Hb-egf Inhibitor Derived From The R Domain Of Diphtheria Toxin For The Treatment Of Diseases Associated With The Activation Of The Hb-egf/egfr Pathway

Abstract

A ligand recombinant protein inhibiting HB-EGF (Heparin-Binding Epidermal Growth Factor like), from the R domain of diphtheria toxin, which can be used for the treatment and diagnosis of diseases involving the activation of the HB-EGF/EGFR pathway.

Description

[0001] The present invention relates to a ligand recombinant protein inhibiting HB-EGF (Heparin-Binding Epidermal Growth Factor-like growth factor), derived from the R domain of diphtheria toxin, of use for the treatment and diagnosis of diseases involving the activation of the HB-EGF/EGFR pathway.

[0002] HB-EGF is a growth factor expressed at the surface of the cells of the organism in the form of a precursor membrane protein, pro-HB-EGF, which is the natural diphtheria toxin receptor.

[0003] Diphtheria toxin (DT) is an exotoxin of 535 amino acids (SEQ ID NO: 1) composed of a fragment A (N-terminal) and of a fragment B (C-terminal). Fragment A comprises the catalytic domain (C or DTA/DT-A; residues 1 to 193) and fragment B comprises the translocation domain (T; N-terminal; residues 202 to 378), and the cell receptor-binding domain (R or DTR; C-terminal; residues 379-386 to 535). DT binds to the surface of cells via the binding of its R domain to pro-HB-EGF; after activation of DT by proteolytic cleavage between fragments A and B, the T domain allows translocation of fragment A into the cytoplasm. Once in the cytoplasm, the catalytic domain carried by fragment A blocks protein synthesis by inactivating the EF2 elongation factor, thus causing cell death. The study of natural or synthetic mutants of DT has shown that the binding of DT to the pro-HB-EGF receptor is abolished by the substitution of residue S508 (S508F) or of residues of the loop of the region K516-F530 of DTR (K516A, K516E, F530A, F530S and S525F), but not by the deletion of the 4 C-terminal residues (residues E532-S535; Shen et al., J. Biol. Chem., 1994, 269, 29077-29084). In addition, the natural mutant of DT comprising the G52E mutation, called CRM197, has a greatly reduced catalytic activity (Giannini et al., N.A.R., 1984, 12, 4063-4069).

[0004] During inflammatory processes involving HB-EGF, the pro-HB-EGF membrane protein is cleaved by a protease and HB-EGF is released from the cell surface, in the form of a secreted protein. It then binds in an autocrine or paracrine manner to the ErbB1 (HER1 or EGFR) and ErbB4 (HER4) subunits of the EGF receptor family (EGFR family or EGFR), expressed in virtually all the tissues of the organism. There are at least ten EGFR ligands in addition to HB-EGF, namely EGF, TGF-alpha, amphiregulin, betacellulin, epigen, epiregulin and neuregulins -1, -2, -3 and -4. It is the local context which determines the stimulation of EGFR by one or the other of these ligands.

[0005] The activation of the HB-EGF/EGFR pathway which is associated with increased expression of pro-HB-EGF and/or the release of HB-EGF leads to deleterious cell proliferation responsible for numerous pathological conditions:

[0006] rapidly progressive glomerulonephritis (RPGN) during which the proliferation of the glomerular renal cells (podocytes) results in kidney destruction (Feng et al., J. Clin. Invest., 2000, 105, 341-350; Bollee et al., Nat. Med., 2011, 17, 1242-1250),

[0007] cancers, including ovarian cancer, endometrial cancer, breast cancer, uterine cancer (uterine adenocarcinoma), bladder cancer, stomach cancer, skin cancer (malignant melanoma), brain cancer (glioblastoma) and lung cancer (Yotsumoto et al., Biochem Biophys Res Commun, 2008, 365, 555-561; Miyamoto et al., Adv. Exp. Med. Biol., 2008, 622, 281-295; Miyamoto et al., Anticancer Res., 2009, 29, 823-830; Fu et al., Proc. Natl. Acad. Sci. U S A, 1999, 96, 5716-5721; Tsujioka et al., Anticancer. Res., 2010, 30, 3107-3112),

[0008] vasospasm associated with cerebral contusions (Chansel et al., FASEB J., 2006, 20, 1936-1938),

[0009] cardiac hypertrophy (Asakura et al., Nat. Med., 2002, 8, 35-40; Hamaoka et al., J. Biochem., 2010, 148, 55-69),

[0010] smooth muscle cell hyperplasia (Miyagawa et al., J. Clin. Invest., 1995, 95, 404-411; Hamaoka et al., J. Biochem., 2010, 148, 55-69),

[0011] pulmonary hypertension (Powell et al., Am. J. Pathol., 1993, 143, 784-793; Hamaoka et al., J. Biochem., 2010, 148, 55-69),

[0012] diabetic retinopathies, and

[0013] arterial restinosis.

[0014] Because of its involvement in an increasing number of diseases, HB-EGF represents a therapeutic target. There are currently two types of molecules available for blocking the action of HB-EGF on the EGFR, EGFR inhibitors and HB-EGF inhibitors. These molecules which are potentially usable for treating pathological conditions involving EGFR activation by HB-EGF (HB-EGF/EGFR pathway) have, however, a certain number of major drawbacks.

[0015] EGFR Inhibitors

[0016] Two types of EGFR inhibitors could theoretically be used to block EGFR stimulation by HB-EGF: reversible tyrosine kinase inhibitors which act on the EGFR, such as erlotinib, and antibodies directed against the EGFR, such as cetuximab (Ciardiello and Tortora, N. Engl. J. Med., 358, 1160-1174; WO 2008/053270).

[0017] However, these molecules which act on the tyrosine kinase of the EGFR (small molecules) or on the EGFR itself (monoclonal antibodies) are not specific for the HB-EGF/EGFR pathway, given that they block the activation of the EGFR irrespective of the origin of its stimulation, independently of the ligand activating it and irrespective of this ligand. Because of their lack of specificity, EGFR inhibitors produce significant side effects. By way of example, mention may be made of the following side effects reported, respectively, for erlotinib and cetuximab: neutropenia, thrombocytopenia, anemia, edema, nausea, vomiting, headaches, pruritus and musculoskeletal pain; fever, shivering, urticaria, pruritus, skin rash, hypotension, bronchospasm, dyspnea, edema, confusion, anaphylactic shock and cardiac arrest.

[0018] Furthermore, antibodies are too big (150 kDa) to reach their target in the case of RPGN given that the EGFR stimulated by HB-EGF is expressed by the podocytes in the kidney glomeruli and that the glomerular filtration threshold is approximately 68 kDa. Likewise, the penetration of antibodies into solid tumors is limited owing to their size.

[0019] HB-EGF Inhibitors

[0020] In mice, knockout of the pro-HB-EGF gene prevents the triggering of induced RPGN (Bollee et al., Nat. Med., 2011, 17, 1242-1250), thus justifying the advantage of developing a ligand inhibiting HB-EGF and pro-HB-EGF for the treatment of pathological conditions involving the activation of the HB-EGF/EGFR pathway.

[0021] There are currently two types of HB-EGF inhibitors potentially usable for blocking EGFR stimulation by HB-EGF: antibodies directed against HB-EGF (WO 2009/040134; WO 2011/21381; WO 2009/72628; EP 2221374; EP 2281001; EP 2093237; EP2078731; EP 2039704; WO 2008/053270) and CRM197 (U.S. Pat. No. 7,700,546; US 2006/0270600).

[0022] The antibodies directed against HB-EGF are more specific than the EGFR inhibitors given that they block only the activation of the HB-EGF/EGFR pathway, but they have the same drawbacks as the anti-EGFR antibodies, linked to their excessive size.

[0023] CRM197 is a natural ligand of HB-EGF capable of blocking its binding to the EGFR. It is currently used in clinical trials to block HB-EGF in order to combat ovarian cancer (Koshikawa et al., Cancer Sci., 2011, 102, 111-116; Tsujioka et al., Anticancer Res., 2011, 31, 2461-2465).

[0024] However, CRM197 has drawbacks in terms of residual toxicity, size, affinity, immunogenicity and antigenicity.

[0025] The residual toxicity of CRM197 is 10.sup.6 times lower than that of the wild-type diphtheria toxin (Kageyama et al., J. Biochem., 2007, 142, 95-104). This is, however, not insignificant since the wild-type toxin is extremely powerful with a lethal dose 50 (LD50) of 5 pM in primate cell culture. CRM197 is therefore toxic at micromolar (.mu.M) doses. This can present a risk if it is administered to humans at high dose (Kageyama et al., J. Biochem., 2007, 142, 95-104).

[0026] CRM197 has a molecular weight of 58 kDa, passing the glomerular filtration threshold with difficulty. It cannot therefore be used to treat RPGN or other pathological conditions such as solid tumors, where tissue penetration is an important factor for therapeutic efficacy.

[0027] CRM197 has a medium affinity for HB-EGF (Kd=27 nM; Brooke et al., Biochem. Biophys. Res. Commun., 1998, 248, 297-302). This affinity is lower than that of a good therapeutic antibody.

[0028] CRM197 is very immunogenic. It differs from diphtheria toxin only by one residue (G52E). It therefore carries all of the CD4 T epitopes of diphtheria toxin, or almost, if the mutation affects a T epitope. As it happens, western populations are vaccinated against diphtheria toxin. Treating patients with CRM197 amounts to giving a vaccination booster. As early as the first injection, CRM197 can cause the reactivation of memory CD4 T cells and can restimulate the production of circulating antibodies. A few days after the first administration of CRM197, these antibodies should neutralize the protein and make the treatment ineffective.

[0029] CRM197 is very antigenic since it has virtually all the B epitopes of diphtheria toxin. Since the western population is vaccinated against diphtheria toxin, a significant fraction of individuals have circulating antibodies capable of neutralizing CRM197 as early as the first administration. This imposes the use of massives doses of CRM197 in order to obtain a therapeutic effect, while considerably increasing the risks of side effects (toxicity, anaphylactic shock, etc.)

[0030] Consequently, there is a real need to have new HB-EGF inhibitors which have a smaller size, a better affinity for HB-EGF, and a reduced antigenicity, immunogenicity and toxicity, compared with CRM197.

[0031] Up until now, it has not been possible to produce the isolated DTR domain, directly, in recombinant form, with a high yield, and without using detergents or chaotropic or denaturing agents to extract the recombinant protein from the transformed cells.

[0032] Indeed, in order to stabilize the structure of the isolated DTR domain, and to improve the extraction and purification of this domain from the transformed bacteria, it is essential to fuse the DTR domain to a protein (glutathione-S-transferase, GST) or to a protein domain (ZZ domain derived from S. aureus protein A (Lobeck et al., Infect. Immun., 1998, 66, 418-423; Shen et al., J. Biol. Chem., 1994, 269, 29077-29084). In addition, mutants of the loop of region 516-530 of DTR have been produced using the GST-DTR fusion; these DTR mutants are incapable of binding to the pro-HB-EGF receptor (Shen et al., 1994).

[0033] In the abovementioned two cases, the recombinant protein produced in E. coli is not an isolated DTR domain, but a GST-DTR or ZZ-DTR fusion protein. In the case of the GST-DTR fusion protein, the production yields are mediocre and it is essential to use a detergent (1% Triton X-100) to extract the fusion protein from the transformed bacteria. Furthermore, in order to produce a therapeutic protein, the fusion partner must be removed, thereby making the production process much more complex and even further reducing yields.

[0034] The inventors have constructed mutants of the isolated DTR domain, devoid of fusion-partner sequences. These mutants of the isolated DTR domain are expressed directly and in a large amount in the form of a soluble recombinant DTR protein of small size (approximately 17500 Da) with affinity for pro-HB-EGF and HB-EGF. These DTR mutants have been modified to produce improved recombinant DTR proteins which, surprisingly, have both a reduced antigenicity and immunogenicity and a considerably increased affinity, compared with CRM197.

[0035] Consequently, a subject of the present invention is a recombinant protein comprising an amino acid sequence having at least 70% similarity with residues 380 to 535 of the amino acid sequence SEQ ID NO: 1 which correspond to the R domain of diphtheria toxin, said sequence comprising the substitution of at least one, preferably at least two, of the residues Y380, P382, Q387, P388 and/or L390 of said R domain with another amino acid selected from the group consisting of: S, T, N, C, Y, Q, R, K, H, D and E, and said sequence being devoid, at the N- or C-terminal end of said R domain, of the sequence of the T domain or of the C domain and of the T domain of diphtheria toxin and of the sequence of a protein or of a protein domain capable of improving the stability or the purification of said R domain, and said recombinant protein being a ligand inhibiting HB-EGF.

[0036] The invention provides a therapeutic recombinant protein that is a ligand of HB-EGF and of pro-HB-EGF, which has the following advantages:

[0037] it has a greatly increased solubility compared with the wild-type form DTR.sub.WT. The substitution of at least one, preferably at least two, of the residues Y380, P382, Q387, P388 and/or L390 with a different hydrophilic, polar or charged amino acid residue as defined above makes it possible to considerably increase the solubility of the DTR protein in aqueous solution. Thus, the recombinant protein according to the invention can be extracted from the host cells and purified without using detergents or chaotropic or denaturing agents. By comparison, the wild-type DTR protein (DTR.sub.WT) produced in the same expression system is insoluble and not solubilized using detergents compatible with therapeutic use. The DTR.sub.WT protein is solubilized in the presence of 0.5% of sarkosyl or sodium dodecyl sulfate, which are detergents that are incompatible with therapeutic use at these concentrations;

[0038] it is much easier to produce in recombinant form than DTR.sub.WT. It is produced directly, without using a fusion partner for improving the stability or the purification of the R domain. It is produced in E. coli according to standard fermentation procedures, in a folded soluble form, and in a large amount after final purification (several tens of mg/l of culture under nonoptimized laboratory conditions);

[0039] it has an affinity for HB-EGF and pro-HB-EGF which is at least 10 times greater than that of CRM197. Surprisingly, although the wild-type DTR protein (DTR.sub.WT) has an affinity for HB-EGF and pro-HB-EGF which is 2-fold lower than that of CRM197, the mutant DTR proteins according to the invention have an affinity for HB-EGF and pro-HB-EGF that is considerably higher than that of CRM197; the proteins called DTR1, DTR3 and DTR8 have, respectively, an affinity which is 60, 300 and 1400 times higher than that of CRM197. This means that DTR8 could be used at doses much lower than CRM197 for the same therapeutic effect. As a result, the risks of side effects, linked in general to the existence of low-affinity binding with other proteins or ligands of the organism, are therefore considerably reduced by comparison with CRM197. These interactions are eliminated by using low doses, of the order of one pM, which should be the case for DTR8;

[0040] it has a reduced immunogenicity compared with CRM197 and with DTR.sub.WT. Ten of the 26 T epitopes identified in DTR.sub.WT have been deleted from the DTR8 protein by site-directed mutagenesis, including 7 among 9 immunodominant epitopes;

[0041] it has a greatly reduced antigenicity compared with CRM197 and with DTR.sub.WT. The sera of individuals vaccinated against diphtheria toxin preferentially recognize the catalytic domain of said toxin. This domain is present in the CRM197 molecule and absent from the mutated DTR protein according to the invention. Moreover, the mutated DTR protein according to the invention is not as well recognized as DTR.sub.WT by the antibodies of vaccinated subjects which recognize DTR.sub.WT;

[0042] it blocks a pathway of EGFR (ErbB1 and ErbB4) activation by HB-EFG much more specifically than the commercial EGFR inhibitors (therapeutic antibodies and small molecules), as a result presenting potentially much less risk of side effects;

[0043] it is small in size; the proteins of SEQ ID NOs: 2 to 9 have a sequence of 158 amino acids and an MW of approximately 17 500 Da, i.e. a size 3.4 times smaller than that of CRM197 and 8.8 times smaller than that of the anti-HB-EGF antibodies currently used in clinical protocols for blocking the HB-EGF pathway. As a result of its small size, the recombinant protein according to the invention is more effective in the treatment of pathological conditions associated with the activation of the HB-EGF pathway, in particular RPGN or ovarian cancer, since it diffuses more readily in the tissues, in particular tumors, and is capable of penetrating into kidney glomeruli.

[0044] In addition, because of its high affinity for HB-EGF and pro-HB-EGF, the protein according to the invention can also be used for the diagnosis of diseases involving the activation of the HB-EGF/EGFR pathway.

[0045] Definitions

[0046] In the present application, the term "DTR" or "DTR domain" is intended to mean the R domain of diphtheria toxin which corresponds to residues 380-385 to 531-535 of the amino acid sequence of wild-type diphtheria toxin (SEQ ID NO: 1).

[0047] The expression "DTR protein" denotes a recombinant protein comprising an isolated DTR domain, i.e. which is devoid, at its N- or C-terminal end, of the sequence of the T domain or of the C domain and of the T domain of diphtheria toxin and of the sequence of a protein or of a protein domain capable of improving the stability or the purification of said R domain. In addition, the purification of the R domain includes the extraction and the purification of said domain, given that it is produced in the form of a recombinant protein.

[0048] The recombinant protein according to the invention which comprises at least one, preferably at least two, substitution(s) as defined above is denoted mutant DTR protein, mutated DTR protein or DTR.sub.n protein where n is an integer, as opposed to the wild-type DTR recombinant protein (DTR.sub.WT) which does not comprise this substitution.

[0049] The amino acids are denoted using the one-letter code.

[0050] The similarity of an amino acid sequence compared with a reference sequence is assessed according to the percentage of amino acid residues which are identical or which differ via conservative substitutions, when the two sequences are aligned so as to obtain the maximum correspondence between them. When only the identical residues are taken into account and the percentage of identical residues is determined, reference is then made to the identity of said amino acid sequence relative to the reference sequence. For the purpose of the present invention, the expression "conservative substitution in the amino acid sequence of a protein" is intended to mean the substitution of one amino acid with another natural or synthetic amino acid which has similar chemical or physical properties (size, charge or polarity), which do not have a deleterious effect on the biological activity of the protein. Thus, two amino acid sequences of a protein are similar when they differ from one another by the substitution of an amino acid, or the deletion and/or insertion of an amino acid or of a small number of amino acids (less than 5) at positions which do not have a deleterious effect on the biological activity of said protein. The percentage similarity or identity can be calculated by those skilled in the art using sequence comparison software, such as, for example, that of the BLAST software series (Altschul et al., NAR, 1997, 25, 3389-3402). The BLAST programs are implemented using the default parameters, on a comparison window consisting of residues 380 to 535 of the amino acid sequence SEQ ID NO: 1.

[0051] Unless otherwise indicated, the term "HB-EGF" denotes both the membrane precursor (pro-HB-EGF) and the secreted form of HB-EGF.

[0052] The activity of ligand inhibiting HB-EGF refers to the capacity to bind to pro-HB-EGF and to HB-EGF and to the inhibition of various measurable biological phenomena, such as:

[0053] the inhibition of the toxic effect of diphtheria toxin on human or simian cells such as Vero cells,

[0054] the inhibition of the proliferating activity of HB-EGF on cells expressing the ErbB1 and/or ErbB4 subtypes of the EGFR and the growth of which is HB-EGF-dependent, such as a murine lymphoid cell line Ba/F3 transfected with the EGFR gene.

[0055] The recombinant protein according to the invention comprises an isolated DTR domain, devoid, at its N- or C-terminal end, of one of the following sequences: (1) the sequence of the T domain or of the C domain and of the T domain of diphtheria toxin, and (2) the sequence of a fusion partner capable of improving the stability of the DTR domain, such as the sequence of gluthatione-S-transferase (GST), or capable of improving the extraction and the purification of the DTR domain from the transformed bacteria, such as the sequence of an immunoglobulin binding domain derived from S. aureus protein A (ZZ domain).

[0056] The recombinant protein according to the invention generally comprises an isolated DTR domain which extends from residues 380 to 535 of the sequence SEQ ID NO: 1 and comprises the substitution of one or more, preferably at least two, of the residues Y380, P382, Q387, P388 and/or L390 with a hydrophilic, polar or charged amino acid residue as defined above. However, it may also comprise a slightly shorter isolated DTR domain, the N-terminal end of which is in position 381 to 385 and the C-terminal end of which is in position 531 to 534 of the sequence SEQ ID NO:1. When the N-terminal sequence of DTR is in position 381 or 382, the recombinant protein according to the invention comprises the substitution of at least one of the residues P382, Q387, P388 and/or L390. When the N-terminal sequence of DTR is in position 383, 384 or 385, the recombinant protein according to the invention comprises the substitution of at least one of the residues Q387, P388 and/or L390.

[0057] The recombinant protein according to the invention optionally comprises a methionine (M) at its N-ter end and/or additional sequences at the N- and/or C-terminal of the DTR domain. Indeed, the precursor of said recombinant protein comprises an N-terminal methionine which can be cleaved by post-translational modifications, such that it is absent in the mature recombinant protein.

[0058] For therapeutic applications, the protein according to the invention consists successively of: (1) a sequence of 1 to 10 amino acids, preferably 1 to 5 amino acids, preferably of 1 or 2 amino acids, the sequence of the precursor and optionally that of the mature protein beginning with a methionine (M), for example an MG sequence, and (2) a mutated DTR domain as defined above. Such a therapeutic protein has a small size, of approximately 145 to 200 amino acids, preferably of approximately 145 to 175 amino acids, preferably of approximately 160 amino acids.

[0059] For diagnostic applications, the protein according to the invention is labeled, in particular with a peptide tracer which can be detected by measuring an appropriate signal. The peptide tracer is in particular a tag recognized by an antibody, a fluorescent protein, or a peptide which binds at least one Technetium 99 atom. The label is in the N-ter or C-ter position of the protein, i.e. fused, directly or by means of a peptide spacer, respectively to the N-ter or C-ter end of the DTR domain. Such a protein has a size of approximately 145 to 500 amino acids, preferably of approximately 145 to 300 amino acids, preferably of approximately 145 to 200 amino acids.

[0060] The invention encompasses the modified recombinant proteins derived from the previous one by the introduction of any modification at the level of one or more amino acid residue(s), of the peptide bond or of the ends of the recombinant protein, provided that said modified protein retains good affinity and an inhibitory activity with respect to HB-EGF and to pro-HB-EGF. These modifications which are introduced into the proteins by conventional methods known to those skilled in the art include, in a nonlimiting manner: the substitution of an amino acid with a synthetic amino acid (D amino acid or amino acid analog); the addition of a chemical group (lipid, oligosaccharide or polysaccharide) at the level of a reactive function, in particular of the side chain R; the modification of the peptide bond (--CO--NH--), in particular via a bond of the retro or retro-inverso type (--NH--CO--) or a bond other than the peptide bond; cyclization; fusion (by genetic engineering) to a peptide or a protein of interest or coupling (via chemical bonding) to a molecule of interest, in particular a labeling agent or tracer which is detectable by measuring a signal.

[0061] According to one advantageous embodiment of said protein, it comprises at least one substitution selected from the group consisting of Y380K, Y380E, P382T, Q387E, Q387K, P388T, L390T and L390N.

[0062] According to one advantageous arrangement, said substitution(s) is (are) selected from the group consisting of Y380K, Y380E, Q387K, Q387E and L390T. Preferably, said substitution(s) is (are) selected from the group consisting of Y380K, Q387E and L390T.

[0063] According to another advantageous arrangement, said protein comprises at least two or three of said substitutions.

[0064] Preferably, said protein comprises at least the substitutions Y380K and L390T, Y380K and Q387E, Y380E and L390T or Y380E and Q387K, preferably at least the substitutions Y380K and L390T or Y380K and Q387E. For example, said protein comprises one of the following substitution combinations: Y380K and L390T; Y380K, Q387E and L390T; Y380K, Q387E, P388T and L390T; Y380K, Q387E, P382T and L390T; Y380E, Q387K, P382T and L390T. Preferably, it is a protein which comprises or consists of one of the sequences SEQ ID NOs: 2 to 6.

[0065] Preferably, said protein comprises at least the substitutions Y380K and L390T. For example, said protein comprises or consists of one of the sequences SEQ ID NOs: 2 to 5.

[0066] Even more preferably, said protein comprises the substitutions Y380K, Q387E and L390T. For example, said protein comprises or consists of one of the sequences SEQ ID NOs: 3 to 5, preferably the sequence SEQ ID NO: 3.

[0067] According to another advantageous embodiment of said protein, it comprises in addition the substitution A395T. The addition of the substitution produces an additional increase in the yield of soluble protein. Preferably, said protein comprises the sequence SEQ ID NO: 7; this protein called DTR1 comprises the substitutions Y380K, Q387E, L390T and A395T. The HB-EGF-binding affinity of the DTR1 protein is increased by a factor 60 compared with that of CRM197 and by a factor of 130 compared with that of the DTR.sub.WT protein solubilized using 0.5% of sarkosyl detergent.

[0068] According to yet another advantageous embodiment of said protein, it also comprises at least one of the substitutions F389Y and/or G510A. The addition of one of these substitutions increases the HB-EGF-binding affinity of the DTR protein. In addition, the combination of the two substitutions produces an additional increase in the HB-EGF-binding affinity, compared with the increase obtained with a single substitution. Preferably, said protein comprises or consists of the sequence SEQ ID NO: 8; this protein called DTR3 comprises the substitutions Y380K, Q387E, L390T, A395T, F389Y and G510A. The affinity of the DTR3 protein for HB-EGF is increased by a factor of 5 compared with that of DTR1 and by a factor of 300 compared with that of CRM197.

[0069] According to another advantageous embodiment of said protein, it also comprises at least one substitution selected from the group consisting of N399K, V452T, T517E, V483Q, H492E, S494K, T436H and E497D. Said protein advantageously comprises at least 3, preferably at least 5, of said substitutions.

[0070] Preferably, said protein comprises the substitutions N399K, V452T, T517E, V483Q, H492E and S494K. These mutations have made it possible to delete 10 of the 26 CD4 T epitopes identified in DTR.sub.WT. Among these 10 epitopes are 7 of the 9 epitopes predicted as being immunodominant epitopes of DTR.sub.WT. The capacity of the resulting protein to induce an immune response of CD4 type, i.e. an antibody-producing response, is thus considerably reduced compared with that of DTR.sub.WT. Notably and unexpectedly, the addition of the six mutations intended to reduce the immunogenicity of DTR has contributed to increasing its affinity for HB-EGF.

[0071] Preferably, said protein comprises or consists of the sequence SEQ ID NO: 9; this protein called DTR8 comprises the substitutions Y380K, Q387E, L390T, A395T, F389Y, G510A, N399K, V452T, T517E, V483Q, H492E and S494K. It has a molecular weight of 17458 Da.

[0072] The affinity of the DTR8 protein for HB-EGF is increased by a factor of 3 compared with that of DTR3 and by a factor of 1400 compared with that of CRM197. In addition, DTR8 is at least 300 times more effective than CRM197 in terms of binding to secreted HB-EGF and inhibiting the HB-EGF/EGFR pathway.

[0073] The antigenicity of the DTR8 protein is greatly reduced compared with that of CRM197. Indeed, most of the antibodies present in adults vaccinated against DT (obligatory vaccination) are directed against the catalytic domain of DT. Surprisingly and unexpectedly, the antigenicity of the DTR8 protein is reduced compared with that of DTR1, and therefore of DTR.sub.WT.

[0074] The protein according to the invention may comprise additional substitutions, in particular of residues located at the surface of the DTR domain, so as to even further reduce its antigenicity.

[0075] According to another advantageous embodiment of said protein, it comprises an amino acid sequence which has at least 80% similarity with residues 380 to 535 of the sequence SEQ ID NO: 1, preferably at least 85%, preferably at least 90%. According to one advantageous arrangement of said protein, it comprises an amino acid sequence which has at least 70% identity, preferably at least 80%, 85% or 90% identity, with residues 380 to 535 of the sequence SEQ ID NO: 1.

[0076] According to another advantageous embodiment of said protein, it is labeled with a detectable tracer. The means and the techniques for labeling proteins are well known to those skilled in the art and include radioactive, magnetic or fluorescent labeling which can be carried out directly or indirectly. The direct labeling agents are, in particular, radioactive isotopes such as tritium (.sup.3H), iodine (.sup.125I) and technetium (.sup.99mTc) or luminescent (radioluminescent, chemoluminescent, bioluminescent, fluorescent or phosphorescent) compounds such as fluorophores, for instance, in a nonlimiting manner, AlexaFluor.RTM., FITC and Cyanine 3, and fluorescent proteins such as GFP and its derivatives. The indirect labeling agents include, in particular, enzymes and epitope tags recognized by a specific antibody. The labeling is in particular carried out by: (1) grafting a fluorophore onto a reactional group such as a reactional amine of a lysine residue, (2) incorporating a reactional group (free cysteine) by chemical synthesis or recombinant production, then using this group to graft a fluorophore, (3) directly incorporating a fluorophore, in the N- or C-terminal position, by chemical synthesis, (4) directly incorporating a fluorescent protein or an enzyme by production of a fusion protein. Such labeled proteins are in particular used as a reagent for the diagnosis, in vitro or in vivo (by imaging), of diseases associated with the activation of the HB-EGF/EGFR pathway or as a tool for studying this activation pathway. The DTR protein can be labeled directly by covalent coupling of technetium (.sup.99mTc) or of a fluorescent tracer; the fluorescent tracer is in particular coupled to one of the lysine residues of the DTR protein.

[0077] The recombinant protein of the present invention can be produced by means of a method in which an expression vector comprising a polynucleotide encoding said protein, functionally linked to the regulatory elements allowing its expression in the chosen host, is transferred into a host cell which is placed in culture under conditions which allow the expression of said protein. The protein produced is then recovered and purified. The purification methods used are known to those skilled in the art. The recombinant protein obtained can be purified from cell lysates or extracts, or from the culture medium supernatant, by means of methods used individually or in combination, such as fractionation, chromatography methods, or immunoaffinity techniques using specific monoclonal or polyclonal antibodies. The recombinant protein obtained is soluble.

[0078] A subject of the present invention is also an isolated polynucleotide encoding said recombinant protein. Said polynucleotide advantageously comprises a coding sequence optimized for expression in the host cell in which the protein of the invention is produced. Preferably, said polynucleotide comprises the sequence SEQ ID NO: 10, 12 or 14 which is optimized for expression in E. coli and encodes respectively the DTR1, DTR3 and DTR8 recombinant protein. The polynucleotide of the invention is a DNA or an RNA prepared using the conventional chemical synthesis or molecular biology methods known to those skilled in the art.

[0079] A subject of the present invention is also a recombinant cloning and/or expression vector comprising said polynucleotide. Preferably, said vector is an expression vector comprising said polynucleotide functionally linked to the regulatory sequences which allow the expression of the protein of the invention in the cell host used for the production of said protein (promoter, enhancer, initiation codon (ATG), codon stop, transcription termination signal). The vector, which may be a replicating or integrating vector, in particular a plasmid or a viral vector, is prepared according to the methods commonly used by those skilled in the art.

[0080] A subject of the present invention is also a host cell transiently or stably modified with a recombinant vector as defined above. These cells may be obtained by introducing a recombinant vector as defined above into a eukaryotic or prokaryotic host cell using standard methods known to those skilled in the art, such as electroporation. Examples of host cells include, in particular, mammalian cells, insect cells, bacteria such as E. coli and yeasts.

[0081] A subject of the present invention is also a pharmaceutical composition comprising at least one recombinant protein or one recombinant expression vector as defined above and a pharmaceutically acceptable vehicle.

[0082] The pharmaceutical composition comprises an effective dose of protein or of vector for obtaining a therapeutic effect on diseases associated with activation of the HB-EGF/EGFR pathway, as defined above. Generally, a therapeutically effective amount ranging from approximately 0.1 .mu.g to approximately 100 mg, preferably from 10 .mu.g to 10 mg, can be administered to human adults. The pharmaceutically acceptable vehicles are those conventionally used. The composition is in a galenical form suitable for a chosen administration: injectable sterile solution, powder, tablets, gel capsules, suspension, syrup or suppositories, which are prepared according to standard protocols. The administration may be subcutaneous, intramuscular, intravenous, intradermal, intraperitoneal, oral, sublingual, rectal, vaginal, intranasal, by inhalation or by transdermal application.

[0083] The modes of administration, the dosage regimens and the galenical forms of the pharmaceutical compositions according to the invention may be determined in the usual way by those skilled in the art, in particular according to the criteria generally taken into account for establishing a therapeutic treatment suitable for a patient (generally a human individual and optionally a non-human mammal), for instance age, body weight, seriousness of the patient's general condition, tolerance of the treatment, and side effects observed.

[0084] A subject of the present invention is also a recombinant protein as defined above, as a medicament.

[0085] The present invention also relates to a recombinant protein as defined above, for use in the treatment of diseases associated with activation of the HB-EGF/EGFR pathway.

[0086] A subject of the present invention is also the use of a recombinant protein as defined above, for preparing a medicament intended for the treatment of diseases associated with activation of the HB-EGF/EGFR pathway.

[0087] A subject of the present invention is also a method for treating diseases associated with activation of the HB-EGF/EGFR pathway, comprising the administration of a pharmaceutical composition as defined above, to an individual. The administration is carried out according to an appropriate mode and an appropriate rate as defined above.

[0088] Preferably, said diseases are selected from the group consisting of: rapidly progressive glomerulonephritis (RPGN), cancers, in particular ovarian cancer, endometrial cancer, breast cancer, uterine cancer (uterine adenocarcinoma), bladder cancer, stomach cancer, skin cancer (malignant melanoma), brain cancer (glioblastoma) and lung cancer, vasospasm associated with cerebral contusions, cardiac hypertrophy, smooth muscle cell hyperplasia, pulmonary hypertension, diabetic retinopathies and arterial restenosis.

[0089] A subject of the present invention is also the use of a labeled protein as defined above, for the in vitro or in vivo diagnosis of a disease associated with activation of the HB-EGF/EGFR pathway.

[0090] The invention also relates to a method for the in vitro diagnosis of a disease associated with activation of the HB-EGF/EGFR pathway, which comprises bringing a biological sample into contact with a labeled protein as defined above, under conditions which allow the formation of a specific complex between said labeled protein and HB-EGF and/or pro-HB-EGF, and detecting said labeled protein/HB-EGF complexes, by any appropriate means.

[0091] The biological sample is in particular a biopsy sample (kidney, tumor, smooth muscle, heart, lung, vessels, retina), serum sample or urine sample.

[0092] A subject of the present invention is also the use of a labeled protein as defined above, for the detection, in vitro or in vivo, of HB-EGF.

[0093] A subject of the present invention is also a method for detecting HB-EGF, in vitro and in vivo, comprising at least the following steps:

[0094] bringing cells to be analyzed into contact with a labeled protein as defined above, and

[0095] detecting the labeled cells and/or extracellular medium by any appropriate means.

[0096] This method makes it possible to determine the tissue expression profile of HB-EGF under physiological or pathological conditions or in response to an endogenous or exogenous stimulus. The detection of HB-EGF, in vivo, in the body of a mammal (cell imaging), in particular in real time, comprises a prior step of administering said protein to said mammal (parenteral injection, oral administration). The detection of HB-EGF in vivo in humans can be used to diagnose a disease associated with activation of the HB-EGF/EGFR pathway.

[0097] The labeling of the cells and of the extracellular medium containing HB-EGF is in particular fluorescent or radioactive labeling, or magnetic labeling, detectable by any technique known to those skilled in the art (fluorescence microscopy, flow cytometry, gammagraphy, magnetic resonance imaging).

[0098] A subject of the invention is also a kit for carrying out the diagnostic or detection methods as defined above, comprising a labeled protein as defined above.

[0099] In addition to the above arrangements, the invention also comprises other arrangements, which will emerge from the following description, which refers to exemplary embodiments of the subject matter of the present invention, with reference to the appended drawings in which:

[0100] FIG. 1 represents the mutations (A) and the analysis (B) of the soluble and insoluble fractions of the 5 clones selected by screening of the R1 library in order to produce a DTR protein which is more soluble than DTR.sub.WT. All the clones are soluble; the best clone is the 1 D6 clone;

[0101] FIG. 2 shows the effects of the A395T and N399D mutations on the solubility of the DTR protein produced by the 1D6 clone (denoted here G1). The best clone contains the A395T mutation;

[0102] FIG. 3 represents: (A) the inhibition of the toxic effect of increasing doses of diphtheria toxin on Vero cells by increasing doses of DTR1 and (B) the Schild regression for evaluating the Kd of DTR1 for HB-EGF according to the equation log (EC.sub.50-1)=K.sub.d log (B) where EC.sub.50 is the concentration of diphtheria toxin which gives 50% toxicity and B is the concentration of inhibitor DTR1;

[0103] FIG. 4 represents the inhibition of the toxic effect of diphtheria toxin at the concentration of 10.sup.-11 M on Vero cells by each DTR1 mutant (denoted here G2) at a concentration of 10.sup.-9 M;

[0104] FIG. 5 represents: (A) the inhibition of the toxic effect of increasing doses of diphtheria toxin on Vero cells by increasing doses of DTR8 and (B): the Schild regression for evaluating the Kd of DTR8 for HB-EGF according to the equation log (EC.sub.50-1)=K.sub.d log (B) where EC.sub.50 is the concentration of diphtheria toxin which gives 50% toxicity and B is the concentration of inhibitor DTR8;

[0105] FIG. 6 represents the inhibition of the proliferative effect of increasing doses of HB-EGF on Ba/F3 cells expressing the EGFR and the growth of which is HB-EGF-dependent, by increasing doses of DTR8 (A) and of CRM197 (B);

[0106] FIG. 7 represents the recognition of the CRM197, diphtheria toxin domain C (C) and domain T (T), DTR1 and DTR8 proteins by the antibodies present in the serum of 20 healthy donors. The titer is defined by the serum dilution which gives a fluorescence signal of 5000 arbitrary units in the ELISA assay, after subtraction of the background noise. A titer .ltoreq.20 corresponds to the background noise and therefore to the absence of measurable antibody binding. A titer of 30 was arbitrarily set for distinguishing weak antibody responses from medium or strong responses.

EXAMPLE 1

Materials and Methods

1) Cloning of Genetic Sequences, Expression and Purification of the DTR.sub.WT Protein and of its Mutants

[0107] The genetic sequence encoding the DTR domain of diphtheria toxin, covering the sequence Y380-S535 of the native toxin, was the starting point for this study. All the genetic sequences were synthesized, after optimization for expression in E. coli, by the company Geneart according to the previously determined protein sequences. These sequences were cloned into the pET28a(+) vector (Novagen) at the NcoI and SalI restriction sites. The presence of the NcoI site generates the non-native codons M and G corresponding to the N-terminal end of the recombinant protein. The sequence SEQ ID NO: 16 is the optimized nucleotide sequence which encodes the wild-type DTR protein (DTR.sub.WT) of 158 amino acids, which consists of the residues M and G followed by the residues Y380 to S535 of native diphtheria toxin. The optimized nucleotide sequences encoding the mutant and soluble forms of the DTR protein derive from the sequence SEQ ID NO: 16 by replacement of each codon to be mutated with an optimized codon encoding the mutated amino acid, as indicated in Table I:

TABLE-US-00001 TABLE I List of the optimized codons chosen for the DTR mutations Wild- Optimized type mutated Mutation codon codon Y380K tac aaa Y380E tac gaa P382T ccg acg Q387E cag gag Q387K cag aag P388T ccg acg F389Y ttt tat L390T ctg acc L390N ctg aac H391K cat aaa A395T gcg acc N399D aac gat N399K aac aaa V401Q gtg cag L427Q ctg cag L427N ctg aac L427S ctg agc T436K acc aaa T436H acc cat V452T gtg acg I457D att gat I457E att gaa R460T cgt acc A463T gcg acc A463S gcg agc A463E gcg gaa A463D gcg gat A463G gcg ggc Y478T tat acc V483D gtg gat V483E gtg gaa V483H gtg cat V483Q gtg cag A490G gcg ggc H492E cat gaa S494K agc aaa S496K agc aaa E497D gaa gat G510A ggc gcg G510M ggc atg G510Q ggc cag G510S ggc agc Q515E cag gaa T517D acc gat T517E acc gaa T521R acc cgc K522R aaa cgc

[0108] The expression of the DTR.sub.WT protein was carried out in the Escherichia coli BL21(DE3) bacterium in Terrific Broth medium in the presence of 50 .mu.g/ml of kanamycin at 37.degree. C. The induction of the protein is carried out by adding 1 mM of isopropyl .beta.-D-1-thiogalactopyranoside (IPTG). The bacteria were recovered by centrifugation at 5000 g for 45 min and lyzed in a buffer of 20 mM sodium phosphate, 500 mM NaCl, 0.25 mM phenylmethylsulfonyl fluoride (PMSF), lysozyme (0.25 mg/ml), pH 8, by passing them through a Cell Disrupter (Constant Systems). The inclusion bodies containing the protein were solubilized in a solution containing 8 M of urea in 0.1 M Tris-HCl, pH 8. The protein is purified by cation exchange chromatography on a 5 ml HiTrap.TM. SP column (GE Health Care Life Sciences) according to a buffer gradient between 8 M of urea in 0.1 M Tris-HCl, pH 8, and 8 M of urea, 1 M NaCl in 0.1 M Tris-HCl, pH 8, and then folded by means of dialysis in 2 steps, firstly against a buffer of 20 mM Tris-HCl, 50 mM NaCl, 1 mM cysteine/8 mM cystine, 0.5% sarkosyl, pH 8, and then against a buffer of 20 mM Tris-HCl, 50 mM NaCl, 0.5% sarkosyl, pH 8.

[0109] The expression of the mutant and soluble forms of the DTR protein was carried out in the Escherichia coli BL21(DE3) bacterium in 2YT medium in the presence of 50 .mu.g/mi of kanamycin. After 2 h 20 min of culture at 37.degree. C., the induction of the protein is carried out by adding 1 mM of IPTG. Then, after 4 h 30 min at 30.degree. C., the bacteria were recovered by centrifugation at 5000 g for 30 min and lyzed in a buffer of 20 mM sodium phosphate, 0.25 mM PMSF, 2 mM MgCl.sub.2, supplemented with benzonase (6.25 U/ml), pH 7.8, in a Cell Disrupter (Constant Systems). The protein, which is soluble in the lysis mixture, was purified by cation exchange chromatography on a 5 ml HiTrap.TM. SP column according to a gradient between 20 mM sodium phosphate, pH 7.8, and 20 mM sodium phosphate, 1 M NaCl, pH 7.8 (GE), and stored at -20.degree. C. in a buffer of 20 mM sodium phosphate, 500 mM NaCl, pH 7.8.

2) Screen for Selecting Soluble Mutants

[0110] Three DTR sequence libraries produced by synthesis and cloned into the pET28a(+) plasmid were transfected into the E. coli strain BL21(DE3) (Geneart). For each library, clones were subcultured in 2 or 4 96-well plates in culture medium in the presence of a selection antibiotic and of expression inducer (IPTG). After growth, the bacteria were lyzed using a Bugbuster.TM. solution (0.5.times. final concentration) (Novagen) with the addition of Lysonase.TM. (Novagen). After centrifugation of the plates for 20 min at 2700 g at 4.degree. C., the presence of inclusion bodies was evaluated well-by-well through the size of the pellet, and the soluble protein fraction was identified by analysis of the lysis supernatants on a polyacrylamide gel under denaturing conditions and with Coomassie blue staining.

3) Test for Activity of the DTR Proteins by Inhibition of Diphtheria Toxin Toxicity

[0111] Vero cells (ATCC CCL-81.TM.) were seeded into 96-well Cytostar-T.TM. scintillating plates (Perkin Elmer) at 50 000 cells per well in DMEM medium (Dulbecco's modified Eagle's minimum essential medium) supplemented with 2 mM of glutamine, 1% of a penicillin/streptomycin solution, 1% of a solution of nonessential amino acids and 10% of fetal calf serium. Variable concentrations of diphtheria toxin (Sigma) were added in duplicates. These conditions were repeated in the presence of the antagonist tested: DTR.sub.WT, mutant DTR or CRM197 protein (Sigma). After incubation for 22 h at 37.degree. C. in a 5% CO.sub.2 atmosphere, the culture medium was replaced with leucine-free medium containing 1 .mu.Ci/well of .sup.14[C]-leucine (GE Health Care Life Sciences). After incubation for 5 h at 37.degree. C. in a 5% CO.sub.2 atmosphere, the radioactivity incorporated into the cells was counted by placing the plates, covered with an adhesive film, in a MicroBeta.RTM. apparatus (Wallac).

4) Test for Activity of the DTR Proteins by Inhibition of the Proliferating Activity of HB-EGF

[0112] The test uses a murine lymphoid cell line Ba/F3 (Palacios, R & Steinmetz, M., Cell, 1985, 81, 727-734) transfected in the laboratory with the EGFR gene. This line is dependent on HB-EGF or on amphiregulin for its growth. The cells were seeded into 96-well Nunclon.TM. plates (Nunc) at 10 000 cells per well in RPMI (Roswell Park Memorial Institute) 1640 medium supplemented with 2 mM of glutamine, 1% of a penicillin/streptomycin solution, 1% of a solution of nonessential amino acids and 10% of fetal calf serum. Variable concentrations of HB-EGF or of amphiregulin were added in duplicate. These conditions were repeated in the presence of the antagonist tested: mutant DTR or CRM197 protein. After incubation for 24 h at 37.degree. C. in a 5% CO.sub.2 atmosphere, 1 .mu.Ci/well of .sup.3[H]-thymidine (GE Health Care Life Sciences) was added to each well. After incubation for 5 h at 37.degree. C. in a 5% CO.sub.2 atmosphere, the cells were aspirated on glass fiber filter (filtermat A, Wallac) using a Tomtec.RTM. apparatus. After drying of the filters, the latter were placed in a sealed bag in the presence of scintillation fluid and the radioactivity incorporated into the cells was counted in a MicroBeta.RTM. apparatus (Wallac).

5) Identification of CD4 T Epitopes of the DTR Protein

[0113] The identification of the CD4 T epitopes of the DTR protein is carried out by analysis of the DTR peptides recognized by DTR-specific CD4 T lymphocyte lines, restricted with respect to the HLA-DRB1 molecules which are predominant in caucasian phenotypes. The protocols used are those previously described in application WO 2010/076413 with the exception of the following modifications: (1) the DTR-specific CD4 T lympocyte lines are produced by coculturing of donor CD4 T lymphocytes with autologous mature dendritic cells loaded with a pool of overlapping DTR peptides, and (2) the specificity of the lines produced is analyzed by means of an ELISPOT-IFN-.gamma. assay using autologous peripheral blood mononuclear cells (PBMCs) loaded either with the peptide pool used to produce the CD4 T lymphocyte line, in order to verify the specificity of the line for DTR, or with a peptide of the pool, in order to determine the specificity of each line.

a) Isolation of the CD4 T Lymphocytes from PBMCs

[0114] Seven donors of different age and different HLA-DRB1 phenotype were selected such that all of these donors express the 8 HLA-DRB1 molecules which are predominant in caucasian phenotypes (HLA-DR1; HLA-DR3; HLA-DR4; HLA-DR7; HLA-DR11; HLA-DR13; HLA-DR15; HLA-DR8). The CD4 T lymphocytes of each donor were isolated from the peripheral blood mononuclear cells (PBMCs), with a degree of purity greater than 98%, by magnetic sorting on a column using magnetic beads coupled to an anti-CD4 antibody, according to a standard procedure defined by the manufacturer (Myltenyi Biotech).

b) Production and Characterization of the DTR-Specific CD4 T Lymphocyte Lines

[0115] 25 overlapping peptides of 15 amino acids covering the entire DTR sequence was synthesized (Intavis Bioanalytical Instruments).

TABLE-US-00002 TABLE II Overlapping peptides covering the DTR sequence (SEQ ID NOs: 18 to 42) Peptide Locali- Amino acid sequence zation 1 378-392 M G Y S P G H K T Q P F L H D 2 385-399 K T Q P F L H D G Y A V S W N 3 391-405 H D G Y A V S W N T V E D S I 4 397-411 S W N T V E D S I I R T G F Q 5 403-417 D S I I R T G F Q G E S G H D 6 409-423 G F Q G E S G H D I K I T A E 7 415-429 G H D I K I T A E N T P L P I 8 421-435 T A E N T P L P I A G V L L P 9 427-441 L P I A G V L L P T I P G K L 10 433-447 L L P T I P G K L D V N K S K 11 439-453 G K L D V N K S K T H I S V N 12 445-459 K S K T H I S V N G R K I R M 13 451-465 S V N G R K I R M R C R A I D 14 457-471 I R M R C R A I D G D V T F C 15 463-477 A I D G D V T F C R P K S P V 16 469-483 T F C R P K S P V Y V G N G V 17 475-489 S P V Y V G N G V H A N L H V 18 482-496 G V H A N L H V A F H R S S S 19 488-502 H V A F H R S S S E K I H S N 20 494-508 S S S E K I H S N E I S S D S 21 500-514 H S N E I S S D S I G V L G Y 22 506-520 S D S I G V L G Y Q K T V D H 23 512-526 L G Y Q K T V D H T K V N S K 24 518-532 V D H T K V N S K L S L F F E 25 521-535 T K V N S K L S L F F E I K S

[0116] The peptides were grouped together in 3 pools:

[0117] Pool 1: peptides 1 to 8

[0118] Pool 2: peptides 9 to 16

[0119] Pool 3: peptides 17 to 25.

[0120] Each of the pools is used in vitro to repeatedly independently sensitize CD4 T lymphocytes from the donors selected for the study. A minimum of 30 coculture wells are produced per pool of peptides. Firstly, the sensitized CD4 T cells capable of recognizing the peptide pool used during the stimulation, called CD4 T lymphocyte lines, are selected by means of an ELISPOT-IFN-.gamma. assay using autologous PBMCs loaded with the peptide pool, as antigen-presenting cells. Secondly, the peptide(s) specifically recognized by the CD4 T lines selected during the first analysis are identified by means of an ELISPOT-IFN-.gamma. assay using autologous PBMCs separately loaded with each of the peptides of the pool, as antigen-presenting cells. The antigen (isolated peptide or peptide pool) recognition specificity is defined by: (1) a ratio between the number of CD4 T lymphocytes producing IFN-.gamma. in response to the antigen (PBMCs+peptides) compared with the background noise (absence of the antigen, i.e. PBMCs alone) which is greater than 2, and (2) a minimum number of 30 spots in the presence of antigen once the background noise has been subtracted.

6) Evaluation of the Antigenicity of the DTR Proteins

[0121] At each step, the incubations were carried out under one or other of the following three conditions: 1 h at 37.degree. C. or 2 h at 20.degree. C. or 16 h at 4.degree. C. The proteins tested (CRM197, catalytic domain, translocation domain, DTR1 and DTR8) were solubilized at the concentration of 1.7.times.10.sup.-8 M in the PBS buffer at pH 7.4 so as to be adsorbed onto 96-well Maxisorp.TM. plates (Nunc). The wells were saturated with a solution of bovine serum albumin (BSA) (Sigma) at 3% in PBS. After 4 washes with a solution of PBS buffer containing 0.05% of Tween 20, the healthy donor sera were incubated in duplicate in the wells after dilution to 1/20.sup.th, 1/200.sup.th or 1/2000.sup.th in a PBS buffer containing 0.2% of BSA and 0.05% of Tween 20. After 4 washes with a solution of PBS buffer containing 0.05% de Tween 20, a goat anti-human IgG antibody conjugated to alkaline phosphatase (Sigma) was incubated in the wells after dilution to 1/200.sup.th in a solution of PBS containing 0.2% of BSA and 0.05% of Tween 20. After 4 washes in a solution of PBS containing 0.05% of Tween 20, the test was visualized by incubation in a 0.1 mM solution of 4-methylumbelliferyl phosphate (Sigma) diluted in the buffer of 50 mM carbonate, 1 mM MgCl.sub.2 at pH 9.8 for 30 min at 20.degree. C. The fluorescence of the wells (emission at 450 nm) was measured in a Victor fluorimeter (Wallac) by excitation at 365 nm.

[0122] Before being tested, the sera were left to stand at 20.degree. C. for a day, and centrifuged at 10 000 g for 10 min, and then 0.003% of thimerosal was added before storage at 4.degree. C. or at -20.degree. C.

EXAMPLE 2

Improvement of the Solubility of the DTR Protein

[0123] The DTR.sub.WT protein expressed in E. coli accumulates in insoluble inclusion bodies. The protein could be obtained, solubilized and purified only in the presence of 0.5% of sarkosyl or of sodium dodecyl sulfate, which are detergents that are incompatible with therapeutic use at these concentrations. The use of other solubilizing molecules was unsuccessful (Tween-80, sucrose, arginine). The use of chaotropic agents such as urea or guanidine chloride for solubilizing the protein, followed by dialysis against various folding buffers, also does not make it possible to obtain a soluble functional protein. The influence of the DTR truncation site relative to the complete diphtheria toxin sequence was also studied. The DTR forms beginning at residue A379, Y380, S381, P382, G383, H384 or K385 were all insoluble in the absence of detergent.

[0124] The strategy used to increase the solubility of the DTR protein consisted in mutating the hydrophobic residues present at the surface of the protein with polar or charged hydrophilic residues. This is because the hydrophobic residues at the surface of a protein are potentially responsible for low solubility and for a tendency to aggregate. The mutations to be introduced were identified on the basis of molecular modeling. The potential effect of the mutations on the structure of the protein is indicated in Table III.

TABLE-US-00003 TABLE III Expected effect of the selected mutations Posi- Muta- tions tions Observation regarding structure and interactions Y380 -- Flexible at the end of the N-ter loop, no stable hydrogen bond Y380E Ionic bond with K385 Y380K Ionic bond with E532 P382 -- In a type II turn P382T Q387 -- In N-ter loop, no stable hydrogen bond Q387E Ionic bond with Y380K P388 -- In N-ter loop, just before a beta strand P388T L390 -- In a beta strand L390N Donor of hydrogen bond for the CO group of the backbone of Y394 L390T Beta-branched residue favored A395 -- In a beta strand A395T Beta-branched residue favored N399 -- In a loop, no stable hydrogen bond N399D Ionic bond with K419 N424 -- In a loop, no stable hydrogen bond N424D Ionic bond with N481K N424E Ionic bond with N481K N424K Ionic bond with E423 or N481E or N481D P426 -- At the end of a loop P426T L427 -- In a beta strand, Van der Waals contacts with Y394 L427K Van der Waals contacts with Y394, donor of hydrogen bond for T425, ionic bond with E423 L427R Van der Waals contacts with Y394, donor of hydrogen bond for T425, ionic bond with E423 P428 -- In a bulge P428T P476 -- In a bulge P476T Y478 -- In a beta strand, Van der Waals contacts with P426, P428, P476 Y478D Y478N N481 -- In a loop, no stable hydrogen bond N481D Ionic bond with N424K N481E Ionic bond with N424K N481K Ionic bond with N424E or N424D V483 -- In a loop V483T

[0125] Three DTR DNA sequence libraries were prepared by synthesis (Table IV). Each library contained sequences mutated on 4 or 5 codons chosen according to the molecular modeling data. Each library corresponded to codons mutated in the same region of the coding sequence. The sequences contained partial degeneracies of the codons to be mutated so as to limit the possible mutations to potentially acceptable hydrophilic residues according to the modeling data (1, 2 or 3 possible mutations per position). The possibility of retaining the wild-type codon was maintained, in the case where the position tested cannot tolerate a mutation (Table IV), except for Y380, the N-terminal position of which, which is relatively not very constrained, was considered to be tolerant.

TABLE-US-00004 TABLE IV Combinations of possible mutations expected for each of the three libraries of mutant sequences generated to increase the solubility of the R domain (R1, R2, R3) Mutated Mutated Possible Library region positions residues diversity R1 Y380 K/E 72 P382 P/T Q387 Q/E/K P388 P/T L390 L/T/N R2 N424 N/K/D/E 48 P426 P/T L427 L/R/K P428 P/T R3 P476 P/T 48 Y478 Y/N/D N481 N/K/D/E V483 V/T *The diversity indicates the number of combinations of mutations possible for each of the libraries.

[0126] After transfection of the DNA libraries, the bacterial colonies obtained, each corresponding to a given mutation combination, were analyzed for their possible capacity to produce a soluble mutant form of the DTR protein. For this, 740 clones derived from the three libraries, representing 85% of the expected diversity (41 different clones for each library), were subcultured in 96-well plates, cultured under protein expression induction conditions, centrifuged, and then lyzed in a lysis solution. After centrifugation, the solubility of the proteins expressed by each clone was evaluated by observation for a possible inclusion body pellet at the bottom of the well and by analysis of the supernatant by polyacrylamide gel electrophoresis under denaturing conditions and with Coomassie blue staining (FIG. 1).

[0127] The analysis of the colonies resulted in selecting the clones where there was an absence of pellet (pellet corresponding to the inclusion bodies) or a pellet of reduced size and also a large amount of protein in the lysis supernatant. Only the R1 library made it possible to generate clones producing soluble DTR (FIG. 1), there being five of said clones. These clones, called 1D3, 1D6, 2C8, 5G5 and 1H3, comprise the nucleotide sequences encoding the proteins SEQ ID NOs: 2 to 6, respectively. All the clones were soluble after purification according to the method described in Example 1. The 1D6 clone, also called G1, which makes it possible to obtain the largest amount of DTR in soluble form after expression at 30.degree. C., carried the mutations: Y380K/Q387E/L390T.

[0128] The modeling approach led to the proposing of two additional mutations, not represented in the R1 library: A395T and N399D. These two mutations were each introduced into the 1D6 clone (or G1 clone) in order to search for an increased solubilizing effect, which was the case for the A395T mutation (FIG. 2).

[0129] In total, the most soluble DTR protein, called DTR1 (SEQ ID NO: 7), carries the mutations: Y380K/Q387E/L390T/A395T.

[0130] This does not rule out the fact that other DTR mutations may further improve its solubility and its production in E. coli.

[0131] The HB-EGF-binding activity of the DTR1 protein was evaluated through its capacity to inhibit the poisoning of Vero cells by diphtheria toxin, and therefore the binding of the toxin to pro-HB-EGF. The results show that DTR1 inhibits the poisoning of Vero cells by diphtheria toxin in a dose-dependent manner (FIG. 3). The inhibitory effect of DTR1 was compared with that of CRM197, of the 1D6 clone and of DTR.sub.WT solubilized in 0.5% of sarkosyl. The Kd values estimated by Schild regression for the three interactions give the following values:

TABLE-US-00005 TABLE V Affinity for HB-EGF Protein Kd (pM) DTR1 ~49 Clone 1D6 ~25 DTR.sub.WT (+0.5% sarkosyl) ~6500 CRM197 ~3100

[0132] Biacore experiments in which HB-EGF was immobilized on the chip of the apparatus and DTR1 was injected into the mobile fraction in order to measure the association and dissociation constants made it possible to confirm the estimated Kd of CRM197 and to show a much higher Kd for DTR1. However, the slowness of the dissociation does not allow an accurate measurement of the dissociation constant of DTR1 in Biacore. In the rest of the study, the affinity of the mutants was estimated by cytotoxicity and S child regression.

EXAMPLE 3

Improvement of the Binding Site of the DTR Protein

[0133] The structure of diphtheria toxin in interaction with HB-EGF (Louie et al., Mol. Cell., 1997, 1, 67-78) makes it possible to analyze the interface between the two proteins. This structural analysis, coupled with in silico molecular modeling experiments, made it possible to select 10 mutations in the DTR binding site in order to increase the affinity of the protein for HB-EGF. These mutations were intended to increase the enthalpy of the interaction by promoting the formation of hydrogen bonds, of salt bridges and/or of Van der Waals contacts between the two proteins. The potential effect of the selected mutations on the structure of the protein is indicated in Table VI.

TABLE-US-00006 TABLE VI Observations of structural nature regarding the residues selected for increasing the affinity of the DTR protein and expected effect of the mutations Positions Mutations Observations of structural nature and potential interactions F389 -- In a beta strand, at the edge of the region of interaction with HB-EGF, para-position close to a water molecule of the structured interface and HB-EGF H139 F389Y Donor or acceptor of hydrogen bond for a water molecule of the structured interface or HB-EGF H139 H391 -- In a beta strand, at the edge of the region of interaction with HB-EGF, donor of hydrogen bond for HB-EGF E141 H391K Ionic bond with HB-EGF E141 G510 -- In a beta strand, at the center of the region of interaction with HB-EGF, next to a cavity between DTR and HB-EGF, opposite the CO group of the backbone of HB-EGF C132 G510A Most conservative change for filling the cavity and increasing Van der Waals contacts G510M Flexible hydrophobic side chain for filling the cavity and increasing Van der Waals contacts G510Q Donor of hydrogen bond for the CO group of the backbone of HB-EGF C132 and/or acceptor of hydrogen H bond for the NH group of the backbone of HB-EGF C134. G510S Small polar side chain for filling the cavity and increasing Van der Waals contacts T521 -- In a type II turn-like structure, at the edge of the region of interaction with HB-EGF T521R Donor of hydrogen bond for the CO group of the backbone of HB-EGF K111 and/or the CO group of the backbone of HB-EGF K113 Q515 -- In a beta strand, at the edge of the region of interaction with HB-EGF, donor of hydrogen bond for the CO group of the backbone of HB-EGF L127 Q515E In an ionic and hydrogen bond network involving K522R, HB-EGF R128, the CO group of the backbone of HB-EGF R128, the CO group of the backbone of HB-EGF L127 K522 -- In a beta strand, at the edge of the region of interaction with HB-EGF, T517 hydrogen bond donor K522R In an ionic and hydrogen bond network involving Q515E, HB-EGF R128, the CO group of the backbone of HB-EGF R128, the CO group of the backbone of HB-EGF L127

[0134] The mutations were introduced into the DTR1 protein by site-directed mutagenesis (Table VII).

TABLE-US-00007 TABLE VII Mutations for improving the HB-EGF-binding affinity of DTR identified by molecular modeling and tested experimentally F389Y G510Q H391K G510S F389Y/H391K T521R G510A Q515E/K522R G510M F389Y/H391K/Q515E/K522R

[0135] The proteins were expressed in E. coli. They were tested for their capacity to inhibit the binding of diphtheria toxin to pro-HB-EGF according to the cytotoxicity test described in Example 1. FIG. 4A shows the capacity of each mutant to possibly inhibit the toxicity of diphtheria toxin on Vero cells. The results show that only the F389Y and G510A mutants significantly inhibit the toxicity of diphtheria toxin. The introduction of these two mutations into DTR1 leads to a cumulative effect (FIG. 4B).

[0136] The most active mutant, corresponding to the DTR1 protein carrying the F389Y and G510A mutations, was called DTR3 (SEQ ID NO: 8). Its HB-EGF-binding affinity was evaluated by the capacity of increasing doses of DTR3 to inhibit the toxic effect of increasing doses of diphtheria toxin on Vero cells as described in Example 2. The Kd estimated by Schild regression from the EC50 values of the inhibition curves is given in Table VIII.

TABLE-US-00008 TABLE VIII Affinity for HB-EGF Protein Kd (pM) DTR3 ~9.5

[0137] This value suggests that DTR3 has an affinity for HB-EGF which is at least 300 times greater than CRM197 and 5 times greater than DTR1.

EXAMPLE 4

Decrease in the Immunogenicity of the DTR Protein by Elimination of the Main CD4 T Epitopes

[0138] The capacity of 25 overlapping peptides covering the entire sequence of DTR.sub.WT to activate specific CD4 T lymphocytes was tested by ELISPOT, in in vitro immunization experiments using CD4 T lymphocytes and dendritic cells purified from the blood of 7 healthy donors, of different age and of different HLA-DRB 1 phenotype.

[0139] The results show that the immune response against the DTR.sub.WT protein is directed predominantly against five epitope regions covering 60% of the protein and against which at least 71% of the donors responded, i.e. 5 donors out of 7 studied (Table IX):

[0140] the L.sub.427-L.sub.441 region (peptide 9) against which specific CD4 T lymphocytes were detected for all of the donors studied with a high magnitude (51 lines among 230 lines screened, i.e. 22%),

[0141] the H.sub.391-Q.sub.411/S.sub.451-D.sub.465/S.sub.475-N.sub.502 regions (peptides 3-4, 13 and 17-18-19 respectively) against which, overall, specific CD4 T cells were detected for 86% of the donors (i.e. 6 donors/7) with a slightly more moderate magnitude than for the region described above (20 to 30 specific CD4 T lymphocyte lines, i.e. 8.5 to 13%), and

[0142] the S.sub.506-H.sub.520 region (peptide 22) against which specific CD4 T cells were detected for 71% of the donors with a magnitude of 8%.

TABLE-US-00009 TABLE IX Results, by peptide and by donor, for the CD4 T lymphocyte lines specific for the DTR protein Donors P668 P661 P659 P663 P664 P667 P658 *Total HLA-DRB1 number of DR3 DR1 DR13 DR4 DR1 DR8 DR3 CD4 T Responder DR13 DR11 DR7 DR11 DR4 DR15 DR7 lines frequency Peptides/Age 52 44 29 62 57 44 30 1 378-392 1 1 14 2 385-399 1 4 2 7 43 3 391-405 1 3 2 1 4 11 71 4 397-411 1 3 1 1 13 8 27 86 5 403-417 1 1 14 6 409-423 1 6 7 29 7 415-429 1 1 14 8 421-435 1 1 2 29 9 427-441 3 3 5 3 13 4 20 51 100 10 433-447 1 2 1 18 22 57 11 439-453 2 2 1 5 43 12 445-459 9 5 3 17 43 13 451-465 9 14 1 2 3 1 30 86 14 457-471 2 1 1 4 43 15 463-477 10 10 14 16 469-483 2 1 3 29 17 475-489 7 2 8 5 1 21 71 18-19 482-502 4 1 5 4 1 3 18 86 20 494-508 2 2 4 29 21 500-514 1 1 4 6 43 22 506-520 1 2 6 4 5 18 71 23 512-526 3 2 5 29 24 518-532 2 1 3 29 25 521-535 1 1 14 Total 27 16 44 23 28 30 40 % 30 13 49 19 31 33 44 Total number of CD4 T lymphocyte lines specific for a peptide among the 230 lines screened

[0143] Since 5 epitope regions were identified, it is possible to envision the mutation of these epitopes in order to reduce their binding to HLA-II molecules. The ProPred server (http://www.imtech.res.in/raghava/propred/) was used to predict, within these five regions of DTR.sub.WT, the sequences capable of binding the 8 HLA class II alleles which are the most common in the population (Table X). The same analysis applied to the mutated sequences of DTR1 shows that the mutations introduced in order to improve the solubility of DTR reduced the immunogenicity of the protein (epitopes predicted for the region 378-403). Indeed, the epitope predicted to bind DRB1.sub.--0301 disappears and one of the epitopes predicted to bind DRB1.sub.--0401 experiences an increase in its threshold, that is to say a decrease in its predicted affinity for the HLA molecule.

TABLE-US-00010 TABLE X Prediction of the sequences capable of binding the 8 HLA class II alleles which are the most common (DRB1_0101, DRB1_0301, DRB1_0401, DRB1_0701, DRB1_1101, DRB1_1301, DRB1_1501, DRB5_0101) within the 5 regions identified as immunogenic by means of a CD4 T lymphocyte activation assay. The peptide sequences correspond to the sequences SEQ ID NOs: 43 to 109. The second column of the table gives the region studied. The first column indicates the mutations proposed for inhibiting the binding of the predicted sequences to the HLA class II molecules. The subsequent columns indicate, for each HLA allele considered, the presence or absence of sequence capable of binding this HLA molecule. Each sequence is preceded by a threshold value reflected in the binding strength: 1/2 (strong binding), 3/4 (medium binding), 5/6 (weak binding). The residues in bold correspond to the mutations proposed for abolishing the binding of the sequences to the HLA allele considered. Variant DRB1_0101 DRB1_0301 DRB1_0401 DRB1_0701 Sequence (378-403) WT MGYSPGHKTQPFLHDGYAVSWNTVED -- 6: FLHDGYAVS 3: FLHDGYAVS -- 5: YAVSWNTVE DTR1 MGKSPGHKTEPFTHDGYTVSWNTVED -- -- 5: FTHDGYTVS -- 5: YTVSWNTVE Sequence (391-411) DTR1 HDGYTVSWNTVEDSIIRTGFQ -- -- 4: VSWNTVEDS 6: WNTVEDSII 5: YTVSWNTVE N399K HDGYTVSWKTVEDSIITGFQ -- -- -- 5: WKTVEDSII V401Q HDGYTVSWNTQEDSIIRTGFQ -- -- 5: YTVSWNTQE -- N399K HDGYTVSWKTQEDSIIRTGFQ -- -- -- -- V401Q Sequence (427-441) DTR1 LPIAGVLLPTIPGKL -- 6: VLLPTIPGK -- 3: LLPTIPGKL L427Q QPIAGVLLPTIPGKL -- 6: VLLPTIPGK -- 3: LLPTIPGKL T436K LPIAGVLLPKIPGKL -- 6: VLLPKIPGK -- -- L427Q QPIAGVLLPKIPGKL -- 6: VLLPKIPGK -- -- T436K Sequence (451-465) DTR1 SVNGRKIRMRCRAID -- 3: IRMRCRAID -- -- I457D SVNGRKDRMRCRAID -- -- -- -- I457E SVNGRKERMRCRAID -- -- -- -- V452T STNGRKIRMRCRAID -- 3: IRMRCRAID -- -- R460T SVNGRKIRMTCRAID -- 3: IRMTCRAID -- -- A463D SVNGRKIRMRCRDID -- 5: IRMRCRDID -- -- V452T STNGRKIRMTCRDID -- 5: IRMTCRDID -- -- R460T A463D Sequence (475-502) DTR1 SPVYVGNGVHANLHVAFHRSSSEKIHSN 3: YVGNGVHAN -- 1: YVGNGVHAN 1: FHRSSSEKI 3: FHRSSSEKI 1: FHRSSSEKI 4: VYVGNGVHA Y478T SPVTVGNGVHANLHVAFHRSSSEKIHSN 3: FHRSSSEKI -- 1: FHRSSSEKI 1: FHRSSSEKI V483D SPVYVGNGDHANLHVAFHRSSSEKIHSN 3: FHRSSSEKI -- 1: FHRSSSEKI 1: FHRSSSEKI 5: YVGNGDHAN V483E SPVYVGNGEHANLHVAFHRSSSEKIHSN 3: FHRSSSEKI -- 1: FHRSSSEKI 1: FHRSSSEKI V483H SPVYVGNGHHANLHVAFHRSSSEKIHSN 3: FHRSSSEKI -- 1: FHRSSSEKI 1: FHRSSSEKI 5: VYVGNGHHA V483Q SPVYVGNGQHANLHVAFHRSSSEKIHSN 3: FHRSSSEKI -- 1: FHRSSSEKI 1: FHRSSSEKI 5: VYVGNGQHA 6: YVGNGQHAN A490G APVYVGNGVHANLHVGFHRSSSEKIHSN 3: YVGNGVHAN -- 1: YVGNGVHAN 1: FHRSSSEKI 3: FHRSSSEKI 1: FHRSSSEKI 4: VYVGNGVHA 6: VGFHRSSSE H492E SPVYVGNGVHANLHVAFERSSSEKIHSN 3: YVGNGVHAN -- 1: YVGNGVHAN 1: FERSSSEKI 4: VYVGNGVHA 2: FERSSSEKI 5: FERSSSEKI S494K SPVYVGNGVHANLHVAFHRKSSEKIHSN 3: YVGNGVHAN -- 1: YVGNGVHAN 2: FHRKSSEKI 4: YVYGNGVHA 5: FHRKSSEKI S496K SPVYVGNGVHANLHVAFHRSSKEKIHSN 3: YVGNGVHAN 4: FHRSSKEKI 1: YVGNGVHAN 1: FHRSSKEKI 4: VYVGNGVHA Y478T SPVTVGNGVHANLHVGFERKSKEKIHSN -- -- -- -- A490G H492E S494K S496K Sequence (506-520) DTR1 SDSIGVLGYQKTVDH -- -- 1: LGYQKTVDH -- T517D SDSIGVLGYQKDVDH -- -- -- -- T517E SDSIGVLGYQKEVDH -- -- -- -- Variant DRB1_1101 DRB1_1301 DRB1_1501 DRB5_0101 Sequence (378-403) WT MGYSPGHKTQPFLHDGYAVSWNTVED -- -- -- -- DTR1 MGKSPGHKTEPFTHDGYTVSWNTVED -- -- -- -- Sequence (391-411) DTR1 HDGYTVSWNTVEDSIIRTGFQ -- -- -- -- N399K HDGYTVSWKTVEDSIITGFQ -- 6: VSWKTVEDS -- -- V401Q HDGYTVSWNTQEDSIIRTGFQ -- -- -- -- N399K HDGYTVSWKTQEDSIIRTGFQ -- -- -- -- V401Q Sequence (427-441) DTR1 LPIAGVLLPTIPGKL 3: LPIAGVLLP 4: LPIAGVLLP -- 2: LLPTIPGKL L427Q QPIAGVLLPTIPGKL -- -- -- 2: LLPTIPGKL T436K LPIAGVLLPKIPGKL 3: LPIAGVLLP 4: LPIAGVLLP -- -- L427Q QPIAGVLLPKIPGKL -- -- -- -- T436K Sequence (451-465) DTR1 SVNGRKIRMRCRAID 3: IRMRCRAID 1: VNGRKIRMR 4: IRMRCRAID -- 1: IRMRCRAID I457D SVNGRKDRMRCRAID -- -- -- -- I457E SVNGRKERMRCRAID -- -- -- -- V452T STNGRKIRMRCRAID 3: IRMRCRAID 1: IRMRCRAID 4: IRMRCRAID -- R460T SVNGRKIRMTCRAID 3: IRMTCRAID 3: VNGRKIRMT 6: IRMTCRAID 3: IRMTCRAID A463D SVNGRKIRMRCRDID -- 1: VNGRKIRMR -- -- 3: IRMRCRDID V452T STNGRKIRMTCRDID -- -- -- -- R460T A463D Sequence (475-502) DTR1 SPVYVGNGVHANLHVAFHRSSSEKIHSN 2: YVGNGVHAN 1: LHVAFHRSS -- -- 5: LHVAFHRSS 4: YVGNGVHAN Y478T SPVTVGNGVHANLHVAFHRSSSEKIHSN 5: LHVAFHRSS 1: LHVAFHRSS -- -- V483D SPVYVGNGDHANLHVAFHRSSSEKIHSN 5: LHVAFHRSS 1: LHVAFHRSS -- -- V483E SPVYVGNGEHANLHVAFHRSSSEKIHSN 5: LHVAFHRSS 1: LHVAFHRSS -- -- V483H SPVYVGNGHHANLHVAFHRSSSEKIHSN 5: VYVGNGHHA 1: LHVAFHRSS 6: VYVGNGHHA -- 5: LHVAFHRSS V483Q SPVYVGNGQHANLHVAFHRSSSEKIHSN 5: YVGNGQHAN 1: LHVAFHRSS -- -- 5: LHVAFHRSS A490G APVYVGNGVHANLHVGFHRSSSEKIHSN 2: YVGNGVHAN 4: YVGNGVHAN -- -- 5: LHVGFHRSS H492E SPVYVGNGVHANLHVAFERSSSEKIHSN 2: YVGNGVHAN 4: YVGNGVHAN -- -- 5: LHVAFERSS S494K SPVYVGNGVHANLHVAFHRKSSEKIHSN 2: YVGNGVHAN 1: LHVAFHRKS -- -- 5: LHVAFHRKS 4: YVGNGVHAN 4: VAFHRKSSE S496K SPVYVGNGVHANLHVAFHRSSKEKIHSN 2: YVGNGVHAN 1: LHVAFHRSS -- -- 5: LHVAFHRSS 4: YVGNGVHAN 5: FHRSSKEKI Y478T SPVTVGNGVHANLHVGFERKSKEKIHSN -- -- -- -- A490G H492E S494K S496K Sequence (506-520) DTR1 SDSIGVLGYQKTVDH 6: LGYQKTVDH 5: LGYQKTVDH -- -- T517D SDSIGVLGYQKDVDH -- -- -- -- T517E SDSIGVLGYQKEVDH -- -- -- --

[0144] The prediction of the anchoring residues of the sequences predicted to bind the HLA class II molecules made it possible to propose a series of mutations indicated in bold in Table X, intended to eliminate potential T epitopes. The mutations were chosen so as to avoid potential destabilization of the protein, which was tested in silico by molecular modeling. Any mutation affecting the HB-EGF-binding site was excluded. The potential effect of the proposed mutations on the structure of the protein is indicated in Table XI.

TABLE-US-00011 TABLE XI Observations of structural nature regarding the residues selected for eliminating CD4 T epitopes of the DTR protein and expected effect of the mutations Positions Mutations Observations of structural nature and potential interactions N399 -- Exposed to the solvent, in a loop, no stable hydrogen bond N399K Ionic bond with D417 and donor of hydrogen bond for the CO group of the backbone of N486 V401 -- Exposed to the solvent, in a type I turn V401Q Acceptor of hydrogen bond for K385 L427 -- Partially buried, in a beta strand, Van der Waals contacts with Y394 L427Q Donor of hydrogen bond for the CO group of the backbone of D392, hydrocarbon-based side chain establishing Van der Waals contacts with Y394 T436 -- Partially buried, in a loop, donor of hydrogen bond for the CO group of the backbone of G466, Van der Waals contacts with V443 T436H Acceptor of hydrogen bond for T469, Van der Waals contacts with V443 T436K Ionic bond with A463D I457 -- Largely buried; in a beta strand; Van der Waals contacts with I450, V452, P473, V477 I457D Acceptor of hydrogen bond for S475, the NH group of the backbone of S475, the NH group of the backbone of K474 I457E Acceptor of hydrogen bond for S475, the NH group of the backbone of S475, the NH group of the backbone of K474, or the NH group of the backbone of V452 V452 -- Partially buried, in a beta strand, Van der Waals contacts by a methyl group of the side chain with I450, I457, V477 V452T Van der Waals contacts by the methyl group of the side chain with I450, I457, V477 A463 -- Exposed to the solvent, in a beta strand A463D Ionic bond with T436K R460 -- Exposed to the solvent, in a beta strand, is part of a basic patch containing other arginines potentially interacting with heparan sulfate groups bonded to the plasma membrane, close to HB-EGF, donor of hydrogen bond for the CO group of the backbone of P473 R460T Beta-branched residue Y478 -- Exposed to the solvent, in a beta strand, Van der Waals contacts with P426, P428, P476 Y478T Beta-branched residue V483 -- Exposed to the solvent, in a loop V483D V483E Acceptor of hydrogen bond for V452T, N453 V483H Donor of hydrogen bond for the CO group of the backbone of Y478 V483Q Acceptor or donor of hydrogen bond for V452T, donor of hydrogen bond for the CO group of the backbone of Y478 A490 -- Partially buried, in a beta strand A490G H492 -- Exposed to the solvent, in a beta strand, acceptor or donor of hydrogen bond for S494 H492E Ionic bond with S494K S494 -- Exposed to the solvent, in a loop, acceptor of hydrogen bond for H492 S494K Ionic bond with H492E S496 -- Exposed to the solvent, in a loop S496K Donor of hydrogend bond for Q411 T517 -- Exposed to the solvent, in a beta strand, acceptor of hydrogen bond for K522 T517D Ionic bond with K522 T517E Ionic bond with K522

[0145] The mutations indicated in Table XII were introduced individually, or sometimes in combination, into the sequence of the DTR3 protein, according to the modeling data, then gradually accumulated when they altered neither the production of the recombinant protein nor its biological activity. The biological activity of the mutants was tested by inhibition of the toxicity of diphtheria toxin on Vero cells.

TABLE-US-00012 TABLE XII Mutations introduced into DTR3 in order to eliminate CD4 T epitopes N399K A463S H492E V401Q A463E S494K L427Q A463D S496K L427N A463G H492E/S494K L427S T436K/A463D H492E/S496K T436K V452T/R460T/A463D S494K/S496K T436H Y478T H492E/S494K/S496K V452T V483D E497D I457D V483E T436H/E497D I457E V483H T517D R460T V483Q T517E A463T A490G * In bold, the mutations which do not significantly affect the expression and the activity of the protein, which were therefore retained.

[0146] These experiments made it possible to successively retain the following mutations: N399K, V452T, T517E, V483Q, H492E, S494K, T436H and E497D.

[0147] The protein derived from DTR3 and accumulating the N399K, V452T, T517E, V483Q, H492E and S494K mutations is called DTR8 (SEQ ID NO: 9); it has an MW of 17458 Da. These mutations made it possible to eliminate 10 of the 26 CD4 T epitopes identified in DTR.sub.WT (Table XIII).

TABLE-US-00013 TABLE XIII Comparison of the number and of the strength of the binding to HLA II of the CD4 T epitopes between DTR.sub.WT and DTR8 Strength of Threshold value CD4 T epitopes binding to reflecting the binding Total DTR HLA II strength 391-411 427-441 451-465 475-502 506-520 number DTR.sub.WT Strong 1 0 0 2 4 1 7 2 0 1 0 1 0 2 Medium 3 0 2 2 2 0 6 4 1 1 1 2 0 5 Weak 5 1 0 0 1 1 3 6 1 1 0 0 1 3 Total 3 5 5 10 3 26 DTR8 Strong 1 0 0 1 0 0 1 2 0 1 0 0 0 1 Medium 3 0 2 2 1 0 5 4 0 1 1 0 0 2 Weak 5 1 0 0 3 0 4 6 1 1 0 1 0 3 Total 2 5 4 5 0 16

[0148] Among these 10 epitopes are 7 of the 9 epitopes predicted as being immunodominant epitopes of DTR.sub.WT. The capacity of the resulting protein, DTR8, to induce a CD4-type immune response, i.e. producing antibodies, is thus considerably reduced compared with that of DTR.sub.WT in its wild-type form.

[0149] DTR8 contains six more mutations than DTR3. Since any mutation or mutation combination is capable of impairing the function of a protein, it is necessary to evaluate the effect of these additional mutations on the DTR binding affinity for HB-EGF. The HB-EGF-binding activity of the DTR8 protein was evaluated through its capacity to inhibit the poisoning of Vero cells by diphtheria toxin, and therefore the binding of the toxin to pro-HB-EGF. The results (FIG. 5) show that DTR8 inhibits the poisoning of Vero cells by diphtheria toxin in a dose-dependent manner. The inhibitory effect of DTR8 was compared with that of CRM197, DTR.sub.WT solubilized in 0.5% of sarkosyl, DTR3 and DTR1 (FIG. 3). The Kd values estimated by Schild regression for the four interactions give the following values (Table XIV).

TABLE-US-00014 TABLE XIV Affinity of the proteins for HB-EGF Protein Kd (pM) DTR8 2.2 DTR3 9.5 DTR1 49 DTR.sub.WT (+0.5% sarkosyl) 6500 CRM197 3100

[0150] Notably and unexpectedly, the addition of the six mutations intended to reduce the immunogenicity of DTR contributed to increasing its affinity for HB-EGF. Overall, DTR8 has approximately 1400 times more affinity for HB-EGF than CRM197.

[0151] The HB-EGF-binding activity of the DTR8 protein was also evaluated through its capacity to inhibit the binding of HB-EGF to the EGFR in a Ba/F3 cell line transfected with the EGFR gene and dependent on HB-EGF for its growth. The results (FIG. 6) show that DTR8 inhibits the proliferation of the HB-EGF-dependent cells in a dose-dependent manner. The inhibitory effect of DTR8 was compared with that of CRM197 (FIG. 6). The results show that at least 300 times more CRM197 is necessary in order to obtain an equivalent inhibitory effect. Consequently, DTR8 is at least 300 times more effective than CRM197 in terms of binding to HB-EGF in solution.

[0152] In conclusion, these results show that the DTR8 protein is capable of binding to pro-HB-EGF molecules at the surface of cells (FIG. 5) and to HB-EGF in solution (FIG. 6) and of blocking their biological activity. The estimation of the Kd values for the interactions suggests that DTR8 is 1400 times more powerful than CRM197 in terms of binding to HB-EGF.

EXAMPLE 5

Evaluation of the Antigenicity of the DTR1 and DTR8 Proteins

[0153] The western population is vaccinated against diphtheria toxin. Individuals who may benefit from treatment with a therapeutic protein of DTR8 type could therefore have antibodies capable of reacting against said protein. The capacity of the sera of 20 healthy donors to recognize diphtheria toxin (in its mutated form CRM197), and its various domains (catalytic (C), translocation (T) and DTR (in the soluble mutated form DTR1)) was compared with the DTR8 protein, by ELISA (FIG. 7). An antibody titer 20 corresponds to the background noise of the assay and therefore to an absence of recognition. A titer threshold at 30 was arbitrarily set in order to distinguish the sera exhibiting a weak or strong response.

[0154] The results (FIG. 7) show that 16/20 donors have antibodies against diphtheria toxin. 14/20 donors exhibit a medium to strong antibody response. However, the majority of the antibodies present in each serum considered individually are directed against the C domain of the toxin (FIG. 7). Indeed, 12 sera exhibit a response above the threshold (medium to strong response) and 4 sera exhibit a response below the threshold (weak response). If the reactivity of the sera against the soluble form of the R domain (DTR1) is considered, 15/20 sera recognize the R domain. However, only 7 sera exhibit a response above the threshold (medium to strong response). Notably, the DTR8 protein is considerably less well recognized by the sera of the donors than DTR1. Indeed, only 3/20 sera exhibit medium to strong reactivity against DTR8 and 2 sera exhibit weak reactivity (FIG. 7).

[0155] In conclusion, these results show that the antigenicity of the DTR8 protein is weak and considerably reduced compared with that of CRM197. This antigenicity is also reduced in comparison with that of the DTR1 protein, corresponding to the soluble form of DTR carrying the lowest number of mutations. In other words, the mutations introduced into DTR in order to increase its affinity for HB-EGF and to reduce its immunogenicity contribute to considerably reducing its antigenicity.

Sequence CWU 1

1

1091535PRTCorynebacterium diphtheriae 1Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 1 5 10 15 Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 20 25 30 Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 35 40 45 Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 50 55 60 Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 65 70 75 80 Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 85 90 95 Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 100 105 110 Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 115 120 125 Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 130 135 140 Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 145 150 155 160 Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 165 170 175 Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 180 185 190 Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 195 200 205 Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 210 215 220 Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 225 230 235 240 Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 245 250 255 His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val 260 265 270 Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 275 280 285 Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 290 295 300 Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 305 310 315 320 Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 325 330 335 Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 340 345 350 Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 355 360 365 Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 370 375 380 Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 385 390 395 400 Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 405 410 415 Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 420 425 430 Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 435 440 445 His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 450 455 460 Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 465 470 475 480 Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 485 490 495 Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 500 505 510 Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser 515 520 525 Leu Phe Phe Glu Ile Lys Ser 530 535 2158PRTartificial sequencesynthetic protein (DTR variant Y380K/L390T encoded by clone 1D3) 2Met Gly Lys Ser Pro Gly His Lys Thr Gln Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 3158PRTartificial sequencesynthetic protein (DTR variant Y380K/Q387E/ L390T encoded by clone 1D6) 3Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 4158PRTartificial sequencesynthetic protein (DTR variant Y380K/Q387E/ P388T/L390T encoded by clone 2C8) 4Met Gly Lys Ser Pro Gly His Lys Thr Glu Thr Phe Thr His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 5158PRTartificial sequencesynthetic protein (DTR variant Y380K/P382T/ Q387E/L390T encoded by clone 2G5) 5Met Gly Lys Ser Thr Gly His Lys Thr Glu Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 6158PRTartificial sequencesynthetic protein (DTR variant Y380E/P382T/ Q387K/L390T encoded by clone 1H3) 6Met Gly Glu Ser Thr Gly His Lys Thr Lys Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 7158PRTartificial sequencesynthetic protein (DTR variant Y380K/Q387E/ L390T/A395T named as DTR1) 7Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 8158PRTartificial sequencesynthetic protein (DTR variant Y380K/Q387E/F389Y/L390T/A395T/G510A named as DTR3) 8Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Tyr Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Ala Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 9158PRTartificial sequencesynthetic protein (DTRvariant Y380K/Q387E/F389Y/L390T/A395T/N399K/V452T/V483Q/H492E/S494K/G510A /T517E named as DTR8) 9Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Tyr Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Lys Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Thr Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Gln His Ala Asn Leu His Val 100 105 110 Ala Phe Glu Arg Lys Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Ala Val Leu Gly Tyr Gln Lys Glu Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 10477DNAartificial sequencesynthetic polynucleotide (optimized coding sequence for DTR1) 10atg ggc aaa agc ccg ggt cat aaa acc gag ccg ttt acc cat gat ggc 48Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Phe Thr His Asp Gly 1 5 10 15 tat acc gtg agc tgg aac acc gtg gaa gat agc att att cgt acc ggc 96Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 ttt cag ggc gaa agc ggc cat gat att aaa att acc gcg gaa aac acc 144Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 ccg ctg ccg att gcg ggt gtt ctg ctg ccg acc att ccg ggc aaa ctg 192Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 gat gtg aac aaa agc aaa acc cat att agc gtg aac ggc cgt aaa att 240Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 cgt atg cgt tgc cgt gcg att gat ggt gat gtg acc ttt tgc cgt ccg 288Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 aaa agc ccg gtg tat gtg ggc aac ggc gtg cat gcg aac ctg cat gtg 336Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 gcg ttt cat cgt agc agc agc gaa aaa atc cat agc aac gaa att agc 384Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 agc gat agc att ggc gtg ctg ggc tat cag aaa acc gtg gat cat acc 432Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 aaa gtg aac tct aaa ctg agc ctg ttc ttc gaa atc aaa agc tga 477Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 11158PRTartificial sequenceSynthetic Construct 11Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70

75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 12477DNAartificial sequencesynthetic polynucleotide (optimized coding sequence for DTR3) 12atg ggc aaa agc ccg ggt cat aaa acc gag ccg tat acc cat gat ggc 48Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Tyr Thr His Asp Gly 1 5 10 15 tat acc gtg agc tgg aac acc gtg gaa gat agc att att cgt acc ggc 96Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 ttt cag ggc gaa agc ggc cat gat att aaa att acc gcg gaa aac acc 144Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 ccg ctg ccg att gcg ggt gtt ctg ctg ccg acc att ccg ggc aaa ctg 192Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 gat gtg aac aaa agc aaa acc cat att agc gtg aac ggc cgt aaa att 240Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 cgt atg cgt tgc cgt gcg att gat ggt gat gtg acc ttt tgc cgt ccg 288Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 aaa agc ccg gtg tat gtg ggc aac ggc gtg cat gcg aac ctg cat gtg 336Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 gcg ttt cat cgt agc agc agc gaa aaa atc cat agc aac gaa att agc 384Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 agc gat agc att gcg gtg ctg ggc tat cag aaa acc gtg gat cat acc 432Ser Asp Ser Ile Ala Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 aaa gtg aac tct aaa ctg agc ctg ttc ttc gaa atc aaa agc tga 477Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 13158PRTartificial sequenceSynthetic Construct 13Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Tyr Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Ala Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 14477DNAartificial sequencesynthetic polynucleotide (optimized coding sequence for DTR8) 14atg ggc aaa agc ccg ggt cat aaa acc gag ccg tat acc cat gat ggc 48Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Tyr Thr His Asp Gly 1 5 10 15 tat acc gtg agc tgg aaa acc gtg gaa gat agc att att cgt acc ggc 96Tyr Thr Val Ser Trp Lys Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 ttt cag ggc gaa agc ggc cat gat att aaa att acc gcg gaa aac acc 144Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 ccg ctg ccg att gcg ggt gtt ctg ctg ccg acc att ccg ggc aaa ctg 192Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 gat gtg aac aaa agc aaa acc cat att agc acg aac ggc cgt aaa att 240Asp Val Asn Lys Ser Lys Thr His Ile Ser Thr Asn Gly Arg Lys Ile 65 70 75 80 cgt atg cgt tgc cgt gcg att gat ggt gat gtg acc ttt tgc cgt ccg 288Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 aaa agc ccg gtg tat gtg ggc aac ggc cag cat gcg aac ctg cat gtg 336Lys Ser Pro Val Tyr Val Gly Asn Gly Gln His Ala Asn Leu His Val 100 105 110 gcg ttt gaa cgt aaa agc agc gaa aaa atc cat agc aac gaa att agc 384Ala Phe Glu Arg Lys Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 agc gat agc att gcg gtg ctg ggc tat cag aaa gaa gtg gat cat acc 432Ser Asp Ser Ile Ala Val Leu Gly Tyr Gln Lys Glu Val Asp His Thr 130 135 140 aaa gtg aac tct aaa ctg agc ctg ttc ttc gaa atc aaa agc tga 477Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 15158PRTartificial sequenceSynthetic Construct 15Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Tyr Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Lys Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Thr Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Gln His Ala Asn Leu His Val 100 105 110 Ala Phe Glu Arg Lys Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Ala Val Leu Gly Tyr Gln Lys Glu Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 16477DNAartificial sequencesynthetic polynucleotide (optimized coding sequence for DTRwt) 16atg ggc tac agc ccg ggt cat aaa acc cag ccg ttt ctg cat gat ggc 48Met Gly Tyr Ser Pro Gly His Lys Thr Gln Pro Phe Leu His Asp Gly 1 5 10 15 tat gcg gtg agc tgg aac acc gtg gaa gat agc att att cgt acc ggc 96Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 ttt cag ggc gaa agc ggc cat gat att aaa att acc gcg gaa aac acc 144Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 ccg ctg ccg att gcg ggt gtt ctg ctg ccg acc att ccg ggc aaa ctg 192Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 gat gtg aac aaa agc aaa acc cat att agc gtg aac ggc cgt aaa att 240Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 cgt atg cgt tgc cgt gcg att gat ggt gat gtg acc ttt tgc cgt ccg 288Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 aaa agc ccg gtg tat gtg ggc aac ggc gtg cat gcg aac ctg cat gtg 336Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 gcg ttt cat cgt agc agc agc gaa aaa atc cat agc aac gaa att agc 384Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 agc gat agc att ggc gtg ctg ggc tat cag aaa acc gtg gat cat acc 432Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 aaa gtg aac tct aaa ctg agc ctg ttc ttc gaa atc aaa agc tga 477Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 17158PRTartificial sequenceSynthetic Construct 17Met Gly Tyr Ser Pro Gly His Lys Thr Gln Pro Phe Leu His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly 20 25 30 Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr 35 40 45 Pro Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 50 55 60 Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile 65 70 75 80 Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro 85 90 95 Lys Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 100 105 110 Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser 115 120 125 Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr 130 135 140 Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 145 150 155 1815PRTartificial sequencesynthetic peptide (peptide 1 DTR 378-392) 18Met Gly Tyr Ser Pro Gly His Lys Thr Gln Pro Phe Leu His Asp 1 5 10 15 1915PRTartificial sequencesynthetic peptide (peptide 2 DTR 385-399) 19Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn 1 5 10 15 2015PRTartificial sequencesynthetic peptide (peptide 3 DTR 391-405) 20His Asp Gly Tyr Ala Val Ser Trp Asn Thr Val Glu Asp Ser Ile 1 5 10 15 2115PRTartificial sequencesynthetic peptide (peptide 4 DTR 397-411) 21Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln 1 5 10 15 2215PRTartificial sequencesynthetic peptide (peptide 5 DTR 403-417) 22Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His Asp 1 5 10 15 2315PRTartificial sequencesynthetic peptide (peptide 6 DTR 409-423) 23Gly Phe Gln Gly Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu 1 5 10 15 2415PRTartificial sequencesynthetic peptide (peptide 7 DTR 415-429) 24Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile 1 5 10 15 2515PRTartificial sequencesynthetic peptide (peptide 8 DTR 421-435) 25Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val Leu Leu Pro 1 5 10 15 2615PRTartificial sequencesynthetic peptide (peptide 9 DTR 427-441) 26Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 1 5 10 15 2715PRTartificial sequencesynthetic peptide (peptide 10 DTR 433-447) 27Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys 1 5 10 15 2815PRTartificial sequencesynthetic peptide (peptide 11 DTR 439-453) 28Gly Lys Leu Asp Val Asn Lys Ser Lys Thr His Ile Ser Val Asn 1 5 10 15 2915PRTartificial sequencesynthetic peptide (peptide 12 DTR 445-459) 29Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile Arg Met 1 5 10 15 3015PRTartificial sequencesynthetic peptide (peptide 13 DTR 451-465) 30Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile Asp 1 5 10 15 3115PRTartificial sequencesynthetic peptide (peptide 14 DTR 457-471) 31Ile Arg Met Arg Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys 1 5 10 15 3215PRTartificial sequencesynthetic peptide (peptide 15 DTR 463-477) 32Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val 1 5 10 15 3315PRTartificial sequencesynthetic peptide (peptide 16 DTR 469-483) 33Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly Asn Gly Val 1 5 10 15 3415PRTartificial sequencesynthetic peptide (peptide 17 DTR 475-489) 34Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val 1 5 10 15 3515PRTartificial sequencesynthetic peptide (peptide 18 DTR 482-496) 35Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 1 5 10 15 3615PRTartificial sequencesynthetic peptide (peptide 19 DTR 488-502) 36His Val Ala Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 1 5 10 15 3715PRTartificial sequencesynthetic peptide (peptide 20 DTR 494-508) 37Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser 1 5 10 15 3815PRTartificial sequencesynthetic peptide (peptide 21 DTR 500-514) 38His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu Gly Tyr 1 5 10 15 3915PRTartificial sequencesynthetic peptide (peptide 22 DTR 506-520) 39Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His 1 5 10 15 4015PRTartificial sequencesynthetic peptide (peptide 23 DTR 512-526) 40Leu Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys 1 5 10 15 4115PRTartificial sequencesynthetic peptide (peptide 24 DTR 518-532) 41Val Asp His Thr Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu 1 5 10 15 4215PRTartificial sequencesynthetic peptide (peptide 25 DTR 521-535) 42Thr Lys Val Asn Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser 1 5 10 15 4326PRTartificial sequencesynthetic peptide (DTRwt 378-403) 43Met Gly Tyr Ser Pro Gly His Lys Thr Gln Pro Phe Leu His Asp Gly 1 5 10 15 Tyr Ala Val Ser Trp Asn Thr Val Glu Asp 20 25 4426PRTartificial sequencesynthetic peptide (DTR1 378-403) 44Met Gly Lys Ser Pro Gly His Lys Thr Glu Pro Phe Thr His Asp Gly 1 5 10 15 Tyr Thr Val Ser Trp Asn Thr Val Glu Asp 20 25 459PRTartificial sequencesynthetic peptide (DTRwt 389-397) 45Phe Leu His Asp Gly Tyr Ala Val Ser 1 5 469PRTartificial sequencesynthetic peptide (DTRwt 394-402) 46Tyr Ala Val Ser Trp Asn Thr Val Glu 1 5 479PRTartificial sequencesynthetic peptide (DTR1 389-397) 47Phe Thr His Asp Gly Tyr Thr Val Ser 1 5 489PRTartificial sequencesynthetic peptide (DTR1 394-402) 48Tyr Thr Val Ser Trp Asn Thr Val Glu 1 5 4921PRTartificial sequencesynthetic peptide (DTR1 391-411) 49His Asp Gly Tyr Thr Val Ser Trp Asn Thr Val Glu Asp Ser Ile Ile 1 5 10 15 Arg Thr Gly Phe Gln 20 5021PRTartificial sequencesynthetic peptide (N399K variant 391-411) 50His Asp Gly Tyr Thr Val Ser Trp Lys Thr Val Glu Asp Ser Ile Ile 1 5 10 15 Arg Thr Gly Phe Gln 20 5121PRTartificial sequencesynthetic peptide (V401Q variant 391-411) 51His Asp Gly Tyr Thr Val Ser Trp Asn Thr Gln Glu Asp Ser Ile Ile 1 5 10 15 Arg Thr Gly Phe Gln 20 5221PRTartificial sequencesynthetic peptide (N399K/V401Q variant 391-411) 52His Asp Gly Tyr Thr Val Ser Trp Lys Thr Gln Glu Asp Ser Ile Ile 1 5 10 15 Arg Thr Gly Phe Gln 20

539PRTartificial sequencesynthetic peptide (DTR1 396-404) 53Val Ser Trp Asn Thr Val Glu Asp Ser 1 5 549PRTartificial sequencesynthetic peptide (DTR1 394-402) 54Tyr Thr Val Ser Trp Asn Thr Val Glu 1 5 559PRTartificial sequencesynthetic peptide (DTR1 398-406) 55Trp Asn Thr Val Glu Asp Ser Ile Ile 1 5 569PRTartificial sequencesynthetic peptide (N399K variant 398-406) 56Trp Lys Thr Val Glu Asp Ser Ile Ile 1 5 579PRTartificial sequencesynthetic peptide (N399K variant 396-404) 57Val Ser Trp Lys Thr Val Glu Asp Ser 1 5 589PRTartificial sequencesynthetic peptide (V401Q variant 394-402) 58Tyr Thr Val Ser Trp Asn Thr Gln Glu 1 5 5915PRTartificial sequencesynthetic peptide (DTR1 427-441) 59Leu Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 1 5 10 15 6015PRTartificial sequencesynthetic peptide (L427Q variant 427-441) 60Gln Pro Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu 1 5 10 15 6115PRTartificial sequencesynthetic peptide (T436K variant 427-441) 61Leu Pro Ile Ala Gly Val Leu Leu Pro Lys Ile Pro Gly Lys Leu 1 5 10 15 6215PRTartificial sequencesynthetic peptide (L427Q/T436K variant 427-441) 62Gln Pro Ile Ala Gly Val Leu Leu Pro Lys Ile Pro Gly Lys Leu 1 5 10 15 639PRTartificial sequencesynthetic peptide (DTR1 432-440) 63Val Leu Leu Pro Thr Ile Pro Gly Lys 1 5 649PRTartificial sequencesynthetic peptide (T436K variant 432-440) 64Val Leu Leu Pro Lys Ile Pro Gly Lys 1 5 659PRTartificial sequencesynthetic peptide (DTR1 433-441) 65Leu Leu Pro Thr Ile Pro Gly Lys Leu 1 5 669PRTartificial sequencesynthetic peptide (DTR1 427-435) 66Leu Pro Ile Ala Gly Val Leu Leu Pro 1 5 6715PRTartificial sequencesynthetic peptide (DTR1 451-465) 67Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile Asp 1 5 10 15 6815PRTartificial sequencesynthetic peptide (I457D variant 451-465) 68Ser Val Asn Gly Arg Lys Asp Arg Met Arg Cys Arg Ala Ile Asp 1 5 10 15 6915PRTartificial sequencesynthetic peptide (I457E variant 451-465) 69Ser Val Asn Gly Arg Lys Glu Arg Met Arg Cys Arg Ala Ile Asp 1 5 10 15 7015PRTartificial sequencesynthetic peptide (V452T variant 451-465) 70Ser Thr Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile Asp 1 5 10 15 7115PRTartificial sequencesynthetic peptide (R460T variant 451-465) 71Ser Val Asn Gly Arg Lys Ile Arg Met Thr Cys Arg Ala Ile Asp 1 5 10 15 7215PRTartificial sequencesynthetic peptide (A463D variant 451-465) 72Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Asp Ile Asp 1 5 10 15 7315PRTartificial sequencesynthetic peptide (V452T/R460T/A463D variant 451-465) 73Ser Thr Asn Gly Arg Lys Ile Arg Met Thr Cys Arg Asp Ile Asp 1 5 10 15 749PRTartificial sequencesynthetic peptide (DTR1 457-465) 74Ile Arg Met Arg Cys Arg Ala Ile Asp 1 5 759PRTartificial sequencesynthetic peptide (R460T variant 457-465) 75Ile Arg Met Thr Cys Arg Ala Ile Asp 1 5 769PRTartificial sequencesynthetic peptide (A463D variant 457-465) 76Ile Arg Met Arg Cys Arg Asp Ile Asp 1 5 779PRTartificial sequencesynthetic peptide (V452T/R460T/A463D variant 451-465) 77Ile Arg Met Thr Cys Arg Asp Ile Asp 1 5 789PRTartificial sequencesynthetic peptide (DTR1 452-460) 78Val Asn Gly Arg Lys Ile Arg Met Arg 1 5 799PRTartificial sequencesynthetic peptide (R460T variant 452-460) 79Val Asn Gly Arg Lys Ile Arg Met Thr 1 5 8028PRTartificial sequencesynthetic peptide (DTR1 475-502) 80Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8128PRTartificial sequencesynthetic peptide (Y478T variant 475-502) 81Ser Pro Val Thr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8228PRTartificial sequencesynthetic peptide (V483D variant 475-502) 82Ser Pro Val Tyr Val Gly Asn Gly Asp His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8328PRTartificial sequencesynthetic peptide (V483E variant 475-502) 83Ser Pro Val Tyr Val Gly Asn Gly Glu His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8428PRTartificial sequencesynthetic peptide (V483H variant 475-502) 84Ser Pro Val Tyr Val Gly Asn Gly His His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8528PRTartificial sequencesynthetic peptide (V483Q variant 475-502) 85Ser Pro Val Tyr Val Gly Asn Gly Gln His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8628PRTartificial sequencesynthetic peptide (A490G variant 475-502) 86Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Gly 1 5 10 15 Phe His Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8728PRTartificial sequencesynthetic peptide (H492E variant 475-502) 87Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala 1 5 10 15 Phe Glu Arg Ser Ser Ser Glu Lys Ile His Ser Asn 20 25 8828PRTartificial sequencesynthetic peptide (S494K variant 475-502) 88Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Lys Ser Ser Glu Lys Ile His Ser Asn 20 25 8928PRTartificial sequencesynthetic peptide (S496K variant 475-502) 89Ser Pro Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala 1 5 10 15 Phe His Arg Ser Ser Lys Glu Lys Ile His Ser Asn 20 25 9028PRTartificial sequencesynthetic peptide (Y478T/A490G/H492E/S494K/ S496K variant 475-502) 90Ser Pro Val Thr Val Gly Asn Gly Val His Ala Asn Leu His Val Gly 1 5 10 15 Phe Glu Arg Lys Ser Lys Glu Lys Ile His Ser Asn 20 25 919PRTartificial sequencesynthetic peptide (DTR1 478-486) 91Tyr Val Gly Asn Gly Val His Ala Asn 1 5 929PRTartificial sequencesynthetic peptide (DTR1 491-499) 92Phe His Arg Ser Ser Ser Glu Lys Ile 1 5 939PRTartificial sequencesynthetic peptide (DTR1 477-485) 93Val Tyr Val Gly Asn Gly Val His Ala 1 5 949PRTartificial sequencesynthetic peptide (DTR1 487-495) 94Leu His Val Ala Phe His Arg Ser Ser 1 5 959PRTartificial sequencesynthetic peptide (V483D variant 478-486) 95Tyr Val Gly Asn Gly Asp His Ala Asn 1 5 969PRTartificial sequencesynthetic peptide (V483H variant 477-485) 96Val Tyr Val Gly Asn Gly His His Ala 1 5 979PRTartificial sequencesynthetic peptide (V483Q variant 477-485) 97Val Tyr Val Gly Asn Gly Gln His Ala 1 5 989PRTartificial sequencesynthetic peptide (V483Q variant 478-486) 98Tyr Val Gly Asn Gly Gln His Ala Asn 1 5 999PRTartificial sequencesynthetic peptide (A490G variant 487-495) 99Leu His Val Gly Phe His Arg Ser Ser 1 5 1008PRTartificial sequencesynthetic peptide (H492E variant 491-499) 100Phe Glu Arg Ser Ser Ser Glu Lys 1 5 1019PRTartificial sequencesynthetic peptide (H492E variant 487-495) 101Leu His Val Ala Phe Glu Arg Ser Ser 1 5 1029PRTartificial sequencesynthetic peptide (S494K variant 491-499) 102Phe His Arg Lys Ser Ser Glu Lys Ile 1 5 1039PRTartificial sequencesynthetic peptide (S494K variant 487-495) 103Leu His Val Ala Phe His Arg Lys Ser 1 5 1049PRTartificial sequencesynthetic peptide (S494K variant 489-497) 104Val Ala Phe His Arg Lys Ser Ser Glu 1 5 1059PRTartificial sequencesynthetic peptide (S496K variant 491-499) 105Phe His Arg Ser Ser Lys Glu Lys Ile 1 5 10615PRTartificial sequencesynthetic peptide (DTR1 506-520) 106Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His 1 5 10 15 10715PRTartificial sequencesynthetic peptide (T517D variant 506-520) 107Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Asp Val Asp His 1 5 10 15 10815PRTartificial sequencesynthetic peptide (T517E variant 506-520) 108Ser Asp Ser Ile Gly Val Leu Gly Tyr Gln Lys Glu Val Asp His 1 5 10 15 1099PRTartificial sequencesynthetic peptide (DTR1 512-520) 109Leu Gly Tyr Gln Lys Thr Val Asp His 1 5

Claims

1. A recombinant protein comprising an amino acid sequence having at least 70% similarity with residues 380 to 535 of the amino acid sequence SEQ ID NO: 1 which correspond to a R domain of diphtheria toxin, wherein the amino acid sequence comprises a substitution of at least one residue selected from the group consisting of Y380, P382, Q387, P388 and L390 of the R domain with another amino acid selected from the group consisting of: S, T, N, C, Y, Q, R, K, H, D and E, the amino acid sequence is devoid, at the N- or C-terminal end of the R domain, of the sequence of the T domain or of the C domain and of the T domain of diphtheria toxin, and of the sequence of a protein or of a protein domain capable of improving stability or purification of the R domain, and the recombinant protein is a ligand inhibiting HB-EGF.

2. The protein as claimed in claim 1, comprising at least two substitutions selected from the group consisting of Y380K, Y380E, P382T, Q387E, Q387K, P388T, L390T and L390N.

3. The protein as claimed in claim 2, comprising at least the substitutions Y380K and L390T or Y380K and Q387E.

4. The protein as claimed in claim 3, comprising at least the substitutions Y380K, Q387E and L390T.

5. The protein as claimed in claim 1, further comprising a substitution A395T.

6. The protein as claimed in claim 1, further comprising at least one substitution of F389Y and G510A.

7. The protein as claimed in claim 1, further comprising at least one substitution selected from the group consisting of: N399K, V452T, T517E, V483Q, H492E, S494K, T436H and E497D.

8. The protein as claimed in claim 7, comprising substitutions N399K, V452T, T517E, V483Q, H492E and S494K.

9. The protein as claimed in claim 1, comprising one of amino acid sequences SEQ ID NOs: 2 to 9.

10. The protein as claimed in claim 1, which is labeled with a detectable tracer.

11. The protein as claimed in claim 10, wherein the detectable tracer is a radioactive isotope or a fluorophore.

12. An isolated polynucleotide encoding the recombinant protein as claimed in claim 1.

13. The polynucleotide as claimed in claim 12, comprising one of nucleotide sequences SEQ ID NOs: 10, 12 or 14.

14. A recombinant cloning and/or expression vector comprising the polynucleotide as claimed in claim 12.

15. A cell modified with the recombinant cloning and/or expression vector as claimed in claim 14.

16. A pharmaceutical composition, comprising at least one recombinant protein as claimed in claim 1, and a pharmaceutically acceptable vehicle.

17. A method for treating a disease associated with activation of HB-EGF/EGFR pathway in a subject in need thereof, the method comprising: administering the recombinant protein as claimed in claim 1 to the subject, wherein the disease is selected from the group consisting of: rapidly progressive glomerulonephritis, cancers, vasospasm associated with cerebral contusions, cardiac hypertrophy, smooth muscle cell hyperplasia, pulmonary hypertension, diabetic retinopathies and arterial restenosis.

18. A method for an in vitro diagnosis of a disease associated with activation of HB-EGF/EGFR pathway, the method comprising: obtaining a biological sample from a subject, contacting the protein as claimed in claim 10 with the biological sample, thereby forming a complex between the protein and HB-EGF or pro-HB-EGF, detecting the presence of the complex, and diagnosing the subject as having the disease where the presence of the complex is detected, wherein the disease is selected from the group consisting of: rapidly progressive glomerulonephritis, cancers, vasospasm associated with cerebral contusions, cardiac hypertrophy, smooth muscle cell hyperplasia, pulmonary hypertension, diabetic retinopathies and arterial restenosis.

19. A pharmaceutical composition, comprising the recombinant cloning and/or expression vector as claimed in claim 14, and a pharmaceutically acceptable vehicle.

20. A method for treating a disease associated with activation of HB-EGF/EGFR pathway, the method comprising: administering the recombinant cloning and/or expression vector as claimed in claim 14 to a recipient in need thereof, wherein the disease is selected from the group consisting of: rapidly progressive glomerulonephritis, cancers, vasospasm associated with cerebral contusions, cardiac hypertrophy, smooth muscle cell hyperplasia, pulmonary hypertension, diabetic retinopathies and arterial restenosis.