U.S. patent application number 14/541397 was filed with the patent office on 2015-03-12 for tomato variety nun 09015 tof.
The applicant listed for this patent is Nunhems B.V.. Invention is credited to Coert Engels.
Application Number | 20150074839 14/541397 |
Document ID | / |
Family ID | 52626916 |
Filed Date | 2015-03-12 |
United States Patent
Application |
20150074839 |
Kind Code |
A1 |
Engels; Coert |
March 12, 2015 |
TOMATO VARIETY NUN 09015 TOF
Abstract
The invention provides a new and distinct hybrid variety of
tomato, NUN 09015 TOF.
Inventors: |
Engels; Coert; (AC Nunhem,
NL) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Nunhems B.V. |
AC Nunhem |
|
NL |
|
|
Family ID: |
52626916 |
Appl. No.: |
14/541397 |
Filed: |
November 14, 2014 |
Current U.S.
Class: |
800/260 ;
426/615; 426/635; 435/411; 47/58.1FV; 800/300; 800/301; 800/302;
800/303; 800/317.4 |
Current CPC
Class: |
A01H 5/08 20130101 |
Class at
Publication: |
800/260 ;
800/317.4; 435/411; 800/300; 800/301; 800/302; 800/303; 47/58.1FV;
426/615; 426/635 |
International
Class: |
A01H 5/08 20060101
A01H005/08; A01H 1/02 20060101 A01H001/02; A01G 1/00 20060101
A01G001/00; C12N 5/04 20060101 C12N005/04 |
Claims
1. A plant, plant part or seed of tomato variety NUN 09015 TOF,
wherein a representative sample of said seed has been deposited
under Accession Number NCIMB ______.
2. A plant or part thereof grown from the seed of claim 1.
3. The plant part of claim 2, further defined as a leaf, pollen, an
ovule, a fruit, a scion, a rootstock, cutting, flower or a part of
any of these or a cell.
4. A Solanum plant, or a part thereof which does not significantly
differ from the plant of claim 2 in any of the distinguishing
characteristics selected from the group consisting of 1) average
length of mature fruit (along stem axis); 2 average diameter of
mature fruit (at widest point); 3) average weight of mature fruit;
4) average number of locules; 5) an average number of flowers in
inflorescence; 6) average number of nodes between early
inflorescence; 7) typical fruit shape of type 7; 8) an average
length of dedicel (from joint to calyx attachment); 9) pubescence
on younger stems and 10) shape of blossom end.
5. A tissue or cell culture of regenerable cells of the plant of
claim 2.
6. The tissue or cell culture according to claim 5, comprising
cells or protoplasts from a plant part selected from the group
consisting of embryos, meristems, cotyledons, hypocotyl, pollen,
leaves, anthers, roots, root tips, pistil, petiole, flower, fruit,
seed, stem and stalks.
7. A tomato plant regenerated from the tissue or cell culture of
claim 5, wherein the plant has all of the physiological and
morphological characteristics of the plant of claim 2 as listed in
Table 1 when determined at the 5% significance level.
8. A tomato plant regenerated from the tissue or cell culture of
claim 5, wherein the plant has all of the physiological and
morphological characteristics of the plant of claim 2 when
determined at the 5% significance level
9. A method of producing of the plant of claim 2, or a part
thereof, comprising vegetative propagation of the plant of claim
2.
10. The method of claim 9, wherein said vegetative propagation
comprises regenerating a whole plant from a part of the plant of
claim 2.
11. The method of claim 9, wherein said part is a cutting, a cell
culture or a tissue culture.
12. A vegetative propagated plant of claim 2, or a part thereof,
wherein the plant has all of the physiological and morphological
characteristics of the plant of claim 2 when determined at the 5%
significance level.
13. A method of producing a tomato plant, comprising crossing the
plant of claim 2 with a second tomato plant one or more times, and
selecting progeny from said crossing and optionally allowing the
progeny to form seed.
14. A method of producing a tomato plant, comprising selfing the
plant of claim 2 one or more times, and selecting progeny from said
selfing, and optionally allowing the progeny to form seed.
15. Progeny of the plant of claim 2 obtained by further breeding
with said variety.
16. The progeny of claim 14, wherein said progeny have all the
distinguishing characteristics 1) to 5) or 1) to 10) of the tomato
plant of claim 2 when grown under the same environmental conditions
wherein the distinguishing characteristics are defined as 1)
average length of mature fruit (stem axis) of 58.9 to 65.1 mm; 2
average diameter of mature fruit (at widest point) of 44.6 to 49.4
mm; 3) average weight of mature fruit of 67.7 to 74.9 gram; 4)
average number of locules of 1.96 to 2.17; 5) an average number of
flowers in inflorescence of 7.3 to 8.1; 6) average number of nodes
between early inflorescence of 6.2 to 6.9 7) typical fruit shape of
type 7; 8) an average length of dedicel (from joint to calyx
attachment) of 14.8 to 16.4 mm; 9) pubescence on younger stems of
type 3--moderately hairy and 10) a type 3--nippled shape of blossom
end.
17. The progeny of claim 15 comprising all the morphological and
physiological characteristics of Table 1 when determined at the 5%
significance level.
18. A tomato plant having one, two or three physiological and/or
morphological characteristics which are different from those of the
plant of claim 2 and which otherwise has all the physiological and
morphological characteristics of the plant of claim 2 as listed in
Table 1, when determined at the 5% significance level.
19. A food or feed product comprising the plant part of claim 3
wherein the plant part can be identified as a part of the plant of
the invention.
20. A method of introducing a desired trait into a tomato plant
comprising: a) crossing a plant of claim 2 with a second tomato
plant that comprises a desired trait to produce F1 progeny; b)
selecting an F1 progeny that comprises a desired trait; c)
optionally selfing the F1 progeny one or more times to produce F2,
F3, or further generation selfing progeny, d) crossing the selected
F1 progeny or the selfing progeny with a plant of claim 2 to
produce backcross progeny; e) selecting backcross progeny
comprising the desired trait and which otherwise has all or
essentially all the physiological and morphological characteristic
of the plant of claim 2; and optionally f) repeating steps (d) and
(e) one or more times in succession to produce selected higher
backcross progeny that comprise the desired trait.
21. A tomato plant produced by the method of claim 19.
22. A tomato plant comprising at least a first set of the
chromosomes of the plant of claim 2.
23. The plant of claim 2 further comprising a single locus
conversion, wherein said plant has essentially all of the
morphological and physiological characteristics of the plant of
claim 2, optionally wherein the single locus conversion confers a
trait selected from the group consisting of male sterility,
herbicide tolerance, insect resistance, pest resistance, disease
resistance, environmental stress tolerance, modified carbohydrate
metabolism and modified protein metabolism.
24. A plant comprising the scion or rootstock of claim 3.
25. A method of producing a combination of parental lines of the
plant of claim 2 comprising the step of making double haploid cells
from haploid cells from the plant of claim 2 or a seed of claim
1.
26. A combination of parental lines produced by the method of claim
24.
27. The combination of parental lines of claim 25, from which a
seed or plant having the distinguishing characteristics 1)-5) or
1)-10) or wherein the plant has all the physiological and
morphological characteristics of the plant of claim 2 as listed in
Table 1; when the characteristics are determined at the 5%
significance level;and wherein the distinguishing characteristics
are defined as 1) average length of mature fruit (stem axis) of
58.9 to 65.1 mm; 2 average diameter of mature fruit (at widest
point) of 44.6 to 49.4 mm; 3) average weight of mature fruit of
67.7 to 74.9 gram; 4) average number of locules of 1.96 to 2.17; 5)
an average number of flowers in inflorescence of 7.3 to 8.1; 6)
average number of nodes between early inflorescence of 6.2 to 6.9
7) typical fruit shape of type 7; 8) an average length of dedicel
(from joint to calyx attachment) of 14.8 to 16.4 mm; 9) pubescence
on younger stems of type 3--moderately hairy and 10) a type
3--nippled shape of blossom end.
Description
FIELD OF THE INVENTION
[0001] The present invention relates to the field of plant breeding
and, more specifically, to the development of tomato variety NUN
09015 TOF (also designated as NUN 09015 or NUN 09015 TOF).
BACKGROUND OF THE INVENTION
[0002] The goal of vegetable breeding is to combine various
desirable traits in a single variety/hybrid. Such desirable traits
may include greater yield, resistance to diseases, insects or other
pests, tolerance to heat and drought, better agronomic quality,
higher nutritional value, enhanced growth rate and improved fruit
properties.
[0003] Breeding techniques take advantage of a plant's method of
pollination. There are two general methods of pollination: a plant
self-pollinates if pollen from one flower is transferred to the
same or another flower of the same genotype. A plant
cross-pollinates if pollen comes to it from a flower of a different
genotype.
[0004] Plants that have been self-pollinated and selected for a
uniform type over many generations become homozygous at almost all
gene loci and produce a uniform population of true breeding progeny
of homozygous plants. A cross between two such homozygous plants of
different varieties produces a uniform population of hybrid plants
that are heterozygous for many gene loci. The extent of
heterozygosity in the hybrid is a function of the genetic distance
between the parents. Conversely, a cross of two plants each
heterozygous at a number of loci produces a segregating population
of hybrid plants that differ genetically and are not uniform. The
resulting non-uniformity makes performance unpredictable.
[0005] The development of uniform varieties requires the
development of homozygous inbred plants, the crossing of these
inbred plants, and the evaluation of the crosses. Pedigree breeding
and recurrent selection are examples of breeding methods that have
been used to develop inbred plants from breeding populations. Those
breeding methods combine the genetic backgrounds from two or more
plants or various other broad-based sources into breeding pools
from which new lines are developed by selfing and selection of
desired phenotypes. The new lines are evaluated to determine which
of those have commercial potential. One crop species which has been
subject to such breeding programs and is of particular value is the
tomato.
[0006] Tomato (Solanum lycopersicum and closely related species) is
naturally a diploid and the basic chromosome number of the genus is
x=12, most are 2n=2x=24, including the cultivated ones. It
originated in the New World and has since become a mayor food crop.
In 2012, FAOSTAT estimated world production at over 160 million
tonnes.
[0007] Tomato cultivars may be grouped by maturity, i.e. the time
required from planting the seed to the stage where fruit harvest
can occur. Standard maturity classifications include `early`,
`midseason` or late-maturing`. Another classification for tomatoes
is the developmental timing of fruit set. `Determinant` plants grow
foliage, then transition into a reproductive phase of flower
setting, pollination and fruit development. Consequently,
determinant cultivars have a large proportion of the fruit ripen
within a short time frame. Growers that harvest only once in a
season favor determinant type cultivars. In contrast,
`indeterminate` types grow foliage, then enter a long phase where
flower and fruit development proceed along with new foliar growth.
Growers that harvest the same plants multiple times favor
indeterminate type cultivars. In response to more recent consumer
demands for dietary diversity, tomato breeders have developed a
wider range of colors. In addition to expanding the range of red
colored fruits, there are cultivars that produce fruits that are
creamy white, lime green, yellow, green, golden, orange and purple.
Additionally, there are multi-colored varieties exemplified by
mainly red fruited varieties with green shoulders, and both
striped- and variegated-colored fruit.
[0008] The fruits of tomato plants which are more suitable for
processing are generally red colored and have pink to red/crimson
fruit flesh.
SUMMARY OF THE INVENTION
[0009] The invention concerns seed of tomato variety NUN 09015 TOF,
wherein a representative sample of said seed has been deposited
under Accession Number NCIMB ______. The tomato seed of the
invention may be provided as an essentially homogeneous population
of tomato seed. Therefore, seed of the invention may be defined as
forming at least about 97% of the total seed, including at least
about 98%, 99% or more of the seed. The population of tomato seed
may be particularly defined as being essentially free from other
seed. The seed population may be separately grown to provide an
essentially homogeneous population of tomato plants according to
the invention. Also encompassed are plants grown from seeds of
tomato variety NUN 09015 TOF and plant parts thereof.
[0010] In another aspect the invention provides for a hybrid
variety of S. lycopersicum called NUN 09015 TOF. The invention also
provides for a plurality of seeds of the new variety, plants
produced from growing the seeds of the new variety NUN 09015 TOF,
and progeny of any of these. Especially, progeny retaining one or
more (or all) of the "distinguishing characteristics" or one or
more (or all) of the "essential morphological and physiological
characteristics" or essentially all physiological and morphological
characteristics of NUN 09015 TOF referred to herein, are
encompassed herein as well as methods for producing these.
[0011] In one aspect, such progeny have all the physiological and
morphological characteristics of tomato variety NUN 09015 TOF when
grown under the same environmental conditions. In another aspect
such progeny have all the physiological and morphological
characteristics as listed in Table 1 as tomato variety NUN 09015
TOF when measured under the same environmental conditions (i.e.
evaluated at significance levels of 1%, 5% or 10% significance,
which can also be expressed as a p value).
[0012] In another aspect a plant of the invention or said progeny
plants has/have 1, 2, 3, 4 or more or all of the distinguishing
characteristics: 1) average length of mature fruit (stem axis) of
58.9 to 65.1 mm; 2 average diameter of mature fruit (at widest
point) of 44.6 to 49.4 mm; 3) average weight of mature fruit of
67.7 to 74.9 gram; 4) average number of locules of 1.96 to 2.17; 5)
an average number of flowers in inflorescence of 7.3 to 8.1; 6)
average number of nodes between early inflorescence of 6.2 to 6.9
7) typical fruit shape of type 7; 8) an average length of dedicel
(from joint to calyx attachment) of 14.8 to 16.4 mm; 9) pubescence
on younger stems of type 3--moderately hairy and 10) a type
3--nippled shape of blossom end, in addition to 3, 4, 5, 6, 7, 8,
or more, or all of the other (average) characteristics as listed in
Table 1.
[0013] Further, a tomato fruit produced on a plant grown from these
seeds is provided.
[0014] In yet another embodiment of the invention, a plant having
one, two or three physiological and/or morphological
characteristics which are different from those of NUN 09015 TOF and
which otherwise has all the physiological and morphological
characteristics of NUN 09015 TOF as listed in Table 1, wherein a
representative sample of seed of variety NUN 09015 TOF has been
deposited under Accession Number NCIMB ______, is provided.
[0015] Further, a vegetatively propagated plant of variety NUN
70038 PPH, or a part thereof, is provided having all the
morphological and physiological characteristics of NUN 09015 TOF
when grown under the same environmental conditions.
[0016] Also a plant part derived from variety NUN 70038 is
provided, wherein said plant part is selected from the group
consisting of: fruit, harvested fruit, parts of fruits, leaf,
pollen, ovule, cell, part of a leaf, petioles, shoots or parts
thereof, stems or parts thereof, roots or parts thereof, cuttings,
seeds, hypocotyl, cotyledon, flowers or parts thereof, scion,
scion, stock, rootstock and flower. Fruits are particularly
important plant parts.
DEFINITIONS
[0017] "Tomato" refers herein to plants of the species Solanum
lycopersicum, or a closely related species, and fruits thereof.
Solanum lycopersicum, is also known as Lycopersicon lycopersicum
(L.) H. Karst. or Lycopersicon esculentum Mill.
[0018] "Cultivated tomato" refers to plants of Solanum
lycopersicum, or a closely related species, i.e. varieties,
breeding lines or cultivars of the species S. lycopersicum as well
as crossbreds thereof, or crossbreds with other Solanum species,
cultivated by humans and having good agronomic characteristics;
preferably such plants are not "wild plants", i.e. plants which
generally have much poorer yields and poorer agronomic
characteristics than cultivated plants and e.g. grow naturally in
wild populations. "Wild plants" include for example ecotypes, PI
(Plant Introduction) lines, landraces or wild accessions or wild
relatives of Solanum and related species.
[0019] The terms "tomato plant designated NUN 09015 TOF", "NUN
09015", "09015 TOF" or "variety designated 09015 TOF" are used
interchangeably herein and refer to a tomato plant of variety NUN
09015 TOF, representative seed of which having been deposited under
Accession Number NCIMB ______.
[0020] "Tissue culture" refers to a composition comprising isolated
cells of the same or a different type or a collection of such cells
organized into parts of a plant. Tissue culture of various tissues
of tomato and regeneration of plants therefrom is well known and
widely published (see, e.g., Bhatia et al. (2004), Plant Cell,
Tissue and Organ Culture 78: 1-21. Similarly, the skilled person is
well-aware how to prepare a "cell culture".
[0021] "UPOV descriptors" are the plant variety descriptors
described for tomato in the "Guidelines for the Conduct of Tests
for Distinctness, Uniformity and Stability, TG/44/10 (Geneva 2001),
as published by UPOV (International Union for the Protection of New
Varieties and Plants, available on the world wide web at upov.int)
and which can be downloaded from the world wide web at
upov.int/en/publications/tg-rom/tg044/tg.sub.--44.sub.--10.pdf and
is herein incorporated by reference in its entirety.
[0022] "USDA descriptors" are the plant variety descriptors for
tomato (Solanum lycopersicum or Lycopersicon esculentum Mill.) as
published by the US Department of Agriculture, Agricultural
Marketing Service, Plant Variety Protection Office, Beltsville, Md.
20705 (available on the world wide web at ams.usda.gov) and which
can be downloaded from the world wide web at
ams.usda.gov/AMSv1.0/getfile?dDocName=STELDEV3003738.
[0023] "RHS" refers to the Royal Horticultural Society of England
which publishes an official botanical color chart quantitatively
identifying colors according to a defined numbering system. The
chart may be purchased from Royal Horticulture Society Enterprise
Ltd RHS Garden; Wisley, Woking; Surrey GU236QB, UK, e.g., the RHS
colour chart: 2007 (The Royal Horticultural Society, charity No:
222879, PO Box 313 London SW1P2PE; sold by, e.g., TORSO-VERLAG,
Obere Gruben 8 .cndot. D-97877 Wertheim, Article-No.: Art62-00008
EAN-Nr.: 4250193402112).
[0024] As used herein, the term "plant" includes the whole plant or
any parts or derivatives thereof, preferably having the same
genetic makeup as the plant from which it is obtained, such as
plant organs (e.g. harvested or non-harvested fruits), plant cells,
plant protoplasts, plant cell tissue cultures or tissue cultures
from which whole plants can be regenerated, plant calli, plant cell
clumps, plant transplants, seedlings, hypocotyl, cotyledon, plant
cells that are intact in plants, plant clones or micropropagations,
or parts of plants (e.g. harvested tissues or organs), such as
plant cuttings, vegetative propagations, embryos, pollen, ovules,
fruits, flowers, leaves, seeds, clonally propagated plants, roots,
stems, root tips, grafts, scions, rootstocks, parts of any of these
and the like. Also any developmental stage is included, such as
seedlings, cuttings prior or after rooting, mature plants or
leaves.
[0025] "Harvested plant material" refers herein to plant parts
(e.g. fruits detached from the whole plant) which have been
collected for further storage and/or further use.
[0026] "Harvested seeds" refers to seeds harvested from a line or
variety, e.g. produced after self-fertilization or
cross-fertilization and collected.
[0027] "Internode" refers to a portion of a plant stem between
nodes.
[0028] "Node" refers to the place on a plant stem where a leaf is
attached.
[0029] "Rootstock" or "stock" refers to the plant selected for its
roots, in particular for the resistance of the roots to diseases or
stress (e.g. heat, cold, salinity etc). Normally the quality of the
fruit of the plant providing the rootstock is less important.
[0030] "Scion" refers to a part of the plant that is attached to
the rootstock. This plant is selected for its stems, leaves,
flowers, or fruits. The scion contains the desired genes to be
duplicated in future production by the stock/scion plant and may
produce the desired tomato fruit.
[0031] "Stock/scion" plant refers to a tomato plant comprising a
rootstock from one plant grafted to a scion from another plant.
[0032] "Grafting" refers to attaching tissue from one plant to
another plant so that the vascular tissues of the two tissues join
together. Grafting may be done using methods known in the art like:
Tongue Approach/Approach Graft, 2) Hole insertion/Terminal/Top
Insertion Graft, 3) One Cotyledon/Slant/Splice/Tube Graft and 4)
Cleft/Side Insertion Graft
[0033] A plant having "all the physiological and morphological
characteristics" of a referred-to-plant means a plant having the
physiological and morphological characteristics of the
referred-to-plant when grown under the same environmental
conditions, preferably in the same experiment; the
referred-to-plant can be a plant from which it was derived, e.g.
the progenitor plant, the parent, the recurrent parent, the plant
used for tissue- or cell culture, etc.
[0034] A plant having "essentially all the physiological and
morphological characteristics" of a referred-to-plant means a plant
having all the (e.g. 1-10) distinguishing physiological and
morphological characteristics (distinguishing characteristics as
herein defined) when grown under the same environmental conditions
of the referred-to-plant (e.g. a plant from which it was derived
such as the progenitor plant, the parent, the recurrent parent, the
plant used for tissue- or cell culture, etc.) Alternatively, a
plant having "essentially all the physiological and morphological
characteristics" of a referred-to-plant means a plant having all
the characteristics as listed in Table 1 when grown under the same
environmental conditions as a referred-to-plant (e.g. a plant from
which it was derived such as the progenitor plant, the parent, the
recurrent parent, the plant used for tissue- or cell culture,
etc.). In another embodiment, a plant having "essentially all the
physiological and morphological characteristics" of a
referred-to-plant means a plant having all but 1, 2, 3, 4 or 5 of
the characteristics as listed in Table 1 when grown under the same
environmental conditions as a referred-to-plant (e.g. a plant from
which it was derived such as the progenitor plant, the parent, the
recurrent parent, the plant used for tissue- or cell culture,
etc.).
[0035] For NUN 09015 TOF the distinguishing characteristics are 1)
average length of mature fruit (along stem axis); 2 average
diameter of mature fruit (at widest point); 3) average weight of
mature fruit; 4) average number of locules; 5) an average number of
flowers in inflorescence; 6) average number of nodes between early
inflorescence; 7) typical fruit shape of type 7; 8) an average
length of dedicel (from joint to calyx attachment); 9) pubescence
on younger stems and 10) shape of blossom end.
[0036] In certain embodiments the plant of the invention has all
the physiological and morphological characteristics, except for
certain characteristics mentioned, e.g. the characteristic(s)
derived from a converted or introduced gene or trait and/or except
for the characteristics which differ.
[0037] Similarity between different plants is defined as the number
of distinguishing characteristics (or the characteristics as listed
in Table 1) that are the same between the two plants that are
compared when grown under the same environmental conditions.
Characteristics are considered "the same" when the value for a
numeric characteristic is evaluated at significance levels of 1%,
5% or 10% significance level, or when a non-numeric characteristic
is identical, if the plants are grown under the same
conditions.
[0038] A plant having one or more "essential physiological and/or
morphological characteristics" or one or more "distinguishing
characteristics" refers to a plant having (or retaining) one or
more of the characteristics mentioned in Table 1 when grown under
the same environmental conditions that distinguish NUN 09015 TOF
from the most similar varieties (such as variety Daniela), such as
but not limited to average number of fruits per plant, heat of
(dried) fruit, maturity, average fruit diameter at mid-point or
average fruit length.
[0039] "Distinguishing characteristics" or "distinguishing
morphological and/or physiological characteristics" refers herein
the characteristics which are distinguishing between NUN 09015 TOF
and other tomato varieties, such as Daniela, when grown under the
same environmental conditions, especially the following
characteristics: 1) an average length of mature fruit (stem axis)
of 58.9 to 65.1 mm; 2 average diameter of mature fruit (at widest
point) of 44.6 to 49.4 mm; 3) average weight of mature fruit of
67.7 to 74.9 gram; 4) average number of locules of 1.96 to 2.17; 5)
an average number of flowers in inflorescence of 7.3 to 8.1; 6)
average number of nodes between early inflorescence of 6.2 to 6.9
7) typical fruit shape of type 7; 8) an average length of dedicel
(from joint to calyx attachment) of 14.8 to 16.4 mm; 9) pubescence
on younger stems of type 3--moderately hairy and 10) a type
3--nippled shape of blossom end. In one aspect, the distinguishing
characteristics further include at least one, two, three or more
(or all) of the characteristics listed in Table 1. All numerical
distinguishing characteristics are statistically significantly
different at p<0.05.
[0040] Thus, a tomato plant "comprising the distinguishing
characteristics of "NUN 09015 TOF" refers herein to a tomato plant
which does not differ significantly from NUN 09015 TOF in
characteristics 1) to 5) above. In a further aspect the tomato
plant further does not differ significantly from NUN 09015 TOF in
one or more, or all characteristics 6) to 10) as mentioned above.
In yet a further aspect the tomato plant further does not differ in
at least one, two, three, four, five or six (or all)
characteristics selected from the characteristics listed in Table
1. In still another aspect the tomato plant does not differ in any
of the distinguishing characteristics 1) to 10) listed above.
[0041] The physiological and/or morphological characteristics
mentioned above are commonly evaluated at significance levels of
1%, 5% or 10%, when measured under the same environmental
conditions. For example, a progeny plant of NUN 09015 TOF may have
one or more (or all) of the essential physiological and/or
morphological characteristics of NUN 09015 TOF listed in Table 1,
as determined at the 5% significance level when grown under the
same environmental conditions.
[0042] As used herein, the term "variety", "cultivated tomato" or
"cultivar" means a plant grouping within a single botanical taxon
of the lowest known rank, which grouping, irrespective of whether
the conditions for the grant of a breeder's right are fully met,
can be defined by the expression of the characteristics resulting
from a given genotype or combination of genotypes, distinguished
from any other plant grouping by the expression of at least one of
the said characteristics and considered as a unit with regard to
its suitability for being propagated unchanged.
[0043] A variety is referred to as an "Essentially Derived Variety"
(EDV) i.e., shall be deemed to be essentially derived from another
variety, "the initial variety" when (i) it is predominantly derived
from the initial variety, or from a variety that is itself
predominantly derived from the initial variety, while retaining the
expression of the essential characteristics that result from the
genotype or combination of genotypes of the initial variety; (ii)
it is clearly distinguishable from the initial variety; and (iii)
except for the differences which result from the act of derivation,
it conforms to the initial variety in the expression of the
essential characteristics that result from the genotype or
combination of genotypes of the initial variety. Thus, an EDV may
be obtained for example by the selection of a natural or induced
mutant, or of a somaclonal variant, the selection of a variant
individual from plants of the initial variety, backcrossing, or
transformation by genetic engineering.
[0044] "Plant line" is for example a breeding line which can be
used to develop one or more varieties. Progeny obtained by selfing
a plant line has the same phenotype as its parents.
[0045] "Hybrid variety" or "F1 hybrid" refers to the seeds
harvested from crossing two inbred (nearly homozygous) parental
lines. For example, the female parent is pollinated with pollen of
the male parent to produce hybrid (F1) seeds on the female
parent.
[0046] "Regeneration" refers to the development of a plant from
cell culture or tissue culture or vegetative propagation.
[0047] "Vegetative propagation", "vegetative reproduction" or
"clonal propagation" are used interchangeably herein and mean the
method of taking part of a plant and allowing that plant part to
form at least roots where plant part is, e.g., defined as or
derived from (e.g. by cutting of) leaf, pollen, embryo, cotyledon,
hypocotyl, cells, protoplasts, meristematic cell, root, root tip,
pistil, anther, flower, shoot tip, shoot, stem, fruit, petiole,
etc. When a whole plant is regenerated by vegetative propagation,
it is also referred to as a vegetative propagation.
[0048] "Selfing" refers to self-pollination of a plant, i.e., the
transfer of pollen from the anther to the stigma of the same
plant.
[0049] "Crossing" refers to the mating of two parent plants. The
term encompasses "cross-pollination" and "selfing".
[0050] "Cross-pollination" refers to the fertilization by the union
of two gametes from different plants.
[0051] "Substantially equivalent" refers to a characteristic that,
when compared, does not show a statistically significant difference
(e.g., p=0,05) from the mean. ANOVA is a suitable method for
determining the value of p (Clewer, A. G., and D. H. Scarisbrick.
2001).
[0052] "Locus" (plural loci) refers to the specific location of a
gene or DNA sequence on a chromosome. A locus may confer a specific
trait.
[0053] "Allele" refers to one or more alternative forms of a gene
locus. All of these loci relate to one trait. Sometimes, different
alleles can result in different observable phenotypic traits, such
as different pigmentation. However, many variations at the genetic
level result in little or no observable variation. If a
multicellular organism has two sets of chromosomes, i.e. diploid,
these chromosomes are referred to as homologous chromosomes.
Diploid organisms have one copy of each gene (and therefore one
allele) on each chromosome. If both alleles are the same, they are
homozygotes. If the alleles are different, they are
heterozygotes.
[0054] "Genotype" refers to the genetic composition of a cell or
organism.
[0055] "Phenotype" refers to the detectable characteristics of a
plant, cell or organism, which characteristics are the
manifestation of gene expression.
[0056] Haploid" refers to a cell or organism having one set of the
two sets of chromosomes in a diploid.
[0057] "Diploid" refers to a cell or organism having two sets of
chromosomes.
[0058] "Polyploid" refers to a cell or organism having three or
more complete sets of chromosomes.
[0059] "Triploid" refers to a cell or organism having three sets of
chromosomes.
[0060] "Tetraploid" refers to a cell or organism having four sets
of chromosomes.
[0061] "Maturity" refers to the fruit developmental stage when the
fruit has fully developed (reached its final size), begins to ripen
and undergoes ripening, during which fruits can be divided into 1,
2, 3 or more maturity stages. Thereafter, fruits become overripe.
In particular embodiments "maturity" is defined as the mature stage
of fruit development and optimal time for harvest. In one
embodiment a "mature" tomato is defined as having reached the stage
of maturity which will insure the proper completion of the normal
ripening process. In particular embodiments, fruit should be
harvested at a maturity stage i.e. substantially near maximum
sweetness and flavor intensity.
[0062] "Harvest maturity" is referred to as the stage at which a
tomato fruit is ripe or ready for harvest or the optimal time to
harvest the fruit. In one embodiment, harvest maturity is the stage
which allows proper completion of the normal ripening.
[0063] "Flavor" (or flavour) refers to the sensory impression of a
food or other substance, especially a tomato fruit or fruit part
(fruit flesh) and is determined mainly by the chemical senses of
taste and smell. Flavor is influenced by texture properties and by
volatile and/or non-volatile chemical components (organic acids,
lipids, carbohydrates, salts etc.).
[0064] The term "traditional breeding techniques" encompasses
herein crossing, selfing, selection, double haploid production,
embryo rescue, protoplast fusion, marker assisted selection,
mutation breeding etc. as known to the breeder (i.e. methods other
than genetic modification/transformation/transgenic methods), by
which, for example, a genetically heritable trait can be
transferred from one tomato line or variety to another.
[0065] "Backcrossing" is a traditional breeding technique used to
introduce a trait into a plant line or variety. The plant
containing the trait is called the donor plant and the plant into
which the trait is transferred is called the recurrent parent. An
initial cross is made between the donor parent and the recurrent
parent to produce progeny plants. Progeny plants which have the
trait are then crossed to the recurrent parent. After several
generations of backcrossing and/or selfing the recurrent parent
comprises the trait of the donor. The plant generated in this way
may be referred to as a "single trait converted plant".
[0066] "Progeny" as used herein refers to plants derived from a
plant designated NUN 09015 TOF. Progeny may be derived by
regeneration of cell culture or tissue culture or parts of a plant
designated NUN 09015 TOF or selfing of a plant designated NUN 09015
TOF or by producing seeds of a plant designated NUN 09015 TOF. In
further embodiments, progeny may also encompass plants derived from
crossing of at least one plant designated NUN 09015 TOF with
another tomato plant of the same or another variety or (breeding)
line, or wild tomato plants, backcrossing, inserting of a locus
into a plant or mutation. A progeny is, e.g., a first generation
progeny, i.e. the progeny is directly derived from, obtained from,
obtainable from or derivable from the parent plant by, e.g.,
traditional breeding methods (selfing and/or crossing) or
regeneration. However, the term "progeny" generally encompasses
further generations such as second, third, fourth, fifth, sixth,
seventh or more generations, i.e., generations of plants which are
derived from, obtained from, obtainable from or derivable from the
former generation by, e.g., traditional breeding methods,
regeneration or genetic transformation techniques. For example, a
second generation progeny can be produced from a first generation
progeny by any of the methods mentioned above.
[0067] The terms "gene converted" or "conversion plant" in this
context refer to tomato plants which are developed by backcrossing
wherein essentially all of the desired morphological and
physiological characteristics of parent are recovered in addition
to the one or more genes transferred into the parent via the
backcrossing technique or via genetic engineering. Likewise a
"Single Locus Converted (Conversion) Plant" refers to plants which
are developed by plant breeding techniques comprising or consisting
of backcrossing, wherein essentially all of the desired
morphological and physiological characteristics of a tomato variety
are recovered in addition to the characteristics of the single
locus having been transferred into the variety via the backcrossing
technique and/or by genetic transformation.
[0068] "Transgene" or "chimeric gene" refers to a genetic locus
comprising a DNA sequence which has been introduced into the genome
of a tomato plant by transformation. A plant comprising a transgene
stably integrated into its genome is referred to as "transgenic
plant".
[0069] "Linkage" refers to a phenomenon wherein alleles on the same
chromosome tend to segregate together more often than expected by
chance if their transmission was independent.
[0070] "Marker" refers to a readily detectable phenotype,
preferably inherited in codominant fashion (both alleles at a locus
in a diploid heterozygote are readily detectable), with no
environmental variance component, i.e., a heritability of 1.
[0071] "Average" refers herein to the arithmetic mean.
[0072] The term "mean" refers to the arithmetic mean of several
measurements. The skilled person understands that the appearance of
a plant depends to some extent on the growing conditions of said
plant. Thus, the skilled person will know typical growing
conditions for tomatoes described herein. The mean, if not
indicated otherwise within this application, refers to the
arithmetic mean of measurements on at least 10 different, randomly
selected plants of a variety or line.
DETAILED DESCRIPTION
[0073] The present invention relates to a Solanum lycopersicum
variety, referred to as NUN 09015 TOF, which has a higher average
number of seeds per fruit, greater heat of (dried) fruit, earlier
maturity, lower average plant height, lower average plant width,
lower average seed cavity diameter, concentrated fruit set instead
of scattered fruit set, higher average placental length, lower
average fruit flesh thickness and higher average third internode
length than check variety Daniela. Also encompassed by the present
invention are progeny plants having all but 1, 2, or 3 of the
morphological and/physiological characteristics of NUN 09015 TOF
and methods of producing plants in accordance with the present
invention.
[0074] A tomato plant of NUN 09015 TOF differs from the most
similar comparison variety Daniela in one or more characteristics
(referred herein to as "distinguishing characteristics" or
"distinguishing morphological and/or physiological characteristics"
(or essential physiological and/or morphological characteristics)
selected from: [0075] 1) NUN 09015 TOF has an average length of
mature fruit (stem axis) that is at least 10%, or preferably 15,
20, 25, 26% or even more preferably about 27% higher than Daniela;
[0076] 2) NUN 09015 TOF has an average diameter of mature fruit (at
widest point) that is at least 10%, or preferably 15, 16, 225, 17,
18% or even more preferably about 18.7% lower than Daniela; [0077]
3) NUN 09015 TOF has an average weight of mature fruit that is at
least 10%, or preferably 15, 20, 21, 22% or even more preferably
about 22.9% lower than Daniela; [0078] 4) NUN 09015 TOF has an
average number of locules that is at least 20%, or preferably 25,
30, 35, 36% or even more preferably about 37% lower than Daniela;
[0079] 5) NUN 09015 TOF has an average number of flowers in
inflorescence that is at least 15%, or preferably 20, 25, 27.5, 30%
or even more preferably about 31% higher than Daniela; [0080] 6)
NUN 09015 TOF has an average number of nodes between early
inflorescence that is at least 10%, or preferably 12, 13, 15, 16%
or even more preferably about 16.6% higher than Daniela; [0081] 7)
NUN 09015 TOF has a typical fruit shape of type 7 instead of a type
2 which Daniela has; [0082] 8) NUN 09015 TOF has an average length
of dedicel (from joint to calyx attachment) that is at least 10%,
or preferably 12 14, 15, 16% or even more preferably about 17%
longer than Daniela; [0083] 9) NUN 09015 TOF has pubescence on
younger stems of type 3--moderately hairy instead of a type
2--sparsely hairy which Daniela has; and [0084] 10) NUN 09015 TOF
has a type 3--nippled shape of blossom end instead of a type
2--flat which Daniela has.
[0085] It is understood that "significant" differences refer to
statistically significant differences, when comparing the
characteristic between two plant lines or varieties when grown
under the same conditions. Preferably at least about 10, 15, 20 or
more plants per line or variety are grown under the same conditions
(i.e. side by side) and characteristics are measured on at least
about 10, 15, 20 or more randomly selected plant or plant parts to
obtain averages. Thus, physiological and morphological
characteristics or traits are commonly evaluated at a significance
level of 1%, 5% or 10%, when measured in plants grown under the
same environmental conditions.
[0086] Thus, in one aspect, the invention provides seeds of the
tomato variety designated NUN 09015 TOF wherein a representative
sample of seeds of said variety was deposited under the Budapest
Treaty, with Accession number NCIMB ______.
[0087] Seeds of NUN 09015 TOF are obtainable by crossing the male
parent with the female parent and harvesting the seeds produced on
the female parent. The resultant NUN 09015 TOF seeds can be grown
to produce NUN 09015 TOF plants. In one embodiment a plurality of
NUN 09015 TOF seeds are packaged into small and/or large containers
(e.g., bags, cartons, cans, etc.). The seeds may be disinfected,
primed and/or treated with various compounds, such as seed coatings
or crop protection compounds.
[0088] Also provided are plants of tomato variety NUN 09015 TOF, or
a fruit or other plant part thereof, produced from seeds, wherein a
representative sample of said seeds has been deposited under the
Budapest Treaty, with Accession Number NCIMB ______. Also included
is a cell culture or tissue culture produced from such a plant or a
plant regenerated from such a cell or tissue culture said plant
expressing all the morphological and physiological characteristics
of NUN 09015 TOF.
[0089] Plants of NUN 09015 TOF can be produced by seeding directly
in the ground (e.g., field) or by germinating the seeds in
controlled environment conditions (e.g., greenhouses) and then
transplanting the seedlings into the field. For example, the seed
can be sown into prepared seed beds where they will remain for the
entire production of the crop. Alternatively, the tomato seed may
be planted through a black plastic mulch. The dark plastic will
absorb heat from the sun, warming the soil early. It will also help
to conserve moisture during the growing season, controls weeds and
makes harvesting easier and cleaner. Tomato can also be grown
entirely in greenhouses. See for example M Domis, A P Papadopoulos
(2002) Horticultural Reviews for cultivation, harvesting, handling
and postharvest methods commonly used.
[0090] In another aspect, the invention provides for a tomato plant
of variety NUN 09015 TOF, a representative sample of seed from said
variety has been deposited under the Budapest Treaty, with
Accession number NCIMB ______.
[0091] In other aspects, the invention provides for a fruit of
tomato variety NUN 09015 TOF, or a plant part, such as pollen,
flowers, shoots or cuttings of variety NUN 09015 TOF or parts
thereof.
[0092] In one embodiment any plant of the invention comprises at
least 3, 4, 5 or more, e.g. 6, 7, 8, 9 or all of the following
morphological and/or physiological characteristics (i.e.
distinguishing characteristics (average values; measured at harvest
or market maturity, as indicated on the USDA Objective description
of variety--Tomato (unless indicated otherwise), when grown under
the same environmental conditions): [0093] 1) NUN 09015 TOF has an
average length of mature fruit (stem axis) of about 62 mm e.g.
between about 45 and about 80 or preferably between about 50 and
about 75 or between about 55 and 70 or even between about 60 and 65
seeds; 1) of 58.9 to 65.1 mm; 2 of 44.6 to 49.4 mm; 3) of 67.7 to
74.9 gram; 4) average of 1.96 to 2.17; 5) an of 7.3 to 8.1; 6) of
6.2 to 6.9 7; 8) an average of 14.8 to 16.4 mm; 9) pubescence on
younger stems of type 3--moderately hairy and 10) a type 3--nippled
shape of blossom end. [0094] 2) NUN 09015 TOF has an average
diameter of mature fruit (at widest point) of about 47 mm e.g.
between about 30 mm and about 65 mm or preferably between about 35
and about 60 mm or between about 40 and 55 mm or even between about
45 and 50 mm; [0095] 3) NUN 09015 TOF has average weight of mature
fruit of about 71.3 gram e.g. between about 65 and about 80 g or
preferably between about 68 and about 77 g or between about 70 and
74 g or even between about 71 and 72 g; [0096] 4) NUN 09015 TOF has
an average number of locules of class 1: approximately two locules;
[0097] 5) NUN 09015 TOF has an average number of flowers in
inflorescence of about 7.7 e.g. between about 6.5 and about 9.0 or
preferably between about 7.0 and about 8.5 or between about 7.5 and
8.0 or even between about 7.6 and 7.8. [0098] 6) NUN 09015 TOF has
an average number of nodes between early inflorescence of about
6.53 e.g. between about 6 and about 8 or preferably between about
6.5 and about 7.5 or between about 6.00 and 7.00 or even between
about 6.25 and 6.75; [0099] 7) NUN 09015 TOF has a typical fruit
shape of type 7; [0100] 8) NUN 09015 TOF has an average length of
dedicel (from joint to calyx attachment) of about 15.6 mm e.g.
between about 10 mm and about 20 mm or preferably between about 12
and about 18 mm or between about 14 and 17 mm or even between about
15 and 16 mm; [0101] 9) NUN 09015 TOF has pubescence on younger
stems that is type 3--moderately hairy; and [0102] 10) NUN 09015
TOF has a type 3--nippled shape of blossom end of the fruit.
[0103] In another embodiment, NUN 09015 TOF has intermediate to
high resistance against the viral diseases Tomato Mosaic Virus
strain 0,1 and 2 and the fungal diseases Verticillium dahlia Race
0, Fusarium oxysporum f. sp. Lycopersici race 0 and 1, Cladosporium
fulvum race 0 group A, B, C, D, and E, All resistances are
classified as high (9 out of 9, with 9 highest. All Rates are from
1 (most susceptible) to 9 (most resistant.
[0104] In still another aspect the invention provides a method of
producing a tomato plant, comprising crossing a plant of tomato
variety NUN 09015 TOF with a second tomato plant one or more times,
and selecting progeny from said crossing.
[0105] In yet another aspect the invention provides a method of
producing a tomato plant, comprising selfing a plant of tomato
variety NUN 09015 TOF one or more times, and selecting progeny from
said selfing.
[0106] In other aspects, the invention provides for progeny of
variety NUN 09015 TOF such as progeny obtained by further breeding
NUN 09015 TOF. Further breeding NUN 09015 TOF includes selfing NUN
09015 TOF one or more times and/or cross-pollinating NUN 09015 TOF
with another tomato plant or variety one or more times. In
particular, the invention provides for progeny that retain all the
essential morphological and physiological characteristics of NUN
09015 TOF or that retain one or more of the distinguishing
characteristics of the tomato type described further above and when
grown under the same environmental conditions. In another aspect,
the invention provides for vegetative reproductions of the variety
and plants having all but 1, 2, or 3 of the physiological and
morphological characteristics of NUN 09015 TOF (e.g. as listed in
Table 1).
[0107] The morphological and/or physiological differences between
plants according to the invention, i.e. NUN 09015 TOF or progeny
thereof, or plants having all but 1, 2, or 3 of the physiological
and morphological characteristics of NUN 09015 TOF (as listed in
Table 1); and other known varieties can easily be established by
growing NUN 09015 TOF next to the other varieties (in the same
field, under the same environmental conditions), preferably in
several locations which are suitable for said tomato cultivation,
and measuring morphological and/or physiological characteristics of
a number of plants (e.g., to calculate an average value and to
determine the variation range/uniformity within the variety). For
example, trials can be carried out in Acampo Calif., USA (N 38
degrees 07'261''/W 121 degrees 18'807'', USA, whereby, maturity,
days from seeding to harvest, plant habit, plant attitude, leaf
shape, leaf color, blistering, numbers of flowers per leaf axil,
number of calyx lobes, number of petals, fruit group, immature
fruit color, mature fruit color, pungency, flavor, fruit
glossiness, fruit size, fruit shape, average number of fruits per
plant, seed size, seed weight, anthocyanin level, disease
resistance, insect resistance, can be measured and directly
compared for species of Solanum.
[0108] The morphological and physiological characteristics (and
distinguishing characteristics) of NUN 09015 TOF, are provided in
the Examples, in Table 1. Encompassed herein are also plants
derivable from NUN 09015 TOF (e.g. by selfings and/or crossing
and/or backcrossing with NUN 09015 TOF and/or progeny thereof)
comprising all the physiological and morphological characteristics
of NUN 09015 TOF listed in Table 1 as determined at the 5%
significance level when grown under the same environmental
conditions and/or comprising one or more (or all; or all except
one, two or three) of the distinguishing characteristics as
determined at the 5% significance level when grown under the same
environmental conditions.
[0109] Also at-harvest and/or post-harvest characteristics of
fruits can be compared, such as cold storage holding quality
(browning), post-harvest rind firmness and/or flesh firmness, can
be measured using known methods.
[0110] Flesh firmness can for example be measured using a
penetrometer, e.g. by inserting a probe into the fruit flesh and
determining the insertion force, or by other methods.
[0111] The morphological and/or physiological characteristics may
vary somewhat with variation in the environment (such as
temperature, light intensity, day length, humidity, soil,
fertilizer use), which is why a comparison under the same
environmental conditions is preferred. Colors can best be measured
against The Munsell Book of Color (Munsell Color Macbeth Division
of Kollmorgan Instruments Corporation) or using the Royal
Horticultural Society Chart (World wide web at
rhs.org.uk/Plants/RHS-Publications/RHS-colour-charts).
[0112] In a preferred embodiment, the invention provides for tomato
fruits of variety NUN 09015 TOF, or a part of the fruit. In another
embodiment, the invention provides for a container comprising or
consisting of a plurality of harvested tomato fruits of NUN 09015
TOF, or progeny thereof, or a derived variety.
[0113] In yet a further embodiment, the invention provides for a
method of producing a new tomato plant. The method comprises
crossing a plant of the invention NUN 09015 TOF, or a plant
comprising all but 1, 2, or 3 of the morphological and
physiological characteristics of NUN 09015 TOF (as listed in Table
1), or a progeny plant thereof, either as male or as female parent,
with a second tomato plant (or a wild relative of tomato) one or
more times, and/or selfing a tomato plant according to the
invention i.e. NUN 09015 TOF, or a progeny plant thereof, one or
more times, and selecting progeny from said crossing and/or
selfing. The second tomato plant may for example be a line or
variety of the species Solanum Lycopersicon, S. chilense, S.
habrochaites, S. penelli, S. peruvianum S. pimpinellifolium or
other Solanum species.
[0114] Progeny are either the generation (seeds) produced from the
first cross (F1) or selfing (S1), or any further generation
produced by crossing and/or selfing (F2, F3, etc.) and/or
backcrossing (BC1, BC2, etc.) one or more selected plants of the F1
and/or S1 and/or BC1 generation (or plants of any further
generation, e.g. the F2) with another tomato plant (and/or with a
wild relative of tomato). Progeny may have all the physiological
and morphological characteristics of tomato variety NUN 09015 TOF
when grown under the same environmental conditions and/or progeny
may have (be selected for having) one or more of the distinguishing
characteristics of tomato of the invention. Using common breeding
methods such as backcrossing or recurrent selection, one or more
specific characteristics may be introduced into NUN 09015 TOF, to
provide or a plant comprising all but 1, 2, or 3 or more of the
morphological and physiological characteristics of NUN 09015 TOF
(as listed in Table 1).
[0115] The invention provides for methods of producing plants which
retain all the morphological and physiological characteristics of
NUN 09015 TOF. The invention provides also for methods of producing
a plant comprising all but 1, 2, or 3 or more of the morphological
and physiological characteristics of NUN 09015 TOF (e.g. as listed
in Table 1), but which are still genetically closely related to NUN
09015 TOF. The relatedness can, for example be determined by
fingerprinting techniques (e.g., making use of isozyme markers
and/or molecular markers such as SNP markers, AFLP markers,
microsatellites, minisatellites, RAPD markers, RFLP markers and
others). A plant is "closely related" to NUN 09015 TOF if its DNA
fingerprint is at least 80%, 90%, 95% or 98% identical to the
fingerprint of NUN 09015 TOF. In a preferred embodiment AFLP
markers are used for DNA fingerprinting (Vos et al. 1995, Nucleic
Acid Research 23: 4407-4414). A closely related plant may have a
Jaccard's Similarity index of at least about 0.8, preferably at
least about 0.9, 0.95, 0.98 or more (Ince et al., (2010) Biochem.
Genet. 48:83-95). The invention also provides plants and varieties
obtained by these methods. Plants may be produced by crossing
and/or selfing, or alternatively, a plant may simply be identified
and selected amongst NUN 09015 TOF plants, or progeny thereof, e.g.
by identifying a variant within NUN 09015 TOF or progeny thereof
(e.g. produced by selfing) which variant differs from NUN 09015 TOF
in one, two or three of the morphological and/or physiological
characteristics (e.g. in one, two or three distinguishing
characteristics), e.g. those listed in Table 1 or others.
[0116] By crossing and/or selfing also (one or more) single traits
may be introduced into the variety of the invention i.e. NUN 09015
TOF (e.g., using backcrossing breeding schemes), while retaining
the remaining morphological and physiological characteristics of
NUN 09015 TOF and/or while retaining one or more distinguishing
characteristics. A single trait converted plant may thereby be
produced. For example, disease resistance genes may be introduced,
genes responsible for one or more quality traits, yield, etc. Both
single genes (dominant or recessive) and one or more QTLs
(quantitative trait loci) may be transferred into NUN 09015 TOF by
breeding with NUN 09015 TOF.
[0117] Any pest or disease resistance genes may be introduced into
a plant according to the invention, i.e. NUN 09015 TOF, progeny
thereof or into a plant comprising all but 1, 2, or 3 or more of
the morphological and physiological characteristics of NUN 09015
TOF (e.g. as listed in Table 1). Resistance to one or more of the
following diseases is preferably introduced into plants of the
invention: Cucumber Mosaic Virus, Curly Top Virus, Tomato Mottle
Virus, Potato Y Virus, Blotchey Ripening, Tobacco Etch Virus, the
various Tobacco Mosaic Virus races, Concentric cracking, Tomato
spotted wilt, Tomato yellows, Gold Fleck, Bacterial canker,
Bacterial soft rot, Bacterial speck, Bacterial wilt, Anthracnose
(Gloeosporium piperatum), Fusarium wilt (F. oxysporum races),
Alternaria, Bacterial Spot (Xanthomonas vesicatoria), Nematode
(Meloidogyne incognita acrita), Late blight (Phytophthora infestans
races), Leaf mold (Cladosporium fulvum races), Colorado potato
beetle, Spider mites, Whitefly and Verticillium Wilt (Verticillium
dahliae).
[0118] Other resistance genes, against pathogenic viruses, fungi,
bacteria, nematodes, insects or other pests may also be
introduced.
[0119] Thus, invention also provides a method for developing a
tomato plant in a tomato breeding program, using a tomato plant of
the invention, or its parts as a source of plant breeding material.
Suitable plant breeding techniques are recurrent selection,
backcrossing, pedigree breeding, mass selection, mutation breeding
and/or genetic marker enhanced selection. For example, in one
aspect, the method comprises crossing NUN 09015 TOF or progeny
thereof, or a plant comprising all but 1, 2, or 3 or more of the
morphological and physiological characteristics of NUN 09015 TOF
(e.g. as listed in Table 1), with a different tomato plant, and
wherein one or more offspring of the crossing are subject to one or
more plant breeding techniques selected from the group consisting
of recurrent selection, backcrossing, pedigree breeding, mass
selection, mutation breeding and genetic marker enhanced selection
(see e.g. Vidaysky and Czosnek, (1998) Phytopathology 88(9):
910-4). For breeding methods in general see Principles of Plant
Genetics and Breeding, 2007, George Acquaah, Blackwell Publishing,
ISBN-13: 978-1-4051-3646-4.
[0120] The invention thus also provides a method of introducing a
single locus conversion, or single trait conversion or introducing
a desired trait, into a tomato plant according to the invention
and/or into NUN 09015 TOF comprising: [0121] (a) crossing a tomato
plant of variety NUN 09015 TOF, a representative sample of seed of
said variety having been deposited under Accession Number NCIMB
______, with a second tomato plant comprising a desired single
locus to produce F1 progeny plants; [0122] (b) selecting F1 progeny
plants that have the single locus to produce selected F1 progeny
plants; [0123] (c) crossing the selected progeny plants with a
plant of NUN 09015 TOF, to produce backcross progeny plants; [0124]
(d) selecting backcross progeny plants that have the single locus
and one or more (or all) distinguishing characteristics of tomato
according to the invention and/or all the physiological and
morphological characteristics of NUN 09015 TOF to produce selected
backcross progeny plants; and [0125] (e) optionally repeating steps
(c) and (d) one or more times in succession to produce selected
second, third or fourth or higher backcross progeny plants that
comprise the single locus and otherwise one or more (or all) the
distinguishing characteristics of the tomatos according to the
invention and/or comprise all of the physiological and
morphological characteristics of NUN 09015 TOF, when grown in the
same environmental conditions. The invention further relates to
plants obtained by this method.
[0126] The above method is provided, wherein the single locus
conversion concerns a trait, wherein the trait is pest resistance
or disease resistance.
[0127] In one embodiment the trait is disease resistance and the
resistance is conferred to Cucumber Mosaic Virus, Curly Top Virus,
Tomato Mottle Virus, Potato Y Virus, Blotchey Ripening, Tobacco
Etch Virus, the various Tobacco Mosaic Virus races, Concentric
cracking, Tomato spotted wilt, Tomato yellows, Gold Fleck,
Bacterial canker, Bacterial soft rot, Bacterial speck, Bacterial
wilt, Anthracnose (Gloeosporium piperatum), Fusarium wilt (F.
oxysporum races), Alternaria, Bacterial Spot (Xanthomonas
vesicatoria), Nematode (Meloidogyne incognita acrita), Late blight
(Phytophthora infestans races), Leaf mold (Cladosporium fulvum
races), Colorado potato beetle, Spider mites, Whitefly and
Verticillium Wilt (Verticillium dahliae).
[0128] The invention also provides a tomato plant comprising at
least a first set of the chromosomes of tomato variety NUN 09015
TOF, a sample of seed of said variety having been deposited under
Accession Number NCIMB ______; optionally further comprising a
single locus conversion, wherein said plant has essentially all of
the morphological and physiological characteristics of the plant
comprising at least a first set of the chromosomes of tomato NUN
09015 TOF. In another embodiment, this single locus conversion
confers a trait selected from the group consisting of male
sterility, herbicide tolerance, insect resistance, pest resistance,
disease resistance, environmental stress tolerance, modified
carbohydrate metabolism and modified protein metabolism.
[0129] In one embodiment, NUN 09015 TOF may also be mutated (by
e.g. irradiation, chemical mutagenesis, heat treatment, etc.) and
mutated seeds or plants may be selected in order to change one or
more characteristics of NUN 09015 TOF. Methods such as TILLING may
be applied to tomato populations in order to identify mutants.
Similarly, NUN 09015 TOF may be transformed and regenerated,
whereby one or more chimeric genes are introduced into the variety
or into a plant comprising all but 1, 2, 3, or more of the
morphological and physiological characteristics (e.g. as listed in
Table 1). Transformation can be carried out using standard methods,
such as Agrobacterium tumefaciens mediated transformation or
biolistics, followed by selection of the transformed cells and
regeneration into plants. A desired trait (e.g. genes conferring
pest or disease resistance, herbicide, fungicide or insecticide
tolerance, etc.) can be introduced into NUN 09015 TOF, or progeny
thereof, by transforming NUN 09015 TOF or progeny thereof with a
transgene that confers the desired trait, wherein the transformed
plant retains all the phenotypic and/or morphological and/or
physiological characteristics of NUN 09015 TOF or the progeny
thereof and contains the desired trait.
[0130] The invention also provides for progeny of tomato variety
NUN 09015 TOF obtained by further breeding with NUN 09015 TOF. In
one aspect progeny are F1 progeny obtained by crossing NUN 09015
TOF with another plant or S1 progeny obtained by selfing NUN 09015
TOF. Also encompassed are F2 progeny obtained by selfing the F1
plants. "Further breeding" encompasses traditional breeding (e.g.,
selfing, crossing, backcrossing), marker assisted breeding, and/or
mutation breeding. In one embodiment, the progeny have one or more
(or all) of the distinguishing characteristics mentioned further
above when grown under the same environmental conditions. In a
further embodiment the progeny have all the physiological and
morphological characteristics of variety NUN 09015 TOF when grown
under the same environmental conditions. In another embodiment the
progeny have one, two, or three distinct traits (qualitative or
quantitative) introduced into NUN 09015 TOF, while retaining all
the other physiological and morphological characteristics of
variety NUN 09015 TOF when grown under the same environmental
conditions.
[0131] An EDV is a plant having one, two or three physiological
and/or morphological characteristics which are different from those
of NUN 09015 TOF and which otherwise has all the physiological and
morphological characteristics of NUN 09015 TOF, wherein a
representative sample of seed of variety NUN 09015 TOF has been
deposited under Accession Number NCIMB ______. In particular
variants which differ from NUN 09015 TOF in none, one, two or three
of the characteristics mentioned in Table 1 are encompassed.
[0132] In one aspect, the EDV differs from NUN 09015 TOF in one,
two or three of the distinguishing morphological and/or
physiological characteristics selected from 1) average length of
mature fruit (along stem axis); 2 average diameter of mature fruit
(at widest point); 3) average weight of mature fruit; 4) average
number of locules; and 5) average number of flowers in
inflorescence.
[0133] In another embodiment the EDV may differ from NUN 09015 TOF
in one, two or three morphological or physiological characteristic
other than the "distinguishing morphological and/or physiological
characteristics" (or essential physiological and/or morphological
characteristics) of NUN 09015 TOF selected from: 6) average number
of nodes between early inflorescence; 7) typical fruit shape of
type 7; 8) an average length of dedicel (from joint to calyx
attachment); 9) pubescence on younger stems and 10) shape of
blossom end.
[0134] Tomatos according to the invention, such as the variety NUN
09015 TOF, or its progeny, or a plant having all physiological
and/or morphological characteristics but one, two or three which
are different from those of NUN 09015 TOF, can also be reproduced
using vegetative reproduction methods. Therefore, the invention
provides for a method of producing plants, or a part thereof, of
variety NUN 09015 TOF, comprising vegetative propagation of variety
NUN 09015 TOF. Vegetative propagation comprises regenerating a
whole plant from a plant part of variety NUN 09015 TOF (or from its
progeny or from or a plant having all physiological and/or
morphological characteristics but one, two or three, which are
different from those of NUN 09015 TOF), such as a cutting, a cell
culture or a tissue culture.
[0135] The invention also concerns methods of vegetatively
propagating a plant of the invention. In certain embodiments, the
method comprises the steps of: (a) collecting tissue or cells
capable of being propagated from a plant of the invention; (b)
cultivating said tissue or cells to obtain proliferated shoots; and
(c) rooting said proliferated shoots, to obtain rooted plantlets.
Steps (b) and (c) may also be reversed, i.e. first cultivating said
tissue to obtain roots and then cultivating the tissue to obtain
shoots, thereby obtaining rooted plantlets. The rooted plantlets
may then be further grown, to obtain plants. In one embodiment, the
method further comprises step (d) growing plants from said rooted
plantlets
[0136] The invention also provides for a vegetatively propagated
plant of variety NUN 09015 TOF (or from its progeny or from or a
plant having all but one, two or three physiological and/or
morphological characteristics which are different from those of NUN
09015 TOF, or a part thereof, having one or more distinguishing
characteristics and/or all the morphological and physiological
characteristics of NUN 09015 TOF (except for the characteristics
differing), when grown under the same environmental conditions.
[0137] Parts of NUN 09015 TOF (or of its progeny or of a plant
having all physiological and/or morphological characteristics but
one, two or three which are different from those of NUN 09015 TOF)
encompass any cells, tissues, organs obtainable from the seedlings
or plants, such as but not limited to: tomato fruits or parts
thereof, cuttings, hypocotyl, cotyledon, pollen and the like. Such
parts can be stored and/or processed further. Encompassed are
therefore also food or feed products comprising one or more of such
parts, such as canned, chopped, cooked, roasted, in a sauce, in a
sandwich, pasted, pureed or concentrated, juiced, frozen, dried,
pickled, or powdered tomato fruit from NUN 09015 TOF or from
progeny thereof, or from a derived variety, such as a plant having
all but one, two or three physiological and/or morphological
characteristics which are different from those of NUN 09015
TOF.
[0138] In one aspect haploid plants and/or double haploid plants of
NUN 09015 TOF, or a plant having all but one, two or three
physiological and/or morphological characteristics which are
different from those of NUN 09015 TOF, or progeny of any of these,
are encompassed herein. Haploid and double haploid (DH) plants can,
for example, be produced by cell or tissue culture and chromosome
doubling agents and regeneration into a whole plant. For DH
production chromosome doubling may be induced using known methods,
such as colchicine treatment or the like.
[0139] Also provided are plant parts derived from variety NUN 09015
TOF (or from its progeny or from a plant having all but one, two or
three physiological and/or morphological characteristics which are
different from those of NUN 09015 TOF D), or from a vegetatively
propagated plant of NUN 09015 TOF (or from its progeny or from a
plant having all but one, two or three physiological and/or
morphological characteristics which are different from those of NUN
09015 TOF), being selected from the group consisting of: harvested
fruits or parts thereof, pollen, cells, leaves or parts thereof,
petioles, cotyledons, hypocotyls, shoots or parts thereof, stems or
parts thereof, roots or parts thereof, cuttings, or flowers.
[0140] In one embodiment, the invention provides for extracts of a
plant described herein and compositions comprising or consisting of
such extracts. In a preferred embodiment, the extract consists of
or comprises tissue of a plant described herein or is obtained from
such tissue.
[0141] In still yet another aspect, the invention provides a method
of determining the genotype of a plant of the invention comprising
detecting in the genome (e.g., a sample of nucleic acids) of the
plant at least a first polymorphism. The method may, in certain
embodiments, comprise detecting a plurality of polymorphisms in the
genome of the plant, for example by obtaining a sample of nucleic
acid from a plant and detecting in said nucleic acids a plurality
of polymorphisms. The method may further comprise storing the
results of the step of detecting the plurality of polymorphisms on
a computer readable medium
[0142] The invention also provides for a food or feed product
comprising or consisting of a plant part described herein wherein
the plant part can be identified as a part of the plant of the
invention. Preferably, the plant part is a tomato fruit or part
thereof and/or an extract from a fruit or another plant part
described herein. The food or feed product may be fresh or
processed, e.g., dried, grinded, powdered, pickled, chopped,
cooked, roasted, in a sauce, in a sandwich, pasted, pureed or
concentrated, juiced, pickled, canned, steamed, boiled, fried,
blanched and/or frozen, etc.
[0143] For example, containers such as cans, boxes, crates, bags,
cartons, Modified Atmosphere Packagings, films (e.g. biodegradable
films), etc. comprising plant parts of plants (fresh and/or
processed) described herein are also provided herein.
[0144] Marketable tomato fruits are generally sorted by size and
quality after harvest. Alternatively the tomato fruits can be
sorted by expected shelf life. pH of Brix.
[0145] Tomatos may also be grown for use in grafting or
inosculation as rootstocks (stocks) or scions (cions). Typically,
different types of tomatos are grafted to enhance disease
resistance, which is usually conferred by the rootstock, while
retaining the horticultural qualities usually conferred by the
scion. It is not uncommon for grafting to occur between cultivated
tomato varieties and related Solanum species. Methods of grafting
and vegetative propagation are well-known in the art.
[0146] So in one aspect the invention relates to a plant comprising
a rootstock or scion of NUN 09015 TOF.
[0147] Using methods known in the art like "reverse breeding", it
is possible to produce parental lines for a hybrid plant such as
NUN 09015 TOF; where normally the hybrid is produced from the
parental lines. Such methods are based on the segregation of
individual alleles in the spores produced by a desired plant and/or
in the progeny derived from the self-pollination of that desired
plant, and on the subsequent identification of suitable progeny
plants in one generation, or in a limited number of inbred cycles.
Such a method is known from WO2014076249 or from Nature Protocols
Volume: 9, Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049,
which are enclosed by reference. Such method for producing parental
lines for a hybrid organism, comprises the steps of: a) defining a
set of genetic markers that are present in a heterozygous form (H)
in a partially heterozygous starting organism; b) producing doubled
haploid lines from spores of the starting organism: c) genetically
characterizing the doubled haploid lines thus obtained for the said
set of genetic markers to determine whether they are present in a
first homozygous form (A) or in a second homozygous form (B); d)
selecting at least one pair of doubled haploid lines that have
complementary alleles for at least a subset of the genetic markers,
wherein each member of the pair is suitable as a parental line for
a hybrid organism.
[0148] Thus in one aspect, the invention relates to a method of
producing a combination of parental lines of a plant of the
invention (NUN 09015 TOF) comprising the step of making double
haploid cells from haploid cells from the plant of the invention
(NUN 09015 TOF) or a seed of that plant; and optionally crossing
these parental lines to produce and collect seeds. In another
aspect, the invention relates to a combination of parental lines
produced by this method. In still another aspect said combination
of parental lines can be used to produce a seed or plant of NUN
09015 TOF when these parental lines are crossed. In still another
aspect, the invention relates to a combination of parental lines
from which a seed or plant having all but one, two or three
physiological and/or morphological characteristics which are
different from those of NUN 09015 TOF can be produced or in another
aspect, wherein a seed or plant having the distinguishing
characteristics 1)-5) or 1)-10) of NUN 09015 TOF, as herein
defined, can be produced when grown under the same environmental
conditions. In still another aspect, the invention relates to a
combination of parental lines from which a seed or plant having all
the characteristics of NUN 09015 TOF as defined in Table 1 can be
produced when grown under the same conditions.
[0149] All documents (e.g., patent publications) are herein
incorporated by reference in their entirety. Cited references:
[0150] In one embodiment of the invention, the first step in
"crossing" comprises planting seeds of a first and a second parent
tomato plant, often in proximity so that pollination will occur for
example, mediated by insect vectors. Alternatively, pollen can be
transferred manually. Where the plant is self-pollinated,
pollination may occur without the need for direct human
intervention other than plant cultivation.
Development of NUN 09015 TOF
[0151] The hybrid NUN 09015 TOF was developed from a male and
female proprietary inbred line of Nunhems. The female and male
parents were crossed to produce hybrid (F1) seeds of NUN 09015 TOF
The seeds of NUN 09015 TOF can be grown to produce hybrid plants
and parts thereof (e.g. pepper fruit). The hybrid NUN 09015 TOF can
be propagated by seeds or vegetative.
[0152] The hybrid variety is uniform and genetically stable. This
has been established through evaluation of horticultural
characteristics. Several hybrid seed production events resulted in
no observable deviation in genetic stability. Coupled with the
confirmation of genetic stability of the female and male parents
the Applicant concluded that NUN 09015 TOF is uniform and
stable.
[0153] DANIELA is considered to be the most similar variety to NUN
09015 TOF. DANIELA is a commercial variety from Sakata. In Table 1
a comparison between NUN 09015 TOF and DANIELA is shown based on a
greenhouse trial in the USA. Trial location I: Acampo, Calif., USA
(coordinates: 38.degree.192873''N 121.degree.232637''W). Seeding
date: Apr. 3, 2014. Transplanting date: May 12, 2014. Harvest date:
July, 2014.
[0154] Two replications of 50 plants each, from which 15 plants or
plant parts were randomly selected, were used to measure
characteristics. In Table 1 the USDA descriptors of NUN 09015 TOF
(this application) and reference DANIELA (commercial variety) are
listed.
[0155] In accordance with one aspect of the present invention,
there is provided a plant having the physiological and
morphological characteristics of tomato variety NUN 09015 TOF. A
description of the physiological and morphological characteristics
of tomato variety NUN 09015 TOF is presented in Table 1.
TABLE-US-00001 TABLE 1 Objective description of varieties NUN 09015
TOF and DANIELA NUN 09015 TOF DANIELA 1. Seedling Anthocyanin in
hypocotyl 2 2 1 = absent, 2 = present habit of 3-4 week old
seedling 1 1 1 = normal, 2 = compact 2. Mature plant Height NA NA
Growth type 1 1 1 = indeterminate; 2 = determinate Form 2 2 1 =
lax; 2 = normal; 3 = compact; 4 = dwarf; 5 = brachytic Size of
canopy (compared to similar form) 2 2 1 = small; 2 = medium; 3 =
large Habit 1 1 1 = sprawling; 2 = semi-erect; 3 = erect (Dwarf
Champion) 3. Stem Branching 2 2 1 = sparse (Brehm's Solid Red;
Fireball); 2 = intermediate (Westover); 3 = profuse (UC 82)
Branching at cotyledon or first leafy node 2 2 1 = present; 2 =
absent Number of nodes before first inflorescence 3 3 Number of
nodes between early (1.sup.st to 2.sup.nd, 2.sup.nd 2 2 to
3.sup.rd) inflorescence Number of nodes between later developing NA
NA inflorescences Pubescence on younger stems 3 2 1 = smooth (no
long hairs); 2 = sparsely hairy (scattered long hairs); 3 =
moderately hairy; 4 = densely hairy or wooly 4. Leaf Type: 1 1 1 =
tomato; 2 = potato (Trip-L-Crop) Morphology (corresponding to
Exhibit C 2 2 illustration) Margins of major leaflets 2 2 1 =
absent; 2 = shallowly toothed or scalloped; 3 = deeply toothed or
cut, sps. towards base Marginal rolling or wiltiness 1 1 1 =
absent; 2 = slight; 3 = moderate; 4 = strong Onset of leaflet
rolling NA NA 1 = early-season; 2 = mid-season; 3 = late-season
Surface of major leaflets 2 2 1 = smooth; 2 = rogues (bumpy or
veiny) Pubescence 2 2 1 = smooth (no long hairs); 2 = normal; 3 =
hirsute; 4 = wooly 5. Inflorescence Type 1 1 1 = simple, 2 =
forked, 3 = compound Number of flowers in inflorescence 7.7 5.87
Leafy or "running" inflorescences 1 2 1 = absent, 2 = occasional, 3
= frequent 6. Flower Calyx 1 1 1 = normal, lobes awl-shaped; 2 =
macrocalyx, lobes large, leaflike; 3 = fleshy Calyx-lobes NA 1 1 =
shorter the corolla; 2 = approx., equaling corolla; 3 = distinctly
longer than corolla Corolla color 1 1 1 = yellow: 2 = old gold; 3 =
white or tan Style pubescence 2 2 1 = absent; 2 = sparse; 3 = dense
Anthers 1 1 1 = all fused into tube; 2 = separating into 2 or more
groups at anthesis Fasciation (1st flower of 2.sup.nd or 3.sup.rd
inflorescence) 1 1 1 = absent; 2 = occasionally present; 3 =
frequently present 7. fruit Typical shape (corresponding to Exhibit
C 7 2 illustration) Shape of transverse section 1 1 1 = round; 2 =
flattened; 3 = angular; 4 = irregular Shape of stem end 1 1 1 =
flat; 2 = indented Shape of blossom end 3 3 1 = indented; 2 = flat;
3 = nippled; 4 = tapered Shape of pistil scar 1 1 1 = dot; 2 =
stellate; 3 = linear; 4 = irregular Abscission layer 1 1 1 =
present (pedicellate); 2 = absent (jointless) Point of detachment
of fruit at harvest 1 1 1 = at pedicel joint; 2 = at calyx
attachment Length of dedicel (from joint to calyx attachment) 15.6
mm 13.1 Length of mature fruit (stem axis) 62 mm 48.9 Diameter of
fruit at widest point 47 mm 57.8 Weight of mature fruit 71.3 mm
92.47 Number of locules 1 2 1 = two; 2 = three or four; 3 = five or
more Fruit surface 1 1 1 = smooth; 2 = slightly rough; 3 =
moderately rough or ribbed Fruit base color (mature-green stage) 3
3 1 = light green (Lanal; VF 145-F5); 2 = light gray-green; 3 =
apple or medium green (Heinz 1439 VF); 4 = yellow green; 5 = dark
green Fruit pattern (mature-green stage) 1 2, 1 = uniform green; 2
= green-shouldered; 3 = radial stripes on sides of fruit Shoulder
color if different from base NA 1 1 = dark green; 2 = grey green; 3
= yellow green Fruit color full ripe: 5 5 1 = white; 2 = yellow; 3
= orange; 4 = pink; 5 = red; 6 = brownish 7 = greenish; 8 = other
Flesh color full ripe: 3 3 1 = yellow; 2 = pink; 3 = red/crimson; 4
= orange; 5 other Flesh color: 1 1 1 = uniform; 2 = with lighter
and darker areas in walls Locular gel color of table-ripe fruit 3 3
1 = green; 2 = yellow; 3 = red Ripening 2 2 1 = blossom to stem
end; 2 = uniform Ripening 2 2 1 = inside out; 2 = uniformity; 3 =
outside in Stem scar size: 1 1 1 = small (Roma); 2 = medium
(Rutgers); 3 = large Core: 1 1 1 = coreless (absent or smaller than
6 .times. 6 mm); 2 = present Epidermis color: 2 2 1 = colorless; 2
= yellow Epidermis: 1 1 1 = normal; 2 = easy-peel Epidermis
texture: 2 2 1 = tender; 2 = average; 3 = tough Thickness of
pericarp: 7.7 mm 7.3 mm 9. Disease and Pest Reaction NA NA 1 highly
resistant, 2 intermediate resistance, 3 susceptible, 4 not
determined 10. Chemistry and composition of full-ripe NA NA fruits
11. Phenology NA NA 12. Adaptation Culture 2 2, 1 1 = field, 2 =
greenhouse Principle use 2 2 5 = other (specify) Regions to which
adaptation has been 9 9 demonstrated: 1 = Northeast; 2 = Mid
Atlantic; 3 = Southeast; 4 Florida; 5 = Great Plains, 6 = south
central; 7 = Intermountain West; 8 = Northwest; 9 = California
(Sacramento and Upper San Joaquin Valley); 10 = California (Coastal
Areas); 11 California (Southern San Joaquin Valley &
desserts)
[0156] Table 1 contains typical values. Values may vary due to
environment. Other values that are substantially equivalent are
also within the scope of the invention. N.A.=not applicable;
n.r.=not recorded.
Deposit Information
[0157] A total of 2500 seeds of the hybrid variety NUN 09015 TOF
were deposited according to the Budapest Treaty by Nunhems B.V. on
______, at or at the NCIMB Ltd., Ferguson Building, Craibstone
Estate, Bucksburn, Aberdeen AB21 9YA, United Kingdom (NCIMB). The
deposit has been assigned or NCIMB ______. A deposit of NUN NUN
09015 TOF and of the male and female parent line is also maintained
at Nunhems B.V. Access to the deposit will be available during the
pendency of this application to persons determined by the Director
of the U.S. Patent Office to be entitled thereto upon request.
Subject to 37 C.F.R. .sctn.1.808(b), all restrictions imposed by
the depositor on the availability to the public of the deposited
material will be irrevocably removed upon the granting of the
patent. The deposit will be maintained for a period of 30 years, or
5 years after the most recent request, or for the enforceable life
of the patent whichever is longer, and will be replaced if it ever
becomes nonviable during that period. Applicant does not waive any
rights granted under this patent on this application or under the
Plant Variety Protection Act (7 USC 2321 et seq.).
* * * * *