U.S. patent application number 14/250136 was filed with the patent office on 2015-04-16 for cosmetic composition containing fermented ginseng berry pleurotus ferulae product.
This patent application is currently assigned to AMI COSMETIC CO., LTD.. The applicant listed for this patent is AMI COSMETIC CO., LTD.. Invention is credited to Il Hong, Do Gyeong Lee, Jung No Lee, Kyung Rok Lee, Nu Rim Lee, Sung Min Park.
Application Number | 20150104529 14/250136 |
Document ID | / |
Family ID | 49989275 |
Filed Date | 2015-04-16 |
United States Patent
Application |
20150104529 |
Kind Code |
A1 |
Lee; Kyung Rok ; et
al. |
April 16, 2015 |
COSMETIC COMPOSITION CONTAINING FERMENTED GINSENG BERRY PLEUROTUS
FERULAE PRODUCT
Abstract
Disclosed are a fermented ginseng berry Pleurotus ferulae
product and a cosmetic composition containing the same. The
fermented ginseng berry Pleurotus ferulae product is useful for
anti-oxidation, anti-inflammation, collagen synthesis facilitation,
skin wrinkle care, whitening, moisturizing, skin barrier
improvement and atopy alleviation.
Inventors: |
Lee; Kyung Rok; (Seoul,
KR) ; Hong; Il; (Seoul, KR) ; Lee; Do
Gyeong; (Gyeongsan, KR) ; Park; Sung Min;
(Cheongwon-Gun, KR) ; Lee; Jung No; (Cheongju,
KR) ; Lee; Nu Rim; (Jeollanam-do, KR) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
AMI COSMETIC CO., LTD. |
Seoul |
|
KR |
|
|
Assignee: |
AMI COSMETIC CO., LTD.
Seoul
KR
|
Family ID: |
49989275 |
Appl. No.: |
14/250136 |
Filed: |
April 10, 2014 |
Current U.S.
Class: |
424/728 |
Current CPC
Class: |
A61K 2800/85 20130101;
A61Q 19/08 20130101; A61P 29/00 20180101; A61Q 19/00 20130101; A61Q
19/02 20130101; A61K 8/9789 20170801; A61P 37/08 20180101; C12P
1/02 20130101; A61K 2800/522 20130101; A61K 8/9728 20170801 |
Class at
Publication: |
424/728 |
International
Class: |
A61K 8/97 20060101
A61K008/97; A61Q 19/02 20060101 A61Q019/02; A61Q 19/00 20060101
A61Q019/00; A61Q 19/08 20060101 A61Q019/08 |
Foreign Application Data
Date |
Code |
Application Number |
Oct 11, 2013 |
KR |
10-2013-0120984 |
Claims
1. A cosmetic composition comprising, as an active ingredient, a
fermented ginseng berry Pleurotus ferulae product obtained by
fermenting a ginseng berry extract with Pleurotus ferulae.
2. The cosmetic composition according to claim 1, wherein the
cosmetic composition is used for anti-oxidation.
3. The cosmetic composition according to claim 1, wherein the
cosmetic composition is used for anti-inflammation.
4. The cosmetic composition according to claim 1, wherein the
cosmetic composition is used for skin wrinkle care.
5. The cosmetic composition according to claim 1, wherein the
cosmetic composition is used for skin whitening.
6. The cosmetic composition according to claim 1, wherein the
cosmetic composition is used for skin moisturizing.
7. The cosmetic composition according to claim 1, wherein the
cosmetic composition is used for atopy alleviation.
8. The cosmetic composition according to claim 1, wherein the
ginseng berry extract is extracted using any one extraction solvent
selected from the group consisting of water, C1-C4 anhydrous or
aqueous lower alcohol, acetone, ethyl acetate, butyl acetate and
1,3-butylene glycol.
9. The cosmetic composition according to claim 1, wherein the
fermented ginseng berry Pleurotus ferulae product is present in an
amount of 0.0001 to 100.0% by weight, with respect to the total
weight of the cosmetic composition.
10. A cosmetic composition comprising, as an active ingredient, an
extract of a fermented ginseng berry Pleurotus ferulae product,
obtained by fermenting a ginseng berry extract with Pleurotus
ferulae.
11. The cosmetic composition according to claim 10, wherein the
cosmetic composition is used for anti-oxidation.
12. The cosmetic composition according to claim 10, wherein the
cosmetic composition is used for anti-inflammation.
13. The cosmetic composition according to claim 10, wherein the
cosmetic composition is used for skin wrinkle care.
14. The cosmetic composition according to claim 10, wherein the
cosmetic composition is used for skin whitening.
15. The cosmetic composition according to claim 10, wherein the
cosmetic composition is used for skin moisturizing.
16. The cosmetic composition according to claim 10, wherein the
cosmetic composition is used for atopy alleviation.
17. The cosmetic composition according to claim 10, wherein the
ginseng berry extract is extracted using any one extraction solvent
selected from the group consisting of water, C1-C4 anhydrous or
aqueous lower alcohol, acetone, ethyl acetate, butyl acetate and
1,3-butylene glycol.
18. The cosmetic composition according to claim 10, wherein the
fermented ginseng berry Pleurotus ferulae product is present in an
amount of 0.0001 to 100.0% by weight, with respect to the total
weight of the cosmetic composition.
Description
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims priority to Korean Application No.
10-2013-0120984 filed on Oct. 11, 2013, which is incorporated
herein by reference.
TECHNICAL BACKGROUND
[0002] The present invention relates to a cosmetic composition
containing, as an active ingredient, a fermented ginseng berry
Pleurotus ferulae product. More specifically, the present invention
relates to a cosmetic composition containing, as an active
ingredient, a fermented product prepared by fermenting a ginseng
berry extract with Pleurotus ferulae mycelium.
RELATED ART
[0003] Aging of the skin is classified into two types, i.e.,
intrinsic (chronological) aging and photoaging [Gilchrest B A: J.
Am. Acad. Dermatol., 21, 610-613 (1989)]. Intrinsic aging naturally
occurs due to decrease in physiologic functions with age [Braverman
I M, et al.: J. Invest. Dermatol., 78, 434-443 (1982)]. Photoaging
means change associated with appearance and functions of the skin,
caused by repeated exposure of the skin to solar radiation [Ridder
G M et al.: J. Am. Acad. Dermatol., 25, 751-760 (1991)]. In
addition, aging of the skin may be caused by ultraviolet radiation,
stress, disease conditions, environmental factors, wounds and
activation of active oxygen species with age. As such conditions
worsen, antioxidant defense mechanisms present in vivo are
destructed, cells and tissues are damaged, and adult diseases and
aging are facilitated. More specifically, oxidation of lipids,
proteins, polysaccharides, nucleic acids and the like, which are
primary components of the skin, and destruction of skin cells and
tissues thus causes aging of the skin. In particular, oxidation of
proteins involves cleavage of collagen, hyaluronic acid, elastin,
proteoglycan, fibronetin and the like, which are connective tissues
of the skin, results in over-inflammation, causes damage to
elasticity of the skin and, in serious cases, brings about
mutations caused by DNA modification, emergence of cancers and
deterioration in immune function.
[0004] Accordingly, it is necessary to protect the skin through
scavenging of active oxygen species produced during in vivo
metabolic processes or active oxygen species mediated by
ultraviolet irradiation and inflammation, and to degenerate and
proliferate damaged cells through active metabolism so as to
restore the skin and keep the skin healthy. An enzyme called
"matrix metalloproteinase (MMP)" as well as active oxygen species
is mediated in aging. Synthesis and decomposition of the
extracellular matrix such as collagen are suitably controlled in
vivo, but the synthesis is deteriorated with aging, expression of
collagenase, i.e., matrix metalloproteinase (MMP) is facilitated,
elasticity of the skin is deteriorated and wrinkles are created. In
addition, exposure to ultraviolet radiation may cause activation of
such a decomposition enzyme. Thus, there is a demand for
development of substances which control expression of MMP activated
in cells by ultraviolet radiation or inhibit the activity of MMP.
Most ingredients used as raw materials of cosmetics to date simply
inhibit only activity of matrix metalloproteinases (MMPs).
[0005] Meanwhile, melanin is produced through conversion of
tyrosine into dopa, dopaquinone and then dopachrome by actions of
tyrosinase present in pigment cells. Melanin is present in the
skin, which protects the body from ultraviolet radiation and has an
essential function on control of hormone secretion in vivo.
However, over-production of melanin is known to create spots,
freckles and the like, accelerate aging of the skin and play an
important role in inducing skin cancers. As such, research and
development to prevent melanin over-production is actively
underway. Ascorbic acid (Japanese Patent Publication Sho. 4-9320),
hydroquinone (Japanese Patent Publication Sho. 6-192062), kojic
acid (Japanese Patent Publication Hei. 56-7710), arbutin (Japanese
Patent Publication Sho. 4-93150), plant extracts and the like have
already been used for whitening cosmetics owing to inhibitory
activity against tyrosinase, but use thereof is limited due to
problems such as decomposition and discoloration caused by bad
stability in cosmetic formulations, generation of off-flavor,
unclear in vivo efficacies and effects, and safety.
[0006] Meanwhile, human skin is an organ which serves as a
protective membrane which protects human from external
environments, and functions to prevent loss of biogenic substances
such as water and electrolytes and prevent harmful substances from
invading into the human. Skin is broadly divided into the
epidermis, the dermis and subcutaneous fat. The epidermis is
composed of keratinocytes and melanocytes. A keratinocyte layer
which constitutes the outermost layer of the skin directly contacts
external environments and thus should have high physical or
chemical resistance or superior barrier property to materials,
prevent release (loss) of water to the outside from the human, and
maintain flexibility due to presence of a proper amount of water
therein.
[0007] In general, content of water in the skin is about 70% for
the dermis, but content of water gradually decreases from the
dermis to the epidermis and the keratinocyte layer. The content of
water in the keratinocyte layer is about 10% to about 30%. The
water supplied from the dermis is predominantly moved to an upper
part of the keratinocyte layer via passive diffusion and is finally
discharged to the outside. This phenomenon is referred to as a
"trans-epidermal water loss (TEWL)" and lipid membranes, i.e.,
sebum and cuticular lipids, of the keratinocyte layer, are known to
regulate such TEWL to a proper level.
[0008] Meanwhile, a hydrophilic substance capable of retaining
water, called "natural moisturizing factor (NMF)", present in the
keratinocyte layer is known to play an important role in
moisturizing the skin. When a normal keratinocyte layer maintains a
water content of about 10 to about 30%, the skin becomes smooth and
soft and normally exerts the function of protecting the body.
However, when the water content of the keratinocyte layer is 10% or
less, the skin becomes rough, losses its body protection function
and is aged. For example, in the case of the dry skin, a scaling
phenomenon in which scale-like keratinocytes are peeled off the
surface of the skin due to weak adhesion between keratinocytes
occurs. The dryness of the skin is due to the fact that the dry
skin has a water content of the keratinocyte layer lower than that
of normal skin. In addition, even healthy skin has bad conditions
due to lack of water caused by exposure to harsh external
environments, i.e., wind, cold weather, sunlight, washing or
shaving.
[0009] Accordingly, it is considerably important to suitably
maintain a water content of the skin keratinocyte layer. For this
purpose, cosmetics containing components similar to sebum, NMF
components or moisturizers such as polyols were used. For example,
glycerin or sorbitol having three or more hydroxyl groups (OH
groups), as water-soluble polyols, exhibit excellent moisturizing
effect, but renders discomfort upon use due to severe stickiness,
and propylene glycol, 1,3-butylene glycol and the like having two
hydroxyl groups (OH groups) cause side effects to the skin. In
addition, other natural moisturizing factors such as pyrrolidone
carboxylate sodium (PCA-Na), sodium lactate and urea have a problem
of impairing emulsion stability due to high electrolytic property.
Amino acids, collagen, elastin and the like have moisturizing
properties, but the moisturizing properties thereof are
limited.
[0010] The water retention property of the keratinocyte layer may
be controlled by natural moisturizing factors (NMFs) composed of
amino acids, lactic acid, urea and inorganic salts. Development of
substances for external application to the skin, which are safe,
and have excellent usability and superior moisturizing effects like
natural moisturizing factors, is an important research issue in
cosmetics.
[0011] On the other hand, in recent years, cosmetics using natural
substances are used to reduce skin irritation caused by chemicals.
A development value of natural substances as raw materials for
cosmetics is gradually increased because the natural substances
reduce side effects in the skin and the response of recent cosmetic
consumers to cosmetics using natural ingredients is increased.
[0012] As a result of research into applicability of a variety of
natural substances to cosmetics, the inventors of the present
invention discovered that an extract of ginseng berries prepared by
fermentation with Pleurotus ferulae mycelium exhibits
anti-oxidation, anti-inflammation, collagen synthesis promotion,
skin wrinkle care, whitening, moisturizing, skin barrier function
and atopy alleviation effects. The present invention was completed
based on this discovery.
[0013] (Patent Document) Korean Patent No. 10-0887631 (registered
on Mar. 2, 2009) discloses a composition for external application
to the skin containing a ginseng berry extract and a cosmetic
containing the ginseng berry extract as an active ingredient.
SUMMARY OF THE INVENTION
[0014] Therefore, the present invention has been made in view of
the above problems, and it is an object of the present invention to
develop and provide a cosmetic composition using a ginseng berry
which is applicable to skin cosmetics and has considerably safety
due to non-harmness to the human body.
[0015] In accordance with the present invention, the above and
other objects can be accomplished by the provision of a cosmetic
composition containing, as an active ingredient, a fermented
ginseng berry Pleurotus ferulae product obtained by fermenting a
ginseng berry extract with Pleurotus ferulae.
[0016] Meanwhile, the cosmetic composition of the present invention
is for example used for any one selected from anti-oxidation,
anti-inflammation, skin wrinkle care, skin whitening, skin
moisturizing and atopy alleviation.
[0017] Meanwhile, in the cosmetic composition according to the
present invention, the ginseng berry extract is for example
extracted using any one extraction solvent selected from the group
consisting of water, C1-C4 anhydrous or aqueous lower alcohol,
acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol.
[0018] Meanwhile, regarding the cosmetic composition according to
the present invention, the fermented ginseng berry Pleurotus
ferulae product is preferably present in an amount of 0.0001 to
100.0% by weight, with respect to the total weight of the cosmetic
composition.
BRIEF DESCRIPTION OF THE DRAWINGS
[0019] The above and other objects, features and other advantages
of the present invention will be more clearly understood from the
following detailed description taken in conjunction with the
accompanying drawings, in which:
[0020] FIG. 1 is a graph showing comparison in free radical
scavenging activity at different concentrations (0.001%, 0.002%,
0.005%, 0.01%) between a ginseng berry extract obtained in
Preparation Example 1 and fermented ginseng berry products obtained
in Example 1 and Comparative Examples 1 to 4, wherein `EGCG`
represents epigallocatechin gallate, `a` represents a ginseng berry
extract of Preparation Example 1, `b` represents a fermented
ginseng berry Pleurotus ferulae product of Example 1, `c`
represents a fermented ginseng berry Tricholoma matsutake product
of Comparative Example 1, `d` represents a fermented ginseng berry
Sarcodon aspratus product of Comparative Example 2, `e` represents
a fermented ginseng berry Phellinus linteus product of Comparative
Example 3, and `f` represents a fermented ginseng berry Ganoderma
lucidum product of Comparative Example 4;
[0021] FIG. 2 is a graph showing comparison in 5-lipoxygenase
inhibition activity at different concentrations (0.001%, 0.002%,
0.005%, 0.01%) between the ginseng berry extract obtained in
Preparation Example 1 and the fermented ginseng berry products
obtained in Example 1 and Comparative Examples 1 to 4, wherein
`NDGA` represents nordihydroguaiaretic acid, `a` represents a
ginseng berry extract of Preparation Example 1, `b` represents a
fermented ginseng berry Pleurotus ferulae product of Example 1, `c`
represents a fermented ginseng berry Tricholoma matsutake product
of Comparative Example 1, `d` represents a fermented ginseng berry
Sarcodon aspratus product of Comparative Example 2, `e` represents
a fermented ginseng berry Phellinus linteus product of Comparative
Example 3, and `f` represents a fermented ginseng berry Ganoderma
lucidum product of Comparative Example 4;
[0022] FIG. 3 is a graph showing comparison in collagen synthesis
at different concentrations (0.001%, 0.002%, 0.005%, 0.01%) between
the ginseng berry extract obtained in Preparation Example 1 and the
fermented ginseng berry products obtained in Example 1 and
Comparative Examples 1 to 4, wherein `L-AA` represents L-ascorbic
acid, `a` represents a ginseng berry extract of Preparation Example
1, `b` represents a fermented ginseng berry Pleurotus ferulae
product of Example 1, `c` represents a fermented ginseng berry
Tricholoma matsutake product of Comparative Example 1, `d`
represents a fermented ginseng berry Sarcodon aspratus product of
Comparative Example 2, `e` represents a fermented ginseng berry
Phellinus linteus product of Comparative Example 3, and `f`
represents a fermented ginseng berry Ganoderma lucidum product of
Comparative Example 4;
[0023] FIG. 4 is a graph showing comparison in MMP-1 inhibition
activity at different concentrations (0.001%, 0.002%, 0.005%,
0.01%) between the ginseng berry extract obtained in Preparation
Example 1 and the fermented ginseng berry products obtained in
Example 1 and Comparative Examples 1 to 4, wherein `retinol`
represents retinol, `a` represents a ginseng berry extract of
Preparation Example 1, `b` represents a fermented ginseng berry
Pleurotus ferulae product of Example 1, `c` represents a fermented
ginseng berry Tricholoma matsutake product of Comparative Example
1, `d` represents a fermented ginseng berry Sarcodon aspratus
product of Comparative Example 2, `e` represents a fermented
ginseng berry Phellinus linteus product of Comparative Example 3,
and `f` represents a fermented ginseng berry Ganoderma lucidum
product of Comparative Example 4; and
[0024] FIG. 5 is a graph showing comparison in inhibition of
tyrosinase activity at different concentrations (0.001%, 0.002%,
0.005%, 0.01%) between the ginseng berry extract obtained in
Preparation Example 1 and the fermented ginseng berry products
obtained in Example 1 and Comparative Examples 1 to 4, wherein
`Kojic acid` represents kojic acid, `a` represents a ginseng berry
extract of Preparation Example 1, `b` represents a fermented
ginseng berry Pleurotus ferulae product of Example 1, `c`
represents a fermented ginseng berry Tricholoma matsutake product
of Comparative Example 1, `d` represents a fermented ginseng berry
Sarcodon aspratus product of Comparative Example 2, `e` represents
a fermented ginseng berry Phellinus linteus product of Comparative
Example 3, and `f` represents a fermented ginseng berry Ganoderma
lucidum product of Comparative Example 4.
DETAILED DESCRIPTION OF THE INVENTION
[0025] Hereinafter, embodiments of the prevent invention will be
described in detail.
[0026] Meanwhile, Panax ginseng C. A. Meyter used for the present
invention which is called "ginseng berry" is the fruit of the 4
years or more old ginseng plant harvested for only about one week
in the middle of July. Ginseng berry extracts have been widely
utilized in a variety of applications to date. There is no research
demonstrating the fact discovered by the present invention that the
extract of ginseng berries fermented with Pleurotus ferulae
exhibits considerably superior anti-oxidation, anti-inflammation,
collagen synthesis facilitation, skin wrinkle care, whitening,
moisturizing, skin barrier improvement and atopy alleviation
effects, as compared to ginseng berry extracts.
[0027] Meanwhile, Pleurotus ferulae Lenzi is a kind of mushroom
which belongs to pleurototaceae and pleurotus. Pleurotus ferulae is
the name given to call to grow in glassroots called "ferula
asafetida" in Xinjiang, China and has been artificially cultivated
since 1983. Pleurotus ferulae is known to have been first found in
the Madonic Mountain, the Sicily Island, Italy in 1963. Pleurotus
ferulae is known to be a nutrient repository. Pleurotus ferulae
having a delicate and soft pine fragrance had been used in China
from old times due to potent medical efficacies including treatment
of stomach and kidney disorders, cough drop, removal of
inflammation and prevention of diseases of obstetrics and
gynecology.
[0028] The present inventors fermented ginseng berries with a
variety of mushrooms as raw materials. As a result, the present
inventors discovered that the ginseng berry fermented with
Pleurotus ferulae mycelium exhibited superior anti-oxidation,
anti-inflammation, collagen synthesis facilitation, skin wrinkle
care, whitening, moisturizing, skin barrier improvement and atopy
alleviation effects. The present invention was completed based on
this discovery.
[0029] Meanwhile, regarding the cosmetic composition of the present
invention, the ginseng berry extract may be prepared using a common
solvent in accordance with an ordinary method well-known in the
art, that is, under ordinary temperature and pressure conditions.
For example, the ginseng berry extract which is the active
ingredient of the composition of the present invention may be
prepared by extracting ginseng berries using a solvent selected
from the group consisting of water, C1-C4 anhydrous or aqueous
lower alcohol, acetone, ethyl acetate, butyl acetate and
1,3-butylene glycol, and optionally mixing the extract. The solvent
is preferably alcohol, more preferably ethanol, even more
preferably, 70% ethanol. The extraction solvent is preferably added
in an amount of 1 to 15-fold, more preferably 5 to 10-fold, most
preferably 7-fold, of a dry weight of the ginseng berry.
[0030] Meanwhile, the fermented ginseng berry Pleurotus ferulae
product according to the present invention may include an extract
obtained by the extraction method described above and an extract
obtained by a subsequent common purification process. For example,
the fermented ginseng berry Pleurotus ferulae product includes
fragments obtained by further performing a variety of purification
processes such as separation using ultrafiltration membranes having
a constant cut-off value and separation using a variety of
chromatography (manufactured for separation according to size,
charges, and hydrophobicity or hydrophilicity).
[0031] In addition, the fermented ginseng berry Pleurotus ferulae
product according to the present invention may include a fermented
product prepared in the form of a powder, obtained by a further
process such as distillation under reduced pressure, and
lyophilization or spray-drying.
[0032] Meanwhile, in a preferred embodiment of the present
invention, the content of the fermented ginseng berry Pleurotus
ferulae product in the cosmetic composition of the present
invention is preferably 0.0001 to 100.0% by weight, more preferably
0.001 to 90.0% by weight, most preferably 0.1 to 85.0% by weight,
with respect to the total weight of the cosmetic composition.
[0033] Meanwhile, as can be seen from the experiment described
below, the fermented ginseng berry Pleurotus ferulae product
according to the present invention superior anti-oxidation effect
(see Experimental Example 1), superior anti-inflammatory effect
(see Experimental Example 3), superior collagen synthesis effect
(see Experimental Example 4), superior wrinkle alleviation effect
(see Experimental Example 5), and superior whitening effect (see
Experimental Example 6).
[0034] It is demonstrated that the cosmetic composition prepared
using the fermented ginseng berry Pleurotus ferulae product having
the effects described above, as an active ingredient, exhibits
superior skin moisturizing improvement effect (see Experimental
Example 7), superior skin barrier improvement effect (see
Experimental Example 8), and superior atopy alleviation effect (see
Experimental Example 9).
[0035] Meanwhile, the cosmetic composition according to the present
invention may have any one formulation selected from the group
consisting of solutions, suspensions, emulsions, pastes, gels,
creams, lotions, powders, soaps, surfactant-containing cleansings,
oils, powder foundations, emulsion foundations, wax foundations and
sprays, but the present invention is not necessarily limited
thereto.
[0036] Meanwhile, the composition of the present invention may
further include additives commonly used in cosmetics, such as
hydrophilic or lyphophilic gelling agents, hydrophilic or
lyphophilic activators, preservatives, antioxidants, solvents,
flavoring agents, fillers, blockers, pigments, deodorant agents and
dyes. These additives may be present in amounts commonly used in
the art and may be for example 0.0001 to 100.0% by weight with
respect to the total weight of the cosmetic composition. The
additives and contents thereof are selected such that preferred
features of the cosmetic composition according to the present
invention are not impaired.
EXAMPLE
[0037] The present invention will be described in more detail with
reference to the following Examples. The scope of the present
invention is not limited to the following examples and covers
modifications of the technical spirit substantially equivalent
thereto.
Preparation Example 1
Preparation of Ginseng Berry Extract
[0038] Ginseng berries were washed with distilled water, dried,
thoroughly crushed and passed through a 100 mesh sieve. The crushed
ginseng berry granules were added to 70% ethanol such that a
concentration of the resulting mixture was adjusted to 150 g/L,
extracted under reflux for 5 hours three times and then macerated.
Then, the resulting product was filtered through Whatman #3 filter
paper, concentrated under reduced pressure at 50.degree. C. or less
and lyophilized.
Example 1
Preparation of Fermented Ginseng Berry Pleurotus ferulae
Product
[0039] 1 to 4% of glucose was added to 5 g of the ginseng berry
concentrate prepared in Preparation Example 1, and 0.2 to 0.5% of
peptone was further added thereto, followed by sterilization. The
sterilized product was inoculated at a concentration of 25 g/L with
a Pleurotus ferulae Lenzi. strain culture liquid, followed by
culturing. The culturing was performed in a 5 L fermenter for 7
days at 37.degree. C. and a pH of 5 to 7. After culturing, the
culture solution was centrifuged to primarily remove culture strain
and sterilized (121.degree. C., 15 minutes, 1.5 atm) to prevent
further culturing.
[0040] Ethanol was added to the fermented ginseng berry product
obtained by fermentation and then primary removal of culture
bacteria so as to obtain a final 70% (V/V) aqueous ethanol
solution, extracted under reflux for 5 hours three times, macerated
and filtered through Whatman #3 filter paper. After filtering, the
resulting extract was concentrated in a concentrator under reduced
pressure at 50.degree. C. or less and lyophilized.
Comparative Example 1
Preparation of Fermented Ginseng Berry Tricholoma matsutake
Product
[0041] In Comparative Example 1, the ginseng berry extract obtained
in Preparation Example 1 was fermented with Tricholoma matsutake
mycelium to prepare a fermented ginseng berry Tricholoma matsutake
product. The present experiment was performed in the same manner as
in Example 1 except that Tricholoma matsutake was used, instead of
Pleurotus ferulae.
Comparative Example 2
Preparation of Fermented Ginseng Berry Sarcodon aspratus
Product
[0042] In Comparative Example 1, the ginseng berry extract obtained
in Preparation Example 1 was fermented with Sarcodon aspratus
mycelium to prepare a fermented ginseng berry Sarcodon aspratus
product. The present experiment was performed in the same manner as
in Example 1 except that Sarcodon aspratus was used, instead of
Pleurotus ferulae.
Comparative Example 3
Preparation of Fermented Ginseng Berry Phellinus linteus
Product
[0043] In Comparative Example 1, the ginseng berry extract obtained
in Preparation Example 1 was fermented with Phellinus linteus
mycelium to prepare a fermented ginseng berry Phellinus linteus
product. The present experiment was performed in the same manner as
in Example 1 except that Phellinus linteus was used, instead of
Pleurotus ferulae.
Comparative Example 4
Preparation of Fermented Ginseng Berry Ganoderma lucidum
Product
[0044] In Comparative Example 1, the ginseng berry extract obtained
in Preparation Example 1 was fermented with Ganoderma lucidum
mycelium to prepare a fermented ginseng berry Ganoderma lucidum
product. The present experiment was performed in the same manner as
in Example 1 except that Ganoderma lucidum was used, instead of
Pleurotus ferulae.
Experimental Example 1
Evaluation of Antioxidative Activity of Ginseng Berry Extract and
Fermented Ginseng Berry Product Using DPPH Method
[0045] A powder of the ginseng berry extract obtained in
Preparation Example 1 and powders of fermented ginseng berry
products obtained in Example 1 and Comparative Examples 1 to 4 were
suspended in distilled water and free radical scavenging activities
thereof were evaluated at different concentrations (0.001%, 0.002%,
0.005%, 0.01%).
[0046] The DPPH (2,2-diphenyl-1-picrylhydrazyl) method is used to
measure variation in color from absorbance at 540 nm when
scavenging DPPH (2,2-Diphenyl-1-picrylhydrazyl) which is a radical
whose inhibitors are stable). Samples used for this experiment are
shown in the following Table 1 and free radical scavenging activity
was measured using the following Equation 1. Epigallocatechin
gallate (EGCG) was used as a control group. Epigallocatechin
gallate (EGCG, epigallocatechin-3-gallate) is a polyphenol
extracted from green tea leaves, which has been found to be a
potent antioxidant.
TABLE-US-00001 TABLE 1 Blank of Blank of Experimental experimental
Control control group group group Materials group (Blank of C)
(Exp. Group) (Blank of E) A DPPH 180 .mu.l (180 .mu.l 180 .mu.l
(180 .mu.l (MeOH) MeOH) MeOH) B Sample (20 .mu.l (20 .mu.l solvent)
20 .mu.l 20 .mu.l solvent)
Scavenging activity ( % ) = [ ( C - D ) - ( A - B ) ] ( C - D )
.times. 100 Equation 1 ##EQU00001##
[0047] A: Abs of experimental group
[0048] B: Abs of blank of experimental group
[0049] C: Abs of control group
[0050] D: Abs of blank of control group
[0051] Meanwhile, among fermented mushroom products, the fermented
Pleurotus ferulae product (Example 1) exhibited the highest free
radical scavenging activity. As free radical scavenging activity
increases, antioxidative activity increases. Accordingly, the
fermented ginseng berry Pleurotus ferulae product exhibits
antioxidative activity higher than the ginseng berry extract.
[0052] Meanwhile, as can be seen from the results of FIG. 1, the
fermented ginseng berry products (Example 1 and Comparative
Examples 1 to 4) exhibited superior free radical scavenging
activity at all different concentrations as compared to the ginseng
berry extract (Preparation Example 1). In FIG. 1, `a` represents a
ginseng berry extract of Preparation Example 1, `b` represents a
fermented ginseng berry Pleurotus ferulae product of Example 1, `c`
represents a fermented ginseng berry Tricholoma matsutake product
of Comparative Example 1, `d` represents a fermented ginseng berry
Sarcodon aspratus product of Comparative Example 2, `e` represents
a fermented ginseng berry Phellinus linteus product of Comparative
Example 3, and `f` represents a fermented ginseng berry Ganoderma
lucidum product of Comparative Example 4.
Experimental Example 2
Evaluation of Effects of Ginseng Berry Extract and Fermented
Ginseng Berry Product on Cell Survival Rate
[0053] Effects of the ginseng berry extract obtained in Preparation
Example 1 and the fermented ginseng berry products obtained in
Example 1 and Comparative Examples 1 to 4 on cells were measured.
The ginseng berry extract powder obtained in Preparation Example 1
and fermented ginseng berry product powders obtained in Example 1
and Comparative Examples 1 to 4 were suspended in distilled water
to obtain different concentrations (0.001%, 0.002%, 0.005%, 0.01%)
of suspensions and cell survival rates of the suspensions were
measured in accordance with the following method.
[0054] Cytotoxicty was measured by a Mosmann method which measures
cell survival rate using a MTT
{3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium
bromide}reagent.
[0055] HDF was seeded at a concentration of 1.times.10.sup.4
cells/well on a 96-well plate and cultured under conditions of
37.degree. C. and 5% CO.sub.2 for 24 hours. After a culture medium
was removed, the HDF was cultured in a medium treated with the
sample at different concentrations for 24 hours, and the medium was
removed and washed twice with phosphate buffered saline (PBS). MTT
was dissolved at a concentration of 5 mg/mL in PBS, 50 L of the
resulting MTT solution was added to HDF and HDF was cultured under
the conditions of 37.degree. C. and 5% CO.sub.2 for 2 hours. 100 L
of dimethyl sulfoxide (DMSO) was added to each well, followed by
stirring for 10 minutes. Then, absorbance at 40 nm was
measured.
[0056] As a result of measurement, respective samples did not
exhibit cytotoxicty at all concentrations (not shown). This result
demonstrates that the fermented ginseng berry Pleurotus ferulae
product according to the present invention is harmless to human
body and has considerably excellent stability.
Experimental Example 3
Evaluation of Anti-Inflammatory Effect (5-Lipoxygenase Inhibitory
Activity) of Ginseng Berry Extract and Fermented Ginseng Berry
Product
[0057] The ginseng berry extract obtained in Preparation Example 1
and the fermented ginseng berry products obtained in Example 1 and
Comparative Examples 1 to 4 were suspended in distilled water and
anti-inflammatory effects thereof were evaluated by performing a
5-lipoxygenase inhibitory activity test under different
concentration conditions (0.001%, 0.002%, 0.005%, 0.01%).
[0058] Lipoxygenase is an enzyme which produces various chemical
mediators involved in inflammatory or allergic reaction in the
body. Inhibitors of lipoxygenase cause inhibition of production of
a variety of chemical mediators and are thus effective in
alleviating allergies. That is, as 5-lipoxygenase inhibitory
capacity increases, anti-inflammatory effect increases. In the
present experimental example, activity of lipoxygenase may be
indicated by peroxide production measured using substrates and
enzymes.
[0059] The present experiment was performed using samples shown in
the following Table 2 and 5-lipoxygenase inhibitory activity was
measured using Equation 1. The control group was
nordihydroguaiaretic acid (NDGA) which is a potent antioxidant of
fats and oils.
TABLE-US-00002 TABLE 2 Blank of Blank of Experimental experimental
Control control group group group Materials group (Blank of C)
(Exp. Group) (Blank of E) A Buffer 2 mL 2 mL 2 mL 2 mL B Sample 20
.mu.l 20 .mu.l 20 .mu.l 20 .mu.l solvent solvent C Enzyme 40 .mu.l
-- 20 .mu.l -- D Substrate 70 .mu.l 70 .mu.l 70 .mu.l 70 .mu.l
[0060] As can be seen from results shown in FIG. 2, the fermented
ginseng berry products of the present invention (Example 1 and
Comparative Examples 1 to 4) exhibited superior 5-lipoxygenase
inhibitory capacity to the ginseng berry extract (Preparation
Example 1). In particular, among the fermented products, the
fermented Pleurotus ferulae product (Example 1) exhibited the
highest 5-lipoxygenase inhibitory capacity and the fermented
ginseng berry Pleurotus ferulae product exhibited higher
anti-inflammatory effects than the ginseng berry extract.
[0061] Meanwhile, in FIG. 2, `a` represents a ginseng berry extract
of Preparation Example 1, `b` represents a fermented ginseng berry
Pleurotus ferulae product of Example 1, `c` represents a fermented
ginseng berry Tricholoma matsutake product of Comparative Example
1, `d` represents a fermented ginseng berry Sarcodon aspratus
product of Comparative Example 2, `e` represents a fermented
ginseng berry Phellinus linteus product of Comparative Example 3,
and `f` represents a fermented ginseng berry Ganoderma lucidum
product of Comparative Example 4.
Experimental Example 4
Evaluation of Collagen Synthesis of Ginseng Berry Extract and
Fermented Ginseng Berry Product
[0062] The ginseng berry extract obtained in Preparation Example 1
and the fermented ginseng berry product powders obtained in Example
1 and Comparative Examples 1 to 4 were suspended in distilled water
and a collagen biosynthesis increase at different concentrations
(0.001%, 0.002%, 0.005%, 0.01%) was evaluated.
[0063] Human dermal fibroblasts (HDFs) were seeded on a 24-well
plate at a concentration of 5.times.10.sup.4 cells/well and
cultured under the conditions of 37.degree. C. and 5% CO.sub.2 for
24 hours, and were further cultured in serum-free DMEMs (Dulbecco's
Modified Eagle's Mediums) containing the ginseng berry extract
obtained in Preparation Example 1 and the fermented ginseng berry
products obtained in Example 1 and Comparative Examples 1 to 4 and
in a serum-free DMEM medium not containing the fermented ginseng
berry product and the ginseng berry extract, as a control group.
After culturing, the supernatant present on each well was
collected, and an amount of procollagen type I C-peptide (PICP) was
measured using a collagen kit and was calculated in terms of ng/ml.
As a result, an amount of synthesized collagen was measured.
Collagen biosynthesis increase (%) was calculated in accordance
with the following Equation 2 and L-ascorbic acid (L-AA) having the
effect of facilitating collagen synthesis was used as a control
group.
Collagen biosynthesis increase (%)=collagen amount of experimental
group/collagen amount of control group.times.100 Equation 2
[0064] As can be seen from results shown in FIG. 3, the fermented
ginseng berry products of the present invention (Example 1 and
Comparative Examples 1 to 4) exhibited higher collagen synthesis at
all the concentrations than the ginseng berry extract (Preparation
Example 1). In particular, among the fermented products, the
fermented Pleurotus ferulae product (Example 1) exhibited the
highest collagen synthesis.
[0065] Meanwhile, in FIG. 3, `a` represents a ginseng berry extract
of Preparation Example 1, `b` represents a fermented ginseng berry
Pleurotus ferulae product of Example 1, `c` represents a fermented
ginseng berry Tricholoma matsutake product of Comparative Example
1, `d` represents a fermented ginseng berry Sarcodon aspratus
product of Comparative Example 2, `e` represents a fermented
ginseng berry Phellinus linteus product of Comparative Example 3,
and `f` represents a fermented ginseng berry Ganoderma lucidum
product of Comparative Example 4.
Experimental Example 5
Evaluation of Effects of Ginseng Berry Extracts and Fermented
Ginseng Berry Products on Wrinkle Alleviation
[0066] The ginseng berry extract obtained in Preparation Example 1
and the fermented ginseng berry product powders obtained in Example
1 and Comparative Examples 1 to 4 were suspended in distilled
water, and wrinkle alleviation effects at different concentrations
(0.001%, 0.002%, 0.005%, 0.01%) were evaluated.
[0067] For this experiment, human dermal fibroblasts were
irradiated with UVA at an energy of 5 J/cm.sup.2 in a UV chamber.
Conditions of the dose of ultraviolet radiation and culture time
which maximize expression of matrix metalloproteinase (MMP-1) in
fibroblasts were established through preliminary testing. A
negative control group was wrapped with a foil and exposed to UVA
for the same time. Here, UVA dose was measured using a UV
radiometer. The cells during irradiation of UVA were cultured in
the medium previously used. After UVA irradiation, the cells were
cultured in a fresh medium containing samples for 24 hours and
96-well plates were coated with the medium. The cells were treated
with primary antibodies {MMP-1 (Ab-5) monoclonal antibody, MMP-1
(Ab-3) monoclonal antibody} and reaction was conducted at
37.degree. C. for 60 minutes. The cells were reacted with
anti-mouse IgG (whole mouse, alkaline phosphatase conjugated) as a
secondary antibody for about 60 minutes, and were then reacted with
an alkaline phosphatase substrate solution (1 mg/mL
.rho.-nitrophenyl phosphate in diethanolamine buffer solution) at
room temperature for 30 minutes, and absorbance at 405 nm was
measured using a microplate reader. A control group was not treated
with the sample.
[0068] As can be seen from results shown in FIG. 4, at all
concentrations (0.001%, 0.002%, 0.005%, 0.01%), the fermented
ginseng berry products of the present invention (Example 1 and
Comparative Examples 1 to 4) exhibited superior MMP-1 expression
inhibitory capacity to the ginseng berry extract (Preparation
Example 1).
[0069] In particular, among the fermented products, the fermented
Pleurotus ferulae product (Example 1) exhibited the highest MMP-1
exhibition inhibitory capacity. Generally, as MMP-1 exhibition
inhibitory capacity increases, skin wrinkle alleviation effect
increases. Accordingly, the fermented ginseng berry Pleurotus
ferulae product exhibited superior skin wrinkle alleviation effect
to the ginseng berry extract.
[0070] Meanwhile, in FIG. 4, `a` represents a ginseng berry extract
of Preparation Example 1, `b` represents a fermented ginseng berry
Pleurotus ferulae product of Example 1, `c` represents a fermented
ginseng berry Tricholoma matsutake product of Comparative Example
1, `d` represents a fermented ginseng berry Sarcodon aspratus
product of Comparative Example 2, `e` represents a fermented
ginseng berry Phellinus linteus product of Comparative Example 3,
and `f` represents a fermented ginseng berry Ganoderma lucidum
product of Comparative Example 4.
Experimental Example 6
Effect of Fermented Ginseng Berry Pleurotus ferulae Product on
Inhibition of Tyrosinase
[0071] The following experiment was performed to confirm the effect
of a cosmetic containing the fermented ginseng berry Pleurotus
ferulae product according to the present invention on tyrosinase
inhibition. Tyrosinase (EC 1. 14. 18. 1) as an enzyme was dissolved
in a 0.05M phosphate buffer (pH 6.5) such that a concentration of
1,100 unit/ml (final 55 unit) was obtained, L-tyrosine
(C.sub.9H.sub.11NO.sub.3, 181.19) as a substrate was dissolved in a
0.1M phosphate buffer (pH 6.5) such that a concentration of 1.5 mM
(final 225 uM) was obtained, the resulting solutions were each
diluted with 0.1M sodium phosphate buffer (pH 6.5), and reacted
(Table 3) and further reacted at 37.degree. C. for 20 minutes, and
absorbance at 492 nm was measured using an ELISA reader
(enzyme-linked immunossorbent assay). Kojic acid, which has effects
of inhibiting tyrosinase activity and inhibiting production of
melanine, was used as a control group.
TABLE-US-00003 TABLE 3 Blank of Blank of Experimental experimental
Control control group group group Materials group (Blank of C)
(Exp. Group) (Blank of E) A Buffer 140 .mu.l 150 .mu.l 140 .mu.l
150 .mu.l B Sample (20 .mu.l (20 .mu.l 20 .mu.l 20 .mu.l solvent)
solvent) C Enzyme 10 .mu.l -- 10 .mu.l -- D Substrate 30 .mu.l 30
.mu.l 30 .mu.l 30 .mu.l
Inhibition ( % ) = [ ( C - D ) - ( A - B ) ] ( C - D ) .times. 100
Equation 3 ##EQU00002##
[0072] A: Abs of experimental group
[0073] B: Abs of blank of experimental group
[0074] C: Abs of control group
[0075] D: Abs of blank of control group
[0076] As can be seen from results shown in FIG. 5, at all
concentrations (0.001%, 0.002%, 0.005%, 0.01%), the fermented
ginseng berry products of the present invention (Example 1 and
Comparative Examples 1 to 4) exhibited superior tyrosinase
inhibitory capacity to the ginseng berry extract (Preparation
Example 1).
[0077] In particular, among the fermented products, the fermented
ginseng berry Pleurotus ferulae product exhibited superior
tyrosinase inhibition effect (whitening effect) to the ginseng
berry extract.
[0078] Tyrosinase (EC 1.14.18.1) is an important enzyme which
performs multiple enzymatic functions in the melanosynthetic
pathway. In general, as tyrosinase inhibitory capacity increases,
effects associated with skin whitening and propagation inhibition
of melanoma formed by malignant alteration of melanine cells
increase.
[0079] Meanwhile, in FIG. 5, `a` represents a ginseng berry extract
of Preparation Example 1, `b` represents a fermented ginseng berry
Pleurotus ferulae product of Example 1, `c` represents a fermented
ginseng berry Tricholoma matsutake product of Comparative Example
1, `d` represents a fermented ginseng berry Sarcodon aspratus
product of Comparative Example 2, `e` represents a fermented
ginseng berry Phellinus linteus product of Comparative Example 3,
and `f` represents a fermented ginseng berry Ganoderma lucidum
product of Comparative Example 4.
Formulation Example 1 and Formulation Comparative Example 1
Preparation of Cosmetic Containing Fermented Ginseng Berry
Pleurotus ferulae Product
[0080] A cosmetic composition containing 30.0% by weight of the
fermented ginseng berry Pleurotus ferulae product obtained in
Example 1 was prepared under the composition conditions shown in
the following Table 4 and was referred to as "Formulation Example
1", and a cosmetic composition not containing the fermented ginseng
berry Pleurotus ferulae product was prepared and referred to as
"Formulation Comparative Example 1".
TABLE-US-00004 TABLE 4 Content of formulation Content of
Formulation Example 1 Comparative Example 1 Ingredients (wt %) (wt
%) Preparation -- 30.0 Example 1 Example 1 30.0 -- 1,3-BG 10.0 10.0
Glycerine 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl acetate 3.0
3.0 Liquid paraffin 4.6 4.6 Triethylolamine 1.0 1.0 Squalane 3.1
3.1 Macadamia nut oil 2.5 2.5 Polysorbate 60 1.6 1.6 Sorbitan
sesquoleate 1.6 1.6 Propyl paraben 0.6 0.6 Carboxyvinyl polymer 1.5
1.5 Flavor Trace Trace Preservative Trace Trace Distilled water
Balance Balance Total 100 100
Experimental Example 7
Effect of Fermented Ginseng Berry Pleurotus ferulae Product on
Improvement of Skin Moisturizing
[0081] The following experiment was performed in order to confirm
the effect of the cosmetic containing the fermented ginseng berry
Pleurotus ferulae product according to the present invention on
improvement of skin moisturizing power.
[0082] 40 subjects in their 20s to 40s who have no skin diseases
were divided into two groups, each group having 20 subjects, a
nutrient cream of Formulation Example 1 and a nutrient cream of
Formulation Comparative Example 1 were applied over the entire
region of the face twice per day for one month. Prior to
application, skin conductance was measured using a corneometer
(CM820 courage Khazaka electronic GmbH, Germany) under constant
temperature and humidity conditions (24.degree. C., humidity 40%)
and was set to a reference value, skin conductance increase (%) was
measured after one, two and four weeks, and increases thereof were
evaluated. The results are shown in the following Table 5.
TABLE-US-00005 TABLE 5 Items After one week After two weeks After
four weeks Formulation 55 59 64 Example 1 Formulation 26 30 36
Comparative Example 1 (Unit: %)
[0083] As a result of the experiment, Formulation Example 1, the
cosmetic containing the fermented ginseng berry Pleurotus ferulae
product according to the present invention exhibited a considerably
high skin conductance increase, as compared to Formulation
Comparative Example 1. In general, skin conductance increases in
proportion to skin water content, the cosmetic containing the
fermented ginseng berry Pleurotus ferulae product according to the
present invention maintained high skin water content, as compared
to a cosmetic not containing the fermented ginseng berry Pleurotus
ferulae product (not shown).
Experimental Example 8
Effect of Fermented Ginseng Berry Pleurotus ferulae Product on
Improvement of Skin Barrier Function
[0084] The following experiment was performed in order to confirm
the effect of the cosmetic containing the fermented ginseng berry
Pleurotus ferulae product according to the present invention on
improvement of skin barrier.
[0085] In the present experimental example, transepidemal water
losses (TEWL) of humans to whom the cosmetic of Formulation Example
1 and the cosmetic of Formulation Comparative Example 1 were
applied were measured using a Tewameter (TM300, Courage and Khazaka
Electronic Co., Germany) and were then compared. The samples were
applied to the right and left sides of faces of 40 women in their
20s to 40s, transepidemal water loss of an area spaced 3 cm apart
to the right and 1 cm apart downward from the eye site was measured
three times using Tewameter (TM300, Courage and Khazaka Electronic
Co., Germany) before application and 1 hour, 2 hours, 4 hours and 6
hours after application and an average of three transepidemal water
loss values was calculated.
TABLE-US-00006 TABLE 6 Items 1 hour 2 hours 4 hours 6 hours
Formulation 15.5 14.6 12.2 9.2 Example 1 Formulation 18.1 16.9 13.8
11.2 Comparative Example 1 (Unit: g/h/m.sup.2)
[0086] As a result of measurement of transepidemal water loss, as
can be seen from Table 6, transepidemal water loss on the facial
skin of subjects to whom the cream prepared in Formulation Example
1 was applied was decreased. This result demonstrated that the
cosmetic containing the fermented ginseng berry Pleurotus ferulae
product according to the present invention exhibited superior skin
barrier improvement effect, as compared to cosmetics not containing
the fermented ginseng berry Pleurotus ferulae product (not
shown).
Experimental Example 9
Effect of Fermented Ginseng Berry Pleurotus ferulae Product on
Atopy Alleviation
[0087] The following experiment was performed in order to confirm
the effect of the cosmetic containing the fermented ginseng berry
Pleurotus ferulae product according to the present invention on
atopy alleviation. Among 20 subjects (3-40 olds) who suffered from
atopy, the cream of Formulation Example 1 and the cream of
Formulation Comparative Example 1 were continuously applied to the
atopy skin site of 10 subjects and the remaining 10 subjects,
respectively, twice per day over four weeks. Atopy dermatitis
severity (0 point: none, 10 point: weak, 20 point: mild, 30 point:
sever, 40 point: extremely sever), IgE variation, eosinophil
variation, water content and skin acidity on the test site of the
subjects were measured before application of the product (0 week)
and at four weeks after application of the product. All the
subjects who participated in the experiment had no abnormal
response.
[0088] The following Table 7 shows comparison in atopy alleviation
between subjects treated with creams prepared in Formulation
Example 1 and Formulation Comparative Example 1.
TABLE-US-00007 TABLE 7 Items Atopy derma- IgE Eosinophil Water Skin
titis severity variation variation content acidity (point) (mg/dl)
(ea) (g/cm.sup.2h) (pH) Before After Before After Before After
Before After Before After Formulation 37.41 28.12 435.9 321.4 317
249 25.84 26.57 5.719 6.071 Example 1 Formulation 37.68 31.53 443.7
363.8 321 275 26.31 25.11 5.717 5.833 Comparative Example 1 n = 20,
p < 0.05
[0089] As a result of the experiment, subjects to whom the cream
containing the fermented ginseng berry Pleurotus ferulae product of
Formulation Example 1 was applied were excellent in terms of atopy
dermatitis severity as atopy skin diagnostic criteria, variation in
IgE and eosinophil in blood, water content and improvement in skin
acidity. The cosmetic containing the fermented ginseng berry
Pleurotus ferulae product according to the present invention
exhibited superior atopy alleviation to cosmetics not containing
the fermented ginseng berry Pleurotus ferulae product (not
shown).
[0090] As apparent from the fore-going, the fermented ginseng berry
Pleurotus ferulae product according to the present invention
exhibits considerably superior anti-oxidation, anti-inflammation,
collagen synthesis facilitation, skin wrinkle care, whitening,
moisturizing, skin barrier improvement and atopy alleviation
effects, as compared to a ginseng berry extract.
[0091] Accordingly, the present invention also provides a cosmetic
composition having superior functionality.
[0092] Although the preferred embodiments of the present invention
have been disclosed for illustrative purposes, those skilled in the
art will appreciate that various modifications, additions and
substitutions are possible, without departing from the scope and
spirit of the invention as disclosed in the accompanying
claims.
* * * * *