U.S. patent application number 14/318425 was filed with the patent office on 2014-11-20 for conjugation of biomolecules using diels-alder cycloaddition.
This patent application is currently assigned to The United States of America, as represented by the Secretary, Department of Health and Human Serv. The applicant listed for this patent is The United States of America, as represented by the Secretary, Department of Health and Human Serv, The United States of America, as represented by the Secretary, Department of Health and Human Serv. Invention is credited to Vince Pozsgay.
Application Number | 20140341909 14/318425 |
Document ID | / |
Family ID | 26918282 |
Filed Date | 2014-11-20 |
United States Patent
Application |
20140341909 |
Kind Code |
A1 |
Pozsgay; Vince |
November 20, 2014 |
CONJUGATION OF BIOMOLECULES USING DIELS-ALDER CYCLOADDITION
Abstract
A method is provided for covalently linking carbohydrates,
proteins, nucleic acids, and other biomolecules under neutral
conditions, using a Diels-Alder cycloaddition reaction. In an
example, activated carbon-carbon double bonds were attached to free
amino sites of a carrier protein, and a conjugated diene was
attached to a carbohydrate hapten. Spontaneous coupling of the
carbohydrate and the protein components under very mild conditions
provided glycoconjugates containing up to 37 carbohydrate hapten
units per carrier protein molecule. The method is also applicable
to the immobilization of biomolecules on gel or solid supports. The
conjugated products are useful as immunogens and as analytical and
diagnostic reagents.
Inventors: |
Pozsgay; Vince; (Washington,
DC) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
The United States of America, as represented by the Secretary,
Department of Health and Human Serv |
Bethesda |
MD |
US |
|
|
Assignee: |
The United States of America, as
represented by the Secretary, Department of Health and Human
Serv
Bethesda
MD
|
Family ID: |
26918282 |
Appl. No.: |
14/318425 |
Filed: |
June 27, 2014 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11724837 |
Mar 16, 2007 |
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14318425 |
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10692411 |
Oct 22, 2003 |
7211445 |
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11724837 |
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09919637 |
Aug 1, 2001 |
6673905 |
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10692411 |
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60223959 |
Aug 9, 2000 |
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Current U.S.
Class: |
424/137.1 ;
424/193.1; 424/197.11; 530/387.5 |
Current CPC
Class: |
A61K 2039/6037 20130101;
A61K 39/385 20130101; A61K 2039/6081 20130101; C08B 37/00 20130101;
Y02A 50/484 20180101; C07K 1/1077 20130101; Y02A 50/476 20180101;
C08B 37/0021 20130101; A61K 47/61 20170801; A61K 2039/62 20130101;
A61K 2039/627 20130101; A61K 47/643 20170801; C12N 11/10 20130101;
C07K 16/44 20130101; A61K 39/0283 20130101; A61K 2039/6068
20130101; Y02A 50/482 20180101; A61K 39/0275 20130101; Y02A 50/30
20180101 |
Class at
Publication: |
424/137.1 ;
530/387.5; 424/193.1; 424/197.11 |
International
Class: |
A61K 39/385 20060101
A61K039/385; A61K 39/112 20060101 A61K039/112; C07K 16/44 20060101
C07K016/44 |
Claims
1-31. (canceled)
32. An antibody which immunoreacts with a polysaccharide, wherein
said antibody is obtained from a mammal, and wherein the production
of the antibody by the mammal has been induced by administering to
said mammal a composition, wherein the composition comprises a
conjugate of biomolecules selected from the group consisting of
##STR00013## wherein R and R' are independently H or methyl, or
together constitute CH.sub.2, CH.sub.2CH.sub.2, or O; X is CH or N;
Y is N, CH.dbd.C, or NH--N; and B.sub.1 and B.sub.2 comprise
biomolecules independently selected from the group consisting of
polypeptides, carbohydrates, polysaccharides, and nucleic acids,
and are optionally attached via a linker wherein one of the
biomolecules is a polysaccharide.
33-36. (canceled)
37. An antibody, produced by a hybridoma, which immunoreacts with a
polysaccharide, wherein nucleic acid sequences encoding said
antibody in said hybridoma are obtained from a mammal in which the
production of the antibody has been induced by administering to
said mammal a composition, wherein the composition comprises a
conjugate of biomolecules selected from the group consisting of
##STR00014## wherein R and R' are independently H or methyl, or
together constitute CH.sub.2, CH.sub.2CH.sub.2, or O; X is CH or N;
Y is N, CH.dbd.C, or NH--N; and B.sub.1 and B.sub.2 comprise
biomolecules independently selected from the group consisting of
polypeptides, carbohydrates, polysaccharides, and nucleic acids,
and are optionally attached via a linker wherein one of the
biomolecules is a polysaccharide.
38-41. (canceled)
42. A method of inducing passive immunity in a mammal, comprising
administering to said mammal an effective amount of an antibody
according to claim 32.
43. A method of inducing passive immunity in a mammal, comprising
administering to said mammal an effective amount of an antibody
according to claim 37.
44-47. (canceled)
48. A method of inducing, in a mammal, antibodies which immunoreact
with a polysaccharide, comprising administering to said mammal a
composition comprising a pharmaceutically acceptable carrier and a
conjugate of biomolecules prepared by (a) covalently attaching a
diene moiety to a first biomolecule to form a diene component; (b)
covalently attaching a dienophile to a second biomolecule to form a
dienophile component; and (c) contacting the diene component with
the dienophile component under conditions that permit a
cycloaddition reaction to occur between the components.
49. A method according to claim 48, wherein the first biomolecule
is a polysaccharide and the second biomolecule is a
polypeptide.
50. The method of claim 49 wherein the polysaccharide is selected
from the group consisting of bacterial capsular polysaccharides,
fragments thereof, and synthetic analogues thereof.
51. The method of claim 49, wherein the bacterial capsular
polysaccharide is selected from the group consisting of capsular
polysaccharides of Haemophilus influenzae type b, Neisseria
meningitides, Group B Streptococci, Salmonella typhi, E. coli, and
Pneumococci.
52. The method of claim 49 wherein the polypeptide is selected from
the group consisting of bacterial toxins, bacterial toxoids,
bacterial outer membrane proteins, keyhole limpet hemocyanin,
horseshoe crab hemocyanin, edestin, mammalian serum albumins,
mammalian gamma-globulins, and IgG-G.
53. An antibody which immunoreacts with a polysaccharide, wherein
said antibody is obtained from a mammal, and wherein the production
of the antibody by the mammal has been induced by the method of
claim 48.
54. An antibody which immunoreacts with a polysaccharide, wherein
said antibody is obtained from a mammal, and wherein the production
of the antibody by the mammal has been induced by the method of
claim 49.
55. An antibody, produced by a hybridoma, which immunoreacts with a
polysaccharide, wherein nucleic acid sequences encoding said
antibody in said hybridoma are obtained from a mammal in which the
production of the antibody has been induced by the method of claim
48.
56. An antibody, produced by a hybridoma, which immunoreacts with a
polysaccharide, wherein nucleic acid sequences encoding said
antibody in said hybridoma are obtained from a mammal in which the
production of the antibody has been induced by the method of claim
49.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This is a divisional of U.S. patent application Ser. No.
11/724,837, filed Mar. 16, 2007, which is a divisional of U.S.
patent application Ser. No. 10/692,411, filed Oct. 22, 2003, issued
as U.S. Pat. No. 7,211,445, which is a divisional of U.S. patent
application Ser. No. 09/919,637, filed Aug. 1, 2001, issued as U.S.
Pat. No. 6,673,905, which claims the benefit of U.S. Provisional
Application No. 60/223,959, filed Aug. 9, 2000. Each of these
applications is incorporated by reference herein in their
entirety.
FIELD OF THE INVENTION
[0002] The invention is in the field of bioorganic chemistry, more
specifically the field of conjugation of biomolecules. The
conjugated products prepared by the methods of the invention are
useful, for example, as inoculants for the generation of
antibodies, and as vaccines. The methods of the invention may also
be used to immobilize biomolecules on solid supports. The
immobilized biomolecules are useful in many fields, such as for
example catalysis, separation, analysis, and diagnostics.
BACKGROUND
[0003] The conjugation of biomolecules to solid and gel supports is
a common operation in many laboratories, and many methods have been
developed for this purpose. Immobilization of enzymes (I.
Chemukhin, E. Klenova, Anal. Biochem. 2000, 280:178-81),
oligonucleotides (J. Andreadis, L. Chrisey, Nucleic Acids Res.
2000, 28:e5; A. Drobyshev et al., Nucl. Acids. Res. 1999,
27:4100-4105), antibodies (P. Soltys, M. Etzel, Biomaterials 2000,
21:37-48), and antigens (M. Oshima, M. Atassi, Immunol. Invest.
1989, 18:841-851) on solid and gel supports enables the preparation
of useful products such as chromatographic media (Meth. Enzym., W.
Jakoby, M. Wilchek, eds., 1974, 34, Academic Press, NY), catalysts
(T. Krogh et al., Anal. Biochem., 1999, 274:153-62), biosensors (J.
Spiker, K. Kang, Biotechnol. Bioeng. 1999, 66:158-63), and numerous
diagnostic (G. Ramsay, Nature Biotechnol., 1998, 16:40-44) and
research tools (C. Bieri et al., Nature Biotechnol. 1999,
17:1105-1108). Even whole cells may be immobilized by such methods
(E. Olivares, W. Malaisse, Int. J. Mol. Med. 2000, 5:289-290).
[0004] The most robust form of attachment of a biomolecule to a
surface or other support is via covalent bonds. Typically, such
bonds are heteroatom-based (e.g., amide, ester, and disulfide
bonds), because such bonds are easily formed under mild conditions.
Non-covalent attachment via specific binding pairs (e.g.,
biotin-avidin or antibody-antigen interactions) is also commonly
employed, but such methods still require initial conjugation of the
specific binding pairs to the biomolecule and support. The use of
carbon-carbon bonds for this purpose is very rare, because
formation of C--C bonds is more difficult, especially under the
mild aqueous conditions appropriate for working with proteins.
[0005] The use of the Diels-Alder reaction to attach a member of a
specific binding pair has been described. In this report (M. N.
Yousaf and M. Mrksich, J. Am. Chem. Soc., 1999, 121:4286), a
Diels-Alder reaction was used to covalently attach a biotinylated
diene to an immobilized dienophile, and the immobilized biotin was
subsequently used to non-covalently immobilize streptavidin. These
workers have more recently used a Diels-Alder reaction to
immobilize the peptide RGDS on a self-assembled alkanethiol
monolayer on a gold surface (M. N. Yousaf, B. T. Houseman, M.
Mrksich, Angew. Chem. Int. Ed. Engl., 2001, 40:1093). The use of
the Diels-Alder reaction to effect the actual covalent coupling or
immobilization event of large biomolecules, however, had not
previously been described.
[0006] The conjugation of biomolecules to one another is likewise a
very common procedure, and is subject to most of the concerns and
limitations described above for biomolecule immobilization.
Covalent attachment of haptens to proteins has been a target of
synthetic endeavors since the discovery by Landsteiner that this
process can convert non-immunogenic molecules to immunogenic
materials (K. Landsteiner, H. Lampl, Biochem. Zeitschr. 1918,
86:343). The application of this concept to carbohydrates by Goebel
and Avery revealed that covalent carbohydrate-protein conjugates
are immunogenic and can generate anti-carbohydrate antibodies (W.
Goebel, J. Exp. Med. 1940, 72:33). The use of Landsteiner's
principle has led to the development of carbohydrate-protein
conjugates that are valuable tools in glycomedical research, and
that are useful as pharmaceuticals. In particular, protein
conjugates of fragments of the capsular polysaccharide of
Haemophilus influenzae type b have become established as successful
vaccines (J. Robbins et al., J. Am. Med. Assoc. 1996, 276:1811).
Several other bacterial saccharide-protein conjugates are in
various stages of clinical studies (E. Konadu et al., J. Infect.
Dis. 1998, 177:383-387; E. Konadu et al., Infect. Immun. 2000,
68:1529-1534) while numerous others are in the pre-clinical phase
(V. Pozsgay et al., Proc. Natl. Acad. Sci. USA 1999, 96:5194).
[0007] The choice of methods for covalent bond formation between
biomolecules such as carbohydrates and proteins is restricted by
their limited solubility in organic solvents, and in many cases by
their pH and temperature sensitivity. In almost all cases, water is
the only solvent that can be used for conjugation of carbohydrates
or proteins, and the conditions are usually limited to temperatures
under 50.degree. C. and pH values between 6 and 8.
[0008] Numerous methods have been developed for the attachment of
polysaccharides to proteins (C. Peeters et al., in Vaccine
Protocols, A. Robinson et al., Eds., 1996 Humana Press, NJ, p. 111;
W. Dick, Jr., M. Beurret, in Contrib. Microbiol. Immunol., J. Cruse
and R. Lewis, eds., 1989, 10:48-114, Karger Basel; H. Jennings, R.
Sood, in Neoglycoconjugates. Preparation and Applications, Y. Lee,
R. Lee, eds., Academic Press, New York, 1994, p. 325). However,
only a few of these methods are capable of coupling
oligosaccharides to carriers in a site-selective fashion. Most
prominent among these is reductive amination, which converts the
reducing-end residue of the polysaccharide into a polyhydroxy
alkylamino moiety, which unfortunately causes the loss of this unit
as a true carbohydrate in the resulting glycoconjugate (V. Pozsgay,
Glycoconjugate J. 1993, 10:133).
[0009] This problem can be solved by chemical synthesis of
oligosaccharide glycosides with aglycons that bear a (latent)
reactive group. Examples include alkenyl groups (M. Nashed,
Carbohydr. Res. 1983, 123:241-246; J. Allen, S. Danishefsky, J. Am.
Chem. Soc. 1999, 121:10875), 3-aminoproply (G. Veeneman et al.,
Tetrahedron 1989, 45:7433), 4-aminophenylethyl (R. Eby, Carbohydr.
Res. 1979, 70:75), 4-aminophenyl (S. Stirm et al., Justus Liebigs
Ann. Chem. 1966, 696:180), 6-aminohexyl (J. Hermans et al., Rec.
Tray. Chim. Pays-Bas 1987, 106:498; R. Lee et al., Biochemistry
1989, 28:1856), 5-methoxycarbonylpentyl (S. Sabesan, J. Paulson, J.
Am. Chem. Soc. 1986, 108:2068; V. Pozsgay, Org. Chem. 1998,
63:5983), 8-methoxycarbonyloctyl (R. Lemieux et al., J. Am. Chem.
Soc. 1975, 97, 4076; B. Pinto et al., Carbohydr. Res. 1991, 210,
199), 4-aminobenzyl (W. Goebel, J. Exp. Med. 1940, 72:33),
.omega.-aldehydroalkyl (V. Pozsgay, Glycoconjugate J. 1993,
10:133), 3-(2-aminoethylthio)propyl (Y. Lee, R. Lee, Carbohydr.
Res. 1974, 37:193), 2-chloroethylthioglycosides (M. Ticha et al.,
Glycoconjugate J. 1996, 13:681), and 1-O-succinimide derivatives
(M. Anderson, S. Oscarson, Bioconjugate Chem. 1993, 4:246; B.
Davis, J. Chem. Soc. Perkin I 1999, 3215).
[0010] These aglycons introduce spacers that can be linked to a
protein either directly or after insertion of a secondary linker.
For this purpose the use of an activated dicarboxylic acid has been
reported (R. van den Berg et al., Eur. J. Org. Chem. 1999,
2593-2600). In another procedure, a sulfhydryl group at the
terminal position of the spacer allows the formation of a disulfide
bridge with proteins using the dithiopyridyl method (J. Evenberg et
al., J. Infect. Dis. 1992, 165(sup. 1):S152). In a related
protocol, a thiolated protein is coupled with a
maleimido-derivatized saccharide (J. Mahoney, R. Schnaar, Methods
Enzymol. 1994, 242:17). N-acryloylamidophenyl glycosides may be
coupled to unmodified proteins using a Michael addition (A.
Romanowska et al., Methods Enzymol. 1994, 242:90). As an
alternative to glycoside formation, direct coupling of a
carbohydrate to a linker via amide bonds has also been used (A.
Fattom et al., Infect. Immun. 1992, 60:584-589), but this approach
is limited to carboxyl-containing carbohydrates.
[0011] The yields of any of these methods rarely exceed 40%, and
are generally in the 10-20% range (R. van den Berg et al., Eur. J.
Org. Chem. 1999, 2593-2600), especially when medium or high
carbohydrate loading in the conjugate is attempted. This problem is
compounded by the fact that the oligosaccharide haptens, usually
obtained in multistep syntheses or by controlled degradation of
polysaccharides, can rarely be recovered in their active or
activable form after the coupling procedure. An additional problem
with most chemical coupling methods employed to date is the
formation of cross-linked byproducts, due to the presence of
multiple reactive functional groups (e.g., amines, acids,
hydroxyls, and sulfhydryls) on most biomolecules. Avoidance of this
problem requires that the reactive groups be blocked, which
requires additional processing steps and may alter the
physiochemical and immunological properties of the biomolecule.
Thus, there remains a need for a mild and site-selective method for
coupling biomolecules to one another, which avoids the problems of
low yields, crosslinking, and loss of starting materials. For
similar reasons there remains a need for mild and selective methods
for attaching biomolecules to surfaces and solid and gel
supports.
BRIEF DESCRIPTION OF THE DRAWINGS
[0012] FIG. 1 illustrates the increase in molecular weight as a
function of time of a conjugate (conjugate 28) of the invention
relative to the mass of the core protein with the dienophiles
attached.
[0013] FIG. 2 illustrates a similar time-dependent experiment as
represented in FIG. 1, conducted with the same
maleimido-derivatized protein, using previously-described diene
8.
BRIEF DESCRIPTION OF THE INVENTION
[0014] The present invention provides an experimentally simple
protocol for the covalent attachment of biomolecules to one another
and to supports that can avoid many of the above-mentioned
problems. The invention makes use of the well-known Diels-Alder
cycloaddition reaction that takes place between a double bond and a
conjugated diene. This reaction has traditionally been carried out
in organic solvents, but can proceed in aqueous solutions as well
(R. Breslow, D. Rideout, J. Am. Chem. Soc. 1980, 102:7816; A.
Lubineau, J. Auge, Top Curr Chem 1999, 206:1; P. Garner, in Organic
Synthesis in Water, P. Grieco, ed., Blackie Academic and
Professional, London, 1998, p. 1).
[0015] Carbohydrates have been employed as chiral auxiliaries
and/or water solubilizing agents for Diels-Alder reactions, wherein
a conjugated diene system is converted to a glycoside prior to the
cycloaddition (A. Lubineau et al., J. Chem. Soc. Perkin 1 1997,
2863-2867; see also S. Pellegrinet, R. Spanevello, Org. Lett. 2000,
2:1073-1076). As noted above, the Diels-Alder reaction has also
been used to covalently attach biotin to a support (M. N. Yousaf
and M. Mrksich, J. Am. Soc. 1999, 121:4286). However, the
Diels-Alder reaction has not previously been extended to the direct
covalent conjugation of biopolymers or other types of polymeric
materials. Among the advantages of the method of the invention are
the mild and neutral conditions, good yields, negligible
cross-linking, and facile recovery of excess and/or unreacted
biomolecules in their conjugatable form.
[0016] The invention also provides conjugated biomolecules, which
are useful as immunostimulatory agents for production of antibodies
and induction of immunity, methods of inducing antibody production
with the conjugated biomolecules, and vaccine compositions
comprising the conjugated biomolecules.
[0017] The invention also provides polyclonal and monoclonal
antibodies generated by administration of the conjugated
biomolecules to a mammal, and methods of using the induced
antibodies for inducing passive immunity. The antibodies are useful
of therapeutic, diagnostic, and analytical purposes.
[0018] The invention also provides immobilized biomolecules, and
methods for their preparation, which are useful in many areas, such
as chromatographic media, catalysts, components of diagnostic
devices, biosensors, and as research tools.
DETAILED DESCRIPTION OF THE INVENTION
[0019] The invention provides a new method for conjugation of
biomolecules based on Diels-Alder cycloaddition reaction. The
technique involves the introduction of an activated double bond
into a first biomolecule component, and a conjugated diene into a
second biomolecule component, which are to be covalently linked
together. The diene- and dienophile-modified biomolecules may then
be purified to the extent desired. The two components are then
simply combined under neutral conditions, and the cycloaddition
reaction is allowed to proceed.
[0020] As used herein, the term "biomolecule" refers generally to
the large, complex molecules produced within living cells, and to
synthetic and semi-synthetic analogues thereof. Examples include
proteins, peptides, oligo- and poly-saccharides, and oligo- and
poly-nucleic acids, and various combinations thereof such as for
example glycoproteins and nucleoproteins. Larger complexes, such as
ribosomes, cellular substructures, and even entire cells are
intended to fall within the meaning of the term as well.
[0021] In certain of the examples presented, the diene moiety is
introduced into a carbohydrate component and the activated double
bond into a polypeptide component. This may be reversed if desired,
as a matter of convenience or if required by the synthetic design,
as shown in another of the examples. The cycloaddition step
proceeds in most cases under neutral conditions, at or below
physiological temperatures. Where they are of sufficiently low
molecular weight, unreacted components can be recovered for re-use
by simple diafiltration of the coupling reaction mixture.
[0022] The embodiment of the invention disclosed in certain of the
examples below incorporates an electron-deficient carbon-carbon
double bound into a protein, with human serum albumin (HSA) being
used as an example and the commercially available reagent
3-sulfosuccinimidyl 4-maleimidobutyrate being used as the reagent.
In these embodiments, the diene component of the Diels-Alder
reaction is incorporated into a carbohydrate, with derivatives of
trans,trans-hexa-2,4-dien-1-ol, 1-amino-hexa-2,4-diene, and
octa-2,4-dienoic acid hydrazide being used as dienes. It will be
understood that in general, the Diels-Alder reaction will occur
between sterically accessible dienes and dienophiles regardless of
the nature of the attached biomolecules, and that by appropriate
selection of reagents both homoconjugates and heteroconjugates of
proteins, carbohydrates, and oligonucleotides can be carried out by
the methods of this invention.
[0023] The methods of the invention unexpectedly provide for a
degree of control over the rate of coupling of the diene and
dienophile components, simply by modification of the linker
moieties. The molecular weight of the conjugate approaches a
limiting value as the coupling reaction proceeds, which depends on
the mass of the carbohydrate being attached. The observed increase
in molecular weight as a function of time may therefore be fit to a
pseudo-first order reaction kinetic equation of the form
.DELTA.mw=.DELTA.mw.sub.max(1-e.sup.-kt)
where .DELTA.mw is the increase in molecular weight,
.DELTA.mw.sub.max is the maximum increase that could be obtained if
all the protein linked starting material were to react, k is the
rate constant and t is time. The curves drawn through the data of
FIGS. 1 and 2 (see Example 5 for the experimental details) were
obtained by fitting the parameters of this equation to the data.
The results of this fitting are summarized in Table 2.
[0024] The faster incorporation of construct 27 relative to 8 is an
interesting observation. The observed difference apparently
correlates with the distance between the hydrophilic rhamnose
moiety and the hydrophobic diene part of the molecule. According to
Breslow, the accelerating effect of water on the rate of
Diels-Alder reactions is due to hydrophobic packing of the
reactants (R. Breslow, D. Rideout, J. Am. Chem. Soc. 1980,
102:7816). Such an effect should be more pronounced in compound 27,
where the diene sector is more isolated from the hydrophilic
portion, than in compound 8.
[0025] The Diels-Alder reaction requires a highly organized
transition state, and the ability of the components of the present
invention to achieve the proper geometry for cycloaddition despite
the mass and bulk of the attached biomolecules is remarkable.
[0026] In a preferred embodiment of the invention, one of the
biomolecules to be linked is a hapten or antigen, and the other is
a carrier. In a particularly preferred embodiment, the hapten or
antigen is a polysaccharide moiety. Examples of antigenic
polysaccharides are the capsular polysaccharides of Haemophilus
influenzae type b, Neisseria meningitidis, Group B Streptococci,
Salmonella typhi, E. coli, and Pneumococci.
[0027] Carriers are chosen to increase the immunogenicity of the
hapten or antigen, and/or to raise antibodies against the carrier
itself which may be medically beneficial. Carriers that fulfill
these criteria are known in the art (see, e.g., A. Fattom et al.,
Infect. Immun. 1990, 58:2309-2312; Devi, J. Robbins, R. Schneerson,
Proc. Natl. Acad. Sci. USA 1991, 88:7175-7179; S. Szu, X et al.,
Infect. Immun. 1991, 59:4555-4561; S. Szu et al., J. Exp. Med.
1987, 166:1510-1524). A carrier can be a natural, semi-synthetic,
or synthetic material containing one or more functional groups, for
example primary and/or secondary amino groups, azido groups,
hydroxyl groups, or carboxyl groups, to which a diene or dienophile
Diels-Alder reactant moiety can be attached. The carrier can be
water soluble or insoluble, and is preferably a polypeptide.
[0028] Examples of water soluble polypeptide carriers include, but
are not limited to, natural, synthetic, or semisynthetic peptides
or proteins from bacteria or viruses, e.g., bacterial, bacterial
outer membrane proteins, bacterial toxins and toxoids such as
tetanus toxin/toxoid, diphtheria toxin/toxoid, Pseudomonas
aeruginosa exotoxin/toxoid/protein, pertussis toxin/toxoid, and
Clostridium perfringens exotoxins/toxoids. Viral proteins such as
hepatitis B surface antigen and core antigen may also be used as
carriers, as well as proteins from higher organisms such as keyhole
limpet hemocyanin, horseshoe crab hemocyanin, edestin, mammalian
serum albumins, mammalian gamma-globulins, and IgG.
[0029] Polysaccharide carriers include, but are not limited to,
dextran, capsular polysaccharides from microorganisms such as the
Vi capsular polysaccharide from S. typhi, which is described in
U.S. Pat. No. 5,204,098 (incorporated by reference herein);
Pneumococcus group 12 (12F and 12A) polysaccharides; Haemophilus
influenzae type d polysaccharide; and certain plant, fruit, or
synthetic oligo- or polysaccharides which are immunologically
similar to capsular polysaccharides, such as pectin,
D-galacturonan, oligogalacturonate, or polygalacturonate, for
example as described in U.S. Pat. No. 5,738,855 (incorporated by
reference herein).
[0030] Examples of water soluble carriers include, but are not
limited to, aminoalkyl agarose, e.g., aminopropyl or aminohexyl
SEPHAROSE (Pharmacia Inc., Piscataway, N.J.), aminopropyl glass,
cross-linked dextran, and the like, to which a diene or dienophile
can be attached. Other carriers may be used provided that a
functional group is available for covalently attaching a diene or
dienophile.
[0031] Examples of dienophiles include, but are not limited to,
maleimides, acrylamides, azodicarboxylates, quinones, and
1,2,4-triazoline-3,5-diones. Examples of dienes include, but are
not limited to, esters and glycosides of hexa-2,4-dien-1-ol,
penta-2,4-dien-1-ol, furan-2-methanol, and furan-1-methanol;
esters, amides, and hydrazides of octa-2,4-dienoic acid; and amides
of 1-aminohexa-2,4-diene and 1- and 2-aminomethylfuran. The
above-mentioned amines may also be coupled with
aldehydro-biomolecules via reductive amination, and hydrazides may
be attached to such biomolecules via condensation.
[0032] The invention also provides biomolecule conjugates of
general formulas I and II below:
##STR00001##
where R and R' are independently H or methyl, or together
constitute CH.sub.2, CH.sub.2CH.sub.2, SO.sub.2, or O; X is CH or
N; Y is N, CH.dbd.C, or NH--N; and B.sub.1 and B.sub.2 comprise
biomolecules independently selected from the group consisting of
polypeptides, carbohydrates, polysaccharides, and nucleic acids,
and are optionally attached via a linker.
[0033] The invention also provides immobilized biomolecules of
formulas I and II above, where one of B.sub.1 and B.sub.2 may be a
solid or gel support. Examples of solid supports include, but are
not limited to, aminopropylsilylated glass and silica surfaces,
gold surfaces functionalized with thiol-bound linkers,
functionalized macroporous polystyrene beads, and
surface-derivatized microtiter plate wells. Examples of gel
supports include, but are not limited to, functionalized agarose
gels such as cyanogen bromide activated agarose, aminoethyl
agarose, and carboxymethyl agarose.
[0034] The formulas above are intended to indicate that the group
B.sub.1 may be attached alpha or beta to the group R' as shown
below:
##STR00002##
[0035] There are many known methods of attachment of small
molecules to biomolecules, there are many known linker moieties for
attachment of chemical moieties to biomolecules, and there are many
known dienes and dienophiles that readily take part in
cycloaddition reactions at or near room temperature. Those skilled
in the art will thus appreciate that there are many obvious
combinations of attachment methods, linkers, and diene and
dienophile partners that may be employed in the method of
biomolecule coupling disclosed herein, which are equivalent to the
examples provided. Such modifications of the disclosed methods and
resulting compositions are intended to be within the scope and
spirit of the present invention.
[0036] It is another object of the invention to provide methods of
using the polysaccharide-carrier conjugates of this invention for
eliciting an immunogenic response in mammals, including but not
limited to responses which provide protection against, or reduce
the severity of, bacterial and viral infections. The pharmaceutical
compositions of this invention are expected to be capable, upon
injection into a mammal, of reducing serum antibodies against the
polysaccharide component of the conjugate.
[0037] The invention also provides methods of using such
conjugates, and/or pharmaceutical compositions comprising such
conjugates, to induce in mammals, in particular, humans, the
production of antibodies which immunoreact with the polysaccharide
component of the conjugates. Antibodies which immunoreact with a
bacterial or viral polysaccharide are useful for the identification
or detection of microorganisms expressing the polysaccharide,
and/or for diagnosis of infection. Antibodies against the
polysaccharide may be useful in increasing resistance to,
preventing, ameliorating, and/or treating illnesses caused by
microorganisms or viruses that express the polysaccharide.
[0038] The compositions of this invention are intended for active
immunization for prevention of infection, and for preparation of
immune antibodies. The compositions of this invention are designed
to induce antibodies specific to microorganisms expressing the
polysaccharide component of the conjugate, and to confer specific
immunity against infection with such microorganisms.
[0039] This invention also provides compositions, including but not
limited to, mammalian serum, plasma, and immunoglobulin fractions,
which contain antibodies which are immunoreactive with the
polysaccharide component of the conjugates of this invention, and
which preferably also contain antibodies which are immunoreactive
with the protein component. These antibodies and antibody
compositions may be useful to prevent, treat, or ameliorate
infection and disease caused by the microorganism. The invention
also provides such antibodies in isolated form. The invention
further provides methods of inducing in mammals antibodies which
immunoreact with a polysaccharide, the methods comprising
administering to a mammal a composition of the invention.
[0040] The invention also provides monoclonal antibodies,
preferably produced by hybridomas, which immunoreact with a
polysaccharide. The nucleic acid sequences encoding these
antibodies are obtained from a mammal in which the production of
anti-polysaccharide antibodies has been induced by administering a
composition of the invention.
[0041] As used herein, the terms "immunoreact" and
"immunoreactivity" refer to specific binding between an antigen or
antigenic determinant-containing molecule and a molecule having an
antibody combining site, such as a whole antibody molecule or a
portion thereof.
[0042] As used herein, the term "antibody" refers to immunoglobulin
molecules and immunologically active portions of immunoglobulin
molecules. Exemplary antibody molecules are intact immunoglobulin
molecules, substantially intact immunoglobulin molecules and
portions of an immunoglobulin molecule, including those portions
known in the art as Fab, Fab', F(ab').sub.2 and F(v), as well as
chimeric antibody molecules.
Polymeric Carriers
[0043] Carriers are chosen to increase the immunogenicity of the
polysaccharide and/or to raise antibodies against the carrier which
are medically beneficial. Carriers that fulfill these criteria are
well-known in the art. A polymeric carrier can be a natural or a
synthetic material containing one or more functional groups, for
example primary and/or secondary amino groups, azido groups, or
carboxyl groups, to which a diene or dienophile component can be
attached. Carriers can be water soluble or insoluble. The examples
below employ proteins as carriers.
[0044] Regardless of the precise method used to prepare the
conjugate, after the Diels-Alder coupling reaction has been carried
out the unreacted materials are preferably removed by routine
physicochemical methods, such as for example dialysis, gel
filtration or ion exchange column chromatography, depending on the
materials to be separated. The final conjugate consists of the
polysaccharide and the carrier bound through a Diels-Alder
adduct.
Dosage for Vaccination
[0045] The present inoculum contains an effective, immunogenic
amount of a polysaccharide-carrier conjugate. The effective amount
of polysaccharide-carrier conjugate per unit dose sufficient to
induce an immune response depends, among other things, on the
immunogenicity of the polysaccharide, the species of mammal
inoculated, the body weight of the mammal, and the chosen
inoculation regimen, as is well known in the art. Inocula typically
contain polysaccharide-carrier conjugates with concentrations of
polysaccharide from about 1 microgram to about 500 micrograms per
inoculation (dose), preferably about 3 micrograms to about 50
micrograms per dose, and most preferably about 5 micrograms to 25
micrograms per dose.
[0046] The term "unit dose" as it pertains to the inocula refers to
physically discrete units suitable as unitary dosages for mammals,
each unit containing a predetermined quantity of active material
(polysaccharide) calculated to produce the desired immunogenic
effect in association with the required diluent.
[0047] Inocula are typically prepared in physiologically and/or
pharmaceutically tolerable (acceptable) carriers, and are
preferably prepared as solutions in physiologically and/or
pharmaceutically acceptable diluents such as water, saline,
phosphate-buffered saline, or the like, to form an aqueous
pharmaceutical composition. Adjuvants, such as aluminum hydroxide,
QS-21, TiterMax.TM. (CytRx Corp., Norcross, Ga.), Freund's complete
adjuvant, Freund's incomplete adjuvant, interleukin-2, thymosin,
and the like, may also be included in the compositions.
[0048] The route of inoculation may be by intramuscular or
subcutaneous injection or the like, so long as it results in
eliciting antibodies reactive against the polysaccharide component.
It is anticipated that in some cases the composition can be
administered orally or intranasally, for example when mucosal
immunity is to be induced. In order to increase the antibody level,
a second or booster dose may be administered approximately 4 to 6
weeks after the initial administration. Subsequent doses may be
administered as deemed necessary by the practitioner.
Antibodies
[0049] An antibody of the present invention is typically produced
by immunizing a mammal with an immunogen or vaccine containing a
polysaccharide-carrier conjugate, preferably a
polysaccharide-protein conjugate, to induce in the mammal antibody
molecules having immunospecificity for the polysaccharide component
of the conjugate. Antibody molecules having immunospecificity for
the protein carrier may also be produced. The antibody molecules
may be collected from the mammal and, optionally, isolated and
purified by methods known in the art.
[0050] Human or humanized monoclonal antibodies are preferred,
including but not limited to those identified by phage display
technology, and including but not limited to those made by
hybridomas and by mice with human immune systems or human
immunoglobin genes. The antibody molecules of the present invention
may be polyclonal or monoclonal. Monoclonal antibodies may be
produced by methods well-known in the art. Portions of
immunoglobulin molecules, such as Fabs, may also be produced by
methods known in the art.
[0051] An antibody of the present invention may be contained in
blood plasma, serum, hybridoma supernatants and the like.
Alternatively, the antibodies of the present invention are isolated
to the extent desired by well known techniques such as, for
example, ion chromatography or affinity chromatography. The
antibodies may be purified so as to obtain specific classes or
subclasses of antibody such as IgM, IgG, IgA, IgG.sub.1, IgG.sub.2,
IgG.sub.3, IgG.sub.4 and the like. Antibodies of the IgG class are
preferred for conferring passive immunity. The antibodies of the
present invention have a number of diagnostic and therapeutic uses.
The antibodies can be used as an in vitro diagnostic agents to test
for the presence of microorganisms in biological samples or in
water or food samples, in standard immunoassay protocols. Such
assays include, but are not limited to, agglutination assays,
radioimmunoassays, enzyme-linked immunosorbent assays, fluorescence
assays, Western blots and the like. In one such assay, for example,
the sample is contacted with first antibodies of the present
invention, and a labeled second antibody is used to detect the
presence of polysaccharides to which the first antibodies have
bound.
[0052] Such assays may be, for example, of direct format (where the
labeled first antibody is reactive with the polysaccharide), an
indirect format (where a labeled second antibody is reactive with
the first antibody), a competitive format (such as the addition of
a labeled polysaccharide), or a sandwich format (where both labeled
and unlabelled antibody are utilized), as well as other formats
described in the art.
[0053] In providing the antibodies of the present invention to a
recipient mammal, the dosage of administered antibodies will vary
depending upon such factors as the mammal's age, weight, height,
sex, general and specific medical conditions, and the like.
[0054] In general, it is desirable to provide the recipient with a
dosage of antibodies which is in the range of from about 1 mg/kg to
about 10 mg/kg body weight of the mammal, although a lower or
higher dose may be administered. The antibodies of the present
invention are intended to be provided to the recipient subject in
an amount sufficient to prevent, or lessen or attenuate the
severity, extent or duration of the infection.
[0055] In order to facilitate the administration of the conjugates
of the invention to mammals, it is preferred that the conjugate be
formulated with a pharmaceutically acceptable carrier. (Those
skilled in the art will appreciate that the term "carrier," when
used in this context, has a different meaning than when it is used
to refer to a biomolecule component of the conjugate.) Examples of
pharmaceutically acceptable carriers include sterile water and
saline, both of which may be buffered with phosphate, citrate, and
the like. The conjugates of the invention may be provided in
solution or suspension in a pharmaceutically acceptable carrier, or
they may be provided in dry form and reconstituted with the
pharmaceutically acceptable carrier prior to administration.
[0056] The administration of the conjugates and compositions of the
invention may be for prophylactic or therapeutic purposes. When
provided prophylactically, the agents are provided in advance of
any symptom. The prophylactic administration of the agent serves to
prevent or ameliorate any subsequent infection. When provided
therapeutically, the agent is provided at (or shortly after) the
onset of a symptom of infection.
[0057] For all therapeutic, prophylactic and diagnostic uses, the
polysaccharide-carrier conjugates of this invention, as well as
antibodies and other necessary reagents and appropriate devices and
accessories may be provided in kit form so as to be readily
available and easily used.
[0058] The examples below will be understood to be merely
representative of the invention, and are not intended to limit the
scope of the appended claims in any way.
##STR00003##
Example 1
[0059] Dieneophile Component 2:
[0060] Treatment of human serum albumin (HSA) with a 1.6 molar
excess (based on 58 available amino groups) of 3-sulfosuccinimidyl
4-maleimidobutyrate (1) in a pH 7.5 phosphate buffer afforded the
intermediate 2, which contained an average of 38 maleimido moieties
per protein molecule, as indicated in the formula (determined by
MALDI-TOF mass spectroscopy).
##STR00004##
[0061] Diene Component 8:
[0062] The phenylthio rhamnoside 3 was prepared as described
previously (V. Poszgay, Carbohydr. Res. 1992, 235-295). Rhamnoside
3 was treated with acetic anhydride and pyridine to afford 4,
.sup.1H NMR (CDCl.sub.3, .delta.) 8.11-7.26 (m, 15H), 5.79 (dd,
1H), 5.64-5.52 (m, 3H) 5.53 (t, 1H, J=10.0 Hz), 4.49 (dq, 1H), 1.89
(s, 2 J=6.3 Hz), .sup.13C (CDCl.sub.3, .delta.) 170.1, 165.7,
165.5, 133.5-127.9, 85.8, 72.1, 71.9, 69.5, 68.1, 20.6, 17.6.
[0063] From 4 the hemiacetal 5 was obtained by hydrolysis with
mercuric trifluoroacetate (L. Yan, D. Kahne, J. Am. Chem. Soc.
1996, 118:9239), .sup.1H NMR (CDCl.sub.3, .delta.) 8.1-7.4 (m,
10H), 5.71 (dd, 1H, J=9.9 Hz), 5.78 (dd, 1H), 5.49 (t, 1H, J=9.9
Hz), 5.38 (br d, 1H), .sup.13C(CDCl.sub.3, .delta.) 170.3, 165.8,
92.2, 71.9, 71.0, 68.9, 66.7, 20.7, 17.7.
[0064] Hemiacetal 5 was converted to the trichloroacetimidate 6,
and glycosylation of trans,trans-hexa-2,4-dien-1-ol with 6 using
CF.sub.3SO.sub.3Si(CH.sub.3).sub.3 as the activator afforded the
glycoside 7. The acetyl groups were then removed by treatment with
NaOMe to afford the diene rhamnoside 8. .sup.1H NMR (D.sub.2O,
.delta.) 6.33 (dd, 1H, J=9.5 Hz, J=10.2 Hz), 6.16 (ddd, 1H, J=1.6
Hz, J=10.2 Hz, J=14.8 Hz), 5.85 (m, 1H), 5.69 (m, 1H), 4.83 (d, 1H,
J=1.7 Hz), 4.21 (dd, 1H, J=6.4 Hz, J=12.4 Hz), 4.07 (dd, 1H, J=7.1
Hz, J=12.4 Hz), 3.91 (dd, 1H, J=1.7 Hz, J=3.4 Hz), 3.69 (dq, 1H),
3.73 (dd, 1H, J=3.4 Hz, J=9.6 Hz), 3.44 (t, 1H, J=9.6 Hz), 1.75 (d,
3H, J=6.8 Hz), 1.28 (d, 3H, J=6.3 Hz), .sup.13C (D.sub.2O,
.delta.), 136.7, 132.7, 131.1, 125.6, 99.8, 72.8, 71.1, 71.0, 70.9,
69.4, 68.6, 18.2, 17.4.
##STR00005##
[0065] Coupling Reaction:
[0066] An excess of 8 was treated in an aqueous solution with the
maleimido-derivatized protein 2. The average incorporations of the
hapten, as a function of time and temperature, are shown in Table
1. This data was obtained from the average molecular mass of the
conjugates determined by the MALDI-TOF method. As expected for a
concerted cycloaddition reaction, the incorporation level depends
on the reaction time and temperature. At room temperature,
approximately 63% of the available dienophile moieties in the
protein participated in adduct formation within 36 h, while at
40.degree. C. almost complete utilization of these moieties
occurred after four days (Table 1). The unreacted diene 8 was
recovered by diafiltration, and the conjugate 9 (free amino groups
not shown) was then obtained as a white solid after
freeze-drying.
TABLE-US-00001 TABLE 1 Time and temperature dependence of the
cycloaddition between 2 and 8 Compositions of the conjugate (mol
hapten/mol albumin) Time (h) 22.degree. C. 40.degree. C. 36 24 27
100 28 37
Example 2
[0067] Dienophile Component:
[0068] The glycoside of 6-hydroxyhexanoic acid hydrazide with the
tetramer of
(.alpha.-L-rhamnopyranosyl)-(1-2)-(.alpha.-D-galactopyranosyl)-(1.fwda-
rw.3)-(.alpha.-D-glucopyranosyl)-(1.fwdarw.3)-(.alpha.-L-rhamnopyranose
is prepared, according to the procedure disclosed in international
patent application WO 99/03871. Treatment with maleic anhydride
provides an N-terminal maleimide derivative 10.
[0069] Diene Component:
[0070] Trans, trans-hexa-2,4-dien-1-ol and succinic anhydride are
reacted in the presence of N,N-dimethylaminopyridine to provide
trans,trans-hexa-2,4-dien-1-ol monosuccinate. An aqueous solution
of an excess of the monosuccinate is activated with
1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide and coupled with
human serum albumin, to provide a poly(diene) derivative 11.
##STR00006##
[0071] Coupling Reaction:
[0072] An aqueous solution of 11 and excess 10 is incubated at
35.degree. C. for 4 days, and the resulting conjugate 12 (n<58)
is purified by diafiltration and lyophilized. The conjugate 12 is
expected to be used for inducing antibodies against Shigella
dysenteriae.
Example 3
[0073] Diene Component:
[0074] The Vi capsular polysaccharide of Salmonella typhi (Pasteur
Merieux Serums et Vaccins, Lyon, FR) is dissolved in water, excess
2,4-hexadienylamine is added, and the solution buffered to pH 5.0.
A slight excess of 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide
in water is added. After 4 h at 370, the mixture is adjusted to pH
7.5 and dialyzed to remove excess reagents. The resulting solution
of 13 is used immediately.
[0075] Dienophile Component:
[0076] Pseudomonas aeruginosa recombinant exoprotein A (Fattom et
al., Infect. Immun. 1992, 60:584-589) is treated with excess
3-sulfosuccinimidyl 4-maleimidobutyrate (1) as in Example 1,
followed by dialysis and lyophilization to provide a maleimide
derivatized protein 14.
##STR00007##
[0077] Coupling Reaction:
[0078] An excess of the diene 14 is added to the solution of
dienophile component 13. After 4 days at 37.degree. C., the mixture
is concentrated, and conjugate 15 is purified by size exclusion
chromatography on SEPHACRYL S-1000.TM. (Pharmacia, Piscataway
N.J.). The conjugate 15 is expected to be useful for inducing
antibodies against Salmonella typhi.
Example 4
##STR00008##
[0080] Diene Component:
[0081] To a solution of methyl octa-4,6-dienoate (16) (6 g) in
methanol (10 ml) was added hydrazine (3 ml) at room temperature.
After 24 h, the solution was diluted with water (50 ml). The
crystalline precipitate was isolated by filtration to afford
octa-4,6-dienoic acid hydrazide (17) as colorless microcrystals,
yield 5.7 g. Dextran (nominal MW 10 kDa, Pharmacia) was diafiltered
through a YM10 membrane (MW cutoff 10 kDa, Millipore) using 3
changes of water. The solution that passed through the membrane was
diafiltered through a YM3 (MW cutoff 3000 Da) membrane, using five
changes of water. The material retained by the membrane was
lyophilized. A stirred solution of the dextran 18 thus obtained
(22.5 mg 2.25 .mu.mol, corresponding to 139 .mu.mol of glucose) in
H.sub.2O (2.2 ml) at 5.degree. C. (ice bath) was equipped with a
temperature-sensing pH electrode, and 0.1 M NaOH was added with a
100 .mu.L microsyringe until the pH reached 10.5. To this solution
was added BrCN (50 .mu.L of a 5 M solution in acetonitrile). The pH
of the solution was maintained between 10.5 and 10.8 by addition of
0.1 M NaOH with a microsyringe. After 6 min, the solution was
adjusted to pH 8.5 by addition of pH 8.0 phosphate buffer (ca. 1
ml). To the reaction mixture was immediately added a solution of
octa-4,6-dienoic acid hydrazide (3.5 mg, 25 .mu.mol) in 0.5 ml
dimethyl sulfoxide. The pH of the reaction mixture rose to 8.95, at
a temperature of 9.degree. C. The ice bath was removed and the
stirred solution was allowed to reach 25.degree. C. After 2.5 h,
the pH was 8.18 and remained unchanged over 5 min. The solution of
20 was diafiltered through a YM-3 membrane using 5 changes of water
(4 ml each).
[0082] Dienophile Component:
[0083] A stirred solution of human serum albumin (11.4 mg, 0.17
.mu.mol, 9.88 .mu.mol of amino groups) in pH 7.5 buffer (1 ml) was
treated with 5.degree. C. (ice-bath) with 3-sulfosuccinimidyl
4-maleimidobutyrate (1) (0.52 mg 1.36 .mu.mol). The solution was
stirred for 15 min at 5.degree. C. then for another 15 min at room
temperature followed by diafiltration through a YM-10 membrane
using 5 changes of water (4 ml each).
[0084] Coupling Reaction:
[0085] The residual solutions containing the modified dextran 20
and the modified human serum albumin were combined. The total
volume of the combined solution was approx. 1 ml. After 22 h at
room temperature, the reaction mixture was diafiltered through a
YM-10 membrane using 6 changes of H.sub.20 (5 ml each), and the
residue was freeze-dried. MALDI mass spectroscopy showed that most
of the albumin is consumed. The conjugate 21 that is formed has
average molecular weight of 90 kDa.
Example 5
##STR00009##
[0087] Diene Component:
[0088] 6-Hydroxyhexanoic acid 23 (S. Sabesan, J. C. Paulson, J. Am.
Chem. Soc., 1986, 108:2068) and glycosyl chloride 22 (V. Pozsgay,
Glycoconj. J., 1993, 10:133), in methylene chloride at -40.degree.
C., were treated with silver triflate and
2,6-di-t-butyl-4-methylpyridine for 10 min. to afford glycoside 24.
Deprotection with a catalytic amount of NaOMe in MeOH at 23.degree.
C. for 24 hr, followed by treatment with ethylenediamine in ethanol
at 80.degree. C. for 12 hr, provided 25 in 74% overall yield.
.sup.1H NMR (500 MHz, CD.sub.3OD): .delta.=4.64 (br s, 1H), 3.78
(dd, 1H), 3.66 (m, 1H), 3.55 (dq, 1H), 3.39 (m, 1H), 3.35 (t, 1H),
3.36 (t, 2H), 2.76 (t, 2H), 2.21 (t, 2H), 1.54-1.68 (m, 4H),
1.35-1.46 (m, 2H), 1.24 (d, 3H).
[0089] Octadienoic acid 26 was prepared from the corresponding
methyl ester (T. Hudlicky et al., J. Org. Chem. 1980, 45:5020).
Acylation of 25 with 26 (dicyclohexyl-carbodiimide, EtOAc, MeOH)
afforded the glycoside diene 27 in 92% yield. .sup.1H NMR (500 MHz,
CD.sub.3OD): .delta.=6.09 (m, 2H), 5.70 (m, 1H), 5.57 (m, 1H), 4.77
(br s, 1H), 3.92 (dd, 1H), 3.63-3.75 (m, 4H), 3.53 (m, 1H), 3.43
(t, 1H), 3.32 (m, 4H), 2.32 (m, 4H), 2.22 (m, 2H), 1.72 (d, 3H),
1.53-1.67 (m, 4H), 1.30-1.42 (m, 2H), 1.29 (d, 3H).
[0090] Dienophile Component:
[0091] A stirred solution of human serum albumin in pH 7.5 buffer
(1 ml) was treated at 5.degree. C. (ice-bath) with
3-sulfosuccinimidyl 4-maleimidobutyrate (1) as described above, to
provide maleimido-derivatized protein containing an average of 29
maleimido groups per molecule of protein.
##STR00010##
[0092] Coupling Reaction:
[0093] The diene 27 was treated with the maleimido-derivatized
protein in water at 23.degree. C. Samples taken at various times
were diafiltered through a 10 kDa cutoff membrane then were
lyophilized and subjected to MALDI-TOF mass spectrometry. The
increase in molecular weight of the resulting conjugate 28
(n<29), relative to the mass of the core protein with the
dienophiles attached, is shown as a function of time in FIG. 1.
[0094] A similar time-dependent experiment was conducted with the
same maleimido-derivatized protein, using previously described
diene 8, and the results are shown in FIG. 2. Kinetic parameters
are shown in Table 2.
TABLE-US-00002 TABLE 2 Kinetic Parameters for Conjugation Reactions
Parameters for .DELTA.mw = .DELTA.mw.sub.max(1 c.sup.-kt) Diene
.DELTA.mw.sub.max K (min.sup.-1) t.sub.1/2 (min) 14 9000 0.007 99 8
4350 0.0045 154
Example 6
##STR00011##
[0096] Diene Component:
[0097] The previously described ester 16 was converted to hydrazide
17 by treatment with hydrazine in MeOH at 23.degree. C. for 24 hr.:
.sup.1H NMR (500 MHz, CD.sub.3OD): .delta.=6.00 (m, 2H), 5.57 (m,
1H), 5.49 (m, 1H), 2.33 (m, 2H), 2.21 (m, 2H), 1.70 (d, 3H).
[0098] Acylation with methyl malonyl chloride in pyridine at
-20.degree. C. for 20 min afforded the ester 29 in 72% yield,
.sup.1H NMR (500 MHz, CD.sub.3OD): .delta.=6.03 (m, 2H), 5.58 (m,
2H), 3.74 (s, 3H), 3.41 (m, 2H), 2.31-2.43 (m, 4H), 1.23 (d,
3H).
[0099] Hydrolysis with LiOH in methanol (23.degree. C., 1 hr),
followed by acidification with 1 N HCl, gave the crystalline acid
30 in 74% yield, .sup.1H NMR (500 MHz, CD.sub.3OD): .delta.=5.97
(m, 2H), 5.52 (m, 2H), 3.26 (m, 2H), 3.24-3.37 (m, 4H), 1.66 (d,
3H).
##STR00012##
[0100] Treatment of the dodecasaccharide hydrazide 31 (V. Pozsgay,
J. Org. Chem. 1998, 63:5983) with the linker 30 in DMF in the
presence of HATU led to the diene-equipped construct 32, which was
purified by gel-filtration through a Biogel P-4 column using water
as the eluant. .sup.1H NMR (500 MHz, D.sub.2O): .delta.=6.11 (m,
2H), 5.72 (m, 1H), 5.61 (m, 1H), 5.60 (br s, 3H), 5.11 (br, s 2H),
5.08 (br s, 1H), 5.06 (br s, 2H), 5.04 (d, 2H), 5.00 (d, 1H), 4.81
(br s, 1H), 1.71 (d, 3H); FAB-MS (dithiothreitol-dithioerythritol
positive ion) 2363.9 (M+Na), calcd.: 2362.9.
[0101] Dienophile Component:
[0102] A stirred solution of human serum albumin in pH 7.5 buffer
(1 ml) was treated at 5.degree. C. (ice-bath) with
3-sulfosuccinimidyl 4-maleimidobutyrate (1) as described above, to
provide maleimido-derivatized protein containing an average of 22
maleimido groups per molecule of protein.
[0103] Coupling Reaction:
[0104] Under conditions similar to those used for the conjugation
of constructs 8 and 27, cycloaddition of 32 onto the
maleimido-derivatized protein took place at a slower rate. After 2
hours, the average incorporation was 1.5 dodecasaccharide chains
per HSA molecule, and after 8 hours, the incorporation reached an
average of 3.
* * * * *