas are then cultured
either in vitro (eg. in tissue culture bottles or hollow fiber
reactors), or in vivo (as ascites in mice).
[0148] If desired, the antibodies (whether polyclonal or
monoclonal) may be labeled using conventional techniques. Suitable
labels include fluorophores, chromophores, radioactive atoms
(particularly 32p and 125I), electron-dense reagents, enzymes, and
ligands having specific binding partners. Enzymes are typically
detected by their activity. For example, horseradish peroxidase is
usually detected by its ability to convert 3,3',
5,5'-tetramethylbenzidine (TMB) to a blue pigment, quantifiable
with a spectrophotometer. "Specific binding partner" refers to a
protein capable of binding a ligand molecule with high specificity,
as for example in the case of an antigen and a monoclonal antibody
specific therefor. Other specific binding partners include biotin
and avidin or streptavidin, IgG and protein A, and the numerous
receptor-ligand couples known in the art. It should be understood
that the above description is not meant to categorize the various
labels into distinct classes, as the same label may serve in
several different modes. For example, 1151 may serve as a
radioactive label or as an electron-dense reagent. HRP may serve as
enzyme or as antigen for a MAb. Further, one may combine various
labels for desired effect. For example, MAbs and avidin also
require labels in the practice of this invention: thus, one might
label a MAb with biotin, and detect its presence with avidin
labeled with, 125I, or with an anti-biotin MAb labeled with HRP.
Other permutations and possibilities will be readily apparent to
those of ordinary skill in the art, and are considered as
equivalents within the scope of the instant invention.
[0149] According to the invention, the isolated monoclonal antibody
or antibody analogue is preferably a monoclonal antibody selected
from a multi-domain antibody such as a murine antibody, a chimeric
antibody such as a humanized antibody, a fully human antibody, and
single-domain antibody of a llama or a camel, or which is an
antibody analogue selected from a fragment of an antibody such as
an Fab or an F(ab').sub.2, an scFV; cf. also the definition of the
term "antibody" presented above.
Compositions of the Invention; Vaccines
[0150] Pharmaceutical compositions, in particular vaccines,
according to the invention may either be prophylactic (ie. to
prevent infection) or therapeutic (ie, to treat disease after
infection).
[0151] Such vaccines comprise immunising antigen(s), immunogen(s),
polypeptide(s), protein(s) or nucleic acid(s), usually in
combination with "pharmaceutically acceptable carriers", which
include any carrier that does not itself induce the production of
antibodies harmful to the individual receiving the composition.
Suitable carriers are typically large, slowly metabolized
macromolecules such as proteins, polysaccharides, polylactic acids,
polyglycolic acids, polymeric amino acids, amino acid copolymers,
lipid aggregates (such as oil droplets or liposomes), and inactive
virus particles.
[0152] Such carriers are well known to those of ordinary skill in
the art. Additionally, these carriers may function as
immunostimulating agents ("adjuvants"). Furthermore, the antigen or
immunogen may be conjugated to a bacterial toxoid, such as a toxoid
from diphtheria, tetanus, cholera, H. pylori, etc. pathogen, cf.
the description of immunogenic carriers supra.
[0153] The pharmaceutical compositions of the invention thus
typically contain an immunological adjuvant, which is commonly an
aluminium based adjuvant or one of the other adjuvants described in
the following:
[0154] Preferred adjuvants to enhance effectiveness of the
composition include, but are not limited to: (1) aluminum salts
(alum), such as aluminum hydroxide, aluminum phosphate, aluminum
sulfate, etc; (2) oil-in-water emulsion formulations (with or
without other specific immunostimulating agents such as muramyl
peptides (see below) or bacterial cell wall components), such as
for example (a) MF59 (WO 90/14837; Chapter 10 in Vaccine design:
the subunit and adjuvant approach, eds. Powell & Newman, Plenum
Press 1995), containing 5% Squalene, 0.5% Tween 80, and 0.5% Span
85 (optionally containing various amounts of MTP-PE (see below),
although not required) formulated into submicron particles using a
microfluidizer such as Model 110Y microfluidizer (Microfluidics,
Newton, Mass.), (b) SAF, containing 10% Squalane, 0.4% Tween 80, 5%
pluronic-blocked polymer L121, and thr-MDP (see below) either
microfluidized into a submicron emulsion or vortexed to generate a
larger particle size emulsion, and (c) Ribi adjuvant system (RAS),
(Ribi Immunochem, Hamilton, Mont.) containing 2% Squalene, 0.2%
Tween 80, and one or more bacterial cell wall components from the
group consisting of monophosphoryl lipid A (MPL), trehalose
dimycolate (TDM), and cell wall skeleton (CWS), preferably MPL+CWS
(Detox.TM.); (3) saponin adjuvants such as Stimulon.TM. (Cambridge
Bioscience, Worcester, Mass.) may be used or particles generated
therefrom such as ISCOMs (immunostimulating complexes); (4)
Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant
(IFA); (5) cytokines, such as interleukins (eg. IL-1, IL-2, IL-4,
IL-5, IL-6, IL-7, IL-12, etc.), interferons (eg. gamma interferon),
macrophage colony stimulating factor (M-CSF), tumor necrosis factor
(TNF), etc.; and (6) other substances that act as immunostimulating
agents to enhance the effectiveness of the composition. Alum and
MF59.TM. adjuvants are preferred.
[0155] As mentioned above, muramyl peptides include, but are not
limited to, N-acetyl-muramyl-L-threonyl-D-isoglutamine (thr-MDP),
N-acetyl-normuramyl-L-alanyl-D-isoglutamine (nor-MDP),
N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2''-2'-dipalmitoyl-sn--
glycero-3-hydroxyphosphoryloxy)-ethylamine (MTP-PE), etc.
[0156] The immunogenic compositions (eg. the immunising antigen or
immunogen or polypeptide or protein or nucleic acid,
pharmaceutically acceptable carrier, and adjuvant) typically will
contain diluents, such as water, saline, glycerol, ethanol, etc.
Additionally, auxiliary substances, such as wetting or emulsifying
agents, pH buffering substances, and the like, may be present in
such vehicles.
[0157] Typically, the immunogenic compositions are prepared as
injectables, either as liquid solutions or suspensions; solid forms
suitable for solution in, or suspension in, liquid vehicles prior
to injection may also be prepared. The preparation also may be
emulsified or encapsulated in liposomes for enhanced adjuvant
effect, as discussed above under pharmaceutically acceptable
carriers.
[0158] Immunogenic compositions used as vaccines comprise an
immunologically effective amount of the antigenic or immunogenic
polypeptides, as well as any other of the above-mentioned
components, as needed. By "immunologically effective amount", it is
meant that the administration of that amount to an individual,
either in a single dose or as part of a series, is effective for
treatment or prevention. This amount varies depending upon the
health and physical condition of the individual to be treated, the
taxonomic group of individual to be treated (eg. non-human primate,
primate, etc.), the capacity of the individual's immune system to
synthesize antibodies or generally mount an immune response, the
degree of protection desired, the formulation of the vaccine, the
treating doctor's assessment of the medical situation, and other
relevant factors. It is expected that the amount will fall in a
relatively broad range that can be determined through routine
trials. However, for the purposes of protein vaccination, the
amount administered per immunization is typically in the range
between 0.5 .mu.g and 500 mg (however, often not higher than 5,000
.mu.g), and very often in the range between 10 and 200 .mu.g.
[0159] The immunogenic compositions are conventionally administered
parenterally, eg, by injection, either subcutaneously,
intramuscularly, or transdermally/transcutaneously (eg.
WO98/20734). Additional formulations suitable for other modes of
administration include oral and pulmonary formulations,
suppositories, and transdermal applications. In the case of nucleic
acid vaccination, also the intravenous or intraarterial routes may
be applicable.
[0160] Dosage treatment may be a single dose schedule or a multiple
dose schedule. The vaccine may be administered in conjunction with
other immunoregulatory agents.
[0161] As an alternative to protein-based vaccines, DNA vaccination
(also termed nucleic acid vaccination or gene vaccination) may be
used [eg. Robinson & Torres (1997) Seminars in Immunol 9:
271-283; Donnelly et al. (1997) Annu Rev Immunol 15: 617-648; later
herein].
Treatment Methods of the Invention
[0162] The method of the sixth aspect of the invention generally
relates to induction of immunity and as such also entails method
that relate to treatment, prophylaxis and amelioration of
disease.
[0163] When immunization methods entail that a polypeptide of the
invention or a composition comprising such a polypeptide is
administered the animal (e.g. the human) typically receives between
0.5 and 5,000 .mu.g of the polypeptide of the invention per
administration.
[0164] In preferred embodiments of the sixth aspect, the
immunization scheme includes that the animal (e.g. the human)
receives a priming administration and one or more booster
administrations.
[0165] Preferred embodiments of the 6.sup.th aspect of the
invention comprise that the administration is for the purpose of
inducing protective immunity against C. jejuni. In this embodiment
it is particularly preferred that the protective immunity is
effective in reducing the risk of attracting infection with C.
jejuni or is effective in treating or ameliorating infection with
C. jejuni.
[0166] As mentioned herein, the preferred vaccines of the invention
induce humoral immunity, so it is preferred that the administration
is for the purpose of inducing antibodies specific for C. jejuni
and wherein said antibodies or B-lymphocytes producing said
antibodies are subsequently recovered from the animal.
[0167] But, as also mentioned the method of the 6.sup.th aspect may
also be useful in antibody production, so in other embodiments the
administration is for the purpose of inducing antibodies specific
for C. jejuni and wherein B-lymphocytes producing said antibodies
are subsequently recovered from the animal and used for preparation
of monoclonal antibodies.
[0168] Pharmaceutical compositions can as mentioned above comprise
polypeptides, antibodies, or nucleic acids of the invention. The
pharmaceutical compositions will comprise a therapeutically
effective amount thereof.
[0169] The term "therapeutically effective amount" or
"prophylactically effective amount" as used herein refers to an
amount of a therapeutic agent to treat, ameliorate, or prevent a
desired disease or condition, or to exhibit a detectable
therapeutic or preventative effect. The effect can be detected by,
for example, chemical markers or antigen levels. Therapeutic
effects also include reduction in physical symptoms, such as
decreased body temperature. The precise effective amount for a
subject will depend upon the subject's size and health, the nature
and extent of the condition, and the therapeutics or combination of
therapeutics selected for administration. Thus, it is not useful to
specify an exact effective amount in advance. Reference is however
made to the ranges for dosages of immunologically effective amounts
of polypeptides, cf. above.
[0170] However, the effective amount for a given situation can be
determined by routine experimentation and is within the judgement
of the clinician.
[0171] For purposes of the present invention, an effective dose
will be from about 0.01 mg/kg to 50 mg/kg or 0.05 mg/kg to about 10
mg/kg of the DNA constructs in the individual to which it is
administered.
[0172] A pharmaceutical composition can also contain a
pharmaceutically acceptable carrier. The term "pharmaceutically
acceptable carrier" refers to a carrier for administration of a
therapeutic agent, such as antibodies or a polypeptide, genes, and
other therapeutic agents. The term refers to any pharmaceutical
carrier that does not itself induce the production of antibodies
harmful to the individual receiving the composition, and which may
be administered without undue toxicity. Suitable carriers may be
large, slowly metabolized macromolecules such as proteins,
polysaccharides, polylactic acids, polyglycolic acids, polymeric
amino acids, amino acid copolymers, and inactive virus particles.
Such carriers are well known to those of ordinary skill in the
art.
[0173] Pharmaceutically acceptable salts can be used therein, for
example, mineral acid salts such as hydrochlorides, hydrobromides,
phosphates, sulfates, and the like; and the salts of organic acids
such as acetates, propionates, malonates, benzoates, and the like.
A thorough discussion of pharmaceutically acceptable excipients is
available in Remington's Pharmaceutical Sciences (Mack Pub. Co., N.
J. 1991).
[0174] Pharmaceutically acceptable carriers in therapeutic
compositions may contain liquids such as water, saline, glycerol
and ethanol. Additionally, auxiliary substances, such as wetting or
emulsifying agents, pH buffering substances, and the like, may be
present in such vehicles. Typically, the therapeutic compositions
are prepared as injectables, either as liquid solutions or
suspensions; solid forms suitable for solution in, or suspension
in, liquid vehicles prior to injection may also be prepared.
Liposomes are included within the definition of a pharmaceutically
acceptable carrier.
[0175] As is apparent from the claim, the invention also relates to
related embodiments to the treatment and prophylaxis disclosed
herein: the invention also includes embodiments where
[0176] the polypeptide of the invention is for use as a
pharmaceutical, in particular for use as a pharmaceutical in the
treatment, prophylaxis or amelioration of infection with C.
jejuni;
[0177] the nucleic acid fragment of the invention or the vector of
the invention is for use as a pharmaceutical, in particular for use
as a pharmaceutical in the treatment, prophylaxis or amelioration
of infection with C. jejuni;
[0178] the transformed cell of the invention is for use as a
pharmaceutical, in particular for use as a pharmaceutical in the
treatment, prophylaxis or amelioration of infection with C.
jejuni.
[0179] the antibody, antibody fragment or antibody analogue of the
invention is for use as a pharmaceutical, in particular for use as
a pharmaceutical in the treatment, prophylaxis or amelioration of
infection with C. jejuni.
EXAMPLES
Material and Methods
Bacterial Strains and Plasmid
[0180] The bacterial strains used in this study included E. coli
SURE (Stratagene) and E. coli BL21 (DE3) (Stratagene) and the
plasmid was pTLJ03. Strains and plasmid originates from a NCTC
11168 C. jejuni ORF library (Parrish et al., 2004) available from
Geneservice. The expression clone set comprises >1,600 C. jejuni
ORF's and the expression vector pTLJ03 generates N-terminal
GST-His-tagged fusion proteins.
[0181] Strains were grown in LB media or the expression media
MagicMedia (Invitrogen) at 37.degree. C. pTLJ03 containing strains
were grown in media containing 50 .mu.g/mL ampicillin unless
otherwise specified. C. jejuni 11168H is a stable motile variant of
the reference strain C. jejuni NCTC 11168; its preparation is
described infra in the section headed "recombinant DNA techniques".
C. jejuni strains (NCTC 11168 and 11168H) were grown at 37.degree.
C. microaerophilic on blood plates (BaseII and 5% blood) in BHI
broth or biphasic (blood plates and BHI broth) with antibiotic when
needed (30 .mu.g/mL kanamycin or/and 50 .mu.g/mL streptomycin).
Expression Library
[0182] The library was originally created in E. coli SURE for
optimal storage. This strain does not contain the T7 polymerase and
for that reason the library was transformed to the E. coli BL21
(DE3) expression strain. The clones were grown separately overnight
in microtiter plates in 200 .mu.l LB media containing ampicillin
and subsequently the plasmids were purified as a pool and
transformed to the chemocompetent E. coli BL21 (DE3) strain. This
revealed an expression library consisting of 2304 clones (24
microtiter plates).
Immunoblot Assay
[0183] Individual clones were grown 16-20 hrs in microtiter plates
in MagicMedia for optimal expression. 2 .mu.l of the culture was
spotted on nitrocellulose membranes. The membranes were blocked in
blocking buffer 30 min., washed in PBS Tween and then incubated in
primary antibody (1:1000) at 4.degree. C. for 16-20 hrs. The
membranes were then washed in PBS Tween and incubated in secondary
antibody (Polyclonal goat anti-rabbit immunoglobulins/HRP, Dako)
for 1 hr. The reaction was visualised by chemoluminescence
(chemoluminescent substrate, Invitrogen). The primary antibody was
raised in rabbit immunised with a boiled-treated (100.degree. C.
for 1 h) C. jejuni Penner serotype 2 originally isolated from a
human patient. Rabbit serum from immunisations with the Penner
serotype 2 was chosen since it corresponds to the serotype used for
creating the commercial library (NCTC 11168). The serum was
preincubated with E. coli BL21 (DE3) before use to minimise
background reaction. To verify that the antigens also reacted
against human serum, a dot blot with 10 selected clones expressing
antigens and serum isolated from a patient infected with C. jejuni
Penner serotype 2 (Strid M A et al. 2001, "Antibody responses to
Campylobacter infections determined by an enzyme-linked
immunosorbent assay: 2-year follow-up study of 210 patients", Clin.
Diag. Lab. Immunol. 183:2553-9.) was carried out as described
above.
Clone Sequencing
[0184] Plasmid DNA was isolated from 100 ml E. coli BL21 (DE3)
cultures using MidiPrep (Qiagen). Sequencing was conducted by
Macrogen Inc. and the primer 5' GCT ATC CCA CAA ATT GAT AA 3' (SEQ
ID NO: 91).
Recombinant DNA Techniques
[0185] C. jejuni 11168H knock-out mutants were kindly provided by
Brendan Wren from the London School of Hygiene and Tropical
Medicine, University of London. Mutants were constructed via
insertion of the Km cassette into unique sites present in
pUC18-based recombinant plasmids containing random 1-kb fragments
from the C. jejuni NCTC 11168 genome library (Garenaux A et al.
2008, "Role of the Cj1371 periplasmic protein and the Cj0355c
two-component regulator in the Campylobacter jejuni NCTC 11168
response to oxidative stress caused by paraquat", Res Microbiol.
159: 718-26 and Parkhill J et al. 2000, "The genome sequence of the
food-borne pathogen Campylobacter jejuni reveals hypervariable
sequences", Nature 10; 403(6770):665-8). The 11168H knock out
mutant provided for this study is: Cj0034c. Subsequently the gene
knock-outs were transferred from the C. jejuni 11168H strain to the
C. jejuni 11168 strain to restore motility and spiral morfology.
Natural transformation was performed as described previously (Wang
Y and Taylor D E 1990, "Natural transformation in Campylobacter
species", J Bacteriol. 172: 949-55) with some modifications. C.
jejuni cultures grown overnight on BHI agar plates were collected
and resuspended in 12 ml BHI broth to OD.sub.600 of 0.001.
Bacterial suspensions in three dilutions were transferred to
sterilized Petri dishes, incubated at 37.degree. C. with no shaking
under micro aerobic conditions over night. 200 .mu.g cultures with
OD.sub.600 0.2-0.3 were transferred to sterilized tube with 1 ml
BHI and incubated at 37.degree. C. with shaking under micro aerobic
conditions 2 h. Then 10 ng of genomic DNA, purified with Qiagen
blood and tissue kit, of the mutants, was added each tube. After
additional incubation for 3 h, bacterial cultures were serially
diluted and plated on BaseII agar plates with antibiotics (50 mg/l
kanamycin). The agar plates were incubated at 37.degree. C. under
microaerobic conditions 3 days. The mutants were checked for curved
shape and motility before tested in assays.
INT407 Adhesion Assay
[0186] INT407 cells (representing intestinal cell line) were grown
in MEM (+glutamax) media (Invitrogen) added 25 .mu.g/ml gentamycin
and 10% heat inactivated fetal bovine serum in 5% CO.sub.2. Cells
were seeded at 2.5.times.10.sup.5pr well in 24 well plates,
incubated overnight and checked for 100% confluent monolayer. The
E. coli clones were grown overnight in MagicMedia broth at
37.degree. C. and C. jejuni on blood agar plates microaerophilic at
37.degree. C. Immediately before assay, the OD.sub.600 of the
bacteria was adjusted to 1 in PBS and 1 ml bacteria culture was
added the INT407 cells and cells were incubated with bacteria for 2
hours at 37.degree. C., then resuspended and diluted in PBS and
spotted on agar plates with appropriate antibiotics.
Electron Microscopy
[0187] To investigate, whether the C. jejuni mutant strain differed
morphologically from the wild type strain, a transmission electron
microscopy analysis was conducted. Initially, the bacterial
cultures were fixated in 1% glutaraldehyde (EMS, Hatfield, USA) for
30 minutes. To improve the adhesion of the bacteria, formvar coated
400-mesh copper grids were treated for 5 minutes with alcian blue
(Sigma-Aldrich). The alcian blue treated grids were placed on top
of cultures of C. jejuni NCTC11168 and C. jejuni NCTC11168L0034
(Cj0034c), respectively, and after 5 minutes of incubation, most of
the suspensions were removed from the grids with filter paper and
the grids were stained for 30 seconds with phosphotungstic acid
(BDH Chemicals). The grids were allowed to air-dry, and then they
were viewed in a Morgagni 268D transmission electron microscope,
and pictures were taken using a Mega-view III digital camera.
Motility Assay
[0188] A motility assay was carried out to ensure no altered
motility for the 11168L0034 mutant. 0.25% soft agar plates were
supplied with 1 .mu.l bacterial culture (OD.sub.600 adjusted to
0.1) in the middle of the plate and diameter was measured over a
time period.
Serum Resistance Assay
[0189] Serum sensitivity assays were performed by modification of
the method of Blaser at al (Blaser M J et al. 1985, "Susceptibility
of Campylobacter isolates to the bactericidal activity of human
serum", J Infect Dis. 151: 227-235.) C. jejuni strains were grown
overnight in Brucella biphasic cultures at 37.degree. C., washed in
PBS, pH 7.4, and adjusted to a concentration of 10.sup.3 CFU/ml. C.
jejuni cells (10-.mu.l aliquots) were incubated in 240-.mu.l pools
of whole human blood (venous blood), human serum (whole blood
incubated at 25.degree. C. 30 min, centrifuged 1000.times.g 10 min
at 4.degree. C. and supernatant isolated) and heat inactivated
human serum (56.degree. C. for 30 min) respectively for 30, 60, 90
and 120 min. Following the incubation period, CFU was enumerated on
BHI agar.
Biofilm and Autoagglutination
[0190] Cell-to-cell autoagglutination was assayed in PBS as
described by Misawa and Blaser (Misawa N and Blaser M J 2000,
"Detection and characterization of autoagglutination activity by
Campylobacter jejuni", Infect. Immun. 68: 6168-6175.)
[0191] Biofilm assay was made in 50 ml centrifuge tubes containing
25 ml inoculated Brucella broth with NCTC 11168 and the knock out
mutants in Cj0034c. A glass slide was added each tube and incubated
micro aerobic for 48 h. Then the slides were stained with crystal
violet and biofilm formation visualised.
Predictions of Protein Localization
[0192] Prediction of protein localization and amount of
transmembrane helixes was made by TMHMM 2.0 server (Moller S et al.
2001, "Evaluation of methods for the prediction of membrane
spanning regions", Bioinformatics, 17: 646-653).
[0193] The SignalP 3.0 server predicts the presence and location of
signal peptide cleavage sites in amino acid sequences. The method
incorporates a prediction of cleavage sites and a signal
peptide/non-signal peptide prediction based on a combination of
several artificial neural networks and hidden Markov models. The
LipoP 1.0 server produces predictions of lipoproteins and
discriminates between lipoprotein signal peptides, other signal
peptides and N-terminal membrane helices in Gram-negative bacteria
(Juncker A S et al. 2003, "Prediction of lipoprotein signal
peptides in Gram-negative bacteria", Protein Sci. 12:1652-62).
Protein Purification
[0194] His-tag purification was made with the already
GST-His-tagged constructed vector from Geneservice. An overnight
pre-culture of E. coli BL21(DE3) containing the vectors was 50-fold
diluted to inoculate 1000 ml LB medium containing appropriate
antibiotics. The cultures were incubated with shaking at 37.degree.
C. to an OD.sub.600 of 0.5, then induced with 10 mM IPTG and
incubated with shaking for 16 hours at 30.degree. C. After
induction, cells were lysed on ice in 20 ml lysis buffer (50 mM
NaH.sub.2PO.sub.4, 300 mM NaCl, 10 mM imidazole 10% glycerol) by
addition of 1 mg/ml lysozyme followed by sonication. Lysates were
cleared by centrifugation at 15,000.times.g for 30 min. Proteins
were purified by nickel affinity chromatography using the Ni-NTA
resin (Qiagen) equilibrated with lysis buffer and eluted with 250
mM imidazole. Eluted proteins were concentrated and dialyzed
against 25 mM HEPES pH 7.5, 50 mMNaCl, 10% glycerol.
Mouse Vaccination and Challenge Studies
[0195] To study ability of the proteins to protection against
infection, immunization of mice were carried out. Cj0404 (SEQ ID
NO: 13) was tested together with other putative vaccine candidates
for its ability to protect against C. jejuni infection. Mice (10 in
each group) were immunized with 5 .mu.g/dose, except one with 1.6
.mu.g/dose (Cj1371c), along with adjuvant (GNE, Intervet, NL). Four
weeks later, the mice (Balb/c for colonization and CH3/HeN for
invasion) were treated for three days with streptomycin (5 g/l in
drinking water) and challenged orally one day later with C. jejuni
81116 (6.times.10.sup.5 CFU, colonization study) and 72Dz/92
(5.times.10.sup.7 CFU, invasion study) respectively. Balb/c mice
6-8 weeks (female) were used in groups of three. One fresh faecal
dropping was collected and weighted from each animal and dilutions
were made in order to determine CFU/gram faeces. Faecal samples
were collected from the Balb/c mice regularly in 23 days. Necropsy
was prepared one week after challenge of the CH3/HeN mice, spleen
and liver were collected and CFU/organ were detected.
Results
[0196] Identification of C. jejuni Antigens
[0197] With the aim of identifying immuno-reactive C. jejuni
proteins plasmid DNA was isolated from a pooled mixture of
commercial library clones established by Parrish et al (Parrish J R
et al. 2004, "High-throughput cloning of Campylobacter jejuni ORfs
by in vivo recombination in Escherichia coli", J Proteome Res. 3:
582-6.) with C. jejuni NCTC11168 ORFs in the plasmid pTLJO3
(Parrish J R et al. 2004). Plasmid DNA was transformed into E. coli
BL21 to allow expression from the T7 promoter. The resulting
transformants were individually spotted on a nitrocellulose
membrane and reacted with serum isolated from a rabbit infected
with a C. jejuni human clinical isolate (serotype 2). The screening
revealed several immunogenic E. coli clones that selectively
reacted with the serum. Inserts in plasmids isolated from the
transformants that repeatedly proved as most immunogenic were
selected for sequencing and from a total of 2304 clones, 52 inserts
were sequenced representing 25 genes encoding potential antigens.
The identified C. jejuni genes were classified according to their
predicted function.
[0198] To confirm that the identified antigens also are functional
in humans we reacted 10 of the 25 clones (Cj0034, Cj0203, Cj0404
(SEQ ID NO: 13), Cj0525c, Cj0645, Cj0917c, Cj1094c, Cj1371,
Cj1382c, Cj1632c) with human antiserum obtained from a patient
infected with a C. jejuni Penner serotype 2 and found in all cases
a positive reaction. This result supports that the antigens
reacting with the mouse antiserum also are antigens in humans.
Prediction of Protein Localization
[0199] Prediction of localization of the proteins and amount of
transmembrane helixes was made: 14 out of 25 proteins are predicted
to contain one or more membrane helixes, two of them further with a
signal peptide. Other ten of the proteins are predicted to be
located externally, where three of them harbor a signal peptide.
None of the proteins were predicted to contain a lipoprotein signal
peptidase.
Several Antigens Support Host Cell Adhesion
[0200] Adhesion of C. jejuni to host cells forms the first
important step in the infection process. With the aim of addressing
whether the identified antigens contribute to host cell invasion a
selection of 10 E. coli clones expressing C. jejuni antigens were
investigated for their ability to adhere to the intestinal
epithelial cell line, INT407. Interestingly, expression of three of
the C. jejuni antigens enhanced the ability of E. coli BL21 to
adhere to INT407 cells, Cj0034c, Cj0404 (SEQ ID NO: 13) and Cj1371.
Subsequently, gene-specific C. jejuni mutations were constructed in
the corresponding genes, and the resulting mutants were examined in
the same cell adhesion assay. While the absence of Cj0404 and
Cj1371 did not affect the ability of C. jejuni to adhere to INT407
cells, inactivation of Cj0034 dramatically reduced adhesion
suggesting that Cj0034 may contribute to establishment of C. jejuni
in host organisms.
[0201] Further characterization of the Cj0034 mutant C. jejuni
strain revealed, that the mutation does not result in major
structural changes of the bacterial cell morphology as visualized
by electron microscopy. Also, the inactivation of Cj0034 did not
influence serum resistance, motility, autoagglutination and biofilm
formation, when compared to wild type strain.
Antigens as Vaccine Candidates
[0202] Five identified antigens were selected to test as vaccine
candidates in two Campylobacter oral challenge mouse models; one in
C3H/HeN mice in which invasion in liver and spleen was measured and
the other in Balb/c mice in which shedding faecal was determined.
The challenge study showed a reduced invasion into spleen and liver
for at least two of the proteins; Cj0525c and Cj0404 (SEQ ID NO:
13). No decreased colonization for any of the proteins was
observed.
Further Examples
C. jejuni Antigen Expression
[0203] 30 genes encoding potentially antigenic C. jejuni proteins
were identified and checked in NCBI and presence in the clone
library. Of the 30 suggested gene sequences, 19 were commercially
available and purchased from Life Sciences. All 19 were used for
cloning in expression plasmid (with his-tag) and transformation
into the expression strain E. coli BL21. Two of the constructs were
not transferred to E. coli BL21 and after several attempts we did
not proceed, which left 17 successful transformants.
[0204] We also separately PCR-cloned from Campylobacter jejuni
11168. Thus Cj0404, Cj0788, Cj0892 and Cj0424 were cloned into
plasmid with his-tag and transformed into E. coli BL21. Previous
work suggested that Cj0404 was antigenic (Clin Vaccine Immunol.
2012 February; 19(2):113-9) whereas Cj0424 was identified by
us.
[0205] Preliminary tests were performed with the 4 C. jejuni genes
transformed in BL21.
[0206] Further, several inductions were made with the 17 C. jejuni
genes transformed in E. coli BL21. Media and induction protocols
were tested. "Magic media" was seen to induce relevant sized
proteins as can be seen from the SDS gels shown in FIG. 1. It was
demonstrated that 7 C. jejuni clones were induced (circle
markings). Overnight cultures were run on the 12% SDS gel. All
experiments were performed at least twice with essentially the same
results.
Protein Purification and Verification of Immunogenicity
[0207] The C. jejuni transformed clones were tested for antibody
recognition in a dot blot assay.
[0208] The four selected clones produced from PCR cloning were
tested for expression and antibody antigenicity in a dot blot assay
against human sera (dot blot 1 shown in FIG. 2).
[0209] The 7 E. coli clones that could be induced to produce C.
jejuni proteins were tested for reaction with antisera from rabbits
immunised with five different Campylobacter strains of five
different isolates. All clones reacted to all sera.
[0210] All experiments were performed at least twice with
essentially the same results.
[0211] Since all antigens were recognised by rabbit antisera, they
were subsequently tested with sera from patients that were shown to
have antibodies against Campylobacter jejuni tested in the
diagnostic test that runs at the Danish State Serum Institute
(Strid et al. Clin Diagn Lab Immunol. 2001 March; 8(2):314-9).
[0212] The sera were randomly picked but all exhibited antibodies
against Campylobacter jejuni. Patients with no antibodies to C.
jejuni by ELISA are not expected to react in the dot blot assay
(data not shown). Examples of the dot blots are seen in FIG. 2.
[0213] From the dot blots it is seen that all patients found to
have antibodies against C. jejuni by a diagnostic ELISA react also
react with colonies of E. coli BL21 that express C. jejuni
antigens. In many cases the negative control being an E. coli BL21
without a plasmid is seen to also react with the sera This is
expected since humans have encountered E. coli infections and are
colonised in the gut.
IN CONCLUSION
[0214] We have identified 30 C. jejuni genes that are be
potentially antigenic and recognised by antibodies against
Campylobacter. We have been able to show that 7 of these are indeed
able to be recognised by antibodies raised in humans after a
natural infection with Campylobacter jejuni (diarrea).
Sequences of Proteins of the Invention:
[0215] The protein sequences of the invention mentioned in the
above examples are related to the sequences in the sequence listing
as follows:
TABLE-US-00001 Aa sequence Designation SEQ ID NO: 1: Cj0251c SEQ ID
NO: 2: Cj1464 SEQ ID NO: 3: Cj1406c SEQ ID NO: 4: Cj0579c SEQ ID
NO: 5: Cj0158c SEQ ID NO: 6: Cj0592c SEQ ID NO: 7: Cj0783 SEQ ID
NO: 8: Cj0371 SEQ ID NO: 9: Cj0424 SEQ ID NO: 10: Cj0944c SEQ ID
NO: 11: Cj0111 SEQ ID NO: 12: Cj0596 SEQ ID NO: 13: Cj0404 SEQ ID
NO: 14: Cj0606 SEQ ID NO: 15: Cj1178c SEQ ID NO: 16: Cj1357c SEQ ID
NO: 17: Cj0144 SEQ ID NO: 18: Cj0262c SEQ ID NO: 19: Cj0887c SEQ ID
NO: 20: Cj1729c SEQ ID NO: 21: Cj0136 SEQ ID NO: 22: Cj0886c SEQ ID
NO: 23: Cj1365c SEQ ID NO: 24: Cj0279 SEQ ID NO: 25: Cj1677 SEQ ID
NO: 26: Cj0628 SEQ ID NO: 27: Cj1476c SEQ ID NO: 28: Cj0478 SEQ ID
NO: 29: Cj0007 SEQ ID NO: 30: Cj0479
[0216] For easy reference, the protein sequences of the invention
are set forth in one-letter amino acid code in the following:
TABLE-US-00002 SEQ ID NO: 1 MAYEDEEDLN YDDYENEDEE YPQNHHKNYN
YDDDDYEYDD DNNDDDFYEM D 51 SEQ ID NO: 2 MINPIQQSYV ANTALNTNRI
DKETKTNDTQ KTENDKASKI AEQIKNGTYK IDTKATAAAI 60 ADSLI 65 SEQ ID NO:
3 MKKILILLTL CAFAFGASEC DRKIDRINKE ISFSKAHNDT ARTLSLELAL KQVQNDCAKD
60 PMFYDKKLEA KKLKEQEVEK IEKELDALKE QKDYMSKAEY KAKKEALKEQ KEKIKK
116 SEQ ID NO: 4 MSFGEIIVIL VVAILVLGPD KLPEAIVQIA KILKAVKRNI
DDAKSSIEKE IRINDLKEEA 60 KKYKDEFSST NENIRKKLSF EEFDDLKRDI
LDKTKVDLTF DSRDDKVKNN LSGQNLNTEE 120 KPNLSKLETQ DKNGKINV 138 SEQ ID
NO: 5 MQKAKILIAL SFFLLVLSAC SNDEKNISKT QNTDQEVVQI EQNDEKTELS
DSNLPLPVDD 60 EAQSSNDEHE VNPSIINSLY KQKCATCHGE KGELKPKNST
AIKTLSNKIF IQKIKTIKDK 120 NHSFLSDEQI QNLADFINKG K 141 SEQ ID NO: 6
MRRLSILLAI LIVINITACD SKTENYYKNL PSEAKEKAKE CKESGTLSED CINALKVGVK
60 PTNEEGKYSP NTPKKSDNQI LEALKQNDLK KEKTTKDINQ SSENNESIII
PPIAETPSEI 120 YPSKTTENNQ SSIFSDDVNM TQEK 144 SEQ ID NO: 7
MMKKKLVLLG SAAVVFFAAC AMNSGVSSEQ IGLRKASLEN ENKVNLVEAN FTTLQPGEST
60 RFERSYENAP PLIPHAIEDL LPITKDNNMC LSCHDKAIAA DAGATPLPAS
HYYDFRHNKT 120 TGDMISDSRF NCTQCHVPQS DAKPLVGNSF KPEFKNEQLK
SRSNLIDVIN EGVK 174 SEQ ID NO: 8 MKKIKKIIQI GMIGGLAAVA GGALAGCGSN
NDNADTLNQA ANAQGAFVII EETAPGQYKI 60 KDQYPSDETR VVLKDLNGTE
RILSKEEMDA LIKEEAAKID NGTSNLTKDN GQISSGGLSL 120 GETLLASAAG
AILGSWIGSK LFNNQNFANQ QRGAFSNQSA YQRSVNSFNK AGTTSSASSA 180
KKSGFFGGGS KATSSSSSFG S 201 SEQ ID NO: 9 MTKFLSICSL IAMLLSGCGS
DFPGQPSDVA RVQQNKYPNG NLKKEIPYNK DSRIHGLKRA 60 FYDNGQLRAE
ENYKNGKKDG ISREYSRNGQ LLEEVHFKDN RGYGDFASYY ENGNMRAKGK 120
LLGYNEDGMP EFEGNYKEYY ENGTLMCDYN FDNKGKFDGV QKRYDENGAL EDEENYKNGL
180 KNGVFREYKK GEIVREEEYK NGILVAKPKN 210 SEQ ID NO: 10 MKKIFLSVFL
VLSLNAQNLE IDKIRTDLYS KSGANVLKKV EISLEFDGNN LKENENKLID 60
AVNTVISGFF YEDIFTEIGK NNFKKTLEKF LDKKYKIKLD DIYIISLSGV EKFDLEEFKR
120 FLESTEAKEK GMGSEVKKAL ENLEVPKTQV PSVEKIPTPS VPNLEVKQVE
QLFKDPDEEN 180 KNDNGEINID NLNTPKMTPD IEEKIKRDLI ANPPQIFKEN
NASKPYHLPQ TGYDIKLDEN 240 STQN 244 SEQ ID NO: 11 MKNYGLSNLN
SFLLALAIYI SIVILVFFRL VSEVEPAIQY TDIKDSFVDI ELAEPSKQVI 60
TQSNTPKEIQ KPTEQIDIEK LFAQTTNKTV KTEDIDQKAS NFNELFGNIK EIQEEKTTKI
120 QSSAKSGISS APKPQASELV KQLNDSLLQE ESSTQGESTK AQKIGIYDEF
LGKVVRIITQ 180 RWTQYYPNSE KISVKVKIFI DENGKFGYTS VEKSGNPLYD
AKVAEFLESQ KGKFITYPPQ 240 NKNISITMNL RDEVKVKND 259 SEQ ID NO: 12
MKKFSLVAAT LIAGVVLNVN AATVATVNGK SISDTEVSEF FAPMLRGQDF KTLPDNQKKA
60 LIQQYIMQDL ILQDAKKQNL EKDPLYTKEL DRAKDAILVN VYQEKILNTI
KIDAAKVKAF 120 YDQNKDKYVK PARVQAKHIL VATEKEAKDI INELKGLKGK
ELDAKFSELA KEKSIDPGSK 180 NQGGELGWFD QSTMVKPFTD AAFALKNGTI
TTTPVKTNFG YHVILKENSQ AKGQIKFDEV 240 KQGIENGLKF EEFKKVINQK
GQDLLNSAKV EYK 273 SEQ ID NO: 13 MENQKNEFDD IILEKSNKSE KVKKILLRVI
ALVILFLAIM IVMKLINGSG DENTQNQSVL 60 PSEPIATQDN NNDTSFESMP
ITDNTSAEDQ FEALRKQFQD EQNTTQNTTT SSSNNNDTTN 120 FAMPDQEVPA
EPTATTSANT TPQASTPKQE VTQTAKSKEE AKKQTAVKKE KESAKQTPKK 180
EQNANDLFKN VDAKPVHPSG LASGIYVQIF SVSNLDQKSK ELASVKQKGY DYKLYKTTVG
240 SKEITKVLIG PFEKADIAAE LAKIRKDIAK DAFSFTLK 278 SEQ ID NO: 14
MKKKIVLIIL IAILGSVGAY FIFFNNDEKI SYLTQKIQKK DISQTIEAVG KVYAKDQVDV
60 GAQVSGQIIK LYVDVGTHVK QGDLIAQIDK DKQQNDLDIT KAQLESAKAN
LESKKVALEI 120 ANKQYQREQK LYAAKASSLE NLETQKNNYY TLKANVAELN
AQVVQLEITL KNAQKDLGYT 180 TITAPMDGVV INVAVDEGQT VNANQNTPTI
VRIANLDEME VRMEIAEADV SKIKVGTELD 240 FSLLNDPQKT YHAKIASIDP
ADTEVSDSST SSSSSSSSSS SSSSSNAIYY YAKFYVANKD 300 DFLRIGMSIQ
NEIVVASAKA VLAVPTYAIK SDPKGYYVEI LENQKAVKKY VKLGIKDSIN 360
TQILEGVNED EELIVSSSAD GLAPKMKLRF 390 SEQ ID NO: 15 MKILLLNENP
VVSRLVSLSA KKMSYDFEEL NAYSENLGNY DVIVVDSDTP APLKILKEKC 60
DRLIFLAPRN QNVEDIDAQI LQKPFLPTDF LNLLNNKDAN KHTSIDLPML SNDENPYADI
120 SLDLDNLNLD DLPDENSLDI NSEGMEDLSF DDDAQDDNAN KTLETQNLEH
ETIKEQTQED 180 TQIDLDLTLE DGESEKEDLS QEHTALDTEP SLDELDDKND
EDLEIKEDDK NEEIEKQELL 240 DDSKTNTLEM QEELSESQDD NSNKTLETQN
LEHDNLEQET IKEQTQEDTQ IDLDLTLEDG 300 ESEKEDLSQE HTALDTEPSL
DELDDKNDED LEDNKELQAN ISDFDDLPEV EEQEKEMDFD 360 DLPEDAEFLG
QAKYNEESEE NLEEFAPVVE EDIQDEIDDF ASNLSTQDQI KEELAQLDEL 420
DYGIDSDNSS KVLEDFKDEP ILDDKELGTN EEEVVVPNLN ISDFDTLKES DIQEALGEEI
480 LEKNEEPIVS DVTKDDNSEE IVNELSQSIA GAITSSIKDD TLKAALKGMN
MNININISFK 540 ED 542 SEQ ID NO: 16 MKKNILRLGI VVLVLLIAGV
LWLNNDINQK KEDEANKNAI AANADFSLLS DDDPNFEKWG 60 KVFPEQLKMY
LTVEKEEPKA TEFGGNLAYS KLIRFPQLTI LWAGYPFSLD FNEERGHFWV 120
QVDQMKTARN NKDFLNAHGL AAFKGQPAAC MNCHSGWTPW LIKNVAKGDF TAFNSTNYWT
180 MIKNIPAVDG IVENSPEHAG PHGGKRMGVT CADCHNPNDM SLRLTRPAAI
NALVSRGYEK 240 DPVQGVKATR EEMRTLVCSQ CHVEYYFKPT GEKVKVMGET
IVDDSSKKWW NGTQKNYDEY 300 EFWRDGNKVK EIETDGIVLT FPWSEWKKGQ
PFRIEMLDDY YDKVRGVFGA DFTHKLTGAQ 360 IIKIQHPESE LYSGGVHAAN
GVSCVDCHMP YVREGAKKVT QHNITSPLRD INSACKSCHK 420 QSEDYLKAQV
LDIQNSVAHD QRTAEYAIVS LIMDTKKLRD ELGNMEKFQS DGKADAKKIS 480
EELKEVLELH RKAQMRADFV NAENSTGFHN PREASRMLLQ AVDMARMGQT KLVEIAAANG
540 IKDFKTSNLG FEDIQKFNPG ELYYKVDVNN HKAGERYYAD EKDVNGNPPK
ELLEHDKELA 600 PYNYQVIDKK 610 SEQ ID NO: 17 MKSVKLKVSL IANLIAVVCL
IILGVVTFIF VKQAIFHEVV NAEINYVKTA KNSIESFKAR 60 NSLALESLAK
SILKHPIEQL DSQDALMHYV GKDLKNFRDA GRFLAVYIAQ PNGELVVSDP 120
DSDAKNLDFG TYGKADNYDA RTREYYIEAV KTNKLYITPS YIDVTTNLPC FTYSIPLYKD
180 GKFIGVLAVD ILAADLQAEF ENLPGRTFVF DEENKVFVST DKALLQKGYD
ISAIANLAKT 240 KEDLEPFEYT RPKDGNERFA VCTKVSGIYT ACVGEPIEQI
EAPVYKIAFI QTAIVIFTSI 300 ISVILLYFIV SKYLSPLAAI QTGLTSFFDF
INYKTKNVST IEVKSNDEFG QISNAINENI 360 LATKRGLEQD NQAVKESVQT
VSVVEGGNLT ARITANPRNP QLIELKNVLN KLLDVLQARV 420 GSDMNAIHKI
FEEYKSLDFR NKLENASGSV ELTTNALGDE IVKMLKQSSD FANALANESG 480
KLQTAVQSLT TSSNSQAQSL EETAAALEEI TSSMQNVSVK TSDVITQSEE IKNVTGIIGD
540 IADQINLLAL NAAIEAARAG EHGRGFAVVA DEVRKLAERT QKSLSEIEAN
TNLLVQSIND 600 MAESIKEQTA GITQINDSVA QIDQTTKDNV EIANESAIIS
STVSDIANNI LEDVKKKRF 659 SEQ ID NO: 18 MQSINSGKSV GISAKLTLWV
GILVVLILAI TSAISYFDSR NNTYELLKDT QLKTMQDVDA 60 FFKSYAMSKR
NGIQILANEL TNRPDMSDEE LINLIKVIKK VNDYDLVYVG FDNTGKNYQS 120
DDQILDLSKG YDTKNRPWYK AAKEAKKLIV TEPYKSAASG EVGLTYAAPF YDRNGNFRGV
180 VGGDYDLANF STNVLTVGKS DNTFTEVLDS EGTILFNDEV AKILTKTELS
INIANAIKAN 240 PALIDPRNQD TLFTAKDHQG VDYAIMCNSA FNPLFRICTI
TENKVYTEAV NSILMKQVIV 300 GIIAIIIALI LIRFLISRSL SPLAAIQTGL
TSFFDFINYK TKNVSTIEVK SNDEFGQISN 360 AINENILATK RGLEQDNQAV
KESVQTVSVV EGGNLTARIT ANPRNPQLIE LKNVLNKLLD 420 VLQARVGSDM
NAIHKIFEEY KSLDFRNKLE NASGSVELTT NALGDEIVKM LKQSSDFANA 480
LANESGKLQT AVQSLTTSSN SQAQSLEETA AALEEITSSM QNVSVKTSDV ITQSEEIKNV
540 TGIIGDIADQ INLLALNAAI EAARAGEHGR GFAVVADEVR KLAERTQKSL
SEIEANTNLL 600 VQSINDMAES IKEQTAGITQ INDSVAQIDQ TTKDNVEIAN
ESAIISSTVS DIANNILEDV 660 KKKRF 665 SEQ ID NO: 19 MRITNKLNFT
NSVNNSMGGQ SALYQISQQL ASGLKIQNSY EDASTYIDNT RLEYEIKTLE 60
QVKESTSRAQ EMTQNSMKAL QDMVKLLEDF KVKVTQAASD SNSQTSREAI AKELERIKES
120 IVQLANTSVN GQYLFAGSQV ANKPFDSNGN YYGDKNNINV VTGAGTESPY
NIPGWDLFFK 180 ADGDYKKQIS TNVSFTDNRW DLNKDPDKTK YLTGDSKWQQ
LIGQSYVKDN SLDADKDFEY 240 DDSKLDFPPT TLYVQGTRPD GTSFKSAVLV
KPEDTLEDVM ENIGALYGNT PNNKVVEVSM 300 NDSGQIQITD LKQGNNKLDF
HAVAFTPQAD DKTELNNIIQ AAQDEGITME DVTNRVMTAA 360 LGNPNNGDIT
NLNNPVTIQI NGQNFEIDLK QTDFIKSKMT DTDGNAANGA DYDNVYFEKN 420
GNTVYGNVSQ VIKGSNAYAT DSTKLSEVMA GDSLNGTTLN LKVNSKGGNS YDVTINLQTS
480 TVSYPDPNNP GQTISFPIMH TNPATGNSGV VTGSNDITYG QINDIIGMFA
ADKIPTTTIQ 540 ANNGQINNAD YTQIQQLMKD SQATVDVSMD YKGRISVTDK
LSSGTNIEIS LSDSQSGQFP 600 APPFTTTSTV QNGPNFSFSA NNSLTIDEPN
VDIIKDLDSM IDAVLKGNMR ADSESENPRN 660 TGMQGALERL DHLADHVSKL
NTTMGAYHNT IEGVNTRTSF LSVNVQSIKS NVIDVDYGEA 720 MMNLMQVQLA
YQASLKASTT ISQLSLLNYM 750 SEQ ID NO: 20 MMRSLWSGVS GLQAHQVAMD
VEGNNISNVN TTGFKYSRAD FGTMFSQTVK IATAPTDGRG 60 GSNPLQIGLG
VSVSSTTRIH SQGSVQTTDK NTDVAINGDG FFMVSDDGGL TNYLTRSGDF 120
KLDAYGNFVN NAGFVVQGWN INWDDQTIDS SRTPQNIFID PGMHIPAAKS TEVAIKANLN
180 SGLNIGTSSR NLYALDSVHG WNTKTQRAED ENDTGTTQFY TTSKNSVEVT
EKGVDAGSLF 240 NAKGQGLNLR DGQGIWVSYA DATYSTNKVG VNAFDPNLQQ
NQTAAFWGTA NQKVNLDITL 300 NGVRIQNADI QSIDDAIAYI NTFTAPTDTR
DGTGVKAVKN KDGSGIDFVN DNADGTTDNM 360
KNINLVVANT NTAGELWNAV WNNNNQTFTF NNNGNGQAGT PTINKNGSSL WTATNITFTP
420 QPPQAATNVQ LTGGLNAQII TAHKYIYSSN PVDIGPMYNP DGGPAFQPGA
NATTRPTEPG 480 SAAYWDAVNG GLLNTNVRTF RTTEDLRELL QRDARYGVDY
DGSGTFAAAD INQNIKVVVT 540 ADGHFAISNA NEQSTVPPNA INGVGNATTT
DPKNMSFNIT AYSNKQGTVS TNDAFTAIFK 600 AFDGPLVIGN QIKESEQLKL
SAFSAGLEIY DSLGSKHTLE VQFVKQSTTQ DGGNEWQMII 660 RVPEPAEINT
TGEGPNNIIV GTARFNNDGS LASYTPRTIN FSPNNGAAPN QQIKLSFGTS 720
GSNDGLVSSN SASTLTGQAT DGYTSGNLKP DAIRVDDKGN ILGEFTNGKT FAVAKIAMAS
780 VANNSGLEEI GGNLFKVTAN SGNIVVGEAG TGGRGEMKTS ALEMSNVDLS
RSLTELIIIQ 840 RGYQANSKTI STSDQMLQTL IQLKQ 865 SEQ ID NO: 21
MAKIRIHEIA KELGYDSKEI IEKANELGLG IKTASNAVEP EIAAAIYEYI QTREIPEAFK
60 KNIKTPTAKK PKKENIKEQE KLNESEKKEP KKEEKLKQEV KKEELKIEKE
NAKEEEKQEI 120 IDAHKPQSLA SATLAKRRGL VIVKKKKDEE EIQVKKEEVK
NSNDISINNE ERLSLKTMFS 180 NADESLKKKK KEKKSFVASK KESTEKMNFL
DEHDFGDISL DDEDEVVLPD FSVKEQEKPQ 240 NINKKQPNFI RQAVGNSAGF
GFEGGIQRRS RKKPSKKIEK KEVEEVGSVA ISKEIRVYEF 300 ADKIGKSTSE
VISKLFMLGM MTTKNDFLDE DAIEILAAEF GIEINIINEA DEFDYVKDYE 360
EETDEKDLVT RAPVITIMGH VDHGKTSLLD YIRKSRVASG EAGGITQHVG AYMVEKNGRK
420 ITFIDTPGHE AFTAMRARGA SITDIVIIVV AADDGVKPQT KEAINHAKAA
GVPIIIAINK 480 MDKEAANPDM VKTQLAEMEI MPVEWGGSYE FVGVSAKTGM
GIEDLLEIVL LQADILELKA 540 NPKSFAKASI IESSVQKGRG AVATVIVQNG
TLTVGSTVVA GEAYGKVRAM SDDQGKALKE 600 IKPGECGVIV GLSEVADAGE
ILIAVKTDKE AREYANKRHE YNRQKELSKS TKVSIDELGA 660 KIKEGNLKAL
PVILKADVQG SLEALKASLE KLRNDEIKVN IIHSGVGGIT QSDIELASAS 720
ENSIVLGFNI RPTGEVKERA KDKGVEIKTY NVIYNLLDDV KALLGGMMSP IISEEQLGQA
780 EIRQVINVPK IGQIAGCMVT EGVINRGAKI RLIRDGVVVY EGNVSSLKRF
KDDAKEVAKG 840 YECGVGIEGC DDMRVGDYIE SYKEVEEQAS L 871 SEQ ID NO: 22
MLAPGMGEWV YKANLFLFGE FAYYYPFFLF ILNYVYYKRN YKLANFTRRE LFGIGFAFFS
60 SLLLFAVFYP NSGYILELAY AIFSTILGHT GSGIFALLLL LFSLVLLFPK
FAKEILKIEL 120 DFTYLLKVEQ AFKSLLMRVF GGENEKEDVG KSEPIVPKLN
ILQDSIYGNL QINKKGETNN 180 LEQIIKDSNI NASKNSITTA KENFEKLKNQ
ILDETIEIDK QSLKESRSFV HEHSQQVRNF 240 AQKASKMSIS LDEDFNFISE
EEVDMIPERF LKPKKLEDIK QIDTNKNLDE PSYKRKNIEI 300 PVSNQEVKPK
IFTKELELRE NLIKKEKLEQ EYKAYQNEIL ENKVKQEIKK LEEYDAINSS 360
DIIEGNKYSF NSPKTIKTET EESDKINENK NLDKADNIFE FAPIVEELNH PYIEPTPIKN
420 INEIVIEEKN TLDFIQNTET KIDNEKTNDQ EIKLQKAVLA KEIAINQALL
REIEQGEIEK 480 PKDFTLPPLD FLANPKEHKQ EINESEIDKK IYNLLEKLRR
FKIGGDVIST YVGPVVTTFE 540 FRPSADVKVS RILNLQDDLT MALMAKSIRI
QAPIPGKDVV GIEVPNDEIQ TIYLREILQS 600 EVFKNAKSPL TIALGKDIVG
NAFVTDLKKL PHLLIAGTTG SGKSVGINSM LLSLLYRNSP 660 KTLRLMMIDP
KMLEFSIYND IPHLLTPVIT DPKKAVNALS NMVAEMERRY RLMADAKTKN 720
IENYNEKMKE LGGEKLPFIV VIIDELADLM MTAGKDVEFY IGRLAQMARA SGIHLIVATQ
780 RPSVDVVTGL IKANLPSRIS YKVGQKIDSK VILDAMGAES LLGRGDCLFT
PPGTSSIVRL 840 HAPFASEFEI EKIVDFLKDQ QSVEYDESFL KDQQSVGVTT
NESFDGEADE LYEEAKRVIL 900 EDGKTSISYL QRRLKIGYNR SANIIEQLTQ
NGILSEPDAK GQREIL 946 SEQ ID NO: 23 MKKFFCLTLV CKLFALSEFE
LHHIDKVHKL GYSGDTIIIG VADDAFNQDH ISLKDKILKS 60 TYPTDTAGKQ
LIPDLKKSTH GSHVAGIAVG AKIGDSKPYG VAYGAKFYGA GVFPNGSYTQ 120
IPDIYNFFKD VSIINNSWGI NFYPYFNLKA SNSGLVDCTQ TNQGTSYNIC NTPLEYVMKA
180 DKVANDMMRL SKDKGVLNVF AAGNEGILSP ALHAILPSYD ESLRAWLAVG
ALDANEITLE 240 SDGTLIIKSQ GLADFSNGFK GATNFSLVAA GVNINNVDSS
TNDKFTKKSG TSMAAPMVSG 300 TAALVKQNFP FLDGKQIADI LLSTANKNYK
APKFTVKQVT DGTNQPKFLI VYISQDPPGI 360 EDEIKRDLKQ LYNGIQVQVN
GQWIDYSDYI WDNRDSAQSQ KLNTSTISSI NGVVRVEKEE 420 LFGQGILDAQ
KALKGLSILD ANRLSDQDVL KYEQEPNTAY YTINTAGYDA EFSNDISQRK 480
WDESTHLSSA INKPTHLANL NIGLSKEGEG ILIISGQNTY EGATLIKQGE LKLKGKVKNN
540 AYVEQKAILS GNGIVGQNLN NKGIVRPGNE DLNDLTVQGT YTQEGVDSKL
QLDFGNYKNS 600 KLIAKTYDIK SGNLEYIPLP KYYILNKPVK INLGDLEKSL
SSFNHVLIQN TYALNFDFVL 660 SDDLVSINKT LIKPNLKPNA YEIPNTSLGN
ALRQLRSRAD LSQTYQEFFA SLDNGIDVKT 720 KLNRIEGSGY LSTFSNHNQS
NLMQNNMLFT LHPLNINNFA QNNNILLAST YLPRIFSNEE 780 YFWHLTPSYK
YYKDKDFSGQ KTGANISLGE NFSSGFLAYA LSLSSAKFNF NNGSDLKSYN 840
MDLLLNYNHD LDFIKILSGL GIGVGFNTLN RFVVEQPIEG KYKTLQTSAQ LGVTKDIILG
900 QDFIFNPLMY FTHSFFYQED FKENKSPFAK NYESLKHHSI NANLGFNLAK
NIEQDDYQAS 960 FSTFVIFEKR IYGRTLENKA SFVDFPIAFI QKYKLKDNIL
SQGFNSEFLY KNNVFWQFML 1020 MNRFSHNAYE LHLMSSVGKR F 1041 SEQ ID NO:
24 MPKRTDIKSI LLIGSGPIVI GQACEFDYSG TQAAKTLKEL GYRVVLINSN
PATIMTDPEF 60 ADATYIEPIT KESILSIIKK EKIDAILPTM GGQVALNVAM
EVYESGLLGD VKFLGANPEA 120 IKKGEDRQVF KECMKKIGMD LPKSMYAYNY
DEALKAVDEI DFPLMIRASY TLGGAGSGVV 180 YNMDEFKELT NTALALSPIH
EILIEESLLG WKEYEMEVIR DRADNCIIVC SIENIDPMGV 240 HTGDSITIAP
ALTLTDKEYQ VMRNASFAIL REIGVDTGGS NVQFAINPKN GRMIVIEMNP 300
RVSRSSALAS KATGYPIAKV ATLLAVGFSL DEIKNDITGT PASFEPVIDY IVTKIPRFTF
360 EKFPGANTTL GTAMKSVGEV MAIGRTFKES IQKALCSLER SLSGFDRVKF
EDRNDLVFKI 420 RNANEKRLLY VAQAFREGFS VEELYELCKI DPWFLTQIKE
IVDFEEQIDM DILNNKALLR 480 KAKTMGFSDK MIALLVNLKD NLELSQNDIY
YVRMKQKIIA EFSEVDTCAG EFEALTPYLY 540 SSINVSELTQ SKNDAKDKKE
KKVMIIGGGP NRIGQGIEFD YACVHASFAL KDMGIKTIMY 600 NCNPETVSTD
YDTSDILYFE PIDFEHLRAV IEREKPDGVI VHFGGQTPLK FAKRLSAFGA 660
KIIGTSARVI DMAEDRKKFA EFITKLGINQ PKNSTATSVE EAVLKASDIG YPVLVRPSYV
720 LGGRAMRVVN DEAELRLYMQ EAVDVSDKSP VLIDQFLDNA TEIDVDAICD
GKDVYVAGIM 780 EHIEEAGIHS GDSACSLPPC NIDEKMQEFI AQKTADIALN
LGVVGLLNIQ FALHNNELYM 840 IEVNPRASRT IPFVSKATGI PLAKVATRVM
WQGNLKEALK FYDTFKVVNF DTKILRPKTP 900 KYMSVKEAVF PFAKLSGSDL
ELGPEMRSTG EVMGISKDFA NSYAKSQIAS FNHLPEQGVV 960 FISLKDKDKK
YTKKIAAEYV KLGFKLMATG GTCKEILESG FECELVHKIS EGRPNVEDKL 1020
KNGEIHLVIN TSDSHSFKGD TKKIRENIIR FKIPYFTNLR SALAGAKSIK AIQSKSCLDV
1080 KSLQEWLKS 1089 SEQ ID NO: 25 MKNITLTKIP IGEGKEPCLN SKKIVLSLAT
ISFLASCANA KLNSEIKTYD EVNKNVKTRS 60 ASVYSPQAKI NTTINSLHNQ
QVTITGNGTS NSLTIGSSGT LGSIGNTGKI IYAHANGSNT 120 LTLANLTNNR
TINGKIGIEN NGNFTGTIAV NTFENTGQIN GQIYMGIWGN NSGTLNIDKF 180
DNSGTIIDNN KGVFEGKNTN IQTFNNSGFI SANKGVDIGN IGTIKNFNNN GTIQGSEVGV
240 AINTKIDTFT NNGFINSPGS GQWNNGIWIS SNATIEKLVN NGTIKGGHSA
IMVTSQHIKT 300 VENTGIIHAE GEWGSSILLE YGGFIEHIIN TGTISNNNVG
IGSAYGVFGT LTIKDGGMVY 360 GKYSAIGVGR SQTLGDLYID GRSNNGTVSG
IYSEEHGILL ENNSRTQKIE LKNGGIIKGN 420 IDGIRLINSA SLSGEMILSG
EGSRVEGGRG VGILNRSGKI EGSIKVEDGA TVTATSNRAI 480 ANSGSGSITG
GITVSGKNTK LEGNIINTGN ASIGSDIKIE GGAKVEGGLV NQGNGSISGS 540
VQVSGGSSID SITNEGNGAI SGSITVYKDS KLDSITNTST SSTGISGSIT NNSDNKLEIS
600 NSGNIGGKIE STGSADMVIS NSNGGTISGG ISSSGSGSTS ISNSQGSTIN
NGITVSGSAQ 660 VEISNQGSVG KDENGNTVTN NGSGSVGIKD WLVSTDKNTG
KLNTVVIGGS RAFNVKVENI 720 TVDQSNVDLE ELNDINNIIS GVNQNNIGNI
GTNGSGEISL SFDPITGKLT TDFNLNASIS 780 GATFRSLIST TSRRSTFIDN
VMGNSMQSFA LASSSKSQSI AMSEKGNLYA DASDYIKSDL 840 NNGSYGSNKE
HSLFILPYTS SQNVELSLNE ESKGHTKGTI IGYSTLKDSG IYGVYAGYED 900
TKMGSTYFDI NNRTYYAGLK YFNTLFTTEK GQEVYIKAQG KAALIKNDLT EKIGNNEAKA
960 EPNSYAYGVN TALGMNFISN KDIFSPEIGL AYEGGYTEAF SMKDTIGQAT
VKGGERTYAN 1020 YLNLFSTKTS LTWFRDWLPN LKTSVELGAK FNINPKVEAE
ARFGNIKVSD EFDLPRVQKF 1080 VSTSFIVPVN EAFYFSLNYN GMFDKDGNTH
TGFAQFNYLW 1120 SEQ ID NO: 26 MNKTALTKTY TKDIQNSCLN SKKIVLSLAT
ISFLASCTHA TLTPEIKTYE ETNRHAKARS 60 GLQSRNSNNE TINNLQTLTK
TISDTGNTLV IESSGTITIS NDGQQAVNFQ PNSSTSTFLN 120 KGTLIGGNNT
ASVQLGAANG NNGVSIETFN NQGIIGNGSS KFGVTVFGGG SKDNPKSIIN 180
NFSNSGTIHS NTGESIYFGN AKISSFVNSG TIKSKQGAGV NISQGTSIEN FNNTGTGIIE
240 GKRMGVNVRS TINTFVNDGL IAATNDGIQI NANVKTLINK GTIKGDAISI
RSLGGTIETL 300 TNEGIMYGKS AGIYMNRSLV KTLTNSGTIN QNNSATWSAG
IKLENGSIIE NIINTGSIRS 360 NAFGISVTGG KFGTLTIKDG GMVYGKYSAI
GVGRSQTLGD LYIDGRSNNG TVSGIYSEEH 420 GILLENNSRT QKIELKNGGI
IKGNIDGIRL INSASLSGEM ILSGEGSRVE GGRGVGILNR 480 SGKIEGSIKV
EDGATVTATS NRAIANSGSG SITGGITVSG KNTKLEGNII NTGNASIGSD 540
IKIEGGAKVE GGLVNQGNGS ISGSVQVSGG SSIDSITNEG NGAISGSITV YKDSKLDSIT
600 NTSTSSTGIS GSITNNSDNK LEISNSGNIG GKIESTGSAD MVISNSNGGT
ISGGISSSGS 660 GSTSISNSQG STINNGITVS GSAQVEISNQ GSVGKDENGN
TVTNNGSGSV GIKDWLVSTD 720 KNTGKLNTVV IGGSRAFNVK VENITVDQSN
VDLEELNDIN NIISGVNQNN IGNIGTNGSG 780 EISLSFDPIT GKLTTDFNLN
ASISGATFRS LISTTSRRST FIDNVMGNSM QSFALASSSK 840 SQSIAMSEKG
NLYADASDYI KSDLNNGSYG SNKEHSLFIL PYTSSQNVEL SLNEESKGHT 900
KGTIIGYSTL KDSGIYGVYA GYEDTKMGST YFDINNRTYY AGLKYFNTLF TTEKGQEVYI
960 KAQGKAALIK NDLTEKIGNN EAKAEPNSYA YGVNTALGMN FISNKDIFSP
EIGLAYEGGY 1020 TEAFSMKDTI GQATVKGGER TYANYLNLFS TKTSLTWFRD
WLPNLKTSVE LGAKFNINPK 1080 VEAEARFGNI KVSDEFDLPR VQKFVSTSFI
VPVNEAFYFS LNYNGMFDKD GNTHTGFAQF 1140 NYLW 1144 SEQ ID NO: 27
MGKIMKTMDG NEAAAYAAYA FTEVAGIYPI TPSSPMADYT DMWAAAGKKN LFGVPVKIVE
60 MQSEAGAAGS VHGSLQAGAL TTTYTASQGL LLKIPNMYKI AGQLLPCVIH
VAARSLAAQA 120 LSIFGDHQDI YAARQIGFAM LCSHSVQETM DLAGVAHLAA
IKGRVPFLHF FDGFRTSHEI 180 QKVEVMDYAH FDRLLDREAL LEFRNNALNP
ENPKTRGTAQ NDDIYFQTRE VSNRFYDALP 240 DVVNEYMQEI SKITGREYKP
FTYYGHKEPE CVIVAMGSVT QALEEVVDYL NAKGEKVGIL 300 KVYLYRPFSL
KYFFDVMPKS VKKIAVLDRT KEPGSLGEPL YLDVKSAFYG RENAPVIVGG 360
RYGLSSKDVD PAQMIAVFEN LKLDNPKDGF TVGIIDDVTH TSLSTGEKIS LGDESTIECL
420 FYGLGADGTV GANKNSIKII GDKTDFYAQA YFAYDSKKSG GYTRSHLRFS
KKPIRSTYLV 480 STPHFIACSV AAYLEIYDVL AGIRKGGTFL LNSIWNAEET
IRQLPDAVKK TLAEKEVNFY 540 IINATKLARD IGLGNRTNTI MQSAFFKLAK
IIPYEDAQKY MKELAYKSYS KKGDAIVEMN 600 YKAIDVGADG LVKVEVDPNW
KNLELKEKEQ TNAYKGTEFV EKIVKPMNAA KGDDLPVSAF 660 LGYEDGSFEH
GTTEYEKRGV GVMVPRWIEA NCIQCNQCAS VCPHAVIRPF LINDEEMANA 720
PRGVKDHALE AKGTKGEKLS FKIQVSPLDC TGCELCVHEC PTKEKSLVMV PLQEEMDFGE
780 QENADYLFKE ITYKDDILNK ETTKGAQFAQ PLFEFHGACP GCGETPYITL
ITRLFGERMI 840 VANATGCSSI YGGSAPSTPY RKSVKNGHGP AWGNSLFEDN
AEFGLGMKIA TENTRHRIEH 900 IMNESMQEVP NALSALFKDW IANKDNGAMS
VEIKDKMIPI LEQNKNIKAV QDILELKQYL 960 SKKSHWIFGG DGWAYDIGYG
GLDHVLASGE NVNILVLDTE VYSNTGGQSS KSSRTGAVAQ 1020 FAAAGKPIQK
KDLGQIAMTY GYIFVAQVNS TANYTHLIKA ITAAEAYDGP SLVICYSPCI 1080
AHGIKGGLGY SGEQGELATK CGYWPLYTFD PRLEEQGKNP LTLTGKEPDW DLYEQFLMNE
1140 VRYNSLKKAN PEHAAELFER NKKDAQRRYR QLKRIAMADY SNEVES 1186 SEQ ID
NO: 28 MCDMLDNKLG NRLRVDFSNI SKQIEIPNLL QLQKKSFDYF LNLDNGESGI
EKVFKSIFPI 60 HDPQNRLSLE YVSSEIGKPK YTIRECMERG LTYSVNLKMK
IRLTLHEKDE KTGEKVGVKD 120 IKEQEIYIRE IPLMTDRVSF IINGVERVVV
NQLHRSPGVI FKEEESSTVA NKLVYTAQII 180 PDRGSWLYFE YDAKDVLYVR
INKRRKVPVT MLFRALGYKK QDIIKLFYPI QTIHVKKDKF 240 LTEFNPNDFM
DRIEYDIKDE KGKIVHQAGK RLTKKKAEQL IKDGLKWIEY PVEILLNRYL 300
ANPIIDKESG EVLFDSLTLL DESKLAKIKE QKSFDIANDL ANGVDAAIIN SFAQDGETLK
360 LLKQSENIDD ENDLAAIRIY KVMRPGEPVV KDAAKAFVND LFFNPERYDL
TKVGRMKMNH 420 KLGLEVPEYV TVLTNEDIIK TAKYLIKVKN GKGHIDDRDH
LGNRRIRSIG ELLANELHLG 480 LAKMQKAIRD KFTSLNADLD KVMPYDLINP
KMITTTIIEF FTGGQLSQFM DQTNPLSEVT 540 HKRRLSALGE GGLVKERAGF
EVRDVHATHY GRICPVETPE GQNIGLINTL STYAKVNELG 600 FVEAPYRKVV
NGKVTNEVVY LTATQEEGLF IAPASTKVDA KGNIVEEFVE ARQDGETILA 660
RREEVQLIDL CSGMVVGVAA SLIPFLEHDD ANRALMGSNM QRQAVPLLTA SAPIVGTGME
720 QIIARDAWEA VKAKRGGVVE KVDNKSIFIL GEDDKGPFID HYTMEKNLRT
NQNTNYIQHP 780 IVKKGDIVKA GQIIADGPSM DQGELAIGKN ALIAFMPWNG
YNYEDAIVVS ERIIREDTFT 840 SVHIYEKEIE ARELKDGIEE ITKDIPNVKE
EDVAHLDESG IAKIGTHIKP GMILVGKVSP 900 KGEVKPTPEE RLLRAIFGEK
AGHVVNKSLY ATASLEGVVV DVKIFTKKGY EKDDRAIKSY 960 DKEKMALEKE
HHDRLLMMDR EEMLRVCALL SKASLNSDQK IGDKNYKKGQ TADISELEKI 1020
NRFTLTTLIK AYSKEIQKEY DDLKNHFQNE KKKLKAEHDE KLEILEKDDI LPSGVIKLVK
1080 VYIATKRKLK VGDKMAGRHG NKGIVSTIVP EVDMPYLPNG KSVDIALNPL
GVPSRMNIGQ 1140 ILESHLGLVG LRLGDQIQEI FDRKQKDFLK ELRAKILEIC
SIPRLANEKE FIKSLSDEEL 1200 LNYARDWSKG VKFSTPVFEG VNIEEFSKLF
KMAKIDMDGK TELYDGRTGE KIAERVHVGC 1260 MYMLKLHHLV DEKVHARSTG
PYSLVTQQPV GGKALFGGQR FGEMEVWALE AYGAAHTLRE 1320 MLTIKSDDVE
GRFSAYKALT KGENVPATGI PETFFVLTNE LKSLALDVEI FDKDEDNE 1378 SEQ ID
NO: 29 MDLENILENN QSIGLYHPKN EHDACGIAAV ANIRGIASYK VICDALEILM
NLEHRGGTGA 60 EENSGDGAGI LIQIPHDFFK TQELGFELPK KGDYAVAQMF
LSPNTDAKEE AKEIFLQGLK 120 DKKLEFLGFR EVPFNPSDIG ASALKAMPYF
LQAFVKKPSK ISAGLEFERV LYSTRRLIEK 180 RAINVPKFYF SSFSSRTIVY
KGMLLSTQLS DFYLDFKDVN MKSAIALVHS RFSTNTFPSW 240 ERAHPNRYMV
HNGEINTIRG NVDSIRAREG LMQSEYFENL DEIFPIIAKL SSDSAMFDNT 300
LEFLALNGRT LEEAFMMMVP EPWHKNENME SKKRAFYEYH SLLMEPWDGP AAIVFTDGVI
360 MGASLDRNGF RPSRYYLTKD DMLILSSETG ALKLDEKNIK AKKRLEPGKL
LLVDTARGRV 420 IADNEIKEHY ANAKPYKKWL KNLVELEKQK SGVYKHQFLK
EDEVLKLQKA FGWSYDELKM 480 SVAAMAQNGK EAIAAMGVDT PLAILSKTYQ
PLYNYFKQLF AQVTNPPLDA IREEIVTSTR 540 IYLGSEGNLL KPDENNAKRV
KIALPVISNE ELFEVKALNK FQVKEFSILY DYSKKTLEKA 600 LDELCVKIED
EVKKGVSIII LSDKGVDEKN AYIPALLAVS GVHNHLVRKN LRTHTSLIIE 660
SGEPREIHHF ACLLGYGATV INPYLVYESI QKLIANKDLN LSYEKAVENF IKASSSGIVK
720 IASKMGVSTL QSYNGSALFE CLGLSSKVID KYFTSTTSRI EGMDLEDFEK
ELIALHKHAF 780 NDTHKALDSK GIHGFRSAKE EHLIDPLVIF NLQQACRNKD
YKSFKKYSAL VDEKQVNLRS 840 LMEFDFSEAI SIDKVESVES IVKRFRTGAM
SYGSISKEAH ECLAQAMNKI GAKSNSGEGG 900 EDEERYEIKE GVDKNSAIKQ
VASGRFGVDL NYLSHAKEIQ IKVAQGAKPG EGGQLMGFKV 960 YPWIAKARHS
TAGVTLISPP PHHDIYSIED LAQLIYDLKH ANKDAKISVK LVSENGIGTV 1020
AAGVAKAGAN LILVSGYDGG TGASPRTSIP HAGIPWELGL AETHQTLILN KLRDRVRLET
1080 DGKLMNGRDL AIAALLGAEE FGFATAPLIV LGCTMMRVCH LNTCPFGIAT
QDTELRDRFK 1140 GKVDDVINFM YFIAEELREY MARLGFERLD DMIGRVDKLR
QKSVQGKAGK LNLDKILKSL 1200 PTYNRTAVHF KDYKDNKLEK TIDYRILLPL
CKNAVEKKEP IKLSLEVGNQ SRTFATMLSS 1260 EILKTYGKDA LDEDSIHIKA
IGNAGNSFGA FLLKGIKLEI IGDSNDYLGK GLSGGKIIAK 1320 ISNEATFSPE
ENIIAGNACL YGATKGEVYL DGIAGERFCV RNSGALAVVL GTGVHGCEYM 1380
TGGQVVVLGD VGANFAAGMS GGVVYIFGRH NEAHVNTELV DIKDLNAKDE KELKAVIEKH
1440 ITYTDSKKAK DILEKFDKKD FFKVMPRDYE KMLKMLDLCK NEKDPNLAAF LKITQK
1496 SEQ ID NO: 30 MSKFKVIEIK EDARPRDFEA FQLRLASPEK IKSWSYGEVK
KPETINYRTL KPERDGLFCA 60 KIFGPIRDYE CLCGKYKKMR FKGVKCEKCG
VEVANSKVRR SRMGHIELVT PVAHIWYVNS 120 LPSRIGTLLG VKMKDLERVL
YYEAYIVENP GDAFYDNEST KKVEYCDVLN EEQYQNLMQR 180 YENSGFKARM
GGEVVRDLLA NLDLVALLNQ LKEEMGATNS EAKKKTIIKR LKVVENFLNS 240
NLNANTDSDE AVPNRPEWMM ITNLPVLPPD LRPLVALDGG KFAVSDVNDL YRRVINRNTR
300 LKKLMELDAP EIIIRNEKRM LQEAVDALFD NGRRANAVKG ANKRPLKSLS
EIIKGKQGRF 360 RQNLLGKRVD FSGRSVIVVG PKLRMDQCGL PKKMALELFK
PHLLAKLEEK GYATTVKQAK 420 KMIENKTNEV WECLEEVVKG HPVMLNRAPT
LHKLSIQAFH PVLVEGKAIQ LHPLVCAAFN 480 ADFDGDQMAV HVPLSQEAIA
ECKVLMLSSM NILLPASGKS VTVPSQDMVL GIYYLSLEKA 540 GAKGSHKICT
GIDEVMMALE SKCLDIHASI QTMVDGRKIT TTAGRLIVKS ILPDFVPENS 600
WNKVLKKKDI AALVDYVYKQ GGLEITASFL DRLKNLGFEY ATKAGISISI ADIIVPNDKQ
660 KAIDEAKKQV REIQNSYNLG LITSGERYNK IIDIWKSTNN VLSKEMMKLV
EKDKEGFNSI 720 YMMADSGARG SAAQISQLAA MRGLMTKPDG SIIETPIISN
FREGLNVLEY FISTHGARKG 780 LADTALKTAN AGYLTRKLID VAQNVKITIE
DCGTHEGVEI NEITADSSII ETLEERILGR 840 VLAEDVIDPI TNSVLFAEGT
LMDEEKAKIL GESGIKSVNI RTPITCKAKK GICAKCYGIN 900 LGEGKLVKPG
EAVGIISAQS IGEPGTQLTL RTFHSGGTAS TDLQDRQVSA QKEGFIRFYN 960
LKTYKNKEGK NIVANRRNAA VLLVEPKIKT PFKGVINIEN IHEDVIVSIK DKKQEVKYIL
1020 RKYDLAKPNE LAGVSGSIDG KLYLPYQSGM QVEENESIVE VIKEGWNVPN
RIPFASEILV 1080 EDGEPVVQNI KAGEKGTLKF YILKGDGLDR VKNVKKGDIV
KEKGFFVVIA DENDREAKRH 1140 YIPRESKIEF NDSEKIDDAN TIIASAPKKE
RKVIAEWDAY NNTIIAEIDG VVSFEDIEAG 1200 YSADEQIDEA TGKRSLVINE
YLPSGVRPTL VIAGKGDKAV RYHLEPKTVI FVHDGDKIAQ 1260 ADILAKTPKA
AAKSKDITGG LPRVSELFEA RKPKNAAVIA EIDGVVRFDK PLRSKERIII 1320
QAEDGTSAEY LIDKSKHIQV RDGEFIHAGE KLTDGVVSSH DVLKILGEKA LHYYLISEIQ
1380 QVYRGQGVVI SDKHIEVIVS QMLRQVKVVD SGHTKFIEGD LVSRRKFREE
NERIIRMGGE 1440 PAIAEPVLLG VTRAAIGSDS VISAASFQET TKVLTEASIA
GKFDYLEDLK ENVILGRMIP 1500 VGTGLYGEQN LKLKEQE 1517
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Sequence CWU 1
1
91151PRTCampylobacter jejuni 1Met Ala Tyr Glu Asp Glu Glu Asp Leu
Asn Tyr Asp Asp Tyr Glu Asn 1 5 10 15 Glu Asp Glu Glu Tyr Pro Gln
Asn His His Lys Asn Tyr Asn Tyr Asp 20 25 30 Asp Asp Asp Tyr Glu
Tyr Asp Asp Asp Asn Asn Asp Asp Asp Phe Tyr 35 40 45 Glu Met Asp 50
265PRTCampylobacter jejuni 2Met Ile Asn Pro Ile Gln Gln Ser Tyr Val
Ala Asn Thr Ala Leu Asn 1 5 10 15 Thr Asn Arg Ile Asp Lys Glu Thr
Lys Thr Asn Asp Thr Gln Lys Thr 20 25 30 Glu Asn Asp Lys Ala Ser
Lys Ile Ala Glu Gln Ile Lys Asn Gly Thr 35 40 45 Tyr Lys Ile Asp
Thr Lys Ala Thr Ala Ala Ala Ile Ala Asp Ser Leu 50 55 60 Ile 65
3116PRTCampylobacter jejuni 3Met Lys Lys Ile Leu Ile Leu Leu Thr
Leu Cys Ala Phe Ala Phe Gly 1 5 10 15 Ala Ser Glu Cys Asp Arg Lys
Ile Asp Arg Ile Asn Lys Glu Ile Ser 20 25 30 Phe Ser Lys Ala His
Asn Asp Thr Ala Arg Thr Leu Ser Leu Glu Leu 35 40 45 Ala Leu Lys
Gln Val Gln Asn Asp Cys Ala Lys Asp Pro Met Phe Tyr 50 55 60 Asp
Lys Lys Leu Glu Ala Lys Lys Leu Lys Glu Gln Glu Val Glu Lys 65 70
75 80 Ile Glu Lys Glu Leu Asp Ala Leu Lys Glu Gln Lys Asp Tyr Met
Ser 85 90 95 Lys Ala Glu Tyr Lys Ala Lys Lys Glu Ala Leu Lys Glu
Gln Lys Glu 100 105 110 Lys Ile Lys Lys 115 4138PRTCampylobacter
jejuni 4Met Ser Phe Gly Glu Ile Ile Val Ile Leu Val Val Ala Ile Leu
Val 1 5 10 15 Leu Gly Pro Asp Lys Leu Pro Glu Ala Ile Val Gln Ile
Ala Lys Ile 20 25 30 Leu Lys Ala Val Lys Arg Asn Ile Asp Asp Ala
Lys Ser Ser Ile Glu 35 40 45 Lys Glu Ile Arg Ile Asn Asp Leu Lys
Glu Glu Ala Lys Lys Tyr Lys 50 55 60 Asp Glu Phe Ser Ser Thr Asn
Glu Asn Ile Arg Lys Lys Leu Ser Phe 65 70 75 80 Glu Glu Phe Asp Asp
Leu Lys Arg Asp Ile Leu Asp Lys Thr Lys Val 85 90 95 Asp Leu Thr
Phe Asp Ser Arg Asp Asp Lys Val Lys Asn Asn Leu Ser 100 105 110 Gly
Gln Asn Leu Asn Thr Glu Glu Lys Pro Asn Leu Ser Lys Leu Glu 115 120
125 Thr Gln Asp Lys Asn Gly Lys Ile Asn Val 130 135
5141PRTCampylobacter jejuni 5Met Gln Lys Ala Lys Ile Leu Ile Ala
Leu Ser Phe Phe Leu Leu Val 1 5 10 15 Leu Ser Ala Cys Ser Asn Asp
Glu Lys Asn Ile Ser Lys Thr Gln Asn 20 25 30 Thr Asp Gln Glu Val
Val Gln Ile Glu Gln Asn Asp Glu Lys Thr Glu 35 40 45 Leu Ser Asp
Ser Asn Leu Pro Leu Pro Val Asp Asp Glu Ala Gln Ser 50 55 60 Ser
Asn Asp Glu His Glu Val Asn Pro Ser Ile Ile Asn Ser Leu Tyr 65 70
75 80 Lys Gln Lys Cys Ala Thr Cys His Gly Glu Lys Gly Glu Leu Lys
Pro 85 90 95 Lys Asn Ser Thr Ala Ile Lys Thr Leu Ser Asn Lys Ile
Phe Ile Gln 100 105 110 Lys Ile Lys Thr Ile Lys Asp Lys Asn His Ser
Phe Leu Ser Asp Glu 115 120 125 Gln Ile Gln Asn Leu Ala Asp Phe Ile
Asn Lys Gly Lys 130 135 140 6144PRTCampylobacter jejuni 6Met Arg
Arg Leu Ser Ile Leu Leu Ala Ile Leu Ile Val Ile Asn Ile 1 5 10 15
Thr Ala Cys Asp Ser Lys Thr Glu Asn Tyr Tyr Lys Asn Leu Pro Ser 20
25 30 Glu Ala Lys Glu Lys Ala Lys Glu Cys Lys Glu Ser Gly Thr Leu
Ser 35 40 45 Glu Asp Cys Ile Asn Ala Leu Lys Val Gly Val Lys Pro
Thr Asn Glu 50 55 60 Glu Gly Lys Tyr Ser Pro Asn Thr Pro Lys Lys
Ser Asp Asn Gln Ile 65 70 75 80 Leu Glu Ala Leu Lys Gln Asn Asp Leu
Lys Lys Glu Lys Thr Thr Lys 85 90 95 Asp Ile Asn Gln Ser Ser Glu
Asn Asn Glu Ser Ile Ile Ile Pro Pro 100 105 110 Ile Ala Glu Thr Pro
Ser Glu Ile Tyr Pro Ser Lys Thr Thr Glu Asn 115 120 125 Asn Gln Ser
Ser Ile Phe Ser Asp Asp Val Asn Met Thr Gln Glu Lys 130 135 140
7174PRTCampylobacter jejuni 7Met Met Lys Lys Lys Leu Val Leu Leu
Gly Ser Ala Ala Val Val Phe 1 5 10 15 Phe Ala Ala Cys Ala Met Asn
Ser Gly Val Ser Ser Glu Gln Ile Gly 20 25 30 Leu Arg Lys Ala Ser
Leu Glu Asn Glu Asn Lys Val Asn Leu Val Glu 35 40 45 Ala Asn Phe
Thr Thr Leu Gln Pro Gly Glu Ser Thr Arg Phe Glu Arg 50 55 60 Ser
Tyr Glu Asn Ala Pro Pro Leu Ile Pro His Ala Ile Glu Asp Leu 65 70
75 80 Leu Pro Ile Thr Lys Asp Asn Asn Met Cys Leu Ser Cys His Asp
Lys 85 90 95 Ala Ile Ala Ala Asp Ala Gly Ala Thr Pro Leu Pro Ala
Ser His Tyr 100 105 110 Tyr Asp Phe Arg His Asn Lys Thr Thr Gly Asp
Met Ile Ser Asp Ser 115 120 125 Arg Phe Asn Cys Thr Gln Cys His Val
Pro Gln Ser Asp Ala Lys Pro 130 135 140 Leu Val Gly Asn Ser Phe Lys
Pro Glu Phe Lys Asn Glu Gln Leu Lys 145 150 155 160 Ser Arg Ser Asn
Leu Ile Asp Val Ile Asn Glu Gly Val Lys 165 170
8201PRTCampylobacter jejuni 8Met Lys Lys Ile Lys Lys Ile Ile Gln
Ile Gly Met Ile Gly Gly Leu 1 5 10 15 Ala Ala Val Ala Gly Gly Ala
Leu Ala Gly Cys Gly Ser Asn Asn Asp 20 25 30 Asn Ala Asp Thr Leu
Asn Gln Ala Ala Asn Ala Gln Gly Ala Phe Val 35 40 45 Ile Ile Glu
Glu Thr Ala Pro Gly Gln Tyr Lys Ile Lys Asp Gln Tyr 50 55 60 Pro
Ser Asp Glu Thr Arg Val Val Leu Lys Asp Leu Asn Gly Thr Glu 65 70
75 80 Arg Ile Leu Ser Lys Glu Glu Met Asp Ala Leu Ile Lys Glu Glu
Ala 85 90 95 Ala Lys Ile Asp Asn Gly Thr Ser Asn Leu Thr Lys Asp
Asn Gly Gln 100 105 110 Ile Ser Ser Gly Gly Leu Ser Leu Gly Glu Thr
Leu Leu Ala Ser Ala 115 120 125 Ala Gly Ala Ile Leu Gly Ser Trp Ile
Gly Ser Lys Leu Phe Asn Asn 130 135 140 Gln Asn Phe Ala Asn Gln Gln
Arg Gly Ala Phe Ser Asn Gln Ser Ala 145 150 155 160 Tyr Gln Arg Ser
Val Asn Ser Phe Asn Lys Ala Gly Thr Thr Ser Ser 165 170 175 Ala Ser
Ser Ala Lys Lys Ser Gly Phe Phe Gly Gly Gly Ser Lys Ala 180 185 190
Thr Ser Ser Ser Ser Ser Phe Gly Ser 195 200 9210PRTCampylobacter
jejuni 9Met Thr Lys Phe Leu Ser Ile Cys Ser Leu Ile Ala Met Leu Leu
Ser 1 5 10 15 Gly Cys Gly Ser Asp Phe Pro Gly Gln Pro Ser Asp Val
Ala Arg Val 20 25 30 Gln Gln Asn Lys Tyr Pro Asn Gly Asn Leu Lys
Lys Glu Ile Pro Tyr 35 40 45 Asn Lys Asp Ser Arg Ile His Gly Leu
Lys Arg Ala Phe Tyr Asp Asn 50 55 60 Gly Gln Leu Arg Ala Glu Glu
Asn Tyr Lys Asn Gly Lys Lys Asp Gly 65 70 75 80 Ile Ser Arg Glu Tyr
Ser Arg Asn Gly Gln Leu Leu Glu Glu Val His 85 90 95 Phe Lys Asp
Asn Arg Gly Tyr Gly Asp Phe Ala Ser Tyr Tyr Glu Asn 100 105 110 Gly
Asn Met Arg Ala Lys Gly Lys Leu Leu Gly Tyr Asn Glu Asp Gly 115 120
125 Met Pro Glu Phe Glu Gly Asn Tyr Lys Glu Tyr Tyr Glu Asn Gly Thr
130 135 140 Leu Met Cys Asp Tyr Asn Phe Asp Asn Lys Gly Lys Phe Asp
Gly Val 145 150 155 160 Gln Lys Arg Tyr Asp Glu Asn Gly Ala Leu Glu
Asp Glu Glu Asn Tyr 165 170 175 Lys Asn Gly Leu Lys Asn Gly Val Phe
Arg Glu Tyr Lys Lys Gly Glu 180 185 190 Ile Val Arg Glu Glu Glu Tyr
Lys Asn Gly Ile Leu Val Ala Lys Pro 195 200 205 Lys Asn 210
10244PRTCampylobacter jejuni 10Met Lys Lys Ile Phe Leu Ser Val Phe
Leu Val Leu Ser Leu Asn Ala 1 5 10 15 Gln Asn Leu Glu Ile Asp Lys
Ile Arg Thr Asp Leu Tyr Ser Lys Ser 20 25 30 Gly Ala Asn Val Leu
Lys Lys Val Glu Ile Ser Leu Glu Phe Asp Gly 35 40 45 Asn Asn Leu
Lys Glu Asn Glu Asn Lys Leu Ile Asp Ala Val Asn Thr 50 55 60 Val
Ile Ser Gly Phe Phe Tyr Glu Asp Ile Phe Thr Glu Ile Gly Lys 65 70
75 80 Asn Asn Phe Lys Lys Thr Leu Glu Lys Phe Leu Asp Lys Lys Tyr
Lys 85 90 95 Ile Lys Leu Asp Asp Ile Tyr Ile Ile Ser Leu Ser Gly
Val Glu Lys 100 105 110 Phe Asp Leu Glu Glu Phe Lys Arg Phe Leu Glu
Ser Thr Glu Ala Lys 115 120 125 Glu Lys Gly Met Gly Ser Glu Val Lys
Lys Ala Leu Glu Asn Leu Glu 130 135 140 Val Pro Lys Thr Gln Val Pro
Ser Val Glu Lys Ile Pro Thr Pro Ser 145 150 155 160 Val Pro Asn Leu
Glu Val Lys Gln Val Glu Gln Leu Phe Lys Asp Pro 165 170 175 Asp Glu
Glu Asn Lys Asn Asp Asn Gly Glu Ile Asn Ile Asp Asn Leu 180 185 190
Asn Thr Pro Lys Met Thr Pro Asp Ile Glu Glu Lys Ile Lys Arg Asp 195
200 205 Leu Ile Ala Asn Pro Pro Gln Ile Phe Lys Glu Asn Asn Ala Ser
Lys 210 215 220 Pro Tyr His Leu Pro Gln Thr Gly Tyr Asp Ile Lys Leu
Asp Glu Asn 225 230 235 240 Ser Thr Gln Asn 11259PRTCampylobacter
jejuni 11Met Lys Asn Tyr Gly Leu Ser Asn Leu Asn Ser Phe Leu Leu
Ala Leu 1 5 10 15 Ala Ile Tyr Ile Ser Ile Val Ile Leu Val Phe Phe
Arg Leu Val Ser 20 25 30 Glu Val Glu Pro Ala Ile Gln Tyr Thr Asp
Ile Lys Asp Ser Phe Val 35 40 45 Asp Ile Glu Leu Ala Glu Pro Ser
Lys Gln Val Ile Thr Gln Ser Asn 50 55 60 Thr Pro Lys Glu Ile Gln
Lys Pro Thr Glu Gln Ile Asp Ile Glu Lys 65 70 75 80 Leu Phe Ala Gln
Thr Thr Asn Lys Thr Val Lys Thr Glu Asp Ile Asp 85 90 95 Gln Lys
Ala Ser Asn Phe Asn Glu Leu Phe Gly Asn Ile Lys Glu Ile 100 105 110
Gln Glu Glu Lys Thr Thr Lys Ile Gln Ser Ser Ala Lys Ser Gly Ile 115
120 125 Ser Ser Ala Pro Lys Pro Gln Ala Ser Glu Leu Val Lys Gln Leu
Asn 130 135 140 Asp Ser Leu Leu Gln Glu Glu Ser Ser Thr Gln Gly Glu
Ser Thr Lys 145 150 155 160 Ala Gln Lys Ile Gly Ile Tyr Asp Glu Phe
Leu Gly Lys Val Val Arg 165 170 175 Ile Ile Thr Gln Arg Trp Thr Gln
Tyr Tyr Pro Asn Ser Glu Lys Ile 180 185 190 Ser Val Lys Val Lys Ile
Phe Ile Asp Glu Asn Gly Lys Phe Gly Tyr 195 200 205 Thr Ser Val Glu
Lys Ser Gly Asn Pro Leu Tyr Asp Ala Lys Val Ala 210 215 220 Glu Phe
Leu Glu Ser Gln Lys Gly Lys Phe Ile Thr Tyr Pro Pro Gln 225 230 235
240 Asn Lys Asn Ile Ser Ile Thr Met Asn Leu Arg Asp Glu Val Lys Val
245 250 255 Lys Asn Asp 12273PRTCampylobacter jejuni 12Met Lys Lys
Phe Ser Leu Val Ala Ala Thr Leu Ile Ala Gly Val Val 1 5 10 15 Leu
Asn Val Asn Ala Ala Thr Val Ala Thr Val Asn Gly Lys Ser Ile 20 25
30 Ser Asp Thr Glu Val Ser Glu Phe Phe Ala Pro Met Leu Arg Gly Gln
35 40 45 Asp Phe Lys Thr Leu Pro Asp Asn Gln Lys Lys Ala Leu Ile
Gln Gln 50 55 60 Tyr Ile Met Gln Asp Leu Ile Leu Gln Asp Ala Lys
Lys Gln Asn Leu 65 70 75 80 Glu Lys Asp Pro Leu Tyr Thr Lys Glu Leu
Asp Arg Ala Lys Asp Ala 85 90 95 Ile Leu Val Asn Val Tyr Gln Glu
Lys Ile Leu Asn Thr Ile Lys Ile 100 105 110 Asp Ala Ala Lys Val Lys
Ala Phe Tyr Asp Gln Asn Lys Asp Lys Tyr 115 120 125 Val Lys Pro Ala
Arg Val Gln Ala Lys His Ile Leu Val Ala Thr Glu 130 135 140 Lys Glu
Ala Lys Asp Ile Ile Asn Glu Leu Lys Gly Leu Lys Gly Lys 145 150 155
160 Glu Leu Asp Ala Lys Phe Ser Glu Leu Ala Lys Glu Lys Ser Ile Asp
165 170 175 Pro Gly Ser Lys Asn Gln Gly Gly Glu Leu Gly Trp Phe Asp
Gln Ser 180 185 190 Thr Met Val Lys Pro Phe Thr Asp Ala Ala Phe Ala
Leu Lys Asn Gly 195 200 205 Thr Ile Thr Thr Thr Pro Val Lys Thr Asn
Phe Gly Tyr His Val Ile 210 215 220 Leu Lys Glu Asn Ser Gln Ala Lys
Gly Gln Ile Lys Phe Asp Glu Val 225 230 235 240 Lys Gln Gly Ile Glu
Asn Gly Leu Lys Phe Glu Glu Phe Lys Lys Val 245 250 255 Ile Asn Gln
Lys Gly Gln Asp Leu Leu Asn Ser Ala Lys Val Glu Tyr 260 265 270 Lys
13278PRTCampylobacter jejuni 13Met Glu Asn Gln Lys Asn Glu Phe Asp
Asp Ile Ile Leu Glu Lys Ser 1 5 10 15 Asn Lys Ser Glu Lys Val Lys
Lys Ile Leu Leu Arg Val Ile Ala Leu 20 25 30 Val Ile Leu Phe Leu
Ala Ile Met Ile Val Met Lys Leu Ile Asn Gly 35 40 45 Ser Gly Asp
Glu Asn Thr Gln Asn Gln Ser Val Leu Pro Ser Glu Pro 50 55 60 Ile
Ala Thr Gln Asp Asn Asn Asn Asp Thr Ser Phe Glu Ser Met Pro 65 70
75 80 Ile Thr Asp Asn Thr Ser Ala Glu Asp Gln Phe Glu Ala Leu Arg
Lys 85 90 95 Gln Phe Gln Asp Glu Gln Asn Thr Thr Gln Asn Thr Thr
Thr Ser Ser 100 105 110 Ser Asn Asn Asn Asp Thr Thr Asn Phe Ala Met
Pro Asp Gln Glu Val 115 120 125 Pro Ala Glu Pro Thr Ala Thr Thr Ser
Ala Asn Thr Thr Pro Gln Ala 130 135 140 Ser Thr Pro Lys Gln Glu Val
Thr Gln Thr Ala Lys Ser Lys Glu Glu 145 150 155 160 Ala Lys Lys Gln
Thr Ala Val Lys Lys Glu Lys Glu Ser Ala Lys Gln 165 170 175 Thr Pro
Lys Lys Glu Gln Asn Ala Asn Asp Leu Phe Lys Asn Val Asp 180 185 190
Ala Lys Pro Val His Pro Ser Gly Leu Ala Ser Gly Ile Tyr Val Gln 195
200 205 Ile Phe Ser Val Ser Asn Leu Asp Gln Lys Ser Lys Glu Leu Ala
Ser 210 215 220 Val Lys Gln Lys Gly Tyr Asp Tyr Lys Leu Tyr Lys Thr
Thr Val Gly 225 230 235
240 Ser Lys Glu Ile Thr Lys Val Leu Ile Gly Pro Phe Glu Lys Ala Asp
245 250 255 Ile Ala Ala Glu Leu Ala Lys Ile Arg Lys Asp Ile Ala Lys
Asp Ala 260 265 270 Phe Ser Phe Thr Leu Lys 275
14390PRTCampylobacter jejuni 14Met Lys Lys Lys Ile Val Leu Ile Ile
Leu Ile Ala Ile Leu Gly Ser 1 5 10 15 Val Gly Ala Tyr Phe Ile Phe
Phe Asn Asn Asp Glu Lys Ile Ser Tyr 20 25 30 Leu Thr Gln Lys Ile
Gln Lys Lys Asp Ile Ser Gln Thr Ile Glu Ala 35 40 45 Val Gly Lys
Val Tyr Ala Lys Asp Gln Val Asp Val Gly Ala Gln Val 50 55 60 Ser
Gly Gln Ile Ile Lys Leu Tyr Val Asp Val Gly Thr His Val Lys 65 70
75 80 Gln Gly Asp Leu Ile Ala Gln Ile Asp Lys Asp Lys Gln Gln Asn
Asp 85 90 95 Leu Asp Ile Thr Lys Ala Gln Leu Glu Ser Ala Lys Ala
Asn Leu Glu 100 105 110 Ser Lys Lys Val Ala Leu Glu Ile Ala Asn Lys
Gln Tyr Gln Arg Glu 115 120 125 Gln Lys Leu Tyr Ala Ala Lys Ala Ser
Ser Leu Glu Asn Leu Glu Thr 130 135 140 Gln Lys Asn Asn Tyr Tyr Thr
Leu Lys Ala Asn Val Ala Glu Leu Asn 145 150 155 160 Ala Gln Val Val
Gln Leu Glu Ile Thr Leu Lys Asn Ala Gln Lys Asp 165 170 175 Leu Gly
Tyr Thr Thr Ile Thr Ala Pro Met Asp Gly Val Val Ile Asn 180 185 190
Val Ala Val Asp Glu Gly Gln Thr Val Asn Ala Asn Gln Asn Thr Pro 195
200 205 Thr Ile Val Arg Ile Ala Asn Leu Asp Glu Met Glu Val Arg Met
Glu 210 215 220 Ile Ala Glu Ala Asp Val Ser Lys Ile Lys Val Gly Thr
Glu Leu Asp 225 230 235 240 Phe Ser Leu Leu Asn Asp Pro Gln Lys Thr
Tyr His Ala Lys Ile Ala 245 250 255 Ser Ile Asp Pro Ala Asp Thr Glu
Val Ser Asp Ser Ser Thr Ser Ser 260 265 270 Ser Ser Ser Ser Ser Ser
Ser Ser Ser Ser Ser Ser Ser Asn Ala Ile 275 280 285 Tyr Tyr Tyr Ala
Lys Phe Tyr Val Ala Asn Lys Asp Asp Phe Leu Arg 290 295 300 Ile Gly
Met Ser Ile Gln Asn Glu Ile Val Val Ala Ser Ala Lys Ala 305 310 315
320 Val Leu Ala Val Pro Thr Tyr Ala Ile Lys Ser Asp Pro Lys Gly Tyr
325 330 335 Tyr Val Glu Ile Leu Glu Asn Gln Lys Ala Val Lys Lys Tyr
Val Lys 340 345 350 Leu Gly Ile Lys Asp Ser Ile Asn Thr Gln Ile Leu
Glu Gly Val Asn 355 360 365 Glu Asp Glu Glu Leu Ile Val Ser Ser Ser
Ala Asp Gly Leu Ala Pro 370 375 380 Lys Met Lys Leu Arg Phe 385 390
15542PRTCampylobacter jejuni 15Met Lys Ile Leu Leu Leu Asn Glu Asn
Pro Val Val Ser Arg Leu Val 1 5 10 15 Ser Leu Ser Ala Lys Lys Met
Ser Tyr Asp Phe Glu Glu Leu Asn Ala 20 25 30 Tyr Ser Glu Asn Leu
Gly Asn Tyr Asp Val Ile Val Val Asp Ser Asp 35 40 45 Thr Pro Ala
Pro Leu Lys Ile Leu Lys Glu Lys Cys Asp Arg Leu Ile 50 55 60 Phe
Leu Ala Pro Arg Asn Gln Asn Val Glu Asp Ile Asp Ala Gln Ile 65 70
75 80 Leu Gln Lys Pro Phe Leu Pro Thr Asp Phe Leu Asn Leu Leu Asn
Asn 85 90 95 Lys Asp Ala Asn Lys His Thr Ser Ile Asp Leu Pro Met
Leu Ser Asn 100 105 110 Asp Glu Asn Pro Tyr Ala Asp Ile Ser Leu Asp
Leu Asp Asn Leu Asn 115 120 125 Leu Asp Asp Leu Pro Asp Glu Asn Ser
Leu Asp Ile Asn Ser Glu Gly 130 135 140 Met Glu Asp Leu Ser Phe Asp
Asp Asp Ala Gln Asp Asp Asn Ala Asn 145 150 155 160 Lys Thr Leu Glu
Thr Gln Asn Leu Glu His Glu Thr Ile Lys Glu Gln 165 170 175 Thr Gln
Glu Asp Thr Gln Ile Asp Leu Asp Leu Thr Leu Glu Asp Gly 180 185 190
Glu Ser Glu Lys Glu Asp Leu Ser Gln Glu His Thr Ala Leu Asp Thr 195
200 205 Glu Pro Ser Leu Asp Glu Leu Asp Asp Lys Asn Asp Glu Asp Leu
Glu 210 215 220 Ile Lys Glu Asp Asp Lys Asn Glu Glu Ile Glu Lys Gln
Glu Leu Leu 225 230 235 240 Asp Asp Ser Lys Thr Asn Thr Leu Glu Met
Gln Glu Glu Leu Ser Glu 245 250 255 Ser Gln Asp Asp Asn Ser Asn Lys
Thr Leu Glu Thr Gln Asn Leu Glu 260 265 270 His Asp Asn Leu Glu Gln
Glu Thr Ile Lys Glu Gln Thr Gln Glu Asp 275 280 285 Thr Gln Ile Asp
Leu Asp Leu Thr Leu Glu Asp Gly Glu Ser Glu Lys 290 295 300 Glu Asp
Leu Ser Gln Glu His Thr Ala Leu Asp Thr Glu Pro Ser Leu 305 310 315
320 Asp Glu Leu Asp Asp Lys Asn Asp Glu Asp Leu Glu Asp Asn Lys Glu
325 330 335 Leu Gln Ala Asn Ile Ser Asp Phe Asp Asp Leu Pro Glu Val
Glu Glu 340 345 350 Gln Glu Lys Glu Met Asp Phe Asp Asp Leu Pro Glu
Asp Ala Glu Phe 355 360 365 Leu Gly Gln Ala Lys Tyr Asn Glu Glu Ser
Glu Glu Asn Leu Glu Glu 370 375 380 Phe Ala Pro Val Val Glu Glu Asp
Ile Gln Asp Glu Ile Asp Asp Phe 385 390 395 400 Ala Ser Asn Leu Ser
Thr Gln Asp Gln Ile Lys Glu Glu Leu Ala Gln 405 410 415 Leu Asp Glu
Leu Asp Tyr Gly Ile Asp Ser Asp Asn Ser Ser Lys Val 420 425 430 Leu
Glu Asp Phe Lys Asp Glu Pro Ile Leu Asp Asp Lys Glu Leu Gly 435 440
445 Thr Asn Glu Glu Glu Val Val Val Pro Asn Leu Asn Ile Ser Asp Phe
450 455 460 Asp Thr Leu Lys Glu Ser Asp Ile Gln Glu Ala Leu Gly Glu
Glu Ile 465 470 475 480 Leu Glu Lys Asn Glu Glu Pro Ile Val Ser Asp
Val Thr Lys Asp Asp 485 490 495 Asn Ser Glu Glu Ile Val Asn Glu Leu
Ser Gln Ser Ile Ala Gly Ala 500 505 510 Ile Thr Ser Ser Ile Lys Asp
Asp Thr Leu Lys Ala Ala Leu Lys Gly 515 520 525 Met Asn Met Asn Ile
Asn Ile Asn Ile Ser Phe Lys Glu Asp 530 535 540
16610PRTCampylobacter jejuni 16Met Lys Lys Asn Ile Leu Arg Leu Gly
Ile Val Val Leu Val Leu Leu 1 5 10 15 Ile Ala Gly Val Leu Trp Leu
Asn Asn Asp Ile Asn Gln Lys Lys Glu 20 25 30 Asp Glu Ala Asn Lys
Asn Ala Ile Ala Ala Asn Ala Asp Phe Ser Leu 35 40 45 Leu Ser Asp
Asp Asp Pro Asn Phe Glu Lys Trp Gly Lys Val Phe Pro 50 55 60 Glu
Gln Leu Lys Met Tyr Leu Thr Val Glu Lys Glu Glu Pro Lys Ala 65 70
75 80 Thr Glu Phe Gly Gly Asn Leu Ala Tyr Ser Lys Leu Ile Arg Phe
Pro 85 90 95 Gln Leu Thr Ile Leu Trp Ala Gly Tyr Pro Phe Ser Leu
Asp Phe Asn 100 105 110 Glu Glu Arg Gly His Phe Trp Val Gln Val Asp
Gln Met Lys Thr Ala 115 120 125 Arg Asn Asn Lys Asp Phe Leu Asn Ala
His Gly Leu Ala Ala Phe Lys 130 135 140 Gly Gln Pro Ala Ala Cys Met
Asn Cys His Ser Gly Trp Thr Pro Trp 145 150 155 160 Leu Ile Lys Asn
Val Ala Lys Gly Asp Phe Thr Ala Phe Asn Ser Thr 165 170 175 Asn Tyr
Trp Thr Met Ile Lys Asn Ile Pro Ala Val Asp Gly Ile Val 180 185 190
Glu Asn Ser Pro Glu His Ala Gly Pro His Gly Gly Lys Arg Met Gly 195
200 205 Val Thr Cys Ala Asp Cys His Asn Pro Asn Asp Met Ser Leu Arg
Leu 210 215 220 Thr Arg Pro Ala Ala Ile Asn Ala Leu Val Ser Arg Gly
Tyr Glu Lys 225 230 235 240 Asp Pro Val Gln Gly Val Lys Ala Thr Arg
Glu Glu Met Arg Thr Leu 245 250 255 Val Cys Ser Gln Cys His Val Glu
Tyr Tyr Phe Lys Pro Thr Gly Glu 260 265 270 Lys Val Lys Val Met Gly
Glu Thr Ile Val Asp Asp Ser Ser Lys Lys 275 280 285 Trp Trp Asn Gly
Thr Gln Lys Asn Tyr Asp Glu Tyr Glu Phe Trp Arg 290 295 300 Asp Gly
Asn Lys Val Lys Glu Ile Glu Thr Asp Gly Ile Val Leu Thr 305 310 315
320 Phe Pro Trp Ser Glu Trp Lys Lys Gly Gln Pro Phe Arg Ile Glu Met
325 330 335 Leu Asp Asp Tyr Tyr Asp Lys Val Arg Gly Val Phe Gly Ala
Asp Phe 340 345 350 Thr His Lys Leu Thr Gly Ala Gln Ile Ile Lys Ile
Gln His Pro Glu 355 360 365 Ser Glu Leu Tyr Ser Gly Gly Val His Ala
Ala Asn Gly Val Ser Cys 370 375 380 Val Asp Cys His Met Pro Tyr Val
Arg Glu Gly Ala Lys Lys Val Thr 385 390 395 400 Gln His Asn Ile Thr
Ser Pro Leu Arg Asp Ile Asn Ser Ala Cys Lys 405 410 415 Ser Cys His
Lys Gln Ser Glu Asp Tyr Leu Lys Ala Gln Val Leu Asp 420 425 430 Ile
Gln Asn Ser Val Ala His Asp Gln Arg Thr Ala Glu Tyr Ala Ile 435 440
445 Val Ser Leu Ile Met Asp Thr Lys Lys Leu Arg Asp Glu Leu Gly Asn
450 455 460 Met Glu Lys Phe Gln Ser Asp Gly Lys Ala Asp Ala Lys Lys
Ile Ser 465 470 475 480 Glu Glu Leu Lys Glu Val Leu Glu Leu His Arg
Lys Ala Gln Met Arg 485 490 495 Ala Asp Phe Val Asn Ala Glu Asn Ser
Thr Gly Phe His Asn Pro Arg 500 505 510 Glu Ala Ser Arg Met Leu Leu
Gln Ala Val Asp Met Ala Arg Met Gly 515 520 525 Gln Thr Lys Leu Val
Glu Ile Ala Ala Ala Asn Gly Ile Lys Asp Phe 530 535 540 Lys Thr Ser
Asn Leu Gly Phe Glu Asp Ile Gln Lys Phe Asn Pro Gly 545 550 555 560
Glu Leu Tyr Tyr Lys Val Asp Val Asn Asn His Lys Ala Gly Glu Arg 565
570 575 Tyr Tyr Ala Asp Glu Lys Asp Val Asn Gly Asn Pro Pro Lys Glu
Leu 580 585 590 Leu Glu His Asp Lys Glu Leu Ala Pro Tyr Asn Tyr Gln
Val Ile Asp 595 600 605 Lys Lys 610 17659PRTCampylobacter jejuni
17Met Lys Ser Val Lys Leu Lys Val Ser Leu Ile Ala Asn Leu Ile Ala 1
5 10 15 Val Val Cys Leu Ile Ile Leu Gly Val Val Thr Phe Ile Phe Val
Lys 20 25 30 Gln Ala Ile Phe His Glu Val Val Asn Ala Glu Ile Asn
Tyr Val Lys 35 40 45 Thr Ala Lys Asn Ser Ile Glu Ser Phe Lys Ala
Arg Asn Ser Leu Ala 50 55 60 Leu Glu Ser Leu Ala Lys Ser Ile Leu
Lys His Pro Ile Glu Gln Leu 65 70 75 80 Asp Ser Gln Asp Ala Leu Met
His Tyr Val Gly Lys Asp Leu Lys Asn 85 90 95 Phe Arg Asp Ala Gly
Arg Phe Leu Ala Val Tyr Ile Ala Gln Pro Asn 100 105 110 Gly Glu Leu
Val Val Ser Asp Pro Asp Ser Asp Ala Lys Asn Leu Asp 115 120 125 Phe
Gly Thr Tyr Gly Lys Ala Asp Asn Tyr Asp Ala Arg Thr Arg Glu 130 135
140 Tyr Tyr Ile Glu Ala Val Lys Thr Asn Lys Leu Tyr Ile Thr Pro Ser
145 150 155 160 Tyr Ile Asp Val Thr Thr Asn Leu Pro Cys Phe Thr Tyr
Ser Ile Pro 165 170 175 Leu Tyr Lys Asp Gly Lys Phe Ile Gly Val Leu
Ala Val Asp Ile Leu 180 185 190 Ala Ala Asp Leu Gln Ala Glu Phe Glu
Asn Leu Pro Gly Arg Thr Phe 195 200 205 Val Phe Asp Glu Glu Asn Lys
Val Phe Val Ser Thr Asp Lys Ala Leu 210 215 220 Leu Gln Lys Gly Tyr
Asp Ile Ser Ala Ile Ala Asn Leu Ala Lys Thr 225 230 235 240 Lys Glu
Asp Leu Glu Pro Phe Glu Tyr Thr Arg Pro Lys Asp Gly Asn 245 250 255
Glu Arg Phe Ala Val Cys Thr Lys Val Ser Gly Ile Tyr Thr Ala Cys 260
265 270 Val Gly Glu Pro Ile Glu Gln Ile Glu Ala Pro Val Tyr Lys Ile
Ala 275 280 285 Phe Ile Gln Thr Ala Ile Val Ile Phe Thr Ser Ile Ile
Ser Val Ile 290 295 300 Leu Leu Tyr Phe Ile Val Ser Lys Tyr Leu Ser
Pro Leu Ala Ala Ile 305 310 315 320 Gln Thr Gly Leu Thr Ser Phe Phe
Asp Phe Ile Asn Tyr Lys Thr Lys 325 330 335 Asn Val Ser Thr Ile Glu
Val Lys Ser Asn Asp Glu Phe Gly Gln Ile 340 345 350 Ser Asn Ala Ile
Asn Glu Asn Ile Leu Ala Thr Lys Arg Gly Leu Glu 355 360 365 Gln Asp
Asn Gln Ala Val Lys Glu Ser Val Gln Thr Val Ser Val Val 370 375 380
Glu Gly Gly Asn Leu Thr Ala Arg Ile Thr Ala Asn Pro Arg Asn Pro 385
390 395 400 Gln Leu Ile Glu Leu Lys Asn Val Leu Asn Lys Leu Leu Asp
Val Leu 405 410 415 Gln Ala Arg Val Gly Ser Asp Met Asn Ala Ile His
Lys Ile Phe Glu 420 425 430 Glu Tyr Lys Ser Leu Asp Phe Arg Asn Lys
Leu Glu Asn Ala Ser Gly 435 440 445 Ser Val Glu Leu Thr Thr Asn Ala
Leu Gly Asp Glu Ile Val Lys Met 450 455 460 Leu Lys Gln Ser Ser Asp
Phe Ala Asn Ala Leu Ala Asn Glu Ser Gly 465 470 475 480 Lys Leu Gln
Thr Ala Val Gln Ser Leu Thr Thr Ser Ser Asn Ser Gln 485 490 495 Ala
Gln Ser Leu Glu Glu Thr Ala Ala Ala Leu Glu Glu Ile Thr Ser 500 505
510 Ser Met Gln Asn Val Ser Val Lys Thr Ser Asp Val Ile Thr Gln Ser
515 520 525 Glu Glu Ile Lys Asn Val Thr Gly Ile Ile Gly Asp Ile Ala
Asp Gln 530 535 540 Ile Asn Leu Leu Ala Leu Asn Ala Ala Ile Glu Ala
Ala Arg Ala Gly 545 550 555 560 Glu His Gly Arg Gly Phe Ala Val Val
Ala Asp Glu Val Arg Lys Leu 565 570 575 Ala Glu Arg Thr Gln Lys Ser
Leu Ser Glu Ile Glu Ala Asn Thr Asn 580 585 590 Leu Leu Val Gln Ser
Ile Asn Asp Met Ala Glu Ser Ile Lys Glu Gln 595 600 605 Thr Ala Gly
Ile Thr Gln Ile Asn Asp Ser Val Ala Gln Ile Asp Gln 610 615 620 Thr
Thr Lys Asp Asn Val Glu Ile Ala Asn Glu Ser Ala Ile Ile Ser 625 630
635 640 Ser Thr Val Ser Asp Ile Ala Asn Asn Ile Leu Glu Asp Val Lys
Lys 645 650 655 Lys Arg Phe 18665PRTCampylobacter jejuni 18Met Gln
Ser Ile Asn Ser Gly Lys Ser Val Gly Ile Ser Ala Lys Leu 1 5 10 15
Thr Leu Trp Val Gly Ile Leu Val Val Leu Ile Leu Ala Ile Thr Ser 20
25 30 Ala Ile Ser Tyr Phe Asp Ser Arg Asn Asn Thr Tyr Glu Leu Leu
Lys 35 40 45 Asp
Thr Gln Leu Lys Thr Met Gln Asp Val Asp Ala Phe Phe Lys Ser 50 55
60 Tyr Ala Met Ser Lys Arg Asn Gly Ile Gln Ile Leu Ala Asn Glu Leu
65 70 75 80 Thr Asn Arg Pro Asp Met Ser Asp Glu Glu Leu Ile Asn Leu
Ile Lys 85 90 95 Val Ile Lys Lys Val Asn Asp Tyr Asp Leu Val Tyr
Val Gly Phe Asp 100 105 110 Asn Thr Gly Lys Asn Tyr Gln Ser Asp Asp
Gln Ile Leu Asp Leu Ser 115 120 125 Lys Gly Tyr Asp Thr Lys Asn Arg
Pro Trp Tyr Lys Ala Ala Lys Glu 130 135 140 Ala Lys Lys Leu Ile Val
Thr Glu Pro Tyr Lys Ser Ala Ala Ser Gly 145 150 155 160 Glu Val Gly
Leu Thr Tyr Ala Ala Pro Phe Tyr Asp Arg Asn Gly Asn 165 170 175 Phe
Arg Gly Val Val Gly Gly Asp Tyr Asp Leu Ala Asn Phe Ser Thr 180 185
190 Asn Val Leu Thr Val Gly Lys Ser Asp Asn Thr Phe Thr Glu Val Leu
195 200 205 Asp Ser Glu Gly Thr Ile Leu Phe Asn Asp Glu Val Ala Lys
Ile Leu 210 215 220 Thr Lys Thr Glu Leu Ser Ile Asn Ile Ala Asn Ala
Ile Lys Ala Asn 225 230 235 240 Pro Ala Leu Ile Asp Pro Arg Asn Gln
Asp Thr Leu Phe Thr Ala Lys 245 250 255 Asp His Gln Gly Val Asp Tyr
Ala Ile Met Cys Asn Ser Ala Phe Asn 260 265 270 Pro Leu Phe Arg Ile
Cys Thr Ile Thr Glu Asn Lys Val Tyr Thr Glu 275 280 285 Ala Val Asn
Ser Ile Leu Met Lys Gln Val Ile Val Gly Ile Ile Ala 290 295 300 Ile
Ile Ile Ala Leu Ile Leu Ile Arg Phe Leu Ile Ser Arg Ser Leu 305 310
315 320 Ser Pro Leu Ala Ala Ile Gln Thr Gly Leu Thr Ser Phe Phe Asp
Phe 325 330 335 Ile Asn Tyr Lys Thr Lys Asn Val Ser Thr Ile Glu Val
Lys Ser Asn 340 345 350 Asp Glu Phe Gly Gln Ile Ser Asn Ala Ile Asn
Glu Asn Ile Leu Ala 355 360 365 Thr Lys Arg Gly Leu Glu Gln Asp Asn
Gln Ala Val Lys Glu Ser Val 370 375 380 Gln Thr Val Ser Val Val Glu
Gly Gly Asn Leu Thr Ala Arg Ile Thr 385 390 395 400 Ala Asn Pro Arg
Asn Pro Gln Leu Ile Glu Leu Lys Asn Val Leu Asn 405 410 415 Lys Leu
Leu Asp Val Leu Gln Ala Arg Val Gly Ser Asp Met Asn Ala 420 425 430
Ile His Lys Ile Phe Glu Glu Tyr Lys Ser Leu Asp Phe Arg Asn Lys 435
440 445 Leu Glu Asn Ala Ser Gly Ser Val Glu Leu Thr Thr Asn Ala Leu
Gly 450 455 460 Asp Glu Ile Val Lys Met Leu Lys Gln Ser Ser Asp Phe
Ala Asn Ala 465 470 475 480 Leu Ala Asn Glu Ser Gly Lys Leu Gln Thr
Ala Val Gln Ser Leu Thr 485 490 495 Thr Ser Ser Asn Ser Gln Ala Gln
Ser Leu Glu Glu Thr Ala Ala Ala 500 505 510 Leu Glu Glu Ile Thr Ser
Ser Met Gln Asn Val Ser Val Lys Thr Ser 515 520 525 Asp Val Ile Thr
Gln Ser Glu Glu Ile Lys Asn Val Thr Gly Ile Ile 530 535 540 Gly Asp
Ile Ala Asp Gln Ile Asn Leu Leu Ala Leu Asn Ala Ala Ile 545 550 555
560 Glu Ala Ala Arg Ala Gly Glu His Gly Arg Gly Phe Ala Val Val Ala
565 570 575 Asp Glu Val Arg Lys Leu Ala Glu Arg Thr Gln Lys Ser Leu
Ser Glu 580 585 590 Ile Glu Ala Asn Thr Asn Leu Leu Val Gln Ser Ile
Asn Asp Met Ala 595 600 605 Glu Ser Ile Lys Glu Gln Thr Ala Gly Ile
Thr Gln Ile Asn Asp Ser 610 615 620 Val Ala Gln Ile Asp Gln Thr Thr
Lys Asp Asn Val Glu Ile Ala Asn 625 630 635 640 Glu Ser Ala Ile Ile
Ser Ser Thr Val Ser Asp Ile Ala Asn Asn Ile 645 650 655 Leu Glu Asp
Val Lys Lys Lys Arg Phe 660 665 19750PRTCampylobacter jejuni 19Met
Arg Ile Thr Asn Lys Leu Asn Phe Thr Asn Ser Val Asn Asn Ser 1 5 10
15 Met Gly Gly Gln Ser Ala Leu Tyr Gln Ile Ser Gln Gln Leu Ala Ser
20 25 30 Gly Leu Lys Ile Gln Asn Ser Tyr Glu Asp Ala Ser Thr Tyr
Ile Asp 35 40 45 Asn Thr Arg Leu Glu Tyr Glu Ile Lys Thr Leu Glu
Gln Val Lys Glu 50 55 60 Ser Thr Ser Arg Ala Gln Glu Met Thr Gln
Asn Ser Met Lys Ala Leu 65 70 75 80 Gln Asp Met Val Lys Leu Leu Glu
Asp Phe Lys Val Lys Val Thr Gln 85 90 95 Ala Ala Ser Asp Ser Asn
Ser Gln Thr Ser Arg Glu Ala Ile Ala Lys 100 105 110 Glu Leu Glu Arg
Ile Lys Glu Ser Ile Val Gln Leu Ala Asn Thr Ser 115 120 125 Val Asn
Gly Gln Tyr Leu Phe Ala Gly Ser Gln Val Ala Asn Lys Pro 130 135 140
Phe Asp Ser Asn Gly Asn Tyr Tyr Gly Asp Lys Asn Asn Ile Asn Val 145
150 155 160 Val Thr Gly Ala Gly Thr Glu Ser Pro Tyr Asn Ile Pro Gly
Trp Asp 165 170 175 Leu Phe Phe Lys Ala Asp Gly Asp Tyr Lys Lys Gln
Ile Ser Thr Asn 180 185 190 Val Ser Phe Thr Asp Asn Arg Trp Asp Leu
Asn Lys Asp Pro Asp Lys 195 200 205 Thr Lys Tyr Leu Thr Gly Asp Ser
Lys Trp Gln Gln Leu Ile Gly Gln 210 215 220 Ser Tyr Val Lys Asp Asn
Ser Leu Asp Ala Asp Lys Asp Phe Glu Tyr 225 230 235 240 Asp Asp Ser
Lys Leu Asp Phe Pro Pro Thr Thr Leu Tyr Val Gln Gly 245 250 255 Thr
Arg Pro Asp Gly Thr Ser Phe Lys Ser Ala Val Leu Val Lys Pro 260 265
270 Glu Asp Thr Leu Glu Asp Val Met Glu Asn Ile Gly Ala Leu Tyr Gly
275 280 285 Asn Thr Pro Asn Asn Lys Val Val Glu Val Ser Met Asn Asp
Ser Gly 290 295 300 Gln Ile Gln Ile Thr Asp Leu Lys Gln Gly Asn Asn
Lys Leu Asp Phe 305 310 315 320 His Ala Val Ala Phe Thr Pro Gln Ala
Asp Asp Lys Thr Glu Leu Asn 325 330 335 Asn Ile Ile Gln Ala Ala Gln
Asp Glu Gly Ile Thr Met Glu Asp Val 340 345 350 Thr Asn Arg Val Met
Thr Ala Ala Leu Gly Asn Pro Asn Asn Gly Asp 355 360 365 Ile Thr Asn
Leu Asn Asn Pro Val Thr Ile Gln Ile Asn Gly Gln Asn 370 375 380 Phe
Glu Ile Asp Leu Lys Gln Thr Asp Phe Ile Lys Ser Lys Met Thr 385 390
395 400 Asp Thr Asp Gly Asn Ala Ala Asn Gly Ala Asp Tyr Asp Asn Val
Tyr 405 410 415 Phe Glu Lys Asn Gly Asn Thr Val Tyr Gly Asn Val Ser
Gln Val Ile 420 425 430 Lys Gly Ser Asn Ala Tyr Ala Thr Asp Ser Thr
Lys Leu Ser Glu Val 435 440 445 Met Ala Gly Asp Ser Leu Asn Gly Thr
Thr Leu Asn Leu Lys Val Asn 450 455 460 Ser Lys Gly Gly Asn Ser Tyr
Asp Val Thr Ile Asn Leu Gln Thr Ser 465 470 475 480 Thr Val Ser Tyr
Pro Asp Pro Asn Asn Pro Gly Gln Thr Ile Ser Phe 485 490 495 Pro Ile
Met His Thr Asn Pro Ala Thr Gly Asn Ser Gly Val Val Thr 500 505 510
Gly Ser Asn Asp Ile Thr Tyr Gly Gln Ile Asn Asp Ile Ile Gly Met 515
520 525 Phe Ala Ala Asp Lys Ile Pro Thr Thr Thr Ile Gln Ala Asn Asn
Gly 530 535 540 Gln Ile Asn Asn Ala Asp Tyr Thr Gln Ile Gln Gln Leu
Met Lys Asp 545 550 555 560 Ser Gln Ala Thr Val Asp Val Ser Met Asp
Tyr Lys Gly Arg Ile Ser 565 570 575 Val Thr Asp Lys Leu Ser Ser Gly
Thr Asn Ile Glu Ile Ser Leu Ser 580 585 590 Asp Ser Gln Ser Gly Gln
Phe Pro Ala Pro Pro Phe Thr Thr Thr Ser 595 600 605 Thr Val Gln Asn
Gly Pro Asn Phe Ser Phe Ser Ala Asn Asn Ser Leu 610 615 620 Thr Ile
Asp Glu Pro Asn Val Asp Ile Ile Lys Asp Leu Asp Ser Met 625 630 635
640 Ile Asp Ala Val Leu Lys Gly Asn Met Arg Ala Asp Ser Glu Ser Glu
645 650 655 Asn Pro Arg Asn Thr Gly Met Gln Gly Ala Leu Glu Arg Leu
Asp His 660 665 670 Leu Ala Asp His Val Ser Lys Leu Asn Thr Thr Met
Gly Ala Tyr His 675 680 685 Asn Thr Ile Glu Gly Val Asn Thr Arg Thr
Ser Phe Leu Ser Val Asn 690 695 700 Val Gln Ser Ile Lys Ser Asn Val
Ile Asp Val Asp Tyr Gly Glu Ala 705 710 715 720 Met Met Asn Leu Met
Gln Val Gln Leu Ala Tyr Gln Ala Ser Leu Lys 725 730 735 Ala Ser Thr
Thr Ile Ser Gln Leu Ser Leu Leu Asn Tyr Met 740 745 750
20865PRTCampylobacter jejuni 20Met Met Arg Ser Leu Trp Ser Gly Val
Ser Gly Leu Gln Ala His Gln 1 5 10 15 Val Ala Met Asp Val Glu Gly
Asn Asn Ile Ser Asn Val Asn Thr Thr 20 25 30 Gly Phe Lys Tyr Ser
Arg Ala Asp Phe Gly Thr Met Phe Ser Gln Thr 35 40 45 Val Lys Ile
Ala Thr Ala Pro Thr Asp Gly Arg Gly Gly Ser Asn Pro 50 55 60 Leu
Gln Ile Gly Leu Gly Val Ser Val Ser Ser Thr Thr Arg Ile His 65 70
75 80 Ser Gln Gly Ser Val Gln Thr Thr Asp Lys Asn Thr Asp Val Ala
Ile 85 90 95 Asn Gly Asp Gly Phe Phe Met Val Ser Asp Asp Gly Gly
Leu Thr Asn 100 105 110 Tyr Leu Thr Arg Ser Gly Asp Phe Lys Leu Asp
Ala Tyr Gly Asn Phe 115 120 125 Val Asn Asn Ala Gly Phe Val Val Gln
Gly Trp Asn Ile Asn Trp Asp 130 135 140 Asp Gln Thr Ile Asp Ser Ser
Arg Thr Pro Gln Asn Ile Phe Ile Asp 145 150 155 160 Pro Gly Met His
Ile Pro Ala Ala Lys Ser Thr Glu Val Ala Ile Lys 165 170 175 Ala Asn
Leu Asn Ser Gly Leu Asn Ile Gly Thr Ser Ser Arg Asn Leu 180 185 190
Tyr Ala Leu Asp Ser Val His Gly Trp Asn Thr Lys Thr Gln Arg Ala 195
200 205 Glu Asp Glu Asn Asp Thr Gly Thr Thr Gln Phe Tyr Thr Thr Ser
Lys 210 215 220 Asn Ser Val Glu Val Thr Glu Lys Gly Val Asp Ala Gly
Ser Leu Phe 225 230 235 240 Asn Ala Lys Gly Gln Gly Leu Asn Leu Arg
Asp Gly Gln Gly Ile Trp 245 250 255 Val Ser Tyr Ala Asp Ala Thr Tyr
Ser Thr Asn Lys Val Gly Val Asn 260 265 270 Ala Phe Asp Pro Asn Leu
Gln Gln Asn Gln Thr Ala Ala Phe Trp Gly 275 280 285 Thr Ala Asn Gln
Lys Val Asn Leu Asp Ile Thr Leu Asn Gly Val Arg 290 295 300 Ile Gln
Asn Ala Asp Ile Gln Ser Ile Asp Asp Ala Ile Ala Tyr Ile 305 310 315
320 Asn Thr Phe Thr Ala Pro Thr Asp Thr Arg Asp Gly Thr Gly Val Lys
325 330 335 Ala Val Lys Asn Lys Asp Gly Ser Gly Ile Asp Phe Val Asn
Asp Asn 340 345 350 Ala Asp Gly Thr Thr Asp Asn Met Lys Asn Ile Asn
Leu Val Val Ala 355 360 365 Asn Thr Asn Thr Ala Gly Glu Leu Trp Asn
Ala Val Trp Asn Asn Asn 370 375 380 Asn Gln Thr Phe Thr Phe Asn Asn
Asn Gly Asn Gly Gln Ala Gly Thr 385 390 395 400 Pro Thr Ile Asn Lys
Asn Gly Ser Ser Leu Trp Thr Ala Thr Asn Ile 405 410 415 Thr Phe Thr
Pro Gln Pro Pro Gln Ala Ala Thr Asn Val Gln Leu Thr 420 425 430 Gly
Gly Leu Asn Ala Gln Ile Ile Thr Ala His Lys Tyr Ile Tyr Ser 435 440
445 Ser Asn Pro Val Asp Ile Gly Pro Met Tyr Asn Pro Asp Gly Gly Pro
450 455 460 Ala Phe Gln Pro Gly Ala Asn Ala Thr Thr Arg Pro Thr Glu
Pro Gly 465 470 475 480 Ser Ala Ala Tyr Trp Asp Ala Val Asn Gly Gly
Leu Leu Asn Thr Asn 485 490 495 Val Arg Thr Phe Arg Thr Thr Glu Asp
Leu Arg Glu Leu Leu Gln Arg 500 505 510 Asp Ala Arg Tyr Gly Val Asp
Tyr Asp Gly Ser Gly Thr Phe Ala Ala 515 520 525 Ala Asp Ile Asn Gln
Asn Ile Lys Val Val Val Thr Ala Asp Gly His 530 535 540 Phe Ala Ile
Ser Asn Ala Asn Glu Gln Ser Thr Val Pro Pro Asn Ala 545 550 555 560
Ile Asn Gly Val Gly Asn Ala Thr Thr Thr Asp Pro Lys Asn Met Ser 565
570 575 Phe Asn Ile Thr Ala Tyr Ser Asn Lys Gln Gly Thr Val Ser Thr
Asn 580 585 590 Asp Ala Phe Thr Ala Ile Phe Lys Ala Phe Asp Gly Pro
Leu Val Ile 595 600 605 Gly Asn Gln Ile Lys Glu Ser Glu Gln Leu Lys
Leu Ser Ala Phe Ser 610 615 620 Ala Gly Leu Glu Ile Tyr Asp Ser Leu
Gly Ser Lys His Thr Leu Glu 625 630 635 640 Val Gln Phe Val Lys Gln
Ser Thr Thr Gln Asp Gly Gly Asn Glu Trp 645 650 655 Gln Met Ile Ile
Arg Val Pro Glu Pro Ala Glu Ile Asn Thr Thr Gly 660 665 670 Glu Gly
Pro Asn Asn Ile Ile Val Gly Thr Ala Arg Phe Asn Asn Asp 675 680 685
Gly Ser Leu Ala Ser Tyr Thr Pro Arg Thr Ile Asn Phe Ser Pro Asn 690
695 700 Asn Gly Ala Ala Pro Asn Gln Gln Ile Lys Leu Ser Phe Gly Thr
Ser 705 710 715 720 Gly Ser Asn Asp Gly Leu Val Ser Ser Asn Ser Ala
Ser Thr Leu Thr 725 730 735 Gly Gln Ala Thr Asp Gly Tyr Thr Ser Gly
Asn Leu Lys Pro Asp Ala 740 745 750 Ile Arg Val Asp Asp Lys Gly Asn
Ile Leu Gly Glu Phe Thr Asn Gly 755 760 765 Lys Thr Phe Ala Val Ala
Lys Ile Ala Met Ala Ser Val Ala Asn Asn 770 775 780 Ser Gly Leu Glu
Glu Ile Gly Gly Asn Leu Phe Lys Val Thr Ala Asn 785 790 795 800 Ser
Gly Asn Ile Val Val Gly Glu Ala Gly Thr Gly Gly Arg Gly Glu 805 810
815 Met Lys Thr Ser Ala Leu Glu Met Ser Asn Val Asp Leu Ser Arg Ser
820 825 830 Leu Thr Glu Leu Ile Ile Ile Gln Arg Gly Tyr Gln Ala Asn
Ser Lys 835 840 845 Thr Ile Ser Thr Ser Asp Gln Met Leu Gln Thr Leu
Ile Gln Leu Lys 850 855 860 Gln 865 21871PRTCampylobacter jejuni
21Met Ala Lys Ile Arg Ile His Glu Ile Ala Lys Glu Leu Gly Tyr Asp 1
5 10 15 Ser Lys Glu Ile Ile Glu Lys Ala Asn Glu Leu Gly Leu Gly Ile
Lys 20 25 30 Thr Ala Ser Asn Ala Val Glu Pro Glu Ile Ala Ala Ala
Ile Tyr Glu 35 40 45 Tyr Ile Gln Thr Arg Glu Ile Pro Glu Ala Phe
Lys Lys Asn Ile Lys 50 55 60
Thr Pro Thr Ala Lys Lys Pro Lys Lys Glu Asn Ile Lys Glu Gln Glu 65
70 75 80 Lys Leu Asn Glu Ser Glu Lys Lys Glu Pro Lys Lys Glu Glu
Lys Leu 85 90 95 Lys Gln Glu Val Lys Lys Glu Glu Leu Lys Ile Glu
Lys Glu Asn Ala 100 105 110 Lys Glu Glu Glu Lys Gln Glu Ile Ile Asp
Ala His Lys Pro Gln Ser 115 120 125 Leu Ala Ser Ala Thr Leu Ala Lys
Arg Arg Gly Leu Val Ile Val Lys 130 135 140 Lys Lys Lys Asp Glu Glu
Glu Ile Gln Val Lys Lys Glu Glu Val Lys 145 150 155 160 Asn Ser Asn
Asp Ile Ser Ile Asn Asn Glu Glu Arg Leu Ser Leu Lys 165 170 175 Thr
Met Phe Ser Asn Ala Asp Glu Ser Leu Lys Lys Lys Lys Lys Glu 180 185
190 Lys Lys Ser Phe Val Ala Ser Lys Lys Glu Ser Thr Glu Lys Met Asn
195 200 205 Phe Leu Asp Glu His Asp Phe Gly Asp Ile Ser Leu Asp Asp
Glu Asp 210 215 220 Glu Val Val Leu Pro Asp Phe Ser Val Lys Glu Gln
Glu Lys Pro Gln 225 230 235 240 Asn Ile Asn Lys Lys Gln Pro Asn Phe
Ile Arg Gln Ala Val Gly Asn 245 250 255 Ser Ala Gly Phe Gly Phe Glu
Gly Gly Ile Gln Arg Arg Ser Arg Lys 260 265 270 Lys Pro Ser Lys Lys
Ile Glu Lys Lys Glu Val Glu Glu Val Gly Ser 275 280 285 Val Ala Ile
Ser Lys Glu Ile Arg Val Tyr Glu Phe Ala Asp Lys Ile 290 295 300 Gly
Lys Ser Thr Ser Glu Val Ile Ser Lys Leu Phe Met Leu Gly Met 305 310
315 320 Met Thr Thr Lys Asn Asp Phe Leu Asp Glu Asp Ala Ile Glu Ile
Leu 325 330 335 Ala Ala Glu Phe Gly Ile Glu Ile Asn Ile Ile Asn Glu
Ala Asp Glu 340 345 350 Phe Asp Tyr Val Lys Asp Tyr Glu Glu Glu Thr
Asp Glu Lys Asp Leu 355 360 365 Val Thr Arg Ala Pro Val Ile Thr Ile
Met Gly His Val Asp His Gly 370 375 380 Lys Thr Ser Leu Leu Asp Tyr
Ile Arg Lys Ser Arg Val Ala Ser Gly 385 390 395 400 Glu Ala Gly Gly
Ile Thr Gln His Val Gly Ala Tyr Met Val Glu Lys 405 410 415 Asn Gly
Arg Lys Ile Thr Phe Ile Asp Thr Pro Gly His Glu Ala Phe 420 425 430
Thr Ala Met Arg Ala Arg Gly Ala Ser Ile Thr Asp Ile Val Ile Ile 435
440 445 Val Val Ala Ala Asp Asp Gly Val Lys Pro Gln Thr Lys Glu Ala
Ile 450 455 460 Asn His Ala Lys Ala Ala Gly Val Pro Ile Ile Ile Ala
Ile Asn Lys 465 470 475 480 Met Asp Lys Glu Ala Ala Asn Pro Asp Met
Val Lys Thr Gln Leu Ala 485 490 495 Glu Met Glu Ile Met Pro Val Glu
Trp Gly Gly Ser Tyr Glu Phe Val 500 505 510 Gly Val Ser Ala Lys Thr
Gly Met Gly Ile Glu Asp Leu Leu Glu Ile 515 520 525 Val Leu Leu Gln
Ala Asp Ile Leu Glu Leu Lys Ala Asn Pro Lys Ser 530 535 540 Phe Ala
Lys Ala Ser Ile Ile Glu Ser Ser Val Gln Lys Gly Arg Gly 545 550 555
560 Ala Val Ala Thr Val Ile Val Gln Asn Gly Thr Leu Thr Val Gly Ser
565 570 575 Thr Val Val Ala Gly Glu Ala Tyr Gly Lys Val Arg Ala Met
Ser Asp 580 585 590 Asp Gln Gly Lys Ala Leu Lys Glu Ile Lys Pro Gly
Glu Cys Gly Val 595 600 605 Ile Val Gly Leu Ser Glu Val Ala Asp Ala
Gly Glu Ile Leu Ile Ala 610 615 620 Val Lys Thr Asp Lys Glu Ala Arg
Glu Tyr Ala Asn Lys Arg His Glu 625 630 635 640 Tyr Asn Arg Gln Lys
Glu Leu Ser Lys Ser Thr Lys Val Ser Ile Asp 645 650 655 Glu Leu Gly
Ala Lys Ile Lys Glu Gly Asn Leu Lys Ala Leu Pro Val 660 665 670 Ile
Leu Lys Ala Asp Val Gln Gly Ser Leu Glu Ala Leu Lys Ala Ser 675 680
685 Leu Glu Lys Leu Arg Asn Asp Glu Ile Lys Val Asn Ile Ile His Ser
690 695 700 Gly Val Gly Gly Ile Thr Gln Ser Asp Ile Glu Leu Ala Ser
Ala Ser 705 710 715 720 Glu Asn Ser Ile Val Leu Gly Phe Asn Ile Arg
Pro Thr Gly Glu Val 725 730 735 Lys Glu Arg Ala Lys Asp Lys Gly Val
Glu Ile Lys Thr Tyr Asn Val 740 745 750 Ile Tyr Asn Leu Leu Asp Asp
Val Lys Ala Leu Leu Gly Gly Met Met 755 760 765 Ser Pro Ile Ile Ser
Glu Glu Gln Leu Gly Gln Ala Glu Ile Arg Gln 770 775 780 Val Ile Asn
Val Pro Lys Ile Gly Gln Ile Ala Gly Cys Met Val Thr 785 790 795 800
Glu Gly Val Ile Asn Arg Gly Ala Lys Ile Arg Leu Ile Arg Asp Gly 805
810 815 Val Val Val Tyr Glu Gly Asn Val Ser Ser Leu Lys Arg Phe Lys
Asp 820 825 830 Asp Ala Lys Glu Val Ala Lys Gly Tyr Glu Cys Gly Val
Gly Ile Glu 835 840 845 Gly Cys Asp Asp Met Arg Val Gly Asp Tyr Ile
Glu Ser Tyr Lys Glu 850 855 860 Val Glu Glu Gln Ala Ser Leu 865 870
22946PRTCampylobacter jejuni 22Met Leu Ala Pro Gly Met Gly Glu Trp
Val Tyr Lys Ala Asn Leu Phe 1 5 10 15 Leu Phe Gly Glu Phe Ala Tyr
Tyr Tyr Pro Phe Phe Leu Phe Ile Leu 20 25 30 Asn Tyr Val Tyr Tyr
Lys Arg Asn Tyr Lys Leu Ala Asn Phe Thr Arg 35 40 45 Arg Glu Leu
Phe Gly Ile Gly Phe Ala Phe Phe Ser Ser Leu Leu Leu 50 55 60 Phe
Ala Val Phe Tyr Pro Asn Ser Gly Tyr Ile Leu Glu Leu Ala Tyr 65 70
75 80 Ala Ile Phe Ser Thr Ile Leu Gly His Thr Gly Ser Gly Ile Phe
Ala 85 90 95 Leu Leu Leu Leu Leu Phe Ser Leu Val Leu Leu Phe Pro
Lys Phe Ala 100 105 110 Lys Glu Ile Leu Lys Ile Glu Leu Asp Phe Thr
Tyr Leu Leu Lys Val 115 120 125 Glu Gln Ala Phe Lys Ser Leu Leu Met
Arg Val Phe Gly Gly Glu Asn 130 135 140 Glu Lys Glu Asp Val Gly Lys
Ser Glu Pro Ile Val Pro Lys Leu Asn 145 150 155 160 Ile Leu Gln Asp
Ser Ile Tyr Gly Asn Leu Gln Ile Asn Lys Lys Gly 165 170 175 Glu Thr
Asn Asn Leu Glu Gln Ile Ile Lys Asp Ser Asn Ile Asn Ala 180 185 190
Ser Lys Asn Ser Ile Thr Thr Ala Lys Glu Asn Phe Glu Lys Leu Lys 195
200 205 Asn Gln Ile Leu Asp Glu Thr Ile Glu Ile Asp Lys Gln Ser Leu
Lys 210 215 220 Glu Ser Arg Ser Phe Val His Glu His Ser Gln Gln Val
Arg Asn Phe 225 230 235 240 Ala Gln Lys Ala Ser Lys Met Ser Ile Ser
Leu Asp Glu Asp Phe Asn 245 250 255 Phe Ile Ser Glu Glu Glu Val Asp
Met Ile Pro Glu Arg Phe Leu Lys 260 265 270 Pro Lys Lys Leu Glu Asp
Ile Lys Gln Ile Asp Thr Asn Lys Asn Leu 275 280 285 Asp Glu Pro Ser
Tyr Lys Arg Lys Asn Ile Glu Ile Pro Val Ser Asn 290 295 300 Gln Glu
Val Lys Pro Lys Ile Phe Thr Lys Glu Leu Glu Leu Arg Glu 305 310 315
320 Asn Leu Ile Lys Lys Glu Lys Leu Glu Gln Glu Tyr Lys Ala Tyr Gln
325 330 335 Asn Glu Ile Leu Glu Asn Lys Val Lys Gln Glu Ile Lys Lys
Leu Glu 340 345 350 Glu Tyr Asp Ala Ile Asn Ser Ser Asp Ile Ile Glu
Gly Asn Lys Tyr 355 360 365 Ser Phe Asn Ser Pro Lys Thr Ile Lys Thr
Glu Thr Glu Glu Ser Asp 370 375 380 Lys Ile Asn Glu Asn Lys Asn Leu
Asp Lys Ala Asp Asn Ile Phe Glu 385 390 395 400 Phe Ala Pro Ile Val
Glu Glu Leu Asn His Pro Tyr Ile Glu Pro Thr 405 410 415 Pro Ile Lys
Asn Ile Asn Glu Ile Val Ile Glu Glu Lys Asn Thr Leu 420 425 430 Asp
Phe Ile Gln Asn Thr Glu Thr Lys Ile Asp Asn Glu Lys Thr Asn 435 440
445 Asp Gln Glu Ile Lys Leu Gln Lys Ala Val Leu Ala Lys Glu Ile Ala
450 455 460 Ile Asn Gln Ala Leu Leu Arg Glu Ile Glu Gln Gly Glu Ile
Glu Lys 465 470 475 480 Pro Lys Asp Phe Thr Leu Pro Pro Leu Asp Phe
Leu Ala Asn Pro Lys 485 490 495 Glu His Lys Gln Glu Ile Asn Glu Ser
Glu Ile Asp Lys Lys Ile Tyr 500 505 510 Asn Leu Leu Glu Lys Leu Arg
Arg Phe Lys Ile Gly Gly Asp Val Ile 515 520 525 Ser Thr Tyr Val Gly
Pro Val Val Thr Thr Phe Glu Phe Arg Pro Ser 530 535 540 Ala Asp Val
Lys Val Ser Arg Ile Leu Asn Leu Gln Asp Asp Leu Thr 545 550 555 560
Met Ala Leu Met Ala Lys Ser Ile Arg Ile Gln Ala Pro Ile Pro Gly 565
570 575 Lys Asp Val Val Gly Ile Glu Val Pro Asn Asp Glu Ile Gln Thr
Ile 580 585 590 Tyr Leu Arg Glu Ile Leu Gln Ser Glu Val Phe Lys Asn
Ala Lys Ser 595 600 605 Pro Leu Thr Ile Ala Leu Gly Lys Asp Ile Val
Gly Asn Ala Phe Val 610 615 620 Thr Asp Leu Lys Lys Leu Pro His Leu
Leu Ile Ala Gly Thr Thr Gly 625 630 635 640 Ser Gly Lys Ser Val Gly
Ile Asn Ser Met Leu Leu Ser Leu Leu Tyr 645 650 655 Arg Asn Ser Pro
Lys Thr Leu Arg Leu Met Met Ile Asp Pro Lys Met 660 665 670 Leu Glu
Phe Ser Ile Tyr Asn Asp Ile Pro His Leu Leu Thr Pro Val 675 680 685
Ile Thr Asp Pro Lys Lys Ala Val Asn Ala Leu Ser Asn Met Val Ala 690
695 700 Glu Met Glu Arg Arg Tyr Arg Leu Met Ala Asp Ala Lys Thr Lys
Asn 705 710 715 720 Ile Glu Asn Tyr Asn Glu Lys Met Lys Glu Leu Gly
Gly Glu Lys Leu 725 730 735 Pro Phe Ile Val Val Ile Ile Asp Glu Leu
Ala Asp Leu Met Met Thr 740 745 750 Ala Gly Lys Asp Val Glu Phe Tyr
Ile Gly Arg Leu Ala Gln Met Ala 755 760 765 Arg Ala Ser Gly Ile His
Leu Ile Val Ala Thr Gln Arg Pro Ser Val 770 775 780 Asp Val Val Thr
Gly Leu Ile Lys Ala Asn Leu Pro Ser Arg Ile Ser 785 790 795 800 Tyr
Lys Val Gly Gln Lys Ile Asp Ser Lys Val Ile Leu Asp Ala Met 805 810
815 Gly Ala Glu Ser Leu Leu Gly Arg Gly Asp Cys Leu Phe Thr Pro Pro
820 825 830 Gly Thr Ser Ser Ile Val Arg Leu His Ala Pro Phe Ala Ser
Glu Phe 835 840 845 Glu Ile Glu Lys Ile Val Asp Phe Leu Lys Asp Gln
Gln Ser Val Glu 850 855 860 Tyr Asp Glu Ser Phe Leu Lys Asp Gln Gln
Ser Val Gly Val Thr Thr 865 870 875 880 Asn Glu Ser Phe Asp Gly Glu
Ala Asp Glu Leu Tyr Glu Glu Ala Lys 885 890 895 Arg Val Ile Leu Glu
Asp Gly Lys Thr Ser Ile Ser Tyr Leu Gln Arg 900 905 910 Arg Leu Lys
Ile Gly Tyr Asn Arg Ser Ala Asn Ile Ile Glu Gln Leu 915 920 925 Thr
Gln Asn Gly Ile Leu Ser Glu Pro Asp Ala Lys Gly Gln Arg Glu 930 935
940 Ile Leu 945 231041PRTCampylobacter jejuni 23Met Lys Lys Phe Phe
Cys Leu Thr Leu Val Cys Lys Leu Phe Ala Leu 1 5 10 15 Ser Glu Phe
Glu Leu His His Ile Asp Lys Val His Lys Leu Gly Tyr 20 25 30 Ser
Gly Asp Thr Ile Ile Ile Gly Val Ala Asp Asp Ala Phe Asn Gln 35 40
45 Asp His Ile Ser Leu Lys Asp Lys Ile Leu Lys Ser Thr Tyr Pro Thr
50 55 60 Asp Thr Ala Gly Lys Gln Leu Ile Pro Asp Leu Lys Lys Ser
Thr His 65 70 75 80 Gly Ser His Val Ala Gly Ile Ala Val Gly Ala Lys
Ile Gly Asp Ser 85 90 95 Lys Pro Tyr Gly Val Ala Tyr Gly Ala Lys
Phe Tyr Gly Ala Gly Val 100 105 110 Phe Pro Asn Gly Ser Tyr Thr Gln
Ile Pro Asp Ile Tyr Asn Phe Phe 115 120 125 Lys Asp Val Ser Ile Ile
Asn Asn Ser Trp Gly Ile Asn Phe Tyr Pro 130 135 140 Tyr Phe Asn Leu
Lys Ala Ser Asn Ser Gly Leu Val Asp Cys Thr Gln 145 150 155 160 Thr
Asn Gln Gly Thr Ser Tyr Asn Ile Cys Asn Thr Pro Leu Glu Tyr 165 170
175 Val Met Lys Ala Asp Lys Val Ala Asn Asp Met Met Arg Leu Ser Lys
180 185 190 Asp Lys Gly Val Leu Asn Val Phe Ala Ala Gly Asn Glu Gly
Ile Leu 195 200 205 Ser Pro Ala Leu His Ala Ile Leu Pro Ser Tyr Asp
Glu Ser Leu Arg 210 215 220 Ala Trp Leu Ala Val Gly Ala Leu Asp Ala
Asn Glu Ile Thr Leu Glu 225 230 235 240 Ser Asp Gly Thr Leu Ile Ile
Lys Ser Gln Gly Leu Ala Asp Phe Ser 245 250 255 Asn Gly Phe Lys Gly
Ala Thr Asn Phe Ser Leu Val Ala Ala Gly Val 260 265 270 Asn Ile Asn
Asn Val Asp Ser Ser Thr Asn Asp Lys Phe Thr Lys Lys 275 280 285 Ser
Gly Thr Ser Met Ala Ala Pro Met Val Ser Gly Thr Ala Ala Leu 290 295
300 Val Lys Gln Asn Phe Pro Phe Leu Asp Gly Lys Gln Ile Ala Asp Ile
305 310 315 320 Leu Leu Ser Thr Ala Asn Lys Asn Tyr Lys Ala Pro Lys
Phe Thr Val 325 330 335 Lys Gln Val Thr Asp Gly Thr Asn Gln Pro Lys
Phe Leu Ile Val Tyr 340 345 350 Ile Ser Gln Asp Pro Pro Gly Ile Glu
Asp Glu Ile Lys Arg Asp Leu 355 360 365 Lys Gln Leu Tyr Asn Gly Ile
Gln Val Gln Val Asn Gly Gln Trp Ile 370 375 380 Asp Tyr Ser Asp Tyr
Ile Trp Asp Asn Arg Asp Ser Ala Gln Ser Gln 385 390 395 400 Lys Leu
Asn Thr Ser Thr Ile Ser Ser Ile Asn Gly Val Val Arg Val 405 410 415
Glu Lys Glu Glu Leu Phe Gly Gln Gly Ile Leu Asp Ala Gln Lys Ala 420
425 430 Leu Lys Gly Leu Ser Ile Leu Asp Ala Asn Arg Leu Ser Asp Gln
Asp 435 440 445 Val Leu Lys Tyr Glu Gln Glu Pro Asn Thr Ala Tyr Tyr
Thr Ile Asn 450 455 460 Thr Ala Gly Tyr Asp Ala Glu Phe Ser Asn Asp
Ile Ser Gln Arg Lys 465 470 475 480 Trp Asp Glu Ser Thr His Leu Ser
Ser Ala Ile Asn Lys Pro Thr His 485 490 495 Leu Ala Asn Leu Asn Ile
Gly Leu Ser Lys Glu Gly Glu Gly Ile Leu 500 505 510 Ile Ile Ser Gly
Gln Asn Thr Tyr Glu Gly Ala Thr Leu Ile Lys Gln 515 520 525 Gly Glu
Leu Lys Leu Lys Gly Lys Val Lys Asn Asn Ala Tyr Val Glu 530 535 540
Gln Lys Ala Ile
Leu Ser Gly Asn Gly Ile Val Gly Gln Asn Leu Asn 545 550 555 560 Asn
Lys Gly Ile Val Arg Pro Gly Asn Glu Asp Leu Asn Asp Leu Thr 565 570
575 Val Gln Gly Thr Tyr Thr Gln Glu Gly Val Asp Ser Lys Leu Gln Leu
580 585 590 Asp Phe Gly Asn Tyr Lys Asn Ser Lys Leu Ile Ala Lys Thr
Tyr Asp 595 600 605 Ile Lys Ser Gly Asn Leu Glu Tyr Ile Pro Leu Pro
Lys Tyr Tyr Ile 610 615 620 Leu Asn Lys Pro Val Lys Ile Asn Leu Gly
Asp Leu Glu Lys Ser Leu 625 630 635 640 Ser Ser Phe Asn His Val Leu
Ile Gln Asn Thr Tyr Ala Leu Asn Phe 645 650 655 Asp Phe Val Leu Ser
Asp Asp Leu Val Ser Ile Asn Lys Thr Leu Ile 660 665 670 Lys Pro Asn
Leu Lys Pro Asn Ala Tyr Glu Ile Pro Asn Thr Ser Leu 675 680 685 Gly
Asn Ala Leu Arg Gln Leu Arg Ser Arg Ala Asp Leu Ser Gln Thr 690 695
700 Tyr Gln Glu Phe Phe Ala Ser Leu Asp Asn Gly Ile Asp Val Lys Thr
705 710 715 720 Lys Leu Asn Arg Ile Glu Gly Ser Gly Tyr Leu Ser Thr
Phe Ser Asn 725 730 735 His Asn Gln Ser Asn Leu Met Gln Asn Asn Met
Leu Phe Thr Leu His 740 745 750 Pro Leu Asn Ile Asn Asn Phe Ala Gln
Asn Asn Asn Ile Leu Leu Ala 755 760 765 Ser Thr Tyr Leu Pro Arg Ile
Phe Ser Asn Glu Glu Tyr Phe Trp His 770 775 780 Leu Thr Pro Ser Tyr
Lys Tyr Tyr Lys Asp Lys Asp Phe Ser Gly Gln 785 790 795 800 Lys Thr
Gly Ala Asn Ile Ser Leu Gly Glu Asn Phe Ser Ser Gly Phe 805 810 815
Leu Ala Tyr Ala Leu Ser Leu Ser Ser Ala Lys Phe Asn Phe Asn Asn 820
825 830 Gly Ser Asp Leu Lys Ser Tyr Asn Met Asp Leu Leu Leu Asn Tyr
Asn 835 840 845 His Asp Leu Asp Phe Ile Lys Ile Leu Ser Gly Leu Gly
Ile Gly Val 850 855 860 Gly Phe Asn Thr Leu Asn Arg Phe Val Val Glu
Gln Pro Ile Glu Gly 865 870 875 880 Lys Tyr Lys Thr Leu Gln Thr Ser
Ala Gln Leu Gly Val Thr Lys Asp 885 890 895 Ile Ile Leu Gly Gln Asp
Phe Ile Phe Asn Pro Leu Met Tyr Phe Thr 900 905 910 His Ser Phe Phe
Tyr Gln Glu Asp Phe Lys Glu Asn Lys Ser Pro Phe 915 920 925 Ala Lys
Asn Tyr Glu Ser Leu Lys His His Ser Ile Asn Ala Asn Leu 930 935 940
Gly Phe Asn Leu Ala Lys Asn Ile Glu Gln Asp Asp Tyr Gln Ala Ser 945
950 955 960 Phe Ser Thr Phe Val Ile Phe Glu Lys Arg Ile Tyr Gly Arg
Thr Leu 965 970 975 Glu Asn Lys Ala Ser Phe Val Asp Phe Pro Ile Ala
Phe Ile Gln Lys 980 985 990 Tyr Lys Leu Lys Asp Asn Ile Leu Ser Gln
Gly Phe Asn Ser Glu Phe 995 1000 1005 Leu Tyr Lys Asn Asn Val Phe
Trp Gln Phe Met Leu Met Asn Arg 1010 1015 1020 Phe Ser His Asn Ala
Tyr Glu Leu His Leu Met Ser Ser Val Gly 1025 1030 1035 Lys Arg Phe
1040 241089PRTCampylobacter jejuni 24Met Pro Lys Arg Thr Asp Ile
Lys Ser Ile Leu Leu Ile Gly Ser Gly 1 5 10 15 Pro Ile Val Ile Gly
Gln Ala Cys Glu Phe Asp Tyr Ser Gly Thr Gln 20 25 30 Ala Ala Lys
Thr Leu Lys Glu Leu Gly Tyr Arg Val Val Leu Ile Asn 35 40 45 Ser
Asn Pro Ala Thr Ile Met Thr Asp Pro Glu Phe Ala Asp Ala Thr 50 55
60 Tyr Ile Glu Pro Ile Thr Lys Glu Ser Ile Leu Ser Ile Ile Lys Lys
65 70 75 80 Glu Lys Ile Asp Ala Ile Leu Pro Thr Met Gly Gly Gln Val
Ala Leu 85 90 95 Asn Val Ala Met Glu Val Tyr Glu Ser Gly Leu Leu
Gly Asp Val Lys 100 105 110 Phe Leu Gly Ala Asn Pro Glu Ala Ile Lys
Lys Gly Glu Asp Arg Gln 115 120 125 Val Phe Lys Glu Cys Met Lys Lys
Ile Gly Met Asp Leu Pro Lys Ser 130 135 140 Met Tyr Ala Tyr Asn Tyr
Asp Glu Ala Leu Lys Ala Val Asp Glu Ile 145 150 155 160 Asp Phe Pro
Leu Met Ile Arg Ala Ser Tyr Thr Leu Gly Gly Ala Gly 165 170 175 Ser
Gly Val Val Tyr Asn Met Asp Glu Phe Lys Glu Leu Thr Asn Thr 180 185
190 Ala Leu Ala Leu Ser Pro Ile His Glu Ile Leu Ile Glu Glu Ser Leu
195 200 205 Leu Gly Trp Lys Glu Tyr Glu Met Glu Val Ile Arg Asp Arg
Ala Asp 210 215 220 Asn Cys Ile Ile Val Cys Ser Ile Glu Asn Ile Asp
Pro Met Gly Val 225 230 235 240 His Thr Gly Asp Ser Ile Thr Ile Ala
Pro Ala Leu Thr Leu Thr Asp 245 250 255 Lys Glu Tyr Gln Val Met Arg
Asn Ala Ser Phe Ala Ile Leu Arg Glu 260 265 270 Ile Gly Val Asp Thr
Gly Gly Ser Asn Val Gln Phe Ala Ile Asn Pro 275 280 285 Lys Asn Gly
Arg Met Ile Val Ile Glu Met Asn Pro Arg Val Ser Arg 290 295 300 Ser
Ser Ala Leu Ala Ser Lys Ala Thr Gly Tyr Pro Ile Ala Lys Val 305 310
315 320 Ala Thr Leu Leu Ala Val Gly Phe Ser Leu Asp Glu Ile Lys Asn
Asp 325 330 335 Ile Thr Gly Thr Pro Ala Ser Phe Glu Pro Val Ile Asp
Tyr Ile Val 340 345 350 Thr Lys Ile Pro Arg Phe Thr Phe Glu Lys Phe
Pro Gly Ala Asn Thr 355 360 365 Thr Leu Gly Thr Ala Met Lys Ser Val
Gly Glu Val Met Ala Ile Gly 370 375 380 Arg Thr Phe Lys Glu Ser Ile
Gln Lys Ala Leu Cys Ser Leu Glu Arg 385 390 395 400 Ser Leu Ser Gly
Phe Asp Arg Val Lys Phe Glu Asp Arg Asn Asp Leu 405 410 415 Val Phe
Lys Ile Arg Asn Ala Asn Glu Lys Arg Leu Leu Tyr Val Ala 420 425 430
Gln Ala Phe Arg Glu Gly Phe Ser Val Glu Glu Leu Tyr Glu Leu Cys 435
440 445 Lys Ile Asp Pro Trp Phe Leu Thr Gln Ile Lys Glu Ile Val Asp
Phe 450 455 460 Glu Glu Gln Ile Asp Met Asp Ile Leu Asn Asn Lys Ala
Leu Leu Arg 465 470 475 480 Lys Ala Lys Thr Met Gly Phe Ser Asp Lys
Met Ile Ala Leu Leu Val 485 490 495 Asn Leu Lys Asp Asn Leu Glu Leu
Ser Gln Asn Asp Ile Tyr Tyr Val 500 505 510 Arg Met Lys Gln Lys Ile
Ile Ala Glu Phe Ser Glu Val Asp Thr Cys 515 520 525 Ala Gly Glu Phe
Glu Ala Leu Thr Pro Tyr Leu Tyr Ser Ser Ile Asn 530 535 540 Val Ser
Glu Leu Thr Gln Ser Lys Asn Asp Ala Lys Asp Lys Lys Glu 545 550 555
560 Lys Lys Val Met Ile Ile Gly Gly Gly Pro Asn Arg Ile Gly Gln Gly
565 570 575 Ile Glu Phe Asp Tyr Ala Cys Val His Ala Ser Phe Ala Leu
Lys Asp 580 585 590 Met Gly Ile Lys Thr Ile Met Tyr Asn Cys Asn Pro
Glu Thr Val Ser 595 600 605 Thr Asp Tyr Asp Thr Ser Asp Ile Leu Tyr
Phe Glu Pro Ile Asp Phe 610 615 620 Glu His Leu Arg Ala Val Ile Glu
Arg Glu Lys Pro Asp Gly Val Ile 625 630 635 640 Val His Phe Gly Gly
Gln Thr Pro Leu Lys Phe Ala Lys Arg Leu Ser 645 650 655 Ala Phe Gly
Ala Lys Ile Ile Gly Thr Ser Ala Arg Val Ile Asp Met 660 665 670 Ala
Glu Asp Arg Lys Lys Phe Ala Glu Phe Ile Thr Lys Leu Gly Ile 675 680
685 Asn Gln Pro Lys Asn Ser Thr Ala Thr Ser Val Glu Glu Ala Val Leu
690 695 700 Lys Ala Ser Asp Ile Gly Tyr Pro Val Leu Val Arg Pro Ser
Tyr Val 705 710 715 720 Leu Gly Gly Arg Ala Met Arg Val Val Asn Asp
Glu Ala Glu Leu Arg 725 730 735 Leu Tyr Met Gln Glu Ala Val Asp Val
Ser Asp Lys Ser Pro Val Leu 740 745 750 Ile Asp Gln Phe Leu Asp Asn
Ala Thr Glu Ile Asp Val Asp Ala Ile 755 760 765 Cys Asp Gly Lys Asp
Val Tyr Val Ala Gly Ile Met Glu His Ile Glu 770 775 780 Glu Ala Gly
Ile His Ser Gly Asp Ser Ala Cys Ser Leu Pro Pro Cys 785 790 795 800
Asn Ile Asp Glu Lys Met Gln Glu Phe Ile Ala Gln Lys Thr Ala Asp 805
810 815 Ile Ala Leu Asn Leu Gly Val Val Gly Leu Leu Asn Ile Gln Phe
Ala 820 825 830 Leu His Asn Asn Glu Leu Tyr Met Ile Glu Val Asn Pro
Arg Ala Ser 835 840 845 Arg Thr Ile Pro Phe Val Ser Lys Ala Thr Gly
Ile Pro Leu Ala Lys 850 855 860 Val Ala Thr Arg Val Met Trp Gln Gly
Asn Leu Lys Glu Ala Leu Lys 865 870 875 880 Phe Tyr Asp Thr Phe Lys
Val Val Asn Phe Asp Thr Lys Ile Leu Arg 885 890 895 Pro Lys Thr Pro
Lys Tyr Met Ser Val Lys Glu Ala Val Phe Pro Phe 900 905 910 Ala Lys
Leu Ser Gly Ser Asp Leu Glu Leu Gly Pro Glu Met Arg Ser 915 920 925
Thr Gly Glu Val Met Gly Ile Ser Lys Asp Phe Ala Asn Ser Tyr Ala 930
935 940 Lys Ser Gln Ile Ala Ser Phe Asn His Leu Pro Glu Gln Gly Val
Val 945 950 955 960 Phe Ile Ser Leu Lys Asp Lys Asp Lys Lys Tyr Thr
Lys Lys Ile Ala 965 970 975 Ala Glu Tyr Val Lys Leu Gly Phe Lys Leu
Met Ala Thr Gly Gly Thr 980 985 990 Cys Lys Glu Ile Leu Glu Ser Gly
Phe Glu Cys Glu Leu Val His Lys 995 1000 1005 Ile Ser Glu Gly Arg
Pro Asn Val Glu Asp Lys Leu Lys Asn Gly 1010 1015 1020 Glu Ile His
Leu Val Ile Asn Thr Ser Asp Ser His Ser Phe Lys 1025 1030 1035 Gly
Asp Thr Lys Lys Ile Arg Glu Asn Ile Ile Arg Phe Lys Ile 1040 1045
1050 Pro Tyr Phe Thr Asn Leu Arg Ser Ala Leu Ala Gly Ala Lys Ser
1055 1060 1065 Ile Lys Ala Ile Gln Ser Lys Ser Cys Leu Asp Val Lys
Ser Leu 1070 1075 1080 Gln Glu Trp Leu Lys Ser 1085
251120PRTCampylobacter jejuni 25Met Lys Asn Ile Thr Leu Thr Lys Ile
Pro Ile Gly Glu Gly Lys Glu 1 5 10 15 Pro Cys Leu Asn Ser Lys Lys
Ile Val Leu Ser Leu Ala Thr Ile Ser 20 25 30 Phe Leu Ala Ser Cys
Ala Asn Ala Lys Leu Asn Ser Glu Ile Lys Thr 35 40 45 Tyr Asp Glu
Val Asn Lys Asn Val Lys Thr Arg Ser Ala Ser Val Tyr 50 55 60 Ser
Pro Gln Ala Lys Ile Asn Thr Thr Ile Asn Ser Leu His Asn Gln 65 70
75 80 Gln Val Thr Ile Thr Gly Asn Gly Thr Ser Asn Ser Leu Thr Ile
Gly 85 90 95 Ser Ser Gly Thr Leu Gly Ser Ile Gly Asn Thr Gly Lys
Ile Ile Tyr 100 105 110 Ala His Ala Asn Gly Ser Asn Thr Leu Thr Leu
Ala Asn Leu Thr Asn 115 120 125 Asn Arg Thr Ile Asn Gly Lys Ile Gly
Ile Glu Asn Asn Gly Asn Phe 130 135 140 Thr Gly Thr Ile Ala Val Asn
Thr Phe Glu Asn Thr Gly Gln Ile Asn 145 150 155 160 Gly Gln Ile Tyr
Met Gly Ile Trp Gly Asn Asn Ser Gly Thr Leu Asn 165 170 175 Ile Asp
Lys Phe Asp Asn Ser Gly Thr Ile Ile Asp Asn Asn Lys Gly 180 185 190
Val Phe Glu Gly Lys Asn Thr Asn Ile Gln Thr Phe Asn Asn Ser Gly 195
200 205 Phe Ile Ser Ala Asn Lys Gly Val Asp Ile Gly Asn Ile Gly Thr
Ile 210 215 220 Lys Asn Phe Asn Asn Asn Gly Thr Ile Gln Gly Ser Glu
Val Gly Val 225 230 235 240 Ala Ile Asn Thr Lys Ile Asp Thr Phe Thr
Asn Asn Gly Phe Ile Asn 245 250 255 Ser Pro Gly Ser Gly Gln Trp Asn
Asn Gly Ile Trp Ile Ser Ser Asn 260 265 270 Ala Thr Ile Glu Lys Leu
Val Asn Asn Gly Thr Ile Lys Gly Gly His 275 280 285 Ser Ala Ile Met
Val Thr Ser Gln His Ile Lys Thr Val Glu Asn Thr 290 295 300 Gly Ile
Ile His Ala Glu Gly Glu Trp Gly Ser Ser Ile Leu Leu Glu 305 310 315
320 Tyr Gly Gly Phe Ile Glu His Ile Ile Asn Thr Gly Thr Ile Ser Asn
325 330 335 Asn Asn Val Gly Ile Gly Ser Ala Tyr Gly Val Phe Gly Thr
Leu Thr 340 345 350 Ile Lys Asp Gly Gly Met Val Tyr Gly Lys Tyr Ser
Ala Ile Gly Val 355 360 365 Gly Arg Ser Gln Thr Leu Gly Asp Leu Tyr
Ile Asp Gly Arg Ser Asn 370 375 380 Asn Gly Thr Val Ser Gly Ile Tyr
Ser Glu Glu His Gly Ile Leu Leu 385 390 395 400 Glu Asn Asn Ser Arg
Thr Gln Lys Ile Glu Leu Lys Asn Gly Gly Ile 405 410 415 Ile Lys Gly
Asn Ile Asp Gly Ile Arg Leu Ile Asn Ser Ala Ser Leu 420 425 430 Ser
Gly Glu Met Ile Leu Ser Gly Glu Gly Ser Arg Val Glu Gly Gly 435 440
445 Arg Gly Val Gly Ile Leu Asn Arg Ser Gly Lys Ile Glu Gly Ser Ile
450 455 460 Lys Val Glu Asp Gly Ala Thr Val Thr Ala Thr Ser Asn Arg
Ala Ile 465 470 475 480 Ala Asn Ser Gly Ser Gly Ser Ile Thr Gly Gly
Ile Thr Val Ser Gly 485 490 495 Lys Asn Thr Lys Leu Glu Gly Asn Ile
Ile Asn Thr Gly Asn Ala Ser 500 505 510 Ile Gly Ser Asp Ile Lys Ile
Glu Gly Gly Ala Lys Val Glu Gly Gly 515 520 525 Leu Val Asn Gln Gly
Asn Gly Ser Ile Ser Gly Ser Val Gln Val Ser 530 535 540 Gly Gly Ser
Ser Ile Asp Ser Ile Thr Asn Glu Gly Asn Gly Ala Ile 545 550 555 560
Ser Gly Ser Ile Thr Val Tyr Lys Asp Ser Lys Leu Asp Ser Ile Thr 565
570 575 Asn Thr Ser Thr Ser Ser Thr Gly Ile Ser Gly Ser Ile Thr Asn
Asn 580 585 590 Ser Asp Asn Lys Leu Glu Ile Ser Asn Ser Gly Asn Ile
Gly Gly Lys 595 600 605 Ile Glu Ser Thr Gly Ser Ala Asp Met Val Ile
Ser Asn Ser Asn Gly 610 615 620 Gly Thr Ile Ser Gly Gly Ile Ser Ser
Ser Gly Ser Gly Ser Thr Ser 625 630 635 640 Ile Ser Asn Ser Gln Gly
Ser Thr Ile Asn Asn Gly Ile Thr Val Ser 645 650 655 Gly Ser Ala Gln
Val Glu Ile Ser Asn Gln Gly Ser Val Gly Lys Asp 660 665 670 Glu Asn
Gly Asn Thr Val Thr Asn Asn Gly Ser Gly Ser Val Gly Ile 675 680 685
Lys Asp Trp Leu Val Ser Thr Asp Lys Asn Thr Gly Lys Leu Asn Thr 690
695 700 Val Val Ile Gly Gly
Ser Arg Ala Phe Asn Val Lys Val Glu Asn Ile 705 710 715 720 Thr Val
Asp Gln Ser Asn Val Asp Leu Glu Glu Leu Asn Asp Ile Asn 725 730 735
Asn Ile Ile Ser Gly Val Asn Gln Asn Asn Ile Gly Asn Ile Gly Thr 740
745 750 Asn Gly Ser Gly Glu Ile Ser Leu Ser Phe Asp Pro Ile Thr Gly
Lys 755 760 765 Leu Thr Thr Asp Phe Asn Leu Asn Ala Ser Ile Ser Gly
Ala Thr Phe 770 775 780 Arg Ser Leu Ile Ser Thr Thr Ser Arg Arg Ser
Thr Phe Ile Asp Asn 785 790 795 800 Val Met Gly Asn Ser Met Gln Ser
Phe Ala Leu Ala Ser Ser Ser Lys 805 810 815 Ser Gln Ser Ile Ala Met
Ser Glu Lys Gly Asn Leu Tyr Ala Asp Ala 820 825 830 Ser Asp Tyr Ile
Lys Ser Asp Leu Asn Asn Gly Ser Tyr Gly Ser Asn 835 840 845 Lys Glu
His Ser Leu Phe Ile Leu Pro Tyr Thr Ser Ser Gln Asn Val 850 855 860
Glu Leu Ser Leu Asn Glu Glu Ser Lys Gly His Thr Lys Gly Thr Ile 865
870 875 880 Ile Gly Tyr Ser Thr Leu Lys Asp Ser Gly Ile Tyr Gly Val
Tyr Ala 885 890 895 Gly Tyr Glu Asp Thr Lys Met Gly Ser Thr Tyr Phe
Asp Ile Asn Asn 900 905 910 Arg Thr Tyr Tyr Ala Gly Leu Lys Tyr Phe
Asn Thr Leu Phe Thr Thr 915 920 925 Glu Lys Gly Gln Glu Val Tyr Ile
Lys Ala Gln Gly Lys Ala Ala Leu 930 935 940 Ile Lys Asn Asp Leu Thr
Glu Lys Ile Gly Asn Asn Glu Ala Lys Ala 945 950 955 960 Glu Pro Asn
Ser Tyr Ala Tyr Gly Val Asn Thr Ala Leu Gly Met Asn 965 970 975 Phe
Ile Ser Asn Lys Asp Ile Phe Ser Pro Glu Ile Gly Leu Ala Tyr 980 985
990 Glu Gly Gly Tyr Thr Glu Ala Phe Ser Met Lys Asp Thr Ile Gly Gln
995 1000 1005 Ala Thr Val Lys Gly Gly Glu Arg Thr Tyr Ala Asn Tyr
Leu Asn 1010 1015 1020 Leu Phe Ser Thr Lys Thr Ser Leu Thr Trp Phe
Arg Asp Trp Leu 1025 1030 1035 Pro Asn Leu Lys Thr Ser Val Glu Leu
Gly Ala Lys Phe Asn Ile 1040 1045 1050 Asn Pro Lys Val Glu Ala Glu
Ala Arg Phe Gly Asn Ile Lys Val 1055 1060 1065 Ser Asp Glu Phe Asp
Leu Pro Arg Val Gln Lys Phe Val Ser Thr 1070 1075 1080 Ser Phe Ile
Val Pro Val Asn Glu Ala Phe Tyr Phe Ser Leu Asn 1085 1090 1095 Tyr
Asn Gly Met Phe Asp Lys Asp Gly Asn Thr His Thr Gly Phe 1100 1105
1110 Ala Gln Phe Asn Tyr Leu Trp 1115 1120 261144PRTCampylobacter
jejuni 26Met Asn Lys Thr Ala Leu Thr Lys Thr Tyr Thr Lys Asp Ile
Gln Asn 1 5 10 15 Ser Cys Leu Asn Ser Lys Lys Ile Val Leu Ser Leu
Ala Thr Ile Ser 20 25 30 Phe Leu Ala Ser Cys Thr His Ala Thr Leu
Thr Pro Glu Ile Lys Thr 35 40 45 Tyr Glu Glu Thr Asn Arg His Ala
Lys Ala Arg Ser Gly Leu Gln Ser 50 55 60 Arg Asn Ser Asn Asn Glu
Thr Ile Asn Asn Leu Gln Thr Leu Thr Lys 65 70 75 80 Thr Ile Ser Asp
Thr Gly Asn Thr Leu Val Ile Glu Ser Ser Gly Thr 85 90 95 Ile Thr
Ile Ser Asn Asp Gly Gln Gln Ala Val Asn Phe Gln Pro Asn 100 105 110
Ser Ser Thr Ser Thr Phe Leu Asn Lys Gly Thr Leu Ile Gly Gly Asn 115
120 125 Asn Thr Ala Ser Val Gln Leu Gly Ala Ala Asn Gly Asn Asn Gly
Val 130 135 140 Ser Ile Glu Thr Phe Asn Asn Gln Gly Ile Ile Gly Asn
Gly Ser Ser 145 150 155 160 Lys Phe Gly Val Thr Val Phe Gly Gly Gly
Ser Lys Asp Asn Pro Lys 165 170 175 Ser Ile Ile Asn Asn Phe Ser Asn
Ser Gly Thr Ile His Ser Asn Thr 180 185 190 Gly Glu Ser Ile Tyr Phe
Gly Asn Ala Lys Ile Ser Ser Phe Val Asn 195 200 205 Ser Gly Thr Ile
Lys Ser Lys Gln Gly Ala Gly Val Asn Ile Ser Gln 210 215 220 Gly Thr
Ser Ile Glu Asn Phe Asn Asn Thr Gly Thr Gly Ile Ile Glu 225 230 235
240 Gly Lys Arg Met Gly Val Asn Val Arg Ser Thr Ile Asn Thr Phe Val
245 250 255 Asn Asp Gly Leu Ile Ala Ala Thr Asn Asp Gly Ile Gln Ile
Asn Ala 260 265 270 Asn Val Lys Thr Leu Ile Asn Lys Gly Thr Ile Lys
Gly Asp Ala Ile 275 280 285 Ser Ile Arg Ser Leu Gly Gly Thr Ile Glu
Thr Leu Thr Asn Glu Gly 290 295 300 Ile Met Tyr Gly Lys Ser Ala Gly
Ile Tyr Met Asn Arg Ser Leu Val 305 310 315 320 Lys Thr Leu Thr Asn
Ser Gly Thr Ile Asn Gln Asn Asn Ser Ala Thr 325 330 335 Trp Ser Ala
Gly Ile Lys Leu Glu Asn Gly Ser Ile Ile Glu Asn Ile 340 345 350 Ile
Asn Thr Gly Ser Ile Arg Ser Asn Ala Phe Gly Ile Ser Val Thr 355 360
365 Gly Gly Lys Phe Gly Thr Leu Thr Ile Lys Asp Gly Gly Met Val Tyr
370 375 380 Gly Lys Tyr Ser Ala Ile Gly Val Gly Arg Ser Gln Thr Leu
Gly Asp 385 390 395 400 Leu Tyr Ile Asp Gly Arg Ser Asn Asn Gly Thr
Val Ser Gly Ile Tyr 405 410 415 Ser Glu Glu His Gly Ile Leu Leu Glu
Asn Asn Ser Arg Thr Gln Lys 420 425 430 Ile Glu Leu Lys Asn Gly Gly
Ile Ile Lys Gly Asn Ile Asp Gly Ile 435 440 445 Arg Leu Ile Asn Ser
Ala Ser Leu Ser Gly Glu Met Ile Leu Ser Gly 450 455 460 Glu Gly Ser
Arg Val Glu Gly Gly Arg Gly Val Gly Ile Leu Asn Arg 465 470 475 480
Ser Gly Lys Ile Glu Gly Ser Ile Lys Val Glu Asp Gly Ala Thr Val 485
490 495 Thr Ala Thr Ser Asn Arg Ala Ile Ala Asn Ser Gly Ser Gly Ser
Ile 500 505 510 Thr Gly Gly Ile Thr Val Ser Gly Lys Asn Thr Lys Leu
Glu Gly Asn 515 520 525 Ile Ile Asn Thr Gly Asn Ala Ser Ile Gly Ser
Asp Ile Lys Ile Glu 530 535 540 Gly Gly Ala Lys Val Glu Gly Gly Leu
Val Asn Gln Gly Asn Gly Ser 545 550 555 560 Ile Ser Gly Ser Val Gln
Val Ser Gly Gly Ser Ser Ile Asp Ser Ile 565 570 575 Thr Asn Glu Gly
Asn Gly Ala Ile Ser Gly Ser Ile Thr Val Tyr Lys 580 585 590 Asp Ser
Lys Leu Asp Ser Ile Thr Asn Thr Ser Thr Ser Ser Thr Gly 595 600 605
Ile Ser Gly Ser Ile Thr Asn Asn Ser Asp Asn Lys Leu Glu Ile Ser 610
615 620 Asn Ser Gly Asn Ile Gly Gly Lys Ile Glu Ser Thr Gly Ser Ala
Asp 625 630 635 640 Met Val Ile Ser Asn Ser Asn Gly Gly Thr Ile Ser
Gly Gly Ile Ser 645 650 655 Ser Ser Gly Ser Gly Ser Thr Ser Ile Ser
Asn Ser Gln Gly Ser Thr 660 665 670 Ile Asn Asn Gly Ile Thr Val Ser
Gly Ser Ala Gln Val Glu Ile Ser 675 680 685 Asn Gln Gly Ser Val Gly
Lys Asp Glu Asn Gly Asn Thr Val Thr Asn 690 695 700 Asn Gly Ser Gly
Ser Val Gly Ile Lys Asp Trp Leu Val Ser Thr Asp 705 710 715 720 Lys
Asn Thr Gly Lys Leu Asn Thr Val Val Ile Gly Gly Ser Arg Ala 725 730
735 Phe Asn Val Lys Val Glu Asn Ile Thr Val Asp Gln Ser Asn Val Asp
740 745 750 Leu Glu Glu Leu Asn Asp Ile Asn Asn Ile Ile Ser Gly Val
Asn Gln 755 760 765 Asn Asn Ile Gly Asn Ile Gly Thr Asn Gly Ser Gly
Glu Ile Ser Leu 770 775 780 Ser Phe Asp Pro Ile Thr Gly Lys Leu Thr
Thr Asp Phe Asn Leu Asn 785 790 795 800 Ala Ser Ile Ser Gly Ala Thr
Phe Arg Ser Leu Ile Ser Thr Thr Ser 805 810 815 Arg Arg Ser Thr Phe
Ile Asp Asn Val Met Gly Asn Ser Met Gln Ser 820 825 830 Phe Ala Leu
Ala Ser Ser Ser Lys Ser Gln Ser Ile Ala Met Ser Glu 835 840 845 Lys
Gly Asn Leu Tyr Ala Asp Ala Ser Asp Tyr Ile Lys Ser Asp Leu 850 855
860 Asn Asn Gly Ser Tyr Gly Ser Asn Lys Glu His Ser Leu Phe Ile Leu
865 870 875 880 Pro Tyr Thr Ser Ser Gln Asn Val Glu Leu Ser Leu Asn
Glu Glu Ser 885 890 895 Lys Gly His Thr Lys Gly Thr Ile Ile Gly Tyr
Ser Thr Leu Lys Asp 900 905 910 Ser Gly Ile Tyr Gly Val Tyr Ala Gly
Tyr Glu Asp Thr Lys Met Gly 915 920 925 Ser Thr Tyr Phe Asp Ile Asn
Asn Arg Thr Tyr Tyr Ala Gly Leu Lys 930 935 940 Tyr Phe Asn Thr Leu
Phe Thr Thr Glu Lys Gly Gln Glu Val Tyr Ile 945 950 955 960 Lys Ala
Gln Gly Lys Ala Ala Leu Ile Lys Asn Asp Leu Thr Glu Lys 965 970 975
Ile Gly Asn Asn Glu Ala Lys Ala Glu Pro Asn Ser Tyr Ala Tyr Gly 980
985 990 Val Asn Thr Ala Leu Gly Met Asn Phe Ile Ser Asn Lys Asp Ile
Phe 995 1000 1005 Ser Pro Glu Ile Gly Leu Ala Tyr Glu Gly Gly Tyr
Thr Glu Ala 1010 1015 1020 Phe Ser Met Lys Asp Thr Ile Gly Gln Ala
Thr Val Lys Gly Gly 1025 1030 1035 Glu Arg Thr Tyr Ala Asn Tyr Leu
Asn Leu Phe Ser Thr Lys Thr 1040 1045 1050 Ser Leu Thr Trp Phe Arg
Asp Trp Leu Pro Asn Leu Lys Thr Ser 1055 1060 1065 Val Glu Leu Gly
Ala Lys Phe Asn Ile Asn Pro Lys Val Glu Ala 1070 1075 1080 Glu Ala
Arg Phe Gly Asn Ile Lys Val Ser Asp Glu Phe Asp Leu 1085 1090 1095
Pro Arg Val Gln Lys Phe Val Ser Thr Ser Phe Ile Val Pro Val 1100
1105 1110 Asn Glu Ala Phe Tyr Phe Ser Leu Asn Tyr Asn Gly Met Phe
Asp 1115 1120 1125 Lys Asp Gly Asn Thr His Thr Gly Phe Ala Gln Phe
Asn Tyr Leu 1130 1135 1140 Trp 271186PRTCampylobacter jejuni 27Met
Gly Lys Ile Met Lys Thr Met Asp Gly Asn Glu Ala Ala Ala Tyr 1 5 10
15 Ala Ala Tyr Ala Phe Thr Glu Val Ala Gly Ile Tyr Pro Ile Thr Pro
20 25 30 Ser Ser Pro Met Ala Asp Tyr Thr Asp Met Trp Ala Ala Ala
Gly Lys 35 40 45 Lys Asn Leu Phe Gly Val Pro Val Lys Ile Val Glu
Met Gln Ser Glu 50 55 60 Ala Gly Ala Ala Gly Ser Val His Gly Ser
Leu Gln Ala Gly Ala Leu 65 70 75 80 Thr Thr Thr Tyr Thr Ala Ser Gln
Gly Leu Leu Leu Lys Ile Pro Asn 85 90 95 Met Tyr Lys Ile Ala Gly
Gln Leu Leu Pro Cys Val Ile His Val Ala 100 105 110 Ala Arg Ser Leu
Ala Ala Gln Ala Leu Ser Ile Phe Gly Asp His Gln 115 120 125 Asp Ile
Tyr Ala Ala Arg Gln Ile Gly Phe Ala Met Leu Cys Ser His 130 135 140
Ser Val Gln Glu Thr Met Asp Leu Ala Gly Val Ala His Leu Ala Ala 145
150 155 160 Ile Lys Gly Arg Val Pro Phe Leu His Phe Phe Asp Gly Phe
Arg Thr 165 170 175 Ser His Glu Ile Gln Lys Val Glu Val Met Asp Tyr
Ala His Phe Asp 180 185 190 Arg Leu Leu Asp Arg Glu Ala Leu Leu Glu
Phe Arg Asn Asn Ala Leu 195 200 205 Asn Pro Glu Asn Pro Lys Thr Arg
Gly Thr Ala Gln Asn Asp Asp Ile 210 215 220 Tyr Phe Gln Thr Arg Glu
Val Ser Asn Arg Phe Tyr Asp Ala Leu Pro 225 230 235 240 Asp Val Val
Asn Glu Tyr Met Gln Glu Ile Ser Lys Ile Thr Gly Arg 245 250 255 Glu
Tyr Lys Pro Phe Thr Tyr Tyr Gly His Lys Glu Pro Glu Cys Val 260 265
270 Ile Val Ala Met Gly Ser Val Thr Gln Ala Leu Glu Glu Val Val Asp
275 280 285 Tyr Leu Asn Ala Lys Gly Glu Lys Val Gly Ile Leu Lys Val
Tyr Leu 290 295 300 Tyr Arg Pro Phe Ser Leu Lys Tyr Phe Phe Asp Val
Met Pro Lys Ser 305 310 315 320 Val Lys Lys Ile Ala Val Leu Asp Arg
Thr Lys Glu Pro Gly Ser Leu 325 330 335 Gly Glu Pro Leu Tyr Leu Asp
Val Lys Ser Ala Phe Tyr Gly Arg Glu 340 345 350 Asn Ala Pro Val Ile
Val Gly Gly Arg Tyr Gly Leu Ser Ser Lys Asp 355 360 365 Val Asp Pro
Ala Gln Met Ile Ala Val Phe Glu Asn Leu Lys Leu Asp 370 375 380 Asn
Pro Lys Asp Gly Phe Thr Val Gly Ile Ile Asp Asp Val Thr His 385 390
395 400 Thr Ser Leu Ser Thr Gly Glu Lys Ile Ser Leu Gly Asp Glu Ser
Thr 405 410 415 Ile Glu Cys Leu Phe Tyr Gly Leu Gly Ala Asp Gly Thr
Val Gly Ala 420 425 430 Asn Lys Asn Ser Ile Lys Ile Ile Gly Asp Lys
Thr Asp Phe Tyr Ala 435 440 445 Gln Ala Tyr Phe Ala Tyr Asp Ser Lys
Lys Ser Gly Gly Tyr Thr Arg 450 455 460 Ser His Leu Arg Phe Ser Lys
Lys Pro Ile Arg Ser Thr Tyr Leu Val 465 470 475 480 Ser Thr Pro His
Phe Ile Ala Cys Ser Val Ala Ala Tyr Leu Glu Ile 485 490 495 Tyr Asp
Val Leu Ala Gly Ile Arg Lys Gly Gly Thr Phe Leu Leu Asn 500 505 510
Ser Ile Trp Asn Ala Glu Glu Thr Ile Arg Gln Leu Pro Asp Ala Val 515
520 525 Lys Lys Thr Leu Ala Glu Lys Glu Val Asn Phe Tyr Ile Ile Asn
Ala 530 535 540 Thr Lys Leu Ala Arg Asp Ile Gly Leu Gly Asn Arg Thr
Asn Thr Ile 545 550 555 560 Met Gln Ser Ala Phe Phe Lys Leu Ala Lys
Ile Ile Pro Tyr Glu Asp 565 570 575 Ala Gln Lys Tyr Met Lys Glu Leu
Ala Tyr Lys Ser Tyr Ser Lys Lys 580 585 590 Gly Asp Ala Ile Val Glu
Met Asn Tyr Lys Ala Ile Asp Val Gly Ala 595 600 605 Asp Gly Leu Val
Lys Val Glu Val Asp Pro Asn Trp Lys Asn Leu Glu 610 615 620 Leu Lys
Glu Lys Glu Gln Thr Asn Ala Tyr Lys Gly Thr Glu Phe Val 625 630 635
640 Glu Lys Ile Val Lys Pro Met Asn Ala Ala Lys Gly Asp Asp Leu Pro
645 650 655 Val Ser Ala Phe Leu Gly Tyr Glu Asp Gly Ser Phe Glu His
Gly Thr 660 665 670 Thr Glu Tyr Glu Lys Arg Gly Val Gly Val Met Val
Pro Arg Trp Ile 675 680 685 Glu Ala Asn Cys Ile Gln Cys Asn Gln Cys
Ala Ser Val Cys Pro His 690 695 700 Ala Val Ile Arg Pro Phe Leu Ile
Asn Asp Glu Glu Met Ala Asn Ala 705 710 715 720 Pro Arg Gly Val Lys
Asp His Ala Leu Glu Ala Lys Gly Thr Lys Gly
725 730 735 Glu Lys Leu Ser Phe Lys Ile Gln Val Ser Pro Leu Asp Cys
Thr Gly 740 745 750 Cys Glu Leu Cys Val His Glu Cys Pro Thr Lys Glu
Lys Ser Leu Val 755 760 765 Met Val Pro Leu Gln Glu Glu Met Asp Phe
Gly Glu Gln Glu Asn Ala 770 775 780 Asp Tyr Leu Phe Lys Glu Ile Thr
Tyr Lys Asp Asp Ile Leu Asn Lys 785 790 795 800 Glu Thr Thr Lys Gly
Ala Gln Phe Ala Gln Pro Leu Phe Glu Phe His 805 810 815 Gly Ala Cys
Pro Gly Cys Gly Glu Thr Pro Tyr Ile Thr Leu Ile Thr 820 825 830 Arg
Leu Phe Gly Glu Arg Met Ile Val Ala Asn Ala Thr Gly Cys Ser 835 840
845 Ser Ile Tyr Gly Gly Ser Ala Pro Ser Thr Pro Tyr Arg Lys Ser Val
850 855 860 Lys Asn Gly His Gly Pro Ala Trp Gly Asn Ser Leu Phe Glu
Asp Asn 865 870 875 880 Ala Glu Phe Gly Leu Gly Met Lys Ile Ala Thr
Glu Asn Thr Arg His 885 890 895 Arg Ile Glu His Ile Met Asn Glu Ser
Met Gln Glu Val Pro Asn Ala 900 905 910 Leu Ser Ala Leu Phe Lys Asp
Trp Ile Ala Asn Lys Asp Asn Gly Ala 915 920 925 Met Ser Val Glu Ile
Lys Asp Lys Met Ile Pro Ile Leu Glu Gln Asn 930 935 940 Lys Asn Ile
Lys Ala Val Gln Asp Ile Leu Glu Leu Lys Gln Tyr Leu 945 950 955 960
Ser Lys Lys Ser His Trp Ile Phe Gly Gly Asp Gly Trp Ala Tyr Asp 965
970 975 Ile Gly Tyr Gly Gly Leu Asp His Val Leu Ala Ser Gly Glu Asn
Val 980 985 990 Asn Ile Leu Val Leu Asp Thr Glu Val Tyr Ser Asn Thr
Gly Gly Gln 995 1000 1005 Ser Ser Lys Ser Ser Arg Thr Gly Ala Val
Ala Gln Phe Ala Ala 1010 1015 1020 Ala Gly Lys Pro Ile Gln Lys Lys
Asp Leu Gly Gln Ile Ala Met 1025 1030 1035 Thr Tyr Gly Tyr Ile Phe
Val Ala Gln Val Asn Ser Thr Ala Asn 1040 1045 1050 Tyr Thr His Leu
Ile Lys Ala Ile Thr Ala Ala Glu Ala Tyr Asp 1055 1060 1065 Gly Pro
Ser Leu Val Ile Cys Tyr Ser Pro Cys Ile Ala His Gly 1070 1075 1080
Ile Lys Gly Gly Leu Gly Tyr Ser Gly Glu Gln Gly Glu Leu Ala 1085
1090 1095 Thr Lys Cys Gly Tyr Trp Pro Leu Tyr Thr Phe Asp Pro Arg
Leu 1100 1105 1110 Glu Glu Gln Gly Lys Asn Pro Leu Thr Leu Thr Gly
Lys Glu Pro 1115 1120 1125 Asp Trp Asp Leu Tyr Glu Gln Phe Leu Met
Asn Glu Val Arg Tyr 1130 1135 1140 Asn Ser Leu Lys Lys Ala Asn Pro
Glu His Ala Ala Glu Leu Phe 1145 1150 1155 Glu Arg Asn Lys Lys Asp
Ala Gln Arg Arg Tyr Arg Gln Leu Lys 1160 1165 1170 Arg Ile Ala Met
Ala Asp Tyr Ser Asn Glu Val Glu Ser 1175 1180 1185
281378PRTCampylobacter jejuni 28Met Cys Asp Met Leu Asp Asn Lys Leu
Gly Asn Arg Leu Arg Val Asp 1 5 10 15 Phe Ser Asn Ile Ser Lys Gln
Ile Glu Ile Pro Asn Leu Leu Gln Leu 20 25 30 Gln Lys Lys Ser Phe
Asp Tyr Phe Leu Asn Leu Asp Asn Gly Glu Ser 35 40 45 Gly Ile Glu
Lys Val Phe Lys Ser Ile Phe Pro Ile His Asp Pro Gln 50 55 60 Asn
Arg Leu Ser Leu Glu Tyr Val Ser Ser Glu Ile Gly Lys Pro Lys 65 70
75 80 Tyr Thr Ile Arg Glu Cys Met Glu Arg Gly Leu Thr Tyr Ser Val
Asn 85 90 95 Leu Lys Met Lys Ile Arg Leu Thr Leu His Glu Lys Asp
Glu Lys Thr 100 105 110 Gly Glu Lys Val Gly Val Lys Asp Ile Lys Glu
Gln Glu Ile Tyr Ile 115 120 125 Arg Glu Ile Pro Leu Met Thr Asp Arg
Val Ser Phe Ile Ile Asn Gly 130 135 140 Val Glu Arg Val Val Val Asn
Gln Leu His Arg Ser Pro Gly Val Ile 145 150 155 160 Phe Lys Glu Glu
Glu Ser Ser Thr Val Ala Asn Lys Leu Val Tyr Thr 165 170 175 Ala Gln
Ile Ile Pro Asp Arg Gly Ser Trp Leu Tyr Phe Glu Tyr Asp 180 185 190
Ala Lys Asp Val Leu Tyr Val Arg Ile Asn Lys Arg Arg Lys Val Pro 195
200 205 Val Thr Met Leu Phe Arg Ala Leu Gly Tyr Lys Lys Gln Asp Ile
Ile 210 215 220 Lys Leu Phe Tyr Pro Ile Gln Thr Ile His Val Lys Lys
Asp Lys Phe 225 230 235 240 Leu Thr Glu Phe Asn Pro Asn Asp Phe Met
Asp Arg Ile Glu Tyr Asp 245 250 255 Ile Lys Asp Glu Lys Gly Lys Ile
Val His Gln Ala Gly Lys Arg Leu 260 265 270 Thr Lys Lys Lys Ala Glu
Gln Leu Ile Lys Asp Gly Leu Lys Trp Ile 275 280 285 Glu Tyr Pro Val
Glu Ile Leu Leu Asn Arg Tyr Leu Ala Asn Pro Ile 290 295 300 Ile Asp
Lys Glu Ser Gly Glu Val Leu Phe Asp Ser Leu Thr Leu Leu 305 310 315
320 Asp Glu Ser Lys Leu Ala Lys Ile Lys Glu Gln Lys Ser Phe Asp Ile
325 330 335 Ala Asn Asp Leu Ala Asn Gly Val Asp Ala Ala Ile Ile Asn
Ser Phe 340 345 350 Ala Gln Asp Gly Glu Thr Leu Lys Leu Leu Lys Gln
Ser Glu Asn Ile 355 360 365 Asp Asp Glu Asn Asp Leu Ala Ala Ile Arg
Ile Tyr Lys Val Met Arg 370 375 380 Pro Gly Glu Pro Val Val Lys Asp
Ala Ala Lys Ala Phe Val Asn Asp 385 390 395 400 Leu Phe Phe Asn Pro
Glu Arg Tyr Asp Leu Thr Lys Val Gly Arg Met 405 410 415 Lys Met Asn
His Lys Leu Gly Leu Glu Val Pro Glu Tyr Val Thr Val 420 425 430 Leu
Thr Asn Glu Asp Ile Ile Lys Thr Ala Lys Tyr Leu Ile Lys Val 435 440
445 Lys Asn Gly Lys Gly His Ile Asp Asp Arg Asp His Leu Gly Asn Arg
450 455 460 Arg Ile Arg Ser Ile Gly Glu Leu Leu Ala Asn Glu Leu His
Leu Gly 465 470 475 480 Leu Ala Lys Met Gln Lys Ala Ile Arg Asp Lys
Phe Thr Ser Leu Asn 485 490 495 Ala Asp Leu Asp Lys Val Met Pro Tyr
Asp Leu Ile Asn Pro Lys Met 500 505 510 Ile Thr Thr Thr Ile Ile Glu
Phe Phe Thr Gly Gly Gln Leu Ser Gln 515 520 525 Phe Met Asp Gln Thr
Asn Pro Leu Ser Glu Val Thr His Lys Arg Arg 530 535 540 Leu Ser Ala
Leu Gly Glu Gly Gly Leu Val Lys Glu Arg Ala Gly Phe 545 550 555 560
Glu Val Arg Asp Val His Ala Thr His Tyr Gly Arg Ile Cys Pro Val 565
570 575 Glu Thr Pro Glu Gly Gln Asn Ile Gly Leu Ile Asn Thr Leu Ser
Thr 580 585 590 Tyr Ala Lys Val Asn Glu Leu Gly Phe Val Glu Ala Pro
Tyr Arg Lys 595 600 605 Val Val Asn Gly Lys Val Thr Asn Glu Val Val
Tyr Leu Thr Ala Thr 610 615 620 Gln Glu Glu Gly Leu Phe Ile Ala Pro
Ala Ser Thr Lys Val Asp Ala 625 630 635 640 Lys Gly Asn Ile Val Glu
Glu Phe Val Glu Ala Arg Gln Asp Gly Glu 645 650 655 Thr Ile Leu Ala
Arg Arg Glu Glu Val Gln Leu Ile Asp Leu Cys Ser 660 665 670 Gly Met
Val Val Gly Val Ala Ala Ser Leu Ile Pro Phe Leu Glu His 675 680 685
Asp Asp Ala Asn Arg Ala Leu Met Gly Ser Asn Met Gln Arg Gln Ala 690
695 700 Val Pro Leu Leu Thr Ala Ser Ala Pro Ile Val Gly Thr Gly Met
Glu 705 710 715 720 Gln Ile Ile Ala Arg Asp Ala Trp Glu Ala Val Lys
Ala Lys Arg Gly 725 730 735 Gly Val Val Glu Lys Val Asp Asn Lys Ser
Ile Phe Ile Leu Gly Glu 740 745 750 Asp Asp Lys Gly Pro Phe Ile Asp
His Tyr Thr Met Glu Lys Asn Leu 755 760 765 Arg Thr Asn Gln Asn Thr
Asn Tyr Ile Gln His Pro Ile Val Lys Lys 770 775 780 Gly Asp Ile Val
Lys Ala Gly Gln Ile Ile Ala Asp Gly Pro Ser Met 785 790 795 800 Asp
Gln Gly Glu Leu Ala Ile Gly Lys Asn Ala Leu Ile Ala Phe Met 805 810
815 Pro Trp Asn Gly Tyr Asn Tyr Glu Asp Ala Ile Val Val Ser Glu Arg
820 825 830 Ile Ile Arg Glu Asp Thr Phe Thr Ser Val His Ile Tyr Glu
Lys Glu 835 840 845 Ile Glu Ala Arg Glu Leu Lys Asp Gly Ile Glu Glu
Ile Thr Lys Asp 850 855 860 Ile Pro Asn Val Lys Glu Glu Asp Val Ala
His Leu Asp Glu Ser Gly 865 870 875 880 Ile Ala Lys Ile Gly Thr His
Ile Lys Pro Gly Met Ile Leu Val Gly 885 890 895 Lys Val Ser Pro Lys
Gly Glu Val Lys Pro Thr Pro Glu Glu Arg Leu 900 905 910 Leu Arg Ala
Ile Phe Gly Glu Lys Ala Gly His Val Val Asn Lys Ser 915 920 925 Leu
Tyr Ala Thr Ala Ser Leu Glu Gly Val Val Val Asp Val Lys Ile 930 935
940 Phe Thr Lys Lys Gly Tyr Glu Lys Asp Asp Arg Ala Ile Lys Ser Tyr
945 950 955 960 Asp Lys Glu Lys Met Ala Leu Glu Lys Glu His His Asp
Arg Leu Leu 965 970 975 Met Met Asp Arg Glu Glu Met Leu Arg Val Cys
Ala Leu Leu Ser Lys 980 985 990 Ala Ser Leu Asn Ser Asp Gln Lys Ile
Gly Asp Lys Asn Tyr Lys Lys 995 1000 1005 Gly Gln Thr Ala Asp Ile
Ser Glu Leu Glu Lys Ile Asn Arg Phe 1010 1015 1020 Thr Leu Thr Thr
Leu Ile Lys Ala Tyr Ser Lys Glu Ile Gln Lys 1025 1030 1035 Glu Tyr
Asp Asp Leu Lys Asn His Phe Gln Asn Glu Lys Lys Lys 1040 1045 1050
Leu Lys Ala Glu His Asp Glu Lys Leu Glu Ile Leu Glu Lys Asp 1055
1060 1065 Asp Ile Leu Pro Ser Gly Val Ile Lys Leu Val Lys Val Tyr
Ile 1070 1075 1080 Ala Thr Lys Arg Lys Leu Lys Val Gly Asp Lys Met
Ala Gly Arg 1085 1090 1095 His Gly Asn Lys Gly Ile Val Ser Thr Ile
Val Pro Glu Val Asp 1100 1105 1110 Met Pro Tyr Leu Pro Asn Gly Lys
Ser Val Asp Ile Ala Leu Asn 1115 1120 1125 Pro Leu Gly Val Pro Ser
Arg Met Asn Ile Gly Gln Ile Leu Glu 1130 1135 1140 Ser His Leu Gly
Leu Val Gly Leu Arg Leu Gly Asp Gln Ile Gln 1145 1150 1155 Glu Ile
Phe Asp Arg Lys Gln Lys Asp Phe Leu Lys Glu Leu Arg 1160 1165 1170
Ala Lys Ile Leu Glu Ile Cys Ser Ile Pro Arg Leu Ala Asn Glu 1175
1180 1185 Lys Glu Phe Ile Lys Ser Leu Ser Asp Glu Glu Leu Leu Asn
Tyr 1190 1195 1200 Ala Arg Asp Trp Ser Lys Gly Val Lys Phe Ser Thr
Pro Val Phe 1205 1210 1215 Glu Gly Val Asn Ile Glu Glu Phe Ser Lys
Leu Phe Lys Met Ala 1220 1225 1230 Lys Ile Asp Met Asp Gly Lys Thr
Glu Leu Tyr Asp Gly Arg Thr 1235 1240 1245 Gly Glu Lys Ile Ala Glu
Arg Val His Val Gly Cys Met Tyr Met 1250 1255 1260 Leu Lys Leu His
His Leu Val Asp Glu Lys Val His Ala Arg Ser 1265 1270 1275 Thr Gly
Pro Tyr Ser Leu Val Thr Gln Gln Pro Val Gly Gly Lys 1280 1285 1290
Ala Leu Phe Gly Gly Gln Arg Phe Gly Glu Met Glu Val Trp Ala 1295
1300 1305 Leu Glu Ala Tyr Gly Ala Ala His Thr Leu Arg Glu Met Leu
Thr 1310 1315 1320 Ile Lys Ser Asp Asp Val Glu Gly Arg Phe Ser Ala
Tyr Lys Ala 1325 1330 1335 Leu Thr Lys Gly Glu Asn Val Pro Ala Thr
Gly Ile Pro Glu Thr 1340 1345 1350 Phe Phe Val Leu Thr Asn Glu Leu
Lys Ser Leu Ala Leu Asp Val 1355 1360 1365 Glu Ile Phe Asp Lys Asp
Glu Asp Asn Glu 1370 1375 291496PRTCampylobacter jejuni 29Met Asp
Leu Glu Asn Ile Leu Glu Asn Asn Gln Ser Ile Gly Leu Tyr 1 5 10 15
His Pro Lys Asn Glu His Asp Ala Cys Gly Ile Ala Ala Val Ala Asn 20
25 30 Ile Arg Gly Ile Ala Ser Tyr Lys Val Ile Cys Asp Ala Leu Glu
Ile 35 40 45 Leu Met Asn Leu Glu His Arg Gly Gly Thr Gly Ala Glu
Glu Asn Ser 50 55 60 Gly Asp Gly Ala Gly Ile Leu Ile Gln Ile Pro
His Asp Phe Phe Lys 65 70 75 80 Thr Gln Glu Leu Gly Phe Glu Leu Pro
Lys Lys Gly Asp Tyr Ala Val 85 90 95 Ala Gln Met Phe Leu Ser Pro
Asn Thr Asp Ala Lys Glu Glu Ala Lys 100 105 110 Glu Ile Phe Leu Gln
Gly Leu Lys Asp Lys Lys Leu Glu Phe Leu Gly 115 120 125 Phe Arg Glu
Val Pro Phe Asn Pro Ser Asp Ile Gly Ala Ser Ala Leu 130 135 140 Lys
Ala Met Pro Tyr Phe Leu Gln Ala Phe Val Lys Lys Pro Ser Lys 145 150
155 160 Ile Ser Ala Gly Leu Glu Phe Glu Arg Val Leu Tyr Ser Thr Arg
Arg 165 170 175 Leu Ile Glu Lys Arg Ala Ile Asn Val Pro Lys Phe Tyr
Phe Ser Ser 180 185 190 Phe Ser Ser Arg Thr Ile Val Tyr Lys Gly Met
Leu Leu Ser Thr Gln 195 200 205 Leu Ser Asp Phe Tyr Leu Asp Phe Lys
Asp Val Asn Met Lys Ser Ala 210 215 220 Ile Ala Leu Val His Ser Arg
Phe Ser Thr Asn Thr Phe Pro Ser Trp 225 230 235 240 Glu Arg Ala His
Pro Asn Arg Tyr Met Val His Asn Gly Glu Ile Asn 245 250 255 Thr Ile
Arg Gly Asn Val Asp Ser Ile Arg Ala Arg Glu Gly Leu Met 260 265 270
Gln Ser Glu Tyr Phe Glu Asn Leu Asp Glu Ile Phe Pro Ile Ile Ala 275
280 285 Lys Leu Ser Ser Asp Ser Ala Met Phe Asp Asn Thr Leu Glu Phe
Leu 290 295 300 Ala Leu Asn Gly Arg Thr Leu Glu Glu Ala Phe Met Met
Met Val Pro 305 310 315 320 Glu Pro Trp His Lys Asn Glu Asn Met Glu
Ser Lys Lys Arg Ala Phe 325 330 335 Tyr Glu Tyr His Ser Leu Leu Met
Glu Pro Trp Asp Gly Pro Ala Ala 340 345 350 Ile Val Phe Thr Asp Gly
Val Ile Met Gly Ala Ser Leu Asp Arg Asn 355 360 365 Gly Phe Arg Pro
Ser Arg Tyr Tyr Leu Thr Lys Asp Asp Met Leu Ile 370 375 380 Leu Ser
Ser Glu Thr Gly Ala Leu Lys Leu Asp Glu Lys Asn Ile Lys 385 390 395
400 Ala Lys Lys Arg Leu Glu Pro Gly Lys Leu Leu Leu Val Asp Thr Ala
405 410 415 Arg Gly Arg Val Ile Ala Asp Asn Glu Ile Lys Glu His Tyr
Ala Asn 420 425 430 Ala
Lys Pro Tyr Lys Lys Trp Leu Lys Asn Leu Val Glu Leu Glu Lys 435 440
445 Gln Lys Ser Gly Val Tyr Lys His Gln Phe Leu Lys Glu Asp Glu Val
450 455 460 Leu Lys Leu Gln Lys Ala Phe Gly Trp Ser Tyr Asp Glu Leu
Lys Met 465 470 475 480 Ser Val Ala Ala Met Ala Gln Asn Gly Lys Glu
Ala Ile Ala Ala Met 485 490 495 Gly Val Asp Thr Pro Leu Ala Ile Leu
Ser Lys Thr Tyr Gln Pro Leu 500 505 510 Tyr Asn Tyr Phe Lys Gln Leu
Phe Ala Gln Val Thr Asn Pro Pro Leu 515 520 525 Asp Ala Ile Arg Glu
Glu Ile Val Thr Ser Thr Arg Ile Tyr Leu Gly 530 535 540 Ser Glu Gly
Asn Leu Leu Lys Pro Asp Glu Asn Asn Ala Lys Arg Val 545 550 555 560
Lys Ile Ala Leu Pro Val Ile Ser Asn Glu Glu Leu Phe Glu Val Lys 565
570 575 Ala Leu Asn Lys Phe Gln Val Lys Glu Phe Ser Ile Leu Tyr Asp
Tyr 580 585 590 Ser Lys Lys Thr Leu Glu Lys Ala Leu Asp Glu Leu Cys
Val Lys Ile 595 600 605 Glu Asp Glu Val Lys Lys Gly Val Ser Ile Ile
Ile Leu Ser Asp Lys 610 615 620 Gly Val Asp Glu Lys Asn Ala Tyr Ile
Pro Ala Leu Leu Ala Val Ser 625 630 635 640 Gly Val His Asn His Leu
Val Arg Lys Asn Leu Arg Thr His Thr Ser 645 650 655 Leu Ile Ile Glu
Ser Gly Glu Pro Arg Glu Ile His His Phe Ala Cys 660 665 670 Leu Leu
Gly Tyr Gly Ala Thr Val Ile Asn Pro Tyr Leu Val Tyr Glu 675 680 685
Ser Ile Gln Lys Leu Ile Ala Asn Lys Asp Leu Asn Leu Ser Tyr Glu 690
695 700 Lys Ala Val Glu Asn Phe Ile Lys Ala Ser Ser Ser Gly Ile Val
Lys 705 710 715 720 Ile Ala Ser Lys Met Gly Val Ser Thr Leu Gln Ser
Tyr Asn Gly Ser 725 730 735 Ala Leu Phe Glu Cys Leu Gly Leu Ser Ser
Lys Val Ile Asp Lys Tyr 740 745 750 Phe Thr Ser Thr Thr Ser Arg Ile
Glu Gly Met Asp Leu Glu Asp Phe 755 760 765 Glu Lys Glu Leu Ile Ala
Leu His Lys His Ala Phe Asn Asp Thr His 770 775 780 Lys Ala Leu Asp
Ser Lys Gly Ile His Gly Phe Arg Ser Ala Lys Glu 785 790 795 800 Glu
His Leu Ile Asp Pro Leu Val Ile Phe Asn Leu Gln Gln Ala Cys 805 810
815 Arg Asn Lys Asp Tyr Lys Ser Phe Lys Lys Tyr Ser Ala Leu Val Asp
820 825 830 Glu Lys Gln Val Asn Leu Arg Ser Leu Met Glu Phe Asp Phe
Ser Glu 835 840 845 Ala Ile Ser Ile Asp Lys Val Glu Ser Val Glu Ser
Ile Val Lys Arg 850 855 860 Phe Arg Thr Gly Ala Met Ser Tyr Gly Ser
Ile Ser Lys Glu Ala His 865 870 875 880 Glu Cys Leu Ala Gln Ala Met
Asn Lys Ile Gly Ala Lys Ser Asn Ser 885 890 895 Gly Glu Gly Gly Glu
Asp Glu Glu Arg Tyr Glu Ile Lys Glu Gly Val 900 905 910 Asp Lys Asn
Ser Ala Ile Lys Gln Val Ala Ser Gly Arg Phe Gly Val 915 920 925 Asp
Leu Asn Tyr Leu Ser His Ala Lys Glu Ile Gln Ile Lys Val Ala 930 935
940 Gln Gly Ala Lys Pro Gly Glu Gly Gly Gln Leu Met Gly Phe Lys Val
945 950 955 960 Tyr Pro Trp Ile Ala Lys Ala Arg His Ser Thr Ala Gly
Val Thr Leu 965 970 975 Ile Ser Pro Pro Pro His His Asp Ile Tyr Ser
Ile Glu Asp Leu Ala 980 985 990 Gln Leu Ile Tyr Asp Leu Lys His Ala
Asn Lys Asp Ala Lys Ile Ser 995 1000 1005 Val Lys Leu Val Ser Glu
Asn Gly Ile Gly Thr Val Ala Ala Gly 1010 1015 1020 Val Ala Lys Ala
Gly Ala Asn Leu Ile Leu Val Ser Gly Tyr Asp 1025 1030 1035 Gly Gly
Thr Gly Ala Ser Pro Arg Thr Ser Ile Pro His Ala Gly 1040 1045 1050
Ile Pro Trp Glu Leu Gly Leu Ala Glu Thr His Gln Thr Leu Ile 1055
1060 1065 Leu Asn Lys Leu Arg Asp Arg Val Arg Leu Glu Thr Asp Gly
Lys 1070 1075 1080 Leu Met Asn Gly Arg Asp Leu Ala Ile Ala Ala Leu
Leu Gly Ala 1085 1090 1095 Glu Glu Phe Gly Phe Ala Thr Ala Pro Leu
Ile Val Leu Gly Cys 1100 1105 1110 Thr Met Met Arg Val Cys His Leu
Asn Thr Cys Pro Phe Gly Ile 1115 1120 1125 Ala Thr Gln Asp Thr Glu
Leu Arg Asp Arg Phe Lys Gly Lys Val 1130 1135 1140 Asp Asp Val Ile
Asn Phe Met Tyr Phe Ile Ala Glu Glu Leu Arg 1145 1150 1155 Glu Tyr
Met Ala Arg Leu Gly Phe Glu Arg Leu Asp Asp Met Ile 1160 1165 1170
Gly Arg Val Asp Lys Leu Arg Gln Lys Ser Val Gln Gly Lys Ala 1175
1180 1185 Gly Lys Leu Asn Leu Asp Lys Ile Leu Lys Ser Leu Pro Thr
Tyr 1190 1195 1200 Asn Arg Thr Ala Val His Phe Lys Asp Tyr Lys Asp
Asn Lys Leu 1205 1210 1215 Glu Lys Thr Ile Asp Tyr Arg Ile Leu Leu
Pro Leu Cys Lys Asn 1220 1225 1230 Ala Val Glu Lys Lys Glu Pro Ile
Lys Leu Ser Leu Glu Val Gly 1235 1240 1245 Asn Gln Ser Arg Thr Phe
Ala Thr Met Leu Ser Ser Glu Ile Leu 1250 1255 1260 Lys Thr Tyr Gly
Lys Asp Ala Leu Asp Glu Asp Ser Ile His Ile 1265 1270 1275 Lys Ala
Ile Gly Asn Ala Gly Asn Ser Phe Gly Ala Phe Leu Leu 1280 1285 1290
Lys Gly Ile Lys Leu Glu Ile Ile Gly Asp Ser Asn Asp Tyr Leu 1295
1300 1305 Gly Lys Gly Leu Ser Gly Gly Lys Ile Ile Ala Lys Ile Ser
Asn 1310 1315 1320 Glu Ala Thr Phe Ser Pro Glu Glu Asn Ile Ile Ala
Gly Asn Ala 1325 1330 1335 Cys Leu Tyr Gly Ala Thr Lys Gly Glu Val
Tyr Leu Asp Gly Ile 1340 1345 1350 Ala Gly Glu Arg Phe Cys Val Arg
Asn Ser Gly Ala Leu Ala Val 1355 1360 1365 Val Leu Gly Thr Gly Val
His Gly Cys Glu Tyr Met Thr Gly Gly 1370 1375 1380 Gln Val Val Val
Leu Gly Asp Val Gly Ala Asn Phe Ala Ala Gly 1385 1390 1395 Met Ser
Gly Gly Val Val Tyr Ile Phe Gly Arg His Asn Glu Ala 1400 1405 1410
His Val Asn Thr Glu Leu Val Asp Ile Lys Asp Leu Asn Ala Lys 1415
1420 1425 Asp Glu Lys Glu Leu Lys Ala Val Ile Glu Lys His Ile Thr
Tyr 1430 1435 1440 Thr Asp Ser Lys Lys Ala Lys Asp Ile Leu Glu Lys
Phe Asp Lys 1445 1450 1455 Lys Asp Phe Phe Lys Val Met Pro Arg Asp
Tyr Glu Lys Met Leu 1460 1465 1470 Lys Met Leu Asp Leu Cys Lys Asn
Glu Lys Asp Pro Asn Leu Ala 1475 1480 1485 Ala Phe Leu Lys Ile Thr
Gln Lys 1490 1495 301517PRTCampylobacter jejuni 30Met Ser Lys Phe
Lys Val Ile Glu Ile Lys Glu Asp Ala Arg Pro Arg 1 5 10 15 Asp Phe
Glu Ala Phe Gln Leu Arg Leu Ala Ser Pro Glu Lys Ile Lys 20 25 30
Ser Trp Ser Tyr Gly Glu Val Lys Lys Pro Glu Thr Ile Asn Tyr Arg 35
40 45 Thr Leu Lys Pro Glu Arg Asp Gly Leu Phe Cys Ala Lys Ile Phe
Gly 50 55 60 Pro Ile Arg Asp Tyr Glu Cys Leu Cys Gly Lys Tyr Lys
Lys Met Arg 65 70 75 80 Phe Lys Gly Val Lys Cys Glu Lys Cys Gly Val
Glu Val Ala Asn Ser 85 90 95 Lys Val Arg Arg Ser Arg Met Gly His
Ile Glu Leu Val Thr Pro Val 100 105 110 Ala His Ile Trp Tyr Val Asn
Ser Leu Pro Ser Arg Ile Gly Thr Leu 115 120 125 Leu Gly Val Lys Met
Lys Asp Leu Glu Arg Val Leu Tyr Tyr Glu Ala 130 135 140 Tyr Ile Val
Glu Asn Pro Gly Asp Ala Phe Tyr Asp Asn Glu Ser Thr 145 150 155 160
Lys Lys Val Glu Tyr Cys Asp Val Leu Asn Glu Glu Gln Tyr Gln Asn 165
170 175 Leu Met Gln Arg Tyr Glu Asn Ser Gly Phe Lys Ala Arg Met Gly
Gly 180 185 190 Glu Val Val Arg Asp Leu Leu Ala Asn Leu Asp Leu Val
Ala Leu Leu 195 200 205 Asn Gln Leu Lys Glu Glu Met Gly Ala Thr Asn
Ser Glu Ala Lys Lys 210 215 220 Lys Thr Ile Ile Lys Arg Leu Lys Val
Val Glu Asn Phe Leu Asn Ser 225 230 235 240 Asn Leu Asn Ala Asn Thr
Asp Ser Asp Glu Ala Val Pro Asn Arg Pro 245 250 255 Glu Trp Met Met
Ile Thr Asn Leu Pro Val Leu Pro Pro Asp Leu Arg 260 265 270 Pro Leu
Val Ala Leu Asp Gly Gly Lys Phe Ala Val Ser Asp Val Asn 275 280 285
Asp Leu Tyr Arg Arg Val Ile Asn Arg Asn Thr Arg Leu Lys Lys Leu 290
295 300 Met Glu Leu Asp Ala Pro Glu Ile Ile Ile Arg Asn Glu Lys Arg
Met 305 310 315 320 Leu Gln Glu Ala Val Asp Ala Leu Phe Asp Asn Gly
Arg Arg Ala Asn 325 330 335 Ala Val Lys Gly Ala Asn Lys Arg Pro Leu
Lys Ser Leu Ser Glu Ile 340 345 350 Ile Lys Gly Lys Gln Gly Arg Phe
Arg Gln Asn Leu Leu Gly Lys Arg 355 360 365 Val Asp Phe Ser Gly Arg
Ser Val Ile Val Val Gly Pro Lys Leu Arg 370 375 380 Met Asp Gln Cys
Gly Leu Pro Lys Lys Met Ala Leu Glu Leu Phe Lys 385 390 395 400 Pro
His Leu Leu Ala Lys Leu Glu Glu Lys Gly Tyr Ala Thr Thr Val 405 410
415 Lys Gln Ala Lys Lys Met Ile Glu Asn Lys Thr Asn Glu Val Trp Glu
420 425 430 Cys Leu Glu Glu Val Val Lys Gly His Pro Val Met Leu Asn
Arg Ala 435 440 445 Pro Thr Leu His Lys Leu Ser Ile Gln Ala Phe His
Pro Val Leu Val 450 455 460 Glu Gly Lys Ala Ile Gln Leu His Pro Leu
Val Cys Ala Ala Phe Asn 465 470 475 480 Ala Asp Phe Asp Gly Asp Gln
Met Ala Val His Val Pro Leu Ser Gln 485 490 495 Glu Ala Ile Ala Glu
Cys Lys Val Leu Met Leu Ser Ser Met Asn Ile 500 505 510 Leu Leu Pro
Ala Ser Gly Lys Ser Val Thr Val Pro Ser Gln Asp Met 515 520 525 Val
Leu Gly Ile Tyr Tyr Leu Ser Leu Glu Lys Ala Gly Ala Lys Gly 530 535
540 Ser His Lys Ile Cys Thr Gly Ile Asp Glu Val Met Met Ala Leu Glu
545 550 555 560 Ser Lys Cys Leu Asp Ile His Ala Ser Ile Gln Thr Met
Val Asp Gly 565 570 575 Arg Lys Ile Thr Thr Thr Ala Gly Arg Leu Ile
Val Lys Ser Ile Leu 580 585 590 Pro Asp Phe Val Pro Glu Asn Ser Trp
Asn Lys Val Leu Lys Lys Lys 595 600 605 Asp Ile Ala Ala Leu Val Asp
Tyr Val Tyr Lys Gln Gly Gly Leu Glu 610 615 620 Ile Thr Ala Ser Phe
Leu Asp Arg Leu Lys Asn Leu Gly Phe Glu Tyr 625 630 635 640 Ala Thr
Lys Ala Gly Ile Ser Ile Ser Ile Ala Asp Ile Ile Val Pro 645 650 655
Asn Asp Lys Gln Lys Ala Ile Asp Glu Ala Lys Lys Gln Val Arg Glu 660
665 670 Ile Gln Asn Ser Tyr Asn Leu Gly Leu Ile Thr Ser Gly Glu Arg
Tyr 675 680 685 Asn Lys Ile Ile Asp Ile Trp Lys Ser Thr Asn Asn Val
Leu Ser Lys 690 695 700 Glu Met Met Lys Leu Val Glu Lys Asp Lys Glu
Gly Phe Asn Ser Ile 705 710 715 720 Tyr Met Met Ala Asp Ser Gly Ala
Arg Gly Ser Ala Ala Gln Ile Ser 725 730 735 Gln Leu Ala Ala Met Arg
Gly Leu Met Thr Lys Pro Asp Gly Ser Ile 740 745 750 Ile Glu Thr Pro
Ile Ile Ser Asn Phe Arg Glu Gly Leu Asn Val Leu 755 760 765 Glu Tyr
Phe Ile Ser Thr His Gly Ala Arg Lys Gly Leu Ala Asp Thr 770 775 780
Ala Leu Lys Thr Ala Asn Ala Gly Tyr Leu Thr Arg Lys Leu Ile Asp 785
790 795 800 Val Ala Gln Asn Val Lys Ile Thr Ile Glu Asp Cys Gly Thr
His Glu 805 810 815 Gly Val Glu Ile Asn Glu Ile Thr Ala Asp Ser Ser
Ile Ile Glu Thr 820 825 830 Leu Glu Glu Arg Ile Leu Gly Arg Val Leu
Ala Glu Asp Val Ile Asp 835 840 845 Pro Ile Thr Asn Ser Val Leu Phe
Ala Glu Gly Thr Leu Met Asp Glu 850 855 860 Glu Lys Ala Lys Ile Leu
Gly Glu Ser Gly Ile Lys Ser Val Asn Ile 865 870 875 880 Arg Thr Pro
Ile Thr Cys Lys Ala Lys Lys Gly Ile Cys Ala Lys Cys 885 890 895 Tyr
Gly Ile Asn Leu Gly Glu Gly Lys Leu Val Lys Pro Gly Glu Ala 900 905
910 Val Gly Ile Ile Ser Ala Gln Ser Ile Gly Glu Pro Gly Thr Gln Leu
915 920 925 Thr Leu Arg Thr Phe His Ser Gly Gly Thr Ala Ser Thr Asp
Leu Gln 930 935 940 Asp Arg Gln Val Ser Ala Gln Lys Glu Gly Phe Ile
Arg Phe Tyr Asn 945 950 955 960 Leu Lys Thr Tyr Lys Asn Lys Glu Gly
Lys Asn Ile Val Ala Asn Arg 965 970 975 Arg Asn Ala Ala Val Leu Leu
Val Glu Pro Lys Ile Lys Thr Pro Phe 980 985 990 Lys Gly Val Ile Asn
Ile Glu Asn Ile His Glu Asp Val Ile Val Ser 995 1000 1005 Ile Lys
Asp Lys Lys Gln Glu Val Lys Tyr Ile Leu Arg Lys Tyr 1010 1015 1020
Asp Leu Ala Lys Pro Asn Glu Leu Ala Gly Val Ser Gly Ser Ile 1025
1030 1035 Asp Gly Lys Leu Tyr Leu Pro Tyr Gln Ser Gly Met Gln Val
Glu 1040 1045 1050 Glu Asn Glu Ser Ile Val Glu Val Ile Lys Glu Gly
Trp Asn Val 1055 1060 1065 Pro Asn Arg Ile Pro Phe Ala Ser Glu Ile
Leu Val Glu Asp Gly 1070 1075 1080 Glu Pro Val Val Gln Asn Ile Lys
Ala Gly Glu Lys Gly Thr Leu 1085 1090 1095 Lys Phe Tyr Ile Leu Lys
Gly Asp Gly Leu Asp Arg Val Lys Asn 1100 1105 1110 Val Lys Lys Gly
Asp Ile Val Lys Glu Lys Gly Phe Phe Val Val 1115 1120 1125 Ile Ala
Asp Glu Asn Asp Arg Glu Ala Lys Arg His Tyr Ile Pro 1130 1135 1140
Arg Glu Ser Lys Ile Glu Phe Asn Asp Ser Glu Lys Ile Asp Asp 1145
1150 1155 Ala Asn Thr Ile Ile Ala Ser Ala Pro Lys Lys Glu Arg Lys
Val 1160 1165 1170 Ile Ala Glu Trp Asp Ala Tyr Asn Asn Thr Ile Ile
Ala Glu Ile 1175 1180 1185 Asp Gly Val Val Ser Phe Glu Asp Ile Glu
Ala Gly Tyr Ser Ala 1190 1195 1200
Asp Glu Gln Ile Asp Glu Ala Thr Gly Lys Arg Ser Leu Val Ile 1205
1210 1215 Asn Glu Tyr Leu Pro Ser Gly Val Arg Pro Thr Leu Val Ile
Ala 1220 1225 1230 Gly Lys Gly Asp Lys Ala Val Arg Tyr His Leu Glu
Pro Lys Thr 1235 1240 1245 Val Ile Phe Val His Asp Gly Asp Lys Ile
Ala Gln Ala Asp Ile 1250 1255 1260 Leu Ala Lys Thr Pro Lys Ala Ala
Ala Lys Ser Lys Asp Ile Thr 1265 1270 1275 Gly Gly Leu Pro Arg Val
Ser Glu Leu Phe Glu Ala Arg Lys Pro 1280 1285 1290 Lys Asn Ala Ala
Val Ile Ala Glu Ile Asp Gly Val Val Arg Phe 1295 1300 1305 Asp Lys
Pro Leu Arg Ser Lys Glu Arg Ile Ile Ile Gln Ala Glu 1310 1315 1320
Asp Gly Thr Ser Ala Glu Tyr Leu Ile Asp Lys Ser Lys His Ile 1325
1330 1335 Gln Val Arg Asp Gly Glu Phe Ile His Ala Gly Glu Lys Leu
Thr 1340 1345 1350 Asp Gly Val Val Ser Ser His Asp Val Leu Lys Ile
Leu Gly Glu 1355 1360 1365 Lys Ala Leu His Tyr Tyr Leu Ile Ser Glu
Ile Gln Gln Val Tyr 1370 1375 1380 Arg Gly Gln Gly Val Val Ile Ser
Asp Lys His Ile Glu Val Ile 1385 1390 1395 Val Ser Gln Met Leu Arg
Gln Val Lys Val Val Asp Ser Gly His 1400 1405 1410 Thr Lys Phe Ile
Glu Gly Asp Leu Val Ser Arg Arg Lys Phe Arg 1415 1420 1425 Glu Glu
Asn Glu Arg Ile Ile Arg Met Gly Gly Glu Pro Ala Ile 1430 1435 1440
Ala Glu Pro Val Leu Leu Gly Val Thr Arg Ala Ala Ile Gly Ser 1445
1450 1455 Asp Ser Val Ile Ser Ala Ala Ser Phe Gln Glu Thr Thr Lys
Val 1460 1465 1470 Leu Thr Glu Ala Ser Ile Ala Gly Lys Phe Asp Tyr
Leu Glu Asp 1475 1480 1485 Leu Lys Glu Asn Val Ile Leu Gly Arg Met
Ile Pro Val Gly Thr 1490 1495 1500 Gly Leu Tyr Gly Glu Gln Asn Leu
Lys Leu Lys Glu Gln Glu 1505 1510 1515 31156DNACampylobacter jejuni
31atggcttatg aagatgaaga agatttaaat tacgatgatt atgaaaacga agatgaagaa
60tatccacaaa atcaccataa aaattataat tacgatgatg atgattatga atacgatgat
120gataacaatg atgatgattt ttatgagatg gattaa 15632198DNACampylobacter
jejuni 32atgatcaatc ctatacaaca aagttatgtg gcaaataccg cattaaatac
aaatagaata 60gataaagaaa ctaaaacaaa cgatactcaa aaaacggaaa atgataaagc
gagtaaaatc 120gcagagcaga ttaaaaacgg tacttataaa atcgatacaa
aagctacagc tgctgcgatt 180gctgactctt taatctaa
19833351DNACampylobacter jejuni 33atgaaaaaaa tacttattct acttacacta
tgtgcttttg cttttggtgc aagtgaatgt 60gatagaaaaa tcgatcgtat caataaagaa
atcagttttt ctaaagcgca taatgataca 120gctagaactt taagcttaga
gcttgcttta aaacaagtac aaaatgattg tgctaaagat 180cctatgtttt
atgataaaaa gttagaagct aaaaaactta aagaacaaga agtggaaaaa
240atcgaaaaag aacttgatgc tttaaaagaa caaaaagatt atatgagcaa
ggctgagtat 300aaagctaaaa aagaagcttt aaaagaacaa aaagagaaaa
tcaaaaaata a 35134417DNACampylobacter jejuni 34atgagttttg
gtgaaattat agttatttta gttgtagcga ttttagtctt aggacctgat 60aaacttccag
aagctatagt acaaatagca aaaattctaa aggctgtaaa acgcaacata
120gatgatgcaa aatcaagcat agaaaaagaa atacgcatca atgacttaaa
agaagaagct 180aagaaataca aagatgaatt ttcaagcact aatgaaaata
tacgcaaaaa actcagcttt 240gaagaatttg atgaccttaa gagagatatt
ttagataaaa caaaggtaga tttaaccttt 300gatagcagag atgataaagt
aaaaaataac cttagcggac aaaatttaaa tacagaagaa 360aaaccaaatc
ttagcaaatt agaaacacaa gataaaaacg gaaaaataaa tgtttga
41735426DNACampylobacter jejuni 35atgcaaaaag ctaaaatttt aattgcctta
agtttttttt tattagtttt atctgcctgt 60tctaatgatg aaaaaaatat ttccaagact
caaaatacag atcaagaagt agtccaaata 120gaacaaaacg atgaaaaaac
agaattaagt gactccaacc tgcctttacc tgtagatgat 180gaagcacaaa
gttcaaatga tgaacatgaa gtcaatccta gtattatcaa ctctttatat
240aaacaaaaat gcgccacttg tcatggagaa aaaggggaat taaaacctaa
aaacagcacg 300gccattaaaa ccttgagcaa taaaattttt atacaaaaaa
taaaaacgat aaaagataaa 360aaccatagtt ttttaagtga tgaacaaatt
caaaatttag ctgattttat aaacaaagga 420aaataa 42636435DNACampylobacter
jejuni 36atgcgaagac taagtatttt acttgcaatt ttaatagtta ttaatataac
agcttgtgat 60agtaaaacag aaaattacta caaaaatctt cctagcgaag caaaagaaaa
agcgaaagaa 120tgcaaagaaa gcggaactct tagcgaagat tgtattaatg
cattaaaagt tggtgttaaa 180ccaacaaatg aagagggtaa atacagtcca
aacacaccga aaaaatctga taatcaaata 240ttagaagctt taaaacaaaa
tgatttaaaa aaagaaaaaa ccacaaaaga tattaaccaa 300agttcagaaa
ataatgaaag tattataata ccacctatag cagaaacacc ttctgaaatt
360tatccttcca aaacaacaga aaacaatcaa agttctatct ttagcgatga
tgttaatatg 420acacaggaaa aatga 43537525DNACampylobacter jejuni
37atgatgaaga agaaattggt tttattagga agtgctgcag tggtattttt tgccgcttgt
60gcaatgaata gtggggtaag ttcagaacaa attggactta gaaaagcaag tttagaaaat
120gaaaataaag taaatttagt ggaggcaaat ttcacaactt tacagcctgg
ggaatctact 180cgttttgagc gttcttatga aaatgcacca ccattaattc
cgcatgctat tgaagatttg 240ttacctataa ctaaagataa caatatgtgc
ttaagctgcc atgataaggc tatagcagca 300gatgctggtg caactccact
tcctgctagt cattattatg attttagaca caataaaacc 360acaggagata
tgattagcga tagtcgtttt aattgcactc agtgtcatgt tccacaaagt
420gatgcaaaac ctttagtggg aaatagcttt aaacctgaat ttaaaaatga
acaattaaaa 480agtcgttcaa atttaattga tgtgattaat gagggtgtaa agtaa
52538606DNACampylobacter jejuni 38atgaaaaaaa tcaaaaaaat cattcaaatt
ggtatgatag gtggtttagc agctgttgct 60ggaggtgctt tagcaggttg tggaagtaat
aatgacaatg cagatacttt aaatcaagcg 120gctaatgctc aaggagcttt
tgtaattatc gaagaaacag ctccagggca gtataaaatc 180aaagatcaat
atccaagtga tgaaacaagg gtagttttaa aagatcttaa tggtacagaa
240agaattttat ccaaagaaga aatggatgct ttgattaaag aagaagcagc
taaaattgat 300aatggaactt caaatttaac taaagacaat ggacaaatca
gtagtggggg attgagttta 360ggggaaactt tactcgcaag tgctgcaggt
gctattttag gaagttggat aggttcaaaa 420cttttcaata atcaaaattt
tgccaaccaa caacgcggtg ctttttcaaa tcaaagtgct 480tatcaaagga
gtgttaatag ttttaataaa gcaggcacaa caagctctgc ttcaagtgct
540aaaaaatcag gtttttttgg tggaggctct aaagccacaa gttctagttc
ttcttttggc 600tcttaa 60639633DNACampylobacter jejuni 39atgacaaaat
ttttaagcat ttgtagttta attgcgatgt tgttaagtgg ttgtggaagt 60gattttcctg
gacaacctag cgatgtggct agagttcagc aaaacaaata tccaaatgga
120aatttaaaaa aagaaattcc ctataataaa gattcaagaa tacatgggtt
aaaaagagct 180ttttatgaca atggtcagct aagagctgaa gaaaattata
aaaatggaaa aaaagatgga 240attagcaggg aatattctag aaatgggcaa
ttacttgagg aggtgcattt taaagataat 300cgcggatatg gtgattttgc
aagctattat gagaatggaa atatgagagc aaagggaaaa 360ctacttggct
ataatgaaga tggtatgcca gaatttgaag gtaattacaa ggaatattat
420gaaaatggaa ctttaatgtg tgattataat tttgataata aaggtaaatt
tgatggagta 480caaaagcgtt atgatgaaaa tggcgcctta gaagacgaag
aaaattataa aaatggactt 540aaaaatggag tctttagaga atataaaaaa
ggggagattg taagagaaga agaatataaa 600aatggtattt tagtagcaaa
acctaaaaat tag 63340735DNACampylobacter jejuni 40atgaaaaaaa
tatttttaag tgtttttttg gttttgagtt taaatgctca aaatcttgaa 60atagataaaa
taagaacaga tttgtattct aaaagtggag caaatgttct taaaaaagtt
120gaaatttctt tggaatttga tgggaataat ttaaaagaaa atgaaaataa
gttaattgat 180gctgtaaata cagtaatttc agggtttttt tatgaagata
tttttacaga aattggaaaa 240aataatttta aaaaaacttt agaaaaattt
ttagataaga aatataagat taaactagat 300gatatatata tcatatcttt
aagtggagtg gaaaaatttg atttagagga gtttaaacgc 360tttttagaaa
gtactgaggc taaagagaag ggtatgggta gcgaggtaaa aaaagcactt
420gaaaacttag aagttcctaa aactcaagtt cctagtgttg aaaagatccc
aactcctagt 480gttccaaatt tagaagtcaa gcaagttgaa cagcttttca
aagatccaga tgaagaaaat 540aaaaatgaca atggagaaat caatatagat
aatttaaata cacctaaaat gactccagat 600atagaagaaa aaattaaaag
agatttaatc gccaatcctc cacaaatatt caaagaaaat 660aacgcaagca
agccttatca tttgccacaa acaggctatg atataaagct tgatgagaat
720tcaacgcaaa attag 73541780DNACampylobacter jejuni 41atgaaaaatt
atggtttgag taatttaaat tcgtttttac ttgctttagc aatatatatt 60agtatagtaa
ttcttgtttt ttttagactt gtaagcgagg ttgaacctgc tatacaatat
120actgatataa aagatagttt tgtagatatt gaacttgctg aaccatcaaa
acaagttatt 180actcaaagca acactcctaa agaaatacaa aaaccaacag
agcaaattga tatagaaaag 240ctttttgctc agactacaaa taaaacagtt
aaaactgaag atattgacca aaaggcaagt 300aattttaatg agctttttgg
aaatattaaa gaaatacaag aagaaaagac tacaaaaatt 360caatcaagtg
ctaaatcagg aatttctagt gccccaaaac ctcaagcttc tgaacttgta
420aaacaactta atgatagttt acttcaagaa gaaagttcaa cgcaaggcga
aagcacaaag 480gcgcaaaaga ttggaattta tgatgaattt ttagggaaag
ttgtgcgtat tatcactcaa 540agatggactc agtattatcc aaatagtgaa
aaaatttctg ttaaggtaaa aatttttatt 600gatgaaaatg gaaaatttgg
ctatacttca gttgaaaaaa gtgggaatcc tttatatgat 660gctaaagtgg
ctgaattttt agaaagtcaa aaaggtaaat ttattactta tcctccgcaa
720aataaaaata taagcattac catgaattta agagatgaag taaaagttaa
aaatgattag 78042822DNACampylobacter jejuni 42atgaaaaaat tttctttagt
cgcagcaact ttgattgcag gtgtagtttt aaatgtaaat 60gcagctacag tagctactgt
taatggcaag agcattagcg atacagaagt aagtgaattt 120tttgccccta
tgcttagagg acaggatttt aaaactttgc cagataatca aaaaaaagct
180cttattcagc aatatattat gcaagattta attttgcaag atgctaaaaa
acaaaattta 240gaaaaagacc ctttatacac aaaagaactt gatcgtgcaa
aagatgcaat acttgttaat 300gtttatcaag agaaaatttt aaatactatt
aaaattgatg cggctaaagt taaagctttt 360tatgatcaaa ataaagacaa
atatgtaaaa cctgcaagag tgcaagcaaa acatatctta 420gtagcgacag
aaaaagaagc taaggatatt attaacgaac ttaaaggttt aaaaggtaaa
480gaactagatg ctaaatttag cgagcttgct aaagagaaat caattgatcc
aggttcaaaa 540aaccaaggtg gtgagcttgg ttggtttgat caatcaacta
tggtaaagcc ttttacagat 600gctgctttcg cgcttaaaaa tggtactatt
actacaactc cggtaaaaac gaattttggt 660tatcatgtaa tcttaaaaga
aaattcgcaa gctaaaggtc aaatcaaatt tgatgaagta 720aaacaaggta
ttgaaaacgg acttaaattt gaagaattta aaaaagttat caatcaaaaa
780ggccaagatc tcttaaatag tgctaaagtg gaatataaat aa
82243837DNACampylobacter jejuni 43atggaaaatc aaaaaaatga atttgatgat
attattttag aaaaaagtaa taaaagtgaa 60aaagtaaaaa aaattctttt acgagttatt
gctttagtta ttttgttttt agctatcatg 120atagttatga agcttattaa
tggtagtggt gatgaaaata cgcaaaatca aagtgtattg 180ccaagtgaac
ctatagcaac tcaagacaat aacaatgata cttcttttga aagtatgcca
240attacagata atacttcagc agaagatcaa tttgaggcat taagaaaaca
atttcaagat 300gaacaaaata caactcaaaa tacaacaacc tctagttcaa
ataacaatga tactacaaat 360tttgctatgc ctgatcaaga agttccagca
gaaccaacag caactacttc agcaaatacc 420actccacaag caagtactcc
taaacaagaa gtaacacaaa ctgcaaaatc taaagaagaa 480gcaaaaaaac
aaacagctgt aaaaaaagaa aaagaaagtg caaaacaaac ccctaaaaaa
540gaacaaaatg caaatgattt atttaaaaat gttgatgcta aacctgtaca
tccaagtggt 600ttagcatcgg gtatttatgt gcaaattttc tcagtaagta
atttggatca aaaatcaaaa 660gaacttgctt ctgtaaagca aaaaggttat
gattataaac tttataaaac tacagttgga 720agtaaagaaa ttaccaaggt
tttaatagga ccatttgaaa aggcagatat tgcagcagaa 780cttgctaaaa
tccgtaagga tattgcaaaa gatgcttttt cttttacttt aaaatga
837441173DNACampylobacter jejuni 44atgaaaaaga agattgtttt aatcatttta
attgcaatac ttggaagtgt tggggcttat 60tttatttttt ttaataatga tgaaaaaatc
agctatttaa ctcaaaaaat acaaaaaaaa 120gatatatctc aaaccataga
ggcagtagga aaggtatatg ccaaagatca agtcgatgtg 180ggtgctcaag
ttagtggaca aattataaaa ctttatgttg atgtgggaac tcatgtaaag
240caaggtgatt tgatcgctca aattgataaa gataaacaac aaaacgattt
agatattaca 300aaagctcagc ttgaaagtgc taaggctaat ttagagagta
aaaaagttgc ccttgagatt 360gcgaataagc aatatcaaag agagcaaaaa
ctttatgcag ctaaagcaag ttctcttgaa 420aatttagaaa ctcaaaaaaa
taattattat actttaaaag ccaatgttgc agagcttaat 480gctcaagtag
ttcagcttga aatcactctt aaaaatgcac aaaaagattt aggttataca
540accattactg ctcctatgga tggtgttgtg attaatgtag ctgtagatga
aggacaaaca 600gttaatgcta atcaaaacac tcctactata gtccgtatag
ctaatttaga cgaaatggaa 660gtaagaatgg aaatagcaga ggctgatgtg
agtaagataa aagtaggaac agagcttgat 720ttttctttgc ttaatgatcc
tcaaaaaact tatcatgcta agattgcaag tatagatcca 780gctgatactg
aagtgagtga ttctagtaca agctcaagct catcaagttc gagttcttca
840agctcaagct catcaaatgc tatttattat tacgcaaaat tttatgtagc
caataaagat 900gattttttgc gtattggtat gagtatacaa aatgaaattg
tcgtagcaag tgcaaaggct 960gttttagcag tgccaactta tgcaattaaa
agcgatccaa aaggctatta tgttgaaatt 1020ttggaaaatc aaaaagctgt
taaaaaatat gtcaaacttg gcattaaaga ttctatcaat 1080actcaaattt
tagaaggtgt aaatgaagat gaagaattga tagtaagctc aagtgctgat
1140ggtttagctc ctaaaatgaa gttgagattt taa 1173451629DNACampylobacter
jejuni 45atgaaaattt tacttttaaa tgaaaaccct gtagtttcaa gacttgtaag
ccttagtgct 60aaaaaaatgt cttatgattt tgaagaactt aatgcttata gtgaaaattt
gggtaattat 120gatgtgattg ttgtagatag tgatactcca gcacctttaa
aaattcttaa agaaaaatgc 180gataggttga tttttttagc cccgcgaaat
caaaatgtag aagatataga tgcgcaaatt 240ttacaaaaac cttttttacc
tacagatttt ttaaatttac ttaataataa agatgcgaac 300aagcatacat
ctattgattt gccaatgtta agcaatgatg aaaatcctta tgctgatata
360agcttggatt tagataattt aaatttagat gatttgcctg atgaaaattc
tttagatata 420aattcagagg gaatggaaga tttgagtttt gatgatgatg
ctcaagatga taatgcaaac 480aaaactttag aaactcaaaa tttagaacat
gaaacaatta aagaacagac tcaagaagac 540actcaaattg atttagattt
aactttagaa gatggcgaaa gtgaaaaaga agacttaagc 600caagaacata
cagctttgga tactgagcct agtttagatg agctagatga taaaaatgat
660gaagatttag aaatcaaaga agatgataaa aatgaagaaa tagaaaagca
agaattatta 720gacgattcta aaacaaatac attagaaatg caagaagagc
ttagcgaatc tcaagatgat 780aattcaaaca aaactttaga aactcaaaat
ttagaacatg acaatttaga acaagaaaca 840attaaagaac agactcaaga
agacactcaa attgatttag atttaacttt agaagatggc 900gaaagtgaaa
aagaagactt aagccaagaa catacagctt tggatactga gcctagttta
960gatgagctag atgataaaaa tgatgaagat ttagaagata ataaagaatt
acaagctaat 1020ataagcgatt ttgatgatct tcctgaggtt gaagagcaag
aaaaagaaat ggattttgat 1080gatcttcctg aggatgctga atttttaggt
caagcaaaat ataatgaaga atcagaggaa 1140aatttagagg agtttgctcc
tgttgtggaa gaagatattc aagatgaaat agatgatttt 1200gcttcaaatt
tgagtactca agatcaaatc aaagaagaac tagctcaact tgatgagctt
1260gattatggta ttgatagtga caatagtagc aaggtcttag aagattttaa
agatgaacca 1320attttagacg ataaagaatt aggaacaaat gaagaggaag
tggttgtgcc aaatttaaat 1380ataagtgatt ttgatacatt gaaagaaagt
gatatacaag aagctcttgg agaggaaatc 1440ttagaaaaaa atgaagagcc
tatagtaagt gatgtaacta aagatgataa tagcgaagag 1500atagttaatg
agcttagcca aagcatagca ggagcgatca cttcaagtat taaagatgat
1560accttaaaag ctgctcttaa gggtatgaat atgaatataa atataaacat
tagttttaaa 1620gaggattga 1629461833DNACampylobacter jejuni
46atgaaaaaaa atattttacg cttaggtatt gtcgttttgg ttttacttat agcgggagtt
60ttatggctaa ataatgatat caatcaaaaa aaagaagatg aagcaaataa aaatgccatt
120gcagcaaatg ctgatttttc tttgcttagc gatgatgatc caaattttga
aaaatggggt 180aaggtttttc cagagcaact aaaaatgtat ttaacggttg
aaaaagaaga gcctaaggct 240actgaatttg gtggtaattt agcttattca
aaattaattc gctttcctca attaaccata 300ctttgggcag gatatccttt
tagtcttgat ttcaatgaag aaagagggca tttttgggtt 360caagtagatc
aaatgaaaac agcaagaaac aacaaagatt ttcttaatgc ccatggactt
420gctgctttta aaggtcaacc tgcagcttgt atgaattgtc atagtggatg
gactccatgg 480cttataaaaa atgttgctaa aggagatttt actgctttta
actctacaaa ttattggact 540atgattaaaa atatcccagc tgttgatggt
atagtagaaa attcacctga acatgcaggc 600ccacatggtg gtaaaagaat
gggtgtaact tgtgcagatt gtcacaatcc aaatgatatg 660agtttaagac
ttactcgtcc agcagctatt aatgctttag tttctagagg atatgaaaaa
720gatccagtac aaggcgtaaa agctacaaga gaagaaatga gaactttagt
ttgctctcaa 780tgtcatgttg aatactattt taaaccaaca ggggaaaaag
taaaagtaat gggtgaaact 840attgtagatg atagttctaa aaaatggtgg
aatggaactc agaaaaatta tgatgagtat 900gagttttgga gagatggcaa
taaagttaaa gagattgaaa ccgatggtat agttttaact 960tttccttgga
gtgagtggaa aaaaggacaa ccatttagaa ttgaaatgct agatgattat
1020tatgataaag ttcgtggagt atttggagct gattttactc ataaattaac
aggagcgcaa 1080attattaaaa ttcaacatcc agaaagtgaa ctttatagtg
gcggtgtgca tgctgcaaat 1140ggagtaagtt gcgtggattg tcatatgcct
tatgttagag aaggagctaa aaaggttact 1200caacacaata tcacttctcc
tttaagagat attaactctg cttgtaagtc ttgtcataaa 1260caaagtgaag
attatttaaa agctcaagtg cttgacatac aaaacagcgt tgcacatgat
1320caaagaactg cagagtatgc aattgttagt ttgattatgg atactaaaaa
attacgcgat 1380gaactaggca atatggaaaa attccaaagc gatggaaaag
ctgatgcgaa aaaaattagc 1440gaagagctaa aagaagtttt agaacttcat
cgtaaagctc aaatgagagc ggattttgtt 1500aatgctgaaa actcaactgg
tttccataat cctcgcgaag cttcaagaat gttattgcaa 1560gctgttgata
tggcaagaat gggacaaact aaacttgtag aaattgcagc ggcaaatgga
1620attaaagatt ttaaaacttc aaatttaggt tttgaagata ttcaaaaatt
taatccagga 1680gagctttatt ataaagtaga tgttaataat cataaagctg
gagagcgtta ctatgcagat 1740gaaaaagatg ttaatggcaa tcctccaaaa
gaacttttag agcatgataa agagcttgct 1800ccttataatt atcaagtgat
tgataaaaaa taa 1833471980DNACampylobacter jejuni 47atgaaaagcg
taaaattgaa ggtttcgctg attgcaaatt taatcgcagt agtgtgtttg 60ataattttag
gtgttgtaac atttatattt gtaaagcaag caatttttca tgaagttgtg
120aatgctgaaa taaattatgt taaaacggct aaaaattcta tagagtcttt
taaggcaaga 180aattctttag ctcttgaaag tttagctaaa agtattttaa
agcatcctat agaacagtta 240gatagtcaag atgctttaat gcattatgtt
ggaaaagatt taaagaattt tagagatgct 300ggaagattct tagcagttta
tattgctcaa ccaaatggcg aacttgttgt aagcgatcca 360gactctgatg
ctaaaaattt agattttgga
acttatggaa aagctgataa ttatgatgct 420agaacaagag agtattatat
agaagcagtt aaaacaaata aactttatat taccccatct 480tatattgatg
taactacaaa tttaccttgc tttacatatt ctattccgct ttataaagat
540ggtaaattta taggggtttt ggctgtagat attcttgcgg cagatttgca
agctgaattt 600gaaaatttac caggtagaac ttttgtattt gatgaagaaa
ataaagtatt tgtttctaca 660gacaaagctc ttttacaaaa aggttatgat
attagtgcaa ttgcaaatct tgctaaaact 720aaagaggatc ttgaaccttt
tgagtatact agaccaaaag atggtaatga aagatttgct 780gtatgcacaa
aggtttctgg aatttatact gcttgcgttg gagagccaat agaacaaata
840gaagctccag tttataaaat tgcatttata caaactgcga ttgttatttt
tacaagtatt 900attagcgtca tcctccttta tttcatcgta tcaaaatacc
tctccccact tgcagctatc 960caaacaggtt taacttcatt ctttgatttt
atcaactata aaacaaaaaa tgtttccact 1020atagaagtaa aaagcaatga
tgaatttgga caaatctcaa atgctatcaa tgaaaacatt 1080cttgctacta
aaagaggctt agaacaagac aatcaagccg ttaaagaatc agttcaaacc
1140gtatcagttg tagaaggtgg taatttaaca gcaagaatta ctgctaatcc
aagaaaccca 1200cagcttattg aacttaaaaa tgttctaaat aaacttcttg
atgttttaca agctagagta 1260ggttctgata tgaatgctat tcataaaatt
tttgaagaat acaaaagctt agactttaga 1320aataaattag aaaatgctag
cggtagtgta gaattaacta ctaatgcttt aggtgatgaa 1380atagttaaaa
tgctaaaaca aagttcagac tttgctaatg ctttagctaa tgaaagtgga
1440aaattacaaa ctgctgttca aagcttaacc acttcttcaa attctcaagc
tcaatcttta 1500gaagaaactg cagcagcttt agaagagatc acttcttcta
tgcaaaatgt ttcagttaaa 1560actagtgatg ttatcactca atctgaagag
attaaaaatg ttacaggtat tataggtgat 1620attgcagatc aaatcaatct
tttagcttta aatgcagcta ttgaagcagc tcgtgctgga 1680gaacatggta
gaggctttgc agtggtagct gatgaagtta gaaagttagc tgaaagaact
1740caaaagtctt tatcagaaat tgaagctaat actaatttac ttgttcaatc
tatcaatgat 1800atggcagaaa gtattaaaga acaaactgca ggtatcactc
aaatcaatga tagcgtagct 1860caaattgatc aaactactaa agataatgtt
gaaattgcta atgaatcagc tattatttct 1920agtacagtaa gtgatatagc
taataatatc ttagaagatg ttaagaagaa gaggttttaa
1980481998DNACampylobacter jejuni 48atgcaatcaa taaattcagg
caaatccgtt ggaatttcag ctaagcttac gctatgggtt 60ggaattttag ttgtattaat
tttagcaatc acaagtgcta ttagttactt tgattcgaga 120aacaatacat
atgaattgct aaaagacact cagttaaaaa ctatgcaaga tgtggatgct
180ttctttaaaa gctatgctat gtcaaaaaga aatggtattc aaatactagc
caatgagcta 240acaaatcgtc ctgatatgag cgatgaagag ctaatcaatc
ttatcaaagt aattaaaaaa 300gttaatgact acgatctagt ttatgtagga
tttgataata caggaaaaaa ttatcaatct 360gatgatcaaa ttttagatct
atcaaaaggt tatgatacta aaaatcgtcc ttggtataaa 420gctgccaaag
aagcaaaaaa gcttatagta acagaacctt ataaatccgc cgctagcgga
480gaggttggtt taacttacgc tgctccattt tatgatagaa atggaaattt
tagaggtgtt 540gtaggtggag attatgatct agcaaatttt tcaaccaatg
ttttaactgt aggaaaatca 600gacaatacct ttactgaagt acttgattca
gaaggaacaa tactttttaa tgatgaagtt 660gctaaaatac taacaaaaac
agaattaagt atcaatatcg ccaatgcaat caaagcaaat 720cctgctctta
ttgatccaag aaaccaagat actttattta ccgctaaaga tcaccaaggc
780gtagattatg cgattatgtg taattctgct tttaatcctt tatttagaat
ttgtacaata 840acagaaaaca aagtttatac cgaagctgtt aattctattt
taatgaaaca agttatagtt 900ggtattatag ctataatcat agctttaatc
ttgattagat ttttaatcag cagaagtctc 960tccccacttg cagctatcca
aacaggttta acttcattct ttgattttat caactataaa 1020acaaaaaatg
tttccactat agaagtaaaa agcaatgatg aatttggaca aatctcaaat
1080gctatcaatg aaaacattct tgctactaaa agaggcttag aacaagacaa
tcaagccgtt 1140aaagaatcag ttcaaaccgt atcagttgta gaaggtggta
atttaacagc aagaattact 1200gctaatccaa gaaacccaca gcttattgaa
cttaaaaatg ttctaaataa acttcttgat 1260gttttacaag ctagagtagg
ttctgatatg aatgctattc ataaaatttt tgaagaatac 1320aaaagcttag
actttagaaa taaattagaa aatgctagcg gtagtgtaga attaactact
1380aatgctttag gtgatgaaat agttaaaatg ctaaaacaaa gttcagactt
tgctaatgct 1440ttagctaatg aaagtggaaa attacaaact gctgttcaaa
gcttaaccac ttcttcaaat 1500tctcaagctc aatctttaga agaaactgca
gcagctttag aagagatcac ttcttctatg 1560caaaatgttt cagttaaaac
tagtgatgtt atcactcaat ctgaagagat taaaaatgtt 1620acaggtatta
taggtgatat tgcagatcaa atcaatcttt tagctttaaa tgcagctatt
1680gaagcagctc gtgctggaga acatggtaga ggctttgcag tggtagctga
tgaagttaga 1740aagttagctg aaagaactca aaagtcttta tcagaaattg
aagctaatac taatttactt 1800gttcaatcta tcaatgatat ggcagaaagt
attaaagaac aaactgcagg tatcactcaa 1860atcaatgata gcgtagctca
aattgatcaa actactaaag ataatgttga aattgctaat 1920gaatcagcta
ttatttctag tacagtaagt gatatagcta ataatatctt agaagatgtt
1980aagaagaaga ggttttaa 1998492253DNACampylobacter jejuni
49atgagaatta caaataaact taacttcaca aatagtgtga ataattctat gggcggtcaa
60agtgctttat atcagatatc tcaacaactt gcttcaggtt tgaaaataca aaattcatat
120gaagatgcaa gcacttatat agataatacg cgtcttgaat atgaaattaa
aacgttagaa 180caagtaaaag aatcaacaag tagagctcaa gaaatgactc
aaaatagtat gaaagcttta 240caagatatgg ttaaacttct tgaagatttt
aaagttaaag taacccaagc tgcaagcgat 300agcaattctc aaacctcaag
agaagctata gcaaaagaac tagaacgtat aaaagaaagc 360atagttcagc
ttgcaaatac cagtgttaat ggtcagtatc tttttgcggg ttctcaagtt
420gcaaacaaac cttttgattc taatggaaat tattatggag ataaaaataa
tattaatgta 480gtaactggag ctggaactga aagcccatac aatataccag
gttgggattt attttttaaa 540gcagatggag actataaaaa acaaataagc
actaatgtta gttttacaga taatcgttgg 600gatttaaata aagaccctga
taaaactaaa tatctcacag gagattctaa atggcaacaa 660cttatcggac
aaagttatgt aaaagataat agcttagatg ctgacaaaga ctttgagtat
720gatgatagca aactagattt tcctccaaca actctttatg ttcaaggtac
aagacccgat 780ggaacaagtt ttaaaagtgc tgtactcgtc aaacccgaag
atactttaga agatgtaatg 840gaaaatattg gagctcttta tggtaatact
ccaaataata aagtagtaga agtaagtatg 900aatgatagtg gtcaaattca
aattacagat ctaaagcaag gtaataataa actcgatttt 960catgctgtag
ctttcacacc acaagctgat gataaaactg aattaaataa tattatccaa
1020gcagcacagg atgaaggcat tacaatggaa gatgttacaa acagggttat
gactgctgca 1080ctaggaaatc ccaataatgg agatattaca aatttaaata
atcctgtaac cattcaaatt 1140aacggacaaa actttgaaat tgatttaaaa
caaactgatt ttatcaaaag taaaatgaca 1200gatacagatg gaaatgctgc
taatggagct gattacgata atgtgtattt tgaaaaaaat 1260ggcaatactg
tttatggtaa tgtttctcaa gttatcaaag gaagcaatgc ttatgccact
1320gattcaacca aacttagcga ggtaatggca ggagatagcc taaatggtac
tactttaaat 1380ttaaaagtca attccaaagg tggaaattct tacgatgtta
ctataaattt acaaacttca 1440actgtaagct atcctgatcc taataatcca
ggtcaaacca taagctttcc tattatgcat 1500actaatcctg caactggaaa
tagtggggtt gttacaggat caaatgatat tacttatggt 1560caaattaatg
atattatagg tatgtttgct gcagataaaa ttcctacaac aaccatacaa
1620gccaataatg gtcaaattaa taatgcagat tatacccaaa tacaacaact
catgaaagat 1680tcacaagcta ctgttgatgt aagtatggat tataaaggtc
gtattagtgt tactgataaa 1740ctttcatcag gaaccaatat agaaatttct
cttagtgatt ctcaaagtgg ccaatttcca 1800gcacctcctt ttaccacaac
ttctactgta caaaatggtc caaattttag ttttagcgcg 1860aataattctt
taactataga tgagccaaat gtggatatta ttaaagattt agattcaatg
1920attgatgctg ttttaaaagg caatatgaga gcagactccg aaagtgaaaa
ccctagaaat 1980acaggtatgc aaggagcttt agaaagactt gatcatttag
cagatcatgt tagcaagctt 2040aatacaacta tgggagcata tcataatact
atcgaaggtg ttaatacacg cacatcattt 2100ttaagcgtta atgtccaaag
tataaaatca aatgtgattg atgtagatta tggtgaggca 2160atgatgaatt
taatgcaagt tcaacttgca tatcaagctt ctcttaaagc tagtacaaca
2220atttctcaac ttagcttatt aaattatatg taa 2253502598DNACampylobacter
jejuni 50atgatgagat cactttggtc tggcgtaagc ggactacaag cacatcaagt
tgcgatggat 60gttgaaggta ataacatttc aaatgttaat accactggtt ttaaatattc
tcgtgcagat 120tttgggacta tgtttagcca aactgtgaaa atcgctacag
ctccaactga tggaagaggc 180ggatctaatc cacttcaaat cggtcttggc
gtttcagtaa gttctacaac tagaattcat 240tctcaaggtt cagttcaaac
cacagataaa aacactgacg ttgctataaa tggcgatggt 300ttttttatgg
taagcgatga tggtggtctt acaaactatc ttacaaggag cggggatttt
360aaactagatg cttatggaaa ttttgttaat aatgcaggtt ttgttgtcca
agggtggaat 420atcaactggg atgatcaaac tatagatagt tcaagaactc
cacaaaatat ttttatcgat 480ccaggtatgc atatccctgc agcaaaatct
actgaagttg ctatcaaagc gaatttaaat 540agtggtttaa atataggaac
ttcaagtaga aatctttatg cacttgattc tgttcatgga 600tggaatacta
aaacccaaag agcagaagat gaaaatgata caggaactac tcagttttat
660acgacttcta agaattctgt agaagtgaca gaaaagggtg tggatgcggg
atcacttttt 720aacgcgaaag gacaaggact taatcttaga gatggacaag
gaatttgggt atcttatgca 780gatgcaacat attctaccaa taaagtagga
gtaaatgctt ttgatccaaa tttacagcaa 840aatcaaactg ctgctttttg
gggaacagct aatcaaaaag tgaatttaga tataacttta 900aatggggtta
gaattcaaaa tgctgatatt caaagtattg atgatgctat tgcttatatc
960aataccttta ctgcaccaac ggatacaagg gatggaacag gtgtaaaagc
ggttaaaaat 1020aaggatggta gtggaattga ttttgtcaat gataatgccg
atggtactac agataatatg 1080aaaaatatca atcttgtggt tgccaatacc
aatacagcag gtgagctttg gaatgctgta 1140tggaataaca acaatcaaac
atttacattt aataataatg gtaatggaca ggctggaaca 1200ccgactatta
ataaaaatgg ttcttctttg tggacagcta caaatattac atttacacca
1260caacctcctc aagcagctac gaatgttcag cttactggtg gactaaatgc
acaaataata 1320acagcacata aatatattta tagttcaaac cctgtggata
taggtcctat gtataatcct 1380gacggtggac cagcattcca gcctggtgct
aatgcaacta caagaccaac tgaaccaggt 1440tcagcagctt attgggatgc
tgttaatggt ggacttttaa atactaatgt aagaactttt 1500agaaccacag
aagatttaag agaactttta caaagggatg ctagatatgg ggttgattat
1560gatggaagtg gaacttttgc tgcagctgat attaatcaaa atataaaagt
agtagtaacg 1620gcagatggac attttgctat ttccaatgct aatgaacaat
caactgttcc accaaatgct 1680attaatggtg taggaaatgc cactacaaca
gatccaaaaa atatgagttt taatataaca 1740gcttatagta acaaacaagg
aactgtaagt actaatgatg ctttcactgc tatttttaaa 1800gctttcgatg
gtcctttggt tataggaaat cagatcaaag aaagcgaaca acttaagctt
1860tctgcttttt cggcggggct tgaaatttat gattctttag gttcaaaaca
cactttagaa 1920gtgcagtttg ttaagcaaag taccactcaa gatgggggta
atgaatggca aatgatcatc 1980cgtgtacctg aacctgcaga gattaacact
acaggcgaag gaccaaacaa tatcatcgta 2040ggaacagcta gatttaacaa
tgacggctct ttagctagtt atacaccaag aacgataaat 2100ttctcaccaa
acaatggtgc cgcaccaaat caacaaatca aactttcctt tggaacaagt
2160ggaagcaatg acggccttgt aagctcaaat tctgcttcaa ctctaacagg
acaggcaact 2220gatggttata cttcaggtaa cttaaaacct gatgctatcc
gtgtggatga taaaggtaat 2280atcttaggtg aatttactaa tggcaaaacc
tttgctgtag caaaaatcgc aatggcttca 2340gtggcaaata actcaggtct
tgaggaaatt ggtggaaatc tttttaaagt tactgcaaat 2400agtggtaata
tcgtggtagg tgaagcagga acaggaggtc gtggtgagat gaaaacctca
2460gctcttgaaa tgtcaaatgt ggatttaagt cgttctttaa cagagcttat
tatcattcaa 2520agaggttatc aagcaaactc aaaaaccatt tcaacgagtg
atcaaatgct ccaaactcta 2580atccagctta aacaataa
2598512616DNACampylobacter jejuni 51atggcaaaga ttagaattca
tgaaatcgca aaagaattag gttatgatag taaggaaatt 60attgaaaagg caaatgaatt
aggacttgga attaaaacag catcaaatgc tgtagaacct 120gagattgcgg
cagctattta tgagtatata caaacaagag aaattccaga agcttttaag
180aaaaatatca aaactcctac agcaaaaaag cctaaaaaag aaaatataaa
agaacaagaa 240aagctaaatg aatctgaaaa aaaagaacct aaaaaagaag
aaaagcttaa acaagaagtt 300aaaaaagaag aattaaaaat tgaaaaagaa
aatgcaaaag aggaagaaaa acaagaaatt 360attgatgctc ataagccaca
aagccttgct agtgcaactt tagccaaaag acgcggactt 420gttattgtta
aaaagaaaaa agacgaagaa gaaattcaag ttaaaaaaga agaagtaaaa
480aattcaaatg atatatctat caataatgaa gagcgcttaa gtttaaaaac
tatgttttca 540aatgctgatg agagtttaaa gaaaaagaaa aaagaaaaaa
aatcttttgt tgcgagtaaa 600aaagaaagta ccgaaaaaat gaatttttta
gatgaacatg attttggtga tatttcttta 660gatgatgaag atgaggtagt
attacctgat tttagtgtaa aagaacaaga aaaaccacaa 720aatatcaata
aaaaacaacc taattttata agacaagctg ttggaaattc tgcgggtttt
780gggtttgaag gtggaattca aagaagaagt cgtaaaaaac catctaaaaa
gattgaaaaa 840aaggaagtag aagaagtagg tagcgttgct atttctaaag
aaattcgtgt gtatgaattc 900gctgataaga taggaaaaag cactagtgaa
gtgatttcaa aacttttcat gcttggaatg 960atgacaacaa aaaatgattt
cttagatgaa gatgcgattg aaattttggc tgctgaattt 1020ggtatagaga
tcaatatcat taacgaggct gatgagtttg attatgtaaa agactatgaa
1080gaagagactg atgaaaaaga tttagtgact agagcacctg tgattaccat
catggggcat 1140gttgatcatg gtaaaacttc tttgttagat tatattagaa
aatcacgcgt tgcaagtggt 1200gaagcagggg gtattactca gcacgtgggt
gcttatatgg tagaaaaaaa cgggcgtaaa 1260attactttta tcgatactcc
aggtcacgaa gcttttactg ctatgcgtgc aaggggtgca 1320agtatcactg
atattgtaat catcgttgta gccgcagatg atggggtaaa accacaaacc
1380aaagaagcga taaatcatgc taaagcagca ggtgtgccta ttattattgc
tattaataaa 1440atggataaag aagcagcaaa tcccgatatg gtaaaaactc
aactcgcaga aatggaaatc 1500atgccagtag aatggggcgg atcttatgaa
tttgtaggag tttcggctaa aacaggaatg 1560gggattgaag atttgcttga
aatcgtgctt ttacaagctg atattttaga acttaaagcc 1620aatccaaaaa
gttttgctaa agcaagcatt atagaaagtt ctgtgcaaaa agggcgtggt
1680gcggtggcta ctgtcatcgt gcaaaatgga acacttactg taggaagtac
cgtggttgca 1740ggcgaggctt atggaaaagt gcgtgcgatg agcgatgatc
aaggtaaagc cttaaaagaa 1800attaaaccag gcgaatgtgg ggttatcgta
gggcttagtg aagtagcaga tgcaggtgaa 1860attttaatcg cagtaaaaac
cgataaagaa gcaagagaat atgctaataa acgccacgaa 1920tacaatcgcc
aaaaagaact tagcaaatcc actaaagtta gcattgatga gcttggagct
1980aagatcaaag aaggtaatct aaaagccttg cctgttattt taaaagctga
tgtgcaagga 2040tctttagaag ccttaaaggc aagtttagaa aaacttagaa
atgatgagat taaagtgaat 2100atcattcata gtggagtagg aggaatcacg
caaagtgata tagagcttgc aagtgcgagt 2160gaaaactcta tagtactagg
ttttaacata cgcccaacag gggaagttaa agagcgtgct 2220aaggataaag
gcgtagaaat taaaacttat aatgtaattt ataatctttt agacgatgta
2280aaagccttac ttggtggtat gatgagcccg attatttctg aagagcaatt
aggacaagca 2340gagattagac aagtgatcaa tgtgccaaaa atcggacaaa
tcgcaggttg tatggtaact 2400gaaggggtga ttaatcgtgg agccaaaatt
cgccttatcc gtgatggagt tgtggtttat 2460gaaggaaatg taagttcgct
taaacgcttt aaagatgatg ctaaagaagt ggcaaaaggc 2520tatgagtgtg
gcgtaggtat agaagggtgc gatgatatga gagtgggtga ttatatagaa
2580agctataaag aagtagagga acaagcaagt ctatga
2616522841DNACampylobacter jejuni 52atgctagcac ctggcatggg
agaatgggtt tacaaggcta atttattttt atttggagaa 60tttgcgtatt attatccttt
tttcttattt attttaaatt atgtttatta taaaagaaat 120tacaaattag
ctaattttac aagaagagag ctttttggta taggatttgc ctttttctcg
180agtttgcttt tatttgcagt tttttatcct aattcaggct atatactaga
gcttgcttat 240gcgatttttt ctactatttt ggggcatact gggagcggaa
ttttcgctct tttacttcta 300ctattttctt tggttttatt gtttccaaaa
tttgcaaaag aaattttaaa aatagaatta 360gattttactt atttattaaa
agtagagcaa gcttttaaat ctttacttat gcgtgtattt 420ggtggagaaa
acgagaaaga agatgtaggt aaatcagaac ctatagttcc aaaattaaat
480attttgcaag atagtattta tggaaattta caaataaaca aaaaagggga
aaccaataac 540ttagaacaga taattaaaga tagcaatatc aatgcaagta
aaaattcaat taccacggcc 600aaggaaaatt ttgaaaaact aaaaaatcaa
attttagatg aaaccataga gattgataaa 660caaagtttaa aagaatcaag
aagttttgtt catgagcatt ctcaacaagt gcgaaatttc 720gcacaaaagg
ctagtaaaat gagcattagt cttgatgaag attttaattt tatttcagaa
780gaagaggtag atatgattcc tgaacgtttt ttaaaaccta aaaaacttga
agatataaag 840caaatagata caaataaaaa tttagatgag ccaagttata
aacgtaaaaa tattgaaatt 900ccagtttcta atcaagaagt taaaccaaaa
atttttacaa aagagcttga acttagagag 960aatttgataa aaaaagaaaa
actagaacaa gaatacaaag cctatcaaaa tgaaatttta 1020gaaaataaag
taaaacaaga aattaaaaaa ttagaagaat atgatgcgat aaattcaagc
1080gatattatag aagggaataa atatagtttt aatagcccaa aaacaattaa
aacagaaaca 1140gaagaatcag acaaaataaa tgaaaataaa aatctagata
aagcagacaa tatctttgaa 1200tttgctccca ttgtagaaga gttaaatcat
ccttatatag aacctactcc tataaaaaat 1260ataaatgaaa tagttataga
agaaaaaaat acactcgatt ttatccaaaa tacagaaact 1320aaaatcgata
atgaaaaaac aaatgatcaa gaaattaaac ttcaaaaagc agttttagcc
1380aaagaaattg ctattaacca agctttattg cgtgaaatag agcagggcga
aatagaaaaa 1440ccaaaagatt tcaccttgcc gccattagat tttttagcta
atccaaaaga acacaaacaa 1500gaaatcaatg aaagtgaaat agataaaaaa
atttataatc ttcttgaaaa attgcgtcgt 1560tttaaaatag gcggcgatgt
tataagcact tatgttggtc ctgtggtaac tacttttgaa 1620tttcgtccta
gtgcagatgt gaaagtaagt cgtattttaa atttacaaga tgatttaact
1680atggctttaa tggcaaaatc aatccgtatt caagctccaa taccaggaaa
agatgttgta 1740ggtatagaag ttccaaatga tgaaattcaa accatttatt
taagagaaat tttacaaagt 1800gaagttttta aaaacgctaa aagtccttta
accatagctt taggtaaaga tatagtaggc 1860aatgcttttg taaccgatct
taaaaaactt ccgcatttac tcatcgcagg aacgacaggt 1920agtggtaaaa
gtgtggggat aaattctatg cttttaagtc ttttatatcg caactctcca
1980aaaaccttgc gtttgatgat gatagatcct aagatgcttg aatttagcat
ttataatgac 2040attcctcatc ttttaactcc tgttatcaca gatccaaaaa
aagcagtcaa tgcgctttca 2100aatatggtag ctgaaatgga aagacgctat
cgcttaatgg ctgatgcaaa aaccaaaaat 2160atagaaaatt acaatgaaaa
aatgaaagaa ttaggcggag aaaaacttcc ttttattgta 2220gtaattatcg
atgaacttgc tgatttgatg atgactgcgg gtaaagatgt agaattttat
2280ataggaagac ttgcgcaaat ggcaagagca agtggaatcc acttgattgt
agctacacaa 2340cgaccttctg ttgatgttgt aacaggacta attaaagcga
atttaccaag tagaatttct 2400tataaagtag ggcaaaaaat tgactctaaa
gttattttag atgctatggg tgctgaaagt 2460ttacttggaa gaggggattg
tttatttact cctcctggaa caagctctat agtgcgtttg 2520catgcgcctt
ttgcaagcga atttgaaata gaaaaaatcg tggattttct aaaggatcaa
2580caaagcgtag aatatgatga aagcttttta aaggatcagc aaagcgtagg
tgttacaaca 2640aatgaaagct ttgatgggga agcagatgag ctttatgaag
aagctaaaag agtaatctta 2700gaagatggaa aaacaagcat ttcttattta
caacgccgtt taaaaatcgg ctataaccgc 2760tctgcaaata ttattgaaca
acttacgcaa aacgggattt taagcgaacc tgatgccaaa 2820ggacaaaggg
aaatcttgta a 2841533126DNACampylobacter jejuni 53atgaaaaaat
ttttttgttt aactttagtt tgtaaacttt ttgctttaag cgaatttgaa 60cttcatcata
ttgataaagt acataagcta gggtatagcg gagatactat tataataggt
120gtagccgatg atgcttttaa tcaagatcat attagtttaa aggataagat
tttaaagtct 180acttatccta ctgatacagc tgggaaacag cttatacctg
atttgaaaaa atcaacacac 240ggaagtcatg tagcaggtat agctgttgga
gcaaagatag gcgatagcaa accttatgga 300gtggcttatg gggcaaaatt
ttatggagct ggggtgtttc caaatggctc ttacactcaa 360attcctgata
tttataattt tttcaaagat gtgagtatta ttaataatag ctggggtatt
420aatttttatc cttattttaa tcttaaggct tcaaattctg gattagtaga
ttgtactcaa 480actaatcaag ggacaagcta taatatctgt aatactcctt
tggaatatgt tatgaaagca 540gataaggttg ctaatgatat gatgaggctt
agcaaggaca agggagtatt aaatgtattt 600gctgctggta atgaaggaat
tcttagccct gctttgcatg cgattttacc aagttatgat 660gaatctttaa
gagcttggtt ggctgttgga gctttggatg caaatgagat tactttagaa
720tcagatggga ctttaataat taaaagtcaa ggtttggctg attttagtaa
tggttttaag 780ggagctacga atttttcttt agttgctgct
ggagtaaata ttaataatgt tgattcaagc 840actaatgata agtttacaaa
aaaaagtgga acttctatgg cagcacctat ggtaagtgga 900acagcggcac
tggtcaagca aaatttcccc tttttggatg gtaagcaaat tgctgatata
960ctattaagta cagcaaataa aaattataag gctccaaaat ttactgttaa
acaagtaacc 1020gatggaacaa atcaacctaa atttcttatt gtgtatattt
cgcaagatcc acctgggata 1080gaagatgaaa taaaacggga tttaaaacag
ctttacaatg gaatacaagt tcaagttaat 1140ggacaatgga ttgattatag
tgattatatt tgggataata gagatagtgc gcagtcacaa 1200aaacttaata
cttccactat tagttctatt aatggagtag ttagagttga gaaagaagaa
1260ttatttggac aaggaatttt agatgcacaa aaggcgttaa aaggactgag
tattttagat 1320gcaaacagac tcagtgatca ggatgtatta aaatatgagc
aagaacctaa tacagcttat 1380tatactataa acactgcagg ttatgatgct
gaattttcta atgatattag tcaaagaaaa 1440tgggatgaaa gtactcattt
atcaagtgct attaataaac ctacacattt agctaatctt 1500aacatagggt
tatctaaaga aggtgaaggt attttgatta tcagtggtca aaatacttat
1560gagggcgcaa ctttaatcaa acaaggagaa ttaaagctta aaggaaaagt
taaaaataat 1620gcttatgtag aacaaaaagc aatattaagc ggtaatggta
ttgtagggca aaatttaaac 1680aataaaggca tagttagacc tggaaatgaa
gatttgaatg atttaaccgt gcaaggaact 1740tatactcaag aaggagttga
ttctaaattg caacttgatt ttggtaatta taaaaattct 1800aaactgattg
caaaaactta tgatattaag agcgggaatt tagaatatat tcctttacct
1860aaatactata tcttaaataa gccagtgaaa attaatttgg gagatttgga
aaaaagttta 1920tcatctttta atcatgtttt gatacaaaat acctatgctt
taaattttga ttttgtttta 1980agtgatgatt tagtgagtat taataaaacc
ttaataaaac ctaatttaaa gccaaatgct 2040tacgaaattc ctaatacaag
cttgggaaat gctttaagac aattgcgatc tagggcagac 2100ttgagtcaga
cttatcagga attttttgct tctttagata atggaataga tgtaaagact
2160aaattaaata gaatagaagg ttcagggtat ttaagcactt ttagtaatca
taatcaatct 2220aatttaatgc aaaataatat gttatttacc cttcatcctc
ttaatattaa taattttgca 2280caaaacaata atatcttact tgctagtact
tatttaccta gaatttttag caatgaagaa 2340tatttttggc atcttactcc
aagttataaa tactataaag ataaagattt ttcaggtcaa 2400aaaacaggtg
ctaatatctc tttaggagaa aatttctcat caggcttttt agcttatgct
2460ttatctcttt ctagcgctaa atttaatttt aataatggta gtgatttgaa
gagttataac 2520atggatttat tgcttaatta taaccatgat ttagatttta
taaaaatatt aagtggatta 2580ggtataggtg taggatttaa tactcttaat
cgttttgtag tagagcagcc aattgaaggc 2640aaatataaaa cattgcaaac
ttcagcccag cttggtgtaa ctaaagatat tattttaggt 2700caagatttta
tttttaatcc tttaatgtat tttacacata gtttttttta tcaagaagat
2760tttaaagaaa ataaaagtcc ttttgctaaa aattatgaaa gtttaaaaca
tcatagcata 2820aatgcaaatt taggttttaa tcttgctaaa aatatagagc
aagatgatta tcaagcttct 2880ttttctactt ttgtaatttt tgaaaaaaga
atttatggaa gaactttaga aaataaggct 2940agttttgttg attttcctat
tgcttttatt caaaaatata aattaaaaga taatatttta 3000agtcaaggtt
ttaattcaga atttttatat aaaaacaatg tattttggca gtttatgtta
3060atgaatagat tttctcataa tgcctatgaa ttgcatttaa tgagttcagt
aggaaaacgt 3120ttttga 3126543270DNACampylobacter jejuni
54atgccaaaac gaacagatat taaaagcatt ttacttatag gaagtggtcc tattgtgata
60ggacaagctt gtgaatttga ttattctgga actcaagccg caaagacttt aaaagaatta
120ggatatcgtg tagtattaat caactcaaat cctgcaacca tcatgacaga
tcccgaattt 180gcagatgcga cttatataga acccataaca aaagaaagta
ttttaagtat tattaaaaaa 240gaaaaaattg atgcaatttt gccaactatg
ggtggacaag tagcgttaaa tgttgctatg 300gaagtttatg aaagcggact
tttaggagat gtgaaatttt taggcgcaaa tcctgaggcg 360attaaaaaag
gcgaagatcg tcaggttttt aaagaatgta tgaaaaaaat tggcatggat
420ttgccaaaat cgatgtatgc gtataattat gacgaagctt taaaagccgt
agatgaaatc 480gactttcctt tgatgatccg tgcttcttat actttagggg
gtgctggaag tggtgtggtt 540tacaatatgg acgaatttaa agaacttacc
aatactgctt tagctttatc acctattcat 600gaaattttga ttgaagaaag
tttgttaggt tggaaagaat atgaaatgga agttatacgc 660gatagagcgg
ataattgtat catagtttgt agcatagaaa atatcgatcc tatgggagtt
720catacaggag atagtattac aatagctcca gcattaactt tgacagataa
agaatatcaa 780gttatgcgta atgcttcttt tgctattttg cgtgaaattg
gtgtagatac aggcggaagt 840aatgtgcaat ttgctatcaa cccaaaaaat
ggaagaatga tagttataga aatgaatcca 900agagtttcaa gatcaagtgc
tttagcttct aaggcaacgg gttatcctat agcaaaggtt 960gcgacacttt
tggcagtagg ttttagctta gatgagatta aaaatgatat tacaggaact
1020cctgcatctt tcgagcctgt gattgattat attgtaacaa aaattcctcg
ctttaccttt 1080gaaaaatttc caggagcaaa tacaacttta ggtacagcta
tgaaaagtgt gggtgaggta 1140atggctatag gacgcacttt taaagaaagt
atacaaaaag cactttgttc gcttgagcgt 1200tctttaagtg gttttgatag
ggtaaaattt gaagatagaa atgatcttgt ttttaaaatt 1260cgcaatgcca
atgaaaagcg tttactttat gttgctcaag cttttaggga aggttttagc
1320gtagaagaac tttatgagct ttgtaaaata gatccttggt ttttaacaca
gattaaagaa 1380attgtagatt ttgaagaaca aattgatatg gatattttaa
acaataaggc tcttttgaga 1440aaagcaaaaa ctatgggctt ttcagataaa
atgatagcct tgcttgtaaa tttgaaagat 1500aatttagaat taagccaaaa
tgatatttat tatgtaagaa tgaagcaaaa aatcatcgca 1560gaatttagtg
aagtggatac ttgtgcgggt gaatttgaag ccttaactcc ttatctttat
1620tcaagtatca atgtaagcga actcactcaa agtaaaaacg atgctaagga
taaaaaagaa 1680aaaaaagtga tgattatagg tggggggcca aaccgtatag
gacaaggtat agaatttgac 1740tatgcttgcg tacatgcttc ttttgcgctt
aaagatatgg gtattaaaac tattatgtat 1800aattgtaatc ctgaaaccgt
ttcgactgac tatgatacaa gtgatatttt gtatttcgag 1860cctattgatt
tcgaacattt aagagcggtg attgagcgtg aaaaacctga tggagtgatt
1920gtgcattttg gtggacaaac tcctttgaaa tttgctaagc gtttaagtgc
ttttggagct 1980aagattatag gtactagcgc aagagtaatt gatatggcag
aagatagaaa gaaatttgcc 2040gaatttatta caaagctagg tatcaatcag
ccaaaaaatt ctactgcaac aagcgtagaa 2100gaagcggttc ttaaggctag
tgatataggg tatcctgtgc ttgtaagacc aagttatgtt 2160ttaggtgggc
gtgcgatgcg cgtggtaaat gatgaggctg aacttagact ctatatgcaa
2220gaagctgtgg atgtaagcga taaaagccct gttttgatcg atcagttttt
agacaatgct 2280acagaaattg atgttgatgc gatttgtgat ggcaaagatg
tttatgttgc aggaattatg 2340gagcacatag aagaagcagg aattcattcg
ggtgacagtg cttgttcttt gccgccttgc 2400aatatcgatg aaaaaatgca
agaatttatt gcacaaaaaa ccgcagatat tgctttaaat 2460ttgggagttg
taggactttt aaatatacaa tttgctttac ataataatga gctttatatg
2520atagaggtaa atcctagagc tagtcgtacc ataccttttg ttagtaaagc
tacgggtatt 2580cctttagcaa aagtggcaac gcgtgtgatg tggcaaggaa
atttaaaaga agctttaaaa 2640ttttatgata cttttaaagt ggttaatttt
gatactaaaa ttttacgccc taaaactcca 2700aaatatatga gcgtgaaaga
agcagtattt ccatttgcaa aacttagtgg aagtgattta 2760gaattaggtc
ctgaaatgcg ttcaacgggt gaagttatgg gtataagcaa ggattttgca
2820aattcttatg cgaaaagtca aattgcatcg tttaatcatc ttccagagca
aggcgtggta 2880tttatctcct taaaagataa ggataaaaaa tataccaaaa
aaatcgctgc agaatatgta 2940aagcttggct ttaagcttat ggcaacaggg
ggaacttgca aggaaatttt agaaagtggt 3000tttgagtgcg aacttgtaca
taaaatttca gaaggacgcc ccaatgttga agataaattg 3060aaaaatggag
aaattcactt agttatcaat acaagcgata gtcacagttt taaaggcgat
3120acgaaaaaaa ttcgtgaaaa tattattcgt tttaaaatac cttattttac
aaatttacga 3180tcagctttag caggtgcaaa atcgattaaa gctatacaga
gtaaatcttg cctagatgta 3240aagagtttgc aagagtggct taaatcttga
3270553365DNACampylobacter jejuni 55atgaaaaata tcacattaac
taaaatacct ataggagagg gcaaagaacc ctgtttaaat 60tctaaaaaga tagttttatc
tttagctact atttcttttt tggcaagttg tgctaatgct 120aaattaaatt
ctgaaataaa aacttatgat gaagttaata aaaatgttaa aactagatca
180gcaagtgttt atagccctca agctaagatc aatacgacta taaattcttt
acacaatcaa 240caagtaacta ttactggaaa tggaacatca aattctttaa
caatcggatc aagtggaact 300ctaggtagca taggcaatac aggtaaaatc
atctatgccc atgctaatgg tagcaacact 360cttactcttg caaatcttac
taataataga actattaatg gtaagattgg tatagaaaac 420aatggtaatt
ttacaggaac tattgctgtc aacacctttg aaaatacagg tcaaattaat
480ggacaaattt acatgggaat ttggggtaac aactcaggaa ctcttaatat
agataaattt 540gacaatagtg gaaccataat tgacaacaat aaaggagttt
ttttgaagga aagaatacca 600acatacaaac ctttaataac agtggtttta
ttagtgcaaa taagggtgta gatataggca 660acataggaac cataaaaaat
tttaataaca acggaactat acaaggctca gaagtgggag 720tggctataaa
tacaaaaata gatactttta ctaataatgg ttttattaat tcccctggta
780gtggacaatg gaataatggt atatggataa gtagcaatgc taccatagaa
aagcttgtta 840acaatggcac aataaaagga ggacattctg ctataatggt
aacatctcag catataaaaa 900ctgttgaaaa tacaggaatt atacatgctg
aaggagaatg gggttctagt atattattag 960aatatggtgg ttttatagag
catataatca atactggaac cataagtaat aataatgttg 1020gtataggttc
agcttatgga gtatttggaa cacttaccat aaaagatgga ggtatggttt
1080atggaaaata ctcggcaata ggagtgggtc gatcgcaaac tctaggagat
ttatatatag 1140atggacgttc aaataatggc acagtaagtg gaatttatag
tgaagaacat ggaattttat 1200tagagaataa ctcacgaact caaaaaatag
aacttaaaaa tggcggcatt ataaaaggta 1260atatcgatgg tataagattg
ataaactcgg cctctttaag tggagaaatg attttatctg 1320gcgaaggttc
tagggtagaa ggtggaagag gtgttggtat attgaatcga agtggaaaaa
1380tagaaggctc tataaaagta gaagatggag caactgttac ggctacttcg
aatcgagcca 1440tagctaactc tggctcagga agtataacag gtggtattac
tgttagtggg aaaaacacta 1500aacttgaagg aaatataatc aatacaggta
atgcttctat aggtagtgat atcaagatag 1560aaggtggagc taaggtagaa
ggtggtcttg ttaatcaagg taatggaagt atctcaggaa 1620gtgttcaagt
aagtggtggt agtagtattg attccattac caatgagggt aatggagcta
1680tttctggttc aattactgta tataaagata gtaaacttga ttctatcact
aacacttcta 1740catcatctac aggtataagt ggttctatta ctaacaatag
tgataataaa cttgaaattt 1800ctaattcagg taatatcggt ggtaagattg
aaagtacagg tagtgctgat atggtgatta 1860gcaatagtaa tggtggaact
attagtggcg gtattagttc ttcaggaagt ggaagcacta 1920gtatttccaa
ttcacaaggc tcaaccataa ataacggcat cactgtttca ggatcagcac
1980aagttgaaat ctccaatcaa ggctcagtag gtaaagatga aaatggtaat
acagtaacta 2040acaatggtag tggtagtgtt ggaatcaaag attggcttgt
ttctacagat aaaaacacag 2100gtaaattaaa cactgtggtt ataggtggaa
gtagagcttt taatgtaaaa gtagaaaaca 2160tcaccgtaga tcaaagcaat
gttgatcttg aagagctaaa cgatataaac aacatcatct 2220caggtgttaa
tcaaaacaat attggcaata taggaaccaa tggaagtgga gaaatatctt
2280taagctttga tccaataaca ggaaagctta ctacagattt taatcttaat
gcttccatat 2340caggtgcaac ctttagatct ttaatctcta ctacttcaag
aagatcaacc tttatagata 2400atgttatggg taattctatg caaagctttg
ctttagcttc ttcaagtaaa tctcaaagca 2460tcgctatgtc tgaaaagggt
aatttatatg cagatgcaag tgattatata aagagtgatt 2520taaataatgg
aagctatggt tctaataaag aacattcttt attcatcctt ccttatactt
2580cttcacagaa tgttgaactt tctttaaatg aagaaagtaa aggacacact
aaaggaacca 2640ttataggtta ttctacttta aaagacagtg gaatatatgg
tgtgtatgca ggttatgaag 2700atacaaaaat gggttcaact tattttgata
ttaataatag aacctattat gcaggtttaa 2760aatactttaa taccttattt
actacagaaa aaggtcaaga agtttatatc aaagctcaag 2820gtaaagctgc
tttgattaaa aatgatttaa ctgaaaaaat aggaaataat gaagctaaag
2880ctgaacctaa ttcttatgct tatggagtca atacagcctt aggtatgaat
tttatttcta 2940ataaagatat attctctcct gaaataggtc ttgcttatga
aggaggttat actgaagctt 3000tttctatgaa agacaccata ggacaagcaa
cagttaaagg tggagaaaga acctatgcaa 3060actatttaaa tcttttctct
actaaaacaa gtcttacttg gtttagagat tggttgccta 3120atttaaaaac
ttcagtagaa cttggagcta agtttaatat caatcctaaa gtagaagctg
3180aggctagatt tggtaatata aaagtaagtg atgagtttga tttaccaaga
gtacaaaaat 3240ttgtaagcac ttcttttatc gttcctgtta atgaagcttt
ctattttagt ttaaactata 3300atggaatgtt tgataaagat ggcaataccc
atacaggatt tgctcagttt aattatcttt 3360ggtaa
3365563436DNACampylobacter jejuni 56atgaataaaa ctgcattaac
taaaacatac acaaaagata tacaaaattc ctgtttaaat 60tctaaaaaga tagttttatc
tttagctact atttcttttt tggcaagttg tactcatgct 120acacttactc
ctgaaataaa aacttacgaa gagacaaata gacatgctaa agctaggtca
180ggtcttcaat ctagaaattc gaataatgag actataaata atttacaaac
tttaactaaa 240actataagtg acaccggcaa tactctagtt atagaaagta
gtggaactat tactatttct 300aatgatgggc aacaagcagt aaactttcaa
ccaaattctt caacttctac ctttttaaat 360aaaggaactc ttataggtgg
aaataatact gctagtgttc aactaggagc agcaaatgga 420aataacggtg
ttagcataga aacctttaat aatcaaggta tcataggtaa tggttcttct
480aaatttggag taacagtttt tggggggggg gagtaaagat aaccctaaat
ctataatcaa 540taattttagt aatagtggaa ctattcactc taatactggt
gaaagtattt attttggtaa 600cgccaaaata tcaagttttg ttaatagtgg
gactattaag agtaaacaag gtgcaggagt 660aaatatatct caaggaacaa
gtatagagaa ttttaacaat accggaaccg gaattattga 720aggcaagaga
atgggtgtaa atgttcgttc aacaataaat accttcgtca atgacggttt
780aattgctgca acgaacgatg gaatacagat aaatgctaat gtaaaaacat
taataaataa 840gggaaccata aaaggagatg ctatatctat aagatcatta
ggtggaacta tagaaacatt 900gaccaatgaa ggcattatgt atggtaaaag
tgctggtatt tacatgaaca gaagtcttgt 960taaaactctt acaaatagcg
gtaccattaa tcaaaacaat tcggcaactt ggtctgctgg 1020tataaaactc
gaaaatggta gtatcataga aaatatcatc aacacaggat ctatccgttc
1080taatgctttt ggaatatctg taactggtgg taaatttgga acacttacca
taaaagatgg 1140aggtatggtt tatggaaaat actcggcaat aggagtgggt
cgatcgcaaa ctctaggaga 1200tttatatata gatggacgtt caaataatgg
cacagtaagt ggaatttata gtgaagaaca 1260tggaatttta ttagagaata
actcacgaac tcaaaaaata gaacttaaaa atggcggcat 1320tataaaaggt
aatatcgatg gtataagatt gataaactcg gcctctttaa gtggagaaat
1380gattttatct ggcgaaggtt ctagggtaga aggtggaaga ggtgttggta
tattgaatcg 1440aagtggaaaa atagaaggct ctataaaagt agaagatgga
gcaactgtta cggctacttc 1500gaatcgagcc atagctaact ctggctcagg
aagtataaca ggtggtatta ctgttagtgg 1560gaaaaacact aaacttgaag
gaaatataat caatacaggt aatgcttcta taggtagtga 1620tatcaagata
gaaggtggag ctaaggtaga aggtggtctt gttaatcaag gtaatggaag
1680tatctcagga agtgttcaag taagtggtgg tagtagtatt gattccatta
ccaatgaggg 1740taatggagct atttctggtt caattactgt atataaagat
agtaaacttg attctatcac 1800taacacttct acatcatcta caggtataag
tggttctatt actaacaata gtgataataa 1860acttgaaatt tctaattcag
gtaatatcgg tggtaagatt gaaagtacag gtagtgctga 1920tatggtgatt
agcaatagta atggtggaac tattagtggc ggtattagtt cttcaggaag
1980tggaagcact agtatttcca attcacaagg ctcaaccata aataacggca
tcactgtttc 2040aggatcagca caagttgaaa tctccaatca aggctcagta
ggtaaagatg aaaatggtaa 2100tacagtaact aacaatggta gtggtagtgt
tggaatcaaa gattggcttg tttctacaga 2160taaaaacaca ggtaaattaa
acactgtggt tataggtgga agtagagctt ttaatgtaaa 2220agtagaaaac
atcaccgtag atcaaagcaa tgttgatctt gaagagctaa acgatataaa
2280caacatcatc tcaggtgtta atcaaaacaa tattggcaat ataggaacca
atggaagtgg 2340agaaatatct ttaagctttg atccaataac aggaaagctt
actacagatt ttaatcttaa 2400tgcttccata tcaggtgcaa cctttagatc
tttaatctct actacttcaa gaagatcaac 2460ctttatagat aatgttatgg
gtaattctat gcaaagcttt gctttagctt cttcaagtaa 2520atctcaaagc
atcgctatgt ctgaaaaggg taatttatat gcagatgcaa gtgattatat
2580aaagagtgat ttaaataatg gaagctatgg ttctaataaa gaacattctt
tattcatcct 2640tccttatact tcttcacaga atgttgaact ttctttaaat
gaagaaagta aaggacacac 2700taaaggaacc attataggtt attctacttt
aaaagacagt ggaatatatg gtgtgtatgc 2760aggttatgaa gatacaaaaa
tgggttcaac ttattttgat attaataata gaacctatta 2820tgcaggttta
aaatacttta ataccttatt tactacagaa aaaggtcaag aagtttatat
2880caaagctcaa ggtaaagctg ctttgattaa aaatgattta actgaaaaaa
taggaaataa 2940tgaagctaaa gctgaaccta attcttatgc ttatggagtc
aatacagcct taggtatgaa 3000ttttatttct aataaagata tattctctcc
tgaaataggt cttgcttatg aaggaggtta 3060tactgaagct ttttctatga
aagacaccat aggacaagca acagttaaag gtggagaaag 3120aacctatgca
aactatttaa atcttttctc tactaaaaca agtcttactt ggtttagaga
3180ttggttgcct aatttaaaaa cttcagtaga acttggagct aagtttaata
tcaatcctaa 3240agtagaagct gaggctagat ttggtaatat aaaagtaagt
gatgagtttg atttaccaag 3300agtacaaaaa tttgtaagca cttcttttat
cgttcctgtt aatgaagctt tctattttag 3360tttaaactat aatggaatgt
ttgataaaga tggcaatacc catacaggat ttgctcagtt 3420taattatctt tggtaa
3436573561DNACampylobacter jejuni 57atgggtaaaa ttatgaaaac
tatggacgga aatgaggctg ctgcatacgc tgcgtatgct 60tttactgaag ttgctggaat
ttatcctatt acacctagtt ctcctatggc tgattatacc 120gatatgtggg
cagctgcagg aaaaaaaaat ctttttggag ttcctgtaaa aatcgtagaa
180atgcaaagtg aagcaggagc tgcaggtagt gtgcatggat ctttacaagc
tggggctttg 240actacaactt atacggcttc tcaaggatta ctccttaaaa
ttccaaatat gtataaaata 300gcaggtcaat tactcccctg tgtaatccat
gtagcagcgc gttctttggc tgctcaagct 360ttgtctatct ttggtgatca
tcaagatatt tatgcagcaa gacaaattgg ttttgctatg 420ctttgttcgc
attctgtgca agaaaccatg gacttagctg gtgtagcaca tttagctgca
480attaagggaa gagtgccatt tttacatttt tttgatggtt ttagaacaag
ccatgaaatt 540caaaaagttg aagtaatgga ttatgcgcat tttgatagat
tattagatag agaagctttg 600cttgaattta gaaataatgc tttaaatcca
gaaaatccaa aaacacgtgg aacagcacaa 660aatgatgata tttattttca
aactagagaa gtaagcaatc gtttttacga tgctttacct 720gatgttgtta
atgaatatat gcaagaaatt tcaaaaatta caggtagaga atacaaacca
780tttacttatt atggtcacaa agagccagag tgtgtgattg ttgctatggg
ttctgtaact 840caagcacttg aagaagtggt ggactatctt aatgccaagg
gtgaaaaggt agggatttta 900aaagtttatc tttatcgccc atttagttta
aaatatttct ttgatgttat gccaaaatca 960gtgaaaaaaa tcgcggtttt
agatagaacc aaagaaccag gaagtctcgg agaaccactt 1020tatcttgatg
taaaatcagc tttctatgga agagaaaatg ctcctgttat agtgggtgga
1080agatacggac tttcttcaaa ggatgttgat cctgctcaaa tgatagcagt
atttgaaaat 1140cttaaacttg ataatccaaa agatggcttt actgtaggta
taattgatga tgtaactcat 1200acttcactga gtactggaga gaaaatttca
cttggagatg aaagtacgat cgaatgttta 1260ttttacggac ttggtgctga
tggaactgtg ggtgcaaata aaaactcgat taaaatcatc 1320ggagataaaa
cggattttta cgctcaagct tattttgctt atgattctaa aaaatcaggg
1380ggttatacaa gaagccattt aagattttct aaaaaaccta tccgttcaac
ttatcttgtt 1440tcaactccgc attttatcgc ttgttcggta gcggcttatt
tggaaattta tgatgtttta 1500gcaggaattc gcaaaggagg aactttcctt
ttaaatagta tttggaatgc tgaagaaacc 1560ataagacaac ttccagatgc
agtaaagaaa actttagctg aaaaagaagt aaatttttat 1620atcatcaatg
caaccaaact agctcgtgat ataggtttgg gaaatcgtac aaataccatt
1680atgcaatcag cctttttcaa acttgcaaaa atcattcctt atgaagacgc
acaaaaatac 1740atgaaagagc ttgcgtataa atcttatagt aaaaaaggcg
atgctattgt agaaatgaat 1800tacaaggcta ttgatgtggg tgctgatgga
cttgtaaaag ttgaagtgga tccaaattgg 1860aaaaatttag agcttaaaga
aaaagaacaa accaatgcct ataaaggtac tgaatttgtt 1920gaaaaaatcg
taaaacctat gaatgcggct aagggtgatg atttacctgt ttcagccttt
1980ttaggttatg aagatggaag ttttgaacac ggcacaaccg aatacgaaaa
acgcggtgtt 2040ggggttatgg taccaagatg gatagaggca aattgtattc
aatgcaatca atgtgcttca 2100gtttgtccgc atgctgttat caggccattt
ttgattaatg atgaagaaat ggcaaatgca 2160cctcgcggtg taaaagatca
tgctttagaa gctaaaggaa ccaaaggaga aaaattaagc 2220tttaaaattc
aagtttctcc acttgattgt acaggctgtg agctttgtgt tcatgagtgt
2280cctactaaag aaaaatcttt ggttatggta ccacttcaag aagagatgga
ttttggtgag 2340caagaaaatg cggattattt atttaaagaa
attacttata aagatgatat tttaaataaa 2400gaaaccacaa aaggagcgca
atttgcccaa cctttatttg aattccatgg ggcatgtcct 2460ggatgtgggg
aaactcctta tattacttta attacaagat tgttcggtga aagaatgatt
2520gtggctaatg ctacaggttg tagttctatt tatgggggtt cagctccatc
aactccttat 2580agaaaaagtg tgaaaaatgg acacggtcct gcttggggaa
attcactttt tgaagacaat 2640gctgagtttg gtttgggtat gaaaattgca
actgaaaata caagacaccg cattgaacat 2700atcatgaatg aaagtatgca
agaagttcca aatgctttat cagctctttt taaagattgg 2760attgcaaata
aagacaatgg tgctatgtct gtggaaatta aagataaaat gatccctatt
2820ttagagcaaa ataaaaatat taaagctgta caagatatat tagagcttaa
acagtattta 2880agtaaaaaat ctcactggat ttttggtggt gatggttggg
cttatgatat aggctatggc 2940ggacttgatc atgttttagc aagtggagaa
aatgtaaata ttttagtgct tgatacagaa 3000gtttattcta atacaggcgg
tcaaagttca aaatcttcta gaacaggagc tgtagcacag 3060tttgcagcag
caggcaaacc tatacagaaa aaagatctag gtcaaattgc tatgacttat
3120ggttatattt ttgtagcaca agtaaattca acggcaaatt atactcatct
tatcaaagct 3180atcactgcag ctgaggccta tgatggacca tctttggtga
tttgttattc tccttgtata 3240gctcatggta ttaaaggtgg gctgggttac
tcaggagagc aaggtgagct tgctacaaaa 3300tgtggttatt ggccacttta
tacctttgat cctcgtttag aagagcaagg aaaaaatcct 3360ttaactctaa
caggaaaaga acctgattgg gatttatatg agcaattttt gatgaatgaa
3420gtgcgttata attcacttaa aaaagcaaat cctgaacacg ctgctgagct
ttttgaacgc 3480aataaaaaag acgctcaacg tcgctataga cagcttaagc
gtattgctat ggctgattat 3540agcaatgagg tcgaaagctg a
3561584137DNACampylobacter jejuni 58atgtgcgata tgttagataa
taaattagga aatcgtttaa gagtagactt ctcaaacatt 60tcaaaacaaa tagaaatccc
aaatcttcta caattacaaa aaaagagttt tgattatttt 120ttaaatcttg
ataatggtga aagtggtata gaaaaagttt ttaaatcaat ttttcctatt
180catgatccgc aaaatagatt gagtcttgaa tatgttagta gtgaaatagg
caagccaaaa 240tataccattc gcgaatgcat ggaaagaggt ttgacttatt
ctgtaaattt aaaaatgaaa 300atccgtctta ctctgcatga aaaagatgaa
aaaacagggg aaaaagttgg cgtaaaagat 360attaaagaac aagaaattta
tatccgcgaa attcctttaa tgacagatcg tgtatctttt 420atcattaacg
gggttgagag agtggttgta aatcaattac atagaagccc tggtgttatt
480tttaaagaag aagaaagttc aacggttgca aataagcttg tttatacagc
acaaattatt 540cctgatcgtg gttcgtggct ttattttgaa tacgatgcca
aagatgtttt gtatgtaaga 600atcaataagc gtagaaaagt tcctgtaact
atgcttttta gagctttagg gtataaaaaa 660caagatatta ttaagttgtt
ttatcctatt cagactatac atgtgaaaaa agataaattt 720ttaacagaat
ttaatccaaa tgattttatg gatagaatag aatatgatat taaagatgaa
780aaaggaaaaa tcgttcatca agctggaaaa agacttacaa agaaaaaagc
agaacagctt 840attaaagacg gtttaaaatg gatagaatat cctgtagaaa
ttttacttaa tcgttattta 900gcaaatccta ttattgataa agaaagtgga
gaggttttat ttgattcttt aactttgtta 960gatgagagta agcttgctaa
gattaaagag caaaaaagtt ttgatatagc aaatgacttg 1020gctaatggcg
ttgatgcagc gattattaac tcctttgccc aagatggaga aactctaaaa
1080cttcttaaac aaagtgaaaa tatcgatgat gaaaatgatt tagctgcgat
tagaatttac 1140aaagttatgc gcccaggtga acctgtggta aaagatgcag
cgaaggcttt tgtaaatgat 1200ttattcttca atcctgaaag atacgatctt
accaaggtag gtcgtatgaa aatgaaccat 1260aaattaggtc ttgaagtacc
tgagtatgtt actgttttga ctaatgaaga tattattaaa 1320actgcaaaat
atttaattaa agtaaaaaat ggtaaaggac atattgatga tagagatcat
1380ttaggaaatc gtcgtatccg ttctataggt gaacttttag ccaatgaact
tcatttaggt 1440cttgcaaaaa tgcaaaaggc gatcagagat aaatttactt
ctttaaatgc ggatcttgat 1500aaagtaatgc cttatgattt aatcaatcct
aaaatgatta ctacaactat cattgaattt 1560tttacaggag gtcagctttc
gcaatttatg gatcaaacca accctttaag cgaggttact 1620cataagcgtc
gtttgtcagc gcttggtgaa ggtggacttg taaaagaaag agcaggtttt
1680gaagtgcgtg atgttcacgc gacacattat ggtagaattt gtccagttga
aacacctgaa 1740ggtcaaaata ttggtttgat taacactctt tctacttatg
caaaagtaaa tgaacttggc 1800tttgttgaag cgccatatag aaaagttgta
aatggtaaag tgactaatga agttgtttat 1860cttacagcga cacaagaaga
aggcttgttt atcgcaccag cttcaactaa ggttgatgct 1920aaaggcaata
tagtagaaga atttgttgag gctaggcaag atggtgaaac tatacttgca
1980agacgcgaag aagtgcaatt aatcgatctt tgctcaggta tggttgttgg
agttgcagct 2040tctttgattc cgtttttaga gcacgatgat gcaaacagag
cgctaatggg ttcaaacatg 2100caacgtcaag ctgtgccact tcttactgct
tcagctccga tagtaggaac aggtatggaa 2160caaattattg cgcgtgatgc
ttgggaagct gttaaagcaa agcgtggcgg tgtggttgag 2220aaagtggata
ataaaagcat tttcatttta ggtgaagatg ataaaggtcc atttattgac
2280cattacacta tggagaaaaa tttaagaacc aatcaaaata caaattatat
tcaacaccct 2340attgttaaaa aaggtgatat agtaaaagca ggacaaatta
tcgcagatgg tccttctatg 2400gatcaaggcg aacttgctat aggtaaaaat
gctttaatcg cttttatgcc ttggaatggt 2460tataactatg aggatgctat
agttgtaagt gagagaatta ttcgtgaaga tacttttaca 2520agcgtgcaca
tttatgaaaa agaaattgaa gcaagagaac taaaagacgg tatagaagaa
2580attaccaaag atattccaaa tgtaaaagaa gaagatgttg cgcatcttga
tgagagcggt 2640attgcaaaaa ttggtaccca tatcaaacca ggtatgattt
tggtgggtaa ggtttctcca 2700aaaggtgagg ttaaaccaac tcctgaagaa
agacttttaa gagcgatttt tggagaaaaa 2760gcaggacatg ttgtgaataa
atccctttat gcaaccgctt ctttagaggg tgttgttgtg 2820gatgttaaaa
ttttcactaa aaaaggctat gaaaaagatg atcgcgcaat aaaatcttat
2880gataaagaaa aaatggcttt agaaaaagag catcatgata gacttttgat
gatggataga 2940gaagaaatgc ttcgtgtttg tgctcttctt tccaaagcct
ctttaaatag cgatcaaaaa 3000attggagata aaaattataa aaaaggacaa
actgcagata ttagcgaact tgaaaaaatc 3060aatcgtttta ctttaacaac
tttgattaaa gcttattcta aagagattca aaaagaatac 3120gatgatttaa
aaaatcattt ccaaaatgag aagaaaaaat taaaagcaga acacgatgaa
3180aagcttgaaa ttttagaaaa agatgatatt ttaccaagtg gggtaattaa
gcttgttaaa 3240gtttatattg ctacaaaaag aaagcttaaa gtcggtgata
aaatggcagg acgtcatgga 3300aataaaggta tagtttcaac tatagttcct
gaagtagata tgccttattt gccaaatggt 3360aagagcgtgg atattgcgct
taatccactt ggcgttccaa gtcgtatgaa tataggtcaa 3420attttagaaa
gtcatttagg tttagttgga cttagacttg gggatcaaat tcaagaaatt
3480tttgatagaa agcaaaaaga ttttcttaaa gaattaagag cgaaaatact
tgaaatttgt 3540tctattccaa gacttgcaaa cgaaaaagaa tttattaaaa
gcttaagcga tgaagagctt 3600ttaaactatg ctagagactg gagtaaagga
gttaaatttt ctactcctgt ttttgaaggt 3660gtaaatatag aagaatttag
caagcttttt aaaatggcta agatagatat ggatggcaaa 3720acagaacttt
atgatggacg cacgggggaa aaaattgcag agcgtgttca tgtaggatgt
3780atgtatatgc taaaactcca tcacttggtt gatgaaaaag ttcatgcaag
aagtacagga 3840ccttatagct tggttaccca acaacctgtg ggtggtaaag
cactctttgg gggacaaaga 3900tttggagaaa tggaagtttg ggcacttgaa
gcttatggag cggctcacac tttaagagaa 3960atgttaacca taaaatcaga
tgatgtagaa ggtagattta gtgcctataa agcattgaca 4020aaaggtgaaa
atgttccagc aacaggaatt cctgaaacat tttttgtatt aaccaatgag
4080cttaaatctc ttgctttaga tgttgagatt tttgataagg atgaagataa tgagtaa
4137594491DNACampylobacter jejuni 59atggatttag aaaatatcct
agaaaataat caaagcatag gtttatatca cccaaagaac 60gaacacgatg cctgtggtat
cgctgcagtt gctaatatac gcggtattgc ttcttataag 120gttatttgtg
atgctttaga aattttgatg aatcttgaac accgaggtgg tacaggagct
180gaagaaaatt caggtgatgg ggcggggatt ttaatccaaa ttccacatga
tttttttaaa 240actcaagaat tgggttttga acttcctaaa aagggtgatt
atgctgtagc acagatgttt 300ttatcaccca atactgatgc aaaagaagaa
gcaaaagaaa tatttttaca aggtttaaag 360gataaaaaac ttgaattttt
aggttttaga gaagtgcctt ttaatcctag cgatataggt 420gcaagtgctt
taaaagctat gccttatttt ttacaagcct ttgtaaaaaa accaagtaaa
480ataagtgcag gacttgagtt tgaaagagtg ctttatagta cgcgtcgact
tatagaaaaa 540agagctatta atgtgccaaa attttatttt tcaagttttt
cttcacgcac catagtttat 600aaaggaatgc ttctttcaac tcaattgagc
gatttttatc ttgattttaa agatgtcaat 660atgaaatcag ccatcgcttt
ggtgcattct cgcttttcga ctaatacctt cccaagttgg 720gaaagagccc
atccaaaccg ttatatggtg cataatggag agattaatac catacgcgga
780aatgttgata gcataagagc tagagaaggc ttgatgcaaa gtgagtattt
tgaaaattta 840gatgaaattt ttcctatcat agctaagctt agcagtgatt
ctgcaatgtt tgataatact 900ttagaatttt tagctctaaa tggtcgtact
ttagaagaag cttttatgat gatggtgcca 960gaaccttggc ataaaaatga
aaatatggaa agcaaaaagc gtgcttttta tgaatatcat 1020tctttattga
tggagccttg ggatggacct gctgctatag tttttactga tggagtgatt
1080atgggggcga gtttggatag aaatggtttt cgtccttcaa ggtattatct
tacaaaagat 1140gatatgctca tactttcaag tgaaacaggg gctttaaaac
ttgatgaaaa aaatatcaaa 1200gctaaaaaac gcttagaacc tggaaaactt
ttactcgttg atacagcaag aggtagggtt 1260atagctgata atgaaatcaa
agagcattat gctaatgcta agccttataa aaaatggctt 1320aaaaatttag
ttgaacttga aaaacaaaaa agcggagttt ataaacatca gtttttaaaa
1380gaagatgagg ttttaaaact tcaaaaagct tttggttgga gttatgatga
gcttaaaatg 1440agcgtggctg ctatggctca aaatggtaaa gaagctatag
cagctatggg tgtagatact 1500cctttggcta tactttctaa aacttatcaa
cctttgtata attattttaa acaacttttt 1560gcacaagtaa ctaatcctcc
tcttgatgcc ataagagaag agattgttac ttctacaagg 1620atttatctag
gaagcgaagg caatttatta aaacccgatg aaaacaatgc aaaacgcgta
1680aaaatagcct tgcctgtgat aagcaatgaa gaactttttg aagttaaggc
tttaaataaa 1740tttcaagtta aagaattttc tatactttat gattattcta
aaaaaacttt agaaaaggct 1800ttagatgaac tttgcgttaa gatagaagat
gaggttaaaa aaggtgtttc tattatcatt 1860ttaagcgata aaggagtgga
tgaaaaaaac gcttatatcc ctgctttgct tgctgtttct 1920ggagtgcata
atcatctagt aagaaaaaat ttaagaacac atacaagtct tatcatcgaa
1980agtggtgaac ctagagaaat tcatcatttt gcatgtcttt taggttatgg
tgcgactgtt 2040attaatcctt atttggttta tgagagtata caaaaactca
ttgccaataa agatttaaat 2100ttaagttatg aaaaggcggt agaaaatttc
atcaaggcaa gttcaagtgg tatagtcaaa 2160attgcttcta aaatgggggt
ttctaccttg caaagttata atggctctgc actttttgaa 2220tgtctgggct
taagctctaa ggtgattgat aaatacttca cttctacaac ttcacgcatt
2280gaaggtatgg atttagaaga ttttgaaaaa gaactcattg ctttacacaa
acatgctttt 2340aacgatacac ataaggcttt agattctaaa ggaattcatg
gttttagaag tgctaaagaa 2400gaacatttaa tcgatccgct tgtgattttt
aatcttcagc aagcctgtcg caacaaagac 2460tataaaagct ttaaaaaata
ctcggcttta gtagatgaaa aacaagttaa tttgcgttct 2520ttgatggaat
ttgattttag tgaagctatc agtatagata aggttgaaag tgtagaaagt
2580atagttaaac gctttagaac aggggcgatg agttatggtt ctatttctaa
agaagcacac 2640gagtgtttag cacaggctat gaataaaata ggtgctaaat
caaattcagg tgaaggtggt 2700gaggatgaag aacgctatga aatcaaagag
ggtgtggata aaaactcagc cattaagcaa 2760gtagcaagtg ggcgttttgg
cgtggattta aactacttaa gtcatgcaaa agaaattcaa 2820atcaaagtcg
ctcaaggtgc aaaaccaggt gagggcggac aattaatggg ctttaaagtc
2880tatccttgga tagctaaggc tagacattct actgcaggtg tgacgcttat
ttccccacct 2940cctcatcatg atatttattc tattgaggat ttggctcaac
tcatttatga tttaaaacat 3000gcgaacaaag acgctaaaat ttcagtaaaa
cttgtaagcg aaaatggcat aggaacggtt 3060gctgcaggtg tggctaaggc
aggcgcgaat ttaatccttg tttcaggtta tgatggaggt 3120acgggtgcaa
gtcctagaac ttctataccg catgctggaa ttccttggga gttaggctta
3180gctgaaacgc atcaaacttt gatcttaaat aagcttagag atagggtaag
attagaaact 3240gatggaaagc ttatgaatgg gcgtgattta gccatagcag
cacttttagg agctgaagaa 3300tttggttttg caaccgctcc tttgattgtt
ttaggttgta caatgatgag agtttgtcat 3360ctaaatacct gtccttttgg
tatagccact caagatacag aacttagaga tcgtttcaaa 3420ggcaaagtag
atgatgtgat taatttcatg tattttatag ctgaggagct tagagaatac
3480atggcaaggc ttggttttga acgcctagat gatatgatag gtcgcgtgga
taaactccgc 3540caaaaaagtg tgcaaggtaa agcaggaaag ctaaatttag
ataaaatttt aaaatccttg 3600cctacttata atagaaccgc tgtgcatttt
aaagattata aagacaataa acttgaaaaa 3660acgattgatt atagaatttt
actaccactt tgtaaaaatg ctgtggagaa aaaagagcct 3720atcaaacttt
ctttagaagt aggaaatcaa agtcgtactt ttgcaactat gctttcaagt
3780gaaattttaa aaacttatgg aaaagatgct ttagatgaag atagtatcca
tatcaaagct 3840ataggcaatg caggcaatag ctttggagcg tttttgttaa
agggtattaa acttgagatt 3900ataggcgata gcaatgatta tttaggcaag
ggtttaagtg gtggtaaaat catagcaaaa 3960atttcaaatg aagccacttt
ttcacctgaa gaaaatatca tcgcaggaaa tgcttgttta 4020tacggagcta
caaaaggtga agtgtattta gatggtatag caggggaaag attttgtgta
4080agaaattcag gggctttggc tgtggtttta ggaacaggcg tgcatggttg
tgaatatatg 4140acaggtggac aagttgtagt tcttggagat gtgggtgcga
atttcgctgc tggtatgagt 4200gggggcgttg tttatatctt tggaagacac
aatgaagctc atgtaaatac cgagcttgtg 4260gatattaaag atcttaatgc
taaggatgaa aaagaattaa aagctgtgat agaaaaacat 4320atcacttata
cagattctaa aaaggctaaa gatattttag aaaaattcga taaaaaagac
4380ttttttaaag tcatgccaag agattatgaa aagatgttaa aaatgcttga
tctttgtaaa 4440aatgaaaaag atccaaattt agcagcattt ttgaaaatca
cacaaaaata a 4491604554DNACampylobacter jejuni 60atgagtaaat
ttaaagtaat agaaattaaa gaagatgcaa gacctagaga ttttgaagca 60tttcaactaa
gacttgcaag tcctgaaaaa atcaaatcat ggtcttatgg agaagtaaaa
120aaaccagaaa ctattaatta tagaacctta aagcctgaaa gagatgggct
tttttgtgca 180aagatttttg gacctataag agattatgag tgtctttgtg
gtaaatacaa aaaaatgcgt 240tttaaaggcg ttaagtgtga aaaatgtggt
gttgaagtag caaattcaaa agtgcgtcgt 300tctagaatgg ggcatatcga
gcttgtaact cctgtggctc atatttggta tgttaattct 360cttccaagcc
gtataggaac gcttttgggt gttaagatga aagatcttga gcgcgtgctt
420tattatgaag cttatattgt tgaaaaccca ggtgatgctt tctatgataa
tgaaagtact 480aaaaaagtgg aatattgcga tgttttgaac gaagagcaat
atcaaaattt aatgcaacgc 540tatgaaaata gcggctttaa agcaagaatg
ggtggagaag ttgttcgtga tttacttgca 600aatttagatc ttgtagcgct
tttaaatcag cttaaagaag aaatgggggc tacaaattca 660gaggctaaga
aaaaaactat cattaaacgc ttaaaagttg tagaaaattt cttaaattca
720aatttaaacg ccaataccga tagcgacgaa gctgtaccaa atcgtcctga
atggatgatg 780attacaaatc ttccagtttt accgccagat ttgcgtcctt
tagtggcttt agatggtggg 840aaatttgcag tttctgatgt gaatgactta
tatcgtcgtg ttatcaatag aaatacacgt 900cttaaaaaac ttatggaact
tgatgcacct gaaatcatta tcagaaatga aaaaagaatg 960cttcaagagg
ctgttgatgc gctttttgac aacggtcgtc gtgctaatgc tgtaaaagga
1020gcaaataaac gcccattgaa atctttaagt gaaatcatca aagggaaaca
aggacgtttt 1080agacaaaatc tactaggtaa aagggtggat ttctcaggtc
gtagcgttat tgttgtaggt 1140ccaaaactta gaatggatca atgcggttta
cctaaaaaaa tggctttaga gctttttaag 1200ccacatcttt tagctaagct
tgaagaaaaa ggttatgcaa ccacagtaaa acaagctaaa 1260aaaatgatag
aaaataaaac caatgaagtt tgggaatgtt tagaagaggt agtaaaggga
1320catcctgtta tgctaaaccg tgcacctact ttgcataagc tttctatcca
agcttttcat 1380cctgttttag tggaaggtaa ggctatacaa cttcatcctt
tggtttgtgc agcatttaac 1440gcagactttg atggggatca aatggcagtg
catgtaccgc tttctcaaga ggctattgca 1500gaatgtaaag tgcttatgct
ttcatcaatg aatattcttt taccagcaag cggtaagtct 1560gtaaccgttc
catcgcaaga tatggtttta ggaatttatt atctttcgtt ggaaaaagca
1620ggtgctaaag gttcgcataa aatttgtaca ggcattgatg aagtgatgat
ggcacttgaa 1680agcaagtgtt tggatattca tgcgagcata caaactatgg
tagatggtag aaagattacc 1740actacagcag gaagattgat tgttaaatcc
atcttgcctg attttgtgcc tgaaaatagt 1800tggaataaag tcttaaagaa
aaaagacatt gctgcgcttg tagattatgt ttataaacaa 1860ggtggtttag
agattacagc aagtttctta gatagactta aaaatttagg ttttgaatat
1920gcaactaaag caggtatttc aatttcgatt gcagatatta ttgttcctaa
tgataaacaa 1980aaagctatcg atgaagcaaa aaaacaagta agagaaattc
aaaattctta taatctcggt 2040ttgattactt caggggaaag atacaataaa
atcattgata tttggaaaag tacaaacaat 2100gttctttcaa aagaaatgat
gaagcttgta gaaaaagata aagaaggttt taactctatt 2160tatatgatgg
cagattctgg tgctaggggt agtgcagctc aaatttctca gcttgctgcg
2220atgagaggac ttatgaccaa acctgatggt tctattatcg aaacgcctat
tatttcaaat 2280ttccgtgaag ggctaaatgt tcttgaatac tttatttcaa
ctcacggtgc tagaaaaggt 2340cttgcagata ccgctcttaa aacagcaaat
gcgggttatt tgacaagaaa actcatcgat 2400gttgcacaaa atgtaaaaat
taccattgaa gattgtggaa cacatgaggg tgttgaaatc 2460aatgaaatta
ccgcagatag ttctattata gaaactttag aagaaagaat tttaggcagg
2520gttttagctg aggatgtgat tgatcctatt acaaattctg tgctttttgc
ggaaggtact 2580ttaatggatg aggaaaaagc aaaaattctt ggcgaaagcg
gtataaaaag tgtcaatatc 2640cgcactccta ttacctgcaa agctaaaaaa
ggaatttgtg caaaatgtta tggtatcaat 2700cttggtgaag gtaaattagt
aaaaccaggc gaagcagtgg gaattatttc cgctcaatct 2760atcggtgaac
caggaacgca gctaactcta agaactttcc atagcggggg aactgcaagt
2820acggatttac aagatagaca agtaagcgca caaaaagaag gttttataag
attttataat 2880cttaaaactt ataaaaacaa agaaggtaaa aatatcgtag
caaatcgtag aaatgcggcg 2940gttttacttg tggagccaaa aatcaaaact
ccatttaaag gtgtgattaa tatagaaaat 3000attcatgaag atgtgattgt
ttctattaaa gataaaaaac aagaagtaaa atacatatta 3060agaaaatacg
atcttgctaa accaaatgaa ttagcaggtg taagtggcag tatagatgga
3120aaactttatt tgccatatca aagcggcatg caagtagaag aaaatgaaag
tatcgtagaa 3180gtgattaaag agggttggaa tgtaccaaat cgtattcctt
ttgcgagtga aattttagta 3240gaagatggcg agcctgtagt tcaaaatatc
aaagcaggcg aaaaaggaac actcaaattt 3300tacatcctta aaggcgatgg
tttagataga gtaaaaaatg ttaaaaaagg tgatattgtt 3360aaagaaaaag
gattctttgt agtgattgct gatgaaaatg atagagaagc aaaaagacac
3420tatatcccaa gagaatctaa gatagaattt aacgatagtg aaaaaatcga
tgacgcaaat 3480actatcattg caagtgctcc taaaaaagaa agaaaagtga
ttgcagaatg ggatgcttat 3540aataatacta tcattgcaga aattgatggt
gttgtaagct ttgaggatat tgaagcaggt 3600tatagtgccg atgagcaaat
tgatgaagct acaggtaagc gttctttagt aattaatgag 3660tatttaccta
gcggagttag accaactttg gtaattgcag gaaaaggtga taaagctgtg
3720cgttatcacc ttgaaccaaa aaccgttatt tttgttcatg atggcgataa
aattgctcaa 3780gcagatattt tagcaaaaac tccaaaagca gcagctaaat
caaaggatat tacaggaggt 3840cttccaagag tttctgaact ttttgaagca
agaaaaccaa aaaatgcggc tgtgattgca 3900gaaattgatg gtgttgttcg
ttttgataag cctttgcgtt ctaaagaaag aattattatc 3960caagcagaag
atggaacaag tgctgagtat ttaatcgaca aatcaaaaca tattcaagta
4020agagatggag agtttattca tgcaggtgaa aaacttacag atggagttgt
ttcgagtcat 4080gatgtgctta aaattttagg tgaaaaagcc ttgcattatt
atttgatttc tgaaattcag 4140caagtttatc gcggacaagg tgttgtgatt
tctgataaac atatagaagt tatcgtttct 4200caaatgctaa gacaagtaaa
agttgtagat agtggacata cgaaatttat tgaaggtgat 4260ttggtttcaa
gacgtaaatt ccgtgaagaa aatgaaagaa tcattagaat gggtggagaa
4320ccagctattg ccgagcctgt gcttttaggt gtaacaagag cagcgattgg
aagtgatagt 4380gtgatttctg cggcttcatt ccaagaaact accaaagttt
taactgaagc aagtatagca 4440ggtaaatttg actacttaga agatttaaaa
gaaaatgtta ttctaggtag aatgattcct 4500gttggaacag ggctttatgg
cgaacaaaat ttaaaactta aagaacaaga ataa 455461156RNACampylobacter
jejuni 61auggcuuaug aagaugaaga agauuuaaau uacgaugauu augaaaacga
agaugaagaa 60uauccacaaa aucaccauaa aaauuauaau uacgaugaug augauuauga
auacgaugau 120gauaacaaug augaugauuu uuaugagaug gauuaa
15662198RNACampylobacter jejuni 62augaucaauc cuauacaaca aaguuaugug
gcaaauaccg cauuaaauac aaauagaaua 60gauaaagaaa cuaaaacaaa cgauacucaa
aaaacggaaa augauaaagc gaguaaaauc 120gcagagcaga uuaaaaacgg
uacuuauaaa aucgauacaa aagcuacagc ugcugcgauu 180gcugacucuu uaaucuaa
19863351RNACampylobacter jejuni 63augaaaaaaa uacuuauucu
acuuacacua
ugugcuuuug cuuuuggugc aagugaaugu 60gauagaaaaa ucgaucguau caauaaagaa
aucaguuuuu cuaaagcgca uaaugauaca 120gcuagaacuu uaagcuuaga
gcuugcuuua aaacaaguac aaaaugauug ugcuaaagau 180ccuauguuuu
augauaaaaa guuagaagcu aaaaaacuua aagaacaaga aguggaaaaa
240aucgaaaaag aacuugaugc uuuaaaagaa caaaaagauu auaugagcaa
ggcugaguau 300aaagcuaaaa aagaagcuuu aaaagaacaa aaagagaaaa
ucaaaaaaua a 35164417RNACampylobacter jejuni 64augaguuuug
gugaaauuau aguuauuuua guuguagcga uuuuagucuu aggaccugau 60aaacuuccag
aagcuauagu acaaauagca aaaauucuaa aggcuguaaa acgcaacaua
120gaugaugcaa aaucaagcau agaaaaagaa auacgcauca augacuuaaa
agaagaagcu 180aagaaauaca aagaugaauu uucaagcacu aaugaaaaua
uacgcaaaaa acucagcuuu 240gaagaauuug augaccuuaa gagagauauu
uuagauaaaa caaagguaga uuuaaccuuu 300gauagcagag augauaaagu
aaaaaauaac cuuagcggac aaaauuuaaa uacagaagaa 360aaaccaaauc
uuagcaaauu agaaacacaa gauaaaaacg gaaaaauaaa uguuuga
41765426RNACampylobacter jejuni 65augcaaaaag cuaaaauuuu aauugccuua
aguuuuuuuu uauuaguuuu aucugccugu 60ucuaaugaug aaaaaaauau uuccaagacu
caaaauacag aucaagaagu aguccaaaua 120gaacaaaacg augaaaaaac
agaauuaagu gacuccaacc ugccuuuacc uguagaugau 180gaagcacaaa
guucaaauga ugaacaugaa gucaauccua guauuaucaa cucuuuauau
240aaacaaaaau gcgccacuug ucauggagaa aaaggggaau uaaaaccuaa
aaacagcacg 300gccauuaaaa ccuugagcaa uaaaauuuuu auacaaaaaa
uaaaaacgau aaaagauaaa 360aaccauaguu uuuuaaguga ugaacaaauu
caaaauuuag cugauuuuau aaacaaagga 420aaauaa 42666435RNACampylobacter
jejuni 66augcgaagac uaaguauuuu acuugcaauu uuaauaguua uuaauauaac
agcuugugau 60aguaaaacag aaaauuacua caaaaaucuu ccuagcgaag caaaagaaaa
agcgaaagaa 120ugcaaagaaa gcggaacucu uagcgaagau uguauuaaug
cauuaaaagu ugguguuaaa 180ccaacaaaug aagaggguaa auacagucca
aacacaccga aaaaaucuga uaaucaaaua 240uuagaagcuu uaaaacaaaa
ugauuuaaaa aaagaaaaaa ccacaaaaga uauuaaccaa 300aguucagaaa
auaaugaaag uauuauaaua ccaccuauag cagaaacacc uucugaaauu
360uauccuucca aaacaacaga aaacaaucaa aguucuaucu uuagcgauga
uguuaauaug 420acacaggaaa aauga 43567525RNACampylobacter jejuni
67augaugaaga agaaauuggu uuuauuagga agugcugcag ugguauuuuu ugccgcuugu
60gcaaugaaua gugggguaag uucagaacaa auuggacuua gaaaagcaag uuuagaaaau
120gaaaauaaag uaaauuuagu ggaggcaaau uucacaacuu uacagccugg
ggaaucuacu 180cguuuugagc guucuuauga aaaugcacca ccauuaauuc
cgcaugcuau ugaagauuug 240uuaccuauaa cuaaagauaa caauaugugc
uuaagcugcc augauaaggc uauagcagca 300gaugcuggug caacuccacu
uccugcuagu cauuauuaug auuuuagaca caauaaaacc 360acaggagaua
ugauuagcga uagucguuuu aauugcacuc agugucaugu uccacaaagu
420gaugcaaaac cuuuaguggg aaauagcuuu aaaccugaau uuaaaaauga
acaauuaaaa 480agucguucaa auuuaauuga ugugauuaau gaggguguaa aguaa
52568606RNACampylobacter jejuni 68augaaaaaaa ucaaaaaaau cauucaaauu
gguaugauag gugguuuagc agcuguugcu 60ggaggugcuu uagcagguug uggaaguaau
aaugacaaug cagauacuuu aaaucaagcg 120gcuaaugcuc aaggagcuuu
uguaauuauc gaagaaacag cuccagggca guauaaaauc 180aaagaucaau
auccaaguga ugaaacaagg guaguuuuaa aagaucuuaa ugguacagaa
240agaauuuuau ccaaagaaga aauggaugcu uugauuaaag aagaagcagc
uaaaauugau 300aauggaacuu caaauuuaac uaaagacaau ggacaaauca
guaguggggg auugaguuua 360ggggaaacuu uacucgcaag ugcugcaggu
gcuauuuuag gaaguuggau agguucaaaa 420cuuuucaaua aucaaaauuu
ugccaaccaa caacgcggug cuuuuucaaa ucaaagugcu 480uaucaaagga
guguuaauag uuuuaauaaa gcaggcacaa caagcucugc uucaagugcu
540aaaaaaucag guuuuuuugg uggaggcucu aaagccacaa guucuaguuc
uucuuuuggc 600ucuuaa 60669633RNACampylobacter jejuni 69augacaaaau
uuuuaagcau uuguaguuua auugcgaugu uguuaagugg uuguggaagu 60gauuuuccug
gacaaccuag cgauguggcu agaguucagc aaaacaaaua uccaaaugga
120aauuuaaaaa aagaaauucc cuauaauaaa gauucaagaa uacauggguu
aaaaagagcu 180uuuuaugaca auggucagcu aagagcugaa gaaaauuaua
aaaauggaaa aaaagaugga 240auuagcaggg aauauucuag aaaugggcaa
uuacuugagg aggugcauuu uaaagauaau 300cgcggauaug gugauuuugc
aagcuauuau gagaauggaa auaugagagc aaagggaaaa 360cuacuuggcu
auaaugaaga ugguaugcca gaauuugaag guaauuacaa ggaauauuau
420gaaaauggaa cuuuaaugug ugauuauaau uuugauaaua aagguaaauu
ugauggagua 480caaaagcguu augaugaaaa uggcgccuua gaagacgaag
aaaauuauaa aaauggacuu 540aaaaauggag ucuuuagaga auauaaaaaa
ggggagauug uaagagaaga agaauauaaa 600aaugguauuu uaguagcaaa
accuaaaaau uag 63370735RNACampylobacter jejuni 70augaaaaaaa
uauuuuuaag uguuuuuuug guuuugaguu uaaaugcuca aaaucuugaa 60auagauaaaa
uaagaacaga uuuguauucu aaaaguggag caaauguucu uaaaaaaguu
120gaaauuucuu uggaauuuga ugggaauaau uuaaaagaaa augaaaauaa
guuaauugau 180gcuguaaaua caguaauuuc aggguuuuuu uaugaagaua
uuuuuacaga aauuggaaaa 240aauaauuuua aaaaaacuuu agaaaaauuu
uuagauaaga aauauaagau uaaacuagau 300gauauauaua ucauaucuuu
aaguggagug gaaaaauuug auuuagagga guuuaaacgc 360uuuuuagaaa
guacugaggc uaaagagaag gguaugggua gcgagguaaa aaaagcacuu
420gaaaacuuag aaguuccuaa aacucaaguu ccuaguguug aaaagauccc
aacuccuagu 480guuccaaauu uagaagucaa gcaaguugaa cagcuuuuca
aagauccaga ugaagaaaau 540aaaaaugaca auggagaaau caauauagau
aauuuaaaua caccuaaaau gacuccagau 600auagaagaaa aaauuaaaag
agauuuaauc gccaauccuc cacaaauauu caaagaaaau 660aacgcaagca
agccuuauca uuugccacaa acaggcuaug auauaaagcu ugaugagaau
720ucaacgcaaa auuag 73571780RNACampylobacter jejuni 71augaaaaauu
augguuugag uaauuuaaau ucguuuuuac uugcuuuagc aauauauauu 60aguauaguaa
uucuuguuuu uuuuagacuu guaagcgagg uugaaccugc uauacaauau
120acugauauaa aagauaguuu uguagauauu gaacuugcug aaccaucaaa
acaaguuauu 180acucaaagca acacuccuaa agaaauacaa aaaccaacag
agcaaauuga uauagaaaag 240cuuuuugcuc agacuacaaa uaaaacaguu
aaaacugaag auauugacca aaaggcaagu 300aauuuuaaug agcuuuuugg
aaauauuaaa gaaauacaag aagaaaagac uacaaaaauu 360caaucaagug
cuaaaucagg aauuucuagu gccccaaaac cucaagcuuc ugaacuugua
420aaacaacuua augauaguuu acuucaagaa gaaaguucaa cgcaaggcga
aagcacaaag 480gcgcaaaaga uuggaauuua ugaugaauuu uuagggaaag
uugugcguau uaucacucaa 540agauggacuc aguauuaucc aaauagugaa
aaaauuucug uuaagguaaa aauuuuuauu 600gaugaaaaug gaaaauuugg
cuauacuuca guugaaaaaa gugggaaucc uuuauaugau 660gcuaaagugg
cugaauuuuu agaaagucaa aaagguaaau uuauuacuua uccuccgcaa
720aauaaaaaua uaagcauuac caugaauuua agagaugaag uaaaaguuaa
aaaugauuag 78072822RNACampylobacter jejuni 72augaaaaaau uuucuuuagu
cgcagcaacu uugauugcag guguaguuuu aaauguaaau 60gcagcuacag uagcuacugu
uaauggcaag agcauuagcg auacagaagu aagugaauuu 120uuugccccua
ugcuuagagg acaggauuuu aaaacuuugc cagauaauca aaaaaaagcu
180cuuauucagc aauauauuau gcaagauuua auuuugcaag augcuaaaaa
acaaaauuua 240gaaaaagacc cuuuauacac aaaagaacuu gaucgugcaa
aagaugcaau acuuguuaau 300guuuaucaag agaaaauuuu aaauacuauu
aaaauugaug cggcuaaagu uaaagcuuuu 360uaugaucaaa auaaagacaa
auauguaaaa ccugcaagag ugcaagcaaa acauaucuua 420guagcgacag
aaaaagaagc uaaggauauu auuaacgaac uuaaagguuu aaaagguaaa
480gaacuagaug cuaaauuuag cgagcuugcu aaagagaaau caauugaucc
agguucaaaa 540aaccaaggug gugagcuugg uugguuugau caaucaacua
ugguaaagcc uuuuacagau 600gcugcuuucg cgcuuaaaaa ugguacuauu
acuacaacuc cgguaaaaac gaauuuuggu 660uaucauguaa ucuuaaaaga
aaauucgcaa gcuaaagguc aaaucaaauu ugaugaagua 720aaacaaggua
uugaaaacgg acuuaaauuu gaagaauuua aaaaaguuau caaucaaaaa
780ggccaagauc ucuuaaauag ugcuaaagug gaauauaaau aa
82273837RNACampylobacter jejuni 73auggaaaauc aaaaaaauga auuugaugau
auuauuuuag aaaaaaguaa uaaaagugaa 60aaaguaaaaa aaauucuuuu acgaguuauu
gcuuuaguua uuuuguuuuu agcuaucaug 120auaguuauga agcuuauuaa
ugguaguggu gaugaaaaua cgcaaaauca aaguguauug 180ccaagugaac
cuauagcaac ucaagacaau aacaaugaua cuucuuuuga aaguaugcca
240auuacagaua auacuucagc agaagaucaa uuugaggcau uaagaaaaca
auuucaagau 300gaacaaaaua caacucaaaa uacaacaacc ucuaguucaa
auaacaauga uacuacaaau 360uuugcuaugc cugaucaaga aguuccagca
gaaccaacag caacuacuuc agcaaauacc 420acuccacaag caaguacucc
uaaacaagaa guaacacaaa cugcaaaauc uaaagaagaa 480gcaaaaaaac
aaacagcugu aaaaaaagaa aaagaaagug caaaacaaac cccuaaaaaa
540gaacaaaaug caaaugauuu auuuaaaaau guugaugcua aaccuguaca
uccaaguggu 600uuagcaucgg guauuuaugu gcaaauuuuc ucaguaagua
auuuggauca aaaaucaaaa 660gaacuugcuu cuguaaagca aaaagguuau
gauuauaaac uuuauaaaac uacaguugga 720aguaaagaaa uuaccaaggu
uuuaauagga ccauuugaaa aggcagauau ugcagcagaa 780cuugcuaaaa
uccguaagga uauugcaaaa gaugcuuuuu cuuuuacuuu aaaauga
837741173RNACampylobacter jejuni 74augaaaaaga agauuguuuu aaucauuuua
auugcaauac uuggaagugu uggggcuuau 60uuuauuuuuu uuaauaauga ugaaaaaauc
agcuauuuaa cucaaaaaau acaaaaaaaa 120gauauaucuc aaaccauaga
ggcaguagga aagguauaug ccaaagauca agucgaugug 180ggugcucaag
uuaguggaca aauuauaaaa cuuuauguug augugggaac ucauguaaag
240caaggugauu ugaucgcuca aauugauaaa gauaaacaac aaaacgauuu
agauauuaca 300aaagcucagc uugaaagugc uaaggcuaau uuagagagua
aaaaaguugc ccuugagauu 360gcgaauaagc aauaucaaag agagcaaaaa
cuuuaugcag cuaaagcaag uucucuugaa 420aauuuagaaa cucaaaaaaa
uaauuauuau acuuuaaaag ccaauguugc agagcuuaau 480gcucaaguag
uucagcuuga aaucacucuu aaaaaugcac aaaaagauuu agguuauaca
540accauuacug cuccuaugga ugguguugug auuaauguag cuguagauga
aggacaaaca 600guuaaugcua aucaaaacac uccuacuaua guccguauag
cuaauuuaga cgaaauggaa 660guaagaaugg aaauagcaga ggcugaugug
aguaagauaa aaguaggaac agagcuugau 720uuuucuuugc uuaaugaucc
ucaaaaaacu uaucaugcua agauugcaag uauagaucca 780gcugauacug
aagugaguga uucuaguaca agcucaagcu caucaaguuc gaguucuuca
840agcucaagcu caucaaaugc uauuuauuau uacgcaaaau uuuauguagc
caauaaagau 900gauuuuuugc guauugguau gaguauacaa aaugaaauug
ucguagcaag ugcaaaggcu 960guuuuagcag ugccaacuua ugcaauuaaa
agcgauccaa aaggcuauua uguugaaauu 1020uuggaaaauc aaaaagcugu
uaaaaaauau gucaaacuug gcauuaaaga uucuaucaau 1080acucaaauuu
uagaaggugu aaaugaagau gaagaauuga uaguaagcuc aagugcugau
1140gguuuagcuc cuaaaaugaa guugagauuu uaa 1173751629RNACampylobacter
jejuni 75augaaaauuu uacuuuuaaa ugaaaacccu guaguuucaa gacuuguaag
ccuuagugcu 60aaaaaaaugu cuuaugauuu ugaagaacuu aaugcuuaua gugaaaauuu
ggguaauuau 120gaugugauug uuguagauag ugauacucca gcaccuuuaa
aaauucuuaa agaaaaaugc 180gauagguuga uuuuuuuagc cccgcgaaau
caaaauguag aagauauaga ugcgcaaauu 240uuacaaaaac cuuuuuuacc
uacagauuuu uuaaauuuac uuaauaauaa agaugcgaac 300aagcauacau
cuauugauuu gccaauguua agcaaugaug aaaauccuua ugcugauaua
360agcuuggauu uagauaauuu aaauuuagau gauuugccug augaaaauuc
uuuagauaua 420aauucagagg gaauggaaga uuugaguuuu gaugaugaug
cucaagauga uaaugcaaac 480aaaacuuuag aaacucaaaa uuuagaacau
gaaacaauua aagaacagac ucaagaagac 540acucaaauug auuuagauuu
aacuuuagaa gauggcgaaa gugaaaaaga agacuuaagc 600caagaacaua
cagcuuugga uacugagccu aguuuagaug agcuagauga uaaaaaugau
660gaagauuuag aaaucaaaga agaugauaaa aaugaagaaa uagaaaagca
agaauuauua 720gacgauucua aaacaaauac auuagaaaug caagaagagc
uuagcgaauc ucaagaugau 780aauucaaaca aaacuuuaga aacucaaaau
uuagaacaug acaauuuaga acaagaaaca 840auuaaagaac agacucaaga
agacacucaa auugauuuag auuuaacuuu agaagauggc 900gaaagugaaa
aagaagacuu aagccaagaa cauacagcuu uggauacuga gccuaguuua
960gaugagcuag augauaaaaa ugaugaagau uuagaagaua auaaagaauu
acaagcuaau 1020auaagcgauu uugaugaucu uccugagguu gaagagcaag
aaaaagaaau ggauuuugau 1080gaucuuccug aggaugcuga auuuuuaggu
caagcaaaau auaaugaaga aucagaggaa 1140aauuuagagg aguuugcucc
uguuguggaa gaagauauuc aagaugaaau agaugauuuu 1200gcuucaaauu
ugaguacuca agaucaaauc aaagaagaac uagcucaacu ugaugagcuu
1260gauuauggua uugauaguga caauaguagc aaggucuuag aagauuuuaa
agaugaacca 1320auuuuagacg auaaagaauu aggaacaaau gaagaggaag
ugguugugcc aaauuuaaau 1380auaagugauu uugauacauu gaaagaaagu
gauauacaag aagcucuugg agaggaaauc 1440uuagaaaaaa augaagagcc
uauaguaagu gauguaacua aagaugauaa uagcgaagag 1500auaguuaaug
agcuuagcca aagcauagca ggagcgauca cuucaaguau uaaagaugau
1560accuuaaaag cugcucuuaa ggguaugaau augaauauaa auauaaacau
uaguuuuaaa 1620gaggauuga 1629761833RNACampylobacter jejuni
76augaaaaaaa auauuuuacg cuuagguauu gucguuuugg uuuuacuuau agcgggaguu
60uuauggcuaa auaaugauau caaucaaaaa aaagaagaug aagcaaauaa aaaugccauu
120gcagcaaaug cugauuuuuc uuugcuuagc gaugaugauc caaauuuuga
aaaauggggu 180aagguuuuuc cagagcaacu aaaaauguau uuaacgguug
aaaaagaaga gccuaaggcu 240acugaauuug gugguaauuu agcuuauuca
aaauuaauuc gcuuuccuca auuaaccaua 300cuuugggcag gauauccuuu
uagucuugau uucaaugaag aaagagggca uuuuuggguu 360caaguagauc
aaaugaaaac agcaagaaac aacaaagauu uucuuaaugc ccauggacuu
420gcugcuuuua aaggucaacc ugcagcuugu augaauuguc auaguggaug
gacuccaugg 480cuuauaaaaa auguugcuaa aggagauuuu acugcuuuua
acucuacaaa uuauuggacu 540augauuaaaa auaucccagc uguugauggu
auaguagaaa auucaccuga acaugcaggc 600ccacauggug guaaaagaau
ggguguaacu ugugcagauu gucacaaucc aaaugauaug 660aguuuaagac
uuacucgucc agcagcuauu aaugcuuuag uuucuagagg auaugaaaaa
720gauccaguac aaggcguaaa agcuacaaga gaagaaauga gaacuuuagu
uugcucucaa 780ugucauguug aauacuauuu uaaaccaaca ggggaaaaag
uaaaaguaau gggugaaacu 840auuguagaug auaguucuaa aaaauggugg
aauggaacuc agaaaaauua ugaugaguau 900gaguuuugga gagauggcaa
uaaaguuaaa gagauugaaa ccgaugguau aguuuuaacu 960uuuccuugga
gugaguggaa aaaaggacaa ccauuuagaa uugaaaugcu agaugauuau
1020uaugauaaag uucguggagu auuuggagcu gauuuuacuc auaaauuaac
aggagcgcaa 1080auuauuaaaa uucaacaucc agaaagugaa cuuuauagug
gcggugugca ugcugcaaau 1140ggaguaaguu gcguggauug ucauaugccu
uauguuagag aaggagcuaa aaagguuacu 1200caacacaaua ucacuucucc
uuuaagagau auuaacucug cuuguaaguc uugucauaaa 1260caaagugaag
auuauuuaaa agcucaagug cuugacauac aaaacagcgu ugcacaugau
1320caaagaacug cagaguaugc aauuguuagu uugauuaugg auacuaaaaa
auuacgcgau 1380gaacuaggca auauggaaaa auuccaaagc gauggaaaag
cugaugcgaa aaaaauuagc 1440gaagagcuaa aagaaguuuu agaacuucau
cguaaagcuc aaaugagagc ggauuuuguu 1500aaugcugaaa acucaacugg
uuuccauaau ccucgcgaag cuucaagaau guuauugcaa 1560gcuguugaua
uggcaagaau gggacaaacu aaacuuguag aaauugcagc ggcaaaugga
1620auuaaagauu uuaaaacuuc aaauuuaggu uuugaagaua uucaaaaauu
uaauccagga 1680gagcuuuauu auaaaguaga uguuaauaau cauaaagcug
gagagcguua cuaugcagau 1740gaaaaagaug uuaauggcaa uccuccaaaa
gaacuuuuag agcaugauaa agagcuugcu 1800ccuuauaauu aucaagugau
ugauaaaaaa uaa 1833771980RNACampylobacter jejuni 77augaaaagcg
uaaaauugaa gguuucgcug auugcaaauu uaaucgcagu aguguguuug 60auaauuuuag
guguuguaac auuuauauuu guaaagcaag caauuuuuca ugaaguugug
120aaugcugaaa uaaauuaugu uaaaacggcu aaaaauucua uagagucuuu
uaaggcaaga 180aauucuuuag cucuugaaag uuuagcuaaa aguauuuuaa
agcauccuau agaacaguua 240gauagucaag augcuuuaau gcauuauguu
ggaaaagauu uaaagaauuu uagagaugcu 300ggaagauucu uagcaguuua
uauugcucaa ccaaauggcg aacuuguugu aagcgaucca 360gacucugaug
cuaaaaauuu agauuuugga acuuauggaa aagcugauaa uuaugaugcu
420agaacaagag aguauuauau agaagcaguu aaaacaaaua aacuuuauau
uaccccaucu 480uauauugaug uaacuacaaa uuuaccuugc uuuacauauu
cuauuccgcu uuauaaagau 540gguaaauuua uagggguuuu ggcuguagau
auucuugcgg cagauuugca agcugaauuu 600gaaaauuuac cagguagaac
uuuuguauuu gaugaagaaa auaaaguauu uguuucuaca 660gacaaagcuc
uuuuacaaaa agguuaugau auuagugcaa uugcaaaucu ugcuaaaacu
720aaagaggauc uugaaccuuu ugaguauacu agaccaaaag augguaauga
aagauuugcu 780guaugcacaa agguuucugg aauuuauacu gcuugcguug
gagagccaau agaacaaaua 840gaagcuccag uuuauaaaau ugcauuuaua
caaacugcga uuguuauuuu uacaaguauu 900auuagcguca uccuccuuua
uuucaucgua ucaaaauacc ucuccccacu ugcagcuauc 960caaacagguu
uaacuucauu cuuugauuuu aucaacuaua aaacaaaaaa uguuuccacu
1020auagaaguaa aaagcaauga ugaauuugga caaaucucaa augcuaucaa
ugaaaacauu 1080cuugcuacua aaagaggcuu agaacaagac aaucaagccg
uuaaagaauc aguucaaacc 1140guaucaguug uagaaggugg uaauuuaaca
gcaagaauua cugcuaaucc aagaaaccca 1200cagcuuauug aacuuaaaaa
uguucuaaau aaacuucuug auguuuuaca agcuagagua 1260gguucugaua
ugaaugcuau ucauaaaauu uuugaagaau acaaaagcuu agacuuuaga
1320aauaaauuag aaaaugcuag cgguagugua gaauuaacua cuaaugcuuu
aggugaugaa 1380auaguuaaaa ugcuaaaaca aaguucagac uuugcuaaug
cuuuagcuaa ugaaagugga 1440aaauuacaaa cugcuguuca aagcuuaacc
acuucuucaa auucucaagc ucaaucuuua 1500gaagaaacug cagcagcuuu
agaagagauc acuucuucua ugcaaaaugu uucaguuaaa 1560acuagugaug
uuaucacuca aucugaagag auuaaaaaug uuacagguau uauaggugau
1620auugcagauc aaaucaaucu uuuagcuuua aaugcagcua uugaagcagc
ucgugcugga 1680gaacauggua gaggcuuugc agugguagcu gaugaaguua
gaaaguuagc ugaaagaacu 1740caaaagucuu uaucagaaau ugaagcuaau
acuaauuuac uuguucaauc uaucaaugau 1800auggcagaaa guauuaaaga
acaaacugca gguaucacuc aaaucaauga uagcguagcu 1860caaauugauc
aaacuacuaa agauaauguu gaaauugcua augaaucagc uauuauuucu
1920aguacaguaa gugauauagc uaauaauauc uuagaagaug uuaagaagaa
gagguuuuaa 1980781998RNACampylobacter jejuni 78augcaaucaa
uaaauucagg caaauccguu ggaauuucag cuaagcuuac gcuauggguu 60ggaauuuuag
uuguauuaau uuuagcaauc acaagugcua uuaguuacuu ugauucgaga
120aacaauacau augaauugcu aaaagacacu caguuaaaaa cuaugcaaga
uguggaugcu 180uucuuuaaaa gcuaugcuau gucaaaaaga aaugguauuc
aaauacuagc caaugagcua 240acaaaucguc cugauaugag cgaugaagag
cuaaucaauc uuaucaaagu aauuaaaaaa 300guuaaugacu acgaucuagu
uuauguagga uuugauaaua caggaaaaaa uuaucaaucu 360gaugaucaaa
uuuuagaucu aucaaaaggu uaugauacua aaaaucgucc uugguauaaa
420gcugccaaag aagcaaaaaa gcuuauagua acagaaccuu auaaauccgc
cgcuagcgga 480gagguugguu uaacuuacgc ugcuccauuu uaugauagaa
auggaaauuu uagagguguu 540guagguggag auuaugaucu agcaaauuuu
ucaaccaaug uuuuaacugu aggaaaauca 600gacaauaccu uuacugaagu
acuugauuca gaaggaacaa uacuuuuuaa ugaugaaguu 660gcuaaaauac
uaacaaaaac agaauuaagu aucaauaucg ccaaugcaau caaagcaaau
720ccugcucuua uugauccaag aaaccaagau acuuuauuua ccgcuaaaga
ucaccaaggc 780guagauuaug cgauuaugug uaauucugcu uuuaauccuu
uauuuagaau uuguacaaua 840acagaaaaca aaguuuauac cgaagcuguu
aauucuauuu uaaugaaaca aguuauaguu 900gguauuauag cuauaaucau
agcuuuaauc uugauuagau uuuuaaucag cagaagucuc 960uccccacuug
cagcuaucca aacagguuua acuucauucu uugauuuuau caacuauaaa
1020acaaaaaaug uuuccacuau agaaguaaaa agcaaugaug aauuuggaca
aaucucaaau
1080gcuaucaaug aaaacauucu ugcuacuaaa agaggcuuag aacaagacaa
ucaagccguu 1140aaagaaucag uucaaaccgu aucaguugua gaagguggua
auuuaacagc aagaauuacu 1200gcuaauccaa gaaacccaca gcuuauugaa
cuuaaaaaug uucuaaauaa acuucuugau 1260guuuuacaag cuagaguagg
uucugauaug aaugcuauuc auaaaauuuu ugaagaauac 1320aaaagcuuag
acuuuagaaa uaaauuagaa aaugcuagcg guaguguaga auuaacuacu
1380aaugcuuuag gugaugaaau aguuaaaaug cuaaaacaaa guucagacuu
ugcuaaugcu 1440uuagcuaaug aaaguggaaa auuacaaacu gcuguucaaa
gcuuaaccac uucuucaaau 1500ucucaagcuc aaucuuuaga agaaacugca
gcagcuuuag aagagaucac uucuucuaug 1560caaaauguuu caguuaaaac
uagugauguu aucacucaau cugaagagau uaaaaauguu 1620acagguauua
uaggugauau ugcagaucaa aucaaucuuu uagcuuuaaa ugcagcuauu
1680gaagcagcuc gugcuggaga acaugguaga ggcuuugcag ugguagcuga
ugaaguuaga 1740aaguuagcug aaagaacuca aaagucuuua ucagaaauug
aagcuaauac uaauuuacuu 1800guucaaucua ucaaugauau ggcagaaagu
auuaaagaac aaacugcagg uaucacucaa 1860aucaaugaua gcguagcuca
aauugaucaa acuacuaaag auaauguuga aauugcuaau 1920gaaucagcua
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1980aagaagaaga gguuuuaa 1998792253RNACampylobacter jejuni
79augagaauua caaauaaacu uaacuucaca aauaguguga auaauucuau gggcggucaa
60agugcuuuau aucagauauc ucaacaacuu gcuucagguu ugaaaauaca aaauucauau
120gaagaugcaa gcacuuauau agauaauacg cgucuugaau augaaauuaa
aacguuagaa 180caaguaaaag aaucaacaag uagagcucaa gaaaugacuc
aaaauaguau gaaagcuuua 240caagauaugg uuaaacuucu ugaagauuuu
aaaguuaaag uaacccaagc ugcaagcgau 300agcaauucuc aaaccucaag
agaagcuaua gcaaaagaac uagaacguau aaaagaaagc 360auaguucagc
uugcaaauac caguguuaau ggucaguauc uuuuugcggg uucucaaguu
420gcaaacaaac cuuuugauuc uaauggaaau uauuauggag auaaaaauaa
uauuaaugua 480guaacuggag cuggaacuga aagcccauac aauauaccag
guugggauuu auuuuuuaaa 540gcagauggag acuauaaaaa acaaauaagc
acuaauguua guuuuacaga uaaucguugg 600gauuuaaaua aagacccuga
uaaaacuaaa uaucucacag gagauucuaa auggcaacaa 660cuuaucggac
aaaguuaugu aaaagauaau agcuuagaug cugacaaaga cuuugaguau
720gaugauagca aacuagauuu uccuccaaca acucuuuaug uucaagguac
aagacccgau 780ggaacaaguu uuaaaagugc uguacucguc aaacccgaag
auacuuuaga agauguaaug 840gaaaauauug gagcucuuua ugguaauacu
ccaaauaaua aaguaguaga aguaaguaug 900aaugauagug gucaaauuca
aauuacagau cuaaagcaag guaauaauaa acucgauuuu 960caugcuguag
cuuucacacc acaagcugau gauaaaacug aauuaaauaa uauuauccaa
1020gcagcacagg augaaggcau uacaauggaa gauguuacaa acaggguuau
gacugcugca 1080cuaggaaauc ccaauaaugg agauauuaca aauuuaaaua
auccuguaac cauucaaauu 1140aacggacaaa acuuugaaau ugauuuaaaa
caaacugauu uuaucaaaag uaaaaugaca 1200gauacagaug gaaaugcugc
uaauggagcu gauuacgaua auguguauuu ugaaaaaaau 1260ggcaauacug
uuuaugguaa uguuucucaa guuaucaaag gaagcaaugc uuaugccacu
1320gauucaacca aacuuagcga gguaauggca ggagauagcc uaaaugguac
uacuuuaaau 1380uuaaaaguca auuccaaagg uggaaauucu uacgauguua
cuauaaauuu acaaacuuca 1440acuguaagcu auccugaucc uaauaaucca
ggucaaacca uaagcuuucc uauuaugcau 1500acuaauccug caacuggaaa
uagugggguu guuacaggau caaaugauau uacuuauggu 1560caaauuaaug
auauuauagg uauguuugcu gcagauaaaa uuccuacaac aaccauacaa
1620gccaauaaug gucaaauuaa uaaugcagau uauacccaaa uacaacaacu
caugaaagau 1680ucacaagcua cuguugaugu aaguauggau uauaaagguc
guauuagugu uacugauaaa 1740cuuucaucag gaaccaauau agaaauuucu
cuuagugauu cucaaagugg ccaauuucca 1800gcaccuccuu uuaccacaac
uucuacugua caaaaugguc caaauuuuag uuuuagcgcg 1860aauaauucuu
uaacuauaga ugagccaaau guggauauua uuaaagauuu agauucaaug
1920auugaugcug uuuuaaaagg caauaugaga gcagacuccg aaagugaaaa
cccuagaaau 1980acagguaugc aaggagcuuu agaaagacuu gaucauuuag
cagaucaugu uagcaagcuu 2040aauacaacua ugggagcaua ucauaauacu
aucgaaggug uuaauacacg cacaucauuu 2100uuaagcguua auguccaaag
uauaaaauca aaugugauug auguagauua uggugaggca 2160augaugaauu
uaaugcaagu ucaacuugca uaucaagcuu cucuuaaagc uaguacaaca
2220auuucucaac uuagcuuauu aaauuauaug uaa 2253802598RNACampylobacter
jejuni 80augaugagau cacuuugguc uggcguaagc ggacuacaag cacaucaagu
ugcgauggau 60guugaaggua auaacauuuc aaauguuaau accacugguu uuaaauauuc
ucgugcagau 120uuugggacua uguuuagcca aacugugaaa aucgcuacag
cuccaacuga uggaagaggc 180ggaucuaauc cacuucaaau cggucuuggc
guuucaguaa guucuacaac uagaauucau 240ucucaagguu caguucaaac
cacagauaaa aacacugacg uugcuauaaa uggcgauggu 300uuuuuuaugg
uaagcgauga ugguggucuu acaaacuauc uuacaaggag cggggauuuu
360aaacuagaug cuuauggaaa uuuuguuaau aaugcagguu uuguugucca
aggguggaau 420aucaacuggg augaucaaac uauagauagu ucaagaacuc
cacaaaauau uuuuaucgau 480ccagguaugc auaucccugc agcaaaaucu
acugaaguug cuaucaaagc gaauuuaaau 540agugguuuaa auauaggaac
uucaaguaga aaucuuuaug cacuugauuc uguucaugga 600uggaauacua
aaacccaaag agcagaagau gaaaaugaua caggaacuac ucaguuuuau
660acgacuucua agaauucugu agaagugaca gaaaagggug uggaugcggg
aucacuuuuu 720aacgcgaaag gacaaggacu uaaucuuaga gauggacaag
gaauuugggu aucuuaugca 780gaugcaacau auucuaccaa uaaaguagga
guaaaugcuu uugauccaaa uuuacagcaa 840aaucaaacug cugcuuuuug
gggaacagcu aaucaaaaag ugaauuuaga uauaacuuua 900aaugggguua
gaauucaaaa ugcugauauu caaaguauug augaugcuau ugcuuauauc
960aauaccuuua cugcaccaac ggauacaagg gauggaacag guguaaaagc
gguuaaaaau 1020aaggauggua guggaauuga uuuugucaau gauaaugccg
augguacuac agauaauaug 1080aaaaauauca aucuuguggu ugccaauacc
aauacagcag gugagcuuug gaaugcugua 1140uggaauaaca acaaucaaac
auuuacauuu aauaauaaug guaauggaca ggcuggaaca 1200ccgacuauua
auaaaaaugg uucuucuuug uggacagcua caaauauuac auuuacacca
1260caaccuccuc aagcagcuac gaauguucag cuuacuggug gacuaaaugc
acaaauaaua 1320acagcacaua aauauauuua uaguucaaac ccuguggaua
uagguccuau guauaauccu 1380gacgguggac cagcauucca gccuggugcu
aaugcaacua caagaccaac ugaaccaggu 1440ucagcagcuu auugggaugc
uguuaauggu ggacuuuuaa auacuaaugu aagaacuuuu 1500agaaccacag
aagauuuaag agaacuuuua caaagggaug cuagauaugg gguugauuau
1560gauggaagug gaacuuuugc ugcagcugau auuaaucaaa auauaaaagu
aguaguaacg 1620gcagauggac auuuugcuau uuccaaugcu aaugaacaau
caacuguucc accaaaugcu 1680auuaauggug uaggaaaugc cacuacaaca
gauccaaaaa auaugaguuu uaauauaaca 1740gcuuauagua acaaacaagg
aacuguaagu acuaaugaug cuuucacugc uauuuuuaaa 1800gcuuucgaug
guccuuuggu uauaggaaau cagaucaaag aaagcgaaca acuuaagcuu
1860ucugcuuuuu cggcggggcu ugaaauuuau gauucuuuag guucaaaaca
cacuuuagaa 1920gugcaguuug uuaagcaaag uaccacucaa gaugggggua
augaauggca aaugaucauc 1980cguguaccug aaccugcaga gauuaacacu
acaggcgaag gaccaaacaa uaucaucgua 2040ggaacagcua gauuuaacaa
ugacggcucu uuagcuaguu auacaccaag aacgauaaau 2100uucucaccaa
acaauggugc cgcaccaaau caacaaauca aacuuuccuu uggaacaagu
2160ggaagcaaug acggccuugu aagcucaaau ucugcuucaa cucuaacagg
acaggcaacu 2220gaugguuaua cuucagguaa cuuaaaaccu gaugcuaucc
guguggauga uaaagguaau 2280aucuuaggug aauuuacuaa uggcaaaacc
uuugcuguag caaaaaucgc aauggcuuca 2340guggcaaaua acucaggucu
ugaggaaauu gguggaaauc uuuuuaaagu uacugcaaau 2400agugguaaua
ucgugguagg ugaagcagga acaggagguc guggugagau gaaaaccuca
2460gcucuugaaa ugucaaaugu ggauuuaagu cguucuuuaa cagagcuuau
uaucauucaa 2520agagguuauc aagcaaacuc aaaaaccauu ucaacgagug
aucaaaugcu ccaaacucua 2580auccagcuua aacaauaa
2598812616RNACampylobacter jejuni 81auggcaaaga uuagaauuca
ugaaaucgca aaagaauuag guuaugauag uaaggaaauu 60auugaaaagg caaaugaauu
aggacuugga auuaaaacag caucaaaugc uguagaaccu 120gagauugcgg
cagcuauuua ugaguauaua caaacaagag aaauuccaga agcuuuuaag
180aaaaauauca aaacuccuac agcaaaaaag ccuaaaaaag aaaauauaaa
agaacaagaa 240aagcuaaaug aaucugaaaa aaaagaaccu aaaaaagaag
aaaagcuuaa acaagaaguu 300aaaaaagaag aauuaaaaau ugaaaaagaa
aaugcaaaag aggaagaaaa acaagaaauu 360auugaugcuc auaagccaca
aagccuugcu agugcaacuu uagccaaaag acgcggacuu 420guuauuguua
aaaagaaaaa agacgaagaa gaaauucaag uuaaaaaaga agaaguaaaa
480aauucaaaug auauaucuau caauaaugaa gagcgcuuaa guuuaaaaac
uauguuuuca 540aaugcugaug agaguuuaaa gaaaaagaaa aaagaaaaaa
aaucuuuugu ugcgaguaaa 600aaagaaagua ccgaaaaaau gaauuuuuua
gaugaacaug auuuugguga uauuucuuua 660gaugaugaag augagguagu
auuaccugau uuuaguguaa aagaacaaga aaaaccacaa 720aauaucaaua
aaaaacaacc uaauuuuaua agacaagcug uuggaaauuc ugcggguuuu
780ggguuugaag guggaauuca aagaagaagu cguaaaaaac caucuaaaaa
gauugaaaaa 840aaggaaguag aagaaguagg uagcguugcu auuucuaaag
aaauucgugu guaugaauuc 900gcugauaaga uaggaaaaag cacuagugaa
gugauuucaa aacuuuucau gcuuggaaug 960augacaacaa aaaaugauuu
cuuagaugaa gaugcgauug aaauuuuggc ugcugaauuu 1020gguauagaga
ucaauaucau uaacgaggcu gaugaguuug auuauguaaa agacuaugaa
1080gaagagacug augaaaaaga uuuagugacu agagcaccug ugauuaccau
cauggggcau 1140guugaucaug guaaaacuuc uuuguuagau uauauuagaa
aaucacgcgu ugcaaguggu 1200gaagcagggg guauuacuca gcacgugggu
gcuuauaugg uagaaaaaaa cgggcguaaa 1260auuacuuuua ucgauacucc
aggucacgaa gcuuuuacug cuaugcgugc aaggggugca 1320aguaucacug
auauuguaau caucguugua gccgcagaug augggguaaa accacaaacc
1380aaagaagcga uaaaucaugc uaaagcagca ggugugccua uuauuauugc
uauuaauaaa 1440auggauaaag aagcagcaaa ucccgauaug guaaaaacuc
aacucgcaga aauggaaauc 1500augccaguag aauggggcgg aucuuaugaa
uuuguaggag uuucggcuaa aacaggaaug 1560gggauugaag auuugcuuga
aaucgugcuu uuacaagcug auauuuuaga acuuaaagcc 1620aauccaaaaa
guuuugcuaa agcaagcauu auagaaaguu cugugcaaaa agggcguggu
1680gcgguggcua cugucaucgu gcaaaaugga acacuuacug uaggaaguac
cgugguugca 1740ggcgaggcuu auggaaaagu gcgugcgaug agcgaugauc
aagguaaagc cuuaaaagaa 1800auuaaaccag gcgaaugugg gguuaucgua
gggcuuagug aaguagcaga ugcaggugaa 1860auuuuaaucg caguaaaaac
cgauaaagaa gcaagagaau augcuaauaa acgccacgaa 1920uacaaucgcc
aaaaagaacu uagcaaaucc acuaaaguua gcauugauga gcuuggagcu
1980aagaucaaag aagguaaucu aaaagccuug ccuguuauuu uaaaagcuga
ugugcaagga 2040ucuuuagaag ccuuaaaggc aaguuuagaa aaacuuagaa
augaugagau uaaagugaau 2100aucauucaua guggaguagg aggaaucacg
caaagugaua uagagcuugc aagugcgagu 2160gaaaacucua uaguacuagg
uuuuaacaua cgcccaacag gggaaguuaa agagcgugcu 2220aaggauaaag
gcguagaaau uaaaacuuau aauguaauuu auaaucuuuu agacgaugua
2280aaagccuuac uuggugguau gaugagcccg auuauuucug aagagcaauu
aggacaagca 2340gagauuagac aagugaucaa ugugccaaaa aucggacaaa
ucgcagguug uaugguaacu 2400gaagggguga uuaaucgugg agccaaaauu
cgccuuaucc gugauggagu ugugguuuau 2460gaaggaaaug uaaguucgcu
uaaacgcuuu aaagaugaug cuaaagaagu ggcaaaaggc 2520uaugagugug
gcguagguau agaagggugc gaugauauga gaguggguga uuauauagaa
2580agcuauaaag aaguagagga acaagcaagu cuauga
2616822841RNACampylobacter jejuni 82augcuagcac cuggcauggg
agaauggguu uacaaggcua auuuauuuuu auuuggagaa 60uuugcguauu auuauccuuu
uuucuuauuu auuuuaaauu auguuuauua uaaaagaaau 120uacaaauuag
cuaauuuuac aagaagagag cuuuuuggua uaggauuugc cuuuuucucg
180aguuugcuuu uauuugcagu uuuuuauccu aauucaggcu auauacuaga
gcuugcuuau 240gcgauuuuuu cuacuauuuu ggggcauacu gggagcggaa
uuuucgcucu uuuacuucua 300cuauuuucuu ugguuuuauu guuuccaaaa
uuugcaaaag aaauuuuaaa aauagaauua 360gauuuuacuu auuuauuaaa
aguagagcaa gcuuuuaaau cuuuacuuau gcguguauuu 420gguggagaaa
acgagaaaga agauguaggu aaaucagaac cuauaguucc aaaauuaaau
480auuuugcaag auaguauuua uggaaauuua caaauaaaca aaaaagggga
aaccaauaac 540uuagaacaga uaauuaaaga uagcaauauc aaugcaagua
aaaauucaau uaccacggcc 600aaggaaaauu uugaaaaacu aaaaaaucaa
auuuuagaug aaaccauaga gauugauaaa 660caaaguuuaa aagaaucaag
aaguuuuguu caugagcauu cucaacaagu gcgaaauuuc 720gcacaaaagg
cuaguaaaau gagcauuagu cuugaugaag auuuuaauuu uauuucagaa
780gaagagguag auaugauucc ugaacguuuu uuaaaaccua aaaaacuuga
agauauaaag 840caaauagaua caaauaaaaa uuuagaugag ccaaguuaua
aacguaaaaa uauugaaauu 900ccaguuucua aucaagaagu uaaaccaaaa
auuuuuacaa aagagcuuga acuuagagag 960aauuugauaa aaaaagaaaa
acuagaacaa gaauacaaag ccuaucaaaa ugaaauuuua 1020gaaaauaaag
uaaaacaaga aauuaaaaaa uuagaagaau augaugcgau aaauucaagc
1080gauauuauag aagggaauaa auauaguuuu aauagcccaa aaacaauuaa
aacagaaaca 1140gaagaaucag acaaaauaaa ugaaaauaaa aaucuagaua
aagcagacaa uaucuuugaa 1200uuugcuccca uuguagaaga guuaaaucau
ccuuauauag aaccuacucc uauaaaaaau 1260auaaaugaaa uaguuauaga
agaaaaaaau acacucgauu uuauccaaaa uacagaaacu 1320aaaaucgaua
augaaaaaac aaaugaucaa gaaauuaaac uucaaaaagc aguuuuagcc
1380aaagaaauug cuauuaacca agcuuuauug cgugaaauag agcagggcga
aauagaaaaa 1440ccaaaagauu ucaccuugcc gccauuagau uuuuuagcua
auccaaaaga acacaaacaa 1500gaaaucaaug aaagugaaau agauaaaaaa
auuuauaauc uucuugaaaa auugcgucgu 1560uuuaaaauag gcggcgaugu
uauaagcacu uauguugguc cugugguaac uacuuuugaa 1620uuucguccua
gugcagaugu gaaaguaagu cguauuuuaa auuuacaaga ugauuuaacu
1680auggcuuuaa uggcaaaauc aauccguauu caagcuccaa uaccaggaaa
agauguugua 1740gguauagaag uuccaaauga ugaaauucaa accauuuauu
uaagagaaau uuuacaaagu 1800gaaguuuuua aaaacgcuaa aaguccuuua
accauagcuu uagguaaaga uauaguaggc 1860aaugcuuuug uaaccgaucu
uaaaaaacuu ccgcauuuac ucaucgcagg aacgacaggu 1920agugguaaaa
guguggggau aaauucuaug cuuuuaaguc uuuuauaucg caacucucca
1980aaaaccuugc guuugaugau gauagauccu aagaugcuug aauuuagcau
uuauaaugac 2040auuccucauc uuuuaacucc uguuaucaca gauccaaaaa
aagcagucaa ugcgcuuuca 2100aauaugguag cugaaaugga aagacgcuau
cgcuuaaugg cugaugcaaa aaccaaaaau 2160auagaaaauu acaaugaaaa
aaugaaagaa uuaggcggag aaaaacuucc uuuuauugua 2220guaauuaucg
augaacuugc ugauuugaug augacugcgg guaaagaugu agaauuuuau
2280auaggaagac uugcgcaaau ggcaagagca aguggaaucc acuugauugu
agcuacacaa 2340cgaccuucug uugauguugu aacaggacua auuaaagcga
auuuaccaag uagaauuucu 2400uauaaaguag ggcaaaaaau ugacucuaaa
guuauuuuag augcuauggg ugcugaaagu 2460uuacuuggaa gaggggauug
uuuauuuacu ccuccuggaa caagcucuau agugcguuug 2520caugcgccuu
uugcaagcga auuugaaaua gaaaaaaucg uggauuuucu aaaggaucaa
2580caaagcguag aauaugauga aagcuuuuua aaggaucagc aaagcguagg
uguuacaaca 2640aaugaaagcu uugaugggga agcagaugag cuuuaugaag
aagcuaaaag aguaaucuua 2700gaagauggaa aaacaagcau uucuuauuua
caacgccguu uaaaaaucgg cuauaaccgc 2760ucugcaaaua uuauugaaca
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aaaucuugua a 2841833126RNACampylobacter jejuni 83augaaaaaau
uuuuuuguuu aacuuuaguu uguaaacuuu uugcuuuaag cgaauuugaa 60cuucaucaua
uugauaaagu acauaagcua ggguauagcg gagauacuau uauaauaggu
120guagccgaug augcuuuuaa ucaagaucau auuaguuuaa aggauaagau
uuuaaagucu 180acuuauccua cugauacagc ugggaaacag cuuauaccug
auuugaaaaa aucaacacac 240ggaagucaug uagcagguau agcuguugga
gcaaagauag gcgauagcaa accuuaugga 300guggcuuaug gggcaaaauu
uuauggagcu gggguguuuc caaauggcuc uuacacucaa 360auuccugaua
uuuauaauuu uuucaaagau gugaguauua uuaauaauag cugggguauu
420aauuuuuauc cuuauuuuaa ucuuaaggcu ucaaauucug gauuaguaga
uuguacucaa 480acuaaucaag ggacaagcua uaauaucugu aauacuccuu
uggaauaugu uaugaaagca 540gauaagguug cuaaugauau gaugaggcuu
agcaaggaca agggaguauu aaauguauuu 600gcugcuggua augaaggaau
ucuuagcccu gcuuugcaug cgauuuuacc aaguuaugau 660gaaucuuuaa
gagcuugguu ggcuguugga gcuuuggaug caaaugagau uacuuuagaa
720ucagauggga cuuuaauaau uaaaagucaa gguuuggcug auuuuaguaa
ugguuuuaag 780ggagcuacga auuuuucuuu aguugcugcu ggaguaaaua
uuaauaaugu ugauucaagc 840acuaaugaua aguuuacaaa aaaaagugga
acuucuaugg cagcaccuau gguaagugga 900acagcggcac uggucaagca
aaauuucccc uuuuuggaug guaagcaaau ugcugauaua 960cuauuaagua
cagcaaauaa aaauuauaag gcuccaaaau uuacuguuaa acaaguaacc
1020gauggaacaa aucaaccuaa auuucuuauu guguauauuu cgcaagaucc
accugggaua 1080gaagaugaaa uaaaacggga uuuaaaacag cuuuacaaug
gaauacaagu ucaaguuaau 1140ggacaaugga uugauuauag ugauuauauu
ugggauaaua gagauagugc gcagucacaa 1200aaacuuaaua cuuccacuau
uaguucuauu aauggaguag uuagaguuga gaaagaagaa 1260uuauuuggac
aaggaauuuu agaugcacaa aaggcguuaa aaggacugag uauuuuagau
1320gcaaacagac ucagugauca ggauguauua aaauaugagc aagaaccuaa
uacagcuuau 1380uauacuauaa acacugcagg uuaugaugcu gaauuuucua
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auuaauaaac cuacacauuu agcuaaucuu 1500aacauagggu uaucuaaaga
aggugaaggu auuuugauua ucagugguca aaauacuuau 1560gagggcgcaa
cuuuaaucaa acaaggagaa uuaaagcuua aaggaaaagu uaaaaauaau
1620gcuuauguag aacaaaaagc aauauuaagc gguaauggua uuguagggca
aaauuuaaac 1680aauaaaggca uaguuagacc uggaaaugaa gauuugaaug
auuuaaccgu gcaaggaacu 1740uauacucaag aaggaguuga uucuaaauug
caacuugauu uugguaauua uaaaaauucu 1800aaacugauug caaaaacuua
ugauauuaag agcgggaauu uagaauauau uccuuuaccu 1860aaauacuaua
ucuuaaauaa gccagugaaa auuaauuugg gagauuugga aaaaaguuua
1920ucaucuuuua aucauguuuu gauacaaaau accuaugcuu uaaauuuuga
uuuuguuuua 1980agugaugauu uagugaguau uaauaaaacc uuaauaaaac
cuaauuuaaa gccaaaugcu 2040uacgaaauuc cuaauacaag cuugggaaau
gcuuuaagac aauugcgauc uagggcagac 2100uugagucaga cuuaucagga
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gaauagaagg uucaggguau uuaagcacuu uuaguaauca uaaucaaucu
2220aauuuaaugc aaaauaauau guuauuuacc cuucauccuc uuaauauuaa
uaauuuugca 2280caaaacaaua auaucuuacu ugcuaguacu uauuuaccua
gaauuuuuag caaugaagaa 2340uauuuuuggc aucuuacucc aaguuauaaa
uacuauaaag auaaagauuu uucaggucaa 2400aaaacaggug cuaauaucuc
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2520auggauuuau ugcuuaauua uaaccaugau uuagauuuua uaaaaauauu
aaguggauua 2580gguauaggug uaggauuuaa uacucuuaau cguuuuguag
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cuugguguaa cuaaagauau uauuuuaggu 2700caagauuuua uuuuuaaucc
uuuaauguau uuuacacaua guuuuuuuua ucaagaagau 2760uuuaaagaaa
auaaaagucc uuuugcuaaa aauuaugaaa guuuaaaaca ucauagcaua
2820aaugcaaauu uagguuuuaa ucuugcuaaa aauauagagc aagaugauua
ucaagcuucu 2880uuuucuacuu uuguaauuuu ugaaaaaaga auuuauggaa
gaacuuuaga aaauaaggcu 2940aguuuuguug auuuuccuau ugcuuuuauu
caaaaauaua aauuaaaaga uaauauuuua 3000agucaagguu uuaauucaga
auuuuuauau aaaaacaaug uauuuuggca guuuauguua 3060augaauagau
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3126843270RNACampylobacter jejuni 84augccaaaac gaacagauau
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uuauucugga acucaagccg caaagacuuu aaaagaauua 120ggauaucgug
uaguauuaau caacucaaau ccugcaacca ucaugacaga ucccgaauuu
180gcagaugcga cuuauauaga acccauaaca aaagaaagua uuuuaaguau
uauuaaaaaa 240gaaaaaauug augcaauuuu gccaacuaug gguggacaag
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300gaaguuuaug aaagcggacu uuuaggagau gugaaauuuu uaggcgcaaa
uccugaggcg 360auuaaaaaag gcgaagaucg ucagguuuuu aaagaaugua
ugaaaaaaau uggcauggau 420uugccaaaau cgauguaugc guauaauuau
gacgaagcuu uaaaagccgu agaugaaauc 480gacuuuccuu ugaugauccg
ugcuucuuau acuuuagggg gugcuggaag uggugugguu 540uacaauaugg
acgaauuuaa agaacuuacc aauacugcuu uagcuuuauc accuauucau
600gaaauuuuga uugaagaaag uuuguuaggu uggaaagaau augaaaugga
aguuauacgc 660gauagagcgg auaauuguau cauaguuugu agcauagaaa
auaucgaucc uaugggaguu 720cauacaggag auaguauuac aauagcucca
gcauuaacuu ugacagauaa agaauaucaa 780guuaugcgua augcuucuuu
ugcuauuuug cgugaaauug guguagauac aggcggaagu 840aaugugcaau
uugcuaucaa cccaaaaaau ggaagaauga uaguuauaga aaugaaucca
900agaguuucaa gaucaagugc uuuagcuucu aaggcaacgg guuauccuau
agcaaagguu 960gcgacacuuu uggcaguagg uuuuagcuua gaugagauua
aaaaugauau uacaggaacu 1020ccugcaucuu ucgagccugu gauugauuau
auuguaacaa aaauuccucg cuuuaccuuu 1080gaaaaauuuc caggagcaaa
uacaacuuua gguacagcua ugaaaagugu gggugaggua 1140auggcuauag
gacgcacuuu uaaagaaagu auacaaaaag cacuuuguuc gcuugagcgu
1200ucuuuaagug guuuugauag gguaaaauuu gaagauagaa augaucuugu
uuuuaaaauu 1260cgcaaugcca augaaaagcg uuuacuuuau guugcucaag
cuuuuaggga agguuuuagc 1320guagaagaac uuuaugagcu uuguaaaaua
gauccuuggu uuuuaacaca gauuaaagaa 1380auuguagauu uugaagaaca
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cuaugggcuu uucagauaaa augauagccu ugcuuguaaa uuugaaagau
1500aauuuagaau uaagccaaaa ugauauuuau uauguaagaa ugaagcaaaa
aaucaucgca 1560gaauuuagug aaguggauac uugugcgggu gaauuugaag
ccuuaacucc uuaucuuuau 1620ucaaguauca auguaagcga acucacucaa
aguaaaaacg augcuaagga uaaaaaagaa 1680aaaaaaguga ugauuauagg
uggggggcca aaccguauag gacaagguau agaauuugac 1740uaugcuugcg
uacaugcuuc uuuugcgcuu aaagauaugg guauuaaaac uauuauguau
1800aauuguaauc cugaaaccgu uucgacugac uaugauacaa gugauauuuu
guauuucgag 1860ccuauugauu ucgaacauuu aagagcggug auugagcgug
aaaaaccuga uggagugauu 1920gugcauuuug guggacaaac uccuuugaaa
uuugcuaagc guuuaagugc uuuuggagcu 1980aagauuauag guacuagcgc
aagaguaauu gauauggcag aagauagaaa gaaauuugcc 2040gaauuuauua
caaagcuagg uaucaaucag ccaaaaaauu cuacugcaac aagcguagaa
2100gaagcgguuc uuaaggcuag ugauauaggg uauccugugc uuguaagacc
aaguuauguu 2160uuaggugggc gugcgaugcg cgugguaaau gaugaggcug
aacuuagacu cuauaugcaa 2220gaagcugugg auguaagcga uaaaagcccu
guuuugaucg aucaguuuuu agacaaugcu 2280acagaaauug auguugaugc
gauuugugau ggcaaagaug uuuauguugc aggaauuaug 2340gagcacauag
aagaagcagg aauucauucg ggugacagug cuuguucuuu gccgccuugc
2400aauaucgaug aaaaaaugca agaauuuauu gcacaaaaaa ccgcagauau
ugcuuuaaau 2460uugggaguug uaggacuuuu aaauauacaa uuugcuuuac
auaauaauga gcuuuauaug 2520auagagguaa auccuagagc uagucguacc
auaccuuuug uuaguaaagc uacggguauu 2580ccuuuagcaa aaguggcaac
gcgugugaug uggcaaggaa auuuaaaaga agcuuuaaaa 2640uuuuaugaua
cuuuuaaagu gguuaauuuu gauacuaaaa uuuuacgccc uaaaacucca
2700aaauauauga gcgugaaaga agcaguauuu ccauuugcaa aacuuagugg
aagugauuua 2760gaauuagguc cugaaaugcg uucaacgggu gaaguuaugg
guauaagcaa ggauuuugca 2820aauucuuaug cgaaaaguca aauugcaucg
uuuaaucauc uuccagagca aggcguggua 2880uuuaucuccu uaaaagauaa
ggauaaaaaa uauaccaaaa aaaucgcugc agaauaugua 2940aagcuuggcu
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3000uuugagugcg aacuuguaca uaaaauuuca gaaggacgcc ccaauguuga
agauaaauug 3060aaaaauggag aaauucacuu aguuaucaau acaagcgaua
gucacaguuu uaaaggcgau 3120acgaaaaaaa uucgugaaaa uauuauucgu
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aagaguggcu uaaaucuuga 3270853365RNACampylobacter jejuni
85augaaaaaua ucacauuaac uaaaauaccu auaggagagg gcaaagaacc cuguuuaaau
60ucuaaaaaga uaguuuuauc uuuagcuacu auuucuuuuu uggcaaguug ugcuaaugcu
120aaauuaaauu cugaaauaaa aacuuaugau gaaguuaaua aaaauguuaa
aacuagauca 180gcaaguguuu auagcccuca agcuaagauc aauacgacua
uaaauucuuu acacaaucaa 240caaguaacua uuacuggaaa uggaacauca
aauucuuuaa caaucggauc aaguggaacu 300cuagguagca uaggcaauac
agguaaaauc aucuaugccc augcuaaugg uagcaacacu 360cuuacucuug
caaaucuuac uaauaauaga acuauuaaug guaagauugg uauagaaaac
420aaugguaauu uuacaggaac uauugcuguc aacaccuuug aaaauacagg
ucaaauuaau 480ggacaaauuu acaugggaau uugggguaac aacucaggaa
cucuuaauau agauaaauuu 540gacaauagug gaaccauaau ugacaacaau
aaaggaguuu uuuugaagga aagaauacca 600acauacaaac cuuuaauaac
agugguuuua uuagugcaaa uaagggugua gauauaggca 660acauaggaac
cauaaaaaau uuuaauaaca acggaacuau acaaggcuca gaagugggag
720uggcuauaaa uacaaaaaua gauacuuuua cuaauaaugg uuuuauuaau
uccccuggua 780guggacaaug gaauaauggu auauggauaa guagcaaugc
uaccauagaa aagcuuguua 840acaauggcac aauaaaagga ggacauucug
cuauaauggu aacaucucag cauauaaaaa 900cuguugaaaa uacaggaauu
auacaugcug aaggagaaug ggguucuagu auauuauuag 960aauauggugg
uuuuauagag cauauaauca auacuggaac cauaaguaau aauaauguug
1020guauagguuc agcuuaugga guauuuggaa cacuuaccau aaaagaugga
gguaugguuu 1080auggaaaaua cucggcaaua ggaguggguc gaucgcaaac
ucuaggagau uuauauauag 1140auggacguuc aaauaauggc acaguaagug
gaauuuauag ugaagaacau ggaauuuuau 1200uagagaauaa cucacgaacu
caaaaaauag aacuuaaaaa uggcggcauu auaaaaggua 1260auaucgaugg
uauaagauug auaaacucgg ccucuuuaag uggagaaaug auuuuaucug
1320gcgaagguuc uaggguagaa gguggaagag guguugguau auugaaucga
aguggaaaaa 1380uagaaggcuc uauaaaagua gaagauggag caacuguuac
ggcuacuucg aaucgagcca 1440uagcuaacuc uggcucagga aguauaacag
gugguauuac uguuaguggg aaaaacacua 1500aacuugaagg aaauauaauc
aauacaggua augcuucuau agguagugau aucaagauag 1560aagguggagc
uaagguagaa gguggucuug uuaaucaagg uaauggaagu aucucaggaa
1620guguucaagu aagugguggu aguaguauug auuccauuac caaugagggu
aauggagcua 1680uuucugguuc aauuacugua uauaaagaua guaaacuuga
uucuaucacu aacacuucua 1740caucaucuac agguauaagu gguucuauua
cuaacaauag ugauaauaaa cuugaaauuu 1800cuaauucagg uaauaucggu
gguaagauug aaaguacagg uagugcugau auggugauua 1860gcaauaguaa
ugguggaacu auuaguggcg guauuaguuc uucaggaagu ggaagcacua
1920guauuuccaa uucacaaggc ucaaccauaa auaacggcau cacuguuuca
ggaucagcac 1980aaguugaaau cuccaaucaa ggcucaguag guaaagauga
aaaugguaau acaguaacua 2040acaaugguag ugguaguguu ggaaucaaag
auuggcuugu uucuacagau aaaaacacag 2100guaaauuaaa cacugugguu
auagguggaa guagagcuuu uaauguaaaa guagaaaaca 2160ucaccguaga
ucaaagcaau guugaucuug aagagcuaaa cgauauaaac aacaucaucu
2220cagguguuaa ucaaaacaau auuggcaaua uaggaaccaa uggaagugga
gaaauaucuu 2280uaagcuuuga uccaauaaca ggaaagcuua cuacagauuu
uaaucuuaau gcuuccauau 2340caggugcaac cuuuagaucu uuaaucucua
cuacuucaag aagaucaacc uuuauagaua 2400auguuauggg uaauucuaug
caaagcuuug cuuuagcuuc uucaaguaaa ucucaaagca 2460ucgcuauguc
ugaaaagggu aauuuauaug cagaugcaag ugauuauaua aagagugauu
2520uaaauaaugg aagcuauggu ucuaauaaag aacauucuuu auucauccuu
ccuuauacuu 2580cuucacagaa uguugaacuu ucuuuaaaug aagaaaguaa
aggacacacu aaaggaacca 2640uuauagguua uucuacuuua aaagacagug
gaauauaugg uguguaugca gguuaugaag 2700auacaaaaau ggguucaacu
uauuuugaua uuaauaauag aaccuauuau gcagguuuaa 2760aauacuuuaa
uaccuuauuu acuacagaaa aaggucaaga aguuuauauc aaagcucaag
2820guaaagcugc uuugauuaaa aaugauuuaa cugaaaaaau aggaaauaau
gaagcuaaag 2880cugaaccuaa uucuuaugcu uauggaguca auacagccuu
agguaugaau uuuauuucua 2940auaaagauau auucucuccu gaaauagguc
uugcuuauga aggagguuau acugaagcuu 3000uuucuaugaa agacaccaua
ggacaagcaa caguuaaagg uggagaaaga accuaugcaa 3060acuauuuaaa
ucuuuucucu acuaaaacaa gucuuacuug guuuagagau ugguugccua
3120auuuaaaaac uucaguagaa cuuggagcua aguuuaauau caauccuaaa
guagaagcug 3180aggcuagauu ugguaauaua aaaguaagug augaguuuga
uuuaccaaga guacaaaaau 3240uuguaagcac uucuuuuauc guuccuguua
augaagcuuu cuauuuuagu uuaaacuaua 3300auggaauguu ugauaaagau
ggcaauaccc auacaggauu ugcucaguuu aauuaucuuu 3360gguaa
3365863436RNACampylobacter jejuni 86augaauaaaa cugcauuaac
uaaaacauac acaaaagaua uacaaaauuc cuguuuaaau 60ucuaaaaaga uaguuuuauc
uuuagcuacu auuucuuuuu uggcaaguug uacucaugcu 120acacuuacuc
cugaaauaaa aacuuacgaa gagacaaaua gacaugcuaa agcuagguca
180ggucuucaau cuagaaauuc gaauaaugag acuauaaaua auuuacaaac
uuuaacuaaa 240acuauaagug acaccggcaa uacucuaguu auagaaagua
guggaacuau uacuauuucu 300aaugaugggc aacaagcagu aaacuuucaa
ccaaauucuu caacuucuac cuuuuuaaau 360aaaggaacuc uuauaggugg
aaauaauacu gcuaguguuc aacuaggagc agcaaaugga 420aauaacggug
uuagcauaga aaccuuuaau aaucaaggua ucauagguaa ugguucuucu
480aaauuuggag uaacaguuuu uggggggggg gaguaaagau aacccuaaau
cuauaaucaa 540uaauuuuagu aauaguggaa cuauucacuc uaauacuggu
gaaaguauuu auuuugguaa 600cgccaaaaua ucaaguuuug uuaauagugg
gacuauuaag aguaaacaag gugcaggagu 660aaauauaucu caaggaacaa
guauagagaa uuuuaacaau accggaaccg gaauuauuga 720aggcaagaga
auggguguaa auguucguuc aacaauaaau accuucguca augacgguuu
780aauugcugca acgaacgaug gaauacagau aaaugcuaau guaaaaacau
uaauaaauaa 840gggaaccaua aaaggagaug cuauaucuau aagaucauua
gguggaacua uagaaacauu 900gaccaaugaa ggcauuaugu augguaaaag
ugcugguauu uacaugaaca gaagucuugu 960uaaaacucuu acaaauagcg
guaccauuaa ucaaaacaau ucggcaacuu ggucugcugg 1020uauaaaacuc
gaaaauggua guaucauaga aaauaucauc aacacaggau cuauccguuc
1080uaaugcuuuu ggaauaucug uaacuggugg uaaauuugga acacuuacca
uaaaagaugg 1140agguaugguu uauggaaaau acucggcaau aggagugggu
cgaucgcaaa cucuaggaga 1200uuuauauaua gauggacguu caaauaaugg
cacaguaagu ggaauuuaua gugaagaaca 1260uggaauuuua uuagagaaua
acucacgaac ucaaaaaaua gaacuuaaaa auggcggcau 1320uauaaaaggu
aauaucgaug guauaagauu gauaaacucg gccucuuuaa guggagaaau
1380gauuuuaucu ggcgaagguu cuaggguaga agguggaaga gguguuggua
uauugaaucg 1440aaguggaaaa auagaaggcu cuauaaaagu agaagaugga
gcaacuguua cggcuacuuc 1500gaaucgagcc auagcuaacu cuggcucagg
aaguauaaca ggugguauua cuguuagugg 1560gaaaaacacu aaacuugaag
gaaauauaau caauacaggu aaugcuucua uagguaguga 1620uaucaagaua
gaagguggag cuaagguaga agguggucuu guuaaucaag guaauggaag
1680uaucucagga aguguucaag uaaguggugg uaguaguauu gauuccauua
ccaaugaggg 1740uaauggagcu auuucugguu caauuacugu auauaaagau
aguaaacuug auucuaucac 1800uaacacuucu acaucaucua cagguauaag
ugguucuauu acuaacaaua gugauaauaa 1860acuugaaauu ucuaauucag
guaauaucgg ugguaagauu gaaaguacag guagugcuga 1920uauggugauu
agcaauagua augguggaac uauuaguggc gguauuaguu cuucaggaag
1980uggaagcacu aguauuucca auucacaagg cucaaccaua aauaacggca
ucacuguuuc 2040aggaucagca caaguugaaa ucuccaauca aggcucagua
gguaaagaug aaaaugguaa 2100uacaguaacu aacaauggua gugguagugu
uggaaucaaa gauuggcuug uuucuacaga 2160uaaaaacaca gguaaauuaa
acacuguggu uauaggugga aguagagcuu uuaauguaaa 2220aguagaaaac
aucaccguag aucaaagcaa uguugaucuu gaagagcuaa acgauauaaa
2280caacaucauc ucagguguua aucaaaacaa uauuggcaau auaggaacca
auggaagugg 2340agaaauaucu uuaagcuuug auccaauaac aggaaagcuu
acuacagauu uuaaucuuaa 2400ugcuuccaua ucaggugcaa ccuuuagauc
uuuaaucucu acuacuucaa gaagaucaac 2460cuuuauagau aauguuaugg
guaauucuau gcaaagcuuu gcuuuagcuu cuucaaguaa 2520aucucaaagc
aucgcuaugu cugaaaaggg uaauuuauau gcagaugcaa gugauuauau
2580aaagagugau uuaaauaaug gaagcuaugg uucuaauaaa gaacauucuu
uauucauccu 2640uccuuauacu ucuucacaga auguugaacu uucuuuaaau
gaagaaagua aaggacacac 2700uaaaggaacc auuauagguu auucuacuuu
aaaagacagu ggaauauaug guguguaugc 2760agguuaugaa gauacaaaaa
uggguucaac uuauuuugau auuaauaaua gaaccuauua 2820ugcagguuua
aaauacuuua auaccuuauu uacuacagaa aaaggucaag aaguuuauau
2880caaagcucaa gguaaagcug cuuugauuaa aaaugauuua acugaaaaaa
uaggaaauaa 2940ugaagcuaaa gcugaaccua auucuuaugc uuauggaguc
aauacagccu uagguaugaa 3000uuuuauuucu aauaaagaua uauucucucc
ugaaauaggu cuugcuuaug aaggagguua 3060uacugaagcu uuuucuauga
aagacaccau aggacaagca acaguuaaag guggagaaag 3120aaccuaugca
aacuauuuaa aucuuuucuc uacuaaaaca agucuuacuu gguuuagaga
3180uugguugccu aauuuaaaaa cuucaguaga acuuggagcu aaguuuaaua
ucaauccuaa 3240aguagaagcu gaggcuagau uugguaauau aaaaguaagu
gaugaguuug auuuaccaag 3300aguacaaaaa uuuguaagca cuucuuuuau
cguuccuguu aaugaagcuu ucuauuuuag 3360uuuaaacuau aauggaaugu
uugauaaaga uggcaauacc cauacaggau uugcucaguu 3420uaauuaucuu ugguaa
3436873561RNACampylobacter jejuni 87auggguaaaa uuaugaaaac
uauggacgga aaugaggcug cugcauacgc ugcguaugcu 60uuuacugaag uugcuggaau
uuauccuauu acaccuaguu cuccuauggc ugauuauacc 120gauauguggg
cagcugcagg aaaaaaaaau cuuuuuggag uuccuguaaa aaucguagaa
180augcaaagug aagcaggagc ugcagguagu gugcauggau cuuuacaagc
uggggcuuug 240acuacaacuu auacggcuuc ucaaggauua cuccuuaaaa
uuccaaauau guauaaaaua 300gcaggucaau uacuccccug uguaauccau
guagcagcgc guucuuuggc ugcucaagcu 360uugucuaucu uuggugauca
ucaagauauu uaugcagcaa gacaaauugg uuuugcuaug 420cuuuguucgc
auucugugca agaaaccaug gacuuagcug guguagcaca uuuagcugca
480auuaagggaa gagugccauu uuuacauuuu uuugaugguu uuagaacaag
ccaugaaauu 540caaaaaguug aaguaaugga uuaugcgcau uuugauagau
uauuagauag agaagcuuug 600cuugaauuua gaaauaaugc uuuaaaucca
gaaaauccaa aaacacgugg aacagcacaa 660aaugaugaua uuuauuuuca
aacuagagaa guaagcaauc guuuuuacga ugcuuuaccu 720gauguuguua
augaauauau gcaagaaauu ucaaaaauua cagguagaga auacaaacca
780uuuacuuauu auggucacaa agagccagag ugugugauug uugcuauggg
uucuguaacu 840caagcacuug aagaaguggu ggacuaucuu aaugccaagg
gugaaaaggu agggauuuua 900aaaguuuauc uuuaucgccc auuuaguuua
aaauauuucu uugauguuau gccaaaauca 960gugaaaaaaa ucgcgguuuu
agauagaacc aaagaaccag gaagucucgg agaaccacuu 1020uaucuugaug
uaaaaucagc uuucuaugga agagaaaaug cuccuguuau agugggugga
1080agauacggac uuucuucaaa ggauguugau ccugcucaaa ugauagcagu
auuugaaaau 1140cuuaaacuug auaauccaaa agauggcuuu acuguaggua
uaauugauga uguaacucau 1200acuucacuga guacuggaga gaaaauuuca
cuuggagaug aaaguacgau cgaauguuua 1260uuuuacggac uuggugcuga
uggaacugug ggugcaaaua aaaacucgau uaaaaucauc 1320ggagauaaaa
cggauuuuua cgcucaagcu uauuuugcuu augauucuaa aaaaucaggg
1380gguuauacaa gaagccauuu aagauuuucu aaaaaaccua uccguucaac
uuaucuuguu 1440ucaacuccgc auuuuaucgc uuguucggua gcggcuuauu
uggaaauuua ugauguuuua 1500gcaggaauuc gcaaaggagg aacuuuccuu
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aguaaagaaa acuuuagcug aaaaagaagu aaauuuuuau 1620aucaucaaug
caaccaaacu agcucgugau auagguuugg gaaaucguac aaauaccauu
1680augcaaucag ccuuuuucaa acuugcaaaa aucauuccuu augaagacgc
acaaaaauac 1740augaaagagc uugcguauaa aucuuauagu aaaaaaggcg
augcuauugu agaaaugaau 1800uacaaggcua uugauguggg ugcugaugga
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aaaagaacaa accaaugccu auaaagguac ugaauuuguu 1920gaaaaaaucg
uaaaaccuau gaaugcggcu aagggugaug auuuaccugu uucagccuuu
1980uuagguuaug aagauggaag uuuugaacac ggcacaaccg aauacgaaaa
acgcgguguu 2040gggguuaugg uaccaagaug gauagaggca aauuguauuc
aaugcaauca augugcuuca 2100guuuguccgc augcuguuau caggccauuu
uugauuaaug augaagaaau ggcaaaugca 2160ccucgcggug uaaaagauca
ugcuuuagaa gcuaaaggaa ccaaaggaga aaaauuaagc 2220uuuaaaauuc
aaguuucucc acuugauugu acaggcugug agcuuugugu ucaugagugu
2280ccuacuaaag aaaaaucuuu gguuauggua ccacuucaag aagagaugga
uuuuggugag 2340caagaaaaug cggauuauuu auuuaaagaa auuacuuaua
aagaugauau uuuaaauaaa 2400gaaaccacaa aaggagcgca auuugcccaa
ccuuuauuug aauuccaugg ggcauguccu 2460ggaugugggg aaacuccuua
uauuacuuua auuacaagau uguucgguga aagaaugauu 2520guggcuaaug
cuacagguug uaguucuauu uauggggguu cagcuccauc aacuccuuau
2580agaaaaagug ugaaaaaugg acacgguccu gcuuggggaa auucacuuuu
ugaagacaau 2640gcugaguuug guuuggguau gaaaauugca acugaaaaua
caagacaccg cauugaacau 2700aucaugaaug aaaguaugca agaaguucca
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ugcuaugucu guggaaauua aagauaaaau gaucccuauu 2820uuagagcaaa
auaaaaauau uaaagcugua caagauauau uagagcuuaa acaguauuua
2880aguaaaaaau cucacuggau uuuugguggu gaugguuggg cuuaugauau
aggcuauggc 2940ggacuugauc auguuuuagc aaguggagaa aauguaaaua
uuuuagugcu ugauacagaa 3000guuuauucua auacaggcgg ucaaaguuca
aaaucuucua gaacaggagc uguagcacag 3060uuugcagcag caggcaaacc
uauacagaaa aaagaucuag gucaaauugc uaugacuuau 3120gguuauauuu
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3180aucacugcag cugaggccua ugauggacca ucuuugguga uuuguuauuc
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aaggugagcu ugcuacaaaa 3300ugugguuauu ggccacuuua uaccuuugau
ccucguuuag aagagcaagg aaaaaauccu 3360uuaacucuaa caggaaaaga
accugauugg gauuuauaug agcaauuuuu gaugaaugaa 3420gugcguuaua
auucacuuaa aaaagcaaau ccugaacacg cugcugagcu uuuugaacgc
3480aauaaaaaag acgcucaacg ucgcuauaga cagcuuaagc guauugcuau
ggcugauuau 3540agcaaugagg ucgaaagcug a 3561884137RNACampylobacter
jejuni 88augugcgaua uguuagauaa uaaauuagga aaucguuuaa gaguagacuu
cucaaacauu 60ucaaaacaaa uagaaauccc aaaucuucua caauuacaaa aaaagaguuu
ugauuauuuu 120uuaaaucuug auaaugguga aagugguaua gaaaaaguuu
uuaaaucaau uuuuccuauu 180caugauccgc aaaauagauu gagucuugaa
uauguuagua gugaaauagg caagccaaaa 240uauaccauuc gcgaaugcau
ggaaagaggu uugacuuauu cuguaaauuu aaaaaugaaa 300auccgucuua
cucugcauga aaaagaugaa aaaacagggg aaaaaguugg cguaaaagau
360auuaaagaac aagaaauuua uauccgcgaa auuccuuuaa ugacagaucg
uguaucuuuu 420aucauuaacg ggguugagag agugguugua aaucaauuac
auagaagccc ugguguuauu 480uuuaaagaag aagaaaguuc aacgguugca
aauaagcuug uuuauacagc acaaauuauu 540ccugaucgug guucguggcu
uuauuuugaa uacgaugcca aagauguuuu guauguaaga 600aucaauaagc
guagaaaagu uccuguaacu augcuuuuua gagcuuuagg guauaaaaaa
660caagauauua uuaaguuguu uuauccuauu cagacuauac augugaaaaa
agauaaauuu 720uuaacagaau uuaauccaaa ugauuuuaug gauagaauag
aauaugauau uaaagaugaa 780aaaggaaaaa ucguucauca agcuggaaaa
agacuuacaa agaaaaaagc agaacagcuu 840auuaaagacg guuuaaaaug
gauagaauau ccuguagaaa uuuuacuuaa ucguuauuua 900gcaaauccua
uuauugauaa agaaagugga gagguuuuau uugauucuuu aacuuuguua
960gaugagagua agcuugcuaa gauuaaagag caaaaaaguu uugauauagc
aaaugacuug 1020gcuaauggcg uugaugcagc gauuauuaac uccuuugccc
aagauggaga aacucuaaaa 1080cuucuuaaac aaagugaaaa uaucgaugau
gaaaaugauu uagcugcgau uagaauuuac 1140aaaguuaugc gcccagguga
accuguggua aaagaugcag cgaaggcuuu uguaaaugau 1200uuauucuuca
auccugaaag auacgaucuu accaagguag gucguaugaa aaugaaccau
1260aaauuagguc uugaaguacc ugaguauguu acuguuuuga cuaaugaaga
uauuauuaaa 1320acugcaaaau auuuaauuaa aguaaaaaau gguaaaggac
auauugauga uagagaucau 1380uuaggaaauc gucguauccg uucuauaggu
gaacuuuuag ccaaugaacu ucauuuaggu
1440cuugcaaaaa ugcaaaaggc gaucagagau aaauuuacuu cuuuaaaugc
ggaucuugau 1500aaaguaaugc cuuaugauuu aaucaauccu aaaaugauua
cuacaacuau cauugaauuu 1560uuuacaggag gucagcuuuc gcaauuuaug
gaucaaacca acccuuuaag cgagguuacu 1620cauaagcguc guuugucagc
gcuuggugaa gguggacuug uaaaagaaag agcagguuuu 1680gaagugcgug
auguucacgc gacacauuau gguagaauuu guccaguuga aacaccugaa
1740ggucaaaaua uugguuugau uaacacucuu ucuacuuaug caaaaguaaa
ugaacuuggc 1800uuuguugaag cgccauauag aaaaguugua aaugguaaag
ugacuaauga aguuguuuau 1860cuuacagcga cacaagaaga aggcuuguuu
aucgcaccag cuucaacuaa gguugaugcu 1920aaaggcaaua uaguagaaga
auuuguugag gcuaggcaag auggugaaac uauacuugca 1980agacgcgaag
aagugcaauu aaucgaucuu ugcucaggua ugguuguugg aguugcagcu
2040ucuuugauuc cguuuuuaga gcacgaugau gcaaacagag cgcuaauggg
uucaaacaug 2100caacgucaag cugugccacu ucuuacugcu ucagcuccga
uaguaggaac agguauggaa 2160caaauuauug cgcgugaugc uugggaagcu
guuaaagcaa agcguggcgg ugugguugag 2220aaaguggaua auaaaagcau
uuucauuuua ggugaagaug auaaaggucc auuuauugac 2280cauuacacua
uggagaaaaa uuuaagaacc aaucaaaaua caaauuauau ucaacacccu
2340auuguuaaaa aaggugauau aguaaaagca ggacaaauua ucgcagaugg
uccuucuaug 2400gaucaaggcg aacuugcuau agguaaaaau gcuuuaaucg
cuuuuaugcc uuggaauggu 2460uauaacuaug aggaugcuau aguuguaagu
gagagaauua uucgugaaga uacuuuuaca 2520agcgugcaca uuuaugaaaa
agaaauugaa gcaagagaac uaaaagacgg uauagaagaa 2580auuaccaaag
auauuccaaa uguaaaagaa gaagauguug cgcaucuuga ugagagcggu
2640auugcaaaaa uugguaccca uaucaaacca gguaugauuu ugguggguaa
gguuucucca 2700aaaggugagg uuaaaccaac uccugaagaa agacuuuuaa
gagcgauuuu uggagaaaaa 2760gcaggacaug uugugaauaa aucccuuuau
gcaaccgcuu cuuuagaggg uguuguugug 2820gauguuaaaa uuuucacuaa
aaaaggcuau gaaaaagaug aucgcgcaau aaaaucuuau 2880gauaaagaaa
aaauggcuuu agaaaaagag caucaugaua gacuuuugau gauggauaga
2940gaagaaaugc uucguguuug ugcucuucuu uccaaagccu cuuuaaauag
cgaucaaaaa 3000auuggagaua aaaauuauaa aaaaggacaa acugcagaua
uuagcgaacu ugaaaaaauc 3060aaucguuuua cuuuaacaac uuugauuaaa
gcuuauucua aagagauuca aaaagaauac 3120gaugauuuaa aaaaucauuu
ccaaaaugag aagaaaaaau uaaaagcaga acacgaugaa 3180aagcuugaaa
uuuuagaaaa agaugauauu uuaccaagug ggguaauuaa gcuuguuaaa
3240guuuauauug cuacaaaaag aaagcuuaaa gucggugaua aaauggcagg
acgucaugga 3300aauaaaggua uaguuucaac uauaguuccu gaaguagaua
ugccuuauuu gccaaauggu 3360aagagcgugg auauugcgcu uaauccacuu
ggcguuccaa gucguaugaa uauaggucaa 3420auuuuagaaa gucauuuagg
uuuaguugga cuuagacuug gggaucaaau ucaagaaauu 3480uuugauagaa
agcaaaaaga uuuucuuaaa gaauuaagag cgaaaauacu ugaaauuugu
3540ucuauuccaa gacuugcaaa cgaaaaagaa uuuauuaaaa gcuuaagcga
ugaagagcuu 3600uuaaacuaug cuagagacug gaguaaagga guuaaauuuu
cuacuccugu uuuugaaggu 3660guaaauauag aagaauuuag caagcuuuuu
aaaauggcua agauagauau ggauggcaaa 3720acagaacuuu augauggacg
cacgggggaa aaaauugcag agcguguuca uguaggaugu 3780auguauaugc
uaaaacucca ucacuugguu gaugaaaaag uucaugcaag aaguacagga
3840ccuuauagcu ugguuaccca acaaccugug ggugguaaag cacucuuugg
gggacaaaga 3900uuuggagaaa uggaaguuug ggcacuugaa gcuuauggag
cggcucacac uuuaagagaa 3960auguuaacca uaaaaucaga ugauguagaa
gguagauuua gugccuauaa agcauugaca 4020aaaggugaaa auguuccagc
aacaggaauu ccugaaacau uuuuuguauu aaccaaugag 4080cuuaaaucuc
uugcuuuaga uguugagauu uuugauaagg augaagauaa ugaguaa
4137894491RNACampylobacter jejuni 89auggauuuag aaaauauccu
agaaaauaau caaagcauag guuuauauca cccaaagaac 60gaacacgaug ccugugguau
cgcugcaguu gcuaauauac gcgguauugc uucuuauaag 120guuauuugug
augcuuuaga aauuuugaug aaucuugaac accgaggugg uacaggagcu
180gaagaaaauu caggugaugg ggcggggauu uuaauccaaa uuccacauga
uuuuuuuaaa 240acucaagaau uggguuuuga acuuccuaaa aagggugauu
augcuguagc acagauguuu 300uuaucaccca auacugaugc aaaagaagaa
gcaaaagaaa uauuuuuaca agguuuaaag 360gauaaaaaac uugaauuuuu
agguuuuaga gaagugccuu uuaauccuag cgauauaggu 420gcaagugcuu
uaaaagcuau gccuuauuuu uuacaagccu uuguaaaaaa accaaguaaa
480auaagugcag gacuugaguu ugaaagagug cuuuauagua cgcgucgacu
uauagaaaaa 540agagcuauua augugccaaa auuuuauuuu ucaaguuuuu
cuucacgcac cauaguuuau 600aaaggaaugc uucuuucaac ucaauugagc
gauuuuuauc uugauuuuaa agaugucaau 660augaaaucag ccaucgcuuu
ggugcauucu cgcuuuucga cuaauaccuu cccaaguugg 720gaaagagccc
auccaaaccg uuauauggug cauaauggag agauuaauac cauacgcgga
780aauguugaua gcauaagagc uagagaaggc uugaugcaaa gugaguauuu
ugaaaauuua 840gaugaaauuu uuccuaucau agcuaagcuu agcagugauu
cugcaauguu ugauaauacu 900uuagaauuuu uagcucuaaa uggucguacu
uuagaagaag cuuuuaugau gauggugcca 960gaaccuuggc auaaaaauga
aaauauggaa agcaaaaagc gugcuuuuua ugaauaucau 1020ucuuuauuga
uggagccuug ggauggaccu gcugcuauag uuuuuacuga uggagugauu
1080augggggcga guuuggauag aaaugguuuu cguccuucaa gguauuaucu
uacaaaagau 1140gauaugcuca uacuuucaag ugaaacaggg gcuuuaaaac
uugaugaaaa aaauaucaaa 1200gcuaaaaaac gcuuagaacc uggaaaacuu
uuacucguug auacagcaag agguaggguu 1260auagcugaua augaaaucaa
agagcauuau gcuaaugcua agccuuauaa aaaauggcuu 1320aaaaauuuag
uugaacuuga aaaacaaaaa agcggaguuu auaaacauca guuuuuaaaa
1380gaagaugagg uuuuaaaacu ucaaaaagcu uuugguugga guuaugauga
gcuuaaaaug 1440agcguggcug cuauggcuca aaaugguaaa gaagcuauag
cagcuauggg uguagauacu 1500ccuuuggcua uacuuucuaa aacuuaucaa
ccuuuguaua auuauuuuaa acaacuuuuu 1560gcacaaguaa cuaauccucc
ucuugaugcc auaagagaag agauuguuac uucuacaagg 1620auuuaucuag
gaagcgaagg caauuuauua aaacccgaug aaaacaaugc aaaacgcgua
1680aaaauagccu ugccugugau aagcaaugaa gaacuuuuug aaguuaaggc
uuuaaauaaa 1740uuucaaguua aagaauuuuc uauacuuuau gauuauucua
aaaaaacuuu agaaaaggcu 1800uuagaugaac uuugcguuaa gauagaagau
gagguuaaaa aagguguuuc uauuaucauu 1860uuaagcgaua aaggagugga
ugaaaaaaac gcuuauaucc cugcuuugcu ugcuguuucu 1920ggagugcaua
aucaucuagu aagaaaaaau uuaagaacac auacaagucu uaucaucgaa
1980aguggugaac cuagagaaau ucaucauuuu gcaugucuuu uagguuaugg
ugcgacuguu 2040auuaauccuu auuugguuua ugagaguaua caaaaacuca
uugccaauaa agauuuaaau 2100uuaaguuaug aaaaggcggu agaaaauuuc
aucaaggcaa guucaagugg uauagucaaa 2160auugcuucua aaaugggggu
uucuaccuug caaaguuaua auggcucugc acuuuuugaa 2220ugucugggcu
uaagcucuaa ggugauugau aaauacuuca cuucuacaac uucacgcauu
2280gaagguaugg auuuagaaga uuuugaaaaa gaacucauug cuuuacacaa
acaugcuuuu 2340aacgauacac auaaggcuuu agauucuaaa ggaauucaug
guuuuagaag ugcuaaagaa 2400gaacauuuaa ucgauccgcu ugugauuuuu
aaucuucagc aagccugucg caacaaagac 2460uauaaaagcu uuaaaaaaua
cucggcuuua guagaugaaa aacaaguuaa uuugcguucu 2520uugauggaau
uugauuuuag ugaagcuauc aguauagaua agguugaaag uguagaaagu
2580auaguuaaac gcuuuagaac aggggcgaug aguuaugguu cuauuucuaa
agaagcacac 2640gaguguuuag cacaggcuau gaauaaaaua ggugcuaaau
caaauucagg ugaagguggu 2700gaggaugaag aacgcuauga aaucaaagag
gguguggaua aaaacucagc cauuaagcaa 2760guagcaagug ggcguuuugg
cguggauuua aacuacuuaa gucaugcaaa agaaauucaa 2820aucaaagucg
cucaaggugc aaaaccaggu gagggcggac aauuaauggg cuuuaaaguc
2880uauccuugga uagcuaaggc uagacauucu acugcaggug ugacgcuuau
uuccccaccu 2940ccucaucaug auauuuauuc uauugaggau uuggcucaac
ucauuuauga uuuaaaacau 3000gcgaacaaag acgcuaaaau uucaguaaaa
cuuguaagcg aaaauggcau aggaacgguu 3060gcugcaggug uggcuaaggc
aggcgcgaau uuaauccuug uuucagguua ugauggaggu 3120acgggugcaa
guccuagaac uucuauaccg caugcuggaa uuccuuggga guuaggcuua
3180gcugaaacgc aucaaacuuu gaucuuaaau aagcuuagag auaggguaag
auuagaaacu 3240gauggaaagc uuaugaaugg gcgugauuua gccauagcag
cacuuuuagg agcugaagaa 3300uuugguuuug caaccgcucc uuugauuguu
uuagguugua caaugaugag aguuugucau 3360cuaaauaccu guccuuuugg
uauagccacu caagauacag aacuuagaga ucguuucaaa 3420ggcaaaguag
augaugugau uaauuucaug uauuuuauag cugaggagcu uagagaauac
3480auggcaaggc uugguuuuga acgccuagau gauaugauag gucgcgugga
uaaacuccgc 3540caaaaaagug ugcaagguaa agcaggaaag cuaaauuuag
auaaaauuuu aaaauccuug 3600ccuacuuaua auagaaccgc ugugcauuuu
aaagauuaua aagacaauaa acuugaaaaa 3660acgauugauu auagaauuuu
acuaccacuu uguaaaaaug cuguggagaa aaaagagccu 3720aucaaacuuu
cuuuagaagu aggaaaucaa agucguacuu uugcaacuau gcuuucaagu
3780gaaauuuuaa aaacuuaugg aaaagaugcu uuagaugaag auaguaucca
uaucaaagcu 3840auaggcaaug caggcaauag cuuuggagcg uuuuuguuaa
aggguauuaa acuugagauu 3900auaggcgaua gcaaugauua uuuaggcaag
gguuuaagug gugguaaaau cauagcaaaa 3960auuucaaaug aagccacuuu
uucaccugaa gaaaauauca ucgcaggaaa ugcuuguuua 4020uacggagcua
caaaagguga aguguauuua gaugguauag caggggaaag auuuugugua
4080agaaauucag gggcuuuggc ugugguuuua ggaacaggcg ugcaugguug
ugaauauaug 4140acagguggac aaguuguagu ucuuggagau gugggugcga
auuucgcugc ugguaugagu 4200gggggcguug uuuauaucuu uggaagacac
aaugaagcuc auguaaauac cgagcuugug 4260gauauuaaag aucuuaaugc
uaaggaugaa aaagaauuaa aagcugugau agaaaaacau 4320aucacuuaua
cagauucuaa aaaggcuaaa gauauuuuag aaaaauucga uaaaaaagac
4380uuuuuuaaag ucaugccaag agauuaugaa aagauguuaa aaaugcuuga
ucuuuguaaa 4440aaugaaaaag auccaaauuu agcagcauuu uugaaaauca
cacaaaaaua a 4491904554RNACampylobacter jejuni 90augaguaaau
uuaaaguaau agaaauuaaa gaagaugcaa gaccuagaga uuuugaagca 60uuucaacuaa
gacuugcaag uccugaaaaa aucaaaucau ggucuuaugg agaaguaaaa
120aaaccagaaa cuauuaauua uagaaccuua aagccugaaa gagaugggcu
uuuuugugca 180aagauuuuug gaccuauaag agauuaugag ugucuuugug
guaaauacaa aaaaaugcgu 240uuuaaaggcg uuaaguguga aaaauguggu
guugaaguag caaauucaaa agugcgucgu 300ucuagaaugg ggcauaucga
gcuuguaacu ccuguggcuc auauuuggua uguuaauucu 360cuuccaagcc
guauaggaac gcuuuugggu guuaagauga aagaucuuga gcgcgugcuu
420uauuaugaag cuuauauugu ugaaaaccca ggugaugcuu ucuaugauaa
ugaaaguacu 480aaaaaagugg aauauugcga uguuuugaac gaagagcaau
aucaaaauuu aaugcaacgc 540uaugaaaaua gcggcuuuaa agcaagaaug
gguggagaag uuguucguga uuuacuugca 600aauuuagauc uuguagcgcu
uuuaaaucag cuuaaagaag aaaugggggc uacaaauuca 660gaggcuaaga
aaaaaacuau cauuaaacgc uuaaaaguug uagaaaauuu cuuaaauuca
720aauuuaaacg ccaauaccga uagcgacgaa gcuguaccaa aucguccuga
auggaugaug 780auuacaaauc uuccaguuuu accgccagau uugcguccuu
uaguggcuuu agaugguggg 840aaauuugcag uuucugaugu gaaugacuua
uaucgucgug uuaucaauag aaauacacgu 900cuuaaaaaac uuauggaacu
ugaugcaccu gaaaucauua ucagaaauga aaaaagaaug 960cuucaagagg
cuguugaugc gcuuuuugac aacggucguc gugcuaaugc uguaaaagga
1020gcaaauaaac gcccauugaa aucuuuaagu gaaaucauca aagggaaaca
aggacguuuu 1080agacaaaauc uacuagguaa aaggguggau uucucagguc
guagcguuau uguuguaggu 1140ccaaaacuua gaauggauca augcgguuua
ccuaaaaaaa uggcuuuaga gcuuuuuaag 1200ccacaucuuu uagcuaagcu
ugaagaaaaa gguuaugcaa ccacaguaaa acaagcuaaa 1260aaaaugauag
aaaauaaaac caaugaaguu ugggaauguu uagaagaggu aguaaaggga
1320cauccuguua ugcuaaaccg ugcaccuacu uugcauaagc uuucuaucca
agcuuuucau 1380ccuguuuuag uggaagguaa ggcuauacaa cuucauccuu
ugguuugugc agcauuuaac 1440gcagacuuug auggggauca aauggcagug
cauguaccgc uuucucaaga ggcuauugca 1500gaauguaaag ugcuuaugcu
uucaucaaug aauauucuuu uaccagcaag cgguaagucu 1560guaaccguuc
caucgcaaga uaugguuuua ggaauuuauu aucuuucguu ggaaaaagca
1620ggugcuaaag guucgcauaa aauuuguaca ggcauugaug aagugaugau
ggcacuugaa 1680agcaaguguu uggauauuca ugcgagcaua caaacuaugg
uagaugguag aaagauuacc 1740acuacagcag gaagauugau uguuaaaucc
aucuugccug auuuugugcc ugaaaauagu 1800uggaauaaag ucuuaaagaa
aaaagacauu gcugcgcuug uagauuaugu uuauaaacaa 1860ggugguuuag
agauuacagc aaguuucuua gauagacuua aaaauuuagg uuuugaauau
1920gcaacuaaag cagguauuuc aauuucgauu gcagauauua uuguuccuaa
ugauaaacaa 1980aaagcuaucg augaagcaaa aaaacaagua agagaaauuc
aaaauucuua uaaucucggu 2040uugauuacuu caggggaaag auacaauaaa
aucauugaua uuuggaaaag uacaaacaau 2100guucuuucaa aagaaaugau
gaagcuugua gaaaaagaua aagaagguuu uaacucuauu 2160uauaugaugg
cagauucugg ugcuaggggu agugcagcuc aaauuucuca gcuugcugcg
2220augagaggac uuaugaccaa accugauggu ucuauuaucg aaacgccuau
uauuucaaau 2280uuccgugaag ggcuaaaugu ucuugaauac uuuauuucaa
cucacggugc uagaaaaggu 2340cuugcagaua ccgcucuuaa aacagcaaau
gcggguuauu ugacaagaaa acucaucgau 2400guugcacaaa auguaaaaau
uaccauugaa gauuguggaa cacaugaggg uguugaaauc 2460aaugaaauua
ccgcagauag uucuauuaua gaaacuuuag aagaaagaau uuuaggcagg
2520guuuuagcug aggaugugau ugauccuauu acaaauucug ugcuuuuugc
ggaagguacu 2580uuaauggaug aggaaaaagc aaaaauucuu ggcgaaagcg
guauaaaaag ugucaauauc 2640cgcacuccua uuaccugcaa agcuaaaaaa
ggaauuugug caaaauguua ugguaucaau 2700cuuggugaag guaaauuagu
aaaaccaggc gaagcagugg gaauuauuuc cgcucaaucu 2760aucggugaac
caggaacgca gcuaacucua agaacuuucc auagcggggg aacugcaagu
2820acggauuuac aagauagaca aguaagcgca caaaaagaag guuuuauaag
auuuuauaau 2880cuuaaaacuu auaaaaacaa agaagguaaa aauaucguag
caaaucguag aaaugcggcg 2940guuuuacuug uggagccaaa aaucaaaacu
ccauuuaaag gugugauuaa uauagaaaau 3000auucaugaag augugauugu
uucuauuaaa gauaaaaaac aagaaguaaa auacauauua 3060agaaaauacg
aucuugcuaa accaaaugaa uuagcaggug uaaguggcag uauagaugga
3120aaacuuuauu ugccauauca aagcggcaug caaguagaag aaaaugaaag
uaucguagaa 3180gugauuaaag aggguuggaa uguaccaaau cguauuccuu
uugcgaguga aauuuuagua 3240gaagauggcg agccuguagu ucaaaauauc
aaagcaggcg aaaaaggaac acucaaauuu 3300uacauccuua aaggcgaugg
uuuagauaga guaaaaaaug uuaaaaaagg ugauauuguu 3360aaagaaaaag
gauucuuugu agugauugcu gaugaaaaug auagagaagc aaaaagacac
3420uauaucccaa gagaaucuaa gauagaauuu aacgauagug aaaaaaucga
ugacgcaaau 3480acuaucauug caagugcucc uaaaaaagaa agaaaaguga
uugcagaaug ggaugcuuau 3540aauaauacua ucauugcaga aauugauggu
guuguaagcu uugaggauau ugaagcaggu 3600uauagugccg augagcaaau
ugaugaagcu acagguaagc guucuuuagu aauuaaugag 3660uauuuaccua
gcggaguuag accaacuuug guaauugcag gaaaagguga uaaagcugug
3720cguuaucacc uugaaccaaa aaccguuauu uuuguucaug auggcgauaa
aauugcucaa 3780gcagauauuu uagcaaaaac uccaaaagca gcagcuaaau
caaaggauau uacaggaggu 3840cuuccaagag uuucugaacu uuuugaagca
agaaaaccaa aaaaugcggc ugugauugca 3900gaaauugaug guguuguucg
uuuugauaag ccuuugcguu cuaaagaaag aauuauuauc 3960caagcagaag
auggaacaag ugcugaguau uuaaucgaca aaucaaaaca uauucaagua
4020agagauggag aguuuauuca ugcaggugaa aaacuuacag auggaguugu
uucgagucau 4080gaugugcuua aaauuuuagg ugaaaaagcc uugcauuauu
auuugauuuc ugaaauucag 4140caaguuuauc gcggacaagg uguugugauu
ucugauaaac auauagaagu uaucguuucu 4200caaaugcuaa gacaaguaaa
aguuguagau aguggacaua cgaaauuuau ugaaggugau 4260uugguuucaa
gacguaaauu ccgugaagaa aaugaaagaa ucauuagaau ggguggagaa
4320ccagcuauug ccgagccugu gcuuuuaggu guaacaagag cagcgauugg
aagugauagu 4380gugauuucug cggcuucauu ccaagaaacu accaaaguuu
uaacugaagc aaguauagca 4440gguaaauuug acuacuuaga agauuuaaaa
gaaaauguua uucuagguag aaugauuccu 4500guuggaacag ggcuuuaugg
cgaacaaaau uuaaaacuua aagaacaaga auaa 45549120DNAArtificial
sequenceSynthetic PCR primer 91gctatcccac aaattgataa 20
* * * * *