U.S. patent application number 14/497711 was filed with the patent office on 2015-04-02 for watery lotion skin care compositions and methods.
The applicant listed for this patent is ELC Management LLC. Invention is credited to Thomas MAMMONE, Daniel B. Yarosh.
Application Number | 20150093462 14/497711 |
Document ID | / |
Family ID | 52740401 |
Filed Date | 2015-04-02 |
United States Patent
Application |
20150093462 |
Kind Code |
A1 |
Yarosh; Daniel B. ; et
al. |
April 2, 2015 |
Watery Lotion Skin Care Compositions And Methods
Abstract
A watery lotion composition for topical application to skin
comprising at least one extract from the fermentation of the
microorganism Bifidobacterium, at least one extract from the
fermentation of the microorganism Lactobacillus, and mineral water
having at least 250 ppm of total mineral dissolved solids and a
method for improving the efficacy of a watery lotion and/or a
second skin care product by layering the products on the skin.
Inventors: |
Yarosh; Daniel B.; (Merrick,
NY) ; MAMMONE; Thomas; (Farmingdale, NY) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
ELC Management LLC |
Melville |
NY |
US |
|
|
Family ID: |
52740401 |
Appl. No.: |
14/497711 |
Filed: |
September 26, 2014 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
61884599 |
Sep 30, 2013 |
|
|
|
Current U.S.
Class: |
424/780 |
Current CPC
Class: |
A61K 2800/884 20130101;
A61K 8/99 20130101; A61K 2800/85 20130101; A61K 2800/5922 20130101;
A61K 8/965 20130101; A61Q 19/00 20130101; A61Q 19/08 20130101 |
Class at
Publication: |
424/780 |
International
Class: |
A61K 8/99 20060101
A61K008/99; A61Q 19/00 20060101 A61Q019/00 |
Claims
1. A watery lotion composition for topical application to skin
comprising at least one extract from the fermentation of the
microorganism Bifidobacterium, at least one extract from the
fermentation of the microorganism Lactobacillus, and mineral water
having at least 250 ppm of total mineral dissolved solids.
2. The composition of claim 1 wherein the extract from the
fermentation of the microorganism Bifidobacterium is Bifida ferment
extract, Bifida ferment lysate, Bifida ferment filtrate, or
mixtures thereof.
3. The composition of claim 1 wherein the extract from the
fermentation of Bifidobacterium is present at 0.01-10% of the
composition.
4. The composition of claim 1 wherein the extract from the
fermentation of Lactobacillus is present at 0.001-10% of the
composition.
5. The composition of claim 1 wherein the extract from the
fermentation of Lactobacillus is a ferment extract, ferment lysate,
or ferment filtrate.
6. The composition of claim 1 wherein the extract from the
fermentation of Lactobacillus is from one or more of the species
plantarum, casei, or crispatus.
7. The composition of claim 1 wherein the mineral water has a pH
ranging from 2.5 to 4.3, and a specific gravity of 1.0 to 1.03.
8. The composition of claim 1 wherein the watery lotion comprises
from 80-99% water.
9. A method for improving the efficacy of a watery lotion and/or
second skin care product by layering the watery lotion and second
skin care product wherein the watery lotion comprises at least one
extract from the fermentation of a microorganism from the
Lactobacillus or Bifidobacterium genus and the second skin care
product comprises at least one extract from the fermentation of
Lactobacillus or Bifidobacterium genus.
10. The method of claim 9 wherein the efficacy that is improved is
water retention in skin.
11. The method of claim 9 wherein the efficacy that is improved is
balanced skin renewal.
12. The method of claim 11 wherein the balanced skin renewal is
shown by skin having basal and epidermal layers showing a ratio of
30-70% basal cell layer and 30-70% epidermal layer.
13. The method of claim 11 wherein the balanced skin renewal is
show by skin having basal and epidermal layers showing a ratio of
40-60% basal layer and 60-40% of differentiated layer.
14. The method of claim 11 wherein the balanced skin renewal is
shown by skin having about 50% basal layer and about 50% epidermal
layer.
15. The method of claim 9 where the watery lotion comprises: 20-99%
water, 0.1-5% one or more surfactants selected from alkylene
glycols such as ethylene or propylene glycols; alkoxylated glucose,
alkoxylated alcohols, glyceryl esters; glyceryl ethers, or mixtures
thereof, 0.1-3% extract from probiotic microorganisms selected from
Lactobacillus genus, Bifidobacterium genus, or mixtures thereof,
0.1-10% of humectants selected from polyhydric alcohols, alkylene
glycols or glycerin, butylene glycol, hexylene glycol, or mixtures
thereof.
16. The method of claim 15 wherein the skin care product comprises:
20-99% water, 0.1-5% one or more surfactants selected from PEG-60,
PEG-75, PPG-5-Ceteth-20, Methyl gluceth-20, Bis-PEG-18 methyl ether
dimethyl silane, Glycereth-26, Oleth-3 phosphate, Oleth-3, Oleth-5,
Choleth-24, Ceteth-24, PEG-20 methyl glucosesesquiisostearate, and
mixtures thereof; 0.1-3% extract from probiotic microorganisms
selected from Lactobacillus genus, Bifidobacterium genus in the
form of a ferment or ferment lysate, or mixtures thereof, 0.1-10%
of humectants selected from polyhydric alcohols, alkylene glycols
or glycerin, butylene glycol, hexylene glycol, propanediol, or
mixtures thereof.
17. The method of claim 15 wherein the skin care product comprises:
20-99% water, 0.1-5% one or more surfactants selected from PEG-60,
PEG-75, PPG-5-Ceteth-20, Methyl gluceth-20, Bis-PEG-18 methyl ether
dimethyl silane, Glycereth-26, Oleth-3 phosphate, Oleth-3, Oleth-5,
Choleth-24, Ceteth-24, PEG-20 methyl glucosesesquiisostearate, and
mixtures thereof; 0.1-3% extract from probiotic microorganisms
selected from Lactobacillus genus, Bifidobacterium genus in the
form of a ferment or ferment lysate, or mixtures thereof, 0.1-10%
of humectants selected from polyhydric alcohols, alkylene glycols
or glycerin, butylene glycol, hexylene glycol, propanediol, or
mixtures thereof.
18. The method of claim 9 wherein the watery lotion comprises: 80
to 99.9% by weight of water; and at least one extract from the
fermentation of the microorganism Bifidobacterium, at least one
extract from the fermentation of the microorganism Lactobacillus,
and mineral water having at least 250 ppm of total mineral
dissolved solids.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application claims priority from U.S.
Provisional Application No. 61/884,599, filed Sep. 30, 2013.
TECHNICAL FIELD
[0002] The invention is in the field of skin care compositions and
regimens for treating skin, and more particularly, skin care
compositions in the serum or watery lotion category and regimens
for improving efficacy of individual products when they are layered
onto the skin.
BACKGROUND OF THE INVENTION
[0003] Skin care products referred to as watery lotions are very
popular, particularly with Asian skin care consumers. Watery
lotions are generally considered to be lotions or serums with no,
or a very insignificant oily phase. Most watery lotions contain
water at greater than about 85%, or even greater than 90% by weight
of the total composition. While the benefits of watery lotion
include the light fresh feel of applying mostly water to skin, the
disadvantage is that the high water content prevents inclusion of
many skin benefit active ingredients that are compatible with, and
can only be incorporated into compositions with a significant oil
phase. Thus, the need gap is to formulate watery lotions having
maximum effectiveness in treating skin while at the same time
maintaining the high water content.
[0004] Also of interest is enhancing the effectiveness of watery
lotions, not only through formulation, but using regimens where the
watery lotion and one or more additional products are layered onto
the skin to optimize efficacy without causing inhibitory activity.
More specifically, the efficacy of watery lotions can be maximized
by combining with skin care serums, especially those containing
extracts from fermentation products of microorganisms such as
Lactobacillus or Bifidobacterium.
[0005] It has been discovered that formulating watery lotions with
a certain combination of ferments and mineral containing water with
mineral concentrations greater than what is found in de-ionized
water and, in some cases, similar to what is found in blood,
provides compositions with maximum effectiveness.
[0006] In addition, it has been discovered that the efficacy of
watery lotions in providing skin benefits can be improved by
layering of two or more products onto the skin, one of which is the
watery lotion of the invention and the other being a skin care
serum, cream, or lotion, preferably containing at least one extract
from the fermentation of a microorganism from Lactobacillus or
Bifidobacterium genus.
SUMMARY OF THE INVENTION
[0007] The invention is directed to a watery lotion composition for
topical application to skin comprising an extract from the
fermentation of a probiotic microorganism from Bifidobacterium or
Lactobacillus genus and mineral water having at least 250 ppm of
total mineral dissolved solids.
[0008] The invention is directed to a regimen for improving the
efficacy of watery lotion and/or a second skin care product by
layering onto the skin a watery lotion that contains an extract
from the fermentation of a microorganism from the Bifidobacterium
or Lactobacillus genus; and a second skin care product preferably
also containing at least one extract from the fermentation of a
probiotic microorganism from the Bifidobacterium or Lactobacillus
genus. The efficacies that may be improved include, but are not
limited to, balanced cellular renewal, skin where the basal
proliferative and epidermal differentiating layers are balanced to
provide healthy, youthful skin, improved water retention, and as
further set forth herein.
[0009] The invention is also directed to a skin surface comprised
of a basal layer, an epidermal layer, a layer formed from
application of a watery lotion composition containing at least one
extract from the fermentation of a probiotic microorganisms from
the Bifidobacterium or Lactobacillus genus; and a layer formed from
application of a skin care product containing at least one extract
from the fermentation of a probiotic microorganisms from the
Bifidobacterium or Lactobacillus genus; wherein the compositions
layered onto the skin cause the basal cells in the basal layer of
the skin and the epidermal cells in the epidermal layer of the skin
to exhibit balanced proliferative and differentiating activity
respectively.
DESCRIPTION OF THE DRAWINGS
[0010] FIG. 1: depicts histological sections of skin stained with
hematoxylin & eosin (H&E), Ki-67 protein and Caspase 3
using two separate antibodies. Ki-67 indicates proliferation and
Caspase 3 indicates apoptosis. The amount of basal cell
proliferation and epidermal differentiation was shown in skin
sections that are untreated, treated with a watery lotion, treated
with a second skin care product alone, and treated with both
products. The results show that skin sections treated with watery
lotion and the second skin care product show balanced skin renewal
where the new cell generation (or proliferation) in the basal layer
and the cellular differentiation in the epidermal layer are
balanced. Where cellular proliferation and differentiation in basal
and epidermal layers is balanced, skin is most healthy, normalized
and exhibits excellent integrity.
[0011] FIG. 2: depicts histological sections of untreated skin and
skin treated with watery lotion alone, a skin care product ("Test
Formula" or "TF"), and the combination of the watery lotion and the
skin care product, stained to show cellular differentiation in the
epidermal skin layer. Similar to the results in FIG. 1, the
sections treated with watery lotion and the second skin care
product show balanced skin renewal.
[0012] FIG. 3: depicts the increase in water content of skin
treated with the combination of a watery lotion and a second skin
care product when compared to skin treated with each product alone
and untreated skin.
DETAILED DESCRIPTION
[0013] All percentages mentioned herein are percentages by weight
unless otherwise indicated.
[0014] The term "watery lotion" means an aqueous based composition
having from about 75-99% by weight of the total composition of
water.
[0015] The term "mineral water" means water having at least 250 ppm
of total mineral dissolved solids.
I. The Watery Lotion Composition
[0016] A. Mineral Water
[0017] The watery lotion composition of the invention comprises at
least about 80%, more preferably from about 80 to 99.9% by weight
of water. The water portion may comprise from 0.01 to 100%,
preferably from 0.05 to 25%, more preferably from about 0.1 to 20%
by weight of the total water portion, of mineral water having at
least 250 ppm of total dissolved mineral solids. In one preferred
embodiment the mineral water comprises concentrations of dissolved
mineral solids including but not limited to aluminum, antimony,
barium, beryllium, bismuth, boron, bromine, cadmium, calcium,
carbon, cerium, cesium, chloride, chromium, cobalt, copper,
dysprosium, erbium, europium, fluoride, gadolinium, gallium,
germanium, gold, hafnium, homium, indium, iodine, iridium, iron,
lanthanum, lithium, lutetium, magnesium manganese, molybdenum,
neodymium, niobium, osmium, palladium, phosphorus, platinum,
potassium, praseodymium, rhenium, rhodium, rubidium, ruthenium,
samarium, scandium, selenium, silicon, silver, sodium, strontium,
sulfur, tantalum, tellurium, terbium, thallium thordium, thulium,
tin, titanium, tungsten, vanadium, ytterbium, yttrium, zinc,
zirconium. In another preferred embodiment, the mineral water has a
pH ranging from 2.5 to 4.3, preferably from 2.6 to 3.9 and a
specific gravity of 1.0 to 1.03. A suitable mineral water may be
purchased from TRC Nutritional Laboratories under the trade name
77LPPM TRC Minerals.
[0018] B. Extract from Probiotic Microorganism
[0019] 1. Extract from Lactobacillus Genus
[0020] The watery lotion composition may comprise at least one
extract, preferably in the form of a ferment or ferment lysate
obtained by fermenting a microorganism from Lactobacillus. Examples
of the Lactobacillus genus include, but are not limited to,
plantarum, casei, crispatus, etc. The ferment may be in the form of
a lysate, filtrate, or both. In the case of a lysate, the
fermentation product is lysed. In the case of a filtrate, the
fermentation product is filtered. The ingredients may be purchased
from Active Concepts under the tradename AC Probiotic 1; Natural
F&P Co. Ltd under the tradename Lactobacillus crispatus KLB46;
RNA Co. under the trade name K-LAC. The ingredient may also be
purchased in the form of mixtures with other ingredients or
probiotic organisms.
[0021] The ferment may be present in amounts ranging from about
0.001 to 10%, preferably from about 0.1 to 5%, more preferably from
about 0.1 to 3%.
[0022] 2. Extract from Bifidobacterium Genus
[0023] The composition also comprises at least one extract,
preferably in the form of a ferment or ferment lysate from the
Bifidobacterium genus. Examples include Bifida ferment extract,
Bifida ferment lysate, or Bifida ferment filtrate. The fermentation
extract of Bifida may also be in the form of mixtures with other
ingredients or probiotic microorganisms. The Bifidobacterium
fermentation product may be present in the composition in amounts
ranging from about 0.01 to 10%, preferably from about 0.05 to 5%,
more preferably from about 0.1 to 2%.
[0024] C. Other Ingredients
[0025] The watery lotion may also contain other ingredients
including botanically derived actives, peptides, humectants,
surfactants, and preservatives.
[0026] 1. Peptides
[0027] Examples of suitable peptides including those having
beneficial effects on skin are acetylated peptides, such as Acetyl
hexapeptides followed by numbers ranging from 1 to 49 including all
whole integers in between. Preferred is Acetyl hexapeptide-8 (sold
under the tradename Argireline.RTM.). Also suitable are
palmitoylated peptides such as Palmitoyl oligopeptides, Palmitoyl
pentapeptides, Palmitoyl hexapeptides, Palmitoyl tetrapeptides,
Palmitoyl tripeptides. Other suitable peptides are described in the
C.T.F.A. International Cosmetic Ingredient Dictionary and Handbook,
11.sup.th Edition, 2006. If present, the peptides may range from
about 0.0001 to 3%, preferably from about 0.0005 to 2.5%, more
preferably from about 0.001 to 1%.
[0028] 2. Humectants
[0029] The composition may also comprise one or more humectants. If
present, they may range from 0.01 to 10%, preferably from 0.05 to
8%, more preferably from about 0.1 to 7%. Examples of suitable
humectants include glycols, sugars, and the like. Suitable glycols
are in monomeric or polymeric form and include polyethylene and
polypropylene glycols such as PEG 4-200, which are polyethylene
glycols having from 4 to 200 repeating ethylene oxide units; as
well as C.sub.1-6 alkylene glycols such as propylene glycol,
butylene glycol, pentylene glycol, and the like. Suitable sugars,
some of which are also polyhydric alcohols, are also suitable
humectants. Examples of such sugars include glucose, fructose,
honey, hydrogenated honey, inositol, maltose, mannitol, maltitol,
sorbitol, sucrose, xylitol, xylose, polysachharides, and so on.
Also suitable are urea and/or hyaluronic acid. Preferably, the
humectants used in the composition of the invention are C.sub.1-6,
preferably C.sub.2-4 alkylene glycols, most particularly butylene
glycol.
[0030] 3. Surfactants
[0031] The composition may contain one or more surfactants.
Preferred are nonionic organic surfactants such as alkylene
glycols, alkoxylated alcohols, or ethers, formed by the reaction of
an alcohol with an alkylene oxide, usually ethylene or propylene
oxide. Preferably the alcohol is either a fatty alcohol having 6 to
30 carbon atoms.
[0032] Examples of alkylene glycols, specifically ethylene or
propylene glycols having from about 1 to 200 repeating EO or PO
units, including all whole integers from 1 to 200. More preferred
are polyethylene glycols (PEG) having from 2 to 100 repeating
units, for example PEG 1-200 including all whole integers in
between. More specifically are PEG where the number of repeating
ethylene oxide units ranges from 40-75. Specific examples include
PEG-60, PEG-75 is preferred.
[0033] Examples of such ingredients include Steareth 2-100, which
is formed by the reaction of stearyl alcohol and ethylene oxide and
the number of ethylene oxide units ranges from 2 to 100 including
all whole integers in between; Oleth 2-100, which is formed by the
reaction of oleyl alcohol and ethylene oxide; Beheneth 5-30 which
is formed by the reaction of behenyl alcohol and ethylene oxide
where the number of repeating ethylene oxide units is 5 to 30;
Ceteareth 2-100, formed by the reaction of a mixture of cetyl and
stearyl alcohol with ethylene oxide, where the number of repeating
ethylene oxide units in the molecule is 2 to 100; Ceteth 1-45 which
is formed by the reaction of cetyl alcohol and ethylene oxide, and
the number of repeating ethylene oxide units is 1 to 45, and so
on.
[0034] Also suitable are polyethylene glycols of glycerin or
glucose such as Glycereth 2-100, gluceth 2-100 including all whole
integers in between.
[0035] Other suitable nonionic surfactants include alkoxylated
sorbitan and alkoxylated sorbitan derivatives. For example,
alkoxylation, in particular ethoxylation of sorbitan provides
polyalkoxylated sorbitan derivatives. Esterification of
polyalkoxylated sorbitan provides sorbitan esters such as the
polysorbates. For example, the polyalkyoxylated sorbitan can be
esterified with C6-30, preferably C12-22 fatty acids. Examples of
such ingredients include Polysorbates 20-85, sorbitan oleate,
sorbitan sesquioleate, sorbitan palmitate, sorbitan
sesquiisostearate, sorbitan stearate, and so on.
[0036] Also suitable are various types of betaine surfactants. Such
surfactants may be present in amounts ranging from about 0.01 to
25%, preferably from about 0.05 to 20%, more preferably from about
0.1 to 15% by weight of the total composition.
[0037] 4. Viscosity Modifiers
[0038] The composition may also contain one or more ingredients
that increase the viscosity of the composition. If present, ranges
are from about 0.01 to 10%, preferably from about 0.05 to 8%, more
preferably from about 0.1 to 5% by weight of the total composition.
Examples include synthetic polymeric thickening agents such as
carbopol, C10-30 alkyl acrylates crosspolymer, acrylates copolymer,
and ammonium acryloyldimethyltaurate/beheneth-25 copolymer, sodium
acryloyldimethyl taurate/VP copolymer, ammonium
acryloyldimethyltaurate copolymer and those sold by Clariant under
the Aristoflex.RTM. trademark.
[0039] 5. Botanical Extracts
[0040] It may be desirable to include one or more botanical
extracts in the compositions. If so, suggested ranges are from
about 0.0001 to 10%, preferably about 0.0005 to 8%, more preferably
about 0.001 to 5% by weight of the total composition. Suitable
botanical extracts include extracts from plants (herbs, roots,
flowers, fruits, seeds) such as flowers, fruits, vegetables, and so
on, including yeast ferment extract, Padina Pavonica extract,
thermus thermophilis ferment extract, camelina sativa seed oil,
Boswellia serrata extract, olive extract, Arabidopsis thaliana
extract, Acacia dealbata extract, Acer saccharinum (sugar maple),
acidopholus, acorns, aesculus, agaricus, agave, agrimonia, algae,
aloe, citrus, brassica, cinnamon, orange, apple, blueberry,
cranberry, peach, pear, lemon, lime, pea, seaweed, caffeine, green
tea, chamomile, willowbark, mulberry, poppy, and those set forth on
pages 1646 through 1660 of the CTFA Cosmetic Ingredient Handbook,
Eighth Edition, Volume 2. Further specific examples include, but
are not limited to, Glycyrrhiza glabra, Salix nigra, Macrocycstis
pyrifera, Pyrus malus, Saxifraga sarmentosa, Vitis vinifera, Morus
nigra, Scutellaria baicalensis, Anthemis nobilis, Salvia sclarea,
Rosmarinus officianalis, Citrus medica limonum, Panax ginseng,
Siegesbeckia orientalis, Fructus mume, Ascophyllum nodosum, Bifida
Ferment lysate, Glycine soja extract, Beta vulgaris, Haberlea
rhodopensis, Polygonum cuspidatum, Citrus aurantium dulcis, Vitis
vinifera, Selaginella tamariscina, Humulus lupulus, Citrus
reticulata peel, Punica granatum, Asparagopsis armata, Curcuma
longa, Menyanthes trifoliata, Helianthus annuus, Hordeum vulgare,
Cucumis sativus, Evernia prunastri, Evernia furfuracea, and
mixtures thereof.
II. The Method
[0041] The invention is directed to a regimen for improving the
efficacy of watery lotion and/or a second skin care product also
applied to skin. More specifically, the watery lotion contains an
extract from the fermentation of a probiotic microorganism from the
Bifidobacterium or Lactobacillus genus; and a second skin care
product may also containing at least one extract from the
fermentation of a probiotic microorganism from the Bifidobacterium
or Lactobacillus genus.
[0042] The efficacy that is improved includes balanced skin renewal
between basal and epidermal skin layers (where cellular
proliferation in the basal layer and cellular differentiation in
the epidermal layer are balanced), improved skin integrity,
improved skin barrier properties, improving overall skin health,
normalizing skin, and improving moisturization by causing increased
water retention in skin. Preferably, the balanced skin renewal
causes a proliferation to differentiation ratio in the basal to
epidermal layers of skin that ranges from about 30-70%
proliferation and 30-70% differentiation. More preferred is where
the proliferation to differentiation ratio in the basal to
epidermal layers ranges from 40-60% proliferation to 60-40%
differentiation. Most preferred is where the proliferation to
differentiation ratio in the basal to epidermal layers of skin is
about 50% proliferation and about 50% differentiation. This
provides optimal skin integrity, healthy and viability. In
addition, since the proliferative activity of cells in the basal
layer decreases with age, treatment according to the regiment of
the invention will cause improved proliferative activity and ensure
that the proliferative activity is balanced with cellular
differentiation in the epidermal layer to most closely approximate
the health and viability of youthful skin.
[0043] The second skin care product may be in the form of a cream,
lotion, or serum. In one preferred embodiment the second skin care
product is in the form of a serum comprising from about 20-99%
water, and from about 0.1-20% one or more surfactants, and
optionally, about 0.1-20% of one or more oils, and optionally
0.1-10% of one or more peptides, botanical extracts, viscosity
modifiers, humectants, etc. as set forth above with respect to the
watery lotion. Preferred is where the serum comprises, from about
20-99% water, 0.1-10% surfactants, 0.1-5% oil, 0.1-3% extract from
probiotic microorganism, preferably Bifidobacterium; 0.1-10% of one
or more humectants. More preferred is where the skin care product
is in the form of a serum comprising:
[0044] 20-99% water,
[0045] 0.1-5% one or more surfactants selected from alkylene
glycols such as ethylene or propylene glycols; alkoxylated glucose,
alkoxylated alcohols, glyceryl esters; glyceryl ethers, or mixtures
thereof,
[0046] 0.1-3% extract from probiotic microorganisms selected from
Lactobacillus genus, Bifidobacterium genus, or mixtures
thereof,
[0047] 0.1-10% of humectants selected from polyhydric alcohols,
alkylene glycols or glycerin, butylene glycol, hexylene glycol, or
mixtures thereof,
[0048] More preferred is where the skin care product comprises:
[0049] 20-99% water,
[0050] 0.1-5% one or more surfactants selected from polyethylene
glycols having from 40-100 repeat ethylene oxide units;
polypropylene glycols having from 3 to 100 repeat propylene glycol
units; polyethylene/polypropylene glycols having from about 40-100
repeat ethylene glycol units and 3-100 repeat propylene glycol
units; C.sub.12-40 straight or branched chain, saturated or
unsaturated alkoxylated alcohols; polyethylene glycols of glycerin;
polyethylene glycols of glucose or methyl glucose; polyethylene
glycols of cholesterol; glyceryl esters; glyceryl ethers; or
mixtures thereof.
[0051] 0.1-3% extract from probiotic microorganisms selected from
Lactobacillus genus, Bifidobacterium genus, or mixtures
thereof,
[0052] 0.1-10% of humectants selected from polyhydric alcohols,
alkylene glycols or glycerin, butylene glycol, hexylene glycol,
propanediol, or mixtures thereof.
[0053] Even more preferred is where the skin care product
comprises:
[0054] 20-99% water,
[0055] 0.1-5% one or more surfactants selected from PEG-60, PEG-75,
PPG-5-Ceteth-20, Methyl gluceth-20, Bis-PEG-18 methyl ether
dimethyl silane, Glycereth-26, Oleth-3 phosphate, Oleth-3, Oleth-5,
Choleth-24, Ceteth-24, PEG-20 methyl glucosesesquiisostearate, and
mixtures thereof;
[0056] 0.1-3% extract from probiotic microorganisms selected from
Lactobacillus genus, Bifidobacterium genus in the form of a ferment
or ferment lysate, or mixtures thereof,
[0057] 0.1-10% of humectants selected from polyhydric alcohols,
alkylene glycols or glycerin, butylene glycol, hexylene glycol,
propanediol, or mixtures thereof.
[0058] In the method of the invention, the products may be applied
in either order. Specifically, the watery lotion may be applied
first, followed by application of the second skin care product or
vice versa. The products both improve the efficacy of each other,
such that the efficacy of the watery lotion is improved when
combined with the other skin care product, and the other skin care
product efficacy is improved when combined with the watery lotion.
The efficacies that may be improved include increased water
retention in skin.
[0059] The invention will be further described in connection with
the following examples which are set forth for the purposes of
illustration only.
Example 1
[0060] A watery lotion formula was prepared as follows:
TABLE-US-00001 % by Ingredient weight Water QS100 Caffeine 0.05
Betaine (surfactant) 1.00 Trehalose (viscosity modifier) 0.50
Carbopol (viscosity modifier) 7.00 Tromethane 0.13 PEG-75
(surfactant) 3.00 Bifida ferment lysate 5.00 Acetyl glucosamine
0.50 Lactobacillus ferment 1.00 Spring (mineral) water (TRC
Nutritional Laboratories- 0.10 77LPPM TRC Minerals) Acetyl
hexpeptide-8 (peptide) 0.10 Butylene glycol (humectants) 0.50
Hyaluronic acid (humectant) 0.05 Pentylene glycol (humectant) 1.00
Propanediol (humectant) 2.00 Anthemis nobilis (Chamomile) extract
(botanical extract) 0.002 PPG-5-Ceteth-20 (non-ionic surfactant)
0.12 Butylene glycol (humectants) 1.00
[0061] The composition was prepared by combining the ingredients
and mixing well to emulsify.
Example 2
[0062] The watery lotion of Example 1 was tested along with a
commercially available skin serum formula ("Test Formula" or "TF")
with the ingredient labeling on the product as set forth below:
WATER\AQUA\EAU; BIFIDA FERMENT LYSATE; METHYL GLUCETH-20; PEG-75;
BIS-PEG-18 METHYL ETHER DIMETHYL SILANE; BUTYLENE GLYCOL;
PROPANEDIOL; COLA ACUMINATA (KOLA) SEED EXTRACT; ECHINACEA PURPUREA
(CONEFLOWER) EXTRACT; ANTHEMIS NOBILIS (CHAMOMILE); HYDROLYZED
ALGIN; PANTETHINE; CAFFEINE; LECITHIN; ETHYLHEXYLGLYCERIN; SODIUM
RNA; BISABOLOL; GLYCERETH-26; SQUALANE; SODIUM HYALURONATE; OLETH-3
PHOSPHATE; CAPRYLYL GLYCOL; LACTOBACILLUS FERMENT; OLETH-3;
OLETH-5; YEAST EXTRACT\FAEX\EXTRAIT DE LEVURE; CHOLETH-24;
HYDROGENATED LECITHIN; CETETH-24; TOCOPHERYL ACETATE; ETHYLHEXYL
METHOXYCINNAMATE; HEXYLENE GLYCOL; CARBOMER.
[0063] EFT (Epidermal Full Thickness)-300 skin models were
dispensed into 6 well plates and maintained in incubator at
37.degree. C. Daily for 48 hours wells were treated with 5 ml
EFT-300 Media (Mattek) and (1) 20 uL of Test Formula ("TF"); or (2)
20 uL of watery lotion of Example 1; or (3) 20 uL of watery lotion
applied for 1 hour followed by application of 20 uL of Test
Formula; or (4) Untreated control.
[0064] Skins were fixed in 5% formalin for 24 hours at 4.degree.
C., embedded in paraffin and sectioned at 5 uM. Sections were
stained with hematoxylin & eosin (H&E) stain to highlight
overall morphology. In addition, other sections were treated with
green (anti-Ki67) stain and red (anti-caspase 3) stain from Biocare
Medical to identify proliferating or apoptotic cells respectively.
Antibodies for filaggrin, involucrin, and transglutaminase were
purchased from Abcam.RTM. sections were exposed to antibodies to
quantify the degree of cellular differentiation in the histological
sections. Photographic images were taken at 40.times.. Scale bars
are 0.05 mm. The results are set forth in FIG. 1.
[0065] In reviewing histological sections, it is the basal layer
where cellular proliferation takes place. As these newly
proliferated cells are pushed to the epidermal layer they
differentiate. In healthy skin the basal and epidermal layers must
both show an appropriate mix of proliferation and differentiation
that should be balanced. Cellular proliferation in the basal layer
often decreases with age. As a result, cellular proliferation and
differentiation in skin tends to diminish with age. Skin "lasts
longer" and it often shows in the visual appearance of skin. In
order for skin to appear most youthful and healthy skin renewal
should be at the same or similar level that is found in young
people.
[0066] The untreated control H&E and Ki-67 Caspase stained
sections shows a basal to epidermal ratio of about 50:50. The basal
skin layer shows cellular proliferation. The upper, epidermal layer
is somewhat disrupted, but shows that cellular differentiation has
occurred.
[0067] Skin sections treated with Test Formula show an increased
basal layer that indicates that such treatment caused increased
cellular proliferation in the basal layer. The epidermal layer is
decreased in thickness compared to the untreated control, showing
that the Test Formula appears to have somewhat of an inhibitory
effect on the epidermal, differentiating, skin layer.
[0068] Skin sections treated with the watery lotion of Example 2
("WL") shows that the epidermal layer increases significantly, and
the basal layer decreases. This indicates that watery lotion
appears to promote cellular differentiation in the epidermal layer,
and has somewhat of an inhibitory effect on cellular proliferation
in the basal layer.
[0069] Skin sections treated with the combination of watery
lotion+Test Formula show an even mix of basal and epidermal skin
layers with good integrity. The sequential or layered application
of the two products promotes balanced cellular proliferation in the
basal layer and cellular differentiation in the epidermal layer.
Most unexpectedly, the inhibitory effect of the Test Formula on
cellular differentiation and the inhibitory effect of watery lotion
on cellular proliferation appears to be negated with the sequential
combination of the two products. Both the basal and epidermal
layers are compact, adherent, and histologically show a cohesive
barrier.
[0070] FIG. 2 shows skin sections marked for the presence of
filaggrin, which is another marker for cellular
differentiation.
[0071] The skin section showing untreated control demonstrates a
differentiated, but somewhat disrupted epidermal layer.
[0072] Skin section "Test Formula" shows that the application of
the Test Formula reduces the differentiated layer in the skin, thus
apparently having a slightly inhibitory effect on cellular
differentiation.
[0073] The skin section "WL" shows an increased epidermal layer
demonstrating that the watery lotion does increase cellular
differentiation in the basal layer.
[0074] The skin section "Test Formula+WL" shows that the sequential
or combined application of Test Formula and WL shows more than an
additive effect in increasing cellular differentiation in the
epidermal layer in spite of the fact that one of the products (Test
Formula) is shown to have an inhibitory effect on cellular
differentiation in the basal layer.
Example 3
[0075] The composition and method of the invention was further
tested to demonstrate increased water retention in skin using the
product combination of the invention.
[0076] EFT-300 skin models were treated apically with samples alone
or layered at day 0 and basal with 3 ml Media in a 6 well plate and
incubated at 37.degree. C. Samples were untreated control, Test
Formula from Example 2 and Watery lotion formula of Example 1
("WL").
[0077] After 24 hours the skins were placed in a MatTek permeation
device and treated apically with 200 .mu.l of 100 ppm fluorescein
in phosphate buffered saline. After 24 hours of fluorescein
penetration skins were washed in PBS, weighed wet, then dried for
24 hours and re-weighed. Data was analyzed using one way analysis
of variance (ANOVA) and significant means were tested using a
Dunnet's multiple comparison post test using GraphPad Instat
software. The results are set forth in FIG. 3, and show that skin
samples treated with the combination of watery lotion of Example 1
and the Test Formula of Example 2 exhibit substantially improved
water content retention in skin when compared to control and each
product used separately.
[0078] While the invention has been described in connection with
the preferred embodiment, it is not intended to limit the scope of
the invention to the particular form set forth but, on the
contrary, it is intended to cover such alternatives, modifications,
and equivalents as may be included within the spirit and scope of
the invention as defined by the appended claims.
* * * * *