U.S. patent application number 14/720505 was filed with the patent office on 2015-12-03 for antibody formulations and methods.
This patent application is currently assigned to PROTHENA THERAPEUTICS LIMITED. The applicant listed for this patent is PROTHENA THERAPEUTICS LIMITED. Invention is credited to Patrick GARIDEL, Isaac Craig HENDERSON, Pamela KLEIN.
Application Number | 20150344555 14/720505 |
Document ID | / |
Family ID | 48168501 |
Filed Date | 2015-12-03 |
United States Patent
Application |
20150344555 |
Kind Code |
A1 |
GARIDEL; Patrick ; et
al. |
December 3, 2015 |
ANTIBODY FORMULATIONS AND METHODS
Abstract
Antibody formulations and methods useful for prophylaxis or
treatment of amyloidosis, including AA amyloidosis and AL
amyloidosis.
Inventors: |
GARIDEL; Patrick; (Ingelheim
am Rhein, DE) ; HENDERSON; Isaac Craig; (San
Francisco, CA) ; KLEIN; Pamela; (San Mateo,
CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
PROTHENA THERAPEUTICS LIMITED |
Dublin |
|
IE |
|
|
Assignee: |
PROTHENA THERAPEUTICS
LIMITED
DUBLIN
IE
|
Family ID: |
48168501 |
Appl. No.: |
14/720505 |
Filed: |
May 22, 2015 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
13660957 |
Oct 25, 2012 |
9089529 |
|
|
14720505 |
|
|
|
|
61551406 |
Oct 25, 2011 |
|
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Current U.S.
Class: |
435/69.6 ;
435/320.1; 435/328; 536/23.53 |
Current CPC
Class: |
A61P 35/00 20180101;
C07K 2317/76 20130101; A61P 25/00 20180101; A61P 19/02 20180101;
A61P 31/08 20180101; A61P 31/00 20180101; A61P 11/08 20180101; A61P
29/00 20180101; C07K 2317/24 20130101; A61P 13/02 20180101; A61K
39/3955 20130101; A61P 25/28 20180101; A61K 31/198 20130101; A61P
31/06 20180101; A61P 17/06 20180101; A61P 13/12 20180101; A61P
17/00 20180101; A61K 9/19 20130101; A61K 31/195 20130101; A61P 7/00
20180101; C07K 2317/41 20130101; A61K 47/22 20130101; C07K 2317/56
20130101; A61P 1/04 20180101; A61P 31/04 20180101; A61K 31/454
20130101; A61P 43/00 20180101; A61K 31/69 20130101; A61K 38/07
20130101; C07K 2317/14 20130101; A61K 2039/545 20130101; C07K 16/18
20130101; A61K 47/26 20130101; A61K 2039/505 20130101; C07K
2317/565 20130101; A61K 39/39591 20130101 |
International
Class: |
C07K 16/18 20060101
C07K016/18 |
Claims
1-33. (canceled)
34. A nucleic acid comprising a nucleotide sequence encoding at
least one polypeptide having an amino acid sequence set forth as
any one of SEQ ID NOs: 13-16.
35. The nucleic acid of claim 34 comprising the nucleotide sequence
of SEQ ID NO: 19.
36. The nucleic acid of claim 35 comprising the nucleotide sequence
of SEQ ID NO: 20.
37-38. (canceled)
39. The nucleic acid of claim 38 comprising the nucleotide sequence
of SEQ ID NO: 22.
40. The nucleic acid of claim 39 comprising the nucleotide sequence
of SEQ ID NO: 23.
41. A vector comprising at least one nucleic acid of claim 34.
42. The vector of claim 41, comprising a nucleic acid comprising a
nucleotide sequence encoding a polypeptide having an amino acid
sequence set forth as SEQ ID NO: 13 and a nucleic acid comprising a
nucleotide sequence encoding a polypeptide having an amino acid
sequence set forth as SEQ ID NO: 15.
43. The vector of claim 41, comprising a nucleic acid comprising a
nucleotide sequence set forth as SEQ ID NO: 19 and a nucleic acid
comprising a nucleotide sequence set forth as SEQ ID NO: 22.
44. The vector of claim 41, comprising a nucleic acid comprising a
nucleotide sequence set forth as SEQ ID NO: 20 and a nucleic acid
comprising a nucleotide sequence set forth as SEQ ID NO: 23.
45. A host cell comprising at least one nucleic acid of claim 34
stably incorporated into its genome.
46. The host cell of claim 45, wherein the host cell is a CHO
cell.
47. The host cell of claim 45 having stably incorporated into its
genome a nucleic acid comprising a nucleotide sequence encoding a
polypeptide having an amino acid sequence set forth as SEQ ID NO:
13 and a nucleic acid comprising a nucleotide sequence encoding a
polypeptide having an amino acid sequence set forth as SEQ ID NO:
15.
48. The host cell of claim 45 having stably incorporated into its
genome a nucleic acid comprising a nucleotide sequence set forth as
SEQ ID NO: 19 and a nucleic acid comprising a nucleotide sequence
set forth as SEQ ID NO: 22.
49. The host cell of claim 45 having stably incorporated into its
genome a nucleic acid comprising a nucleotide sequence set forth as
SEQ ID NO: 20 and a nucleic acid comprising a nucleotide sequence
set forth as SEQ ID NO: 23.
50. The host cell of claim 47, wherein the host cell is a CHO
cell.
51. A method of making a pharmaceutical formulation comprising: (a)
culturing mammalian cells having stably incorporated into their
genome one or more nucleic acids encoding a light chain and a heavy
chain of a humanized 2A4 antibody so that the mammalian cells
secrete the antibody into cell culture media; (b) purifying the
antibody from the cell culture media; and (c) preparing the
pharmaceutical formulation.
52. The method of claim 51, wherein the nucleic acid encoding the
light chain of a humanized 2A4 antibody comprises a nucleotide
sequence encoding a polypeptide having an amino acid sequence set
forth as SEQ ID NO: 13, and wherein the nucleic acid encoding the
heavy chain of a humanized 2A4 antibody comprises a nucleotide
sequence encoding a polypeptide having an amino acid sequence set
forth as any one of SEQ ID NOs: 14-16.
53. The method of claim 52, wherein the nucleic acid encoding the
light chain of a humanized 2A4 antibody comprises a nucleotide
sequence encoding a polypeptide having an amino acid sequence set
forth as SEQ ID NO: 13, and wherein the nucleic acid encoding the
heavy chain of a humanized 2A4 antibody comprises a nucleotide
sequence encoding a polypeptide having an amino acid sequence set
forth as SEQ ID NO: 15.
54. The method of claim 53, wherein the nucleic acid encoding the
light chain of a humanized 2A4 antibody comprises a nucleotide
sequence of SEQ ID NO: 19, and wherein the nucleic acid encoding
the heavy chain of a humanized 2A4 antibody comprises a nucleotide
sequence of SEQ ID NO: 22.
55. The method of claim 54, wherein the nucleic acid encoding the
light chain of a humanized 2A4 antibody comprises a nucleotide
sequence of SEQ ID NO: 20, and wherein the nucleic acid encoding
the heavy chain of a humanized 2A4 antibody comprises a nucleotide
sequence of SEQ ID NO: 23.
56-86. (canceled)
Description
RELATED APPLICATIONS
[0001] Priority is claimed to U.S. Provisional Application No.
61/551,406, filed 25 Oct. 2011, which is incorporated by reference
herein in its entirety.
TECHNICAL FIELD
[0002] The invention resides in the technical fields of immunology
and medicine.
BACKGROUND OF THE INVENTION
[0003] Amyloidosis is a general term that describes a number of
diseases characterized by the existence of pathological forms of
amyloid proteins, often involving extracellular deposition of
protein fibrils, which form numerous "amyloid deposits" or "amyloid
plaques," which may occur in local sites or systematically. These
deposits or plaques are composed primarily of a naturally occurring
soluble protein or peptide, assembled into extensive insoluble
deposits 10-100 .mu.m in diameter in a variety of tissue sites. The
deposits are composed of generally lateral aggregates of fibrils
that are approximately 10-15 nm in diameter. Amyloid fibrils
produce a characteristic apple green birefringence in polarized
light, when stained with Congo Red dye. Generally, the fibrillar
composition of these deposits is an identifying characteristic for
the various forms of amyloid disease.
[0004] The peptides or proteins forming the plaque deposits are
often produced from a larger precursor protein. More specifically,
the pathogenesis of amyloid aggregates such as fibril deposits
generally involves proteolytic cleavage of an "abnormal" precursor
protein into fragments that aggregate into anti-parallel .beta.
pleated sheets.
[0005] Systemic amyloidoses are a complex group of diseases caused
by tissue deposition of misfolded proteins that result in
progressive organ damage. The most common type, AL amyloidosis or
primary amyloidosis, involves a hematological disorder caused by
clonal plasma cells that produce misfolded immunoglobulin light
chains. Overproduction of misfolded light chain by plasma cells
results in deposits of abnormal AL protein (amyloid), in the
tissues and organs of individuals with AL amyloidosis. Clinical
features of AL amyloidosis include a constellation of symptoms and
organ dysfunction that can include cardiac, renal, and hepatic
dysfunction, gastrointestinal involvement, neuropathies and
macroglossia. The mechanisms by which amyloidogenic immunoglobulin
light chains result in organ dysfunction are not well
characterized, however, it is hypothesized that both amyloid
deposits and prefibrillar aggregates may contribute to cytotoxic
effects on organs observed in patients with AL amyloidosis. AL
amyloidosis is a disease entity of its own, although AL amyloidosis
can occur concurrently in a small subset (up to 15%) of patients
with multiple myeloma.
[0006] AL amyloidosis is a rare disorder with an estimated
incidence of 8 in 1,000,000 people. Only 1200 to 3200 new cases of
AL amyloidosis are reported each year in the United States. Two
thirds of patients with AL amyloidosis are male and less than 5% of
patients are under 40 years of age. Both the causes and origins of
AL amyloidosis remain poorly understood.
[0007] Current treatment of patients with AL amyloidosis is aimed
at reducing or eliminating the bone marrow disorder, i.e. the
plasma cells that are responsible for producing the light chains,
thereby limiting or halting the production of amyloid. The most
aggressive treatment options include stem cell transplant and
high-dose chemotherapy for those patients who can tolerate it.
Other treatment regimens include combinations of drugs often used
to treat hematological malignancies, such as melphalan, prednisone,
dexamethasone and proteosome inhibitors such as bortezomib, in an
attempt to reduce light chain production. There are no currently
approved treatments for AL amyloidosis that directly target
potentially toxic forms of the amyloidogenic proteins.
[0008] A different form of systemic amyloidosis, AA amyloidosis or
secondary amyloidosis, occurs "secondarily" as a result of other
illness, such as chronic inflammatory diseases (for example,
rheumatoid arthritis and ankylosing spondylitis) or chronic
infections (for example, tuberculosis or osteomyelitis). In
secondary amyloidosis, the depositing amyloid protein is amyloid A
protein, derived from an acute-phase protein serum amyloid A. The
treatment of secondary amyloidosis is directed at treating the
underlying illness.
[0009] Thus, there is a need for therapies to treat AA amyloidosis
and AL amyloidosis, which directly target the pathological amyloid
fibrils. The present invention provides pharmaceutical formulations
of 2A4 and 7D8 antibodies, and chimeric and humanized versions
thereof, which show high affinity binding to both AL and AA
amyloids due to a shared immunogenic epitope of the pathological
forms of these proteins.
SUMMARY OF THE INVENTION
[0010] The present invention provides antibody formulations useful
for prophylaxis and treatment of amyloid disease. In one aspect of
the invention, a pharmaceutical formulation comprises (a) a
chimeric or humanized version of antibody 2A4 (ATCC Accession
Number PTA-9662) or of antibody 7D8 (ATCC Accession Number
PTA-9468), or fragment thereof, which specifically competes for
binding to antigen with 2A4 or 7D8, and/or which is directed to an
epitope comprising AEDS (SEQ ID NO: 18), wherein the antibody is
present at a concentration within the range from about 1 mg/mL to
about 100 mg/mL; (b) histidine buffer present at a concentration
within the range from about 20 mM to about 30 mM; (c) trehalose
present at a concentration within the range from about 210 mM to
about 250 mM; and (d) polysorbate 20 present at a concentration
within the range from about 0.005% to about 0.05% by weight;
wherein the formulation is characterized by a pH within the range
from about 6 to about 7. For example, representative formulations
of the invention comprise an antibody having a light chain variable
region comprising an amino acid sequence set forth as SEQ ID NO: 4
and/or a heavy chain variable region comprising an amino acid
sequence set forth as SEQ ID NO: 5. More particularly, such a
formulation can comprise an antibody having a light chain
comprising an amino acid sequence set forth as SEQ ID NO: 13 and a
heavy chain comprising an amino acid sequence set forth as any one
of SEQ ID NO: 14-16, for example, an antibody having a light chain
comprising an amino acid sequence set forth as SEQ ID NO: 13 and a
heavy chain comprising an amino acid sequence set forth as SEQ ID
NO: 15.
[0011] Additional representative formulations of the invention
comprise (a) an antibody having a light chain variable region
comprising three complementarity determining regions set forth as
SEQ ID NOs: 6, 7, and 8, and a heavy chain variable region
comprising three complementarity regions set forth as SEQ ID NOs:
9, 10, and 11; and (b) an antibody having a light chain variable
region comprising three complementarity determining regions set
forth as SEQ ID NOs: 12, 7, and 8, and a heavy chain variable
region comprising three complementarity regions set forth as SEQ ID
NOs: 9, 10, and 11.
[0012] In representative formulations of the invention, the
antibody is present at a concentration within the range from about
5 mg/mL to about 15 mg/mL (e.g., about 10 mg/mL), or present at a
concentration within the range from about 25-75 mg/mL (e.g., 50
mg/mL).
[0013] In other representative formulations of the invention,
histidine buffer is present at a concentration of about 25 mM. The
histidine buffer can comprise L-histidine and L-histidine HCl
monohydrate. For example, L-histidine can be used at a
concentration within the range from about 16 mM to about 22 mM and
L-histidine HCl monohydrate can be used at a concentration within
the range from about 4 mM to about 8 mM.
[0014] In other representative formulations of the invention,
trehalose is present at a concentration of about 230 mM.
[0015] Prepared as described herein, representative formulations of
the invention (a) are characterized by an osmolality of about 300
mOsm/kg; (b) comprise less than about 10% of the antibody present
as an aggregate in the formulation; (c) further comprise a bulking
agent; (d) are sterile; and/or (e) are stable upon freezing and
thawing.
[0016] In one aspect of the invention, a formulation comprises (a)
an antibody comprising a light chain comprising an amino acid
sequence set forth as SEQ ID NO: 13 and a heavy chain comprising an
amino acid sequence set forth as any one of SEQ ID NOs: 14-16, and
which is present at a concentration of about 10 mg/mL; (b) a
histidine buffer present at a concentration of about 25 mM; (c)
trehalose present at a concentration of about 230 mM; (d)
polysorbate 20 present at a concentration of about 0.2 g/L; and (e)
a pH of about 6.5.
[0017] In another aspect of the invention, a pharmaceutical
formulation comprises (a) an antibody, which is antibody 2A4 (ATCC
Accession Number PTA-9662), antibody 7D8 (ATCC Accession Number
PTA-9468), or a chimeric or humanized version of antibody 2A4 or of
antibody 7D8, or fragment thereof, which specifically competes for
binding to antigen with 2A4 or 7D8, and/or which is directed to an
epitope comprising AEDS (SEQ ID NO: 18), wherein the antibody is
present at a concentration within the range from about 50 mg/mL to
about 100 mg/mL; (b) a buffer; (c) a non-reducing sugar; and (d) a
non-ionic surfactant. In particular examples, the antibody of the
disclosed formulations comprises a light chain comprising an amino
acid sequence set forth as SEQ ID NO: 13 and a heavy chain
comprising an amino acid sequence set forth as SEQ ID NOs: 15.
[0018] In another aspect of the invention, the antibody
formulations are lyophilized. For example, a representative
lyophilized formulation can comprise: (a) a humanized version of
antibody 2A4 (ATCC Accession Number PTA-9662) or antibody 7D8 (ATCC
Accession Number PTA-9468) or antigen binding fragment thereof; (b)
histidine; (c) trehalose; and (d) polysorbate 20. Lyophilized
formulations can have a pH of between about 6 to about 7 when
reconstituted, such as pH 6.5 when reconstituted. Lyophilized
formulations typically comprise about 100 mg to about 1000 mg of
the antibody. Lyophilized formulations typically comprise
polysorbate 20 at a concentration within the range from about
0.005% to about 0.05% by weight. Following reconstitution, the
lyophilized formulations yield an aqueous solution, for example, an
aqueous solution comprising: (a) an antibody comprising a light
chain comprising an amino acid sequence set forth as SEQ ID NO: 13
and a heavy chain comprising an amino acid sequence set forth as
any one of SEQ ID NOs: 14-16, and which is present at a
concentration of about 10 mg/mL; (b) a histidine buffer present at
a concentration of about 25 mM; (c) trehalose present at a
concentration of about 230 mM; (d) polysorbate 20 present at a
concentration of about 0.2 g/L; and (e) a pH of about 6.5. A
representative lyophilized formulation comprises about 100 mg of
the antibody following reconstitution with sterile water.
[0019] Also provided are nucleic acids encoding antibodies used to
prepare the disclosed formulations. For example, such nucleic acids
include nucleic acids comprising nucleotide sequences encoding an
antibody light chain of SEQ ID NO: 13 and nucleic acids comprising
nucleotide sequences encoding an antibody heavy chain of any one of
SEQ ID NOs: 14-16. For example, the nucleotide sequences set forth
as SEQ ID NO: 19 and SEQ ID NO: 20 (which is identical to SEQ ID
NO: 19 and further includes a sequence encoding a signal peptide)
each encode the humanized 2A4 light chain of SEQ ID NO: 13. As
another example, the nucleotide sequences set forth as SEQ ID NO:
22 and SEQ ID NO: 23 (which is identical to SEQ ID NO: 22 and
further includes a sequence encoding a signal peptide) each encode
the humanized 2A4 heavy chain of SEQ ID NO: 15.
[0020] For the production of antibodies, the disclosed nucleic
acids may be included in a vector, either singly or in combination
(e.g., a combination of a nucleic acid encoding a humanized 2A4
light chain and a nucleic acid encoding a humanized 2A4 heavy
chain). For example, a vector can comprise a nucleic acid
comprising a nucleotide sequence encoding any one of SEQ ID NOs:
13-16, 21, and 24; a nucleic acid comprising the nucleotide
sequence of any one of SEQ ID NOs: 19-20 and 22-23, or combinations
thereof. Representative vectors of the invention include (a) a
vector comprising a nucleic acid sequence encoding a humanized 2A4
light chain set forth as SEQ ID NO: 13 or 21 and a humanized 2A
heavy chain set forth as SEQ ID NO: 15 or 24; (b) a vector
comprising a nucleic acid having the nucleotide sequence of SEQ ID
NO: 19 and a nucleic acid having the nucleotide sequence of SEQ ID
NO: 22; and (c) a vector comprising a nucleic acid having the
nucleotide sequence of SEQ ID NO: 20 and a nucleic acid having the
nucleotide sequence of SEQ ID NO: 23.
[0021] Also provided are host cells (e.g., CHO cells) having stably
incorporated into their genomes one or more of the nucleic acids
disclosed herein. For example, a host cell can comprise in its
genome a stably integrated nucleic acid comprising a nucleotide
sequence encoding any one of SEQ ID NOs: 13-16, 21, and 24; a
stably integrated nucleic acid comprising the nucleotide sequence
of any one of SEQ ID NOs: 19-20 and 22-23, or combinations thereof.
Representative host cells of the invention include (a) host cells
comprising a nucleic acid sequence encoding a humanized 2A4 light
chain set forth as SEQ ID NO: 13 or 21 and a humanized 2A heavy
chain set forth as SEQ ID NO: 15 or 24; (b) host cells comprising a
nucleic acid having the nucleotide sequence of SEQ ID NO: 19 and a
nucleic acid having the nucleotide sequence of SEQ ID NO: 22; and
(c) host cells comprising a nucleic acid having the nucleotide
sequence of SEQ ID NO: 20 and a nucleic acid having the nucleotide
sequence of SEQ ID NO: 23.
[0022] The present invention also provides methods of preparing
pharmaceutical formulations. In one aspect of the invention, such a
method comprises (a) culturing mammalian cells having stably
incorporated into their genome nucleic acids encoding the light and
heavy chains of a murine, chimeric or humanized 2A4 antibody or of
a murine, chimeric or humanized 7D8 antibody so that the cells
secrete the antibody into the cell culture media, and purifying the
antibody from the cell culture media; (b) and preparing a
formulation comprising (i) a chimeric or humanized version of
antibody 2A4 (ATCC Accession Number PTA-9662) or of antibody 7D8
(ATCC Accession Number PTA-9468), or fragment thereof, that
specifically competes for binding to antigen with 2A4 or 7D8,
wherein the antibody is present at a concentration within the range
from about 1 mg/mL to about 100 mg/mL; (ii) histidine buffer
present at a concentration within the range from about 20 mM to
about 30 mM; (iii) trehalose present at a concentration within the
range from about 210 mM to about 250 mM; and (iv) polysorbate 20
present at a concentration within the range from about 0.005% to
about 0.05% by weight; wherein the formulation is characterized by
a pH within the range from about 6 to about 7. For example, in one
aspect of the invention, mammalian cells having stably incorporated
into their genomes nucleic acids encoding the light and heavy
chains of a humanized 2A4 antibody are cultured. Mammalian cells
useful for this purpose include (a) host cells having stably
incorporated into their genomes a nucleic acid sequence encoding a
humanized 2A4 light chain set forth as SEQ ID NO: 13 or 21 and a
humanized 2A heavy chain set forth as SEQ ID NO: 15 or 24; (b) host
cells having stably incorporated into their genomes a nucleic acid
having the nucleotide sequence of SEQ ID NO: 19 and a nucleic acid
having the nucleotide sequence of SEQ ID NO: 22; and (c) host cells
having stably incorporated into their genomes a nucleic acid having
the nucleotide sequence of SEQ ID NO: 20 and a nucleic acid having
the nucleotide sequence of SEQ ID NO: 23. In some aspects of the
invention, the disclosed methods of preparing a pharmaceutical
formulation include the additional step of evaluating at least one
property of antibody in the formulation, such as physical
stability, chemical stability, and/or biological activity.
[0023] Still further provided are methods of therapeutically or
prophylactically treating a human patient having or at risk of
having amyloidosis characterized by the presence of amyloid protein
fibrils, the method comprising administering to the patient an
effective dosage of a formulation of the invention. Patients
amenable to treatment have an amyloid disease such as amyloid A
amyloidosis, which is characterized by the presence of amyloid A
protein fibrils, or AL amyloidosis, which is characterized by the
presence of amyloid light chain-type protein fibrils. Patients
having AL amyloidosis may also suffer from an associated dyscrasia
of the B lymphocyte lineage, for example a malignancy such as
multiple myeloma.
[0024] The disclosed therapeutic and prophylactic treatment methods
include combination therapies (i.e., administration of the
disclosed antibody formulations with one or more additional drug
substances) to thereby elicit synergistic results. The two or more
drug substances are administered simultaneously or sequentially in
any order, i.e., a formulation of the invention is administered
prior to administration of a second drug substance, concurrently
with a second drug substance, or subsequent to administration of a
second drug substance. For example, a formulation of the invention
can be administered concurrently or consecutively in combination
with melphalan. As another example, a formulation of the invention
can be administered concurrently or consecutively in combination
with one or more of bortezomib, melphalan, lenalidomide and
carfilzomib.
[0025] In accordance with the disclosed therapeutic and
prophylactic treatment methods, formulations of the invention can
be administered in multiple dosages, for example, at a frequency in
a range of about daily to about annually, such as at a frequency in
a range of about every other week to about every three months, or
such as once a month. In one aspect, an antibody formulation of the
invention is administered intravenously at a dose in a range from
about 10 mg to about 5000 mg drug substance. For example, a
formulation can be administered at a dose in a range from about 30
mg to about 2500 mg humanized 2A4 drug substance at a frequency in
a range of about every other week to about every other month.
Representative dosages used in the disclosed methods include 30 mg,
100 mg, 300 mg, 1000 mg, 2000 mg, and 2500 mg of humanized 2A4 drug
substance.
[0026] In one aspect of the invention, a method of therapeutically
or prophylactically treating a human patient having or at risk for
having light chain (AL) amyloidosis characterized by the presence
of amyloid fibrils, deposits or prefibrillar aggregates, comprises
administering to the patient an effective dosage of a
pharmaceutical formulation comprising: (a) an antibody comprising a
light chain comprising an amino acid sequence set forth as SEQ ID
NO: 13 and a heavy chain comprising an amino acid sequence set
forth as any one of SEQ ID NOs: 14-16, and which is present at a
concentration of about 10 mg/mL; (b) a histidine buffer present at
a concentration of about 25 mM; (c) trehalose present at a
concentration of about 230 mM; (d) polysorbate 20 present at a
concentration of about 0.2 g/L; and (e) a pH of about 6.5. In such
a method, the dosage is typically from about 0.5 mg/kg to about 30
mg/kg of the antibody (e.g., about 0.5 mg/kg to about 8 mg/kg, or
about 8 mg/kg to about 30 mg/kg) administered intravenously or
subcutaneously at a frequency of from about weekly to about
quarterly (e.g., once every 28 days).
[0027] The present invention further provides a pharmaceutical
product comprising: (a) a vial comprising about 100 mg antibody in
powder form; (b) instructions for reconstitution of the antibody;
and (c) instructions for preparing the reconstituted antibody for
infusion, wherein (i) the antibody comprises a light chain
comprising an amino acid sequence set forth as SEQ ID NO: 13 and a
heavy chain comprising an amino acid sequence set forth as any one
of SEQ ID NOs: 14-16; and (ii) the reconstitution instructions
require reconstitution with water for injection to an extractable
volume of 10 mL.
BRIEF DESCRIPTION OF THE DRAWINGS
[0028] FIGS. 1A-1B show various humanized 2A4 antibody light chain
and heavy chain sequences. Bold and underlining, consensus sequence
for N-linked glycosylation.
[0029] FIG. 2 shows murine 2A4 and 7D8 light chain variable region
(VL) and heavy chain variable region (VH) sequences. Double
underlining, leader sequence; underlining, complementarity
determining region (CDR) sequences.
[0030] FIG. 3 shows humanized 2A4 version 3 light chain variable
region (VL) and heavy chain variable region (VH) sequences. Lower
case, back mutations.
[0031] FIGS. 4A-4B show nucleic acid sequences encoding humanized
2A4 version 3 heavy chain (FIG. 4A) and heavy chain (FIG. 4B)
sequences. Single underline, leader sequence; no underline,
variable region; double underline, constant region.
DETAILED DESCRIPTION OF THE INVENTION
[0032] The present invention provides antibody formulations useful
for prophylaxis and treatment of amyloid disease. In one aspect of
the invention, a pharmaceutical formulation comprises (a) a
chimeric or humanized version of antibody 2A4 (ATCC Accession
Number PTA-9662) or of antibody 7D8 (ATCC Accession Number
PTA-9468), or fragment thereof, which specifically competes for
binding to antigen with 2A4 or 7D8, and/or which is directed to an
epitope comprising AEDS (SEQ ID NO: 18), wherein the antibody is
present at a concentration within the range from about 1 mg/mL to
about 100 mg/mL; (b) histidine buffer present at a concentration
within the range from about 20 mM to about 30 mM; (c) trehalose
present at a concentration within the range from about 210 mM to
about 250 mM; and (d) polysorbate 20 present at a concentration
within the range from about 0.005% to about 0.05% by weight;
wherein the formulation is characterized by a pH within the range
from about 6 to about 7.
[0033] In one aspect of the invention described herein, humanized
2A4 is an IgG1, kappa isotype version of murine 2A4. In the course
of specificity characterization of humanized 2A4, the antibody was
found to also react with high affinity and in a
conformation-dependent manner with light chain in light chain
amyloid fibrils, but not with free light chain in circulation.
[0034] The present invention provides methods for intravenous
infusion of humanized 2A4 and/or humanized 7D8 antibodies to target
misfolded amyloid protein in patients with AA amyloidosis and AL
amyloidosis. Some humanized 2A4 antibodies specifically bind to
pathologic amyloid forms of AL and SAA but do not bind to the
parent molecules from which these pathologic forms are derived
(SAA, native immunoglobulin light chain [LC], intact immunoglobulin
[Ig]).
I. Pharmaceutical Formulations and Products
[0035] I.A. Characteristics
[0036] Provided herein are pharmaceutical formulations comprising a
chimeric or humanized version of antibody 2A4 (ATCC Accession
Number PTA-9662) or of antibody 7D8 (ATCC Accession Number
PTA-9468), or fragment thereof, that specifically competes for
binding to antigen (i.e., human AA or AL protein) with 2A4 or 7D8,
respectively, and/or that is directed to the epitope AEDS (SEQ ID
NO: 18). Also provided are pharmaceutical formulations comprising
murine antibody 2A4 or murine antibody 7D8, or fragments thereof.
The antibody is present at a concentration within the range from
about 1 mg/mL to about 100 mg/mL. The formulation is characterized
by a pH within the range from about 6 to about 7 and comprises a
histidine buffer at a concentration within the range from about 20
mM to about 30 mM, trehalose at a concentration within the range
from about 210 mM to about 250 mM; and polysorbate 20 at a
concentration within the range from about 0.005% to about 0.05% by
weight.
[0037] The term "humanized immunoglobulin" or "humanized antibody"
refers to an immunoglobulin or antibody that includes at least one
humanized immunoglobulin or antibody chain (i.e., at least one
humanized light or heavy chain). The term "humanized immunoglobulin
chain" or "humanized antibody chain" (i.e., a "humanized
immunoglobulin light chain" or "humanized immunoglobulin heavy
chain") refers to an immunoglobulin or antibody chain (i.e., a
light or heavy chain, respectively) having a variable region that
includes a variable framework region substantially from a human
immunoglobulin or antibody and complementarity determining regions
(CDRs) (e.g., at least one CDR, preferably two CDRs, more
preferably three CDRs) substantially from a non-human
immunoglobulin or antibody, and further includes constant regions
(e.g., at least one constant region or portion thereof, in the case
of a light chain, and preferably three constant regions in the case
of a heavy chain). The term "humanized variable region" (e.g.,
"humanized light chain variable region" or "humanized heavy chain
variable region") refers to a variable region that includes a
variable framework region substantially from a human immunoglobulin
or antibody and complementarity determining regions (CDRs)
substantially from a non-human immunoglobulin or antibody.
[0038] The phrase "substantially from a human immunoglobulin or
antibody" or "substantially human" means that, when aligned to a
human immunoglobulin or antibody amino sequence for comparison
purposes, the region shares at least 80-90%, preferably 90-95%,
more preferably 95-99% identity (i.e., local sequence identity)
with the human framework or constant region sequence, allowing, for
example, for conservative substitutions, consensus sequence
substitutions, germline substitutions, backmutations, and the like.
The introduction of conservative substitutions, consensus sequence
substitutions, germline substitutions, backmutations, and the like,
is often referred to as "optimization" of a humanized antibody or
chain. The phrase "substantially from a non-human immunoglobulin or
antibody" or "substantially non-human" means having an
immunoglobulin or antibody sequence at least 80-95%, preferably
90-95%, more preferably, 96%, 97%, 98%, or 99% identical to that of
a non-human organism, e.g., a non-human mammal.
[0039] Accordingly, all regions or residues of a humanized
immunoglobulin or antibody, or of a humanized immunoglobulin or
antibody chain, except possibly the CDRs, are substantially
identical to the corresponding regions or residues of one or more
native human immunoglobulin sequences. The term "corresponding
region" or "corresponding residue" refers to a region or residue on
a second amino acid or nucleotide sequence which occupies the same
(i.e., equivalent) position as a region or residue on a first amino
acid or nucleotide sequence, when the first and second sequences
are optimally aligned for comparison purposes.
[0040] In some formulations, the antibody comprises a light chain
variable region comprising an amino acid sequence set forth as any
one of SEQ ID NOs: 1, 2, or 4. In some formulations, the antibody
comprises a heavy chain variable region comprising an amino acid
sequence set forth as SEQ ID NO: 3 or 5. In some formulations, the
antibody comprises a light chain variable region comprising an
amino acid sequence set forth as any one of SEQ ID NOs: 1, 2, or 4
and a heavy chain variable region comprising an amino acid sequence
set forth as SEQ ID NO: 3 or 5. In some formulations, the antibody
comprises a light chain variable region comprising an amino acid
sequence set forth as SEQ ID NO: 1 and a heavy chain variable
region comprising an amino acid sequence set forth as SEQ ID NO: 3.
In some formulations, the antibody comprises a light chain variable
region comprising an amino acid sequence set forth as SEQ ID NO: 4
and a heavy chain variable region comprising an amino acid sequence
set forth as SEQ ID NO: 5. In some formulations, the antibody
comprises a light chain variable region comprising an amino acid
sequence set forth as SEQ ID NO: 2 and a heavy chain variable
region comprising an amino acid sequence set forth as SEQ ID NO:
3.
[0041] In some formulations, the antibody comprises a light chain
variable region comprising three complementarity determining
regions set forth as SEQ ID NOs: 6, 7, and 8, and a heavy chain
variable region comprising three complementarity regions set forth
as SEQ ID NOs: 9, 10, and 11. In other formulations, the antibody
comprises a light chain variable region comprising three
complementarity determining regions set forth as SEQ ID NOs: 12, 7,
and 8, and a heavy chain variable region comprising three
complementarity regions set forth as SEQ ID NOs: 9, 10, and 11.
[0042] In other formulations of the present invention, the antibody
comprises light chain and heavy chain variable regions of a murine,
chimeric, or humanized 2A4 antibody, or of a murine, chimeric, or
humanized 7D8 antibody, as described in U.S. Pat. No. 7,928,203 and
PCT International Publication No. WO 2009/086539, each of which is
incorporated herein by reference in its entirety, and the light
chain and heavy chain variable region sequences described in the
referenced patent and publication are specifically incorporated by
reference herein.
[0043] In some formulations, the antibody comprises a light chain
comprising an amino acid sequence set forth as SEQ ID NO: 13 or 21
and a heavy chain comprising an amino acid sequence set forth as
any one of SEQ ID NOs: 14-16 and 24. The antibody can include, or
not include, the leader sequences of the above-noted light chain
and heavy chain amino acid sequences.
[0044] In other formulations, the antibody is a fragment of a 2A4
or 7D8 antibody, including chimeric and humanized versions thereof,
such as a Fab fragment, a Fab' fragment, a F(ab').sub.2 fragment, a
Fv fragment or a ScFv fragment.
[0045] In some aspects of the invention, the antibody specifically
binds to aggregated amyloid protein without specifically binding to
monomeric amyloid protein (e.g., at least a 10-fold and usually at
least 100-fold lower specific binding affinity for monomeric forms
of the amyloid protein).
[0046] In some formulations, the antibody is present at a
concentration within the range from about 5 mg/mL to about 100
mg/mL. In some formulations, the antibody is present at a
concentration within the range from about 5 mg/mL to about 15
mg/mL. In some formulations, the antibody is present at a
concentration within the range from about 25 mg/mL to about 75
mg/mL. For example, the antibody may be present at a concentration
of about 10mg/mL, or present at a concentration of about 50 mg/mL.
The antibody may be present in a sterile liquid dosage form of
about 50 mg/vial to about 500 mg/vial, or greater. For example, the
antibody may be present in a sterile liquid dosage form of about
100 mg/vial.
[0047] Antibodies used in the disclosed formulations can be coupled
with a therapeutic moiety, such as a cytotoxic agent, a
radiotherapeutic agent, an immunomodulator, a second antibody
(e.g., to form an antibody heteroconjugate), or any other
biologically active agent that facilitates or enhances the activity
of a chimeric or humanized 2A4 or a chimeric or humanized 7D8
antibody. Representative therapeutic moieties include agent known
to be useful for treatment, management, or amelioration of amyloid
disease or symptoms of amyloid disease.
[0048] Antibodies used in the disclosed formulations can also be
coupled with a detectable label, for example, as useful for
diagnosing an amyloid disorder, for monitoring progression of
amyloid disease, and/or for assessing efficacy of treatment.
Antibodies formulated as described are particularly useful for
performing such determinations in subjects having or being
susceptible to AA amyloidosis or AL amyloidosis, or in appropriate
biological samples obtained from such subjects. Representative
detectable labels that may be coupled or linked to a humanized 2A4
antibody or humanized 7D8 antibody include various enzymes, such as
horseradish peroxidase, alkaline phosphatase, beta-galactosidase,
or acetylcholinesterase; prosthetic groups, such
streptavidinlbiotin and avidin/biotin; fluorescent materials, such
as but umbelliferone, fluorescein, fluorescein isothiocynate,
rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or
phycoerythrin; luminescent materials, such as luminol;
bioluminescent materials, such as luciferase, luciferin, and
aequorin; radioactive materials, such as but not limited to iodine
(.sup.131I, .sup.125I, .sup.123I, .sup.121I), carbon (.sup.14C),
sulfur (.sup.5S), tritium (.sup.3H), indium (.sup.115In,
.sup.113In, .sup.112In, .sup.111In,), and technetium (.sup.99Tc),
thallium (.sup.201Ti), gallium (.sup.68Ga, .sup.67Ga), palladium
(.sup.103Pd), molybdenum (.sup.99Mo), xenon (.sup.133Xe), fluorine
(.sup.18F), .sup.153Sm, .sup.177Lu, .sup.159Gd, .sup.149Pm,
.sup.140La, .sup.175Yb, .sup.166Ho, .sup.90Y, .sup.47Sc,
.sup.186Re, .sup.188Re, .sup.142Pr, .sup.105Rh, .sup.97Ru,
.sup.68Ge, .sup.57Co, .sup.65Zn, .sup.85Sr, .sup.32P, .sup.153Gd,
.sup.169Yb, .sup.51Cr, .sup.54Mn, .sup.75Se, .sup.113Sn, and
.sup.117Tin; positron emitting metals using various positron
emission tomographies, nonradioactive paramagnetic metal ions, and
molecules that are radiolabelled or conjugated to specific
radioisotopes.
[0049] Therapeutic moieties and/or detectable substances may be
coupled or conjugated directly to a murine, chimeric or humanized
2A4 antibody or a murine, chimeric or humanized 7D8 antibody, or
indirectly, through an intermediate (e.g., a linker) using
techniques known in the art. See e.g., Arnon et al., "Monoclonal
Antibodies For Immunotargeting Of Drugs In Cancer Therapy", in
Monoclonal Antibodies And Cancer Therapy, Reisfeld et al. (eds.),
pp. 243-56 (Alan R. Liss, Inc. 1985); Hellstrom et al., "Antibodies
For Drug Delivery", in Controlled Drug Delivery (2nd Ed.), Robinson
et al. (eds.), pp. 623-53 (Marcel Dekker, Inc. 1987); Thorpe,
"Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A
Review", in Monoclonal Antibodies 84: Biological And Clinical
Applications, Pinchera et al. (eds.), pp. 475-506 (1985);
"Analysis, Results, And Future Prospective Of The Therapeutic Use
Of Radiolabeled Antibody In Cancer Therapy", in Monoclonal
Antibodies For Cancer Detection And Therapy, Baldwin et al. (eds.),
pp. 303-16 (Academic Press 1985), and Thorpe et al., Immunol. Rev.,
1982, 62:119-58.
[0050] Antibodies used in the disclosed formulations also include
modified forms of murine, chimeric or humanized 2A4 antibodies, or
murine, chimeric or humanized 7D8 antibodies, which have increased
in vivo half-lives relative to the corresponding unmodified
antibodies. Such modified forms may be prepared, for example, by
glycosylation, acetylation, pegylation, phosphorylation, amidation,
derivatization by known protecting/blocking groups, proteolytic
cleavage, linkage to a cellular ligand or other protein, etc. As
one example, representative methods for antibody half-life
extension are described in PCT International Publication No. WO
02/060919.
[0051] The present invention encompasses antibody formulations
having stability at 38.degree. C.-42.degree. C. as assessed by high
performance size exclusion chromatography (HPSEC) for at least
about 30 days, formulations having stability at 20.degree.
C.-24.degree. C. for at least about 1 year, and formulations having
stability at 2.degree. C.-4.degree. C. for at least about 3 years.
More particularly, the disclosed formulations exhibit low to
undetectable levels of antibody aggregation and/or fragmentation,
or a low or undetectable increase of antibody fragmentation and/or
aggregation above an initial level (e.g., less than about 10%
aggregation. A formulation having low to undetectable levels of
fragmentation contains at least about 80%, 85%, 90%, 95%, 98%, or
99%, of the total protein, for example, in a single peak as
determined by high performance size exclusion chromatography
(HPSEC), or in two (2) peaks (one corresponding to each of the
antibody heavy chains and antibody light chains) by reduced
Capillary Gel Electrophoresis (rCGE), representing the non-degraded
antibody, and containing no other single peaks having more than 5%,
more than 4%, more than 3%, more than 2%, more than 1%, or more
than 0.5% of the total protein each. A formulation having low to
undetectable levels of aggregation contains no more than about 15%,
no more than about 10%, no more that about 5%, no more than about
4%, no more than about 3%, no more than about 2%, no more than
about 1%, or no more than about 0.5% aggregation by weight protein
as measured by high performance size exclusion chromatography
(HPSEC). For example, in some formulations, less than about 10% of
the anti-amyloid antibody is present as an aggregate. Stable
formulations of the invention also show little or no loss of
biological activity(ies) of a chimeric or humanized 2A4 or chimeric
or humanized 7D8, for example binding affinity measurable by ELISAs
and/or additional functional assays, such as at least about at
least about 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%,
or 99% of an initial measurable value of a given activity.
[0052] The histidine buffer may be present in some formulations at
a concentration of about 25 mM. In some formulations, the histidine
buffer comprises L-histidine and L-histidine HCl monohydrate. For
example, in some formulations, L-histidine is present at a
concentration within the range from about 16 mM to about 22 mM and
L-histidine HCl monohydrate is present at a concentration within
the range from about 4 mM to about 8 mM.
[0053] In some formulations, trehalose is present at a
concentration from about 210 mM to about 250 mM, for example, about
230 mM. In some formulations, a different non-reducing sugar is
used, such as sucrose, mannitol, or sorbitol.
[0054] In some formulations, polysorbate 20 is present at a
concentration within the range of about from about 0.005% to about
0.05% by weight, for example, 0.005%, 0.01%, 0.015%, 0.02%, 0.025%,
0.03%, 0.035%, 0.04%, 0.045%, or 0.05%. Alternatively, in some
formulations, polysorbate 20 is present at a concentration within
the range of about from about 0.05 g/L, 0.1 g/L, 0.15 g/L, 0.2 g/L,
0.25 g/L, 0.3 g/L, 0.35 g/L, 0.4 g/L, 0.45 g/L, or 0.5 g/L. Some
formulations include polysorbate 20 at a concentration of 0.2
g/L.
[0055] Some formulations are characterized by a pH within the range
of about 6-7, for example, a pH of 6.0, 6.1, 6.2, 6.3, 6.4, 6.5,
6.6, 6.7, 6.8, 6.9, or 7.0. Some formulations have a pH of about
6.5.
[0056] Some formulations are characterized by an osmolality of
about 300 mOsm/kg.
[0057] A bulking agent may also be included some formulations.
[0058] Typically, the formulations are sterile, for example, as
accomplished by sterile filtration using a 0.2 .mu.m or a 0.22
.mu.m filter. The formulations of the invention are also generally
stable upon freezing and thawing.
[0059] Optionally, formulations of the invention may further
comprise other excipients, such as saccharides, polyols, and amino
acids (e.g., arginine, lysine, and methionine). In other aspects,
the present invention also provides formulations substantially free
of surfactant, inorganic salts, additional sugars, and/or other
excipients, i.e., less than about less than 0.0005%, less than
0.0003%, or less than 0.0001% of such compounds.
[0060] An exemplary formulation comprises an antibody comprising a
light chain comprising an amino acid sequence set forth as SEQ ID
NO: 13 and a heavy chain comprising an amino acid sequence set
forth as any one of SEQ ID NOs: 14, 15, or 16, which is present at
a concentration of about 10 mg/mL, a histidine buffer present at a
concentration of about 25 mM, trehalose present at a concentration
of about 230 mM; polysorbate 20 present at a concentration of about
0.2 g/L, and a pH of about 6.5.
[0061] I.B. Preparation of Pharmaceutical Formulations
[0062] The present invention also provides methods of preparing
pharmaceutical formulations. In one aspect of the invention, such a
method comprises (a) culturing mammalian cells having stably
incorporated into their genome nucleic acids encoding the light and
heavy chains of murine antibody 2A4 (ATCC Accession Number
PTA-9662) or of antibody 7D8 (ATCC Accession Number PTA-9468), or
of chimeric or humanized versions thereof, so that the cells
secrete the antibody into the cell culture media, and purifying the
antibody from the cell culture media; (b) and preparing a
formulation comprising (i) the purified antibody present at a
concentration within the range from about 1 mg/mL to about 100
mg/mL; (ii) histidine buffer present at a concentration within the
range from about 20 mM to about 30 mM; (iii) trehalose present at a
concentration within the range from about 210 mM to about 250 mM;
and (iv) polysorbate 20 present at a concentration within the range
from about 0.005% to about 0.05% by weight; wherein the formulation
is characterized by a pH within the range from about 6 to about
7.
[0063] In some aspects of the invention, the disclosed methods of
preparing a pharmaceutical formulation include the additional step
of evaluating at least one property of antibody in the formulation
selected from the group consisting of the physical stability,
chemical stability and biological activity.
[0064] For example, in one aspect of the invention, mammalian cells
are cultured for the production of antibodies, wherein the
mammalian cells have stably incorporated into their genomes nucleic
acids encoding the light and heavy chains of a humanized 2A4
antibody. Mammalian cells useful for this purpose include (a) host
cells having stably incorporated into their genomes a nucleic acid
sequence encoding a humanized 2A4 light chain set forth as SEQ ID
NO: 13 or 21 and a humanized 2A heavy chain set forth as SEQ ID NO:
15 or 24; (b) host cells having stably incorporated into their
genomes a nucleic acid having the nucleotide sequence of SEQ ID NO:
19 and a nucleic acid having the nucleotide sequence of SEQ ID NO:
22; and (c) host cells having stably incorporated into their
genomes a nucleic acid having the nucleotide sequence of SEQ ID NO:
20 and a nucleic acid having the nucleotide sequence of SEQ ID NO:
23.
[0065] For the production of antibodies, the disclosed nucleic
acids are included in a vector. In some examples, the vector
contains the nucleic acid encoding murine 2A4 of 7D8 antibody, or a
chimeric or humanized version thereof, operably linked to a
suitable control sequence capable of effecting the expression of
the DNA in a host cell. Such control sequences include a promoter
to effect transcription (e.g., a constitutive promoter or inducible
promoter as known in the art), an optional operator sequence to
control such transcription, a sequence encoding suitable mRNA
ribosome binding sites, enhancers, polyadenylation signals, and
sequences to control the termination of transcription and
translation. The vector may be a plasmid, a phage particle (e.g., a
viral vector such as adenovirus, adeno-associated-virus,
retrovirus, herpes virus, vaccinia virus, lentivirus, poxvirus and
cytomegalovirus vectors), or simply a genomic insert. Once
transformed into a suitable host, the antibody nucleic acids may
integrate into the genome of the host, or the vector may replicate
and function independently of the host genome.
[0066] The disclosed nucleic acids are included in a vector either
singly or in combination (e.g., a combination of a nucleic acid
encoding an antibody light chain and a nucleic acid encoding an
antibody heavy chain). For example, a vector can comprise a nucleic
acid comprising a nucleotide sequence encoding any one of SEQ ID
NOs: 13-16, 21, or 24; a nucleic acid comprising the nucleotide
sequence of any one of SEQ ID NOs: 19-20 and 22-23, or combinations
thereof. Representative vectors of the invention include (a) a
vector comprising a nucleic acid sequence encoding a humanized 2A4
light chain set forth as SEQ ID NO: 13 and a humanized 2A heavy
chain set forth as SEQ ID NO: 15; (b) a vector comprising a nucleic
acid having the nucleotide sequence of SEQ ID NO: 19 and a nucleic
acid having the nucleotide sequence of SEQ ID NO: 22; and (c) a
vector comprising a nucleic acid having the nucleotide sequence of
SEQ ID NO: 20 and a nucleic acid having the nucleotide sequence of
SEQ ID NO: 23.
[0067] Host cells useful for preparing antibody formulations of the
invention include mammalian cells, including cells of human origin,
such as monkey kidney cells, human embryonic kidney cells, baby
hamster kidney (BHK) cells, Chinese hamster ovary cells (CHO)
cells, mouse sertoli cells, human cervical carcinoma (HeLa) cells,
canine kidney cells, human lung cells, human liver cells, mouse
mammary tumor cells, and NS0 cells. For example, a host cell can
comprise in its genome a stably integrated nucleic acid comprising
a nucleotide sequence encoding any one of SEQ ID NOs: 13-16, 21,
and 24; a stably integrated nucleic acid comprising the nucleotide
sequence of any one of SEQ ID NOs: 19-20 and 22-23, or combinations
thereof. Representative host cells of the invention include (a)
host cells comprising a nucleic acid sequence encoding a humanized
2A4 light chain set forth as SEQ ID NO: 13 or 21 and a humanized 2A
heavy chain set forth as SEQ ID NO: 15 or 24; (b) host cells
comprising a nucleic acid having the nucleotide sequence of SEQ ID
NO: 19 and a nucleic acid having the nucleotide sequence of SEQ ID
NO: 22; and (c) host cells comprising a nucleic acid having the
nucleotide sequence of SEQ ID NO: 20 and a nucleic acid having the
nucleotide sequence of SEQ ID NO: 23.
[0068] In another aspect of the invention, a chimeric or humanized
2A4 antibody or a chimeric or humanized 7D8 antibody is prepared by
chemical synthesis and then used in the disclosed formulations.
[0069] Antibodies used to prepare the disclosed formulations are
typically isolated or purified, i.e., substantially free of
cellular material or other contaminating proteins from the cells in
which they are produced, or substantially free of chemical
precursors or other chemicals when chemically synthesized. For
example, an antibody that is substantially free of cellular
material includes preparations of the antibody having less than
about 30%, 25%, 20%, 15%, 10%, 8%, 5%, 2%, 1%, 0.5%, 0.1%, or less
(by dry weight) of contaminating protein. When an antibody is
recombinantly produced, it is also substantially free of culture
medium such that culture medium represents less than about 30%,
25%, 20%, 15%, 10%, 8%, 5%, 2%, 1%, 0.5%, 0.1%, or less, of the
volume of the protein preparation. When an antibody is produced by
chemical synthesis, it is preferably substantially free of or
separated from chemical precursors or other chemicals involved in
the synthesis of the protein. Accordingly, such antibody
preparations have less than about 30%, 25%, 20%, 15%, 10%, 8%, 5%,
2%, 1%, 0.5%, 0.1%, or less (by dry weight) of chemical precursors
or compounds other than the antibody drug substance. Purification
of recombinantly expressed antibody can utilize any of a number of
methods known in the art, such as, for example, affinity
chromatography, acid treatment, depth filtration, anion exchange
chromatography, cation exchange chromatography, nanofiltration,
ultrafiltration, dialysis and diafiltration.
[0070] The purified antibody drug substance can be adjusted to a
solution comprising any of the formulations described herein,
diluted to the desired concentration and stored until ready for
use. Optionally, the formulation can be stored in concentrated form
until ready for use. Liquid formulations can be stored in frozen
form, under refrigeration or at room temperature, depending upon
their stability profile, which can be determined empirically. In
some instances a further filtration step is applied. Some of the
formulations described herein may be lyophilized and stored in
powder form. Lyophilized formulations can be stored in frozen form,
under refrigeration or at room temperature, depending upon their
stability profile, which can be determined empirically. For
example, the lyophilized formulations can be stored at a
temperature of about 2.degree. C. to 8.degree. C. In such cases,
the formulation would be reconstituted prior to administration to a
patient to yield a liquid formulation having the antibody and
excipients present in the concentrations described herein. In some
cases, the formulation is reconstituted in sterile water. In some
cases, the formulation is reconstituted and added to an infusion
bag for administration to the patient. The reconstituted
formulation can be stored under refrigeration or at room
temperature prior to administration to a patient for a time
consistent with the stability profile. Lyophilization and
reconstitution techniques are known in the art and described in the
Examples.
[0071] Thus, the present invention also encompasses pharmaceutical
products comprising lyophilized antibody drug substance and
instructions for reconstitution and use. For example, a
representative pharmaceutical product can comprise: (a) a vial
comprising about 100 mg antibody in powder form; (b) instructions
for reconstitution of the antibody; and (c) instructions for
preparing the reconstituted antibody for infusion, wherein (i) the
antibody comprises a light chain comprising an amino acid sequence
set forth as SEQ ID NO: 13 and a heavy chain comprising an amino
acid sequence set forth as any one of SEQ ID NOs: 14-16; and (ii)
the reconstitution instructions require reconstitution with water
for injection to an extractable volume of 10 mL.
II. Methods of Diagnosis and Treatment
[0072] Also provided are methods of therapeutically or
prophylactically treating a human patient having or at risk of
having amyloidosis characterized by the presence of amyloid protein
fibrils, the method comprising administering to the patient an
effective dosage of any of the formulations described herein.
[0073] II.A. Subjects Amenable to Diagnosis and Treatment
[0074] Humanized 2A4 drug substance is to be used for the treatment
of systemic amyloidosis, such as amyloidoses involving either
amyloid light chain AL or amyloid A (AA) proteins. Systemic
amyloidoses are a complex group of diseases caused by tissue
deposition of misfolded proteins that result in progressive organ
damage. The most common type, AL amyloidosis or primary
amyloidosis, involves a hematological disorder caused by clonal
plasma cells that produce misfolded immunoglobulin light chains.
Overproduction of misfolded light chain by plasma cells results in
deposits of abnormal AL protein (amyloid), in the tissues and
organs of individuals with AL amyloidosis. Clinical features of AL
amyloidosis include a constellation of symptoms and organ
dysfunction that can include cardiac, renal, and hepatic
dysfunction, GI involvement, neuropathy's and macroglossia. A
different form of systemic amyloidosis, AA amyloidosis or secondary
amyloidosis, occurs "secondarily" as a result of other illness,
such as chronic inflammatory diseases (for example, rheumatoid
arthritis and ankylosing spondylitis) or chronic infections (for
example, tuberculosis or osteomyelitis). In secondary amyloidosis,
the depositing amyloid protein is amyloid A protein, derived from
an acute-phase protein serum amyloid A.
[0075] Peripheral amyloidosis is be amenable to this type of
amyloid-specific immunotherapy through antibody targeting of a
neo-epitope that has been identified in AA amyloid, as well as in
AL amyloid. Studies in animal models of both AA and AL have
demonstrated that significant positive therapeutic effects may be
possible at reasonable doses of antibody.
[0076] Subjects or patients amenable to treatment using the
disclosed antibody formulations include individuals at risk of
disease but not showing symptoms, as well as patients presently
showing symptoms of amyloid disease. Therefore, the present methods
can be administered prophylactically to the general population
without the need for any assessment of the risk of the subject
patient. For example, the present methods are especially useful for
individuals who do have a known genetic risk autoimmune disorders.
Such individuals include those having relatives who have
experienced this disease and those whose risk is determined by
analysis of genetic or biochemical markers. As another example,
patients suffering from AA amyloidosis can be asymptomatic for a
prolonged period of time, such that clinical diagnosis of AA
amyloidosis is often delayed or missed until the amyloid deposits
are extensive. For those patients who are symptomatic, it is
estimated that only 53% of the cases are diagnosed. See e.g.,
L.E.K. Consulting, Independent Market Research (2003). Prophylactic
administration disclosed antibody formulations may reduce the
incidence of AA amyloidosis.
[0077] The present methods are especially useful for individuals
who do have a known risk of, are suspected to have, or have been
diagnosed with AA amyloidosis or AL amyloidosis. Such individuals
include but are not limited to those having chronic inflammatory
diseases, inherited inflammatory diseases, and chronic microbial
infections, such as rheumatoid arthritis, juvenile chronic
arthritis, ankylosing spondylitis, psoriasis, psoriatic
arthropathy, Reiter's syndrome, Adult Still's disease, Behcet's
syndrome, Crohn's disease, Familial Mediterranean Fever, leprosy,
tuberculosis, bronchiectasis, decubitus ulcers, chronic
pyelonephritis, osteomyelitis, Whipple's disease, myeloma,
macroglobulinemia, immunocyte dyscrasia, monoclonal gammopathy,
occult dyscrasia. Chronic inflammatory and infectious conditions
are prerequisite to the development of AA amyloidosis and AL
amyloidosis manifested by local nodular amyloidosis can be
associated with chronic inflammatory diseases. Individuals who do
have known risk of AA amyloidosis also include but are not limited
to those having malignant neoplasms as Hodgkin's lymphoma, renal
carcinoma, carcinomas of gut, lung and urogenital tract, basal cell
carcinoma, and hairy cell leukemia. Additionally, individuals with
known risk of AA amyloidosis also include but are not limited to
those having lymphoproliferative disorders such as Castleman's
Disease. Some of such patients have AA amyloidosis characterized by
the presence of amyloid A protein fibrils. Some of such patients
have AL amyloidosis characterized by the presence of amyloid light
chain-type protein fibrils. Some patients have systemic organ
dysfunction attributed to AL amyloidosis, including dysfunction of
the heart, kidney, liver, peripheral nervous system,
gastrointestinal system, autonomic nervous system, lung, and/or
soft tissue or lymphatic system.
[0078] Patients amenable to treatment also include those patients
who have received, are currently receiving, or will later receive
an alternate therapy, for treatment of amyloid disease or an
associated condition, such as, inflammatory diseases, chronic
microbial infections, malignant neoplasms, inherited inflammatory
diseases, and lymphoproliferative disorders. For example, patients
may also receive or have received one or more of the therapeutic
agents identified herein with respect to combination therapies. As
a particular example, patients suffering from AL may also receive
or have received bortezomib, melphalan, lenalidomide and/or
carfilzomib. For those patients who have previously received
alternate therapies for the treatment of amyloid disease, such
therapies may or may not have been successful by the relevant
clinical measures.
[0079] II.B. Treatment Regimes
[0080] As used herein, the terms "treat" and "treatment" refer to
the alleviation or amelioration of one or more symptoms or effects
associated with the disease, prevention, inhibition or delay of the
onset of one or more symptoms or effects of the disease, lessening
of the severity or frequency of one or more symptoms or effects of
the disease, and/or increasing or trending toward desired outcomes
as described herein.
[0081] Desired outcomes of the treatments disclosed herein vary
according to the amyloid disease and patient profile and are
readily determinable to those skilled in the art. Generally,
desired outcomes include measurable indices such as reduction or
clearance of pathologic amyloid fibrils, decreased or inhibited
amyloid aggregation and/or deposition of amyloid fibrils, and
increased immune response to pathologic and/or aggregated amyloid
fibrils. Desired outcomes also include amelioration of amyloid
disease-specific symptoms. For example, desired outcomes for the
treatment of AL amyloidosis include a decrease in the incidence or
severity of known symptoms, including organ dysfunction, peripheral
and autonomic neuropathy, carpal tunnel syndrome, macroglossia,
restrictive cardiomyopathy, arthropathy of large joints, immune
dyscrasias, myelomas, as well as occult dyscrasias. As another
example, desired outcomes for the treatment of AA include a
decrease in associated inflammation, arthritis, psoriasis,
microbial infection, malignancy, or symptoms of other preexisting
or coexisting disease to which the AA amyloidosis is secondary.
[0082] Desired outcomes of the disclosed therapies are generally
quantifiable measures as compared to a control or baseline
measurement. As used herein, relative terms such as "improve,"
"increase," or "reduce" indicate values relative to a control, such
as a measurement in the same individual prior to initiation of
treatment described herein, or a measurement in a control
individual or group. A control individual is an individual
afflicted with the same amyloid disease as the individual being
treated, who is about the same age as the individual being treated
(to ensure that the stages of the disease in the treated individual
and the control individual are comparable), but who has not
received treatment using the disclosed antibody formulations. In
this case, efficacy of the disclosed antibody formulations is
assessed by a shift or trend away from measurable indices in the
untreated control. Alternatively, a control individual is a healthy
individual, who is about the same age as the individual being
treated. In this case, efficacy of the disclosed antibody
formulations is assessed by a shift or trend toward from measurable
indices in the healthy control. Changes or improvements in response
to therapy are generally statistically significant and described by
a p-value less than or equal to 0.1, less than 0.05, less than
0.01, less than 0.005, or less than 0.001 may be regarded as
significant.
[0083] In both asymptomatic and symptomatic patients, treatment
according to the disclosed methods can begin at any time before or
after the diagnosis of the underlying AA or AL amyloid diseases.
Treatment typically entails multiple dosages over a period of time.
Treatment can be monitored by assaying antibody, or employing
radiolabeled SAP Scintigraphy over time. If the response falls, a
booster dosage may be indicated. The response of patients with AL
amyloidosis to treatment can be monitored by assessing cardiac
markers, such as NT-proBNP and/or troponin, serum creatine, and/or
alkaline phosphatase; by performing serum free light chain (SFLC)
assays, quantitative immunoglobulin assays, biopsies, serum protein
electrophoresis (SPEP), urine protein electrophoresis (UPEP),
serum, urine immunofixation electrophoresis (IFE), and/or organ
imaging techniques. An exemplary complete response (CR) can be
determined from response criteria including negative IFE of serum
and urine, normal .kappa./.lamda. ration and/or <5% plasma cells
in bone marrow. An exemplary very good partial response (VGPR) can
be determined from a dFLC of .ltoreq.40 mg/L. An exemplary partial
response (PR) can be determined from a dFLC decrease of
.gtoreq.50%. In the kidney, a response to treatment can be
determined, for example, from a .gtoreq.50% reduction (e.g.,
>0.5g/24 hours) in 24 hour urine protein excretion in the
absence of either a reduction in eGFR of .gtoreq.25% or an increase
in serum creatine of .gtoreq.0.5 mg/dL. In the liver, a response to
treatment can be determined, for example, from a .gtoreq.50%
reduction in initially elevated alkaline phosphatase or a .gtoreq.2
cm reduction in liver size on CT scan or MRI. In the heart, a
response to treatment can be determined, for example, from a
.gtoreq.30% and 300 ng/L reduction in NT-proBNP in patients with
baseline of NT-proBNP of >650 ng/L.
[0084] The antibody formulation can be administered intravenously
in dosage ranges from about 10 mg to about 5000 mg for the patient
in question, such as, for example, about 10 mg, about 30 mg, about
100 mg, about 300 mg, about 1000 mg, about 2000 mg, or about 2500
mg. The antibody formulation can also be administered intravenously
in dosage ranges from about 0.1 mg/kg to about 50 mg/kg, or from
about 0.5 mg/kg to about 30 mg/kg, of the host body weight. For
example, dosages can be about 0.5 mg/kg body weight, about 1.0
mg/kg, about 1.5 mg/kg, about 2.0 mg/kg, about 4.0 mg/kg, about 5.0
mg/kg, about 8.0 mg/kg, about 10 mg/kg, about 15 mg/kg, about 16
mg/kg, about 20 mg/kg, about 25 mg/kg, or about 30 mg/kg body
weight. Dose escalation for an individual patient can occur at the
discretion of the prescriber in the absence of any clinically
significant occurrence that the prescriber might reasonably believe
would present an undue safety risk for the patient, such as, for
example, Grade.gtoreq.3 non-hematologic toxicity, Grade.gtoreq.3
nausea, vomiting or diarrhea uncontrolled by maximum
antiemetic/anti-diarrhea therapy, Grade 4 neutropenia lasting >7
days in the absence of growth factor support, Grade 3 or 4
neutropenia of any duration accompanied with
fever.gtoreq.38.5.degree. C. and/or systemic infection, or other
Grade.gtoreq.4 hematologic toxicity.
[0085] Antibody is usually administered on multiple occasions. An
exemplary treatment regime entails administration once per every
two weeks, once a month, or once every 3 to 6 months. For example,
patients can receive the antibody formulation once every four weeks
as a cycle, for example every twenty-eight days. The dosing
frequency can be adjusted depending on the pharmacokinetic profile
of the antibody formulation in the patient. For example, the
half-life of the antibody may warrant a two week frequency of
dosing. In some methods, two or more monoclonal antibodies with
different binding specificities are administered simultaneously, in
which case the dosage of each antibody administered falls within
the ranges indicated. Intervals between single dosages can be
weekly, monthly or yearly. Intervals can also be irregular as
indicated by measuring blood levels of antibody to amyloid protein
(e.g., AA) in the patient. In some methods, dosage is adjusted to
achieve a plasma antibody concentration of about 1-1000 .mu.g/ml or
about 25-300 .mu.g/ml. Alternatively, antibody can be administered
as a sustained release formulation, in which case less frequent
administration is required.
[0086] Dosage and frequency vary depending on the half-life of the
antibody in the patient. In general, human antibodies show the
longest half life, followed by humanized antibodies, chimeric
antibodies, and nonhuman antibodies. The dosage and frequency of
administration can vary depending on whether the treatment is
prophylactic or therapeutic. In prophylactic applications, a
relatively low dosage is administered at relatively infrequent
intervals over a long period of time. Some patients continue to
receive treatment for the rest of their lives. In therapeutic
applications, a relatively high dosage at relatively short
intervals is sometimes required until progression of the disease is
reduced or terminated, until a partial or complete response is
achieved, and/or until the patient shows lessening or amelioration
of symptoms of disease. Thereafter, the patent can be administered
a prophylactic regime.
[0087] The formulations disclosed herein may be provided in a
dosage form that is suitable for parenteral (e.g., intravenous,
intramuscular, subcutaneous) administration. As appropriate for
particular applications, the formulation may be alternately
provided in a dosage suitable for rectal, transdermal, nasal,
vaginal, inhalant, ocular or other administration. The
pharmaceutical formulations are typically prepared according to
conventional pharmaceutical practice. See e.g., Remington: The
Science and Practice of Pharmacy, (19th ed.) ed. A. R. Gennaro,
1995, Mack Publishing Company, Easton, Pa. and Encyclopedia of
Pharmaceutical Technology, eds. J. Swarbrick and J. C. Boylan,
1988-1999, Marcel Dekker, N.Y.
[0088] In one aspect of the invention, a method of therapeutically
or prophylactically treating a human patient having or at risk for
having light chain (AL) amyloidosis characterized by the presence
of amyloid fibrils, deposits or prefibrillar aggregates, comprises
administering to the patient an effective dosage of a
pharmaceutical formulation comprising: (a) an antibody comprising a
light chain comprising an amino acid sequence set forth as SEQ ID
NO: 13 and a heavy chain comprising an amino acid sequence set
forth as any one of SEQ ID NOs: 14-16, and which is present at a
concentration of about 10 mg/mL; (b) a histidine buffer present at
a concentration of about 25 mM; (c) trehalose present at a
concentration of about 230 mM; (d) polysorbate 20 present at a
concentration of about 0.2 g/L; and (e) a pH of about 6.5. In such
a method, the dosage is typically from about 0.5 mg/kg to about 30
mg/kg of the antibody (e.g., about 0.5 mg/kg to about 8 mg/kg, or
about 8 mg/kg to about 30 mg/kg) administered intravenously or
subcutaneously at a frequency of from about weekly to about
quarterly (e.g., once every 28 days).
[0089] II.C. Combinational Drug Therapy Treatment Regimes
[0090] The present invention also encompasses combination therapies
for treatment or prophylaxis of amyloid disease, particularly AA
amyloidosis and AL amyloidosis. Such combination therapies are
performed by administering an antibody formulation of the invention
in conjunction with one or more second therapeutic agents, such as
another therapy to treat or effect prophylaxis of AA amyloidosis or
AL amyloidosis, as the case may be. Combination therapy according
to the invention may also be performed in conjunction with a second
therapy is used to treat or effect prophylaxis of a disease or
condition associated with amyloid disease, such as an inflammatory
disease, a chronic microbial infection, a neoplasm (including
malignant neoplasms), an inherited inflammatory disease, and/or a
lymphoproliferative disorder. Numerous treatments are available in
commercial use, in clinical evaluation, and in pre-clinical
development, any of which could be selected for use in combination
with the disclosed antibody formulations. Such treatments can be
one or more compounds or treatments selected from, but not limited
to several major categories, namely, (i) non-steroidal
anti-inflammatory drugs (NSAIDs; e.g., detoprofen, diclofenac,
diflunisal, etodolac, fenoprofen, flurbiprofen, ibuprofen,
indomethacin, ketoprofen, meclofenameate, mefenamic acid,
meloxicam, nabumeone, naproxen sodium, oxaprozin, piroxicam,
sulindac, tolmetin, celecoxib, rofecoxib, aspirin, choline
salicylate, salsalte, and sodium and magnesium salicylate); (ii)
steroids (e.g., cortisone, dexamethasone, hydrocortisone,
methylprednisolone, prednisolone, prednisone, triamcinolone); (iii)
DMARDs, i.e., disease modifying antirheumatic drugs (e.g.,
cyclosporine, azathioprine, methotrexate, leflunomide,
cyclophosphamide, hydroxychloroquine, sulfasalazine,
D-penicillamine, minocycline, and gold); (iv) recombinant proteins
(e.g., ENBREL.RTM. (etanercept, a soluble TNF receptor) and
REMICADE.RTM. (infliximab) a chimeric monoclonal anti-TNF
antibody); (v) stem cell transplantation; and/or (vi) chemotherapy.
Patients with AL amyloidosis may also receive treatment regimes
that include drugs or combinations of drugs often used to treat
hematological malignancies, such as melphalan, prednisone,
dexamethasone, lenalidomide (REVLIMID.RTM.) and proteosome
inhibitors such as bortezomib (VELCADE.RTM.), and carfilzomib
(KYPROLIS.TM.), at dosages in the range of the standard of
care.
[0091] The duration of the combination therapy depends on the type
of amyloid disease being treated, any underlying disease associated
with the amyloid disease, the age and condition of the patient, the
stage and type of the patient's disease, how the patient responds
to the treatment, etc. A medical doctor can observe the therapy's
effects closely and make any adjustments as needed. Additionally, a
person having a greater risk of developing AA amyloidosis (e.g., a
person who is genetically predisposed or previously had an
inflammatory disorder or other underlying diseases) or AL
amyloidosis may receive prophylactic combination treatments to
inhibit or delay the development of AA AL aggregates such as
fibrils, or as maintenance therapy post-treatment.
[0092] When performing a combination therapy, the two or more drug
substances are administered simultaneously or sequentially in any
order, i.e., a formulation of the invention is administered prior
to administering a second drug substance, concurrently with a
second drug substance, or subsequent to administration of a second
drug substance. For example, a combination therapy may be performed
by administering a first therapy prior to (e.g., 1 minute, 5
minutes, 15 minutes, 30 minutes, 45 minutes, 1 hour, 2 hours, 4
hours, 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 96 hours, 1
week, 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 8 weeks, or 12
weeks before), concomitantly with, or subsequent to (e.g., 1
minute, 5 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hour, 2
hours, 4 hours, 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 96
hours, 1 week, 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 8
weeks, or 12 weeks after) administering a second agent/therapy.
[0093] The dosage, frequency and mode of administration of each
component of the combination can be controlled independently. For
example, one therapeutic agent/therapy may be administered orally
three times per day, while the second therapeutic agent/therapy may
be administered intramuscularly once per day. Combination therapy
may be given in on-and-off cycles that include rest periods. The
compounds may also be admixed or otherwise formulated together such
that one administration delivers both compounds. In this case, each
therapeutic agent is generally present in an amount of 1-95% by
weight of the total weight of the composition. Alternatively, an
antibody formulation of the invention and a second therapeutic
agent can be formulated separately and in individual dosage
amounts. Drug combinations for treatment can be provided as
components of a pharmaceutical pack.
[0094] Preferably, the disclosed combination therapies elicit a
synergistic therapeutic effect, i.e., an effect greater than the
sum of their individual effects or therapeutic outcomes. Measurable
therapeutic outcomes are described herein. For example, a
synergistic therapeutic effect may be an effect of at least about
two-fold greater than sum of the therapeutic effects elicited by
the single agents of a given combination, or at least about
five-fold greater, or at least about ten-fold greater, or at least
about twenty-fold greater, or at least about fifty-fold greater, or
at least about one hundred-fold greater. A synergistic therapeutic
effect may also be observed as an increase in therapeutic effect of
at least 10% compared to the sum of the therapeutic effects
elicited by the single agents of a given combination, or at least
20%, or at least 30%, or at least 40%, or at least 50%, or at least
60%, or at least 70%, or at least 80%, or at least 90%, or at least
100%, or more. A synergistic effect is also an effect that permits
reduced dosing of therapeutic agents when they are used in
combination.
EXAMPLES
[0095] The following examples have been included to illustrate
modes of the invention. Certain aspects of the following examples
are described in terms of techniques and procedures found or
contemplated by the present co-inventors to work well in the
practice of the invention. In light of the present disclosure and
the general level of skill in the art, those of skill appreciate
that the following examples are intended to be exemplary only and
that numerous changes, modifications, and alterations may be
employed without departing from the scope of the invention.
Example 1
Selection of Humanized 2A4 for the Treatment of AL Amyloidosis
[0096] An IgG1, kappa isotype antibody was prepared, which is a
humanized version of murine antibody 2A4. The light chain and heavy
chain sequences of representative humanized 2A4 antibodies are set
forth in FIGS. 1A-1B and 3. Nucleic acids encoding the particular
humanized 2A4 antibody version 3, which amino acid sequences are
shown in FIG. 3, are depicted in FIGS. 4A-4B.
[0097] The parent monoclonal 2A4 antibody is directed against a
neo-carboxy terminal epitope of human serum Amyloid A (sAA),
resulting from cleavage of the native sAA molecule at amino acid
residue 76. The murine antibody does not cross-react with IgGs or
free light chain (LC) and it has shown broad isotype recognition of
patient derived AL amyloid samples examined to date. 2A4 recognizes
multiple forms of AL light chain amyloid including soluble multimer
and insoluble deposits. In addition, the antibody has been shown to
promote regression of amyloidoma in a mouse xenograft model. The
light chain and heavy chain sequences of murine 2A4 antibody are
set forth in FIG. 2.
Example 2
Dose Determination for Humanized 2A4 Antibody
[0098] Nonclinical studies in the TRIAD mouse model and the
cynomolgus monkey have utilized doses of 4 and 40 mg/kg in the
mouse and 10, 50, and 100 mg/kg in the monkey. Conversion to the
Human Equivalent Dose (HED) on a mg/kg basis (most appropriate
conversion for monoclonal antibodies due to their restriction to
the vascular space) gives HEDs of 0.32 and 3.2 for the mouse and
3.2, 16, and 32 for the monkey. Based on currently available data,
the NOAEL in both species is expected to be the highest dose
administered. Using a mouse HED (most sensitive species due to
dosing limitations) of 3.2 and a 10.times. safety factor, the MRSD
for first in man dosing would be approximately 0.32 mg/kg. Based
upon animal studies, administration to humans is begun with a dose
of 0.5 mg/kg.
Example 3
Preparation of the Expression Vector
[0099] For generation of the final h2A4 IgG1 HC vector the variable
region of the heavy chain was isolated by PCR using the plasmid
CET1019AS-hygro-h2A4VH3-Sce 4.23.07 as template. Primers used for
the amplification introduced at the 5' end of the fragments an MfeI
restriction site and at the 3' end a BlpI restriction site for
subcloning. The variable region was cloned into the MfeI and BamHI
digested eukaryotic expression vector pBI-61, which contains the
genomic constant regions of human IgG1 of G1m(3) allotype. The
resulting recombinant expression vector pBI-61/2A4 IgG1-REM is
9,015 base pairs in size and carries the selectable marker
dihydrofolate reductase (DHFR) from hamster under the control of
the DHFR promoter and polyadenylation signal. This vector also
contains the beta-lactamase gene for selection in E. coli as well
as origins of replication for E. coli (ColE1 ori), SV40 (SV40 ori)
and filamentous phage f1 (f1 ori). Expression of the HC is driven
by the immediate early promoter/enhancer region from human
cytomegalovirus (CMV) combined with a transcription enhancing
element (TE) derived from the hamster genome. For transcript
termination and stabilization the polyadenylation signal from
hamster growth hormone is used and for enhancement of transcription
a non-coding sequence derived from the hamster genome (TE).
[0100] Using the plasmid CET1019AS-hu2A4VL3-hck-euro-Sce 4.19.07 as
template the variable region of the h2A4 LC was isolated by PCR
introducing at the 5' end of the fragments an SgrAI restriction
site and at the 3' end a KpnI restriction site for subcloning into
the final eukaryotic expression vector pBI-60 digested with the
same restriction enzymes. This vector contains the genomic constant
region of a human kappa chain. The resulting recombinant expression
vector pBI-60/2A4 LC is 7,144 base pairs in size and contains the
selectable marker neomycin phosphotransferase mutant, which confers
resistance to geneticin, under the control of the SV40 promoter.
For transcript termination the polyadenylation signal from Herpes
simplex thymidine kinase is used. This vector also contains the
beta-lactamase gene for selection in E. coli as well as origins of
replication for E. coli (ColE1 ori) and filamentous phage f1 (f1
ori). Expression of the LC is driven by the immediate early
promoter/enhancer region from human cytomegalovirus (CMV) combined
with a transcription enhancing element (TE) derived from the
hamster genome. For transcript termination and stabilization the
polyadenylation signal from hamster growth hormone is used and for
enhancement of transcription a non-coding sequence derived from the
hamster genome (TE).
Example 4
Production of Humanized 2A4 Antibody (Pool-Derived Material)
[0101] Humanized 2A4 was produced in Chinese Hamster Ovary (CHO)
cells, grown in chemically defined media without any bovine-derived
components. Antibody was pooled from stable transfected cells from
which the production cell line was ultimately derived. The
pool-derived material was purified by protein A-affinity
chromatography. This material was used for human tissue
cross-reactivity studies and for a single dose pharmacokinetic (PK)
study in cynomolgus monkeys. The formulation of the humanized 2A4
antibody is 10 mg/mL antibody, 25 mM L-Histidine/L-Histidine HCl
monohydrate, 230 mM Trehalose dehydrate, 0.02% (w/v) Polysorbate
(TWEEN.RTM.) 20, pH=6.5.
Example 5
Production of Humanized 2A4 Antibody (Clone-Derived Material)
[0102] A single CHO cell clone was isolated from cell pools as
described in Example 3, and was used to establish the Master Cell
Bank (MCB) without any bovine materials. Humanized 2A4 for
nonclinical studies was manufactured at 80 L scale using the same
cell cultivation and purification processes (except scale-up
modifications) as the GMP clinical version of humanized 2A4 (2,000
L scale). Material from the 2,000 L scale production may also be
used in nonclinical studies.
Example 6
Process of Manufacturing Humanized 2A4 Antibody
[0103] Vial Thaw & Inoculum Expansion. Cells from the MCB are
thawed and transferred into an appropriate cell culture flask. The
cells are incubated at approximately 37.degree. C. The thawed
culture is propagated for one to four days (first passage after
cell thaw). For sub-cultivation, an aliquot of a grown cell culture
(and a defined volume of pre-warmed, 0.22 .mu.m or less filtered
inoculum medium) is used to reach a seed density of approximately
0.1-0.5.times.10.sup.6 cells/mL in standard cell culture vessels of
approximately 0.02 L to 1 L working volume. As an example, the
first passages can be done in 0.125 L or 0.25 L or 0.5 L vessels,
followed by passages in 1 L vessels. A stock culture can be
initiated at this cultivation stage. For preparation of inoculum
cultures for individual production fermenters, aliquots of the
stock cultures are expanded to generate cultures with up to 25 L
volume. Typically, the cell culture is scaled up from 1 L cultures
to 2 or more 1 L or 2 L cultures, then to 2 or more 2 L or 3 L
cultures and finally to 2 or more cultures with up to 25 L culture
volume per vessel. Grown cell suspensions from several vessels can
be pooled and used to inoculate the 80 L bioreactor. Shake flasks,
T-flasks, spinner flasks and bags can be used as standard cell
culture vessels for the above cultivation steps.
[0104] Seed Cultures in Bioreactors. Before inoculation with cells,
0.22 .mu.m or less filtered growth medium is added to the
bioreactors. The content of the filled bioreactors is warmed to
approximately 37.degree. C. and maintained at this temperature
throughout incubation of the cells. Cells from the inoculum
cultures are transferred into the pre-warmed medium. The initial
cell density is targeted within the range of 0.1-0.5.times.10.sup.6
cells/mL. The cells are grown in an 80 L bioreactor and
subsequently in a 400 L bioreactor. Cells are subcultivated
approximately every two to four days. At this stage, cells may be
transferred to another vessel of the same or larger volume.
Typically, the cell culture is scaled up from 1.times.80 L
bioreactor culture to 1.times.400 L culture. To initiate the
production phase, the cells are transferred from the grown 400 L
cell suspension to the production bioreactor of approximately 2,000
L working volume.
[0105] Production culture in 2,000 L Bioreactor. Before inoculation
with cells, 0.22 .mu.m or less filtered production medium is added
to the production bioreactor. The content of the filled production
bioreactor is warmed to approximately 37.degree. C. and maintained
at this temperature throughout incubation of the cells. The initial
cell density in the production phase is targeted within the range
of 0.1-0.5.times.10.sup.6 cells/mL. The production bioreactor is
run in a fed batch mode. To support the production of antibody and
to prolong culture duration, a nutrient feed medium is added during
the production stage. The point at which to start feeding is
determined either by culture time or by cell density. As needed, a
glucose solution and/or glutamine solution can be added during the
production stage to avoid depletion of these substances during the
production period. The run time of the 2,000 L production
bioreactor is typically 8 to 14 days. Pre-harvest samples are
tested for sterility, mycoplasma, and adventitious virus in
vitro.
[0106] Harvest and Clarification. After 8 to 14 days of cultivation
in the production phase, the cell culture fluid is separated from
the cells. After pre-harvest sampling and prior to harvest, the pH
and the temperature of the culture can be adjusted to facilitate
removal of cells, debris and particles during harvest. To remove
the cells, the culture is passed through a centrifugation plus
dead-end filtration unit. The cells are centrifuged and/or retained
by the membranes. The harvested culture fluid is passed through
filters of 0.22 .mu.m pore size or less and collected in an
appropriate container. Residual culture fluid can be removed from
the harvest system by flushing with Phosphate Buffered Saline (PBS)
to recover residual product from the harvest system. The resulting
recovered product amount is collected together with the harvested
culture fluid to form the harvest pool, also called harvested
cell-free culture fluid (HCCF). The pH and temperature of the HCCF
can be adjusted to facilitate the subsequent downstream processing
steps.
[0107] Purification. The antibody is purified from the HCCF by a
series of steps involving affinity chromatography, acid treatment,
depth filtration, anion exchange chromatography, cation exchange
chromatography, nanofiltration and ultra-/diafiltration, several of
which may be performed in several cycles. To remove contaminants
the affinity chromatography process step specifically binds the
antibody product. The HCCF is applied to the chromatography column
packed with the MabSelect matrix. The matrix binds antibody at
neutral pH, while contaminants appear in the flow through and are
removed. The column is eluted in a step elution with a 100 mM
acetic acid/sodium acetate solution at pH 3.5. To inactivate
potential viral contaminants, the antibody solution is incubated at
room temperature for a minimum of 60 minutes at pH 3.5.+-.0.1.
After incubation the acid treated pool is adjusted to pH 7.2 using
a 2 M Trometamol solution and subjected to depth filtration for
clarification. For anion exchange chromatography, the depth
filtered product pool is adjusted to a conductivity .ltoreq.7 mS/cm
with Water for Injection (WFI). The adjusted pool is applied to a
chromatography column packed with Q Sepharose FF resin. The
antibody passes through the anion exchange matrix unbound. The flow
through is monitored and the antibody containing fraction is
collected based on absorbance measurement. For cation exchange
chromatography, the product pool is adjusted to a pH of 5.5.+-.0.1
by addition of acetic acid up to a conductivity of <7.5 mS/cm
with WFI. The adjusted product pool is applied onto a
chromatography column packed with SP Sepharose FF cation exchange
resin. This chromatography step is performed in a bind-elute mode.
The antibody binds to the cation exchange matrix. The column is
eluted in a step elution with a 100 mM acetic acid/sodium acetate
and 138.5 mM sodium chloride solution at pH 5.5. Potential viral
contaminants are removed by passing the antibody solution through a
0.1 .mu.m prefilter and a Planova 20N nanofilter at a maximum
pressure of 1 bar differential pressure of the Planova 20N
nanofilter. During ultrafiltration/diafiltration (UF/DF), the
product is concentrated to the target concentration, and the buffer
is exchanged with the formulation buffer. Concentration and
diafiltration is performed using ultrafiltration membranes having a
cut-off of approximately 30 kD. The material is processed by
concentrating the product to 30-100 mg/mL. The 30 kD pool is then
diafiltered with a solution of 25 mM L-Histidine, pH 6.5 and is
flushed to a concentration of about 60-70 mg/mL. The 30 kD pool
intermediate may be stored at -40.degree. C. until formulation is
performed. For formulation, the 30 kD product pool is adjusted to a
solution containing 17.5 mM L-Histidine/7.5 mM L-Histidine
Hydrochloride, 230 mM Trehalose, and 0.02% (w/v) Polysorbate20,
pH=6.5. The antibody is finally diluted with formulation buffer to
the desired target concentration of 10 mg/mL. The resulting drug
substance is filtered through a 0.22 .mu.m filter to remove any
potential adventitious microbial contaminants and particulate
material. The drug substance can be stored frozen at -40.degree. C.
until filling.
Example 7
Characterization of Drug Substance Containing Humanized 2A4
Antibody
[0108] Humanized 2A4 used for formulation is composed of two
heterodimers. Each of the heterodimers is composed of a heavy
polypeptide chain of .about.50 kDa (449 amino acids) and a kappa
light polypeptide chain of .about.24 kDa (219 amino acids). The
antibody protein has a humanized amino acid sequence with a total
molecular mass of approximately 147 kDa. The four polypeptide
chains of the antibody molecule are linked together by disulfide
bonds. Each heavy polypeptide chain contains one consensus sequence
for N-linked glycosylation, which is occupied (positions 299 to
301, highlighted in bold and underlining in FIG. 1A). There are two
binding sites for the serum amyloid A epitope per antibody
molecule.
[0109] A competitive binding ELISA has been established to measure
binding of humanized 2A4 to its antigen (CGGHEDT (SEQ ID NO: 17)
when conjugated to Ovalbumin) compared to the reference
standard.
Example 8
Humanized 2A4 Drug Substance Components and Composition
[0110] The humanized 2A4 drug substance (100 mg/vial) for clinical
use is a sterile liquid dosage form consisting of a 10 mL fill in a
25 mL vial (20R). The nonclinical humanized 2A4 drug substance (200
mg/vial) is 20 mL fill in a 25 mL vial (20R). The nonclinical and
clinical formulations of humanized 2A4 are provided in Table 1. The
final formulation of the humanized 2A4 drug substance has a density
of 1.034 g/mL at 20.degree. C. and a pH of 6.5.
TABLE-US-00001 TABLE 1 Composition of Nonclinical and Clinical
Humanized 2A4 Drug Substance Nominal amount (mg/vial) Concen-
Nonclinical Clinical tration Vial Size = Vial Size = Component
Function (g/L) 25 mL (20 R) 25 mL (20 R) Humanized Active 10 200
100 2A4 drug Substance substance L-Histidine Buffer 2.72 54.4 27.2
component L-Histidine Buffer 1.57 31.4 15.7 HCl mono- component
hydrate Trehalose Tonicity 87.02 1,740.4 870.2 dihydrate agent
Polysorbate Surfactant 0.20 4.0 2.0 (TWEEN .RTM.) 20 Water for
Solvent -- Add WFI Add WFI Injection to a total to a total (WFI)
volume of volume of 20 mL 10 mL
Example 9
Batch Formula for Drug Product (100mg/ml Vial)
[0111] A formula was designed for a 2,600 vial batch of drug
product as provided in Table 2.
TABLE-US-00002 TABLE 2 Batch Formula for 2,600 Vials Ingredient
Grade Quantity per Batch Humanized 2A4 antibody -- 260.0 g
L-Histidine USP, Ph. Eur. 70.72 g L-Histidine HCl monohydrate Ph.
Eur. 40.82 g Trehalose dehydrate USP/NF, Ph. Eur. 2,262.52 g
Polysorbate 20 USP/NF, Ph. Eur. 5.20 g
Example 10
Lyophilization
[0112] A Hof Com 26041 freeze dryer was used to lyophilize the
formulated humanized 2A4 drug substance over a period of
approximately 86 hours with the pressure regulated by an MKS
control system (MKS Instruments) with N.sub.2 injection according
to the program set forth in Table 3. The endpoint was detected by
Pirani signal. During the drying mode, the vials stand directly on
the shelves without lyo plates. The nitrogen backfill is at
approximately 600 mbar with pharma grade, sterile N.sub.2. The
vials were then closed and sorted at 5.degree. C. within the freeze
dryer. The final drug product is stored at 2-8.degree. C.,
protected from light. The process should yield a white to yellowish
lyo cake.
[0113] Table 3 summarizes the program for the lyophilization of
humanized 2A4 drug substance.
TABLE-US-00003 TABLE 3 Lyophilization Steps Step Time Shelf
temperature Vacuum MKS Step No. [hh:mm] [.degree. C.] [mbar]
Loading 01 00:01 5 off Freezing 02 00:15 5 off 03 00:05 2 off 04
02:00 2 off 05 01:05 -50 off 06 02:30 -50 off Primary 07 00:05 -50
0.10 Drying 08 00:40 -10 0.10 09 55:00 -10 0.10 Secondary 10 04:30
30 0.10 Drying 11 20:00 30 0.10 Total Time 86:11
Example 11
Reconstitution of Lyophilized Drug Product
[0114] Prior to application, the lyophlisate has to be
reconstituted with sterilized water for injection. The
reconstitution of h2A4 vials has been performed according to the
following procedure under laminar air-flow. The complete
flip-off-cap of the respective product vial was removed. The
rubber-stopper was also removed. The solvent was added by pipetting
the necessary volume (2.times.5mL WFI using a piston pipette). When
performing this action, it was ensured that the solvent was added
slowly to the lyophilized product. The vials were carefully swirled
(not shaken), until the lyophilized product was completely
dissolved. The solution was made homogenous by carefully rotating
the vial end-over-end. The dissolved material was aliquoted
according to table 1 and stored at -70.degree. C. until
analysis
Example 12
Clinical Assessment of Humanized 2A4 Drug Substance
[0115] A clinical trial is designed to determine a maximum
tolerated dose (MTD) and/or the Phase 2 recommended dose (P2RD) of
humanized 2A4 drug substance in subjects with AL amyloidosis.
Dosing will begin at 0.5 mg/kg and escalate to a high of 30 mg/kg
or 2500 mg total (whichever is lower). Initially, humanized 2A4
drug substance will be given intravenously as a single agent every
28 days until progression of organ function or unacceptable
treatment related toxicity or withdraw of consent. If the half-life
(t.sub.112) of humanized 2A4 drug substance from the initial doses
suggests that a different dosing schedule would be more appropriate
(e.g., every two weeks or an alternate, less frequent schedule than
once every 28 days), dosing in subsequent cohorts may be modified
using an alternative dosing schedule.
Sequence CWU 1
1
241131PRTMus musculusMISC_FEATURE(1)..(19)LEADER SEQUENCE 1Met Lys
Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala -15 -10 -5
Ser Ser Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val -1
1 5 10 Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser
Leu 15 20 25 Val His Ser Thr Gly Asn Thr Tyr Leu His Trp Tyr Leu
Gln Lys Pro 30 35 40 45 Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val
Ser Asn Arg Phe Ser 50 55 60 Gly Val Pro Asp Arg Phe Ser Gly Ser
Gly Ser Gly Thr Tyr Phe Thr 65 70 75 Leu Lys Ile Ser Arg Val Glu
Ala Glu Asp Leu Gly Val Tyr Phe Cys 80 85 90 Ser Gln Ser Thr His
Val Pro Phe Thr Phe Gly Gly Gly Thr Lys Leu 95 100 105 Glu Ile Lys
110 2131PRTMus musculusMISC_FEATURE(1)..(19)LEADER SEQUENCE 2Met
Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala -15 -10
-5 Ser Ser Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val
-1 1 5 10 Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Leu
Ser Leu 15 20 25 Val His Ser Thr Gly Asn Thr Tyr Leu His Trp Tyr
Leu Gln Lys Pro 30 35 40 45 Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys
Val Ser Asn Arg Phe Ser 50 55 60 Gly Val Pro Asp Arg Phe Ser Gly
Ser Gly Ser Gly Thr Tyr Phe Thr 65 70 75 Leu Lys Ile Ser Arg Val
Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys 80 85 90 Ser Gln Ser Thr
His Val Pro Phe Thr Phe Gly Gly Gly Thr Lys Leu 95 100 105 Glu Ile
Lys 110 3138PRTMus musculusMISC_FEATURE(1)..(19)LEADER SEQUENCE
3Met Val Leu Gly Leu Lys Trp Val Phe Phe Val Val Phe Tyr Gln Gly
-15 -10 -5 Val His Cys Glu Val Gln Leu Val Glu Ser Gly Gly Arg Leu
Val Gln -1 1 5 10 Pro Lys Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser
Gly Phe Thr Phe 15 20 25 Asn Thr Tyr Ala Met Tyr Trp Ile Arg Gln
Ala Pro Gly Lys Gly Leu 30 35 40 45 Glu Trp Val Ala Arg Ile Arg Ser
Lys Ser Asn Asn Tyr Ala Ile Tyr 50 55 60 Tyr Ala Asp Ser Val Lys
Asp Arg Phe Thr Ile Phe Arg Asp Asp Ser 65 70 75 Gln Ser Met Leu
Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr 80 85 90 Ala Met
Tyr Tyr Cys Val Arg Pro Tyr Ser Asp Ser Phe Ala Tyr Trp 95 100 105
Gly Gln Gly Thr Leu Val Thr Val Ser Ala 110 115 4112PRTArtificial
SequenceHumanized antibody sequence containing murine and human
residues (humanized 2A4 light chain variable region version 3) 4Asp
Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 1 5 10
15 Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser
20 25 30 Thr Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly
Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe
Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
Asp Phe Thr Leu Lys Ile 65 70 75 80 Ser Arg Val Glu Ala Glu Asp Val
Gly Val Tyr Tyr Cys Ser Gln Ser 85 90 95 Thr His Val Pro Phe Thr
Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 110
5119PRTArtificial SequenceHumanized antibody sequence containing
murine and human residues (humanized 2A4 heavy chain variable
region version 3) 5Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val
Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly
Phe Thr Phe Asn Thr Tyr 20 25 30 Ala Met Tyr Trp Ile Arg Gln Ala
Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ala Arg Ile Arg Ser Lys
Ser Asn Asn Tyr Ala Ile Tyr Tyr Ala Asp 50 55 60 Ser Val Lys Asp
Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Ser 65 70 75 80 Leu Tyr
Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 85 90 95
Tyr Cys Ala Arg Pro Tyr Ser Asp Ser Phe Ala Tyr Trp Gly Gln Gly 100
105 110 Thr Leu Val Thr Val Ser Ser 115 616PRTMus
musculusMISC_FEATURE(1)..(16)2A4 VL CDR1 6Arg Ser Ser Gln Ser Leu
Val His Ser Thr Gly Asn Thr Tyr Leu His 1 5 10 15 77PRTMus
musculusMISC_FEATURE(1)..(7)2A4 VL CDR2 7Lys Val Ser Asn Arg Phe
Ser 1 5 89PRTMus musculusMISC_FEATURE(1)..(9)2A4 VL CDR3 8Ser Gln
Ser Thr His Val Pro Phe Thr 1 5 910PRTMus
musculusMISC_FEATURE(1)..(10)2A4 VH CDR1 9Gly Phe Thr Phe Asn Thr
Tyr Ala Met Tyr 1 5 10 1019PRTMus musculusMISC_FEATURE(1)..(19)2A4
VH CDR2 10Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Ile Tyr Tyr Ala
Asp Ser 1 5 10 15 Val Lys Asp 118PRTMus
musculusMISC_FEATURE(1)..(8)2A4 VH CDR3 11Pro Tyr Ser Asp Ser Phe
Ala Tyr 1 5 1216PRTMus musculusMISC_FEATURE(1)..(16)7D8 VL CDR1
12Arg Ser Ser Leu Ser Leu Val His Ser Thr Gly Asn Thr Tyr Leu His 1
5 10 15 13219PRTArtificial SequenceHumanized antibody sequence
containing murine and human residues (humanized 2A4 kappa light
chain) 13Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr
Pro Gly 1 5 10 15 Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser
Leu Val His Ser 20 25 30 Thr Gly Asn Thr Tyr Leu His Trp Tyr Leu
Gln Lys Pro Gly Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Lys Val
Ser Asn Arg Phe Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser
Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65 70 75 80 Ser Arg Val Glu
Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 85 90 95 Thr His
Val Pro Phe Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 110
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 115
120 125 Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
Phe 130 135 140 Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn
Ala Leu Gln 145 150 155 160 Ser Gly Asn Ser Gln Glu Ser Val Thr Glu
Gln Asp Ser Lys Asp Ser 165 170 175 Thr Tyr Ser Leu Ser Ser Thr Leu
Thr Leu Ser Lys Ala Asp Tyr Glu 180 185 190 Lys His Lys Val Tyr Ala
Cys Glu Val Thr His Gln Gly Leu Ser Ser 195 200 205 Pro Val Thr Lys
Ser Phe Asn Arg Gly Glu Cys 210 215 14449PRTArtificial
SequenceHumanized antibody sequence containing murine and human
residues (humanized 2A4 IgG1 heavy chain variant 1 (G1m1 allotype))
14Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1
5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr
Tyr 20 25 30 Ala Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu
Glu Trp Val 35 40 45 Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala
Ile Tyr Tyr Ala Asp 50 55 60 Ser Val Lys Asp Arg Phe Thr Ile Ser
Arg Asp Asp Ser Lys Asn Ser 65 70 75 80 Leu Tyr Leu Gln Met Asn Ser
Leu Lys Thr Glu Asp Thr Ala Val Tyr 85 90 95 Tyr Cys Ala Arg Pro
Tyr Ser Asp Ser Phe Ala Tyr Trp Gly Gln Gly 100 105 110 Thr Leu Val
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 115 120 125 Pro
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 130 135
140 Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160 Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro
Ala Val Leu 165 170 175 Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val
Val Thr Val Pro Ser 180 185 190 Ser Ser Leu Gly Thr Gln Thr Tyr Ile
Cys Asn Val Asn His Lys Pro 195 200 205 Ser Asn Thr Lys Val Asp Lys
Arg Val Glu Pro Lys Ser Cys Asp Lys 210 215 220 Thr His Thr Cys Pro
Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 225 230 235 240 Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 260
265 270 Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
Asn 275 280 285 Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
Tyr Arg Val 290 295 300 Val Ser Val Leu Thr Val Leu His Gln Asp Trp
Leu Asn Gly Lys Glu 305 310 315 320 Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile Glu Lys 325 330 335 Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 340 345 350 Leu Pro Pro Ser
Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 355 360 365 Cys Leu
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 385
390 395 400 Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
Asp Lys 405 410 415 Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met His Glu 420 425 430 Ala Leu His Asn His Tyr Thr Gln Lys Ser
Leu Ser Leu Ser Pro Gly 435 440 445 Lys 15449PRTArtificial
SequenceHumanized antibody sequence containing murine and human
residues (humanized 2A4 IgG1 heavy chain variant 2 (G1m3 allotype))
15Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1
5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr
Tyr 20 25 30 Ala Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu
Glu Trp Val 35 40 45 Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala
Ile Tyr Tyr Ala Asp 50 55 60 Ser Val Lys Asp Arg Phe Thr Ile Ser
Arg Asp Asp Ser Lys Asn Ser 65 70 75 80 Leu Tyr Leu Gln Met Asn Ser
Leu Lys Thr Glu Asp Thr Ala Val Tyr 85 90 95 Tyr Cys Ala Arg Pro
Tyr Ser Asp Ser Phe Ala Tyr Trp Gly Gln Gly 100 105 110 Thr Leu Val
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 115 120 125 Pro
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 130 135
140 Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160 Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro
Ala Val Leu 165 170 175 Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val
Val Thr Val Pro Ser 180 185 190 Ser Ser Leu Gly Thr Gln Thr Tyr Ile
Cys Asn Val Asn His Lys Pro 195 200 205 Ser Asn Thr Lys Val Asp Lys
Arg Val Glu Pro Lys Ser Cys Asp Lys 210 215 220 Thr His Thr Cys Pro
Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 225 230 235 240 Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 260
265 270 Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
Asn 275 280 285 Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
Tyr Arg Val 290 295 300 Val Ser Val Leu Thr Val Leu His Gln Asp Trp
Leu Asn Gly Lys Glu 305 310 315 320 Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile Glu Lys 325 330 335 Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 340 345 350 Leu Pro Pro Ser
Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 355 360 365 Cys Leu
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 385
390 395 400 Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
Asp Lys 405 410 415 Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met His Glu 420 425 430 Ala Leu His Asn His Tyr Thr Gln Lys Ser
Leu Ser Leu Ser Pro Gly 435 440 445 Lys 16445PRTArtificial
SequenceHumanized antibody sequence containing murine and human
residues (humanized 2A4 IgG2 heavy chain) 16Glu Val Gln Leu Val Glu
Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu
Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 20 25 30 Ala Met
Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45
Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Ile Tyr Tyr Ala Asp 50
55 60 Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn
Ser 65 70 75 80 Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr
Ala Val Tyr 85 90 95 Tyr Cys Ala Arg Pro Tyr Ser Asp Ser Phe Ala
Tyr Trp Gly Gln Gly 100 105 110 Thr Leu Val Thr Val Ser Ser Ala Ser
Thr Lys Gly Pro Ser Val Phe 115 120 125 Pro Leu Ala Pro Cys Ser Arg
Ser Thr Ser Glu Ser Thr Ala Ala Leu 130 135 140 Gly Cys Leu Val Lys
Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 145 150 155 160 Asn Ser
Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 180
185 190 Ser Asn Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His Lys
Pro 195 200 205 Ser Asn Thr Lys Val Asp Lys Thr Val Glu Arg Lys Cys
Cys Val Glu 210 215 220 Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly
Pro Ser Val Phe Leu 225 230 235 240 Phe Pro Pro Lys Pro Lys Asp Thr
Leu Met Ile Ser Arg Thr Pro Glu 245 250 255 Val Thr Cys Val Val Val
Asp Val Ser His Glu Asp Pro Glu Val Gln 260 265 270 Phe Asn
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 275 280 285
Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu 290
295 300 Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
Lys 305 310 315 320 Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys
Thr Ile Ser Lys 325 330 335 Thr Lys Gly Gln Pro Arg Glu Pro Gln Val
Tyr Thr Leu Pro Pro Ser 340 345 350 Arg Glu Glu Met Thr Lys Asn Gln
Val Ser Leu Thr Cys Leu Val Lys 355 360 365 Gly Phe Tyr Pro Ser Asp
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 370 375 380 Pro Glu Asn Asn
Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly 385 390 395 400 Ser
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 405 410
415 Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
420 425 430 His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 435
440 445 177PRTHomo sapiens 17Cys Gly Gly His Glu Asp Thr 1 5
184PRTHomo sapiens 18Ala Glu Asp Ser 1 19660DNAArtificial
SequenceHumanized antibody sequence containing murine and human
residues (Hu2A4 VH3VL3 hcg1,k cDNA sequence - light chain without
signal sequence) 19gacgtggtga tgacccagtc ccctctgtcc ctgcctgtga
cccctggcga gcctgcctcc 60atctcctgcc ggtcctccca gtccctggtg cactccaccg
gcaacaccta tctgcactgg 120tatctgcaga agcctggcca gtctcctcag
ctgctgatct acaaggtgtc caaccggttc 180tccggcgtgc ctgaccggtt
ctctggctcc ggctccggca ccgacttcac cctgaagatc 240tcccgggtgg
aggccgagga cgtgggcgtg tactactgct cccagtccac ccacgtgcct
300ttcaccttcg gcggaggcac caaggtggag atcaagcgaa ctgtggctgc
accatctgtc 360ttcatcttcc cgccatctga tgagcagttg aaatctggaa
ctgcctctgt tgtgtgcctg 420ctgaataact tctatcccag agaggccaaa
gtacagtgga aggtggataa cgccctccaa 480tcgggtaact cccaggagag
tgtcacagag caggacagca aggacagcac ctacagcctc 540agcagcaccc
tgacgctgag caaagcagac tacgagaaac acaaagtcta cgcctgcgaa
600gtcacccatc agggcctgag ctcgcccgtc acaaagagct tcaacagggg
agagtgttag 66020726DNAArtificial SequenceHumanized antibody
sequence containing murine and human residues (Hu2A4 VH3VL3 hcg1,k
cDNA sequence - light chain) 20atg gac atg cgg gtg ccc gca cag ctg
ctg ggc ctg ctg atg ctg tgg 48Met Asp Met Arg Val Pro Ala Gln Leu
Leu Gly Leu Leu Met Leu Trp 1 5 10 15 gtg tcc ggc tcc tcc ggc gac
gtg gtg atg acc cag tcc cct ctg tcc 96Val Ser Gly Ser Ser Gly Asp
Val Val Met Thr Gln Ser Pro Leu Ser 20 25 30 ctg cct gtg acc cct
ggc gag cct gcc tcc atc tcc tgc cgg tcc tcc 144Leu Pro Val Thr Pro
Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser 35 40 45 cag tcc ctg
gtg cac tcc acc ggc aac acc tat ctg cac tgg tat ctg 192Gln Ser Leu
Val His Ser Thr Gly Asn Thr Tyr Leu His Trp Tyr Leu 50 55 60 cag
aag cct ggc cag tct cct cag ctg ctg atc tac aag gtg tcc aac 240Gln
Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn 65 70
75 80 cgg ttc tcc ggc gtg cct gac cgg ttc tct ggc tcc ggc tcc ggc
acc 288Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly
Thr 85 90 95 gac ttc acc ctg aag atc tcc cgg gtg gag gcc gag gac
gtg ggc gtg 336Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp
Val Gly Val 100 105 110 tac tac tgc tcc cag tcc acc cac gtg cct ttc
acc ttc ggc gga ggc 384Tyr Tyr Cys Ser Gln Ser Thr His Val Pro Phe
Thr Phe Gly Gly Gly 115 120 125 acc aag gtg gag atc aag cga act gtg
gct gca cca tct gtc ttc atc 432Thr Lys Val Glu Ile Lys Arg Thr Val
Ala Ala Pro Ser Val Phe Ile 130 135 140 ttc ccg cca tct gat gag cag
ttg aaa tct gga act gcc tct gtt gtg 480Phe Pro Pro Ser Asp Glu Gln
Leu Lys Ser Gly Thr Ala Ser Val Val 145 150 155 160 tgc ctg ctg aat
aac ttc tat ccc aga gag gcc aaa gta cag tgg aag 528Cys Leu Leu Asn
Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 165 170 175 gtg gat
aac gcc ctc caa tcg ggt aac tcc cag gag agt gtc aca gag 576Val Asp
Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 180 185 190
cag gac agc aag gac agc acc tac agc ctc agc agc acc ctg acg ctg
624Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu
195 200 205 agc aaa gca gac tac gag aaa cac aaa gtc tac gcc tgc gaa
gtc acc 672Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu
Val Thr 210 215 220 cat cag ggc ctg agc tcg ccc gtc aca aag agc ttc
aac agg gga gag 720His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe
Asn Arg Gly Glu 225 230 235 240 tgt tag 726Cys 21241PRTArtificial
SequenceSynthetic Construct 21Met Asp Met Arg Val Pro Ala Gln Leu
Leu Gly Leu Leu Met Leu Trp 1 5 10 15 Val Ser Gly Ser Ser Gly Asp
Val Val Met Thr Gln Ser Pro Leu Ser 20 25 30 Leu Pro Val Thr Pro
Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser 35 40 45 Gln Ser Leu
Val His Ser Thr Gly Asn Thr Tyr Leu His Trp Tyr Leu 50 55 60 Gln
Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn 65 70
75 80 Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly
Thr 85 90 95 Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp
Val Gly Val 100 105 110 Tyr Tyr Cys Ser Gln Ser Thr His Val Pro Phe
Thr Phe Gly Gly Gly 115 120 125 Thr Lys Val Glu Ile Lys Arg Thr Val
Ala Ala Pro Ser Val Phe Ile 130 135 140 Phe Pro Pro Ser Asp Glu Gln
Leu Lys Ser Gly Thr Ala Ser Val Val 145 150 155 160 Cys Leu Leu Asn
Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 165 170 175 Val Asp
Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 180 185 190
Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 195
200 205 Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val
Thr 210 215 220 His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn
Arg Gly Glu 225 230 235 240 Cys 221350DNAArtificial
SequenceHumanized antibody sequence containing murine and human
residues (Hu2A4 VH3VL3 hcg1,k cDNA sequence - heavy chain without
signal sequence) 22gaggtgcagc tggtcgagtc cggcggaggc ctggtgcagc
ctggcggctc cctgagactg 60tcctgcgccg cctccggctt caccttcaac acctacgcca
tgtactggat caggcaggct 120cctggcaagg gactggagtg ggtggcccgg
atcaggtcca agtccaacaa ctacgctatc 180tactacgccg actccgtgaa
ggaccggttc accatctccc gggacgactc caagaactcc 240ctgtatctgc
agatgaactc cctgaaaacc gaggacaccg ccgtgtacta ctgcgctcgg
300ccttactccg actccttcgc ctactggggc cagggcaccc tggtgaccgt
gtccagcgcc 360tccaccaagg gcccatcggt cttccccctg gcaccctcct
ccaagagcac ctctgggggc 420acagcggccc tgggctgcct ggtcaaggac
tacttccccg aaccggtgac ggtgtcgtgg 480aactcaggcg ccctgaccag
cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 540ctctactccc
tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac
600atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagagagt
tgagcccaaa 660tcttgtgaca aaactcacac atgcccaccg tgcccagcac
ctgaactcct ggggggaccg 720tcagtcttcc tcttcccccc aaaacccaag
gacaccctca tgatctcccg gacccctgag 780gtcacatgcg tggtggtgga
cgtgagccac gaagaccctg aggtcaagtt caactggtac 840gtggacggcg
tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc
900acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa
tggcaaggag 960tacaagtgca aggtctccaa caaagccctc ccagccccca
tcgagaaaac catctccaaa 1020gccaaagggc agccccgaga accacaggtg
tacacgctgc ccccatcccg ggaggagatg 1080accaagaacc aggtcagcct
gacctgcctg gtcaaaggct tctatcccag cgacatcgcc 1140gtggagtggg
agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg
1200gactccgacg gctccttctt cctctatagc aagctcaccg tggacaagag
caggtggcag 1260caggggaacg tcttctcatg ctccgtgatg catgaggctc
tgcacaacca ctacacgcag 1320aagagcctct ccctgtcccc gggtaaatga
1350231407DNAArtificial SequenceHumanized antibody sequence
containing murine and human residues (Hu2A4 VH3VL3 hcg1,k cDNA
sequence - heavy chain) 23atg gag ttc ggc ctg tcc tgg ctg ttc ctg
gtg gcc atc ctg aag ggc 48Met Glu Phe Gly Leu Ser Trp Leu Phe Leu
Val Ala Ile Leu Lys Gly 1 5 10 15 gtg cag tgc gag gtg cag ctg gtc
gag tcc ggc gga ggc ctg gtg cag 96Val Gln Cys Glu Val Gln Leu Val
Glu Ser Gly Gly Gly Leu Val Gln 20 25 30 cct ggc ggc tcc ctg aga
ctg tcc tgc gcc gcc tcc ggc ttc acc ttc 144Pro Gly Gly Ser Leu Arg
Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 35 40 45 aac acc tac gcc
atg tac tgg atc agg cag gct cct ggc aag gga ctg 192Asn Thr Tyr Ala
Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu 50 55 60 gag tgg
gtg gcc cgg atc agg tcc aag tcc aac aac tac gct atc tac 240Glu Trp
Val Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Ile Tyr 65 70 75 80
tac gcc gac tcc gtg aag gac cgg ttc acc atc tcc cgg gac gac tcc
288Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser
85 90 95 aag aac tcc ctg tat ctg cag atg aac tcc ctg aaa acc gag
gac acc 336Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu
Asp Thr 100 105 110 gcc gtg tac tac tgc gct cgg cct tac tcc gac tcc
ttc gcc tac tgg 384Ala Val Tyr Tyr Cys Ala Arg Pro Tyr Ser Asp Ser
Phe Ala Tyr Trp 115 120 125 ggc cag ggc acc ctg gtg acc gtg tcc agc
gcc tcc acc aag ggc cca 432Gly Gln Gly Thr Leu Val Thr Val Ser Ser
Ala Ser Thr Lys Gly Pro 130 135 140 tcg gtc ttc ccc ctg gca ccc tcc
tcc aag agc acc tct ggg ggc aca 480Ser Val Phe Pro Leu Ala Pro Ser
Ser Lys Ser Thr Ser Gly Gly Thr 145 150 155 160 gcg gcc ctg ggc tgc
ctg gtc aag gac tac ttc ccc gaa ccg gtg acg 528Ala Ala Leu Gly Cys
Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 165 170 175 gtg tcg tgg
aac tca ggc gcc ctg acc agc ggc gtg cac acc ttc ccg 576Val Ser Trp
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 180 185 190 gct
gtc cta cag tcc tca gga ctc tac tcc ctc agc agc gtg gtg acc 624Ala
Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 195 200
205 gtg ccc tcc agc agc ttg ggc acc cag acc tac atc tgc aac gtg aat
672Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn
210 215 220 cac aag ccc agc aac acc aag gtg gac aag aga gtt gag ccc
aaa tct 720His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro
Lys Ser 225 230 235 240 tgt gac aaa act cac aca tgc cca ccg tgc cca
gca cct gaa ctc ctg 768Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro
Ala Pro Glu Leu Leu 245 250 255 ggg gga ccg tca gtc ttc ctc ttc ccc
cca aaa ccc aag gac acc ctc 816Gly Gly Pro Ser Val Phe Leu Phe Pro
Pro Lys Pro Lys Asp Thr Leu 260 265 270 atg atc tcc cgg acc cct gag
gtc aca tgc gtg gtg gtg gac gtg agc 864Met Ile Ser Arg Thr Pro Glu
Val Thr Cys Val Val Val Asp Val Ser 275 280 285 cac gaa gac cct gag
gtc aag ttc aac tgg tac gtg gac ggc gtg gag 912His Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 290 295 300 gtg cat aat
gcc aag aca aag ccg cgg gag gag cag tac aac agc acg 960Val His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 305 310 315 320
tac cgt gtg gtc agc gtc ctc acc gtc ctg cac cag gac tgg ctg aat
1008Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
325 330 335 ggc aag gag tac aag tgc aag gtc tcc aac aaa gcc ctc cca
gcc ccc 1056Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
Ala Pro 340 345 350 atc gag aaa acc atc tcc aaa gcc aaa ggg cag ccc
cga gaa cca cag 1104Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
Arg Glu Pro Gln 355 360 365 gtg tac acg ctg ccc cca tcc cgg gag gag
atg acc aag aac cag gtc 1152Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
Met Thr Lys Asn Gln Val 370 375 380 agc ctg acc tgc ctg gtc aaa ggc
ttc tat ccc agc gac atc gcc gtg 1200Ser Leu Thr Cys Leu Val Lys Gly
Phe Tyr Pro Ser Asp Ile Ala Val 385 390 395 400 gag tgg gag agc aat
ggg cag ccg gag aac aac tac aag acc acg cct 1248Glu Trp Glu Ser Asn
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 405 410 415 ccc gtg ctg
gac tcc gac ggc tcc ttc ttc ctc tat agc aag ctc acc 1296Pro Val Leu
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 420 425 430 gtg
gac aag agc agg tgg cag cag ggg aac gtc ttc tca tgc tcc gtg 1344Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 435 440
445 atg cat gag gct ctg cac aac cac tac acg cag aag agc ctc tcc ctg
1392Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
450 455 460 tcc ccg ggt aaa tga 1407Ser Pro Gly Lys 465
24468PRTArtificial SequenceSynthetic Construct 24Met Glu Phe Gly
Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 1 5 10 15 Val Gln
Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 20 25 30
Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 35
40 45 Asn Thr Tyr Ala Met Tyr Trp Ile Arg Gln Ala Pro Gly Lys Gly
Leu 50 55 60 Glu Trp Val Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr
Ala Ile Tyr 65 70 75 80 Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile
Ser Arg Asp Asp Ser 85 90 95 Lys Asn Ser Leu Tyr Leu Gln Met Asn
Ser Leu Lys Thr Glu Asp Thr 100 105 110 Ala Val Tyr Tyr Cys Ala Arg
Pro Tyr Ser Asp Ser Phe Ala Tyr Trp 115 120 125 Gly Gln Gly Thr Leu
Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 130 135 140 Ser Val Phe
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 145 150 155 160
Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 165
170 175 Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
Pro 180 185 190 Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser
Val Val Thr 195 200 205 Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr
Ile Cys
Asn Val Asn 210 215 220 His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg
Val Glu Pro Lys Ser 225 230 235 240 Cys Asp Lys Thr His Thr Cys Pro
Pro Cys Pro Ala Pro Glu Leu Leu 245 250 255 Gly Gly Pro Ser Val Phe
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 260 265 270 Met Ile Ser Arg
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 275 280 285 His Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 290 295 300
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 305
310 315 320 Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
Leu Asn 325 330 335 Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
Leu Pro Ala Pro 340 345 350 Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
Gln Pro Arg Glu Pro Gln 355 360 365 Val Tyr Thr Leu Pro Pro Ser Arg
Glu Glu Met Thr Lys Asn Gln Val 370 375 380 Ser Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 385 390 395 400 Glu Trp Glu
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 405 410 415 Pro
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 420 425
430 Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
435 440 445 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu 450 455 460 Ser Pro Gly Lys 465
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