U.S. patent application number 14/610193 was filed with the patent office on 2015-10-22 for two-part disinfectant system and related methods.
The applicant listed for this patent is Solutions BioMed, LLC. Invention is credited to Brian G. Larson, Daryl J. Tichy.
Application Number | 20150297770 14/610193 |
Document ID | / |
Family ID | 42170696 |
Filed Date | 2015-10-22 |
United States Patent
Application |
20150297770 |
Kind Code |
A1 |
Larson; Brian G. ; et
al. |
October 22, 2015 |
TWO-PART DISINFECTANT SYSTEM AND RELATED METHODS
Abstract
The present disclosure is drawn to a disinfectant system which
can be used to disinfect surfaces. The system includes a first
chamber containing a first solution and a second chamber containing
a second solution. The first solution can include an alcohol, an
organic carboxylic acid, and from 0.01 ppm, to 1,000 ppm by weight
of a transition metal or alloy thereof based on the first solution
weight content. The second solution can include hydrogen peroxide.
The system further includes a dispenser through which the system is
configured to mix and dispense the first solution and the second
solution immediately before being dispensed. A peracid composition
is formed upon mixing of the first and second solutions.
Inventors: |
Larson; Brian G.; (Alpine,
UT) ; Tichy; Daryl J.; (Orem, UT) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Solutions BioMed, LLC |
Orem |
UT |
US |
|
|
Family ID: |
42170696 |
Appl. No.: |
14/610193 |
Filed: |
January 30, 2015 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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14269443 |
May 5, 2014 |
8987331 |
|
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14610193 |
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12617521 |
Nov 12, 2009 |
8716339 |
|
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14269443 |
|
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61113946 |
Nov 12, 2008 |
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Current U.S.
Class: |
222/145.1 |
Current CPC
Class: |
A01N 59/16 20130101;
A61L 2/18 20130101; A01N 31/02 20130101; A01N 59/00 20130101; A01N
31/02 20130101; A01N 59/16 20130101; A01N 37/36 20130101; A01N
31/02 20130101; A01N 59/16 20130101; A61L 2/186 20130101; A01N
59/00 20130101; A01N 31/02 20130101; A01N 37/16 20130101; A01N
37/36 20130101; A01N 37/16 20130101; A01N 2300/00 20130101; A01N
2300/00 20130101; A01N 37/02 20130101; A01N 37/02 20130101; A01N
37/36 20130101 |
International
Class: |
A61L 2/18 20060101
A61L002/18; A01N 59/00 20060101 A01N059/00; A01N 37/36 20060101
A01N037/36; A01N 59/16 20060101 A01N059/16 |
Claims
1. A disinfectant system, comprising: a) a first chamber containing
a first solution, said first solution including an alcohol, an
organic carboxylic acid, and from 0.01 ppm, to 1,000 ppm by weight
of a transition metal or alloy thereof based on the first solution
weight content; b) a second chamber containing a second solution
including an activator; and c) a dispenser; wherein the system is
configured for mixing the first solution with the second solution
immediately before being dispensed, and wherein a peracid is formed
after mixing.
2. A system as in claim 1, wherein the system is substantially free
of chlorine and bromine-containing components.
3. A system as in claim 1, wherein the system is substantially free
of iodophore-containing components.
4. A system as in claim 1, wherein the system is substantially free
of phenolic-containing components.
5. A system as in claim 1, wherein the system is substantially free
of quaternary ammonium-containing components.
6. A system as in claim 1, wherein the alcohol in the first
solution is present in an amount of from 0.05 wt % to 10 wt %.
7. A system as in claim 1, wherein the alcohol in the first
solution is present in an amount of from 0.1 wt % to 5 wt %.
8. A system as in claim 1, wherein the alcohol in the first
solution is present in an amount of from 0.1 wt % to 1 wt %.
9. A system as in claim 1, wherein the alcohol is a
C.sub.1-C.sub.24 alcohol.
10. A system as in claim 9, wherein the C.sub.1-C.sub.24 alcohol is
selected from the group consisting of methanol, ethanol, propanol
and isopropanol, butanols, pentanols, and mixtures thereof.
11. A system as in claim 9, wherein the alcohol is ethanol.
12. A system as in 1, wherein the alcohol is a polyhydric
alcohol.
13. A system as in claim 1, wherein the alcohol is selected from
the group consisting of sorbitol, polyvinyl alcohol, ethylene
glycol, glycerin, propane-1,2-diol, and mixtures thereof.
14. A system as in claim 1, wherein the alcohol is polyvinyl
alcohol.
15. A system as in claim 1, wherein the transition metal or alloy
thereof is a Group VI to Group XI transition metal or alloy
thereof.
16. A system as in claim 1, wherein the transition metal or alloy
thereof is a Group X to Group XI transition metal or alloy
thereof.
17. A system as in claim 1, wherein the transition metal or alloy
thereof is selected from the group consisting of ruthenium,
rhodium, osmium, iridium, palladium, platinum, copper, gold,
silver, alloys thereof, and mixtures thereof.
18. A system as in claim 1, wherein the transition metal or alloy
thereof is a colloidal transition metal or alloy thereof.
19. A system as in claim 18, wherein the colloidal transition metal
is colloidal silver.
20. A system as in claim 1, wherein the transition metal or alloy
thereof is an ionic transition metal.
21. A system as in claim 1, wherein the transition metal or alloy
thereof is present at from 1 ppm to 500 ppm by weight.
22. A system as in claim 1, wherein the transition metal or allow
thereof is present at from 10 ppm to 350 ppm by weight.
23. A system as in claim 1, wherein the organic carboxylic acid is
selected from the group consisting of formic acid, acetic acid,
oxalic acid, propanoic acid, lactic acid, butanoic acid, pentanoic
acid, hexanoic acid, adipic acid, citric, benzoic acid, and
mixtures thereof.
24. A system as in claim 1, wherein the organic carboxylic acid is
citric acid.
25. A system as in claim 1, wherein the organic carboxylic acid is
acetic acid.
26. A system as in claim 1, wherein the organic carboxylic acid is
present at about 3 wt % to about 20 wt %.
27. A system as in claim 1, wherein the organic carboxylic acid is
present at about 5 wt % to about 15 wt %.
28. A system as in claim 1, wherein the organic carboxylic acid is
present at about 7 wt % to about 13 wt %.
29. A system as in claim 1, wherein the activator is present in the
second solution at from about 0.01 wt % to 10 wt %.
30. A system as in claim 1, wherein the activator is present in the
second solution at from about 0.1 to about 9 wt %.
31. A system as in claim 1, wherein the activator is present in the
second solution at from about 0.5 wt % to about 7 wt %.
32. A system as in claim 1, wherein the activator is selected from
the group consisting of methyl ethyl ketone peroxide, ozone,
potassium superoxide, lithium peroxide, barium peroxide, sodium
peroxide, calcium peroxide, strontium peroxide, magnesium peroxide,
sodium percarbonate peroxide, sodium peroxide,
carbamide(urea)peroxide, potassium peroxide, hydrogen peroxide, and
combinations thereof.
33. A system as in claim 1, wherein the activator is hydrogen
peroxide.
34. A system as in claim 1, wherein the activator is a metal
peroxide.
35. A system as in claim 1, wherein the activator is ozone.
36. A system as in claim 1, wherein the first solution and the
second solution are under pressure.
Description
[0001] This application is a continuation of U.S. patent
application Ser. No. 14/269,443 filed on May 5, 2014, which is a
continuation of U.S. patent application Ser. No. 12/617,521 filed
on Nov. 12, 2009, which claims the benefit of U.S. Provisional
Application Ser. No. 61/113,946 filed on Nov. 12, 2008, each of
which are incorporated herein by reference in their entirety.
FIELD OF THE INVENTION
[0002] The present disclosure is drawn to disinfectant systems that
can be used for a variety of purposes, including for hard surface
cleaning, and which are effective as disinfectants or even
sterilants.
BACKGROUND OF THE INVENTION
[0003] Disinfectants and sterilants, such as hard surface
disinfectants and sterilants, are widely used in both domestic and
professional settings. Exemplary of a commonly used hard surface
cleaner is Lysol.RTM. disinfectant. Though Lysol.RTM. is effective
for many applications, Lysol.RTM. is not as effective at reducing
levels of bacteria as commercially available glutaraldehyde aqueous
solutions. Glutaraldehyde aqueous solutions are widely used as
disinfectants (and often as sterilants), and are commonly available
in 1 wt % and 2 wt % solutions, particularly in medical and dental
settings. Glutaraldehyde solutions are typically used for more
delicate medical/dental instruments that would otherwise be
susceptible to damage by other sterilization methods, e.g.,
autoclaving. However, glutaraldehyde is also a powerful irritant
and respiratory sensitizer. In fact, there have been reports of
sensitization of individuals due to the fumes, which have lead to
respiratory problems, headaches, lethargy, discoloring of the skin,
etc. Because of these issues related to glutaraldehyde fumes, air
quality must often be monitored, or appropriate air ventilation
must be present. As a result, though glutaraldehyde solutions are
relatively effective disinfectants, and even sterilants, it would
be desirable to provide disinfectant systems that can exhibit
effective bacteria kill levels, and at the same time be safer for
the individuals using the disinfectant/sterilant.
SUMMARY OF THE INVENTION
[0004] It has been recognized that it would be desirable to provide
a disinfectant system that can effectively clean and disinfect
surfaces, particularly hard surfaces. In accordance with this, a
disinfectant system is provided which includes a first chamber
containing a first solution and a second chamber containing a
second solution. The first solution can include an alcohol, an
organic carboxylic acid, and from 0.01 ppm, to 1,000 ppm by weight
of a transition metal or alloy thereof based on the first solution
weight content. The second solution can include an activator. The
system is configured for mixing the first solution with the second
solution immediately before use and further includes a dispenser
through which the system is configured to dispense a mixture of the
first solution and the second solution. When the first solution and
second solution are mixed a peracid is formed.
[0005] Additional features and advantages of the invention will be
apparent from the detailed description that follows, which
illustrates, by way of example, features of the invention.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT(S)
[0006] Reference will now be made to the exemplary embodiments, and
specific language will be used herein to describe the same. It will
nevertheless be understood that no limitation of the scope of the
invention is thereby intended. Alterations and further
modifications of the inventive features illustrated herein, and
additional applications of the principles of the inventions as
illustrated herein, which would occur to one skilled in the
relevant art and having possession of this disclosure, are to be
considered within the scope of the invention. It is also to be
understood that the terminology used herein is used for the purpose
of describing particular embodiments only. The terms are not
intended to be limiting unless specified as such.
[0007] It must be noted that, as used in this specification and the
appended claims, the singular forms "a," "an," and "the" include
plural referents unless the content clearly dictates otherwise.
[0008] The term "solution" is also used throughout the
specification to describe the liquid components of the disinfectant
systems of the present disclosure. However, as these "solutions"
include colloidal transition metals, these components can also be
described as dispersions or suspensions. As the continuous phase is
typically a solution, and the transition metal is present as a
colloid, for convenience, these components will typically be
referred to as "solutions" herein.
[0009] The term "food grade" when used with respect to ingredients
or components used in the systems of the present disclosure refers
to ingredients or components that are substantially free from
ingredients which would be considered harmful or toxic to a mammal
upon consumption above levels that are generally recognized as
safe.
[0010] The term "substantially free" when used with regard to the
disinfectant systems of the present disclosure refers to the total
absence of or near total absence of a specific compound or
composition. For example, when a disinfectant system is said to be
substantially free of aldehydes, there are either no aldehydes in
the disinfectant system or only trace amounts of aldehydes in the
system.
[0011] The term "colloidal transition metals" refers to colloidal
particles of elemental transitional metals or the alloys of such
elemental transition metals. Colloidal transition metals are
distinct from salts and oxides of transition metals. Accordingly,
compounds such as silver oxide, silver nitrate, silver chloride,
silver bromide, silver iodide, and the like are not colloidal
transition metals under the present invention.
[0012] The term "activator" refers to compounds that, when allowed
to contact an organic carboxylic acid, can provide for the
generation of a peracid compound. It is noteworthy that the peracid
compound formed may have a short life-span due to the natural
degradation of peracid compounds. Non-limiting examples of
activators that can be used include methyl ethyl ketone peroxide,
ozone, hydrogen peroxide, carbamide(urea)peroxide, metal peroxides
such as potassium superoxide, lithium peroxide, barium peroxide,
sodium peroxide, calcium peroxide, strontium peroxide, magnesium
peroxide, sodium percarbonate peroxide, sodium peroxide, potassium
peroxide, other peroxide compounds, combinations thereof, and the
like.
[0013] In describing embodiments of the disinfectant systems of the
present disclosure, reference will be made to "first" or "second"
as they relate to chambers, or solutions, etc. It is noted that
these are merely relative terms, and a chamber or solution
described or shown as a "first" chamber or solution could just as
easily be referred to a "second" chamber or solution, and such
description is implicitly included herein.
[0014] Concentrations, dimensions, amounts, and other numerical
data may be presented herein in a range format. It is to be
understood that such range format is used merely for convenience
and brevity and should be interpreted flexibly to include not only
the numerical values explicitly recited as the limits of the range,
but also to include all the individual numerical values or
sub-ranges encompassed within that range as if each numerical value
and sub-range is explicitly recited. For example, a weight ratio
range of about 1 wt % to about 20 wt % should be interpreted to
include not only the explicitly recited limits of 1 wt % and about
20 wt %, but also to include individual weights such as 2 wt %, 11
wt %, 14 wt %, and sub-ranges such as 10 wt % to 20 wt %, 5 wt % to
15 wt %, etc.
[0015] In accordance with this, a disinfectant system is provided
which includes a first chamber containing a first solution and a
second chamber containing a second solution. The first solution can
include an alcohol, an organic carboxylic acid, and from 0.01 ppm,
to 1,000 ppm by weight of a transition metal or alloy thereof based
on the first solution weight content. The second solution can
include an activator. The system further includes a dispenser
through which the system is configured to dispense the first
solution and the second solution, the solutions being mixed
immediately before their use or application. Once the first
solution and second solution are mixed, a peracid can be
formed.
[0016] The disinfectant system of the present disclosure can take
on any two-chambered configuration so long as the contents of the
two chambers, namely the first solution and the second solution,
remain separate and apart until the disinfectant is needed. In one
embodiment, the system first and second chamber can both be
pressurized. The pressurization of the chambers can allow for the
solutions present in the chambers to be expelled rapidly and mixed
as they are dispensed. The mixing can occur as a function of the
two liquids coming together in the dispenser or the mixing can
occur in a mixing chamber placed in the system through which both
solutions would pass on their way to the dispenser. The system of
the present disclosure can use any mixing mechanism known in the
art so long as the mixing occurs immediately before application of
the disinfectant.
[0017] As shown in the examples, the disinfectant system of the
present disclosure can be used effectively against a wide array of
bacteria. Without being limited by theory, it is believed that the
extraordinary effectiveness of the system as a disinfectant is due,
at least in part, to the momentary formation of peracids when the
first solution and second solution of the system are mixed and
dispensed through the system dispenser. For example, in a system
that utilizes acetic acid, the addition of an activator, such as
hydrogen peroxide or others described herein, from the second
solution can result in a reaction in which peracetic acid and water
are produced in equilibrium as follows:
H.sub.2O.sub.2+CH.sub.3COOHCH.sub.3COO--OH+H.sub.2O
Once formed and dispensed on the surface, it is believed that the
peracids aid in disinfecting the surface in a rapid and effective
manner before they break down or are used up. By waiting to combine
the two solutions of the disinfectant system of the present
disclosure until just before application, the peracids are just
formed or are forming as the mixed solutions contact the target
surface. It is believed that if the solutions were combined
significantly before application to the surface, the peracids would
not be present, or at least not in significant enough
concentrations, and that the effectiveness of the solutions would
not be equivalent to that of the present system.
[0018] As disclosed above, the first solution present in the first
chamber of the system can include an alcohol, transition metal, and
an organic carboxylic acid. The alcohol present in the first
solution can be a single alcohol or a combination of multiple
alcohols. One example of alcohols which can be used in the first
solution are aliphatic alcohols and other carbon-containing
alcohols, having from 1 to 24 carbons (C.sub.1-C.sub.24 alcohol),
It is to be noted that "C.sub.1-C.sub.24 alcohol" does not
necessarily imply only straight chain saturated aliphatic alcohols,
as other carbon-containing alcohols can also be used within this
definition, including branched aliphatic alcohols, alicyclic
alcohols, aromatic alcohols, unsaturated alcohols, as well as
substituted aliphatic, alicyclic, aromatic, and unsaturated
alcohols, etc. In one embodiment, the aliphatic alcohols can be
C.sub.1 to C.sub.5 alcohols including methanol, ethanol, propanol
and isopropanol, butanols, and pentanols, due to their availability
and lower boiling points. Polyhydric alcohols can also be used
alone or in combination with other alcohols. Non-limiting examples
of polyhydric alcohols which can be used in the present disclosure
include but are not limited to ethylene glycol
(ethane-1,2-diol)glycerin (or glycerol, propane-1,2,3-triol),
propane-1,2-diol, polyvinyl alcohol, sorbitol, other polyols, and
the like. Other non-aliphatic alcohols may also be used including
but not limited to phenols and substituted phenols, erucyl alcohol,
ricinolyl alcohol, arachidyl alcohol, capryl alcohol, capric
alcohol, behenyl alcohol, lauryl alcohol (1-dodecanol), myristyl
alcohol (1-tetradecanol), cetyl (or palmityl) alcohol
(1-hexadecanol), stearyl alcohol (1-octadecanol), isostearyl
alcohol, oleyl alcohol (cis-9-octadecen-1-ol), palmitoleyl alcohol,
linoleyl alcohol (9Z,12Z-octadecadien-1-ol), elaidyl alcohol
(9E-octadecen-1-ol), elaidolinoleyl alcohol
(9E,12E-octadecadien-1-ol), linolenyl alcohol
(9Z,12Z,15Z-octadecatrien-1-ol), elaidolinolenyl alcohol
(9E,12E,15-E-octadecatrien-1-ol), combinations thereof and the
like.
[0019] In some embodiments, for practical considerations, methanol,
ethanol, and denatured alcohols (mixtures of ethanol and smaller
amounts of methanol, and optionally, minute amounts of benzene,
ketones, acetates, etc.) can often be preferred for use because of
their availability and cost. If the desire is to provide a food
grade or food safe system, then alcohols can be selected that
satisfy this requirement. The concentrations of the alcohol in the
first solution of the system can be from about 0.05 wt % to about
10 wt %. In one embodiment the concentration of the alcohol in the
first solution is about 0.1 wt % to about 5 wt %. In another
embodiment, the concentration of the alcohol in the first solution
is about 0.1 wt % to about 1 wt %.
[0020] Regarding the transition metal, in accordance with the
embodiments of the present disclosure, the metal can be in ionic
form (e.g. a metal salt) and/or colloidal form, i.e. elemental
colloids or colloids of metal alloys. In one specific embodiment,
the transition metal can be in a sub-micron form (i.e. dispersion
of less than 1 .mu.m metal colloidal particles). However, larger
colloidal transition metal particles can also be used in certain
applications. Typical transition metals that are desirable for use
include Group VI to Group XI transition metals, and more
preferably, can include Group X to Group XI transition metals.
Alloys including at least one metal from the Group VI to Group XI
metals can also be used. Further, when colloidal metals are
dispersed in a colloidal solution, there is often an amount of the
metal in ionic or salt form that is also present in the suspension
solution. For example, colloidal silver may include a certain
percentage of a silver salt or ionic silver in solution, e.g., 10%
to 90% by weight of metal content can be ionic based on the total
metal content.
[0021] This being stated, certain preferred metals for use in
accordance with embodiments of the present disclosure are
ruthenium, rhodium, osmium, iridium, palladium, platinum, copper,
gold, silver, alloys thereof, and mixtures thereof. Silver is often
the most preferred, depending on the application, the levels of
kill that are desired or required, the type of pathogen being
targeted, the substrate that is being cleaned, etc. Any of these
embodiments can also benefit from the use of alloys. For Example,
certain combinations of metals in an alloy may provide an
acceptable kill level for a specific pathogen, and also provide
benefits that are related more to secondary consideration, such as
solution stability, substrate to be cleaned, etc. Preferred
examples of transition metal alloys for use in the present
disclosure include but are not limited to copper-silver allows,
silver-manganese alloys, iron-copper alloys, chromium-silver
alloys, gold-silver alloys, and magnesium-silver alloys.
[0022] The transition metal or alloy thereof can be present in the
first solution at a concentration of about 0.01 ppm, to about 1,000
ppm. In one embodiment, the transition metal or alloy thereof can
be present in the first solution at about 1 ppm to about 500 ppm.
In yet another embodiment, the transition metal or alloy thereof
can be present in the first solution at about 10 ppm to 350 ppm by
weight.
[0023] The organic acid present in the first solution of the
disinfectant system can generally be any organic acid which can
effectively form a peracid which is effective as a disinfecting
agent. Non-limiting examples of acids which can be used include
formic acid, acetic acid, oxalic acid, propanoic acid, lactic acid,
butanoic acid, pentanoic acid, hexanoic acid, adipic acid, citric,
benzoic acid, and mixtures thereof. In one embodiment, the organic
carboxylic acid is citric acid. In another embodiment, the organic
carboxylic acid is acetic acid. The organic carboxylic acid can be
present in the first solution at about 3 wt % to about 20 wt %. In
one embodiment, the organic carboxylic acid can be present in the
first solution at about 5 wt % to about 15 wt %. In yet another
embodiment, the organic carboxylic acid can be present in the first
solution at about 7 wt % to about 13 wt %.
[0024] The first solution of the disinfectant system of the present
disclosure can have any type of liquid carrier system known in the
art. Generally, the liquid carrier will be largely aqueous,
although water need not comprise the majority of the carrier.
[0025] The second solution of the disinfectant system of the
present disclosure can include an aqueous solution of and an
activator, such as hydrogen peroxide, a metal peroxide, ozone, etc.
The activator may be present in the second solution at from about
0.01 wt % to about 10 wt %. In another embodiment, the activator
can be present in the second solution at from about 0.1 to about 9
wt %. In yet another embodiment, the activator is present in the
second solution at from about 0.5 wt % to about 7 wt %.
[0026] In one embodiment of the system of the present disclosure,
the disinfectant system can be substantially free of non-food-grade
or food safe ingredients. For example, though not required, the
disinfectant system can be substantially free of ingredients
commonly present in many commercially available surface cleaners.
Examples of non-food-grade ingredients which can be omitted from
the disinfectants or sterilants of the present disclosure include,
but are not limited to, aldehydes such as glutaraldehyde; chlorine
and bromine-containing components, iodophore-containing components,
phenolic-containing components, quaternary ammonium-containing
disinfectants; and the like.
EXAMPLES
[0027] The following examples illustrate the embodiments of the
invention that are presently best known. However, it is to be
understood that the following are only exemplary or illustrative of
the application of the principles of the present invention.
Numerous modifications and alternative compositions, methods, and
systems may be devised by those skilled in the art without
departing from the spirit and scope of the present invention. The
appended claims are intended to cover such modifications and
arrangements. Thus, while the present invention has been described
above with particularity, the following examples provide further
detail in connection with what are presently deemed to be the most
practical and preferred embodiments of the invention.
Example 1
Preparation of Disinfectant System
[0028] An aqueous disinfectant system is prepared in accordance
with embodiments of the present disclosure, which includes the
following ingredients in approximate amounts:
[0029] First Solution: [0030] 10 wt % citric acid [0031] 0.05%
polyvinyl alcohol [0032] Balance 150 ppm silver colloids in
water
[0033] Second Solution: [0034] 5 wt % hydrogen peroxide in water
The first solution and the second solution were each placed in
separate chambers from which they could be withdrawn and mixed
through a dispenser.
Example 2
Preparation of Disinfectant System
[0035] An aqueous disinfectant system is prepared in accordance
with embodiments of the present disclosure, which includes the
following ingredients in approximate amounts:
[0036] First Solution: [0037] 10 wt % citric acid [0038] 0.05%
polyvinyl alcohol [0039] Balance 300 ppm silver colloid in
water
[0040] Second Solution: [0041] 5 wt % hydrogen peroxide in water
The first solution and the second solution were each placed in
separate chambers from which they could be withdrawn and mixed
through a dispenser.
Example 3
Kill-Time Studies of Staphylococcus aureus Using Disinfectants of
Examples 1 and 2
[0042] A study was conducted to determine the antimicrobial
activity of the disinfectant systems of Example 1 and Example 2
when challenged with an organic load, on the test organism
Staphylococcus aureus. This was accomplished by performing a
standard AOAC Germicidal Spray Product as Disinfectants Protocol;
Method 961.02 using test organisms dried onto glass slides which
were sprayed 3 seconds and held for 1, 5, and 10 minutes prior to
subculture.
[0043] Specifically, the test suspension was prepared by growing a
culture of Staphylococcus aureus, ATCC 6538, in Nutrient Broth at
37.degree. C., for 48 hour. A 10 .mu.aliquot of the test culture
was transferred to 1''.times.1'' square glass slides in Petri
dishes using a calibrated micropipette. The inoculum was
immediately spread evenly over the entire surface of the slide. The
dish was covered, and the process was repeated until 10 slides per
time point were inoculated.
[0044] The slides were then dried in a 37.degree. C. incubator for
30-40 minutes. The slides with S. aureus, 10 per Example 1 and
Example 2 per time period (60 total), were sprayed with the test
disinfectant using the system of Examples 1 and 2. The slides were
sprayed for 3 seconds at a distance of about 6-8 inches from the
slides. The slides were held for 1, 5, and 10 minutes, and then
excess liquid was drained from the slides. The slides were then
transferred to individual 32.times.200 mm glass culture tubes
containing 20 ml Fluid Thioglycollate broth and shaken thoroughly.
All of the tubes were incubated at 37.degree. C. for 48 hours. The
tubes were then observed for growth (+) or no growth (-) by visual
turbidity.
[0045] As a control two inoculated, but unsprayed slides prepared
as described above were used. A media control containing no slide
was also included. To check for bacteriostasis, a few
representative negative subculture tubes are evaluated for residual
disinfectant bacteriostasis by inoculating each with a small look
contaminated with a respective test culture suspension and
re-incubating the tubes at 37.degree. C. for 24 hours.
[0046] The results of the test are provided as follows:
TABLE-US-00001 TABLE 1 Test Results after 1 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00002 TABLE 2 Test Results after 5 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00003 TABLE 3 Test Results after 10 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00004 TABLE 4 control Growth Sample (= or -) S. Aureus
positive control 1 + S. Aureus positive control 2 + Media Control
-
TABLE-US-00005 TABLE 5 Bacteriostasis controls Growth Tube (+ or -)
Spray 1, 1-min Tube 1 + Spray 1, 1-min, Tube 2 + Spray 1, 5-min
Tube 1 + Spray 1, 5-min Tube 2 + Spray 1, 10-min Tube 1 + Spray 1,
10-min Tube 2 + Spray 2, 1-min Tube 1 + Spray 2, 1-min Tube 2 +
Spray 2, 5-min Tube 1 + Spray 2, 5-min Tube 2 + Spray 2, 10-min
Tube 1 + Spray 2, 10-min Tube 2 +
[0047] All controls produced the expected results. The positive
control slides that were not treated with disinfectant showed
characteristic growth for S. aureus. The media control tube with no
slide showed no growth. The re-inoculated bacteriostasis control
tubes all showed positive growth upon re-incubation, indicating no
bacteriostasis from the residual disinfectant left on the
slides.
[0048] The two-part systems of Example 1 and 2 were able to
completely kill the dried organisms on all of the slides for all of
the time points. Since killing of ten out of ten slides is a
required to pass the AOAC Germicidal Spray test, the systems passed
the test at the 1, 5, and 10 minutes contact times.
Example 4
Kill-Time Studies of Salmonella choleraesuis Using Disinfectants of
Examples 1 and 2
[0049] A study was conducted to determine the antimicrobial
activity of the disinfectant systems of Example 1 and Example 2
when challenged with an organic load, on the test organism
Salmonella choleraesuis. This was accomplished by performing a
standard AOAC Germicidal Spray Product as Disinfectants Protocol;
Method 961.02 using test organisms dried onto glass slides which
were sprayed 3 seconds and held for 1, 5, and 10 minutes prior to
subculture.
[0050] Specifically, the test suspension was prepared by growing a
culture of Salmonella choleraesuis, ATCC 10708, in Nutrient Broth
at 37.degree. C., for 48 hour. A 10 .mu.aliquot of the test culture
was transferred to 1''.times.1'' square glass slides in Petri
dishes using a calibrated micropipette. The inoculum was
immediately spread evenly over the entire surface of the slide. The
dish was covered, and the process was repeated until 10 slides per
time point were inoculated.
[0051] The slides were then dried in a 37.degree. C. incubator for
30-40 minutes. The slides with Salmonella choleraesuis, 10 per
Example 1 and Example 2 per time period (60 total), were sprayed
with the test disinfectant using the system of Examples 1 and 2.
The slides were sprayed for 3 seconds at a distance of about 6-8
inches from the slides. The slides were held for 1, 5, and 10
minutes, and then excess liquid was drained from the slides. The
slides were then transferred to individual 32.times.200 mm glass
culture tubes containing 20 ml Fluid Thioglycollate broth and
shaken thoroughly. All of the tubes were incubated at 37.degree. C.
for 48 hours. The tubes were then observed for growth (+) or no
growth (-) by visual turbidity.
[0052] As a control two inoculated, but unsprayed slides prepared
as described above were used. A media control containing no slide
was also included. To check for bacteriostasis, a few
representative negative subculture tubes are evaluated for residual
disinfectant bacteriostasis by inoculating each with a small look
contaminated with a respective test culture suspension and
re-incubating the tubes at 37.degree. C. for 24 hours.
[0053] The results of the test are provided as follows:
TABLE-US-00006 TABLE 6 Test Results after 1 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00007 TABLE 7 Test Results after 5 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00008 TABLE 8 Test Results after 10 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00009 TABLE 9 control Growth Sample (= or -) Salmonella
choleraesuis + positive control 1 Salmonella choleraesuis +
positive control 2 Media Control 1 - Media Control 2 -
TABLE-US-00010 TABLE 10 Bacteriostasis controls Growth Tube (+ or
-) Spray 1, 1-min Tube 1 + Spray 1, 5-min Tube 1 + Spray 1, 10-min
Tube 1 + Spray 2, 1-min Tube 1 + Spray 2, 5-min Tube 1 + Spray 2,
10-min Tube 1 +
[0054] All controls produced the expected results. The positive
control slides that were not treated with disinfectant showed
characteristic growth for Salmonella choleraesuis. The media
control tube with no slide showed no growth. The re-inoculated
bacteriostasis control tubes all showed positive growth upon
re-incubation, indicating no bacteriostasis from the residual
disinfectant left on the slides.
[0055] The two-part systems of Example 1 and 2 were able to
completely kill the dried organisms on all of the slides for all of
the time points. Since killing of ten out of ten slides is a
required to pass the AOAC Germicidal Spray test, the systems passed
the test at the 1, 5, and 10 minutes contact times.
Example 5
Kill-Time Studies of Pseudomonas aeruginosa Using Disinfectants of
Examples 1 and 2
[0056] A study was conducted to determine the antimicrobial
activity of the disinfectant systems of Example 1 and Example 2
when challenged with an organic load, on the test organism
Pseudomonas aeruginosa. This was accomplished by performing a
standard AOAC Germicidal Spray Product as Disinfectants Protocol;
Method 961.02 using test organisms dried onto glass slides which
were sprayed 3 seconds and held for 1, 5, and 10 minutes prior to
subculture.
[0057] Specifically, the test suspension was prepared by growing a
culture of Pseudomonas aeruginosa, ATCC 10708, in Nutrient Broth at
37.degree. C., for 48 hour. A 10 .mu.aliquot of the test culture
was transferred to 1''.times.1'' square glass slides in Petri
dishes using a calibrated micropipette. The inoculum was
immediately spread evenly over the entire surface of the slide. The
dish was covered, and the process was repeated until 10 slides per
time point were inoculated.
[0058] The slides were then dried in a 37.degree. C. incubator for
30-40 minutes. The slides with Pseudomonas aeruginosa, 10 per
Example 1 and Example 2 per time period (60 total), were sprayed
with the test disinfectant using the system of Examples 1 and 2.
The slides were sprayed for 3 seconds at a distance of about 6-8
inches from the slides. The slides were held for 1, 5, and 10
minutes, and then excess liquid was drained from the slides. The
slides were then transferred to individual 32.times.200 mm glass
culture tubes containing 20 ml Fluid Thioglycollate broth and
shaken thoroughly. All of the tubes were incubated at 37.degree. C.
for 48 hours. The tubes were then observed for growth (+) or no
growth (-) by visual turbidity.
[0059] As a control two inoculated, but unsprayed slides prepared
as described above were used. A media control containing no slide
was also included. To check for bacteriostasis, a few
representative negative subculture tubes are evaluated for residual
disinfectant bacteriostasis by inoculating each with a small look
contaminated with a respective test culture suspension and
re-incubating the tubes at 37.degree. C. for 24 hours.
[0060] The results of the test are provided as follows:
TABLE-US-00011 TABLE 11 Test Results after 1 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00012 TABLE 12 Test Results after 5 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00013 TABLE 13 Test Results after 10 minute contact time
Tube Example 1 Example 2 number Growth (+ or -) Growth (+ or -) 1 -
- 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - -
TABLE-US-00014 TABLE 14 control Growth Sample (= or -) Pseudomonas
aeruginosa + positive control 1 Pseudomonas aeruginosa + positive
control 2 Media Control 1 - Media Control 2 -
TABLE-US-00015 TABLE 15 Bacteriostasis controls Growth Tube (+ or
-) Spray 1, 1-min Tube 1 + Spray 1, 5-min Tube 1 + Spray 1, 10-min
Tube 1 + Spray 2, 1-min Tube 1 + Spray 2, 5-min Tube 1 + Spray 2,
10-min Tube 1 +
[0061] All controls produced the expected results. The positive
control slides that were not treated with disinfectant showed
characteristic growth for Pseudomonas aeruginosa. The media control
tube with no slide showed no growth. The re-inoculated
bacteriostasis control tubes all showed positive growth upon
re-incubation, indicating no bacteriostasis from the residual
disinfectant left on the slides.
[0062] The two-part systems of Example 1 and 2 were able to
completely kill the dried organisms on all of the slides for all of
the time points. Since killing of ten out of ten slides is a
required to pass the AOAC Germicidal Spray test, the systems passed
the test at the 1, 5, and 10 minutes contact times.
Example 6
Kill-Time Studies of Methicillin-Resistant Staphylococcus aureus
(MRSA) Using Disinfectants of Example 2
[0063] A study was conducted to determine the antimicrobial
activity of the disinfectant system of Example 2 when challenged
with an organic load, on the test organism Methicillin-resistant
Staphylococcus aureus (MRSA). This was accomplished by performing a
standard AOAC Germicidal Spray Product as Disinfectants Protocol;
Method 961.02 using test organisms dried onto glass slides which
were sprayed 3 seconds and held for 1, 5, and 10 minutes prior to
subculture.
[0064] Specifically, the test suspension was prepared by growing a
culture of Methicillin-resistant Staphylococcus aureus (MRSA), ATCC
43301, in Nutrient Broth at 37.degree. C., for 48 hour. A 10
.mu.aliquot of the test culture was transferred to 1''.times.1''
square glass slides in Petri dishes using a calibrated
micropipette. The inoculum was immediately spread evenly over the
entire surface of the slide. The dish was covered, and the process
was repeated until 10 slides per time point were inoculated.
[0065] The slides were then dried in a 37.degree. C. incubator for
30-40 minutes. The slides with MRSA, 10 per Example 2 per time
period (60 total), were sprayed with the test disinfectant using
the system of Example 2. The slides were sprayed for 3 seconds at a
distance of about 6-8 inches from the slides. The slides were held
for 1, 5, and 10 minutes, and then excess liquid was drained from
the slides. The slides were then transferred to individual
32.times.200 mm glass culture tubes containing 20 ml Fluid
Thioglycollate broth and shaken thoroughly. All of the tubes were
incubated at 37.degree. C. for 48 hours. The tubes were then
observed for growth (+) or no growth (-) by visual turbidity.
[0066] As a control two inoculated, but unsprayed slides prepared
as described above were used. A media control containing no slide
was also included. To check for bacteriostasis, a few
representative negative subculture tubes are evaluated for residual
disinfectant bacteriostasis by inoculating each with a small look
contaminated with a respective test culture suspension and
re-incubating the tubes at 37.degree. C. for 24 hours.
[0067] The results of the test are provided as follows:
TABLE-US-00016 TABLE 16 Test Results after 1 minute contact time
Tube Example 2 number Growth (+ or -) 1 - 2 - 3 - 4 - 5 - 6 - 7 - 8
- 9 - 10 -
TABLE-US-00017 TABLE 17 Test Results after 5 minute contact time
Tube Example 2 number Growth (+ or -) 1 - 2 - 3 - 4 - 5 - 6 - 7 - 8
- 9 - 10 -
TABLE-US-00018 TABLE 18 Test Results after 10 minute contact time
Tube Example 2 number Growth (+ or -) 1 - 2 - 3 - 4 - 5 - 6 - 7 - 8
- 9 - 10 -
TABLE-US-00019 TABLE 19 control Growth Sample (= or -) MRSA
positive control 1 + MRSA positive control 2 + Media Control 1
-
TABLE-US-00020 TABLE 20 Bacteriostasis controls Growth Tube (+ or
-) Spray 2, 1-min Tube 1 + Spray 2, 5-min Tube 1 + Spray 2, 10-min
Tube 1 +
[0068] All controls produced the expected results. The positive
control slides that were not treated with disinfectant showed
characteristic growth for MRSA. The media control tube with no
slide showed no growth. The re-inoculated bacteriostasis control
tubes all showed positive growth upon re-incubation, indicating no
bacteriostasis from the residual disinfectant left on the
slides.
[0069] The two-part system of Example 2 was able to completely kill
the dried organisms on all of the slides for all of the time
points. Since killing of ten out of ten slides is a required to
pass the AOAC Germicidal Spray test, the systems passed the test at
the 1, 5, and 10 minutes contact times.
Example 7
Preparation of Disinfectant System
[0070] An aqueous disinfectant system is prepared in accordance
with embodiments of the present disclosure, which includes the
following ingredients in approximate amounts:
[0071] First Solution: [0072] 10 wt % acetic acid [0073] 0.05%
polyvinyl alcohol [0074] Balance 200 ppm silver colloids in
water
[0075] Second Solution: [0076] 7 wt % magnesium peroxide in water
The first solution and the second solution were each placed in
separate chambers from which they could be withdrawn and mixed
through a dispenser.
Example 8
Preparation of Disinfectant System
[0077] An aqueous disinfectant system is prepared in accordance
with embodiments of the present disclosure, which includes the
following ingredients in approximate amounts:
[0078] First Solution: [0079] 8 wt % citric acid [0080] 2%
polyvinyl alcohol [0081] Balance 300 ppm silver colloid in
water
[0082] Second Solution: [0083] 5 wt % sodium percarbonate in water
The first solution and the second solution were each placed in
separate chambers from which they could be withdrawn and mixed
through a dispenser.
[0084] While the invention has been described with reference to
certain preferred embodiments, those skilled in the art will
appreciate that various modifications, changes, omissions, and
substitutions can be made without departing from the spirit of the
invention. It is therefore intended that the invention be limited
only by the scope of the appended claims.
* * * * *