U.S. patent application number 14/021122 was filed with the patent office on 2014-10-30 for development of sensitive fret sensors and methods of using the same. This patent application is currently assigned to Carnegie Institution of Washington. The applicant listed for this patent is Carnegie Institution of Washington. Invention is credited to Marcus Fehr, Wolf B. Frommer, Loren Looger, Sakiko Okumoto.
Application Number | 20140323692 14/021122 |
Document ID | / |
Family ID | 36203532 |
Filed Date | 2014-10-30 |
United States Patent Application | 20140323692 |
Kind Code | A1 |
Frommer; Wolf B. ; et al. | October 30, 2014 |
Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.
Inventors: | Frommer; Wolf B.; (Washington, DC) ; Okumoto; Sakiko; (Washington, DC) ; Looger; Loren; (Washington, DC) ; Fehr; Marcus; (Washington, DC) | ||||||||||
Applicant: |
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Assignee: | Carnegie Institution of
Washington Washington DC |
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Family ID: | 36203532 | ||||||||||
Appl. No.: | 14/021122 | ||||||||||
Filed: | September 9, 2013 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
11665339 | Sep 29, 2009 | 8530633 | ||
PCT/US05/36957 | Oct 14, 2005 | |||
14021122 | ||||
60618179 | Oct 14, 2004 | |||
60643576 | Jan 14, 2005 | |||
60654447 | Feb 22, 2005 | |||
60658141 | Mar 4, 2005 | |||
Current U.S. Class: | 530/350 ; 435/254.11; 435/320.1; 435/325; 435/353; 435/366; 435/419; 536/23.4 |
Current CPC Class: | G01N 33/542 20130101; C07K 14/245 20130101; G01N 2800/2835 20130101; G01N 2800/2857 20130101; C12Q 1/6818 20130101; G01N 33/566 20130101; G01N 2800/2871 20130101; G01N 2800/2814 20130101 |
Class at Publication: | 530/350 ; 536/23.4; 435/320.1; 435/366; 435/353; 435/325; 435/254.11; 435/419 |
International Class: | G01N 33/566 20060101 G01N033/566 |
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 59 <210> SEQ ID NO 1 <211> LENGTH: 978 <212>
TYPE: DNA <213> ORGANISM: Escherichia coli K12 <220>
FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(978)
<400> SEQUENCE: 1 atg ata aca aca caa aca ctc aca acg ggt atc
cat gcg ttc tta acg 48 Met Ile Thr Thr Gln Thr Leu Thr Thr Gly Ile
His Ala Phe Leu Thr 1 5 10 15 cag aag ata aag gag ttg gat atg caa
tta cgt aaa cct gcc aca gca 96 Gln Lys Ile Lys Glu Leu Asp Met Gln
Leu Arg Lys Pro Ala Thr Ala 20 25 30 atc ctc gcc ctg gcg ctt tcc
gca gga ctg gca cag gca gat gac gcc 144 Ile Leu Ala Leu Ala Leu Ser
Ala Gly Leu Ala Gln Ala Asp Asp Ala 35 40 45 gcc ccg gca gcg ggc
agt act ctg gac aaa atc gcc aaa aac ggt gtg 192 Ala Pro Ala Ala Gly
Ser Thr Leu Asp Lys Ile Ala Lys Asn Gly Val 50 55 60 att gtc gtc
ggt cac cgt gaa tct tca gtg cct ttc tct tat tac gac 240 Ile Val Val
Gly His Arg Glu Ser Ser Val Pro Phe Ser Tyr Tyr Asp 65 70 75 80 aat
cag caa aaa gtg gtg ggt tac tcg cag gat tac tcc aac gcc att 288 Asn
Gln Gln Lys Val Val Gly Tyr Ser Gln Asp Tyr Ser Asn Ala Ile 85 90
95 gtt gaa gca gtg aaa aag aaa ctc aac aaa ccg gac ttg cag gta aaa
336 Val Glu Ala Val Lys Lys Lys Leu Asn Lys Pro Asp Leu Gln Val Lys
100 105 110 ctg att ccg att acc tca caa aac cgt att cca ctg ctg caa
aac ggc 384 Leu Ile Pro Ile Thr Ser Gln Asn Arg Ile Pro Leu Leu Gln
Asn Gly 115 120 125 act ttc gat ttt gaa tgt ggt tct acc acc aac aac
gtc gaa cgc caa 432 Thr Phe Asp Phe Glu Cys Gly Ser Thr Thr Asn Asn
Val Glu Arg Gln 130 135 140 aaa cag gcg gct ttc tct gac act att ttc
gtg gtc ggt acg cgc ctg 480 Lys Gln Ala Ala Phe Ser Asp Thr Ile Phe
Val Val Gly Thr Arg Leu 145 150 155 160 ttg acc aaa aag ggt ggc gat
atc aaa gat ttt gcc aac ctg aaa gac 528 Leu Thr Lys Lys Gly Gly Asp
Ile Lys Asp Phe Ala Asn Leu Lys Asp 165 170 175 aaa gcc gta gtc gtc
act tcc ggc act acc tct gaa gtt ttg ctc aac 576 Lys Ala Val Val Val
Thr Ser Gly Thr Thr Ser Glu Val Leu Leu Asn 180 185 190 aaa ctg aat
gaa gag caa aaa atg aat atg cgc atc atc agc gcc aaa 624 Lys Leu Asn
Glu Glu Gln Lys Met Asn Met Arg Ile Ile Ser Ala Lys 195 200 205 gat
cac ggt gac tct ttc cgc acc ctg gaa agc ggt cgt gcc gtt gcc 672 Asp
His Gly Asp Ser Phe Arg Thr Leu Glu Ser Gly Arg Ala Val Ala 210 215
220 ttt atg atg gat gac gct ctg ctg gcc ggt gaa cgt gcg aaa gcg aag
720 Phe Met Met Asp Asp Ala Leu Leu Ala Gly Glu Arg Ala Lys Ala Lys
225 230 235 240 aaa cca gac aac tgg gaa atc gtc ggc aag ccg cag tct
cag gag gcc 768 Lys Pro Asp Asn Trp Glu Ile Val Gly Lys Pro Gln Ser
Gln Glu Ala 245 250 255 tac ggt tgt atg ttg cgt aaa gat gat ccg cag
ttc aaa aag ctg atg 816 Tyr Gly Cys Met Leu Arg Lys Asp Asp Pro Gln
Phe Lys Lys Leu Met 260 265 270 gat gac acc atc gct cag gtg cag acc
tcc ggt gaa gcg gaa aaa tgg 864 Asp Asp Thr Ile Ala Gln Val Gln Thr
Ser Gly Glu Ala Glu Lys Trp 275 280 285 ttt gat aag tgg ttc aaa aat
cca att ccg ccg aaa aac ctg aac atg 912 Phe Asp Lys Trp Phe Lys Asn
Pro Ile Pro Pro Lys Asn Leu Asn Met 290 295 300 aat ttc gaa ctg tca
gac gaa atg aaa gca ctg ttc aaa gaa ccg aat 960 Asn Phe Glu Leu Ser
Asp Glu Met Lys Ala Leu Phe Lys Glu Pro Asn 305 310 315 320 gac aag
gca ctg aac taa 978 Asp Lys Ala Leu Asn 325 <210> SEQ ID NO 2
<211> LENGTH: 325 <212> TYPE: PRT <213> ORGANISM:
Escherichia coli K12 <400> SEQUENCE: 2 Met Ile Thr Thr Gln
Thr Leu Thr Thr Gly Ile His Ala Phe Leu Thr 1 5 10 15 Gln Lys Ile
Lys Glu Leu Asp Met Gln Leu Arg Lys Pro Ala Thr Ala 20 25 30 Ile
Leu Ala Leu Ala Leu Ser Ala Gly Leu Ala Gln Ala Asp Asp Ala 35 40
45 Ala Pro Ala Ala Gly Ser Thr Leu Asp Lys Ile Ala Lys Asn Gly Val
50 55 60 Ile Val Val Gly His Arg Glu Ser Ser Val Pro Phe Ser Tyr
Tyr Asp 65 70 75 80 Asn Gln Gln Lys Val Val Gly Tyr Ser Gln Asp Tyr
Ser Asn Ala Ile 85 90 95 Val Glu Ala Val Lys Lys Lys Leu Asn Lys
Pro Asp Leu Gln Val Lys 100 105 110 Leu Ile Pro Ile Thr Ser Gln Asn
Arg Ile Pro Leu Leu Gln Asn Gly 115 120 125 Thr Phe Asp Phe Glu Cys
Gly Ser Thr Thr Asn Asn Val Glu Arg Gln 130 135 140 Lys Gln Ala Ala
Phe Ser Asp Thr Ile Phe Val Val Gly Thr Arg Leu 145 150 155 160 Leu
Thr Lys Lys Gly Gly Asp Ile Lys Asp Phe Ala Asn Leu Lys Asp 165 170
175 Lys Ala Val Val Val Thr Ser Gly Thr Thr Ser Glu Val Leu Leu Asn
180 185 190 Lys Leu Asn Glu Glu Gln Lys Met Asn Met Arg Ile Ile Ser
Ala Lys 195 200 205 Asp His Gly Asp Ser Phe Arg Thr Leu Glu Ser Gly
Arg Ala Val Ala 210 215 220 Phe Met Met Asp Asp Ala Leu Leu Ala Gly
Glu Arg Ala Lys Ala Lys 225 230 235 240 Lys Pro Asp Asn Trp Glu Ile
Val Gly Lys Pro Gln Ser Gln Glu Ala 245 250 255 Tyr Gly Cys Met Leu
Arg Lys Asp Asp Pro Gln Phe Lys Lys Leu Met 260 265 270 Asp Asp Thr
Ile Ala Gln Val Gln Thr Ser Gly Glu Ala Glu Lys Trp 275 280 285 Phe
Asp Lys Trp Phe Lys Asn Pro Ile Pro Pro Lys Asn Leu Asn Met 290 295
300 Asn Phe Glu Leu Ser Asp Glu Met Lys Ala Leu Phe Lys Glu Pro Asn
305 310 315 320 Asp Lys Ala Leu Asn 325 <210> SEQ ID NO 3
<211> LENGTH: 810 <212> TYPE: DNA <213> ORGANISM:
Escherichia coli K12 <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(810) <400> SEQUENCE: 3 ctg gac
aaa atc gcc aaa aac ggt gtg att gtc gtc ggt cac cgt gaa 48 Leu Asp
Lys Ile Ala Lys Asn Gly Val Ile Val Val Gly His Arg Glu 1 5 10 15
tct tca gtg cct ttc tct tat tac gac aat cag caa aaa gtg gtg ggt 96
Ser Ser Val Pro Phe Ser Tyr Tyr Asp Asn Gln Gln Lys Val Val Gly 20
25 30 tac tcg cag gat tac tcc aac gcc att gtt gaa gca gtg aaa aag
aaa 144 Tyr Ser Gln Asp Tyr Ser Asn Ala Ile Val Glu Ala Val Lys Lys
Lys 35 40 45 ctc aac aaa ccg gac ttg cag gta aaa ctg att ccg att
acc tca caa 192 Leu Asn Lys Pro Asp Leu Gln Val Lys Leu Ile Pro Ile
Thr Ser Gln 50 55 60 aac cgt att cca ctg ctg caa aac ggc act ttc
gat ttt gaa tgt ggt 240 Asn Arg Ile Pro Leu Leu Gln Asn Gly Thr Phe
Asp Phe Glu Cys Gly 65 70 75 80 tct acc acc aac aac gtc gaa cgc caa
aaa cag gcg gct ttc tct gac 288 Ser Thr Thr Asn Asn Val Glu Arg Gln
Lys Gln Ala Ala Phe Ser Asp 85 90 95 act att ttc gtg gtc ggt acg
cgc ctg ttg acc aaa aag ggt ggc gat 336 Thr Ile Phe Val Val Gly Thr
Arg Leu Leu Thr Lys Lys Gly Gly Asp 100 105 110 atc aaa gat ttt gcc
aac ctg aaa gac aaa gcc gta gtc gtc act tcc 384 Ile Lys Asp Phe Ala
Asn Leu Lys Asp Lys Ala Val Val Val Thr Ser 115 120 125 ggc act acc
tct gaa gtt ttg ctc aac aaa ctg aat gaa gag caa aaa 432 Gly Thr Thr
Ser Glu Val Leu Leu Asn Lys Leu Asn Glu Glu Gln Lys 130 135 140 atg
aat atg cgc atc atc agc gcc aaa gat cac ggt gac tct ttc cgc 480 Met
Asn Met Arg Ile Ile Ser Ala Lys Asp His Gly Asp Ser Phe Arg 145 150
155 160 acc ctg gaa agc ggt cgt gcc gtt gcc ttt atg atg gat gac gct
ctg 528 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe Met Met Asp Asp Ala
Leu 165 170 175 ctg gcc ggt gaa cgt gcg aaa gcg aag aaa cca gac aac
tgg gaa atc 576 Leu Ala Gly Glu Arg Ala Lys Ala Lys Lys Pro Asp Asn
Trp Glu Ile 180 185 190 gtc ggc aag ccg cag tct cag gag gcc tac ggt
tgt atg ttg cgt aaa 624 Val Gly Lys Pro Gln Ser Gln Glu Ala Tyr Gly
Cys Met Leu Arg Lys 195 200 205 gat gat ccg cag ttc aaa aag ctg atg
gat gac acc atc gct cag gtg 672 Asp Asp Pro Gln Phe Lys Lys Leu Met
Asp Asp Thr Ile Ala Gln Val 210 215 220 cag acc tcc ggt gaa gcg gaa
aaa tgg ttt gat aag tgg ttc aaa aat 720 Gln Thr Ser Gly Glu Ala Glu
Lys Trp Phe Asp Lys Trp Phe Lys Asn 225 230 235 240 cca att ccg ccg
aaa aac ctg aac atg aat ttc gaa ctg tca gac gaa 768 Pro Ile Pro Pro
Lys Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu 245 250 255 atg aaa
gca ctg ttc aaa gaa ccg aat gac aag gca ctg aac 810 Met Lys Ala Leu
Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn 260 265 270 <210> SEQ
ID NO 4 <211> LENGTH: 270 <212> TYPE: PRT <213>
ORGANISM: Escherichia coli K12 <400> SEQUENCE: 4 Leu Asp Lys
Ile Ala Lys Asn Gly Val Ile Val Val Gly His Arg Glu 1 5 10 15 Ser
Ser Val Pro Phe Ser Tyr Tyr Asp Asn Gln Gln Lys Val Val Gly 20 25
30 Tyr Ser Gln Asp Tyr Ser Asn Ala Ile Val Glu Ala Val Lys Lys Lys
35 40 45 Leu Asn Lys Pro Asp Leu Gln Val Lys Leu Ile Pro Ile Thr
Ser Gln 50 55 60 Asn Arg Ile Pro Leu Leu Gln Asn Gly Thr Phe Asp
Phe Glu Cys Gly 65 70 75 80 Ser Thr Thr Asn Asn Val Glu Arg Gln Lys
Gln Ala Ala Phe Ser Asp 85 90 95 Thr Ile Phe Val Val Gly Thr Arg
Leu Leu Thr Lys Lys Gly Gly Asp 100 105 110 Ile Lys Asp Phe Ala Asn
Leu Lys Asp Lys Ala Val Val Val Thr Ser 115 120 125 Gly Thr Thr Ser
Glu Val Leu Leu Asn Lys Leu Asn Glu Glu Gln Lys 130 135 140 Met Asn
Met Arg Ile Ile Ser Ala Lys Asp His Gly Asp Ser Phe Arg 145 150 155
160 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe Met Met Asp Asp Ala Leu
165 170 175 Leu Ala Gly Glu Arg Ala Lys Ala Lys Lys Pro Asp Asn Trp
Glu Ile 180 185 190 Val Gly Lys Pro Gln Ser Gln Glu Ala Tyr Gly Cys
Met Leu Arg Lys 195 200 205 Asp Asp Pro Gln Phe Lys Lys Leu Met Asp
Asp Thr Ile Ala Gln Val 210 215 220 Gln Thr Ser Gly Glu Ala Glu Lys
Trp Phe Asp Lys Trp Phe Lys Asn 225 230 235 240 Pro Ile Pro Pro Lys
Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu 245 250 255 Met Lys Ala
Leu Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn 260 265 270 <210>
SEQ ID NO 5 <211> LENGTH: 39 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer <400> SEQUENCE: 5
ggtaccggag gcgccgcagg cagcacgctg gacaaaatc 39 <210> SEQ ID NO
6 <211> LENGTH: 39 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer <400> SEQUENCE: 6 accggtaccg
gcgccgttca gtgccttgtc attcggttc 39 <210> SEQ ID NO 7
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer <400> SEQUENCE: 7 gagcccggga tggtgagcaa
gggcgaggag 30 <210> SEQ ID NO 8 <211> LENGTH: 33
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer
<400> SEQUENCE: 8 gaggtcgacc ttgtacagct cgtccatgcc gag 33
<210> SEQ ID NO 9 <211> LENGTH: 5206 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: DNA sequence of pRSETB FLIP-E 600n
vector <400> SEQUENCE: 9 atctcgatcc cgcgaaatta atacgactca
ctatagggag accacaacgg tttccctcta 60 gataattttg tttaacttta
agaaggagat atacatatgc ggggttctca tcatcatcat 120 catcatggta
tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat 180
gacgataagg atccgggccg catggtgagc aagggcgagg agctgttcac cggggtggtg
240 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
gtccggcgag 300 ggcgagggcg atgccaccta cggcaagctg accctgaagt
tcatctgcac caccggcaag 360 ctgcccgtgc cctggcccac cctcgtgacc
accctgacct ggggcgtgca gtgcttcagc 420 cgctaccccg accacatgaa
gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 480 gtccaggagc
gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 540
aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
600 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
cgtctatatc 660 accgccgaca agcagaagaa cggcatcaag gccaacttca
agatccgcca caacatcgag 720 gacggcagcg tgcagctcgc cgaccactac
cagcagaaca cccccatcgg cgacggcccc 780 gtgctgctgc ccgacaacca
ctacctgagc acccagtccg ccctgagcaa agaccccaac 840 gagaagcgcg
atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 900
atggacgagc tgtacaaggg tggtaccgga ggcgccgcag gcagcacgct ggacaaaatc
960 gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt cagtgccttt
ctcttattac 1020 gacaatcagc aaaaagtggt gggttactcg caggattact
ccaacgccat tgttgaagca 1080 gtgaaaaaga aactcaacaa accggacttg
caggtaaaac tgattccgat tacctcacaa 1140 aaccgtattc cactgctgca
aaacggcact ttcgattttg aatgtggttc taccaccaac 1200 aacgtcgaac
gccaaaaaca ggcggctttc tctgacacta ttttcgtggt cggtacgcgc 1260
ctgttgacca aaaagggtgg cgatatcaaa gattttgcca acctgaaaga caaagccgta
1320 gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca aactgaatga
agagcaaaaa 1380 atgaatatgc gcatcatcag cgccaaagat cacggtgact
ctttccgcac cctggaaagc 1440 ggtcgtgccg ttgcctttat gatggatgac
gctctgctgg ccggtgaacg tgcgaaagcg 1500 aagaaaccag acaactggga
aatcgtcggc aagccgcagt ctcaggaggc ctacggttgt 1560 atgttgcgta
aagatgatcc gcagttcaaa aagctgatgg atgacaccat cgctcaggtg 1620
cagacctccg gtgaagcgga aaaatggttt gataagtggt tcaaaaatcc aattccgccg
1680 aaaaacctga acatgaattt cgaactgtca gacgaaatga aagcactgtt
caaagaaccg 1740 aatgacaagg cactgaacgg cgccggtacc ggtggaatgg
tgagcaaggg cgaggagctg 1800 ttcaccgggg tggtgcccat cctggtcgag
ctggacggcg acgtaaacgg ccacaagttc 1860 agcgtgtccg gcgagggcga
gggcgatgcc acctacggca agctgaccct gaagttcatc 1920 tgcaccaccg
gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc 1980
ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
2040 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
caactacaag 2100 acccgcgccg aggtgaagtt cgagggcgac accctggtga
accgcatcga gctgaagggc 2160 atcgacttca aggaggacgg caacatcctg
gggcacaagc tggagtacaa ctacaacagc 2220 cacaacgtct atatcatggc
cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 2280 cgccacaaca
tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2340
atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
2400 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
gaccgccgcc 2460 gggatcactc tcggcatgga cgagctgtac aagtaaaagc
ttgatccggc tgctaacaaa 2520 gcccgaaagg aagctgagtt ggctgctgcc
accgctgagc aataactagc ataacccctt 2580 ggggcctcta aacgggtctt
gaggggtttt ttgctgaaag gaggaactat atccggatct 2640 ggcgtaatag
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 2700
gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
2760 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
ttcccttcct 2820 ttctcgccac gttcgccggc tttccccgtc aagctctaaa
tcgggggctc cctttagggt 2880 tccgatttag agctttacgg cacctcgacc
gcaaaaaact tgatttgggt gatggttcac 2940 gtagtgggcc atcgccctga
tagacggttt ttcgcccttt gacgttggag tccacgttct 3000 ttaatagtgg
actcttgttc caaactggaa caacactcaa ccctatcgcg gtctattctt 3060
ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3120 aaatatttaa cgcgaatttt aacaaaatat taacgtttac aatttcgcct
gatgcggtat 3180 tttctcctta cgcatctgtg cggtatttca caccgcatac
aggtggcact tttcggggaa 3240 atgtgcgcgg aacccctatt tgtttatttt
tctaaataca ttcaaatatg tatccgctca 3300 tgagacaata accctgataa
atgcttcaat aatattgaaa aaggaagagt atgagtattc 3360 aacatttccg
tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 3420
acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
3480 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
gaagaacgtt 3540 ttccaatgat gagcactttt aaagttctgc tatgtgatac
actattatcc cgtattgacg 3600 ccgggcaaga gcaactcggt cgccgcatac
actattctca gaatgacttg gttgagtact 3660 caccagtcac agaaaagcat
cttacggatg gcatgacagt aagagaatta tgcagtgctg 3720 ccataaccat
gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 3780
aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
3840 aaccggagct gaatgaagcc ataccaaacg acgagagtga caccacgatg
cctgtagcaa 3900 tgccaacaac gttgcgcaaa ctattaactg gcgaactact
tactctagct tcccggcaac 3960 aattaataga ctgaatggag gcggataaag
ttgcaggacc acttctgcgc tcggcccttc 4020 cggctggctg gtttattgct
gataaatctg gagccggtga gcgtgggtct cgcggtatca 4080 ttgcagcact
ggggccagat ggtaagcgct cccgtatcgt agttatctac acgacgggga 4140
gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
4200 agcattggta actgtcagac caagtttact catatatact ttagattgat
ttaaaacttc 4260 atttttaatt taaaaggatc taggtgaaga tcctttttga
taatctcatg accaaaatcc 4320 cttaacgtga gttttcgttc cactgagcgt
cagaccccgt agaaaagatc aaaggatctt 4380 cttgagatcc tttttttctg
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4440 cagcggtggt
ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4500
tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
4560 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
ccagtggctg 4620 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
aagacgatag ttaccggata 4680 aggcgcagcg gtcgggctga acggggggtt
cgtgcacaca gcccagcttg gagcgaacga 4740 cctacaccga actgagatac
ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4800 ggagaaaggc
ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4860
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
4920 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
aacgccagca 4980 acgcggcctt tttacggttc ctgggctttt gctggccttt
tgctcacatg ttctttcctg 5040 cgttatcccc tgattctgtg gataaccgta
ttaccgcctt tgagtgagct gataccgctc 5100 gccgcagccg aacgaccgag
cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 5160 tacgcaaacc
gcctctcccc gcgcgttggc cgattcatta atgcag 5206 <210> SEQ ID NO
10 <211> LENGTH: 5206 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: DNA sequence of pRSETB FLIP-E 10u vector
<400> SEQUENCE: 10 atctcgatcc cgcgaaatta atacgactca
ctatagggag accacaacgg tttccctcta 60 gataattttg tttaacttta
agaaggagat atacatatgc ggggttctca tcatcatcat 120 catcatggta
tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat 180
gacgataagg atccgggccg catggtgagc aagggcgagg agctgttcac cggggtggtg
240 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
gtccggcgag 300 ggcgagggcg atgccaccta cggcaagctg accctgaagt
tcatctgcac caccggcaag 360 ctgcccgtgc cctggcccac cctcgtgacc
accctgacct ggggcgtgca gtgcttcagc 420 cgctaccccg accacatgaa
gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 480 gtccaggagc
gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 540
aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
600 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
cgtctatatc 660 accgccgaca agcagaagaa cggcatcaag gccaacttca
agatccgcca caacatcgag 720 gacggcagcg tgcagctcgc cgaccactac
cagcagaaca cccccatcgg cgacggcccc 780 gtgctgctgc ccgacaacca
ctacctgagc acccagtccg ccctgagcaa agaccccaac 840 gagaagcgcg
atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 900
atggacgagc tgtacaaggg tggtaccgga ggcgccgcag gcagcacgct ggacaaaatc
960 gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt cagtgccttt
ctcttattac 1020 gacaatcagc aaaaagtggt gggttactcg caggattact
ccaacgccat tgttgaagca 1080 gtgaaaaaga aactcaacaa accggacttg
caggtaaaac tgattccgat tacctcacaa 1140 aaccgtattc cactgctgca
aaacggcact ttcgattttg aatgtggttc taccaccaac 1200 aacgtcgaac
gccaaaaaca ggcggctttc tctgacacta ttttcgtggt cggtacgcgc 1260
ctgttgacca aaaagggtgg cgatatcaaa gattttgcca acctgaaaga caaagccgta
1320 gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca aactgaatga
agagcaaaaa 1380 atgaatatgc gcatcatcag cgccaaagat cacggtgact
ctttccgcac cctggaaagc 1440 ggtcgtgccg ttgcctttat gatggatgac
cggctgctgg ccggtgaacg tgcgaaagcg 1500 aagaaaccag acaactggga
aatcgtcggc aagccgcagt ctcaggaggc ctacggttgt 1560 atgttgcgta
aagatgatcc gcagttcaaa aagctgatgg atgacaccat cgctcaggtg 1620
cagacctccg gtgaagcgga aaaatggttt gataagtggt tcaaaaatcc aattccgccg
1680 aaaaacctga acatgaattt cgaactgtca gacgaaatga aagcactgtt
caaagaaccg 1740 aatgacaagg cactgaacgg cgccggtacc ggtggaatgg
tgagcaaggg cgaggagctg 1800 ttcaccgggg tggtgcccat cctggtcgag
ctggacggcg acgtaaacgg ccacaagttc 1860 agcgtgtccg gcgagggcga
gggcgatgcc acctacggca agctgaccct gaagttcatc 1920 tgcaccaccg
gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc 1980
ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
2040 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
caactacaag 2100 acccgcgccg aggtgaagtt cgagggcgac accctggtga
accgcatcga gctgaagggc 2160 atcgacttca aggaggacgg caacatcctg
gggcacaagc tggagtacaa ctacaacagc 2220 cacaacgtct atatcatggc
cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 2280 cgccacaaca
tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2340
atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
2400 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
gaccgccgcc 2460 gggatcactc tcggcatgga cgagctgtac aagtaaaagc
ttgatccggc tgctaacaaa 2520 gcccgaaagg aagctgagtt ggctgctgcc
accgctgagc aataactagc ataacccctt 2580 ggggcctcta aacgggtctt
gaggggtttt ttgctgaaag gaggaactat atccggatct 2640 ggcgtaatag
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 2700
gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
2760 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
ttcccttcct 2820 ttctcgccac gttcgccggc tttccccgtc aagctctaaa
tcgggggctc cctttagggt 2880 tccgatttag agctttacgg cacctcgacc
gcaaaaaact tgatttgggt gatggttcac 2940 gtagtgggcc atcgccctga
tagacggttt ttcgcccttt gacgttggag tccacgttct 3000 ttaatagtgg
actcttgttc caaactggaa caacactcaa ccctatcgcg gtctattctt 3060
ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3120 aaatatttaa cgcgaatttt aacaaaatat taacgtttac aatttcgcct
gatgcggtat 3180 tttctcctta cgcatctgtg cggtatttca caccgcatac
aggtggcact tttcggggaa 3240 atgtgcgcgg aacccctatt tgtttatttt
tctaaataca ttcaaatatg tatccgctca 3300 tgagacaata accctgataa
atgcttcaat aatattgaaa aaggaagagt atgagtattc 3360 aacatttccg
tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 3420
acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
3480 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
gaagaacgtt 3540 ttccaatgat gagcactttt aaagttctgc tatgtgatac
actattatcc cgtattgacg 3600 ccgggcaaga gcaactcggt cgccgcatac
actattctca gaatgacttg gttgagtact 3660 caccagtcac agaaaagcat
cttacggatg gcatgacagt aagagaatta tgcagtgctg 3720 ccataaccat
gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 3780
aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
3840 aaccggagct gaatgaagcc ataccaaacg acgagagtga caccacgatg
cctgtagcaa 3900 tgccaacaac gttgcgcaaa ctattaactg gcgaactact
tactctagct tcccggcaac 3960 aattaataga ctgaatggag gcggataaag
ttgcaggacc acttctgcgc tcggcccttc 4020 cggctggctg gtttattgct
gataaatctg gagccggtga gcgtgggtct cgcggtatca 4080 ttgcagcact
ggggccagat ggtaagcgct cccgtatcgt agttatctac acgacgggga 4140
gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
4200 agcattggta actgtcagac caagtttact catatatact ttagattgat
ttaaaacttc 4260 atttttaatt taaaaggatc taggtgaaga tcctttttga
taatctcatg accaaaatcc 4320 cttaacgtga gttttcgttc cactgagcgt
cagaccccgt agaaaagatc aaaggatctt 4380 cttgagatcc tttttttctg
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4440 cagcggtggt
ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4500
tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
4560 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
ccagtggctg 4620 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
aagacgatag ttaccggata 4680 aggcgcagcg gtcgggctga acggggggtt
cgtgcacaca gcccagcttg gagcgaacga 4740 cctacaccga actgagatac
ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4800 ggagaaaggc
ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4860
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
4920 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
aacgccagca 4980 acgcggcctt tttacggttc ctgggctttt gctggccttt
tgctcacatg ttctttcctg 5040 cgttatcccc tgattctgtg gataaccgta
ttaccgcctt tgagtgagct gataccgctc 5100 gccgcagccg aacgaccgag
cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 5160 tacgcaaacc
gcctctcccc gcgcgttggc cgattcatta atgcag 5206 <210> SEQ ID NO
11 <211> LENGTH: 7603 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: DNA sequence of pDisplay FLIP-E 600n <400>
SEQUENCE: 11 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg
gggtcattag 60 ttcatagccc atatatggag ttccgcgtta cataacttac
ggtaaatggc ccgcctggct 120 gaccgcccaa cgacccccgc ccattgacgt
caataatgac gtatgttccc atagtaacgc 180 caatagggac tttccattga
cgtcaatggg tggactattt acggtaaact gcccacttgg 240 cagtacatca
agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 300
ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
360 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
atcaatgggc 420 gtggatagcg gtttgactca cggggatttc caagtctcca
ccccattgac gtcaatggga 480 gtttgttttg gcaccaaaat caacgggact
ttccaaaatg tcgtaacaac tccgccccat 540 tgacgcaaat gggcggtagg
cgtgtacggt gggaggtcta tataagcaga gctctctggc 600 taactagaga
acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 660
cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
720 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc
tgctctgggt 780 tccaggttcc actggtgact atccatatga tgttccagat
tatgctgggg cccagccggc 840 cagatctccc gggatggtga gcaagggcga
ggagctgttc accggggtgg tgcccatcct 900 ggtcgagctg gacggcgacg
taaacggcca caagttcagc gtgtccggcg agggcgaggg 960 cgatgccacc
tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 1020
gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
1080 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
acgtccagga 1140 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
cgcgccgagg tgaagttcga 1200 gggcgacacc ctggtgaacc gcatcgagct
gaagggcatc gacttcaagg aggacggcaa 1260 catcctgggg cacaagctgg
agtacaacta catcagccac aacgtctata tcaccgccga 1320 caagcagaag
aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag 1380
cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
1440 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
acgagaagcg 1500 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
atcactctcg gcatggacga 1560 gctgtacaag ggtggtaccg gaggcgccgc
aggcagcacg ctggacaaaa tcgccaaaaa 1620 cggtgtgatt gtcgtcggtc
accgtgaatc ttcagtgcct ttctcttatt acgacaatca 1680 gcaaaaagtg
gtgggttact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa 1740
gaaactcaac aaaccggact tgcaggtaaa actgattccg attacctcac aaaaccgtat
1800 tccactgctg caaaacggca ctttcgattt tgaatgtggt tctaccacca
acaacgtcga 1860 acgccaaaaa caggcggctt tctctgacac tattttcgtg
gtcggtacgc gcctgttgac 1920 caaaaagggt ggcgatatca aagattttgc
caacctgaaa gacaaagccg tagtcgtcac 1980 ttccggcact acctctgaag
ttttgctcaa caaactgaat gaagagcaaa aaatgaatat 2040 gcgcatcatc
agcgccaaag atcacggtga ctctttccgc accctggaaa gcggtcgtgc 2100
cgttgccttt atgatggatg acgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc
2160 agacaactgg gaaatcgtcg gcaagccgca gtctcaggag gcctacggtt
gtatgttgcg 2220 taaagatgat ccgcagttca aaaagctgat ggatgacacc
atcgctcagg tgcagacctc 2280 cggtgaagcg gaaaaatggt ttgataagtg
gttcaaaaat ccaattccgc cgaaaaacct 2340 gaacatgaat ttcgaactgt
cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa 2400 ggcactgaac
ggcgccggta ccggtggaat ggtgagcaag ggcgaggagc tgttcaccgg 2460
ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
2520 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
tctgcaccac 2580 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
ttcggctacg gcctgcagtg 2640 cttcgcccgc taccccgacc acatgaagca
gcacgacttc ttcaagtccg ccatgcccga 2700 aggctacgtc caggagcgca
ccatcttctt caaggacgac ggcaactaca agacccgcgc 2760 cgaggtgaag
ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 2820
caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
2880 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
tccgccacaa 2940 catcgaggac ggcagcgtgc agctcgccga ccactaccag
cagaacaccc ccatcggcga 3000 cggccccgtg ctgctgcccg acaaccacta
cctgagctac cagtccgccc tgagcaaaga 3060 ccccaacgag aagcgcgatc
acatggtcct gctggagttc gtgaccgccg ccgggatcac 3120 tctcggcatg
gacgagctgt acaaggtcga cgaacaaaaa ctcatctcag aagaggatct 3180
gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa
3240 ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct
cccttatcat 3300 cctcatcatg ctttggcaga agaagccacg ttaggcggcc
gctcgagatc agcctcgact 3360 gtgccttcta gttgccagcc atctgttgtt
tgcccctccc ccgtgccttc cttgaccctg 3420 gaaggtgcca ctcccactgt
cctttcctaa taaaatgagg aaattgcatc gcattgtctg 3480 agtaggtgtc
attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 3540
gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
3600 accagtggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
agaacatgtg 3660 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
gcgttgctgg cgtttttcca 3720 taggctccgc ccccctgacg agcatcacaa
aaatcgacgc tcaagtcaga ggtggcgaaa 3780 cccgacagga ctataaagat
accaggcgtt tccccctgga agctccctcg tgcgctctcc 3840 tgttccgacc
ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3900
gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
3960 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
gtaactatcg 4020 tcttgagtcc aacccggtaa gacacgactt atcgccactg
gcagcagcca ctggtaacag 4080 gattagcaga gcgaggtatg taggcggtgc
tacagagttc ttgaagtggt ggcctaacta 4140 cggctacact agaaggacag
tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4200 aaaaagagtt
ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4260
tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
4320 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
tggtcatgag 4380 attatcaaaa aggatcttca cctagatcct tttaaattaa
aaatgaagtt ttaaatcaat 4440 ctaaagtata tatgagtaac ctgaggctat
ggcagggcct gccgccccga cgttggctgc 4500 gagccctggg ccttcacccg
aacttggggg gtggggtggg gaaaaggaag aaacgcgggc 4560 gtattggccc
caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac 4620
cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
4680 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
tccccctagg 4740 gtgggcgaag aactccagca tgagatcccc gcgctggagg
atcatccagc cggcgtcccg 4800 gaaaacgatt ccgaagccca acctttcata
gaaggcggcg gtggaatcga aatctcgtga 4860 tggcaggttg ggcgtcgctt
ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac 4920 tcgtcaagaa
ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc 4980
acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
5040 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
tccagaaaag 5100 cggccatttt ccaccatgat attcggcaag caggcatcgc
catgggtcac gacgagatcc 5160 tcgccgtcgg gcatgctcgc cttgagcctg
gcgaacagtt cggctggcgc gagcccctga 5220 tgctcttgat catcctgatc
gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg 5280 cgatgtttcg
cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc 5340
attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
5400 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
aacgtcgagc 5460 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
gccgcgctgc ctcgtcttgc 5520 agttcattca gggcaccgga caggtcggtc
ttgacaaaaa gaaccgggcg cccctgcgct 5580 gacagccgga acacggcggc
atcagagcag ccgattgtct gttgtgccca gtcatagccg 5640 aatagcctct
ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg 5700
cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
5760 gcctcctcac tacttctgga atagctcaga ggccgaggag gcggcctcgg
cctctgcata 5820 aataaaaaaa attagtcagc catggggcgg agaatgggcg
gaactgggcg gagttagggg 5880 cgggatgggc ggagttaggg gcgggactat
ggttgctgac taattgagat gcatgctttg 5940 catacttctg cctgctgggg
agcctgggga ctttccacac ctggttgctg actaattgag 6000 atgcatgctt
tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg 6060
acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca ataaatcaat
6120 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
gtgaggcacc 6180 tatctcagcg atctgtctat ttcgttcatc catagttgcc
tgactccccg tcgtgtagat 6240 aactacgata cgggagggct taccatctgg
ccccagtgct gcaatgatac cgcgagaccc 6300 acgctcaccg gctccagatt
tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6360 aagtggtcct
gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 6420
agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
6480 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
gatcaaggcg 6540 agttacatga tcccccatgt tgtgcaaaaa agcggttagc
tccttcggtc ctccgatcgt 6600 tgtcagaagt aagttggccg cagtgttatc
actcatggtt atggcagcac tgcataattc 6660 tcttactgtc atgccatccg
taagatgctt ttctgtgact ggtgagtact caaccaagtc 6720 attctgagaa
tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6780
taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
6840 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
ctcgtgcacc 6900 caactgatct tcagcatctt ttactttcac cagcgtttct
gggtgagcaa aaacaggaag 6960 gcaaaatgcc gcaaaaaagg gaataagggc
gacacggaaa tgttgaatac tcatactctt 7020 cctttttcaa tattattgaa
gcatttatca gggttattgt ctcatgagcg gatacatatt 7080 tgaatgtatt
tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 7140
acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
7200 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
cttcctttct 7260 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
gggctccctt tagggttccg 7320 atttagtgct ttacggcacc tcgaccccaa
aaaacttgat tagggtgatg gttcacgtag 7380 tgggccatcg ccctgataga
cggtttttcg ccctttgacg ttggagtcca cgttctttaa 7440 tagtggactc
ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 7500
tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
7560 atttaacgcg aattttaaca aaatattaac gcttacaatt tac 7603
<210> SEQ ID NO 12 <211> LENGTH: 7603 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: DNA sequence of pDisplay FLIP-E 10u
<400> SEQUENCE: 12 gcgcgcgttg acattgatta ttgactagtt
attaatagta atcaattacg gggtcattag 60 ttcatagccc atatatggag
ttccgcgtta cataacttac ggtaaatggc ccgcctggct 120 gaccgcccaa
cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 180
caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
240 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
gacggtaaat 300 ggcccgcctg gcattatgcc cagtacatga ccttatggga
ctttcctact tggcagtaca 360 tctacgtatt agtcatcgct attaccatgg
tgatgcggtt ttggcagtac atcaatgggc 420 gtggatagcg gtttgactca
cggggatttc caagtctcca ccccattgac gtcaatggga 480 gtttgttttg
gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 540
tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
600 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
tatagggaga 660 cccaagcttg gtaccgagct cggatccact agtaacggcc
gccagtgtgc tggaattcgg 720 cttggggata tccaccatgg agacagacac
actcctgcta tgggtactgc tgctctgggt 780 tccaggttcc actggtgact
atccatatga tgttccagat tatgctgggg cccagccggc 840 cagatctccc
gggatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 900
ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
960 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
agctgcccgt 1020 gccctggccc accctcgtga ccaccctgac ctggggcgtg
cagtgcttca gccgctaccc 1080 cgaccacatg aagcagcacg acttcttcaa
gtccgccatg cccgaaggct acgtccagga 1140 gcgcaccatc ttcttcaagg
acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 1200 gggcgacacc
ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 1260
catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
1320 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
aggacggcag 1380 cgtgcagctc gccgaccact accagcagaa cacccccatc
ggcgacggcc ccgtgctgct 1440 gcccgacaac cactacctga gcacccagtc
cgccctgagc aaagacccca acgagaagcg 1500 cgatcacatg gtcctgctgg
agttcgtgac cgccgccggg atcactctcg gcatggacga 1560 gctgtacaag
ggtggtaccg gaggcgccgc aggcagcacg ctggacaaaa tcgccaaaaa 1620
cggtgtgatt gtcgtcggtc accgtgaatc ttcagtgcct ttctcttatt acgacaatca
1680 gcaaaaagtg gtgggttact cgcaggatta ctccaacgcc attgttgaag
cagtgaaaaa 1740 gaaactcaac aaaccggact tgcaggtaaa actgattccg
attacctcac aaaaccgtat 1800 tccactgctg caaaacggca ctttcgattt
tgaatgtggt tctaccacca acaacgtcga 1860 acgccaaaaa caggcggctt
tctctgacac tattttcgtg gtcggtacgc gcctgttgac 1920 caaaaagggt
ggcgatatca aagattttgc caacctgaaa gacaaagccg tagtcgtcac 1980
ttccggcact acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat
2040 gcgcatcatc agcgccaaag atcacggtga ctctttccgc accctggaaa
gcggtcgtgc 2100 cgttgccttt atgatggatg accggctgct ggccggtgaa
cgtgcgaaag cgaagaaacc 2160 agacaactgg gaaatcgtcg gcaagccgca
gtctcaggag gcctacggtt gtatgttgcg 2220 taaagatgat ccgcagttca
aaaagctgat ggatgacacc atcgctcagg tgcagacctc 2280 cggtgaagcg
gaaaaatggt ttgataagtg gttcaaaaat ccaattccgc cgaaaaacct 2340
gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa
2400 ggcactgaac ggcgccggta ccggtggaat ggtgagcaag ggcgaggagc
tgttcaccgg 2460 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
ggccacaagt tcagcgtgtc 2520 cggcgagggc gagggcgatg ccacctacgg
caagctgacc ctgaagttca tctgcaccac 2580 cggcaagctg cccgtgccct
ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg 2640 cttcgcccgc
taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 2700
aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
2760 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
gcatcgactt 2820 caaggaggac ggcaacatcc tggggcacaa gctggagtac
aactacaaca gccacaacgt 2880 ctatatcatg gccgacaagc agaagaacgg
catcaaggtg aacttcaaga tccgccacaa 2940 catcgaggac ggcagcgtgc
agctcgccga ccactaccag cagaacaccc ccatcggcga 3000 cggccccgtg
ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga 3060
ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
3120 tctcggcatg gacgagctgt acaaggtcga cgaacaaaaa ctcatctcag
aagaggatct 3180 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg
ccacactcct tgccctttaa 3240 ggtggtggtg atctcagcca tcctggccct
ggtggtgctc accatcatct cccttatcat 3300 cctcatcatg ctttggcaga
agaagccacg ttaggcggcc gctcgagatc agcctcgact 3360 gtgccttcta
gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 3420
gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
3480 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
ggaggattgg 3540 gaagacaata gcaggcatgc tggggatgcg gtgggctcta
tggcttctga ggcggaaaga 3600 accagtggcg gtaatacggt tatccacaga
atcaggggat aacgcaggaa agaacatgtg 3660 agcaaaaggc cagcaaaagg
ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3720 taggctccgc
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3780
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
3840 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
gaagcgtggc 3900 gctttctcat agctcacgct gtaggtatct cagttcggtg
taggtcgttc gctccaagct 3960 gggctgtgtg cacgaacccc ccgttcagcc
cgaccgctgc gccttatccg gtaactatcg 4020 tcttgagtcc aacccggtaa
gacacgactt atcgccactg gcagcagcca ctggtaacag 4080 gattagcaga
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4140
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
4200 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
gtggtttttt 4260 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
caagaagatc ctttgatctt 4320 ttctacgggg tctgacgctc agtggaacga
aaactcacgt taagggattt tggtcatgag 4380 attatcaaaa aggatcttca
cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4440 ctaaagtata
tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc 4500
gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
4560 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct
gggaccgaac 4620 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
ttctgtcttt ttattgccgt 4680 catagcgcgg gttccttccg gtattgtctc
cttccgtgtt tcagttagcc tccccctagg 4740 gtgggcgaag aactccagca
tgagatcccc gcgctggagg atcatccagc cggcgtcccg 4800 gaaaacgatt
ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga 4860
tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
4920 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
accgtaaagc 4980 acgaggaagc ggtcagccca ttcgccgcca agctcttcag
caatatcacg ggtagccaac 5040 gctatgtcct gatagcggtc cgccacaccc
agccggccac agtcgatgaa tccagaaaag 5100 cggccatttt ccaccatgat
attcggcaag caggcatcgc catgggtcac gacgagatcc 5160 tcgccgtcgg
gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga 5220
tgctcttgat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
5280 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
cagccgccgc 5340 attgcatcag ccatgatgga tactttctcg gcaggagcaa
ggtgagatga caggagatcc 5400 tgccccggca cttcgcccaa tagcagccag
tcccttcccg cttcagtgac aacgtcgagc 5460 acagctgcgc aaggaacgcc
cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc 5520 agttcattca
gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 5580
gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
5640 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
ttcaatcatg 5700 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa
aagcctaggc ctccaaaaaa 5760 gcctcctcac tacttctgga atagctcaga
ggccgaggag gcggcctcgg cctctgcata 5820 aataaaaaaa attagtcagc
catggggcgg agaatgggcg gaactgggcg gagttagggg 5880 cgggatgggc
ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg 5940
catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
6000 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
accctaactg 6060 acacacattc cacagctggt tctttccgcc tcaggactct
tcctttttca ataaatcaat 6120 ctaaagtata tatgagtaaa cttggtctga
cagttaccaa tgcttaatca gtgaggcacc 6180 tatctcagcg atctgtctat
ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6240 aactacgata
cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 6300
acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
6360 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
gggaagctag 6420 agtaagtagt tcgccagtta atagtttgcg caacgttgtt
gccattgcta caggcatcgt 6480 ggtgtcacgc tcgtcgtttg gtatggcttc
attcagctcc ggttcccaac gatcaaggcg 6540 agttacatga tcccccatgt
tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6600 tgtcagaagt
aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6660
tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
6720 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
tacgggataa 6780 taccgcgcca catagcagaa ctttaaaagt gctcatcatt
ggaaaacgtt cttcggggcg 6840 aaaactctca aggatcttac cgctgttgag
atccagttcg atgtaaccca ctcgtgcacc 6900 caactgatct tcagcatctt
ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6960 gcaaaatgcc
gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7020
cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
7080 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
gaaaagtgcc 7140 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
gtggtggtta cgcgcagcgt 7200 gaccgctaca cttgccagcg ccctagcgcc
cgctcctttc gctttcttcc cttcctttct 7260 cgccacgttc gccggctttc
cccgtcaagc tctaaatcgg gggctccctt tagggttccg 7320 atttagtgct
ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 7380
tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
7440 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
attcttttga 7500 tttataaggg attttgccga tttcggccta ttggttaaaa
aatgagctga tttaacaaaa 7560 atttaacgcg aattttaaca aaatattaac
gcttacaatt tac 7603 <210> SEQ ID NO 13 <211> LENGTH:
799 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Amino acid
sequence of FLIP-E 600n (expressed as 6xHis fusion in pRSET FLIP-E
600n) <400> SEQUENCE: 13 Met Arg Gly Ser His His His His His
His Gly Met Ala Ser Met Thr 1 5 10 15 Gly Gly Gln Gln Met Gly Arg
Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 Pro Gly Arg Met Val
Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40 45 Pro Ile Leu
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 50 55 60 Val
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 65 70
75 80 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr
Leu 85 90 95 Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg
Tyr Pro Asp 100 105 110 His Met Lys Gln His Asp Phe Phe Lys Ser Ala
Met Pro Glu Gly Tyr 115 120 125 Val Gln Glu Arg Thr Ile Phe Phe Lys
Asp Asp Gly Asn Tyr Lys Thr 130 135 140 Arg Ala Glu Val Lys Phe Glu
Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 Leu Lys Gly Ile
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170 175 Leu Glu
Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys 180 185 190
Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu 195
200 205 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro
Ile 210 215 220 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu
Ser Thr Gln 225 230 235 240 Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys
Arg Asp His Met Val Leu 245 250 255 Leu Glu Phe Val Thr Ala Ala Gly
Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 Tyr Lys Gly Gly Thr Gly
Gly Ala Ala Gly Ser Thr Leu Asp Lys Ile 275 280 285 Ala Lys Asn Gly
Val Ile Val Val Gly His Arg Glu Ser Ser Val Pro 290 295 300 Phe Ser
Tyr Tyr Asp Asn Gln Gln Lys Val Val Gly Tyr Ser Gln Asp 305 310 315
320 Tyr Ser Asn Ala Ile Val Glu Ala Val Lys Lys Lys Leu Asn Lys Pro
325 330 335 Asp Leu Gln Val Lys Leu Ile Pro Ile Thr Ser Gln Asn Arg
Ile Pro 340 345 350 Leu Leu Gln Asn Gly Thr Phe Asp Phe Glu Cys Gly
Ser Thr Thr Asn 355 360 365 Asn Val Glu Arg Gln Lys Gln Ala Ala Phe
Ser Asp Thr Ile Phe Val 370 375 380 Val Gly Thr Arg Leu Leu Thr Lys
Lys Gly Gly Asp Ile Lys Asp Phe 385 390 395 400 Ala Asn Leu Lys Asp
Lys Ala Val Val Val Thr Ser Gly Thr Thr Ser 405 410 415 Glu Val Leu
Leu Asn Lys Leu Asn Glu Glu Gln Lys Met Asn Met Arg 420 425 430 Ile
Ile Ser Ala Lys Asp His Gly Asp Ser Phe Arg Thr Leu Glu Ser 435 440
445 Gly Arg Ala Val Ala Phe Met Met Asp Asp Ala Leu Leu Ala Gly Glu
450 455 460 Arg Ala Lys Ala Lys Lys Pro Asp Asn Trp Glu Ile Val Gly
Lys Pro 465 470 475 480 Gln Ser Gln Glu Ala Tyr Gly Cys Met Leu Arg
Lys Asp Asp Pro Gln 485 490 495 Phe Lys Lys Leu Met Asp Asp Thr Ile
Ala Gln Val Gln Thr Ser Gly 500 505 510 Glu Ala Glu Lys Trp Phe Asp
Lys Trp Phe Lys Asn Pro Ile Pro Pro 515 520 525 Lys Asn Leu Asn Met
Asn Phe Glu Leu Ser Asp Glu Met Lys Ala Leu 530 535 540 Phe Lys Glu
Pro Asn Asp Lys Ala Leu Asn Gly Ala Gly Thr Gly Gly 545 550 555 560
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 565
570 575 Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser
Gly 580 585 590 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu
Lys Phe Ile 595 600 605 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro
Thr Leu Val Thr Thr 610 615 620 Phe Gly Tyr Gly Leu Gln Cys Phe Ala
Arg Tyr Pro Asp His Met Lys 625 630 635 640 Gln His Asp Phe Phe Lys
Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 645 650 655 Arg Thr Ile Phe
Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 660 665 670 Val Lys
Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 675 680 685
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 690
695 700 Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys
Asn 705 710 715 720 Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile
Glu Asp Gly Ser 725 730 735 Val Gln Leu Ala Asp His Tyr Gln Gln Asn
Thr Pro Ile Gly Asp Gly 740 745 750 Pro Val Leu Leu Pro Asp Asn His
Tyr Leu Ser Tyr Gln Ser Ala Leu 755 760 765 Ser Lys Asp Pro Asn Glu
Lys Arg Asp His Met Val Leu Leu Glu Phe 770 775 780 Val Thr Ala Ala
Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 785 790 795 <210>
SEQ ID NO 14 <211> LENGTH: 764 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Amino acid sequence of FLIP-E 10u
(expressed as 6xHis fusion in pRSET FLIP-E 10u) <400>
SEQUENCE: 14 Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val
Pro Ile Leu 1 5 10 15 Val Glu Leu Asp Gly Asp Val Asn Gly His Lys
Phe Ser Val Ser Gly 20 25 30 Glu Gly Glu Gly Asp Ala Thr Tyr Gly
Lys Leu Thr Leu Lys Phe Ile 35 40 45 Cys Thr Thr Gly Lys Leu Pro
Val Pro Trp Pro Thr Leu Val Thr Thr 50 55 60 Leu Thr Trp Gly Val
Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 65 70 75 80 Gln His Asp
Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 85 90 95 Arg
Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 100 105
110 Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly
115 120 125 Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu
Glu Tyr 130 135 140 Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp
Lys Gln Lys Asn 145 150 155 160 Gly Ile Lys Ala Asn Phe Lys Ile Arg
His Asn Ile Glu Asp Gly Ser 165 170 175 Val Gln Leu Ala Asp His Tyr
Gln Gln Asn Thr Pro Ile Gly Asp Gly 180 185 190 Pro Val Leu Leu Pro
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 195 200 205 Ser Lys Asp
Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 210 215 220 Val
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 225 230
235 240 Gly Thr Gly Gly Ala Ala Gly Ser Thr Leu Asp Lys Ile Ala Lys
Asn 245 250 255 Gly Val Ile Val Val Gly His Arg Glu Ser Ser Val Pro
Phe Ser Tyr 260 265 270 Tyr Asp Asn Gln Gln Lys Val Val Gly Tyr Ser
Gln Asp Tyr Ser Asn 275 280 285 Ala Ile Val Glu Ala Val Lys Lys Lys
Leu Asn Lys Pro Asp Leu Gln 290 295 300 Val Lys Leu Ile Pro Ile Thr
Ser Gln Asn Arg Ile Pro Leu Leu Gln 305 310 315 320 Asn Gly Thr Phe
Asp Phe Glu Cys Gly Ser Thr Thr Asn Asn Val Glu 325 330 335 Arg Gln
Lys Gln Ala Ala Phe Ser Asp Thr Ile Phe Val Val Gly Thr 340 345 350
Arg Leu Leu Thr Lys Lys Gly Gly Asp Ile Lys Asp Phe Ala Asn Leu 355
360 365 Lys Asp Lys Ala Val Val Val Thr Ser Gly Thr Thr Ser Glu Val
Leu 370 375 380 Leu Asn Lys Leu Asn Glu Glu Gln Lys Met Asn Met Arg
Ile Ile Ser 385 390 395 400 Ala Lys Asp His Gly Asp Ser Phe Arg Thr
Leu Glu Ser Gly Arg Ala 405 410 415 Val Ala Phe Met Met Asp Asp Arg
Leu Leu Ala Gly Glu Arg Ala Lys 420 425 430 Ala Lys Lys Pro Asp Asn
Trp Glu Ile Val Gly Lys Pro Gln Ser Gln 435 440 445 Glu Ala Tyr Gly
Cys Met Leu Arg Lys Asp Asp Pro Gln Phe Lys Lys 450 455 460 Leu Met
Asp Asp Thr Ile Ala Gln Val Gln Thr Ser Gly Glu Ala Glu 465 470 475
480 Lys Trp Phe Asp Lys Trp Phe Lys Asn Pro Ile Pro Pro Lys Asn Leu
485 490 495 Asn Met Asn Phe Glu Leu Ser Asp Glu Met Lys Ala Leu Phe
Lys Glu 500 505 510 Pro Asn Asp Lys Ala Leu Asn Gly Ala Gly Thr Gly
Gly Met Val Ser 515 520 525 Lys Gly Glu Glu Leu Phe Thr Gly Val Val
Pro Ile Leu Val Glu Leu 530 535 540 Asp Gly Asp Val Asn Gly His Lys
Phe Ser Val Ser Gly Glu Gly Glu 545 550 555 560 Gly Asp Ala Thr Tyr
Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 565 570 575 Gly Lys Leu
Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr 580 585 590 Gly
Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp 595 600
605 Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile
610 615 620 Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val
Lys Phe 625 630 635 640 Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu
Lys Gly Ile Asp Phe 645 650 655 Lys Glu Asp Gly Asn Ile Leu Gly His
Lys Leu Glu Tyr Asn Tyr Asn 660 665 670 Ser His Asn Val Tyr Ile Met
Ala Asp Lys Gln Lys Asn Gly Ile Lys 675 680 685 Val Asn Phe Lys Ile
Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 690 695 700 Ala Asp His
Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 705 710 715 720
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp 725
730 735 Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr
Ala 740 745 750 Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 755
760 <210> SEQ ID NO 15 <211> LENGTH: 2496 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION:
FLIP-mglBF16A-Y12S-CFP-D13R-YFP Vector <220> FEATURE:
<221> NAME/KEY: CDS <222> LOCATION: (1)..(2496)
<400> SEQUENCE: 15 atg cgg ggt tct cat cat cat cat cat cat
ggt atg gct agc atg act 48 Met Arg Gly Ser His His His His His His
Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag caa atg ggt cgg gat
ctg tac gac gat gac gat aag gat 96 Gly Gly Gln Gln Met Gly Arg Asp
Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg ggc cgc gct gat act
cgc att ggt gta aca atc tat aag tcg gct 144 Pro Gly Arg Ala Asp Thr
Arg Ile Gly Val Thr Ile Tyr Lys Ser Ala 35 40 45 ggt atg gtg agc
aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc 192 Gly Met Val Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 50 55 60 ctg gtc
gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc 240 Leu Val
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 65 70 75 80
ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ttc 288
Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 85
90 95 atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg
acc 336 Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val
Thr 100 105 110 acc ctg acc tgg ggc gtg cag tgc ttc agc cgc tac ccc
gac cac atg 384 Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro
Asp His Met 115 120 125 aag cag cac gac ttc ttc aag tcc gcc atg ccc
gaa ggc tac gtc cag 432 Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr Val Gln 130 135 140 gag cgc acc atc ttc ttc aag gac gac
ggc aac tac aag acc cgc gcc 480 Glu Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys Thr Arg Ala 145 150 155 160 gag gtg aag ttc gag ggc
gac acc ctg gtg aac cgc atc gag ctg aag 528 Glu Val Lys Phe Glu Gly
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 165 170 175 ggc atc gac ttc
aag gag gac ggc aac atc ctg ggg cac aag ctg gag 576 Gly Ile Asp Phe
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 180 185 190 tac aac
tac atc agc cac aac gtc tat atc acc gcc gac aag cag aag 624 Tyr Asn
Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys 195 200 205
aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc 672
Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 210
215 220 agc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc
gac 720 Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly
Asp 225 230 235 240 ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc
acc cag tcc gcc 768 Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser
Thr Gln Ser Ala 245 250 255 ctg agc aaa gac ccc aac gag aag cgc gat
cac atg gtc ctg ctg gag 816 Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp
His Met Val Leu Leu Glu 260 265 270 ttc gtg acc gcc gcc ggg atc act
ctc ggc atg gac gag ctg tac ggt 864 Phe Val Thr Ala Ala Gly Ile Thr
Leu Gly Met Asp Glu Leu Tyr Gly 275 280 285 agc cga gat aac gcg atg
tct gta gtg cgc aag gct att gag caa gat 912 Ser Arg Asp Asn Ala Met
Ser Val Val Arg Lys Ala Ile Glu Gln Asp 290 295 300 gcg aaa gcc gcg
cca gat gtt cag ctg ctg atg aat gat tct cag aat 960 Ala Lys Ala Ala
Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn 305 310 315 320 gac
cag tcc aag cag aac gat cag atc gac gta ttg ctg gcg aaa ggg 1008
Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly 325
330 335 gtg aag gca ctg gca atc aac ctg gtt gac ccg gca gct gcg ggt
acg 1056 Val Lys Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala
Gly Thr 340 345 350 gtg att gag aaa gcg cgt ggg caa aac gtg ccg gtg
gtt ttc ttc aac 1104 Val Ile Glu Lys Ala Arg Gly Gln Asn Val Pro
Val Val Phe Phe Asn 355 360 365 aaa gaa ccg tct cgt aag gcg ctg gat
agc tac gac aaa gcc tac tac 1152 Lys Glu Pro Ser Arg Lys Ala Leu
Asp Ser Tyr Asp Lys Ala Tyr Tyr 370 375 380 gtt ggc act gac tcc aaa
gag tcc ggc att att caa ggc gat ttg att 1200 Val Gly Thr Asp Ser
Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile 385 390 395 400 gct aaa
cac tgg gcg gcg aat cag ggt tgg gat ctg aac aaa gac ggt 1248 Ala
Lys His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly 405 410
415 cag att cag ttc gta ctg ctg aaa ggt gaa ccg ggc cat ccg gat gca
1296 Gln Ile Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro Asp
Ala 420 425 430 gaa gca cgt acc act tac gtg att aaa gaa ttg aac gat
aaa ggc atc 1344 Glu Ala Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn
Asp Lys Gly Ile 435 440 445 aaa act gaa cag tta cag tta gat acc gca
atg tgg gac acc gct cag 1392 Lys Thr Glu Gln Leu Gln Leu Asp Thr
Ala Met Trp Asp Thr Ala Gln 450 455 460 gcg aaa gat aag atg gac gcc
tgg ctg tct ggc ccg aac gcc aac aaa 1440 Ala Lys Asp Lys Met Asp
Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys 465 470 475 480 atc gaa gtg
gtt atc gcc aac aac gat gcg atg gca atg ggc gcg gtt 1488 Ile Glu
Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val 485 490 495
gaa gcg ctg aaa gca cac aac aag tcc agc att ccg gtg ttt ggc gtc
1536 Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe Gly
Val 500 505 510 gat gcg ctg cca gaa gcg ctg gcg ctg gtg aaa tcc ggt
gca ctg gcg 1584 Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys Ser
Gly Ala Leu Ala 515 520 525 ggc acc gta ctg aac gat gct aac aac cag
gcg aaa gcg acc ttt gat 1632 Gly Thr Val Leu Asn Asp Ala Asn Asn
Gln Ala Lys Ala Thr Phe Asp 530 535 540 ctg gcg aaa aac ctg gcc gat
ggt aaa ggt gcg gct gat ggc acc aac 1680 Leu Ala Lys Asn Leu Ala
Asp Gly Lys Gly Ala Ala Asp Gly Thr Asn 545 550 555 560 tgg aaa atc
gac aac aaa gtg gtc cgc gta cct tat gtt ggc gta gat 1728 Trp Lys
Ile Asp Asn Lys Val Val Arg Val Pro Tyr Val Gly Val Asp 565 570 575
aaa gac aac ctg gct gaa ttc agc aag aaa ggc gcc ggt acc ggt gga
1776 Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys Gly Ala Gly Thr Gly
Gly 580 585 590 atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg
ccc atc ctg 1824 Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val
Val Pro Ile Leu 595 600 605 gtc gag ctg gac ggc gac gta aac ggc cac
aag ttc agc gtg tcc ggc 1872 Val Glu Leu Asp Gly Asp Val Asn Gly
His Lys Phe Ser Val Ser Gly 610 615 620 gag ggc gag ggc gat gcc acc
tac ggc aag ctg acc ctg aag ttc atc 1920 Glu Gly Glu Gly Asp Ala
Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 625 630 635 640 tgc acc acc
ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc 1968 Cys Thr
Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 645 650 655
ttc ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag
2016 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met
Lys 660 665 670 cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac
gtc cag gag 2064 Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly
Tyr Val Gln Glu 675 680 685 cgc acc atc ttc ttc aag gac gac ggc aac
tac aag acc cgc gcc gag 2112 Arg Thr Ile Phe Phe Lys Asp Asp Gly
Asn Tyr Lys Thr Arg Ala Glu 690 695 700 gtg aag ttc gag ggc gac acc
ctg gtg aac cgc atc gag ctg aag ggc 2160 Val Lys Phe Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 705 710 715 720 atc gac ttc
aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac 2208 Ile Asp
Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 725 730 735
aac tac aac agc cac aac gtc tat atc atg gcc gac aag cag aag aac
2256 Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys
Asn 740 745 750 ggc atc aag gtg aac ttc aag atc cgc cac aac atc gag
gac ggc agc 2304 Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile
Glu Asp Gly Ser 755 760 765 gtg cag ctc gcc gac cac tac cag cag aac
acc ccc atc ggc gac ggc 2352 Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile Gly Asp Gly 770 775 780 ccc gtg ctg ctg ccc gac aac
cac tac ctg agc tac cag tcc gcc ctg 2400 Pro Val Leu Leu Pro Asp
Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 785 790 795 800 agc aaa gac
ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc 2448 Ser Lys
Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 805 810 815
gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag taa
2496 Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys
820 825 830 <210> SEQ ID NO 16 <211> LENGTH: 831
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION:
FLIP-mglBF16A-Y12S-CFP-D13R-YFP Vector <400> SEQUENCE: 16 Met
Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5 10
15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp
20 25 30 Pro Gly Arg Ala Asp Thr Arg Ile Gly Val Thr Ile Tyr Lys
Ser Ala 35 40 45 Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val Val Pro Ile 50 55 60 Leu Val Glu Leu Asp Gly Asp Val Asn Gly
His Lys Phe Ser Val Ser 65 70 75 80 Gly Glu Gly Glu Gly Asp Ala Thr
Tyr Gly Lys Leu Thr Leu Lys Phe 85 90 95 Ile Cys Thr Thr Gly Lys
Leu Pro Val Pro Trp Pro Thr Leu Val Thr 100 105 110 Thr Leu Thr Trp
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 115 120 125 Lys Gln
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 130 135 140
Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 145
150 155 160 Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu
Leu Lys 165 170 175 Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
His Lys Leu Glu 180 185 190 Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile
Thr Ala Asp Lys Gln Lys 195 200 205 Asn Gly Ile Lys Ala Asn Phe Lys
Ile Arg His Asn Ile Glu Asp Gly 210 215 220 Ser Val Gln Leu Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 225 230 235 240 Gly Pro Val
Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 245 250 255 Leu
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 260 265
270 Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Gly
275 280 285 Ser Arg Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile Glu
Gln Asp 290 295 300 Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn
Asp Ser Gln Asn 305 310 315 320 Asp Gln Ser Lys Gln Asn Asp Gln Ile
Asp Val Leu Leu Ala Lys Gly 325 330 335 Val Lys Ala Leu Ala Ile Asn
Leu Val Asp Pro Ala Ala Ala Gly Thr 340 345 350 Val Ile Glu Lys Ala
Arg Gly Gln Asn Val Pro Val Val Phe Phe Asn 355 360 365 Lys Glu Pro
Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr 370 375 380 Val
Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile 385 390
395 400 Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp
Gly 405 410 415 Gln Ile Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His
Pro Asp Ala 420 425 430 Glu Ala Arg Thr Thr Tyr Val Ile Lys Glu Leu
Asn Asp Lys Gly Ile 435 440 445 Lys Thr Glu Gln Leu Gln Leu Asp Thr
Ala Met Trp Asp Thr Ala Gln 450 455 460 Ala Lys Asp Lys Met Asp Ala
Trp Leu Ser Gly Pro Asn Ala Asn Lys 465 470 475 480 Ile Glu Val Val
Ile Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val 485 490 495 Glu Ala
Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe Gly Val 500 505 510
Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala 515
520 525 Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe
Asp 530 535 540 Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala Ala Asp
Gly Thr Asn 545 550 555 560 Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro Tyr Val Gly Val Asp 565 570 575 Lys Asp Asn Leu Ala Glu Phe Ser
Lys Lys Gly Ala Gly Thr Gly Gly 580 585 590 Met Val Ser Lys Gly Glu
Glu Leu Phe Thr Gly Val Val Pro Ile Leu 595 600 605 Val Glu Leu Asp
Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 610 615 620 Glu Gly
Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 625 630 635
640 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr
645 650 655 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His
Met Lys 660 665 670 Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly
Tyr Val Gln Glu 675 680 685 Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn
Tyr Lys Thr Arg Ala Glu 690 695 700 Val Lys Phe Glu Gly Asp Thr Leu
Val Asn Arg Ile Glu Leu Lys Gly 705 710 715 720 Ile Asp Phe Lys Glu
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 725 730 735 Asn Tyr Asn
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 740 745 750 Gly
Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 755 760
765 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly
770 775 780 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser
Ala Leu 785 790 795 800 Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met
Val Leu Leu Glu Phe 805 810 815 Val Thr Ala Ala Gly Ile Thr Leu Gly
Met Asp Glu Leu Tyr Lys 820 825 830 <210> SEQ ID NO 17
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-mglBF16A-G275S-CFP-K276R-YFP Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 17 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc gct gat act cgc att ggt gta aca atc tat aag tac gac 144 Pro
Gly Arg Ala Asp Thr Arg Ile Gly Val Thr Ile Tyr Lys Tyr Asp 35 40
45 gat aac gcg atg tct gta gtg cgc aag gct att gag caa gat gcg aaa
192 Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys
50 55 60 gcc gcg cca gat gtt cag ctg ctg atg aat gat tct cag aat
gac cag 240 Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn
Asp Gln 65 70 75 80 tcc aag cag aac gat cag atc gac gta ttg ctg gcg
aaa ggg gtg aag 288 Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala
Lys Gly Val Lys 85 90 95 gca ctg gca atc aac ctg gtt gac ccg gca
gct gcg ggt acg gtg att 336 Ala Leu Ala Ile Asn Leu Val Asp Pro Ala
Ala Ala Gly Thr Val Ile 100 105 110 gag aaa gcg cgt ggg caa aac gtg
ccg gtg gtt ttc ttc aac aaa gaa 384 Glu Lys Ala Arg Gly Gln Asn Val
Pro Val Val Phe Phe Asn Lys Glu 115 120 125 ccg tct cgt aag gcg ctg
gat agc tac gac aaa gcc tac tac gtt ggc 432 Pro Ser Arg Lys Ala Leu
Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly 130 135 140 act gac tcc aaa
gag tcc ggc att att caa ggc gat ttg att gct aaa 480 Thr Asp Ser Lys
Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys 145 150 155 160 cac
tgg gcg gcg aat cag ggt tgg gat ctg aac aaa gac ggt cag att 528 His
Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly Gln Ile 165 170
175 cag ttc gta ctg ctg aaa ggt gaa ccg ggc cat ccg gat gca gaa gca
576 Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro Asp Ala Glu Ala
180 185 190 cgt acc act tac gtg att aaa gaa ttg aac gat aaa ggc atc
aaa act 624 Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile
Lys Thr 195 200 205 gaa cag tta cag tta gat acc gca atg tgg gac acc
gct cag gcg aaa 672 Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr
Ala Gln Ala Lys 210 215 220 gat aag atg gac gcc tgg ctg tct ggc ccg
aac gcc aac aaa atc gaa 720 Asp Lys Met Asp Ala Trp Leu Ser Gly Pro
Asn Ala Asn Lys Ile Glu 225 230 235 240 gtg gtt atc gcc aac aac gat
gcg atg gca atg ggc gcg gtt gaa gcg 768 Val Val Ile Ala Asn Asn Asp
Ala Met Ala Met Gly Ala Val Glu Ala 245 250 255 ctg aaa gca cac aac
aag tcc agc att ccg gtg ttt ggc gtc gat gcg 816 Leu Lys Ala His Asn
Lys Ser Ser Ile Pro Val Phe Gly Val Asp Ala 260 265 270 ctg cca gaa
gcg ctg gcg ctg gtg aaa tcc ggt gca ctg gcg ggc acc 864 Leu Pro Glu
Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala Gly Thr 275 280 285 gta
ctg aac gat gct aac aac cag gcg aaa gcg acc ttt gat ctg gcg 912 Val
Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe Asp Leu Ala 290 295
300 aaa aac ctg gcc gat tcg gct ggt atg gtg agc aag ggc gag gag ctg
960 Lys Asn Leu Ala Asp Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu
305 310 315 320 ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc
gac gta aac 1008 Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp
Gly Asp Val Asn 325 330 335 ggc cac aag ttc agc gtg tcc ggc gag ggc
gag ggc gat gcc acc tac 1056 Gly His Lys Phe Ser Val Ser Gly Glu
Gly Glu Gly Asp Ala Thr Tyr 340 345 350 ggc aag ctg acc ctg aag ttc
atc tgc acc acc ggc aag ctg ccc gtg 1104 Gly Lys Leu Thr Leu Lys
Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 355 360 365 ccc tgg ccc acc
ctc gtg acc acc ctg acc tgg ggc gtg cag tgc ttc 1152 Pro Trp Pro
Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe 370 375 380 agc
cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc 1200
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 385
390 395 400 atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag
gac gac 1248 Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
Lys Asp Asp 405 410 415 ggc aac tac aag acc cgc gcc gag gtg aag ttc
gag ggc gac acc ctg 1296 Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys
Phe Glu Gly Asp Thr Leu 420 425 430 gtg aac cgc atc gag ctg aag ggc
atc gac ttc aag gag gac ggc aac 1344 Val Asn Arg Ile Glu Leu Lys
Gly Ile Asp Phe Lys Glu Asp Gly Asn 435 440 445 atc ctg ggg cac aag
ctg gag tac aac tac atc agc cac aac gtc tat 1392 Ile Leu Gly His
Lys Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr 450 455 460 atc acc
gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc 1440 Ile
Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 465 470
475 480 cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac cac tac
cag 1488 Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His
Tyr Gln 485 490 495 cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg
ccc gac aac cac 1536 Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu
Leu Pro Asp Asn His 500 505 510 tac ctg agc acc cag tcc gcc ctg agc
aaa gac ccc aac gag aag cgc 1584 Tyr Leu Ser Thr Gln Ser Ala Leu
Ser Lys Asp Pro Asn Glu Lys Arg 515 520 525 gat cac atg gtc ctg ctg
gag ttc gtg acc gcc gcc ggg atc act ctc 1632 Asp His Met Val Leu
Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 530 535 540 ggc atg gac
gag ctg tac ggt agc cga ggt gcg gct gat ggc acc aac 1680 Gly Met
Asp Glu Leu Tyr Gly Ser Arg Gly Ala Ala Asp Gly Thr Asn 545 550 555
560 tgg aaa atc gac aac aaa gtg gtc cgc gta cct tat gtt ggc gta gat
1728 Trp Lys Ile Asp Asn Lys Val Val Arg Val Pro Tyr Val Gly Val
Asp 565 570 575 aaa gac aac ctg gct gaa ttc agc aag aaa ggc gcc ggt
acc ggt gga 1776 Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys Gly Ala
Gly Thr Gly Gly 580 585 590 atg gtg agc aag ggc gag gag ctg ttc acc
ggg gtg gtg ccc atc ctg 1824 Met Val Ser Lys Gly Glu Glu Leu Phe
Thr Gly Val Val Pro Ile Leu 595 600 605 gtc gag ctg gac ggc gac gta
aac ggc cac aag ttc agc gtg tcc ggc 1872 Val Glu Leu Asp Gly Asp
Val Asn Gly His Lys Phe Ser Val Ser Gly 610 615 620 gag ggc gag ggc
gat gcc acc tac ggc aag ctg acc ctg aag ttc atc 1920 Glu Gly Glu
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 625 630 635 640
tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc
1968 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr
Thr 645 650 655 ttc ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac
cac atg aag 2016 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro
Asp His Met Lys 660 665 670 cag cac gac ttc ttc aag tcc gcc atg ccc
gaa ggc tac gtc cag gag 2064 Gln His Asp Phe Phe Lys Ser Ala Met
Pro Glu Gly Tyr Val Gln Glu 675 680 685 cgc acc atc ttc ttc aag gac
gac ggc aac tac aag acc cgc gcc gag 2112 Arg Thr Ile Phe Phe Lys
Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 690 695 700 gtg aag ttc gag
ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc 2160 Val Lys Phe
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 705 710 715 720
atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac
2208 Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu
Tyr 725 730 735 aac tac aac agc cac aac gtc tat atc atg gcc gac aag
cag aag aac 2256 Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp
Lys Gln Lys Asn 740 745 750 ggc atc aag gtg aac ttc aag atc cgc cac
aac atc gag gac ggc agc 2304 Gly Ile Lys Val Asn Phe Lys Ile Arg
His Asn Ile Glu Asp Gly Ser 755 760 765 gtg cag ctc gcc gac cac tac
cag cag aac acc ccc atc ggc gac ggc 2352 Val Gln Leu Ala Asp His
Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 770 775 780 ccc gtg ctg ctg
ccc gac aac cac tac ctg agc tac cag tcc gcc ctg 2400 Pro Val Leu
Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 785 790 795 800
agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc
2448 Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu
Phe 805 810 815 gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg
tac aag taa 2496 Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu
Leu Tyr Lys 820 825 830 <210> SEQ ID NO 18 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-mglBF16A-G275S-CFP-K276R-YFP Vector <400> SEQUENCE: 18
Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5
10 15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys
Asp 20 25 30 Pro Gly Arg Ala Asp Thr Arg Ile Gly Val Thr Ile Tyr
Lys Tyr Asp 35 40 45 Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile
Glu Gln Asp Ala Lys 50 55 60 Ala Ala Pro Asp Val Gln Leu Leu Met
Asn Asp Ser Gln Asn Asp Gln 65 70 75 80 Ser Lys Gln Asn Asp Gln Ile
Asp Val Leu Leu Ala Lys Gly Val Lys 85 90 95 Ala Leu Ala Ile Asn
Leu Val Asp Pro Ala Ala Ala Gly Thr Val Ile 100 105 110 Glu Lys Ala
Arg Gly Gln Asn Val Pro Val Val Phe Phe Asn Lys Glu 115 120 125 Pro
Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly 130 135
140 Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys
145 150 155 160 His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp
Gly Gln Ile 165 170 175 Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His
Pro Asp Ala Glu Ala 180 185 190 Arg Thr Thr Tyr Val Ile Lys Glu Leu
Asn Asp Lys Gly Ile Lys Thr 195 200 205 Glu Gln Leu Gln Leu Asp Thr
Ala Met Trp Asp Thr Ala Gln Ala Lys 210 215 220 Asp Lys Met Asp Ala
Trp Leu Ser Gly Pro Asn Ala Asn Lys Ile Glu 225 230 235 240 Val Val
Ile Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val Glu Ala 245 250 255
Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe Gly Val Asp Ala 260
265 270 Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala Gly
Thr 275 280 285 Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe
Asp Leu Ala 290 295 300 Lys Asn Leu Ala Asp Ser Ala Gly Met Val Ser
Lys Gly Glu Glu Leu 305 310 315 320 Phe Thr Gly Val Val Pro Ile Leu
Val Glu Leu Asp Gly Asp Val Asn 325 330 335 Gly His Lys Phe Ser Val
Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 340 345 350 Gly Lys Leu Thr
Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 355 360 365 Pro Trp
Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe 370 375 380
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 385
390 395 400 Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys
Asp Asp 405 410 415 Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu
Gly Asp Thr Leu 420 425 430 Val Asn Arg Ile Glu Leu Lys Gly Ile Asp
Phe Lys Glu Asp Gly Asn 435 440 445 Ile Leu Gly His Lys Leu Glu Tyr
Asn Tyr Ile Ser His Asn Val Tyr 450 455 460 Ile Thr Ala Asp Lys Gln
Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 465 470 475 480 Arg His Asn
Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 485 490 495 Gln
Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 500 505
510 Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg
515 520 525 Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile
Thr Leu 530 535 540 Gly Met Asp Glu Leu Tyr Gly Ser Arg Gly Ala Ala
Asp Gly Thr Asn 545 550 555 560 Trp Lys Ile Asp Asn Lys Val Val Arg
Val Pro Tyr Val Gly Val Asp 565 570 575 Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys Gly Ala Gly Thr Gly Gly 580 585 590 Met Val Ser Lys Gly
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 595 600 605 Val Glu Leu
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 610 615 620 Glu
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 625 630
635 640 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr
Thr 645 650 655 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp
His Met Lys 660 665 670 Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu
Gly Tyr Val Gln Glu 675 680 685 Arg Thr Ile Phe Phe Lys Asp Asp Gly
Asn Tyr Lys Thr Arg Ala Glu 690 695 700 Val Lys Phe Glu Gly Asp Thr
Leu Val Asn Arg Ile Glu Leu Lys Gly 705 710 715 720 Ile Asp Phe Lys
Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 725 730 735 Asn Tyr
Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 740 745 750
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 755
760 765 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly 770 775 780 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln
Ser Ala Leu 785 790 795 800 Ser Lys Asp Pro Asn Glu Lys Arg Asp His
Met Val Leu Leu Glu Phe 805 810 815 Val Thr Ala Ala Gly Ile Thr Leu
Gly Met Asp Glu Leu Tyr Lys 820 825 830 <210> SEQ ID NO 19
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-Y12S-CFP-D13R Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 19 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tcg gct ggt atg gtg agc aag ggc gag gag ctg ttc acc 912 Ile
Tyr Lys Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr 290 295
300 ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac
960 Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His
305 310 315 320 aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc
tac ggc aag 1008 Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala
Thr Tyr Gly Lys 325 330 335 ctg acc ctg aag ttc atc tgc acc acc ggc
aag ctg ccc gtg ccc tgg 1056 Leu Thr Leu Lys Phe Ile Cys Thr Thr
Gly Lys Leu Pro Val Pro Trp 340 345 350 ccc acc ctc gtg acc acc ctg
acc tgg ggc gtg cag tgc ttc agc cgc 1104 Pro Thr Leu Val Thr Thr
Leu Thr Trp Gly Val Gln Cys Phe Ser Arg 355 360 365 tac ccc gac cac
atg aag cag cac gac ttc ttc aag tcc gcc atg ccc 1152 Tyr Pro Asp
His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 370 375 380 gaa
ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac 1200
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn 385
390 395 400 tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg
gtg aac 1248 Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr
Leu Val Asn 405 410 415 cgc atc gag ctg aag ggc atc gac ttc aag gag
gac ggc aac atc ctg 1296 Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys
Glu Asp Gly Asn Ile Leu 420 425 430 ggg cac aag ctg gag tac aac tac
atc agc cac aac gtc tat atc acc 1344 Gly His Lys Leu Glu Tyr Asn
Tyr Ile Ser His Asn Val Tyr Ile Thr 435 440 445 gcc gac aag cag aag
aac ggc atc aag gcc aac ttc aag atc cgc cac 1392 Ala Asp Lys Gln
Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His 450 455 460 aac atc
gag gac ggc agc gtg cag ctc gcc gac cac tac cag cag aac 1440 Asn
Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn 465 470
475 480 acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac
ctg 1488 Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His
Tyr Leu 485 490 495 agc acc cag tcc gcc ctg agc aaa gac ccc aac gag
aag cgc gat cac 1536 Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
Glu Lys Arg Asp His 500 505 510 atg gtc ctg ctg gag ttc gtg acc gcc
gcc ggg atc act ctc ggc atg 1584 Met Val Leu Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met 515 520 525 gac gag ctg tac ggt agc
cga gat aac gcg atg tct gta gtg cgc aag 1632 Asp Glu Leu Tyr Gly
Ser Arg Asp Asn Ala Met Ser Val Val Arg Lys 530 535 540 gct att gag
caa gat gcg aaa gcc gcg cca gat gtt cag ctg ctg atg 1680 Ala Ile
Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met 545 550 555
560 aat gat tct cag aat gac cag tcc aag cag aac gat cag atc gac gta
1728 Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp
Val 565 570 575 ttg ctg gcg aaa ggg gtg aag gca ctg gca atc aac ctg
gtt gac ccg 1776 Leu Leu Ala Lys Gly Val Lys Ala Leu Ala Ile Asn
Leu Val Asp Pro 580 585 590 gca gct gcg ggt acg gtg att gag aaa gcg
cgt ggg caa aac gtg ccg 1824 Ala Ala Ala Gly Thr Val Ile Glu Lys
Ala Arg Gly Gln Asn Val Pro 595 600 605 gtg gtt ttc ttc aac aaa gaa
ccg tct cgt aag gcg ctg gat agc tac 1872 Val Val Phe Phe Asn Lys
Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr 610 615 620 gac aaa gcc tac
tac gtt ggc act gac tcc aaa gag tcc ggc att att 1920 Asp Lys Ala
Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile 625 630 635 640
caa ggc gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
1968 Gln Gly Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp 645 650 655 ctg aac aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg 2016 Leu Asn Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro 660 665 670 ggc cat ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg 2064 Gly His Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu 675 680 685 aac gat aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg 2112 Asn Asp Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met 690 695 700 tgg gac acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc 2160 Trp Asp Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly 705 710 715 720
ccg aac gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg
2208 Pro Asn Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala
Met 725 730 735 gca atg ggc gcg gtt gaa gcg ctg aaa gca cac aac aag
tcc agc att 2256 Ala Met Gly Ala Val Glu Ala Leu Lys Ala His Asn
Lys Ser Ser Ile 740 745 750 ccg gtg ttt ggc gtc gat gcg ctg cca gaa
gcg ctg gcg ctg gtg aaa 2304 Pro Val Phe Gly Val Asp Ala Leu Pro
Glu Ala Leu Ala Leu Val Lys 755 760 765 tcc ggt gca ctg gcg ggc acc
gta ctg aac gat gct aac aac cag gcg 2352 Ser Gly Ala Leu Ala Gly
Thr Val Leu Asn Asp Ala Asn Asn Gln Ala 770 775 780 aaa gcg acc ttt
gat ctg gcg aaa aac ctg gcc gat ggt aaa ggt gcg 2400 Lys Ala Thr
Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala 785 790 795 800
gct gat ggc acc aac tgg aaa atc gac aac aaa gtg gtc cgc gta cct
2448 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro 805 810 815 tat gtt ggc gta gat aaa gac aac ctg gct gaa ttc agc
aag aaa taa 2496 Tyr Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys 820 825 830 <210> SEQ ID NO 20 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-YFP-mglBF16A-Y12S-CFP-D13R Vector <400> SEQUENCE: 20 Met
Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5 10
15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp
20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly
His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr
Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile Cys Thr Thr Gly Lys
Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val Thr Thr Leu Thr Trp
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105 110 His Met Lys Gln
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 Val Gln
Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145
150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile
Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys
Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser Val Gln Leu Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly Asp Gly Pro Val Leu
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230 235 240 Ser Ala Leu
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 Leu
Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265
270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr
275 280 285 Ile Tyr Lys Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu
Phe Thr 290 295 300 Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp
Val Asn Gly His 305 310 315 320 Lys Phe Ser Val Ser Gly Glu Gly Glu
Gly Asp Ala Thr Tyr Gly Lys 325 330 335 Leu Thr Leu Lys Phe Ile Cys
Thr Thr Gly Lys Leu Pro Val Pro Trp 340 345 350 Pro Thr Leu Val Thr
Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg 355 360 365 Tyr Pro Asp
His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 370 375 380 Glu
Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn 385 390
395 400 Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val
Asn 405 410 415 Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly
Asn Ile Leu 420 425 430 Gly His Lys Leu Glu Tyr Asn Tyr Ile Ser His
Asn Val Tyr Ile Thr 435 440 445 Ala Asp Lys Gln Lys Asn Gly Ile Lys
Ala Asn Phe Lys Ile Arg His 450 455 460 Asn Ile Glu Asp Gly Ser Val
Gln Leu Ala Asp His Tyr Gln Gln Asn 465 470 475 480 Thr Pro Ile Gly
Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu 485 490 495 Ser Thr
Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His 500 505 510
Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met 515
520 525 Asp Glu Leu Tyr Gly Ser Arg Asp Asn Ala Met Ser Val Val Arg
Lys 530 535 540 Ala Ile Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln
Leu Leu Met 545 550 555 560 Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln
Asn Asp Gln Ile Asp Val 565 570 575 Leu Leu Ala Lys Gly Val Lys Ala
Leu Ala Ile Asn Leu Val Asp Pro 580 585 590 Ala Ala Ala Gly Thr Val
Ile Glu Lys Ala Arg Gly Gln Asn Val Pro 595 600 605 Val Val Phe Phe
Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr 610 615 620 Asp Lys
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile 625 630 635
640 Gln Gly Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp
645 650 655 Leu Asn Lys Asp Gly Gln Ile Gln Phe Val Leu Leu Lys Gly
Glu Pro 660 665 670 Gly His Pro Asp Ala Glu Ala Arg Thr Thr Tyr Val
Ile Lys Glu Leu 675 680 685 Asn Asp Lys Gly Ile Lys Thr Glu Gln Leu
Gln Leu Asp Thr Ala Met 690 695 700 Trp Asp Thr Ala Gln Ala Lys Asp
Lys Met Asp Ala Trp Leu Ser Gly 705 710 715 720 Pro Asn Ala Asn Lys
Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met 725 730 735 Ala Met Gly
Ala Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile 740 745 750 Pro
Val Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys 755 760
765 Ser Gly Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala
770 775 780 Lys Ala Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys
Gly Ala 785 790 795 800 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn Lys
Val Val Arg Val Pro 805 810 815 Tyr Val Gly Val Asp Lys Asp Asn Leu
Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ ID NO 21
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-P32S-CFP-D33R Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 21 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tac gac gat aac gcg atg tct gta gtg cgc aag gct att 912 Ile
Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295
300 gag caa gat gcg aaa gcc gcg tcg gct ggt atg gtg agc aag ggc gag
960 Glu Gln Asp Ala Lys Ala Ala Ser Ala Gly Met Val Ser Lys Gly Glu
305 310 315 320 gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg
gac ggc gac 1008 Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu
Leu Asp Gly Asp 325 330 335 gta aac ggc cac aag ttc agc gtg tcc ggc
gag ggc gag ggc gat gcc 1056 Val Asn Gly His Lys Phe Ser Val Ser
Gly Glu Gly Glu Gly Asp Ala 340 345 350 acc tac ggc aag ctg acc ctg
aag ttc atc tgc acc acc ggc aag ctg 1104 Thr Tyr Gly Lys Leu Thr
Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 355 360 365 ccc gtg ccc tgg
ccc acc ctc gtg acc acc ctg acc tgg ggc gtg cag 1152 Pro Val Pro
Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln 370 375 380 tgc
ttc agc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag 1200
Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 385
390 395 400 tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc
ttc aag 1248 Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile
Phe Phe Lys 405 410 415 gac gac ggc aac tac aag acc cgc gcc gag gtg
aag ttc gag ggc gac 1296 Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu
Val Lys Phe Glu Gly Asp 420 425 430 acc ctg gtg aac cgc atc gag ctg
aag ggc atc gac ttc aag gag gac 1344 Thr Leu Val Asn Arg Ile Glu
Leu Lys Gly Ile Asp Phe Lys Glu Asp 435 440 445 ggc aac atc ctg ggg
cac aag ctg gag tac aac tac atc agc cac aac 1392 Gly Asn Ile Leu
Gly His Lys Leu Glu Tyr Asn Tyr Ile Ser His Asn 450 455 460 gtc tat
atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc 1440 Val
Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe 465 470
475 480 aag atc cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac
cac 1488 Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala
Asp His 485 490 495 tac cag cag aac acc ccc atc ggc gac ggc ccc gtg
ctg ctg ccc gac 1536 Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro
Val Leu Leu Pro Asp 500 505 510 aac cac tac ctg agc acc cag tcc gcc
ctg agc aaa gac ccc aac gag 1584 Asn His Tyr Leu Ser Thr Gln Ser
Ala Leu Ser Lys Asp Pro Asn Glu 515 520 525 aag cgc gat cac atg gtc
ctg ctg gag ttc gtg acc gcc gcc ggg atc 1632 Lys Arg Asp His Met
Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 530 535 540 act ctc ggc
atg gac gag ctg tac ggt agc cga gtt cag ctg ctg atg 1680 Thr Leu
Gly Met Asp Glu Leu Tyr Gly Ser Arg Val Gln Leu Leu Met 545 550 555
560 aat gat tct cag aat gac cag tcc aag cag aac gat cag atc gac gta
1728 Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp
Val 565 570 575 ttg ctg gcg aaa ggg gtg aag gca ctg gca atc aac ctg
gtt gac ccg 1776 Leu Leu Ala Lys Gly Val Lys Ala Leu Ala Ile Asn
Leu Val Asp Pro 580 585 590 gca gct gcg ggt acg gtg att gag aaa gcg
cgt ggg caa aac gtg ccg 1824 Ala Ala Ala Gly Thr Val Ile Glu Lys
Ala Arg Gly Gln Asn Val Pro 595 600 605 gtg gtt ttc ttc aac aaa gaa
ccg tct cgt aag gcg ctg gat agc tac 1872 Val Val Phe Phe Asn Lys
Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr 610 615 620 gac aaa gcc tac
tac gtt ggc act gac tcc aaa gag tcc ggc att att 1920 Asp Lys Ala
Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile 625 630 635 640
caa ggc gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
1968 Gln Gly Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp 645 650 655 ctg aac aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg 2016 Leu Asn Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro 660 665 670 ggc cat ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg 2064 Gly His Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu 675 680 685 aac gat aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg 2112 Asn Asp Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met 690 695 700 tgg gac acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc 2160 Trp Asp Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly 705 710 715 720
ccg aac gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg
2208 Pro Asn Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala
Met 725 730 735 gca atg ggc gcg gtt gaa gcg ctg aaa gca cac aac aag
tcc agc att 2256 Ala Met Gly Ala Val Glu Ala Leu Lys Ala His Asn
Lys Ser Ser Ile 740 745 750 ccg gtg ttt ggc gtc gat gcg ctg cca gaa
gcg ctg gcg ctg gtg aaa 2304 Pro Val Phe Gly Val Asp Ala Leu Pro
Glu Ala Leu Ala Leu Val Lys 755 760 765 tcc ggt gca ctg gcg ggc acc
gta ctg aac gat gct aac aac cag gcg 2352 Ser Gly Ala Leu Ala Gly
Thr Val Leu Asn Asp Ala Asn Asn Gln Ala 770 775 780 aaa gcg acc ttt
gat ctg gcg aaa aac ctg gcc gat ggt aaa ggt gcg 2400 Lys Ala Thr
Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala 785 790 795 800
gct gat ggc acc aac tgg aaa atc gac aac aaa gtg gtc cgc gta cct
2448 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro 805 810 815 tat gtt ggc gta gat aaa gac aac ctg gct gaa ttc agc
aag aaa taa 2496 Tyr Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys 820 825 830 <210> SEQ ID NO 22 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-YFP-mglBF16A-P32S-CFP-D33R Vector <400> SEQUENCE: 22 Met
Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5 10
15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp
20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly
His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr
Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile Cys Thr Thr Gly Lys
Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val Thr Thr Leu Thr Trp
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105 110 His Met Lys Gln
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 Val Gln
Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145
150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile
Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys
Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser Val Gln Leu Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly Asp Gly Pro Val Leu
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230 235 240 Ser Ala Leu
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 Leu
Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265
270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr
275 280 285 Ile Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys
Ala Ile 290 295 300 Glu Gln Asp Ala Lys Ala Ala Ser Ala Gly Met Val
Ser Lys Gly Glu 305 310 315 320 Glu Leu Phe Thr Gly Val Val Pro Ile
Leu Val Glu Leu Asp Gly Asp 325 330 335 Val Asn Gly His Lys Phe Ser
Val Ser Gly Glu Gly Glu Gly Asp Ala 340 345 350 Thr Tyr Gly Lys Leu
Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 355 360 365 Pro Val Pro
Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln 370 375 380 Cys
Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 385 390
395 400 Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
Lys 405 410 415 Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe
Glu Gly Asp 420 425 430 Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile
Asp Phe Lys Glu Asp 435 440 445 Gly Asn Ile Leu Gly His Lys Leu Glu
Tyr Asn Tyr Ile Ser His Asn 450 455 460 Val Tyr Ile Thr Ala Asp Lys
Gln Lys Asn Gly Ile Lys Ala Asn Phe 465 470 475 480 Lys Ile Arg His
Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 485 490 495 Tyr Gln
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 500 505 510
Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu 515
520 525 Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
Ile 530 535 540 Thr Leu Gly Met Asp Glu Leu Tyr Gly Ser Arg Val Gln
Leu Leu Met 545 550 555 560 Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln
Asn Asp Gln Ile Asp Val 565 570 575 Leu Leu Ala Lys Gly Val Lys Ala
Leu Ala Ile Asn Leu Val Asp Pro 580 585 590 Ala Ala Ala Gly Thr Val
Ile Glu Lys Ala Arg Gly Gln Asn Val Pro 595 600 605 Val Val Phe Phe
Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr 610 615 620 Asp Lys
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile 625 630 635
640 Gln Gly Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp
645 650 655 Leu Asn Lys Asp Gly Gln Ile Gln Phe Val Leu Leu Lys Gly
Glu Pro 660 665 670 Gly His Pro Asp Ala Glu Ala Arg Thr Thr Tyr Val
Ile Lys Glu Leu 675 680 685 Asn Asp Lys Gly Ile Lys Thr Glu Gln Leu
Gln Leu Asp Thr Ala Met 690 695 700 Trp Asp Thr Ala Gln Ala Lys Asp
Lys Met Asp Ala Trp Leu Ser Gly 705 710 715 720 Pro Asn Ala Asn Lys
Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met 725 730 735 Ala Met Gly
Ala Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile 740 745 750 Pro
Val Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys 755 760
765 Ser Gly Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala
770 775 780 Lys Ala Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys
Gly Ala 785 790 795 800 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn Lys
Val Val Arg Val Pro 805 810 815 Tyr Val Gly Val Asp Lys Asp Asn Leu
Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ ID NO 23
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-G275S-CFP-K276R Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 23 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tac gac gat aac gcg atg tct gta gtg cgc aag gct att 912 Ile
Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295
300 gag caa gat gcg aaa gcc gcg cca gat gtt cag ctg ctg atg aat gat
960 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp
305 310 315 320 tct cag aat gac cag tcc aag cag aac gat cag atc gac
gta ttg ctg 1008 Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile
Asp Val Leu Leu 325 330 335 gcg aaa ggg gtg aag gca ctg gca atc aac
ctg gtt gac ccg gca gct 1056 Ala Lys Gly Val Lys Ala Leu Ala Ile
Asn Leu Val Asp Pro Ala Ala 340 345 350 gcg ggt acg gtg att gag aaa
gcg cgt ggg caa aac gtg ccg gtg gtt 1104 Ala Gly Thr Val Ile Glu
Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 ttc ttc aac aaa
gaa ccg tct cgt aag gcg ctg gat agc tac gac aaa 1152 Phe Phe Asn
Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380 gcc
tac tac gtt ggc act gac tcc aaa gag tcc ggc att att caa ggc 1200
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385
390 395 400 gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
ctg aac 1248 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp Leu Asn 405 410 415 aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg ggc cat 1296 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro Gly His 420 425 430 ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg aac gat 1344 Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu Asn Asp 435 440 445 aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg tgg gac 1392 Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp 450 455 460 acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc ccg aac 1440 Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470
475 480 gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg gca
atg 1488 Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met
Ala Met 485 490 495 ggc gcg gtt gaa gcg ctg aaa gca cac aac aag tcc
agc att ccg gtg 1536 Gly Ala Val Glu Ala Leu Lys Ala His Asn Lys
Ser Ser Ile Pro Val 500 505 510 ttt ggc gtc gat gcg ctg cca gaa gcg
ctg gcg ctg gtg aaa tcc ggt 1584 Phe Gly Val Asp Ala Leu Pro Glu
Ala Leu Ala Leu Val Lys Ser Gly 515 520 525 gca ctg gcg ggc acc gta
ctg aac gat gct aac aac cag gcg aaa gcg 1632 Ala Leu Ala Gly Thr
Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala 530 535 540 acc ttt gat
ctg gcg aaa aac ctg gcc gat tcg gct ggt atg gtg agc 1680 Thr Phe
Asp Leu Ala Lys Asn Leu Ala Asp Ser Ala Gly Met Val Ser 545 550 555
560 aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg
1728 Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu
Leu 565 570 575 gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc
gag ggc gag 1776 Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser
Gly Glu Gly Glu 580 585 590 ggc gat gcc acc tac ggc aag ctg acc ctg
aag ttc atc tgc acc acc 1824 Gly Asp Ala Thr Tyr Gly Lys Leu Thr
Leu Lys Phe Ile Cys Thr Thr 595 600 605 ggc aag ctg ccc gtg ccc tgg
ccc acc ctc gtg acc acc ctg acc tgg 1872 Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu Val Thr Thr Leu Thr Trp 610 615 620 ggc gtg cag tgc
ttc agc cgc tac ccc gac cac atg aag cag cac gac 1920 Gly Val Gln
Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 625 630 635 640
ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc
1968 Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr
Ile 645 650 655 ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag
gtg aag ttc 2016 Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala
Glu Val Lys Phe 660 665 670 gag ggc gac acc ctg gtg aac cgc atc gag
ctg aag ggc atc gac ttc 2064 Glu Gly Asp Thr Leu Val Asn Arg Ile
Glu Leu Lys Gly Ile Asp Phe 675 680 685 aag gag gac ggc aac atc ctg
ggg cac aag ctg gag tac aac tac atc 2112 Lys Glu Asp Gly Asn Ile
Leu Gly His Lys Leu Glu Tyr Asn Tyr Ile 690 695 700 agc cac aac gtc
tat atc acc gcc gac aag cag aag aac ggc atc aag 2160 Ser His Asn
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys 705 710 715 720
gcc aac ttc aag atc cgc cac aac atc gag gac ggc agc gtg cag ctc
2208 Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln
Leu 725 730 735 gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc
ccc gtg ctg 2256 Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly Pro Val Leu 740 745 750 ctg ccc gac aac cac tac ctg agc acc cag
tcc gcc ctg agc aaa gac 2304 Leu Pro Asp Asn His Tyr Leu Ser Thr
Gln Ser Ala Leu Ser Lys Asp 755 760 765 ccc aac gag aag cgc gat cac
atg gtc ctg ctg gag ttc gtg acc gcc 2352 Pro Asn Glu Lys Arg Asp
His Met Val Leu Leu Glu Phe Val Thr Ala 770 775 780 gcc ggg atc act
ctc ggc atg gac gag ctg tac ggt agc cga ggt gcg 2400 Ala Gly Ile
Thr Leu Gly Met Asp Glu Leu Tyr Gly Ser Arg Gly Ala 785 790 795 800
gct gat ggc acc aac tgg aaa atc gac aac aaa gtg gtc cgc gta cct
2448 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro 805 810 815 tat gtt ggc gta gat aaa gac aac ctg gct gaa ttc agc
aag aaa taa 2496 Tyr Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys 820 825 830 <210> SEQ ID NO 24 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-YFP-mglBF16A-G275S-CFP-K276R Vector <400> SEQUENCE: 24
Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5
10 15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys
Asp 20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr
Gly Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn
Gly His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly Glu Gly Asp Ala
Thr Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile Cys Thr Thr Gly
Lys Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val Thr Thr Leu Thr
Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105 110 His Met Lys
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 Val
Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135
140 Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu
145 150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu
Gly His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr
Ile Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile Lys Ala Asn Phe
Lys Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser Val Gln Leu Ala
Asp His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly Asp Gly Pro Val
Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230 235 240 Ser Ala
Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 245 250 255
Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260
265 270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val
Thr 275 280 285 Ile Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg
Lys Ala Ile 290 295 300 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln
Leu Leu Met Asn Asp 305 310 315 320 Ser Gln Asn Asp Gln Ser Lys Gln
Asn Asp Gln Ile Asp Val Leu Leu 325 330 335 Ala Lys Gly Val Lys Ala
Leu Ala Ile Asn Leu Val Asp Pro Ala Ala 340 345 350 Ala Gly Thr Val
Ile Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 Phe Phe
Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385
390 395 400 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp
Leu Asn 405 410 415 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu Lys Gly
Glu Pro Gly His 420 425 430 Pro Asp Ala Glu Ala Arg Thr Thr Tyr Val
Ile Lys Glu Leu Asn Asp 435 440 445 Lys Gly Ile Lys Thr Glu Gln Leu
Gln Leu Asp Thr Ala Met Trp Asp 450 455 460 Thr Ala Gln Ala Lys Asp
Lys Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470 475 480 Ala Asn Lys
Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met Ala Met 485 490 495 Gly
Ala Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val 500 505
510 Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly
515 520 525 Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala
Lys Ala 530 535 540 Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp Ser Ala
Gly Met Val Ser 545 550 555 560 Lys Gly Glu Glu Leu Phe Thr Gly Val
Val Pro Ile Leu Val Glu Leu 565 570 575 Asp Gly Asp Val Asn Gly His
Lys Phe Ser Val Ser Gly Glu Gly Glu 580 585 590 Gly Asp Ala Thr Tyr
Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 595 600 605 Gly Lys Leu
Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Trp 610 615 620 Gly
Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 625 630
635 640 Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr
Ile 645 650 655 Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu
Val Lys Phe 660 665 670 Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu
Lys Gly Ile Asp Phe 675 680 685 Lys Glu Asp Gly Asn Ile Leu Gly His
Lys Leu Glu Tyr Asn Tyr Ile 690 695 700 Ser His Asn Val Tyr Ile Thr
Ala Asp Lys Gln Lys Asn Gly Ile Lys 705 710 715 720 Ala Asn Phe Lys
Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 725 730 735 Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 740 745 750
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 755
760 765 Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr
Ala 770 775 780 Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Gly Ser
Arg Gly Ala 785 790 795 800 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn
Lys Val Val Arg Val Pro 805 810 815 Tyr Val Gly Val Asp Lys Asp Asn
Leu Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ ID NO 25
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-T282S-CFP-N283R Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 25 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tac gac gat aac gcg atg tct gta gtg cgc aag gct att 912 Ile
Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295
300 gag caa gat gcg aaa gcc gcg cca gat gtt cag ctg ctg atg aat gat
960 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp
305 310 315 320 tct cag aat gac cag tcc aag cag aac gat cag atc gac
gta ttg ctg 1008 Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile
Asp Val Leu Leu 325 330 335 gcg aaa ggg gtg aag gca ctg gca atc aac
ctg gtt gac ccg gca gct 1056 Ala Lys Gly Val Lys Ala Leu Ala Ile
Asn Leu Val Asp Pro Ala Ala 340 345 350 gcg ggt acg gtg att gag aaa
gcg cgt ggg caa aac gtg ccg gtg gtt 1104 Ala Gly Thr Val Ile Glu
Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 ttc ttc aac aaa
gaa ccg tct cgt aag gcg ctg gat agc tac gac aaa 1152 Phe Phe Asn
Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380 gcc
tac tac gtt ggc act gac tcc aaa gag tcc ggc att att caa ggc 1200
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385
390 395 400 gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
ctg aac 1248 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp Leu Asn 405 410 415 aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg ggc cat 1296 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro Gly His 420 425 430 ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg aac gat 1344 Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu Asn Asp 435 440 445 aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg tgg gac 1392 Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp 450 455 460 acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc ccg aac 1440 Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470
475 480 gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg gca
atg 1488 Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met
Ala Met 485 490 495 ggc gcg gtt gaa gcg ctg aaa gca cac aac aag tcc
agc att ccg gtg 1536 Gly Ala Val Glu Ala Leu Lys Ala His Asn Lys
Ser Ser Ile Pro Val 500 505 510 ttt ggc gtc gat gcg ctg cca gaa gcg
ctg gcg ctg gtg aaa tcc ggt 1584 Phe Gly Val Asp Ala Leu Pro Glu
Ala Leu Ala Leu Val Lys Ser Gly 515 520 525 gca ctg gcg ggc acc gta
ctg aac gat gct aac aac cag gcg aaa gcg 1632 Ala Leu Ala Gly Thr
Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala 530 535 540 acc ttt gat
ctg gcg aaa aac ctg gcc gat ggt aaa ggt gcg gct gat 1680 Thr Phe
Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala Ala Asp 545 550 555
560 ggc tcg gct ggt atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg
1728 Gly Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val 565 570 575 gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc
cac aag ttc 1776 Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn
Gly His Lys Phe 580 585 590 agc gtg tcc ggc gag ggc gag ggc gat gcc
acc tac ggc aag ctg acc 1824 Ser Val Ser Gly Glu Gly Glu Gly Asp
Ala Thr Tyr Gly Lys Leu Thr 595 600 605 ctg aag ttc atc tgc acc acc
ggc aag ctg ccc gtg ccc tgg ccc acc 1872 Leu Lys Phe Ile Cys Thr
Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 610 615 620 ctc gtg acc acc
ctg acc tgg ggc gtg cag tgc ttc agc cgc tac ccc 1920 Leu Val Thr
Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro 625 630 635 640
gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc
1968 Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu
Gly 645 650 655 tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc
aac tac aag 2016 Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys 660 665 670 acc cgc gcc gag gtg aag ttc gag ggc gac
acc ctg gtg aac cgc atc 2064 Thr Arg Ala Glu Val Lys Phe Glu Gly
Asp Thr Leu Val Asn Arg Ile 675 680 685 gag ctg aag ggc atc gac ttc
aag gag gac ggc aac atc ctg ggg cac 2112 Glu Leu Lys Gly Ile Asp
Phe Lys Glu Asp Gly Asn Ile Leu Gly His 690 695 700 aag ctg gag tac
aac tac atc agc cac aac gtc tat atc acc gcc gac 2160 Lys Leu Glu
Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp 705 710 715 720
aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc
2208 Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile 725 730 735 gag gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc 2256 Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln
Gln Asn Thr Pro 740 745 750 atc ggc gac ggc ccc gtg ctg ctg ccc gac
aac cac tac ctg agc acc 2304 Ile Gly Asp Gly Pro Val Leu Leu Pro
Asp Asn His Tyr Leu Ser Thr 755 760 765 cag tcc gcc ctg agc aaa gac
ccc aac gag aag cgc gat cac atg gtc 2352 Gln Ser Ala Leu Ser Lys
Asp Pro Asn Glu Lys Arg Asp His Met Val 770 775 780 ctg ctg gag ttc
gtg acc gcc gcc ggg atc act ctc ggc atg gac gag 2400 Leu Leu Glu
Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu 785 790 795 800
ctg tac ggt agc cga tgg aaa atc gac aac aaa gtg gtc cgc gta cct
2448 Leu Tyr Gly Ser Arg Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro 805 810 815 tat gtt ggc gta gat aaa gac aac ctg gct gaa ttc agc
aag aaa taa 2496 Tyr Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys 820 825 830 <210> SEQ ID NO 26 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-YFP-mglBF16A-T282S-CFP-N283R Vector <400> SEQUENCE: 26
Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5
10 15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys
Asp 20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr
Gly Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn
Gly His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly Glu Gly Asp Ala
Thr Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile Cys Thr Thr Gly
Lys Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val Thr Thr Leu Thr
Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105 110 His Met Lys
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 Val
Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135
140 Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu
145 150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu
Gly His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr
Ile Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile Lys Ala Asn Phe
Lys Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser Val Gln Leu Ala
Asp His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly Asp Gly Pro Val
Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230 235 240 Ser Ala
Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 245 250 255
Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260
265 270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val
Thr 275 280 285 Ile Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg
Lys Ala Ile 290 295 300 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln
Leu Leu Met Asn Asp 305 310 315 320 Ser Gln Asn Asp Gln Ser Lys Gln
Asn Asp Gln Ile Asp Val Leu Leu 325 330 335 Ala Lys Gly Val Lys Ala
Leu Ala Ile Asn Leu Val Asp Pro Ala Ala 340 345 350 Ala Gly Thr Val
Ile Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 Phe Phe
Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385
390 395 400 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp
Leu Asn 405 410 415 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu Lys Gly
Glu Pro Gly His 420 425 430 Pro Asp Ala Glu Ala Arg Thr Thr Tyr Val
Ile Lys Glu Leu Asn Asp 435 440 445 Lys Gly Ile Lys Thr Glu Gln Leu
Gln Leu Asp Thr Ala Met Trp Asp 450 455 460 Thr Ala Gln Ala Lys Asp
Lys Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470 475 480 Ala Asn Lys
Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met Ala Met 485 490 495 Gly
Ala Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val 500 505
510 Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly
515 520 525 Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala
Lys Ala 530 535 540 Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys
Gly Ala Ala Asp 545 550 555 560 Gly Ser Ala Gly Met Val Ser Lys Gly
Glu Glu Leu Phe Thr Gly Val 565 570 575 Val Pro Ile Leu Val Glu Leu
Asp Gly Asp Val Asn Gly His Lys Phe 580 585 590 Ser Val Ser Gly Glu
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr 595 600 605 Leu Lys Phe
Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 610 615 620 Leu
Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro 625 630
635 640 Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu
Gly 645 650 655 Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly
Asn Tyr Lys 660 665 670 Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr
Leu Val Asn Arg Ile 675 680 685 Glu Leu Lys Gly Ile Asp Phe Lys Glu
Asp Gly Asn Ile Leu Gly His 690 695 700 Lys Leu Glu Tyr Asn Tyr Ile
Ser His Asn Val Tyr Ile Thr Ala Asp 705 710 715 720 Lys Gln Lys Asn
Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile 725 730 735 Glu Asp
Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 740 745 750
Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr 755
760 765 Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met
Val 770 775 780 Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly
Met Asp Glu 785 790 795 800 Leu Tyr Gly Ser Arg Trp Lys Ile Asp Asn
Lys Val Val Arg Val Pro 805 810 815 Tyr Val Gly Val Asp Lys Asp Asn
Leu Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ ID NO 27
<211> LENGTH: 909 <212> TYPE: DNA <213> ORGANISM:
Escherichia coli K12 <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(909) <400> SEQUENCE: 27 atg caa
tta cgt aaa cct gcc aca gca atc ctc gcc ctg gcg ctt tcc 48 Met Gln
Leu Arg Lys Pro Ala Thr Ala Ile Leu Ala Leu Ala Leu Ser 1 5 10 15
gca gga ctg gca cag gca gat gac gcc gcc ccg gca gcg ggc agt act 96
Ala Gly Leu Ala Gln Ala Asp Asp Ala Ala Pro Ala Ala Gly Ser Thr 20
25 30 ctg gac aaa atc gcc aaa aac ggt gtg att gtc gtc ggt cac cgt
gaa 144 Leu Asp Lys Ile Ala Lys Asn Gly Val Ile Val Val Gly His Arg
Glu 35 40 45 tct tca gtg cct ttc tct tat tac gac aat cag caa aaa
gtg gtg ggt 192 Ser Ser Val Pro Phe Ser Tyr Tyr Asp Asn Gln Gln Lys
Val Val Gly 50 55 60 tac tcg cag gat tac tcc aac gcc att gtt gaa
gca gtg aaa aag aaa 240 Tyr Ser Gln Asp Tyr Ser Asn Ala Ile Val Glu
Ala Val Lys Lys Lys 65 70 75 80 ctc aac aaa ccg gac ttg cag gta aaa
ctg att ccg att acc tca caa 288 Leu Asn Lys Pro Asp Leu Gln Val Lys
Leu Ile Pro Ile Thr Ser Gln 85 90 95 aac cgt att cca ctg ctg caa
aac ggc act ttc gat ttt gaa tgt ggt 336 Asn Arg Ile Pro Leu Leu Gln
Asn Gly Thr Phe Asp Phe Glu Cys Gly 100 105 110 tct acc acc aac aac
gtc gaa cgc caa aaa cag gcg gct ttc tct gac 384 Ser Thr Thr Asn Asn
Val Glu Arg Gln Lys Gln Ala Ala Phe Ser Asp 115 120 125 act att ttc
gtg gtc ggt acg cgc ctg ttg acc aaa aag ggt ggc gat 432 Thr Ile Phe
Val Val Gly Thr Arg Leu Leu Thr Lys Lys Gly Gly Asp 130 135 140 atc
aaa gat ttt gcc aac ctg aaa gac aaa gcc gta gtc gtc act tcc 480 Ile
Lys Asp Phe Ala Asn Leu Lys Asp Lys Ala Val Val Val Thr Ser 145 150
155 160 ggc act acc tct gaa gtt ttg ctc aac aaa ctg aat gaa gag caa
aaa 528 Gly Thr Thr Ser Glu Val Leu Leu Asn Lys Leu Asn Glu Glu Gln
Lys 165 170 175 atg aat atg cgc atc atc agc gcc aaa gat cac ggt gac
tct ttc cgc 576 Met Asn Met Arg Ile Ile Ser Ala Lys Asp His Gly Asp
Ser Phe Arg 180 185 190 acc ctg gaa agc ggt cgt gcc gtt gcc ttt atg
atg gat gac gct ctg 624 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe Met
Met Asp Asp Ala Leu 195 200 205 ctg gcc ggt gaa cgt gcg aaa gcg aag
aaa cca gac aac tgg gaa atc 672 Leu Ala Gly Glu Arg Ala Lys Ala Lys
Lys Pro Asp Asn Trp Glu Ile 210 215 220 gtc ggc aag ccg cag tct cag
gag gcc tac ggt tgt atg ttg cgt aaa 720 Val Gly Lys Pro Gln Ser Gln
Glu Ala Tyr Gly Cys Met Leu Arg Lys 225 230 235 240 gat gat ccg cag
ttc aaa aag ctg atg gat gac acc atc gct cag gtg 768 Asp Asp Pro Gln
Phe Lys Lys Leu Met Asp Asp Thr Ile Ala Gln Val 245 250 255 cag acc
tcc ggt gaa gcg gaa aaa tgg ttt gat aag tgg ttc aaa aat 816 Gln Thr
Ser Gly Glu Ala Glu Lys Trp Phe Asp Lys Trp Phe Lys Asn 260 265 270
cca att ccg ccg aaa aac ctg aac atg aat ttc gaa ctg tca gac gaa 864
Pro Ile Pro Pro Lys Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu 275
280 285 atg aaa gca ctg ttc aaa gaa ccg aat gac aag gca ctg aac taa
909 Met Lys Ala Leu Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn 290 295
300 <210> SEQ ID NO 28 <211> LENGTH: 302 <212>
TYPE: PRT <213> ORGANISM: Escherichia coli K12 <400>
SEQUENCE: 28 Met Gln Leu Arg Lys Pro Ala Thr Ala Ile Leu Ala Leu
Ala Leu Ser 1 5 10 15 Ala Gly Leu Ala Gln Ala Asp Asp Ala Ala Pro
Ala Ala Gly Ser Thr 20 25 30 Leu Asp Lys Ile Ala Lys Asn Gly Val
Ile Val Val Gly His Arg Glu 35 40 45 Ser Ser Val Pro Phe Ser Tyr
Tyr Asp Asn Gln Gln Lys Val Val Gly 50 55 60 Tyr Ser Gln Asp Tyr
Ser Asn Ala Ile Val Glu Ala Val Lys Lys Lys 65 70 75 80 Leu Asn Lys
Pro Asp Leu Gln Val Lys Leu Ile Pro Ile Thr Ser Gln 85 90 95 Asn
Arg Ile Pro Leu Leu Gln Asn Gly Thr Phe Asp Phe Glu Cys Gly 100 105
110 Ser Thr Thr Asn Asn Val Glu Arg Gln Lys Gln Ala Ala Phe Ser Asp
115 120 125 Thr Ile Phe Val Val Gly Thr Arg Leu Leu Thr Lys Lys Gly
Gly Asp 130 135 140 Ile Lys Asp Phe Ala Asn Leu Lys Asp Lys Ala Val
Val Val Thr Ser 145 150 155 160 Gly Thr Thr Ser Glu Val Leu Leu Asn
Lys Leu Asn Glu Glu Gln Lys 165 170 175 Met Asn Met Arg Ile Ile Ser
Ala Lys Asp His Gly Asp Ser Phe Arg 180 185 190 Thr Leu Glu Ser Gly
Arg Ala Val Ala Phe Met Met Asp Asp Ala Leu 195 200 205 Leu Ala Gly
Glu Arg Ala Lys Ala Lys Lys Pro Asp Asn Trp Glu Ile 210 215 220 Val
Gly Lys Pro Gln Ser Gln Glu Ala Tyr Gly Cys Met Leu Arg Lys 225 230
235 240 Asp Asp Pro Gln Phe Lys Lys Leu Met Asp Asp Thr Ile Ala Gln
Val 245 250 255 Gln Thr Ser Gly Glu Ala Glu Lys Trp Phe Asp Lys Trp
Phe Lys Asn 260 265 270 Pro Ile Pro Pro Lys Asn Leu Asn Met Asn Phe
Glu Leu Ser Asp Glu 275 280 285 Met Lys Ala Leu Phe Lys Glu Pro Asn
Asp Lys Ala Leu Asn 290 295 300 <210> SEQ ID NO 29
<400> SEQUENCE: 29 000 <210> SEQ ID NO 30 <211>
LENGTH: 6 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: linker
sequence <400> SEQUENCE: 30 Gly Asn Asn Ser Ala Gly 1 5
<210> SEQ ID NO 31 <211> LENGTH: 6 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: linker sequence <400>
SEQUENCE: 31 Gly Ser Ala Asp Asp Gly 1 5 <210> SEQ ID NO 32
<211> LENGTH: 6 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: linker sequence <400> SEQUENCE: 32 Gly Gly Thr
Gly Gly Ala 1 5 <210> SEQ ID NO 33 <211> LENGTH: 18
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: linker sequence
<400> SEQUENCE: 33 ggtggtaccg gaggcgcc 18 <210> SEQ ID
NO 34 <211> LENGTH: 15 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 34 agcgctggta tggtg 15 <210> SEQ ID NO
35 <211> LENGTH: 15 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 35 actctcggta gcgct 15 <210> SEQ ID NO
36 <211> LENGTH: 4 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 36 Ala Gly Met Val 1 <210> SEQ ID NO 37
<211> LENGTH: 4 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 37 Thr Leu Gly Ser 1 <210> SEQ ID NO 38
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB A216-cpVenus-K217
<400> SEQUENCE: 38 aaagcgggca acaacagcgc tggtgacggc 30
<210> SEQ ID NO 39 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from pRSETB
A216-cpVenus-K217 <400> SEQUENCE: 39 atcgagggta gcgctgacga
cggaaagaaa 30 <210> SEQ ID NO 40 <211> LENGTH: 10
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: partial
sequence from pRSETB A216-cpVenus-K217 <400> SEQUENCE: 40 Lys
Ala Gly Asn Asn Ser Ala Gly Asn Gly 1 5 10 <210> SEQ ID NO 41
<211> LENGTH: 10 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB A216-cpVenus-K217
<400> SEQUENCE: 41 Ile Glu Gly Ser Ala Asp Asp Gly Lys Lys 1
5 10 <210> SEQ ID NO 42 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from
FLII275 Pglu plasmid <400> SEQUENCE: 42 actctcggca tggacgagct
gtacaagggt ggtaccggag gcgcc 45 <210> SEQ ID NO 43 <211>
LENGTH: 33 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
partial sequence from FLII2 Pglu plasmid <400> SEQUENCE: 43
actctcggca tggacgagct gtacggtagc cga 33 <210> SEQ ID NO 44
<211> LENGTH: 51 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 44 gaattcagca agaaaggcgc cggtaccggt ggaatggtga gcaagggcga
g 51 <210> SEQ ID NO 45 <211> LENGTH: 39 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from FLII2
Pglu plasmid <400> SEQUENCE: 45 gaattcagca agaaaggtac
catggtgagc aagggcgag 39 <210> SEQ ID NO 46 <211>
LENGTH: 33 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
partial sequence from FLII2 Pglu plasmid <400> SEQUENCE: 46
gaattcagca agaaaatggt gagcaagggc gag 33 <210> SEQ ID NO 47
<211> LENGTH: 48 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 47 ttcagcaaga aaggcgccgg taccggtgga atggtgagca agggcgag
48 <210> SEQ ID NO 48 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from FLII2
Pglu plasmid <400> SEQUENCE: 48 agcaagaaag gcgccggtac
cggtggaatg gtgagcaagg gcgag 45 <210> SEQ ID NO 49 <211>
LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
partial sequence from FLII2 Pglu plasmid <400> SEQUENCE: 49
aagaaaggcg ccggtaccgg tggaatggtg agcaagggcg ag 42 <210> SEQ
ID NO 50 <211> LENGTH: 39 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from FLII2 Pglu plasmid
<400> SEQUENCE: 50 aaaggcgccg gtaccggtgg aatggtgagc aagggcgag
39 <210> SEQ ID NO 51 <211> LENGTH: 36 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from FLII2
Pglu plasmid <400> SEQUENCE: 51 ggcgccggta ccggtggaat
ggtgagcaag ggcgag 36 <210> SEQ ID NO 52 <211> LENGTH:
33 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: partial
sequence from FLII2 Pglu plasmid <400> SEQUENCE: 52
gccggtaccg gtggaatggt gagcaagggc gag 33 <210> SEQ ID NO 53
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 53 ggtaccggtg gaatggtgag caagggcgag 30 <210> SEQ ID
NO 54 <211> LENGTH: 27 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from FLII2 Pglu plasmid
<400> SEQUENCE: 54 accggtggaa tggtgagcaa gggcgag 27
<210> SEQ ID NO 55 <211> LENGTH: 24 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 55 ggtggaatgg tgagcaaggg cgag 24
<210> SEQ ID NO 56 <211> LENGTH: 21 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 56 ggaatggtga gcaagggcga g 21
<210> SEQ ID NO 57 <211> LENGTH: 18 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 57 atggtgagca agggcgag 18 <210>
SEQ ID NO 58 <211> LENGTH: 15 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 58 gtgagcaagg gcgag 15 <210>
SEQ ID NO 59 <211> LENGTH: 12 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 59 agcaagggcg ag 12
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 59 <210>
SEQ ID NO 1 <211> LENGTH: 978 <212> TYPE: DNA
<213> ORGANISM: Escherichia coli K12 <220> FEATURE:
<221> NAME/KEY: CDS <222> LOCATION: (1)..(978)
<400> SEQUENCE: 1 atg ata aca aca caa aca ctc aca acg ggt atc
cat gcg ttc tta acg 48 Met Ile Thr Thr Gln Thr Leu Thr Thr Gly Ile
His Ala Phe Leu Thr 1 5 10 15 cag aag ata aag gag ttg gat atg caa
tta cgt aaa cct gcc aca gca 96 Gln Lys Ile Lys Glu Leu Asp Met Gln
Leu Arg Lys Pro Ala Thr Ala 20 25 30 atc ctc gcc ctg gcg ctt tcc
gca gga ctg gca cag gca gat gac gcc 144 Ile Leu Ala Leu Ala Leu Ser
Ala Gly Leu Ala Gln Ala Asp Asp Ala 35 40 45 gcc ccg gca gcg ggc
agt act ctg gac aaa atc gcc aaa aac ggt gtg 192 Ala Pro Ala Ala Gly
Ser Thr Leu Asp Lys Ile Ala Lys Asn Gly Val 50 55 60 att gtc gtc
ggt cac cgt gaa tct tca gtg cct ttc tct tat tac gac 240 Ile Val Val
Gly His Arg Glu Ser Ser Val Pro Phe Ser Tyr Tyr Asp 65 70 75 80 aat
cag caa aaa gtg gtg ggt tac tcg cag gat tac tcc aac gcc att 288 Asn
Gln Gln Lys Val Val Gly Tyr Ser Gln Asp Tyr Ser Asn Ala Ile 85 90
95 gtt gaa gca gtg aaa aag aaa ctc aac aaa ccg gac ttg cag gta aaa
336 Val Glu Ala Val Lys Lys Lys Leu Asn Lys Pro Asp Leu Gln Val Lys
100 105 110 ctg att ccg att acc tca caa aac cgt att cca ctg ctg caa
aac ggc 384 Leu Ile Pro Ile Thr Ser Gln Asn Arg Ile Pro Leu Leu Gln
Asn Gly 115 120 125 act ttc gat ttt gaa tgt ggt tct acc acc aac aac
gtc gaa cgc caa 432 Thr Phe Asp Phe Glu Cys Gly Ser Thr Thr Asn Asn
Val Glu Arg Gln 130 135 140 aaa cag gcg gct ttc tct gac act att ttc
gtg gtc ggt acg cgc ctg 480 Lys Gln Ala Ala Phe Ser Asp Thr Ile Phe
Val Val Gly Thr Arg Leu 145 150 155 160 ttg acc aaa aag ggt ggc gat
atc aaa gat ttt gcc aac ctg aaa gac 528 Leu Thr Lys Lys Gly Gly Asp
Ile Lys Asp Phe Ala Asn Leu Lys Asp 165 170 175 aaa gcc gta gtc gtc
act tcc ggc act acc tct gaa gtt ttg ctc aac 576 Lys Ala Val Val Val
Thr Ser Gly Thr Thr Ser Glu Val Leu Leu Asn 180 185 190 aaa ctg aat
gaa gag caa aaa atg aat atg cgc atc atc agc gcc aaa 624 Lys Leu Asn
Glu Glu Gln Lys Met Asn Met Arg Ile Ile Ser Ala Lys 195 200 205 gat
cac ggt gac tct ttc cgc acc ctg gaa agc ggt cgt gcc gtt gcc 672 Asp
His Gly Asp Ser Phe Arg Thr Leu Glu Ser Gly Arg Ala Val Ala 210 215
220 ttt atg atg gat gac gct ctg ctg gcc ggt gaa cgt gcg aaa gcg aag
720 Phe Met Met Asp Asp Ala Leu Leu Ala Gly Glu Arg Ala Lys Ala Lys
225 230 235 240 aaa cca gac aac tgg gaa atc gtc ggc aag ccg cag tct
cag gag gcc 768 Lys Pro Asp Asn Trp Glu Ile Val Gly Lys Pro Gln Ser
Gln Glu Ala 245 250 255 tac ggt tgt atg ttg cgt aaa gat gat ccg cag
ttc aaa aag ctg atg 816 Tyr Gly Cys Met Leu Arg Lys Asp Asp Pro Gln
Phe Lys Lys Leu Met 260 265 270 gat gac acc atc gct cag gtg cag acc
tcc ggt gaa gcg gaa aaa tgg 864 Asp Asp Thr Ile Ala Gln Val Gln Thr
Ser Gly Glu Ala Glu Lys Trp 275 280 285 ttt gat aag tgg ttc aaa aat
cca att ccg ccg aaa aac ctg aac atg 912 Phe Asp Lys Trp Phe Lys Asn
Pro Ile Pro Pro Lys Asn Leu Asn Met 290 295 300 aat ttc gaa ctg tca
gac gaa atg aaa gca ctg ttc aaa gaa ccg aat 960 Asn Phe Glu Leu Ser
Asp Glu Met Lys Ala Leu Phe Lys Glu Pro Asn 305 310 315 320 gac aag
gca ctg aac taa 978 Asp Lys Ala Leu Asn 325 <210> SEQ ID NO 2
<211> LENGTH: 325 <212> TYPE: PRT <213> ORGANISM:
Escherichia coli K12 <400> SEQUENCE: 2 Met Ile Thr Thr Gln
Thr Leu Thr Thr Gly Ile His Ala Phe Leu Thr 1 5 10 15 Gln Lys Ile
Lys Glu Leu Asp Met Gln Leu Arg Lys Pro Ala Thr Ala 20 25 30 Ile
Leu Ala Leu Ala Leu Ser Ala Gly Leu Ala Gln Ala Asp Asp Ala 35 40
45 Ala Pro Ala Ala Gly Ser Thr Leu Asp Lys Ile Ala Lys Asn Gly Val
50 55 60 Ile Val Val Gly His Arg Glu Ser Ser Val Pro Phe Ser Tyr
Tyr Asp 65 70 75 80 Asn Gln Gln Lys Val Val Gly Tyr Ser Gln Asp Tyr
Ser Asn Ala Ile 85 90 95 Val Glu Ala Val Lys Lys Lys Leu Asn Lys
Pro Asp Leu Gln Val Lys 100 105 110 Leu Ile Pro Ile Thr Ser Gln Asn
Arg Ile Pro Leu Leu Gln Asn Gly 115 120 125 Thr Phe Asp Phe Glu Cys
Gly Ser Thr Thr Asn Asn Val Glu Arg Gln 130 135 140 Lys Gln Ala Ala
Phe Ser Asp Thr Ile Phe Val Val Gly Thr Arg Leu 145 150 155 160 Leu
Thr Lys Lys Gly Gly Asp Ile Lys Asp Phe Ala Asn Leu Lys Asp 165 170
175 Lys Ala Val Val Val Thr Ser Gly Thr Thr Ser Glu Val Leu Leu Asn
180 185 190 Lys Leu Asn Glu Glu Gln Lys Met Asn Met Arg Ile Ile Ser
Ala Lys 195 200 205 Asp His Gly Asp Ser Phe Arg Thr Leu Glu Ser Gly
Arg Ala Val Ala 210 215 220 Phe Met Met Asp Asp Ala Leu Leu Ala Gly
Glu Arg Ala Lys Ala Lys 225 230 235 240 Lys Pro Asp Asn Trp Glu Ile
Val Gly Lys Pro Gln Ser Gln Glu Ala 245 250 255 Tyr Gly Cys Met Leu
Arg Lys Asp Asp Pro Gln Phe Lys Lys Leu Met 260 265 270 Asp Asp Thr
Ile Ala Gln Val Gln Thr Ser Gly Glu Ala Glu Lys Trp 275 280 285 Phe
Asp Lys Trp Phe Lys Asn Pro Ile Pro Pro Lys Asn Leu Asn Met 290 295
300 Asn Phe Glu Leu Ser Asp Glu Met Lys Ala Leu Phe Lys Glu Pro Asn
305 310 315 320 Asp Lys Ala Leu Asn 325 <210> SEQ ID NO 3
<211> LENGTH: 810 <212> TYPE: DNA <213> ORGANISM:
Escherichia coli K12 <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(810) <400> SEQUENCE: 3 ctg gac
aaa atc gcc aaa aac ggt gtg att gtc gtc ggt cac cgt gaa 48 Leu Asp
Lys Ile Ala Lys Asn Gly Val Ile Val Val Gly His Arg Glu 1 5 10 15
tct tca gtg cct ttc tct tat tac gac aat cag caa aaa gtg gtg ggt 96
Ser Ser Val Pro Phe Ser Tyr Tyr Asp Asn Gln Gln Lys Val Val Gly 20
25 30 tac tcg cag gat tac tcc aac gcc att gtt gaa gca gtg aaa aag
aaa 144 Tyr Ser Gln Asp Tyr Ser Asn Ala Ile Val Glu Ala Val Lys Lys
Lys 35 40 45 ctc aac aaa ccg gac ttg cag gta aaa ctg att ccg att
acc tca caa 192 Leu Asn Lys Pro Asp Leu Gln Val Lys Leu Ile Pro Ile
Thr Ser Gln 50 55 60 aac cgt att cca ctg ctg caa aac ggc act ttc
gat ttt gaa tgt ggt 240 Asn Arg Ile Pro Leu Leu Gln Asn Gly Thr Phe
Asp Phe Glu Cys Gly 65 70 75 80 tct acc acc aac aac gtc gaa cgc caa
aaa cag gcg gct ttc tct gac 288 Ser Thr Thr Asn Asn Val Glu Arg Gln
Lys Gln Ala Ala Phe Ser Asp 85 90 95 act att ttc gtg gtc ggt acg
cgc ctg ttg acc aaa aag ggt ggc gat 336 Thr Ile Phe Val Val Gly Thr
Arg Leu Leu Thr Lys Lys Gly Gly Asp 100 105 110 atc aaa gat ttt gcc
aac ctg aaa gac aaa gcc gta gtc gtc act tcc 384 Ile Lys Asp Phe Ala
Asn Leu Lys Asp Lys Ala Val Val Val Thr Ser 115 120 125 ggc act acc
tct gaa gtt ttg ctc aac aaa ctg aat gaa gag caa aaa 432 Gly Thr Thr
Ser Glu Val Leu Leu Asn Lys Leu Asn Glu Glu Gln Lys 130 135 140 atg
aat atg cgc atc atc agc gcc aaa gat cac ggt gac tct ttc cgc 480 Met
Asn Met Arg Ile Ile Ser Ala Lys Asp His Gly Asp Ser Phe Arg 145 150
155 160 acc ctg gaa agc ggt cgt gcc gtt gcc ttt atg atg gat gac gct
ctg 528 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe Met Met Asp Asp Ala
Leu 165 170 175 ctg gcc ggt gaa cgt gcg aaa gcg aag aaa cca gac aac
tgg gaa atc 576 Leu Ala Gly Glu Arg Ala Lys Ala Lys Lys Pro Asp Asn
Trp Glu Ile 180 185 190 gtc ggc aag ccg cag tct cag gag gcc tac ggt
tgt atg ttg cgt aaa 624 Val Gly Lys Pro Gln Ser Gln Glu Ala Tyr Gly
Cys Met Leu Arg Lys 195 200 205 gat gat ccg cag ttc aaa aag ctg atg
gat gac acc atc gct cag gtg 672 Asp Asp Pro Gln Phe Lys Lys Leu Met
Asp Asp Thr Ile Ala Gln Val 210 215 220 cag acc tcc ggt gaa gcg gaa
aaa tgg ttt gat aag tgg ttc aaa aat 720 Gln Thr Ser Gly Glu Ala Glu
Lys Trp Phe Asp Lys Trp Phe Lys Asn 225 230 235 240 cca att ccg ccg
aaa aac ctg aac atg aat ttc gaa ctg tca gac gaa 768 Pro Ile Pro Pro
Lys Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu 245 250 255 atg aaa
gca ctg ttc aaa gaa ccg aat gac aag gca ctg aac 810 Met Lys Ala Leu
Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn 260 265 270
<210> SEQ ID NO 4 <211> LENGTH: 270 <212> TYPE:
PRT <213> ORGANISM: Escherichia coli K12 <400>
SEQUENCE: 4 Leu Asp Lys Ile Ala Lys Asn Gly Val Ile Val Val Gly His
Arg Glu 1 5 10 15 Ser Ser Val Pro Phe Ser Tyr Tyr Asp Asn Gln Gln
Lys Val Val Gly 20 25 30 Tyr Ser Gln Asp Tyr Ser Asn Ala Ile Val
Glu Ala Val Lys Lys Lys 35 40 45 Leu Asn Lys Pro Asp Leu Gln Val
Lys Leu Ile Pro Ile Thr Ser Gln 50 55 60 Asn Arg Ile Pro Leu Leu
Gln Asn Gly Thr Phe Asp Phe Glu Cys Gly 65 70 75 80 Ser Thr Thr Asn
Asn Val Glu Arg Gln Lys Gln Ala Ala Phe Ser Asp 85 90 95 Thr Ile
Phe Val Val Gly Thr Arg Leu Leu Thr Lys Lys Gly Gly Asp 100 105 110
Ile Lys Asp Phe Ala Asn Leu Lys Asp Lys Ala Val Val Val Thr Ser 115
120 125 Gly Thr Thr Ser Glu Val Leu Leu Asn Lys Leu Asn Glu Glu Gln
Lys 130 135 140 Met Asn Met Arg Ile Ile Ser Ala Lys Asp His Gly Asp
Ser Phe Arg 145 150 155 160 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe
Met Met Asp Asp Ala Leu 165 170 175 Leu Ala Gly Glu Arg Ala Lys Ala
Lys Lys Pro Asp Asn Trp Glu Ile 180 185 190 Val Gly Lys Pro Gln Ser
Gln Glu Ala Tyr Gly Cys Met Leu Arg Lys 195 200 205 Asp Asp Pro Gln
Phe Lys Lys Leu Met Asp Asp Thr Ile Ala Gln Val 210 215 220 Gln Thr
Ser Gly Glu Ala Glu Lys Trp Phe Asp Lys Trp Phe Lys Asn 225 230 235
240 Pro Ile Pro Pro Lys Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu
245 250 255 Met Lys Ala Leu Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn
260 265 270 <210> SEQ ID NO 5 <211> LENGTH: 39
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer
<400> SEQUENCE: 5 ggtaccggag gcgccgcagg cagcacgctg gacaaaatc
39 <210> SEQ ID NO 6 <211> LENGTH: 39 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer <400> SEQUENCE: 6
accggtaccg gcgccgttca gtgccttgtc attcggttc 39 <210> SEQ ID NO
7 <211> LENGTH: 30 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer <400> SEQUENCE: 7 gagcccggga
tggtgagcaa gggcgaggag 30 <210> SEQ ID NO 8 <211>
LENGTH: 33 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
<400> SEQUENCE: 8 gaggtcgacc ttgtacagct cgtccatgcc gag 33
<210> SEQ ID NO 9 <211> LENGTH: 5206 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: DNA sequence of pRSETB FLIP-E 600n
vector <400> SEQUENCE: 9 atctcgatcc cgcgaaatta atacgactca
ctatagggag accacaacgg tttccctcta 60 gataattttg tttaacttta
agaaggagat atacatatgc ggggttctca tcatcatcat 120 catcatggta
tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat 180
gacgataagg atccgggccg catggtgagc aagggcgagg agctgttcac cggggtggtg
240 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
gtccggcgag 300 ggcgagggcg atgccaccta cggcaagctg accctgaagt
tcatctgcac caccggcaag 360 ctgcccgtgc cctggcccac cctcgtgacc
accctgacct ggggcgtgca gtgcttcagc 420 cgctaccccg accacatgaa
gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 480 gtccaggagc
gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 540
aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
600 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
cgtctatatc 660 accgccgaca agcagaagaa cggcatcaag gccaacttca
agatccgcca caacatcgag 720 gacggcagcg tgcagctcgc cgaccactac
cagcagaaca cccccatcgg cgacggcccc 780 gtgctgctgc ccgacaacca
ctacctgagc acccagtccg ccctgagcaa agaccccaac 840 gagaagcgcg
atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 900
atggacgagc tgtacaaggg tggtaccgga ggcgccgcag gcagcacgct ggacaaaatc
960 gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt cagtgccttt
ctcttattac 1020 gacaatcagc aaaaagtggt gggttactcg caggattact
ccaacgccat tgttgaagca 1080 gtgaaaaaga aactcaacaa accggacttg
caggtaaaac tgattccgat tacctcacaa 1140 aaccgtattc cactgctgca
aaacggcact ttcgattttg aatgtggttc taccaccaac 1200 aacgtcgaac
gccaaaaaca ggcggctttc tctgacacta ttttcgtggt cggtacgcgc 1260
ctgttgacca aaaagggtgg cgatatcaaa gattttgcca acctgaaaga caaagccgta
1320 gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca aactgaatga
agagcaaaaa 1380 atgaatatgc gcatcatcag cgccaaagat cacggtgact
ctttccgcac cctggaaagc 1440 ggtcgtgccg ttgcctttat gatggatgac
gctctgctgg ccggtgaacg tgcgaaagcg 1500 aagaaaccag acaactggga
aatcgtcggc aagccgcagt ctcaggaggc ctacggttgt 1560 atgttgcgta
aagatgatcc gcagttcaaa aagctgatgg atgacaccat cgctcaggtg 1620
cagacctccg gtgaagcgga aaaatggttt gataagtggt tcaaaaatcc aattccgccg
1680 aaaaacctga acatgaattt cgaactgtca gacgaaatga aagcactgtt
caaagaaccg 1740 aatgacaagg cactgaacgg cgccggtacc ggtggaatgg
tgagcaaggg cgaggagctg 1800 ttcaccgggg tggtgcccat cctggtcgag
ctggacggcg acgtaaacgg ccacaagttc 1860 agcgtgtccg gcgagggcga
gggcgatgcc acctacggca agctgaccct gaagttcatc 1920 tgcaccaccg
gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc 1980
ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
2040 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
caactacaag 2100 acccgcgccg aggtgaagtt cgagggcgac accctggtga
accgcatcga gctgaagggc 2160 atcgacttca aggaggacgg caacatcctg
gggcacaagc tggagtacaa ctacaacagc 2220 cacaacgtct atatcatggc
cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 2280 cgccacaaca
tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2340
atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
2400 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
gaccgccgcc 2460 gggatcactc tcggcatgga cgagctgtac aagtaaaagc
ttgatccggc tgctaacaaa 2520 gcccgaaagg aagctgagtt ggctgctgcc
accgctgagc aataactagc ataacccctt 2580 ggggcctcta aacgggtctt
gaggggtttt ttgctgaaag gaggaactat atccggatct 2640 ggcgtaatag
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 2700
gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
2760 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
ttcccttcct 2820 ttctcgccac gttcgccggc tttccccgtc aagctctaaa
tcgggggctc cctttagggt 2880 tccgatttag agctttacgg cacctcgacc
gcaaaaaact tgatttgggt gatggttcac 2940 gtagtgggcc atcgccctga
tagacggttt ttcgcccttt gacgttggag tccacgttct 3000 ttaatagtgg
actcttgttc caaactggaa caacactcaa ccctatcgcg gtctattctt 3060
ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3120 aaatatttaa cgcgaatttt aacaaaatat taacgtttac aatttcgcct
gatgcggtat 3180 tttctcctta cgcatctgtg cggtatttca caccgcatac
aggtggcact tttcggggaa 3240 atgtgcgcgg aacccctatt tgtttatttt
tctaaataca ttcaaatatg tatccgctca 3300 tgagacaata accctgataa
atgcttcaat aatattgaaa aaggaagagt atgagtattc 3360 aacatttccg
tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 3420
acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
3480 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
gaagaacgtt 3540 ttccaatgat gagcactttt aaagttctgc tatgtgatac
actattatcc cgtattgacg 3600 ccgggcaaga gcaactcggt cgccgcatac
actattctca gaatgacttg gttgagtact 3660 caccagtcac agaaaagcat
cttacggatg gcatgacagt aagagaatta tgcagtgctg 3720 ccataaccat
gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 3780
aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
3840 aaccggagct gaatgaagcc ataccaaacg acgagagtga caccacgatg
cctgtagcaa 3900 tgccaacaac gttgcgcaaa ctattaactg gcgaactact
tactctagct tcccggcaac 3960 aattaataga ctgaatggag gcggataaag
ttgcaggacc acttctgcgc tcggcccttc 4020
cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
4080 ttgcagcact ggggccagat ggtaagcgct cccgtatcgt agttatctac
acgacgggga 4140 gtcaggcaac tatggatgaa cgaaatagac agatcgctga
gataggtgcc tcactgatta 4200 agcattggta actgtcagac caagtttact
catatatact ttagattgat ttaaaacttc 4260 atttttaatt taaaaggatc
taggtgaaga tcctttttga taatctcatg accaaaatcc 4320 cttaacgtga
gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 4380
cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
4440 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
gtaactggct 4500 tcagcagagc gcagatacca aatactgtcc ttctagtgta
gccgtagtta ggccaccact 4560 tcaagaactc tgtagcaccg cctacatacc
tcgctctgct aatcctgtta ccagtggctg 4620 ctgccagtgg cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata 4680 aggcgcagcg
gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 4740
cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
4800 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
cgcacgaggg 4860 agcttccagg gggaaacgcc tggtatcttt atagtcctgt
cgggtttcgc cacctctgac 4920 ttgagcgtcg atttttgtga tgctcgtcag
gggggcggag cctatggaaa aacgccagca 4980 acgcggcctt tttacggttc
ctgggctttt gctggccttt tgctcacatg ttctttcctg 5040 cgttatcccc
tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 5100
gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
5160 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcag 5206
<210> SEQ ID NO 10 <211> LENGTH: 5206 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: DNA sequence of pRSETB FLIP-E 10u
vector <400> SEQUENCE: 10 atctcgatcc cgcgaaatta atacgactca
ctatagggag accacaacgg tttccctcta 60 gataattttg tttaacttta
agaaggagat atacatatgc ggggttctca tcatcatcat 120 catcatggta
tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat 180
gacgataagg atccgggccg catggtgagc aagggcgagg agctgttcac cggggtggtg
240 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
gtccggcgag 300 ggcgagggcg atgccaccta cggcaagctg accctgaagt
tcatctgcac caccggcaag 360 ctgcccgtgc cctggcccac cctcgtgacc
accctgacct ggggcgtgca gtgcttcagc 420 cgctaccccg accacatgaa
gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 480 gtccaggagc
gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 540
aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
600 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
cgtctatatc 660 accgccgaca agcagaagaa cggcatcaag gccaacttca
agatccgcca caacatcgag 720 gacggcagcg tgcagctcgc cgaccactac
cagcagaaca cccccatcgg cgacggcccc 780 gtgctgctgc ccgacaacca
ctacctgagc acccagtccg ccctgagcaa agaccccaac 840 gagaagcgcg
atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 900
atggacgagc tgtacaaggg tggtaccgga ggcgccgcag gcagcacgct ggacaaaatc
960 gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt cagtgccttt
ctcttattac 1020 gacaatcagc aaaaagtggt gggttactcg caggattact
ccaacgccat tgttgaagca 1080 gtgaaaaaga aactcaacaa accggacttg
caggtaaaac tgattccgat tacctcacaa 1140 aaccgtattc cactgctgca
aaacggcact ttcgattttg aatgtggttc taccaccaac 1200 aacgtcgaac
gccaaaaaca ggcggctttc tctgacacta ttttcgtggt cggtacgcgc 1260
ctgttgacca aaaagggtgg cgatatcaaa gattttgcca acctgaaaga caaagccgta
1320 gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca aactgaatga
agagcaaaaa 1380 atgaatatgc gcatcatcag cgccaaagat cacggtgact
ctttccgcac cctggaaagc 1440 ggtcgtgccg ttgcctttat gatggatgac
cggctgctgg ccggtgaacg tgcgaaagcg 1500 aagaaaccag acaactggga
aatcgtcggc aagccgcagt ctcaggaggc ctacggttgt 1560 atgttgcgta
aagatgatcc gcagttcaaa aagctgatgg atgacaccat cgctcaggtg 1620
cagacctccg gtgaagcgga aaaatggttt gataagtggt tcaaaaatcc aattccgccg
1680 aaaaacctga acatgaattt cgaactgtca gacgaaatga aagcactgtt
caaagaaccg 1740 aatgacaagg cactgaacgg cgccggtacc ggtggaatgg
tgagcaaggg cgaggagctg 1800 ttcaccgggg tggtgcccat cctggtcgag
ctggacggcg acgtaaacgg ccacaagttc 1860 agcgtgtccg gcgagggcga
gggcgatgcc acctacggca agctgaccct gaagttcatc 1920 tgcaccaccg
gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc 1980
ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
2040 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
caactacaag 2100 acccgcgccg aggtgaagtt cgagggcgac accctggtga
accgcatcga gctgaagggc 2160 atcgacttca aggaggacgg caacatcctg
gggcacaagc tggagtacaa ctacaacagc 2220 cacaacgtct atatcatggc
cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 2280 cgccacaaca
tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2340
atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
2400 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
gaccgccgcc 2460 gggatcactc tcggcatgga cgagctgtac aagtaaaagc
ttgatccggc tgctaacaaa 2520 gcccgaaagg aagctgagtt ggctgctgcc
accgctgagc aataactagc ataacccctt 2580 ggggcctcta aacgggtctt
gaggggtttt ttgctgaaag gaggaactat atccggatct 2640 ggcgtaatag
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 2700
gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
2760 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
ttcccttcct 2820 ttctcgccac gttcgccggc tttccccgtc aagctctaaa
tcgggggctc cctttagggt 2880 tccgatttag agctttacgg cacctcgacc
gcaaaaaact tgatttgggt gatggttcac 2940 gtagtgggcc atcgccctga
tagacggttt ttcgcccttt gacgttggag tccacgttct 3000 ttaatagtgg
actcttgttc caaactggaa caacactcaa ccctatcgcg gtctattctt 3060
ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3120 aaatatttaa cgcgaatttt aacaaaatat taacgtttac aatttcgcct
gatgcggtat 3180 tttctcctta cgcatctgtg cggtatttca caccgcatac
aggtggcact tttcggggaa 3240 atgtgcgcgg aacccctatt tgtttatttt
tctaaataca ttcaaatatg tatccgctca 3300 tgagacaata accctgataa
atgcttcaat aatattgaaa aaggaagagt atgagtattc 3360 aacatttccg
tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 3420
acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
3480 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
gaagaacgtt 3540 ttccaatgat gagcactttt aaagttctgc tatgtgatac
actattatcc cgtattgacg 3600 ccgggcaaga gcaactcggt cgccgcatac
actattctca gaatgacttg gttgagtact 3660 caccagtcac agaaaagcat
cttacggatg gcatgacagt aagagaatta tgcagtgctg 3720 ccataaccat
gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 3780
aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
3840 aaccggagct gaatgaagcc ataccaaacg acgagagtga caccacgatg
cctgtagcaa 3900 tgccaacaac gttgcgcaaa ctattaactg gcgaactact
tactctagct tcccggcaac 3960 aattaataga ctgaatggag gcggataaag
ttgcaggacc acttctgcgc tcggcccttc 4020 cggctggctg gtttattgct
gataaatctg gagccggtga gcgtgggtct cgcggtatca 4080 ttgcagcact
ggggccagat ggtaagcgct cccgtatcgt agttatctac acgacgggga 4140
gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
4200 agcattggta actgtcagac caagtttact catatatact ttagattgat
ttaaaacttc 4260 atttttaatt taaaaggatc taggtgaaga tcctttttga
taatctcatg accaaaatcc 4320 cttaacgtga gttttcgttc cactgagcgt
cagaccccgt agaaaagatc aaaggatctt 4380 cttgagatcc tttttttctg
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4440 cagcggtggt
ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4500
tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
4560 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
ccagtggctg 4620 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
aagacgatag ttaccggata 4680 aggcgcagcg gtcgggctga acggggggtt
cgtgcacaca gcccagcttg gagcgaacga 4740 cctacaccga actgagatac
ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4800 ggagaaaggc
ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4860
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
4920 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
aacgccagca 4980 acgcggcctt tttacggttc ctgggctttt gctggccttt
tgctcacatg ttctttcctg 5040 cgttatcccc tgattctgtg gataaccgta
ttaccgcctt tgagtgagct gataccgctc 5100 gccgcagccg aacgaccgag
cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 5160 tacgcaaacc
gcctctcccc gcgcgttggc cgattcatta atgcag 5206 <210> SEQ ID NO
11 <211> LENGTH: 7603 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: DNA sequence of pDisplay FLIP-E 600n <400>
SEQUENCE: 11 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg
gggtcattag 60 ttcatagccc atatatggag ttccgcgtta cataacttac
ggtaaatggc ccgcctggct 120 gaccgcccaa cgacccccgc ccattgacgt
caataatgac gtatgttccc atagtaacgc 180 caatagggac tttccattga
cgtcaatggg tggactattt acggtaaact gcccacttgg 240 cagtacatca
agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 300
ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
360 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
atcaatgggc 420 gtggatagcg gtttgactca cggggatttc caagtctcca
ccccattgac gtcaatggga 480 gtttgttttg gcaccaaaat caacgggact
ttccaaaatg tcgtaacaac tccgccccat 540
tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
600 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
tatagggaga 660 cccaagcttg gtaccgagct cggatccact agtaacggcc
gccagtgtgc tggaattcgg 720 cttggggata tccaccatgg agacagacac
actcctgcta tgggtactgc tgctctgggt 780 tccaggttcc actggtgact
atccatatga tgttccagat tatgctgggg cccagccggc 840 cagatctccc
gggatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 900
ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
960 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
agctgcccgt 1020 gccctggccc accctcgtga ccaccctgac ctggggcgtg
cagtgcttca gccgctaccc 1080 cgaccacatg aagcagcacg acttcttcaa
gtccgccatg cccgaaggct acgtccagga 1140 gcgcaccatc ttcttcaagg
acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 1200 gggcgacacc
ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 1260
catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
1320 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
aggacggcag 1380 cgtgcagctc gccgaccact accagcagaa cacccccatc
ggcgacggcc ccgtgctgct 1440 gcccgacaac cactacctga gcacccagtc
cgccctgagc aaagacccca acgagaagcg 1500 cgatcacatg gtcctgctgg
agttcgtgac cgccgccggg atcactctcg gcatggacga 1560 gctgtacaag
ggtggtaccg gaggcgccgc aggcagcacg ctggacaaaa tcgccaaaaa 1620
cggtgtgatt gtcgtcggtc accgtgaatc ttcagtgcct ttctcttatt acgacaatca
1680 gcaaaaagtg gtgggttact cgcaggatta ctccaacgcc attgttgaag
cagtgaaaaa 1740 gaaactcaac aaaccggact tgcaggtaaa actgattccg
attacctcac aaaaccgtat 1800 tccactgctg caaaacggca ctttcgattt
tgaatgtggt tctaccacca acaacgtcga 1860 acgccaaaaa caggcggctt
tctctgacac tattttcgtg gtcggtacgc gcctgttgac 1920 caaaaagggt
ggcgatatca aagattttgc caacctgaaa gacaaagccg tagtcgtcac 1980
ttccggcact acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat
2040 gcgcatcatc agcgccaaag atcacggtga ctctttccgc accctggaaa
gcggtcgtgc 2100 cgttgccttt atgatggatg acgctctgct ggccggtgaa
cgtgcgaaag cgaagaaacc 2160 agacaactgg gaaatcgtcg gcaagccgca
gtctcaggag gcctacggtt gtatgttgcg 2220 taaagatgat ccgcagttca
aaaagctgat ggatgacacc atcgctcagg tgcagacctc 2280 cggtgaagcg
gaaaaatggt ttgataagtg gttcaaaaat ccaattccgc cgaaaaacct 2340
gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa
2400 ggcactgaac ggcgccggta ccggtggaat ggtgagcaag ggcgaggagc
tgttcaccgg 2460 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
ggccacaagt tcagcgtgtc 2520 cggcgagggc gagggcgatg ccacctacgg
caagctgacc ctgaagttca tctgcaccac 2580 cggcaagctg cccgtgccct
ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg 2640 cttcgcccgc
taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 2700
aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
2760 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
gcatcgactt 2820 caaggaggac ggcaacatcc tggggcacaa gctggagtac
aactacaaca gccacaacgt 2880 ctatatcatg gccgacaagc agaagaacgg
catcaaggtg aacttcaaga tccgccacaa 2940 catcgaggac ggcagcgtgc
agctcgccga ccactaccag cagaacaccc ccatcggcga 3000 cggccccgtg
ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga 3060
ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
3120 tctcggcatg gacgagctgt acaaggtcga cgaacaaaaa ctcatctcag
aagaggatct 3180 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg
ccacactcct tgccctttaa 3240 ggtggtggtg atctcagcca tcctggccct
ggtggtgctc accatcatct cccttatcat 3300 cctcatcatg ctttggcaga
agaagccacg ttaggcggcc gctcgagatc agcctcgact 3360 gtgccttcta
gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 3420
gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
3480 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
ggaggattgg 3540 gaagacaata gcaggcatgc tggggatgcg gtgggctcta
tggcttctga ggcggaaaga 3600 accagtggcg gtaatacggt tatccacaga
atcaggggat aacgcaggaa agaacatgtg 3660 agcaaaaggc cagcaaaagg
ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3720 taggctccgc
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3780
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
3840 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
gaagcgtggc 3900 gctttctcat agctcacgct gtaggtatct cagttcggtg
taggtcgttc gctccaagct 3960 gggctgtgtg cacgaacccc ccgttcagcc
cgaccgctgc gccttatccg gtaactatcg 4020 tcttgagtcc aacccggtaa
gacacgactt atcgccactg gcagcagcca ctggtaacag 4080 gattagcaga
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4140
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
4200 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
gtggtttttt 4260 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
caagaagatc ctttgatctt 4320 ttctacgggg tctgacgctc agtggaacga
aaactcacgt taagggattt tggtcatgag 4380 attatcaaaa aggatcttca
cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4440 ctaaagtata
tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc 4500
gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
4560 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct
gggaccgaac 4620 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
ttctgtcttt ttattgccgt 4680 catagcgcgg gttccttccg gtattgtctc
cttccgtgtt tcagttagcc tccccctagg 4740 gtgggcgaag aactccagca
tgagatcccc gcgctggagg atcatccagc cggcgtcccg 4800 gaaaacgatt
ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga 4860
tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
4920 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
accgtaaagc 4980 acgaggaagc ggtcagccca ttcgccgcca agctcttcag
caatatcacg ggtagccaac 5040 gctatgtcct gatagcggtc cgccacaccc
agccggccac agtcgatgaa tccagaaaag 5100 cggccatttt ccaccatgat
attcggcaag caggcatcgc catgggtcac gacgagatcc 5160 tcgccgtcgg
gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga 5220
tgctcttgat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
5280 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
cagccgccgc 5340 attgcatcag ccatgatgga tactttctcg gcaggagcaa
ggtgagatga caggagatcc 5400 tgccccggca cttcgcccaa tagcagccag
tcccttcccg cttcagtgac aacgtcgagc 5460 acagctgcgc aaggaacgcc
cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc 5520 agttcattca
gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 5580
gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
5640 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
ttcaatcatg 5700 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa
aagcctaggc ctccaaaaaa 5760 gcctcctcac tacttctgga atagctcaga
ggccgaggag gcggcctcgg cctctgcata 5820 aataaaaaaa attagtcagc
catggggcgg agaatgggcg gaactgggcg gagttagggg 5880 cgggatgggc
ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg 5940
catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
6000 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
accctaactg 6060 acacacattc cacagctggt tctttccgcc tcaggactct
tcctttttca ataaatcaat 6120 ctaaagtata tatgagtaaa cttggtctga
cagttaccaa tgcttaatca gtgaggcacc 6180 tatctcagcg atctgtctat
ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6240 aactacgata
cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 6300
acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
6360 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
gggaagctag 6420 agtaagtagt tcgccagtta atagtttgcg caacgttgtt
gccattgcta caggcatcgt 6480 ggtgtcacgc tcgtcgtttg gtatggcttc
attcagctcc ggttcccaac gatcaaggcg 6540 agttacatga tcccccatgt
tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6600 tgtcagaagt
aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6660
tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
6720 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
tacgggataa 6780 taccgcgcca catagcagaa ctttaaaagt gctcatcatt
ggaaaacgtt cttcggggcg 6840 aaaactctca aggatcttac cgctgttgag
atccagttcg atgtaaccca ctcgtgcacc 6900 caactgatct tcagcatctt
ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6960 gcaaaatgcc
gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7020
cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
7080 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
gaaaagtgcc 7140 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
gtggtggtta cgcgcagcgt 7200 gaccgctaca cttgccagcg ccctagcgcc
cgctcctttc gctttcttcc cttcctttct 7260 cgccacgttc gccggctttc
cccgtcaagc tctaaatcgg gggctccctt tagggttccg 7320 atttagtgct
ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 7380
tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
7440 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
attcttttga 7500 tttataaggg attttgccga tttcggccta ttggttaaaa
aatgagctga tttaacaaaa 7560 atttaacgcg aattttaaca aaatattaac
gcttacaatt tac 7603 <210> SEQ ID NO 12 <211> LENGTH:
7603 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: DNA
sequence of pDisplay FLIP-E 10u <400> SEQUENCE: 12 gcgcgcgttg
acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 60
ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
120
gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
180 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact
gcccacttgg 240 cagtacatca agtgtatcat atgccaagta cgccccctat
tgacgtcaat gacggtaaat 300 ggcccgcctg gcattatgcc cagtacatga
ccttatggga ctttcctact tggcagtaca 360 tctacgtatt agtcatcgct
attaccatgg tgatgcggtt ttggcagtac atcaatgggc 420 gtggatagcg
gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 480
gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
540 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
gctctctggc 600 taactagaga acccactgct tactggctta tcgaaattaa
tacgactcac tatagggaga 660 cccaagcttg gtaccgagct cggatccact
agtaacggcc gccagtgtgc tggaattcgg 720 cttggggata tccaccatgg
agacagacac actcctgcta tgggtactgc tgctctgggt 780 tccaggttcc
actggtgact atccatatga tgttccagat tatgctgggg cccagccggc 840
cagatctccc gggatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
900 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
agggcgaggg 960 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
accaccggca agctgcccgt 1020 gccctggccc accctcgtga ccaccctgac
ctggggcgtg cagtgcttca gccgctaccc 1080 cgaccacatg aagcagcacg
acttcttcaa gtccgccatg cccgaaggct acgtccagga 1140 gcgcaccatc
ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 1200
gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
1260 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata
tcaccgccga 1320 caagcagaag aacggcatca aggccaactt caagatccgc
cacaacatcg aggacggcag 1380 cgtgcagctc gccgaccact accagcagaa
cacccccatc ggcgacggcc ccgtgctgct 1440 gcccgacaac cactacctga
gcacccagtc cgccctgagc aaagacccca acgagaagcg 1500 cgatcacatg
gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 1560
gctgtacaag ggtggtaccg gaggcgccgc aggcagcacg ctggacaaaa tcgccaaaaa
1620 cggtgtgatt gtcgtcggtc accgtgaatc ttcagtgcct ttctcttatt
acgacaatca 1680 gcaaaaagtg gtgggttact cgcaggatta ctccaacgcc
attgttgaag cagtgaaaaa 1740 gaaactcaac aaaccggact tgcaggtaaa
actgattccg attacctcac aaaaccgtat 1800 tccactgctg caaaacggca
ctttcgattt tgaatgtggt tctaccacca acaacgtcga 1860 acgccaaaaa
caggcggctt tctctgacac tattttcgtg gtcggtacgc gcctgttgac 1920
caaaaagggt ggcgatatca aagattttgc caacctgaaa gacaaagccg tagtcgtcac
1980 ttccggcact acctctgaag ttttgctcaa caaactgaat gaagagcaaa
aaatgaatat 2040 gcgcatcatc agcgccaaag atcacggtga ctctttccgc
accctggaaa gcggtcgtgc 2100 cgttgccttt atgatggatg accggctgct
ggccggtgaa cgtgcgaaag cgaagaaacc 2160 agacaactgg gaaatcgtcg
gcaagccgca gtctcaggag gcctacggtt gtatgttgcg 2220 taaagatgat
ccgcagttca aaaagctgat ggatgacacc atcgctcagg tgcagacctc 2280
cggtgaagcg gaaaaatggt ttgataagtg gttcaaaaat ccaattccgc cgaaaaacct
2340 gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac
cgaatgacaa 2400 ggcactgaac ggcgccggta ccggtggaat ggtgagcaag
ggcgaggagc tgttcaccgg 2460 ggtggtgccc atcctggtcg agctggacgg
cgacgtaaac ggccacaagt tcagcgtgtc 2520 cggcgagggc gagggcgatg
ccacctacgg caagctgacc ctgaagttca tctgcaccac 2580 cggcaagctg
cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg 2640
cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
2700 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
agacccgcgc 2760 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
gagctgaagg gcatcgactt 2820 caaggaggac ggcaacatcc tggggcacaa
gctggagtac aactacaaca gccacaacgt 2880 ctatatcatg gccgacaagc
agaagaacgg catcaaggtg aacttcaaga tccgccacaa 2940 catcgaggac
ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 3000
cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
3060 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
ccgggatcac 3120 tctcggcatg gacgagctgt acaaggtcga cgaacaaaaa
ctcatctcag aagaggatct 3180 gaatgctgtg ggccaggaca cgcaggaggt
catcgtggtg ccacactcct tgccctttaa 3240 ggtggtggtg atctcagcca
tcctggccct ggtggtgctc accatcatct cccttatcat 3300 cctcatcatg
ctttggcaga agaagccacg ttaggcggcc gctcgagatc agcctcgact 3360
gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
3420 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
gcattgtctg 3480 agtaggtgtc attctattct ggggggtggg gtggggcagg
acagcaaggg ggaggattgg 3540 gaagacaata gcaggcatgc tggggatgcg
gtgggctcta tggcttctga ggcggaaaga 3600 accagtggcg gtaatacggt
tatccacaga atcaggggat aacgcaggaa agaacatgtg 3660 agcaaaaggc
cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3720
taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
3780 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
tgcgctctcc 3840 tgttccgacc ctgccgctta ccggatacct gtccgccttt
ctcccttcgg gaagcgtggc 3900 gctttctcat agctcacgct gtaggtatct
cagttcggtg taggtcgttc gctccaagct 3960 gggctgtgtg cacgaacccc
ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4020 tcttgagtcc
aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4080
gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
4140 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
ttaccttcgg 4200 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
gctggtagcg gtggtttttt 4260 tgtttgcaag cagcagatta cgcgcagaaa
aaaaggatct caagaagatc ctttgatctt 4320 ttctacgggg tctgacgctc
agtggaacga aaactcacgt taagggattt tggtcatgag 4380 attatcaaaa
aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4440
ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
4500 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag
aaacgcgggc 4560 gtattggccc caatggggtc tcggtggggt atcgacagag
tgccagccct gggaccgaac 4620 cccgcgttta tgaacaaacg acccaacacc
gtgcgtttta ttctgtcttt ttattgccgt 4680 catagcgcgg gttccttccg
gtattgtctc cttccgtgtt tcagttagcc tccccctagg 4740 gtgggcgaag
aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg 4800
gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
4860 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
tcagaagaac 4920 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
gagcggcgat accgtaaagc 4980 acgaggaagc ggtcagccca ttcgccgcca
agctcttcag caatatcacg ggtagccaac 5040 gctatgtcct gatagcggtc
cgccacaccc agccggccac agtcgatgaa tccagaaaag 5100 cggccatttt
ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc 5160
tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
5220 tgctcttgat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
tcgctcgatg 5280 cgatgtttcg cttggtggtc gaatgggcag gtagccggat
caagcgtatg cagccgccgc 5340 attgcatcag ccatgatgga tactttctcg
gcaggagcaa ggtgagatga caggagatcc 5400 tgccccggca cttcgcccaa
tagcagccag tcccttcccg cttcagtgac aacgtcgagc 5460 acagctgcgc
aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc 5520
agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
5580 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
gtcatagccg 5640 aatagcctct ccacccaagc ggccggagaa cctgcgtgca
atccatcttg ttcaatcatg 5700 cgaaacgatc ctcatcctgt ctcttgatcg
atctttgcaa aagcctaggc ctccaaaaaa 5760 gcctcctcac tacttctgga
atagctcaga ggccgaggag gcggcctcgg cctctgcata 5820 aataaaaaaa
attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg 5880
cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
5940 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
actaattgag 6000 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
gactttccac accctaactg 6060 acacacattc cacagctggt tctttccgcc
tcaggactct tcctttttca ataaatcaat 6120 ctaaagtata tatgagtaaa
cttggtctga cagttaccaa tgcttaatca gtgaggcacc 6180 tatctcagcg
atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6240
aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
6300 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
ccgagcgcag 6360 aagtggtcct gcaactttat ccgcctccat ccagtctatt
aattgttgcc gggaagctag 6420 agtaagtagt tcgccagtta atagtttgcg
caacgttgtt gccattgcta caggcatcgt 6480 ggtgtcacgc tcgtcgtttg
gtatggcttc attcagctcc ggttcccaac gatcaaggcg 6540 agttacatga
tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6600
tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
6660 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
caaccaagtc 6720 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
ccggcgtcaa tacgggataa 6780 taccgcgcca catagcagaa ctttaaaagt
gctcatcatt ggaaaacgtt cttcggggcg 6840 aaaactctca aggatcttac
cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6900 caactgatct
tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6960
gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
7020 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
gatacatatt 7080 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
acatttcccc gaaaagtgcc 7140 acctgacgcg ccctgtagcg gcgcattaag
cgcggcgggt gtggtggtta cgcgcagcgt 7200 gaccgctaca cttgccagcg
ccctagcgcc cgctcctttc gctttcttcc cttcctttct 7260 cgccacgttc
gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 7320
atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
7380 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
cgttctttaa 7440 tagtggactc ttgttccaaa ctggaacaac actcaaccct
atctcggtct attcttttga 7500 tttataaggg attttgccga tttcggccta
ttggttaaaa aatgagctga tttaacaaaa 7560 atttaacgcg aattttaaca
aaatattaac gcttacaatt tac 7603
<210> SEQ ID NO 13 <211> LENGTH: 799 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Amino acid sequence of FLIP-E 600n
(expressed as 6xHis fusion in pRSET FLIP-E 600n) <400>
SEQUENCE: 13 Met Arg Gly Ser His His His His His His Gly Met Ala
Ser Met Thr 1 5 10 15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp
Asp Asp Asp Lys Asp 20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu
Glu Leu Phe Thr Gly Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp
Gly Asp Val Asn Gly His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly
Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val
Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105
110 His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr
115 120 125 Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr
Lys Thr 130 135 140 Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val
Asn Arg Ile Glu 145 150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp
Gly Asn Ile Leu Gly His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser
His Asn Val Tyr Ile Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile
Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly
Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230
235 240 Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val
Leu 245 250 255 Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met
Asp Glu Leu 260 265 270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Gly Ser
Thr Leu Asp Lys Ile 275 280 285 Ala Lys Asn Gly Val Ile Val Val Gly
His Arg Glu Ser Ser Val Pro 290 295 300 Phe Ser Tyr Tyr Asp Asn Gln
Gln Lys Val Val Gly Tyr Ser Gln Asp 305 310 315 320 Tyr Ser Asn Ala
Ile Val Glu Ala Val Lys Lys Lys Leu Asn Lys Pro 325 330 335 Asp Leu
Gln Val Lys Leu Ile Pro Ile Thr Ser Gln Asn Arg Ile Pro 340 345 350
Leu Leu Gln Asn Gly Thr Phe Asp Phe Glu Cys Gly Ser Thr Thr Asn 355
360 365 Asn Val Glu Arg Gln Lys Gln Ala Ala Phe Ser Asp Thr Ile Phe
Val 370 375 380 Val Gly Thr Arg Leu Leu Thr Lys Lys Gly Gly Asp Ile
Lys Asp Phe 385 390 395 400 Ala Asn Leu Lys Asp Lys Ala Val Val Val
Thr Ser Gly Thr Thr Ser 405 410 415 Glu Val Leu Leu Asn Lys Leu Asn
Glu Glu Gln Lys Met Asn Met Arg 420 425 430 Ile Ile Ser Ala Lys Asp
His Gly Asp Ser Phe Arg Thr Leu Glu Ser 435 440 445 Gly Arg Ala Val
Ala Phe Met Met Asp Asp Ala Leu Leu Ala Gly Glu 450 455 460 Arg Ala
Lys Ala Lys Lys Pro Asp Asn Trp Glu Ile Val Gly Lys Pro 465 470 475
480 Gln Ser Gln Glu Ala Tyr Gly Cys Met Leu Arg Lys Asp Asp Pro Gln
485 490 495 Phe Lys Lys Leu Met Asp Asp Thr Ile Ala Gln Val Gln Thr
Ser Gly 500 505 510 Glu Ala Glu Lys Trp Phe Asp Lys Trp Phe Lys Asn
Pro Ile Pro Pro 515 520 525 Lys Asn Leu Asn Met Asn Phe Glu Leu Ser
Asp Glu Met Lys Ala Leu 530 535 540 Phe Lys Glu Pro Asn Asp Lys Ala
Leu Asn Gly Ala Gly Thr Gly Gly 545 550 555 560 Met Val Ser Lys Gly
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 565 570 575 Val Glu Leu
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 580 585 590 Glu
Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 595 600
605 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr
610 615 620 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His
Met Lys 625 630 635 640 Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu
Gly Tyr Val Gln Glu 645 650 655 Arg Thr Ile Phe Phe Lys Asp Asp Gly
Asn Tyr Lys Thr Arg Ala Glu 660 665 670 Val Lys Phe Glu Gly Asp Thr
Leu Val Asn Arg Ile Glu Leu Lys Gly 675 680 685 Ile Asp Phe Lys Glu
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 690 695 700 Asn Tyr Asn
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 705 710 715 720
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 725
730 735 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly 740 745 750 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln
Ser Ala Leu 755 760 765 Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met
Val Leu Leu Glu Phe 770 775 780 Val Thr Ala Ala Gly Ile Thr Leu Gly
Met Asp Glu Leu Tyr Lys 785 790 795 <210> SEQ ID NO 14
<211> LENGTH: 764 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Amino acid sequence of FLIP-E 10u (expressed as 6xHis
fusion in pRSET FLIP-E 10u) <400> SEQUENCE: 14 Met Val Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 1 5 10 15 Val
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 20 25
30 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile
35 40 45 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val
Thr Thr 50 55 60 Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro
Asp His Met Lys 65 70 75 80 Gln His Asp Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr Val Gln Glu 85 90 95 Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys Thr Arg Ala Glu 100 105 110 Val Lys Phe Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 115 120 125 Ile Asp Phe Lys
Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 130 135 140 Asn Tyr
Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 145 150 155
160 Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser
165 170 175 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly
Asp Gly 180 185 190 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr
Gln Ser Ala Leu 195 200 205 Ser Lys Asp Pro Asn Glu Lys Arg Asp His
Met Val Leu Leu Glu Phe 210 215 220 Val Thr Ala Ala Gly Ile Thr Leu
Gly Met Asp Glu Leu Tyr Lys Gly 225 230 235 240 Gly Thr Gly Gly Ala
Ala Gly Ser Thr Leu Asp Lys Ile Ala Lys Asn 245 250 255 Gly Val Ile
Val Val Gly His Arg Glu Ser Ser Val Pro Phe Ser Tyr 260 265 270 Tyr
Asp Asn Gln Gln Lys Val Val Gly Tyr Ser Gln Asp Tyr Ser Asn 275 280
285 Ala Ile Val Glu Ala Val Lys Lys Lys Leu Asn Lys Pro Asp Leu Gln
290 295 300 Val Lys Leu Ile Pro Ile Thr Ser Gln Asn Arg Ile Pro Leu
Leu Gln 305 310 315 320 Asn Gly Thr Phe Asp Phe Glu Cys Gly Ser Thr
Thr Asn Asn Val Glu 325 330 335 Arg Gln Lys Gln Ala Ala Phe Ser Asp
Thr Ile Phe Val Val Gly Thr 340 345 350 Arg Leu Leu Thr Lys Lys Gly
Gly Asp Ile Lys Asp Phe Ala Asn Leu 355 360 365 Lys Asp Lys Ala Val
Val Val Thr Ser Gly Thr Thr Ser Glu Val Leu 370 375 380 Leu Asn Lys
Leu Asn Glu Glu Gln Lys Met Asn Met Arg Ile Ile Ser 385 390 395 400
Ala Lys Asp His Gly Asp Ser Phe Arg Thr Leu Glu Ser Gly Arg Ala 405
410 415
Val Ala Phe Met Met Asp Asp Arg Leu Leu Ala Gly Glu Arg Ala Lys 420
425 430 Ala Lys Lys Pro Asp Asn Trp Glu Ile Val Gly Lys Pro Gln Ser
Gln 435 440 445 Glu Ala Tyr Gly Cys Met Leu Arg Lys Asp Asp Pro Gln
Phe Lys Lys 450 455 460 Leu Met Asp Asp Thr Ile Ala Gln Val Gln Thr
Ser Gly Glu Ala Glu 465 470 475 480 Lys Trp Phe Asp Lys Trp Phe Lys
Asn Pro Ile Pro Pro Lys Asn Leu 485 490 495 Asn Met Asn Phe Glu Leu
Ser Asp Glu Met Lys Ala Leu Phe Lys Glu 500 505 510 Pro Asn Asp Lys
Ala Leu Asn Gly Ala Gly Thr Gly Gly Met Val Ser 515 520 525 Lys Gly
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 530 535 540
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 545
550 555 560 Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys
Thr Thr 565 570 575 Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr
Thr Phe Gly Tyr 580 585 590 Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp
His Met Lys Gln His Asp 595 600 605 Phe Phe Lys Ser Ala Met Pro Glu
Gly Tyr Val Gln Glu Arg Thr Ile 610 615 620 Phe Phe Lys Asp Asp Gly
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 625 630 635 640 Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 645 650 655 Lys
Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 660 665
670 Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys
675 680 685 Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val
Gln Leu 690 695 700 Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly Pro Val Leu 705 710 715 720 Leu Pro Asp Asn His Tyr Leu Ser Tyr
Gln Ser Ala Leu Ser Lys Asp 725 730 735 Pro Asn Glu Lys Arg Asp His
Met Val Leu Leu Glu Phe Val Thr Ala 740 745 750 Ala Gly Ile Thr Leu
Gly Met Asp Glu Leu Tyr Lys 755 760 <210> SEQ ID NO 15
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-mglBF16A-Y12S-CFP-D13R-YFP Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 15 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc gct gat act cgc att ggt gta aca atc tat aag tcg gct 144 Pro
Gly Arg Ala Asp Thr Arg Ile Gly Val Thr Ile Tyr Lys Ser Ala 35 40
45 ggt atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc
192 Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile
50 55 60 ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
gtg tcc 240 Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
Val Ser 65 70 75 80 ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg aag ttc 288 Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu Lys Phe 85 90 95 atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc gtg acc 336 Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu Val Thr 100 105 110 acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac cac atg 384 Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp His Met 115 120 125 aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac gtc cag 432 Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 130 135 140 gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc cgc gcc 480 Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 145 150 155 160 gag
gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag 528 Glu
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 165 170
175 ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag
576 Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu
180 185 190 tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
cag aag 624 Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
Gln Lys 195 200 205 aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag gac ggc 672 Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu Asp Gly 210 215 220 agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc ggc gac 720 Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile Gly Asp 225 230 235 240 ggc ccc gtg ctg ctg ccc gac
aac cac tac ctg agc acc cag tcc gcc 768 Gly Pro Val Leu Leu Pro Asp
Asn His Tyr Leu Ser Thr Gln Ser Ala 245 250 255 ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg ctg gag 816 Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 260 265 270 ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg tac ggt 864 Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Gly 275 280 285 agc
cga gat aac gcg atg tct gta gtg cgc aag gct att gag caa gat 912 Ser
Arg Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp 290 295
300 gcg aaa gcc gcg cca gat gtt cag ctg ctg atg aat gat tct cag aat
960 Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn
305 310 315 320 gac cag tcc aag cag aac gat cag atc gac gta ttg ctg
gcg aaa ggg 1008 Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp Val Leu
Leu Ala Lys Gly 325 330 335 gtg aag gca ctg gca atc aac ctg gtt gac
ccg gca gct gcg ggt acg 1056 Val Lys Ala Leu Ala Ile Asn Leu Val
Asp Pro Ala Ala Ala Gly Thr 340 345 350 gtg att gag aaa gcg cgt ggg
caa aac gtg ccg gtg gtt ttc ttc aac 1104 Val Ile Glu Lys Ala Arg
Gly Gln Asn Val Pro Val Val Phe Phe Asn 355 360 365 aaa gaa ccg tct
cgt aag gcg ctg gat agc tac gac aaa gcc tac tac 1152 Lys Glu Pro
Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala Tyr Tyr 370 375 380 gtt
ggc act gac tcc aaa gag tcc ggc att att caa ggc gat ttg att 1200
Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile 385
390 395 400 gct aaa cac tgg gcg gcg aat cag ggt tgg gat ctg aac aaa
gac ggt 1248 Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn
Lys Asp Gly 405 410 415 cag att cag ttc gta ctg ctg aaa ggt gaa ccg
ggc cat ccg gat gca 1296 Gln Ile Gln Phe Val Leu Leu Lys Gly Glu
Pro Gly His Pro Asp Ala 420 425 430 gaa gca cgt acc act tac gtg att
aaa gaa ttg aac gat aaa ggc atc 1344 Glu Ala Arg Thr Thr Tyr Val
Ile Lys Glu Leu Asn Asp Lys Gly Ile 435 440 445 aaa act gaa cag tta
cag tta gat acc gca atg tgg gac acc gct cag 1392 Lys Thr Glu Gln
Leu Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln 450 455 460 gcg aaa
gat aag atg gac gcc tgg ctg tct ggc ccg aac gcc aac aaa 1440 Ala
Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys 465 470
475 480 atc gaa gtg gtt atc gcc aac aac gat gcg atg gca atg ggc gcg
gtt 1488 Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly
Ala Val 485 490 495 gaa gcg ctg aaa gca cac aac aag tcc agc att ccg
gtg ttt ggc gtc 1536 Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile
Pro Val Phe Gly Val 500 505 510 gat gcg ctg cca gaa gcg ctg gcg ctg
gtg aaa tcc ggt gca ctg gcg 1584 Asp Ala Leu Pro Glu Ala Leu Ala
Leu Val Lys Ser Gly Ala Leu Ala 515 520 525 ggc acc gta ctg aac gat
gct aac aac cag gcg aaa gcg acc ttt gat 1632 Gly Thr Val Leu Asn
Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe Asp 530 535 540 ctg gcg aaa
aac ctg gcc gat ggt aaa ggt gcg gct gat ggc acc aac 1680 Leu Ala
Lys Asn Leu Ala Asp Gly Lys Gly Ala Ala Asp Gly Thr Asn 545 550 555
560 tgg aaa atc gac aac aaa gtg gtc cgc gta cct tat gtt ggc gta gat
1728 Trp Lys Ile Asp Asn Lys Val Val Arg Val Pro Tyr Val Gly Val
Asp 565 570 575 aaa gac aac ctg gct gaa ttc agc aag aaa ggc gcc ggt
acc ggt gga 1776 Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys Gly Ala
Gly Thr Gly Gly 580 585 590 atg gtg agc aag ggc gag gag ctg ttc acc
ggg gtg gtg ccc atc ctg 1824 Met Val Ser Lys Gly Glu Glu Leu Phe
Thr Gly Val Val Pro Ile Leu 595 600 605 gtc gag ctg gac ggc gac gta
aac ggc cac aag ttc agc gtg tcc ggc 1872 Val Glu Leu Asp Gly Asp
Val Asn Gly His Lys Phe Ser Val Ser Gly 610 615 620 gag ggc gag ggc
gat gcc acc tac ggc aag ctg acc ctg aag ttc atc 1920 Glu Gly Glu
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 625 630 635 640
tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc
1968 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr
Thr 645 650 655 ttc ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac
cac atg aag 2016 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro
Asp His Met Lys 660 665 670 cag cac gac ttc ttc aag tcc gcc atg ccc
gaa ggc tac gtc cag gag 2064 Gln His Asp Phe Phe Lys Ser Ala Met
Pro Glu Gly Tyr Val Gln Glu 675 680 685
cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag
2112 Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala
Glu 690 695 700 gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag
ctg aag ggc 2160 Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile
Glu Leu Lys Gly 705 710 715 720 atc gac ttc aag gag gac ggc aac atc
ctg ggg cac aag ctg gag tac 2208 Ile Asp Phe Lys Glu Asp Gly Asn
Ile Leu Gly His Lys Leu Glu Tyr 725 730 735 aac tac aac agc cac aac
gtc tat atc atg gcc gac aag cag aag aac 2256 Asn Tyr Asn Ser His
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 740 745 750 ggc atc aag
gtg aac ttc aag atc cgc cac aac atc gag gac ggc agc 2304 Gly Ile
Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 755 760 765
gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc
2352 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly 770 775 780 ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag
tcc gcc ctg 2400 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr
Gln Ser Ala Leu 785 790 795 800 agc aaa gac ccc aac gag aag cgc gat
cac atg gtc ctg ctg gag ttc 2448 Ser Lys Asp Pro Asn Glu Lys Arg
Asp His Met Val Leu Leu Glu Phe 805 810 815 gtg acc gcc gcc ggg atc
act ctc ggc atg gac gag ctg tac aag taa 2496 Val Thr Ala Ala Gly
Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 820 825 830 <210> SEQ
ID NO 16 <211> LENGTH: 831 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-mglBF16A-Y12S-CFP-D13R-YFP Vector
<400> SEQUENCE: 16 Met Arg Gly Ser His His His His His His
Gly Met Ala Ser Met Thr 1 5 10 15 Gly Gly Gln Gln Met Gly Arg Asp
Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 Pro Gly Arg Ala Asp Thr
Arg Ile Gly Val Thr Ile Tyr Lys Ser Ala 35 40 45 Gly Met Val Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 50 55 60 Leu Val
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 65 70 75 80
Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 85
90 95 Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val
Thr 100 105 110 Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro
Asp His Met 115 120 125 Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr Val Gln 130 135 140 Glu Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys Thr Arg Ala 145 150 155 160 Glu Val Lys Phe Glu Gly
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 165 170 175 Gly Ile Asp Phe
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 180 185 190 Tyr Asn
Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys 195 200 205
Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 210
215 220 Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly
Asp 225 230 235 240 Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser
Thr Gln Ser Ala 245 250 255 Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp
His Met Val Leu Leu Glu 260 265 270 Phe Val Thr Ala Ala Gly Ile Thr
Leu Gly Met Asp Glu Leu Tyr Gly 275 280 285 Ser Arg Asp Asn Ala Met
Ser Val Val Arg Lys Ala Ile Glu Gln Asp 290 295 300 Ala Lys Ala Ala
Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn 305 310 315 320 Asp
Gln Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly 325 330
335 Val Lys Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala Gly Thr
340 345 350 Val Ile Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val Phe
Phe Asn 355 360 365 Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp
Lys Ala Tyr Tyr 370 375 380 Val Gly Thr Asp Ser Lys Glu Ser Gly Ile
Ile Gln Gly Asp Leu Ile 385 390 395 400 Ala Lys His Trp Ala Ala Asn
Gln Gly Trp Asp Leu Asn Lys Asp Gly 405 410 415 Gln Ile Gln Phe Val
Leu Leu Lys Gly Glu Pro Gly His Pro Asp Ala 420 425 430 Glu Ala Arg
Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile 435 440 445 Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln 450 455
460 Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys
465 470 475 480 Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met Ala Met
Gly Ala Val 485 490 495 Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile
Pro Val Phe Gly Val 500 505 510 Asp Ala Leu Pro Glu Ala Leu Ala Leu
Val Lys Ser Gly Ala Leu Ala 515 520 525 Gly Thr Val Leu Asn Asp Ala
Asn Asn Gln Ala Lys Ala Thr Phe Asp 530 535 540 Leu Ala Lys Asn Leu
Ala Asp Gly Lys Gly Ala Ala Asp Gly Thr Asn 545 550 555 560 Trp Lys
Ile Asp Asn Lys Val Val Arg Val Pro Tyr Val Gly Val Asp 565 570 575
Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys Gly Ala Gly Thr Gly Gly 580
585 590 Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile
Leu 595 600 605 Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
Val Ser Gly 610 615 620 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu Lys Phe Ile 625 630 635 640 Cys Thr Thr Gly Lys Leu Pro Val
Pro Trp Pro Thr Leu Val Thr Thr 645 650 655 Phe Gly Tyr Gly Leu Gln
Cys Phe Ala Arg Tyr Pro Asp His Met Lys 660 665 670 Gln His Asp Phe
Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 675 680 685 Arg Thr
Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 690 695 700
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 705
710 715 720 Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu
Glu Tyr 725 730 735 Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp
Lys Gln Lys Asn 740 745 750 Gly Ile Lys Val Asn Phe Lys Ile Arg His
Asn Ile Glu Asp Gly Ser 755 760 765 Val Gln Leu Ala Asp His Tyr Gln
Gln Asn Thr Pro Ile Gly Asp Gly 770 775 780 Pro Val Leu Leu Pro Asp
Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu 785 790 795 800 Ser Lys Asp
Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 805 810 815 Val
Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 820 825 830
<210> SEQ ID NO 17 <211> LENGTH: 2496 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: FLIP-mglBF16A-G275S-CFP-K276R-YFP
Vector <220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 17 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc gct gat act cgc att ggt gta aca atc tat aag tac gac 144 Pro
Gly Arg Ala Asp Thr Arg Ile Gly Val Thr Ile Tyr Lys Tyr Asp 35 40
45 gat aac gcg atg tct gta gtg cgc aag gct att gag caa gat gcg aaa
192 Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys
50 55 60 gcc gcg cca gat gtt cag ctg ctg atg aat gat tct cag aat
gac cag 240 Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn
Asp Gln 65 70 75 80 tcc aag cag aac gat cag atc gac gta ttg ctg gcg
aaa ggg gtg aag 288 Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala
Lys Gly Val Lys 85 90 95 gca ctg gca atc aac ctg gtt gac ccg gca
gct gcg ggt acg gtg att 336 Ala Leu Ala Ile Asn Leu Val Asp Pro Ala
Ala Ala Gly Thr Val Ile 100 105 110 gag aaa gcg cgt ggg caa aac gtg
ccg gtg gtt ttc ttc aac aaa gaa 384 Glu Lys Ala Arg Gly Gln Asn Val
Pro Val Val Phe Phe Asn Lys Glu 115 120 125 ccg tct cgt aag gcg ctg
gat agc tac gac aaa gcc tac tac gtt ggc 432 Pro Ser Arg Lys Ala Leu
Asp Ser Tyr Asp Lys Ala Tyr Tyr Val Gly 130 135 140
act gac tcc aaa gag tcc ggc att att caa ggc gat ttg att gct aaa 480
Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp Leu Ile Ala Lys 145
150 155 160 cac tgg gcg gcg aat cag ggt tgg gat ctg aac aaa gac ggt
cag att 528 His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys Asp Gly
Gln Ile 165 170 175 cag ttc gta ctg ctg aaa ggt gaa ccg ggc cat ccg
gat gca gaa gca 576 Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro
Asp Ala Glu Ala 180 185 190 cgt acc act tac gtg att aaa gaa ttg aac
gat aaa ggc atc aaa act 624 Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn
Asp Lys Gly Ile Lys Thr 195 200 205 gaa cag tta cag tta gat acc gca
atg tgg gac acc gct cag gcg aaa 672 Glu Gln Leu Gln Leu Asp Thr Ala
Met Trp Asp Thr Ala Gln Ala Lys 210 215 220 gat aag atg gac gcc tgg
ctg tct ggc ccg aac gcc aac aaa atc gaa 720 Asp Lys Met Asp Ala Trp
Leu Ser Gly Pro Asn Ala Asn Lys Ile Glu 225 230 235 240 gtg gtt atc
gcc aac aac gat gcg atg gca atg ggc gcg gtt gaa gcg 768 Val Val Ile
Ala Asn Asn Asp Ala Met Ala Met Gly Ala Val Glu Ala 245 250 255 ctg
aaa gca cac aac aag tcc agc att ccg gtg ttt ggc gtc gat gcg 816 Leu
Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe Gly Val Asp Ala 260 265
270 ctg cca gaa gcg ctg gcg ctg gtg aaa tcc ggt gca ctg gcg ggc acc
864 Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly Ala Leu Ala Gly Thr
275 280 285 gta ctg aac gat gct aac aac cag gcg aaa gcg acc ttt gat
ctg gcg 912 Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala Thr Phe Asp
Leu Ala 290 295 300 aaa aac ctg gcc gat tcg gct ggt atg gtg agc aag
ggc gag gag ctg 960 Lys Asn Leu Ala Asp Ser Ala Gly Met Val Ser Lys
Gly Glu Glu Leu 305 310 315 320 ttc acc ggg gtg gtg ccc atc ctg gtc
gag ctg gac ggc gac gta aac 1008 Phe Thr Gly Val Val Pro Ile Leu
Val Glu Leu Asp Gly Asp Val Asn 325 330 335 ggc cac aag ttc agc gtg
tcc ggc gag ggc gag ggc gat gcc acc tac 1056 Gly His Lys Phe Ser
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 340 345 350 ggc aag ctg
acc ctg aag ttc atc tgc acc acc ggc aag ctg ccc gtg 1104 Gly Lys
Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 355 360 365
ccc tgg ccc acc ctc gtg acc acc ctg acc tgg ggc gtg cag tgc ttc
1152 Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe 370 375 380 agc cgc tac ccc gac cac atg aag cag cac gac ttc ttc
aag tcc gcc 1200 Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe
Phe Lys Ser Ala 385 390 395 400 atg ccc gaa ggc tac gtc cag gag cgc
acc atc ttc ttc aag gac gac 1248 Met Pro Glu Gly Tyr Val Gln Glu
Arg Thr Ile Phe Phe Lys Asp Asp 405 410 415 ggc aac tac aag acc cgc
gcc gag gtg aag ttc gag ggc gac acc ctg 1296 Gly Asn Tyr Lys Thr
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 420 425 430 gtg aac cgc
atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac 1344 Val Asn
Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 435 440 445
atc ctg ggg cac aag ctg gag tac aac tac atc agc cac aac gtc tat
1392 Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Ile Ser His Asn Val
Tyr 450 455 460 atc acc gcc gac aag cag aag aac ggc atc aag gcc aac
ttc aag atc 1440 Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala
Asn Phe Lys Ile 465 470 475 480 cgc cac aac atc gag gac ggc agc gtg
cag ctc gcc gac cac tac cag 1488 Arg His Asn Ile Glu Asp Gly Ser
Val Gln Leu Ala Asp His Tyr Gln 485 490 495 cag aac acc ccc atc ggc
gac ggc ccc gtg ctg ctg ccc gac aac cac 1536 Gln Asn Thr Pro Ile
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 500 505 510 tac ctg agc
acc cag tcc gcc ctg agc aaa gac ccc aac gag aag cgc 1584 Tyr Leu
Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 515 520 525
gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc
1632 Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr
Leu 530 535 540 ggc atg gac gag ctg tac ggt agc cga ggt gcg gct gat
ggc acc aac 1680 Gly Met Asp Glu Leu Tyr Gly Ser Arg Gly Ala Ala
Asp Gly Thr Asn 545 550 555 560 tgg aaa atc gac aac aaa gtg gtc cgc
gta cct tat gtt ggc gta gat 1728 Trp Lys Ile Asp Asn Lys Val Val
Arg Val Pro Tyr Val Gly Val Asp 565 570 575 aaa gac aac ctg gct gaa
ttc agc aag aaa ggc gcc ggt acc ggt gga 1776 Lys Asp Asn Leu Ala
Glu Phe Ser Lys Lys Gly Ala Gly Thr Gly Gly 580 585 590 atg gtg agc
aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg 1824 Met Val
Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 595 600 605
gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc
1872 Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser
Gly 610 615 620 gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg
aag ttc atc 1920 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr
Leu Lys Phe Ile 625 630 635 640 tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc gtg acc acc 1968 Cys Thr Thr Gly Lys Leu Pro Val
Pro Trp Pro Thr Leu Val Thr Thr 645 650 655 ttc ggc tac ggc ctg cag
tgc ttc gcc cgc tac ccc gac cac atg aag 2016 Phe Gly Tyr Gly Leu
Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 660 665 670 cag cac gac
ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag 2064 Gln His
Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 675 680 685
cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag
2112 Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala
Glu 690 695 700 gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag
ctg aag ggc 2160 Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile
Glu Leu Lys Gly 705 710 715 720 atc gac ttc aag gag gac ggc aac atc
ctg ggg cac aag ctg gag tac 2208 Ile Asp Phe Lys Glu Asp Gly Asn
Ile Leu Gly His Lys Leu Glu Tyr 725 730 735 aac tac aac agc cac aac
gtc tat atc atg gcc gac aag cag aag aac 2256 Asn Tyr Asn Ser His
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 740 745 750 ggc atc aag
gtg aac ttc aag atc cgc cac aac atc gag gac ggc agc 2304 Gly Ile
Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 755 760 765
gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc
2352 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly 770 775 780 ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag
tcc gcc ctg 2400 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr
Gln Ser Ala Leu 785 790 795 800 agc aaa gac ccc aac gag aag cgc gat
cac atg gtc ctg ctg gag ttc 2448 Ser Lys Asp Pro Asn Glu Lys Arg
Asp His Met Val Leu Leu Glu Phe 805 810 815 gtg acc gcc gcc ggg atc
act ctc ggc atg gac gag ctg tac aag taa 2496 Val Thr Ala Ala Gly
Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 820 825 830 <210> SEQ
ID NO 18 <211> LENGTH: 831 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-mglBF16A-G275S-CFP-K276R-YFP Vector
<400> SEQUENCE: 18 Met Arg Gly Ser His His His His His His
Gly Met Ala Ser Met Thr 1 5 10 15 Gly Gly Gln Gln Met Gly Arg Asp
Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 Pro Gly Arg Ala Asp Thr
Arg Ile Gly Val Thr Ile Tyr Lys Tyr Asp 35 40 45 Asp Asn Ala Met
Ser Val Val Arg Lys Ala Ile Glu Gln Asp Ala Lys 50 55 60 Ala Ala
Pro Asp Val Gln Leu Leu Met Asn Asp Ser Gln Asn Asp Gln 65 70 75 80
Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala Lys Gly Val Lys 85
90 95 Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala Gly Thr Val
Ile 100 105 110 Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val Phe Phe
Asn Lys Glu 115 120 125 Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys
Ala Tyr Tyr Val Gly 130 135 140 Thr Asp Ser Lys Glu Ser Gly Ile Ile
Gln Gly Asp Leu Ile Ala Lys 145 150 155 160 His Trp Ala Ala Asn Gln
Gly Trp Asp Leu Asn Lys Asp Gly Gln Ile 165 170 175 Gln Phe Val Leu
Leu Lys Gly Glu Pro Gly His Pro Asp Ala Glu Ala 180 185 190 Arg Thr
Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys Gly Ile Lys Thr 195 200 205
Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr Ala Gln Ala Lys 210
215 220 Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala Asn Lys Ile
Glu 225 230 235 240 Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly
Ala Val Glu Ala 245 250 255 Leu Lys Ala His Asn Lys Ser Ser Ile Pro
Val Phe Gly Val Asp Ala 260 265 270 Leu Pro Glu Ala Leu Ala Leu Val
Lys Ser Gly Ala Leu Ala Gly Thr 275 280 285 Val Leu Asn Asp Ala Asn
Asn Gln Ala Lys Ala Thr Phe Asp Leu Ala 290 295 300 Lys Asn Leu Ala
Asp Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu 305 310 315 320 Phe
Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 325 330
335 Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr
340 345 350 Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu
Pro Val 355 360 365
Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe 370
375 380 Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser
Ala 385 390 395 400 Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe
Phe Lys Asp Asp 405 410 415 Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys
Phe Glu Gly Asp Thr Leu 420 425 430 Val Asn Arg Ile Glu Leu Lys Gly
Ile Asp Phe Lys Glu Asp Gly Asn 435 440 445 Ile Leu Gly His Lys Leu
Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr 450 455 460 Ile Thr Ala Asp
Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 465 470 475 480 Arg
His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 485 490
495 Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His
500 505 510 Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu
Lys Arg 515 520 525 Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala
Gly Ile Thr Leu 530 535 540 Gly Met Asp Glu Leu Tyr Gly Ser Arg Gly
Ala Ala Asp Gly Thr Asn 545 550 555 560 Trp Lys Ile Asp Asn Lys Val
Val Arg Val Pro Tyr Val Gly Val Asp 565 570 575 Lys Asp Asn Leu Ala
Glu Phe Ser Lys Lys Gly Ala Gly Thr Gly Gly 580 585 590 Met Val Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 595 600 605 Val
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 610 615
620 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile
625 630 635 640 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu
Val Thr Thr 645 650 655 Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr
Pro Asp His Met Lys 660 665 670 Gln His Asp Phe Phe Lys Ser Ala Met
Pro Glu Gly Tyr Val Gln Glu 675 680 685 Arg Thr Ile Phe Phe Lys Asp
Asp Gly Asn Tyr Lys Thr Arg Ala Glu 690 695 700 Val Lys Phe Glu Gly
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 705 710 715 720 Ile Asp
Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 725 730 735
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 740
745 750 Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly
Ser 755 760 765 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile
Gly Asp Gly 770 775 780 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser
Tyr Gln Ser Ala Leu 785 790 795 800 Ser Lys Asp Pro Asn Glu Lys Arg
Asp His Met Val Leu Leu Glu Phe 805 810 815 Val Thr Ala Ala Gly Ile
Thr Leu Gly Met Asp Glu Leu Tyr Lys 820 825 830 <210> SEQ ID
NO 19 <211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-Y12S-CFP-D13R Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 19 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tcg gct ggt atg gtg agc aag ggc gag gag ctg ttc acc 912 Ile
Tyr Lys Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr 290 295
300 ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac
960 Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His
305 310 315 320 aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc
tac ggc aag 1008 Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala
Thr Tyr Gly Lys 325 330 335 ctg acc ctg aag ttc atc tgc acc acc ggc
aag ctg ccc gtg ccc tgg 1056 Leu Thr Leu Lys Phe Ile Cys Thr Thr
Gly Lys Leu Pro Val Pro Trp 340 345 350 ccc acc ctc gtg acc acc ctg
acc tgg ggc gtg cag tgc ttc agc cgc 1104 Pro Thr Leu Val Thr Thr
Leu Thr Trp Gly Val Gln Cys Phe Ser Arg 355 360 365 tac ccc gac cac
atg aag cag cac gac ttc ttc aag tcc gcc atg ccc 1152 Tyr Pro Asp
His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 370 375 380 gaa
ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac 1200
Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn 385
390 395 400 tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg
gtg aac 1248 Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr
Leu Val Asn 405 410 415 cgc atc gag ctg aag ggc atc gac ttc aag gag
gac ggc aac atc ctg 1296 Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys
Glu Asp Gly Asn Ile Leu 420 425 430 ggg cac aag ctg gag tac aac tac
atc agc cac aac gtc tat atc acc 1344 Gly His Lys Leu Glu Tyr Asn
Tyr Ile Ser His Asn Val Tyr Ile Thr 435 440 445 gcc gac aag cag aag
aac ggc atc aag gcc aac ttc aag atc cgc cac 1392 Ala Asp Lys Gln
Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His 450 455 460 aac atc
gag gac ggc agc gtg cag ctc gcc gac cac tac cag cag aac 1440 Asn
Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn 465 470
475 480 acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac
ctg 1488 Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His
Tyr Leu 485 490 495 agc acc cag tcc gcc ctg agc aaa gac ccc aac gag
aag cgc gat cac 1536 Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
Glu Lys Arg Asp His 500 505 510 atg gtc ctg ctg gag ttc gtg acc gcc
gcc ggg atc act ctc ggc atg 1584 Met Val Leu Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met 515 520 525 gac gag ctg tac ggt agc
cga gat aac gcg atg tct gta gtg cgc aag 1632 Asp Glu Leu Tyr Gly
Ser Arg Asp Asn Ala Met Ser Val Val Arg Lys 530 535 540 gct att gag
caa gat gcg aaa gcc gcg cca gat gtt cag ctg ctg atg 1680 Ala Ile
Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met 545 550 555
560 aat gat tct cag aat gac cag tcc aag cag aac gat cag atc gac gta
1728 Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp
Val 565 570 575 ttg ctg gcg aaa ggg gtg aag gca ctg gca atc aac ctg
gtt gac ccg 1776 Leu Leu Ala Lys Gly Val Lys Ala Leu Ala Ile Asn
Leu Val Asp Pro 580 585 590 gca gct gcg ggt acg gtg att gag aaa gcg
cgt ggg caa aac gtg ccg 1824 Ala Ala Ala Gly Thr Val Ile Glu Lys
Ala Arg Gly Gln Asn Val Pro 595 600 605
gtg gtt ttc ttc aac aaa gaa ccg tct cgt aag gcg ctg gat agc tac
1872 Val Val Phe Phe Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser
Tyr 610 615 620 gac aaa gcc tac tac gtt ggc act gac tcc aaa gag tcc
ggc att att 1920 Asp Lys Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu
Ser Gly Ile Ile 625 630 635 640 caa ggc gat ttg att gct aaa cac tgg
gcg gcg aat cag ggt tgg gat 1968 Gln Gly Asp Leu Ile Ala Lys His
Trp Ala Ala Asn Gln Gly Trp Asp 645 650 655 ctg aac aaa gac ggt cag
att cag ttc gta ctg ctg aaa ggt gaa ccg 2016 Leu Asn Lys Asp Gly
Gln Ile Gln Phe Val Leu Leu Lys Gly Glu Pro 660 665 670 ggc cat ccg
gat gca gaa gca cgt acc act tac gtg att aaa gaa ttg 2064 Gly His
Pro Asp Ala Glu Ala Arg Thr Thr Tyr Val Ile Lys Glu Leu 675 680 685
aac gat aaa ggc atc aaa act gaa cag tta cag tta gat acc gca atg
2112 Asn Asp Lys Gly Ile Lys Thr Glu Gln Leu Gln Leu Asp Thr Ala
Met 690 695 700 tgg gac acc gct cag gcg aaa gat aag atg gac gcc tgg
ctg tct ggc 2160 Trp Asp Thr Ala Gln Ala Lys Asp Lys Met Asp Ala
Trp Leu Ser Gly 705 710 715 720 ccg aac gcc aac aaa atc gaa gtg gtt
atc gcc aac aac gat gcg atg 2208 Pro Asn Ala Asn Lys Ile Glu Val
Val Ile Ala Asn Asn Asp Ala Met 725 730 735 gca atg ggc gcg gtt gaa
gcg ctg aaa gca cac aac aag tcc agc att 2256 Ala Met Gly Ala Val
Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile 740 745 750 ccg gtg ttt
ggc gtc gat gcg ctg cca gaa gcg ctg gcg ctg gtg aaa 2304 Pro Val
Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys 755 760 765
tcc ggt gca ctg gcg ggc acc gta ctg aac gat gct aac aac cag gcg
2352 Ser Gly Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln
Ala 770 775 780 aaa gcg acc ttt gat ctg gcg aaa aac ctg gcc gat ggt
aaa ggt gcg 2400 Lys Ala Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp
Gly Lys Gly Ala 785 790 795 800 gct gat ggc acc aac tgg aaa atc gac
aac aaa gtg gtc cgc gta cct 2448 Ala Asp Gly Thr Asn Trp Lys Ile
Asp Asn Lys Val Val Arg Val Pro 805 810 815 tat gtt ggc gta gat aaa
gac aac ctg gct gaa ttc agc aag aaa taa 2496 Tyr Val Gly Val Asp
Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ
ID NO 20 <211> LENGTH: 831 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-Y12S-CFP-D13R Vector
<400> SEQUENCE: 20 Met Arg Gly Ser His His His His His His
Gly Met Ala Ser Met Thr 1 5 10 15 Gly Gly Gln Gln Met Gly Arg Asp
Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 Pro Gly Arg Met Val Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40 45 Pro Ile Leu Val
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 50 55 60 Val Ser
Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 65 70 75 80
Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 85
90 95 Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro
Asp 100 105 110 His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr 115 120 125 Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys Thr 130 135 140 Arg Ala Glu Val Lys Phe Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu 145 150 155 160 Leu Lys Gly Ile Asp Phe
Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170 175 Leu Glu Tyr Asn
Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys 180 185 190 Gln Lys
Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu 195 200 205
Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 210
215 220 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr
Gln 225 230 235 240 Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp
His Met Val Leu 245 250 255 Leu Glu Phe Val Thr Ala Ala Gly Ile Thr
Leu Gly Met Asp Glu Leu 260 265 270 Tyr Lys Gly Gly Thr Gly Gly Ala
Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 Ile Tyr Lys Ser Ala Gly
Met Val Ser Lys Gly Glu Glu Leu Phe Thr 290 295 300 Gly Val Val Pro
Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His 305 310 315 320 Lys
Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys 325 330
335 Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp
340 345 350 Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe
Ser Arg 355 360 365 Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys
Ser Ala Met Pro 370 375 380 Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe
Phe Lys Asp Asp Gly Asn 385 390 395 400 Tyr Lys Thr Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn 405 410 415 Arg Ile Glu Leu Lys
Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu 420 425 430 Gly His Lys
Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr 435 440 445 Ala
Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His 450 455
460 Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn
465 470 475 480 Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu 485 490 495 Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn
Glu Lys Arg Asp His 500 505 510 Met Val Leu Leu Glu Phe Val Thr Ala
Ala Gly Ile Thr Leu Gly Met 515 520 525 Asp Glu Leu Tyr Gly Ser Arg
Asp Asn Ala Met Ser Val Val Arg Lys 530 535 540 Ala Ile Glu Gln Asp
Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met 545 550 555 560 Asn Asp
Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp Val 565 570 575
Leu Leu Ala Lys Gly Val Lys Ala Leu Ala Ile Asn Leu Val Asp Pro 580
585 590 Ala Ala Ala Gly Thr Val Ile Glu Lys Ala Arg Gly Gln Asn Val
Pro 595 600 605 Val Val Phe Phe Asn Lys Glu Pro Ser Arg Lys Ala Leu
Asp Ser Tyr 610 615 620 Asp Lys Ala Tyr Tyr Val Gly Thr Asp Ser Lys
Glu Ser Gly Ile Ile 625 630 635 640 Gln Gly Asp Leu Ile Ala Lys His
Trp Ala Ala Asn Gln Gly Trp Asp 645 650 655 Leu Asn Lys Asp Gly Gln
Ile Gln Phe Val Leu Leu Lys Gly Glu Pro 660 665 670 Gly His Pro Asp
Ala Glu Ala Arg Thr Thr Tyr Val Ile Lys Glu Leu 675 680 685 Asn Asp
Lys Gly Ile Lys Thr Glu Gln Leu Gln Leu Asp Thr Ala Met 690 695 700
Trp Asp Thr Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly 705
710 715 720 Pro Asn Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp
Ala Met 725 730 735 Ala Met Gly Ala Val Glu Ala Leu Lys Ala His Asn
Lys Ser Ser Ile 740 745 750 Pro Val Phe Gly Val Asp Ala Leu Pro Glu
Ala Leu Ala Leu Val Lys 755 760 765 Ser Gly Ala Leu Ala Gly Thr Val
Leu Asn Asp Ala Asn Asn Gln Ala 770 775 780 Lys Ala Thr Phe Asp Leu
Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala 785 790 795 800 Ala Asp Gly
Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val Pro 805 810 815 Tyr
Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys 820 825 830
<210> SEQ ID NO 21 <211> LENGTH: 2496 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: FLIP-YFP-mglBF16A-P32S-CFP-D33R
Vector <220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 21 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60
gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg 240
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 65
70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc
acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro
Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc ttc agc
cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser
Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc aag tcc
gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe Lys Ser
Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc ttc ttc
aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile Phe Phe
Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg aag ttc
gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val Lys Phe
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg aag ggc
atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu Lys Gly
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170 175 ctg
gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag 576 Leu
Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys 180 185
190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag
624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu
195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag aac acc
ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr
Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac
ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr
Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc aac gag
aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro Asn Glu
Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc gcc gcc
ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr Ala Ala
Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt ggt acc
gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly Gly Thr
Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc tat aag
tac gac gat aac gcg atg tct gta gtg cgc aag gct att 912 Ile Tyr Lys
Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295 300 gag
caa gat gcg aaa gcc gcg tcg gct ggt atg gtg agc aag ggc gag 960 Glu
Gln Asp Ala Lys Ala Ala Ser Ala Gly Met Val Ser Lys Gly Glu 305 310
315 320 gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc
gac 1008 Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp
Gly Asp 325 330 335 gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc
gag ggc gat gcc 1056 Val Asn Gly His Lys Phe Ser Val Ser Gly Glu
Gly Glu Gly Asp Ala 340 345 350 acc tac ggc aag ctg acc ctg aag ttc
atc tgc acc acc ggc aag ctg 1104 Thr Tyr Gly Lys Leu Thr Leu Lys
Phe Ile Cys Thr Thr Gly Lys Leu 355 360 365 ccc gtg ccc tgg ccc acc
ctc gtg acc acc ctg acc tgg ggc gtg cag 1152 Pro Val Pro Trp Pro
Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln 370 375 380 tgc ttc agc
cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag 1200 Cys Phe
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 385 390 395
400 tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag
1248 Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe
Lys 405 410 415 gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc
gag ggc gac 1296 Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys
Phe Glu Gly Asp 420 425 430 acc ctg gtg aac cgc atc gag ctg aag ggc
atc gac ttc aag gag gac 1344 Thr Leu Val Asn Arg Ile Glu Leu Lys
Gly Ile Asp Phe Lys Glu Asp 435 440 445 ggc aac atc ctg ggg cac aag
ctg gag tac aac tac atc agc cac aac 1392 Gly Asn Ile Leu Gly His
Lys Leu Glu Tyr Asn Tyr Ile Ser His Asn 450 455 460 gtc tat atc acc
gcc gac aag cag aag aac ggc atc aag gcc aac ttc 1440 Val Tyr Ile
Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe 465 470 475 480
aag atc cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac cac
1488 Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp
His 485 490 495 tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg
ctg ccc gac 1536 Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val
Leu Leu Pro Asp 500 505 510 aac cac tac ctg agc acc cag tcc gcc ctg
agc aaa gac ccc aac gag 1584 Asn His Tyr Leu Ser Thr Gln Ser Ala
Leu Ser Lys Asp Pro Asn Glu 515 520 525 aag cgc gat cac atg gtc ctg
ctg gag ttc gtg acc gcc gcc ggg atc 1632 Lys Arg Asp His Met Val
Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 530 535 540 act ctc ggc atg
gac gag ctg tac ggt agc cga gtt cag ctg ctg atg 1680 Thr Leu Gly
Met Asp Glu Leu Tyr Gly Ser Arg Val Gln Leu Leu Met 545 550 555 560
aat gat tct cag aat gac cag tcc aag cag aac gat cag atc gac gta
1728 Asn Asp Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp
Val 565 570 575 ttg ctg gcg aaa ggg gtg aag gca ctg gca atc aac ctg
gtt gac ccg 1776 Leu Leu Ala Lys Gly Val Lys Ala Leu Ala Ile Asn
Leu Val Asp Pro 580 585 590 gca gct gcg ggt acg gtg att gag aaa gcg
cgt ggg caa aac gtg ccg 1824 Ala Ala Ala Gly Thr Val Ile Glu Lys
Ala Arg Gly Gln Asn Val Pro 595 600 605 gtg gtt ttc ttc aac aaa gaa
ccg tct cgt aag gcg ctg gat agc tac 1872 Val Val Phe Phe Asn Lys
Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr 610 615 620 gac aaa gcc tac
tac gtt ggc act gac tcc aaa gag tcc ggc att att 1920 Asp Lys Ala
Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile 625 630 635 640
caa ggc gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
1968 Gln Gly Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp 645 650 655 ctg aac aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg 2016 Leu Asn Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro 660 665 670 ggc cat ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg 2064 Gly His Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu 675 680 685 aac gat aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg 2112 Asn Asp Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met 690 695 700 tgg gac acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc 2160 Trp Asp Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly 705 710 715 720
ccg aac gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg
2208 Pro Asn Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala
Met 725 730 735 gca atg ggc gcg gtt gaa gcg ctg aaa gca cac aac aag
tcc agc att 2256 Ala Met Gly Ala Val Glu Ala Leu Lys Ala His Asn
Lys Ser Ser Ile 740 745 750 ccg gtg ttt ggc gtc gat gcg ctg cca gaa
gcg ctg gcg ctg gtg aaa 2304 Pro Val Phe Gly Val Asp Ala Leu Pro
Glu Ala Leu Ala Leu Val Lys 755 760 765 tcc ggt gca ctg gcg ggc acc
gta ctg aac gat gct aac aac cag gcg 2352 Ser Gly Ala Leu Ala Gly
Thr Val Leu Asn Asp Ala Asn Asn Gln Ala 770 775 780 aaa gcg acc ttt
gat ctg gcg aaa aac ctg gcc gat ggt aaa ggt gcg 2400 Lys Ala Thr
Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala 785 790 795 800
gct gat ggc acc aac tgg aaa atc gac aac aaa gtg gtc cgc gta cct
2448 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro 805 810 815 tat gtt ggc gta gat aaa gac aac ctg gct gaa ttc agc
aag aaa taa 2496 Tyr Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys 820 825 830 <210> SEQ ID NO 22 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-YFP-mglBF16A-P32S-CFP-D33R Vector <400> SEQUENCE: 22 Met
Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5 10
15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp
20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly
His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr
Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile Cys Thr Thr Gly Lys
Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val Thr Thr Leu Thr Trp
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105 110 His Met Lys Gln
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 Val Gln
Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145
150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile
Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys
Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser Val Gln Leu Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly Asp Gly Pro Val Leu
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230 235 240 Ser Ala Leu
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 245 250 255
Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260
265 270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val
Thr 275 280 285 Ile Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg
Lys Ala Ile 290 295 300 Glu Gln Asp Ala Lys Ala Ala Ser Ala Gly Met
Val Ser Lys Gly Glu 305 310 315 320 Glu Leu Phe Thr Gly Val Val Pro
Ile Leu Val Glu Leu Asp Gly Asp 325 330 335 Val Asn Gly His Lys Phe
Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 340 345 350 Thr Tyr Gly Lys
Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 355 360 365 Pro Val
Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln 370 375 380
Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 385
390 395 400 Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe
Phe Lys 405 410 415 Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys
Phe Glu Gly Asp 420 425 430 Thr Leu Val Asn Arg Ile Glu Leu Lys Gly
Ile Asp Phe Lys Glu Asp 435 440 445 Gly Asn Ile Leu Gly His Lys Leu
Glu Tyr Asn Tyr Ile Ser His Asn 450 455 460 Val Tyr Ile Thr Ala Asp
Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe 465 470 475 480 Lys Ile Arg
His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 485 490 495 Tyr
Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 500 505
510 Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu
515 520 525 Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala
Gly Ile 530 535 540 Thr Leu Gly Met Asp Glu Leu Tyr Gly Ser Arg Val
Gln Leu Leu Met 545 550 555 560 Asn Asp Ser Gln Asn Asp Gln Ser Lys
Gln Asn Asp Gln Ile Asp Val 565 570 575 Leu Leu Ala Lys Gly Val Lys
Ala Leu Ala Ile Asn Leu Val Asp Pro 580 585 590 Ala Ala Ala Gly Thr
Val Ile Glu Lys Ala Arg Gly Gln Asn Val Pro 595 600 605 Val Val Phe
Phe Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr 610 615 620 Asp
Lys Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile 625 630
635 640 Gln Gly Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp 645 650 655 Leu Asn Lys Asp Gly Gln Ile Gln Phe Val Leu Leu Lys
Gly Glu Pro 660 665 670 Gly His Pro Asp Ala Glu Ala Arg Thr Thr Tyr
Val Ile Lys Glu Leu 675 680 685 Asn Asp Lys Gly Ile Lys Thr Glu Gln
Leu Gln Leu Asp Thr Ala Met 690 695 700 Trp Asp Thr Ala Gln Ala Lys
Asp Lys Met Asp Ala Trp Leu Ser Gly 705 710 715 720 Pro Asn Ala Asn
Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met 725 730 735 Ala Met
Gly Ala Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile 740 745 750
Pro Val Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys 755
760 765 Ser Gly Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln
Ala 770 775 780 Lys Ala Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly
Lys Gly Ala 785 790 795 800 Ala Asp Gly Thr Asn Trp Lys Ile Asp Asn
Lys Val Val Arg Val Pro 805 810 815 Tyr Val Gly Val Asp Lys Asp Asn
Leu Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ ID NO 23
<211> LENGTH: 2496 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-G275S-CFP-K276R Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 23 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tac gac gat aac gcg atg tct gta gtg cgc aag gct att 912 Ile
Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295
300 gag caa gat gcg aaa gcc gcg cca gat gtt cag ctg ctg atg aat gat
960 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp
305 310 315 320 tct cag aat gac cag tcc aag cag aac gat cag atc gac
gta ttg ctg 1008 Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile
Asp Val Leu Leu 325 330 335 gcg aaa ggg gtg aag gca ctg gca atc aac
ctg gtt gac ccg gca gct 1056 Ala Lys Gly Val Lys Ala Leu Ala Ile
Asn Leu Val Asp Pro Ala Ala 340 345 350 gcg ggt acg gtg att gag aaa
gcg cgt ggg caa aac gtg ccg gtg gtt 1104 Ala Gly Thr Val Ile Glu
Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 ttc ttc aac aaa
gaa ccg tct cgt aag gcg ctg gat agc tac gac aaa 1152 Phe Phe Asn
Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380 gcc
tac tac gtt ggc act gac tcc aaa gag tcc ggc att att caa ggc 1200
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385
390 395 400 gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
ctg aac 1248 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp Leu Asn 405 410 415 aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg ggc cat 1296 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro Gly His 420 425 430 ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg aac gat 1344 Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu Asn Asp 435 440 445 aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg tgg gac 1392 Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp 450 455 460 acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc ccg aac 1440 Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470
475 480 gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg gca
atg 1488 Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met
Ala Met 485 490 495 ggc gcg gtt gaa gcg ctg aaa gca cac aac aag tcc
agc att ccg gtg 1536 Gly Ala Val Glu Ala Leu Lys Ala His Asn Lys
Ser Ser Ile Pro Val 500 505 510 ttt ggc gtc gat gcg ctg cca gaa gcg
ctg gcg ctg gtg aaa tcc ggt 1584 Phe Gly Val Asp Ala Leu Pro Glu
Ala Leu Ala Leu Val Lys Ser Gly
515 520 525 gca ctg gcg ggc acc gta ctg aac gat gct aac aac cag gcg
aaa gcg 1632 Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln
Ala Lys Ala 530 535 540 acc ttt gat ctg gcg aaa aac ctg gcc gat tcg
gct ggt atg gtg agc 1680 Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp
Ser Ala Gly Met Val Ser 545 550 555 560 aag ggc gag gag ctg ttc acc
ggg gtg gtg ccc atc ctg gtc gag ctg 1728 Lys Gly Glu Glu Leu Phe
Thr Gly Val Val Pro Ile Leu Val Glu Leu 565 570 575 gac ggc gac gta
aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag 1776 Asp Gly Asp
Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 580 585 590 ggc
gat gcc acc tac ggc aag ctg acc ctg aag ttc atc tgc acc acc 1824
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 595
600 605 ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg acc
tgg 1872 Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu
Thr Trp 610 615 620 ggc gtg cag tgc ttc agc cgc tac ccc gac cac atg
aag cag cac gac 1920 Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His
Met Lys Gln His Asp 625 630 635 640 ttc ttc aag tcc gcc atg ccc gaa
ggc tac gtc cag gag cgc acc atc 1968 Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr Val Gln Glu Arg Thr Ile 645 650 655 ttc ttc aag gac gac
ggc aac tac aag acc cgc gcc gag gtg aag ttc 2016 Phe Phe Lys Asp
Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 660 665 670 gag ggc
gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc 2064 Glu
Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 675 680
685 aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac atc
2112 Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr
Ile 690 695 700 agc cac aac gtc tat atc acc gcc gac aag cag aag aac
ggc atc aag 2160 Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys
Asn Gly Ile Lys 705 710 715 720 gcc aac ttc aag atc cgc cac aac atc
gag gac ggc agc gtg cag ctc 2208 Ala Asn Phe Lys Ile Arg His Asn
Ile Glu Asp Gly Ser Val Gln Leu 725 730 735 gcc gac cac tac cag cag
aac acc ccc atc ggc gac ggc ccc gtg ctg 2256 Ala Asp His Tyr Gln
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 740 745 750 ctg ccc gac
aac cac tac ctg agc acc cag tcc gcc ctg agc aaa gac 2304 Leu Pro
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 755 760 765
ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc
2352 Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr
Ala 770 775 780 gcc ggg atc act ctc ggc atg gac gag ctg tac ggt agc
cga ggt gcg 2400 Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Gly
Ser Arg Gly Ala 785 790 795 800 gct gat ggc acc aac tgg aaa atc gac
aac aaa gtg gtc cgc gta cct 2448 Ala Asp Gly Thr Asn Trp Lys Ile
Asp Asn Lys Val Val Arg Val Pro 805 810 815 tat gtt ggc gta gat aaa
gac aac ctg gct gaa ttc agc aag aaa taa 2496 Tyr Val Gly Val Asp
Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ
ID NO 24 <211> LENGTH: 831 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: FLIP-YFP-mglBF16A-G275S-CFP-K276R Vector
<400> SEQUENCE: 24 Met Arg Gly Ser His His His His His His
Gly Met Ala Ser Met Thr 1 5 10 15 Gly Gly Gln Gln Met Gly Arg Asp
Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 Pro Gly Arg Met Val Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40 45 Pro Ile Leu Val
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 50 55 60 Val Ser
Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 65 70 75 80
Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 85
90 95 Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro
Asp 100 105 110 His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr 115 120 125 Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys Thr 130 135 140 Arg Ala Glu Val Lys Phe Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu 145 150 155 160 Leu Lys Gly Ile Asp Phe
Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170 175 Leu Glu Tyr Asn
Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys 180 185 190 Gln Lys
Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu 195 200 205
Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 210
215 220 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr
Gln 225 230 235 240 Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp
His Met Val Leu 245 250 255 Leu Glu Phe Val Thr Ala Ala Gly Ile Thr
Leu Gly Met Asp Glu Leu 260 265 270 Tyr Lys Gly Gly Thr Gly Gly Ala
Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 Ile Tyr Lys Tyr Asp Asp
Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295 300 Glu Gln Asp Ala
Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp 305 310 315 320 Ser
Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu 325 330
335 Ala Lys Gly Val Lys Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala
340 345 350 Ala Gly Thr Val Ile Glu Lys Ala Arg Gly Gln Asn Val Pro
Val Val 355 360 365 Phe Phe Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp
Ser Tyr Asp Lys 370 375 380 Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu
Ser Gly Ile Ile Gln Gly 385 390 395 400 Asp Leu Ile Ala Lys His Trp
Ala Ala Asn Gln Gly Trp Asp Leu Asn 405 410 415 Lys Asp Gly Gln Ile
Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His 420 425 430 Pro Asp Ala
Glu Ala Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp 435 440 445 Lys
Gly Ile Lys Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp 450 455
460 Thr Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn
465 470 475 480 Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala
Met Ala Met 485 490 495 Gly Ala Val Glu Ala Leu Lys Ala His Asn Lys
Ser Ser Ile Pro Val 500 505 510 Phe Gly Val Asp Ala Leu Pro Glu Ala
Leu Ala Leu Val Lys Ser Gly 515 520 525 Ala Leu Ala Gly Thr Val Leu
Asn Asp Ala Asn Asn Gln Ala Lys Ala 530 535 540 Thr Phe Asp Leu Ala
Lys Asn Leu Ala Asp Ser Ala Gly Met Val Ser 545 550 555 560 Lys Gly
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 565 570 575
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 580
585 590 Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr
Thr 595 600 605 Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr
Leu Thr Trp 610 615 620 Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His
Met Lys Gln His Asp 625 630 635 640 Phe Phe Lys Ser Ala Met Pro Glu
Gly Tyr Val Gln Glu Arg Thr Ile 645 650 655 Phe Phe Lys Asp Asp Gly
Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 660 665 670 Glu Gly Asp Thr
Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 675 680 685 Lys Glu
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Ile 690 695 700
Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys 705
710 715 720 Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val
Gln Leu 725 730 735 Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp
Gly Pro Val Leu 740 745 750 Leu Pro Asp Asn His Tyr Leu Ser Thr Gln
Ser Ala Leu Ser Lys Asp 755 760 765 Pro Asn Glu Lys Arg Asp His Met
Val Leu Leu Glu Phe Val Thr Ala 770 775 780 Ala Gly Ile Thr Leu Gly
Met Asp Glu Leu Tyr Gly Ser Arg Gly Ala 785 790 795 800 Ala Asp Gly
Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val Pro 805 810 815 Tyr
Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys 820 825 830
<210> SEQ ID NO 25 <211> LENGTH: 2496 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: FLIP-YFP-mglBF16A-T282S-CFP-N283R
Vector
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(2496) <400> SEQUENCE: 25 atg cgg ggt tct cat
cat cat cat cat cat ggt atg gct agc atg act 48 Met Arg Gly Ser His
His His His His His Gly Met Ala Ser Met Thr 1 5 10 15 ggt gga cag
caa atg ggt cgg gat ctg tac gac gat gac gat aag gat 96 Gly Gly Gln
Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 20 25 30 ccg
ggc cgc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg 144 Pro
Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 35 40
45 ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc
192 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
50 55 60 gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg
acc ctg 240 Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu
Thr Leu 65 70 75 80 aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc
tgg ccc acc ctc 288 Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro
Trp Pro Thr Leu 85 90 95 gtg acc acc ctg acc tgg ggc gtg cag tgc
ttc agc cgc tac ccc gac 336 Val Thr Thr Leu Thr Trp Gly Val Gln Cys
Phe Ser Arg Tyr Pro Asp 100 105 110 cac atg aag cag cac gac ttc ttc
aag tcc gcc atg ccc gaa ggc tac 384 His Met Lys Gln His Asp Phe Phe
Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 gtc cag gag cgc acc atc
ttc ttc aag gac gac ggc aac tac aag acc 432 Val Gln Glu Arg Thr Ile
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135 140 cgc gcc gag gtg
aag ttc gag ggc gac acc ctg gtg aac cgc atc gag 480 Arg Ala Glu Val
Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145 150 155 160 ctg
aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag 528 Leu
Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 165 170
175 ctg gag tac aac tac atc agc cac aac gtc tat atc acc gcc gac aag
576 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp Lys
180 185 190 cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac
atc gag 624 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile Glu 195 200 205 gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc atc 672 Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln
Asn Thr Pro Ile 210 215 220 ggc gac ggc ccc gtg ctg ctg ccc gac aac
cac tac ctg agc acc cag 720 Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
His Tyr Leu Ser Thr Gln 225 230 235 240 tcc gcc ctg agc aaa gac ccc
aac gag aag cgc gat cac atg gtc ctg 768 Ser Ala Leu Ser Lys Asp Pro
Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 ctg gag ttc gtg acc
gcc gcc ggg atc act ctc ggc atg gac gag ctg 816 Leu Glu Phe Val Thr
Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265 270 tac aag ggt
ggt acc gga ggc gcc gct gat act cgc att ggt gta aca 864 Tyr Lys Gly
Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr 275 280 285 atc
tat aag tac gac gat aac gcg atg tct gta gtg cgc aag gct att 912 Ile
Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys Ala Ile 290 295
300 gag caa gat gcg aaa gcc gcg cca gat gtt cag ctg ctg atg aat gat
960 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp
305 310 315 320 tct cag aat gac cag tcc aag cag aac gat cag atc gac
gta ttg ctg 1008 Ser Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile
Asp Val Leu Leu 325 330 335 gcg aaa ggg gtg aag gca ctg gca atc aac
ctg gtt gac ccg gca gct 1056 Ala Lys Gly Val Lys Ala Leu Ala Ile
Asn Leu Val Asp Pro Ala Ala 340 345 350 gcg ggt acg gtg att gag aaa
gcg cgt ggg caa aac gtg ccg gtg gtt 1104 Ala Gly Thr Val Ile Glu
Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 ttc ttc aac aaa
gaa ccg tct cgt aag gcg ctg gat agc tac gac aaa 1152 Phe Phe Asn
Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380 gcc
tac tac gtt ggc act gac tcc aaa gag tcc ggc att att caa ggc 1200
Ala Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385
390 395 400 gat ttg att gct aaa cac tgg gcg gcg aat cag ggt tgg gat
ctg aac 1248 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp
Asp Leu Asn 405 410 415 aaa gac ggt cag att cag ttc gta ctg ctg aaa
ggt gaa ccg ggc cat 1296 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu
Lys Gly Glu Pro Gly His 420 425 430 ccg gat gca gaa gca cgt acc act
tac gtg att aaa gaa ttg aac gat 1344 Pro Asp Ala Glu Ala Arg Thr
Thr Tyr Val Ile Lys Glu Leu Asn Asp 435 440 445 aaa ggc atc aaa act
gaa cag tta cag tta gat acc gca atg tgg gac 1392 Lys Gly Ile Lys
Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp 450 455 460 acc gct
cag gcg aaa gat aag atg gac gcc tgg ctg tct ggc ccg aac 1440 Thr
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470
475 480 gcc aac aaa atc gaa gtg gtt atc gcc aac aac gat gcg atg gca
atg 1488 Ala Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met
Ala Met 485 490 495 ggc gcg gtt gaa gcg ctg aaa gca cac aac aag tcc
agc att ccg gtg 1536 Gly Ala Val Glu Ala Leu Lys Ala His Asn Lys
Ser Ser Ile Pro Val 500 505 510 ttt ggc gtc gat gcg ctg cca gaa gcg
ctg gcg ctg gtg aaa tcc ggt 1584 Phe Gly Val Asp Ala Leu Pro Glu
Ala Leu Ala Leu Val Lys Ser Gly 515 520 525 gca ctg gcg ggc acc gta
ctg aac gat gct aac aac cag gcg aaa gcg 1632 Ala Leu Ala Gly Thr
Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala 530 535 540 acc ttt gat
ctg gcg aaa aac ctg gcc gat ggt aaa ggt gcg gct gat 1680 Thr Phe
Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala Ala Asp 545 550 555
560 ggc tcg gct ggt atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg
1728 Gly Ser Ala Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val 565 570 575 gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc
cac aag ttc 1776 Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn
Gly His Lys Phe 580 585 590 agc gtg tcc ggc gag ggc gag ggc gat gcc
acc tac ggc aag ctg acc 1824 Ser Val Ser Gly Glu Gly Glu Gly Asp
Ala Thr Tyr Gly Lys Leu Thr 595 600 605 ctg aag ttc atc tgc acc acc
ggc aag ctg ccc gtg ccc tgg ccc acc 1872 Leu Lys Phe Ile Cys Thr
Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 610 615 620 ctc gtg acc acc
ctg acc tgg ggc gtg cag tgc ttc agc cgc tac ccc 1920 Leu Val Thr
Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro 625 630 635 640
gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc
1968 Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu
Gly 645 650 655 tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc
aac tac aag 2016 Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp
Gly Asn Tyr Lys 660 665 670 acc cgc gcc gag gtg aag ttc gag ggc gac
acc ctg gtg aac cgc atc 2064 Thr Arg Ala Glu Val Lys Phe Glu Gly
Asp Thr Leu Val Asn Arg Ile 675 680 685 gag ctg aag ggc atc gac ttc
aag gag gac ggc aac atc ctg ggg cac 2112 Glu Leu Lys Gly Ile Asp
Phe Lys Glu Asp Gly Asn Ile Leu Gly His 690 695 700 aag ctg gag tac
aac tac atc agc cac aac gtc tat atc acc gcc gac 2160 Lys Leu Glu
Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile Thr Ala Asp 705 710 715 720
aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc
2208 Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn
Ile 725 730 735 gag gac ggc agc gtg cag ctc gcc gac cac tac cag cag
aac acc ccc 2256 Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln
Gln Asn Thr Pro 740 745 750 atc ggc gac ggc ccc gtg ctg ctg ccc gac
aac cac tac ctg agc acc 2304 Ile Gly Asp Gly Pro Val Leu Leu Pro
Asp Asn His Tyr Leu Ser Thr 755 760 765 cag tcc gcc ctg agc aaa gac
ccc aac gag aag cgc gat cac atg gtc 2352 Gln Ser Ala Leu Ser Lys
Asp Pro Asn Glu Lys Arg Asp His Met Val 770 775 780 ctg ctg gag ttc
gtg acc gcc gcc ggg atc act ctc ggc atg gac gag 2400 Leu Leu Glu
Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu 785 790 795 800
ctg tac ggt agc cga tgg aaa atc gac aac aaa gtg gtc cgc gta cct
2448 Leu Tyr Gly Ser Arg Trp Lys Ile Asp Asn Lys Val Val Arg Val
Pro 805 810 815 tat gtt ggc gta gat aaa gac aac ctg gct gaa ttc agc
aag aaa taa 2496 Tyr Val Gly Val Asp Lys Asp Asn Leu Ala Glu Phe
Ser Lys Lys 820 825 830 <210> SEQ ID NO 26 <211>
LENGTH: 831 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
FLIP-YFP-mglBF16A-T282S-CFP-N283R Vector <400> SEQUENCE: 26
Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 1 5
10 15 Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys
Asp 20 25 30 Pro Gly Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr
Gly Val Val 35 40 45 Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn
Gly His Lys Phe Ser 50 55 60 Val Ser Gly Glu Gly Glu Gly Asp Ala
Thr Tyr Gly Lys Leu Thr Leu 65 70 75 80 Lys Phe Ile Cys Thr Thr Gly
Lys Leu Pro Val Pro Trp Pro Thr Leu 85 90 95 Val Thr Thr Leu Thr
Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 100 105 110 His Met Lys
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 115 120 125 Val
Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 130 135
140
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 145
150 155 160 Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
His Lys 165 170 175 Leu Glu Tyr Asn Tyr Ile Ser His Asn Val Tyr Ile
Thr Ala Asp Lys 180 185 190 Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys
Ile Arg His Asn Ile Glu 195 200 205 Asp Gly Ser Val Gln Leu Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile 210 215 220 Gly Asp Gly Pro Val Leu
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 225 230 235 240 Ser Ala Leu
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 245 250 255 Leu
Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 260 265
270 Tyr Lys Gly Gly Thr Gly Gly Ala Ala Asp Thr Arg Ile Gly Val Thr
275 280 285 Ile Tyr Lys Tyr Asp Asp Asn Ala Met Ser Val Val Arg Lys
Ala Ile 290 295 300 Glu Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu
Leu Met Asn Asp 305 310 315 320 Ser Gln Asn Asp Gln Ser Lys Gln Asn
Asp Gln Ile Asp Val Leu Leu 325 330 335 Ala Lys Gly Val Lys Ala Leu
Ala Ile Asn Leu Val Asp Pro Ala Ala 340 345 350 Ala Gly Thr Val Ile
Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val 355 360 365 Phe Phe Asn
Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys 370 375 380 Ala
Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly 385 390
395 400 Asp Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp Leu
Asn 405 410 415 Lys Asp Gly Gln Ile Gln Phe Val Leu Leu Lys Gly Glu
Pro Gly His 420 425 430 Pro Asp Ala Glu Ala Arg Thr Thr Tyr Val Ile
Lys Glu Leu Asn Asp 435 440 445 Lys Gly Ile Lys Thr Glu Gln Leu Gln
Leu Asp Thr Ala Met Trp Asp 450 455 460 Thr Ala Gln Ala Lys Asp Lys
Met Asp Ala Trp Leu Ser Gly Pro Asn 465 470 475 480 Ala Asn Lys Ile
Glu Val Val Ile Ala Asn Asn Asp Ala Met Ala Met 485 490 495 Gly Ala
Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val 500 505 510
Phe Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly 515
520 525 Ala Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala Lys
Ala 530 535 540 Thr Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly
Ala Ala Asp 545 550 555 560 Gly Ser Ala Gly Met Val Ser Lys Gly Glu
Glu Leu Phe Thr Gly Val 565 570 575 Val Pro Ile Leu Val Glu Leu Asp
Gly Asp Val Asn Gly His Lys Phe 580 585 590 Ser Val Ser Gly Glu Gly
Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr 595 600 605 Leu Lys Phe Ile
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 610 615 620 Leu Val
Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ser Arg Tyr Pro 625 630 635
640 Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly
645 650 655 Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn
Tyr Lys 660 665 670 Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu
Val Asn Arg Ile 675 680 685 Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp
Gly Asn Ile Leu Gly His 690 695 700 Lys Leu Glu Tyr Asn Tyr Ile Ser
His Asn Val Tyr Ile Thr Ala Asp 705 710 715 720 Lys Gln Lys Asn Gly
Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile 725 730 735 Glu Asp Gly
Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 740 745 750 Ile
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr 755 760
765 Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val
770 775 780 Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met
Asp Glu 785 790 795 800 Leu Tyr Gly Ser Arg Trp Lys Ile Asp Asn Lys
Val Val Arg Val Pro 805 810 815 Tyr Val Gly Val Asp Lys Asp Asn Leu
Ala Glu Phe Ser Lys Lys 820 825 830 <210> SEQ ID NO 27
<211> LENGTH: 909 <212> TYPE: DNA <213> ORGANISM:
Escherichia coli K12 <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(909) <400> SEQUENCE: 27 atg caa
tta cgt aaa cct gcc aca gca atc ctc gcc ctg gcg ctt tcc 48 Met Gln
Leu Arg Lys Pro Ala Thr Ala Ile Leu Ala Leu Ala Leu Ser 1 5 10 15
gca gga ctg gca cag gca gat gac gcc gcc ccg gca gcg ggc agt act 96
Ala Gly Leu Ala Gln Ala Asp Asp Ala Ala Pro Ala Ala Gly Ser Thr 20
25 30 ctg gac aaa atc gcc aaa aac ggt gtg att gtc gtc ggt cac cgt
gaa 144 Leu Asp Lys Ile Ala Lys Asn Gly Val Ile Val Val Gly His Arg
Glu 35 40 45 tct tca gtg cct ttc tct tat tac gac aat cag caa aaa
gtg gtg ggt 192 Ser Ser Val Pro Phe Ser Tyr Tyr Asp Asn Gln Gln Lys
Val Val Gly 50 55 60 tac tcg cag gat tac tcc aac gcc att gtt gaa
gca gtg aaa aag aaa 240 Tyr Ser Gln Asp Tyr Ser Asn Ala Ile Val Glu
Ala Val Lys Lys Lys 65 70 75 80 ctc aac aaa ccg gac ttg cag gta aaa
ctg att ccg att acc tca caa 288 Leu Asn Lys Pro Asp Leu Gln Val Lys
Leu Ile Pro Ile Thr Ser Gln 85 90 95 aac cgt att cca ctg ctg caa
aac ggc act ttc gat ttt gaa tgt ggt 336 Asn Arg Ile Pro Leu Leu Gln
Asn Gly Thr Phe Asp Phe Glu Cys Gly 100 105 110 tct acc acc aac aac
gtc gaa cgc caa aaa cag gcg gct ttc tct gac 384 Ser Thr Thr Asn Asn
Val Glu Arg Gln Lys Gln Ala Ala Phe Ser Asp 115 120 125 act att ttc
gtg gtc ggt acg cgc ctg ttg acc aaa aag ggt ggc gat 432 Thr Ile Phe
Val Val Gly Thr Arg Leu Leu Thr Lys Lys Gly Gly Asp 130 135 140 atc
aaa gat ttt gcc aac ctg aaa gac aaa gcc gta gtc gtc act tcc 480 Ile
Lys Asp Phe Ala Asn Leu Lys Asp Lys Ala Val Val Val Thr Ser 145 150
155 160 ggc act acc tct gaa gtt ttg ctc aac aaa ctg aat gaa gag caa
aaa 528 Gly Thr Thr Ser Glu Val Leu Leu Asn Lys Leu Asn Glu Glu Gln
Lys 165 170 175 atg aat atg cgc atc atc agc gcc aaa gat cac ggt gac
tct ttc cgc 576 Met Asn Met Arg Ile Ile Ser Ala Lys Asp His Gly Asp
Ser Phe Arg 180 185 190 acc ctg gaa agc ggt cgt gcc gtt gcc ttt atg
atg gat gac gct ctg 624 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe Met
Met Asp Asp Ala Leu 195 200 205 ctg gcc ggt gaa cgt gcg aaa gcg aag
aaa cca gac aac tgg gaa atc 672 Leu Ala Gly Glu Arg Ala Lys Ala Lys
Lys Pro Asp Asn Trp Glu Ile 210 215 220 gtc ggc aag ccg cag tct cag
gag gcc tac ggt tgt atg ttg cgt aaa 720 Val Gly Lys Pro Gln Ser Gln
Glu Ala Tyr Gly Cys Met Leu Arg Lys 225 230 235 240 gat gat ccg cag
ttc aaa aag ctg atg gat gac acc atc gct cag gtg 768 Asp Asp Pro Gln
Phe Lys Lys Leu Met Asp Asp Thr Ile Ala Gln Val 245 250 255 cag acc
tcc ggt gaa gcg gaa aaa tgg ttt gat aag tgg ttc aaa aat 816 Gln Thr
Ser Gly Glu Ala Glu Lys Trp Phe Asp Lys Trp Phe Lys Asn 260 265 270
cca att ccg ccg aaa aac ctg aac atg aat ttc gaa ctg tca gac gaa 864
Pro Ile Pro Pro Lys Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu 275
280 285 atg aaa gca ctg ttc aaa gaa ccg aat gac aag gca ctg aac taa
909 Met Lys Ala Leu Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn 290 295
300 <210> SEQ ID NO 28 <211> LENGTH: 302 <212>
TYPE: PRT <213> ORGANISM: Escherichia coli K12 <400>
SEQUENCE: 28 Met Gln Leu Arg Lys Pro Ala Thr Ala Ile Leu Ala Leu
Ala Leu Ser 1 5 10 15 Ala Gly Leu Ala Gln Ala Asp Asp Ala Ala Pro
Ala Ala Gly Ser Thr 20 25 30 Leu Asp Lys Ile Ala Lys Asn Gly Val
Ile Val Val Gly His Arg Glu 35 40 45 Ser Ser Val Pro Phe Ser Tyr
Tyr Asp Asn Gln Gln Lys Val Val Gly 50 55 60 Tyr Ser Gln Asp Tyr
Ser Asn Ala Ile Val Glu Ala Val Lys Lys Lys 65 70 75 80 Leu Asn Lys
Pro Asp Leu Gln Val Lys Leu Ile Pro Ile Thr Ser Gln 85 90 95 Asn
Arg Ile Pro Leu Leu Gln Asn Gly Thr Phe Asp Phe Glu Cys Gly 100 105
110 Ser Thr Thr Asn Asn Val Glu Arg Gln Lys Gln Ala Ala Phe Ser Asp
115 120 125 Thr Ile Phe Val Val Gly Thr Arg Leu Leu Thr Lys Lys Gly
Gly Asp 130 135 140
Ile Lys Asp Phe Ala Asn Leu Lys Asp Lys Ala Val Val Val Thr Ser 145
150 155 160 Gly Thr Thr Ser Glu Val Leu Leu Asn Lys Leu Asn Glu Glu
Gln Lys 165 170 175 Met Asn Met Arg Ile Ile Ser Ala Lys Asp His Gly
Asp Ser Phe Arg 180 185 190 Thr Leu Glu Ser Gly Arg Ala Val Ala Phe
Met Met Asp Asp Ala Leu 195 200 205 Leu Ala Gly Glu Arg Ala Lys Ala
Lys Lys Pro Asp Asn Trp Glu Ile 210 215 220 Val Gly Lys Pro Gln Ser
Gln Glu Ala Tyr Gly Cys Met Leu Arg Lys 225 230 235 240 Asp Asp Pro
Gln Phe Lys Lys Leu Met Asp Asp Thr Ile Ala Gln Val 245 250 255 Gln
Thr Ser Gly Glu Ala Glu Lys Trp Phe Asp Lys Trp Phe Lys Asn 260 265
270 Pro Ile Pro Pro Lys Asn Leu Asn Met Asn Phe Glu Leu Ser Asp Glu
275 280 285 Met Lys Ala Leu Phe Lys Glu Pro Asn Asp Lys Ala Leu Asn
290 295 300 <210> SEQ ID NO 29 <400> SEQUENCE: 29 000
<210> SEQ ID NO 30 <211> LENGTH: 6 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: linker sequence <400>
SEQUENCE: 30 Gly Asn Asn Ser Ala Gly 1 5 <210> SEQ ID NO 31
<211> LENGTH: 6 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: linker sequence <400> SEQUENCE: 31 Gly Ser Ala
Asp Asp Gly 1 5 <210> SEQ ID NO 32 <211> LENGTH: 6
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: linker sequence
<400> SEQUENCE: 32 Gly Gly Thr Gly Gly Ala 1 5 <210>
SEQ ID NO 33 <211> LENGTH: 18 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: linker sequence <400>
SEQUENCE: 33 ggtggtaccg gaggcgcc 18 <210> SEQ ID NO 34
<211> LENGTH: 15 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 34 agcgctggta tggtg 15 <210> SEQ ID NO
35 <211> LENGTH: 15 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 35 actctcggta gcgct 15 <210> SEQ ID NO
36 <211> LENGTH: 4 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 36 Ala Gly Met Val 1 <210> SEQ ID NO 37
<211> LENGTH: 4 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB N58V-ECFP232-Q59N
<400> SEQUENCE: 37 Thr Leu Gly Ser 1 <210> SEQ ID NO 38
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB A216-cpVenus-K217
<400> SEQUENCE: 38 aaagcgggca acaacagcgc tggtgacggc 30
<210> SEQ ID NO 39 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from pRSETB
A216-cpVenus-K217 <400> SEQUENCE: 39 atcgagggta gcgctgacga
cggaaagaaa 30 <210> SEQ ID NO 40 <211> LENGTH: 10
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: partial
sequence from pRSETB A216-cpVenus-K217 <400> SEQUENCE: 40 Lys
Ala Gly Asn Asn Ser Ala Gly Asn Gly 1 5 10 <210> SEQ ID NO 41
<211> LENGTH: 10 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from pRSETB A216-cpVenus-K217
<400> SEQUENCE: 41 Ile Glu Gly Ser Ala Asp Asp Gly Lys Lys 1
5 10 <210> SEQ ID NO 42 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from
FLII275 Pglu plasmid <400> SEQUENCE: 42 actctcggca tggacgagct
gtacaagggt ggtaccggag gcgcc 45 <210> SEQ ID NO 43 <211>
LENGTH: 33 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
partial sequence from FLII2 Pglu plasmid <400> SEQUENCE: 43
actctcggca tggacgagct gtacggtagc cga 33 <210> SEQ ID NO 44
<211> LENGTH: 51 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 44 gaattcagca agaaaggcgc cggtaccggt ggaatggtga gcaagggcga
g 51 <210> SEQ ID NO 45 <211> LENGTH: 39 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from FLII2
Pglu plasmid <400> SEQUENCE: 45 gaattcagca agaaaggtac
catggtgagc aagggcgag 39 <210> SEQ ID NO 46 <211>
LENGTH: 33 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
partial sequence from FLII2 Pglu plasmid
<400> SEQUENCE: 46 gaattcagca agaaaatggt gagcaagggc gag 33
<210> SEQ ID NO 47 <211> LENGTH: 48 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 47 ttcagcaaga aaggcgccgg taccggtgga
atggtgagca agggcgag 48 <210> SEQ ID NO 48 <211> LENGTH:
45 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: partial
sequence from FLII2 Pglu plasmid <400> SEQUENCE: 48
agcaagaaag gcgccggtac cggtggaatg gtgagcaagg gcgag 45 <210>
SEQ ID NO 49 <211> LENGTH: 42 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 49 aagaaaggcg ccggtaccgg tggaatggtg
agcaagggcg ag 42 <210> SEQ ID NO 50 <211> LENGTH: 39
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: partial
sequence from FLII2 Pglu plasmid <400> SEQUENCE: 50
aaaggcgccg gtaccggtgg aatggtgagc aagggcgag 39 <210> SEQ ID NO
51 <211> LENGTH: 36 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: partial sequence from FLII2 Pglu plasmid
<400> SEQUENCE: 51 ggcgccggta ccggtggaat ggtgagcaag ggcgag 36
<210> SEQ ID NO 52 <211> LENGTH: 33 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: partial sequence from FLII2 Pglu
plasmid <400> SEQUENCE: 52 gccggtaccg gtggaatggt gagcaagggc
gag 33 <210> SEQ ID NO 53 <211> LENGTH: 30 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: partial sequence from FLII2
Pglu plasmid <400> SEQUENCE: 53 ggtaccggtg gaatggtgag
caagggcgag 30 <210> SEQ ID NO 54 <211> LENGTH: 27
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: partial
sequence from FLII2 Pglu plasmid <400> SEQUENCE: 54
accggtggaa tggtgagcaa gggcgag 27 <210> SEQ ID NO 55
<211> LENGTH: 24 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 55 ggtggaatgg tgagcaaggg cgag 24 <210> SEQ ID NO 56
<211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 56 ggaatggtga gcaagggcga g 21 <210> SEQ ID NO 57
<211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 57 atggtgagca agggcgag 18 <210> SEQ ID NO 58
<211> LENGTH: 15 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 58 gtgagcaagg gcgag 15 <210> SEQ ID NO 59
<211> LENGTH: 12 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: partial sequence from FLII2 Pglu plasmid <400>
SEQUENCE: 59 agcaagggcg ag 12
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