Stress Tolerant Transgenic Crop Plants

Abstract

Disclosed herein are novel variant bacterial cold shock proteins and recombinant DNA for expressing such proteins to produce transgenic plants with enhanced stress tolerance and/or enhanced yield.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present application is a divisional application of U.S. patent application Ser. No. 13/059,116, incorporated herein by reference in its entirety, which was the National Stage application of International Patent Application No. PCT/US2009/053807, filed Aug. 14, 2009 and incorporated herein by reference in its entirety, which claims the benefit of U.S. Provisional Application Ser. No. 61/089,058 filed Aug. 15, 2008, which is incorporated herein by reference in its entirety.

INCORPORATION OF SEQUENCE LISTING

[0002] The sequence listing that is contained in the file named "seqlisting.sub.--55486_US.sub.--0002 .txt-", which is 501199 bytes (measured in operating system MS-Windows), created on Aug. 30, 2014, is filed herewith by electronic submission and incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

[0003] Disclosed herein are transgenic plant cells in seeds and plants with improved stress tolerance and methods of making and using such cells, seeds and plants.

BACKGROUND OF THE INVENTION

[0004] Transgenic plants comprising recombinant DNA for expression of a cold shock protein have been demonstrated to have improved abiotic stress tolerance (International Patent Application WO05033318).

SUMMARY OF THE INVENTION

[0005] This invention provides novel proteins derived from bacterial cold shock proteins, which upon expression in transgenic plants provide the plants with enhanced stress tolerance. This invention also provides recombinant DNA constructs for expression of such polypeptides. In various aspects of the invention, a recombinant DNA construct comprises a promoter that functions in plants operably linked to nucleotides encoding a protein having a sequence of any one of SEQ ID NO:15 through SEQ ID NO:33 or SEQ ID NO:53 through SEQ ID NO:704, wherein said recombinant DNA construct is stably integrated into a chromosome in a transgenic plant cell. In various aspects of the invention, the polypeptides provide plants with improved yield as compared to control crop plants when the plants are grown under abiotic stress conditions, including high salt, heat, drought or water deficit and cold temperatures.

[0006] Also provided in the invention are transgenic plant cells having the recombinant DNA of this invention stably integrated into the plant genome and abiotic stress tolerant plants comprising such plant cells. Abiotic stress tolerant plants express the variant bacterial cold shock proteins of this invention, and include crop plants that are improved in at least one trait selected from heat tolerance, salt tolerance, drought tolerance, and survival after cold shock. The modified bacterial cold shock proteins also provide crop plants having comparable or improved yield as compared to control crop plants when grown in non-stress conditions, including conditions of moderate temperatures and sufficient water. Crop plants of particular interest in the present invention include corn, soybean, cotton, canola, alfalfa, wheat, rice, switchgrass, sugarcane, and sugar beet. This invention also provides transgenic plant cells, propagules, including seeds, and crops having the novel recombinant DNA of this invention.

[0007] In another aspect of the invention a plant chromosome having stably integrated DNA for expression of a variant bacterial cold shock protein is provided. Transgenic pollen grains comprising a haploid derivative of a plant cell containing a plant chromosomal DNA segment of this invention are also provided. Another aspect of this invention is anti-counterfeit milled seed having, as an indication of origin, a plant cell with said chromosomal DNA segment of this invention.

[0008] The present invention is also directed to methods of plant and seed production. One method provides for the production of transgenic plants having enhanced abiotic stress tolerance by introducing (by transformation or introgressing) recombinant DNA for expression of a variant bacterial cold shock protein into plant cells to provide transgenic plant cells, regenerating a transgenic plant from one or more of said transgenic plant cells, and screening a population of transgenic plants to select a transgenic plant expressing a variant bacterial cold shock protein and having enhanced abiotic stress tolerance.

[0009] Another method of this invention provides for the manufacture of non-natural, transgenic seed or propagules that can be used to produce a crop of transgenic plants with enhanced abiotic stress tolerance resulting from expression of a variant bacterial cold shock protein from a plant chromosomal DNA segment of this invention. Such a method comprises screening a population of plants having such plant chromosomal DNA segment for said enhanced abiotic stress tolerance, selecting from said population one or more plants that exhibit enhanced yield as compared to the yield for control plants under abiotic stress conditions, verifying that said plant chromosomal DNA segment is stably integrated in said selected plants, and collecting seed or a regenerative propagule from a selected plant.

[0010] Another method provides for the production of inbred corn seed comprising acquiring hybrid corn seed from a herbicide tolerant corn plant which also has a stably-integrated, chromosomal DNA segment of this invention comprising recombinant DNA for expression of a variant bacterial cold shock protein, introgressing the chromosomal DNA segment from said acquired hybrid corn seed into a second corn line by allowing pollen grains comprising a haploid derivative with said chromosomal DNA segment to pollinate said second corn line to produce crossed seeds, producing a population of plants from crossed seeds (where a fraction of the seeds produced from said pollination is homozygous for the chromosomal DNA segment, a fraction is hemizygous, and a fraction does not have the chromosomal DNA segment), selecting corn plants which are homozygous and hemizygous for said chromosomal DNA segment by treating with an herbicide, collecting seed from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants, and backcrossing plants grown from said progeny seeds with said second corn line to produce an inbred corn line. The method can be further employed by crossing the inbred corn line with a third corn line to produce hybrid seed.

[0011] Yet another aspect of this invention provides a method of growing a corn, cotton, soybean, sugarcane, switchgrass, rice, wheat, alfalfa, or canola crop without irrigation water comprising planting seed having plant cells with a plant chromosomal DNA segment of this invention, where the seeds are produced from plants that are selected for enhanced water deficit stress tolerance.

[0012] Recombinant DNAs comprising a promoter that functions in plants operably linked to a polynucleotide encoding a protein, wherein the protein is a chimeric bacterial cold shock protein wherein at least one Reg 1, Reg 2, Reg 3, Reg 4, or Reg 5 amino acid region of a first bacterial cold shock protein is substituted with at least one corresponding Reg 1, Reg 2, Reg 3, Reg 4, or Reg 5 amino acid region of at least one second bacterial cold shock protein are provided. In certain embodiments, the first bacterial cold shock protein is selected from the group consisting of CspB, CspC, CspD, CspG, Csp1, and Csp2. In still other embodiments, the first bacterial cold shock protein is a CspB protein that comprises a CspB L2V protein. A CspB L2V protein can comprise the protein of SEQ ID NO:1. In any of the aforementioned recombinant DNAs encoding chimeric bacterial cold shock proteins, the second bacterial cold shock protein can be selected from the group consisting of a Thermotoga maritima cold shock protein, a Thermoanaerobacter tengcongensis cold shock protein, an Escherichia coli cold shock protein, and an Agrobacterium tumefaciens cold shock protein. In any of the aforementioned recombinant DNAs encoding chimeric bacterial cold shock proteins, the second bacterial cold shock protein can also be selected from the group consisting of a Thermotoga maritima Csp1 cold shock protein, a Thermoanaerobacter tengcongensis Csp1 cold shock protein, an Escherichia coli CspC cold shock protein, an Escherichia coli CspD cold shock protein, an Escherichia coli CspG cold shock protein, and an Agrobacterium tumefaciens Csp2 cold shock protein. In certain embodiments, the recombinant DNA encodes a chimeric bacterial cold shock protein that comprises a protein with an amino acid sequence selected from the group consisting of SEQ ID NO: 16 through SEQ ID NO:23, SEQ ID NO: 313 through SEQ ID NO:426, and SEQ ID NO:427.

[0013] Also provided are recombinant DNAs comprising a promoter that functions in plants operably linked to a polynucleotide encoding a protein, wherein the protein is a variant of a Bacillus subtilis cspB protein having one or more amino acid substitutions in an amino acid position corresponding to amino acids F15, F17, F27, H29, or F30 of Bacillus subtilis cspB of SEQ ID NO:1. In certain embodiments, the recombinant DNA encodes a protein that comprises a protein with an amino acid sequence selected from the group consisting of SEQ ID NO:53 through SEQ ID NO:57, SEQ ID NO:458 through SEQ ID NO:577, and SEQ ID NO:578.

[0014] Also provided are recombinant DNAs comprising a promoter that functions in plants operably linked to a polynucleotide encoding a protein, wherein the protein is a variant of a Bacillus subtilis cspB protein having one or more amino acid substitutions in an amino acid position corresponding to amino acids S31 and T40 of Bacillus subtilis cspB of SEQ ID NO:1. In certain embodiments, the recombinant DNAs encodes a protein that comprises a protein with an amino acid sequence selected from the group consisting of SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:428 through SEQ ID NO:456, and SEQ ID NO:457.

[0015] Also provided are recombinant DNAs comprising a promoter that functions in plants operably linked to a polynucleotide encoding a protein, wherein the protein is a variant of a Bacillus subtilis cspB protein having at least two amino acid residues N and N+2 replaced with alanine residues, wherein N corresponds to any one of amino acids 2-65 of SEQ ID NO:1. In certain embodiments, the protein encoded by the recombinant DNA comprises a protein with an amino acid sequence selected from the group consisting of SEQ ID NO:270 through SEQ ID NO:311, and SEQ ID NO:312.

[0016] Also provided are recombinant DNAs comprising a promoter that functions in plants operably linked to a polynucleotide encoding a protein, wherein said protein is a variant of a Bacillus subtilis cspB protein comprising a mutation in a DOT1, DOT2, DOT3, DOT4, DOT5a, DOT5b, or DOT6 region of a Bacillus subtilis CspB protein, wherein said DOT1, DOT2, DOT3, DOT4, DOT5a, DOT5b, or DOT6 region are as provided in FIG. 2. In certain embodiments, the protein encoded by the recombinant DNA comprises a protein with an amino acid sequence selected from the group consisting of SEQ ID NO:15, SEQ ID NO:24 through SEQ ID NO:31, SEQ ID NO:58 through SEQ ID NO:269, SEQ ID NO:579 through SEQ ID NO:703, and SEQ ID NO:704.

[0017] Also provided herein are any of the aforementioned recombinant DNAs, wherein the promoter is selected from the group consisting of inducible promoters, constitutive promoters, temporal-regulated promoters, developmentally-regulated promoters, tissue-preferred promoters, cold enhanced promoters, cold-specific promoters, stress enhanced promoters, stress specific promoters, drought inducible promoters, water deficit inducible promoters, and tissue-specific promoters.

[0018] Also provided herein are cells that comprises any of the aforementioned recombinant DNA molecules. In certain embodiments, the cell is selected from the group consisting of a bacterial cell, a yeast cell, an isolated mammalian cell, and a plant cell. In certain embodiments, the cell is a plant cell, wherein the plant cell is in a transgenic plant, wherein any of the aforementioned recombinant DNAs is stably integrated into a chromosome of said transgenic plant cell and wherein the recombinant DNA confers abiotic stress-tolerance. In certain embodiments where the plant cell is in a transgenic plant, the plant is selected from the group consisting of soybean, corn, canola, rice, cotton, barley, oats, alfalfa, sugarcane, turf grass, cotton, and wheat. Also provided are: i) non-natural transgenic plant comprising a plurality of plant cells comprising the aforementioned recombinant DNA molecules; and ii) a plant propagule of the transgenic plants comprising a plurality of plant cells comprising the aforementioned recombinant DNA molecules. In certain embodiments, the plant propagule is a seed.

[0019] Also provided are methods for obtaining an abiotic-stress resistant plant comprising planting of a seed of the transgenic plants comprising a plurality of plant cells comprising the aforementioned recombinant DNA molecules.

[0020] Also provided are methods of producing non-natural transgenic plant seed comprising the steps of: a) screening a population of plants for enhanced abiotic stress tolerance and the presence of any of the aforementioned recombinant DNAs, wherein individual plants in the population exhibit said abiotic stress tolerance at a level less than, essentially the same as, or greater than the level that said abiotic stress tolerance is exhibited in control plants that do not contain said recombinant DNA; b) selecting from said population one or more plants that exhibit said abiotic stress tolerance trait at a level greater than the level that said trait is exhibited in control plants; and c) collecting seed from selected plants from step b. In certain embodiments of the methods, the enhanced abiotic stress tolerance is to an abiotic stress is selected from the group consisting of heat tolerance, salt tolerance, drought tolerance, and survival after cold shock.

[0021] Also provided are proteins encoded by the polynucleotide that is operably linked to the promoter of any of the aforementioned the recombinant DNAs. In certain embodiments, this protein is in a plant cell. In still other embodiments, this protein is in a processed plant product.

[0022] Also provided herein are processed plant products comprising a detectable amount of the polynucleotide that is operably linked to the promoter of any of the aforementioned recombinant DNAs. In certain embodiments, the processed plant product comprises a feed, a meal, a flour, an extract, or a homogenate, wherein said feed, meal, flour, extract, or homogenate is obtained from at least one plant part. In certain embodiments, the processed plant product can be obtained from a plant part that is a stem, a leaf, a root, a flower, a tuber, or a seed. In certain embodiments, the processed product can comprise an extract that comprises a composition enriched for one or more protein(s), one or more monosaccharide(s), one or more disaccharide(s), one or more polysaccharides, or one or more fatty acid(s).

[0023] Also provided herein are processed plant products comprising a detectable amount of the protein encoded by the polynucleotide that is operably linked to the promoter of any of the aforementioned recombinant DNAs. In certain embodiments, the processed plant product comprises a feed, a meal, a flour, an extract, or a homogenate, wherein said feed, meal, flour, extract, or homogenate is obtained from at least one plant part. In certain embodiments, the processed plant product can be obtained from a plant part that is a stem, a leaf; a root, a flower, a tuber, or a seed. In certain embodiments, the processed product can comprise an extract that comprises a composition enriched for one or more protein(s), one or more monosaccharide(s), one or more disaccharide(s), one or more polysaccharides, or one or more fatty acid(s).

[0024] Also provided herein is a plant genomic DNA comprising a recombinant DNA molecule of any one of claims 1-14, or 15, wherein said recombinant DNA is stably integrated into a chromosome of said plant genomic DNA and wherein said recombinant DNA confers abiotic stress-tolerance to a plant comprising said chromosome.

BRIEF DESCRIPTION OF THE DRAWINGS

[0025] FIG. 1 provides an alignment of the bacterial cold shock proteins forming the basis for the proteins of the present invention. Regions used in generating domain swap variants are indicated as Reg1 through Reg5.

[0026] FIG. 2 displays regions and specific targeted amino acid substitutions for variant generation using degenerate oligonucleotides. The topmost sequence is SEQ ID NO:1.

[0027] FIG. 3 discloses a base vector for corn transformation.

[0028] FIG. 4 discloses a base vector for rice transformation.

[0029] FIG. 5 discloses a base vector for Arabidopsis transformation.

[0030] FIG. 6 discloses a base vector for soybean transformation.

[0031] FIG. 7 discloses a base vector for cotton transformation.

DETAILED DESCRIPTION OF THE INVENTION

[0032] The invention provides recombinant DNA derived from DNA encoding bacterial cold shock proteins where the recombinant DNA encodes a protein having one or more amino acid substitutions in the sequence of Bacillus subtilis CspB L2V (SEQ ID NO:1) or region swaps from among cold shock proteins from Escherichia coli (CspC (SEQ ID NO:4), CspD (SEQ ID NO:5), and CspG (SEQ ID NO:7)), Thermoanaerobacter tengcongensis (SEQ ID NO:3), Thermotoga maritime (SEQ ID NO:2), Agrobacterium tumefaciens (SEQ ID NO:6) and Bacillus subtilis (SEQ ID NO:1). DNA encoding these cold shock proteins are provided as SEQ ID NO:8 through SEQ ID NO:14.

[0033] As used herein, the phrases "bacterial cold shock protein(s)", "bacterial Csp(s))" or "bacterial CSP(s))" refers to any of: i) proteins that have at least 40% identity to any one of Bacillus subtilis CspB L2V (SEQ ID NO:1), Thermotoga maritime (SEQ ID NO:2), Thermoanaerobacter tengcongensis (SEQ ID NO:3), Escherichia coli CspC (SEQ ID NO:4), Escherichia coli CspD (SEQ ID NO:5), Agrobacterium tumefaciens (SEQ ID NO:6), or Escherichia coli CspG (SEQ ID NO:7); and/or, ii) proteins comprising the conserved cold shock domain (Prosite motif PS00352; Bucher and Bairoch, (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology, Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., pp 53-61, AAAIPress, Menlo Park, 1994; Hofmann et al., Nucleic Acids Res. 27:215, 1999). A bacterial cold shock protein, as described herein, is thus at least 40% identical, more preferably at least 50% identical, more preferably at least 60% identical, more preferably at least 70% identical, more preferably at least 80% identical, more preferably at least 90% identical, more preferably at least 95% identical, and most preferably at least 98% identical to any one of Bacillus subtilis CspB L2V (SEQ ID NO:1), Thermotoga maritime (SEQ ID NO:2), Thermoanaerobacter tengcongensis (SEQ ID NO:3), Escherichia coli CspC (SEQ ID NO:4), Escherichia coli CspD (SEQ ID NO:5), Agrobacterium tumefaciens (SEQ ID NO:6), or Escherichia coli CspG (SEQ ID NO:7). Lists of various bacterial cold shock proteins useful in the practice of this invention are provided in US Patent Application publication US 2005/0097640, which is incorporated herein by reference in its entirety.

[0034] As used herein "enhanced abiotic stress tolerance" characterizes a transgenic plant with enhanced tolerance to a high salt environment, heat exposure, drought exposure or water deficit environment or cold exposure as compared to a control plant.

[0035] As used herein "water deficit" means a period when water available to a plant is not replenished at the rate at which it is consumed by the plant. A long period of water deficit is colloquially called drought. Lack of rain or irrigation may not produce immediate water stress if there is an available reservoir of ground water for the growth rate of plants. Plants grown in soil with ample groundwater can survive days without rain or irrigation without adverse affects on yield. Plants grown in dry soil are likely to suffer adverse affects with minimal periods of water deficit. Severe water deficit stress can cause wilt and plant death; moderate drought can cause reduced yield, stunted growth or retarded development. Plants can recover from some periods of water deficit stress without significantly affecting yield. However, water deficit stress at the time of pollination can have an irreversible effect in lowering yield. Thus, a useful period in the life cycle of corn for observing water deficit stress tolerance is the late vegetative stage of growth before tasseling. Water deficit stress tolerance is determined by comparison to control plants. For instance, plants of this invention can survive water deficit stress with a higher yield than control plants. In the laboratory and in field trials drought can be simulated by giving plants of this invention and control plants less water than is given to sufficiently-watered control plants and measuring differences in traits.

[0036] A "control plant" may be a non-transgenic plant of the parental line used to generate a transgenic plant herein. A control plant may in some cases be a transgenic plant line that includes an empty vector or marker gene, but does not contain the recombinant polynucleotide of the present invention that is expressed in the transgenic plant being evaluated. A control plant in other cases is a transgenic plant expressing the gene with a constitutive promoter. In general, a control plant is a plant of the same line or variety as the transgenic plant being tested, lacking the specific trait-conferring, recombinant DNA that characterizes the transgenic plant. Such a progenitor plant that lacks that specific trait-conferring recombinant DNA can be a natural, wild-type plant, an elite, non-transgenic plant, or a transgenic plant without the specific trait-conferring, recombinant DNA that characterizes the transgenic plant. The progenitor plant lacking the specific, trait-conferring recombinant DNA can be a sibling of a transgenic plant having the specific, trait-conferring recombinant DNA. Such a progenitor sibling plant may include other recombinant DNA.

[0037] In an important aspect of the invention the transgenic plants have enhanced yield, including increased yield under abiotic stress conditions and increased yield under non-stress conditions. "Yield" can be affected by many properties including without limitation, plant height, pod number, pod position on the plant, number of internodes, incidence of pod shatter, grain size, efficiency of nodulation and nitrogen fixation, efficiency of nutrient assimilation, resistance to biotic and abiotic stress, carbon assimilation, plant architecture, resistance to lodging, percent seed germination, seedling vigor, and juvenile traits. Yield can also be affected by efficiency of germination (including germination in stressed conditions), growth rate (including growth rate in stressed conditions), ear number, seed number per ear, seed size, composition of seed (starch, oil, protein) and characteristics of seed fill.

[0038] Increased yield of a transgenic plant of the present invention can be measured in a number of ways, including test weight, seed number per plant, seed weight, seed number per unit area (i.e. seeds, or weight of seeds, per acre), bushels per acre, tons per acre, or kilo per hectare. For example, corn yield may be measured as production of shelled corn kernels per unit of production area, for example in bushels per acre or metric tons per hectare, often reported on a moisture adjusted basis, for example at 15.5 percent moisture. Moreover a bushel of corn is defined by law in the State of Iowa as 56 pounds by weight, a useful conversion factor for corn yield is: 100 bushels per acre is equivalent to 6.272 metric tons per hectare. Other measurements for yield are in common practice.

[0039] A transgenic "plant cell" means a plant cell that is transformed with stably-integrated, non-natural, recombinant polynucleotides, e.g. by Agrobacterium-mediated transformation or by bombardment using microparticles coated with recombinant polynucleotides. A plant cell of this invention can be an originally-transformed plant cell that exists as a microorganism or as a progeny plant cell that is regenerated into differentiated tissue, e.g. into a transgenic plant with stably-integrated, non-natural recombinant polynucleotides in its chromosomal DNA, or seed or pollen derived from a progeny transgenic plant.

[0040] A "transgenic" plant or seed means one whose genome has been altered by the stable integration of recombinant polynucleotides in its chromosomal DNA, e.g. by transformation, by regeneration from a transformed plant from seed or propagule or by breeding with a transformed plant. Thus, transgenic plants include progeny plants of an original plant derived from a transformation process including progeny of breeding transgenic plants with wild type plants or other transgenic plants. The enhancement of a desired trait can be measured by comparing the trait property in a transgenic plant which has recombinant DNA conferring the trait to the trait level in a progenitor plant. Although many varieties of plants can be advantageously transformed with recombinant DNA for expressing a variant bacterial cold shock protein to provide stress tolerance and/or enhanced yield, especially useful abiotic stress tolerant transgenic plants include corn (maize), soybean, cotton, canola (rape), wheat, rice, alfalfa, sorghum, grasses such as switchgrass, vegetables and fruits.

[0041] "Expressing a protein" means the function of a cell to transcribe recombinant DNA to mRNA and translate the mRNA to a protein. For expression the recombinant DNA usually includes regulatory elements including 5' regulatory elements such as promoters, enhancers, and introns; other elements can include polyadenylation sites, transit peptide DNA, markers and other elements commonly used by those skilled in the art. Promoters can be modulated by proteins such as transcription factors and by intron or enhancer elements linked to the promoter. Promoters in recombinant polynucleotides can also be modulated by nearby promoters.

[0042] "Recombinant DNA" means non-natural DNA, that has been prepared by modifying natural DNA or by combining segments of DNA, e.g., by chemical synthesis or by the manipulation of isolated segments of nucleic acids by genetic engineering techniques. Recombinant DNA for expressing a protein in a plant is typically provided as an expression cassette which has a promoter that is active in plant cells operably linked to DNA encoding a protein, e.g. a variant bacterial cold shock protein, linked to a 3' DNA element for providing a polyadenylation site and signal. Useful recombinant DNA also includes expression cassettes for expressing one or more proteins conferring herbicide tolerance and/or insect resistance. Useful promoters for expressing the variant cold shock proteins of the present invention in transgenic plant cells include constitutive promoters such as those from rice actin, rice GOS2 (SEQ ID NO:705), and CaMV35S. Promoters for use in the present invention may include a number of regulatory elements, such as enhancers, leaders and introns. Also of interest are chimeric promoters which include regulatory elements from different sources, such as the CaMV 35S enhanced rice actin promoter provided herein as SEQ ID NO:706. These and numerous other promoters that function in plant cells are known to those skilled in the art and available for use in alternative embodiments of this invention to provide for expression of variant bacterial cold shock proteins in transgenic plant cells.

[0043] Recombinant DNA constructs also generally include a 3' element that typically contains a polyadenylation signal and site. Well-known 3' elements include those from Agrobacterium tumefaciens genes such as nos 3', tml 3', tmr 3', tms 3', ocs 3', tr7 3', e.g., disclosed in U.S. Pat. No. 6,090,627. 3' elements from plant genes such as wheat (Triticum aestivum) heat shock protein 17 (Hsp17 3'), a wheat ubiquitin gene, a wheat fructose-1,6-biphosphatase gene, a rice glutelin gene, a rice lactate dehydrogenase gene and a rice beta-tubulin gene are disclosed in U.S. published patent application 2002/0192813 A1.

[0044] Constructs and vectors may also include a transit peptide for targeting of a gene target to a plant organelle, particularly to a chloroplast, leucoplast or other plastid organelle. The use of chloroplast transit peptides is disclosed in U.S. Pat. Nos. 5,188,642 and 5,728,925.

Plant Cell Transformation Methods

[0045] Numerous methods for transforming plant cells with recombinant DNA are known in the art and may be used in the present invention. Two commonly used methods for plant transformation are Agrobacterium-mediated transformation and microprojectile bombardment. Microprojectile bombardment methods are illustrated in U.S. Pat. No. 5,015,580 (soybean); U.S. Pat. No. 5,550,318 (corn); U.S. Pat. No. 5,538,880 (corn); U.S. Pat. No. 5,914,451 (soybean); U.S. Pat. No. 6,160,208 (corn); U.S. Pat. No. 6,399,861 (corn) and U.S. Pat. No. 6,153,812 (wheat) and Agrobacterium-mediated transformation is described in U.S. Pat. No. 5,159,135 (cotton); U.S. Pat. No. 5,824,877 (soybean); U.S. Pat. No. 5,591,616 (corn); and U.S. Pat. No. 6,384,301 (soybean), all of which are incorporated herein by reference. For Agrobacterium tumefaciens based plant transformation system, additional elements present on transformation constructs will include T-DNA left and right border sequences to facilitate incorporation of the recombinant polynucleotide into the plant genome.

[0046] In general it is useful to introduce recombinant DNA randomly, i.e. at a non-specific location, in the genome of a target plant line. In special cases it may be useful to target recombinant DNA insertion in order to achieve site-specific integration, for example to replace an existing gene in the genome, to use an existing promoter in the plant genome, or to insert a recombinant polynucleotide at a predetermined site known to be active for gene expression. Several site specific recombination systems exist which are known to function implants include cre-lox as disclosed in U.S. Pat. No. 4,959,317 and FLP-FRT as disclosed in U.S. Pat. No. 5,527,695.

[0047] Transformation methods of this invention are preferably practiced in tissue culture on media and in a controlled environment. "Media" refers to the numerous nutrient mixtures that are used to grow cells in vitro, that is, outside of the intact living organism. Recipient cell targets include, but are not limited to, meristem cells, callus, immature embryos and gametic cells such as microspores, pollen, sperm and egg cells. It is contemplated that any cell from which a fertile plant may be regenerated is useful as a recipient cell. Callus may be initiated from tissue sources including, but not limited to, immature embryos, seedling apical meristems, microspores and the like. Cells capable of proliferating as callus are also recipient cells for genetic transformation. Practical transformation methods and materials for making transgenic plants of this invention, for example various media and recipient target cells, transformation of immature embryo cells and subsequent regeneration of fertile transgenic plants are disclosed in U.S. Pat. Nos. 6,194,636 and 6,232,526, which are incorporated herein by reference.

[0048] The seeds of transgenic plants can be harvested from fertile transgenic plants and be used to grow progeny generations of transformed plants of this invention including hybrid plants line for selection of plants having an enhanced trait. In addition to direct transformation of a plant with a recombinant DNA, transgenic plants can be prepared by crossing a first plant having a recombinant DNA with a second plant lacking the DNA. For example, recombinant DNA can be introduced into first plant line that is amenable to transformation to produce a transgenic plant which can be crossed with a second plant line to introgress the recombinant DNA into the second plant line. A transgenic plant with recombinant DNA providing an enhanced trait, e.g. enhanced stress tolerance and/or yield, can be crossed with a transgenic plant line having other recombinant DNA that confers another trait or traits, for example herbicide resistance or pest resistance, to produce progeny plants having recombinant DNA that confers multiple traits. Typically, in breeding to combine traits the transgenic plant donating the additional trait is a male line and the transgenic plant carrying the base trait or traits is the female line. The progeny of this cross will segregate such that some of the plants will carry the DNA for traits present in both parents and some will carry DNA for the trait or traits from only one parent; such plants can be identified by markers associated with parental recombinant DNA, e.g. marker identification by analysis for recombinant DNA or, in the case where a selectable marker is linked to the recombinant, by application of the selecting agent such as a herbicide for use with a herbicide tolerance marker, or by selection for the enhanced trait. Progeny plants carrying DNA for traits from both parents can be crossed back into the female parent line multiple times, for example usually 6 to 8 generations, to produce a progeny plant with substantially the same genotype as one original transgenic parental line but for the recombinant DNA of the other transgenic parental line.

[0049] In the practice of transformation DNA is typically introduced into only a small percentage of target plant cells in any one transformation experiment. Marker genes are used to provide an efficient system for identification of those cells that are stably transformed by receiving and integrating a transgenic DNA construct into their genomes. Preferred marker genes provide selective markers which confer resistance to a selective agent, such as an antibiotic or herbicide. Any of the herbicides to which plants of this invention may be resistant are useful agents for selective markers. Potentially transformed cells are exposed to the selective agent. In the population of surviving cells will be those cells where, generally, the resistance-conferring gene is integrated and expressed at sufficient levels to permit cell survival. Cells may be tested further to confirm stable integration of the exogenous DNA. Commonly used selective marker genes include those conferring resistance to antibiotics such as kanamycin and paromomycin (nptII), hygromycin B (aph IV) and gentamycin (aac3 and aacC4) or resistance to herbicides such as glufosinate (bar or pat) and glyphosate (aroA or EPSPS). Examples of such selectable are illustrated in U.S. Pat. Nos. 5,550,318; 5,633,435; 5,780,708 and 6,118,047. Selectable markers which provide an ability to visually identify transformants can also be employed, for example, a gene expressing a colored or fluorescent protein such as a luciferase or green fluorescent protein (GFP) or a gene expressing a beta-glucuronidase or uidA gene (GUS) for which various chromogenic substrates are known.

[0050] Transgenic plant cells that survive exposure to the selective agent or transgenic plant cells that have been scored positive in a screening assay are typically cultured in regeneration media and allowed to mature into plants. For example developing plantlets regenerated from transformed plant cells can be transferred to plant growth mix, and hardened off, for example, in an environmentally controlled chamber at about 85% relative humidity, 600 ppm CO.sub.2, and 25-250 microeinsteins m.sup.-2 s.sup.-1 of light, prior to transfer to a greenhouse or growth chamber for maturation. Plants are regenerated from about 6 weeks to 10 months after a transformant is identified, depending on the initial tissue. Plants may be pollinated using conventional plant breeding methods known to those of skill in the art and seed produced, for example self-pollination is commonly used with transgenic corn. The non-natural regenerated transgenic plants or progeny plants can be tested for the presence and expression of the recombinant DNA and selected for the presence of enhanced stress tolerance.

Transgenic Plants and Seeds

[0051] Non-natural transgenic plants derived from the plant cells of this invention are grown to generate transgenic plants having an enhanced trait as compared to a control plant and produce transgenic seed and haploid pollen of this invention. Such plants with enhanced traits are identified by selection of transformed plants or progeny seed for the enhanced trait. For efficiency a selection method is designed to evaluate multiple transgenic plants (events) including the recombinant DNA, for example multiple plants from 2 to 20 or more transgenic events. Transgenic plants grown from transgenic seed provided herein demonstrate enhanced stress tolerance that contributes to increased yield.

[0052] Not all transgenic events will be in transgenic plant cells that provide plants and seeds with an enhanced or desired trait depending on factors, such as location and integrity of the recombinant DNA, copy number, unintended insertion of other DNA, etc. As a result transgenic plant cells of this invention are identified by screening transformed progeny plants for enhanced stress tolerance and yield. For efficiency a screening program is designed to evaluate multiple transgenic plants preferably with a single copy of the recombinant DNA from two or more transgenic events.

[0053] Transgenic plants having enhanced water use efficiency are identified by screening plants in an assay where water is withheld for period to induce stress followed by watering to revive the plants. For example, a useful selection process for water defect tolerant transgenic corn plants imposes 3 drought/re-water cycles on plants over a total period of 15 days after an initial stress free growth period of 11 days. Each cycle consists of 5 days, with no water being applied for the first four days and a water quenching on the 5th day of the cycle. The primary phenotypes analyzed by the selection method are the changes in plant growth rate as determined by height and biomass during a vegetative drought treatment.

[0054] Transgenic plants having enhanced cold tolerance are identified by screening plants in a cold germination assay and/or a cold tolerance field trial. In a cold germination assay trays of transgenic and control seeds are placed in a growth chamber at 9.7.degree. C. for 24 days (no light). Seeds having higher germination rates as compared to the control are identified as having enhanced cold tolerance. In a cold tolerance field trial plants with enhanced cold tolerance are identified from field planting at an earlier date than conventional Spring planting for the field location. For example, seeds are planted into the ground around two weeks before local farmers begin to plant corn so that a significant cold stress is exerted onto the crop, named as cold treatment. Seeds also are planted under local optimal planting conditions such that the crop has little or no exposure to cold condition, named as normal treatment. At each location, seeds are planted under both cold and normal conditions preferably with multiple repetitions per treatment.

[0055] Transgenic plants having enhanced yield are identified by screening using progeny of the transgenic plants over multiple locations with plants grown under optimal production management practices and maximum weed and pest control. A useful target for improved yield is a 5% to 10% increase in yield as compared to yield produced by plants grown from seed for a control plant. Selection methods may be applied in multiple and diverse geographic locations, for example up to 16 or more locations, over one or more planting seasons, for example at least two planting seasons, to statistically distinguish yield improvement from natural environmental effects.

[0056] The following examples are included to demonstrate embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples that follow represent techniques discovered by the inventors to function well in the practice of the invention. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention, therefore all matter set forth or shown in the accompanying drawings and examples is to be interpreted as illustrative and not in a limiting sense.

Example 1

[0057] This example illustrates the preparation of recombinant DNA encoding proteins derived from bacterial cold shock proteins. Recombinant DNA is prepared which encodes proteins having single amino acid changes at amino acid positions F15, F17, F27, H29 and F30 of Bs-CspB_L2V (SEQ ID NO:1) using degenerate 2'-OMe primers. DNA generated in this manner encodes proteins with the amino acid sequence of SEQ ID NO:53 through SEQ ID NO:57 and SEQ ID NO:458 through SEQ ID NO:578.

[0058] Recombinant DNA is prepared which encodes proteins with Single amino acid changes at positions S31 and T40 of Bs-CspB_L2V (SEQ ID NO:1). DNA generated in this manner encodes proteins with amino acid sequence of SEQ ID NO:32, SEQ ID NO:33 and SEQ ID NO:428 through SEQ ID NO:457.

[0059] Recombinant DNA was prepared which encodes protein variants of Bs-CspB with two amino acid residues, N and N+2 (where N is any one of amino acids 2-65 of SEQ ID NO:1), replaced with alanine residues. Where the amino acid at position N or N+2 is alanine, a serine was substituted for alanine. DNA generated in this manner encodes proteins with the amino acid sequence of SEQ ID NO:275 through SEQ ID NO:312, Other recombinant DNAs encoding protein variants of Bs-CspB with multiple alanine substitutions were made and encode proteins with the amino acid sequence of SEQ ID NO:270 through SEQ ID NO:274.

[0060] Recombinant DNAs were also prepared by exchanging reciprocal DNA regions encoding the amino acid regions identified as "Reg 1"-"Reg 5" (See FIG. 1) of the bacterial cold shock proteins of SEQ ID NOS: 1-7. DNA generated by region exchange encoded proteins having the amino acid sequence of SEQ ID NO:16 through SEQ ID NO:23 and SEQ ID NO:313 through SEQ ID NO:427. The proteins encoded by the DNAs generated in this manner are designated as chimeric in the sequence listing. The name in the comments field for each protein indicates the donor region and the backbone sequence for the regions in the variant proteins. For example, Chimeric Tm_Csp1_R2_Bs_CspB_bb refers to a chimeric variant bacterial cold shock protein sequence (SEQ ID NO:17) having the R2 region of Thermotoga maritime Csp 1 exchanged into the backbone sequence of Bacillus subtilis CspB.

[0061] Recombinant DNA encoding variant proteins of Bs-CspB having modifications in identified areas of sequence diversity and amino acid sequences that lie within the loop regions of Bs-CspB are generated using a degenerate oligonucleotide tail approach. The regions and specific targeted amino acid substitutions for variant generation are shown in FIG. 2. DNAs produced as the result of this approach encode proteins having amino acid sequences of SEQ ID NO:15, SEQ ID NO:24 through SEQ ID NO:31, SEQ ID NO:58 through SEQ ID NO:269 and SEQ ID NO:579 through SEQ ID NO:704.

[0062] Proteins encoded by the recombinant DNAs were expressed, purified and analyzed to determine their ability to complement the cold sensitivity of a quadruple E. coli mutant, BX04 (DE3) and to determine their ability to destabilize single stranded DNA secondary structure and bind to single stranded DNA. Constructs for expression of the proteins in transgenic plants are prepared using each of the recombinant DNAs operably linked to a promoter operable in plant cells.

Example 2

[0063] This example, illustrates preparation of recombinant DNA constructs for transformation of corn, rice, Arabidopsis, soybean, and cotton.

[0064] Plant Expression Constructs for Corn Transformation.

[0065] A base vector useful for Agrobacterium-mediated transformation of corn, pMON74590, is shown in FIG. 3. The vector provides an expression cassette for expression of DNA encoding proteins prepared in Example 1 under the control of an enhanced 35S/rice actin promoter (SEQ ID NO:706). The transformation vector also contains an EPSPS gene as a selectable marker for resistance to glyphosate herbicide. The glyphosate resistance expression cassette comprises a rice actin I promoter, leader and intron operably linked to a DNA encoding a chloroplast transit peptide from an Arabidopsis thaliana EPSPS gene and DNA encoding an EPSPS from an A. tumefaciens gene (CP4) and a 3' element from an A. A. tumefaciens nopaline synthase gene. DNA having the sequence of SEQ ID NO: 34 through SEQ ID NO:52, encoding proteins prepared in Example 1, is cloned into pMON74590 and the resulting constructs are used for production of transgenic corn plants expressing variant bacterial cold shock proteins of SEQ ID NO:15 through SEQ ID NO:33.

[0066] Plant Expression Constructs for Rice Transformation

[0067] A base vector useful for Agrobacterium-mediated transformation of rice, pMON112481, is shown in FIG. 4. The vector provides for enhanced protein expression under the control of a rice GOS2 gene promoter (SEQ ID NO:705). The transformation vector also contains an EPSPS gene as a selectable marker for resistance to glyphosate herbicide. The glyphosate resistance expression cassette comprises a rice actin 1 promoter, leader and intron operably linked to a DNA encoding a chloroplast transit peptide from an Arabidopsis thaliana EPSPS gene and DNA encoding an EPSPS from an A. tumefaciens gene (CP4) and a 3' element from an A. tumefaciens nopaline synthase gene. DNA having the sequence of SEQ ID NO: 34 through SEQ ID NO:52, encoding proteins prepared in Example 1, is cloned into pMON112481 and the resulting constructs are used for production of transgenic corn plants expressing variant bacterial cold shock proteins of SEQ ID NO:15 through SEQ ID NO:33.

[0068] Plant Expression Constructs for Transformation of Arabidopsis

[0069] A base vector useful for Agrobacterium-mediated transformation of Arabidopsis, pMON100407, is shown in FIG. 5. The vector provides for enhanced protein expression under the control of an enhanced Cauliflower Mosaic Virus 35S promoter (U.S. Pat. No. 5,359,142). The transformation vector also contain an EPSPS gene as a selectable marker for resistance to glyphosate herbicide. The glyphosate resistance expression cassette comprises a rice actin 1 promoter, leader and intron operably linked to a DNA encoding a chloroplast transit peptide from an Arabidopsis thaliana EPSPS gene and DNA encoding an EPSPS from an A. tumefaciens gene (CP4) and a 3' element from an A. tumefaciens nopaline synthase gene. DNA having the sequence of SEQ ID NO: 34 through SEQ ID NO:52, encoding proteins prepared in Example 1, is cloned into pMON100407 and the resulting constructs are used for production of transgenic Arabidopsis plants expressing variant bacterial cold shock proteins of SEQ ID NO:15 through SEQ ID NO:33. Additional constructs are generated using recombinant DNA encoding variant bacterial cold shock proteins of SEQ ID NO:258, SEQ ID NO:268, SEQ ID NO:331, SEQ ID NO:333, SEQ ID NO:334, SEQ ID NO:343, SEQ ID NO:353, SEQ ID NO:355, SEQ ID NO:367, SEQ ID NO:377, SEQ ID NO:380, SEQ ID NO:381, SEQ ID NO:385, SEQ ID NO:391, SEQ ID NO:397, SEQ ID NO:403, SEQ ID NO:449 and SEQ ID NO:622. The transformation of Arabidopsis plants is carried out using a vacuum infiltration method (Bechtold, e.g., Methods Mol. Biol. 82:259-66, 1998). Seeds harvested from the transgenic plants, T1 seeds, are grown in a glufosinate-containing selective medium to select for transformed plants that produce T2 transgenic seed.

[0070] Plant Expression Constructs for Soybean Transformation

[0071] A base vector useful for Agrobacterium-mediated transformation of soybean, pMON82053, is shown in FIG. 6. The vector provides for enhanced protein expression under the control of an enhanced Cauliflower Mosaic Virus 35S promoter (U.S. Pat. No. 5,359,142). The transformation vector also contain an EPSPS gene as a selectable marker for resistance to glyphosate herbicide. The glyphosate resistance expression cassette comprises a rice actin 1 promoter, leader and intron operably linked to a DNA encoding a chloroplast transit peptide from an Arabidopsis thaliana EPSPS gene and DNA encoding an EPSPS from an A. tumefaciens gene (CP4) and a 3' element from an A. tumefaciens nopaline synthase gene. Recombinant DNA for expression of variant bacterial cold shock proteins is cloned into pMON82053 and the resulting constructs are used for transformation of transgenic soybean plants expressing variant bacterial cold shock proteins.

[0072] Plant Expression Constructs for Cotton Transformation

[0073] A base vector useful for Agrobacterium-mediated transformation of cotton, pMON99053, is shown in FIG. 7. The vector provides for enhanced protein expression under the control of an enhanced CaMV 35S promoter. The transformation vectors also contain an nptII gene as a selectable marker for resistance to antibiotics such as kanamycin. Recombinant DNA for expression of variant bacterial cold shock proteins is cloned into pMON99053 and the resulting constructs are used for transformation of transgenic cotton plants expressing variant bacterial cold shock proteins.

Example 3

[0074] This example illustrates the production of multiple transgenic events of transgenic corn plants expressing each of the recombinant DNAs prepared in Example 1 using the vectors prepared in Example 2.

[0075] Transgenic corn plants are made from cells transformed by Agrobacterium mediated transformation using DNA constructs prepared in Example 2. Transgenic plants having recombinant DNA stably inserted in the chromosome and expressing proteins having the amino acid sequences of SEQ ID NO:15 through SEQ ID NO:33, are evaluated for stress tolerance, including yield under water deficit stress. Events of these transgenic plants are identified that have increased tolerance to abiotic stress as compared to control plants. Transgenic seed is collected from the identified plants.

Example 4

[0076] Transgenic rice plants are prepared by Agrobacterium mediated transformation using DNA constructs described in Example 2. Transgenic plants having recombinant DNA stably inserted in the chromosome and expressing a protein prepared in Example 1 are identified and evaluated for stress tolerance, including yield under water deficit stress. Events of these transgenic plants are identified that have increased tolerance to abiotic stress as compared to control plants. Transgenic seed is collected from the identified plants.

Example 5

[0077] Transgenic cotton plants are prepared by Agrobacterium mediated transformation using DNA constructs described in Example 2. Transgenic plants having recombinant DNA stably inserted in the chromosome and expressing a protein prepared in Example 1 are identified and evaluated for stress tolerance, including yield under water deficit stress. Events of these transgenic plants are identified that have increased tolerance to abiotic stress as compared to control plants. Transgenic seed is collected from the identified plants.

Example 6

[0078] Transgenic soybean plants are prepared by Agrobacterium mediated transformation using DNA constructs described in Example 2. Transgenic plants having recombinant DNA stably inserted in the chromosome and expressing a protein prepared in Example 1 are identified and evaluated for stress tolerance, including yield under water deficit stress. Events of these transgenic plants are identified that have increased tolerance to abiotic stress as compared to control plants. Transgenic seed is collected from the identified plants.

Example 7

[0079] Transgenic alfalfa, canola, switchgrass and sugarcane plants comprising DNA constructs stably inserted in the chromosome and expressing a protein prepared in Example 1 are prepared and evaluated for stress tolerance, including yield under water deficit stress. Events of these transgenic plants are identified that have increased tolerance to abiotic stress as compared to control plants. Transgenic seed is collected from the identified plants.

Example 8

[0080] This example illustrates the various stress tolerance properties of heterozygous hybrid corn plants expressing a protein with a cold shock domain. Corn plants were screened for enhanced cold stress tolerance by measuring germination and/or early seedling growth at cold temperature, water deficit stress tolerance in a greenhouse "drought" screen, and water deficit tolerance in a random field screen in which plants from randomly planted seed were subjected to water deficit allowing an investigator to observe and select plants with positive water deficit tolerance.

Reference is made to Table 1 showing the number of transgenic events tested for recombinant DNA expressing the various proteins and whether events were identified as showing water deficit or cold stress tolerance.

TABLE-US-00001 TABLE 1 Water stress Water stress Increased SEQ tolerance in tolerance in yield under ID greenhouse random field water stress Cold stress Expressed protein NO: screen screen (field screen) tolerance B. subtilis cspB L2V 1 Yes Yes No E. coli cspC 4 Yes Yes E. coli cspD 5 Yes No No Agrobacterium 6 No No tumefaciens Csp2 Agrobacterium Yes Yes Yes tumefaciens Csp4 E. coli cspA Yes No No E. coli cspG 7 No No Yes Arabidopsis csp-like 1 Yes No No cotton csp-like 1 Yes No No Yes cotton csp-like 2 Yes No Yes Yes Bs_cspB-L2V-F3OR 53 Yes No Bs_cspB-L2V-F15Y 54 Yes No Bs_cspB-L2V-F15R 55 No No Bs_cspB-L2V-F3OW 56 No No Bs_cspB-L2V- 57 No No F15Y:F3OW

All of the materials and methods disclosed and claimed herein can be made and used without undue experimentation as instructed by the above disclosure. Although the materials and methods of this invention have been described in terms of preferred embodiments and illustrative examples, it will be apparent to those of skill in the art that variations may be applied to the materials and methods described herein without departing from the concept, spirit and scope of the invention. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.

Sequence CWU 1

1

708167PRTArtificial Sequencesynthetic sequence, Bs-CspBL2V 1Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 266PRTThermotoga maritimaTm_Csp1 2Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 366PRTThermoanaerobacter tengcongensisTt_Csp1 3Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 469PRTEscherichia coliEc_CspC 4Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 574PRTEscherichia coliEc_CspD 5Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 667PRTAgrobacterium tumefaciensAb_Csp2 6Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gly Phe Gly Gly Ser Leu Gln Asp Gly Gln Lys Val Ser Tyr 35 40 45 Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu Asn Val 50 55 60 Thr Leu Leu 65 770PRTEscherichia coliEc_CspG 7Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 8204DNAArtificial Sequencesynthetic sequence, Bs-CspBL2V DNA 8atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtag 2049201DNAThermotoga maritimaTm_Csp1 DNA 9atgcgcggca aggtgaagtg gttcgactcc aagaagggct acggcttcat cacgaaggac 60gagggcggcg atgtcttcgt gcactggagc gccatcgaga tggagggctt caagacgctc 120aaggagggtc aagtggtcga gttcgagatt caagagggaa agaaaggtcc gcaggccgcg 180cacgtcaagg tcgtcgaatg a 20110201DNAThermoanaerobacter tengcongensisTt_Csp1 DNA 10atggtgcgcg gcaaggtgaa gtggttcaac gctgagaagg gctacggctt catcgagcgc 60gaggacggca ccgacgtctt cgtgcactac tccgccatcg agggcgaggg tttcaagacg 120ctggaggagg gacaggccgt cgagttcgaa gtcgtccaag ccgcgaaggg tccacaggcg 180agcaaggtcc ggaaactgtg a 20111210DNAEscherichia coliEc_CspC DNA 11atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt ctaaaggttt tggcttcatt 60actccggctg atggcagcaa agatgtgttc gtacacttct ccgctatcca gggtaatggc 120ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa ttcaggacgg ccagaaaggt 180ccggcagctg ttaacgtaac agctatctag 21012225DNAEscherichia coliEc_CspD DNA 12atggaaaagg gtactgttaa gtggttcaac aatgccaaag ggtttggttt catctgccct 60gaaggcggcg gcgaagatat tttcgctcat tattccacca ttcagatgga tggttacaga 120acgctaaaag ctggacaatc cgttcagttt gatgtccacc aggggccaaa aggcaatcac 180gccagtgtta ttgtgcccgt cgaagtagaa gcggcagtcg catag 22513204DNAAgrobacterium tumefaciensAb_Csp2 DNA 13atggcaactg gcactgtaaa attcttcgct caggacaagg gctttggctt cattacccct 60gacaatggcg gtcctgacgt attcgttcac atctcggcag tcggtttcgg cggctctctt 120caggatggtc agaaggtgag ctacgagttg ggacaagacc gcaagaccgg taaatcgaaa 180gccgagaacg tcactctcct ttag 20414213DNAEscherichia coliE. coli cspG DNA 14atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt 60atcactcctg atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat 120gaattccgca cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt 180ggccccgcgg cagcgaacgt tgttacgctc tag 2131567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 2 15Met Val Glu Gly Lys Val Lys Trp Phe Asn Asp Arg Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 1674PRTArtificial Sequencesynthetic sequence, Ec_CspD variant 2 Chimeric Tm_Csp1_R1_Ec_CspD_bb 16Met Glu Lys Gly Thr Val Lys Trp Phe Asp Ser Lys Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 1767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 5 Chimeric Tm_Csp1_R2_Bs_CspB_bb 17Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 1874PRTArtificial Sequencesynthetic sequence, Ec_CspD variant 5 Chimeric Bs_CspB_R3_Ec_CspD_bb 18Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Gly Glu Gly Phe Lys Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 1967PRTArtificial Sequencesynthetic sequence, Ab_Csp2 variant 1 Chimeric Ec_CspC_R4_Ab_Csp2_bb 19Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gly Phe Gly Gly Ser Leu Gln Asp Gly Gln Lys Val Ser Tyr 35 40 45 Glu Ile Gln Asp Gly Gln Lys Thr Gly Lys Ser Lys Ala Glu Asn Val 50 55 60 Thr Leu Leu 65 2066PRTArtificial Sequencesynthetic sequence, Tm_Csp1 variant 3 Chimeric Ec_CspC_R4_Tm_Csp1_bb 20Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Asp Gly Gln Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 2167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 7 Chimeric Ec_CspC_R5_Bs_CspB_bb 21Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Ala Ala Val Asn Val Thr 50 55 60 Ala Ile Ala 65 2266PRTArtificial Sequencesynthetic sequence, Tt_Csp1 variant 1 Chimeric Tm_Csp1_R3_Tt_Csp1_bb 22Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Met Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 2369PRTArtificial Sequencesynthetic sequence, Ab_Csp2 variant 2 Chimeric Tm_Csp1_R3_Ab_Csp2_bb 23Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Glu Met Glu Gly Phe Lys Thr Leu Gln Asp Gly Gln Lys Val 35 40 45 Ser Tyr Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu 50 55 60 Asn Val Thr Leu Leu 65 2467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 8 24Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Ile 50 55 60 Glu Pro Ala 65 2567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 9 25Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Lys Val Lys 50 55 60 Ile Lys Ala 65 2676PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 10 26Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Ile 50 55 60 Lys Leu Ala Ser Trp Gln Ser Tyr Lys Thr Ser Val 65 70 75 2767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 11 27Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Gln Val Val 50 55 60 Asn Gln Arg 65 2868PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 13 28Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Cys 50 55 60 Asn Thr Ser Val 65 2967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 14 29Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Thr Asp Gly Ala Asn Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 3067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 15 30Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Asn Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 3165PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 16 31Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Glu 50 55 60 Arg 65 3267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 17 32Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Cys Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 3367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 18 33Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Gly Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35

40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 34204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 2 34atggtagaag gtaaagtaaa atggttcaac gacagaaaag gttacggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtga 20435225DNAArtificial Sequencesynthetic sequence, Ec_CspD variant 2 35atggaaaagg gtactgttaa gtggttcgac tccaagaaag ggtttggttt catctgccct 60gaaggcggcg gcgaagatat tttcgctcat tattccacca ttcagatgga tggttacaga 120acgctaaaag ctggacaatc cgttcagttt gatgtccacc aggggccaaa aggcaatcac 180gccagtgtta ttgtgcccgt cgaagtagaa gcggcagtcg catag 22536204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 5 36atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcacgaag 60gacgagggcg gcgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtga 20437225DNAArtificial Sequencesynthetic sequence, Ec_CspD variant 5 37atggaaaagg gtactgttaa gtggttcaac aatgccaaag ggtttggttt catctgccct 60gaaggcggcg gcgaagatat tttcgctcat tattccacca ttcaaggcga aggtttcaaa 120acgctaaaag ctggacaatc cgttcagttt gatgtccacc aggggccaaa aggcaatcac 180gccagtgtta ttgtgcccgt cgaagtagaa gcggcagtcg catag 22538204DNAArtificial Sequencesynthetic sequence, Ab_Csp2 variant 1 38atggcaactg gcactgtaaa attcttcgct caggacaagg gctttggctt cattacccct 60gacaatggcg gtcctgacgt attcgttcac atctcggcag tcggtttcgg cggctctctt 120caggatggtc agaaggtgag ctacgagatt caggacggcc agaagaccgg taaatcgaaa 180gccgagaacg tcactctcct ttag 20439201DNAArtificial Sequencesynthetic sequence, Tm_Csp1 variant 3 39atgcgcggca aggtgaagtg gttcgactcc aagaagggct acggcttcat cacgaaggac 60gagggcggcg atgtcttcgt gcactggagc gccatcgaga tggagggctt caagacgctc 120aaggagggtc aagtggtcga gttcgagatt caagacggac agaaaggtcc gcaggccgcg 180cacgtcaagg tcgtcgaatg a 20140204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 7 40atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accagcagct 180gttaacgtta ctgctatcgc gtga 20441201DNAArtificial Sequencesynthetic sequence, Tt_Csp1 variant 1 41atggtgcgcg gcaaggtgaa gtggttcaac gctgagaagg gctacggctt catcgagcgc 60gaggacggca ccgacgtctt cgtgcactac tccgccatcg agatggaggg tttcaagacg 120ctggaggagg gacaggccgt cgagttcgaa gtcgtccaag ccgcgaaggg tccacaggcg 180agcaaggtcc ggaaactgtg a 20142210DNAArtificial Sequencesynthetic sequence, Ab_Csp2 variant 2 42atggcaactg gcactgtaaa attcttcgct caggacaagg gctttggctt cattacccct 60gacaatggcg gtcctgacgt attcgttcac atctcggcag tcgagatgga gggcttcaag 120acgcttcagg atggtcagaa ggtgagctac gagttgggac aagaccgcaa gaccggtaaa 180tcgaaagccg agaacgtcac tctcctttag 21043204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 8 43atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180atccaagtta tagaaccagc gtga 20444204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 9 44atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180ggcaaagtta aaataaaagc gtga 20445270DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 10 45atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180atccaagtta taaaactagc aagctggcaa agttacaaaa ccagcgtgac tcgagcacca 240ccaccaccac cactgagatc cggctgctaa 27046297DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 11 46atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180ggccaagttg taaaccagcg tgactcgagc accaccacca ccaccactga gatccggctg 240ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactag 29747246DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 13 47atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gtccaagttt gtaatactag cgtgactcga gcaccaccac caccaccact gagatccggc 240tgctaa 24648204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 14 48atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcacaacg 60gatggtgcaa acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtga 20449204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 15 49atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaatcc 120ttaaacgaag accaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtga 20450195DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 16 50atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tctgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gtccaagttg agtaa 19551204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 17 51atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc tgcgctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtga 20452204DNAArtificial Sequencesynthetic sequence, Bs-CspB variant 18 52atggtagaag gtaaagtaaa atggttcaac tctgaaaaag gtttcggatt catcgaagta 60gaaggtcaag acgatgtatt cgttcatttc ggggctattc aaggcgaagg cttcaaaact 120ttagaagaag gccaagctgt ttcttttgaa atcgttgaag gaaaccgcgg accacaagct 180gctaacgtta ctaaagaagc gtga 2045367PRTArtificial Sequencesynthetic sequence, Bs_cspB-L2V-F30R 53Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Arg Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 5467PRTArtificial Sequencesynthetic sequence, Bs_cspB-L2V-F15Y 54Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 5567PRTArtificial Sequencesynthetic sequence, Bs_cspB-L2V-F15R 55Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Arg Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 5667PRTArtificial Sequencesynthetic sequence, Bs_cspB-L2V-F30W 56Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Trp Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 5767PRTArtificial Sequencesynthetic sequence, Bs_cspB-L2V-F15YF30W 57Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Trp Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 5867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 58Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Ile Arg Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 5967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 59Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Ser Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 60Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ala Glu Tyr Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 61Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Gly Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 62Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Gly Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 63Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ser Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 64Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 65Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Asp Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 66Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Asp Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 67Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ala Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 68Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asp Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 6967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 69Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asp Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 7067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 70Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Glu Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 7167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 71Met Val Glu Gly Lys Val Lys

Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly His Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 7267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 72Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 7367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 73Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Leu Gln 50 55 60 Lys Lys Arg 65 7467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 74Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 7567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 75Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Val Ala 50 55 60 Lys Gln Ala 65 7667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 76Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Val Glu 50 55 60 Lys Leu Ala 65 7767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 77Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Lys 50 55 60 Ile Thr Ala 65 7867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 78Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Thr 50 55 60 Ala Glu Ala 65 7967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 79Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Lys 50 55 60 Ala Thr Ala 65 8067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 80Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Lys 50 55 60 Thr Thr Ala 65 8167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 81Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Thr 50 55 60 Thr Thr Ala 65 8267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 82Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr His Val Ile 50 55 60 Ile Lys Ala 65 8367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 83Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr His Val Val 50 55 60 Val Pro Ala 65 8467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 84Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Asn Val Ala 50 55 60 Lys Gln Ala 65 8567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 85Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Asn Val Ile 50 55 60 Glu Val Ala 65 8667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 86Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Asn Val Ile 50 55 60 Thr Ala Ala 65 8767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 87Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Asn Val Ile 50 55 60 Val Val Ala 65 8867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 88Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Lys 50 55 60 Ile Ile Ala 65 8967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 89Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val His Val Ile 50 55 60 Ala Ala Ala 65 9064PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 90Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Leu Lys 50 55 60 9167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 91Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Val Ala 50 55 60 Ile Glu Ala 65 9267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 92Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Val Val 50 55 60 Ala Ile Ala 65 9367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 93Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Ile 50 55 60 Ile Ala Ala 65 9467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 94Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Lys 50 55 60 Ile Lys Ala 65 9567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 95Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Lys 50 55 60 Lys Ile Ala 65 9667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 96Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Glu 50 55 60 Ile Leu Ala 65 9767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 97Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Ile 50 55 60 Lys Lys Ala 65 9867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 98Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Lys 50 55 60 Thr Ala Ala 65 9967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 99Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Pro Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 100Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Gln Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 101Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val

Ser 35 40 45 Phe Glu Ile Val Glu Gly Arg Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 102Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Arg Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 103Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ser Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 104Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Thr Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 105Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Val Val Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 106Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Ile Gln Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 107Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 108Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Gly Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 10967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 109Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Asn Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 110Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Ser Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 111Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Thr Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 112Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Arg Glu Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 113Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 114Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 115Met Val Glu Gly Lys Val Lys Trp Phe Asp Thr Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 116Met Val Glu Gly Lys Val Lys Trp Phe Asn Asp Ser Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 117Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Ala Met Asp Gly Pro Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 118Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Ala Met Asp Gly Pro Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 11967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 119Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Ala Ala Gly Lys Asn Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 120Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Ala Asp Gly Thr Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 121Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Ala Asp Gly Thr Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 122Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Leu Asp Gly Pro Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 123Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Leu Asp Gly Pro Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 124Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Met Glu Gly Gln Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 125Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Met Glu Gly Gln Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 126Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Gln Glu Gly Arg Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 127Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Gln Glu Gly Arg Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 128Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Thr Asp Gly Ala Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 12967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 129Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Thr Asp Gly Ala Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 130Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Ala Gly Lys Ser Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 131Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Pro Arg Glu Gly Ala Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe

Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 132Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Glu Asp Gly Ala Gly Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 133Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Glu Asp Gly Ala Gly Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 134Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Gly Asp Gly Glu Ser Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 135Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Gly Asp Gly Glu Ser Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 136Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Gln Asp Gly Pro Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 137Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Arg Glu Gly Gly Gly Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 138Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Arg Glu Gly Gly Gly Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 13967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 139Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Arg Glu Gly Gly Gly Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 140Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Thr Ala Gly Gly Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 141Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Thr Glu Gly Thr Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 142Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Thr Glu Gly Thr Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 143Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Val Ala Gly Thr Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 144Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Gln Val Ala Gly Thr Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 145Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Thr Asp Gly Ala Asn Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 146Met Val Glu Gly Lys Val Lys Trp Phe Thr Ala Asp Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 147Met Val Glu Gly Lys Val Lys Trp Phe Thr Asp Lys Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 148Met Val Glu Gly Lys Val Lys Trp Phe Thr Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 14967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 149Met Val Glu Gly Lys Val Lys Trp Phe Thr Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 150Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Ala Ser Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 151Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Gly Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 152Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Gly Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 153Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Gly Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 154Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Gly Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 155Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Lys Asp Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 156Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Lys Ser Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 157Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Lys Ser Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 158Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Asn Glu Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 15967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 159Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Asn Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 160Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Asn Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 161Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Glu Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50

55 60 Lys Glu Ala 65 16267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 162Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Glu Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 163Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Gly Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 164Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Gly Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 165Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 166Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Ser Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 167Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 168Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 16967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 169Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asp Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 170Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asp Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 171Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Asp Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 172Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Asp Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 173Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ala Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 174Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 175Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 176Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 177Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Ala Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 178Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ala Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 17967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 179Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Lys Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18070PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 180Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Pro Lys Gly Pro Lys Gly Pro Gln Ala Ala 50 55 60 Asn Val Thr Lys Glu Ala 65 70 18167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 181Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Arg Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 182Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Thr Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 183Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 184Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 185Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Asn Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 186Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ile Glu Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 187Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ile Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 188Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ile Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 18967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 189Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Lys Asp Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 190Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Lys Asp Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 191Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Lys Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65

19267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 192Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Lys Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 193Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Lys Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 194Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Met Lys Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Asn Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 195Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Asp Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 196Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 197Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 198Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Lys Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 19967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 199Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 200Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Asn Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 201Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 202Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 203Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Ser Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 204Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Asn Ser Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 205Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Arg Asp Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 206Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Arg Glu Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 207Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Thr Asn Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 208Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Thr Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 20967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 209Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Thr Ser Gly Tyr Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 210Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Val Gly Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 211Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Val Gly Gly Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 212Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Val Asn Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 213Met Val Glu Gly Lys Val Lys Trp Phe Ala Ala Gly Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 214Met Val Glu Gly Lys Val Lys Trp Phe Asn Asp Asn Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 215Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Thr Ser Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 216Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Gly Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 217Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ser Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 218Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Ala Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 21967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 219Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 22067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 220Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ala Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 22167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 221Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ala Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 22267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 222Met Val

Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Cys Gly Pro Gln Ala Ser Asn Val Pro 50 55 60 Ala Thr Ala 65 22367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 223Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala His Val Glu 50 55 60 Glu Thr Ala 65 22467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 224Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Gln Val Ala 50 55 60 Thr Thr Ala 65 22567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 225Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Gln Val Glu 50 55 60 Lys Lys Ala 65 22667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 226Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Gln Val Thr 50 55 60 Lys Glu Ala 65 22767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 227Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly His Val Val 50 55 60 Ile Lys Ala 65 22867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 228Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Lys Val Glu 50 55 60 Glu Lys Ala 65 22967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 229Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Lys Val Glu 50 55 60 Thr Lys Ala 65 23070PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 230Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Gln Ala Ile 50 55 60 Lys Val Glu Glu Thr Ala 65 70 23167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 231Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile His Val Ile 50 55 60 Lys Lys Ala 65 23267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 232Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile His Val Val 50 55 60 Thr Lys Ala 65 23367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 233Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Val Lys 50 55 60 Lys Ile Ala 65 23467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 234Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Ala 50 55 60 Glu Gln Ala 65 23567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 235Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Ile 50 55 60 Glu Pro Ala 65 23667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 236Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Lys 50 55 60 Glu Lys Ala 65 23768PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 237Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Lys 50 55 60 Lys Gln Ala Leu 65 23867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 238Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Lys 50 55 60 Glu Lys Ala 65 23967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 239Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Val 50 55 60 Glu Gln Ala 65 24067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 240Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Lys Val Ile 50 55 60 Glu Glu Ala 65 24168PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 241Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Gln Arg Cys 50 55 60 Lys Arg Ser Val 65 24267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 242Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr His Val Thr 50 55 60 Ile Lys Ala 65 24367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 243Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Lys 50 55 60 Glu Thr Ala 65 24467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 244Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val His Val Ile 50 55 60 Ala Leu Ala 65 24567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 245Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val His Val Ile 50 55 60 Ile Pro Ala 65 24667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 246Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Val Ile 50 55 60 Asn Lys Arg 65 24767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 247Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Arg Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 24867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 248Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Thr Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 24967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 249Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Leu Val Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 250Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ser Leu Val Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 251Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Leu Gln Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 252Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe

Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Val Val Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 253Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 254Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 255Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Lys Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 256Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Lys Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 257Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Asn Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 258Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Asn Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 25967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 259Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Arg Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 260Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Thr Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 261Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Glu Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 262Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Leu Asp Gly Lys Lys Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 263Met Val Glu Gly Lys Val Lys Trp Phe Asn Thr Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 264Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Asn Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 265Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Ala Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 266Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly His Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 267Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Arg Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 268Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Gly Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 26967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 269Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Ser Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27067PRTArtificial Sequencesynthetic sequence, Bs_CspB_G22A_D24A_E3A-K5A 270Met Val Ala Gly Ala Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Ala Gln Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27167PRTArtificial Sequencesynthetic sequence, Bs_CspB_G22A_D24A_G57A-Q59A 271Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Ala Gln Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Ala Pro Ala Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27267PRTArtificial Sequencesynthetic sequence, Bs_CspB_G22A_D24A_T64A-E66A 272Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Ala Gln Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Ala 50 55 60 Lys Ala Ala 65 27367PRTArtificial Sequencesynthetic sequence, Bs_CspB_G22A_D24A_T40A-E42A 273Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Ala Gln Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ala Leu Ala Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27467PRTArtificial Sequencesynthetic sequence, Bs_CspB_G22A_D24A_Q59A-A61S 274Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Ala Gln Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Ala Ala Ser Asn Val Thr 50 55 60 Lys Glu Ala 65 27567PRTArtificial Sequencesynthetic sequence, Bs_CspB_E3A-K5A 275Met Val Ala Gly Ala Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27667PRTArtificial Sequencesynthetic sequence, Bs_CspB_K5A-K7A 276Met Val Glu Gly Ala Val Ala Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27767PRTArtificial Sequencesynthetic sequence, Bs_CspB_W8A-N10A 277Met Val Glu Gly Lys Val Lys Ala Phe Ala Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27867PRTArtificial Sequencesynthetic sequence, Bs_CspB_F9A-S11A 278Met Val Glu Gly Lys Val Lys Trp Ala Asn Ala Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 27967PRTArtificial Sequencesynthetic sequence, Bs_CspB_N10A-E12A 279Met Val Glu Gly Lys Val Lys Trp Phe Ala Ser Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28067PRTArtificial Sequencesynthetic sequence, Bs_CspB_V20A-G22A 280Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Ala Glu Ala Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28167PRTArtificial Sequencesynthetic sequence, Bs_CspB_E21A-Q23A 281Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Ala Gly Ala Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28267PRTArtificial Sequencesynthetic sequence, Bs_CspB_G22A-D24A 282Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1

5 10 15 Phe Ile Glu Val Glu Ala Gln Ala Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28367PRTArtificial Sequencesynthetic sequence, Bs_CspB_D24A-V26A 283Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Ala Asp Ala Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28467PRTArtificial Sequencesynthetic sequence, Bs_CspB_H29A-S31A 284Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Ala Phe Ala Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28567PRTArtificial Sequencesynthetic sequence, Bs_CspB_F30A-A32S 285Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ala Ser Ser 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28667PRTArtificial Sequencesynthetic sequence, Bs_CspB_Q34A-E36A 286Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Ala Gly Ala Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28767PRTArtificial Sequencesynthetic sequence, Bs_CspB_G35A-G37A 287Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Ala Glu Ala Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28867PRTArtificial Sequencesynthetic sequence, Bs_CspB_E36A-F38A 288Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Ala Gly Ala Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 28967PRTArtificial Sequencesynthetic sequence, Bs_CspB_G37A-K39A 289Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Ala Phe Ala Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29067PRTArtificial Sequencesynthetic sequence, Bs_CspB_F38A-T40A 290Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Ala Lys Ala Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29167PRTArtificial Sequencesynthetic sequence, Bs_CspB_T40A-E42A 291Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ala Leu Ala Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29267PRTArtificial Sequencesynthetic sequence, Bs_CspB_L41A-E43A 292Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Ala Glu Ala Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29367PRTArtificial Sequencesynthetic sequence, Bs_CspB_E42A-G44A 293Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Ala Glu Ala Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29467PRTArtificial Sequencesynthetic sequence, Bs_CspB_G44A-A46S 294Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Ala Gln Ser Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29567PRTArtificial Sequencesynthetic sequence, Bs_CspB_Q45A-V47A 295Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Ala Ala Ala Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29667PRTArtificial Sequencesynthetic sequence, Bs_CspB_S48A-E50A 296Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ala 35 40 45 Phe Ala Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29767PRTArtificial Sequencesynthetic sequence, Bs_CspB_E50A-V52A 297Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Ile Ala Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29867PRTArtificial Sequencesynthetic sequence, Bs_CspB_I51A-E53A 298Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ala Val Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 29967PRTArtificial Sequencesynthetic sequence, Bs_CspB_V52A-G54A 299Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Ala Glu Ala Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30067PRTArtificial Sequencesynthetic sequence, Bs_CspB_E53A-N55A 300Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Ala Gly Ala Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30167PRTArtificial Sequencesynthetic sequence, Bs_CspB_G54A-R56A 301Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Ala Asn Ala Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30267PRTArtificial Sequencesynthetic sequence, Bs_CspB_N55A-G57A 302Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ala Arg Ala Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30367PRTArtificial Sequencesynthetic sequence, Bs_CspB_R56A-P58A 303Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Ala Gly Ala Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30467PRTArtificial Sequencesynthetic sequence, Bs_CspB_G57A-Q59A 304Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Ala Pro Ala Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30567PRTArtificial Sequencesynthetic sequence, Bs_CspB_P58A-A60S 305Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Ala Gln Ser Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 30667PRTArtificial Sequencesynthetic sequence, Bs_CspB_Q59A-A61S 306Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Ala Ala Ser Asn Val Thr 50 55 60 Lys Glu Ala 65 30767PRTArtificial Sequencesynthetic sequence, Bs_CspB_N62A-T64A 307Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Ala Val Ala 50 55 60 Lys Glu Ala 65 30867PRTArtificial Sequencesynthetic sequence, Bs_CspB_V63A-K65A 308Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Ala Thr 50 55 60 Ala Glu Ala 65 30967PRTArtificial Sequencesynthetic sequence, Bs_CspB_T64A-E66A 309Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Ala 50 55 60 Lys Ala Ala 65 31067PRTArtificial Sequencesynthetic sequence, Bs_CspB_K65A-A67S 310Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Ala Glu Ser 65 31167PRTArtificial Sequencesynthetic sequence, Bs_CspB_A46S-S48A 311Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ser Val Ala 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 31267PRTArtificial Sequencesynthetic sequence, Bs_CspB_A32S-Q34A 312Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly

Gln Asp Asp Val Phe Val His Phe Ser Ser 20 25 30 Ile Ala Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 31369PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R1_Ec_ CspC_bb 313Met Ala Lys Ile Lys Gly Gln Val Lys Phe Phe Ala Gln Asp Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 31469PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R1_Ec_ CspC_bb 314Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asp Ser Lys Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 31569PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R1_Ec_ CspC_bb 315Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Ala Glu Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 31668PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R2_Ec_ CspC_bb 316Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Phe 20 25 30 Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln Asn 35 40 45 Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val Asn 50 55 60 Val Thr Ala Ile 65 31768PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R2_Ec_ CspC_bb 317Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Phe 20 25 30 Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln Asn 35 40 45 Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val Asn 50 55 60 Val Thr Ala Ile 65 31869PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R3_Ec_ CspC_bb 318Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 31969PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R3_Ec_ CspC_bb 319Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Met Asp Gly Tyr Arg Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 32069PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R2_Ec_ CspC_bb 320Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 32169PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R1_Ec_ CspC_bb 321Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Asn Ala Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 32269PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Ab_ Csp2_bb 322Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gln Gly Asn Gly Phe Lys Thr Leu Gln Asp Gly Gln Lys Val 35 40 45 Ser Tyr Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu 50 55 60 Asn Val Thr Leu Leu 65 32367PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R1_Ab_ Csp2_bb 323Met Ala Thr Gly Thr Val Lys Trp Phe Asp Ser Lys Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gly Phe Gly Gly Ser Leu Gln Asp Gly Gln Lys Val Ser Tyr 35 40 45 Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu Asn Val 50 55 60 Thr Leu Leu 65 32467PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R1_Ab_ Csp2_bb 324Met Ala Thr Gly Thr Val Lys Trp Phe Asn Glu Ser Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gly Phe Gly Gly Ser Leu Gln Asp Gly Gln Lys Val Ser Tyr 35 40 45 Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu Asn Val 50 55 60 Thr Leu Leu 65 32574PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R1_Ec_ CspD_bb 325Met Glu Lys Gly Thr Val Lys Trp Phe Asn Ala Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 32674PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R1_Ec_ CspD_bb 326Met Glu Lys Gly Thr Val Lys Trp Phe Asn Ala Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 32774PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R1_Ec_ CspD_bb 327Met Glu Lys Gly Thr Val Lys Trp Phe Asn Glu Ser Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 32874PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R3_Ec_ CspD_bb 328Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Glu Met Glu Gly Phe Lys Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 32973PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R2_Ec_ CspD_bb 329Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Ile Phe Ala His Tyr Ser Thr 20 25 30 Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val Gln 35 40 45 Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile Val 50 55 60 Pro Val Glu Val Glu Ala Ala Val Ala 65 70 33074PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R2_Ec_ CspD_bb 330Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 33174PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R2_Ec_ CspD_bb 331Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 33274PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R2_Ec_ CspD_bb 332Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 33374PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R1_Ec_ CspD_bb 333Met Glu Lys Gly Thr Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 33466PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R1_Tm_ Csp1_bb 334Met Arg Gly Lys Val Lys Phe Phe Ala Gln Asp Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 33566PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R2_Tm_ Csp1_bb 335Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 33666PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R2_Tm_ Csp1_bb 336Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 33766PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R3_Tm_ Csp1_bb 337Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Gln Gly Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 33867PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R2_Tm_ Csp1_bb 338Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His Trp Ser Ala 20 25 30 Ile Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu 35 40 45 Phe Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys 50 55 60 Val Val Glu 65 33967PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R2_Tm_ Csp1_bb 339Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val His Trp Ser Ala 20 25 30 Ile Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu 35 40 45 Phe Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys 50 55 60 Val Val Glu 65 34067PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R2_Tm_ Csp1_bb 340Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Trp Ser Ala

20 25 30 Ile Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu 35 40 45 Phe Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys 50 55 60 Val Val Glu 65 34166PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R1_Tm_ Csp1_bb 341Met Arg Gly Lys Val Lys Trp Phe Asn Ala Asp Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 34266PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R1_Tm_ Csp1_bb 342Met Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 34366PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R1_Tm_ Csp1_bb 343Met Arg Gly Lys Val Lys Trp Phe Asn Glu Ser Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 34466PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R1_Tm_ Csp1_bb 344Met Arg Gly Lys Val Lys Trp Phe Asn Asn Ala Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 34566PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R1_Tm_ Csp1_bb 345Met Arg Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 34670PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R1_Ec_ CspG_bb 346Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asp Ser Lys Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 34770PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R3_Ec_ CspG_bb 347Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Glu Met Glu Gly Phe Lys Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 34870PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Ec_ CspG_bb 348Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 34970PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R3_Ec_ CspG_bb 349Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Met Asp Gly Tyr Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 35070PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R1_Ec_ CspG_bb 350Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Glu Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 35170PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R1_Ec_ CspG_bb 351Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Glu Ser Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 35270PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R1_Ec_ CspG_bb 352Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Asn Ala Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 35368PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R2_Bs_ CspB_bb 353Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Val Phe Val His Phe Ser 20 25 30 Ala Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val 35 40 45 Ser Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val 50 55 60 Thr Lys Glu Ala 65 35467PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R2_Bs_ CspB_bb 354Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 35567PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R3_Bs_ CspB_bb 355Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 35665PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R3_Bs_ CspB_bb 356Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gly Phe Gly Gly Ser Leu Glu Glu Gly Gln Ala Val Ser Phe Glu 35 40 45 Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr Lys Glu 50 55 60 Ala 65 35767PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Bs_ CspB_bb 357Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Asn Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 35867PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R3_Bs_ CspB_bb 358Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Met Asp Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 35968PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R2_Bs_ CspB_bb 359Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His Phe Ser 20 25 30 Ala Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val 35 40 45 Ser Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val 50 55 60 Thr Lys Glu Ala 65 36068PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R2_Bs_ CspB_bb 360Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Phe Ser 20 25 30 Ala Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val 35 40 45 Ser Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val 50 55 60 Thr Lys Glu Ala 65 36169PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R3_Ec_ CspC_bb 361Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Glu Met Glu Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 36269PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R3_Ec_ CspC_bb 362Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Glu Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 36369PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R4_Ec_ CspC_bb 363Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Asp Val His Gln Gly Pro Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 36469PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R5_Ec_ CspC_bb 364Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Gln Ala Ala 50 55 60 Asn Val Thr Lys Glu 65 36569PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R4_Ec_ CspC_bb 365Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Gln Glu Gly Lys Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 36669PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R4_Ec_ CspC_bb 366Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 36769PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R4_Ec_ CspC_bb 367Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Leu Gly Gln Asp Arg Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 36869PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R4_Ec_ CspC_bb 368Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Glu Val Val Gln Ala Ala Lys Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65

36969PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R4_Ec_ CspC_bb 369Met Ala Lys Ile Lys Gly Gln Val Lys Trp Phe Asn Glu Ser Lys Gly 1 5 10 15 Phe Gly Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His 20 25 30 Phe Ser Ala Ile Gln Gly Asn Gly Phe Lys Thr Leu Ala Glu Gly Gln 35 40 45 Asn Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Ala Ala Val 50 55 60 Asn Val Thr Ala Ile 65 37069PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R3_Ab_ Csp2_bb 370Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Glu Gly Glu Gly Phe Lys Thr Leu Gln Asp Gly Gln Lys Val 35 40 45 Ser Tyr Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu 50 55 60 Asn Val Thr Leu Leu 65 37167PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R4_Ab_ Csp2_bb 371Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gly Phe Gly Gly Ser Leu Gln Asp Gly Gln Lys Val Ser Tyr 35 40 45 Glu Ile Gln Glu Gly Lys Lys Thr Gly Lys Ser Lys Ala Glu Asn Val 50 55 60 Thr Leu Leu 65 37269PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R3_Ab_ Csp2_bb 372Met Ala Thr Gly Thr Val Lys Phe Phe Ala Gln Asp Lys Gly Phe Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Ile Ser 20 25 30 Ala Val Gln Met Asp Gly Tyr Arg Thr Leu Gln Asp Gly Gln Lys Val 35 40 45 Ser Tyr Glu Leu Gly Gln Asp Arg Lys Thr Gly Lys Ser Lys Ala Glu 50 55 60 Asn Val Thr Leu Leu 65 37374PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Ec_ CspD_bb 373Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Gly Asn Gly Phe Lys Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 37474PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R5_Ec_ CspD_bb 374Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn Ala Ala Val Asn Val 50 55 60 Thr Ala Ile Glu Val Glu Ala Ala Val Ala 65 70 37574PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R5_Ec_ CspC_bb 375Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn Gln Ala Ala Asn Val 50 55 60 Thr Lys Glu Glu Val Glu Ala Ala Val Ala 65 70 37674PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R5_Ec_ CspD_bb 376Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Asp Val His Gln Gly Pro Lys Gly Asn Gln Ala Ser Lys Val 50 55 60 Arg Lys Leu Glu Val Glu Ala Ala Val Ala 65 70 37774PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R4_Ec_ CspD_bb 377Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Glu Ile Gln Glu Gly Lys Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 37874PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R4_Ec_ CspD_bb 378Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Glu Ile Val Glu Gly Asn Arg Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 37974PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R4_Ec_ CspD_bb 379Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Glu Leu Gly Gln Asp Arg Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 38074PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R4_Ec_ CspD_bb 380Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Glu Val Val Gln Ala Ala Lys Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 38174PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R4_Ec_ CspD_bb 381Met Glu Lys Gly Thr Val Lys Trp Phe Asn Asn Ala Lys Gly Phe Gly 1 5 10 15 Phe Ile Cys Pro Glu Gly Gly Gly Glu Asp Ile Phe Ala His Tyr Ser 20 25 30 Thr Ile Gln Met Asp Gly Tyr Arg Thr Leu Lys Ala Gly Gln Ser Val 35 40 45 Gln Phe Ser Ile Glu Gln Gly Gln Arg Gly Asn His Ala Ser Val Ile 50 55 60 Val Pro Val Glu Val Glu Ala Ala Val Ala 65 70 38266PRTArtificial Sequencesynthetic sequence, Chimeric Tt_Csp1_R3_Tm_ Csp1_bb 382Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Gly Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 38366PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R4_Tm_ Csp1_bb 383Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Leu Gly Gln Asp Arg Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 38466PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R4_Tm_ Csp1_bb 384Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Glu Met Glu Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Ser Ile Glu Gln Gly Gln Arg Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 38564PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R3_Tm_ Csp1_bb 385Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Gly Phe Gly Gly Ser Leu Lys Glu Gly Gln Val Val Glu Phe Glu Ile 35 40 45 Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val Val Glu 50 55 60 38666PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Tm_ Csp1_bb 386Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Gln Gly Asn Gly Phe Lys Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 38766PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R3_Tm_ Csp1_bb 387Met Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly Phe 1 5 10 15 Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Trp Ser Ala Ile 20 25 30 Gln Met Asp Gly Tyr Arg Thr Leu Lys Glu Gly Gln Val Val Glu Phe 35 40 45 Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala His Val Lys Val 50 55 60 Val Glu 65 38870PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R4_Ec_ CspG_bb 388Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Ala Ala 50 55 60 Ala Asn Val Val Thr Leu 65 70 38970PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R5_Ec_ CspG_bb 389Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Gln Ala 50 55 60 Ala His Val Lys Val Val 65 70 39070PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R5_Tm_ Csp1_bb 390Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Gln Ala 50 55 60 Ala His Val Lys Val Val 65 70 39170PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R5_Ec_ CspG_bb 391Met Ser Asn Lys Met Thr Gly Leu Val Lys Trp Phe Asn Ala Asp Lys 1 5 10 15 Gly Phe Gly Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val 20 25 30 His Phe Thr Ala Ile Gln Ser Asn Glu Phe Arg Thr Leu Asn Glu Asn 35 40 45 Gln Lys Val Glu Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Gln Ala 50 55 60 Ala Asn Val Thr Lys Glu 65 70 39266PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R4_Tt_ Csp1_bb 392Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 39366PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R4_Tt_ Csp1_bb 393Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 39466PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R4_Tt_ Csp1_bb 394Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Leu Gly Gln Asp Arg Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 39566PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R5_Tt_ Csp1_bb 395Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Ala Ala Val Asn Val Thr 50 55 60 Ala Ile 65 39666PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R4_Tt_ Csp1_bb 396Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 39764PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R3_Tt_ Csp1_bb 397Met

Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gly Phe Gly Gly Ser Leu Glu Glu Gly Gln Ala Val Glu Phe Glu 35 40 45 Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg Lys Leu 50 55 60 39866PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R3_Tt_ Csp1_bb 398Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 39966PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Tt_ Csp1_bb 399Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Gly Asn Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 40066PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R3_Tt_ Csp1_bb 400Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Ser Asn Glu Phe Arg Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 40166PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R2_Tt_ Csp1_bb 401Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Thr Lys Asp Glu Gly Gly Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 40267PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R2_Tt_ Csp1_bb 402Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Thr Pro Ala Asp Gly Ser Lys Asp Val Phe Val His Tyr Ser 20 25 30 Ala Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val 35 40 45 Glu Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val 50 55 60 Arg Lys Leu 65 40367PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R2_Tt_ Csp1_bb 403Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Thr Pro Asp Asp Gly Ser Lys Asp Val Phe Val His Tyr Ser 20 25 30 Ala Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val 35 40 45 Glu Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val 50 55 60 Arg Lys Leu 65 40466PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R1_Tt_ Csp1_bb 404Met Val Arg Gly Lys Val Lys Trp Phe Asn Glu Ser Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 40567PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R3_Bs_ CspB_bb 405Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Glu Met Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 40667PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R4_Bs_ CspB_bb 406Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Val His Gln Gly Pro Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 40767PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R4_Bs_ CspB_bb 407Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 40867PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R4_Bs_ CspB_bb 408Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Gln Glu Gly Lys Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 40967PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R5_Bs_ CspB_bb 409Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Lys Ala Glu Asn Val Thr 50 55 60 Leu Leu Ala 65 41067PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R4_Bs_ CspB_bb 410Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ser Ile Glu Gln Gly Gln Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 41166PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R1_Tt_ Csp1_bb 411Met Val Arg Gly Lys Val Lys Phe Phe Ala Gln Asp Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41266PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R1_Tt_ Csp1_bb 412Met Val Arg Gly Lys Val Lys Trp Phe Asp Ser Lys Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41366PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspG_R1_Tt_ Csp1_bb 413Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Asp Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41466PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R4_Tt_ Csp1_bb 414Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Asp Val His Gln Gly Pro Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41566PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R4_Tt_ Csp1_bb 415Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Ile Gln Asp Gly Gln Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41666PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R4_Tt_ Csp1_bb 416Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41766PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R4_Tt_ Csp1_bb 417Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Leu Gly Gln Asp Arg Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 41866PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R5_Tt_ Csp1_bb 418Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Lys Ala Glu Asn Val Thr 50 55 60 Leu Leu 65 41966PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R5_Tt_ Csp1_bb 419Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu 65 42066PRTArtificial Sequencesynthetic sequence, Chimeric Tm_Csp1_R3_Tt_ Csp1_bb 420Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Met Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42166PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R3_Tt_ Csp1_bb 421Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42266PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R3_Tt_ Csp1_bb 422Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Gly Asn Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42366PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspD_R3_Tt_ Csp1_bb 423Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Met Asp Gly Tyr Arg Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42466PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R2_Tt_ Csp1_bb 424Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42567PRTArtificial Sequencesynthetic sequence, Chimeric Ab_Csp2_R2_Tt_ Csp1_bb 425Met Val Arg Gly Lys Val Lys Trp Phe Asn Ala Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Thr Pro Asp Asn Gly Gly Pro Asp Val Phe Val His Tyr Ser 20 25 30 Ala Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val 35 40 45 Glu Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val 50 55 60 Arg Lys Leu 65 42666PRTArtificial Sequencesynthetic sequence, Chimeric Ec_CspC_R1_Tt_ Csp1_bb 426Met Val Arg Gly Lys Val Lys Trp Phe Asn Glu Ser Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20

25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42766PRTArtificial Sequencesynthetic sequence, Chimeric Bs_CspB_R1_Tt_ Csp1_bb 427Met Val Arg Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Arg Glu Asp Gly Thr Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Glu Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Glu 35 40 45 Phe Glu Val Val Gln Ala Ala Lys Gly Pro Gln Ala Ser Lys Val Arg 50 55 60 Lys Leu 65 42867PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31M 428Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Met Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 42967PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31A 429Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ala Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43067PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40L 430Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Leu Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43167PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40R 431Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Arg Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43267PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40W 432Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Trp Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43367PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31Q 433Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Gln Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43467PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40F 434Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Phe Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43567PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40H 435Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys His Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43667PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40K 436Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Lys Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43767PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31T 437Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Thr Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43867PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31R 438Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Arg Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 43967PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31N 439Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Asn Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44067PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40M 440Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Met Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44167PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40E 441Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Glu Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44267PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40Y 442Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Tyr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44367PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31S 443Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44467PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31Y 444Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Tyr Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44567PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40D 445Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Asp Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44667PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40I 446Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ile Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44767PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31W 447Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Trp Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44867PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40V 448Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Val Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 44967PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40C 449Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Cys Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45067PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31L 450Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Leu Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45167PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31I 451Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ile Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45267PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40G 452Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Gly Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45367PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31E 453Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Glu Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45467PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40Q 454Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Gln Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45567PRTArtificial Sequencesynthetic sequence, Bs-CspB_S31D 455Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Asp Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45667PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40S 456Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50

55 60 Lys Glu Ala 65 45767PRTArtificial Sequencesynthetic sequence, Bs-CspB_T40N 457Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Asn Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 458Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Arg Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 45967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 459Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Thr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 460Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Ala Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 461Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Ile Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 462Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Gln Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 463Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Val Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 464Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Asn Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 465Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Trp Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 466Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Arg Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 467Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Ser Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 468Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Leu Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 46967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 469Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Ser Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 470Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Gly Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 471Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Ala Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 472Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Gln Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 473Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 474Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Thr Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 475Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Lys Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 476Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 His Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 477Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Arg Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 478Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Pro Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 47967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 479Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Gly Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 480Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Val Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 481Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Tyr Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 482Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Trp Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 483Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Cys Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 484Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Ile Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 485Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Asp Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 486Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Pro Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65

48767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 487Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Tyr Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 488Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His His Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 48967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 489Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Asn Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 490Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Met Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 491Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Met Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 492Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Ser Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 493Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Val Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 494Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ile Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 495Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Trp Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 496Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Phe Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 497Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Asp Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 498Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Ala Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 49967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 499Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Thr Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 500Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Glu Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 501Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Leu Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 502Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Tyr Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 503Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Leu Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 504Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ala Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 505Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Cys Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 506Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ser Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 507Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Arg Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 508Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Glu Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 50967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 509Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Trp Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 510Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Gly Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 511Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Lys Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 512Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Lys Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 513Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Arg Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 514Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Gln Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 515Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Thr Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 516Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Arg Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 517Met Val

Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Asn Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 518Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Asp Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 51967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 519Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Cys Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 520Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Gln Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 521Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Ile Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 522Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Leu Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 523Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Leu Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 524Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Lys Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 525Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Thr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 526Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Trp Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 527Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Tyr Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 528Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Thr Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 52967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 529Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Trp Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 530Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Tyr Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 531Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Arg Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 532Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Asn Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 533Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Arg Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 534Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Asn Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 535Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Asp Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 536Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His His Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 537Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ile Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 538Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Tyr Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 53967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 539Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Val Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 540Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Ala Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 541Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ser Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 542Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Thr Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 543Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Glu Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 544Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Met Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 545Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Pro Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 546Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Ser Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 547Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5

10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Ala Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 548Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Arg Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 54967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 549Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Gln Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 550Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Gly Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 551Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Glu Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 552Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Gly Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 553Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Lys Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 554Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Met Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 555Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Ser Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 556Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Val Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 557Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Ala Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 558Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Val Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 55967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 559Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Ala Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 560Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Gly Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 561Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Glu Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 562Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Gly Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 563Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Thr Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 564Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Lys Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 565Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Met Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 566Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Arg Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 567Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Val Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 568Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Leu Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 56967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 569Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Trp Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 570Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Ile Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 571Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Cys Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 572Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Tyr Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 573Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Val Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 574Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val Phe Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 575Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Cys Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 576Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Gln Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 577Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln

Asp Asp Val Phe Val His Trp Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 578Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Tyr Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 57967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 579Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Ile Gln Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 580Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 581Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ser Lys Asp His Lys Leu Leu Thr Leu Leu 50 55 60 Lys Lys Arg 65 58267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 582Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Lys Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 583Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Gly Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 584Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Gly Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 585Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Arg Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 586Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Pro Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 587Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Pro Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 588Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Pro Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 58967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 589Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Glu Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 590Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Ser Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 591Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Pro Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 592Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Thr Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 593Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Gly Lys Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 594Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Thr Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 595Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Gly Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 596Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Lys Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 59767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 597Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Glu 50 55 60 Ile Glu Ala 65 59867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 598Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Asn Val Ile 50 55 60 Val Lys Ala 65 59967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 599Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Gln Val Ile 50 55 60 Glu Leu Ala 65 60067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 600Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Glu 50 55 60 Ile Lys Ala 65 60167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 601Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Lys Val Ala 50 55 60 Lys Thr Ala 65 60267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 602Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Ala 50 55 60 Ala Glu Ala 65 60366PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 603Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Asn Val Thr Lys 50 55 60 Glu Ala 65 60467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 604Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Val Ile 50 55 60 Lys Leu Ala 65 60567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 605Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Ile 50 55 60 Lys Val Ala 65 60667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 606Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Lys Val Lys 50 55 60 Lys Glu Ala 65 60767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 607Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25

30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Lys Val Lys 50 55 60 Ile Lys Ala 65 60867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 608Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile His Val Glu 50 55 60 Thr Gln Ala 65 60967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 609Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Val Glu 50 55 60 Ala Ile Ala 65 61067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 610Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Ile 50 55 60 Ile Glu Ala 65 61167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 611Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Gln Val Ile 50 55 60 Lys Leu Ala 65 61267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 612Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile His Val Lys 50 55 60 Ala Pro Ala 65 61367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 613Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Val 50 55 60 Thr Lys Ala 65 61467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 614Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Lys Leu Gln 50 55 60 Lys Lys Arg 65 61567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 615Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Asn Val Glu 50 55 60 Lys Lys Ala 65 61667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 616Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Ala 50 55 60 Glu Val Ala 65 61767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 617Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Val Thr 50 55 60 Ile Thr Ala 65 61867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 618Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Lys Val Ile 50 55 60 Val Ile Ala 65 61967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 619Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Glu 50 55 60 Ile Pro Ala 65 62067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 620Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Leu Lys 50 55 60 Lys Gln Arg 65 62167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 621Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Gln Val Ile 50 55 60 Glu Thr Ala 65 62267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 622Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Gln Val Ala 50 55 60 Ala Pro Ala 65 62367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 623Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Leu Gln 50 55 60 Lys Lys Arg 65 62467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 624Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Gln Val Ile 50 55 60 Lys Gln Ala 65 62567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 625Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Val Glu 50 55 60 Lys Gln Ala 65 62667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 626Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Glu 50 55 60 Val Ile Ala 65 62767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 627Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser His Val Glu 50 55 60 Glu Lys Ala 65 62867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 628Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Val Lys 50 55 60 Lys Lys Ala 65 62967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 629Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Gln Val Ile 50 55 60 Glu Ala Ala 65 63065PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 630Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Glu 50 55 60 Arg 65 63167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 631Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ser Asn Val Lys 50 55 60 Glu Ala Ala 65 63267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 632Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Asn Val Ala 50 55 60 Thr Glu Ala 65 63367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 633Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Val Ile 50 55 60 Lys Thr Ala 65 63467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 634Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Asn Val Ile 50 55 60 Thr Ile Ala 65 63567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 635Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Ala 50 55 60 Ile Gln Ala 65 63667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 636Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Thr Glu Cys Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 63767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 637Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Thr Glu Asn

Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 63867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 638Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 63967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 639Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Glu Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 640Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 641Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ala Glu Asn Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 642Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Gly Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 643Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 644Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Asn Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 645Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Gly Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 646Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Glu Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 647Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ser Glu Ser Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 648Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Thr Glu Asp Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 64967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 649Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Ser Leu Ala Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 650Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Lys Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 651Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Leu Gln Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 652Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Leu Val Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 653Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Leu Leu Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 654Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Ile Gln Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 655Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Val Gln Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 656Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Val Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 657Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Leu Leu Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 658Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Glu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 65967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 659Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Gln Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 660Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Leu Leu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 661Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Val Leu Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 662Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Ile Leu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 663Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Val Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 664Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Lys Leu Val Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 665Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Leu Gln Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 666Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Leu Leu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 667Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45

Phe Lys Leu Val Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 668Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Leu Val Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 66967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 669Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Leu Gln Glu Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 670Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Leu Val Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 671Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Leu Gln Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 672Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Leu Gln Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 673Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Ile Leu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 674Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Leu Gln Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 675Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Gln Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 676Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Val Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 677Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Leu Gln Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 678Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Val Gln Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 67967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 679Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Leu Leu Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 680Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Leu Leu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 681Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Val Leu Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 682Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Ala Val Leu Gln Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 683Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Lys Leu Val Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 684Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Leu Glu Pro Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 685Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Val Gln Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 686Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Leu Leu Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 687Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asp Ile Leu Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 688Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Thr Val Gln Ala Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 68967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 689Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Asn Leu Gln Ser Gly Asn Arg Gly Pro Gln Ala Ala Asn Val Thr 50 55 60 Lys Glu Ala 65 69067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 690Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Val Ala 50 55 60 Thr Gln Ala 65 69167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 691Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Lys Val Glu 50 55 60 Glu Val Ala 65 69268PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 692Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Gln Val Cys 50 55 60 Asn Thr Ser Val 65 69367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 693Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Val Thr 50 55 60 Glu Leu Ala 65 69467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 694Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Gly Lys Val Glu 50 55 60 Glu Lys Ala 65 69567PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 695Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Gln Val Lys 50 55 60 Thr Glu Ala 65 69667PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 696Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Ile 50 55 60 Lys Val Ala 65 69767PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 697Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Ala

50 55 60 Ile Gln Ala 65 69867PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 698Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Val Ala 50 55 60 Ala Thr Ala 65 69967PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 699Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Thr Gln Val Glu 50 55 60 Ile Pro Ala 65 70067PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 700Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ile Lys Leu Lys 50 55 60 Lys Gln Arg 65 70167PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 701Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Asn Val Glu 50 55 60 Thr Lys Ala 65 70267PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 702Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala Lys Leu Gln 50 55 60 Lys Lys Arg 65 70367PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 703Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Ala His Val Glu 50 55 60 Ile Lys Ala 65 70467PRTArtificial Sequencesynthetic sequence, Bs-CspB variant 704Met Val Glu Gly Lys Val Lys Trp Phe Asn Ser Glu Lys Gly Phe Gly 1 5 10 15 Phe Ile Glu Val Glu Gly Gln Asp Asp Val Phe Val His Phe Ser Ala 20 25 30 Ile Gln Gly Glu Gly Phe Lys Thr Leu Glu Glu Gly Gln Ala Val Ser 35 40 45 Phe Glu Ile Val Glu Gly Asn Arg Gly Pro Gln Ala Val Lys Val Glu 50 55 60 Ala Ile Ala 65 7052196DNAOryza sativaRice GOS2 gene promoter 705aatccgaaaa gtttctgcac cgttttcacg tcctaactaa caatataggg aacgtgtgct 60aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgt aagaaaaact 120catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300aaaaaatctt tctagctgaa ctcaatgggt aaagagagat attttttttt aaaaaaaaat 360agaatgaaga tattctgaac gtatcggcaa agatttaaac atataattat ataattttat 420agtttgtgca ttcgttatat cgcacgtcat taaggacatg tcttactcca tctcaatttt 480tatttagtaa ttaaagacaa ttgacttatt tttattattt atcttttttc gattagatgc 540aaggtactta cgcacacact ttgtgctcat gtgcatgtgt gagtgcacct cctcaataca 600cgttcaacta gcgacacatc tccaatatca ctcgcctatt taatacattt aggtagcaat 660atctgaattc aagcactcca ccatcaccag accactttta ataatatcta aaatacaaaa 720aataatttta cagaatagca tgaaaagtat gaaacgaact atttaggttt ttcacataca 780aaaaaaaaaa gaattttgct cgtgcgcgag cgccaatctc ccatattggg cacacaggca 840acaacagagt ggctgcccac agaacaaccc acaaaaaacg atgatctaac ggaggacagc 900aagtccgcaa caacctttta acagcaggct ttgcggccag gagagaggag gagaggcaaa 960gaaaaccaag catcctcctc ctcccgtcta taaattcctc cccccttttc ccctctctat 1020ataggaggca tccaagccaa gaagagggag agcaccaagg acacgcgact agcagaagcc 1080gagcgaccgc ctcctcgatc catatcttcc ggtcgagttc ttggtcgatc tcttccctcc 1140tccacctcct cctcacaggg tatgtgcctc ccttcggttg ttcttgaatt tattgttcta 1200ggttgtgtag tacgggcctt gatgttagga aaggggatct gtatctgtga tgattcctgt 1260tcttggattt gggatagagg ggttcttgat gttgcatgtt atcggttcgg tttgattagt 1320agtatggttt tcaatcgtct ggagagctct atggaaatga aatggtttag ggatcggaat 1380cttgcgattt tgtgagtacc ttttgtttga ggtaaaatca gagcaccggt gattttgctt 1440ggtgtaataa agtacatttg tttggtcctc gattctggta gtgatgcttc tcgatttgac 1500gaagctatcc tttgtttatt ccctattgaa caaaaataat ccaactttga agacggtccc 1560gttgatgaga ttgaatgatt gattcttaag cctgtccaaa atttcgcagc tggcttgttt 1620agatacagta gtccccatca cgaaattcat gaaaacagtt ataatcctca ggaacagggg 1680attccctgtt cttccgattt gctttagtcc cagaattttt tttcccaaat atcttaaaaa 1740gtcactttct ggttcagttc aatgaattga ttgctacaaa taatgctttt atagcgttat 1800cctagctgta gttcagttta taggtaatac ccctatagtt tagtcaggag aagaacttat 1860ccgatttctg atctccattt ttaattatat gaaatgaact gtagcataag cagtattcat 1920ttggattatt ttttttatta gctttcaccc cttcattatt ctgagctgaa agtctggcat 1980gaactgtcct caattttgtt ttcaaattca catcgattat ctatcgatta tcctcttgta 2040tctacctgta gaagtttctt tttggttatt ccttgactgc ttgattacag aaagaaattt 2100atgaagctgt aatcgggata gttatactgc ttgttcttat gattcatttc ctttgtgcag 2160ttcttggtgt agcttgccac tttcaccagc aaagtt 21967061748DNAArtificial Sequencesynthetic sequence, 35S enhanced rice actin promoter 706ctcgaggtca ttcatatgct tgagaagaga gtcgggatag tccaaaataa aacaaaggta 60agattacctg gtcaaaagtg aaaacatcag ttaaaaggtg gtataaagta aaatatcggt 120aataaaaggt ggcccaaagt gaaatttact cttttctact attataaaaa ttgaggatgt 180ttttgtcggt actttgatac gtcatttttg tatgaattgg tttttaagtt tattcgcttt 240tggaaatgca tatctgtatt tgagtcgggt tttaagttcg tttgcttttg taaatacaga 300gggatttgta taagaaatat ctttagaaaa acccatatgc taatttgaca taatttttga 360gaaaaatata tattcaggcg aattctcaca atgaacaata ataagattaa aatagctttc 420ccccgttgca gcgcatgggt attttttcta gtaaaaataa aagataaact tagactcaaa 480acatttacaa aaacaacccc taaagttcct aaagcccaaa gtgctatcca cgatccatag 540caagcccagc ccaacccaac ccaacccaac ccaccccagt ccagccaact ggacaatagt 600ctccacaccc ccccactatc accgtgagtt gtccgcacgc accgcacgtc tcgcagccaa 660aaaaaaaaag aaagaaaaaa aagaaaaaga aaaaacagca ggtgggtccg ggtcgtgggg 720gccggaaacg cgaggaggat cgcgagccag cgacgaggag cttaggcctc atcgttgaag 780atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 840aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 900taagggatga cgcacaatcc cactatcctt cgaggcctca tcgttgaaga tgcctctgcc 960gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt 1020ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac 1080gcacaatccc actatccttc gaagctccct ccctccgctt ccaaagaaac gccccccatc 1140gccactatat acataccccc ccctctcctc ccatcccccc aacccttcta gaaccatctt 1200ccacacactc aagccacact attggagaac acacagggac aacacaccat aagatccaag 1260ggaggcctcc gccgccgccg gtaaccaccc cgcccctctc ctctttcttt ctccgttttt 1320ttttccgtct cggtctcgat ctttggcctt ggtagtttgg gtgggcgaga ggcggcttcg 1380tgcgcgccca gatcggtgcg cgggaggggc gggatctcgc ggctggggct ctcgccggcg 1440tggatccggc ccggatctcg cggggaatgg ggctctcgga tgtagatctg cgatccgccg 1500ttgttggggg agatgatggg gggtttaaaa tttccgccgt gctaaacaag atcaggaaga 1560ggggaaaagg gcactatggt ttatattttt atatatttct gctgcttcgt caggcttaga 1620tgtgctagat ctttctttct tctttttgtg ggtagaattt gaatccctca gcattgttca 1680tcggtagttt ttcttttcat gatttgtgac aaatgcagcc tcgtgcggag cttttttgta 1740ggtagaag 17487074PRTArtificialSynthetic 707Ala Leu Glu Ala7084PRTArtificialSynthetic 708Asp Val Leu Pro

Claims

1.-12. (canceled)

13. A recombinant DNA comprising a promoter that functions in plants operably linked to a polynucleotide encoding a protein, wherein said protein is a variant of a Bacillus subtilis cspB protein having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1 and comprising at least one mutation in a DOT4, region of residues 40 to 44 of SEQ ID NO:1.

14. The recombinant DNA of claim 13, wherein said protein comprises a protein with an amino acid sequence selected from the group consisting of SEQ ID NO: 30, SEQ ID NO: 61-66, SEQ ID NO: 107-111, SEQ ID NO: 173-177, SEQ ID NO: 216-219, SEQ ID NO: 253-260, SEQ ID NO: 264-265, SEQ ID NO: 268, SEQ ID NO: 269, SEQ ID NO: 636-644, and SEQ ID NO: 646-649.

15. The recombinant DNA according to claim 13, wherein the promoter is selected from the group consisting of inducible promoters, constitutive promoters, temporal-regulated promoters, developmentally-regulated promoters, tissue-preferred promoters, cold enhanced promoters, cold-specific promoters, stress enhanced promoters, stress specific promoters, drought inducible promoters, water deficit inducible promoters, and tissue-specific promoters.

16.-19. (canceled)

20. A non-natural transgenic plant comprising a recombinant DNA according to claim 13.

21. A plant propagule of a transgenic plant of claim 20.

22. A plant propagule of claim 21, wherein said propagule is a seed.

23. A method for obtaining an abiotic-stress resistant plant comprising planting of a seed of claim 22.

24.-28. (canceled)

29. A processed plant product comprising a detectable amount of the polynucleotide that is operably linked to the promoter of the recombinant DNA of claim 13.

30. The processed plant product of claim 29, wherein said product comprises a feed, a meal, a flour, an extract, or a homogenate, wherein said feed, meal, flour, extract, or homogenate is obtained from at least one plant part.

31. The processed plant product of claim 30, wherein said plant part is a stem, a leaf, a root, a flower, a tuber, or a seed.

32. The processed product of claim 29, wherein said processed plant product comprises a composition enriched for one or more protein(s), one or more monosaccharide(s), one or more disaccharide(s), one or more polysaccharides, or one or more fatty acid(s).

33.-37. (canceled)

38. The recombinant DNA of claim 13, wherein said protein comprises a protein with the amino acid sequence of SEQ ID NO:30.

39. The transgenic plant of claim 20, wherein said recombinant DNA encodes a protein comprising the amino acid sequence of SEQ ID NO:30.

40. The plant propagule of claim 22, wherein said recombinant DNA encodes a protein comprising the amino acid sequence of SEQ ID NO:30.

41. The plant propagule of claim 22, wherein the seed is selected from the group consisting of a soybean, a corn, a canola, a rice, a cotton, a barley, an oat, an alfalfa, a turf grass, a cotton, and a wheat seed.