U.S. patent application number 14/619870 was filed with the patent office on 2015-08-06 for delta-5 desaturase and its use in making polyunsaturated fatty acids. This patent application is currently assigned to E. I. DU PONT DE NEMOURS AND COMPANY. The applicant listed for this patent is E. I. du Pont de Nemours and Company. Invention is credited to Howard G. DAMUDE, Quinn Qun ZHU.
Application Number | 20150216210 14/619870 |
Document ID | / |
Family ID | 38723816 |
Filed Date | 2015-08-06 |
United States Patent Application | 20150216210 |
Kind Code | A1 |
DAMUDE; Howard G. ; et al. | August 6, 2015 |
Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-5 desaturase in plants and oleaginous yeast are disclosed.
Inventors: | DAMUDE; Howard G.; (Hockessin, DE) ; ZHU; Quinn Qun; (West Chester, PA) | ||||||||||
Applicant: |
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Assignee: | E. I. DU PONT DE NEMOURS AND
COMPANY Wilmington DE |
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Family ID: | 38723816 | ||||||||||
Appl. No.: | 14/619870 | ||||||||||
Filed: | February 11, 2015 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
11749889 | May 17, 2007 | 8962917 | ||
14619870 | ||||
60801172 | May 17, 2006 | |||
Current U.S. Class: | 426/623 ; 426/618; 426/629; 426/634 |
Current CPC Class: | C12Y 114/19 20130101; A23K 10/30 20160501; A23L 7/196 20160801; A23V 2002/00 20130101; C12P 7/6472 20130101; A23L 19/03 20160801; C12P 7/6427 20130101; C12N 9/0083 20130101; C12N 9/0071 20130101 |
International Class: | A23L 1/182 20060101 A23L001/182; A23K 1/14 20060101 A23K001/14; C12N 9/02 20060101 C12N009/02; A23L 1/212 20060101 A23L001/212 |
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 94 <210> SEQ ID NO 1 <211> LENGTH: 1350
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(1350) <223> OTHER INFORMATION: delta-5
desaturase <400> SEQUENCE: 1 atggctctca gtcttaccac agaacagctg
ttagaacgcc ctgatttggt tgcgattgat 60 ggcatcctct acgaccttga
agggcttgcc aaagttcatc caggaggaga tttgattctc 120 gcttctggtg
cctctgatgc ctcccctctc ttttattcaa tgcatccata cgtcaaaccg 180
gagaattcca aattgcttca acagttcgtc cgagggaagc atgaccgcac ctcgaaggac
240 attgtctaca cgtatgattc tcccttcgca caagacgtta agcggacaat
gcgcgaggtg 300 atgaaaggga ggaactggta cgcaacccct ggcttctggc
tgcgcaccgt tgggatcatc 360 gccgtgacgg ccttttgcga gtggcactgg
gctaccacgg ggatggtgct gtggggcctg 420 ttgactggat tcatgcacat
gcagatcggc ttatccatcc agcatgatgc gtcccacggg 480 gccatcagca
agaagccttg ggtcaacgcc ctcttcgcct acggcattga cgtcatcgga 540
tcgtcccggt ggatttggct gcagtcgcac atcatgcggc accacaccta caccaaccag
600 cacggcctcg acctggatgc ggagtcggca gagccgttcc tggtgttcca
caactacccc 660 gccgcaaaca ccgcccgaaa gtggttccac cgcttccaag
cttggtacat gtaccttgtg 720 ctgggggcat acggggtatc gctggtgtac
aacccgctct acattttccg gatgcagcac 780 aatgacacca tcccagagtc
tgtcacggcc atgcgggaga atggctttct gcggcgctac 840 cgcacacttg
cattcgtgat gcgagctttc ttcatcttcc ggaccgcatt cttgccctgg 900
tacctcactg ggacctcatt gctgatcacc attcctctgg tgcccactgc aactggtgcc
960 ttcttgacgt tcttcttcat tttgtcccac aattttgatg gctccgaacg
gatccccgac 1020 aagaactgca aggttaagag ctctgagaag gacgttgagg
ctgaccaaat tgactggtat 1080 cgggcgcagg tggagacgtc ctccacatac
ggtggcccca tcgccatgtt cttcactggc 1140 ggtctcaatt tccagatcga
gcaccacctc tttccccgga tgtcgtcttg gcactacccc 1200 ttcgtccagc
aggcggtccg ggagtgttgc gaacgccatg gagtgcgata tgttttctac 1260
cctaccatcg tcggcaacat catctccacc ctgaagtaca tgcataaggt gggtgtcgtc
1320 cactgcgtga aggacgcaca ggattcctga 1350 <210> SEQ ID NO 2
<211> LENGTH: 449 <212> TYPE: PRT <213> ORGANISM:
Euglena gracilis <400> SEQUENCE: 2 Met Ala Leu Ser Leu Thr
Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 1 5 10 15 Val Ala Ile Asp
Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 20 25 30 His Pro
Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 35 40 45
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 50
55 60 Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys
Asp 65 70 75 80 Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val
Lys Arg Thr 85 90 95 Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr
Ala Thr Pro Gly Phe 100 105 110 Trp Leu Arg Thr Val Gly Ile Ile Ala
Val Thr Ala Phe Cys Glu Trp 115 120 125 His Trp Ala Thr Thr Gly Met
Val Leu Trp Gly Leu Leu Thr Gly Phe 130 135 140 Met His Met Gln Ile
Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 145 150 155 160 Ala Ile
Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 165 170 175
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 180
185 190 Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala
Glu 195 200 205 Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala
Ala Asn Thr 210 215 220 Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp
Tyr Met Tyr Leu Val 225 230 235 240 Leu Gly Ala Tyr Gly Val Ser Leu
Val Tyr Asn Pro Leu Tyr Ile Phe 245 250 255 Arg Met Gln His Asn Asp
Thr Ile Pro Glu Ser Val Thr Ala Met Arg 260 265 270 Glu Asn Gly Phe
Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 275 280 285 Ala Phe
Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 290 295 300
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 305
310 315 320 Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly
Ser Glu 325 330 335 Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser
Glu Lys Asp Val 340 345 350 Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala
Gln Val Glu Thr Ser Ser 355 360 365 Thr Tyr Gly Gly Pro Ile Ala Met
Phe Phe Thr Gly Gly Leu Asn Phe 370 375 380 Gln Ile Glu His His Leu
Phe Pro Arg Met Ser Ser Trp His Tyr Pro 385 390 395 400 Phe Val Gln
Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 405 410 415 Tyr
Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 420 425
430 Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp
435 440 445 Ser <210> SEQ ID NO 3 <211> LENGTH: 1350
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <223>
OTHER INFORMATION: synthetic delta-5 desaturase (codon-optimized)
for Yarrowia lipolytica <400> SEQUENCE: 3 atggctctct
cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac 60
ggcattctct acgatctgga aggtcttgcc aaggtccatc ccggaggcga cttgatcctc
120 gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta
cgtcaagccc 180 gagaactcga agctgcttca acagttcgtg cgaggcaagc
acgaccgaac ctccaaggac 240 attgtctaca cctacgactc tccctttgca
caggacgtca agcgaactat gcgagaggtc 300 atgaaaggtc ggaactggta
tgccacacct ggattctggc tgcgaaccgt tggcatcatt 360 gctgtcaccg
ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc 420
ttgactggat tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt
480 gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga
cgtcattgga 540 tcgtccagat ggatctggct gcagtctcac atcatgcgac
atcacaccta caccaatcag 600 catggtctcg acctggatgc cgagtccgca
gaaccattcc ttgtgttcca caactaccct 660 gctgccaaca ctgctcgaaa
gtggtttcac cgattccagg cctggtacat gtacctcgtg 720 cttggagcct
acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac 780
aacgacacca ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac
840 cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt
cttgccctgg 900 tatctcactg gaacctccct gctcatcacc attcctctgg
tgcccactgc taccggtgcc 960 ttcctcacct tctttttcat cttgtctcac
aacttcgatg gctcggagcg aatccccgac 1020 aagaactgca aggtcaagag
ctccgagaag gacgttgaag ccgatcagat cgactggtac 1080 agagctcagg
tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc 1140
ggtctcaact tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc
1200 ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta
cgtcttctac 1260 cctaccattg tgggcaacat catttccacc ctcaagtaca
tgcacaaagt cggtgtggtt 1320 cactgtgtca aggacgctca ggattcctaa 1350
<210> SEQ ID NO 4 <211> LENGTH: 590 <212> TYPE:
DNA <213> ORGANISM: Euglena gracilis <400> SEQUENCE: 4
ggccaccaca tctacaccaa ccagcacggc ctcgacctgg atgcggagtc ggcagagccg
60 ttcctggtgt tccacaacta ccccgccgca aacaccgccc gaaagtggtt
ccaccgcttc 120 caagcttggt acatgtacct tgtgctgggg gcatacgggg
tatcgctggt gtacaacccg 180 ctctacattt tccggatgca gcacaatgac
accatcccag agtctgtcac ggccatgcgg 240 gaaaatggct ttctgcggcg
ctaccgcaca cttgcattcg tgatgcgagc tttcttcatc 300 ttccggaccg
cattcttgcc ctggtacctc actgggacct cattgctgat caccattcct 360
ctggtgccca ccgcaactgg tgccttcttg acgttcttct tcattttgtc ccacaatttt
420 gatggctccg aacggatccc cgacaagaac tgcaaggtta agagctctga
gaaggacgtt 480 gaggctgacc aaattgactg gtatcgggcg caggtggaga
cgtcctccac atacggtggc 540 cccatcgcca tgttcttcac tggcggtctc
aactaccaaa tcgtccacca 590 <210> SEQ ID NO 5 <211>
LENGTH: 196 <212> TYPE: PRT <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 5 Gly His His Ile Tyr Thr Asn Gln
His Gly Leu Asp Leu Asp Ala Glu 1 5 10 15 Ser Ala Glu Pro Phe Leu
Val Phe His Asn Tyr Pro Ala Ala Asn Thr 20 25 30 Ala Arg Lys Trp
Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 35 40 45 Leu Gly
Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 50 55 60
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 65
70 75 80 Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val
Met Arg 85 90 95 Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp
Tyr Leu Thr Gly 100 105 110 Thr Ser Leu Leu Ile Thr Ile Pro Leu Val
Pro Thr Ala Thr Gly Ala 115 120 125 Phe Leu Thr Phe Phe Phe Ile Leu
Ser His Asn Phe Asp Gly Ser Glu 130 135 140 Arg Ile Pro Asp Lys Asn
Cys Lys Val Lys Ser Ser Glu Lys Asp Val 145 150 155 160 Glu Ala Asp
Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 165 170 175 Thr
Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Tyr 180 185
190 Gln Ile Val His 195 <210> SEQ ID NO 6 <211> LENGTH:
797 <212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<400> SEQUENCE: 6 cacagaacag ctgttagaac gccctgattt ggttgcgatt
gatggcatcc tctacgacct 60 tgaagggctt gccaaagttc atccaggagg
agatttgatt ctcgcttctg gtgcctctga 120 tgcctcccct ctcttttatt
caatgcatcc atacgtcaaa ccggagaatt ccaaattgct 180 tcaacagttc
gtccgaggga agcatgaccg cacctcgaag gacattgtct acacgtatga 240
ttctcccttc gcacaagacg ttaagcggac aatgcgcgag gtgatgaaag ggaggaactg
300 gtacgcaacc cctggcttct ggctgcgcac cgttgggatc atcgccgtga
cggccttttg 360 cgagtggcac tgggctacca cggggatggt gctgtggggc
ctgttgactg gattcatgca 420 catgcagatc ggcttatcca tccagcatga
tgcgtcccac ggggccatca gcaaggagcc 480 ttgggtcaac gccctcttcg
cctacggcat tgacgtcatc ggatcgtccc ggtggatttg 540 gctgcagtca
cacatcatgc ggcaccacac ctacaccaac cagcacggcc tcgacctgga 600
tgcggagtcg gcagagccgt tcctggtgtt ccacagctac cccgccgcaa acaccgcccg
660 aaagtggttc caccgcttcc aagcttggta catgtacctt gcgctggggg
catacggggt 720 atcgctggtg tacaacccgc tctacatttt ccggatgcag
cacaatgaca ccatcccaga 780 gtctgtcacg gccatgc 797 <210> SEQ ID
NO 7 <211> LENGTH: 559 <212> TYPE: DNA <213>
ORGANISM: Euglena gracilis <400> SEQUENCE: 7 cacagaacag
ctgttagaac gccctgattt ggttgcgatt gatggcatcc tctacgacct 60
tgaagggctt gccaaagttc atccaggagg agatttgatt ctcgcttctg gtgcctctga
120 tgcctcccct ctcttttatt caatgcatcc atacgtcaaa ccggagaatt
ccaaattgct 180 tcaacagttc gtccgaggga agcatgaccg cacctcgaag
gacattgtct acacgtatga 240 ttctcccttc gcacaagacg ttaagcggac
aatgcgcgag gtgatgaaag ggaggaactg 300 gtacgcaacc cctggcttct
ggctgcgcac cgttgggatc atcgccgtga cggccttttg 360 cgagtggcac
tgggctacca cggggatggt gctgtggggc ctgttgactg gattcatgca 420
catgcagatc ggcttatcca tccagcatga tgcgtcccac ggggccatca gcaaggagcc
480 ttgggtcaac gccctcttcg cctacggcat tgacgtcatc ggatcgtccc
ggtggatttg 540 gctgcagtca cacatcatg 559 <210> SEQ ID NO 8
<211> LENGTH: 273 <212> TYPE: DNA <213> ORGANISM:
Euglena gracilis <400> SEQUENCE: 8 ggaatggctc tcagtcttac
cacagaacag ctgttagaac gccctgattt ggttgcgatt 60 gatggcatcc
tctacgacct tgaagggctt gccaaagttc atccaggagg agatttgatt 120
ctcgcttctg gtgcctctga tgcctcccct ctcttttatt caatgcatcc atacgtcaaa
180 ccggagaatt ccaaattgct tcaacagttc gtccgaggga agcatgaccg
cacctcgaag 240 gacattgtct acacgtatga ttctcccttc gca 273 <210>
SEQ ID NO 9 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Euglena gracilis <400> SEQUENCE: 9
ggaatggctc tcagtcttac 20 <210> SEQ ID NO 10 <211>
LENGTH: 728 <212> TYPE: DNA <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 10 cctcactggg acctcattgc tgatcaccat
tcctctggtg cccacygcaa ctggtgcctt 60 cttgacgttc ttcttcattt
tgtcccacaa ttttgatggc tccgaacgga tccccgacaa 120 gaactgcaag
gttaagagct ctgagaagga cgttgaggct gaccaaattg actggtatcg 180
ggcgcaggtg gagacgtcct ccacatacgg tggccccatc gccatgttct tcactggcgg
240 tctcaatttc cagatcgagc accacctctt tccccggatg tcgtcttggc
actacccctt 300 cgtccagcag gcggtccggg agtgttgcga acgccatgga
gtgcgatatg ttttctaccc 360 taccatcgtc ggcaacatca tctccaccct
gaagtacatg cataaggtgg gtgtcgtcca 420 ctgcgtgaag gacgcacagg
attcctgagg ggcagggtga ccaagaacga tcatcgatgt 480 gttcttctgg
ccctttggtg gggtactcgc cagattgctc cactgaaccg tatcctaact 540
ctgaccctct ccaaccctgt gttgagtgtt tgctttatgc tccaaagtgg cttcttattt
600 ggtgacccgt ggggcagcgg cacctgtgcc atgcagctga taacccaggc
tgctactcta 660 agttggatgg ccaatcgtct ggcattgata tccctgctca
gcgttgcatt ccaatggttt 720 gcttatcc 728 <210> SEQ ID NO 11
<211> LENGTH: 464 <212> TYPE: DNA <213> ORGANISM:
Euglena gracilis <400> SEQUENCE: 11 cctctttccc cggatgtcgt
cttggcacta ccccttcgtc cagcaggcgg tccgggagtg 60 ttgcgaacgc
catggagtgc gatatgtttt ctaccctacc atcgtcggca acatcatctc 120
caccctgaag tacatgcata aggtgggtgt cgtccactgc gtgaaggacg cacaggattc
180 ctgaggggca gggtgaccaa gaacgatcat cgatgtgttc ttctggccct
ttggtggggt 240 actcgccaga ttgctccact gaaccgtatc ctaactctga
ccctctccaa ccctgtgttg 300 agtgtttgct ttatgctcca aagtggcttc
ttatttggtg acccgtgggg cagcggcacc 360 tgtgccatgc agctgataac
ccaggctgct actctaagtt ggatggccaa tcgtctggca 420 ttgatatccc
tgctcagcgt tgcattccaa tggtttgctt atcc 464 <210> SEQ ID NO 12
<211> LENGTH: 456 <212> TYPE: PRT <213> ORGANISM:
Pythium irregulare <220> FEATURE: GenBank Accession No.
AAL13311 <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: delta-5 desaturase <400> SEQUENCE: 12 Met Gly
Thr Asp Gln Gly Lys Thr Phe Thr Trp Gln Glu Val Ala Lys 1 5 10 15
His Asn Thr Ala Lys Ser Ala Trp Val Ile Ile Arg Gly Glu Val Tyr 20
25 30 Asp Val Thr Glu Trp Ala Asp Lys His Pro Gly Gly Ser Glu Leu
Ile 35 40 45 Val Leu His Ser Gly Arg Glu Cys Thr Asp Thr Phe Tyr
Ser Tyr His 50 55 60 Pro Phe Ser Asn Arg Ala Asp Lys Ile Leu Ala
Lys Tyr Lys Ile Gly 65 70 75 80 Lys Leu Val Gly Gly Tyr Glu Phe Pro
Val Phe Lys Pro Asp Ser Gly 85 90 95 Phe Tyr Lys Glu Cys Ser Glu
Arg Val Ala Glu Tyr Phe Lys Thr Asn 100 105 110 Asn Leu Asp Pro Lys
Ala Ala Phe Ala Gly Leu Trp Arg Met Val Phe 115 120 125 Val Phe Ala
Val Ala Ala Leu Ala Tyr Met Gly Met Asn Glu Leu Ile 130 135 140 Pro
Gly Asn Val Tyr Ala Gln Tyr Ala Trp Gly Val Val Phe Gly Val 145 150
155 160 Phe Gln Ala Leu Pro Leu Leu His Val Met His Asp Ser Ser His
Ala 165 170 175 Ala Cys Ser Ser Ser Pro Ala Met Trp Gln Ile Ile Gly
Arg Gly Val 180 185 190 Met Asp Trp Phe Ala Gly Ala Ser Met Val Ser
Trp Leu Asn Gln His 195 200 205 Val Val Gly His His Ile Tyr Thr Asn
Val Ala Gly Ala Asp Pro Asp 210 215 220 Leu Pro Val Asp Phe Glu Ser
Asp Val Arg Arg Ile Val His Arg Gln 225 230 235 240 Val Leu Leu Pro
Ile Tyr Lys Phe Gln His Ile Tyr Leu Pro Pro Leu 245 250 255 Tyr Gly
Val Leu Gly Leu Lys Phe Arg Ile Gln Asp Val Phe Glu Thr 260 265 270
Phe Val Ser Leu Thr Asn Gly Pro Val Arg Val Asn Pro His Pro Val 275
280 285 Ser Asp Trp Val Gln Met Ile Phe Ala Lys Ala Phe Trp Thr Phe
Tyr 290 295 300 Arg Ile Tyr Ile Pro Leu Val Trp Leu Lys Ile Thr Pro
Ser Thr Phe 305 310 315 320 Trp Gly Val Phe Phe Leu Ala Glu Phe Thr
Thr Gly Trp Tyr Leu Ala 325 330 335 Phe Asn Phe Gln Val Ser His Val
Ser Thr Glu Cys Glu Tyr Pro Cys 340 345 350 Gly Asp Ala Pro Ser Ala
Glu Val Gly Asp Glu Trp Ala Ile Ser Gln 355 360 365 Val Lys Ser Ser
Val Asp Tyr Ala His Gly Ser Pro Leu Ala Ala Phe 370 375 380 Leu Cys
Gly Ala Leu Asn Tyr Gln Val Thr His His Leu Tyr Pro Gly 385 390 395
400 Ile Ser Gln Tyr His Tyr Pro Ala Ile Ala Pro Ile Ile Ile Asp Val
405 410 415 Cys Lys Lys Tyr Asn Ile Lys Tyr Thr Val Leu Pro Thr Phe
Thr Glu 420 425 430 Ala Leu Leu Ala His Phe Lys His Leu Lys Asn Met
Gly Glu Leu Gly 435 440 445 Lys Pro Val Glu Ile His Met Gly 450 455
<210> SEQ ID NO 13 <211> LENGTH: 477 <212> TYPE:
PRT <213> ORGANISM: Phytophthora megasperma <220>
FEATURE: GenBank Accession No. CAD53323 <221> NAME/KEY:
MISC_FEATURE <223> OTHER INFORMATION: delta-5 desaturase
<400> SEQUENCE: 13 Met Ala Pro Ile Glu Thr Val Lys Asp Ala
Asn Glu Gly Leu His Gln 1 5 10 15 Arg Lys Gly Ala Ala Ala Ala Ser
Lys Asp Thr Thr Thr Phe Thr Trp 20 25 30 Gln Asp Val Ala Lys His
Asn Thr Ala Lys Ser Ala Trp Val Thr Ile 35 40 45 Arg Gly Val Val
Tyr Asp Val Thr Glu Trp Ala Asp Arg His Pro Gly 50 55 60 Gly Arg
Glu Leu Val Leu Leu His Ser Gly Arg Glu Cys Thr Asp Thr 65 70 75 80
Phe Asp Ser Tyr His Pro Phe Ser Asp Arg Ala Asp Lys Ile Leu Ala 85
90 95 Lys Tyr Ala Ile Gly Lys Leu Val Gly Gly Ser Glu Phe Pro Thr
Tyr 100 105 110 Lys Pro Asp Thr Gly Phe Tyr Lys Glu Cys Cys Asp Arg
Val Asn Gln 115 120 125 Tyr Phe Lys Asp Asn Lys Leu Asp Pro Arg Ser
Pro Tyr Ser Gly Leu 130 135 140 Trp Arg Met Ile Leu Val Ala Ile Val
Gly Ala Val Ala Tyr Met Gly 145 150 155 160 Met Asn Gln Leu Leu Pro
Gly Asn Ile Tyr Ala His Tyr Ala Trp Gly 165 170 175 Ala Leu Phe Gly
Val Cys Gln Ala Leu Pro Leu Leu His Val Met His 180 185 190 Asp Ala
Ser His Ala Ala Ile Thr Ser Ser Pro Thr Gly Trp Arg Leu 195 200 205
Ile Gly Arg Leu Ala Met Asp Trp Val Ala Gly Ala Asn Met Val Ser 210
215 220 Trp Leu Asn Gln His Val Val Gly His His Ile Tyr Thr Asn Val
Ala 225 230 235 240 Gly Ala Asp Pro Asp Leu Pro Val Asp Phe Lys Ser
Asp Val Arg Arg 245 250 255 Ile Val Tyr Arg Gln Val Leu Leu Pro Ile
Tyr Lys Tyr Gln His Leu 260 265 270 Tyr Leu Pro Pro Leu Tyr Gly Val
Leu Gly Leu Lys Phe Arg Val Gln 275 280 285 Asp Val Phe Glu Thr Phe
Val Thr Leu Thr Asn Gly Pro Leu Arg Val 290 295 300 Asn Pro Leu Ser
Val Gly Asp Trp Ala Glu Met Ile Leu Ser Lys Ala 305 310 315 320 Phe
Trp Val Phe Tyr Arg Ile Tyr Leu Pro Leu Ala Val Leu Gln Val 325 330
335 Asp Pro Ala Arg Phe Trp Gly Val Phe Phe Leu Ala Glu Phe Ser Thr
340 345 350 Gly Trp Tyr Leu Ala Phe Asn Phe Gln Val Ser His Val Ser
Thr Ala 355 360 365 Cys Glu Tyr Pro Gly Gly Asp Glu Glu Val Thr Ser
Ile Asp Asp Glu 370 375 380 Trp Ala Ile Ser Gln Val Lys Ser Ser Val
Asp Tyr Gly His Gly Ser 385 390 395 400 Phe Ile Thr Thr Phe Leu Thr
Gly Ala Leu Asn Tyr Gln Val Thr His 405 410 415 His Leu Phe Pro Gly
Val Ser Gln Tyr His Tyr Pro Ala Ile Ala Pro 420 425 430 Leu Ile Leu
Asp Val Cys His Lys Tyr Lys Val Lys Tyr Asn Val Leu 435 440 445 Pro
Asp Phe Thr Ala Ala Met Ala Gly His Phe Asp His Leu Val Ile 450 455
460 Met Gly Lys Met Gly Lys Arg Val Thr Ile His Met Gly 465 470 475
<210> SEQ ID NO 14 <211> LENGTH: 469 <212> TYPE:
PRT <213> ORGANISM: Phaeodactylum tricornutum <220>
FEATURE: GenBank Accession No. AAL92562 <221> NAME/KEY:
MISC_FEATURE <223> OTHER INFORMATION: delta-5 desaturase
<400> SEQUENCE: 14 Met Ala Pro Asp Ala Asp Lys Leu Arg Gln
Arg Gln Thr Thr Ala Val 1 5 10 15 Ala Lys His Asn Ala Ala Thr Ile
Ser Thr Gln Glu Arg Leu Cys Ser 20 25 30 Leu Ser Ser Leu Lys Gly
Glu Glu Val Cys Ile Asp Gly Ile Ile Tyr 35 40 45 Asp Leu Gln Ser
Phe Asp His Pro Gly Gly Glu Thr Ile Lys Met Phe 50 55 60 Gly Gly
Asn Asp Val Thr Val Gln Tyr Lys Met Ile His Pro Tyr His 65 70 75 80
Thr Glu Lys His Leu Glu Lys Met Lys Arg Val Gly Lys Val Thr Asp 85
90 95 Phe Val Cys Glu Tyr Lys Phe Asp Thr Glu Phe Glu Arg Glu Ile
Lys 100 105 110 Arg Glu Val Phe Lys Ile Val Arg Arg Gly Lys Asp Phe
Gly Thr Leu 115 120 125 Gly Trp Phe Phe Arg Ala Phe Cys Tyr Ile Ala
Ile Phe Phe Tyr Leu 130 135 140 Gln Tyr His Trp Val Thr Thr Gly Thr
Ser Trp Leu Leu Ala Val Ala 145 150 155 160 Tyr Gly Ile Ser Gln Ala
Met Ile Gly Met Asn Val Gln His Asp Ala 165 170 175 Asn His Gly Ala
Thr Ser Lys Arg Pro Trp Val Asn Asp Met Leu Gly 180 185 190 Leu Gly
Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Gln Glu Gln 195 200 205
His Trp Thr His His Ala Tyr Thr Asn His Ala Glu Met Asp Pro Asp 210
215 220 Ser Phe Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu
Asp 225 230 235 240 His Pro Ala Arg Thr Trp Leu His Arg Phe Gln Ala
Phe Phe Tyr Met 245 250 255 Pro Val Leu Ala Gly Tyr Trp Leu Ser Ala
Val Phe Asn Pro Gln Ile 260 265 270 Leu Asp Leu Gln Gln Arg Gly Ala
Leu Ser Val Gly Ile Arg Leu Asp 275 280 285 Asn Ala Phe Ile His Ser
Arg Arg Lys Tyr Ala Val Phe Trp Arg Ala 290 295 300 Val Tyr Ile Ala
Val Asn Val Ile Ala Pro Phe Tyr Thr Asn Ser Gly 305 310 315 320 Leu
Glu Trp Ser Trp Arg Val Phe Gly Asn Ile Met Leu Met Gly Val 325 330
335 Ala Glu Ser Leu Ala Leu Ala Val Leu Phe Ser Leu Ser His Asn Phe
340 345 350 Glu Ser Ala Asp Arg Asp Pro Thr Ala Pro Leu Lys Lys Thr
Gly Glu 355 360 365 Pro Val Asp Trp Phe Lys Thr Gln Val Glu Thr Ser
Cys Thr Tyr Gly 370 375 380 Gly Phe Leu Ser Gly Cys Phe Thr Gly Gly
Leu Asn Phe Gln Val Glu 385 390 395 400 His His Leu Phe Pro Arg Met
Ser Ser Ala Trp Tyr Pro Tyr Ile Ala 405 410 415 Pro Lys Val Arg Glu
Ile Cys Ala Lys His Gly Val His Tyr Ala Tyr 420 425 430 Tyr Pro Trp
Ile His Gln Asn Phe Leu Ser Thr Val Arg Tyr Met His 435 440 445 Ala
Ala Gly Thr Gly Ala Asn Trp Arg Gln Met Ala Arg Glu Asn Pro 450 455
460 Leu Thr Gly Arg Ala 465 <210> SEQ ID NO 15 <211>
LENGTH: 467 <212> TYPE: PRT <213> ORGANISM:
Dictyostelium discoideum <220> FEATURE: GenBank Accession No.
XP_640331 <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: delta-5 desaturase <400> SEQUENCE: 15 Met Met
Glu Thr Asn Asn Glu Asn Lys Glu Lys Leu Lys Leu Tyr Thr 1 5 10 15
Trp Asp Glu Val Ser Lys His Asn Gln Lys Asn Asp Leu Trp Ile Ile 20
25 30 Val Asp Gly Lys Val Tyr Asn Ile Thr Lys Trp Val Pro Leu His
Pro 35 40 45 Gly Gly Glu Asp Ile Leu Leu Leu Ser Ala Gly Arg Asp
Ala Thr Asn 50 55 60 Leu Phe Glu Ser Tyr His Pro Met Thr Asp Lys
His Tyr Ser Leu Ile 65 70 75 80 Lys Gln Tyr Glu Ile Gly Tyr Ile Ser
Ser Tyr Glu His Pro Lys Tyr 85 90 95 Val Glu Lys Ser Glu Phe Tyr
Ser Thr Leu Lys Gln Arg Val Arg Lys 100 105 110 His Phe Gln Thr Ser
Ser Gln Asp Pro Lys Val Ser Val Gly Val Phe 115 120 125 Thr Arg Met
Val Leu Ile Tyr Leu Phe Leu Phe Val Thr Tyr Tyr Leu 130 135 140 Ser
Gln Phe Ser Thr Asp Arg Phe Trp Leu Asn Cys Ile Phe Ala Val 145 150
155 160 Leu Tyr Gly Val Ala Asn Ser Leu Phe Gly Leu His Thr Met His
Asp 165 170 175 Ala Cys His Thr Ala Ile Thr His Asn Pro Met Thr Trp
Lys Ile Leu 180 185 190 Gly Ala Thr Phe Asp Leu Phe Ala Gly Ala Ser
Phe Tyr Ala Trp Cys 195 200 205 His Gln His Val Ile Gly His His Leu
Tyr Thr Asn Val Arg Asn Ala 210 215 220 Asp Pro Asp Leu Gly Gln Gly
Glu Ile Asp Phe Arg Val Val Thr Pro 225 230 235 240 Tyr Gln Ala Arg
Ser Trp Tyr His Lys Tyr Gln His Ile Tyr Ala Pro 245 250 255 Ile Leu
Tyr Gly Val Tyr Ala Leu Lys Tyr Arg Ile Gln Asp His Glu 260 265 270
Ile Phe Thr Lys Lys Ser Asn Gly Ala Ile Arg Tyr Ser Pro Ile Ser 275
280 285 Thr Ile Asp Thr Ala Ile Phe Ile Leu Gly Lys Leu Val Phe Ile
Ile 290 295 300 Ser Arg Phe Ile Leu Pro Leu Ile Tyr Asn His Ser Phe
Ser His Leu 305 310 315 320 Ile Cys Phe Phe Leu Ile Ser Glu Leu Val
Leu Gly Trp Tyr Leu Ala 325 330 335 Ile Ser Phe Gln Val Ser His Val
Val Glu Asp Leu Gln Phe Met Ala 340 345 350 Thr Pro Glu Ile Phe Asp
Gly Ala Asp His Pro Leu Pro Thr Thr Phe 355 360 365 Asn Gln Asp Trp
Ala Ile Leu Gln Val Lys Thr Thr Gln Asp Tyr Ala 370 375 380 Gln Asp
Ser Val Leu Ser Thr Phe Phe Ser Gly Gly Leu Asn Leu Gln 385 390 395
400 Val Ile His His Cys Phe Pro Thr Ile Ala Gln Asp Tyr Tyr Pro Gln
405 410 415 Ile Val Pro Ile Leu Lys Glu Val Cys Lys Glu Tyr Asn Val
Thr Tyr 420 425 430 His Tyr Lys Pro Thr Phe Thr Glu Ala Ile Lys Ser
His Ile Asn Tyr 435 440 445 Leu Tyr Lys Met Gly Asn Asp Pro Asp Tyr
Val Arg Lys Pro Val Asn 450 455 460 Lys Asn Asp 465 <210> SEQ
ID NO 16 <211> LENGTH: 421 <212> TYPE: PRT <213>
ORGANISM: Euglena gracilis <220> FEATURE: <221>
NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION: delta-8
desaturase <300> PUBLICATION INFORMATION: <302> TITLE:
DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY
ACIDS <310> PATENT DOCUMENT NUMBER: WO2006012325 <311>
PATENT FILING DATE: 2005-06-24 <312> PUBLICATION DATE:
2006-02-02 <313> RELEVANT RESIDUES IN SEQ ID NO: (1)..(421)
<300> PUBLICATION INFORMATION: <302> TITLE: DELTA-8
DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310> PATENT DOCUMENT NUMBER: WO2006012326 <311> PATENT
FILING DATE: 2005-06-24 <312> PUBLICATION DATE: 2006-02-02
<313> RELEVANT RESIDUES IN SEQ ID NO: (1)..(421) <400>
SEQUENCE: 16 Met Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp
Gly Thr Thr 1 5 10 15 Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro
Gly Gly Ala Glu Ile 20 25 30 Ile Glu Asn Tyr Gln Gly Arg Asp Ala
Thr Asp Ala Phe Met Val Met 35 40 45 His Ser Gln Glu Ala Phe Asp
Lys Leu Lys Arg Met Pro Lys Ile Asn 50 55 60 Pro Ser Ser Glu Leu
Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu 65 70 75 80 Asp Phe Arg
Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp 85 90 95 Ala
Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly Leu 100 105
110 Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe Ile
115 120 125 Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp
Leu Ser 130 135 140 His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg
Asn Trp Asn Asn 145 150 155 160 Leu Val Gly Leu Val Phe Gly Asn Gly
Leu Gln Gly Phe Ser Val Thr 165 170 175 Trp Trp Lys Asp Arg His Asn
Ala His His Ser Ala Thr Asn Val Gln 180 185 190 Gly His Asp Pro Asp
Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu 195 200 205 Asp Asp Val
Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe 210 215 220 Gln
Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp 225 230
235 240 Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp
Asn 245 250 255 Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly
Leu Ala Leu 260 265 270 His Trp Thr Leu Lys Thr Leu Phe His Leu Phe
Phe Met Pro Ser Ile 275 280 285 Leu Thr Ser Leu Leu Val Phe Phe Val
Ser Glu Leu Val Gly Gly Phe 290 295 300 Gly Ile Ala Ile Val Val Phe
Met Asn His Tyr Pro Leu Glu Lys Ile 305 310 315 320 Gly Asp Ser Val
Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His 325 330 335 Glu Thr
Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly 340 345 350
Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg 355
360 365 His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln
Lys 370 375 380 His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly
Leu Val Ile 385 390 395 400 Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg
Met Ala Glu Lys Gln Pro 405 410 415 Ala Gly Lys Ala Leu 420
<210> SEQ ID NO 17 <211> LENGTH: 1269 <212> TYPE:
DNA <213> ORGANISM: Pavlova lutheri <220> FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
delta-8 desaturase <400> SEQUENCE: 17 atgggcaagg gtggagacgg
cggcgcgcag gcggtgagcg ggaccgacgc gtctctcgct 60 gaggtgagct
ccgtcgatag caagagcgtg cacgtcgtgc tctacggcaa gcgcgtggat 120
gtcacaaagt tccagaaggc acacccgggc gggagcaagg tgttccgcat cttccaggag
180 cgcgacgcga cggagcagtt cgagtcttac cactcgccca aggccatcaa
gatgatggag 240 ggcatgctca agaagtcgga ggatgcgccc gcttccgtgc
ccctgccctc gcggtccacc 300 atgggcacgg agttcaagga gatgattgag
cgccacaaga gggctggtct ctacgaccct 360 tgcccgttgg acgagctgtt
caagctcacc atcgtccttg cgcccatctt cgtgggcgcc 420 tatctcgtgc
ggagcggcgt ctcgcccctc gcgggcgcgc tctccatggg ctttggcttc 480
tacctcgacg gctggcttgc tcacgactac ctgcatcacg cagtcttcaa gggctcggtc
540 aacacgctcg tcaaggcgaa caacgccatg ggatacgccc tcggcttcct
ccagggctac 600 gacgtggcct ggtggcgcgc gcgccataac acgcaccacg
tgtgcaccaa cgaggatggt 660 tcggacccgg acatcaagac ggcgcccctg
ctcatctacg tgcgagagaa cccgtccatt 720 gccaagcggc tcaacttctt
ccagcgctgg cagcagtact actatgtgcc gaccatggcc 780 atcctcgacc
tctactggcg cctggagtcc atcgcgtacg tggctgtgcg cctgcctaag 840
atgtggatgc aggccgccgc tcttgccgct cactacgcgc tcctgtgctg ggtcttcgca
900 gcgcatctca acctcatccc tctcatgatg gttgcacgcg gcttcgcgac
gggcatcgtt 960 gtctttgcaa cccactatgg tgaggacatc ctcgaccgcg
agcacgtcga gggcatgacg 1020 ctcgtcgagc agaccgccaa gacctcccgt
aacatcacgg gcggctggct agtgaacgtg 1080 ctcacgggct tcatctccct
gcagaccgag catcacctct tccccatgat gcccaccggc 1140 aacctaatga
ctatccagcc cgaggtacgc gacttcttca agaagcatgg cctcgagtac 1200
cgcgagggca acctcttcca gtgcgtgcac cagaacatca aggctctcgc cttcgagcac
1260 ctcctccac 1269 <210> SEQ ID NO 18 <211> LENGTH:
423 <212> TYPE: PRT <213> ORGANISM: Pavlova lutheri
<400> SEQUENCE: 18 Met Gly Lys Gly Gly Asp Gly Gly Ala Gln
Ala Val Ser Gly Thr Asp 1 5 10 15 Ala Ser Leu Ala Glu Val Ser Ser
Val Asp Ser Lys Ser Val His Val 20 25 30 Val Leu Tyr Gly Lys Arg
Val Asp Val Thr Lys Phe Gln Lys Ala His 35 40 45 Pro Gly Gly Ser
Lys Val Phe Arg Ile Phe Gln Glu Arg Asp Ala Thr 50 55 60 Glu Gln
Phe Glu Ser Tyr His Ser Pro Lys Ala Ile Lys Met Met Glu 65 70 75 80
Gly Met Leu Lys Lys Ser Glu Asp Ala Pro Ala Ser Val Pro Leu Pro 85
90 95 Ser Arg Ser Thr Met Gly Thr Glu Phe Lys Glu Met Ile Glu Arg
His 100 105 110 Lys Arg Ala Gly Leu Tyr Asp Pro Cys Pro Leu Asp Glu
Leu Phe Lys 115 120 125 Leu Thr Ile Val Leu Ala Pro Ile Phe Val Gly
Ala Tyr Leu Val Arg 130 135 140 Ser Gly Val Ser Pro Leu Ala Gly Ala
Leu Ser Met Gly Phe Gly Phe 145 150 155 160 Tyr Leu Asp Gly Trp Leu
Ala His Asp Tyr Leu His His Ala Val Phe 165 170 175 Lys Gly Ser Val
Asn Thr Leu Val Lys Ala Asn Asn Ala Met Gly Tyr 180 185 190 Ala Leu
Gly Phe Leu Gln Gly Tyr Asp Val Ala Trp Trp Arg Ala Arg 195 200 205
His Asn Thr His His Val Cys Thr Asn Glu Asp Gly Ser Asp Pro Asp 210
215 220 Ile Lys Thr Ala Pro Leu Leu Ile Tyr Val Arg Glu Asn Pro Ser
Ile 225 230 235 240 Ala Lys Arg Leu Asn Phe Phe Gln Arg Trp Gln Gln
Tyr Tyr Tyr Val 245 250 255 Pro Thr Met Ala Ile Leu Asp Leu Tyr Trp
Arg Leu Glu Ser Ile Ala 260 265 270 Tyr Val Ala Val Arg Leu Pro Lys
Met Trp Met Gln Ala Ala Ala Leu 275 280 285 Ala Ala His Tyr Ala Leu
Leu Cys Trp Val Phe Ala Ala His Leu Asn 290 295 300 Leu Ile Pro Leu
Met Met Val Ala Arg Gly Phe Ala Thr Gly Ile Val 305 310 315 320 Val
Phe Ala Thr His Tyr Gly Glu Asp Ile Leu Asp Arg Glu His Val 325 330
335 Glu Gly Met Thr Leu Val Glu Gln Thr Ala Lys Thr Ser Arg Asn Ile
340 345 350 Thr Gly Gly Trp Leu Val Asn Val Leu Thr Gly Phe Ile Ser
Leu Gln 355 360 365 Thr Glu His His Leu Phe Pro Met Met Pro Thr Gly
Asn Leu Met Thr 370 375 380 Ile Gln Pro Glu Val Arg Asp Phe Phe Lys
Lys His Gly Leu Glu Tyr 385 390 395 400 Arg Glu Gly Asn Leu Phe Gln
Cys Val His Gln Asn Ile Lys Ala Leu 405 410 415 Ala Phe Glu His Leu
Leu His 420 <210> SEQ ID NO 19 <211> LENGTH: 7
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Conserved
region #1 within delta-5 and delta-8 desaturases <220>
FEATURE: <221> NAME/KEY: MISC_FEATURE <222> LOCATION:
(4)..(4) <223> OTHER INFORMATION: X = Ile or Val <400>
SEQUENCE: 19 Gly His His Xaa Tyr Thr Asn 1 5 <210> SEQ ID NO
20 <211> LENGTH: 7 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Conserved region #2 within delta-5 and delta-8
desaturases <220> FEATURE: <221> NAME/KEY: MISC_FEATURE
<222> LOCATION: (2)..(2) <223> OTHER INFORMATION: X = Y
or F <220> FEATURE: <221> NAME/KEY: MISC_FEATURE
<222> LOCATION: (4)..(4) <223> OTHER INFORMATION: X =
Val or Ile <400> SEQUENCE: 20 Asn Xaa Gln Xaa Glu His His 1 5
<210> SEQ ID NO 21 <211> LENGTH: 476 <212> TYPE:
PRT <213> ORGANISM: Thalassiosira pseudonana <220>
FEATURE: GenBank Accession No. AAX14502 <221> NAME/KEY:
MISC_FEATURE <223> OTHER INFORMATION: delta-8 desaturase
<400> SEQUENCE: 21 Met Pro Pro Asn Ala Asp Ile Ser Arg Ile
Arg Asn Arg Ile Pro Thr 1 5 10 15 Lys Thr Gly Thr Val Ala Ser Ala
Asp Asn Asn Asp Pro Ala Thr Gln 20 25 30 Ser Val Arg Thr Leu Lys
Ser Leu Lys Gly Asn Glu Val Val Ile Asn 35 40 45 Gly Thr Ile Tyr
Asp Ile Ala Asp Phe Val His Pro Gly Gly Glu Val 50 55 60 Val Lys
Phe Phe Gly Gly Asn Asp Val Thr Ile Gln Tyr Asn Met Ile 65 70 75 80
His Pro Tyr His Thr Gly Lys His Leu Glu Lys Met Lys Ala Val Gly 85
90 95 Lys Val Val Asp Trp Gln Ser Asp Tyr Lys Phe Asp Thr Pro Phe
Glu 100 105 110 Arg Glu Ile Lys Ser Glu Val Phe Lys Ile Val Arg Arg
Gly Arg Glu 115 120 125 Phe Gly Thr Thr Gly Tyr Phe Leu Arg Ala Phe
Phe Tyr Ile Ala Leu 130 135 140 Phe Phe Thr Met Gln Tyr Thr Phe Ala
Thr Cys Thr Thr Phe Thr Thr 145 150 155 160 Tyr Asp His Trp Tyr Gln
Ser Gly Val Phe Ile Ala Ile Val Phe Gly 165 170 175 Ile Ser Gln Ala
Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn His 180 185 190 Gly Ala
Ala Ser Lys Arg Pro Trp Val Asn Asp Leu Leu Gly Phe Gly 195 200 205
Thr Asp Leu Ile Gly Ser Asn Lys Trp Asn Trp Met Ala Gln His Trp 210
215 220 Thr His His Ala Tyr Thr Asn His Ser Glu Lys Asp Pro Asp Ser
Phe 225 230 235 240 Ser Ser Glu Pro Met Phe Ala Phe Asn Asp Tyr Pro
Ile Gly His Pro 245 250 255 Lys Arg Lys Trp Trp His Arg Phe Gln Gly
Gly Tyr Phe Leu Phe Met 260 265 270 Leu Gly Leu Tyr Trp Leu Pro Thr
Val Phe Asn Pro Gln Phe Ile Asp 275 280 285 Leu Arg Gln Arg Gly Ala
Gln Tyr Val Gly Ile Gln Met Glu Asn Asp 290 295 300 Phe Ile Val Lys
Arg Arg Lys Tyr Ala Val Ala Leu Arg Met Met Tyr 305 310 315 320 Ile
Tyr Leu Asn Ile Val Ser Pro Phe Met Asn Asn Gly Leu Ser Trp 325 330
335 Ser Thr Phe Gly Ile Ile Met Leu Met Gly Ile Ser Glu Ser Leu Thr
340 345 350 Leu Ser Val Leu Phe Ser Leu Ser His Asn Phe Ile Asn Ser
Asp Arg 355 360 365 Asp Pro Thr Ala Asp Phe Lys Lys Thr Gly Glu Gln
Val Cys Trp Phe 370 375 380 Lys Ser Gln Val Glu Thr Ser Ser Thr Tyr
Gly Gly Phe Ile Ser Gly 385 390 395 400 Cys Leu Thr Gly Gly Leu Asn
Phe Gln Val Glu His His Leu Phe Pro 405 410 415 Arg Met Ser Ser Ala
Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg Glu 420 425 430 Val Cys Lys
Lys His Gly Met Ser Tyr Ala Tyr Tyr Pro Trp Ile Gly 435 440 445 Gln
Asn Leu Val Ser Thr Phe Lys Tyr Met His Arg Ala Gly Ser Gly 450 455
460 Ala Asn Trp Glu Leu Lys Pro Leu Ser Gly Ser Ala 465 470 475
<210> SEQ ID NO 22 <211> LENGTH: 8165 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pZUF17 <400> SEQUENCE:
22 gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
ctaactcaca 60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc
ggtttgcgta ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 300
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
360 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg 420 acaggactat aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
720 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc 780 tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct 960 acggggtctg
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1020
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1080 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc 1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact 1200 acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1380
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1440 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
aaggcgagtt 1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc
atggttatgg cagcactgca taattctctt 1620 actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740
gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
1800 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac 1860 tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt 1980 tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa 2040 tgtatttaga
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2160 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc 2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc
tccctttagg gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa
cttgattagg gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt
tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 2460
taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2520 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
aggctgcgca 2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt
acgccagctg gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa
cgccagggtt ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg
taatacgact cactataggg cgaattgggt accgggcccc 2760 ccctcgaggt
cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct 2820
tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat
2880 taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg
tattatatat 2940 atacatcatg atgatactga cagtcatgtc ccattgctaa
atagacagac tccatctgcc 3000 gcctccaact gatgttctca atatttaagg
ggtcatctcg cattgtttaa taataaacag 3060 actccatcta ccgcctccaa
atgatgttct caaaatatat tgtatgaact tatttttatt 3120 acttagtatt
attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa 3180
tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat
3240 gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc
gaaatcaaca 3300 gcaacgaaaa aaatcccttg tacaacataa atagtcatcg
agaaatatca actatcaaag 3360 aacagctatt cacacgttac tattgagatt
attattggac gagaatcaca cactcaactg 3420 tctttctctc ttctagaaat
acaggtacaa gtatgtacta ttctcattgt tcatacttct 3480 agtcatttca
tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca 3540
aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc
3600 tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta
tatatttatt 3660 tcttgttata taatcctttt gtttattaca tgggctggat
acataaaggt attttgattt 3720 aattttttgc ttaaattcaa tcccccctcg
ttcagtgtca actgtaatgg taggaaatta 3780 ccatactttt gaagaagcaa
aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga 3840 cgttccgcag
aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg 3900
ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta
3960 ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt
tttttctaat 4020 gattcattac cgctatgtat acctacttgt acttgtagta
agccgggtta ttggcgttca 4080 attaatcata gacttatgaa tctgcacggt
gtgcgctgcg agttactttt agcttatgca 4140 tgctacttgg gtgtaatatt
gggatctgtt cggaaatcaa cggatgctca atcgatttcg 4200 acagtaatta
attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt 4260
agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc
4320 cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc
gatcagacag 4380 gtcgtctgac catcatacaa gctgaacaag cgctccatac
ttgcacgctc tctatataca 4440 cagttaaatt acatatccat agtctaacct
ctaacagtta atcttctggt aagcctccca 4500 gccagccttc tggtatcgct
tggcctcctc aataggatct cggttctggc cgtacagacc 4560 tcggccgaca
attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg 4620
ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc
4680 ctcagagtcg cccttaggtc ggttctgggc aatgaagcca accacaaact
cggggtcgga 4740 tcgggcaagc tcaatggtct gcttggagta ctcgccagtg
gccagagagc ccttgcaaga 4800 cagctcggcc agcatgagca gacctctggc
cagcttctcg ttgggagagg ggactaggaa 4860 ctccttgtac tgggagttct
cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt 4920 ttcctcggca
ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt 4980
ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc
5040 aatatctgcg aactttctgt cctcgaacag gaagaaaccg tgcttaagag
caagttcctt 5100 gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg
atgtcgatat gggttttgat 5160 catgcacaca taaggtccga ccttatcggc
aagctcaatg agctccttgg tggtggtaac 5220 atccagagaa gcacacaggt
tggttttctt ggctgccacg agcttgagca ctcgagcggc 5280 aaaggcggac
ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag 5340
gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa
5400 gtatatgtta tggtaatagt tacgagttag ttgaacttat agatagactg
gactatacgg 5460 ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg
gcgtcgcctt tgccgacaaa 5520 aatgtgatca tgatgaaagc cagcaatgac
gttgcagctg atattgttgt cggccaaccg 5580 cgccgaaaac gcagctgtca
gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat 5640 ccaagcacac
tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata 5700
ctcgtcgact caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc
5760 gggttggcgg cgtatttgtg tcccaaaaaa cagccccaat tgccccggag
aagacggcca 5820 ggccgcctag atgacaaatt caacaactca cagctgactt
tctgccattg ccactagggg 5880 ggggcctttt tatatggcca agccaagctc
tccacgtcgg ttgggctgca cccaacaata 5940 aatgggtagg gttgcaccaa
caaagggatg ggatgggggg tagaagatac gaggataacg 6000 gggctcaatg
gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga 6060
caccattgca tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca
6120 ccacagaggt tccgagcact ttaggttgca ccaaatgtcc caccaggtgc
aggcagaaaa 6180 cgctggaaca gcgtgtacag tttgtcttaa caaaaagtga
gggcgctgag gtcgagcagg 6240 gtggtgtgac ttgttatagc ctttagagct
gcgaaagcgc gtatggattt ggctcatcag 6300 gccagattga gggtctgtgg
acacatgtca tgttagtgta cttcaatcgc cccctggata 6360 tagccccgac
aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg 6420
tacccacacc ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca
6480 tcttacaagc ggggggcttg tctagggtat atataaacag tggctctccc
aatcggttgc 6540 cagtctcttt tttcctttct ttccccacag attcgaaatc
taaactacac atcacacaat 6600 gcctgttact gacgtcctta agcgaaagtc
cggtgtcatc gtcggcgacg atgtccgagc 6660 cgtgagtatc cacgacaaga
tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc 6720 gaaagtcgct
agcaacacac actctctaca caaactaacc cagctctcca tggctgagga 6780
taagaccaag gtcgagttcc ctaccctgac tgagctgaag cactctatcc ctaacgcttg
6840 ctttgagtcc aacctcggac tctcgctcta ctacactgcc cgagcgatct
tcaacgcatc 6900 tgcctctgct gctctgctct acgctgcccg atctactccc
ttcattgccg ataacgttct 6960 gctccacgct ctggtttgcg ccacctacat
ctacgtgcag ggtgtcatct tctggggttt 7020 ctttaccgtc ggtcacgact
gtggtcactc tgccttctcc cgataccact ccgtcaactt 7080 catcattggc
tgcatcatgc actctgccat tctgactccc ttcgagtcct ggcgagtgac 7140
ccaccgacac catcacaaga acactggcaa cattgataag gacgagatct tctaccctca
7200 tcggtccgtc aaggacctcc aggacgtgcg acaatgggtc tacaccctcg
gaggtgcttg 7260 gtttgtctac ctgaaggtcg gatatgctcc tcgaaccatg
tcccactttg acccctggga 7320 ccctctcctg cttcgacgag cctccgctgt
catcgtgtcc ctcggagtct gggctgcctt 7380 cttcgctgcc tacgcctacc
tcacatactc gctcggcttt gccgtcatgg gcctctacta 7440 ctatgctcct
ctctttgtct ttgcttcgtt cctcgtcatt actaccttct tgcatcacaa 7500
cgacgaagct actccctggt acggtgactc ggagtggacc tacgtcaagg gcaacctgag
7560 ctccgtcgac cgatcgtacg gagctttcgt ggacaacctg tctcaccaca
ttggcaccca 7620 ccaggtccat cacttgttcc ctatcattcc ccactacaag
ctcaacgaag ccaccaagca 7680 ctttgctgcc gcttaccctc acctcgtgag
acgtaacgac gagcccatca ttactgcctt 7740 cttcaagacc gctcacctct
ttgtcaacta cggagctgtg cccgagactg ctcagatttt 7800 caccctcaaa
gagtctgccg ctgcagccaa ggccaagagc gactaagcgg ccgcaagtgt 7860
ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt
7920 caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg
atatttatgt 7980 ttgacacttg agaatgtacg atacaagcac tgtccaagta
caatactaaa catactgtac 8040 atactcatac tcgtacccgg gcaacggttt
cacttgagtg cagtggctag tgctcttact 8100 cgtacagtgt gcaatactgc
gtatcatagt ctttgatgta tatcgtattc attcatgtta 8160 gttgc 8165
<210> SEQ ID NO 23 <211> LENGTH: 8438 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pDMW367 <400>
SEQUENCE: 23 catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg
ttgcgattga 60 tggcatcctc tacgaccttg aagggcttgc caaagttcat
ccaggaggag atttgattct 120 cgcttctggt gcctctgatg cctcccctct
cttttattca atgcatccat acgtcaaacc 180 ggagaattcc aaattgcttc
aacagttcgt ccgagggaag catgaccgca cctcgaagga 240 cattgtctac
acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt 300
gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat
360 cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc
tgtggggcct 420 gttgactgga ttcatgcaca tgcagatcgg cttatccatc
cagcatgatg cgtcccacgg 480 ggccatcagc aagaagcctt gggtcaacgc
cctcttcgcc tacggcattg acgtcatcgg 540 atcgtcccgg tggatttggc
tgcagtcgca catcatgcgg caccacacct acaccaacca 600 gcacggcctc
gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc 660
cgccgcaaac accgcccgaa agtggttcca ccgcttccaa gcttggtaca tgtaccttgt
720 gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc
ggatgcagca 780 caatgacacc atcccagagt ctgtcacggc catgcgggaa
aatggctttc tgcggcgcta 840 ccgcacactt gcattcgtga tgcgagcttt
cttcatcttc cggaccgcat tcttgccctg 900 gtacctcact gggacctcat
tgctgatcac cattcctctg gtgcccaccg caactggtgc 960 cttcttgacg
ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga 1020
caagaactgc aaggttaaga gatctgagaa ggacgttgag gctgaccaaa ttgactggta
1080 tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt
tcttcactgg 1140 cggtctcaat ttccagatcg agcaccacct ctttccccgg
atgtcgtctt ggcactaccc 1200 cttcgtccag caggcggtcc gggagtgttg
cgaacgccat ggagtgcgat atgttttcta 1260 ccctaccatc gtcggcaaca
tcatctccac cctgaagtac atgcataagg tgggtgtcgt 1320 ccactgcgtg
aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag 1380
tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata
1440 gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac
acttgagaat 1500 gtacgataca agcactgtcc aagtacaata ctaaacatac
tgtacatact catactcgta 1560 cccgggcaac ggtttcactt gagtgcagtg
gctagtgctc ttactcgtac agtgtgcaat 1620 actgcgtatc atagtctttg
atgtatatcg tattcattca tgttagttgc gtacgagccg 1680 gaagcataaa
gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 1740
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
1800 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
tcgctcactg 1860 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
agctcactca aaggcggtaa 1920 tacggttatc cacagaatca ggggataacg
caggaaagaa catgtgagca aaaggccagc 1980 aaaaggccag gaaccgtaaa
aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2040 ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2100
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
2160 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
tctcatagct 2220 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
caagctgggc tgtgtgcacg 2280 aaccccccgt tcagcccgac cgctgcgcct
tatccggtaa ctatcgtctt gagtccaacc 2340 cggtaagaca cgacttatcg
ccactggcag cagccactgg taacaggatt agcagagcga 2400 ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2460
ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2520 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
tgcaagcagc 2580 agattacgcg cagaaaaaaa ggatctcaag aagatccttt
gatcttttct acggggtctg 2640 acgctcagtg gaacgaaaac tcacgttaag
ggattttggt catgagatta tcaaaaagga 2700 tcttcaccta gatcctttta
aattaaaaat gaagttttaa atcaatctaa agtatatatg 2760 agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 2820
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
2880 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
tcaccggctc 2940 cagatttatc agcaataaac cagccagccg gaagggccga
gcgcagaagt ggtcctgcaa 3000 ctttatccgc ctccatccag tctattaatt
gttgccggga agctagagta agtagttcgc 3060 cagttaatag tttgcgcaac
gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3120 cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3180
ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3240 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
actgtcatgc 3300 catccgtaag atgcttttct gtgactggtg agtactcaac
caagtcattc tgagaatagt 3360 gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc gcgccacata 3420 gcagaacttt aaaagtgctc
atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3480 tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3540
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3600 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
tttcaatatt 3660 attgaagcat ttatcagggt tattgtctca tgagcggata
catatttgaa tgtatttaga 3720 aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct gacgcgccct 3780 gtagcggcgc attaagcgcg
gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3840 ccagcgccct
agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3900
gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3960 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
ccatcgccct 4020 gatagacggt ttttcgccct ttgacgttgg agtccacgtt
ctttaatagt ggactcttgt 4080 tccaaactgg aacaacactc aaccctatct
cggtctattc ttttgattta taagggattt 4140 tgccgatttc ggcctattgg
ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4200 ttaacaaaat
attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga 4260
agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
4320 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
aaacgacggc 4380 cagtgaattg taatacgact cactataggg cgaattgggt
accgggcccc ccctcgaggt 4440 cgatggtgtc gataagcttg atatcgaatt
catgtcacac aaaccgatct tcgcctcaag 4500 gaaacctaat tctacatccg
agagactgcc gagatccagt ctacactgat taattttcgg 4560 gccaataatt
taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg 4620
atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact
4680 gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag
actccatcta 4740 ccgcctccaa atgatgttct caaaatatat tgtatgaact
tatttttatt acttagtatt 4800 attagacaac ttacttgctt tatgaaaaac
acttcctatt taggaaacaa tttataatgg 4860 cagttcgttc atttaacaat
ttatgtagaa taaatgttat aaatgcgtat gggaaatctt 4920 aaatatggat
agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa 4980
aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt
5040 cacacgttac tattgagatt attattggac gagaatcaca cactcaactg
tctttctctc 5100 ttctagaaat acaggtacaa gtatgtacta ttctcattgt
tcatacttct agtcatttca 5160 tcccacatat tccttggatt tctctccaat
gaatgacatt ctatcttgca aattcaacaa 5220 ttataataag atataccaaa
gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc 5280 ttctcgtatt
tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata 5340
taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc
5400 ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta
ccatactttt 5460 gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt
ccaggttaga cgttccgcag 5520 aatctagaat gcggtatgcg gtacattgtt
cttcgaacgt aaaagttgcg ctccctgaga 5580 tattgtacat ttttgctttt
acaagtacaa gtacatcgta caactatgta ctactgttga 5640 tgcatccaca
acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac 5700
cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata
5760 gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca
tgctacttgg 5820 gtgtaatatt gggatctgtt cggaaatcaa cggatgctca
atcgatttcg acagtaatta 5880 attaagtcat acacaagtca gctttcttcg
agcctcatat aagtataagt agttcaacgt 5940 attagcactg tacccagcat
ctccgtatcg agaaacacaa caacatgccc cattggacag 6000 atcatgcgga
tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac 6060
catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt
6120 acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca
gccagccttc 6180 tggtatcgct tggcctcctc aataggatct cggttctggc
cgtacagacc tcggccgaca 6240 attatgatat ccgttccggt agacatgaca
tcctcaacag ttcggtactg ctgtccgaga 6300 gcgtctccct tgtcgtcaag
acccaccccg ggggtcagaa taagccagtc ctcagagtcg 6360 cccttaggtc
ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc 6420
tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc
6480 agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa
ctccttgtac 6540 tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt
cagagacagt ttcctcggca 6600 ccagctcgca ggccagcaat gattccggtt
ccgggtacac cgtgggcgtt ggtgatatcg 6660 gaccactcgg cgattcggtg
acaccggtac tggtgcttga cagtgttgcc aatatctgcg 6720 aactttctgt
cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc 6780
acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca
6840 taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac
atccagagaa 6900 gcacacaggt tggttttctt ggctgccacg agcttgagca
ctcgagcggc aaaggcggac 6960 ttgtggacgt tagctcgagc ttcgtaggag
ggcattttgg tggtgaagag gagactgaaa 7020 taaatttagt ctgcagaact
ttttatcgga accttatctg gggcagtgaa gtatatgtta 7080 tggtaatagt
tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc 7140
aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca
7200 tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg
cgccgaaaac 7260 gcagctgtca gacccacagc ctccaacgaa gaatgtatcg
tcaaagtgat ccaagcacac 7320 tcatagttgg agtcgtactc caaaggcggc
aatgacgagt cagacagata ctcgtcgact 7380 caggcgacga cggaattcct
gcagcccatc tgcagaattc aggagagacc gggttggcgg 7440 cgtatttgtg
tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag 7500
atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt
7560 tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata
aatgggtagg 7620 gttgcaccaa caaagggatg ggatgggggg tagaagatac
gaggataacg gggctcaatg 7680 gcacaaataa gaacgaatac tgccattaag
actcgtgatc cagcgactga caccattgca 7740 tcatctaagg gcctcaaaac
tacctcggaa ctgctgcgct gatctggaca ccacagaggt 7800 tccgagcact
ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca 7860
gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac
7920 ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag
gccagattga 7980 gggtctgtgg acacatgtca tgttagtgta cttcaatcgc
cccctggata tagccccgac 8040 aataggccgt ggcctcattt ttttgccttc
cgcacatttc cattgctcgg tacccacacc 8100 ttgcttctcc tgcacttgcc
aaccttaata ctggtttaca ttgaccaaca tcttacaagc 8160 ggggggcttg
tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt 8220
tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact
8280 gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc
cgtgagtatc 8340 cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat
gacacaatcc gaaagtcgct 8400 agcaacacac actctctaca caaactaacc
cagctctc 8438 <210> SEQ ID NO 24 <211> LENGTH: 12649
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid
pKUNF12T6E <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (2507)..(2507) <223> OTHER INFORMATION:
n is a, c, g, or t <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (2512)..(2515) <223> OTHER
INFORMATION: n is a, c, g, or t <400> SEQUENCE: 24 taaccctcac
taaagggaac aaaagctgga gctccaccgc ggacacaata tctggtcaaa 60
tttcagtttc gttacataaa tcgttatgtc aaaggagtgt gggaggttaa gagaattatc
120 accggcaaac tatctgttaa ttgctaggta cctctagacg tccacccggg
tcgcttggcg 180 gccgaagagg ccggaatctc gggccgcggt ggcggccgct
tagttggtct tggacttctt 240 gggcttcttc aggtaggact ggacaaagaa
gttgccgaac agagcgagca gggtgatcat 300 gtacacgccg agcagctgga
ccagagcctg agggtagtcg caggggaaga ggtagtcgta 360 cagggactgc
accagcatag ccatgaactg ggtcatctgc agagtggtga tgtagggctt 420
gatgggcttg acgaagccga agccctgaga ggaaaagaag tagtaggcgt acatgacggt
480 gtggacgaag gagttgagga tgacggagaa gtaggcgtcg ccaccaggag
cgtacttggc 540 aatagcccac cagatggcga agatggtggc atggtggtac
acgtgcagga aggagacctg 600 gttgaacttc ttgcacagga tcatgatagc
ggtgtccagg aactcgtagg ccttggagac 660 gtagaacacg tagacgattc
gggacatgcc ctgagcgtgg gactcgttgc ccttctccat 720 gtcgttgccg
aagaccttgt agccacccag gatagcctgt cggatggtct cgacgcacat 780
gtagagggac agtccgaaga ggaacaggtt gtggagcagc ttgatggtct tcagctcgaa
840 gggcttctcc atctgcttca tgatgggaat gccgaagagc agcatggcca
tgtagccgac 900 ctcgaaggcg agcatggtgg agacgtccat catgggcaga
ccgtcggtca gagcgtaggg 960 cttagctccg tccatccact ggtcgacacc
ggtctcgact cgtccgacca cgtcgtccca 1020 gacagaggag ttggccatgg
tgaatgattc ttatactcag aaggaaatgc ttaacgattt 1080 cgggtgtgag
ttgacaagga gagagagaaa agaagaggaa aggtaattcg gggacggtgg 1140
tcttttatac ccttggctaa agtcccaacc acaaagcaaa aaaattttca gtagtctatt
1200 ttgcgtccgg catgggttac ccggatggcc agacaaagaa actagtacaa
agtctgaaca 1260 agcgtagatt ccagactgca gtaccctacg cccttaacgg
caagtgtggg aaccggggga 1320 ggtttgatat gtggggtgaa gggggctctc
gccggggttg ggcccgctac tgggtcaatt 1380 tggggtcaat tggggcaatt
ggggctgttt tttgggacac aaatacgccg ccaacccggt 1440 ctctcctgaa
ttctgcatcg atcgaggaag aggacaagcg gctgcttctt aagtttgtga 1500
catcagtatc caaggcacca ttgcaaggat tcaaggcttt gaacccgtca tttgccattc
1560 gtaacgctgg tagacaggtt gatcggttcc ctacggcctc cacctgtgtc
aatcttctca 1620 agctgcctga ctatcaggac attgatcaac ttcggaagaa
acttttgtat gccattcgat 1680 cacatgctgg tttcgatttg tcttagagga
acgcatatac agtaatcata gagaataaac 1740 gatattcatt tattaaagta
gatagttgag gtagaagttg taaagagtga taaatagcgg 1800 ccgcgcctac
ttaagcaacg ggcttgataa cagcgggggg ggtgcccacg ttgttgcggt 1860
tgcggaagaa cagaacaccc ttaccagcac cctcggcacc agcgctgggc tcaacccact
1920 ggcacatacg cgcactgcgg tacatggcgc ggatgaagcc acgaggacca
tcctggacat 1980 cagcccggta gtgcttgccc atgatgggct taatggcctc
ggtggcctcg tccgcgttgt 2040 agaaggggat gctgctgacg tagtggtgga
ggacatgagt ctcgatgatg ccgtggagaa 2100 ggtggcggcc gatgaagccc
atctcacggt caatggtagc agcggcacca cggacgaagt 2160 tccactcgtc
gttggtgtag tggggaaggg tagggtcggt gtgctggagg aaggtgatgg 2220
caacgagcca gtggttaacc cagaggtagg gaacaaagta ccagatggcc atgttgtaga
2280 aaccgaactt ctgaacgagg aagtacagag cagtggccat cagaccgata
ccaatatcgc 2340 tgaggacgat gagcttagcg tcactgttct cgtacagagg
gctgcgggga tcgaagtggt 2400 taacaccacc gccgaggccg ttatgcttgc
ccttgccgcg accctcacgc tggcgctcgt 2460 ggtagttgtg gccggtaaca
ttggtgatga ggtagttggg ccagccnacg annnnctcag 2520 taagatgagc
gagctcgtgg gtcatctttc cgagacgagt agcctgctgc tcgcgggttc 2580
ggggaacgaa gaccatgtca cgctccatgt tgccagtggc cttgtggtgc tttcggtggg
2640 agatttgcca gctgaagtag gggacaagga gggaagagtg aagaacccag
ccagtaatgt 2700 cgttgatgat gcgagaatcg gagaaagcac cgtgaccgca
ctcatgggca ataacccaga 2760 gaccagtacc gaaaagaccc tgaagaacgg
tgtacacggc ccacagacca gcgcgggcgg 2820 gggtggaggg gatatattcg
ggggtcacaa agttgtacca gatgctgaaa gtggtagtca 2880 ggaggacaat
gtcgcggagg atataaccgt atcccttgag agcggagcgc ttgaagcagt 2940
gcttagggat ggcattgtag atgtccttga tggtaaagtc gggaacctcg aactggttgc
3000 cgtaggtgtc gagcatgaca ccatactcgg acttgggctt ggcgatatca
acctcggaca 3060 tggacgagag cgatgtggaa gaggccgagt ggcggggaga
gtctgaagga gagacggcgg 3120 cagactcaga atccgtcaca gtagttgagg
tgacggtgcg tctaagcgca gggttctgct 3180 tgggcagagc cgaagtggac
gccatggaga gctgggttag tttgtgtaga gagtgtgtgt 3240 tgctagcgac
tttcggattg tgtcattaca caaaacgcgt cgtctcgaca ctgatcttgt 3300
cgtggatact cacggctcgg acatcgtcgc cgacgatgac accggacttt cgcttaagga
3360 cgtcagtaac aggcattgtg tgatgtgtag tttagatttc gaatctgtgg
ggaaagaaag 3420 gaaaaaagag actggcaacc gattgggaga gccactgttt
atatataccc tagacaagcc 3480 ccccgcttgt aagatgttgg tcaatgtaaa
ccagtattaa ggttggcaag tgcaggagaa 3540 gcaaggtgtg ggtaccgagc
aatggaaatg tgcggaaggc aaaaaaatga ggccacggcc 3600 tattgtcggg
gctatatcca gggggcgatt gaagtacact aacatgacat gtgtccacag 3660
accctcaatc tggcctgatg agccaaatcc atacgcgctt tcgcagctct aaaggctata
3720 acaagtcaca ccaccctgct cgacctcagc gccctcactt tttgttaaga
caaactgtac 3780 acgctgttcc agcgttttct gcctgcacct ggtgggacat
ttggtgcaac ctaaagtgct 3840 cggaacctct gtggtgtcca gatcagcgca
gcagttccga ggtagttttg aggcccttag 3900 atgatgcaat ggtgtcagtc
gctggatcac gagtcttaat ggcagtattc gttcttattt 3960 gtgccattga
gccccgttat cctcgtatct tctacccccc atcccatccc tttgttggtg 4020
caaccctacc catttattgt tgggtgcagc ccaaccgacg tggagagctt ggcttggcca
4080 tataaaaagg ccccccccta gtggcaatgg cagaaagtca gctgtgagtt
gttgaatttg 4140 tcatctaggc ggcctggccg tcttctccgg ggcaattgtt
cctctatagt actgcgtaca 4200 ctgtttaaac agtgtacgca gatctgcgac
gacggaattc ctgcagccca tctgcagaat 4260 tcaggagaga ccgggttggc
ggcgtatttg tgtcccaaaa aacagcccca attgccccaa 4320 ttgaccccaa
attgacccag tagcgggccc aaccccggcg agagccccct tcaccccaca 4380
tatcaaacct cccccggttc ccacacttgc cgttaagggc gtagggtact gcagtctgga
4440 atctacgctt gttcagactt tgtactagtt tctttgtctg gccatccggg
taacccatgc 4500 cggacgcaaa atagactact gaaaattttt ttgctttgtg
gttgggactt tagccaaggg 4560 tataaaagac caccgtcccc gaattacctt
tcctcttctt ttctctctct ccttgtcaac 4620 tcacacccga aatcgttaag
catttccttc tgagtataag aatcattcac catggctgcc 4680 gctccctctg
tgcgaacctt tacccgagcc gaggttctga acgctgaggc tctgaacgag 4740
ggcaagaagg acgctgaggc tcccttcctg atgatcatcg acaacaaggt gtacgacgtc
4800 cgagagttcg tccctgacca tcctggaggc tccgtgattc tcacccacgt
tggcaaggac 4860 ggcaccgacg tctttgacac ctttcatccc gaggctgctt
gggagactct cgccaacttc 4920 tacgttggag acattgacga gtccgaccga
gacatcaaga acgatgactt tgccgctgag 4980 gtccgaaagc tgcgaaccct
gttccagtct ctcggctact acgactcctc taaggcctac 5040 tacgccttca
aggtctcctt caacctctgc atctggggac tgtccaccgt cattgtggcc 5100
aagtggggtc agacctccac cctcgccaac gtgctctctg ctgccctgct cggcctgttc
5160 tggcagcagt gcggatggct ggctcacgac tttctgcacc accaggtctt
ccaggaccga 5220 ttctggggtg atctcttcgg agccttcctg ggaggtgtct
gccagggctt ctcctcttcc 5280 tggtggaagg acaagcacaa cactcaccat
gccgctccca acgtgcatgg cgaggatcct 5340 gacattgaca cccaccctct
cctgacctgg tccgagcacg ctctggagat gttctccgac 5400 gtccccgatg
aggagctgac ccgaatgtgg tctcgattca tggtcctgaa ccagacctgg 5460
ttctacttcc ccattctctc cttcgctcga ctgtcttggt gcctccagtc cattctcttt
5520 gtgctgccca acggtcaggc tcacaagccc tccggagctc gagtgcccat
ctccctggtc 5580 gagcagctgt ccctcgccat gcactggacc tggtacctcg
ctaccatgtt cctgttcatc 5640 aaggatcctg tcaacatgct cgtgtacttc
ctggtgtctc aggctgtgtg cggaaacctg 5700 ctcgccatcg tgttctccct
caaccacaac ggtatgcctg tgatctccaa ggaggaggct 5760 gtcgacatgg
atttctttac caagcagatc atcactggtc gagatgtcca tcctggactg 5820
ttcgccaact ggttcaccgg tggcctgaac taccagatcg agcatcacct gttcccttcc
5880 atgcctcgac acaacttctc caagatccag cctgccgtcg agaccctgtg
caagaagtac 5940 aacgtccgat accacaccac tggtatgatc gagggaactg
ccgaggtctt ctcccgactg 6000 aacgaggtct ccaaggccac ctccaagatg
ggcaaggctc agtaagcggc cgcatgagaa 6060 gataaatata taaatacatt
gagatattaa atgcgctaga ttagagagcc tcatactgct 6120 cggagagaag
ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa 6180
gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg
6240 gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc
ttggcctcca 6300 tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa
cagaagcaga tgaagaatga 6360 acttgaagtg aaggaattta aattgccccg
gagaagacgg ccaggccgcc tagatgacaa 6420 attcaacaac tcacagctga
ctttctgcca ttgccactag gggggggcct ttttatatgg 6480 ccaagccaag
ctctccacgt cggttgggct gcacccaaca ataaatgggt agggttgcac 6540
caacaaaggg atgggatggg gggtagaaga tacgaggata acggggctca atggcacaaa
6600 taagaacgaa tactgccatt aagactcgtg atccagcgac tgacaccatt
gcatcatcta 6660 agggcctcaa aactacctcg gaactgctgc gctgatctgg
acaccacaga ggttccgagc 6720 actttaggtt gcaccaaatg tcccaccagg
tgcaggcaga aaacgctgga acagcgtgta 6780 cagtttgtct taacaaaaag
tgagggcgct gaggtcgagc agggtggtgt gacttgttat 6840 agcctttaga
gctgcgaaag cgcgtatgga tttggctcat caggccagat tgagggtctg 6900
tggacacatg tcatgttagt gtacttcaat cgccccctgg atatagcccc gacaataggc
6960 cgtggcctca tttttttgcc ttccgcacat ttccattgct cggtacccac
accttgcttc 7020 tcctgcactt gccaacctta atactggttt acattgacca
acatcttaca agcggggggc 7080 ttgtctaggg tatatataaa cagtggctct
cccaatcggt tgccagtctc ttttttcctt 7140 tctttcccca cagattcgaa
atctaaacta cacatcacac aatgcctgtt actgacgtcc 7200 ttaagcgaaa
gtccggtgtc atcgtcggcg acgatgtccg agccgtgagt atccacgaca 7260
agatcagtgt cgagacgacg cgttttgtgt aatgacacaa tccgaaagtc gctagcaaca
7320 cacactctct acacaaacta acccagctct ccatggagtc cattgctccc
ttcctgccct 7380 ccaagatgcc tcaggacctg ttcatggacc tcgccagcgc
tatcggtgtc cgagctgctc 7440 cctacgtcga tcccctggag gctgccctgg
ttgcccaggc cgagaagtac attcccacca 7500 ttgtccatca cactcgaggc
ttcctggttg ccgtggagtc tcccctggct cgagagctgc 7560 ctctgatgaa
ccccttccac gtgctcctga tcgtgctcgc ctacctggtc accgtgtttg 7620
tgggtatgca gatcatgaag aactttgaac gattcgaggt caagaccttc tccctcctgc
7680 acaacttctg tctggtctcc atctccgcct acatgtgcgg tggcatcctg
tacgaggctt 7740 atcaggccaa ctatggactg tttgagaacg ctgccgatca
caccttcaag ggtctcccta 7800 tggctaagat gatctggctc ttctacttct
ccaagatcat ggagtttgtc gacaccatga 7860 tcatggtcct caagaagaac
aaccgacaga tttcctttct gcacgtgtac caccactctt 7920 ccatcttcac
catctggtgg ctggtcacct tcgttgctcc caacggtgaa gcctacttct 7980
ctgctgccct gaactccttc atccacgtca tcatgtacgg ctactacttt ctgtctgccc
8040 tgggcttcaa gcaggtgtcg ttcatcaagt tctacatcac tcgatcccag
atgacccagt 8100 tctgcatgat gtctgtccag tcttcctggg acatgtacgc
catgaaggtc cttggccgac 8160 ctggataccc cttcttcatc accgctctgc
tctggttcta catgtggacc atgctcggtc 8220 tcttctacaa cttttaccga
aagaacgcca agctcgccaa gcaggccaag gctgacgctg 8280 ccaaggagaa
ggccagaaag ctccagtaag cggccgcaag tgtggatggg gaagtgagtg 8340
cccggttctg tgtgcacaat tggcaatcca agatggatgg attcaacaca gggatatagc
8400 gagctacgtg gtggtgcgag gatatagcaa cggatattta tgtttgacac
ttgagaatgt 8460 acgatacaag cactgtccaa gtacaatact aaacatactg
tacatactca tactcgtacc 8520 cgggcaacgg tttcacttga gtgcagtggc
tagtgctctt actcgtacag tgtgcaatac 8580 tgcgtatcat agtctttgat
gtatatcgta ttcattcatg ttagttgcgt acgaagtcgt 8640 caatgatgtc
gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct 8700
caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg
8760 ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct
cgagcttcgt 8820 aggagggcat tttggtggtg aagaggagac tgaaataaat
ttagtctgca gaacttttta 8880 tcggaacctt atctggggca gtgaagtata
tgttatggta atagttacga gttagttgaa 8940 cttatagata gactggacta
tacggctatc ggtccaaatt agaaagaacg tcaatggctc 9000 tctgggcgtc
gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc 9060
agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca
9120 acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg
tactccaaag 9180 gcggcaatga cgagtcagac agatactcgt cgaccttttc
cttgggaacc accaccgtca 9240 gcccttctga ctcacgtatt gtagccaccg
acacaggcaa cagtccgtgg atagcagaat 9300 atgtcttgtc ggtccatttc
tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt 9360 atgtgccttc
attgagaatc ggtgttgctg atttcaataa agtcttgaga tcagtttggc 9420
gcgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
9480 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt 9540 atcagctcac tcaaaggcgg taatacggtt atccacagaa
tcaggggata acgcaggaaa 9600 gaacatgtga gcaaaaggcc agcaaaaggc
caggaaccgt aaaaaggccg cgttgctggc 9660 gtttttccat aggctccgcc
cccctgacga gcatcacaaa aatcgacgct caagtcagag 9720 gtggcgaaac
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 9780
gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
9840 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg 9900 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg 9960 taactatcgt cttgagtcca acccggtaag
acacgactta tcgccactgg cagcagccac 10020 tggtaacagg attagcagag
cgaggtatgt aggcggtgct acagagttct tgaagtggtg 10080 gcctaactac
ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 10140
taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
10200 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc 10260 tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt aagggatttt 10320 ggtcatgaga ttatcaaaaa ggatcttcac
ctagatcctt ttaaattaaa aatgaagttt 10380 taaatcaatc taaagtatat
atgagtaaac ttggtctgac agttaccaat gcttaatcag 10440 tgaggcacct
atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 10500
cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
10560 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc 10620 cgagcgcaga agtggtcctg caactttatc cgcctccatc
cagtctatta attgttgccg 10680 ggaagctaga gtaagtagtt cgccagttaa
tagtttgcgc aacgttgttg ccattgctac 10740 aggcatcgtg gtgtcacgct
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 10800 atcaaggcga
gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 10860
tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
10920 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc 10980 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat 11040 acgggataat accgcgccac atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc 11100 ttcggggcga aaactctcaa
ggatcttacc gctgttgaga tccagttcga tgtaacccac 11160 tcgtgcaccc
aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 11220
aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
11280 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg 11340 atacatattt gaatgtattt agaaaaataa acaaataggg
gttccgcgca catttccccg 11400 aaaagtgcca cctgatgcgg tgtgaaatac
cgcacagatg cgtaaggaga aaataccgca 11460 tcaggaaatt gtaagcgtta
atattttgtt aaaattcgcg ttaaattttt gttaaatcag 11520 ctcatttttt
aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 11580
cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
11640 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
gtgaaccatc 11700 accctaatca agttttttgg ggtcgaggtg ccgtaaagca
ctaaatcgga accctaaagg 11760 gagcccccga tttagagctt gacggggaaa
gccggcgaac gtggcgagaa aggaagggaa 11820 gaaagcgaaa ggagcgggcg
ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 11880 caccacaccc
gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg 11940
cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
12000 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
gtcacgacgt 12060 tgtaaaacga cggccagtga attgtaatac gactcactat
agggcgaatt gggcccgacg 12120 tcgcatgcag tggtggtatt gtgactgggg
atgtagttga gaataagtca tacacaagtc 12180 agctttcttc gagcctcata
taagtataag tagttcaacg tattagcact gtacccagca 12240 tctccgtatc
gagaaacaca acaacatgcc ccattggaca gatcatgcgg atacacaggt 12300
tgtgcagtat catacatact cgatcagaca ggtcgtctga ccatcataca agctgaacaa
12360 gcgctccata cttgcacgct ctctatatac acagttaaat tacatatcca
tagtctaacc 12420 tctaacagtt aatcttctgg taagcctccc agccagcctt
ctggtatcgc ttggcctcct 12480 caataggatc tcggttctgg ccgtacagac
ctcggccgac aattatgata tccgttccgg 12540 tagacatgac atcctcaaca
gttcggtact gctgtccgag agcgtctccc ttgtcgtcaa 12600 gacccacccc
gggggtcaga ataagccagt cctcagagtc gcccttaat 12649 <210> SEQ ID
NO 25 <211> LENGTH: 819 <212> TYPE: DNA <213>
ORGANISM: Thraustochytrium aureum <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: synthetic
elongase (codon-optimized) for Yarrowia lipolytica <400>
SEQUENCE: 25 atggccaact cctctgtctg ggacgacgtg gtcggacgag tcgagaccgg
tgtcgaccag 60 tggatggacg gagctaagcc ctacgctctg accgacggtc
tgcccatgat ggacgtctcc 120 accatgctcg ccttcgaggt cggctacatg
gccatgctgc tcttcggcat tcccatcatg 180 aagcagatgg agaagccctt
cgagctgaag accatcaagc tgctccacaa cctgttcctc 240 ttcggactgt
ccctctacat gtgcgtcgag accatccgac aggctatcct gggtggctac 300
aaggtcttcg gcaacgacat ggagaagggc aacgagtccc acgctcaggg catgtcccga
360 atcgtctacg tgttctacgt ctccaaggcc tacgagttcc tggacaccgc
tatcatgatc 420 ctgtgcaaga agttcaacca ggtctccttc ctgcacgtgt
accaccatgc caccatcttc 480 gccatctggt gggctattgc caagtacgct
cctggtggcg acgcctactt ctccgtcatc 540 ctcaactcct tcgtccacac
cgtcatgtac gcctactact tcttttcctc tcagggcttc 600 ggcttcgtca
agcccatcaa gccctacatc accactctgc agatgaccca gttcatggct 660
atgctggtgc agtccctgta cgactacctc ttcccctgcg actaccctca ggctctggtc
720 cagctgctcg gcgtgtacat gatcaccctg ctcgctctgt tcggcaactt
ctttgtccag 780 tcctacctga agaagcccaa gaagtccaag accaactaa 819
<210> SEQ ID NO 26 <211> LENGTH: 272 <212> TYPE:
PRT <213> ORGANISM: Thraustochytrium aureum <400>
SEQUENCE: 26 Met Ala Asn Ser Ser Val Trp Asp Asp Val Val Gly Arg
Val Glu Thr 1 5 10 15 Gly Val Asp Gln Trp Met Asp Gly Ala Lys Pro
Tyr Ala Leu Thr Asp 20 25 30 Gly Leu Pro Met Met Asp Val Ser Thr
Met Leu Ala Phe Glu Val Gly 35 40 45 Tyr Met Ala Met Leu Leu Phe
Gly Ile Pro Ile Met Lys Gln Met Glu 50 55 60 Lys Pro Phe Glu Leu
Lys Thr Ile Lys Leu Leu His Asn Leu Phe Leu 65 70 75 80 Phe Gly Leu
Ser Leu Tyr Met Cys Val Glu Thr Ile Arg Gln Ala Ile 85 90 95 Leu
Gly Gly Tyr Lys Val Phe Gly Asn Asp Met Glu Lys Gly Asn Glu 100 105
110 Ser His Ala Gln Gly Met Ser Arg Ile Val Tyr Val Phe Tyr Val Ser
115 120 125 Lys Ala Tyr Glu Phe Leu Asp Thr Ala Ile Met Ile Leu Cys
Lys Lys 130 135 140 Phe Asn Gln Val Ser Phe Leu His Val Tyr His His
Ala Thr Ile Phe 145 150 155 160 Ala Ile Trp Trp Ala Ile Ala Lys Tyr
Ala Pro Gly Gly Asp Ala Tyr 165 170 175 Phe Ser Val Ile Leu Asn Ser
Phe Val His Thr Val Met Tyr Ala Tyr 180 185 190 Tyr Phe Phe Ser Ser
Gln Gly Phe Gly Phe Val Lys Pro Ile Lys Pro 195 200 205 Tyr Ile Thr
Thr Leu Gln Met Thr Gln Phe Met Ala Met Leu Val Gln 210 215 220 Ser
Leu Tyr Asp Tyr Leu Phe Pro Cys Asp Tyr Pro Gln Ala Leu Val 225 230
235 240 Gln Leu Leu Gly Val Tyr Met Ile Thr Leu Leu Ala Leu Phe Gly
Asn 245 250 255 Phe Phe Val Gln Ser Tyr Leu Lys Lys Pro Lys Lys Ser
Lys Thr Asn 260 265 270 <210> SEQ ID NO 27 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-1A <400> SEQUENCE: 27 gghcaycayr tbtayacaaa 20 <210>
SEQ ID NO 28 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-1B <400> SEQUENCE: 28
gghcaycayr tbtayaccaa 20 <210> SEQ ID NO 29 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-1C <400> SEQUENCE: 29 gghcaycayr tbtayacgaa 20 <210>
SEQ ID NO 30 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-1D <400> SEQUENCE: 30
gghcaycayr tbtayactaa 20 <210> SEQ ID NO 31 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-5AR <400> SEQUENCE: 31 tgrtgvacaa yytgrwartt 20 <210>
SEQ ID NO 32 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-5BR <400> SEQUENCE:
32 tgrtgvacta yytgrwartt 20 <210> SEQ ID NO 33 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-5CR <400> SEQUENCE: 33 tgrtgvacca yytgrwartt 20 <210>
SEQ ID NO 34 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-5DR <400> SEQUENCE:
34 tgrtgvacga yytgrwartt 20 <210> SEQ ID NO 35 <211>
LENGTH: 27 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
ODMW480 <400> SEQUENCE: 35 ccgataccag tcaatttggt cagcctc 27
<210> SEQ ID NO 36 <211> LENGTH: 23 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: CDSIII 5' primer <400>
SEQUENCE: 36 aagcagtggt atcaacgcag agt 23 <210> SEQ ID NO 37
<211> LENGTH: 28 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer ODMW479 <400> SEQUENCE: 37 cttaaccttg
cagttcttgt cggggatc 28 <210> SEQ ID NO 38 <211> LENGTH:
22 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer DNR CDS
5' <400> SEQUENCE: 38 caacgcagag tggccattac gg 22 <210>
SEQ ID NO 39 <211> LENGTH: 27 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer YL791 <400> SEQUENCE:
39 cacctcgcgc attgtccgct taacgtc 27 <210> SEQ ID NO 40
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer YL792 <400> SEQUENCE: 40 tgcgaaggga
gaatcatacg tgtagac 27 <210> SEQ ID NO 41 <211> LENGTH:
26 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer ODMW469
<400> SEQUENCE: 41 cttcatcttc cggaccgcat tcttgc 26
<210> SEQ ID NO 42 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AUAP <400> SEQUENCE: 42
ggccacgcgt cgactagtac 20 <210> SEQ ID NO 43 <211>
LENGTH: 28 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
ODMW470 <400> SEQUENCE: 43 cctcactggg acctcattgc tgatcacc 28
<210> SEQ ID NO 44 <211> LENGTH: 32 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer YL794 <400> SEQUENCE:
44 tttccatggc tctcagtctt accacagaac ag 32 <210> SEQ ID NO 45
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer YL797 <400> SEQUENCE: 45 gtacatgtac
caagcttgga agcggtg 27 <210> SEQ ID NO 46 <211> LENGTH:
27 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer YL796
<400> SEQUENCE: 46 caccgcttcc aagcttggta catgtac 27
<210> SEQ ID NO 47 <211> LENGTH: 39 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer YL795 <400> SEQUENCE:
47 tttgcggccg cttaggaatc ctgtgcgtcc ttcacgcag 39 <210> SEQ ID
NO 48 <211> LENGTH: 4070 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pEgD5S <400> SEQUENCE: 48
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga
gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat
gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc
aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct
ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
420 tgcatctaga tccatggctc tctcccttac taccgagcag ctgctcgagc
gacccgacct 480 ggttgccatc gacggcattc tctacgatct ggaaggtctt
gccaaggtcc atcccggagg 540 cgacttgatc ctcgcttctg gtgcctccga
tgcttctcct ctgttctact ccatgcaccc 600 ttacgtcaag cccgagaact
cgaagctgct tcaacagttc gtgcgaggca agcacgaccg 660 aacctccaag
gacattgtct acacctacga ctctcccttt gcacaggacg tcaagcgaac 720
tatgcgagag gtcatgaaag gtcggaactg gtatgccaca cctggattct ggctgcgaac
780 cgttggcatc attgctgtca ccgccttttg cgagtggcac tgggctacta
ccggaatggt 840 gctgtggggt ctcttgactg gattcatgca catgcagatc
ggcctgtcca ttcagcacga 900 tgcctctcat ggtgccatca gcaaaaagcc
ctgggtcaac gctctctttg cctacggcat 960 cgacgtcatt ggatcgtcca
gatggatctg gctgcagtct cacatcatgc gacatcacac 1020 ctacaccaat
cagcatggtc tcgacctgga tgccgagtcc gcagaaccat tccttgtgtt 1080
ccacaactac cctgctgcca acactgctcg aaagtggttt caccgattcc aggcctggta
1140 catgtacctc gtgcttggag cctacggcgt ttcgctggtg tacaaccctc
tctacatctt 1200 ccgaatgcag cacaacgaca ccattcccga gtctgtcaca
gccatgcgag agaacggctt 1260 tctgcgacgg taccgaaccc ttgcattcgt
tatgcgagct ttcttcatct ttcgaaccgc 1320 cttcttgccc tggtatctca
ctggaacctc cctgctcatc accattcctc tggtgcccac 1380 tgctaccggt
gccttcctca ccttcttttt catcttgtct cacaacttcg atggctcgga 1440
gcgaatcccc gacaagaact gcaaggtcaa gagctccgag aaggacgttg aagccgatca
1500 gatcgactgg tacagagctc aggtggagac ctcttccacc tacggtggac
ccattgccat 1560 gttctttact ggcggtctca acttccagat cgagcatcac
ctctttcctc gaatgtcgtc 1620 ttggcactat cccttcgtgc agcaagctgt
ccgagagtgt tgcgaacgac acggagttcg 1680 gtacgtcttc taccctacca
ttgtgggcaa catcatttcc accctcaagt acatgcacaa 1740 agtcggtgtg
gttcactgtg tcaaggacgc tcaggattcc taagcggccg catcggatcc 1800
cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt
1860 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
gaagcataaa 1920 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
ttaattgcgt tgcgctcact 1980 gcccgctttc cagtcgggaa acctgtcgtg
ccagctgcat taatgaatcg gccaacgcgc 2040 ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 2100 ctcggtcgtt
cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2160
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
2220 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
ctgacgagca 2280 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
acaggactat aaagatacca 2340 ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt ccgaccctgc cgcttaccgg 2400 atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2460 gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2520
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2580 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
ggtatgtagg 2640 cggtgctaca gagttcttga agtggtggcc taactacggc
tacactagaa gaacagtatt 2700 tggtatctgc gctctgctga agccagttac
cttcggaaaa agagttggta gctcttgatc 2760 cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 2820 cagaaaaaaa
ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 2880
gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2940 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
agtaaacttg 3000 gtctgacagt taccaatgct taatcagtga ggcacctatc
tcagcgatct gtctatttcg 3060 ttcatccata gttgcctgac tccccgtcgt
gtagataact acgatacggg agggcttacc 3120 atctggcccc agtgctgcaa
tgataccgcg agacccacgc tcaccggctc cagatttatc 3180 agcaataaac
cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 3240
ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
3300 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
cgtttggtat 3360 ggcttcattc agctccggtt cccaacgatc aaggcgagtt
acatgatccc ccatgttgtg 3420 caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc agaagtaagt tggccgcagt 3480 gttatcactc atggttatgg
cagcactgca taattctctt actgtcatgc catccgtaag 3540 atgcttttct
gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 3600
accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
3660 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
tcttaccgct 3720 gttgagatcc agttcgatgt aacccactcg tgcacccaac
tgatcttcag catcttttac 3780 tttcaccagc gtttctgggt gagcaaaaac
aggaaggcaa aatgccgcaa aaaagggaat 3840 aagggcgaca cggaaatgtt
gaatactcat actcttcctt tttcaatatt attgaagcat 3900 ttatcagggt
tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 3960
aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
4020 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 4070
<210> SEQ ID NO 49 <211> LENGTH: 8438 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pDMW369 <400>
SEQUENCE: 49 ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt
ggcaatccaa 60 gatggatgga ttcaacacag ggatatagcg agctacgtgg
tggtgcgagg atatagcaac 120 ggatatttat gtttgacact tgagaatgta
cgatacaagc actgtccaag tacaatacta 180 aacatactgt acatactcat
actcgtaccc gggcaacggt ttcacttgag tgcagtggct 240 agtgctctta
ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat 300
tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
360 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
tcgggaaacc 420 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
gagaggcggt ttgcgtattg 480 ggcgctcttc cgcttcctcg ctcactgact
cgctgcgctc ggtcgttcgg ctgcggcgag 540 cggtatcagc tcactcaaag
gcggtaatac ggttatccac agaatcaggg gataacgcag 600 gaaagaacat
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 660
tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
720 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
ggaagctccc 780 tcgtgcgctc tcctgttccg accctgccgc ttaccggata
cctgtccgcc tttctccctt 840 cgggaagcgt ggcgctttct catagctcac
gctgtaggta tctcagttcg gtgtaggtcg 900 ttcgctccaa gctgggctgt
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 960 ccggtaacta
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 1020
ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
1080 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
ctgctgaagc 1140 cagttacctt cggaaaaaga gttggtagct cttgatccgg
caaacaaacc accgctggta 1200 gcggtggttt ttttgtttgc aagcagcaga
ttacgcgcag aaaaaaagga tctcaagaag 1260 atcctttgat cttttctacg
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 1320 ttttggtcat
gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 1380
gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
1440 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
gcctgactcc 1500 ccgtcgtgta gataactacg atacgggagg gcttaccatc
tggccccagt gctgcaatga 1560 taccgcgaga cccacgctca ccggctccag
atttatcagc aataaaccag ccagccggaa 1620 gggccgagcg cagaagtggt
cctgcaactt tatccgcctc catccagtct attaattgtt 1680 gccgggaagc
tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 1740
ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
1800 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
agctccttcg 1860 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
atcactcatg gttatggcag 1920 cactgcataa ttctcttact gtcatgccat
ccgtaagatg cttttctgtg actggtgagt 1980 actcaaccaa gtcattctga
gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 2040 caatacggga
taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 2100
gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
2160 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
tctgggtgag 2220 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
ggcgacacgg aaatgttgaa 2280 tactcatact cttccttttt caatattatt
gaagcattta tcagggttat tgtctcatga 2340 gcggatacat atttgaatgt
atttagaaaa ataaacaaat aggggttccg cgcacatttc 2400 cccgaaaagt
gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 2460
ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
2520 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
cgggggctcc 2580 ctttagggtt ccgatttagt gctttacggc acctcgaccc
caaaaaactt gattagggtg 2640 atggttcacg tagtgggcca tcgccctgat
agacggtttt tcgccctttg acgttggagt 2700 ccacgttctt taatagtgga
ctcttgttcc aaactggaac aacactcaac cctatctcgg 2760 tctattcttt
tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 2820
tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc
2880 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
cgctattacg 2940 ccagctggcg aaagggggat gtgctgcaag gcgattaagt
tgggtaacgc cagggttttc 3000 ccagtcacga cgttgtaaaa cgacggccag
tgaattgtaa tacgactcac tatagggcga 3060 attgggtacc gggccccccc
tcgaggtcga tggtgtcgat aagcttgata tcgaattcat 3120 gtcacacaaa
ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag 3180
atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata
3240 ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca
ttgctaaata 3300 gacagactcc atctgccgcc tccaactgat gttctcaata
tttaaggggt catctcgcat 3360 tgtttaataa taaacagact ccatctaccg
cctccaaatg atgttctcaa aatatattgt 3420 atgaacttat ttttattact
tagtattatt agacaactta cttgctttat gaaaaacact 3480 tcctatttag
gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa 3540
atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc
3600 ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata
gtcatcgaga 3660 aatatcaact atcaaagaac agctattcac acgttactat
tgagattatt attggacgag 3720 aatcacacac tcaactgtct ttctctcttc
tagaaataca ggtacaagta tgtactattc 3780 tcattgttca tacttctagt
catttcatcc cacatattcc ttggatttct ctccaatgaa 3840 tgacattcta
tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt 3900
ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt
3960 aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg
gctggataca 4020 taaaggtatt ttgatttaat tttttgctta aattcaatcc
cccctcgttc agtgtcaact 4080 gtaatggtag gaaattacca tacttttgaa
gaagcaaaaa aaatgaaaga aaaaaaaaat 4140 cgtatttcca ggttagacgt
tccgcagaat ctagaatgcg gtatgcggta cattgttctt 4200 cgaacgtaaa
agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta 4260
catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg
4320 tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact
tgtagtaagc 4380 cgggttattg gcgttcaatt aatcatagac ttatgaatct
gcacggtgtg cgctgcgagt 4440 tacttttagc ttatgcatgc tacttgggtg
taatattggg atctgttcgg aaatcaacgg 4500 atgctcaatc gatttcgaca
gtaattaatt aagtcataca caagtcagct ttcttcgagc 4560 ctcatataag
tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga 4620
aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata
4680 catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc
tccatacttg 4740 cacgctctct atatacacag ttaaattaca tatccatagt
ctaacctcta acagttaatc 4800 ttctggtaag cctcccagcc agccttctgg
tatcgcttgg cctcctcaat aggatctcgg 4860 ttctggccgt acagacctcg
gccgacaatt atgatatccg ttccggtaga catgacatcc 4920 tcaacagttc
ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg 4980
gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc
5040 acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc
gccagtggcc 5100 agagagccct tgcaagacag ctcggccagc atgagcagac
ctctggccag cttctcgttg 5160 ggagagggga ctaggaactc cttgtactgg
gagttctcgt agtcagagac gtcctccttc 5220 ttctgttcag agacagtttc
ctcggcacca gctcgcaggc cagcaatgat tccggttccg 5280 ggtacaccgt
gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg 5340
tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc
5400 ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
gtcaatgatg 5460 tcgatatggg ttttgatcat gcacacataa ggtccgacct
tatcggcaag ctcaatgagc 5520 tccttggtgg tggtaacatc cagagaagca
cacaggttgg ttttcttggc tgccacgagc 5580 ttgagcactc gagcggcaaa
ggcggacttg tggacgttag ctcgagcttc gtaggagggc 5640 attttggtgg
tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc 5700
ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga
5760 tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
tctctgggcg 5820 tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag
caatgacgtt gcagctgata 5880 ttgttgtcgg ccaaccgcgc cgaaaacgca
gctgtcagac ccacagcctc caacgaagaa 5940 tgtatcgtca aagtgatcca
agcacactca tagttggagt cgtactccaa aggcggcaat 6000 gacgagtcag
acagatactc gtcgactcag gcgacgacgg aattcctgca gcccatctgc 6060
agaattcagg agagaccggg ttggcggcgt atttgtgtcc caaaaaacag ccccaattgc
6120 cccggagaag acggccaggc cgcctagatg acaaattcaa caactcacag
ctgactttct 6180 gccattgcca ctaggggggg gcctttttat atggccaagc
caagctctcc acgtcggttg 6240 ggctgcaccc aacaataaat gggtagggtt
gcaccaacaa agggatggga tggggggtag 6300 aagatacgag gataacgggg
ctcaatggca caaataagaa cgaatactgc cattaagact 6360 cgtgatccag
cgactgacac cattgcatca tctaagggcc tcaaaactac ctcggaactg 6420
ctgcgctgat ctggacacca cagaggttcc gagcacttta ggttgcacca aatgtcccac
6480 caggtgcagg cagaaaacgc tggaacagcg tgtacagttt gtcttaacaa
aaagtgaggg 6540 cgctgaggtc gagcagggtg gtgtgacttg ttatagcctt
tagagctgcg aaagcgcgta 6600 tggatttggc tcatcaggcc agattgaggg
tctgtggaca catgtcatgt tagtgtactt 6660 caatcgcccc ctggatatag
ccccgacaat aggccgtggc ctcatttttt tgccttccgc 6720 acatttccat
tgctcggtac ccacaccttg cttctcctgc acttgccaac cttaatactg 6780
gtttacattg accaacatct tacaagcggg gggcttgtct agggtatata taaacagtgg
6840 ctctcccaat cggttgccag tctctttttt cctttctttc cccacagatt
cgaaatctaa 6900 actacacatc acacaatgcc tgttactgac gtccttaagc
gaaagtccgg tgtcatcgtc 6960 ggcgacgatg tccgagccgt gagtatccac
gacaagatca gtgtcgagac gacgcgtttt 7020 gtgtaatgac acaatccgaa
agtcgctagc aacacacact ctctacacaa actaacccag 7080 ctctccatgg
ctctctccct tactaccgag cagctgctcg agcgacccga cctggttgcc 7140
atcgacggca ttctctacga tctggaaggt cttgccaagg tccatcccgg aggcgacttg
7200 atcctcgctt ctggtgcctc cgatgcttct cctctgttct actccatgca
cccttacgtc 7260 aagcccgaga actcgaagct gcttcaacag ttcgtgcgag
gcaagcacga ccgaacctcc 7320 aaggacattg tctacaccta cgactctccc
tttgcacagg acgtcaagcg aactatgcga 7380 gaggtcatga aaggtcggaa
ctggtatgcc acacctggat tctggctgcg aaccgttggc 7440 atcattgctg
tcaccgcctt ttgcgagtgg cactgggcta ctaccggaat ggtgctgtgg 7500
ggtctcttga ctggattcat gcacatgcag atcggcctgt ccattcagca cgatgcctct
7560 catggtgcca tcagcaaaaa gccctgggtc aacgctctct ttgcctacgg
catcgacgtc 7620 attggatcgt ccagatggat ctggctgcag tctcacatca
tgcgacatca cacctacacc 7680 aatcagcatg gtctcgacct ggatgccgag
tccgcagaac cattccttgt gttccacaac 7740 taccctgctg ccaacactgc
tcgaaagtgg tttcaccgat tccaggcctg gtacatgtac 7800 ctcgtgcttg
gagcctacgg cgtttcgctg gtgtacaacc ctctctacat cttccgaatg 7860
cagcacaacg acaccattcc cgagtctgtc acagccatgc gagagaacgg ctttctgcga
7920 cggtaccgaa cccttgcatt cgttatgcga gctttcttca tctttcgaac
cgccttcttg 7980 ccctggtatc tcactggaac ctccctgctc atcaccattc
ctctggtgcc cactgctacc 8040 ggtgccttcc tcaccttctt tttcatcttg
tctcacaact tcgatggctc ggagcgaatc 8100 cccgacaaga actgcaaggt
caagagctcc gagaaggacg ttgaagccga tcagatcgac 8160 tggtacagag
ctcaggtgga gacctcttcc acctacggtg gacccattgc catgttcttt 8220
actggcggtc tcaacttcca gatcgagcat cacctctttc ctcgaatgtc gtcttggcac
8280 tatcccttcg tgcagcaagc tgtccgagag tgttgcgaac gacacggagt
tcggtacgtc 8340 ttctacccta ccattgtggg caacatcatt tccaccctca
agtacatgca caaagtcggt 8400 gtggttcact gtgtcaagga cgctcaggat
tcctaagc 8438 <210> SEQ ID NO 50 <211> LENGTH: 19
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer T7
<400> SEQUENCE: 50 ggaaacagct atgaccatg 19 <210> SEQ ID
NO 51 <211> LENGTH: 459 <212> TYPE: PRT <213>
ORGANISM: Rhizopus stolonifer <220> FEATURE: GenBank
Accession No. AAX22052 <221> NAME/KEY: MISC_FEATURE
<223> OTHER INFORMATION: delta-6 desaturase <400>
SEQUENCE: 51 Met Ser Thr Leu Asp Arg Gln Ser Ile Phe Thr Ile Lys
Glu Leu Glu 1 5 10 15 Ser Ile Ser Gln Arg Ile His Asp Gly Asp Glu
Glu Ala Met Lys Phe 20 25 30 Ile Ile Ile Asp Lys Lys Val Tyr Asp
Val Thr Glu Phe Ile Glu Asp 35 40 45 His Pro Gly Gly Ala Gln Val
Leu Leu Thr His Val Gly Lys Asp Ala 50 55 60 Ser Asp Val Phe His
Ala Met His Pro Glu Ser Ala Tyr Glu Val Leu 65 70 75 80 Asn Asn Tyr
Phe Val Gly Asp Val Gln Glu Thr Val Val Thr Glu Lys 85 90 95 Ser
Ser Ser Ala Gln Phe Ala Val Glu Met Arg Gln Leu Arg Asp Gln 100 105
110 Leu Lys Lys Glu Gly Tyr Phe His Ser Ser Lys Leu Phe Tyr Ala Tyr
115 120 125 Lys Val Leu Ser Thr Leu Ala Ile Cys Ile Ala Gly Leu Ser
Leu Leu 130 135 140 Tyr Ala Tyr Gly Arg Thr Ser Thr Leu Ala Val Val
Ala Ser Ala Ile 145 150 155 160 Thr Val Gly Ile Phe Trp Gln Gln Cys
Gly Trp Leu Ala His Asp Phe 165 170 175 Gly His His Gln Cys Phe Glu
Asp Arg Thr Trp Asn Asp Val Leu Val 180 185 190 Val Phe Leu Gly Asn
Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp Lys 195 200 205 Asn Lys His
Asn Thr His His Ala Ser Thr Asn Val His Gly Gln Asp 210 215 220 Pro
Asp Ile Asp Thr Ala Pro Val Leu Leu Trp Asp Glu Tyr Ala Ser 225 230
235 240 Ala Ala Tyr Tyr Ala Ser Leu Asp Gln Glu Pro Thr Met Val Ser
Arg 245 250 255 Phe Leu Ala Glu Gln Val Leu Pro His Gln Thr Arg Tyr
Phe Phe Phe 260 265 270 Ile Leu Ala Phe Ala Arg Leu Ser Trp Ala Leu
Gln Ser Leu Ser Tyr 275 280 285 Ser Phe Lys Lys Glu Ser Ile Asn Lys
Ser Arg Gln Leu Asn Leu Phe 290 295 300 Glu Arg Val Cys Ile Val Gly
His Trp Ala Leu Phe Ala Phe Cys Ile 305 310 315 320 Tyr Ser Trp Cys
Ser Asn Val Tyr His Met Val Leu Phe Phe Leu Val 325 330 335 Ser Gln
Ala Thr Thr Gly Tyr Thr Leu Ala Leu Val Phe Ala Leu Asn 340 345 350
His Asn Gly Met Pro Val Ile Thr Glu Glu Lys Ala Glu Ser Met Glu 355
360 365 Phe Phe Glu Ile Gln Val Ile Thr Gly Arg Asp Val Thr Leu Ser
Pro 370 375 380 Leu Gly Asp Trp Phe Met Gly Gly Leu Asn Tyr Gln Ile
Glu His His 385 390 395 400 Val Phe Pro Asn Met Pro Arg His Asn Leu
Pro Thr Val Lys Pro Met 405 410 415 Val Lys Ser Leu Cys Gln Lys Tyr
Asp Ile Asn Tyr His Asp Thr Gly 420 425 430 Phe Leu Lys Gly Thr Leu
Glu Val Leu Gln Thr Leu Asp Ile Thr Ser 435 440 445 Lys Leu Ser Leu
Gln Leu Ser Lys Lys Ser Phe 450 455 <210> SEQ ID NO 52
<211> LENGTH: 695 <212> TYPE: DNA <213> ORGANISM:
Pavlova lutheri <400> SEQUENCE: 52 agggccaagg gtgccaacca
ccttccacgt gagactacac accgtaggcc gatgggcaag 60 ggtggagacg
gcggcgcgca ggcggtgagc gggaccgacg cgtctctcgc tgaggtgagc 120
tccgtcgata gcaagagcgt gcacgtcgtg ctctacggca agcgcgtgga tgtcacaaag
180 ttccagggct acgacgtggc ctggtggcgc gcgcgccata acacgcacca
cgtgtgcacc 240 aacgaggatg gttcggaccc ggacatcaag acggcgcccc
tgctcatcta cgtgcgagag 300 aacccgtcca ttgccaagcg gctcaacttc
ttccagcgct ggcagcagta ctactatgtg 360 ccgaccatgg ccatcctcga
cctctactgg cgcctggagt ccatcgcgta cgtggctgtg 420 cgcctgccta
agatgtggat gcaggccgcc gctcttgccg ctcactacgc gctcctgtgc 480
tgggtcttcg cagcgcatct caacctcatc cctctcatga tggttgcacg cggcttcgcg
540 acgggcatcg ttgtctttgc aacccactat ggtgaggaca tcctcgaccg
cgagcacgtc 600 gagggcatga cgctcgtcga gcagaccgcc aagacctccc
gtaacatcac gggcggctgg 660 ctagtgaacg tgctcacggg cttcatctcc ctgca
695 <210> SEQ ID NO 53 <211> LENGTH: 1106 <212>
TYPE: DNA <213> ORGANISM: Pavlova lutheri <400>
SEQUENCE: 53 agggccaagg gtgccaacca ccttccacgt gagactacac accgtaggcc
gatgggcaag 60 ggtggagacg gcggcgcgca ggcggtgagc gggaccgacg
cgtctctcgc tgaggtgagc 120 tccgtcgata gcaagagcgt gcacgtcgtg
ctctacggca agcgcgtgga tgtcacaaag 180 ttccagggct acgacgtggc
ctggtggcgc gcgcgccata acacgcacca cgtgtgcacc 240 aacgaggatg
gttcggaccc ggacatcaag acggcgcccc tgctcatcta cgtgcgagag 300
aacccgtcca ttgccaagcg gctcaacttc ttccagcgct ggcagcagta ctactatgtg
360 ccgaccatgg ccatcctcga cctctactgg cgcctggagt ccatcgcgta
cgtggctgtg 420 cgcctgccta agatgtggat gcaggccgcc gctcttgccg
ctcactacgc gctcctgtgc 480 tgggtcttcg cagcgcatct caacctcatc
cctctcatga tggttgcacg cggcttcgcg 540 acgggcatcg ttgtctttgc
aacccactat ggtgaggaca tcctcgaccg cgagcacgtc 600 gagggcatga
cgctcgtcga gcagaccgcc aagacctccc gtaacatcac gggcggctgg 660
ctagtgaacg tgctcacggg cttcatctcc ctgcagaccg agcatcacct cttccccatg
720 atgcccaccg gcaacctaat gactatccag cccgaggtac gcgacttctt
caagaagcat 780 ggcctcgagt accgcgaggg caacctcttc cagtgcgtgc
accagaacat caaggctctc 840 gccttcgagc acctcctcca ctgagcgtca
ccactcaagc gtcctaagtg cacaggtact 900 gtcttctgac cgatggccgc
gcggctccct cggctggcag tggggccaac gagtggcctc 960 gcgggatcgg
gcacgatcgg gcctccatga aacttcagtg ttcagagaca agccgacaac 1020
ctccgcatcg tgagaaatct tttaaagcag tatgttccat cacgccgctt ttgcagtcaa
1080 taacattacc caaaaaaaaa aaaaaa 1106 <210> SEQ ID NO 54
<211> LENGTH: 287 <212> TYPE: PRT <213> ORGANISM:
Pavlova lutheri <400> SEQUENCE: 54 Arg Ala Lys Gly Ala Asn
His Leu Pro Arg Glu Thr Thr His Arg Arg 1 5 10 15 Pro Met Gly Lys
Gly Gly Asp Gly Gly Ala Gln Ala Val Ser Gly Thr 20 25 30 Asp Ala
Ser Leu Ala Glu Val Ser Ser Val Asp Ser Lys Ser Val His 35 40 45
Val Val Leu Tyr Gly Lys Arg Val Asp Val Thr Lys Phe Gln Gly Tyr 50
55 60 Asp Val Ala Trp Trp Arg Ala Arg His Asn Thr His His Val Cys
Thr 65 70 75 80 Asn Glu Asp Gly Ser Asp Pro Asp Ile Lys Thr Ala Pro
Leu Leu Ile 85 90 95 Tyr Val Arg Glu Asn Pro Ser Ile Ala Lys Arg
Leu Asn Phe Phe Gln 100 105 110 Arg Trp Gln Gln Tyr Tyr Tyr Val Pro
Thr Met Ala Ile Leu Asp Leu 115 120 125 Tyr Trp Arg Leu Glu Ser Ile
Ala Tyr Val Ala Val Arg Leu Pro Lys 130 135 140 Met Trp Met Gln Ala
Ala Ala Leu Ala Ala His Tyr Ala Leu Leu Cys 145 150 155 160 Trp Val
Phe Ala Ala His Leu Asn Leu Ile Pro Leu Met Met Val Ala 165 170 175
Arg Gly Phe Ala Thr Gly Ile Val Val Phe Ala Thr His Tyr Gly Glu 180
185 190 Asp Ile Leu Asp Arg Glu His Val Glu Gly Met Thr Leu Val Glu
Gln 195 200 205 Thr Ala Lys Thr Ser Arg Asn Ile Thr Gly Gly Trp Leu
Val Asn Val 210 215 220 Leu Thr Gly Phe Ile Ser Leu Gln Thr Glu His
His Leu Phe Pro Met 225 230 235 240 Met Pro Thr Gly Asn Leu Met Thr
Ile Gln Pro Glu Val Arg Asp Phe 245 250 255 Phe Lys Lys His Gly Leu
Glu Tyr Arg Glu Gly Asn Leu Phe Gln Cys 260 265 270 Val His Gln Asn
Ile Lys Ala Leu Ala Phe Glu His Leu Leu His 275 280 285 <210>
SEQ ID NO 55 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer SeqE <400> SEQUENCE: 55
cgacacactc caatctttcc 20 <210> SEQ ID NO 56 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
SeqW <400> SEQUENCE: 56 ggtggctgga gttagacatc 20 <210>
SEQ ID NO 57 <211> LENGTH: 22 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AP1 <400> SEQUENCE: 57
gtaatacgac tcactatagg gc 22 <210> SEQ ID NO 58 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
GSP PvDES <400> SEQUENCE: 58 ctgcgaagac ccagcacagg 20
<210> SEQ ID NO 59 <211> LENGTH: 22 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer M13-28Rev <400>
SEQUENCE: 59 gtaatacgac tcactatagg gc 22 <210> SEQ ID NO 60
<211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PavDes seq <400> SEQUENCE: 60 ttgtggcgct
caatcatctc c 21 <210> SEQ ID NO 61 <211> LENGTH: 1294
<212> TYPE: DNA <213> ORGANISM: Pavlova lutheri
<400> SEQUENCE: 61 ctttgcgagc gcggcgcaga cgattgcggc
ccgtagtgat cgcggtgcgc attgctgtgt 60 ttctagtttt gctgacgccc
ggcccgataa tgacaccttc tcccgtttga aatactaata 120 agtaactata
ttataatatt caaaggtggc gactatggat ctccttttct aaagttcagc 180
ggaattggga atcggagaaa tttcgagata tgtcataatc acgtgctcta tctcgaatga
240 accgcggccg gtgagcgatt actcgggaag ccaattccta ttaacgagtc
aggggggatc 300 tttgaggtga gtcggccacg cagagagagc aaggaatcat
cctcatccgc cgttctcgag 360 aaagagccaa gggtgccaac caccttccac
gtgagactac acaccgtagg ccgatgggca 420 agggtggaga cggcggcgcg
caggcggcga gcgggaccga cgcatctctc gctgaggtga 480 gctccgtcga
tagcaagagc gtgcgcgtcg tgctctacgg caagcgcgtg gatgtcacaa 540
agttccagag ggcacacccg ggcgggagca aggtgttccg catcttccag gagcgcgacg
600 cgacggagca gttcgagtct taccactcgc ccaaggccat caagatgatg
gagggcatgc 660 tcaagaagtc ggaggatgcg cccgcttccg tgcccctgcc
ctcgcggtcc accatgggca 720 cggagttcaa ggagatgatt gagcgccaca
agagggctgg tctctacgac ccttgcccgt 780 tggacgagct gttcaagctc
accatcgtcc ttgcgcccat cttcgtgggc gcctatctcg 840 tgcggagcgg
cgtctcgccc ctcgcgggcg cgctctccat gggctttggc ttctacctcg 900
acggctggct tgctcacrac tacctgcatc acgcagtctt caagggctcg gtcaacacgc
960 tcgtcaaggc gaacaacgcc atgggatacg ccctcggctt cctccagggc
tacgacgtgg 1020 cctggtggcg cgcgcgccat aacacgcacc acgtgtgcac
caacgaggat ggttcggacc 1080 cggacatcaa gacggcgccc ctgctcatct
acgtgcgaga gaacccgtcc attgccaagc 1140 ggctcaactt cttccagcgc
tggcagcagt actactatgt gccgaccatg gccatcctcg 1200 acctctactg
gcgcctggag tccatcgcgt acgtggctgt gcgcctgcct aagatgtgga 1260
tgcaggccgc cgctcttgcc gctcactacg cgct 1294 <210> SEQ ID NO 62
<211> LENGTH: 1927 <212> TYPE: DNA <213>
ORGANISM: Pavlova lutheri <400> SEQUENCE: 62 ctttgcgagc
gcggcgcaga cgattgcggc ccgtagtgat cgcggtgcgc attgctgtgt 60
ttctagtttt gctgacgccc ggcccgataa tgacaccttc tcccgtttga aatactaata
120 agtaactata ttataatatt caaaggtggc gactatggat ctccttttct
aaagttcagc 180 ggaattggga atcggagaaa tttcgagata tgtcataatc
acgtgctcta tctcgaatga 240 accgcggccg gtgagcgatt actcgggaag
ccaattccta ttaacgagtc aggggggatc 300 tttgaggtga gtcggccacg
cagagagagc aaggaatcat cctcatccgc cgttctcgag 360 aaagagccaa
gggtgccaac caccttccac gtgagactac acaccgtagg ccgatgggca 420
agggtggaga cggcggcgcg caggcggtga gcgggaccga cgcgtctctc gctgaggtga
480 gctccgtcga tagcaagagc gtgcacgtcg tgctctacgg caagcgcgtg
gatgtcacaa 540 agttccagaa ggcacacccg ggcgggagca aggtgttccg
catcttccag gagcgcgacg 600 cgacggagca gttcgagtct taccactcgc
ccaaggccat caagatgatg gagggcatgc 660 tcaagaagtc ggaggatgcg
cccgcttccg tgcccctgcc ctcgcggtcc accatgggca 720 cggagttcaa
ggagatgatt gagcgccaca agagggctgg tctctacgac ccttgcccgt 780
tggacgagct gttcaagctc accatcgtcc ttgcgcccat cttcgtgggc gcctatctcg
840 tgcggagcgg cgtctcgccc ctcgcgggcg cgctctccat gggctttggc
ttctacctcg 900 acggctggct tgctcacgac tacctgcatc acgcagtctt
caagggctcg gtcaacacgc 960 tcgtcaaggc gaacaacgcc atgggatacg
ccctcggctt cctccagggc tacgacgtgg 1020 cctggtggcg cgcgcgccat
aacacgcacc acgtgtgcac caacgaggat ggttcggacc 1080 cggacatcaa
gacggcgccc ctgctcatct acgtgcgaga gaacccgtcc attgccaagc 1140
ggctcaactt cttccagcgc tggcagcagt actactatgt gccgaccatg gccatcctcg
1200 acctctactg gcgcctggag tccatcgcgt acgtggctgt gcgcctgcct
aagatgtgga 1260 tgcaggccgc cgctcttgcc gctcactacg cgctcctgtg
ctgggtcttc gcagcgcatc 1320 tcaacctcat ccctctcatg atggttgcac
gcggcttcgc gacgggcatc gttgtctttg 1380 caacccacta tggtgaggac
atcctcgacc gcgagcacgt cgagggcatg acgctcgtcg 1440 agcagaccgc
caagacctcc cgtaacatca cgggcggctg gctagtgaac gtgctcacgg 1500
gcttcatctc cctgcagacc gagcatcacc tcttccccat gatgcccacc ggcaacctaa
1560 tgactatcca gcccgaggta cgcgacttct tcaagaagca tggcctcgag
taccgcgagg 1620 gcaacctctt ccagtgcgtg caccagaaca tcaaggctct
cgccttcgag cacctcctcc 1680 actgagcgtc accactcaag cgtcctaagt
gcacaggtac tgtcttctga ccgatggccg 1740 cgcggctccc tcggctggca
gtggggccaa cgagtggcct cgcgggatcg ggcacgatcg 1800 ggcctccatg
aaacttcagt gttcagagac aagccgacaa cctccgcatc gtgagaaatc 1860
ttttaaagca gtatgttcca tcacgccgct tttgcagtca ataacattac ccaaaaaaaa
1920 aaaaaaa 1927 <210> SEQ ID NO 63 <211> LENGTH: 459
<212> TYPE: PRT <213> ORGANISM: Rhizopus stolonifer
<220> FEATURE: GenBank Accession No. ABB96724 <221>
NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION: delta-6
destaurase <400> SEQUENCE: 63 Met Ser Thr Leu Asp Arg Gln Ser
Ile Phe Thr Ile Lys Glu Leu Glu 1 5 10 15 Ser Ile Ser Gln Arg Ile
His Asp Gly Asp Glu Glu Ala Met Lys Phe 20 25 30 Ile Ile Ile Asp
Lys Lys Val Tyr Asp Val Thr Glu Phe Ile Glu Asp 35 40 45 His Pro
Gly Gly Ala Gln Val Leu Leu Thr His Val Gly Lys Asp Ala 50 55 60
Ser Asp Val Phe His Ala Met His Pro Glu Ser Ala Tyr Glu Val Leu 65
70 75 80 Asn Asn Tyr Phe Val Gly Asp Val Gln Glu Thr Val Val Thr
Glu Lys 85 90 95 Ser Ser Ser Ala Gln Phe Ala Val Glu Met Arg Gln
Leu Arg Asp Gln 100 105 110 Leu Lys Lys Glu Gly Tyr Phe His Ser Ser
Lys Leu Phe Tyr Ala Tyr 115 120 125 Lys Val Leu Ser Thr Leu Ala Ile
Cys Ile Ala Gly Leu Ser Pro Leu 130 135 140 Tyr Ala Tyr Gly Arg Thr
Ser Thr Leu Ala Val Val Ala Ser Ala Ile 145 150 155 160 Thr Val Gly
Ile Phe Trp Gln Gln Cys Gly Trp Leu Ala His Asp Phe 165 170 175 Gly
His His Gln Cys Phe Glu Asp Arg Thr Trp Asn Asp Val Leu Val 180 185
190 Val Phe Leu Gly Asn Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp Lys
195 200 205 Asn Lys His Asn Thr His His Ala Ser Thr Asn Val His Gly
Gln Asp 210 215 220 Pro Asp Ile Asp Thr Ala Pro Val Leu Leu Trp Asp
Glu Tyr Ala Ser 225 230 235 240 Ala Ala Tyr Tyr Ala Ser Leu Asp Gln
Glu Pro Thr Met Val Ser Arg 245 250 255 Phe Leu Ala Glu Gln Val Leu
Pro His Gln Thr Arg Tyr Phe Phe Phe 260 265 270 Ile Leu Ala Phe Ala
Arg Leu Ser Trp Ala Leu Gln Ser Leu Ser Tyr 275 280 285 Ser Phe Lys
Lys Glu Ser Ile Asn Lys Ser Arg Gln Leu Asn Leu Phe 290 295 300 Glu
Arg Val Cys Ile Val Gly His Trp Ala Leu Ser Ala Phe Cys Ile 305 310
315 320 Tyr Ser Trp Cys Ser Asn Val Tyr His Met Val Leu Phe Phe Leu
Val 325 330 335 Ser Gln Ala Thr Thr Gly Tyr Thr Leu Ala Leu Val Phe
Ala Leu Asn 340 345 350 His Asn Gly Met Pro Val Ile Thr Glu Glu Lys
Ala Glu Ser Met Glu 355 360 365 Phe Phe Glu Ile Gln Val Ile Thr Gly
Arg Asp Val Thr Leu Ser Pro 370 375 380 Leu Gly Asp Trp Phe Met Gly
Gly Leu Asn Tyr Gln Ile Glu His His 385 390 395 400 Val Phe Pro Asn
Met Pro Arg His Asn Leu Pro Thr Val Lys Pro Met 405 410 415 Val Lys
Ser Leu Cys Gln Lys Tyr Asp Ile Asn Tyr His Asp Thr Gly 420 425 430
Phe Leu Lys Gly Thr Leu Glu Val Leu Gln Thr Leu Asp Ile Thr Ser 435
440 445 Lys Leu Ser Leu Gln Leu Ser Lys Lys Ser Phe 450 455
<210> SEQ ID NO 64 <211> LENGTH: 427 <212> TYPE:
PRT <213> ORGANISM: Pavlova salina <300> PUBLICATION
INFORMATION: <302> TITLE: SYNTHESIS OF LONG-CHAIN
POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELLS <310> PATENT
DOCUMENT NUMBER: WO 2005/103253 <311> PATENT FILING DATE:
2005-04-22 <312> PUBLICATION DATE: 2005-11-03 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(427) <400> SEQUENCE: 64
Met Gly Arg Gly Gly Asp Ser Ser Gly Gln Ala His Pro Ala Ala Glu 1 5
10 15 Leu Ala Val Pro Ser Asp Arg Ala Glu Val Ser Asn Ala Asp Ser
Lys 20 25 30 Ala Leu His Ile Val Leu Tyr Gly Lys Arg Val Asp Val
Thr Lys Phe 35 40 45 Gln Arg Thr His Pro Gly Gly Ser Lys Val Phe
Arg Ile Phe Gln Asp 50 55 60 Arg Asp Ala Thr Glu Gln Phe Glu Ser
Tyr His Ser Lys Arg Ala Ile 65 70 75 80 Lys Met Met Glu Gly Met Leu
Lys Lys Ser Glu Asp Ala Pro Ala Asp 85 90 95 Thr Pro Leu Pro Ser
Gln Ser Pro Met Gly Lys Asp Phe Lys Ala Met 100 105 110 Ile Glu Arg
His Val Ala Ala Gly Tyr Tyr Asp Pro Cys Pro Leu Asp 115 120 125 Glu
Leu Phe Lys Leu Ser Leu Val Leu Leu Pro Thr Phe Ala Gly Met 130 135
140 Tyr Met Leu Lys Ala Gly Val Gly Ser Pro Leu Cys Gly Ala Leu Met
145 150 155 160 Val Ser Phe Gly Trp Tyr Leu Asp Gly Trp Leu Ala His
Asp Tyr Leu 165 170 175 His His Ser Val Phe Lys Gly Ser Val Ala Arg
Thr Val Gly Trp Asn 180 185 190 Asn Ala Ala Gly Tyr Phe Leu Gly Phe
Val Gln Gly Tyr Ala Val Glu 195 200 205 Trp Trp Arg Ala Arg His Asn
Thr His His Val Cys Thr Asn Glu Asp 210 215 220 Gly Ser Asp Pro Asp
Ile Lys Thr Ala Pro Leu Leu Ile Tyr Val Arg 225 230 235 240 Asn Lys
Pro Ser Ile Ala Lys Arg Leu Asn Ala Phe Gln Arg Tyr Gln 245 250 255
Gln Tyr Tyr Tyr Val Pro Val Met Ala Ile Leu Asp Leu Tyr Trp Arg 260
265 270 Leu Glu Ser Ile Ala Tyr Val Ala Met Arg Leu Pro Lys Met Leu
Pro 275 280 285 Gln Ala Leu Ala Leu Val Ala His Tyr Ala Ile Val Ala
Trp Val Phe 290 295 300 Ala Gly Asn Tyr His Leu Leu Pro Leu Val Thr
Val Leu Arg Gly Phe 305 310 315 320 Gly Thr Gly Ile Thr Val Phe Ala
Thr His Tyr Gly Glu Asp Ile Leu 325 330 335 Asp Ala Asp Gln Val Arg
His Met Thr Leu Val Glu Gln Thr Ala Leu 340 345 350 Thr Ser Arg Asn
Ile Ser Gly Gly Trp Leu Val Asn Val Leu Thr Gly 355 360 365 Phe Ile
Ser Leu Gln Thr Glu His His Leu Phe Pro Met Met Pro Thr 370 375 380
Gly Asn Leu Met Thr Ile Gln Pro Glu Val Arg Ala Phe Phe Lys Lys 385
390 395 400 His Gly Leu Glu Tyr Arg Glu Gly Asn Leu Ile Glu Cys Val
Arg Gln 405 410 415 Asn Ile Arg Ala Leu Ala Phe Glu His Leu Leu 420
425 <210> SEQ ID NO 65 <211> LENGTH: 37 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Primer AP <400>
SEQUENCE: 65 ggccacgcgt cgactagtac tttttttttt ttttttt 37
<210> SEQ ID NO 66 <211> LENGTH: 39 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Smart IV oligonucleotide primer
<400> SEQUENCE: 66 aagcagtggt atcaacgcag agtggccatt acggccggg
39 <210> SEQ ID NO 67 <211> LENGTH: 1338 <212>
TYPE: DNA <213> ORGANISM: Mortierella alpina <400>
SEQUENCE: 67 atgggaacgg accaaggaaa aaccttcacc tgggaagagc tggcggccca
taacaccaag 60 gacgacctac tcttggccat ccgcggcagg gtgtacgatg
tcacaaagtt cttgagccgc 120 catcctggtg gagtggacac tctcctgctc
ggagctggcc gagatgttac tccggtcttt 180 gagatgtatc acgcgtttgg
ggctgcagat gccattatga agaagtacta tgtcggtaca 240 ctggtctcga
atgagctgcc catcttcccg gagccaacgg tgttccacaa aaccatcaag 300
acgagagtcg agggctactt tacggatcgg aacattgatc ccaagaatag accagagatc
360 tggggacgat acgctcttat ctttggatcc ttgatcgctt cctactacgc
gcagctcttt 420 gtgcctttcg ttgtcgaacg cacatggctt caggtggtgt
ttgcaatcat catgggattt 480 gcgtgcgcac aagtcggact caaccctctt
catgatgcgt ctcacttttc agtgacccac 540 aaccccactg tctggaagat
tctgggagcc acgcacgact ttttcaacgg agcatcgtac 600 ctggtgtgga
tgtaccaaca tatgctcggc catcacccct acaccaacat tgctggagca 660
gatcccgacg tgtcgacgtc tgagcccgat gttcgtcgta tcaagcccaa ccaaaagtgg
720 tttgtcaacc acatcaacca gcacatgttt gttcctttcc tgtacggact
gctggcgttc 780 aaggtgcgca ttcaggacat caacattttg tactttgtca
agaccaatga cgctattcgt 840 gtcaatccca tctcgacatg gcacactgtg
atgttctggg gcggcaaggc tttctttgtc 900 tggtatcgcc tgattgttcc
cctgcagtat ctgcccctgg gcaaggtgct gctcttgttc 960 acggtcgcgg
acatggtgtc gtcttactgg ctggcgctga ccttccaggc gaaccacgtt 1020
gttgaggaag ttcagtggcc gttgcctgac gagaacggga tcatccaaaa ggactgggca
1080 gctatgcagg tcgagactac gcaggattac gcacacgatt cgcacctctg
gaccagcatc 1140 actggcagct tgaactacca ggctgtgcac catctgttcc
ccaacgtgtc gcagcaccat 1200 tatcccgata ttctggccat catcaagaac
acctgcagcg agtacaaggt tccatacctt 1260 gtcaaggata cgttttggca
agcatttgct tcacatttgg agcacttgcg tgttcttgga 1320 ctccgtccca
aggaagag 1338 <210> SEQ ID NO 68 <211> LENGTH: 446
<212> TYPE: PRT <213> ORGANISM: Mortierella alpina
<400> SEQUENCE: 68 Met Gly Thr Asp Gln Gly Lys Thr Phe Thr
Trp Glu Glu Leu Ala Ala 1 5 10 15 His Asn Thr Lys Asp Asp Leu Leu
Leu Ala Ile Arg Gly Arg Val Tyr 20 25 30 Asp Val Thr Lys Phe Leu
Ser Arg His Pro Gly Gly Val Asp Thr Leu 35 40 45 Leu Leu Gly Ala
Gly Arg Asp Val Thr Pro Val Phe Glu Met Tyr His 50 55 60 Ala Phe
Gly Ala Ala Asp Ala Ile Met Lys Lys Tyr Tyr Val Gly Thr 65 70 75 80
Leu Val Ser Asn Glu Leu Pro Ile Phe Pro Glu Pro Thr Val Phe His 85
90 95 Lys Thr Ile Lys Thr Arg Val Glu Gly Tyr Phe Thr Asp Arg Asn
Ile 100 105 110 Asp Pro Lys Asn Arg Pro Glu Ile Trp Gly Arg Tyr Ala
Leu Ile Phe 115 120 125 Gly Ser Leu Ile Ala Ser Tyr Tyr Ala Gln Leu
Phe Val Pro Phe Val 130 135 140 Val Glu Arg Thr Trp Leu Gln Val Val
Phe Ala Ile Ile Met Gly Phe 145 150 155 160 Ala Cys Ala Gln Val Gly
Leu Asn Pro Leu His Asp Ala Ser His Phe 165 170 175 Ser Val Thr His
Asn Pro Thr Val Trp Lys Ile Leu Gly Ala Thr His 180 185 190 Asp Phe
Phe Asn Gly Ala Ser Tyr Leu Val Trp Met Tyr Gln His Met 195 200 205
Leu Gly His His Pro Tyr Thr Asn Ile Ala Gly Ala Asp Pro Asp Val 210
215 220 Ser Thr Ser Glu Pro Asp Val Arg Arg Ile Lys Pro Asn Gln Lys
Trp 225 230 235 240 Phe Val Asn His Ile Asn Gln His Met Phe Val Pro
Phe Leu Tyr Gly 245 250 255 Leu Leu Ala Phe Lys Val Arg Ile Gln Asp
Ile Asn Ile Leu Tyr Phe 260 265 270 Val Lys Thr Asn Asp Ala Ile Arg
Val Asn Pro Ile Ser Thr Trp His 275 280 285 Thr Val Met Phe Trp Gly
Gly Lys Ala Phe Phe Val Trp Tyr Arg Leu 290 295 300 Ile Val Pro Leu
Gln Tyr Leu Pro Leu Gly Lys Val Leu Leu Leu Phe 305 310 315 320 Thr
Val Ala Asp Met Val Ser Ser Tyr Trp Leu Ala Leu Thr Phe Gln 325 330
335 Ala Asn His Val Val Glu Glu Val Gln Trp Pro Leu Pro Asp Glu Asn
340 345 350 Gly Ile Ile Gln Lys Asp Trp Ala Ala Met Gln Val Glu Thr
Thr Gln 355 360 365 Asp Tyr Ala His Asp Ser His Leu Trp Thr Ser Ile
Thr Gly Ser Leu 370 375 380 Asn Tyr Gln Ala Val His His Leu Phe Pro
Asn Val Ser Gln His His 385 390 395 400 Tyr Pro Asp Ile Leu Ala Ile
Ile Lys Asn Thr Cys Ser Glu Tyr Lys 405 410 415 Val Pro Tyr Leu Val
Lys Asp Thr Phe Trp Gln Ala Phe Ala Ser His 420 425 430 Leu Glu His
Leu Arg Val Leu Gly Leu Arg Pro Lys Glu Glu 435 440 445 <210>
SEQ ID NO 69 <211> LENGTH: 6473 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY5-22 <400> SEQUENCE:
69 ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg
gcgtaatcat 60 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
aattccacac aacgtacgag 120 ccggaagcat aaagtgtaaa gcctggggtg
cctaatgagt gagctaactc acattaattg 180 cgttgcgctc actgcccgct
ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 240 tcggccaacg
cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 300
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
360 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
gcaaaaggcc 420 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
gtttttccat aggctccgcc 480 cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac 540 tataaagata ccaggcgttt
ccccctggaa gctccctcgt gcgctctcct gttccgaccc 600 tgccgcttac
cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 660
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
720 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
cttgagtcca 780 acccggtaag acacgactta tcgccactgg cagcagccac
tggtaacagg attagcagag 840 cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta 900 gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg 960 gtagctcttg
atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 1020
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
1080 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
ttatcaaaaa 1140 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
taaatcaatc taaagtatat 1200 atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga 1260 tctgtctatt tcgttcatcc
atagttgcct gactccccgt cgtgtagata actacgatac 1320 gggagggctt
accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 1380
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
1440 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
gtaagtagtt 1500 cgccagttaa tagtttgcgc aacgttgttg ccattgctac
aggcatcgtg gtgtcacgct 1560 cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat 1620 cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 1680 agttggccgc
agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 1740
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
1800 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
accgcgccac 1860 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
ttcggggcga aaactctcaa 1920 ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt 1980 cagcatcttt tactttcacc
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 2040 caaaaaaggg
aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 2100
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
2160 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
cctgacgcgc 2220 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
gcgcagcgtg accgctacac 2280 ttgccagcgc cctagcgccc gctcctttcg
ctttcttccc ttcctttctc gccacgttcg 2340 ccggctttcc ccgtcaagct
ctaaatcggg ggctcccttt agggttccga tttagtgctt 2400 tacggcacct
cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 2460
cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
2520 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
ttataaggga 2580 ttttgccgat ttcggcctat tggttaaaaa atgagctgat
ttaacaaaaa tttaacgcga 2640 attttaacaa aatattaacg cttacaattt
ccattcgcca ttcaggctgc gcaactgttg 2700 ggaagggcga tcggtgcggg
cctcttcgct attacgccag ctggcgaaag ggggatgtgc 2760 tgcaaggcga
ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 2820
ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga
2880 ggtcgatggt gtcgataagc ttgatatcga attcatgtca cacaaaccga
tcttcgcctc 2940 aaggaaacct aattctacat ccgagagact gccgagatcc
agtctacact gattaatttt 3000 cgggccaata atttaaaaaa atcgtgttat
ataatattat atgtattata tatatacatc 3060 atgatgatac tgacagtcat
gtcccattgc taaatagaca gactccatct gccgcctcca 3120 actgatgttc
tcaatattta aggggtcatc tcgcattgtt taataataaa cagactccat 3180
ctaccgcctc caaatgatgt tctcaaaata tattgtatga acttattttt attacttagt
3240 attattagac aacttacttg ctttatgaaa aacacttcct atttaggaaa
caatttataa 3300 tggcagttcg ttcatttaac aatttatgta gaataaatgt
tataaatgcg tatgggaaat 3360 cttaaatatg gatagcataa atgatatctg
cattgcctaa ttcgaaatca acagcaacga 3420 aaaaaatccc ttgtacaaca
taaatagtca tcgagaaata tcaactatca aagaacagct 3480 attcacacgt
tactattgag attattattg gacgagaatc acacactcaa ctgtctttct 3540
ctcttctaga aatacaggta caagtatgta ctattctcat tgttcatact tctagtcatt
3600 tcatcccaca tattccttgg atttctctcc aatgaatgac attctatctt
gcaaattcaa 3660 caattataat aagatatacc aaagtagcgg tatagtggca
atcaaaaagc ttctctggtg 3720 tgcttctcgt atttattttt attctaatga
tccattaaag gtatatattt atttcttgtt 3780 atataatcct tttgtttatt
acatgggctg gatacataaa ggtattttga tttaattttt 3840 tgcttaaatt
caatcccccc tcgttcagtg tcaactgtaa tggtaggaaa ttaccatact 3900
tttgaagaag caaaaaaaat gaaagaaaaa aaaaatcgta tttccaggtt agacgttccg
3960 cagaatctag aatgcggtat gcggtacatt gttcttcgaa cgtaaaagtt
gcgctccctg 4020 agatattgta catttttgct tttacaagta caagtacatc
gtacaactat gtactactgt 4080 tgatgcatcc acaacagttt gttttgtttt
tttttgtttt ttttttttct aatgattcat 4140 taccgctatg tatacctact
tgtacttgta gtaagccggg ttattggcgt tcaattaatc 4200 atagacttat
gaatctgcac ggtgtgcgct gcgagttact tttagcttat gcatgctact 4260
tgggtgtaat attgggatct gttcggaaat caacggatgc tcaaccgatt tcgacagtaa
4320 ttaattaagt catacacaag tcagctttct tcgagcctca tataagtata
agtagttcaa 4380 cgtattagca ctgtacccag catctccgta tcgagaaaca
caacaacatg ccccattgga 4440 cagatcatgc ggatacacag gttgtgcagt
atcatacata ctcgatcaga caggtcgtct 4500 gaccatcata caagctgaac
aagcgctcca tacttgcacg ctctctatat acacagttaa 4560 attacatatc
catagtctaa cctctaacag ttaatcttct ggtaagcctc ccagccagcc 4620
ttctggtatc gcttggcctc ctcaatagga tctcggttct ggccgtacag acctcggccg
4680 acaattatga tatccgttcc ggtagacatg acatcctcaa cagttcggta
ctgctgtccg 4740 agagcgtctc ccttgtcgtc aagacccacc ccgggggtca
gaataagcca gtcctcagag 4800 tcgcccttag gtcggttctg ggcaatgaag
ccaaccacaa actcggggtc ggatcgggca 4860 agctcaatgg tctgcttgga
gtactcgcca gtggccagag agcccttgca agacagctcg 4920 gccagcatga
gcagacctct ggccagcttc tcgttgggag aggggactag gaactccttg 4980
tactgggagt tctcgtagtc agagacgtcc tccttcttct gttcagagac agtttcctcg
5040 gcaccagctc gcaggccagc aatgattccg gttccgggta caccgtgggc
gttggtgata 5100 tcggaccact cggcgattcg gtgacaccgg tactggtgct
tgacagtgtt gccaatatct 5160 gcgaactttc tgtcctcgaa caggaagaaa
ccgtgcttaa gagcaagttc cttgaggggg 5220 agcacagtgc cggcgtaggt
gaagtcgtca atgatgtcga tatgggtttt gatcatgcac 5280 acataaggtc
cgaccttatc ggcaagctca atgagctcct tggtggtggt aacatccaga 5340
gaagcacaca ggttggtttt cttggctgcc acgagcttga gcactcgagc ggcaaaggcg
5400 gacttgtgga cgttagctcg agcttcgtag gagggcattt tggtggtgaa
gaggagactg 5460 aaataaattt agtctgcaga actttttatc ggaaccttat
ctggggcagt gaagtatatg 5520 ttatggtaat agttacgagt tagttgaact
tatagataga ctggactata cggctatcgg 5580 tccaaattag aaagaacgtc
aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga 5640 tcatgatgaa
agccagcaat gacgttgcag ctgatattgt tgtcggccaa ccgcgccgaa 5700
aacgcagctg tcagacccac agcctccaac gaagaatgta tcgtcaaagt gatccaagca
5760 cactcatagt tggagtcgta ctccaaaggc ggcaatgacg agtcagacag
atactcgtcg 5820 actcaggcga cgacggaatt cctgcagccc atctgcagaa
ttcaggagag accgggttgg 5880 cggcgtattt gtgtcccaaa aaacagcccc
aattgcccca attgacccca aattgaccca 5940 gtagcgggcc caaccccggc
gagagccccc ttcaccccac atatcaaacc tcccccggtt 6000 cccacacttg
ccgttaaggg cgtagggtac tgcagtctgg aatctacgct tgttcagact 6060
ttgtactagt ttctttgtct ggccatccgg gtaacccatg ccggacgcaa aatagactac
6120 tgaaaatttt tttgctttgt ggttgggact ttagccaagg gtataaaaga
ccaccgtccc 6180 cgaattacct ttcctcttct tttctctctc tccttgtcaa
ctcacacccg aaatcgttaa 6240 gcatttcctt ctgagtataa gaatcattca
ccatggatcc actagttcta gagcggccgc 6300 caccgcggcc cgagattccg
gcctcttcgg ccgccaagcg acccgggtgg acgtctagag 6360 gtacctagca
attaacagat agtttgccgg tgataattct cttaacctcc cacactcctt 6420
tgacataacg atttatgtaa cgaaactgaa atttgaccag atattgtgtc cgc 6473
<210> SEQ ID NO 70 <211> LENGTH: 6970 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY5-22GPD <400>
SEQUENCE: 70 tcgacgcagt aggatgtcct gcacgggtct ttttgtgggg tgtggagaaa
ggggtgcttg 60 gagatggaag ccggtagaac cgggctgctt gtgcttggag
atggaagccg gtagaaccgg 120 gctgcttggg gggatttggg gccgctgggc
tccaaagagg ggtaggcatt tcgttggggt 180 tacgtaattg cggcatttgg
gtcctgcgcg catgtcccat tggtcagaat tagtccggat 240 aggagactta
tcagccaatc acagcgccgg atccacctgt aggttgggtt gggtgggagc 300
acccctccac agagtagagt caaacagcag cagcaacatg atagttgggg gtgtgcgtgt
360 taaaggaaaa aaaagaagct tgggttatat tcccgctcta tttagaggtt
gcgggataga 420 cgccgacgga gggcaatggc gccatggaac cttgcggata
tcgatacgcc gcggcggact 480 gcgtccgaac cagctccagc agcgtttttt
ccgggccatt gagccgactg cgaccccgcc 540 aacgtgtctt ggcccacgca
ctcatgtcat gttggtgttg ggaggccact ttttaagtag 600 cacaaggcac
ctagctcgca gcaaggtgtc cgaaccaaag aagcggctgc agtggtgcaa 660
acggggcgga aacggcggga aaaagccacg ggggcacgaa ttgaggcacg ccctcgaatt
720 tgagacgagt cacggcccca ttcgcccgcg caatggctcg ccaacgcccg
gtcttttgca 780 ccacatcagg ttaccccaag ccaaaccttt gtgttaaaaa
gcttaacata ttataccgaa 840 cgtaggtttg ggcgggcttg ctccgtctgt
ccaaggcaac atttatataa gggtctgcat 900 cgccggctca attgaatctt
ttttcttctt ctcttctcta tattcattct tgaattaaac 960 acacatcaat
ccgcggccgc caccgcggcc cgagattccg gcctcttcgg ccgccaagcg 1020
acccgggtgg acgtctagag gtacctagca attaacagat agtttgccgg tgataattct
1080 cttaacctcc cacactcctt tgacataacg atttatgtaa cgaaactgaa
atttgaccag 1140 atattgtgtc cgcggtggag ctccagcttt tgttcccttt
agtgagggtt aatttcgagc 1200 ttggcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca 1260 cacaacgtac gagccggaag
cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 1320 ctcacattaa
ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 1380
ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
1440 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
ggtatcagct 1500 cactcaaagg cggtaatacg gttatccaca gaatcagggg
ataacgcagg aaagaacatg 1560 tgagcaaaag gccagcaaaa ggccaggaac
cgtaaaaagg ccgcgttgct ggcgtttttc 1620 cataggctcc gcccccctga
cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 1680 aacccgacag
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 1740
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
1800 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
tcgctccaag 1860 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
gcgccttatc cggtaactat 1920 cgtcttgagt ccaacccggt aagacacgac
ttatcgccac tggcagcagc cactggtaac 1980 aggattagca gagcgaggta
tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 2040 tacggctaca
ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 2100
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
2160 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
tcctttgatc 2220 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
gttaagggat tttggtcatg 2280 agattatcaa aaaggatctt cacctagatc
cttttaaatt aaaaatgaag ttttaaatca 2340 atctaaagta tatatgagta
aacttggtct gacagttacc aatgcttaat cagtgaggca 2400 cctatctcag
cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 2460
ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
2520 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
ggccgagcgc 2580 agaagtggtc ctgcaacttt atccgcctcc atccagtcta
ttaattgttg ccgggaagct 2640 agagtaagta gttcgccagt taatagtttg
cgcaacgttg ttgccattgc tacaggcatc 2700 gtggtgtcac gctcgtcgtt
tggtatggct tcattcagct ccggttccca acgatcaagg 2760 cgagttacat
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 2820
gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
2880 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
ctcaaccaag 2940 tcattctgag aatagtgtat gcggcgaccg agttgctctt
gcccggcgtc aatacgggat 3000 aataccgcgc cacatagcag aactttaaaa
gtgctcatca ttggaaaacg ttcttcgggg 3060 cgaaaactct caaggatctt
accgctgttg agatccagtt cgatgtaacc cactcgtgca 3120 cccaactgat
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 3180
aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
3240 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
cggatacata 3300 tttgaatgta tttagaaaaa taaacaaata ggggttccgc
gcacatttcc ccgaaaagtg 3360 ccacctgacg cgccctgtag cggcgcatta
agcgcggcgg gtgtggtggt tacgcgcagc 3420 gtgaccgcta cacttgccag
cgccctagcg cccgctcctt tcgctttctt cccttccttt 3480 ctcgccacgt
tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 3540
cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
3600 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
cacgttcttt 3660 aatagtggac tcttgttcca aactggaaca acactcaacc
ctatctcggt ctattctttt 3720 gatttataag ggattttgcc gatttcggcc
tattggttaa aaaatgagct gatttaacaa 3780 aaatttaacg cgaattttaa
caaaatatta acgcttacaa tttccattcg ccattcaggc 3840 tgcgcaactg
ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 3900
aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
3960 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
ttgggtaccg 4020 ggccccccct cgaggtcgat ggtgtcgata agcttgatat
cgaattcatg tcacacaaac 4080 cgatcttcgc ctcaaggaaa cctaattcta
catccgagag actgccgaga tccagtctac 4140 actgattaat tttcgggcca
ataatttaaa aaaatcgtgt tatataatat tatatgtatt 4200 atatatatac
atcatgatga tactgacagt catgtcccat tgctaaatag acagactcca 4260
tctgccgcct ccaactgatg ttctcaatat ttaaggggtc atctcgcatt gtttaataat
4320 aaacagactc catctaccgc ctccaaatga tgttctcaaa atatattgta
tgaacttatt 4380 tttattactt agtattatta gacaacttac ttgctttatg
aaaaacactt cctatttagg 4440 aaacaattta taatggcagt tcgttcattt
aacaatttat gtagaataaa tgttataaat 4500 gcgtatggga aatcttaaat
atggatagca taaatgatat ctgcattgcc taattcgaaa 4560 tcaacagcaa
cgaaaaaaat cccttgtaca acataaatag tcatcgagaa atatcaacta 4620
tcaaagaaca gctattcaca cgttactatt gagattatta ttggacgaga atcacacact
4680 caactgtctt tctctcttct agaaatacag gtacaagtat gtactattct
cattgttcat 4740 acttctagtc atttcatccc acatattcct tggatttctc
tccaatgaat gacattctat 4800 cttgcaaatt caacaattat aataagatat
accaaagtag cggtatagtg gcaatcaaaa 4860 agcttctctg gtgtgcttct
cgtatttatt tttattctaa tgatccatta aaggtatata 4920 tttatttctt
gttatataat ccttttgttt attacatggg ctggatacat aaaggtattt 4980
tgatttaatt ttttgcttaa attcaatccc ccctcgttca gtgtcaactg taatggtagg
5040 aaattaccat acttttgaag aagcaaaaaa aatgaaagaa aaaaaaaatc
gtatttccag 5100 gttagacgtt ccgcagaatc tagaatgcgg tatgcggtac
attgttcttc gaacgtaaaa 5160 gttgcgctcc ctgagatatt gtacattttt
gcttttacaa gtacaagtac atcgtacaac 5220 tatgtactac tgttgatgca
tccacaacag tttgttttgt ttttttttgt tttttttttt 5280 tctaatgatt
cattaccgct atgtatacct acttgtactt gtagtaagcc gggttattgg 5340
cgttcaatta atcatagact tatgaatctg cacggtgtgc gctgcgagtt acttttagct
5400 tatgcatgct acttgggtgt aatattggga tctgttcgga aatcaacgga
tgctcaaccg 5460 atttcgacag taattaatta agtcatacac aagtcagctt
tcttcgagcc tcatataagt 5520 ataagtagtt caacgtatta gcactgtacc
cagcatctcc gtatcgagaa acacaacaac 5580 atgccccatt ggacagatca
tgcggataca caggttgtgc agtatcatac atactcgatc 5640 agacaggtcg
tctgaccatc atacaagctg aacaagcgct ccatacttgc acgctctcta 5700
tatacacagt taaattacat atccatagtc taacctctaa cagttaatct tctggtaagc
5760 ctcccagcca gccttctggt atcgcttggc ctcctcaata ggatctcggt
tctggccgta 5820 cagacctcgg ccgacaatta tgatatccgt tccggtagac
atgacatcct caacagttcg 5880 gtactgctgt ccgagagcgt ctcccttgtc
gtcaagaccc accccggggg tcagaataag 5940 ccagtcctca gagtcgccct
taggtcggtt ctgggcaatg aagccaacca caaactcggg 6000 gtcggatcgg
gcaagctcaa tggtctgctt ggagtactcg ccagtggcca gagagccctt 6060
gcaagacagc tcggccagca tgagcagacc tctggccagc ttctcgttgg gagaggggac
6120 taggaactcc ttgtactggg agttctcgta gtcagagacg tcctccttct
tctgttcaga 6180 gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt
ccggttccgg gtacaccgtg 6240 ggcgttggtg atatcggacc actcggcgat
tcggtgacac cggtactggt gcttgacagt 6300 gttgccaata tctgcgaact
ttctgtcctc gaacaggaag aaaccgtgct taagagcaag 6360 ttccttgagg
gggagcacag tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt 6420
tttgatcatg cacacataag gtccgacctt atcggcaagc tcaatgagct ccttggtggt
6480 ggtaacatcc agagaagcac acaggttggt tttcttggct gccacgagct
tgagcactcg 6540 agcggcaaag gcggacttgt ggacgttagc tcgagcttcg
taggagggca ttttggtggt 6600 gaagaggaga ctgaaataaa tttagtctgc
agaacttttt atcggaacct tatctggggc 6660 agtgaagtat atgttatggt
aatagttacg agttagttga acttatagat agactggact 6720 atacggctat
cggtccaaat tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc 6780
gacaaaaatg tgatcatgat gaaagccagc aatgacgttg cagctgatat tgttgtcggc
6840 caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc aacgaagaat
gtatcgtcaa 6900 agtgatccaa gcacactcat agttggagtc gtactccaaa
ggcggcaatg acgagtcaga 6960 cagatactcg 6970 <210> SEQ ID NO 71
<211> LENGTH: 968 <212> TYPE: DNA <213> ORGANISM:
Yarrowia lipolytica <300> PUBLICATION INFORMATION:
<302> TITLE: GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND
PHOSPHOGLYCERATE MUTASE PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS
YEAST <310> PATENT DOCUMENT NUMBER: US-2005-0014270-A1
<311> PATENT FILING DATE: 2004-06-16 <312> PUBLICATION
DATE: 2005-01-20 <313> RELEVANT RESIDUES IN SEQ ID NO:
(1)..(968) <400> SEQUENCE: 71 tcgacgcagt aggatgtcct
gcacgggtct ttttgtgggg tgtggagaaa ggggtgcttg 60 gagatggaag
ccggtagaac cgggctgctt gtgcttggag atggaagccg gtagaaccgg 120
gctgcttggg gggatttggg gccgctgggc tccaaagagg ggtaggcatt tcgttggggt
180 tacgtaattg cggcatttgg gtcctgcgcg catgtcccat tggtcagaat
tagtccggat 240 aggagactta tcagccaatc acagcgccgg atccacctgt
aggttgggtt gggtgggagc 300 acccctccac agagtagagt caaacagcag
cagcaacatg atagttgggg gtgtgcgtgt 360 taaaggaaaa aaaagaagct
tgggttatat tcccgctcta tttagaggtt gcgggataga 420 cgccgacgga
gggcaatggc gccatggaac cttgcggata tcgatacgcc gcggcggact 480
gcgtccgaac cagctccagc agcgtttttt ccgggccatt gagccgactg cgaccccgcc
540 aacgtgtctt ggcccacgca ctcatgtcat gttggtgttg ggaggccact
ttttaagtag 600 cacaaggcac ctagctcgca gcaaggtgtc cgaaccaaag
aagcggctgc agtggtgcaa 660 acggggcgga aacggcggga aaaagccacg
ggggcacgaa ttgaggcacg ccctcgaatt 720 tgagacgagt cacggcccca
ttcgcccgcg caatggctcg ccaacgcccg gtcttttgca 780 ccacatcagg
ttaccccaag ccaaaccttt gtgttaaaaa gcttaacata ttataccgaa 840
cgtaggtttg ggcgggcttg ctccgtctgt ccaaggcaac atttatataa gggtctgcat
900 cgccggctca attgaatctt ttttcttctt ctcttctcta tattcattct
tgaattaaac 960 acacatca 968 <210> SEQ ID NO 72 <211>
LENGTH: 8630 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pYZDE2-S <400> SEQUENCE: 72 ggtggagctc cagcttttgt
tccctttagt gagggttaat ttcgagcttg gcgtaatcat 60 ggtcatagct
gtttcctgtg tgaaattgtt atccgctcac aattccacac aacgtacgag 120
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
180 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
cattaatgaa 240 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
ctcttccgct tcctcgctca 300 ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg 360 taatacggtt atccacagaa
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 420 agcaaaaggc
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 480
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
540 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc 600 tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg ctttctcata 660 gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc 720 acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca 780 acccggtaag
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 840
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
900 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
aaaagagttg 960 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
tggttttttt gtttgcaagc 1020 agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt 1080 ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 1140 ggatcttcac
ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 1200
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
1260 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
actacgatac 1320 gggagggctt accatctggc cccagtgctg caatgatacc
gcgagaccca cgctcaccgg 1380 ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg 1440 caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt 1500 cgccagttaa
tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 1560
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
1620 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta 1680 agttggccgc agtgttatca ctcatggtta tggcagcact
gcataattct cttactgtca 1740 tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat 1800 agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat acgggataat accgcgccac 1860 atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 1920
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
1980 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg 2040 caaaaaaggg aataagggcg acacggaaat gttgaatact
catactcttc ctttttcaat 2100 attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt 2160 agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgcgc 2220 cctgtagcgg
cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2280
ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2340 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
tttagtgctt 2400 tacggcacct cgaccccaaa aaacttgatt agggtgatgg
ttcacgtagt gggccatcgc 2460 cctgatagac ggtttttcgc cctttgacgt
tggagtccac gttctttaat agtggactct 2520 tgttccaaac tggaacaaca
ctcaacccta tctcggtcta ttcttttgat ttataaggga 2580 ttttgccgat
ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 2640
attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
2700 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
ggggatgtgc 2760 tgcaaggcga ttaagttggg taacgccagg gttttcccag
tcacgacgtt gtaaaacgac 2820 ggccagtgaa ttgtaatacg actcactata
gggcgaattg ggtaccgggc cccccctcga 2880 ggtcgatggt gtcgataagc
ttgatatcga attcatgtca cacaaaccga tcttcgcctc 2940 aaggaaacct
aattctacat ccgagagact gccgagatcc agtctacact gattaatttt 3000
cgggccaata atttaaaaaa atcgtgttat ataatattat atgtattata tatatacatc
3060 atgatgatac tgacagtcat gtcccattgc taaatagaca gactccatct
gccgcctcca 3120 actgatgttc tcaatattta aggggtcatc tcgcattgtt
taataataaa cagactccat 3180 ctaccgcctc caaatgatgt tctcaaaata
tattgtatga acttattttt attacttagt 3240 attattagac aacttacttg
ctttatgaaa aacacttcct atttaggaaa caatttataa 3300 tggcagttcg
ttcatttaac aatttatgta gaataaatgt tataaatgcg tatgggaaat 3360
cttaaatatg gatagcataa atgatatctg cattgcctaa ttcgaaatca acagcaacga
3420 aaaaaatccc ttgtacaaca taaatagtca tcgagaaata tcaactatca
aagaacagct 3480 attcacacgt tactattgag attattattg gacgagaatc
acacactcaa ctgtctttct 3540 ctcttctaga aatacaggta caagtatgta
ctattctcat tgttcatact tctagtcatt 3600 tcatcccaca tattccttgg
atttctctcc aatgaatgac attctatctt gcaaattcaa 3660 caattataat
aagatatacc aaagtagcgg tatagtggca atcaaaaagc ttctctggtg 3720
tgcttctcgt atttattttt attctaatga tccattaaag gtatatattt atttcttgtt
3780 atataatcct tttgtttatt acatgggctg gatacataaa ggtattttga
tttaattttt 3840 tgcttaaatt caatcccccc tcgttcagtg tcaactgtaa
tggtaggaaa ttaccatact 3900 tttgaagaag caaaaaaaat gaaagaaaaa
aaaaatcgta tttccaggtt agacgttccg 3960 cagaatctag aatgcggtat
gcggtacatt gttcttcgaa cgtaaaagtt gcgctccctg 4020 agatattgta
catttttgct tttacaagta caagtacatc gtacaactat gtactactgt 4080
tgatgcatcc acaacagttt gttttgtttt tttttgtttt ttttttttct aatgattcat
4140 taccgctatg tatacctact tgtacttgta gtaagccggg ttattggcgt
tcaattaatc 4200 atagacttat gaatctgcac ggtgtgcgct gcgagttact
tttagcttat gcatgctact 4260 tgggtgtaat attgggatct gttcggaaat
caacggatgc tcaaccgatt tcgacagtaa 4320 taatttgaat cgaatcggag
cctaaaatga acccgagtat atctcataaa attctcggtg 4380 agaggtctgt
gactgtcagt acaaggtgcc ttcattatgc cctcaacctt accatacctc 4440
actgaatgta gtgtacctct aaaaatgaaa tacagtgcca aaagccaagg cactgagctc
4500 gtctaacgga cttgatatac aaccaattaa aacaaatgaa aagaaataca
gttctttgta 4560 tcatttgtaa caattaccct gtacaaacta aggtattgaa
atcccacaat attcccaaag 4620 tccacccctt tccaaattgt catgcctaca
actcatatac caagcactaa cctaccaaac 4680 accactaaaa ccccacaaaa
tatatcttac cgaatataca gtaacaagct accaccacac 4740 tcgttgggtg
cagtcgccag cttaaagata tctatccaca tcagccacaa ctcccttcct 4800
ttaataaacc gactacaccc ttggctattg aggttatgag tgaatatact gtagacaaga
4860 cactttcaag aagactgttt ccaaaacgta ccactgtcct ccactacaaa
cacacccaat 4920 ctgcttcttc tagtcaaggt tgctacaccg gtaaattata
aatcatcatt tcattagcag 4980 ggcagggccc tttttataga gtcttataca
ctagcggacc ctgccggtag accaacccgc 5040 aggcgcgtca gtttgctcct
tccatcaatg cgtcgtagaa acgacttact ccttcttgag 5100 cagctccttg
accttgttgg caacaagtct ccgacctcgg aggtggagga agagcctccg 5160
atatcggcgg tagtgatacc agcctcgacg gactccttga cggcagcctc aacagcgtca
5220 ccggcgggct tcatgttaag agagaacttg agcatcatgg cggcagacag
aatggtggca 5280 atggggttga ccttctgctt gccgagatcg ggggcagatc
cgtgacaggg ctcgtacaga 5340 ccgaacgcct cgttggtgtc gggcagagaa
gccagagagg cggagggcag cagacccaga 5400 gaaccgggga tgacggaggc
ctcgtcggag atgatatcgc caaacatgtt ggtggtgatg 5460 atgataccat
tcatcttgga gggctgcttg atgaggatca tggcggccga gtcgatcagc 5520
tggtggttga gctcgagctg ggggaattcg tccttgagga ctcgagtgac agtctttcgc
5580 caaagtcgag aggaggccag cacgttggcc ttgtcaagag accacacggg
aagagggggg 5640 ttgtgctgaa gggccaggaa ggcggccatt cgggcaattc
gctcaacctc aggaacggag 5700 taggtctcgg tgtcggaagc gacgccagat
ccgtcatcct cctttcgctc tccaaagtag 5760 atacctccga cgagctctcg
gacaatgatg aagtcggtgc cctcaacgtt tcggatgggg 5820 gagagatcgg
cgagcttggg cgacagcagc tggcagggtc gcaggttggc gtacaggttc 5880
aggtcctttc gcagcttgag gagaccctgc tcgggtcgca cgtcggttcg tccgtcggga
5940 gtggtccata cggtgttggc agcgcctccg acagcaccga gcataataga
gtcagccttt 6000 cggcagatgt cgagagtagc gtcggtgatg ggctcgccct
ccttctcaat ggcagctcct 6060 ccaatgagtc ggtcctcaaa cacaaactcg
gtgccggagg cctcagcaac agacttgagc 6120 accttgacgg cctcggcaat
cacctcgggg ccacagaagt cgccgccgag aagaacaatc 6180 ttcttggagt
cagtcttggt cttcttagtt tcgggttcca ttgtggatgt gtgtggttgt 6240
atgtgtgatg tggtgtgtgg agtgaaaatc tgtggctggc aaacgctctt gtatatatac
6300 gcacttttgc ccgtgctatg tggaagacta aacctccgaa gattgtgact
caggtagtgc 6360 ggtatcggct agggacccaa accttgtcga tgccgatagc
gctatcgaac gtaccccagc 6420 cggccgggag tatgtcggag gggacatacg
agatcgtcaa gggtttgtgg ccaactggta 6480 aatttaaatg atgtcgacgc
agtaggatgt cctgcacggg tctttttgtg gggtgtggag 6540 aaaggggtgc
ttggagatgg aagccggtag aaccgggctg cttgtgcttg gagatggaag 6600
ccggtagaac cgggctgctt ggggggattt ggggccgctg ggctccaaag aggggtaggc
6660 atttcgttgg ggttacgtaa ttgcggcatt tgggtcctgc gcgcatgtcc
cattggtcag 6720 aattagtccg gataggagac ttatcagcca atcacagcgc
cggatccacc tgtaggttgg 6780 gttgggtggg agcacccctc cacagagtag
agtcaaacag cagcagcaac atgatagttg 6840 ggggtgtgcg tgttaaagga
aaaaaaagaa gcttgggtta tattcccgct ctatttagag 6900 gttgcgggat
agacgccgac ggagggcaat ggcgccatgg aaccttgcgg atatcgatac 6960
gccgcggcgg actgcgtccg aaccagctcc agcagcgttt tttccgggcc attgagccga
7020 ctgcgacccc gccaacgtgt cttggcccac gcactcatgt catgttggtg
ttgggaggcc 7080 actttttaag tagcacaagg cacctagctc gcagcaaggt
gtccgaacca aagaagcggc 7140 tgcagtggtg caaacggggc ggaaacggcg
ggaaaaagcc acgggggcac gaattgaggc 7200 acgccctcga atttgagacg
agtcacggcc ccattcgccc gcgcaatggc tcgccaacgc 7260 ccggtctttt
gcaccacatc aggttacccc aagccaaacc tttgtgttaa aaagcttaac 7320
atattatacc gaacgtaggt ttgggcgggc ttgctccgtc tgtccaaggc aacatttata
7380 taagggtctg catcgccggc tcaattgaat cttttttctt cttctcttct
ctatattcat 7440 tcttgaatta aacacacatc aatccatggc aaacagcagc
gtgtgggatg atgtggtggg 7500 ccgcgtggag accggcgtgg accagtggat
ggatggcgcc aagccgtacg cactcaccga 7560 tgggctcccg atgatggacg
tgtccaccat gctggcattc gaggtgggat acatggccat 7620 gctgctcttc
ggcatcccga tcatgaagca gatggagaag ccttttgagc tcaagaccat 7680
caagctcttg cacaacttgt ttctcttcgg actttccttg tacatgtgcg tggagaccat
7740 ccgccaggct atcctcggag gctacaaagt gtttggaaac gacatggaga
agggcaacga 7800 gtctcatgct cagggcatgt ctcgcatcgt gtacgtgttc
tacgtgtcca aggcatacga 7860 gttcttggat accgccatca tgatcctttg
caagaagttc aaccaggttt ccttcttgca 7920 tgtgtaccac catgccactc
atttttgcca tctggtgggc tatccgccaa gtacgctcca 7980 ggaggtgatg
cgtacttttt cagtgatcct caactctttc gtgcacaccg tcatgtacgg 8040
catactactt cttctcctcc caagggttcg ggttcgtgaa gccaatcaag ccgtacatca
8100 ccacccttca gatgacccag ttcatggcaa tgcttgtgca gtccttgtac
gactacctct 8160 tcccatgcga ctacccacag gctcttgtgc agctccttgg
agtgtacatg atcaccttgc 8220 ttgccctctt cggcaacttt tttgtgcaga
gctatcttaa aaagccaaaa aagagcaaga 8280 ccaactaaaa ctgcctgcat
gatatgccgc tcgccggcgt tcgaattgac tcagaaagcg 8340 agttaaggcg
acacgcaaac tctatatttt ttcaaacgtg ttgccgtcac tcattcgcca 8400
tctgtttact acgtgtctgt tcaatgagca tgttcttgaa tctaaagaat ctcgaatgtt
8460 ttttaaaaaa agaattcgat atcaagctta cgcgtcgacc cgggtggacg
tctagaggta 8520 cctagcaatt aacagatagt ttgccggtga taattctctt
aacctcccac actcctttga 8580 cataacgatt tatgtaacga aactgaaatt
tgaccagata ttgtgtccgc 8630 <210> SEQ ID NO 73 <211>
LENGTH: 18 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
GPDsense <400> SEQUENCE: 73 atacgagatc gtcaaggg 18
<210> SEQ ID NO 74 <211> LENGTH: 26 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer GPDantisense <400>
SEQUENCE: 74 gcggccgcgg attgatgtgt gtttaa 26 <210> SEQ ID NO
75 <211> LENGTH: 6339 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR136 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (4078)..(4078)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 75 ggccgcgaca caagtgtgag agtactaaat aaatgctttg gttgtacgaa
atcattacac 60 taaataaaat aatcaaagct tatatatgcc ttccgctaag
gccgaatgca aagaaattgg 120 ttctttctcg ttatcttttg ccacttttac
tagtacgtat taattactac ttaatcatct 180 ttgtttacgg ctcattatat
ccgtacgtcg agtcgacctg caggcatgca agcttggcgt 240 aatcatggtc
atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 300
tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
360 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
cagctgcatt 420 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
tgggcgctct tccgcttcct 480 cgctcactga ctcgctgcgc tcggtcgttc
ggctgcggcg agcggtatca gctcactcaa 540 aggcggtaat acggttatcc
acagaatcag gggataacgc aggaaagaac atgtgagcaa 600 aaggccagca
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 660
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
720 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
tctcctgttc 780 cgaccctgcc gcttaccgga tacctgtccg cctttctccc
ttcgggaagc gtggcgcttt 840 ctcatagctc acgctgtagg tatctcagtt
cggtgtaggt cgttcgctcc aagctgggct 900 gtgtgcacga accccccgtt
cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 960 agtccaaccc
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1020
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
1080 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
ttcggaaaaa 1140 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
tagcggtggt ttttttgttt 1200 gcaagcagca gattacgcgc agaaaaaaag
gatctcaaga agatcctttg atcttttcta 1260 cggggtctga cgctcagtgg
aacgaaaact cacgttaagg gattttggtc atgagattat 1320 caaaaaggat
cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1380
gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
1440 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
tagataacta 1500 cgatacggga gggcttacca tctggcccca gtgctgcaat
gataccgcga gacccacgct 1560 caccggctcc agatttatca gcaataaacc
agccagccgg aagggccgag cgcagaagtg 1620 gtcctgcaac tttatccgcc
tccatccagt ctattaattg ttgccgggaa gctagagtaa 1680 gtagttcgcc
agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 1740
cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
1800 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
atcgttgtca 1860 gaagtaagtt ggccgcagtg ttatcactca tggttatggc
agcactgcat aattctctta 1920 ctgtcatgcc atccgtaaga tgcttttctg
tgactggtga gtactcaacc aagtcattct 1980 gagaatagtg tatgcggcga
ccgagttgct cttgcccggc gtcaatacgg gataataccg 2040 cgccacatag
cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2100
tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
2160 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
ggaaggcaaa 2220 atgccgcaaa aaagggaata agggcgacac ggaaatgttg
aatactcata ctcttccttt 2280 ttcaatatta ttgaagcatt tatcagggtt
attgtctcat gagcggatac atatttgaat 2340 gtatttagaa aaataaacaa
ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 2400 acgtctaaga
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 2460
cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
2520 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
cagggcgcgt 2580 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
ggcatcagag cagattgtac 2640 tgagagtgca ccatatgcgg tgtgaaatac
cgcacagatg cgtaaggaga aaataccgca 2700 tcaggcgcca ttcgccattc
aggctgcgca actgttggga agggcgatcg gtgcgggcct 2760 cttcgctatt
acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 2820
cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
2880 cccggggatc ctctagacgt acgtcctcga agagaagggt taataacaca
ttttttaaca 2940 tttttaacac aaattttagt tatttaaaaa tttattaaaa
aatttaaaat aagaagagga 3000 actctttaaa taaatctaac ttacaaaatt
tatgattttt aataagtttt caccaataaa 3060 aaatgtcata aaaatatgtt
aaaaagtata ttatcaatat tctctttatg ataaataaaa 3120 agaaaaaaaa
aataaaagtt aagtgaaaat gagattgaag tgactttagg tgtgtataaa 3180
tatatcaacc ccgccaacaa tttatttaat ccaaatatat tgaagtatat tattccatag
3240 cctttattta tttatatatt tattatataa aagctttatt tgttctaggt
tgttcatgaa 3300 atattttttt ggttttatct ccgttgtaag aaaatcatgt
gctttgtgtc gccactcact 3360 attgcagctt tttcatgcat tggtcagatt
gacggttgat tgtatttttg ttttttatgg 3420 ttttgtgtta tgacttaagt
cttcatctct ttatctcttc atcaggtttg atggttacct 3480 aatatggtcc
atgggtacat gcatggttaa attaggtggc caactttgtt gtgaacgata 3540
gaattttttt tatattaagt aaactatttt tatattatga aataataata aaaaaaatat
3600 tttatcatta ttaacaaaat catattagtt aatttgttaa ctctataata
aaagaaatac 3660 tgtaacattc acattacatg gtaacatctt tccacccttt
catttgtttt ttgtttgatg 3720 actttttttc ttgtttaaat ttatttccct
tcttttaaat ttggaataca ttatcatcat 3780 atataaacta aaatactaaa
aacaggatta cacaaatgat aaataataac acaaatattt 3840 ataaatctag
ctgcaatata tttaaactag ctatatcgat attgtaaaat aaaactagct 3900
gcattgatac tgataaaaaa atatcatgtg ctttctggac tgatgatgca gtatactttt
3960 gacattgcct ttattttatt tttcagaaaa gctttcttag ttctgggttc
ttcattattt 4020 gtttcccatc tccattgtga attgaatcat ttgcttcgtg
tcacaaatac aatttagnta 4080 ggtacatgca ttggtcagat tcacggttta
ttatgtcatg acttaagttc atggtagtac 4140 attacctgcc acgcatgcat
tatattggtt agatttgata ggcaaatttg gttgtcaaca 4200 atataaatat
aaataatgtt tttatattac gaaataacag tgatcaaaac aaacagtttt 4260
atctttatta acaagatttt gtttttgttt gatgacgttt tttaatgttt acgctttccc
4320 ccttcttttg aatttagaac actttatcat cataaaatca aatactaaaa
aaattacata 4380 tttcataaat aataacacaa atatttttaa aaaatctgaa
ataataatga acaatattac 4440 atattatcac gaaaattcat taataaaaat
attatataaa taaaatgtaa tagtagttat 4500 atgtaggaaa aaagtactgc
acgcataata tatacaaaaa gattaaaatg aactattata 4560 aataataaca
ctaaattaat ggtgaatcat atcaaaataa tgaaaaagta aataaaattt 4620
gtaattaact tctatatgta ttacacacac aaataataaa taatagtaaa aaaaattatg
4680 ataaatattt accatctcat aagatattta aaataatgat aaaaatatag
attatttttt 4740 atgcaactag ctagccaaaa agagaacacg ggtatatata
aaaagagtac ctttaaattc 4800 tactgtactt cctttattcc tgacgttttt
atatcaagtg gacatacgtg aagattttaa 4860 ttatcagtct aaatatttca
ttagcactta atacttttct gttttattcc tatcctataa 4920 gtagtcccga
ttctcccaac attgcttatt cacacaacta actaagaaag tcttccatag 4980
ccccccaagc ggccgcatgg gaacggacca aggaaaaacc ttcacctggg aagagctggc
5040 ggcccataac accaaggacg acctactctt ggccatccgc ggcagggtgt
acgatgtcac 5100 aaagttcttg agccgccatc ctggtggagt ggacactctc
ctgctcggag ctggccgaga 5160 tgttactccg gtctttgaga tgtatcacgc
gtttggggct gcagatgcca ttatgaagaa 5220 gtactatgtc ggtacactgg
tctcgaatga gctgcccatc ttcccggagc caacggtgtt 5280 ccacaaaacc
atcaagacga gagtcgaggg ctactttacg gatcggaaca ttgatcccaa 5340
gaatagacca gagatctggg gacgatacgc tcttatcttt ggatccttga tcgcttccta
5400 ctacgcgcag ctctttgtgc ctttcgttgt cgaacgcaca tggcttcagg
tggtgtttgc 5460 aatcatcatg ggatttgcgt gcgcacaagt cggactcaac
cctcttcatg atgcgtctca 5520 cttttcagtg acccacaacc ccactgtctg
gaagattctg ggagccacgc acgacttttt 5580 caacggagca tcgtacctgg
tgtggatgta ccaacatatg ctcggccatc acccctacac 5640 caacattgct
ggagcagatc ccgacgtgtc gacgtctgag cccgatgttc gtcgtatcaa 5700
gcccaaccaa aagtggtttg tcaaccacat caaccagcac atgtttgttc ctttcctgta
5760 cggactgctg gcgttcaagg tgcgcattca ggacatcaac attttgtact
ttgtcaagac 5820 caatgacgct attcgtgtca atcccatctc gacatggcac
actgtgatgt tctggggcgg 5880 caaggctttc tttgtctggt atcgcctgat
tgttcccctg cagtatctgc ccctgggcaa 5940 ggtgctgctc ttgttcacgg
tcgcggacat ggtgtcgtct tactggctgg cgctgacctt 6000 ccaggcgaac
cacgttgttg aggaagttca gtggccgttg cctgacgaga acgggatcat 6060
ccaaaaggac tgggcagcta tgcaggtcga gactacgcag gattacgcac acgattcgca
6120 cctctggacc agcatcactg gcagcttgaa ctaccaggct gtgcaccatc
tgttccccaa 6180 cgtgtcgcag caccattatc ccgatattct ggccatcatc
aagaacacct gcagcgagta 6240 caaggttcca taccttgtca aggatacgtt
ttggcaagca tttgcttcac atttggagca 6300 cttgcgtgtt cttggactcc
gtcccaagga agagtaggc 6339 <210> SEQ ID NO 76 <211>
LENGTH: 8319 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pY98 <400> SEQUENCE: 76 ggccgccacc gcggcccgag
attccggcct cttcggccgc caagcgaccc gggtggacgt 60 ctagaggtac
ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca 120
ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg
180 gtggagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
cgtaatcatg 240 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
attccacaca acgtacgagc 300 cggaagcata aagtgtaaag cctggggtgc
ctaatgagtg agctaactca cattaattgc 360 gttgcgctca ctgcccgctt
tccagtcggg aaacctgtcg tgccagctgc attaatgaat 420 cggccaacgc
gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 480
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
540 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
caaaaggcca 600 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
tttttccata ggctccgccc 660 ccctgacgag catcacaaaa atcgacgctc
aagtcagagg tggcgaaacc cgacaggact 720 ataaagatac caggcgtttc
cccctggaag ctccctcgtg cgctctcctg ttccgaccct 780 gccgcttacc
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 840
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
900 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
ttgagtccaa 960 cccggtaaga cacgacttat cgccactggc agcagccact
ggtaacagga ttagcagagc 1020 gaggtatgta ggcggtgcta cagagttctt
gaagtggtgg cctaactacg gctacactag 1080 aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa aaagagttgg 1140 tagctcttga
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1200
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
1260 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
tatcaaaaag 1320 gatcttcacc tagatccttt taaattaaaa atgaagtttt
aaatcaatct aaagtatata 1380 tgagtaaact tggtctgaca gttaccaatg
cttaatcagt gaggcaccta tctcagcgat 1440 ctgtctattt cgttcatcca
tagttgcctg actccccgtc gtgtagataa ctacgatacg 1500 ggagggctta
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 1560
tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
1620 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
taagtagttc 1680 gccagttaat agtttgcgca acgttgttgc cattgctaca
ggcatcgtgg tgtcacgctc 1740 gtcgtttggt atggcttcat tcagctccgg
ttcccaacga tcaaggcgag ttacatgatc 1800 ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 1860 gttggccgca
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 1920
gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
1980 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
ccgcgccaca 2040 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
tcggggcgaa aactctcaag 2100 gatcttaccg ctgttgagat ccagttcgat
gtaacccact cgtgcaccca actgatcttc 2160 agcatctttt actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 2220 aaaaaaggga
ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 2280
ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
2340 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
ctgacgcgcc 2400 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
cgcagcgtga ccgctacact 2460 tgccagcgcc ctagcgcccg ctcctttcgc
tttcttccct tcctttctcg ccacgttcgc 2520 cggctttccc cgtcaagctc
taaatcgggg gctcccttta gggttccgat ttagtgcttt 2580 acggcacctc
gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 2640
ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
2700 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
tataagggat 2760 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
taacaaaaat ttaacgcgaa 2820 ttttaacaaa atattaacgc ttacaatttc
cattcgccat tcaggctgcg caactgttgg 2880 gaagggcgat cggtgcgggc
ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 2940 gcaaggcgat
taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 3000
gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
3060 gtcgatggtg tcgataagct tgatatcgaa ttcatgtcac acaaaccgat
cttcgcctca 3120 aggaaaccta attctacatc cgagagactg ccgagatcca
gtctacactg attaattttc 3180 gggccaataa tttaaaaaaa tcgtgttata
taatattata tgtattatat atatacatca 3240 tgatgatact gacagtcatg
tcccattgct aaatagacag actccatctg ccgcctccaa 3300 ctgatgttct
caatatttaa ggggtcatct cgcattgttt aataataaac agactccatc 3360
taccgcctcc aaatgatgtt ctcaaaatat attgtatgaa cttattttta ttacttagta
3420 ttattagaca acttacttgc tttatgaaaa acacttccta tttaggaaac
aatttataat 3480 ggcagttcgt tcatttaaca atttatgtag aataaatgtt
ataaatgcgt atgggaaatc 3540 ttaaatatgg atagcataaa tgatatctgc
attgcctaat tcgaaatcaa cagcaacgaa 3600 aaaaatccct tgtacaacat
aaatagtcat cgagaaatat caactatcaa agaacagcta 3660 ttcacacgtt
actattgaga ttattattgg acgagaatca cacactcaac tgtctttctc 3720
tcttctagaa atacaggtac aagtatgtac tattctcatt gttcatactt ctagtcattt
3780 catcccacat attccttgga tttctctcca atgaatgaca ttctatcttg
caaattcaac 3840 aattataata agatatacca aagtagcggt atagtggcaa
tcaaaaagct tctctggtgt 3900 gcttctcgta tttattttta ttctaatgat
ccattaaagg tatatattta tttcttgtta 3960 tataatcctt ttgtttatta
catgggctgg atacataaag gtattttgat ttaatttttt 4020 gcttaaattc
aatcccccct cgttcagtgt caactgtaat ggtaggaaat taccatactt 4080
ttgaagaagc aaaaaaaatg aaagaaaaaa aaaatcgtat ttccaggtta gacgttccgc
4140 agaatctaga atgcggtatg cggtacattg ttcttcgaac gtaaaagttg
cgctccctga 4200 gatattgtac atttttgctt ttacaagtac aagtacatcg
tacaactatg tactactgtt 4260 gatgcatcca caacagtttg ttttgttttt
ttttgttttt tttttttcta atgattcatt 4320 accgctatgt atacctactt
gtacttgtag taagccgggt tattggcgtt caattaatca 4380 tagacttatg
aatctgcacg gtgtgcgctg cgagttactt ttagcttatg catgctactt 4440
gggtgtaata ttgggatctg ttcggaaatc aacggatgct caaccgattt cgacagtaat
4500 taattaagtc atacacaagt cagctttctt cgagcctcat ataagtataa
gtagttcaac 4560 gtattagcac tgtacccagc atctccgtat cgagaaacac
aacaacatgc cccattggac 4620 agatcatgcg gatacacagg ttgtgcagta
tcatacatac tcgatcagac aggtcgtctg 4680 accatcatac aagctgaaca
agcgctccat acttgcacgc tctctatata cacagttaaa 4740 ttacatatcc
atagtctaac ctctaacagt taatcttctg gtaagcctcc cagccagcct 4800
tctggtatcg cttggcctcc tcaataggat ctcggttctg gccgtacaga cctcggccga
4860 caattatgat atccgttccg gtagacatga catcctcaac agttcggtac
tgctgtccga 4920 gagcgtctcc cttgtcgtca agacccaccc cgggggtcag
aataagccag tcctcagagt 4980 cgcccttagg tcggttctgg gcaatgaagc
caaccacaaa ctcggggtcg gatcgggcaa 5040 gctcaatggt ctgcttggag
tactcgccag tggccagaga gcccttgcaa gacagctcgg 5100 ccagcatgag
cagacctctg gccagcttct cgttgggaga ggggactagg aactccttgt 5160
actgggagtt ctcgtagtca gagacgtcct ccttcttctg ttcagagaca gtttcctcgg
5220 caccagctcg caggccagca atgattccgg ttccgggtac accgtgggcg
ttggtgatat 5280 cggaccactc ggcgattcgg tgacaccggt actggtgctt
gacagtgttg ccaatatctg 5340 cgaactttct gtcctcgaac aggaagaaac
cgtgcttaag agcaagttcc ttgaggggga 5400 gcacagtgcc ggcgtaggtg
aagtcgtcaa tgatgtcgat atgggttttg atcatgcaca 5460 cataaggtcc
gaccttatcg gcaagctcaa tgagctcctt ggtggtggta acatccagag 5520
aagcacacag gttggttttc ttggctgcca cgagcttgag cactcgagcg gcaaaggcgg
5580 acttgtggac gttagctcga gcttcgtagg agggcatttt ggtggtgaag
aggagactga 5640 aataaattta gtctgcagaa ctttttatcg gaaccttatc
tggggcagtg aagtatatgt 5700 tatggtaata gttacgagtt agttgaactt
atagatagac tggactatac ggctatcggt 5760 ccaaattaga aagaacgtca
atggctctct gggcgtcgcc tttgccgaca aaaatgtgat 5820 catgatgaaa
gccagcaatg acgttgcagc tgatattgtt gtcggccaac cgcgccgaaa 5880
acgcagctgt cagacccaca gcctccaacg aagaatgtat cgtcaaagtg atccaagcac
5940 actcatagtt ggagtcgtac tccaaaggcg gcaatgacga gtcagacaga
tactcgtcga 6000 cgcagtagga tgtcctgcac gggtcttttt gtggggtgtg
gagaaagggg tgcttggaga 6060 tggaagccgg tagaaccggg ctgcttgtgc
ttggagatgg aagccggtag aaccgggctg 6120 cttgggggga tttggggccg
ctgggctcca aagaggggta ggcatttcgt tggggttacg 6180 taattgcggc
atttgggtcc tgcgcgcatg tcccattggt cagaattagt ccggatagga 6240
gacttatcag ccaatcacag cgccggatcc acctgtaggt tgggttgggt gggagcaccc
6300 ctccacagag tagagtcaaa cagcagcagc aacatgatag ttgggggtgt
gcgtgttaaa 6360 ggaaaaaaaa gaagcttggg ttatattccc gctctattta
gaggttgcgg gatagacgcc 6420 gacggagggc aatggcgcca tggaaccttg
cggatatcga tacgccgcgg cggactgcgt 6480 ccgaaccagc tccagcagcg
ttttttccgg gccattgagc cgactgcgac cccgccaacg 6540 tgtcttggcc
cacgcactca tgtcatgttg gtgttgggag gccacttttt aagtagcaca 6600
aggcacctag ctcgcagcaa ggtgtccgaa ccaaagaagc ggctgcagtg gtgcaaacgg
6660 ggcggaaacg gcgggaaaaa gccacggggg cacgaattga ggcacgccct
cgaatttgag 6720 acgagtcacg gccccattcg cccgcgcaat ggctcgccaa
cgcccggtct tttgcaccac 6780 atcaggttac cccaagccaa acctttgtgt
taaaaagctt aacatattat accgaacgta 6840 ggtttgggcg ggcttgctcc
gtctgtccaa ggcaacattt atataagggt ctgcatcgcc 6900 ggctcaattg
aatctttttt cttcttctct tctctatatt cattcttgaa ttaaacacac 6960
atcaatccgc ggccgcatgg gaacggacca aggaaaaacc ttcacctggg aagagctggc
7020 ggcccataac accaaggacg acctactctt ggccatccgc ggcagggtgt
acgatgtcac 7080 aaagttcttg agccgccatc ctggtggagt ggacactctc
ctgctcggag ctggccgaga 7140 tgttactccg gtctttgaga tgtatcacgc
gtttggggct gcagatgcca ttatgaagaa 7200 gtactatgtc ggtacactgg
tctcgaatga gctgcccatc ttcccggagc caacggtgtt 7260 ccacaaaacc
atcaagacga gagtcgaggg ctactttacg gatcggaaca ttgatcccaa 7320
gaatagacca gagatctggg gacgatacgc tcttatcttt ggatccttga tcgcttccta
7380 ctacgcgcag ctctttgtgc ctttcgttgt cgaacgcaca tggcttcagg
tggtgtttgc 7440 aatcatcatg ggatttgcgt gcgcacaagt cggactcaac
cctcttcatg atgcgtctca 7500 cttttcagtg acccacaacc ccactgtctg
gaagattctg ggagccacgc acgacttttt 7560 caacggagca tcgtacctgg
tgtggatgta ccaacatatg ctcggccatc acccctacac 7620 caacattgct
ggagcagatc ccgacgtgtc gacgtctgag cccgatgttc gtcgtatcaa 7680
gcccaaccaa aagtggtttg tcaaccacat caaccagcac atgtttgttc ctttcctgta
7740 cggactgctg gcgttcaagg tgcgcattca ggacatcaac attttgtact
ttgtcaagac 7800 caatgacgct attcgtgtca atcccatctc gacatggcac
actgtgatgt tctggggcgg 7860 caaggctttc tttgtctggt atcgcctgat
tgttcccctg cagtatctgc ccctgggcaa 7920 ggtgctgctc ttgttcacgg
tcgcggacat ggtgtcgtct tactggctgg cgctgacctt 7980 ccaggcgaac
cacgttgttg aggaagttca gtggccgttg cctgacgaga acgggatcat 8040
ccaaaaggac tgggcagcta tgcaggtcga gactacgcag gattacgcac acgattcgca
8100 cctctggacc agcatcactg gcagcttgaa ctaccaggct gtgcaccatc
tgttccccaa 8160 cgtgtcgcag caccattatc ccgatattct ggccatcatc
aagaacacct gcagcgagta 8220 caaggttcca taccttgtca aggatacgtt
ttggcaagca tttgcttcac atttggagca 8280 cttgcgtgtt cttggactcc
gtcccaagga agagtaggc 8319 <210> SEQ ID NO 77 <211>
LENGTH: 774 <212> TYPE: DNA <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 77 atggaggtgg tgaatgaaat agtctcaatt
gggcaggaag ttttacccaa agttgattat 60 gcccaactct ggagtgatgc
cagtcactgt gaggtgcttt acttgtccat cgcatttgtc 120 atcttgaagt
tcactcttgg cccccttggt ccaaaaggtc agtctcgtat gaagtttgtt 180
ttcaccaatt acaaccttct catgtccatt tattcgttgg gatcattcct ctcaatggca
240 tatgccatgt acaccatcgg tgttatgtct gacaactgcg agaaggcttt
tgacaacaac 300 gtcttcagga tcaccacgca gttgttctat ttgagcaagt
tcctggagta tattgactcc 360 ttctatttgc cactgatggg caagcctctg
acctggttgc aattcttcca tcatttgggg 420 gcaccgatgg atatgtggct
gttctataat taccgaaatg aagctgtttg gatttttgtg 480 ctgttgaatg
gtttcatcca ctggatcatg tacggttatt attggaccag attgatcaag 540
ctgaagttcc ccatgccaaa atccctgatt acatcaatgc agatcattca attcaatgtt
600 ggtttctaca ttgtctggaa gtacaggaac attccctgtt atcgccaaga
tgggatgagg 660 atgtttggct ggttcttcaa ttacttttat gttggcacag
tcttgtgttt gttcttgaat 720 ttctatgtgc aaacgtatat cgtcaggaag
cacaagggag ccaaaaagat tcag 774 <210> SEQ ID NO 78 <211>
LENGTH: 1263 <212> TYPE: DNA <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 78 atgaagtcaa agcgccaagc gcttcccctt
acaattgatg gaacaacata tgatgtgtct 60 gcctgggtca atttccaccc
tggtggtgcg gaaattatag agaattacca aggaagggat 120 gccactgatg
ccttcatggt tatgcactct caagaagcct tcgacaagct caagcgcatg 180
cccaaaatca atcccagttc tgagttgcca ccccaggctg cagtgaatga agctcaagag
240 gatttccgga agctccgaga agagttgatc gcaactggca tgtttgatgc
ctcccccctc 300 tggtactcat acaaaatcag caccacactg ggccttggag
tgctgggtta tttcctgatg 360 gttcagtatc agatgtattt cattggggca
gtgttgcttg ggatgcacta tcaacagatg 420 ggctggcttt ctcatgacat
ttgccaccac cagactttca agaaccggaa ctggaacaac 480 ctcgtgggac
tggtatttgg caatggtctg caaggttttt ccgtgacatg gtggaaggac 540
agacacaatg cacatcattc ggcaaccaat gttcaagggc acgaccctga tattgacaac
600 ctccccctct tagcctggtc tgaggatgac gtcacacggg cgtcaccgat
ttcccgcaag 660 ctcattcagt tccagcagta ctatttcttg gtcatctgta
tcttgttgcg gttcatttgg 720 tgtttccaga gcgtgttgac cgtgcgcagt
ttgaaggaca gagataacca attctatcgc 780 tctcagtata agaaggaggc
cattggcctc gccctgcact ggaccttgaa gaccctgttc 840 cacttattct
ttatgcccag catcctcaca tcgctgttgg tgtttttcgt ttcggagctg 900
gttggcggct tcggcattgc gatcgtggtg ttcatgaacc actacccact ggagaagatc
960 ggggactcag tctgggatgg ccatggattc tcggttggcc agatccatga
gaccatgaac 1020 attcggcgag ggattatcac agattggttt ttcggaggct
tgaattacca gattgagcac 1080 catttgtggc cgaccctccc tcgccacaac
ctgacagcgg ttagctacca ggtggaacag 1140 ctgtgccaga agcacaacct
gccgtatcgg aacccgctgc cccatgaagg gttggtcatc 1200 ctgctgcgct
atctggcggt gttcgcccgg atggcggaga agcaacccgc ggggaaggct 1260 cta
1263 <210> SEQ ID NO 79 <211> LENGTH: 30 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Primer oEugEL1-1
<400> SEQUENCE: 79 agcggccgca ccatggaggt ggtgaatgaa 30
<210> SEQ ID NO 80 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer oEugEL1-2 <400>
SEQUENCE: 80 tgcggccgct cactgaatct ttttggctcc 30 <210> SEQ ID
NO 81 <211> LENGTH: 4311 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR906 <400> SEQUENCE: 81
agcggccgca ccatggaggt ggtgaatgaa atagtctcaa ttgggcagga agttttaccc
60 aaagttgatt atgcccaact ctggagtgat gccagtcact gtgaggtgct
ttacttgtcc 120 atcgcatttg tcatcttgaa gttcactctt ggcccccttg
gtccaaaagg tcagtctcgt 180 atgaagtttg ttttcaccaa ttacaacctt
ctcatgtcca tttattcgtt gggatcattc 240 ctctcaatgg catatgccat
gtacaccatc ggtgttatgt ctgacaactg cgagaaggct 300 tttgacaaca
acgtcttcag gatcaccacg cagttgttct atttgagcaa gttcctggag 360
tatattgact ccttctattt gccactgatg ggcaagcctc tgacctggtt gcaattcttc
420 catcatttgg gggcaccgat ggatatgtgg ctgttctata attaccgaaa
tgaagctgtt 480 tggatttttg tgctgttgaa tggtttcatc cactggatca
tgtacggtta ttattggacc 540 agattgatca agctgaagtt ccccatgcca
aaatccctga ttacatcaat gcagatcatt 600 caattcaatg ttggtttcta
cattgtctgg aagtacagga acattccctg ttatcgccaa 660 gatgggatga
ggatgtttgg ctggttcttc aattactttt atgttggcac agtcttgtgt 720
ttgttcttga atttctatgt gcaaacgtat atcgtcagga agcacaaggg agccaaaaag
780 attcagtgag cggccgcacc tgaattccag cacactggcg gccgttacta
gtggatccga 840 gctcggtacc aagcttgatg catagcttga gtattctaac
gcgtcaccta aatagcttgg 900 cgtaatcatg gtcatagctg tttcctgtgt
gaaattgtta tccgctcaca attccacaca 960 acatacgagc cggaagcata
aagtgtaaag cctggggtgc ctaatgagtg agctaactca 1020 cattaattgc
gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 1080
attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
1140 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
tcagctcact 1200 caaaggcggt aatacggtta tccacagaat caggggataa
cgcaggaaag aacatgtgag 1260 caaaaggcca gcaaaagccc aggaaccgta
aaaaggccgc gttgctggcg tttttccata 1320 ggctccgccc ccctgacgag
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 1380 cgacaggact
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 1440
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
1500 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
tccaagctgg 1560 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
cttatccggt aactatcgtc 1620 ttgagtccaa cccggtaaga cacgacttat
cgccactggc agcagccact ggtaacagga 1680 ttagcagagc gaggtatgta
ggcggtgcta cagagttctt gaagtggtgg cctaactacg 1740 gctacactag
aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 1800
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
1860 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
ttgatctttt 1920 ctacggggtc tgacgctcag tggaacgaaa actcacgtta
agggattttg gtcatgagat 1980 tatcaaaaag gatcttcacc tagatccttt
taaattaaaa atgaagtttt agcacgtgtc 2040 agtcctgctc ctcggccacg
aagtgcacgc agttgccggc cgggtcgcgc agggcgaact 2100 cccgccccca
cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt 2160
tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
2220 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac
agggtcacgt 2280 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc
ccgggagaac ccgagccggt 2340 cggtccagaa ctcgaccgct ccggcgacgt
cgcgcgcggt gagcaccgga acggcactgg 2400 tcaacttggc catggtggcc
ctcctcacgt gctattattg aagcatttat cagggttatt 2460 gtctcatgag
cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 2520
gcacatttcc ccgaaaagtg ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag
2580 gagaaaatac cgcatcagga aattgtaagc gttaataatt cagaagaact
cgtcaagaag 2640 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
ccgtaaagca cgaggaagcg 2700 gtcagcccat tcgccgccaa gctcttcagc
aatatcacgg gtagccaacg ctatgtcctg 2760 atagcggtcc gccacaccca
gccggccaca gtcgatgaat ccagaaaagc ggccattttc 2820 caccatgata
ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg 2880
catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
2940 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
cgatgcgatg 3000 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
gtatgcagcc gccgcattgc 3060 atcagccatg atggatactt tctcggcagg
agcaaggtga gatgacagga gatcctgccc 3120 cggcacttcg cccaatagca
gccagtccct tcccgcttca gtgacaacgt cgagcacagc 3180 tgcgcaagga
acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc 3240
attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
3300 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
agccgaatag 3360 cctctccacc caagcggccg gagaacctgc gtgcaatcca
tcttgttcaa tcatgcgaaa 3420 cgatcctcat cctgtctctt gatcagagct
tgatcccctg cgccatcaga tccttggcgg 3480 cgagaaagcc atccagttta
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc 3540 tggcaattcc
ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag 3600
cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
3660 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
acgtgaaaag 3720 gatctaggtg aagatccttt ttgataatct catgcctgac
atttatattc cccagaacat 3780 caggttaatg gcgtttttga tgtcattttc
gcggtggctg agatcagcca cttcttcccc 3840 gataacggag accggcacac
tggccatatc ggtggtcatc atgcgccagc tttcatcccc 3900 gatatgcacc
accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc 3960
cagggggatc accatccgtc gccccggcgt gtcaataata tcactctgta catccacaaa
4020 cagacgataa cggctctctc ttttataggt gtaaacctta aactgccgta
cgtataggct 4080 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
ctattacgcc agctggcgaa 4140 agggggatgt gctgcaaggc gattaagttg
ggtaacgcca gggttttccc agtcacgacg 4200 ttgtaaaacg acggccagtg
aattgtaata cgactcacta tagggcgaat tgggccctct 4260 agatgcatgc
tcgagcggcc gccagtgtga tggatatctg cagaattcag g 4311 <210> SEQ
ID NO 82 <211> LENGTH: 7085 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR72 <400> SEQUENCE: 82
gtacggatcc gtcgacggcg cgcccgatca tccggatata gttcctcctt tcagcaaaaa
60 acccctcaag acccgtttag aggccccaag gggttatgct agttattgct
cagcggtggc 120 agcagccaac tcagcttcct ttcgggcttt gttagcagcc
ggatcgatcc aagctgtacc 180 tcactattcc tttgccctcg gacgagtgct
ggggcgtcgg tttccactat cggcgagtac 240 ttctacacag ccatcggtcc
agacggccgc gcttctgcgg gcgatttgtg tacgcccgac 300 agtcccggct
ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc 360
gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
420 ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
gcgtctgctg 480 ctccatacaa gccaaccacg gcctccagaa gaagatgttg
gcgacctcgt attgggaatc 540 cccgaacatc gcctcgctcc agtcaatgac
cgctgttatg cggccattgt ccgtcaggac 600 attgttggag ccgaaatccg
cgtgcacgag gtgccggact tcggggcagt cctcggccca 660 aagcatcagc
tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt 720
ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
780 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
gatcggccgc 840 agcgatcgca tccatagcct ccgcgaccgg ctgcagaaca
gcgggcagtt cggtttcagg 900 caggtcttgc aacgtgacac cctgtgcacg
gcgggagatg caataggtca ggctctcgct 960 gaattcccca atgtcaagca
cttccggaat cgggagcgcg gccgatgcaa agtgccgata 1020 aacataacga
tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg 1080
ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
1140 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
tgagttcagg 1200 cttttccatg ggtatatctc cttcttaaag ttaaacaaaa
ttatttctag agggaaaccg 1260 ttgtggtctc cctatagtga gtcgtattaa
tttcgcggga tcgagatcga tccaattcca 1320 atcccacaaa aatctgagct
taacagcaca gttgctcctc tcagagcaga atcgggtatt 1380 caacaccctc
atatcaacta ctacgttgtg tataacggtc cacatgccgg tatatacgat 1440
gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt
1500 gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca
gcaaacagac 1560 aggttgaact tcatccccaa aggagaagct caactcaagc
ccaagagctt tgctaaggcc 1620 ctaacaagcc caccaaagca aaaagcccac
tggctcacgc taggaaccaa aaggcccagc 1680 agtgatccag ccccaaaaga
gatctccttt gccccggaga ttacaatgga cgatttcctc 1740 tatctttacg
atctaggaag gaagttcgaa ggtgaaggtg acgacactat gttcaccact 1800
gataatgaga aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
1860 gaggcctacg cagcaggtct catcaagacg atctacccga gtaacaatct
ccaggagatc 1920 aaataccttc ccaagaaggt taaagatgca gtcaaaagat
tcaggactaa ttgcatcaag 1980 aacacagaga aagacatatt tctcaagatc
agaagtacta ttccagtatg gacgattcaa 2040 ggcttgcttc ataaaccaag
gcaagtaata gagattggag tctctaaaaa ggtagttcct 2100 actgaatcta
aggccatgca tggagtctaa gattcaaatc gaggatctaa cagaactcgc 2160
cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
2220 cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca
aagatacagt 2280 ctcagaagac caaagggcta ttgagacttt tcaacaaagg
ataatttcgg gaaacctcct 2340 cggattccat tgcccagcta tctgtcactt
catcgaaagg acagtagaaa aggaaggtgg 2400 ctcctacaaa tgccatcatt
gcgataaagg aaaggctatc attcaagatg cctctgccga 2460 cagtggtccc
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc 2520
aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
2580 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
ttcatttgga 2640 gaggacacgc tcgagctcat ttctctatta cttcagccat
aacaaaagaa ctcttttctc 2700 ttcttattaa accatgaaaa agcctgaact
caccgcgacg tctgtcgaga agtttctgat 2760 cgaaaagttc gacagcgtct
ccgacctgat gcagctctcg gagggcgaag aatctcgtgc 2820 tttcagcttc
gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg 2880
tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
2940 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
gccgtgcaca 3000 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
gctgttctgc agccggtcgc 3060 ggaggccatg gatgcgatcg ctgcggccga
tcttagccag acgagcgggt tcggcccatt 3120 cggaccgcaa ggaatcggtc
aatacactac atggcgtgat ttcatatgcg cgattgctga 3180 tccccatgtg
tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 3240
ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
3300 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
cggtcattga 3360 ctggagcgag gcgatgttcg gggattccca atacgaggtc
gccaacatct tcttctggag 3420 gccgtggttg gcttgtatgg agcagcagac
gcgctacttc gagcggaggc atccggagct 3480 tgcaggatcg ccgcggctcc
gggcgtatat gctccgcatt ggtcttgacc aactctatca 3540 gagcttggtt
gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat 3600
cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
3660 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
ccagcactcg 3720 tccgagggca aaggaatagt gaggtaccta aagaaggagt
gcgtcgaagc agatcgttca 3780 aacatttggc aataaagttt cttaagattg
aatcctgttg ccggtcttgc gatgattatc 3840 atataatttc tgttgaatta
cgttaagcat gtaataatta acatgtaatg catgacgtta 3900 tttatgagat
gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa 3960
aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
4020 gatcgatgtc gaatcgatca acctgcatta atgaatcggc caacgcgcgg
ggagaggcgg 4080 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
tcgctgcgct cggtcgttcg 4140 gctgcggcga gcggtatcag ctcactcaaa
ggcggtaata cggttatcca cagaatcagg 4200 ggataacgca ggaaagaaca
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 4260 ggccgcgttg
ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 4320
acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4380 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
acctgtccgc 4440 ctttctccct tcgggaagcg tggcgctttc tcaatgctca
cgctgtaggt atctcagttc 4500 ggtgtaggtc gttcgctcca agctgggctg
tgtgcacgaa ccccccgttc agcccgaccg 4560 ctgcgcctta tccggtaact
atcgtcttga gtccaacccg gtaagacacg acttatcgcc 4620 actggcagca
gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 4680
gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
4740 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
gcaaacaaac 4800 caccgctggt agcggtggtt tttttgtttg caagcagcag
attacgcgca gaaaaaaagg 4860 atctcaagaa gatcctttga tcttttctac
ggggtctgac gctcagtgga acgaaaactc 4920 acgttaaggg attttggtca
tgacattaac ctataaaaat aggcgtatca cgaggccctt 4980 tcgtctcgcg
cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5040
ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
5100 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
ttgtactgag 5160 agtgcaccat atggacatat tgtcgttaga acgcggctac
aattaataca taaccttatg 5220 tatcatacac atacgattta ggtgacacta
tagaacggcg cgccaagctt gttgaaacat 5280 ccctgaagtg tctcatttta
ttttatttat tctttgctga taaaaaaata aaataaaaga 5340 agctaagcac
acggtcaacc attgctctac tgctaaaagg gttatgtgta gtgttttact 5400
gcataaatta tgcagcaaac aagacaactc aaattaaaaa atttcctttg cttgtttttt
5460 tgttgtctct gacttgactt tcttgtggaa gttggttgta taaggattgg
gacaccattg 5520 tccttcttaa tttaatttta ttctttgctg ataaaaaaaa
aaatttcata tagtgttaaa 5580 taataatttg ttaaataacc aaaaagtcaa
atatgtttac tctcgtttaa ataattgaga 5640 ttcgtccagc aaggctaaac
gattgtatag atttatgaca atatttactt ttttatagat 5700 aaatgttata
ttataataaa tttatataca tatattatat gttatttatt attattttaa 5760
atccttcaat attttatcaa accaactcat aatttttttt ttatctgtaa gaagcaataa
5820 aattaaatag acccacttta aggatgatcc aacctttata cagagtaaga
gagttcaaat 5880 agtacccttt catatacata tcaactaaaa tattagaaat
atcatggatc aaaccttata 5940 aagacattaa ataagtggat aagtataata
tataaatggg tagtatataa tatataaatg 6000 gatacaaact tctctcttta
taattgttat gtctccttaa catcctaata taatacataa 6060 gtgggtaata
tataatatat aaatggagac aaacttcttc cattataatt gttatgtctt 6120
cttaacactt atgtctcgtt cacaatgcta aggttagaat tgtttagaaa gtcttatagt
6180 acacatttgt ttttgtacta tttgaagcat tccataagcc gtcacgattc
agatgattta 6240 taataataag aggaaattta tcatagaaca ataaggtgca
tagatagagt gttaatatat 6300 cataacatcc tttgtttatt catagaagaa
gtgagatgga gctcagttat tatactgtta 6360 catggtcgga tacaatattc
catgctctcc atgagctctt acacctacat gcattttagt 6420 tcatacttgc
ggccgcagta tatcttaaat tctttaatac ggtgtactag gatattgaac 6480
tggttcttga tgatgaaaac ctgggccgag attgcagcta tttatagtca taggtcttgt
6540 taacatgcat ggacatttgg ccacggggtg gcatgcagtt tgacgggtgt
tgaaataaac 6600 aaaaatgagg tggcggaaga gaatacgagt ttgaggttgg
gttagaaaca acaaatgtga 6660 gggctcatga tgggttgagt tggtgaatgt
tttgggctgc tcgattgaca cctttgtgag 6720 tacgtgttgt tgtgcatggc
ttttggggtc cagttttttt ttcttgacgc ggcgatcctg 6780 atcagctagt
ggataagtga tgtccactgt gtgtgattgc gtttttgttt gaattttatg 6840
aacttagaca ttgctatgca aaggatactc tcattgtgtt ttgtcttctt ttgttccttg
6900 gctttttctt atgatccaag agactagtca gtgttgtggc attcgagact
accaagatta 6960 attatgatgg gggaaggata agtaactgat tagtacggac
tgttaccaaa ttaattaata 7020 agcggcaaat gaagggcatg gatcaaaagc
ttggatctcc tgcaggatct ggccggccgg 7080 atctc 7085 <210> SEQ ID
NO 83 <211> LENGTH: 2540 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKS102 <400> SEQUENCE: 83
cgatcatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc
60 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
cttcctttcg 120 ggctttgtta gcagccggat cgatccaagc tgtacctcac
tattcctttg ccctcggacg 180 agtgctgggg cgtcggtttc cactatcggc
gagtacttct acacagccat cggtccagac 240 ggccgcgctt ctgcgggcga
tttgtgtacg cccgacagtc ccggctccgg atcggacgat 300 tgcgtcgcat
cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg 360
atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
420 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
accacggcct 480 ccagaagaag atgttggcga cctcgtattg ggaatccccg
aacatcgcct cgctccagtc 540 aatgaccgct gttatgcggc cattgtccgt
caggacattg ttggagccga aatccgcgtg 600 cacgaggtgc cggacttcgg
ggcagtcctc ggcccaaagc atcagctcat cgagagcctg 660 cgcgacggac
gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc 720
agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
780 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
tagcctccgc 840 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg
tcttgcaacg tgacaccctg 900 tgcacggcgg gagatgcaat aggtcaggct
ctcgctgaat tccccaatgt caagcacttc 960 cggaatcggg agcgcggccg
atgcaaagtg ccgataaaca taacgatctt tgtagaaacc 1020 atcggcgcag
ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc 1080
acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
1140 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt tccatgggta
tatctccttc 1200 ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
ggtctcccta tagtgagtcg 1260 tattaatttc gcgggatcga gatctgatca
acctgcatta atgaatcggc caacgcgcgg 1320 ggagaggcgg tttgcgtatt
gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 1380 cggtcgttcg
gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 1440
cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
1500 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
gacgagcatc 1560 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
aggactataa agataccagg 1620 cgtttccccc tggaagctcc ctcgtgcgct
ctcctgttcc gaccctgccg cttaccggat 1680 acctgtccgc ctttctccct
tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 1740 atctcagttc
ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 1800
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
1860 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
tatgtaggcg 1920 gtgctacaga gttcttgaag tggtggccta actacggcta
cactagaagg acagtatttg 1980 gtatctgcgc tctgctgaag ccagttacct
tcggaaaaag agttggtagc tcttgatccg 2040 gcaaacaaac caccgctggt
agcggtggtt tttttgtttg caagcagcag attacgcgca 2100 gaaaaaaagg
atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 2160
acgaaaactc acgttaaggg attttggtca tgacattaac ctataaaaat aggcgtatca
2220 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
cacatgcagc 2280 tcccggagac ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg 2340 gcgcgtcagc gggtgttggc gggtgtcggg
gctggcttaa ctatgcggca tcagagcaga 2400 ttgtactgag agtgcaccat
atggacatat tgtcgttaga acgcggctac aattaataca 2460 taaccttatg
tatcatacac atacgattta ggtgacacta tagaacggcg cgccaagctt 2520
ggatccgtcg acggcgcgcc 2540 <210> SEQ ID NO 84 <211>
LENGTH: 4359 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pKR197 <400> SEQUENCE: 84 cgcgcccgat catccggata
tagttcctcc tttcagcaaa aaacccctca agacccgttt 60 agaggcccca
aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc 120
ctttcgggct ttgttagcag ccggatcgat ccaagctgta cctcactatt cctttgccct
180 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
agccatcggt 240 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
acagtcccgg ctccggatcg 300 gacgattgcg tcgcatcgac cctgcgccca
agctgcatca tcgaaattgc cgtcaaccaa 360 gctctgatag agttggtcaa
gaccaatgcg gagcatatac gcccggagcc gcggcgatcc 420 tgcaagctcc
ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca 480
cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
540 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
agccgaaatc 600 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
caaagcatca gctcatcgag 660 agcctgcgcg acggacgcac tgacggtgtc
gtccatcaca gtttgccagt gatacacatg 720 gggatcagca atcgcgcata
tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg 780 tccgaatggg
ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc 840
ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
900 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc
caatgtcaag 960 cacttccgga atcgggagcg cggccgatgc aaagtgccga
taaacataac gatctttgta 1020 gaaaccatcg gcgcagctat ttacccgcag
gacatatcca cgccctccta catcgaagct 1080 gaaagcacga gattcttcgc
cctccgagag ctgcatcagg tcggagacgc tgtcgaactt 1140 ttcgatcaga
aacttctcga cagacgtcgc ggtgagttca ggcttttcca tgggtatatc 1200
tccttcttaa agttaaacaa aattatttct agagggaaac cgttgtggtc tccctatagt
1260 gagtcgtatt aatttcgcgg gatcgagatc tgatcaacct gcattaatga
atcggccaac 1320 gcgcggggag aggcggtttg cgtattgggc gctcttccgc
ttcctcgctc actgactcgc 1380 tgcgctcggt cgttcggctg cggcgagcgg
tatcagctca ctcaaaggcg gtaatacggt 1440 tatccacaga atcaggggat
aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 1500 ccaggaaccg
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 1560
agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
1620 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
ctgccgctta 1680 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
gctttctcaa tgctcacgct 1740 gtaggtatct cagttcggtg taggtcgttc
gctccaagct gggctgtgtg cacgaacccc 1800 ccgttcagcc cgaccgctgc
gccttatccg gtaactatcg tcttgagtcc aacccggtaa 1860 gacacgactt
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 1920
taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
1980 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
ggtagctctt 2040 gatccggcaa acaaaccacc gctggtagcg gtggtttttt
tgtttgcaag cagcagatta 2100 cgcgcagaaa aaaaggatct caagaagatc
ctttgatctt ttctacgggg tctgacgctc 2160 agtggaacga aaactcacgt
taagggattt tggtcatgac attaacctat aaaaataggc 2220 gtatcacgag
gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 2280
tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
2340 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
gcggcatcag 2400 agcagattgt actgagagtg caccatatgg acatattgtc
gttagaacgc ggctacaatt 2460 aatacataac cttatgtatc atacacatac
gatttaggtg acactataga acggcgcgcc 2520 aagcttgttg aaacatccct
gaagtgtctc attttatttt atttattctt tgctgataaa 2580 aaaataaaat
aaaagaagct aagcacacgg tcaaccattg ctctactgct aaaagggtta 2640
tgtgtagtgt tttactgcat aaattatgca gcaaacaaga caactcaaat taaaaaattt
2700 cctttgcttg tttttttgtt gtctctgact tgactttctt gtggaagttg
gttgtataag 2760 gattgggaca ccattgtcct tcttaattta attttattct
ttgctgataa aaaaaaaaat 2820 ttcatatagt gttaaataat aatttgttaa
ataaccaaaa agtcaaatat gtttactctc 2880 gtttaaataa ttgagattcg
tccagcaagg ctaaacgatt gtatagattt atgacaatat 2940 ttactttttt
atagataaat gttatattat aataaattta tatacatata ttatatgtta 3000
tttattatta ttttaaatcc ttcaatattt tatcaaacca actcataatt ttttttttat
3060 ctgtaagaag caataaaatt aaatagaccc actttaagga tgatccaacc
tttatacaga 3120 gtaagagagt tcaaatagta ccctttcata tacatatcaa
ctaaaatatt agaaatatca 3180 tggatcaaac cttataaaga cattaaataa
gtggataagt ataatatata aatgggtagt 3240 atataatata taaatggata
caaacttctc tctttataat tgttatgtct ccttaacatc 3300 ctaatataat
acataagtgg gtaatatata atatataaat ggagacaaac ttcttccatt 3360
ataattgtta tgtcttctta acacttatgt ctcgttcaca atgctaaggt tagaattgtt
3420 tagaaagtct tatagtacac atttgttttt gtactatttg aagcattcca
taagccgtca 3480 cgattcagat gatttataat aataagagga aatttatcat
agaacaataa ggtgcataga 3540 tagagtgtta atatatcata acatcctttg
tttattcata gaagaagtga gatggagctc 3600 agttattata ctgttacatg
gtcggataca atattccatg ctctccatga gctcttacac 3660 ctacatgcat
tttagttcat acttgcggcc gcagtatatc ttaaattctt taatacggtg 3720
tactaggata ttgaactggt tcttgatgat gaaaacctgg gccgagattg cagctattta
3780 tagtcatagg tcttgttaac atgcatggac atttggccac ggggtggcat
gcagtttgac 3840 gggtgttgaa ataaacaaaa atgaggtggc ggaagagaat
acgagtttga ggttgggtta 3900 gaaacaacaa atgtgagggc tcatgatggg
ttgagttggt gaatgttttg ggctgctcga 3960 ttgacacctt tgtgagtacg
tgttgttgtg catggctttt ggggtccagt ttttttttct 4020 tgacgcggcg
atcctgatca gctagtggat aagtgatgtc cactgtgtgt gattgcgttt 4080
ttgtttgaat tttatgaact tagacattgc tatgcaaagg atactctcat tgtgttttgt
4140 cttcttttgt tccttggctt tttcttatga tccaagagac tagtcagtgt
tgtggcattc 4200 gagactacca agattaatta tgatggggga aggataagta
actgattagt acggactgtt 4260 accaaattaa ttaataagcg gcaaatgaag
ggcatggatc aaaagcttgg atctcctgca 4320 ggatctggcc ggccggatct
cgtacggatc cgtcgacgg 4359 <210> SEQ ID NO 85 <211>
LENGTH: 5147 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pKR911 <400> SEQUENCE: 85 ggccgcaagt atgaactaaa
atgcatgtag gtgtaagagc tcatggagag catggaatat 60 tgtatccgac
catgtaacag tataataact gagctccatc tcacttcttc tatgaataaa 120
caaaggatgt tatgatatat taacactcta tctatgcacc ttattgttct atgataaatt
180 tcctcttatt attataaatc atctgaatcg tgacggctta tggaatgctt
caaatagtac 240 aaaaacaaat gtgtactata agactttcta aacaattcta
accttagcat tgtgaacgag 300 acataagtgt taagaagaca taacaattat
aatggaagaa gtttgtctcc atttatatat 360 tatatattac ccacttatgt
attatattag gatgttaagg agacataaca attataaaga 420 gagaagtttg
tatccattta tatattatat actacccatt tatatattat acttatccac 480
ttatttaatg tctttataag gtttgatcca tgatatttct aatattttag ttgatatgta
540 tatgaaaggg tactatttga actctcttac tctgtataaa ggttggatca
tccttaaagt 600 gggtctattt aattttattg cttcttacag ataaaaaaaa
aattatgagt tggtttgata 660 aaatattgaa ggatttaaaa taataataaa
taacatataa tatatgtata taaatttatt 720 ataatataac atttatctat
aaaaaagtaa atattgtcat aaatctatac aatcgtttag 780 ccttgctgga
cgaatctcaa ttatttaaac gagagtaaac atatttgact ttttggttat 840
ttaacaaatt attatttaac actatatgaa attttttttt ttatcagcaa agaataaaat
900 taaattaaga aggacaatgg tgtcccaatc cttatacaac caacttccac
aagaaagtca 960 agtcagagac aacaaaaaaa caagcaaagg aaatttttta
atttgagttg tcttgtttgc 1020 tgcataattt atgcagtaaa acactacaca
taaccctttt agcagtagag caatggttga 1080 ccgtgtgctt agcttctttt
attttatttt tttatcagca aagaataaat aaaataaaat 1140 gagacacttc
agggatgttt caacaagctt ggcgcgccgt tctatagtgt cacctaaatc 1200
gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
1260 ccatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
cagccccgac 1320 acccgccaac acccgctgac gcgccctgac gggcttgtct
gctcccggca tccgcttaca 1380 gacaagctgt gaccgtctcc gggagctgca
tgtgtcagag gttttcaccg tcatcaccga 1440 aacgcgcgag acgaaagggc
ctcgtgatac gcctattttt ataggttaat gtcatgacca 1500 aaatccctta
acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 1560
gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1620 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
ccgaaggtaa 1680 ctggcttcag cagagcgcag ataccaaata ctgtccttct
agtgtagccg tagttaggcc 1740 accacttcaa gaactctgta gcaccgccta
catacctcgc tctgctaatc ctgttaccag 1800 tggctgctgc cagtggcgat
aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 1860 cggataaggc
gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 1920
gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
1980 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
ggagagcgca 2040 cgagggagct tccaggggga aacgcctggt atctttatag
tcctgtcggg tttcgccacc 2100 tctgacttga gcgtcgattt ttgtgatgct
cgtcaggggg gcggagccta tggaaaaacg 2160 ccagcaacgc ggccttttta
cggttcctgg ccttttgctg gccttttgct cacatgttct 2220 ttcctgcgtt
atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 2280
ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
2340 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
aggttgatca 2400 gatctcgatc ccgcgaaatt aatacgactc actataggga
gaccacaacg gtttccctct 2460 agaaataatt ttgtttaact ttaagaagga
gatataccca tggaaaagcc tgaactcacc 2520 gcgacgtctg tcgagaagtt
tctgatcgaa aagttcgaca gcgtctccga cctgatgcag 2580 ctctcggagg
gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc 2640
ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
2700 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
cgagagcctg 2760 acctattgca tctcccgccg tgcacagggt gtcacgttgc
aagacctgcc tgaaaccgaa 2820 ctgcccgctg ttctgcagcc ggtcgcggag
gctatggatg cgatcgctgc ggccgatctt 2880 agccagacga gcgggttcgg
cccattcgga ccgcaaggaa tcggtcaata cactacatgg 2940 cgtgatttca
tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac 3000
gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
3060 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
cctgacggac 3120 aatggccgca taacagcggt cattgactgg agcgaggcga
tgttcgggga ttcccaatac 3180 gaggtcgcca acatcttctt ctggaggccg
tggttggctt gtatggagca gcagacgcgc 3240 tacttcgagc ggaggcatcc
ggagcttgca ggatcgccgc ggctccgggc gtatatgctc 3300 cgcattggtc
ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct 3360
tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
3420 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
actcgccgat 3480 agtggaaacc gacgccccag cactcgtccg agggcaaagg
aatagtgagg tacagcttgg 3540 atcgatccgg ctgctaacaa agcccgaaag
gaagctgagt tggctgctgc caccgctgag 3600 caataactag cataacccct
tggggcctct aaacgggtct tgaggggttt tttgctgaaa 3660 ggaggaacta
tatccggatg atcgggcgcg ccgtcgacgg atccgtacga gatccggccg 3720
gccagatcct gcaggagatc caagcttttg atccatgccc ttcatttgcc gcttattaat
3780 taatttggta acagtccgta ctaatcagtt acttatcctt cccccatcat
aattaatctt 3840 ggtagtctcg aatgccacaa cactgactag tctcttggat
cataagaaaa agccaaggaa 3900 caaaagaaga caaaacacaa tgagagtatc
ctttgcatag caatgtctaa gttcataaaa 3960 ttcaaacaaa aacgcaatca
cacacagtgg acatcactta tccactagct gatcaggatc 4020 gccgcgtcaa
gaaaaaaaaa ctggacccca aaagccatgc acaacaacac gtactcacaa 4080
aggtgtcaat cgagcagccc aaaacattca ccaactcaac ccatcatgag ccctcacatt
4140 tgttgtttct aacccaacct caaactcgta ttctcttccg ccacctcatt
tttgtttatt 4200 tcaacacccg tcaaactgca tgccaccccg tggccaaatg
tccatgcatg ttaacaagac 4260 ctatgactat aaatagctgc aatctcggcc
caggttttca tcatcaagaa ccagttcaat 4320 atcctagtac accgtattaa
agaatttaag atatactgcg gccgcaccat ggaggtggtg 4380 aatgaaatag
tctcaattgg gcaggaagtt ttacccaaag ttgattatgc ccaactctgg 4440
agtgatgcca gtcactgtga ggtgctttac ttgtccatcg catttgtcat cttgaagttc
4500 actcttggcc cccttggtcc aaaaggtcag tctcgtatga agtttgtttt
caccaattac 4560 aaccttctca tgtccattta ttcgttggga tcattcctct
caatggcata tgccatgtac 4620 accatcggtg ttatgtctga caactgcgag
aaggcttttg acaacaacgt cttcaggatc 4680 accacgcagt tgttctattt
gagcaagttc ctggagtata ttgactcctt ctatttgcca 4740 ctgatgggca
agcctctgac ctggttgcaa ttcttccatc atttgggggc accgatggat 4800
atgtggctgt tctataatta ccgaaatgaa gctgtttgga tttttgtgct gttgaatggt
4860 ttcatccact ggatcatgta cggttattat tggaccagat tgatcaagct
gaagttcccc 4920 atgccaaaat ccctgattac atcaatgcag atcattcaat
tcaatgttgg tttctacatt 4980 gtctggaagt acaggaacat tccctgttat
cgccaagatg ggatgaggat gtttggctgg 5040 ttcttcaatt acttttatgt
tggcacagtc ttgtgtttgt tcttgaattt ctatgtgcaa 5100 acgtatatcg
tcaggaagca caagggagcc aaaaagattc agtgagc 5147 <210> SEQ ID NO
86 <211> LENGTH: 6559 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR680 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (4340)..(4340)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 86 ggccgcgaca caagtgtgag agtactaaat aaatgctttg gttgtacgaa
atcattacac 60 taaataaaat aatcaaagct tatatatgcc ttccgctaag
gccgaatgca aagaaattgg 120 ttctttctcg ttatcttttg ccacttttac
tagtacgtat taattactac ttaatcatct 180 ttgtttacgg ctcattatat
ccggtctaga ggatccaagg ccgcgaagtt aaaagcaatg 240 ttgtcacttg
tcgtactaac acatgatgtg atagtttatg ctagctagct ataacataag 300
ctgtctctga gtgtgttgta tattaataaa gatcatcact ggtgaatggt gatcgtgtac
360 gtaccctact tagtaggcaa tggaagcact tagagtgtgc tttgtgcatg
gccttgcctc 420 tgttttgaga cttttgtaat gttttcgagt ttaaatcttt
gcctttgcgt acgtgggcgg 480 atcccccggg ctgcaggaat tcactggccg
tcgttttaca acgtcgtgac tgggaaaacc 540 ctggcgttac ccaacttaat
cgccttgcag cacatccccc tttcgccagc tggcgtaata 600 gcgaagaggc
ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 660
gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
720 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
ccgccaacac 780 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
cgcttacaga caagctgtga 840 ccgtctccgg gagctgcatg tgtcagaggt
tttcaccgtc atcaccgaaa cgcgcgagac 900 gaaagggcct cgtgatacgc
ctatttttat aggttaatgt catgataata atggtttctt 960 agacgtcagg
tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 1020
aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
1080 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
cccttttttg 1140 cggcattttg ccttcctgtt tttgctcacc cagaaacgct
ggtgaaagta aaagatgctg 1200 aagatcagtt gggtgcacga gtgggttaca
tcgaactgga tctcaacagc ggtaagatcc 1260 ttgagagttt tcgccccgaa
gaacgttttc caatgatgag cacttttaaa gttctgctat 1320 gtggcgcggt
attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 1380
attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
1440 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
gcggccaact 1500 tacttctgac aacgatcgga ggaccgaagg agctaaccgc
ttttttgcac aacatggggg 1560 atcatgtaac tcgccttgat cgttgggaac
cggagctgaa tgaagccata ccaaacgacg 1620 agcgtgacac cacgatgcct
gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 1680 aactacttac
tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 1740
caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
1800 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
aagccctccc 1860 gtatcgtagt tatctacacg acggggagtc aggcaactat
ggatgaacga aatagacaga 1920 tcgctgagat aggtgcctca ctgattaagc
attggtaact gtcagaccaa gtttactcat 1980 atatacttta gattgattta
aaacttcatt tttaatttaa aaggatctag gtgaagatcc 2040 tttttgataa
tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 2100
accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
2160 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
caagagctac 2220 caactctttt tccgaaggta actggcttca gcagagcgca
gataccaaat actgtccttc 2280 tagtgtagcc gtagttaggc caccacttca
agaactctgt agcaccgcct acatacctcg 2340 ctctgctaat cctgttacca
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2400 tggactcaag
acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 2460
gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
2520 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
gtaagcggca 2580 gggtcggaac aggagagcgc acgagggagc ttccaggggg
aaacgcctgg tatctttata 2640 gtcctgtcgg gtttcgccac ctctgacttg
agcgtcgatt tttgtgatgc tcgtcagggg 2700 ggcggagcct atggaaaaac
gccagcaacg cggccttttt acggttcctg gccttttgct 2760 ggccttttgc
tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 2820
ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
2880 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
cgttggccga 2940 ttcattaatg cagctggcac gacaggtttc ccgactggaa
agcgggcagt gagcgcaacg 3000 caattaatgt gagttagctc actcattagg
caccccaggc tttacacttt atgcttccgg 3060 ctcgtatgtt gtgtggaatt
gtgagcggat aacaatttca cacaggaaac agctatgacc 3120 atgattacgc
caagcttgca tgcctgcagg tcgactcgac gtacgtcctc gaagagaagg 3180
gttaataaca cattttttaa catttttaac acaaatttta gttatttaaa aatttattaa
3240 aaaatttaaa ataagaagag gaactcttta aataaatcta acttacaaaa
tttatgattt 3300 ttaataagtt ttcaccaata aaaaatgtca taaaaatatg
ttaaaaagta tattatcaat 3360 attctcttta tgataaataa aaagaaaaaa
aaaataaaag ttaagtgaaa atgagattga 3420 agtgacttta ggtgtgtata
aatatatcaa ccccgccaac aatttattta atccaaatat 3480 attgaagtat
attattccat agcctttatt tatttatata tttattatat aaaagcttta 3540
tttgttctag gttgttcatg aaatattttt ttggttttat ctccgttgta agaaaatcat
3600 gtgctttgtg tcgccactca ctattgcagc tttttcatgc attggtcaga
ttgacggttg 3660 attgtatttt tgttttttat ggttttgtgt tatgacttaa
gtcttcatct ctttatctct 3720 tcatcaggtt tgatggttac ctaatatggt
ccatgggtac atgcatggtt aaattaggtg 3780 gccaactttg ttgtgaacga
tagaattttt tttatattaa gtaaactatt tttatattat 3840 gaaataataa
taaaaaaaat attttatcat tattaacaaa atcatattag ttaatttgtt 3900
aactctataa taaaagaaat actgtaacat tcacattaca tggtaacatc tttccaccct
3960 ttcatttgtt ttttgtttga tgactttttt tcttgtttaa atttatttcc
cttcttttaa 4020 atttggaata cattatcatc atatataaac taaaatacta
aaaacaggat tacacaaatg 4080 ataaataata acacaaatat ttataaatct
agctgcaata tatttaaact agctatatcg 4140 atattgtaaa ataaaactag
ctgcattgat actgataaaa aaatatcatg tgctttctgg 4200 actgatgatg
cagtatactt ttgacattgc ctttatttta tttttcagaa aagctttctt 4260
agttctgggt tcttcattat ttgtttccca tctccattgt gaattgaatc atttgcttcg
4320 tgtcacaaat acaatttagn taggtacatg cattggtcag attcacggtt
tattatgtca 4380 tgacttaagt tcatggtagt acattacctg ccacgcatgc
attatattgg ttagatttga 4440 taggcaaatt tggttgtcaa caatataaat
ataaataatg tttttatatt acgaaataac 4500 agtgatcaaa acaaacagtt
ttatctttat taacaagatt ttgtttttgt ttgatgacgt 4560 tttttaatgt
ttacgctttc ccccttcttt tgaatttaga acactttatc atcataaaat 4620
caaatactaa aaaaattaca tatttcataa ataataacac aaatattttt aaaaaatctg
4680 aaataataat gaacaatatt acatattatc acgaaaattc attaataaaa
atattatata 4740 aataaaatgt aatagtagtt atatgtagga aaaaagtact
gcacgcataa tatatacaaa 4800 aagattaaaa tgaactatta taaataataa
cactaaatta atggtgaatc atatcaaaat 4860 aatgaaaaag taaataaaat
ttgtaattaa cttctatatg tattacacac acaaataata 4920 aataatagta
aaaaaaatta tgataaatat ttaccatctc ataagatatt taaaataatg 4980
ataaaaatat agattatttt ttatgcaact agctagccaa aaagagaaca cgggtatata
5040 taaaaagagt acctttaaat tctactgtac ttcctttatt cctgacgttt
ttatatcaag 5100 tggacatacg tgaagatttt aattatcagt ctaaatattt
cattagcact taatactttt 5160 ctgttttatt cctatcctat aagtagtccc
gattctccca acattgctta ttcacacaac 5220 taactaagaa agtcttccat
agccccccaa gcggccgcgg gaattcgatt gaaatgaagt 5280 caaagcgcca
agcgcttccc cttacaattg atggaacaac atatgatgtg tctgcctggg 5340
tcaatttcca ccctggtggt gcggaaatta tagagaatta ccaaggaagg gatgccactg
5400 atgccttcat ggttatgcac tctcaagaag ccttcgacaa gctcaagcgc
atgcccaaaa 5460 tcaatcccag ttctgagttg ccaccccagg ctgcagtgaa
tgaagctcaa gaggatttcc 5520 ggaagctccg agaagagttg atcgcaactg
gcatgtttga tgcctccccc ctctggtact 5580 catacaaaat cagcaccaca
ctgggccttg gagtgctggg ttatttcctg atggttcagt 5640 atcagatgta
tttcattggg gcagtgttgc ttgggatgca ctatcaacag atgggctggc 5700
tttctcatga catttgccac caccagactt tcaagaaccg gaactggaac aacctcgtgg
5760 gactggtatt tggcaatggt ctgcaaggtt tttccgtgac atggtggaag
gacagacaca 5820 atgcacatca ttcggcaacc aatgttcaag ggcacgaccc
tgatattgac aacctccccc 5880 tcttagcctg gtctgaggat gacgtcacac
gggcgtcacc gatttcccgc aagctcattc 5940 agttccagca gtactatttc
ttggtcatct gtatcttgtt gcggttcatt tggtgtttcc 6000 agagcgtgtt
gaccgtgcgc agtttgaagg acagagataa ccaattctat cgctctcagt 6060
ataagaagga ggccattggc ctcgccctgc actggacctt gaagaccctg ttccacttat
6120 tctttatgcc cagcatcctc acatcgctgt tggtgttttt cgtttcggag
ctggttggcg 6180 gcttcggcat tgcgatcgtg gtgttcatga accactaccc
actggagaag atcggggact 6240 cagtctggga tggccatgga ttctcggttg
gccagatcca tgagaccatg aacattcggc 6300 gagggattat cacagattgg
tttttcggag gcttgaatta ccagattgag caccatttgt 6360 ggccgaccct
ccctcgccac aacctgacag cggttagcta ccaggtggaa cagctgtgcc 6420
agaagcacaa cctgccgtat cggaacccgc tgccccatga agggttggtc atcctgctgc
6480 gctatctggc ggtgttcgcc cggatggcgg agaagcaacc cgcggggaag
gctctataag 6540 gaatcactag tgaattcgc 6559 <210> SEQ ID NO 87
<211> LENGTH: 9014 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR913 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (7839)..(7839)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 87 gtacgagatc cggccggcca gatcctgcag gagatccaag cttttgatcc
atgcccttca 60 tttgccgctt attaattaat ttggtaacag tccgtactaa
tcagttactt atccttcccc 120 catcataatt aatcttggta gtctcgaatg
ccacaacact gactagtctc ttggatcata 180 agaaaaagcc aaggaacaaa
agaagacaaa acacaatgag agtatccttt gcatagcaat 240 gtctaagttc
ataaaattca aacaaaaacg caatcacaca cagtggacat cacttatcca 300
ctagctgatc aggatcgccg cgtcaagaaa aaaaaactgg accccaaaag ccatgcacaa
360 caacacgtac tcacaaaggt gtcaatcgag cagcccaaaa cattcaccaa
ctcaacccat 420 catgagccct cacatttgtt gtttctaacc caacctcaaa
ctcgtattct cttccgccac 480 ctcatttttg tttatttcaa cacccgtcaa
actgcatgcc accccgtggc caaatgtcca 540 tgcatgttaa caagacctat
gactataaat agctgcaatc tcggcccagg ttttcatcat 600 caagaaccag
ttcaatatcc tagtacaccg tattaaagaa tttaagatat actgcggccg 660
caccatggag gtggtgaatg aaatagtctc aattgggcag gaagttttac ccaaagttga
720 ttatgcccaa ctctggagtg atgccagtca ctgtgaggtg ctttacttgt
ccatcgcatt 780 tgtcatcttg aagttcactc ttggccccct tggtccaaaa
ggtcagtctc gtatgaagtt 840 tgttttcacc aattacaacc ttctcatgtc
catttattcg ttgggatcat tcctctcaat 900 ggcatatgcc atgtacacca
tcggtgttat gtctgacaac tgcgagaagg cttttgacaa 960 caacgtcttc
aggatcacca cgcagttgtt ctatttgagc aagttcctgg agtatattga 1020
ctccttctat ttgccactga tgggcaagcc tctgacctgg ttgcaattct tccatcattt
1080 gggggcaccg atggatatgt ggctgttcta taattaccga aatgaagctg
tttggatttt 1140 tgtgctgttg aatggtttca tccactggat catgtacggt
tattattgga ccagattgat 1200 caagctgaag ttccccatgc caaaatccct
gattacatca atgcagatca ttcaattcaa 1260 tgttggtttc tacattgtct
ggaagtacag gaacattccc tgttatcgcc aagatgggat 1320 gaggatgttt
ggctggttct tcaattactt ttatgttggc acagtcttgt gtttgttctt 1380
gaatttctat gtgcaaacgt atatcgtcag gaagcacaag ggagccaaaa agattcagtg
1440 agcggccgca agtatgaact aaaatgcatg taggtgtaag agctcatgga
gagcatggaa 1500 tattgtatcc gaccatgtaa cagtataata actgagctcc
atctcacttc ttctatgaat 1560 aaacaaagga tgttatgata tattaacact
ctatctatgc accttattgt tctatgataa 1620 atttcctctt attattataa
atcatctgaa tcgtgacggc ttatggaatg cttcaaatag 1680 tacaaaaaca
aatgtgtact ataagacttt ctaaacaatt ctaaccttag cattgtgaac 1740
gagacataag tgttaagaag acataacaat tataatggaa gaagtttgtc tccatttata
1800 tattatatat tacccactta tgtattatat taggatgtta aggagacata
acaattataa 1860 agagagaagt ttgtatccat ttatatatta tatactaccc
atttatatat tatacttatc 1920 cacttattta atgtctttat aaggtttgat
ccatgatatt tctaatattt tagttgatat 1980 gtatatgaaa gggtactatt
tgaactctct tactctgtat aaaggttgga tcatccttaa 2040 agtgggtcta
tttaatttta ttgcttctta cagataaaaa aaaaattatg agttggtttg 2100
ataaaatatt gaaggattta aaataataat aaataacata taatatatgt atataaattt
2160 attataatat aacatttatc tataaaaaag taaatattgt cataaatcta
tacaatcgtt 2220 tagccttgct ggacgaatct caattattta aacgagagta
aacatatttg actttttggt 2280 tatttaacaa attattattt aacactatat
gaaatttttt tttttatcag caaagaataa 2340 aattaaatta agaaggacaa
tggtgtccca atccttatac aaccaacttc cacaagaaag 2400 tcaagtcaga
gacaacaaaa aaacaagcaa aggaaatttt ttaatttgag ttgtcttgtt 2460
tgctgcataa tttatgcagt aaaacactac acataaccct tttagcagta gagcaatggt
2520 tgaccgtgtg cttagcttct tttattttat ttttttatca gcaaagaata
aataaaataa 2580 aatgagacac ttcagggatg tttcaacaag cttggcgcgc
cgttctatag tgtcacctaa 2640 atcgtatgtg tatgatacat aaggttatgt
attaattgta gccgcgttct aacgacaata 2700 tgtccatatg gtgcactctc
agtacaatct gctctgatgc cgcatagtta agccagcccc 2760 gacacccgcc
aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2820
acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
2880 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
aatgtcatga 2940 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
agaccccgta gaaaagatca 3000 aaggatcttc ttgagatcct ttttttctgc
gcgtaatctg ctgcttgcaa acaaaaaaac 3060 caccgctacc agcggtggtt
tgtttgccgg atcaagagct accaactctt tttccgaagg 3120 taactggctt
cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 3180
gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
3240 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
agacgatagt 3300 taccggataa ggcgcagcgg tcgggctgaa cggggggttc
gtgcacacag cccagcttgg 3360 agcgaacgac ctacaccgaa ctgagatacc
tacagcgtga gcattgagaa agcgccacgc 3420 ttcccgaagg gagaaaggcg
gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3480 gcacgaggga
gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3540
acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
3600 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
gctcacatgt 3660 tctttcctgc gttatcccct gattctgtgg ataaccgtat
taccgccttt gagtgagctg 3720 ataccgctcg ccgcagccga acgaccgagc
gcagcgagtc agtgagcgag gaagcggaag 3780 agcgcccaat acgcaaaccg
cctctccccg cgcgttggcc gattcattaa tgcaggttga 3840 tcagatctcg
atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc 3900
tctagaaata attttgttta actttaagaa ggagatatac ccatggaaaa gcctgaactc
3960 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
cgacctgatg 4020 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
atgtaggagg gcgtggatat 4080 gtcctgcggg taaatagctg cgccgatggt
ttctacaaag atcgttatgt ttatcggcac 4140 tttgcatcgg ccgcgctccc
gattccggaa gtgcttgaca ttggggaatt cagcgagagc 4200 ctgacctatt
gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc 4260
gaactgcccg ctgttctgca gccggtcgcg gaggctatgg atgcgatcgc tgcggccgat
4320 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
atacactaca 4380 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
atcactggca aactgtgatg 4440 gacgacaccg tcagtgcgtc cgtcgcgcag
gctctcgatg agctgatgct ttgggccgag 4500 gactgccccg aagtccggca
cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg 4560 gacaatggcc
gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa 4620
tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
4680 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
ggcgtatatg 4740 ctccgcattg gtcttgacca actctatcag agcttggttg
acggcaattt cgatgatgca 4800 gcttgggcgc agggtcgatg cgacgcaatc
gtccgatccg gagccgggac tgtcgggcgt 4860 acacaaatcg cccgcagaag
cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc 4920 gatagtggaa
accgacgccc cagcactcgt ccgagggcaa aggaatagtg aggtacagct 4980
tggatcgatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
5040 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
ttttttgctg 5100 aaaggaggaa ctatatccgg atgatcgggc gcgccgtcga
cggatccgta cgcaaaggca 5160 aagatttaaa ctcgaaaaca ttacaaaagt
ctcaaaacag aggcaaggcc atgcacaaag 5220 cacactctaa gtgcttccat
tgcctactaa gtagggtacg tacacgatca ccattcacca 5280 gtgatgatct
ttattaatat acaacacact cagagacagc ttatgttata gctagctagc 5340
ataaactatc acatcatgtg ttagtacgac aagtgacaac attgctttta acttcgcggc
5400 cttggatcct ctagaccgga tataatgagc cgtaaacaaa gatgattaag
tagtaattaa 5460 tacgtactag taaaagtggc aaaagataac gagaaagaac
caatttcttt gcattcggcc 5520 ttagcggaag gcatatataa gctttgatta
ttttatttag tgtaatgatt tcgtacaacc 5580 aaagcattta tttagtactc
tcacacttgt gtcgcggccg cgaattcact agtgattcct 5640 tatagagcct
tccccgcggg ttgcttctcc gccatccggg cgaacaccgc cagatagcgc 5700
agcaggatga ccaacccttc atggggcagc gggttccgat acggcaggtt gtgcttctgg
5760 cacagctgtt ccacctggta gctaaccgct gtcaggttgt ggcgagggag
ggtcggccac 5820 aaatggtgct caatctggta attcaagcct ccgaaaaacc
aatctgtgat aatccctcgc 5880 cgaatgttca tggtctcatg gatctggcca
accgagaatc catggccatc ccagactgag 5940 tccccgatct tctccagtgg
gtagtggttc atgaacacca cgatcgcaat gccgaagccg 6000 ccaaccagct
ccgaaacgaa aaacaccaac agcgatgtga ggatgctggg cataaagaat 6060
aagtggaaca gggtcttcaa ggtccagtgc agggcgaggc caatggcctc cttcttatac
6120 tgagagcgat agaattggtt atctctgtcc ttcaaactgc gcacggtcaa
cacgctctgg 6180 aaacaccaaa tgaaccgcaa caagatacag atgaccaaga
aatagtactg ctggaactga 6240 atgagcttgc gggaaatcgg tgacgcccgt
gtgacgtcat cctcagacca ggctaagagg 6300 gggaggttgt caatatcagg
gtcgtgccct tgaacattgg ttgccgaatg atgtgcattg 6360 tgtctgtcct
tccaccatgt cacggaaaaa ccttgcagac cattgccaaa taccagtccc 6420
acgaggttgt tccagttccg gttcttgaaa gtctggtggt ggcaaatgtc atgagaaagc
6480 cagcccatct gttgatagtg catcccaagc aacactgccc caatgaaata
catctgatac 6540 tgaaccatca ggaaataacc cagcactcca aggcccagtg
tggtgctgat tttgtatgag 6600 taccagaggg gggaggcatc aaacatgcca
gttgcgatca actcttctcg gagcttccgg 6660 aaatcctctt gagcttcatt
cactgcagcc tggggtggca actcagaact gggattgatt 6720 ttgggcatgc
gcttgagctt gtcgaaggct tcttgagagt gcataaccat gaaggcatca 6780
gtggcatccc ttccttggta attctctata atttccgcac caccagggtg gaaattgacc
6840 caggcagaca catcatatgt tgttccatca attgtaaggg gaagcgcttg
gcgctttgac 6900 ttcatttcaa tcgaattccc gcggccgctt ggggggctat
ggaagacttt cttagttagt 6960 tgtgtgaata agcaatgttg ggagaatcgg
gactacttat aggataggaa taaaacagaa 7020 aagtattaag tgctaatgaa
atatttagac tgataattaa aatcttcacg tatgtccact 7080 tgatataaaa
acgtcaggaa taaaggaagt acagtagaat ttaaaggtac tctttttata 7140
tatacccgtg ttctcttttt ggctagctag ttgcataaaa aataatctat atttttatca
7200 ttattttaaa tatcttatga gatggtaaat atttatcata atttttttta
ctattattta 7260 ttatttgtgt gtgtaataca tatagaagtt aattacaaat
tttatttact ttttcattat 7320 tttgatatga ttcaccatta atttagtgtt
attatttata atagttcatt ttaatctttt 7380 tgtatatatt atgcgtgcag
tacttttttc ctacatataa ctactattac attttattta 7440 tataatattt
ttattaatga attttcgtga taatatgtaa tattgttcat tattatttca 7500
gattttttaa aaatatttgt gttattattt atgaaatatg taattttttt agtatttgat
7560 tttatgatga taaagtgttc taaattcaaa agaaggggga aagcgtaaac
attaaaaaac 7620 gtcatcaaac aaaaacaaaa tcttgttaat aaagataaaa
ctgtttgttt tgatcactgt 7680 tatttcgtaa tataaaaaca ttatttatat
ttatattgtt gacaaccaaa tttgcctatc 7740 aaatctaacc aatataatgc
atgcgtggca ggtaatgtac taccatgaac ttaagtcatg 7800 acataataaa
ccgtgaatct gaccaatgca tgtacctanc taaattgtat ttgtgacacg 7860
aagcaaatga ttcaattcac aatggagatg ggaaacaaat aatgaagaac ccagaactaa
7920 gaaagctttt ctgaaaaata aaataaaggc aatgtcaaaa gtatactgca
tcatcagtcc 7980 agaaagcaca tgatattttt ttatcagtat caatgcagct
agttttattt tacaatatcg 8040 atatagctag tttaaatata ttgcagctag
atttataaat atttgtgtta ttatttatca 8100 tttgtgtaat cctgttttta
gtattttagt ttatatatga tgataatgta ttccaaattt 8160 aaaagaaggg
aaataaattt aaacaagaaa aaaagtcatc aaacaaaaaa caaatgaaag 8220
ggtggaaaga tgttaccatg taatgtgaat gttacagtat ttcttttatt atagagttaa
8280 caaattaact aatatgattt tgttaataat gataaaatat tttttttatt
attatttcat 8340 aatataaaaa tagtttactt aatataaaaa aaattctatc
gttcacaaca aagttggcca 8400 cctaatttaa ccatgcatgt acccatggac
catattaggt aaccatcaaa cctgatgaag 8460 agataaagag atgaagactt
aagtcataac acaaaaccat aaaaaacaaa aatacaatca 8520 accgtcaatc
tgaccaatgc atgaaaaagc tgcaatagtg agtggcgaca caaagcacat 8580
gattttctta caacggagat aaaaccaaaa aaatatttca tgaacaacct agaacaaata
8640 aagcttttat ataataaata tataaataaa taaaggctat ggaataatat
acttcaatat 8700 atttggatta aataaattgt tggcggggtt gatatattta
tacacaccta aagtcacttc 8760 aatctcattt tcacttaact tttatttttt
ttttcttttt atttatcata aagagaatat 8820 tgataatata ctttttaaca
tatttttatg acatttttta ttggtgaaaa cttattaaaa 8880 atcataaatt
ttgtaagtta gatttattta aagagttcct cttcttattt taaatttttt 8940
aataaatttt taaataacta aaatttgtgt taaaaatgtt aaaaaatgtg ttattaaccc
9000 ttctcttcga ggac 9014 <210> SEQ ID NO 88 <211>
LENGTH: 5561 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pKR767 <400> SEQUENCE: 88 catggtcaat caatgagacg
ccaacttctt aatctattga gacctgcagg tctagaaggg 60 cggatccccg
ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg 120
aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
180 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
ctgaatggcg 240 aatggcgcct gatgcggtat tttctcctta cgcatctgtg
cggtatttca caccgcatat 300 ggtgcactct cagtacaatc tgctctgatg
ccgcatagtt aagccagccc cgacacccgc 360 caacacccgc tgacgcgccc
tgacgggctt gtctgctccc ggcatccgct tacagacaag 420 ctgtgaccgt
ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 480
cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
540 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
atttgtttat 600 ttttctaaat acattcaaat atgtatccgc tcatgagaca
ataaccctga taaatgcttc 660 aataatattg aaaaaggaag agtatgagta
ttcaacattt ccgtgtcgcc cttattccct 720 tttttgcggc attttgcctt
cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 780 atgctgaaga
tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 840
agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
900 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
ggtcgccgca 960 tacactattc tcagaatgac ttggttgagt actcaccagt
cacagaaaag catcttacgg 1020 atggcatgac agtaagagaa ttatgcagtg
ctgccataac catgagtgat aacactgcgg 1080 ccaacttact tctgacaacg
atcggaggac cgaaggagct aaccgctttt ttgcacaaca 1140 tgggggatca
tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 1200
acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
1260 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
gaggcggata 1320 aagttgcagg accacttctg cgctcggccc ttccggctgg
ctggtttatt gctgataaat 1380 ctggagccgg tgagcgtggg tctcgcggta
tcattgcagc actggggcca gatggtaagc 1440 cctcccgtat cgtagttatc
tacacgacgg ggagtcaggc aactatggat gaacgaaata 1500 gacagatcgc
tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 1560
actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
1620 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
ttccactgag 1680 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
tccttttttt ctgcgcgtaa 1740 tctgctgctt gcaaacaaaa aaaccaccgc
taccagcggt ggtttgtttg ccggatcaag 1800 agctaccaac tctttttccg
aaggtaactg gcttcagcag agcgcagata ccaaatactg 1860 tccttctagt
gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1920
acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
1980 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
tgaacggggg 2040 gttcgtgcac acagcccagc ttggagcgaa cgacctacac
cgaactgaga tacctacagc 2100 gtgagctatg agaaagcgcc acgcttcccg
aagggagaaa ggcggacagg tatccggtaa 2160 gcggcagggt cggaacagga
gagcgcacga gggagcttcc agggggaaac gcctggtatc 2220 tttatagtcc
tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 2280
caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
2340 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
gtggataacc 2400 gtattaccgc ctttgagtga gctgataccg ctcgccgcag
ccgaacgacc gagcgcagcg 2460 agtcagtgag cgaggaagcg gaagagcgcc
caatacgcaa accgcctctc cccgcgcgtt 2520 ggccgattca ttaatgcagc
tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 2580 gcaacgcaat
taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 2640
ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
2700 atgaccatga ttacgccaag cttgcatgcc tgcaggctag cctaagtacg
tactcaaaat 2760 gccaacaaat aaaaaaaaag ttgctttaat aatgccaaaa
caaattaata aaacacttac 2820 aacaccggat tttttttaat taaaatgtgc
catttaggat aaatagttaa tatttttaat 2880 aattatttaa aaagccgtat
ctactaaaat gatttttatt tggttgaaaa tattaatatg 2940 tttaaatcaa
cacaatctat caaaattaaa ctaaaaaaaa aataagtgta cgtggttaac 3000
attagtacag taatataaga ggaaaatgag aaattaagaa attgaaagcg agtctaattt
3060 ttaaattatg aacctgcata tataaaagga aagaaagaat ccaggaagaa
aagaaatgaa 3120 accatgcatg gtcccctcgt catcacgagt ttctgccatt
tgcaatagaa acactgaaac 3180 acctttctct ttgtcactta attgagatgc
cgaagccacc tcacaccatg aacttcatga 3240 ggtgtagcac ccaaggcttc
catagccatg catactgaag aatgtctcaa gctcagcacc 3300 ctacttctgt
gacgtgtccc tcattcacct tcctctcttc cctataaata accacgcctc 3360
aggttctccg cttcacaact caaacattct ctccattggt ccttaaacac tcatcagtca
3420 tcaccgcggc cgcatgggaa cggaccaagg aaaaaccttc acctgggaag
agctggcggc 3480 ccataacacc aaggacgacc tactcttggc catccgcggc
agggtgtacg atgtcacaaa 3540 gttcttgagc cgccatcctg gtggagtgga
cactctcctg ctcggagctg gccgagatgt 3600 tactccggtc tttgagatgt
atcacgcgtt tggggctgca gatgccatta tgaagaagta 3660 ctatgtcggt
acactggtct cgaatgagct gcccatcttc ccggagccaa cggtgttcca 3720
caaaaccatc aagacgagag tcgagggcta ctttacggat cggaacattg atcccaagaa
3780 tagaccagag atctggggac gatacgctct tatctttgga tccttgatcg
cttcctacta 3840 cgcgcagctc tttgtgcctt tcgttgtcga acgcacatgg
cttcaggtgg tgtttgcaat 3900 catcatggga tttgcgtgcg cacaagtcgg
actcaaccct cttcatgatg cgtctcactt 3960 ttcagtgacc cacaacccca
ctgtctggaa gattctggga gccacgcacg actttttcaa 4020 cggagcatcg
tacctggtgt ggatgtacca acatatgctc ggccatcacc cctacaccaa 4080
cattgctgga gcagatcccg acgtgtcgac gtctgagccc gatgttcgtc gtatcaagcc
4140 caaccaaaag tggtttgtca accacatcaa ccagcacatg tttgttcctt
tcctgtacgg 4200 actgctggcg ttcaaggtgc gcattcagga catcaacatt
ttgtactttg tcaagaccaa 4260 tgacgctatt cgtgtcaatc ccatctcgac
atggcacact gtgatgttct ggggcggcaa 4320 ggctttcttt gtctggtatc
gcctgattgt tcccctgcag tatctgcccc tgggcaaggt 4380 gctgctcttg
ttcacggtcg cggacatggt gtcgtcttac tggctggcgc tgaccttcca 4440
ggcgaaccac gttgttgagg aagttcagtg gccgttgcct gacgagaacg ggatcatcca
4500 aaaggactgg gcagctatgc aggtcgagac tacgcaggat tacgcacacg
attcgcacct 4560 ctggaccagc atcactggca gcttgaacta ccaggctgtg
caccatctgt tccccaacgt 4620 gtcgcagcac cattatcccg atattctggc
catcatcaag aacacctgca gcgagtacaa 4680 ggttccatac cttgtcaagg
atacgttttg gcaagcattt gcttcacatt tggagcactt 4740 gcgtgttctt
ggactccgtc ccaaggaaga gtaggcggcc gcatttcgca ccaaatcaat 4800
gaaagtaata atgaaaagtc tgaataagaa tacttaggct tagatgcctt tgttacttgt
4860 gtaaaataac ttgagtcatg tacctttggc ggaaacagaa taaataaaag
gtgaaattcc 4920 aatgctctat gtataagtta gtaatactta atgtgttcta
cggttgtttc aatatcatca 4980 aactctaatt gaaactttag aaccacaaat
ctcaatcttt tcttaatgaa atgaaaaatc 5040 ttaattgtac catgtttatg
ttaaacacct tacaattggt tggagaggag gaccaaccga 5100 tgggacaaca
ttgggagaaa gagattcaat ggagatttgg ataggagaac aacattcttt 5160
ttcacttcaa tacaagatga gtgcaacact aaggatatgt atgagacttt cagaagctac
5220 gacaacatag atgagtgagg tggtgattcc tagcaagaaa gacattagag
gaagccaaaa 5280 tcgaacaagg aagacatcaa gggcaagaga caggaccatc
catctcagga aaaggagctt 5340 tgggatagtc cgagaagttg tacaagaaat
tttttggagg gtgagtgatg cattgctggt 5400 gactttaact caatcaaaat
tgagaaagaa agaaaaggga gggggctcac atgtgaatag 5460 aagggaaacg
ggagaatttt acagttttga tctaatgggc atcccagcta gtggtaacat 5520
attcaccatg tttaaccttc acgtacgtct agaggatccc c 5561 <210> SEQ
ID NO 89 <211> LENGTH: 11889 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR916 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(7810)..(7810) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 89 ggagatccaa gcttttgatc catgcccttc
atttgccgct tattaattaa tttggtaaca 60 gtccgtacta atcagttact
tatccttccc ccatcataat taatcttggt agtctcgaat 120 gccacaacac
tgactagtct cttggatcat aagaaaaagc caaggaacaa aagaagacaa 180
aacacaatga gagtatcctt tgcatagcaa tgtctaagtt cataaaattc aaacaaaaac
240 gcaatcacac acagtggaca tcacttatcc actagctgat caggatcgcc
gcgtcaagaa 300 aaaaaaactg gaccccaaaa gccatgcaca acaacacgta
ctcacaaagg tgtcaatcga 360 gcagcccaaa acattcacca actcaaccca
tcatgagccc tcacatttgt tgtttctaac 420 ccaacctcaa actcgtattc
tcttccgcca cctcattttt gtttatttca acacccgtca 480 aactgcatgc
caccccgtgg ccaaatgtcc atgcatgtta acaagaccta tgactataaa 540
tagctgcaat ctcggcccag gttttcatca tcaagaacca gttcaatatc ctagtacacc
600 gtattaaaga atttaagata tactgcggcc gcaccatgga ggtggtgaat
gaaatagtct 660 caattgggca ggaagtttta cccaaagttg attatgccca
actctggagt gatgccagtc 720 actgtgaggt gctttacttg tccatcgcat
ttgtcatctt gaagttcact cttggccccc 780 ttggtccaaa aggtcagtct
cgtatgaagt ttgttttcac caattacaac cttctcatgt 840 ccatttattc
gttgggatca ttcctctcaa tggcatatgc catgtacacc atcggtgtta 900
tgtctgacaa ctgcgagaag gcttttgaca acaacgtctt caggatcacc acgcagttgt
960 tctatttgag caagttcctg gagtatattg actccttcta tttgccactg
atgggcaagc 1020 ctctgacctg gttgcaattc ttccatcatt tgggggcacc
gatggatatg tggctgttct 1080 ataattaccg aaatgaagct gtttggattt
ttgtgctgtt gaatggtttc atccactgga 1140 tcatgtacgg ttattattgg
accagattga tcaagctgaa gttccccatg ccaaaatccc 1200 tgattacatc
aatgcagatc attcaattca atgttggttt ctacattgtc tggaagtaca 1260
ggaacattcc ctgttatcgc caagatggga tgaggatgtt tggctggttc ttcaattact
1320 tttatgttgg cacagtcttg tgtttgttct tgaatttcta tgtgcaaacg
tatatcgtca 1380 ggaagcacaa gggagccaaa aagattcagt gagcggccgc
aagtatgaac taaaatgcat 1440 gtaggtgtaa gagctcatgg agagcatgga
atattgtatc cgaccatgta acagtataat 1500 aactgagctc catctcactt
cttctatgaa taaacaaagg atgttatgat atattaacac 1560 tctatctatg
caccttattg ttctatgata aatttcctct tattattata aatcatctga 1620
atcgtgacgg cttatggaat gcttcaaata gtacaaaaac aaatgtgtac tataagactt
1680 tctaaacaat tctaacctta gcattgtgaa cgagacataa gtgttaagaa
gacataacaa 1740 ttataatgga agaagtttgt ctccatttat atattatata
ttacccactt atgtattata 1800 ttaggatgtt aaggagacat aacaattata
aagagagaag tttgtatcca tttatatatt 1860 atatactacc catttatata
ttatacttat ccacttattt aatgtcttta taaggtttga 1920 tccatgatat
ttctaatatt ttagttgata tgtatatgaa agggtactat ttgaactctc 1980
ttactctgta taaaggttgg atcatcctta aagtgggtct atttaatttt attgcttctt
2040 acagataaaa aaaaaattat gagttggttt gataaaatat tgaaggattt
aaaataataa 2100 taaataacat ataatatatg tatataaatt tattataata
taacatttat ctataaaaaa 2160 gtaaatattg tcataaatct atacaatcgt
ttagccttgc tggacgaatc tcaattattt 2220 aaacgagagt aaacatattt
gactttttgg ttatttaaca aattattatt taacactata 2280 tgaaattttt
ttttttatca gcaaagaata aaattaaatt aagaaggaca atggtgtccc 2340
aatccttata caaccaactt ccacaagaaa gtcaagtcag agacaacaaa aaaacaagca
2400 aaggaaattt tttaatttga gttgtcttgt ttgctgcata atttatgcag
taaaacacta 2460 cacataaccc ttttagcagt agagcaatgg ttgaccgtgt
gcttagcttc ttttatttta 2520 tttttttatc agcaaagaat aaataaaata
aaatgagaca cttcagggat gtttcaacaa 2580 gcttggcgcg ccgttctata
gtgtcaccta aatcgtatgt gtatgataca taaggttatg 2640 tattaattgt
agccgcgttc taacgacaat atgtccatat ggtgcactct cagtacaatc 2700
tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
2760 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
ctccgggagc 2820 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
cgagacgaaa gggcctcgtg 2880 atacgcctat ttttataggt taatgtcatg
accaaaatcc cttaacgtga gttttcgttc 2940 cactgagcgt cagaccccgt
agaaaagatc aaaggatctt cttgagatcc tttttttctg 3000 cgcgtaatct
gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 3060
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
3120 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
tgtagcaccg 3180 cctacatacc tcgctctgct aatcctgtta ccagtggctg
ctgccagtgg cgataagtcg 3240 tgtcttaccg ggttggactc aagacgatag
ttaccggata aggcgcagcg gtcgggctga 3300 acggggggtt cgtgcacaca
gcccagcttg gagcgaacga cctacaccga actgagatac 3360 ctacagcgtg
agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3420
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
3480 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
atttttgtga 3540 tgctcgtcag gggggcggag cctatggaaa aacgccagca
acgcggcctt tttacggttc 3600 ctggcctttt gctggccttt tgctcacatg
ttctttcctg cgttatcccc tgattctgtg 3660 gataaccgta ttaccgcctt
tgagtgagct gataccgctc gccgcagccg aacgaccgag 3720 cgcagcgagt
cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 3780
gcgcgttggc cgattcatta atgcaggttg atcagatctc gatcccgcga aattaatacg
3840 actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
aactttaaga 3900 aggagatata cccatggaaa agcctgaact caccgcgacg
tctgtcgaga agtttctgat 3960 cgaaaagttc gacagcgtct ccgacctgat
gcagctctcg gagggcgaag aatctcgtgc 4020 tttcagcttc gatgtaggag
ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg 4080 tttctacaaa
gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga 4140
agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
4200 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
agccggtcgc 4260 ggaggctatg gatgcgatcg ctgcggccga tcttagccag
acgagcgggt tcggcccatt 4320 cggaccgcaa ggaatcggtc aatacactac
atggcgtgat ttcatatgcg cgattgctga 4380 tccccatgtg tatcactggc
aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 4440 ggctctcgat
gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca 4500
cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
4560 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
tcttctggag 4620 gccgtggttg gcttgtatgg agcagcagac gcgctacttc
gagcggaggc atccggagct 4680 tgcaggatcg ccgcggctcc gggcgtatat
gctccgcatt ggtcttgacc aactctatca 4740 gagcttggtt gacggcaatt
tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat 4800 cgtccgatcc
ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt 4860
ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
4920 tccgagggca aaggaatagt gaggtacagc ttggatcgat ccggctgcta
acaaagcccg 4980 aaaggaagct gagttggctg ctgccaccgc tgagcaataa
ctagcataac cccttggggc 5040 ctctaaacgg gtcttgaggg gttttttgct
gaaaggagga actatatccg gatgatcggg 5100 cgcgccgtcg acggatccgt
acgcaaaggc aaagatttaa actcgaaaac attacaaaag 5160 tctcaaaaca
gaggcaaggc catgcacaaa gcacactcta agtgcttcca ttgcctacta 5220
agtagggtac gtacacgatc accattcacc agtgatgatc tttattaata tacaacacac
5280 tcagagacag cttatgttat agctagctag cataaactat cacatcatgt
gttagtacga 5340 caagtgacaa cattgctttt aacttcgcgg ccttggatcc
tctagaccgg atataatgag 5400 ccgtaaacaa agatgattaa gtagtaatta
atacgtacta gtaaaagtgg caaaagataa 5460 cgagaaagaa ccaatttctt
tgcattcggc cttagcggaa ggcatatata agctttgatt 5520 attttattta
gtgtaatgat ttcgtacaac caaagcattt atttagtact ctcacacttg 5580
tgtcgcggcc gcgaattcac tagtgattcc ttatagagcc ttccccgcgg gttgcttctc
5640 cgccatccgg gcgaacaccg ccagatagcg cagcaggatg accaaccctt
catggggcag 5700 cgggttccga tacggcaggt tgtgcttctg gcacagctgt
tccacctggt agctaaccgc 5760 tgtcaggttg tggcgaggga gggtcggcca
caaatggtgc tcaatctggt aattcaagcc 5820 tccgaaaaac caatctgtga
taatccctcg ccgaatgttc atggtctcat ggatctggcc 5880 aaccgagaat
ccatggccat cccagactga gtccccgatc ttctccagtg ggtagtggtt 5940
catgaacacc acgatcgcaa tgccgaagcc gccaaccagc tccgaaacga aaaacaccaa
6000 cagcgatgtg aggatgctgg gcataaagaa taagtggaac agggtcttca
aggtccagtg 6060 cagggcgagg ccaatggcct ccttcttata ctgagagcga
tagaattggt tatctctgtc 6120 cttcaaactg cgcacggtca acacgctctg
gaaacaccaa atgaaccgca acaagataca 6180 gatgaccaag aaatagtact
gctggaactg aatgagcttg cgggaaatcg gtgacgcccg 6240 tgtgacgtca
tcctcagacc aggctaagag ggggaggttg tcaatatcag ggtcgtgccc 6300
ttgaacattg gttgccgaat gatgtgcatt gtgtctgtcc ttccaccatg tcacggaaaa
6360 accttgcaga ccattgccaa ataccagtcc cacgaggttg ttccagttcc
ggttcttgaa 6420 agtctggtgg tggcaaatgt catgagaaag ccagcccatc
tgttgatagt gcatcccaag 6480 caacactgcc ccaatgaaat acatctgata
ctgaaccatc aggaaataac ccagcactcc 6540 aaggcccagt gtggtgctga
ttttgtatga gtaccagagg ggggaggcat caaacatgcc 6600 agttgcgatc
aactcttctc ggagcttccg gaaatcctct tgagcttcat tcactgcagc 6660
ctggggtggc aactcagaac tgggattgat tttgggcatg cgcttgagct tgtcgaaggc
6720 ttcttgagag tgcataacca tgaaggcatc agtggcatcc cttccttggt
aattctctat 6780 aatttccgca ccaccagggt ggaaattgac ccaggcagac
acatcatatg ttgttccatc 6840 aattgtaagg ggaagcgctt ggcgctttga
cttcatttca atcgaattcc cgcggccgct 6900 tggggggcta tggaagactt
tcttagttag ttgtgtgaat aagcaatgtt gggagaatcg 6960 ggactactta
taggatagga ataaaacaga aaagtattaa gtgctaatga aatatttaga 7020
ctgataatta aaatcttcac gtatgtccac ttgatataaa aacgtcagga ataaaggaag
7080 tacagtagaa tttaaaggta ctctttttat atatacccgt gttctctttt
tggctagcta 7140 gttgcataaa aaataatcta tatttttatc attattttaa
atatcttatg agatggtaaa 7200 tatttatcat aatttttttt actattattt
attatttgtg tgtgtaatac atatagaagt 7260 taattacaaa ttttatttac
tttttcatta ttttgatatg attcaccatt aatttagtgt 7320 tattatttat
aatagttcat tttaatcttt ttgtatatat tatgcgtgca gtactttttt 7380
cctacatata actactatta cattttattt atataatatt tttattaatg aattttcgtg
7440 ataatatgta atattgttca ttattatttc agatttttta aaaatatttg
tgttattatt 7500 tatgaaatat gtaatttttt tagtatttga ttttatgatg
ataaagtgtt ctaaattcaa 7560 aagaaggggg aaagcgtaaa cattaaaaaa
cgtcatcaaa caaaaacaaa atcttgttaa 7620 taaagataaa actgtttgtt
ttgatcactg ttatttcgta atataaaaac attatttata 7680 tttatattgt
tgacaaccaa atttgcctat caaatctaac caatataatg catgcgtggc 7740
aggtaatgta ctaccatgaa cttaagtcat gacataataa accgtgaatc tgaccaatgc
7800 atgtacctan ctaaattgta tttgtgacac gaagcaaatg attcaattca
caatggagat 7860 gggaaacaaa taatgaagaa cccagaacta agaaagcttt
tctgaaaaat aaaataaagg 7920 caatgtcaaa agtatactgc atcatcagtc
cagaaagcac atgatatttt tttatcagta 7980 tcaatgcagc tagttttatt
ttacaatatc gatatagcta gtttaaatat attgcagcta 8040 gatttataaa
tatttgtgtt attatttatc atttgtgtaa tcctgttttt agtattttag 8100
tttatatatg atgataatgt attccaaatt taaaagaagg gaaataaatt taaacaagaa
8160 aaaaagtcat caaacaaaaa acaaatgaaa gggtggaaag atgttaccat
gtaatgtgaa 8220 tgttacagta tttcttttat tatagagtta acaaattaac
taatatgatt ttgttaataa 8280 tgataaaata ttttttttat tattatttca
taatataaaa atagtttact taatataaaa 8340 aaaattctat cgttcacaac
aaagttggcc acctaattta accatgcatg tacccatgga 8400 ccatattagg
taaccatcaa acctgatgaa gagataaaga gatgaagact taagtcataa 8460
cacaaaacca taaaaaacaa aaatacaatc aaccgtcaat ctgaccaatg catgaaaaag
8520 ctgcaatagt gagtggcgac acaaagcaca tgattttctt acaacggaga
taaaaccaaa 8580 aaaatatttc atgaacaacc tagaacaaat aaagctttta
tataataaat atataaataa 8640 ataaaggcta tggaataata tacttcaata
tatttggatt aaataaattg ttggcggggt 8700 tgatatattt atacacacct
aaagtcactt caatctcatt ttcacttaac ttttattttt 8760 tttttctttt
tatttatcat aaagagaata ttgataatat actttttaac atatttttat 8820
gacatttttt attggtgaaa acttattaaa aatcataaat tttgtaagtt agatttattt
8880 aaagagttcc tcttcttatt ttaaattttt taataaattt ttaaataact
aaaatttgtg 8940 ttaaaaatgt taaaaaatgt gttattaacc cttctcttcg
aggacgtacg agatccggcc 9000 ggccagatcc tgcaggtctc aatagattaa
gaagttggcg tctcattgat tgaccatggg 9060 ggatcctcta gacgtacgtg
aaggttaaac atggtgaata tgttaccact agctgggatg 9120 cccattagat
caaaactgta aaattctccc gtttcccttc tattcacatg tgagccccct 9180
cccttttctt tctttctcaa ttttgattga gttaaagtca ccagcaatgc atcactcacc
9240 ctccaaaaaa tttcttgtac aacttctcgg actatcccaa agctcctttt
cctgagatgg 9300 atggtcctgt ctcttgccct tgatgtcttc cttgttcgat
tttggcttcc tctaatgtct 9360 ttcttgctag gaatcaccac ctcactcatc
tatgttgtcg tagcttctga aagtctcata 9420 catatcctta gtgttgcact
catcttgtat tgaagtgaaa aagaatgttg ttctcctatc 9480 caaatctcca
ttgaatctct ttctcccaat gttgtcccat cggttggtcc tcctctccaa 9540
ccaattgtaa ggtgtttaac ataaacatgg tacaattaag atttttcatt tcattaagaa
9600 aagattgaga tttgtggttc taaagtttca attagagttt gatgatattg
aaacaaccgt 9660 agaacacatt aagtattact aacttataca tagagcattg
gaatttcacc ttttatttat 9720 tctgtttccg ccaaaggtac atgactcaag
ttattttaca caagtaacaa aggcatctaa 9780 gcctaagtat tcttattcag
acttttcatt attactttca ttgatttggt gcgaaatgcg 9840 gccgcctact
cttccttggg acggagtcca agaacacgca agtgctccaa atgtgaagca 9900
aatgcttgcc aaaacgtatc cttgacaagg tatggaacct tgtactcgct gcaggtgttc
9960 ttgatgatgg ccagaatatc gggataatgg tgctgcgaca cgttggggaa
cagatggtgc 10020 acagcctggt agttcaagct gccagtgatg ctggtccaga
ggtgcgaatc gtgtgcgtaa 10080 tcctgcgtag tctcgacctg catagctgcc
cagtcctttt ggatgatccc gttctcgtca 10140 ggcaacggcc actgaacttc
ctcaacaacg tggttcgcct ggaaggtcag cgccagccag 10200 taagacgaca
ccatgtccgc gaccgtgaac aagagcagca ccttgcccag gggcagatac 10260
tgcaggggaa caatcaggcg ataccagaca aagaaagcct tgccgcccca gaacatcaca
10320 gtgtgccatg tcgagatggg attgacacga atagcgtcat tggtcttgac
aaagtacaaa 10380 atgttgatgt cctgaatgcg caccttgaac gccagcagtc
cgtacaggaa aggaacaaac 10440 atgtgctggt tgatgtggtt gacaaaccac
ttttggttgg gcttgatacg acgaacatcg 10500 ggctcagacg tcgacacgtc
gggatctgct ccagcaatgt tggtgtaggg gtgatggccg 10560 agcatatgtt
ggtacatcca caccaggtac gatgctccgt tgaaaaagtc gtgcgtggct 10620
cccagaatct tccagacagt ggggttgtgg gtcactgaaa agtgagacgc atcatgaaga
10680 gggttgagtc cgacttgtgc gcacgcaaat cccatgatga ttgcaaacac
cacctgaagc 10740 catgtgcgtt cgacaacgaa aggcacaaag agctgcgcgt
agtaggaagc gatcaaggat 10800 ccaaagataa gagcgtatcg tccccagatc
tctggtctat tcttgggatc aatgttccga 10860 tccgtaaagt agccctcgac
tctcgtcttg atggttttgt ggaacaccgt tggctccggg 10920 aagatgggca
gctcattcga gaccagtgta ccgacatagt acttcttcat aatggcatct 10980
gcagccccaa acgcgtgata catctcaaag accggagtaa catctcggcc agctccgagc
11040 aggagagtgt ccactccacc aggatggcgg ctcaagaact ttgtgacatc
gtacaccctg 11100 ccgcggatgg ccaagagtag gtcgtccttg gtgttatggg
ccgccagctc ttcccaggtg 11160 aaggtttttc cttggtccgt tcccatgcgg
ccgcggtgat gactgatgag tgtttaagga 11220 ccaatggaga gaatgtttga
gttgtgaagc ggagaacctg aggcgtggtt atttataggg 11280 aagagaggaa
ggtgaatgag ggacacgtca cagaagtagg gtgctgagct tgagacattc 11340
ttcagtatgc atggctatgg aagccttggg tgctacacct catgaagttc atggtgtgag
11400 gtggcttcgg catctcaatt aagtgacaaa gagaaaggtg tttcagtgtt
tctattgcaa 11460 atggcagaaa ctcgtgatga cgaggggacc atgcatggtt
tcatttcttt tcttcctgga 11520 ttctttcttt ccttttatat atgcaggttc
ataatttaaa aattagactc gctttcaatt 11580 tcttaatttc tcattttcct
cttatattac tgtactaatg ttaaccacgt acacttattt 11640 tttttttagt
ttaattttga tagattgtgt tgatttaaac atattaatat tttcaaccaa 11700
ataaaaatca ttttagtaga tacggctttt taaataatta ttaaaaatat taactattta
11760 tcctaaatgg cacattttaa ttaaaaaaaa tccggtgttg taagtgtttt
attaatttgt 11820 tttggcatta ttaaagcaac ttttttttta tttgttggca
ttttgagtac gtacttaggc 11880 tagcctgca 11889 <210> SEQ ID NO
90 <211> LENGTH: 5661 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR974 <400> SEQUENCE: 90
gtacgtctag aggatccccc atggtcaatc aatgagacgc caacttctta atctattgag
60 acctgcaggt ctagaagggc ggatccccgg gtaccgagct cgaattcact
ggccgtcgtt 120 ttacaacgtc gtgactggga aaaccctggc gttacccaac
ttaatcgcct tgcagcacat 180 ccccctttcg ccagctggcg taatagcgaa
gaggcccgca ccgatcgccc ttcccaacag 240 ttgcgcagcc tgaatggcga
atggcgcctg atgcggtatt ttctccttac gcatctgtgc 300 ggtatttcac
accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 360
agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
420 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
gaggttttca 480 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
tacgcctatt tttataggtt 540 aatgtcatga taataatggt ttcttagacg
tcaggtggca cttttcgggg aaatgtgcgc 600 ggaaccccta tttgtttatt
tttctaaata cattcaaata tgtatccgct catgagacaa 660 taaccctgat
aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 720
cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
780 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
ttacatcgaa 840 ctggatctca acagcggtaa gatccttgag agttttcgcc
ccgaagaacg ttttccaatg 900 atgagcactt ttaaagttct gctatgtggc
gcggtattat cccgtattga cgccgggcaa 960 gagcaactcg gtcgccgcat
acactattct cagaatgact tggttgagta ctcaccagtc 1020 acagaaaagc
atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 1080
atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
1140 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
ggaaccggag 1200 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
tgcctgtagc aatggcaaca 1260 acgttgcgca aactattaac tggcgaacta
cttactctag cttcccggca acaattaata 1320 gactggatgg aggcggataa
agttgcagga ccacttctgc gctcggccct tccggctggc 1380 tggtttattg
ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 1440
ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
1500 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
taagcattgg 1560 taactgtcag accaagttta ctcatatata ctttagattg
atttaaaact tcatttttaa 1620 tttaaaagga tctaggtgaa gatccttttt
gataatctca tgaccaaaat cccttaacgt 1680 gagttttcgt tccactgagc
gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 1740 cctttttttc
tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 1800
gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
1860 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
cttcaagaac 1920 tctgtagcac cgcctacata cctcgctctg ctaatcctgt
taccagtggc tgctgccagt 1980 ggcgataagt cgtgtcttac cgggttggac
tcaagacgat agttaccgga taaggcgcag 2040 cggtcgggct gaacgggggg
ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2100 gaactgagat
acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 2160
gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
2220 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
acttgagcgt 2280 cgatttttgt gatgctcgtc aggggggcgg agcctatgga
aaaacgccag caacgcggcc 2340 tttttacggt tcctggcctt ttgctggcct
tttgctcaca tgttctttcc tgcgttatcc 2400 cctgattctg tggataaccg
tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 2460 cgaacgaccg
agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 2520
ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
2580 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
ttaggcaccc 2640 caggctttac actttatgct tccggctcgt atgttgtgtg
gaattgtgag cggataacaa 2700 tttcacacag gaaacagcta tgaccatgat
tacgccaagc ttgcatgcct gcaggctagc 2760 ctaagtacgt actcaaaatg
ccaacaaata aaaaaaaagt tgctttaata atgccaaaac 2820 aaattaataa
aacacttaca acaccggatt ttttttaatt aaaatgtgcc atttaggata 2880
aatagttaat atttttaata attatttaaa aagccgtatc tactaaaatg atttttattt
2940 ggttgaaaat attaatatgt ttaaatcaac acaatctatc aaaattaaac
taaaaaaaaa 3000 ataagtgtac gtggttaaca ttagtacagt aatataagag
gaaaatgaga aattaagaaa 3060 ttgaaagcga gtctaatttt taaattatga
acctgcatat ataaaaggaa agaaagaatc 3120 caggaagaaa agaaatgaaa
ccatgcatgg tcccctcgtc atcacgagtt tctgccattt 3180 gcaatagaaa
cactgaaaca cctttctctt tgtcacttaa ttgagatgcc gaagccacct 3240
cacaccatga acttcatgag gtgtagcacc caaggcttcc atagccatgc atactgaaga
3300 atgtctcaag ctcagcaccc tacttctgtg acgtgtccct cattcacctt
cctctcttcc 3360 ctataaataa ccacgcctca ggttctccgc ttcacaactc
aaacattctc tccattggtc 3420 cttaaacact catcagtcat caccgcggcc
gccaattcat ggccccgcag acggagctcc 3480 gccagcgcca cgccgccgtc
gccgagacgc cggtggccgg caagaaggcc tttacatggc 3540 aggaggtcgc
gcagcacaac acggcggcct cggcctggat cattatccgc ggcaaggtct 3600
acgacgtgac cgagtgggcc aacaagcacc ccggcggccg cgagatggtg ctgctgcacg
3660 ccggtcgcga ggccaccgac acgttcgact cgtaccaccc gttcagcgac
aaggccgagt 3720 cgatcttgaa caagtatgag attggcacgt tcacgggccc
gtccgagttt ccgaccttca 3780 agccggacac gggcttctac aaggagtgcc
gcaagcgcgt tggcgagtac ttcaagaaga 3840 acaacctcca tccgcaggac
ggcttcccgg gcctctggcg catgatggtc gtgtttgcgg 3900 tcgccggcct
cgccttgtac ggcatgcact tttcgactat ctttgcgctg cagctcgcgg 3960
ccgcggcgct ctttggcgtc tgccaggcgc tgccgctgct ccacgtcatg cacgactcgt
4020 cgcacgcgtc gtacaccaac atgccgttct tccattacgt cgtcggccgc
tttgccatgg 4080 actggtttgc cggcggctcg atggtgtcat ggctcaacca
gcacgtcgtg ggccaccaca 4140 tctacacgaa cgtcgcgggc tcggacccgg
atcttccggt caacatggac ggcgacatcc 4200 gccgcatcgt gaaccgccag
gtgttccagc ccatgtacgc attccagcac atctaccttc 4260 cgccgctcta
tggcgtgctt ggcctcaagt tccgcatcca ggacttcacc gacacgttcg 4320
gctcgcacac gaacggcccg atccgcgtca acccgcacgc gctctcgacg tggatggcca
4380 tgatcagctc caagtcgttc tgggccttct accgcgtgta ccttccgctt
gccgtgctcc 4440 agatgcccat caagacgtac cttgcgatct tcttcctcgc
cgagtttgtc acgggctggt 4500 acctcgcgtt caacttccaa gtaagccatg
tctcgaccga gtgcggctac ccatgcggcg 4560 acgaggccaa gatggcgctc
caggacgagt gggcagtctc gcaggtcaag acgtcggtcg 4620 actacgccca
tggctcgtgg atgacgacgt tccttgccgg cgcgctcaac taccaggtcg 4680
tgcaccactt gttccccagc gtgtcgcagt accactaccc ggcgatcgcg cccatcatcg
4740 tcgacgtctg caaggagtac aacatcaagt acgccatctt gccggacttt
acggcggcgt 4800 tcgttgccca cttgaagcac ctccgcaaca tgggccagca
gggcatcgcc gccacgatcc 4860 acatgggcta actcgagctc agctagatcg
cggccgcatt tcgcaccaaa tcaatgaaag 4920 taataatgaa aagtctgaat
aagaatactt aggcttagat gcctttgtta cttgtgtaaa 4980 ataacttgag
tcatgtacct ttggcggaaa cagaataaat aaaaggtgaa attccaatgc 5040
tctatgtata agttagtaat acttaatgtg ttctacggtt gtttcaatat catcaaactc
5100 taattgaaac tttagaacca caaatctcaa tcttttctta atgaaatgaa
aaatcttaat 5160 tgtaccatgt ttatgttaaa caccttacaa ttaattggtt
ggagaggagg accaaccgat 5220 gggacaacat tgggagaaag agattcaatg
gagatttgga taggagaaca acattctttt 5280 tcacttcaat acaagatgag
tgcaacacta aggatatgta tgagactttc agaagctacg 5340 acaacataga
tgagtgaggt ggtgattcct agcaagaaag acattagagg aagccaaaat 5400
cgaacaagga agacatcaag ggcaagagac aggaccatcc atctcaggaa aaggagcttt
5460 gggatagtcc gagaagttgt acaagaaatt ttttggaggg tgagtgatgc
attgctggtg 5520 actttaactc aatcaaaatt gagaaagaaa gaaaagggag
ggggctcaca tgtgaataga 5580 agggaaacgg gagaatttta cagttttgat
ctaatgggca tcccagctag tggtaacata 5640 ttcaccatgt ttaaccttca c 5661
<210> SEQ ID NO 91 <211> LENGTH: 5578 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR1032 <400>
SEQUENCE: 91 ggccgcattt cgcaccaaat caatgaaagt aataatgaaa agtctgaata
agaatactta 60 ggcttagatg cctttgttac ttgtgtaaaa taacttgagt
catgtacctt tggcggaaac 120 agaataaata aaaggtgaaa ttccaatgct
ctatgtataa gttagtaata cttaatgtgt 180 tctacggttg tttcaatatc
atcaaactct aattgaaact ttagaaccac aaatctcaat 240 cttttcttaa
tgaaatgaaa aatcttaatt gtaccatgtt tatgttaaac accttacaat 300
taattggttg gagaggagga ccaaccgatg ggacaacatt gggagaaaga gattcaatgg
360 agatttggat aggagaacaa cattcttttt cacttcaata caagatgagt
gcaacactaa 420 ggatatgtat gagactttca gaagctacga caacatagat
gagtgaggtg gtgattccta 480 gcaagaaaga cattagagga agccaaaatc
gaacaaggaa gacatcaagg gcaagagaca 540 ggaccatcca tctcaggaaa
aggagctttg ggatagtccg agaagttgta caagaaattt 600 tttggagggt
gagtgatgca ttgctggtga ctttaactca atcaaaattg agaaagaaag 660
aaaagggagg gggctcacat gtgaatagaa gggaaacggg agaattttac agttttgatc
720 taatgggcat cccagctagt ggtaacatat tcaccatgtt taaccttcac
gtacgtctag 780 aggatccccc atggtcaatc aatgagacgc caacttctta
atctattgag acctgcaggt 840 ctagaagggc ggatccccgg gtaccgagct
cgaattcact ggccgtcgtt ttacaacgtc 900 gtgactggga aaaccctggc
gttacccaac ttaatcgcct tgcagcacat ccccctttcg 960 ccagctggcg
taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 1020
tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
1080 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
agccagcccc 1140 gacacccgcc aacacccgct gacgcgccct gacgggcttg
tctgctcccg gcatccgctt 1200 acagacaagc tgtgaccgtc tccgggagct
gcatgtgtca gaggttttca ccgtcatcac 1260 cgaaacgcgc gagacgaaag
ggcctcgtga tacgcctatt tttataggtt aatgtcatga 1320 taataatggt
ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 1380
tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
1440 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
cgtgtcgccc 1500 ttattccctt ttttgcggca ttttgccttc ctgtttttgc
tcacccagaa acgctggtga 1560 aagtaaaaga tgctgaagat cagttgggtg
cacgagtggg ttacatcgaa ctggatctca 1620 acagcggtaa gatccttgag
agttttcgcc ccgaagaacg ttttccaatg atgagcactt 1680 ttaaagttct
gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 1740
gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
1800 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
atgagtgata 1860 acactgcggc caacttactt ctgacaacga tcggaggacc
gaaggagcta accgcttttt 1920 tgcacaacat gggggatcat gtaactcgcc
ttgatcgttg ggaaccggag ctgaatgaag 1980 ccataccaaa cgacgagcgt
gacaccacga tgcctgtagc aatggcaaca acgttgcgca 2040 aactattaac
tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 2100
aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
2160 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
ctggggccag 2220 atggtaagcc ctcccgtatc gtagttatct acacgacggg
gagtcaggca actatggatg 2280 aacgaaatag acagatcgct gagataggtg
cctcactgat taagcattgg taactgtcag 2340 accaagttta ctcatatata
ctttagattg atttaaaact tcatttttaa tttaaaagga 2400 tctaggtgaa
gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 2460
tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
2520 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
gtttgtttgc 2580 cggatcaaga gctaccaact ctttttccga aggtaactgg
cttcagcaga gcgcagatac 2640 caaatactgt ccttctagtg tagccgtagt
taggccacca cttcaagaac tctgtagcac 2700 cgcctacata cctcgctctg
ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2760 cgtgtcttac
cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2820
gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
2880 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
gcggacaggt 2940 atccggtaag cggcagggtc ggaacaggag agcgcacgag
ggagcttcca gggggaaacg 3000 cctggtatct ttatagtcct gtcgggtttc
gccacctctg acttgagcgt cgatttttgt 3060 gatgctcgtc aggggggcgg
agcctatgga aaaacgccag caacgcggcc tttttacggt 3120 tcctggcctt
ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 3180
tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
3240 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
ccgcctctcc 3300 ccgcgcgttg gccgattcat taatgcagct ggcacgacag
gtttcccgac tggaaagcgg 3360 gcagtgagcg caacgcaatt aatgtgagtt
agctcactca ttaggcaccc caggctttac 3420 actttatgct tccggctcgt
atgttgtgtg gaattgtgag cggataacaa tttcacacag 3480 gaaacagcta
tgaccatgat tacgccaagc ttgcatgcct gcaggctagc ctaagtacgt 3540
actcaaaatg ccaacaaata aaaaaaaagt tgctttaata atgccaaaac aaattaataa
3600 aacacttaca acaccggatt ttttttaatt aaaatgtgcc atttaggata
aatagttaat 3660 atttttaata attatttaaa aagccgtatc tactaaaatg
atttttattt ggttgaaaat 3720 attaatatgt ttaaatcaac acaatctatc
aaaattaaac taaaaaaaaa ataagtgtac 3780 gtggttaaca ttagtacagt
aatataagag gaaaatgaga aattaagaaa ttgaaagcga 3840 gtctaatttt
taaattatga acctgcatat ataaaaggaa agaaagaatc caggaagaaa 3900
agaaatgaaa ccatgcatgg tcccctcgtc atcacgagtt tctgccattt gcaatagaaa
3960 cactgaaaca cctttctctt tgtcacttaa ttgagatgcc gaagccacct
cacaccatga 4020 acttcatgag gtgtagcacc caaggcttcc atagccatgc
atactgaaga atgtctcaag 4080 ctcagcaccc tacttctgtg acgtgtccct
cattcacctt cctctcttcc ctataaataa 4140 ccacgcctca ggttctccgc
ttcacaactc aaacattctc tccattggtc cttaaacact 4200 catcagtcat
caccgcggcc gcaaaccatg gctctcagtc ttaccacaga acagctgtta 4260
gaacgccctg atttggttgc gattgatggc atcctctacg accttgaagg gcttgccaaa
4320 gttcatccag gaggagattt gattctcgct tctggtgcct ctgatgcctc
ccctctcttt 4380 tattcaatgc atccatacgt caaaccggag aattccaaat
tgcttcaaca gttcgtccga 4440 gggaagcatg accgcacctc gaaggacatt
gtctacacgt atgattctcc cttcgcacaa 4500 gacgttaagc ggacaatgcg
cgaggtgatg aaagggagga actggtacgc aacccctggc 4560 ttctggctgc
gcaccgttgg gatcatcgcc gtgacggcct tttgcgagtg gcactgggct 4620
accacgggga tggtgctgtg gggcctgttg actggattca tgcacatgca gatcggctta
4680 tccatccagc atgatgcgtc ccacggggcc atcagcaaga agccttgggt
caacgccctc 4740 ttcgcctacg gcattgacgt catcggatcg tcccggtgga
tttggctgca gtcgcacatc 4800 atgcggcacc acacctacac caaccagcac
ggcctcgacc tggatgcgga gtcggcagag 4860 ccgttcctgg tgttccacaa
ctaccccgcc gcaaacaccg cccgaaagtg gttccaccgc 4920 ttccaagctt
ggtacatgta ccttgtgctg ggggcatacg gggtatcgct ggtgtacaac 4980
ccgctctaca ttttccggat gcagcacaat gacaccatcc cagagtctgt cacggccatg
5040 cgggaaaatg gctttctgcg gcgctaccgc acacttgcat tcgtgatgcg
agctttcttc 5100 atcttccgga ccgcattctt gccctggtac ctcactggga
cctcattgct gatcaccatt 5160 cctctggtgc ccaccgcaac tggtgccttc
ttgacgttct tcttcatttt gtcccacaat 5220 tttgatggct ccgaacggat
ccccgacaag aactgcaagg ttaagagatc tgagaaggac 5280 gttgaggctg
accaaattga ctggtatcgg gcgcaggtgg agacgtcctc cacatacggt 5340
ggccccatcg ccatgttctt cactggcggt ctcaatttcc agatcgagca ccacctcttt
5400 ccccggatgt cgtcttggca ctaccccttc gtccagcagg cggtccggga
gtgttgcgaa 5460 cgccatggag tgcgatatgt tttctaccct accatcgtcg
gcaacatcat ctccaccctg 5520 aagtacatgc ataaggtggg tgtcgtccac
tgcgtgaagg acgcacagga ttcctaag 5578 <210> SEQ ID NO 92
<211> LENGTH: 11907 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR1037 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (7810)..(7810)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 92 ggagatccaa gcttttgatc catgcccttc atttgccgct tattaattaa
tttggtaaca 60 gtccgtacta atcagttact tatccttccc ccatcataat
taatcttggt agtctcgaat 120 gccacaacac tgactagtct cttggatcat
aagaaaaagc caaggaacaa aagaagacaa 180 aacacaatga gagtatcctt
tgcatagcaa tgtctaagtt cataaaattc aaacaaaaac 240 gcaatcacac
acagtggaca tcacttatcc actagctgat caggatcgcc gcgtcaagaa 300
aaaaaaactg gaccccaaaa gccatgcaca acaacacgta ctcacaaagg tgtcaatcga
360 gcagcccaaa acattcacca actcaaccca tcatgagccc tcacatttgt
tgtttctaac 420 ccaacctcaa actcgtattc tcttccgcca cctcattttt
gtttatttca acacccgtca 480 aactgcatgc caccccgtgg ccaaatgtcc
atgcatgtta acaagaccta tgactataaa 540 tagctgcaat ctcggcccag
gttttcatca tcaagaacca gttcaatatc ctagtacacc 600 gtattaaaga
atttaagata tactgcggcc gcaccatgga ggtggtgaat gaaatagtct 660
caattgggca ggaagtttta cccaaagttg attatgccca actctggagt gatgccagtc
720 actgtgaggt gctttacttg tccatcgcat ttgtcatctt gaagttcact
cttggccccc 780 ttggtccaaa aggtcagtct cgtatgaagt ttgttttcac
caattacaac cttctcatgt 840 ccatttattc gttgggatca ttcctctcaa
tggcatatgc catgtacacc atcggtgtta 900 tgtctgacaa ctgcgagaag
gcttttgaca acaacgtctt caggatcacc acgcagttgt 960 tctatttgag
caagttcctg gagtatattg actccttcta tttgccactg atgggcaagc 1020
ctctgacctg gttgcaattc ttccatcatt tgggggcacc gatggatatg tggctgttct
1080 ataattaccg aaatgaagct gtttggattt ttgtgctgtt gaatggtttc
atccactgga 1140 tcatgtacgg ttattattgg accagattga tcaagctgaa
gttccccatg ccaaaatccc 1200 tgattacatc aatgcagatc attcaattca
atgttggttt ctacattgtc tggaagtaca 1260 ggaacattcc ctgttatcgc
caagatggga tgaggatgtt tggctggttc ttcaattact 1320 tttatgttgg
cacagtcttg tgtttgttct tgaatttcta tgtgcaaacg tatatcgtca 1380
ggaagcacaa gggagccaaa aagattcagt gagcggccgc aagtatgaac taaaatgcat
1440 gtaggtgtaa gagctcatgg agagcatgga atattgtatc cgaccatgta
acagtataat 1500 aactgagctc catctcactt cttctatgaa taaacaaagg
atgttatgat atattaacac 1560 tctatctatg caccttattg ttctatgata
aatttcctct tattattata aatcatctga 1620 atcgtgacgg cttatggaat
gcttcaaata gtacaaaaac aaatgtgtac tataagactt 1680 tctaaacaat
tctaacctta gcattgtgaa cgagacataa gtgttaagaa gacataacaa 1740
ttataatgga agaagtttgt ctccatttat atattatata ttacccactt atgtattata
1800 ttaggatgtt aaggagacat aacaattata aagagagaag tttgtatcca
tttatatatt 1860 atatactacc catttatata ttatacttat ccacttattt
aatgtcttta taaggtttga 1920 tccatgatat ttctaatatt ttagttgata
tgtatatgaa agggtactat ttgaactctc 1980 ttactctgta taaaggttgg
atcatcctta aagtgggtct atttaatttt attgcttctt 2040 acagataaaa
aaaaaattat gagttggttt gataaaatat tgaaggattt aaaataataa 2100
taaataacat ataatatatg tatataaatt tattataata taacatttat ctataaaaaa
2160 gtaaatattg tcataaatct atacaatcgt ttagccttgc tggacgaatc
tcaattattt 2220 aaacgagagt aaacatattt gactttttgg ttatttaaca
aattattatt taacactata 2280 tgaaattttt ttttttatca gcaaagaata
aaattaaatt aagaaggaca atggtgtccc 2340 aatccttata caaccaactt
ccacaagaaa gtcaagtcag agacaacaaa aaaacaagca 2400 aaggaaattt
tttaatttga gttgtcttgt ttgctgcata atttatgcag taaaacacta 2460
cacataaccc ttttagcagt agagcaatgg ttgaccgtgt gcttagcttc ttttatttta
2520 tttttttatc agcaaagaat aaataaaata aaatgagaca cttcagggat
gtttcaacaa 2580 gcttggcgcg ccgttctata gtgtcaccta aatcgtatgt
gtatgataca taaggttatg 2640 tattaattgt agccgcgttc taacgacaat
atgtccatat ggtgcactct cagtacaatc 2700 tgctctgatg ccgcatagtt
aagccagccc cgacacccgc caacacccgc tgacgcgccc 2760 tgacgggctt
gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 2820
tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
2880 atacgcctat ttttataggt taatgtcatg accaaaatcc cttaacgtga
gttttcgttc 2940 cactgagcgt cagaccccgt agaaaagatc aaaggatctt
cttgagatcc tttttttctg 3000 cgcgtaatct gctgcttgca aacaaaaaaa
ccaccgctac cagcggtggt ttgtttgccg 3060 gatcaagagc taccaactct
ttttccgaag gtaactggct tcagcagagc gcagatacca 3120 aatactgtcc
ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 3180
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
3240 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
gtcgggctga 3300 acggggggtt cgtgcacaca gcccagcttg gagcgaacga
cctacaccga actgagatac 3360 ctacagcgtg agcattgaga aagcgccacg
cttcccgaag ggagaaaggc ggacaggtat 3420 ccggtaagcg gcagggtcgg
aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3480 tggtatcttt
atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3540
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
3600 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
tgattctgtg 3660 gataaccgta ttaccgcctt tgagtgagct gataccgctc
gccgcagccg aacgaccgag 3720 cgcagcgagt cagtgagcga ggaagcggaa
gagcgcccaa tacgcaaacc gcctctcccc 3780 gcgcgttggc cgattcatta
atgcaggttg atcagatctc gatcccgcga aattaatacg 3840 actcactata
gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga 3900
aggagatata cccatggaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
3960 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
aatctcgtgc 4020 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
gtaaatagct gcgccgatgg 4080 tttctacaaa gatcgttatg tttatcggca
ctttgcatcg gccgcgctcc cgattccgga 4140 agtgcttgac attggggaat
tcagcgagag cctgacctat tgcatctccc gccgtgcaca 4200 gggtgtcacg
ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc 4260
ggaggctatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
4320 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
cgattgctga 4380 tccccatgtg tatcactggc aaactgtgat ggacgacacc
gtcagtgcgt ccgtcgcgca 4440 ggctctcgat gagctgatgc tttgggccga
ggactgcccc gaagtccggc acctcgtgca 4500 cgcggatttc ggctccaaca
atgtcctgac ggacaatggc cgcataacag cggtcattga 4560 ctggagcgag
gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag 4620
gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
4680 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
aactctatca 4740 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
cagggtcgat gcgacgcaat 4800 cgtccgatcc ggagccggga ctgtcgggcg
tacacaaatc gcccgcagaa gcgcggccgt 4860 ctggaccgat ggctgtgtag
aagtactcgc cgatagtgga aaccgacgcc ccagcactcg 4920 tccgagggca
aaggaatagt gaggtacagc ttggatcgat ccggctgcta acaaagcccg 4980
aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
5040 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
gatgatcggg 5100 cgcgccgtcg acggatccgt acgcaaaggc aaagatttaa
actcgaaaac attacaaaag 5160 tctcaaaaca gaggcaaggc catgcacaaa
gcacactcta agtgcttcca ttgcctacta 5220 agtagggtac gtacacgatc
accattcacc agtgatgatc tttattaata tacaacacac 5280 tcagagacag
cttatgttat agctagctag cataaactat cacatcatgt gttagtacga 5340
caagtgacaa cattgctttt aacttcgcgg ccttggatcc tctagaccgg atataatgag
5400 ccgtaaacaa agatgattaa gtagtaatta atacgtacta gtaaaagtgg
caaaagataa 5460 cgagaaagaa ccaatttctt tgcattcggc cttagcggaa
ggcatatata agctttgatt 5520 attttattta gtgtaatgat ttcgtacaac
caaagcattt atttagtact ctcacacttg 5580 tgtcgcggcc gcgaattcac
tagtgattcc ttatagagcc ttccccgcgg gttgcttctc 5640 cgccatccgg
gcgaacaccg ccagatagcg cagcaggatg accaaccctt catggggcag 5700
cgggttccga tacggcaggt tgtgcttctg gcacagctgt tccacctggt agctaaccgc
5760 tgtcaggttg tggcgaggga gggtcggcca caaatggtgc tcaatctggt
aattcaagcc 5820 tccgaaaaac caatctgtga taatccctcg ccgaatgttc
atggtctcat ggatctggcc 5880 aaccgagaat ccatggccat cccagactga
gtccccgatc ttctccagtg ggtagtggtt 5940 catgaacacc acgatcgcaa
tgccgaagcc gccaaccagc tccgaaacga aaaacaccaa 6000 cagcgatgtg
aggatgctgg gcataaagaa taagtggaac agggtcttca aggtccagtg 6060
cagggcgagg ccaatggcct ccttcttata ctgagagcga tagaattggt tatctctgtc
6120 cttcaaactg cgcacggtca acacgctctg gaaacaccaa atgaaccgca
acaagataca 6180 gatgaccaag aaatagtact gctggaactg aatgagcttg
cgggaaatcg gtgacgcccg 6240 tgtgacgtca tcctcagacc aggctaagag
ggggaggttg tcaatatcag ggtcgtgccc 6300 ttgaacattg gttgccgaat
gatgtgcatt gtgtctgtcc ttccaccatg tcacggaaaa 6360 accttgcaga
ccattgccaa ataccagtcc cacgaggttg ttccagttcc ggttcttgaa 6420
agtctggtgg tggcaaatgt catgagaaag ccagcccatc tgttgatagt gcatcccaag
6480 caacactgcc ccaatgaaat acatctgata ctgaaccatc aggaaataac
ccagcactcc 6540 aaggcccagt gtggtgctga ttttgtatga gtaccagagg
ggggaggcat caaacatgcc 6600 agttgcgatc aactcttctc ggagcttccg
gaaatcctct tgagcttcat tcactgcagc 6660 ctggggtggc aactcagaac
tgggattgat tttgggcatg cgcttgagct tgtcgaaggc 6720 ttcttgagag
tgcataacca tgaaggcatc agtggcatcc cttccttggt aattctctat 6780
aatttccgca ccaccagggt ggaaattgac ccaggcagac acatcatatg ttgttccatc
6840 aattgtaagg ggaagcgctt ggcgctttga cttcatttca atcgaattcc
cgcggccgct 6900 tggggggcta tggaagactt tcttagttag ttgtgtgaat
aagcaatgtt gggagaatcg 6960 ggactactta taggatagga ataaaacaga
aaagtattaa gtgctaatga aatatttaga 7020 ctgataatta aaatcttcac
gtatgtccac ttgatataaa aacgtcagga ataaaggaag 7080 tacagtagaa
tttaaaggta ctctttttat atatacccgt gttctctttt tggctagcta 7140
gttgcataaa aaataatcta tatttttatc attattttaa atatcttatg agatggtaaa
7200 tatttatcat aatttttttt actattattt attatttgtg tgtgtaatac
atatagaagt 7260 taattacaaa ttttatttac tttttcatta ttttgatatg
attcaccatt aatttagtgt 7320 tattatttat aatagttcat tttaatcttt
ttgtatatat tatgcgtgca gtactttttt 7380 cctacatata actactatta
cattttattt atataatatt tttattaatg aattttcgtg 7440 ataatatgta
atattgttca ttattatttc agatttttta aaaatatttg tgttattatt 7500
tatgaaatat gtaatttttt tagtatttga ttttatgatg ataaagtgtt ctaaattcaa
7560 aagaaggggg aaagcgtaaa cattaaaaaa cgtcatcaaa caaaaacaaa
atcttgttaa 7620 taaagataaa actgtttgtt ttgatcactg ttatttcgta
atataaaaac attatttata 7680 tttatattgt tgacaaccaa atttgcctat
caaatctaac caatataatg catgcgtggc 7740 aggtaatgta ctaccatgaa
cttaagtcat gacataataa accgtgaatc tgaccaatgc 7800 atgtacctan
ctaaattgta tttgtgacac gaagcaaatg attcaattca caatggagat 7860
gggaaacaaa taatgaagaa cccagaacta agaaagcttt tctgaaaaat aaaataaagg
7920 caatgtcaaa agtatactgc atcatcagtc cagaaagcac atgatatttt
tttatcagta 7980 tcaatgcagc tagttttatt ttacaatatc gatatagcta
gtttaaatat attgcagcta 8040 gatttataaa tatttgtgtt attatttatc
atttgtgtaa tcctgttttt agtattttag 8100 tttatatatg atgataatgt
attccaaatt taaaagaagg gaaataaatt taaacaagaa 8160 aaaaagtcat
caaacaaaaa acaaatgaaa gggtggaaag atgttaccat gtaatgtgaa 8220
tgttacagta tttcttttat tatagagtta acaaattaac taatatgatt ttgttaataa
8280 tgataaaata ttttttttat tattatttca taatataaaa atagtttact
taatataaaa 8340 aaaattctat cgttcacaac aaagttggcc acctaattta
accatgcatg tacccatgga 8400 ccatattagg taaccatcaa acctgatgaa
gagataaaga gatgaagact taagtcataa 8460 cacaaaacca taaaaaacaa
aaatacaatc aaccgtcaat ctgaccaatg catgaaaaag 8520 ctgcaatagt
gagtggcgac acaaagcaca tgattttctt acaacggaga taaaaccaaa 8580
aaaatatttc atgaacaacc tagaacaaat aaagctttta tataataaat atataaataa
8640 ataaaggcta tggaataata tacttcaata tatttggatt aaataaattg
ttggcggggt 8700 tgatatattt atacacacct aaagtcactt caatctcatt
ttcacttaac ttttattttt 8760 tttttctttt tatttatcat aaagagaata
ttgataatat actttttaac atatttttat 8820 gacatttttt attggtgaaa
acttattaaa aatcataaat tttgtaagtt agatttattt 8880 aaagagttcc
tcttcttatt ttaaattttt taataaattt ttaaataact aaaatttgtg 8940
ttaaaaatgt taaaaaatgt gttattaacc cttctcttcg aggacgtacg agatccggcc
9000 ggccagatcc tgcaggtctc aatagattaa gaagttggcg tctcattgat
tgaccatggg 9060 ggatcctcta gacgtacgtg aaggttaaac atggtgaata
tgttaccact agctgggatg 9120 cccattagat caaaactgta aaattctccc
gtttcccttc tattcacatg tgagccccct 9180 cccttttctt tctttctcaa
ttttgattga gttaaagtca ccagcaatgc atcactcacc 9240 ctccaaaaaa
tttcttgtac aacttctcgg actatcccaa agctcctttt cctgagatgg 9300
atggtcctgt ctcttgccct tgatgtcttc cttgttcgat tttggcttcc tctaatgtct
9360 ttcttgctag gaatcaccac ctcactcatc tatgttgtcg tagcttctga
aagtctcata 9420 catatcctta gtgttgcact catcttgtat tgaagtgaaa
aagaatgttg ttctcctatc 9480 caaatctcca ttgaatctct ttctcccaat
gttgtcccat cggttggtcc tcctctccaa 9540 ccaattaatt gtaaggtgtt
taacataaac atggtacaat taagattttt catttcatta 9600 agaaaagatt
gagatttgtg gttctaaagt ttcaattaga gtttgatgat attgaaacaa 9660
ccgtagaaca cattaagtat tactaactta tacatagagc attggaattt caccttttat
9720 ttattctgtt tccgccaaag gtacatgact caagttattt tacacaagta
acaaaggcat 9780 ctaagcctaa gtattcttat tcagactttt cattattact
ttcattgatt tggtgcgaaa 9840 tgcggccgct taggaatcct gtgcgtcctt
cacgcagtgg acgacaccca ccttatgcat 9900 gtacttcagg gtggagatga
tgttgccgac gatggtaggg tagaaaacat atcgcactcc 9960 atggcgttcg
caacactccc ggaccgcctg ctggacgaag gggtagtgcc aagacgacat 10020
ccggggaaag aggtggtgct cgatctggaa attgagaccg ccagtgaaga acatggcgat
10080 ggggccaccg tatgtggagg acgtctccac ctgcgcccga taccagtcaa
tttggtcagc 10140 ctcaacgtcc ttctcagatc tcttaacctt gcagttcttg
tcggggatcc gttcggagcc 10200 atcaaaattg tgggacaaaa tgaagaagaa
cgtcaagaag gcaccagttg cggtgggcac 10260 cagaggaatg gtgatcagca
atgaggtccc agtgaggtac cagggcaaga atgcggtccg 10320 gaagatgaag
aaagctcgca tcacgaatgc aagtgtgcgg tagcgccgca gaaagccatt 10380
ttcccgcatg gccgtgacag actctgggat ggtgtcattg tgctgcatcc ggaaaatgta
10440 gagcgggttg tacaccagcg ataccccgta tgcccccagc acaaggtaca
tgtaccaagc 10500 ttggaagcgg tggaaccact ttcgggcggt gtttgcggcg
gggtagttgt ggaacaccag 10560 gaacggctct gccgactccg catccaggtc
gaggccgtgc tggttggtgt aggtgtggtg 10620 ccgcatgatg tgcgactgca
gccaaatcca ccgggacgat ccgatgacgt caatgccgta 10680 ggcgaagagg
gcgttgaccc aaggcttctt gctgatggcc ccgtgggacg catcatgctg 10740
gatggataag ccgatctgca tgtgcatgaa tccagtcaac aggccccaca gcaccatccc
10800 cgtggtagcc cagtgccact cgcaaaaggc cgtcacggcg atgatcccaa
cggtgcgcag 10860 ccagaagcca ggggttgcgt accagttcct ccctttcatc
acctcgcgca ttgtccgctt 10920 aacgtcttgt gcgaagggag aatcatacgt
gtagacaatg tccttcgagg tgcggtcatg 10980 cttccctcgg acgaactgtt
gaagcaattt ggaattctcc ggtttgacgt atggatgcat 11040 tgaataaaag
agaggggagg catcagaggc accagaagcg agaatcaaat ctcctcctgg 11100
atgaactttg gcaagccctt caaggtcgta gaggatgcca tcaatcgcaa ccaaatcagg
11160 gcgttctaac agctgttctg tggtaagact gagagccatg gtttgcggcc
gcggtgatga 11220 ctgatgagtg tttaaggacc aatggagaga atgtttgagt
tgtgaagcgg agaacctgag 11280 gcgtggttat ttatagggaa gagaggaagg
tgaatgaggg acacgtcaca gaagtagggt 11340 gctgagcttg agacattctt
cagtatgcat ggctatggaa gccttgggtg ctacacctca 11400 tgaagttcat
ggtgtgaggt ggcttcggca tctcaattaa gtgacaaaga gaaaggtgtt 11460
tcagtgtttc tattgcaaat ggcagaaact cgtgatgacg aggggaccat gcatggtttc
11520 atttcttttc ttcctggatt ctttctttcc ttttatatat gcaggttcat
aatttaaaaa 11580 ttagactcgc tttcaatttc ttaatttctc attttcctct
tatattactg tactaatgtt 11640 aaccacgtac acttattttt tttttagttt
aattttgata gattgtgttg atttaaacat 11700 attaatattt tcaaccaaat
aaaaatcatt ttagtagata cggcttttta aataattatt 11760 aaaaatatta
actatttatc ctaaatggca cattttaatt aaaaaaaatc cggtgttgta 11820
agtgttttat taatttgttt tggcattatt aaagcaactt tttttttatt tgttggcatt
11880 ttgagtacgt acttaggcta gcctgca 11907 <210> SEQ ID NO 93
<211> LENGTH: 8671 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR328 <400> SEQUENCE: 93
ggatctggcc ggccggatct cgtacggatc cgtcgacggc gcgcccgatc atccggatat
60 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
ggggttatgc 120 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
tttcgggctt tgttagcagc 180 cggatcgatc caagctgtac ctcactattc
ctttgccctc ggacgagtgc tggggcgtcg 240 gtttccacta tcggcgagta
cttctacaca gccatcggtc cagacggccg cgcttctgcg 300 ggcgatttgt
gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc 360
ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
420 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
gatgcctccg 480 ctcgaagtag cgcgtctgct gctccataca agccaaccac
ggcctccaga agaagatgtt 540 ggcgacctcg tattgggaat ccccgaacat
cgcctcgctc cagtcaatga ccgctgttat 600 gcggccattg tccgtcagga
cattgttgga gccgaaatcc gcgtgcacga ggtgccggac 660 ttcggggcag
tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact 720
gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
780 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
cgaacccgct 840 cgtctggcta agatcggccg cagcgatcgc atccatagcc
tccgcgaccg gctgcagaac 900 agcgggcagt tcggtttcag gcaggtcttg
caacgtgaca ccctgtgcac ggcgggagat 960 gcaataggtc aggctctcgc
tgaattcccc aatgtcaagc acttccggaa tcgggagcgc 1020 ggccgatgca
aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt 1080
tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
1140 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
acttctcgac 1200 agacgtcgcg gtgagttcag gcttttccat gggtatatct
ccttcttaaa gttaaacaaa 1260 attatttcta gagggaaacc gttgtggtct
ccctatagtg agtcgtatta atttcgcggg 1320 atcgagatcg atccaattcc
aatcccacaa aaatctgagc ttaacagcac agttgctcct 1380 ctcagagcag
aatcgggtat tcaacaccct catatcaact actacgttgt gtataacggt 1440
ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa cggcgttccc
1500 ggagttgcac acaagaaatt tgccactatt acagaggcaa gagcagcagc
tgacgcgtac 1560 acaacaagtc agcaaacaga caggttgaac ttcatcccca
aaggagaagc tcaactcaag 1620 cccaagagct ttgctaaggc cctaacaagc
ccaccaaagc aaaaagccca ctggctcacg 1680 ctaggaacca aaaggcccag
cagtgatcca gccccaaaag agatctcctt tgccccggag 1740 attacaatgg
acgatttcct ctatctttac gatctaggaa ggaagttcga aggtgaaggt 1800
gacgacacta tgttcaccac tgataatgag aaggttagcc tcttcaattt cagaaagaat
1860 gctgacccac agatggttag agaggcctac gcagcaggtc tcatcaagac
gatctacccg 1920 agtaacaatc tccaggagat caaatacctt cccaagaagg
ttaaagatgc agtcaaaaga 1980 ttcaggacta attgcatcaa gaacacagag
aaagacatat ttctcaagat cagaagtact 2040 attccagtat ggacgattca
aggcttgctt cataaaccaa ggcaagtaat agagattgga 2100 gtctctaaaa
aggtagttcc tactgaatct aaggccatgc atggagtcta agattcaaat 2160
cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
2220 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
tggtctactc 2280 caaaaatgtc aaagatacag tctcagaaga ccaaagggct
attgagactt ttcaacaaag 2340 gataatttcg ggaaacctcc tcggattcca
ttgcccagct atctgtcact tcatcgaaag 2400 gacagtagaa aaggaaggtg
gctcctacaa atgccatcat tgcgataaag gaaaggctat 2460 cattcaagat
gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 2520
cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgacatctc
2580 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
cctctatata 2640 aggaagttca tttcatttgg agaggacacg ctcgagctca
tttctctatt acttcagcca 2700 taacaaaaga actcttttct cttcttatta
aaccatgaaa aagcctgaac tcaccgcgac 2760 gtctgtcgag aagtttctga
tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 2820 ggagggcgaa
gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 2880
ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
2940 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
gcctgaccta 3000 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
ctgcctgaaa ccgaactgcc 3060 cgctgttctg cagccggtcg cggaggccat
ggatgcgatc gctgcggccg atcttagcca 3120 gacgagcggg ttcggcccat
tcggaccgca aggaatcggt caatacacta catggcgtga 3180 tttcatatgc
gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 3240
cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
3300 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
cggacaatgg 3360 ccgcataaca gcggtcattg actggagcga ggcgatgttc
ggggattccc aatacgaggt 3420 cgccaacatc ttcttctgga ggccgtggtt
ggcttgtatg gagcagcaga cgcgctactt 3480 cgagcggagg catccggagc
ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 3540 tggtcttgac
caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 3600
gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
3660 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
ccgatagtgg 3720 aaaccgacgc cccagcactc gtccgagggc aaaggaatag
tgaggtacct aaagaaggag 3780 tgcgtcgaag cagatcgttc aaacatttgg
caataaagtt tcttaagatt gaatcctgtt 3840 gccggtcttg cgatgattat
catataattt ctgttgaatt acgttaagca tgtaataatt 3900 aacatgtaat
gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta 3960
tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
4020 gcggtgtcat ctatgttact agatcgatgt cgaatcgatc aacctgcatt
aatgaatcgg 4080 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
tccgcttcct cgctcactga 4140 ctcgctgcgc tcggtcgttc ggctgcggcg
agcggtatca gctcactcaa aggcggtaat 4200 acggttatcc acagaatcag
gggataacgc aggaaagaac atgtgagcaa aaggccagca 4260 aaaggccagg
aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4320
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
4380 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
cgaccctgcc 4440 gcttaccgga tacctgtccg cctttctccc ttcgggaagc
gtggcgcttt ctcaatgctc 4500 acgctgtagg tatctcagtt cggtgtaggt
cgttcgctcc aagctgggct gtgtgcacga 4560 accccccgtt cagcccgacc
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4620 ggtaagacac
gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4680
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4740 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
gagttggtag 4800 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
ttttttgttt gcaagcagca 4860 gattacgcgc agaaaaaaag gatctcaaga
agatcctttg atcttttcta cggggtctga 4920 cgctcagtgg aacgaaaact
cacgttaagg gattttggtc atgacattaa cctataaaaa 4980 taggcgtatc
acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 5040
acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
5100 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
actatgcggc 5160 atcagagcag attgtactga gagtgcacca tatggacata
ttgtcgttag aacgcggcta 5220 caattaatac ataaccttat gtatcataca
catacgattt aggtgacact atagaacggc 5280 gcgccaagct tggatctcct
gcagcccggg ggatccgccc acgtacggta ccatctgcta 5340 atattttaaa
tcacatgcaa gagaggaggc atggttccat tttctacctt cacattattt 5400
gagaaaaacg aacttgttct gtgttttatt tttgcccttc acattagtac aacgtggaag
5460 actcatggtt acacagaatc atacataagt acaatgcttg tccctaagaa
aacaagcact 5520 cgttgtattg aacctttacg gctcatgcgg ccgcgaattc
actagtgatt gaattcgcgg 5580 ccgcttagtc cgacttggcc ttggcggccg
cggccgactc tttgagcgtg aagatctgcg 5640 ccgtctcggg cacagcgccg
tagttgacaa agaggtgcgc ggtcttgaag aaggccgtga 5700 tgatgggctc
gtcgttcctg cgcacgaggt gcgggtacgc ggccgcaaag tgcttggtgg 5760
cttcgttgag cttgtagtgc ggaatgatcg ggaacaagtg gtggacctgg tgcgtgccaa
5820 tgtggtggct caggttgtcc acgaacgcgc cgtacgagcg gtcgacgctc
gagaggttgc 5880 ccttgacgta cgtccactcc gagtcgccgt accacggcgt
cgcttcgtcg ttgtggtgca 5940 agaaggtcgt aatgacgagg aacgaagcaa
agacaaagag cggcgcatag tagtagaggc 6000 ccatgacggc aaagccgagc
gagtatgtga ggtacgcgta cgcggcgaag aaggcggccc 6060 agacgccgag
cgacacgatg acggccgacg cgcggcgaag gaggagcggg tcccacgggt 6120
caaagtggct catcgtgcgc ggggcatacc cgaccttcaa gtagacaaac cacgcaccgc
6180 cgagcgtgta gacccattgg cgcacgtcct ggaggtcctt gaccgaccgg
tgcgggtaaa 6240 agatctcgtc cttatcaatg ttgcccgtgt tcttgtggtg
gtggcggtgc gtcacgcgcc 6300 agctctcgaa cggcgtcaaa atcgcagagt
gcatgatgca gccgatgata aagttgacgc 6360 tgtggtagcg cgagaaggcc
gagtggccgc agtcgtggcc gaccgtgaag aagccccaga 6420 agatgacgcc
ctgcacgtag atgtaggtgg cgcaaacgag cgcgtggagc agaacgttat 6480
cggcaatgaa cggcgtcgag cgcgccgcgt agagcagcgc cgccgaggcc gacgcgttga
6540 agatcgcgcg ggccgtgtag tagagcgaga ggccgaggtt cgactcaaag
cacgcgttcg 6600 ggatcgagtg cttgagctcc gtgagcgtcg ggaactcgac
cttcgtctta tcctcagtca 6660 tgcggccgct gaagtattgc ttcttagtta
acctttcctt tctctctcag ctatgtgaat 6720 tcattttgct ttcgtcacaa
tttatatagt gaaattggat ctttggagtt aacgccttca 6780 caggattatc
gtgttagaac aatgcttttt catgttctaa ttagtagtac attacaaatg 6840
tgcactctat tcaataagca tcttttggca cgttaataaa tcatgtgaaa aaaaaatact
6900 actatttcaa agaaagtgtt gtaaaaagaa acggaaagag agctggcttc
agttgttgag 6960 acttgtttgc tagtaaaaat ggtgtgaaga gtgattcatg
gtgaggtggt ttttcgtccc 7020 tttctgtttg catgaaaaac aaatggcaag
agatgacgta ggattccttc ccttaacgat 7080 tatctgtttt taatttcaaa
tatacatata ggaatttatg aattactaag gttgtaaaat 7140 atgctggtca
tttatttatg gctaaaatat ttttttttct cgtaaatata aaaatattta 7200
aaatttattt ttatcatatt ttttatcctt ataaaattat gtgtacaacc tatataaaaa
7260 aatatcatat ttaatattga ttatatgttt aatcaatata aaaaatcatt
atcatatatt 7320 tagatttatt cgaatataca tctaaacaaa aaataacata
ttttaatttt atgaagaaaa 7380 aaaaatattt tatcctttat ttatttaaga
ttaattaata gttatgtatt gtggaaagac 7440 ttttacacat gcaatagata
tactgaatca attagatgcc aatgctgagt tggaaatcac 7500 ttgaggaggg
gaggagactt gccaatgctt ttcagtttca tttaaatgat ttagtggagg 7560
agatagagta gtgataaagg catgccccaa ttttggagtg tatatatgag tggaaataag
7620 agagggatag agagaaaaaa taaagagagt aaaaataatt aatgtgaaat
gatatgataa 7680 aaaaataaag aaagagataa agagaaaaat gaaatgagag
atagatgaaa tagagagtag 7740 atacatgttt gtttaggttt tttttaggaa
ataacacatt tttttctcat cacttattac 7800 tcactgtcaa tttcctctct
ttcaatcata atgatatgat ttgtttaaca aaaatgtgaa 7860 aaaacatata
aagtaaaata tttttataaa ttgataaata aaaatttaca aaatttattt 7920
cttattaaat tgaatagaaa atgaaagaaa agaaaagaaa aagtatatat aaaatgatat
7980 agctttaaaa agaataaatt tttcatatca gtcttttttt aataatttag
aaatatttaa 8040 gtatatagca aaaatataat gtactttaca tatgcataaa
taataatttg aaaatagaac 8100 taatagaata gagaaaaaag taatataata
attaactata tgaaaattta gaagggacaa 8160 tatttttaat taagaatata
aacaatattt cttttcatgt aatgagggac ggatgtacgg 8220 ggccagtgtt
ggagtcaaag ccaaaatagt cacggggaaa ttaatgcact gcatgactat 8280
tcgaaaaaat tcactagcct tacttagatg ttagattaat agctaggggg tgcagataat
8340 tttgaaaggc atgaaaaaca ttaatttgta cattgcaagc ttttgatgac
aagctttgca 8400 attgttcaca ctaccttatg ccatttataa atagagtgat
tggcatatga aggaaatcat 8460 gagagtcgaa gcgaaaaaca aagcttgaga
gtgtaggaaa aatacagttt ttttggtaaa 8520 aatacagtat ttgaatagga
gcgaaaaata tcctttcaaa atgatccttt tctttttttt 8580 tttttttctt
gttgttcttg gtcagttatt caaaggaaaa gggattgaaa taaaaacttg 8640
catgtgggat cgtacgtcga gtcgacctgc a 8671 <210> SEQ ID NO 94
<211> LENGTH: 1413 <212> TYPE: DNA <213>
ORGANISM: Saprolegnia diclina <400> SEQUENCE: 94 atggccccgc
agacggagct ccgccagcgc cacgccgccg tcgccgagac gccggtggcc 60
ggcaagaagg cctttacatg gcaggaggtc gcgcagcaca acacggcggc ctcggcctgg
120 atcattatcc gcggcaaggt ctacgacgtg accgagtggg ccaacaagca
ccccggcggc 180 cgcgagatgg tgctgctgca cgccggtcgc gaggccaccg
acacgttcga ctcgtaccac 240 ccgttcagcg acaaggccga gtcgatcttg
aacaagtatg agattggcac gttcacgggc 300 ccgtccgagt ttccgacctt
caagccggac acgggcttct acaaggagtg ccgcaagcgc 360 gttggcgagt
acttcaagaa gaacaacctc catccgcagg acggcttccc gggcctctgg 420
cgcatgatgg tcgtgtttgc ggtcgccggc ctcgccttgt acggcatgca cttttcgact
480 atctttgcgc tgcagctcgc ggccgcggcg ctctttggcg tctgccaggc
gctgccgctg 540 ctccacgtca tgcacgactc gtcgcacgcg tcgtacacca
acatgccgtt cttccattac 600 gtcgtcggcc gctttgccat ggactggttt
gccggcggct cgatggtgtc atggctcaac 660 cagcacgtcg tgggccacca
catctacacg aacgtcgcgg gctcggaccc ggatcttccg 720 gtcaacatgg
acggcgacat ccgccgcatc gtgaaccgcc aggtgttcca gcccatgtac 780
gcattccagc acatctacct tccgccgctc tatggcgtgc ttggcctcaa gttccgcatc
840 caggacttca ccgacacgtt cggctcgcac acgaacggcc cgatccgcgt
caacccgcac 900 gcgctctcga cgtggatggc catgatcagc tccaagtcgt
tctgggcctt ctaccgcgtg 960 taccttccgc ttgccgtgct ccagatgccc
atcaagacgt accttgcgat cttcttcctc 1020 gccgagtttg tcacgggctg
gtacctcgcg ttcaacttcc aagtaagcca tgtctcgacc 1080 gagtgcggct
acccatgcgg cgacgaggcc aagatggcgc tccaggacga gtgggcagtc 1140
tcgcaggtca agacgtcggt cgactacgcc catggctcgt ggatgacgac gttccttgcc
1200 ggcgcgctca actaccaggt cgtgcaccac ttgttcccca gcgtgtcgca
gtaccactac 1260 ccggcgatcg cgcccatcat cgtcgacgtc tgcaaggagt
acaacatcaa gtacgccatc 1320 ttgccggact ttacggcggc gttcgttgcc
cacttgaagc acctccgcaa catgggccag 1380 cagggcatcg ccgccacgat
ccacatgggc taa 1413
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 94 <210>
SEQ ID NO 1 <211> LENGTH: 1350 <212> TYPE: DNA
<213> ORGANISM: Euglena gracilis <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(1350) <223> OTHER INFORMATION: delta-5 desaturase
<400> SEQUENCE: 1 atggctctca gtcttaccac agaacagctg ttagaacgcc
ctgatttggt tgcgattgat 60 ggcatcctct acgaccttga agggcttgcc
aaagttcatc caggaggaga tttgattctc 120 gcttctggtg cctctgatgc
ctcccctctc ttttattcaa tgcatccata cgtcaaaccg 180 gagaattcca
aattgcttca acagttcgtc cgagggaagc atgaccgcac ctcgaaggac 240
attgtctaca cgtatgattc tcccttcgca caagacgtta agcggacaat gcgcgaggtg
300 atgaaaggga ggaactggta cgcaacccct ggcttctggc tgcgcaccgt
tgggatcatc 360 gccgtgacgg ccttttgcga gtggcactgg gctaccacgg
ggatggtgct gtggggcctg 420 ttgactggat tcatgcacat gcagatcggc
ttatccatcc agcatgatgc gtcccacggg 480 gccatcagca agaagccttg
ggtcaacgcc ctcttcgcct acggcattga cgtcatcgga 540 tcgtcccggt
ggatttggct gcagtcgcac atcatgcggc accacaccta caccaaccag 600
cacggcctcg acctggatgc ggagtcggca gagccgttcc tggtgttcca caactacccc
660 gccgcaaaca ccgcccgaaa gtggttccac cgcttccaag cttggtacat
gtaccttgtg 720 ctgggggcat acggggtatc gctggtgtac aacccgctct
acattttccg gatgcagcac 780 aatgacacca tcccagagtc tgtcacggcc
atgcgggaga atggctttct gcggcgctac 840 cgcacacttg cattcgtgat
gcgagctttc ttcatcttcc ggaccgcatt cttgccctgg 900 tacctcactg
ggacctcatt gctgatcacc attcctctgg tgcccactgc aactggtgcc 960
ttcttgacgt tcttcttcat tttgtcccac aattttgatg gctccgaacg gatccccgac
1020 aagaactgca aggttaagag ctctgagaag gacgttgagg ctgaccaaat
tgactggtat 1080 cgggcgcagg tggagacgtc ctccacatac ggtggcccca
tcgccatgtt cttcactggc 1140 ggtctcaatt tccagatcga gcaccacctc
tttccccgga tgtcgtcttg gcactacccc 1200 ttcgtccagc aggcggtccg
ggagtgttgc gaacgccatg gagtgcgata tgttttctac 1260 cctaccatcg
tcggcaacat catctccacc ctgaagtaca tgcataaggt gggtgtcgtc 1320
cactgcgtga aggacgcaca ggattcctga 1350 <210> SEQ ID NO 2
<211> LENGTH: 449 <212> TYPE: PRT <213> ORGANISM:
Euglena gracilis <400> SEQUENCE: 2 Met Ala Leu Ser Leu Thr
Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 1 5 10 15 Val Ala Ile Asp
Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 20 25 30 His Pro
Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 35 40 45
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 50
55 60 Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys
Asp 65 70 75 80 Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val
Lys Arg Thr 85 90 95 Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr
Ala Thr Pro Gly Phe 100 105 110 Trp Leu Arg Thr Val Gly Ile Ile Ala
Val Thr Ala Phe Cys Glu Trp 115 120 125 His Trp Ala Thr Thr Gly Met
Val Leu Trp Gly Leu Leu Thr Gly Phe 130 135 140 Met His Met Gln Ile
Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 145 150 155 160 Ala Ile
Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 165 170 175
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 180
185 190 Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala
Glu 195 200 205 Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala
Ala Asn Thr 210 215 220 Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp
Tyr Met Tyr Leu Val 225 230 235 240 Leu Gly Ala Tyr Gly Val Ser Leu
Val Tyr Asn Pro Leu Tyr Ile Phe 245 250 255 Arg Met Gln His Asn Asp
Thr Ile Pro Glu Ser Val Thr Ala Met Arg 260 265 270 Glu Asn Gly Phe
Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 275 280 285 Ala Phe
Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 290 295 300
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 305
310 315 320 Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly
Ser Glu 325 330 335 Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser
Glu Lys Asp Val 340 345 350 Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala
Gln Val Glu Thr Ser Ser 355 360 365 Thr Tyr Gly Gly Pro Ile Ala Met
Phe Phe Thr Gly Gly Leu Asn Phe 370 375 380 Gln Ile Glu His His Leu
Phe Pro Arg Met Ser Ser Trp His Tyr Pro 385 390 395 400 Phe Val Gln
Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 405 410 415 Tyr
Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 420 425
430 Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp
435 440 445 Ser <210> SEQ ID NO 3 <211> LENGTH: 1350
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <223>
OTHER INFORMATION: synthetic delta-5 desaturase (codon-optimized)
for Yarrowia lipolytica <400> SEQUENCE: 3 atggctctct
cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac 60
ggcattctct acgatctgga aggtcttgcc aaggtccatc ccggaggcga cttgatcctc
120 gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta
cgtcaagccc 180 gagaactcga agctgcttca acagttcgtg cgaggcaagc
acgaccgaac ctccaaggac 240 attgtctaca cctacgactc tccctttgca
caggacgtca agcgaactat gcgagaggtc 300 atgaaaggtc ggaactggta
tgccacacct ggattctggc tgcgaaccgt tggcatcatt 360 gctgtcaccg
ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc 420
ttgactggat tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt
480 gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga
cgtcattgga 540 tcgtccagat ggatctggct gcagtctcac atcatgcgac
atcacaccta caccaatcag 600 catggtctcg acctggatgc cgagtccgca
gaaccattcc ttgtgttcca caactaccct 660 gctgccaaca ctgctcgaaa
gtggtttcac cgattccagg cctggtacat gtacctcgtg 720 cttggagcct
acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac 780
aacgacacca ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac
840 cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt
cttgccctgg 900 tatctcactg gaacctccct gctcatcacc attcctctgg
tgcccactgc taccggtgcc 960 ttcctcacct tctttttcat cttgtctcac
aacttcgatg gctcggagcg aatccccgac 1020 aagaactgca aggtcaagag
ctccgagaag gacgttgaag ccgatcagat cgactggtac 1080 agagctcagg
tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc 1140
ggtctcaact tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc
1200 ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta
cgtcttctac 1260 cctaccattg tgggcaacat catttccacc ctcaagtaca
tgcacaaagt cggtgtggtt 1320 cactgtgtca aggacgctca ggattcctaa 1350
<210> SEQ ID NO 4 <211> LENGTH: 590 <212> TYPE:
DNA <213> ORGANISM: Euglena gracilis <400> SEQUENCE: 4
ggccaccaca tctacaccaa ccagcacggc ctcgacctgg atgcggagtc ggcagagccg
60 ttcctggtgt tccacaacta ccccgccgca aacaccgccc gaaagtggtt
ccaccgcttc 120 caagcttggt acatgtacct tgtgctgggg gcatacgggg
tatcgctggt gtacaacccg 180 ctctacattt tccggatgca gcacaatgac
accatcccag agtctgtcac ggccatgcgg 240 gaaaatggct ttctgcggcg
ctaccgcaca cttgcattcg tgatgcgagc tttcttcatc 300 ttccggaccg
cattcttgcc ctggtacctc actgggacct cattgctgat caccattcct 360
ctggtgccca ccgcaactgg tgccttcttg acgttcttct tcattttgtc ccacaatttt
420 gatggctccg aacggatccc cgacaagaac tgcaaggtta agagctctga
gaaggacgtt 480 gaggctgacc aaattgactg gtatcgggcg caggtggaga
cgtcctccac atacggtggc 540 cccatcgcca tgttcttcac tggcggtctc
aactaccaaa tcgtccacca 590 <210> SEQ ID NO 5 <211>
LENGTH: 196 <212> TYPE: PRT <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 5
Gly His His Ile Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 1 5
10 15 Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn
Thr 20 25 30 Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met
Tyr Leu Val 35 40 45 Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn
Pro Leu Tyr Ile Phe 50 55 60 Arg Met Gln His Asn Asp Thr Ile Pro
Glu Ser Val Thr Ala Met Arg 65 70 75 80 Glu Asn Gly Phe Leu Arg Arg
Tyr Arg Thr Leu Ala Phe Val Met Arg 85 90 95 Ala Phe Phe Ile Phe
Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 100 105 110 Thr Ser Leu
Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 115 120 125 Phe
Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 130 135
140 Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val
145 150 155 160 Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu
Thr Ser Ser 165 170 175 Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr
Gly Gly Leu Asn Tyr 180 185 190 Gln Ile Val His 195 <210> SEQ
ID NO 6 <211> LENGTH: 797 <212> TYPE: DNA <213>
ORGANISM: Euglena gracilis <400> SEQUENCE: 6 cacagaacag
ctgttagaac gccctgattt ggttgcgatt gatggcatcc tctacgacct 60
tgaagggctt gccaaagttc atccaggagg agatttgatt ctcgcttctg gtgcctctga
120 tgcctcccct ctcttttatt caatgcatcc atacgtcaaa ccggagaatt
ccaaattgct 180 tcaacagttc gtccgaggga agcatgaccg cacctcgaag
gacattgtct acacgtatga 240 ttctcccttc gcacaagacg ttaagcggac
aatgcgcgag gtgatgaaag ggaggaactg 300 gtacgcaacc cctggcttct
ggctgcgcac cgttgggatc atcgccgtga cggccttttg 360 cgagtggcac
tgggctacca cggggatggt gctgtggggc ctgttgactg gattcatgca 420
catgcagatc ggcttatcca tccagcatga tgcgtcccac ggggccatca gcaaggagcc
480 ttgggtcaac gccctcttcg cctacggcat tgacgtcatc ggatcgtccc
ggtggatttg 540 gctgcagtca cacatcatgc ggcaccacac ctacaccaac
cagcacggcc tcgacctgga 600 tgcggagtcg gcagagccgt tcctggtgtt
ccacagctac cccgccgcaa acaccgcccg 660 aaagtggttc caccgcttcc
aagcttggta catgtacctt gcgctggggg catacggggt 720 atcgctggtg
tacaacccgc tctacatttt ccggatgcag cacaatgaca ccatcccaga 780
gtctgtcacg gccatgc 797 <210> SEQ ID NO 7 <211> LENGTH:
559 <212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<400> SEQUENCE: 7 cacagaacag ctgttagaac gccctgattt ggttgcgatt
gatggcatcc tctacgacct 60 tgaagggctt gccaaagttc atccaggagg
agatttgatt ctcgcttctg gtgcctctga 120 tgcctcccct ctcttttatt
caatgcatcc atacgtcaaa ccggagaatt ccaaattgct 180 tcaacagttc
gtccgaggga agcatgaccg cacctcgaag gacattgtct acacgtatga 240
ttctcccttc gcacaagacg ttaagcggac aatgcgcgag gtgatgaaag ggaggaactg
300 gtacgcaacc cctggcttct ggctgcgcac cgttgggatc atcgccgtga
cggccttttg 360 cgagtggcac tgggctacca cggggatggt gctgtggggc
ctgttgactg gattcatgca 420 catgcagatc ggcttatcca tccagcatga
tgcgtcccac ggggccatca gcaaggagcc 480 ttgggtcaac gccctcttcg
cctacggcat tgacgtcatc ggatcgtccc ggtggatttg 540 gctgcagtca
cacatcatg 559 <210> SEQ ID NO 8 <211> LENGTH: 273
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<400> SEQUENCE: 8 ggaatggctc tcagtcttac cacagaacag ctgttagaac
gccctgattt ggttgcgatt 60 gatggcatcc tctacgacct tgaagggctt
gccaaagttc atccaggagg agatttgatt 120 ctcgcttctg gtgcctctga
tgcctcccct ctcttttatt caatgcatcc atacgtcaaa 180 ccggagaatt
ccaaattgct tcaacagttc gtccgaggga agcatgaccg cacctcgaag 240
gacattgtct acacgtatga ttctcccttc gca 273 <210> SEQ ID NO 9
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Euglena gracilis <400> SEQUENCE: 9 ggaatggctc tcagtcttac 20
<210> SEQ ID NO 10 <211> LENGTH: 728 <212> TYPE:
DNA <213> ORGANISM: Euglena gracilis <400> SEQUENCE: 10
cctcactggg acctcattgc tgatcaccat tcctctggtg cccacygcaa ctggtgcctt
60 cttgacgttc ttcttcattt tgtcccacaa ttttgatggc tccgaacgga
tccccgacaa 120 gaactgcaag gttaagagct ctgagaagga cgttgaggct
gaccaaattg actggtatcg 180 ggcgcaggtg gagacgtcct ccacatacgg
tggccccatc gccatgttct tcactggcgg 240 tctcaatttc cagatcgagc
accacctctt tccccggatg tcgtcttggc actacccctt 300 cgtccagcag
gcggtccggg agtgttgcga acgccatgga gtgcgatatg ttttctaccc 360
taccatcgtc ggcaacatca tctccaccct gaagtacatg cataaggtgg gtgtcgtcca
420 ctgcgtgaag gacgcacagg attcctgagg ggcagggtga ccaagaacga
tcatcgatgt 480 gttcttctgg ccctttggtg gggtactcgc cagattgctc
cactgaaccg tatcctaact 540 ctgaccctct ccaaccctgt gttgagtgtt
tgctttatgc tccaaagtgg cttcttattt 600 ggtgacccgt ggggcagcgg
cacctgtgcc atgcagctga taacccaggc tgctactcta 660 agttggatgg
ccaatcgtct ggcattgata tccctgctca gcgttgcatt ccaatggttt 720 gcttatcc
728 <210> SEQ ID NO 11 <211> LENGTH: 464 <212>
TYPE: DNA <213> ORGANISM: Euglena gracilis <400>
SEQUENCE: 11 cctctttccc cggatgtcgt cttggcacta ccccttcgtc cagcaggcgg
tccgggagtg 60 ttgcgaacgc catggagtgc gatatgtttt ctaccctacc
atcgtcggca acatcatctc 120 caccctgaag tacatgcata aggtgggtgt
cgtccactgc gtgaaggacg cacaggattc 180 ctgaggggca gggtgaccaa
gaacgatcat cgatgtgttc ttctggccct ttggtggggt 240 actcgccaga
ttgctccact gaaccgtatc ctaactctga ccctctccaa ccctgtgttg 300
agtgtttgct ttatgctcca aagtggcttc ttatttggtg acccgtgggg cagcggcacc
360 tgtgccatgc agctgataac ccaggctgct actctaagtt ggatggccaa
tcgtctggca 420 ttgatatccc tgctcagcgt tgcattccaa tggtttgctt atcc 464
<210> SEQ ID NO 12 <211> LENGTH: 456 <212> TYPE:
PRT <213> ORGANISM: Pythium irregulare <220> FEATURE:
GenBank Accession No. AAL13311 <221> NAME/KEY: MISC_FEATURE
<223> OTHER INFORMATION: delta-5 desaturase <400>
SEQUENCE: 12 Met Gly Thr Asp Gln Gly Lys Thr Phe Thr Trp Gln Glu
Val Ala Lys 1 5 10 15 His Asn Thr Ala Lys Ser Ala Trp Val Ile Ile
Arg Gly Glu Val Tyr 20 25 30 Asp Val Thr Glu Trp Ala Asp Lys His
Pro Gly Gly Ser Glu Leu Ile 35 40 45 Val Leu His Ser Gly Arg Glu
Cys Thr Asp Thr Phe Tyr Ser Tyr His 50 55 60 Pro Phe Ser Asn Arg
Ala Asp Lys Ile Leu Ala Lys Tyr Lys Ile Gly 65 70 75 80 Lys Leu Val
Gly Gly Tyr Glu Phe Pro Val Phe Lys Pro Asp Ser Gly 85 90 95 Phe
Tyr Lys Glu Cys Ser Glu Arg Val Ala Glu Tyr Phe Lys Thr Asn 100 105
110 Asn Leu Asp Pro Lys Ala Ala Phe Ala Gly Leu Trp Arg Met Val Phe
115 120 125 Val Phe Ala Val Ala Ala Leu Ala Tyr Met Gly Met Asn Glu
Leu Ile 130 135 140 Pro Gly Asn Val Tyr Ala Gln Tyr Ala Trp Gly Val
Val Phe Gly Val 145 150 155 160 Phe Gln Ala Leu Pro Leu Leu His Val
Met His Asp Ser Ser His Ala 165 170 175 Ala Cys Ser Ser Ser Pro Ala
Met Trp Gln Ile Ile Gly Arg Gly Val 180 185 190 Met Asp Trp Phe Ala
Gly Ala Ser Met Val Ser Trp Leu Asn Gln His 195 200 205 Val Val Gly
His His Ile Tyr Thr Asn Val Ala Gly Ala Asp Pro Asp 210 215 220 Leu
Pro Val Asp Phe Glu Ser Asp Val Arg Arg Ile Val His Arg Gln 225 230
235 240 Val Leu Leu Pro Ile Tyr Lys Phe Gln His Ile Tyr Leu Pro Pro
Leu 245 250 255 Tyr Gly Val Leu Gly Leu Lys Phe Arg Ile Gln Asp Val
Phe Glu Thr 260 265 270
Phe Val Ser Leu Thr Asn Gly Pro Val Arg Val Asn Pro His Pro Val 275
280 285 Ser Asp Trp Val Gln Met Ile Phe Ala Lys Ala Phe Trp Thr Phe
Tyr 290 295 300 Arg Ile Tyr Ile Pro Leu Val Trp Leu Lys Ile Thr Pro
Ser Thr Phe 305 310 315 320 Trp Gly Val Phe Phe Leu Ala Glu Phe Thr
Thr Gly Trp Tyr Leu Ala 325 330 335 Phe Asn Phe Gln Val Ser His Val
Ser Thr Glu Cys Glu Tyr Pro Cys 340 345 350 Gly Asp Ala Pro Ser Ala
Glu Val Gly Asp Glu Trp Ala Ile Ser Gln 355 360 365 Val Lys Ser Ser
Val Asp Tyr Ala His Gly Ser Pro Leu Ala Ala Phe 370 375 380 Leu Cys
Gly Ala Leu Asn Tyr Gln Val Thr His His Leu Tyr Pro Gly 385 390 395
400 Ile Ser Gln Tyr His Tyr Pro Ala Ile Ala Pro Ile Ile Ile Asp Val
405 410 415 Cys Lys Lys Tyr Asn Ile Lys Tyr Thr Val Leu Pro Thr Phe
Thr Glu 420 425 430 Ala Leu Leu Ala His Phe Lys His Leu Lys Asn Met
Gly Glu Leu Gly 435 440 445 Lys Pro Val Glu Ile His Met Gly 450 455
<210> SEQ ID NO 13 <211> LENGTH: 477 <212> TYPE:
PRT <213> ORGANISM: Phytophthora megasperma <220>
FEATURE: GenBank Accession No. CAD53323 <221> NAME/KEY:
MISC_FEATURE <223> OTHER INFORMATION: delta-5 desaturase
<400> SEQUENCE: 13 Met Ala Pro Ile Glu Thr Val Lys Asp Ala
Asn Glu Gly Leu His Gln 1 5 10 15 Arg Lys Gly Ala Ala Ala Ala Ser
Lys Asp Thr Thr Thr Phe Thr Trp 20 25 30 Gln Asp Val Ala Lys His
Asn Thr Ala Lys Ser Ala Trp Val Thr Ile 35 40 45 Arg Gly Val Val
Tyr Asp Val Thr Glu Trp Ala Asp Arg His Pro Gly 50 55 60 Gly Arg
Glu Leu Val Leu Leu His Ser Gly Arg Glu Cys Thr Asp Thr 65 70 75 80
Phe Asp Ser Tyr His Pro Phe Ser Asp Arg Ala Asp Lys Ile Leu Ala 85
90 95 Lys Tyr Ala Ile Gly Lys Leu Val Gly Gly Ser Glu Phe Pro Thr
Tyr 100 105 110 Lys Pro Asp Thr Gly Phe Tyr Lys Glu Cys Cys Asp Arg
Val Asn Gln 115 120 125 Tyr Phe Lys Asp Asn Lys Leu Asp Pro Arg Ser
Pro Tyr Ser Gly Leu 130 135 140 Trp Arg Met Ile Leu Val Ala Ile Val
Gly Ala Val Ala Tyr Met Gly 145 150 155 160 Met Asn Gln Leu Leu Pro
Gly Asn Ile Tyr Ala His Tyr Ala Trp Gly 165 170 175 Ala Leu Phe Gly
Val Cys Gln Ala Leu Pro Leu Leu His Val Met His 180 185 190 Asp Ala
Ser His Ala Ala Ile Thr Ser Ser Pro Thr Gly Trp Arg Leu 195 200 205
Ile Gly Arg Leu Ala Met Asp Trp Val Ala Gly Ala Asn Met Val Ser 210
215 220 Trp Leu Asn Gln His Val Val Gly His His Ile Tyr Thr Asn Val
Ala 225 230 235 240 Gly Ala Asp Pro Asp Leu Pro Val Asp Phe Lys Ser
Asp Val Arg Arg 245 250 255 Ile Val Tyr Arg Gln Val Leu Leu Pro Ile
Tyr Lys Tyr Gln His Leu 260 265 270 Tyr Leu Pro Pro Leu Tyr Gly Val
Leu Gly Leu Lys Phe Arg Val Gln 275 280 285 Asp Val Phe Glu Thr Phe
Val Thr Leu Thr Asn Gly Pro Leu Arg Val 290 295 300 Asn Pro Leu Ser
Val Gly Asp Trp Ala Glu Met Ile Leu Ser Lys Ala 305 310 315 320 Phe
Trp Val Phe Tyr Arg Ile Tyr Leu Pro Leu Ala Val Leu Gln Val 325 330
335 Asp Pro Ala Arg Phe Trp Gly Val Phe Phe Leu Ala Glu Phe Ser Thr
340 345 350 Gly Trp Tyr Leu Ala Phe Asn Phe Gln Val Ser His Val Ser
Thr Ala 355 360 365 Cys Glu Tyr Pro Gly Gly Asp Glu Glu Val Thr Ser
Ile Asp Asp Glu 370 375 380 Trp Ala Ile Ser Gln Val Lys Ser Ser Val
Asp Tyr Gly His Gly Ser 385 390 395 400 Phe Ile Thr Thr Phe Leu Thr
Gly Ala Leu Asn Tyr Gln Val Thr His 405 410 415 His Leu Phe Pro Gly
Val Ser Gln Tyr His Tyr Pro Ala Ile Ala Pro 420 425 430 Leu Ile Leu
Asp Val Cys His Lys Tyr Lys Val Lys Tyr Asn Val Leu 435 440 445 Pro
Asp Phe Thr Ala Ala Met Ala Gly His Phe Asp His Leu Val Ile 450 455
460 Met Gly Lys Met Gly Lys Arg Val Thr Ile His Met Gly 465 470 475
<210> SEQ ID NO 14 <211> LENGTH: 469 <212> TYPE:
PRT <213> ORGANISM: Phaeodactylum tricornutum <220>
FEATURE: GenBank Accession No. AAL92562 <221> NAME/KEY:
MISC_FEATURE <223> OTHER INFORMATION: delta-5 desaturase
<400> SEQUENCE: 14 Met Ala Pro Asp Ala Asp Lys Leu Arg Gln
Arg Gln Thr Thr Ala Val 1 5 10 15 Ala Lys His Asn Ala Ala Thr Ile
Ser Thr Gln Glu Arg Leu Cys Ser 20 25 30 Leu Ser Ser Leu Lys Gly
Glu Glu Val Cys Ile Asp Gly Ile Ile Tyr 35 40 45 Asp Leu Gln Ser
Phe Asp His Pro Gly Gly Glu Thr Ile Lys Met Phe 50 55 60 Gly Gly
Asn Asp Val Thr Val Gln Tyr Lys Met Ile His Pro Tyr His 65 70 75 80
Thr Glu Lys His Leu Glu Lys Met Lys Arg Val Gly Lys Val Thr Asp 85
90 95 Phe Val Cys Glu Tyr Lys Phe Asp Thr Glu Phe Glu Arg Glu Ile
Lys 100 105 110 Arg Glu Val Phe Lys Ile Val Arg Arg Gly Lys Asp Phe
Gly Thr Leu 115 120 125 Gly Trp Phe Phe Arg Ala Phe Cys Tyr Ile Ala
Ile Phe Phe Tyr Leu 130 135 140 Gln Tyr His Trp Val Thr Thr Gly Thr
Ser Trp Leu Leu Ala Val Ala 145 150 155 160 Tyr Gly Ile Ser Gln Ala
Met Ile Gly Met Asn Val Gln His Asp Ala 165 170 175 Asn His Gly Ala
Thr Ser Lys Arg Pro Trp Val Asn Asp Met Leu Gly 180 185 190 Leu Gly
Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Gln Glu Gln 195 200 205
His Trp Thr His His Ala Tyr Thr Asn His Ala Glu Met Asp Pro Asp 210
215 220 Ser Phe Gly Ala Glu Pro Met Leu Leu Phe Asn Asp Tyr Pro Leu
Asp 225 230 235 240 His Pro Ala Arg Thr Trp Leu His Arg Phe Gln Ala
Phe Phe Tyr Met 245 250 255 Pro Val Leu Ala Gly Tyr Trp Leu Ser Ala
Val Phe Asn Pro Gln Ile 260 265 270 Leu Asp Leu Gln Gln Arg Gly Ala
Leu Ser Val Gly Ile Arg Leu Asp 275 280 285 Asn Ala Phe Ile His Ser
Arg Arg Lys Tyr Ala Val Phe Trp Arg Ala 290 295 300 Val Tyr Ile Ala
Val Asn Val Ile Ala Pro Phe Tyr Thr Asn Ser Gly 305 310 315 320 Leu
Glu Trp Ser Trp Arg Val Phe Gly Asn Ile Met Leu Met Gly Val 325 330
335 Ala Glu Ser Leu Ala Leu Ala Val Leu Phe Ser Leu Ser His Asn Phe
340 345 350 Glu Ser Ala Asp Arg Asp Pro Thr Ala Pro Leu Lys Lys Thr
Gly Glu 355 360 365 Pro Val Asp Trp Phe Lys Thr Gln Val Glu Thr Ser
Cys Thr Tyr Gly 370 375 380 Gly Phe Leu Ser Gly Cys Phe Thr Gly Gly
Leu Asn Phe Gln Val Glu 385 390 395 400 His His Leu Phe Pro Arg Met
Ser Ser Ala Trp Tyr Pro Tyr Ile Ala 405 410 415 Pro Lys Val Arg Glu
Ile Cys Ala Lys His Gly Val His Tyr Ala Tyr 420 425 430 Tyr Pro Trp
Ile His Gln Asn Phe Leu Ser Thr Val Arg Tyr Met His 435 440 445 Ala
Ala Gly Thr Gly Ala Asn Trp Arg Gln Met Ala Arg Glu Asn Pro 450 455
460 Leu Thr Gly Arg Ala 465 <210> SEQ ID NO 15 <211>
LENGTH: 467 <212> TYPE: PRT <213> ORGANISM:
Dictyostelium discoideum <220> FEATURE: GenBank Accession No.
XP_640331 <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: delta-5 desaturase <400> SEQUENCE: 15 Met Met
Glu Thr Asn Asn Glu Asn Lys Glu Lys Leu Lys Leu Tyr Thr
1 5 10 15 Trp Asp Glu Val Ser Lys His Asn Gln Lys Asn Asp Leu Trp
Ile Ile 20 25 30 Val Asp Gly Lys Val Tyr Asn Ile Thr Lys Trp Val
Pro Leu His Pro 35 40 45 Gly Gly Glu Asp Ile Leu Leu Leu Ser Ala
Gly Arg Asp Ala Thr Asn 50 55 60 Leu Phe Glu Ser Tyr His Pro Met
Thr Asp Lys His Tyr Ser Leu Ile 65 70 75 80 Lys Gln Tyr Glu Ile Gly
Tyr Ile Ser Ser Tyr Glu His Pro Lys Tyr 85 90 95 Val Glu Lys Ser
Glu Phe Tyr Ser Thr Leu Lys Gln Arg Val Arg Lys 100 105 110 His Phe
Gln Thr Ser Ser Gln Asp Pro Lys Val Ser Val Gly Val Phe 115 120 125
Thr Arg Met Val Leu Ile Tyr Leu Phe Leu Phe Val Thr Tyr Tyr Leu 130
135 140 Ser Gln Phe Ser Thr Asp Arg Phe Trp Leu Asn Cys Ile Phe Ala
Val 145 150 155 160 Leu Tyr Gly Val Ala Asn Ser Leu Phe Gly Leu His
Thr Met His Asp 165 170 175 Ala Cys His Thr Ala Ile Thr His Asn Pro
Met Thr Trp Lys Ile Leu 180 185 190 Gly Ala Thr Phe Asp Leu Phe Ala
Gly Ala Ser Phe Tyr Ala Trp Cys 195 200 205 His Gln His Val Ile Gly
His His Leu Tyr Thr Asn Val Arg Asn Ala 210 215 220 Asp Pro Asp Leu
Gly Gln Gly Glu Ile Asp Phe Arg Val Val Thr Pro 225 230 235 240 Tyr
Gln Ala Arg Ser Trp Tyr His Lys Tyr Gln His Ile Tyr Ala Pro 245 250
255 Ile Leu Tyr Gly Val Tyr Ala Leu Lys Tyr Arg Ile Gln Asp His Glu
260 265 270 Ile Phe Thr Lys Lys Ser Asn Gly Ala Ile Arg Tyr Ser Pro
Ile Ser 275 280 285 Thr Ile Asp Thr Ala Ile Phe Ile Leu Gly Lys Leu
Val Phe Ile Ile 290 295 300 Ser Arg Phe Ile Leu Pro Leu Ile Tyr Asn
His Ser Phe Ser His Leu 305 310 315 320 Ile Cys Phe Phe Leu Ile Ser
Glu Leu Val Leu Gly Trp Tyr Leu Ala 325 330 335 Ile Ser Phe Gln Val
Ser His Val Val Glu Asp Leu Gln Phe Met Ala 340 345 350 Thr Pro Glu
Ile Phe Asp Gly Ala Asp His Pro Leu Pro Thr Thr Phe 355 360 365 Asn
Gln Asp Trp Ala Ile Leu Gln Val Lys Thr Thr Gln Asp Tyr Ala 370 375
380 Gln Asp Ser Val Leu Ser Thr Phe Phe Ser Gly Gly Leu Asn Leu Gln
385 390 395 400 Val Ile His His Cys Phe Pro Thr Ile Ala Gln Asp Tyr
Tyr Pro Gln 405 410 415 Ile Val Pro Ile Leu Lys Glu Val Cys Lys Glu
Tyr Asn Val Thr Tyr 420 425 430 His Tyr Lys Pro Thr Phe Thr Glu Ala
Ile Lys Ser His Ile Asn Tyr 435 440 445 Leu Tyr Lys Met Gly Asn Asp
Pro Asp Tyr Val Arg Lys Pro Val Asn 450 455 460 Lys Asn Asp 465
<210> SEQ ID NO 16 <211> LENGTH: 421 <212> TYPE:
PRT <213> ORGANISM: Euglena gracilis <220> FEATURE:
<221> NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION:
delta-8 desaturase <300> PUBLICATION INFORMATION: <302>
TITLE: DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED
FATTY ACIDS <310> PATENT DOCUMENT NUMBER: WO2006012325
<311> PATENT FILING DATE: 2005-06-24 <312> PUBLICATION
DATE: 2006-02-02 <313> RELEVANT RESIDUES IN SEQ ID NO:
(1)..(421) <300> PUBLICATION INFORMATION: <302> TITLE:
DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY
ACIDS <310> PATENT DOCUMENT NUMBER: WO2006012326 <311>
PATENT FILING DATE: 2005-06-24 <312> PUBLICATION DATE:
2006-02-02 <313> RELEVANT RESIDUES IN SEQ ID NO: (1)..(421)
<400> SEQUENCE: 16 Met Lys Ser Lys Arg Gln Ala Leu Pro Leu
Thr Ile Asp Gly Thr Thr 1 5 10 15 Tyr Asp Val Ser Ala Trp Val Asn
Phe His Pro Gly Gly Ala Glu Ile 20 25 30 Ile Glu Asn Tyr Gln Gly
Arg Asp Ala Thr Asp Ala Phe Met Val Met 35 40 45 His Ser Gln Glu
Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn 50 55 60 Pro Ser
Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu 65 70 75 80
Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp 85
90 95 Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly
Leu 100 105 110 Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met
Tyr Phe Ile 115 120 125 Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln
Met Gly Trp Leu Ser 130 135 140 His Asp Ile Cys His His Gln Thr Phe
Lys Asn Arg Asn Trp Asn Asn 145 150 155 160 Leu Val Gly Leu Val Phe
Gly Asn Gly Leu Gln Gly Phe Ser Val Thr 165 170 175 Trp Trp Lys Asp
Arg His Asn Ala His His Ser Ala Thr Asn Val Gln 180 185 190 Gly His
Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu 195 200 205
Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe 210
215 220 Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile
Trp 225 230 235 240 Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys
Asp Arg Asp Asn 245 250 255 Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu
Ala Ile Gly Leu Ala Leu 260 265 270 His Trp Thr Leu Lys Thr Leu Phe
His Leu Phe Phe Met Pro Ser Ile 275 280 285 Leu Thr Ser Leu Leu Val
Phe Phe Val Ser Glu Leu Val Gly Gly Phe 290 295 300 Gly Ile Ala Ile
Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile 305 310 315 320 Gly
Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His 325 330
335 Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly
340 345 350 Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu
Pro Arg 355 360 365 His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln
Leu Cys Gln Lys 370 375 380 His Asn Leu Pro Tyr Arg Asn Pro Leu Pro
His Glu Gly Leu Val Ile 385 390 395 400 Leu Leu Arg Tyr Leu Ala Val
Phe Ala Arg Met Ala Glu Lys Gln Pro 405 410 415 Ala Gly Lys Ala Leu
420 <210> SEQ ID NO 17 <211> LENGTH: 1269 <212>
TYPE: DNA <213> ORGANISM: Pavlova lutheri <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: delta-8 desaturase <400> SEQUENCE: 17 atgggcaagg
gtggagacgg cggcgcgcag gcggtgagcg ggaccgacgc gtctctcgct 60
gaggtgagct ccgtcgatag caagagcgtg cacgtcgtgc tctacggcaa gcgcgtggat
120 gtcacaaagt tccagaaggc acacccgggc gggagcaagg tgttccgcat
cttccaggag 180 cgcgacgcga cggagcagtt cgagtcttac cactcgccca
aggccatcaa gatgatggag 240 ggcatgctca agaagtcgga ggatgcgccc
gcttccgtgc ccctgccctc gcggtccacc 300 atgggcacgg agttcaagga
gatgattgag cgccacaaga gggctggtct ctacgaccct 360 tgcccgttgg
acgagctgtt caagctcacc atcgtccttg cgcccatctt cgtgggcgcc 420
tatctcgtgc ggagcggcgt ctcgcccctc gcgggcgcgc tctccatggg ctttggcttc
480 tacctcgacg gctggcttgc tcacgactac ctgcatcacg cagtcttcaa
gggctcggtc 540 aacacgctcg tcaaggcgaa caacgccatg ggatacgccc
tcggcttcct ccagggctac 600 gacgtggcct ggtggcgcgc gcgccataac
acgcaccacg tgtgcaccaa cgaggatggt 660 tcggacccgg acatcaagac
ggcgcccctg ctcatctacg tgcgagagaa cccgtccatt 720 gccaagcggc
tcaacttctt ccagcgctgg cagcagtact actatgtgcc gaccatggcc 780
atcctcgacc tctactggcg cctggagtcc atcgcgtacg tggctgtgcg cctgcctaag
840 atgtggatgc aggccgccgc tcttgccgct cactacgcgc tcctgtgctg
ggtcttcgca 900 gcgcatctca acctcatccc tctcatgatg gttgcacgcg
gcttcgcgac gggcatcgtt 960 gtctttgcaa cccactatgg tgaggacatc
ctcgaccgcg agcacgtcga gggcatgacg 1020 ctcgtcgagc agaccgccaa
gacctcccgt aacatcacgg gcggctggct agtgaacgtg 1080 ctcacgggct
tcatctccct gcagaccgag catcacctct tccccatgat gcccaccggc 1140
aacctaatga ctatccagcc cgaggtacgc gacttcttca agaagcatgg cctcgagtac
1200 cgcgagggca acctcttcca gtgcgtgcac cagaacatca aggctctcgc
cttcgagcac 1260 ctcctccac 1269
<210> SEQ ID NO 18 <211> LENGTH: 423 <212> TYPE:
PRT <213> ORGANISM: Pavlova lutheri <400> SEQUENCE: 18
Met Gly Lys Gly Gly Asp Gly Gly Ala Gln Ala Val Ser Gly Thr Asp 1 5
10 15 Ala Ser Leu Ala Glu Val Ser Ser Val Asp Ser Lys Ser Val His
Val 20 25 30 Val Leu Tyr Gly Lys Arg Val Asp Val Thr Lys Phe Gln
Lys Ala His 35 40 45 Pro Gly Gly Ser Lys Val Phe Arg Ile Phe Gln
Glu Arg Asp Ala Thr 50 55 60 Glu Gln Phe Glu Ser Tyr His Ser Pro
Lys Ala Ile Lys Met Met Glu 65 70 75 80 Gly Met Leu Lys Lys Ser Glu
Asp Ala Pro Ala Ser Val Pro Leu Pro 85 90 95 Ser Arg Ser Thr Met
Gly Thr Glu Phe Lys Glu Met Ile Glu Arg His 100 105 110 Lys Arg Ala
Gly Leu Tyr Asp Pro Cys Pro Leu Asp Glu Leu Phe Lys 115 120 125 Leu
Thr Ile Val Leu Ala Pro Ile Phe Val Gly Ala Tyr Leu Val Arg 130 135
140 Ser Gly Val Ser Pro Leu Ala Gly Ala Leu Ser Met Gly Phe Gly Phe
145 150 155 160 Tyr Leu Asp Gly Trp Leu Ala His Asp Tyr Leu His His
Ala Val Phe 165 170 175 Lys Gly Ser Val Asn Thr Leu Val Lys Ala Asn
Asn Ala Met Gly Tyr 180 185 190 Ala Leu Gly Phe Leu Gln Gly Tyr Asp
Val Ala Trp Trp Arg Ala Arg 195 200 205 His Asn Thr His His Val Cys
Thr Asn Glu Asp Gly Ser Asp Pro Asp 210 215 220 Ile Lys Thr Ala Pro
Leu Leu Ile Tyr Val Arg Glu Asn Pro Ser Ile 225 230 235 240 Ala Lys
Arg Leu Asn Phe Phe Gln Arg Trp Gln Gln Tyr Tyr Tyr Val 245 250 255
Pro Thr Met Ala Ile Leu Asp Leu Tyr Trp Arg Leu Glu Ser Ile Ala 260
265 270 Tyr Val Ala Val Arg Leu Pro Lys Met Trp Met Gln Ala Ala Ala
Leu 275 280 285 Ala Ala His Tyr Ala Leu Leu Cys Trp Val Phe Ala Ala
His Leu Asn 290 295 300 Leu Ile Pro Leu Met Met Val Ala Arg Gly Phe
Ala Thr Gly Ile Val 305 310 315 320 Val Phe Ala Thr His Tyr Gly Glu
Asp Ile Leu Asp Arg Glu His Val 325 330 335 Glu Gly Met Thr Leu Val
Glu Gln Thr Ala Lys Thr Ser Arg Asn Ile 340 345 350 Thr Gly Gly Trp
Leu Val Asn Val Leu Thr Gly Phe Ile Ser Leu Gln 355 360 365 Thr Glu
His His Leu Phe Pro Met Met Pro Thr Gly Asn Leu Met Thr 370 375 380
Ile Gln Pro Glu Val Arg Asp Phe Phe Lys Lys His Gly Leu Glu Tyr 385
390 395 400 Arg Glu Gly Asn Leu Phe Gln Cys Val His Gln Asn Ile Lys
Ala Leu 405 410 415 Ala Phe Glu His Leu Leu His 420 <210> SEQ
ID NO 19 <211> LENGTH: 7 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Conserved region #1 within delta-5 and delta-8
desaturases <220> FEATURE: <221> NAME/KEY: MISC_FEATURE
<222> LOCATION: (4)..(4) <223> OTHER INFORMATION: X =
Ile or Val <400> SEQUENCE: 19 Gly His His Xaa Tyr Thr Asn 1 5
<210> SEQ ID NO 20 <211> LENGTH: 7 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Conserved region #2 within delta-5
and delta-8 desaturases <220> FEATURE: <221> NAME/KEY:
MISC_FEATURE <222> LOCATION: (2)..(2) <223> OTHER
INFORMATION: X = Y or F <220> FEATURE: <221> NAME/KEY:
MISC_FEATURE <222> LOCATION: (4)..(4) <223> OTHER
INFORMATION: X = Val or Ile <400> SEQUENCE: 20 Asn Xaa Gln
Xaa Glu His His 1 5 <210> SEQ ID NO 21 <211> LENGTH:
476 <212> TYPE: PRT <213> ORGANISM: Thalassiosira
pseudonana <220> FEATURE: GenBank Accession No. AAX14502
<221> NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION:
delta-8 desaturase <400> SEQUENCE: 21 Met Pro Pro Asn Ala Asp
Ile Ser Arg Ile Arg Asn Arg Ile Pro Thr 1 5 10 15 Lys Thr Gly Thr
Val Ala Ser Ala Asp Asn Asn Asp Pro Ala Thr Gln 20 25 30 Ser Val
Arg Thr Leu Lys Ser Leu Lys Gly Asn Glu Val Val Ile Asn 35 40 45
Gly Thr Ile Tyr Asp Ile Ala Asp Phe Val His Pro Gly Gly Glu Val 50
55 60 Val Lys Phe Phe Gly Gly Asn Asp Val Thr Ile Gln Tyr Asn Met
Ile 65 70 75 80 His Pro Tyr His Thr Gly Lys His Leu Glu Lys Met Lys
Ala Val Gly 85 90 95 Lys Val Val Asp Trp Gln Ser Asp Tyr Lys Phe
Asp Thr Pro Phe Glu 100 105 110 Arg Glu Ile Lys Ser Glu Val Phe Lys
Ile Val Arg Arg Gly Arg Glu 115 120 125 Phe Gly Thr Thr Gly Tyr Phe
Leu Arg Ala Phe Phe Tyr Ile Ala Leu 130 135 140 Phe Phe Thr Met Gln
Tyr Thr Phe Ala Thr Cys Thr Thr Phe Thr Thr 145 150 155 160 Tyr Asp
His Trp Tyr Gln Ser Gly Val Phe Ile Ala Ile Val Phe Gly 165 170 175
Ile Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn His 180
185 190 Gly Ala Ala Ser Lys Arg Pro Trp Val Asn Asp Leu Leu Gly Phe
Gly 195 200 205 Thr Asp Leu Ile Gly Ser Asn Lys Trp Asn Trp Met Ala
Gln His Trp 210 215 220 Thr His His Ala Tyr Thr Asn His Ser Glu Lys
Asp Pro Asp Ser Phe 225 230 235 240 Ser Ser Glu Pro Met Phe Ala Phe
Asn Asp Tyr Pro Ile Gly His Pro 245 250 255 Lys Arg Lys Trp Trp His
Arg Phe Gln Gly Gly Tyr Phe Leu Phe Met 260 265 270 Leu Gly Leu Tyr
Trp Leu Pro Thr Val Phe Asn Pro Gln Phe Ile Asp 275 280 285 Leu Arg
Gln Arg Gly Ala Gln Tyr Val Gly Ile Gln Met Glu Asn Asp 290 295 300
Phe Ile Val Lys Arg Arg Lys Tyr Ala Val Ala Leu Arg Met Met Tyr 305
310 315 320 Ile Tyr Leu Asn Ile Val Ser Pro Phe Met Asn Asn Gly Leu
Ser Trp 325 330 335 Ser Thr Phe Gly Ile Ile Met Leu Met Gly Ile Ser
Glu Ser Leu Thr 340 345 350 Leu Ser Val Leu Phe Ser Leu Ser His Asn
Phe Ile Asn Ser Asp Arg 355 360 365 Asp Pro Thr Ala Asp Phe Lys Lys
Thr Gly Glu Gln Val Cys Trp Phe 370 375 380 Lys Ser Gln Val Glu Thr
Ser Ser Thr Tyr Gly Gly Phe Ile Ser Gly 385 390 395 400 Cys Leu Thr
Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe Pro 405 410 415 Arg
Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg Glu 420 425
430 Val Cys Lys Lys His Gly Met Ser Tyr Ala Tyr Tyr Pro Trp Ile Gly
435 440 445 Gln Asn Leu Val Ser Thr Phe Lys Tyr Met His Arg Ala Gly
Ser Gly 450 455 460 Ala Asn Trp Glu Leu Lys Pro Leu Ser Gly Ser Ala
465 470 475 <210> SEQ ID NO 22 <211> LENGTH: 8165
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid pZUF17
<400> SEQUENCE: 22 gtacgagccg gaagcataaa gtgtaaagcc
tggggtgcct aatgagtgag ctaactcaca 60 ttaattgcgt tgcgctcact
gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 120 taatgaatcg
gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 180
tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
240
aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
300 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
tttccatagg 360 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
gtcagaggtg gcgaaacccg 420 acaggactat aaagatacca ggcgtttccc
cctggaagct ccctcgtgcg ctctcctgtt 480 ccgaccctgc cgcttaccgg
atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 540 tctcatagct
cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 600
tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
660 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
taacaggatt 720 agcagagcga ggtatgtagg cggtgctaca gagttcttga
agtggtggcc taactacggc 780 tacactagaa ggacagtatt tggtatctgc
gctctgctga agccagttac cttcggaaaa 840 agagttggta gctcttgatc
cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 900 tgcaagcagc
agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 960
acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1020 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
atcaatctaa 1080 agtatatatg agtaaacttg gtctgacagt taccaatgct
taatcagtga ggcacctatc 1140 tcagcgatct gtctatttcg ttcatccata
gttgcctgac tccccgtcgt gtagataact 1200 acgatacggg agggcttacc
atctggcccc agtgctgcaa tgataccgcg agacccacgc 1260 tcaccggctc
cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 1320
ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
1380 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
catcgtggtg 1440 tcacgctcgt cgtttggtat ggcttcattc agctccggtt
cccaacgatc aaggcgagtt 1500 acatgatccc ccatgttgtg caaaaaagcg
gttagctcct tcggtcctcc gatcgttgtc 1560 agaagtaagt tggccgcagt
gttatcactc atggttatgg cagcactgca taattctctt 1620 actgtcatgc
catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 1680
tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
1740 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
ggggcgaaaa 1800 ctctcaagga tcttaccgct gttgagatcc agttcgatgt
aacccactcg tgcacccaac 1860 tgatcttcag catcttttac tttcaccagc
gtttctgggt gagcaaaaac aggaaggcaa 1920 aatgccgcaa aaaagggaat
aagggcgaca cggaaatgtt gaatactcat actcttcctt 1980 tttcaatatt
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 2040
tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
2100 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
cagcgtgacc 2160 gctacacttg ccagcgccct agcgcccgct cctttcgctt
tcttcccttc ctttctcgcc 2220 acgttcgccg gctttccccg tcaagctcta
aatcgggggc tccctttagg gttccgattt 2280 agtgctttac ggcacctcga
ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 2340 ccatcgccct
gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2400
ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
2460 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
acaaaaattt 2520 aacgcgaatt ttaacaaaat attaacgctt acaatttcca
ttcgccattc aggctgcgca 2580 actgttggga agggcgatcg gtgcgggcct
cttcgctatt acgccagctg gcgaaagggg 2640 gatgtgctgc aaggcgatta
agttgggtaa cgccagggtt ttcccagtca cgacgttgta 2700 aaacgacggc
cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc 2760
ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct
2820 tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt
ctacactgat 2880 taattttcgg gccaataatt taaaaaaatc gtgttatata
atattatatg tattatatat 2940 atacatcatg atgatactga cagtcatgtc
ccattgctaa atagacagac tccatctgcc 3000 gcctccaact gatgttctca
atatttaagg ggtcatctcg cattgtttaa taataaacag 3060 actccatcta
ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt 3120
acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa
3180 tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat
aaatgcgtat 3240 gggaaatctt aaatatggat agcataaatg atatctgcat
tgcctaattc gaaatcaaca 3300 gcaacgaaaa aaatcccttg tacaacataa
atagtcatcg agaaatatca actatcaaag 3360 aacagctatt cacacgttac
tattgagatt attattggac gagaatcaca cactcaactg 3420 tctttctctc
ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct 3480
agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca
3540 aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc
aaaaagcttc 3600 tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc
attaaaggta tatatttatt 3660 tcttgttata taatcctttt gtttattaca
tgggctggat acataaaggt attttgattt 3720 aattttttgc ttaaattcaa
tcccccctcg ttcagtgtca actgtaatgg taggaaatta 3780 ccatactttt
gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga 3840
cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg
3900 ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta
caactatgta 3960 ctactgttga tgcatccaca acagtttgtt ttgttttttt
ttgttttttt tttttctaat 4020 gattcattac cgctatgtat acctacttgt
acttgtagta agccgggtta ttggcgttca 4080 attaatcata gacttatgaa
tctgcacggt gtgcgctgcg agttactttt agcttatgca 4140 tgctacttgg
gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg 4200
acagtaatta attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt
4260 agttcaacgt attagcactg tacccagcat ctccgtatcg agaaacacaa
caacatgccc 4320 cattggacag atcatgcgga tacacaggtt gtgcagtatc
atacatactc gatcagacag 4380 gtcgtctgac catcatacaa gctgaacaag
cgctccatac ttgcacgctc tctatataca 4440 cagttaaatt acatatccat
agtctaacct ctaacagtta atcttctggt aagcctccca 4500 gccagccttc
tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc 4560
tcggccgaca attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg
4620 ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa
taagccagtc 4680 ctcagagtcg cccttaggtc ggttctgggc aatgaagcca
accacaaact cggggtcgga 4740 tcgggcaagc tcaatggtct gcttggagta
ctcgccagtg gccagagagc ccttgcaaga 4800 cagctcggcc agcatgagca
gacctctggc cagcttctcg ttgggagagg ggactaggaa 4860 ctccttgtac
tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt 4920
ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt
4980 ggtgatatcg gaccactcgg cgattcggtg acaccggtac tggtgcttga
cagtgttgcc 5040 aatatctgcg aactttctgt cctcgaacag gaagaaaccg
tgcttaagag caagttcctt 5100 gagggggagc acagtgccgg cgtaggtgaa
gtcgtcaatg atgtcgatat gggttttgat 5160 catgcacaca taaggtccga
ccttatcggc aagctcaatg agctccttgg tggtggtaac 5220 atccagagaa
gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc 5280
aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag
5340 gagactgaaa taaatttagt ctgcagaact ttttatcgga accttatctg
gggcagtgaa 5400 gtatatgtta tggtaatagt tacgagttag ttgaacttat
agatagactg gactatacgg 5460 ctatcggtcc aaattagaaa gaacgtcaat
ggctctctgg gcgtcgcctt tgccgacaaa 5520 aatgtgatca tgatgaaagc
cagcaatgac gttgcagctg atattgttgt cggccaaccg 5580 cgccgaaaac
gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat 5640
ccaagcacac tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata
5700 ctcgtcgact caggcgacga cggaattcct gcagcccatc tgcagaattc
aggagagacc 5760 gggttggcgg cgtatttgtg tcccaaaaaa cagccccaat
tgccccggag aagacggcca 5820 ggccgcctag atgacaaatt caacaactca
cagctgactt tctgccattg ccactagggg 5880 ggggcctttt tatatggcca
agccaagctc tccacgtcgg ttgggctgca cccaacaata 5940 aatgggtagg
gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg 6000
gggctcaatg gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga
6060 caccattgca tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct
gatctggaca 6120 ccacagaggt tccgagcact ttaggttgca ccaaatgtcc
caccaggtgc aggcagaaaa 6180 cgctggaaca gcgtgtacag tttgtcttaa
caaaaagtga gggcgctgag gtcgagcagg 6240 gtggtgtgac ttgttatagc
ctttagagct gcgaaagcgc gtatggattt ggctcatcag 6300 gccagattga
gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata 6360
tagccccgac aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg
6420 tacccacacc ttgcttctcc tgcacttgcc aaccttaata ctggtttaca
ttgaccaaca 6480 tcttacaagc ggggggcttg tctagggtat atataaacag
tggctctccc aatcggttgc 6540 cagtctcttt tttcctttct ttccccacag
attcgaaatc taaactacac atcacacaat 6600 gcctgttact gacgtcctta
agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc 6660 cgtgagtatc
cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc 6720
gaaagtcgct agcaacacac actctctaca caaactaacc cagctctcca tggctgagga
6780 taagaccaag gtcgagttcc ctaccctgac tgagctgaag cactctatcc
ctaacgcttg 6840 ctttgagtcc aacctcggac tctcgctcta ctacactgcc
cgagcgatct tcaacgcatc 6900 tgcctctgct gctctgctct acgctgcccg
atctactccc ttcattgccg ataacgttct 6960 gctccacgct ctggtttgcg
ccacctacat ctacgtgcag ggtgtcatct tctggggttt 7020 ctttaccgtc
ggtcacgact gtggtcactc tgccttctcc cgataccact ccgtcaactt 7080
catcattggc tgcatcatgc actctgccat tctgactccc ttcgagtcct ggcgagtgac
7140 ccaccgacac catcacaaga acactggcaa cattgataag gacgagatct
tctaccctca 7200 tcggtccgtc aaggacctcc aggacgtgcg acaatgggtc
tacaccctcg gaggtgcttg 7260 gtttgtctac ctgaaggtcg gatatgctcc
tcgaaccatg tcccactttg acccctggga 7320 ccctctcctg cttcgacgag
cctccgctgt catcgtgtcc ctcggagtct gggctgcctt 7380 cttcgctgcc
tacgcctacc tcacatactc gctcggcttt gccgtcatgg gcctctacta 7440
ctatgctcct ctctttgtct ttgcttcgtt cctcgtcatt actaccttct tgcatcacaa
7500 cgacgaagct actccctggt acggtgactc ggagtggacc tacgtcaagg
gcaacctgag 7560 ctccgtcgac cgatcgtacg gagctttcgt ggacaacctg
tctcaccaca ttggcaccca 7620 ccaggtccat cacttgttcc ctatcattcc
ccactacaag ctcaacgaag ccaccaagca 7680 ctttgctgcc gcttaccctc
acctcgtgag acgtaacgac gagcccatca ttactgcctt 7740 cttcaagacc
gctcacctct ttgtcaacta cggagctgtg cccgagactg ctcagatttt 7800
caccctcaaa gagtctgccg ctgcagccaa ggccaagagc gactaagcgg ccgcaagtgt
7860 ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga
tggatggatt 7920 caacacaggg atatagcgag ctacgtggtg gtgcgaggat
atagcaacgg atatttatgt 7980 ttgacacttg agaatgtacg atacaagcac
tgtccaagta caatactaaa catactgtac 8040 atactcatac tcgtacccgg
gcaacggttt cacttgagtg cagtggctag tgctcttact 8100 cgtacagtgt
gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta 8160 gttgc
8165 <210> SEQ ID NO 23 <211> LENGTH: 8438 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pDMW367 <400>
SEQUENCE: 23 catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg
ttgcgattga 60 tggcatcctc tacgaccttg aagggcttgc caaagttcat
ccaggaggag atttgattct 120 cgcttctggt gcctctgatg cctcccctct
cttttattca atgcatccat acgtcaaacc 180 ggagaattcc aaattgcttc
aacagttcgt ccgagggaag catgaccgca cctcgaagga 240 cattgtctac
acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt 300
gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat
360 cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc
tgtggggcct 420 gttgactgga ttcatgcaca tgcagatcgg cttatccatc
cagcatgatg cgtcccacgg 480 ggccatcagc aagaagcctt gggtcaacgc
cctcttcgcc tacggcattg acgtcatcgg 540 atcgtcccgg tggatttggc
tgcagtcgca catcatgcgg caccacacct acaccaacca 600 gcacggcctc
gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc 660
cgccgcaaac accgcccgaa agtggttcca ccgcttccaa gcttggtaca tgtaccttgt
720 gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc
ggatgcagca 780 caatgacacc atcccagagt ctgtcacggc catgcgggaa
aatggctttc tgcggcgcta 840 ccgcacactt gcattcgtga tgcgagcttt
cttcatcttc cggaccgcat tcttgccctg 900 gtacctcact gggacctcat
tgctgatcac cattcctctg gtgcccaccg caactggtgc 960 cttcttgacg
ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga 1020
caagaactgc aaggttaaga gatctgagaa ggacgttgag gctgaccaaa ttgactggta
1080 tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt
tcttcactgg 1140 cggtctcaat ttccagatcg agcaccacct ctttccccgg
atgtcgtctt ggcactaccc 1200 cttcgtccag caggcggtcc gggagtgttg
cgaacgccat ggagtgcgat atgttttcta 1260 ccctaccatc gtcggcaaca
tcatctccac cctgaagtac atgcataagg tgggtgtcgt 1320 ccactgcgtg
aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag 1380
tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata
1440 gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac
acttgagaat 1500 gtacgataca agcactgtcc aagtacaata ctaaacatac
tgtacatact catactcgta 1560 cccgggcaac ggtttcactt gagtgcagtg
gctagtgctc ttactcgtac agtgtgcaat 1620 actgcgtatc atagtctttg
atgtatatcg tattcattca tgttagttgc gtacgagccg 1680 gaagcataaa
gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 1740
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
1800 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
tcgctcactg 1860 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
agctcactca aaggcggtaa 1920 tacggttatc cacagaatca ggggataacg
caggaaagaa catgtgagca aaaggccagc 1980 aaaaggccag gaaccgtaaa
aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2040 ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2100
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
2160 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
tctcatagct 2220 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
caagctgggc tgtgtgcacg 2280 aaccccccgt tcagcccgac cgctgcgcct
tatccggtaa ctatcgtctt gagtccaacc 2340 cggtaagaca cgacttatcg
ccactggcag cagccactgg taacaggatt agcagagcga 2400 ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2460
ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2520 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
tgcaagcagc 2580 agattacgcg cagaaaaaaa ggatctcaag aagatccttt
gatcttttct acggggtctg 2640 acgctcagtg gaacgaaaac tcacgttaag
ggattttggt catgagatta tcaaaaagga 2700 tcttcaccta gatcctttta
aattaaaaat gaagttttaa atcaatctaa agtatatatg 2760 agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 2820
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
2880 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
tcaccggctc 2940 cagatttatc agcaataaac cagccagccg gaagggccga
gcgcagaagt ggtcctgcaa 3000 ctttatccgc ctccatccag tctattaatt
gttgccggga agctagagta agtagttcgc 3060 cagttaatag tttgcgcaac
gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3120 cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3180
ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3240 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
actgtcatgc 3300 catccgtaag atgcttttct gtgactggtg agtactcaac
caagtcattc tgagaatagt 3360 gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc gcgccacata 3420 gcagaacttt aaaagtgctc
atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3480 tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3540
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3600 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
tttcaatatt 3660 attgaagcat ttatcagggt tattgtctca tgagcggata
catatttgaa tgtatttaga 3720 aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct gacgcgccct 3780 gtagcggcgc attaagcgcg
gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3840 ccagcgccct
agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3900
gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3960 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
ccatcgccct 4020 gatagacggt ttttcgccct ttgacgttgg agtccacgtt
ctttaatagt ggactcttgt 4080 tccaaactgg aacaacactc aaccctatct
cggtctattc ttttgattta taagggattt 4140 tgccgatttc ggcctattgg
ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4200 ttaacaaaat
attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga 4260
agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
4320 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
aaacgacggc 4380 cagtgaattg taatacgact cactataggg cgaattgggt
accgggcccc ccctcgaggt 4440 cgatggtgtc gataagcttg atatcgaatt
catgtcacac aaaccgatct tcgcctcaag 4500 gaaacctaat tctacatccg
agagactgcc gagatccagt ctacactgat taattttcgg 4560 gccaataatt
taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg 4620
atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact
4680 gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag
actccatcta 4740 ccgcctccaa atgatgttct caaaatatat tgtatgaact
tatttttatt acttagtatt 4800 attagacaac ttacttgctt tatgaaaaac
acttcctatt taggaaacaa tttataatgg 4860 cagttcgttc atttaacaat
ttatgtagaa taaatgttat aaatgcgtat gggaaatctt 4920 aaatatggat
agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa 4980
aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt
5040 cacacgttac tattgagatt attattggac gagaatcaca cactcaactg
tctttctctc 5100 ttctagaaat acaggtacaa gtatgtacta ttctcattgt
tcatacttct agtcatttca 5160 tcccacatat tccttggatt tctctccaat
gaatgacatt ctatcttgca aattcaacaa 5220 ttataataag atataccaaa
gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc 5280 ttctcgtatt
tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata 5340
taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc
5400 ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta
ccatactttt 5460 gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt
ccaggttaga cgttccgcag 5520 aatctagaat gcggtatgcg gtacattgtt
cttcgaacgt aaaagttgcg ctccctgaga 5580 tattgtacat ttttgctttt
acaagtacaa gtacatcgta caactatgta ctactgttga 5640 tgcatccaca
acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac 5700
cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata
5760 gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca
tgctacttgg 5820 gtgtaatatt gggatctgtt cggaaatcaa cggatgctca
atcgatttcg acagtaatta 5880 attaagtcat acacaagtca gctttcttcg
agcctcatat aagtataagt agttcaacgt 5940 attagcactg tacccagcat
ctccgtatcg agaaacacaa caacatgccc cattggacag 6000 atcatgcgga
tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac 6060
catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt
6120 acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca
gccagccttc 6180 tggtatcgct tggcctcctc aataggatct cggttctggc
cgtacagacc tcggccgaca 6240 attatgatat ccgttccggt agacatgaca
tcctcaacag ttcggtactg ctgtccgaga 6300 gcgtctccct tgtcgtcaag
acccaccccg ggggtcagaa taagccagtc ctcagagtcg 6360 cccttaggtc
ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc 6420
tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc
6480 agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa
ctccttgtac 6540 tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt
cagagacagt ttcctcggca 6600 ccagctcgca ggccagcaat gattccggtt
ccgggtacac cgtgggcgtt ggtgatatcg 6660 gaccactcgg cgattcggtg
acaccggtac tggtgcttga cagtgttgcc aatatctgcg 6720 aactttctgt
cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc 6780
acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca
6840 taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac
atccagagaa 6900 gcacacaggt tggttttctt ggctgccacg agcttgagca
ctcgagcggc aaaggcggac 6960 ttgtggacgt tagctcgagc ttcgtaggag
ggcattttgg tggtgaagag gagactgaaa 7020 taaatttagt ctgcagaact
ttttatcgga accttatctg gggcagtgaa gtatatgtta 7080 tggtaatagt
tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc 7140
aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca
7200 tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg
cgccgaaaac 7260 gcagctgtca gacccacagc ctccaacgaa gaatgtatcg
tcaaagtgat ccaagcacac 7320 tcatagttgg agtcgtactc caaaggcggc
aatgacgagt cagacagata ctcgtcgact 7380 caggcgacga cggaattcct
gcagcccatc tgcagaattc aggagagacc gggttggcgg 7440 cgtatttgtg
tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag 7500
atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt
7560 tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata
aatgggtagg 7620 gttgcaccaa caaagggatg ggatgggggg tagaagatac
gaggataacg gggctcaatg 7680 gcacaaataa gaacgaatac tgccattaag
actcgtgatc cagcgactga caccattgca 7740 tcatctaagg gcctcaaaac
tacctcggaa ctgctgcgct gatctggaca ccacagaggt 7800 tccgagcact
ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca 7860
gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac
7920 ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag
gccagattga 7980 gggtctgtgg acacatgtca tgttagtgta cttcaatcgc
cccctggata tagccccgac 8040 aataggccgt ggcctcattt ttttgccttc
cgcacatttc cattgctcgg tacccacacc 8100 ttgcttctcc tgcacttgcc
aaccttaata ctggtttaca ttgaccaaca tcttacaagc 8160 ggggggcttg
tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt 8220
tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact
8280 gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc
cgtgagtatc 8340 cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat
gacacaatcc gaaagtcgct 8400 agcaacacac actctctaca caaactaacc
cagctctc 8438 <210> SEQ ID NO 24 <211> LENGTH: 12649
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid
pKUNF12T6E <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (2507)..(2507) <223> OTHER INFORMATION:
n is a, c, g, or t <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (2512)..(2515) <223> OTHER
INFORMATION: n is a, c, g, or t <400> SEQUENCE: 24 taaccctcac
taaagggaac aaaagctgga gctccaccgc ggacacaata tctggtcaaa 60
tttcagtttc gttacataaa tcgttatgtc aaaggagtgt gggaggttaa gagaattatc
120 accggcaaac tatctgttaa ttgctaggta cctctagacg tccacccggg
tcgcttggcg 180 gccgaagagg ccggaatctc gggccgcggt ggcggccgct
tagttggtct tggacttctt 240 gggcttcttc aggtaggact ggacaaagaa
gttgccgaac agagcgagca gggtgatcat 300 gtacacgccg agcagctgga
ccagagcctg agggtagtcg caggggaaga ggtagtcgta 360 cagggactgc
accagcatag ccatgaactg ggtcatctgc agagtggtga tgtagggctt 420
gatgggcttg acgaagccga agccctgaga ggaaaagaag tagtaggcgt acatgacggt
480 gtggacgaag gagttgagga tgacggagaa gtaggcgtcg ccaccaggag
cgtacttggc 540 aatagcccac cagatggcga agatggtggc atggtggtac
acgtgcagga aggagacctg 600 gttgaacttc ttgcacagga tcatgatagc
ggtgtccagg aactcgtagg ccttggagac 660 gtagaacacg tagacgattc
gggacatgcc ctgagcgtgg gactcgttgc ccttctccat 720 gtcgttgccg
aagaccttgt agccacccag gatagcctgt cggatggtct cgacgcacat 780
gtagagggac agtccgaaga ggaacaggtt gtggagcagc ttgatggtct tcagctcgaa
840 gggcttctcc atctgcttca tgatgggaat gccgaagagc agcatggcca
tgtagccgac 900 ctcgaaggcg agcatggtgg agacgtccat catgggcaga
ccgtcggtca gagcgtaggg 960 cttagctccg tccatccact ggtcgacacc
ggtctcgact cgtccgacca cgtcgtccca 1020 gacagaggag ttggccatgg
tgaatgattc ttatactcag aaggaaatgc ttaacgattt 1080 cgggtgtgag
ttgacaagga gagagagaaa agaagaggaa aggtaattcg gggacggtgg 1140
tcttttatac ccttggctaa agtcccaacc acaaagcaaa aaaattttca gtagtctatt
1200 ttgcgtccgg catgggttac ccggatggcc agacaaagaa actagtacaa
agtctgaaca 1260 agcgtagatt ccagactgca gtaccctacg cccttaacgg
caagtgtggg aaccggggga 1320 ggtttgatat gtggggtgaa gggggctctc
gccggggttg ggcccgctac tgggtcaatt 1380 tggggtcaat tggggcaatt
ggggctgttt tttgggacac aaatacgccg ccaacccggt 1440 ctctcctgaa
ttctgcatcg atcgaggaag aggacaagcg gctgcttctt aagtttgtga 1500
catcagtatc caaggcacca ttgcaaggat tcaaggcttt gaacccgtca tttgccattc
1560 gtaacgctgg tagacaggtt gatcggttcc ctacggcctc cacctgtgtc
aatcttctca 1620 agctgcctga ctatcaggac attgatcaac ttcggaagaa
acttttgtat gccattcgat 1680 cacatgctgg tttcgatttg tcttagagga
acgcatatac agtaatcata gagaataaac 1740 gatattcatt tattaaagta
gatagttgag gtagaagttg taaagagtga taaatagcgg 1800 ccgcgcctac
ttaagcaacg ggcttgataa cagcgggggg ggtgcccacg ttgttgcggt 1860
tgcggaagaa cagaacaccc ttaccagcac cctcggcacc agcgctgggc tcaacccact
1920 ggcacatacg cgcactgcgg tacatggcgc ggatgaagcc acgaggacca
tcctggacat 1980 cagcccggta gtgcttgccc atgatgggct taatggcctc
ggtggcctcg tccgcgttgt 2040 agaaggggat gctgctgacg tagtggtgga
ggacatgagt ctcgatgatg ccgtggagaa 2100 ggtggcggcc gatgaagccc
atctcacggt caatggtagc agcggcacca cggacgaagt 2160 tccactcgtc
gttggtgtag tggggaaggg tagggtcggt gtgctggagg aaggtgatgg 2220
caacgagcca gtggttaacc cagaggtagg gaacaaagta ccagatggcc atgttgtaga
2280 aaccgaactt ctgaacgagg aagtacagag cagtggccat cagaccgata
ccaatatcgc 2340 tgaggacgat gagcttagcg tcactgttct cgtacagagg
gctgcgggga tcgaagtggt 2400 taacaccacc gccgaggccg ttatgcttgc
ccttgccgcg accctcacgc tggcgctcgt 2460 ggtagttgtg gccggtaaca
ttggtgatga ggtagttggg ccagccnacg annnnctcag 2520 taagatgagc
gagctcgtgg gtcatctttc cgagacgagt agcctgctgc tcgcgggttc 2580
ggggaacgaa gaccatgtca cgctccatgt tgccagtggc cttgtggtgc tttcggtggg
2640 agatttgcca gctgaagtag gggacaagga gggaagagtg aagaacccag
ccagtaatgt 2700 cgttgatgat gcgagaatcg gagaaagcac cgtgaccgca
ctcatgggca ataacccaga 2760 gaccagtacc gaaaagaccc tgaagaacgg
tgtacacggc ccacagacca gcgcgggcgg 2820 gggtggaggg gatatattcg
ggggtcacaa agttgtacca gatgctgaaa gtggtagtca 2880 ggaggacaat
gtcgcggagg atataaccgt atcccttgag agcggagcgc ttgaagcagt 2940
gcttagggat ggcattgtag atgtccttga tggtaaagtc gggaacctcg aactggttgc
3000 cgtaggtgtc gagcatgaca ccatactcgg acttgggctt ggcgatatca
acctcggaca 3060 tggacgagag cgatgtggaa gaggccgagt ggcggggaga
gtctgaagga gagacggcgg 3120 cagactcaga atccgtcaca gtagttgagg
tgacggtgcg tctaagcgca gggttctgct 3180 tgggcagagc cgaagtggac
gccatggaga gctgggttag tttgtgtaga gagtgtgtgt 3240 tgctagcgac
tttcggattg tgtcattaca caaaacgcgt cgtctcgaca ctgatcttgt 3300
cgtggatact cacggctcgg acatcgtcgc cgacgatgac accggacttt cgcttaagga
3360 cgtcagtaac aggcattgtg tgatgtgtag tttagatttc gaatctgtgg
ggaaagaaag 3420 gaaaaaagag actggcaacc gattgggaga gccactgttt
atatataccc tagacaagcc 3480 ccccgcttgt aagatgttgg tcaatgtaaa
ccagtattaa ggttggcaag tgcaggagaa 3540 gcaaggtgtg ggtaccgagc
aatggaaatg tgcggaaggc aaaaaaatga ggccacggcc 3600 tattgtcggg
gctatatcca gggggcgatt gaagtacact aacatgacat gtgtccacag 3660
accctcaatc tggcctgatg agccaaatcc atacgcgctt tcgcagctct aaaggctata
3720 acaagtcaca ccaccctgct cgacctcagc gccctcactt tttgttaaga
caaactgtac 3780 acgctgttcc agcgttttct gcctgcacct ggtgggacat
ttggtgcaac ctaaagtgct 3840 cggaacctct gtggtgtcca gatcagcgca
gcagttccga ggtagttttg aggcccttag 3900 atgatgcaat ggtgtcagtc
gctggatcac gagtcttaat ggcagtattc gttcttattt 3960 gtgccattga
gccccgttat cctcgtatct tctacccccc atcccatccc tttgttggtg 4020
caaccctacc catttattgt tgggtgcagc ccaaccgacg tggagagctt ggcttggcca
4080 tataaaaagg ccccccccta gtggcaatgg cagaaagtca gctgtgagtt
gttgaatttg 4140 tcatctaggc ggcctggccg tcttctccgg ggcaattgtt
cctctatagt actgcgtaca 4200 ctgtttaaac agtgtacgca gatctgcgac
gacggaattc ctgcagccca tctgcagaat 4260 tcaggagaga ccgggttggc
ggcgtatttg tgtcccaaaa aacagcccca attgccccaa 4320 ttgaccccaa
attgacccag tagcgggccc aaccccggcg agagccccct tcaccccaca 4380
tatcaaacct cccccggttc ccacacttgc cgttaagggc gtagggtact gcagtctgga
4440 atctacgctt gttcagactt tgtactagtt tctttgtctg gccatccggg
taacccatgc 4500 cggacgcaaa atagactact gaaaattttt ttgctttgtg
gttgggactt tagccaaggg 4560 tataaaagac caccgtcccc gaattacctt
tcctcttctt ttctctctct ccttgtcaac 4620 tcacacccga aatcgttaag
catttccttc tgagtataag aatcattcac catggctgcc 4680 gctccctctg
tgcgaacctt tacccgagcc gaggttctga acgctgaggc tctgaacgag 4740
ggcaagaagg acgctgaggc tcccttcctg atgatcatcg acaacaaggt gtacgacgtc
4800 cgagagttcg tccctgacca tcctggaggc tccgtgattc tcacccacgt
tggcaaggac 4860 ggcaccgacg tctttgacac ctttcatccc gaggctgctt
gggagactct cgccaacttc 4920 tacgttggag acattgacga gtccgaccga
gacatcaaga acgatgactt tgccgctgag 4980 gtccgaaagc tgcgaaccct
gttccagtct ctcggctact acgactcctc taaggcctac 5040 tacgccttca
aggtctcctt caacctctgc atctggggac tgtccaccgt cattgtggcc 5100
aagtggggtc agacctccac cctcgccaac gtgctctctg ctgccctgct cggcctgttc
5160 tggcagcagt gcggatggct ggctcacgac tttctgcacc accaggtctt
ccaggaccga 5220 ttctggggtg atctcttcgg agccttcctg ggaggtgtct
gccagggctt ctcctcttcc 5280 tggtggaagg acaagcacaa cactcaccat
gccgctccca acgtgcatgg cgaggatcct 5340
gacattgaca cccaccctct cctgacctgg tccgagcacg ctctggagat gttctccgac
5400 gtccccgatg aggagctgac ccgaatgtgg tctcgattca tggtcctgaa
ccagacctgg 5460 ttctacttcc ccattctctc cttcgctcga ctgtcttggt
gcctccagtc cattctcttt 5520 gtgctgccca acggtcaggc tcacaagccc
tccggagctc gagtgcccat ctccctggtc 5580 gagcagctgt ccctcgccat
gcactggacc tggtacctcg ctaccatgtt cctgttcatc 5640 aaggatcctg
tcaacatgct cgtgtacttc ctggtgtctc aggctgtgtg cggaaacctg 5700
ctcgccatcg tgttctccct caaccacaac ggtatgcctg tgatctccaa ggaggaggct
5760 gtcgacatgg atttctttac caagcagatc atcactggtc gagatgtcca
tcctggactg 5820 ttcgccaact ggttcaccgg tggcctgaac taccagatcg
agcatcacct gttcccttcc 5880 atgcctcgac acaacttctc caagatccag
cctgccgtcg agaccctgtg caagaagtac 5940 aacgtccgat accacaccac
tggtatgatc gagggaactg ccgaggtctt ctcccgactg 6000 aacgaggtct
ccaaggccac ctccaagatg ggcaaggctc agtaagcggc cgcatgagaa 6060
gataaatata taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct
6120 cggagagaag ccaagacgag tactcaaagg ggattacacc atccatatcc
acagacacaa 6180 gctggggaaa ggttctatat acactttccg gaataccgta
gtttccgatg ttatcaatgg 6240 gggcagccag gatttcaggc acttcggtgt
ctcggggtga aatggcgttc ttggcctcca 6300 tcaagtcgta ccatgtcttc
atttgcctgt caaagtaaaa cagaagcaga tgaagaatga 6360 acttgaagtg
aaggaattta aattgccccg gagaagacgg ccaggccgcc tagatgacaa 6420
attcaacaac tcacagctga ctttctgcca ttgccactag gggggggcct ttttatatgg
6480 ccaagccaag ctctccacgt cggttgggct gcacccaaca ataaatgggt
agggttgcac 6540 caacaaaggg atgggatggg gggtagaaga tacgaggata
acggggctca atggcacaaa 6600 taagaacgaa tactgccatt aagactcgtg
atccagcgac tgacaccatt gcatcatcta 6660 agggcctcaa aactacctcg
gaactgctgc gctgatctgg acaccacaga ggttccgagc 6720 actttaggtt
gcaccaaatg tcccaccagg tgcaggcaga aaacgctgga acagcgtgta 6780
cagtttgtct taacaaaaag tgagggcgct gaggtcgagc agggtggtgt gacttgttat
6840 agcctttaga gctgcgaaag cgcgtatgga tttggctcat caggccagat
tgagggtctg 6900 tggacacatg tcatgttagt gtacttcaat cgccccctgg
atatagcccc gacaataggc 6960 cgtggcctca tttttttgcc ttccgcacat
ttccattgct cggtacccac accttgcttc 7020 tcctgcactt gccaacctta
atactggttt acattgacca acatcttaca agcggggggc 7080 ttgtctaggg
tatatataaa cagtggctct cccaatcggt tgccagtctc ttttttcctt 7140
tctttcccca cagattcgaa atctaaacta cacatcacac aatgcctgtt actgacgtcc
7200 ttaagcgaaa gtccggtgtc atcgtcggcg acgatgtccg agccgtgagt
atccacgaca 7260 agatcagtgt cgagacgacg cgttttgtgt aatgacacaa
tccgaaagtc gctagcaaca 7320 cacactctct acacaaacta acccagctct
ccatggagtc cattgctccc ttcctgccct 7380 ccaagatgcc tcaggacctg
ttcatggacc tcgccagcgc tatcggtgtc cgagctgctc 7440 cctacgtcga
tcccctggag gctgccctgg ttgcccaggc cgagaagtac attcccacca 7500
ttgtccatca cactcgaggc ttcctggttg ccgtggagtc tcccctggct cgagagctgc
7560 ctctgatgaa ccccttccac gtgctcctga tcgtgctcgc ctacctggtc
accgtgtttg 7620 tgggtatgca gatcatgaag aactttgaac gattcgaggt
caagaccttc tccctcctgc 7680 acaacttctg tctggtctcc atctccgcct
acatgtgcgg tggcatcctg tacgaggctt 7740 atcaggccaa ctatggactg
tttgagaacg ctgccgatca caccttcaag ggtctcccta 7800 tggctaagat
gatctggctc ttctacttct ccaagatcat ggagtttgtc gacaccatga 7860
tcatggtcct caagaagaac aaccgacaga tttcctttct gcacgtgtac caccactctt
7920 ccatcttcac catctggtgg ctggtcacct tcgttgctcc caacggtgaa
gcctacttct 7980 ctgctgccct gaactccttc atccacgtca tcatgtacgg
ctactacttt ctgtctgccc 8040 tgggcttcaa gcaggtgtcg ttcatcaagt
tctacatcac tcgatcccag atgacccagt 8100 tctgcatgat gtctgtccag
tcttcctggg acatgtacgc catgaaggtc cttggccgac 8160 ctggataccc
cttcttcatc accgctctgc tctggttcta catgtggacc atgctcggtc 8220
tcttctacaa cttttaccga aagaacgcca agctcgccaa gcaggccaag gctgacgctg
8280 ccaaggagaa ggccagaaag ctccagtaag cggccgcaag tgtggatggg
gaagtgagtg 8340 cccggttctg tgtgcacaat tggcaatcca agatggatgg
attcaacaca gggatatagc 8400 gagctacgtg gtggtgcgag gatatagcaa
cggatattta tgtttgacac ttgagaatgt 8460 acgatacaag cactgtccaa
gtacaatact aaacatactg tacatactca tactcgtacc 8520 cgggcaacgg
tttcacttga gtgcagtggc tagtgctctt actcgtacag tgtgcaatac 8580
tgcgtatcat agtctttgat gtatatcgta ttcattcatg ttagttgcgt acgaagtcgt
8640 caatgatgtc gatatgggtt ttgatcatgc acacataagg tccgacctta
tcggcaagct 8700 caatgagctc cttggtggtg gtaacatcca gagaagcaca
caggttggtt ttcttggctg 8760 ccacgagctt gagcactcga gcggcaaagg
cggacttgtg gacgttagct cgagcttcgt 8820 aggagggcat tttggtggtg
aagaggagac tgaaataaat ttagtctgca gaacttttta 8880 tcggaacctt
atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa 8940
cttatagata gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc
9000 tctgggcgtc gcctttgccg acaaaaatgt gatcatgatg aaagccagca
atgacgttgc 9060 agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc
tgtcagaccc acagcctcca 9120 acgaagaatg tatcgtcaaa gtgatccaag
cacactcata gttggagtcg tactccaaag 9180 gcggcaatga cgagtcagac
agatactcgt cgaccttttc cttgggaacc accaccgtca 9240 gcccttctga
ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg atagcagaat 9300
atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt
9360 atgtgccttc attgagaatc ggtgttgctg atttcaataa agtcttgaga
tcagtttggc 9420 gcgccagctg cattaatgaa tcggccaacg cgcggggaga
ggcggtttgc gtattgggcg 9480 ctcttccgct tcctcgctca ctgactcgct
gcgctcggtc gttcggctgc ggcgagcggt 9540 atcagctcac tcaaaggcgg
taatacggtt atccacagaa tcaggggata acgcaggaaa 9600 gaacatgtga
gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 9660
gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
9720 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt 9780 gcgctctcct gttccgaccc tgccgcttac cggatacctg
tccgcctttc tcccttcggg 9840 aagcgtggcg ctttctcata gctcacgctg
taggtatctc agttcggtgt aggtcgttcg 9900 ctccaagctg ggctgtgtgc
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 9960 taactatcgt
cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 10020
tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
10080 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
tgaagccagt 10140 taccttcgga aaaagagttg gtagctcttg atccggcaaa
caaaccaccg ctggtagcgg 10200 tggttttttt gtttgcaagc agcagattac
gcgcagaaaa aaaggatctc aagaagatcc 10260 tttgatcttt tctacggggt
ctgacgctca gtggaacgaa aactcacgtt aagggatttt 10320 ggtcatgaga
ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 10380
taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
10440 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt 10500 cgtgtagata actacgatac gggagggctt accatctggc
cccagtgctg caatgatacc 10560 gcgagaccca cgctcaccgg ctccagattt
atcagcaata aaccagccag ccggaagggc 10620 cgagcgcaga agtggtcctg
caactttatc cgcctccatc cagtctatta attgttgccg 10680 ggaagctaga
gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 10740
aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
10800 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc 10860 tccgatcgtt gtcagaagta agttggccgc agtgttatca
ctcatggtta tggcagcact 10920 gcataattct cttactgtca tgccatccgt
aagatgcttt tctgtgactg gtgagtactc 10980 aaccaagtca ttctgagaat
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 11040 acgggataat
accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 11100
ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
11160 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa 11220 aacaggaagg caaaatgccg caaaaaaggg aataagggcg
acacggaaat gttgaatact 11280 catactcttc ctttttcaat attattgaag
catttatcag ggttattgtc tcatgagcgg 11340 atacatattt gaatgtattt
agaaaaataa acaaataggg gttccgcgca catttccccg 11400 aaaagtgcca
cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 11460
tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
11520 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
aagaatagac 11580 cgagataggg ttgagtgttg ttccagtttg gaacaagagt
ccactattaa agaacgtgga 11640 ctccaacgtc aaagggcgaa aaaccgtcta
tcagggcgat ggcccactac gtgaaccatc 11700 accctaatca agttttttgg
ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 11760 gagcccccga
tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 11820
gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
11880 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
attcaggctg 11940 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
tattacgcca gctggcgaaa 12000 gggggatgtg ctgcaaggcg attaagttgg
gtaacgccag ggttttccca gtcacgacgt 12060 tgtaaaacga cggccagtga
attgtaatac gactcactat agggcgaatt gggcccgacg 12120 tcgcatgcag
tggtggtatt gtgactgggg atgtagttga gaataagtca tacacaagtc 12180
agctttcttc gagcctcata taagtataag tagttcaacg tattagcact gtacccagca
12240 tctccgtatc gagaaacaca acaacatgcc ccattggaca gatcatgcgg
atacacaggt 12300 tgtgcagtat catacatact cgatcagaca ggtcgtctga
ccatcataca agctgaacaa 12360 gcgctccata cttgcacgct ctctatatac
acagttaaat tacatatcca tagtctaacc 12420 tctaacagtt aatcttctgg
taagcctccc agccagcctt ctggtatcgc ttggcctcct 12480 caataggatc
tcggttctgg ccgtacagac ctcggccgac aattatgata tccgttccgg 12540
tagacatgac atcctcaaca gttcggtact gctgtccgag agcgtctccc ttgtcgtcaa
12600 gacccacccc gggggtcaga ataagccagt cctcagagtc gcccttaat 12649
<210> SEQ ID NO 25 <211> LENGTH: 819 <212> TYPE:
DNA <213> ORGANISM: Thraustochytrium aureum <220>
FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
synthetic elongase (codon-optimized) for Yarrowia lipolytica
<400> SEQUENCE: 25 atggccaact cctctgtctg ggacgacgtg
gtcggacgag tcgagaccgg tgtcgaccag 60 tggatggacg gagctaagcc
ctacgctctg accgacggtc tgcccatgat ggacgtctcc 120 accatgctcg
ccttcgaggt cggctacatg gccatgctgc tcttcggcat tcccatcatg 180
aagcagatgg agaagccctt cgagctgaag accatcaagc tgctccacaa cctgttcctc
240 ttcggactgt ccctctacat gtgcgtcgag accatccgac aggctatcct
gggtggctac 300 aaggtcttcg gcaacgacat ggagaagggc aacgagtccc
acgctcaggg catgtcccga 360 atcgtctacg tgttctacgt ctccaaggcc
tacgagttcc tggacaccgc tatcatgatc 420 ctgtgcaaga agttcaacca
ggtctccttc ctgcacgtgt accaccatgc caccatcttc 480 gccatctggt
gggctattgc caagtacgct cctggtggcg acgcctactt ctccgtcatc 540
ctcaactcct tcgtccacac cgtcatgtac gcctactact tcttttcctc tcagggcttc
600 ggcttcgtca agcccatcaa gccctacatc accactctgc agatgaccca
gttcatggct 660 atgctggtgc agtccctgta cgactacctc ttcccctgcg
actaccctca ggctctggtc 720 cagctgctcg gcgtgtacat gatcaccctg
ctcgctctgt tcggcaactt ctttgtccag 780 tcctacctga agaagcccaa
gaagtccaag accaactaa 819 <210> SEQ ID NO 26 <211>
LENGTH: 272 <212> TYPE: PRT <213> ORGANISM:
Thraustochytrium aureum <400> SEQUENCE: 26 Met Ala Asn Ser
Ser Val Trp Asp Asp Val Val Gly Arg Val Glu Thr 1 5 10 15 Gly Val
Asp Gln Trp Met Asp Gly Ala Lys Pro Tyr Ala Leu Thr Asp 20 25 30
Gly Leu Pro Met Met Asp Val Ser Thr Met Leu Ala Phe Glu Val Gly 35
40 45 Tyr Met Ala Met Leu Leu Phe Gly Ile Pro Ile Met Lys Gln Met
Glu 50 55 60 Lys Pro Phe Glu Leu Lys Thr Ile Lys Leu Leu His Asn
Leu Phe Leu 65 70 75 80 Phe Gly Leu Ser Leu Tyr Met Cys Val Glu Thr
Ile Arg Gln Ala Ile 85 90 95 Leu Gly Gly Tyr Lys Val Phe Gly Asn
Asp Met Glu Lys Gly Asn Glu 100 105 110 Ser His Ala Gln Gly Met Ser
Arg Ile Val Tyr Val Phe Tyr Val Ser 115 120 125 Lys Ala Tyr Glu Phe
Leu Asp Thr Ala Ile Met Ile Leu Cys Lys Lys 130 135 140 Phe Asn Gln
Val Ser Phe Leu His Val Tyr His His Ala Thr Ile Phe 145 150 155 160
Ala Ile Trp Trp Ala Ile Ala Lys Tyr Ala Pro Gly Gly Asp Ala Tyr 165
170 175 Phe Ser Val Ile Leu Asn Ser Phe Val His Thr Val Met Tyr Ala
Tyr 180 185 190 Tyr Phe Phe Ser Ser Gln Gly Phe Gly Phe Val Lys Pro
Ile Lys Pro 195 200 205 Tyr Ile Thr Thr Leu Gln Met Thr Gln Phe Met
Ala Met Leu Val Gln 210 215 220 Ser Leu Tyr Asp Tyr Leu Phe Pro Cys
Asp Tyr Pro Gln Ala Leu Val 225 230 235 240 Gln Leu Leu Gly Val Tyr
Met Ile Thr Leu Leu Ala Leu Phe Gly Asn 245 250 255 Phe Phe Val Gln
Ser Tyr Leu Lys Lys Pro Lys Lys Ser Lys Thr Asn 260 265 270
<210> SEQ ID NO 27 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-1A <400> SEQUENCE: 27
gghcaycayr tbtayacaaa 20 <210> SEQ ID NO 28 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-1B <400> SEQUENCE: 28 gghcaycayr tbtayaccaa 20 <210>
SEQ ID NO 29 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-1C <400> SEQUENCE: 29
gghcaycayr tbtayacgaa 20 <210> SEQ ID NO 30 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-1D <400> SEQUENCE: 30 gghcaycayr tbtayactaa 20 <210>
SEQ ID NO 31 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-5AR <400> SEQUENCE:
31 tgrtgvacaa yytgrwartt 20 <210> SEQ ID NO 32 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-5BR <400> SEQUENCE: 32 tgrtgvacta yytgrwartt 20 <210>
SEQ ID NO 33 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer 5-5CR <400> SEQUENCE:
33 tgrtgvacca yytgrwartt 20 <210> SEQ ID NO 34 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
5-5DR <400> SEQUENCE: 34 tgrtgvacga yytgrwartt 20 <210>
SEQ ID NO 35 <211> LENGTH: 27 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer ODMW480 <400> SEQUENCE:
35 ccgataccag tcaatttggt cagcctc 27 <210> SEQ ID NO 36
<211> LENGTH: 23 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: CDSIII 5' primer <400> SEQUENCE: 36 aagcagtggt
atcaacgcag agt 23 <210> SEQ ID NO 37 <211> LENGTH: 28
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer ODMW479
<400> SEQUENCE: 37 cttaaccttg cagttcttgt cggggatc 28
<210> SEQ ID NO 38 <211> LENGTH: 22 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer DNR CDS 5' <400>
SEQUENCE: 38 caacgcagag tggccattac gg 22 <210> SEQ ID NO 39
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer YL791 <400> SEQUENCE: 39 cacctcgcgc
attgtccgct taacgtc 27 <210> SEQ ID NO 40
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer YL792 <400> SEQUENCE: 40 tgcgaaggga
gaatcatacg tgtagac 27 <210> SEQ ID NO 41 <211> LENGTH:
26 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer ODMW469
<400> SEQUENCE: 41 cttcatcttc cggaccgcat tcttgc 26
<210> SEQ ID NO 42 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AUAP <400> SEQUENCE: 42
ggccacgcgt cgactagtac 20 <210> SEQ ID NO 43 <211>
LENGTH: 28 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
ODMW470 <400> SEQUENCE: 43 cctcactggg acctcattgc tgatcacc 28
<210> SEQ ID NO 44 <211> LENGTH: 32 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer YL794 <400> SEQUENCE:
44 tttccatggc tctcagtctt accacagaac ag 32 <210> SEQ ID NO 45
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer YL797 <400> SEQUENCE: 45 gtacatgtac
caagcttgga agcggtg 27 <210> SEQ ID NO 46 <211> LENGTH:
27 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer YL796
<400> SEQUENCE: 46 caccgcttcc aagcttggta catgtac 27
<210> SEQ ID NO 47 <211> LENGTH: 39 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer YL795 <400> SEQUENCE:
47 tttgcggccg cttaggaatc ctgtgcgtcc ttcacgcag 39 <210> SEQ ID
NO 48 <211> LENGTH: 4070 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pEgD5S <400> SEQUENCE: 48
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga
gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat
gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc
aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct
ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
420 tgcatctaga tccatggctc tctcccttac taccgagcag ctgctcgagc
gacccgacct 480 ggttgccatc gacggcattc tctacgatct ggaaggtctt
gccaaggtcc atcccggagg 540 cgacttgatc ctcgcttctg gtgcctccga
tgcttctcct ctgttctact ccatgcaccc 600 ttacgtcaag cccgagaact
cgaagctgct tcaacagttc gtgcgaggca agcacgaccg 660 aacctccaag
gacattgtct acacctacga ctctcccttt gcacaggacg tcaagcgaac 720
tatgcgagag gtcatgaaag gtcggaactg gtatgccaca cctggattct ggctgcgaac
780 cgttggcatc attgctgtca ccgccttttg cgagtggcac tgggctacta
ccggaatggt 840 gctgtggggt ctcttgactg gattcatgca catgcagatc
ggcctgtcca ttcagcacga 900 tgcctctcat ggtgccatca gcaaaaagcc
ctgggtcaac gctctctttg cctacggcat 960 cgacgtcatt ggatcgtcca
gatggatctg gctgcagtct cacatcatgc gacatcacac 1020 ctacaccaat
cagcatggtc tcgacctgga tgccgagtcc gcagaaccat tccttgtgtt 1080
ccacaactac cctgctgcca acactgctcg aaagtggttt caccgattcc aggcctggta
1140 catgtacctc gtgcttggag cctacggcgt ttcgctggtg tacaaccctc
tctacatctt 1200 ccgaatgcag cacaacgaca ccattcccga gtctgtcaca
gccatgcgag agaacggctt 1260 tctgcgacgg taccgaaccc ttgcattcgt
tatgcgagct ttcttcatct ttcgaaccgc 1320 cttcttgccc tggtatctca
ctggaacctc cctgctcatc accattcctc tggtgcccac 1380 tgctaccggt
gccttcctca ccttcttttt catcttgtct cacaacttcg atggctcgga 1440
gcgaatcccc gacaagaact gcaaggtcaa gagctccgag aaggacgttg aagccgatca
1500 gatcgactgg tacagagctc aggtggagac ctcttccacc tacggtggac
ccattgccat 1560 gttctttact ggcggtctca acttccagat cgagcatcac
ctctttcctc gaatgtcgtc 1620 ttggcactat cccttcgtgc agcaagctgt
ccgagagtgt tgcgaacgac acggagttcg 1680 gtacgtcttc taccctacca
ttgtgggcaa catcatttcc accctcaagt acatgcacaa 1740 agtcggtgtg
gttcactgtg tcaaggacgc tcaggattcc taagcggccg catcggatcc 1800
cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt
1860 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
gaagcataaa 1920 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
ttaattgcgt tgcgctcact 1980 gcccgctttc cagtcgggaa acctgtcgtg
ccagctgcat taatgaatcg gccaacgcgc 2040 ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 2100 ctcggtcgtt
cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2160
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
2220 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
ctgacgagca 2280 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
acaggactat aaagatacca 2340 ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt ccgaccctgc cgcttaccgg 2400 atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2460 gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2520
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2580 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
ggtatgtagg 2640 cggtgctaca gagttcttga agtggtggcc taactacggc
tacactagaa gaacagtatt 2700 tggtatctgc gctctgctga agccagttac
cttcggaaaa agagttggta gctcttgatc 2760 cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 2820 cagaaaaaaa
ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 2880
gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2940 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
agtaaacttg 3000 gtctgacagt taccaatgct taatcagtga ggcacctatc
tcagcgatct gtctatttcg 3060 ttcatccata gttgcctgac tccccgtcgt
gtagataact acgatacggg agggcttacc 3120 atctggcccc agtgctgcaa
tgataccgcg agacccacgc tcaccggctc cagatttatc 3180 agcaataaac
cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 3240
ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
3300 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
cgtttggtat 3360 ggcttcattc agctccggtt cccaacgatc aaggcgagtt
acatgatccc ccatgttgtg 3420 caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc agaagtaagt tggccgcagt 3480 gttatcactc atggttatgg
cagcactgca taattctctt actgtcatgc catccgtaag 3540 atgcttttct
gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 3600
accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
3660 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
tcttaccgct 3720 gttgagatcc agttcgatgt aacccactcg tgcacccaac
tgatcttcag catcttttac 3780 tttcaccagc gtttctgggt gagcaaaaac
aggaaggcaa aatgccgcaa aaaagggaat 3840 aagggcgaca cggaaatgtt
gaatactcat actcttcctt tttcaatatt attgaagcat 3900 ttatcagggt
tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 3960
aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
4020 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 4070
<210> SEQ ID NO 49 <211> LENGTH: 8438 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pDMW369 <400>
SEQUENCE: 49 ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt
ggcaatccaa 60
gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac
120 ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag
tacaatacta 180 aacatactgt acatactcat actcgtaccc gggcaacggt
ttcacttgag tgcagtggct 240 agtgctctta ctcgtacagt gtgcaatact
gcgtatcata gtctttgatg tatatcgtat 300 tcattcatgt tagttgcgta
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 360 gagtgagcta
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 420
tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
480 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
ctgcggcgag 540 cggtatcagc tcactcaaag gcggtaatac ggttatccac
agaatcaggg gataacgcag 600 gaaagaacat gtgagcaaaa ggccagcaaa
aggccaggaa ccgtaaaaag gccgcgttgc 660 tggcgttttt ccataggctc
cgcccccctg acgagcatca caaaaatcga cgctcaagtc 720 agaggtggcg
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 780
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
840 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
gtgtaggtcg 900 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
gcccgaccgc tgcgccttat 960 ccggtaacta tcgtcttgag tccaacccgg
taagacacga cttatcgcca ctggcagcag 1020 ccactggtaa caggattagc
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 1080 ggtggcctaa
ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 1140
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
1200 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag 1260 atcctttgat cttttctacg gggtctgacg ctcagtggaa
cgaaaactca cgttaaggga 1320 ttttggtcat gagattatca aaaaggatct
tcacctagat ccttttaaat taaaaatgaa 1380 gttttaaatc aatctaaagt
atatatgagt aaacttggtc tgacagttac caatgcttaa 1440 tcagtgaggc
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1500
ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
1560 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa 1620 gggccgagcg cagaagtggt cctgcaactt tatccgcctc
catccagtct attaattgtt 1680 gccgggaagc tagagtaagt agttcgccag
ttaatagttt gcgcaacgtt gttgccattg 1740 ctacaggcat cgtggtgtca
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1800 aacgatcaag
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1860
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
1920 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
actggtgagt 1980 actcaaccaa gtcattctga gaatagtgta tgcggcgacc
gagttgctct tgcccggcgt 2040 caatacggga taataccgcg ccacatagca
gaactttaaa agtgctcatc attggaaaac 2100 gttcttcggg gcgaaaactc
tcaaggatct taccgctgtt gagatccagt tcgatgtaac 2160 ccactcgtgc
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 2220
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
2280 tactcatact cttccttttt caatattatt gaagcattta tcagggttat
tgtctcatga 2340 gcggatacat atttgaatgt atttagaaaa ataaacaaat
aggggttccg cgcacatttc 2400 cccgaaaagt gccacctgac gcgccctgta
gcggcgcatt aagcgcggcg ggtgtggtgg 2460 ttacgcgcag cgtgaccgct
acacttgcca gcgccctagc gcccgctcct ttcgctttct 2520 tcccttcctt
tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 2580
ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
2640 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
acgttggagt 2700 ccacgttctt taatagtgga ctcttgttcc aaactggaac
aacactcaac cctatctcgg 2760 tctattcttt tgatttataa gggattttgc
cgatttcggc ctattggtta aaaaatgagc 2820 tgatttaaca aaaatttaac
gcgaatttta acaaaatatt aacgcttaca atttccattc 2880 gccattcagg
ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 2940
ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
3000 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
tatagggcga 3060 attgggtacc gggccccccc tcgaggtcga tggtgtcgat
aagcttgata tcgaattcat 3120 gtcacacaaa ccgatcttcg cctcaaggaa
acctaattct acatccgaga gactgccgag 3180 atccagtcta cactgattaa
ttttcgggcc aataatttaa aaaaatcgtg ttatataata 3240 ttatatgtat
tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata 3300
gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat
3360 tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa
aatatattgt 3420 atgaacttat ttttattact tagtattatt agacaactta
cttgctttat gaaaaacact 3480 tcctatttag gaaacaattt ataatggcag
ttcgttcatt taacaattta tgtagaataa 3540 atgttataaa tgcgtatggg
aaatcttaaa tatggatagc ataaatgata tctgcattgc 3600 ctaattcgaa
atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga 3660
aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag
3720 aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta
tgtactattc 3780 tcattgttca tacttctagt catttcatcc cacatattcc
ttggatttct ctccaatgaa 3840 tgacattcta tcttgcaaat tcaacaatta
taataagata taccaaagta gcggtatagt 3900 ggcaatcaaa aagcttctct
ggtgtgcttc tcgtatttat ttttattcta atgatccatt 3960 aaaggtatat
atttatttct tgttatataa tccttttgtt tattacatgg gctggataca 4020
taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact
4080 gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga
aaaaaaaaat 4140 cgtatttcca ggttagacgt tccgcagaat ctagaatgcg
gtatgcggta cattgttctt 4200 cgaacgtaaa agttgcgctc cctgagatat
tgtacatttt tgcttttaca agtacaagta 4260 catcgtacaa ctatgtacta
ctgttgatgc atccacaaca gtttgttttg tttttttttg 4320 tttttttttt
ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc 4380
cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt
4440 tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg
aaatcaacgg 4500 atgctcaatc gatttcgaca gtaattaatt aagtcataca
caagtcagct ttcttcgagc 4560 ctcatataag tataagtagt tcaacgtatt
agcactgtac ccagcatctc cgtatcgaga 4620 aacacaacaa catgccccat
tggacagatc atgcggatac acaggttgtg cagtatcata 4680 catactcgat
cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg 4740
cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc
4800 ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat
aggatctcgg 4860 ttctggccgt acagacctcg gccgacaatt atgatatccg
ttccggtaga catgacatcc 4920 tcaacagttc ggtactgctg tccgagagcg
tctcccttgt cgtcaagacc caccccgggg 4980 gtcagaataa gccagtcctc
agagtcgccc ttaggtcggt tctgggcaat gaagccaacc 5040 acaaactcgg
ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc 5100
agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg
5160 ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac
gtcctccttc 5220 ttctgttcag agacagtttc ctcggcacca gctcgcaggc
cagcaatgat tccggttccg 5280 ggtacaccgt gggcgttggt gatatcggac
cactcggcga ttcggtgaca ccggtactgg 5340 tgcttgacag tgttgccaat
atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc 5400 ttaagagcaa
gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg 5460
tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc
5520 tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
tgccacgagc 5580 ttgagcactc gagcggcaaa ggcggacttg tggacgttag
ctcgagcttc gtaggagggc 5640 attttggtgg tgaagaggag actgaaataa
atttagtctg cagaactttt tatcggaacc 5700 ttatctgggg cagtgaagta
tatgttatgg taatagttac gagttagttg aacttataga 5760 tagactggac
tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg 5820
tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata
5880 ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc
caacgaagaa 5940 tgtatcgtca aagtgatcca agcacactca tagttggagt
cgtactccaa aggcggcaat 6000 gacgagtcag acagatactc gtcgactcag
gcgacgacgg aattcctgca gcccatctgc 6060 agaattcagg agagaccggg
ttggcggcgt atttgtgtcc caaaaaacag ccccaattgc 6120 cccggagaag
acggccaggc cgcctagatg acaaattcaa caactcacag ctgactttct 6180
gccattgcca ctaggggggg gcctttttat atggccaagc caagctctcc acgtcggttg
6240 ggctgcaccc aacaataaat gggtagggtt gcaccaacaa agggatggga
tggggggtag 6300 aagatacgag gataacgggg ctcaatggca caaataagaa
cgaatactgc cattaagact 6360 cgtgatccag cgactgacac cattgcatca
tctaagggcc tcaaaactac ctcggaactg 6420 ctgcgctgat ctggacacca
cagaggttcc gagcacttta ggttgcacca aatgtcccac 6480 caggtgcagg
cagaaaacgc tggaacagcg tgtacagttt gtcttaacaa aaagtgaggg 6540
cgctgaggtc gagcagggtg gtgtgacttg ttatagcctt tagagctgcg aaagcgcgta
6600 tggatttggc tcatcaggcc agattgaggg tctgtggaca catgtcatgt
tagtgtactt 6660 caatcgcccc ctggatatag ccccgacaat aggccgtggc
ctcatttttt tgccttccgc 6720 acatttccat tgctcggtac ccacaccttg
cttctcctgc acttgccaac cttaatactg 6780 gtttacattg accaacatct
tacaagcggg gggcttgtct agggtatata taaacagtgg 6840 ctctcccaat
cggttgccag tctctttttt cctttctttc cccacagatt cgaaatctaa 6900
actacacatc acacaatgcc tgttactgac gtccttaagc gaaagtccgg tgtcatcgtc
6960 ggcgacgatg tccgagccgt gagtatccac gacaagatca gtgtcgagac
gacgcgtttt 7020 gtgtaatgac acaatccgaa agtcgctagc aacacacact
ctctacacaa actaacccag 7080 ctctccatgg ctctctccct tactaccgag
cagctgctcg agcgacccga cctggttgcc 7140 atcgacggca ttctctacga
tctggaaggt cttgccaagg tccatcccgg aggcgacttg 7200 atcctcgctt
ctggtgcctc cgatgcttct cctctgttct actccatgca cccttacgtc 7260
aagcccgaga actcgaagct gcttcaacag ttcgtgcgag gcaagcacga ccgaacctcc
7320 aaggacattg tctacaccta cgactctccc tttgcacagg acgtcaagcg
aactatgcga 7380 gaggtcatga aaggtcggaa ctggtatgcc acacctggat
tctggctgcg aaccgttggc 7440 atcattgctg tcaccgcctt ttgcgagtgg
cactgggcta ctaccggaat ggtgctgtgg 7500 ggtctcttga ctggattcat
gcacatgcag atcggcctgt ccattcagca cgatgcctct 7560
catggtgcca tcagcaaaaa gccctgggtc aacgctctct ttgcctacgg catcgacgtc
7620 attggatcgt ccagatggat ctggctgcag tctcacatca tgcgacatca
cacctacacc 7680 aatcagcatg gtctcgacct ggatgccgag tccgcagaac
cattccttgt gttccacaac 7740 taccctgctg ccaacactgc tcgaaagtgg
tttcaccgat tccaggcctg gtacatgtac 7800 ctcgtgcttg gagcctacgg
cgtttcgctg gtgtacaacc ctctctacat cttccgaatg 7860 cagcacaacg
acaccattcc cgagtctgtc acagccatgc gagagaacgg ctttctgcga 7920
cggtaccgaa cccttgcatt cgttatgcga gctttcttca tctttcgaac cgccttcttg
7980 ccctggtatc tcactggaac ctccctgctc atcaccattc ctctggtgcc
cactgctacc 8040 ggtgccttcc tcaccttctt tttcatcttg tctcacaact
tcgatggctc ggagcgaatc 8100 cccgacaaga actgcaaggt caagagctcc
gagaaggacg ttgaagccga tcagatcgac 8160 tggtacagag ctcaggtgga
gacctcttcc acctacggtg gacccattgc catgttcttt 8220 actggcggtc
tcaacttcca gatcgagcat cacctctttc ctcgaatgtc gtcttggcac 8280
tatcccttcg tgcagcaagc tgtccgagag tgttgcgaac gacacggagt tcggtacgtc
8340 ttctacccta ccattgtggg caacatcatt tccaccctca agtacatgca
caaagtcggt 8400 gtggttcact gtgtcaagga cgctcaggat tcctaagc 8438
<210> SEQ ID NO 50 <211> LENGTH: 19 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer T7 <400> SEQUENCE: 50
ggaaacagct atgaccatg 19 <210> SEQ ID NO 51 <211>
LENGTH: 459 <212> TYPE: PRT <213> ORGANISM: Rhizopus
stolonifer <220> FEATURE: GenBank Accession No. AAX22052
<221> NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION:
delta-6 desaturase <400> SEQUENCE: 51 Met Ser Thr Leu Asp Arg
Gln Ser Ile Phe Thr Ile Lys Glu Leu Glu 1 5 10 15 Ser Ile Ser Gln
Arg Ile His Asp Gly Asp Glu Glu Ala Met Lys Phe 20 25 30 Ile Ile
Ile Asp Lys Lys Val Tyr Asp Val Thr Glu Phe Ile Glu Asp 35 40 45
His Pro Gly Gly Ala Gln Val Leu Leu Thr His Val Gly Lys Asp Ala 50
55 60 Ser Asp Val Phe His Ala Met His Pro Glu Ser Ala Tyr Glu Val
Leu 65 70 75 80 Asn Asn Tyr Phe Val Gly Asp Val Gln Glu Thr Val Val
Thr Glu Lys 85 90 95 Ser Ser Ser Ala Gln Phe Ala Val Glu Met Arg
Gln Leu Arg Asp Gln 100 105 110 Leu Lys Lys Glu Gly Tyr Phe His Ser
Ser Lys Leu Phe Tyr Ala Tyr 115 120 125 Lys Val Leu Ser Thr Leu Ala
Ile Cys Ile Ala Gly Leu Ser Leu Leu 130 135 140 Tyr Ala Tyr Gly Arg
Thr Ser Thr Leu Ala Val Val Ala Ser Ala Ile 145 150 155 160 Thr Val
Gly Ile Phe Trp Gln Gln Cys Gly Trp Leu Ala His Asp Phe 165 170 175
Gly His His Gln Cys Phe Glu Asp Arg Thr Trp Asn Asp Val Leu Val 180
185 190 Val Phe Leu Gly Asn Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp
Lys 195 200 205 Asn Lys His Asn Thr His His Ala Ser Thr Asn Val His
Gly Gln Asp 210 215 220 Pro Asp Ile Asp Thr Ala Pro Val Leu Leu Trp
Asp Glu Tyr Ala Ser 225 230 235 240 Ala Ala Tyr Tyr Ala Ser Leu Asp
Gln Glu Pro Thr Met Val Ser Arg 245 250 255 Phe Leu Ala Glu Gln Val
Leu Pro His Gln Thr Arg Tyr Phe Phe Phe 260 265 270 Ile Leu Ala Phe
Ala Arg Leu Ser Trp Ala Leu Gln Ser Leu Ser Tyr 275 280 285 Ser Phe
Lys Lys Glu Ser Ile Asn Lys Ser Arg Gln Leu Asn Leu Phe 290 295 300
Glu Arg Val Cys Ile Val Gly His Trp Ala Leu Phe Ala Phe Cys Ile 305
310 315 320 Tyr Ser Trp Cys Ser Asn Val Tyr His Met Val Leu Phe Phe
Leu Val 325 330 335 Ser Gln Ala Thr Thr Gly Tyr Thr Leu Ala Leu Val
Phe Ala Leu Asn 340 345 350 His Asn Gly Met Pro Val Ile Thr Glu Glu
Lys Ala Glu Ser Met Glu 355 360 365 Phe Phe Glu Ile Gln Val Ile Thr
Gly Arg Asp Val Thr Leu Ser Pro 370 375 380 Leu Gly Asp Trp Phe Met
Gly Gly Leu Asn Tyr Gln Ile Glu His His 385 390 395 400 Val Phe Pro
Asn Met Pro Arg His Asn Leu Pro Thr Val Lys Pro Met 405 410 415 Val
Lys Ser Leu Cys Gln Lys Tyr Asp Ile Asn Tyr His Asp Thr Gly 420 425
430 Phe Leu Lys Gly Thr Leu Glu Val Leu Gln Thr Leu Asp Ile Thr Ser
435 440 445 Lys Leu Ser Leu Gln Leu Ser Lys Lys Ser Phe 450 455
<210> SEQ ID NO 52 <211> LENGTH: 695 <212> TYPE:
DNA <213> ORGANISM: Pavlova lutheri <400> SEQUENCE: 52
agggccaagg gtgccaacca ccttccacgt gagactacac accgtaggcc gatgggcaag
60 ggtggagacg gcggcgcgca ggcggtgagc gggaccgacg cgtctctcgc
tgaggtgagc 120 tccgtcgata gcaagagcgt gcacgtcgtg ctctacggca
agcgcgtgga tgtcacaaag 180 ttccagggct acgacgtggc ctggtggcgc
gcgcgccata acacgcacca cgtgtgcacc 240 aacgaggatg gttcggaccc
ggacatcaag acggcgcccc tgctcatcta cgtgcgagag 300 aacccgtcca
ttgccaagcg gctcaacttc ttccagcgct ggcagcagta ctactatgtg 360
ccgaccatgg ccatcctcga cctctactgg cgcctggagt ccatcgcgta cgtggctgtg
420 cgcctgccta agatgtggat gcaggccgcc gctcttgccg ctcactacgc
gctcctgtgc 480 tgggtcttcg cagcgcatct caacctcatc cctctcatga
tggttgcacg cggcttcgcg 540 acgggcatcg ttgtctttgc aacccactat
ggtgaggaca tcctcgaccg cgagcacgtc 600 gagggcatga cgctcgtcga
gcagaccgcc aagacctccc gtaacatcac gggcggctgg 660 ctagtgaacg
tgctcacggg cttcatctcc ctgca 695 <210> SEQ ID NO 53
<211> LENGTH: 1106 <212> TYPE: DNA <213>
ORGANISM: Pavlova lutheri <400> SEQUENCE: 53 agggccaagg
gtgccaacca ccttccacgt gagactacac accgtaggcc gatgggcaag 60
ggtggagacg gcggcgcgca ggcggtgagc gggaccgacg cgtctctcgc tgaggtgagc
120 tccgtcgata gcaagagcgt gcacgtcgtg ctctacggca agcgcgtgga
tgtcacaaag 180 ttccagggct acgacgtggc ctggtggcgc gcgcgccata
acacgcacca cgtgtgcacc 240 aacgaggatg gttcggaccc ggacatcaag
acggcgcccc tgctcatcta cgtgcgagag 300 aacccgtcca ttgccaagcg
gctcaacttc ttccagcgct ggcagcagta ctactatgtg 360 ccgaccatgg
ccatcctcga cctctactgg cgcctggagt ccatcgcgta cgtggctgtg 420
cgcctgccta agatgtggat gcaggccgcc gctcttgccg ctcactacgc gctcctgtgc
480 tgggtcttcg cagcgcatct caacctcatc cctctcatga tggttgcacg
cggcttcgcg 540 acgggcatcg ttgtctttgc aacccactat ggtgaggaca
tcctcgaccg cgagcacgtc 600 gagggcatga cgctcgtcga gcagaccgcc
aagacctccc gtaacatcac gggcggctgg 660 ctagtgaacg tgctcacggg
cttcatctcc ctgcagaccg agcatcacct cttccccatg 720 atgcccaccg
gcaacctaat gactatccag cccgaggtac gcgacttctt caagaagcat 780
ggcctcgagt accgcgaggg caacctcttc cagtgcgtgc accagaacat caaggctctc
840 gccttcgagc acctcctcca ctgagcgtca ccactcaagc gtcctaagtg
cacaggtact 900 gtcttctgac cgatggccgc gcggctccct cggctggcag
tggggccaac gagtggcctc 960 gcgggatcgg gcacgatcgg gcctccatga
aacttcagtg ttcagagaca agccgacaac 1020 ctccgcatcg tgagaaatct
tttaaagcag tatgttccat cacgccgctt ttgcagtcaa 1080 taacattacc
caaaaaaaaa aaaaaa 1106 <210> SEQ ID NO 54 <211> LENGTH:
287 <212> TYPE: PRT <213> ORGANISM: Pavlova lutheri
<400> SEQUENCE: 54 Arg Ala Lys Gly Ala Asn His Leu Pro Arg
Glu Thr Thr His Arg Arg 1 5 10 15 Pro Met Gly Lys Gly Gly Asp Gly
Gly Ala Gln Ala Val Ser Gly Thr 20 25 30 Asp Ala Ser Leu Ala Glu
Val Ser Ser Val Asp Ser Lys Ser Val His 35 40 45 Val Val Leu Tyr
Gly Lys Arg Val Asp Val Thr Lys Phe Gln Gly Tyr 50 55 60 Asp Val
Ala Trp Trp Arg Ala Arg His Asn Thr His His Val Cys Thr 65 70 75 80
Asn Glu Asp Gly Ser Asp Pro Asp Ile Lys Thr Ala Pro Leu Leu Ile 85
90 95 Tyr Val Arg Glu Asn Pro Ser Ile Ala Lys Arg Leu Asn Phe Phe
Gln 100 105 110 Arg Trp Gln Gln Tyr Tyr Tyr Val Pro Thr Met Ala Ile
Leu Asp Leu 115 120 125 Tyr Trp Arg Leu Glu Ser Ile Ala Tyr Val Ala
Val Arg Leu Pro Lys
130 135 140 Met Trp Met Gln Ala Ala Ala Leu Ala Ala His Tyr Ala Leu
Leu Cys 145 150 155 160 Trp Val Phe Ala Ala His Leu Asn Leu Ile Pro
Leu Met Met Val Ala 165 170 175 Arg Gly Phe Ala Thr Gly Ile Val Val
Phe Ala Thr His Tyr Gly Glu 180 185 190 Asp Ile Leu Asp Arg Glu His
Val Glu Gly Met Thr Leu Val Glu Gln 195 200 205 Thr Ala Lys Thr Ser
Arg Asn Ile Thr Gly Gly Trp Leu Val Asn Val 210 215 220 Leu Thr Gly
Phe Ile Ser Leu Gln Thr Glu His His Leu Phe Pro Met 225 230 235 240
Met Pro Thr Gly Asn Leu Met Thr Ile Gln Pro Glu Val Arg Asp Phe 245
250 255 Phe Lys Lys His Gly Leu Glu Tyr Arg Glu Gly Asn Leu Phe Gln
Cys 260 265 270 Val His Gln Asn Ile Lys Ala Leu Ala Phe Glu His Leu
Leu His 275 280 285 <210> SEQ ID NO 55 <211> LENGTH: 20
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer SeqE
<400> SEQUENCE: 55 cgacacactc caatctttcc 20 <210> SEQ
ID NO 56 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer SeqW <400> SEQUENCE: 56 ggtggctgga
gttagacatc 20 <210> SEQ ID NO 57 <211> LENGTH: 22
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer AP1
<400> SEQUENCE: 57 gtaatacgac tcactatagg gc 22 <210>
SEQ ID NO 58 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer GSP PvDES <400>
SEQUENCE: 58 ctgcgaagac ccagcacagg 20 <210> SEQ ID NO 59
<211> LENGTH: 22 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer M13-28Rev <400> SEQUENCE: 59 gtaatacgac
tcactatagg gc 22 <210> SEQ ID NO 60 <211> LENGTH: 21
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer PavDes
seq <400> SEQUENCE: 60 ttgtggcgct caatcatctc c 21 <210>
SEQ ID NO 61 <211> LENGTH: 1294 <212> TYPE: DNA
<213> ORGANISM: Pavlova lutheri <400> SEQUENCE: 61
ctttgcgagc gcggcgcaga cgattgcggc ccgtagtgat cgcggtgcgc attgctgtgt
60 ttctagtttt gctgacgccc ggcccgataa tgacaccttc tcccgtttga
aatactaata 120 agtaactata ttataatatt caaaggtggc gactatggat
ctccttttct aaagttcagc 180 ggaattggga atcggagaaa tttcgagata
tgtcataatc acgtgctcta tctcgaatga 240 accgcggccg gtgagcgatt
actcgggaag ccaattccta ttaacgagtc aggggggatc 300 tttgaggtga
gtcggccacg cagagagagc aaggaatcat cctcatccgc cgttctcgag 360
aaagagccaa gggtgccaac caccttccac gtgagactac acaccgtagg ccgatgggca
420 agggtggaga cggcggcgcg caggcggcga gcgggaccga cgcatctctc
gctgaggtga 480 gctccgtcga tagcaagagc gtgcgcgtcg tgctctacgg
caagcgcgtg gatgtcacaa 540 agttccagag ggcacacccg ggcgggagca
aggtgttccg catcttccag gagcgcgacg 600 cgacggagca gttcgagtct
taccactcgc ccaaggccat caagatgatg gagggcatgc 660 tcaagaagtc
ggaggatgcg cccgcttccg tgcccctgcc ctcgcggtcc accatgggca 720
cggagttcaa ggagatgatt gagcgccaca agagggctgg tctctacgac ccttgcccgt
780 tggacgagct gttcaagctc accatcgtcc ttgcgcccat cttcgtgggc
gcctatctcg 840 tgcggagcgg cgtctcgccc ctcgcgggcg cgctctccat
gggctttggc ttctacctcg 900 acggctggct tgctcacrac tacctgcatc
acgcagtctt caagggctcg gtcaacacgc 960 tcgtcaaggc gaacaacgcc
atgggatacg ccctcggctt cctccagggc tacgacgtgg 1020 cctggtggcg
cgcgcgccat aacacgcacc acgtgtgcac caacgaggat ggttcggacc 1080
cggacatcaa gacggcgccc ctgctcatct acgtgcgaga gaacccgtcc attgccaagc
1140 ggctcaactt cttccagcgc tggcagcagt actactatgt gccgaccatg
gccatcctcg 1200 acctctactg gcgcctggag tccatcgcgt acgtggctgt
gcgcctgcct aagatgtgga 1260 tgcaggccgc cgctcttgcc gctcactacg cgct
1294 <210> SEQ ID NO 62 <211> LENGTH: 1927 <212>
TYPE: DNA <213> ORGANISM: Pavlova lutheri <400>
SEQUENCE: 62 ctttgcgagc gcggcgcaga cgattgcggc ccgtagtgat cgcggtgcgc
attgctgtgt 60 ttctagtttt gctgacgccc ggcccgataa tgacaccttc
tcccgtttga aatactaata 120 agtaactata ttataatatt caaaggtggc
gactatggat ctccttttct aaagttcagc 180 ggaattggga atcggagaaa
tttcgagata tgtcataatc acgtgctcta tctcgaatga 240 accgcggccg
gtgagcgatt actcgggaag ccaattccta ttaacgagtc aggggggatc 300
tttgaggtga gtcggccacg cagagagagc aaggaatcat cctcatccgc cgttctcgag
360 aaagagccaa gggtgccaac caccttccac gtgagactac acaccgtagg
ccgatgggca 420 agggtggaga cggcggcgcg caggcggtga gcgggaccga
cgcgtctctc gctgaggtga 480 gctccgtcga tagcaagagc gtgcacgtcg
tgctctacgg caagcgcgtg gatgtcacaa 540 agttccagaa ggcacacccg
ggcgggagca aggtgttccg catcttccag gagcgcgacg 600 cgacggagca
gttcgagtct taccactcgc ccaaggccat caagatgatg gagggcatgc 660
tcaagaagtc ggaggatgcg cccgcttccg tgcccctgcc ctcgcggtcc accatgggca
720 cggagttcaa ggagatgatt gagcgccaca agagggctgg tctctacgac
ccttgcccgt 780 tggacgagct gttcaagctc accatcgtcc ttgcgcccat
cttcgtgggc gcctatctcg 840 tgcggagcgg cgtctcgccc ctcgcgggcg
cgctctccat gggctttggc ttctacctcg 900 acggctggct tgctcacgac
tacctgcatc acgcagtctt caagggctcg gtcaacacgc 960 tcgtcaaggc
gaacaacgcc atgggatacg ccctcggctt cctccagggc tacgacgtgg 1020
cctggtggcg cgcgcgccat aacacgcacc acgtgtgcac caacgaggat ggttcggacc
1080 cggacatcaa gacggcgccc ctgctcatct acgtgcgaga gaacccgtcc
attgccaagc 1140 ggctcaactt cttccagcgc tggcagcagt actactatgt
gccgaccatg gccatcctcg 1200 acctctactg gcgcctggag tccatcgcgt
acgtggctgt gcgcctgcct aagatgtgga 1260 tgcaggccgc cgctcttgcc
gctcactacg cgctcctgtg ctgggtcttc gcagcgcatc 1320 tcaacctcat
ccctctcatg atggttgcac gcggcttcgc gacgggcatc gttgtctttg 1380
caacccacta tggtgaggac atcctcgacc gcgagcacgt cgagggcatg acgctcgtcg
1440 agcagaccgc caagacctcc cgtaacatca cgggcggctg gctagtgaac
gtgctcacgg 1500 gcttcatctc cctgcagacc gagcatcacc tcttccccat
gatgcccacc ggcaacctaa 1560 tgactatcca gcccgaggta cgcgacttct
tcaagaagca tggcctcgag taccgcgagg 1620 gcaacctctt ccagtgcgtg
caccagaaca tcaaggctct cgccttcgag cacctcctcc 1680 actgagcgtc
accactcaag cgtcctaagt gcacaggtac tgtcttctga ccgatggccg 1740
cgcggctccc tcggctggca gtggggccaa cgagtggcct cgcgggatcg ggcacgatcg
1800 ggcctccatg aaacttcagt gttcagagac aagccgacaa cctccgcatc
gtgagaaatc 1860 ttttaaagca gtatgttcca tcacgccgct tttgcagtca
ataacattac ccaaaaaaaa 1920 aaaaaaa 1927 <210> SEQ ID NO 63
<211> LENGTH: 459 <212> TYPE: PRT <213> ORGANISM:
Rhizopus stolonifer <220> FEATURE: GenBank Accession No.
ABB96724 <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: delta-6 destaurase <400> SEQUENCE: 63 Met Ser
Thr Leu Asp Arg Gln Ser Ile Phe Thr Ile Lys Glu Leu Glu 1 5 10 15
Ser Ile Ser Gln Arg Ile His Asp Gly Asp Glu Glu Ala Met Lys Phe 20
25 30 Ile Ile Ile Asp Lys Lys Val Tyr Asp Val Thr Glu Phe Ile Glu
Asp 35 40 45 His Pro Gly Gly Ala Gln Val Leu Leu Thr His Val Gly
Lys Asp Ala 50 55 60 Ser Asp Val Phe His Ala Met His Pro Glu Ser
Ala Tyr Glu Val Leu
65 70 75 80 Asn Asn Tyr Phe Val Gly Asp Val Gln Glu Thr Val Val Thr
Glu Lys 85 90 95 Ser Ser Ser Ala Gln Phe Ala Val Glu Met Arg Gln
Leu Arg Asp Gln 100 105 110 Leu Lys Lys Glu Gly Tyr Phe His Ser Ser
Lys Leu Phe Tyr Ala Tyr 115 120 125 Lys Val Leu Ser Thr Leu Ala Ile
Cys Ile Ala Gly Leu Ser Pro Leu 130 135 140 Tyr Ala Tyr Gly Arg Thr
Ser Thr Leu Ala Val Val Ala Ser Ala Ile 145 150 155 160 Thr Val Gly
Ile Phe Trp Gln Gln Cys Gly Trp Leu Ala His Asp Phe 165 170 175 Gly
His His Gln Cys Phe Glu Asp Arg Thr Trp Asn Asp Val Leu Val 180 185
190 Val Phe Leu Gly Asn Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp Lys
195 200 205 Asn Lys His Asn Thr His His Ala Ser Thr Asn Val His Gly
Gln Asp 210 215 220 Pro Asp Ile Asp Thr Ala Pro Val Leu Leu Trp Asp
Glu Tyr Ala Ser 225 230 235 240 Ala Ala Tyr Tyr Ala Ser Leu Asp Gln
Glu Pro Thr Met Val Ser Arg 245 250 255 Phe Leu Ala Glu Gln Val Leu
Pro His Gln Thr Arg Tyr Phe Phe Phe 260 265 270 Ile Leu Ala Phe Ala
Arg Leu Ser Trp Ala Leu Gln Ser Leu Ser Tyr 275 280 285 Ser Phe Lys
Lys Glu Ser Ile Asn Lys Ser Arg Gln Leu Asn Leu Phe 290 295 300 Glu
Arg Val Cys Ile Val Gly His Trp Ala Leu Ser Ala Phe Cys Ile 305 310
315 320 Tyr Ser Trp Cys Ser Asn Val Tyr His Met Val Leu Phe Phe Leu
Val 325 330 335 Ser Gln Ala Thr Thr Gly Tyr Thr Leu Ala Leu Val Phe
Ala Leu Asn 340 345 350 His Asn Gly Met Pro Val Ile Thr Glu Glu Lys
Ala Glu Ser Met Glu 355 360 365 Phe Phe Glu Ile Gln Val Ile Thr Gly
Arg Asp Val Thr Leu Ser Pro 370 375 380 Leu Gly Asp Trp Phe Met Gly
Gly Leu Asn Tyr Gln Ile Glu His His 385 390 395 400 Val Phe Pro Asn
Met Pro Arg His Asn Leu Pro Thr Val Lys Pro Met 405 410 415 Val Lys
Ser Leu Cys Gln Lys Tyr Asp Ile Asn Tyr His Asp Thr Gly 420 425 430
Phe Leu Lys Gly Thr Leu Glu Val Leu Gln Thr Leu Asp Ile Thr Ser 435
440 445 Lys Leu Ser Leu Gln Leu Ser Lys Lys Ser Phe 450 455
<210> SEQ ID NO 64 <211> LENGTH: 427 <212> TYPE:
PRT <213> ORGANISM: Pavlova salina <300> PUBLICATION
INFORMATION: <302> TITLE: SYNTHESIS OF LONG-CHAIN
POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELLS <310> PATENT
DOCUMENT NUMBER: WO 2005/103253 <311> PATENT FILING DATE:
2005-04-22 <312> PUBLICATION DATE: 2005-11-03 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(427) <400> SEQUENCE: 64
Met Gly Arg Gly Gly Asp Ser Ser Gly Gln Ala His Pro Ala Ala Glu 1 5
10 15 Leu Ala Val Pro Ser Asp Arg Ala Glu Val Ser Asn Ala Asp Ser
Lys 20 25 30 Ala Leu His Ile Val Leu Tyr Gly Lys Arg Val Asp Val
Thr Lys Phe 35 40 45 Gln Arg Thr His Pro Gly Gly Ser Lys Val Phe
Arg Ile Phe Gln Asp 50 55 60 Arg Asp Ala Thr Glu Gln Phe Glu Ser
Tyr His Ser Lys Arg Ala Ile 65 70 75 80 Lys Met Met Glu Gly Met Leu
Lys Lys Ser Glu Asp Ala Pro Ala Asp 85 90 95 Thr Pro Leu Pro Ser
Gln Ser Pro Met Gly Lys Asp Phe Lys Ala Met 100 105 110 Ile Glu Arg
His Val Ala Ala Gly Tyr Tyr Asp Pro Cys Pro Leu Asp 115 120 125 Glu
Leu Phe Lys Leu Ser Leu Val Leu Leu Pro Thr Phe Ala Gly Met 130 135
140 Tyr Met Leu Lys Ala Gly Val Gly Ser Pro Leu Cys Gly Ala Leu Met
145 150 155 160 Val Ser Phe Gly Trp Tyr Leu Asp Gly Trp Leu Ala His
Asp Tyr Leu 165 170 175 His His Ser Val Phe Lys Gly Ser Val Ala Arg
Thr Val Gly Trp Asn 180 185 190 Asn Ala Ala Gly Tyr Phe Leu Gly Phe
Val Gln Gly Tyr Ala Val Glu 195 200 205 Trp Trp Arg Ala Arg His Asn
Thr His His Val Cys Thr Asn Glu Asp 210 215 220 Gly Ser Asp Pro Asp
Ile Lys Thr Ala Pro Leu Leu Ile Tyr Val Arg 225 230 235 240 Asn Lys
Pro Ser Ile Ala Lys Arg Leu Asn Ala Phe Gln Arg Tyr Gln 245 250 255
Gln Tyr Tyr Tyr Val Pro Val Met Ala Ile Leu Asp Leu Tyr Trp Arg 260
265 270 Leu Glu Ser Ile Ala Tyr Val Ala Met Arg Leu Pro Lys Met Leu
Pro 275 280 285 Gln Ala Leu Ala Leu Val Ala His Tyr Ala Ile Val Ala
Trp Val Phe 290 295 300 Ala Gly Asn Tyr His Leu Leu Pro Leu Val Thr
Val Leu Arg Gly Phe 305 310 315 320 Gly Thr Gly Ile Thr Val Phe Ala
Thr His Tyr Gly Glu Asp Ile Leu 325 330 335 Asp Ala Asp Gln Val Arg
His Met Thr Leu Val Glu Gln Thr Ala Leu 340 345 350 Thr Ser Arg Asn
Ile Ser Gly Gly Trp Leu Val Asn Val Leu Thr Gly 355 360 365 Phe Ile
Ser Leu Gln Thr Glu His His Leu Phe Pro Met Met Pro Thr 370 375 380
Gly Asn Leu Met Thr Ile Gln Pro Glu Val Arg Ala Phe Phe Lys Lys 385
390 395 400 His Gly Leu Glu Tyr Arg Glu Gly Asn Leu Ile Glu Cys Val
Arg Gln 405 410 415 Asn Ile Arg Ala Leu Ala Phe Glu His Leu Leu 420
425 <210> SEQ ID NO 65 <211> LENGTH: 37 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Primer AP <400>
SEQUENCE: 65 ggccacgcgt cgactagtac tttttttttt ttttttt 37
<210> SEQ ID NO 66 <211> LENGTH: 39 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Smart IV oligonucleotide primer
<400> SEQUENCE: 66 aagcagtggt atcaacgcag agtggccatt acggccggg
39 <210> SEQ ID NO 67 <211> LENGTH: 1338 <212>
TYPE: DNA <213> ORGANISM: Mortierella alpina <400>
SEQUENCE: 67 atgggaacgg accaaggaaa aaccttcacc tgggaagagc tggcggccca
taacaccaag 60 gacgacctac tcttggccat ccgcggcagg gtgtacgatg
tcacaaagtt cttgagccgc 120 catcctggtg gagtggacac tctcctgctc
ggagctggcc gagatgttac tccggtcttt 180 gagatgtatc acgcgtttgg
ggctgcagat gccattatga agaagtacta tgtcggtaca 240 ctggtctcga
atgagctgcc catcttcccg gagccaacgg tgttccacaa aaccatcaag 300
acgagagtcg agggctactt tacggatcgg aacattgatc ccaagaatag accagagatc
360 tggggacgat acgctcttat ctttggatcc ttgatcgctt cctactacgc
gcagctcttt 420 gtgcctttcg ttgtcgaacg cacatggctt caggtggtgt
ttgcaatcat catgggattt 480 gcgtgcgcac aagtcggact caaccctctt
catgatgcgt ctcacttttc agtgacccac 540 aaccccactg tctggaagat
tctgggagcc acgcacgact ttttcaacgg agcatcgtac 600 ctggtgtgga
tgtaccaaca tatgctcggc catcacccct acaccaacat tgctggagca 660
gatcccgacg tgtcgacgtc tgagcccgat gttcgtcgta tcaagcccaa ccaaaagtgg
720 tttgtcaacc acatcaacca gcacatgttt gttcctttcc tgtacggact
gctggcgttc 780 aaggtgcgca ttcaggacat caacattttg tactttgtca
agaccaatga cgctattcgt 840 gtcaatccca tctcgacatg gcacactgtg
atgttctggg gcggcaaggc tttctttgtc 900 tggtatcgcc tgattgttcc
cctgcagtat ctgcccctgg gcaaggtgct gctcttgttc 960 acggtcgcgg
acatggtgtc gtcttactgg ctggcgctga ccttccaggc gaaccacgtt 1020
gttgaggaag ttcagtggcc gttgcctgac gagaacggga tcatccaaaa ggactgggca
1080 gctatgcagg tcgagactac gcaggattac gcacacgatt cgcacctctg
gaccagcatc 1140 actggcagct tgaactacca ggctgtgcac catctgttcc
ccaacgtgtc gcagcaccat 1200 tatcccgata ttctggccat catcaagaac
acctgcagcg agtacaaggt tccatacctt 1260 gtcaaggata cgttttggca
agcatttgct tcacatttgg agcacttgcg tgttcttgga 1320 ctccgtccca
aggaagag 1338 <210> SEQ ID NO 68 <211> LENGTH: 446
<212> TYPE: PRT <213> ORGANISM: Mortierella alpina
<400> SEQUENCE: 68 Met Gly Thr Asp Gln Gly Lys Thr Phe Thr
Trp Glu Glu Leu Ala Ala 1 5 10 15 His Asn Thr Lys Asp Asp Leu Leu
Leu Ala Ile Arg Gly Arg Val Tyr 20 25 30 Asp Val Thr Lys Phe Leu
Ser Arg His Pro Gly Gly Val Asp Thr Leu 35 40 45 Leu Leu Gly Ala
Gly Arg Asp Val Thr Pro Val Phe Glu Met Tyr His 50 55 60 Ala Phe
Gly Ala Ala Asp Ala Ile Met Lys Lys Tyr Tyr Val Gly Thr 65 70 75 80
Leu Val Ser Asn Glu Leu Pro Ile Phe Pro Glu Pro Thr Val Phe His 85
90 95 Lys Thr Ile Lys Thr Arg Val Glu Gly Tyr Phe Thr Asp Arg Asn
Ile 100 105 110 Asp Pro Lys Asn Arg Pro Glu Ile Trp Gly Arg Tyr Ala
Leu Ile Phe 115 120 125 Gly Ser Leu Ile Ala Ser Tyr Tyr Ala Gln Leu
Phe Val Pro Phe Val 130 135 140 Val Glu Arg Thr Trp Leu Gln Val Val
Phe Ala Ile Ile Met Gly Phe 145 150 155 160 Ala Cys Ala Gln Val Gly
Leu Asn Pro Leu His Asp Ala Ser His Phe 165 170 175 Ser Val Thr His
Asn Pro Thr Val Trp Lys Ile Leu Gly Ala Thr His 180 185 190 Asp Phe
Phe Asn Gly Ala Ser Tyr Leu Val Trp Met Tyr Gln His Met 195 200 205
Leu Gly His His Pro Tyr Thr Asn Ile Ala Gly Ala Asp Pro Asp Val 210
215 220 Ser Thr Ser Glu Pro Asp Val Arg Arg Ile Lys Pro Asn Gln Lys
Trp 225 230 235 240 Phe Val Asn His Ile Asn Gln His Met Phe Val Pro
Phe Leu Tyr Gly 245 250 255 Leu Leu Ala Phe Lys Val Arg Ile Gln Asp
Ile Asn Ile Leu Tyr Phe 260 265 270 Val Lys Thr Asn Asp Ala Ile Arg
Val Asn Pro Ile Ser Thr Trp His 275 280 285 Thr Val Met Phe Trp Gly
Gly Lys Ala Phe Phe Val Trp Tyr Arg Leu 290 295 300 Ile Val Pro Leu
Gln Tyr Leu Pro Leu Gly Lys Val Leu Leu Leu Phe 305 310 315 320 Thr
Val Ala Asp Met Val Ser Ser Tyr Trp Leu Ala Leu Thr Phe Gln 325 330
335 Ala Asn His Val Val Glu Glu Val Gln Trp Pro Leu Pro Asp Glu Asn
340 345 350 Gly Ile Ile Gln Lys Asp Trp Ala Ala Met Gln Val Glu Thr
Thr Gln 355 360 365 Asp Tyr Ala His Asp Ser His Leu Trp Thr Ser Ile
Thr Gly Ser Leu 370 375 380 Asn Tyr Gln Ala Val His His Leu Phe Pro
Asn Val Ser Gln His His 385 390 395 400 Tyr Pro Asp Ile Leu Ala Ile
Ile Lys Asn Thr Cys Ser Glu Tyr Lys 405 410 415 Val Pro Tyr Leu Val
Lys Asp Thr Phe Trp Gln Ala Phe Ala Ser His 420 425 430 Leu Glu His
Leu Arg Val Leu Gly Leu Arg Pro Lys Glu Glu 435 440 445 <210>
SEQ ID NO 69 <211> LENGTH: 6473 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY5-22 <400> SEQUENCE:
69 ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg
gcgtaatcat 60 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
aattccacac aacgtacgag 120 ccggaagcat aaagtgtaaa gcctggggtg
cctaatgagt gagctaactc acattaattg 180 cgttgcgctc actgcccgct
ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 240 tcggccaacg
cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 300
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
360 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
gcaaaaggcc 420 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
gtttttccat aggctccgcc 480 cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac 540 tataaagata ccaggcgttt
ccccctggaa gctccctcgt gcgctctcct gttccgaccc 600 tgccgcttac
cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 660
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
720 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
cttgagtcca 780 acccggtaag acacgactta tcgccactgg cagcagccac
tggtaacagg attagcagag 840 cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta 900 gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg 960 gtagctcttg
atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 1020
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
1080 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
ttatcaaaaa 1140 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
taaatcaatc taaagtatat 1200 atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga 1260 tctgtctatt tcgttcatcc
atagttgcct gactccccgt cgtgtagata actacgatac 1320 gggagggctt
accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 1380
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
1440 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
gtaagtagtt 1500 cgccagttaa tagtttgcgc aacgttgttg ccattgctac
aggcatcgtg gtgtcacgct 1560 cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat 1620 cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 1680 agttggccgc
agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 1740
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
1800 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
accgcgccac 1860 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
ttcggggcga aaactctcaa 1920 ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt 1980 cagcatcttt tactttcacc
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 2040 caaaaaaggg
aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 2100
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
2160 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
cctgacgcgc 2220 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
gcgcagcgtg accgctacac 2280 ttgccagcgc cctagcgccc gctcctttcg
ctttcttccc ttcctttctc gccacgttcg 2340 ccggctttcc ccgtcaagct
ctaaatcggg ggctcccttt agggttccga tttagtgctt 2400 tacggcacct
cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 2460
cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
2520 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
ttataaggga 2580 ttttgccgat ttcggcctat tggttaaaaa atgagctgat
ttaacaaaaa tttaacgcga 2640 attttaacaa aatattaacg cttacaattt
ccattcgcca ttcaggctgc gcaactgttg 2700 ggaagggcga tcggtgcggg
cctcttcgct attacgccag ctggcgaaag ggggatgtgc 2760 tgcaaggcga
ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 2820
ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga
2880 ggtcgatggt gtcgataagc ttgatatcga attcatgtca cacaaaccga
tcttcgcctc 2940 aaggaaacct aattctacat ccgagagact gccgagatcc
agtctacact gattaatttt 3000 cgggccaata atttaaaaaa atcgtgttat
ataatattat atgtattata tatatacatc 3060 atgatgatac tgacagtcat
gtcccattgc taaatagaca gactccatct gccgcctcca 3120 actgatgttc
tcaatattta aggggtcatc tcgcattgtt taataataaa cagactccat 3180
ctaccgcctc caaatgatgt tctcaaaata tattgtatga acttattttt attacttagt
3240 attattagac aacttacttg ctttatgaaa aacacttcct atttaggaaa
caatttataa 3300 tggcagttcg ttcatttaac aatttatgta gaataaatgt
tataaatgcg tatgggaaat 3360 cttaaatatg gatagcataa atgatatctg
cattgcctaa ttcgaaatca acagcaacga 3420 aaaaaatccc ttgtacaaca
taaatagtca tcgagaaata tcaactatca aagaacagct 3480 attcacacgt
tactattgag attattattg gacgagaatc acacactcaa ctgtctttct 3540
ctcttctaga aatacaggta caagtatgta ctattctcat tgttcatact tctagtcatt
3600 tcatcccaca tattccttgg atttctctcc aatgaatgac attctatctt
gcaaattcaa 3660 caattataat aagatatacc aaagtagcgg tatagtggca
atcaaaaagc ttctctggtg 3720 tgcttctcgt atttattttt attctaatga
tccattaaag gtatatattt atttcttgtt 3780 atataatcct tttgtttatt
acatgggctg gatacataaa ggtattttga tttaattttt 3840 tgcttaaatt
caatcccccc tcgttcagtg tcaactgtaa tggtaggaaa ttaccatact 3900
tttgaagaag caaaaaaaat gaaagaaaaa aaaaatcgta tttccaggtt agacgttccg
3960 cagaatctag aatgcggtat gcggtacatt gttcttcgaa cgtaaaagtt
gcgctccctg 4020 agatattgta catttttgct tttacaagta caagtacatc
gtacaactat gtactactgt 4080 tgatgcatcc acaacagttt gttttgtttt
tttttgtttt ttttttttct aatgattcat 4140 taccgctatg tatacctact
tgtacttgta gtaagccggg ttattggcgt tcaattaatc 4200 atagacttat
gaatctgcac ggtgtgcgct gcgagttact tttagcttat gcatgctact 4260
tgggtgtaat attgggatct gttcggaaat caacggatgc tcaaccgatt tcgacagtaa
4320 ttaattaagt catacacaag tcagctttct tcgagcctca tataagtata
agtagttcaa 4380 cgtattagca ctgtacccag catctccgta tcgagaaaca
caacaacatg ccccattgga 4440 cagatcatgc ggatacacag gttgtgcagt
atcatacata ctcgatcaga caggtcgtct 4500 gaccatcata caagctgaac
aagcgctcca tacttgcacg ctctctatat acacagttaa 4560
attacatatc catagtctaa cctctaacag ttaatcttct ggtaagcctc ccagccagcc
4620 ttctggtatc gcttggcctc ctcaatagga tctcggttct ggccgtacag
acctcggccg 4680 acaattatga tatccgttcc ggtagacatg acatcctcaa
cagttcggta ctgctgtccg 4740 agagcgtctc ccttgtcgtc aagacccacc
ccgggggtca gaataagcca gtcctcagag 4800 tcgcccttag gtcggttctg
ggcaatgaag ccaaccacaa actcggggtc ggatcgggca 4860 agctcaatgg
tctgcttgga gtactcgcca gtggccagag agcccttgca agacagctcg 4920
gccagcatga gcagacctct ggccagcttc tcgttgggag aggggactag gaactccttg
4980 tactgggagt tctcgtagtc agagacgtcc tccttcttct gttcagagac
agtttcctcg 5040 gcaccagctc gcaggccagc aatgattccg gttccgggta
caccgtgggc gttggtgata 5100 tcggaccact cggcgattcg gtgacaccgg
tactggtgct tgacagtgtt gccaatatct 5160 gcgaactttc tgtcctcgaa
caggaagaaa ccgtgcttaa gagcaagttc cttgaggggg 5220 agcacagtgc
cggcgtaggt gaagtcgtca atgatgtcga tatgggtttt gatcatgcac 5280
acataaggtc cgaccttatc ggcaagctca atgagctcct tggtggtggt aacatccaga
5340 gaagcacaca ggttggtttt cttggctgcc acgagcttga gcactcgagc
ggcaaaggcg 5400 gacttgtgga cgttagctcg agcttcgtag gagggcattt
tggtggtgaa gaggagactg 5460 aaataaattt agtctgcaga actttttatc
ggaaccttat ctggggcagt gaagtatatg 5520 ttatggtaat agttacgagt
tagttgaact tatagataga ctggactata cggctatcgg 5580 tccaaattag
aaagaacgtc aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga 5640
tcatgatgaa agccagcaat gacgttgcag ctgatattgt tgtcggccaa ccgcgccgaa
5700 aacgcagctg tcagacccac agcctccaac gaagaatgta tcgtcaaagt
gatccaagca 5760 cactcatagt tggagtcgta ctccaaaggc ggcaatgacg
agtcagacag atactcgtcg 5820 actcaggcga cgacggaatt cctgcagccc
atctgcagaa ttcaggagag accgggttgg 5880 cggcgtattt gtgtcccaaa
aaacagcccc aattgcccca attgacccca aattgaccca 5940 gtagcgggcc
caaccccggc gagagccccc ttcaccccac atatcaaacc tcccccggtt 6000
cccacacttg ccgttaaggg cgtagggtac tgcagtctgg aatctacgct tgttcagact
6060 ttgtactagt ttctttgtct ggccatccgg gtaacccatg ccggacgcaa
aatagactac 6120 tgaaaatttt tttgctttgt ggttgggact ttagccaagg
gtataaaaga ccaccgtccc 6180 cgaattacct ttcctcttct tttctctctc
tccttgtcaa ctcacacccg aaatcgttaa 6240 gcatttcctt ctgagtataa
gaatcattca ccatggatcc actagttcta gagcggccgc 6300 caccgcggcc
cgagattccg gcctcttcgg ccgccaagcg acccgggtgg acgtctagag 6360
gtacctagca attaacagat agtttgccgg tgataattct cttaacctcc cacactcctt
6420 tgacataacg atttatgtaa cgaaactgaa atttgaccag atattgtgtc cgc
6473 <210> SEQ ID NO 70 <211> LENGTH: 6970 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pY5-22GPD
<400> SEQUENCE: 70 tcgacgcagt aggatgtcct gcacgggtct
ttttgtgggg tgtggagaaa ggggtgcttg 60 gagatggaag ccggtagaac
cgggctgctt gtgcttggag atggaagccg gtagaaccgg 120 gctgcttggg
gggatttggg gccgctgggc tccaaagagg ggtaggcatt tcgttggggt 180
tacgtaattg cggcatttgg gtcctgcgcg catgtcccat tggtcagaat tagtccggat
240 aggagactta tcagccaatc acagcgccgg atccacctgt aggttgggtt
gggtgggagc 300 acccctccac agagtagagt caaacagcag cagcaacatg
atagttgggg gtgtgcgtgt 360 taaaggaaaa aaaagaagct tgggttatat
tcccgctcta tttagaggtt gcgggataga 420 cgccgacgga gggcaatggc
gccatggaac cttgcggata tcgatacgcc gcggcggact 480 gcgtccgaac
cagctccagc agcgtttttt ccgggccatt gagccgactg cgaccccgcc 540
aacgtgtctt ggcccacgca ctcatgtcat gttggtgttg ggaggccact ttttaagtag
600 cacaaggcac ctagctcgca gcaaggtgtc cgaaccaaag aagcggctgc
agtggtgcaa 660 acggggcgga aacggcggga aaaagccacg ggggcacgaa
ttgaggcacg ccctcgaatt 720 tgagacgagt cacggcccca ttcgcccgcg
caatggctcg ccaacgcccg gtcttttgca 780 ccacatcagg ttaccccaag
ccaaaccttt gtgttaaaaa gcttaacata ttataccgaa 840 cgtaggtttg
ggcgggcttg ctccgtctgt ccaaggcaac atttatataa gggtctgcat 900
cgccggctca attgaatctt ttttcttctt ctcttctcta tattcattct tgaattaaac
960 acacatcaat ccgcggccgc caccgcggcc cgagattccg gcctcttcgg
ccgccaagcg 1020 acccgggtgg acgtctagag gtacctagca attaacagat
agtttgccgg tgataattct 1080 cttaacctcc cacactcctt tgacataacg
atttatgtaa cgaaactgaa atttgaccag 1140 atattgtgtc cgcggtggag
ctccagcttt tgttcccttt agtgagggtt aatttcgagc 1200 ttggcgtaat
catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 1260
cacaacgtac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
1320 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
gtcgtgccag 1380 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
tgcgtattgg gcgctcttcc 1440 gcttcctcgc tcactgactc gctgcgctcg
gtcgttcggc tgcggcgagc ggtatcagct 1500 cactcaaagg cggtaatacg
gttatccaca gaatcagggg ataacgcagg aaagaacatg 1560 tgagcaaaag
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 1620
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
1680 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
cgtgcgctct 1740 cctgttccga ccctgccgct taccggatac ctgtccgcct
ttctcccttc gggaagcgtg 1800 gcgctttctc atagctcacg ctgtaggtat
ctcagttcgg tgtaggtcgt tcgctccaag 1860 ctgggctgtg tgcacgaacc
ccccgttcag cccgaccgct gcgccttatc cggtaactat 1920 cgtcttgagt
ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 1980
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
2040 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
agttaccttc 2100 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
ccgctggtag cggtggtttt 2160 tttgtttgca agcagcagat tacgcgcaga
aaaaaaggat ctcaagaaga tcctttgatc 2220 ttttctacgg ggtctgacgc
tcagtggaac gaaaactcac gttaagggat tttggtcatg 2280 agattatcaa
aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 2340
atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
2400 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
cgtcgtgtag 2460 ataactacga tacgggaggg cttaccatct ggccccagtg
ctgcaatgat accgcgagac 2520 ccacgctcac cggctccaga tttatcagca
ataaaccagc cagccggaag ggccgagcgc 2580 agaagtggtc ctgcaacttt
atccgcctcc atccagtcta ttaattgttg ccgggaagct 2640 agagtaagta
gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 2700
gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
2760 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
tcctccgatc 2820 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
ttatggcagc actgcataat 2880 tctcttactg tcatgccatc cgtaagatgc
ttttctgtga ctggtgagta ctcaaccaag 2940 tcattctgag aatagtgtat
gcggcgaccg agttgctctt gcccggcgtc aatacgggat 3000 aataccgcgc
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 3060
cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
3120 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
aaaaacagga 3180 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
aatgttgaat actcatactc 3240 ttcctttttc aatattattg aagcatttat
cagggttatt gtctcatgag cggatacata 3300 tttgaatgta tttagaaaaa
taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 3360 ccacctgacg
cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 3420
gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
3480 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
tttagggttc 3540 cgatttagtg ctttacggca cctcgacccc aaaaaacttg
attagggtga tggttcacgt 3600 agtgggccat cgccctgata gacggttttt
cgccctttga cgttggagtc cacgttcttt 3660 aatagtggac tcttgttcca
aactggaaca acactcaacc ctatctcggt ctattctttt 3720 gatttataag
ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 3780
aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
3840 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
cagctggcga 3900 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
agggttttcc cagtcacgac 3960 gttgtaaaac gacggccagt gaattgtaat
acgactcact atagggcgaa ttgggtaccg 4020 ggccccccct cgaggtcgat
ggtgtcgata agcttgatat cgaattcatg tcacacaaac 4080 cgatcttcgc
ctcaaggaaa cctaattcta catccgagag actgccgaga tccagtctac 4140
actgattaat tttcgggcca ataatttaaa aaaatcgtgt tatataatat tatatgtatt
4200 atatatatac atcatgatga tactgacagt catgtcccat tgctaaatag
acagactcca 4260 tctgccgcct ccaactgatg ttctcaatat ttaaggggtc
atctcgcatt gtttaataat 4320 aaacagactc catctaccgc ctccaaatga
tgttctcaaa atatattgta tgaacttatt 4380 tttattactt agtattatta
gacaacttac ttgctttatg aaaaacactt cctatttagg 4440 aaacaattta
taatggcagt tcgttcattt aacaatttat gtagaataaa tgttataaat 4500
gcgtatggga aatcttaaat atggatagca taaatgatat ctgcattgcc taattcgaaa
4560 tcaacagcaa cgaaaaaaat cccttgtaca acataaatag tcatcgagaa
atatcaacta 4620 tcaaagaaca gctattcaca cgttactatt gagattatta
ttggacgaga atcacacact 4680 caactgtctt tctctcttct agaaatacag
gtacaagtat gtactattct cattgttcat 4740 acttctagtc atttcatccc
acatattcct tggatttctc tccaatgaat gacattctat 4800 cttgcaaatt
caacaattat aataagatat accaaagtag cggtatagtg gcaatcaaaa 4860
agcttctctg gtgtgcttct cgtatttatt tttattctaa tgatccatta aaggtatata
4920 tttatttctt gttatataat ccttttgttt attacatggg ctggatacat
aaaggtattt 4980 tgatttaatt ttttgcttaa attcaatccc ccctcgttca
gtgtcaactg taatggtagg 5040 aaattaccat acttttgaag aagcaaaaaa
aatgaaagaa aaaaaaaatc gtatttccag 5100 gttagacgtt ccgcagaatc
tagaatgcgg tatgcggtac attgttcttc gaacgtaaaa 5160 gttgcgctcc
ctgagatatt gtacattttt gcttttacaa gtacaagtac atcgtacaac 5220
tatgtactac tgttgatgca tccacaacag tttgttttgt ttttttttgt tttttttttt
5280 tctaatgatt cattaccgct atgtatacct acttgtactt gtagtaagcc
gggttattgg 5340
cgttcaatta atcatagact tatgaatctg cacggtgtgc gctgcgagtt acttttagct
5400 tatgcatgct acttgggtgt aatattggga tctgttcgga aatcaacgga
tgctcaaccg 5460 atttcgacag taattaatta agtcatacac aagtcagctt
tcttcgagcc tcatataagt 5520 ataagtagtt caacgtatta gcactgtacc
cagcatctcc gtatcgagaa acacaacaac 5580 atgccccatt ggacagatca
tgcggataca caggttgtgc agtatcatac atactcgatc 5640 agacaggtcg
tctgaccatc atacaagctg aacaagcgct ccatacttgc acgctctcta 5700
tatacacagt taaattacat atccatagtc taacctctaa cagttaatct tctggtaagc
5760 ctcccagcca gccttctggt atcgcttggc ctcctcaata ggatctcggt
tctggccgta 5820 cagacctcgg ccgacaatta tgatatccgt tccggtagac
atgacatcct caacagttcg 5880 gtactgctgt ccgagagcgt ctcccttgtc
gtcaagaccc accccggggg tcagaataag 5940 ccagtcctca gagtcgccct
taggtcggtt ctgggcaatg aagccaacca caaactcggg 6000 gtcggatcgg
gcaagctcaa tggtctgctt ggagtactcg ccagtggcca gagagccctt 6060
gcaagacagc tcggccagca tgagcagacc tctggccagc ttctcgttgg gagaggggac
6120 taggaactcc ttgtactggg agttctcgta gtcagagacg tcctccttct
tctgttcaga 6180 gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt
ccggttccgg gtacaccgtg 6240 ggcgttggtg atatcggacc actcggcgat
tcggtgacac cggtactggt gcttgacagt 6300 gttgccaata tctgcgaact
ttctgtcctc gaacaggaag aaaccgtgct taagagcaag 6360 ttccttgagg
gggagcacag tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt 6420
tttgatcatg cacacataag gtccgacctt atcggcaagc tcaatgagct ccttggtggt
6480 ggtaacatcc agagaagcac acaggttggt tttcttggct gccacgagct
tgagcactcg 6540 agcggcaaag gcggacttgt ggacgttagc tcgagcttcg
taggagggca ttttggtggt 6600 gaagaggaga ctgaaataaa tttagtctgc
agaacttttt atcggaacct tatctggggc 6660 agtgaagtat atgttatggt
aatagttacg agttagttga acttatagat agactggact 6720 atacggctat
cggtccaaat tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc 6780
gacaaaaatg tgatcatgat gaaagccagc aatgacgttg cagctgatat tgttgtcggc
6840 caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc aacgaagaat
gtatcgtcaa 6900 agtgatccaa gcacactcat agttggagtc gtactccaaa
ggcggcaatg acgagtcaga 6960 cagatactcg 6970 <210> SEQ ID NO 71
<211> LENGTH: 968 <212> TYPE: DNA <213> ORGANISM:
Yarrowia lipolytica <300> PUBLICATION INFORMATION:
<302> TITLE: GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND
PHOSPHOGLYCERATE MUTASE PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS
YEAST <310> PATENT DOCUMENT NUMBER: US-2005-0014270-A1
<311> PATENT FILING DATE: 2004-06-16 <312> PUBLICATION
DATE: 2005-01-20 <313> RELEVANT RESIDUES IN SEQ ID NO:
(1)..(968) <400> SEQUENCE: 71 tcgacgcagt aggatgtcct
gcacgggtct ttttgtgggg tgtggagaaa ggggtgcttg 60 gagatggaag
ccggtagaac cgggctgctt gtgcttggag atggaagccg gtagaaccgg 120
gctgcttggg gggatttggg gccgctgggc tccaaagagg ggtaggcatt tcgttggggt
180 tacgtaattg cggcatttgg gtcctgcgcg catgtcccat tggtcagaat
tagtccggat 240 aggagactta tcagccaatc acagcgccgg atccacctgt
aggttgggtt gggtgggagc 300 acccctccac agagtagagt caaacagcag
cagcaacatg atagttgggg gtgtgcgtgt 360 taaaggaaaa aaaagaagct
tgggttatat tcccgctcta tttagaggtt gcgggataga 420 cgccgacgga
gggcaatggc gccatggaac cttgcggata tcgatacgcc gcggcggact 480
gcgtccgaac cagctccagc agcgtttttt ccgggccatt gagccgactg cgaccccgcc
540 aacgtgtctt ggcccacgca ctcatgtcat gttggtgttg ggaggccact
ttttaagtag 600 cacaaggcac ctagctcgca gcaaggtgtc cgaaccaaag
aagcggctgc agtggtgcaa 660 acggggcgga aacggcggga aaaagccacg
ggggcacgaa ttgaggcacg ccctcgaatt 720 tgagacgagt cacggcccca
ttcgcccgcg caatggctcg ccaacgcccg gtcttttgca 780 ccacatcagg
ttaccccaag ccaaaccttt gtgttaaaaa gcttaacata ttataccgaa 840
cgtaggtttg ggcgggcttg ctccgtctgt ccaaggcaac atttatataa gggtctgcat
900 cgccggctca attgaatctt ttttcttctt ctcttctcta tattcattct
tgaattaaac 960 acacatca 968 <210> SEQ ID NO 72 <211>
LENGTH: 8630 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pYZDE2-S <400> SEQUENCE: 72 ggtggagctc cagcttttgt
tccctttagt gagggttaat ttcgagcttg gcgtaatcat 60 ggtcatagct
gtttcctgtg tgaaattgtt atccgctcac aattccacac aacgtacgag 120
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
180 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
cattaatgaa 240 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
ctcttccgct tcctcgctca 300 ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg 360 taatacggtt atccacagaa
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 420 agcaaaaggc
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 480
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
540 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc 600 tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg ctttctcata 660 gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc 720 acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca 780 acccggtaag
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 840
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
900 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
aaaagagttg 960 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
tggttttttt gtttgcaagc 1020 agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt 1080 ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 1140 ggatcttcac
ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 1200
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
1260 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
actacgatac 1320 gggagggctt accatctggc cccagtgctg caatgatacc
gcgagaccca cgctcaccgg 1380 ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg 1440 caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt 1500 cgccagttaa
tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 1560
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
1620 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta 1680 agttggccgc agtgttatca ctcatggtta tggcagcact
gcataattct cttactgtca 1740 tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat 1800 agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat acgggataat accgcgccac 1860 atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 1920
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
1980 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg 2040 caaaaaaggg aataagggcg acacggaaat gttgaatact
catactcttc ctttttcaat 2100 attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt 2160 agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgcgc 2220 cctgtagcgg
cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2280
ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2340 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
tttagtgctt 2400 tacggcacct cgaccccaaa aaacttgatt agggtgatgg
ttcacgtagt gggccatcgc 2460 cctgatagac ggtttttcgc cctttgacgt
tggagtccac gttctttaat agtggactct 2520 tgttccaaac tggaacaaca
ctcaacccta tctcggtcta ttcttttgat ttataaggga 2580 ttttgccgat
ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 2640
attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
2700 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
ggggatgtgc 2760 tgcaaggcga ttaagttggg taacgccagg gttttcccag
tcacgacgtt gtaaaacgac 2820 ggccagtgaa ttgtaatacg actcactata
gggcgaattg ggtaccgggc cccccctcga 2880 ggtcgatggt gtcgataagc
ttgatatcga attcatgtca cacaaaccga tcttcgcctc 2940 aaggaaacct
aattctacat ccgagagact gccgagatcc agtctacact gattaatttt 3000
cgggccaata atttaaaaaa atcgtgttat ataatattat atgtattata tatatacatc
3060 atgatgatac tgacagtcat gtcccattgc taaatagaca gactccatct
gccgcctcca 3120 actgatgttc tcaatattta aggggtcatc tcgcattgtt
taataataaa cagactccat 3180 ctaccgcctc caaatgatgt tctcaaaata
tattgtatga acttattttt attacttagt 3240 attattagac aacttacttg
ctttatgaaa aacacttcct atttaggaaa caatttataa 3300 tggcagttcg
ttcatttaac aatttatgta gaataaatgt tataaatgcg tatgggaaat 3360
cttaaatatg gatagcataa atgatatctg cattgcctaa ttcgaaatca acagcaacga
3420 aaaaaatccc ttgtacaaca taaatagtca tcgagaaata tcaactatca
aagaacagct 3480 attcacacgt tactattgag attattattg gacgagaatc
acacactcaa ctgtctttct 3540 ctcttctaga aatacaggta caagtatgta
ctattctcat tgttcatact tctagtcatt 3600 tcatcccaca tattccttgg
atttctctcc aatgaatgac attctatctt gcaaattcaa 3660 caattataat
aagatatacc aaagtagcgg tatagtggca atcaaaaagc ttctctggtg 3720
tgcttctcgt atttattttt attctaatga tccattaaag gtatatattt atttcttgtt
3780 atataatcct tttgtttatt acatgggctg gatacataaa ggtattttga
tttaattttt 3840 tgcttaaatt caatcccccc tcgttcagtg tcaactgtaa
tggtaggaaa ttaccatact 3900 tttgaagaag caaaaaaaat gaaagaaaaa
aaaaatcgta tttccaggtt agacgttccg 3960 cagaatctag aatgcggtat
gcggtacatt gttcttcgaa cgtaaaagtt gcgctccctg 4020
agatattgta catttttgct tttacaagta caagtacatc gtacaactat gtactactgt
4080 tgatgcatcc acaacagttt gttttgtttt tttttgtttt ttttttttct
aatgattcat 4140 taccgctatg tatacctact tgtacttgta gtaagccggg
ttattggcgt tcaattaatc 4200 atagacttat gaatctgcac ggtgtgcgct
gcgagttact tttagcttat gcatgctact 4260 tgggtgtaat attgggatct
gttcggaaat caacggatgc tcaaccgatt tcgacagtaa 4320 taatttgaat
cgaatcggag cctaaaatga acccgagtat atctcataaa attctcggtg 4380
agaggtctgt gactgtcagt acaaggtgcc ttcattatgc cctcaacctt accatacctc
4440 actgaatgta gtgtacctct aaaaatgaaa tacagtgcca aaagccaagg
cactgagctc 4500 gtctaacgga cttgatatac aaccaattaa aacaaatgaa
aagaaataca gttctttgta 4560 tcatttgtaa caattaccct gtacaaacta
aggtattgaa atcccacaat attcccaaag 4620 tccacccctt tccaaattgt
catgcctaca actcatatac caagcactaa cctaccaaac 4680 accactaaaa
ccccacaaaa tatatcttac cgaatataca gtaacaagct accaccacac 4740
tcgttgggtg cagtcgccag cttaaagata tctatccaca tcagccacaa ctcccttcct
4800 ttaataaacc gactacaccc ttggctattg aggttatgag tgaatatact
gtagacaaga 4860 cactttcaag aagactgttt ccaaaacgta ccactgtcct
ccactacaaa cacacccaat 4920 ctgcttcttc tagtcaaggt tgctacaccg
gtaaattata aatcatcatt tcattagcag 4980 ggcagggccc tttttataga
gtcttataca ctagcggacc ctgccggtag accaacccgc 5040 aggcgcgtca
gtttgctcct tccatcaatg cgtcgtagaa acgacttact ccttcttgag 5100
cagctccttg accttgttgg caacaagtct ccgacctcgg aggtggagga agagcctccg
5160 atatcggcgg tagtgatacc agcctcgacg gactccttga cggcagcctc
aacagcgtca 5220 ccggcgggct tcatgttaag agagaacttg agcatcatgg
cggcagacag aatggtggca 5280 atggggttga ccttctgctt gccgagatcg
ggggcagatc cgtgacaggg ctcgtacaga 5340 ccgaacgcct cgttggtgtc
gggcagagaa gccagagagg cggagggcag cagacccaga 5400 gaaccgggga
tgacggaggc ctcgtcggag atgatatcgc caaacatgtt ggtggtgatg 5460
atgataccat tcatcttgga gggctgcttg atgaggatca tggcggccga gtcgatcagc
5520 tggtggttga gctcgagctg ggggaattcg tccttgagga ctcgagtgac
agtctttcgc 5580 caaagtcgag aggaggccag cacgttggcc ttgtcaagag
accacacggg aagagggggg 5640 ttgtgctgaa gggccaggaa ggcggccatt
cgggcaattc gctcaacctc aggaacggag 5700 taggtctcgg tgtcggaagc
gacgccagat ccgtcatcct cctttcgctc tccaaagtag 5760 atacctccga
cgagctctcg gacaatgatg aagtcggtgc cctcaacgtt tcggatgggg 5820
gagagatcgg cgagcttggg cgacagcagc tggcagggtc gcaggttggc gtacaggttc
5880 aggtcctttc gcagcttgag gagaccctgc tcgggtcgca cgtcggttcg
tccgtcggga 5940 gtggtccata cggtgttggc agcgcctccg acagcaccga
gcataataga gtcagccttt 6000 cggcagatgt cgagagtagc gtcggtgatg
ggctcgccct ccttctcaat ggcagctcct 6060 ccaatgagtc ggtcctcaaa
cacaaactcg gtgccggagg cctcagcaac agacttgagc 6120 accttgacgg
cctcggcaat cacctcgggg ccacagaagt cgccgccgag aagaacaatc 6180
ttcttggagt cagtcttggt cttcttagtt tcgggttcca ttgtggatgt gtgtggttgt
6240 atgtgtgatg tggtgtgtgg agtgaaaatc tgtggctggc aaacgctctt
gtatatatac 6300 gcacttttgc ccgtgctatg tggaagacta aacctccgaa
gattgtgact caggtagtgc 6360 ggtatcggct agggacccaa accttgtcga
tgccgatagc gctatcgaac gtaccccagc 6420 cggccgggag tatgtcggag
gggacatacg agatcgtcaa gggtttgtgg ccaactggta 6480 aatttaaatg
atgtcgacgc agtaggatgt cctgcacggg tctttttgtg gggtgtggag 6540
aaaggggtgc ttggagatgg aagccggtag aaccgggctg cttgtgcttg gagatggaag
6600 ccggtagaac cgggctgctt ggggggattt ggggccgctg ggctccaaag
aggggtaggc 6660 atttcgttgg ggttacgtaa ttgcggcatt tgggtcctgc
gcgcatgtcc cattggtcag 6720 aattagtccg gataggagac ttatcagcca
atcacagcgc cggatccacc tgtaggttgg 6780 gttgggtggg agcacccctc
cacagagtag agtcaaacag cagcagcaac atgatagttg 6840 ggggtgtgcg
tgttaaagga aaaaaaagaa gcttgggtta tattcccgct ctatttagag 6900
gttgcgggat agacgccgac ggagggcaat ggcgccatgg aaccttgcgg atatcgatac
6960 gccgcggcgg actgcgtccg aaccagctcc agcagcgttt tttccgggcc
attgagccga 7020 ctgcgacccc gccaacgtgt cttggcccac gcactcatgt
catgttggtg ttgggaggcc 7080 actttttaag tagcacaagg cacctagctc
gcagcaaggt gtccgaacca aagaagcggc 7140 tgcagtggtg caaacggggc
ggaaacggcg ggaaaaagcc acgggggcac gaattgaggc 7200 acgccctcga
atttgagacg agtcacggcc ccattcgccc gcgcaatggc tcgccaacgc 7260
ccggtctttt gcaccacatc aggttacccc aagccaaacc tttgtgttaa aaagcttaac
7320 atattatacc gaacgtaggt ttgggcgggc ttgctccgtc tgtccaaggc
aacatttata 7380 taagggtctg catcgccggc tcaattgaat cttttttctt
cttctcttct ctatattcat 7440 tcttgaatta aacacacatc aatccatggc
aaacagcagc gtgtgggatg atgtggtggg 7500 ccgcgtggag accggcgtgg
accagtggat ggatggcgcc aagccgtacg cactcaccga 7560 tgggctcccg
atgatggacg tgtccaccat gctggcattc gaggtgggat acatggccat 7620
gctgctcttc ggcatcccga tcatgaagca gatggagaag ccttttgagc tcaagaccat
7680 caagctcttg cacaacttgt ttctcttcgg actttccttg tacatgtgcg
tggagaccat 7740 ccgccaggct atcctcggag gctacaaagt gtttggaaac
gacatggaga agggcaacga 7800 gtctcatgct cagggcatgt ctcgcatcgt
gtacgtgttc tacgtgtcca aggcatacga 7860 gttcttggat accgccatca
tgatcctttg caagaagttc aaccaggttt ccttcttgca 7920 tgtgtaccac
catgccactc atttttgcca tctggtgggc tatccgccaa gtacgctcca 7980
ggaggtgatg cgtacttttt cagtgatcct caactctttc gtgcacaccg tcatgtacgg
8040 catactactt cttctcctcc caagggttcg ggttcgtgaa gccaatcaag
ccgtacatca 8100 ccacccttca gatgacccag ttcatggcaa tgcttgtgca
gtccttgtac gactacctct 8160 tcccatgcga ctacccacag gctcttgtgc
agctccttgg agtgtacatg atcaccttgc 8220 ttgccctctt cggcaacttt
tttgtgcaga gctatcttaa aaagccaaaa aagagcaaga 8280 ccaactaaaa
ctgcctgcat gatatgccgc tcgccggcgt tcgaattgac tcagaaagcg 8340
agttaaggcg acacgcaaac tctatatttt ttcaaacgtg ttgccgtcac tcattcgcca
8400 tctgtttact acgtgtctgt tcaatgagca tgttcttgaa tctaaagaat
ctcgaatgtt 8460 ttttaaaaaa agaattcgat atcaagctta cgcgtcgacc
cgggtggacg tctagaggta 8520 cctagcaatt aacagatagt ttgccggtga
taattctctt aacctcccac actcctttga 8580 cataacgatt tatgtaacga
aactgaaatt tgaccagata ttgtgtccgc 8630 <210> SEQ ID NO 73
<211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer GPDsense <400> SEQUENCE: 73 atacgagatc
gtcaaggg 18 <210> SEQ ID NO 74 <211> LENGTH: 26
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer
GPDantisense <400> SEQUENCE: 74 gcggccgcgg attgatgtgt gtttaa
26 <210> SEQ ID NO 75 <211> LENGTH: 6339 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pKR136 <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(4078)..(4078) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 75 ggccgcgaca caagtgtgag agtactaaat
aaatgctttg gttgtacgaa atcattacac 60 taaataaaat aatcaaagct
tatatatgcc ttccgctaag gccgaatgca aagaaattgg 120 ttctttctcg
ttatcttttg ccacttttac tagtacgtat taattactac ttaatcatct 180
ttgtttacgg ctcattatat ccgtacgtcg agtcgacctg caggcatgca agcttggcgt
240 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
ccacacaaca 300 tacgagccgg aagcataaag tgtaaagcct ggggtgccta
atgagtgagc taactcacat 360 taattgcgtt gcgctcactg cccgctttcc
agtcgggaaa cctgtcgtgc cagctgcatt 420 aatgaatcgg ccaacgcgcg
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 480 cgctcactga
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 540
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
600 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
ttccataggc 660 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
tcagaggtgg cgaaacccga 720 caggactata aagataccag gcgtttcccc
ctggaagctc cctcgtgcgc tctcctgttc 780 cgaccctgcc gcttaccgga
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 840 ctcatagctc
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 900
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
960 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
aacaggatta 1020 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
gtggtggcct aactacggct 1080 acactagaag gacagtattt ggtatctgcg
ctctgctgaa gccagttacc ttcggaaaaa 1140 gagttggtag ctcttgatcc
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1200 gcaagcagca
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1260
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
1320 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
tcaatctaaa 1380 gtatatatga gtaaacttgg tctgacagtt accaatgctt
aatcagtgag gcacctatct 1440 cagcgatctg tctatttcgt tcatccatag
ttgcctgact ccccgtcgtg tagataacta 1500 cgatacggga gggcttacca
tctggcccca gtgctgcaat gataccgcga gacccacgct 1560 caccggctcc
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 1620
gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
1680 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
atcgtggtgt 1740 cacgctcgtc gtttggtatg gcttcattca gctccggttc
ccaacgatca aggcgagtta 1800
catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
1860 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
aattctctta 1920 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
gtactcaacc aagtcattct 1980 gagaatagtg tatgcggcga ccgagttgct
cttgcccggc gtcaatacgg gataataccg 2040 cgccacatag cagaacttta
aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2100 tctcaaggat
cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2160
gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
2220 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
ctcttccttt 2280 ttcaatatta ttgaagcatt tatcagggtt attgtctcat
gagcggatac atatttgaat 2340 gtatttagaa aaataaacaa ataggggttc
cgcgcacatt tccccgaaaa gtgccacctg 2400 acgtctaaga aaccattatt
atcatgacat taacctataa aaataggcgt atcacgaggc 2460 cctttcgtct
cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 2520
agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
2580 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
cagattgtac 2640 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
cgtaaggaga aaataccgca 2700 tcaggcgcca ttcgccattc aggctgcgca
actgttggga agggcgatcg gtgcgggcct 2760 cttcgctatt acgccagctg
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 2820 cgccagggtt
ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta 2880
cccggggatc ctctagacgt acgtcctcga agagaagggt taataacaca ttttttaaca
2940 tttttaacac aaattttagt tatttaaaaa tttattaaaa aatttaaaat
aagaagagga 3000 actctttaaa taaatctaac ttacaaaatt tatgattttt
aataagtttt caccaataaa 3060 aaatgtcata aaaatatgtt aaaaagtata
ttatcaatat tctctttatg ataaataaaa 3120 agaaaaaaaa aataaaagtt
aagtgaaaat gagattgaag tgactttagg tgtgtataaa 3180 tatatcaacc
ccgccaacaa tttatttaat ccaaatatat tgaagtatat tattccatag 3240
cctttattta tttatatatt tattatataa aagctttatt tgttctaggt tgttcatgaa
3300 atattttttt ggttttatct ccgttgtaag aaaatcatgt gctttgtgtc
gccactcact 3360 attgcagctt tttcatgcat tggtcagatt gacggttgat
tgtatttttg ttttttatgg 3420 ttttgtgtta tgacttaagt cttcatctct
ttatctcttc atcaggtttg atggttacct 3480 aatatggtcc atgggtacat
gcatggttaa attaggtggc caactttgtt gtgaacgata 3540 gaattttttt
tatattaagt aaactatttt tatattatga aataataata aaaaaaatat 3600
tttatcatta ttaacaaaat catattagtt aatttgttaa ctctataata aaagaaatac
3660 tgtaacattc acattacatg gtaacatctt tccacccttt catttgtttt
ttgtttgatg 3720 actttttttc ttgtttaaat ttatttccct tcttttaaat
ttggaataca ttatcatcat 3780 atataaacta aaatactaaa aacaggatta
cacaaatgat aaataataac acaaatattt 3840 ataaatctag ctgcaatata
tttaaactag ctatatcgat attgtaaaat aaaactagct 3900 gcattgatac
tgataaaaaa atatcatgtg ctttctggac tgatgatgca gtatactttt 3960
gacattgcct ttattttatt tttcagaaaa gctttcttag ttctgggttc ttcattattt
4020 gtttcccatc tccattgtga attgaatcat ttgcttcgtg tcacaaatac
aatttagnta 4080 ggtacatgca ttggtcagat tcacggttta ttatgtcatg
acttaagttc atggtagtac 4140 attacctgcc acgcatgcat tatattggtt
agatttgata ggcaaatttg gttgtcaaca 4200 atataaatat aaataatgtt
tttatattac gaaataacag tgatcaaaac aaacagtttt 4260 atctttatta
acaagatttt gtttttgttt gatgacgttt tttaatgttt acgctttccc 4320
ccttcttttg aatttagaac actttatcat cataaaatca aatactaaaa aaattacata
4380 tttcataaat aataacacaa atatttttaa aaaatctgaa ataataatga
acaatattac 4440 atattatcac gaaaattcat taataaaaat attatataaa
taaaatgtaa tagtagttat 4500 atgtaggaaa aaagtactgc acgcataata
tatacaaaaa gattaaaatg aactattata 4560 aataataaca ctaaattaat
ggtgaatcat atcaaaataa tgaaaaagta aataaaattt 4620 gtaattaact
tctatatgta ttacacacac aaataataaa taatagtaaa aaaaattatg 4680
ataaatattt accatctcat aagatattta aaataatgat aaaaatatag attatttttt
4740 atgcaactag ctagccaaaa agagaacacg ggtatatata aaaagagtac
ctttaaattc 4800 tactgtactt cctttattcc tgacgttttt atatcaagtg
gacatacgtg aagattttaa 4860 ttatcagtct aaatatttca ttagcactta
atacttttct gttttattcc tatcctataa 4920 gtagtcccga ttctcccaac
attgcttatt cacacaacta actaagaaag tcttccatag 4980 ccccccaagc
ggccgcatgg gaacggacca aggaaaaacc ttcacctggg aagagctggc 5040
ggcccataac accaaggacg acctactctt ggccatccgc ggcagggtgt acgatgtcac
5100 aaagttcttg agccgccatc ctggtggagt ggacactctc ctgctcggag
ctggccgaga 5160 tgttactccg gtctttgaga tgtatcacgc gtttggggct
gcagatgcca ttatgaagaa 5220 gtactatgtc ggtacactgg tctcgaatga
gctgcccatc ttcccggagc caacggtgtt 5280 ccacaaaacc atcaagacga
gagtcgaggg ctactttacg gatcggaaca ttgatcccaa 5340 gaatagacca
gagatctggg gacgatacgc tcttatcttt ggatccttga tcgcttccta 5400
ctacgcgcag ctctttgtgc ctttcgttgt cgaacgcaca tggcttcagg tggtgtttgc
5460 aatcatcatg ggatttgcgt gcgcacaagt cggactcaac cctcttcatg
atgcgtctca 5520 cttttcagtg acccacaacc ccactgtctg gaagattctg
ggagccacgc acgacttttt 5580 caacggagca tcgtacctgg tgtggatgta
ccaacatatg ctcggccatc acccctacac 5640 caacattgct ggagcagatc
ccgacgtgtc gacgtctgag cccgatgttc gtcgtatcaa 5700 gcccaaccaa
aagtggtttg tcaaccacat caaccagcac atgtttgttc ctttcctgta 5760
cggactgctg gcgttcaagg tgcgcattca ggacatcaac attttgtact ttgtcaagac
5820 caatgacgct attcgtgtca atcccatctc gacatggcac actgtgatgt
tctggggcgg 5880 caaggctttc tttgtctggt atcgcctgat tgttcccctg
cagtatctgc ccctgggcaa 5940 ggtgctgctc ttgttcacgg tcgcggacat
ggtgtcgtct tactggctgg cgctgacctt 6000 ccaggcgaac cacgttgttg
aggaagttca gtggccgttg cctgacgaga acgggatcat 6060 ccaaaaggac
tgggcagcta tgcaggtcga gactacgcag gattacgcac acgattcgca 6120
cctctggacc agcatcactg gcagcttgaa ctaccaggct gtgcaccatc tgttccccaa
6180 cgtgtcgcag caccattatc ccgatattct ggccatcatc aagaacacct
gcagcgagta 6240 caaggttcca taccttgtca aggatacgtt ttggcaagca
tttgcttcac atttggagca 6300 cttgcgtgtt cttggactcc gtcccaagga
agagtaggc 6339 <210> SEQ ID NO 76 <211> LENGTH: 8319
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid pY98
<400> SEQUENCE: 76 ggccgccacc gcggcccgag attccggcct
cttcggccgc caagcgaccc gggtggacgt 60 ctagaggtac ctagcaatta
acagatagtt tgccggtgat aattctctta acctcccaca 120 ctcctttgac
ataacgattt atgtaacgaa actgaaattt gaccagatat tgtgtccgcg 180
gtggagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
240 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
acgtacgagc 300 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
agctaactca cattaattgc 360 gttgcgctca ctgcccgctt tccagtcggg
aaacctgtcg tgccagctgc attaatgaat 420 cggccaacgc gcggggagag
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 480 tgactcgctg
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 540
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
600 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
ggctccgccc 660 ccctgacgag catcacaaaa atcgacgctc aagtcagagg
tggcgaaacc cgacaggact 720 ataaagatac caggcgtttc cccctggaag
ctccctcgtg cgctctcctg ttccgaccct 780 gccgcttacc ggatacctgt
ccgcctttct cccttcggga agcgtggcgc tttctcatag 840 ctcacgctgt
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 900
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
960 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
ttagcagagc 1020 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
cctaactacg gctacactag 1080 aaggacagta tttggtatct gcgctctgct
gaagccagtt accttcggaa aaagagttgg 1140 tagctcttga tccggcaaac
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1200 gcagattacg
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 1260
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
1320 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata 1380 tgagtaaact tggtctgaca gttaccaatg cttaatcagt
gaggcaccta tctcagcgat 1440 ctgtctattt cgttcatcca tagttgcctg
actccccgtc gtgtagataa ctacgatacg 1500 ggagggctta ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc 1560 tccagattta
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 1620
aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
1680 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
tgtcacgctc 1740 gtcgtttggt atggcttcat tcagctccgg ttcccaacga
tcaaggcgag ttacatgatc 1800 ccccatgttg tgcaaaaaag cggttagctc
cttcggtcct ccgatcgttg tcagaagtaa 1860 gttggccgca gtgttatcac
tcatggttat ggcagcactg cataattctc ttactgtcat 1920 gccatccgta
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 1980
gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
2040 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag 2100 gatcttaccg ctgttgagat ccagttcgat gtaacccact
cgtgcaccca actgatcttc 2160 agcatctttt actttcacca gcgtttctgg
gtgagcaaaa acaggaaggc aaaatgccgc 2220 aaaaaaggga ataagggcga
cacggaaatg ttgaatactc atactcttcc tttttcaata 2280 ttattgaagc
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 2340
gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
2400 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
ccgctacact 2460 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
tcctttctcg ccacgttcgc 2520 cggctttccc cgtcaagctc taaatcgggg
gctcccttta gggttccgat ttagtgcttt 2580 acggcacctc gaccccaaaa
aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 2640
ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
2700 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
tataagggat 2760 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
taacaaaaat ttaacgcgaa 2820 ttttaacaaa atattaacgc ttacaatttc
cattcgccat tcaggctgcg caactgttgg 2880 gaagggcgat cggtgcgggc
ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 2940 gcaaggcgat
taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 3000
gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
3060 gtcgatggtg tcgataagct tgatatcgaa ttcatgtcac acaaaccgat
cttcgcctca 3120 aggaaaccta attctacatc cgagagactg ccgagatcca
gtctacactg attaattttc 3180 gggccaataa tttaaaaaaa tcgtgttata
taatattata tgtattatat atatacatca 3240 tgatgatact gacagtcatg
tcccattgct aaatagacag actccatctg ccgcctccaa 3300 ctgatgttct
caatatttaa ggggtcatct cgcattgttt aataataaac agactccatc 3360
taccgcctcc aaatgatgtt ctcaaaatat attgtatgaa cttattttta ttacttagta
3420 ttattagaca acttacttgc tttatgaaaa acacttccta tttaggaaac
aatttataat 3480 ggcagttcgt tcatttaaca atttatgtag aataaatgtt
ataaatgcgt atgggaaatc 3540 ttaaatatgg atagcataaa tgatatctgc
attgcctaat tcgaaatcaa cagcaacgaa 3600 aaaaatccct tgtacaacat
aaatagtcat cgagaaatat caactatcaa agaacagcta 3660 ttcacacgtt
actattgaga ttattattgg acgagaatca cacactcaac tgtctttctc 3720
tcttctagaa atacaggtac aagtatgtac tattctcatt gttcatactt ctagtcattt
3780 catcccacat attccttgga tttctctcca atgaatgaca ttctatcttg
caaattcaac 3840 aattataata agatatacca aagtagcggt atagtggcaa
tcaaaaagct tctctggtgt 3900 gcttctcgta tttattttta ttctaatgat
ccattaaagg tatatattta tttcttgtta 3960 tataatcctt ttgtttatta
catgggctgg atacataaag gtattttgat ttaatttttt 4020 gcttaaattc
aatcccccct cgttcagtgt caactgtaat ggtaggaaat taccatactt 4080
ttgaagaagc aaaaaaaatg aaagaaaaaa aaaatcgtat ttccaggtta gacgttccgc
4140 agaatctaga atgcggtatg cggtacattg ttcttcgaac gtaaaagttg
cgctccctga 4200 gatattgtac atttttgctt ttacaagtac aagtacatcg
tacaactatg tactactgtt 4260 gatgcatcca caacagtttg ttttgttttt
ttttgttttt tttttttcta atgattcatt 4320 accgctatgt atacctactt
gtacttgtag taagccgggt tattggcgtt caattaatca 4380 tagacttatg
aatctgcacg gtgtgcgctg cgagttactt ttagcttatg catgctactt 4440
gggtgtaata ttgggatctg ttcggaaatc aacggatgct caaccgattt cgacagtaat
4500 taattaagtc atacacaagt cagctttctt cgagcctcat ataagtataa
gtagttcaac 4560 gtattagcac tgtacccagc atctccgtat cgagaaacac
aacaacatgc cccattggac 4620 agatcatgcg gatacacagg ttgtgcagta
tcatacatac tcgatcagac aggtcgtctg 4680 accatcatac aagctgaaca
agcgctccat acttgcacgc tctctatata cacagttaaa 4740 ttacatatcc
atagtctaac ctctaacagt taatcttctg gtaagcctcc cagccagcct 4800
tctggtatcg cttggcctcc tcaataggat ctcggttctg gccgtacaga cctcggccga
4860 caattatgat atccgttccg gtagacatga catcctcaac agttcggtac
tgctgtccga 4920 gagcgtctcc cttgtcgtca agacccaccc cgggggtcag
aataagccag tcctcagagt 4980 cgcccttagg tcggttctgg gcaatgaagc
caaccacaaa ctcggggtcg gatcgggcaa 5040 gctcaatggt ctgcttggag
tactcgccag tggccagaga gcccttgcaa gacagctcgg 5100 ccagcatgag
cagacctctg gccagcttct cgttgggaga ggggactagg aactccttgt 5160
actgggagtt ctcgtagtca gagacgtcct ccttcttctg ttcagagaca gtttcctcgg
5220 caccagctcg caggccagca atgattccgg ttccgggtac accgtgggcg
ttggtgatat 5280 cggaccactc ggcgattcgg tgacaccggt actggtgctt
gacagtgttg ccaatatctg 5340 cgaactttct gtcctcgaac aggaagaaac
cgtgcttaag agcaagttcc ttgaggggga 5400 gcacagtgcc ggcgtaggtg
aagtcgtcaa tgatgtcgat atgggttttg atcatgcaca 5460 cataaggtcc
gaccttatcg gcaagctcaa tgagctcctt ggtggtggta acatccagag 5520
aagcacacag gttggttttc ttggctgcca cgagcttgag cactcgagcg gcaaaggcgg
5580 acttgtggac gttagctcga gcttcgtagg agggcatttt ggtggtgaag
aggagactga 5640 aataaattta gtctgcagaa ctttttatcg gaaccttatc
tggggcagtg aagtatatgt 5700 tatggtaata gttacgagtt agttgaactt
atagatagac tggactatac ggctatcggt 5760 ccaaattaga aagaacgtca
atggctctct gggcgtcgcc tttgccgaca aaaatgtgat 5820 catgatgaaa
gccagcaatg acgttgcagc tgatattgtt gtcggccaac cgcgccgaaa 5880
acgcagctgt cagacccaca gcctccaacg aagaatgtat cgtcaaagtg atccaagcac
5940 actcatagtt ggagtcgtac tccaaaggcg gcaatgacga gtcagacaga
tactcgtcga 6000 cgcagtagga tgtcctgcac gggtcttttt gtggggtgtg
gagaaagggg tgcttggaga 6060 tggaagccgg tagaaccggg ctgcttgtgc
ttggagatgg aagccggtag aaccgggctg 6120 cttgggggga tttggggccg
ctgggctcca aagaggggta ggcatttcgt tggggttacg 6180 taattgcggc
atttgggtcc tgcgcgcatg tcccattggt cagaattagt ccggatagga 6240
gacttatcag ccaatcacag cgccggatcc acctgtaggt tgggttgggt gggagcaccc
6300 ctccacagag tagagtcaaa cagcagcagc aacatgatag ttgggggtgt
gcgtgttaaa 6360 ggaaaaaaaa gaagcttggg ttatattccc gctctattta
gaggttgcgg gatagacgcc 6420 gacggagggc aatggcgcca tggaaccttg
cggatatcga tacgccgcgg cggactgcgt 6480 ccgaaccagc tccagcagcg
ttttttccgg gccattgagc cgactgcgac cccgccaacg 6540 tgtcttggcc
cacgcactca tgtcatgttg gtgttgggag gccacttttt aagtagcaca 6600
aggcacctag ctcgcagcaa ggtgtccgaa ccaaagaagc ggctgcagtg gtgcaaacgg
6660 ggcggaaacg gcgggaaaaa gccacggggg cacgaattga ggcacgccct
cgaatttgag 6720 acgagtcacg gccccattcg cccgcgcaat ggctcgccaa
cgcccggtct tttgcaccac 6780 atcaggttac cccaagccaa acctttgtgt
taaaaagctt aacatattat accgaacgta 6840 ggtttgggcg ggcttgctcc
gtctgtccaa ggcaacattt atataagggt ctgcatcgcc 6900 ggctcaattg
aatctttttt cttcttctct tctctatatt cattcttgaa ttaaacacac 6960
atcaatccgc ggccgcatgg gaacggacca aggaaaaacc ttcacctggg aagagctggc
7020 ggcccataac accaaggacg acctactctt ggccatccgc ggcagggtgt
acgatgtcac 7080 aaagttcttg agccgccatc ctggtggagt ggacactctc
ctgctcggag ctggccgaga 7140 tgttactccg gtctttgaga tgtatcacgc
gtttggggct gcagatgcca ttatgaagaa 7200 gtactatgtc ggtacactgg
tctcgaatga gctgcccatc ttcccggagc caacggtgtt 7260 ccacaaaacc
atcaagacga gagtcgaggg ctactttacg gatcggaaca ttgatcccaa 7320
gaatagacca gagatctggg gacgatacgc tcttatcttt ggatccttga tcgcttccta
7380 ctacgcgcag ctctttgtgc ctttcgttgt cgaacgcaca tggcttcagg
tggtgtttgc 7440 aatcatcatg ggatttgcgt gcgcacaagt cggactcaac
cctcttcatg atgcgtctca 7500 cttttcagtg acccacaacc ccactgtctg
gaagattctg ggagccacgc acgacttttt 7560 caacggagca tcgtacctgg
tgtggatgta ccaacatatg ctcggccatc acccctacac 7620 caacattgct
ggagcagatc ccgacgtgtc gacgtctgag cccgatgttc gtcgtatcaa 7680
gcccaaccaa aagtggtttg tcaaccacat caaccagcac atgtttgttc ctttcctgta
7740 cggactgctg gcgttcaagg tgcgcattca ggacatcaac attttgtact
ttgtcaagac 7800 caatgacgct attcgtgtca atcccatctc gacatggcac
actgtgatgt tctggggcgg 7860 caaggctttc tttgtctggt atcgcctgat
tgttcccctg cagtatctgc ccctgggcaa 7920 ggtgctgctc ttgttcacgg
tcgcggacat ggtgtcgtct tactggctgg cgctgacctt 7980 ccaggcgaac
cacgttgttg aggaagttca gtggccgttg cctgacgaga acgggatcat 8040
ccaaaaggac tgggcagcta tgcaggtcga gactacgcag gattacgcac acgattcgca
8100 cctctggacc agcatcactg gcagcttgaa ctaccaggct gtgcaccatc
tgttccccaa 8160 cgtgtcgcag caccattatc ccgatattct ggccatcatc
aagaacacct gcagcgagta 8220 caaggttcca taccttgtca aggatacgtt
ttggcaagca tttgcttcac atttggagca 8280 cttgcgtgtt cttggactcc
gtcccaagga agagtaggc 8319 <210> SEQ ID NO 77 <211>
LENGTH: 774 <212> TYPE: DNA <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 77 atggaggtgg tgaatgaaat agtctcaatt
gggcaggaag ttttacccaa agttgattat 60 gcccaactct ggagtgatgc
cagtcactgt gaggtgcttt acttgtccat cgcatttgtc 120 atcttgaagt
tcactcttgg cccccttggt ccaaaaggtc agtctcgtat gaagtttgtt 180
ttcaccaatt acaaccttct catgtccatt tattcgttgg gatcattcct ctcaatggca
240 tatgccatgt acaccatcgg tgttatgtct gacaactgcg agaaggcttt
tgacaacaac 300 gtcttcagga tcaccacgca gttgttctat ttgagcaagt
tcctggagta tattgactcc 360 ttctatttgc cactgatggg caagcctctg
acctggttgc aattcttcca tcatttgggg 420 gcaccgatgg atatgtggct
gttctataat taccgaaatg aagctgtttg gatttttgtg 480 ctgttgaatg
gtttcatcca ctggatcatg tacggttatt attggaccag attgatcaag 540
ctgaagttcc ccatgccaaa atccctgatt acatcaatgc agatcattca attcaatgtt
600 ggtttctaca ttgtctggaa gtacaggaac attccctgtt atcgccaaga
tgggatgagg 660 atgtttggct ggttcttcaa ttacttttat gttggcacag
tcttgtgttt gttcttgaat 720 ttctatgtgc aaacgtatat cgtcaggaag
cacaagggag ccaaaaagat tcag 774 <210> SEQ ID NO 78 <211>
LENGTH: 1263 <212> TYPE: DNA <213> ORGANISM: Euglena
gracilis <400> SEQUENCE: 78 atgaagtcaa agcgccaagc gcttcccctt
acaattgatg gaacaacata tgatgtgtct 60 gcctgggtca atttccaccc
tggtggtgcg gaaattatag agaattacca aggaagggat 120 gccactgatg
ccttcatggt tatgcactct caagaagcct tcgacaagct caagcgcatg 180
cccaaaatca atcccagttc tgagttgcca ccccaggctg cagtgaatga agctcaagag
240 gatttccgga agctccgaga agagttgatc gcaactggca tgtttgatgc
ctcccccctc 300 tggtactcat acaaaatcag caccacactg ggccttggag
tgctgggtta tttcctgatg 360 gttcagtatc agatgtattt cattggggca
gtgttgcttg ggatgcacta tcaacagatg 420 ggctggcttt ctcatgacat
ttgccaccac cagactttca agaaccggaa ctggaacaac 480 ctcgtgggac
tggtatttgg caatggtctg caaggttttt ccgtgacatg gtggaaggac 540
agacacaatg cacatcattc ggcaaccaat gttcaagggc acgaccctga tattgacaac
600
ctccccctct tagcctggtc tgaggatgac gtcacacggg cgtcaccgat ttcccgcaag
660 ctcattcagt tccagcagta ctatttcttg gtcatctgta tcttgttgcg
gttcatttgg 720 tgtttccaga gcgtgttgac cgtgcgcagt ttgaaggaca
gagataacca attctatcgc 780 tctcagtata agaaggaggc cattggcctc
gccctgcact ggaccttgaa gaccctgttc 840 cacttattct ttatgcccag
catcctcaca tcgctgttgg tgtttttcgt ttcggagctg 900 gttggcggct
tcggcattgc gatcgtggtg ttcatgaacc actacccact ggagaagatc 960
ggggactcag tctgggatgg ccatggattc tcggttggcc agatccatga gaccatgaac
1020 attcggcgag ggattatcac agattggttt ttcggaggct tgaattacca
gattgagcac 1080 catttgtggc cgaccctccc tcgccacaac ctgacagcgg
ttagctacca ggtggaacag 1140 ctgtgccaga agcacaacct gccgtatcgg
aacccgctgc cccatgaagg gttggtcatc 1200 ctgctgcgct atctggcggt
gttcgcccgg atggcggaga agcaacccgc ggggaaggct 1260 cta 1263
<210> SEQ ID NO 79 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer oEugEL1-1 <400>
SEQUENCE: 79 agcggccgca ccatggaggt ggtgaatgaa 30 <210> SEQ ID
NO 80 <211> LENGTH: 30 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer oEugEL1-2 <400> SEQUENCE: 80
tgcggccgct cactgaatct ttttggctcc 30 <210> SEQ ID NO 81
<211> LENGTH: 4311 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR906 <400> SEQUENCE: 81
agcggccgca ccatggaggt ggtgaatgaa atagtctcaa ttgggcagga agttttaccc
60 aaagttgatt atgcccaact ctggagtgat gccagtcact gtgaggtgct
ttacttgtcc 120 atcgcatttg tcatcttgaa gttcactctt ggcccccttg
gtccaaaagg tcagtctcgt 180 atgaagtttg ttttcaccaa ttacaacctt
ctcatgtcca tttattcgtt gggatcattc 240 ctctcaatgg catatgccat
gtacaccatc ggtgttatgt ctgacaactg cgagaaggct 300 tttgacaaca
acgtcttcag gatcaccacg cagttgttct atttgagcaa gttcctggag 360
tatattgact ccttctattt gccactgatg ggcaagcctc tgacctggtt gcaattcttc
420 catcatttgg gggcaccgat ggatatgtgg ctgttctata attaccgaaa
tgaagctgtt 480 tggatttttg tgctgttgaa tggtttcatc cactggatca
tgtacggtta ttattggacc 540 agattgatca agctgaagtt ccccatgcca
aaatccctga ttacatcaat gcagatcatt 600 caattcaatg ttggtttcta
cattgtctgg aagtacagga acattccctg ttatcgccaa 660 gatgggatga
ggatgtttgg ctggttcttc aattactttt atgttggcac agtcttgtgt 720
ttgttcttga atttctatgt gcaaacgtat atcgtcagga agcacaaggg agccaaaaag
780 attcagtgag cggccgcacc tgaattccag cacactggcg gccgttacta
gtggatccga 840 gctcggtacc aagcttgatg catagcttga gtattctaac
gcgtcaccta aatagcttgg 900 cgtaatcatg gtcatagctg tttcctgtgt
gaaattgtta tccgctcaca attccacaca 960 acatacgagc cggaagcata
aagtgtaaag cctggggtgc ctaatgagtg agctaactca 1020 cattaattgc
gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 1080
attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
1140 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
tcagctcact 1200 caaaggcggt aatacggtta tccacagaat caggggataa
cgcaggaaag aacatgtgag 1260 caaaaggcca gcaaaagccc aggaaccgta
aaaaggccgc gttgctggcg tttttccata 1320 ggctccgccc ccctgacgag
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 1380 cgacaggact
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 1440
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
1500 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
tccaagctgg 1560 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
cttatccggt aactatcgtc 1620 ttgagtccaa cccggtaaga cacgacttat
cgccactggc agcagccact ggtaacagga 1680 ttagcagagc gaggtatgta
ggcggtgcta cagagttctt gaagtggtgg cctaactacg 1740 gctacactag
aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 1800
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
1860 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
ttgatctttt 1920 ctacggggtc tgacgctcag tggaacgaaa actcacgtta
agggattttg gtcatgagat 1980 tatcaaaaag gatcttcacc tagatccttt
taaattaaaa atgaagtttt agcacgtgtc 2040 agtcctgctc ctcggccacg
aagtgcacgc agttgccggc cgggtcgcgc agggcgaact 2100 cccgccccca
cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt 2160
tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
2220 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac
agggtcacgt 2280 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc
ccgggagaac ccgagccggt 2340 cggtccagaa ctcgaccgct ccggcgacgt
cgcgcgcggt gagcaccgga acggcactgg 2400 tcaacttggc catggtggcc
ctcctcacgt gctattattg aagcatttat cagggttatt 2460 gtctcatgag
cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 2520
gcacatttcc ccgaaaagtg ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag
2580 gagaaaatac cgcatcagga aattgtaagc gttaataatt cagaagaact
cgtcaagaag 2640 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
ccgtaaagca cgaggaagcg 2700 gtcagcccat tcgccgccaa gctcttcagc
aatatcacgg gtagccaacg ctatgtcctg 2760 atagcggtcc gccacaccca
gccggccaca gtcgatgaat ccagaaaagc ggccattttc 2820 caccatgata
ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg 2880
catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
2940 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
cgatgcgatg 3000 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
gtatgcagcc gccgcattgc 3060 atcagccatg atggatactt tctcggcagg
agcaaggtga gatgacagga gatcctgccc 3120 cggcacttcg cccaatagca
gccagtccct tcccgcttca gtgacaacgt cgagcacagc 3180 tgcgcaagga
acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc 3240
attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
3300 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
agccgaatag 3360 cctctccacc caagcggccg gagaacctgc gtgcaatcca
tcttgttcaa tcatgcgaaa 3420 cgatcctcat cctgtctctt gatcagagct
tgatcccctg cgccatcaga tccttggcgg 3480 cgagaaagcc atccagttta
ctttgcaggg cttcccaacc ttaccagagg gcgccccagc 3540 tggcaattcc
ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag 3600
cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
3660 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
acgtgaaaag 3720 gatctaggtg aagatccttt ttgataatct catgcctgac
atttatattc cccagaacat 3780 caggttaatg gcgtttttga tgtcattttc
gcggtggctg agatcagcca cttcttcccc 3840 gataacggag accggcacac
tggccatatc ggtggtcatc atgcgccagc tttcatcccc 3900 gatatgcacc
accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc 3960
cagggggatc accatccgtc gccccggcgt gtcaataata tcactctgta catccacaaa
4020 cagacgataa cggctctctc ttttataggt gtaaacctta aactgccgta
cgtataggct 4080 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
ctattacgcc agctggcgaa 4140 agggggatgt gctgcaaggc gattaagttg
ggtaacgcca gggttttccc agtcacgacg 4200 ttgtaaaacg acggccagtg
aattgtaata cgactcacta tagggcgaat tgggccctct 4260 agatgcatgc
tcgagcggcc gccagtgtga tggatatctg cagaattcag g 4311 <210> SEQ
ID NO 82 <211> LENGTH: 7085 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR72 <400> SEQUENCE: 82
gtacggatcc gtcgacggcg cgcccgatca tccggatata gttcctcctt tcagcaaaaa
60 acccctcaag acccgtttag aggccccaag gggttatgct agttattgct
cagcggtggc 120 agcagccaac tcagcttcct ttcgggcttt gttagcagcc
ggatcgatcc aagctgtacc 180 tcactattcc tttgccctcg gacgagtgct
ggggcgtcgg tttccactat cggcgagtac 240 ttctacacag ccatcggtcc
agacggccgc gcttctgcgg gcgatttgtg tacgcccgac 300 agtcccggct
ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc 360
gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
420 ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
gcgtctgctg 480 ctccatacaa gccaaccacg gcctccagaa gaagatgttg
gcgacctcgt attgggaatc 540 cccgaacatc gcctcgctcc agtcaatgac
cgctgttatg cggccattgt ccgtcaggac 600 attgttggag ccgaaatccg
cgtgcacgag gtgccggact tcggggcagt cctcggccca 660 aagcatcagc
tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt 720
ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
780 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
gatcggccgc 840 agcgatcgca tccatagcct ccgcgaccgg ctgcagaaca
gcgggcagtt cggtttcagg 900 caggtcttgc aacgtgacac cctgtgcacg
gcgggagatg caataggtca ggctctcgct 960 gaattcccca atgtcaagca
cttccggaat cgggagcgcg gccgatgcaa agtgccgata 1020 aacataacga
tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg 1080
ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
1140
ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
1200 cttttccatg ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
agggaaaccg 1260 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
tcgagatcga tccaattcca 1320 atcccacaaa aatctgagct taacagcaca
gttgctcctc tcagagcaga atcgggtatt 1380 caacaccctc atatcaacta
ctacgttgtg tataacggtc cacatgccgg tatatacgat 1440 gactggggtt
gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt 1500
gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca gcaaacagac
1560 aggttgaact tcatccccaa aggagaagct caactcaagc ccaagagctt
tgctaaggcc 1620 ctaacaagcc caccaaagca aaaagcccac tggctcacgc
taggaaccaa aaggcccagc 1680 agtgatccag ccccaaaaga gatctccttt
gccccggaga ttacaatgga cgatttcctc 1740 tatctttacg atctaggaag
gaagttcgaa ggtgaaggtg acgacactat gttcaccact 1800 gataatgaga
aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga 1860
gaggcctacg cagcaggtct catcaagacg atctacccga gtaacaatct ccaggagatc
1920 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
ttgcatcaag 1980 aacacagaga aagacatatt tctcaagatc agaagtacta
ttccagtatg gacgattcaa 2040 ggcttgcttc ataaaccaag gcaagtaata
gagattggag tctctaaaaa ggtagttcct 2100 actgaatcta aggccatgca
tggagtctaa gattcaaatc gaggatctaa cagaactcgc 2160 cgtgaagact
ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat 2220
cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt
2280 ctcagaagac caaagggcta ttgagacttt tcaacaaagg ataatttcgg
gaaacctcct 2340 cggattccat tgcccagcta tctgtcactt catcgaaagg
acagtagaaa aggaaggtgg 2400 ctcctacaaa tgccatcatt gcgataaagg
aaaggctatc attcaagatg cctctgccga 2460 cagtggtccc aaagatggac
ccccacccac gaggagcatc gtggaaaaag aagacgttcc 2520 aaccacgtct
tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc 2580
acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
2640 gaggacacgc tcgagctcat ttctctatta cttcagccat aacaaaagaa
ctcttttctc 2700 ttcttattaa accatgaaaa agcctgaact caccgcgacg
tctgtcgaga agtttctgat 2760 cgaaaagttc gacagcgtct ccgacctgat
gcagctctcg gagggcgaag aatctcgtgc 2820 tttcagcttc gatgtaggag
ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg 2880 tttctacaaa
gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga 2940
agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
3000 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
agccggtcgc 3060 ggaggccatg gatgcgatcg ctgcggccga tcttagccag
acgagcgggt tcggcccatt 3120 cggaccgcaa ggaatcggtc aatacactac
atggcgtgat ttcatatgcg cgattgctga 3180 tccccatgtg tatcactggc
aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 3240 ggctctcgat
gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca 3300
cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
3360 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
tcttctggag 3420 gccgtggttg gcttgtatgg agcagcagac gcgctacttc
gagcggaggc atccggagct 3480 tgcaggatcg ccgcggctcc gggcgtatat
gctccgcatt ggtcttgacc aactctatca 3540 gagcttggtt gacggcaatt
tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat 3600 cgtccgatcc
ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt 3660
ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
3720 tccgagggca aaggaatagt gaggtaccta aagaaggagt gcgtcgaagc
agatcgttca 3780 aacatttggc aataaagttt cttaagattg aatcctgttg
ccggtcttgc gatgattatc 3840 atataatttc tgttgaatta cgttaagcat
gtaataatta acatgtaatg catgacgtta 3900 tttatgagat gggtttttat
gattagagtc ccgcaattat acatttaata cgcgatagaa 3960 aacaaaatat
agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta 4020
gatcgatgtc gaatcgatca acctgcatta atgaatcggc caacgcgcgg ggagaggcgg
4080 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
cggtcgttcg 4140 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
cggttatcca cagaatcagg 4200 ggataacgca ggaaagaaca tgtgagcaaa
aggccagcaa aaggccagga accgtaaaaa 4260 ggccgcgttg ctggcgtttt
tccataggct ccgcccccct gacgagcatc acaaaaatcg 4320 acgctcaagt
cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 4380
tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
4440 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
atctcagttc 4500 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
ccccccgttc agcccgaccg 4560 ctgcgcctta tccggtaact atcgtcttga
gtccaacccg gtaagacacg acttatcgcc 4620 actggcagca gccactggta
acaggattag cagagcgagg tatgtaggcg gtgctacaga 4680 gttcttgaag
tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 4740
tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4800 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
gaaaaaaagg 4860 atctcaagaa gatcctttga tcttttctac ggggtctgac
gctcagtgga acgaaaactc 4920 acgttaaggg attttggtca tgacattaac
ctataaaaat aggcgtatca cgaggccctt 4980 tcgtctcgcg cgtttcggtg
atgacggtga aaacctctga cacatgcagc tcccggagac 5040 ggtcacagct
tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5100
gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
5160 agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca
taaccttatg 5220 tatcatacac atacgattta ggtgacacta tagaacggcg
cgccaagctt gttgaaacat 5280 ccctgaagtg tctcatttta ttttatttat
tctttgctga taaaaaaata aaataaaaga 5340 agctaagcac acggtcaacc
attgctctac tgctaaaagg gttatgtgta gtgttttact 5400 gcataaatta
tgcagcaaac aagacaactc aaattaaaaa atttcctttg cttgtttttt 5460
tgttgtctct gacttgactt tcttgtggaa gttggttgta taaggattgg gacaccattg
5520 tccttcttaa tttaatttta ttctttgctg ataaaaaaaa aaatttcata
tagtgttaaa 5580 taataatttg ttaaataacc aaaaagtcaa atatgtttac
tctcgtttaa ataattgaga 5640 ttcgtccagc aaggctaaac gattgtatag
atttatgaca atatttactt ttttatagat 5700 aaatgttata ttataataaa
tttatataca tatattatat gttatttatt attattttaa 5760 atccttcaat
attttatcaa accaactcat aatttttttt ttatctgtaa gaagcaataa 5820
aattaaatag acccacttta aggatgatcc aacctttata cagagtaaga gagttcaaat
5880 agtacccttt catatacata tcaactaaaa tattagaaat atcatggatc
aaaccttata 5940 aagacattaa ataagtggat aagtataata tataaatggg
tagtatataa tatataaatg 6000 gatacaaact tctctcttta taattgttat
gtctccttaa catcctaata taatacataa 6060 gtgggtaata tataatatat
aaatggagac aaacttcttc cattataatt gttatgtctt 6120 cttaacactt
atgtctcgtt cacaatgcta aggttagaat tgtttagaaa gtcttatagt 6180
acacatttgt ttttgtacta tttgaagcat tccataagcc gtcacgattc agatgattta
6240 taataataag aggaaattta tcatagaaca ataaggtgca tagatagagt
gttaatatat 6300 cataacatcc tttgtttatt catagaagaa gtgagatgga
gctcagttat tatactgtta 6360 catggtcgga tacaatattc catgctctcc
atgagctctt acacctacat gcattttagt 6420 tcatacttgc ggccgcagta
tatcttaaat tctttaatac ggtgtactag gatattgaac 6480 tggttcttga
tgatgaaaac ctgggccgag attgcagcta tttatagtca taggtcttgt 6540
taacatgcat ggacatttgg ccacggggtg gcatgcagtt tgacgggtgt tgaaataaac
6600 aaaaatgagg tggcggaaga gaatacgagt ttgaggttgg gttagaaaca
acaaatgtga 6660 gggctcatga tgggttgagt tggtgaatgt tttgggctgc
tcgattgaca cctttgtgag 6720 tacgtgttgt tgtgcatggc ttttggggtc
cagttttttt ttcttgacgc ggcgatcctg 6780 atcagctagt ggataagtga
tgtccactgt gtgtgattgc gtttttgttt gaattttatg 6840 aacttagaca
ttgctatgca aaggatactc tcattgtgtt ttgtcttctt ttgttccttg 6900
gctttttctt atgatccaag agactagtca gtgttgtggc attcgagact accaagatta
6960 attatgatgg gggaaggata agtaactgat tagtacggac tgttaccaaa
ttaattaata 7020 agcggcaaat gaagggcatg gatcaaaagc ttggatctcc
tgcaggatct ggccggccgg 7080 atctc 7085 <210> SEQ ID NO 83
<211> LENGTH: 2540 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKS102 <400> SEQUENCE: 83
cgatcatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc
60 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
cttcctttcg 120 ggctttgtta gcagccggat cgatccaagc tgtacctcac
tattcctttg ccctcggacg 180 agtgctgggg cgtcggtttc cactatcggc
gagtacttct acacagccat cggtccagac 240 ggccgcgctt ctgcgggcga
tttgtgtacg cccgacagtc ccggctccgg atcggacgat 300 tgcgtcgcat
cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg 360
atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
420 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
accacggcct 480 ccagaagaag atgttggcga cctcgtattg ggaatccccg
aacatcgcct cgctccagtc 540 aatgaccgct gttatgcggc cattgtccgt
caggacattg ttggagccga aatccgcgtg 600 cacgaggtgc cggacttcgg
ggcagtcctc ggcccaaagc atcagctcat cgagagcctg 660 cgcgacggac
gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc 720
agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
780 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
tagcctccgc 840 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg
tcttgcaacg tgacaccctg 900 tgcacggcgg gagatgcaat aggtcaggct
ctcgctgaat tccccaatgt caagcacttc 960 cggaatcggg agcgcggccg
atgcaaagtg ccgataaaca taacgatctt tgtagaaacc 1020 atcggcgcag
ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc 1080
acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
1140 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt tccatgggta
tatctccttc 1200 ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
ggtctcccta tagtgagtcg 1260
tattaatttc gcgggatcga gatctgatca acctgcatta atgaatcggc caacgcgcgg
1320 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
tcgctgcgct 1380 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
ggcggtaata cggttatcca 1440 cagaatcagg ggataacgca ggaaagaaca
tgtgagcaaa aggccagcaa aaggccagga 1500 accgtaaaaa ggccgcgttg
ctggcgtttt tccataggct ccgcccccct gacgagcatc 1560 acaaaaatcg
acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 1620
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
1680 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
cgctgtaggt 1740 atctcagttc ggtgtaggtc gttcgctcca agctgggctg
tgtgcacgaa ccccccgttc 1800 agcccgaccg ctgcgcctta tccggtaact
atcgtcttga gtccaacccg gtaagacacg 1860 acttatcgcc actggcagca
gccactggta acaggattag cagagcgagg tatgtaggcg 1920 gtgctacaga
gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 1980
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
2040 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
attacgcgca 2100 gaaaaaaagg atctcaagaa gatcctttga tcttttctac
ggggtctgac gctcagtgga 2160 acgaaaactc acgttaaggg attttggtca
tgacattaac ctataaaaat aggcgtatca 2220 cgaggccctt tcgtctcgcg
cgtttcggtg atgacggtga aaacctctga cacatgcagc 2280 tcccggagac
ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 2340
gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
2400 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac
aattaataca 2460 taaccttatg tatcatacac atacgattta ggtgacacta
tagaacggcg cgccaagctt 2520 ggatccgtcg acggcgcgcc 2540 <210>
SEQ ID NO 84 <211> LENGTH: 4359 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR197 <400> SEQUENCE:
84 cgcgcccgat catccggata tagttcctcc tttcagcaaa aaacccctca
agacccgttt 60 agaggcccca aggggttatg ctagttattg ctcagcggtg
gcagcagcca actcagcttc 120 ctttcgggct ttgttagcag ccggatcgat
ccaagctgta cctcactatt cctttgccct 180 cggacgagtg ctggggcgtc
ggtttccact atcggcgagt acttctacac agccatcggt 240 ccagacggcc
gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg 300
gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
360 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
gcggcgatcc 420 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
tgctccatac aagccaacca 480 cggcctccag aagaagatgt tggcgacctc
gtattgggaa tccccgaaca tcgcctcgct 540 ccagtcaatg accgctgtta
tgcggccatt gtccgtcagg acattgttgg agccgaaatc 600 cgcgtgcacg
aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag 660
agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
720 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
ttccttgcgg 780 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
gcagcgatcg catccatagc 840 ctccgcgacc ggctgcagaa cagcgggcag
ttcggtttca ggcaggtctt gcaacgtgac 900 accctgtgca cggcgggaga
tgcaataggt caggctctcg ctgaattccc caatgtcaag 960 cacttccgga
atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta 1020
gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
1080 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
tgtcgaactt 1140 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
ggcttttcca tgggtatatc 1200 tccttcttaa agttaaacaa aattatttct
agagggaaac cgttgtggtc tccctatagt 1260 gagtcgtatt aatttcgcgg
gatcgagatc tgatcaacct gcattaatga atcggccaac 1320 gcgcggggag
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 1380
tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
1440 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
cagcaaaagg 1500 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
taggctccgc ccccctgacg 1560 agcatcacaa aaatcgacgc tcaagtcaga
ggtggcgaaa cccgacagga ctataaagat 1620 accaggcgtt tccccctgga
agctccctcg tgcgctctcc tgttccgacc ctgccgctta 1680 ccggatacct
gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct 1740
gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
1800 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
aacccggtaa 1860 gacacgactt atcgccactg gcagcagcca ctggtaacag
gattagcaga gcgaggtatg 1920 taggcggtgc tacagagttc ttgaagtggt
ggcctaacta cggctacact agaaggacag 1980 tatttggtat ctgcgctctg
ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 2040 gatccggcaa
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2100
cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
2160 agtggaacga aaactcacgt taagggattt tggtcatgac attaacctat
aaaaataggc 2220 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
cggtgaaaac ctctgacaca 2280 tgcagctccc ggagacggtc acagcttgtc
tgtaagcgga tgccgggagc agacaagccc 2340 gtcagggcgc gtcagcgggt
gttggcgggt gtcggggctg gcttaactat gcggcatcag 2400 agcagattgt
actgagagtg caccatatgg acatattgtc gttagaacgc ggctacaatt 2460
aatacataac cttatgtatc atacacatac gatttaggtg acactataga acggcgcgcc
2520 aagcttgttg aaacatccct gaagtgtctc attttatttt atttattctt
tgctgataaa 2580 aaaataaaat aaaagaagct aagcacacgg tcaaccattg
ctctactgct aaaagggtta 2640 tgtgtagtgt tttactgcat aaattatgca
gcaaacaaga caactcaaat taaaaaattt 2700 cctttgcttg tttttttgtt
gtctctgact tgactttctt gtggaagttg gttgtataag 2760 gattgggaca
ccattgtcct tcttaattta attttattct ttgctgataa aaaaaaaaat 2820
ttcatatagt gttaaataat aatttgttaa ataaccaaaa agtcaaatat gtttactctc
2880 gtttaaataa ttgagattcg tccagcaagg ctaaacgatt gtatagattt
atgacaatat 2940 ttactttttt atagataaat gttatattat aataaattta
tatacatata ttatatgtta 3000 tttattatta ttttaaatcc ttcaatattt
tatcaaacca actcataatt ttttttttat 3060 ctgtaagaag caataaaatt
aaatagaccc actttaagga tgatccaacc tttatacaga 3120 gtaagagagt
tcaaatagta ccctttcata tacatatcaa ctaaaatatt agaaatatca 3180
tggatcaaac cttataaaga cattaaataa gtggataagt ataatatata aatgggtagt
3240 atataatata taaatggata caaacttctc tctttataat tgttatgtct
ccttaacatc 3300 ctaatataat acataagtgg gtaatatata atatataaat
ggagacaaac ttcttccatt 3360 ataattgtta tgtcttctta acacttatgt
ctcgttcaca atgctaaggt tagaattgtt 3420 tagaaagtct tatagtacac
atttgttttt gtactatttg aagcattcca taagccgtca 3480 cgattcagat
gatttataat aataagagga aatttatcat agaacaataa ggtgcataga 3540
tagagtgtta atatatcata acatcctttg tttattcata gaagaagtga gatggagctc
3600 agttattata ctgttacatg gtcggataca atattccatg ctctccatga
gctcttacac 3660 ctacatgcat tttagttcat acttgcggcc gcagtatatc
ttaaattctt taatacggtg 3720 tactaggata ttgaactggt tcttgatgat
gaaaacctgg gccgagattg cagctattta 3780 tagtcatagg tcttgttaac
atgcatggac atttggccac ggggtggcat gcagtttgac 3840 gggtgttgaa
ataaacaaaa atgaggtggc ggaagagaat acgagtttga ggttgggtta 3900
gaaacaacaa atgtgagggc tcatgatggg ttgagttggt gaatgttttg ggctgctcga
3960 ttgacacctt tgtgagtacg tgttgttgtg catggctttt ggggtccagt
ttttttttct 4020 tgacgcggcg atcctgatca gctagtggat aagtgatgtc
cactgtgtgt gattgcgttt 4080 ttgtttgaat tttatgaact tagacattgc
tatgcaaagg atactctcat tgtgttttgt 4140 cttcttttgt tccttggctt
tttcttatga tccaagagac tagtcagtgt tgtggcattc 4200 gagactacca
agattaatta tgatggggga aggataagta actgattagt acggactgtt 4260
accaaattaa ttaataagcg gcaaatgaag ggcatggatc aaaagcttgg atctcctgca
4320 ggatctggcc ggccggatct cgtacggatc cgtcgacgg 4359 <210>
SEQ ID NO 85 <211> LENGTH: 5147 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR911 <400> SEQUENCE:
85 ggccgcaagt atgaactaaa atgcatgtag gtgtaagagc tcatggagag
catggaatat 60 tgtatccgac catgtaacag tataataact gagctccatc
tcacttcttc tatgaataaa 120 caaaggatgt tatgatatat taacactcta
tctatgcacc ttattgttct atgataaatt 180 tcctcttatt attataaatc
atctgaatcg tgacggctta tggaatgctt caaatagtac 240 aaaaacaaat
gtgtactata agactttcta aacaattcta accttagcat tgtgaacgag 300
acataagtgt taagaagaca taacaattat aatggaagaa gtttgtctcc atttatatat
360 tatatattac ccacttatgt attatattag gatgttaagg agacataaca
attataaaga 420 gagaagtttg tatccattta tatattatat actacccatt
tatatattat acttatccac 480 ttatttaatg tctttataag gtttgatcca
tgatatttct aatattttag ttgatatgta 540 tatgaaaggg tactatttga
actctcttac tctgtataaa ggttggatca tccttaaagt 600 gggtctattt
aattttattg cttcttacag ataaaaaaaa aattatgagt tggtttgata 660
aaatattgaa ggatttaaaa taataataaa taacatataa tatatgtata taaatttatt
720 ataatataac atttatctat aaaaaagtaa atattgtcat aaatctatac
aatcgtttag 780 ccttgctgga cgaatctcaa ttatttaaac gagagtaaac
atatttgact ttttggttat 840 ttaacaaatt attatttaac actatatgaa
attttttttt ttatcagcaa agaataaaat 900 taaattaaga aggacaatgg
tgtcccaatc cttatacaac caacttccac aagaaagtca 960 agtcagagac
aacaaaaaaa caagcaaagg aaatttttta atttgagttg tcttgtttgc 1020
tgcataattt atgcagtaaa acactacaca taaccctttt agcagtagag caatggttga
1080 ccgtgtgctt agcttctttt attttatttt tttatcagca aagaataaat
aaaataaaat 1140 gagacacttc agggatgttt caacaagctt ggcgcgccgt
tctatagtgt cacctaaatc 1200
gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
1260 ccatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
cagccccgac 1320 acccgccaac acccgctgac gcgccctgac gggcttgtct
gctcccggca tccgcttaca 1380 gacaagctgt gaccgtctcc gggagctgca
tgtgtcagag gttttcaccg tcatcaccga 1440 aacgcgcgag acgaaagggc
ctcgtgatac gcctattttt ataggttaat gtcatgacca 1500 aaatccctta
acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 1560
gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1620 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
ccgaaggtaa 1680 ctggcttcag cagagcgcag ataccaaata ctgtccttct
agtgtagccg tagttaggcc 1740 accacttcaa gaactctgta gcaccgccta
catacctcgc tctgctaatc ctgttaccag 1800 tggctgctgc cagtggcgat
aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 1860 cggataaggc
gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 1920
gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
1980 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
ggagagcgca 2040 cgagggagct tccaggggga aacgcctggt atctttatag
tcctgtcggg tttcgccacc 2100 tctgacttga gcgtcgattt ttgtgatgct
cgtcaggggg gcggagccta tggaaaaacg 2160 ccagcaacgc ggccttttta
cggttcctgg ccttttgctg gccttttgct cacatgttct 2220 ttcctgcgtt
atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 2280
ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
2340 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
aggttgatca 2400 gatctcgatc ccgcgaaatt aatacgactc actataggga
gaccacaacg gtttccctct 2460 agaaataatt ttgtttaact ttaagaagga
gatataccca tggaaaagcc tgaactcacc 2520 gcgacgtctg tcgagaagtt
tctgatcgaa aagttcgaca gcgtctccga cctgatgcag 2580 ctctcggagg
gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc 2640
ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
2700 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
cgagagcctg 2760 acctattgca tctcccgccg tgcacagggt gtcacgttgc
aagacctgcc tgaaaccgaa 2820 ctgcccgctg ttctgcagcc ggtcgcggag
gctatggatg cgatcgctgc ggccgatctt 2880 agccagacga gcgggttcgg
cccattcgga ccgcaaggaa tcggtcaata cactacatgg 2940 cgtgatttca
tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac 3000
gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
3060 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
cctgacggac 3120 aatggccgca taacagcggt cattgactgg agcgaggcga
tgttcgggga ttcccaatac 3180 gaggtcgcca acatcttctt ctggaggccg
tggttggctt gtatggagca gcagacgcgc 3240 tacttcgagc ggaggcatcc
ggagcttgca ggatcgccgc ggctccgggc gtatatgctc 3300 cgcattggtc
ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct 3360
tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
3420 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
actcgccgat 3480 agtggaaacc gacgccccag cactcgtccg agggcaaagg
aatagtgagg tacagcttgg 3540 atcgatccgg ctgctaacaa agcccgaaag
gaagctgagt tggctgctgc caccgctgag 3600 caataactag cataacccct
tggggcctct aaacgggtct tgaggggttt tttgctgaaa 3660 ggaggaacta
tatccggatg atcgggcgcg ccgtcgacgg atccgtacga gatccggccg 3720
gccagatcct gcaggagatc caagcttttg atccatgccc ttcatttgcc gcttattaat
3780 taatttggta acagtccgta ctaatcagtt acttatcctt cccccatcat
aattaatctt 3840 ggtagtctcg aatgccacaa cactgactag tctcttggat
cataagaaaa agccaaggaa 3900 caaaagaaga caaaacacaa tgagagtatc
ctttgcatag caatgtctaa gttcataaaa 3960 ttcaaacaaa aacgcaatca
cacacagtgg acatcactta tccactagct gatcaggatc 4020 gccgcgtcaa
gaaaaaaaaa ctggacccca aaagccatgc acaacaacac gtactcacaa 4080
aggtgtcaat cgagcagccc aaaacattca ccaactcaac ccatcatgag ccctcacatt
4140 tgttgtttct aacccaacct caaactcgta ttctcttccg ccacctcatt
tttgtttatt 4200 tcaacacccg tcaaactgca tgccaccccg tggccaaatg
tccatgcatg ttaacaagac 4260 ctatgactat aaatagctgc aatctcggcc
caggttttca tcatcaagaa ccagttcaat 4320 atcctagtac accgtattaa
agaatttaag atatactgcg gccgcaccat ggaggtggtg 4380 aatgaaatag
tctcaattgg gcaggaagtt ttacccaaag ttgattatgc ccaactctgg 4440
agtgatgcca gtcactgtga ggtgctttac ttgtccatcg catttgtcat cttgaagttc
4500 actcttggcc cccttggtcc aaaaggtcag tctcgtatga agtttgtttt
caccaattac 4560 aaccttctca tgtccattta ttcgttggga tcattcctct
caatggcata tgccatgtac 4620 accatcggtg ttatgtctga caactgcgag
aaggcttttg acaacaacgt cttcaggatc 4680 accacgcagt tgttctattt
gagcaagttc ctggagtata ttgactcctt ctatttgcca 4740 ctgatgggca
agcctctgac ctggttgcaa ttcttccatc atttgggggc accgatggat 4800
atgtggctgt tctataatta ccgaaatgaa gctgtttgga tttttgtgct gttgaatggt
4860 ttcatccact ggatcatgta cggttattat tggaccagat tgatcaagct
gaagttcccc 4920 atgccaaaat ccctgattac atcaatgcag atcattcaat
tcaatgttgg tttctacatt 4980 gtctggaagt acaggaacat tccctgttat
cgccaagatg ggatgaggat gtttggctgg 5040 ttcttcaatt acttttatgt
tggcacagtc ttgtgtttgt tcttgaattt ctatgtgcaa 5100 acgtatatcg
tcaggaagca caagggagcc aaaaagattc agtgagc 5147 <210> SEQ ID NO
86 <211> LENGTH: 6559 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR680 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (4340)..(4340)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 86 ggccgcgaca caagtgtgag agtactaaat aaatgctttg gttgtacgaa
atcattacac 60 taaataaaat aatcaaagct tatatatgcc ttccgctaag
gccgaatgca aagaaattgg 120 ttctttctcg ttatcttttg ccacttttac
tagtacgtat taattactac ttaatcatct 180 ttgtttacgg ctcattatat
ccggtctaga ggatccaagg ccgcgaagtt aaaagcaatg 240 ttgtcacttg
tcgtactaac acatgatgtg atagtttatg ctagctagct ataacataag 300
ctgtctctga gtgtgttgta tattaataaa gatcatcact ggtgaatggt gatcgtgtac
360 gtaccctact tagtaggcaa tggaagcact tagagtgtgc tttgtgcatg
gccttgcctc 420 tgttttgaga cttttgtaat gttttcgagt ttaaatcttt
gcctttgcgt acgtgggcgg 480 atcccccggg ctgcaggaat tcactggccg
tcgttttaca acgtcgtgac tgggaaaacc 540 ctggcgttac ccaacttaat
cgccttgcag cacatccccc tttcgccagc tggcgtaata 600 gcgaagaggc
ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 660
gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
720 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
ccgccaacac 780 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
cgcttacaga caagctgtga 840 ccgtctccgg gagctgcatg tgtcagaggt
tttcaccgtc atcaccgaaa cgcgcgagac 900 gaaagggcct cgtgatacgc
ctatttttat aggttaatgt catgataata atggtttctt 960 agacgtcagg
tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 1020
aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
1080 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
cccttttttg 1140 cggcattttg ccttcctgtt tttgctcacc cagaaacgct
ggtgaaagta aaagatgctg 1200 aagatcagtt gggtgcacga gtgggttaca
tcgaactgga tctcaacagc ggtaagatcc 1260 ttgagagttt tcgccccgaa
gaacgttttc caatgatgag cacttttaaa gttctgctat 1320 gtggcgcggt
attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 1380
attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
1440 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
gcggccaact 1500 tacttctgac aacgatcgga ggaccgaagg agctaaccgc
ttttttgcac aacatggggg 1560 atcatgtaac tcgccttgat cgttgggaac
cggagctgaa tgaagccata ccaaacgacg 1620 agcgtgacac cacgatgcct
gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 1680 aactacttac
tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 1740
caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
1800 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
aagccctccc 1860 gtatcgtagt tatctacacg acggggagtc aggcaactat
ggatgaacga aatagacaga 1920 tcgctgagat aggtgcctca ctgattaagc
attggtaact gtcagaccaa gtttactcat 1980 atatacttta gattgattta
aaacttcatt tttaatttaa aaggatctag gtgaagatcc 2040 tttttgataa
tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 2100
accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
2160 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
caagagctac 2220 caactctttt tccgaaggta actggcttca gcagagcgca
gataccaaat actgtccttc 2280 tagtgtagcc gtagttaggc caccacttca
agaactctgt agcaccgcct acatacctcg 2340 ctctgctaat cctgttacca
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2400 tggactcaag
acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 2460
gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
2520 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
gtaagcggca 2580 gggtcggaac aggagagcgc acgagggagc ttccaggggg
aaacgcctgg tatctttata 2640 gtcctgtcgg gtttcgccac ctctgacttg
agcgtcgatt tttgtgatgc tcgtcagggg 2700 ggcggagcct atggaaaaac
gccagcaacg cggccttttt acggttcctg gccttttgct 2760 ggccttttgc
tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 2820
ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
2880 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
cgttggccga 2940 ttcattaatg cagctggcac gacaggtttc ccgactggaa
agcgggcagt gagcgcaacg 3000 caattaatgt gagttagctc actcattagg
caccccaggc tttacacttt atgcttccgg 3060 ctcgtatgtt gtgtggaatt
gtgagcggat aacaatttca cacaggaaac agctatgacc 3120 atgattacgc
caagcttgca tgcctgcagg tcgactcgac gtacgtcctc gaagagaagg 3180
gttaataaca cattttttaa catttttaac acaaatttta gttatttaaa aatttattaa
3240 aaaatttaaa ataagaagag gaactcttta aataaatcta acttacaaaa
tttatgattt 3300 ttaataagtt ttcaccaata aaaaatgtca taaaaatatg
ttaaaaagta tattatcaat 3360 attctcttta tgataaataa aaagaaaaaa
aaaataaaag ttaagtgaaa atgagattga 3420 agtgacttta ggtgtgtata
aatatatcaa ccccgccaac aatttattta atccaaatat 3480 attgaagtat
attattccat agcctttatt tatttatata tttattatat aaaagcttta 3540
tttgttctag gttgttcatg aaatattttt ttggttttat ctccgttgta agaaaatcat
3600 gtgctttgtg tcgccactca ctattgcagc tttttcatgc attggtcaga
ttgacggttg 3660 attgtatttt tgttttttat ggttttgtgt tatgacttaa
gtcttcatct ctttatctct 3720 tcatcaggtt tgatggttac ctaatatggt
ccatgggtac atgcatggtt aaattaggtg 3780 gccaactttg ttgtgaacga
tagaattttt tttatattaa gtaaactatt tttatattat 3840 gaaataataa
taaaaaaaat attttatcat tattaacaaa atcatattag ttaatttgtt 3900
aactctataa taaaagaaat actgtaacat tcacattaca tggtaacatc tttccaccct
3960 ttcatttgtt ttttgtttga tgactttttt tcttgtttaa atttatttcc
cttcttttaa 4020 atttggaata cattatcatc atatataaac taaaatacta
aaaacaggat tacacaaatg 4080 ataaataata acacaaatat ttataaatct
agctgcaata tatttaaact agctatatcg 4140 atattgtaaa ataaaactag
ctgcattgat actgataaaa aaatatcatg tgctttctgg 4200 actgatgatg
cagtatactt ttgacattgc ctttatttta tttttcagaa aagctttctt 4260
agttctgggt tcttcattat ttgtttccca tctccattgt gaattgaatc atttgcttcg
4320 tgtcacaaat acaatttagn taggtacatg cattggtcag attcacggtt
tattatgtca 4380 tgacttaagt tcatggtagt acattacctg ccacgcatgc
attatattgg ttagatttga 4440 taggcaaatt tggttgtcaa caatataaat
ataaataatg tttttatatt acgaaataac 4500 agtgatcaaa acaaacagtt
ttatctttat taacaagatt ttgtttttgt ttgatgacgt 4560 tttttaatgt
ttacgctttc ccccttcttt tgaatttaga acactttatc atcataaaat 4620
caaatactaa aaaaattaca tatttcataa ataataacac aaatattttt aaaaaatctg
4680 aaataataat gaacaatatt acatattatc acgaaaattc attaataaaa
atattatata 4740 aataaaatgt aatagtagtt atatgtagga aaaaagtact
gcacgcataa tatatacaaa 4800 aagattaaaa tgaactatta taaataataa
cactaaatta atggtgaatc atatcaaaat 4860 aatgaaaaag taaataaaat
ttgtaattaa cttctatatg tattacacac acaaataata 4920 aataatagta
aaaaaaatta tgataaatat ttaccatctc ataagatatt taaaataatg 4980
ataaaaatat agattatttt ttatgcaact agctagccaa aaagagaaca cgggtatata
5040 taaaaagagt acctttaaat tctactgtac ttcctttatt cctgacgttt
ttatatcaag 5100 tggacatacg tgaagatttt aattatcagt ctaaatattt
cattagcact taatactttt 5160 ctgttttatt cctatcctat aagtagtccc
gattctccca acattgctta ttcacacaac 5220 taactaagaa agtcttccat
agccccccaa gcggccgcgg gaattcgatt gaaatgaagt 5280 caaagcgcca
agcgcttccc cttacaattg atggaacaac atatgatgtg tctgcctggg 5340
tcaatttcca ccctggtggt gcggaaatta tagagaatta ccaaggaagg gatgccactg
5400 atgccttcat ggttatgcac tctcaagaag ccttcgacaa gctcaagcgc
atgcccaaaa 5460 tcaatcccag ttctgagttg ccaccccagg ctgcagtgaa
tgaagctcaa gaggatttcc 5520 ggaagctccg agaagagttg atcgcaactg
gcatgtttga tgcctccccc ctctggtact 5580 catacaaaat cagcaccaca
ctgggccttg gagtgctggg ttatttcctg atggttcagt 5640 atcagatgta
tttcattggg gcagtgttgc ttgggatgca ctatcaacag atgggctggc 5700
tttctcatga catttgccac caccagactt tcaagaaccg gaactggaac aacctcgtgg
5760 gactggtatt tggcaatggt ctgcaaggtt tttccgtgac atggtggaag
gacagacaca 5820 atgcacatca ttcggcaacc aatgttcaag ggcacgaccc
tgatattgac aacctccccc 5880 tcttagcctg gtctgaggat gacgtcacac
gggcgtcacc gatttcccgc aagctcattc 5940 agttccagca gtactatttc
ttggtcatct gtatcttgtt gcggttcatt tggtgtttcc 6000 agagcgtgtt
gaccgtgcgc agtttgaagg acagagataa ccaattctat cgctctcagt 6060
ataagaagga ggccattggc ctcgccctgc actggacctt gaagaccctg ttccacttat
6120 tctttatgcc cagcatcctc acatcgctgt tggtgttttt cgtttcggag
ctggttggcg 6180 gcttcggcat tgcgatcgtg gtgttcatga accactaccc
actggagaag atcggggact 6240 cagtctggga tggccatgga ttctcggttg
gccagatcca tgagaccatg aacattcggc 6300 gagggattat cacagattgg
tttttcggag gcttgaatta ccagattgag caccatttgt 6360 ggccgaccct
ccctcgccac aacctgacag cggttagcta ccaggtggaa cagctgtgcc 6420
agaagcacaa cctgccgtat cggaacccgc tgccccatga agggttggtc atcctgctgc
6480 gctatctggc ggtgttcgcc cggatggcgg agaagcaacc cgcggggaag
gctctataag 6540 gaatcactag tgaattcgc 6559 <210> SEQ ID NO 87
<211> LENGTH: 9014 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKR913 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (7839)..(7839)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 87 gtacgagatc cggccggcca gatcctgcag gagatccaag cttttgatcc
atgcccttca 60 tttgccgctt attaattaat ttggtaacag tccgtactaa
tcagttactt atccttcccc 120 catcataatt aatcttggta gtctcgaatg
ccacaacact gactagtctc ttggatcata 180 agaaaaagcc aaggaacaaa
agaagacaaa acacaatgag agtatccttt gcatagcaat 240 gtctaagttc
ataaaattca aacaaaaacg caatcacaca cagtggacat cacttatcca 300
ctagctgatc aggatcgccg cgtcaagaaa aaaaaactgg accccaaaag ccatgcacaa
360 caacacgtac tcacaaaggt gtcaatcgag cagcccaaaa cattcaccaa
ctcaacccat 420 catgagccct cacatttgtt gtttctaacc caacctcaaa
ctcgtattct cttccgccac 480 ctcatttttg tttatttcaa cacccgtcaa
actgcatgcc accccgtggc caaatgtcca 540 tgcatgttaa caagacctat
gactataaat agctgcaatc tcggcccagg ttttcatcat 600 caagaaccag
ttcaatatcc tagtacaccg tattaaagaa tttaagatat actgcggccg 660
caccatggag gtggtgaatg aaatagtctc aattgggcag gaagttttac ccaaagttga
720 ttatgcccaa ctctggagtg atgccagtca ctgtgaggtg ctttacttgt
ccatcgcatt 780 tgtcatcttg aagttcactc ttggccccct tggtccaaaa
ggtcagtctc gtatgaagtt 840 tgttttcacc aattacaacc ttctcatgtc
catttattcg ttgggatcat tcctctcaat 900 ggcatatgcc atgtacacca
tcggtgttat gtctgacaac tgcgagaagg cttttgacaa 960 caacgtcttc
aggatcacca cgcagttgtt ctatttgagc aagttcctgg agtatattga 1020
ctccttctat ttgccactga tgggcaagcc tctgacctgg ttgcaattct tccatcattt
1080 gggggcaccg atggatatgt ggctgttcta taattaccga aatgaagctg
tttggatttt 1140 tgtgctgttg aatggtttca tccactggat catgtacggt
tattattgga ccagattgat 1200 caagctgaag ttccccatgc caaaatccct
gattacatca atgcagatca ttcaattcaa 1260 tgttggtttc tacattgtct
ggaagtacag gaacattccc tgttatcgcc aagatgggat 1320 gaggatgttt
ggctggttct tcaattactt ttatgttggc acagtcttgt gtttgttctt 1380
gaatttctat gtgcaaacgt atatcgtcag gaagcacaag ggagccaaaa agattcagtg
1440 agcggccgca agtatgaact aaaatgcatg taggtgtaag agctcatgga
gagcatggaa 1500 tattgtatcc gaccatgtaa cagtataata actgagctcc
atctcacttc ttctatgaat 1560 aaacaaagga tgttatgata tattaacact
ctatctatgc accttattgt tctatgataa 1620 atttcctctt attattataa
atcatctgaa tcgtgacggc ttatggaatg cttcaaatag 1680 tacaaaaaca
aatgtgtact ataagacttt ctaaacaatt ctaaccttag cattgtgaac 1740
gagacataag tgttaagaag acataacaat tataatggaa gaagtttgtc tccatttata
1800 tattatatat tacccactta tgtattatat taggatgtta aggagacata
acaattataa 1860 agagagaagt ttgtatccat ttatatatta tatactaccc
atttatatat tatacttatc 1920 cacttattta atgtctttat aaggtttgat
ccatgatatt tctaatattt tagttgatat 1980 gtatatgaaa gggtactatt
tgaactctct tactctgtat aaaggttgga tcatccttaa 2040 agtgggtcta
tttaatttta ttgcttctta cagataaaaa aaaaattatg agttggtttg 2100
ataaaatatt gaaggattta aaataataat aaataacata taatatatgt atataaattt
2160 attataatat aacatttatc tataaaaaag taaatattgt cataaatcta
tacaatcgtt 2220 tagccttgct ggacgaatct caattattta aacgagagta
aacatatttg actttttggt 2280 tatttaacaa attattattt aacactatat
gaaatttttt tttttatcag caaagaataa 2340 aattaaatta agaaggacaa
tggtgtccca atccttatac aaccaacttc cacaagaaag 2400 tcaagtcaga
gacaacaaaa aaacaagcaa aggaaatttt ttaatttgag ttgtcttgtt 2460
tgctgcataa tttatgcagt aaaacactac acataaccct tttagcagta gagcaatggt
2520 tgaccgtgtg cttagcttct tttattttat ttttttatca gcaaagaata
aataaaataa 2580 aatgagacac ttcagggatg tttcaacaag cttggcgcgc
cgttctatag tgtcacctaa 2640 atcgtatgtg tatgatacat aaggttatgt
attaattgta gccgcgttct aacgacaata 2700 tgtccatatg gtgcactctc
agtacaatct gctctgatgc cgcatagtta agccagcccc 2760 gacacccgcc
aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2820
acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
2880 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
aatgtcatga 2940 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
agaccccgta gaaaagatca 3000 aaggatcttc ttgagatcct ttttttctgc
gcgtaatctg ctgcttgcaa acaaaaaaac 3060 caccgctacc agcggtggtt
tgtttgccgg atcaagagct accaactctt tttccgaagg 3120 taactggctt
cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 3180
gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
3240 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
agacgatagt 3300 taccggataa ggcgcagcgg tcgggctgaa cggggggttc
gtgcacacag cccagcttgg 3360 agcgaacgac ctacaccgaa ctgagatacc
tacagcgtga gcattgagaa agcgccacgc 3420 ttcccgaagg gagaaaggcg
gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3480 gcacgaggga
gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3540
acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
3600 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
gctcacatgt 3660
tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
3720 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
gaagcggaag 3780 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
gattcattaa tgcaggttga 3840 tcagatctcg atcccgcgaa attaatacga
ctcactatag ggagaccaca acggtttccc 3900 tctagaaata attttgttta
actttaagaa ggagatatac ccatggaaaa gcctgaactc 3960 accgcgacgt
ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg 4020
cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
4080 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
ttatcggcac 4140 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
ttggggaatt cagcgagagc 4200 ctgacctatt gcatctcccg ccgtgcacag
ggtgtcacgt tgcaagacct gcctgaaacc 4260 gaactgcccg ctgttctgca
gccggtcgcg gaggctatgg atgcgatcgc tgcggccgat 4320 cttagccaga
cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca 4380
tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
4440 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
ttgggccgag 4500 gactgccccg aagtccggca cctcgtgcac gcggatttcg
gctccaacaa tgtcctgacg 4560 gacaatggcc gcataacagc ggtcattgac
tggagcgagg cgatgttcgg ggattcccaa 4620 tacgaggtcg ccaacatctt
cttctggagg ccgtggttgg cttgtatgga gcagcagacg 4680 cgctacttcg
agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg 4740
ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
4800 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
tgtcgggcgt 4860 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
gctgtgtaga agtactcgcc 4920 gatagtggaa accgacgccc cagcactcgt
ccgagggcaa aggaatagtg aggtacagct 4980 tggatcgatc cggctgctaa
caaagcccga aaggaagctg agttggctgc tgccaccgct 5040 gagcaataac
tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg 5100
aaaggaggaa ctatatccgg atgatcgggc gcgccgtcga cggatccgta cgcaaaggca
5160 aagatttaaa ctcgaaaaca ttacaaaagt ctcaaaacag aggcaaggcc
atgcacaaag 5220 cacactctaa gtgcttccat tgcctactaa gtagggtacg
tacacgatca ccattcacca 5280 gtgatgatct ttattaatat acaacacact
cagagacagc ttatgttata gctagctagc 5340 ataaactatc acatcatgtg
ttagtacgac aagtgacaac attgctttta acttcgcggc 5400 cttggatcct
ctagaccgga tataatgagc cgtaaacaaa gatgattaag tagtaattaa 5460
tacgtactag taaaagtggc aaaagataac gagaaagaac caatttcttt gcattcggcc
5520 ttagcggaag gcatatataa gctttgatta ttttatttag tgtaatgatt
tcgtacaacc 5580 aaagcattta tttagtactc tcacacttgt gtcgcggccg
cgaattcact agtgattcct 5640 tatagagcct tccccgcggg ttgcttctcc
gccatccggg cgaacaccgc cagatagcgc 5700 agcaggatga ccaacccttc
atggggcagc gggttccgat acggcaggtt gtgcttctgg 5760 cacagctgtt
ccacctggta gctaaccgct gtcaggttgt ggcgagggag ggtcggccac 5820
aaatggtgct caatctggta attcaagcct ccgaaaaacc aatctgtgat aatccctcgc
5880 cgaatgttca tggtctcatg gatctggcca accgagaatc catggccatc
ccagactgag 5940 tccccgatct tctccagtgg gtagtggttc atgaacacca
cgatcgcaat gccgaagccg 6000 ccaaccagct ccgaaacgaa aaacaccaac
agcgatgtga ggatgctggg cataaagaat 6060 aagtggaaca gggtcttcaa
ggtccagtgc agggcgaggc caatggcctc cttcttatac 6120 tgagagcgat
agaattggtt atctctgtcc ttcaaactgc gcacggtcaa cacgctctgg 6180
aaacaccaaa tgaaccgcaa caagatacag atgaccaaga aatagtactg ctggaactga
6240 atgagcttgc gggaaatcgg tgacgcccgt gtgacgtcat cctcagacca
ggctaagagg 6300 gggaggttgt caatatcagg gtcgtgccct tgaacattgg
ttgccgaatg atgtgcattg 6360 tgtctgtcct tccaccatgt cacggaaaaa
ccttgcagac cattgccaaa taccagtccc 6420 acgaggttgt tccagttccg
gttcttgaaa gtctggtggt ggcaaatgtc atgagaaagc 6480 cagcccatct
gttgatagtg catcccaagc aacactgccc caatgaaata catctgatac 6540
tgaaccatca ggaaataacc cagcactcca aggcccagtg tggtgctgat tttgtatgag
6600 taccagaggg gggaggcatc aaacatgcca gttgcgatca actcttctcg
gagcttccgg 6660 aaatcctctt gagcttcatt cactgcagcc tggggtggca
actcagaact gggattgatt 6720 ttgggcatgc gcttgagctt gtcgaaggct
tcttgagagt gcataaccat gaaggcatca 6780 gtggcatccc ttccttggta
attctctata atttccgcac caccagggtg gaaattgacc 6840 caggcagaca
catcatatgt tgttccatca attgtaaggg gaagcgcttg gcgctttgac 6900
ttcatttcaa tcgaattccc gcggccgctt ggggggctat ggaagacttt cttagttagt
6960 tgtgtgaata agcaatgttg ggagaatcgg gactacttat aggataggaa
taaaacagaa 7020 aagtattaag tgctaatgaa atatttagac tgataattaa
aatcttcacg tatgtccact 7080 tgatataaaa acgtcaggaa taaaggaagt
acagtagaat ttaaaggtac tctttttata 7140 tatacccgtg ttctcttttt
ggctagctag ttgcataaaa aataatctat atttttatca 7200 ttattttaaa
tatcttatga gatggtaaat atttatcata atttttttta ctattattta 7260
ttatttgtgt gtgtaataca tatagaagtt aattacaaat tttatttact ttttcattat
7320 tttgatatga ttcaccatta atttagtgtt attatttata atagttcatt
ttaatctttt 7380 tgtatatatt atgcgtgcag tacttttttc ctacatataa
ctactattac attttattta 7440 tataatattt ttattaatga attttcgtga
taatatgtaa tattgttcat tattatttca 7500 gattttttaa aaatatttgt
gttattattt atgaaatatg taattttttt agtatttgat 7560 tttatgatga
taaagtgttc taaattcaaa agaaggggga aagcgtaaac attaaaaaac 7620
gtcatcaaac aaaaacaaaa tcttgttaat aaagataaaa ctgtttgttt tgatcactgt
7680 tatttcgtaa tataaaaaca ttatttatat ttatattgtt gacaaccaaa
tttgcctatc 7740 aaatctaacc aatataatgc atgcgtggca ggtaatgtac
taccatgaac ttaagtcatg 7800 acataataaa ccgtgaatct gaccaatgca
tgtacctanc taaattgtat ttgtgacacg 7860 aagcaaatga ttcaattcac
aatggagatg ggaaacaaat aatgaagaac ccagaactaa 7920 gaaagctttt
ctgaaaaata aaataaaggc aatgtcaaaa gtatactgca tcatcagtcc 7980
agaaagcaca tgatattttt ttatcagtat caatgcagct agttttattt tacaatatcg
8040 atatagctag tttaaatata ttgcagctag atttataaat atttgtgtta
ttatttatca 8100 tttgtgtaat cctgttttta gtattttagt ttatatatga
tgataatgta ttccaaattt 8160 aaaagaaggg aaataaattt aaacaagaaa
aaaagtcatc aaacaaaaaa caaatgaaag 8220 ggtggaaaga tgttaccatg
taatgtgaat gttacagtat ttcttttatt atagagttaa 8280 caaattaact
aatatgattt tgttaataat gataaaatat tttttttatt attatttcat 8340
aatataaaaa tagtttactt aatataaaaa aaattctatc gttcacaaca aagttggcca
8400 cctaatttaa ccatgcatgt acccatggac catattaggt aaccatcaaa
cctgatgaag 8460 agataaagag atgaagactt aagtcataac acaaaaccat
aaaaaacaaa aatacaatca 8520 accgtcaatc tgaccaatgc atgaaaaagc
tgcaatagtg agtggcgaca caaagcacat 8580 gattttctta caacggagat
aaaaccaaaa aaatatttca tgaacaacct agaacaaata 8640 aagcttttat
ataataaata tataaataaa taaaggctat ggaataatat acttcaatat 8700
atttggatta aataaattgt tggcggggtt gatatattta tacacaccta aagtcacttc
8760 aatctcattt tcacttaact tttatttttt ttttcttttt atttatcata
aagagaatat 8820 tgataatata ctttttaaca tatttttatg acatttttta
ttggtgaaaa cttattaaaa 8880 atcataaatt ttgtaagtta gatttattta
aagagttcct cttcttattt taaatttttt 8940 aataaatttt taaataacta
aaatttgtgt taaaaatgtt aaaaaatgtg ttattaaccc 9000 ttctcttcga ggac
9014 <210> SEQ ID NO 88 <211> LENGTH: 5561 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pKR767 <400>
SEQUENCE: 88 catggtcaat caatgagacg ccaacttctt aatctattga gacctgcagg
tctagaaggg 60 cggatccccg ggtaccgagc tcgaattcac tggccgtcgt
tttacaacgt cgtgactggg 120 aaaaccctgg cgttacccaa cttaatcgcc
ttgcagcaca tccccctttc gccagctggc 180 gtaatagcga agaggcccgc
accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 240 aatggcgcct
gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 300
ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
360 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
tacagacaag 420 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
accgtcatca ccgaaacgcg 480 cgagacgaaa gggcctcgtg atacgcctat
ttttataggt taatgtcatg ataataatgg 540 tttcttagac gtcaggtggc
acttttcggg gaaatgtgcg cggaacccct atttgtttat 600 ttttctaaat
acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 660
aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
720 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
aaagtaaaag 780 atgctgaaga tcagttgggt gcacgagtgg gttacatcga
actggatctc aacagcggta 840 agatccttga gagttttcgc cccgaagaac
gttttccaat gatgagcact tttaaagttc 900 tgctatgtgg cgcggtatta
tcccgtattg acgccgggca agagcaactc ggtcgccgca 960 tacactattc
tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 1020
atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
1080 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
ttgcacaaca 1140 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
gctgaatgaa gccataccaa 1200 acgacgagcg tgacaccacg atgcctgtag
caatggcaac aacgttgcgc aaactattaa 1260 ctggcgaact acttactcta
gcttcccggc aacaattaat agactggatg gaggcggata 1320 aagttgcagg
accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 1380
ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
1440 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
gaacgaaata 1500 gacagatcgc tgagataggt gcctcactga ttaagcattg
gtaactgtca gaccaagttt 1560 actcatatat actttagatt gatttaaaac
ttcattttta atttaaaagg atctaggtga 1620 agatcctttt tgataatctc
atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1680 cgtcagaccc
cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1740
tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
1800 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
ccaaatactg 1860
tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
1920 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
tcgtgtctta 1980 ccgggttgga ctcaagacga tagttaccgg ataaggcgca
gcggtcgggc tgaacggggg 2040 gttcgtgcac acagcccagc ttggagcgaa
cgacctacac cgaactgaga tacctacagc 2100 gtgagctatg agaaagcgcc
acgcttcccg aagggagaaa ggcggacagg tatccggtaa 2160 gcggcagggt
cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 2220
tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
2280 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
ttcctggcct 2340 tttgctggcc ttttgctcac atgttctttc ctgcgttatc
ccctgattct gtggataacc 2400 gtattaccgc ctttgagtga gctgataccg
ctcgccgcag ccgaacgacc gagcgcagcg 2460 agtcagtgag cgaggaagcg
gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 2520 ggccgattca
ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 2580
gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
2640 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
ggaaacagct 2700 atgaccatga ttacgccaag cttgcatgcc tgcaggctag
cctaagtacg tactcaaaat 2760 gccaacaaat aaaaaaaaag ttgctttaat
aatgccaaaa caaattaata aaacacttac 2820 aacaccggat tttttttaat
taaaatgtgc catttaggat aaatagttaa tatttttaat 2880 aattatttaa
aaagccgtat ctactaaaat gatttttatt tggttgaaaa tattaatatg 2940
tttaaatcaa cacaatctat caaaattaaa ctaaaaaaaa aataagtgta cgtggttaac
3000 attagtacag taatataaga ggaaaatgag aaattaagaa attgaaagcg
agtctaattt 3060 ttaaattatg aacctgcata tataaaagga aagaaagaat
ccaggaagaa aagaaatgaa 3120 accatgcatg gtcccctcgt catcacgagt
ttctgccatt tgcaatagaa acactgaaac 3180 acctttctct ttgtcactta
attgagatgc cgaagccacc tcacaccatg aacttcatga 3240 ggtgtagcac
ccaaggcttc catagccatg catactgaag aatgtctcaa gctcagcacc 3300
ctacttctgt gacgtgtccc tcattcacct tcctctcttc cctataaata accacgcctc
3360 aggttctccg cttcacaact caaacattct ctccattggt ccttaaacac
tcatcagtca 3420 tcaccgcggc cgcatgggaa cggaccaagg aaaaaccttc
acctgggaag agctggcggc 3480 ccataacacc aaggacgacc tactcttggc
catccgcggc agggtgtacg atgtcacaaa 3540 gttcttgagc cgccatcctg
gtggagtgga cactctcctg ctcggagctg gccgagatgt 3600 tactccggtc
tttgagatgt atcacgcgtt tggggctgca gatgccatta tgaagaagta 3660
ctatgtcggt acactggtct cgaatgagct gcccatcttc ccggagccaa cggtgttcca
3720 caaaaccatc aagacgagag tcgagggcta ctttacggat cggaacattg
atcccaagaa 3780 tagaccagag atctggggac gatacgctct tatctttgga
tccttgatcg cttcctacta 3840 cgcgcagctc tttgtgcctt tcgttgtcga
acgcacatgg cttcaggtgg tgtttgcaat 3900 catcatggga tttgcgtgcg
cacaagtcgg actcaaccct cttcatgatg cgtctcactt 3960 ttcagtgacc
cacaacccca ctgtctggaa gattctggga gccacgcacg actttttcaa 4020
cggagcatcg tacctggtgt ggatgtacca acatatgctc ggccatcacc cctacaccaa
4080 cattgctgga gcagatcccg acgtgtcgac gtctgagccc gatgttcgtc
gtatcaagcc 4140 caaccaaaag tggtttgtca accacatcaa ccagcacatg
tttgttcctt tcctgtacgg 4200 actgctggcg ttcaaggtgc gcattcagga
catcaacatt ttgtactttg tcaagaccaa 4260 tgacgctatt cgtgtcaatc
ccatctcgac atggcacact gtgatgttct ggggcggcaa 4320 ggctttcttt
gtctggtatc gcctgattgt tcccctgcag tatctgcccc tgggcaaggt 4380
gctgctcttg ttcacggtcg cggacatggt gtcgtcttac tggctggcgc tgaccttcca
4440 ggcgaaccac gttgttgagg aagttcagtg gccgttgcct gacgagaacg
ggatcatcca 4500 aaaggactgg gcagctatgc aggtcgagac tacgcaggat
tacgcacacg attcgcacct 4560 ctggaccagc atcactggca gcttgaacta
ccaggctgtg caccatctgt tccccaacgt 4620 gtcgcagcac cattatcccg
atattctggc catcatcaag aacacctgca gcgagtacaa 4680 ggttccatac
cttgtcaagg atacgttttg gcaagcattt gcttcacatt tggagcactt 4740
gcgtgttctt ggactccgtc ccaaggaaga gtaggcggcc gcatttcgca ccaaatcaat
4800 gaaagtaata atgaaaagtc tgaataagaa tacttaggct tagatgcctt
tgttacttgt 4860 gtaaaataac ttgagtcatg tacctttggc ggaaacagaa
taaataaaag gtgaaattcc 4920 aatgctctat gtataagtta gtaatactta
atgtgttcta cggttgtttc aatatcatca 4980 aactctaatt gaaactttag
aaccacaaat ctcaatcttt tcttaatgaa atgaaaaatc 5040 ttaattgtac
catgtttatg ttaaacacct tacaattggt tggagaggag gaccaaccga 5100
tgggacaaca ttgggagaaa gagattcaat ggagatttgg ataggagaac aacattcttt
5160 ttcacttcaa tacaagatga gtgcaacact aaggatatgt atgagacttt
cagaagctac 5220 gacaacatag atgagtgagg tggtgattcc tagcaagaaa
gacattagag gaagccaaaa 5280 tcgaacaagg aagacatcaa gggcaagaga
caggaccatc catctcagga aaaggagctt 5340 tgggatagtc cgagaagttg
tacaagaaat tttttggagg gtgagtgatg cattgctggt 5400 gactttaact
caatcaaaat tgagaaagaa agaaaaggga gggggctcac atgtgaatag 5460
aagggaaacg ggagaatttt acagttttga tctaatgggc atcccagcta gtggtaacat
5520 attcaccatg tttaaccttc acgtacgtct agaggatccc c 5561 <210>
SEQ ID NO 89 <211> LENGTH: 11889 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR916 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(7810)..(7810) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 89 ggagatccaa gcttttgatc catgcccttc
atttgccgct tattaattaa tttggtaaca 60 gtccgtacta atcagttact
tatccttccc ccatcataat taatcttggt agtctcgaat 120 gccacaacac
tgactagtct cttggatcat aagaaaaagc caaggaacaa aagaagacaa 180
aacacaatga gagtatcctt tgcatagcaa tgtctaagtt cataaaattc aaacaaaaac
240 gcaatcacac acagtggaca tcacttatcc actagctgat caggatcgcc
gcgtcaagaa 300 aaaaaaactg gaccccaaaa gccatgcaca acaacacgta
ctcacaaagg tgtcaatcga 360 gcagcccaaa acattcacca actcaaccca
tcatgagccc tcacatttgt tgtttctaac 420 ccaacctcaa actcgtattc
tcttccgcca cctcattttt gtttatttca acacccgtca 480 aactgcatgc
caccccgtgg ccaaatgtcc atgcatgtta acaagaccta tgactataaa 540
tagctgcaat ctcggcccag gttttcatca tcaagaacca gttcaatatc ctagtacacc
600 gtattaaaga atttaagata tactgcggcc gcaccatgga ggtggtgaat
gaaatagtct 660 caattgggca ggaagtttta cccaaagttg attatgccca
actctggagt gatgccagtc 720 actgtgaggt gctttacttg tccatcgcat
ttgtcatctt gaagttcact cttggccccc 780 ttggtccaaa aggtcagtct
cgtatgaagt ttgttttcac caattacaac cttctcatgt 840 ccatttattc
gttgggatca ttcctctcaa tggcatatgc catgtacacc atcggtgtta 900
tgtctgacaa ctgcgagaag gcttttgaca acaacgtctt caggatcacc acgcagttgt
960 tctatttgag caagttcctg gagtatattg actccttcta tttgccactg
atgggcaagc 1020 ctctgacctg gttgcaattc ttccatcatt tgggggcacc
gatggatatg tggctgttct 1080 ataattaccg aaatgaagct gtttggattt
ttgtgctgtt gaatggtttc atccactgga 1140 tcatgtacgg ttattattgg
accagattga tcaagctgaa gttccccatg ccaaaatccc 1200 tgattacatc
aatgcagatc attcaattca atgttggttt ctacattgtc tggaagtaca 1260
ggaacattcc ctgttatcgc caagatggga tgaggatgtt tggctggttc ttcaattact
1320 tttatgttgg cacagtcttg tgtttgttct tgaatttcta tgtgcaaacg
tatatcgtca 1380 ggaagcacaa gggagccaaa aagattcagt gagcggccgc
aagtatgaac taaaatgcat 1440 gtaggtgtaa gagctcatgg agagcatgga
atattgtatc cgaccatgta acagtataat 1500 aactgagctc catctcactt
cttctatgaa taaacaaagg atgttatgat atattaacac 1560 tctatctatg
caccttattg ttctatgata aatttcctct tattattata aatcatctga 1620
atcgtgacgg cttatggaat gcttcaaata gtacaaaaac aaatgtgtac tataagactt
1680 tctaaacaat tctaacctta gcattgtgaa cgagacataa gtgttaagaa
gacataacaa 1740 ttataatgga agaagtttgt ctccatttat atattatata
ttacccactt atgtattata 1800 ttaggatgtt aaggagacat aacaattata
aagagagaag tttgtatcca tttatatatt 1860 atatactacc catttatata
ttatacttat ccacttattt aatgtcttta taaggtttga 1920 tccatgatat
ttctaatatt ttagttgata tgtatatgaa agggtactat ttgaactctc 1980
ttactctgta taaaggttgg atcatcctta aagtgggtct atttaatttt attgcttctt
2040 acagataaaa aaaaaattat gagttggttt gataaaatat tgaaggattt
aaaataataa 2100 taaataacat ataatatatg tatataaatt tattataata
taacatttat ctataaaaaa 2160 gtaaatattg tcataaatct atacaatcgt
ttagccttgc tggacgaatc tcaattattt 2220 aaacgagagt aaacatattt
gactttttgg ttatttaaca aattattatt taacactata 2280 tgaaattttt
ttttttatca gcaaagaata aaattaaatt aagaaggaca atggtgtccc 2340
aatccttata caaccaactt ccacaagaaa gtcaagtcag agacaacaaa aaaacaagca
2400 aaggaaattt tttaatttga gttgtcttgt ttgctgcata atttatgcag
taaaacacta 2460 cacataaccc ttttagcagt agagcaatgg ttgaccgtgt
gcttagcttc ttttatttta 2520 tttttttatc agcaaagaat aaataaaata
aaatgagaca cttcagggat gtttcaacaa 2580 gcttggcgcg ccgttctata
gtgtcaccta aatcgtatgt gtatgataca taaggttatg 2640 tattaattgt
agccgcgttc taacgacaat atgtccatat ggtgcactct cagtacaatc 2700
tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
2760 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
ctccgggagc 2820 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
cgagacgaaa gggcctcgtg 2880 atacgcctat ttttataggt taatgtcatg
accaaaatcc cttaacgtga gttttcgttc 2940 cactgagcgt cagaccccgt
agaaaagatc aaaggatctt cttgagatcc tttttttctg 3000 cgcgtaatct
gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 3060
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
3120 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
tgtagcaccg 3180 cctacatacc tcgctctgct aatcctgtta ccagtggctg
ctgccagtgg cgataagtcg 3240 tgtcttaccg ggttggactc aagacgatag
ttaccggata aggcgcagcg gtcgggctga 3300 acggggggtt cgtgcacaca
gcccagcttg gagcgaacga cctacaccga actgagatac 3360
ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
3420 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
gggaaacgcc 3480 tggtatcttt atagtcctgt cgggtttcgc cacctctgac
ttgagcgtcg atttttgtga 3540 tgctcgtcag gggggcggag cctatggaaa
aacgccagca acgcggcctt tttacggttc 3600 ctggcctttt gctggccttt
tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3660 gataaccgta
ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 3720
cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
3780 gcgcgttggc cgattcatta atgcaggttg atcagatctc gatcccgcga
aattaatacg 3840 actcactata gggagaccac aacggtttcc ctctagaaat
aattttgttt aactttaaga 3900 aggagatata cccatggaaa agcctgaact
caccgcgacg tctgtcgaga agtttctgat 3960 cgaaaagttc gacagcgtct
ccgacctgat gcagctctcg gagggcgaag aatctcgtgc 4020 tttcagcttc
gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg 4080
tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
4140 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
gccgtgcaca 4200 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
gctgttctgc agccggtcgc 4260 ggaggctatg gatgcgatcg ctgcggccga
tcttagccag acgagcgggt tcggcccatt 4320 cggaccgcaa ggaatcggtc
aatacactac atggcgtgat ttcatatgcg cgattgctga 4380 tccccatgtg
tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 4440
ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
4500 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
cggtcattga 4560 ctggagcgag gcgatgttcg gggattccca atacgaggtc
gccaacatct tcttctggag 4620 gccgtggttg gcttgtatgg agcagcagac
gcgctacttc gagcggaggc atccggagct 4680 tgcaggatcg ccgcggctcc
gggcgtatat gctccgcatt ggtcttgacc aactctatca 4740 gagcttggtt
gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat 4800
cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
4860 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
ccagcactcg 4920 tccgagggca aaggaatagt gaggtacagc ttggatcgat
ccggctgcta acaaagcccg 4980 aaaggaagct gagttggctg ctgccaccgc
tgagcaataa ctagcataac cccttggggc 5040 ctctaaacgg gtcttgaggg
gttttttgct gaaaggagga actatatccg gatgatcggg 5100 cgcgccgtcg
acggatccgt acgcaaaggc aaagatttaa actcgaaaac attacaaaag 5160
tctcaaaaca gaggcaaggc catgcacaaa gcacactcta agtgcttcca ttgcctacta
5220 agtagggtac gtacacgatc accattcacc agtgatgatc tttattaata
tacaacacac 5280 tcagagacag cttatgttat agctagctag cataaactat
cacatcatgt gttagtacga 5340 caagtgacaa cattgctttt aacttcgcgg
ccttggatcc tctagaccgg atataatgag 5400 ccgtaaacaa agatgattaa
gtagtaatta atacgtacta gtaaaagtgg caaaagataa 5460 cgagaaagaa
ccaatttctt tgcattcggc cttagcggaa ggcatatata agctttgatt 5520
attttattta gtgtaatgat ttcgtacaac caaagcattt atttagtact ctcacacttg
5580 tgtcgcggcc gcgaattcac tagtgattcc ttatagagcc ttccccgcgg
gttgcttctc 5640 cgccatccgg gcgaacaccg ccagatagcg cagcaggatg
accaaccctt catggggcag 5700 cgggttccga tacggcaggt tgtgcttctg
gcacagctgt tccacctggt agctaaccgc 5760 tgtcaggttg tggcgaggga
gggtcggcca caaatggtgc tcaatctggt aattcaagcc 5820 tccgaaaaac
caatctgtga taatccctcg ccgaatgttc atggtctcat ggatctggcc 5880
aaccgagaat ccatggccat cccagactga gtccccgatc ttctccagtg ggtagtggtt
5940 catgaacacc acgatcgcaa tgccgaagcc gccaaccagc tccgaaacga
aaaacaccaa 6000 cagcgatgtg aggatgctgg gcataaagaa taagtggaac
agggtcttca aggtccagtg 6060 cagggcgagg ccaatggcct ccttcttata
ctgagagcga tagaattggt tatctctgtc 6120 cttcaaactg cgcacggtca
acacgctctg gaaacaccaa atgaaccgca acaagataca 6180 gatgaccaag
aaatagtact gctggaactg aatgagcttg cgggaaatcg gtgacgcccg 6240
tgtgacgtca tcctcagacc aggctaagag ggggaggttg tcaatatcag ggtcgtgccc
6300 ttgaacattg gttgccgaat gatgtgcatt gtgtctgtcc ttccaccatg
tcacggaaaa 6360 accttgcaga ccattgccaa ataccagtcc cacgaggttg
ttccagttcc ggttcttgaa 6420 agtctggtgg tggcaaatgt catgagaaag
ccagcccatc tgttgatagt gcatcccaag 6480 caacactgcc ccaatgaaat
acatctgata ctgaaccatc aggaaataac ccagcactcc 6540 aaggcccagt
gtggtgctga ttttgtatga gtaccagagg ggggaggcat caaacatgcc 6600
agttgcgatc aactcttctc ggagcttccg gaaatcctct tgagcttcat tcactgcagc
6660 ctggggtggc aactcagaac tgggattgat tttgggcatg cgcttgagct
tgtcgaaggc 6720 ttcttgagag tgcataacca tgaaggcatc agtggcatcc
cttccttggt aattctctat 6780 aatttccgca ccaccagggt ggaaattgac
ccaggcagac acatcatatg ttgttccatc 6840 aattgtaagg ggaagcgctt
ggcgctttga cttcatttca atcgaattcc cgcggccgct 6900 tggggggcta
tggaagactt tcttagttag ttgtgtgaat aagcaatgtt gggagaatcg 6960
ggactactta taggatagga ataaaacaga aaagtattaa gtgctaatga aatatttaga
7020 ctgataatta aaatcttcac gtatgtccac ttgatataaa aacgtcagga
ataaaggaag 7080 tacagtagaa tttaaaggta ctctttttat atatacccgt
gttctctttt tggctagcta 7140 gttgcataaa aaataatcta tatttttatc
attattttaa atatcttatg agatggtaaa 7200 tatttatcat aatttttttt
actattattt attatttgtg tgtgtaatac atatagaagt 7260 taattacaaa
ttttatttac tttttcatta ttttgatatg attcaccatt aatttagtgt 7320
tattatttat aatagttcat tttaatcttt ttgtatatat tatgcgtgca gtactttttt
7380 cctacatata actactatta cattttattt atataatatt tttattaatg
aattttcgtg 7440 ataatatgta atattgttca ttattatttc agatttttta
aaaatatttg tgttattatt 7500 tatgaaatat gtaatttttt tagtatttga
ttttatgatg ataaagtgtt ctaaattcaa 7560 aagaaggggg aaagcgtaaa
cattaaaaaa cgtcatcaaa caaaaacaaa atcttgttaa 7620 taaagataaa
actgtttgtt ttgatcactg ttatttcgta atataaaaac attatttata 7680
tttatattgt tgacaaccaa atttgcctat caaatctaac caatataatg catgcgtggc
7740 aggtaatgta ctaccatgaa cttaagtcat gacataataa accgtgaatc
tgaccaatgc 7800 atgtacctan ctaaattgta tttgtgacac gaagcaaatg
attcaattca caatggagat 7860 gggaaacaaa taatgaagaa cccagaacta
agaaagcttt tctgaaaaat aaaataaagg 7920 caatgtcaaa agtatactgc
atcatcagtc cagaaagcac atgatatttt tttatcagta 7980 tcaatgcagc
tagttttatt ttacaatatc gatatagcta gtttaaatat attgcagcta 8040
gatttataaa tatttgtgtt attatttatc atttgtgtaa tcctgttttt agtattttag
8100 tttatatatg atgataatgt attccaaatt taaaagaagg gaaataaatt
taaacaagaa 8160 aaaaagtcat caaacaaaaa acaaatgaaa gggtggaaag
atgttaccat gtaatgtgaa 8220 tgttacagta tttcttttat tatagagtta
acaaattaac taatatgatt ttgttaataa 8280 tgataaaata ttttttttat
tattatttca taatataaaa atagtttact taatataaaa 8340 aaaattctat
cgttcacaac aaagttggcc acctaattta accatgcatg tacccatgga 8400
ccatattagg taaccatcaa acctgatgaa gagataaaga gatgaagact taagtcataa
8460 cacaaaacca taaaaaacaa aaatacaatc aaccgtcaat ctgaccaatg
catgaaaaag 8520 ctgcaatagt gagtggcgac acaaagcaca tgattttctt
acaacggaga taaaaccaaa 8580 aaaatatttc atgaacaacc tagaacaaat
aaagctttta tataataaat atataaataa 8640 ataaaggcta tggaataata
tacttcaata tatttggatt aaataaattg ttggcggggt 8700 tgatatattt
atacacacct aaagtcactt caatctcatt ttcacttaac ttttattttt 8760
tttttctttt tatttatcat aaagagaata ttgataatat actttttaac atatttttat
8820 gacatttttt attggtgaaa acttattaaa aatcataaat tttgtaagtt
agatttattt 8880 aaagagttcc tcttcttatt ttaaattttt taataaattt
ttaaataact aaaatttgtg 8940 ttaaaaatgt taaaaaatgt gttattaacc
cttctcttcg aggacgtacg agatccggcc 9000 ggccagatcc tgcaggtctc
aatagattaa gaagttggcg tctcattgat tgaccatggg 9060 ggatcctcta
gacgtacgtg aaggttaaac atggtgaata tgttaccact agctgggatg 9120
cccattagat caaaactgta aaattctccc gtttcccttc tattcacatg tgagccccct
9180 cccttttctt tctttctcaa ttttgattga gttaaagtca ccagcaatgc
atcactcacc 9240 ctccaaaaaa tttcttgtac aacttctcgg actatcccaa
agctcctttt cctgagatgg 9300 atggtcctgt ctcttgccct tgatgtcttc
cttgttcgat tttggcttcc tctaatgtct 9360 ttcttgctag gaatcaccac
ctcactcatc tatgttgtcg tagcttctga aagtctcata 9420 catatcctta
gtgttgcact catcttgtat tgaagtgaaa aagaatgttg ttctcctatc 9480
caaatctcca ttgaatctct ttctcccaat gttgtcccat cggttggtcc tcctctccaa
9540 ccaattgtaa ggtgtttaac ataaacatgg tacaattaag atttttcatt
tcattaagaa 9600 aagattgaga tttgtggttc taaagtttca attagagttt
gatgatattg aaacaaccgt 9660 agaacacatt aagtattact aacttataca
tagagcattg gaatttcacc ttttatttat 9720 tctgtttccg ccaaaggtac
atgactcaag ttattttaca caagtaacaa aggcatctaa 9780 gcctaagtat
tcttattcag acttttcatt attactttca ttgatttggt gcgaaatgcg 9840
gccgcctact cttccttggg acggagtcca agaacacgca agtgctccaa atgtgaagca
9900 aatgcttgcc aaaacgtatc cttgacaagg tatggaacct tgtactcgct
gcaggtgttc 9960 ttgatgatgg ccagaatatc gggataatgg tgctgcgaca
cgttggggaa cagatggtgc 10020 acagcctggt agttcaagct gccagtgatg
ctggtccaga ggtgcgaatc gtgtgcgtaa 10080 tcctgcgtag tctcgacctg
catagctgcc cagtcctttt ggatgatccc gttctcgtca 10140 ggcaacggcc
actgaacttc ctcaacaacg tggttcgcct ggaaggtcag cgccagccag 10200
taagacgaca ccatgtccgc gaccgtgaac aagagcagca ccttgcccag gggcagatac
10260 tgcaggggaa caatcaggcg ataccagaca aagaaagcct tgccgcccca
gaacatcaca 10320 gtgtgccatg tcgagatggg attgacacga atagcgtcat
tggtcttgac aaagtacaaa 10380 atgttgatgt cctgaatgcg caccttgaac
gccagcagtc cgtacaggaa aggaacaaac 10440 atgtgctggt tgatgtggtt
gacaaaccac ttttggttgg gcttgatacg acgaacatcg 10500 ggctcagacg
tcgacacgtc gggatctgct ccagcaatgt tggtgtaggg gtgatggccg 10560
agcatatgtt ggtacatcca caccaggtac gatgctccgt tgaaaaagtc gtgcgtggct
10620 cccagaatct tccagacagt ggggttgtgg gtcactgaaa agtgagacgc
atcatgaaga 10680 gggttgagtc cgacttgtgc gcacgcaaat cccatgatga
ttgcaaacac cacctgaagc 10740 catgtgcgtt cgacaacgaa aggcacaaag
agctgcgcgt agtaggaagc gatcaaggat 10800 ccaaagataa gagcgtatcg
tccccagatc tctggtctat tcttgggatc aatgttccga 10860 tccgtaaagt
agccctcgac tctcgtcttg atggttttgt ggaacaccgt tggctccggg 10920
aagatgggca gctcattcga gaccagtgta ccgacatagt acttcttcat aatggcatct
10980 gcagccccaa acgcgtgata catctcaaag accggagtaa catctcggcc
agctccgagc 11040 aggagagtgt ccactccacc aggatggcgg ctcaagaact
ttgtgacatc gtacaccctg 11100 ccgcggatgg ccaagagtag gtcgtccttg
gtgttatggg ccgccagctc ttcccaggtg 11160 aaggtttttc cttggtccgt
tcccatgcgg ccgcggtgat gactgatgag tgtttaagga 11220 ccaatggaga
gaatgtttga gttgtgaagc ggagaacctg aggcgtggtt atttataggg 11280
aagagaggaa ggtgaatgag ggacacgtca cagaagtagg gtgctgagct tgagacattc
11340 ttcagtatgc atggctatgg aagccttggg tgctacacct catgaagttc
atggtgtgag 11400 gtggcttcgg catctcaatt aagtgacaaa gagaaaggtg
tttcagtgtt tctattgcaa 11460 atggcagaaa ctcgtgatga cgaggggacc
atgcatggtt tcatttcttt tcttcctgga 11520 ttctttcttt ccttttatat
atgcaggttc ataatttaaa aattagactc gctttcaatt 11580 tcttaatttc
tcattttcct cttatattac tgtactaatg ttaaccacgt acacttattt 11640
tttttttagt ttaattttga tagattgtgt tgatttaaac atattaatat tttcaaccaa
11700 ataaaaatca ttttagtaga tacggctttt taaataatta ttaaaaatat
taactattta 11760 tcctaaatgg cacattttaa ttaaaaaaaa tccggtgttg
taagtgtttt attaatttgt 11820 tttggcatta ttaaagcaac ttttttttta
tttgttggca ttttgagtac gtacttaggc 11880 tagcctgca 11889 <210>
SEQ ID NO 90 <211> LENGTH: 5661 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR974 <400> SEQUENCE:
90 gtacgtctag aggatccccc atggtcaatc aatgagacgc caacttctta
atctattgag 60 acctgcaggt ctagaagggc ggatccccgg gtaccgagct
cgaattcact ggccgtcgtt 120 ttacaacgtc gtgactggga aaaccctggc
gttacccaac ttaatcgcct tgcagcacat 180 ccccctttcg ccagctggcg
taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 240 ttgcgcagcc
tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc 300
ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
360 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
tctgctcccg 420 gcatccgctt acagacaagc tgtgaccgtc tccgggagct
gcatgtgtca gaggttttca 480 ccgtcatcac cgaaacgcgc gagacgaaag
ggcctcgtga tacgcctatt tttataggtt 540 aatgtcatga taataatggt
ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 600 ggaaccccta
tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 660
taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
720 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
tcacccagaa 780 acgctggtga aagtaaaaga tgctgaagat cagttgggtg
cacgagtggg ttacatcgaa 840 ctggatctca acagcggtaa gatccttgag
agttttcgcc ccgaagaacg ttttccaatg 900 atgagcactt ttaaagttct
gctatgtggc gcggtattat cccgtattga cgccgggcaa 960 gagcaactcg
gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 1020
acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
1080 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
gaaggagcta 1140 accgcttttt tgcacaacat gggggatcat gtaactcgcc
ttgatcgttg ggaaccggag 1200 ctgaatgaag ccataccaaa cgacgagcgt
gacaccacga tgcctgtagc aatggcaaca 1260 acgttgcgca aactattaac
tggcgaacta cttactctag cttcccggca acaattaata 1320 gactggatgg
aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 1380
tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
1440 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
gagtcaggca 1500 actatggatg aacgaaatag acagatcgct gagataggtg
cctcactgat taagcattgg 1560 taactgtcag accaagttta ctcatatata
ctttagattg atttaaaact tcatttttaa 1620 tttaaaagga tctaggtgaa
gatccttttt gataatctca tgaccaaaat cccttaacgt 1680 gagttttcgt
tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 1740
cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
1800 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
cttcagcaga 1860 gcgcagatac caaatactgt ccttctagtg tagccgtagt
taggccacca cttcaagaac 1920 tctgtagcac cgcctacata cctcgctctg
ctaatcctgt taccagtggc tgctgccagt 1980 ggcgataagt cgtgtcttac
cgggttggac tcaagacgat agttaccgga taaggcgcag 2040 cggtcgggct
gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2100
gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
2160 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
ggagcttcca 2220 gggggaaacg cctggtatct ttatagtcct gtcgggtttc
gccacctctg acttgagcgt 2280 cgatttttgt gatgctcgtc aggggggcgg
agcctatgga aaaacgccag caacgcggcc 2340 tttttacggt tcctggcctt
ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 2400 cctgattctg
tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 2460
cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
2520 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
gtttcccgac 2580 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
agctcactca ttaggcaccc 2640 caggctttac actttatgct tccggctcgt
atgttgtgtg gaattgtgag cggataacaa 2700 tttcacacag gaaacagcta
tgaccatgat tacgccaagc ttgcatgcct gcaggctagc 2760 ctaagtacgt
actcaaaatg ccaacaaata aaaaaaaagt tgctttaata atgccaaaac 2820
aaattaataa aacacttaca acaccggatt ttttttaatt aaaatgtgcc atttaggata
2880 aatagttaat atttttaata attatttaaa aagccgtatc tactaaaatg
atttttattt 2940 ggttgaaaat attaatatgt ttaaatcaac acaatctatc
aaaattaaac taaaaaaaaa 3000 ataagtgtac gtggttaaca ttagtacagt
aatataagag gaaaatgaga aattaagaaa 3060 ttgaaagcga gtctaatttt
taaattatga acctgcatat ataaaaggaa agaaagaatc 3120 caggaagaaa
agaaatgaaa ccatgcatgg tcccctcgtc atcacgagtt tctgccattt 3180
gcaatagaaa cactgaaaca cctttctctt tgtcacttaa ttgagatgcc gaagccacct
3240 cacaccatga acttcatgag gtgtagcacc caaggcttcc atagccatgc
atactgaaga 3300 atgtctcaag ctcagcaccc tacttctgtg acgtgtccct
cattcacctt cctctcttcc 3360 ctataaataa ccacgcctca ggttctccgc
ttcacaactc aaacattctc tccattggtc 3420 cttaaacact catcagtcat
caccgcggcc gccaattcat ggccccgcag acggagctcc 3480 gccagcgcca
cgccgccgtc gccgagacgc cggtggccgg caagaaggcc tttacatggc 3540
aggaggtcgc gcagcacaac acggcggcct cggcctggat cattatccgc ggcaaggtct
3600 acgacgtgac cgagtgggcc aacaagcacc ccggcggccg cgagatggtg
ctgctgcacg 3660 ccggtcgcga ggccaccgac acgttcgact cgtaccaccc
gttcagcgac aaggccgagt 3720 cgatcttgaa caagtatgag attggcacgt
tcacgggccc gtccgagttt ccgaccttca 3780 agccggacac gggcttctac
aaggagtgcc gcaagcgcgt tggcgagtac ttcaagaaga 3840 acaacctcca
tccgcaggac ggcttcccgg gcctctggcg catgatggtc gtgtttgcgg 3900
tcgccggcct cgccttgtac ggcatgcact tttcgactat ctttgcgctg cagctcgcgg
3960 ccgcggcgct ctttggcgtc tgccaggcgc tgccgctgct ccacgtcatg
cacgactcgt 4020 cgcacgcgtc gtacaccaac atgccgttct tccattacgt
cgtcggccgc tttgccatgg 4080 actggtttgc cggcggctcg atggtgtcat
ggctcaacca gcacgtcgtg ggccaccaca 4140 tctacacgaa cgtcgcgggc
tcggacccgg atcttccggt caacatggac ggcgacatcc 4200 gccgcatcgt
gaaccgccag gtgttccagc ccatgtacgc attccagcac atctaccttc 4260
cgccgctcta tggcgtgctt ggcctcaagt tccgcatcca ggacttcacc gacacgttcg
4320 gctcgcacac gaacggcccg atccgcgtca acccgcacgc gctctcgacg
tggatggcca 4380 tgatcagctc caagtcgttc tgggccttct accgcgtgta
ccttccgctt gccgtgctcc 4440 agatgcccat caagacgtac cttgcgatct
tcttcctcgc cgagtttgtc acgggctggt 4500 acctcgcgtt caacttccaa
gtaagccatg tctcgaccga gtgcggctac ccatgcggcg 4560 acgaggccaa
gatggcgctc caggacgagt gggcagtctc gcaggtcaag acgtcggtcg 4620
actacgccca tggctcgtgg atgacgacgt tccttgccgg cgcgctcaac taccaggtcg
4680 tgcaccactt gttccccagc gtgtcgcagt accactaccc ggcgatcgcg
cccatcatcg 4740 tcgacgtctg caaggagtac aacatcaagt acgccatctt
gccggacttt acggcggcgt 4800 tcgttgccca cttgaagcac ctccgcaaca
tgggccagca gggcatcgcc gccacgatcc 4860 acatgggcta actcgagctc
agctagatcg cggccgcatt tcgcaccaaa tcaatgaaag 4920 taataatgaa
aagtctgaat aagaatactt aggcttagat gcctttgtta cttgtgtaaa 4980
ataacttgag tcatgtacct ttggcggaaa cagaataaat aaaaggtgaa attccaatgc
5040 tctatgtata agttagtaat acttaatgtg ttctacggtt gtttcaatat
catcaaactc 5100 taattgaaac tttagaacca caaatctcaa tcttttctta
atgaaatgaa aaatcttaat 5160 tgtaccatgt ttatgttaaa caccttacaa
ttaattggtt ggagaggagg accaaccgat 5220 gggacaacat tgggagaaag
agattcaatg gagatttgga taggagaaca acattctttt 5280 tcacttcaat
acaagatgag tgcaacacta aggatatgta tgagactttc agaagctacg 5340
acaacataga tgagtgaggt ggtgattcct agcaagaaag acattagagg aagccaaaat
5400 cgaacaagga agacatcaag ggcaagagac aggaccatcc atctcaggaa
aaggagcttt 5460 gggatagtcc gagaagttgt acaagaaatt ttttggaggg
tgagtgatgc attgctggtg 5520 actttaactc aatcaaaatt gagaaagaaa
gaaaagggag ggggctcaca tgtgaataga 5580 agggaaacgg gagaatttta
cagttttgat ctaatgggca tcccagctag tggtaacata 5640 ttcaccatgt
ttaaccttca c 5661 <210> SEQ ID NO 91 <211> LENGTH: 5578
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid pKR1032
<400> SEQUENCE: 91 ggccgcattt cgcaccaaat caatgaaagt
aataatgaaa agtctgaata agaatactta 60 ggcttagatg cctttgttac
ttgtgtaaaa taacttgagt catgtacctt tggcggaaac 120 agaataaata
aaaggtgaaa ttccaatgct ctatgtataa gttagtaata cttaatgtgt 180
tctacggttg tttcaatatc atcaaactct aattgaaact ttagaaccac aaatctcaat
240 cttttcttaa tgaaatgaaa aatcttaatt gtaccatgtt tatgttaaac
accttacaat 300 taattggttg gagaggagga ccaaccgatg ggacaacatt
gggagaaaga gattcaatgg 360 agatttggat aggagaacaa cattcttttt
cacttcaata caagatgagt gcaacactaa 420 ggatatgtat gagactttca
gaagctacga caacatagat gagtgaggtg gtgattccta 480 gcaagaaaga
cattagagga agccaaaatc gaacaaggaa gacatcaagg gcaagagaca 540
ggaccatcca tctcaggaaa aggagctttg ggatagtccg agaagttgta caagaaattt
600 tttggagggt gagtgatgca ttgctggtga ctttaactca atcaaaattg
agaaagaaag 660 aaaagggagg gggctcacat gtgaatagaa gggaaacggg
agaattttac agttttgatc 720 taatgggcat cccagctagt ggtaacatat
tcaccatgtt taaccttcac gtacgtctag 780 aggatccccc atggtcaatc
aatgagacgc caacttctta atctattgag acctgcaggt 840 ctagaagggc
ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc 900
gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
960 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
ttgcgcagcc 1020 tgaatggcga atggcgcctg atgcggtatt ttctccttac
gcatctgtgc ggtatttcac 1080 accgcatatg gtgcactctc agtacaatct
gctctgatgc cgcatagtta agccagcccc 1140 gacacccgcc aacacccgct
gacgcgccct gacgggcttg tctgctcccg gcatccgctt 1200 acagacaagc
tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 1260
cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
1320 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
ggaaccccta 1380 tttgtttatt tttctaaata cattcaaata tgtatccgct
catgagacaa taaccctgat 1440 aaatgcttca ataatattga aaaaggaaga
gtatgagtat tcaacatttc cgtgtcgccc 1500 ttattccctt ttttgcggca
ttttgccttc ctgtttttgc tcacccagaa acgctggtga 1560 aagtaaaaga
tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 1620
acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
1680 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
gagcaactcg 1740 gtcgccgcat acactattct cagaatgact tggttgagta
ctcaccagtc acagaaaagc 1800 atcttacgga tggcatgaca gtaagagaat
tatgcagtgc tgccataacc atgagtgata 1860 acactgcggc caacttactt
ctgacaacga tcggaggacc gaaggagcta accgcttttt 1920 tgcacaacat
gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 1980
ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
2040 aactattaac tggcgaacta cttactctag cttcccggca acaattaata
gactggatgg 2100 aggcggataa agttgcagga ccacttctgc gctcggccct
tccggctggc tggtttattg 2160 ctgataaatc tggagccggt gagcgtgggt
ctcgcggtat cattgcagca ctggggccag 2220 atggtaagcc ctcccgtatc
gtagttatct acacgacggg gagtcaggca actatggatg 2280 aacgaaatag
acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 2340
accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
2400 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
gagttttcgt 2460 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
ttcttgagat cctttttttc 2520 tgcgcgtaat ctgctgcttg caaacaaaaa
aaccaccgct accagcggtg gtttgtttgc 2580 cggatcaaga gctaccaact
ctttttccga aggtaactgg cttcagcaga gcgcagatac 2640 caaatactgt
ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2700
cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
2760 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
cggtcgggct 2820 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
gacctacacc gaactgagat 2880 acctacagcg tgagctatga gaaagcgcca
cgcttcccga agggagaaag gcggacaggt 2940 atccggtaag cggcagggtc
ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3000 cctggtatct
ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3060
gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
3120 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
cctgattctg 3180 tggataaccg tattaccgcc tttgagtgag ctgataccgc
tcgccgcagc cgaacgaccg 3240 agcgcagcga gtcagtgagc gaggaagcgg
aagagcgccc aatacgcaaa ccgcctctcc 3300 ccgcgcgttg gccgattcat
taatgcagct ggcacgacag gtttcccgac tggaaagcgg 3360 gcagtgagcg
caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 3420
actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
3480 gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggctagc
ctaagtacgt 3540 actcaaaatg ccaacaaata aaaaaaaagt tgctttaata
atgccaaaac aaattaataa 3600 aacacttaca acaccggatt ttttttaatt
aaaatgtgcc atttaggata aatagttaat 3660 atttttaata attatttaaa
aagccgtatc tactaaaatg atttttattt ggttgaaaat 3720 attaatatgt
ttaaatcaac acaatctatc aaaattaaac taaaaaaaaa ataagtgtac 3780
gtggttaaca ttagtacagt aatataagag gaaaatgaga aattaagaaa ttgaaagcga
3840 gtctaatttt taaattatga acctgcatat ataaaaggaa agaaagaatc
caggaagaaa 3900 agaaatgaaa ccatgcatgg tcccctcgtc atcacgagtt
tctgccattt gcaatagaaa 3960 cactgaaaca cctttctctt tgtcacttaa
ttgagatgcc gaagccacct cacaccatga 4020 acttcatgag gtgtagcacc
caaggcttcc atagccatgc atactgaaga atgtctcaag 4080 ctcagcaccc
tacttctgtg acgtgtccct cattcacctt cctctcttcc ctataaataa 4140
ccacgcctca ggttctccgc ttcacaactc aaacattctc tccattggtc cttaaacact
4200 catcagtcat caccgcggcc gcaaaccatg gctctcagtc ttaccacaga
acagctgtta 4260 gaacgccctg atttggttgc gattgatggc atcctctacg
accttgaagg gcttgccaaa 4320 gttcatccag gaggagattt gattctcgct
tctggtgcct ctgatgcctc ccctctcttt 4380 tattcaatgc atccatacgt
caaaccggag aattccaaat tgcttcaaca gttcgtccga 4440 gggaagcatg
accgcacctc gaaggacatt gtctacacgt atgattctcc cttcgcacaa 4500
gacgttaagc ggacaatgcg cgaggtgatg aaagggagga actggtacgc aacccctggc
4560 ttctggctgc gcaccgttgg gatcatcgcc gtgacggcct tttgcgagtg
gcactgggct 4620 accacgggga tggtgctgtg gggcctgttg actggattca
tgcacatgca gatcggctta 4680 tccatccagc atgatgcgtc ccacggggcc
atcagcaaga agccttgggt caacgccctc 4740 ttcgcctacg gcattgacgt
catcggatcg tcccggtgga tttggctgca gtcgcacatc 4800 atgcggcacc
acacctacac caaccagcac ggcctcgacc tggatgcgga gtcggcagag 4860
ccgttcctgg tgttccacaa ctaccccgcc gcaaacaccg cccgaaagtg gttccaccgc
4920 ttccaagctt ggtacatgta ccttgtgctg ggggcatacg gggtatcgct
ggtgtacaac 4980 ccgctctaca ttttccggat gcagcacaat gacaccatcc
cagagtctgt cacggccatg 5040 cgggaaaatg gctttctgcg gcgctaccgc
acacttgcat tcgtgatgcg agctttcttc 5100 atcttccgga ccgcattctt
gccctggtac ctcactggga cctcattgct gatcaccatt 5160 cctctggtgc
ccaccgcaac tggtgccttc ttgacgttct tcttcatttt gtcccacaat 5220
tttgatggct ccgaacggat ccccgacaag aactgcaagg ttaagagatc tgagaaggac
5280 gttgaggctg accaaattga ctggtatcgg gcgcaggtgg agacgtcctc
cacatacggt 5340 ggccccatcg ccatgttctt cactggcggt ctcaatttcc
agatcgagca ccacctcttt 5400 ccccggatgt cgtcttggca ctaccccttc
gtccagcagg cggtccggga gtgttgcgaa 5460 cgccatggag tgcgatatgt
tttctaccct accatcgtcg gcaacatcat ctccaccctg 5520 aagtacatgc
ataaggtggg tgtcgtccac tgcgtgaagg acgcacagga ttcctaag 5578
<210> SEQ ID NO 92 <211> LENGTH: 11907 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pKR1037 <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(7810)..(7810) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 92 ggagatccaa gcttttgatc catgcccttc
atttgccgct tattaattaa tttggtaaca 60 gtccgtacta atcagttact
tatccttccc ccatcataat taatcttggt agtctcgaat 120 gccacaacac
tgactagtct cttggatcat aagaaaaagc caaggaacaa aagaagacaa 180
aacacaatga gagtatcctt tgcatagcaa tgtctaagtt cataaaattc aaacaaaaac
240 gcaatcacac acagtggaca tcacttatcc actagctgat caggatcgcc
gcgtcaagaa 300 aaaaaaactg gaccccaaaa gccatgcaca acaacacgta
ctcacaaagg tgtcaatcga 360 gcagcccaaa acattcacca actcaaccca
tcatgagccc tcacatttgt tgtttctaac 420 ccaacctcaa actcgtattc
tcttccgcca cctcattttt gtttatttca acacccgtca 480 aactgcatgc
caccccgtgg ccaaatgtcc atgcatgtta acaagaccta tgactataaa 540
tagctgcaat ctcggcccag gttttcatca tcaagaacca gttcaatatc ctagtacacc
600 gtattaaaga atttaagata tactgcggcc gcaccatgga ggtggtgaat
gaaatagtct 660 caattgggca ggaagtttta cccaaagttg attatgccca
actctggagt gatgccagtc 720 actgtgaggt gctttacttg tccatcgcat
ttgtcatctt gaagttcact cttggccccc 780 ttggtccaaa aggtcagtct
cgtatgaagt ttgttttcac caattacaac cttctcatgt 840 ccatttattc
gttgggatca ttcctctcaa tggcatatgc catgtacacc atcggtgtta 900
tgtctgacaa ctgcgagaag gcttttgaca acaacgtctt caggatcacc acgcagttgt
960 tctatttgag caagttcctg gagtatattg actccttcta tttgccactg
atgggcaagc 1020 ctctgacctg gttgcaattc ttccatcatt tgggggcacc
gatggatatg tggctgttct 1080 ataattaccg aaatgaagct gtttggattt
ttgtgctgtt gaatggtttc atccactgga 1140 tcatgtacgg ttattattgg
accagattga tcaagctgaa gttccccatg ccaaaatccc 1200 tgattacatc
aatgcagatc attcaattca atgttggttt ctacattgtc tggaagtaca 1260
ggaacattcc ctgttatcgc caagatggga tgaggatgtt tggctggttc ttcaattact
1320 tttatgttgg cacagtcttg tgtttgttct tgaatttcta tgtgcaaacg
tatatcgtca 1380 ggaagcacaa gggagccaaa aagattcagt gagcggccgc
aagtatgaac taaaatgcat 1440 gtaggtgtaa gagctcatgg agagcatgga
atattgtatc cgaccatgta acagtataat 1500 aactgagctc catctcactt
cttctatgaa taaacaaagg atgttatgat atattaacac 1560 tctatctatg
caccttattg ttctatgata aatttcctct tattattata aatcatctga 1620
atcgtgacgg cttatggaat gcttcaaata gtacaaaaac aaatgtgtac tataagactt
1680 tctaaacaat tctaacctta gcattgtgaa cgagacataa gtgttaagaa
gacataacaa 1740
ttataatgga agaagtttgt ctccatttat atattatata ttacccactt atgtattata
1800 ttaggatgtt aaggagacat aacaattata aagagagaag tttgtatcca
tttatatatt 1860 atatactacc catttatata ttatacttat ccacttattt
aatgtcttta taaggtttga 1920 tccatgatat ttctaatatt ttagttgata
tgtatatgaa agggtactat ttgaactctc 1980 ttactctgta taaaggttgg
atcatcctta aagtgggtct atttaatttt attgcttctt 2040 acagataaaa
aaaaaattat gagttggttt gataaaatat tgaaggattt aaaataataa 2100
taaataacat ataatatatg tatataaatt tattataata taacatttat ctataaaaaa
2160 gtaaatattg tcataaatct atacaatcgt ttagccttgc tggacgaatc
tcaattattt 2220 aaacgagagt aaacatattt gactttttgg ttatttaaca
aattattatt taacactata 2280 tgaaattttt ttttttatca gcaaagaata
aaattaaatt aagaaggaca atggtgtccc 2340 aatccttata caaccaactt
ccacaagaaa gtcaagtcag agacaacaaa aaaacaagca 2400 aaggaaattt
tttaatttga gttgtcttgt ttgctgcata atttatgcag taaaacacta 2460
cacataaccc ttttagcagt agagcaatgg ttgaccgtgt gcttagcttc ttttatttta
2520 tttttttatc agcaaagaat aaataaaata aaatgagaca cttcagggat
gtttcaacaa 2580 gcttggcgcg ccgttctata gtgtcaccta aatcgtatgt
gtatgataca taaggttatg 2640 tattaattgt agccgcgttc taacgacaat
atgtccatat ggtgcactct cagtacaatc 2700 tgctctgatg ccgcatagtt
aagccagccc cgacacccgc caacacccgc tgacgcgccc 2760 tgacgggctt
gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 2820
tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
2880 atacgcctat ttttataggt taatgtcatg accaaaatcc cttaacgtga
gttttcgttc 2940 cactgagcgt cagaccccgt agaaaagatc aaaggatctt
cttgagatcc tttttttctg 3000 cgcgtaatct gctgcttgca aacaaaaaaa
ccaccgctac cagcggtggt ttgtttgccg 3060 gatcaagagc taccaactct
ttttccgaag gtaactggct tcagcagagc gcagatacca 3120 aatactgtcc
ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 3180
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
3240 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
gtcgggctga 3300 acggggggtt cgtgcacaca gcccagcttg gagcgaacga
cctacaccga actgagatac 3360 ctacagcgtg agcattgaga aagcgccacg
cttcccgaag ggagaaaggc ggacaggtat 3420 ccggtaagcg gcagggtcgg
aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3480 tggtatcttt
atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3540
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
3600 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
tgattctgtg 3660 gataaccgta ttaccgcctt tgagtgagct gataccgctc
gccgcagccg aacgaccgag 3720 cgcagcgagt cagtgagcga ggaagcggaa
gagcgcccaa tacgcaaacc gcctctcccc 3780 gcgcgttggc cgattcatta
atgcaggttg atcagatctc gatcccgcga aattaatacg 3840 actcactata
gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga 3900
aggagatata cccatggaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
3960 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
aatctcgtgc 4020 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
gtaaatagct gcgccgatgg 4080 tttctacaaa gatcgttatg tttatcggca
ctttgcatcg gccgcgctcc cgattccgga 4140 agtgcttgac attggggaat
tcagcgagag cctgacctat tgcatctccc gccgtgcaca 4200 gggtgtcacg
ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc 4260
ggaggctatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
4320 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
cgattgctga 4380 tccccatgtg tatcactggc aaactgtgat ggacgacacc
gtcagtgcgt ccgtcgcgca 4440 ggctctcgat gagctgatgc tttgggccga
ggactgcccc gaagtccggc acctcgtgca 4500 cgcggatttc ggctccaaca
atgtcctgac ggacaatggc cgcataacag cggtcattga 4560 ctggagcgag
gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag 4620
gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
4680 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
aactctatca 4740 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
cagggtcgat gcgacgcaat 4800 cgtccgatcc ggagccggga ctgtcgggcg
tacacaaatc gcccgcagaa gcgcggccgt 4860 ctggaccgat ggctgtgtag
aagtactcgc cgatagtgga aaccgacgcc ccagcactcg 4920 tccgagggca
aaggaatagt gaggtacagc ttggatcgat ccggctgcta acaaagcccg 4980
aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
5040 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
gatgatcggg 5100 cgcgccgtcg acggatccgt acgcaaaggc aaagatttaa
actcgaaaac attacaaaag 5160 tctcaaaaca gaggcaaggc catgcacaaa
gcacactcta agtgcttcca ttgcctacta 5220 agtagggtac gtacacgatc
accattcacc agtgatgatc tttattaata tacaacacac 5280 tcagagacag
cttatgttat agctagctag cataaactat cacatcatgt gttagtacga 5340
caagtgacaa cattgctttt aacttcgcgg ccttggatcc tctagaccgg atataatgag
5400 ccgtaaacaa agatgattaa gtagtaatta atacgtacta gtaaaagtgg
caaaagataa 5460 cgagaaagaa ccaatttctt tgcattcggc cttagcggaa
ggcatatata agctttgatt 5520 attttattta gtgtaatgat ttcgtacaac
caaagcattt atttagtact ctcacacttg 5580 tgtcgcggcc gcgaattcac
tagtgattcc ttatagagcc ttccccgcgg gttgcttctc 5640 cgccatccgg
gcgaacaccg ccagatagcg cagcaggatg accaaccctt catggggcag 5700
cgggttccga tacggcaggt tgtgcttctg gcacagctgt tccacctggt agctaaccgc
5760 tgtcaggttg tggcgaggga gggtcggcca caaatggtgc tcaatctggt
aattcaagcc 5820 tccgaaaaac caatctgtga taatccctcg ccgaatgttc
atggtctcat ggatctggcc 5880 aaccgagaat ccatggccat cccagactga
gtccccgatc ttctccagtg ggtagtggtt 5940 catgaacacc acgatcgcaa
tgccgaagcc gccaaccagc tccgaaacga aaaacaccaa 6000 cagcgatgtg
aggatgctgg gcataaagaa taagtggaac agggtcttca aggtccagtg 6060
cagggcgagg ccaatggcct ccttcttata ctgagagcga tagaattggt tatctctgtc
6120 cttcaaactg cgcacggtca acacgctctg gaaacaccaa atgaaccgca
acaagataca 6180 gatgaccaag aaatagtact gctggaactg aatgagcttg
cgggaaatcg gtgacgcccg 6240 tgtgacgtca tcctcagacc aggctaagag
ggggaggttg tcaatatcag ggtcgtgccc 6300 ttgaacattg gttgccgaat
gatgtgcatt gtgtctgtcc ttccaccatg tcacggaaaa 6360 accttgcaga
ccattgccaa ataccagtcc cacgaggttg ttccagttcc ggttcttgaa 6420
agtctggtgg tggcaaatgt catgagaaag ccagcccatc tgttgatagt gcatcccaag
6480 caacactgcc ccaatgaaat acatctgata ctgaaccatc aggaaataac
ccagcactcc 6540 aaggcccagt gtggtgctga ttttgtatga gtaccagagg
ggggaggcat caaacatgcc 6600 agttgcgatc aactcttctc ggagcttccg
gaaatcctct tgagcttcat tcactgcagc 6660 ctggggtggc aactcagaac
tgggattgat tttgggcatg cgcttgagct tgtcgaaggc 6720 ttcttgagag
tgcataacca tgaaggcatc agtggcatcc cttccttggt aattctctat 6780
aatttccgca ccaccagggt ggaaattgac ccaggcagac acatcatatg ttgttccatc
6840 aattgtaagg ggaagcgctt ggcgctttga cttcatttca atcgaattcc
cgcggccgct 6900 tggggggcta tggaagactt tcttagttag ttgtgtgaat
aagcaatgtt gggagaatcg 6960 ggactactta taggatagga ataaaacaga
aaagtattaa gtgctaatga aatatttaga 7020 ctgataatta aaatcttcac
gtatgtccac ttgatataaa aacgtcagga ataaaggaag 7080 tacagtagaa
tttaaaggta ctctttttat atatacccgt gttctctttt tggctagcta 7140
gttgcataaa aaataatcta tatttttatc attattttaa atatcttatg agatggtaaa
7200 tatttatcat aatttttttt actattattt attatttgtg tgtgtaatac
atatagaagt 7260 taattacaaa ttttatttac tttttcatta ttttgatatg
attcaccatt aatttagtgt 7320 tattatttat aatagttcat tttaatcttt
ttgtatatat tatgcgtgca gtactttttt 7380 cctacatata actactatta
cattttattt atataatatt tttattaatg aattttcgtg 7440 ataatatgta
atattgttca ttattatttc agatttttta aaaatatttg tgttattatt 7500
tatgaaatat gtaatttttt tagtatttga ttttatgatg ataaagtgtt ctaaattcaa
7560 aagaaggggg aaagcgtaaa cattaaaaaa cgtcatcaaa caaaaacaaa
atcttgttaa 7620 taaagataaa actgtttgtt ttgatcactg ttatttcgta
atataaaaac attatttata 7680 tttatattgt tgacaaccaa atttgcctat
caaatctaac caatataatg catgcgtggc 7740 aggtaatgta ctaccatgaa
cttaagtcat gacataataa accgtgaatc tgaccaatgc 7800 atgtacctan
ctaaattgta tttgtgacac gaagcaaatg attcaattca caatggagat 7860
gggaaacaaa taatgaagaa cccagaacta agaaagcttt tctgaaaaat aaaataaagg
7920 caatgtcaaa agtatactgc atcatcagtc cagaaagcac atgatatttt
tttatcagta 7980 tcaatgcagc tagttttatt ttacaatatc gatatagcta
gtttaaatat attgcagcta 8040 gatttataaa tatttgtgtt attatttatc
atttgtgtaa tcctgttttt agtattttag 8100 tttatatatg atgataatgt
attccaaatt taaaagaagg gaaataaatt taaacaagaa 8160 aaaaagtcat
caaacaaaaa acaaatgaaa gggtggaaag atgttaccat gtaatgtgaa 8220
tgttacagta tttcttttat tatagagtta acaaattaac taatatgatt ttgttaataa
8280 tgataaaata ttttttttat tattatttca taatataaaa atagtttact
taatataaaa 8340 aaaattctat cgttcacaac aaagttggcc acctaattta
accatgcatg tacccatgga 8400 ccatattagg taaccatcaa acctgatgaa
gagataaaga gatgaagact taagtcataa 8460 cacaaaacca taaaaaacaa
aaatacaatc aaccgtcaat ctgaccaatg catgaaaaag 8520 ctgcaatagt
gagtggcgac acaaagcaca tgattttctt acaacggaga taaaaccaaa 8580
aaaatatttc atgaacaacc tagaacaaat aaagctttta tataataaat atataaataa
8640 ataaaggcta tggaataata tacttcaata tatttggatt aaataaattg
ttggcggggt 8700 tgatatattt atacacacct aaagtcactt caatctcatt
ttcacttaac ttttattttt 8760 tttttctttt tatttatcat aaagagaata
ttgataatat actttttaac atatttttat 8820 gacatttttt attggtgaaa
acttattaaa aatcataaat tttgtaagtt agatttattt 8880 aaagagttcc
tcttcttatt ttaaattttt taataaattt ttaaataact aaaatttgtg 8940
ttaaaaatgt taaaaaatgt gttattaacc cttctcttcg aggacgtacg agatccggcc
9000 ggccagatcc tgcaggtctc aatagattaa gaagttggcg tctcattgat
tgaccatggg 9060 ggatcctcta gacgtacgtg aaggttaaac atggtgaata
tgttaccact agctgggatg 9120 cccattagat caaaactgta aaattctccc
gtttcccttc tattcacatg tgagccccct 9180 cccttttctt tctttctcaa
ttttgattga gttaaagtca ccagcaatgc atcactcacc 9240
ctccaaaaaa tttcttgtac aacttctcgg actatcccaa agctcctttt cctgagatgg
9300 atggtcctgt ctcttgccct tgatgtcttc cttgttcgat tttggcttcc
tctaatgtct 9360 ttcttgctag gaatcaccac ctcactcatc tatgttgtcg
tagcttctga aagtctcata 9420 catatcctta gtgttgcact catcttgtat
tgaagtgaaa aagaatgttg ttctcctatc 9480 caaatctcca ttgaatctct
ttctcccaat gttgtcccat cggttggtcc tcctctccaa 9540 ccaattaatt
gtaaggtgtt taacataaac atggtacaat taagattttt catttcatta 9600
agaaaagatt gagatttgtg gttctaaagt ttcaattaga gtttgatgat attgaaacaa
9660 ccgtagaaca cattaagtat tactaactta tacatagagc attggaattt
caccttttat 9720 ttattctgtt tccgccaaag gtacatgact caagttattt
tacacaagta acaaaggcat 9780 ctaagcctaa gtattcttat tcagactttt
cattattact ttcattgatt tggtgcgaaa 9840 tgcggccgct taggaatcct
gtgcgtcctt cacgcagtgg acgacaccca ccttatgcat 9900 gtacttcagg
gtggagatga tgttgccgac gatggtaggg tagaaaacat atcgcactcc 9960
atggcgttcg caacactccc ggaccgcctg ctggacgaag gggtagtgcc aagacgacat
10020 ccggggaaag aggtggtgct cgatctggaa attgagaccg ccagtgaaga
acatggcgat 10080 ggggccaccg tatgtggagg acgtctccac ctgcgcccga
taccagtcaa tttggtcagc 10140 ctcaacgtcc ttctcagatc tcttaacctt
gcagttcttg tcggggatcc gttcggagcc 10200 atcaaaattg tgggacaaaa
tgaagaagaa cgtcaagaag gcaccagttg cggtgggcac 10260 cagaggaatg
gtgatcagca atgaggtccc agtgaggtac cagggcaaga atgcggtccg 10320
gaagatgaag aaagctcgca tcacgaatgc aagtgtgcgg tagcgccgca gaaagccatt
10380 ttcccgcatg gccgtgacag actctgggat ggtgtcattg tgctgcatcc
ggaaaatgta 10440 gagcgggttg tacaccagcg ataccccgta tgcccccagc
acaaggtaca tgtaccaagc 10500 ttggaagcgg tggaaccact ttcgggcggt
gtttgcggcg gggtagttgt ggaacaccag 10560 gaacggctct gccgactccg
catccaggtc gaggccgtgc tggttggtgt aggtgtggtg 10620 ccgcatgatg
tgcgactgca gccaaatcca ccgggacgat ccgatgacgt caatgccgta 10680
ggcgaagagg gcgttgaccc aaggcttctt gctgatggcc ccgtgggacg catcatgctg
10740 gatggataag ccgatctgca tgtgcatgaa tccagtcaac aggccccaca
gcaccatccc 10800 cgtggtagcc cagtgccact cgcaaaaggc cgtcacggcg
atgatcccaa cggtgcgcag 10860 ccagaagcca ggggttgcgt accagttcct
ccctttcatc acctcgcgca ttgtccgctt 10920 aacgtcttgt gcgaagggag
aatcatacgt gtagacaatg tccttcgagg tgcggtcatg 10980 cttccctcgg
acgaactgtt gaagcaattt ggaattctcc ggtttgacgt atggatgcat 11040
tgaataaaag agaggggagg catcagaggc accagaagcg agaatcaaat ctcctcctgg
11100 atgaactttg gcaagccctt caaggtcgta gaggatgcca tcaatcgcaa
ccaaatcagg 11160 gcgttctaac agctgttctg tggtaagact gagagccatg
gtttgcggcc gcggtgatga 11220 ctgatgagtg tttaaggacc aatggagaga
atgtttgagt tgtgaagcgg agaacctgag 11280 gcgtggttat ttatagggaa
gagaggaagg tgaatgaggg acacgtcaca gaagtagggt 11340 gctgagcttg
agacattctt cagtatgcat ggctatggaa gccttgggtg ctacacctca 11400
tgaagttcat ggtgtgaggt ggcttcggca tctcaattaa gtgacaaaga gaaaggtgtt
11460 tcagtgtttc tattgcaaat ggcagaaact cgtgatgacg aggggaccat
gcatggtttc 11520 atttcttttc ttcctggatt ctttctttcc ttttatatat
gcaggttcat aatttaaaaa 11580 ttagactcgc tttcaatttc ttaatttctc
attttcctct tatattactg tactaatgtt 11640 aaccacgtac acttattttt
tttttagttt aattttgata gattgtgttg atttaaacat 11700 attaatattt
tcaaccaaat aaaaatcatt ttagtagata cggcttttta aataattatt 11760
aaaaatatta actatttatc ctaaatggca cattttaatt aaaaaaaatc cggtgttgta
11820 agtgttttat taatttgttt tggcattatt aaagcaactt tttttttatt
tgttggcatt 11880 ttgagtacgt acttaggcta gcctgca 11907 <210>
SEQ ID NO 93 <211> LENGTH: 8671 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKR328 <400> SEQUENCE:
93 ggatctggcc ggccggatct cgtacggatc cgtcgacggc gcgcccgatc
atccggatat 60 agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa ggggttatgc 120 tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt tgttagcagc 180 cggatcgatc caagctgtac
ctcactattc ctttgccctc ggacgagtgc tggggcgtcg 240 gtttccacta
tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg 300
ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
360 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
gttggtcaag 420 accaatgcgg agcatatacg cccggagccg cggcgatcct
gcaagctccg gatgcctccg 480 ctcgaagtag cgcgtctgct gctccataca
agccaaccac ggcctccaga agaagatgtt 540 ggcgacctcg tattgggaat
ccccgaacat cgcctcgctc cagtcaatga ccgctgttat 600 gcggccattg
tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac 660
ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
720 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
tcgcgcatat 780 gaaatcacgc catgtagtgt attgaccgat tccttgcggt
ccgaatgggc cgaacccgct 840 cgtctggcta agatcggccg cagcgatcgc
atccatagcc tccgcgaccg gctgcagaac 900 agcgggcagt tcggtttcag
gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat 960 gcaataggtc
aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc 1020
ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
1080 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
attcttcgcc 1140 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
tcgatcagaa acttctcgac 1200 agacgtcgcg gtgagttcag gcttttccat
gggtatatct ccttcttaaa gttaaacaaa 1260 attatttcta gagggaaacc
gttgtggtct ccctatagtg agtcgtatta atttcgcggg 1320 atcgagatcg
atccaattcc aatcccacaa aaatctgagc ttaacagcac agttgctcct 1380
ctcagagcag aatcgggtat tcaacaccct catatcaact actacgttgt gtataacggt
1440 ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa
cggcgttccc 1500 ggagttgcac acaagaaatt tgccactatt acagaggcaa
gagcagcagc tgacgcgtac 1560 acaacaagtc agcaaacaga caggttgaac
ttcatcccca aaggagaagc tcaactcaag 1620 cccaagagct ttgctaaggc
cctaacaagc ccaccaaagc aaaaagccca ctggctcacg 1680 ctaggaacca
aaaggcccag cagtgatcca gccccaaaag agatctcctt tgccccggag 1740
attacaatgg acgatttcct ctatctttac gatctaggaa ggaagttcga aggtgaaggt
1800 gacgacacta tgttcaccac tgataatgag aaggttagcc tcttcaattt
cagaaagaat 1860 gctgacccac agatggttag agaggcctac gcagcaggtc
tcatcaagac gatctacccg 1920 agtaacaatc tccaggagat caaatacctt
cccaagaagg ttaaagatgc agtcaaaaga 1980 ttcaggacta attgcatcaa
gaacacagag aaagacatat ttctcaagat cagaagtact 2040 attccagtat
ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga 2100
gtctctaaaa aggtagttcc tactgaatct aaggccatgc atggagtcta agattcaaat
2160 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga
gtcttttacg 2220 actcaatgac aagaagaaaa tcttcgtcaa catggtggag
cacgacactc tggtctactc 2280 caaaaatgtc aaagatacag tctcagaaga
ccaaagggct attgagactt ttcaacaaag 2340 gataatttcg ggaaacctcc
tcggattcca ttgcccagct atctgtcact tcatcgaaag 2400 gacagtagaa
aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat 2460
cattcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
2520 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
gtgacatctc 2580 cactgacgta agggatgacg cacaatccca ctatccttcg
caagaccctt cctctatata 2640 aggaagttca tttcatttgg agaggacacg
ctcgagctca tttctctatt acttcagcca 2700 taacaaaaga actcttttct
cttcttatta aaccatgaaa aagcctgaac tcaccgcgac 2760 gtctgtcgag
aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 2820
ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
2880 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
actttgcatc 2940 ggccgcgctc ccgattccgg aagtgcttga cattggggaa
ttcagcgaga gcctgaccta 3000 ttgcatctcc cgccgtgcac agggtgtcac
gttgcaagac ctgcctgaaa ccgaactgcc 3060 cgctgttctg cagccggtcg
cggaggccat ggatgcgatc gctgcggccg atcttagcca 3120 gacgagcggg
ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 3180
tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
3240 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
aggactgccc 3300 cgaagtccgg cacctcgtgc acgcggattt cggctccaac
aatgtcctga cggacaatgg 3360 ccgcataaca gcggtcattg actggagcga
ggcgatgttc ggggattccc aatacgaggt 3420 cgccaacatc ttcttctgga
ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 3480 cgagcggagg
catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 3540
tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
3600 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
gtacacaaat 3660 cgcccgcaga agcgcggccg tctggaccga tggctgtgta
gaagtactcg ccgatagtgg 3720 aaaccgacgc cccagcactc gtccgagggc
aaaggaatag tgaggtacct aaagaaggag 3780 tgcgtcgaag cagatcgttc
aaacatttgg caataaagtt tcttaagatt gaatcctgtt 3840 gccggtcttg
cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt 3900
aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
3960 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
attatcgcgc 4020 gcggtgtcat ctatgttact agatcgatgt cgaatcgatc
aacctgcatt aatgaatcgg 4080 ccaacgcgcg gggagaggcg gtttgcgtat
tgggcgctct tccgcttcct cgctcactga 4140 ctcgctgcgc tcggtcgttc
ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4200 acggttatcc
acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4260
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
4320 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
caggactata 4380 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
tctcctgttc cgaccctgcc 4440 gcttaccgga tacctgtccg cctttctccc
ttcgggaagc gtggcgcttt ctcaatgctc 4500 acgctgtagg tatctcagtt
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4560
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
4620 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
gcagagcgag 4680 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
aactacggct acactagaag 4740 gacagtattt ggtatctgcg ctctgctgaa
gccagttacc ttcggaaaaa gagttggtag 4800 ctcttgatcc ggcaaacaaa
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4860 gattacgcgc
agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 4920
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgacattaa cctataaaaa
4980 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
aaaacctctg 5040 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
gcggatgccg ggagcagaca 5100 agcccgtcag ggcgcgtcag cgggtgttgg
cgggtgtcgg ggctggctta actatgcggc 5160 atcagagcag attgtactga
gagtgcacca tatggacata ttgtcgttag aacgcggcta 5220 caattaatac
ataaccttat gtatcataca catacgattt aggtgacact atagaacggc 5280
gcgccaagct tggatctcct gcagcccggg ggatccgccc acgtacggta ccatctgcta
5340 atattttaaa tcacatgcaa gagaggaggc atggttccat tttctacctt
cacattattt 5400 gagaaaaacg aacttgttct gtgttttatt tttgcccttc
acattagtac aacgtggaag 5460 actcatggtt acacagaatc atacataagt
acaatgcttg tccctaagaa aacaagcact 5520 cgttgtattg aacctttacg
gctcatgcgg ccgcgaattc actagtgatt gaattcgcgg 5580 ccgcttagtc
cgacttggcc ttggcggccg cggccgactc tttgagcgtg aagatctgcg 5640
ccgtctcggg cacagcgccg tagttgacaa agaggtgcgc ggtcttgaag aaggccgtga
5700 tgatgggctc gtcgttcctg cgcacgaggt gcgggtacgc ggccgcaaag
tgcttggtgg 5760 cttcgttgag cttgtagtgc ggaatgatcg ggaacaagtg
gtggacctgg tgcgtgccaa 5820 tgtggtggct caggttgtcc acgaacgcgc
cgtacgagcg gtcgacgctc gagaggttgc 5880 ccttgacgta cgtccactcc
gagtcgccgt accacggcgt cgcttcgtcg ttgtggtgca 5940 agaaggtcgt
aatgacgagg aacgaagcaa agacaaagag cggcgcatag tagtagaggc 6000
ccatgacggc aaagccgagc gagtatgtga ggtacgcgta cgcggcgaag aaggcggccc
6060 agacgccgag cgacacgatg acggccgacg cgcggcgaag gaggagcggg
tcccacgggt 6120 caaagtggct catcgtgcgc ggggcatacc cgaccttcaa
gtagacaaac cacgcaccgc 6180 cgagcgtgta gacccattgg cgcacgtcct
ggaggtcctt gaccgaccgg tgcgggtaaa 6240 agatctcgtc cttatcaatg
ttgcccgtgt tcttgtggtg gtggcggtgc gtcacgcgcc 6300 agctctcgaa
cggcgtcaaa atcgcagagt gcatgatgca gccgatgata aagttgacgc 6360
tgtggtagcg cgagaaggcc gagtggccgc agtcgtggcc gaccgtgaag aagccccaga
6420 agatgacgcc ctgcacgtag atgtaggtgg cgcaaacgag cgcgtggagc
agaacgttat 6480 cggcaatgaa cggcgtcgag cgcgccgcgt agagcagcgc
cgccgaggcc gacgcgttga 6540 agatcgcgcg ggccgtgtag tagagcgaga
ggccgaggtt cgactcaaag cacgcgttcg 6600 ggatcgagtg cttgagctcc
gtgagcgtcg ggaactcgac cttcgtctta tcctcagtca 6660 tgcggccgct
gaagtattgc ttcttagtta acctttcctt tctctctcag ctatgtgaat 6720
tcattttgct ttcgtcacaa tttatatagt gaaattggat ctttggagtt aacgccttca
6780 caggattatc gtgttagaac aatgcttttt catgttctaa ttagtagtac
attacaaatg 6840 tgcactctat tcaataagca tcttttggca cgttaataaa
tcatgtgaaa aaaaaatact 6900 actatttcaa agaaagtgtt gtaaaaagaa
acggaaagag agctggcttc agttgttgag 6960 acttgtttgc tagtaaaaat
ggtgtgaaga gtgattcatg gtgaggtggt ttttcgtccc 7020 tttctgtttg
catgaaaaac aaatggcaag agatgacgta ggattccttc ccttaacgat 7080
tatctgtttt taatttcaaa tatacatata ggaatttatg aattactaag gttgtaaaat
7140 atgctggtca tttatttatg gctaaaatat ttttttttct cgtaaatata
aaaatattta 7200 aaatttattt ttatcatatt ttttatcctt ataaaattat
gtgtacaacc tatataaaaa 7260 aatatcatat ttaatattga ttatatgttt
aatcaatata aaaaatcatt atcatatatt 7320 tagatttatt cgaatataca
tctaaacaaa aaataacata ttttaatttt atgaagaaaa 7380 aaaaatattt
tatcctttat ttatttaaga ttaattaata gttatgtatt gtggaaagac 7440
ttttacacat gcaatagata tactgaatca attagatgcc aatgctgagt tggaaatcac
7500 ttgaggaggg gaggagactt gccaatgctt ttcagtttca tttaaatgat
ttagtggagg 7560 agatagagta gtgataaagg catgccccaa ttttggagtg
tatatatgag tggaaataag 7620 agagggatag agagaaaaaa taaagagagt
aaaaataatt aatgtgaaat gatatgataa 7680 aaaaataaag aaagagataa
agagaaaaat gaaatgagag atagatgaaa tagagagtag 7740 atacatgttt
gtttaggttt tttttaggaa ataacacatt tttttctcat cacttattac 7800
tcactgtcaa tttcctctct ttcaatcata atgatatgat ttgtttaaca aaaatgtgaa
7860 aaaacatata aagtaaaata tttttataaa ttgataaata aaaatttaca
aaatttattt 7920 cttattaaat tgaatagaaa atgaaagaaa agaaaagaaa
aagtatatat aaaatgatat 7980 agctttaaaa agaataaatt tttcatatca
gtcttttttt aataatttag aaatatttaa 8040 gtatatagca aaaatataat
gtactttaca tatgcataaa taataatttg aaaatagaac 8100 taatagaata
gagaaaaaag taatataata attaactata tgaaaattta gaagggacaa 8160
tatttttaat taagaatata aacaatattt cttttcatgt aatgagggac ggatgtacgg
8220 ggccagtgtt ggagtcaaag ccaaaatagt cacggggaaa ttaatgcact
gcatgactat 8280 tcgaaaaaat tcactagcct tacttagatg ttagattaat
agctaggggg tgcagataat 8340 tttgaaaggc atgaaaaaca ttaatttgta
cattgcaagc ttttgatgac aagctttgca 8400 attgttcaca ctaccttatg
ccatttataa atagagtgat tggcatatga aggaaatcat 8460 gagagtcgaa
gcgaaaaaca aagcttgaga gtgtaggaaa aatacagttt ttttggtaaa 8520
aatacagtat ttgaatagga gcgaaaaata tcctttcaaa atgatccttt tctttttttt
8580 tttttttctt gttgttcttg gtcagttatt caaaggaaaa gggattgaaa
taaaaacttg 8640 catgtgggat cgtacgtcga gtcgacctgc a 8671 <210>
SEQ ID NO 94 <211> LENGTH: 1413 <212> TYPE: DNA
<213> ORGANISM: Saprolegnia diclina <400> SEQUENCE: 94
atggccccgc agacggagct ccgccagcgc cacgccgccg tcgccgagac gccggtggcc
60 ggcaagaagg cctttacatg gcaggaggtc gcgcagcaca acacggcggc
ctcggcctgg 120 atcattatcc gcggcaaggt ctacgacgtg accgagtggg
ccaacaagca ccccggcggc 180 cgcgagatgg tgctgctgca cgccggtcgc
gaggccaccg acacgttcga ctcgtaccac 240 ccgttcagcg acaaggccga
gtcgatcttg aacaagtatg agattggcac gttcacgggc 300 ccgtccgagt
ttccgacctt caagccggac acgggcttct acaaggagtg ccgcaagcgc 360
gttggcgagt acttcaagaa gaacaacctc catccgcagg acggcttccc gggcctctgg
420 cgcatgatgg tcgtgtttgc ggtcgccggc ctcgccttgt acggcatgca
cttttcgact 480 atctttgcgc tgcagctcgc ggccgcggcg ctctttggcg
tctgccaggc gctgccgctg 540 ctccacgtca tgcacgactc gtcgcacgcg
tcgtacacca acatgccgtt cttccattac 600 gtcgtcggcc gctttgccat
ggactggttt gccggcggct cgatggtgtc atggctcaac 660 cagcacgtcg
tgggccacca catctacacg aacgtcgcgg gctcggaccc ggatcttccg 720
gtcaacatgg acggcgacat ccgccgcatc gtgaaccgcc aggtgttcca gcccatgtac
780 gcattccagc acatctacct tccgccgctc tatggcgtgc ttggcctcaa
gttccgcatc 840 caggacttca ccgacacgtt cggctcgcac acgaacggcc
cgatccgcgt caacccgcac 900 gcgctctcga cgtggatggc catgatcagc
tccaagtcgt tctgggcctt ctaccgcgtg 960 taccttccgc ttgccgtgct
ccagatgccc atcaagacgt accttgcgat cttcttcctc 1020 gccgagtttg
tcacgggctg gtacctcgcg ttcaacttcc aagtaagcca tgtctcgacc 1080
gagtgcggct acccatgcgg cgacgaggcc aagatggcgc tccaggacga gtgggcagtc
1140 tcgcaggtca agacgtcggt cgactacgcc catggctcgt ggatgacgac
gttccttgcc 1200 ggcgcgctca actaccaggt cgtgcaccac ttgttcccca
gcgtgtcgca gtaccactac 1260 ccggcgatcg cgcccatcat cgtcgacgtc
tgcaaggagt acaacatcaa gtacgccatc 1320 ttgccggact ttacggcggc
gttcgttgcc cacttgaagc acctccgcaa catgggccag 1380 cagggcatcg
ccgccacgat ccacatgggc taa 1413
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