Anti-Inflammatory Peptides and Composition Comprising the Same

Abstract

The present invention relates to a peptide with anti-inflammatory activity, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology of amino acid sequence with above-mentioned sequences, or the peptide is the fragment of the above-mentioned peptides. The present invention also relates to an inflammatory composition comprising the above mentioned peptides. According to the present invention, a peptide that has at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161 has outstanding efficacy in both suppressing inflammation and in prophylactic means. Therefore, the composition comprising the peptides of this invention can be used as anti-inflammatory pharmaceutical compositions or as cosmetic compositions, in turn, treating and preventing a variety of different types of inflammatory diseases.

Description

FIELD OF THE INVENTION

[0001] The present invention relates to anti-inflammatory peptides and compositions comprising the same.

BACKGROUND OF THE INVENTION

[0002] Inflammation is a type of biological defense as a means of protecting the body from damage of biological tissues that could be caused by external physical stimuli, chemical stimuli such as exposure to various allergens, or invasion of microorganisms including bacteria, fungi and viruses.

[0003] The Cyclooxygenase(COX) pathway or Lipoxygenase(LOX) Pathway can used for signaling inflammation, which produce prostaglandin, thromboxane, etc. Once the inflammatory signal is delivered, one of many changes that happen in the body is the expansion of the blood vessel for increased blood supply around the inflammation to concentrate blood cells such as neutrophils required for the inflammatory response. However, inflammatory diseases can result if an abnormal biological defense response occurs excessively. To prevent this, drugs that suppress excessive inflammatory responses by repressing enzymes used in inflammatory signaling pathways (for example COX-1, COX-2, 5-LOX, 12-LOX etc.) are under development.

[0004] According to response time, inflammation is categorized as acute inflammation (immediate response, non-specific response, several days to several weeks), chronic inflammation (delayed response, specific response, several weeks or more), subacute inflammation (a middle stage in between acute inflammation and chronic inflammation, characteristics of mixed product of mononuclear and polymorphounuclear).

[0005] Also, aside from peptide factors, factors such as prostaglandin, leukotriene, lipid factors including platelet activating factor (PAF), synthetic enzyme of inflammation factor, free radical such as NO (nitric oxide), many kinds of cell adhesion molecules, the immune system, and coagulation factors can cause inflammation.

[0006] Once a cell is damaged due to the known causative agents of inflammation such as external biological factors (microbes, viruses, parasites), physical factors (mechanical stimuli, heat, radiation, electricity), and chemical factors, histamine and kinin are released. The released histamine and kinin will result in angiectasis, increased capillary permeability and concentration of macrophages at the inflammation site, and it causes increased blood flow rate, edema, immunocyte and antibody migration, pain and heat generation.

[0007] Currently used treatments for inflammation are synthetic drugs such as ibuprofen, antihistamines, steroids, cortisone, immunosuppressive agents, and immune agonist; those which only temporarily alleviate inflammation. These drugs do not fundamentally cure inflammation, and they have side effects such as hypersensitivity reaction, and deterioration of immune system,

[0008] Therefore, for effective alleviation of inflammation, research is being done to develop a substance that inhibits expression of the above mentioned inflammatory proteins. However, problems have arisen in anti-inflammation substances that had been developed previously. Diverse categories of anti-inflammatory drugs including Non-steroidal Anti-inflammatory Drugs (NSAIDs) and Steroidal Anti-inflammatory Drugs (SAIDs) have been developed; but not only do these drugs often bear side effects upon use, they also do not fundamentally cure the inflammation. Thus, there is a current need for anti-inflammatory drugs that are both physically and economically feasible. As one example, in acute or chronic inflammations such as chronic rheumatoid arthritis, not only do non-steroidal anti-inflammatory drugs suppress COX-2 enzyme activity, they are also known to suppress COX-1 activity, causing side effects such as gastrointestinal disorders.

[0009] The present invention was completed as the present inventors have found that peptides derived from telomerase can have anti-inflammatory properties.

[0010] Therefore the objective of this invention is to provide a novel peptide.

[0011] Another objective of present invention is to provide the polynucleotide that codes the novel peptide.

[0012] Another objective of present invention is to provide a peptide that has anti-inflammatory activity.

[0013] Another objective of present invention is to provide an anti-inflammatory composition that uses this peptide as an active ingredient.

[0014] Another objective of present invention is to provide a cosmetic composition that uses this peptide as an active ingredient.

[0015] Another objective of present invention is to provide a pharmaceutical composition that uses this peptide as an active ingredient.

SUMMARY OF THE INVENTION

[0016] In one embodiment the present invention relates to a peptide with anti-inflammatory activity, wherein the peptide comprises at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, or where the peptide has at least 80% sequence identity with the above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides.

[0017] In another embodiment, the above-mentioned fragment consists of 3 or more amino acids. For instance, the fragment may consist of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, or 26 amino acid residues.

[0018] In another embodiment, the above-mentioned peptide consists of 30 or less amino acids. For instance, the peptide may consist of 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, or 8 amino acid residues.

[0019] In another embodiment, the above-mentioned peptide consists of any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161.

[0020] In another embodiment, the above-mentioned peptide comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1 to SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 14 to 21, SEQ ID NO: 23 to SEQ ID NO: 37, SEQ ID NO: 39 to SEQ ID NO: 44, SEQ ID NO: 47 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 61, SEQ ID NO: 63 to SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 99 to SEQ ID NO: 104, SEQ ID NO: 107 to SEQ ID NO: 109, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 120 to SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 129 to SEQ ID NO: 133, SEQ ID NO: 142 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, and SEQ ID NO: 155 to SEQ ID NO: 159.

[0021] In another embodiment, the above-mentioned peptide comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 15 to SEQ ID NO: 18, SEQ ID NO: 23 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31 to SEQ ID NO: 34, SEQ ID NO: 39 to SEQ ID NO: 41, SEQ ID NO: 47, SEQ ID NO:48, SEQ ID NO: 51 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 58, SEQ ID NO: 61, SEQ ID NO: 65 to SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 73 to SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 87, SEQ ID NO: 90 to SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 99, SEQ ID NO: 101 to SEQ ID NO: 104, SEQ ID NO: 107 to SEQ ID NO: 109, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 129 to SEQ ID NO: 132, SEQ ID NO: 142 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, and SEQ ID NO: 157 to SEQ ID NO: 159.

[0022] In another embodiment, the above-mentioned peptide comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1 to SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 32 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 60, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72 to SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 99 to SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 127 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 151 and SEQ ID NO: 153 to SEQ ID NO: 161.

[0023] In another embodiment, the above-mentioned peptide comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1 to SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17 to SEQ ID NO: 23, SEQ ID NO: 25 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 33 to SEQ ID NO: 43, SEQ ID NO: 156, SEQ ID NO: 157 and SEQ ID NO: 159.

[0024] In another embodiment, the above-mentioned peptide comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 52, SEQ ID NO: 57, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 91, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 118, SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 129, SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 135, SEQ ID NO: 146, SEQ ID NO: 151, SEQ ID NO: 154, and SEQ ID NO: 156.

[0025] In another embodiment, the above-mentioned peptide originates from human telomerase.

[0026] In one embodiment of the present invention, a polynucleotide encoding a peptide with anti-inflammatory activity, wherein the peptide comprises at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, or the peptide has at least 80% sequence identity with the above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides, is provided.

[0027] In another embodiment of the polynucleotide, the above-mentioned peptide consists of 30 or less amino acids. For instance, the peptide may consist of 29, 28, 27, 26, 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, or 8 amino acid residues.

[0028] In another embodiment of the polynucleotide, the above-mentioned peptide consists of any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161.

[0029] In another embodiment of the polynucleotide, the above-mentioned peptide originates from human telomerase.

[0030] In one embodiment of the present invention, anti-inflammatory composition comprising a peptide as active ingredient, wherein the peptide comprises at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology of amino acid sequence with above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides, is provided.

[0031] In another embodiment of the composition, the above-mentioned peptide consists of 30 or less amino acids, cf. above.

[0032] In another embodiment of the composition, the above-mentioned peptide consists of any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161.

[0033] In another embodiment of the composition, the above-mentioned peptide originates from human telomerase.

[0034] In another embodiment of the composition, the above-mentioned composition is for treatment or prophylaxis of inflammatory disease.

[0035] In another embodiment of the composition, the above-mentioned composition is a cosmetic composition for improving or preventing skin inflammation.

[0036] In another embodiment of the composition, the above-mentioned composition is a pharmaceutical composition for treatment or prophylaxis of inflammatory diseases.

[0037] In another embodiment of the composition, the above-mentioned composition is a food composition for treatment or prophylaxis of inflammation.

[0038] In another embodiment of the composition, the above-mentioned inflammatory disease is characterized by selecting from the group consisting of (1) general or localized inflammatory disease (for example, allergies; immune-complex disease; hayfever; hypersensitive shock; endotoxin shock; cachexia, hyperthermia; granulomatosis; or sarcoidosis); (2) gastro-intestinal related diseases (for example, appendicitis; gastric ulcer; duodenal ulcer; peritonitis; pancreatitis; ulcerative, acute, or ischemic colitis; cholangitis; cholecystitis, steatorrhea, hepatitis, Crone's disease; or Whipple's Disease); (3) dermal related diseases (for example, psoriasis; burns; sunburns; dermatitis; Urticarial warts or wheal); (4) vascular related diseases (for example, angiitis; vasculitis; endocarditis; arteritis; atherosclerosis; thrombophlebitis; pericarditis; congestive heart failure; myocarditis; myocardial ischemia; periarteritis nodosa; recurrent stenosis; Buerger's disease; or rheumatic fever); (5) respiratory diseases (for example, asthma; epiglottitis; bronchitis; emphysema; rhinitis; cystic fibrosis; interstitial pneumonitis; COPD (chronic obstructive pulmonary disease); adult respiratory distress syndrome; coniosis; alveolitis; bronchiolitis; pharyngitis; pleurisy; or sinusitis); (6) bone, joint, muscle and connective tissue related diseases (for example, eosinophilic granuloma; arthritis; arthralgia; osteomyelitis; dermatomyositis; fasciitis; Paget's disease; gout; periodontal disease; rheumatoid arthritis; myasthenia gravis; ankylosing spondylitis; or synovitis); (7) urogenital disorders (for example, epididymitis; vaginitis; prostatitis; or urethritis); (8) central or peripheral nervous system related diseases (for example, Alzheimer's disease; meningitis; encephalitis; multiple sclerosis; cerebral infarction; cerebral embolism; Guillain-Barre syndrome; neuritis; neuralgia; spinal cord injury; paralysis; or uveitis); (9) virus (for example, influenza; respiratory syncytial virus; HIV; hepatitis B; hepatitis C; or herpes virus), infectious disease (for example, Dengue fever; or septicemia), fungal infection (for example, candidiasis); or bacterial, parasitic, and similar microbial infection (for example, disseminated bacteremia; malaria; onchocerciasis; or amebiasis); (10) autoimmune disease (for example, thyroiditis; lupus; Goodpasture's syndrome; allograft rejection; graft versus host disease; or diabetes); and (11) cancer or tumor disease (for example, Hodgkin's disease).

[0039] In one embodiment of the present invention, a method for treating or preventing inflammatory diseases by administering the anti-inflammatory composition is provided.

[0040] In one embodiment of the present invention, a kit for prophylaxis or treatment of inflammatory diseases comprising: a peptide with anti-inflammatory activity or a composition comprising of the peptide, wherein the peptide comprises at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology with above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides; and instructions including at least one of administration dose, administration route, administration frequency, and indication of the peptide or composition, is provided.

INDUSTRIAL APPLICABILITY

[0041] According to the present invention, a peptide that has a sequence of SEQ ID NO: 1 to SEQ ID NO: 161 has outstanding efficacy in both suppressing inflammation and in prophylactic means. Therefore, the composition comprising the peptides of this invention can be used as anti-inflammatory pharmaceutical composition or as cosmetic composition, in turn, treating and preventing a variety of different types of inflammatory diseases.

REFERENCES

[0042] KR2012-0130996A [0043] KR2012-0133661A [0044] KR2011-0060940A [0045] US2011-0150873A1 [0046] Bonaldi T et al., EMBO J, (22)5551-60, 2003 [0047] Yankner B A et al, Science (New York, N.Y.) [1990, 250(4978):279-282] [0048] Dahlgren K N et al, J. Biol. Chem. 277:32046-32053, 2002.

BRIEF DESCRIPTION OF THE DRAWINGS

[0049] FIG. 1 to FIG. 16 are results from screening TNF-.alpha. inhibition effects on monocytes.

[0050] FIG. 17 to FIG. 35 are results from screening TNF-.alpha. inhibition effects on cell line THP-1.

[0051] FIG. 36 to FIG. 108 are the western blot results of selected peptides which showed accumulation of HMGB1 in the cell.

DETAILED DESCRIPTION OF THE INVENTION

[0052] Since the present invention can have adaptability for diverse transformation and examples of practical application, below is a more detailed description of the present invention. Nevertheless, this is no means to limit the form of practical application; it should be understood that the intention is to include the concept and the extent of technology in all of the transformation, equivalents to alternatives. In describing the present invention, if any detailed description about the prior art is considered to deteriorate the fundamental principles of the present invention, the description will be omitted.

[0053] A telomere is known as a repetitive sequence of genetic material at the ends of chromosomes that prevent chromosomes from damage or merging of other chromosomes. The length of a telomere is shortened at each cell division, and after a certain number of cell division, the telomere length is extremely shortened to the extent in which the cell stops dividing and dies. On the other hand, the elongation of telomeres is known to extend the life span of a cell. For an example, cancer cells excrete an enzyme called telomerase, which prevents shortening of telomeres, thus resulting in proliferation of cancer cells. The present invention was accomplished upon the discovery of telomerase-derived peptides with anti-inflammatory effects.

[0054] In one embodiment of the present invention, a peptide with anti-inflammatory activities is provided. The peptide comprises at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology with above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides.

[0055] The peptides described in SEQ ID NO: 1 to SEQ ID NO: 161 are as the following table 1. SEQ ID NO: 162 lists the order of full length of human telomerase protein. SEQ ID NO: 163 lists the telomerase-derived peptide that consists of 16 amino acid sequence.

[0056] The "name" in Table 1 below was used for distinction of peptides. In a different specific embodiment of the present invention, more than one peptide of the mentioned peptides in SEQ ID NO: 1 to SEQ ID NO: 161 comprise a "synthetic peptide", a synthesized peptide of selected areas of the telomerase. In the present specification, the term "pep" herein relates to a peptide that has any one of the SEQ ID NO: 1 to SEQ ID NO: 161, or, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or a peptide fragment of above-mentioned peptides.

TABLE-US-00001 TABLE 1 SEQ POSITION ID IN NO NAME TELOMERASE SEQUENCE LENGTH 1. pep2 [660-689] ALFSVLNYERARRPGLLGASVLGLDDIHRA 30 aa 2. pep3 [663-677] SVLNYERARRPGLLG 15 aa 3. pep4 [674-683] GLLGASVLGL 10 aa 4. pep5 [615-624] ALLTSRLRFI 10 aa 5. pep6 [613-621] RPALLTSRL 9 aa 6. pep7 [653-661] RLTSRVKAL 9 aa 7. pep8 [691-705] RTFVLRVRAQDPPPE 15 aa 8. pep9 [653-667] RLTSRVKALFSVLNY 15 aa 9. pep10 [651-665] AERLTSRVKALFSVL 15 aa 10. pep11 [667-675] YERARRPGL 9 aa 11. pep12 [675-683] LLGASVLGL 9 aa 12. pep13 [680-689] VLGLDDIHRA 10 aa 13. pep14 [677-686] GASVLGLDDI 10 aa 14. pep15 [660-669] ALFSVLNYER 10 aa 15. pep16 [663-672] SVLNYERARR 10 aa 16. pep17 [679-688] SVLGLDDIHR 10 aa 17. pep18 [662-671] FSVLNYERAR 10 aa 18. pep19 [666-675] NYERARRPGL 10 aa 19. pep20 [667-676] YERARRPGLL 10 aa 20. pep21 [672-681] RPGLLGASVL 10 aa 21. pep22 [668-676] ERARRPGLL 9 aa 22. pep23 [680-688] VLGLDDIHR 9 aa 23. pep24 [663-671] SVLNYERAR 9 aa 24. pep25 [664-672] VLNYERARR 9 aa 25. pep26 [670-678] ARRPGLLGA 9 aa 26. pep27 [673-681] PGLLGASVL 9 aa 27. pep28 [671-679] RRPGLLGAS 9 aa 28. pep29 [660-668] ALFSVLNYE 9 aa 29. pep30 [674-682] GLLGASVLG 9 aa 30. pep31 [679-687] SVLGLDDIH 9 aa 31. pep32 [668-675] ERARRPGL 8 aa 32. pep33 [670-677] ARRPGLLG 8 aa 33. pep34 [674-681] GLLGASVL 8 aa 34. pep35 [669-676] RARRPGLL 8 aa 35. pep36 [676-683] LGASVLGL 8 aa 36. pep37 [563-577] VTETTFQKNRLFFYR 15 aa 37. pep38 [573-587] LFFYRKSVWSKLQSI 15 aa 38. pep39 [583-597] KLQSIGIRQHLKRVQ 15 aa 39. pep40 [603-617] EAEVRQHREARPALL 15 aa 40. pep41 [613-627] RPALLTSRLRFIPKP 15 aa 41. pep42 [623-637] FIPKPDGLRPIVNMD 15 aa 42. pep43 [643-657] RTFRREKRAERLTSR 15 aa 43. pep45 [683-697] LDDIHRAWRTFVLRV 15 aa 44. pep46 [693-707] FVLRVRAQDPPPELY 15 aa 45. pep47 [721-735] PQDRLTEVIASIIKP 15 aa 46. pep48 [578-592] KSVWSKLQSIGIRQH 15 aa 47. pep49 [593-608] LKRVQLRELSEAEVRQ 16 aa 48. pep50 [1-20] MPRAPRCRAVRSLLRSHYRE 20 aa 49. pep51 [21-40] VLPLATFVRRLGPQGWRLVQ 20 aa 50. pep52 [41-60] RGDPAAFRALVAQCLVCVPW 20 aa 51. pep53 [61-80] DARPPPAAPSFRQVSCLKEL 20 aa 52. pep54 [81-100] VARVLQRLCERGAKNVLAFG 20 aa 53. pep55 [101-120] FALLDGARGGPPEAFTTSVR 20 aa 54. pep56 [121-140] SYLPNTVTDALRGSGAWGLL 20 aa 55. pep57 [141-160] LRRVGDDVLVHLLARCALFV 20 aa 56. pep58 [161-180] LVAPSCAYQVCGPPLYQLGA 20 aa 57. pep59 [181-200] ATQARPPPHASGPRRRLGCE 20 aa 58. pep60 [201-220] RAWNHSVREAGVPLGLPAPG 20 aa 59. pep61 [221-240] ARRRGGSASRSLPLPKRPRR 20 aa 60. pep62 [241-260] GAAPEPERTPVGQGSWAHPG 20 aa 61. pep63 [261-280] RTRGPSDRGFCVVSPARPAE 20 aa 62. pep64 [281-300] EATSLEGALSGTRHSHPSVG 20 aa 63. pep65 [301-320] RQHHAGPPSTSRPPRPWDTP 20 aa 64. pep66 [321-340] CPPVYAETKHFLYSSGDKEQ 20 aa 65. pep67 [341-360] LRPSFLLSSLRPSLTGARRL 20 aa 66. pep68 [361-380] VETIFLGSRPWMPGTPRRLP 20 aa 67. pep69 [381-400] RLPQRYWQMRPLFLELLGNH 20 aa 68. pep70 [401-420] AQCPYGVLLKTHCPLRAAVT 20 aa 69. pep71 [421-440] PAAGVCAREKPQGSVAAPEE 20 aa 70. pep72 [441-460] EDTDPRRLVQLLRQHSSPWQ 20 aa 71. pep73 [461-480] VYGFVRACLRRLVPPGLWGS 20 aa 72. pep74 [481-500] RHNERRFLRNTKKFISLGKH 20 aa 73. pep75 [501-520] AKLSLQELTWKMSVRDCAWL 20 aa 74. pep76 [521-540] RRSPGVGCVPAAEHRLREEI 20 aa 75. pep77 [541-560] LAKFLHWLMSVYVVELLRSF 20 aa 76. pep78 [561-580] FYVTETTFQKNRLFFYRKSV 20 aa 77. pep79 [581-600] WSKLQSIGIRQHLKRVQLRE 20 aa 78. pep80 [601-620] LSEAEVRQHREARPALLTSR 20 aa 79. pep81 [621-640] LRFIPKPDGLRPIVNMDYVV 20 aa 80. pep82 [641-660] GARTFRREKRAERLTSRVKA 20 aa 81. pep83 [661-680] LFSVLNYERARRPGLLGASV 20 aa 82. pep84 [681-700] LGLDDIHRAWRTFVLRVRAQ 20 aa 83. pep85 [701-720] DPPPELYFVKVDVTGAYDTI 20 aa 84. pep86 [721-740] PQDRLTEVIASIIKPQNTYC 20 aa 85. pep87 [741-760] VRRYAVVQKAAHGHVRKAFK 20 aa 86. pep88 [761-780] SHVSTLTDLQPYMRQFVAHL 20 aa 87. pep89 [781-800] QETSPLRDAVVIEQSSSLNE 20 aa 88. pep90 [801-820] ASSGLFDVFLRFMCHHAVRI 20 aa 89. pep91 [821-840] RGKSYVQCQGIPQGSILSTL 20 aa 90. pep92 [841-860] LCSLCYGDMENKLFAGIRRD 20 aa 91. pep93 [861-880] GLLLRLVDDFLLVTPHLTHA 20 aa 92. pep94 [881-900] KTFLRTLVRGVPEYGCVVNL 20 aa 93. pep95 [901-920] RKTVVNFPVEDEALGGTAFV 20 aa 94. pep96 [921-940] QMPAHGLFPWCGLLLDTRTL 20 aa 95. pep97 [941-960] EVQSDYSSYARTSIRASLTF 20 aa 96. pep98 [961-980] NRGFKAGRNMRRKLFGVLRL 20 aa 97. pep99 [981-1000] KCHSLFLDLQVNSLQTVCTN 20 aa 98. pep100 [1001-1020] IYKILLLQAYRFHACVLQLP 20 aa 99. pep101 [1021-1040] FHQQVWKNPTFFLRVISDTA 20 aa 100. pep102 [1041-1060] SLCYSILKAKNAGMSLGAKG 20 aa 101. pep103 [1061-1080] AAGPLPSEAVQWLCHQAFLL 20 aa 102. pep104 [1081-1100] KLTRHRVTYVPLLGSLRTAQ 20 aa 103. pep105 [1101-1120] TQLSRKLPGTTLTALEAAAN 20 aa 104. pep106 [1121-1132] PALPSDFKTILD 12 aa 105. pep107 [1-10] MPRAPRCRAV 10 aa 106. pep108 [11-30] RSLLRSHYREVLPLATFVRR 20 aa 107. pep109 [31-50] LGPQGWRLVQRGDPAAFRAL 20 aa 108. pep110 [51-70] VAQCLVCVPWDARPPPAAPS 20 aa 109. pep111 [71-90] FRQVSCLKELVARVLQRLCE 20 aa 110. pep112 [91-110] RGAKNVLAFGFALLDGARGG 20 aa 111. pep113 [111-130] PPEAFTTSVRSYLPNTVTDA 20 aa 112. pep114 [131-150] LRGSGAWGLLLRRVGDDVLV 20 aa 113. pep115 [151-170] HLLARCALFVLVAPSCAYQV 20 aa 114. pep116 [171-190] CGPPLYQLGAATQARPPPHA 20 aa 115. pep117 [191-210] SGPRRRLGCERAWNHSVREA 20 aa 116. pep118 [211-230] GVPLGLPAPGARRRGGSASR 20 aa 117. pep119 [231-250] SLPLPKRPRRGAAPEPERTP 20 aa 118. pep120 [251-270] VGQGSWAHPGRTRGPSDRGF 20 aa 119. pep121 [271-290] CVVSPARPAEEATSLEGALS 20 aa 120. pep122 [291-310] GTRHSHPSVGRQHHAGPPST 20 aa 121. pep123 [311-330] SRPPRPWDTPCPPVYAETKH 20 aa 122. pep124 [331-350] FLYSSGDKEQLRPSFLLSSL 20 aa

123. pep125 [351-370] RPSLTGARRLVETIFLGSRP 20 aa 124. pep126 [371-390] WMPGTPRRLPRLPQRYWQMR 20 aa 125. pep127 [391-410] PLFLELLGNHAQCPYGVLLK 20 aa 126. pep128 [411-430] THCPLRAAVTPAAGVCAREK 20 aa 127. pep129 [431-450] PQGSVAAPEEEDTDPRRLVQ 20 aa 128. pep130 [451-470] LLRQHSSPWQVYGFVRACLR 20 aa 129. pep131 [471-490] RLVPPGLWGSRHNERRFLRN 20 aa 130. pep132 [491-510] TKKFISLGKHAKLSLQELTW 20 aa 131. pep133 [511-530] KMSVRDCAWLRRSPGVGCVP 20 aa 132. pep134 [531-550] AAEHRLREEILAKFLHWLMS 20 aa 133. pep135 [551-570] VYVVELLRSFFYVTETTFQK 20 aa 134. pep136 [571-590] NRLFFYRKSVWSKLQSIGIR 20 aa 135. pep137 [591-610] QHLKRVQLRELSEAEVRQHR 20 aa 136. pep138 [611-630] EARPALLTSRLRFIPKPDGL 20 aa 137. pep139 [631-650] RPIVNMDYVVGARTFRREKR 20 aa 138. pep140 [651-670] AERLTSRVKALFSVLNYERA 20 aa 139. pep141 [671-690] RRPGLLGASVLGLDDIHRAW 20 aa 140. pep142 [691-710] RTFVLRVRAQDPPPELYFVK 20 aa 141. pep143 [711-730] VDVTGAYDTIPQDRLTEVIA 20 aa 142. pep144 [731-750] SIIKPQNTYCVRRYAVVQKA 20 aa 143. pep145 [751-770] AHGHVRKAFKSHVSTLTDLQ 20 aa 144. pep146 [771-790] PYMRQFVAHLQETSPLRDAV 20 aa 145. pep147 [791-810] VIEQSSSLNEASSGLFDVFL 20 aa 146. pep148 [811-830] RFMCHHAVRIRGKSYVQCQG 20 aa 147. pep149 [831-850] IPQGSILSTLLCSLCYGDME 20 aa 148. pep150 [851-870] NKLFAGIRRDGLLLRLVDDF 20 aa 149. pep151 [871-890] LLVTPHLTHAKTFLRTLVRG 20 aa 150. pep152 [891-910] VPEYGCVVNLRKTVVNFPVE 20 aa 151. pep153 [911-930] DEALGGTAFVQMPAHGLFPW 20 aa 152. pep154 [931-950] CGLLLDTRTLEVQSDYSSYA 20 aa 153. pep155 [951-970] RTSIRASLTFNRGFKAGRNM 20 aa 154. pep156 [971-990] RRKLFGVLRLKCHSLFLDLQ 20 aa 155. pep157 [991-1010] VNSLQTVCTNIYKILLLQAY 20 aa 156. pep158 [1011-1030] RFHACVLQLPFHQQVWKNPT 20 aa 157. pep159 [1031-1050] FFLRVISDTASLCYSILKAK 20 aa 158. pep160 [1051-1070] NAGMSLGAKGAAGPLPSEAV 20 aa 159. pep161 [1071-1090] QWLCHQAFLLKLTRHRVTYV 20 aa 160. pep162 [1091-1110] PLLGSLRTAQTQLSRKLPGT 20 aa 161. pep163 [1111-1132] TLTALEAAANPALPSDFKTILD 22 aa 162. Telomerase [1-1132] MPRAPRCRAVRSLLRSHYREVLPLATFVRR 1132 aa LGPQGWRLVQRGDPAAFRALVAQCLVCVPW DARPPPAAPSFRQVSCLKELVARVLQRLCERG AKNVLAFGFALLDGARGGPPEAFTTSVRSYLP NTVTDALRGSGAWGLLLRRVGDDVLVHLLAR CALFVLVAPSCAYQVCGPPLYQLGAATQARPP PHASGPRRRLGCERAWNHSVREAGVPLGLPA PGARRRGGSASRSLPLPKRPRRGAAPEPERTP VGQGSWAHPGRTRGPSDRGFCVVSPARPAE EATSLEGALSGTRHSHPSVGRQHHAGPPSTS RPPRPWDTPCPPVYAETKHFLYSSGDKEQLR PSFLLSSLRPSLTGARRLVETIFLGSRPWMPG TPRRLPRLPQRYWQMRPLFLELLGNHAQCPY GVLLKTHCPLRAAVTPAAGVCAREKPQGSVA APEEEDTDPRRLVQLLRQHSSPWQVYGFVRA CLRRLVPPGLWGSRHNERRFLRNTKKFISLG KHAKLSLQELTWKMSVRDCAWLRRSPGVGC VPAAEHRLREEILAKFLHWLMSVYVVELLRSF FYVTETTFQKNRLFFYRKSVWSKLQSIGIRQH LKRVQLRELSEAEVRQHREARPALLTSRLRFI PKPDGLRPIVNMDYVVGARTFRREKRAERLT SRVKALFSVLNYERARRPGLLGASVLGLDDIH RAWRTFVLRVRAQDPPPELYFVKVDVTGAYD TIPQDRLTEVIASIIKPQNTYCVRRYAVVQKA AHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQ ETSPLRDAVVIEQSSSLNEASSGLFDVFLRFM CHHAVRIRGKSYVQCQGIPQGSILSTLLCSLC YGDMENKLFAGIRRDGLLLRLVDDFLLVTPHL THAKTFLRTLVRGVPEYGCVVNLRKTVVNFPV EDEALGGTAFVQMPAHGLFPWCGLLLDTRTL EVQSDYSSYARTSIRASLTFNRGFKAGRNMR RKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYK ILLLQAYRFHACVLQLPFHQQVWKNPTFFLRV ISDTASLCYSILKAKNAGMSLGAKGAAGPLPS EAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTA QTQLSRKLPGTTLTALEAAANPALPSDFKTIL D 163. pep 1 [611-626] EARPALLTSRLRFIPK 16 aa

[0057] In one embodiment of the present invention, a polynucleotide that codes a peptide with anti-inflammatory activities is provided. The polynucleotide codes a peptide comprising at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, a peptide having above 80% homology with above-mentioned sequences, or a peptide being a fragment of the above-mentioned peptides. The polynucleotide mentioned above enables production of the peptides in large quantities. For example, cultivation of vectors that include polynucleotides encoding peptides allows production of peptides in large quantities.

[0058] The peptides disclosed herein can include a peptide comprising amino acid sequence above 80%, above 85%, above 90%, above 95%, above 96%, above 97%, above 98%, or above 99% homology. Moreover, the peptides disclosed in the present invention can include a peptide comprising SEQ ID NO: 1 or its fragments, and a peptide with more than 1 transformed amino acid, more than 2 transformed amino acid, more than 3 transformed amino acid, more than 4 transformed amino acid, more than 5 transformed amino acid, more than 6 transformed amino acid, or more than 7 transformed amino acid.

[0059] In the present specification and claims, the terms "homology" and "sequence identity" are used interchangeably to indicate the degree of sequence overlap between two amino acid (or if relevant: nucleic acid) sequences.

[0060] Unless otherwise stated the term "Sequence identity" for peptides as used herein refers to the sequence identity calculated as (n.sub.ref-n.sub.dif)100/n.sub.ref, wherein n.sub.dif is the total number of non-identical residues in the two sequences when aligned so that a maximum number of amino acids are identical and wherein n.sub.ref is the number of residues in the shortest of the sequences. Hence, the DNA sequence agtcagtc will have a sequence identity of 75% with the sequence aatcaatc (n.sub.dif=2 and n.sub.ref=8).

[0061] In some embodiments the sequence identity is determined by conventional methods, e.g., Smith and Waterman, 1981, Adv. Appl. Math. 2:482, by the search for similarity method of Pearson & Lipman, 1988, Proc. Natl. Acad. Sci. USA 85:2444, using the CLUSTAL W algorithm of Thompson et al., 1994, Nucleic Acids Res 22:467380, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group). The BLAST algorithm (Altschul et al., 1990, Mol. Biol. 215:403-10) for which software may be obtained through the National Center for Biotechnology Information www.ncbi.nlm.nih.gov/) may also be used. When using any of the aforementioned algorithms, the default parameters for "Window" length, gap penalty, etc., are used.

[0062] In one embodiment of the present invention, changes in amino acid sequence belong to the modification of peptide's physical and chemical characteristics. For example, amino acid transformation can be performed by improving thermal stability of the peptide, altering substrate specificity, and changing the optimal pH.

[0063] In one embodiment of the present invention, a peptide comprising amino acid sequence of at least one of SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or a peptide fragment of above-mentioned peptides is preferably made of 30 or less amino acids.

[0064] In one embodiment of the present invention, a peptide comprising amino acid sequence of at least one of SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or a peptide fragment of above-mentioned peptides comprises a peptide originages from telomerase, more specifically, telomerase of Homo sapiens.

[0065] The term "amino acid" herein includes not only the 22 standard amino acids that are naturally integrated into peptide but also the D-isomers and transformed amino acids. Therefore, in a specific embodiment of the present invention, a peptide herein includes a peptide having D-amino acids. On the other hand, a peptide may include non-standard amino acids such as those that have been post-translationally modified. Examples of post-translational modification include phosphorylation, glycosylation, acylation (including acetylation, myristorylation, plamitoylation), alkylation, carboxylation, hydroxylation, glycation, biotinylation, ubiquitinylation, transformation in chemical properties (e.g. .beta.-removing deimidation, deamidation) and structural transformation (e.g. formation of disulfide bridge). Also, changes of amino acids are included and the changes of amino acids occur due to chemical reaction during the combination process with crosslinkers for formation of a peptide conjugate.

[0066] A peptide disclosed herein may be a wild-type peptide that has been identified and isolated from natural sources. On the other hand, when compared to peptide fragments of SEQ ID NO: 1, the peptides disclosed herein may be artificial mutants that comprise one or more substituted, deleted and/or inserted amino acids. Amino acid alteration in wild-type polypeptide--not only in artificial mutants--comprises conservative substitution of amino acids that do not influence protein folding and or activation. Examples of conservative substitution belong to the group consisting of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagines), hydrophobic amino acids (leucine, isoleucine, valine and methionine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, and threonine). The amino acid substitutions that do not generally alter the specific activity are known in the art of the present invention. Most common occurred alteration are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, Asp/Gly, and the opposite alterations. Another example of conservative substitutions are shown in the following table 2.

TABLE-US-00002 TABLE 2 Original Examples of residue Preferable residue amino acid substitution substitution Ala (A) val; leu; ile Val Arg (R) lys; gln; asn Lys Asn (N) gln; his; asp, lys; arg Gln Asp (D) glu; asn Glu Cys (C) ser; ala Ser Gln (Q) asn; glu Asn Glu (E) asp; gln Asp Gly (G) ala Ala His (H) asn; gln; lys; arg Arg Ile (I) leu; val; met; ala; phe; norleucine Leu Leu (L) norleucine; ile; val; met; ala; phe Ile Lys (K) arg; gln; asn Arg Met (M) leu; phe; ile Leu Phe (F) leu; val; ile; ala; tyr Tyr Pro (P) ala Ala Ser (S) thr Thr Thr (T) ser Ser Trp (W) tyr; phe Tyr Tyr (Y) trp; phe; thr; ser Phe Val (V) ile; leu; met; phe; ala; norleucine Leu

[0067] The substantial transformation of the biological properties of peptides are performed by selecting significantly different substitution in the following efficacies: (a) the efficacy in maintaining the structure of the polypeptide backbone in the area of substitution, such as sheet or helical three-dimensional structures, (b) the efficacy in maintaining electrical charge or hydrophobicity of the molecule in the target area, or (c) the efficacy of maintaining the bulk of the side chain. Natural residues are divided into groups by general side chain properties as the following:

(1) hydrophobicity: Norleucine, met, ala, val, leu, ile; (2) neutral hydrophilicity: cys, ser, thr; (3) acidity: asp, glu; (4) basicity: asn, gln, his, lys arg; (5) residue that affects chain orientation: gly, pro; and (6) aromaticity: trp, tyr, phe.

[0068] Non-conservative substitutions may be performed by exchanging a member of the above classes to that of a different class. Any cysteine residues that are not related in maintaining the proper three-dimensional structure of the peptide can typically be substituted into serine, thus increasing the oxidative stability of the molecule and preventing improper crosslinkage. Conversely, improvement of stability can be achieved by adding cysteine bond(s) to the peptide.

[0069] Altered types of amino acids variants of peptides are those that antibody glycosylation pattern changed. The term "change" herein relates to deletion of carbohydrate residues and/or addition of at least one glycosylated residues that do not exist within a peptide.

[0070] Glycosylation in peptides are typically N-connected or O-connected. The term "N-connected" herein relates to that carbohydrate residues are attached to the side chain of asparagine residues. As tripeptide sequences, asparagine-X-serine and asparagine-X-threonine (where the X is any amino acid except proline) are the recognition sequence for attaching carbohydrate residue enzymatically to the side chain of asparagine. Therefore, with the presence of one of these tripeptide sequences in a polypeptide, the potential glycosylation sites are created. "O-connected glycosylation" means attaching one of sugar N-acetylgalactosamine, galactose, or xylose to hydroxyl amino acids. The hydroxyl amino acids are most typically serine or threonine, but 5-hydroxyproline or 5-hydroxylysine can be used.

[0071] Addition of glycosylation site to a peptide is conveniently performed by changing amino acid sequence to contain tripeptide sequence mentioned above (for N-linked glycosylation sites). These changes may be made by addition of at least one serine or theronine residues to the first antibody sequence, or by substitution with those residues (for O-linked glycosylation sites).

[0072] In one embodiment of the present invention, a polynucleotide is a nucleic acid molecule that can be spontaneous or artificial DNA or RNA molecules, either single-stranded or double-stranded. The nucleic acid molecule can be one or more nucleic acids of same type (for example, having a same nucleotide sequence) or nucleic acids of different types. The nucleic acid molecules comprise one or more DNA, cDNA, decoy DNA, RNA, siRNA, miRNA shRNA, stRNA, snoRNA, snRNA PNA, antisense oligomer, plasmid and other modified nucleic acids, but not limited to those.

[0073] A HMGB1 protein is known as a cytokine. It first undergoes acetylation and translocation to cytoplasm by external stimulation. Then it is secreted out of the cell, therefore serving the role of inflammation-causing cytokine. Because when one has an inflammation due to such activity, HMGB1 protein is secreted out of the cell, and patients with inflammatory diseases such as Churg strauss syndrome, rheumatoid arthritis and Sjogren's syndrome will present with elevated serum levels of HMGB1. Hence, if nucleus contains large amount of HMGB1 even when there is a stimulus that causes inflammation, it is suggestive of the fact that HMGB1 is not being secreted out of the cell, which means inflammation is being suppressed.

[0074] In one embodiment of the present invention, when treated a cell with a peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, a peptide having above 80% homology of amino acid sequence with above-mentioned sequences, or a fragment of the above-mentioned peptides, amount of HMGB1 within the nucleus increases. This represents that the peptides mentioned above have excellent inflammation preventive or suppressive effects.

[0075] Also, in specific embodiments of the present invention, a peptide comprising any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, a peptide having above 80% homology of amino acid sequence with above-mentioned sequences, or a fragment of the above-mentioned peptides, has an advantage in that it has high feasibility due to its low toxicity within a cell.

[0076] In the present invention, an "inflammatory disease" is a broad indication that refers to any disease that designates inflammation as a main cause or inflammation caused by disease. Specifically, an inflammatory disease includes (1) general or localized inflammatory disease (for example, allergies; immune-complex disease; hayfever; hypersensitive shock; endotoxin shock; cachexia, hyperthermia; granulomatosis; or sarcoidosis); (2) gastro-intestinal related diseases (for example, appendicitis; gastric ulcer; duodenal ulcer; peritonitis; pancreatitis; ulcerative, acute, or ischemic colitis; cholangitis; cholecystitis, steatorrhea, hepatitis, Crone's disease; or Whipple's Disease); (3) dermal related diseases (for example, psoriasis; burns; sunburns; dermatitis; Urticarial warts or wheal); (4) vascular related diseases (for example, angiitis; vasculitis; endocarditis; arteritis; atherosclerosis; thrombophlebitis; pericarditis; congestive heart failure; myocarditis; myocardial ischemia; periarteritis nodosa; recurrent stenosis; Buerger's disease; or rheumatic fever); (5) respiratory diseases (for example, asthma; epiglottitis; bronchitis; emphysema; rhinitis; cystic fibrosis; interstitial pneumonitis; COPD (chronic obstructive pulmonary disease); adult respiratory distress syndrome; coniosis; alveolitis; bronchiolitis; pharyngitis; pleurisy; or sinusitis); (6) bone, joint, muscle and connective tissue related diseases (for example, eosinophilic granuloma; arthritis; arthralgia; osteomyelitis; dermatomyositis; fasciitis; Paget's disease; gout; periodontal disease; rheumatoid arthritis; myasthenia gravis; ankylosing spondylitis; or synovitis); (7) urogenital disorders (for example, epididymitis; vaginitis; prostatitis; or urethritis); (8) central or peripheral nervous system related diseases (for example, Alzheimer's disease; meningitis; encephalitis; multiple sclerosis; cerebral infarction; cerebral embolism; Guillain-Barre syndrome; neuritis; neuralgia; spinal cord injury; paralysis; or uveitis); (9) virus (for example, influenza; respiratory syncytial virus; HIV; hepatitis B; hepatitis C; or herpes virus), infectious disease (for example, Dengue fever; or septicemia), fungal infection (for example, candidiasis); or bacterial, parasitic, and similar microbial infection (for example, disseminated bacteremia; malaria; onchocerciasis; or amebiasis); (10) autoimmune disease (for example, thyroiditis; lupus; Goodpasture's syndrome; allograft rejection; graft versus host disease; or diabetes); and (11) cancer or tumor disease (for example, Hodgkin's disease), but not limited to those.

[0077] Treating the inflammatory component of such diseases has been a major goal of the global pharmaceutical industry for a number of decades, and a wide variety of useful treatments have been developed. Examples include the corticosteroids (a range of natural, semisynthetic and synthetic agents designed to mimic the effect of cortisol, including prednisolone, methylprednisolone, dexamethasone, betamethasone, fluticasone and so forth), cyclooxygenase inhibitors (both non-selective or cox-1 selective, such as indomethacin, sulfasalzine and aspirin, and more recently cox-2 selective, such as celecoxib), leukotriene blockers (such as monteleukast) and anti-TNFs (such as modified monoclonal neutralising antibodies, including infliximab (Remicade.TM.) and adalimumab (Humira.TM.), TNF receptor fusion proteins, such as etanercept (Enbrel.TM.), as well as small molecule TNF-.alpha. synthesis inhibitors like thalidomide).

[0078] In one embodiment of the present invention, an anti-inflammatory composition comprising a peptide as an active ingredient is provided. The peptide comprises at least one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology with above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides.

[0079] In one embodiment of the present invention, the anti-inflammatory composition may contain 0.1 .mu.g/mg to 1 mg/mg, specifically 1 .mu.g/mg to 0.5 mg/mg, more specifically 10 .mu.g/mg to 0.1 mg/mg of a peptide comprising of amino acid sequence of at least one of SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or peptide fragment of above-mentioned peptides. When the peptide is contained in the above mentioned range, all the safety and stability of the composition may be satisfied and appropriate in terms of cost-effectiveness.

[0080] In one embodiment of the present invention, the composition may have application with all animals including human, dog, chicken, pig, cow, sheep, guinea pig, and monkey.

[0081] In one embodiment of the present invention, the medical composition for the use of treatment or prophylaxis of inflammatory disease with an active ingredient that is comprised of a peptide consisting of an amino acid sequence from SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or peptide fragment of SEQ ID NO:1, is provided. In one embodiment of the present invention, the pharmaceutical composition may be administered through oral, rectal, transdermal, intravenous, intramuscular, intraperitoneal, in the bone marrow, epidural or subcutaneous means.

[0082] Forms of oral administration may be, but not limited to, tablets, pills, soft or hard capsules, granules, powders, solution, or emulsion. Forms of non-oral administration may be, but not limited to, injections, drips, lotions, ointments, gels, creams, suspensions, emulsions, suppository, patch, or spray.

[0083] In one embodiment of the present invention, the pharmaceutical composition, if necessary, may contain additives, such as diluents, excipients, lubricants, binders, disintegrants, buffers, dispersants, surfactants, coloring agents, aromatics or sweeteners. In one embodiment of the present invention, the pharmaceutical composition can be manufactured by conventional methods of the industry in the art.

[0084] In one embodiment of the present invention, the active ingredient of the medical composition may vary according to the patient's age, sex, weight, pathology and state, administration route, or prescriber's judgment. Dosage based on these factors is determined within levels of those skilled in the art, and the daily dose for example may be, but not limited to, 0.1 .mu.g/kg/day to 1 g/kg/day, specifically 1 .mu.g/kg/day to 10 mg/kg/day, more specifically 10 .mu.g/kg/day to 1 mg/kg/day, more specifically 50 .mu.g/kg/day to 100 .mu.g/kg/day. In one embodiment of the present invention, the pharmaceutical composition may be administered, but not limited to, 1 to 3 times a day.

[0085] In one embodiment of the present invention, a skin external composition for improvement or prevention of skin inflammation is provided. The skin external composition may contain an active ingredient that is a peptide comprising of an amino acid sequence from SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or peptide fragment of above-mentioned peptides.

[0086] In another embodiment of the present invention, a cosmetic composition for improvement or prevention of skin inflammation is provided. The cosmetic composition may contain an active ingredient that is a peptide comprising of an amino acid sequence from SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or peptide fragment of above-mentioned peptides.

[0087] In one embodiment of the present invention, external application composition or cosmetic composition may be provided in all forms appropriate for topical applications. For example, forms can be provided as solutions, emulsions obtained by dispersion of oil phase in water, emulsion obtained by dispersion of water in oil phase, suspension, solid, gel, powder, paste, foam or aerosol. These forms can be manufactured by conventional methods of the industry in the art.

[0088] In one embodiment of the present invention, the cosmetic composition may include, within levels that will not harm the main effect, other ingredients that can desirably increase the main effect. In one embodiment of the present invention, the cosmetic composition may additionally include moisturizer, emollient agents, surfactants, UV absorbers, preservatives, fungicides, antioxidants, pH adjusting agent, organic or inorganic pigments, aromatics, cooling agent or antiperspirant. The formulation ratio of the above-mentioned ingredients can be decided by those skilled in the art within levels that will not harm the purpose and the effects of the present invention, and the formulation ratio based on total weight of the cosmetic composition can be 0.01 to 5% by weight, specifically 0.01 to 3% by weight.

[0089] In one embodiment of the present invention, a food composition for inflammation prevention or suppression is provided. The food composition may contain with an active ingredient that is a peptide comprising of an amino acid sequence from SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or peptide fragment of above-mentioned peptides.

[0090] In one embodiment of the present invention, food composition is not limited to forms, but for example may be granules, powder, liquid, and solid forms. Each form can be formed with ingredients commonly used in the industry appropriately chosen by those skilled in the art, in addition to the active ingredient, and can increase the effect with other ingredients.

[0091] Decision for dosage on the above-mentioned active ingredient is within the level of those skilled in the art, and daily dosage for example may be 1 .mu.g/kg/day to 10 mg/kg/day, more specifically 10 .mu.g/kg/day to 1 mg/kg/day, more specifically 50 .mu.g/kg/day to 100 .mu.g/kg/day, but not limited to these numbers and can vary according to age, health status, complications and other various factors.

[0092] In one embodiment of the present invention, a use of prevention or treatment of inflammatory disease with a peptide comprising of an amino acid sequence from SEQ ID NO: 1 to SEQ ID NO: 161, a peptide comprising of amino acid sequence above 80% homology with above-mentioned sequences, or peptide fragment of above-mentioned peptides, is provided.

[0093] In one embodiment of the present invention, the method of prevention or treatment of inflammatory disease with applying peptides mentioned above in patients is provided.

[0094] In one embodiment of the present invention, a kit for prophylaxis or treatment of inflammatory diseases is provided. The kit may contain: a peptide with anti-inflammatory activity or a composition comprising of the peptide, wherein the peptide comprises any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161, the peptide has above 80% homology with above-mentioned sequences, or the peptide is a fragment of the above-mentioned peptides; and instructions including at least one of administration dose, administration route, administration frequency, and indication of the peptide or composition.

[0095] The terms used herein is intended to be used to describe the embodiments, not to limit the present invention. Terms without numbers in front are not to limit the quantity but to show that there may be more than one thing of the term used. The term "including", "having", "consisting", and "comprising" shall be interpreted openly (i.e. "including but not limited to").

[0096] Mention of range of numbers is used instead of stating separate numbers within the range, so unless it is explicitly stated, each number can be read as separate numbers integrated herein. The end values of all ranges are included in the range and can be combined independently.

[0097] Unless otherwise noted or clearly contradicting in context, all methods mentioned herein can be performed in the proper order. The use of any one embodiment and all embodiment, or exemplary language (e.g., that use "like .about."), unless included in the claims, is used to more clearly describe the present invention, not to limit the scope of the present invention. Any language herein outside of the claims should not be interpreted as a necessity of the present invention. Unless defined otherwise, technical and scientific terms used herein have meaning normally understood by a person skilled in the art that the present invention belongs to.

[0098] The preferred embodiments of the present invention are the best mode known to the inventors to perform the present invention. It may become clear to those skilled in the art after reading the statements ahead of the variations in the preferred embodiments. The present inventors hope that those skilled in the art can use the variations adequately and present invention be conducted in other ways than listed herein. Thus, the present invention, as allowed by the patent law, includes equivalents, and variations thereof, of the key points of the invention stated in the appended claims. In addition, all possible variations within any combination of the above-mentioned components are included in the present invention, unless explicitly stated otherwise or contradicting in context. Although the present invention is described and shown by exemplary embodiments, those skilled in the art will understand well that there can be various changes in the form and details without departing from the spirit of the invention and range, defined by the claims below.

[0099] Tumor necrosis factor (TNF), particularly TNF-.alpha., is known to be released from inflammatory cells and cause various cytotoxic reactions, immunological reactions and inflammatory reactions. TNF-.alpha. is known to be involved in the occurrence and prolongation of many inflammatory and autoimmune diseases and further cause serious septicemia and septic shock when it is released into the blood and acts systemically. Because TNF-.alpha. is a factor associated widely with the immune system of a living body, the development of agents inhibiting TNF-.alpha. is actively carried out. TNF-.alpha. is biosynthesized in an inactive form and becomes an active form by being cleaved by protease; the enzyme responsible for the activation is called a tumor necrosis factor-converting enzyme (TACE). Thus, a substance inhibiting this TACE can treat, improve, or prevent diseases, pathologic conditions, abnormal conditions, troubles, adverse symptoms and the like ascribed to TNF-.alpha..

[0100] High-mobility group box 1(HMGB1) protein exists in high concentrations in thymus, lymph nodes, testes, and in fetal liver, and with exception to liver and brain cells, usually exists inside of the nucleus. The said HMGB1 protein has 3 domains consisting of A-box, B-box, and C-terminal.

[0101] It was reported by Tracey et al., 1999 that HMGB1 protein has a role as a cytokine which induces inflammation, and the mechanism of said HMGB1's inflammation induction is by an external stimulus causing acetylation of HMGB1 which then moves from the nucleus into the cytoplasm. Afterward, it is known to be secreted out of the cell, or secreted out from the cell in necrosis. (Bonaldi T et al., EMBO J, (22)5551-60, 2003).

[0102] The invention is further described by the figures, the following examples and experiments, which are solely for the purpose of illustrating specific embodiments of this invention, and are not to be construed as limiting the scope of the invention in any way.

Example 1

Synthesis of PEP-1 and Measurement of Anti-Inflammatory Activities of PEP-1 (SEQ ID NO: 1)

Experiment 1

Synthesis of PEP-1 (SEQ ID NO: 1)

[0103] A peptide comprised of 16 amino acids with the chemical structure 1 as below having the sequence SEQ ID: 1 (PEP-1) derived from human telomerase was synthesized:

##STR00001##

[0104] SEQ ID NO: 1 (PEP-1) was synthesized according to the existing method of solid phase peptide synthesis. In detail, the peptides were synthesized by coupling each amino acid from C-terminus through Fmoc solid phase peptide synthesis, SPPS, using ASP48S (Peptron, Inc., Daejeon ROK). Those peptides with their first amino acid at the C-terminus being attached to resin were used as follows:

NH.sub.2-Lys(Boc)-2-chloro-Trityl Resin NH.sub.2--Ala-2-chloro-Trityl Resin NH.sub.2--Arg(Pbf)-2-chloro-Trityl Resin

[0105] All the amino acid materials to synthesize the peptide were protected by Fmoc at the N-terminus, and the amino acid residues were protected by Trt, Boc, t-Bu (t-butylester), Pbf (2,2,4,6,7-pentamethyl dihydro-benzofuran-5-sulfonyl) that can be dissolved in acid. Such as:

Fmoc-Ala-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Pro-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Gln(Trt)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Met-OH, Fmoc-Asn(Trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Ahx-OH, Trt-Mercaptoacetic acid.

[0106] HBTU[2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetamethylaminium hexafluorophosphate]/HOBt [N-Hydroxybenzotriazole]/NMM [4-Methylmorpholine] were used as the coupling reagents. In 20% of DMF, piperidine was used to remove Fmoc. In order to remove the protection from residue or to separate the synthesized peptide from Resin, cleavage cocktail [TFA (trifluoroacetic acid)/TIS (triisopropylsilane)/EDT (ethanedithiol)/H.sub.2O=92.5/2.5/2.5/2.5] was used.

[0107] Synthesized the peptide by using the solid phase scaffold combined to starting amino acid with the amino acid protection, reacting the corresponding amino acids separately, washing with solvent and deprotected, and repeating the process. After cutting off the synthesized peptide from the resin, it was purified by HPLC and verify for synthesis by MS, and then freeze-dried.

[0108] Specific synthesis process of PEP 1 is described by the following. [0109] 1) Coupling

[0110] Melted the amino acid (8 equivalent) protected with NH.sub.2-Lys(Boc)-2-chloro-Trityl Resin, and coupling agent HBTU(8 equiv.)/HOBt(8equiv.)/NMM(16 equiv.) and added to DMF, then let react in room temperature for 2 hours, then washed with DMF, MeOH, and DMF in that order. [0111] 2) Fmoc deprotection

[0112] Added 20% piperidine in DMF and reacted in room temperature for 5 minutes 2 times, then washed with DMF, MeOH, and DMF in that order. [0113] 3) Make the basic framework of peptide by repeating reactions 1 and 2 repeatedly. [0114] 4) Cleavage: Add Cleavage Cocktail to the completely synthesized peptide and separated the peptide from the resin. [0115] 5) Add cooling diethyl ether into obtained mixture, and then centrifugation is used to precipitate gathered peptide. [0116] 6) After purification by Prep-HPLC, check the molecular weight by LC/MS and lyophilize to produce in powder form.

Experiment 2

Anti-Inflammatory Activity Measurement of PEP 1

Cell Lines Culture

[0117] Raw 264.7macrophage cell (KCBL, 40071) from Korea Cell Bank was maintained in Dulbecco's modified Eagle's medium (DMEM; PAA, Austria) containing 10% fetal bovine serum (FBS; Gibco Laboratories), 100 unit/mL of streptomycin, and penicillin (Gibco Laboratories) at 37.degree. C. with 5% CO.sub.2. Raw264.7 cells were seeded into a 96-well plate at a density of 1.times.10.sup.6 cells/mL and incubated overnight.

[0118] On the following day, the medium was replaced with fresh medium and 5 .mu.g/mL of peptide (obtained as described in Experiment example 1) was added to the cells. After 30 min incubation of cells with the peptide 50 .mu.L of LPS (to a final concentration of 1 .mu.g/mL) was added, and cells were incubated for additional 24 hr. The experimental sample with the induction of inflammatory response was treated with 1 .mu.g/mL mL lipopolysaccharide (LPS; Sigma, USA) and control sample was treated with phosphate buffered saline (PBS; pH 7.2). The supernatant samples from each condition was collected in eppendorf tubes and subjected to further analysis.

Experiment 2-1

NO Level Analysis

[0119] The level of nitric oxide (NO) was measured in Raw 264.7 cell (1.times.10.sup.6 cell/ml) using Griess reagent system (Promega, USA). Culture medium of 50 .mu.l was added to a 96-well plate and Griess reagent I (NED) solution and Griess reagent II (Sulfaniliamide solution) are added in the same amount. After 10 min incubation of cells with the reagents, the optical density at 540 nm was measured within 30 min using a microplate reader (Molecular Devices, USA). The concentration of NO was calculated by using a standard curve (0.about.100 .mu.M) of sodium nitrite.

[0120] As shown in Table 3 below, stimulation of cells with LPS increased the expression of NO, but in co-treatment with LPS and Pep1, the expression level of NO mentioned above decreased. NO is produced during inflammation, and the result showing Pep1 reduced NO level to 65% of the control strongly support the anti-inflammatory effect of Pep1.

TABLE-US-00003 TABLE 3 The measurement of anti-inflammatory effect of human telomerase derived PEP 1 NO Expression Decreased Level of NO Expression Test sample control (%) Level (%) PBS 0 -- LPS 1 .mu.g/mL PBS 100 0 PEP 1 (0.5 .mu.g/mL) 35 65

Experiment 2-2

Analysis of Cytokine Inhibitory Effect

[0121] To investigate the effect of PEP1 on inhibiting pro-inflammatory cytokine production RAW 264.7 cell were pre-treated with PEP 1 at a concentration of 5 .mu.g/mL challenged with LPS at a concentration of 1 .mu.g/mL, and cells were further incubated for 24 hr. The supernatant samples containing cell culture medium was collected and analyzed for the cytokine levels using ELISA kits (eBioscience, San Diego).

[0122] 96 wells plates were coated with 100 .mu.l of capture antibodies (diluted in coating buffer to the concentration recommended by manufacturer's protocol) overnight at 4.degree. C. Then, after washing the plates 5 times, 200 .mu.L of assay diluents was added to each well and incubated for 1 hr at room temperature for blocking. After washing each well with wash buffer five times, cell culture sample or each cytokine standard protein sample was diluted and 100 .mu.l of each added into each well. The plate containing samples were incubated overnight at 4.degree. C. Then, after washing the plate five times with the wash buffer, 100 .mu.l of secondary antibody conjugated to avidin was added and incubated for 1 hr at room temperature.

[0123] Following incubation with the secondary antibody, the plate was washed five times and incubated with 100 .mu.l of avidin-HRP (BD Bioscience) for 30 min at room temperature. After washing the plate seven times, 100 .mu.l of TMB solution (Pierce) was added and incubated for 15 min at room temperature. The reaction was stopped by adding 50 .mu.l of 2N H.sub.2SO.sub.4 in each well The optical density at 450 nm was measured using a microplate reader. Statistical analysis was performed by variance analysis using ANOVA procedure of SPSS program, and verified the significance between analyses using Duncan's multiple range test.

Experiment 2-3

IL-6 Secretion Measurement

[0124] As shown in Table 4 below, treatment with LPS alone increased the cytokine IL-6 (interleukin-6) secretion. However, co-treatment with LPS and PEP-1 showed a decrease in the level of the pro-inflammatory cytokine IL-6 secretion. More importantly, after the treatment with PEP-1, the level of pro-inflammatory cytokine secretion decreased by more than 70%, which indicates a robust anti-inflammatory effect of Pep1.

TABLE-US-00004 TABLE 4 Cytokine IL-6 production inhibition by PEP-1 cytokine IL-6 production Test sample % of control inhibition % PBS 0 -- LPS 1 .mu.g/ml PBS 100 0 PEP 1 (5 .mu.g/ml) 28 72

Experiment 2-4

HMGB1, TNF-.alpha., COX-2 Expression Inhibition

[0125] Protein expression level was determined by Western blot analysis. Cells grown in PEP-1 containing medium were washed with PBS, treated with 0.05% trypsin-EDTA, and collected by centrifugation. The collected cells were dissolved in an appropriate volume of lysis buffer. Intracellular debris was pelleted by centrifugation, and equal amount of protein from each sample was separated by SDS-polyacrylamide gel electrophoresis. The separated protein was transferred to nitrocellulose membrane (Schleicherand Schuell, Keene, N. H., USA), then was tested for the antibody specific for each protein.. The membrane was incubated with ECL (enhanced chemiluminoesence) solution (Amersham Life Science Corp., Arlington Heights, Ill., USA), exposed to X-ray, and the level of protein expression was analyzed according to the exposure level shown on the X-ray film.

[0126] Western blot analysis was performed to determine the inhibitory effect of Ppep1 on the cytokine protein expression. As shown in Table 5 below, stimulation of cells with LPS increased the expression of cytokines; HMGB1, TNF-.alpha. and COX. However, if cells were treated with both LPS and Pep1, the expression level of pro-inflammatory cytokines mentioned above decreased. The result showing the treatment with Pep1 decreased pro-inflammatory cytokine levels by more than 70% provide strong evidence supporting the anti-inflammatory effect of Pep1.

TABLE-US-00005 TABLE 5 The measurement of inhibitory effect of Pep1 on pro-inflammatory cytokine expression level. Cytokine Expression Level (band intensity) % of control Test sample HMGB1 TNF-.alpha. COX-2 PBS -- -- -- LPS 1 .mu.g/ml PBS 100 100 100 PEP 1 30 25 22 (5 .mu.g/ml)

Example 2

TNF-.alpha. Inhibitory Effect of PEP RIA Series (Sequence No. 1 to 161) Peptides

[0127] Based on the results from Example 1 in which the SEQ ID NO: 1 (PEP1) has the TNF-.alpha. inhibitory effect, experiment using peptides SEQ ID NO: 1 to 161 were carried out to confirm their TNF-.alpha. inhibitory effect. The synthesis of peptides SEQ ID NO: 1 to 161 used the same method mentioned above in Example 1 (method used for synthesis of PEP1), but the amino acids added were different.

Experiment 1

Cell Culture

[0128] PBMC (peripheral blood mononuclear cells) layer was separated from blood samples (50 ml) collected in healthy subjects using Biocoll Separating Solution (Biochrom AG, Berlin, Germany). The collected PBMC were enriched in RPMI 1640 medium containing 20% human serum for 30 mins, and then transferred to 100-mm polystyeren cell culture plate coated with human serum for incubation for 2 hrs at 37.degree. C., 5% CO.sup.2 incubator. Monocytes were detached from the bottom of the plate using cold PBS, and incubated to reach the number of 1.times.10.sup.5 cell/well in 96-well plate with RPMI 1640 medium (supplemented with penicillin-streptomycin; 100 mg/ml, human serum; 20%) over night.

Experiment 2

Analysis of TNF-.alpha. Inhibitory Effect in PBMC-Derived Monocyte

[0129] ELISA was performed to find out how the peptides of the PEP RIA series influence TNF-.alpha. level. PBMC-derived monocytes were incubated to reach the number of 1.times.10.sup.5 cellsper well in a 96-well plate and then treated with LPS (lipopolysaccharide; 10 ng/ml, Sigma) for 2 hours. To the monocytes that were washed three times with PBS, OPTI-MEM culture medium was added to induce cell starvation for an hour, 4 .mu.M of the peptide was taken out and incubated for 2 hours. There were three negative control groups. The first group was not treated with anything. The second group that was treated with estrogen (in this experiment, estradiol was used as a kind of estrogen). The third group was treated with LPS (10 ng/ml) or with LPS (10 ng/ml) as well as estrogen (20 nM). PEP1 that was confirmed to have TNF-.alpha. inhibiting activity was used as a positive control to measure TNF-.alpha. inhibiting activity. After incubation, TNF-.alpha. was measured by following the ELISA kit manual (R&D, Minneapolis, Minn., USA). The details of quantification method can be found in Experiment 2.2 of Example 1.

[0130] Using the method stated above, Peptides with TNF-.alpha. inhibiting effect were screened. PBMC-derived monocytes were stimulated with LPS (10 ng/ml), which is endotoxin, for 2 hours and were induced to starve by adding OPTI-MEM for 1 hour. After that, 4 .mu.M of 161 peptides were treated and incubated for 2 hrs. The amount of TNF-.alpha. in the cell culture medium was measured using ELISA, and the peptides with TNF-.alpha. inhibiting effect were screened by comparing to the negative and positive controls (FIG. 1 to FIG. 16).

[0131] The followings are the peptides that showed TNF-.alpha. inhibiting effect when compared to the control group that was treated with only LPS: SEQ ID NO: 1 to SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 14 to 21, SEQ ID NO: 23 to SEQ ID NO: 37, SEQ ID NO: 39 to SEQ ID NO: 44, SEQ ID NO: 47 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 61, SEQ ID NO: 63 to SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 99 to SEQ ID NO: 104, SEQ ID NO: 107 to SEQ ID NO: 109, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 120 to SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 129 to SEQ ID NO: 133, SEQ ID NO: 142 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, and SEQ ID NO: 155 to SEQ ID NO: 159.

[0132] Also, the followings are the peptides that showed TNF-.alpha. inhibiting effect when compared to the group treated with LPS and estrogen: SEQ ID NO: 15 to SEQ ID NO: 18, SEQ ID NO: 23 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31 to SEQ ID NO: 34, SEQ ID NO: 39 to SEQ ID NO: 41, SEQ ID NO: 47, SEQ ID NO:48, SEQ ID NO: 51 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 58, SEQ ID NO: 61, SEQ ID NO: 65 to SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 73 to SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 87, SEQ ID NO: 8=90 to SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 99, SEQ ID NO: 101 to SEQ ID NO: 104, SEQ ID NO: 107 to SEQ ID NO: 109, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 129 to SEQ ID NO: 132, SEQ ID NO: 142 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, and SEQ ID NO: 157 to SEQ ID NO: 159.

Experiment 3

Analysis of Peptides Affecting TNF-.alpha. Level in THP1 Cell Line

[0133] Experiment was carried out using THP-1 cell line (American Type Culture Collection (ATCC), Manassas, Va., USA) which is human acute monocytic leukemia.

[0134] THP-1 cells were incubated to reach the number of 1.times.10.sup.5 cellsper well in a 96-well plate with RPMI 1640 medium for 24 hrs, followed by addition of 100 .mu.M of PMA (phorbol 12-myristate 13-acetate) for the differentiation into macrophage. After differentiation of THP-1 into macrophage by PMA for a day, LPS was treated for 2 hrs and washed off. Starvation for an hour and PEP1 treatment followed.

[0135] THP-1 cell differentiated by PMA was treated with LPS (lipopolysaccharide; 10 ng/ml, Sigma) for 2 hours, followed by 2 times washes with PBS. To the cells, OPTI-MEM culture medium was added to induce cell starvation for an hour, and 1 .mu.M of 161 peptides was taken out and incubated for an hour. After incubation, TNF-.alpha. level was measured by using the ELISA kit and the peptides which reduce the TNF-.alpha. level were screened (FIG. 17 to FIG. 35).

[0136] As a result, peptide SEQ ID NO: 1 to SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 32 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 60, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72 to SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 99 to SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 127 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 151 and SEQ ID NO: 153 to SEQ ID NO: 161 appeared to reduce the TNF-.alpha. level compared to control group treated only with LPS.

[0137] In addition, SEQ ID NO: 1 to SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17 to SEQ ID NO: 23, SEQ ID NO: 25 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 33 to SEQ ID NO: 43, SEQ ID NO: 156, SEQ ID NO: 157 and SEQ ID NO: 159 were selected as peptides which reduce the expression level of TNF-.alpha. compared to that of group treated with LPS and estrogen.

Example 3

Effects of Peptides (SEQ ID No:1 to 161) on Inflammation by Amyloid-.beta. Protein

[0138] HMGB1 first undergoes acetylation and translocation to cytoplasm by external stimulation. Then it is secreted out of the cell, therefore serving the role of inflammation-causing cytokine. Because when one has an inflammation due to such activity, HMGB1 protein is secreted from the cell, and patients with inflammatory diseases such as Churg strauss syndrome, rheumatoid arthritis and Sjogren's syndrome will present with elevated serum levels of HMGB1. Hence, if nucleus contains large amount of HMGB1 even when there is a stimulus that causes inflammation, it is suggestive of the fact that HMGB1 is not being secreted out of the cell, which means inflammation is being suppressed.

Experiment 1

Cell Culture

[0139] Undifferentiated PC12 cells (ATCC, Rockville, Md., USA) were maintained in ogarithmic-phase growth on poly-l-lysine (Sigma, Saint Louis, Mo., USA)--precoated 100 mm dishes (Corning, Pa., USA) in RPMI 1640 medium (GIBCO, Grand Island, N.Y., USA) containing 10% heat-inactivated horse serum, 5% heat-inactivated fetal bovine serum, 100 units/ml penicillin, and 100 g/ml streptomycin. Cultures were incubated at 37.degree. C. in a humidified atmosphere with 5% CO.sup.2. The cultures were grown to 50% confluence and were harvested in Ca.sup.2+/Mg.sup.2+-free Hank's balanced salt solution containing 1 mM EDTA. Cells were plated at a density of 1.times.10.sup.6 cells/100 mm dish and incubated for 24 hrs. For neuronal differentiation, PC12 cells were serum-starved for 12 hrs (RPMI1640 medium containing 100 units/ml penicillin and 100 g/ml streptomycin without horse serum or fetal bovine serum); thereafter, the cells were maintained in serum-free medium. After two days the medium was replaced with fresh serum-free medium. On day three, NGF (50 ng/ml, Sigma, Saint Louis, Mo., USA) was added to the medium, and the cultures were maintained for an additional three days. After differentiation, nPC12 cells were incubated with 20 .mu.M amyloid-.beta. with several concentrations of the peptides [0 (control), 1, 10, and 50 .mu.M] for 48 hrs.

Experiment 2

Western Blot Analysis

[0140] Levels of HMGB1 were analyzed by western blotting. Briefly, 5.times.10.sup.6 cells were washed twice in cold PBS, incubated for 10 min on ice in lysis buffer [50 mM Tris (pH 8.0), 150 mM NaCl, 0.02% sodium azide, 0.2% SDS, 100 .mu.g/ml phenyl methyl sulfonyl fluoride (PMSF), 50 .mu.l/ml aprotinin, 1% Igepal 630, 100 mM NaF, 0.5% sodium deoxy choate, 0.5 mM EDTA, 0.1 mM EGTA]; unbroken cells and nuclei were pelleted by centrifugation for 10 min at 2000.times.g and the lysates were cleared by centrifugation at 10,000.times.g. The antibodies used were: anti-HMGB1 (1:1000, Cell Signaling, Beverly, Mass., USA) and anti-.beta.-tubulin (1:1000, Cell Signaling, Beverly, Mass., USA). The membranes were washed with Tris-buffered saline containing 0.05% Tween-20 (TBST), and then processed using HRP-conjugated anti-rabbit antibody (Amersham Pharmacia Biotech, Piscataway, N.J., USA) followed by ECL detection (Amersham Pharmacia Biotech,). The blots were quantified with an image analyzer (GE Healthcare, ImageQuant LAS 4000).

[0141] As a result of the western blot analysis, peptides showing accumulation of HMGB1 in the cell were selected. FIG. 36 to FIG. 108 are the western blot results of selected peptides. Tubulins in these figures are used for confirming protein expression. The sequences of the selected peptides are as follows:

SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 52, SEQ ID NO: 57, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 91, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 118, SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 129, SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 135, SEQ ID NO: 146, SEQ ID NO: 151, SEQ ID NO: 154, and SEQ ID NO: 156.

Sequence CWU 1

1

163130PRTHomo sapiens 1Ala Leu Phe Ser Val Leu Asn Tyr Glu Arg Ala Arg Arg Pro Gly Leu 1 5 10 15 Leu Gly Ala Ser Val Leu Gly Leu Asp Asp Ile His Arg Ala 20 25 30 215PRTHomo sapiens 2Ser Val Leu Asn Tyr Glu Arg Ala Arg Arg Pro Gly Leu Leu Gly 1 5 10 15 310PRTHomo sapiens 3Gly Leu Leu Gly Ala Ser Val Leu Gly Leu 1 5 10 410PRTHomo sapiens 4Ala Leu Leu Thr Ser Arg Leu Arg Phe Ile 1 5 10 59PRTHomo sapiens 5Arg Pro Ala Leu Leu Thr Ser Arg Leu 1 5 69PRTHomo sapiens 6Arg Leu Thr Ser Arg Val Lys Ala Leu 1 5 715PRTHomo sapiens 7Arg Thr Phe Val Leu Arg Val Arg Ala Gln Asp Pro Pro Pro Glu 1 5 10 15 815PRTHomo sapiens 8Arg Leu Thr Ser Arg Val Lys Ala Leu Phe Ser Val Leu Asn Tyr 1 5 10 15 915PRTHomo sapiens 9Ala Glu Arg Leu Thr Ser Arg Val Lys Ala Leu Phe Ser Val Leu 1 5 10 15 109PRTHomo sapiens 10Tyr Glu Arg Ala Arg Arg Pro Gly Leu 1 5 119PRTHomo sapiens 11Leu Leu Gly Ala Ser Val Leu Gly Leu 1 5 1210PRTHomo sapiens 12Val Leu Gly Leu Asp Asp Ile His Arg Ala 1 5 10 1310PRTHomo sapiens 13Gly Ala Ser Val Leu Gly Leu Asp Asp Ile 1 5 10 1410PRTHomo sapiens 14Ala Leu Phe Ser Val Leu Asn Tyr Glu Arg 1 5 10 1510PRTHomo sapiens 15Ser Val Leu Asn Tyr Glu Arg Ala Arg Arg 1 5 10 1610PRTHomo sapiens 16Ser Val Leu Gly Leu Asp Asp Ile His Arg 1 5 10 1710PRTHomo sapiens 17Phe Ser Val Leu Asn Tyr Glu Arg Ala Arg 1 5 10 1810PRTHomo sapiens 18Asn Tyr Glu Arg Ala Arg Arg Pro Gly Leu 1 5 10 1910PRTHomo sapiens 19Tyr Glu Arg Ala Arg Arg Pro Gly Leu Leu 1 5 10 2010PRTHomo sapiens 20Arg Pro Gly Leu Leu Gly Ala Ser Val Leu 1 5 10 219PRTHomo sapiens 21Glu Arg Ala Arg Arg Pro Gly Leu Leu 1 5 229PRTHomo sapiens 22Val Leu Gly Leu Asp Asp Ile His Arg 1 5 239PRTHomo sapiens 23Ser Val Leu Asn Tyr Glu Arg Ala Arg 1 5 249PRTHomo sapiens 24Val Leu Asn Tyr Glu Arg Ala Arg Arg 1 5 259PRTHomo sapiens 25Ala Arg Arg Pro Gly Leu Leu Gly Ala 1 5 269PRTHomo sapiens 26Pro Gly Leu Leu Gly Ala Ser Val Leu 1 5 279PRTHomo sapiens 27Arg Arg Pro Gly Leu Leu Gly Ala Ser 1 5 289PRTHomo sapiens 28Ala Leu Phe Ser Val Leu Asn Tyr Glu 1 5 299PRTHomo sapiens 29Gly Leu Leu Gly Ala Ser Val Leu Gly 1 5 309PRTHomo sapiens 30Ser Val Leu Gly Leu Asp Asp Ile His 1 5 318PRTHomo sapiens 31Glu Arg Ala Arg Arg Pro Gly Leu 1 5 328PRTHomo sapiens 32Ala Arg Arg Pro Gly Leu Leu Gly 1 5 338PRTHomo sapiens 33Gly Leu Leu Gly Ala Ser Val Leu 1 5 348PRTHomo sapiens 34Arg Ala Arg Arg Pro Gly Leu Leu 1 5 358PRTHomo sapiens 35Leu Gly Ala Ser Val Leu Gly Leu 1 5 3615PRTHomo sapiens 36Val Thr Glu Thr Thr Phe Gln Lys Asn Arg Leu Phe Phe Tyr Arg 1 5 10 15 3715PRTHomo sapiens 37Leu Phe Phe Tyr Arg Lys Ser Val Trp Ser Lys Leu Gln Ser Ile 1 5 10 15 3815PRTHomo sapiens 38Lys Leu Gln Ser Ile Gly Ile Arg Gln His Leu Lys Arg Val Gln 1 5 10 15 3915PRTHomo sapiens 39Glu Ala Glu Val Arg Gln His Arg Glu Ala Arg Pro Ala Leu Leu 1 5 10 15 4015PRTHomo sapiens 40Arg Pro Ala Leu Leu Thr Ser Arg Leu Arg Phe Ile Pro Lys Pro 1 5 10 15 4115PRTHomo sapiens 41Phe Ile Pro Lys Pro Asp Gly Leu Arg Pro Ile Val Asn Met Asp 1 5 10 15 4215PRTHomo sapiens 42Arg Thr Phe Arg Arg Glu Lys Arg Ala Glu Arg Leu Thr Ser Arg 1 5 10 15 4315PRTHomo sapiens 43Leu Asp Asp Ile His Arg Ala Trp Arg Thr Phe Val Leu Arg Val 1 5 10 15 4415PRTHomo sapiens 44Phe Val Leu Arg Val Arg Ala Gln Asp Pro Pro Pro Glu Leu Tyr 1 5 10 15 4515PRTHomo sapiens 45Pro Gln Asp Arg Leu Thr Glu Val Ile Ala Ser Ile Ile Lys Pro 1 5 10 15 4615PRTHomo sapiens 46Lys Ser Val Trp Ser Lys Leu Gln Ser Ile Gly Ile Arg Gln His 1 5 10 15 4716PRTHomo sapiens 47Leu Lys Arg Val Gln Leu Arg Glu Leu Ser Glu Ala Glu Val Arg Gln 1 5 10 15 4820PRTHomo sapiens 48Met Pro Arg Ala Pro Arg Cys Arg Ala Val Arg Ser Leu Leu Arg Ser 1 5 10 15 His Tyr Arg Glu 20 4920PRTHomo sapiens 49Val Leu Pro Leu Ala Thr Phe Val Arg Arg Leu Gly Pro Gln Gly Trp 1 5 10 15 Arg Leu Val Gln 20 5020PRTHomo sapiens 50Arg Gly Asp Pro Ala Ala Phe Arg Ala Leu Val Ala Gln Cys Leu Val 1 5 10 15 Cys Val Pro Trp 20 5120PRTHomo sapiens 51Asp Ala Arg Pro Pro Pro Ala Ala Pro Ser Phe Arg Gln Val Ser Cys 1 5 10 15 Leu Lys Glu Leu 20 5220PRTHomo sapiens 52Val Ala Arg Val Leu Gln Arg Leu Cys Glu Arg Gly Ala Lys Asn Val 1 5 10 15 Leu Ala Phe Gly 20 5320PRTHomo sapiens 53Phe Ala Leu Leu Asp Gly Ala Arg Gly Gly Pro Pro Glu Ala Phe Thr 1 5 10 15 Thr Ser Val Arg 20 5420PRTHomo sapiens 54Ser Tyr Leu Pro Asn Thr Val Thr Asp Ala Leu Arg Gly Ser Gly Ala 1 5 10 15 Trp Gly Leu Leu 20 5520PRTHomo sapiens 55Leu Arg Arg Val Gly Asp Asp Val Leu Val His Leu Leu Ala Arg Cys 1 5 10 15 Ala Leu Phe Val 20 5620PRTHomo sapiens 56Leu Val Ala Pro Ser Cys Ala Tyr Gln Val Cys Gly Pro Pro Leu Tyr 1 5 10 15 Gln Leu Gly Ala 20 5720PRTHomo sapiens 57Ala Thr Gln Ala Arg Pro Pro Pro His Ala Ser Gly Pro Arg Arg Arg 1 5 10 15 Leu Gly Cys Glu 20 5820PRTHomo sapiens 58Arg Ala Trp Asn His Ser Val Arg Glu Ala Gly Val Pro Leu Gly Leu 1 5 10 15 Pro Ala Pro Gly 20 5920PRTHomo sapiens 59Ala Arg Arg Arg Gly Gly Ser Ala Ser Arg Ser Leu Pro Leu Pro Lys 1 5 10 15 Arg Pro Arg Arg 20 6020PRTHomo sapiens 60Gly Ala Ala Pro Glu Pro Glu Arg Thr Pro Val Gly Gln Gly Ser Trp 1 5 10 15 Ala His Pro Gly 20 6120PRTHomo sapiens 61Arg Thr Arg Gly Pro Ser Asp Arg Gly Phe Cys Val Val Ser Pro Ala 1 5 10 15 Arg Pro Ala Glu 20 6220PRTHomo sapiens 62Glu Ala Thr Ser Leu Glu Gly Ala Leu Ser Gly Thr Arg His Ser His 1 5 10 15 Pro Ser Val Gly 20 6320PRTHomo sapiens 63Arg Gln His His Ala Gly Pro Pro Ser Thr Ser Arg Pro Pro Arg Pro 1 5 10 15 Trp Asp Thr Pro 20 6420PRTHomo sapiens 64Cys Pro Pro Val Tyr Ala Glu Thr Lys His Phe Leu Tyr Ser Ser Gly 1 5 10 15 Asp Lys Glu Gln 20 6520PRTHomo sapiens 65Leu Arg Pro Ser Phe Leu Leu Ser Ser Leu Arg Pro Ser Leu Thr Gly 1 5 10 15 Ala Arg Arg Leu 20 6620PRTHomo sapiens 66Val Glu Thr Ile Phe Leu Gly Ser Arg Pro Trp Met Pro Gly Thr Pro 1 5 10 15 Arg Arg Leu Pro 20 6720PRTHomo sapiens 67Arg Leu Pro Gln Arg Tyr Trp Gln Met Arg Pro Leu Phe Leu Glu Leu 1 5 10 15 Leu Gly Asn His 20 6820PRTHomo sapiens 68Ala Gln Cys Pro Tyr Gly Val Leu Leu Lys Thr His Cys Pro Leu Arg 1 5 10 15 Ala Ala Val Thr 20 6920PRTHomo sapiens 69Pro Ala Ala Gly Val Cys Ala Arg Glu Lys Pro Gln Gly Ser Val Ala 1 5 10 15 Ala Pro Glu Glu 20 7020PRTHomo sapiens 70Glu Asp Thr Asp Pro Arg Arg Leu Val Gln Leu Leu Arg Gln His Ser 1 5 10 15 Ser Pro Trp Gln 20 7120PRTHomo sapiens 71Val Tyr Gly Phe Val Arg Ala Cys Leu Arg Arg Leu Val Pro Pro Gly 1 5 10 15 Leu Trp Gly Ser 20 7220PRTHomo sapiens 72Arg His Asn Glu Arg Arg Phe Leu Arg Asn Thr Lys Lys Phe Ile Ser 1 5 10 15 Leu Gly Lys His 20 7320PRTHomo sapiens 73Ala Lys Leu Ser Leu Gln Glu Leu Thr Trp Lys Met Ser Val Arg Asp 1 5 10 15 Cys Ala Trp Leu 20 7420PRTHomo sapiens 74Arg Arg Ser Pro Gly Val Gly Cys Val Pro Ala Ala Glu His Arg Leu 1 5 10 15 Arg Glu Glu Ile 20 7520PRTHomo sapiens 75Leu Ala Lys Phe Leu His Trp Leu Met Ser Val Tyr Val Val Glu Leu 1 5 10 15 Leu Arg Ser Phe 20 7620PRTHomo sapiens 76Phe Tyr Val Thr Glu Thr Thr Phe Gln Lys Asn Arg Leu Phe Phe Tyr 1 5 10 15 Arg Lys Ser Val 20 7720PRTHomo sapiens 77Trp Ser Lys Leu Gln Ser Ile Gly Ile Arg Gln His Leu Lys Arg Val 1 5 10 15 Gln Leu Arg Glu 20 7820PRTHomo sapiens 78Leu Ser Glu Ala Glu Val Arg Gln His Arg Glu Ala Arg Pro Ala Leu 1 5 10 15 Leu Thr Ser Arg 20 7920PRTHomo sapiens 79Leu Arg Phe Ile Pro Lys Pro Asp Gly Leu Arg Pro Ile Val Asn Met 1 5 10 15 Asp Tyr Val Val 20 8020PRTHomo sapiens 80Gly Ala Arg Thr Phe Arg Arg Glu Lys Arg Ala Glu Arg Leu Thr Ser 1 5 10 15 Arg Val Lys Ala 20 8120PRTHomo sapiens 81Leu Phe Ser Val Leu Asn Tyr Glu Arg Ala Arg Arg Pro Gly Leu Leu 1 5 10 15 Gly Ala Ser Val 20 8220PRTHomo sapiens 82Leu Gly Leu Asp Asp Ile His Arg Ala Trp Arg Thr Phe Val Leu Arg 1 5 10 15 Val Arg Ala Gln 20 8320PRTHomo sapiens 83Asp Pro Pro Pro Glu Leu Tyr Phe Val Lys Val Asp Val Thr Gly Ala 1 5 10 15 Tyr Asp Thr Ile 20 8420PRTHomo sapiens 84Pro Gln Asp Arg Leu Thr Glu Val Ile Ala Ser Ile Ile Lys Pro Gln 1 5 10 15 Asn Thr Tyr Cys 20 8520PRTHomo sapiens 85Val Arg Arg Tyr Ala Val Val Gln Lys Ala Ala His Gly His Val Arg 1 5 10 15 Lys Ala Phe Lys 20 8620PRTHomo sapiens 86Ser His Val Ser Thr Leu Thr Asp Leu Gln Pro Tyr Met Arg Gln Phe 1 5 10 15 Val Ala His Leu 20 8720PRTHomo sapiens 87Gln Glu Thr Ser Pro Leu Arg Asp Ala Val Val Ile Glu Gln Ser Ser 1 5 10 15 Ser Leu Asn Glu 20 8820PRTHomo sapiens 88Ala Ser Ser Gly Leu Phe Asp Val Phe Leu Arg Phe Met Cys His His 1 5 10 15 Ala Val Arg Ile 20 8920PRTHomo sapiens 89Arg Gly Lys Ser Tyr Val Gln Cys Gln Gly Ile Pro Gln Gly Ser Ile 1 5 10 15 Leu Ser Thr Leu 20 9020PRTHomo sapiens 90Leu Cys Ser Leu Cys Tyr Gly Asp Met Glu Asn Lys Leu Phe Ala Gly 1 5 10 15 Ile Arg Arg Asp 20 9120PRTHomo sapiens 91Gly Leu Leu Leu Arg Leu Val Asp Asp Phe Leu Leu Val Thr Pro His 1 5 10 15 Leu Thr His Ala 20 9220PRTHomo sapiens 92Lys Thr Phe Leu Arg Thr Leu Val Arg Gly Val Pro Glu Tyr Gly Cys 1 5 10 15 Val Val Asn Leu 20 9320PRTHomo sapiens 93Arg Lys Thr Val Val Asn Phe Pro Val Glu Asp Glu Ala Leu Gly Gly 1 5 10 15 Thr Ala Phe Val 20 9420PRTHomo sapiens 94Gln Met Pro Ala His Gly Leu Phe Pro Trp Cys Gly Leu Leu Leu Asp 1 5 10 15 Thr Arg Thr Leu 20 9520PRTHomo sapiens 95Glu Val Gln Ser Asp Tyr Ser Ser Tyr Ala Arg Thr Ser Ile Arg Ala 1 5 10 15 Ser Leu Thr Phe 20 9620PRTHomo sapiens 96Asn Arg Gly Phe Lys Ala Gly Arg Asn Met Arg Arg Lys Leu Phe Gly 1 5 10 15 Val Leu Arg Leu 20 9720PRTHomo sapiens 97Lys Cys His Ser Leu Phe Leu Asp Leu Gln Val Asn Ser Leu Gln Thr 1 5 10 15 Val Cys Thr Asn 20 9820PRTHomo sapiens 98Ile Tyr Lys Ile Leu Leu Leu Gln Ala Tyr Arg Phe His Ala Cys Val 1 5 10 15 Leu Gln Leu Pro 20 9920PRTHomo sapiens 99Phe His Gln Gln Val Trp Lys Asn Pro Thr Phe Phe Leu Arg Val Ile 1 5 10 15 Ser Asp Thr Ala 20 10020PRTHomo sapiens 100Ser Leu Cys Tyr Ser Ile Leu Lys Ala Lys Asn Ala Gly Met Ser Leu 1 5 10 15 Gly Ala Lys Gly 20 10120PRTHomo sapiens 101Ala Ala Gly Pro Leu Pro Ser Glu Ala Val Gln Trp Leu Cys His Gln 1 5 10 15 Ala Phe Leu Leu 20 10220PRTHomo sapiens 102Lys Leu Thr Arg His Arg Val Thr Tyr Val Pro Leu Leu Gly Ser Leu 1 5 10 15 Arg Thr Ala Gln 20 10320PRTHomo sapiens 103Thr Gln Leu Ser Arg Lys Leu Pro Gly Thr Thr Leu Thr Ala Leu Glu 1 5 10 15 Ala Ala Ala Asn 20 10412PRTHomo sapiens 104Pro Ala Leu Pro Ser Asp Phe Lys Thr Ile Leu Asp 1 5 10 10510PRTHomo sapiens 105Met Pro Arg Ala Pro Arg Cys Arg Ala Val 1 5 10 10620PRTHomo sapiens 106Arg Ser Leu Leu Arg Ser His Tyr Arg Glu Val Leu Pro Leu Ala Thr 1 5 10 15 Phe Val Arg Arg 20 10720PRTHomo sapiens 107Leu Gly Pro Gln Gly Trp Arg Leu Val Gln Arg Gly Asp Pro Ala Ala 1 5 10 15 Phe Arg Ala Leu 20 10820PRTHomo sapiens 108Val Ala Gln Cys Leu Val Cys Val Pro Trp Asp Ala Arg Pro Pro Pro 1 5 10 15 Ala Ala Pro Ser 20 10920PRTHomo sapiens 109Phe Arg Gln Val Ser Cys Leu Lys Glu Leu Val Ala Arg Val Leu Gln 1 5 10 15 Arg Leu Cys Glu 20 11020PRTHomo sapiens 110Arg Gly Ala Lys Asn Val Leu Ala Phe Gly Phe Ala Leu Leu Asp Gly 1 5 10 15 Ala Arg Gly Gly 20 11120PRTHomo sapiens 111Pro Pro Glu Ala Phe Thr Thr Ser Val Arg Ser Tyr Leu Pro Asn Thr 1 5 10 15 Val Thr Asp Ala 20 11220PRTHomo sapiens 112Leu Arg Gly Ser Gly Ala Trp Gly Leu Leu Leu Arg Arg Val Gly Asp 1 5 10 15 Asp Val Leu Val 20 11320PRTHomo sapiens 113His Leu Leu Ala Arg Cys Ala Leu Phe Val Leu Val Ala Pro Ser Cys 1 5 10 15 Ala Tyr Gln Val 20 11420PRTHomo sapiens 114Cys Gly Pro Pro Leu Tyr Gln Leu Gly Ala Ala Thr Gln Ala Arg Pro 1 5 10 15 Pro Pro His Ala 20 11520PRTHomo sapiens 115Ser Gly Pro Arg Arg Arg Leu Gly Cys Glu Arg Ala Trp Asn His Ser 1 5 10 15 Val Arg Glu Ala 20 11620PRTHomo sapiens 116Gly Val Pro Leu Gly Leu Pro Ala Pro Gly Ala Arg Arg Arg Gly Gly 1 5 10 15 Ser Ala Ser Arg 20 11720PRTHomo sapiens 117Ser Leu Pro Leu Pro Lys Arg Pro Arg Arg Gly Ala Ala Pro Glu Pro 1 5 10 15 Glu Arg Thr Pro 20 11820PRTHomo sapiens 118Val Gly Gln Gly Ser Trp Ala His Pro Gly Arg Thr Arg Gly Pro Ser 1 5 10 15 Asp Arg Gly Phe 20 11920PRTHomo sapiens 119Cys Val Val Ser Pro Ala Arg Pro Ala Glu Glu Ala Thr Ser Leu Glu 1

5 10 15 Gly Ala Leu Ser 20 12020PRTHomo sapiens 120Gly Thr Arg His Ser His Pro Ser Val Gly Arg Gln His His Ala Gly 1 5 10 15 Pro Pro Ser Thr 20 12120PRTHomo sapiens 121Ser Arg Pro Pro Arg Pro Trp Asp Thr Pro Cys Pro Pro Val Tyr Ala 1 5 10 15 Glu Thr Lys His 20 12220PRTHomo sapiens 122Phe Leu Tyr Ser Ser Gly Asp Lys Glu Gln Leu Arg Pro Ser Phe Leu 1 5 10 15 Leu Ser Ser Leu 20 12320PRTHomo sapiens 123Arg Pro Ser Leu Thr Gly Ala Arg Arg Leu Val Glu Thr Ile Phe Leu 1 5 10 15 Gly Ser Arg Pro 20 12420PRTHomo sapiens 124Trp Met Pro Gly Thr Pro Arg Arg Leu Pro Arg Leu Pro Gln Arg Tyr 1 5 10 15 Trp Gln Met Arg 20 12520PRTHomo sapiens 125Pro Leu Phe Leu Glu Leu Leu Gly Asn His Ala Gln Cys Pro Tyr Gly 1 5 10 15 Val Leu Leu Lys 20 12620PRTHomo sapiens 126Thr His Cys Pro Leu Arg Ala Ala Val Thr Pro Ala Ala Gly Val Cys 1 5 10 15 Ala Arg Glu Lys 20 12720PRTHomo sapiens 127Pro Gln Gly Ser Val Ala Ala Pro Glu Glu Glu Asp Thr Asp Pro Arg 1 5 10 15 Arg Leu Val Gln 20 12820PRTHomo sapiens 128Leu Leu Arg Gln His Ser Ser Pro Trp Gln Val Tyr Gly Phe Val Arg 1 5 10 15 Ala Cys Leu Arg 20 12920PRTHomo sapiens 129Arg Leu Val Pro Pro Gly Leu Trp Gly Ser Arg His Asn Glu Arg Arg 1 5 10 15 Phe Leu Arg Asn 20 13020PRTHomo sapiens 130Thr Lys Lys Phe Ile Ser Leu Gly Lys His Ala Lys Leu Ser Leu Gln 1 5 10 15 Glu Leu Thr Trp 20 13120PRTHomo sapiens 131Lys Met Ser Val Arg Asp Cys Ala Trp Leu Arg Arg Ser Pro Gly Val 1 5 10 15 Gly Cys Val Pro 20 13220PRTHomo sapiens 132Ala Ala Glu His Arg Leu Arg Glu Glu Ile Leu Ala Lys Phe Leu His 1 5 10 15 Trp Leu Met Ser 20 13320PRTHomo sapiens 133Val Tyr Val Val Glu Leu Leu Arg Ser Phe Phe Tyr Val Thr Glu Thr 1 5 10 15 Thr Phe Gln Lys 20 13420PRTHomo sapiens 134Asn Arg Leu Phe Phe Tyr Arg Lys Ser Val Trp Ser Lys Leu Gln Ser 1 5 10 15 Ile Gly Ile Arg 20 13520PRTHomo sapiens 135Gln His Leu Lys Arg Val Gln Leu Arg Glu Leu Ser Glu Ala Glu Val 1 5 10 15 Arg Gln His Arg 20 13620PRTHomo sapiens 136Glu Ala Arg Pro Ala Leu Leu Thr Ser Arg Leu Arg Phe Ile Pro Lys 1 5 10 15 Pro Asp Gly Leu 20 13720PRTHomo sapiens 137Arg Pro Ile Val Asn Met Asp Tyr Val Val Gly Ala Arg Thr Phe Arg 1 5 10 15 Arg Glu Lys Arg 20 13820PRTHomo sapiens 138Ala Glu Arg Leu Thr Ser Arg Val Lys Ala Leu Phe Ser Val Leu Asn 1 5 10 15 Tyr Glu Arg Ala 20 13920PRTHomo sapiens 139Arg Arg Pro Gly Leu Leu Gly Ala Ser Val Leu Gly Leu Asp Asp Ile 1 5 10 15 His Arg Ala Trp 20 14020PRTHomo sapiens 140Arg Thr Phe Val Leu Arg Val Arg Ala Gln Asp Pro Pro Pro Glu Leu 1 5 10 15 Tyr Phe Val Lys 20 14120PRTHomo sapiens 141Val Asp Val Thr Gly Ala Tyr Asp Thr Ile Pro Gln Asp Arg Leu Thr 1 5 10 15 Glu Val Ile Ala 20 14220PRTHomo sapiens 142Ser Ile Ile Lys Pro Gln Asn Thr Tyr Cys Val Arg Arg Tyr Ala Val 1 5 10 15 Val Gln Lys Ala 20 14320PRTHomo sapiens 143Ala His Gly His Val Arg Lys Ala Phe Lys Ser His Val Ser Thr Leu 1 5 10 15 Thr Asp Leu Gln 20 14420PRTHomo sapiens 144Pro Tyr Met Arg Gln Phe Val Ala His Leu Gln Glu Thr Ser Pro Leu 1 5 10 15 Arg Asp Ala Val 20 14520PRTHomo sapiens 145Val Ile Glu Gln Ser Ser Ser Leu Asn Glu Ala Ser Ser Gly Leu Phe 1 5 10 15 Asp Val Phe Leu 20 14620PRTHomo sapiens 146Arg Phe Met Cys His His Ala Val Arg Ile Arg Gly Lys Ser Tyr Val 1 5 10 15 Gln Cys Gln Gly 20 14720PRTHomo sapiens 147Ile Pro Gln Gly Ser Ile Leu Ser Thr Leu Leu Cys Ser Leu Cys Tyr 1 5 10 15 Gly Asp Met Glu 20 14820PRTHomo sapiens 148Asn Lys Leu Phe Ala Gly Ile Arg Arg Asp Gly Leu Leu Leu Arg Leu 1 5 10 15 Val Asp Asp Phe 20 14920PRTHomo sapiens 149Leu Leu Val Thr Pro His Leu Thr His Ala Lys Thr Phe Leu Arg Thr 1 5 10 15 Leu Val Arg Gly 20 15020PRTHomo sapiens 150Val Pro Glu Tyr Gly Cys Val Val Asn Leu Arg Lys Thr Val Val Asn 1 5 10 15 Phe Pro Val Glu 20 15120PRTHomo sapiens 151Asp Glu Ala Leu Gly Gly Thr Ala Phe Val Gln Met Pro Ala His Gly 1 5 10 15 Leu Phe Pro Trp 20 15220PRTHomo sapiens 152Cys Gly Leu Leu Leu Asp Thr Arg Thr Leu Glu Val Gln Ser Asp Tyr 1 5 10 15 Ser Ser Tyr Ala 20 15320PRTHomo sapiens 153Arg Thr Ser Ile Arg Ala Ser Leu Thr Phe Asn Arg Gly Phe Lys Ala 1 5 10 15 Gly Arg Asn Met 20 15420PRTHomo sapiens 154Arg Arg Lys Leu Phe Gly Val Leu Arg Leu Lys Cys His Ser Leu Phe 1 5 10 15 Leu Asp Leu Gln 20 15520PRTHomo sapiens 155Val Asn Ser Leu Gln Thr Val Cys Thr Asn Ile Tyr Lys Ile Leu Leu 1 5 10 15 Leu Gln Ala Tyr 20 15620PRTHomo sapiens 156Arg Phe His Ala Cys Val Leu Gln Leu Pro Phe His Gln Gln Val Trp 1 5 10 15 Lys Asn Pro Thr 20 15720PRTHomo sapiens 157Phe Phe Leu Arg Val Ile Ser Asp Thr Ala Ser Leu Cys Tyr Ser Ile 1 5 10 15 Leu Lys Ala Lys 20 15820PRTHomo sapiens 158Asn Ala Gly Met Ser Leu Gly Ala Lys Gly Ala Ala Gly Pro Leu Pro 1 5 10 15 Ser Glu Ala Val 20 15920PRTHomo sapiens 159Gln Trp Leu Cys His Gln Ala Phe Leu Leu Lys Leu Thr Arg His Arg 1 5 10 15 Val Thr Tyr Val 20 16020PRTHomo sapiens 160Pro Leu Leu Gly Ser Leu Arg Thr Ala Gln Thr Gln Leu Ser Arg Lys 1 5 10 15 Leu Pro Gly Thr 20 16122PRTHomo sapiens 161Thr Leu Thr Ala Leu Glu Ala Ala Ala Asn Pro Ala Leu Pro Ser Asp 1 5 10 15 Phe Lys Thr Ile Leu Asp 20 1621132PRTHomo sapiens 162Met Pro Arg Ala Pro Arg Cys Arg Ala Val Arg Ser Leu Leu Arg Ser 1 5 10 15 His Tyr Arg Glu Val Leu Pro Leu Ala Thr Phe Val Arg Arg Leu Gly 20 25 30 Pro Gln Gly Trp Arg Leu Val Gln Arg Gly Asp Pro Ala Ala Phe Arg 35 40 45 Ala Leu Val Ala Gln Cys Leu Val Cys Val Pro Trp Asp Ala Arg Pro 50 55 60 Pro Pro Ala Ala Pro Ser Phe Arg Gln Val Ser Cys Leu Lys Glu Leu 65 70 75 80 Val Ala Arg Val Leu Gln Arg Leu Cys Glu Arg Gly Ala Lys Asn Val 85 90 95 Leu Ala Phe Gly Phe Ala Leu Leu Asp Gly Ala Arg Gly Gly Pro Pro 100 105 110 Glu Ala Phe Thr Thr Ser Val Arg Ser Tyr Leu Pro Asn Thr Val Thr 115 120 125 Asp Ala Leu Arg Gly Ser Gly Ala Trp Gly Leu Leu Leu Arg Arg Val 130 135 140 Gly Asp Asp Val Leu Val His Leu Leu Ala Arg Cys Ala Leu Phe Val 145 150 155 160 Leu Val Ala Pro Ser Cys Ala Tyr Gln Val Cys Gly Pro Pro Leu Tyr 165 170 175 Gln Leu Gly Ala Ala Thr Gln Ala Arg Pro Pro Pro His Ala Ser Gly 180 185 190 Pro Arg Arg Arg Leu Gly Cys Glu Arg Ala Trp Asn His Ser Val Arg 195 200 205 Glu Ala Gly Val Pro Leu Gly Leu Pro Ala Pro Gly Ala Arg Arg Arg 210 215 220 Gly Gly Ser Ala Ser Arg Ser Leu Pro Leu Pro Lys Arg Pro Arg Arg 225 230 235 240 Gly Ala Ala Pro Glu Pro Glu Arg Thr Pro Val Gly Gln Gly Ser Trp 245 250 255 Ala His Pro Gly Arg Thr Arg Gly Pro Ser Asp Arg Gly Phe Cys Val 260 265 270 Val Ser Pro Ala Arg Pro Ala Glu Glu Ala Thr Ser Leu Glu Gly Ala 275 280 285 Leu Ser Gly Thr Arg His Ser His Pro Ser Val Gly Arg Gln His His 290 295 300 Ala Gly Pro Pro Ser Thr Ser Arg Pro Pro Arg Pro Trp Asp Thr Pro 305 310 315 320 Cys Pro Pro Val Tyr Ala Glu Thr Lys His Phe Leu Tyr Ser Ser Gly 325 330 335 Asp Lys Glu Gln Leu Arg Pro Ser Phe Leu Leu Ser Ser Leu Arg Pro 340 345 350 Ser Leu Thr Gly Ala Arg Arg Leu Val Glu Thr Ile Phe Leu Gly Ser 355 360 365 Arg Pro Trp Met Pro Gly Thr Pro Arg Arg Leu Pro Arg Leu Pro Gln 370 375 380 Arg Tyr Trp Gln Met Arg Pro Leu Phe Leu Glu Leu Leu Gly Asn His 385 390 395 400 Ala Gln Cys Pro Tyr Gly Val Leu Leu Lys Thr His Cys Pro Leu Arg 405 410 415 Ala Ala Val Thr Pro Ala Ala Gly Val Cys Ala Arg Glu Lys Pro Gln 420 425 430 Gly Ser Val Ala Ala Pro Glu Glu Glu Asp Thr Asp Pro Arg Arg Leu 435 440 445 Val Gln Leu Leu Arg Gln His Ser Ser Pro Trp Gln Val Tyr Gly Phe 450 455 460 Val Arg Ala Cys Leu Arg Arg Leu Val Pro Pro Gly Leu Trp Gly Ser 465 470 475 480 Arg His Asn Glu Arg Arg Phe Leu Arg Asn Thr Lys Lys Phe Ile Ser 485 490 495 Leu Gly Lys His Ala Lys Leu Ser Leu Gln Glu Leu Thr Trp Lys Met 500 505 510 Ser Val Arg Asp Cys Ala Trp Leu Arg Arg Ser Pro Gly Val Gly Cys 515 520 525 Val Pro Ala Ala Glu His Arg Leu Arg Glu Glu Ile Leu Ala Lys Phe 530 535 540 Leu His Trp Leu Met Ser Val Tyr Val Val Glu Leu Leu Arg Ser Phe 545 550 555 560 Phe Tyr Val Thr Glu Thr Thr Phe Gln Lys Asn Arg Leu Phe Phe Tyr 565 570 575 Arg Lys Ser Val Trp Ser Lys Leu Gln Ser Ile Gly Ile Arg Gln His 580 585 590 Leu Lys Arg Val Gln Leu Arg Glu Leu Ser Glu Ala Glu Val Arg Gln 595 600 605 His Arg Glu Ala Arg Pro Ala Leu Leu Thr Ser Arg Leu Arg Phe Ile 610 615 620 Pro Lys Pro Asp Gly Leu Arg Pro Ile Val Asn Met Asp Tyr Val Val 625 630 635 640 Gly Ala Arg Thr Phe Arg Arg Glu Lys Arg Ala Glu Arg Leu Thr Ser 645 650 655 Arg Val Lys Ala Leu Phe Ser Val Leu Asn Tyr Glu Arg Ala Arg Arg 660 665 670 Pro Gly Leu Leu Gly Ala Ser Val Leu Gly Leu Asp Asp Ile His Arg 675 680 685 Ala Trp Arg Thr Phe Val Leu Arg Val Arg Ala Gln Asp Pro Pro Pro 690 695 700 Glu Leu Tyr Phe Val Lys Val Asp Val Thr Gly Ala Tyr Asp Thr Ile 705 710 715 720 Pro Gln Asp Arg Leu Thr Glu Val Ile Ala Ser Ile Ile Lys Pro Gln 725 730 735 Asn Thr Tyr Cys Val Arg Arg Tyr Ala Val Val Gln Lys Ala Ala His 740 745 750 Gly His Val Arg Lys Ala Phe Lys Ser His Val Ser Thr Leu Thr Asp 755 760 765 Leu Gln Pro Tyr Met Arg Gln Phe Val Ala His Leu Gln Glu Thr Ser 770 775 780 Pro Leu Arg Asp Ala Val Val Ile Glu Gln Ser Ser Ser Leu Asn Glu 785 790 795 800 Ala Ser Ser Gly Leu Phe Asp Val Phe Leu Arg Phe Met Cys His His 805 810 815 Ala Val Arg Ile Arg Gly Lys Ser Tyr Val Gln Cys Gln Gly Ile Pro 820 825 830 Gln Gly Ser Ile Leu Ser Thr Leu Leu Cys Ser Leu Cys Tyr Gly Asp 835 840 845 Met Glu Asn Lys Leu Phe Ala Gly Ile Arg Arg Asp Gly Leu Leu Leu 850 855 860 Arg Leu Val Asp Asp Phe Leu Leu Val Thr Pro His Leu Thr His Ala 865 870 875 880 Lys Thr Phe Leu Arg Thr Leu Val Arg Gly Val Pro Glu Tyr Gly Cys 885 890 895 Val Val Asn Leu Arg Lys Thr Val Val Asn Phe Pro Val Glu Asp Glu 900 905 910 Ala Leu Gly Gly Thr Ala Phe Val Gln Met Pro Ala His Gly Leu Phe 915 920 925 Pro Trp Cys Gly Leu Leu Leu Asp Thr Arg Thr Leu Glu Val Gln Ser 930 935 940 Asp Tyr Ser Ser Tyr Ala Arg Thr Ser Ile Arg Ala Ser Leu Thr Phe 945 950 955 960 Asn Arg Gly Phe Lys Ala Gly Arg Asn Met Arg Arg Lys Leu Phe Gly 965 970 975 Val Leu Arg Leu Lys Cys His Ser Leu Phe Leu Asp Leu Gln Val Asn 980 985 990 Ser Leu Gln Thr Val Cys Thr Asn Ile Tyr Lys Ile Leu Leu Leu Gln 995 1000 1005 Ala Tyr Arg Phe His Ala Cys Val Leu Gln Leu Pro Phe His Gln 1010 1015 1020 Gln Val Trp Lys Asn Pro Thr Phe Phe Leu Arg Val Ile Ser Asp 1025 1030 1035 Thr Ala Ser Leu Cys Tyr Ser Ile Leu Lys Ala Lys Asn Ala Gly 1040 1045 1050 Met Ser Leu Gly Ala Lys Gly Ala Ala Gly Pro Leu Pro Ser Glu 1055 1060 1065 Ala Val Gln Trp Leu Cys His Gln Ala Phe Leu Leu Lys Leu Thr 1070 1075 1080 Arg His Arg Val Thr Tyr Val Pro Leu Leu Gly Ser Leu Arg Thr 1085 1090 1095 Ala Gln Thr Gln Leu Ser Arg Lys Leu Pro Gly Thr Thr Leu Thr 1100 1105 1110 Ala Leu Glu Ala Ala Ala Asn Pro Ala Leu Pro Ser Asp Phe Lys 1115 1120 1125 Thr Ile Leu Asp 1130 16316PRTHomo sapiens 163Glu Ala Arg Pro Ala Leu Leu Thr Ser Arg Leu Arg Phe Ile Pro Lys 1 5 10 15

Claims

1. A peptide with anti-inflammatory activity, wherein the peptide comprises (a) at least one amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 161, or (b) at least one amino acid sequence having at least 80% sequence identity with an amino acid sequence defined in (a), or (c) a fragment of an amino acid sequence defined in (a) or (b).

2. The peptide according to claim 1, wherein the fragment consists of at least 3 amino acids.

3. The peptide according to claim 1, wherein the peptide consists of at most 30.

4. The peptide according to claim 1, which consists of any one amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 161.

5. The peptide according to claim 1, which comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1 to SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 14 to 21, SEQ ID NO: 23 to SEQ ID NO: 37, SEQ ID NO: 39 to SEQ ID NO: 44, SEQ ID NO: 47 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 61, SEQ ID NO: 63 to SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 99 to SEQ ID NO: 104, SEQ ID NO: 107 to SEQ ID NO: 109, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 120 to SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 129 to SEQ ID NO: 133, SEQ ID NO: 142 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, and SEQ ID NO: 155 to SEQ ID NO: 159.

6. The peptide according to claim 5, which comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 15 to SEQ ID NO: 18, SEQ ID NO: 23 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31 to SEQ ID NO: 34, SEQ ID NO: 39 to SEQ ID NO: 41, SEQ ID NO: 47, SEQ ID NO:48, SEQ ID NO: 51 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 58, SEQ ID NO: 61, SEQ ID NO: 65 to SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 73 to SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 87, SEQ ID NO: 8=90 to SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 99, SEQ ID NO: 101 to SEQ ID NO: 104, SEQ ID NO: 107 to SEQ ID NO: 109, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 129 to SEQ ID NO: 132, SEQ ID NO: 142 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, and SEQ ID NO: 157 to SEQ ID NO: 159.

7. The peptide according to claim 1, which comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1 to SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 32 to SEQ ID NO: 53, SEQ ID NO: 55 to SEQ ID NO: 60, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72 to SEQ ID NO: 82, SEQ ID NO: 84 to SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 99 to SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 127 to SEQ ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 151 and SEQ ID NO: 153 to SEQ ID NO: 161.

8. The peptide according to claim 7, which comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1 to SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17 to SEQ ID NO: 23, SEQ ID NO: 25 to SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 33 to SEQ ID NO: 43, SEQ ID NO: 156, SEQ ID NO: 157 and SEQ ID NO: 159.

9. The peptide according to claim 1, which comprises any one amino acid sequence selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 52, SEQ ID NO: 57, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 91, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 118, SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 129, SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 135, SEQ ID NO: 146, SEQ ID NO: 151, SEQ ID NO: 154, and SEQ ID NO: 156.

10. The peptide according to claim 1, which originates from human telomerase.

11. A polynucleotide encoding a peptide according to any one of the preceding claims.

12. An anti-inflammatory composition comprising a peptide according to any one of claims 1-10 as active ingredient.

13. The anti-inflammatory composition according to claim 12 for use in a method for treatment or prophylaxis of inflammatory disease.

14. The anti-inflammatory composition according to claim 12, which is a cosmetic composition for improving or preventing skin inflammation.

15. The anti-inflammatory composition according to claim 12, which is a pharmaceutical composition for treatment or prophylaxis of inflammatory diseases.

16. The anti-inflammatory composition according to claim 12, which is a food composition for treatment or prophylaxis of inflammation.

17. The anti-inflammatory composition according to claim 13 or 15, wherein the inflammatory disease is selected from the group consisting of (1) general or localized inflammatory disease (for example, allergies; immune-complex disease; hayfever; hypersensitive shock; endotoxin shock; cachexia, hyperthermia; granulomatosis; or sarcoidosis); (2) gastro-intestinal related diseases (for example, appendicitis; gastric ulcer; duodenal ulcer; peritonitis; pancreatitis; ulcerative, acute, or ischemic colitis; cholangitis; cholecystitis, steatorrhea, hepatitis, Crone's disease; or Whipple's Disease); (3) dermal related diseases (for example, psoriasis; burns; sunburns; dermatitis; Urticarial warts or wheal); (4) vascular related diseases (for example, angiitis; vasculitis; endocarditis; arteritis; atherosclerosis; thrombophlebitis; pericarditis; congestive heart failure; myocarditis; myocardial ischemia; periarteritis nodosa; recurrent stenosis; Buerger's disease; or rheumatic fever); (5) respiratory diseases (for example, asthma; epiglottitis; bronchitis; emphysema; rhinitis; cystic fibrosis; interstitial pneumonitis; COPD (chronic obstructive pulmonary disease); adult respiratory distress syndrome; coniosis; alveolitis; bronchiolitis; pharyngitis; pleurisy; or sinusitis); (6) bone, joint, muscle and connective tissue related diseases (for example, eosinophilic granuloma; arthritis; arthralgia; osteomyelitis; dermatomyositis; fasciitis; Paget's disease; gout; periodontal disease; rheumatoid arthritis; myasthenia gravis; ankylosing spondylitis; or synovitis); (7) urogenital disorders (for example, epididymitis; vaginitis; prostatitis; or urethritis); (8) central or peripheral nervous system related diseases (for example, Alzheimer's disease; meningitis; encephalitis; multiple sclerosis; cerebral infarction; cerebral embolism; Guillain-Barre syndrome; neuritis; neuralgia; spinal cord injury; paralysis; or uveitis); (9) virus (for example, influenza; respiratory syncytial virus; HIV; hepatitis B; hepatitis C; or herpes virus), infectious disease (for example, Dengue fever; or septicemia), fungal infection (for example, candidiasis); or bacterial, parasitic, and similar microbial infection (for example, disseminated bacteremia; malaria; onchocerciasis; or amebiasis); (10) autoimmune disease (for example, thyroiditis; lupus; Goodpasture's syndrome; allograft rejection; graft versus host disease; or diabetes); and (11) cancer or tumor disease (for example, Hodgkin's disease).

18. A method for treating or preventing inflammatory diseases by administering the anti-inflammatory composition according to any one of claims 12 to 17.

19. A kit for prophylaxis or treatment of inflammatory diseases comprising: a peptide according to any one of claims 1-10; and instructions including at least one of administration dose, administration route, administration frequency, and indication of the peptide or composition.

20. The peptide according to any one of claims 1-10 for use as a pharmaceutical.

21. The peptide according to any one of claims 1-10 for use in a method according to claim 18.