U.S. patent application number 14/506512 was filed with the patent office on 2015-04-09 for fc variants with altered binding to fcrn.
The applicant listed for this patent is Xencor, Inc.. Invention is credited to Aaron Keith Chamberlain, Bassil Dahiyat, John R. Desjarlais, Sher Bahadur Karki, Gregory Alan Lazar.
Application Number | 20150099863 14/506512 |
Document ID | / |
Family ID | 46332096 |
Filed Date | 2015-04-09 |
United States Patent
Application |
20150099863 |
Kind Code |
A1 |
Chamberlain; Aaron Keith ;
et al. |
April 9, 2015 |
Fc VARIANTS WITH ALTERED BINDING TO FcRn
Abstract
The present application relates to a variant Fc region
comprising at least one modification relative to a wild-type human
Fc region, where the modification selected from the group
consisting of 434S, 252Y/428L, 252Y/434S, and 428L/434S, and the
numbering is according to the EU index.
Inventors: |
Chamberlain; Aaron Keith;
(Pasadena, CA) ; Dahiyat; Bassil; (Altadena,
CA) ; Desjarlais; John R.; (Pasadena, CA) ;
Karki; Sher Bahadur; (Santa Monica, CA) ; Lazar;
Gregory Alan; (Indianapolis, CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Xencor, Inc. |
Monrovia |
CA |
US |
|
|
Family ID: |
46332096 |
Appl. No.: |
14/506512 |
Filed: |
October 3, 2014 |
Related U.S. Patent Documents
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Application
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Patent Number |
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12697939 |
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8883973 |
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14506512 |
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12341769 |
Dec 22, 2008 |
8367805 |
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12697939 |
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11932151 |
Oct 31, 2007 |
8802820 |
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12341769 |
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11436266 |
May 17, 2006 |
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11932151 |
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11274065 |
Nov 14, 2005 |
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11436266 |
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60726453 |
Oct 12, 2005 |
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60703018 |
Jul 27, 2005 |
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60696609 |
Jul 5, 2005 |
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60690200 |
Jun 13, 2005 |
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60681607 |
May 16, 2005 |
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60669311 |
Apr 6, 2005 |
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60662468 |
Mar 15, 2005 |
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60649508 |
Feb 2, 2005 |
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60642886 |
Jan 11, 2005 |
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60627763 |
Nov 12, 2004 |
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61099178 |
Sep 22, 2008 |
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61079779 |
Jul 10, 2008 |
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61050172 |
May 2, 2008 |
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61046353 |
Apr 18, 2008 |
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61031353 |
Feb 25, 2008 |
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61016793 |
Dec 26, 2007 |
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60951536 |
Jul 24, 2007 |
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Current U.S.
Class: |
530/387.3 |
Current CPC
Class: |
C07K 16/2893 20130101;
A61P 29/00 20180101; A61K 2039/505 20130101; A61P 25/00 20180101;
Y02A 50/30 20180101; C07K 16/22 20130101; C07K 16/241 20130101;
C07K 16/2863 20130101; C07K 2319/30 20130101; Y02A 50/484 20180101;
A61P 37/00 20180101; C07K 2317/24 20130101; C07K 14/7151 20130101;
C07K 16/2896 20130101; C07K 2317/52 20130101; C07K 2317/90
20130101; C07K 16/18 20130101; A61P 31/00 20180101; C07K 16/32
20130101; C07K 2317/526 20130101; C07K 16/082 20130101; C07K
16/2866 20130101; C07K 16/4291 20130101; C07K 2317/524
20130101 |
Class at
Publication: |
530/387.3 |
International
Class: |
C07K 16/28 20060101
C07K016/28; C07K 16/24 20060101 C07K016/24; C07K 16/22 20060101
C07K016/22 |
Claims
1. A variant Fc region comprising at least one modification
relative to a wild-type human Fc region, wherein said modification
selected from the group consisting of: 434S, 252Y/428L, 252Y/434S,
and 428L/434S, wherein numbering is according to the EU index.
2. A variant according to claim 1, wherein said modification is
434S.
3. A variant according to claim 1, wherein said modification is
428L/434S.
4. A variant according to claim 1, wherein said modification is
252Y/428L.
5. A variant according to claim 1, wherein said modification is
252Y/434S.
6. A variant according to claim 1, wherein said variant comprises
an antibody or immunoadhesin, and wherein said Fc region is
selected from the group consisting of: IgG1, IgG2, IgG3, and
IgG4.
7. A variant according to claim 6, wherein said variant comprises
an IgG1 Fc region.
8. A variant according to claim 6, wherein said variant comprises
an IgG2 Fc region.
9. A variant according to claim 6, wherein said variant has longer
serum half-life in a mammal relative to said antibody or
immunoadhesin with said wild-type Fc region.
10. A variant according to claim 9, wherein said mammal is a mouse
expressing human FcRn or a non-human primate.
11. A variant according to claim 9, wherein said mammal is
human.
12. A variant according to claim 6, wherein said antibody or
immunoadhesin has specificity for an antigen selected from the
group consisting of: vascular endothelial growth factor (VEGF),
tumor necrosis factor .alpha. (TNF-.alpha.), CD25, epidermal growth
factor receptor (EGFR) and IgE.
13. A variant antibody or variant immunoadhesin comprising a
variant of an IgG2 Fc region, wherein said variant antibody or
immunoglobulin comprises between one and five modifications
relative to the wild-type IgG2 Fc region, wherein at least one of
said modifications is at position 434, wherein numbering is
according to the EU index, and wherein said variant antibody or
variant immunoadhesin has longer serum half-life in a mammal
relative to said antibody or immunoadhesin with said wild-type IgG2
Fc region.
14. A variant antibody or variant immunoadhesin according to claim
13, wherein said modification is 434S.
15. A variant according to claim 14, wherein said variant
additionally comprises at least one modification at a position
selected from the group consisting of: 252 and 428.
16. A variant according to claim 13, wherein said mammal is a mouse
expressing human FcRn or a non-human primate.
17. A variant according to claim 13, wherein said mammal is
human.
18. A variant Fc region comprising at least two modifications
relative to a wild-type human Fc region, wherein at least one
modification is 428L, and at least one modification is at position
252 or 434, wherein numbering is according to the EU index,
19. A variant according to claim 18, wherein said Fc region
comprises an antibody or immunoadhesin, and wherein said antibody
or immunoadhesin comprises an IgG1 or IgG2 Fc region, and wherein
said variant has longer serum half-life in a mammal relative to
said antibody or immunoadhesin with said wild-type human Fc region,
wherein said mammal is a mouse expressing human FcRn, a non-human
primate, or in humans.
Description
[0001] This application is a continuation of U.S. application Ser.
No. 12/697,939, filed Feb. 1, 2010, which is a divisional of U.S.
application Ser. No. 12/341,769, filed Dec. 22, 2008 which claims
benefit under 35 U.S.C. .sctn.119(e) to U.S. Ser. No. 61/016,793,
filed Dec. 26, 2007; U.S. Ser. No. 61/031,353, filed Feb. 25, 2008;
U.S. Ser. No. 61/046,353, filed Apr. 18, 2008; U.S. Ser No.
61/050,172, filed May 2, 2008; U.S. Ser. No. 61/079,779, filed Jul.
10, 2008; and U.S. Ser. No. 61/099,178, filed Sep. 22, 2008; and is
a continuation-in-part of U.S. Ser. No. 11/932,151, filed Oct. 31,
2007, which is a continuation-in-part of U.S. Ser. No. 11/436,266,
filed May 17, 2006, which claimed benefit under 35 U.S.C.
.sctn.119(e) to U.S. Ser. No. 60/951,536 filed Jul. 24, 2007, and
is a continuation-in-part of U.S. Ser. No. 11/274,065, filed on
Nov. 14, 2005, which claimed benefit under 35 U.S.C. .sctn.119(e)
to U.S. Ser. No. 60/627,763, filed Nov. 12, 2004; U.S. Ser. No.
60/642,886, filed Jan. 11, 2005; U.S. Ser. No. 60/649,508, filed
Feb. 2, 2005; U.S. Ser. No. 60/662,468, filed Mar. 15, 2005; U.S.
Ser. No. 60/669,311, filed Apr. 6, 2005; U.S. Ser. No. 60/681,607,
filed May 16, 2005; U.S. Ser. No. 60/690,200, filed Jun. 13, 2005;
U.S. Ser. No. 60/696,609, filed Jul. 5, 2005; U.S. Ser. No.
60/703,018, filed Jul. 27, 2005; and U.S. Ser. No. 60/726,453,
filed Oct. 12, 2005, all entirely incorporated by reference.
FIELD OF THE INVENTION
[0002] The present application relates to optimized IgG
immunoglobulin variants, engineering methods for their generation,
and their application, particularly for therapeutic purposes.
BACKGROUND OF THE INVENTION
[0003] Antibodies are immunological proteins that each binds a
specific antigen. In most mammals, including humans and mice,
antibodies are constructed from paired heavy and light polypeptide
chains. Each chain is made up of individual immunoglobulin (Ig)
domains, and thus the generic term immunoglobulin is used for such
proteins. Each chain is made up of two distinct regions, referred
to as the variable and constant regions. The light and heavy chain
variable regions show significant sequence diversity between
antibodies, and are responsible for binding the target antigen. The
constant regions show less sequence diversity, and are responsible
for binding a number of natural proteins to elicit important
biochemical events. In humans there are five different classes of
antibodies including IgA (which includes subclasses IgA1 and IgA2),
IgD, IgE, IgG (which includes subclasses IgG1, IgG2, IgG3, and
IgG4), and IgM. The distinguishing feature between these antibody
classes is their constant regions, although subtler differences may
exist in the V region. IgG antibodies are tetrameric proteins
composed of two heavy chains and two light chains. The IgG heavy
chain is composed of four immunoglobulin domains linked from N- to
C-terminus in the order VH--CH1--CH2--CH3, referring to the heavy
chain variable domain, heavy chain constant domain 1, heavy chain
constant domain 2, and heavy chain constant domain 3 respectively
(also referred to as VH--C.gamma.1--C.gamma.2--C.gamma.3, referring
to the heavy chain variable domain, constant gamma 1 domain,
constant gamma 2 domain, and constant gamma 3 domain respectively).
The IgG light chain is composed of two immunoglobulin domains
linked from N- to C-terminus in the order VL-CL, referring to the
light chain variable domain and the light chain constant domain
respectively.
[0004] In IgG, a site on Fc between the C.gamma.2 and C.gamma.3
domains mediates interaction with the neonatal receptor FcRn.
Binding to FcRn recycles endocytosed antibody from the endosome
back to the bloodstream (Raghavan et al., 1996, Annu Rev Cell Dev
Biol 12:181-220; Ghetie et al., 2000, Annu Rev Immunol 18:739-766,
both entirely incorporated by reference). This process, coupled
with preclusion of kidney filtration due to the large size of the
full-length molecule, results in favorable antibody serum
half-lives ranging from one to three weeks. Binding of Fc to FcRn
also plays a key role in antibody transport. The binding site on Fc
for FcRn is also the site at which the bacterial proteins A and G
bind. The tight binding by these proteins is typically exploited as
a means to purify antibodies by employing protein A or protein G
affinity chromatography during protein purification. Thus the
fidelity of this region on Fc is important for both the clinical
properties of antibodies and their purification. Available
structures of the rat Fc/FcRn complex (Burmeister et al., 1994,
Nature, 372:379-383; Martin et al., 2001, Mol Cell 7:867-877, both
entirely incorporated by reference), and of the complexes of Fc
with proteins A and G (Deisenhofer, 1981, Biochemistry
20:2361-2370; Sauer-Eriksson et al., 1995, Structure 3:265-278;
Tashiro et al., 1995, Curr Opin Struct Biol 5:471-481, all entirely
incorporated by reference), provide insight into the interaction of
Fc with these proteins. The FcRn receptor is also responsible for
the transfer of IgG to the neo-natal gut and to the lumen of the
intestinal epithelia in adults (Ghetie and Ward, Annu. Rev.
Immunol., 2000, 18:739-766; Yoshida et al., Immunity, 2004,
20(6):769-783, both entirely incorporated by reference).
[0005] Studies of rat and human Fc domains have demonstrated the
importance of some Fc residues to the binding of FcRn. The rat and
human sequences have about 64% sequence identity in the Fc regions
(residues 237-443 in the numbering of EU index). See FIGS. 3, 4,
and 5 for the rat/human alignments of Fc, FcRn heavy chain, and
FcRn light chain (beta-2-microglobulin). A model of the human
Fc/FcRn complex has been built from the existing structure of the
rat Fc/FcRn complex (Martin et al., 2001, Mol Cell 7:867-877,
entirely incorporated by reference). The rat and human sequences
share some residues that are critical for FcRn binding, such as
H310 and H435 (Medesan et al., 1997 J. Immunol. 158(5):221-7;
Shields et al., 2001, J. Biol. Chem. 276(9):6591-6604, both
entirely incorporated by reference). In many positions, however,
the human and rat proteins have different amino acids, giving the
residues in the human sequence different environments, and possibly
a different identities, than in the rat sequence. This variability
limits the ability to transfer characteristics from one homolog to
the other homolog.
[0006] In the murine Fc, random mutation and phage display
selection at the sites, T252, T254, and T256 lead to a triple
mutant, T252L/T254S/T256F, that has a 3.5-fold increase in FcRn
affinity and a 1.5-fold increase in serum half-life (Ghetie et al.,
1997, Nat. Biotech. 15(7): 637-640, entirely incorporated by
reference). Disruption of the Fc/FcRn interaction by mutations at
positions 253, 310 and 435 also lead to decreased in vivo half-life
(Medesan et al J. Immunol. 1997 158(5):2211-7, entirely
incorporated by reference).
[0007] Mutational studies in human Fc.gamma. have been done on some
of the residues that are important for binding to FcRn and have
demonstrated an increased serum half-life. In human Fc.gamma.1,
Hinton et al. mutated three residues individually to the other 19
common amino acids. Hinton et al., found that some point mutants a
double mutant increased the FcRn binding affinity (Hinton et al.,
2004, J. Biol. Chem. 279(8): 6213-6216; Hinton et al. Journal of
Immunology 2006, 176:346-356, both entirely incorporated by
reference). Two mutations had increased half-lives in monkeys.
Shields et al. mutated residues, almost exclusively to Ala, and
studied their binding to FcRn and the Fc.gamma.R's (Shields et al.,
2001, J. Biol. Chem., 276(9):6591-6604, entirely incorporated by
reference).
[0008] Dall'Acqua et al. used phage display to select for Fc
mutations that bound FcRn with increased affinity (Dall' Acqua et
al. 2002, J. Immunol. 169:5171-5180, entirely incorporated by
reference). The DNA sequences selected for were primarily double
and triple mutants. The reference expressed the proteins encoded by
many of their selected sequences and found some that bound to FcRn
more tightly than the wild-type Fc.
[0009] The administration of antibodies and Fc fusion proteins as
therapeutics requires injections with a prescribed frequency
relating to the clearance and half-life characteristics of the
protein. Longer in vivo half-lives allow more seldom injections or
lower dosing, which is clearly advantageous. Although the past
mutations in the Fc domain have lead to some proteins with
increased FcRn binding affinity and in vivo half-lives, these
mutations have not identified the optimal mutations and enhanced in
vivo half-life.
[0010] One feature of the Fc region is the conserved N-linked
glycosylation that occurs at N297. This carbohydrate, or
oligosaccharide as it is sometimes called, plays a critical
structural and functional role for the antibody, and is one of the
principle reasons that antibodies must be produced using mammalian
expression systems. Umana et al., 1999, Nat Biotechnol 17:176-180;
Davies et al., 2001, Biotechnol Bioeng 74:288-294; Mimura et al.,
2001, J Biol Chem 276:45539-45547.; Radaev et al., 2001, J Biol
Chem 276:16478-16483; Shields et al., 2001, J Biol Chem
276:6591-6604; Shields et al., 2002, J Biol Chem 277:26733-26740;
Simmons et al., 2002, J Immunol Methods 263:133-147; Radaev et al.,
2001, J Biol Chem 276:16469-16477; and Krapp et al., 2003, J Mol
Biol 325:979-989, all entirely incorporated by reference).
[0011] Antibodies have been developed for therapeutic use.
Representative publications related to such therapies include
Chamow et al., 1996, Trends Biotechnol 14:52-60; Ashkenazi et al.,
1997, Curr Opin Immunol 9:195-200, Cragg et al., 1999, Curr Opin
Immunol 11:541-547; Glennie et al., 2000, Immunol Today 21:403-410,
McLaughlin et al., 1998, J Clin Oncol 16:2825-2833, and Cobleigh et
al., 1999, J Clin Oncol 17:2639-2648, all entirely incorporated by
reference. Currently for anticancer therapy, any small improvement
in mortality rate defines success. Certain IgG variants disclosed
herein enhance the capacity of antibodies to limit further growth
or destroy at least partially, targeted cancer cells.
[0012] Anti-tumor potency of antibodies is via enhancement of their
ability to mediate cytotoxic effector functions such as ADCC, ADCP,
and CDC. Examples include Clynes et al., 1998, Proc Natl Acad Sci
USA 95:652-656; Clynes et al., 2000, Nat Med 6:443-446 and Cartron
et al., 2002, Blood 99:754-758, both entirely incorporated by
reference.
[0013] Human IgG1 is the most commonly used antibody for
therapeutic purposes, and the majority of engineering studies have
been constructed in this context. The different isotypes of the IgG
class however, including IgG1, IgG2, IgG3, and IgG4, have unique
physical, biological, and clinical properties. There is a need in
the art to design improved IgG1, IgG2, IgG3, and IgG4 variants.
There is a further need to design such variants to improve binding
to FcRn and/or increase in vivo half-life as compared to native IgG
polypeptides. Additionally, there is a need to combine variants
with improved pharmacokinetic properties with variants comprising
modifications to improve efficacy through altered FcgammaR binding.
The present application meets these and other needs.
SUMMARY OF THE INVENTION
[0014] The present application is directed to Fc variants of a
parent polypeptide including at least one modification in the Fc
region of the polypeptide. In various embodiments, the variant
polypeptides exhibit altered binding to FcRn as compared to a
parent polypeptide. In certain variations, the modification can be
selected from the group consisting of: 428L, 434M and 434S, where
the numbering is according to the EU Index in Kabat et al.
[0015] In another embodiment, the Fc variant includes at least two
modifications selected from the group consisting of: 252Y/428L,
428L/434H, 428L/434F, 428L/434Y, 428L/434A, 428L/434M, and
428L/434S.
[0016] In another embodiment, the Fc variant includes at least one
modification selected from the group consisting of: M428L/N434S,
V308F/M428L/N434S.
[0017] In another embodiment, the Fc variant includes at least one
modification selected from the group consisting of: 259I/434S,
308F/434S, 308F/428L/434S, 259I/308F/434S, 307Q/308F/434S,
2501/308F/434S, and 308F1319L/434S.
[0018] In another embodiment, the Fc variant includes at least one
modification selected from the group consisting of:
[0019] In another embodiment, the invention includes a method of
treating a patient in need of said treatment comprising
administering an effective amount of an Fc variant described
herein.
[0020] In another embodiment, the invention includes a method of
increasing the half-life of an antibody or immunoadhesin by
modifying an Fc according to the modifications described
herein.
[0021] In another variant, the invention includes Fc variant with
enchanced FcRn binding with additional Fc variants that modulate
effector function.
BRIEF DESCRIPTION OF THE DRAWINGS
[0022] FIG. 1. Sequence alignments of human IgG constant heavy
chains. Gray indicates differences from IgG1, and boxed residues
indicate common allotypic variations in the human population.
[0023] FIG. 2. (SEQ ID NO: 1-6) Amino acid sequences of constant
regions used in the invention.
[0024] FIG. 3. (SEQ ID NO: 7-12) Amino acid sequences of exemplary
variant constant regions.
[0025] FIG. 4. (SEQ ID NO: 13-22) Amino acid sequences of VH and VL
variable regions used in the invention.
[0026] FIG. 5. (SEQ ID NO: 23-29) Amino acid sequences of exemplary
variant antibodies.
[0027] FIG. 6. Relative VEGF binding by WT and select variant IgG1
anti-VEGF antibodies. The plot shows the Biacore response units
(RU) at the end of the association phase for binding of antibody
analyte to immobilized VEGF antigen. Anti-Her2 IgG1 antibody was
used as a negative control.
[0028] FIG. 7. Biacore sensorgrams of WT and variant IgG1
antibodies to immobilized human FcRn at low (6.0) and high (7.4)
pH.
[0029] FIG. 8. FcRn binding affinities of WT and select variant
IgG1 antibodies to human FcRn at pH 6.0 as determined by Biacore.
The graph shows a plot of the pseudo-affinity constant (Ka*), on a
log scale.
[0030] FIG. 9. Relative binding of variant IgG1 anti-VEGF
antibodies to human FcRn as determined by Biacore. The table shows
the fold of the Ka* of each variant relative to human WT (native)
IgG1. n indicates the number of time each variant was tested, and
Mean and SD indicate the average and standard deviation
respectively for each variant over n binding experiments. Fold FcRn
was calculated for all variants relative to WT IgG1 within each
respective binding experiment. NB indicates no binding was
detected. ND indicates that binding was not determined for that
particular variant. NF indicates no fit was possible from the
binding data.
[0031] FIG. 10. Relative binding of variant IgG2 and IgG1/2
anti-VEGF antibodies to human FcRn as determined by Biacore. The
table is as described in FIG. 9.
[0032] FIG. 11a-11c. Analysis of additive and synergistic
substitution combinations. FIG. 11a shows a plot of the
experimentally determined fold binding to human FcRn by each
variant versus the predicted fold FcRn binding as determined by the
product of the single variants. Variant data points are labeled,
and the line represents perfect additivity. FIG. 11b shows the
difference between experimental and predicted fold for each
combination variant. FIG. 11c shows the synergy of each variant
combination. % synergy is calculated as the
100.times.[(experimental fold/predicted fold)-1)].
[0033] FIG. 12a-12d. Relative binding of variant anti-TNF, -CD25,
-EGFR, and -IgE antibodies to human FcRn as determined by Biacore.
The table is as described in FIG. 9.
[0034] FIG. 13a-13b. In vivo pharmacokinetics of WT and variant
antibodies in mFcRn-/- hFcRn+ mice. The graphs plot the serum
concentration of antibody versus time after a single intravenous
dose. FIG. 13a shows data from one of the 4 studies carried out
with IgG1 antibodies (Study 3), and FIG. 13b shows data from a
study carried out with IgG2 antibodies (Study 5).
[0035] FIG. 14. Fitted PK parameters from all in vivo PK studies
carried out in mFcRn-/- hFcRn+ mice with variant and WT antibodies.
n represents the number of mice per group, with Mean and standard
deviation (SD) data provided for PK parameters. Half-Life
represents the beta phase that characterizes elimination of
antibody from serum. Cmax is the maximal observed serum
concentration, AUC is the area under the concetration time curve,
and clearance is the clearance of antibody from serum. Fold
half-life is calculated as the half-life of variant antibody over
that of the WT IgG1 or IgG2 parent within each study.
[0036] FIG. 15a-15c. Correlation between half-life of IgG1 (FIG.
15a) and IgG2 (FIG. 15b) variant antibodies in mFcRn-/- hFcRn+ mice
and fold FcRn binding relative to WT IgG1. Data on the y-axis are
from FIG. 14, and data on the x-axis are from FIGS. 9 and 10.
Select variants are labeled, and variant data from repeat
experiments are circled. FIG. 15c shows both IgG1 and IgG2
correlation data, with the black and gray lines representing fits
of the IgG1 and IgG2 data respectively.
[0037] FIG. 16. (SEQ ID NO: 30-35) Amino acid sequences of variant
and parent anti-TNF Fc immunoadhesins used in the invention.
[0038] FIG. 17. Binding of anti-TNF immunoadhesins to TNF antigen
as determined by Biacore.
[0039] FIG. 18. Relative binding of variant Fc immunoadhesins to
human FcRn as determined by Biacore. The table shows the fold of
the Ka* of each variant relative to human WT (native) IgG1. n
indicates the number of time each variant was tested, and Mean and
SD indicate the average and standard deviation respectively for
each variant over n binding experiments. Fold FcRn was calculated
for all variants relative to the respective IgG parent within each
respective binding experiment.
[0040] FIG. 19. In vivo pharmacokinetics of parent and variant Fc
immunoadhesins in mFcRn-/- hFcRn+ mice. The graphs plot the serum
concentration of Fc fusion versus time after a single intravenous
dose.
[0041] FIG. 20. Fitted PK parameters from the Fc fusion in vivo PK
study in mFcRn-/- hFcRn+ mice. Parameters are as described in FIG.
14. % increase in half-life is calculated as 100 times the
half-life of variant Fc fusion over that of the WT IgG1 or IgG2
parent.
[0042] FIG. 21a-21b. Relative binding of variant IgG1 anti-VEGF
antibodies to cynomolgus monkey and human FcRn as determined by
Biacore. FIG. 21a shows the data in tabular form. Description of
the figure is as in FIG. 9, and data for binding to human FcRn are
taken from FIG. 9. FIG. 21 b shows a plot of the data.
[0043] FIG. 22. In vivo pharmacokinetics of WT and variant
antibodies in cynomolgus monkeys. The graphs plot the serum
concentration of antibody versus time after a single intravenous
dose.
[0044] FIG. 23. Fitted PK parameters from the in vivo PK study in
cynomolgus monkeys with variant and WT antibodies. Parameters are
as described in FIG. 14.
DETAILED DESCRIPTION OF THE INVENTION
[0045] The present invention discloses the generation of novel
variants of Fc domains, including those found in antibodies, Fc
fusions, and immuno-adhesions, which have an increased binding to
the FcRn receptor. As noted herein, binding to FcRn results in
longer serum retention in vivo.
[0046] In order to increase the retention of the Fc proteins in
vivo, the increase in binding affinity must be at around pH 6 while
maintaining lower affinity at around pH 7.4. Although still under
examination, Fc regions are believed to have longer half-lives in
vivo, because binding to FcRn at pH 6 in an endosome sequesters the
Fc (Ghetie and Ward, 1997 Immunol Today. 18(12): 592-598, entirely
incorporated by reference). The endosomal compartment then recycles
the Fc to the cell surface. Once the compartment opens to the
extracellular space, the higher pH, .about.7.4, induces the release
of Fc back into the blood. In mice, Dall' Acqua et al. showed that
Fc mutants with increased FcRn binding at pH 6 and pH 7.4 actually
had reduced serum concentrations and the same half life as
wild-type Fc (Dall' Acqua et al. 2002, J. Immunol. 169:5171-5180,
entirely incorporated by reference). The increased affinity of Fc
for FcRn at pH 7.4 is thought to forbid the release of the Fc back
into the blood. Therefore, the Fc mutations that will increase Fc's
half-life in vivo will ideally increase FcRn binding at the lower
pH while still allowing release of Fc at higher pH. The amino acid
histidine changes its charge state in the pH range of 6.0 to 7.4.
Therefore, it is not surprising to find His residues at important
positions in the Fc/FcRn complex (FIG. 6.)
[0047] An additional aspect of the invention is the increase in
FcRn binding over wild type specifically at lower pH, about pH 6.0,
to facilitate Fc/FcRn binding in the endosome. Also disclosed are
Fc variants with altered FcRn binding and altered binding to
another class of Fc receptors, the Fc.gamma.R's (sometimes written
FcgammaR's) as differential binding to Fc.gamma.Rs, particularly
increased binding to Fc.gamma.RIIIb and decreased binding to
Fc.gamma.RIIb, has been shown to result in increased efficacy.
[0048] Definitions
[0049] In order that the application may be more completely
understood, several definitions are set forth below. Such
definitions are meant to encompass grammatical equivalents.
[0050] By "ADCC" or "antibody dependent cell-mediated cytotoxicity"
as used herein is meant the cell-mediated reaction wherein
nonspecific cytotoxic cells that express Fc.gamma.Rs recognize
bound antibody on a target cell and subsequently cause lysis of the
target cell.
[0051] By "ADCP" or antibody dependent cell-mediated phagocytosis
as used herein is meant the cell-mediated reaction wherein
nonspecific cytotoxic cells that express Fc.gamma.Rs recognize
bound antibody on a target cell and subsequently cause phagocytosis
of the target cell.
[0052] By "modification" herein is meant an amino acid
substitution, insertion, and/or deletion in a polypeptide sequence
or an alteration to a moiety chemically linked to a protein. For
example, a modification may be an altered carbohydrate or PEG
structure attached to a protein. By "amino acid modification"
herein is meant an amino acid substitution, insertion, and/or
deletion in a polypeptide sequence.
[0053] By "amino acid substitution" or "substitution" herein is
meant the replacement of an amino acid at a particular position in
a parent polypeptide sequence with another amino acid. For example,
the substitution E272Y refers to a variant polypeptide, in this
case an Fc variant, in which the glutamic acid at position 272 is
replaced with tyrosine.
[0054] By "amino acid insertion" or "insertion" as used herein is
meant the addition of an amino acid sequence at a particular
position in a parent polypeptide sequence. For example, -233E or
A233E designates an insertion of glutamic acid after position 233
and before position 234. Additionally, -233ADE or 233ADE designates
an insertion of AlaAspGlu after position 233 and before position
234.
[0055] By "amino acid deletion" or "deletion" as used herein is
meant the removal of an amino acid sequence at a particular
position in a parent polypeptide sequence. For example, E233- or
E233# designates a deletion of glutamic acid at position 233.
Additionally, EDA233- or EDA233# designates a deletion of the
sequence GluAspAla that begins at position 233.
[0056] By "variant protein" or "protein variant", or "variant" as
used herein is meant a protein that differs from that of a parent
protein by virtue of at least one amino acid modification. Protein
variant may refer to the protein itself, a composition comprising
the protein, or the amino sequence that encodes it. Preferably, the
protein variant has at least one amino acid modification compared
to the parent protein, e.g. from about one to about seventy amino
acid modifications, and preferably from about one to about five
amino acid modifications compared to the parent. The protein
variant sequence herein will preferably possess at least about 80%
homology with a parent protein sequence, and most preferably at
least about 90% homology, more preferably at least about 95%
homology. Variant protein can refer to the variant protein itself,
compositions comprising the protein variant, or the DNA sequence
that encodes it. Accordingly, by "antibody variant" or "variant
antibody" as used herein is meant an antibody that differs from a
parent antibody by virtue of at least one amino acid modification,
"IgG variant" or "variant IgG" as used herein is meant an antibody
that differs from a parent IgG by virtue of at least one amino acid
modification, and "immunoglobulin variant" or "variant
immunoglobulin" as used herein is meant an immunoglobulin sequence
that differs from that of a parent immunoglobulin sequence by
virtue of at least one amino acid modification. "Fc variant" or
"variant Fc" as used herein is meant a protein comprising a
modification in an Fc domain. The Fc variants of the present
invention are defined according to the amino acid modifications
that compose them. Thus, for example, N434S or 434S is an Fc
variant with the substitution serine at position 434 relative to
the parent Fc polypeptide, wherein the numbering is according to
the EU index. Likewise, M428L/N434S defines an Fc variant with the
substitutions M428L and N434S. A relative to the parent Fc
polypeptide. The identity of the WT amino acid may be unspecified,
in which case the aforementioned variant is referred to as
428L/434S. It is noted that the order in which substitutions are
provided is arbitrary, that is to say that, for example, 428L/434S
is the same Fc variant as M428L/N434S, and so on. For all positions
discussed in the present invention, numbering is according to the
EU index. The EU index or EU index as in Kabat or EU numbering
scheme refers to the numbering of the EU antibody (Edelman et al.,
1969, Proc Natl Acad Sci USA 63:78-85, hereby entirely incorporated
by reference.) The modification can be an addition, deletion, or
substitution. Substitutions can include naturally occurring amino
acids and non-naturally occurring amino acids. Variants may
comprise non-natural amino acids. Examples include U.S. Pat. No.
6,586,207; WO 98/48032; WO 03/073238; US2004-0214988A1; WO
05/35727A2; WO 05/74524A2; J. W. Chin et al., (2002), Journal of
the American Chemical Society 124:9026-9027; J. W. Chin, & P.
G. Schultz, (2002), ChemBioChem 11:1135-1137; J. W. Chin, et al.,
(2002), PICAS United States of America 99:11020-11024; and, L.
Wang, & P. G. Schultz, (2002), Chem. 1-10, all entirely
incorporated by reference.
[0057] As used herein, "protein" herein is meant at least two
covalently attached amino acids, which includes proteins,
polypeptides, oligopeptides and peptides. The peptidyl group may
comprise naturally occurring amino acids and peptide bonds, or
synthetic peptidomimetic structures, i.e. "analogs", such as
peptoids (see Simon et al., PNAS USA 89(20):9367 (1992), entirely
incorporated by reference). The amino acids may either be naturally
occurring or non-naturally occurring; as will be appreciated by
those in the art. For example, homo-phenylalanine, citrulline, and
noreleucine are considered amino acids for the purposes of the
invention, and both D- and L- (R or S) configured amino acids may
be utilized. The variants of the present invention may comprise
modifications that include the use of unnatural amino acids
incorporated using, for example, the technologies developed by
Schultz and colleagues, including but not limited to methods
described by Cropp & Shultz, 2004, Trends Genet. 20(12):625-30,
Anderson et al., 2004, Proc Natl Acad Sci USA 101(2):7566-71, Zhang
et al., 2003, 303(5656):371-3, and Chin et al., 2003, Science
301(5635):964-7, all entirely incorporated by reference. In
addition, polypeptides may include synthetic derivatization of one
or more side chains or termini, glycosylation, PEGylation, circular
permutation, cyclization, linkers to other molecules, fusion to
proteins or protein domains, and addition of peptide tags or
labels.
[0058] By "residue" as used herein is meant a position in a protein
and its associated amino acid identity. For example, Asparagine 297
(also referred to as Asn297 or N297) is a residue at position 297
in the human antibody IgG1.
[0059] By "Fab" or "Fab region" as used herein is meant the
polypeptide that comprises the VH, CH1, VL, and CL immunoglobulin
domains. Fab may refer to this region in isolation, or this region
in the context of a full length antibody, antibody fragment or Fab
fusion protein. By "Fv" or "Fv fragment" or "Fv region" as used
herein is meant a polypeptide that comprises the VL and VH domains
of a single antibody.
[0060] By "IgG subclass modification" as used herein is meant an
amino acid modification that converts one amino acid of one IgG
isotype to the corresponding amino acid in a different, aligned IgG
isotype. For example, because IgG1 comprises a tyrosine and IgG2 a
phenylalanine at EU position 296, a F296Y substitution in IgG2 is
considered an IgG subclass modification.
[0061] By "non-naturally occurring modification" as used herein is
meant an amino acid modification that is not isotypic. For example,
because none of the IgGs comprise a serine at position 434, the
substitution 434S in IgG1, IgG2, IgG3, or IgG4 is considered a
non-naturally occuring modification.
[0062] By "amino acid" and "amino acid identity" as used herein is
meant one of the 20 naturally occurring amino acids or any
non-natural analogues that may be present at a specific, defined
position.
[0063] By "effector function" as used herein is meant a biochemical
event that results from the interaction of an antibody Fc region
with an Fc receptor or ligand. Effector functions include but are
not limited to ADCC, ADCP, and CDC.
[0064] By "IgG Fc ligand" as used herein is meant a molecule,
preferably a polypeptide, from any organism that binds to the Fc
region of an IgG antibody to form an Fc/Fc ligand complex. Fc
ligands include but are not limited to Fc.gamma.Rs, Fc.gamma.Rs,
Fc.gamma.Rs, FcRn, C1q, C3, mannan binding lectin, mannose
receptor, staphylococcal protein A, streptococcal protein G, and
viral Fc.gamma.R. Fc ligands also include Fc receptor homologs
(FcRH), which are a family of Fc receptors that are homologous to
the Fc.gamma.Rs (Davis et al., 2002, Immunological Reviews
190:123-136, entirely incorporated by reference). Fc ligands may
include undiscovered molecules that bind Fc. Particular IgG Fc
ligands are FcRn and Fc gamma receptors. By "Fc ligand" as used
herein is meant a molecule, preferably a polypeptide, from any
organism that binds to the Fc region of an antibody to form an
Fc/Fc ligand complex.
[0065] By "Fc gamma receptor", "Fc.gamma.R" or "FcgammaR" as used
herein is meant any member of the family of proteins that bind the
IgG antibody Fc region and is encoded by an Fc.gamma.R gene. In
humans this family includes but is not limited to Fc.gamma.RI
(CD64), including isoforms Fc.gamma.RIa, Fc.gamma.RIb, and
Fc.gamma.RIc; Fc.gamma.RII (CD32), including isoforms Fc.gamma.RIIa
(including allotypes H131 and R131), Fc.gamma.RIIb (including
Fc.gamma.RIIb-1 and Fc.gamma.RIIb-2), and Fc.gamma.RIIc; and
Fc.gamma.RIII (CD16), including isoforms Fc.gamma.RIIIa (including
allotypes V158 and F158) and Fc.gamma.RIIIb (including allotypes
Fc.gamma.RIIIb-NA1 and Fc.gamma.RIIIb-NA2) (Jefferis et al., 2002,
Immunol Lett 82:57-65, entirely incorporated by reference), as well
as any undiscovered human Fc.gamma.Rs or Fc.gamma.R isoforms or
allotypes. An Fc.gamma.R may be from any organism, including but
not limited to humans, mice, rats, rabbits, and monkeys. Mouse
Fc.gamma.Rs include but are not limited to Fc.gamma.RI (CD64),
Fc.gamma.RII (CD32), Fc.gamma.RIII (CD16), and Fc.gamma.RIII-2
(CD16-2), as well as any undiscovered mouse Fc.gamma.Rs or
Fc.gamma.R isoforms or allotypes.
[0066] By "FcRn" or "neonatal Fc Receptor" as used herein is meant
a protein that binds the IgG antibody Fc region and is encoded at
least in part by an FcRn gene. The FcRn may be from any organism,
including but not limited to humans, mice, rats, rabbits, and
monkeys. As is known in the art, the functional FcRn protein
comprises two polypeptides, often referred to as the heavy chain
and light chain. The light chain is beta-2-microglobulin and the
heavy chain is encoded by the FcRn gene. Unless other wise noted
herein, FcRn or an FcRn protein refers to the complex of FcRn heavy
chain with beta-2-microglobulin. Sequences of particular interest
of FcRn are shown in the Figures, particularly the human
species.
[0067] By "parent polypeptide" as used herein is meant an
unmodified polypeptide that is subsequently modified to generate a
variant. The parent polypeptide may be a naturally occurring
polypeptide, or a variant or engineered version of a naturally
occurring polypeptide. Parent polypeptide may refer to the
polypeptide itself, compositions that comprise the parent
polypeptide, or the amino acid sequence that encodes it.
Accordingly, by "parent immunoglobulin" as used herein is meant an
unmodified immunoglobulin polypeptide that is modified to generate
a variant, and by "parent antibody" as used herein is meant an
unmodified antibody that is modified to generate a variant
antibody. It should be noted that "parent antibody" includes known
commercial, recombinantly produced antibodies as outlined
below.
[0068] By "position" as used herein is meant a location in the
sequence of a protein. Positions may be numbered sequentially, or
according to an established format, for example the EU index for
antibody numbering.
[0069] By "target antigen" as used herein is meant the molecule
that is bound specifically by the variable region of a given
antibody. A target antigen may be a protein, carbohydrate, lipid,
or other chemical compound.
[0070] By "target cell" as used herein is meant a cell that
expresses a target antigen.
[0071] By "variable region" as used herein is meant the region of
an immunoglobulin that comprises one or more Ig domains
substantially encoded by any of the V.kappa., V.lamda., and/or VH
genes that make up the kappa, lambda, and heavy chain
immunoglobulin genetic loci respectively.
[0072] By "wild type or WT" herein is meant an amino acid sequence
or a nucleotide sequence that is found in nature, including allelic
variations. A WT protein has an amino acid sequence or a nucleotide
sequence that has not been intentionally modified.
[0073] The present invention is directed to antibodies that exhibit
increased binding to FcRn relative to a wild-type antibody. For
example, in some instances, increased binding results in cellular
recycling of the antibody and hence increased half-life. In
addition, antibodies exhibiting increased binding to FcRn and
altered binding to other Fc receptors, eg. Fc.gamma.Rs, find use in
the present invention.
Antibodies
[0074] The present application is directed to antibodies that
include amino acid modifications that modulate binding to FcRn. Of
particular interest are antibodies that minimally comprise an Fc
region, or functional variant thereof, that displays increased
binding affinity to FcRn at lowered pH, and do not exhibit
substantially altered binding at higher pH.
[0075] Traditional antibody structural units typically comprise a
tetramer. Each tetramer is typically composed of two identical
pairs of polypeptide chains, each pair having one "light"
(typically having a molecular weight of about 25 kDa) and one
"heavy" chain (typically having a molecular weight of about 50-70
kDa). Human light chains are classified as kappa and lambda light
chains. Heavy chains are classified as mu, delta, gamma, alpha, or
epsilon, and define the antibody's isotype as IgM, IgD, IgG, IgA,
and IgE, respectively. IgG has several subclasses, including, but
not limited to IgG1, IgG2, IgG3, and IgG4. IgM has subclasses,
including, but not limited to, IgM1 and IgM2. Thus, "isotype" as
used herein is meant any of the subclasses of immunoglobulins
defined by the chemical and antigenic characteristics of their
constant regions. The known human immunoglobulin isotypes are IgG1,
IgG2, IgG3, IgG4, IgA1, IgA2, IgM1, IgM2, IgD, and IgE.
[0076] The amino-terminal portion of each chain includes a variable
region of about 100 to 110 or more amino acids primarily
responsible for antigen recognition. In the variable region, three
loops are gathered for each of the V domains of the heavy chain and
light chain to form an antigen-binding site. Each of the loops is
referred to as a complementarity-determining region (hereinafter
referred to as a "CDR"), in which the variation in the amino acid
sequence is most significant.
[0077] The carboxy-terminal portion of each chain defines a
constant region primarily responsible for effector function. Kabat
et al. collected numerous primary sequences of the variable regions
of heavy chains and light chains. Based on the degree of
conservation of the sequences, they classified individual primary
sequences into the CDR and the framework and made a list thereof
(see SEQUENCES OF IMMUNOLOGICAL INTEREST, 5th edition, NIH
publication, No. 91-3242, E. A. Kabat et al., entirely incorporated
by reference).
[0078] In the IgG subclass of immunoglobulins, there are several
immunoglobulin domains in the heavy chain. By "immunoglobulin (Ig)
domain" herein is meant a region of an immunoglobulin having a
distinct tertiary structure. Of interest in the present invention
are the heavy chain domains, including, the constant heavy (CH)
domains and the hinge domains. In the context of IgG antibodies,
the IgG isotypes each have three CH regions. Accordingly, "CH"
domains in the context of IgG are as follows: "CH1" refers to
positions 118-220 according to the EU index as in Kabat. "CH2"
refers to positions 237-340 according to the EU index as in Kabat,
and "CH3" refers to positions 341-447 according to the EU index as
in Kabat.
[0079] Another type of Ig domain of the heavy chain is the hinge
region. By "hinge" or "hinge region" or "antibody hinge region" or
"immunoglobulin hinge region" herein is meant the flexible
polypeptide comprising the amino acids between the first and second
constant domains of an antibody. Structurally, the IgG CH1 domain
ends at EU position 220, and the IgG CH2 domain begins at residue
EU position 237. Thus for IgG the antibody hinge is herein defined
to include positions 221 (D221 in IgG1) to 236 (G236 in IgG1),
wherein the numbering is according to the EU index as in Kabat. In
some embodiments, for example in the context of an Fc region, the
lower hinge is included, with the "lower hinge" generally referring
to positions 226 or 230.
[0080] Of particular interest in the present invention are the Fc
regions. By "Fc" or "Fc region", as used herein is meant the
polypeptide comprising the constant region of an antibody excluding
the first constant region immunoglobulin domain and in some cases,
part of the hinge. Thus Fc refers to the last two constant region
immunoglobulin domains of IgA, IgD, and IgG, the last three
constant region immunoglobulin domains of IgE and IgM, and the
flexible hinge N-terminal to these domains. For IgA and IgM, Fc may
include the J chain. For IgG, as illustrated in FIG. 1, Fc
comprises immunoglobulin domains Cgamma2 and Cgamma3 (Cg2 and Cg3)
and the lower hinge region between Cgamma1 (Cg1) and Cgamma2 (Cg2).
Although the boundaries of the Fc region may vary, the human IgG
heavy chain Fc region is usually defined to include residues C226
or P230 to its carboxyl-terminus, wherein the numbering is
according to the EU index as in Kabat. Fc may refer to this region
in isolation, or this region in the context of an Fc polypeptide,
as described below. By "Fc polypeptide" as used herein is meant a
polypeptide that comprises all or part of an Fc region. Fc
polypeptides include antibodies, Fc fusions, isolated Fcs, and Fc
fragments.
[0081] In some embodiments, the antibodies are full length. By
"full length antibody" herein is meant the structure that
constitutes the natural biological form of an antibody, including
variable and constant regions, including one or more modifications
as outlined herein.
[0082] Alternatively, the antibodies can be a variety of
structures, including, but not limited to, antibody fragments,
monoclonal antibodies, bispecific antibodies, minibodies, domain
antibodies, synthetic antibodies (sometimes referred to herein as
"antibody mimetics"), chimeric antibodies, humanized antibodies,
antibody fusions (sometimes referred to as "antibody conjugates"),
and fragments of each, respectively.
[0083] Antibody Fragments
[0084] In one embodiment, the antibody is an antibody fragment. Of
particular interest are antibodies that comprise Fc regions, Fc
fusions, and the constant region of the heavy chain
(CH1-hinge-CH2--CH3), again also including constant heavy region
fusions.
[0085] Specific antibody fragments include, but are not limited to,
(i) the Fab fragment consisting of VL, VH, CL and CH1 domains, (ii)
the Fd fragment consisting of the VH and CH1 domains, (iii) the Fv
fragment consisting of the VL and VH domains of a single antibody;
(iv) the dAb fragment (Ward et al., 1989, Nature 341:544-546,
entirely incorporated by reference) which consists of a single
variable, (v) isolated CDR regions, (vi) F(ab')2 fragments, a
bivalent fragment comprising two linked Fab fragments (vii) single
chain Fv molecules (scFv), wherein a VH domain and a VL domain are
linked by a peptide linker which allows the two domains to
associate to form an antigen binding site (Bird et al., 1988,
Science 242:423-426, Huston et al., 1988, Proc. Natl. Acad. Sci.
U.S.A. 85:5879-5883, entirely incorporated by reference), (viii)
bispecific single chain Fv (WO 03/11161, hereby incorporated by
reference) and (ix) "diabodies" or "triabodies", multivalent or
multispecific fragments constructed by gene fusion (Tomlinson et.
al., 2000, Methods Enzymol. 326:461-479; WO94/13804; Holliger et
al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:6444-6448, all entirely
incorporated by reference). The antibody fragments may be modified.
For example, the molecules may be stabilized by the incorporation
of disulphide bridges linking the VH and VL domains (Reiter et al.,
1996, Nature Biotech. 14:1239-1245, entirely incorporated by
reference).
[0086] Chimeric and Humanized Antibodies
[0087] In some embodiments, the scaffold components can be a
mixture from different species. As such, if the protein is an
antibody, such antibody may be a chimeric antibody and/or a
humanized antibody. In general, both "chimeric antibodies" and
"humanized antibodies" refer to antibodies that combine regions
from more than one species. For example, "chimeric antibodies"
traditionally comprise variable region(s) from a mouse (or rat, in
some cases) and the constant region(s) from a human. "Humanized
antibodies" generally refer to non-human antibodies that have had
the variable-domain framework regions swapped for sequences found
in human antibodies. Generally, in a humanized antibody, the entire
antibody, except the CDRs, is encoded by a polynucleotide of human
origin or is identical to such an antibody except within its CDRs.
The CDRs, some or all of which are encoded by nucleic acids
originating in a non-human organism, are grafted into the
beta-sheet framework of a human antibody variable region to create
an antibody, the specificity of which is determined by the
engrafted CDRs. The creation of such antibodies is described in,
e.g., WO 92/11018, Jones, 1986, Nature 321:522-525, Verhoeyen et
al., 1988, Science 239:1534-1536, all entirely incorporated by
reference. "Backmutation" of selected acceptor framework residues
to the corresponding donor residues is often required to regain
affinity that is lost in the initial grafted construct (U.S. Pat.
No. 5,530,101; U.S. Pat. No. 5,585,089; U.S. Pat. No. 5,693,761;
U.S. Pat. No. 5,693,762; U.S. Pat. No. 6,180,370; U.S. Pat. No.
5,859,205; U.S. Pat. No. 5,821,337; U.S. Pat. No. 6,054,297; U.S.
Pat. No. 6,407,213, all entirely incorporated by reference). The
humanized antibody optimally also will comprise at least a portion
of an immunoglobulin constant region, typically that of a human
immunoglobulin, and thus will typically comprise a human Fc region.
Humanized antibodies can also be generated using mice with a
genetically engineered immune system. Roque et al., 2004,
Biotechnol. Prog. 20:639-654, entirely incorporated by reference. A
variety of techniques and methods for humanizing and reshaping
non-human antibodies are well known in the art (See Tsurushita
& Vasquez, 2004, Humanization of Monoclonal Antibodies,
Molecular Biology of B Cells, 533-545, Elsevier Science (USA), and
references cited therein, all entirely incorporated by reference).
Humanization methods include but are not limited to methods
described in Jones et al., 1986, Nature 321:522-525; Riechmann et
al., 1988; Nature 332:323-329; Verhoeyen et al., 1988, Science,
239:1534-1536; Queen et al., 1989, Proc Natl Acad Sci, USA
86:10029-33; He et al., 1998, J. Immunol. 160: 1029-1035; Carter et
al., 1992, Proc Natl Acad Sci USA 89:4285-9, Presta et al., 1997,
Cancer Res.57(20):4593-9; Gorman et al., 1991, Proc. Natl. Acad.
Sci. USA 88:4181-4185; O'Connor et al., 1998, Protein Eng 11:321-8,
all entirely incorporated by reference. Humanization or other
methods of reducing the immunogenicity of nonhuman antibody
variable regions may include resurfacing methods, as described for
example in Roguska et al., 1994, Proc. Natl. Acad. Sci. USA
91:969-973, entirely incorporated by reference. In one embodiment,
the parent antibody has been affinity matured, as is known in the
art. Structure-based methods may be employed for humanization and
affinity maturation, for example as described in U.S. Ser. No.
11/004,590. Selection based methods may be employed to humanize
and/or affinity mature antibody variable regions, including but not
limited to methods described in Wu et al., 1999, J. Mol. Biol.
294:151-162; Baca et al., 1997, J. Biol. Chem. 272(16):10678-10684;
Rosok et al., 1996, J. Biol. Chem. 271(37): 22611-22618; Rader et
al., 1998, Proc. Natl. Acad. Sci. USA 95: 8910-8915; Krauss et al.,
2003, Protein Engineering 16(10):753-759, all entirely incorporated
by reference. Other humanization methods may involve the grafting
of only parts of the CDRs, including but not limited to methods
described in U.S. Ser. No. 09/810,510; Tan et al., 2002, J.
Immunol. 169:1119-1125; De Pascalis et al., 2002, J. Immunol.
169:3076-3084, all entirely incorporated by reference.
[0088] Bispecific Antibodies
[0089] In one embodiment, the antibodies of the invention
multispecific antibody, and notably a bispecific antibody, also
sometimes referred to as "diabodies". These are antibodies that
bind to two (or more) different antigens. Diabodies can be
manufactured in a variety of ways known in the art (Holliger and
Winter, 1993, Current Opinion Biotechnol. 4:446-449, entirely
incorporated by reference), e.g., prepared chemically or from
hybrid hybridomas.
[0090] Minibodies
[0091] In one embodiment, the antibody is a minibody. Minibodies
are minimized antibody-like proteins comprising a scFv joined to a
CH3 domain. Hu et al., 1996, Cancer Res. 56:3055-3061, entirely
incorporated by reference. In some cases, the scFv can be joined to
the Fc region, and may include some or the entire hinge region.
[0092] Antibody Fusions
[0093] In one embodiment, the antibodies of the invention are
antibody fusion proteins (sometimes referred to herein as an
"antibody conjugate"). One type of antibody fusions comprises Fc
fusions, which join the Fc region with a conjugate partner. By "Fc
fusion" as used herein is meant a protein wherein one or more
polypeptides is operably linked to an Fc region. Fc fusion is
herein meant to be synonymous with the terms "immunoadhesin", "Ig
fusion", "Ig chimera", and "receptor globulin" (sometimes with
dashes) as used in the prior art (Chamow et al., 1996, Trends
Biotechnol 14:52-60; Ashkenazi et al., 1997, Curr Opin Immunol
9:195-200, both entirely incorporated by reference). An Fc fusion
combines the Fc region of an immunoglobulin with a fusion partner,
which in general can be any protein or small molecule. Virtually
any protein or small molecule may be linked to Fc to generate an Fc
fusion. Protein fusion partners may include, but are not limited
to, the variable region of any antibody, the target-binding region
of a receptor, an adhesion molecule, a ligand, an enzyme, a
cytokine, a chemokine, or some other protein or protein domain.
Small molecule fusion partners may include any therapeutic agent
that directs the Fc fusion to a therapeutic target. Such targets
may be any molecule, preferably an extracellular receptor, which is
implicated in disease. Thus, the IgG variants can be linked to one
or more fusion partners. In one alternate embodiment, the IgG
variant is conjugated or operably linked to another therapeutic
compound. The therapeutic compound may be a cytotoxic agent, a
chemotherapeutic agent, a toxin, a radioisotope, a cytokine, or
other therapeutically active agent. The IgG may be linked to one of
a variety of nonproteinaceous polymers, e.g., polyethylene glycol,
polypropylene glycol, polyoxyalkylenes, or copolymers of
polyethylene glycol and polypropylene glycol.
[0094] In addition to Fc fusions, antibody fusions include the
fusion of the constant region of the heavy chain with one or more
fusion partners (again including the variable region of any
antibody), while other antibody fusions are substantially or
completely full length antibodies with fusion partners. In one
embodiment, a role of the fusion partner is to mediate target
binding, and thus it is functionally analogous to the variable
regions of an antibody (and in fact can be). Virtually any protein
or small molecule may be linked to Fc to generate an Fc fusion (or
antibody fusion). Protein fusion partners may include, but are not
limited to, the target-binding region of a receptor, an adhesion
molecule, a ligand, an enzyme, a cytokine, a chemokine, or some
other protein or protein domain. Small molecule fusion partners may
include any therapeutic agent that directs the Fc fusion to a
therapeutic target. Such targets may be any molecule, preferably an
extracellular receptor, which is implicated in disease.
[0095] The conjugate partner can be proteinaceous or
non-proteinaceous; the latter generally being generated using
functional groups on the antibody and on the conjugate partner. For
example linkers are known in the art; for example, homo-or
hetero-bifunctional linkers as are well known (see, 1994 Pierce
Chemical Company catalog, technical section on cross-linkers, pages
155-200, incorporated herein by reference).
[0096] Suitable conjugates include, but are not limited to, labels
as described below, drugs and cytotoxic agents including, but not
limited to, cytotoxic drugs (e.g., chemotherapeutic agents) or
toxins or active fragments of such toxins. Suitable toxins and
their corresponding fragments include diptheria A chain, exotoxin A
chain, ricin A chain, abrin A chain, curcin, crotin, phenomycin,
enomycin and the like. Cytotoxic agents also include radiochemicals
made by conjugating radioisotopes to antibodies, or binding of a
radionuclide to a chelating agent that has been covalently attached
to the antibody. Additional embodiments utilize calicheamicin,
auristatins, geldanamycin, maytansine, and duocarmycins and
analogs; for the latter, see U.S. 2003/0050331A1, entirely
incorporated by reference.
[0097] Covalent modifications of Antibodies
[0098] Covalent modifications of antibodies are included within the
scope of this invention, and are generally, but not always, done
post-translationally. For example, several types of covalent
modifications of the antibody are introduced into the molecule by
reacting specific amino acid residues of the antibody with an
organic derivatizing agent that is capable of reacting with
selected side chains or the N- or C-terminal residues.
[0099] Cysteinyl residues most commonly are reacted with
.alpha.-haloacetates (and corresponding amines), such as
chloroacetic acid or chloroacetamide, to give carboxymethyl or
carboxyamidomethyl derivatives. Cysteinyl residues may also be
derivatized by reaction with bromotrifluoroacetone,
.alpha.-bromo-.beta.-(5-imidozoyl)propionic acid, chloroacetyl
phosphate, N-alkylmaleimides, 3-nitro-2-pyridyl disulfide, methyl
2-pyridyl disulfide, p-chloromercuribenzoate,
2-chloromercuri-4-nitrophenol, or
chloro-7-nitrobenzo-2-oxa-1,3-diazole and the like.
[0100] Histidyl residues are derivatized by reaction with
diethylpyrocarbonate at pH 5.5-7.0 because this agent is relatively
specific for the histidyl side chain. Para-bromophenacyl bromide
also is useful; the reaction is preferably performed in 0.1M sodium
cacodylate at pH 6.0.
[0101] Lysinyl and amino terminal residues are reacted with
succinic or other carboxylic acid anhydrides. Derivatization with
these agents has the effect of reversing the charge of the lysinyl
residues. Other suitable reagents for derivatizing
alpha-amino-containing residues include imidoesters such as methyl
picolinimidate; pyridoxal phosphate; pyridoxal; chloroborohydride;
trinitrobenzenesulfonic acid; O-methylisourea; 2,4-pentanedione;
and transaminase-catalyzed reaction with glyoxylate.
[0102] Arginyl residues are modified by reaction with one or
several conventional reagents, among them phenylglyoxal,
2,3-butanedione, 1,2-cyclohexanedione, and ninhydrin.
Derivatization of arginine residues requires that the reaction be
performed in alkaline conditions because of the high pKa of the
guanidine functional group. Furthermore, these reagents may react
with the groups of lysine as well as the arginine epsilon-amino
group.
[0103] The specific modification of tyrosyl residues may be made,
with particular interest in introducing spectral labels into
tyrosyl residues by reaction with aromatic diazonium compounds or
tetranitromethane. Most commonly, N-acetylimidizole and
tetranitromethane are used to form O-acetyl tyrosyl species and
3-nitro derivatives, respectively. Tyrosyl residues are iodinated
using 125 l or 131 l to prepare labeled proteins for use in
radioimmunoassay, the chloramine T method described above being
suitable.
[0104] Carboxyl side groups (aspartyl or glutamyl) are selectively
modified by reaction with carbodiimides (R'--N.dbd.C.dbd.N--R'),
where R and R' are optionally different alkyl groups, such as
1-cyclohexyl-3-(2-morpholinyl-4-ethyl) carbodiimide or
1-ethyl-3-(4-azonia-4,4-dimethylpentyl) carbodiimide. Furthermore,
aspartyl and glutamyl residues are converted to asparaginyl and
glutaminyl residues by reaction with ammonium ions.
[0105] Derivatization with bifunctional agents is useful for
crosslinking antibodies to a water-insoluble support matrix or
surface for use in a variety of methods, in addition to methods
described below. Commonly used crosslinking agents include, e.g.,
1,1-bis(diazoacetyl)-2-phenylethane, glutaraldehyde,
N-hydroxysuccinimide esters, for example, esters with
4-azidosalicylic acid, homobifunctional imidoesters, including
disuccinimidyl esters such as 3,3'-dithiobis
(succinimidylpropionate), and bifunctional maleimides such as
bis-N-maleimido-1,8-octane. Derivatizing agents such as
methyl-3-[(p-azidophenyl)dithio]propioimidate yield
photoactivatable intermediates that are capable of forming
crosslinks in the presence of light. Alternatively, reactive
water-insoluble matrices such as cyanogen bromide-activated
carbohydrates and the reactive substrates described in U.S. Pat.
Nos. 3,969,287; 3,691,016; 4,195,128; 4,247,642; 4,229,537; and
4,330,440, all entirely incorporated by reference, are employed for
protein immobilization.
[0106] Glutaminyl and asparaginyl residues are frequently
deamidated to the corresponding glutamyl and aspartyl residues,
respectively. Alternatively, these residues are deamidated under
mildly acidic conditions. Either form of these residues falls
within the scope of this invention. [0107] Other modifications
include hydroxylation of proline and lysine, phosphorylation of
hydroxyl groups of seryl or threonyl residues, methylation of the
.alpha.-amino groups of lysine, arginine, and histidine side chains
(T. E. Creighton, Proteins: Structure and Molecular Properties, W.
H. Freeman & Co., San Francisco, pp. 79-86 [1983], entirely
incorporated by reference), acetylation of the N-terminal amine,
and amidation of any C-terminal carboxyl group.
[0108] Glycosylation
[0109] Another type of covalent modification is glycosylation. In
another embodiment, the IgG variants disclosed herein can be
modified to include one or more engineered glycoforms. By
"engineered glycoform" as used herein is meant a carbohydrate
composition that is covalently attached to an IgG, wherein said
carbohydrate composition differs chemically from that of a parent
IgG. Engineered glycoforms may be useful for a variety of purposes,
including but not limited to enhancing or reducing effector
function. Engineered glycoforms may be generated by a variety of
methods known in the art (Umana et al., 1999, Nat Biotechnol
17:176-180; Davies et al., 2001, Biotechnol Bioeng 74:288-294;
Shields et al., 2002, J Biol Chem 277:26733-26740; Shinkawa et al.,
2003, J Biol Chem 278:3466-3473; U.S. Pat. No. 6,602,684; U.S. Ser.
No. 10/277,370; U.S. Ser. No. 10/113,929; PCT WO 00/61739A1; PCT WO
01/29246A1; PCT WO 02/31140A1; PCT WO 02/30954A1, all entirely
incorporated by reference; (Potelligent.RTM. technology [Biowa,
Inc., Princeton, N.J.]; GlycoMAb.RTM. glycosylation engineering
technology [Glycart Biotechnology A G, Zurich, Switzerland]). Many
of these techniques are based on controlling the level of
fucosylated and/or bisecting oligosaccharides that are covalently
attached to the Fc region, for example by expressing an IgG in
various organisms or cell lines, engineered or otherwise (for
example Lec-13 CHO cells or rat hybridoma YB2/0 cells), by
regulating enzymes involved in the glycosylation pathway (for
example FUT8 [.alpha.1,6-fucosyltranserase] and/or
.beta.1-4-N-acetylglucosaminyltransferase III [GnTIII]), or by
modifying carbohydrate(s) after the IgG has been expressed.
Engineered glycoform typically refers to the different carbohydrate
or oligosaccharide; thus an IgG variant, for example an antibody or
Fc fusion, can include an engineered glycoform. Alternatively,
engineered glycoform may refer to the IgG variant that comprises
the different carbohydrate or oligosaccharide. As is known in the
art, glycosylation patterns can depend on both the sequence of the
protein (e.g., the presence or absence of particular glycosylation
amino acid residues, discussed below), or the host cell or organism
in which the protein is produced. Particular expression systems are
discussed below.
[0110] Glycosylation of polypeptides is typically either N-linked
or O-linked. N-linked refers to the attachment of the carbohydrate
moiety to the side chain of an asparagine residue. The tri-peptide
sequences asparagine-X-serine and asparagine-X-threonine, where X
is any amino acid except proline, are the recognition sequences for
enzymatic attachment of the carbohydrate moiety to the asparagine
side chain. Thus, the presence of either of these tri-peptide
sequences in a polypeptide creates a potential glycosylation site.
O-linked glycosylation refers to the attachment of one of the
sugars N-acetylgalactosamine, galactose, or xylose, to a
hydroxyamino acid, most commonly serine or threonine, although
5-hydroxyproline or 5-hydroxylysine may also be used.
[0111] Addition of glycosylation sites to the antibody is
conveniently accomplished by altering the amino acid sequence such
that it contains one or more of the above-described tri-peptide
sequences (for N-linked glycosylation sites). The alteration may
also be made by the addition of, or substitution by, one or more
serine or threonine residues to the starting sequence (for O-linked
glycosylation sites). For ease, the antibody amino acid sequence is
preferably altered through changes at the DNA level, particularly
by mutating the DNA encoding the target polypeptide at preselected
bases such that codons are generated that will translate into the
desired amino acids.
[0112] Another means of increasing the number of carbohydrate
moieties on the antibody is by chemical or enzymatic coupling of
glycosides to the protein. These procedures are advantageous in
that they do not require production of the protein in a host cell
that has glycosylation capabilities for N- and O-linked
glycosylation. Depending on the coupling mode used, the sugar(s)
may be attached to (a) arginine and histidine, (b) free carboxyl
groups, (c) free sulfhydryl groups such as those of cysteine, (d)
free hydroxyl groups such as those of serine, threonine, or
hydroxyproline, (e) aromatic residues such as those of
phenylalanine, tyrosine, or tryptophan, or (f) the amide group of
glutamine. These methods are described in WO 87/05330 and in Aplin
and Wriston, 1981, CRC Crit. Rev. Biochem., pp. 259-306, both
entirely incorporated by reference.
[0113] Removal of carbohydrate moieties present on the starting
antibody may be accomplished chemically or enzymatically. Chemical
deglycosylation requires exposure of the protein to the compound
trifluoromethanesulfonic acid, or an equivalent compound. This
treatment results in the cleavage of most or all sugars except the
linking sugar (N-acetylglucosamine or N-acetylgalactosamine), while
leaving the polypeptide intact. Chemical deglycosylation is
described by Hakimuddin et al., 1987, Arch. Biochem. Biophys.
259:52 and by Edge et al., 1981, Anal. Biochem. 118:131, both
entirely incorporated by reference. Enzymatic cleavage of
carbohydrate moieties on polypeptides can be achieved by the use of
a variety of endo- and exo-glycosidases as described by Thotakura
et al., 1987, Meth. Enzymol. 138:350, entirely incorporated by
reference. Glycosylation at potential glycosylation sites may be
prevented by the use of the compound tunicamycin as described by
Duskin et al., 1982, J. Biol. Chem. 257:3105, entirely incorporated
by reference. Tunicamycin blocks the formation of
protein-N-glycoside linkages.
[0114] Another type of covalent modification of the antibody
comprises linking the antibody to various nonproteinaceous
polymers, including, but not limited to, various polyols such as
polyethylene glycol, polypropylene glycol or polyoxyalkylenes, in
the manner set forth in, for example, 2005-2006 PEG Catalog from
Nektar Therapeutics (available at the Nektar website) U.S. Pat.
Nos. 4,640,835; 4,496,689; 4,301,144; 4,670,417; 4,791,192 or
4,179,337, all entirely incorporated by reference. In addition, as
is known in the art, amino acid substitutions may be made in
various positions within the antibody to facilitate the addition of
polymers such as PEG. See for example, U.S. Publication No.
2005/0114037A1, entirely incorporated by reference.
[0115] Labeled Antibodies
[0116] In some embodiments, the covalent modification of the
antibodies of the invention comprises the addition of one or more
labels. In some cases, these are considered antibody fusions. The
term "labelling group" means any detectable label. In some
embodiments, the labelling group is coupled to the antibody via
spacer arms of various lengths to reduce potential steric
hindrance. Various methods for labelling proteins are known in the
art and may be used in performing the present invention.
[0117] In general, labels fall into a variety of classes, depending
on the assay in which they are to be detected: a) isotopic labels,
which may be radioactive or heavy isotopes; b) magnetic labels
(e.g., magnetic particles); c) redox active moieties; d) optical
dyes; enzymatic groups (e.g. horseradish peroxidase,
.beta.-galactosidase, luciferase, alkaline phosphatase); e)
biotinylated groups; and f) predetermined polypeptide epitopes
recognized by a secondary reporter (e.g., leucine zipper pair
sequences, binding sites for secondary antibodies, metal binding
domains, epitope tags, etc.). In some embodiments, the labelling
group is coupled to the antibody via spacer arms of various lengths
to reduce potential steric hindrance. Various methods for labelling
proteins are known in the art and may be used in performing the
present invention.
[0118] Specific labels include optical dyes, including, but not
limited to, chromophores, phosphors and fluorophores, with the
latter being specific in many instances. Fluorophores can be either
"small molecule" fluores, or proteinaceous fluores.
[0119] By "fluorescent label" is meant any molecule that may be
detected via its inherent fluorescent properties. Suitable
fluorescent labels include, but are not limited to, fluorescein,
rhodamine, tetramethylrhodamine, eosin, erythrosin, coumarin,
methyl-coumarins, pyrene, Malacite green, stilbene, Lucifer Yellow,
Cascade BlueJ, Texas Red, IAEDANS, EDANS, BODIPY FL, LC Red 640, Cy
5, Cy 5.5, LC Red 705, Oregon green, the Alexa-Fluor dyes (Alexa
Fluor 350, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa
Fluor 568, Alexa Fluor 594, Alexa Fluor 633, Alexa Fluor 660, Alexa
Fluor 680), Cascade Blue, Cascade Yellow and R-phycoerythrin (PE)
(Molecular Probes, Eugene, Oreg.), FITC, Rhodamine, and Texas Red
(Pierce, Rockford, Ill.), Cy5, Cy5.5, Cy7 (Amersham Life Science,
Pittsburgh, Pa.). Suitable optical dyes, including fluorophores,
are described in Molecular Probes Handbook by Richard P. Haugland,
entirely incorporated by reference.
[0120] Suitable proteinaceous fluorescent labels also include, but
are not limited to, green fluorescent protein, including a Renilla,
Ptilosarcus, or Aequorea species of GFP (Chalfie et al., 1994,
Science 263:802-805), EGFP (Clontech Laboratories, Inc., Genbank
Accession Number U55762), blue fluorescent protein (BFP, Quantum
Biotechnologies, Inc. 1801 de Maisonneuve Blvd. West, 8th Floor,
Montreal, Quebec, Canada H3H 1J9; Stauber, 1998, Biotechniques
24:462-471; Heim et al., 1996, Curr. Biol. 6:178-182), enhanced
yellow fluorescent protein (EYFP, Clontech Laboratories, Inc.),
luciferase (Ichiki et al., 1993, J. Immunol. 150:5408-5417), .beta.
galactosidase (Nolan et al., 1988, Proc. Natl. Acad. Sci. U.S.A.
85:2603-2607) and Renilla (WO92/15673, WO95/07463, WO98/14605,
WO98/26277, WO99/49019, U.S. Pat. Nos. 5,292,658, 5,418,155,
5,683,888, 5,741,668, 5,777,079, 5,804,387, 5,874,304, 5,876,995,
5,925,558). All of the above-cited references in this paragraph are
expressly incorporated herein by reference.
[0121] IgG Variants
[0122] In one embodiment, the invention provides variant IgG
proteins. At a minimum, IgG variants comprise an antibody fragment
comprising the CH2--CH3 region of the heavy chain. In addition,
suitable IgG variants comprise Fc domains (e.g. including the lower
hinge region), as well as IgG variants comprising the constant
region of the heavy chain (CH1-hinge-CH2--CH3) also being useful in
the present invention, all of which can be fused to fusion
partners.
[0123] An IgG variant includes one or more amino acid modifications
relative to a parent IgG polypeptide, in some cases relative to the
wild type IgG. The IgG variant can have one or more optimized
properties. An IgG variant differs in amino acid sequence from its
parent IgG by virtue of at least one amino acid modification. Thus
IgG variants have at least one amino acid modification compared to
the parent. Alternatively, the IgG variants may have more than one
amino acid modification as compared to the parent, for example from
about one to fifty amino acid modifications, preferably from about
one to ten amino acid modifications, and most preferably from about
one to about five amino acid modifications compared to the
parent.
[0124] Thus the sequences of the IgG variants and those of the
parent Fc polypeptide are substantially homologous. For example,
the variant IgG variant sequences herein will possess about 80%
homology with the parent IgG variant sequence, preferably at least
about 90% homology, and most preferably at least about 95%
homology. Modifications may be made genetically using molecular
biology, or may be made enzymatically or chemically.
[0125] Target Antigens for Antibodies
[0126] Virtually any antigen may be targeted by the IgG variants,
including but not limited to proteins, subunits, domains, motifs,
and/or epitopes belonging to the following list of target antigens,
which includes both soluble factors such as cytokines and
membrane-bound factors, including transmembrane receptors: 17-IA,
4-1BB, 4Dc, 6-keto-PGF1a, 8-iso-PGF2a, 8-oxo-dG, A1 Adenosine
Receptor, A33, ACE, ACE-2, Activin, Activin A, Activin AB, Activin
B, Activin C, Activin RIA, Activin RIA ALK-2, Activin RIB ALK-4,
Activin RIIA, Activin RIIB, ADAM, ADAM10, ADAM12, ADAM15,
ADAM17/TACE, ADAM8, ADAM9, ADAMTS, ADAMTS4, ADAMTS5, Addressins,
aFGF, ALCAM, ALK, ALK-1, ALK-7, alpha-1-antitrypsin, alpha-V/beta-1
antagonist, ANG, Ang, APAF-1, APE, APJ, APP, APRIL, AR, ARC, ART,
Artemin, anti-Id, ASPARTIC, Atrial natriuretic factor, av/b3
integrin, Axl, b2M, B7-1, B7-2, B7-H, B-lymphocyte Stimulator
(BlyS), BACE, BACE-1, Bad, BAFF, BAFF-R, Bag-1, BAK, Bax, BCA-1,
BCAM, Bcl, BCMA, BDNF, b-ECGF, bFGF, BID, Bik, BIM, BLC, BL-CAM,
BLK, BMP, BMP-2 BMP-2a, BMP-3 Osteogenin, BMP-4 BMP-2b, BMP-5,
BMP-6 Vgr-1, BMP-7 (OP-1), BMP-8 (BMP-8a, OP-2), BMPR, BMPR-IA
(ALK-3), BMPR-IB (ALK-6), BRK-2, RPK-1, BMPR-II (BRK-3), BMPs,
b-NGF, BOK, Bombesin, Bone-derived neurotrophic factor, BPDE,
BPDE-DNA, BTC, complement factor 3 (C3), C3a, C4, C5, C5a, C10,
CA125, CAD-8, Calcitonin, cAMP, carcinoembryonic antigen (CEA),
carcinoma-associated antigen, Cathepsin A, Cathepsin B, Cathepsin
C/DPPI, Cathepsin D, Cathepsin E, Cathepsin H, Cathepsin L,
Cathepsin O, Cathepsin S, Cathepsin V, Cathepsin X/Z/P, CBL, CCI,
CCK2, CCL, CCL1, CCL11, CCL12, CCL13, CCL14, CCL15, CCL16, CCL17,
CCL18, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25,
CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8, CCL9/10,
CCR, CCR1, CCR10, CCR10, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8,
CCR9, CD1, CD2, CD3, CD3E, CD4, CD5, CD6, CD7, CD8, CD10, CD11a,
CD11b, CD11c, CD13, CD14, CD15, CD16, CD18, CD19, CD20, CD21, CD22,
CD23, CD25, CD27L, CD28, CD29, CD30, CD30L, CD32, CD33 (p67
proteins), CD34, CD38, CD40, CD40L, CD44, CD45, CD46, CD49a, CD52,
CD54, CD55, CD56, CD61, CD64, CD66e, CD74, CD80 (B7-1), CD89, CD95,
CD123, CD137, CD138, CD140a, CD146, CD147, CD148, CD152, CD164,
CEACAM5, CFTR, cGMP, ClNC, Clostridium botulinum toxin, Clostridium
perfringens toxin, CKb8-1, CLC, CMV, CMV UL, CNTF, CNTN-1, COX,
C-Ret, CRG-2, CT-1, CTACK, CTGF, CTLA-4, CX3CL1, CX3CR1, CXCL,
CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9,
CXCL10, CXCL11, CXCL12, CXCL13, CXCL14, CXCL15, CXCL16, CXCR,
CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, cytokeratin
tumor-associated antigen, DAN, DCC, DcR3, DC-SIGN, Decay
accelerating factor, des(1-3)-IGF-I (brain IGF-1), Dhh, digoxin,
DNAM-1, Dnase, Dpp, DPPIV/CD26, Dtk, ECAD, EDA, EDA-A1, EDA-A2,
EDAR, EGF, EGFR (ErbB-1), EMA, EMMPRIN, ENA, endothelin receptor,
Enkephalinase, eNOS, Eot, eotaxin1, EpCAM, Ephrin B2/EphB4, EPO,
ERCC, E-selectin, ET-1, Factor IIa, Factor VII, Factor VIIIc,
Factor IX, fibroblast activation protein (FAP), Fas, FcR1, FEN-1,
Ferritin, FGF, FGF-19, FGF-2, FGF3, FGF-8, FGFR, FGFR-3, Fibrin,
FL, FLIP, Flt-3, Flt-4, Follicle stimulating hormone, Fractalkine,
FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD9, FZD10, G250,
Gas 6, GCP-2, GCSF, GD2, GD3, GDF, GDF-1, GDF-3 (Vgr-2), GDF-5
(BMP-14, CDMP-1), GDF-6 (BMP-13, CDMP-2), GDF-7 (BMP-12, CDMP-3),
GDF-8 (Myostatin), GDF-9, GDF-15 (MIC-1), GDNF, GDNF, GFAP, GFRa-1,
GFR-alpha1, GFR-alpha2, GFR-alpha3, GITR, Glucagon, Glut 4,
glycoprotein IIb/IIIa (GP IIb/IIIa), GM-CSF, gp130, gp72, GRO,
Growth hormone releasing factor, Hapten (NP-cap or NIP-cap),
HB-EGF, HCC, HCMV gB envelope glycoprotein, HCMV) gH envelope
glycoprotein, HCMV UL, Hemopoietic growth factor (HGF), Hep B
gp120, heparanase, Her2, Her2/neu (ErbB-2), Her3 (ErbB-3), Her4
(ErbB-4), herpes simplex virus (HSV) gB glycoprotein, HSV gD
glycoprotein, HGFA, High molecular weight melanoma-associated
antigen (HMW-MAA), HIV gp120, HIV IIIB gp 120 V3 loop, HLA, HLA-DR,
HM1.24, HMFG PEM, HRG, Hrk, human cardiac myosin, human
cytomegalovirus (HCMV), human growth hormone (HGH), HVEM, I-309,
IAP, ICAM, ICAM-1, ICAM-3, ICE, ICOS, IFNg, Ig, IgA receptor, IgE,
IGF, IGF binding proteins, IGF-1R, IGFBP, IGF-I, IGF-II, IL, IL-1,
IL-1R, IL-2, IL-2R, IL-4, IL-4R, IL-5, IL-5R, IL-6, IL-6R, IL-8,
IL-9, IL-10, IL-12, IL-13, IL-15, IL-18, IL-18R, IL-23, interferon
(INF)-alpha, INF-beta, INF-gamma, Inhibin, iNOS, Insulin A-chain,
Insulin B-chain, Insulin-like growth factor 1, integrin alpha2,
integrin alpha3, integrin alpha4, integrin alpha4/beta1, integrin
alpha4/beta7, integrin alpha5 (alphaV), integrin alpha5/beta1,
integrin alpha5/beta3, integrin alpha6, integrin beta1, integrin
beta2, interferon gamma, IP-10, I-TAC, JE, Kallikrein 2, Kallikrein
5, Kallikrein 6, Kallikrein 11, Kallikrein 12, Kallikrein 14,
Kallikrein 15, Kallikrein L1, Kallikrein L2, Kallikrein L3,
Kallikrein L4, KC, KDR, Keratinocyte Growth Factor (KGF), laminin
5, LAMP, LAP, LAP (TGF-1), Latent TGF-1, Latent TGF-1 bpi, LBP,
LDGF, LECT2, Lefty, Lewis-Y antigen, Lewis-Y related antigen,
LFA-1, LFA-3, Lfo, LIF, LIGHT, lipoproteins, LIX, LKN, Lptn,
L-Selectin, LT-a, LT-b, LTB4, LTBP-1, Lung surfactant, Luteinizing
hormone, Lymphotoxin Beta Receptor, Mac-1, MAdCAM, MAG, MAP2, MARC,
MCAM, MCAM, MCK-2, MCP, M-CSF, MDC, Mer, METALLOPROTEASES, MGDF
receptor, MGMT, MHC (HLA-DR), MIF, MIG, MIP, MIP-1-alpha, MK,
MMAC1, MMP, MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15,
MMP-2, MMP-24, MMP-3, MMP-7, MMP-8, MMP-9, MPIF, Mpo, MSK, MSP,
mucin (Muc1), MUC18, Muellerian-inhibitin substance, Mug, MuSK,
NAIP, NAP, NCAD, N-Cadherin, NCA 90, NCAM, NCAM, Neprilysin,
Neurotrophin-3,-4, or -6, Neurturin, Neuronal growth factor (NGF),
NGFR, NGF-beta, nNOS, NO, NOS, Npn, NRG-3, NT, NTN, OB, OGG1, OPG,
OPN, OSM, OX40L, OX40R, p150, p95, PADPr, Parathyroid hormone,
PARC, PARP, PBR, PBSF, PCAD, P-Cadherin, PCNA, PDGF, PDGF, PDK-1,
PECAM, PEM, PF4, PGE, PGF, PGI2, PGJ2, PIN, PLA2, placental
alkaline phosphatase (PLAP), PIGF, PLP, PP14, Proinsulin,
Prorelaxin, Protein C, PS, PSA, PSCA, prostate specific membrane
antigen (PSMA), PTEN, PTHrp, Ptk, PTN, R51, RANK, RANKL, RANTES,
RANTES, Relaxin A-chain, Relaxin B-chain, renin, respiratory
syncytial virus (RSV) F, RSV Fgp, Ret, Rheumatoid factors, RLIP76,
RPA2, RSK, S100, SCF/KL, SDF-1, SERINE, Serum albumin, sFRP-3, Shh,
SIGIRR, SK-1, SLAM, SLPI, SMAC, SMDF, SMOH, SOD, SPARC, Stat,
STEAP, STEAP-II, TACE, TACI, TAG-72 (tumor-associated
glycoprotein-72), TARC, TCA-3, T-cell receptors (e.g., T-cell
receptor alpha/beta), TdT, TECK, TEM1, TEM5, TEM7, TEM8, TERT,
testicular PLAP-like alkaline phosphatase, TfR, TGF, TGF-alpha,
TGF-beta, TGF-beta Pan Specific, TGF-beta RI (ALK-5), TGF-beta RII,
TGF-beta RIIb, TGF-beta RIII, TGF-beta1, TGF-beta2, TGF-beta3,
TGF-beta4, TGF-beta5, Thrombin, Thymus Ck-1, Thyroid stimulating
hormone, Tie, TIMP, TIQ, Tissue Factor, TMEFF2, Tmpo, TMPRSS2, TNF,
TNF-alpha, TNF-alpha beta, TNF-beta2, TNFc, TNF-RI, TNF-RII,
TNFRSF10A (TRAIL R1 Apo-2, DR4), TNFRSF10B (TRAIL R2 DR5, KILLER,
TRICK-2A, TRICK-B), TNFRSF10C (TRAIL R3 DcR1, LIT, TRID), TNFRSF10D
(TRAIL R4 DcR2, TRUNDD), TNFRSF11A (RANK ODF R, TRANCE R),
TNFRSF11B (OPG OCIF, TR1), TNFRSF12 (TWEAK R FN14), TNFRSF13B
(TACI), TNFRSF13C (BAFF R), TNFRSF14 (HVEM ATAR, HveA, LIGHT R,
TR2), TNFRSF16 (NGFR p75NTR), TNFRSF17 (BCMA), TNFRSF18 (GITR
AITR), TNFRSF19 (TROY TAJ, TRADE), TNFRSF19L (RELT), TNFRSF1A (TNF
RI CD120a, p55-60), TNFRSF1B (TNF RII CD120b, p75-80), TNFRSF26
(TNFRH3), TNFRSF3 (LTbR TNF RIII, TNFC R), TNFRSF4 (OX40 ACT35,
TXGP1 R), TNFRSF5 (CD40 p50), TNFRSF6 (Fas Apo-1, APT1, CD95),
TNFRSF6B (DcR3 M68, TR6), TNFRSF7 (CD27), TNFRSF8 (CD30), TNFRSF9
(4-1BB CD137, ILA), TNFRSF21 (DR6), TNFRSF22 (DcTRAIL R2 TNFRH2),
TNFRST23 (DcTRAIL R1 TNFRH1), TNFRSF25 (DR3 Apo-3, LARD, TR-3,
TRAMP, WSL-1), TNFSF10 (TRAIL Apo-2 Ligand, TL2), TNFSF11
(TRANCE/RANK Ligand ODF, OPG Ligand), TNFSF12 (TWEAK Apo-3 Ligand,
DR3 Ligand), TNFSF13 (APRIL TALL2), TNFSF13B (BAFF BLYS, TALL1,
THANK, TNFSF20), TNFSF14 (LIGHT HVEM Ligand, LTg), TNFSF15
(TL1A/VEGI), TNFSF18 (GITR Ligand AITR Ligand, TL6), TNFSF1A (TNF-a
Conectin, DIF, TNFSF2), TNFSF1B (TNF-b LTa, TNFSF1), TNFSF3 (LTb
TNFC, p33), TNFSF4 (OX40 Ligand gp34, TXGP1), TNFSF5 (CD40 Ligand
CD154, gp39, HIGM1, IMD3, TRAP), TNFSF6 (Fas Ligand Apo-1 Ligand,
APT1 Ligand), TNFSF7 (CD27 Ligand CD70), TNFSF8 (CD30 Ligand
CD153), TNFSF9 (4-1BB Ligand CD137 Ligand), TP-1, t-PA, Tpo, TRAIL,
TRAIL R, TRAIL-R1, TRAIL-R2, TRANCE, transferring receptor, TRF,
Trk, TROP-2, TSG, TSLP, tumor-associated antigen CA 125,
tumor-associated antigen expressing Lewis Y related carbohydrate,
TWEAK, TXB2, Ung, uPAR, uPAR-1, Urokinase, VCAM, VCAM-1, VECAD,
VE-Cadherin, VE-cadherin-2, VEFGR-1 (flt-1), VEGF, VEGFR, VEGFR-3
(flt-4), VEGI, VIM, Viral antigens, VLA, VLA-1, VLA-4, VNR
integrin, von Willebrands factor, WIF-1, WNT1, WNT2, WNT2B/13,
WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A, WNT7B, WNT8A, WNT8B,
WNT9A, WNT9A, WNT9B, WNT10A, WNT10B, WNT11, WNT16, XCL1, XCL2,
XCR1, XCR1, XEDAR, XIAP, XPD, and receptors for hormones and growth
factors.
[0127] One skilled in the art will appreciate that the
aforementioned list of targets refers not only to specific proteins
and biomolecules, but the biochemical pathway or pathways that
comprise them. For example, reference to CTLA-4 as a target antigen
implies that the ligands and receptors that make up the T cell
co-stimulatory pathway, including CTLA-4, B7-1, B7-2, CD28, and any
other undiscovered ligands or receptors that bind these proteins,
are also targets. Thus target as used herein refers not only to a
specific biomolecule, but the set of proteins that interact with
said target and the members of the biochemical pathway to which
said target belongs. One skilled in the art will further appreciate
that any of the aforementioned target antigens, the ligands or
receptors that bind them, or other members of their corresponding
biochemical pathway, may be operably linked to the Fc variants of
the present invention in order to generate an Fc fusion. Thus for
example, an Fc fusion that targets EGFR could be constructed by
operably linking an Fc variant to EGF, TGF-b, or any other ligand,
discovered or undiscovered, that binds EGFR. Accordingly, an Fc
variant of the present invention could be operably linked to EGFR
in order to generate an Fc fusion that binds EGF, TGF-b, or any
other ligand, discovered or undiscovered, that binds EGFR. Thus
virtually any polypeptide, whether a ligand, receptor, or some
other protein or protein domain, including but not limited to the
aforementioned targets and the proteins that compose their
corresponding biochemical pathways, may be operably linked to the
Fc variants of the present invention to develop an Fc fusion.
[0128] The choice of suitable antigen depends on the desired
application. For anti-cancer treatment it is desirable to have a
target whose expression is restricted to the cancerous cells. Some
targets that have proven especially amenable to antibody therapy
are those with signaling functions. Other therapeutic antibodies
exert their effects by blocking signaling of the receptor by
inhibiting the binding between a receptor and its cognate ligand.
Another mechanism of action of therapeutic antibodies is to cause
receptor down regulation. Other antibodies do not work by signaling
through their target antigen. In some cases, antibodies directed
against infectious disease agents are used.
[0129] In one embodiment, the Fc variants of the present invention
are incorporated into an antibody against a cytokine.
Alternatively, the Fc variants are fused or conjugated to a
cytokine. By "cytokine" as used herein is meant a generic term for
proteins released by one cell population that act on another cell
as intercellular mediators. For example, as described in Penichet
et al., 2001, J Immunol Methods 248:91-101, expressly incorporated
by reference, cytokines may be fused to antibody to provide an
array of desirable properties. Examples of such cytokines are
lymphokines, monokines, and traditional polypeptide hormones.
Included among the cytokines are growth hormone such as human
growth hormone, N-methionyl human growth hormone, and bovine growth
hormone; parathyroid hormone; thyroxine; insulin; proinsulin;
relaxin; prorelaxin; glycoprotein hormones such as follicle
stimulating hormone (FSH), thyroid stimulating hormone (TSH), and
luteinizing hormone (LH); hepatic growth factor; fibroblast growth
factor; prolactin; placental lactogen; tumor necrosis factor-alpha
and -beta; mullerian-inhibiting substance; mouse
gonadotropin-associated peptide; inhibin; activin; vascular
endothelial growth factor; integrin; thrombopoietin (TPO); nerve
growth factors such as NGF-beta; platelet-growth factor;
transforming growth factors (TGFs) such as TGF-alpha and TGF-beta;
insulin-like growth factor-I and -II; erythropoietin (EPO);
osteoinductive factors; interferons such as interferon-alpha, beta,
and -gamma; colony stimulating factors (CSFs) such as
macrophage-CSF (M-CSF); granulocyte-macrophage-CSF (GM-CSF); and
granulocyte-CSF (G-CSF); interleukins (ILs) such as IL-1,
IL-1alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10,
IL-11, IL-12; IL-15, a tumor necrosis factor such as TNF-alpha or
TNF-beta; C5a; and other polypeptide factors including LIF and kit
ligand (KL). As used herein, the term cytokine includes proteins
from natural sources or from recombinant cell culture, and
biologically active equivalents of the native sequence
cytokines.
[0130] Cytokines and soluble targets, such as TNF superfamily
members, are preferred targets for use with the variants of the
present invention. For example, anti-VEGF, anti-CTLA-4, and
anti-TNF antibodies, or fragments thereof, are particularly good
antibodies for the use of Fc variants that increase the FcRn
binding. Therapeutics against these targets are frequently involved
in the treatment of autoimmune diseases and require multiple
injections over long time periods. Therefore, longer serum
half-lives and less frequent treatments, brought about from the
variants of the present invention, are particularly preferred.
[0131] A number of antibodies and Fc fusions that are approved for
use, in clinical trials, or in development may benefit from the Fc
variants of the present invention. These antibodies and Fc fusions
are herein referred to as "clinical products and candidates". Thus
in a preferred embodiment, the Fc polypeptides of the present
invention may find use in a range of clinical products and
candidates. For example, a number of antibodies that target CD20
may benefit from the Fc polypeptides of the present invention. For
example the Fc polypeptides of the present invention may find use
in an antibody that is substantially similar to rituximab
(Rituxan.RTM., IDEC/Genentech/Roche) (see for example U.S. Pat. No.
5,736,137), a chimeric anti-CD20 antibody approved to treat
Non-Hodgkin's lymphoma; HuMax-CD20, an anti-CD20 currently being
developed by Genmab, an anti-CD20 antibody described in U.S. Pat.
No. 5,500,362, AME-133 (Applied Molecular Evolution), hA20
(Immunomedics, Inc.), HumaLYM (Intracel), and PRO70769
(PCT/US2003/040426, entitled "Immunoglobulin Variants and Uses
Thereof"). A number of antibodies that target members of the family
of epidermal growth factor receptors, including EGFR (ErbB-1),
Her2/neu (ErbB-2), Her3 (ErbB-3), Her4 (ErbB-4), may benefit from
the Fc polypeptides of the present invention. For example the Fc
polypeptides of the present invention may find use in an antibody
that is substantially similar to trastuzumab (Herceptin.RTM.,
Genentech) (see for example U.S. Pat. No. 5,677,171), a humanized
anti-Her2/neu antibody approved to treat breast cancer; pertuzumab
(rhuMab-2C4, Omnitarg.TM.), currently being developed by Genentech;
an anti-Her2 antibody described in U.S. Pat. No. 4,753,894;
cetuximab (Erbitux.RTM., Imclone) (U.S. Pat. No. 4,943,533; PCT WO
96/40210), a chimeric anti-EGFR antibody in clinical trials for a
variety of cancers; ABX-EGF (U.S. Pat. No. 6,235,883), currently
being developed by Abgenix-Immunex-Amgen; HuMax-EGFr (U.S. Ser. No.
10/172,317), currently being developed by Genmab; 425, EMD55900,
EMD62000, and EMD72000 (Merck KGaA) (U.S. Pat. No. 5,558,864;
Murthy et al. 1987, Arch Biochem Biophys. 252(2):549-60; Rodeck et
al., 1987, J Cell Biochem. 35(4):315-20; Kettleborough et al.,
1991, Protein Eng. 4(7):773-83); ICR62 (Institute of Cancer
Research) (PCT WO 95/20045; Modjtahedi et al., 1993, J. Cell
Biophys. 1993, 22(1-3):129-46; Modjtahedi et al., 1993, Br J
Cancer. 1993, 67(2):247-53; Modjtahedi et al, 1996, Br J Cancer,
73(2):228-35; Modjtahedi et al, 2003, Int J Cancer, 105(2):273-80);
TheraCIM hR3 (YM Biosciences, Canada and Centro de Immunologia
Molecular, Cuba (U.S. Pat. No. 5,891,996; U.S. Pat. No. 6, 506,883;
Mateo et al, 1997, Immunotechnology, 3(1):71-81); mAb-806 (Ludwig
Institue for Cancer Research, Memorial Sloan-Kettering) (Jungbluth
et al. 2003, Proc Natl Acad Sci USA. 100(2):639-44); KSB-102 (KS
Biomedix); MR1-1 (IVAX, National Cancer Institute) (PCT WO
0162931A2); and SC100 (Scancell) (PCT WO 01/88138). In another
preferred embodiment, the Fc polypeptides of the present invention
may find use in alemtuzumab (Campath.RTM., Millenium), a humanized
monoclonal antibody currently approved for treatment of B-cell
chronic lymphocytic leukemia. The Fc polypeptides of the present
invention may find use in a variety of antibodies or Fc fusions
that are substantially similar to other clinical products and
candidates, including but not limited to muromonab-CD3 (Orthoclone
OKT3.RTM.), an anti-CD3 antibody developed by Ortho Biotech/Johnson
& Johnson, ibritumomab tiuxetan (Zevalin.RTM.), an anti-CD20
antibody developed by IDEC/Schering A G, gemtuzumab ozogamicin
(Mylotarg.RTM.), an anti-CD33 (p67 protein) antibody developed by
Celltech/Wyeth, alefacept (Amevive.RTM.), an anti-LFA-3 Fc fusion
developed by Biogen), abciximab (ReoPro.RTM.), developed by
Centocor/Lilly, basiliximab (Simulect.RTM.), developed by Novartis,
palivizumab (Synagis.RTM.), developed by MedImmune, infliximab
(Remicade.RTM.), an anti-TNFalpha antibody developed by Centocor,
adalimumab (Humira.RTM.), an anti-TNFalpha antibody developed by
Abbott, Humicade.TM., an anti-TNFalpha antibody developed by
Celltech, etanercept (Enbrel.RTM.), an anti-TNFalpha Fc fusion
developed by Immunex/Amgen, ABX-CBL, an anti-CD147 antibody being
developed by Abgenix, ABX-IL8, an anti-IL8 antibody being developed
by Abgenix, ABX-MA1, an anti-MUC18 antibody being developed by
Abgenix, Pemtumomab (R1549, 90Y-muHMFG1), an anti-MUC1 In
development by Antisoma, Therex (R1550), an anti-MUC1 antibody
being developed by Antisoma, AngioMab (AS1405), being developed by
Antisoma, HuBC-1, being developed by Antisoma, Thioplatin (AS1407)
being developed by Antisoma, Antegren.RTM. (natalizumab), an
anti-alpha-4-beta-1 (VLA-4) and alpha-4-beta-7 antibody being
developed by Biogen, VLA-1 mAb, an anti-VLA-1 integrin antibody
being developed by Biogen, LTBR mAb, an anti-lymphotoxin beta
receptor (LTBR) antibody being developed by Biogen, CAT-152, an
anti-TGF-.beta.2 antibody being developed by Cambridge Antibody
Technology, J695, an anti-IL-12 antibody being developed by
Cambridge Antibody Technology and Abbott, CAT-192, an
anti-TGF.beta.1 antibody being developed by Cambridge Antibody
Technology and Genzyme, CAT-213, an anti-Eotaxin1 antibody being
developed by Cambridge Antibody Technology, LymphoStat-B.TM. an
anti-Blys antibody being developed by Cambridge Antibody Technology
and Human Genome Sciences Inc., TRAIL-R1 mAb, an anti-TRAIL-R1
antibody being developed by Cambridge Antibody Technology and Human
Genome Sciences, Inc., Avastin.TM. (bevacizumab, rhuMAb-VEGF), an
anti-VEGF antibody being developed by Genentech, an anti-HER
receptor family antibody being developed by Genentech, Anti-Tissue
Factor (ATF), an anti-Tissue Factor antibody being developed by
Genentech, Xolair.TM. (Omalizumab), an anti-IgE antibody being
developed by Genentech, Raptiva.TM. (Efalizumab), an anti-CD11a
antibody being developed by Genentech and Xoma, MLN-02 Antibody
(formerly LDP-02), being developed by Genentech and Millenium
Pharmaceuticals, HuMax CD4, an anti-CD4 antibody being developed by
Genmab, HuMax-IL15, an anti-IL15 antibody being developed by Genmab
and Amgen, HuMax-Inflam, being developed by Genmab and Medarex,
HuMax-Cancer, an anti-Heparanase I antibody being developed by
Genmab and Medarex and Oxford GcoSciences, HuMax-Lymphoma, being
developed by Genmab and Amgen, HuMax-TAC, being developed by
Genmab, IDEC-131, and anti-CD40L antibody being developed by IDEC
Pharmaceuticals, IDEC-151 (Clenoliximab), an anti-CD4 antibody
being developed by IDEC Pharmaceuticals, IDEC-114, an anti-CD80
antibody being developed by IDEC Pharmaceuticals, IDEC-152, an
anti-CD23 being developed by IDEC Pharmaceuticals, anti-macrophage
migration factor (MIF) antibodies being developed by IDEC
Pharmaceuticals, BEC2, an anti-idiotypic antibody being developed
by Imclone, IMC-1C11, an anti-KDR antibody being developed by
Imclone, DC101, an anti-flk-1 antibody being developed by Imclone,
anti-VE cadherin antibodies being developed by Imclone,
CEA-Cide.TM. (labetuzumab), an anti-carcinoembryonic antigen (CEA)
antibody being developed by Immunomedics, LymphoCide.TM.
(Epratuzumab), an anti-CD22 antibody being developed by
Immunomedics, AFP-Cide, being developed by Immunomedics,
MyelomaCide, being developed by Immunomedics, LkoCide, being
developed by Immunomedics, ProstaCide, being developed by
Immunomedics, MDX-010, an anti-CTLA4 antibody being developed by
Medarex, MDX-060, an anti-CD30 antibody being developed by Medarex,
MDX-070 being developed by Medarex, MDX-018 being developed by
Medarex, Osidem.TM. (IDM-1), and anti-Her2 antibody being developed
by Medarex and Immuno-Designed Molecules, HuMax.TM.-CD4, an
anti-CD4 antibody being developed by Medarex and Genmab,
HuMax-IL15, an anti-IL15 antibody being developed by Medarex and
Genmab, CNTO 148, an anti-TNF.alpha. antibody being developed by
Medarex and Centocor/J&J, CNTO 1275, an anti-cytokine antibody
being developed by Centocor/J&J, MOR101 and MOR102,
anti-intercellular adhesion molecule-1 (ICAM-1) (CD54) antibodies
being developed by MorphoSys, MOR201, an anti-fibroblast growth
factor receptor 3 (FGFR-3) antibody being developed by MorphoSys,
Nuvion.RTM. (visilizumab), an anti-CD3 antibody being developed by
Protein Design Labs, HuZAF.TM., an anti-gamma interferon antibody
being developed by Protein Design Labs, Anti-.alpha.5.beta.1
Integrin, being developed by Protein Design Labs, anti-IL-12, being
developed by Protein Design Labs, ING-1, an anti-Ep-CAM antibody
being developed by Xoma, and MLN01, an anti-Beta2 integrin antibody
being developed by Xoma, all of the above-cited references in this
paragraph are expressly incorporated herein by reference.
[0132] The Fc polypeptides of the present invention may be
incorporated into the aforementioned clinical candidates and
products, or into antibodies and Fc fusions that are substantially
similar to them. The Fc polypeptides of the present invention may
be incorporated into versions of the aforementioned clinical
candidates and products that are humanized, affinity matured,
engineered, or modified in some other way.
[0133] In one embodiment, the Fc polypeptides of the present
invention are used for the treatment of autoimmune, inflammatory,
or transplant indications. Target antigens and clinical products
and candidates that are relevant for such diseases include but are
not limited to anti-.alpha.4.beta.7 integrin antibodies such as
LDP-02, anti-beta2 integrin antibodies such as LDP-01,
anti-complement (C5) antibodies such as 5G1.1, anti-CD2 antibodies
such as BTI-322, MEDI-507, anti-CD3 antibodies such as OKT3, SMART
anti-CD3, anti-CD4 antibodies such as IDEC-151, MDX-CD4, OKT4A,
anti-CD11a antibodies, anti-CD14 antibodies such as IC14, anti-CD18
antibodies, anti-CD23 antibodies such as IDEC 152, anti-CD25
antibodies such as Zenapax, anti-CD40L antibodies such as 5c8,
Antova, IDEC-131, anti-CD64 antibodies such as MDX-33, anti-CD80
antibodies such as IDEC-114, anti-CD147 antibodies such as ABX-CBL,
anti-E-selectin antibodies such as CDP850, anti-gpIIb/IIIa
antibodies such as ReoPro/Abcixima, anti-ICAM-3 antibodies such as
ICM3, anti-ICE antibodies such as VX-740, anti-FcR1 antibodies such
as MDX-33, anti-IgE antibodies such as rhuMab-E25, anti-IL-4
antibodies such as SB-240683, anti-IL-5 antibodies such as
SB-240563, SCH55700, anti-IL-8 antibodies such as ABX-IL8,
anti-interferon gamma antibodies, anti-TNF (TNF, TNFa, TNFa,
TNF-alpha) antibodies such as CDP571, CDP870, D2E7, Infliximab,
MAK-195F, and anti-VLA-4 antibodies such as Antegren.
[0134] Fc variants of the present invention such as those with
increased binding to FcRn may be utilized in TNF inhibitor
molecules to provide enhanced properties. Useful TNF inhibitor
molecules include any molecule that inhibits the action of
TNF-alpha in a mammal. Suitable examples include the Fc fusion
Enbrel.RTM. (etanercept) and the antibodies Humira.RTM.
(adalimumab) and Remicade.RTM. (infliximab). Monoclonal antibodies
(such as Remicade and Humira) engineered using the Fc variants of
the present invention to increase FcFn binding, may translate to
better efficacy through an increased half-life.
[0135] In some embodiments, antibodies against infectious diseases
are used. Antibodies against eukaryotic cells include antibodies
targeting yeast cells, including but not limited to Saccharomyces
cerevisiae, Hansenula polymorpha, Kluyveromyces fragilis and K.
lactis, Pichia guillerimondii and P. pastoris, Schizosaccharomyces
pombe, plasmodium falciparium, and Yarrowia lipolytica.
[0136] Antibodies against additional fungal cells are also useful,
including target antigens associated with Candida strains including
Candida glabrata, Candida albicans, C. krusei, C. lusitaniaeand C.
maltosa, as well as species of Aspergillus, Cryptococcus,
Histoplasma, Coccidioides, Blastomyces, and Penicillium, among
others
[0137] Antibodies directed against target antigens associated with
protozoa include, but are not limited to, antibodies associated
with Trypanosoma, Leishmania species including Leishmania
donovanii; Plasmodium spp., Pneumocystis carinii, Cryptosporidium
parvum, Giardia lamblia, Entamoeba histolytica, and Cyclospora
cayetanensis.
[0138] Antibodies against prokaryotic antigens are also useful,
including antibodies against suitable bacteria such as pathogenic
and non-pathogenic prokaryotes including but not limited to
Bacillus, including Bacillus anthracis; Vibrio, e.g. V. cholerae;
Escherichia, e.g. Enterotoxigenic E. coli, Shigella, e.g. S.
dysenteriae; Salmonella, e.g. S. typhi; Mycobacterium e.g. M.
tuberculosis, M. leprae; Clostridium, e.g. C. botulinum, C. tetani,
C. difficile, C.perfringens; Cornyebacterium, e.g. C. diphtheriae;
Streptococcus, S. pyogenes, S. pneumoniae; Staphylococcus, e.g. S.
aureus; Haemophilus, e.g. H. influenzae; Neisseria, e.g. N.
meningitidis, N. gonorrhoeae; Yersinia, e.g. Y. lamblia, Y. pestis,
Pseudomonas, e.g. P. aeruginosa, P. putida; Chlamydia, e.g. C.
trachomatis; Bordetella, e.g. B. pertussis; Treponema, e.g. T.
palladium; B. anthracis, Y. pestis, Brucella spp., F. tularensis,
B. mallei, B. pseudomallei, B. mallei, B. pseudomallei, C.
botulinum, Salmonella spp., SEB V. cholerae toxin B, E. coli
O157:H7, Listeria spp., Trichosporon beigelii, Rhodotorula species,
Hansenula anomala, Enterobacter sp., Klebsiella sp., Listeria sp.,
Mycoplasma sp. and the like.
[0139] In some aspects, the antibodies are directed against viral
infections; these viruses include, but are not limited to,
including orthomyxoviruses, (e.g. influenza virus), paramyxoviruses
(e.g respiratory syncytial virus, mumps virus, measles virus),
adenoviruses, rhinoviruses, coronaviruses, reoviruses, togaviruses
(e.g. rubella virus), parvoviruses, poxviruses (e.g. variola virus,
vaccinia virus), enteroviruses (e.g. poliovirus, coxsackievirus),
hepatitis viruses (including A, B and C), herpesviruses (e.g.
Herpes simplex virus, varicella-zoster virus, cytomegalovirus,
Epstein-Barr virus), rotaviruses, Norwalk viruses, hantavirus,
arenavirus, rhabdovirus (e.g. rabies virus), retroviruses
(including HIV, HTLV-I and -II), papovaviruses (e.g.
papillomavirus), polyomaviruses, and picornaviruses, and the
like.
[0140] Optimized IgG Variant Properties
[0141] The present application also provides IgG variants that are
optimized for a variety of therapeutically relevant properties. An
IgG variant that is engineered or predicted to display one or more
optimized properties is herein referred to as an "optimized IgG
variant". The most preferred properties that may be optimized
include but are not limited to enhanced or reduced affinity for
FcRn and increased or decreased in vivo half-life. Suitable
embodiments include antibodies that exhibit increased binding
affinity to FcRn at lowered pH, such as the pH associated with
endosomes, e.g. pH 6.0, while maintaining the reduced affinity at
higher pH, such as 7.4., to allow increased uptake into endosomes
but normal release rates. Similarly, these antibodies with
modulated FcRn binding may optionally have other desirable
properties, such as modulated Fc.gamma.R binding, such as outlined
in U.S. Ser. Nos. U.S. Ser. Nos. 11/174,287, 11/124,640,
10/822,231, 10/672,280, 10/379,392, and the patent application
entitled IgG Immunoglobulin variants with optimized effector
function filed on Oct. 21, 2005 having application Ser. No.
11/256,060. That is, optimized properties also include but are not
limited to enhanced or reduced affinity for an Fc.gamma.R. In one
optional embodiment, the IgG variants are optimized to possess
enhanced affinity for a human activating Fc.gamma.R, preferably
Fc.gamma.RIIIa in addition to the FcRn binding profile. In yet
another optional alternate embodiment, the IgG variants are
optimized to possess reduced affinity for the human inhibitory
receptor Fc.gamma.RIIb. That is, particular embodiments embrace the
use of antibodies that show increased binding to FcRn, and
increased binding to Fc.gamma.RIIIa. Other embodiments utilize use
of antibodies that show increased binding to FcRn, and increased
binding to Fc.gamma.RIIIa. These embodiments are anticipated to
provide IgG polypeptides with enhanced therapeutic properties in
humans, for example enhanced effector function and greater
anti-cancer potency. In an alternate embodiment, the IgG variants
are optimized to have increased or reduced affinity for FcRn and
increased or decreased affinity for a human Fc.gamma.R, including
but not limited to Fc.gamma.RI, Fc.gamma.RIIa, Fc.gamma.RIIb,
Fc.gamma.RIIc, Fc.gamma.RIIIa, and Fc.gamma.RIIIb including their
allelic variations. These embodiments are anticipated to provide
IgG polypeptides with enhanced therapeutic properties in humans,
for example increased serum half-life and reduced effector
function. In other embodiments, IgG variants provide enhanced
affinity for FcRn and enhanced affinity for one or more
Fc.gamma.Rs, yet reduced affinity for one or more other
Fc.gamma.Rs. For example, an IgG variant may have enhanced binding
to FcRn and Fc.gamma.RIIIa, yet reduced binding to Fc.gamma.RIIb.
Alternately, an IgG variant may have reduced binding to FcRn and to
Fc.gamma.R's. In another embodiment, an IgG variant may have
reduced affinity for FcRn and enhanced affinity for Fc.gamma.RIIb,
yet reduced affinity to one or more activating Fc.gamma.Rs. In yet
another embodiment, an IgG variant may have increased serum
half-life and reduced effector functions.
[0142] Preferred embodiments comprise optimization of binding to a
human FcRn and Fc.gamma.R, however in alternate embodiments the IgG
variants possess enhanced or reduced affinity for FcRn and
Fc.gamma.R from nonhuman organisms, including but not limited to
rodents and non-human primates. IgG variants that are optimized for
binding to a nonhuman FcRn may find use in experimentation. For
example, mouse models are available for a variety of diseases that
enable testing of properties such as efficacy, toxicity, and
pharmacokinetics for a given drug candidate. As is known in the
art, cancer cells can be grafted or injected into mice to mimic a
human cancer, a process referred to as xenografting. Testing of IgG
variants that comprise IgG variants that are optimized for FcRn may
provide valuable information with regard to the clearance
characteristics of the protein, its mechanism of clearance, and the
like. The IgG variants may also be optimized for enhanced
functionality and/or solution properties in aglycosylated form. The
Fc ligands include but are not limited to FcRn, Fc.gamma.Rs, C1q,
and proteins A and G, and may be from any source including but not
limited to human, mouse, rat, rabbit, or monkey, preferably human.
In an alternately preferred embodiment, the IgG variants are
optimized to be more stable and/or more soluble than the
aglycosylated form of the parent IgG variant.
[0143] IgG variants can include modifications that modulate
interaction with Fc ligands other than FcRn and Fc.gamma.Rs,
including but not limited to complement proteins, and Fc receptor
homologs (FcRHs). FcRHs include but are not limited to FcRH1,
FcRH2, FcRH3, FcRH4, FcRH5, and FcRH6 (Davis et al., 2002, Immunol.
Reviews 190:123-136, entirely incorporated by reference).
[0144] Preferably, the Fc ligand specificity of the IgG variant
will determine its therapeutic utility. The utility of a given IgG
variant for therapeutic purposes will depend on the epitope or form
of the target antigen and the disease or indication being treated.
For most targets and indications, enhanced FcRn binding may be
preferable as the enhanced FcRn binding may result in an increase
in serum half-life. Longer serum half-lives allow less frequent
dosing or lower dosing of the therapeutic. This is particularly
preferable when the therapeutic agent is given in response to an
indication that requires repeated administration. For some targets
and indications, decreased FcRn affinity may be preferable. This
may be particularly preferable when a variant Fc with increased
clearance or decreased serum half-life is desired, for example in
Fc polypeptides used as imaging agents or radio-therapeutics.
[0145] IgG variants may be used that comprise IgG variants that
provide enhanced affinity for FcRn with enhanced activating
Fc.gamma.Rs and/or reduced affinity for inhibitory Fc.gamma.Rs. For
some targets and indications, it may be further beneficial to
utilize IgG variants that provide differential selectivity for
different activating Fc.gamma.Rs; for example, in some cases
enhanced binding to Fc.gamma.RIIa and Fc.gamma.RIIIa may be
desired, but not Fc.gamma.RI, whereas in other cases, enhanced
binding only to Fc.gamma.RIIa may be preferred. For certain targets
and indications, it may be preferable to utilize IgG variants that
alter FcRn binding and enhance both Fc.gamma.R-mediated and
complement-mediated effector functions, whereas for other cases it
may be advantageous to utilize IgG variants that enhance FcRn
binding, or serum half-life, and either Fc.gamma.R-mediated or
complement-mediated effector functions. For some targets or cancer
indications, it may be advantageous to reduce or ablate one or more
effector functions, for example by knocking out binding to C1q, one
or more Fc.gamma.R's, FcRn, or one or more other Fc ligands. For
other targets and indications, it may be preferable to utilize IgG
variants that provide enhanced binding to the inhibitory
Fc.gamma.RIIb, yet WT level, reduced, or ablated binding to
activating Fc.gamma.Rs. This may be particularly useful, for
example, when the goal of an IgG variant is to inhibit inflammation
or auto-immune disease, or modulate the immune system in some way.
Because auto-immune diseases are generally long-lasting and
treatment is given in repeated dosing, their treatment with Fc
variants with increased half-life from increased FcRn is
particularly preferred.
[0146] Modification may be made to improve the IgG stability,
solubility, function, or clinical use. In a preferred embodiment,
the IgG variants can include modifications to reduce immunogenicity
in humans. In a most preferred embodiment, the immunogenicity of an
IgG variant is reduced using a method described in U.S. Ser. No.
11/004,590, entirely incorporated by reference. In alternate
embodiments, the IgG variants are humanized (Clark, 2000, Immunol
Today 21:397-402, entirely incorporated by reference).
[0147] The IgG variants can include modifications that reduce
immunogenicity. Modifications to reduce immunogenicity can include
modifications that reduce binding of processed peptides derived
from the parent sequence to MHC proteins. For example, amino acid
modifications would be engineered such that there are no or a
minimal number of immune epitopes that are predicted to bind, with
high affinity, to any prevalent MHC alleles. Several methods of
identifying MHC-binding epitopes in protein sequences are known in
the art and may be used to score epitopes in an IgG variant. See
for example WO 98/52976; WO 02/079232; WO 00/3317; U.S. Ser. NO.
09/903,378; U.S. Ser. No. 10/039,170; U.S. Ser. No. 60/222,697;
U.S. Ser. No. 10/754,296; PCT WO 01/21823; and PCT WO 02/00165;
Mallios, 1999, Bioinformatics 15: 432-439; Mallios, 2001,
Bioinformatics 17: 942-948; Sturniolo et al., 1999, Nature Biotech.
17: 555-561; WO 98/59244; WO 02/069232; WO 02/77187; Marshall et
al., 1995, J. Immunol. 154: 5927-5933; and Hammer et al., 1994, J.
Exp. Med. 180: 2353-2358, all entirely incorporated by reference.
Sequence-based information can be used to determine a binding score
for a given peptide--MHC interaction (see for example Mallios,
1999, Bioinformatics 15: 432-439; Mallios, 2001, Bioinformatics 17:
p942-948; Sturniolo et. al., 1999, Nature Biotech. 17: 555-561, all
entirely incorporated by reference).
[0148] Engineering IgG Variants
[0149] Variants of the present invention may be designed by various
means. The variants, as described herein, may be insertions,
deletions, substitutions, other modifications, or combinations of
these and other changes. A particularly novel embodiment of the
present invention is the design of insertions and deletions that
either improve or reduce the binding of an Fc polypeptide to an Fc
ligand. As disclosed herein, insertions or deletions may be made
that increase or decrease the affinity of the Fc polypeptide for
FcRn. Insertions and deletions may be designed by rational
approaches or by approaches that include the use or random
components, such as random or semi-random library creation or
screening. In an alternative embodiment, substitutions are
disclosed that increase or decrease the affinity of the Fc
polypeptide for FcRn.
[0150] Backbone Modifications: Insertions and Deletions
[0151] Variant Fc polypeptides may be created by substituting a
variant amino acid in place of the parent amino acid at a position
in the Fc polypeptide. By substituting one or more amino acids for
variant amino acids in the Fc polypeptide, the side chains at those
positions are altered. Most useful substitutions modify the Fc
properties by altering the Fc side chains. The substituted side
chains may interact directly or indirectly with an Fc binding
partner that is associated with an Fc function or property. The at
least one substitution alters the covalent structure of one or more
side chains of the parent Fc polypeptide.
[0152] Alternatively, variant Fc polypeptides may be created that
change the covalent structure of the backbone of the parent Fc
polypeptide. The backbone atoms in proteins are the peptide
nitrogen, the alpha carbon, the carbonyl or peptide carbon and the
carbonyl oxygen. Changing the covalent structure of the backbone
provides additional methods of altering the properties of the Fc
polypeptides. The covalent structure of the Fc backbone may be
altered by the addition of atoms into the backbone, e.g. by
inserting one or more amino acids, or the subtraction of atoms from
the backbone, e.g. by deleting one or more amino acids. The
covalent structure of the backbone may also be altered by changing
individual atoms of the backbone to other atoms (Deechongkit et
al., J Am Chem Soc. 2004. 126(51):16762-71, entirely incorporated
by reference). As is known in the art and is illustrated herein,
insertions or deletions of amino acids in Fc polypeptides may be
done by inserting or deleting the corresponding nucleotides in the
DNA encoding the Fc polypeptide. Alternatively, as is known in the
art, insertions or deletions of amino acids may be done during
synthesis of Fc polypeptides.
[0153] The design of insertions or deletions of amino acids that
alter the interaction of the Fc polypeptide with one or more
binding partners (e.g. FcgammaR's, FcRn, C1q) may be done by
considering the structure of the complex of the Fc polypeptide and
its binding partner. In a less preferred embodiment, the design may
be done by considering the structure of the Fc polypeptide and
information about the Fc region involved in binding the binding
partner. This information may be obtained by mutagenesis
experiments, phage display experiments, homology comparisons,
computer modeling or other means.
[0154] Preferred positions in the amino acid sequence for
insertions or deletions that affect the Fc binding interactions,
but do not affect the overall structure, stability, expression or
use of the Fc polypeptide, are in loops that are involved in the
Fc/Fc-binding partner interactions. To alter FcRn binding to the Fc
polypeptide, positions 244-257, 279-284, 307-317, 383-390, and
428-435 are preferred loop locations for insertions or deletions
(numbering from EU index of Kabat et al., Burmeister et al., 1994,
Nature, 372:379-383; Martin et al., 2001, Mol Cell 7:867-877, all
entirely incorporated by reference). To alter the Fcgamma receptor
binding to the Fc polypeptide, positions 229-239, 266-273, 294-299,
and 324-331 are preferred loop locations for insertions or
deletions (numbering from EU index of Kabat et al., PDB code 1
E4K.pdb Sondermann et al. Nature. 2000 406:267, all entirely
incorporated by reference). Loops are regions of the polypeptide
not involved in alpha helical or beta sheet structure. Loops
positions are positions that are not in either alpha helical or
beta sheet structures (van Holde, Johnson and Ho. Principles of
Physical Biochemistry. Prentice Hall, N.J. 1998, Chapter 1 pp2-67,
entirely incorporated by reference). Loop positions are preferred
because the backbone atoms are typically more flexible and less
likely involved in hydrogen bonds compared to the backbone atoms of
alpha helices and beta sheets. Therefore, the lengthening or
shortening of a loop due to an insertion or deletion of one or more
amino acids is less likely to lead to large, disruptive changes to
the Fc polypeptide, including stability, expression or other
problems.
[0155] Insertions and deletions may be used to alter the length of
the polypeptide. For example, in loop regions, altering the loop
length results in altered flexibility and conformational entropy of
the loop. Insertions in a loop will generally increase the
conformational entropy of the loop, which may be defined as
Boltzman's constant multiplied by the natural logarithm of the
number of possible conformations (van Holde, Johnson and Ho.
Principles of Physical Biochemistry. Prentice Hall, New Jersey
1998, pp78, entirely incorporated by reference). By inserting at
least one amino acid into a polypeptide, the total number of
conformations available to the polypeptide increases. These
additional conformations may be beneficial for forming favorable
Fc/Fc-binding partner interactions because the Fc polypeptide may
use one of the additional conformations in binding the Fc-binding
protein. In this case, the insertion may lead to stronger
Fc/Fc-binding partner interactions. If the additional conformations
are not used in the binding interface, then the insertion may lead
to weaker Fc/Fc-binding partner interactions, because the
additional conformations would compete with the binding-competent
conformation. Similarly, deletion of a polypeptide segment may also
lead to either stronger or weaker Fc/Fc binding-partner
interactions. If deletion of a segment, which reduces the possible
number of backbone conformations, removes the binding-competent
conformation, then the deletion may lead to weaker Fc/Fc-binding
partner interactions. If the deletion does not remove the
binding-competent conformation, then the deletion may lead to
stronger Fc/Fc-binding partner interactions because the deletion
may remove conformations that compete with the binding-competent
conformation.
[0156] Insertions and deletions may be used to alter the positions
and orientations of the amino acids in the Fc polypeptide. Because
insertions and deletions cause a change in the covalent structure
of the backbone, they necessarily cause a change in the positions
of the backbone atoms. FIG. 7 compares the backbone positions at
some loop segments, marked L1 to L4, in three different backbones.
The reference backbone structure contains four loop segments,
whereas the deletion backbone lacks segment L1 and the insertion
segment comprises an additional segment before, ie, N-terminal to,
segment L1. Deletions and insertions cause the largest change in
the backbone structure near the site of the insertion or deletion.
By deleting a segment near the N-terminal end of the loop, e.g.
segment L1, the loop shortens and the remaining segments shift
their position closer to the loop N-terminus. This has the effect
of moving the L2 segment toward the prior location of the L1
segment and toward the loop N-terminus. This change in position of
the L2 segment toward the L1 segment may strengthen the binding of
the Fc/Fc-binding partner complex and is preferred when there is
prior information suggesting that the amino acid or amino acids
located in L2 make favorable interactions with the Fc-binding
partner, when located in L1. For example, if L2 contains alanine
and tyrosine and substitution of two L1 amino acids for alanine and
tyrosine previously lead to an Fc variant with increased binding,
then deletion of L1 may create an Fc variant with increased
affinity for the Fc-binding partner.
[0157] Similarly, an insertion of a polypeptide segment into an Fc
polypeptide at the N-terminal side of a loop causes the positions
of the loop segments to be shifted toward the C-terminal side of
the loop. In FIG. 7, an insertion of one or more amino acids
before, i.e. N-terminally to, segment L1 alters the backbone
conformation including a shift of the L1 segment toward the
C-terminal end of the loop. This type of insertion is preferred
when the amino acids located in segment L1 are known to make
favorable interactions when located in the L2 positions, as the
insertion may lead to stronger Fc/Fc-binding partner interactions.
If weaker Fc/Fc-binding partner interactions are desired, then the
insertion may be used to shift unfavorable amino acid into a new
position. The inserted, deleted and reference segments (L1 to L4 in
FIG. 7) may be one or more than one amino acid in the Fc
polypeptide.
[0158] Alternatively, insertions or deletions may be used at the
C-terminal end of loops in a manner analogous to the insertions or
deletions at the N-terminal end of loops. Insertions at the loop
C-terminus may lead to a movement of the positions N-terminal of
the insertion toward the loop N-terminus. Deletions at the loop
C-terminus may lead to a movement of the positions N-terminal of
the deletion toward the loop C-terminus. The choice of using an
insertion or deletion at the N-terminal or C-terminal end of the
loop is based on the amino acids located in the loop, the desire
for increased or decreased Fc/Fc-binding partner affinity, and the
positional shift desired.
[0159] Insertions or deletions may be used in any region of an Fc
polypeptide, including the loops, the alpha helical, and the beta
sheet regions. Preferred locations for insertions and deletions
include loop regions, which are those that are not alpha helical or
beta sheet regions. Loops are preferred because they generally
accept alterations in the backbone better than alpha helixes or
beta sheets. The particularly preferred locations for insertions or
deletions that result in stronger protein/protein interactions are
at the N-terminal or C-terminal edges of a loop. If the loop side
chains are involve in the Fc/Fc-binding partner interactions, then
insertions or deletion at the edges are less likely to lead to
strongly detrimental changes in the binding interactions. Deletions
within the exact center of the loop are more likely to remove
important residues in the Fc/Fc-binding partner interface and
insertions within the exact center of the loop are more likely to
create unfavorable interactions in the Fc/Fc-binding partner
interface. The number of residues deleted or inserted may be
determined by the size of the backbone change desired with
insertions or deletions of 15 or less residues being preferred,
insertions or deletions of 10 or less residues being more
preferred, and insertions or deletions of 5 or less residues being
most preferred.
[0160] Once the position and size of an Fc deletion variant is
designed, the entire polypeptide sequence is completely determined
and the polypeptide may be constructed by methods known in the
art.
[0161] Fc insertion variants, however, have the additional step of
designing the sequence of the at least one amino acid to be
inserted. Insertions of polar residues, including Ser, Thr, Asn,
Gln, Ala, Gly, His, are preferred at positions expected to be
exposed in the Fc polypeptide. The smaller amino acids, including
Ser, Thr, and Ala, are particularly preferred as the small size is
less likely to sterically interfere with the Fc/Fc-binding partner
interactions. Ser and Thr also have the capability to hydrogen bond
with atoms on the Fc-binding partner.
[0162] Insertions also have the added flexibility that the inserted
polypeptide may be designed to make favorable interactions with the
Fc-binding partner as would be desire when stronger Fc/Fc-binding
partner binding is desired. The length of the backbone insertion
may be determined by modeling the variant backbone with a simple,
generic sequence to be inserted. For example, polyserine,
polyglycine or polyalanine insertions of different lengths may be
constructed and modeled. Modeling may be done by a variety of
methods, including homology modeling based on known
three-dimensional structures of homologues comprising the
insertion, and by computer modeling including MODELLER (M. A.
Marti-Renom et al. Annu. Rev. Biophys. Biomol. Struct. 29, 291-325,
2000) and ROSETTA (Kuhlman et al. (2003). Science 302, 1364-8),
both entirely incorporated by reference. Typically, various
backbone conformations are initially generated and the final
backbone structure may be determined after the identities of the
side chain are established. The side chains may be designed by
PDA.RTM. algorithms (U.S. Pat. Nos. 6,188,965; 6,269,312;
6,403,312; 6,801,861; 6,804,611; 6,792,356, 6,950,754, and U.S.
Ser. Nos. 09/782,004; 09/927,790; 10/101,499; 10/666,307;
10/666311; 10/218,102, all entirely incorporated by reference).
[0163] Insertions and deletions may be made to alter the binding of
Fc polypeptides to FcgammaR in an analogous manner to the described
method to alter FcRn-binding properties. Fc domains bind to the
FcgammaR at the position indicated in FIG. 1. Structures of the
Fc/FcgammaR complex, including PDB codes 1T89 and 1IIS (Radaev Set
al. J. Biol. Chem. v276, p.16469-16477 entirely incorporated by
reference), demonstrate the interacting residues and loops between
the two structures. Mutagenesis results such as those found in U.S.
Ser. No. 11/124620 and U.S. Pat. No. 6,737,056, both entirely
incorporated by reference) all have utility in determined
appropriate shifts of backbone positioning.
[0164] Insertions and deletions may be designed in any polypeptide
besides Fc polypeptides by the methods described herein. For
example, insertions or deletions in the TNF superfamily member,
APRIL, may be designed with the aid of its three-dimensional
structure (PDB code 1XU1.pdb, Hymowitz, et al. (2005) J. Biol.
Chem. 280:7218, entirely incorporated by reference). Insertions or
deletions may be designed to increase APRIL binding to its
receptor, TACI. The loop residues preferred as insertion or
deletion sites are residues Ser118-Val124, Asp164-Phe167,
Pro192-Ala198, Pro221-Lys226. These loops interact with TACI in the
APRIL/TACI complex and mediate binding.
[0165] Polypeptides Incorporating Variants
[0166] The IgG variants can be based on human IgG sequences, and
thus human IgG sequences are used as the "base" sequences against
which other sequences are compared, including but not limited to
sequences from other organisms, for example rodent and primate
sequences. IgG variants may also comprise sequences from other
immunoglobulin classes such as IgA, IgE, IgD, IgM, and the like. It
is contemplated that, although the IgG variants are engineered in
the context of one parent IgG, the variants may be engineered in or
"transferred" to the context of another, second parent IgG. This is
done by determining the "equivalent" or "corresponding" residues
and substitutions between the first and second IgG, typically based
on sequence or structural homology between the sequences of the
IgGs. In order to establish homology, the amino acid sequence of a
first IgG outlined herein is directly compared to the sequence of a
second IgG. After aligning the sequences, using one or more of the
homology alignment programs known in the art (for example using
conserved residues as between species), allowing for necessary
insertions and deletions in order to maintain alignment (i.e.,
avoiding the elimination of conserved residues through arbitrary
deletion and insertion), the residues equivalent to particular
amino acids in the primary sequence of the first IgG variant are
defined. Alignment of conserved residues preferably should conserve
100% of such residues. However, alignment of greater than 75% or as
little as 50% of conserved residues is also adequate to define
equivalent residues. Equivalent residues may also be defined by
determining structural homology between a first and second IgG that
is at the level of tertiary structure for IgGs whose structures
have been determined. In this case, equivalent residues are defined
as those for which the atomic coordinates of two or more of the
main chain atoms of a particular amino acid residue of the parent
or precursor (N on N, CA on CA, C on C and O on O) are within 0.13
nm and preferably 0.1 nm after alignment. Alignment is achieved
after the best model has been oriented and positioned to give the
maximum overlap of atomic coordinates of non-hydrogen protein atoms
of the proteins. Regardless of how equivalent or corresponding
residues are determined, and regardless of the identity of the
parent IgG in which the IgGs are made, what is meant to be conveyed
is that the IgG variants discovered by can be engineered into any
second parent IgG that has significant sequence or structural
homology with the IgG variant. Thus for example, if a variant
antibody is generated wherein the parent antibody is human IgG1, by
using the methods described above or other methods for determining
equivalent residues, the variant antibody may be engineered in
another IgG1 parent antibody that binds a different antigen, a
human IgG2 parent antibody, a human IgA parent antibody, a mouse
IgG2a or IgG2b parent antibody, and the like. Again, as described
above, the context of the parent IgG variant does not affect the
ability to transfer the IgG variants to other parent IgGs.
[0167] Methods for engineering, producing, and screening IgG
variants are provided. The described methods are not meant to
constrain to any particular application or theory of operation.
Rather, the provided methods are meant to illustrate generally that
one or more IgG variants may be engineered, produced, and screened
experimentally to obtain IgG variants with optimized effector
function. A variety of methods are described for designing,
producing, and testing antibody and protein variants in U.S. Ser.
No. 10/754,296, and U.S. Ser. No. 10/672,280, both entirely
incorporated by reference.
[0168] A variety of protein engineering methods may be used to
design IgG variants with optimized effector function. In one
embodiment, a structure-based engineering method may be used,
wherein available structural information is used to guide
substitutions, insertions or deletions. In a preferred embodiment,
a computational screening method may be used, wherein substitutions
are designed based on their energetic fitness in computational
calculations. See for example U.S. Ser. No. 10/754,296 and U.S.
Ser. No. 10/672,280, and references cited therein, all entirely
incorporated by reference.
[0169] An alignment of sequences may be used to guide substitutions
at the identified positions. One skilled in the art will appreciate
that the use of sequence information may curb the introduction of
substitutions that are potentially deleterious to protein
structure. The source of the sequences may vary widely, and include
one or more of the known databases, including but not limited to
the Kabat database (Northwestern University); Johnson & Wu,
2001, Nucleic Acids Res. 29:205-206; Johnson & Wu, 2000,
Nucleic Acids Res. 28:214-218), the IMGT database (IMGT, the
international ImMunoGeneTics information system.RTM.; Lefranc et
al., 1999, Nucleic Acids Res. 27:209-212; Ruiz et al., 2000 Nucleic
Acids Res. 28:219-221; Lefranc et al., 2001, Nucleic Acids Res.
29:207-209; Lefranc et al., 2003, Nucleic Acids Res. 31:307-310),
and VBASE, all entirely incorporated by reference. Antibody
sequence information can be obtained, compiled, and/or generated
from sequence alignments of germline sequences or sequences of
naturally occurring antibodies from any organism, including but not
limited to mammals. One skilled in the art will appreciate that the
use of sequences that are human or substantially human may further
have the advantage of being less immunogenic when administered to a
human. Other databases which are more general nucleic acid or
protein databases, i.e. not particular to antibodies, include but
are not limited to SwissProt, GenBank Entrez, and EMBL Nucleotide
Sequence Database. Aligned sequences can include VH, VL, CH, and/or
CL sequences. There are numerous sequence-based alignment programs
and methods known in the art, and all of these find use in the
generation of sequence alignments.
[0170] Alternatively, random or semi-random mutagenesis methods may
be used to make amino acid modifications at the desired positions.
In these cases positions are chosen randomly, or amino acid changes
are made using simplistic rules. For example all residues may be
mutated to alanine, referred to as alanine scanning. Such methods
may be coupled with more sophisticated engineering approaches that
employ selection methods to screen higher levels of sequence
diversity. As is well known in the art, there are a variety of
selection technologies that may be used for such approaches,
including, for example, display technologies such as phage display,
ribosome display, cell surface display, and the like, as described
below.
[0171] Methods for production and screening of IgG variants are
well known in the art. General methods for antibody molecular
biology, expression, purification, and screening are described in
Antibody Engineering, edited by Duebel & Kontermann,
Springer-Verlag, Heidelberg, 2001; and Hayhurst & Georgiou,
2001, Curr Opin Chem Biol 5:683-689; Maynard & Georgiou, 2000,
Annu Rev Biomed Eng 2:339-76. Also see the methods described in
U.S. Ser. No. 10/754,296; U.S. Ser. No. 10/672,280; and U.S. Ser.
No. 10/822,231; and 11/124,620, all entirely incorporated by
reference. [0172] Preferred variants of the present invention
include those found in FIG. 8. Alternatively preferred variants of
the present invention include those found in FIG. 9. Additionally
alternatively preferred variants of the present invention include
those found in FIG. 10. These variants have shown increased binding
to the Fc receptor, FcRn, as illustrated in the examples.
[0173] Making IgG Variants
[0174] The IgG variants can be made by any method known in the art.
In one embodiment, the IgG variant sequences are used to create
nucleic acids that encode the member sequences, and that may then
be cloned into host cells, expressed and assayed, if desired. These
practices are carried out using well-known procedures, and a
variety of methods that may find use in are described in Molecular
Cloning--A Laboratory Manual, 3.sup.rd Ed. (Maniatis, Cold Spring
Harbor Laboratory Press, New York, 2001), and Current Protocols in
Molecular Biology (John Wiley & Sons), both entirely
incorporated by reference. The nucleic acids that encode the IgG
variants may be incorporated into an expression vector in order to
express the protein. Expression vectors typically include a protein
operably linked, that is, placed in a functional relationship, with
control or regulatory sequences, selectable markers, any fusion
partners, and/or additional elements. The IgG variants may be
produced by culturing a host cell transformed with nucleic acid,
preferably an expression vector, containing nucleic acid encoding
the IgG variants, under the appropriate conditions to induce or
cause expression of the protein. A wide variety of appropriate host
cells may be used, including but not limited to mammalian cells,
bacteria, insect cells, and yeast. For example, a variety of cell
lines that may find use are described in the ATCC cell line
catalog, available from the American Type Culture Collection,
entirely incorporated by reference. The methods of introducing
exogenous nucleic acid into host cells are well known in the art,
and will vary with the host cell used.
[0175] In a preferred embodiment, IgG variants are purified or
isolated after expression. Antibodies may be isolated or purified
in a variety of ways known to those skilled in the art. Standard
purification methods include chromatographic techniques,
electrophoretic, immunological, precipitation, dialysis,
filtration, concentration, and chromatofocusing techniques. As is
well known in the art, a variety of natural proteins bind
antibodies, for example bacterial proteins A, G, and L, and these
proteins may find use in purification. Often, purification may be
enabled by a particular fusion partner. For example, proteins may
be purified using glutathione resin if a GST fusion is employed,
Ni.sup.+2 affinity chromatography if a His-tag is employed, or
immobilized anti-flag antibody if a flag-tag is used. For general
guidance in suitable purification techniques, see Antibody
Purification: Principles and Practice, 3.sup.rd Ed., Scopes,
Springer-Verlag, N.Y., 1994, entirely incorporated by
reference.
[0176] Screening IgG Variants
[0177] Fc variants may be screened using a variety of methods,
including but not limited to those that use in vitro assays, in
vivo and cell-based assays, and selection technologies. Automation
and high-throughput screening technologies may be utilized in the
screening procedures. Screening may employ the use of a fusion
partner or label, for example an immune label, isotopic label, or
small molecule label such as a fluorescent or colorimetric dye.
[0178] In a preferred embodiment, the functional and/or biophysical
properties of Fc variants are screened in an in vitro assay. In a
preferred embodiment, the protein is screened for functionality,
for example its ability to catalyze a reaction or its binding
affinity to its target.
[0179] As is known in the art, subsets of screening methods are
those that select for favorable members of a library. The methods
are herein referred to as "selection methods", and these methods
find use in the present invention for screening Fc variants. When
protein libraries are screened using a selection method, only those
members of a library that are favorable, that is which meet some
selection criteria, are propagated, isolated, and/or observed. A
variety of selection methods are known in the art that may find use
in the present invention for screening protein libraries. Other
selection methods that may find use in the present invention
include methods that do not rely on display, such as in vivo
methods. A subset of selection methods referred to as "directed
evolution" methods are those that include the mating or breading of
favorable sequences during selection, sometimes with the
incorporation of new mutations.
[0180] In a preferred embodiment, Fc variants are screened using
one or more cell-based or in vivo assays. For such assays, purified
or unpurified proteins are typically added exogenously such that
cells are exposed to individual variants or pools of variants
belonging to a library. These assays are typically, but not always,
based on the function of the Fc polypeptide; that is, the ability
of the Fc polypeptide to bind to its target and mediate some
biochemical event, for example effector function, ligand/receptor
binding inhibition, apoptosis, and the like. Such assays often
involve monitoring the response of cells to the IgG, for example
cell survival, cell death, change in cellular morphology, or
transcriptional activation such as cellular expression of a natural
gene or reporter gene. For example, such assays may measure the
ability of Fc variants to elicit ADCC, ADCP, or CDC. For some
assays additional cells or components, that is in addition to the
target cells, may need to be added, for example example serum
complement, or effector cells such as peripheral blood monocytes
(PBMCs), NK cells, macrophages, and the like. Such additional cells
may be from any organism, preferably humans, mice, rat, rabbit, and
monkey. Antibodies may cause apoptosis of certain cell lines
expressing the target, or they may mediate attack on target cells
by immune cells which have been added to the assay. Methods for
monitoring cell death or viability are known in the art, and
include the use of dyes, immunochemical, cytochemical, and
radioactive reagents. Transcriptional activation may also serve as
a method for assaying function in cell-based assays. Alternatively,
cell-based screens are performed using cells that have been
transformed or transfected with nucleic acids encoding the
variants. That is, Fc variants are not added exogenously to the
cells.
[0181] The biological properties of the IgG variants may be
characterized in cell, tissue, and whole organism experiments. As
is known in the art, drugs are often tested in animals, including
but not limited to mice, rats, rabbits, dogs, cats, pigs, and
monkeys, in order to measure a drug's efficacy for treatment
against a disease or disease model, or to measure a drug's
pharmacokinetics, toxicity, and other properties. The animals may
be referred to as disease models. Therapeutics are often tested in
mice, including but not limited to nude mice, SCID mice, xenograft
mice, and transgenic mice (including knockins and knockouts). Such
experimentation may provide meaningful data for determination of
the potential of the protein to be used as a therapeutic. Any
organism, preferably mammals, may be used for testing. For example
because of their genetic similarity to humans, monkeys can be
suitable therapeutic models, and thus may be used to test the
efficacy, toxicity, pharmacokinetics, or other property of the
IgGs. Tests of the in humans are ultimately required for approval
as drugs, and thus of course these experiments are contemplated.
Thus the IgGs may be tested in humans to determine their
therapeutic efficacy, toxicity, immunogenicity, pharmacokinetics,
and/or other clinical properties.
[0182] Methods of Using IgG Variants
[0183] The IgG variants may find use in a wide range of products.
In one embodiment the IgG variant is a therapeutic, a diagnostic,
or a research reagent, preferably a therapeutic. The IgG variant
may find use in an antibody composition that is monoclonal or
polyclonal. In a preferred embodiment, the IgG variants are used to
kill target cells that bear the target antigen, for example cancer
cells. In an alternate embodiment, the IgG variants are used to
block, antagonize or agonize the target antigen, for example for
antagonizing a cytokine or cytokine receptor. In an alternately
preferred embodiment, the IgG variants are used to block,
antagonize or agonize the target antigen and kill the target cells
that bear the target antigen.
[0184] The IgG variants may be used for various therapeutic
purposes. In a preferred embodiment, an antibody comprising the IgG
variant is administered to a patient to treat an antibody-related
disorder. A "patient" for the purposes includes humans and other
animals, preferably mammals and most preferably humans. By
"antibody related disorder" or "antibody responsive disorder" or
"condition" or "disease" herein are meant a disorder that may be
ameliorated by the administration of a pharmaceutical composition
comprising an IgG variant. Antibody related disorders include but
are not limited to autoimmune diseases, immunological diseases,
infectious diseases, inflammatory diseases, neurological diseases,
and oncological and neoplastic diseases including cancer. By
"cancer" and "cancerous" herein refer to or describe the
physiological condition in mammals that is typically characterized
by unregulated cell growth. Examples of cancer include but are not
limited to carcinoma, lymphoma, blastoma, sarcoma (including
liposarcoma), neuroendocrine tumors, mesothelioma, schwanoma,
meningioma, adenocarcinoma, melanoma, and leukemia and lymphoid
malignancies.
[0185] In one embodiment, an IgG variant is the only
therapeutically active agent administered to a patient.
Alternatively, the IgG variant is administered in combination with
one or more other therapeutic agents, including but not limited to
cytotoxic agents, chemotherapeutic agents, cytokines, growth
inhibitory agents, anti-hormonal agents, kinase inhibitors,
anti-angiogenic agents, cardioprotectants, or other therapeutic
agents. The IgG varariants may be administered concomitantly with
one or more other therapeutic regimens. For example, an IgG variant
may be administered to the patient along with chemotherapy,
radiation therapy, or both chemotherapy and radiation therapy. In
one embodiment, the IgG variant may be administered in conjunction
with one or more antibodies, which may or may not be an IgG
variant. In accordance with another embodiment, the IgG variant and
one or more other anti-cancer therapies are employed to treat
cancer cells ex vivo. It is contemplated that such ex vivo
treatment may be useful in bone marrow transplantation and
particularly, autologous bone marrow transplantation. It is of
course contemplated that the IgG variants can be employed in
combination with still other therapeutic techniques such as
surgery.
[0186] A variety of other therapeutic agents may find use for
administration with the IgG variants. In one embodiment, the IgG is
administered with an anti-angiogenic agent. By "anti-angiogenic
agent" as used herein is meant a compound that blocks, or
interferes to some degree, the development of blood vessels. The
anti-angiogenic factor may, for instance, be a small molecule or a
protein, for example an antibody, Fc fusion, or cytokine, that
binds to a growth factor or growth factor receptor involved in
promoting angiogenesis. The preferred anti-angiogenic factor herein
is an antibody that binds to Vascular Endothelial Growth Factor
(VEGF). In an alternate embodiment, the IgG is administered with a
therapeutic agent that induces or enhances adaptive immune
response, for example an antibody that targets CTLA-4. In an
alternate embodiment, the IgG is administered with a tyrosine
kinase inhibitor. By "tyrosine kinase inhibitor" as used herein is
meant a molecule that inhibits to some extent tyrosine kinase
activity of a tyrosine kinase. In an alternate embodiment, the IgG
variants are administered with a cytokine.
[0187] Pharmaceutical compositions are contemplated wherein an IgG
variant and one or more therapeutically active agents are
formulated. Formulations of the IgG variants are prepared for
storage by mixing the IgG having the desired degree of purity with
optional pharmaceutically acceptable carriers, excipients or
stabilizers (Remington's Pharmaceutical Sciences 16th edition,
Osol, A. Ed., 1980, entirely incorporated by reference), in the
form of lyophilized formulations or aqueous solutions. The
formulations to be used for in vivo administration are preferably
sterile. This is readily accomplished by filtration through sterile
filtration membranes or other methods. The IgG variants and other
therapeutically active agents disclosed herein may also be
formulated as immunoliposomes, and/or entrapped in
microcapsules.
[0188] The concentration of the therapeutically active IgG variant
in the formulation may vary from about 0.1 to 100% by weight. In a
preferred embodiment, the concentration of the IgG is in the range
of 0.003 to 1.0 molar. In order to treat a patient, a
therapeutically effective dose of the IgG variant may be
administered. By "therapeutically effective dose" herein is meant a
dose that produces the effects for which it is administered. The
exact dose will depend on the purpose of the treatment, and will be
ascertainable by one skilled in the art using known techniques.
Dosages may range from 0.01 to 100 mg/kg of body weight or greater,
for example 0.01, 0.1, 1.0, 10, or 50 mg/kg of body weight, with 1
to 10 mg/kg being preferred. As is known in the art, adjustments
for protein degradation, systemic versus localized delivery, and
rate of new protease synthesis, as well as the age, body weight,
general health, sex, diet, time of administration, drug interaction
and the severity of the condition may be necessary, and will be
ascertainable with routine experimentation by those skilled in the
art.
[0189] Administration of the pharmaceutical composition comprising
an IgG variant, preferably in the form of a sterile aqueous
solution, may be done in a variety of ways, including, but not
limited to, orally, subcutaneously, intravenously, parenterally,
intranasally, intraotically, intraocularly, rectally, vaginally,
transdermally, topically (e.g., gels, salves, lotions, creams,
etc.), intraperitoneally, intramuscularly, intrapulmonary (e.g.,
AERx.RTM. inhalable technology commercially available from Aradigm,
or Inhance.RTM. pulmonary delivery system commercially available
from Nektar Therapeutics, etc.). Therapeutic described herein may
be administered with other therapeutics concomitantly, i.e., the
therapeutics described herein may be co-administered with other
therapies or therapeutics, including for example, small molecules,
other biologicals, radiation therapy, surgery, etc.
EXAMPLES
[0190] Examples are provided below to illustrate the present
invention. These examples are not meant to constrain the present
invention to any particular application or theory of operation. For
all positions discussed in the present invention, numbering is
according to the EU index as in Kabat (Kabat et al., 1991,
Sequences of Proteins of Immunological Interest, 5th Ed., United
States Public Health Service, National Institutes of Health,
Bethesda, entirely incorporated by reference). Those skilled in the
art of antibodies will appreciate that this convention consists of
nonsequential numbering in specific regions of an immunoglobulin
sequence, enabling a normalized reference to conserved positions in
immunoglobulin families. Accordingly, the positions of any given
immunoglobulin as defined by the EU index will not necessarily
correspond to its sequential sequence.
Example 1
DNA Construction, Expression, and Purification of Fc Variants
[0191] Amino acid modifications were engineered in the Fc region of
IgG antibodies to improve their affinity for the neonatoal Fc
receptor FcRn. Variants were screened in the context of a number of
different human IgG constant chains (FIG. 2), including IgG1, IgG2,
and a hybrid IgG sequences that contains the CH1 and upper hinge of
IgG1 and the Fc region of IgG2. It will be appreciated by those
skilled in the art that because of the different interactions of
the IgG1 and IgG2 Fc region with Fc.gamma.Rs and complement, these
different parent Fc regions will have different Fc.gamma.R- and
complement-mediated effector function properties. Exemplary
sequences of Fc variants in the context of these parent IgG
constant chains are shown in FIG. 3.
[0192] Fc variants were engineered in the context of an antibody
targeting vascular endothelial factor (VEGF). The heavy and light
chain variable regions (VH and VL) are those of a humanized version
of the antibody A4.6.1, also referred to as bevacizumab
(Avastin.RTM.), which is approved for the treatment of a variety of
cancers. The amino acid sequences of the VH and VL regions of this
antibody are shown in FIG. 4.
[0193] Genes encoding the heavy and light chains of the anti-VEGF
antibodies were constructed in the mammalian expression vector
pTT5. Human IgG1 and IgG2 constant chain genes were obtained from
IMAGE clones and subcloned into the pTT5 vector. The IgG1/2 gene
was constructed using PCR mutagenesis. VH and VL genes encoding the
anti-VEGF antibodies were synthesized commercially (Blue Heron
Biotechnologies, Bothell Wash.), and subcloned into the vectors
encoding the appropriate CL, IgG1, IgG2, and IgG1/2 constant
chains. Amino acid modifications were constructed using
site-directed mutagenesis using the QuikChange.RTM. site-directed
mutagenesis methods (Stratagene, La Jolla Calif.). All DNA was
sequenced to confirm the fidelity of the sequences.
[0194] Plasmids containing heavy chain gene
(VH-C.gamma.1-C.gamma.2-C.gamma.3) were co-transfected with plasmid
containing light chain gene (VL-CK) into 293E cells using
llipofectamine (Invitrogen, Carlsbad Calif.) and grown in FreeStyle
293 media (Invitrogen, Carlsbad Calif.). After 5 days of growth,
the antibodies were purified from the culture supernatant by
protein A affinity using the MabSelect resin (GE Healthcare).
Antibody concentrations were determined by bicinchoninic acid (BCA)
assay (Pierce).
Example 2
Fc Variant Antibodies Maintain Binding to Antigen
[0195] The fidelity of the expressed variant antibodies was
confirmed by demonstrating that they maintained specificity for
antigen. VEGF binding was monitored using surface plasmon resonance
(SPR, Biacore), performed using a Biacore 3000 instrument.
Recombinant VEGF (VEGF-165, PeproTech, Rocky Hill, N.J.) was
adhered to a CM5 chip surface by coupling with
N-hydroxysuccinimide/N-ethyl-N'-(-3-dimethylamino-propyl)
carbodiimide (NHS/EDC) using standard methods. WT and variant
antibodies were injected as analytes, and response, measured in
resonance units (RU), was acquired. The dissociation phase was too
slow to measure true equilibrium constants, and so relative binding
was determined by measuring RU's at the end of the association
phase, which should be proportional to the protein concentration
(which is held constant in the experiment) and the association rate
constant. The data (FIG. 6) show that the variant anti-VEGF
antibodies maintain binding to antigen, in contrast to the negative
control anti-Her2 antibody which does not bind VEGF.
Example 3
Measurement of Binding to Human FcRn
[0196] Binding of variant antibodies to human FcRn was measured at
pH 6.0, the pH at which it is naturally bound in endosomes. Vectors
encoding beta 2 microglobulin and His-tagged alpha chain genes of
FcRn were constructed, co-transfected into 293T cells, and purified
using nickel chromatography. Antibody affinity for human FcRn
(hFcRn) at pH 6.0 was measured on a Biacore 3000 instrument by
coupling human FcRn to a CM5 chip surface using standard NHS/EDC
chemistry. WT and variant antibodies were used in the mobile phase
at 25-100 nM concentration and response was measured in resonance
units. Association and dissocation phases at pH 6.0 were acquired,
followed by an injection of pH 7.4 buffer to measure release of
antibody from receptor at the higher pH. A cycle with antibody and
buffer only provided baseline response, which was subtracted from
each sample sensorgram.
[0197] FIG. 7 shows Biacore sensorgrams for binding of native IgG1
and select Fc variant antibodies to human FcRn at the two relevant
pH's. The data show that wild-type and variant antibodies bind
readily to FcRn chip at pH 6.0 and dissociate slowly at that pH, as
they would in the endosome, yet release rapidly at pH7.4, as they
would upon recycling of endosome to the membrane and exposure to
the higher pH of serum.
[0198] The FcRn association/dissociation curves did not fit to a
simple Langmuir model, possibly due to the antibody and receptor
multi-valency or chip heterogeneity. Pseudo-Ka values (referred to
as Ka*) were determined by fitting to a conformational change model
with the change in refractive index (RI) fixed at 0 RU. These
values for select variant antibodies are plotted in FIG. 8. The
relative affinity of each variant as compared to its parent IgG was
calculated according to the equation Fold=(WT Ka*/Variant Ka*). The
relative binding data for all Fc variants in an IgG1 Fc region are
presented in FIG. 9, and binding data for variants in antibodies
with an IgG2 Fc region (constant chains IgG1 and IgG1/2) are
presented in FIG. 10. For many variants, the binding experiment was
repeated multiple times (n), for which folds were calculated with
reference to the WT IgG parent within each particular binding
experiment. Averaging of these data provided a mean and standard
deviation, as presented in FIGS. 9 and 10.
[0199] FIGS. 9 and 10 show that a number of engineered variants
bind with greater affinity to human FcRn binding at pH 6.0 relative
to WT IgG1. Improvements were heavily dependent on the identity of
the substitution at a given position. For example, using 2-fold as
a criteria for improved binding, a number of mutations at position
434 in IgG2 increased affinity (A, S, Y, F, and W), some were
neutral (within 2-fold of WT IgG2) (G, H, M, and T), and a number
of substitutions reduced affinity (<0.5 fold) (D, E, K, P, R,
and V). Greater binding in the context of IgG1 did not necessarily
translate to greater binding in IgG2 (for example 434T was improved
in binding in IgG1 but not IgG2). Moreover, improvements provided
by single variants were not always additive upon combination. FIG.
11a demonstrates this graphically by plotting the experimental fold
FcRn binding by select double substitution variants versus the
product of the fold FcRn binding by the individual single variants
that compose them. The straight line represents perfect additivity,
i.e. the value that would be expected or predicted from the product
of the single substitutions. A number of double variants fall on or
close to this line (259I/319I, 259I/428L, 319I/428L, and
308F/428L). Several variants are less than additive (319I/308F,
252Y/428L, and 428L/434M). For these variants, particularly in the
case of the latter two (252Y/428L and 428L/434M), the affinity
improvements of the single substitutions would seem to be
incompatable with each other when combined. Surprisingly, the FcRn
affinity improvements of variants 259I/308F and 428L/4345 were
greater than would be expected from the affinities of their
respective single substitutions. These particular single
substitutions had unexpected synergistic improvements when
combined. The difference between experimental affinities and those
predicted from the affinities of the single variants are plotted in
FIG. 11b, with variants grouped according to their composite single
variants (259I, 308F, and 319I on the left, and combinations with
482L on the right). Synergy can be quantitated by calculating the
fold of the experimental value relative to the predicted value,
followed by normalization to 1 and conversion to a percentage (%
synergy=100.times.[(experimental fold/predicted fold)-1)]. This
analysis is plotted in FIG. 11b, with variants grouped according to
their composite single variants. This graph highlights again the
synergy of some of the variants, particularly 259I/308F and
428L/4345. FIGS. 11b and 11c also emphasize the nonpredictive
nature of combining many of the best single substitutions from the
screen. For example, whereas combination of 428L with 434S and 259I
provided synergistic binding improvements, 252Y or 434M had a
negative impact when combined with 428L. The dramatic difference
between combining 428L with 434S versus 434M further highlights the
importance of the particular amino acid identity of the
substitution at a given position.
Example 4
Testing of Variants in Other Antibody Contexts
[0200] Select variants were constructed in the context of
antibodies targeting other antigens, including TNF (TNF.alpha.),
CD25 (TAC), EGFR, and IgE. FIG. 4 provides the amino acid sequences
of the VH and VL regions of antibodies targeting these antigens
that were used in the invention. The WT and Fc variant anti-TNF
antibodies contain the variable region of the fully human antibody
adalimumab (Humira.RTM.), currently approved for the treatment of
rheumatoid arthritis (RA), juvenile idiopathic arthritis (JIA),
psoriatic arthritis (PsA), ankylosing spondylitis (AS), and Crohn's
disease (CD). The WT and Fc variant anti-CD25 antibodies are
humanized versions of the antibody anti-TAC (Junghans et al., 1990,
Cancer Research 50:1495-1502), referred to as H1.8/L1 anti-TAC. The
WT and Fc variant anti-EGFR antibodies are humanized versions of
the murine antibody C225, referred to as H4.42/L3.32 C225. Finally,
the WT and Fc variant anti-IgE antibodies contain the variable
region of the humanized antibody omalizumab (Xolair.RTM.), which is
approved for the treatment of allergic asthma.
[0201] WT and variant antibodies were constructed, expressed, and
purified as described above. Antibodies were tested for binding to
human FcRn at pH 6.0 by Biacore as described above. The relative
binding data of the variant anti-TNF, -CD25, -EGFR, and -IgE
antibodies to human FcRn are provided in FIG. 12a-12d. As can be
seen, the variants improve FcRn affinity in the context of
antibodies targeting a variety of antigens.
Example 5
Pharmacokinetic Experiments in Human FcRn Knock-In Mice
[0202] To test the ability of select variants to improve half-life
in vivo, pharmacokinetic experiments were performed in B6 mice that
are homozygous knock-outs for murine FcRn and heterozygous
knock-ins of human FcRn (mFcRn.sup.-/-, hFcRn.sup.+) (Petkova et
al., 2006, Int Immunol 18(12):1759-69, entirely incorporated by
reference), herein referred to as hFcRn or hFcRn.sup.+ mice. A
single, intravenous tail vein injection of anti-VEGF antibody (2
mg/kg) was given to groups of 4-7 female mice randomized by body
weight (20-30 g range). Blood (.about.50 ul) was drawn from the
orbital plexus at each time point, processed to serum, and stored
at -80.degree. C. until analysis. Study durations were 28 or 49
days. Animals were not harmed during these studies.
[0203] Antibody concentrations were determined using two ELISA
assays. In the first two studies (referred to as Study 1 and Study
2), goat anti-human Fc antibody (Jackson Immuno research) was
adhered to the plate, wells were washed with PBST (phosphate
buffered saline with 0.05% Tween) and blocked with 3% BSA in PBST.
Serum or calibration standards were the added, followed by PBST
washing, addition of europium labeled anti-human IgG (Perkin
Elmer), and further PBST washing. The time resolved fluorescence
signal was collected. For Studies 3-5, serum concentration was
detected using a similar ELISA, but recombinant VEGF (VEGF-165,
PeproTech, Rocky Hill, N.J.) was used as capture reagent and
detection was carried out with biotinylated anti-human kappa
antibody and europium-labeled streptavidin. PK parameters were
determined for individual mice with a non-compartmental model using
WinNonLin (Pharsight Inc, Mountain View Calif.). Nominal times and
dose were used with uniform weighing of points. The time points
used (lambda Z ranges) were from 4 days to the end of the study,
although all time points were used for the faster clearing mutants,
P257N and P257L.
[0204] Five antibody PK studies in mFcRn.sup.-/- hFcRn.sup.+ mice
were carried out. FIG. 13a-13b shows serum concentration data for
WT and variant IgG1 (Study 3) and IgG2 (Study 5) antibodies
respectively. Fitted PK parameters from all in vivo PK studies
carried out in mFcRn.sup.-/- hFcRn.sup.+ mice are provided in FIG.
14. PK data include half-life, which represents the beta phase that
characterizes elimination of antibody from serum, Cmax, which
represents the maximal observed serum concentration, AUC, which
represents the area under the concetration time curve, and
clearance, which represents the clearance of antibody from serum.
Also provided for each variant is the calculated fold improvement
or reduction in half-life relative to the IgG1 or IgG2 parent
antibody [Fold half-life =half-life(variant)/half-life (WT)].
[0205] The data show that a number of the engineered Fc variant
antibodies with enhanced FcRn affinity at pH 6.0 extend half-life
in vivo. FIG. 15a shows a plot of the in vivo half-life versus the
fold FcRn binding for the IgG1 antibodies, with select variants
labeled. Results from repeat experiments (circled in the figure)
indicate that data from the in vivo model are reproducible. The
best single variants include 308F and 434S, the best double
variants include 259I/308F, 308F/428L, 308F/4345, and 428L/4345,
and the best triple variant is 259I/308F/428L. There is a general
correlation between affinity for FcRn and in vivo half-life, but it
is not completely predictive. Notably, variants 257L and 257N,
which improved FcRn binding by 3.4 and 3.5-fold respectively,
reduced in vivo half-life by 0.6 and 0.3 respectively. The plot
also highlights again the importance of the amino acid identity of
substitution at a given position--whereas 308F/4345 provided
substantial half-life improvement, 308F/434M was barely better than
WT IgG1.
[0206] FIG. 15b shows a plot of the in vivo half-life versus fold
FcRn binding for the IgG2 variant antibodies with the variants
labeled. When the IgG2 in vivo data were compared with the IgG1 in
vivo data (FIG. 15c), a surprising result was observed. The
variants provided a substantially greater improvement to in vivo
half-life in the context of an IgG2 Fc region than they do an IgG1
Fc region. The longest single variant and double variant half-lives
from all antibodies in all 5 studies were 12.2 and 16.5, provided
by 434S IgG2 and 428L/434S IgG2 respectively. The dramatic
improvement in half-lives for the IgG2 variants relative to IgG1
were despite the fact that fold-improvements by the variants in
IgG2 were comparable or even lower than they were in IgG1 (434S
IgG1 fold =3.8, 434S IgG2 fold =4.9, 428L/434S IgG1 fold=17.3,
428L/434S IgG2 fold=14.8). Thus unexpectedly, the IgG2 antibody may
be the best application for the Fc variants for improving in vivo
half-life in mammals.
Example 6
Variant Immunoadhesins
[0207] The Fc variants of the invention were also evaluated for
their capacity to improve the half-life of immunoadhesins (also
referred to as Fc fusions). Select Fc variants were engineered into
the anti-TNF immunoadhesion etanercept (Enbrel.RTM.). Etanercept is
a fusion of human TNF receptor 2 (TNF RIII) and the Fc region of
human IgG1, and is clinically approved for the treatment of
rheumatoid arthritis, juvenile idiopathic arthritis, ankylosing
spondylitis, psoriatic arthritis, and psoriasis. An IgG2 Fc region
version of this Fc fusion was also constructed, and select Fc
variants were constructed in this context as well. The amino acid
sequences of the anti-TNF immunoadhesins characterized in the
invention are provided in FIG. 16. Genes were constructed using
recursive PCR and subcloned into the pTT5 vector, and Fc variants
were constructed using QuikChange.RTM. mutagenesis methods.
Immunoadhesins were expressed in 293E cells and purified as
described above.
[0208] The binding specificity of the purified immunoadhesins was
confirmed by testing binding to recombinant TNF by Biacore.
Immunoadhesins were captured onto an immobilized Protein A/G
(Pierce) CM5 biosensor chip (Biacore), generated using standard
primary amine coupling. Immunoadhesins were immobilized on the
Protein A/G surface, and recombinant TNF in serial dilutions was
injected over antibody bound surface, followed by a dissociation
phase. After each cycle, the surface was regenerated with buffer.
Data were processed by zeroing time and response before the
injection of receptor and by subtracting from a reference channel
to account for changes due to injections. Kinetic data were fit to
a 1:1 binding model (Langmuir). Equilibrium association constants
(Ka's) obtained from these fits are provided in FIG. 17. The
results show that the variant immunoadhesins retained affinity for
TNF, comparable to commercial enbrel.
[0209] Variant immunoadhesins were tested for binding to human FcRn
at pH 6.0 using Biacore as described above. The results (FIG. 18)
indicate that, similar as in the context of antibodies, the
variants improve binding to FcRn relative to their IgG1 and IgG2
parent immunoadhesin proteins.
[0210] The half-lives of the variant immunoadhesins were tested in
the mFcRn.sup.-/- hFcRn.sup.+ mice as described above. 12 mice per
group were injected at 2 mg/kg of variant and parent IgG1
immunoadhesin. Serum concentration was detected using an ELISA
similar to that described above, except that goat anti-human TNF
RII antibody was used as capture reagent; detection was carried out
with biotinylated anti-human kappa antibody and europium-labeled
streptavidin. FIG. 19 shows serum concentration data for WT IgG1 Fc
and variant Fc immunoadhesins. Fitted PK parameters, as described
above, from the PK study are provided in FIG. 20. Also provided for
each variant is the calculated % increase in half-life, calculated
as 100 times the half-life of variant Fc fusion over that of the WT
IgG1 Fc parent. The results indicate that the variants extend in
vivo half-life in the context of the immunoadhesin.
Example 7
Pharmacokinetic Experiment in Nonhuman Primates
[0211] The PK properties of biologics in non-human primates are
well-established to be predictive of their properties in humans. A
PK study was carried out in cynomolgus monkeys (macaca
fascicularis) in order to evaluate the capacity of the variant
anti-VEGF antibodies to improve serum half-life in non-human
primates.
[0212] In preparation for a PK study in cynomolgus monkeys, binding
of the variant antibodies to cynomolgus (cyno) FcRn (cFcRn) at pH
6.0 was measured. cFcRn was constructed, expressed, and purified as
described above for human FcRn. Binding of variant anti-VEGF
antibodies to cFcRn was measured using Biacore as described above.
The data is provided in FIGS. 21a-21b. The results show that the
variants improve affinity for cyno FcRn similarly as they do for
human FcRn. Dissociation at the higher pH (7.4) was also very rapid
(data not shown), similar to as was observed for binding to human
FcRn. These results are not surprising given the high sequence
homology of the human and cyno receptors (FcRn alpha chain 96%,
beta-2-microglobulin 91%).
[0213] The PK of the variants were studied in vivo in non-human
primates. Male cynomolgus monkeys (macaca fascicularis, also called
crab-eating Macaque) weighing 2.3-5.1 kg were randomized by weight
and divided into 5 groups with 3 monkeys per group. The monkeys
were given a single, 1 hour peripheral vein infusion of 4 mg/kg
antibody. Blood samples (1 ml) were drawn from a separate vein from
5 minutes to 90 days after completion of the infusion, processed to
serum and stored at -70C. Animals were not harmed during these
studies.
[0214] Antibody concentrations were determined using the VEGF
capture method as described above. PK parameters were determined by
fitting the concentrations versus time to a non-compartmental model
as was done in the mouse PK studies. However, time points from day
10 to day 90 were used for PK parameter determinations. The PK
results are plotted in FIG. 22, and the fitted parameters are
provided in FIG. 23. The results show that the variants enhanced
the in vivo half-life of antibody up to 3.2-fold. In the best case
(the 428L/434S variant) half-life was extended from 9.7 days to
31.1 days. The PK results obtained in cynomolgus monkeys are
consistent with those obtained in mFcRn.sup.-/- hFcRn.sup.+ mice,
validating the hFcRn mouse model as a system for assessing the in
vivo PK properties of the variants, and supporting the conclusions
from those studies.
[0215] Whereas particular embodiments of the invention have been
described above for purposes of illustration, it will be
appreciated by those skilled in the art that numerous variations of
the details may be made without departing from the invention as
described in the appended claims. All references cited herein are
incorporated in their entirety.
Sequence CWU 1
1
351107PRTHomo sapiens 1Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe
Pro Pro Ser Asp Glu 1 5 10 15 Gln Leu Lys Ser Gly Thr Ala Ser Val
Val Cys Leu Leu Asn Asn Phe 20 25 30 Tyr Pro Arg Glu Ala Lys Val
Gln Trp Lys Val Asp Asn Ala Leu Gln 35 40 45 Ser Gly Asn Ser Gln
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 50 55 60 Thr Tyr Ser
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 65 70 75 80 Lys
His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 85 90
95 Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 100 105 2330PRTHomo
sapiens 2Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser
Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn
Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val
Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr
Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn
Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115
120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
Cys 130 135 140 Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala
Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg
Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp Trp Leu Asn
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 225 230 235
240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Met His
Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser
Leu Ser Pro Gly Lys 325 330 3326PRTHomo sapiens 3Ala Ser Thr Lys
Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 1 5 10 15 Ser Thr
Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35
40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr
Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly
Thr Gln Thr 65 70 75 80 Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn
Thr Lys Val Asp Lys 85 90 95 Thr Val Glu Arg Lys Cys Cys Val Glu
Cys Pro Pro Cys Pro Ala Pro 100 105 110 Pro Val Ala Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp 115 120 125 Thr Leu Met Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 130 135 140 Val Ser His
Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 145 150 155 160
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 165
170 175 Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp
Trp 180 185 190 Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Gly Leu Pro 195 200 205 Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys
Gly Gln Pro Arg Glu 210 215 220 Pro Gln Val Tyr Thr Leu Pro Pro Ser
Arg Glu Glu Met Thr Lys Asn 225 230 235 240 Gln Val Ser Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 245 250 255 Ala Val Glu Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 260 265 270 Thr Pro
Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 275 280 285
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 290
295 300 Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
Leu 305 310 315 320 Ser Leu Ser Pro Gly Lys 325 4377PRTHomo sapiens
4Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 1
5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp
Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala
Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser
Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser
Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Thr Cys Asn Val Asn His
Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Arg Val Glu Leu Lys
Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro 100 105 110 Arg Cys Pro
Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 115 120 125 Cys
Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys 130 135
140 Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro
145 150 155 160 Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe
Pro Pro Lys 165 170 175 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
Glu Val Thr Cys Val 180 185 190 Val Val Asp Val Ser His Glu Asp Pro
Glu Val Gln Phe Lys Trp Tyr 195 200 205 Val Asp Gly Val Glu Val His
Asn Ala Lys Thr Lys Pro Arg Glu Glu 210 215 220 Gln Tyr Asn Ser Thr
Phe Arg Val Val Ser Val Leu Thr Val Leu His 225 230 235 240 Gln Asp
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 245 250 255
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln 260
265 270 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
Met 275 280 285 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly
Phe Tyr Pro 290 295 300 Ser Asp Ile Ala Val Glu Trp Glu Ser Ser Gly
Gln Pro Glu Asn Asn 305 310 315 320 Tyr Asn Thr Thr Pro Pro Met Leu
Asp Ser Asp Gly Ser Phe Phe Leu 325 330 335 Tyr Ser Lys Leu Thr Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn Ile 340 345 350 Phe Ser Cys Ser
Val Met His Glu Ala Leu His Asn Arg Phe Thr Gln 355 360 365 Lys Ser
Leu Ser Leu Ser Pro Gly Lys 370 375 5327PRTHomo sapiens 5Ala Ser
Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 1 5 10 15
Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20
25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr
Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly
Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser
Leu Gly Thr Lys Thr 65 70 75 80 Tyr Thr Cys Asn Val Asp His Lys Pro
Ser Asn Thr Lys Val Asp Lys 85 90 95 Arg Val Glu Ser Lys Tyr Gly
Pro Pro Cys Pro Ser Cys Pro Ala Pro 100 105 110 Glu Phe Leu Gly Gly
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 115 120 125 Asp Thr Leu
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 130 135 140 Asp
Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 145 150
155 160 Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
Phe 165 170 175 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
His Gln Asp 180 185 190 Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val
Ser Asn Lys Gly Leu 195 200 205 Pro Ser Ser Ile Glu Lys Thr Ile Ser
Lys Ala Lys Gly Gln Pro Arg 210 215 220 Glu Pro Gln Val Tyr Thr Leu
Pro Pro Ser Gln Glu Glu Met Thr Lys 225 230 235 240 Asn Gln Val Ser
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 245 250 255 Ile Ala
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 260 265 270
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 275
280 285 Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe
Ser 290 295 300 Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
Gln Lys Ser 305 310 315 320 Leu Ser Leu Ser Leu Gly Lys 325
6329PRTArtificial SequenceIgG1/2 constant heavy chain
(CH1-hinge-CH2-CH3)) 6Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu
Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu
Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val
Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr
Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90
95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
100 105 110 Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro
Pro Lys 115 120 125 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
Val Thr Cys Val 130 135 140 Val Val Asp Val Ser His Glu Asp Pro Glu
Val Gln Phe Asn Trp Tyr 145 150 155 160 Val Asp Gly Val Glu Val His
Asn Ala Lys Thr Lys Pro Arg Glu Glu 165 170 175 Gln Phe Asn Ser Thr
Phe Arg Val Val Ser Val Leu Thr Val Val His 180 185 190 Gln Asp Trp
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 195 200 205 Gly
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln 210 215
220 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met
225 230 235 240 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly
Phe Tyr Pro 245 250 255 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
Gln Pro Glu Asn Asn 260 265 270 Tyr Lys Thr Thr Pro Pro Met Leu Asp
Ser Asp Gly Ser Phe Phe Leu 275 280 285 Tyr Ser Lys Leu Thr Val Asp
Lys Ser Arg Trp Gln Gln Gly Asn Val 290 295 300 Phe Ser Cys Ser Val
Met His Glu Ala Leu His Asn His Tyr Thr Gln 305 310 315 320 Lys Ser
Leu Ser Leu Ser Pro Gly Lys 325 7330PRTArtificial SequenceIgG1
259I/308F 7Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser
Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn
Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val
Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr
Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn
Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115
120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Ile Thr
Cys 130 135 140 Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala
Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg
Val Val Ser Val Leu Thr Phe Leu 180 185 190 His Gln Asp Trp Leu Asn
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 225 230 235
240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Met His
Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser
Leu Ser Pro Gly Lys 325 330 8330PRTArtificial SequenceIgG1
434S/428L 8Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser
Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn
Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val
Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr
Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn
Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115
120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
Cys 130 135 140
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 145
150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro
Arg Glu 165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
Leu Thr Val Leu 180 185 190 His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro Ala Pro Ile Glu
Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro Arg Glu Pro Gln
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 225 230 235 240 Met Thr Lys
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 245 250 255 Pro
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 260 265
270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln
Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Leu His Glu Ala Leu His
Ser His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser Leu Ser Pro Gly
Lys 325 330 9326PRTArtificial SequenceIgG2 434S 9Ala Ser Thr Lys
Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 1 5 10 15 Ser Thr
Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35
40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr
Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly
Thr Gln Thr 65 70 75 80 Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn
Thr Lys Val Asp Lys 85 90 95 Thr Val Glu Arg Lys Cys Cys Val Glu
Cys Pro Pro Cys Pro Ala Pro 100 105 110 Pro Val Ala Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp 115 120 125 Thr Leu Met Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 130 135 140 Val Ser His
Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 145 150 155 160
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 165
170 175 Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp
Trp 180 185 190 Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Gly Leu Pro 195 200 205 Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys
Gly Gln Pro Arg Glu 210 215 220 Pro Gln Val Tyr Thr Leu Pro Pro Ser
Arg Glu Glu Met Thr Lys Asn 225 230 235 240 Gln Val Ser Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 245 250 255 Ala Val Glu Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 260 265 270 Thr Pro
Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 275 280 285
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 290
295 300 Ser Val Met His Glu Ala Leu His Ser His Tyr Thr Gln Lys Ser
Leu 305 310 315 320 Ser Leu Ser Pro Gly Lys 325 10326PRTArtificial
SequenceIgG2 434S/428L 10Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
Leu Ala Pro Cys Ser Arg 1 5 10 15 Ser Thr Ser Glu Ser Thr Ala Ala
Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr
Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr
Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser
Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 65 70 75 80
Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 85
90 95 Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala
Pro 100 105 110 Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
Pro Lys Asp 115 120 125 Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
Cys Val Val Val Asp 130 135 140 Val Ser His Glu Asp Pro Glu Val Gln
Phe Asn Trp Tyr Val Asp Gly 145 150 155 160 Val Glu Val His Asn Ala
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 165 170 175 Ser Thr Phe Arg
Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 180 185 190 Leu Asn
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 195 200 205
Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 210
215 220 Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys
Asn 225 230 235 240 Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
Pro Ser Asp Ile 245 250 255 Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
Glu Asn Asn Tyr Lys Thr 260 265 270 Thr Pro Pro Met Leu Asp Ser Asp
Gly Ser Phe Phe Leu Tyr Ser Lys 275 280 285 Leu Thr Val Asp Lys Ser
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 290 295 300 Ser Val Leu His
Glu Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu 305 310 315 320 Ser
Leu Ser Pro Gly Lys 325 11329PRTArtificial SequenceIgG1/2 434S
11Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1
5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp
Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala
Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser
Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser
Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His
Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys
Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro
Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 115 120 125 Pro
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 130 135
140 Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr
145 150 155 160 Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro
Arg Glu Glu 165 170 175 Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val
Leu Thr Val Val His 180 185 190 Gln Asp Trp Leu Asn Gly Lys Glu Tyr
Lys Cys Lys Val Ser Asn Lys 195 200 205 Gly Leu Pro Ala Pro Ile Glu
Lys Thr Ile Ser Lys Thr Lys Gly Gln 210 215 220 Pro Arg Glu Pro Gln
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 225 230 235 240 Thr Lys
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 245 250 255
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 260
265 270 Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe
Leu 275 280 285 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln
Gly Asn Val 290 295 300 Phe Ser Cys Ser Val Met His Glu Ala Leu His
Ser His Tyr Thr Gln 305 310 315 320 Lys Ser Leu Ser Leu Ser Pro Gly
Lys 325 12329PRTArtificial SequenceIgG1/2 434S/428L 12Ala Ser Thr
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1 5 10 15 Ser
Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25
30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu
Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His Lys Pro Ser
Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys Ser Cys Asp
Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro Pro Val Ala
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 115 120 125 Pro Lys Asp Thr
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 130 135 140 Val Val
Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 145 150 155
160 Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
165 170 175 Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val
Val His 180 185 190 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
Val Ser Asn Lys 195 200 205 Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile
Ser Lys Thr Lys Gly Gln 210 215 220 Pro Arg Glu Pro Gln Val Tyr Thr
Leu Pro Pro Ser Arg Glu Glu Met 225 230 235 240 Thr Lys Asn Gln Val
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 245 250 255 Ser Asp Ile
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 260 265 270 Tyr
Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu 275 280
285 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
290 295 300 Phe Ser Cys Ser Val Leu His Glu Ala Leu His Ser His Tyr
Thr Gln 305 310 315 320 Lys Ser Leu Ser Leu Ser Pro Gly Lys 325
13123PRTArtificial SequenceAnti-VEGF VH 13Glu Val Gln Leu Val Glu
Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu
Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 20 25 30 Gly Met
Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45
Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 50
55 60 Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala
Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val
Tyr Tyr Cys 85 90 95 Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His
Trp Tyr Phe Asp Val 100 105 110 Trp Gly Gln Gly Thr Leu Val Thr Val
Ser Ser 115 120 14107PRTArtificial SequenceAnti-VEGF VL 14Asp Ile
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15
Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 20
25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu
Ile 35 40 45 Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg
Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln
Gln Tyr Ser Thr Val Pro Trp 85 90 95 Thr Phe Gly Gln Gly Thr Lys
Val Glu Ile Lys 100 105 15121PRTArtificial SequenceAnti-TNF VH
15Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 1
5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp
Tyr 20 25 30 Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
Glu Trp Val 35 40 45 Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp
Tyr Ala Asp Ser Val 50 55 60 Glu Gly Arg Phe Thr Ile Ser Arg Asp
Asn Ala Lys Asn Ser Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg
Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Val Ser Tyr
Leu Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly 100 105 110 Gln Gly Thr
Leu Val Thr Val Ser Ser 115 120 16107PRTArtificial SequenceAnti-TNF
VL 16Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile
Arg Asn Tyr 20 25 30 Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala
Pro Lys Leu Leu Ile 35 40 45 Tyr Ala Ala Ser Thr Leu Gln Ser Gly
Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe
Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Val Ala Thr
Tyr Tyr Cys Gln Arg Tyr Asn Arg Ala Pro Tyr 85 90 95 Thr Phe Gly
Gln Gly Thr Lys Val Glu Ile Lys 100 105 17116PRTArtificial
SequenceAnti-CD25 17Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys
Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly
Tyr Thr Phe Thr Ser Tyr 20 25 30 Arg Met His Trp Val Arg Gln Ala
Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Trp Ile Asn Pro Ser
Thr Gly Tyr Thr Glu Tyr Asn Gln Lys Phe 50 55 60 Gln Gly Arg Val
Thr Ile Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 65 70 75 80 Met Glu
Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 85 90 95
Ala Arg Gly Gly Gly Val Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 100
105 110 Thr Val Ser Ser 115 18106PRTArtificial SequenceAnti-CD25 VL
18Gln Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 1
5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Ser Ser Ile Ser Tyr
Met 20 25 30 His Trp Phe Gln Gln Lys Pro Gly Gln Ser Pro Gln Leu
Leu Ile Tyr 35 40 45 Thr Thr Ser Asn Leu Ala Ser Gly Val Pro Ala
Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp Tyr Thr Leu Thr
Ile Ser Ser Leu Gln Pro Glu 65 70 75 80 Asp Phe Ala Val Tyr Tyr Cys
His Gln Arg Ser Thr Tyr Pro Leu Thr 85 90 95 Phe Gly Ser Gly Thr
Lys Leu Glu Ile Lys 100 105 19119PRTArtificial SequenceAnti-EGFR VH
19Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 1
5 10 15 Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Asn
Tyr 20 25 30 Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
Glu Trp Met 35 40 45 Gly Ile Ile Trp Ser Gly Gly Ser Thr Asp Tyr
Ser Thr Ser Leu Lys 50 55 60 Ser Arg Leu Thr Ile Ser Lys Asp
Thr Ser Lys Ser Gln Val Val Leu 65 70 75 80 Thr Met Thr Asn Met Asp
Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala 85 90 95 Arg Ala Leu Thr
Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp Gly Gln Gly 100 105 110 Thr Leu
Val Thr Val Ser Ser 115 20107PRTArtificial SequenceAnti-EGFR VL
20Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1
5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser
Asn 20 25 30 Leu His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys
Leu Leu Ile 35 40 45 Lys Tyr Ala Ser Glu Ser Ile Ser Gly Val Pro
Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu
Thr Ile Ser Ser Leu Gln Ala 65 70 75 80 Glu Asp Val Ala Val Tyr Tyr
Cys Gln Gln Asn Asn Asn Trp Pro Thr 85 90 95 Thr Phe Gly Gln Gly
Thr Lys Leu Glu Ile Lys 100 105 21121PRTArtificial SequenceAnti-IgE
VH 21Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Tyr Ser Ile
Thr Ser Gly 20 25 30 Tyr Ser Trp Asn Trp Ile Arg Gln Ala Pro Gly
Lys Gly Leu Glu Trp 35 40 45 Val Ala Ser Ile Thr Tyr Asp Gly Ser
Thr Asn Tyr Asn Pro Ser Val 50 55 60 Lys Gly Arg Ile Thr Ile Ser
Arg Asp Asp Ser Lys Asn Thr Phe Tyr 65 70 75 80 Leu Gln Met Asn Ser
Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Gly
Ser His Tyr Phe Gly His Trp His Phe Ala Val Trp Gly 100 105 110 Gln
Gly Thr Leu Val Thr Val Ser Ser 115 120 22111PRTArtificial
SequenceAnti-IgE VL 22Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu
Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala
Ser Gln Ser Val Asp Tyr Asp 20 25 30 Gly Asp Ser Tyr Met Asn Trp
Tyr Gln Gln Lys Pro Gly Lys Ala Pro 35 40 45 Lys Leu Leu Ile Tyr
Ala Ala Ser Tyr Leu Glu Ser Gly Val Pro Ser 50 55 60 Arg Phe Ser
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 65 70 75 80 Ser
Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser His 85 90
95 Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100
105 110 23214PRTArtificial SequenceAnti-TNF light chain 23Asp Ile
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Tyr 20
25 30 Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
Ile 35 40 45 Tyr Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg
Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Val Ala Thr Tyr Tyr Cys Gln
Arg Tyr Asn Arg Ala Pro Tyr 85 90 95 Thr Phe Gly Gln Gly Thr Lys
Val Glu Ile Lys Arg Thr Val Ala Ala 100 105 110 Pro Ser Val Phe Ile
Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 115 120 125 Thr Ala Ser
Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 130 135 140 Lys
Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 145 150
155 160 Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu
Ser 165 170 175 Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
Lys Val Tyr 180 185 190 Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser
Pro Val Thr Lys Ser 195 200 205 Phe Asn Arg Gly Glu Cys 210
24451PRTArtificial SequenceAnti-TNF heavy chain IgG1 259I/308F
24Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 1
5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp
Tyr 20 25 30 Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
Glu Trp Val 35 40 45 Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp
Tyr Ala Asp Ser Val 50 55 60 Glu Gly Arg Phe Thr Ile Ser Arg Asp
Asn Ala Lys Asn Ser Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg
Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Val Ser Tyr
Leu Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly 100 105 110 Gln Gly Thr
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115 120 125 Val
Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 130 135
140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val
145 150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr
Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser
Ser Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly Thr Gln Thr
Tyr Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn Thr Lys Val
Asp Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys Thr His Thr
Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 225 230 235 240 Gly Pro
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 245 250 255
Ile Ser Arg Thr Pro Glu Ile Thr Cys Val Val Val Asp Val Ser His 260
265 270 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
Val 275 280 285 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
Ser Thr Tyr 290 295 300 Arg Val Val Ser Val Leu Thr Phe Leu His Gln
Asp Trp Leu Asn Gly 305 310 315 320 Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys Ala Leu Pro Ala Pro Ile 325 330 335 Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 340 345 350 Tyr Thr Leu Pro
Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 355 360 365 Leu Thr
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 370 375 380
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 385
390 395 400 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
Thr Val 405 410 415 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser
Cys Ser Val Met 420 425 430 His Glu Ala Leu His Asn His Tyr Thr Gln
Lys Ser Leu Ser Leu Ser 435 440 445 Pro Gly Lys 450
25451PRTArtificial SequenceAnti-TNF heavy chain IgG1 434S/428L
25Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 1
5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp
Tyr 20 25 30 Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
Glu Trp Val 35 40 45 Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp
Tyr Ala Asp Ser Val 50 55 60 Glu Gly Arg Phe Thr Ile Ser Arg Asp
Asn Ala Lys Asn Ser Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg
Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Val Ser Tyr
Leu Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly 100 105 110 Gln Gly Thr
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115 120 125 Val
Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 130 135
140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val
145 150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr
Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser
Ser Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly Thr Gln Thr
Tyr Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn Thr Lys Val
Asp Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys Thr His Thr
Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 225 230 235 240 Gly Pro
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 245 250 255
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 260
265 270 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
Val 275 280 285 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
Ser Thr Tyr 290 295 300 Arg Val Val Ser Val Leu Thr Val Leu His Gln
Asp Trp Leu Asn Gly 305 310 315 320 Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys Ala Leu Pro Ala Pro Ile 325 330 335 Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 340 345 350 Tyr Thr Leu Pro
Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 355 360 365 Leu Thr
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 370 375 380
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 385
390 395 400 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
Thr Val 405 410 415 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser
Cys Ser Val Leu 420 425 430 His Glu Ala Leu His Ser His Tyr Thr Gln
Lys Ser Leu Ser Leu Ser 435 440 445 Pro Gly Lys 450
26447PRTArtificial SequenceAnti-TNF heavy chain IgG2 434S 26Glu Val
Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 20
25 30 Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
Val 35 40 45 Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala
Asp Ser Val 50 55 60 Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala
Lys Asn Ser Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu
Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Val Ser Tyr Leu Ser
Thr Ala Ser Ser Leu Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu Val
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115 120 125 Val Phe Pro
Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala 130 135 140 Ala
Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 145 150
155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro
Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val
Val Thr Val 180 185 190 Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr Thr
Cys Asn Val Asp His 195 200 205 Lys Pro Ser Asn Thr Lys Val Asp Lys
Thr Val Glu Arg Lys Cys Cys 210 215 220 Val Glu Cys Pro Pro Cys Pro
Ala Pro Pro Val Ala Gly Pro Ser Val 225 230 235 240 Phe Leu Phe Pro
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 245 250 255 Pro Glu
Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 260 265 270
Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 275
280 285 Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
Ser 290 295 300 Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr Lys 305 310 315 320 Cys Lys Val Ser Asn Lys Gly Leu Pro Ala
Pro Ile Glu Lys Thr Ile 325 330 335 Ser Lys Thr Lys Gly Gln Pro Arg
Glu Pro Gln Val Tyr Thr Leu Pro 340 345 350 Pro Ser Arg Glu Glu Met
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 355 360 365 Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 370 375 380 Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser 385 390 395
400 Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg
405 410 415 Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
Ala Leu 420 425 430 His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 435 440 445 27447PRTArtificial SequenceAnti-TNF heavy
chain IgG2 434S/428L 27Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu
Val Gln Pro Gly Arg 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser
Gly Phe Thr Phe Asp Asp Tyr 20 25 30 Ala Met His Trp Val Arg Gln
Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ser Ala Ile Thr Trp
Asn Ser Gly His Ile Asp Tyr Ala Asp Ser Val 50 55 60 Glu Gly Arg
Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 65 70 75 80 Leu
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90
95 Ala Lys Val Ser Tyr Leu Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly
100 105 110 Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly
Pro Ser 115 120 125 Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser
Glu Ser Thr Ala 130 135 140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe
Pro Glu Pro Val Thr Val 145 150 155 160 Ser Trp Asn Ser Gly Ala Leu
Thr Ser Gly Val His Thr Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser
Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 180 185 190 Pro Ser Ser
Asn Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His 195 200 205 Lys
Pro Ser Asn Thr Lys Val Asp Lys Thr Val Glu Arg Lys Cys Cys 210 215
220 Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val
225 230 235 240 Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
Ser Arg Thr 245 250 255 Pro Glu Val Thr Cys Val Val Val Asp Val Ser
His Glu Asp Pro Glu 260 265 270 Val Gln Phe Asn Trp Tyr Val Asp Gly
Val Glu Val His Asn Ala Lys 275 280 285 Thr Lys Pro Arg Glu Glu
Gln
Phe Asn Ser Thr Phe Arg Val Val Ser 290 295 300 Val Leu Thr Val Val
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 305 310 315 320 Cys Lys
Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile 325 330 335
Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 340
345 350 Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
Leu 355 360 365 Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
Glu Ser Asn 370 375 380 Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
Pro Met Leu Asp Ser 385 390 395 400 Asp Gly Ser Phe Phe Leu Tyr Ser
Lys Leu Thr Val Asp Lys Ser Arg 405 410 415 Trp Gln Gln Gly Asn Val
Phe Ser Cys Ser Val Leu His Glu Ala Leu 420 425 430 His Ser His Tyr
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 435 440 445
28450PRTArtificial SequenceAnti-TNF heavy chain IgG1/2 434S 28Glu
Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 1 5 10
15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr
20 25 30 Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu
Trp Val 35 40 45 Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr
Ala Asp Ser Val 50 55 60 Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn
Ala Lys Asn Ser Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala
Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Val Ser Tyr Leu
Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu
Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115 120 125 Val Phe
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 130 135 140
Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 145
150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser
Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr
Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn Thr Lys Val Asp
Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys Thr His Thr Cys
Pro Pro Cys Pro Ala Pro Pro Val Ala Gly 225 230 235 240 Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 245 250 255 Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 260 265
270 Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
275 280 285 Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr
Phe Arg 290 295 300 Val Val Ser Val Leu Thr Val Val His Gln Asp Trp
Leu Asn Gly Lys 305 310 315 320 Glu Tyr Lys Cys Lys Val Ser Asn Lys
Gly Leu Pro Ala Pro Ile Glu 325 330 335 Lys Thr Ile Ser Lys Thr Lys
Gly Gln Pro Arg Glu Pro Gln Val Tyr 340 345 350 Thr Leu Pro Pro Ser
Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 355 360 365 Thr Cys Leu
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 370 375 380 Glu
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met 385 390
395 400 Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
Asp 405 410 415 Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met His 420 425 430 Glu Ala Leu His Ser His Tyr Thr Gln Lys Ser
Leu Ser Leu Ser Pro 435 440 445 Gly Lys 450 29450PRTArtificial
SequenceAnti-TNF heavy chain IgG1/2 434S/428L 29Glu Val Gln Leu Val
Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 1 5 10 15 Ser Leu Arg
Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 20 25 30 Ala
Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40
45 Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala Asp Ser Val
50 55 60 Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser
Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala
Val Tyr Tyr Cys 85 90 95 Ala Lys Val Ser Tyr Leu Ser Thr Ala Ser
Ser Leu Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu Val Thr Val Ser
Ser Ala Ser Thr Lys Gly Pro Ser 115 120 125 Val Phe Pro Leu Ala Pro
Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 130 135 140 Ala Leu Gly Cys
Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 145 150 155 160 Ser
Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 165 170
175 Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val
180 185 190 Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val
Asn His 195 200 205 Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu
Pro Lys Ser Cys 210 215 220 Asp Lys Thr His Thr Cys Pro Pro Cys Pro
Ala Pro Pro Val Ala Gly 225 230 235 240 Pro Ser Val Phe Leu Phe Pro
Pro Lys Pro Lys Asp Thr Leu Met Ile 245 250 255 Ser Arg Thr Pro Glu
Val Thr Cys Val Val Val Asp Val Ser His Glu 260 265 270 Asp Pro Glu
Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 275 280 285 Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg 290 295
300 Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys
305 310 315 320 Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala
Pro Ile Glu 325 330 335 Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg
Glu Pro Gln Val Tyr 340 345 350 Thr Leu Pro Pro Ser Arg Glu Glu Met
Thr Lys Asn Gln Val Ser Leu 355 360 365 Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp 370 375 380 Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met 385 390 395 400 Leu Asp
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 405 410 415
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His 420
425 430 Glu Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro 435 440 445 Gly Lys 450 30489PRTArtificial SequenceAnti-TNF Fc
fusion with IgG1 Fc 30Met Ala Pro Val Ala Val Trp Ala Ala Leu Ala
Val Gly Leu Glu Leu 1 5 10 15 Trp Ala Ala Ala His Ala Leu Pro Ala
Gln Val Ala Phe Thr Pro Tyr 20 25 30 Ala Pro Glu Pro Gly Ser Thr
Cys Arg Leu Arg Glu Tyr Tyr Asp Gln 35 40 45 Thr Ala Gln Met Cys
Cys Ser Lys Cys Ser Pro Gly Gln His Ala Lys 50 55 60 Val Phe Cys
Thr Lys Thr Ser Asp Thr Val Cys Asp Ser Cys Glu Asp 65 70 75 80 Ser
Thr Tyr Thr Gln Leu Trp Asn Trp Val Pro Glu Cys Leu Ser Cys 85 90
95 Gly Ser Arg Cys Ser Ser Asp Gln Val Glu Thr Gln Ala Cys Thr Arg
100 105 110 Glu Gln Asn Arg Ile Cys Thr Cys Arg Pro Gly Trp Tyr Cys
Ala Leu 115 120 125 Ser Lys Gln Glu Gly Cys Arg Leu Cys Ala Pro Leu
Arg Lys Cys Arg 130 135 140 Pro Gly Phe Gly Val Ala Arg Pro Gly Thr
Glu Thr Ser Asp Val Val 145 150 155 160 Cys Lys Pro Cys Ala Pro Gly
Thr Phe Ser Asn Thr Thr Ser Ser Thr 165 170 175 Asp Ile Cys Arg Pro
His Gln Ile Cys Asn Val Val Ala Ile Pro Gly 180 185 190 Asn Ala Ser
Met Asp Ala Val Cys Thr Ser Thr Ser Pro Thr Arg Ser 195 200 205 Met
Ala Pro Gly Ala Val His Leu Pro Gln Pro Val Ser Thr Arg Ser 210 215
220 Gln His Thr Gln Pro Thr Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser
225 230 235 240 Phe Leu Leu Pro Met Gly Pro Ser Pro Pro Ala Glu Gly
Ser Thr Gly 245 250 255 Asp Glu Pro Lys Ser Cys Asp Lys Thr His Thr
Cys Pro Pro Cys Pro 260 265 270 Ala Pro Glu Leu Leu Gly Gly Pro Ser
Val Phe Leu Phe Pro Pro Lys 275 280 285 Pro Lys Asp Thr Leu Met Ile
Ser Arg Thr Pro Glu Val Thr Cys Val 290 295 300 Val Val Asp Val Ser
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 305 310 315 320 Val Asp
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 325 330 335
Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 340
345 350 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
Lys 355 360 365 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala
Lys Gly Gln 370 375 380 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
Ser Arg Glu Glu Met 385 390 395 400 Thr Lys Asn Gln Val Ser Leu Thr
Cys Leu Val Lys Gly Phe Tyr Pro 405 410 415 Ser Asp Ile Ala Val Glu
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 420 425 430 Tyr Lys Thr Thr
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 435 440 445 Tyr Ser
Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 450 455 460
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 465
470 475 480 Lys Ser Leu Ser Leu Ser Pro Gly Lys 485
31488PRTArtificial SequenceAnti-TNF Fc fusion with IgG2 Fc 31Met
Ala Pro Val Ala Val Trp Ala Ala Leu Ala Val Gly Leu Glu Leu 1 5 10
15 Trp Ala Ala Ala His Ala Leu Pro Ala Gln Val Ala Phe Thr Pro Tyr
20 25 30 Ala Pro Glu Pro Gly Ser Thr Cys Arg Leu Arg Glu Tyr Tyr
Asp Gln 35 40 45 Thr Ala Gln Met Cys Cys Ser Lys Cys Ser Pro Gly
Gln His Ala Lys 50 55 60 Val Phe Cys Thr Lys Thr Ser Asp Thr Val
Cys Asp Ser Cys Glu Asp 65 70 75 80 Ser Thr Tyr Thr Gln Leu Trp Asn
Trp Val Pro Glu Cys Leu Ser Cys 85 90 95 Gly Ser Arg Cys Ser Ser
Asp Gln Val Glu Thr Gln Ala Cys Thr Arg 100 105 110 Glu Gln Asn Arg
Ile Cys Thr Cys Arg Pro Gly Trp Tyr Cys Ala Leu 115 120 125 Ser Lys
Gln Glu Gly Cys Arg Leu Cys Ala Pro Leu Arg Lys Cys Arg 130 135 140
Pro Gly Phe Gly Val Ala Arg Pro Gly Thr Glu Thr Ser Asp Val Val 145
150 155 160 Cys Lys Pro Cys Ala Pro Gly Thr Phe Ser Asn Thr Thr Ser
Ser Thr 165 170 175 Asp Ile Cys Arg Pro His Gln Ile Cys Asn Val Val
Ala Ile Pro Gly 180 185 190 Asn Ala Ser Met Asp Ala Val Cys Thr Ser
Thr Ser Pro Thr Arg Ser 195 200 205 Met Ala Pro Gly Ala Val His Leu
Pro Gln Pro Val Ser Thr Arg Ser 210 215 220 Gln His Thr Gln Pro Thr
Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser 225 230 235 240 Phe Leu Leu
Pro Met Gly Pro Ser Pro Pro Ala Glu Gly Ser Thr Gly 245 250 255 Asp
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 260 265
270 Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro
275 280 285 Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
Val Val 290 295 300 Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe
Asn Trp Tyr Val 305 310 315 320 Asp Gly Val Glu Val His Asn Ala Lys
Thr Lys Pro Arg Glu Glu Gln 325 330 335 Phe Asn Ser Thr Phe Arg Val
Val Ser Val Leu Thr Val Val His Gln 340 345 350 Asp Trp Leu Asn Gly
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 355 360 365 Leu Pro Ala
Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro 370 375 380 Arg
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 385 390
395 400 Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
Ser 405 410 415 Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
Asn Asn Tyr 420 425 430 Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly
Ser Phe Phe Leu Tyr 435 440 445 Ser Lys Leu Thr Val Asp Lys Ser Arg
Trp Gln Gln Gly Asn Val Phe 450 455 460 Ser Cys Ser Val Met His Glu
Ala Leu His Asn His Tyr Thr Gln Lys 465 470 475 480 Ser Leu Ser Leu
Ser Pro Gly Lys 485 32489PRTArtificial SequenceAnti-TNF Fc fusion
with IgG1 259I/308F Fc 32Met Ala Pro Val Ala Val Trp Ala Ala Leu
Ala Val Gly Leu Glu Leu 1 5 10 15 Trp Ala Ala Ala His Ala Leu Pro
Ala Gln Val Ala Phe Thr Pro Tyr 20 25 30 Ala Pro Glu Pro Gly Ser
Thr Cys Arg Leu Arg Glu Tyr Tyr Asp Gln 35 40 45 Thr Ala Gln Met
Cys Cys Ser Lys Cys Ser Pro Gly Gln His Ala Lys 50 55 60 Val Phe
Cys Thr Lys Thr Ser Asp Thr Val Cys Asp Ser Cys Glu Asp 65 70 75 80
Ser Thr Tyr Thr Gln Leu Trp Asn Trp Val Pro Glu Cys Leu Ser Cys 85
90 95 Gly Ser Arg Cys Ser Ser Asp Gln Val Glu Thr Gln Ala Cys Thr
Arg 100 105 110 Glu Gln Asn Arg Ile Cys Thr Cys Arg Pro Gly Trp Tyr
Cys Ala Leu 115 120 125 Ser Lys Gln Glu Gly Cys Arg Leu Cys Ala Pro
Leu Arg Lys Cys Arg 130 135 140 Pro Gly Phe Gly Val Ala Arg Pro Gly
Thr Glu Thr Ser Asp Val Val 145 150 155 160 Cys Lys Pro Cys Ala Pro
Gly Thr Phe Ser Asn Thr Thr Ser Ser Thr 165 170 175 Asp Ile Cys Arg
Pro His Gln Ile Cys Asn Val Val Ala Ile Pro Gly 180 185 190 Asn Ala
Ser Met Asp Ala Val Cys Thr Ser Thr Ser Pro Thr Arg Ser 195 200 205
Met Ala Pro Gly Ala Val His Leu Pro Gln Pro Val Ser Thr Arg Ser 210
215 220 Gln His Thr Gln Pro Thr Pro Glu Pro Ser Thr Ala
Pro Ser Thr Ser 225 230 235 240 Phe Leu Leu Pro Met Gly Pro Ser Pro
Pro Ala Glu Gly Ser Thr Gly 245 250 255 Asp Glu Pro Lys Ser Cys Asp
Lys Thr His Thr Cys Pro Pro Cys Pro 260 265 270 Ala Pro Glu Leu Leu
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 275 280 285 Pro Lys Asp
Thr Leu Met Ile Ser Arg Thr Pro Glu Ile Thr Cys Val 290 295 300 Val
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 305 310
315 320 Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
Glu 325 330 335 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr
Phe Leu His 340 345 350 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
Lys Val Ser Asn Lys 355 360 365 Ala Leu Pro Ala Pro Ile Glu Lys Thr
Ile Ser Lys Ala Lys Gly Gln 370 375 380 Pro Arg Glu Pro Gln Val Tyr
Thr Leu Pro Pro Ser Arg Glu Glu Met 385 390 395 400 Thr Lys Asn Gln
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 405 410 415 Ser Asp
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 420 425 430
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 435
440 445 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
Val 450 455 460 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His
Tyr Thr Gln 465 470 475 480 Lys Ser Leu Ser Leu Ser Pro Gly Lys 485
33489PRTArtificial SequenceAnti-TNF Fc fusion with IgG1 428L/434S
Fc 33Met Ala Pro Val Ala Val Trp Ala Ala Leu Ala Val Gly Leu Glu
Leu 1 5 10 15 Trp Ala Ala Ala His Ala Leu Pro Ala Gln Val Ala Phe
Thr Pro Tyr 20 25 30 Ala Pro Glu Pro Gly Ser Thr Cys Arg Leu Arg
Glu Tyr Tyr Asp Gln 35 40 45 Thr Ala Gln Met Cys Cys Ser Lys Cys
Ser Pro Gly Gln His Ala Lys 50 55 60 Val Phe Cys Thr Lys Thr Ser
Asp Thr Val Cys Asp Ser Cys Glu Asp 65 70 75 80 Ser Thr Tyr Thr Gln
Leu Trp Asn Trp Val Pro Glu Cys Leu Ser Cys 85 90 95 Gly Ser Arg
Cys Ser Ser Asp Gln Val Glu Thr Gln Ala Cys Thr Arg 100 105 110 Glu
Gln Asn Arg Ile Cys Thr Cys Arg Pro Gly Trp Tyr Cys Ala Leu 115 120
125 Ser Lys Gln Glu Gly Cys Arg Leu Cys Ala Pro Leu Arg Lys Cys Arg
130 135 140 Pro Gly Phe Gly Val Ala Arg Pro Gly Thr Glu Thr Ser Asp
Val Val 145 150 155 160 Cys Lys Pro Cys Ala Pro Gly Thr Phe Ser Asn
Thr Thr Ser Ser Thr 165 170 175 Asp Ile Cys Arg Pro His Gln Ile Cys
Asn Val Val Ala Ile Pro Gly 180 185 190 Asn Ala Ser Met Asp Ala Val
Cys Thr Ser Thr Ser Pro Thr Arg Ser 195 200 205 Met Ala Pro Gly Ala
Val His Leu Pro Gln Pro Val Ser Thr Arg Ser 210 215 220 Gln His Thr
Gln Pro Thr Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser 225 230 235 240
Phe Leu Leu Pro Met Gly Pro Ser Pro Pro Ala Glu Gly Ser Thr Gly 245
250 255 Asp Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
Pro 260 265 270 Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe
Pro Pro Lys 275 280 285 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
Glu Val Thr Cys Val 290 295 300 Val Val Asp Val Ser His Glu Asp Pro
Glu Val Lys Phe Asn Trp Tyr 305 310 315 320 Val Asp Gly Val Glu Val
His Asn Ala Lys Thr Lys Pro Arg Glu Glu 325 330 335 Gln Tyr Asn Ser
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 340 345 350 Gln Asp
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 355 360 365
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 370
375 380 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
Met 385 390 395 400 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
Gly Phe Tyr Pro 405 410 415 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
Gly Gln Pro Glu Asn Asn 420 425 430 Tyr Lys Thr Thr Pro Pro Val Leu
Asp Ser Asp Gly Ser Phe Phe Leu 435 440 445 Tyr Ser Lys Leu Thr Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 450 455 460 Phe Ser Cys Ser
Val Leu His Glu Ala Leu His Ser His Tyr Thr Gln 465 470 475 480 Lys
Ser Leu Ser Leu Ser Pro Gly Lys 485 34488PRTArtificial
SequenceAnti-TNF Fc fusion with IgG2 434S Fc 34Met Ala Pro Val Ala
Val Trp Ala Ala Leu Ala Val Gly Leu Glu Leu 1 5 10 15 Trp Ala Ala
Ala His Ala Leu Pro Ala Gln Val Ala Phe Thr Pro Tyr 20 25 30 Ala
Pro Glu Pro Gly Ser Thr Cys Arg Leu Arg Glu Tyr Tyr Asp Gln 35 40
45 Thr Ala Gln Met Cys Cys Ser Lys Cys Ser Pro Gly Gln His Ala Lys
50 55 60 Val Phe Cys Thr Lys Thr Ser Asp Thr Val Cys Asp Ser Cys
Glu Asp 65 70 75 80 Ser Thr Tyr Thr Gln Leu Trp Asn Trp Val Pro Glu
Cys Leu Ser Cys 85 90 95 Gly Ser Arg Cys Ser Ser Asp Gln Val Glu
Thr Gln Ala Cys Thr Arg 100 105 110 Glu Gln Asn Arg Ile Cys Thr Cys
Arg Pro Gly Trp Tyr Cys Ala Leu 115 120 125 Ser Lys Gln Glu Gly Cys
Arg Leu Cys Ala Pro Leu Arg Lys Cys Arg 130 135 140 Pro Gly Phe Gly
Val Ala Arg Pro Gly Thr Glu Thr Ser Asp Val Val 145 150 155 160 Cys
Lys Pro Cys Ala Pro Gly Thr Phe Ser Asn Thr Thr Ser Ser Thr 165 170
175 Asp Ile Cys Arg Pro His Gln Ile Cys Asn Val Val Ala Ile Pro Gly
180 185 190 Asn Ala Ser Met Asp Ala Val Cys Thr Ser Thr Ser Pro Thr
Arg Ser 195 200 205 Met Ala Pro Gly Ala Val His Leu Pro Gln Pro Val
Ser Thr Arg Ser 210 215 220 Gln His Thr Gln Pro Thr Pro Glu Pro Ser
Thr Ala Pro Ser Thr Ser 225 230 235 240 Phe Leu Leu Pro Met Gly Pro
Ser Pro Pro Ala Glu Gly Ser Thr Gly 245 250 255 Asp Glu Pro Lys Ser
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 260 265 270 Ala Pro Pro
Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 275 280 285 Lys
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 290 295
300 Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val
305 310 315 320 Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
Glu Glu Gln 325 330 335 Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu
Thr Val Val His Gln 340 345 350 Asp Trp Leu Asn Gly Lys Glu Tyr Lys
Cys Lys Val Ser Asn Lys Gly 355 360 365 Leu Pro Ala Pro Ile Glu Lys
Thr Ile Ser Lys Thr Lys Gly Gln Pro 370 375 380 Arg Glu Pro Gln Val
Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 385 390 395 400 Lys Asn
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 405 410 415
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 420
425 430 Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu
Tyr 435 440 445 Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
Asn Val Phe 450 455 460 Ser Cys Ser Val Met His Glu Ala Leu His Ser
His Tyr Thr Gln Lys 465 470 475 480 Ser Leu Ser Leu Ser Pro Gly Lys
485 35488PRTArtificial SequenceAnti-TNF Fc fusion with IgG2
428L/434S Fc 35Met Ala Pro Val Ala Val Trp Ala Ala Leu Ala Val Gly
Leu Glu Leu 1 5 10 15 Trp Ala Ala Ala His Ala Leu Pro Ala Gln Val
Ala Phe Thr Pro Tyr 20 25 30 Ala Pro Glu Pro Gly Ser Thr Cys Arg
Leu Arg Glu Tyr Tyr Asp Gln 35 40 45 Thr Ala Gln Met Cys Cys Ser
Lys Cys Ser Pro Gly Gln His Ala Lys 50 55 60 Val Phe Cys Thr Lys
Thr Ser Asp Thr Val Cys Asp Ser Cys Glu Asp 65 70 75 80 Ser Thr Tyr
Thr Gln Leu Trp Asn Trp Val Pro Glu Cys Leu Ser Cys 85 90 95 Gly
Ser Arg Cys Ser Ser Asp Gln Val Glu Thr Gln Ala Cys Thr Arg 100 105
110 Glu Gln Asn Arg Ile Cys Thr Cys Arg Pro Gly Trp Tyr Cys Ala Leu
115 120 125 Ser Lys Gln Glu Gly Cys Arg Leu Cys Ala Pro Leu Arg Lys
Cys Arg 130 135 140 Pro Gly Phe Gly Val Ala Arg Pro Gly Thr Glu Thr
Ser Asp Val Val 145 150 155 160 Cys Lys Pro Cys Ala Pro Gly Thr Phe
Ser Asn Thr Thr Ser Ser Thr 165 170 175 Asp Ile Cys Arg Pro His Gln
Ile Cys Asn Val Val Ala Ile Pro Gly 180 185 190 Asn Ala Ser Met Asp
Ala Val Cys Thr Ser Thr Ser Pro Thr Arg Ser 195 200 205 Met Ala Pro
Gly Ala Val His Leu Pro Gln Pro Val Ser Thr Arg Ser 210 215 220 Gln
His Thr Gln Pro Thr Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser 225 230
235 240 Phe Leu Leu Pro Met Gly Pro Ser Pro Pro Ala Glu Gly Ser Thr
Gly 245 250 255 Asp Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro
Pro Cys Pro 260 265 270 Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro 275 280 285 Lys Asp Thr Leu Met Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val 290 295 300 Val Asp Val Ser His Glu Asp
Pro Glu Val Gln Phe Asn Trp Tyr Val 305 310 315 320 Asp Gly Val Glu
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 325 330 335 Phe Asn
Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln 340 345 350
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 355
360 365 Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln
Pro 370 375 380 Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu
Glu Met Thr 385 390 395 400 Lys Asn Gln Val Ser Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser 405 410 415 Asp Ile Ala Val Glu Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr 420 425 430 Lys Thr Thr Pro Pro Met
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 435 440 445 Ser Lys Leu Thr
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 450 455 460 Ser Cys
Ser Val Leu His Glu Ala Leu His Ser His Tyr Thr Gln Lys 465 470 475
480 Ser Leu Ser Leu Ser Pro Gly Lys 485
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