U.S. patent application number 14/261615 was filed with the patent office on 2014-08-21 for method for producing peptide.
This patent application is currently assigned to Glytech, Inc.. The applicant listed for this patent is Glytech, Inc.. Invention is credited to Hiroaki Asai, Kazuhiro Fukae, Yasuhiro Kajihara, Yuri Nambu, Izumi Sakamoto.
Application Number | 20140235822 14/261615 |
Document ID | / |
Family ID | 40304384 |
Filed Date | 2014-08-21 |
United States Patent
Application |
20140235822 |
Kind Code |
A1 |
Kajihara; Yasuhiro ; et
al. |
August 21, 2014 |
METHOD FOR PRODUCING PEPTIDE
Abstract
The present invention provides a method for producing a peptide,
characterized in that it comprises converting an --SH group of a
peptide comprising an amino acid residue having the --SH group to
an --OH group, wherein said method comprises the following steps
(a) to (c): (a) allowing an --SH group in a peptide to react with a
methylating agent to convert the --SH group to an -SMe group; (b)
allowing the -SMe group obtained in the step (a) to react with a
cyanizing agent to produce a reaction intermediate; and (c)
converting the reaction intermediate obtained in the step (b) to a
peptide comprising an amino acid residue having an --OH group under
more basic conditions than the conditions in the step (b).
Inventors: |
Kajihara; Yasuhiro; (Osaka,
JP) ; Sakamoto; Izumi; (Tokushima-shi, JP) ;
Nambu; Yuri; (Tokushima-shi, JP) ; Fukae;
Kazuhiro; (Tokushima-shi, JP) ; Asai; Hiroaki;
(Tokushima-shi, JP) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Glytech, Inc. |
Kyoto-shi |
|
JP |
|
|
Assignee: |
Glytech, Inc.
Kyoto-shi
JP
|
Family ID: |
40304384 |
Appl. No.: |
14/261615 |
Filed: |
April 25, 2014 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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12671238 |
Mar 7, 2011 |
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PCT/JP2008/063659 |
Jul 30, 2008 |
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14261615 |
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Current U.S.
Class: |
530/322 ;
530/327; 530/328; 530/330 |
Current CPC
Class: |
C07K 14/001 20130101;
C07K 1/1133 20130101; C07K 1/04 20130101; C07K 5/1008 20130101;
C07K 14/4727 20130101; C07K 1/1077 20130101; C07K 9/00 20130101;
C07K 7/06 20130101 |
Class at
Publication: |
530/322 ;
530/330; 530/328; 530/327 |
International
Class: |
C07K 1/113 20060101
C07K001/113; C07K 9/00 20060101 C07K009/00 |
Foreign Application Data
Date |
Code |
Application Number |
Jul 31, 2007 |
JP |
2007-199372 |
Claims
1. A method for producing a peptide comprising an amino acid
residue having an --OH group, comprising converting an --SH group
of a peptide comprising an amino acid residue having the --SH group
to the --OH group, wherein said method comprises the following
steps (a) to (c): (a) allowing the --SH group in a peptide to react
with a methylating agent to convert the --SH group to an --SMe
group; (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent; and (c) modifying the reaction
conditions to become more basic than the conditions in the step
(b); wherein the amino acid residue having the --SH group is a
threonine derivative A residue represented by the following Formula
(1), ##STR00017## the --SH group of the threonine derivative A
residue in the peptide reacts with the methylating agent in the
step (a), and the threonine derivative A residue is converted to a
threonine residue by converting the --SH group to the --OH
group.
2. The method for producing a peptide according to claim 1, wherein
the production method further comprises the following step (o)
before the step (a), and the --SH group of the threonine derivative
A residue represented by the following Formula (1) in the peptide
obtained in the step (o) reacts with a methylating agent in the
step (a): (o) ligating a first peptide containing, at the
C-terminus thereof, an amino acid residue in which a carboxyl group
is substituted with a thioester group represented by the formula
--C(.dbd.O)--SR (wherein R is selected from a benzyl group, an aryl
group, and an alkyl group, which may be substituted with
substituents), to a second peptide containing, at the N-terminus
thereof, a threonine derivative A residue according to a ligation
method to obtain a peptide comprising a threonine derivative A
represented by the following Formula (1) ##STR00018## as an amino
acid residue.
3. A method for producing a peptide comprising an amino acid
residue having an --OH group, comprising converting an --SH group
of a peptide comprising an amino acid residue having the --SH group
to the --OH group, wherein said method comprises the following
steps (a) to (c): (a) allowing the --SH group in a peptide to react
with a methylating agent to convert the --SH group to an --SMe
group; (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent; and (c) modifying the reaction
conditions to become more basic than the conditions in the step
(b); wherein the peptide comprising an amino acid residue having
the --SH group is a glycopeptide having an O-linked sugar chain,
and a glycopeptide comprising an amino acid residue having the --OH
group is obtained.
4. A method for producing a peptide comprising an amino acid
residue having an --OH group, comprising converting an --SH group
of a peptide comprising an amino acid residue having the --SH group
to the --OH group, wherein said method comprises the following
steps (a) to (c): (a) allowing the --SH group in a peptide to react
with a methylating agent to convert the --SH group to an --SMe
group; (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent; and (c) modifying the reaction
conditions to become more basic than the conditions in the step
(b); wherein the peptide comprising an amino acid residue having
the --SH group is a glycopeptide, and a glycopeptide comprising an
amino acid residue having the --OH group is obtained; and wherein
the amino acid residue having the --SH group is a threonine
derivative A represented by the following Formula (1), and the
production method further comprises the following step (o) before
the step (a), and the --SH group of the threonine derivative A
residue in the glycopeptide obtained in the step (o) reacts with
the methylating agent in the step (a): (o) ligating a first
glycopeptide whose C-terminus is represented by the following
formula: -sugar Asn-X--C(.dbd.O)--SR (wherein sugar Asn represents
a sugar chain-added asparagine, X represents a portion other than a
carboxyl group of any given amino acid residue other than proline,
and R is selected from a benzyl group, an aryl group, and an alkyl
group, which may be substituted with substituents), to a second
peptide containing a threonine derivative A residue at the
N-terminus thereof according to a ligation method to obtain a
glycopeptide containing a threonine derivative A represented by the
following Formula (1): ##STR00019## as an amino acid residue.
5. A glycopeptide having a structure represented by the following
formula: -sugar Asn-X--Y-- wherein sugar Asn represents a sugar
chain-added asparagine, X represents any given amino acid residue
other than proline, and Y represents a threonine derivative A
residue represented by Formula (2): ##STR00020##
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a division of U.S. patent application
Ser. No. 12/671,238, which is the U.S. national phase of
International Application No. PCT/JP2008/0063659, filed Jul. 30,
2008, which claims priority to Japanese Patent Application No. JP
2007-199372, filed Jul. 31, 2007, each of which is incorporated
herein by reference in its entirety.
TECHNICAL FIELD
[0002] The present invention relates to a method for producing a
peptide and a glycopeptide.
BACKGROUND
[0003] As a method for producing a peptide, a ligation method is
useful. Among such ligation methods, a native chemical ligation
method (NCL method) is a method capable of producing a peptide
having a natural amide bond (peptide bond) at a ligation site. Such
NCL method may be applied to two unprotected peptide chains. This
method has been known as a method useful for forming a natural
amide bond at a ligation site (for example, Patent Document 1). As
shown in the FIGURE below, the NCL method involves a chemical
selective reaction between a first peptide having an
.alpha.-carboxythioester portion at the C-terminus thereof and a
second peptide having a cysteine residue at the N-terminus thereof.
In this reaction, a thiol group (SH group, which may also be
referred to as a sulfhydryl group) on the side chain of cysteine
selectively reacts with a carbonyl carbon of a thioester group, and
as a result of a thiol exchange reaction, a thioester-bound initial
intermediate is generated. This intermediate intramolecularly
rearranges on a voluntary basis to give a natural amide bond to a
ligation site. At the same time, the intermediate regenerates thiol
on the side chain of cysteine. Using this reaction, it became
possible to efficiently synthesize various polypeptides.
##STR00001##
Native Chemical Ligation Method
[0004] The main disadvantage of a typical NCL method is that either
one of two peptide fragments to be ligated must have a cysteine
residue at the N-terminus thereof, and that a peptide obtained
after ligation also must have a cysteine residue at the ligation
site in this method. Accordingly, in a case in which a desired
peptide to be synthesized does not contain a cysteine residue, this
method cannot be applied.
[0005] In addition, in a typical NCL method, two or more peptide
fragments to be ligated are prepared by a solid-phase synthesis
method, for example. When a peptide contains an extremely small
amount of cysteine (or contains no cysteine), like a peptide
existing in a living body, it has been necessary to prepare an
extremely long peptide fragment to be subjected to such NCL method.
Thus, it cannot be said that this is an efficient method.
[0006] On the other hand, it has been known that various
glycopeptides and glycoproteins are present in a living body. The
sugar chains of such glycopeptides or glycoproteins are broadly
classified into two types; namely, N-linked sugar chains and
O-linked sugar chains. N-linked sugar chain is generally a sugar
chain binding to the nitrogen of amide on an asparagine side chain
via an N-glycoside bond. In general, such N-linked sugar chain
often binds to Asn in a consensus sequence -Asn-X-Ser/Thr- (wherein
X represents an amino acid other than proline) in a natural state.
O-linked sugar chain is a sugar chain binding to a hydroxyl group
on a serine or threonine side chain via an O-glycoside bond.
Examples of such N-linked and O-linked sugar chains will be given
below (Gal: galactose; GlcNAc: N-acetylglucosamine; Man: mannose;
Fuc: fucose; GalNAc: N-acetylgalactosamine). A natural glycopeptide
having such O-linked sugar chain has been known to contain large
amounts of proline, threonine, and serine (Non-Patent Documents 1
and 2).
##STR00002##
Examples of N-Linked Sugar Chain and O-Linked Sugar Chain
[0007] Patent Document 1: International Publication WO96/34878
[0008] Non-Patent Document 1: TRENDS in biochemical sciences, Vol.
27, No. 3, March 2002 [0009] Non-Patent Document 2: Cancer Biology
& Therapy 6: 4, 481-486, April 2007
DISCLOSURE OF THE INVENTION
Technical Problem
[0010] It is an object of the present invention to provide a novel
method for producing a peptide and a glycopeptide.
[0011] In particular, in a conventionally typical NCL method,
either one of two peptide fragments to be ligated must have a
cysteine residue at the N-terminus thereof, and further, a peptide
obtained after ligation also must have a cysteine residue at the
ligation site. Thus, the NCL method must have been designed and
applied by using a cysteine residue of a desired peptide (or
glycopeptide) to be finally obtained as a ligation site. Hence, the
present invention provides a novel method for producing a peptide
and a glycopeptide, which is able to design a ligation method, in
which not only a cysteine residue in a desired peptide to be
obtained, but also a portion corresponding to a serine residue or a
threonine residue can be used as a ligation site.
[0012] More specifically, in one aspect of the present invention, a
cysteine residue in a peptide (or a glycopeptide) can be converted
to a serine residue. Thus, a peptide having a cysteine residue at
the N-terminus thereof is ligated to another peptide according to
the NCL method, and thereafter, this cysteine residue can be
converted to a serine residue. Therefore, according to the present
invention, even if a cysteine residue does not exist in a desired
sequence to be obtained, if a serine residue exists therein, the
position of the serine residue can be designed as a ligation site
in the NCL method.
[0013] Moreover, in one aspect of the present invention, a peptide
having, at the N-terminus thereof, a threonine derivative residue
having an --SH group at the N-terminus thereof (or a threonine
derivative residue having an --SH group that is protected by a
disulfide bond or the like) is ligated to another peptide according
to a ligation method, and thereafter, the obtained threonine
derivative residue can be converted to a threonine residue.
Therefore, according to the present invention, even if a cysteine
residue does not exist in a desired sequence to be obtained, if a
threonine residue exists therein, the position of the threonine
residue can be designed as a ligation site in the ligation
method.
[0014] Thus, the present invention provides a novel method for
producing a peptide and a glycopeptide using a ligation method,
wherein serine or threonine that is abundant in glycopeptides can
be designed as a ligation site in the ligation method.
Solution to Problem
[0015] In order to solve the aforementioned problems, the present
invention may have the following characteristics.
[0016] The present invention may provide a method for producing a
peptide, characterized in that it comprises converting an --SH
group of a peptide comprising an amino acid residue having the --SH
group to an --OH group, wherein said method comprises the following
steps (a) to (c):
[0017] (a) allowing an --SH group in a peptide to react with a
methylating agent;
[0018] (b) allowing an --SMe group obtained in the step (a) to
react with a cyanizing agent; and
[0019] (c) modifying the reaction conditions to become more basic
than the conditions in the step (b).
[0020] The present invention may also provide a method for
producing a peptide, characterized in that it comprises converting
an --SH group of a peptide comprising an amino acid residue having
the --SH group to an --OH group, wherein said method comprises the
following steps (a) to (c):
[0021] (a) allowing an --SH group in a peptide to react with a
methylating agent to convert the --SH group to an --SMe group;
[0022] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0023] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising an amino acid residue having an
--OH group under more basic conditions than the conditions in the
step (b).
[0024] The present invention may also provide a method for
producing a peptide, characterized in that it comprises converting
a cysteine residue of a peptide comprising the cysteine residue to
a serine residue, wherein said method comprises the following steps
(a) to (c):
[0025] (a) allowing an --SH group of a cysteine residue in a
peptide to react with a methylating agent to convert the --SH group
to an --SMe group;
[0026] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0027] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising a serine residue under more basic
conditions than the conditions in the step (b).
[0028] The present invention may also provide a method for
producing a peptide, characterized in that it comprises converting
a threonine derivative A residue represented by the following
Formula (1) of a peptide comprising the threonine derivative A
residue as an amino acid residue to a threonine residue,
##STR00003##
[0029] wherein said method comprises the following steps (a) to
(c):
[0030] (a) allowing an --SH group of a threonine derivative A
residue in a peptide to react with a methylating agent to convert
the --SH group to an --SMe group;
[0031] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0032] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising a threonine residue under more
basic conditions than the conditions in the step (b).
[0033] The present invention may also provide a method for
producing a peptide, characterized in that it comprises converting
an --SMe group of a peptide comprising an amino acid residue having
the --SMe group to an --OH group, wherein said method comprises the
following steps (b) and (c):
[0034] (b) allowing an --SMe group in a peptide to react with a
cyanizing agent to produce a reaction intermediate; and
[0035] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising an amino acid residue having an
--OH group under more basic conditions than the conditions in the
step (b).
[0036] The present invention may also provide a method for
producing a peptide comprising an amino acid residue having an --OH
group, which comprises the following steps:
[0037] (o) ligating a first peptide containing, at the C-terminus
thereof, an amino acid residue in which a carboxyl group is
substituted with an .alpha.-carboxythioester group represented by
the formula --C(.dbd.O)--SR (wherein R is selected from a benzyl
group, an aryl group, and an alkyl group, which may be substituted
with substituents), to a second peptide containing, at the
N-terminus thereof, an amino acid residue having an --SH group
according to a ligation method to obtain a peptide comprising an
amino acid residue having an --SH group;
[0038] (a) allowing the --SH group in the peptide obtained in the
step (o) to react with a methylating agent to convert the --SH
group to an --SMe group;
[0039] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0040] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising an amino acid residue having an
--OH group under more basic conditions than the conditions in the
step (b).
[0041] The present invention may also provide a method for
producing a peptide comprising a serine residue, which comprises
the following steps:
[0042] (o) ligating a first peptide containing, at the C-terminus
thereof, an amino acid residue in which a carboxyl group is
substituted with an .alpha.-carboxythioester group represented by
the formula --C(.dbd.O)--SR (wherein R is selected from a benzyl
group, an aryl group, and an alkyl group, which may be substituted
with substituents), to a second peptide containing, at the
N-terminus thereof, a cysteine residue according to a ligation
method to obtain a peptide comprising a cysteine residue;
[0043] (a) allowing an --SH group of the cysteine residue in the
peptide obtained in the step (o) to react with a methylating agent
to convert the --SH group to an --SMe group;
[0044] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0045] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising a serine residue under more basic
conditions than the conditions in the step (b).
[0046] The present invention may also provide a method for
producing a peptide comprising a threonine residue, which comprises
the following steps:
[0047] (o) ligating a first peptide containing, at the C-terminus
thereof, an amino acid residue in which a carboxyl group is
substituted with an .alpha.-carboxythioester group represented by
the formula --C(.dbd.O)--SR (wherein R is selected from a benzyl
group, an aryl group, and an alkyl group, which may be substituted
with substituents), to a second peptide containing, at the
N-terminus thereof, a threonine derivative residue according to a
ligation method to obtain a peptide comprising a threonine
derivative A represented by the aforementioned Formula (1) as an
amino acid residue;
[0048] (a) allowing an --SH group of the threonine derivative A
residue in the peptide obtained in the step (o) to react with a
methylating agent to convert the --SH group to an --SMe group;
[0049] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0050] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising a threonine residue under more
basic conditions than the conditions in the step (b).
[0051] The present invention may also provide a method for
producing a glycopeptide, characterized in that it comprises
converting an --SH group of a glycopeptide comprising an amino acid
residue having the --SH group to an --OH group, wherein said method
comprises the following steps (a) to (c):
[0052] (a) allowing an --SH group in a glycopeptide to react with a
methylating agent;
[0053] (b) allowing an --SMe group obtained in the step (a) to
react with a cyanizing agent; and
[0054] (c) modifying the reaction conditions to become more basic
than the conditions in the step (b).
[0055] The present invention may also provide a method for
producing a glycopeptide, characterized in that it comprises
converting an --SH group of a glycopeptide comprising an amino acid
residue having the --SH group to an --OH group, wherein said method
comprises the following steps (a) to (c):
[0056] (a) allowing an --SH group in a glycopeptide to react with a
methylating agent to convert the --SH group to an --SMe group;
[0057] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0058] (c) converting the reaction intermediate obtained in the
step (b) to a glycopeptide comprising an amino acid residue having
an --OH group under more basic conditions than the conditions in
the step (b).
[0059] The present invention may also provide a method for
producing a glycopeptide, characterized in that it comprises
converting a cysteine residue of a glycopeptide comprising the
cysteine residue to a serine residue, wherein said method comprises
the following steps (a) to (c):
[0060] (a) allowing an --SH group of a cysteine residue in a
glycopeptide to react with a methylating agent to convert the --SH
group to an --SMe group;
[0061] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0062] (c) converting the reaction intermediate obtained in the
step (b) to a glycopeptide comprising a serine residue under more
basic conditions than the conditions in the step (b).
[0063] The present invention may also provide a method for
producing a glycopeptide, characterized in that it comprises
converting a threonine derivative A residue represented by the
aforementioned Formula (1) of a glycopeptide comprising the
threonine derivative A residue as an amino acid residue to a
threonine residue, wherein said method comprises the following
steps (a) to (c):
[0064] (a) allowing an --SH group of a threonine derivative A
residue in a glycopeptide to react with a methylating agent to
convert the --SH group to an --SMe group;
[0065] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0066] (c) converting the reaction intermediate obtained in the
step (b) to a glycopeptide comprising a threonine residue under
more basic conditions than the conditions in the step (b).
[0067] The present invention may also provide a method for
producing a glycopeptide comprising an amino acid residue having an
--OH group, which comprises the following steps:
[0068] (o) ligating a first peptide or glycopeptide containing, at
the C-terminus thereof, an amino acid residue in which a carboxyl
group is substituted with an .alpha.-carboxythioester group
represented by the formula --C(.dbd.O)--SR (wherein R is selected
from a benzyl group, an aryl group, and an alkyl group, which may
be substituted with substituents), to a second peptide or
glycopeptide containing, at the N-terminus thereof, an amino acid
residue having an --SH group according to a ligation method,
provided that at least one of the first peptide or glycopeptide and
a second peptide or glycopeptide is a glycopeptide to obtain a
glycopeptide comprising an amino acid residue having an --SH
group;
[0069] (a) allowing the --SH group in the glycopeptide obtained in
the step (o) to react with a methylating agent to convert the --SH
group to an --SMe group;
[0070] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0071] (c) converting the reaction intermediate obtained in the
step (b) to a glycopeptide comprising an amino acid residue having
an --OH group under more basic conditions than the conditions in
the step (b).
[0072] The present invention may also provide a method for
producing a glycopeptide comprising a serine residue, which
comprises the following steps:
[0073] (o) ligating a first glycopeptide whose C-terminus is
represented by the following formula:
-sugar Asn-X--C(.dbd.O)--SR
[0074] (wherein sugar Asn represents a sugar chain-added
asparagine,
[0075] X represents a portion other than a carboxyl group of any
given amino acid residue other than proline, and
[0076] R is selected from a benzyl group, an aryl group, and an
alkyl group, which may be substituted with substituents), to a
second peptide containing a cysteine residue at the N-terminus
thereof according to a ligation method to obtain a glycopeptide
containing a cysteine residue;
[0077] (a) allowing an --SH group of the cysteine residue in the
glycopeptide obtained in the step (o) to react with a methylating
agent to convert the --SH group to an --SMe group;
[0078] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0079] (c) converting the reaction intermediate obtained in the
step (b) to a glycopeptide comprising a serine residue under more
basic conditions than the conditions in the step (b).
[0080] The present invention may also provide a method for
producing a glycopeptide comprising a threonine residue, which
comprises the following steps:
[0081] (o) ligating a first glycopeptide whose C-terminus is
represented by the following formula:
-sugar Asn-X--C(.dbd.O)--SR
[0082] (wherein sugar Asn represents a sugar chain-added
asparagine,
[0083] X represents a portion other than a carboxyl group of any
given amino acid residue other than proline, and
[0084] R is selected from a benzyl group, an aryl group, and an
alkyl group, which may be substituted with substituents), to a
second peptide containing a threonine derivative residue at the
N-terminus thereof according to a ligation method to obtain a
glycopeptide containing a threonine derivative A represented by the
aforementioned Formula (1) as an amino acid residue;
[0085] (a) allowing an --SH group of the threonine derivative A
residue in the glycopeptide obtained in the step (o) to react with
a methylating agent to convert the --SH group to an --SMe
group;
[0086] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0087] (c) converting the reaction intermediate obtained in the
step (b) to a glycopeptide comprising a threonine residue under
more basic conditions than the conditions in the step (b).
[0088] The present invention may also provide a glycopeptide having
a structure represented by the following formula:
-sugar Asn-X--Y--
[0089] wherein sugar Asn represents a sugar chain-added
asparagine,
[0090] X represents any given amino acid residue other than
proline, and
[0091] Y represents a threonine derivative A residue represented by
Formula (2):
##STR00004##
[0092] In one embodiment of the present invention, the methionine
residue in the peptide or glycopeptide in the step (a) or step (o)
may preferably be a protected methionine residue, and in which the
production method preferably further comprises the following step
(d), after the step (b) or (c), and particularly after the step
(c), as desired:
[0093] (d) deprotecting the protected methionine residue.
[0094] In one embodiment of the present invention, the reaction
intermediate obtained in the step (b) may preferably be an ester
form.
[0095] In one embodiment of the present invention, the step (b) may
preferably be carried out under acidic conditions, and particularly
at pH 2 to 3.
[0096] In one embodiment of the present invention, the cyanizing
agent used in the step (b) is preferably cyanogen bromide.
[0097] In one embodiment of the present invention, the step (c) may
preferably be carried out under weakly basic conditions, for
example, at pH 7 to 9, and particularly at pH 7 to 8. When the step
(c) is carried out under weakly basic conditions, in one
embodiment, the step (c) may preferably be carried out for
approximately 10 minutes or more, and particularly for
approximately 15 minutes or more (for example, for approximately 10
minutes to 30 hours, and particularly for approximately 15 minutes
to 30 hours).
[0098] In one embodiment of the present invention, the step (c) may
preferably be carried out under strongly basic conditions, for
example, at pH 9 to 13, and particularly at pH 10 to 11. When the
step (c) is carried out under strongly basic conditions, in one
embodiment, the step (c) may preferably be carried out for
approximately 1 hour or less, and particularly for approximately 10
minutes or less (for example, for approximately 5 minutes to 1
hour, and particularly for approximately 5 minutes to 10
minutes).
[0099] In one embodiment of the present invention, when a threonine
derivative residue is contained at the N-terminus of the second
peptide, the aforementioned threonine derivative residue may be an
N-terminal amino acid residue of a threonine derivative represented
by the following Formula (3):
##STR00005##
[0100] (wherein R represents H or a protecting group for a thiol
group that is easily deprotected under conditions of a ligation
reaction, and such R preferably represents H or a disulfide
group).
[0101] In one embodiment of the present invention, either one of,
or more specifically both of, the first peptide (or glycopeptide)
and the second peptide (or glycopeptide) preferably may not contain
cysteine, or contain protected cysteine.
[0102] In one embodiment of the present invention, either the first
peptide (or glycopeptide) or the second peptide (or glycopeptide)
may preferably be a peptide (or a glycopeptide) having 80 or less,
preferably 50 or less, and more preferably 30 or less amino acid
residues.
[0103] In one embodiment of the present invention, a sugar chain in
a glycopeptide may preferably be an N-linked sugar chain or an
O-linked sugar chain.
[0104] In one embodiment of the present invention, the sugar chain
represented by the following Formula (4) may be preferable as a
sugar chain.
##STR00006##
[0105] [wherein each of R.sup.1 and R.sup.2 independently
represents a hydrogen atom or a group represented by each of
Formulas (5) to (8)].
##STR00007##
[0106] In one embodiment of the present invention, the production
method may preferably further comprise a step of adding a sugar
chain after the step (c) or the step (d).
[0107] In one embodiment, all amide bonds in the peptide or
glycopeptide obtained by the production method of the present
invention may preferably be natural amide bonds.
[0108] In one embodiment, all constitutive amino acids of the
peptide or glycopeptide obtained by the production method of the
present invention may preferably be amino acids existing as
constitutive amino acids of a peptide or glycopeptide in a living
body.
Advantageous Effects of Invention
[0109] According to the method for producing a peptide of the
present invention, an --SH group of a peptide having such --SH
group can be converted to an --OH group. In addition, an --SH group
of a peptide comprising an amino acid residue having such --SH
group, which is obtained by ligating a first peptide having an
.alpha.-carboxythioester portion represented by --C(.dbd.O)--SR at
the C-terminus thereof to a second peptide comprising an amino acid
residue having an --SH group at the N-terminus thereof according to
a ligation method, can be converted to an --OH group. These methods
can also be applied to glycopeptides.
[0110] Thus, according to the method for producing a peptide of the
present invention, a cysteine residue in a peptide can be converted
to a serine residue. As a result, even if a cysteine residue does
not exist in a desired sequence to be obtained, if a serine residue
exists therein, the NCL method can be applied.
[0111] Moreover, the present invention also provides a ligation
method in which a threonine derivative having an --SH group is used
as a ligation site. Since a threonine derivative residue having an
--SH group in a peptide obtained by this ligation method can be
converted to a threonine residue, it becomes possible to apply a
ligation method using a threonine residue as a ligation site to the
production of a peptide having threonine.
[0112] Cysteine, which has been used as a ligation site in the
conventional native chemical ligation method, is contained in a
small amount in a peptide existing in a living body. According to
the method of the present invention, serine and threonine, which
are contained in large amounts in a peptide, and particularly, in a
glycopeptide existing in a living body, can be designed as new
ligation sites in a ligation method.
[0113] Furthermore, the aforementioned method is applied to a
glycopeptide, and particularly, to a glycopeptide having an
O-linked sugar chain containing large amounts of serine and
threonine, or a glycopeptide having an N-linked sugar chain having,
as a consensus sequence, the sequence -sugar Asn-X-Ser- or -sugar
Asn-X-Thr (wherein the sugar Asn represents a sugar chain-added
asparagine, and X represents any given amino acid residue other
than proline) to produce, utilizing a ligation method, a
glycopeptide having an N-linked sugar chain or an O-linked sugar
chain, which has the same structure as that of a natural
glycopeptide.
BEST MODE FOR CARRYING OUT THE INVENTION
[0114] In a first aspect, the present invention relates to
subjecting a peptide comprising an amino acid residue having an
--SH group to the following steps (a) to (c) to obtain a peptide
comprising an amino acid residue having an --OH group:
[0115] (a) allowing an --SH group in a peptide to react with a
methylating agent;
[0116] (b) allowing an --SMe group obtained in the step (a) to
react with a cyanizing agent; and
[0117] (c) modifying the reaction conditions to become more basic
than the conditions in the step (b).
[0118] The above described steps (a) to (c) are more specifically
the following steps:
[0119] (a) allowing an --SH group in a peptide to react with a
methylating agent to convert the --SH group to an --SMe group;
[0120] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0121] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising an amino acid residue having an
--OH group under more basic conditions than the conditions in the
step (b).
[0122] In a second aspect, the present invention relates to
performing the following steps:
[0123] ligating a first peptide containing, at the C-terminus
thereof, an amino acid residue in which a carboxyl group is
substituted with an .alpha.-carboxythioester group represented by
the formula --C(.dbd.O)--SR (wherein R is selected from a benzyl
group, an aryl group, and an alkyl group, which may be substituted
with substituents), to a second peptide containing, at the
N-terminus thereof, an amino acid residue having an --SH group
according to a ligation method to obtain a peptide comprising an
amino acid residue having an --SH group; and
[0124] steps including the above described steps (a) to (c) to
obtain a peptide comprising an amino acid residue having an --OH
group.
[0125] In a third aspect, the present invention relates to
subjecting a glycopeptide comprising an amino acid residue having
an --SH group to steps including the above described steps (a) to
(c) to obtain a glycopeptide comprising an amino acid residue
having an --OH group.
[0126] In a fourth aspect, the present invention relates to
performing the following steps:
[0127] ligating a first glycopeptide whose C-terminus is
represented by the following formula:
-sugar Asn-X--C(.dbd.O)--SR
[0128] (wherein sugar Asn represents a sugar chain-added
asparagine, X represents a portion other than a carboxyl group of
any given amino acid residue other than proline, and R is selected
from a benzyl group, an aryl group, and an alkyl group, which may
be substituted with substituents), to a second peptide comprising
an amino acid residue having an --SH group at the N-terminus
thereof according to a ligation method to obtain a glycopeptide
comprising an amino acid residue having an --SH group; and
[0129] steps including the above described steps (a) to (c) to
obtain a glycopeptide comprising an amino acid residue having an
--OH group.
[0130] In the present specification, the "peptide" is not
particularly limited, as long as two or more amino acids bind to
one another via an amide bond therein. Thus, the term "peptide" is
used herein to include a known peptide, a novel peptide, and a
modified peptide form. In the present invention, those generally
referred to as proteins are also included in such peptide. In a
preferred aspect, in a peptide (or a glycopeptide) obtained by the
production method of the present invention, two or more amino acids
bind to one another via the same amide bond (peptide bond) as that
of a natural peptide or glycopeptide.
[0131] In the present specification, the term "modified peptide
form" is used to mean a compound obtained by naturally or
artificially modifying a peptide. Examples of such modification
include alkylation, acylation (for example, acetylation), amidation
(for example, amidation of the C-terminus of a peptide),
carboxylation, esterification, the formation of a disulfide bond,
glycosylation, lipidation, phosphorylation, hydroxylation, and the
binding of a labeling substance, all of which are performed on one
or multiple amino acid residues of a peptide.
[0132] In the present specification, the term "amino acid" is used
in the broadest sense. Thus, in the present specification, the term
"amino acid" includes not only natural amino acids such as serine
(Ser), asparagine (Asn), valine (Val), leucine (Leu), isoleucine
(Ile), alanine (Ala), tyrosine (Tyr), glycine (Gly), lysine (Lys),
arginine (Arg), histidine (His), aspartic acid (Asp), glutamic acid
(Glu), glutamine (Gln), threonine (Thr), cysteine (Cys), methionine
(Met), phenylalanine (Phe), tryptophan (Trp) and proline (Pro), but
also non-natural amino acids such as amino acid mutants and
derivatives. Taking into consideration such broad definitions,
persons skilled in the art could understand that the amino acid in
the present specification includes, for example: L-amino acids;
D-amino acids; chemically modified amino acids such as amino acid
mutants and derivatives; amino acids that do not become constituent
materials for proteins in a living body, such as norleucine,
.beta.-alanine, and ornithine; and chemically synthesized compounds
having amino acid properties known to persons skilled in the art.
Examples of a non-natural amino acid include .alpha.-methylamino
acids .alpha.-methylalanine, etc.), D-amino acids, histidine-like
amino acids (2-amino-histidine, .beta.-hydroxy-histidine,
homohistidine, .alpha.-fluoromethyl-histidine,
.alpha.-methyl-histidine, etc.), amino acids having excessive
methylene on their side chain ("homo" amino acids), amino acids
whose carboxylic acid functional group in the side chain is
substituted with a sulfonic acid group (cysteic acid, etc.), as
well as a threonine derivative A as described in detail below.
[0133] In the present specification, the term "threonine
derivative" is used to mean a compound represented by the following
Formula (3):
##STR00008##
[0134] In Formula (3), R represents H or a protecting group for a
thiol group that is easily deprotected under conditions of a
ligation reaction, and such R preferably represents H or a
disulfide group. In particular, a compound represented by the
following Formula (1), wherein R is H in the above Formula (3), is
referred to as a threonine derivative A.
##STR00009##
[0135] The threonine derivative of Formula (3) is a compound in
which an --OH group portion of threonine is an --SH group. Such
threonine derivative includes those having all types of
configurations. In the production method of the present invention,
it is considered that space inversion takes place when an --SH
group of an amino acid residue in a peptide is converted to an --OH
group. Thus, particularly when threonine existing in the nature is
to be obtained, a threonine derivative having an --SR group whose
configuration is inverted with respect to an --OH group of
threonine existing in the nature may preferably be used.
[0136] The aforementioned threonine derivative can be obtained by
the following method, referring to the examples and the synthesis
examples as described later, for instance.
[0137] First, threonine comprising an amino group and a carboxyl
group that have been protected is prepared. The types of such
protecting groups are not particularly limited, as long as a
peptide of interest can be obtained in the subsequent reaction. For
example, threonine with an amino group protected by a Boc group and
a carboxyl group protected by TMSE (trimethylsilylethyl) group can
be used. Thereafter, the hydroxyl group at .beta.-position is
mesylated by a known method. Subsequently, using DBU and thioacetic
acid, for example, this mesyl group is substituted into a
thioacetyl group (see D. Crich et al, J. Am. Chem. Soc., 129, 10064
(2007)).
[0138] According to a known method, this thioacetyl group is
converted to a thiol group protected by a protecting group known to
persons skilled in the art, such as a disulfide group, an
acetamidemethyl group, a nitrobenzyl group, or a trityl group. For
example, when the thioacetyl group is converted to a thiol group
protected by a disulfide group, Synthesis Example 1 as described
later can be used as a reference. The disulfide group is easily
deprotected under the reaction conditions for the subsequent
ligation method.
[0139] In a preferred aspect, the peptide (or glycopeptide)
obtained by the production method of the present invention consists
of amino acids that are all present as constitutive amino acids of
the peptide (or glycopeptide) in a living body. In addition, in one
aspect of the present invention, the peptide obtained by the
production method of the present invention is preferably a peptide
that does not contain a cysteine residue or contains small
quantities of cysteine residues in the constitutive amino acids.
Moreover, in one aspect of the present invention, the peptide
obtained by the production method of the present invention has 80
or less, preferably 50 or less, and more preferably 30 or less
amino acid residues between any given serine residue or threonine
residue and the next serine residue or threonine residue, or the
N-terminus or the C-terminus. For instance, in one aspect of the
present invention, the peptide obtained by the production method of
the present invention has one or more serine residues or threonine
residues in 5 to 40 amino acid residues, and preferably in 20 to 30
amino acid residues.
[0140] In the present specification, the term "reaction
intermediate" is used to mean all compounds produced in the period
between the reaction of an --SMe group in a peptide with a
cyanizing agent and the subsequent conversion of the --SMe group to
an --OH group, in a broad sense. The reaction scheme of the present
invention is considered to be the following scheme 1. In the scheme
1, the ester form represented by C is also a reaction intermediate
in the present invention. The present specification contains the
description "peptide-OH" in the following scheme 1, for example.
Such "--OH" means the --OH of the C-terminal carboxyl group of the
peptide, unless otherwise specified.
##STR00010## ##STR00011##
[0141] In the present specification, the term "glycopeptide" is not
particularly limited, as long as it is a compound formed by adding
at least one sugar chain to the aforementioned peptide. The
glycopeptide includes known glycopeptides and novel glycopeptides.
Those generally referred to as glycoproteins are also included in
the glycopeptide of the present invention.
[0142] In a preferred aspect, the glycopeptide obtained by the
production method of the present invention is a peptide having an
N-linked sugar chain or an O-linked sugar chain. Examples of such
glycopeptide include a part or all of peptides such as
erythropoietin, interleukin, interferon-.beta., an antibody, and a
monocyte chemotactic factor protein-3 (MCP-3).
[0143] In one aspect of the present invention, the glycopeptide
obtained by the production method of the present invention has 80
or less, preferably 50 or less, and more preferably 30 or less
amino acid residues between any given serine residue or threonine
residue to which the sugar chain is not added and the next serine
residue or threonine residue, or the N-terminus or the C-terminus
to which the sugar chain is not added. For instance, in one aspect
of the present invention, the glycopeptide obtained by the
production method of the present invention has one or more serine
residues or threonine residues in 5 to 40 amino acid residues, and
preferably in 20 to 30 amino acid residues.
[0144] In the case of such glycopeptide, a sugar chain may bind to
an amino acid residue in the peptide, directly or via a linker. The
binding site of a sugar chain and an amino acid is not particularly
limited. An amino acid preferably binds to the reducing terminus of
a sugar chain.
[0145] The type of an amino acid to which a sugar chain binds is
not particularly limited. A sugar chain may bind to either a
natural amino acid or a non-natural amino acid. From the viewpoint
that the present glycopeptide should have a structure identical to
or similar to that of a glycopeptide (glycoprotein) existing in a
living body, a sugar chain preferably binds to Asn as in the case
of an N-linked sugar chain, or to Ser or Thr as in the case of an
O-linked sugar chain. In particular, in the case of an N-linked
sugar chain, the glycopeptide obtained by the production method of
the present invention preferably has a structure (-sugar
Asn-X-Thr/Ser-) in which a sugar chain binds to Asn, an amino acid
(X) other than proline binds to the C-terminal side of the Asn via
an amide bond (peptide bond), and further, Thr or Ser binds to the
C-terminal side of the X via an amide bond (peptide bond).
[0146] When a sugar chain binds to an amino acid via a linker, from
the viewpoint of the property of easily binding to the linker,
preferred amino acids to which the sugar chain binds include: an
amino acid having two or more carboxyl groups in its molecule, such
as aspartic acid or glutamic acid; an amino acid having two or more
amino groups in its molecule, such as lysine, arginine, histidine,
or tryptophan; an amino acid having a hydroxyl group in its
molecule, such as serine, threonine, or tyrosine; an amino acid
having a thiol group in its molecule, such as cysteine; and an
amino acid having an amide group in its molecule, such as
asparagine or glutamine. In particular, from the viewpoint of
reactivity, aspartic acid, glutamic acid, lysine, arginine, serine,
threonine, cysteine, asparagine, and glutamine are preferable.
[0147] In a case in which a sugar chain binds to an amino acid via
a linker in a glycopeptide, linkers that have been widely used in
the present field can be used herein as linkers. Examples of such
linker include:
--NH--(CO)--(CH.sub.2).sub.a--CH.sub.2--
[0148] (wherein a is an integer, which is not particularly limited
unless it interferes a linker function of interest, and is
preferably an integer from 0 to 4); C.sub.1-10 polymethylene;
and
--CH.sub.2--R.sup.3--
[0149] (wherein R.sup.3 represents a group produced by dissociating
a single hydrogen atom from a group selected from the group
consisting of alkyl, substituted alkyl, alkenyl, substituted
alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, a
carbocyclic group, a substituted carbocyclic group, a heterocyclic
group, and a substituted heterocyclic group).
[0150] In the present specification, the term "sugar chain"
includes not only a compound formed by connecting two or more unit
sugars (monosaccharides and/or the derivatives thereof), but also a
compound consisting of a single unit sugar (a monosaccharide and/or
the derivative thereof). Examples of such sugar chain include a
wide range of sugar chains including: monosaccharides and
polysaccharides contained in a living body (glucose, galactose,
mannose, fucose, xylose, N-acetylglucosamine,
N-acetylgalactosamine, sialic acid, and the complex and derivative
thereof); and sugar chains decomposed or induced from complex
biomolecules such as a decomposed polysaccharide, a glycoprotein, a
proteoglycan, a glycosaminoglycan, and a glycolipid. However,
examples are not limited thereto. When two or more unit sugars are
connected with one another, a unit sugar binds to another unit
sugar due to dehydration condensation due to a glycoside bond. A
sugar chain may be of either a linear type or a branched type.
[0151] Moreover, in the present specification, sugar chain
derivatives are also included in the "sugar chain." Examples of
such sugar chain derivative include: a sugar constituting a sugar
chain, which has a carboxyl group (for example, aldonic acid whose
position C-1 has been oxidized to carboxylic acid (e.g. D-gluconic
acid obtainable by oxidizing D-glucose), and uronic acid, the C
atom at the terminus of which has been converted to carboxylic acid
(D-glucuronic acid obtainable by oxidizing D-glucose)); a sugar
having an amino group or a derivative thereof (e.g. an acetylated
amino group) (for example, N-acetyl-D-glucosamine,
N-acetyl-D-galactosamine, etc.); a sugar having both an amino group
and a carboxyl group (for example, N-acetylneuraminic acid (sialic
acid), N-acetyl muramic acid, etc.); a deoxy sugar (for example,
2-deoxy-D-ribose); a sulfated sugar containing a sulfate group; and
a phosphorylated sugar containing a phosphate group. However,
examples are not limited thereto.
[0152] The sugar chain of the present invention is preferably a
sugar chain that exists as a complex carbohydrate in a living body
(a glycopeptide (or a glycoprotein), a proteoglycan, a glycolipid,
etc.). The present sugar chain is preferably an N-linked sugar
chain, an O-linked sugar chain, or the like, which binds as a
glycopeptide (or a glycoprotein) to a peptide (or a protein) in a
living body. In an O-linked sugar chain-binding glycopeptide,
N-acetylgalactosamine (GalNAc), N-acetylglucosamine (GlcNAc),
xylose, fucose, and the like bind to the Ser or Thr of a peptide
via an O-glycoside bond, and a sugar chain is further added to such
bound body. Examples of an N-linked sugar chain include a high
mannose type, a complex type, and a hybrid type. Of these, a
complex type is preferable.
[0153] In the present invention, a preferred sugar chain is the
sugar chain represented by the following Formula (4), for
example:
##STR00012##
[0154] [wherein each of R.sup.1 and R.sup.2 independently
represents a hydrogen atom or a group represented by each of
Formulas (5) to (8)].
##STR00013##
[0155] In order to prevent problems, such as antigenicity, which
may occur when the method for producing a glycopeptide of the
present invention is applied to the field of production of
pharmaceutical products and the like, examples of a preferred sugar
chain used include: a sugar chain having a structure identical to
that of a sugar chain existing as a glycoprotein that binds to a
protein in a human body (having the same types of constituent
sugars and the same binding manners) (for example, the sugar chain
described in FEBS LETTERS Vol. 50, No. 3, February 1975); and a
sugar chain formed by losing one or multiple sugars from the
non-reducing terminus of the aforementioned sugar chain.
[0156] The number of sugar chains added to a glycopeptide is not
particularly limited, as long as it is 1 or greater. From the
viewpoint of the production of a glycopeptide having a structure
similar to that of a glycopeptide existing in a living body, the
number of sugar chains added is more preferably almost the same as
that of a glycopeptide existing in a body.
[0157] In a preferred aspect of the present invention, the
structures of sugar chains in the glycopeptides of the present
invention are uniform. In the present specification, such
expression as "the structures of sugar chains in the glycopeptides
are uniform" means that, when a comparison is made between
glycopeptides, the sites of addition of sugar chains, the types of
sugars constituting sugar chains, a binding order, and a sugar
binding manner are identical between them, and that the structures
of sugar chains are uniform at a percentage of at least 90% or
more, preferably 95% or more, and more preferably 99% or more.
Glycopeptides having uniform sugar chains are of the same quality,
and such glycopeptides are preferably used, in particular, in the
field of the production of pharmaceutical products, assay, and the
like.
[0158] A peptide used as a raw material in the production method of
the present invention can be produced by applying peptide
production methods known to persons skilled in the art, such as
solid-phase synthesis, liquid-phase synthesis, synthesis using
cells, and a method of separating and extracting
naturally-occurring products. In addition, when a glycopeptide is
used as a raw material, such glycopeptide can be produced by
incorporating a step of adding sugar chains into the aforementioned
known peptide production methods. With regard to a method for
producing sugar chains used in such sugar chain-adding step,
International Publications WO03/008431, WO2004/058984,
WO2004/058824, WO2004/070046, WO2007/011055, etc. can be
referred.
[0159] As a specific example, a method for producing a peptide or a
glycopeptide comprising an amino acid residue having an --SH group
according to a solid-phase synthesis method will be described
below. As for the below-mentioned method, International Publication
WO2004/005330 can also be referred.
[0160] First, (1) a hydroxyl group of a resin having a hydroxyl
group and a carboxyl group of an amino acid whose amino group is
protected by a fat-soluble protecting group are subjected to an
esterification reaction. In this case, since the amino group of the
amino acid is protected by the fat-soluble protecting group, the
self-condensation of amino acids is prevented, and the hydroxyl
group of the resin reacts with the carboxyl group of the amino
acid, so that esterification takes place.
[0161] Next, (2) the fat-soluble protecting group of the ester as
obtained above is dissociated to form a free amino group,
[0162] (3) this free amino group is amidated with a carboxyl group
of a desired amino acid whose amino group is protected by a
fat-soluble protecting group,
[0163] (4) the aforementioned fat-soluble protecting group is
dissociated to form a free amino group, and
[0164] (5) the aforementioned steps (3) and (4) are repeated as
necessary to obtain a peptide in which a desired number of desired
amino acids are ligated, and which has a resin at one terminus
thereof and also has a free amino group at another terminus
thereof.
[0165] Using an amino acid having an --SH group (wherein the --SH
group may be protected) (for example, cysteine or the
aforementioned threonine derivative of Formula (3)) in the
aforementioned steps (1) to (5), a peptide comprising an amino acid
residue having an --SH group can be obtained. When an --SH group of
an amino acid having the --SH group is used in the aforementioned
steps (1) to (5), the --SH group may be protected by a protecting
group known to persons skilled in the art, such as a disulfide
group, an acetamidemethyl group, a nitrobenzyl group, or a trityl
group. Thereafter, such protecting group is removed, as necessary.
Moreover, using a sugar chain-added amino acid (for example, sugar
chain asparagine formed by adding a sugar chain to asparagine,
sugar chain serine or sugar chain threonine formed by adding a
sugar chain to serine or threonine, and the like) as an amino acid
in the aforementioned steps (1) to (5), N-linked and/or O-linked
glycopeptides having one or two or more sugar chains at a desired
position(s) can also be obtained. As described above, such N-linked
and/or O-linked glycopeptides are able to comprise an amino acid
residue(s) having an --SH group(s) at a desired position(s).
[0166] (6) Thereafter, the ester bond between the resin and the
amino acid in (1) above is cleaved by an acid to produce a desired
peptide (or glycopeptide).
[0167] Generally, the type of a solid-phase resin is not
particularly limited, as long as it is a resin used in solid-phase
synthesis. For instance, Amino-PEGA resin (manufactured by Merck),
Wang resin (manufactured by Merck), HMPA-PEGA resin (manufactured
by Merck), Trt Chloride resin (manufactured by Merck), and the like
may be used.
[0168] Moreover, a linker may be disposed between such Amino-PEGA
resin and an amino acid. Examples of such linker include
4-hydroxymethylphenoxyacetic acid (HMPA) and
4-(4-hydroxymethyl-3-methoxyphenoxy)-butylacetic acid (HMPB).
[0169] Examples of a fat-soluble protecting group include:
carbonyl-containing groups such as a 9-fluorenylmethoxycarbonyl
(Fmoc) group, a t-butyloxycarbonyl (Boc) group, and an
allyloxycarbonyl (Alloc) group; protecting groups such as acyl
groups such as an acetyl (Ac) group, an allyl group and a benzyl
group. However, examples are not particularly limited thereto.
[0170] In order to introduce a fat-soluble protecting group into a
desired peptide (or glycopeptide), when an Fmoc group is introduced
for example, it can be introduced by adding
9-fluorenylmethyl-N-succinimidyl carbonate and sodium bicarbonate
to the reaction system and then carrying out a reaction. The
reaction may be carried out at a temperature between 0.degree. C.
and 50.degree. C., and preferably at a room temperature, for
approximately 1 to 5 hours.
[0171] As an amino acid protected by a fat-soluble protecting
group, the aforementioned amino acids protected by the above
described method may be used. In addition, commercially available
products may also be used. Examples of such amino acid protected by
a protecting group include Fmoc-Ser, Fmoc-Asn, Fmoc-Val, Fmoc-Leu,
Fmoc-Ile, Fmoc-Ala, Fmoc-Tyr, Fmoc-Gly, Fmoc-Lys, Fmoc-Arg,
Fmoc-His, Fmoc-Asp, Fmoc-Glu, Fmoc-Gln, Fmoc-Thr, Fmoc-Cys,
Fmoc-Met, Fmoc-Phe, Fmoc-Trp, and Fmoc-Pro.
[0172] As esterification catalysts, known dehydrating condensing
agents such as 1-mesitylenesulfonyl-3-nitro-1,2,4-triazole (MSNT),
dicyclohexylcarbodiimide (DCC), and 1,3-diisopropylcarbodiimide
(DIPCDI) may be used, for example. With regard to the usage ratio
between an amino acid and a dehydrating condensing agent, 1 to 10
parts by weight of, and preferably 2 to 5 parts by weight of the
dehydrating condensing agent is generally used with respect to 1
part by weight of the amino acid.
[0173] An esterification reaction is preferably carried out by
placing a resin in a solid-phase column, washing the resin with a
solvent, and then adding an amino acid solution thereto, for
example. Examples of such washing solvent include dimethylformamide
(DMF), 2-propanol, and methylene chloride. Examples of a solvent
for dissolving amino acid include dimethyl sulfoxide (DMSO), DMF,
and methylene chloride. Such esterification reaction may be carried
out at a temperature between 0.degree. C. and 50.degree. C., and
preferably at a room temperature, for approximately 10 minutes to
30 hours, and preferably for approximately 15 minutes to 24
hours.
[0174] During the reaction, it is also preferable that unreacted
functional groups on the solid phase be acetylated using acetic
anhydride or the like and be capped.
[0175] A fat-soluble protecting group may be dissociated by
treating it with a base, for example. Examples of such base include
piperidine and morpholine. The treatment is preferably carried out
in the presence of a solvent. Examples of a solvent used herein
include DMSO, DMF, and methanol.
[0176] The amidation reaction between a free amino group and a
carboxyl group of any given amino acid whose amino group nitrogen
is protected by a fat-soluble protecting group is preferably
carried out in the presence of an activator and a solvent.
[0177] Examples of an activator include dicyclohexylcarbodiimide
(DCC), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
(WSC/HCl), diphenylphosphoryl azide (DPPA), carbonyldiimidazole
(CU), diethylcyanophosphonate (DEPC), 1,3-diisopropylcarbodiimide
(DIPCl), benzotriazol-1-yloxy-tris(pyrrolidino)phosphonium
hexafluorophosphate (PyBOP),
3-diethoxyphosphoryloxy-1,2,3-benzotriazin-4(3H)-one (DEPBT),
1-hydroxybenzotriazole (HOBt), hydroxysuccinimide (HOSu),
dimethylaminopyridine (DMAP), 1-hydroxy-7-azabenzotriazole (HOAt),
3-hydroxy-4-oxo-3,4-dihydro-5-azabenzo-1,2,3-triazine (HODhbt),
hydroxyphthalimide (HOPht), pentafluorophenol (Pfp-OH),
2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium
hexafluorophosphate (HBTU),
O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium
hexafluorophosphonate (HATU), and
O-benzotriazol-1-yl-1,1,3,3-tetramethyluronium tetrafluoroborate
(TBTU).
[0178] With regard to the amount of an activator used, 1 to 20
equivalent amounts of, preferably 1 to 10 equivalent amounts of,
and more preferably 1 to 5 equivalent amounts of activator is used
with respect to any given amino acid whose amino group nitrogen is
protected by a fat-soluble protecting group.
[0179] The reaction proceeds only with the aforementioned
activator. However, amine is preferably added as an auxiliary
agent. As such amine, diisopropylethylamine (DIPEA),
N-ethylmorpholine (NEM), N-methylmorpholine (NMM),
N-methylimidazole (NMI), and the like can be used. With regard to
the amount of such auxiliary agent used, 1 to 20 equivalent amounts
of, preferably 1 to 10 equivalent amounts of, and more preferably 1
to 5 equivalent amounts of auxiliary agent is used with respect to
any given amino acid whose amino group nitrogen is protected by a
fat-soluble protecting group.
[0180] Examples of a solvent include DMSO, DMF, and methylene
chloride. The reaction is carried out at a temperature between
0.degree. C. and 50.degree. C., and preferably at a room
temperature, for approximately 10 to 30 hours, and preferably for
approximately 15 minutes to 24 hours. During this reaction also, it
is preferable that unreacted amino groups on the solid phase be
acetylated using acetic anhydride or the like and be capped. The
fat-soluble protecting group may be dissociated in the same manner
as that described above.
[0181] In order to cleave a peptide chain from a resin, it is
preferable to treat the resin with an acid. Examples of an acid
used herein include trifluoroacetic acid (TFA) and hydrogen
fluoride (HF). During the treatment, a highly reactive cation
species can be generated from the fat-soluble protecting group used
for amino acid and the linker on the resin. Thus, to capture such
cation species, a nucleophilic reagent is preferably added.
Examples of such nucleophilic reagent include triisopropylsilane
(TIS), phenol, thioanisole, and ethanedithiol (EDT).
[0182] Thus, a peptide (or a glycopeptide) comprising an amino acid
residue having an --SH group can be obtained.
[0183] Moreover, a method utilizing the reverse reaction of an
enzyme including transglutaminase as a typical example may be
applied to the thus obtained peptide or glycopeptide comprising an
amino acid residue having an --SH group to add a sugar chain
thereto, thereby obtaining a glycopeptide comprising an amino acid
having an --SH group.
[0184] Furthermore, it is also possible to combine a sugar chain
elongation reaction using transferase with the aforementioned
method.
[0185] Among the peptides or glycopeptides as obtained above, a
peptide (or a glycopeptide) comprising an amino acid residue having
an --SH group at the N-terminus thereof can be ligated to a peptide
(or a glycopeptide) having an .alpha.-carboxythioester portion at
the C-terminus thereof according to a ligation method.
[0186] In the present specification, the term "ligation method" is
used to include not only the native chemical ligation method (NCL
method) described in Patent Document 1, but also include the
application of such native chemical ligation method to peptides
comprising non-natural amino acids or amino acid derivatives (for
example, the threonine derivative A of Formula (1), a protected
methionine, a sugar chain-added amino acid, etc.), as described in
the after-mentioned examples. According to such ligation method, a
peptide having a natural amide bond (peptide bond) at a ligation
site can be produced.
[0187] According to the ligation method, ligation can be carried
out on all cases of between a peptide and a peptide, between a
peptide and a glycopeptide, and between a glycopeptide and a
glycopeptide.
[0188] A peptide (or a glycopeptide) having an
.alpha.-carboxythioester group at the C-terminus thereof, which is
used in the ligation method, can be produced by a method known to
persons skilled in the art, as described in Patent Document 1.
[0189] For example, as described in the after-mentioned examples, a
solid-phase synthesis method is applied to obtain a protected
peptide (or glycopeptide) whose amino groups on the amino acid side
chain and at the N-terminus are protected. A carboxyl group on the
C-terminal side thereof is condensed with benzylthiol, using
PyBOP(Benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium
hexafluorophosphate)/DIPEA as a condensing agent in a liquid phase.
Thereafter, the chain of the amino acid is deprotected using a 95%
TFA solution to obtain a peptide (or a glycopeptide) having an
.alpha.-carboxythioester group at the C-terminus thereof.
[0190] The ligation method can be carried out using a method known
to persons skilled in the art, as described in Patent Document 1,
or by referring to the descriptions of the after-mentioned
examples. For example, a first peptide having an
.alpha.-carboxythioester group represented by --C(.dbd.O)--SR at
the C-terminus thereof and a second peptide comprising an amino
acid residue having an --SH group at the N-terminus thereof are
prepared, while referring to the aforementioned descriptions. In
the first peptide, R is not particularly limited, as long as it
does not interfere a thiol exchange reaction and it acts as a
leaving group in a nucleophilic substitution reaction on a carbonyl
carbon. Preferably, such R may be selected from among benzyl-type
groups such as benzylmercaptan, aryl-type groups such as thiophenol
or 4-(carboxymethyl)-thiophenol, alkyl-type groups such as
2-mercaptoethanesulfonate or 3-mercaptopropionic acid amide, and
the like. The --SH group at the N-terminus of the second peptide
may be protected by a protecting group, as desired. This protecting
group is removed at a desired time point before the subsequent
ligation reaction, and the second peptide having an --SH group at
the N-terminus thereof reacts with the first peptide. For example,
if it is a protecting group that is spontaneously removed under
conditions in which ligation takes place, the second peptide
protected by the protecting group may be directly used in the
subsequent ligation reaction.
[0191] The two peptides are mixed with each other in a solution
such as a 100 mM phosphate buffer in the presence of catalytic
thiol such as 4-mercaptophenylacetic acid, benzylmercaptan or
thiophenol, as necessary. Preferably the reaction is carried out,
using 0.5 to 2 equivalent amounts of the second peptide and
approximately 5 equivalent amounts of the catalytic thiol with
respect to 1 equivalent amount of the first peptide. The reaction
is preferably carried out under conditions consisting of pH 6.5 to
7.5 and a temperature between 20.degree. C. and 40.degree. C. for
approximately 1 to 30 hours. The progress of the reaction can be
confirmed by a known method, in which HPLC, MS, and the like are
combined.
[0192] To the reaction product, a reducing agent such as
dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP)
hydrochloride is added to suppress side reactions, and the reaction
product is purified as desired, so that the first peptide can be
ligated to the second peptide.
[0193] In a case in which peptides having different R groups exist
among the peptides having a carboxythioester portion
(--C.dbd.O--SR) at the C-terminus thereof, the order of ligation
reactions can be altered (see Protein Science (2007), 16:
2056-2064, etc.). Thus, when ligation is carried out multiple
times, the order of ligation reactions may be taken into
consideration. For example, when an aryl group, a benzyl group, and
an alkyl group exist as R, the ligation reaction generally
progresses in this order.
[0194] In the present invention, a method for producing a peptide
(or a glycopeptide) characterized in that an --SH group of a
peptide (or a glycopeptide) comprising an amino acid residue having
the --SH group is converted to an --OH group will be specifically
described. As raw materials, the peptide or glycopeptide obtained
by the aforementioned method is used. In one aspect of the present
invention, a peptide or a glycopeptide obtained by a ligation
method is preferably used. Subsequently, the following steps (a) to
(c) are carried out:
[0195] (a) allowing an --SH group in a peptide to react with a
methylating agent to convert the --SH group to an --SMe group;
[0196] (b) allowing the --SMe group obtained in the step (a) to
react with a cyanizing agent to produce a reaction intermediate;
and
[0197] (c) converting the reaction intermediate obtained in the
step (b) to a peptide comprising an amino acid residue having an
--OH group under more basic conditions than the conditions in the
step (b). Hereafter, the case of using a peptide as a raw material
will be exemplified.
Step (a)
[0198] The methylating agent used in the methylation of the step
(a) is not particularly limited, as long as it is capable of
converting the --SH group in the peptide to the --SMe group.
Examples of such methylating agent include iodomethane and
methyl-4-nitrobenzenesulfonate.
[0199] With regard to the amount of a methylating agent used, 1 to
1,000 equivalent amounts of, preferably 10 to 100 equivalent
amounts of, and more preferably 15 to 30 equivalent amounts of the
methylating agent can be used with respect to a single residue of
the --SH group of the raw material peptide. The methylation
reaction is desirably carried out at a temperature between
0.degree. C. and 50.degree. C., and preferably between 20.degree.
C. and 30.degree. C., for approximately 10 minutes to 30 hours, and
preferably for approximately 15 minutes to 1 hour.
[0200] A buffer solution is preferably used as a solvent in the
methylation reaction. A buffer solution having pH 7 to 9, and
particularly pH 8 to 9, can be preferably used. For example, a 0.25
M Tris-HCl buffer solution (6 M guanidine hydrochloride solution,
containing 3.3 mM EDTA, pH 8.6) or the like can be used.
[0201] In a case in which a cysteine residue is contained as an
amino acid in a peptide and in which the cysteine residue is not
intended to be converted to a serine residue and it is allowed to
exist as a cysteine residue in a peptide obtained by the production
method of the present invention, such cysteine is protected and it
is introduced in the form of a protected cysteine into a peptide
according to a known method. Thereby, the --SH group of the
cysteine can be prevented from being methylated in the step (a).
Taking into consideration a thiol exchange reaction, a treatment
with acid, a treatment with base, etc. performed in each step of
the production method of the present invention, an appropriate
protecting group can be used as a protecting group for cysteine.
Examples of such protecting group include an acetamidemethyl (Acm)
group, a benzyl group, an acetamide group, and a trityl group.
Preferably, an Acm group can be used. After the steps (a) to (c),
the protected cysteine residue is deprotected using a known method.
For example, when a protected cysteine that has been protected by a
protecting group such as an Acm group, a nitrobenzyl group, or a
trityl group, is introduced, such protected cysteine is converted
to a cysteine residue by adding a step of applying a deprotection
method using a silver acetate aqueous solution, a deprotection
method using light, a deprotection method involving a treatment
with acid, or other methods. Thus, a cysteine residue is allowed to
exist in the peptide obtained by the production method of the
present invention.
[0202] In one aspect of the present invention, it is also possible
to obtain a peptide having an --OH group by converting an --SMe
group of a peptide having the SMe group to --OH group. In such a
case, the aforementioned step (a) can be omitted.
Step (b)
[0203] From the viewpoint of safety and the like, cyanogen bromide,
phenylcyanate, or the like can be used as a cyanizing agent in the
step (b), for example. Preferably, cyanogen bromide, which can be
easily procured, can be used.
[0204] With regard to the amount of a cyanizing agent used, 1 to
1,000 equivalent amounts of, preferably 10 to 100 equivalent
amounts of, and more preferably 15 to 30 equivalent amounts of the
cyanizing agent can be used with respect to a single --SMe group.
The reaction with the cyanizing agent is desirably carried out at a
temperature between 0.degree. C. and 50.degree. C., and preferably
between 30.degree. C. and 40.degree. C., for approximately 30
minutes to 100 hours, and preferably for approximately 12 hours to
50 hours.
[0205] The reaction with the cyanizing agent is carried out under
acidic conditions, and it is particularly preferably carried out at
pH 2 to 3. Using an acidic water-soluble substance, and
specifically using formic acid, trifluoroacetic acid,
methanesulfonic acid, or the like, the reaction can be carried out
under acidic conditions. During the reaction, in order to prevent
the oxidation of sulfur atoms, it is particularly preferable that
the used acidic water-soluble substance have been deaerated. In
addition, from the viewpoint of the stability of the cyanizing
agent, the reaction is preferably carried out under shading
conditions.
[0206] As a solvent, the aforementioned water-soluble solvent
having pH 2 to 3, such as a 80% formic acid solution, a 70% formic
acid solution, or a 2% trifluoroacetic acid/39%
acetonitrile-containing aqueous solution, can be preferably
used.
[0207] An example of the reaction intermediate obtained in the step
(b) is an ester form having the following structure:
##STR00014##
[0208] When a methionine residue is contained as an amino acid in
the peptide, it is preferable to distinguish the --SMe group
contained in the methionine residue from the --SMe group obtained
in the step (a). In the present specification, the protected
methionine is not particularly limited, as long as it is a compound
that does not react with the cyanizing agent in the step (b). An
example of such protected methionine is a sulfoxide-type methionine
(Met(O): --CH.sub.2--CH.sub.2--S(.dbd.O)--CH.sub.3). As described
in the after-mentioned Example 5, a protected methionine (for
example, Met(O)) is introduced into the peptide using a known
method, so that a methionine residue can be distinguished from the
--SMe group obtained in the step (a), and the methionine residue
becomes inactive on the reaction with the cyanizing agent in the
step (b). Thereafter, the protected methionine residue is converted
to a methionine residue using a known method, as appropriate (the
after-mentioned step (e)). Thus, a peptide having a methionine
residue can also be obtained by the production method of the
present invention.
[0209] Moreover, an oxidized cysteine, which is a by-product formed
during the reaction with the cyanizing agent in the step (b), can
be removed, as necessary. In such removing step, a mixture
comprising the reaction intermediate obtained in the step (b) may
be reacted at a room temperature for approximately 30 minutes in
the presence of ammonium iodide and dimethyl sulfide, for example,
and thereafter, the reaction solution may be separated and washed.
Such removing step may be carried out at any time point after the
step (b), and it may be preferably carried out after the step
(c).
Step (c)
[0210] In the step (c), under more basic conditions than those in
the step (b), a peptide comprising an amino acid residue having an
--OH group is obtained by an intramolecular acyl rearrangement from
O-- to N-- of the reaction intermediate obtained in the step
(b).
[0211] The basic conditions in the step (c) may be either acidic or
neutral, as long as they are more basic conditions than the
conditions in the step (b). More specifically, the basic conditions
in the step (c) are not particularly limited, as long as they are
conditions in which an --NH.sub.2 group on a C atom adjacent to the
ester bond of the reaction intermediate obtained in the step (b) is
not protonated. From the viewpoint of efficient conversion of the
reaction intermediate to a peptide having an --OH group, weakly
basic conditions or strongly basic conditions can be applied.
[0212] When the basic conditions in the step (c) are weakly basic
conditions, the pH is pH 7 to 9, and preferably pH 7 to 8. For
example, by adding to a solution, a basic compound that is used as
a pH adjuster known to persons skilled in the art, such as
guanidine, disodium phosphate, Tris, or sodium bicarbonate, weakly
basic conditions can be prepared. During this operation, with
regard to the amount of a basic compound used, 1 to 1,000
equivalent amounts of, preferably 10 to 100 equivalent amounts of,
and more preferably 15 to 30 equivalent amounts of such basic
compound may be used with respect to a raw material peptide.
[0213] When the basic conditions in the step (c) are weakly basic
conditions, the reaction is desirably carried out at a temperature
between 0.degree. C. and 50.degree. C., and preferably between
20.degree. C. and 40.degree. C., for approximately 10 minutes to 30
hours, and preferably for approximately 15 minutes to 30 hours. The
reaction is desirably carried out in a buffer solution having pH 7
to 9, and preferably pH 7 to 8. For example, the step (c) may be
carried out in a 0.2 M phosphate buffer (containing a 6 M guanidine
hydrochloride solution, pH 7.2).
[0214] When the basic conditions in the step (c) are weakly basic
conditions, the pH may be further decreased, and the step (c) may
be terminated. Otherwise, the routine may proceed to a purification
step using HPLC or the like, without changing the pH.
[0215] When the basic conditions in the step (c) are strongly basic
conditions, the pH is pH 9 to 13, and preferably pH 10 to 11. Such
strongly basic conditions are preferably conditions in which a
compound that has excessively reacted with a hydroxyl group can be
removed by hydrolysis. By adding to a solution, a basic
water-soluble substance such as a hydrazine hydrate, a 50 mM sodium
hydroxide aqueous solution, or the like, strongly basic conditions
can be prepared. During this operation, with regard to the amount
of a basic water-soluble substance used, 0.5 to 100 equivalent
amounts of, preferably 0.1 to 10 equivalent amounts of, and more
preferably 0.5 to 1 equivalent amounts of such basic water-soluble
substance may be used with respect to a raw material peptide. For
example, the step (c) may be carried out in a 5% hydrazine aqueous
solution having pH 10 to 11.
[0216] When the basic conditions in the step (c) are strongly basic
conditions, a peptide comprising an amino acid residue having an
--OH group can be obtained by an intramolecular acyl rearrangement
from O-- to N-- of the reaction intermediate obtained in the step
(b), and at the same time, decyanization (a decyanization reaction
for removing excessively reacted cyanizing agents by hydrolysis),
deformylation (a deformylation reaction of excessively reacted
formic acids), and the like may occur on the excessively reacted
--OH groups in the steps (a) and (b).
[0217] When the basic conditions in the step (c) are strongly basic
conditions, the step (c) is desirably carried out at a temperature
between 0.degree. C. and 50.degree. C., and preferably between
20.degree. C. and 30.degree. C., for approximately 5 minutes to 3
hours, preferably for approximately 5 minutes to 1 hour, and more
preferably for approximately 5 minutes to 10 minutes. When the
basic conditions in the step (c) are strongly basic conditions, if
the step (c) is carried out for a long period of time, side
reactions such as racemization and the cleavage of a peptide bond
may occur.
[0218] When the basic conditions in the step (c) are strongly basic
conditions, the step (c) may be terminated by decreasing the pH to
pH 4 to 9, and preferably to pH 5 to 9, for example, around pH 7,
or pH 8 to 9.
[0219] In the step (c), it is assumed that the configuration of the
position 13 of the obtained amino acid residue having an --OH group
is inverted from that of the reaction intermediate obtained in the
step (b).
Step (d)
[0220] When a peptide containing a protected methionine residue is
used as a raw material, the following step (d) is further carried
out after the step (b) or (c), as desired: (d) deprotecting the
protected methionine.
[0221] Deprotection may be carried out using a method known to
persons skilled in the art, depending on the type of the protected
methionine used. When a sulfoxide-type methionine (Met(O)) is
introduced as a protected methionine, for example, such protected
methionine is converted to methionine by adding a reduction step
using an ammonium iodide/dimethyl sulfide/TFA mixed solution or the
like. From the viewpoint of the prevention of the occurrence of
side reactions, the step (d) is preferably carried out after the
step (c).
[0222] Thus, in the case of obtaining a peptide having a methionine
residue as well, the production method of the present invention can
be applied.
[0223] For the purification of the obtained product, a method for
obtaining a 97% or more purified product under several types of
high performance liquid chromatography conditions is preferably
applied. Specific examples of such method include crystallization,
a transactional partition method, partition chromatography, a gel
filtration method, ion exchange chromatography, and high
performance liquid chromatography. Preferably, high performance
liquid chromatography or the like can be applied.
[0224] In addition to the aforementioned steps, a step of adding a
sugar chain may be further carried out. Such addition of a sugar
chain can be carried out on both a peptide and a glycopeptide. Such
addition of a sugar chain can be carried out at any time point, as
long as a glycopeptide of interest can be obtained. It is
preferably carried out after the step (c).
[0225] The addition of a sugar chain can be carried out by a method
utilizing the reverse reaction of an enzyme including
transglutaminase as a typical example, or by a method performed by
referring to the descriptions of International Publication
WO2005/010053, as described below.
[0226] First, a haloacetamide complex-type sugar chain derivative
is produced by allowing it to react with a peptide as obtained
above (in particular, a peptide comprising: an amino acid having
two or more carboxyl groups in its molecule, such as aspartic acid
or glutamic acid; an amino acid having two or more amino groups in
its molecule, such as lysine, arginine, histidine, or tryptophan;
an amino acid having a hydroxyl group in its molecule, such as
serine, threonine, or tyrosine; an amino acid having a thiol group
in its molecule, such as cysteine; or an amino acid having an amide
group in its molecule, such as asparagine or glutamine, and among
them, particularly, a peptide comprising aspartic acid, glutamic
acid, lysine, arginine, serine, threonine, cysteine, asparagine, or
glutamine). The aforementioned reaction may be carried out at a
temperature generally between 0.degree. C. and 80.degree. C.,
preferably between 10.degree. C. and 60.degree. C., and more
preferably between 15.degree. C. and 35.degree. C. Preferably, the
reaction time is generally approximately 30 minutes to 5 hours.
After completion of the reaction, a reaction product may be
purified by a known method (for example, high performance liquid
chromatography (HPLC)), as appropriate.
[0227] The haloacetamide complex-type sugar chain derivative is a
compound formed by substituting a hydroxyl group binding to the
carbon at position 1 of a complex-type asparagine-bound sugar chain
with --NH--(CO)--(CH.sub.2).sub.a--CH.sub.2X (wherein X represents
a halogen atom, and a represents an integer that is not
particularly limited unless it interferes a linker function of
interest, and preferably represents an integer from 0 to 4), for
example.
[0228] Specifically, the haloacetamide complex-type sugar chain
derivative is allowed to react with the above obtained peptide in a
phosphate buffer at a room temperature. After completion of the
reaction, the reaction solution is purified by HPLC to obtain a
sugar chain-added glycopeptide.
[0229] It is also possible to combine a sugar chain elongation
reaction using transferase with the aforementioned method. The thus
obtained glycopeptide is also included in the scope of the present
invention.
EXAMPLES
[0230] Hereinafter, the present invention will be specifically
described in the following examples. However, these examples are
not intended to limit the scope of the present invention.
Example 1
Production of Ac-Ala-Ser-Gly-Leu
[0231] Wang resin (manufactured by Merck) (100 .mu.mol) was placed
in a solid-phase synthesis column, and it was then fully washed
with methylene chloride (DCM) and DMF. Thereafter, the resultant
was fully swollen with DCM. Fmoc-Leu (0.50 mmol), MSNT (0.50 mmol),
and N-methylimidazole (0.375 mmol) were dissolved in DCM (2 mL),
and the obtained solution was then placed in the solid-phase
synthesis column, followed by stirring at 25.degree. C. for 1 hour.
It is to be noted that the volume of the DCM can also be set at 1.5
mL. After completion of the stirring operation, the resin was fully
washed with DCM and DMF, and the Fmoc group was treated with a 20%
piperidine/DMF solution (2 mL) for 15 minutes for deprotection. It
is to be noted that the present deprotection treatment can also be
carried out for 10 minutes. After washing with DMF, as for the
subsequent elongation of a peptide chain, amino acids were
successively condensed according to the below-mentioned method.
[0232] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1
mg, 0.50 mmol) were dissolved in DMF (1 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. It is to
be noted that the volume of the DMF can also be set at 2 mL. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (2 mL) for 20 minutes for
deprotection. It is to be noted that the present deprotection
treatment can also be carried out for 10 minutes. These operations
were repeated, so that amino acids were successively condensed. As
amino acids protected by the Fmoc groups, Fmoc-Gly (148.7 mg, 0.50
mmol), Fmoc-Cys(Trt) (292.9 mg, 0.50 mmol), and Fmoc-Ala (155.7 mg,
0.50 mmol) were used, and a 4-residue peptide having a protecting
group, Fmoc-Ala-Cys(Trt)-Gly-Leu (SEQ ID NO: 1), was obtained on
the solid-phase resin. Thereafter, the Fmoc group was treated with
a 20% piperidine/DMF solution (2 mL) on the resin for 20 minutes
for deprotection, and free amino groups were then protected by
acetyl using a 20% acetic anhydride/DMF solution (2 mL). It is to
be noted that the volume of the 20% acetic anhydride/DMF solution
can also be set at 1.7 mL. After washing with DMF and DCM, a
previously prepared reagent (TFA/water/phenol/thioanisole/EDT
(1,2-ethanedithiol)/TIPS=81.5/5/5/5/2.5/1) was added to such an
extent that the resin was sufficiently immersed therein, and it was
then stirred at 25.degree. C. for 2 hours. The resin was removed by
filtration, and the reaction solution was then concentrated under a
reduced pressure. The obtained residue was purified by HPLC (Vydac
column C8, 250.times.10 mm; developing solvents A: 0.1% TFA aqueous
solution; and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=85:15.fwdarw.50:50; 60 minutes; flow rate: 3.0 ml/min) to
obtain a 4-residue peptide having a protecting group,
Ac-Ala-Cys-Gly-Leu (SEQ ID NO: 2).
[0233] 30 mg (73 .mu.mol) of the obtained 4-residue peptide (SEQ ID
NO: 2) was placed in a round-bottom flask, and it was dissolved in
73 mL of a 0.25 M Tris-HCl buffer (pH 8.6; containing 6 M guanidine
hydrochloride solution and 3.3 mM EDTA) and 24 mL of acetonitrile.
Thereafter, methyl-4-nitrobenzenesulfonate (316 mg) was added to
the solution at 25.degree. C. Thirty minutes later, a 10% TFA
solution (7.3 mL) was added thereto, so that the pH of the solution
was adjusted to be pH 4. Thereafter, diethyl ether was added
thereto to carry out an extraction operation. After completion of
the concentration, the obtained residue was placed in an ODS column
to carry out purification, thereby obtaining 25 mg of a 4-residue
peptide having a protecting group, Ac-Ala-Cys(Me)-Gly-Leu (SEQ ID
NO: 3), wherein the sulfur atom of the cysteine residue was
methylated.
[0234] ESI-MS: Calcd for C.sub.17H.sub.30N.sub.4O.sub.6S:
[M+1H].sup.1+ 419.2, found, 419.1
[0235] 6.5 mg (15 .mu.mol) of the obtained 4-residue peptide (SEQ
ID NO: 3) wherein the sulfur atom of the cysteine residue had been
methylated was placed in an Eppendorf tube, and it was dissolved in
an 80% formic acid solution (6.5 mL). Thereafter, 159.0 mg (1.5
mmol) of cyanogen bromide was added to the solution at 25.degree.
C. The reactor was shaded from light, and a reaction was then
carried out at 37.degree. C. 28.5 hours later, the reaction
solution was frozen to terminate the reaction. The residue obtained
after the freeze-drying of the reaction solution was purified by
HPLC (Vydac column C4, 250.times.4.6 mm; developing solvents A:
0.1% TFA aqueous solution; and B: 0.08% TFA;
acetonitrile:water=90:10; gradients A:B=100:0.fwdarw.60:40; 30
minutes; flow rate: 1.0 ml/min) to obtain 3.8 mg of an ester form
as a reaction intermediate.
[0236] ESI-MS: Calcd for C.sub.16H.sub.28N.sub.4O.sub.7:
[M+1H].sup.1+ 389.4, found, 389.2
[0237] 5 mg of the obtained reaction intermediate was placed in an
Eppendorf tube in the same above manner, and it was dissolved in
0.6 mL of a phosphate buffer (pH 7.2; containing 6 M guanidine
hydrochloride), followed by a reaction at 37.degree. C. One hour
later, after confirming by HPLC that the reaction had been
terminated, the reaction solution was purified by HPLC (Vydac
column C4, 250.times.4.6 mm; developing solvents A: 0.1% TFA
aqueous solution; and B: 0.08% TFA; acetonitrile:water=90:10;
gradients A:B=100:0.fwdarw.60:40; 30 minutes; flow rate: 1.0
ml/min) to obtain 3.5 mg of a 4-residue peptide of interest having
a protecting group, Ac-Ala-Ser-Gly-Leu (SEQ ID NO: 4). It is to be
noted that the reaction time can also be set at 30 minutes.
[0238] ESI-MS: Calcd for C.sub.16H.sub.28N.sub.4O.sub.7:
[M+1H].sup.1+ 389.4, found, 389.1
Example 2
Production of Val-Asp-Lys-Ala-Val-Ser-Gly-Leu
[0239] Wang resin (manufactured by Merck) (100 .mu.mol) was placed
in a solid-phase synthesis column, and it was then fully washed
with methylene chloride (DCM) and DMF. Thereafter, the resultant
was fully swollen with DCM. Fmoc-Leu (0.50 mmol), MSNT (0.50 mmol),
and N-methylimidazole (0.375 mmol) were dissolved in DCM (2 mL),
and the obtained solution was then placed in the solid-phase
synthesis column, followed by stirring at 25.degree. C. for 1 hour.
It is to be noted that the volume of the DCM can also be set at 1.5
mL. After completion of the stirring operation, the resin was fully
washed with DCM and DMF, and the Fmoc group was treated with a 20%
piperidine/DMF solution (2 mL) for 15 minutes for deprotection. It
is to be noted that the present deprotection treatment can also be
carried out for 10 minutes. After washing with DMF, as for the
subsequent elongation of a peptide chain, amino acids were
successively condensed according to the below-mentioned method.
[0240] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1
mg, 0.50 mmol) were dissolved in DMF (1 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. It is to
be noted that the volume of the DMF can also be set at 2 mL. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (2 mL) for 20 minutes for
deprotection. It is to be noted that the present deprotection
treatment can also be carried out for 10 minutes. These operations
were repeated, so that amino acids were successively condensed. As
amino acids protected by the Fmoc groups, Fmoc-Gly (148.7 mg, 0.50
mmol), Fmoc-Cys (Trt) (292.9 mg, 0.50 mmol), Fmoc-Val (169.7 mg,
0.50 mmol), Fmoc-Ala (155.7 mg, 0.50 mmol), Fmoc-Lys (Boc) (234.3
mg, 0.50 mmol), Fmoc-Asp (OtBu) (205.8 mg, 0.50 mmol), and Fmoc-Val
(169.7 mg, 0.50 mmol) were used, and a 8-residue peptide having a
protecting group,
Fmoc-Val-Asp(OtBu)-Lys(Boc)-Ala-Val-Cys(Trt)-Gly-Leu (SEQ ID NO:
5), was obtained on the solid-phase resin. Thereafter, the Fmoc
group was treated with a 20% piperidine/DMF solution (2 mL) on the
resin for 20 minutes for deprotection, and the reaction solution
was then washed with DMF and DCM. Thereafter, a previously prepared
reagent K (TFA/water/phenol/thioanisole/EDT=82.5/5/5/5/2.5) was
added to such an extent that the resin was sufficiently immersed
therein, and it was then stirred at 25.degree. C. for 2 hours. It
is to be noted that, instead of the reagent K,
TFA/water/phenol/thioanisole/EDT/TIPS=81.5/5/5/5/2.5/1.0 may also
be used. The resin was removed by filtration, and the reaction
solution was then concentrated under a reduced pressure. The
obtained residue was purified by HPLC (Vydac column C18,
250.times.10 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=85:15.fwdarw.50:50; 15 minutes; flow rate: 2.5 ml/min) to
obtain an 8-residue peptide, Val-Asp-Lys-Ala-Val-Cys-Gly-Leu (SEQ
ID NO: 6).
[0241] 32 mg (40 .mu.mol) of the obtained 8-residue peptide (SEQ ID
NO: 6) was placed in a round-bottom flask, and it was dissolved in
40 mL of a 0.25 M Tris-HCl buffer (pH 8.6; containing 6 M guanidine
hydrochloride solution and 3.3 mM EDTA) and 13 mL of acetonitrile.
Thereafter, methyl-4-nitrobenzenesulfonate (261 mg) was added to
the solution at 25.degree. C. One hour later, a 10% TFA solution
(3.8 mL) was added thereto, so that the pH of the solution was
adjusted to be pH 4. Thereafter, diethyl ether was added thereto to
carry out an extraction operation. After completion of the
concentration, the obtained residue was placed in an ODS column to
carry out purification, thereby obtaining 30 mg of a 8-residue
peptide, Val-Asp-Lys-Ala-Val-Cys(Me)-Gly-Leu (SEQ ID NO: 7),
wherein the sulfur atom of the cysteine residue was methylated.
[0242] ESI-MS: Calcd for C.sub.35H.sub.64N.sub.9O.sub.11S:
[M+1H].sup.1+ 819.0, found, 818.8
[0243] 29 mg (36 .mu.mol) of the obtained 8-residue peptide (SEQ ID
NO: 7) wherein the sulfur atom of the cysteine residue had been
methylated was placed in a round-bottom flask, and it was dissolved
in 15 mL of an 80% formic acid solution. Thereafter, 381 mg (3.6
mmol) of cyanogen bromide was added to the solution at 25.degree.
C. The reactor was shaded from light, and a reaction was then
carried out at 25.degree. C. Thirty-two hours later, the reaction
solution was frozen to terminate the reaction. The residue obtained
after the freeze-drying of the reaction solution was purified by
HPLC (Vydac column C8, 250.times.10 mm; developing solvents A: 0.1%
TFA aqueous solution; and B: 0.08% TFA; acetonitrile:water=90:10;
gradients A:B=90:10.fwdarw.70:30; 60 minutes; flow rate: 4.0
ml/min) to obtain 18 mg of an ester form as a reaction
intermediate.
[0244] ESI-MS: Calcd for C.sub.34H.sub.62N.sub.9O.sub.12:
[M+1H].sup.1+ 788.9, found, 788.5
[0245] 5 mg of the obtained reaction intermediate was placed in an
Eppendorf tube, and it was dissolved in 0.63 mL of a phosphate
buffer (pH 7.2; containing 6 M guanidine hydrochloride), followed
by a reaction at 37.degree. C. 9.25 hours later, after confirming
that the reaction had been terminated, the reaction solution was
purified by HPLC (Vydac column C4, 250.times.4.6 mm; developing
solvents A: 0.1% TFA aqueous solution; and B: 0.08% TFA;
acetonitrile:water=90:10; gradients A:B=100:0.fwdarw.40:60; 30
minutes; flow rate: 1.0 ml/min) to obtain 4.1 mg of a 8-residue
peptide of interest, Val-Asp-Lys-Ala-Val-Ser-Gly-Leu (SEQ ID NO:
8). It is to be noted that the reaction time can also be set at
7.25 hours.
[0246] ESI-MS: Calcd for C.sub.34H.sub.62N.sub.9O.sub.12:
[M+1H].sup.1+ 788.9, found, 788.7
Example 3
Production of Leu-Phe-Arg-Val-Tyr-Ser-Asn-Phe-Leu-Arg-Gly
[0247] Wang resin (manufactured by Merck) (100 .mu.mol) was placed
in a solid-phase synthesis column, and it was then fully washed
with methylene chloride (DCM) and DMF. Thereafter, the resultant
was fully swollen with DCM. Fmoc-Gly (0.50 mmol), MSNT (0.50 mmol),
and N-methylimidazole (0.375 mmol) were dissolved in DCM (2 mL),
and the obtained solution was then placed in the solid-phase
synthesis column, followed by stirring at 25.degree. C. for 1 hour.
It is to be noted that the volume of the DCM can also be set at 1.5
mL. After completion of the stirring operation, the resin was fully
washed with DCM and DMF, and the Fmoc group was treated with a 20%
piperidine/DMF solution (2 mL) for 15 minutes for deprotection. It
is to be noted that the present deprotection treatment can also be
carried out for 10 minutes. After washing with DMF, as for the
subsequent elongation of a peptide chain, amino acids were
successively condensed according to the below-mentioned method.
[0248] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1
mg, 0.50 mmol) were dissolved in DMF (1 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (2 mL) for 20 minutes for
deprotection. It is to be noted that the present deprotection
treatment can also be carried out for 10 minutes. These operations
were repeated, so that amino acids were successively condensed. As
amino acids protected by the Fmoc groups, Fmoc-Arg (Pbf) (324.4 mg,
0.50 mmol), Fmoc-Leu (176.7 mg, 0.50 mmol), Fmoc-Phe (193.7 mg,
0.50 mmol), Fmoc-Asn (177.2 mg, 0.50 mmol), Fmoc-Cys (Trt) (292.9
mg, 0.50 mmol), Fmoc-Tyr (tBu) (229.8 mg, 0.50 mmol), Fmoc-Val
(169.7 mg, 0.50 mmol), Fmoc-Arg (Pbf) (324.4 mg, 0.50 mmol),
Fmoc-Phe (193.7 mg, 0.50 mmol), and Fmoc-Leu (176.7 mg, 0.50 mmol)
were used, and a 11-residue peptide having a protecting group,
Fmoc-Leu-Phe-Arg(Pbf)-Val-Tyr(tBu)-Cys(Trt)-Asn-Phe-Leu-Arg(Pbf)-Gly
(SEQ ID NO: 9), was obtained on the solid-phase resin. Thereafter,
the Fmoc group was treated with 2 mL of a 20% piperidine/DMF
solution on the resin for 20 minutes for deprotection. It is to be
noted that the present deprotection treatment can also be carried
out for 10 minutes. The reaction solution was washed with DMF and
DCM. Thereafter, a previously prepared reagent K
(TFA/water/phenol/thioanisole/EDT=82.5/5/5/5/2.5) was added to such
an extent that the resin was sufficiently immersed therein, and it
was then stirred at 25.degree. C. for 2 hours. It is to be noted
that, instead of the reagent K,
TFA/water/phenol/thioanisole/EDT/TIPS=81.5/5/5/5/2.5/1.0 may also
be used. The resin was removed by filtration, and the reaction
solution was then concentrated under a reduced pressure. The
obtained residue was purified by HPLC (Vydac column C18,
250.times.10 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=70:30.fwdarw.40:60; 15 minutes; flow rate: 2.0 ml/min) to
obtain a 11-residue peptide,
Leu-Phe-Arg-Val-Tyr-Cys-Asn-Phe-Leu-Arg-Gly (SEQ ID NO: 10).
[0249] 21 mg (15 .mu.mol) of the obtained 11-residue peptide (SEQ
ID NO: 10) was placed in a round-bottom flask, and it was dissolved
in 15 mL of a 0.25 M Tris-HCl buffer (pH 8.6; containing 6 M
guanidine hydrochloride solution and 3.3 mM EDTA) and acetonitrile
(5 mL). Thereafter, methyl-4-nitrobenzenesulfonate (66 mg) was
added to the solution at 25.degree. C. Thirty minutes later, a 10%
TFA solution (1.5 mL) was added thereto, so that the pH of the
solution was adjusted to be pH 4. Thereafter, diethyl ether was
added thereto to carry out an extraction operation. After
completion of the concentration, the obtained residue was placed in
an ODS column to carry out purification, thereby obtaining 19 mg of
a 11-residue peptide,
Leu-Phe-Arg-Val-Tyr-Cys(Me)-Asn-Phe-Leu-Arg-Gly (SEQ ID NO: 11),
wherein the sulfur atom of the cysteine residue was methylated.
[0250] ESI-MS: Calcd for C.sub.66H.sub.100N.sub.18O.sub.14S:
[M+2H].sup.2+701.4, found, 701.5
[0251] 18 mg (13 .mu.mol) of the obtained 11-residue peptide (SEQ
ID NO: 11) wherein the sulfur atom of the cysteine residue had been
methylated was placed in a round-bottom flask, and it was dissolved
in 5.4 mL of a 2.4 mM 80% formic acid solution. Thereafter, 136 mg
(1.3 mmol) of cyanogen bromide was added to the solution at
25.degree. C. The reactor was shaded from light, and a reaction was
then carried out at 25.degree. C. Approximately fifty hours later,
the reaction solution was frozen to terminate the reaction. The
residue obtained after the freeze-drying of the reaction solution
was purified by HPLC (Vydac column C8, 250.times.10 mm; developing
solvents A: 0.1% TFA aqueous solution; and B: 0.08% TFA;
acetonitrile:water=90:10; gradients A:B=85:15.fwdarw.50:50; 60
minutes; flow rate: 3.0 ml/min) to obtain 7.5 mg of an ester form
as a reaction intermediate.
[0252] ESI-MS: Calcd for C.sub.65H.sub.100N.sub.18O.sub.15:
[M+2H].sup.2+686.4, found, 686.5
[0253] 7 mg of the obtained reaction intermediate was placed in an
Eppendorf tube, and it was dissolved in 0.50 mL of a phosphate
buffer (pH 7.2; containing 6 M guanidine hydrochloride), followed
by a reaction at 37.degree. C. One hour later, after confirming
that the reaction had been terminated, the reaction solution was
purified by HPLC (Vydac column C4, 250.times.4.6 mm; developing
solvents A: 0.1% TFA aqueous solution; and B: 0.08% TFA;
acetonitrile:water=90:10; gradients A:B=80:20.fwdarw.40:60; 30
minutes; flow rate: 1.0 ml/min) to obtain 5.4 mg of a 11-residue
peptide of interest, Leu-Phe-Arg-Val-Tyr-Ser-Asn-Phe-Leu-Arg-Gly
(SEQ ID NO: 12).
[0254] ESI-MS: Calcd for C.sub.65H.sub.100N.sub.18O.sub.15:
[M+2H].sup.2+686.4, found, 686.4
Example 4
Production of Ala-Leu-Leu-Val-Asn(Oligosaccharide
chain)-Ser-Ser-Gln-Pro-Trp-Glu-Pro-Leu-Gln-Leu-His-Val-Asp-Lys-Ala
[0255] Amino-PEGA resin (manufactured by Merck) (100 .mu.mol) was
placed in a solid-phase synthesis column, and it was then fully
washed with methylene chloride (DCM) and DMF. Thereafter, the
resultant was fully swollen with DCM.
4-hydroxymethyl-3-methoxyphenoxy butyric acid (HMPB) (0.25 mmol),
TBTU (0.25 mmol), and N-ethylmorpholine (0.25 mmol) were dissolved
in DMF (2 mL), and the obtained solution was then placed in the
column, followed by stirring at 25.degree. C. for 4 hours.
Thereafter, the resin was fully washed with DMF and DCM to obtain
an HMPB-PEGA resin. The obtained HMPB-PEGA resin was used as a
solid-phase carrier in solid-phase synthesis.
[0256] Fmoc-Ser (tBu) (0.50 mmol), MSNT (0.50 mmol), and
N-methylimidazole (0.375 mmol) were dissolved in DCM (2 ml), and
the obtained solution was then placed in the solid-phase synthesis
column, followed by a reaction at 25.degree. C. for 3 hours. It is
to be noted that the volume of the DCM can also be set at 2.5 mL.
After completion of the stirring operation, the resin was washed
with DCM and DMF, and the Fmoc group was treated with a 20%
piperidine/DMF solution (2 mL) for 15 minutes for deprotection. It
is to be noted that the present deprotection treatment can also be
carried out for 10 minutes.
[0257] After washing with DMF, a resin corresponding to 2 .mu.mol
of 1-residue peptide was transferred into an Eppendorf tube. The
sugar chain asparagine (10 mg, 3.6 .mu.mol) represented by Formula
(9) below and DEPBT (2 mg, 6 .mu.mol) were dissolved in DMF (0.12
mL), and the obtained solution was then placed in an Eppendorf
tube. DIPEA (0.68 .mu.l, 4 .mu.mol) was added thereto, and the
obtained mixture was then stirred at 25.degree. C. for 18
hours.
##STR00015##
[0258] After completion of the stirring operation, the resin was
washed with DCM and DMF. The Fmoc group was treated with a 20%
piperidine/DMF solution (1 mL) for 15 minutes for deprotection.
After washing with DMF, as for the subsequent elongation of a
glycopeptide chain, amino acids were successively condensed
according to the below-mentioned method.
[0259] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (1.35 mg, 0.01 mmol), and DIPCl (1.53 .mu.L, 1.26
mg, 0.01 mmol) were dissolved in DMF (0.02 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (1 mL) for 20 minutes for
deprotection. These operations were repeated, so that amino acids
were successively condensed. As amino acids whose amino groups had
been protected by the Fmoc groups, Fmoc-Val (3.4 mg, 0.01 mmol),
Fmoc-Leu (3.5 mg, 0.01 mmol), Fmoc-Leu (3.5 mg, 0.01 mmol), and
Fmoc-Ala (3.1 mg, 0.01 mmol) were used, and a 6-residue sugar-added
peptide having a protecting group,
Fmoc-Ala-Leu-Leu-Val-Asn(Oligosaccharide chain)-Ser(tBu) (SEQ ID
NO: 13), was obtained on the solid-phase resin. Acetic
acid:trifluoroethanol (=1:1) was added to this sugar-added peptide
to such an extent that the resin was sufficiently immersed therein.
Four hours later, the resin was removed by filtration, and a
filtrate portion was then added to diethyl ether, which had been
prepared separately to carry out crystallization. Thereafter, the
solution portion was removed using a membrane filter, thereby
obtaining a residue containing a 6-residue sugar chain-added
peptide having a protecting group (SEQ ID NO: 13).
[0260] 2 mg (0.55 .mu.mol) of the obtained 6-residue sugar
chain-added peptide having a protecting group (SEQ ID NO: 13),
molecular sieve (MS) 4A (10 mg), and benzylmercaptan (2 .mu.l, 16.4
.mu.mol) were stirred in a DMF solvent (85 .mu.l) in an argon
atmosphere at -20.degree. C. for 1 hour. Thereafter, PyBOP (1.4 mg,
2.7 .mu.mol) and DIPEA (0.46 .mu.l, 2.7 .mu.mol) were added to the
reaction solution, and the obtained mixture was then stirred for
2.5 hours. Thereafter, diethyl ether (5 ml) was added to the
reaction solution to precipitate a compound. The precipitated
compound was filtrated, and the precipitate was then recovered with
a 50% acetonitrile aqueous solution. The recovered product was
freeze-dried, and a 95% TFA aqueous solution was added to the
obtained freeze-dried product. The obtained mixture was stirred at
25.degree. C. for 2 hours. The resin was removed by filtration. The
reaction solution was concentrated and was then dissolved in a 50%
acetonitrile aqueous solution, followed by freeze-drying. The
freeze-dried product was purified by HPLC (Vydac column C4,
250.times.4.6 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=80:20.fwdarw.40:60; 60 minutes; flow rate: 1.0 ml/min) to
obtain a 6-residue sugar chain-added peptide having benzylthioester
at the C-terminus thereof, Ala-Leu-Leu-Val-Asn(Oligosaccharide
chain)-Ser-SBn (SEQ ID NO: 15).
[0261] The following method can also be applied as the
aforementioned step of obtaining the 6-residue sugar chain-added
peptide shown in SEQ ID NO: 15 from the reaction with sugar chain
asparagine in an Eppendorf tube:
[0262] After washing with DMF, a resin corresponding to 4.3 .mu.mol
of 1-residue peptide was transferred into an Eppendorf tube. The
sugar chain asparagine (17 mg, 8.6 .mu.mol) represented by Formula
(9) and DEPBT (6 mg, 8.6 .mu.mol) were dissolved in DMF:DMSO=4:1
(0.29 mL), and the obtained solution was then placed in an
Eppendorf tube. DIPEA (1.5 .mu.l, 8.6 .mu.mol) was added thereto,
and the obtained mixture was then stirred at 25.degree. C. for 18
hours.
[0263] After completion of the stirring operation, the resin was
washed with DCM and DMF. The Fmoc group was treated with a 20%
piperidine/DMF solution (1 mL) for 10 minutes for deprotection.
After washing with DMF, as for the subsequent elongation of a
glycopeptide chain, amino acids were successively condensed
according to the below-mentioned method.
[0264] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (2.9 mg, 0.022 mmol), and DIPCl (3.3 .mu.L, 0.022
mmol) were dissolved in DMF (0.54 mL), and the obtained solution
was then activated for 15 minutes. Thereafter, the solution was
placed in the solid-phase synthesis column. After stirring at
25.degree. C. for 1 hour, the Fmoc group was treated with a 20%
piperidine/DMF solution (1 mL) for 20 minutes for deprotection.
These operations were repeated, so that amino acids were
successively condensed. As amino acids whose amino groups had been
protected by the Fmoc groups, Fmoc-Val (7.3 mg, 0.022 mmol),
Fmoc-Leu (7.6 mg, 0.022 mmol), Fmoc-Leu (7.6 mg, 0.022 mmol), and
Boc-Ala (4.0 mg, 0.022 mmol) were used, and a 6-residue sugar-added
peptide having a protecting group,
Boc-Ala-Leu-Leu-Val-Asn(Oligosaccharide chain)-Ser(tBu) (SEQ ID NO:
14), was obtained on the solid-phase resin. Acetic
acid:trifluoroethanol (=1:1) was added to this sugar-added peptide
to such an extent that the resin was sufficiently immersed therein.
Twenty-four hours later, the resin was removed by filtration, and a
filtrate portion was then added to diethyl ether, which had been
prepared separately to carry out crystallization. Thereafter, the
solution portion was removed using a membrane filter, thereby
obtaining a residue containing a 6-residue sugar chain-added
peptide having a protecting group (SEQ ID NO: 14).
[0265] 18 mg (7.5 .mu.mol) of the obtained 6-residue sugar
chain-added peptide having a protecting group (SEQ ID NO: 14),
molecular sieve (MS) 4A (190 mg), and benzylmercaptan (26 .mu.l,
37.5 .mu.mol) were stirred in a DMF solvent (1.9 ml) in an argon
atmosphere at -20.degree. C. for 1 hour. Thereafter, PyBOP (20 mg,
37.5 .mu.mol) and DIPEA (6.7 .mu.l, 37.5 .mu.mol) were added to the
reaction solution, and the obtained mixture was then stirred for 2
hours. Thereafter, diethyl ether (10 ml) was added to the reaction
solution to precipitate a compound. The precipitated compound was
filtrated, and the precipitate was then dissolved in DMF. The
obtained solution was concentrated under a reduced pressure, and a
95% TFA aqueous solution was added to the obtained residue. The
obtained mixture was stirred at 25.degree. C. for 2 hours. The
reaction solution was concentrated and was then purified by HPLC
(Vydac column C4, 250.times.4.6 mm; developing solvents A: 0.1% TFA
aqueous solution; and B: 0.08% TFA; acetonitrile:water=90:10;
gradients A:B=100:0.fwdarw.40:60; 60 minutes; flow rate: 1.0
ml/min) to obtain a 6-residue sugar chain-added peptide having
benzylthioester at the C-terminus thereof,
Ala-Leu-Leu-Val-Asn(Oligosaccharide chain)-Ser-SBn (SEQ ID NO:
15).
[0266] On the other hand, Wang resin (manufactured by Merck) (100
.mu.mol) was placed in a solid-phase synthesis column, and it was
then fully washed with methylene chloride (DCM) and DMF.
Thereafter, the resultant was fully swollen with DCM. Fmoc-Ala
(0.50 mmol), MSNT (0.50 mmol), and N-methylimidazole (0.375 mmol)
were dissolved in DCM (2 mL), and the obtained solution was then
placed in the solid-phase synthesis column, followed by stirring at
25.degree. C. for 1 hour. It is to be noted that the volume of the
DCM can also be set at 1.5 mL. After completion of the stirring
operation, the resin was fully washed with DCM and DMF, and the
Fmoc group was treated with a 20% piperidine/DMF solution (2 mL)
for 15 minutes for deprotection. It is to be noted that the present
deprotection treatment can also be carried out for 10 minutes.
After washing with DMF, as for the subsequent elongation of a
peptide chain, amino acids were successively condensed according to
the below-mentioned method.
[0267] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1
mg, 0.50 mmol) were dissolved in DMF (1 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. It is to
be noted that the volume of the DMF can also be set at 2 mL. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (2 mL) for 20 minutes for
deprotection. These operations were repeated, so that amino acids
were successively condensed. As amino acids protected by the Fmoc
groups, Fmoc-Lys (Boc) (234.3 mg, 0.50 mmol), Fmoc-Asp (OtBu)
(205.8 mg, 0.50 mmol), Fmoc-Val (169.7 mg, 0.50 mmol), Fmoc-His
(Trt) (309.9 mg, 0.50 mmol), Fmoc-Leu (176.7 mg, 0.50 mmol),
Fmoc-Gln (184.2 mg, 0.50 mmol), Fmoc-Leu (176.7 mg, 0.50 mmol),
Fmoc-Pro (168.7 mg, 0.50 mmol), Fmoc-Glu (OtBu) (212.8 mg, 0.50
mmol), Fmoc-Trp (Boc) (263.3 mg, 0.50 mmol), Fmoc-Pro (168.7 mg,
0.50 mmol), Fmoc-Gln (184.2 mg, 0.50 mmol), and Fmoc-Cys (Trt)
(292.9 mg, 0.50 mmol) were used, and a 14-residue peptide having a
protecting group,
Fmoc-Cys(Trt)-Gln-Pro-Trp(Boc)-Glu(OtBu)-Pro-Leu-Gln-Leu-His(Trt)-Val-Asp-
(OtBu)-Lys(Boc)-Ala (SEQ ID NO: 16), was obtained on the
solid-phase resin. Thereafter, the Fmoc group was treated with a
20% piperidine/DMF solution (2 mL) for 20 minutes for deprotection,
the resultant was then washed with DMF and DCM. Thereafter, a
previously prepared reagent K
(TFA/water/phenol/thioanisole/EDT=82.5/5/5/5/2.5) was added to such
an extent that the resin was sufficiently immersed therein, and it
was then stirred at 25.degree. C. for 2 hours. It is to be noted
that, instead of the reagent K,
TFA/water/phenol/thioanisole/EDT/TIPS=81.5/5/5/5/2.5/1.0 may also
be used. The resin was removed by filtration, and the reaction
solution was then concentrated under a reduced pressure. The
obtained residue was purified by HPLC (Vydac column C8,
250.times.10 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=80:20.fwdarw.40:60; 30 minutes; flow rate: 4.0 ml/min) to
obtain a 14-residue peptide,
Cys-Gln-Pro-Trp-Glu-Pro-Leu-Gln-Leu-His-Val-Asp-Lys-Ala (SEQ ID NO:
17).
[0268] 1.1 mg of the thus prepared 14-residue peptide (SEQ ID NO:
17) and 1.3 mg of the previously synthesized 6-residue sugar-added
peptide having benzylthioester at the C-terminus thereof (SEQ ID
NO: 15) were placed in a single Eppendorf tube, and they were
dissolved in 275 .mu.L of a phosphate buffer (pH 7.2; containing 6
M guanidine hydrochloride). Thereafter, thiophenol (1 .mu.L) and
benzylmercaptan (1 .mu.L) were added to the solution at 25.degree.
C., and a reaction was then carried out at 37.degree. C. Twenty-six
hours later, after confirming by HPLC that the reaction had been
terminated, the reaction solution was purified by HPLC (Vydac
column C18, 250.times.4.6 mm; developing solvents A: 0.1% TFA
aqueous solution; and B: 0.08% TFA; acetonitrile:water=90:10;
gradients A:B=80:20.fwdarw.50:50; 60 minutes; flow rate: 1.0
ml/min) to obtain 1.5 mg of a 20-residue sugar chain-added peptide,
Ala-Leu-Leu-Val-Asn(Oligosaccharide
chain)-Ser-Cys-Gln-Pro-Trp-Glu-Pro-Leu-Gln-Leu-His-Val-Asp-Lys-Ala
(SEQ ID NO: 18).
[0269] 1.5 mg (0.39 .mu.mol) of the obtained 20-residue sugar
chain-added peptide (SEQ ID NO: 18) was placed in a round-bottom
flask, and it was dissolved in 0.39 mL of a 0.25 M Tris-HCl buffer
(pH 8.6; containing 6 M guanidine hydrochloride solution and 3.3 mM
EDTA) and 0.13 mL of acetonitrile. Thereafter,
methyl-4-nitrobenzenesulfonate (1.7 mg) was added to the solution
at 25.degree. C. Forty minutes later, a 10% TFA solution (1.5 mL)
was added thereto, so that the pH of the solution was adjusted to
be pH 4. Thereafter, diethyl ether was added thereto to carry out
an extraction operation. After a water layer had been concentrated,
the obtained residue was purified by HPLC (Vydac column C4,
250.times.4.6 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=80:20.fwdarw.55:45; 60 minutes; flow rate: 1.0 ml/min) (wherein
a C18 column can also be used in the purification, instead of the
aforementioned C4 column) to obtain 1.6 mg of a 20-residue sugar
chain-added peptide, Ala-Leu-Leu-Val-Asn(Oligosaccharide
chain)-Ser-Cys(Me)-Gln-Pro-Trp-Glu-Pro-Leu-Gln-Leu-His-Val-Asp-Lys-Ala
(SEQ ID NO: 19), wherein the sulfur atom of the cysteine residue
was methylated.
[0270] ESI-MS: Calcd for C.sub.165H.sub.265N.sub.31O.sub.74S:
[M+2H].sup.2+1300.7, found, 1300.4
[0271] 1.6 mg (0.4 .mu.mol) of the obtained 20-residue sugar
chain-added peptide (SEQ ID NO: 19) wherein the sulfur atom of the
cysteine residue had been methylated was placed in an Eppendorf
tube, and it was dissolved in 0.4 mL of a 80% formic acid solution.
Thereafter, 4.3 mg (0.04 mmol) of cyanogen bromide was added to the
solution at 25.degree. C. The reactor was shaded from light, and a
reaction was then carried out at 37.degree. C. Thirty-five hours
later, the reaction solution was frozen to terminate the reaction.
A 5% hydrazine hydrate (200 .mu.L) was added to the residue
obtained after the freeze-drying of the reaction solution, and the
inside of the reaction system was adjusted to be pH 10 to 11. Five
minutes later, acetic acid (5 .mu.L) was added to the reaction
system, so that the inside thereof was adjusted to pH 8 to 9. The
resultant was purified by HPLC (Vydac column C4, 250.times.4.6 mm;
developing solvents A: 0.1% TFA aqueous solution; and B: 0.08% TFA;
acetonitrile:water=90:10; gradients A:B=80:20.fwdarw.50:50; 30
minutes; flow rate: 1.0 ml/min) to obtain 0.7 mg of a 20-residue
sugar chain-added peptide, Ala-Leu-Leu-Val-Asn(Oligosaccharide
chain)-Ser-Ser-Gln-Pro-Trp-Glu-Pro-Leu-Gln-Leu-His-Val-Asp-Lys-Ala
(SEQ ID NO: 20), which was a glycopeptide of interest.
[0272] Thirty-four hours after a reaction with cyanogen bromide,
the reaction solution was concentrated under a reduced pressure,
and a 5% hydrazine hydrate (200 .mu.L) was added to the obtained
residue, followed by stirring for 10 minutes. Thereafter, 5 .mu.L
of acetic acid was added to the reaction solution, and the obtained
mixture was then purified by HPLC. In this case also, the same
20-residue sugar chain-added peptide (SEQ ID NO: 20) could be
obtained.
[0273] ESI-MS: Calcd for C.sub.164H.sub.263N.sub.31O.sub.75:
[M+2H].sup.2+1290.6, found, 1290.7
Example 5
Production of Ac-Gly-Ser-Gly-Met-Ala
[0274] Wang resin (manufactured by Merck) (100 .mu.mol) was placed
in a solid-phase synthesis column, and it was then fully washed
with methylene chloride (DCM) and DMF. Thereafter, the resultant
was fully swollen with DCM. Fmoc-Ala (0.50 mmol), MSNT (0.50 mmol),
and N-methylimidazole (0.375 mmol) were dissolved in DCM (1.5 mL),
and the obtained solution was then placed in the solid-phase
synthesis column, followed by stirring at 25.degree. C. for 2
hours. After completion of the stirring operation, the resin was
fully washed with DCM and DMF, and the Fmoc group was treated with
a 20% piperidine/DMF solution (2 mL) for 15 minutes for
deprotection. After washing with DMF, as for the subsequent
elongation of a peptide chain, amino acids were successively
condensed according to the below-mentioned method.
[0275] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1
mg, 0.50 mmol) were dissolved in DMF (2 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (2 mL) for 10 minutes for
deprotection. These operations were repeated, so that amino acids
were successively condensed. As amino acids protected by the Fmoc
groups, Fmoc-Met(0) (193.8 mg, 0.50 mmol), Fmoc-Gly (148.9 mg, 0.50
mmol), Fmoc-Cys(Trt) (292.9 mg, 0.50 mmol), and Fmoc-Gly (148.9 mg,
0.50 mmol) were used, and a 5-residue peptide having a protecting
group, Fmoc-Gly-Cys(Trt)-Gly-Met(0)-Ala (SEQ ID NO: 21), was
obtained on the solid-phase resin. Thereafter, the Fmoc group was
treated with a 20% piperidine/DMF solution (2 mL) on the resin for
10 minutes for deprotection, and free amino groups were then
protected by acetyl using a 20% acetic anhydride/DMF solution (1.25
mL). After washing with DMF and DCM, a previously prepared reagent
(TFA/water/TIPS=95/2.5/2.5) was added to such an extent that the
resin was sufficiently immersed therein, and it was then stirred at
25.degree. C. for 2 hours. The resin was removed by filtration, and
20 mL of diethyl ether was then added to the filtrate for
precipitation. The precipitate was filtrated, and the filtrate was
then dissolved in a 0.1% TFA aqueous solution. The obtained
solution was concentrated under a reduced pressure, and the
resultant was then freeze-dried to obtain a mixture comprising a
5-residue peptide, Ac-Gly-Cys-Gly-Met(0)-Ala (SEQ ID NO: 22),
wherein the sulfur atom of the methionine at position 4 was
oxidized.
[0276] 5 mg of the obtained 5-residue peptide (SEQ ID NO: 22)
mixture, wherein the sulfur atom of the methionine at position 4
had been oxidized, was placed in a round-bottom flask, and it was
dissolved in 10 mL of a 0.25 M Tris-HCl buffer (pH 8.6; containing
6 M guanidine hydrochloride solution and 3.3 mM EDTA).
2-mercaptoethanol (7 .mu.L) was added to the obtained solution, and
the obtained mixture was then stirred for 10 minutes. Thereafter,
3.3 mL of an acetonitrile solution containing
methyl-4-nitrobenzenesulfonate (66 mg) was added to the reaction
solution. Twenty-five minutes later, a 10% TFA solution (1.0 mL)
was added thereto for neutralization. Thereafter, diethyl ether was
added thereto to carry out an extraction operation 3 times. After a
water layer had been concentrated under a reduced pressure, the
obtained residue was purified by HPLC (Vydac column C18,
250.times.10 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.09% TFA; acetonitrile:water=90:10; gradients
A:B=100:0.fwdarw.100:0.fwdarw.60:40; 0 minute.fwdarw.5
minutes.fwdarw.35 minutes; flow rate: 4.0 ml/min) to obtain 4 mg of
a 5-residue peptide, Ac-Gly-Cys(Me)-Gly-Met(0)-Ala (SEQ ID NO: 23),
wherein the sulfur atom of the cysteine residue at position 2 was
methylated and the methionine residue at position 4 was
oxidized.
[0277] 3.8 mg of the obtained 5-residue peptide (SEQ ID NO: 23),
wherein the sulfur atom of the cysteine residue at position 2 had
been methylated and the methionine residue at position 4 had been
oxidized, was placed in an Eppendorf tube, and it was dissolved in
3.1 mL of a 80% formic acid solution. Thereafter, 79.0 mg of
cyanogen bromide was added to the solution, and the reactor was
then shaded from light. The reaction solution was stirred under
argon-substituted conditions at 37.degree. C. for 39 hours. After
completion of the reaction, the resultant was subjected to vacuum
concentration to obtain a residue containing an ester form as a
reaction intermediate.
[0278] The obtained residue was placed in an Eppendorf tube, and it
was dissolved in 1.25 mL of a sodium phosphate buffer (containing 6
M guanidine hydrochloride; pH 7.2), followed by stirring for 45
minutes. Thereafter, 3.6 mL of trifluoroacetic acid, 22 mg of
ammonium iodide, and 11 .mu.L of dimethyl sulfide were added to the
reaction solution, and the obtained mixture was then stirred at a
room temperature. Thirty minutes later, 10 mL of water was added to
the reaction solution, and the obtained solution was then separated
and washed with carbon tetrachloride. A water layer was
concentrated under a reduced pressure, and the obtained residue was
then purified by HPLC (Vydac column C18, 250.times.4.6 mm;
developing solvents A: 0.1% TFA aqueous solution; and B: 0.09% TFA;
acetonitrile:water=90:10; gradients
A:B=100:0.fwdarw.100:0.fwdarw.60:40; 0 minute.fwdarw.>5
minutes.fwdarw.65 minutes; flow rate: 1.0 ml/min) to obtain 2.6 mg
of a 5-residue peptide of interest having a protecting group,
Ac-Gly-Ser-Gly-Met-Ala (SEQ ID NO: 24).
[0279] ESI-MS: Calcd for C.sub.16H.sub.28N.sub.4O.sub.7:
[M+1H].sup.1+ 464.2, found, 464.5
Example 6
Production of
Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-Asp-Thr-Arg-P-
ro-Ala-Pro-Gly-Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-
-Asp-Thr-Arg-Pro-Ala-Pro-Gly
[0280] Amino-PEGA resin (manufactured by Merck) (100 .mu.mol) was
placed in a solid-phase synthesis column, and it was then fully
washed with methylene chloride (DCM) and DMF. Thereafter, the
resultant was fully swollen with DMF.
4-hydroxymethyl-3-methoxyphenoxy butyric acid (HMPB) (0.25 mmol),
TBTU (0.25 mmol), and N-ethylmorpholine (0.25 mmol) were dissolved
in DMF (2 mL), and the obtained solution was then placed in the
column, followed by stirring at 25.degree. C. for 2 hours.
Thereafter, the resin was fully washed with DMF and DCM to obtain
an HMPB-PEGA resin. The obtained HMPB-PEGA resin was used as a
solid-phase carrier in solid-phase synthesis.
[0281] Fmoc-Gly (0.50 mmol), MSNT (0.50 mmol), and
N-methylimidazole (0.375 mmol) were dissolved in DCM (4.5 ml), and
the obtained solution was then placed in the solid-phase synthesis
column, followed by stirring at 25.degree. C. for 3 hours. After
completion of the stirring operation, the resin was washed with DCM
and DMF, and the Fmoc group was treated with a 20% piperidine/DMF
solution (2 mL) for 10 minutes for deprotection. After washing with
DMF, as for the subsequent elongation of a peptide chain, amino
acids were successively condensed according to the below-mentioned
method.
[0282] An amino acid whose amino group had been protected by an
Fmoc group, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1
mg, 0.50 mmol) were dissolved in DMF (4 mL), and the obtained
solution was then activated for 15 minutes. Thereafter, the
solution was placed in the solid-phase synthesis column. After
stirring at 25.degree. C. for 1 hour, the Fmoc group was treated
with a 20% piperidine/DMF solution (2 mL) for 10 minutes for
deprotection. These operations were repeated, so that amino acids
were successively condensed. As amino acids protected by the Fmoc
groups, Fmoc-Pro, Fmoc-Ala, Fmoc-Pro, Fmoc-Arg (Pbf), Fmoc-Thr
(tBu), Fmoc-Asp (OtBu), Fmoc-Pro, Fmoc-Ala, Fmoc-Ser (tBu),
Fmoc-Thr (tBu), Fmoc-Val, Fmoc-Gly, Fmoc-His (Trt), Fmoc-Ala,
Fmoc-Pro, Fmoc-Pro, and Fmoc-Ala were used. On the solid-phase
resin, a 18-residue peptide having a protecting group,
Fmoc-Ala-Pro-Pro-Ala-His(Trt)-Gly-Val-Thr(tBu)-Ser(tBu)-Ala-Pro-Asp(OtBu)-
-Thr(tBu)-Arg(Pbf)-Pro-Ala-Pro-Gly (SEQ ID NO: 25), was
obtained.
[0283] This 18-residue peptide having a protecting group (SEQ ID
NO: 25) was treated with a 20% piperidine/DMF solution (2 mL) on
the resin for 10 minutes for deprotection of the Fmoc group. After
the resultant had been washed with DMF and DCM, Fmoc-Thr(GalNAc)
(0.20 mmol), HOBt (0.50 mmol), and DIPCl (0.50 mmol) that had been
dissolved in DMF (3.6 mL) and had been activated for 15 minutes
were added to the resultant. After the reaction for 5 hours, the
Fmoc group was treated with a 20% piperidine/DMF solution (2 mL)
for 20 minutes for deprotection to obtain a 19-residue sugar-added
peptide having a protecting group,
Thr(GalNAc)-Ala-Pro-Pro-Ala-His(Trt)-Gly-Val-Thr(tBu)-Ser(tBu)-Ala-Pro-As-
p(OtBu)-Thr(tBu)-Arg(Pbf)-Pro-Ala-Pro-Gly (SEQ ID NO: 26), on the
solid-phase resin.
[0284] 0.02 mmol out of the obtained 19-residue sugar-added peptide
having a protecting group (SEQ ID NO: 26) on the solid-phase resin
was transferred into another solid-phase synthesis column.
Boc-Ser(tBu) (0.1 mmol), DIPCl (0.1 mmol), and HOBt (0.1 mmol) that
had been dissolved in 0.5 mL of DMF and had been activated for 15
minutes were added to the column, and a reaction was then carried
out for 1 hour. Thereafter, the reaction solution was filtrated,
and acetic acid:trifluoroethanol=1:1 were added thereto to such an
extent that the resin was sufficiently immersed therein. Fourteen
hours later, the resin was removed by filtration, and the filtrate
was then concentrated under a reduced pressure to obtain a residue
containing a 20-residue sugar-added peptide having a protecting
group, Boc-Ser(tBu)-Thr(GalNAc)-Ala-Pro-Pro-Ala-H
is(Trt)-Gly-Val-Thr(tBu)-Ser(tBu)-Ala-Pro-Asp(OtBu)-Thr(tBu)-Arg(Pbf)-Pro-
-Ala-Pro-Gly (SEQ ID NO: 27).
[0285] 75 mg (35 .mu.mol) of the obtained 20-residue sugar-added
peptide having a protecting group (SEQ ID NO: 27) and 123 .mu.l
(105 .mu.mol) of benzylmercaptan were added to 3.5 ml of DMF, and
the obtained mixture was then stirred in an argon atmosphere at
-20.degree. C. for 1 hour. Thereafter, PyBOP (91 mg, 175 .mu.mol)
and DIPEA (30 .mu.l, 175 .mu.mol) were added to the reaction
solution, and the obtained mixture was then stirred for 2.5 hours.
Thereafter, diethyl ether was added to the reaction solution to
precipitate crystals, followed by filtration.
TFA/water/TIPS=95/2.5/2.5 was added to the obtained residue to such
an extent that the resin was sufficiently immersed therein, and the
obtained mixture was then stirred at 25.degree. C. for 2 hours. The
resin was removed by filtration, and the filtrate was then
concentrated, so that the obtained residue was recovered. The
obtained residue was purified by HPLC (Vydac column C8,
250.times.10 mm; developing solvents A: 0.1% TFA aqueous solution;
and B: 0.09% TFA; acetonitrile:water=90:10; gradients
A:B=90:10.fwdarw.60:40; 30 minutes; flow rate: 4.0 ml/min) to
obtain a 20-residue sugar-added peptide having benzylthioester at
the C-terminus thereof,
Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-Asp--
Thr-Arg-Pro-Ala-Pro-Gly-SBn (SEQ ID NO: 28).
[0286] On the other hand, 0.02 mmol out of the previously obtained
19-residue sugar-added peptide having a protecting group (SEQ ID
NO: 26) on the solid-phase resin was transferred into another
solid-phase synthesis column. Boc-Thia (0.04 mmol), DIPCl (0.1
mmol), and HOBt (0.1 mmol) that had been dissolved in 1.5 mL of DMF
and had been activated for 15 minutes were added to the column, and
a reaction was then carried out for 20 minutes. Thereafter, the
reaction solution was filtrated, and a reagent
(TFA/water/TIPS=95/2.5/2.5) was added thereto to such an extent
that the resin was sufficiently immersed therein. The obtained
mixture was stirred at 25.degree. C. for 2 hours. The resin was
removed by filtration, and the filtrate was then concentrated. The
obtained residue was dissolved in 2.1 ml of 0.2 M methoxyamine and
0.1 M sodium phosphate buffer (pH 4; containing 6 M guanidine
hydrochloride), so that the thiazoline at the N-terminus was
subjected to ring-opening. Thereafter, the reaction solution was
purified by HPLC (Vydac column C8, 250.times.10 mm; developing
solvents A: 0.1% TFA aqueous solution; and B: 0.08% TFA;
acetonitrile:water=90:10; gradients A:B=90:10.fwdarw.60:40; 30
minutes; flow rate: 4.0 ml/min) to obtain a 20-residue sugar-added
peptide,
Cys-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-Asp-Thr-Arg-P-
ro-Ala-Pro-Gly (SEQ ID NO: 29).
[0287] 13 mg of the thus obtained 20-residue sugar-added peptide
(SEQ ID NO: 29) and 12 mg of the previously obtained 20-residue
peptide having benzylthioester at the C-terminus thereof (SEQ ID
NO: 28) were dissolved in 2.9 mL of a phosphate buffer (pH 7.2;
containing 6 M guanidine hydrochloride). Thereafter, 30 mg of
4-mercaptophenylacetic acid and 17 mg of
tris(2-carboxyethyl)phosphine were added to the above solution, and
a reaction was then carried out for 3 hours. The reaction solution
was purified by HPLC (Vydac column C8, 250.times.10 mm; developing
solvents A: 0.1% TFA aqueous solution; and B: 0.09% TFA;
acetonitrile:water=90:10; gradients A:B=90:10.fwdarw.60:40; 30
minutes; flow rate: 4.0 ml/min) to obtain 18 mg of a 40-residue
sugar-added peptide,
Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-Asp-Thr-Arg-P-
ro-Ala-Pro-Gly-Cys-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-
-Asp-Thr-Arg-Pro-Ala-Pro-Gly (SEQ ID NO: 30).
[0288] 16 mg of the obtained 40-residue sugar-added peptide (SEQ ID
NO: 30) was placed in a round-bottom flask, and it was dissolved in
3.8 mL of a 0.25 M Tris-HCl buffer (pH 8.6; containing 6 M
guanidine hydrochloride solution and 3.3 mM EDTA). Thereafter,
2-mercaptoethanol (3 .mu.L) was added to the obtained solution, and
the obtained mixture was then stirred for 10 minutes. Subsequently,
1.27 mL of acetonitrile containing 25 mg of
methyl-4-nitrobenzenesulfonate was added to the reaction solution.
Twenty-five minutes later, 0.45 mL of a 10% TFA solution was added
to the reaction solution for neutralization, and 2 mL of diethyl
ether was then added thereto, followed by separation and washing 3
times. After a water layer had been concentrated under a reduced
pressure, the obtained residue was purified by HPLC (Vydac column
C-4, 250.times.4.6 mm; developing solvents A: 0.1% TFA aqueous
solution; and B: 0.08% TFA; acetonitrile:water=90:10; gradients
A:B=100:0.fwdarw.100:0.fwdarw.90:10.fwdarw.60:40; 0
minute.fwdarw.10 minutes.fwdarw.40 minutes; flow rate: 1.2 ml/min)
to obtain 13 mg of a mixture comprising a 40-residue sugar-added
peptide,
Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-Asp-Thr-Arg-P-
ro-Ala-Pro-Gly-Cys(Me)-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-
-Pro-Asp-Thr-Arg-Pro-Ala-Pro-Gly (SEQ ID NO: 31), wherein the
sulfur atom of the cysteine residue at position 21 was
methylated.
[0289] 12 mg of the obtained mixture comprising the 40-residue
sugar-added peptide (SEQ ID NO: 31), wherein the sulfur atom of the
cysteine residue at position 21 had been methylated, was placed in
a round-bottom flask, and it was dissolved in 2.9 mL of a 80%
formic acid aqueous solution. Thereafter, 152 mg of cyanogen
bromide was added to the solution at 25.degree. C. The reactor was
shaded from light, and a reaction was then carried out at
37.degree. C. Thirty-six hours later, the reaction solution was
concentrated under a reduced pressure. The residue was dissolved in
2.9 mL of trifluoroacetic acid, and thereafter, 2.4 mg of ammonium
iodide and 24 .mu.L of dimethyl sulfide were added thereto,
followed by a reaction at a room temperature for 30 minutes. Thirty
minutes later, 6 mL of water was added to the reaction system, and
separation and washing were then carried out using carbon
tetrachloride. A water layer was concentrated under a reduced
pressure, and the residue was then dissolved in 1.4 mL of a 5%
hydrazine aqueous solution, followed by a reaction at a room
temperature for 10 minutes. Ten minutes later, 0.14 mL of acetic
acid was added to the reaction solution, and the obtained mixture
was then purified by HPLC (Vydac column C-4, 250.times.4.6 mm;
developing solvents A: 50 mM AcONH4 aqueous solution; and B:
acetonitrile; gradients A:B=90:10.fwdarw.70:30; 60 minutes; flow
rate: 1.0 ml/min) to obtain 2.3 mg of a 40-residue sugar-added
peptide of interest,
Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-Ser-Ala-Pro-Asp-
-Thr-Arg-Pro-Ala-Pro-Gly-Ser-Thr(GalNAc)-Ala-Pro-Pro-Ala-His-Gly-Val-Thr-S-
er-Ala-Pro-Asp-Thr-Arg-Pro-Ala-Pro-Gly (SEQ ID NO: 32).
[0290] ESI-MS: Calcd for: [M+3H].sup.3+1388.5, found, 1388.6
Example 7
Production of Glu-Ser-Asn-Gly-Thr-Leu-Thr-Leu
[0291] Trt chloride resin (manufactured by Merck) (100 .mu.mol) was
placed in a solid-phase synthesis column, and it was then fully
washed with methylene chloride (DCM) and DMF. Thereafter, the
resultant was fully swollen with DCM. After filtration, Fmoc-Gly
(0.20 mmol) and DIPEA (0.4 mmol) were dissolved in DCM (0.66 mL),
and the obtained solution was then placed in the solid-phase
synthesis column, followed by stirring at 25.degree. C. for 2
hours. After completion of the stirring operation, the resin was
sufficiently washed with DCM:MeOH:DIPEA=17:2:1, DCM, and DMF, and
the Fmoc group was treated with a 20% piperidine/DMF solution (1
mL) for 20 minutes for deprotection. After washing with DMF, as for
the subsequent elongation of a peptide chain, amino acids were
successively condensed according to the below-mentioned method.
[0292] An amino acid whose amino group had been protected, HOBt
(67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 63.1 mg, 0.50 mmol)
were dissolved in DMF (2 mL), and the obtained solution was then
activated for 15 minutes. Thereafter, the solution was placed in
the solid-phase synthesis column. After stirring at 25.degree. C.
for 1 hour, the Fmoc group was treated with a 20% piperidine/DMF
solution (2 mL) for 20 minutes for deprotection. These operations
were repeated, so that amino acids were successively condensed. As
the protected amino acids, Fmoc-Asn (177.2 mg, 0.50 mmol),
Fmoc-Ser(tBu) (191.6 mg, 0.50 mmol), and Boc-Glu(OtBu) (151.6 mg,
0.50 mmol) were used. On the solid-phase resin, a 4-residue peptide
having a protecting group, Boc-Glu(OtBu)-Ser(tBu)-Asn-Gly (SEQ ID
NO: 33), was obtained.
[0293] After the resultant had been washed with DMF and DCM, 2 mL
of AcOH:MeOH:DCM=5:4:1 was added thereto, followed by a reaction at
a room temperature for 2 hours. Two hours later, the reaction
solution was recovered by filtration, and it was then concentrated
under a reduced pressure to obtain a residue. 1 mL of benzene was
added to this residue, an azeotropic operation was then carried out
thereon 2 times, and the resultant was then dissolved in 0.81 mL of
DMF. To the obtained solution, 42.1 mg of PyBOP, 14 .mu.l of DIPEA,
and 57 .mu.l of benzylmercaptan were added in an argon atmosphere
at 0.degree. C., and the obtained mixture was then stirred for 30
minutes. After completion of the reaction, the reaction solution
was neutralized with a saturated ammonium chloride aqueous
solution, and it was then separated and washed with water and a
saturated saline. An organic layer was dried over magnesium
sulfate, and it was then filtrated. Thereafter, the filtrate was
concentrated under a reduced pressure. The obtained residue was
purified by silica gel column chromatography (developing solvents;
ethyl acetate:MeOH:water=20:2:1) to gather fractions containing
peptides of interest, and the gathered fractions were then
concentrated under a reduced pressure. The obtained residue was
dissolved in a 95% TFA aqueous solution, and a reaction was then
carried out for 10 minutes. The reaction solution was concentrated
under a reduced pressure to obtain a 4-residue peptide having
benzylthioester at the C-terminus thereof, Glu-Ser-Asn-Gly-SBn (SEQ
ID NO: 34).
[0294] On the other hand, Wang resin (manufactured by Merck) (0.1
mmol) was placed in a solid-phase synthesis column, and it was then
fully washed with methylene chloride (DCM) and DMF. Thereafter, the
resultant was fully swollen with DCM. Fmoc-Leu (0.50 mmol), MSNT
(0.50 mmol), and N-methylimidazole (0.375 mmol) were dissolved in
DCM (2 mL), and the obtained solution was then placed in the
solid-phase synthesis column, followed by stirring at 25.degree. C.
for 2 hours. Two hours later, the resin was fully washed with DCM
and DMF, and the Fmoc group was treated with a 20% piperidine/DMF
solution (2 mL) for 20 minutes for deprotection. After washing with
DMF and DCM, as for the subsequent elongation of a peptide chain,
amino acids were successively condensed according to the
below-mentioned method.
[0295] An amino acid whose amino group had been protected, HOBt
(67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 0.50 mmol) were
dissolved in 2 mL of DMF, and the obtained solution was then
activated for 15 minutes. Thereafter, the solution was placed in
the solid-phase synthesis column. After stirring at 25.degree. C.
for 1 hour, the Fmoc group was treated with a 20% piperidine/DMF
solution (2 mL) for 20 minutes for deprotection. These operations
were repeated, so that amino acids were successively condensed. As
the protected amino acids, Fmoc-Thr(tBu) (198.8 mg, 0.50 mmol) and
Fmoc-Leu (176.8 mg, 0.50 mmol) were used, and a 3-residue peptide
having a protecting group, Fmoc-Leu-Thr(tBu)-Leu, was obtained on
the solid-phase resin. Thereafter, the Fmoc group was treated with
a 20% piperidine/DMF solution (2 mL) for 20 minutes for
deprotection. Then, a threonine derivative having a protecting
group, Boc-Thr(S-SsecBu) synthesized in Synthesis Example 1
separately, HOBt (67.6 mg, 0.50 mmol), and DIPCl (77.0 .mu.L, 0.50
mmol) that had been dissolved in 2 mL of DMF and had been then
activated for 15 minutes were added to the solution. The obtained
mixture was then reacted for 3 hours to obtain a 4-residue peptide
having a protecting group, Boc-Thr(S-SsecBu)-Leu-Thr(tBu)-Leu (SEQ
ID NO: 35), on the solid-phase resin. A 95% TFA aqueous solution
was added to the aforementioned 4-residue peptide, and a reaction
was then carried out at a room temperature for 2 hours. Thereafter,
the resin was removed by filtration, and the filtrate was then
concentrated under a reduced pressure to obtain a 4-residue peptide
having a protecting group, Thr(S-SsecBu)-Leu-Thr-Leu (SEQ ID NO:
36).
[0296] 2.2 mg of the thus obtained 4-residue peptide having a
protecting group (SEQ ID NO: 36) and 2.2 mg of the previously
obtained 4-residue peptide having thioester (SEQ ID NO: 34) were
dissolved in xx mL of a 0.2 M sodium phosphate buffer (pH 7.3;
containing 6 M guanidine hydrochloride and 20 mg of
4-mercaptophenylacetic acid), followed by a reaction for 3.5 hours.
After completion of the reaction, dithiothreitol was added to the
reaction solution, and the obtained mixture was then stirred for 10
minutes. Thereafter, the reaction solution was purified by HPLC
(Cadenza column C18, 75.times.4.6 mm; developing solvents A: 0.1%
TFA aqueous solution; and B: 0.1% TFA; acetonitrile:water=90:10;
gradients A:B=90:10.fwdarw.35:65; 15 minutes; flow rate: 1.0
ml/min) to obtain a 8-residue peptide,
Glu-Ser-Asn-Gly-Thr(SH)-Leu-Thr-Leu (SEQ ID NO: 37), wherein the
side chain hydroxyl group of the threonine residue at position 5
was substituted with a thiol group. The threonine derivative
residue contained in the obtained peptide was considered to have a
configuration mainly represented by the following formula:
##STR00016##
[0297] 3.5 mg of the obtained 8-residue peptide (SEQ ID NO: 37),
wherein the side chain hydroxyl group of the threonine residue at
position 5 had been substituted with a thiol group, was placed in a
round-bottom flask, and it was dissolved in 4.1 mL of a 0.25 M
Tris-HCl buffer (pH 8.6; containing 6 M guanidine hydrochloride
solution and 3.3 mM EDTA). Thereafter, 2-mercaptoethanol (2.9
.mu.L) was added to the obtained solution, and the obtained mixture
was then stirred for 15 minutes. Subsequently, 1.37 mL of
acetonitrile, in which methyl-4-nitrobenzenesulfonate (26.2 mg) had
been dissolved, was added to the reaction solution, and a reaction
was then carried out for 50 minutes. After completion of the
reaction, a 10% TFA solution (1.0 mL) was added to the reaction
solution for neutralization, and the reaction solution was then
purified by HPLC (Cadenza column C18, 75.times.4.6 mm; developing
solvents A: 0.1% TFA aqueous solution; and B: 0.1% TFA;
acetonitrile:water=90:10; gradients A:B=90:10.fwdarw.35:65; 15
minutes; flow rate: 1.0 ml/min) to obtain a 8-residue peptide,
Glu-Ser-Asn-Gly-Thr(SMe)-Leu-Thr-Leu (SEQ ID NO: 38), wherein the
sulfur atom of the substituted thiol group of the threonine residue
at position 5 was methylated.
[0298] 4 mg of the obtained 8-residue peptide (SEQ ID NO: 38),
wherein the sulfur atom of the substituted thiol group of the
threonine residue at position 5 had been methylated, was placed in
a round-bottom flask, and it was dissolved in 4.63 mL of a 70%
formic acid aqueous solution. Thereafter, 49.0 mg of cyanogen
bromide was added to the solution, and the obtained mixture was
then stirred under shaded conditions in an argon-substituted
atmosphere at 37.degree. C. Forty-eight hours later, the reaction
solution was concentrated under a reduced pressure, and 0.93 mL of
a 5% hydrazine aqueous solution was then added thereto, followed by
a reaction for 15 minutes. 60 .mu.L of acetic acid was added to the
reaction solution, and the reaction solution was then purified by
HPLC (Cadenza column C18, 75.times.4.6 mm; developing solvents A:
0.1% TFA aqueous solution; and B: 0.1% TFA;
acetonitrile:water=90:10; gradients A:B=85:15.fwdarw.45:55; 15
minutes; flow rate: 1.0 ml/min) to obtain a 8-residue peptide of
interest, Glu-Ser-Asn-Gly-Thr-Leu-Thr-Leu (SEQ ID NO: 39). It was
assumed from the reaction mechanism that the configuration of the
threonine residues contained in the obtained peptide would be the
same as that of a natural one. ESI-MS: Calcd for
C.sub.34H.sub.59N.sub.9O.sub.15: [M+1H].sup.1+ 834.41, found,
835.1
Synthesis Example 1
Synthesis of Boc-Thr(S-SsecBu)
[0299] Boc-Thr (300 mg, 1.37 mmol) was dissolved in distilled DMF
(6.85 mL, 200 mM), and
N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (786
mg, 4.1 mmol) and HOBt (370 mg, 2.74 mmol) were then added to the
solution. The obtained mixture was stirred at 0.degree. C. for 15
minutes. Thereafter, TMSEtOH (1.95 mL, 13.7 mmol) was added to the
reaction solution, and the obtained mixture was then stirred at
0.degree. C. Twenty hours later, after confirming the termination
of the reaction by TLC, the reaction solution was diluted with
ethyl acetate, and it was then neutralized with a saturated sodium
bicarbonate solution. The solution was then separated and washed
with water twice, and with a saturated saline once. An organic
layer was dried over magnesium sulfate, and was then filtrated. The
filtrate was concentrated under a reduced pressure. The residue was
purified by silica gel column chromatography (developing solvent;
ethyl acetate:hexane=1:3) to obtain a Boc-Thr-TMS ester (yield: 342
mg, yield constant: 78%).
[0300] The obtained Boc-Thr-TMS ester was subjected to azeotropy
with benzene 2 times, and the residue was then dissolved in 20.8 mL
of DCM. 322 mL (4.16 mmol) of methanesulfonyl chloride and 1.16 mL
(8.32 mmol) of triethylamine were added to the solution, and the
obtained mixture was then stirred at 0.degree. C. Ten minutes
later, after confirming the termination of the reaction by TLC, the
reaction solution was diluted with DCM, and it was then neutralized
with a saturated ammonium chloride aqueous solution. Thereafter,
the solution was separated and washed with water twice, and with a
saturated saline once. An organic layer was dried over magnesium
sulfate, and was then filtrated. The filtrate was concentrated
under a reduced pressure. The residue was purified by silica gel
column chromatography (developing solvent; ethyl
acetate:hexane=1:4) to obtain a Boc-Thr(OMs)-TMS ester (yield
amount: 724.6 mg, yield percentage: 88%).
[0301] 1.06 g of the obtained Boc-Thr(OMs)-TMS ester was subjected
to azeotropy with benzene 2 times, and it was then dried with a
desiccator overnight. The resultant was dissolved in 8.9 mL of DMF.
Thereafter, a solution obtained by dissolving 0.57 mL (7.95 mmol)
of thioacetic acid and 0.79 mL (5.3 mmol) of DBU in 4.4 mL of DMF
followed by a reaction for 20 minutes was added to the above
solution. The obtained mixture was stirred at 45.degree. C.
Eighteen hours later, after confirming the termination of the
reaction by TLC, the reaction solution was neutralized with a
saturated ammonium chloride aqueous solution, and it was then
separated and washed with water twice, and with a saturated saline
once. An organic layer was dried over magnesium sulfate, and was
then filtrated. The filtrate was concentrated under a reduced
pressure. The residue was purified by silica gel column
chromatography (developing solvent; ethyl acetate:hexane=1:20) to
obtain a Boc-Thr(SAc)-TMS ester (yield amount: 536.8 mg, yield
percentage: 53.7%).
[0302] 1.1 g of the obtained Boc-Thr(SAc)-TMS ester was dissolved
in 100 mL of methanol, and thereafter, a solution obtained by
dissolving dipyridyl disulfide (3.21 g, 14.6 mmol) and 1.75 mL
(16.1 mmol) of Sec-BuSH in 5.5 mL of methanol was added to the
above solution. Thereafter, 11.7 mL of a methanol solution of
sodium hydroxide (500 mM) was added thereto. Eighteen hours later,
after confirming the termination of the reaction by TLC, the
reaction solution was neutralized with a 1% acetic acid aqueous
solution, it was then concentrated under a reduced pressure, and it
was then diluted with ethyl acetate. The resultant was separated
and washed with water twice, and then with a saturated saline once.
An organic layer was dried over magnesium sulfate, and it was then
filtrated. The filtrate was concentrated under a reduced pressure.
The residue was purified by silica gel column chromatography
(developing solvent; ethyl acetate:hexane=1:40). The obtained
mixture was subjected to azeotropy with benzene 2 times, and it was
then dissolved in 29 mL (100 mM) of DMF. 2.28 mg (7.3 mmol) of
tetrabutylammonium fluoride trihydrate was added to the above
solution, and the obtained mixture was stirred at 0.degree. C.
After stirring for 15 minutes, after confirming the termination of
the reaction by TLC, the reaction solution was neutralized with a
saturated ammonium chloride aqueous solution. This reaction
solution was washed with water twice, and then with a saturated
saline once. An organic layer was dried over magnesium sulfate, and
was then filtrated. The filtrate was concentrated under a reduced
pressure. The residue was purified by silica gel column
chromatography (developing solvent; ethyl
acetate:hexane=1:4.fwdarw.ethyl acetate:methanol:water=20:2:1) to
obtain Boc-Thr(S-SsecBu) of interest (yield amount: 650 mg, yield
percentage: 69%).
Sequence Listing Free Text
[0303] SEQ ID NO: 1 is an amino acid sequence having a protecting
group of Example 1.
[0304] SEQ ID NO: 2 is an acetylated amino acid sequence of Example
1.
[0305] SEQ ID NO: 3 is an amino acid sequence having methylated
cysteine of Example 1.
[0306] SEQ ID NO: 4 is an acetylated amino acid sequence of Example
1.
[0307] SEQ ID NO: 5 is an amino acid sequence having a protecting
group of Example 2.
[0308] SEQ ID NO: 6 is an amino acid sequence of Example 2.
[0309] SEQ ID NO: 7 is an amino acid sequence having methylated
cysteine of Example 2.
[0310] SEQ ID NO: 8 is an amino acid sequence of Example 2.
[0311] SEQ ID NO: 9 is an amino acid sequence having a protecting
group of Example 3.
[0312] SEQ ID NO: 10 is an amino acid sequence of Example 3.
[0313] SEQ ID NO: 11 is an amino acid sequence having methylated
cysteine of Example 3.
[0314] SEQ ID NO: 12 is an amino acid sequence of Example 3.
[0315] SEQ ID NO: 13 is a sugar chain-added amino acid sequence
having a protecting group of Example 4.
[0316] SEQ ID NO: 14 is a sugar chain-added amino acid sequence
having a protecting group of Example 4.
[0317] SEQ ID NO: 15 is a sugar chain-added amino acid sequence
having a benzylthioester group of Example 4.
[0318] SEQ ID NO: 16 is an amino acid sequence having a protecting
group of Example 4.
[0319] SEQ ID NO: 17 is an amino acid sequence of Example 4.
[0320] SEQ ID NO: 18 is a sugar chain-added amino acid sequence of
Example 4.
[0321] SEQ ID NO: 19 is a sugar chain-added amino acid sequence
having methylated cysteine of Example 4.
[0322] SEQ ID NO: 20 is a sugar chain-added amino acid sequence of
Example 4.
[0323] SEQ ID NO: 21 is an amino acid sequence having a protecting
group and methionine sulfoxide of Example 5.
[0324] SEQ ID NO: 22 is an acetylated amino acid sequence having
methionine sulfoxide of Example 5.
[0325] SEQ ID NO: 23 is an acetylated amino acid sequence having
methylated cysteine and methionine sulfoxide of Example 5.
[0326] SEQ ID NO: 24 is an acetylated amino acid sequence of
Example 5.
[0327] SEQ ID NO: 25 is an amino acid sequence having a protecting
group of Example 6.
[0328] SEQ ID NO: 26 is a sugar chain-added amino acid sequence
having a protecting group of Example 6.
[0329] SEQ ID NO: 27 is a sugar chain-added amino acid sequence
having a protecting group of Example 6.
[0330] SEQ ID NO: 28 is a sugar chain-added amino acid sequence
having a benzylthioester group of Example 6.
[0331] SEQ ID NO: 29 is a sugar chain-added amino acid sequence of
Example 6.
[0332] SEQ ID NO: 30 is a sugar chain-added amino acid sequence of
Example 6.
[0333] SEQ ID NO: 31 is a sugar chain-added amino acid sequence
having methylated cysteine of Example 6.
[0334] SEQ ID NO: 32 is a sugar chain-added amino acid sequence of
Example 6.
[0335] SEQ ID NO: 33 is an amino acid sequence having a protecting
group of Example 7.
[0336] SEQ ID NO: 34 is an amino acid sequence having a
benzylthioester group of Example 7.
[0337] SEQ ID NO: 35 is an amino acid sequence having a protecting
group and a threonine derivative of Example 7.
[0338] SEQ ID NO: 36 is an amino acid sequence having a threonine
derivative of Example 7.
[0339] SEQ ID NO: 37 is an amino acid sequence having a threonine
derivative of Example 7.
[0340] SEQ ID NO: 38 is an amino acid sequence having a threonine
derivative of Example 7.
[0341] SEQ ID NO: 39 is an amino acid sequence of Example 7.
INDUSTRIAL APPLICABILITY
[0342] The present invention provides a method for producing a
peptide and a glycopeptide. According to the present invention,
even in a case in which a peptide that does not contain cysteine is
to be obtained, a ligation method can be applied. In addition, the
present invention is also useful for the production of an N-linked
glycopeptide and an O-linked glycopeptide.
Sequence CWU 1
1
3914PRTartificial sequencesynthetic construct 1Ala Cys Gly
Leu124PRTartificial sequencesynthetic construct 2Ala Cys Gly
Leu134PRTartificial sequencesynthetic construct 3Ala Cys Gly
Leu144PRTartificial sequencesynthetic construct 4Ala Ser Gly
Leu158PRTartificial sequencesynthetic construct 5Val Asp Lys Ala
Val Cys Gly Leu1 568PRTartificial sequencesynthetic construct 6Val
Asp Lys Ala Val Cys Gly Leu1 5 78PRTartificial sequencesynthetic
construct 7Val Asp Lys Ala Val Cys Gly Leu1 5 88PRTartificial
sequencesynthetic construct 8Val Asp Lys Ala Val Ser Gly Leu1 5
911PRTartificial sequencesynthetic construct 9Leu Phe Arg Val Tyr
Cys Asn Phe Leu Arg Gly1 5 101011PRTartificial sequencesynthetic
construct 10Leu Phe Arg Val Tyr Cys Asn Phe Leu Arg Gly1 5
101111PRTartificial sequencesynthetic construct 11Leu Phe Arg Val
Tyr Cys Asn Phe Leu Arg Gly1 5 101211PRTartificial
sequencesynthetic construct 12Leu Phe Arg Val Tyr Ser Asn Phe Leu
Arg Gly1 5 10136PRTartificial sequencesynthetic construct 13Ala Leu
Leu Val Asn Ser1 5 146PRTartificial sequencesynthetic construct
14Ala Leu Leu Val Asn Ser1 5 156PRTartificial sequencesynthetic
construct 15Ala Leu Leu Val Asn Ser1 5 1614PRTartificial
sequencesynthetic construct 16Cys Gln Pro Trp Glu Pro Leu Gln Leu
His Val Asp Lys Ala1 5 101714PRTartificial sequencesynthetic
construct 17Cys Gln Pro Trp Glu Pro Leu Gln Leu His Val Asp Lys
Ala1 5 101820PRTartificial sequencesynthetic construct 18Ala Leu
Leu Val Asn Ser Cys Gln Pro Trp Glu Pro Leu Gln Leu His 1 5 10 15
Val Asp Lys Ala 201920PRTartificial sequencesynthetic construct
19Ala Leu Leu Val Asn Ser Cys Gln Pro Trp Glu Pro Leu Gln Leu His 1
5 10 15 Val Asp Lys Ala 202020PRTartificial sequencesynthetic
construct 20Ala Leu Leu Val Asn Ser Ser Gln Pro Trp Glu Pro Leu Gln
Leu His 1 5 10 15 Val Asp Lys Ala 20215PRTartificial
sequencesynthetic construct 21Gly Cys Gly Met Ala1 5
225PRTartificial sequencesynthetic construct 22Gly Cys Gly Met Ala1
5 235PRTartificial sequencesynthetic construct 23Gly Cys Gly Met
Ala1 5 245PRTartificial sequencesynthetic construct 24Gly Ser Gly
Met Ala1 5 2518PRTartificial sequencesynthetic construct 25Ala Pro
Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala 1 5 10
15Pro Gly2619PRTartificial sequencesynthetic construct 26Thr Ala
Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro 1 5 10
15Ala Pro Gly2720PRTartificial sequencesynthetic construct 27Ser
Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 1 5 10
15Pro Ala Pro Gly 202820PRTartificial sequencesynthetic construct
28Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 1
5 10 15Pro Ala Pro Gly 202920PRTartificial sequencesynthetic
construct 29Cys Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp
Thr Arg 1 5 10 15Pro Ala Pro Gly 203040PRTartificial
sequencesynthetic construct 30Ser Thr Ala Pro Pro Ala His Gly Val
Thr Ser Ala Pro Asp Thr Arg 1 5 10 15Pro Ala Pro Gly Cys Thr Ala
Pro Pro Ala His Gly Val Thr Ser Ala 20 25 30Pro Asp Thr Arg Pro Ala
Pro Gly 35 403140PRTartificial sequencesynthetic construct 31Ser
Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 1 5 10
15Pro Ala Pro Gly Cys Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala
20 25 30Pro Asp Thr Arg Pro Ala Pro Gly 35 403240PRTartificial
sequencesynthetic construct 32Ser Thr Ala Pro Pro Ala His Gly Val
Thr Ser Ala Pro Asp Thr Arg 1 5 10 15Pro Ala Pro Gly Ser Thr Ala
Pro Pro Ala His Gly Val Thr Ser Ala 20 25 30Pro Asp Thr Arg Pro Ala
Pro Gly 35 40334PRTartificial sequencesynthetic construct 33Glu Ser
Asn Gly 1 344PRTartificial sequencesynthetic construct 34Glu Ser
Asn Gly 1 354PRTartificial sequencesynthetic construct 35Xaa Leu
Thr Leu 1 364PRTartificial sequencesynthetic construct 36Xaa Leu
Thr Leu 1 378PRTartificial sequencesynthetic construct 37Glu Ser
Asn Gly Xaa Leu Thr Leu 1 5 388PRTartificial sequencesynthetic
construct 38Glu Ser Asn Gly Xaa Leu Thr Leu 1 5 398PRTartificial
sequencesynthetic construct 39Glu Ser Asn Gly Thr Leu Thr Leu 1
5
* * * * *