U.S. patent application number 14/880534 was filed with the patent office on 2016-02-11 for novel prrs virus inducing type i interferon in susceptible cells.
The applicant listed for this patent is Boehringer Ingelheim Vetmedica GmbH. Invention is credited to Andreas GALLEI.
Application Number | 20160040136 14/880534 |
Document ID | / |
Family ID | 45928625 |
Filed Date | 2016-02-11 |
United States Patent
Application |
20160040136 |
Kind Code |
A1 |
GALLEI; Andreas |
February 11, 2016 |
NOVEL PRRS VIRUS INDUCING TYPE I INTERFERON IN SUSCEPTIBLE
CELLS
Abstract
The present invention relates to the field of attenuated live
viruses useful as vaccine or medicament for preventing or treating
Porcine Reproductive and Respiratory Syndrome (PRRS) in swine, and
is based on the surprising finding of a PRRS virus which is able to
induce the interferon type I response of a cell infected by said
virus. In one embodiment, the PRRS virus according to the invention
is a PRRS virus mutant comprising, in comparison with the genome of
a wild type strain, a mutation in the gene encoding the non
structural protein 1 (nsp1) of said virus.
Inventors: |
GALLEI; Andreas; (Hannover,
DE) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Boehringer Ingelheim Vetmedica GmbH |
Ingelheim am Rhein |
|
DE |
|
|
Family ID: |
45928625 |
Appl. No.: |
14/880534 |
Filed: |
October 12, 2015 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
13558669 |
Jul 26, 2012 |
9187731 |
|
|
14880534 |
|
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Current U.S.
Class: |
424/204.1 ;
424/93.6; 435/235.1; 435/29; 435/320.1; 435/352; 435/6.11;
435/6.12; 435/7.1; 435/7.92; 536/23.72 |
Current CPC
Class: |
A61K 2039/552 20130101;
C12N 2770/10022 20130101; A61K 35/76 20130101; A61P 31/14 20180101;
A61K 39/12 20130101; C12N 7/00 20130101; A61P 37/04 20180101; C12N
2770/10034 20130101; C12N 2770/10021 20130101; C12N 2770/10062
20130101; C07K 14/005 20130101 |
International
Class: |
C12N 7/00 20060101
C12N007/00; A61K 39/12 20060101 A61K039/12; A61K 35/76 20060101
A61K035/76; C07K 14/005 20060101 C07K014/005 |
Foreign Application Data
Date |
Code |
Application Number |
Jul 29, 2011 |
EP |
11176023.7 |
Claims
1. A replication-competent Porcine Reproductive and Respiratory
Syndrome (PRRS) virus comprising a deletion of at least three
consecutive nucleotide triplets in the gene sequence encoding the
N-terminal domain (NTD) of the nsp1.beta. subunit of the nsp1
protein of said virus, wherein the NTD starts with the N-terminal
amino acid sequence SXXY (SEQ ID NO: 60) of nsp1.beta. and ends
with the serine (S) residue within the amino acid sequence SFP (SEQ
ID NO: 62), wherein said deletion of at least three consecutive
nucleotide triplets comprises a deletion of the nucleotide triplet
encoding the first arginine residue (R) located at least 21 amino
acid residues in the C-terminal direction from the N-terminal amino
acid residue of nsp1.beta. and a deletion of the two nucleotide
triplets coding for the two amino acid residues flanking said R
residue in the N-terminal direction, and wherein the modified PRRS
virus induces production and secretion of interferon type I by a
cell infected with said virus.
2. The PRRS virus according to claim 1, wherein said mutation
comprises a deletion of three consecutive nucleotide triplets
coding for the amino acid sequence RXR (SEQ ID NO: 66) of the
nsp1.beta. subunit, or wherein said mutation comprises a deletion
of four consecutive nucleotide triplets coding for the amino acid
residues GRXR (SEQ ID NO: 68) of the nsp1.beta. subunit, wherein X
is a genetically encoded amino acid residue.
3. An immunogenic composition comprising the PRRS virus according
to claim 1.
4. The immunogenic composition according to claim 3, comprising an
amount of 10.sup.1 to 10.sup.7 viral particles per dose
5. The immunogenic composition according to claim 3, comprising an
amount of 10.sup.3 to 10.sup.6 particles per dose.
6. The immunogenic composition according to claim 3, comprising an
amount of 10.sup.4 to 10.sup.6 particles per dose.
7. The immunogenic composition according to claim 3, comprising an
amount of said PRRS virus which is equivalent to a virus titer of
at least about 10.sup.3 TCID50/mL per dose.
8. The immunogenic composition according to claim 3, comprising an
amount of said PRRS virus which is equivalent to a virus titer of
between 10.sup.3 to 10.sup.5 TCID50/mL per dose.
9. The immunogenic composition according to claim 3, further
comprising one or more pharmaceutically acceptable carriers or
excipients.
10. The immunogenic composition according to claim 9, wherein said
one or more pharmaceutically acceptable carriers or excipients are
selected from the group consisting of solvents, dispersion media,
adjuvants, stabilizing agents, diluents, preservatives,
antibacterial and antifungal agents, isotonic agents, and
adsorption delaying agents.
11. The PRRS virus according to claim 1, wherein said PPRS virus
has increased sensitivity to interferon type I.
12. The PRRS virus according to claim 1, wherein said PRRS virus is
an attenuated PRRS virus.
13. The PRRS virus according to claim 1, wherein said PPRS virus is
a genotype I virus.
14. The PRRS virus according to claim 1, wherein said interferon
type I is Interferon-.alpha., and/or Interferon-.beta. or
combinations thereof.
15. The PRRS virus according to claim 1, wherein said cell is a
mammalian cell, and wherein said mammalian cell is a cell of a
primary or secondary cell line or a host cell.
16. The PRRS virus according to claim 15, wherein said mammalian
cell is a porcine cell or a simian cell.
17. The PRRS virus according to claim 16, wherein said porcine cell
is a porcine macrophage.
18. The PRRS virus according to claim 16, wherein said simian cell
is a MA-104 or a MARC-145 cell.
19. A polynucleotide comprising the genome of the PRRS virus
according to claim 1.
20. A DNA Vector comprising a copy of the polynucleotide of claim
19.
21. A virus particle comprising the polynucleotide of claim 19.
22. A cell comprising the polynucleotide of claim 19.
23. A cell comprising the DNA-vector of claim 20.
24. A method of preparing a PRRS virus composition comprising the
cultivation of the PRRS virus according to claim 1 in cell
culture.
25. A method for the treatment of Porcine Reproductive and
Respiratory Syndrome comprising administering an effective amount
of the PRRS virus according to claim 1 to a swine, pig, piglet or a
sow in need of said treatment.
26. A method for inducing an immune response against PRRSV
comprising administering an effective amount of the immunogenic
composition according to claim 1 to a swine, pig, piglet or a sow
in need thereof.
27. A method for the detection of interferon type I and/or the mRNA
coding for interferon type I, by the cell infected with the
modified PRRS virus according to claim 1, wherein the interferon
type I and/or the mRNA coding for the interferon type I is detected
by a bioassay, wherein said bioassay is preferably selected from
the group consisting of ELISA, PCR, GLISA, IFA, biosensoric
measurement, Surface Plasmon Resonance (SPR) measurement, selective
media, lateral flow, biochip measurement, immunomagnetic
separation, electrochemiluminescence, chromogenic media,
immunodiffusion, DNA hybridization, staining, colorimetric
detection, luminescence, and combinations thereof.
28. The method according to claim 24, wherein the immunogenic
composition is administered in a single dose or in two doses.
Description
SEQUENCE LISTING
[0001] The instant application contains a Sequence Listing which
has been submitted in ASCII format via EFS-Web and is hereby
incorporated by reference in its entirety. Said ASCII copy, created
on Oct. 1, 2014 is named 01-2738-SEQ-US-2.txt and is 243,257 bytes
in size.
FIELD OF THE INVENTION
[0002] The present invention belongs to the field of vaccines and
medicaments for the prophylaxis and treatment of infectious
diseases. In particular, it relates to attenuated live viruses
useful as vaccine or medicament for preventing or treating Porcine
Reproductive and Respiratory Syndrome (PRRS), a viral disease
affecting swine.
BACKGROUND OF THE INVENTION
[0003] Porcine reproductive and respiratory syndrome virus (PRRSV)
is a member of the virus family Arteriviridae and belongs, together
with the Coronaviridae, to the virus order Nidovirales. PRRSV is an
enveloped virus with a single-stranded, positive-sense RNA genome
of about 15 kilobases comprising nine open reading frames (ORFs),
namely ORF1a, ORF1ab, ORF2a, ORF 2ab, and ORFs 3 through ORF7.
ORFs1a and 1ab encode large polyproteins that are processed into
the viral nonstructural proteins (nsp) by auto- and transcleavages
of viral proteases nsp1, nsp2, and nsp4 (Snijder and Meulenberg,
1998).
[0004] There are two distinct viral PRRSV genotypes causing similar
clinical symptoms that diverge by about 40% on nucleotide sequence
level, genotype I (EU) and genotype II (US). The North American
(US) prototype strain is VR-2332, while the European (EU) prototype
strain is Lelystad virus.
[0005] PRRSV is considered one of the economically most important
infectious agents in pigs causing late-term reproductive failure in
sows and respiratory disease in growing pigs. Often, PRRSV
infection is complicated by secondary bacterial infections being
attributed to the immunosuppressive nature of the virus. Also,
PRRSV viremia lasts for weeks, and virus then still can be detected
in lymphoid organs for several months, demonstrating difficulties
or failure of the host's immune response to clear the virus
(Allende et al., 2000).
[0006] The specific immune response to PRRSV infection is
characterized by delayed induction of neutralizing antibodies
(Lopez and Osorio, 2004) and short cell-mediated immune response
(Xiao et al., 2004). It is commonly accepted that these effects can
in part be attributed, along with presentation of decoy epitopes
(Ostrowski et al., 2002; Ansari et al., 2006) and glycan shielding
of viral envelope proteins (Ansari et al., 2006), to the viral
inhibition of the host's innate immune system. It has been
demonstrated that PRRSV infection does not or only weakly or
delayedly induce production of type I interferon (IFN) (interferon
.alpha. and interferon .beta.; (Miller et al., 2004)) or type II
IFN, (interferon .alpha.; (Meier et al., 2003)) in susceptible cell
lines (swine pulmonary alveolar macrophages, monkey kidney cells
MARC-145) and/or pigs (Buddaert et al., 1998).
[0007] Thus, there is a need for novel vaccines and medications
effecting a rapid induction of neutralizing antibodies and
interferon responses for the prophylaxis and treatment of PRRSV
infection.
BRIEF DESCRIPTION OF THE FIGURES
[0008] FIG. 1: Sequence alignment of nsp1.beta. protein sequences
from parental EU PRRSV strain LoN94-13 and from vaccine
candidates.
[0009] FIG. 2: Sequence alignment of nsp1.beta. proteins from PRRSV
strains.
[0010] FIG. 3A: PRRSV-specific immunofluorescence of virus stock
titrations of delta nsp1 XVII-1.Black, negative fluorescence; light
grey, few positive cells; grey, foci of positive cells; dark grey,
complete cell monolayer positive.
[0011] FIG. 3B: PRRSV-specific immunofluorescence of virus stock
titrations of delta nsp1 XVIII-12. Black, negative fluorescence;
light grey, few positive cells; grey, foci of positive cells; dark
grey, complete cell monolayer positive.
[0012] FIG. 4: Virus stocks of vaccine candidates contain
IFN.beta..
[0013] FIG. 5: Time course of IFN.beta. induction after infection
of MA104 cells.
[0014] FIG. 6: Delta nsp1 mutants show increased sensitivity to
type I IFN.
[0015] FIG. 7: Mean body temperatures of vaccinated groups before
and after challenge.
DETAILED DESCRIPTION
[0016] IFNs play an important role in establishing an effective
adaptive immune response against viral infections, and many viruses
therefore have developed strategies and counteractions against
onset of the host's innate immune system (Haller and Weber, 2009).
In the interest to identify the anticipated PRRSV IFN
antagonist(s), extensive screening analyses based on cell lines
stably expressing genes of interest or on cells transfected with
protein-expressing plasmids have identified several PRRSV
nonstructural proteins (nsps) including nsp1 (see below), nsp2
(Beura et al., 2010; Li et al., 2010), nsp4 (Beura et al., 2010),
and nsp11 (Beura et al., 2010; Shi et al., 2011a) to be involved in
blocking the induction of type I IFN.
[0017] nsp1 is located at the N-terminus of the PRRSV ORF1a-derived
polyprotein 1a and is processed into two multifunctional subunits,
nsp1.alpha. and nsp1.beta., each of which contains a papain-like
cystein protease (PCP) domain essential for self-release from the
viral polyprotein (den Boon et al., 1995; Chen et al., 2010).
nsp1.alpha. contains an N-terminal zinc-finger domain and the
PCP.alpha. protease domain, while nsp1.beta. contains PCP.beta..
For both nsp1 subunits, nsp1.alpha. and nsp1.beta., the
three-dimensional crystal structure has been resolved (Sun et al.,
2009; Xue et al., 2010). According to these analyses, nsp1.beta.
consists of an N-terminal domain (NTD), a linker domain (LKD), the
PCP domain (PCP beta), and a C-terminal extension (CTE); (Xue et
al., 2010), see FIG. 2. C-terminal, nsp1.beta.-mediated cleavage of
nsp1 from nsp2 occurs at site WYG/AGR for PRRSV US strains (Kroese
et al., 2008) or is predicted at site WYG/AAG for PRRSV EU strains
(Chen et al., 2010), while nsp1.alpha./nsp1.beta. cleavage occurs
at site ECAM/AxVYD for PRRSV US strains or is predicted at site
EEAH/SxVYR for PRRSV EU strains (Chen et al., 2010).
[0018] Several studies on protein level demonstrated to the
mechanistic detail that PRRSV nsp1 and/or its autocleavage-derived
subunits nsp1.alpha. and/or nsp1.beta. inhibit type I IFN
production by interfering with IFN transcription (Song et al.,
2010; Kim et al., 2010; Chen et al., 2010; Beura et al., 2010). In
addition, it has been demonstrated that nsp1.beta. interferes with
the cellular response to interferon (interferon signaling); (Chen
et al., 2010). Moreover, it was demonstrated that PRRSV infection
inhibits IFN.alpha. and/or IFN.beta. production in PRRSV infected
cells in vitro (Kim et al., 2010; Beura et al., 2010), the
subcellular localization of nsp1(subunits) was determined (Song et
al., 2010; Chen et al., 2010), and mechanistic aspects of type I
IFN inhibition that were obtained by others from single protein
expression experiments were confirmed in cells infected with PRRSV
(Shi et al., 2010). Finally, a nsp1 mutagenesis study based on nsp1
protein expression investigated effects on viral IFN inhibition
(Shi et al., 2011b), showing that mutations that inactivated
papain-like cysteine protease activity of nsp1.alpha. made nsp1
lose its IFN antagonism activity, whereas mutations that
inactivated papain-like cysteine protease activity of nsp1.beta.
did not influence the IFN antagonism activity of nsp1.
[0019] However, a viable PRRSV strain that induces IFN production
and/or does not interfere with IFN signaling after infection of
susceptible cells and/or the host, in particular a PRRSV strain
comprising a genomic mutation in its nsp1 gene, has not been
described yet.
[0020] It is thus an aim of the present invention to make available
such a viable PRRS virus inducing the IFN response of a cell, in
particular of a host cell, wherein said PRRS virus may serve as an
effective vaccine or medicament for the prophylaxis or treatment of
the Porcine reproductive and respiratory syndrome in swine.
[0021] The solution to the above technical problem is achieved by
the description and the embodiments characterized in the
claims.
[0022] Thus, the invention in its different aspects and embodiments
is implemented according to the claims.
[0023] The invention is based on the surprising finding of a
Porcine Reproductive and Respiratory Syndrome (PRRS) virus which is
able to induce the interferon type I response of a cell, in
particular of a host cell.
[0024] Hence, one aspect of the invention concerns a PRRS virus
(PRRSV) which is able to induce the interferon type I production
and secretion by a cell infected by said virus, wherein the PRRS
virus according to the invention in particular is able to induce or
induces the interferon type I production and secretion by an
interferon competent cell infected by said virus.
[0025] As used herein, it is understood that the terms "interferon
type I", "IFN type I", "type I interferon" and "type I IFN" are
equivalent.
[0026] Interferon type I production and secretion by a cell, as
described herein, particularly means that interferon type I is made
and released by a cell in response to the infection of the PRRS
virus according to the invention. In this regard, interferon type
I, as mentioned herein, is preferably interferon-.alpha. and/or
interferon-.beta..
[0027] The infection of a cell by the PRRS virus according to the
invention in particular includes attachment of the virus to the
cell, entry of the virus into the cell, disassembly of the virion,
and preferably replication and transcription of the viral genome,
expression of viral proteins, and assembly and release of new
infectious viral particles.
[0028] The cell, as mentioned herein, is a primary or secondary
susceptible cell, preferably a mammalian cell, in particular a
porcine or a simian cell, more preferably said cell is a porcine
macrophage or a MA-104 cell or a MARC-145 cell or a Vero cell.
[0029] It is further understood that the PRRS virus according to
the invention is able to induce in vitro and/or in vivo the
interferon type I production and secretion by a cell infected by
said virus.
[0030] Preferably, the PRRS virus according to the invention is a
live PRRS virus and/or a modified-live PRRS virus and/or an
attenuated PRRS virus.
[0031] The term "attenuated PRRS virus", as described herein, is in
particular directed to a PRRS virus which is attenuated in vitro
and/or in vivo, more particular in susceptible cell lines and/or
the host.
[0032] The term "host", as used herein, is in particular directed
to animals infectable with PRRS virus, in particular swine, more
particular pigs, such as domestic pigs.
[0033] As mentioned herein, "attenuated" particularly relates to a
reduced virulence of a pathogen, in particular of a wild type PRRS
virus, wherein "virulence" is understood to be the degree of
pathogenicity, and wherein "pathogenicity" is directed to the
ability of the pathogen to produce clinical symptoms in the host,
such as elevated body temperature.
[0034] In particular preferably, the PRRS virus according to the
invention has or shows increased sensitivity to type I INF when
compared to wild type PRRSV, wherein the term "sensitivity to type
I INF" is understood as reduced viral infectivity when IFN.beta. is
present, preferably in a sufficient amount for significantly
reducing viral infectivity of wild type PRRSV, in the medium
surrounding the virus at the time of infection.
[0035] The term "wild type PRRS virus" or "wild type PRRSV",
respectively, as used herein, is in particular directed to an
infectious pathogenic PRRS virus, which is particularly capable of
causing PRRS in swine. In one particular preferred embodiment, the
term "wild type PRRS virus" is directed to a PRRS virus whose
genome comprises a RNA sequence or consists of a RNA
polynucleotide, wherein said RNA sequence or RNA polynucleotide is
a RNA copy of a polynucleotide selected from the group consisting
of SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15.
[0036] In one embodiment, the PRRS virus according to the invention
is a PRRS virus mutant, in particular comprising, in comparison
with the genome of a wild type PRRSV strain, a mutation in a gene
encoding a protein of said virus.
[0037] In a preferred embodiment, the PRRS virus according to the
invention comprises a mutation in the gene encoding the nsp1
protein of said virus. Thus, the invention preferably concerns a
PRRS virus which is able to induce the interferon type I production
and secretion by an infected cell as a result of a mutation in the
gene encoding the nsp1 protein, wherein said mutation is preferably
a mutation as mentioned hereinafter.
[0038] Hence, the invention particularly concerns a PRRS virus
comprising, in comparison with the genome of a wild type strain, a
mutation in the gene encoding the nsp1 protein. Accordingly, the
mutation as described herein is preferably a mutation in comparison
with the sequence of the corresponding wild type gene encoding the
nsp1 protein.
[0039] The invention is thus preferably directed to a PRRS virus;
in particular a wild type PRRS virus, wherein a mutation,
preferably a mutation as mentioned hereinafter, has been
implemented in the genome of said virus resulting in a non-natural
nsp1 protein or in the lack of nsp1 protein of said virus.
[0040] In the context of the invention it is understood, that the
mutation as described herein may be implemented to any PRRS virus,
such as to a PRRS virus selected from the group consisting of wild
type PRRS virus, attenuated PRRS virus, modified-life PRRS virus,
PRRS virus mutant and combinations thereof. In one example, the
mutation as described herein may be implemented to an attenuated
and/or live PRRS virus, for example to a PRRS virus selected from
the group of the PRRS virus strains that have been deposited on 27
Oct. 2004 with the European Collection of Cell Cultures (ECACC),
Porton Down, Salisbury, Wiltshire, SP4 OJG, Great Britain, under
the Accession Numbers ECACC 04102703, ECACC 04102702, and ECACC
04102704. Hence, the mutation as described herein can be combined
with one or more other mutations, preferably one ore more other
attenuating mutations, in a PRRS virus.
[0041] In the context of the invention, the term "PPRRS virus" is
in particular equivalent with "PRRS virus strain".
[0042] The term "mutation" in the context of the invention is
understood as a change in a genomic sequence, in particular in the
RNA sequence of a PRRS virus. Since viruses that use RNA as their
genetic material have rapid mutation rates, the term "mutation", as
mentioned herein, is particularly directed to a genetically
engineered change in a genomic sequence, such as by site directed
mutagenesis, which in particular results in a virus growing to
titers significantly lower than wild type PRRS virus in interferon
competent cells and/or in the infected host, when propagated under
the same conditions. Moreover, in another preferred embodiment the
mutation described herein can also be caused by natural mutation
and subsequent isolation of the PRRS virus according to the
invention, wherein said isolated virus includes the mutation
described herein.
[0043] Preferably, the mutation, as described herein, comprises or
consists of one or more point mutations and/or one or more genomic
deletions and/or one or more insertions.
[0044] The term "nsp1 protein", as used herein, is directed to the
PRRSV nonstructural protein 1.
[0045] The nsp1 protein is preferably a polypeptide having at least
70%, preferably at least 80%, more preferably at least 90%, still
more preferably at least 95% or in particular 100% sequence
identity with a polypeptide selected from the group consisting of
SEQ ID NO:1 and SEQ ID NO:2 if the PRRS virus according to the
invention is a genotype I PRRS virus, or the nsp1 protein is
preferably a polypeptide having at least 70%, preferably at least
80%, more preferably at least 90%, still more preferably at least
95% or in particular 100% sequence identity with the polypeptide
set forth in SEQ ID NO:3 if the PRRS virus according to the
invention is a genotype II PRRS virus.
[0046] In one aspect, the PRRS virus according to the invention
preferably also may comprise a mutation in the gene encoding the
nsp1 protein selected from the group consisting of SEQ ID NOs
1-3.
[0047] It is hence understood that the PRRS virus according to the
invention is a genotype I PRRS virus or a genotype II PRRS virus.
It is further understood that the terms "genotype I" and "genotype
II" are equivalent to the terms "genotype 1" and "genotype 2" or to
the terms "type 1" and "type 2", as frequently used in the
literature in the context of PRRSV.
[0048] Sequence identity in the context of the invention is
understood as being based on pairwise determined similarity between
nucleotide or protein sequences. The determination of percent
identity between two sequences is preferably accomplished using a
mathematical algorithm, in particular the well-known Smith-Waterman
algorithm (Smith and Waterman, M. S. (1981) J Mol Biol,
147(1):195-197). For purposes of the present invention, percent
sequence identity of an amino acid sequence is determined using the
Smith-Waterman homology search algorithm using an affine 6 gap
search with a gap open penalty of 12 and a gap extension penalty of
2, BLOSUM matrix 62. The Smith-Waterman homology search algorithm
is taught in Smith and Waterman (1981) Adv. Appl. Math 2:482-489,
herein incorporated by reference. A variant may, for example,
differ from the reference nsp1, nsp1.alpha., nsp1.beta. or NTD
molecule by as few as 1 to 15 amino acid residues, as few as 1 to
10 amino acid residues, such as 6-10, as few as 5, as few as 4, 3,
2, or even 1 amino acid residue. Alternatively, percent identity of
a nucleotide sequence is determined using the Smith-Waterman
homology search algorithm using a gap open penalty of 25 and a gap
extension penalty of 5. Such a determination of sequence identity
can be performed using, for example, the DeCypher Hardware
Accelerator from TimeLogic Version G, or the sequence identity is
determined with the software CLC MAIN WORKBENCH 4.1.1 (CLC
BIO).
[0049] The term "having 100% sequence identity", as used herein, is
understood to be equivalent to the term "being identical".
[0050] In another preferred embodiment, the mutation in the PRRS
virus according to the invention comprises or consists of one or
more point mutations and/or one or more genomic deletions and/or
one or more insertions.
[0051] In a further preferred embodiment, the PRRS virus according
to the invention comprises a mutation in the gene sequences
encoding the nsp1.alpha. subunit of the nsp1 protein and/or the
nsp1.beta. subunit of the nsp1 protein of said virus.
[0052] The term "nsp1.alpha.", as mentioned herein, is thus
directed to the PRRSV nsp1.alpha. subunit of the nsp1 protein, and
the term "nsp1.beta.", as used herein, is hence directed to the
PRRSV nsp1.beta. subunit of the nsp1 protein.
[0053] The nsp1.alpha. is preferably a polypeptide having at least
70%, preferably at least 80%, more preferably at least 90%, still
more preferably at least 95% or in particular 100% sequence
identity with a polypeptide selected from the group consisting of
SEQ ID NO:4 and SEQ ID NO:5 if the PRRS virus according to the
invention is a genotype I PRRS virus, or the nsp1.alpha. is
preferably a polypeptide having at least 70%, preferably at least
80%, more preferably at least 90%, still more preferably at least
95% or in particular 100% sequence identity with the polypeptide
set forth in SEQ ID NO:6 if the PRRS virus according to the
invention is a genotype II PRRS virus.
[0054] The nsp1.beta. is preferably a polypeptide having at least
70%, preferably at least 80%, more preferably at least 90%, still
more preferably at least 95% or in particular 100% sequence
identity with a polypeptide selected from the group consisting of
SEQ ID NO:7 and SEQ ID NO:8 if the PRRS virus according to the
invention is a genotype I PRRS virus, or the nsp1.beta. is
preferably a polypeptide having at least 70%, preferably at least
80%, more preferably at least 90%, still more preferably at least
95% or in particular 100% sequence identity with the polypeptide
set forth in SEQ ID NO:9 if the PRRS virus according to the
invention is a genotype II PRRS virus.
[0055] In a particular preferred embodiment, the PRRS virus
according to the invention comprises a mutation in the gene
sequence encoding the N-terminal domain (NTD) of the nsp1.beta. of
said virus. The term "NTD", as mentioned herein, is thus directed
to the N-terminal domain of the PRRSV nsp1.beta. subunit.
[0056] The NTD is preferably a polypeptide or has a polypeptide
sequence, respectively, having at least 60%, particularly at least
70%, preferably at least 80%, more preferably at least 90%, still
more preferably at least 95% or in particular 100% sequence
identity with a polypeptide or a polypeptide sequence,
respectively, selected from the group consisting of SEQ ID NO:10
and SEQ ID NO:11 if the PRRS virus according to the invention is a
genotype I PRRS virus, or the NTD is preferably a polypeptide or
has polypeptide sequence, respectively, having at least 60%,
particularly at least 70%, preferably at least 80%, more preferably
at least 90%, still more preferably at least 95% or in particular
100% sequence identity with the polypeptide or the polypeptide
sequence, respectively, set forth in SEQ ID NO:12 if the PRRS virus
according to the invention is a genotype II PRRS virus.
[0057] In a preferred embodiment, the NTD comprising the mutation
has the sequence selected from the group consisting of the
sequences
TABLE-US-00001 SXXYXXXXXVXFXDXXXXGXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXXGXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
as set forth in SEQ ID NOs:36-41, if the PRRS virus according to
the invention is a genotype I PRRS virus, or the NTD comprising the
mutation preferably has the sequence selected from the group
consisting of the sequences
TABLE-US-00002 AXVYDIGXXAVMXVAXXXXSWAGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXXSWGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXXSGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXXGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXGGXXXXFXXXXXXLXLXAXXXXXS,
as set forth in SEQ ID NOs: 42-47, if the PRRS virus according to
the invention is a genotype II PRRS virus.
[0058] Thus, the PRRS virus according to the invention preferably
comprises a gene sequence encoding a NTD selected from the
sequences
TABLE-US-00003 SXXYXXXXXVXFXDXXXXGXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXXGXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
SXXYXXXXXVXFXDXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,
as set forth in SEQ ID NOs:36-41, if the PRRS virus according to
the invention is a genotype I PRRS virus, or the PRRS virus
according to the invention preferably comprises a gene sequence
encoding a NTD selected from the sequences
TABLE-US-00004 AXVYDIGXXAVMXVAXXXXSWAGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXXSWGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXXSGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXXGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXXGGXXXXFXXXXXXLXLXAXXXXXS,
AXVYDIGXXAVMXVAXXGGXXXXFXXXXXXLXLXAXXXXXS,
as set forth in SEQ ID NOs: 42-47, if the PRRS virus according to
the invention is a genotype II PRRS virus.
[0059] In one exemplary embodiment, the NTD comprising the mutation
may, for instance, have the sequence selected from the group
consisting of the sequences
TABLE-US-00005 SSVYRWKKFVVFTDSSXNGRMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSXNGMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSXNMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSXMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSMMWTPESDDSAXLEXLPPELERQVEILIRS,
as set forth in SEQ ID NOs:48-53, if the PRRS virus according to
the invention is a genotype I PRRS virus, or the NTD comprising the
mutation may, for instance, have the sequence selected from the
group consisting of the sequences
TABLE-US-00006 ATVYDIGXXAVMYVAXXKVSWAGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKVSWGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKVSGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKVGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXGGXEVKFEXVPXELKLXANRLXTS,
as set forth in SEQ ID NOs: 54-59, if the PRRS virus according to
the invention is a genotype II PRRS virus.
[0060] Thus, in one exemplary embodiment, the PRRS virus according
to the invention may, for instance, comprise a gene sequence
encoding a NTD selected from the sequences
TABLE-US-00007 SSVYRWKKFVVFTDSSXNGRMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSXNGMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSXNMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSXMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSSMMWTPESDDSAXLEXLPPELERQVEILIRS,
SSVYRWKKFVVFTDSMMWTPESDDSAXLEXLPPELERQVEILIRS,
as set forth in SEQ ID NOs:48-53, if the PRRS virus according to
the invention is a genotype I PRRS virus, or the PRRS virus
according to the invention may, for instance, comprise a gene
sequence encoding a NTD selected from the sequences
TABLE-US-00008 ATVYDIGXXAVMYVAXXKVSWAGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKVSWGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKVSGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKVGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXKGGXEVKFEXVPXELKLXANRLXTS,
ATVYDIGXXAVMYVAXXGGXEVKFEXVPXELKLXANRLXTS,
as set forth in SEQ ID NOs: 54-59, if the PRRS virus according to
the invention is a genotype II PRRS virus.
[0061] It is thus understood that a genotype I PRRS virus, as
mentioned herein, is in particular a virus whose genome comprises a
gene sequence coding for a polypeptide having at least 70%,
preferably at least 80%, more preferably at least 90%, still more
preferably at least 95% or in particular 100% sequence identity
with a polypeptide selected from the group consisting of SEQ ID
NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7 and SEQ ID
NO:8, or is in particular a virus whose genome comprises a gene
sequence coding for a polypeptide having at least 60%, particularly
at least 70%, preferably at least 80%, more preferably at least
90%, still more preferably at least 95% or in particular 100%
sequence identity with a polypeptide selected from the group
consisting of SEQ ID NO:10 and SEQ ID NO:11.
[0062] Preferably, the genotype I PRRS virus, as mentioned herein,
comprises or consists of a RNA polynucleotide having at least 70%,
preferably at least 80%, more preferably at least 90%, still more
preferably at least 95% or in particular 100% sequence identity
with a RNA polynucleotide complementary to a polynucleotide
selected from the group consisting of SEQ ID NO: 13 and SEQ ID NO:
14.
[0063] It is further understood that a genotype II PRRS virus, as
mentioned herein, is in particular a virus whose genome comprises a
gene sequence coding for a polypeptide having at least 70%,
preferably at least 80%, more preferably at least 90%, still more
preferably at least 95% or in particular 100% sequence identity
with a polypeptide selected from the group consisting of SEQ ID
NO:3, SEQ ID NO:6 and SEQ ID NO:9, or is in particular a virus
whose genome comprises a gene sequence coding for a polypeptide
having at least 60%, particularly at least 70%, preferably at least
80%, more preferably at least 90%, still more preferably at least
95% or in particular 100% sequence identity with the polypeptide
set forth in SEQ ID NO:12.
[0064] Preferably, the genotype II PRRS virus, as mentioned herein,
comprises or consists of a RNA polynucleotide having at least 70%,
preferably at least 80%, more preferably at least 90%, still more
preferably at least 95% or in particular 100% sequence identity
with a RNA polynucleotide complementary to the polynucleotide set
forth in SEQ ID NO: 15.
[0065] In the following, the single letter code for amino acids is
used for amino acid sequences, where additionally X signifies any
genetically encoded amino acid residue.
[0066] Within the context of the invention it is in particular
understood that the N-terminal domain (NTD) of nsp1.beta. starts
with the N-terminus of nsp1.beta., preferably starts with the amino
acid sequence SXXY if the PRRS virus is a genotype I PRRS virus or
with the amino acid sequence AXVY if the PRRS virus is a genotype
II PRRS virus, and the NTD ends with the serine residue (S) within
the amino sequence SFP of the nsp1.beta.. Regarding the NTD domain
of a genotype II PRRS virus it is in particular referred to the
publication Xue et al. 2010, hereby incorporated by reference. Said
amino acid sequences SXXY or AXVY and SFP are especially conserved
in the nsp1.beta. of wild type PRRS viruses and are thus preferably
used amino acid sequence motifs for defining the NTD domain
according to the invention.
[0067] In a particular preferred embodiment, the PRRS virus
according to the invention thus comprises a mutation in the gene
sequence coding for the NTD, wherein the NTD preferably starts with
motif SXXY for PRRS virus type 1 strains or with motif AXVY for
PRRS virus type 2 strains and ends with the serine residue (S)
within the conserved SFP motif of the nsp1.beta. subunit.
[0068] According to the invention, it is in particular preferred if
said mutation is located within the part of the gene sequence
coding for the NTD, where the amino acid stretches folding into B
sheet secondary structures are encoded.
[0069] In one preferred aspect, the mutation, as described herein,
thus comprises a deletion or replacement of a nucleotide triplet
coding for an amino acid residue located within the first 24
N-terminal amino acid residues of the NTD sequence.
[0070] In particular, it is preferred if the mutation described
herein comprises of a deletion or replacement of 2 to 7 or more,
preferably consecutive, nucleotide triplets each coding for an
amino acid residue located within the first 24 N-terminal amino
acid residues of the NTD sequence.
[0071] In another preferred aspect, the mutation, as mentioned
herein, comprises the deletion or replacement of a nucleotide
triplet coding for an amino acid residue with a charged, preferably
positively charged, side chain, in particular coding for an
arginine residue.
[0072] Exemplarily, the mutation mentioned herein preferably
consists of a deletion of 2, 3, 4, 5, 6 or 7 consecutive nucleotide
triplets coding for the respective number (2, 3, 4, 5, 6 or 7) of
consecutive amino acid residues located within the first 24
N-terminal amino acid residues of the NTD sequence, wherein this
mutation comprises the deletion of a nucleotide triplet coding for
an amino acid residue with a charged, preferably positively
charged, side chain, in particular coding for an arginine
residue.
[0073] The mutation, as described herein, preferably comprises or
consists of a deletion or replacement of the nucleotide triplet
coding for the first arginine residue (R) located at least 21 amino
acid residues in C-terminal direction from the N-terminal amino
acid residue of the nsp1.beta. NTD and/or the mutation, as
mentioned herein, comprises a deletion or replacement of the
nucleotide triplet coding for the arginine residue (R) of the nsp1
amino acid sequence RXMW if the PRRS virus is a genotype I PRRS
virus or with the amino acid sequence RGG of said virus if the PRRS
virus is a genotype II PRRS virus and/or the mutation, as mentioned
herein, comprises or consists of a deletion or replacement of the
nucleotide triplet coding for the arginine residue (R) of the amino
acid sequence RMM or RGG of the nsp1 protein.
[0074] According to the invention, the phrase "replacement of a
nucleotide triplet" is understood as being a replacement of a
nucleotide triplet of the wild type sequence by another nucleotide
triplet coding for a different amino acid residue than the wild
type sequence.
[0075] The phrase "deletion of nucleotide triplet" in the context
of the invention is directed to a deletion of a nucleotide triplet
resulting in the deletion of an amino acid residue in comparison
with the wild type sequence.
[0076] In particular, said mutation further comprises a deletion or
replacement of one nucleotide triplet coding for the amino acid
residue flanking said arginine residue (R) in N-terminal direction,
wherein said mutation preferably comprises or consists of a
deletion or replacement of two consecutive nucleotide triplets
coding for the amino acid residues PR.
[0077] More particular, said mutation further comprises a deletion
or replacement of two, three, four, five, six, seven or more
consecutive nucleotide triplets coding for two, three, four, five,
six, seven or more amino acid residues flanking said arginine
residue (R) in N-terminal direction.
[0078] Preferably, said mutation comprises a deletion or
replacement of three consecutive nucleotide triplets coding for the
amino acid sequence RXR or APR of the nsp1.beta., or wherein said
mutation comprises a deletion or replacement of four consecutive
nucleotide triplets coding for the amino acid residues GRXR or WAPR
of the nsp1.beta..
[0079] As result, the PRRS virus according to the invention
comprises in one embodiment a gene coding for a nsp1 protein
selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17,
SEQ ID NO:18, SEQ ID NO:19 and SEQ ID NO:20, or comprises in
another embodiment a gene sequence coding for a nsp1.beta. selected
from the group consisting of SEQ ID NO:21, SEQ ID NO:22, SEQ ID
NO:23, SEQ ID NO:24 and SEQ ID NO:25, or comprises in a further
embodiment a gene sequence coding for a NTD selected from the group
consisting of SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID
NO:29 and SEQ ID NO:30.
[0080] In yet another preferred embodiment, the mutation, as
described herein comprises a deletion of the gene sequence encoding
the (whole) nsp1 protein, encoding the (whole) nsp1.alpha. subunit,
encoding the (whole) nsp1.beta. subunit, or encoding a part,
preferably at least seven consecutive amino acid residues, of or
the (whole) NTD domain of the nsp1.beta. subunit.
[0081] Yet another aspect of the invention is directed to a
polynucleotide comprising or consisting of the genome of the PPRS
virus according to the invention.
[0082] Further, the invention is also directed to a virus particle,
wherein said virus particle comprises a polynucleotide which
comprises or consists of the genome of the PPRS virus according to
the invention or which comprises or consists of a DNA copy of the
PPRS virus according to the invention.
[0083] Still further, the present invention provides a DNA-Vector
comprising a copy of, or a cDNA sequence complementary to,
respectively, a polynucleotide which comprises or consists of the
genome of the PPRS virus according to the invention.
[0084] In one exemplary and non-limiting example the DNA vector, as
mentioned herein, comprises a polynucleotide sequence selected from
the group consisting of SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33,
SEQ ID NO:34 and SEQ ID NO:35.
[0085] Also, the present invention provides a cell comprising a
polynucleotide, which comprises or consists of the genome of the
PPRS virus according to the invention or which comprises or
consists of a DNA copy of the PPRS virus according to the
invention.
[0086] In one exemplary and non-limiting example the PRRS virus
according to the invention, or the genome of the PRRS virus
according to the invention, respectively, comprises a RNA sequence
or consists of a RNA polynucleotide, wherein said RNA sequence or
RNA polynucleotide is a RNA copy of a polynucleotide selected from
the group consisting of SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO:
33, SEQ ID NO: 34 and SEQ ID NO: 35.
[0087] Another aspect of the invention concerns the PRRS virus
according to the invention for use as vaccine or medicament, in
particular for use in the prophylaxis or treatment of Porcine
Reproductive and Respiratory Syndrome preferably in swine. The
present invention further provides a composition containing the
PRRS virus according to the invention for use as a vaccine or as a
medicament, in particular for use in the prophylaxis or treatment
of Porcine Reproductive and Respiratory Syndrome preferably in
swine.
[0088] In a preferred aspect, said vaccine or medicament is
administered in two doses or preferably in one dose to an animal in
need thereof. By the term "prophylaxis or therapy", as mentioned
herein, it is meant that the prophylaxis is to administer a drug,
in particular a vaccine, before exposure to PRRSV and the treatment
is to administer a drug, in particular a medicament, after
infection with PRRSV or onset of PRRS.
[0089] In particular, the vaccine, as mentioned herein, is a live
vaccine and/or a modified live vaccine and/or an attenuated live or
attenuated modified live vaccine.
[0090] The strains of the PRRS virus according to the invention can
be grown and harvested by methods known in the art, e.g. by
propagating in suitable cells like the simian cell line MA-104,
Vero cells, or porcine alveolar macrophages. Preferably, vaccines
according to the present invention are modified live vaccines
comprising one or more of these strains alive in a suitable
carrier, but inactivated virus may also be used to prepare killed
vaccine (KV). Modified live vaccines (MLV) are typically formulated
to allow administration of 10.sup.1 to 10.sup.7 viral particles per
dose, preferably 10.sup.3 to 10.sup.6 particles per dose, more
preferably 10.sup.4 to 10.sup.6 particles per dose (4.0-6.0
log.sub.10 TCID.sub.50). KV may be formulated based on a
pre-inactivation titre of 10.sup.3 to 10.sup.10 viral particles per
dose. The vaccine may comprise a pharmaceutically acceptable
carrier, for example a physiological salt-solution. The vaccine may
or may not comprise an adjuvant. A suitable adjuvant may optionally
also be used. For example, a suitable adjuvant that may be used
a-tocopherol acetate which may be obtained under the trade name
DILUVAC FORTE.RTM.. Alternatively, alum based adjuvants may be
used.
[0091] A further aspect of the invention is thus directed to the
use of the PRRS virus according to the invention for the
preparation of a vaccine or medicament for the prophylaxis or
treatment of Porcine Reproductive and Respiratory Syndrome,
preferably in swine.
[0092] A vaccine according to the present invention may be
presented in form of a freeze-dried preparation of the live virus,
to be reconstituted with a solvent, to result in a solution for
injection. The solvent may, for example, be water, physiological
saline, a buffer, or an adjuvanting solvent. The solvent may
additionally use adjuvants, for example a-tocopherol acetate. The
reconstituted vaccine may then be injected into a pig, for example
as an intramuscular or intradermal injection into the neck. For
intramuscular injection, a volume of 2 ml may be applied, for an
intradermal injection it is typically 0.2 ml. In a further aspect,
the present invention therefore is a product, comprising in
separate containers a freeze-dried composition of the virus, and a
solvent for reconstitution, and optionally further containing a
leaflet or label comprising instructions of use.
[0093] Yet another aspect of the invention thus concerns a method
for the prophylaxis or treatment of Porcine Reproductive and
Respiratory Syndrome comprising administering the PRRS virus
according to the invention to an animal, preferably to a swine,
more preferably to a pig, in particular preferably to a piglet or a
sow.
[0094] A vaccine according to the present invention may not only
comprise the PRRS virus according to the invention as antigen, but
may include further components active against PRRS or other porcine
viral or bacterial diseases, like porcine circovirus, classical
swine fever virus or mycoplasma hyorhinis. Therefore, the invention
further relates to a vaccine as described, characterized in that it
contains at least one further antigen active against a porcine
disease which is not PRRS.
[0095] Still another aspect of the invention thus concerns a
medicament or vaccine comprising the PRRS virus according to the
invention.
[0096] Still a further aspect of the invention is directed to a
method of preparing a vaccine or medicament, in particular for the
prophylaxis or treatment of Porcine Reproductive and Respiratory
Syndrome preferably in swine, said method comprising the
cultivation of the PRRS virus according to the invention in cell
culture.
[0097] Moreover, the invention comprises a method of producing the
PRRS virus according to the invention, said method comprising the
mutagenesis of the gene coding for the nsp1 protein in the genome
of a PRRS virus, and wherein the mutation, as described herein, is
in particular introduced by genetic engineering in the genome of
said virus, preferably by site directed mutagenesis.
[0098] Preferably, for any of the aforementioned methods, the
above-mentioned DNA-vector is used and/or said methods comprise the
detection of interferon type I, in particular interferon type I
secretion, and/or of mRNA coding for interferon type I. Said
detection in particular comprises the detection of interferon type
I and/or of mRNA coding for interferon type I by a bioassay,
wherein said bioassay is preferably selected from the group
consisting of ELISA, PCR, GLISA, IFA, biosensoric measurement,
Surface Plasmon Resonance (SPR) measurement, selective media,
lateral flow, biochip measurement, immunomagnetic separation,
electrochemiluminescence, chromogenic media, immunodiffusion, DNA
hybridization, staining, colorimetric detection, luminescence, and
combinations thereof.
[0099] In yet a further aspect, the invention provides an
immunogenic composition containing the PRRS virus according to the
invention.
[0100] As used herein, the term "immunogenic composition" in
particular refers to a composition that will elicit an immune
response in an animal that has been exposed to the composition. An
immune response may include induction of antibodies and/or
induction of a T-cell response.
[0101] Usually, an "immune response" includes but is not limited to
one or more of the following effects: the production or activation
of antibodies, B cells, helper T cells, suppressor T cells, and/or
cytotoxic T cells, directed specifically to an antigen or antigens
included in the composition or vaccine of interest. Preferably, the
host will display either a therapeutic or a protective
immunological (memory) response such that resistance to new
infection will be enhanced and/or the clinical severity of the
disease reduced. Such protection will be demonstrated by either a
reduction in number or severity of, or lack of one or more of the
symptoms associated with the infection of the pathogen, in the
delay of onset of viremia, in a reduced viral persistence, in a
reduction of the overall viral load and/or in a reduction of viral
excretion. Thus, an "immune response" in particular means but is
not limited to the development in a subset of a cellular and/or
antibody-mediated immune response to the composition or vaccine of
interest.
[0102] In one aspect, the immunogenic composition of the invention
is preferably a vaccine or medicament.
[0103] As used herein, the term "viremia" is particularly
understood as a condition in which PRRS virus particles circulate
in the bloodstream of an animal.
[0104] The term "animal", as mentioned herein, is in particular
directed to swine, more particular to a pig, preferably a domestic
pig.
[0105] In a preferred aspect, the immunogenic composition of the
invention comprises an amount of 10.sup.1 to 10.sup.7 viral
particles of the PRRS virus according to the invention per dose,
preferably 10.sup.3 to 10.sup.6 particles per dose, more preferably
10.sup.4 to 10.sup.6 particles per dose.
[0106] In another preferred aspect, the immunogenic composition of
the invention comprises an amount of the PRRS virus according to
the invention which is equivalent to a virus titre of at least
about 10.sup.3 TCID.sub.50/mL per dose, preferably between 10.sup.3
to 10.sup.5 TCID.sub.50/mL per dose
[0107] In yet another preferred aspect, the immunogenic composition
of the invention further contains one or more pharmaceutically
acceptable carriers or excipients. Said one or more
pharmaceutically acceptable carriers or excipients are preferably
selected from the group consisting of solvents, dispersion media,
adjuvants, stabilizing agents, diluents, preservatives,
antibacterial and antifungal agents, isotonic agents, and
adsorption delaying agents.
[0108] The present invention is further directed to the immunogenic
composition of the invention for use in a method for [0109]
inducing an immune response against PRRSV, or [0110] the prevention
or reduction of PRRS, or [0111] the prevention or reduction of
PRRSV viremia and/or [0112] preventing or reducing clinical
symptoms, in particular the elevated body temperature, associated
with PRRSV infection, or [0113] the prevention or reduction of the
elevated body temperature associated with the administration of an
attenuated PRRSV vaccine to an animal.
[0114] As used herein, the term "inducing an immune response" to an
antigen or composition is the development of a humoral and/or
cellular immune response in an animal to an antigen present in the
composition of interest.
[0115] The term "prevention" or "reduction" or "preventing" or
"reducing", respectively, as used herein, means, but is not limited
to a process which includes the administration of a PRRSV antigen,
namely of the PRRSV according to the invention which is included in
the composition of the invention, to an animal, wherein said PRRSV
antigen, when administered to said animal elicits or is able to
elicit an immune response in said animal against PRRSV. Altogether,
such treatment results in reduction of the clinical symptoms of
PRRS or of symptoms associated with PRRSV infection, respectively.
More specifically, the term "prevention" or "preventing, as used
herein, means generally a process of prophylaxis in which an animal
is exposed to the immunogenic composition of the present invention
prior to the induction or onset of the disease process (PRRS).
[0116] Herein, "reduction of PRRS" or "reduction of clinical
symptoms associated with PRRSV infection" means, but is not limited
to, reducing the number of infected subjects in a group, reducing
or eliminating the number of subjects exhibiting clinical symptoms
of infection, or reducing the severity of any clinical symptoms
that are present in the subjects, in comparison to wild-type
infection. For example, it should refer to any reduction of
pathogen load, pathogen shedding, reduction in pathogen
transmission, or reduction of any clinical sign symptomatic of
PRRSV infection, in particular of elevated body temperature.
Preferably these clinical symptoms are reduced in subjects
receiving the composition of the present invention by at least 10%
in comparison to subjects not receiving the composition and may
become infected. More preferably, clinical symptoms are reduced in
subjects receiving the composition of the present invention by at
least 20%, preferably by at least 30%, more preferably by at least
40%, and even more preferably by at least 50%.
[0117] Also, the elevated body temperature usually associated with
the administration of an attenuated PRRSV vaccine to an animal is
reduced in subjects receiving the composition of the present
invention by at least 10% in comparison to subjects receiving a
conventional attenuated PRRSV vaccine. More preferably, the
elevated body temperature usually associated with the
administration of an attenuated PRRSV vaccine is reduced in
subjects receiving the composition of the present invention by at
least 20%, preferably by at least 30%, more preferably by at least
40%, and even more preferably by at least 50%.
[0118] Hence, a further advantage of the PRRS virus according to
the invention is in particular the effect that its administration
to an animal, e.g. for the prophylaxis or treatment of PRRS,
results in a reduced increase of body temperature in the animal in
comparison with a conventional attenuated PPRSV vaccine.
[0119] The term "conventional attenuated PRRSV vaccine", as
mentioned herein, is in particular directed to any attenuated PRRSV
useful as a vaccine, wherein said PRRSV does not have the
characteristic features of the PRRS virus of the present invention,
in particular is not able to induce the interferon type I
production and secretion by a cell infected by said PRRSV.
[0120] The term "subject", as mentioned herein, in particular
relates to an animal.
[0121] The term "body temperature", as used herein, in particular
refers to the approximate average normal, internal temperature of
an animal, for example about 38.5-39.degree. C. in pigs, whereas
the body temperature associated with a PRRSV infection may be
elevated up to 41.degree. C. in pigs.
[0122] The term "reduction of PRRSV viremia" means, but is not
limited to, the reduction of PRRS virus entering the bloodstream of
an animal, wherein the viremia level, i.e. the number of PRRSV RNA
copies per mL of blood serum or the number of plaque forming
colonies per deciliter of blood serum, is reduced in the blood
serum of subjects receiving the composition of the present
invention by at least 50% in comparison to subjects not receiving
the composition and may become infected. More preferably the
viremia level is reduced in subjects receiving the composition of
the present invention by at least 90%, preferably by at least
99.9%, more preferably by at least 99.99%, and even more preferably
by at least 99.999%.
[0123] The present invention further relates to the use of the PRRS
virus according to the invention or of the immunogenic composition
of the invention for the preparation of a vaccine or medicament for
[0124] inducing an immune response against PRRSV, or [0125]
treating or preventing PRRS, or [0126] preventing or reducing PRRSV
viremia and/or [0127] preventing or reducing clinical symptoms, in
particular the elevated body temperature, associated with PRRSV
infection, or [0128] the prevention or reduction of the elevated
body temperature associated with the administration of an
attenuated PRRSV vaccine to an animal.
[0129] In particular, it is preferred if the vaccine or medicament,
as mentioned herein, is to be administered in two doses or
preferably in a single dose to an animal.
[0130] The present invention also provides a method of preparing an
immunogenic composition, preferably a vaccine or medicament, more
preferably a vaccine or medicament for [0131] inducing an immune
response against PRRSV, or [0132] treating or preventing PRRS, or
[0133] preventing or reducing PRRSV viremia and/or [0134]
preventing or reducing clinical symptoms, in particular the
elevated body temperature, associated with PRRSV infection, or
[0135] the prevention or reduction of the elevated body temperature
associated with the administration of an attenuated PRRSV vaccine
to an animal, wherein said method comprises the step of mixing the
PRRS virus according to the invention with one or more
pharmaceutically acceptable carriers or excipients, and wherein
said one or more pharmaceutically acceptable carriers or excipients
are preferably selected from the group consisting of solvents,
dispersion media, adjuvants, stabilizing agents, diluents,
preservatives, antibacterial and antifungal agents, isotonic
agents, and adsorption delaying agents.
[0136] In another aspect, the present invention further provides a
method for [0137] inducing an immune response against PRRSV, or
[0138] treating or preventing PRRS, or [0139] preventing or
reducing PRRSV viremia and/or [0140] preventing or reducing
clinical symptoms, in particular the elevated body temperature,
associated with PRRSV infection, or [0141] the prevention or
reduction of the elevated body temperature associated with the
administration of an attenuated PRRSV vaccine to an animal, wherein
said method comprises the step of administering the immunogenic
composition of the invention to an animal in need thereof, and
wherein preferably the immunogenic composition and/or the vaccine
is administered in two doses or, more preferably, in a single
dose.
Examples
[0142] In the examples five viable, genetically designed PRRSV
mutant strains are described which are based on infectious EU PRRSV
cDNA clone LoN94-13. These strains, delta nsp1 IX-10, delta nsp1
XVII-1, delta nsp1 XVIII-12, delta nsp1 XIX-2 and delta nsp1 XX-9
(henceforth referred to as vaccine candidates), harbor genomic
deletions of two, three, four, five, or six codons in their
predicted nsp1 genes, respectively, resulting in deletions of two
(motif P21R22), three (motif R20P21R22), four (motif G19R20P21R22),
five (motif N18G19R20P21R22), or six (motif (P17N18G19R20P21R22)
amino acids in their predicted nsp1.beta. proteins, respectively
(FIG. 1).
[0143] Based on sequence alignments of parental strain LoN94-13
with PRRSV US and EU reference strains VR-2332 and Lelystad virus
as well as with strain GD-XH, the deletions are located in the
predicted nsp1.beta. portion of nsp1 (FIG. 2). In more detail, the
deletion site for all vaccine candidates is located in the
N-terminal domain (NTD) of nsp1.beta. and overlaps with amino acids
P23R24 of GD-XH nsp1.beta.; (FIG. 2).
[0144] After transfection of synthetic transcripts of the vaccine
candidates into BHK21 cells and transfer of cell culture
supernatant from transfected BHK21 cells onto PRRSV-susceptible
MA104 cells, plaque formation typical for PRRSV infection occurred.
PRRSV-specificity and viability for each of the vaccine strains
then was demonstrated by subsequent cell culture passages on MA104
cells and PRRSV-specific immunofluorescence using monoclonal
antibody SDOW17 (Rural Technologies).
[0145] After endpoint dilution and generation of virus stocks each
derived from material of a single virus plaque, virus titers of the
obtained virus stocks were determined for each vaccine candidate by
serial virus titrations on 96-well plates containing MA104 cells
followed by PRRSV-specific immunofluorescence analyses six to seven
days post infection. Unlike experience with titrations of virus
stocks from parental PRRSV LoN94-13, the first serial dilutions of
vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 did
not demonstrate a cytopathic effect and virus plaque formation,
while at higher dilutions of the virus stocks a cytopathic effect
was detectable. Moreover, when respective titrations were
investigated by immunofluorescence, cell culture wells of the first
serial dilutions were negative for PRRSV infection for both vaccine
candidates, while wells infected with higher dilutions of the virus
stocks showed PRRSV-specific immunofluorescence, respectively (FIG.
3A and FIG. 3B).
[0146] To determine whether the prepared vaccine candidate virus
stocks contained type I IFN, a commercial ELISA specific for human
IFN.beta. (Invitrogen) was used. MA104 cells are epithelial Green
Monkey kidney cells. According to the ELISA manufacturer, this
Invitrogen ELISA is also suited for the detection of primate
IFN.beta. other than human. For each vaccine candidate's virus
stock, 100 .mu.l served as assay input, while a virus stock from
parental strain LoN94-13, cell culture medium, and medium from
noninfected cells served as controls. For quantification of the
obtained results, a calibration curve was included using a positive
control of the ELISA manufacturer. All samples were measured in
duplicates. Unlike the negative controls, virus stocks of the
vaccine candidates contained considerable levels of type I IFN,
while the virus stock of the parental virus showed IFN levels as
low as the negative controls (FIG. 4).
[0147] To confirm the results obtained and to assess kinetics of
type I IFN production in cells infected with the vaccine
candidates, a time course experiment was performed using MA104
cells infected at a multiplicity of infection (MOI) of 0.001,
respectively. Parental strain LoN94-13 served as negative control.
While there were only very little and unaltered levels of type I
IFN near background detectable for infection with parental strain
LoN94-13, vaccine candidates delta nsp1 XVII-1 and delta nsp1
XVIII-12 induced considerable and increasing amounts of up to about
18 I.U. IFN.beta. per 25 .mu.l sample volume from two days post
infection on (FIG. 5).
[0148] It was experimentally assessed whether vaccine candidates
containing genomic deletions in nsp1 demonstrate an increased
sensitivity to type I IFN (FIG. 6). 5.times.10.sup.5 MA104 cells
were seeded into a well of a six-well plate and were either not
infected (n.inf.) or infected with 800 infectious virus particles
of one of the virus strains given on top, respectively. Cells then
were either inoculated with 120 I.U. human IFN.beta. (+IFN.beta.,
bottom row), respectively, or not (-IFN.beta., top row). Three days
post infection, immunofluorescence analysis specific for the PRRSV
capsid protein was performed using monoclonal antibody SDOW17
(Rural Technologies). The total numbers of foci of PRRSV-infected
cells per well are given below, respectively.
[0149] This experiment demonstrated that inoculation with type I
IFN reduced the number of PRRSV infection events in cells after
inoculation with a defined number of infectious virus particles,
reflecting reduced viral infectivity of PRRSV when IFN was added.
This reduction was 80-fold for wild type virus LoN94-13 (FIG. 6).
In addition, for infection with wild type virus, foci of infected
cells were smaller than in the well not inoculated with IFN (FIG.
6). However, for vaccine strains delta nsp1 XVII-1 and delta nsp1
XVIII-12, viral infectivity was reduced to zero when INF was added
(FIG. 6). Thus, these vaccine candidates not only induce production
of type I IFN in infected cells (FIG. 4 and FIG. 5), but also
demonstrate increased sensitivity to type I INF when compared to
wild type PRRSV (FIG. 6). This is reflected by their dramatically
reduced viral infectivity when IFN.beta. is present.
[0150] Interestingly, the cells infected for the time course
experiment summarized in FIG. 5 not only produced considerable
amounts of IFN.beta., but at the end of the experiment at six days
post infection, cells infected with vaccine candidates delta nsp1
XVII-1 and delta nsp1 XVIII-12 showed signs of recovery from
usually lytical PRRSV infection. While cells infected with parental
strain LoN94-13 were fully lysed, cells infected with the vaccine
candidates grew in a partially (delta nsp1 XVIII-12) or completely
intact monolayer (delta nsp1 XVII-1). For the latter, only weak
signs of a PRRSV-induced cytopathic effect were still detectable.
Thus, the interferon production of infected cells together with the
observed sensitivity of vaccine candidates to type I IFN correlated
with partial or almost complete recovery of infected cells over
time. It is reasonable to expect that type I IFN induction by the
vaccine candidates together with their increased sensitivity to
type I IFN will contribute to a significantly attenuated viral
phenotype in the natural host. In particular, expected features of
the vaccine candidates' attenuation in pigs include stimulation of
the innate and specific immunity, both humoral and cellular, and
less shedding and/or shortened viremia of the vaccine viruses.
[0151] To assess whether the PRRSV vaccine candidates are
attenuated in the host, an animal experiment in piglets was
performed.
[0152] Three groups, each of ten animals, were infected at study
day 0 either with wild-type parental EU PRRSV strain Lon94-13 (WT
group), or with delta nsp1 XVIII-12 (nsp1 group), or were not
infected (Ch control group). Infection was applied by intramuscular
injection to the neck at dosages of 10.sup.6,56 TCID.sub.50 for
LoN94-13 or 10.sup.6,6 TCID.sub.50 for delta nsp1 XVIII-12,
respectively. 21 days post vaccination, all animals were challenged
with a virulent EU PRRSV strain being heterologous to LoN94-13 by
intramuscular injection and intranasal inoculation at a total
dosage of 3.times.10.sup.6,52 TCID.sub.50. Animals were kept until
the end of the experiment at day 31, ten days after challenge, and
body temperatures were measured for all animals at days 0 (1 and 4
hours post vaccination), 1, 3, 5, 8, 10, 12, 14, 18, 20, 22, 24,
26, 28, and 31.
[0153] Mean body temperatures were determined for each animal for
the time after vaccination but before challenge using measured body
temperature data from all timepoints from day 0 through day 20.
Subsequently, mean body temperatures were determined for each group
(FIG. 7, (left-hand) columns). Error bars indicate standard
deviations, respectively. Following the same procedure, mean body
temperatures and standard deviations were determined for all groups
for the time after challenge using measured body temperature data
from all timepoints from day 22 through 31 (FIG. 7, (right-hand)
columns).
[0154] The term "Significant(ly)" in the context of the following
means either (i) p-values of 0.05 or lower as determined by the
Dunnett test and obtained from comparing the nsp1 group with either
the WT or the Ch control group for either of the two time periods
investigated (before and after challenge) or (ii) p-values of 0.05
or lower when comparing the mean temperature change within a group
and between the two time periods investigated (before and after
challenge).
[0155] When comparing the determined mean body temperatures for the
time after vaccination but before challenge (left hand columns) in
between the three groups, animals from the WT group demonstrated a
rise in body temperature of more than 0.4.degree. C. when compared
to animals from the noninfected Ch control group, thus
demonstrating virulence of LoN94-13 in the infected host. In
contrast, the nsp1 group showed a significant reduction in mean
body temperature of more than 0.2.degree. C. when compared to the
WT group. Thus, since vaccination dosages were the same for the WT
and the nsp1 group, the considerable reduction in increase of body
temperature compared to WT demonstrates that the described mutation
in the genome of delta nsp1 XVIII-12 has significantly reduced
virulence of the WT parental strain LoN94-13 in the infected
animal.
[0156] The significant rise in the mean body temperature of the Ch
control group from before challenge to after challenge of
0.4.degree. C. demonstrates virulence of the heterologous EU PRRSV
challenge strain. The mean temperature of the WT group after
challenge was slightly lower than before challenge, but not
significantly reduced (FIG. 7). In contrast, the mean body
temperature of the nsp1 group after challenge was significantly
reduced by almost 0.2.degree. C. when compared to that before
challenge. Moreover, the body temperature of the nsp1 group after
challenge was significantly reduced by almost 0.4.degree. C. when
compared to the Ch control group after challenge. Also, mean body
temperature of the nsp1 group after challenge was significantly
lower that that of the WT group after challenge by more than
0.2.degree. C. Taken together, this demonstrates that a measurable
and significant degree of protection from signs of disease induced
by the applied challenge virus was conferred to pigs by vaccination
with delta nsp1 XVIII-12. Since parental PRRSV strain LoN94-13 did
not confer significant protection, it is evident that the described
mutation in the genome of delta nsp1 XVIII-12 is causative for the
observed significant protective technical effect.
[0157] Analogous experiments, wherein the vaccination was performed
with lower amounts (10.sup.5 TCID.sub.50) of delta nsp1 XVIII-12
showed results similar to the above described results (data not
shown). Thus, in practice, a preferred amount of 10.sup.3 to
10.sup.5 TCID.sub.50 is sufficient for vaccination.
[0158] Taken together, the invention described here represents the
first known viable PRRSV (EU) strains that contain mutations
(deletions) in the nsp1 gene (nsp1.beta.) that induce type I IFN
(IFN.beta.) production in susceptible cells (MA104) and that show
increased sensitivity to type I IFN (IFN.beta.). Moreover, the
animal data demonstrates that (i) vaccine candidate delta nsp1
XVIII-12 is significantly attenuated in the host when compared to
its parental PRRSV strain LoN94-13 and that (ii) vaccine candidate
delta nsp1 XVIII-12 confers significant protection from signs of
disease induced by challenge with a heterologous PRRSV strain while
parental strain LoN94-13 does not. Thus, the described vaccine
candidates or the described mutations therein, either alone or
combined with other attenuating mutations, may serve as promising
life attenuated PRRSV vaccines.
In the Sequence Listing
[0159] SEQ ID NO: 1 corresponds to LoN94-13 complete nsp1
protein,
[0160] SEQ ID NO: 2 corresponds to Lelystad virus complete nsp1
protein,
[0161] SEQ ID NO: 3 corresponds to VR2332 complete nsp1
protein,
[0162] SEQ ID NO: 4 corresponds to LoN94-13 complete nsp1
Alpha,
[0163] SEQ ID NO: 5 corresponds to Lelystad virus complete nsp1
Alpha,
[0164] SEQ ID NO: 6 corresponds to VR2332 complete nsp1 Alpha,
[0165] SEQ ID NO: 7 corresponds to LoN94-13 complete nsp1 Beta,
[0166] SEQ ID NO: 8 corresponds to Lelystad virus complete nsp1
Beta,
[0167] SEQ ID NO: 9 corresponds to VR2332 complete nsp1 Beta,
[0168] SEQ ID NO: 10 corresponds to LoN94-13 nsp1Beta NTD,
[0169] SEQ ID NO: 11 corresponds to Lelystad virus nsp1 Beta
NTD,
[0170] SEQ ID NO: 12 corresponds to VR2332 nsp1 Beta NTD,
[0171] SEQ ID NO: 13 corresponds to LoN94-13 complete viral cDNA
insert,
[0172] SEQ ID NO: 14 corresponds to Lelystad virus complete
genome,
[0173] SEQ ID NO: 15 corresponds to VR2332 complete genome,
[0174] SEQ ID NO: 16 corresponds to delta nsp1 IX-10 complete nsp1
protein sequence,
[0175] SEQ ID NO: 17 corresponds to delta nsp1 XVII-1 complete nsp1
protein sequence,
[0176] SEQ ID NO: 18 corresponds to delta nsp1 XVIII-12 complete
nsp1 protein sequence,
[0177] SEQ ID NO: 19 corresponds to delta nsp1 XIX-2 complete nsp1
protein sequence,
[0178] SEQ ID NO: 20 corresponds to delta nsp1 XX-9 complete nsp1
protein sequence,
[0179] SEQ ID NO: 21 corresponds to delta nsp1 IX-10 complete
nsp1Beta protein sequence,
[0180] SEQ ID NO: 22 corresponds to delta nsp1 XVII-1 complete
nsp1Beta protein sequence,
[0181] SEQ ID NO: 23 corresponds to delta nsp1 XVIII-12 complete
nsp1 Beta protein sequence,
[0182] SEQ ID NO: 24 corresponds to delta nsp1 XIX-2 complete nsp1
Beta protein sequence,
[0183] SEQ ID NO: 25 corresponds to delta nsp1 XX-9 complete
nsp1Beta protein sequence,
[0184] SEQ ID NO: 26 corresponds to delta nsp1 IX-10 nsp1 Beta NTD
protein sequence,
[0185] SEQ ID NO: 27 corresponds to delta nsp1 XVII-1 nsp1Beta NTD
protein sequence,
[0186] SEQ ID NO: 28 corresponds to delta nsp1 XVIII-12 nsp1Beta
NTD protein sequence,
[0187] SEQ ID NO: 29 corresponds to delta nsp1 XIX-2 nsp1 Beta NTD
protein sequence,
[0188] SEQ ID NO: 30 corresponds to delta nsp1 XX-9 nsp1Beta NTD
protein sequence,
[0189] SEQ ID NO: 31 corresponds to delta nsp1 IX-10 complete viral
cDNA insert sequence,
[0190] SEQ ID NO: 32 corresponds to delta nsp1 XVII-1 complete
viral cDNA insert sequence,
[0191] SEQ ID NO: 33 corresponds to delta nsp1 XVIII-12 complete
viral cDNA insert sequence,
[0192] SEQ ID NO: 34 corresponds to delta nsp1 XIX-2 complete viral
cDNA insert sequence,
[0193] SEQ ID NO: 35 corresponds to delta nsp1 XX-9 complete viral
cDNA insert sequence,
[0194] SEQ ID NO: 36 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 2 aa),
[0195] SEQ ID NO: 37 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 3 aa),
[0196] SEQ ID NO: 38 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 4 aa),
[0197] SEQ ID NO: 39 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 5 aa),
[0198] SEQ ID NO: 40 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 6 aa),
[0199] SEQ ID NO: 41 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 7 aa),
[0200] SEQ ID NO: 42 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 2 aa),
[0201] SEQ ID NO: 43 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 3 aa),
[0202] SEQ ID NO: 44 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 4 aa),
[0203] SEQ ID NO: 45 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 5 aa),
[0204] SEQ ID NO: 46 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 6 aa),
[0205] SEQ ID NO: 47 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 7 aa),
[0206] SEQ ID NO: 48 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 2 aa),
[0207] SEQ ID NO: 49 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 3 aa),
[0208] SEQ ID NO: 50 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 4 aa),
[0209] SEQ ID NO: 51 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 5 aa),
[0210] SEQ ID NO: 52 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 6 aa),
[0211] SEQ ID NO: 53 corresponds to a (type 1) nsp1 Beta NTD with
mutation (deletion of 7 aa),
[0212] SEQ ID NO: 54 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 2 aa),
[0213] SEQ ID NO: 55 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 3 aa),
[0214] SEQ ID NO: 56 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 4 aa),
[0215] SEQ ID NO: 57 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 5 aa),
[0216] SEQ ID NO: 58 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 6 aa),
[0217] SEQ ID NO: 59 corresponds to a (type 2) nsp1 Beta NTD with
mutation (deletion of 7 aa).
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Sequence CWU 1
1
841385PRTPorcine reproductive and respiratory syndrome virus 1Met
Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5 10
15 Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala
Ile Gly 35 40 45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys
Val Pro Ile Gly 50 55 60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly
Cys Cys Trp Leu Ser Gly 65 70 75 80 Ile Phe Pro Leu Ala Arg Met Thr
Ser Gly Asn His Asn Phe Leu Gln 85 90 95 Arg Leu Val Lys Val Ala
Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110 Ser Arg His Leu
Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro
Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140
Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys
Pro Phe 165 170 175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe 180 185 190 Thr Asp Ser Ser Pro Asn Gly Arg Pro Arg
Met Met Trp Thr Pro Glu 195 200 205 Ser Asp Asp Ser Ala Asp Leu Glu
Ala Leu Pro Pro Glu Leu Glu Arg 210 215 220 Gln Val Glu Ile Leu Ile
Arg Ser Phe Pro Ala His His Pro Val Ser 225 230 235 240 Leu Ala Asp
Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe 245 250 255 Ser
Thr Tyr His Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe 260 265
270 Asp Ser Lys Cys Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val
275 280 285 Arg Leu Tyr Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln
Thr Lys 290 295 300 Trp Gly Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu
Gln Val Asn Gly 305 310 315 320 Ile Arg Ala Val Ile Asp Pro Asp Gly
Pro Ile His Val Glu Ala Leu 325 330 335 Ser Cys Pro Gln Ser Trp Ile
Arg His Leu Thr Leu Asp Asp Asp Val 340 345 350 Thr Pro Gly Phe Val
Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr 355 360 365 Glu Pro Thr
Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370 375 380 Gly
385 2385PRTPorcine reproductive and respiratory syndrome virus 2Met
Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5 10
15 Phe Trp Asn Ala Gly Gln Val Phe Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu Leu Ser Pro Glu Leu Gln Asp Thr Asp Leu Gly Ala
Val Gly 35 40 45 Leu Phe Tyr Lys Pro Arg Asp Lys Leu His Trp Lys
Val Pro Ile Gly 50 55 60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly
Cys Cys Trp Leu Ser Ala 65 70 75 80 Val Phe Pro Leu Ala Arg Met Thr
Ser Gly Asn His Asn Phe Leu Gln 85 90 95 Arg Leu Val Lys Val Ala
Asp Val Leu Tyr Arg Asp Gly Cys Leu Ala 100 105 110 Pro Arg His Leu
Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Asn Trp 115 120 125 Tyr Pro
Ile Thr Gly Pro Val Pro Gly Met Gly Leu Phe Ala Asn Ser 130 135 140
Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys
Pro Phe 165 170 175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe 180 185 190 Thr Asp Ser Ser Leu Asn Gly Arg Ser Arg
Met Met Trp Thr Pro Glu 195 200 205 Ser Asp Asp Ser Ala Ala Leu Glu
Val Leu Pro Pro Glu Leu Glu Arg 210 215 220 Gln Val Glu Ile Leu Ile
Arg Ser Phe Pro Ala His His Pro Val Asp 225 230 235 240 Leu Ala Asp
Trp Glu Leu Thr Glu Ser Pro Glu Asn Gly Phe Ser Phe 245 250 255 Asn
Thr Ser His Ser Cys Gly His Leu Val Gln Asn Pro Asp Val Phe 260 265
270 Asp Gly Lys Cys Trp Leu Ser Cys Phe Leu Gly Gln Ser Val Glu Val
275 280 285 Arg Cys His Glu Glu His Leu Ala Asp Ala Phe Gly Tyr Gln
Thr Lys 290 295 300 Trp Gly Val His Gly Lys Tyr Leu Gln Arg Arg Leu
Gln Val Arg Gly 305 310 315 320 Ile Arg Ala Val Val Asp Pro Asp Gly
Pro Ile His Val Glu Ala Leu 325 330 335 Ser Cys Pro Gln Ser Trp Ile
Arg His Leu Thr Leu Asp Asp Asp Val 340 345 350 Thr Pro Gly Phe Val
Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr 355 360 365 Glu Pro Thr
Thr Ser Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370 375 380 Gly
385 3383PRTPorcine reproductive and respiratory syndrome virus 3Met
Ser Gly Ile Leu Asp Arg Cys Thr Cys Thr Pro Asn Ala Arg Val 1 5 10
15 Phe Met Ala Glu Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu Leu Pro Leu Asn Leu Gln Val Ser Glu Leu Gly Val
Leu Gly 35 40 45 Leu Phe Tyr Arg Pro Glu Glu Pro Leu Arg Trp Thr
Leu Pro Arg Ala 50 55 60 Phe Pro Thr Val Glu Cys Ser Pro Ala Gly
Ala Cys Trp Leu Ser Ala 65 70 75 80 Ile Phe Pro Ile Ala Arg Met Thr
Ser Gly Asn Leu Asn Phe Gln Gln 85 90 95 Arg Met Ile Arg Val Ala
Ala Glu Leu Tyr Arg Ala Gly Gln Leu Thr 100 105 110 Pro Ala Val Leu
Lys Ala Leu Gln Val Tyr Glu Arg Gly Cys Arg Trp 115 120 125 Tyr Pro
Ile Val Gly Pro Ala Pro Gly Val Ala Val Tyr Ala Asn Ser 130 135 140
Leu His Val Ser Asp Lys Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Leu Pro Leu Pro Gln Arg Pro Lys Pro Glu Asp Phe Cys
Pro Phe 165 170 175 Glu Cys Ala Met Ala Thr Val Tyr Asp Ile Gly His
Asp Ala Val Met 180 185 190 Tyr Val Ala Glu Arg Lys Ile Ser Trp Ala
Pro Arg Gly Gly Asp Glu 195 200 205 Val Lys Phe Glu Ala Val Pro Gly
Glu Leu Arg Leu Ile Ala Asn Arg 210 215 220 Leu Arg Thr Ser Phe Pro
Pro His His Thr Val Asp Met Ser Lys Phe 225 230 235 240 Ala Phe Thr
Ala Pro Gly Cys Gly Val Ser Met Arg Val Glu Arg Gln 245 250 255 His
Gly Cys Leu Pro Ala Asp Thr Val Pro Glu Gly Asn Cys Trp Trp 260 265
270 Ser Leu Phe Asp Ser Leu Pro Leu Glu Val Gln Asn Lys Glu Ile Arg
275 280 285 His Ala Asn Gln Phe Gly Tyr Gln Thr Lys His Gly Val Ser
Gly Lys 290 295 300 Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Leu Arg
Ala Val Thr Asp 305 310 315 320 Leu Asn Gly Pro Ile Val Val Gln Tyr
Phe Ser Val Lys Glu Ser Trp 325 330 335 Ile Arg His Leu Lys Leu Ala
Gly Glu Pro Ser Tyr Ser Gly Phe Glu 340 345 350 Asp Leu Leu Arg Ile
Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Asp 355 360 365 Lys Glu Glu
Lys Ile Phe Arg Phe Gly Ser His Lys Trp Tyr Gly 370 375 380
4180PRTPorcine reproductive and respiratory syndrome virus 4Met Ser
Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5 10 15
Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20
25 30 Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile
Gly 35 40 45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val
Pro Ile Gly 50 55 60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys
Cys Trp Leu Ser Gly 65 70 75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser
Gly Asn His Asn Phe Leu Gln 85 90 95 Arg Leu Val Lys Val Ala Asp
Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110 Ser Arg His Leu Arg
Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile
Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met
His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150
155 160 Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro
Phe 165 170 175 Glu Glu Ala His 180 5180PRTPorcine reproductive and
respiratory syndrome virus 5Met Ser Gly Thr Phe Ser Arg Cys Met Cys
Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn Ala Gly Gln Val Phe
Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser Leu Leu Ser Pro Glu
Leu Gln Asp Thr Asp Leu Gly Ala Val Gly 35 40 45 Leu Phe Tyr Lys
Pro Arg Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60 Ile Pro
Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Ala 65 70 75 80
Val Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85
90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu
Ala 100 105 110 Pro Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly
Cys Asn Trp 115 120 125 Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly
Leu Phe Ala Asn Ser 130 135 140 Met His Val Ser Asp Gln Pro Phe Pro
Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn Ser Pro Leu Pro Gln
Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175 Glu Glu Ala His
180 6180PRTPorcine reproductive and respiratory syndrome virus 6Met
Ser Gly Ile Leu Asp Arg Cys Thr Cys Thr Pro Asn Ala Arg Val 1 5 10
15 Phe Met Ala Glu Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg
20 25 30 Ser Leu Leu Pro Leu Asn Leu Gln Val Ser Glu Leu Gly Val
Leu Gly 35 40 45 Leu Phe Tyr Arg Pro Glu Glu Pro Leu Arg Trp Thr
Leu Pro Arg Ala 50 55 60 Phe Pro Thr Val Glu Cys Ser Pro Ala Gly
Ala Cys Trp Leu Ser Ala 65 70 75 80 Ile Phe Pro Ile Ala Arg Met Thr
Ser Gly Asn Leu Asn Phe Gln Gln 85 90 95 Arg Met Val Arg Val Ala
Ala Glu Leu Tyr Arg Ala Gly Gln Leu Thr 100 105 110 Pro Ala Val Leu
Lys Ala Leu Gln Val Tyr Glu Arg Gly Cys Arg Trp 115 120 125 Tyr Pro
Ile Val Gly Pro Val Pro Gly Val Ala Val Phe Ala Asn Ser 130 135 140
Leu His Val Ser Asp Lys Pro Phe Pro Gly Ala Thr His Val Leu Thr 145
150 155 160 Asn Leu Pro Leu Pro Gln Arg Pro Lys Pro Glu Asp Phe Cys
Pro Phe 165 170 175 Glu Cys Ala Met 180 7 205PRTPorcine
reproductive and respiratory syndrome virus 7Ser Ser Val Tyr Arg
Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Pro Asn Gly
Arg Pro Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser 20 25 30 Ala
Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 35 40
45 Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp
50 55 60 Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr
Tyr His 65 70 75 80 Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe
Asp Ser Lys Cys 85 90 95 Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile
Glu Val Arg Leu Tyr Glu 100 105 110 Asp Tyr Leu Ala Asn Ala Phe Gly
Tyr Gln Thr Lys Trp Gly Val Ser 115 120 125 Gly Lys Tyr Leu Gln Arg
Arg Leu Gln Val Asn Gly Ile Arg Ala Val 130 135 140 Ile Asp Pro Asp
Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln 145 150 155 160 Ser
Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe 165 170
175 Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr
180 185 190 Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195
200 205 8 205PRTPorcine reproductive and respiratory syndrome virus
8Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1
5 10 15 Leu Asn Gly Arg Ser Arg Met Met Trp Thr Pro Glu Ser Asp Asp
Ser 20 25 30 Ala Ala Leu Glu Val Leu Pro Pro Glu Leu Glu Arg Gln
Val Glu Ile 35 40 45 Leu Ile Arg Ser Phe Pro Ala His His Pro Val
Asp Leu Ala Asp Trp 50 55 60 Glu Leu Thr Glu Ser Pro Glu Asn Gly
Phe Ser Phe Asn Thr Ser His 65 70 75 80 Ser Cys Gly His Leu Val Gln
Asn Pro Asp Val Phe Asp Gly Lys Cys 85 90 95 Trp Leu Ser Cys Phe
Leu Gly Gln Ser Val Glu Val Arg Cys His Glu 100 105 110 Glu His Leu
Ala Asp Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val His 115 120 125 Gly
Lys Tyr Leu Gln Arg Arg Leu Gln Val Arg Gly Ile Arg Ala Val 130 135
140 Val Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln
145 150 155 160 Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr
Pro Gly Phe 165 170 175 Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn
Thr Glu Pro Thr Thr 180 185 190 Ser Arg Ile Phe Arg Phe Gly Ala His
Lys Trp Tyr Gly 195 200 205 9 203PRTPorcine reproductive and
respiratory syndrome virus 9Ala Thr Val Tyr Asp Ile Gly His Asp Ala
Val Met Tyr Val Ala Glu 1 5 10 15 Arg Lys Ile Ser Trp Ala Pro Arg
Gly Gly Asp Glu Val Lys Phe Glu 20 25 30 Ala Val Pro Gly Glu Leu
Arg Leu Ile Ala Asn Arg Leu Arg Thr Ser 35 40 45 Phe Pro Pro His
His Thr Val Asp Met Ser Lys Phe Ala Phe Thr Ala 50 55 60 Pro Gly
Cys Gly Val Ser Met Arg Val Glu Arg Gln His Gly Cys Leu 65 70 75 80
Pro Ala Asp Thr Val Pro Glu Gly Asn Cys Trp Trp Ser Leu Phe Asp 85
90 95 Ser Leu Pro Leu Glu Val Gln Asn Lys Glu Ile Arg His Ala Asn
Gln 100 105 110 Phe Gly Tyr Gln Thr Lys His Gly Val Ser Gly Lys Tyr
Leu Gln Arg 115 120
125 Arg Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp Leu Asn Gly Pro
130 135 140 Ile Val Val Gln Tyr Phe Ser Val Lys Glu Ser Trp Ile Arg
His Leu 145 150 155 160 Lys Leu Ala Gly Glu Pro Ser Tyr Ser Gly Phe
Glu Asp Leu Leu Arg 165 170 175 Ile Arg Val Glu Pro Asn Thr Ser Pro
Leu Ala Asp Lys Glu Glu Lys 180 185 190 Ile Phe Arg Phe Gly Ser His
Lys Trp Tyr Gly 195 200 1052PRTPorcine reproductive and respiratory
syndrome virus 10Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
Thr Asp Ser Ser 1 5 10 15 Pro Asn Gly Arg Pro Arg Met Met Trp Thr
Pro Glu Ser Asp Asp Ser 20 25 30 Ala Asp Leu Glu Ala Leu Pro Pro
Glu Leu Glu Arg Gln Val Glu Ile 35 40 45 Leu Ile Arg Ser 50
1152PRTPorcine reproductive and respiratory syndrome virus 11Ser
Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Leu Asn Gly Arg Ser Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser
20 25 30 Ala Ala Leu Glu Val Leu Pro Pro Glu Leu Glu Arg Gln Val
Glu Ile 35 40 45 Leu Ile Arg Ser 50 1248PRTPorcine reproductive and
respiratory syndrome virus 12Ala Thr Val Tyr Asp Ile Gly His Asp
Ala Val Met Tyr Val Ala Glu 1 5 10 15 Arg Lys Ile Ser Trp Ala Pro
Arg Gly Gly Asp Glu Val Lys Phe Glu 20 25 30 Ala Val Pro Gly Glu
Leu Arg Leu Ile Ala Asn Arg Leu Arg Thr Ser 35 40 45 13
15116DNAPorcine reproductive and respiratory syndrome virus
13atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag
60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct
120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct
cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat
tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct
cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt
ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc
420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc
ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc
gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg
ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca
gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac
720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg
tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacggtcg
gcctcgcatg atgtggacgc 840cggaatccga tgattcagcc gacctagagg
cgctaccgcc tgagctagaa cgtcaggtcg 900aaatcctcat tcggagtttt
cctgcccatc accctgtcag cctggccgac tgggagctcg 960ctgagtcccc
tgagaacggt ttttccttca gcacgtacca ttctggtggt tatcttgtcc
1020aaaaccccga cgtgtttgac agcaagtgct ggctctcctg tttcttggat
cagccgatcg 1080aagtgcgcct ctatgaggat tatctggcta acgctttcgg
ttaccaaacc aagtggggcg 1140tgtctggtaa gtacctccag cgcaggcttc
aagtcaacgg tattcgtgct gtaatcgatc 1200ctgatggccc cattcacgtt
gaagcgctgt cttgccccca atcttggatc aggcacctga 1260ctctggacga
tgacgtcacc ccaggattcg ttcgcctaac atccctccgc attgtgccga
1320acacagagcc cactactctc cgaatctttc ggtttggagc gcataagtgg
tatggcgctg 1380ccggcaaacg ggctcgtgcc aagcgtgccg ctaaaagtga
gaagggtccg gctcccactc 1440ccaaggttgc cccgccagcc cccacctgcg
gaattgttac ctactctcca ccaacagacg 1500ggtcttgcgg ttggcacgtc
cttgccgcca taatgaaccg aatgatgaac ggtgacttca 1560tgtcccctct
ggcccagtac aacagaccag aggatgattg ggcttctgat tacgatcttg
1620cccaggcgat tcaatgtctg cggttgcctg ctaccatagt tcggaatcgt
gcctgtccca 1680acgccaagta cctcataaaa ctcaacggag tccactggga
ggtagaggtg aggtcaggga 1740tggcccctcg ctcccttccc cgcgagtgcg
tagtcggcgt ttgttccgaa ggctgtgccg 1800catcgcctta cccagaaaac
gggctaccta aacgagcgtt tgaggccttg gcgtctgctt 1860acagactacc
ttccgattgt gtcagttctg gtattgctga ctttcttgct aacccccctc
1920aggaattctg gactcttgac aaaatgttga cctccccgtc accggagcgg
tctggttttt 1980ccagtctgta caaattgcta ttagaggttg tcccgcagaa
atgcggagcc acggaagggg 2040ctttcaccta tgctgttgag agaatgctga
aggattgccc gagctccgaa caggccatgg 2100cccttctggc aaaaattaaa
gttccatcct caaaggcccc gtctgtgtcc ctggacgagt 2160gtttccctac
ggatgtttca gccgatttcg aaccagcatc tcaggagagg tcccaaaatt
2220ccagcgctgc tgttgtcctg cattcaccga atgcaaaaga gttcgaggaa
gcagctccag 2280gggaagttca ggagggtggc cacaaggccg tccactctgc
actccctgcc gggggtccta 2340acaataagca ggcacagctg gttgccggtg
agcaactgaa gctcggcggt tgtggttcgg 2400tagttgggaa tgcacatgaa
ggtgttctgg tcccacctgg tccaattaat ttgacaagcg 2460gggatttacc
ctcctcaggc tccatgaaag aagatatgct caatagccgg gaggacgaac
2520cactggattt gtcccaacca gcaacagctg tcacaacgac tcttatggga
gagctaacac 2580ccgactacct aggttctgat actggtgccc tccccgtcac
cgtccgaaaa tttgtcccga 2640cggggcctat actccgtcat gttgagcact
gcagcacggg gtcgggcgat agcagttcgc 2700ctttggatct gtctggtgcg
caaaccccgg accagccttt aaatctgtcc ctggcggctt 2760ggccagtgag
gaccaccgcg tctgatcctg gctgggtcca cggtagacgc gagcctgtct
2820ttgtaaagcc tcgagatgtt ttctctgatg gcgattcagc ccttcagttc
ggggagcttt 2880ctgaatccag ctctgtcatc gagtttgatc gggcaaaaga
tgctcaggtg gctgacgccc 2940ctgtcggtct gacgacttcg gacgaggccc
tctccgcagt cgatcctttc gagttttccg 3000aactcaagcg cccacgtttc
tccgcacaag ccttaattga ccgaggcggc ccacttgccg 3060atgtccatgc
aaaaataaag aaccgggtat atgaacagtg cctccaagct tgtgagcccg
3120gtagtcgcgc aaccccagct accagggagt ggctcgacaa aatgtgggag
agagtggaca 3180tgaaaacctg gcgctgcacc tcacagttcc aagctggtca
cattcttgcg tccctcaaat 3240tcctccctga catgattcaa gacacaccgc
ctcctgttcc caggaagaac cgagccagtg 3300ataaagctgg cctgaaacaa
ctagtggcac agtgggatag gagattgagt tcaacccccc 3360ccccaaaacc
ggttgggccg gtacttgacc gggtcgatcc tccgcctacg ggtacccggc
3420aagaagacgt caccccctcc gatgggccac cccatgcgcc ggatggtcga
gtgagtacgg 3480gtgggagttg gaaaggcctt atgctttccg gcacccgtct
cacggggtcc atcagtcatc 3540gcctcatgac atgggttttt gaagttgtct
cccatctccc agcttttatg ctcacacttt 3600tctcgccgcg gggctctatg
gctccaggcg attgggtttt tgcaggtgtt gttttacttg 3660ctctcctgct
ctgtcgttct tacccaatat tcgggtgcct tcccttattg ggtgtctttt
3720ctggtactgt gcggcgtgtt cgtctgggtg tttttggctc ttggatggct
tttgctgtat 3780ttttattctc gactccatcc aacccagtcg gttcttcttg
taaccacgat tcgccggagt 3840gtcatgctga gcttctggct cttgagcagc
gccaactttg ggaacctgtg cgcggccttg 3900tggtcggccc atcgggcctc
ttatgtgtca ttcttggcaa gttactcggt gggtcacgtt 3960atctctggca
tgttctccta cgtctatgcc tgcttgcaga tttggccctt tctcttgttt
4020atgtggtgtc ccaggggcgt tgtcacaagt gttggggaaa gtgtataagg
acagctcctg 4080ctgaggtggc ttttaatgta tttcctttct cgcgcgccac
ccgtagttct cttgtatcct 4140tatgtgatcg attccaaacg ccaaaaggag
ttgatcccgt gcacttggcg acaggttggc 4200gcgggtgctg gcgtggtgag
agtcctatcc atcaaccaca ccaaaagccc atagcttacg 4260ccaatttgga
tgaaaagaag atatctgccc aaacggttgt tgctgtccca tacgatccca
4320atcaggctat caaatgtctg aaagttctgc aggcgggagg ggctatcgtg
gatcagccta 4380cgcctgaggt cgttcgtgtg tccgagatcc ccttctcagc
cccatttttc ccaaaagttc 4440cagtcaaccc agattgtagg gtcgtggtgg
attcggacac ttttgtggct gcggttcgtt 4500gtggttattc gacatcacaa
ctggtcctgg gccagggcaa ctttgccaag ttaaatcaaa 4560ccccccccag
gaactctatc tccaccaaag cgactggtgg ggcctcttat acttttgctg
4620tggctcaagt gtctgtgtgg acccttgttc atttcgtcct cggtctttgg
ctcacgtcac 4680ctcaagtgtg tggtcgagga accgctgacc catggtgttc
aagtccattt tcatatccta 4740cctatggccc cggggttgtg tgctcctctc
gactttgtgt gtctgctgac ggggtcaccc 4800ttccattgtt ctcagccgtg
gcacaactct ctggtaggga ggtggggatt tttattttag 4860tgctcgtctc
ctttattgcc ttggcccacc gcatggctct taaggcagac atgttagtag
4920tctttttggc tctttgtgct tatgcctggc ccatgagctc ctggttgatc
tgcttctttc 4980ctatactctt gaggtgggtt acccttcacc ctctcactat
gctttgggtg cattcattct 5040tgatgttttg tctcccagca gccggcgtcc
tctcactggg gataactggc ctcctctggg 5100caatcggccg ctttactcag
gttgccggaa tcattacacc ttatgatatc caccagtaca 5160cctctgggcc
acgtggtgca gccgctgtgg ccacagcccc agaaggcact tacatggccg
5220ccgtccggag agctgcttta accgggcgga ctttaatctt caccccgtcc
gcagttggat 5280cccttctcga aggtgctttc aggactcata aaccctgcct
taacaccgtg aatgtcgtag 5340gctcctccct tggttccgga ggggttttca
ccattgacgg aaaaaaaatt gtcgtcactg 5400ctgcccatgt gctgaacggc
gacacagcta gagtcaccgg tgattcctac aaccgcatgc 5460acactttcaa
gactaatggt gactatgcct ggtcccatgc tgataactgg cagggcgctg
5520cccctgtggt caaggttgcg aaagggtatc gcggtcgtgc ctactggcaa
acatcaactg 5580gtgtcgagcc tggtgttatt gggaatgggt tcgccttctg
tttcaccaac tgcggcgatt 5640cggggtcacc cgttatctca gaatctggtg
atcttatcgg aatccacacc ggttcaaaca 5700aacttggttc tggtcttgtg
acaacccctg aaggggagac ctgtaccatc agagaaacca 5760agctttctga
cctttccaga catttcgcag gcccaagcgt tcctcttggg gacatcaaat
5820tgagtccggc catcatccct gatgtgacat ccattccgag tgacttggca
tcgctcctag 5880cttccgtccc tgtaatggaa ggcggcctct cgaccgttca
acttttgtgt gtctttttcc 5940tcctctggcg catgatgggc catgcctgga
cgcccattgt tgccgtgggc ttctttttgc 6000tgaatgaaat tcttccagca
gttttggttc gagccgtgtt ttcttttgca ctctttgtgc 6060ttgcatgggc
caccccctgg tctgcacaag tgttgatgat tagacttctc acggcatctc
6120tcaaccgcaa caaactttct cttgcgttct acgcactcgg aggtgttgtt
ggtttggctg 6180ctgaaatcgg gacttttgct ggtaaattgt ctgaattgtc
tcaagctctt tcgacatact 6240gtttcttacc tagggtcctt gctatgacca
gctgtgttcc catcatcatc attggtggac 6300tccatgccct cggtgtgatt
ctgtggttat tcaaataccg gtgcctccac aacatgctgg 6360ttggtgatgg
aagcttttca agcgctttct tcctacggta ttttgcagag ggcaatctca
6420ggagaggtgt ttcacagtcc tgtggcatga gtaacgagtc cctgacggct
gctttggctt 6480gcaagttgtc acaggctgac cttgattttt tgtccagctt
aacgaacttc aagtgctttg 6540tatctgcttc aaacatgaaa aatgctgccg
gccagtacat tgaagcagct tatgccaggg 6600ccctgcgtca agagttggcc
tctttagtcc agattgacaa aatgaaagga gttttgtcca 6660agctagaggc
ctttgctgaa acggccactc cgtccctcga cgtaggtgac gtgattgttc
6720tacttggaca acatcctcac ggatccgttc tcgatattaa tgtggggact
gaaaggaaaa 6780ctgtatccgt gcaagagacc cggagcctag gcggctccaa
gttcagtgtt tgtactgttg 6840tgtcaaacac acccgtggac gccttagccg
gtattccact ccagacacca accccccttt 6900tcgagaatgg cccgcgtcat
cgcagcgagg aggacgatct taaagtcgag aggatgaaga 6960aacactgcgt
gtccctcggc ttccacaaca ttaacggtaa agtttactgc aagatttggg
7020acaagtctac cggtgacgcc ttttacactg atgattcccg gtacacccaa
gactatgctt 7080ttcaggacag gtcagctgac tatagagaca gggactacga
gggtgtgcaa accgcccccc 7140aacagggatt tgatccaaag tctgaaaccc
ctgttggtac cgttgtgatc ggcggtatta 7200cgtacaacag gtatttggtc
aaaggtaagg aggttctggt tcccaagcct gacaactgcc 7260ttgaagctgc
caagctgtcc cttgagcaag ctctcgctgg gatgggccaa acttgtgacc
7320ttacagctgc cgaggtggaa aagctaaagc gcatcattgg tcaacttcaa
ggattgacca 7380ctgagcaggc tttaaactgt tagccgccag cggcttgacc
cgctgtggcc gcggcggcct 7440agttgtaact gaaacggcgg taaaaattgt
caaataccac agcagaactt ttaccttagg 7500ctctttagac ctaaaagtca
cttccgaggt ggaggtgaag aagtcaaccg agcagggcca 7560cgctgttgtg
gcaaacttgt gttctggtgt cgtcttgatg agacctcacc caccgtccct
7620tgtcgacgtt cttctgaaac ccggacttga cataacaccc ggcattcaac
cagggcatgg 7680ggccgggaat atgggcgtgg acggttccat ttgggatttt
gaaaccgcac ccacaaaggc 7740tgaactcgag ttatccaagc aaataattca
agcatgtgaa gtcaggcgcg gggatgcccc 7800gaacctccag ctcccttaca
agctctatcc tgttagaggg gatcctgagc ggcataaagg 7860ccacctcatc
aataccaggt ttggagactt accttacaaa actcctcaag acaccaagtc
7920cgcaatccac gcggcttgtt gcctgcaccc caacggggcc cccgtgtctg
atggtaaatc 7980cacactaggt accactcttc aacatggctt cgagctttat
gtccctactg tgccctatag 8040tgtcatggag taccttgatt cacgcccaga
cacccctttt atgtgcacta aacacggcac 8100ttccaaggct gctgcagagg
acctccaaaa atacgaccta tccacccaag gatttgtcct 8160gcctggggtc
ctacgcctag tgcgcaggtt catctttggc cacattggca aggcaccgcc
8220attgttcctc ccatcaactt atcccgccaa gaactccatg gcaggtatta
atggtcagag 8280gttcccaaca aaggatgttc aaagtatacc tgaaattgat
gaaatgtgtg cccgcgccgt 8340caaggagaat tggcaaactg tgacaccttg
caccctcaag aaacagtatt gttctaggcc 8400caaaaccagg accatcctgg
gcactaacaa cttcatagcc ttggctcata gatcggcgct 8460cagtggtgtt
acccaggcat tcatgaagaa ggcttggaag tccccaatag ccttagggaa
8520aaacaaattc aaggagctgc attgcactgt cgctggcagg tgcctcgagg
ccgacttggc 8580ttcctgtgac cgcagcaccc ctgccattgt gaggtggttc
actacccacc tcctatatga 8640acttgcagga tgtgaagaat atctacctag
ctatgtgctt aactgttgcc atgaccttgt 8700ggcgacgcag gatggtgctt
tcacaaaacg cggtggcctg tcgtctggag acccagtcac 8760cagtgtgtcc
aacactgtgt actcactggt gatttatgcc cagcacatgg tactatctgc
8820cctgaaaatg ggtcatgaaa ttggcctcaa gttcctcgaa gaacaactca
aatttgagga 8880ccttcttgaa atccagccta tgttagtata ctctgatgat
cttgtcttgt acgcagaaaa 8940gcccaccttc cccaactatc attggtgggt
cgagcatctt gacctgatgt tgggctttaa 9000aacggaccca aagaaaaccg
tcataactga taaacccagt ttcctcgggt gcagaatcga 9060agcagggcga
cagctagtcc ccaatcgcga ccgcatcctg gctgctcttg catatcacat
9120gaaggcgcag aacgcctcag agtattatgc gtctgctgcc gcaatcctga
tggattcatg 9180tgcttgcatt gaccacgatc ctgaatggta tgaggacctc
atctgtggca ttgcccgatg 9240cgctcgcctg gacggttata gctttccagg
tccggcattt ttcatgtcca tgtgggagaa 9300gctgaggagt cataatgaag
ggaagaaatt ccgccactgc ggcatctgcg acgccaaagc 9360cgactacgcg
gctgcttgtg ggcttgattt gtgtttgttt cattcgcact ttcaccaaca
9420ctgccctgtc actctgagct gcggtcacca tgccggttcg aaggaatgtt
cgcagtgtca 9480gtcacctgtt ggggccggca gatcccctct tgatgctgtg
ctggaacaaa ttccatacaa 9540acctcctcgc actgtcatca tgaaggtggg
taataaaaca acggcccttg atccggggag 9600gtaccagtcc cgtcgaggtc
tcgttgcagt caagaggggt attgcgggta atgaagttga 9660tcttgctgat
ggagattacc aagtggtacc tcttctgccg acttgcaaag atataaacat
9720ggtgaaggtg gcttgcaacg tactactcag caagttcata gtagggccac
caggttccgg 9780gaagaccacc tggctactaa gtcaagttca ggacgatgat
gtcatttaca cacccactca 9840tcagaccatg tttgacatag ttagtgctct
caaagtttgc aggtattcca ttccaggagc 9900ctcgggactc cctttcccac
cgcctgccag gtccgggccg tgggttaggc tcattgccag 9960cgggcacgtc
cctggccgag tatcatacct cgatgaggcc ggatattgca atcatctgga
10020cattcttaga ctgctttcca aaacacccct tgtgtgtttg ggtgaccttc
agcaacttca 10080cccagtcggc tttgattcct attgttatgt gttcgatcag
atgcctcaga agcagttgac 10140caccatttac agatttggcc ccaacatctg
cgcagccatc cagccttgtt acagggagaa 10200acttgaatct aaggctagga
acaccagggt ggtttttacc acccggcctg tggcctttgg 10260ccaggtgctg
acaccatatc acaaagatcg cgtcggctcc gcgattacca tagactcatc
10320ccagggggcc acctttgaca ttgtaacatt gcatctacca tcgccaaagt
ccctaaataa 10380gtcccgggca cttgtggcca tcacacgggc aagacacggg
ttgttcattt atgaccctca 10440caaccagctc cgggagtttt tcaacctaac
ccctgagcgc actgattgta accttgtgtt 10500cagccgtgga gatgagctgg
tggtcctgaa tgcagataat gcagtcacaa ccgtggcgaa 10560ggccttagag
acaggtccaa ctcaatttcg agtgtcagac ccgaggtgca agtctctctt
10620agccgcttgc tcggccagtc tggaagggag ctgcatgccg ctaccgcaag
tggcgcataa 10680cctggggttt tacttctccc cagacagtcc agtatttgca
cctctgccaa aagagttggc 10740gccacattgg ccagtggtta cccatcagaa
taatcgggcg tggcctgatc gacttgtcgc 10800tagtatgcgt ccaattgacg
cccgctacag caagccgatg gtcggtgcag ggtatgtggt 10860cggaccgtcc
accttccttg gtactcctgg agtggtgtca tactatctca cactatacat
10920caggggtgag ccccaggcct tgccagaaac acttgtttca acaggacgta
tagccacaga 10980ttgtcgggag tatctcgacg cggctgagga agaggcagca
aaagaacttc cccacgcgtt 11040cattggcgat gtcaaaggca ccacagttgg
ggggtgtcat cacattacat caaaatacct 11100acctaggtcc ctgcctaaag
actctgttgc cgtagttgga gtaagttcgc ctggcagggc 11160tgctaaagcc
gtatgcaccc tcaccgatgt gtacctccct gaactccggc catatctgca
11220acctgagacg gcatcaaaat gctggaaact caaattagac ttcagggacg
tccgactaat 11280ggtctggaaa ggagccaccg cctactttca attggaaggg
ctcacatggt cggcgctgcc 11340tgactatgcc aggtttattc agctgcccaa
aaacgctgtt gtatacatcg atccgtgcat 11400aggaccggca acagccaatc
gtaaagtcgt acgaaccaca gattggcggg ccgacctggc 11460agtgacgccg
tatgattacg gtgcccggaa cattttgaca acagcctggt tcgaggacct
11520cgggccgcag tggaagattc tggggttgca gccctttagg cgggcgtttg
gctttgaaaa 11580cactgaggat tgggcaatcc ttgcatgctg catgagtgac
ggcaaggact acactgacta 11640taactggaat tgcgttcgac aacgcccaca
cgctatccat ggacgcgctc gtgaccatac 11700gtaccacttt gcccctggca
ctgaattgca agtggagctc ggtaaacccc ggctgccacc 11760tgagcaagta
ccgtgaattc ggagtgatgc aatggggtca ctgtggagta aaatcagcca
11820gctgttcgtg gacgccttca ctgaattcct tgttagtgtg gttgatattg
tcatcttcct 11880tgccatattg tttgggttca ccgtcgcagg atggttactg
gtctttcttc tcagagtggt 11940ttgctccgcg cttctccgtt cgcgctctgc
cattcactct tccgaactat cgaaggtcct 12000atgagggctt actacctaat
tgcagaccgg atgttccaca atttgcattt aagcaccctt 12060tgggtatgtt
ttggcacatg cgggtttccc acctaattga tcagatggtc tctcgccgca
12120tctaccagac catggaacat tcaggtcaag cggcctggaa gcacgtggtc
agtgaggcta 12180ctcttacaaa attgtcagaa ctcgacatag ttctccactt
ccaacacctg gccgcagtgg 12240aggcggactc ttgtcgcttc ctcagctcac
gacttgtgat gctgaaaaat cttgccgttg 12300gcaatgtgag cttgcagtac
aacaccacgt tgaaccgcgt tgagctcatc ctccccacac 12360caggtacgag
gcccaaattg accgatttca gacaatggct catcagtgtg cacgcttcca
12420ttttttcctc tgtagcctca tcagttactt tgttcatagt gctttggctt
cgaattccag 12480ccgtacgcta tgtttttggt ttccattggc ccatggcaac
acgtcattcg agctgaccat 12540taattacact atatgcatgc cctgtcttac
cagccaagcg gctcaacaaa ggctcgaacc 12600cggtcgtaac atgtggtgca
aaataggaca caccacgtgc gaggagcgtg accatgatga 12660gttgtcaatg
tccatcccgt ccgggtacga caacctcaaa cttgaaggtt attacgcttg
12720gctggctttt ttgtcctttt cctacgcggc ccaattccat ccggagttgt
ttggaatagg 12780gaatgtgtcg cgcgtctttg tggataaacg acaccagttc
atttgtgccg agcatgacgg 12840agataattca accgtatcta ccggacacaa
catctccgca tcatatgcgg catattatca 12900ccaccaaata gacgggggca
attggttcca tttggaatgg ctgcggccgc tcttttcctc 12960ttggctagtg
ctcaacatat catggtttct gaggcgttcg cctgcaagcc ctgtttctcg
13020acgcatctat cagatattaa gaccaatacg accgcggctg ccggtttcat
ggtccttcaa 13080gacatcagtt gcctccgacc tcacagggtc tcagcatcgc
aagagaacat tcccttcgga 13140aagtcgtcac aatgtcgtga agccgtcggt
actccccagt acattacgat gactgctaat 13200gtgaccgacg aatcatattt
gtacaacgcg gacttgctaa tgctttccgc gtgccttttc 13260cacgcctcag
aaatgagcga gaaaggcttc aaagttatct ttggaaacgt ctccggcgtt
13320gtttcagctt gtgtcaattt
cacagattat gtggcccatg taacccaaca tacccaacag 13380catcatctgg
taattgatca cattcggtta ctgcatttcc tgacaccatc tgcaatgagg
13440tgggctacaa ccattgcttg tttgttcgcc attctcttag cgatatgaga
tgttctcaca 13500aattggggcg tttcttgact ccgcactctt gcttctggtg
gctttttttg ctgtgtaccg 13560gcttgtcctg gtcctttgcc gatggcaacg
gcaacagctc gacacgccaa tacatatata 13620acttgacgat atgcgagctg
aatgggaccg tctggttgtc cagtcatttt gattgggcag 13680tcgagacctt
tgtgctttac ccggtggcca ctcatatcct ctcactgggt tttctcacaa
13740caagccattt ttttgatgcg ctcggtctcg gcgctgtgtc cactacggga
tttcttggcg 13800ggcggtatgt acttagcagc gtgtacggcg cctgcgcctt
cgcagcgctt gtatgttttg 13860tcatccgtgc tgctaaaaat tgcatggctt
gccgttatgc ccgcacccgg ttcaccaact 13920tcatcgtgga cgaccggggg
aagatccatc gatggaagtc cccaatagtg gtagagaaat 13980taggcaaagc
tgacatcggc ggcgaccttg tcaccatcaa acatgttgtc ctcgaaggag
14040tcaaagctca acctttgacg aggacatcgg cggagcaatg ggaagcctag
atgatttttg 14100caatgatcct accgccgcac agaagcttgt gctggcattt
agtatcacat acacacctat 14160aatgatatac gccctcaagg tgtcacgcgg
ccggctccta ggactgttac acatcctgat 14220atttctgaac tgttctttca
cgttcggata catgacatac gtgcactttc aatccactaa 14280ccgtgtcgcg
cttactatgg gggcggtcgt tgcccttttg tggggcattt acagctttat
14340agaatcatgg aagtttgtca cttccagatg caggttgtgt tgcctaggcc
ggcgatacat 14400tctggcccct gcccaccacg tagaaagtgc tgcaggcctc
cattcaatcc cagcgtctgg 14460taaccgagca tacgctgtga gaaagcccgg
actaacatca gtgaacggca ctctagtacc 14520aggacttcgg agcctcgtgt
tgggcggcaa acgagctgtt aaacgaggag tggttaacct 14580cgtcaagtat
ggccggtaaa aaccagagcc agaagaaaaa gaaaaacaca gctcctatgg
14640ggagtggcca gccagtcaat caactgtgcc aattgctggg cacaatgata
aagtcccagc 14700gccagcggcc taggggagga caggccaaaa tgaaaaagcc
tgagaagcca catttccccc 14760tagctgctga agatgacatc cggcaccatt
tcacccagac cgagcgttcc ctttgcttgc 14820aatcgatcca gacggccttc
aatcaaggcg caggaactgc gtcgctttca tccagcggga 14880aggtcagttt
tcaggttgag ttcatgctgc cggtcgctca tacggtgcgc ctgattcgcg
14940taacttccac atccgccagt cagggtgcaa gttaatttga tagttaggtg
aatggccgcg 15000attggcgtgt ggcctctgag tcacctattc aattagggcg
atcacatggg ggttagactt 15060aattggcgag aaccatgtga ccgaaattaa
aaaaaaaaaa aaaaaaaaaa aaaaaa 151161415111DNAPorcine reproductive
and respiratory syndrome virus 14atgatgtgta gggtattccc cctacataca
cgacacttct agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg
cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgcgccg
cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180tccggagagc
acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc
240ggtgcatgtg caccccggct gcccgggtat tttggaacgc cggccaagtc
ttttgcacac 300ggtgtctcag tgcgcggtct cttctctctc cagagcttca
ggacactgac ctcggtgcag 360ttggcttgtt ttacaagcct agggacaagc
ttcactggaa agtccctatc ggcatccctc 420aggtggaatg tactccatcc
gggtgctgtt ggctctcagc tgttttccct ttggcgcgta 480tgacctccgg
caatcacaac ttcctccaac gacttgtgaa ggttgctgat gttttgtacc
540gtgacggttg cttggcacct cgacaccttc gtgaactcca agtttacgag
cgcggctgca 600actggtaccc gatcacgggg cccgtgcccg ggatgggttt
gtttgcgaac tccatgcacg 660tatccgacca gccgttccct ggtgccaccc
atgtgttgac taactcgcct ttgcctcaac 720aggcttgtcg gcagccgttc
tgtccatttg aggaggctca ttctagcgtg tacaggtgga 780agaaatttgt
ggttttcacg gactcctccc tcaacggtcg atctcgcatg atgtggacgc
840cggaatccga tgattcagcc gccctggagg tactaccgcc tgagttagaa
cgtcaggtcg 900aaatcctcat tcggagtttt cctgctcatc accctgtcga
cctggccgac tgggagctca 960ctgagtcccc tgagaacggt ttttccttca
acacgtctca ttcttgcggt caccttgtcc 1020agaaccccga cgtgtttgat
ggcaagtgct ggctctcctg ctttttgggc cagtcggtcg 1080aagtgcgctg
ccatgaggaa catctagctg acgccttcgg ttaccaaacc aagtggggcg
1140tgcatggtaa gtacctccag cgcaggcttc aagttcgcgg cattcgtgct
gtagtcgatc 1200ctgatggtcc cattcacgtt gaagcgctgt cttgccccca
gtcttggatc aggcacctga 1260ctctggatga tgatgtcacc ccaggattcg
ttcgcctgac atcccttcgc attgtgccga 1320acacagagcc taccacttcc
cggatctttc ggtttggagc gcataagtgg tatggcgctg 1380ccggcaaacg
ggctcgtgct aagcgtgccg ctaaaagtga gaaggattcg gctcccaccc
1440ccaaggttgc cctgccggtc cccacctgtg gaattaccac ctactctcca
ccgacagacg 1500ggtcttgtgg ttggcatgtc cttgccgcca taatgaaccg
gatgataaat ggtgacttca 1560cgtcccctct gactcagtac aacagaccag
aggatgattg ggcttctgat tatgatcttg 1620ttcaggcgat tcaatgtcta
cgactgcctg ctaccgtggt tcggaatcgc gcctgtccta 1680acgccaagta
ccttataaaa cttaacggag ttcactggga ggtagaggtg aggtctggaa
1740tggctcctcg ctccctttct cgtgaatgtg tggttggcgt ttgctctgaa
ggctgtgtcg 1800caccgcctta tccagcagac gggctaccta aacgtgcact
cgaggccttg gcgtctgctt 1860acagactacc ctccgattgt gttagctctg
gtattgctga ctttcttgct aatccacctc 1920ctcaggaatt ctggaccctc
gacaaaatgt tgacctcccc gtcaccagag cggtccggct 1980tctctagttt
gtataaatta ctattagagg ttgttccgca aaaatgcggt gccacggaag
2040gggctttcat ctatgctgtt gagaggatgt tgaaggattg tccgagctcc
aaacaggcca 2100tggcccttct ggcaaaaatt aaagttccat cctcaaaggc
cccgtctgtg tccctggacg 2160agtgtttccc tacggatgtt ttagccgact
tcgagccagc atctcaggaa aggccccaaa 2220gttccggcgc tgctgttgtc
ctgtgttcac cggatgcaaa agagttcgag gaagcagccc 2280cggaagaagt
tcaagagagt ggccacaagg ccgtccactc tgcactcctt gccgagggtc
2340ctaacaatga gcaggtacag gtggttgccg gtgagcaact gaagctcggc
ggttgtggtt 2400tggcagtcgg gaatgctcat gaaggtgctc tggtctcagc
tggtctaatt aacctggtag 2460gcgggaattt gtccccctca gaccccatga
aagaaaacat gctcaatagc cgggaagacg 2520aaccactgga tttgtcccaa
ccagcaccag cttccacaac gacccttgtg agagagcaaa 2580cacccgacaa
cccaggttct gatgccggtg ccctccccgt caccgttcga gaatttgtcc
2640cgacggggcc tatactctgt catgttgagc actgcggcac ggagtcgggc
gacagcagtt 2700cgcctttgga tctatctgat gcgcaaaccc tggaccagcc
tttaaatcta tccctggccg 2760cttggccagt gagggccacc gcgtctgacc
ctggctgggt ccacggtagg cgcgagcctg 2820tctttgtaaa gcctcgaaat
gctttctctg atggcgattc agcccttcag ttcggggagc 2880tttctgaatc
cagctctgtc atcgagtttg accggacaaa agatgctccg gtggttgacg
2940cccctgtcga cttgacgact tcgaacgagg ccctctctgt agtcgatcct
ttcgaatttg 3000ccgaactcaa gcgcccgcgt ttctccgcac aagccttaat
tgaccgaggc ggtccacttg 3060ccgatgtcca tgcaaaaata aagaaccggg
tatatgaaca gtgcctccaa gcttgtgagc 3120ccggtagtcg tgcaacccca
gccaccaggg agtggctcga caaaatgtgg gatagggtgg 3180acatgaaaac
ttggcgctgc acctcgcagt tccaagctgg tcgcattctt gcgtccctca
3240aattcctccc tgacatgatt caagacacac cgcctcctgt tcccaggaag
aaccgagcta 3300gtgacaatgc cggcctgaag caactggtgg cacagtggga
taggaaattg agtgtgaccc 3360cccccccaaa accggttggg ccagtgcttg
accagatcgt ccctccgcct acggatatcc 3420agcaagaaga tgtcaccccc
tccgatgggc caccccatgc gccggatttt cctagtcgag 3480tgagcacggg
cgggagttgg aaaggcctta tgctttccgg cacccgtctc gcggggtcta
3540tcagccagcg ccttatgaca tgggtttttg aagttttctc ccacctccca
gcttttatgc 3600tcacactttt ctcgccgcgg ggctctatgg ctccaggtga
ttggttgttt gcaggtgtcg 3660ttttacttgc tctcttgctc tgtcgttctt
acccgatact cggatgcctt cccttattgg 3720gtgtcttttc tggttctttg
cggcgtgttc gtctgggtgt ttttggttct tggatggctt 3780ttgctgtatt
tttattctcg actccatcca acccagtcgg ttcttcttgt gaccacgatt
3840cgccggagtg tcatgctgag cttttggctc ttgagcagcg ccaactttgg
gaacctgtgc 3900gcggccttgt ggtcggcccc tcaggcctct tatgtgtcat
tcttggcaag ttactcggtg 3960ggtcacgtta tctctggcat gttctcctac
gtttatgcat gcttgcagat ttggcccttt 4020ctcttgttta tgtggtgtcc
caggggcgtt gtcacaagtg ttggggaaag tgtataagga 4080cagctcctgc
ggaggtggct cttaatgtat ttcctttctc gcgcgccacc cgtgtctctc
4140ttgtatcctt gtgtgatcga ttccaaacgc caaaaggggt tgatcctgtg
cacttggcaa 4200cgggttggcg cgggtgctgg cgtggtgaga gccccatcca
tcaaccacac caaaagccca 4260tagcttatgc caatttggat gaaaagaaaa
tgtctgccca aacggtggtt gctgtcccat 4320acgatcccag tcaggctatc
aaatgcctga aagttctgca ggcgggaggg gccatcgtgg 4380accagcctac
acctgaggtc gttcgtgtgt ccgagatccc cttctcagcc ccatttttcc
4440caaaagttcc agtcaaccca gattgcaggg ttgtggtaga ttcggacact
tttgtggctg 4500cggttcgctg cggttactcg acagcacaac tggttctggg
ccggggcaac tttgccaagt 4560taaatcagac cccccccagg aactctatct
ccaccaaaac gactggtggg gcctcttaca 4620cccttgctgt ggctcaagtg
tctgcgtgga ctcttgttca tttcatcctc ggtctttggt 4680tcacatcacc
tcaagtgtgt ggccgaggaa ccgctgaccc atggtgttca aatccttttt
4740catatcctac ctatggcccc ggagttgtgt gctcctctcg actttgtgtg
tctgccgacg 4800gggtcaccct gccattgttc tcagccgtgg cacaactctc
cggtagagag gtggggattt 4860ttattttggt gctcgtctcc ttgactgctt
tggcccaccg catggctctt aaggcagaca 4920tgttagtggt cttttcggct
ttttgtgctt acgcctggcc catgagctcc tggttaatct 4980gcttctttcc
tatactcttg aagtgggtta cccttcaccc tcttactatg ctttgggtgc
5040actcattctt ggtgttttgt ctgccagcag ccggcatcct ctcactaggg
ataactggcc 5100ttctttgggc aattggccgc tttacccagg ttgccggaat
tattacacct tatgacatcc 5160accagtacac ctctgggcca cgtggtgcag
ctgctgtggc cacagcccca gaaggcactt 5220atatggccgc cgtccggaga
gctgctttaa ctgggcgaac tttaatcttc accccgtctg 5280cagttggatc
ccttctcgaa ggtgctttca ggactcataa accctgcctt aacaccgtga
5340atgttgtagg ctcttccctt ggttccggag gggttttcac cattgatggc
agaagaactg 5400tcgtcactgc tgcccatgtg ttgaacggcg acacagctag
agtcaccggc gactcctaca 5460accgcatgca cactttcaag accaatggtg
attatgcctg gtcccatgct gatgactggc 5520agggcgttgc ccctgtggtc
aaggttgcga aggggtaccg cggtcgtgcc tactggcaaa 5580catcaactgg
tgtcgaaccc ggtatcattg gggaagggtt cgccttctgt tttactaact
5640gcggcgattc ggggtcaccc gtcatctcag aatctggtga tcttattgga
atccacaccg 5700gttcaaacaa acttggttct ggtcttgtga caacccctga
aggggagacc tgcaccatca 5760aagaaaccaa gctctctgac ctttccagac
attttgcagg cccaagcgtt cctcttgggg 5820acattaaatt gagtccggcc
atcatccctg atgtaacatc cattccgagt gacttggcat 5880cgctcctagc
ctccgtccct gtagtggaag gcggcctctc gaccgttcaa cttttgtgtg
5940tctttttcct tctctggcgc atgatgggcc atgcctggac acccattgtt
gccgtgggct 6000tctttttgct gaatgaaatt cttccagcag ttttggtccg
agccgtgttt tcttttgcac 6060tctttgtgct tgcatgggcc accccctggt
ctgcacaggt gttgatgatt agactcctca 6120cggcatctct caaccgcaac
aagctttctc tggcgttcta cgcactcggg ggtgtcgtcg 6180gtttggcagc
tgaaatcggg acttttgctg gcagattgtc tgaattgtct caagctcttt
6240cgacatactg cttcttacct agggtccttg ctatgaccag ttgtgttccc
accatcatca 6300ttggtggact ccataccctc ggtgtgattc tgtggttatt
caaataccgg tgcctccaca 6360acatgctggt tggtgatggg agtttttcaa
gcgccttctt cctacggtat tttgcagagg 6420gtaatctcag aaaaggtgtt
tcacagtcct gtggcatgaa taacgagtcc ctaacggctg 6480ctttagcttg
caagttgtca caggctgacc ttgatttttt gtccagctta acgaacttca
6540agtgctttgt atctgcttca aacatgaaaa atgctgccgg ccagtacatt
gaagcagcgt 6600atgccaaggc cctgcgccaa gagttggcct ctctagttca
gattgacaaa atgaaaggag 6660ttttgtccaa gctcgaggcc tttgctgaaa
cagccacccc gtcccttgac ataggtgacg 6720tgattgttct gcttgggcaa
catcctcacg gatccatcct cgatattaat gtggggactg 6780aaaggaaaac
tgtgtccgtg caagagaccc ggagcctagg cggctccaaa ttcagtgttt
6840gtactgtcgt gtccaacaca cccgtggacg ccttgaccgg catcccactc
cagacaccaa 6900cccctctttt tgagaatggt ccgcgtcatc gcagcgagga
agacgatctt aaagtcgaga 6960ggatgaagaa acactgtgta tccctcggct
tccacaacat caatggcaaa gtttactgca 7020aaatttggga caagtctacc
ggtgacacct tttacacgga tgattcccgg tacacccaag 7080accatgcttt
tcaggacagg tcagccgact acagagacag ggactatgag ggtgtgcaaa
7140ccacccccca acagggattt gatccaaagt ctgaaacccc tgttggcact
gttgtgatcg 7200gcggtattac gtataacagg tatctgatca aaggtaagga
ggttctggtc cccaagcctg 7260acaactgcct tgaagctgcc aagctgtccc
ttgagcaagc tctcgctggg atgggccaaa 7320cttgcgacct tacagctgcc
gaggtggaaa agctaaagcg catcattagt caactccaag 7380gtttgaccac
tgaacaggct ttaaactgtt agccgccagc ggcttgaccc gctgtggccg
7440cggcggccta gttgtgactg aaacggcggt aaaaattata aaataccaca
gcagaacttt 7500caccttaggc cctttagacc taaaagtcac ttccgaggtg
gaggtaaaga aatcaactga 7560gcagggccac gctgttgtgg caaacttatg
ttccggtgtc atcttgatga gacctcaccc 7620accgtccctt gtcgacgttc
ttctgaaacc cggacttgac acaatacccg gcattcaacc 7680agggcatggg
gccgggaata tgggcgtgga cggttctatt tgggattttg aaaccgcacc
7740cacaaaggca gaactcgagt tatccaagca aataatccaa gcatgtgaag
ttaggcgcgg 7800ggacgccccg aacctccaac tcccttacaa gctctatcct
gttagggggg atcctgagcg 7860gcataaaggc cgccttatca ataccaggtt
tggagattta ccttacaaaa ctcctcaaga 7920caccaagtcc gcaatccacg
cggcttgttg cctgcacccc aacggggccc ccgtgtctga 7980tggtaaatcc
acactaggta ccactcttca acatggtttc gagctttatg tccctactgt
8040gccctatagt gtcatggagt accttgattc acgccctgac acccctttta
tgtgtactaa 8100acatggcact tccaaggctg ctgcagagga cctccaaaaa
tacgacctat ccacccaagg 8160atttgtcctg cctggggtcc tacgcctagt
acgcagattc atctttggcc atattggtaa 8220ggcgccgcca ttgttcctcc
catcaaccta tcccgccaag aactctatgg cagggatcaa 8280tggccagagg
ttcccaacaa aggacgttca gagcatacct gaaattgatg aaatgtgtgc
8340ccgcgctgtc aaggagaatt ggcaaactgt gacaccttgc accctcaaga
aacagtactg 8400ttccaagccc aaaaccagga ccatcctggg caccaacaac
tttattgcct tggctcacag 8460atcggcgctc agtggtgtca cccaggcatt
catgaagaag gcttggaagt ccccaattgc 8520cttggggaaa aacaaattca
aggagctgca ttgcactgtc gccggcaggt gtcttgaggc 8580cgacttggcc
tcctgtgacc gcagcacccc cgccattgta agatggtttg ttgccaacct
8640cctgtatgaa cttgcaggat gtgaagagta cttgcctagc tatgtgctta
attgctgcca 8700tgacctcgtg gcaacacagg atggtgcctt cacaaaacgc
ggtggcctgt cgtccgggga 8760ccccgtcacc agtgtgtcca acaccgtata
ttcactggta atttatgccc agcacatggt 8820attgtcggcc ttgaaaatgg
gtcatgaaat tggtcttaag ttcctcgagg aacagctcaa 8880gttcgaggac
ctccttgaaa ttcagcctat gttggtatac tctgatgatc ttgtcttgta
8940cgctgaaaga cccacatttc ccaattacca ctggtgggtc gagcaccttg
acctgatgct 9000gggtttcaga acggacccaa agaaaaccgt cataactgat
aaacccagct tcctcggctg 9060cagaattgag gcagggcgac agctagtccc
caatcgcgac cgcatcctgg ctgctcttgc 9120atatcacatg aaggcgcaga
acgcctcaga gtattatgcg tctgctgccg caatcctgat 9180ggattcatgt
gcttgcattg accatgaccc tgagtggtat gaggacctca tctgcggtat
9240tgcccggtgc gcccgccagg atggttatag cttcccaggt ccggcatttt
tcatgtccat 9300gtgggagaag ctgagaagtc ataatgaagg gaagaaattc
cgccactgcg gcatctgcga 9360cgccaaagcc gactatgcgt ccgcctgtgg
gcttgatttg tgtttgttcc attcgcactt 9420tcatcaacac tgccctgtca
ctctgagctg cggtcaccat gccggttcaa aggaatgttc 9480gcagtgtcag
tcacctgttg gggctggcag atcccctctt gatgccgtgc taaaacaaat
9540tccatacaaa cctcctcgta ctgtcatcat gaaggtgggt aataaaacaa
cggccctcga 9600tccggggagg taccagtccc gtcgaggtct cgttgcagtc
aagaggggta ttgcaggcaa 9660tgaagttgat ctttctgatg gggactacca
agtggtgcct cttttgccga cttgcaaaga 9720cataaacatg gtgaaggtgg
cttgcaatgt actactcagc aagttcatag tagggccacc 9780aggttccgga
aagaccacct ggctactgag tcaagtccag gacgatgatg tcatttacac
9840acccacccat cagactatgt ttgatatagt cagtgctctc aaagtttgca
ggtattccat 9900tccaggagcc tcaggactcc ctttcccacc acctgccagg
tccgggccgt gggttaggct 9960tattgccagc gggcacgtcc ctggccgagt
atcatacctc gatgaggctg gatattgtaa 10020tcatctggac attcttagac
tgctttccaa aacacccctt gtgtgtttgg gtgaccttca 10080gcaacttcac
cctgtcggct ttgattccta ctgttatgtg ttcgatcaga tgcctcagaa
10140gcagctgacc actatttaca gatttggccc taacatctgc gcagccatcc
agccttgtta 10200cagggagaaa cttgaatcta aggctaggaa cactagggtg
gtttttacca cccggcctgt 10260ggcctttggt caggtgctga caccatacca
taaagatcgc atcggctctg cgataaccat 10320agattcatcc cagggggcca
cctttgatat tgtgacattg catctaccat cgccaaagtc 10380cctaaataaa
tcccgagcac ttgtagccat cactcgggca agacacgggt tgttcattta
10440tgaccctcat aaccagctcc aggagttttt caacttaacc cctgagcgca
ctgattgtaa 10500ccttgtgttc agccgtgggg atgagctggt agttctgaat
gcggataatg cagtcacaac 10560tgtagcgaag gcccttgaga caggtccatc
tcgatttcga gtatcagacc cgaggtgcaa 10620gtctctctta gccgcttgtt
cggccagtct ggaagggagc tgtatgccac taccgcaagt 10680ggcacataac
ctggggtttt acttttcccc ggacagtcca acatttgcac ctctgccaaa
10740agagttggcg ccacattggc cagtggttac ccaccagaat aatcgggcgt
ggcctgatcg 10800acttgtcgct agtatgcgcc caattgatgc ccgctacagc
aagccaatgg tcggtgcagg 10860gtatgtggtc gggccgtcca cctttcttgg
tactcctggt gtggtgtcat actatctcac 10920actatacatc aggggtgagc
cccaggcctt gccagaaaca ctcgtttcaa cagggcgtat 10980agccacagat
tgtcgggagt atctcgacgc ggctgaggaa gaggcagcaa aagaactccc
11040ccacgcattc attggcgatg tcaaaggtac cacggttggg gggtgtcatc
acattacatc 11100aaaataccta cctaggtccc tgcctaagga ctctgttgcc
gtagttggag taagttcgcc 11160cggcagggct gctaaagccg tgtgcactct
caccgatgtg tacctccccg aactccggcc 11220atatctgcaa cctgagacgg
catcaaaatg ctggaaactc aaattagact tcagggacgt 11280ccgactaatg
gtctggaaag gagccaccgc ctatttccag ttggaagggc ttacatggtc
11340ggcgctgccc gactatgcca ggtttattca gctgcccaag gatgccgttg
tatacattga 11400tccgtgtata ggaccggcaa cagccaaccg taaggtcgtg
cgaaccacag actggcgggc 11460cgacctggca gtgacaccgt atgattacgg
tgcccagaac attttgacaa cagcctggtt 11520cgaggacctc gggccgcagt
ggaagatttt ggggttgcag ccctttaggc gagcatttgg 11580ctttgaaaac
actgaggatt gggcaatcct tgcacgccgt atgaatgacg gcaaggacta
11640cactgactat aactggaact gtgttcgaga acgcccacac gccatctacg
ggcgtgctcg 11700tgaccatacg tatcattttg cccctggcac agaattgcag
gtagagctag gtaaaccccg 11760gctgccgcct gggcaagtgc cgtgaattcg
gggtgatgca atggggtcac tgtggagtaa 11820aatcagccag ctgttcgtgg
acgccttcac tgagttcctt gttagtgtgg ttgatattgc 11880cattttcctt
gccatactgt ttgggttcac cgtcgcagga tggttactgg tctttcttct
11940cagagtggtt tgctccgcgc ttctccgttc gcgctctgcc attcactctc
ccgaactatc 12000gaaggtccta tgaaggcttg ttgcccaact gcagaccgga
tgtcccacaa tttgcagtca 12060agcacccatt gggtatgttt tggcacatgc
gagtttccca cttgattgat gagatggtct 12120ctcgtcgcat ttaccagacc
atggaacatt caggtcaagc ggcctggaag caggtggttg 12180gtgaggccac
tctcacgaag ctgtcagggc tcgatatagt tactcatttc caacacctgg
12240ccgcagtgga ggcggattct tgccgctttc tcagctcacg actcgtgatg
ctaaaaaatc 12300ttgccgttgg caatgtgagc ctacagtaca acaccacgtt
ggaccgcgtt gagctcatct 12360tccccacgcc aggtacgagg cccaagttga
ccgatttcag acaatggctc atcagtgtgc 12420acgcttccat tttttcctct
gtggcttcat ctgttacctt gttcatagtg ctttggcttc 12480gaattccagc
tctacgctat gtttttggtt tccattggcc cacggcaaca catcattcga
12540gctgaccatc aactacacca tatgcatgcc ctgttctacc agtcaagcgg
ctcgccaaag 12600gctcgagccc ggtcgtaaca tgtggtgcaa aatagggcat
gacaggtgtg aggagcgtga 12660ccatgatgag ttgttaatgt ccatcccgtc
cgggtacgac aacctcaaac ttgagggtta 12720ttatgcttgg ctggcttttt
tgtccttttc ctacgcggcc caattccatc cggagttgtt 12780cgggataggg
aatgtgtcgc gcgtcttcgt ggacaagcga caccagttca tttgtgccga
12840gcatgatgga cacaattcaa ccgtatctac cggacacaac atctccgcat
tatatgcggc 12900atattaccac caccaaatag acgggggcaa ttggttccat
ttggaatggc tgcggccact 12960cttttcttcc tggctggtgc tcaacatatc
atggtttctg aggcgttcgc ctgtaagccc 13020tgtttctcga cgcatctatc
agatattgag accaacacga ccgcggctgc cggtttcatg 13080gtccttcagg
acatcaattg tttccgacct cacggggtct cagcagcgca agagaaaatt
13140tccttcggaa agtcgtccca atgtcgtgaa gccgtcggta ctccccagta
catcacgata 13200acggctaacg tgaccgacga
atcatacttg tacaacgcgg acctgctgat gctttctgcg 13260tgccttttct
acgcctcaga aatgagcgag aaaggcttca aagtcatctt tgggaatgtc
13320tctggcgttg tttctgcttg tgtcaatttc acagattatg tggcccatgt
gacccaacat 13380acccagcagc atcatctggt aattgatcac attcggttgc
tgcatttcct gacaccatct 13440gcaatgaggt gggctacaac cattgcttgt
ttgttcgcca ttctcttggc aatatgagat 13500gttctcacaa attggggcgt
ttcttgactc cgcactcttg cttctggtgg ctttttttgc 13560tgtgtaccgg
cttgtcctgg tcctttgccg atggcaacgg cgacagctcg acataccaat
13620acatatataa cttgacgata tgcgagctga atgggaccga ctggttgtcc
agccattttg 13680gttgggcagt cgagaccttt gtgctttacc cggttgccac
tcatatcctc tcactgggtt 13740ttctcacaac aagccatttt tttgacgcgc
tcggtctcgg cgctgtatcc actgcaggat 13800ttgttggcgg gcggtacgta
ctctgcagcg tctacggcgc ttgtgctttc gcagcgttcg 13860tatgttttgt
catccgtgct gctaaaaatt gcatggcctg ccgctatgcc cgtacccggt
13920ttaccaactt cattgtggac gaccggggga gagttcatcg atggaagtct
ccaatagtgg 13980tagaaaaatt gggcaaagcc gaagtcgatg gcaacctcgt
caccatcaaa catgtcgtcc 14040tcgaaggggt taaagctcaa cccttgacga
ggacttcggc tgagcaatgg gaggcctaga 14100cgatttttgc aacgatccta
tcgccgcaca aaagctcgtg ctagccttta gcatcacata 14160cacacctata
atgatatacg cccttaaggt gtcacgcggc cgactcctgg ggctgttgca
14220catcctaata tttctgaact gttcctttac attcggatac atgacatatg
tgcattttca 14280atccaccaac cgtgtcgcac ttaccctggg ggctgttgtc
gcccttctgt ggggtgttta 14340cagcttcaca gagtcatgga agtttatcac
ttccagatgc agattgtgtt gccttggccg 14400gcgatacatt ctggcccctg
cccatcacgt agaaagtgct gcaggtctcc attcaatctc 14460agcgtctggt
aaccgagcat acgctgtgag aaagcccgga ctaacatcag tgaacggcac
14520tctagtacca ggacttcgga gcctcgtgct gggcggcaaa cgagctgtta
aacgaggagt 14580ggttaacctc gtcaagtatg gccggtaaaa accagagcca
gaagaaaaag aaaagtacag 14640ctccgatggg gaatggccag ccagtcaatc
aactgtgcca gttgctgggt gcaatgataa 14700agtcccagcg ccagcaacct
aggggaggac aggccaaaaa gaaaaagcct gagaagccac 14760attttcccct
ggctgctgaa gatgacatcc ggcaccacct cacccagact gaacgctccc
14820tctgcttgca atcgatccag acggctttca atcaaggcgc aggaactgcg
tcgctttcat 14880ccagcgggaa ggtcagtttt caggttgagt ttatgctgcc
ggttgctcat acagtgcgcc 14940tgattcgcgt gacttctaca tccgccagtc
agggtgcaag ttaatttgac agtcaggtga 15000atggccgcga ttggcgtgtg
gcctctgagt cacctattca attagggcga tcacatgggg 15060gtcatactta
atcaggcagg aaccatgtga ccgaaattaa aaaaaaaaaa a
151111515182DNAPorcine reproductive and respiratory syndrome virus
15tttctccacc cctttaacca tgtctgggat acttgatcgg tgcacgtgta cccccaatgc
60cagggtgttt atggcggagg gccaagtcta ctgcacacga tgcctcagtg cacggtctct
120ccttcccctg aacctccaag tttctgagct cggggtgcta ggcctattct
acaggcccga 180agagccactc cggtggacgt tgccacgtgc attccccact
gttgagtgct cccccgccgg 240ggcctgctgg ctctctgcaa tctttccaat
cgcacgaatg accagtggaa acctgaactt 300ccaacaaaga atgatacggg
tcgcagctga gctttacaga gccggccagc tcacccctgc 360agtcttgaag
gctctacaag tttatgaacg gggttgccgc tggtacccca ttgttggacc
420tgcccctgga gtggccgttt acgccaattc cctacatgtg agtgataaac
ctttcccggg 480agcaactcac gtgttgacca acctgccgct cccgcagaga
cccaagcctg aagacttttg 540cccctttgag tgtgctatgg ctactgtcta
tgacattggt catgacgccg tcatgtatgt 600ggccgaaagg aaaatctcct
gggcccctcg tggcggggat gaagtgaaat ttgaagctgt 660ccccggggag
ttgaggttga ttgcgaaccg gctccgcacc tccttcccgc cccaccacac
720agtggacatg tctaagttcg ccttcacagc ccctgggtgt ggtgtttcta
tgcgggttga 780acgccaacac ggctgccttc ccgctgacac tgtccctgaa
ggcaactgct ggtggagctt 840gtttgactcg cttccactgg aagttcagaa
caaagaaatt cgccatgcta accaatttgg 900ctaccagacc aagcatggtg
tctctggcaa gtacctacag cggaggctgc aagttaatgg 960tctccgagca
gtaactgacc taaacggacc tatcgtcgta cagtacttct ccgttaagga
1020gagttggatc cgccatttga aactggcggg agaacccagc tactctgggt
ttgaggacct 1080cctcagaata agggttgagc ctaacacgtc gccattggct
gacaaggaag aaaaaatttt 1140ccggtttggc agtcacaagt ggtacggcgc
tggaaagaga gcaagaaaag cacgctcttg 1200tgcgactgcc acagtcgctg
gccgcgcttt gtccgttcgt gaaacccggc aggccaagga 1260gcacgaggtt
gccggcgcca acaaggctga gcacctcaaa cactactccc cgcctgccga
1320agggaattgt ggttggcatt gcatttccgc catcgccaac cggatggtga
attccaaatt 1380tgaaaccacc cttcccgaaa gagtgagacc tccagatgac
tgggctactg acgaggatct 1440tgtgaatgcc atccaaatcc tcagactccc
tgcggcctta gacaggaacg gtgcttgtac 1500tagcgccaag tacgtactta
agctggaagg tgagcattgg actgtcactg tggcccctgg 1560gatgtcccct
tctttgctcc ctcttgaatg tgttcagggc tgttgtgggc acaagggcgg
1620tcttggttcc ccagatgcag tcgaggtctc cggatttgac cctgcctgcc
ttgaccggct 1680ggctgaggtg atgcacctgc ctagcagtgc tatcccagcc
gctctggccg aaatgtctgg 1740cgattccgat cgttcggctt ctccggtcac
caccgtgtgg actgtttcgc agttctttgc 1800ccgtcacagc ggagggaatc
accctgatca agtgcgctta gggaaaatta tcagcctttg 1860tcaggtgatt
gaggactgct gctgttccca gaacaaaacc aaccgggtca ccccggagga
1920ggtcgcagca aagattgacc tgtacctccg tggtgcaaca aatcttgaag
aatgcttggc 1980caggcttgag aaagcgcgcc cgccacgcgt aatcgacacc
ttctttgatt gggatgttgt 2040gctccctggg gttgaggcgg caacccagac
gatcaagctg ccccaggtca accagtgtcg 2100tgctctggtc cctgttgtga
ctcaaaagtc cttggacgac aactcggtcc ccctgaccgc 2160cttttcactg
gctaactact actaccgtgc gcaaggtgac gaagttcgtc accgtgaaag
2220actaaccgcc gtgctctcca agttggaaaa ggttgttcga gaagaatatg
ggctcatgcc 2280aaccgagcct ggtccacggc ccacactgcc acgcgggctc
gacgaactca aagaccagat 2340ggaggaggac ttgctgaagc tggctaacgc
ccagacgact tcggacatga tggcctgggc 2400agtcgagcag gttgacctaa
aaacttgggt caagaactac ccgcggtgga caccaccacc 2460ccctccgcca
aaagttcagc ctcgaaaaac gaagcctgtc aagagcttgc cggagagaaa
2520gcctgtcccc gccccgcgca ggaaggttgg gtccgattgt ggcagcccgg
tttcattagg 2580cggcgatgtc tctaacagtt gggaagattt ggctgttagt
agcccctttg atctcccgac 2640cccacctgag ccggcaacac cttcaagtga
gctggtgatt gtgtcctcac cgcaatgcat 2700cttcaggccg gcgacaccct
tgagtgagcc ggctccaatt cccgcacctc gcggaactgt 2760gtctcgaccg
gtgacaccct tgagtgagcc gatccctgtg cccgcaccgc ggcgtaagtt
2820tcagcaggtg aaaagattga gttcggcggc ggcaatccca ccgtaccaga
acgagcccct 2880ggatttgtct gcttcctcac agactgaaca tgaggcctct
cccccagcac cgccgcagag 2940cgggggcgtt ccgggagtag aggggcatga
agctgaggaa accctgagtg aaatctcgga 3000catgtcgggt aacattaaac
ctgcgtccgt gtcatcaagc agctccttgt ccagcgtgag 3060aatcacacgc
ccaaaatact cagctcaagc catcatcgac tcgggcgggc cctgcagtgg
3120gcatctccaa gaggtaaagg aaacatgcct tagtgtcatg cgcgaggcat
gtgatgcgac 3180taagcttgat gaccctgcta cgcaggaatg gctttctcgc
atgtgggatc gggtggacat 3240gctgacttgg cgcaacacgt ctgtttacca
ggcgatttgc accttagatg gcaggttaaa 3300gttcctccca aaaatgatac
tcgagacacc gccgccctat ccgtgtgagt ttgtgatgat 3360gcctcacacg
cctgcacctt ccgtaggtgc ggagagcgac cttaccattg gctcagttgc
3420tactgaagat gttccacgca tcctcgagaa aatagaaaat gtcggcgaga
tggccaacca 3480gggacccttg gccttctccg aggataaacc ggtagatgac
caacttgtca acgacccccg 3540gatatcgtcg cggaggcctg acgagagcac
atcagctccg tccgcaggca caggtggcgc 3600cggctctttt accgatttgc
cgccttcaga tggcgcggat acggacgggg gggggccgtt 3660tcggacggca
aaaagaaaag ctgaaaggct ctttgaccaa ctgagccgtc aggtttttga
3720cctcgtctcc catctccctg ttttcttctc acgccttttc taccctggcg
gtggttattc 3780tccgggtgat tggggttttg cagcttttac tctattgtgc
ctctttttat gttacagtta 3840cccagccttt ggtattgctc ccctcttggg
tgtgttttct gggtcttctc ggcgcgttcg 3900aatgggggtt tttggctgct
ggttggcttt tgctgttggt ctgttcaagc ctgtgtccga 3960cccagtcggc
gctgcttgtg agtttgactc gccagagtgt agaaacatcc ttcattcttt
4020tgagcttctc aaaccttggg accctgttcg cagccttgtt gtgggccccg
tcggtctcgg 4080tcttgccatt cttggcaggt tactgggcgg ggcacgctgc
atctggcact ttttgcttag 4140gcttggcatt gttgcagact gtatcttggc
tggagcttac gtgctttctc aaggtaggtg 4200taaaaagtgc tggggatctt
gtataagaac tgctcctaat gaggtcgctt ttaacgtgtt 4260tcctttcaca
cgtgcgacca ggtcgtcact tatcgacctg tgcgatcggt tttgtgcgcc
4320aaaaggaatg gaccccattt ttctcgccac tgggtggcgc gggtgctggg
ccggccgaag 4380ccccattgag caaccctctg aaaaacccat cgcgtttgcc
caattggatg aaaagaagat 4440tacggctagg actgtggtcg cccagcctta
tgaccccaac caagccgtaa agtgcttgcg 4500ggtattgcag gcgggtgggg
cgatggtggc taaggcggtc ccaaaagtgg ttaaggtttc 4560cgctgttcca
ttccgagctc ccttctttcc cactggagtg aaagttgacc ctgattgcag
4620ggtcgtggtt gaccctgaca ctttcactgc agctctccgg tctggctact
ccaccacaaa 4680cctcgtcctt ggtgtagggg actttgccca gctaaatgga
ttaaaaatca ggcaaatttc 4740caagccttca gggggaggcc cacatctcat
ggctgccctg catgttgcct gctcgatggc 4800tctgcacatg cttgctggga
tttatgtgac tgcggtgggt tcttgcggca ccggcaccaa 4860cgacccgtgg
tgcgctaacc cgtttgccgt ccctggctac ggacctggct ctctctgtac
4920gtccagattg tgcatttccc aacacggcct taccctgccc ttgtcagcac
ttgtggcggg 4980attcggtatt caagaaattg ccttggtcgt tttgattttt
gtttccatcg gaggcatggc 5040tcataggttg agctgtaagg ctgacatgct
gtgtgttttg cttgcaattg ccagctatgt 5100ttgggtacct cttacctggt
tgctttgtgt gtttccttgc tggttgcgct gtttttcttt 5160gcaccccctc
accatcctat ggttggtgtt tttcttgatt tctgtgaata tgccttcagg
5220aatcttggcc atggtgttgt tggtttctct ttggcttctt ggtcgttata
ctaatgttgc 5280tggccttgtc accccctacg acattcatca ttacaccagt
ggcccccgcg gtgttgccgc 5340cttggctacc gcaccagatg ggacctactt
ggccgctgtc cgccgcgctg cgttgactgg 5400ccgcaccatg ctgtttaccc
cgtcccagct tgggtctctt cttgagggtg ccttcagaac 5460tcgaaagccc
tcactgaaca ccgtcaatgt gatcgggtcc tccatgggct ctggcggggt
5520gtttaccatc gacgggaaag tcaagtgcgt aactgccgca catgtcctta
cgggcaattc 5580agctcgggtt tccggggtcg gcttcaatca aatgcttgac
tttgacgtaa agggagattt 5640cgctatcgct gattgcccga attggcaagg
ggctgccccc aagacccaat tctgcacgga 5700tggatggact ggccgtgcct
attggctaac atcctctggc gtcgaacccg gcgtcattgg 5760aaaaggattc
gccttctgct tcaccgcatg tggcgattcc gggtccccag tgatcaccga
5820ggccggtgag cttgtcggcg ttcacacggg atcgaataaa caaggggggg
gcattgttac 5880gcgcccctca ggccagtttt gtaatgtggc acccatcaag
ctaagcgaat taagtgaatt 5940ctttgctggg cctaaggtcc cgctcggtga
tgtgaaggtc ggcagccaca taattaaaga 6000cataagcgag gtgccttcag
atctttgtgc cttgcttgct gccaaacctg aactggaagg 6060aggcctctcc
accgtccaac ttctttgtgt gttttttctc ctgtggagaa tgatgggaca
6120tgcctggacg cccttggttg ctgtgagttt ctttattttg aatgaggttc
tccctgccgt 6180cctggtccgg agtgttttct cctttggaat gtttgtgcta
tcctggctca cgccatggtc 6240tgcgcaagtt ctgatgatca ggcttctgac
agcagctctt aacaggaaca gatggtcact 6300tgcctttttc agcctcggtg
cagtgaccgg ttttgtcgca gatcttgcgg ccactcaggg 6360gcatccgttg
caggcagtga tgaatttgag cacctatgca ttcctgcctc ggatgatggt
6420tgtgacctca ccagtcccag tgatcacgtg tggtgtcgtg cacctacttg
ccatcatttt 6480gtacttgttt aagtaccgtg gcctgcacca tatccttgtt
ggcgatggag tgttctctgc 6540ggctttcttc ttgagatact ttgccgaggg
aaagttgagg gaaggggtgt cgcaatcctg 6600cggaatgaat catgagtctc
tgactggtgc cctcgctatg agactcaatg acgaggactt 6660ggatttcctt
atgaaatgga ctgattttaa gtgctttgtt tctgcgtcca acatgaggaa
6720tgcagcgggt caatttatcg aggctgccta tgctaaagca cttagagtag
aactggccca 6780gttggtacag gttgataaag ttcgaggtac tttggccaaa
cttgaagctt ttgctgatac 6840cgtggctcct caactctcgc ccggtgacat
tgttgtcgct ctcggccaca cgcctgttgg 6900cagtatcttc gacctaaagg
ttggtagcac caagcatacc ctccaagcca ttgagaccag 6960agtccttgct
gggtccaaaa tgaccgtggc gcgcgtcgtc gacccgaccc ccacgccccc
7020acccgcaccc gtgcccatcc ccctcccacc gaaagttctg gagaatggcc
ccaacgcttg 7080gggggatgag gaccgtttga ataagaagaa gaggcgcagg
atggaagccc tcggcatcta 7140tgttatgggc gggaaaaagt accagaaatt
ttgggacaag aattccggtg atgtgtttta 7200tgaggaggtc cataataaca
cagatgagtg ggagtgtctc agagttggcg accctgccga 7260ctttgaccct
gagaagggaa ctctgtgtgg acatgtcacc attgaaaata aggcttacca
7320tgtttacacc tccccatctg gtaagaagtt cttggtcccc gtcaacccag
agaatggaag 7380agttcaatgg gaagctgcaa agctttccgt ggagcaggcc
ctaggtatga tgaatgtcga 7440cggcgaactg actgccaaag aactggagaa
actgaaaaga ataattgaca aactccaggg 7500cctgactaag gagcagtgtt
taaactgcta gccgccagcg acttgacccg ctgtggtcgc 7560ggcggcttgg
ttgttactga aacagcggta aaaatagtca aatttcacaa ccggaccttc
7620accctgggac ctgtgaattt aaaagtggcc agtgaggttg agctaaaaga
cgcggttgag 7680cacaaccaac acccggttgc gagaccgatc gatggtggag
ttgtgctctt gcgttccgcg 7740gttccttcgc ttatagacgt cttgatctcc
ggtgctgatg catctcccaa gttacttgcc 7800catcacgggc cgggaaacac
tgggatcgat ggcacgctct gggattttga gtccgaagcc 7860actaaagagg
aagtcgcact cagtgcgcaa ataatacagg cttgtgacat taggcgcggc
7920gacgctcctg aaattggtct cccttacaag ctgtaccctg ttaggggtaa
ccctgagcgg 7980gtgaaaggag ttctgcagaa tacaaggttt ggagacatac
cttacaaaac ccccagtgac 8040actggaagcc cagtgcacgc ggctgcctgc
cttacgccca acgccactcc ggtgactgat 8100gggcgctccg tcttggccac
gaccatgccc cccgggtttg agttatatgt accgaccata 8160ccagcgtctg
tccttgatta ccttgactct aggcctgact gccctaaaca gctgacagag
8220cacggctgcg aagatgccgc actgaaagac ctctctaaat atgacttgtc
cacccaaggc 8280tttgttttac ctggagttct tcgccttgtg cggaaatacc
tgtttgccca tgtaggtaag 8340tgcccacccg ttcatcggcc ttctacttac
cctgctaaga attctatggc tggaataaat 8400gggaacaggt tcccaaccaa
ggacattcag agcgtccctg aaatcgacgt tctgtgcgca 8460caggctgtgc
gagaaaactg gcaaactgtc accccttgta ctcttaagaa acagtattgc
8520gggaagaaga agactaggac catactcggc accaataact tcatcgcact
agcccaccga 8580gcagtgttga gtggtgttac ccagggcttc atgaaaaagg
cgtttaactc gcccatcgcc 8640ctcggaaaga acaagtttaa ggagctacag
actccggtcc tgggcaggtg ccttgaagct 8700gatctcgcat cctgcgatcg
atccacgcct gcaattgtcc gctggtttgc cgccaacctt 8760ctttatgaac
ttgcctgtgc tgaagagcat ctaccgtcgt acgtgctgaa ctgctgccac
8820gacttactgg tcacgcagtc cggcgcagtg actaagagag gtggcctgtc
gtctggcgac 8880ccgatcacct ctgtgtctaa caccatttat agtttggtga
tctatgcaca gcatatggtg 8940cttagttact tcaaaagtgg tcacccccat
ggccttctgt tcttacaaga ccagctaaag 9000tttgaggaca tgctcaaggt
tcaacccctg atcgtctatt cggacgacct cgtgctgtat 9060gccgagtctc
ccaccatgcc aaactatcac tggtgggttg aacatctgaa tttgatgctg
9120gggtttcaga cggacccaaa gaagacagca ataacagact cgccatcatt
tctaggctgt 9180agaataataa atgggcgcca gctagtcccc aaccgtgaca
ggatcctcgc ggccctcgcc 9240tatcacatga aggcgagtaa tgtttctgaa
tactatgcct cagcggctgc aatactcatg 9300gacagctgtg cttgtttgga
gtatgatcct gaatggtttg aagaacttgt agttggaata 9360gcgcagtgcg
cccgcaagga cggctacagt tttcccggca cgccgttctt catgtccatg
9420tgggaaaaac tcaggtccaa ttatgagggg aagaagtcga gagtgtgcgg
gtactgcggg 9480gccccggccc cgtacgctac tgcctgtggc ctcgacgtct
gcatttacca cacccacttc 9540caccagcatt gtccagtcac aatctggtgt
ggccatccag cgggttctgg ttcttgtagt 9600gagtgcaaat cccctgtagg
gaaaggcaca agccctttag acgaggtgct ggaacaagtc 9660ccgtataagc
ccccacggac cgttatcatg catgtggagc agggtctcac cccccttgat
9720ccaggtagat accaaactcg ccgcggacta gtctctgtca ggcgtggaat
taggggaaat 9780gaagttgaac taccagacgg tgattatgct agcaccgcct
tgctccctac ctgcaaagag 9840atcaacatgg tcgctgtcgc ttccaatgta
ctgcgcagca ggttcatcat cggcccaccc 9900ggtgctggga aaacatactg
gctccttcaa caggtccagg atggtgatgt tatttacaca 9960ccaactcacc
agaccatgct tgacatgatt agggctttgg ggacgtgccg gttcaacgtc
10020ccggcaggca caacgctgca attccccgtc ccctcccgca ccggtccgtg
ggttcgcatc 10080ctagccggcg gttggtgtcc tggcaagaat tccttcctag
atgaagcagc gtattgcaat 10140caccttgatg ttttgaggct tcttagtaaa
actaccctca cctgtctagg agacttcaag 10200caactccacc cagtgggttt
tgattctcat tgctatgttt ttgacatcat gcctcaaact 10260caactgaaga
ccatctggag gtttggacag aatatctgtg atgccgttca gccagattac
10320agggacaaac tcatgtccat ggtcaacaca acccgtgtga cctacgtgga
aaaacctgtc 10380aggtatgggc aggtcctcac cccctaccac agggaccgag
aggacgacgc catcactatt 10440gactccagtc aaggcgccac attcgatgtg
gttacattgc atttgcccac taaagattca 10500ctcaacaggc aaagagccct
tgttgccatc accagggcaa gacacgctat ctttgtgtat 10560gacccacaca
ggcagctgca gggcttgttt gatcttcctg caaaaggcac acccgtcaac
10620ctcgcagtgc accgcgacgg gcagctgatc gtgctggata gaaataacaa
agaatgcacg 10680gtcgctcagg ctctaggcaa cggggataaa tttagggcca
cagataagcg tgttgtagat 10740tctctccgcg ccatttgtgc tgatctagaa
gggtcgagct ctccgctccc caaggtcgca 10800cacaacttgg gattttattt
ctcacctgat ttaacacagt ttgctaaact cccagtagaa 10860cttgcacctc
actggcccgt ggtgacaacc cagaacaatg aaaagtggcc agatcggctg
10920gttgccagcc ttcgccctat ccataaatac agccgcgcgt gcatcggtgc
cggctatatg 10980gtgggccctt cggtgtttct aggcactcct ggggtcgtgt
catactatct cacaaaattt 11040gttaagggcg aggctcaatt gcttccggag
acggttttca gcaccggccg aattgaggta 11100gactgccggg aatatcttga
tgatcgggag cgagaagttg ctgcgtccct cccacacgct 11160ttcattggcg
acgtcaaagg cactaccgtt ggaggatgtc atcatgtcac ctccagatac
11220ctcccacgcg tccttcccaa ggaatcagtt gcggtagtcg gggtttcaag
ccccggaaaa 11280gccgcgaaag cattgtgcac actgacagat gtgtacctcc
cagatcttga cgcctatctc 11340cacccggaga cccagtccaa gtgctggaaa
atgatgttgg acttcaaaga agttcgacta 11400atggtctgga aagacaaaac
agcctatttc caacttgaag gtcgctattt cacctggtat 11460cagcttgcca
gctatgcctc gtacatccgt gttcctgtca actctacggt gtacttggac
11520ccctgcatgg gccccgccct ttgcaacagg agagtcgtcg ggtccaccca
ctggggggct 11580gacctcgcgg tcacccctta tgattacggc gctaaaatta
tcctgtctag cgcgtaccat 11640ggtgaaatgc cccccggata caaaattctg
gcgtgcgcgg agttctcgtt ggatgaccca 11700gttaagtaca aacatacctg
ggggtttgaa tcggatacag cgtatctgta tgagttcacc 11760ggaaacggtg
aggactggga ggattacaat gatgcgtttc gtgcgcgcca ggaagggaaa
11820atttataagg ccactgccac cagcttgaag ttttattttc ccccgggccc
tgtcattgaa 11880ccaactttag gcctgaattg aaatgaaatg gggtccatgc
aaagcctttt ttacaaaatt 11940ggccaacttt ttgtggatgc tttcacggag
ttcttggtgt ccattgttga tatcactata 12000tttttggcca ttttgtttgg
cttcaccatc gccggttggc tggtggtctt ttgcatcaga 12060ttggtttgct
ccgcgatact ccgtacgcgc tctgccattc actctgagca attacagaag
12120atcttatgag gcctttcttt cccagtgcca agtggacatt cccacctggg
gaactaaaca 12180tcctttgggg atgctttggc accataaggt gtcaaccctg
attgatgaaa tggtgtcgcg 12240tcgaatgtac cgcatcatgg aaaaagcagg
gcaggctgcc tggaaacagg tggtgagcga 12300ggctacgctg tctcgcatta
gtagtttgga tgtggtggct cattttcagc atctagccgc 12360cattgaagcc
gagacctgta aatatttggc ctcccggctg cccatgctac acaacctgcg
12420catgacaggt tcaaatgtaa ccatagtgta taatagcact ttgaatcagg
tgtttgctat 12480ttttccaacc cctggttccc ggccaaagct taatgatttt
cagcaatggt taatagctgt 12540acattcctcc atattttcct ctgttgcaac
ttcttgtact ctttttgttg tgctgtggtt 12600gcgggttcca atactacgta
ctgcttttgg tttccgctgg ttaggggcaa tttttctttc 12660gaactcacag
tgaattacac ggtgtgtcca ccttgcctca cccggcaagc agccgcagag
12720atctacgaac ccggtaggtc tctttggtgc aggatagggt atgaccgatg
tgaggaggat 12780gatcatgacg agctagggtt tatggtaccg cctggcctct
ccagcgaagg ccacttgact 12840agtgtttacg cctggttggc gttcttgtcc
ttcagctaca cggcccagtt ccatcccgag 12900atattcggga tagggaatgt
gagtcgagtt tatgttgaca tcaaacatca actcatctgc 12960gccgaacatg
acgggcagaa caccaccttg cctcgtcatg acaacatttc agccgtgttt
13020cagacctatt accaacatca agtcgacggc ggcaattggt ttcacctaga
atggcttcgt 13080cccttctttt cctcgtggtt
ggttttaaat gtctcttggt ttctcaggcg ttcgcctgca 13140aaccatgttt
cagttcgagt cttgcagata ttaagaccaa caccaccgca gcggcaagct
13200ttgctgtcct ccaagacatc agttgcctta ggcatcgcga ctcggcctct
gaggcgattc 13260gcaaaatccc tcagtgccgt acggcgatag ggacacccgt
gtatgttacc atcacagcca 13320atgtgacaga tgagaattat ttacattctt
ctgatctcct catgctttct tcttgccttt 13380tctatgcttc tgagatgagt
gaaaagggat ttaaggtggt atttggcaat gtgtcaggca 13440tcgtggctgt
gtgtgtcaat tttaccagct acgtccaaca tgtcaaggag tttacccaac
13500gctccctggt ggtcgaccat gtgcggttgc tccatttcat gacacctgag
accatgaggt 13560gggcaactgt tttagcctgt ctttttgcca ttctgttggc
aatttgaatg tttaagtatg 13620ttggagaaat gcttgaccgc gggctgttac
tcgcaattgc tttctttgtg gtgtatcgtg 13680ccgttctgtt ttgctgtgct
cgtcaacgcc agcaacgaca gcagctccca tctacagctg 13740atttacaact
tgacgctatg tgagctgaat ggcacagatt ggctagctaa caaatttgat
13800tgggcagtgg agagttttgt catctttccc gttttgactc acattgtctc
ctatggtgcc 13860ctcactacta gccatttcct tgacacagtc gctttagtca
ctgtgtctac cgccgggttt 13920gttcacgggc ggtatgtcct aagtagcatc
tacgcggtct gtgccctggc tgcgttgact 13980tgcttcgtca ttaggtttgc
aaagaattgc atgtcctggc gctacgcgtg taccagatat 14040accaactttc
ttctggacac taagggcaga ctctatcgtt ggcggtcgcc tgtcatcata
14100gagaaaaggg gcaaagttga ggtcgaaggt catctgatcg acctcaaaag
agttgtgctt 14160gatggttccg tggcaacccc tataaccaga gtttcagcgg
aacaatgggg tcgtccttag 14220atgacttctg tcatgatagc acggctccac
aaaaggtgct tttggcgttt tctattacct 14280acacgccagt gatgatatat
gccctaaagg tgagtcgcgg ccgactgcta gggcttctgc 14340accttttgat
cttcctgaat tgtgctttca ccttcgggta catgactttc gcgcactttc
14400agagtacaaa taaggtcgcg ctcactatgg gagcagtagt tgcactcctt
tggggggtgt 14460actcagccat agaaacctgg aaattcatca cctccagatg
ccgtttgtgc ttgctaggcc 14520gcaagtacat tctggcccct gcccaccacg
ttgaaagtgc cgcaggcttt catccgattg 14580cggcaaatga taaccacgca
tttgtcgtcc ggcgtcccgg ctccactacg gtcaacggca 14640cattggtgcc
cgggttaaaa agcctcgtgt tgggtggcag aaaagctgtt aaacagggag
14700tggtaaacct tgtcaaatat gccaaataac aacggcaagc agcagaatag
aaagaagggg 14760gatggccagc cagtcaatca gctgtgccag atgctgggta
agatcatcgc tcagcaaaac 14820cagtccagag gcaagggacc gggaaagaaa
aataagaaga aaaacccgga gaagccccat 14880tttcctctag cgactgaaga
tgatgtcaga catcacttta cccctagtga gcggcaattg 14940tgtctgtcgt
caatccagac cgcctttaat caaggcgctg ggacttgcac cctgtcagat
15000tcagggagga taagttacac tgtggagttt agtttgccta cgcatcatac
tgtgcgcctg 15060attcgcgtca cagcatcacc ctcagcatga tgggctggca
ttcttgaggc atctcagtgt 15120ttgaattgga agaatgtgtg gtgaatggca
ctgattgaca ttgtgcctct aagcactata 15180tt 1518216383PRTPorcine
reproductive and respiratory syndrome virus 16Met Ser Gly Thr Phe
Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn
Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser
Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40
45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly
50 55 60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu
Ser Gly 65 70 75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His
Asn Phe Leu Gln 85 90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr
Arg Asp Gly Cys Leu Thr 100 105 110 Ser Arg His Leu Arg Glu Leu Gln
Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile Thr Gly Pro
Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met His Val Ser
Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn
Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170
175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
180 185 190 Thr Asp Ser Ser Pro Asn Gly Arg Met Met Trp Thr Pro Glu
Ser Asp 195 200 205 Asp Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu
Glu Arg Gln Val 210 215 220 Glu Ile Leu Ile Arg Ser Phe Pro Ala His
His Pro Val Ser Leu Ala 225 230 235 240 Asp Trp Glu Leu Ala Glu Ser
Pro Glu Asn Gly Phe Ser Phe Ser Thr 245 250 255 Tyr His Ser Gly Gly
Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser 260 265 270 Lys Cys Trp
Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu 275 280 285 Tyr
Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly 290 295
300 Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg
305 310 315 320 Ala Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala
Leu Ser Cys 325 330 335 Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp
Asp Asp Val Thr Pro 340 345 350 Gly Phe Val Arg Leu Thr Ser Leu Arg
Ile Val Pro Asn Thr Glu Pro 355 360 365 Thr Thr Leu Arg Ile Phe Arg
Phe Gly Ala His Lys Trp Tyr Gly 370 375 380 17382PRTPorcine
reproductive and respiratory syndrome virus 17Met Ser Gly Thr Phe
Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn
Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser
Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40
45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly
50 55 60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu
Ser Gly 65 70 75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His
Asn Phe Leu Gln 85 90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr
Arg Asp Gly Cys Leu Thr 100 105 110 Ser Arg His Leu Arg Glu Leu Gln
Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile Thr Gly Pro
Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met His Val Ser
Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn
Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170
175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
180 185 190 Thr Asp Ser Ser Pro Asn Gly Met Met Trp Thr Pro Glu Ser
Asp Asp 195 200 205 Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu
Arg Gln Val Glu 210 215 220 Ile Leu Ile Arg Ser Phe Pro Ala His His
Pro Val Ser Leu Ala Asp 225 230 235 240 Trp Glu Leu Ala Glu Ser Pro
Glu Asn Gly Phe Ser Phe Ser Thr Tyr 245 250 255 His Ser Gly Gly Tyr
Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys 260 265 270 Cys Trp Leu
Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr 275 280 285 Glu
Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val 290 295
300 Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala
305 310 315 320 Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu
Ser Cys Pro 325 330 335 Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp
Asp Val Thr Pro Gly 340 345 350 Phe Val Arg Leu Thr Ser Leu Arg Ile
Val Pro Asn Thr Glu Pro Thr 355 360 365 Thr Leu Arg Ile Phe Arg Phe
Gly Ala His Lys Trp Tyr Gly 370 375 380 18381PRTPorcine
reproductive and respiratory syndrome virus 18Met Ser Gly Thr Phe
Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn
Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser
Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40
45 Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly
50 55 60 Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu
Ser Gly 65 70 75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His
Asn Phe Leu Gln 85 90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr
Arg Asp Gly Cys Leu Thr 100 105 110 Ser Arg His Leu Arg Glu Leu Gln
Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile Thr Gly Pro
Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met His Val Ser
Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn
Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170
175 Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
180 185 190 Thr Asp Ser Ser Pro Asn Met Met Trp Thr Pro Glu Ser Asp
Asp Ser 195 200 205 Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg
Gln Val Glu Ile 210 215 220 Leu Ile Arg Ser Phe Pro Ala His His Pro
Val Ser Leu Ala Asp Trp 225 230 235 240 Glu Leu Ala Glu Ser Pro Glu
Asn Gly Phe Ser Phe Ser Thr Tyr His 245 250 255 Ser Gly Gly Tyr Leu
Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys 260 265 270 Trp Leu Ser
Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu 275 280 285 Asp
Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser 290 295
300 Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val
305 310 315 320 Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser
Cys Pro Gln 325 330 335 Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp
Val Thr Pro Gly Phe 340 345 350 Val Arg Leu Thr Ser Leu Arg Ile Val
Pro Asn Thr Glu Pro Thr Thr 355 360 365 Leu Arg Ile Phe Arg Phe Gly
Ala His Lys Trp Tyr Gly 370 375 380 19380PRTPorcine reproductive
and respiratory syndrome virus 19Met Ser Gly Thr Phe Ser Arg Cys
Met Cys Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn Ala Gly Gln
Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser Leu Leu Pro
Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45 Leu Phe
Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60
Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65
70 75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe
Leu Gln 85 90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp
Gly Cys Leu Thr 100 105 110 Ser Arg His Leu Arg Glu Leu Gln Val Tyr
Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile Thr Gly Pro Val Pro
Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met His Val Ser Asp Gln
Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn Ser Pro
Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175 Glu
Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185
190 Thr Asp Ser Ser Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala
195 200 205 Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu
Ile Leu 210 215 220 Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu
Ala Asp Trp Glu 225 230 235 240 Leu Ala Glu Ser Pro Glu Asn Gly Phe
Ser Phe Ser Thr Tyr His Ser 245 250 255 Gly Gly Tyr Leu Val Gln Asn
Pro Asp Val Phe Asp Ser Lys Cys Trp 260 265 270 Leu Ser Cys Phe Leu
Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp 275 280 285 Tyr Leu Ala
Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly 290 295 300 Lys
Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile 305 310
315 320 Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln
Ser 325 330 335 Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro
Gly Phe Val 340 345 350 Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr
Glu Pro Thr Thr Leu 355 360 365 Arg Ile Phe Arg Phe Gly Ala His Lys
Trp Tyr Gly 370 375 380 20379PRTPorcine reproductive and
respiratory syndrome virus 20Met Ser Gly Thr Phe Ser Arg Cys Met
Cys Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn Ala Gly Gln Val
Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser Leu Leu Pro Pro
Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45 Leu Phe Tyr
Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60 Ile
Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65 70
75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu
Gln 85 90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly
Cys Leu Thr 100 105 110 Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu
Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile Thr Gly Pro Val Pro Gly
Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met His Val Ser Asp Gln Pro
Phe Pro Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn Ser Pro Leu
Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175 Glu Glu
Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190
Thr Asp Ser Ser Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp 195
200 205 Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu
Ile 210 215 220 Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp
Trp Glu Leu 225 230 235 240 Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe
Ser Thr Tyr His Ser Gly 245 250 255 Gly Tyr Leu Val Gln Asn Pro Asp
Val Phe Asp Ser Lys Cys Trp Leu 260 265 270 Ser Cys Phe Leu Asp Gln
Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr 275 280 285 Leu Ala Asn Ala
Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys 290 295 300 Tyr Leu
Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp 305 310 315
320 Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp
325 330 335 Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe
Val Arg 340 345 350 Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro
Thr Thr Leu Arg 355 360 365 Ile Phe Arg Phe Gly Ala His Lys Trp Tyr
Gly 370 375 21203PRTPorcine reproductive and respiratory syndrome
virus 21Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser
Ser 1 5 10 15 Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser
Asp Asp Ser Ala Asp 20 25 30 Leu Glu Ala Leu Pro Pro Glu Leu Glu
Arg Gln Val Glu Ile Leu Ile 35 40 45 Arg Ser Phe Pro Ala His His
Pro Val Ser Leu Ala Asp Trp Glu Leu 50 55 60 Ala Glu Ser Pro Glu
Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly 65 70 75 80 Gly Tyr Leu
Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu 85 90 95 Ser
Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr 100 105
110 Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys
115 120 125 Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val
Ile Asp 130 135 140 Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys
Pro Gln Ser Trp 145 150 155 160 Ile Arg His Leu Thr Leu Asp Asp Asp
Val Thr Pro Gly Phe Val Arg 165 170 175 Leu Thr Ser Leu Arg Ile Val
Pro Asn Thr Glu Pro Thr Thr Leu Arg 180 185 190 Ile Phe Arg Phe Gly
Ala His Lys Trp Tyr Gly 195 200 22202PRTPorcine reproductive and
respiratory syndrome virus 22Ser Ser Val Tyr Arg Trp Lys Lys Phe
Val Val Phe Thr Asp Ser Ser 1 5 10 15 Pro Asn Gly Met Met Trp Thr
Pro Glu Ser Asp Asp Ser Ala Asp Leu 20 25 30 Glu Ala Leu Pro Pro
Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg 35 40 45 Ser Phe Pro
Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala 50 55 60 Glu
Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly Gly 65 70
75 80 Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu
Ser 85 90 95 Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu
Asp Tyr Leu 100 105 110 Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly
Val Ser Gly Lys Tyr 115 120 125 Leu Gln Arg Arg Leu Gln Val Asn Gly
Ile Arg Ala Val Ile Asp Pro 130 135 140 Asp Gly Pro Ile His Val Glu
Ala Leu Ser Cys Pro Gln Ser Trp Ile 145 150 155 160 Arg His Leu Thr
Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu 165 170 175 Thr Ser
Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile 180 185 190
Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195 200 23201PRTPorcine
reproductive and respiratory syndrome virus 23Ser Ser Val Tyr Arg
Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Pro Asn Met
Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu 20 25 30 Ala
Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40
45 Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala Glu
50 55 60 Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly
Gly Tyr 65 70 75 80 Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys
Trp Leu Ser Cys 85 90 95 Phe Leu Asp Gln Pro Ile Glu Val Arg Leu
Tyr Glu Asp Tyr Leu Ala 100 105 110 Asn Ala Phe Gly Tyr Gln Thr Lys
Trp Gly Val Ser Gly Lys Tyr Leu 115 120 125 Gln Arg Arg Leu Gln Val
Asn Gly Ile Arg Ala Val Ile Asp Pro Asp 130 135 140 Gly Pro Ile His
Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg 145 150 155 160 His
Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr 165 170
175 Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile Phe
180 185 190 Arg Phe Gly Ala His Lys Trp Tyr Gly 195 200
24200PRTPorcine reproductive and respiratory syndrome virus 24Ser
Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala
20 25 30 Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg
Ser Phe 35 40 45 Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu
Leu Ala Glu Ser 50 55 60 Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr
His Ser Gly Gly Tyr Leu 65 70 75 80 Val Gln Asn Pro Asp Val Phe Asp
Ser Lys Cys Trp Leu Ser Cys Phe 85 90 95 Leu Asp Gln Pro Ile Glu
Val Arg Leu Tyr Glu Asp Tyr Leu Ala Asn 100 105 110 Ala Phe Gly Tyr
Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu Gln 115 120 125 Arg Arg
Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro Asp Gly 130 135 140
Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg His 145
150 155 160 Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu
Thr Ser 165 170 175 Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu
Arg Ile Phe Arg 180 185 190 Phe Gly Ala His Lys Trp Tyr Gly 195 200
25199PRTPorcine reproductive and respiratory syndrome virus 25Ser
Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu
20 25 30 Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser
Phe Pro 35 40 45 Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu
Ala Glu Ser Pro 50 55 60 Glu Asn Gly Phe Ser Phe Ser Thr Tyr His
Ser Gly Gly Tyr Leu Val 65 70 75 80 Gln Asn Pro Asp Val Phe Asp Ser
Lys Cys Trp Leu Ser Cys Phe Leu 85 90 95 Asp Gln Pro Ile Glu Val
Arg Leu Tyr Glu Asp Tyr Leu Ala Asn Ala 100 105 110 Phe Gly Tyr Gln
Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu Gln Arg 115 120 125 Arg Leu
Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro Asp Gly Pro 130 135 140
Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg His Leu 145
150 155 160 Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr
Ser Leu 165 170 175 Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg
Ile Phe Arg Phe 180 185 190 Gly Ala His Lys Trp Tyr Gly 195
2650PRTPorcine reproductive and respiratory syndrome virus 26Ser
Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp
20 25 30 Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile
Leu Ile 35 40 45 Arg Ser 50 2749PRTPorcine reproductive and
respiratory syndrome virus 27Ser Ser Val Tyr Arg Trp Lys Lys Phe
Val Val Phe Thr Asp Ser Ser 1 5 10 15 Pro Asn Gly Met Met Trp Thr
Pro Glu Ser Asp Asp Ser Ala Asp Leu 20 25 30 Glu Ala Leu Pro Pro
Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg 35 40 45 Ser
2848PRTPorcine reproductive and respiratory syndrome virus 28Ser
Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10
15 Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu
20 25 30 Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile
Arg Ser 35 40 45 2947PRTPorcine reproductive and respiratory
syndrome virus 29Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe
Thr Asp Ser Ser 1 5 10 15 Pro Met Met Trp Thr Pro Glu Ser Asp Asp
Ser Ala Asp Leu Glu Ala 20 25 30 Leu Pro Pro Glu Leu Glu Arg Gln
Val Glu Ile Leu Ile Arg Ser 35 40 45 3046PRTPorcine reproductive
and respiratory syndrome virus 30Ser Ser Val Tyr Arg Trp Lys Lys
Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Met Met Trp Thr Pro Glu
Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu 20 25 30 Pro Pro Glu Leu
Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 45 3115110DNAPorcine
reproductive and respiratory syndrome virus 31atgatgtgta gggtattccc
cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc
caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc
gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt
180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg
acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc
cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc
cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct
aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg
tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca
480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat
gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca
agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg
ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct
ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg
gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga
780aaaaatttgt ggtcttcacg gactcctccc ccaacggtcg gatgatgtgg
acgccggaat 840ccgatgattc agccgaccta gaggcgctac cgcctgagct
agaacgtcag gtcgaaatcc 900tcattcggag ttttcctgcc catcaccctg
tcagcctggc cgactgggag ctcgctgagt 960cccctgagaa cggtttttcc
ttcagcacgt accattctgg tggttatctt gtccaaaacc 1020ccgacgtgtt
tgacagcaag tgctggctct cctgtttctt ggatcagccg atcgaagtgc
1080gcctctatga ggattatctg gctaacgctt tcggttacca aaccaagtgg
ggcgtgtctg 1140gtaagtacct ccagcgcagg cttcaagtca acggtattcg
tgctgtaatc gatcctgatg 1200gccccattca cgttgaagcg ctgtcttgcc
cccaatcttg gatcaggcac ctgactctgg 1260acgatgacgt caccccagga
ttcgttcgcc taacatccct ccgcattgtg ccgaacacag 1320agcccactac
tctccgaatc tttcggtttg gagcgcataa gtggtatggc gctgccggca
1380aacgggctcg tgccaagcgt gccgctaaaa gtgagaaggg tccggctccc
actcccaagg 1440ttgccccgcc agcccccacc tgcggaattg ttacctactc
tccaccaaca gacgggtctt 1500gcggttggca cgtccttgcc gccataatga
accgaatgat gaacggtgac ttcatgtccc 1560ctctggccca gtacaacaga
ccagaggatg attgggcttc tgattacgat cttgcccagg 1620cgattcaatg
tctgcggttg cctgctacca tagttcggaa tcgtgcctgt cccaacgcca
1680agtacctcat aaaactcaac ggagtccact gggaggtaga ggtgaggtca
gggatggccc 1740ctcgctccct tccccgcgag tgcgtagtcg gcgtttgttc
cgaaggctgt gccgcatcgc 1800cttacccaga aaacgggcta cctaaacgag
cgtttgaggc cttggcgtct gcttacagac 1860taccttccga ttgtgtcagt
tctggtattg ctgactttct tgctaacccc cctcaggaat 1920tctggactct
tgacaaaatg ttgacctccc cgtcaccgga gcggtctggt ttttccagtc
1980tgtacaaatt gctattagag gttgtcccgc agaaatgcgg agccacggaa
ggggctttca 2040cctatgctgt tgagagaatg ctgaaggatt gcccgagctc
cgaacaggcc atggcccttc 2100tggcaaaaat taaagttcca tcctcaaagg
ccccgtctgt gtccctggac gagtgtttcc 2160ctacggatgt ttcagccgat
ttcgaaccag catctcagga gaggtcccaa aattccagcg 2220ctgctgttgt
cctgcattca ccgaatgcaa aagagttcga ggaagcagct ccaggggaag
2280ttcaggaggg tggccacaag gccgtccact ctgcactccc tgccgggggt
cctaacaata 2340agcaggcaca gctggttgcc ggtgagcaac tgaagctcgg
cggttgtggt tcggtagttg 2400ggaatgcaca tgaaggtgtt ctggtcccac
ctggtccaat taatttgaca agcggggatt 2460taccctcctc aggctccatg
aaagaagata tgctcaatag ccgggaggac gaaccactgg 2520atttgtccca
accagcaaca gctgtcacaa cgactcttat gggagagcta acacccgact
2580acctaggttc tgatactggt gccctccccg tcaccgtccg aaaatttgtc
ccgacggggc 2640ctatactccg tcatgttgag cactgcagca cggggtcggg
cgatagcagt tcgcctttgg 2700atctgtctgg tgcgcaaacc ccggaccagc
ctttaaatct gtccctggcg gcttggccag 2760tgaggaccac cgcgtctgat
cctggctggg tccacggtag acgcgagcct gtctttgtaa 2820agcctcgaga
tgttttctct gatggcgatt cagcccttca gttcggggag ctttctgaat
2880ccagctctgt catcgagttt gatcgggcaa aagatgctca ggtggctgac
gcccctgtcg 2940gtctgacgac ttcggacgag gccctctccg cagtcgatcc
tttcgagttt tccgaactca 3000agcgcccacg tttctccgca caagccttaa
ttgaccgagg cggcccactt gccgatgtcc 3060atgcaaaaat aaagaaccgg
gtatatgaac agtgcctcca agcttgtgag cccggtagtc 3120gcgcaacccc
agctaccagg gagtggctcg acaaaatgtg ggagagagtg gacatgaaaa
3180cctggcgctg cacctcacag ttccaagctg gtcacattct tgcgtccctc
aaattcctcc 3240ctgacatgat tcaagacaca ccgcctcctg ttcccaggaa
gaaccgagcc agtgataaag 3300ctggcctgaa acaactagtg gcacagtggg
ataggagatt gagttcaacc ccccccccaa 3360aaccggttgg gccggtactt
gaccgggtcg atcctccgcc tacgggtacc cggcaagaag 3420acgtcacccc
ctccgatggg ccaccccatg cgccggatgg tcgagtgagt acgggtggga
3480gttggaaagg ccttatgctt tccggcaccc gtctcacggg gtccatcagt
catcgcctca 3540tgacatgggt ttttgaagtt gtctcccatc tcccagcttt
tatgctcaca cttttctcgc 3600cgcggggctc tatggctcca ggcgattggg
tttttgcagg tgttgtttta cttgctctcc 3660tgctctgtcg ttcttaccca
atattcgggt gccttccctt attgggtgtc ttttctggta 3720ctgtgcggcg
tgttcgtctg ggtgtttttg gctcttggat ggcttttgct gtatttttat
3780tctcgactcc atccaaccca gtcggttctt cttgtaacca cgattcgccg
gagtgtcatg 3840ctgagcttct ggctcttgag cagcgccaac tttgggaacc
tgtgcgcggc cttgtggtcg 3900gcccatcggg cctcttatgt gtcattcttg
gcaagttact cggtgggtca cgttatctct 3960ggcatgttct cctacgtcta
tgcctgcttg cagatttggc cctttctctt gtttatgtgg 4020tgtcccaggg
gcgttgtcac aagtgttggg gaaagtgtat aaggacagct cctgctgagg
4080tggcttttaa tgtatttcct ttctcgcgcg ccacccgtag ttctcttgta
tccttatgtg 4140atcgattcca aacgccaaaa ggagttgatc ccgtgcactt
ggcgacaggt tggcgcgggt 4200gctggcgtgg tgagagtcct atccatcaac
cacaccaaaa gcccatagct tacgccaatt 4260tggatgaaaa gaagatatct
gcccaaacgg ttgttgctgt cccatacgat cccaatcagg 4320ctatcaaatg
tctgaaagtt ctgcaggcgg gaggggctat cgtggatcag cctacgcctg
4380aggtcgttcg tgtgtccgag atccccttct cagccccatt tttcccaaaa
gttccagtca 4440acccagattg tagggtcgtg gtggattcgg acacttttgt
ggctgcggtt cgttgtggtt 4500attcgacatc acaactggtc ctgggccagg
gcaactttgc caagttaaat caaacccccc 4560ccaggaactc tatctccacc
aaagcgactg gtggggcctc ttatactttt gctgtggctc 4620aagtgtctgt
gtggaccctt gttcatttcg tcctcggtct ttggctcacg tcacctcaag
4680tgtgtggtcg aggaaccgct gacccatggt gttcaagtcc attttcatat
cctacctatg 4740gccccggggt tgtgtgctcc tctcgacttt gtgtgtctgc
tgacggggtc acccttccat 4800tgttctcagc cgtggcacaa ctctctggta
gggaggtggg gatttttatt ttagtgctcg 4860tctcctttat tgccttggcc
caccgcatgg ctcttaaggc agacatgtta gtagtctttt 4920tggctctttg
tgcttatgcc tggcccatga gctcctggtt gatctgcttc tttcctatac
4980tcttgaggtg ggttaccctt caccctctca ctatgctttg ggtgcattca
ttcttgatgt 5040tttgtctccc agcagccggc gtcctctcac tggggataac
tggcctcctc tgggcaatcg 5100gccgctttac tcaggttgcc ggaatcatta
caccttatga tatccaccag tacacctctg 5160ggccacgtgg tgcagccgct
gtggccacag ccccagaagg cacttacatg gccgccgtcc 5220ggagagctgc
tttaaccggg cggactttaa tcttcacccc gtccgcagtt ggatcccttc
5280tcgaaggtgc tttcaggact cataaaccct gccttaacac cgtgaatgtc
gtaggctcct 5340cccttggttc cggaggggtt ttcaccattg acggaaaaaa
aattgtcgtc actgctgccc 5400atgtgctgaa cggcgacaca gctagagtca
ccggtgattc ctacaaccgc atgcacactt 5460tcaagactaa tggtgactat
gcctggtccc atgctgataa ctggcagggc gctgcccctg 5520tggtcaaggt
tgcgaaaggg tatcgcggtc gtgcctactg gcaaacatca actggtgtcg
5580agcctggtgt tattgggaat gggttcgcct tctgtttcac caactgcggc
gattcggggt 5640cacccgttat ctcagaatct ggtgatctta tcggaatcca
caccggttca aacaaacttg 5700gttctggtct tgtgacaacc cctgaagggg
agacctgtac catcagagaa accaagcttt 5760ctgacctttc cagacatttc
gcaggcccaa gcgttcctct tggggacatc aaattgagtc 5820cggccatcat
ccctgatgtg acatccattc cgagtgactt ggcatcgctc ctagcttccg
5880tccctgtaat ggaaggcggc ctctcgaccg ttcaactttt gtgtgtcttt
ttcctcctct 5940ggcgcatgat gggccatgcc tggacgccca ttgttgccgt
gggcttcttt ttgctgaatg 6000aaattcttcc agcagttttg gttcgagccg
tgttttcttt tgcactcttt gtgcttgcat 6060gggccacccc ctggtctgca
caagtgttga tgattagact tctcacggca tctctcaacc 6120gcaacaaact
ttctcttgcg ttctacgcac tcggaggtgt tgttggtttg gctgctgaaa
6180tcgggacttt tgctggtaaa ttgtctgaat tgtctcaagc tctttcgaca
tactgtttct 6240tacctagggt ccttgctatg accagctgtg ttcccatcat
catcattggt ggactccatg 6300ccctcggtgt gattctgtgg ttattcaaat
accggtgcct ccacaacatg ctggttggtg 6360atggaagctt ttcaagcgct
ttcttcctac ggtattttgc agagggcaat ctcaggagag 6420gtgtttcaca
gtcctgtggc atgagtaacg agtccctgac ggctgctttg gcttgcaagt
6480tgtcacaggc tgaccttgat tttttgtcca gcttaacgaa
cttcaagtgc tttgtatctg 6540cttcaaacat gaaaaatgct gccggccagt
acattgaagc agcttatgcc agggccctgc 6600gtcaagagtt ggcctcttta
gtccagattg acaaaatgaa aggagttttg tccaagctag 6660aggcctttgc
tgaaacggcc actccgtccc tcgacgtagg tgacgtgatt gttctacttg
6720gacaacatcc tcacggatcc gttctcgata ttaatgtggg gactgaaagg
aaaactgtat 6780ccgtgcaaga gacccggagc ctaggcggct ccaagttcag
tgtttgtact gttgtgtcaa 6840acacacccgt ggacgcctta gccggtattc
cactccagac accaaccccc cttttcgaga 6900atggcccgcg tcatcgcagc
gaggaggacg atcttaaagt cgagaggatg aagaaacact 6960gcgtgtccct
cggcttccac aacattaacg gtaaagttta ctgcaagatt tgggacaagt
7020ctaccggtga cgccttttac actgatgatt cccggtacac ccaagactat
gcttttcagg 7080acaggtcagc tgactataga gacagggact acgagggtgt
gcaaaccgcc ccccaacagg 7140gatttgatcc aaagtctgaa acccctgttg
gtaccgttgt gatcggcggt attacgtaca 7200acaggtattt ggtcaaaggt
aaggaggttc tggttcccaa gcctgacaac tgccttgaag 7260ctgccaagct
gtcccttgag caagctctcg ctgggatggg ccaaacttgt gaccttacag
7320ctgccgaggt ggaaaagcta aagcgcatca ttggtcaact tcaaggattg
accactgagc 7380aggctttaaa ctgttagccg ccagcggctt gacccgctgt
ggccgcggcg gcctagttgt 7440aactgaaacg gcggtaaaaa ttgtcaaata
ccacagcaga acttttacct taggctcttt 7500agacctaaaa gtcacttccg
aggtggaggt gaagaagtca accgagcagg gccacgctgt 7560tgtggcaaac
ttgtgttctg gtgtcgtctt gatgagacct cacccaccgt cccttgtcga
7620cgttcttctg aaacccggac ttgacataac acccggcatt caaccagggc
atggggccgg 7680gaatatgggc gtggacggtt ccatttggga ttttgaaacc
gcacccacaa aggctgaact 7740cgagttatcc aagcaaataa ttcaagcatg
tgaagtcagg cgcggggatg ccccgaacct 7800ccagctccct tacaagctct
atcctgttag aggggatcct gagcggcata aaggccacct 7860catcaatacc
aggtttggag acttacctta caaaactcct caagacacca agtccgcaat
7920ccacgcggct tgttgcctgc accccaacgg ggcccccgtg tctgatggta
aatccacact 7980aggtaccact cttcaacatg gcttcgagct ttatgtccct
actgtgccct atagtgtcat 8040ggagtacctt gattcacgcc cagacacccc
ttttatgtgc actaaacacg gcacttccaa 8100ggctgctgca gaggacctcc
aaaaatacga cctatccacc caaggatttg tcctgcctgg 8160ggtcctacgc
ctagtgcgca ggttcatctt tggccacatt ggcaaggcac cgccattgtt
8220cctcccatca acttatcccg ccaagaactc catggcaggt attaatggtc
agaggttccc 8280aacaaaggat gttcaaagta tacctgaaat tgatgaaatg
tgtgcccgcg ccgtcaagga 8340gaattggcaa actgtgacac cttgcaccct
caagaaacag tattgttcta ggcccaaaac 8400caggaccatc ctgggcacta
acaacttcat agccttggct catagatcgg cgctcagtgg 8460tgttacccag
gcattcatga agaaggcttg gaagtcccca atagccttag ggaaaaacaa
8520attcaaggag ctgcattgca ctgtcgctgg caggtgcctc gaggccgact
tggcttcctg 8580tgaccgcagc acccctgcca ttgtgaggtg gttcactacc
cacctcctat atgaacttgc 8640aggatgtgaa gaatatctac ctagctatgt
gcttaactgt tgccatgacc ttgtggcgac 8700gcaggatggt gctttcacaa
aacgcggtgg cctgtcgtct ggagacccag tcaccagtgt 8760gtccaacact
gtgtactcac tggtgattta tgcccagcac atggtactat ctgccctgaa
8820aatgggtcat gaaattggcc tcaagttcct cgaagaacaa ctcaaatttg
aggaccttct 8880tgaaatccag cctatgttag tatactctga tgatcttgtc
ttgtacgcag aaaagcccac 8940cttccccaac tatcattggt gggtcgagca
tcttgacctg atgttgggct ttaaaacgga 9000cccaaagaaa accgtcataa
ctgataaacc cagtttcctc gggtgcagaa tcgaagcagg 9060gcgacagcta
gtccccaatc gcgaccgcat cctggctgct cttgcatatc acatgaaggc
9120gcagaacgcc tcagagtatt atgcgtctgc tgccgcaatc ctgatggatt
catgtgcttg 9180cattgaccac gatcctgaat ggtatgagga cctcatctgt
ggcattgccc gatgcgctcg 9240cctggacggt tatagctttc caggtccggc
atttttcatg tccatgtggg agaagctgag 9300gagtcataat gaagggaaga
aattccgcca ctgcggcatc tgcgacgcca aagccgacta 9360cgcggctgct
tgtgggcttg atttgtgttt gtttcattcg cactttcacc aacactgccc
9420tgtcactctg agctgcggtc accatgccgg ttcgaaggaa tgttcgcagt
gtcagtcacc 9480tgttggggcc ggcagatccc ctcttgatgc tgtgctggaa
caaattccat acaaacctcc 9540tcgcactgtc atcatgaagg tgggtaataa
aacaacggcc cttgatccgg ggaggtacca 9600gtcccgtcga ggtctcgttg
cagtcaagag gggtattgcg ggtaatgaag ttgatcttgc 9660tgatggagat
taccaagtgg tacctcttct gccgacttgc aaagatataa acatggtgaa
9720ggtggcttgc aacgtactac tcagcaagtt catagtaggg ccaccaggtt
ccgggaagac 9780cacctggcta ctaagtcaag ttcaggacga tgatgtcatt
tacacaccca ctcatcagac 9840catgtttgac atagttagtg ctctcaaagt
ttgcaggtat tccattccag gagcctcggg 9900actccctttc ccaccgcctg
ccaggtccgg gccgtgggtt aggctcattg ccagcgggca 9960cgtccctggc
cgagtatcat acctcgatga ggccggatat tgcaatcatc tggacattct
10020tagactgctt tccaaaacac cccttgtgtg tttgggtgac cttcagcaac
ttcacccagt 10080cggctttgat tcctattgtt atgtgttcga tcagatgcct
cagaagcagt tgaccaccat 10140ttacagattt ggccccaaca tctgcgcagc
catccagcct tgttacaggg agaaacttga 10200atctaaggct aggaacacca
gggtggtttt taccacccgg cctgtggcct ttggccaggt 10260gctgacacca
tatcacaaag atcgcgtcgg ctccgcgatt accatagact catcccaggg
10320ggccaccttt gacattgtaa cattgcatct accatcgcca aagtccctaa
ataagtcccg 10380ggcacttgtg gccatcacac gggcaagaca cgggttgttc
atttatgacc ctcacaacca 10440gctccgggag tttttcaacc taacccctga
gcgcactgat tgtaaccttg tgttcagccg 10500tggagatgag ctggtggtcc
tgaatgcaga taatgcagtc acaaccgtgg cgaaggcctt 10560agagacaggt
ccaactcaat ttcgagtgtc agacccgagg tgcaagtctc tcttagccgc
10620ttgctcggcc agtctggaag ggagctgcat gccgctaccg caagtggcgc
ataacctggg 10680gttttacttc tccccagaca gtccagtatt tgcacctctg
ccaaaagagt tggcgccaca 10740ttggccagtg gttacccatc agaataatcg
ggcgtggcct gatcgacttg tcgctagtat 10800gcgtccaatt gacgcccgct
acagcaagcc gatggtcggt gcagggtatg tggtcggacc 10860gtccaccttc
cttggtactc ctggagtggt gtcatactat ctcacactat acatcagggg
10920tgagccccag gccttgccag aaacacttgt ttcaacagga cgtatagcca
cagattgtcg 10980ggagtatctc gacgcggctg aggaagaggc agcaaaagaa
cttccccacg cgttcattgg 11040cgatgtcaaa ggcaccacag ttggggggtg
tcatcacatt acatcaaaat acctacctag 11100gtccctgcct aaagactctg
ttgccgtagt tggagtaagt tcgcctggca gggctgctaa 11160agccgtatgc
accctcaccg atgtgtacct ccctgaactc cggccatatc tgcaacctga
11220gacggcatca aaatgctgga aactcaaatt agacttcagg gacgtccgac
taatggtctg 11280gaaaggagcc accgcctact ttcaattgga agggctcaca
tggtcggcgc tgcctgacta 11340tgccaggttt attcagctgc ccaaaaacgc
tgttgtatac atcgatccgt gcataggacc 11400ggcaacagcc aatcgtaaag
tcgtacgaac cacagattgg cgggccgacc tggcagtgac 11460gccgtatgat
tacggtgccc ggaacatttt gacaacagcc tggttcgagg acctcgggcc
11520gcagtggaag attctggggt tgcagccctt taggcgggcg tttggctttg
aaaacactga 11580ggattgggca atccttgcat gctgcatgag tgacggcaag
gactacactg actataactg 11640gaattgcgtt cgacaacgcc cacacgctat
ccatggacgc gctcgtgacc atacgtacca 11700ctttgcccct ggcactgaat
tgcaagtgga gctcggtaaa ccccggctgc cacctgagca 11760agtaccgtga
attcggagtg atgcaatggg gtcactgtgg agtaaaatca gccagctgtt
11820cgtggacgcc ttcactgaat tccttgttag tgtggttgat attgtcatct
tccttgccat 11880attgtttggg ttcaccgtcg caggatggtt actggtcttt
cttctcagag tggtttgctc 11940cgcgcttctc cgttcgcgct ctgccattca
ctcttccgaa ctatcgaagg tcctatgagg 12000gcttactacc taattgcaga
ccggatgttc cacaatttgc atttaagcac cctttgggta 12060tgttttggca
catgcgggtt tcccacctaa ttgatcagat ggtctctcgc cgcatctacc
12120agaccatgga acattcaggt caagcggcct ggaagcacgt ggtcagtgag
gctactctta 12180caaaattgtc agaactcgac atagttctcc acttccaaca
cctggccgca gtggaggcgg 12240actcttgtcg cttcctcagc tcacgacttg
tgatgctgaa aaatcttgcc gttggcaatg 12300tgagcttgca gtacaacacc
acgttgaacc gcgttgagct catcctcccc acaccaggta 12360cgaggcccaa
attgaccgat ttcagacaat ggctcatcag tgtgcacgct tccatttttt
12420cctctgtagc ctcatcagtt actttgttca tagtgctttg gcttcgaatt
ccagccgtac 12480gctatgtttt tggtttccat tggcccatgg caacacgtca
ttcgagctga ccattaatta 12540cactatatgc atgccctgtc ttaccagcca
agcggctcaa caaaggctcg aacccggtcg 12600taacatgtgg tgcaaaatag
gacacaccac gtgcgaggag cgtgaccatg atgagttgtc 12660aatgtccatc
ccgtccgggt acgacaacct caaacttgaa ggttattacg cttggctggc
12720ttttttgtcc ttttcctacg cggcccaatt ccatccggag ttgtttggaa
tagggaatgt 12780gtcgcgcgtc tttgtggata aacgacacca gttcatttgt
gccgagcatg acggagataa 12840ttcaaccgta tctaccggac acaacatctc
cgcatcatat gcggcatatt atcaccacca 12900aatagacggg ggcaattggt
tccatttgga atggctgcgg ccgctctttt cctcttggct 12960agtgctcaac
atatcatggt ttctgaggcg ttcgcctgca agccctgttt ctcgacgcat
13020ctatcagata ttaagaccaa tacgaccgcg gctgccggtt tcatggtcct
tcaagacatc 13080agttgcctcc gacctcacag ggtctcagca tcgcaagaga
acattccctt cggaaagtcg 13140tcacaatgtc gtgaagccgt cggtactccc
cagtacatta cgatgactgc taatgtgacc 13200gacgaatcat atttgtacaa
cgcggacttg ctaatgcttt ccgcgtgcct tttccacgcc 13260tcagaaatga
gcgagaaagg cttcaaagtt atctttggaa acgtctccgg cgttgtttca
13320gcttgtgtca atttcacaga ttatgtggcc catgtaaccc aacataccca
acagcatcat 13380ctggtaattg atcacattcg gttactgcat ttcctgacac
catctgcaat gaggtgggct 13440acaaccattg cttgtttgtt cgccattctc
ttagcgatat gagatgttct cacaaattgg 13500ggcgtttctt gactccgcac
tcttgcttct ggtggctttt tttgctgtgt accggcttgt 13560cctggtcctt
tgccgatggc aacggcaaca gctcgacacg ccaatacata tataacttga
13620cgatatgcga gctgaatggg accgtctggt tgtccagtca ttttgattgg
gcagtcgaga 13680cctttgtgct ttacccggtg gccactcata tcctctcact
gggttttctc acaacaagcc 13740atttttttga tgcgctcggt ctcggcgctg
tgtccactac gggatttctt ggcgggcggt 13800atgtacttag cagcgtgtac
ggcgcctgcg ccttcgcagc gcttgtatgt tttgtcatcc 13860gtgctgctaa
aaattgcatg gcttgccgtt atgcccgcac ccggttcacc aacttcatcg
13920tggacgaccg ggggaagatc catcgatgga agtccccaat agtggtagag
aaattaggca 13980aagctgacat cggcggcgac cttgtcacca tcaaacatgt
tgtcctcgaa ggagtcaaag 14040ctcaaccttt gacgaggaca tcggcggagc
aatgggaagc ctagatgatt tttgcaatga 14100tcctaccgcc gcacagaagc
ttgtgctggc atttagtatc acatacacac ctataatgat 14160atacgccctc
aaggtgtcac gcggccggct cctaggactg ttacacatcc tgatatttct
14220gaactgttct ttcacgttcg gatacatgac atacgtgcac tttcaatcca
ctaaccgtgt 14280cgcgcttact atgggggcgg tcgttgccct tttgtggggc
atttacagct ttatagaatc 14340atggaagttt gtcacttcca gatgcaggtt
gtgttgccta ggccggcgat acattctggc 14400ccctgcccac cacgtagaaa
gtgctgcagg cctccattca atcccagcgt ctggtaaccg 14460agcatacgct
gtgagaaagc ccggactaac atcagtgaac ggcactctag taccaggact
14520tcggagcctc gtgttgggcg gcaaacgagc tgttaaacga ggagtggtta
acctcgtcaa 14580gtatggccgg taaaaaccag agccagaaga aaaagaaaaa
cacagctcct atggggagtg 14640gccagccagt caatcaactg tgccaattgc
tgggcacaat gataaagtcc cagcgccagc 14700ggcctagggg aggacaggcc
aaaatgaaaa agcctgagaa gccacatttc cccctagctg 14760ctgaagatga
catccggcac catttcaccc agaccgagcg ttccctttgc ttgcaatcga
14820tccagacggc cttcaatcaa ggcgcaggaa ctgcgtcgct ttcatccagc
gggaaggtca 14880gttttcaggt tgagttcatg ctgccggtcg ctcatacggt
gcgcctgatt cgcgtaactt 14940ccacatccgc cagtcagggt gcaagttaat
ttgatagtta ggtgaatggc cgcgattggc 15000gtgtggcctc tgagtcacct
attcaattag ggcgatcaca tgggggttag acttaattgg 15060cgagaaccat
gtgaccgaaa ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa 151103215107DNAPorcine
reproductive and respiratory syndrome virus 32atgatgtgta gggtattccc
cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc
caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc
gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt
180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg
acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc
cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc
cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct
aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg
tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca
480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat
gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca
agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg
ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct
ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg
gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga
780aaaaatttgt ggtcttcacg gactcctccc ccaacggtat gatgtggacg
ccggaatccg 840atgattcagc cgacctagag gcgctaccgc ctgagctaga
acgtcaggtc gaaatcctca 900ttcggagttt tcctgcccat caccctgtca
gcctggccga ctgggagctc gctgagtccc 960ctgagaacgg tttttccttc
agcacgtacc attctggtgg ttatcttgtc caaaaccccg 1020acgtgtttga
cagcaagtgc tggctctcct gtttcttgga tcagccgatc gaagtgcgcc
1080tctatgagga ttatctggct aacgctttcg gttaccaaac caagtggggc
gtgtctggta 1140agtacctcca gcgcaggctt caagtcaacg gtattcgtgc
tgtaatcgat cctgatggcc 1200ccattcacgt tgaagcgctg tcttgccccc
aatcttggat caggcacctg actctggacg 1260atgacgtcac cccaggattc
gttcgcctaa catccctccg cattgtgccg aacacagagc 1320ccactactct
ccgaatcttt cggtttggag cgcataagtg gtatggcgct gccggcaaac
1380gggctcgtgc caagcgtgcc gctaaaagtg agaagggtcc ggctcccact
cccaaggttg 1440ccccgccagc ccccacctgc ggaattgtta cctactctcc
accaacagac gggtcttgcg 1500gttggcacgt ccttgccgcc ataatgaacc
gaatgatgaa cggtgacttc atgtcccctc 1560tggcccagta caacagacca
gaggatgatt gggcttctga ttacgatctt gcccaggcga 1620ttcaatgtct
gcggttgcct gctaccatag ttcggaatcg tgcctgtccc aacgccaagt
1680acctcataaa actcaacgga gtccactggg aggtagaggt gaggtcaggg
atggcccctc 1740gctcccttcc ccgcgagtgc gtagtcggcg tttgttccga
aggctgtgcc gcatcgcctt 1800acccagaaaa cgggctacct aaacgagcgt
ttgaggcctt ggcgtctgct tacagactac 1860cttccgattg tgtcagttct
ggtattgctg actttcttgc taacccccct caggaattct 1920ggactcttga
caaaatgttg acctccccgt caccggagcg gtctggtttt tccagtctgt
1980acaaattgct attagaggtt gtcccgcaga aatgcggagc cacggaaggg
gctttcacct 2040atgctgttga gagaatgctg aaggattgcc cgagctccga
acaggccatg gcccttctgg 2100caaaaattaa agttccatcc tcaaaggccc
cgtctgtgtc cctggacgag tgtttcccta 2160cggatgtttc agccgatttc
gaaccagcat ctcaggagag gtcccaaaat tccagcgctg 2220ctgttgtcct
gcattcaccg aatgcaaaag agttcgagga agcagctcca ggggaagttc
2280aggagggtgg ccacaaggcc gtccactctg cactccctgc cgggggtcct
aacaataagc 2340aggcacagct ggttgccggt gagcaactga agctcggcgg
ttgtggttcg gtagttggga 2400atgcacatga aggtgttctg gtcccacctg
gtccaattaa tttgacaagc ggggatttac 2460cctcctcagg ctccatgaaa
gaagatatgc tcaatagccg ggaggacgaa ccactggatt 2520tgtcccaacc
agcaacagct gtcacaacga ctcttatggg agagctaaca cccgactacc
2580taggttctga tactggtgcc ctccccgtca ccgtccgaaa atttgtcccg
acggggccta 2640tactccgtca tgttgagcac tgcagcacgg ggtcgggcga
tagcagttcg cctttggatc 2700tgtctggtgc gcaaaccccg gaccagcctt
taaatctgtc cctggcggct tggccagtga 2760ggaccaccgc gtctgatcct
ggctgggtcc acggtagacg cgagcctgtc tttgtaaagc 2820ctcgagatgt
tttctctgat ggcgattcag cccttcagtt cggggagctt tctgaatcca
2880gctctgtcat cgagtttgat cgggcaaaag atgctcaggt ggctgacgcc
cctgtcggtc 2940tgacgacttc ggacgaggcc ctctccgcag tcgatccttt
cgagttttcc gaactcaagc 3000gcccacgttt ctccgcacaa gccttaattg
accgaggcgg cccacttgcc gatgtccatg 3060caaaaataaa gaaccgggta
tatgaacagt gcctccaagc ttgtgagccc ggtagtcgcg 3120caaccccagc
taccagggag tggctcgaca aaatgtggga gagagtggac atgaaaacct
3180ggcgctgcac ctcacagttc caagctggtc acattcttgc gtccctcaaa
ttcctccctg 3240acatgattca agacacaccg cctcctgttc ccaggaagaa
ccgagccagt gataaagctg 3300gcctgaaaca actagtggca cagtgggata
ggagattgag ttcaaccccc cccccaaaac 3360cggttgggcc ggtacttgac
cgggtcgatc ctccgcctac gggtacccgg caagaagacg 3420tcaccccctc
cgatgggcca ccccatgcgc cggatggtcg agtgagtacg ggtgggagtt
3480ggaaaggcct tatgctttcc ggcacccgtc tcacggggtc catcagtcat
cgcctcatga 3540catgggtttt tgaagttgtc tcccatctcc cagcttttat
gctcacactt ttctcgccgc 3600ggggctctat ggctccaggc gattgggttt
ttgcaggtgt tgttttactt gctctcctgc 3660tctgtcgttc ttacccaata
ttcgggtgcc ttcccttatt gggtgtcttt tctggtactg 3720tgcggcgtgt
tcgtctgggt gtttttggct cttggatggc ttttgctgta tttttattct
3780cgactccatc caacccagtc ggttcttctt gtaaccacga ttcgccggag
tgtcatgctg 3840agcttctggc tcttgagcag cgccaacttt gggaacctgt
gcgcggcctt gtggtcggcc 3900catcgggcct cttatgtgtc attcttggca
agttactcgg tgggtcacgt tatctctggc 3960atgttctcct acgtctatgc
ctgcttgcag atttggccct ttctcttgtt tatgtggtgt 4020cccaggggcg
ttgtcacaag tgttggggaa agtgtataag gacagctcct gctgaggtgg
4080cttttaatgt atttcctttc tcgcgcgcca cccgtagttc tcttgtatcc
ttatgtgatc 4140gattccaaac gccaaaagga gttgatcccg tgcacttggc
gacaggttgg cgcgggtgct 4200ggcgtggtga gagtcctatc catcaaccac
accaaaagcc catagcttac gccaatttgg 4260atgaaaagaa gatatctgcc
caaacggttg ttgctgtccc atacgatccc aatcaggcta 4320tcaaatgtct
gaaagttctg caggcgggag gggctatcgt ggatcagcct acgcctgagg
4380tcgttcgtgt gtccgagatc cccttctcag ccccattttt cccaaaagtt
ccagtcaacc 4440cagattgtag ggtcgtggtg gattcggaca cttttgtggc
tgcggttcgt tgtggttatt 4500cgacatcaca actggtcctg ggccagggca
actttgccaa gttaaatcaa acccccccca 4560ggaactctat ctccaccaaa
gcgactggtg gggcctctta tacttttgct gtggctcaag 4620tgtctgtgtg
gacccttgtt catttcgtcc tcggtctttg gctcacgtca cctcaagtgt
4680gtggtcgagg aaccgctgac ccatggtgtt caagtccatt ttcatatcct
acctatggcc 4740ccggggttgt gtgctcctct cgactttgtg tgtctgctga
cggggtcacc cttccattgt 4800tctcagccgt ggcacaactc tctggtaggg
aggtggggat ttttatttta gtgctcgtct 4860cctttattgc cttggcccac
cgcatggctc ttaaggcaga catgttagta gtctttttgg 4920ctctttgtgc
ttatgcctgg cccatgagct cctggttgat ctgcttcttt cctatactct
4980tgaggtgggt tacccttcac cctctcacta tgctttgggt gcattcattc
ttgatgtttt 5040gtctcccagc agccggcgtc ctctcactgg ggataactgg
cctcctctgg gcaatcggcc 5100gctttactca ggttgccgga atcattacac
cttatgatat ccaccagtac acctctgggc 5160cacgtggtgc agccgctgtg
gccacagccc cagaaggcac ttacatggcc gccgtccgga 5220gagctgcttt
aaccgggcgg actttaatct tcaccccgtc cgcagttgga tcccttctcg
5280aaggtgcttt caggactcat aaaccctgcc ttaacaccgt gaatgtcgta
ggctcctccc 5340ttggttccgg aggggttttc accattgacg gaaaaaaaat
tgtcgtcact gctgcccatg 5400tgctgaacgg cgacacagct agagtcaccg
gtgattccta caaccgcatg cacactttca 5460agactaatgg tgactatgcc
tggtcccatg ctgataactg gcagggcgct gcccctgtgg 5520tcaaggttgc
gaaagggtat cgcggtcgtg cctactggca aacatcaact ggtgtcgagc
5580ctggtgttat tgggaatggg ttcgccttct gtttcaccaa ctgcggcgat
tcggggtcac 5640ccgttatctc agaatctggt gatcttatcg gaatccacac
cggttcaaac aaacttggtt 5700ctggtcttgt gacaacccct gaaggggaga
cctgtaccat cagagaaacc aagctttctg 5760acctttccag acatttcgca
ggcccaagcg ttcctcttgg ggacatcaaa ttgagtccgg 5820ccatcatccc
tgatgtgaca tccattccga gtgacttggc atcgctccta gcttccgtcc
5880ctgtaatgga aggcggcctc tcgaccgttc aacttttgtg tgtctttttc
ctcctctggc 5940gcatgatggg ccatgcctgg acgcccattg ttgccgtggg
cttctttttg ctgaatgaaa 6000ttcttccagc agttttggtt cgagccgtgt
tttcttttgc actctttgtg cttgcatggg 6060ccaccccctg gtctgcacaa
gtgttgatga ttagacttct cacggcatct ctcaaccgca 6120acaaactttc
tcttgcgttc tacgcactcg gaggtgttgt tggtttggct gctgaaatcg
6180ggacttttgc tggtaaattg tctgaattgt ctcaagctct ttcgacatac
tgtttcttac 6240ctagggtcct tgctatgacc agctgtgttc ccatcatcat
cattggtgga ctccatgccc 6300tcggtgtgat tctgtggtta ttcaaatacc
ggtgcctcca caacatgctg gttggtgatg 6360gaagcttttc aagcgctttc
ttcctacggt attttgcaga
gggcaatctc aggagaggtg 6420tttcacagtc ctgtggcatg agtaacgagt
ccctgacggc tgctttggct tgcaagttgt 6480cacaggctga ccttgatttt
ttgtccagct taacgaactt caagtgcttt gtatctgctt 6540caaacatgaa
aaatgctgcc ggccagtaca ttgaagcagc ttatgccagg gccctgcgtc
6600aagagttggc ctctttagtc cagattgaca aaatgaaagg agttttgtcc
aagctagagg 6660cctttgctga aacggccact ccgtccctcg acgtaggtga
cgtgattgtt ctacttggac 6720aacatcctca cggatccgtt ctcgatatta
atgtggggac tgaaaggaaa actgtatccg 6780tgcaagagac ccggagccta
ggcggctcca agttcagtgt ttgtactgtt gtgtcaaaca 6840cacccgtgga
cgccttagcc ggtattccac tccagacacc aacccccctt ttcgagaatg
6900gcccgcgtca tcgcagcgag gaggacgatc ttaaagtcga gaggatgaag
aaacactgcg 6960tgtccctcgg cttccacaac attaacggta aagtttactg
caagatttgg gacaagtcta 7020ccggtgacgc cttttacact gatgattccc
ggtacaccca agactatgct tttcaggaca 7080ggtcagctga ctatagagac
agggactacg agggtgtgca aaccgccccc caacagggat 7140ttgatccaaa
gtctgaaacc cctgttggta ccgttgtgat cggcggtatt acgtacaaca
7200ggtatttggt caaaggtaag gaggttctgg ttcccaagcc tgacaactgc
cttgaagctg 7260ccaagctgtc ccttgagcaa gctctcgctg ggatgggcca
aacttgtgac cttacagctg 7320ccgaggtgga aaagctaaag cgcatcattg
gtcaacttca aggattgacc actgagcagg 7380ctttaaactg ttagccgcca
gcggcttgac ccgctgtggc cgcggcggcc tagttgtaac 7440tgaaacggcg
gtaaaaattg tcaaatacca cagcagaact tttaccttag gctctttaga
7500cctaaaagtc acttccgagg tggaggtgaa gaagtcaacc gagcagggcc
acgctgttgt 7560ggcaaacttg tgttctggtg tcgtcttgat gagacctcac
ccaccgtccc ttgtcgacgt 7620tcttctgaaa cccggacttg acataacacc
cggcattcaa ccagggcatg gggccgggaa 7680tatgggcgtg gacggttcca
tttgggattt tgaaaccgca cccacaaagg ctgaactcga 7740gttatccaag
caaataattc aagcatgtga agtcaggcgc ggggatgccc cgaacctcca
7800gctcccttac aagctctatc ctgttagagg ggatcctgag cggcataaag
gccacctcat 7860caataccagg tttggagact taccttacaa aactcctcaa
gacaccaagt ccgcaatcca 7920cgcggcttgt tgcctgcacc ccaacggggc
ccccgtgtct gatggtaaat ccacactagg 7980taccactctt caacatggct
tcgagcttta tgtccctact gtgccctata gtgtcatgga 8040gtaccttgat
tcacgcccag acaccccttt tatgtgcact aaacacggca cttccaaggc
8100tgctgcagag gacctccaaa aatacgacct atccacccaa ggatttgtcc
tgcctggggt 8160cctacgccta gtgcgcaggt tcatctttgg ccacattggc
aaggcaccgc cattgttcct 8220cccatcaact tatcccgcca agaactccat
ggcaggtatt aatggtcaga ggttcccaac 8280aaaggatgtt caaagtatac
ctgaaattga tgaaatgtgt gcccgcgccg tcaaggagaa 8340ttggcaaact
gtgacacctt gcaccctcaa gaaacagtat tgttctaggc ccaaaaccag
8400gaccatcctg ggcactaaca acttcatagc cttggctcat agatcggcgc
tcagtggtgt 8460tacccaggca ttcatgaaga aggcttggaa gtccccaata
gccttaggga aaaacaaatt 8520caaggagctg cattgcactg tcgctggcag
gtgcctcgag gccgacttgg cttcctgtga 8580ccgcagcacc cctgccattg
tgaggtggtt cactacccac ctcctatatg aacttgcagg 8640atgtgaagaa
tatctaccta gctatgtgct taactgttgc catgaccttg tggcgacgca
8700ggatggtgct ttcacaaaac gcggtggcct gtcgtctgga gacccagtca
ccagtgtgtc 8760caacactgtg tactcactgg tgatttatgc ccagcacatg
gtactatctg ccctgaaaat 8820gggtcatgaa attggcctca agttcctcga
agaacaactc aaatttgagg accttcttga 8880aatccagcct atgttagtat
actctgatga tcttgtcttg tacgcagaaa agcccacctt 8940ccccaactat
cattggtggg tcgagcatct tgacctgatg ttgggcttta aaacggaccc
9000aaagaaaacc gtcataactg ataaacccag tttcctcggg tgcagaatcg
aagcagggcg 9060acagctagtc cccaatcgcg accgcatcct ggctgctctt
gcatatcaca tgaaggcgca 9120gaacgcctca gagtattatg cgtctgctgc
cgcaatcctg atggattcat gtgcttgcat 9180tgaccacgat cctgaatggt
atgaggacct catctgtggc attgcccgat gcgctcgcct 9240ggacggttat
agctttccag gtccggcatt tttcatgtcc atgtgggaga agctgaggag
9300tcataatgaa gggaagaaat tccgccactg cggcatctgc gacgccaaag
ccgactacgc 9360ggctgcttgt gggcttgatt tgtgtttgtt tcattcgcac
tttcaccaac actgccctgt 9420cactctgagc tgcggtcacc atgccggttc
gaaggaatgt tcgcagtgtc agtcacctgt 9480tggggccggc agatcccctc
ttgatgctgt gctggaacaa attccataca aacctcctcg 9540cactgtcatc
atgaaggtgg gtaataaaac aacggccctt gatccgggga ggtaccagtc
9600ccgtcgaggt ctcgttgcag tcaagagggg tattgcgggt aatgaagttg
atcttgctga 9660tggagattac caagtggtac ctcttctgcc gacttgcaaa
gatataaaca tggtgaaggt 9720ggcttgcaac gtactactca gcaagttcat
agtagggcca ccaggttccg ggaagaccac 9780ctggctacta agtcaagttc
aggacgatga tgtcatttac acacccactc atcagaccat 9840gtttgacata
gttagtgctc tcaaagtttg caggtattcc attccaggag cctcgggact
9900ccctttccca ccgcctgcca ggtccgggcc gtgggttagg ctcattgcca
gcgggcacgt 9960ccctggccga gtatcatacc tcgatgaggc cggatattgc
aatcatctgg acattcttag 10020actgctttcc aaaacacccc ttgtgtgttt
gggtgacctt cagcaacttc acccagtcgg 10080ctttgattcc tattgttatg
tgttcgatca gatgcctcag aagcagttga ccaccattta 10140cagatttggc
cccaacatct gcgcagccat ccagccttgt tacagggaga aacttgaatc
10200taaggctagg aacaccaggg tggtttttac cacccggcct gtggcctttg
gccaggtgct 10260gacaccatat cacaaagatc gcgtcggctc cgcgattacc
atagactcat cccagggggc 10320cacctttgac attgtaacat tgcatctacc
atcgccaaag tccctaaata agtcccgggc 10380acttgtggcc atcacacggg
caagacacgg gttgttcatt tatgaccctc acaaccagct 10440ccgggagttt
ttcaacctaa cccctgagcg cactgattgt aaccttgtgt tcagccgtgg
10500agatgagctg gtggtcctga atgcagataa tgcagtcaca accgtggcga
aggccttaga 10560gacaggtcca actcaatttc gagtgtcaga cccgaggtgc
aagtctctct tagccgcttg 10620ctcggccagt ctggaaggga gctgcatgcc
gctaccgcaa gtggcgcata acctggggtt 10680ttacttctcc ccagacagtc
cagtatttgc acctctgcca aaagagttgg cgccacattg 10740gccagtggtt
acccatcaga ataatcgggc gtggcctgat cgacttgtcg ctagtatgcg
10800tccaattgac gcccgctaca gcaagccgat ggtcggtgca gggtatgtgg
tcggaccgtc 10860caccttcctt ggtactcctg gagtggtgtc atactatctc
acactataca tcaggggtga 10920gccccaggcc ttgccagaaa cacttgtttc
aacaggacgt atagccacag attgtcggga 10980gtatctcgac gcggctgagg
aagaggcagc aaaagaactt ccccacgcgt tcattggcga 11040tgtcaaaggc
accacagttg gggggtgtca tcacattaca tcaaaatacc tacctaggtc
11100cctgcctaaa gactctgttg ccgtagttgg agtaagttcg cctggcaggg
ctgctaaagc 11160cgtatgcacc ctcaccgatg tgtacctccc tgaactccgg
ccatatctgc aacctgagac 11220ggcatcaaaa tgctggaaac tcaaattaga
cttcagggac gtccgactaa tggtctggaa 11280aggagccacc gcctactttc
aattggaagg gctcacatgg tcggcgctgc ctgactatgc 11340caggtttatt
cagctgccca aaaacgctgt tgtatacatc gatccgtgca taggaccggc
11400aacagccaat cgtaaagtcg tacgaaccac agattggcgg gccgacctgg
cagtgacgcc 11460gtatgattac ggtgcccgga acattttgac aacagcctgg
ttcgaggacc tcgggccgca 11520gtggaagatt ctggggttgc agccctttag
gcgggcgttt ggctttgaaa acactgagga 11580ttgggcaatc cttgcatgct
gcatgagtga cggcaaggac tacactgact ataactggaa 11640ttgcgttcga
caacgcccac acgctatcca tggacgcgct cgtgaccata cgtaccactt
11700tgcccctggc actgaattgc aagtggagct cggtaaaccc cggctgccac
ctgagcaagt 11760accgtgaatt cggagtgatg caatggggtc actgtggagt
aaaatcagcc agctgttcgt 11820ggacgccttc actgaattcc ttgttagtgt
ggttgatatt gtcatcttcc ttgccatatt 11880gtttgggttc accgtcgcag
gatggttact ggtctttctt ctcagagtgg tttgctccgc 11940gcttctccgt
tcgcgctctg ccattcactc ttccgaacta tcgaaggtcc tatgagggct
12000tactacctaa ttgcagaccg gatgttccac aatttgcatt taagcaccct
ttgggtatgt 12060tttggcacat gcgggtttcc cacctaattg atcagatggt
ctctcgccgc atctaccaga 12120ccatggaaca ttcaggtcaa gcggcctgga
agcacgtggt cagtgaggct actcttacaa 12180aattgtcaga actcgacata
gttctccact tccaacacct ggccgcagtg gaggcggact 12240cttgtcgctt
cctcagctca cgacttgtga tgctgaaaaa tcttgccgtt ggcaatgtga
12300gcttgcagta caacaccacg ttgaaccgcg ttgagctcat cctccccaca
ccaggtacga 12360ggcccaaatt gaccgatttc agacaatggc tcatcagtgt
gcacgcttcc attttttcct 12420ctgtagcctc atcagttact ttgttcatag
tgctttggct tcgaattcca gccgtacgct 12480atgtttttgg tttccattgg
cccatggcaa cacgtcattc gagctgacca ttaattacac 12540tatatgcatg
ccctgtctta ccagccaagc ggctcaacaa aggctcgaac ccggtcgtaa
12600catgtggtgc aaaataggac acaccacgtg cgaggagcgt gaccatgatg
agttgtcaat 12660gtccatcccg tccgggtacg acaacctcaa acttgaaggt
tattacgctt ggctggcttt 12720tttgtccttt tcctacgcgg cccaattcca
tccggagttg tttggaatag ggaatgtgtc 12780gcgcgtcttt gtggataaac
gacaccagtt catttgtgcc gagcatgacg gagataattc 12840aaccgtatct
accggacaca acatctccgc atcatatgcg gcatattatc accaccaaat
12900agacgggggc aattggttcc atttggaatg gctgcggccg ctcttttcct
cttggctagt 12960gctcaacata tcatggtttc tgaggcgttc gcctgcaagc
cctgtttctc gacgcatcta 13020tcagatatta agaccaatac gaccgcggct
gccggtttca tggtccttca agacatcagt 13080tgcctccgac ctcacagggt
ctcagcatcg caagagaaca ttcccttcgg aaagtcgtca 13140caatgtcgtg
aagccgtcgg tactccccag tacattacga tgactgctaa tgtgaccgac
13200gaatcatatt tgtacaacgc ggacttgcta atgctttccg cgtgcctttt
ccacgcctca 13260gaaatgagcg agaaaggctt caaagttatc tttggaaacg
tctccggcgt tgtttcagct 13320tgtgtcaatt tcacagatta tgtggcccat
gtaacccaac atacccaaca gcatcatctg 13380gtaattgatc acattcggtt
actgcatttc ctgacaccat ctgcaatgag gtgggctaca 13440accattgctt
gtttgttcgc cattctctta gcgatatgag atgttctcac aaattggggc
13500gtttcttgac tccgcactct tgcttctggt ggcttttttt gctgtgtacc
ggcttgtcct 13560ggtcctttgc cgatggcaac ggcaacagct cgacacgcca
atacatatat aacttgacga 13620tatgcgagct gaatgggacc gtctggttgt
ccagtcattt tgattgggca gtcgagacct 13680ttgtgcttta cccggtggcc
actcatatcc tctcactggg ttttctcaca acaagccatt 13740tttttgatgc
gctcggtctc ggcgctgtgt ccactacggg atttcttggc gggcggtatg
13800tacttagcag cgtgtacggc gcctgcgcct tcgcagcgct tgtatgtttt
gtcatccgtg 13860ctgctaaaaa ttgcatggct tgccgttatg cccgcacccg
gttcaccaac ttcatcgtgg 13920acgaccgggg gaagatccat cgatggaagt
ccccaatagt ggtagagaaa ttaggcaaag 13980ctgacatcgg cggcgacctt
gtcaccatca aacatgttgt cctcgaagga gtcaaagctc 14040aacctttgac
gaggacatcg gcggagcaat gggaagccta gatgattttt gcaatgatcc
14100taccgccgca cagaagcttg tgctggcatt tagtatcaca tacacaccta
taatgatata 14160cgccctcaag gtgtcacgcg gccggctcct aggactgtta
cacatcctga tatttctgaa 14220ctgttctttc acgttcggat acatgacata
cgtgcacttt caatccacta accgtgtcgc 14280gcttactatg ggggcggtcg
ttgccctttt gtggggcatt tacagcttta tagaatcatg 14340gaagtttgtc
acttccagat gcaggttgtg ttgcctaggc cggcgataca ttctggcccc
14400tgcccaccac gtagaaagtg ctgcaggcct ccattcaatc ccagcgtctg
gtaaccgagc 14460atacgctgtg agaaagcccg gactaacatc agtgaacggc
actctagtac caggacttcg 14520gagcctcgtg ttgggcggca aacgagctgt
taaacgagga gtggttaacc tcgtcaagta 14580tggccggtaa aaaccagagc
cagaagaaaa agaaaaacac agctcctatg gggagtggcc 14640agccagtcaa
tcaactgtgc caattgctgg gcacaatgat aaagtcccag cgccagcggc
14700ctaggggagg acaggccaaa atgaaaaagc ctgagaagcc acatttcccc
ctagctgctg 14760aagatgacat ccggcaccat ttcacccaga ccgagcgttc
cctttgcttg caatcgatcc 14820agacggcctt caatcaaggc gcaggaactg
cgtcgctttc atccagcggg aaggtcagtt 14880ttcaggttga gttcatgctg
ccggtcgctc atacggtgcg cctgattcgc gtaacttcca 14940catccgccag
tcagggtgca agttaatttg atagttaggt gaatggccgc gattggcgtg
15000tggcctctga gtcacctatt caattagggc gatcacatgg gggttagact
taattggcga 15060gaaccatgtg accgaaatta aaaaaaaaaa aaaaaaaaaa aaaaaaa
151073315104DNAPorcine reproductive and respiratory syndrome virus
33atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag
60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct
120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct
cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat
tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct
cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt
ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc
420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc
ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc
gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg
ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca
gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac
720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg
tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacatgat
gtggacgccg gaatccgatg 840attcagccga cctagaggcg ctaccgcctg
agctagaacg tcaggtcgaa atcctcattc 900ggagttttcc tgcccatcac
cctgtcagcc tggccgactg ggagctcgct gagtcccctg 960agaacggttt
ttccttcagc acgtaccatt ctggtggtta tcttgtccaa aaccccgacg
1020tgtttgacag caagtgctgg ctctcctgtt tcttggatca gccgatcgaa
gtgcgcctct 1080atgaggatta tctggctaac gctttcggtt accaaaccaa
gtggggcgtg tctggtaagt 1140acctccagcg caggcttcaa gtcaacggta
ttcgtgctgt aatcgatcct gatggcccca 1200ttcacgttga agcgctgtct
tgcccccaat cttggatcag gcacctgact ctggacgatg 1260acgtcacccc
aggattcgtt cgcctaacat ccctccgcat tgtgccgaac acagagccca
1320ctactctccg aatctttcgg tttggagcgc ataagtggta tggcgctgcc
ggcaaacggg 1380ctcgtgccaa gcgtgccgct aaaagtgaga agggtccggc
tcccactccc aaggttgccc 1440cgccagcccc cacctgcgga attgttacct
actctccacc aacagacggg tcttgcggtt 1500ggcacgtcct tgccgccata
atgaaccgaa tgatgaacgg tgacttcatg tcccctctgg 1560cccagtacaa
cagaccagag gatgattggg cttctgatta cgatcttgcc caggcgattc
1620aatgtctgcg gttgcctgct accatagttc ggaatcgtgc ctgtcccaac
gccaagtacc 1680tcataaaact caacggagtc cactgggagg tagaggtgag
gtcagggatg gcccctcgct 1740cccttccccg cgagtgcgta gtcggcgttt
gttccgaagg ctgtgccgca tcgccttacc 1800cagaaaacgg gctacctaaa
cgagcgtttg aggccttggc gtctgcttac agactacctt 1860ccgattgtgt
cagttctggt attgctgact ttcttgctaa cccccctcag gaattctgga
1920ctcttgacaa aatgttgacc tccccgtcac cggagcggtc tggtttttcc
agtctgtaca 1980aattgctatt agaggttgtc ccgcagaaat gcggagccac
ggaaggggct ttcacctatg 2040ctgttgagag aatgctgaag gattgcccga
gctccgaaca ggccatggcc cttctggcaa 2100aaattaaagt tccatcctca
aaggccccgt ctgtgtccct ggacgagtgt ttccctacgg 2160atgtttcagc
cgatttcgaa ccagcatctc aggagaggtc ccaaaattcc agcgctgctg
2220ttgtcctgca ttcaccgaat gcaaaagagt tcgaggaagc agctccaggg
gaagttcagg 2280agggtggcca caaggccgtc cactctgcac tccctgccgg
gggtcctaac aataagcagg 2340cacagctggt tgccggtgag caactgaagc
tcggcggttg tggttcggta gttgggaatg 2400cacatgaagg tgttctggtc
ccacctggtc caattaattt gacaagcggg gatttaccct 2460cctcaggctc
catgaaagaa gatatgctca atagccggga ggacgaacca ctggatttgt
2520cccaaccagc aacagctgtc acaacgactc ttatgggaga gctaacaccc
gactacctag 2580gttctgatac tggtgccctc cccgtcaccg tccgaaaatt
tgtcccgacg gggcctatac 2640tccgtcatgt tgagcactgc agcacggggt
cgggcgatag cagttcgcct ttggatctgt 2700ctggtgcgca aaccccggac
cagcctttaa atctgtccct ggcggcttgg ccagtgagga 2760ccaccgcgtc
tgatcctggc tgggtccacg gtagacgcga gcctgtcttt gtaaagcctc
2820gagatgtttt ctctgatggc gattcagccc ttcagttcgg ggagctttct
gaatccagct 2880ctgtcatcga gtttgatcgg gcaaaagatg ctcaggtggc
tgacgcccct gtcggtctga 2940cgacttcgga cgaggccctc tccgcagtcg
atcctttcga gttttccgaa ctcaagcgcc 3000cacgtttctc cgcacaagcc
ttaattgacc gaggcggccc acttgccgat gtccatgcaa 3060aaataaagaa
ccgggtatat gaacagtgcc tccaagcttg tgagcccggt agtcgcgcaa
3120ccccagctac cagggagtgg ctcgacaaaa tgtgggagag agtggacatg
aaaacctggc 3180gctgcacctc acagttccaa gctggtcaca ttcttgcgtc
cctcaaattc ctccctgaca 3240tgattcaaga cacaccgcct cctgttccca
ggaagaaccg agccagtgat aaagctggcc 3300tgaaacaact agtggcacag
tgggatagga gattgagttc aacccccccc ccaaaaccgg 3360ttgggccggt
acttgaccgg gtcgatcctc cgcctacggg tacccggcaa gaagacgtca
3420ccccctccga tgggccaccc catgcgccgg atggtcgagt gagtacgggt
gggagttgga 3480aaggccttat gctttccggc acccgtctca cggggtccat
cagtcatcgc ctcatgacat 3540gggtttttga agttgtctcc catctcccag
cttttatgct cacacttttc tcgccgcggg 3600gctctatggc tccaggcgat
tgggtttttg caggtgttgt tttacttgct ctcctgctct 3660gtcgttctta
cccaatattc gggtgccttc ccttattggg tgtcttttct ggtactgtgc
3720ggcgtgttcg tctgggtgtt tttggctctt ggatggcttt tgctgtattt
ttattctcga 3780ctccatccaa cccagtcggt tcttcttgta accacgattc
gccggagtgt catgctgagc 3840ttctggctct tgagcagcgc caactttggg
aacctgtgcg cggccttgtg gtcggcccat 3900cgggcctctt atgtgtcatt
cttggcaagt tactcggtgg gtcacgttat ctctggcatg 3960ttctcctacg
tctatgcctg cttgcagatt tggccctttc tcttgtttat gtggtgtccc
4020aggggcgttg tcacaagtgt tggggaaagt gtataaggac agctcctgct
gaggtggctt 4080ttaatgtatt tcctttctcg cgcgccaccc gtagttctct
tgtatcctta tgtgatcgat 4140tccaaacgcc aaaaggagtt gatcccgtgc
acttggcgac aggttggcgc gggtgctggc 4200gtggtgagag tcctatccat
caaccacacc aaaagcccat agcttacgcc aatttggatg 4260aaaagaagat
atctgcccaa acggttgttg ctgtcccata cgatcccaat caggctatca
4320aatgtctgaa agttctgcag gcgggagggg ctatcgtgga tcagcctacg
cctgaggtcg 4380ttcgtgtgtc cgagatcccc ttctcagccc catttttccc
aaaagttcca gtcaacccag 4440attgtagggt cgtggtggat tcggacactt
ttgtggctgc ggttcgttgt ggttattcga 4500catcacaact ggtcctgggc
cagggcaact ttgccaagtt aaatcaaacc ccccccagga 4560actctatctc
caccaaagcg actggtgggg cctcttatac ttttgctgtg gctcaagtgt
4620ctgtgtggac ccttgttcat ttcgtcctcg gtctttggct cacgtcacct
caagtgtgtg 4680gtcgaggaac cgctgaccca tggtgttcaa gtccattttc
atatcctacc tatggccccg 4740gggttgtgtg ctcctctcga ctttgtgtgt
ctgctgacgg ggtcaccctt ccattgttct 4800cagccgtggc acaactctct
ggtagggagg tggggatttt tattttagtg ctcgtctcct 4860ttattgcctt
ggcccaccgc atggctctta aggcagacat gttagtagtc tttttggctc
4920tttgtgctta tgcctggccc atgagctcct ggttgatctg cttctttcct
atactcttga 4980ggtgggttac ccttcaccct ctcactatgc tttgggtgca
ttcattcttg atgttttgtc 5040tcccagcagc cggcgtcctc tcactgggga
taactggcct cctctgggca atcggccgct 5100ttactcaggt tgccggaatc
attacacctt atgatatcca ccagtacacc tctgggccac 5160gtggtgcagc
cgctgtggcc acagccccag aaggcactta catggccgcc gtccggagag
5220ctgctttaac cgggcggact ttaatcttca ccccgtccgc agttggatcc
cttctcgaag 5280gtgctttcag gactcataaa ccctgcctta acaccgtgaa
tgtcgtaggc tcctcccttg 5340gttccggagg ggttttcacc attgacggaa
aaaaaattgt cgtcactgct gcccatgtgc 5400tgaacggcga cacagctaga
gtcaccggtg attcctacaa ccgcatgcac actttcaaga 5460ctaatggtga
ctatgcctgg tcccatgctg ataactggca gggcgctgcc cctgtggtca
5520aggttgcgaa agggtatcgc ggtcgtgcct actggcaaac atcaactggt
gtcgagcctg 5580gtgttattgg gaatgggttc gccttctgtt tcaccaactg
cggcgattcg gggtcacccg 5640ttatctcaga atctggtgat cttatcggaa
tccacaccgg ttcaaacaaa cttggttctg 5700gtcttgtgac aacccctgaa
ggggagacct gtaccatcag agaaaccaag ctttctgacc 5760tttccagaca
tttcgcaggc ccaagcgttc ctcttgggga catcaaattg agtccggcca
5820tcatccctga tgtgacatcc attccgagtg acttggcatc gctcctagct
tccgtccctg 5880taatggaagg cggcctctcg accgttcaac ttttgtgtgt
ctttttcctc ctctggcgca 5940tgatgggcca tgcctggacg cccattgttg
ccgtgggctt ctttttgctg aatgaaattc 6000ttccagcagt tttggttcga
gccgtgtttt cttttgcact ctttgtgctt gcatgggcca 6060ccccctggtc
tgcacaagtg ttgatgatta gacttctcac ggcatctctc aaccgcaaca
6120aactttctct tgcgttctac gcactcggag gtgttgttgg tttggctgct
gaaatcggga 6180cttttgctgg taaattgtct gaattgtctc aagctctttc
gacatactgt ttcttaccta 6240gggtccttgc tatgaccagc tgtgttccca
tcatcatcat
tggtggactc catgccctcg 6300gtgtgattct gtggttattc aaataccggt
gcctccacaa catgctggtt ggtgatggaa 6360gcttttcaag cgctttcttc
ctacggtatt ttgcagaggg caatctcagg agaggtgttt 6420cacagtcctg
tggcatgagt aacgagtccc tgacggctgc tttggcttgc aagttgtcac
6480aggctgacct tgattttttg tccagcttaa cgaacttcaa gtgctttgta
tctgcttcaa 6540acatgaaaaa tgctgccggc cagtacattg aagcagctta
tgccagggcc ctgcgtcaag 6600agttggcctc tttagtccag attgacaaaa
tgaaaggagt tttgtccaag ctagaggcct 6660ttgctgaaac ggccactccg
tccctcgacg taggtgacgt gattgttcta cttggacaac 6720atcctcacgg
atccgttctc gatattaatg tggggactga aaggaaaact gtatccgtgc
6780aagagacccg gagcctaggc ggctccaagt tcagtgtttg tactgttgtg
tcaaacacac 6840ccgtggacgc cttagccggt attccactcc agacaccaac
cccccttttc gagaatggcc 6900cgcgtcatcg cagcgaggag gacgatctta
aagtcgagag gatgaagaaa cactgcgtgt 6960ccctcggctt ccacaacatt
aacggtaaag tttactgcaa gatttgggac aagtctaccg 7020gtgacgcctt
ttacactgat gattcccggt acacccaaga ctatgctttt caggacaggt
7080cagctgacta tagagacagg gactacgagg gtgtgcaaac cgccccccaa
cagggatttg 7140atccaaagtc tgaaacccct gttggtaccg ttgtgatcgg
cggtattacg tacaacaggt 7200atttggtcaa aggtaaggag gttctggttc
ccaagcctga caactgcctt gaagctgcca 7260agctgtccct tgagcaagct
ctcgctggga tgggccaaac ttgtgacctt acagctgccg 7320aggtggaaaa
gctaaagcgc atcattggtc aacttcaagg attgaccact gagcaggctt
7380taaactgtta gccgccagcg gcttgacccg ctgtggccgc ggcggcctag
ttgtaactga 7440aacggcggta aaaattgtca aataccacag cagaactttt
accttaggct ctttagacct 7500aaaagtcact tccgaggtgg aggtgaagaa
gtcaaccgag cagggccacg ctgttgtggc 7560aaacttgtgt tctggtgtcg
tcttgatgag acctcaccca ccgtcccttg tcgacgttct 7620tctgaaaccc
ggacttgaca taacacccgg cattcaacca gggcatgggg ccgggaatat
7680gggcgtggac ggttccattt gggattttga aaccgcaccc acaaaggctg
aactcgagtt 7740atccaagcaa ataattcaag catgtgaagt caggcgcggg
gatgccccga acctccagct 7800cccttacaag ctctatcctg ttagagggga
tcctgagcgg cataaaggcc acctcatcaa 7860taccaggttt ggagacttac
cttacaaaac tcctcaagac accaagtccg caatccacgc 7920ggcttgttgc
ctgcacccca acggggcccc cgtgtctgat ggtaaatcca cactaggtac
7980cactcttcaa catggcttcg agctttatgt ccctactgtg ccctatagtg
tcatggagta 8040ccttgattca cgcccagaca ccccttttat gtgcactaaa
cacggcactt ccaaggctgc 8100tgcagaggac ctccaaaaat acgacctatc
cacccaagga tttgtcctgc ctggggtcct 8160acgcctagtg cgcaggttca
tctttggcca cattggcaag gcaccgccat tgttcctccc 8220atcaacttat
cccgccaaga actccatggc aggtattaat ggtcagaggt tcccaacaaa
8280ggatgttcaa agtatacctg aaattgatga aatgtgtgcc cgcgccgtca
aggagaattg 8340gcaaactgtg acaccttgca ccctcaagaa acagtattgt
tctaggccca aaaccaggac 8400catcctgggc actaacaact tcatagcctt
ggctcataga tcggcgctca gtggtgttac 8460ccaggcattc atgaagaagg
cttggaagtc cccaatagcc ttagggaaaa acaaattcaa 8520ggagctgcat
tgcactgtcg ctggcaggtg cctcgaggcc gacttggctt cctgtgaccg
8580cagcacccct gccattgtga ggtggttcac tacccacctc ctatatgaac
ttgcaggatg 8640tgaagaatat ctacctagct atgtgcttaa ctgttgccat
gaccttgtgg cgacgcagga 8700tggtgctttc acaaaacgcg gtggcctgtc
gtctggagac ccagtcacca gtgtgtccaa 8760cactgtgtac tcactggtga
tttatgccca gcacatggta ctatctgccc tgaaaatggg 8820tcatgaaatt
ggcctcaagt tcctcgaaga acaactcaaa tttgaggacc ttcttgaaat
8880ccagcctatg ttagtatact ctgatgatct tgtcttgtac gcagaaaagc
ccaccttccc 8940caactatcat tggtgggtcg agcatcttga cctgatgttg
ggctttaaaa cggacccaaa 9000gaaaaccgtc ataactgata aacccagttt
cctcgggtgc agaatcgaag cagggcgaca 9060gctagtcccc aatcgcgacc
gcatcctggc tgctcttgca tatcacatga aggcgcagaa 9120cgcctcagag
tattatgcgt ctgctgccgc aatcctgatg gattcatgtg cttgcattga
9180ccacgatcct gaatggtatg aggacctcat ctgtggcatt gcccgatgcg
ctcgcctgga 9240cggttatagc tttccaggtc cggcattttt catgtccatg
tgggagaagc tgaggagtca 9300taatgaaggg aagaaattcc gccactgcgg
catctgcgac gccaaagccg actacgcggc 9360tgcttgtggg cttgatttgt
gtttgtttca ttcgcacttt caccaacact gccctgtcac 9420tctgagctgc
ggtcaccatg ccggttcgaa ggaatgttcg cagtgtcagt cacctgttgg
9480ggccggcaga tcccctcttg atgctgtgct ggaacaaatt ccatacaaac
ctcctcgcac 9540tgtcatcatg aaggtgggta ataaaacaac ggcccttgat
ccggggaggt accagtcccg 9600tcgaggtctc gttgcagtca agaggggtat
tgcgggtaat gaagttgatc ttgctgatgg 9660agattaccaa gtggtacctc
ttctgccgac ttgcaaagat ataaacatgg tgaaggtggc 9720ttgcaacgta
ctactcagca agttcatagt agggccacca ggttccggga agaccacctg
9780gctactaagt caagttcagg acgatgatgt catttacaca cccactcatc
agaccatgtt 9840tgacatagtt agtgctctca aagtttgcag gtattccatt
ccaggagcct cgggactccc 9900tttcccaccg cctgccaggt ccgggccgtg
ggttaggctc attgccagcg ggcacgtccc 9960tggccgagta tcatacctcg
atgaggccgg atattgcaat catctggaca ttcttagact 10020gctttccaaa
acaccccttg tgtgtttggg tgaccttcag caacttcacc cagtcggctt
10080tgattcctat tgttatgtgt tcgatcagat gcctcagaag cagttgacca
ccatttacag 10140atttggcccc aacatctgcg cagccatcca gccttgttac
agggagaaac ttgaatctaa 10200ggctaggaac accagggtgg tttttaccac
ccggcctgtg gcctttggcc aggtgctgac 10260accatatcac aaagatcgcg
tcggctccgc gattaccata gactcatccc agggggccac 10320ctttgacatt
gtaacattgc atctaccatc gccaaagtcc ctaaataagt cccgggcact
10380tgtggccatc acacgggcaa gacacgggtt gttcatttat gaccctcaca
accagctccg 10440ggagtttttc aacctaaccc ctgagcgcac tgattgtaac
cttgtgttca gccgtggaga 10500tgagctggtg gtcctgaatg cagataatgc
agtcacaacc gtggcgaagg ccttagagac 10560aggtccaact caatttcgag
tgtcagaccc gaggtgcaag tctctcttag ccgcttgctc 10620ggccagtctg
gaagggagct gcatgccgct accgcaagtg gcgcataacc tggggtttta
10680cttctcccca gacagtccag tatttgcacc tctgccaaaa gagttggcgc
cacattggcc 10740agtggttacc catcagaata atcgggcgtg gcctgatcga
cttgtcgcta gtatgcgtcc 10800aattgacgcc cgctacagca agccgatggt
cggtgcaggg tatgtggtcg gaccgtccac 10860cttccttggt actcctggag
tggtgtcata ctatctcaca ctatacatca ggggtgagcc 10920ccaggccttg
ccagaaacac ttgtttcaac aggacgtata gccacagatt gtcgggagta
10980tctcgacgcg gctgaggaag aggcagcaaa agaacttccc cacgcgttca
ttggcgatgt 11040caaaggcacc acagttgggg ggtgtcatca cattacatca
aaatacctac ctaggtccct 11100gcctaaagac tctgttgccg tagttggagt
aagttcgcct ggcagggctg ctaaagccgt 11160atgcaccctc accgatgtgt
acctccctga actccggcca tatctgcaac ctgagacggc 11220atcaaaatgc
tggaaactca aattagactt cagggacgtc cgactaatgg tctggaaagg
11280agccaccgcc tactttcaat tggaagggct cacatggtcg gcgctgcctg
actatgccag 11340gtttattcag ctgcccaaaa acgctgttgt atacatcgat
ccgtgcatag gaccggcaac 11400agccaatcgt aaagtcgtac gaaccacaga
ttggcgggcc gacctggcag tgacgccgta 11460tgattacggt gcccggaaca
ttttgacaac agcctggttc gaggacctcg ggccgcagtg 11520gaagattctg
gggttgcagc cctttaggcg ggcgtttggc tttgaaaaca ctgaggattg
11580ggcaatcctt gcatgctgca tgagtgacgg caaggactac actgactata
actggaattg 11640cgttcgacaa cgcccacacg ctatccatgg acgcgctcgt
gaccatacgt accactttgc 11700ccctggcact gaattgcaag tggagctcgg
taaaccccgg ctgccacctg agcaagtacc 11760gtgaattcgg agtgatgcaa
tggggtcact gtggagtaaa atcagccagc tgttcgtgga 11820cgccttcact
gaattccttg ttagtgtggt tgatattgtc atcttccttg ccatattgtt
11880tgggttcacc gtcgcaggat ggttactggt ctttcttctc agagtggttt
gctccgcgct 11940tctccgttcg cgctctgcca ttcactcttc cgaactatcg
aaggtcctat gagggcttac 12000tacctaattg cagaccggat gttccacaat
ttgcatttaa gcaccctttg ggtatgtttt 12060ggcacatgcg ggtttcccac
ctaattgatc agatggtctc tcgccgcatc taccagacca 12120tggaacattc
aggtcaagcg gcctggaagc acgtggtcag tgaggctact cttacaaaat
12180tgtcagaact cgacatagtt ctccacttcc aacacctggc cgcagtggag
gcggactctt 12240gtcgcttcct cagctcacga cttgtgatgc tgaaaaatct
tgccgttggc aatgtgagct 12300tgcagtacaa caccacgttg aaccgcgttg
agctcatcct ccccacacca ggtacgaggc 12360ccaaattgac cgatttcaga
caatggctca tcagtgtgca cgcttccatt ttttcctctg 12420tagcctcatc
agttactttg ttcatagtgc tttggcttcg aattccagcc gtacgctatg
12480tttttggttt ccattggccc atggcaacac gtcattcgag ctgaccatta
attacactat 12540atgcatgccc tgtcttacca gccaagcggc tcaacaaagg
ctcgaacccg gtcgtaacat 12600gtggtgcaaa ataggacaca ccacgtgcga
ggagcgtgac catgatgagt tgtcaatgtc 12660catcccgtcc gggtacgaca
acctcaaact tgaaggttat tacgcttggc tggctttttt 12720gtccttttcc
tacgcggccc aattccatcc ggagttgttt ggaataggga atgtgtcgcg
12780cgtctttgtg gataaacgac accagttcat ttgtgccgag catgacggag
ataattcaac 12840cgtatctacc ggacacaaca tctccgcatc atatgcggca
tattatcacc accaaataga 12900cgggggcaat tggttccatt tggaatggct
gcggccgctc ttttcctctt ggctagtgct 12960caacatatca tggtttctga
ggcgttcgcc tgcaagccct gtttctcgac gcatctatca 13020gatattaaga
ccaatacgac cgcggctgcc ggtttcatgg tccttcaaga catcagttgc
13080ctccgacctc acagggtctc agcatcgcaa gagaacattc ccttcggaaa
gtcgtcacaa 13140tgtcgtgaag ccgtcggtac tccccagtac attacgatga
ctgctaatgt gaccgacgaa 13200tcatatttgt acaacgcgga cttgctaatg
ctttccgcgt gccttttcca cgcctcagaa 13260atgagcgaga aaggcttcaa
agttatcttt ggaaacgtct ccggcgttgt ttcagcttgt 13320gtcaatttca
cagattatgt ggcccatgta acccaacata cccaacagca tcatctggta
13380attgatcaca ttcggttact gcatttcctg acaccatctg caatgaggtg
ggctacaacc 13440attgcttgtt tgttcgccat tctcttagcg atatgagatg
ttctcacaaa ttggggcgtt 13500tcttgactcc gcactcttgc ttctggtggc
tttttttgct gtgtaccggc ttgtcctggt 13560cctttgccga tggcaacggc
aacagctcga cacgccaata catatataac ttgacgatat 13620gcgagctgaa
tgggaccgtc tggttgtcca gtcattttga ttgggcagtc gagacctttg
13680tgctttaccc ggtggccact catatcctct cactgggttt tctcacaaca
agccattttt 13740ttgatgcgct cggtctcggc gctgtgtcca ctacgggatt
tcttggcggg cggtatgtac 13800ttagcagcgt gtacggcgcc tgcgccttcg
cagcgcttgt atgttttgtc atccgtgctg 13860ctaaaaattg catggcttgc
cgttatgccc gcacccggtt caccaacttc atcgtggacg 13920accgggggaa
gatccatcga tggaagtccc caatagtggt agagaaatta ggcaaagctg
13980acatcggcgg cgaccttgtc accatcaaac atgttgtcct cgaaggagtc
aaagctcaac 14040ctttgacgag gacatcggcg gagcaatggg aagcctagat
gatttttgca atgatcctac 14100cgccgcacag aagcttgtgc tggcatttag
tatcacatac acacctataa tgatatacgc 14160cctcaaggtg tcacgcggcc
ggctcctagg actgttacac atcctgatat ttctgaactg 14220ttctttcacg
ttcggataca tgacatacgt gcactttcaa tccactaacc gtgtcgcgct
14280tactatgggg gcggtcgttg cccttttgtg gggcatttac agctttatag
aatcatggaa 14340gtttgtcact tccagatgca ggttgtgttg cctaggccgg
cgatacattc tggcccctgc 14400ccaccacgta gaaagtgctg caggcctcca
ttcaatccca gcgtctggta accgagcata 14460cgctgtgaga aagcccggac
taacatcagt gaacggcact ctagtaccag gacttcggag 14520cctcgtgttg
ggcggcaaac gagctgttaa acgaggagtg gttaacctcg tcaagtatgg
14580ccggtaaaaa ccagagccag aagaaaaaga aaaacacagc tcctatgggg
agtggccagc 14640cagtcaatca actgtgccaa ttgctgggca caatgataaa
gtcccagcgc cagcggccta 14700ggggaggaca ggccaaaatg aaaaagcctg
agaagccaca tttcccccta gctgctgaag 14760atgacatccg gcaccatttc
acccagaccg agcgttccct ttgcttgcaa tcgatccaga 14820cggccttcaa
tcaaggcgca ggaactgcgt cgctttcatc cagcgggaag gtcagttttc
14880aggttgagtt catgctgccg gtcgctcata cggtgcgcct gattcgcgta
acttccacat 14940ccgccagtca gggtgcaagt taatttgata gttaggtgaa
tggccgcgat tggcgtgtgg 15000cctctgagtc acctattcaa ttagggcgat
cacatggggg ttagacttaa ttggcgagaa 15060ccatgtgacc gaaattaaaa
aaaaaaaaaa aaaaaaaaaa aaaa 151043415101DNAPorcine reproductive and
respiratory syndrome virus 34atgatgtgta gggtattccc cctacataca
cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg
cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg
cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc
acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc
240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc
tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca
ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc
ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc
gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg
caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc
540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag
cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt
gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc
atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc
tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt
ggtcttcacg gactcctccc ccatgatgtg gacgccggaa tccgatgatt
840cagccgacct agaggcgcta ccgcctgagc tagaacgtca ggtcgaaatc
ctcattcgga 900gttttcctgc ccatcaccct gtcagcctgg ccgactggga
gctcgctgag tcccctgaga 960acggtttttc cttcagcacg taccattctg
gtggttatct tgtccaaaac cccgacgtgt 1020ttgacagcaa gtgctggctc
tcctgtttct tggatcagcc gatcgaagtg cgcctctatg 1080aggattatct
ggctaacgct ttcggttacc aaaccaagtg gggcgtgtct ggtaagtacc
1140tccagcgcag gcttcaagtc aacggtattc gtgctgtaat cgatcctgat
ggccccattc 1200acgttgaagc gctgtcttgc ccccaatctt ggatcaggca
cctgactctg gacgatgacg 1260tcaccccagg attcgttcgc ctaacatccc
tccgcattgt gccgaacaca gagcccacta 1320ctctccgaat ctttcggttt
ggagcgcata agtggtatgg cgctgccggc aaacgggctc 1380gtgccaagcg
tgccgctaaa agtgagaagg gtccggctcc cactcccaag gttgccccgc
1440cagcccccac ctgcggaatt gttacctact ctccaccaac agacgggtct
tgcggttggc 1500acgtccttgc cgccataatg aaccgaatga tgaacggtga
cttcatgtcc cctctggccc 1560agtacaacag accagaggat gattgggctt
ctgattacga tcttgcccag gcgattcaat 1620gtctgcggtt gcctgctacc
atagttcgga atcgtgcctg tcccaacgcc aagtacctca 1680taaaactcaa
cggagtccac tgggaggtag aggtgaggtc agggatggcc cctcgctccc
1740ttccccgcga gtgcgtagtc ggcgtttgtt ccgaaggctg tgccgcatcg
ccttacccag 1800aaaacgggct acctaaacga gcgtttgagg ccttggcgtc
tgcttacaga ctaccttccg 1860attgtgtcag ttctggtatt gctgactttc
ttgctaaccc ccctcaggaa ttctggactc 1920ttgacaaaat gttgacctcc
ccgtcaccgg agcggtctgg tttttccagt ctgtacaaat 1980tgctattaga
ggttgtcccg cagaaatgcg gagccacgga aggggctttc acctatgctg
2040ttgagagaat gctgaaggat tgcccgagct ccgaacaggc catggccctt
ctggcaaaaa 2100ttaaagttcc atcctcaaag gccccgtctg tgtccctgga
cgagtgtttc cctacggatg 2160tttcagccga tttcgaacca gcatctcagg
agaggtccca aaattccagc gctgctgttg 2220tcctgcattc accgaatgca
aaagagttcg aggaagcagc tccaggggaa gttcaggagg 2280gtggccacaa
ggccgtccac tctgcactcc ctgccggggg tcctaacaat aagcaggcac
2340agctggttgc cggtgagcaa ctgaagctcg gcggttgtgg ttcggtagtt
gggaatgcac 2400atgaaggtgt tctggtccca cctggtccaa ttaatttgac
aagcggggat ttaccctcct 2460caggctccat gaaagaagat atgctcaata
gccgggagga cgaaccactg gatttgtccc 2520aaccagcaac agctgtcaca
acgactctta tgggagagct aacacccgac tacctaggtt 2580ctgatactgg
tgccctcccc gtcaccgtcc gaaaatttgt cccgacgggg cctatactcc
2640gtcatgttga gcactgcagc acggggtcgg gcgatagcag ttcgcctttg
gatctgtctg 2700gtgcgcaaac cccggaccag cctttaaatc tgtccctggc
ggcttggcca gtgaggacca 2760ccgcgtctga tcctggctgg gtccacggta
gacgcgagcc tgtctttgta aagcctcgag 2820atgttttctc tgatggcgat
tcagcccttc agttcgggga gctttctgaa tccagctctg 2880tcatcgagtt
tgatcgggca aaagatgctc aggtggctga cgcccctgtc ggtctgacga
2940cttcggacga ggccctctcc gcagtcgatc ctttcgagtt ttccgaactc
aagcgcccac 3000gtttctccgc acaagcctta attgaccgag gcggcccact
tgccgatgtc catgcaaaaa 3060taaagaaccg ggtatatgaa cagtgcctcc
aagcttgtga gcccggtagt cgcgcaaccc 3120cagctaccag ggagtggctc
gacaaaatgt gggagagagt ggacatgaaa acctggcgct 3180gcacctcaca
gttccaagct ggtcacattc ttgcgtccct caaattcctc cctgacatga
3240ttcaagacac accgcctcct gttcccagga agaaccgagc cagtgataaa
gctggcctga 3300aacaactagt ggcacagtgg gataggagat tgagttcaac
ccccccccca aaaccggttg 3360ggccggtact tgaccgggtc gatcctccgc
ctacgggtac ccggcaagaa gacgtcaccc 3420cctccgatgg gccaccccat
gcgccggatg gtcgagtgag tacgggtggg agttggaaag 3480gccttatgct
ttccggcacc cgtctcacgg ggtccatcag tcatcgcctc atgacatggg
3540tttttgaagt tgtctcccat ctcccagctt ttatgctcac acttttctcg
ccgcggggct 3600ctatggctcc aggcgattgg gtttttgcag gtgttgtttt
acttgctctc ctgctctgtc 3660gttcttaccc aatattcggg tgccttccct
tattgggtgt cttttctggt actgtgcggc 3720gtgttcgtct gggtgttttt
ggctcttgga tggcttttgc tgtattttta ttctcgactc 3780catccaaccc
agtcggttct tcttgtaacc acgattcgcc ggagtgtcat gctgagcttc
3840tggctcttga gcagcgccaa ctttgggaac ctgtgcgcgg ccttgtggtc
ggcccatcgg 3900gcctcttatg tgtcattctt ggcaagttac tcggtgggtc
acgttatctc tggcatgttc 3960tcctacgtct atgcctgctt gcagatttgg
ccctttctct tgtttatgtg gtgtcccagg 4020ggcgttgtca caagtgttgg
ggaaagtgta taaggacagc tcctgctgag gtggctttta 4080atgtatttcc
tttctcgcgc gccacccgta gttctcttgt atccttatgt gatcgattcc
4140aaacgccaaa aggagttgat cccgtgcact tggcgacagg ttggcgcggg
tgctggcgtg 4200gtgagagtcc tatccatcaa ccacaccaaa agcccatagc
ttacgccaat ttggatgaaa 4260agaagatatc tgcccaaacg gttgttgctg
tcccatacga tcccaatcag gctatcaaat 4320gtctgaaagt tctgcaggcg
ggaggggcta tcgtggatca gcctacgcct gaggtcgttc 4380gtgtgtccga
gatccccttc tcagccccat ttttcccaaa agttccagtc aacccagatt
4440gtagggtcgt ggtggattcg gacacttttg tggctgcggt tcgttgtggt
tattcgacat 4500cacaactggt cctgggccag ggcaactttg ccaagttaaa
tcaaaccccc cccaggaact 4560ctatctccac caaagcgact ggtggggcct
cttatacttt tgctgtggct caagtgtctg 4620tgtggaccct tgttcatttc
gtcctcggtc tttggctcac gtcacctcaa gtgtgtggtc 4680gaggaaccgc
tgacccatgg tgttcaagtc cattttcata tcctacctat ggccccgggg
4740ttgtgtgctc ctctcgactt tgtgtgtctg ctgacggggt cacccttcca
ttgttctcag 4800ccgtggcaca actctctggt agggaggtgg ggatttttat
tttagtgctc gtctccttta 4860ttgccttggc ccaccgcatg gctcttaagg
cagacatgtt agtagtcttt ttggctcttt 4920gtgcttatgc ctggcccatg
agctcctggt tgatctgctt ctttcctata ctcttgaggt 4980gggttaccct
tcaccctctc actatgcttt gggtgcattc attcttgatg ttttgtctcc
5040cagcagccgg cgtcctctca ctggggataa ctggcctcct ctgggcaatc
ggccgcttta 5100ctcaggttgc cggaatcatt acaccttatg atatccacca
gtacacctct gggccacgtg 5160gtgcagccgc tgtggccaca gccccagaag
gcacttacat ggccgccgtc cggagagctg 5220ctttaaccgg gcggacttta
atcttcaccc cgtccgcagt tggatccctt ctcgaaggtg 5280ctttcaggac
tcataaaccc tgccttaaca ccgtgaatgt cgtaggctcc tcccttggtt
5340ccggaggggt tttcaccatt gacggaaaaa aaattgtcgt cactgctgcc
catgtgctga 5400acggcgacac agctagagtc accggtgatt cctacaaccg
catgcacact ttcaagacta 5460atggtgacta tgcctggtcc catgctgata
actggcaggg cgctgcccct gtggtcaagg 5520ttgcgaaagg gtatcgcggt
cgtgcctact ggcaaacatc aactggtgtc gagcctggtg 5580ttattgggaa
tgggttcgcc ttctgtttca ccaactgcgg cgattcgggg tcacccgtta
5640tctcagaatc tggtgatctt atcggaatcc acaccggttc aaacaaactt
ggttctggtc 5700ttgtgacaac ccctgaaggg gagacctgta ccatcagaga
aaccaagctt tctgaccttt 5760ccagacattt cgcaggccca agcgttcctc
ttggggacat caaattgagt ccggccatca 5820tccctgatgt gacatccatt
ccgagtgact tggcatcgct cctagcttcc gtccctgtaa 5880tggaaggcgg
cctctcgacc gttcaacttt tgtgtgtctt tttcctcctc tggcgcatga
5940tgggccatgc ctggacgccc attgttgccg tgggcttctt tttgctgaat
gaaattcttc 6000cagcagtttt ggttcgagcc gtgttttctt ttgcactctt
tgtgcttgca tgggccaccc 6060cctggtctgc acaagtgttg atgattagac
ttctcacggc atctctcaac cgcaacaaac 6120tttctcttgc gttctacgca
ctcggaggtg ttgttggttt
ggctgctgaa atcgggactt 6180ttgctggtaa attgtctgaa ttgtctcaag
ctctttcgac atactgtttc ttacctaggg 6240tccttgctat gaccagctgt
gttcccatca tcatcattgg tggactccat gccctcggtg 6300tgattctgtg
gttattcaaa taccggtgcc tccacaacat gctggttggt gatggaagct
6360tttcaagcgc tttcttccta cggtattttg cagagggcaa tctcaggaga
ggtgtttcac 6420agtcctgtgg catgagtaac gagtccctga cggctgcttt
ggcttgcaag ttgtcacagg 6480ctgaccttga ttttttgtcc agcttaacga
acttcaagtg ctttgtatct gcttcaaaca 6540tgaaaaatgc tgccggccag
tacattgaag cagcttatgc cagggccctg cgtcaagagt 6600tggcctcttt
agtccagatt gacaaaatga aaggagtttt gtccaagcta gaggcctttg
6660ctgaaacggc cactccgtcc ctcgacgtag gtgacgtgat tgttctactt
ggacaacatc 6720ctcacggatc cgttctcgat attaatgtgg ggactgaaag
gaaaactgta tccgtgcaag 6780agacccggag cctaggcggc tccaagttca
gtgtttgtac tgttgtgtca aacacacccg 6840tggacgcctt agccggtatt
ccactccaga caccaacccc ccttttcgag aatggcccgc 6900gtcatcgcag
cgaggaggac gatcttaaag tcgagaggat gaagaaacac tgcgtgtccc
6960tcggcttcca caacattaac ggtaaagttt actgcaagat ttgggacaag
tctaccggtg 7020acgcctttta cactgatgat tcccggtaca cccaagacta
tgcttttcag gacaggtcag 7080ctgactatag agacagggac tacgagggtg
tgcaaaccgc cccccaacag ggatttgatc 7140caaagtctga aacccctgtt
ggtaccgttg tgatcggcgg tattacgtac aacaggtatt 7200tggtcaaagg
taaggaggtt ctggttccca agcctgacaa ctgccttgaa gctgccaagc
7260tgtcccttga gcaagctctc gctgggatgg gccaaacttg tgaccttaca
gctgccgagg 7320tggaaaagct aaagcgcatc attggtcaac ttcaaggatt
gaccactgag caggctttaa 7380actgttagcc gccagcggct tgacccgctg
tggccgcggc ggcctagttg taactgaaac 7440ggcggtaaaa attgtcaaat
accacagcag aacttttacc ttaggctctt tagacctaaa 7500agtcacttcc
gaggtggagg tgaagaagtc aaccgagcag ggccacgctg ttgtggcaaa
7560cttgtgttct ggtgtcgtct tgatgagacc tcacccaccg tcccttgtcg
acgttcttct 7620gaaacccgga cttgacataa cacccggcat tcaaccaggg
catggggccg ggaatatggg 7680cgtggacggt tccatttggg attttgaaac
cgcacccaca aaggctgaac tcgagttatc 7740caagcaaata attcaagcat
gtgaagtcag gcgcggggat gccccgaacc tccagctccc 7800ttacaagctc
tatcctgtta gaggggatcc tgagcggcat aaaggccacc tcatcaatac
7860caggtttgga gacttacctt acaaaactcc tcaagacacc aagtccgcaa
tccacgcggc 7920ttgttgcctg caccccaacg gggcccccgt gtctgatggt
aaatccacac taggtaccac 7980tcttcaacat ggcttcgagc tttatgtccc
tactgtgccc tatagtgtca tggagtacct 8040tgattcacgc ccagacaccc
cttttatgtg cactaaacac ggcacttcca aggctgctgc 8100agaggacctc
caaaaatacg acctatccac ccaaggattt gtcctgcctg gggtcctacg
8160cctagtgcgc aggttcatct ttggccacat tggcaaggca ccgccattgt
tcctcccatc 8220aacttatccc gccaagaact ccatggcagg tattaatggt
cagaggttcc caacaaagga 8280tgttcaaagt atacctgaaa ttgatgaaat
gtgtgcccgc gccgtcaagg agaattggca 8340aactgtgaca ccttgcaccc
tcaagaaaca gtattgttct aggcccaaaa ccaggaccat 8400cctgggcact
aacaacttca tagccttggc tcatagatcg gcgctcagtg gtgttaccca
8460ggcattcatg aagaaggctt ggaagtcccc aatagcctta gggaaaaaca
aattcaagga 8520gctgcattgc actgtcgctg gcaggtgcct cgaggccgac
ttggcttcct gtgaccgcag 8580cacccctgcc attgtgaggt ggttcactac
ccacctccta tatgaacttg caggatgtga 8640agaatatcta cctagctatg
tgcttaactg ttgccatgac cttgtggcga cgcaggatgg 8700tgctttcaca
aaacgcggtg gcctgtcgtc tggagaccca gtcaccagtg tgtccaacac
8760tgtgtactca ctggtgattt atgcccagca catggtacta tctgccctga
aaatgggtca 8820tgaaattggc ctcaagttcc tcgaagaaca actcaaattt
gaggaccttc ttgaaatcca 8880gcctatgtta gtatactctg atgatcttgt
cttgtacgca gaaaagccca ccttccccaa 8940ctatcattgg tgggtcgagc
atcttgacct gatgttgggc tttaaaacgg acccaaagaa 9000aaccgtcata
actgataaac ccagtttcct cgggtgcaga atcgaagcag ggcgacagct
9060agtccccaat cgcgaccgca tcctggctgc tcttgcatat cacatgaagg
cgcagaacgc 9120ctcagagtat tatgcgtctg ctgccgcaat cctgatggat
tcatgtgctt gcattgacca 9180cgatcctgaa tggtatgagg acctcatctg
tggcattgcc cgatgcgctc gcctggacgg 9240ttatagcttt ccaggtccgg
catttttcat gtccatgtgg gagaagctga ggagtcataa 9300tgaagggaag
aaattccgcc actgcggcat ctgcgacgcc aaagccgact acgcggctgc
9360ttgtgggctt gatttgtgtt tgtttcattc gcactttcac caacactgcc
ctgtcactct 9420gagctgcggt caccatgccg gttcgaagga atgttcgcag
tgtcagtcac ctgttggggc 9480cggcagatcc cctcttgatg ctgtgctgga
acaaattcca tacaaacctc ctcgcactgt 9540catcatgaag gtgggtaata
aaacaacggc ccttgatccg gggaggtacc agtcccgtcg 9600aggtctcgtt
gcagtcaaga ggggtattgc gggtaatgaa gttgatcttg ctgatggaga
9660ttaccaagtg gtacctcttc tgccgacttg caaagatata aacatggtga
aggtggcttg 9720caacgtacta ctcagcaagt tcatagtagg gccaccaggt
tccgggaaga ccacctggct 9780actaagtcaa gttcaggacg atgatgtcat
ttacacaccc actcatcaga ccatgtttga 9840catagttagt gctctcaaag
tttgcaggta ttccattcca ggagcctcgg gactcccttt 9900cccaccgcct
gccaggtccg ggccgtgggt taggctcatt gccagcgggc acgtccctgg
9960ccgagtatca tacctcgatg aggccggata ttgcaatcat ctggacattc
ttagactgct 10020ttccaaaaca ccccttgtgt gtttgggtga ccttcagcaa
cttcacccag tcggctttga 10080ttcctattgt tatgtgttcg atcagatgcc
tcagaagcag ttgaccacca tttacagatt 10140tggccccaac atctgcgcag
ccatccagcc ttgttacagg gagaaacttg aatctaaggc 10200taggaacacc
agggtggttt ttaccacccg gcctgtggcc tttggccagg tgctgacacc
10260atatcacaaa gatcgcgtcg gctccgcgat taccatagac tcatcccagg
gggccacctt 10320tgacattgta acattgcatc taccatcgcc aaagtcccta
aataagtccc gggcacttgt 10380ggccatcaca cgggcaagac acgggttgtt
catttatgac cctcacaacc agctccggga 10440gtttttcaac ctaacccctg
agcgcactga ttgtaacctt gtgttcagcc gtggagatga 10500gctggtggtc
ctgaatgcag ataatgcagt cacaaccgtg gcgaaggcct tagagacagg
10560tccaactcaa tttcgagtgt cagacccgag gtgcaagtct ctcttagccg
cttgctcggc 10620cagtctggaa gggagctgca tgccgctacc gcaagtggcg
cataacctgg ggttttactt 10680ctccccagac agtccagtat ttgcacctct
gccaaaagag ttggcgccac attggccagt 10740ggttacccat cagaataatc
gggcgtggcc tgatcgactt gtcgctagta tgcgtccaat 10800tgacgcccgc
tacagcaagc cgatggtcgg tgcagggtat gtggtcggac cgtccacctt
10860ccttggtact cctggagtgg tgtcatacta tctcacacta tacatcaggg
gtgagcccca 10920ggccttgcca gaaacacttg tttcaacagg acgtatagcc
acagattgtc gggagtatct 10980cgacgcggct gaggaagagg cagcaaaaga
acttccccac gcgttcattg gcgatgtcaa 11040aggcaccaca gttggggggt
gtcatcacat tacatcaaaa tacctaccta ggtccctgcc 11100taaagactct
gttgccgtag ttggagtaag ttcgcctggc agggctgcta aagccgtatg
11160caccctcacc gatgtgtacc tccctgaact ccggccatat ctgcaacctg
agacggcatc 11220aaaatgctgg aaactcaaat tagacttcag ggacgtccga
ctaatggtct ggaaaggagc 11280caccgcctac tttcaattgg aagggctcac
atggtcggcg ctgcctgact atgccaggtt 11340tattcagctg cccaaaaacg
ctgttgtata catcgatccg tgcataggac cggcaacagc 11400caatcgtaaa
gtcgtacgaa ccacagattg gcgggccgac ctggcagtga cgccgtatga
11460ttacggtgcc cggaacattt tgacaacagc ctggttcgag gacctcgggc
cgcagtggaa 11520gattctgggg ttgcagccct ttaggcgggc gtttggcttt
gaaaacactg aggattgggc 11580aatccttgca tgctgcatga gtgacggcaa
ggactacact gactataact ggaattgcgt 11640tcgacaacgc ccacacgcta
tccatggacg cgctcgtgac catacgtacc actttgcccc 11700tggcactgaa
ttgcaagtgg agctcggtaa accccggctg ccacctgagc aagtaccgtg
11760aattcggagt gatgcaatgg ggtcactgtg gagtaaaatc agccagctgt
tcgtggacgc 11820cttcactgaa ttccttgtta gtgtggttga tattgtcatc
ttccttgcca tattgtttgg 11880gttcaccgtc gcaggatggt tactggtctt
tcttctcaga gtggtttgct ccgcgcttct 11940ccgttcgcgc tctgccattc
actcttccga actatcgaag gtcctatgag ggcttactac 12000ctaattgcag
accggatgtt ccacaatttg catttaagca ccctttgggt atgttttggc
12060acatgcgggt ttcccaccta attgatcaga tggtctctcg ccgcatctac
cagaccatgg 12120aacattcagg tcaagcggcc tggaagcacg tggtcagtga
ggctactctt acaaaattgt 12180cagaactcga catagttctc cacttccaac
acctggccgc agtggaggcg gactcttgtc 12240gcttcctcag ctcacgactt
gtgatgctga aaaatcttgc cgttggcaat gtgagcttgc 12300agtacaacac
cacgttgaac cgcgttgagc tcatcctccc cacaccaggt acgaggccca
12360aattgaccga tttcagacaa tggctcatca gtgtgcacgc ttccattttt
tcctctgtag 12420cctcatcagt tactttgttc atagtgcttt ggcttcgaat
tccagccgta cgctatgttt 12480ttggtttcca ttggcccatg gcaacacgtc
attcgagctg accattaatt acactatatg 12540catgccctgt cttaccagcc
aagcggctca acaaaggctc gaacccggtc gtaacatgtg 12600gtgcaaaata
ggacacacca cgtgcgagga gcgtgaccat gatgagttgt caatgtccat
12660cccgtccggg tacgacaacc tcaaacttga aggttattac gcttggctgg
cttttttgtc 12720cttttcctac gcggcccaat tccatccgga gttgtttgga
atagggaatg tgtcgcgcgt 12780ctttgtggat aaacgacacc agttcatttg
tgccgagcat gacggagata attcaaccgt 12840atctaccgga cacaacatct
ccgcatcata tgcggcatat tatcaccacc aaatagacgg 12900gggcaattgg
ttccatttgg aatggctgcg gccgctcttt tcctcttggc tagtgctcaa
12960catatcatgg tttctgaggc gttcgcctgc aagccctgtt tctcgacgca
tctatcagat 13020attaagacca atacgaccgc ggctgccggt ttcatggtcc
ttcaagacat cagttgcctc 13080cgacctcaca gggtctcagc atcgcaagag
aacattccct tcggaaagtc gtcacaatgt 13140cgtgaagccg tcggtactcc
ccagtacatt acgatgactg ctaatgtgac cgacgaatca 13200tatttgtaca
acgcggactt gctaatgctt tccgcgtgcc ttttccacgc ctcagaaatg
13260agcgagaaag gcttcaaagt tatctttgga aacgtctccg gcgttgtttc
agcttgtgtc 13320aatttcacag attatgtggc ccatgtaacc caacataccc
aacagcatca tctggtaatt 13380gatcacattc ggttactgca tttcctgaca
ccatctgcaa tgaggtgggc tacaaccatt 13440gcttgtttgt tcgccattct
cttagcgata tgagatgttc tcacaaattg gggcgtttct 13500tgactccgca
ctcttgcttc tggtggcttt ttttgctgtg taccggcttg tcctggtcct
13560ttgccgatgg caacggcaac agctcgacac gccaatacat atataacttg
acgatatgcg 13620agctgaatgg gaccgtctgg ttgtccagtc attttgattg
ggcagtcgag acctttgtgc 13680tttacccggt ggccactcat atcctctcac
tgggttttct cacaacaagc catttttttg 13740atgcgctcgg tctcggcgct
gtgtccacta cgggatttct tggcgggcgg tatgtactta 13800gcagcgtgta
cggcgcctgc gccttcgcag cgcttgtatg ttttgtcatc cgtgctgcta
13860aaaattgcat ggcttgccgt tatgcccgca cccggttcac caacttcatc
gtggacgacc 13920gggggaagat ccatcgatgg aagtccccaa tagtggtaga
gaaattaggc aaagctgaca 13980tcggcggcga ccttgtcacc atcaaacatg
ttgtcctcga aggagtcaaa gctcaacctt 14040tgacgaggac atcggcggag
caatgggaag cctagatgat ttttgcaatg atcctaccgc 14100cgcacagaag
cttgtgctgg catttagtat cacatacaca cctataatga tatacgccct
14160caaggtgtca cgcggccggc tcctaggact gttacacatc ctgatatttc
tgaactgttc 14220tttcacgttc ggatacatga catacgtgca ctttcaatcc
actaaccgtg tcgcgcttac 14280tatgggggcg gtcgttgccc ttttgtgggg
catttacagc tttatagaat catggaagtt 14340tgtcacttcc agatgcaggt
tgtgttgcct aggccggcga tacattctgg cccctgccca 14400ccacgtagaa
agtgctgcag gcctccattc aatcccagcg tctggtaacc gagcatacgc
14460tgtgagaaag cccggactaa catcagtgaa cggcactcta gtaccaggac
ttcggagcct 14520cgtgttgggc ggcaaacgag ctgttaaacg aggagtggtt
aacctcgtca agtatggccg 14580gtaaaaacca gagccagaag aaaaagaaaa
acacagctcc tatggggagt ggccagccag 14640tcaatcaact gtgccaattg
ctgggcacaa tgataaagtc ccagcgccag cggcctaggg 14700gaggacaggc
caaaatgaaa aagcctgaga agccacattt ccccctagct gctgaagatg
14760acatccggca ccatttcacc cagaccgagc gttccctttg cttgcaatcg
atccagacgg 14820ccttcaatca aggcgcagga actgcgtcgc tttcatccag
cgggaaggtc agttttcagg 14880ttgagttcat gctgccggtc gctcatacgg
tgcgcctgat tcgcgtaact tccacatccg 14940ccagtcaggg tgcaagttaa
tttgatagtt aggtgaatgg ccgcgattgg cgtgtggcct 15000ctgagtcacc
tattcaatta gggcgatcac atgggggtta gacttaattg gcgagaacca
15060tgtgaccgaa attaaaaaaa aaaaaaaaaa aaaaaaaaaa a
151013515098DNAPorcine reproductive and respiratory syndrome virus
35atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag
60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct
120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct
cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac
catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat
tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct
cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt
ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc
420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc
ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa
agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc
gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg
ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca
gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac
720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg
tacaggtgga 780aaaaatttgt ggtcttcacg gactcctcca tgatgtggac
gccggaatcc gatgattcag 840ccgacctaga ggcgctaccg cctgagctag
aacgtcaggt cgaaatcctc attcggagtt 900ttcctgccca tcaccctgtc
agcctggccg actgggagct cgctgagtcc cctgagaacg 960gtttttcctt
cagcacgtac cattctggtg gttatcttgt ccaaaacccc gacgtgtttg
1020acagcaagtg ctggctctcc tgtttcttgg atcagccgat cgaagtgcgc
ctctatgagg 1080attatctggc taacgctttc ggttaccaaa ccaagtgggg
cgtgtctggt aagtacctcc 1140agcgcaggct tcaagtcaac ggtattcgtg
ctgtaatcga tcctgatggc cccattcacg 1200ttgaagcgct gtcttgcccc
caatcttgga tcaggcacct gactctggac gatgacgtca 1260ccccaggatt
cgttcgccta acatccctcc gcattgtgcc gaacacagag cccactactc
1320tccgaatctt tcggtttgga gcgcataagt ggtatggcgc tgccggcaaa
cgggctcgtg 1380ccaagcgtgc cgctaaaagt gagaagggtc cggctcccac
tcccaaggtt gccccgccag 1440cccccacctg cggaattgtt acctactctc
caccaacaga cgggtcttgc ggttggcacg 1500tccttgccgc cataatgaac
cgaatgatga acggtgactt catgtcccct ctggcccagt 1560acaacagacc
agaggatgat tgggcttctg attacgatct tgcccaggcg attcaatgtc
1620tgcggttgcc tgctaccata gttcggaatc gtgcctgtcc caacgccaag
tacctcataa 1680aactcaacgg agtccactgg gaggtagagg tgaggtcagg
gatggcccct cgctcccttc 1740cccgcgagtg cgtagtcggc gtttgttccg
aaggctgtgc cgcatcgcct tacccagaaa 1800acgggctacc taaacgagcg
tttgaggcct tggcgtctgc ttacagacta ccttccgatt 1860gtgtcagttc
tggtattgct gactttcttg ctaacccccc tcaggaattc tggactcttg
1920acaaaatgtt gacctccccg tcaccggagc ggtctggttt ttccagtctg
tacaaattgc 1980tattagaggt tgtcccgcag aaatgcggag ccacggaagg
ggctttcacc tatgctgttg 2040agagaatgct gaaggattgc ccgagctccg
aacaggccat ggcccttctg gcaaaaatta 2100aagttccatc ctcaaaggcc
ccgtctgtgt ccctggacga gtgtttccct acggatgttt 2160cagccgattt
cgaaccagca tctcaggaga ggtcccaaaa ttccagcgct gctgttgtcc
2220tgcattcacc gaatgcaaaa gagttcgagg aagcagctcc aggggaagtt
caggagggtg 2280gccacaaggc cgtccactct gcactccctg ccgggggtcc
taacaataag caggcacagc 2340tggttgccgg tgagcaactg aagctcggcg
gttgtggttc ggtagttggg aatgcacatg 2400aaggtgttct ggtcccacct
ggtccaatta atttgacaag cggggattta ccctcctcag 2460gctccatgaa
agaagatatg ctcaatagcc gggaggacga accactggat ttgtcccaac
2520cagcaacagc tgtcacaacg actcttatgg gagagctaac acccgactac
ctaggttctg 2580atactggtgc cctccccgtc accgtccgaa aatttgtccc
gacggggcct atactccgtc 2640atgttgagca ctgcagcacg gggtcgggcg
atagcagttc gcctttggat ctgtctggtg 2700cgcaaacccc ggaccagcct
ttaaatctgt ccctggcggc ttggccagtg aggaccaccg 2760cgtctgatcc
tggctgggtc cacggtagac gcgagcctgt ctttgtaaag cctcgagatg
2820ttttctctga tggcgattca gcccttcagt tcggggagct ttctgaatcc
agctctgtca 2880tcgagtttga tcgggcaaaa gatgctcagg tggctgacgc
ccctgtcggt ctgacgactt 2940cggacgaggc cctctccgca gtcgatcctt
tcgagttttc cgaactcaag cgcccacgtt 3000tctccgcaca agccttaatt
gaccgaggcg gcccacttgc cgatgtccat gcaaaaataa 3060agaaccgggt
atatgaacag tgcctccaag cttgtgagcc cggtagtcgc gcaaccccag
3120ctaccaggga gtggctcgac aaaatgtggg agagagtgga catgaaaacc
tggcgctgca 3180cctcacagtt ccaagctggt cacattcttg cgtccctcaa
attcctccct gacatgattc 3240aagacacacc gcctcctgtt cccaggaaga
accgagccag tgataaagct ggcctgaaac 3300aactagtggc acagtgggat
aggagattga gttcaacccc ccccccaaaa ccggttgggc 3360cggtacttga
ccgggtcgat cctccgccta cgggtacccg gcaagaagac gtcaccccct
3420ccgatgggcc accccatgcg ccggatggtc gagtgagtac gggtgggagt
tggaaaggcc 3480ttatgctttc cggcacccgt ctcacggggt ccatcagtca
tcgcctcatg acatgggttt 3540ttgaagttgt ctcccatctc ccagctttta
tgctcacact tttctcgccg cggggctcta 3600tggctccagg cgattgggtt
tttgcaggtg ttgttttact tgctctcctg ctctgtcgtt 3660cttacccaat
attcgggtgc cttcccttat tgggtgtctt ttctggtact gtgcggcgtg
3720ttcgtctggg tgtttttggc tcttggatgg cttttgctgt atttttattc
tcgactccat 3780ccaacccagt cggttcttct tgtaaccacg attcgccgga
gtgtcatgct gagcttctgg 3840ctcttgagca gcgccaactt tgggaacctg
tgcgcggcct tgtggtcggc ccatcgggcc 3900tcttatgtgt cattcttggc
aagttactcg gtgggtcacg ttatctctgg catgttctcc 3960tacgtctatg
cctgcttgca gatttggccc tttctcttgt ttatgtggtg tcccaggggc
4020gttgtcacaa gtgttgggga aagtgtataa ggacagctcc tgctgaggtg
gcttttaatg 4080tatttccttt ctcgcgcgcc acccgtagtt ctcttgtatc
cttatgtgat cgattccaaa 4140cgccaaaagg agttgatccc gtgcacttgg
cgacaggttg gcgcgggtgc tggcgtggtg 4200agagtcctat ccatcaacca
caccaaaagc ccatagctta cgccaatttg gatgaaaaga 4260agatatctgc
ccaaacggtt gttgctgtcc catacgatcc caatcaggct atcaaatgtc
4320tgaaagttct gcaggcggga ggggctatcg tggatcagcc tacgcctgag
gtcgttcgtg 4380tgtccgagat ccccttctca gccccatttt tcccaaaagt
tccagtcaac ccagattgta 4440gggtcgtggt ggattcggac acttttgtgg
ctgcggttcg ttgtggttat tcgacatcac 4500aactggtcct gggccagggc
aactttgcca agttaaatca aacccccccc aggaactcta 4560tctccaccaa
agcgactggt ggggcctctt atacttttgc tgtggctcaa gtgtctgtgt
4620ggacccttgt tcatttcgtc ctcggtcttt ggctcacgtc acctcaagtg
tgtggtcgag 4680gaaccgctga cccatggtgt tcaagtccat tttcatatcc
tacctatggc cccggggttg 4740tgtgctcctc tcgactttgt gtgtctgctg
acggggtcac ccttccattg ttctcagccg 4800tggcacaact ctctggtagg
gaggtgggga tttttatttt agtgctcgtc tcctttattg 4860ccttggccca
ccgcatggct cttaaggcag acatgttagt agtctttttg gctctttgtg
4920cttatgcctg gcccatgagc tcctggttga tctgcttctt tcctatactc
ttgaggtggg 4980ttacccttca ccctctcact atgctttggg tgcattcatt
cttgatgttt tgtctcccag 5040cagccggcgt cctctcactg gggataactg
gcctcctctg ggcaatcggc cgctttactc 5100aggttgccgg aatcattaca
ccttatgata tccaccagta cacctctggg ccacgtggtg 5160cagccgctgt
ggccacagcc ccagaaggca cttacatggc cgccgtccgg agagctgctt
5220taaccgggcg gactttaatc ttcaccccgt ccgcagttgg atcccttctc
gaaggtgctt 5280tcaggactca taaaccctgc cttaacaccg tgaatgtcgt
aggctcctcc cttggttccg 5340gaggggtttt caccattgac ggaaaaaaaa
ttgtcgtcac tgctgcccat gtgctgaacg 5400gcgacacagc tagagtcacc
ggtgattcct acaaccgcat gcacactttc aagactaatg 5460gtgactatgc
ctggtcccat gctgataact ggcagggcgc tgcccctgtg gtcaaggttg
5520cgaaagggta tcgcggtcgt gcctactggc aaacatcaac tggtgtcgag
cctggtgtta 5580ttgggaatgg gttcgccttc tgtttcacca actgcggcga
ttcggggtca cccgttatct 5640cagaatctgg tgatcttatc ggaatccaca
ccggttcaaa caaacttggt tctggtcttg 5700tgacaacccc tgaaggggag
acctgtacca tcagagaaac caagctttct gacctttcca 5760gacatttcgc
aggcccaagc gttcctcttg gggacatcaa attgagtccg gccatcatcc
5820ctgatgtgac atccattccg agtgacttgg catcgctcct agcttccgtc
cctgtaatgg 5880aaggcggcct ctcgaccgtt caacttttgt gtgtcttttt
cctcctctgg cgcatgatgg 5940gccatgcctg gacgcccatt gttgccgtgg
gcttcttttt gctgaatgaa attcttccag 6000cagttttggt tcgagccgtg
ttttcttttg cactctttgt gcttgcatgg
gccaccccct 6060ggtctgcaca agtgttgatg attagacttc tcacggcatc
tctcaaccgc aacaaacttt 6120ctcttgcgtt ctacgcactc ggaggtgttg
ttggtttggc tgctgaaatc gggacttttg 6180ctggtaaatt gtctgaattg
tctcaagctc tttcgacata ctgtttctta cctagggtcc 6240ttgctatgac
cagctgtgtt cccatcatca tcattggtgg actccatgcc ctcggtgtga
6300ttctgtggtt attcaaatac cggtgcctcc acaacatgct ggttggtgat
ggaagctttt 6360caagcgcttt cttcctacgg tattttgcag agggcaatct
caggagaggt gtttcacagt 6420cctgtggcat gagtaacgag tccctgacgg
ctgctttggc ttgcaagttg tcacaggctg 6480accttgattt tttgtccagc
ttaacgaact tcaagtgctt tgtatctgct tcaaacatga 6540aaaatgctgc
cggccagtac attgaagcag cttatgccag ggccctgcgt caagagttgg
6600cctctttagt ccagattgac aaaatgaaag gagttttgtc caagctagag
gcctttgctg 6660aaacggccac tccgtccctc gacgtaggtg acgtgattgt
tctacttgga caacatcctc 6720acggatccgt tctcgatatt aatgtgggga
ctgaaaggaa aactgtatcc gtgcaagaga 6780cccggagcct aggcggctcc
aagttcagtg tttgtactgt tgtgtcaaac acacccgtgg 6840acgccttagc
cggtattcca ctccagacac caacccccct tttcgagaat ggcccgcgtc
6900atcgcagcga ggaggacgat cttaaagtcg agaggatgaa gaaacactgc
gtgtccctcg 6960gcttccacaa cattaacggt aaagtttact gcaagatttg
ggacaagtct accggtgacg 7020ccttttacac tgatgattcc cggtacaccc
aagactatgc ttttcaggac aggtcagctg 7080actatagaga cagggactac
gagggtgtgc aaaccgcccc ccaacaggga tttgatccaa 7140agtctgaaac
ccctgttggt accgttgtga tcggcggtat tacgtacaac aggtatttgg
7200tcaaaggtaa ggaggttctg gttcccaagc ctgacaactg ccttgaagct
gccaagctgt 7260cccttgagca agctctcgct gggatgggcc aaacttgtga
ccttacagct gccgaggtgg 7320aaaagctaaa gcgcatcatt ggtcaacttc
aaggattgac cactgagcag gctttaaact 7380gttagccgcc agcggcttga
cccgctgtgg ccgcggcggc ctagttgtaa ctgaaacggc 7440ggtaaaaatt
gtcaaatacc acagcagaac ttttacctta ggctctttag acctaaaagt
7500cacttccgag gtggaggtga agaagtcaac cgagcagggc cacgctgttg
tggcaaactt 7560gtgttctggt gtcgtcttga tgagacctca cccaccgtcc
cttgtcgacg ttcttctgaa 7620acccggactt gacataacac ccggcattca
accagggcat ggggccggga atatgggcgt 7680ggacggttcc atttgggatt
ttgaaaccgc acccacaaag gctgaactcg agttatccaa 7740gcaaataatt
caagcatgtg aagtcaggcg cggggatgcc ccgaacctcc agctccctta
7800caagctctat cctgttagag gggatcctga gcggcataaa ggccacctca
tcaataccag 7860gtttggagac ttaccttaca aaactcctca agacaccaag
tccgcaatcc acgcggcttg 7920ttgcctgcac cccaacgggg cccccgtgtc
tgatggtaaa tccacactag gtaccactct 7980tcaacatggc ttcgagcttt
atgtccctac tgtgccctat agtgtcatgg agtaccttga 8040ttcacgccca
gacacccctt ttatgtgcac taaacacggc acttccaagg ctgctgcaga
8100ggacctccaa aaatacgacc tatccaccca aggatttgtc ctgcctgggg
tcctacgcct 8160agtgcgcagg ttcatctttg gccacattgg caaggcaccg
ccattgttcc tcccatcaac 8220ttatcccgcc aagaactcca tggcaggtat
taatggtcag aggttcccaa caaaggatgt 8280tcaaagtata cctgaaattg
atgaaatgtg tgcccgcgcc gtcaaggaga attggcaaac 8340tgtgacacct
tgcaccctca agaaacagta ttgttctagg cccaaaacca ggaccatcct
8400gggcactaac aacttcatag ccttggctca tagatcggcg ctcagtggtg
ttacccaggc 8460attcatgaag aaggcttgga agtccccaat agccttaggg
aaaaacaaat tcaaggagct 8520gcattgcact gtcgctggca ggtgcctcga
ggccgacttg gcttcctgtg accgcagcac 8580ccctgccatt gtgaggtggt
tcactaccca cctcctatat gaacttgcag gatgtgaaga 8640atatctacct
agctatgtgc ttaactgttg ccatgacctt gtggcgacgc aggatggtgc
8700tttcacaaaa cgcggtggcc tgtcgtctgg agacccagtc accagtgtgt
ccaacactgt 8760gtactcactg gtgatttatg cccagcacat ggtactatct
gccctgaaaa tgggtcatga 8820aattggcctc aagttcctcg aagaacaact
caaatttgag gaccttcttg aaatccagcc 8880tatgttagta tactctgatg
atcttgtctt gtacgcagaa aagcccacct tccccaacta 8940tcattggtgg
gtcgagcatc ttgacctgat gttgggcttt aaaacggacc caaagaaaac
9000cgtcataact gataaaccca gtttcctcgg gtgcagaatc gaagcagggc
gacagctagt 9060ccccaatcgc gaccgcatcc tggctgctct tgcatatcac
atgaaggcgc agaacgcctc 9120agagtattat gcgtctgctg ccgcaatcct
gatggattca tgtgcttgca ttgaccacga 9180tcctgaatgg tatgaggacc
tcatctgtgg cattgcccga tgcgctcgcc tggacggtta 9240tagctttcca
ggtccggcat ttttcatgtc catgtgggag aagctgagga gtcataatga
9300agggaagaaa ttccgccact gcggcatctg cgacgccaaa gccgactacg
cggctgcttg 9360tgggcttgat ttgtgtttgt ttcattcgca ctttcaccaa
cactgccctg tcactctgag 9420ctgcggtcac catgccggtt cgaaggaatg
ttcgcagtgt cagtcacctg ttggggccgg 9480cagatcccct cttgatgctg
tgctggaaca aattccatac aaacctcctc gcactgtcat 9540catgaaggtg
ggtaataaaa caacggccct tgatccgggg aggtaccagt cccgtcgagg
9600tctcgttgca gtcaagaggg gtattgcggg taatgaagtt gatcttgctg
atggagatta 9660ccaagtggta cctcttctgc cgacttgcaa agatataaac
atggtgaagg tggcttgcaa 9720cgtactactc agcaagttca tagtagggcc
accaggttcc gggaagacca cctggctact 9780aagtcaagtt caggacgatg
atgtcattta cacacccact catcagacca tgtttgacat 9840agttagtgct
ctcaaagttt gcaggtattc cattccagga gcctcgggac tccctttccc
9900accgcctgcc aggtccgggc cgtgggttag gctcattgcc agcgggcacg
tccctggccg 9960agtatcatac ctcgatgagg ccggatattg caatcatctg
gacattctta gactgctttc 10020caaaacaccc cttgtgtgtt tgggtgacct
tcagcaactt cacccagtcg gctttgattc 10080ctattgttat gtgttcgatc
agatgcctca gaagcagttg accaccattt acagatttgg 10140ccccaacatc
tgcgcagcca tccagccttg ttacagggag aaacttgaat ctaaggctag
10200gaacaccagg gtggttttta ccacccggcc tgtggccttt ggccaggtgc
tgacaccata 10260tcacaaagat cgcgtcggct ccgcgattac catagactca
tcccaggggg ccacctttga 10320cattgtaaca ttgcatctac catcgccaaa
gtccctaaat aagtcccggg cacttgtggc 10380catcacacgg gcaagacacg
ggttgttcat ttatgaccct cacaaccagc tccgggagtt 10440tttcaaccta
acccctgagc gcactgattg taaccttgtg ttcagccgtg gagatgagct
10500ggtggtcctg aatgcagata atgcagtcac aaccgtggcg aaggccttag
agacaggtcc 10560aactcaattt cgagtgtcag acccgaggtg caagtctctc
ttagccgctt gctcggccag 10620tctggaaggg agctgcatgc cgctaccgca
agtggcgcat aacctggggt tttacttctc 10680cccagacagt ccagtatttg
cacctctgcc aaaagagttg gcgccacatt ggccagtggt 10740tacccatcag
aataatcggg cgtggcctga tcgacttgtc gctagtatgc gtccaattga
10800cgcccgctac agcaagccga tggtcggtgc agggtatgtg gtcggaccgt
ccaccttcct 10860tggtactcct ggagtggtgt catactatct cacactatac
atcaggggtg agccccaggc 10920cttgccagaa acacttgttt caacaggacg
tatagccaca gattgtcggg agtatctcga 10980cgcggctgag gaagaggcag
caaaagaact tccccacgcg ttcattggcg atgtcaaagg 11040caccacagtt
ggggggtgtc atcacattac atcaaaatac ctacctaggt ccctgcctaa
11100agactctgtt gccgtagttg gagtaagttc gcctggcagg gctgctaaag
ccgtatgcac 11160cctcaccgat gtgtacctcc ctgaactccg gccatatctg
caacctgaga cggcatcaaa 11220atgctggaaa ctcaaattag acttcaggga
cgtccgacta atggtctgga aaggagccac 11280cgcctacttt caattggaag
ggctcacatg gtcggcgctg cctgactatg ccaggtttat 11340tcagctgccc
aaaaacgctg ttgtatacat cgatccgtgc ataggaccgg caacagccaa
11400tcgtaaagtc gtacgaacca cagattggcg ggccgacctg gcagtgacgc
cgtatgatta 11460cggtgcccgg aacattttga caacagcctg gttcgaggac
ctcgggccgc agtggaagat 11520tctggggttg cagcccttta ggcgggcgtt
tggctttgaa aacactgagg attgggcaat 11580ccttgcatgc tgcatgagtg
acggcaagga ctacactgac tataactgga attgcgttcg 11640acaacgccca
cacgctatcc atggacgcgc tcgtgaccat acgtaccact ttgcccctgg
11700cactgaattg caagtggagc tcggtaaacc ccggctgcca cctgagcaag
taccgtgaat 11760tcggagtgat gcaatggggt cactgtggag taaaatcagc
cagctgttcg tggacgcctt 11820cactgaattc cttgttagtg tggttgatat
tgtcatcttc cttgccatat tgtttgggtt 11880caccgtcgca ggatggttac
tggtctttct tctcagagtg gtttgctccg cgcttctccg 11940ttcgcgctct
gccattcact cttccgaact atcgaaggtc ctatgagggc ttactaccta
12000attgcagacc ggatgttcca caatttgcat ttaagcaccc tttgggtatg
ttttggcaca 12060tgcgggtttc ccacctaatt gatcagatgg tctctcgccg
catctaccag accatggaac 12120attcaggtca agcggcctgg aagcacgtgg
tcagtgaggc tactcttaca aaattgtcag 12180aactcgacat agttctccac
ttccaacacc tggccgcagt ggaggcggac tcttgtcgct 12240tcctcagctc
acgacttgtg atgctgaaaa atcttgccgt tggcaatgtg agcttgcagt
12300acaacaccac gttgaaccgc gttgagctca tcctccccac accaggtacg
aggcccaaat 12360tgaccgattt cagacaatgg ctcatcagtg tgcacgcttc
cattttttcc tctgtagcct 12420catcagttac tttgttcata gtgctttggc
ttcgaattcc agccgtacgc tatgtttttg 12480gtttccattg gcccatggca
acacgtcatt cgagctgacc attaattaca ctatatgcat 12540gccctgtctt
accagccaag cggctcaaca aaggctcgaa cccggtcgta acatgtggtg
12600caaaatagga cacaccacgt gcgaggagcg tgaccatgat gagttgtcaa
tgtccatccc 12660gtccgggtac gacaacctca aacttgaagg ttattacgct
tggctggctt ttttgtcctt 12720ttcctacgcg gcccaattcc atccggagtt
gtttggaata gggaatgtgt cgcgcgtctt 12780tgtggataaa cgacaccagt
tcatttgtgc cgagcatgac ggagataatt caaccgtatc 12840taccggacac
aacatctccg catcatatgc ggcatattat caccaccaaa tagacggggg
12900caattggttc catttggaat ggctgcggcc gctcttttcc tcttggctag
tgctcaacat 12960atcatggttt ctgaggcgtt cgcctgcaag ccctgtttct
cgacgcatct atcagatatt 13020aagaccaata cgaccgcggc tgccggtttc
atggtccttc aagacatcag ttgcctccga 13080cctcacaggg tctcagcatc
gcaagagaac attcccttcg gaaagtcgtc acaatgtcgt 13140gaagccgtcg
gtactcccca gtacattacg atgactgcta atgtgaccga cgaatcatat
13200ttgtacaacg cggacttgct aatgctttcc gcgtgccttt tccacgcctc
agaaatgagc 13260gagaaaggct tcaaagttat ctttggaaac gtctccggcg
ttgtttcagc ttgtgtcaat 13320ttcacagatt atgtggccca tgtaacccaa
catacccaac agcatcatct ggtaattgat 13380cacattcggt tactgcattt
cctgacacca tctgcaatga ggtgggctac aaccattgct 13440tgtttgttcg
ccattctctt agcgatatga gatgttctca caaattgggg cgtttcttga
13500ctccgcactc ttgcttctgg tggctttttt tgctgtgtac cggcttgtcc
tggtcctttg 13560ccgatggcaa cggcaacagc tcgacacgcc aatacatata
taacttgacg atatgcgagc 13620tgaatgggac cgtctggttg tccagtcatt
ttgattgggc agtcgagacc tttgtgcttt 13680acccggtggc cactcatatc
ctctcactgg gttttctcac aacaagccat ttttttgatg 13740cgctcggtct
cggcgctgtg tccactacgg gatttcttgg cgggcggtat gtacttagca
13800gcgtgtacgg cgcctgcgcc ttcgcagcgc ttgtatgttt tgtcatccgt
gctgctaaaa 13860attgcatggc ttgccgttat gcccgcaccc ggttcaccaa
cttcatcgtg gacgaccggg 13920ggaagatcca tcgatggaag tccccaatag
tggtagagaa attaggcaaa gctgacatcg 13980gcggcgacct tgtcaccatc
aaacatgttg tcctcgaagg agtcaaagct caacctttga 14040cgaggacatc
ggcggagcaa tgggaagcct agatgatttt tgcaatgatc ctaccgccgc
14100acagaagctt gtgctggcat ttagtatcac atacacacct ataatgatat
acgccctcaa 14160ggtgtcacgc ggccggctcc taggactgtt acacatcctg
atatttctga actgttcttt 14220cacgttcgga tacatgacat acgtgcactt
tcaatccact aaccgtgtcg cgcttactat 14280gggggcggtc gttgcccttt
tgtggggcat ttacagcttt atagaatcat ggaagtttgt 14340cacttccaga
tgcaggttgt gttgcctagg ccggcgatac attctggccc ctgcccacca
14400cgtagaaagt gctgcaggcc tccattcaat cccagcgtct ggtaaccgag
catacgctgt 14460gagaaagccc ggactaacat cagtgaacgg cactctagta
ccaggacttc ggagcctcgt 14520gttgggcggc aaacgagctg ttaaacgagg
agtggttaac ctcgtcaagt atggccggta 14580aaaaccagag ccagaagaaa
aagaaaaaca cagctcctat ggggagtggc cagccagtca 14640atcaactgtg
ccaattgctg ggcacaatga taaagtccca gcgccagcgg cctaggggag
14700gacaggccaa aatgaaaaag cctgagaagc cacatttccc cctagctgct
gaagatgaca 14760tccggcacca tttcacccag accgagcgtt ccctttgctt
gcaatcgatc cagacggcct 14820tcaatcaagg cgcaggaact gcgtcgcttt
catccagcgg gaaggtcagt tttcaggttg 14880agttcatgct gccggtcgct
catacggtgc gcctgattcg cgtaacttcc acatccgcca 14940gtcagggtgc
aagttaattt gatagttagg tgaatggccg cgattggcgt gtggcctctg
15000agtcacctat tcaattaggg cgatcacatg ggggttagac ttaattggcg
agaaccatgt 15060gaccgaaatt aaaaaaaaaa aaaaaaaaaa aaaaaaaa
150983650PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 36Ser Xaa Xaa Tyr Xaa Xaa Xaa
Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10 15 Xaa Xaa Gly Xaa Xaa
Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa 20 25 30 Leu Glu Xaa
Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 35 40 45 Arg
Ser 50 3749PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 37Ser Xaa Xaa Tyr Xaa Xaa Xaa
Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10 15 Xaa Xaa Gly Xaa Met
Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu 20 25 30 Glu Xaa Leu
Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg 35 40 45 Ser
3848PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 38Ser Xaa Xaa Tyr Xaa Xaa Xaa
Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Met Trp
Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu 20 25 30 Xaa Leu Pro
Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 45
3947PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 39Ser Xaa Xaa Tyr Xaa Xaa Xaa
Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10 15 Xaa Xaa Met Trp Xaa
Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu Xaa 20 25 30 Leu Pro Xaa
Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 45
4046PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(3)Any amino acid 40Ser Xaa Xaa Tyr Xaa Xaa Xaa
Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa 1 5 10 15 Xaa Met Trp Xaa Xaa
Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu Xaa Leu 20 25 30 Pro Xaa Xaa
Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 45 4145PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(2)..(3)Any amino
acid 41Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa
Xaa 1 5 10 15 Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu
Xaa Leu Pro 20 25 30 Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa
Arg Ser 35 40 45 4246PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(2)..(2)Any amino acid 42Ala Xaa Val Tyr Asp
Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5 10 15 Xaa Xaa Xaa
Ser Trp Ala Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa 20 25 30 Xaa
Xaa Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 40 45
4345PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 43Ala Xaa Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5 10 15 Xaa Xaa Xaa Ser Trp
Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa 20 25 30 Xaa Xaa Leu
Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 40 45 4444PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(2)..(2)Any amino
acid 44Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala
Xaa 1 5 10 15 Xaa Xaa Xaa Ser Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa
Xaa Xaa Xaa 20 25 30 Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa
Ser 35 40 4543PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 45Ala Xaa Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5 10 15 Xaa Xaa Xaa Gly Gly
Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30 Leu Xaa Leu
Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 40 4642PRTPorcine reproductive
and respiratory syndrome virusMOD_RES(2)..(2)Any amino acid 46Ala
Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5 10
15 Xaa Xaa Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa Leu
20 25 30 Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 40
4741PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 47Ala Xaa Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Xaa Val Ala Xaa 1 5 10 15 Xaa Gly Gly Xaa Xaa
Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 20 25 30 Leu Xaa Ala
Xaa Xaa Xaa Xaa Xaa Ser 35 40 4850PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(17)..(17)Any amino acid 48Ser Ser
Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15
Xaa Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa 20
25 30 Leu Glu Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu
Ile 35 40 45 Arg Ser 50 4949PRTPorcine reproductive and respiratory
syndrome virusMOD_RES(17)..(17)Any amino acid 49Ser Ser Val Tyr Arg
Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Xaa Asn Gly
Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu 20 25 30 Glu
Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg 35 40
45 Ser 5048PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(17)..(17)Any amino acid 50Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Xaa Asn Met Met Trp
Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu 20 25 30 Xaa Leu Pro
Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 45
5147PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(17)..(17)Any amino acid 51Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Xaa Met Met
Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu Xaa 20 25 30 Leu
Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 45
5246PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(28)..(28)Any amino acid 52Ser Ser Val Tyr Arg Trp Lys
Lys Phe Val Val Phe Thr Asp Ser Ser 1 5 10 15 Met Met Trp Thr Pro
Glu Ser Asp Asp Ser Ala Xaa Leu Glu Xaa Leu 20 25 30 Pro Pro Glu
Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 45 5345PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(27)..(27)Any
amino acid 53Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr
Asp Ser Met 1 5 10 15 Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa
Leu Glu Xaa Leu Pro 20 25 30 Pro Glu Leu Glu Arg Gln Val Glu Ile
Leu Ile Arg Ser 35 40 45 5446PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(8)..(9)Any amino acid 54Ala Thr
Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5 10 15
Xaa Lys Val Ser Trp Ala Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val 20
25 30 Pro Xaa Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40
45 5545PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(8)..(9)Any amino acid 55Ala Thr Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5 10 15 Xaa Lys Val Ser Trp
Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro 20 25 30 Xaa Glu Leu
Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40 45 5644PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(8)..(9)Any amino
acid 56Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala
Xaa 1 5 10 15 Xaa Lys Val Ser Gly Gly Xaa Glu Val Lys Phe Glu Xaa
Val Pro Xaa 20 25 30 Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr
Ser 35 40 5743PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(8)..(9)Any amino acid 57Ala Thr Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5 10 15 Xaa Lys Val Gly Gly
Xaa Glu Val Lys Phe Glu Xaa Val Pro Xaa Glu 20 25 30 Leu Lys Leu
Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40 5842PRTPorcine reproductive
and respiratory syndrome virusMOD_RES(8)..(9)Any amino acid 58Ala
Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5 10
15 Xaa Lys Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro Xaa Glu Leu
20 25 30 Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40
5941PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(8)..(9)Any amino acid 59Ala Thr Val Tyr Asp Ile Gly
Xaa Xaa Ala Val Met Tyr Val Ala Xaa 1 5 10 15 Xaa Gly Gly Xaa Glu
Val Lys Phe Glu Xaa Val Pro Xaa Glu Leu Lys 20 25 30 Leu Xaa Ala
Asn Arg Leu Xaa Thr Ser 35 40 604PRTPorcine reproductive and
respiratory syndrome virusMOD_RES(2)..(3)Any amino acid 60Ser Xaa
Xaa Tyr 1 614PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 61Ala Xaa Val Tyr 1
623PRTPorcine reproductive and respiratory syndrome virus 62Ser Phe
Pro 1 634PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 63Arg Xaa Met Trp 1
643PRTPorcine reproductive and respiratory syndrome virus 64Arg Gly
Gly 1 653PRTPorcine reproductive and respiratory syndrome virus
65Arg Met Met 1 663PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(2)..(2)Any amino acid 66Arg Xaa Arg 1 673PRTPorcine
reproductive and respiratory syndrome virus 67Ala Pro Arg 1
684PRTPorcine reproductive and respiratory syndrome
virusMOD_RES(3)..(3)Any amino acid 68Gly Arg Xaa Arg 1
694PRTPorcine reproductive and respiratory syndrome virus 69Trp Ala
Pro Arg 1 703PRTPorcine reproductive and respiratory syndrome virus
70Trp Tyr Gly 1 713PRTPorcine reproductive and respiratory syndrome
virus 71Ala Gly Arg 1 723PRTPorcine reproductive and respiratory
syndrome virus 72Ala Ala Gly 1 734PRTPorcine reproductive and
respiratory syndrome virus 73Glu Cys Ala Met 1 745PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(2)..(2)Any amino
acid 74Ala Xaa Val Tyr Asp 1 5 754PRTPorcine reproductive and
respiratory syndrome virus 75Glu Glu Ala His 1 765PRTPorcine
reproductive and respiratory syndrome virusMOD_RES(2)..(2)Any amino
acid 76Ser Xaa Val Tyr Arg 1 5 772PRTPorcine reproductive and
respiratory syndrome virus 77Pro Arg 1 783PRTPorcine reproductive
and respiratory syndrome virus 78Arg Pro Arg 1 794PRTPorcine
reproductive and respiratory syndrome virus 79Gly Arg Pro Arg 1
805PRTPorcine reproductive and respiratory syndrome virus 80Asn Gly
Arg Pro Arg 1 5 816PRTPorcine reproductive and respiratory syndrome
virus 81Pro Asn Gly Arg Pro Arg 1 5 82389PRTPorcine reproductive
and respiratory syndrome virus 82Met Ser Gly Thr Phe Ser Arg Cys
Met Cys Thr Pro Ala Ala Arg Val 1 5 10 15 Phe Trp Asn Ala Gly Gln
Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30 Ser Leu Leu Pro
Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45 Leu Phe
Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60
Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly 65
70 75 80 Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe
Leu Gln 85 90 95 Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp
Gly Cys Leu Thr 100 105 110 Ser Arg His Leu Arg Glu Leu Gln Val Tyr
Glu Arg Gly Cys Ser Trp 115 120 125 Tyr Pro Ile Thr Gly Pro Val Pro
Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140 Met His Val Ser Asp Gln
Pro Phe Pro Gly Ala Thr His Val Leu Thr 145 150 155 160 Asn Ser Pro
Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175 Glu
Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185
190 Thr Asp Ser Ser Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu
195 200 205 Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu
Glu Arg 210 215 220 Gln Val Glu Ile Leu Ile Arg Ser Phe Pro Ala His
His Pro Val Ser 225 230 235 240 Leu Ala Asp Trp Glu Leu Ala Glu Ser
Pro Glu Asn Gly Phe Ser Phe 245 250 255 Ser Thr Tyr His Ser Gly Gly
Tyr Leu Val Gln Asn Pro Asp Val Phe 260 265 270 Asp Ser Lys Cys Trp
Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val 275 280 285 Arg Leu Tyr
Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys 290 295 300 Trp
Gly Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly 305 310
315 320 Ile Arg Ala Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala
Leu 325 330 335 Ser Cys Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp
Asp Asp Val 340 345 350 Thr Pro Gly Phe Val Arg Leu Thr Ser Leu Arg
Ile Val Pro Asn Thr 355 360 365 Glu Pro Thr Thr Leu Arg Ile Phe Arg
Phe Gly Ala His Lys Trp Tyr 370 375 380 Gly Ala Ala Gly Lys 385
83203PRTPorcine reproductive and respiratory syndrome virus 83Ala
Asp Val Tyr Asp Ile Gly Arg Gly Ala Val Met Tyr Val Ala Gly 1 5 10
15 Gly Lys Val Ser Trp Ala Pro Arg Gly Gly Asn Glu Val Lys Phe Glu
20 25 30 Pro Val Pro Lys Glu Leu Lys Leu Val Ala Asn Arg Leu His
Thr Ser 35 40 45 Phe Pro Pro His His Val Val Asp Met Ser Lys Phe
Thr Phe Ile Thr 50 55 60 Pro Gly Ser Gly Val Ser Met Arg Val Glu
Tyr Gln Tyr Gly Cys Leu 65 70 75 80 Pro Ala Asp Thr Val Pro Glu Gly
Asn Cys Trp Trp Arg Leu Leu Asp 85 90 95 Ser Leu Pro Pro Glu Val
Gln Tyr Lys Glu Ile Arg His Ala Asn Gln 100 105 110 Phe Gly Tyr Gln
Thr Lys His Gly Val Pro Gly Lys Tyr Leu Gln Arg 115 120 125 Arg Leu
Gln Val Asn Gly Leu Arg Ala Val Thr Asp Thr His Gly Pro 130 135 140
Ile Val Ile Gln Tyr Phe Ser Val Lys Glu Ser Trp Ile Arg His Leu 145
150 155 160 Lys Leu Val Glu Glu Pro Ser Leu Pro Gly Phe Glu Asp Leu
Leu Arg 165 170 175 Ile Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Gly
Lys Asp Glu Lys 180 185 190 Ile Phe Arg Phe Gly Ser His Lys Trp Tyr
Gly 195 200 84203PRTPorcine reproductive and respiratory syndrome
virus 84Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val Met Tyr Val Ala
Glu 1 5 10 15 Arg Lys Val Ser Trp Ala Pro Arg Gly Gly Asp Glu Val
Lys Phe Glu 20 25 30 Ala Val Pro Gly Glu Leu Lys Leu Ile Ala Asn
Arg Leu Arg Thr Ser 35 40 45 Phe Pro Pro His His Thr Val Asp Met
Ser Lys Phe Ala Phe Thr Ala 50 55 60 Pro Gly Cys Gly Val Ser Met
Arg Val Glu Arg Gln His Gly Cys Leu 65 70 75 80 Pro Ala Asp Thr Val
Pro Glu Gly Asn Cys Trp Trp Ser Leu Phe Asp 85 90 95 Leu Leu Pro
Leu Glu Val Gln Asn Lys Glu Ile Arg His Ala Asn Gln 100 105 110 Phe
Gly Tyr Gln Thr Lys His Gly Val Ser Gly Lys Tyr Leu Gln Arg 115 120
125 Arg Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp Leu Asn Gly Pro
130 135 140 Ile Val Val Gln Tyr Phe Ser Val Lys Glu Ser Trp Ile Arg
His Leu 145 150 155 160 Lys Leu Ala Gly Glu Pro Ser Tyr Ser Gly Phe
Glu Asp Leu Leu Arg 165 170 175 Ile Arg Val Glu Pro Asn Thr Ser Pro
Leu Ala Asp Lys Glu Glu Lys 180 185 190 Ile Phe Arg Phe Gly Ser His
Lys Trp Tyr Gly 195 200
* * * * *