U.S. patent application number 13/815721 was filed with the patent office on 2014-05-22 for single-chain multivalent binding proteins with effector function. This patent application is currently assigned to EMERGENT PRODUCT DEVELOPMENT SEATTLE, LLC. The applicant listed for this patent is ROBERT BADER, WILLIAM BRADY, LAURA SUE GROSMAIRE, MARTHA SUSAN HAYDEN-LEDBETTER, JEFFREY A. LEDBETTER, PETER ARMSTRONG THOMPSON. Invention is credited to ROBERT BADER, WILLIAM BRADY, LAURA SUE GROSMAIRE, MARTHA SUSAN HAYDEN-LEDBETTER, JEFFREY A. LEDBETTER, PETER ARMSTRONG THOMPSON.
Application Number | 20140141022 13/815721 |
Document ID | / |
Family ID | 38832805 |
Filed Date | 2014-05-22 |
United States Patent Application | 20140141022 |
Kind Code | A1 |
THOMPSON; PETER ARMSTRONG ; et al. | May 22, 2014 |
Multivalent binding peptides, including bi-specific binding peptides, having immunoglobulin effector function are provided, along with encoding nucleic acids, vectors and host cells as well as methods for making such peptides and methods for using such peptides to treat or prevent a variety of diseases, disorders or conditions, as well as to ameliorate at least one symptom associated with such a disease, disorder or condition.
Inventors: | THOMPSON; PETER ARMSTRONG; (BELLEVUE, WA) ; LEDBETTER; JEFFREY A.; (SHORELINE, WA) ; HAYDEN-LEDBETTER; MARTHA SUSAN; (SHORELINE, WA) ; GROSMAIRE; LAURA SUE; (HOBART, WA) ; BADER; ROBERT; (SEATTLE, WA) ; BRADY; WILLIAM; (BOTHELL, WA) | ||||||||||
Applicant: |
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Assignee: | EMERGENT PRODUCT DEVELOPMENT
SEATTLE, LLC SEATTLE WA |
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Family ID: | 38832805 | ||||||||||
Appl. No.: | 13/815721 | ||||||||||
Filed: | March 15, 2013 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
12304562 | Jul 22, 2010 | 8409577 | ||
PCT/US2007/071052 | Jun 12, 2007 | |||
13815721 | ||||
60813261 | Jun 12, 2006 | |||
60853287 | Oct 20, 2006 | |||
Current U.S. Class: | 424/178.1 ; 435/252.3; 435/252.31; 435/252.33; 435/252.35; 435/254.21; 435/320.1; 435/326; 530/391.1; 536/23.53 |
Current CPC Class: | A61P 35/00 20180101; C07K 2317/31 20130101; C07K 2319/30 20130101; A61P 25/04 20180101; A61P 31/00 20180101; C07K 2317/53 20130101; A61P 17/06 20180101; C07K 16/2818 20130101; C07K 16/2851 20130101; C07K 16/2887 20130101; C07K 16/2875 20130101; C07K 16/46 20130101; A61P 31/22 20180101; C07K 2317/52 20130101; C07K 2317/732 20130101; C07K 2317/622 20130101; A61P 37/00 20180101; C07K 16/468 20130101; A61P 19/02 20180101; A61P 37/02 20180101; C07K 16/28 20130101; A61K 2039/507 20130101; C07K 16/18 20130101; C07K 2317/734 20130101; C07K 16/2833 20130101; A61P 31/12 20180101; C07K 16/2809 20130101; A61K 2039/505 20130101; C07K 16/2896 20130101; A61P 25/00 20180101; C07K 16/30 20130101; C07K 2317/34 20130101; C07K 2317/64 20130101; C07K 16/2803 20130101; C07K 16/2827 20130101; A61P 3/10 20180101; A61P 11/06 20180101; A61P 1/04 20180101; A61P 37/06 20180101; A61P 43/00 20180101; C07K 2317/24 20130101; C07K 16/2878 20130101; A61P 29/00 20180101 |
Class at Publication: | 424/178.1 ; 530/391.1; 536/23.53; 435/320.1; 435/326; 435/254.21; 435/252.33; 435/252.31; 435/252.3; 435/252.35 |
International Class: | C07K 16/18 20060101 C07K016/18 |
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 379 <210> SEQ ID NO 1 <211> LENGTH: 795
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - anti-CD20 (2H7) LH (DNA) <400> SEQUENCE: 1
atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc
60 agaggacaaa ttgttctctc ccagtctcca gcaatcctgt ctgcatctcc
aggggagaag 120 gtcacaatga cttgcagggc cagctcaagt gtaagttaca
tgcactggta ccagcagaag 180 ccaggatcct cccccaaacc ctggatttat
gccccatcca acctggcttc tggagtccct 240 gctcgcttca gtggcagtgg
gtctgggacc tcttactctc tcacaatcag cagagtggag 300 gctgaagatg
ctgccactta ttactgccag cagtggagtt ttaacccacc cacgttcggt 360
gctgggacca agctggagct gaaagatggc ggtggctcgg gcggtggtgg atctggagga
420 ggtgggagct ctcaggctta tctacagcag tctggggctg agtcggtgag
gcctggggcc 480 tcagtgaaga tgtcctgcaa ggcttctggc tacacattta
ccagttacaa tatgcactgg 540 gtaaagcaga cacctagaca gggcctggaa
tggattggag ctatttatcc aggaaatggt 600 gatacttcct acaatcagaa
gttcaagggc aaggccacac tgactgtaga caaatcctcc 660 agcacagcct
acatgcagct cagcagcctg acatctgaag actctgcggt ctatttctgt 720
gcaagagtgg tgtactatag taactcttac tggtacttcg atgtctgggg cacagggacc
780 acggtcaccg tctct 795 <210> SEQ ID NO 2 <211>
LENGTH: 265 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic polypeptide - anti-CD20 (2H7) LH (AA) <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(22) <223> OTHER INFORMATION: Leader <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <400>
SEQUENCE: 2 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser
Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln
Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr
Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His Trp
Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile Tyr
Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe Ser
Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser Arg
Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110
Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115
120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg
Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser Gly
Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln Thr
Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro Gly
Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys Ala
Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met Gln
Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235
240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp
245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser 260 265 <210>
SEQ ID NO 3 <211> LENGTH: 813 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polynucleotide - anti-CD28
(2e12) LH (DNA) <400> SEQUENCE: 3 atggattttc aagtgcagat
tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60 agaggagtcg
acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 120
agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg
180 cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc
tgctagcaac 240 gtagaatctg gggtccctgc caggtttagt ggcagtgggt
ctgggacaga ctttagcctc 300 aacatccatc ctgtggagga ggatgatatt
gcaatgtatt tctgtcagca aagtaggaag 360 gttccatgga cgttcggtgg
aggcaccaag ctggaaatca aacggggtgg cggtggatcc 420 ggcggaggtg
ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 480
ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc
540 ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg
gctgggaatg 600 atatggggtg atggaagcac agactataat tcagctctca
aatccagact atcgatcacc 660 aaggacaact ccaagagcca agttttctta
aaaatgaaca gtctgcaaac tgatgacaca 720 gccagatact actgtgcccg
agatggttat agtaactttc attactatgt tatggactac 780 tggggtcaag
gaacctcagt caccgtctcc tct 813 <210> SEQ ID NO 4 <211>
LENGTH: 271 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic polypeptide - anti-CD28 (2e12) LH (AA) <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(23) <223> OTHER INFORMATION: Leader <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(24)..(135) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(136)..(150) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(151)..(271) <223> OTHER INFORMATION: VH <400>
SEQUENCE: 4 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser
Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Val Asp Ile Val Leu Thr
Gln Ser Pro Ala 20 25 30 Ser Leu Ala Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala 35 40 45 Ser Glu Ser Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln 50 55 60 Gln Lys Pro Gly Gln Pro
Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 65 70 75 80 Val Glu Ser Gly
Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 85 90 95 Asp Phe
Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met 100 105 110
Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly 115
120 125 Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly
Gly 130 135 140 Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser
Gly Pro Gly 145 150 155 160 Leu Val Ala Pro Ser Gln Ser Leu Ser Ile
Thr Cys Thr Val Ser Gly 165 170 175 Phe Ser Leu Thr Gly Tyr Gly Val
Asn Trp Val Arg Gln Pro Pro Gly 180 185 190 Lys Gly Leu Glu Trp Leu
Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 195 200 205 Tyr Asn Ser Ala
Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 210 215 220 Lys Ser
Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 225 230 235
240 Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr
245 250 255 Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser
Ser 260 265 270 <210> SEQ ID NO 5 <211> LENGTH: 828
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - anti-CD28 (2e12) HL (DNA) <400> SEQUENCE: 5
atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc
60 agaggagtcc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc
ctcacagagc 120 ctgtccatca catgcaccgt ctcagggttc tcattaaccg
gctatggtgt aaactgggtt 180 cgccagcctc caggaaaggg tctggagtgg
ctgggaatga tatggggtga tggaagcaca 240 gactataatt cagctctcaa
atccagacta tcgatcacca aggacaactc caagagccaa 300 gttttcttaa
aaatgaacag tctgcaaact gatgacacag ccagatacta ctgtgcccga 360
gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc
420 accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg
tgggtcgggt 480 ggcggcggat ctgacattgt gctcacccaa tctccagctt
ctttggctgt gtctctaggt 540 cagagagcca ccatctcctg cagagccagt
gaaagtgttg aatattatgt cacaagttta 600 atgcagtggt accaacagaa
accaggacag ccacccaaac tcctcatctc tgctgctagc 660 aacgtagaat
ctggggtccc tgccaggttt agtggcagtg ggtctgggac agactttagc 720
ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca gcaaagtagg
780 aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgt 828
<210> SEQ ID NO 6 <211> LENGTH: 276 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polypeptide - anti-CD28
(2e12) HL (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(23) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (24)..(144) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(164) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (165)..(276) <223> OTHER
INFORMATION: VL <400> SEQUENCE: 6 Met Asp Phe Gln Val Gln Ile
Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg
Gly Val Gln Val Gln Leu Lys Glu Ser Gly Pro 20 25 30 Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser 35 40 45 Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 50 55
60 Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr
65 70 75 80 Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys
Asp Asn 85 90 95 Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu
Gln Thr Asp Asp 100 105 110 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly
Tyr Ser Asn Phe His Tyr 115 120 125 Tyr Val Met Asp Tyr Trp Gly Gln
Gly Thr Ser Val Thr Val Ser Ser 130 135 140 Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 145 150 155 160 Gly Gly Gly
Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 165 170 175 Val
Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 180 185
190 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro
195 200 205 Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val
Glu Ser 210 215 220 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly
Thr Asp Phe Ser 225 230 235 240 Leu Asn Ile His Pro Val Glu Glu Asp
Asp Ile Ala Met Tyr Phe Cys 245 250 255 Gln Gln Ser Arg Lys Val Pro
Trp Thr Phe Gly Gly Gly Thr Lys Leu 260 265 270 Glu Ile Lys Arg 275
<210> SEQ ID NO 7 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - Heavy Chain GSP1
<400> SEQUENCE: 7 aggtgctgga ggggacagtc actgagctgc 30
<210> SEQ ID NO 8 <211> LENGTH: 28 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - Nested Heavy
Chain <400> SEQUENCE: 8 gtcacwgtca ctgrctcagg gaartagc 28
<210> SEQ ID NO 9 <211> LENGTH: 33 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - Light Chain GSP1
<400> SEQUENCE: 9 gggtgctgct catgctgtag gtgctgtctt tgc 33
<210> SEQ ID NO 10 <211> LENGTH: 32 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - Nested Light
Chain <400> SEQUENCE: 10 caagaagcac acgactgagg cacctccaga tg
32 <210> SEQ ID NO 11 <211> LENGTH: 36 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - Race
Abridged Anchor Primer <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (24)..(25) <223> OTHER
INFORMATION: n is a, c, g, or t <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (29)..(30) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (34)..(35)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 11 ggccacgcgt cgactagtac gggnngggnn gggnng 36 <210>
SEQ ID NO 12 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - T7 primer
<400> SEQUENCE: 12 gtaatacgac tcactatagg 20 <210> SEQ
ID NO 13 <211> LENGTH: 18 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - M13 reverse primer
<400> SEQUENCE: 13 caggaaacag ctatgacc 18 <210> SEQ ID
NO 14 <211> LENGTH: 15 <212> TYPE: PRT <213>
ORGANISM: Homo sapiens <220> FEATURE: <223> OTHER
INFORMATION: human IgG1 hinge domain <400> SEQUENCE: 14 Glu
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 15 <211> LENGTH: 46 <212> TYPE:
DNA <213> ORGANISM: Homo sapiens <220> FEATURE:
<223> OTHER INFORMATION: hVK3L-F3H3 <400> SEQUENCE: 15
gcgataaagc ttgccgccat ggaagcacca gcgcagcttc tcttcc 46 <210>
SEQ ID NO 16 <211> LENGTH: 50 <212> TYPE: DNA
<213> ORGANISM: Homo sapiens <220> FEATURE: <223>
OTHER INFORMATION: hVK3LF2 <400> SEQUENCE: 16 accagcgcag
cttctcttcc tcctgctact ctggctccca gataccaccg 50 <210> SEQ ID
NO 17 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Homo sapiens <220> FEATURE: <223> OTHER
INFORMATION: hVK3LF12HVL <400> SEQUENCE: 17 ggctcccaga
taccaccggt caaattgttc tctcccagtc tccag 45 <210> SEQ ID NO 18
<211> LENGTH: 35 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - 2H7VHNheF <400> SEQUENCE: 18
gcgatagcta gccaggctta tctacagcag tctgg 35 <210> SEQ ID NO 19
<211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G4SNheR <400> SEQUENCE: 19
gcgatagcta gccccacctc ctccagatcc accaccgccc gag 43 <210> SEQ
ID NO 20 <211> LENGTH: 35 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 015VHXhoR <400>
SEQUENCE: 20 gcgtactcga ggagacggtg accgtggtcc ctgtg 35 <210>
SEQ ID NO 21 <211> LENGTH: 37 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G1HC-Xho
<400> SEQUENCE: 21 gcagtctcga gcgagcccaa atcttgtgac aaaactc
37 <210> SEQ ID NO 22 <211> LENGTH: 37 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
G1H-S-Xho <400> SEQUENCE: 22 gcagtctcga gcgagcccaa atcttctgac
aaaactc 37 <210> SEQ ID NO 23 <211> LENGTH: 36
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - CH3REcoR1 <400> SEQUENCE: 23 gcgtgagaat tcttacccgg
agacagggag aggctc 36 <210> SEQ ID NO 24 <211> LENGTH:
32 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G1 XBZ-R <400> SEQUENCE: 24 gcgacgtcta gagtcattta
cccggagaca gg 32 <210> SEQ ID NO 25 <211> LENGTH: 54
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G4SLinkR1-S <400> SEQUENCE: 25 aattatggtg gcggtggctc
gggcggtggt ggatctggag gaggtgggag tggg 54 <210> SEQ ID NO 26
<211> LENGTH: 54 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G4SLinkR1-AS <400> SEQUENCE:
26 aattcccact cccacctcct ccagatccac caccgcccga gccaccgcca ccat 54
<210> SEQ ID NO 27 <211> LENGTH: 36 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VLXbaR
<400> SEQUENCE: 27 gcgtgtctag attaacgttt gatttccagc ttggtg 36
<210> SEQ ID NO 28 <211> LENGTH: 35 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VLR1F
<400> SEQUENCE: 28 gcgatgaatt ctgacattgt gctcacccaa tctcc 35
<210> SEQ ID NO 29 <211> LENGTH: 34 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VHR1F
<400> SEQUENCE: 29 gcgatgaatt ctcaggtgca gctgaaggag tcag 34
<210> SEQ ID NO 30 <211> LENGTH: 37 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VHXbaR
<400> SEQUENCE: 30 gcgagtctag attaagagga gacggtgact gaggttc
37 <210> SEQ ID NO 31 <211> LENGTH: 27 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
2e12VHdXbaF1 <400> SEQUENCE: 31 gggtctggag tggctgggaa tgatatg
27 <210> SEQ ID NO 32 <211> LENGTH: 28 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
2e12VHdXbaR1 <400> SEQUENCE: 32 attcccagcc actccagacc
ctttcctg 28 <210> SEQ ID NO 33 <211> LENGTH: 23
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - IgBsrG1F <400> SEQUENCE: 33 gagaaccaca ggtgtacacc
ctg 23 <210> SEQ ID NO 34 <211> LENGTH: 25 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - IgBsrG1R
<400> SEQUENCE: 34 gcagggtgta cacctgtggt tctcg 25 <210>
SEQ ID NO 35 <211> LENGTH: 17 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - M13R <400>
SEQUENCE: 35 caggaaacag ctatgac 17 <210> SEQ ID NO 36
<211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - M13F <400> SEQUENCE: 36
gtaaaacgac ggccagtg 18 <210> SEQ ID NO 37 <211> LENGTH:
20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - T7 <400> SEQUENCE: 37 gtaatacgac tcactatagg 20
<210> SEQ ID NO 38 <211> LENGTH: 17 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - pD18F-17
<400> SEQUENCE: 38 aactagagaa cccactg 17 <210> SEQ ID
NO 39 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - pD18F-20 <400>
SEQUENCE: 39 gctaactaga gaacccactg 20 <210> SEQ ID NO 40
<211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - pD18F-1 <400> SEQUENCE: 40
atacgactca ctataggg 18 <210> SEQ ID NO 41 <211> LENGTH:
19 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - pD18R-s <400> SEQUENCE: 41 gctctagcat ttaggtgac 19
<210> SEQ ID NO 42 <211> LENGTH: 18 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - CH3seqF1
<400> SEQUENCE: 42 catgaggctc tgcacaac 18 <210> SEQ ID
NO 43 <211> LENGTH: 18 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - CH3seqF2 <400>
SEQUENCE: 43 cctctacagc aagctcac 18 <210> SEQ ID NO 44
<211> LENGTH: 19 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - CH3seqR1 <400> SEQUENCE: 44
ggttcttggt cagctcatc 19 <210> SEQ ID NO 45 <211>
LENGTH: 18 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - CH3seqR2 <400> SEQUENCE: 45 gtgagcttgc
tgtagagg 18 <210> SEQ ID NO 46 <211> LENGTH: 51
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - L1-11R <400> SEQUENCE: 46 gcgatagaat tcccagatcc
accaccgccc gagccaccgc caccataatt c 51 <210> SEQ ID NO 47
<211> LENGTH: 36 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - L1-6R <400> SEQUENCE: 47
gcgatagaat tcccagagcc accgccacca taattc 36 <210> SEQ ID NO 48
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - L3R <400> SEQUENCE: 48
gcgtatgaat tccccgagcc accgccaccc ttacccggag acagg 45 <210>
SEQ ID NO 49 <211> LENGTH: 49 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - L4R <400>
SEQUENCE: 49 gcgtatgaat tcccagatcc accaccgccc gagccaccgc cacccttac
49 <210> SEQ ID NO 50 <211> LENGTH: 44 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - L5R
<400> SEQUENCE: 50 gcgtatgaat tcccgctgcc tcctccccca
gatccaccac cgcc 44 <210> SEQ ID NO 51 <211> LENGTH: 23
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - IgBsrG1F <400> SEQUENCE: 51 gagaaccaca ggtgtacacc
ctg 23 <210> SEQ ID NO 52 <211> LENGTH: 50 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - L-CPPCPR
<400> SEQUENCE: 52 gcgatagaat tcggacaagg tggacacccc
ttacccggag acagggagag 50 <210> SEQ ID NO 53 <211>
LENGTH: 41 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - G281LH-NheR <400> SEQUENCE: 53 actgctgcag
ctggaccgcg ctagctccgc cgccacccga c 41 <210> SEQ ID NO 54
<211> LENGTH: 40 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281LH-NheF <400> SEQUENCE:
54 ggcggagcta gcgcggtcca gctgcagcag tctggacctg 40 <210> SEQ
ID NO 55 <211> LENGTH: 37 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - G281-LH-LPinF <400>
SEQUENCE: 55 gcgatcaccg gtgacatcca gatgactcag tctccag 37
<210> SEQ ID NO 56 <211> LENGTH: 37 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-LH-HXhoR
<400> SEQUENCE: 56 gcgatactcg aggagacggt gactgaggtt ccttgac
37 <210> SEQ ID NO 57 <211> LENGTH: 38 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
G281-LH-LEcoF <400> SEQUENCE: 57 gcgatcgaat tcagacatcc
agatgactca gtctccag 38 <210> SEQ ID NO 58 <211> LENGTH:
43 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G281-LH-HXbaR <400> SEQUENCE: 58 gcgattctag
attaggaaga gacggtgact gaggttcctt gac 43 <210> SEQ ID NO 59
<211> LENGTH: 37 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281-HL-HF <400> SEQUENCE: 59
gcgataaccg gtgcggtcca gctgcagcag tctggac 37 <210> SEQ ID NO
60 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - G281-HL-HR3 <400>
SEQUENCE: 60 gacccaccac cgcccgagcc accgccacca gaagagacgg tgactgaggt
tc 52 <210> SEQ ID NO 61 <211> LENGTH: 49 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
G281-HL-HR2 <400> SEQUENCE: 61 actcccgcct cctcctgatc
cgccgccacc cgacccacca ccgcccgag 49 <210> SEQ ID NO 62
<211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281-HL-HNheR <400> SEQUENCE:
62 gagtcatctg gatgtcgcta gcactcccgc ctcctcctga tc 42 <210>
SEQ ID NO 63 <211> LENGTH: 45 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-HL-LNheF
<400> SEQUENCE: 63 atcaggagga ggcgggagtg ctagcgacat
ccagatgact cagtc 45 <210> SEQ ID NO 64 <211> LENGTH: 37
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G281-HL-LXhoR <400> SEQUENCE: 64 gcgatactcg
agcctttgat ctccagttcg gtgcctc 37 <210> SEQ ID NO 65
<211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281-HL-LXhoR <400> SEQUENCE:
65 gcgatatcta gactcaacct ttgatctcca gttcggtgcc tc 42 <210>
SEQ ID NO 66 <211> LENGTH: 37 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-HL-EcoF
<400> SEQUENCE: 66 gcgatagaat tcgcggtcca gctgcagcag tctggac
37 <210> SEQ ID NO 67 <211> LENGTH: 40 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
194-LH-LF1 <400> SEQUENCE: 67 gcgtatgaac cggtgacatc
cagatgacac agactacatc 40 <210> SEQ ID NO 68 <211>
LENGTH: 50 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LF2 <400> SEQUENCE: 68 atccagatga
cacagactac atcctccctg tctgcctctc tgggagacag 50 <210> SEQ ID
NO 69 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LF3 <400> SEQUENCE:
69 gtctgcctct ctgggagaca gagtcaccat cagttgcagg gcaagtcagg ac 52
<210> SEQ ID NO 70 <211> LENGTH: 50 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LF4
<400> SEQUENCE: 70 gttgcagggc aagtcaggac attcgcaatt
atttaaactg gtatcagcag 50 <210> SEQ ID NO 71 <211>
LENGTH: 50 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LF5 <400> SEQUENCE: 71 atttaaactg
gtatcagcag aaaccagatg gaactgttaa actcctgatc 50 <210> SEQ ID
NO 72 <211> LENGTH: 50 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LF6 <400> SEQUENCE:
72 gaactgttaa actcctgatc tactacacat caagattaca ctcaggagtc 50
<210> SEQ ID NO 73 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LF7
<400> SEQUENCE: 73 caagattaca ctcaggagtc ccatcaaggt
tcagtggcag tgggtctgga ac 52 <210> SEQ ID NO 74 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LR7 <400> SEQUENCE: 74 caggttggca
atggtgagag aataatctgt tccagaccca ctgccactga ac 52 <210> SEQ
ID NO 75 <211> LENGTH: 50 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LR6 <400> SEQUENCE:
75 gcaaaagtaa gtggcaatat cttctggttg caggttggca atggtgagag 50
<210> SEQ ID NO 76 <211> LENGTH: 51 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LR5
<400> SEQUENCE: 76 gaacgtccac ggaagcgtat taccctgttg
gcaaaagtaa gtggcaatat c 51 <210> SEQ ID NO 77 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LR4 <400> SEQUENCE: 77 cgtttggtta
ccagtttggt gcctccaccg aacgtccacg gaagcgtatt ac 52 <210> SEQ
ID NO 78 <211> LENGTH: 46 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LR3 <400> SEQUENCE:
78 accaccgccc gagccaccgc caccccgttt ggttaccagt ttggtg 46
<210> SEQ ID NO 79 <211> LENGTH: 50 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LR2
<400> SEQUENCE: 79 gctagcgctc ccacctcctc cagatccacc
accgcccgag ccaccgccac 50 <210> SEQ ID NO 80 <211>
LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LH-LR1 <400> SEQUENCE: 80 gttgcagctg
gacctcgcta gcgctcccac ctcctccaga tc 42 <210> SEQ ID NO 81
<211> LENGTH: 54 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - 194-LH-HF1 <400> SEQUENCE: 81
gatctggagg aggtgggagc gctagcgagg tccagctgca acagtctgga cctg 54
<210> SEQ ID NO 82 <211> LENGTH: 50 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HF2
<400> SEQUENCE: 82 agctgcaaca gtctggacct gaactggtga
agcctggagc ttcaatgaag 50 <210> SEQ ID NO 83 <211>
LENGTH: 50 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HF3 <400> SEQUENCE: 83 agcctggagc
ttcaatgaag atttcctgca aggcctctgg ttactcattc 50 <210> SEQ ID
NO 84 <211> LENGTH: 53 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HF4 <400> SEQUENCE:
84 gcaaggcctc tggttactca ttcactggct acatcgtgaa ctggctgaag cag 53
<210> SEQ ID NO 85 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HF5
<400> SEQUENCE: 85 atcgtgaact ggctgaagca gagccatgga
aagaaccttg agtggattgg ac 52 <210> SEQ ID NO 86 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HF6 <400> SEQUENCE: 86 gaaccttgag
tggattggac ttattaatcc atacaaaggt cttactacct ac 52 <210> SEQ
ID NO 87 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HR6 <400> SEQUENCE:
87 aatgtggcct tgcccttgaa tttctggttg taggtagtaa gacctttgta tg 52
<210> SEQ ID NO 88 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HR5
<400> SEQUENCE: 88 catgtaggct gtgctggatg acttgtctac
agttaatgtg gccttgccct tg 52 <210> SEQ ID NO 89 <211>
LENGTH: 55 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HR4 <400> SEQUENCE: 89 actgcagagt
cttcagatgt cagactgagg agctccatgt aggctgtgct ggatg 55 <210>
SEQ ID NO 90 <211> LENGTH: 52 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HR3
<400> SEQUENCE: 90 accatagtac ccagatcttg cacagtaata
gactgcagag tcttcagatg tc 52 <210> SEQ ID NO 91 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HR2 <400> SEQUENCE: 91 gcgccccaga
catcgaagta ccagtccgag tcaccatagt acccagatct tg 52 <210> SEQ
ID NO 92 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LH-HR1 <400>
SEQUENCE: 92 gcgaatactc gaggagacgg tgaccgtggt ccctgcgccc cagacatcga
ag 52 <210> SEQ ID NO 93 <211> LENGTH: 42 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
194-HL-HF1 <400> SEQUENCE: 93 gcgtatgaac cggtgaggtc
cagctgcaac agtctggacc tg 42 <210> SEQ ID NO 94 <211>
LENGTH: 55 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HL-HR1 <400> SEQUENCE: 94 accgccacca
gaggagacgg tgaccgtggt ccctgcgccc cagacatcga agtac 55 <210>
SEQ ID NO 95 <211> LENGTH: 52 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HL-HR0
<400> SEQUENCE: 95 acctcctcca gatccaccac cgcccgagcc
accgccacca gaggagacgg tg 52 <210> SEQ ID NO 96 <211>
LENGTH: 49 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HL-LF1 <400> SEQUENCE: 96 gcgggggagg
tggcagtgct agcgacatcc agatgacaca gactacatc 49 <210> SEQ ID NO
97 <211> LENGTH: 34 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HL-LR3Xho <400>
SEQUENCE: 97 gcgaatactc gagcgtttgg ttaccagttt ggtg 34 <210>
SEQ ID NO 98 <211> LENGTH: 37 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HL-LR3Xba
<400> SEQUENCE: 98 gcgatatcta gattaccgtt tggttaccag tttggtg
37 <210> SEQ ID NO 99 <211> LENGTH: 43 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
194-HL-HF1R1 <400> SEQUENCE: 99 gcgtatgaga attcagaggt
ccagctgcaa cagtctggac ctg 43 <210> SEQ ID NO 100 <211>
LENGTH: 41 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LH-LF1R1 <400> SEQUENCE: 100
gcgtatgaga attctgacat ccagatgaca cagactacat c 41 <210> SEQ ID
NO 101 <211> LENGTH: 54 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LH-HR1Xba <400>
SEQUENCE: 101 gcgtatctag attaggagac ggtgaccgtg gtccctgcgc
cccagacatc gaag 54 <210> SEQ ID NO 102 <211> LENGTH:
725 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - G28-1 VLVH (DNA) <400> SEQUENCE: 102
accggtgaca tccagatgac tcagtctcca gcctccctat ctgcatctgt gggagagact
60 gtcaccatca catgtcgaac aagtgaaaat gtttacagtt atttggcttg
gtatcagcag 120 aaacagggaa aatctcctca gctcctggtc tcttttgcaa
aaaccttagc agaaggtgtg 180 ccatcaaggt tcagtggcag tggatcaggc
acacagtttt ctctgaagat cagcagcctg 240 cagcctgaag attctggaag
ttatttctgt caacatcatt ccgataatcc gtggacgttc 300 ggtggaggca
ccgaactgga gatcaaaggt ggcggtggct cgggcggtgg tgggtcgggt 360
ggcggcggat ctgctagcgc agtccagctg cagcagtctg gacctgagct ggaaaagcct
420 ggcgcttcag tgaagatttc ctgcaaggct tctggttact cattcactgg
ctacaatatg 480 aactgggtga agcagaataa tggaaagagc cttgagtgga
ttggaaatat tgatccttat 540 tatggtggta ctacctacaa ccggaagttc
aagggcaagg ccacattgac tgtagacaaa 600 tcctccagca cagcctacat
gcagctcaag agtctgacat ctgaggactc tgcagtctat 660 tactgtgcaa
gatcggtcgg ccctatggac tactggggtc aaggaacctc agtcaccgtc 720 tcgag
725 <210> SEQ ID NO 103 <211> LENGTH: 239 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic polypeptide -
G-28-1 VLVH (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(107) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (108)..(124) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (125)..(239) <223> OTHER
INFORMATION: VH <400> SEQUENCE: 103 Asp Ile Gln Met Thr Gln
Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Glu Thr Val Thr
Ile Thr Cys Arg Thr Ser Glu Asn Val Tyr Ser Tyr 20 25 30 Leu Ala
Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 35 40 45
Ser Phe Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly 50
55 60 Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser Ser Leu Gln
Pro 65 70 75 80 Glu Asp Ser Gly Ser Tyr Phe Cys Gln His His Ser Asp
Asn Pro Trp 85 90 95 Thr Phe Gly Gly Gly Thr Glu Leu Glu Ile Lys
Gly Gly Gly Gly Ser 100 105 110 Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ala Ser Ala Val Gln Leu 115 120 125 Gln Gln Ser Gly Pro Glu Leu
Glu Lys Pro Gly Ala Ser Val Lys Ile 130 135 140 Ser Cys Lys Ala Ser
Gly Tyr Ser Phe Thr Gly Tyr Asn Met Asn Trp 145 150 155 160 Val Lys
Gln Asn Asn Gly Lys Ser Leu Glu Trp Ile Gly Asn Ile Asp 165 170 175
Pro Tyr Tyr Gly Gly Thr Thr Tyr Asn Arg Lys Phe Lys Gly Lys Ala 180
185 190 Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu
Lys 195 200 205 Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala
Arg Ser Val 210 215 220 Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser 225 230 235 <210> SEQ ID NO 104 <211>
LENGTH: 767 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic polynucleotide - G28-1 VHVL (DNA) <400> SEQUENCE:
104 accggtgagg tccagctgca acagtctgga cctgaactgg tgaagcctgg
agcttcaatg 60 aagatttcct gcaaggcctc tggttactca ttcactggct
acatcgtgaa ctggctgaag 120 cagagccatg gaaagaacct tgagtggatt
ggacttatta atccatacaa aggtcttact 180 acctacaacc agaaattcaa
gggcaaggcc acattaactg tagacaagtc atccagcaca 240 gcctacatgg
agctcctcag tctgacatct gaagactctg cagtctatta ctgtgcaaga 300
tctgggtact atggtgactc ggactggtac ttcgatgtct ggggcgcagg gaccacggtc
360 accgtctcct ctggtggcgg tggctcgggc ggtggtggat ctggaggagg
tgggagcggg 420 ggaggtggca gtgctagcga catccagatg acacagacta
catcctccct gtctgcctct 480 ctgggagaca gagtcaccat cagttgcagg
gcaagtcagg acattcgcaa ttatttaaac 540 tggtatcagc agaaaccaga
tggaactgtt aaactcctga tctactacac atcaagatta 600 cactcaggag
tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc 660
attgccaacc tgcaaccaga agatattgcc acttactttt gccaacaggg taatacgctt
720 ccgtggacgt tcggtggagg caccaaactg gtaaccaaac gctcgag 767
<210> SEQ ID NO 105 <211> LENGTH: 253 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polypeptide - G28-1 VHVL
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(121) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (122)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(253) <223> OTHER INFORMATION: VL
<400> SEQUENCE: 105 Glu Val Gln Leu Gln Gln Ser Gly Pro Glu
Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Met Lys Ile Ser Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly Tyr 20 25 30 Ile Val Asn Trp Leu Lys
Gln Ser His Gly Lys Asn Leu Glu Trp Ile 35 40 45 Gly Leu Ile Asn
Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe 50 55 60 Lys Gly
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80
Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val
Trp 100 105 110 Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
Gly Ser Gly 115 120 125 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
Gly Gly Ser Ala Ser 130 135 140 Asp Ile Gln Met Thr Gln Thr Thr Ser
Ser Leu Ser Ala Ser Leu Gly 145 150 155 160 Asp Arg Val Thr Ile Ser
Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 165 170 175 Leu Asn Trp Tyr
Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 180 185 190 Tyr Tyr
Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 195 200 205
Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro 210
215 220 Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro
Trp 225 230 235 240 Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg
Ser 245 250 <210> SEQ ID NO 106 <211> LENGTH: 749
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - G19-4 VLVH (DNA) <400> SEQUENCE: 106
accggtgaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga
60 gtcaccatca gttgcagggc aagtcaggac attcgcaatt atttaaactg
gtatcagcag 120 aaaccagatg gaactgttaa actcctgatc tactacacat
caagattaca ctcaggagtc 180 ccatcaaggt tcagtggcag tgggtctgga
acagattatt ctctcaccat tgccaacctg 240 caaccagaag atattgccac
ttacttttgc caacagggta atacgcttcc gtggacgttc 300 ggtggaggca
ccaaactggt aaccaaacgg ggtggcggtg gctcgggcgg tggtggatct 360
ggaggaggtg ggagcgctag cgaggtccag ctgcaacagt ctggacctga actggtgaag
420 cctggagctt caatgaagat ttcctgcaag gcctctggtt actcattcac
tggctacatc 480 gtgaactggc tgaagcagag ccatggaaag aaccttgagt
ggattggact tattaatcca 540 tacaaaggtc ttactaccta caaccagaaa
ttcaagggca aggccacatt aactgtagac 600 aagtcatcca gcacagccta
catggagctc ctcagtctga catctgaaga ctctgcagtc 660 tattactgtg
caagatctgg gtactatggt gactcggact ggtacttcga tgtctggggc 720
gcagggacca cggtcaccgt ctcctcgag 749 <210> SEQ ID NO 107
<211> LENGTH: 247 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic polypeptide - G-19-4 VLVH (AA) <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(108) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(109)..(125) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(126)..(247) <223> OTHER INFORMATION: VH <400>
SEQUENCE: 107 Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala
Ser Leu Gly 1 5 10 15 Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln
Asp Ile Arg Asn Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Asp
Gly Thr Val Lys Leu Leu Ile 35 40 45 Tyr Tyr Thr Ser Arg Leu His
Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr
Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro 65 70 75 80 Glu Asp Ile
Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp 85 90 95 Thr
Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg Gly Gly Gly Gly 100 105
110 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Glu Val Gln
115 120 125 Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser
Met Lys 130 135 140 Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly
Tyr Ile Val Asn 145 150 155 160 Trp Leu Lys Gln Ser His Gly Lys Asn
Leu Glu Trp Ile Gly Leu Ile 165 170 175 Asn Pro Tyr Lys Gly Leu Thr
Thr Tyr Asn Gln Lys Phe Lys Gly Lys 180 185 190 Ala Thr Leu Thr Val
Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu 195 200 205 Leu Ser Leu
Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser 210 215 220 Gly
Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala Gly 225 230
235 240 Thr Thr Val Thr Val Ser Ser 245 <210> SEQ ID NO 108
<211> LENGTH: 752 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic polynucleotide - G19-4 VHVL (DNA)
<400> SEQUENCE: 108 accggtgagg tccagctgca acagtctgga
cctgaactgg tgaagcctgg agcttcaatg 60 aagatttcct gcaaggcctc
tggttactca ttcactggct acatcgtgaa ctggctgaag 120 cagagccatg
gaaagaacct tgagtggatt ggacttatta atccatacaa aggtcttact 180
acctacaacc agaaattcaa gggcaaggcc acattaactg tagacaagtc atccagcaca
240 gcctacatgg agctcctcag tctgacatct gaagactctg cagtctatta
ctgtgcaaga 300 tctgggtact atggtgactc ggactggtac ttcgatgtct
ggggcgcagg gaccacggtc 360 accgtctcct ctggtggcgg tggctcgggc
ggtggtggat ctggaggagg tgggagcgct 420 agcgacatcc agatgacaca
gactacatcc tccctgtctg cctctctggg agacagagtc 480 accatcagtt
gcagggcaag tcaggacatt cgcaattatt taaactggta tcagcagaaa 540
ccagatggaa ctgttaaact cctgatctac tacacatcaa gattacactc aggagtccca
600 tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattgc
caacctgcaa 660 ccagaagata ttgccactta cttttgccaa cagggtaata
cgcttccgtg gacgttcggt 720 ggaggcacca aactggtaac caaacgctcg ag 752
<210> SEQ ID NO 109 <211> LENGTH: 248 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polypeptide - G19-4 VHVL
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(122) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (123)..(139) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (140)..(248) <223> OTHER INFORMATION: VL
<400> SEQUENCE: 109 Glu Val Gln Leu Gln Gln Ser Gly Pro Glu
Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Met Lys Ile Ser Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly Tyr 20 25 30 Ile Val Asn Trp Leu Lys
Gln Ser His Gly Lys Asn Leu Glu Trp Ile 35 40 45 Gly Leu Ile Asn
Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe 50 55 60 Lys Gly
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80
Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val
Trp 100 105 110 Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
Gly Ser Gly 115 120 125 Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser
Asp Ile Gln Met Thr 130 135 140 Gln Thr Thr Ser Ser Leu Ser Ala Ser
Leu Gly Asp Arg Val Thr Ile 145 150 155 160 Ser Cys Arg Ala Ser Gln
Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln 165 170 175 Gln Lys Pro Asp
Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Arg 180 185 190 Leu His
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 195 200 205
Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu Asp Ile Ala Thr 210
215 220 Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly
Gly 225 230 235 240 Thr Lys Leu Val Thr Lys Arg Ser 245 <210>
SEQ ID NO 110 <211> LENGTH: 45 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(s)-hIgG1 (DNA) <400> SEQUENCE: 110
gagcccaaat cttctgacaa aactcacaca tctccaccgt gctca 45 <210>
SEQ ID NO 111 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide - ssc(s)-hIgG1
(AA) <400> SEQUENCE: 111 Glu Pro Lys Ser Ser Asp Lys Thr His
Thr Ser Pro Pro Cys Ser 1 5 10 15 <210> SEQ ID NO 112
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: scc(s)-hIgG1
(DNA) <400> SEQUENCE: 112 gagcccaaat cttctgacaa aactcacaca
tgtccaccgt gctca 45 <210> SEQ ID NO 113 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
peptide - scc(s)-hIgG1 (AA) <400> SEQUENCE: 113 Glu Pro Lys
Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Ser 1 5 10 15
<210> SEQ ID NO 114 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: css(s)-hIgG1 (DNA) <400> SEQUENCE: 114
gagcccaaat cttgtgacaa aactcacaca tctccaccga gctca 45 <210>
SEQ ID NO 115 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide - css(s)-hIgG1
(AA) <400> SEQUENCE: 115 Glu Pro Lys Ser Cys Asp Lys Thr His
Thr Ser Pro Pro Ser Ser 1 5 10 15 <210> SEQ ID NO 116
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic polynucleotide <220> FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
scs(s)-hIgG1 (DNA) <400> SEQUENCE: 116 gagcccaaat cttgtgacaa
aactcacaca tgtccaccga gctca 45 <210> SEQ ID NO 117
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic peptide - scs(s)-hIgG1 (AA) <400>
SEQUENCE: 117 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro
Ser Ser 1 5 10 15 <210> SEQ ID NO 118 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer <400> SEQUENCE: 118 gagcccaaat cttgtgacaa aactcacaca
tgtccaccgt gctca 45 <210> SEQ ID NO 119 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <400> SEQUENCE: 119 Glu Pro Lys Ser Cys Asp Lys Thr
His Thr Cys Pro Pro Cys Ser 1 5 10 15 <210> SEQ ID NO 120
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <400> SEQUENCE: 120 gagcccaaat
cttgtgacaa aactcacaca tgtccaccgt gccca 45 <210> SEQ ID NO 121
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <400> SEQUENCE: 121 Glu
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 122 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <400>
SEQUENCE: 122 gagcccaaat cttctgacaa aactcacaca tctccaccga gccca 45
<210> SEQ ID NO 123 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <400>
SEQUENCE: 123 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro
Ser Pro 1 5 10 15 <210> SEQ ID NO 124 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer <220> FEATURE: <221> NAME/KEY: misc_feature
<223> OTHER INFORMATION: csc(p)-hIgG1 (DNA) <400>
SEQUENCE: 124 gagcccaaat cttgtgacaa aactcacaca tctccaccgt gccca 45
<210> SEQ ID NO 125 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: csc(p)-hIgG1 (AA) <400> SEQUENCE: 125 Glu Pro
Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 126 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (DNA) <400> SEQUENCE: 126
gagcccaaat cttctgacaa aactcacaca tctccaccgt gccca 45 <210>
SEQ ID NO 127 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (AA) <400> SEQUENCE: 127 Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 128 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: scc(p)-hIgG1 (DNA) <400> SEQUENCE: 128
gagcccaaat cttctgacaa aactcacaca tgtccaccgt gccca 45 <210>
SEQ ID NO 129 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: scc(p)-hIgG1 (AA) <400> SEQUENCE: 129 Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 130 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: css(p)-hIgG1 (DNA) <400> SEQUENCE: 130
gagcccaaat cttgtgacaa aactcacaca tctccaccga gccca 45 <210>
SEQ ID NO 131 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: css(p)-hIgG1 (AA) <400> SEQUENCE: 131 Glu Pro
Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Ser Pro 1 5 10 15
<210> SEQ ID NO 132 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (DNA) <400> SEQUENCE: 132
gagcccaaat cttgtgacaa aactcacaca tgtccaccga gccca 45 <210>
SEQ ID NO 133 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (DNA) <400> SEQUENCE: 133 Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Ser Pro 1 5 10 15
<210> SEQ ID NO 134 <211> LENGTH: 18 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
ssc(p) (DNA) <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <400> SEQUENCE: 134 agttgtccac cgtgccca 18
<210> SEQ ID NO 135 <211> LENGTH: 6 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <400>
SEQUENCE: 135 Ser Cys Pro Pro Cys Pro 1 5 <210> SEQ ID NO 136
<211> LENGTH: 7 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <400> SEQUENCE: 136 tgatcag 7
<210> SEQ ID NO 137 <211> LENGTH: 2 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide <400>
SEQUENCE: 137 Asp Gln 1 <210> SEQ ID NO 138 <211>
LENGTH: 7 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <400> SEQUENCE: 138 ctcgagt 7 <210>
SEQ ID NO 139 <211> LENGTH: 2 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide <400>
SEQUENCE: 139 Ser Ser 1 <210> SEQ ID NO 140 <211>
LENGTH: 654 <212> TYPE: DNA <213> ORGANISM: Homo
sapiens <220> FEATURE: <223> OTHER INFORMATION: hIgG1
wild type <400> SEQUENCE: 140 gcacctgaac tcctgggtgg
accgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60 ctcatgatct
cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120
cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag
180 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac
cgtcctgcac 240 caggactggc tgaatggcaa ggagtacaag tgcaaggtct
ccaacaaagc cctcccagcc 300 cccatcgaga aaacaatctc caaagccaaa
gggcagcccc gagaaccaca ggtgtacacc 360 ctgcccccat cccgggatga
gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420 ggcttctatc
ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac 480
tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc
540 accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt
gatgcatgag 600 gctctgcaca accactacac gcagaagagc ctctccctgt
ctccgggtaa atga 654 <210> SEQ ID NO 141 <211> LENGTH:
217 <212> TYPE: PRT <213> ORGANISM: Homo sapiens
<220> FEATURE: <223> OTHER INFORMATION: hIgG1 wild type
<400> SEQUENCE: 141 Ala Pro Glu Leu Leu Gly Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp Thr Leu Met Ile Ser
Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val Val Asp Val Ser His
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 Val Asp Gly Val
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55 60 Gln Tyr
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 65 70 75 80
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 85
90 95 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
Gln 100 105 110 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu 115 120 125 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro 130 135 140 Ser Asp Ile Ala Val Glu Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 Tyr Lys Thr Thr Pro Pro
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175 Tyr Ser Lys Leu
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185 190 Phe Ser
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 195 200 205
Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215 <210> SEQ ID NO
142 <211> LENGTH: 654 <212> TYPE: DNA <213>
ORGANISM: Homo sapiens <220> FEATURE: <223> OTHER
INFORMATION: hIgG1 (P238S) <400> SEQUENCE: 142 gcacctgaac
tcctgggtgg atcgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60
ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac
120 cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc
caagacaaag 180 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca
gcgtcctcac cgtcctgcac 240 caggactggc tgaatggcaa ggagtacaag
tgcaaggtct ccaacaaagc cctcccagcc 300 cccatcgaga aaacaatctc
caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 360 ctgcccccat
cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420
ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac
480 tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta
cagcaagctc 540 accgtggaca agagcaggtg gcagcagggg aacgtcttct
catgctccgt gatgcatgag 600 gctctgcaca accactacac gcagaagagc
ctctccctgt ctccgggtaa atga 654 <210> SEQ ID NO 143
<211> LENGTH: 217 <212> TYPE: PRT <213> ORGANISM:
Homo sapiens <220> FEATURE: <223> OTHER INFORMATION:
hIgG1 (P238S) <400> SEQUENCE: 143 Ala Pro Glu Leu Leu Gly Gly
Ser Ser Val Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp Thr Leu
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val Val Asp
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 Val
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55
60 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
65 70 75 80 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys 85 90 95 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln 100 105 110 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
Pro Ser Arg Asp Glu Leu 115 120 125 Thr Lys Asn Gln Val Ser Leu Thr
Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140 Ser Asp Ile Ala Val Glu
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 Tyr Lys Thr
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175 Tyr
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185
190 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
195 200 205 Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215 <210>
SEQ ID NO 144 <211> LENGTH: 654 <212> TYPE: DNA
<213> ORGANISM: Homo sapiens <220> FEATURE: <223>
OTHER INFORMATION: hIgG1 (P331S) <400> SEQUENCE: 144
gcacctgaac tcctgggtgg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc
60 ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag
ccacgaagac 120 cctgaggtca agttcaactg gtacgtggac ggcgtggagg
tgcataatgc caagacaaag 180 ccgcgggagg agcagtacaa cagcacgtac
cgtgtggtca gcgtcctcac cgtcctgcac 240 caggactggc tgaatggcaa
ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc 300 tccatcgaga
aaacaatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 360
ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa
420 ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc
ggagaacaac 480 tacaagacca cgcctcccgt gctggactcc gacggctcct
tcttcctcta cagcaagctc 540 accgtggaca agagcaggtg gcagcagggg
aacgtcttct catgctccgt gatgcatgag 600 gctctgcaca accactacac
gcagaagagc ctctccctgt ctccgggtaa atga 654 <210> SEQ ID NO 145
<211> LENGTH: 217 <212> TYPE: PRT <213> ORGANISM:
Homo sapiens <220> FEATURE: <223> OTHER INFORMATION:
hIgG1 (P331S) <400> SEQUENCE: 145 Ala Pro Glu Leu Leu Gly Gly
Pro Ser Val Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp Thr Leu
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val Val Asp
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 Val
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55
60 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
65 70 75 80 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys 85 90 95 Ala Leu Pro Ala Ser Ile Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln 100 105 110 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
Pro Ser Arg Asp Glu Leu 115 120 125 Thr Lys Asn Gln Val Ser Leu Thr
Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140 Ser Asp Ile Ala Val Glu
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 Tyr Lys Thr
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175 Tyr
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185
190 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
195 200 205 Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215 <210>
SEQ ID NO 146 <211> LENGTH: 654 <212> TYPE: DNA
<213> ORGANISM: Homo sapiens <220> FEATURE: <223>
OTHER INFORMATION: hIgG1 (P238S/P331S) <400> SEQUENCE: 146
gcacctgaac tcctgggtgg atcgtcagtc ttcctcttcc ccccaaaacc caaggacacc
60 ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag
ccacgaagac 120 cctgaggtca agttcaactg gtacgtggac ggcgtggagg
tgcataatgc caagacaaag 180 ccgcgggagg agcagtacaa cagcacgtac
cgtgtggtca gcgtcctcac cgtcctgcac 240 caggactggc tgaatggcaa
ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc 300 tccatcgaga
aaacaatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 360
ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa
420 ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc
ggagaacaac 480 tacaagacca cgcctcccgt gctggactcc gacggctcct
tcttcctcta cagcaagctc 540 accgtggaca agagcaggtg gcagcagggg
aacgtcttct catgctccgt gatgcatgag 600 gctctgcaca accactacac
gcagaagagc ctctccctgt ctccgggtaa atga 654 <210> SEQ ID NO 147
<211> LENGTH: 217 <212> TYPE: PRT <213> ORGANISM:
Homo sapiens <220> FEATURE: <223> OTHER INFORMATION:
hIgG1 (P238S/P331S) <400> SEQUENCE: 147 Ala Pro Glu Leu Leu
Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40
45 Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
50 55 60 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
Leu His 65 70 75 80 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
Val Ser Asn Lys 85 90 95 Ala Leu Pro Ala Ser Ile Glu Lys Thr Ile
Ser Lys Ala Lys Gly Gln 100 105 110 Pro Arg Glu Pro Gln Val Tyr Thr
Leu Pro Pro Ser Arg Asp Glu Leu 115 120 125 Thr Lys Asn Gln Val Ser
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140 Ser Asp Ile Ala
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 Tyr
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170
175 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
180 185 190 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
Thr Gln 195 200 205 Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215
<210> SEQ ID NO 148 <211> LENGTH: 60 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <223> OTHER INFORMATION: STD1 (DNA) <400>
SEQUENCE: 148 aattatggtg gcggtggctc gggcggtggt ggatctggag
gaggtgggag tgggaattct 60 <210> SEQ ID NO 149 <211>
LENGTH: 20 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion linker region <220> FEATURE: <223> OTHER
INFORMATION: STD1 (AA) <400> SEQUENCE: 149 Asn Tyr Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 1 5 10 15 Ser Gly
Asn Ser 20 <210> SEQ ID NO 150 <211> LENGTH: 114
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer <220> FEATURE: <223> OTHER INFORMATION: STD2
(DNA) <400> SEQUENCE: 150 aattatggtg gcggtggctc gggcggtggt
ggatctggag gaggtgggag tgggaattat 60 ggtggcggtg gctcgggcgg
tggtggatct ggaggaggtg ggagtgggaa ttct 114 <210> SEQ ID NO 151
<211> LENGTH: 38 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <220> FEATURE:
<223> OTHER INFORMATION: STD2 (AA) <400> SEQUENCE: 151
Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 1 5
10 15 Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly 20 25 30 Gly Gly Ser Gly Asn Ser 35 <210> SEQ ID NO 152
<211> LENGTH: 6 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <220> FEATURE: <223>
OTHER INFORMATION: Linker H1 (PN) <400> SEQUENCE: 152 aattct
6 <210> SEQ ID NO 153 <211> LENGTH: 2 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker H1 (AA) <400>
SEQUENCE: 153 Asn Ser 1 <210> SEQ ID NO 154 <211>
LENGTH: 24 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <220> FEATURE: <223> OTHER
INFORMATION: Linker H2 (PN) <400> SEQUENCE: 154 ggtggcggtg
gctcggggaa ttct 24 <210> SEQ ID NO 155 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <220> FEATURE: <223> OTHER INFORMATION: Linker
H2 (AA) <400> SEQUENCE: 155 Gly Gly Gly Gly Ser Gly Asn Ser 1
5 <210> SEQ ID NO 156 <211> LENGTH: 30 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer
<220> FEATURE: <223> OTHER INFORMATION: Linker H3 (PN)
<400> SEQUENCE: 156 aattatggtg gcggtggctc tgggaattct 30
<210> SEQ ID NO 157 <211> LENGTH: 10 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker H3 (AA) <400>
SEQUENCE: 157 Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser 1 5 10
<210> SEQ ID NO 158 <211> LENGTH: 39 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <223> OTHER INFORMATION: Linker H4 (PN) <400>
SEQUENCE: 158 ggtggcggtg gctcgggcgg tggtggatct gggaattct 39
<210> SEQ ID NO 159 <211> LENGTH: 13 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker H4 (AA) <400>
SEQUENCE: 159 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser 1
5 10 <210> SEQ ID NO 160 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer
<220> FEATURE: <223> OTHER INFORMATION: Linker H5 (PN)
<400> SEQUENCE: 160 aattatggtg gcggtggctc gggcggtggt
ggatctggga attct 45 <210> SEQ ID NO 161 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <220> FEATURE: <223> OTHER INFORMATION: Linker
H5 (AA) <400> SEQUENCE: 161 Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly Asn Ser 1 5 10 15 <210> SEQ ID NO 162
<211> LENGTH: 54 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <220> FEATURE: <223>
OTHER INFORMATION: Linker H6 (PN) <400> SEQUENCE: 162
ggtggcggtg gctcgggcgg tggtggatct gggggaggag gcagcgggaa ttct 54
<210> SEQ ID NO 163 <211> LENGTH: 18 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker H6 (AA) <400>
SEQUENCE: 163 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly 1 5 10 15 Asn Ser <210> SEQ ID NO 164 <211>
LENGTH: 24 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <220> FEATURE: <223> OTHER
INFORMATION: Linker H7 (PN) <400> SEQUENCE: 164 gggtgtccac
cttgtccgaa ttct 24 <210> SEQ ID NO 165 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <220> FEATURE: <223> OTHER INFORMATION: Linker
H7 (AA) <400> SEQUENCE: 165 Gly Cys Pro Pro Cys Pro Asn Ser 1
5 <210> SEQ ID NO 166 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer
<220> FEATURE: <223> OTHER INFORMATION: Linker(G4S)3
<400> SEQUENCE: 166 ggtggcggtg gatccggcgg aggtgggtcg
ggtggcggcg gatct 45 <210> SEQ ID NO 167 <211> LENGTH:
12 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <220> FEATURE: <223> OTHER INFORMATION:
Linker(G4S)3 <400> SEQUENCE: 167 Gly Gly Gly Ser Gly Gly Gly
Ser Gly Gly Gly Ser 1 5 10 <210> SEQ ID NO 168 <211>
LENGTH: 60 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <220> FEATURE: <223> OTHER
INFORMATION: Linker(G4S)4 <400> SEQUENCE: 168 ggtggcggtg
gctcgggcgg tggtggatct ggaggaggtg ggagcggggg aggtggcagt 60
<210> SEQ ID NO 169 <211> LENGTH: 17 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker(G4S)4 <400>
SEQUENCE: 169 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly
Gly Gly Gly 1 5 10 15 Ser <210> SEQ ID NO 170 <211>
LENGTH: 2337 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-STD1-2e12HL (DNA) <400> SEQUENCE: 170 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagctcagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt
1560 gggaattctc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc
ctcacagagc 1620 ctgtccatca catgcaccgt ctcagggttc tcattaaccg
gctatggtgt aaactgggtt 1680 cgccagcctc caggaaaggg tctggagtgg
ctgggaatga tatggggtga tggaagcaca 1740 gactataatt cagctctcaa
atccagacta tcgatcacca aggacaactc caagagccaa 1800 gttttcttaa
aaatgaacag tctgcaaact gatgacacag ccagatacta ctgtgctcga 1860
gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc
1920 accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg
tgggtcgggt 1980 ggcggcggat ctgacattgt gctcacccaa tctccagctt
ctttggctgt gtctctaggt 2040 cagagagcca ccatctcctg cagagccagt
gaaagtgttg aatattatgt cacaagttta 2100 atgcagtggt accaacagaa
accaggacag ccacccaaac tcctcatctc tgctgctagc 2160 aacgtagaat
ctggggtccc tgccaggttt agtggcagtg ggtctgggac agactttagc 2220
ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca gcaaagtagg
2280 aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgtta atctaga
2337 <210> SEQ ID NO 171 <211> LENGTH: 772 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2H7sssIgG1-STD1-2e12HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(281) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(519) <223> OTHER INFORMATION: EFD-BD2 linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (520)..(640) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (641)..(660) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (661)..(772) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 171 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Gln Val
Gln Leu Lys Glu Ser Gly Pro 515 520 525 Gly Leu Val Ala Pro Ser Gln
Ser Leu Ser Ile Thr Cys Thr Val Ser 530 535 540 Gly Phe Ser Leu Thr
Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 545 550 555 560 Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 565 570 575
Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 580
585 590 Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp
Asp 595 600 605 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn
Phe His Tyr 610 615 620 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Gly 645 650 655 Gly Gly Gly Ser Asp Ile
Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 660 665 670 Val Ser Leu Gly
Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 675 680 685 Val Glu
Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 690 695 700
Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 705
710 715 720 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp
Phe Ser 725 730 735 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala
Met Tyr Phe Cys 740 745 750 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe
Gly Gly Gly Thr Lys Leu 755 760 765 Glu Ile Lys Arg 770 <210>
SEQ ID NO 172 <211> LENGTH: 772 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1(P238S/P331S)-STD1-2e12HL
(w/2e12 leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(282) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(519) <223> OTHER
INFORMATION: EFD-BD2 linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (520)..(640)
<223> OTHER INFORMATION: VH2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (641)..(660)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(661)..(772) <223> OTHER INFORMATION: VL2 <400>
SEQUENCE: 172 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys
Glu Ser Gly Pro 515 520 525 Gly Leu Val Ala Pro Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser 530 535 540 Gly Phe Ser Leu Thr Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro 545 550 555 560 Gly Lys Gly Leu Glu
Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 565 570 575 Asp Tyr Asn
Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 580 585 590 Ser
Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 595 600
605 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
610 615 620 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 645 650 655 Gly Gly Gly Ser Asp Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala 660 665 670 Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala Ser Glu Ser 675 680 685 Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 690 695 700 Gly Gln Pro
Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 705 710 715 720
Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 725
730 735 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe
Cys 740 745 750 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly
Thr Lys Leu 755 760 765 Glu Ile Lys Arg 770 <210> SEQ ID NO
173 <211> LENGTH: 2322 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-STD1-2e12LH (DNA) <400>
SEQUENCE: 173 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgagcccagc
acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg cggtggctcg
ggcggtggtg gatctggagg aggtgggagt 1560 gggaattctg acattgtgct
cacccaatct ccagcttctt tggctgtgtc tctaggtcag 1620 agagccacca
tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg 1680
cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc tgctagcaac
1740 gtagaatctg gggtccctgc caggtttagt ggcagtgggt ctgggacaga
ctttagcctc 1800 aacatccatc ctgtggagga ggatgatatt gcaatgtatt
tctgtcagca aagtaggaag 1860 gttccatgga cgttcggtgg aggcaccaag
ctggaaatca aacggggtgg cggtggatcc 1920 ggcggaggtg ggtcgggtgg
cggcggatct caggtgcagc tgaaggagtc aggacctggc 1980 ctggtggcgc
cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc 2040
ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg gctgggaatg
2100 atatggggtg atggaagcac agactataat tcagctctca aatccagact
atcgatcacc 2160 aaggacaact ccaagagcca agttttctta aaaatgaaca
gtctgcaaac tgatgacaca 2220 gccagatact actgtgctcg agatggttat
agtaactttc attactatgt tatggactac 2280 tggggtcaag gaacctcagt
caccgtctcc tcttaatcta ga 2322 <210> SEQ ID NO 174 <211>
LENGTH: 767 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-STD1-2e12LH (w/2e12 leader) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(519) <223> OTHER INFORMATION: EFD-BD2 linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (520)..(631) <223> OTHER INFORMATION: VL2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (632)..(646) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (647)..(767) <223> OTHER INFORMATION: VH2
<400> SEQUENCE: 174 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Asp Ile
Val Leu Thr Gln Ser Pro Ala 515 520 525 Ser Leu Ala Val Ser Leu Gly
Gln Arg Ala Thr Ile Ser Cys Arg Ala 530 535 540 Ser Glu Ser Val Glu
Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 545 550 555 560 Gln Lys
Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 565 570 575
Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 580
585 590 Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala
Met 595 600 605 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe
Gly Gly Gly 610 615 620 Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly
Ser Gly Gly Gly Gly 625 630 635 640 Ser Gly Gly Gly Gly Ser Gln Val
Gln Leu Lys Glu Ser Gly Pro Gly 645 650 655 Leu Val Ala Pro Ser Gln
Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 660 665 670 Phe Ser Leu Thr
Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly 675 680 685 Lys Gly
Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 690 695 700
Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 705
710 715 720 Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp
Asp Thr 725 730 735 Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn
Phe His Tyr Tyr 740 745 750 Val Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser Ser 755 760 765 <210> SEQ ID NO 175
<211> LENGTH: 767 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: 2H7sssIgG1(P238S/P331S)-STD1-2e12LH (w/2e12 leader)
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(22) <223> OTHER INFORMATION:
Leader <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (23)..(128) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (129)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(519) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (520)..(631) <223> OTHER INFORMATION:
VL2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (632)..(646) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (647)..(767) <223> OTHER INFORMATION:
VH2 <400> SEQUENCE: 175 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Ser Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440
445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys
Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser
Asp Ile Val Leu Thr Gln Ser Pro Ala 515 520 525 Ser Leu Ala Val Ser
Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 530 535 540 Ser Glu Ser
Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 545 550 555 560
Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 565
570 575 Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly
Thr 580 585 590 Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp
Ile Ala Met 595 600 605 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp
Thr Phe Gly Gly Gly 610 615 620 Thr Lys Leu Glu Ile Lys Arg Gly Gly
Gly Gly Ser Gly Gly Gly Gly 625 630 635 640 Ser Gly Gly Gly Gly Ser
Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 645 650 655 Leu Val Ala Pro
Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 660 665 670 Phe Ser
Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly 675 680 685
Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 690
695 700 Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn
Ser 705 710 715 720 Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln
Thr Asp Asp Thr 725 730 735 Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr
Ser Asn Phe His Tyr Tyr 740 745 750 Val Met Asp Tyr Trp Gly Gln Gly
Thr Ser Val Thr Val Ser Ser 755 760 765 <210> SEQ ID NO 176
<211> LENGTH: 2376 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-STD2-2e12LH (DNA) <400>
SEQUENCE: 176 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgagcccagc
acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg cggtggctcg
ggcggtggtg gatctggagg aggtgggagt 1560 gggaattatg gtggcggtgg
ctcgggcggt ggtggatctg gaggaggtgg gagtgggaat 1620 tctgacattg
tgctcaccca atctccagct tctttggctg tgtctctagg tcagagagcc 1680
accatctcct gcagagccag tgaaagtgtt gaatattatg tcacaagttt aatgcagtgg
1740 taccaacaga aaccaggaca gccacccaaa ctcctcatct ctgctgctag
caacgtagaa 1800 tctggggtcc ctgccaggtt tagtggcagt gggtctggga
cagactttag cctcaacatc 1860 catcctgtgg aggaggatga tattgcaatg
tatttctgtc agcaaagtag gaaggttcca 1920 tggacgttcg gtggaggcac
caagctggaa atcaaacggg gtggcggtgg atccggcgga 1980 ggtgggtcgg
gtggcggcgg atctcaggtg cagctgaagg agtcaggacc tggcctggtg 2040
gcgccctcac agagcctgtc catcacatgc accgtctcag ggttctcatt aaccggctat
2100 ggtgtaaact gggttcgcca gcctccagga aagggtctgg agtggctggg
aatgatatgg 2160 ggtgatggaa gcacagacta taattcagct ctcaaatcca
gactatcgat caccaaggac 2220 aactccaaga gccaagtttt cttaaaaatg
aacagtctgc aaactgatga cacagccaga 2280 tactactgtg ctcgagatgg
ttatagtaac tttcattact atgttatgga ctactggggt 2340 caaggaacct
cagtcaccgt ctcctcttaa tctaga 2376 <210> SEQ ID NO 177
<211> LENGTH: 785 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: 2H7sssIgG1-STD2-2e12LH (w/2e12 leader) <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(22) <223> OTHER INFORMATION: Leader <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(129) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(130)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(537) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (538)..(649) <223> OTHER INFORMATION: VL2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (650)..(664) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (665)..(785) <223> OTHER INFORMATION: VH2
<400> SEQUENCE: 177 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr Gly Gly
Gly Gly Ser Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly Ser Gly
Asn Ser Asp Ile Val Leu Thr Gln Ser 530 535 540 Pro Ala Ser Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 545 550 555 560 Arg Ala
Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp 565 570 575
Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala 580
585 590 Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly
Ser 595 600 605 Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp Ile 610 615 620 Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe Gly 625 630 635 640 Gly Gly Thr Lys Leu Glu Ile Lys
Arg Gly Gly Gly Gly Ser Gly Gly 645 650 655 Gly Gly Ser Gly Gly Gly
Gly Ser Gln Val Gln Leu Lys Glu Ser Gly 660 665 670 Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val 675 680 685 Ser Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 690 695 700
Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser 705
710 715 720 Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys Asp 725 730 735 Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr Asp 740 745 750 Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser Asn Phe His 755 760 765 Tyr Tyr Val Met Asp Tyr Trp Gly
Gln Gly Thr Ser Val Thr Val Ser 770 775 780 Ser 785 <210> SEQ
ID NO 178 <211> LENGTH: 785 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1(P238S/P331S)-STD2-2e12LH (w/2e12
leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(281) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(537) <223> OTHER
INFORMATION: EFD-BD2 Linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (538)..(649)
<223> OTHER INFORMATION: VL2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (650)..(664)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(665)..(785) <223> OTHER INFORMATION: VH2 <400>
SEQUENCE: 178 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr Gly Gly Gly Gly Ser
Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly Ser Gly Asn Ser Asp
Ile Val Leu Thr Gln Ser 530 535 540 Pro Ala Ser Leu Ala Val Ser Leu
Gly Gln Arg Ala Thr Ile Ser Cys 545 550 555 560 Arg Ala Ser Glu Ser
Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp 565 570 575 Tyr Gln Gln
Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala 580 585 590 Ser
Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser 595 600
605 Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile
610 615 620 Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr
Phe Gly 625 630 635 640 Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly
Gly Gly Ser Gly Gly 645 650 655 Gly Gly Ser Gly Gly Gly Gly Ser Gln
Val Gln Leu Lys Glu Ser Gly 660 665 670 Pro Gly Leu Val Ala Pro Ser
Gln Ser Leu Ser Ile Thr Cys Thr Val 675 680 685 Ser Gly Phe Ser Leu
Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 690 695 700 Pro Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser 705 710 715 720
Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp 725
730 735 Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr
Asp 740 745 750 Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser
Asn Phe His 755 760 765 Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr
Ser Val Thr Val Ser 770 775 780 Ser 785 <210> SEQ ID NO 179
<211> LENGTH: 2391 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-STD2-2e12HL (DNA) <400>
SEQUENCE: 179 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgagcccagc
acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg cggtggctcg
ggcggtggtg gatctggagg aggtgggagt 1560 gggaattatg gtggcggtgg
ctcgggcggt ggtggatctg gaggaggtgg gagtgggaat 1620 tctcaggtgc
agctgaagga gtcaggacct ggcctggtgg cgccctcaca gagcctgtcc 1680
atcacatgca ccgtctcagg gttctcatta accggctatg gtgtaaactg ggttcgccag
1740 cctccaggaa agggtctgga gtggctggga atgatatggg gtgatggaag
cacagactat 1800 aattcagctc tcaaatccag actatcgatc accaaggaca
actccaagag ccaagttttc 1860 ttaaaaatga acagtctgca aactgatgac
acagccagat actactgtgc tcgagatggt 1920 tatagtaact ttcattacta
tgttatggac tactggggtc aaggaacctc agtcaccgtc 1980 tcctctgggg
gtggaggctc tggtggcggt ggatccggcg gaggtgggtc gggtggcggc 2040
ggatctgaca ttgtgctcac ccaatctcca gcttctttgg ctgtgtctct aggtcagaga
2100 gccaccatct cctgcagagc cagtgaaagt gttgaatatt atgtcacaag
tttaatgcag 2160 tggtaccaac agaaaccagg acagccaccc aaactcctca
tctctgctgc tagcaacgta 2220 gaatctgggg tccctgccag gtttagtggc
agtgggtctg ggacagactt tagcctcaac 2280 atccatcctg tggaggagga
tgatattgca atgtatttct gtcagcaaag taggaaggtt 2340 ccatggacgt
tcggtggagg caccaagctg gaaatcaaac gttaatctag a 2391 <210> SEQ
ID NO 180 <211> LENGTH: 790 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-STD2-2e12HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(537) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (538)..(658) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (659)..(678) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (679)..(790) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 180 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr Gly Gly
Gly Gly Ser Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln Leu Lys Glu Ser 530 535 540 Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 545 550 555 560 Val Ser
Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 565 570 575
Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly 580
585 590 Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys 595 600 605 Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr 610 615 620 Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser Asn Phe 625 630 635 640 His Tyr Tyr Val Met Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val 645 650 655 Ser Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 660 665 670 Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 675 680 685 Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser 690 695 700
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln 705
710 715 720 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser
Asn Val 725 730 735 Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly
Ser Gly Thr Asp 740 745 750 Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp Ile Ala Met Tyr 755 760 765 Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe Gly Gly Gly Thr 770 775 780 Lys Leu Glu Ile Lys Arg
785 790 <210> SEQ ID NO 181 <211> LENGTH: 790
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1(P238S/P331S)-STD2-2e12HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(537) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (538)..(658) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (659)..(678) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (679)..(790) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 181 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Ser Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr Gly Gly
Gly Gly Ser Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln Leu Lys Glu Ser 530 535 540 Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 545 550 555 560 Val Ser
Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 565 570 575
Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly 580
585 590 Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys 595 600 605 Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr 610 615 620 Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser Asn Phe 625 630 635 640 His Tyr Tyr Val Met Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val 645 650 655 Ser Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 660 665 670 Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 675 680 685 Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser 690 695 700
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln 705
710 715 720 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser
Asn Val 725 730 735 Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly
Ser Gly Thr Asp 740 745 750 Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp Ile Ala Met Tyr 755 760 765 Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe Gly Gly Gly Thr 770 775 780 Lys Leu Glu Ile Lys Arg
785 790 <210> SEQ ID NO 182 <211> LENGTH: 2283
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H1-2e12HL (DNA) <400> SEQUENCE: 182 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attctcaggt gcagctgaag gagtcaggac ctggcctggt ggcgccctca
1560 cagagcctgt ccatcacatg caccgtctca gggttctcat taaccggcta
tggtgtaaac 1620 tgggttcgcc agcctccagg aaagggtctg gagtggctgg
gaatgatatg gggtgatgga 1680 agcacagact ataattcagc tctcaaatcc
agactatcga tcaccaagga caactccaag 1740 agccaagttt tcttaaaaat
gaacagtctg caaactgatg acacagccag atactactgt 1800 gctcgagatg
gttatagtaa ctttcattac tatgttatgg actactgggg tcaaggaacc 1860
tcagtcaccg tctcctctgg gggtggaggc tctggtggcg gtggatccgg cggaggtggg
1920 tcgggtggcg gcggatctga cattgtgctc acccaatctc cagcttcttt
ggctgtgtct 1980 ctaggtcaga gagccaccat ctcctgcaga gccagtgaaa
gtgttgaata ttatgtcaca 2040 agtttaatgc agtggtacca acagaaacca
ggacagccac ccaaactcct catctctgct 2100 gctagcaacg tagaatctgg
ggtccctgcc aggtttagtg gcagtgggtc tgggacagac 2160 tttagcctca
acatccatcc tgtggaggag gatgatattg caatgtattt ctgtcagcaa 2220
agtaggaagg ttccatggac gttcggtgga ggcaccaagc tggaaatcaa acgttaatct
2280 aga 2283 <210> SEQ ID NO 183 <211> LENGTH: 754
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H1-2e12HL (w/2e12 leader) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(281) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(501) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (502)..(622) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (623)..(642) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (643)..(754) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 183 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Ser Gln Val Gln Leu Lys Glu
Ser Gly Pro Gly Leu 500 505 510 Val Ala Pro Ser Gln Ser Leu Ser Ile
Thr Cys Thr Val Ser Gly Phe 515 520 525 Ser Leu Thr Gly Tyr Gly Val
Asn Trp Val Arg Gln Pro Pro Gly Lys 530 535 540 Gly Leu Glu Trp Leu
Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr 545 550 555 560 Asn Ser
Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys 565 570 575
Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 580
585 590 Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr
Val 595 600 605 Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser
Ser Gly Gly 610 615 620 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly 625 630 635 640 Gly Ser Asp Ile Val Leu Thr Gln
Ser Pro Ala Ser Leu Ala Val Ser 645 650 655 Leu Gly Gln Arg Ala Thr
Ile Ser Cys Arg Ala Ser Glu Ser Val Glu 660 665 670 Tyr Tyr Val Thr
Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln 675 680 685 Pro Pro
Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val 690 695 700
Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn 705
710 715 720 Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys
Gln Gln 725 730 735 Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr
Lys Leu Glu Ile 740 745 750 Lys Arg <210> SEQ ID NO 184
<211> LENGTH: 2301 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H2-2e12HL (DNA) <400> SEQUENCE:
184 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat
cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc tcccagtctc
cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat gacttgcagg
gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga agccaggatc
ctcccccaaa ccctggattt atgccccatc caacctggct 240 tctggagtcc
ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300
agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca
360 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc
gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct tatctacagc
agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa gatgtcctgc
aaggcttctg gctacacatt taccagttac 540 aatatgcact gggtaaagca
gacacctaga cagggcctgg aatggattgg agctatttat 600 ccaggaaatg
gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660
gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg
720 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt
cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat caggagccca
aatcttctga caaaactcac 840 acatccccac cgagcccagc acctgaactc
ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca aggacaccct
catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 gacgtgagcc
acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc
1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa acaatctcca
aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct gcccccatcc
cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc tggtcaaagg
cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320 gggcagccgg
agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380
ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca
1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct
ctccctgtct 1500 ccgggtaagg gtggcggtgg ctcggggaat tctcaggtgc
agctgaagga gtcaggacct 1560 ggcctggtgg cgccctcaca gagcctgtcc
atcacatgca ccgtctcagg gttctcatta 1620 accggctatg gtgtaaactg
ggttcgccag cctccaggaa agggtctgga gtggctggga 1680 atgatatggg
gtgatggaag cacagactat aattcagctc tcaaatccag actatcgatc 1740
accaaggaca actccaagag ccaagttttc ttaaaaatga acagtctgca aactgatgac
1800 acagccagat actactgtgc tcgagatggt tatagtaact ttcattacta
tgttatggac 1860 tactggggtc aaggaacctc agtcaccgtc tcctctgggg
gtggaggctc tggtggcggt 1920 ggatccggcg gaggtgggtc gggtggcggc
ggatctgaca ttgtgctcac ccaatctcca 1980 gcttctttgg ctgtgtctct
aggtcagaga gccaccatct cctgcagagc cagtgaaagt 2040 gttgaatatt
atgtcacaag tttaatgcag tggtaccaac agaaaccagg acagccaccc 2100
aaactcctca tctctgctgc tagcaacgta gaatctgggg tccctgccag gtttagtggc
2160 agtgggtctg ggacagactt tagcctcaac atccatcctg tggaggagga
tgatattgca 2220 atgtatttct gtcagcaaag taggaaggtt ccatggacgt
tcggtggagg caccaagctg 2280 gaaatcaaac gttaatctag a 2301 <210>
SEQ ID NO 185 <211> LENGTH: 760 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-H2-2e12HL (w/2e12 leader)
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(22) <223> OTHER INFORMATION:
Leader <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (23)..(127) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (128)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(507) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (508)..(628) <223> OTHER INFORMATION:
VH2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (629)..(648) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (649)..(760) <223> OTHER INFORMATION:
VL2 <400> SEQUENCE: 185 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440
445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys
Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln Leu Lys 500 505 510 Glu Ser Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr 515 520 525 Cys Thr Val Ser Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val 530 535 540 Arg Gln Pro
Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly 545 550 555 560
Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile 565
570 575 Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu 580 585 590 Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser 595 600 605 Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly
Gln Gly Thr Ser Val 610 615 620 Thr Val Ser Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Gly Gly 625 630 635 640 Gly Gly Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln Ser Pro 645 650 655 Ala Ser Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg 660 665 670 Ala Ser
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr 675 680 685
Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser 690
695 700 Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser
Gly 705 710 715 720 Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp Ile Ala 725 730 735 Met Tyr Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe Gly Gly 740 745 750 Gly Thr Lys Leu Glu Ile Lys Arg
755 760 <210> SEQ ID NO 186 <211> LENGTH: 2307
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H3-2e12HL (DNA) <400> SEQUENCE: 186 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attatggtgg cggtggctct gggaattctc aggtgcagct gaaggagtca
1560 ggacctggcc tggtggcgcc ctcacagagc ctgtccatca catgcaccgt
ctcagggttc 1620 tcattaaccg gctatggtgt aaactgggtt cgccagcctc
caggaaaggg tctggagtgg 1680 ctgggaatga tatggggtga tggaagcaca
gactataatt cagctctcaa atccagacta 1740 tcgatcacca aggacaactc
caagagccaa gttttcttaa aaatgaacag tctgcaaact 1800 gatgacacag
ccagatacta ctgtgctcga gatggttata gtaactttca ttactatgtt 1860
atggactact ggggtcaagg aacctcagtc accgtctcct ctgggggtgg aggctctggt
1920 ggcggtggat ccggcggagg tgggtcgggt ggcggcggat ctgacattgt
gctcacccaa 1980 tctccagctt ctttggctgt gtctctaggt cagagagcca
ccatctcctg cagagccagt 2040 gaaagtgttg aatattatgt cacaagttta
atgcagtggt accaacagaa accaggacag 2100 ccacccaaac tcctcatctc
tgctgctagc aacgtagaat ctggggtccc tgccaggttt 2160 agtggcagtg
ggtctgggac agactttagc ctcaacatcc atcctgtgga ggaggatgat 2220
attgcaatgt atttctgtca gcaaagtagg aaggttccat ggacgttcgg tggaggcacc
2280 aagctggaaa tcaaacgtta atctaga 2307 <210> SEQ ID NO 187
<211> LENGTH: 762 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: 2H7sssIgG1-H3-2e12HL (w/2e12 leader) (AA) <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(22) <223> OTHER INFORMATION: Leader <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(509) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (510)..(630) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (631)..(650) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (651)..(762) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 187 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln 500 505 510 Leu Lys Glu Ser Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser 515 520 525 Ile Thr Cys Thr Val Ser Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn 530 535 540 Trp Val Arg Gln Pro
Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 545 550 555 560 Trp Gly
Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu 565 570 575
Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 580
585 590 Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly 595 600 605 Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly
Gln Gly Thr 610 615 620 Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln 645 650 655 Ser Pro Ala Ser Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser 660 665 670 Cys Arg Ala Ser
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln 675 680 685 Trp Tyr
Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala 690 695 700
Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly 705
710 715 720 Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp 725 730 735 Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe 740 745 750 Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg
755 760 <210> SEQ ID NO 188 <211> LENGTH: 2316
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H4-2e12HL (DNA) <400> SEQUENCE: 188 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaagg gtggcggtgg ctcgggcggt ggtggatctg ggaattctca ggtgcagctg
1560 aaggagtcag gacctggcct ggtggcgccc tcacagagcc tgtccatcac
atgcaccgtc 1620 tcagggttct cattaaccgg ctatggtgta aactgggttc
gccagcctcc aggaaagggt 1680 ctggagtggc tgggaatgat atggggtgat
ggaagcacag actataattc agctctcaaa 1740 tccagactat cgatcaccaa
ggacaactcc aagagccaag ttttcttaaa aatgaacagt 1800 ctgcaaactg
atgacacagc cagatactac tgtgctcgag atggttatag taactttcat 1860
tactatgtta tggactactg gggtcaagga acctcagtca ccgtctcctc tgggggtgga
1920 ggctctggtg gcggtggatc cggcggaggt gggtcgggtg gcggcggatc
tgacattgtg 1980 ctcacccaat ctccagcttc tttggctgtg tctctaggtc
agagagccac catctcctgc 2040 agagccagtg aaagtgttga atattatgtc
acaagtttaa tgcagtggta ccaacagaaa 2100 ccaggacagc cacccaaact
cctcatctct gctgctagca acgtagaatc tggggtccct 2160 gccaggttta
gtggcagtgg gtctgggaca gactttagcc tcaacatcca tcctgtggag 2220
gaggatgata ttgcaatgta tttctgtcag caaagtagga aggttccatg gacgttcggt
2280 ggaggcacca agctggaaat caaacgttaa tctaga 2316 <210> SEQ
ID NO 189 <211> LENGTH: 765 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H4-2e12HL (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(512) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (513)..(633) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (634)..(653) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (654)..(765) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 189 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Asn Ser 500 505 510 Gln Val Gln Leu Lys Glu Ser Gly Pro
Gly Leu Val Ala Pro Ser Gln 515 520 525 Ser Leu Ser Ile Thr Cys Thr
Val Ser Gly Phe Ser Leu Thr Gly Tyr 530 535 540 Gly Val Asn Trp Val
Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 545 550 555 560 Gly Met
Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys 565 570 575
Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 580
585 590 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys
Ala 595 600 605 Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp
Tyr Trp Gly 610 615 620 Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly
Gly Gly Ser Gly Gly 625 630 635 640 Gly Gly Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Asp Ile Val 645 650 655 Leu Thr Gln Ser Pro Ala
Ser Leu Ala Val Ser Leu Gly Gln Arg Ala 660 665 670 Thr Ile Ser Cys
Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser 675 680 685 Leu Met
Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 690 695 700
Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser 705
710 715 720 Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro
Val Glu 725 730 735 Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser
Arg Lys Val Pro 740 745 750 Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu
Ile Lys Arg 755 760 765 <210> SEQ ID NO 190 <211>
LENGTH: 2322 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H5-2e12HL (DNA) <400> SEQUENCE: 190 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctgggaa ttctcaggtg
1560 cagctgaagg agtcaggacc tggcctggtg gcgccctcac agagcctgtc
catcacatgc 1620 accgtctcag ggttctcatt aaccggctat ggtgtaaact
gggttcgcca gcctccagga 1680 aagggtctgg agtggctggg aatgatatgg
ggtgatggaa gcacagacta taattcagct 1740 ctcaaatcca gactatcgat
caccaaggac aactccaaga gccaagtttt cttaaaaatg 1800 aacagtctgc
aaactgatga cacagccaga tactactgtg ctcgagatgg ttatagtaac 1860
tttcattact atgttatgga ctactggggt caaggaacct cagtcaccgt ctcctctggg
1920 ggtggaggct ctggtggcgg tggatccggc ggaggtgggt cgggtggcgg
cggatctgac 1980 attgtgctca cccaatctcc agcttctttg gctgtgtctc
taggtcagag agccaccatc 2040 tcctgcagag ccagtgaaag tgttgaatat
tatgtcacaa gtttaatgca gtggtaccaa 2100 cagaaaccag gacagccacc
caaactcctc atctctgctg ctagcaacgt agaatctggg 2160 gtccctgcca
ggtttagtgg cagtgggtct gggacagact ttagcctcaa catccatcct 2220
gtggaggagg atgatattgc aatgtatttc tgtcagcaaa gtaggaaggt tccatggacg
2280 ttcggtggag gcaccaagct ggaaatcaaa cgttaatcta ga 2322
<210> SEQ ID NO 191 <211> LENGTH: 767 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-H5-2e12HL (w/2e12 leader)
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(22) <223> OTHER INFORMATION:
Leader <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (23)..(128) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (129)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(514) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (515)..(635) <223> OTHER INFORMATION:
VH2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (636)..(655) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (656)..(767) <223> OTHER INFORMATION:
VL2 <400> SEQUENCE: 191 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440
445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys
Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly 500 505 510 Asn Ser Gln Val Gln Leu Lys
Glu Ser Gly Pro Gly Leu Val Ala Pro 515 520 525 Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 530 535 540 Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 545 550 555 560
Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 565
570 575 Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln
Val 580 585 590 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala
Arg Tyr Tyr 595 600 605 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
Tyr Val Met Asp Tyr 610 615 620 Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 645 650 655 Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 660 665 670 Arg Ala
Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 675 680 685
Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 690
695 700 Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala
Arg 705 710 715 720 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu
Asn Ile His Pro 725 730 735 Val Glu Glu Asp Asp Ile Ala Met Tyr Phe
Cys Gln Gln Ser Arg Lys 740 745 750 Val Pro Trp Thr Phe Gly Gly Gly
Thr Lys Leu Glu Ile Lys Arg 755 760 765 <210> SEQ ID NO 192
<211> LENGTH: 2331 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H6-2e12HL (DNA) <400> SEQUENCE:
192 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat
cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc tcccagtctc
cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat gacttgcagg
gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga agccaggatc
ctcccccaaa ccctggattt atgccccatc caacctggct 240 tctggagtcc
ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300
agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca
360 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc
gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct tatctacagc
agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa gatgtcctgc
aaggcttctg gctacacatt taccagttac 540 aatatgcact gggtaaagca
gacacctaga cagggcctgg aatggattgg agctatttat 600 ccaggaaatg
gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660
gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg
720 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt
cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat caggagccca
aatcttctga caaaactcac 840 acatccccac cgagcccagc acctgaactc
ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca aggacaccct
catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 gacgtgagcc
acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc
1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa acaatctcca
aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct gcccccatcc
cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc tggtcaaagg
cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320 gggcagccgg
agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380
ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca
1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct
ctccctgtct 1500 ccgggtaagg gtggcggtgg ctcgggcggt ggtggatctg
ggggaggagg cagcgggaat 1560 tctcaggtgc agctgaagga gtcaggacct
ggcctggtgg cgccctcaca gagcctgtcc 1620 atcacatgca ccgtctcagg
gttctcatta accggctatg gtgtaaactg ggttcgccag 1680 cctccaggaa
agggtctgga gtggctggga atgatatggg gtgatggaag cacagactat 1740
aattcagctc tcaaatccag actatcgatc accaaggaca actccaagag ccaagttttc
1800 ttaaaaatga acagtctgca aactgatgac acagccagat actactgtgc
tcgagatggt 1860 tatagtaact ttcattacta tgttatggac tactggggtc
aaggaacctc agtcaccgtc 1920 tcctctgggg gtggaggctc tggtggcggt
ggatccggcg gaggtgggtc gggtggcggc 1980 ggatctgaca ttgtgctcac
ccaatctcca gcttctttgg ctgtgtctct aggtcagaga 2040 gccaccatct
cctgcagagc cagtgaaagt gttgaatatt atgtcacaag tttaatgcag 2100
tggtaccaac agaaaccagg acagccaccc aaactcctca tctctgctgc tagcaacgta
2160 gaatctgggg tccctgccag gtttagtggc agtgggtctg ggacagactt
tagcctcaac 2220 atccatcctg tggaggagga tgatattgca atgtatttct
gtcagcaaag taggaaggtt 2280 ccatggacgt tcggtggagg caccaagctg
gaaatcaaac gttaatctag a 2331 <210> SEQ ID NO 193 <211>
LENGTH: 770 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H6-2e12HL (w/2e12 leader) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(517) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (518)..(638) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (639)..(658) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (659)..(770) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 193 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly 500 505 510 Gly Ser Gly Asn Ser Gln Val Gln Leu
Lys Glu Ser Gly Pro Gly Leu 515 520 525 Val Ala Pro Ser Gln Ser Leu
Ser Ile Thr Cys Thr Val Ser Gly Phe 530 535 540 Ser Leu Thr Gly Tyr
Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys 545 550 555 560 Gly Leu
Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr 565 570 575
Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys 580
585 590 Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr
Ala 595 600 605 Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His
Tyr Tyr Val 610 615 620 Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr
Val Ser Ser Gly Gly 625 630 635 640 Gly Gly Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Gly Gly Gly 645 650 655 Gly Ser Asp Ile Val Leu
Thr Gln Ser Pro Ala Ser Leu Ala Val Ser 660 665 670 Leu Gly Gln Arg
Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu 675 680 685 Tyr Tyr
Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln 690 695 700
Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val 705
710 715 720 Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser
Leu Asn 725 730 735 Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr
Phe Cys Gln Gln 740 745 750 Ser Arg Lys Val Pro Trp Thr Phe Gly Gly
Gly Thr Lys Leu Glu Ile 755 760 765 Lys Arg 770 <210> SEQ ID
NO 194 <211> LENGTH: 2301 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sscIgG1-H7-2e12HL (DNA) <400> SEQUENCE:
194 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat
cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc tcccagtctc
cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat gacttgcagg
gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga agccaggatc
ctcccccaaa ccctggattt atgccccatc caacctggct 240 tctggagtcc
ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300
agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca
360 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc
gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct tatctacagc
agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa gatgtcctgc
aaggcttctg gctacacatt taccagttac 540 aatatgcact gggtaaagca
gacacctaga cagggcctgg aatggattgg agctatttat 600 ccaggaaatg
gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660
gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg
720 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt
cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat caggagccca
aatcttctga caaaactcac 840 acatccccac cgtgcccagc acctgaactc
ctggggggat cgtcagtctt cctcttcccc 900 ccaaaaccca aggacaccct
catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 gacgtgagcc
acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc
1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa accatctcca
aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct gcccccatcc
cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc tggtcaaagg
cttctatcca agcgacatcg ccgtggagtg ggagagcaat 1320 gggcagccgg
agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380
ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca
1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct
ctccctgtct 1500 ccgggtaagg ggtgtccacc ttgtccgaat tctcaggtgc
agctgaagga gtcagggcct 1560 ggcagcgtgg cgccctcaca gagcctgtcc
atcacatgca ccgtctcagg gttctcatta 1620 accggctatg gtgtaaactg
ggttcgccag cctccaggaa agggtctgga gtggctggga 1680 atgatatggg
gtgatggaag cacagactat aattcagctc tcaaatccag actatcgatc 1740
accaaggaca actccaagag ccaagttttc ttaaaaatga acagtctgca aactgatgac
1800 acagccagat actactgtgc tcgagatggt tatagtaact ttcattacta
tgttatggac 1860 tactggggtc aaggaacctc agtcaccgtc tcctctgggg
gtggaggctc tggtggcggt 1920 ggatccggcg gaggtgggtc gggtggcggc
ggatctgaca ttgtgctcac ccaatctcca 1980 gcttctttgg ctgtgtctct
aggtcagaga gccaccatct cctgcagagc cagtgaaagt 2040 gttgaatatt
atgtcacaag tttaatgcag tggtaccaac agaaaccagg acagccaccc 2100
aagctcctca tctctgctgc tagcaacgta gaatctgggg tccctgccag gtttagtggc
2160 agtgggtctg ggacagactt tagcctcaac atccatcctg tggaggagga
tgatattgca 2220 atgtatttct gtcagcaaag taggaaggtt ccatggacgt
tcggtggagg caccaagctg 2280 gaaatcaaac gttaatctag a 2301 <210>
SEQ ID NO 195 <211> LENGTH: 760 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sscIgG1-H7-2e12HL (w/2e12 linker
) (AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(22) <223> OTHER INFORMATION:
Leader <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (23)..(128) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (129)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(507) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (508)..(628) <223> OTHER INFORMATION:
VH2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (629)..(648) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (649)..(760) <223> OTHER INFORMATION:
VL2 <400> SEQUENCE: 195 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440
445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys
Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Gly Cys Pro Pro Cys Pro
Asn Ser Gln Val Gln Leu Lys 500 505 510 Glu Ser Gly Pro Gly Ser Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr 515 520 525 Cys Thr Val Ser Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val 530 535 540 Arg Gln Pro
Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly 545 550 555 560
Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile 565
570 575 Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu 580 585 590 Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser 595 600 605 Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly
Gln Gly Thr Ser Val 610 615 620 Thr Val Ser Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Gly Gly 625 630 635 640 Gly Gly Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln Ser Pro 645 650 655 Ala Ser Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg 660 665 670 Ala Ser
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr 675 680 685
Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser 690
695 700 Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser
Gly 705 710 715 720 Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp Ile Ala 725 730 735 Met Tyr Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe Gly Gly 740 745 750 Gly Thr Lys Leu Glu Ile Lys Arg
755 760 <210> SEQ ID NO 196 <211> LENGTH: 2283
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H7-G194 HL (DNA) <400> SEQUENCE: 196 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgtgcccagc acctgaactc ctggggggat cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatcca agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaagg ggtgtccacc ttgtccgaat tctgaggtcc agctgcaaca gtctggacct
1560 gaactggtga agcctggagc ttcaatgaag atttcctgca aggcctctgg
ttactcattc 1620 actggctaca tcgtgaactg gctgaagcag agccatggaa
agaaccttga gtggattgga 1680 cttattaatc catacaaagg tcttactacc
tacaaccaga aattcaaggg caaggccaca 1740 ttaactgtag acaagtcatc
cagcacagcc tacatggagc tcctcagtct gacatctgaa 1800 gactctgcag
tctattactg tgcaagatct gggtactatg gtgactcgga ctggtacttc 1860
gatgtctggg gcgcagggac cacggtcacc gtctcctctg gtggcggtgg ctcgggcggt
1920 ggtggatctg gaggaggtgg gagcgctagc gacatccaga tgacacagac
tacatcctcc 1980 ctgtctgcct ctctgggaga cagagtcacc atcagttgca
gggcaagtca ggacattcgc 2040 aattatttaa actggtatca gcagaaacca
gatggaactg ttaaactcct gatctactac 2100 acatcaagat tacactcagg
agtcccatca aggttcagtg gcagtgggtc tggaacagat 2160 tattctctca
ccattgccaa cctgcaacca gaagatattg ccacttactt ttgccaacag 2220
ggtaatacgc ttccgtggac gttcggtgga ggcaccaaac tggtaaccaa acggtaatct
2280 aga 2283 <210> SEQ ID NO 197 <211> LENGTH: 754
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H7-G194 HL (w/2e12 leader) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH1 <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(507) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (508)..(629) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (630)..(646) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (647)..(754) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 197 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Cys Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser
Glu Val Gln Leu Gln 500 505 510 Gln Ser Gly Pro Glu Leu Val Lys Pro
Gly Ala Ser Met Lys Ile Ser 515 520 525 Cys Lys Ala Ser Gly Tyr Ser
Phe Thr Gly Tyr Ile Val Asn Trp Leu 530 535 540 Lys Gln Ser His Gly
Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro 545 550 555 560 Tyr Lys
Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr 565 570 575
Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser 580
585 590 Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Gly
Tyr 595 600 605 Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala
Gly Thr Thr 610 615 620 Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 625 630 635 640 Gly Gly Gly Ser Ala Ser Asp Ile
Gln Met Thr Gln Thr Thr Ser Ser 645 650 655 Leu Ser Ala Ser Leu Gly
Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 660 665 670 Gln Asp Ile Arg
Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 675 680 685 Thr Val
Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu His Ser Gly Val 690 695 700
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 705
710 715 720 Ile Ala Asn Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys
Gln Gln 725 730 735 Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly Gly Thr
Lys Leu Val Thr 740 745 750 Lys Arg <210> SEQ ID NO 198
<211> LENGTH: 2298 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H7-G281 HL (DNA) <400>
SEQUENCE: 198 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgtgcccagc
acctgaactc ctggggggat cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
accatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatcca agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaagg ggtgtccacc ttgtccgaat
tctgaggtcc agctgcaaca gtctggacct 1560 gaactggtga agcctggagc
ttcaatgaag atttcctgca aggcctctgg ttactcattc 1620 actggctaca
tcgtgaactg gctgaagcag agccatggaa agaaccttga gtggattgga 1680
cttattaatc catacaaagg tcttactacc tacaaccaga aattcaaggg caaggccaca
1740 ttaactgtag acaagtcatc cagcacagcc tacatggagc tcctcagtct
gacatctgaa 1800 gactctgcag tctattactg tgcaagatct gggtactatg
gtgactcgga ctggtacttc 1860 gatgtctggg gcgcagggac cacggtcacc
gtctcctctg gtggcggtgg ctcgggcggt 1920 ggtggatctg gaggaggtgg
gagcggggga ggtggcagtg ctagcgacat ccagatgaca 1980 cagactacat
cctccctgtc tgcctctctg ggagacagag tcaccatcag ttgcagggca 2040
agtcaggaca ttcgcaatta tttaaactgg tatcagcaga aaccagatgg aactgttaaa
2100 ctcctgatct actacacatc aagattacac tcaggagtcc catcaaggtt
cagtggcagt 2160 gggtctggaa cagattattc tctcaccatt gccaacctgc
aaccagaaga tattgccact 2220 tacttttgcc aacagggtaa tacgcttccg
tggacgttcg gtggaggcac caaactggta 2280 accaaacggt aatctaga 2298
<210> SEQ ID NO 199 <211> LENGTH: 759 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-H7-G281 HL (w/2e12
leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL1 <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH1 <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(282) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(507) <223> OTHER
INFORMATION: EFD-BD2 Linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (508)..(629)
<223> OTHER INFORMATION: VH2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (630)..(651)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(652)..(759) <223> OTHER INFORMATION: VL2 <400>
SEQUENCE: 199 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser Glu Val Gln
Leu Gln 500 505 510 Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser
Met Lys Ile Ser 515 520 525 Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly
Tyr Ile Val Asn Trp Leu 530 535 540 Lys Gln Ser His Gly Lys Asn Leu
Glu Trp Ile Gly Leu Ile Asn Pro 545 550 555 560 Tyr Lys Gly Leu Thr
Thr Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr 565 570 575 Leu Thr Val
Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser 580 585 590 Leu
Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr 595 600
605 Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr
610 615 620 Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly 625 630 635 640 Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser
Asp Ile Gln Met Thr 645 650 655 Gln Thr Thr Ser Ser Leu Ser Ala Ser
Leu Gly Asp Arg Val Thr Ile 660 665 670 Ser Cys Arg Ala Ser Gln Asp
Ile Arg Asn Tyr Leu Asn Trp Tyr Gln 675 680 685 Gln Lys Pro Asp Gly
Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Arg 690 695 700 Leu His Ser
Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 705 710 715 720
Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu Asp Ile Ala Thr 725
730 735 Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly
Gly 740 745 750 Thr Lys Leu Val Thr Lys Arg 755 <210> SEQ ID
NO 200 <211> LENGTH: 1533 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2e12-sss-IgG1 HL SMIP (DNA) <400>
SEQUENCE: 200 atggattttc aagtgcagat tttcagcttc ctgctaatca
gtgcttcagt cataatgtcc 60 agaggagtcc aggtgcagct gaaggagtca
ggacctggcc tggtggcgcc ctcacagagc 120 ctgtccatca catgcaccgt
ctcagggttc tcattaaccg gctatggtgt aaactgggtt 180 cgccagcctc
caggaaaggg tctagagtgg ctgggaatga tatggggtga tggaagcaca 240
gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc caagagccaa
300 gttttcttaa aaatgaacag tctgcaaact gatgacacag ccagatacta
ctgtgctcga 360 gatggttata gtaactttca ttactatgtt atggactact
ggggtcaagg aacctcagtc 420 accgtctcct ctgggggtgg aggctctggt
ggcggtggat ccggcggagg tgggtcgggt 480 ggcggcggat ctgacattgt
gctcacccaa tctccagctt ctttggctgt gtctctaggt 540 cagagagcca
ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta 600
atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc tgctgctagc
660 aacgtagaat ctggggtccc tgccaggttt agtggcagtg ggtctgggac
agactttagc 720 ctcaacatcc atcctgtgga ggaggatgat attgcaatgt
atttctgtca gcaaagtagg 780 aaggttccat ggacgttcgg tggaggcacc
aagctggaaa tcaaacgtga tcaggagccc 840 aaatcttctg acaaaactca
cacatcccca ccgtccccag cacctgaact cctgggggga 900 tcgtcagtct
tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct 960
gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg
1020 tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga
gcagtacaac 1080 agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc
aggactggct gaatggcaag 1140 gagtacaagt gcaaggtctc caacaaagcc
ctcccagcct ccatcgagaa aacaatctcc 1200 aaagccaaag ggcagccccg
agaaccacag gtgtacaccc tgcccccatc ccgggatgag 1260 ctgaccaaga
accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc 1320
gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg
1380 ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa
gagcaggtgg 1440 cagcagggga acgtcttctc atgctccgtg atgcatgagg
ctctgcacaa ccactacacg 1500 cagaagagcc tctccctgtc tccgggtaaa tga
1533 <210> SEQ ID NO 201 <211> LENGTH: 510 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2e12-sss-IgG1 HL SMIP (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (24)..(144) <223> OTHER INFORMATION: VH <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(164) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(165)..(276) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(279)..(293) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 201 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Val Gln Val Gln Leu
Lys Glu Ser Gly Pro 20 25 30 Gly Leu Val Ala Pro Ser Gln Ser Leu
Ser Ile Thr Cys Thr Val Ser 35 40 45 Gly Phe Ser Leu Thr Gly Tyr
Gly Val Asn Trp Val Arg Gln Pro Pro 50 55 60 Gly Lys Gly Leu Glu
Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 65 70 75 80 Asp Tyr Asn
Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 85 90 95 Ser
Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 100 105
110 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
115 120 125 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 130 135 140 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
Gly Gly Ser Gly 145 150 155 160 Gly Gly Gly Ser Asp Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala 165 170 175 Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala Ser Glu Ser 180 185 190 Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 195 200 205 Gly Gln Pro
Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 210 215 220 Gly
Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 225 230
235 240 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe
Cys 245 250 255 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly
Thr Lys Leu 260 265 270 Glu Ile Lys Arg Asp Gln Glu Pro Lys Ser Ser
Asp Lys Thr His Thr 275 280 285 Ser Pro Pro Ser Pro Ala Pro Glu Leu
Leu Gly Gly Ser Ser Val Phe 290 295 300 Leu Phe Pro Pro Lys Pro Lys
Asp Thr Leu Met Ile Ser Arg Thr Pro 305 310 315 320 Glu Val Thr Cys
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 325 330 335 Lys Phe
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 340 345 350
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 355
360 365 Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys
Cys 370 375 380 Lys Val Ser Asn Lys Ala Leu Pro Ala Ser Ile Glu Lys
Thr Ile Ser 385 390 395 400 Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
Val Tyr Thr Leu Pro Pro 405 410 415 Ser Arg Asp Glu Leu Thr Lys Asn
Gln Val Ser Leu Thr Cys Leu Val 420 425 430 Lys Gly Phe Tyr Pro Ser
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 435 440 445 Gln Pro Glu Asn
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 450 455 460 Gly Ser
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 465 470 475
480 Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
485 490 495 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
500 505 510 <210> SEQ ID NO 202 <211> LENGTH: 1518
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: 2e12-sss-IgG1
LH SMIP (DNA) <400> SEQUENCE: 202 atggattttc aagtgcagat
tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60 agaggagtcg
acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 120
agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg
180 cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc
tgctagcaac 240 gtagaatctg gggtccctgc caggtttagt ggcagtgggt
ctgggacaga ctttagcctc 300 aacatccatc ctgtggagga ggatgatatt
gcaatgtatt tctgtcagca aagtaggaag 360 gttccatgga cgttcggtgg
aggcaccaag ctggaaatca aacggggtgg cggtggatcc 420 ggcggaggtg
ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 480
ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc
540 ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctagagtg
gctgggaatg 600 atatggggtg atggaagcac agactataat tcagctctca
aatccagact atcgatcacc 660 aaggacaact ccaagagcca agttttctta
aaaatgaaca gtctgcaaac tgatgacaca 720 gccagatact actgtgctcg
agatggttat agtaactttc attactatgt tatggactac 780 tggggtcaag
gaacctcagt caccgtctcc tctgatcagg agcccaaatc ttctgacaaa 840
actcacacat ccccaccgtc cccagcacct gaactcctgg ggggaccgtc agtcttcctc
900 ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
cacatgcgtg 960 gtggtggacg tgagccacga agaccctgag gtcaagttca
actggtacgt ggacggcgtg 1020 gaggtgcata atgccaagac aaagccgcgg
gaggagcagt acaacagcac gtaccgtgtg 1080 gtcagcgtcc tcaccgtcct
gcaccaggac tggctgaatg gcaaggagta caagtgcaag 1140 gtctccaaca
aagccctccc agcccccatc gagaaaacaa tctccaaagc caaagggcag 1200
ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag
1260 gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
ggagtgggag 1320 agcaatgggc agccggagaa caactacaag accacgcctc
ccgtgctgga ctccgacggc 1380 tccttcttcc tctacagcaa gctcaccgtg
gacaagagca ggtggcagca ggggaacgtc 1440 ttctcatgct ccgtgatgca
tgaggctctg cacaaccact acacgcagaa gagcctctcc 1500 ctgtctccgg
gtaaatga 1518 <210> SEQ ID NO 203 <211> LENGTH: 505
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: 2e12-sss-IgG1
LH SMIP (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(23) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (24)..(135) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (136)..(150) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (151)..(271) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (274)..(288) <223> OTHER
INFORMATION: Hinge <400> SEQUENCE: 203 Met Asp Phe Gln Val
Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met
Ser Arg Gly Val Asp Ile Val Leu Thr Gln Ser Pro Ala 20 25 30 Ser
Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 35 40
45 Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln
50 55 60 Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala
Ser Asn 65 70 75 80 Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser
Gly Ser Gly Thr 85 90 95 Asp Phe Ser Leu Asn Ile His Pro Val Glu
Glu Asp Asp Ile Ala Met 100 105 110 Tyr Phe Cys Gln Gln Ser Arg Lys
Val Pro Trp Thr Phe Gly Gly Gly 115 120 125 Thr Lys Leu Glu Ile Lys
Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 130 135 140 Ser Gly Gly Gly
Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 145 150 155 160 Leu
Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 165 170
175 Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly
180 185 190 Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser
Thr Asp 195 200 205 Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys Asp Asn Ser 210 215 220 Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr Asp Asp Thr 225 230 235 240 Ala Arg Tyr Tyr Cys Ala Arg
Asp Gly Tyr Ser Asn Phe His Tyr Tyr 245 250 255 Val Met Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp 260 265 270 Gln Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro 275 280 285 Ala
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 290 295
300 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
305 310 315 320 Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe
Asn Trp Tyr 325 330 335 Val Asp Gly Val Glu Val His Asn Ala Lys Thr
Lys Pro Arg Glu Glu 340 345 350 Gln Tyr Asn Ser Thr Tyr Arg Val Val
Ser Val Leu Thr Val Leu His 355 360 365 Gln Asp Trp Leu Asn Gly Lys
Glu Tyr Lys Cys Lys Val Ser Asn Lys 370 375 380 Ala Leu Pro Ala Pro
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 385 390 395 400 Pro Arg
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 405 410 415
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 420
425 430 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
Asn 435 440 445 Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser
Phe Phe Leu 450 455 460 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
Gln Gln Gly Asn Val 465 470 475 480 Phe Ser Cys Ser Val Met His Glu
Ala Leu His Asn His Tyr Thr Gln 485 490 495 Lys Ser Leu Ser Leu Ser
Pro Gly Lys 500 505 <210> SEQ ID NO 204 <211> LENGTH:
1498 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION: G28-1
LH SMIP (DNA) <400> SEQUENCE: 204 aagcttgccg ccatggaagc
accagcgcag cttctcttcc tcctgctact ctggctccca 60 gataccaccg
gtgacatcca gatgactcag tctccagcct ccctatctgc atctgtggga 120
gagactgtca ccatcacatg tcgaacaagt gaaaatgttt acagttattt ggcttggtat
180 cagcagaaac agggaaaatc tcctcagctc ctggtctctt ttgcaaaaac
cttagcagaa 240 ggtgtgccat caaggttcag tggcagtgga tcaggcacac
agttttctct gaagatcagc 300 agcctgcagc ctgaagattc tggaagttat
ttctgtcaac atcattccga taatccgtgg 360 acgttcggtg gaggcaccga
actggagatc aaaggtggcg gtggctcggg cggtggtggg 420 tcgggtggcg
gcggatctgc tagcgcagtc cagctgcagc agtctggacc tgagctggaa 480
aagcctggcg cttcagtgaa gatttcctgc aaggcttctg gttactcatt cactggctac
540 aatatgaact gggtgaagca gaataatgga aagagccttg agtggattgg
aaatattgat 600 ccttattatg gtggtactac ctacaaccgg aagttcaagg
gcaaggccac attgactgta 660 gacaaatcct ccagcacagc ctacatgcag
ctcaagagtc tgacatctga ggactctgca 720 gtctattact gtgcaagatc
ggtcggccct atggactact ggggtcaagg aacctcagtc 780 accgtctcga
gcgagcccaa atcttctgac aaaactcaca catgcccacc gtgcccagca 840
cctgaactcc tgggtggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc
900 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca
cgaagaccct 960 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc
ataatgccaa gacaaagccg 1020 cgggaggagc agtacaacag cacgtaccgt
gtggtcagcg tcctcaccgt cctgcaccag 1080 gactggctga atggcaagga
gtacaagtgc aaggtctcca acaaagccct cccagccccc 1140 atcgagaaaa
ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg 1200
cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc
1260 ttctatccaa gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga
gaacaactac 1320 aagaccacgc ctcccgtgct ggactccgac ggctccttct
tcctctacag caagctcacc 1380 gtggacaaga gcaggtggca gcaggggaac
gtcttctcat gctccgtgat gcatgaggct 1440 ctgcacaacc actacacgca
gaagagcctc tccctgtctc cgggtaagtg actctaga 1498 <210> SEQ ID
NO 205 <211> LENGTH: 492 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: G28-1 LH SMIP (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(20)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(127) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(128)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(260) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(261)..(275) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 205 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser
Pro Ala Ser Leu Ser 20 25 30 Ala Ser Val Gly Glu Thr Val Thr Ile
Thr Cys Arg Thr Ser Glu Asn 35 40 45 Val Tyr Ser Tyr Leu Ala Trp
Tyr Gln Gln Lys Gln Gly Lys Ser Pro 50 55 60 Gln Leu Leu Val Ser
Phe Ala Lys Thr Leu Ala Glu Gly Val Pro Ser 65 70 75 80 Arg Phe Ser
Gly Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser 85 90 95 Ser
Leu Gln Pro Glu Asp Ser Gly Ser Tyr Phe Cys Gln His His Ser 100 105
110 Asp Asn Pro Trp Thr Phe Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly
115 120 125 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Ala Ser 130 135 140 Ala Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu
Lys Pro Gly Ala 145 150 155 160 Ser Val Lys Ile Ser Cys Lys Ala Ser
Gly Tyr Ser Phe Thr Gly Tyr 165 170 175 Asn Met Asn Trp Val Lys Gln
Asn Asn Gly Lys Ser Leu Glu Trp Ile 180 185 190 Gly Asn Ile Asp Pro
Tyr Tyr Gly Gly Thr Thr Tyr Asn Arg Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 225 230
235 240 Ala Arg Ser Val Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val 245 250 255 Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr His
Thr Cys Pro 260 265 270 Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
Ser Val Phe Leu Phe 275 280 285 Pro Pro Lys Pro Lys Asp Thr Leu Met
Ile Ser Arg Thr Pro Glu Val 290 295 300 Thr Cys Val Val Val Asp Val
Ser His Glu Asp Pro Glu Val Lys Phe 305 310 315 320 Asn Trp Tyr Val
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 325 330 335 Arg Glu
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 340 345 350
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 355
360 365 Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
Ala 370 375 380 Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
Pro Ser Arg 385 390 395 400 Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
Thr Cys Leu Val Lys Gly 405 410 415 Phe Tyr Pro Ser Asp Ile Ala Val
Glu Trp Glu Ser Asn Gly Gln Pro 420 425 430 Glu Asn Asn Tyr Lys Thr
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 435 440 445 Phe Phe Leu Tyr
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 450 455 460 Gly Asn
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 465 470 475
480 Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490
<210> SEQ ID NO 206 <211> LENGTH: 1522 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: G28-1 HL SMIP (DNA)
<400> SEQUENCE: 206 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtgcggtcca
gctgcagcag tctggacctg agtcggaaaa gcctggcgct 120 tcagtgaaga
tttcctgcaa ggcttctggt tactcattca ctggctacaa tatgaactgg 180
gtgaagcaga ataatggaaa gagccttgag tggattggaa atattgatcc ttattatggt
240 ggtactacct acaaccggaa gttcaagggc aaggccacat tgactgtaga
caaatcctcc 300 ggcacagcct acatgcagct caagagtctg acatctgagg
actctgcagt ctattactgt 360 gcaagatcgg tcggccctat ggactactgg
ggtcaaggaa cctcagtcac cgtctcttct 420 ggtggcggtg gctcgggcgg
tggtgggtcg ggtggcggcg gatcaggagg aggcgggagt 480 gctagcgaca
tccagatgac tcagtctcca gcctccctat ctgcatctgt gggagagact 540
gtcaccatca catgtcgaac aagtgaaaat gtttacagtt atttggcttg gtatcagcag
600 aaacagggaa aatctcctca gctcctggtc tcttttgcaa aaaccttagc
agaaggtgtg 660 ccatcaaggt tcagtggcag tggatcaggc acacagtttt
ctctgaagat cagcagcctg 720 cagcctgaag attctggaag ttatttctgt
caacatcatt ccgataatcc gtggacgttc 780 ggtggaggca ccgaactgga
gatcaaaggc tcgagcgagc ccaaatcttc tgacaaaact 840 cacacatgcc
caccgtgccc agcacctgaa ctcctgggtg gaccgtcagt cttcctcttc 900
cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg
960 gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga
cggcgtggag 1020 gtgcataatg ccaagacaaa gccgcgggag gagcagtaca
acagcacgta ccgtgtggtc 1080 agcgtcctca ccgtcctgca ccaggactgg
ctgaatggca aggagtacaa gtgcaaggtc 1140 tccaacaaag ccctcccagc
ccccatcgag aaaaccatct ccaaagccaa agggcagccc 1200 cgagaaccac
aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc 1260
agcctgacct gcctggtcaa aggcttctat ccaagcgaca tcgccgtgga gtgggagagc
1320 aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc
cgacggctcc 1380 ttcttcctct acagcaagct caccgtggac aagagcaggt
ggcagcaggg gaacgtcttc 1440 tcatgctccg tgatgcatga ggctctgcac
aaccactaca cgcagaagag cctctccctg 1500 tctccgggta agtgactcta ga 1522
<210> SEQ ID NO 207 <211> LENGTH: 500 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: G28-1 HL SMIP (AA) <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(1)..(20) <223> OTHER INFORMATION: Leader <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(136) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(137)..(158) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(159)..(266) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(283) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 207 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Ala Val Gln Leu Gln Gln Ser
Gly Pro Glu Ser Glu 20 25 30 Lys Pro Gly Ala Ser Val Lys Ile Ser
Cys Lys Ala Ser Gly Tyr Ser 35 40 45 Phe Thr Gly Tyr Asn Met Asn
Trp Val Lys Gln Asn Asn Gly Lys Ser 50 55 60 Leu Glu Trp Ile Gly
Asn Ile Asp Pro Tyr Tyr Gly Gly Thr Thr Tyr 65 70 75 80 Asn Arg Lys
Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser 85 90 95 Gly
Thr Ala Tyr Met Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala 100 105
110 Val Tyr Tyr Cys Ala Arg Ser Val Gly Pro Met Asp Tyr Trp Gly Gln
115 120 125 Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
Gly Gly 130 135 140 Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Ala Ser Asp Ile 145 150 155 160 Gln Met Thr Gln Ser Pro Ala Ser Leu
Ser Ala Ser Val Gly Glu Thr 165 170 175 Val Thr Ile Thr Cys Arg Thr
Ser Glu Asn Val Tyr Ser Tyr Leu Ala 180 185 190 Trp Tyr Gln Gln Lys
Gln Gly Lys Ser Pro Gln Leu Leu Val Ser Phe 195 200 205 Ala Lys Thr
Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 210 215 220 Ser
Gly Thr Gln Phe Ser Leu Lys Ile Ser Ser Leu Gln Pro Glu Asp 225 230
235 240 Ser Gly Ser Tyr Phe Cys Gln His His Ser Asp Asn Pro Trp Thr
Phe 245 250 255 Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly Ser Ser Glu
Pro Lys Ser 260 265 270 Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro
Ala Pro Glu Leu Leu 275 280 285 Gly Gly Pro Ser Val Phe Leu Phe Pro
Pro Lys Pro Lys Asp Thr Leu 290 295 300 Met Ile Ser Arg Thr Pro Glu
Val Thr Cys Val Val Val Asp Val Ser 305 310 315 320 His Glu Asp Pro
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 325 330 335 Val His
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 340 345 350
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 355
360 365 Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
Pro 370 375 380 Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
Glu Pro Gln 385 390 395 400 Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
Leu Thr Lys Asn Gln Val 405 410 415 Ser Leu Thr Cys Leu Val Lys Gly
Phe Tyr Pro Ser Asp Ile Ala Val 420 425 430 Glu Trp Glu Ser Asn Gly
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 435 440 445 Pro Val Leu Asp
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 450 455 460 Val Asp
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 465 470 475
480 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
485 490 495 Ser Pro Gly Lys 500 <210> SEQ ID NO 208
<211> LENGTH: 1522 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: G19-4 LH SMIP (DNA) <400> SEQUENCE: 208
aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca
60 gataccaccg gtgacatcca gatgacacag actacatcct ccctgtctgc
ctctctggga 120 gacagagtca ccatcagttg cagggcaagt caggacattc
gcaattattt aaactggtat 180 cagcagaaac cagatggaac tgttaaactc
ctgatctact acacatcaag attacactca 240 ggagtcccat caaggttcag
tggcagtggg tctggaacag attattctct caccattgcc 300 aacctgcaac
cagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtgg 360
acgttcggtg gaggcaccaa actggtaacc aaacggggtg gcggtggctc gggcggtggt
420 ggatctggag gaggtgggag cgctagcgag gtccagctgc aacagtctgg
acctgaactg 480 gtgaagcctg gagcttcaat gaagatttcc tgcaaggcct
ctggttactc attcactggc 540 tacatcgtga actggctgaa gcagagccat
ggaaagaacc ttgagtggat tggacttatt 600 aatccataca aaggtcttac
tacctacaac cagaaattca agggcaaggc cacattaact 660 gtagacaagt
catccagcac agcctacatg gagctcctca gtctgacatc tgaagactct 720
gcagtctatt actgtgcaag atctgggtac tatggtgact cggactggta cttcgatgtc
780 tggggcgcag ggaccacggt caccgtctcc tcgagcgagc ccaaatcttc
tgacaaaact 840 cacacatgcc caccgtgccc agcacctgaa ctcctgggtg
gaccgtcagt cttcctcttc 900 cccccaaaac ccaaggacac cctcatgatc
tcccggaccc ctgaggtcac atgcgtggtg 960 gtggacgtga gccacgaaga
ccctgaggtc aagttcaact ggtacgtgga cggcgtggag 1020 gtgcataatg
ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc 1080
agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc
1140 tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa
agggcagccc 1200 cgagaaccac aggtgtacac cctgccccca tcccgggatg
agctgaccaa gaaccaggtc 1260 agcctgacct gcctggtcaa aggcttctat
ccaagcgaca tcgccgtgga gtgggagagc 1320 aatgggcagc cggagaacaa
ctacaagacc acgcctcccg tgctggactc cgacggctcc 1380 ttcttcctct
acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc 1440
tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg
1500 tctccgggta agtgactcta ga 1522 <210> SEQ ID NO 209
<211> LENGTH: 500 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: G19-4 LH SMIP (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(128)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (129)..(145)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(146)..(267) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 209 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Thr
Thr Ser Ser Leu Ser 20 25 30 Ala Ser Leu Gly Asp Arg Val Thr Ile
Ser Cys Arg Ala Ser Gln Asp 35 40 45 Ile Arg Asn Tyr Leu Asn Trp
Tyr Gln Gln Lys Pro Asp Gly Thr Val 50 55 60 Lys Leu Leu Ile Tyr
Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser 65 70 75 80 Arg Phe Ser
Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala 85 90 95 Asn
Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn 100 105
110 Thr Leu Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg
115 120 125 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ala 130 135 140 Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu
Val Lys Pro Gly 145 150 155 160 Ala Ser Met Lys Ile Ser Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly 165 170 175 Tyr Ile Val Asn Trp Leu Lys
Gln Ser His Gly Lys Asn Leu Glu Trp 180 185 190 Ile Gly Leu Ile Asn
Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys 195 200 205 Phe Lys Gly
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala 210 215 220 Tyr
Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 225 230
235 240 Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp
Val 245 250 255 Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Ser Glu
Pro Lys Ser 260 265 270 Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro
Ala Pro Glu Leu Leu 275 280 285 Gly Gly Pro Ser Val Phe Leu Phe Pro
Pro Lys Pro Lys Asp Thr Leu 290 295 300 Met Ile Ser Arg Thr Pro Glu
Val Thr Cys Val Val Val Asp Val Ser 305 310 315 320 His Glu Asp Pro
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 325 330 335 Val His
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 340 345 350
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 355
360 365 Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
Pro 370 375 380 Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
Glu Pro Gln 385 390 395 400 Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
Leu Thr Lys Asn Gln Val 405 410 415 Ser Leu Thr Cys Leu Val Lys Gly
Phe Tyr Pro Ser Asp Ile Ala Val 420 425 430 Glu Trp Glu Ser Asn Gly
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 435 440 445 Pro Val Leu Asp
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 450 455 460 Val Asp
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 465 470 475
480 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
485 490 495 Ser Pro Gly Lys 500 <210> SEQ ID NO 210
<211> LENGTH: 1525 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: G19-4 HL SMIP (DNA) <400> SEQUENCE: 210
aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca
60 gataccaccg gtgaggtcca gctgcaacag tctggacctg aactggtgaa
gcctggagct 120 tcaatgaaga tttcctgcaa ggcctctggt tactcattca
ctggctacat cgtgaactgg 180 ctgaagcaga gccatggaaa gaaccttgag
tggattggac ttattaatcc atacaaaggt 240 cttactacct acaaccagaa
attcaagggc aaggccacat tgactgtaga caagtcatcc 300 agcacagcct
acatggagct cctcagtctg acatctgaag actctgcagt ctattactgt 360
gcaagatctg ggtactatgg tgactcagac tggtacttcg atgtctgggg cgcagggacc
420 acagtcaccg tctcctctgg cggcggtggc tcgggcggtg gtggatctgg
aggaggtggg 480 agcgctagcg acatccagat gacacagact acatcctccc
tgtctgcctc tctgggagac 540 agagtcacca tcagttgcag ggcaagtcag
gacattcgca attatttgaa ctggtatcag 600 cagaaaccag atggaactgt
taaactcctg atctactaca catcaagact acactcagga 660 gtcccatcaa
ggttcagtgg cagtgggtct ggaacagatt attctctcac cattgccaac 720
ctgcaaccag aagatattgc cacttacttt tgccaacagg gtaatacact tccctggacg
780 ttcggtggag ggaccaaact ggtgaccaaa cgctcgagcg agcccaaatc
ttctgacaaa 840 actcacacat gcccaccgtg cccagcacct gaactcctgg
gtggaccgtc agtcttcctc 900 ttccccccaa aacccaagga caccctcatg
atctcccgga cccctgaggt cacatgcgtg 960 gtggtggacg tgagccacga
agaccctgag gtcaagttca actggtacgt ggacggcgtg 1020 gaggtgcata
atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg 1080
gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag
1140 gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
caaagggcag 1200 ccccgagaac cacaggtgta caccctgccc ccatcccggg
atgagctgac caagaaccag 1260 gtcagcctga cctgcctggt caaaggcttc
tatccaagcg acatcgccgt ggagtgggag 1320 agcaatgggc agccggagaa
caactacaag accacgcctc ccgtgctgga ctccgacggc 1380 tccttcttcc
tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc 1440
ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc
1500 ctgtctccgg gtaagtgact ctaga 1525 <210> SEQ ID NO 211
<211> LENGTH: 501 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: G19-4 HL SMIP (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(142)
<223> OTHER INFORMATION: VH <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (143)..(159)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(160)..(267) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(270)..(284) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 211 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Glu Val Gln Leu Gln Gln Ser
Gly Pro Glu Leu Val 20 25 30 Lys Pro Gly Ala Ser Met Lys Ile Ser
Cys Lys Ala Ser Gly Tyr Ser 35 40 45 Phe Thr Gly Tyr Ile Val Asn
Trp Leu Lys Gln Ser His Gly Lys Asn 50 55 60 Leu Glu Trp Ile Gly
Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr 65 70 75 80 Asn Gln Lys
Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser 85 90 95 Ser
Thr Ala Tyr Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala 100 105
110 Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr
115 120 125 Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser
Gly Gly 130 135 140 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ala Ser Asp 145 150 155 160 Ile Gln Met Thr Gln Thr Thr Ser Ser
Leu Ser Ala Ser Leu Gly Asp 165 170 175 Arg Val Thr Ile Ser Cys Arg
Ala Ser Gln Asp Ile Arg Asn Tyr Leu 180 185 190 Asn Trp Tyr Gln Gln
Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 195 200 205 Tyr Thr Ser
Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 210 215 220 Gly
Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu 225 230
235 240 Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp
Thr 245 250 255 Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg Ser Ser
Glu Pro Lys 260 265 270 Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys
Pro Ala Pro Glu Leu 275 280 285 Leu Gly Gly Pro Ser Val Phe Leu Phe
Pro Pro Lys Pro Lys Asp Thr 290 295 300 Leu Met Ile Ser Arg Thr Pro
Glu Val Thr Cys Val Val Val Asp Val 305 310 315 320 Ser His Glu Asp
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 325 330 335 Glu Val
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 340 345 350
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 355
360 365 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
Ala 370 375 380 Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
Arg Glu Pro 385 390 395 400 Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
Glu Leu Thr Lys Asn Gln 405 410 415 Val Ser Leu Thr Cys Leu Val Lys
Gly Phe Tyr Pro Ser Asp Ile Ala 420 425 430 Val Glu Trp Glu Ser Asn
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 435 440 445 Pro Pro Val Leu
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 450 455 460 Thr Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 465 470 475
480 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
485 490 495 Leu Ser Pro Gly Lys 500 <210> SEQ ID NO 212
<211> LENGTH: 2328 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: n2H7sssIgG1-STD1-2e12HL (DNA) <400>
SEQUENCE: 212 aagcttgccg ccatggaagc accagcgcag cttctcttcc
tcctgctact ctggctccca 60 gataccaccg gtcaaattgt tctctcccag
tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca caatgacttg
cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180 cagaagccag
gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240
gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga
300 gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa
cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg
gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca ggcttatcta
cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag tgaagatgtc
ctgcaaggct tctggctaca catttaccag ttacaatatg 540 cactgggtaa
agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600
aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa
660 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc
tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt
acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc gagcgagccc
aaatcttctg acaaaactca cacatcccca 840 ccgagcccag cacctgaact
cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900 aaggacaccc
tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960
cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag gagtacaagt
gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa aacaatctcc
aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc tgcccccatc
ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260 ctggtcaaag
gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320
gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac
1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg cagaagagcc
tctccctgtc tccgggtaag 1500 aattatggtg gcggtggctc gggcggtggt
ggatctggag gaggtgggag tgggaattct 1560 caggtgcagc tgaaggagtc
aggacctggc ctggtggcgc cctcacagag cctgtccatc 1620 acatgcaccg
tctcagggtt ctcattaacc ggctatggtg taaactgggt tcgccagcct 1680
ccaggaaagg gtctggagtg gctgggaatg atatggggtg atggaagcac agactataat
1740 tcagctctca aatccagact atcgatcacc aaggacaact ccaagagcca
agttttctta 1800 aaaatgaaca gtctgcaaac tgatgacaca gccagatact
actgtgctcg agatggttat 1860 agtaactttc attactatgt tatggactac
tggggtcaag gaacctcagt caccgtctcc 1920 tctgggggtg gaggctctgg
tggcggtgga tccggcggag gtgggtcggg tggcggcgga 1980 tctgacattg
tgctcaccca atctccagct tctttggctg tgtctctagg tcagagagcc 2040
accatctcct gcagagccag tgaaagtgtt gaatattatg tcacaagttt aatgcagtgg
2100 taccaacaga aaccaggaca gccacccaaa ctcctcatct ctgctgctag
caacgtagaa 2160 tctggggtcc ctgccaggtt tagtggcagt gggtctggga
cagactttag cctcaacatc 2220 catcctgtgg aggaggatga tattgcaatg
tatttctgtc agcaaagtag gaaggttcca 2280 tggacgttcg gtggaggcac
caagctggaa atcaaacgtt aatctaga 2328 <210> SEQ ID NO 213
<211> LENGTH: 769 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: n2H7sssIgG1-STD1-2e12HL (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(20)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(127) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(128)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(516) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (517)..(637) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (638)..(657) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (658)..(769) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 213 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly 500 505 510 Ser Gly Asn Ser Gln Val Gln Leu Lys
Glu Ser Gly Pro Gly Leu Val 515 520 525 Ala Pro Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser Gly Phe Ser 530 535 540 Leu Thr Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly 545 550 555 560 Leu Glu
Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn 565 570 575
Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser 580
585 590 Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala
Arg 595 600 605 Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
Tyr Val Met 610 615 620 Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser Gly Gly Gly 625 630 635 640 Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly Gly Gly Gly 645 650 655 Ser Asp Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala Val Ser Leu 660 665 670 Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr 675 680 685 Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro 690 695 700
Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro 705
710 715 720 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu
Asn Ile 725 730 735 His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe
Cys Gln Gln Ser 740 745 750 Arg Lys Val Pro Trp Thr Phe Gly Gly Gly
Thr Lys Leu Glu Ile Lys 755 760 765 Arg <210> SEQ ID NO 214
<211> LENGTH: 2313 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: n2H7sssIgG1-STD2-2e12LH (DNA) <400>
SEQUENCE: 214 aagcttgccg ccatggaagc accagcgcag cttctcttcc
tcctgctact ctggctccca 60 gataccaccg gtcaaattgt tctctcccag
tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca caatgacttg
cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180 cagaagccag
gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240
gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga
300 gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa
cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg
gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca ggcttatcta
cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag tgaagatgtc
ctgcaaggct tctggctaca catttaccag ttacaatatg 540 cactgggtaa
agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600
aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa
660 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc
tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt
acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc gagcgagccc
aaatcttctg acaaaactca cacatcccca 840 ccgagcccag cacctgaact
cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900 aaggacaccc
tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960
cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag gagtacaagt
gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa aacaatctcc
aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc tgcccccatc
ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260 ctggtcaaag
gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320
gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac
1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg cagaagagcc
tctccctgtc tccgggtaag 1500 aattatggtg gcggtggctc gggcggtggt
ggatctggag gaggtgggag tgggaattct 1560 gacattgtgc tcacccaatc
tccagcttct ttggctgtgt ctctaggtca gagagccacc 1620 atctcctgca
gagccagtga aagtgttgaa tattatgtca caagtttaat gcagtggtac 1680
caacagaaac caggacagcc acccaaactc ctcatctctg ctgctagcaa cgtagaatct
1740 ggggtccctg ccaggtttag tggcagtggg tctgggacag actttagcct
caacatccat 1800 cctgtggagg aggatgatat tgcaatgtat ttctgtcagc
aaagtaggaa ggttccatgg 1860 acgttcggtg gaggcaccaa gctggaaatc
aaacggggtg gcggtggatc cggcggaggt 1920 gggtcgggtg gcggcggatc
tcaggtgcag ctgaaggagt caggacctgg cctggtggcg 1980 ccctcacaga
gcctgtccat cacatgcacc gtctcagggt tctcattaac cggctatggt 2040
gtaaactggg ttcgccagcc tccaggaaag ggtctggagt ggctgggaat gatatggggt
2100 gatggaagca cagactataa ttcagctctc aaatccagac tatcgatcac
caaggacaac 2160 tccaagagcc aagttttctt aaaaatgaac agtctgcaaa
ctgatgacac agccagatac 2220 tactgtgctc gagatggtta tagtaacttt
cattactatg ttatggacta ctggggtcaa 2280 ggaacctcag tcaccgtctc
ctcttaatct aga 2313 <210> SEQ ID NO 215 <211> LENGTH:
764 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-STD2-2e12LH (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(516) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (517)..(628) <223> OTHER INFORMATION: VL2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (629)..(643) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (644)..(764) <223> OTHER INFORMATION: VH2
<400> SEQUENCE: 215 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly 500 505 510 Ser Gly Asn Ser Asp Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala 515 520 525 Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala Ser Glu Ser 530 535 540 Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 545 550 555 560 Gly Gln
Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 565 570 575
Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 580
585 590 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe
Cys 595 600 605 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly
Thr Lys Leu 610 615 620 Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly
Gly Gly Ser Gly Gly 625 630 635 640 Gly Gly Ser Gln Val Gln Leu Lys
Glu Ser Gly Pro Gly Leu Val Ala 645 650 655 Pro Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser Gly Phe Ser Leu 660 665 670 Thr Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu 675 680 685 Glu Trp
Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser 690 695 700
Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln 705
710 715 720 Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala
Arg Tyr 725 730 735 Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
Tyr Val Met Asp 740 745 750 Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 755 760 <210> SEQ ID NO 216 <211> LENGTH: 2048
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H1-2e12HL (DNA) <400> SEQUENCE: 216 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 atgcatgagg
ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500
aattctcagg tgcagctgaa ggagtcagga cctggcctgg tggcgccctc acagagcctg
1560 tccatcacat gcaccgtctc agggttctca ttaaccggct atggtgtaaa
ctgggttcgc 1620 cagcctccag gaaagggtct ggagtggctg ggaatgatat
ggggtgatgg aagcacagac 1680 tataattcag ctctcaaatc cagactatcg
atcaccaagg acaactccaa gagccaagtt 1740 ttcttaaaaa tgaacagtct
gcaaactgat gacacagcca gatactactg tgctcgagat 1800 ggttatagta
actttcatta ctatgttatg gactactggg gtcaaggaac ctcagtcacc 1860
gtctcctctg ggggtggagg ctctggtggc ggtggatccg gcggaggtgg gtcgggtggc
1920 ggcggatctg acattgtgct cacccaatct ccagcttctt tggctgtgtc
tctaggtcag 1980 agagccacca tctcctgcag agccagtgaa agtgttgaat
attatgtcac aagtttaatg 2040 cagtggta 2048 <210> SEQ ID NO 217
<211> LENGTH: 751 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: n2H7sssIgG1-H1-2e12HL (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(20)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(126) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(498) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (499)..(619) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (620)..(639) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (640)..(751) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 217 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro
Gly Leu Val Ala Pro 500 505 510 Ser Gln Ser Leu Ser Ile Thr Cys Thr
Val Ser Gly Phe Ser Leu Thr 515 520 525 Gly Tyr Gly Val Asn Trp Val
Arg Gln Pro Pro Gly Lys Gly Leu Glu 530 535 540 Trp Leu Gly Met Ile
Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 545 550 555 560 Leu Lys
Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val 565 570 575
Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr 580
585 590 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp
Tyr 595 600 605 Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly
Gly Gly Ser 610 615 620 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Asp 625 630 635 640 Ile Val Leu Thr Gln Ser Pro Ala
Ser Leu Ala Val Ser Leu Gly Gln 645 650 655 Arg Ala Thr Ile Ser Cys
Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 660 665 670 Thr Ser Leu Met
Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 675 680 685 Leu Leu
Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg 690 695 700
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro 705
710 715 720 Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser
Arg Lys 725 730 735 Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu
Ile Lys Arg 740 745 750 <210> SEQ ID NO 218 <211>
LENGTH: 2292 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H2-2e12HL (DNA) <400> SEQUENCE: 218 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 atgcatgagg
ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500
ggtggcggtg gctcggggaa ttctcaggtg cagctgaagg agtcaggacc tggcctggtg
1560 gcgccctcac agagcctgtc catcacatgc accgtctcag ggttctcatt
aaccggctat 1620 ggtgtaaact gggttcgcca gcctccagga aagggtctgg
agtggctggg aatgatatgg 1680 ggtgatggaa gcacagacta taattcagct
ctcaaatcca gactatcgat caccaaggac 1740 aactccaaga gccaagtttt
cttaaaaatg aacagtctgc aaactgatga cacagccaga 1800 tactactgtg
ctcgagatgg ttatagtaac tttcattact atgttatgga ctactggggt 1860
caaggaacct cagtcaccgt ctcctctggg ggtggaggct ctggtggcgg tggatccggc
1920 ggaggtgggt cgggtggcgg cggatctgac attgtgctca cccaatctcc
agcttctttg 1980 gctgtgtctc taggtcagag agccaccatc tcctgcagag
ccagtgaaag tgttgaatat 2040 tatgtcacaa gtttaatgca gtggtaccaa
cagaaaccag gacagccacc caaactcctc 2100 atctctgctg ctagcaacgt
agaatctggg gtccctgcca ggtttagtgg cagtgggtct 2160 gggacagact
ttagcctcaa catccatcct gtggaggagg atgatattgc aatgtatttc 2220
tgtcagcaaa gtaggaaggt tccatggacg ttcggtggag gcaccaagct ggaaatcaaa
2280 cgttaatcta ga 2292 <210> SEQ ID NO 219 <211>
LENGTH: 757 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H2-2e12HL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(504) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (505)..(625) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (626)..(645) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (646)..(757) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 219 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln
Leu Lys Glu Ser Gly 500 505 510 Pro Gly Leu Val Ala Pro Ser Gln Ser
Leu Ser Ile Thr Cys Thr Val 515 520 525 Ser Gly Phe Ser Leu Thr Gly
Tyr Gly Val Asn Trp Val Arg Gln Pro 530 535 540 Pro Gly Lys Gly Leu
Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser 545 550 555 560 Thr Asp
Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp 565 570 575
Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 580
585 590 Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe
His 595 600 605 Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val
Thr Val Ser 610 615 620 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Asp Ile Val
Leu Thr Gln Ser Pro Ala Ser Leu 645 650 655 Ala Val Ser Leu Gly Gln
Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu 660 665 670 Ser Val Glu Tyr
Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys 675 680 685 Pro Gly
Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu 690 695 700
Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 705
710 715 720 Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met
Tyr Phe 725 730 735 Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly
Gly Gly Thr Lys 740 745 750 Leu Glu Ile Lys Arg 755 <210> SEQ
ID NO 220 <211> LENGTH: 2298 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: n2H7sssIgG1-H3-2e12HL (DNA)
<400> SEQUENCE: 220 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtcaaattgt
tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca
caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180
cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga
240 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac
aatcagcaga 300 gtggaggctg aagatgctgc cacttattac tgccagcagt
ggagttttaa cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa
gatggcggtg gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca
ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag
tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540
cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga
600 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac
tgtagacaaa 660 tcctccagca cagcctacat gcagctcagc agcctgacat
ctgaagactc tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac
tcttactggt acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc
gagcgagccc aaatcttctg acaaaactca cacatcccca 840 ccgagcccag
cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900
aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
960 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
gcataatgcc 1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc
gtgtggtcag cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag
gagtacaagt gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa
aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc
tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260
ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
1320 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
cttcctctac 1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga
acgtcttctc atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg
cagaagagcc tctccctgtc tccgggtaag 1500 aattatggtg gcggtggctc
tgggaattct caggtgcagc tgaaggagtc aggacctggc 1560 ctggtggcgc
cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc 1620
ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg gctgggaatg
1680 atatggggtg atggaagcac agactataat tcagctctca aatccagact
atcgatcacc 1740 aaggacaact ccaagagcca agttttctta aaaatgaaca
gtctgcaaac tgatgacaca 1800 gccagatact actgtgctcg agatggttat
agtaactttc attactatgt tatggactac 1860 tggggtcaag gaacctcagt
caccgtctcc tctgggggtg gaggctctgg tggcggtgga 1920 tccggcggag
gtgggtcggg tggcggcgga tctgacattg tgctcaccca atctccagct 1980
tctttggctg tgtctctagg tcagagagcc accatctcct gcagagccag tgaaagtgtt
2040 gaatattatg tcacaagttt aatgcagtgg taccaacaga aaccaggaca
gccacccaaa 2100 ctcctcatct ctgctgctag caacgtagaa tctggggtcc
ctgccaggtt tagtggcagt 2160 gggtctggga cagactttag cctcaacatc
catcctgtgg aggaggatga tattgcaatg 2220 tatttctgtc agcaaagtag
gaaggttcca tggacgttcg gtggaggcac caagctggaa 2280 atcaaacgtt
aatctaga 2298 <210> SEQ ID NO 221 <211> LENGTH: 759
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H3-2e12HL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(506) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (507)..(627) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (628)..(647) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (648)..(759) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 221 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser Gln
Val Gln Leu Lys Glu 500 505 510 Ser Gly Pro Gly Leu Val Ala Pro Ser
Gln Ser Leu Ser Ile Thr Cys 515 520 525 Thr Val Ser Gly Phe Ser Leu
Thr Gly Tyr Gly Val Asn Trp Val Arg 530 535 540 Gln Pro Pro Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp 545 550 555 560 Gly Ser
Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr 565 570 575
Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 580
585 590 Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser
Asn 595 600 605 Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr
Ser Val Thr 610 615 620 Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly 625 630 635 640 Gly Ser Gly Gly Gly Gly Ser Asp
Ile Val Leu Thr Gln Ser Pro Ala 645 650 655 Ser Leu Ala Val Ser Leu
Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 660 665 670 Ser Glu Ser Val
Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 675 680 685 Gln Lys
Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 690 695 700
Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 705
710 715 720 Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile
Ala Met 725 730 735 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr
Phe Gly Gly Gly 740 745 750 Thr Lys Leu Glu Ile Lys Arg 755
<210> SEQ ID NO 222 <211> LENGTH: 2307 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: n2H7sssIgG1-H4-2e12HL (DNA)
<400> SEQUENCE: 222 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtcaaattgt
tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca
caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180
cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga
240 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac
aatcagcaga 300 gtggaggctg aagatgctgc cacttattac tgccagcagt
ggagttttaa cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa
gatggcggtg gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca
ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag
tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540
cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga
600 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac
tgtagacaaa 660 tcctccagca cagcctacat gcagctcagc agcctgacat
ctgaagactc tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac
tcttactggt acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc
gagcgagccc aaatcttctg acaaaactca cacatcccca 840 ccgagcccag
cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900
aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
960 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
gcataatgcc 1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc
gtgtggtcag cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag
gagtacaagt gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa
aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc
tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260
ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
1320 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
cttcctctac 1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga
acgtcttctc atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg
cagaagagcc tctccctgtc tccgggtaag 1500 ggtggcggtg gctcgggcgg
tggtggatct gggaattctc aggtgcagct gaaggagtca 1560 ggacctggcc
tggtggcgcc ctcacagagc ctgtccatca catgcaccgt ctcagggttc 1620
tcattaaccg gctatggtgt aaactgggtt cgccagcctc caggaaaggg tctggagtgg
1680 ctgggaatga tatggggtga tggaagcaca gactataatt cagctctcaa
atccagacta 1740 tcgatcacca aggacaactc caagagccaa gttttcttaa
aaatgaacag tctgcaaact 1800 gatgacacag ccagatacta ctgtgctcga
gatggttata gtaactttca ttactatgtt 1860 atggactact ggggtcaagg
aacctcagtc accgtctcct ctgggggtgg aggctctggt 1920 ggcggtggat
ccggcggagg tgggtcgggt ggcggcggat ctgacattgt gctcacccaa 1980
tctccagctt ctttggctgt gtctctaggt cagagagcca ccatctcctg cagagccagt
2040 gaaagtgttg aatattatgt cacaagttta atgcagtggt accaacagaa
accaggacag 2100 ccacccaaac tcctcatctc tgctgctagc aacgtagaat
ctggggtccc tgccaggttt 2160 agtggcagtg ggtctgggac agactttagc
ctcaacatcc atcctgtgga ggaggatgat 2220 attgcaatgt atttctgtca
gcaaagtagg aaggttccat ggacgttcgg tggaggcacc 2280 aagctggaaa
tcaaacgtta atctaga 2307 <210> SEQ ID NO 223 <211>
LENGTH: 762 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H4-2e12HL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(509) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (510)..(630) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (631)..(650) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (651)..(762) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 223 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln 500 505 510 Leu Lys Glu Ser Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser 515 520 525 Ile Thr Cys Thr Val Ser Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn 530 535 540 Trp Val Arg Gln Pro
Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 545 550 555 560 Trp Gly
Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu 565 570 575
Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 580
585 590 Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly 595 600 605 Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly
Gln Gly Thr 610 615 620 Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln 645 650 655 Ser Pro Ala Ser Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser 660 665 670 Cys Arg Ala Ser
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln 675 680 685 Trp Tyr
Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala 690 695 700
Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly 705
710 715 720 Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp 725 730 735 Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe 740 745 750 Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg
755 760 <210> SEQ ID NO 224 <211> LENGTH: 2313
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H5-2e12HL (DNA) <400> SEQUENCE: 224 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 atgcatgagg
ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500
aattatggtg gcggtggctc gggcggtggt ggatctggga attctcaggt gcagctgaag
1560 gagtcaggac ctggcctggt ggcgccctca cagagcctgt ccatcacatg
caccgtctca 1620 gggttctcat taaccggcta tggtgtaaac tgggttcgcc
agcctccagg aaagggtctg 1680 gagtggctgg gaatgatatg gggtgatgga
agcacagact ataattcagc tctcaaatcc 1740 agactatcga tcaccaagga
caactccaag agccaagttt tcttaaaaat gaacagtctg 1800 caaactgatg
acacagccag atactactgt gctcgagatg gttatagtaa ctttcattac 1860
tatgttatgg actactgggg tcaaggaacc tcagtcaccg tctcctctgg gggtggaggc
1920 tctggtggcg gtggatccgg cggaggtggg tcgggtggcg gcggatctga
cattgtgctc 1980 acccaatctc cagcttcttt ggctgtgtct ctaggtcaga
gagccaccat ctcctgcaga 2040 gccagtgaaa gtgttgaata ttatgtcaca
agtttaatgc agtggtacca acagaaacca 2100 ggacagccac ccaaactcct
catctctgct gctagcaacg tagaatctgg ggtccctgcc 2160 aggtttagtg
gcagtgggtc tgggacagac tttagcctca acatccatcc tgtggaggag 2220
gatgatattg caatgtattt ctgtcagcaa agtaggaagg ttccatggac gttcggtgga
2280 ggcaccaagc tggaaatcaa acgttaatct aga 2313 <210> SEQ ID
NO 225 <211> LENGTH: 764 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: n2H7sssIgG1-H5-2e12HL (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(20)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(126) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(511) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (512)..(632) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (633)..(652) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (653)..(764) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 225 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Asn Ser Gln 500 505 510 Val Gln Leu Lys Glu Ser Gly Pro Gly
Leu Val Ala Pro Ser Gln Ser 515 520 525 Leu Ser Ile Thr Cys Thr Val
Ser Gly Phe Ser Leu Thr Gly Tyr Gly 530 535 540 Val Asn Trp Val Arg
Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly 545 550 555 560 Met Ile
Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser 565 570 575
Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 580
585 590 Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala
Arg 595 600 605 Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr
Trp Gly Gln 610 615 620 Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly
Gly Ser Gly Gly Gly 625 630 635 640 Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Asp Ile Val Leu 645 650 655 Thr Gln Ser Pro Ala Ser
Leu Ala Val Ser Leu Gly Gln Arg Ala Thr 660 665 670 Ile Ser Cys Arg
Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu 675 680 685 Met Gln
Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 690 695 700
Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly 705
710 715 720 Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val
Glu Glu 725 730 735 Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg
Lys Val Pro Trp 740 745 750 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
Lys Arg 755 760 <210> SEQ ID NO 226 <211> LENGTH: 2322
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H6-2e12HL (DNA) <400> SEQUENCE: 226 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 atgcatgagg
ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500
ggtggcggtg gctcgggcgg tggtggatct gggggaggag gcagcgggaa ttctcaggtg
1560 cagctgaagg agtcaggacc tggcctggtg gcgccctcac agagcctgtc
catcacatgc 1620 accgtctcag ggttctcatt aaccggctat ggtgtaaact
gggttcgcca gcctccagga 1680 aagggtctgg agtggctggg aatgatatgg
ggtgatggaa gcacagacta taattcagct 1740 ctcaaatcca gactatcgat
caccaaggac aactccaaga gccaagtttt cttaaaaatg 1800 aacagtctgc
aaactgatga cacagccaga tactactgtg ctcgagatgg ttatagtaac 1860
tttcattact atgttatgga ctactggggt caaggaacct cagtcaccgt ctcctctggg
1920 ggtggaggct ctggtggcgg tggatccggc ggaggtgggt cgggtggcgg
cggatctgac 1980 attgtgctca cccaatctcc agcttctttg gctgtgtctc
taggtcagag agccaccatc 2040 tcctgcagag ccagtgaaag tgttgaatat
tatgtcacaa gtttaatgca gtggtaccaa 2100 cagaaaccag gacagccacc
caaactcctc atctctgctg ctagcaacgt agaatctggg 2160 gtccctgcca
ggtttagtgg cagtgggtct gggacagact ttagcctcaa catccatcct 2220
gtggaggagg atgatattgc aatgtatttc tgtcagcaaa gtaggaaggt tccatggacg
2280 ttcggtggag gcaccaagct ggaaatcaaa cgttaatcta ga 2322
<210> SEQ ID NO 227 <211> LENGTH: 767 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: n2H7sssIgG1-H6-2e12HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(20) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (21)..(126) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(514) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (515)..(635) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (636)..(655) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (656)..(767) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 227 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Asn Ser Gln Val Gln Leu Lys Glu Ser
Gly Pro Gly Leu Val Ala Pro 515 520 525 Ser Gln Ser Leu Ser Ile Thr
Cys Thr Val Ser Gly Phe Ser Leu Thr 530 535 540 Gly Tyr Gly Val Asn
Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 545 550 555 560 Trp Leu
Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 565 570 575
Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val 580
585 590 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr
Tyr 595 600 605 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val
Met Asp Tyr 610 615 620 Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser
Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Asp 645 650 655 Ile Val Leu Thr Gln Ser
Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 660 665 670 Arg Ala Thr Ile
Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 675 680 685 Thr Ser
Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 690 695 700
Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg 705
710 715 720 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile
His Pro 725 730 735 Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln
Gln Ser Arg Lys 740 745 750 Val Pro Trp Thr Phe Gly Gly Gly Thr Lys
Leu Glu Ile Lys Arg 755 760 765 <210> SEQ ID NO 228
<211> LENGTH: 2337 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H7-G281 HL (DNA) <400>
SEQUENCE: 228 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttgtga caaaactcac 840 acatccccac cgtgctcagc
acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg cggtggctcg
ggcggtggtg gatctggagg aggtgggagt 1560 gggaattctc aggtgcagct
gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 1620 ctgtccatca
catgcaccgt ctcagggttc tcattaaccg gctatggtgt aaactgggtt 1680
cgccagcctc caggaaaggg tctggagtgg ctgggaatga tatggggtga tggaagcaca
1740 gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc
caagagccaa 1800 gttttcttaa aaatgaacag tctgcaaact gatgacacag
ccagatacta ctgtgctcga 1860 gatggttata gtaactttca ttactatgtt
atggactact ggggtcaagg aacctcagtc 1920 accgtctcct ctgggggtgg
aggctctggt ggcggtggat ccggcggagg tgggtcgggt 1980 ggcggcggat
ctgacattgt gctcacccaa tctccagctt ctttggctgt gtctctaggt 2040
cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta
2100 atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc
tgctgctagc 2160 aacgtagaat ctggggtccc tgccaggttt agtggcagtg
ggtctgggac agactttagc 2220 ctcaacatcc atcctgtgga ggaggatgat
attgcaatgt atttctgtca gcaaagtagg 2280 aaggttccat ggacgttcgg
tggaggcacc aagctggaaa tcaaacgtta atctaga 2337 <210> SEQ ID NO
229 <211> LENGTH: 772 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7cscIgG1-STD1-2e12HL (w/2E12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(519) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (520)..(640) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (641)..(660) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (661)..(772) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 229 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Cys 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Cys Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Gln Val
Gln Leu Lys Glu Ser Gly Pro 515 520 525 Gly Leu Val Ala Pro Ser Gln
Ser Leu Ser Ile Thr Cys Thr Val Ser 530 535 540 Gly Phe Ser Leu Thr
Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 545 550 555 560 Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 565 570 575
Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 580
585 590 Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp
Asp 595 600 605 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn
Phe His Tyr 610 615 620 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Gly 645 650 655 Gly Gly Gly Ser Asp Ile
Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 660 665 670 Val Ser Leu Gly
Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 675 680 685 Val Glu
Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 690 695 700
Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 705
710 715 720 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp
Phe Ser 725 730 735 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala
Met Tyr Phe Cys 740 745 750 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe
Gly Gly Gly Thr Lys Leu 755 760 765 Glu Ile Lys Arg 770 <210>
SEQ ID NO 230 <211> LENGTH: 45 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: scs(s) -hIgG1 (DNA) <400>
SEQUENCE: 230 gagcccaaat cttctgacaa aactcacaca tctccaccga gctca 45
<210> SEQ ID NO 231 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: scs(s) -hIgG1 (AA)
<400> SEQUENCE: 231 Glu Pro Lys Ser Ser Asp Lys Thr His Thr
Ser Pro Pro Ser Ser 1 5 10 15 <210> SEQ ID NO 232 <211>
LENGTH: 45 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker polynucleotide <220> FEATURE:
<223> OTHER INFORMATION: ccc(s) (DNA) <400> SEQUENCE:
232 gagcccaaat cttgtgacaa aactcacaca tctccaccgt gctca 45
<210> SEQ ID NO 233 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: ccc(s) (AA) <400>
SEQUENCE: 233 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro
Cys Ser 1 5 10 15 <210> SEQ ID NO 234 <211> LENGTH: 24
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H8 (PN) <400> SEQUENCE: 234 gggtctccac
cttctccgaa ttct 24 <210> SEQ ID NO 235 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <400> SEQUENCE: 235 Gly Ser Pro Pro Ser Pro Asn Ser 1
5 <210> SEQ ID NO 236 <211> LENGTH: 21 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H9 (PN) <400> SEQUENCE: 236 tctccacctt ctccgaattc t 21
<210> SEQ ID NO 237 <211> LENGTH: 7 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H9 (AA) <400>
SEQUENCE: 237 Ser Pro Pro Ser Pro Asn Ser 1 5 <210> SEQ ID NO
238 <400> SEQUENCE: 238 000 <210> SEQ ID NO 239
<400> SEQUENCE: 239 000 <210> SEQ ID NO 240 <211>
LENGTH: 51 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker polynucleotide <220> FEATURE:
<223> OTHER INFORMATION: Linker H11 (PN) <400>
SEQUENCE: 240 gagcccacat ctaccgacaa aactcacaca tctccaccca
gcccgaattc t 51 <210> SEQ ID NO 241 <211> LENGTH: 17
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H11 (AA) <400> SEQUENCE: 241 Glu Pro Thr Ser Thr Asp Lys Thr
His Thr Ser Pro Pro Ser Pro Asn 1 5 10 15 Ser <210> SEQ ID NO
242 <211> LENGTH: 51 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H12 (PN)
<400> SEQUENCE: 242 gagcccacat ctaccgacaa aactcacaca
tctccaccca gcccgaattc t 51 <210> SEQ ID NO 243 <211>
LENGTH: 17 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H12 (AA) <400> SEQUENCE: 243 Glu Pro Thr
Ser Thr Asp Lys Thr His Thr Cys Pro Pro Cys Pro Asn 1 5 10 15 Ser
<210> SEQ ID NO 244 <211> LENGTH: 51 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H13 (PN) <400> SEQUENCE: 244 gagcccacat ctaccgacaa
aactcacaca tctccaccca gcccgaattc t 51 <210> SEQ ID NO 245
<211> LENGTH: 17 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H13 (AA) <400>
SEQUENCE: 245 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro
Cys Pro Asn 1 5 10 15 Ser <210> SEQ ID NO 246 <211>
LENGTH: 36 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker polynucleotide <220> FEATURE:
<223> OTHER INFORMATION: Linker H15 (PN) <400>
SEQUENCE: 246 ggcggtggtg gctcctgtcc accttgtccg aattct 36
<210> SEQ ID NO 247 <211> LENGTH: 12 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H15 (AA) <400>
SEQUENCE: 247 Gly Gly Gly Gly Ser Cys Pro Pro Cys Pro Asn Ser 1 5
10 <210> SEQ ID NO 248 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H16 (PN) <400> SEQUENCE: 248 ctgtctgtga aagctgactt
cctcactcca tccatcggga attct 45 <210> SEQ ID NO 249
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H16 (AA) <400>
SEQUENCE: 249 Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly
Asn Ser 1 5 10 15 <210> SEQ ID NO 250 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H17 (PN) <400> SEQUENCE: 250 ctgtctgtga
aagctgactt cctcactcca tccatctcct gtccaccttg cccgaattct 60
<210> SEQ ID NO 251 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H17 (AA) <400>
SEQUENCE: 251 Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Ser
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 252
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H18 (PN) <400>
SEQUENCE: 252 ctgtctgtgc tcgctaactt cagtcagcca gagatcggga attct 45
<210> SEQ ID NO 253 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H18 (AA) <400>
SEQUENCE: 253 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly
Asn Ser 1 5 10 15 <210> SEQ ID NO 254 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H19 (PN) <400> SEQUENCE: 254 ctgtctgtgc
tcgctaactt cagtcagcca gagatctcct gtccaccttg cccgaattct 60
<210> SEQ ID NO 255 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H19 (AA) <400>
SEQUENCE: 255 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Ser
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 256
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H20 (PN) <400>
SEQUENCE: 256 ctgaaaatcc aggagagggt cagtaagcca aagatctcga attct 45
<210> SEQ ID NO 257 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H20 (AA) <400>
SEQUENCE: 257 Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser
Asn Ser 1 5 10 15 <210> SEQ ID NO 258 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polypeptide <220> FEATURE: <223> OTHER
INFORMATION: Linker H21 (PN) <400> SEQUENCE: 258 ctgaaaatcc
aggagagggt cagtaagcca aagatctcct gtccaccttg cccgaattct 60
<210> SEQ ID NO 259 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H21 (AA) <400>
SEQUENCE: 259 Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 260
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polypeptide <220>
FEATURE: <223> OTHER INFORMATION: Linker H22 (PN) <400>
SEQUENCE: 260 ctggatgtga gtgagaggcc ttttcctcca cacatccaga attct 45
<210> SEQ ID NO 261 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H22 (AA) <400>
SEQUENCE: 261 Leu Asp Val Ser Glu Arg Pro Phe Pro Pro His Ile Gln
Asn Ser 1 5 10 15 <210> SEQ ID NO 262 <211> LENGTH: 63
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H23 (PN) <400> SEQUENCE: 262 ctggatgtga
gtgagaggcc ttttcctcca cacatccagt cctgtccacc ttgcccgaat 60 tct 63
<210> SEQ ID NO 263 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H23 (AA) <400>
SEQUENCE: 263 Leu Asp Val Ser Glu Arg Pro Phe Pro Pro His Ile Gln
Ser Cys Pro 1 5 10 15 Pro Cys Pro Asn Ser 20 <210> SEQ ID NO
264 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H24
(DNA) <400> SEQUENCE: 264 cgggaacagc tggcagaggt cactttgagc
ttgaaagcga attct 45 <210> SEQ ID NO 265 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H24 (AA) <400> SEQUENCE: 265 Arg Glu Gln Leu Ala Glu Val Thr
Leu Ser Leu Lys Ala Asn Ser 1 5 10 15 <210> SEQ ID NO 266
<211> LENGTH: 60 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H25 (PN) <400>
SEQUENCE: 266 cgggaacagc tggcagaggt cactttgagc gtgaaagctt
gtccaccctg cccgaattct 60 <210> SEQ ID NO 267 <211>
LENGTH: 20 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H25 (AA) <400> SEQUENCE: 267 Arg Glu Gln
Leu Ala Glu Val Thr Leu Ser Leu Lys Ala Cys Pro Pro 1 5 10 15 Cys
Pro Asn Ser 20 <210> SEQ ID NO 268 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H26 (PN) <400> SEQUENCE: 268 cggattcacc
agatgaactc cgagttgagc gtgctcgcga attct 45 <210> SEQ ID NO 269
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H26 (AA) <400>
SEQUENCE: 269 Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala
Asn Ser 1 5 10 15 <210> SEQ ID NO 270 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H27 (PN) <400> SEQUENCE: 270 cggattcacc
agatgaactc cgagttgagc gtgctcgctt gtccaccctg cccgaattct 60
<210> SEQ ID NO 271 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H27 (AA) <400>
SEQUENCE: 271 Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 272
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H28 (PN) <400>
SEQUENCE: 272 gataccaaag ggaagaacgt cctcgagaag atcttctcga attct 45
<210> SEQ ID NO 273 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H28 (AA) <400>
SEQUENCE: 273 Asp Thr Lys Gly Lys Asn Val Leu Glu Lys Ile Phe Ser
Asn Ser 1 5 10 15 <210> SEQ ID NO 274 <211> LENGTH: 63
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H29 (PN) <400> SEQUENCE: 274 gataccaaag
ggaagaacgt cctcgagaag atcttcgact cctgtccacc ttgcccgaat 60 tct 63
<210> SEQ ID NO 275 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H29 (AA) <400>
SEQUENCE: 275 Asp Thr Lys Gly Lys Asn Val Leu Glu Lys Ile Phe Asp
Ser Cys Pro 1 5 10 15 Pro Cys Pro Asn Ser 20 <210> SEQ ID NO
276 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H30 (PN)
<400> SEQUENCE: 276 ctgccacctg agacacagga gagtcaagaa
gtcaccctga attct 45 <210> SEQ ID NO 277 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H30 (AA) <400> SEQUENCE: 277 Leu Pro Pro Glu Thr Gln Glu Ser
Gln Glu Val Thr Leu Asn Ser 1 5 10 15 <210> SEQ ID NO 278
<211> LENGTH: 63 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H31 (PN) <400>
SEQUENCE: 278 ctgccacctg agacacagga gagtcaagaa gtcaccctgt
cctgtccacc ttgcccgaat 60 tct 63 <210> SEQ ID NO 279
<211> LENGTH: 21 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H31 (AA) <400>
SEQUENCE: 279 Leu Pro Pro Glu Thr Gln Glu Ser Gln Glu Val Thr Leu
Ser Cys Pro 1 5 10 15 Pro Cys Pro Asn Ser 20 <210> SEQ ID NO
280 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H32 (PN)
<400> SEQUENCE: 280 cggattcacc tgaacgtgtc cgagaggccc
tttcctccga attct 45 <210> SEQ ID NO 281 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H32 (AA) <400> SEQUENCE: 281 Arg Ile His Leu Asn Val Ser Glu
Arg Pro Phe Pro Pro Asn Ser 1 5 10 15 <210> SEQ ID NO 282
<211> LENGTH: 60 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H33 (PN) <400>
SEQUENCE: 282 cggattcacc tgaacgtgtc cgagaggccc tttcctccct
gtccaccctg cccgaattct 60 <210> SEQ ID NO 283 <211>
LENGTH: 20 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H33 (AA) <400> SEQUENCE: 283 Arg Ile His
Leu Asn Val Ser Glu Arg Pro Phe Pro Pro Cys Pro Pro 1 5 10 15 Cys
Pro Asn Ser 20 <210> SEQ ID NO 284 <400> SEQUENCE: 284
000 <210> SEQ ID NO 285 <400> SEQUENCE: 285 000
<210> SEQ ID NO 286 <400> SEQUENCE: 286 000 <210>
SEQ ID NO 287 <400> SEQUENCE: 287 000 <210> SEQ ID NO
288 <211> LENGTH: 39 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H36 (PN) <400>
SEQUENCE: 288 gggtgtccac cttgtccagg cggtggtgga tcgaattct 39
<210> SEQ ID NO 289 <211> LENGTH: 13 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H36 (AA) <400>
SEQUENCE: 289 Gly Cys Pro Pro Cys Pro Gly Gly Gly Gly Ser Asn Ser 1
5 10 <210> SEQ ID NO 290 <400> SEQUENCE: 290 000
<210> SEQ ID NO 291 <400> SEQUENCE: 291 000 <210>
SEQ ID NO 292 <400> SEQUENCE: 292 000 <210> SEQ ID NO
293 <400> SEQUENCE: 293 000 <210> SEQ ID NO 294
<400> SEQUENCE: 294 000 <210> SEQ ID NO 295 <400>
SEQUENCE: 295 000 <210> SEQ ID NO 296 <211> LENGTH: 27
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H40 (PN) <400> SEQUENCE: 296 ggatgtccac
cttgtcccgc gaattct 27 <210> SEQ ID NO 297 <211> LENGTH:
9 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H40 (AA) <400> SEQUENCE: 297 Gly Cys Pro Pro Cys Pro Ala Asn
Ser 1 5 <210> SEQ ID NO 298 <400> SEQUENCE: 298 000
<210> SEQ ID NO 299 <211> LENGTH: 9 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: Linker H41 <400> SEQUENCE: 299 Gly Cys Pro Pro
Cys Pro Ala Asn Ser 1 5 <210> SEQ ID NO 300 <400>
SEQUENCE: 300 000 <210> SEQ ID NO 301 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H42(AA) <400> SEQUENCE: 301 Gly Cys Pro Pro Cys Pro Asn Ser 1
5 <210> SEQ ID NO 302 <400> SEQUENCE: 302 000
<210> SEQ ID NO 303 <400> SEQUENCE: 303 000 <210>
SEQ ID NO 304 <211> LENGTH: 36 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H44 (PN) <400> SEQUENCE: 304 ggaggagcta gttgtccacc
ttgtcccggg aattct 36 <210> SEQ ID NO 305 <211> LENGTH:
12 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H44 (AA) <400> SEQUENCE: 305 Gly Gly Ala Ser Cys Pro Pro Cys
Pro Gly Asn Ser 1 5 10 <210> SEQ ID NO 306 <211>
LENGTH: 36 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker polynucleotide <220> FEATURE:
<223> OTHER INFORMATION: Linker H45 (PN) <400>
SEQUENCE: 306 ggaggagcca gttgtccacc ttgtgccggg aattct 36
<210> SEQ ID NO 307 <211> LENGTH: 12 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H45 (AA) <400>
SEQUENCE: 307 Gly Gly Ala Ser Cys Pro Pro Cys Ala Gly Asn Ser 1 5
10 <210> SEQ ID NO 308 <211> LENGTH: 33 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H46 <400> SEQUENCE: 308 ggaggagcca gttgtccacc
ttgtgcgaat tct 33 <210> SEQ ID NO 309 <211> LENGTH: 11
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H46 <400> SEQUENCE: 309 Gly Gly Ala Ser Cys Pro Pro Cys Ala
Asn Ser 1 5 10 <210> SEQ ID NO 310 <211> LENGTH: 15
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H47 <400> SEQUENCE: 310 Leu Ser Val Lys Ala Asp Phe Leu Thr
Pro Ser Ile Gly Asn Ser 1 5 10 15 <210> SEQ ID NO 311
<211> LENGTH: 12 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H48 <400> SEQUENCE: 311
Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly Asn Ser 1 5 10 <210>
SEQ ID NO 312 <400> SEQUENCE: 312 000 <210> SEQ ID NO
313 <211> LENGTH: 15 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H50 <400> SEQUENCE: 313
Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly Asn Ser 1 5 10
15 <210> SEQ ID NO 314 <211> LENGTH: 15 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H51
<400> SEQUENCE: 314 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro
Glu Ile Gly Asn Ser 1 5 10 15 <210> SEQ ID NO 315 <211>
LENGTH: 11 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic peptide <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <400> SEQUENCE: 315 Ser
Gln Pro Glu Ile Val Pro Ile Ser Asn Ser 1 5 10 <210> SEQ ID
NO 316 <211> LENGTH: 16 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H53 <400> SEQUENCE: 316
Ser Gln Pro Glu Ile Val Pro Ile Ser Cys Pro Pro Cys Pro Asn Ser 1 5
10 15 <210> SEQ ID NO 317 <211> LENGTH: 19 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H54
<400> SEQUENCE: 317 Ser Val Leu Ala Asn Phe Ser Gln Pro Glu
Ile Ser Cys Pro Pro Cys 1 5 10 15 Pro Asn Ser <210> SEQ ID NO
318 <211> LENGTH: 15 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H55 <400> SEQUENCE: 318
Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn Ser 1 5 10
15 <210> SEQ ID NO 319 <211> LENGTH: 12 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H56
<400> SEQUENCE: 319 Gln Met Asn Ser Glu Leu Ser Val Leu Ala
Asn Ser 1 5 10 <210> SEQ ID NO 320 <211> LENGTH: 10
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H57 <400> SEQUENCE: 320 Val Ser Glu Arg Pro Phe Pro Pro Asn
Ser 1 5 10 <210> SEQ ID NO 321 <211> LENGTH: 15
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H58 <400> SEQUENCE: 321 Lys Pro Phe Phe Thr Cys Gly Ser Ala
Asp Thr Cys Pro Asn Ser 1 5 10 15 <210> SEQ ID NO 322
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H59 <400> SEQUENCE: 322
Lys Pro Phe Phe Thr Cys Gly Ser Ala Asp Thr Cys Pro Asn Ser 1 5 10
15 <210> SEQ ID NO 323 <211> LENGTH: 14 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H60
<400> SEQUENCE: 323 Gln Tyr Asn Cys Pro Gly Gln Tyr Thr Phe
Ser Met Asn Ser 1 5 10 <210> SEQ ID NO 324 <211>
LENGTH: 21 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H61 <400> SEQUENCE: 324 Glu Pro Ala Phe
Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5 10 15 Asp Cys
Pro Asn Ser 20 <210> SEQ ID NO 325 <211> LENGTH: 21
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H62 <400> SEQUENCE: 325 Gln Arg His Asn Asn Ser Ser Leu Asn
Thr Arg Thr Gln Lys Ala Arg 1 5 10 15 His Cys Pro Asn Ser 20
<210> SEQ ID NO 326 <211> LENGTH: 19 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H63 <400>
SEQUENCE: 326 Asn Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr
Glu Ser Tyr 1 5 10 15 Cys Asn Ser <210> SEQ ID NO 327
<211> LENGTH: 21 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <400> SEQUENCE: 327 Gln
Arg His Asn Asn Ser Ser Leu Asn Thr Arg Thr Gln Lys Ala Arg 1 5 10
15 His Cys Pro Asn Ser 20 <210> SEQ ID NO 328 <211>
LENGTH: 21 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H65 <400> SEQUENCE: 328 Glu Pro Ala Phe
Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5 10 15 Asp Cys
Pro Asn Ser 20 <210> SEQ ID NO 329 <211> LENGTH: 21
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H66 <400> SEQUENCE: 329 Glu Pro Ala Phe Thr Pro Gly Pro Asn
Ile Glu Leu Gln Lys Asp Ser 1 5 10 15 Asp Cys Pro Asn Ser 20
<210> SEQ ID NO 330 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H67 <400>
SEQUENCE: 330 Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln
Lys Asp Ser 1 5 10 15 Asp Cys Pro Asn Ser 20 <210> SEQ ID NO
331 <211> LENGTH: 118 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H68 <400> SEQUENCE: 331
Arg Thr Arg Tyr Leu Gln Val Ser Gln Gln Leu Gln Gln Thr Asn Arg 1 5
10 15 Val Leu Glu Val Thr Asn Ser Ser Leu Arg Gln Gln Leu Arg Leu
Lys 20 25 30 Ile Thr Gln Leu Gly Gln Ser Ala Glu Asp Leu Gln Gly
Ser Arg Arg 35 40 45 Glu Leu Ala Gln Ser Gln Glu Ala Leu Gln Val
Glu Gln Arg Ala His 50 55 60 Gln Ala Ala Glu Gly Gln Leu Gln Ala
Cys Gln Ala Asp Arg Gln Lys 65 70 75 80 Thr Lys Glu Thr Leu Gln Ser
Glu Glu Gln Gln Arg Arg Ala Leu Glu 85 90 95 Gln Lys Leu Ser Asn
Met Glu Asn Arg Leu Lys Pro Phe Phe Thr Cys 100 105 110 Gly Ser Ala
Asp Thr Cys 115 <210> SEQ ID NO 332 <211> LENGTH: 10
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD-20 VL
CDR1 <400> SEQUENCE: 332 Arg Ala Ser Ser Ser Val Ser Tyr Ile
His 1 5 10 <210> SEQ ID NO 333 <211> LENGTH: 10
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD-20 VL
CDR1 <400> SEQUENCE: 333 Arg Ala Ser Ser Ser Val Ser Tyr Ile
Val 1 5 10 <210> SEQ ID NO 334 <211> LENGTH: 9
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD-20 VL
CDR3 <400> SEQUENCE: 334 Gln Gln Trp Ser Phe Asn Pro Pro Thr
1 5 <210> SEQ ID NO 335 <211> LENGTH: 9 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: anti-CD-20 VL CDR3
<400> SEQUENCE: 335 Gln Gln Tyr Ser Phe Asn Pro Pro Thr 1 5
<210> SEQ ID NO 336 <211> LENGTH: 17 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD-20 VH CDR2 <400>
SEQUENCE: 336 Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln
Lys Phe Lys 1 5 10 15 Gly <210> SEQ ID NO 337 <211>
LENGTH: 17 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
anti-CD-20 VH CDR2 <400> SEQUENCE: 337 Ala Ile Tyr Pro Gly
Asn Gly Glu Thr Ser Tyr Asn Gln Lys Phe Lys 1 5 10 15 Gly
<210> SEQ ID NO 338 <211> LENGTH: 12 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD20 VH CDR3 <400>
SEQUENCE: 338 Ser Val Tyr Tyr Ser Asn Tyr Trp Tyr Phe Asp Leu 1 5
10 <210> SEQ ID NO 339 <211> LENGTH: 12 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: anti-CD20 VH CDR3
<400> SEQUENCE: 339 Ser Val Tyr Tyr Gly Gly Tyr Trp Tyr Phe
Asp Leu 1 5 10 <210> SEQ ID NO 340 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 340 Ser Tyr Tyr Ser Asn Ser Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 341 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 341 Ser Tyr Tyr Ser Gly Gly Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 342 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 342 Ser Tyr Lys Ser Asn Ser Tyr Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 343 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 343 Ser Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 344 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD-20 VH
CDR3 <400> SEQUENCE: 344 Ser Tyr Lys Ser Asn Ser Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 345 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 345 Ser Tyr Lys Ser Gly Gly Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 346 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
core (2H7) sequence <400> SEQUENCE: 346 Gly Cys Pro Pro Cys
Pro Asn Ser 1 5 <210> SEQ ID NO 347 <211> LENGTH: 4
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Extension
sequence <400> SEQUENCE: 347 Ala Pro Glu Leu 1 <210>
SEQ ID NO 348 <211> LENGTH: 9 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide <220>
FEATURE: <223> OTHER INFORMATION: Extension sequence
<400> SEQUENCE: 348 Ala Pro Glu Leu Gly Gly Gly Gly Ser 1 5
<210> SEQ ID NO 349 <211> LENGTH: 14 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Extension sequence <400>
SEQUENCE: 349 Ala Pro Glu Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser 1 5 10 <210> SEQ ID NO 350 <211> LENGTH: 19
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Extension
sequence <400> SEQUENCE: 350 Ala Pro Glu Leu Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly 1 5 10 15 Gly Gly Ser <210>
SEQ ID NO 351 <211> LENGTH: 12 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Extended scorpion linker <400>
SEQUENCE: 351 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu 1 5
10 <210> SEQ ID NO 352 <211> LENGTH: 17 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Extended scorpion linker
<400> SEQUENCE: 352 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro
Glu Leu Gly Gly Gly Gly 1 5 10 15 Ser <210> SEQ ID NO 353
<211> LENGTH: 22 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Extended scorpion linker <400> SEQUENCE: 353 Gly
Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu Gly Gly Gly Gly 1 5 10
15 Ser Gly Gly Gly Gly Ser 20 <210> SEQ ID NO 354 <211>
LENGTH: 27 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Extended scorpion linker <400> SEQUENCE: 354 Gly Cys Pro Pro
Cys Pro Asn Ser Ala Pro Glu Leu Gly Gly Gly Gly 1 5 10 15 Ser Gly
Gly Gly Gly Ser Gly Gly Gly Gly Ser 20 25 <210> SEQ ID NO 355
<400> SEQUENCE: 355 000 <210> SEQ ID NO 356 <400>
SEQUENCE: 356 000 <210> SEQ ID NO 357 <400> SEQUENCE:
357 000 <210> SEQ ID NO 358 <400> SEQUENCE: 358 000
<210> SEQ ID NO 359 <400> SEQUENCE: 359 000 <210>
SEQ ID NO 360 <400> SEQUENCE: 360 000 <210> SEQ ID NO
361 <400> SEQUENCE: 361 000 <210> SEQ ID NO 362
<400> SEQUENCE: 362 000 <210> SEQ ID NO 363 <400>
SEQUENCE: 363 000 <210> SEQ ID NO 364 <400> SEQUENCE:
364 000 <210> SEQ ID NO 365 <400> SEQUENCE: 365 000
<210> SEQ ID NO 366 <211> LENGTH: 10 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: partial CH3 sequence <400>
SEQUENCE: 366 Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 1 5 10
<210> SEQ ID NO 367 <211> LENGTH: 9 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Partial CH3 sequence <400>
SEQUENCE: 367 Gln Lys Ser Leu Ser Leu Ser Pro Gly 1 5 <210>
SEQ ID NO 368 <211> LENGTH: 1 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Partial CH3 sequence <400>
SEQUENCE: 368 Gln 1 <210> SEQ ID NO 369 <211> LENGTH: 3
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Partial CH3
sequence <400> SEQUENCE: 369 Gln Lys Ser 1 <210> SEQ ID
NO 370 <211> LENGTH: 6 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Partial CH3 sequence <400> SEQUENCE: 370
Gln Lys Ser Leu Ser Leu 1 5 <210> SEQ ID NO 371 <211>
LENGTH: 5 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Partial CH3 sequence <400> SEQUENCE: 371 Gln Lys Ser Leu Ser
1 5 <210> SEQ ID NO 372 <211> LENGTH: 5 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion linker <400>
SEQUENCE: 372 Gly Cys Pro Pro Cys 1 5 <210> SEQ ID NO 373
<211> LENGTH: 12 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker <400> SEQUENCE: 373 Gln Tyr Asn
Cys Pro Gly Gln Tyr Thr Phe Ser Met 1 5 10 <210> SEQ ID NO
374 <211> LENGTH: 11 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion linker <400> SEQUENCE: 374 Pro
Phe Phe Thr Cys Gly Ser Ala Asp Thr Cys 1 5 10 <210> SEQ ID
NO 375 <211> LENGTH: 18 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion linker <400> SEQUENCE: 375 Glu
Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5 10
15 Asp Cys <210> SEQ ID NO 376 <211> LENGTH: 18
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
<400> SEQUENCE: 376 Gln Arg His Asn Asn Ser Ser Leu Asn Thr
Arg Thr Gln Lys Ala Arg 1 5 10 15 His Cys <210> SEQ ID NO 377
<211> LENGTH: 17 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker <400> SEQUENCE: 377 Asn Ser Leu
Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr 1 5 10 15 Cys
<210> SEQ ID NO 378 <211> LENGTH: 10 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD20 VL CDR1 (TRU-015)
<400> SEQUENCE: 378 Arg Ala Ser Ser Ser Val Ser Tyr Met His 1
5 10 <210> SEQ ID NO 379 <211> LENGTH: 13 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: anti-CD20 VH CDR3 (TRU-015)
<400> SEQUENCE: 379 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val 1 5 10
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 379
<210> SEQ ID NO 1 <211> LENGTH: 795 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polynucleotide - anti-CD20
(2H7) LH (DNA) <400> SEQUENCE: 1 atggattttc aagtgcagat
tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60 agaggacaaa
ttgttctctc ccagtctcca gcaatcctgt ctgcatctcc aggggagaag 120
gtcacaatga cttgcagggc cagctcaagt gtaagttaca tgcactggta ccagcagaag
180 ccaggatcct cccccaaacc ctggatttat gccccatcca acctggcttc
tggagtccct 240 gctcgcttca gtggcagtgg gtctgggacc tcttactctc
tcacaatcag cagagtggag 300 gctgaagatg ctgccactta ttactgccag
cagtggagtt ttaacccacc cacgttcggt 360 gctgggacca agctggagct
gaaagatggc ggtggctcgg gcggtggtgg atctggagga 420 ggtgggagct
ctcaggctta tctacagcag tctggggctg agtcggtgag gcctggggcc 480
tcagtgaaga tgtcctgcaa ggcttctggc tacacattta ccagttacaa tatgcactgg
540 gtaaagcaga cacctagaca gggcctggaa tggattggag ctatttatcc
aggaaatggt 600 gatacttcct acaatcagaa gttcaagggc aaggccacac
tgactgtaga caaatcctcc 660 agcacagcct acatgcagct cagcagcctg
acatctgaag actctgcggt ctatttctgt 720 gcaagagtgg tgtactatag
taactcttac tggtacttcg atgtctgggg cacagggacc 780 acggtcaccg tctct
795 <210> SEQ ID NO 2 <211> LENGTH: 265 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic polypeptide -
anti-CD20 (2H7) LH (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <400> SEQUENCE: 2 Met Asp Phe Gln Val Gln Ile
Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg
Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala
Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser
Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55
60 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro
65 70 75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu
Thr Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr
Cys Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly
Thr Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln
Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys
Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn
Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185
190 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe
195 200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr
Ala Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala
Val Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser
Tyr Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr
Val Ser 260 265 <210> SEQ ID NO 3 <211> LENGTH: 813
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - anti-CD28 (2e12) LH (DNA) <400> SEQUENCE: 3
atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc
60 agaggagtcg acattgtgct cacccaatct ccagcttctt tggctgtgtc
tctaggtcag 120 agagccacca tctcctgcag agccagtgaa agtgttgaat
attatgtcac aagtttaatg 180 cagtggtacc aacagaaacc aggacagcca
cccaaactcc tcatctctgc tgctagcaac 240 gtagaatctg gggtccctgc
caggtttagt ggcagtgggt ctgggacaga ctttagcctc 300 aacatccatc
ctgtggagga ggatgatatt gcaatgtatt tctgtcagca aagtaggaag 360
gttccatgga cgttcggtgg aggcaccaag ctggaaatca aacggggtgg cggtggatcc
420 ggcggaggtg ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc
aggacctggc 480 ctggtggcgc cctcacagag cctgtccatc acatgcaccg
tctcagggtt ctcattaacc 540 ggctatggtg taaactgggt tcgccagcct
ccaggaaagg gtctggagtg gctgggaatg 600 atatggggtg atggaagcac
agactataat tcagctctca aatccagact atcgatcacc 660 aaggacaact
ccaagagcca agttttctta aaaatgaaca gtctgcaaac tgatgacaca 720
gccagatact actgtgcccg agatggttat agtaactttc attactatgt tatggactac
780 tggggtcaag gaacctcagt caccgtctcc tct 813 <210> SEQ ID NO
4 <211> LENGTH: 271 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic polypeptide - anti-CD28 (2e12) LH (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(23) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (24)..(135) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(136)..(150) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(151)..(271) <223> OTHER INFORMATION: VH <400>
SEQUENCE: 4 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser
Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Val Asp Ile Val Leu Thr
Gln Ser Pro Ala 20 25 30 Ser Leu Ala Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala 35 40 45 Ser Glu Ser Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln 50 55 60 Gln Lys Pro Gly Gln Pro
Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 65 70 75 80 Val Glu Ser Gly
Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 85 90 95 Asp Phe
Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met 100 105 110
Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly 115
120 125 Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly
Gly 130 135 140 Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser
Gly Pro Gly 145 150 155 160 Leu Val Ala Pro Ser Gln Ser Leu Ser Ile
Thr Cys Thr Val Ser Gly 165 170 175 Phe Ser Leu Thr Gly Tyr Gly Val
Asn Trp Val Arg Gln Pro Pro Gly 180 185 190 Lys Gly Leu Glu Trp Leu
Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 195 200 205 Tyr Asn Ser Ala
Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 210 215 220 Lys Ser
Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 225 230 235
240 Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr
245 250 255 Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser
Ser 260 265 270 <210> SEQ ID NO 5 <211> LENGTH: 828
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - anti-CD28 (2e12) HL
(DNA) <400> SEQUENCE: 5 atggattttc aagtgcagat tttcagcttc
ctgctaatca gtgcttcagt cataatgtcc 60 agaggagtcc aggtgcagct
gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 120 ctgtccatca
catgcaccgt ctcagggttc tcattaaccg gctatggtgt aaactgggtt 180
cgccagcctc caggaaaggg tctggagtgg ctgggaatga tatggggtga tggaagcaca
240 gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc
caagagccaa 300 gttttcttaa aaatgaacag tctgcaaact gatgacacag
ccagatacta ctgtgcccga 360 gatggttata gtaactttca ttactatgtt
atggactact ggggtcaagg aacctcagtc 420 accgtctcct ctgggggtgg
aggctctggt ggcggtggat ccggcggagg tgggtcgggt 480 ggcggcggat
ctgacattgt gctcacccaa tctccagctt ctttggctgt gtctctaggt 540
cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta
600 atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc
tgctgctagc 660 aacgtagaat ctggggtccc tgccaggttt agtggcagtg
ggtctgggac agactttagc 720 ctcaacatcc atcctgtgga ggaggatgat
attgcaatgt atttctgtca gcaaagtagg 780 aaggttccat ggacgttcgg
tggaggcacc aagctggaaa tcaaacgt 828 <210> SEQ ID NO 6
<211> LENGTH: 276 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic polypeptide - anti-CD28 (2e12) HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(23) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (24)..(144) <223> OTHER INFORMATION: VH <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(164) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(165)..(276) <223> OTHER INFORMATION: VL <400>
SEQUENCE: 6 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser
Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Val Gln Val Gln Leu Lys
Glu Ser Gly Pro 20 25 30 Gly Leu Val Ala Pro Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser 35 40 45 Gly Phe Ser Leu Thr Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro 50 55 60 Gly Lys Gly Leu Glu Trp
Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 65 70 75 80 Asp Tyr Asn Ser
Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 85 90 95 Ser Lys
Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 100 105 110
Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 115
120 125 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser
Ser 130 135 140 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly 145 150 155 160 Gly Gly Gly Ser Asp Ile Val Leu Thr Gln
Ser Pro Ala Ser Leu Ala 165 170 175 Val Ser Leu Gly Gln Arg Ala Thr
Ile Ser Cys Arg Ala Ser Glu Ser 180 185 190 Val Glu Tyr Tyr Val Thr
Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 195 200 205 Gly Gln Pro Pro
Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 210 215 220 Gly Val
Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 225 230 235
240 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys
245 250 255 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr
Lys Leu 260 265 270 Glu Ile Lys Arg 275 <210> SEQ ID NO 7
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - Heavy Chain GSP1 <400>
SEQUENCE: 7 aggtgctgga ggggacagtc actgagctgc 30 <210> SEQ ID
NO 8 <211> LENGTH: 28 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - Nested Heavy Chain
<400> SEQUENCE: 8 gtcacwgtca ctgrctcagg gaartagc 28
<210> SEQ ID NO 9 <211> LENGTH: 33 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - Light Chain GSP1
<400> SEQUENCE: 9 gggtgctgct catgctgtag gtgctgtctt tgc 33
<210> SEQ ID NO 10 <211> LENGTH: 32 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - Nested Light
Chain <400> SEQUENCE: 10 caagaagcac acgactgagg cacctccaga tg
32 <210> SEQ ID NO 11 <211> LENGTH: 36 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - Race
Abridged Anchor Primer <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (24)..(25) <223> OTHER
INFORMATION: n is a, c, g, or t <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (29)..(30) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (34)..(35)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 11 ggccacgcgt cgactagtac gggnngggnn gggnng 36 <210>
SEQ ID NO 12 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - T7 primer
<400> SEQUENCE: 12 gtaatacgac tcactatagg 20 <210> SEQ
ID NO 13 <211> LENGTH: 18 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - M13 reverse primer
<400> SEQUENCE: 13 caggaaacag ctatgacc 18 <210> SEQ ID
NO 14 <211> LENGTH: 15 <212> TYPE: PRT <213>
ORGANISM: Homo sapiens <220> FEATURE: <223> OTHER
INFORMATION: human IgG1 hinge domain <400> SEQUENCE: 14 Glu
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 15 <211> LENGTH: 46 <212> TYPE:
DNA <213> ORGANISM: Homo sapiens <220> FEATURE:
<223> OTHER INFORMATION: hVK3L-F3H3 <400> SEQUENCE: 15
gcgataaagc ttgccgccat ggaagcacca gcgcagcttc tcttcc 46 <210>
SEQ ID NO 16 <211> LENGTH: 50 <212> TYPE: DNA
<213> ORGANISM: Homo sapiens <220> FEATURE: <223>
OTHER INFORMATION: hVK3LF2 <400> SEQUENCE: 16 accagcgcag
cttctcttcc tcctgctact ctggctccca gataccaccg 50 <210> SEQ ID
NO 17 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Homo sapiens
<220> FEATURE: <223> OTHER INFORMATION: hVK3LF12HVL
<400> SEQUENCE: 17 ggctcccaga taccaccggt caaattgttc
tctcccagtc tccag 45 <210> SEQ ID NO 18 <211> LENGTH: 35
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - 2H7VHNheF <400> SEQUENCE: 18 gcgatagcta gccaggctta
tctacagcag tctgg 35 <210> SEQ ID NO 19 <211> LENGTH: 43
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G4SNheR <400> SEQUENCE: 19 gcgatagcta gccccacctc
ctccagatcc accaccgccc gag 43 <210> SEQ ID NO 20 <211>
LENGTH: 35 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 015VHXhoR <400> SEQUENCE: 20 gcgtactcga
ggagacggtg accgtggtcc ctgtg 35 <210> SEQ ID NO 21 <211>
LENGTH: 37 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - G1HC-Xho <400> SEQUENCE: 21 gcagtctcga
gcgagcccaa atcttgtgac aaaactc 37 <210> SEQ ID NO 22
<211> LENGTH: 37 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G1H-S-Xho <400> SEQUENCE: 22
gcagtctcga gcgagcccaa atcttctgac aaaactc 37 <210> SEQ ID NO
23 <211> LENGTH: 36 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - CH3REcoR1 <400>
SEQUENCE: 23 gcgtgagaat tcttacccgg agacagggag aggctc 36 <210>
SEQ ID NO 24 <211> LENGTH: 32 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G1 XBZ-R
<400> SEQUENCE: 24 gcgacgtcta gagtcattta cccggagaca gg 32
<210> SEQ ID NO 25 <211> LENGTH: 54 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G4SLinkR1-S
<400> SEQUENCE: 25 aattatggtg gcggtggctc gggcggtggt
ggatctggag gaggtgggag tggg 54 <210> SEQ ID NO 26 <211>
LENGTH: 54 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - G4SLinkR1-AS <400> SEQUENCE: 26 aattcccact
cccacctcct ccagatccac caccgcccga gccaccgcca ccat 54 <210> SEQ
ID NO 27 <211> LENGTH: 36 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 2E12VLXbaR <400>
SEQUENCE: 27 gcgtgtctag attaacgttt gatttccagc ttggtg 36 <210>
SEQ ID NO 28 <211> LENGTH: 35 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VLR1F
<400> SEQUENCE: 28 gcgatgaatt ctgacattgt gctcacccaa tctcc 35
<210> SEQ ID NO 29 <211> LENGTH: 34 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VHR1F
<400> SEQUENCE: 29 gcgatgaatt ctcaggtgca gctgaaggag tcag 34
<210> SEQ ID NO 30 <211> LENGTH: 37 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 2E12VHXbaR
<400> SEQUENCE: 30 gcgagtctag attaagagga gacggtgact gaggttc
37 <210> SEQ ID NO 31 <211> LENGTH: 27 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
2e12VHdXbaF1 <400> SEQUENCE: 31 gggtctggag tggctgggaa tgatatg
27 <210> SEQ ID NO 32 <211> LENGTH: 28 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
2e12VHdXbaR1 <400> SEQUENCE: 32 attcccagcc actccagacc
ctttcctg 28 <210> SEQ ID NO 33 <211> LENGTH: 23
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - IgBsrG1F <400> SEQUENCE: 33 gagaaccaca ggtgtacacc
ctg 23 <210> SEQ ID NO 34 <211> LENGTH: 25 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - IgBsrG1R
<400> SEQUENCE: 34 gcagggtgta cacctgtggt tctcg 25 <210>
SEQ ID NO 35 <211> LENGTH: 17 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - M13R <400>
SEQUENCE: 35 caggaaacag ctatgac 17 <210> SEQ ID NO 36
<211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - M13F <400> SEQUENCE: 36
gtaaaacgac ggccagtg 18 <210> SEQ ID NO 37 <211> LENGTH:
20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - T7 <400> SEQUENCE: 37 gtaatacgac tcactatagg 20
<210> SEQ ID NO 38
<211> LENGTH: 17 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - pD18F-17 <400> SEQUENCE: 38
aactagagaa cccactg 17 <210> SEQ ID NO 39 <211> LENGTH:
20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - pD18F-20 <400> SEQUENCE: 39 gctaactaga gaacccactg 20
<210> SEQ ID NO 40 <211> LENGTH: 18 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - pD18F-1
<400> SEQUENCE: 40 atacgactca ctataggg 18 <210> SEQ ID
NO 41 <211> LENGTH: 19 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - pD18R-s <400> SEQUENCE:
41 gctctagcat ttaggtgac 19 <210> SEQ ID NO 42 <211>
LENGTH: 18 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - CH3seqF1 <400> SEQUENCE: 42 catgaggctc
tgcacaac 18 <210> SEQ ID NO 43 <211> LENGTH: 18
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - CH3seqF2 <400> SEQUENCE: 43 cctctacagc aagctcac 18
<210> SEQ ID NO 44 <211> LENGTH: 19 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - CH3seqR1
<400> SEQUENCE: 44 ggttcttggt cagctcatc 19 <210> SEQ ID
NO 45 <211> LENGTH: 18 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - CH3seqR2 <400>
SEQUENCE: 45 gtgagcttgc tgtagagg 18 <210> SEQ ID NO 46
<211> LENGTH: 51 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - L1-11R <400> SEQUENCE: 46
gcgatagaat tcccagatcc accaccgccc gagccaccgc caccataatt c 51
<210> SEQ ID NO 47 <211> LENGTH: 36 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - L1-6R <400>
SEQUENCE: 47 gcgatagaat tcccagagcc accgccacca taattc 36 <210>
SEQ ID NO 48 <211> LENGTH: 45 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - L3R <400>
SEQUENCE: 48 gcgtatgaat tccccgagcc accgccaccc ttacccggag acagg 45
<210> SEQ ID NO 49 <211> LENGTH: 49 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - L4R <400>
SEQUENCE: 49 gcgtatgaat tcccagatcc accaccgccc gagccaccgc cacccttac
49 <210> SEQ ID NO 50 <211> LENGTH: 44 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - L5R
<400> SEQUENCE: 50 gcgtatgaat tcccgctgcc tcctccccca
gatccaccac cgcc 44 <210> SEQ ID NO 51 <211> LENGTH: 23
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - IgBsrG1F <400> SEQUENCE: 51 gagaaccaca ggtgtacacc
ctg 23 <210> SEQ ID NO 52 <211> LENGTH: 50 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer - L-CPPCPR
<400> SEQUENCE: 52 gcgatagaat tcggacaagg tggacacccc
ttacccggag acagggagag 50 <210> SEQ ID NO 53 <211>
LENGTH: 41 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - G281LH-NheR <400> SEQUENCE: 53 actgctgcag
ctggaccgcg ctagctccgc cgccacccga c 41 <210> SEQ ID NO 54
<211> LENGTH: 40 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281LH-NheF <400> SEQUENCE:
54 ggcggagcta gcgcggtcca gctgcagcag tctggacctg 40 <210> SEQ
ID NO 55 <211> LENGTH: 37 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - G281-LH-LPinF <400>
SEQUENCE: 55 gcgatcaccg gtgacatcca gatgactcag tctccag 37
<210> SEQ ID NO 56 <211> LENGTH: 37 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-LH-HXhoR
<400> SEQUENCE: 56 gcgatactcg aggagacggt gactgaggtt ccttgac
37 <210> SEQ ID NO 57 <211> LENGTH: 38 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
G281-LH-LEcoF <400> SEQUENCE: 57 gcgatcgaat tcagacatcc
agatgactca gtctccag 38 <210> SEQ ID NO 58 <211> LENGTH:
43 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G281-LH-HXbaR <400> SEQUENCE: 58 gcgattctag
attaggaaga gacggtgact gaggttcctt gac 43
<210> SEQ ID NO 59 <211> LENGTH: 37 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-HL-HF
<400> SEQUENCE: 59 gcgataaccg gtgcggtcca gctgcagcag tctggac
37 <210> SEQ ID NO 60 <211> LENGTH: 52 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
G281-HL-HR3 <400> SEQUENCE: 60 gacccaccac cgcccgagcc
accgccacca gaagagacgg tgactgaggt tc 52 <210> SEQ ID NO 61
<211> LENGTH: 49 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281-HL-HR2 <400> SEQUENCE:
61 actcccgcct cctcctgatc cgccgccacc cgacccacca ccgcccgag 49
<210> SEQ ID NO 62 <211> LENGTH: 42 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-HL-HNheR
<400> SEQUENCE: 62 gagtcatctg gatgtcgcta gcactcccgc
ctcctcctga tc 42 <210> SEQ ID NO 63 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - G281-HL-LNheF <400> SEQUENCE: 63 atcaggagga
ggcgggagtg ctagcgacat ccagatgact cagtc 45 <210> SEQ ID NO 64
<211> LENGTH: 37 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - G281-HL-LXhoR <400> SEQUENCE:
64 gcgatactcg agcctttgat ctccagttcg gtgcctc 37 <210> SEQ ID
NO 65 <211> LENGTH: 42 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - G281-HL-LXhoR <400>
SEQUENCE: 65 gcgatatcta gactcaacct ttgatctcca gttcggtgcc tc 42
<210> SEQ ID NO 66 <211> LENGTH: 37 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - G281-HL-EcoF
<400> SEQUENCE: 66 gcgatagaat tcgcggtcca gctgcagcag tctggac
37 <210> SEQ ID NO 67 <211> LENGTH: 40 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
194-LH-LF1 <400> SEQUENCE: 67 gcgtatgaac cggtgacatc
cagatgacac agactacatc 40 <210> SEQ ID NO 68 <211>
LENGTH: 50 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LF2 <400> SEQUENCE: 68 atccagatga
cacagactac atcctccctg tctgcctctc tgggagacag 50 <210> SEQ ID
NO 69 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LF3 <400> SEQUENCE:
69 gtctgcctct ctgggagaca gagtcaccat cagttgcagg gcaagtcagg ac 52
<210> SEQ ID NO 70 <211> LENGTH: 50 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LF4
<400> SEQUENCE: 70 gttgcagggc aagtcaggac attcgcaatt
atttaaactg gtatcagcag 50 <210> SEQ ID NO 71 <211>
LENGTH: 50 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LF5 <400> SEQUENCE: 71 atttaaactg
gtatcagcag aaaccagatg gaactgttaa actcctgatc 50 <210> SEQ ID
NO 72 <211> LENGTH: 50 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LF6 <400> SEQUENCE:
72 gaactgttaa actcctgatc tactacacat caagattaca ctcaggagtc 50
<210> SEQ ID NO 73 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LF7
<400> SEQUENCE: 73 caagattaca ctcaggagtc ccatcaaggt
tcagtggcag tgggtctgga ac 52 <210> SEQ ID NO 74 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LR7 <400> SEQUENCE: 74 caggttggca
atggtgagag aataatctgt tccagaccca ctgccactga ac 52 <210> SEQ
ID NO 75 <211> LENGTH: 50 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LR6 <400> SEQUENCE:
75 gcaaaagtaa gtggcaatat cttctggttg caggttggca atggtgagag 50
<210> SEQ ID NO 76 <211> LENGTH: 51 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LR5
<400> SEQUENCE: 76 gaacgtccac ggaagcgtat taccctgttg
gcaaaagtaa gtggcaatat c 51 <210> SEQ ID NO 77 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LR4 <400> SEQUENCE: 77 cgtttggtta
ccagtttggt gcctccaccg aacgtccacg gaagcgtatt ac 52 <210> SEQ
ID NO 78 <211> LENGTH: 46 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-LR3 <400> SEQUENCE:
78 accaccgccc gagccaccgc caccccgttt ggttaccagt ttggtg 46
<210> SEQ ID NO 79 <211> LENGTH: 50 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LR2
<400> SEQUENCE: 79 gctagcgctc ccacctcctc cagatccacc
accgcccgag ccaccgccac 50
<210> SEQ ID NO 80 <211> LENGTH: 42 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LH-LR1
<400> SEQUENCE: 80 gttgcagctg gacctcgcta gcgctcccac
ctcctccaga tc 42 <210> SEQ ID NO 81 <211> LENGTH: 54
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer - 194-LH-HF1 <400> SEQUENCE: 81 gatctggagg aggtgggagc
gctagcgagg tccagctgca acagtctgga cctg 54 <210> SEQ ID NO 82
<211> LENGTH: 50 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - 194-HF2 <400> SEQUENCE: 82
agctgcaaca gtctggacct gaactggtga agcctggagc ttcaatgaag 50
<210> SEQ ID NO 83 <211> LENGTH: 50 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HF3
<400> SEQUENCE: 83 agcctggagc ttcaatgaag atttcctgca
aggcctctgg ttactcattc 50 <210> SEQ ID NO 84 <211>
LENGTH: 53 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HF4 <400> SEQUENCE: 84 gcaaggcctc
tggttactca ttcactggct acatcgtgaa ctggctgaag cag 53 <210> SEQ
ID NO 85 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HF5 <400> SEQUENCE:
85 atcgtgaact ggctgaagca gagccatgga aagaaccttg agtggattgg ac 52
<210> SEQ ID NO 86 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HF6
<400> SEQUENCE: 86 gaaccttgag tggattggac ttattaatcc
atacaaaggt cttactacct ac 52 <210> SEQ ID NO 87 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HR6 <400> SEQUENCE: 87 aatgtggcct
tgcccttgaa tttctggttg taggtagtaa gacctttgta tg 52 <210> SEQ
ID NO 88 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HR5 <400> SEQUENCE:
88 catgtaggct gtgctggatg acttgtctac agttaatgtg gccttgccct tg 52
<210> SEQ ID NO 89 <211> LENGTH: 55 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HR4
<400> SEQUENCE: 89 actgcagagt cttcagatgt cagactgagg
agctccatgt aggctgtgct ggatg 55 <210> SEQ ID NO 90 <211>
LENGTH: 52 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HR3 <400> SEQUENCE: 90 accatagtac
ccagatcttg cacagtaata gactgcagag tcttcagatg tc 52 <210> SEQ
ID NO 91 <211> LENGTH: 52 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HR2 <400> SEQUENCE:
91 gcgccccaga catcgaagta ccagtccgag tcaccatagt acccagatct tg 52
<210> SEQ ID NO 92 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LH-HR1
<400> SEQUENCE: 92 gcgaatactc gaggagacgg tgaccgtggt
ccctgcgccc cagacatcga ag 52 <210> SEQ ID NO 93 <211>
LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HL-HF1 <400> SEQUENCE: 93 gcgtatgaac
cggtgaggtc cagctgcaac agtctggacc tg 42 <210> SEQ ID NO 94
<211> LENGTH: 55 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer - 194-HL-HR1 <400> SEQUENCE: 94
accgccacca gaggagacgg tgaccgtggt ccctgcgccc cagacatcga agtac 55
<210> SEQ ID NO 95 <211> LENGTH: 52 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HL-HR0
<400> SEQUENCE: 95 acctcctcca gatccaccac cgcccgagcc
accgccacca gaggagacgg tg 52 <210> SEQ ID NO 96 <211>
LENGTH: 49 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-HL-LF1 <400> SEQUENCE: 96 gcgggggagg
tggcagtgct agcgacatcc agatgacaca gactacatc 49 <210> SEQ ID NO
97 <211> LENGTH: 34 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer - 194-HL-LR3Xho <400>
SEQUENCE: 97 gcgaatactc gagcgtttgg ttaccagttt ggtg 34 <210>
SEQ ID NO 98 <211> LENGTH: 37 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-HL-LR3Xba
<400> SEQUENCE: 98 gcgatatcta gattaccgtt tggttaccag tttggtg
37 <210> SEQ ID NO 99 <211> LENGTH: 43 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer -
194-HL-HF1R1 <400> SEQUENCE: 99 gcgtatgaga attcagaggt
ccagctgcaa cagtctggac ctg 43 <210> SEQ ID NO 100 <211>
LENGTH: 41 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer - 194-LH-LF1R1 <400> SEQUENCE: 100
gcgtatgaga attctgacat ccagatgaca cagactacat c 41
<210> SEQ ID NO 101 <211> LENGTH: 54 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer - 194-LH-HR1Xba
<400> SEQUENCE: 101 gcgtatctag attaggagac ggtgaccgtg
gtccctgcgc cccagacatc gaag 54 <210> SEQ ID NO 102 <211>
LENGTH: 725 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic polynucleotide - G28-1 VLVH (DNA) <400> SEQUENCE:
102 accggtgaca tccagatgac tcagtctcca gcctccctat ctgcatctgt
gggagagact 60 gtcaccatca catgtcgaac aagtgaaaat gtttacagtt
atttggcttg gtatcagcag 120 aaacagggaa aatctcctca gctcctggtc
tcttttgcaa aaaccttagc agaaggtgtg 180 ccatcaaggt tcagtggcag
tggatcaggc acacagtttt ctctgaagat cagcagcctg 240 cagcctgaag
attctggaag ttatttctgt caacatcatt ccgataatcc gtggacgttc 300
ggtggaggca ccgaactgga gatcaaaggt ggcggtggct cgggcggtgg tgggtcgggt
360 ggcggcggat ctgctagcgc agtccagctg cagcagtctg gacctgagct
ggaaaagcct 420 ggcgcttcag tgaagatttc ctgcaaggct tctggttact
cattcactgg ctacaatatg 480 aactgggtga agcagaataa tggaaagagc
cttgagtgga ttggaaatat tgatccttat 540 tatggtggta ctacctacaa
ccggaagttc aagggcaagg ccacattgac tgtagacaaa 600 tcctccagca
cagcctacat gcagctcaag agtctgacat ctgaggactc tgcagtctat 660
tactgtgcaa gatcggtcgg ccctatggac tactggggtc aaggaacctc agtcaccgtc
720 tcgag 725 <210> SEQ ID NO 103 <211> LENGTH: 239
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polypeptide - G-28-1 VLVH (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(107) <223>
OTHER INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (108)..(124) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (125)..(239) <223> OTHER
INFORMATION: VH <400> SEQUENCE: 103 Asp Ile Gln Met Thr Gln
Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Glu Thr Val Thr
Ile Thr Cys Arg Thr Ser Glu Asn Val Tyr Ser Tyr 20 25 30 Leu Ala
Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 35 40 45
Ser Phe Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly 50
55 60 Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser Ser Leu Gln
Pro 65 70 75 80 Glu Asp Ser Gly Ser Tyr Phe Cys Gln His His Ser Asp
Asn Pro Trp 85 90 95 Thr Phe Gly Gly Gly Thr Glu Leu Glu Ile Lys
Gly Gly Gly Gly Ser 100 105 110 Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ala Ser Ala Val Gln Leu 115 120 125 Gln Gln Ser Gly Pro Glu Leu
Glu Lys Pro Gly Ala Ser Val Lys Ile 130 135 140 Ser Cys Lys Ala Ser
Gly Tyr Ser Phe Thr Gly Tyr Asn Met Asn Trp 145 150 155 160 Val Lys
Gln Asn Asn Gly Lys Ser Leu Glu Trp Ile Gly Asn Ile Asp 165 170 175
Pro Tyr Tyr Gly Gly Thr Thr Tyr Asn Arg Lys Phe Lys Gly Lys Ala 180
185 190 Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu
Lys 195 200 205 Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala
Arg Ser Val 210 215 220 Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser 225 230 235 <210> SEQ ID NO 104 <211>
LENGTH: 767 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic polynucleotide - G28-1 VHVL (DNA) <400> SEQUENCE:
104 accggtgagg tccagctgca acagtctgga cctgaactgg tgaagcctgg
agcttcaatg 60 aagatttcct gcaaggcctc tggttactca ttcactggct
acatcgtgaa ctggctgaag 120 cagagccatg gaaagaacct tgagtggatt
ggacttatta atccatacaa aggtcttact 180 acctacaacc agaaattcaa
gggcaaggcc acattaactg tagacaagtc atccagcaca 240 gcctacatgg
agctcctcag tctgacatct gaagactctg cagtctatta ctgtgcaaga 300
tctgggtact atggtgactc ggactggtac ttcgatgtct ggggcgcagg gaccacggtc
360 accgtctcct ctggtggcgg tggctcgggc ggtggtggat ctggaggagg
tgggagcggg 420 ggaggtggca gtgctagcga catccagatg acacagacta
catcctccct gtctgcctct 480 ctgggagaca gagtcaccat cagttgcagg
gcaagtcagg acattcgcaa ttatttaaac 540 tggtatcagc agaaaccaga
tggaactgtt aaactcctga tctactacac atcaagatta 600 cactcaggag
tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc 660
attgccaacc tgcaaccaga agatattgcc acttactttt gccaacaggg taatacgctt
720 ccgtggacgt tcggtggagg caccaaactg gtaaccaaac gctcgag 767
<210> SEQ ID NO 105 <211> LENGTH: 253 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polypeptide - G28-1 VHVL
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(121) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (122)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(253) <223> OTHER INFORMATION: VL
<400> SEQUENCE: 105 Glu Val Gln Leu Gln Gln Ser Gly Pro Glu
Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Met Lys Ile Ser Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly Tyr 20 25 30 Ile Val Asn Trp Leu Lys
Gln Ser His Gly Lys Asn Leu Glu Trp Ile 35 40 45 Gly Leu Ile Asn
Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe 50 55 60 Lys Gly
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80
Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val
Trp 100 105 110 Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
Gly Ser Gly 115 120 125 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
Gly Gly Ser Ala Ser 130 135 140 Asp Ile Gln Met Thr Gln Thr Thr Ser
Ser Leu Ser Ala Ser Leu Gly 145 150 155 160 Asp Arg Val Thr Ile Ser
Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 165 170 175 Leu Asn Trp Tyr
Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 180 185 190 Tyr Tyr
Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 195 200 205
Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro 210
215 220 Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro
Trp 225 230 235 240 Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg
Ser 245 250 <210> SEQ ID NO 106 <211> LENGTH: 749
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide - G19-4 VLVH (DNA) <400> SEQUENCE: 106
accggtgaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga
60 gtcaccatca gttgcagggc aagtcaggac attcgcaatt atttaaactg
gtatcagcag 120 aaaccagatg gaactgttaa actcctgatc tactacacat
caagattaca ctcaggagtc 180 ccatcaaggt tcagtggcag tgggtctgga
acagattatt ctctcaccat tgccaacctg 240 caaccagaag atattgccac
ttacttttgc caacagggta atacgcttcc gtggacgttc 300 ggtggaggca
ccaaactggt aaccaaacgg ggtggcggtg gctcgggcgg tggtggatct 360
ggaggaggtg ggagcgctag cgaggtccag ctgcaacagt ctggacctga actggtgaag
420 cctggagctt caatgaagat ttcctgcaag gcctctggtt actcattcac
tggctacatc 480
gtgaactggc tgaagcagag ccatggaaag aaccttgagt ggattggact tattaatcca
540 tacaaaggtc ttactaccta caaccagaaa ttcaagggca aggccacatt
aactgtagac 600 aagtcatcca gcacagccta catggagctc ctcagtctga
catctgaaga ctctgcagtc 660 tattactgtg caagatctgg gtactatggt
gactcggact ggtacttcga tgtctggggc 720 gcagggacca cggtcaccgt
ctcctcgag 749 <210> SEQ ID NO 107 <211> LENGTH: 247
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polypeptide - G-19-4 VLVH (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(108) <223>
OTHER INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (109)..(125) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (126)..(247) <223> OTHER
INFORMATION: VH <400> SEQUENCE: 107 Asp Ile Gln Met Thr Gln
Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 1 5 10 15 Asp Arg Val Thr
Ile Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 20 25 30 Leu Asn
Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 35 40 45
Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50
55 60 Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln
Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr
Leu Pro Trp 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys
Arg Gly Gly Gly Gly 100 105 110 Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Ala Ser Glu Val Gln 115 120 125 Leu Gln Gln Ser Gly Pro Glu
Leu Val Lys Pro Gly Ala Ser Met Lys 130 135 140 Ile Ser Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly Tyr Ile Val Asn 145 150 155 160 Trp Leu
Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile 165 170 175
Asn Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys 180
185 190 Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu
Leu 195 200 205 Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys
Ala Arg Ser 210 215 220 Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp
Val Trp Gly Ala Gly 225 230 235 240 Thr Thr Val Thr Val Ser Ser 245
<210> SEQ ID NO 108 <211> LENGTH: 752 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic polynucleotide - G19-4
VHVL (DNA) <400> SEQUENCE: 108 accggtgagg tccagctgca
acagtctgga cctgaactgg tgaagcctgg agcttcaatg 60 aagatttcct
gcaaggcctc tggttactca ttcactggct acatcgtgaa ctggctgaag 120
cagagccatg gaaagaacct tgagtggatt ggacttatta atccatacaa aggtcttact
180 acctacaacc agaaattcaa gggcaaggcc acattaactg tagacaagtc
atccagcaca 240 gcctacatgg agctcctcag tctgacatct gaagactctg
cagtctatta ctgtgcaaga 300 tctgggtact atggtgactc ggactggtac
ttcgatgtct ggggcgcagg gaccacggtc 360 accgtctcct ctggtggcgg
tggctcgggc ggtggtggat ctggaggagg tgggagcgct 420 agcgacatcc
agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc 480
accatcagtt gcagggcaag tcaggacatt cgcaattatt taaactggta tcagcagaaa
540 ccagatggaa ctgttaaact cctgatctac tacacatcaa gattacactc
aggagtccca 600 tcaaggttca gtggcagtgg gtctggaaca gattattctc
tcaccattgc caacctgcaa 660 ccagaagata ttgccactta cttttgccaa
cagggtaata cgcttccgtg gacgttcggt 720 ggaggcacca aactggtaac
caaacgctcg ag 752 <210> SEQ ID NO 109 <211> LENGTH: 248
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polypeptide - G19-4 VHVL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(122) <223>
OTHER INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (123)..(139) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (140)..(248) <223> OTHER
INFORMATION: VL <400> SEQUENCE: 109 Glu Val Gln Leu Gln Gln
Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Met Lys Ile
Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 20 25 30 Ile Val
Asn Trp Leu Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile 35 40 45
Gly Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe 50
55 60 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 65 70 75 80 Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Tyr Cys 85 90 95 Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp
Tyr Phe Asp Val Trp 100 105 110 Gly Ala Gly Thr Thr Val Thr Val Ser
Ser Gly Gly Gly Gly Ser Gly 115 120 125 Gly Gly Gly Ser Gly Gly Gly
Gly Ser Ala Ser Asp Ile Gln Met Thr 130 135 140 Gln Thr Thr Ser Ser
Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 145 150 155 160 Ser Cys
Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln 165 170 175
Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Arg 180
185 190 Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly
Thr 195 200 205 Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu Asp
Ile Ala Thr 210 215 220 Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp
Thr Phe Gly Gly Gly 225 230 235 240 Thr Lys Leu Val Thr Lys Arg Ser
245 <210> SEQ ID NO 110 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer
<220> FEATURE: <221> NAME/KEY: misc_feature <223>
OTHER INFORMATION: ssc(s)-hIgG1 (DNA) <400> SEQUENCE: 110
gagcccaaat cttctgacaa aactcacaca tctccaccgt gctca 45 <210>
SEQ ID NO 111 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide - ssc(s)-hIgG1
(AA) <400> SEQUENCE: 111 Glu Pro Lys Ser Ser Asp Lys Thr His
Thr Ser Pro Pro Cys Ser 1 5 10 15 <210> SEQ ID NO 112
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: scc(s)-hIgG1
(DNA) <400> SEQUENCE: 112 gagcccaaat cttctgacaa aactcacaca
tgtccaccgt gctca 45 <210> SEQ ID NO 113 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
peptide - scc(s)-hIgG1 (AA) <400> SEQUENCE: 113 Glu Pro Lys
Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Ser 1 5 10 15
<210> SEQ ID NO 114 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
css(s)-hIgG1 (DNA) <400> SEQUENCE: 114 gagcccaaat cttgtgacaa
aactcacaca tctccaccga gctca 45 <210> SEQ ID NO 115
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic peptide - css(s)-hIgG1 (AA) <400>
SEQUENCE: 115 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro
Ser Ser 1 5 10 15 <210> SEQ ID NO 116 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
polynucleotide <220> FEATURE: <221> NAME/KEY:
misc_feature <223> OTHER INFORMATION: scs(s)-hIgG1 (DNA)
<400> SEQUENCE: 116 gagcccaaat cttgtgacaa aactcacaca
tgtccaccga gctca 45 <210> SEQ ID NO 117 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
peptide - scs(s)-hIgG1 (AA) <400> SEQUENCE: 117 Glu Pro Lys
Ser Ser Asp Lys Thr His Thr Cys Pro Pro Ser Ser 1 5 10 15
<210> SEQ ID NO 118 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <400>
SEQUENCE: 118 gagcccaaat cttgtgacaa aactcacaca tgtccaccgt gctca 45
<210> SEQ ID NO 119 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <400>
SEQUENCE: 119 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro
Cys Ser 1 5 10 15 <210> SEQ ID NO 120 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer <400> SEQUENCE: 120 gagcccaaat cttgtgacaa aactcacaca
tgtccaccgt gccca 45 <210> SEQ ID NO 121 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <400> SEQUENCE: 121 Glu Pro Lys Ser Cys Asp Lys Thr
His Thr Cys Pro Pro Cys Pro 1 5 10 15 <210> SEQ ID NO 122
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <400> SEQUENCE: 122 gagcccaaat
cttctgacaa aactcacaca tctccaccga gccca 45 <210> SEQ ID NO 123
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <400> SEQUENCE: 123 Glu
Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro 1 5 10 15
<210> SEQ ID NO 124 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: csc(p)-hIgG1 (DNA) <400> SEQUENCE: 124
gagcccaaat cttgtgacaa aactcacaca tctccaccgt gccca 45 <210>
SEQ ID NO 125 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: csc(p)-hIgG1 (AA) <400> SEQUENCE: 125 Glu Pro
Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 126 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (DNA) <400> SEQUENCE: 126
gagcccaaat cttctgacaa aactcacaca tctccaccgt gccca 45 <210>
SEQ ID NO 127 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (AA) <400> SEQUENCE: 127 Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 128 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: scc(p)-hIgG1 (DNA) <400> SEQUENCE: 128
gagcccaaat cttctgacaa aactcacaca tgtccaccgt gccca 45 <210>
SEQ ID NO 129 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: scc(p)-hIgG1 (AA) <400> SEQUENCE: 129 Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15
<210> SEQ ID NO 130 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: css(p)-hIgG1 (DNA) <400> SEQUENCE: 130
gagcccaaat cttgtgacaa aactcacaca tctccaccga gccca 45 <210>
SEQ ID NO 131 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: css(p)-hIgG1 (AA) <400> SEQUENCE: 131 Glu Pro
Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Ser Pro 1 5 10 15
<210> SEQ ID NO 132 <211> LENGTH: 45 <212> TYPE:
DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (DNA) <400> SEQUENCE: 132
gagcccaaat cttgtgacaa aactcacaca tgtccaccga gccca 45 <210>
SEQ ID NO 133 <211> LENGTH: 15 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: ssc(p)-hIgG1 (DNA) <400> SEQUENCE: 133 Glu Pro
Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Ser Pro 1 5 10 15
<210> SEQ ID NO 134 <211> LENGTH: 18 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
ssc(p) (DNA) <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <400> SEQUENCE: 134 agttgtccac cgtgccca 18
<210> SEQ ID NO 135 <211> LENGTH: 6 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <400>
SEQUENCE: 135 Ser Cys Pro Pro Cys Pro 1 5 <210> SEQ ID NO 136
<211> LENGTH: 7 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <400> SEQUENCE: 136 tgatcag 7
<210> SEQ ID NO 137 <211> LENGTH: 2 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide <400>
SEQUENCE: 137 Asp Gln 1 <210> SEQ ID NO 138 <211>
LENGTH: 7 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic primer <400> SEQUENCE: 138 ctcgagt 7 <210>
SEQ ID NO 139 <211> LENGTH: 2 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide <400>
SEQUENCE: 139 Ser Ser 1 <210> SEQ ID NO 140 <211>
LENGTH: 654 <212> TYPE: DNA <213> ORGANISM: Homo
sapiens <220> FEATURE: <223> OTHER INFORMATION: hIgG1
wild type <400> SEQUENCE: 140 gcacctgaac tcctgggtgg
accgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60 ctcatgatct
cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120
cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag
180 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac
cgtcctgcac 240 caggactggc tgaatggcaa ggagtacaag tgcaaggtct
ccaacaaagc cctcccagcc 300 cccatcgaga aaacaatctc caaagccaaa
gggcagcccc gagaaccaca ggtgtacacc 360 ctgcccccat cccgggatga
gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420 ggcttctatc
ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac 480
tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc
540 accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt
gatgcatgag 600 gctctgcaca accactacac gcagaagagc ctctccctgt
ctccgggtaa atga 654 <210> SEQ ID NO 141 <211> LENGTH:
217 <212> TYPE: PRT <213> ORGANISM: Homo sapiens
<220> FEATURE: <223> OTHER INFORMATION: hIgG1 wild type
<400> SEQUENCE: 141 Ala Pro Glu Leu Leu Gly Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp Thr Leu Met Ile Ser
Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val Val Asp Val Ser His
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 Val Asp Gly Val
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55 60 Gln Tyr
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 65 70 75 80
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 85
90 95 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
Gln 100 105 110 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu 115 120 125 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro 130 135 140 Ser Asp Ile Ala Val Glu Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 Tyr Lys Thr Thr Pro Pro
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175 Tyr Ser Lys Leu
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185 190 Phe Ser
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 195 200 205
Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215 <210> SEQ ID NO
142 <211> LENGTH: 654 <212> TYPE: DNA <213>
ORGANISM: Homo sapiens <220> FEATURE: <223> OTHER
INFORMATION: hIgG1 (P238S) <400> SEQUENCE: 142 gcacctgaac
tcctgggtgg atcgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60
ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac
120 cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc
caagacaaag 180 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca
gcgtcctcac cgtcctgcac 240 caggactggc tgaatggcaa ggagtacaag
tgcaaggtct ccaacaaagc cctcccagcc 300 cccatcgaga aaacaatctc
caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 360 ctgcccccat
cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420
ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac
480 tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta
cagcaagctc 540 accgtggaca agagcaggtg gcagcagggg aacgtcttct
catgctccgt gatgcatgag 600 gctctgcaca accactacac gcagaagagc
ctctccctgt ctccgggtaa atga 654 <210> SEQ ID NO 143
<211> LENGTH: 217 <212> TYPE: PRT <213> ORGANISM:
Homo sapiens <220> FEATURE: <223> OTHER INFORMATION:
hIgG1 (P238S) <400> SEQUENCE: 143 Ala Pro Glu Leu Leu Gly Gly
Ser Ser Val Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp Thr Leu
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val Val Asp
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 Val
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55
60 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
65 70 75 80 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys 85 90 95 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln 100 105 110
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 115
120 125 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
Pro 130 135 140 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
Glu Asn Asn 145 150 155 160 Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu 165 170 175 Tyr Ser Lys Leu Thr Val Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val 180 185 190 Phe Ser Cys Ser Val Met
His Glu Ala Leu His Asn His Tyr Thr Gln 195 200 205 Lys Ser Leu Ser
Leu Ser Pro Gly Lys 210 215 <210> SEQ ID NO 144 <211>
LENGTH: 654 <212> TYPE: DNA <213> ORGANISM: Homo
sapiens <220> FEATURE: <223> OTHER INFORMATION: hIgG1
(P331S) <400> SEQUENCE: 144 gcacctgaac tcctgggtgg accgtcagtc
ttcctcttcc ccccaaaacc caaggacacc 60 ctcatgatct cccggacccc
tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120 cctgaggtca
agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag 180
ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac
240 caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc
cctcccagcc 300 tccatcgaga aaacaatctc caaagccaaa gggcagcccc
gagaaccaca ggtgtacacc 360 ctgcccccat cccgggatga gctgaccaag
aaccaggtca gcctgacctg cctggtcaaa 420 ggcttctatc ccagcgacat
cgccgtggag tgggagagca atgggcagcc ggagaacaac 480 tacaagacca
cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc 540
accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag
600 gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atga 654
<210> SEQ ID NO 145 <211> LENGTH: 217 <212> TYPE:
PRT <213> ORGANISM: Homo sapiens <220> FEATURE:
<223> OTHER INFORMATION: hIgG1 (P331S) <400> SEQUENCE:
145 Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
1 5 10 15 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
Cys Val 20 25 30 Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
Phe Asn Trp Tyr 35 40 45 Val Asp Gly Val Glu Val His Asn Ala Lys
Thr Lys Pro Arg Glu Glu 50 55 60 Gln Tyr Asn Ser Thr Tyr Arg Val
Val Ser Val Leu Thr Val Leu His 65 70 75 80 Gln Asp Trp Leu Asn Gly
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 85 90 95 Ala Leu Pro Ala
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 100 105 110 Pro Arg
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 115 120 125
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 130
135 140 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
Asn 145 150 155 160 Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
Ser Phe Phe Leu 165 170 175 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg
Trp Gln Gln Gly Asn Val 180 185 190 Phe Ser Cys Ser Val Met His Glu
Ala Leu His Asn His Tyr Thr Gln 195 200 205 Lys Ser Leu Ser Leu Ser
Pro Gly Lys 210 215 <210> SEQ ID NO 146 <211> LENGTH:
654 <212> TYPE: DNA <213> ORGANISM: Homo sapiens
<220> FEATURE: <223> OTHER INFORMATION: hIgG1
(P238S/P331S) <400> SEQUENCE: 146 gcacctgaac tcctgggtgg
atcgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60 ctcatgatct
cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120
cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag
180 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac
cgtcctgcac 240 caggactggc tgaatggcaa ggagtacaag tgcaaggtct
ccaacaaagc cctcccagcc 300 tccatcgaga aaacaatctc caaagccaaa
gggcagcccc gagaaccaca ggtgtacacc 360 ctgcccccat cccgggatga
gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420 ggcttctatc
ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac 480
tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc
540 accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt
gatgcatgag 600 gctctgcaca accactacac gcagaagagc ctctccctgt
ctccgggtaa atga 654 <210> SEQ ID NO 147 <211> LENGTH:
217 <212> TYPE: PRT <213> ORGANISM: Homo sapiens
<220> FEATURE: <223> OTHER INFORMATION: hIgG1
(P238S/P331S) <400> SEQUENCE: 147 Ala Pro Glu Leu Leu Gly Gly
Ser Ser Val Phe Leu Phe Pro Pro Lys 1 5 10 15 Pro Lys Asp Thr Leu
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30 Val Val Asp
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 Val
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55
60 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
65 70 75 80 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys 85 90 95 Ala Leu Pro Ala Ser Ile Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln 100 105 110 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
Pro Ser Arg Asp Glu Leu 115 120 125 Thr Lys Asn Gln Val Ser Leu Thr
Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140 Ser Asp Ile Ala Val Glu
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 Tyr Lys Thr
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175 Tyr
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185
190 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
195 200 205 Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215 <210>
SEQ ID NO 148 <211> LENGTH: 60 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <223> OTHER INFORMATION: STD1 (DNA) <400>
SEQUENCE: 148 aattatggtg gcggtggctc gggcggtggt ggatctggag
gaggtgggag tgggaattct 60 <210> SEQ ID NO 149 <211>
LENGTH: 20 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion linker region <220> FEATURE: <223> OTHER
INFORMATION: STD1 (AA) <400> SEQUENCE: 149 Asn Tyr Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 1 5 10 15 Ser Gly
Asn Ser 20 <210> SEQ ID NO 150 <211> LENGTH: 114
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer <220> FEATURE: <223> OTHER INFORMATION: STD2
(DNA) <400> SEQUENCE: 150 aattatggtg gcggtggctc gggcggtggt
ggatctggag gaggtgggag tgggaattat 60 ggtggcggtg gctcgggcgg
tggtggatct ggaggaggtg ggagtgggaa ttct 114 <210> SEQ ID NO 151
<211> LENGTH: 38 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <220> FEATURE:
<223> OTHER INFORMATION: STD2 (AA) <400> SEQUENCE: 151
Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
1 5 10 15 Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Gly Gly 20 25 30 Gly Gly Ser Gly Asn Ser 35 <210> SEQ ID NO
152 <211> LENGTH: 6 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer <220> FEATURE:
<223> OTHER INFORMATION: Linker H1 (PN) <400> SEQUENCE:
152 aattct 6 <210> SEQ ID NO 153 <211> LENGTH: 2
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
region <220> FEATURE: <223> OTHER INFORMATION: Linker
H1 (AA) <400> SEQUENCE: 153 Asn Ser 1 <210> SEQ ID NO
154 <211> LENGTH: 24 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer <220> FEATURE:
<223> OTHER INFORMATION: Linker H2 (PN) <400> SEQUENCE:
154 ggtggcggtg gctcggggaa ttct 24 <210> SEQ ID NO 155
<211> LENGTH: 8 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <220> FEATURE:
<223> OTHER INFORMATION: Linker H2 (AA) <400> SEQUENCE:
155 Gly Gly Gly Gly Ser Gly Asn Ser 1 5 <210> SEQ ID NO 156
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthetic primer <220> FEATURE: <223>
OTHER INFORMATION: Linker H3 (PN) <400> SEQUENCE: 156
aattatggtg gcggtggctc tgggaattct 30 <210> SEQ ID NO 157
<211> LENGTH: 10 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <220> FEATURE:
<223> OTHER INFORMATION: Linker H3 (AA) <400> SEQUENCE:
157 Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser 1 5 10 <210> SEQ
ID NO 158 <211> LENGTH: 39 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer <220> FEATURE:
<223> OTHER INFORMATION: Linker H4 (PN) <400> SEQUENCE:
158 ggtggcggtg gctcgggcgg tggtggatct gggaattct 39 <210> SEQ
ID NO 159 <211> LENGTH: 13 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion linker region <220> FEATURE:
<223> OTHER INFORMATION: Linker H4 (AA) <400> SEQUENCE:
159 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser 1 5 10
<210> SEQ ID NO 160 <211> LENGTH: 45 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic primer <220>
FEATURE: <223> OTHER INFORMATION: Linker H5 (PN) <400>
SEQUENCE: 160 aattatggtg gcggtggctc gggcggtggt ggatctggga attct 45
<210> SEQ ID NO 161 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker H5 (AA) <400>
SEQUENCE: 161 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Asn Ser 1 5 10 15 <210> SEQ ID NO 162 <211> LENGTH: 54
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
primer <220> FEATURE: <223> OTHER INFORMATION: Linker
H6 (PN) <400> SEQUENCE: 162 ggtggcggtg gctcgggcgg tggtggatct
gggggaggag gcagcgggaa ttct 54 <210> SEQ ID NO 163 <211>
LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion linker region <220> FEATURE: <223> OTHER
INFORMATION: Linker H6 (AA) <400> SEQUENCE: 163 Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 1 5 10 15 Asn
Ser <210> SEQ ID NO 164 <211> LENGTH: 24 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer
<220> FEATURE: <223> OTHER INFORMATION: Linker H7 (PN)
<400> SEQUENCE: 164 gggtgtccac cttgtccgaa ttct 24 <210>
SEQ ID NO 165 <211> LENGTH: 8 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker H7 (AA) <400>
SEQUENCE: 165 Gly Cys Pro Pro Cys Pro Asn Ser 1 5 <210> SEQ
ID NO 166 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthetic primer <220> FEATURE:
<223> OTHER INFORMATION: Linker(G4S)3 <400> SEQUENCE:
166 ggtggcggtg gatccggcgg aggtgggtcg ggtggcggcg gatct 45
<210> SEQ ID NO 167 <211> LENGTH: 12 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion linker region <220>
FEATURE: <223> OTHER INFORMATION: Linker(G4S)3 <400>
SEQUENCE: 167 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 1 5
10 <210> SEQ ID NO 168 <211> LENGTH: 60 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthetic primer
<220> FEATURE: <223> OTHER INFORMATION:
Linker(G4S)4
<400> SEQUENCE: 168 ggtggcggtg gctcgggcgg tggtggatct
ggaggaggtg ggagcggggg aggtggcagt 60 <210> SEQ ID NO 169
<211> LENGTH: 17 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker region <220> FEATURE:
<223> OTHER INFORMATION: Linker(G4S)4 <400> SEQUENCE:
169 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly
1 5 10 15 Ser <210> SEQ ID NO 170 <211> LENGTH: 2337
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-STD1-2e12HL (DNA) <400> SEQUENCE: 170 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagctcagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt
1560 gggaattctc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc
ctcacagagc 1620 ctgtccatca catgcaccgt ctcagggttc tcattaaccg
gctatggtgt aaactgggtt 1680 cgccagcctc caggaaaggg tctggagtgg
ctgggaatga tatggggtga tggaagcaca 1740 gactataatt cagctctcaa
atccagacta tcgatcacca aggacaactc caagagccaa 1800 gttttcttaa
aaatgaacag tctgcaaact gatgacacag ccagatacta ctgtgctcga 1860
gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc
1920 accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg
tgggtcgggt 1980 ggcggcggat ctgacattgt gctcacccaa tctccagctt
ctttggctgt gtctctaggt 2040 cagagagcca ccatctcctg cagagccagt
gaaagtgttg aatattatgt cacaagttta 2100 atgcagtggt accaacagaa
accaggacag ccacccaaac tcctcatctc tgctgctagc 2160 aacgtagaat
ctggggtccc tgccaggttt agtggcagtg ggtctgggac agactttagc 2220
ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca gcaaagtagg
2280 aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgtta atctaga
2337 <210> SEQ ID NO 171 <211> LENGTH: 772 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2H7sssIgG1-STD1-2e12HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(281) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(519) <223> OTHER INFORMATION: EFD-BD2 linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (520)..(640) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (641)..(660) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (661)..(772) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 171 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510
Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro 515
520 525 Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val
Ser 530 535 540 Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg
Gln Pro Pro 545 550 555 560 Gly Lys Gly Leu Glu Trp Leu Gly Met Ile
Trp Gly Asp Gly Ser Thr 565 570 575 Asp Tyr Asn Ser Ala Leu Lys Ser
Arg Leu Ser Ile Thr Lys Asp Asn 580 585 590 Ser Lys Ser Gln Val Phe
Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 595 600 605 Thr Ala Arg Tyr
Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 610 615 620 Tyr Val
Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 625 630 635
640 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
645 650 655 Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser
Leu Ala 660 665 670 Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg
Ala Ser Glu Ser 675 680 685 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln
Trp Tyr Gln Gln Lys Pro 690 695 700 Gly Gln Pro Pro Lys Leu Leu Ile
Ser Ala Ala Ser Asn Val Glu Ser 705 710 715 720 Gly Val Pro Ala Arg
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 725 730 735 Leu Asn Ile
His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 740 745 750 Gln
Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 755 760
765 Glu Ile Lys Arg 770 <210> SEQ ID NO 172 <211>
LENGTH: 772 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1(P238S/P331S)-STD1-2e12HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(519) <223> OTHER INFORMATION: EFD-BD2 linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (520)..(640) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (641)..(660) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (661)..(772) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 172 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Ser Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Gln Val
Gln Leu Lys Glu Ser Gly Pro 515 520 525 Gly Leu Val Ala Pro Ser Gln
Ser Leu Ser Ile Thr Cys Thr Val Ser 530 535 540 Gly Phe Ser Leu Thr
Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 545 550 555 560 Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 565 570 575
Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 580
585 590 Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp
Asp 595 600 605 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn
Phe His Tyr 610 615 620 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Gly 645 650 655 Gly Gly Gly Ser Asp Ile
Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 660 665 670 Val Ser Leu Gly
Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 675 680 685 Val Glu
Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 690 695 700
Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 705
710 715 720 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp
Phe Ser 725 730 735 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala
Met Tyr Phe Cys 740 745 750 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe
Gly Gly Gly Thr Lys Leu 755 760 765 Glu Ile Lys Arg 770 <210>
SEQ ID NO 173 <211> LENGTH: 2322 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-STD1-2e12LH (DNA)
<400> SEQUENCE: 173 aagcttgccg ccatggattt tcaagtgcag
attttcagct tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca
aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga
aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180
taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct
240 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc
tctcacaatc 300 agcagagtgg aggctgaaga tgctgccact tattactgcc
agcagtggag ttttaaccca 360 cccacgttcg gtgctgggac caagctggag
ctgaaagatg gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag
ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg
cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540
aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat
600 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac
actgactgta 660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc
tgacatctga agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat
agtaactctt actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac
cgtctctgat caggagccca aatcttctga caaaactcac 840 acatccccac
cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900
ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg
960 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
cgtggaggtg 1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca
gcacgtaccg tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg
aatggcaagg agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc
catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg
tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260
ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat
1320 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
cggctccttc 1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc
agcaggggaa cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac
cactacacgc agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg
cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560 gggaattctg
acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 1620
agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg
1680 cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc
tgctagcaac 1740 gtagaatctg gggtccctgc caggtttagt ggcagtgggt
ctgggacaga ctttagcctc 1800 aacatccatc ctgtggagga ggatgatatt
gcaatgtatt tctgtcagca aagtaggaag 1860 gttccatgga cgttcggtgg
aggcaccaag ctggaaatca aacggggtgg cggtggatcc 1920 ggcggaggtg
ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 1980
ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc
2040 ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg
gctgggaatg 2100 atatggggtg atggaagcac agactataat tcagctctca
aatccagact atcgatcacc 2160 aaggacaact ccaagagcca agttttctta
aaaatgaaca gtctgcaaac tgatgacaca 2220 gccagatact actgtgctcg
agatggttat agtaactttc attactatgt tatggactac 2280 tggggtcaag
gaacctcagt caccgtctcc tcttaatcta ga 2322 <210> SEQ ID NO 174
<211> LENGTH: 767 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: 2H7sssIgG1-STD1-2e12LH (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(519) <223> OTHER INFORMATION: EFD-BD2 linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (520)..(631) <223> OTHER INFORMATION: VL2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (632)..(646) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (647)..(767) <223> OTHER INFORMATION: VH2
<400> SEQUENCE: 174 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Asp Ile
Val Leu Thr Gln Ser Pro Ala 515 520 525 Ser Leu Ala Val Ser Leu Gly
Gln Arg Ala Thr Ile Ser Cys Arg Ala 530 535 540 Ser Glu Ser Val Glu
Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 545 550 555 560 Gln Lys
Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 565 570 575
Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 580
585 590 Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala
Met 595 600 605 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe
Gly Gly Gly 610 615 620 Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly
Ser Gly Gly Gly Gly 625 630 635 640
Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 645
650 655 Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser
Gly 660 665 670 Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln
Pro Pro Gly 675 680 685 Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly
Asp Gly Ser Thr Asp 690 695 700 Tyr Asn Ser Ala Leu Lys Ser Arg Leu
Ser Ile Thr Lys Asp Asn Ser 705 710 715 720 Lys Ser Gln Val Phe Leu
Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 725 730 735 Ala Arg Tyr Tyr
Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr 740 745 750 Val Met
Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 755 760 765
<210> SEQ ID NO 175 <211> LENGTH: 767 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1(P238S/P331S)-STD1-2e12LH
(w/2e12 leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(282) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(519) <223> OTHER
INFORMATION: EFD-BD2 Linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (520)..(631)
<223> OTHER INFORMATION: VL2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (632)..(646)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(647)..(767) <223> OTHER INFORMATION: VH2 <400>
SEQUENCE: 175 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Asp Ile Val Leu Thr
Gln Ser Pro Ala 515 520 525 Ser Leu Ala Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala 530 535 540 Ser Glu Ser Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln 545 550 555 560 Gln Lys Pro Gly Gln
Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 565 570 575 Val Glu Ser
Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 580 585 590 Asp
Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met 595 600
605 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly
610 615 620 Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly
Gly Gly 625 630 635 640 Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys
Glu Ser Gly Pro Gly 645 650 655 Leu Val Ala Pro Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser Gly 660 665 670 Phe Ser Leu Thr Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro Gly 675 680 685 Lys Gly Leu Glu Trp
Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 690 695 700 Tyr Asn Ser
Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 705 710 715 720
Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 725
730 735 Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
Tyr 740 745 750 Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 755 760 765 <210> SEQ ID NO 176 <211> LENGTH:
2376 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-STD2-2e12LH (DNA) <400> SEQUENCE: 176 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt
1560 gggaattatg gtggcggtgg ctcgggcggt ggtggatctg gaggaggtgg
gagtgggaat 1620 tctgacattg tgctcaccca atctccagct tctttggctg
tgtctctagg tcagagagcc 1680 accatctcct gcagagccag tgaaagtgtt
gaatattatg tcacaagttt aatgcagtgg 1740 taccaacaga aaccaggaca
gccacccaaa ctcctcatct ctgctgctag caacgtagaa 1800 tctggggtcc
ctgccaggtt tagtggcagt gggtctggga cagactttag cctcaacatc 1860
catcctgtgg aggaggatga tattgcaatg tatttctgtc agcaaagtag gaaggttcca
1920 tggacgttcg gtggaggcac caagctggaa atcaaacggg gtggcggtgg
atccggcgga 1980 ggtgggtcgg gtggcggcgg atctcaggtg cagctgaagg
agtcaggacc tggcctggtg 2040 gcgccctcac agagcctgtc catcacatgc
accgtctcag ggttctcatt aaccggctat 2100 ggtgtaaact gggttcgcca
gcctccagga aagggtctgg agtggctggg aatgatatgg 2160 ggtgatggaa
gcacagacta taattcagct ctcaaatcca gactatcgat caccaaggac 2220
aactccaaga gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccaga
2280 tactactgtg ctcgagatgg ttatagtaac tttcattact atgttatgga
ctactggggt 2340 caaggaacct cagtcaccgt ctcctcttaa tctaga 2376
<210> SEQ ID NO 177 <211> LENGTH: 785 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-STD2-2e12LH (w/2e12
leader) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(22) <223> OTHER INFORMATION:
Leader <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (23)..(129) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (130)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(537) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (538)..(649) <223> OTHER INFORMATION:
VL2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (650)..(664) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (665)..(785) <223> OTHER INFORMATION:
VH2 <400> SEQUENCE: 177 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440
445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys
Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr
Gly Gly Gly Gly Ser Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly
Ser Gly Asn Ser Asp Ile Val Leu Thr Gln Ser 530 535 540 Pro Ala Ser
Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 545 550 555 560
Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp 565
570 575 Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala
Ala 580 585 590 Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly
Ser Gly Ser 595 600 605 Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val
Glu Glu Asp Asp Ile 610 615 620 Ala Met Tyr Phe Cys Gln Gln Ser Arg
Lys Val Pro Trp Thr Phe Gly 625 630 635 640 Gly Gly Thr Lys Leu Glu
Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly 645 650 655 Gly Gly Ser Gly
Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly 660 665 670 Pro Gly
Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val 675 680 685
Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 690
695 700 Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly
Ser 705 710 715 720 Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser
Ile Thr Lys Asp 725 730 735 Asn Ser Lys Ser Gln Val Phe Leu Lys Met
Asn Ser Leu Gln Thr Asp 740 745 750 Asp Thr Ala Arg Tyr Tyr Cys Ala
Arg Asp Gly Tyr Ser Asn Phe His 755 760 765 Tyr Tyr Val Met Asp Tyr
Trp Gly Gln Gly Thr Ser Val Thr Val Ser 770 775 780 Ser 785
<210> SEQ ID NO 178 <211> LENGTH: 785 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1(P238S/P331S)-STD2-2e12LH
(w/2e12 leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(281) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(537) <223> OTHER
INFORMATION: EFD-BD2 Linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (538)..(649)
<223> OTHER INFORMATION: VL2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (650)..(664)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(665)..(785) <223> OTHER INFORMATION: VH2 <400>
SEQUENCE: 178 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr Gly Gly Gly Gly Ser
Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly Ser Gly Asn Ser Asp
Ile Val Leu Thr Gln Ser 530 535 540 Pro Ala Ser Leu Ala Val Ser Leu
Gly Gln Arg Ala Thr Ile Ser Cys 545 550 555 560 Arg Ala Ser Glu Ser
Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp 565 570 575 Tyr Gln Gln
Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala 580 585 590 Ser
Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser 595 600
605 Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile
610 615 620 Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr
Phe Gly 625 630 635 640 Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly
Gly Gly Ser Gly Gly 645 650 655 Gly Gly Ser Gly Gly Gly Gly Ser Gln
Val Gln Leu Lys Glu Ser Gly 660 665 670 Pro Gly Leu Val Ala Pro Ser
Gln Ser Leu Ser Ile Thr Cys Thr Val 675 680 685 Ser Gly Phe Ser Leu
Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 690 695 700 Pro Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser 705 710 715 720
Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp 725
730 735 Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr
Asp 740 745 750 Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser
Asn Phe His 755 760 765 Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr
Ser Val Thr Val Ser 770 775 780 Ser 785 <210> SEQ ID NO 179
<211> LENGTH: 2391 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-STD2-2e12HL (DNA) <400>
SEQUENCE: 179 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgagcccagc
acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg cggtggctcg
ggcggtggtg gatctggagg aggtgggagt 1560 gggaattatg gtggcggtgg
ctcgggcggt ggtggatctg gaggaggtgg gagtgggaat 1620 tctcaggtgc
agctgaagga gtcaggacct ggcctggtgg cgccctcaca gagcctgtcc 1680
atcacatgca ccgtctcagg gttctcatta accggctatg gtgtaaactg ggttcgccag
1740 cctccaggaa agggtctgga gtggctggga atgatatggg gtgatggaag
cacagactat 1800 aattcagctc tcaaatccag actatcgatc accaaggaca
actccaagag ccaagttttc 1860 ttaaaaatga acagtctgca aactgatgac
acagccagat actactgtgc tcgagatggt 1920 tatagtaact ttcattacta
tgttatggac tactggggtc aaggaacctc agtcaccgtc 1980 tcctctgggg
gtggaggctc tggtggcggt ggatccggcg gaggtgggtc gggtggcggc 2040
ggatctgaca ttgtgctcac ccaatctcca gcttctttgg ctgtgtctct aggtcagaga
2100 gccaccatct cctgcagagc cagtgaaagt gttgaatatt atgtcacaag
tttaatgcag 2160 tggtaccaac agaaaccagg acagccaccc aaactcctca
tctctgctgc tagcaacgta 2220 gaatctgggg tccctgccag gtttagtggc
agtgggtctg ggacagactt tagcctcaac 2280 atccatcctg tggaggagga
tgatattgca atgtatttct gtcagcaaag taggaaggtt 2340 ccatggacgt
tcggtggagg caccaagctg gaaatcaaac gttaatctag a 2391 <210> SEQ
ID NO 180 <211> LENGTH: 790 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-STD2-2e12HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(537) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (538)..(658) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (659)..(678) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (679)..(790) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 180 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr Gly Gly
Gly Gly Ser Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln Leu Lys Glu Ser 530 535 540 Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 545 550 555 560 Val Ser
Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 565 570 575
Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly 580
585 590 Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys 595 600 605 Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr 610 615 620 Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser Asn Phe 625 630 635 640 His Tyr Tyr Val Met Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val 645 650 655 Ser Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 660 665 670 Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 675 680 685 Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser 690 695 700
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln 705
710 715 720 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser
Asn Val 725 730 735 Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly
Ser Gly Thr Asp 740 745 750 Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp Ile Ala Met Tyr 755 760 765 Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe Gly Gly Gly Thr 770 775 780 Lys Leu Glu Ile Lys Arg
785 790 <210> SEQ ID NO 181 <211> LENGTH: 790
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1(P238S/P331S)-STD2-2e12HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (129)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(537) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (538)..(658) <223> OTHER INFORMATION:
VH2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (659)..(678) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (679)..(790) <223> OTHER INFORMATION:
VL2 <400> SEQUENCE: 181 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Ser Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440
445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys
Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Tyr
Gly Gly Gly Gly Ser Gly Gly Gly Gly 515 520 525 Ser Gly Gly Gly Gly
Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser 530 535 540 Gly Pro Gly
Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 545 550 555 560
Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 565
570 575 Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp
Gly 580 585 590 Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser
Ile Thr Lys 595 600 605 Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met
Asn Ser Leu Gln Thr 610 615 620 Asp Asp Thr Ala Arg Tyr Tyr Cys Ala
Arg Asp Gly Tyr Ser Asn Phe 625 630 635 640 His Tyr Tyr Val Met Asp
Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 645 650 655 Ser Ser Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 660 665 670 Ser Gly
Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 675 680 685
Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser 690
695 700 Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln
Gln 705 710 715 720 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala
Ala Ser Asn Val 725 730 735 Glu Ser Gly Val Pro Ala Arg Phe Ser Gly
Ser Gly Ser Gly Thr Asp 740 745 750 Phe Ser Leu Asn Ile His Pro Val
Glu Glu Asp Asp Ile Ala Met Tyr 755 760 765 Phe Cys Gln Gln Ser Arg
Lys Val Pro Trp Thr Phe Gly Gly Gly Thr 770 775 780 Lys Leu Glu Ile
Lys Arg 785 790 <210> SEQ ID NO 182 <211> LENGTH: 2283
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H1-2e12HL (DNA) <400> SEQUENCE: 182 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attctcaggt gcagctgaag gagtcaggac ctggcctggt ggcgccctca
1560 cagagcctgt ccatcacatg caccgtctca gggttctcat taaccggcta
tggtgtaaac 1620 tgggttcgcc agcctccagg aaagggtctg gagtggctgg
gaatgatatg gggtgatgga 1680 agcacagact ataattcagc tctcaaatcc
agactatcga tcaccaagga caactccaag 1740 agccaagttt tcttaaaaat
gaacagtctg caaactgatg acacagccag atactactgt 1800
gctcgagatg gttatagtaa ctttcattac tatgttatgg actactgggg tcaaggaacc
1860 tcagtcaccg tctcctctgg gggtggaggc tctggtggcg gtggatccgg
cggaggtggg 1920 tcgggtggcg gcggatctga cattgtgctc acccaatctc
cagcttcttt ggctgtgtct 1980 ctaggtcaga gagccaccat ctcctgcaga
gccagtgaaa gtgttgaata ttatgtcaca 2040 agtttaatgc agtggtacca
acagaaacca ggacagccac ccaaactcct catctctgct 2100 gctagcaacg
tagaatctgg ggtccctgcc aggtttagtg gcagtgggtc tgggacagac 2160
tttagcctca acatccatcc tgtggaggag gatgatattg caatgtattt ctgtcagcaa
2220 agtaggaagg ttccatggac gttcggtgga ggcaccaagc tggaaatcaa
acgttaatct 2280 aga 2283 <210> SEQ ID NO 183 <211>
LENGTH: 754 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H1-2e12HL (w/2e12 leader) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(281) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(501) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (502)..(622) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (623)..(642) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (643)..(754) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 183 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Asn Ser Gln Val Gln Leu Lys Glu
Ser Gly Pro Gly Leu 500 505 510 Val Ala Pro Ser Gln Ser Leu Ser Ile
Thr Cys Thr Val Ser Gly Phe 515 520 525 Ser Leu Thr Gly Tyr Gly Val
Asn Trp Val Arg Gln Pro Pro Gly Lys 530 535 540 Gly Leu Glu Trp Leu
Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr 545 550 555 560 Asn Ser
Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys 565 570 575
Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 580
585 590 Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr
Val 595 600 605 Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser
Ser Gly Gly 610 615 620 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly 625 630 635 640 Gly Ser Asp Ile Val Leu Thr Gln
Ser Pro Ala Ser Leu Ala Val Ser 645 650 655 Leu Gly Gln Arg Ala Thr
Ile Ser Cys Arg Ala Ser Glu Ser Val Glu 660 665 670 Tyr Tyr Val Thr
Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln 675 680 685 Pro Pro
Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val 690 695 700
Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn 705
710 715 720 Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys
Gln Gln 725 730 735 Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr
Lys Leu Glu Ile 740 745 750 Lys Arg <210> SEQ ID NO 184
<211> LENGTH: 2301 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H2-2e12HL (DNA) <400> SEQUENCE:
184 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat
cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc tcccagtctc
cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat gacttgcagg
gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga agccaggatc
ctcccccaaa ccctggattt atgccccatc caacctggct 240 tctggagtcc
ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300
agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca
360 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc
gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct tatctacagc
agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa gatgtcctgc
aaggcttctg gctacacatt taccagttac 540 aatatgcact gggtaaagca
gacacctaga cagggcctgg aatggattgg agctatttat 600 ccaggaaatg
gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660
gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg
720 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt
cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat caggagccca
aatcttctga caaaactcac 840 acatccccac cgagcccagc acctgaactc
ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca aggacaccct
catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 gacgtgagcc
acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc
1080
gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc
1140 aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg
gcagccccga 1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc
tgaccaagaa ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc
agcgacatcg ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta
caagaccacg cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca
gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440
tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct
1500 ccgggtaagg gtggcggtgg ctcggggaat tctcaggtgc agctgaagga
gtcaggacct 1560 ggcctggtgg cgccctcaca gagcctgtcc atcacatgca
ccgtctcagg gttctcatta 1620 accggctatg gtgtaaactg ggttcgccag
cctccaggaa agggtctgga gtggctggga 1680 atgatatggg gtgatggaag
cacagactat aattcagctc tcaaatccag actatcgatc 1740 accaaggaca
actccaagag ccaagttttc ttaaaaatga acagtctgca aactgatgac 1800
acagccagat actactgtgc tcgagatggt tatagtaact ttcattacta tgttatggac
1860 tactggggtc aaggaacctc agtcaccgtc tcctctgggg gtggaggctc
tggtggcggt 1920 ggatccggcg gaggtgggtc gggtggcggc ggatctgaca
ttgtgctcac ccaatctcca 1980 gcttctttgg ctgtgtctct aggtcagaga
gccaccatct cctgcagagc cagtgaaagt 2040 gttgaatatt atgtcacaag
tttaatgcag tggtaccaac agaaaccagg acagccaccc 2100 aaactcctca
tctctgctgc tagcaacgta gaatctgggg tccctgccag gtttagtggc 2160
agtgggtctg ggacagactt tagcctcaac atccatcctg tggaggagga tgatattgca
2220 atgtatttct gtcagcaaag taggaaggtt ccatggacgt tcggtggagg
caccaagctg 2280 gaaatcaaac gttaatctag a 2301 <210> SEQ ID NO
185 <211> LENGTH: 760 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H2-2e12HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(127) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(128)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(507) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (508)..(628) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (629)..(648) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (649)..(760) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 185 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Gly Gly Gly Gly Ser Gly Asn Ser
Gln Val Gln Leu Lys 500 505 510 Glu Ser Gly Pro Gly Leu Val Ala Pro
Ser Gln Ser Leu Ser Ile Thr 515 520 525 Cys Thr Val Ser Gly Phe Ser
Leu Thr Gly Tyr Gly Val Asn Trp Val 530 535 540 Arg Gln Pro Pro Gly
Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly 545 550 555 560 Asp Gly
Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile 565 570 575
Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu 580
585 590 Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr
Ser 595 600 605 Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly
Thr Ser Val 610 615 620 Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly
Gly Gly Ser Gly Gly 625 630 635 640 Gly Gly Ser Gly Gly Gly Gly Ser
Asp Ile Val Leu Thr Gln Ser Pro 645 650 655 Ala Ser Leu Ala Val Ser
Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg 660 665 670 Ala Ser Glu Ser
Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr 675 680 685 Gln Gln
Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser 690 695 700
Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 705
710 715 720 Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp
Ile Ala 725 730 735 Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp
Thr Phe Gly Gly 740 745 750 Gly Thr Lys Leu Glu Ile Lys Arg 755 760
<210> SEQ ID NO 186 <211> LENGTH: 2307 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2H7sssIgG1-H3-2e12HL (DNA)
<400> SEQUENCE: 186 aagcttgccg ccatggattt tcaagtgcag
attttcagct tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca
aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga
aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180
taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct
240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgagcccagc
acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg cggtggctct
gggaattctc aggtgcagct gaaggagtca 1560 ggacctggcc tggtggcgcc
ctcacagagc ctgtccatca catgcaccgt ctcagggttc 1620 tcattaaccg
gctatggtgt aaactgggtt cgccagcctc caggaaaggg tctggagtgg 1680
ctgggaatga tatggggtga tggaagcaca gactataatt cagctctcaa atccagacta
1740 tcgatcacca aggacaactc caagagccaa gttttcttaa aaatgaacag
tctgcaaact 1800 gatgacacag ccagatacta ctgtgctcga gatggttata
gtaactttca ttactatgtt 1860 atggactact ggggtcaagg aacctcagtc
accgtctcct ctgggggtgg aggctctggt 1920 ggcggtggat ccggcggagg
tgggtcgggt ggcggcggat ctgacattgt gctcacccaa 1980 tctccagctt
ctttggctgt gtctctaggt cagagagcca ccatctcctg cagagccagt 2040
gaaagtgttg aatattatgt cacaagttta atgcagtggt accaacagaa accaggacag
2100 ccacccaaac tcctcatctc tgctgctagc aacgtagaat ctggggtccc
tgccaggttt 2160 agtggcagtg ggtctgggac agactttagc ctcaacatcc
atcctgtgga ggaggatgat 2220 attgcaatgt atttctgtca gcaaagtagg
aaggttccat ggacgttcgg tggaggcacc 2280 aagctggaaa tcaaacgtta atctaga
2307 <210> SEQ ID NO 187 <211> LENGTH: 762 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2H7sssIgG1-H3-2e12HL
(w/2e12 leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(282) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(509) <223> OTHER
INFORMATION: EFD-BD2 Linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (510)..(630)
<223> OTHER INFORMATION: VH2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (631)..(650)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(651)..(762) <223> OTHER INFORMATION: VL2 <400>
SEQUENCE: 187 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser Gln
Val Gln 500 505 510 Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser
Gln Ser Leu Ser 515 520 525 Ile Thr Cys Thr Val Ser Gly Phe Ser Leu
Thr Gly Tyr Gly Val Asn 530 535 540 Trp Val Arg Gln Pro Pro Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile 545 550 555 560 Trp Gly Asp Gly Ser
Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu 565 570 575 Ser Ile Thr
Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 580 585 590 Ser
Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly 595 600
605 Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr
610 615 620 Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp
Ile Val Leu Thr Gln 645 650 655 Ser Pro Ala Ser Leu Ala Val Ser Leu
Gly Gln Arg Ala Thr Ile Ser 660 665 670 Cys Arg Ala Ser Glu Ser Val
Glu Tyr Tyr Val Thr Ser Leu Met Gln 675 680 685 Trp Tyr Gln Gln Lys
Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala 690 695 700 Ala Ser Asn
Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly 705 710 715
720
Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp 725
730 735 Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr
Phe 740 745 750 Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 755 760
<210> SEQ ID NO 188 <211> LENGTH: 2316 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2H7sssIgG1-H4-2e12HL (DNA)
<400> SEQUENCE: 188 aagcttgccg ccatggattt tcaagtgcag
attttcagct tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca
aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga
aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180
taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct
240 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc
tctcacaatc 300 agcagagtgg aggctgaaga tgctgccact tattactgcc
agcagtggag ttttaaccca 360 cccacgttcg gtgctgggac caagctggag
ctgaaagatg gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag
ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg
cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540
aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat
600 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac
actgactgta 660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc
tgacatctga agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat
agtaactctt actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac
cgtctctgat caggagccca aatcttctga caaaactcac 840 acatccccac
cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900
ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg
960 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
cgtggaggtg 1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca
gcacgtaccg tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg
aatggcaagg agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc
catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg
tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260
ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat
1320 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
cggctccttc 1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc
agcaggggaa cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac
cactacacgc agaagagcct ctccctgtct 1500 ccgggtaagg gtggcggtgg
ctcgggcggt ggtggatctg ggaattctca ggtgcagctg 1560 aaggagtcag
gacctggcct ggtggcgccc tcacagagcc tgtccatcac atgcaccgtc 1620
tcagggttct cattaaccgg ctatggtgta aactgggttc gccagcctcc aggaaagggt
1680 ctggagtggc tgggaatgat atggggtgat ggaagcacag actataattc
agctctcaaa 1740 tccagactat cgatcaccaa ggacaactcc aagagccaag
ttttcttaaa aatgaacagt 1800 ctgcaaactg atgacacagc cagatactac
tgtgctcgag atggttatag taactttcat 1860 tactatgtta tggactactg
gggtcaagga acctcagtca ccgtctcctc tgggggtgga 1920 ggctctggtg
gcggtggatc cggcggaggt gggtcgggtg gcggcggatc tgacattgtg 1980
ctcacccaat ctccagcttc tttggctgtg tctctaggtc agagagccac catctcctgc
2040 agagccagtg aaagtgttga atattatgtc acaagtttaa tgcagtggta
ccaacagaaa 2100 ccaggacagc cacccaaact cctcatctct gctgctagca
acgtagaatc tggggtccct 2160 gccaggttta gtggcagtgg gtctgggaca
gactttagcc tcaacatcca tcctgtggag 2220 gaggatgata ttgcaatgta
tttctgtcag caaagtagga aggttccatg gacgttcggt 2280 ggaggcacca
agctggaaat caaacgttaa tctaga 2316 <210> SEQ ID NO 189
<211> LENGTH: 765 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: 2H7sssIgG1-H4-2e12HL (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(512) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (513)..(633) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (634)..(653) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (654)..(765) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 189 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Asn Ser 500 505 510 Gln Val Gln Leu Lys Glu Ser Gly Pro
Gly Leu Val Ala Pro Ser Gln 515 520 525 Ser Leu Ser Ile Thr Cys Thr
Val Ser Gly Phe Ser Leu Thr Gly Tyr 530 535 540 Gly Val Asn Trp Val
Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 545 550 555 560 Gly Met
Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys 565 570
575
Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 580
585 590 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys
Ala 595 600 605 Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp
Tyr Trp Gly 610 615 620 Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly
Gly Gly Ser Gly Gly 625 630 635 640 Gly Gly Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Asp Ile Val 645 650 655 Leu Thr Gln Ser Pro Ala
Ser Leu Ala Val Ser Leu Gly Gln Arg Ala 660 665 670 Thr Ile Ser Cys
Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser 675 680 685 Leu Met
Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 690 695 700
Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser 705
710 715 720 Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro
Val Glu 725 730 735 Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser
Arg Lys Val Pro 740 745 750 Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu
Ile Lys Arg 755 760 765 <210> SEQ ID NO 190 <211>
LENGTH: 2322 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H5-2e12HL (DNA) <400> SEQUENCE: 190 aagcttgccg
ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60
gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct
120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
catgcactgg 180 taccagcaga agccaggatc ctcccccaaa ccctggattt
atgccccatc caacctggct 240 tctggagtcc ctgctcgctt cagtggcagt
gggtctggga cctcttactc tctcacaatc 300 agcagagtgg aggctgaaga
tgctgccact tattactgcc agcagtggag ttttaaccca 360 cccacgttcg
gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420
ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg
480 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt
taccagttac 540 aatatgcact gggtaaagca gacacctaga cagggcctgg
aatggattgg agctatttat 600 ccaggaaatg gtgatacttc ctacaatcag
aagttcaagg gcaaggccac actgactgta 660 gacaaatcct ccagcacagc
ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 gtctatttct
gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780
ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac
840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt
cctcttcccc 900 ccaaaaccca aggacaccct catgatctcc cggacccctg
aggtcacatg cgtggtggtg 960 gacgtgagcc acgaagaccc tgaggtcaag
ttcaactggt acgtggacgg cgtggaggtg 1020 cataatgcca agacaaagcc
gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 gtcctcaccg
tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140
aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga
1200 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
ccaggtcagc 1260 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
ccgtggagtg ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg
cctcccgtgc tggactccga cggctccttc 1380 ttcctctaca gcaagctcac
cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440 tgctccgtga
tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500
ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctgggaa ttctcaggtg
1560 cagctgaagg agtcaggacc tggcctggtg gcgccctcac agagcctgtc
catcacatgc 1620 accgtctcag ggttctcatt aaccggctat ggtgtaaact
gggttcgcca gcctccagga 1680 aagggtctgg agtggctggg aatgatatgg
ggtgatggaa gcacagacta taattcagct 1740 ctcaaatcca gactatcgat
caccaaggac aactccaaga gccaagtttt cttaaaaatg 1800 aacagtctgc
aaactgatga cacagccaga tactactgtg ctcgagatgg ttatagtaac 1860
tttcattact atgttatgga ctactggggt caaggaacct cagtcaccgt ctcctctggg
1920 ggtggaggct ctggtggcgg tggatccggc ggaggtgggt cgggtggcgg
cggatctgac 1980 attgtgctca cccaatctcc agcttctttg gctgtgtctc
taggtcagag agccaccatc 2040 tcctgcagag ccagtgaaag tgttgaatat
tatgtcacaa gtttaatgca gtggtaccaa 2100 cagaaaccag gacagccacc
caaactcctc atctctgctg ctagcaacgt agaatctggg 2160 gtccctgcca
ggtttagtgg cagtgggtct gggacagact ttagcctcaa catccatcct 2220
gtggaggagg atgatattgc aatgtatttc tgtcagcaaa gtaggaaggt tccatggacg
2280 ttcggtggag gcaccaagct ggaaatcaaa cgttaatcta ga 2322
<210> SEQ ID NO 191 <211> LENGTH: 767 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-H5-2e12HL (w/2e12 leader)
(AA) <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (1)..(22) <223> OTHER INFORMATION:
Leader <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (23)..(128) <223> OTHER INFORMATION: VL
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (129)..(144) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (145)..(265) <223> OTHER INFORMATION: VH
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (268)..(282) <223> OTHER INFORMATION: Hinge
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (500)..(514) <223> OTHER INFORMATION: EFD-BD2
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (515)..(635) <223> OTHER INFORMATION:
VH2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (636)..(655) <223> OTHER INFORMATION:
Linker2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (656)..(767) <223> OTHER INFORMATION:
VL2 <400> SEQUENCE: 191 Met Asp Phe Gln Val Gln Ile Phe Ser
Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln
Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro
Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val
Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro
Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70
75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr
Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr
Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser
Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met
His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190
Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195
200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr
Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val
Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser
Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315
320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys
Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435
440 445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
Val 450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
Ser Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln
Lys Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510 Asn Ser Gln Val Gln Leu
Lys Glu Ser Gly Pro Gly Leu Val Ala Pro 515 520 525 Ser Gln Ser Leu
Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 530 535 540 Gly Tyr
Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 545 550 555
560 Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala
565 570 575 Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser
Gln Val 580 585 590 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr
Ala Arg Tyr Tyr 595 600 605 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His
Tyr Tyr Val Met Asp Tyr 610 615 620 Trp Gly Gln Gly Thr Ser Val Thr
Val Ser Ser Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 645 650 655 Ile Val Leu
Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 660 665 670 Arg
Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 675 680
685 Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys
690 695 700 Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro
Ala Arg 705 710 715 720 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser
Leu Asn Ile His Pro 725 730 735 Val Glu Glu Asp Asp Ile Ala Met Tyr
Phe Cys Gln Gln Ser Arg Lys 740 745 750 Val Pro Trp Thr Phe Gly Gly
Gly Thr Lys Leu Glu Ile Lys Arg 755 760 765 <210> SEQ ID NO
192 <211> LENGTH: 2331 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H6-2e12HL (DNA) <400> SEQUENCE:
192 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat
cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc tcccagtctc
cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat gacttgcagg
gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga agccaggatc
ctcccccaaa ccctggattt atgccccatc caacctggct 240 tctggagtcc
ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300
agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca
360 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc
gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct tatctacagc
agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa gatgtcctgc
aaggcttctg gctacacatt taccagttac 540 aatatgcact gggtaaagca
gacacctaga cagggcctgg aatggattgg agctatttat 600 ccaggaaatg
gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660
gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg
720 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt
cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat caggagccca
aatcttctga caaaactcac 840 acatccccac cgagcccagc acctgaactc
ctggggggac cgtcagtctt cctcttcccc 900 ccaaaaccca aggacaccct
catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 gacgtgagcc
acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc
1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa acaatctcca
aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct gcccccatcc
cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc tggtcaaagg
cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320 gggcagccgg
agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380
ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca
1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct
ctccctgtct 1500 ccgggtaagg gtggcggtgg ctcgggcggt ggtggatctg
ggggaggagg cagcgggaat 1560 tctcaggtgc agctgaagga gtcaggacct
ggcctggtgg cgccctcaca gagcctgtcc 1620 atcacatgca ccgtctcagg
gttctcatta accggctatg gtgtaaactg ggttcgccag 1680 cctccaggaa
agggtctgga gtggctggga atgatatggg gtgatggaag cacagactat 1740
aattcagctc tcaaatccag actatcgatc accaaggaca actccaagag ccaagttttc
1800 ttaaaaatga acagtctgca aactgatgac acagccagat actactgtgc
tcgagatggt 1860 tatagtaact ttcattacta tgttatggac tactggggtc
aaggaacctc agtcaccgtc 1920 tcctctgggg gtggaggctc tggtggcggt
ggatccggcg gaggtgggtc gggtggcggc 1980 ggatctgaca ttgtgctcac
ccaatctcca gcttctttgg ctgtgtctct aggtcagaga 2040 gccaccatct
cctgcagagc cagtgaaagt gttgaatatt atgtcacaag tttaatgcag 2100
tggtaccaac agaaaccagg acagccaccc aaactcctca tctctgctgc tagcaacgta
2160 gaatctgggg tccctgccag gtttagtggc agtgggtctg ggacagactt
tagcctcaac 2220 atccatcctg tggaggagga tgatattgca atgtatttct
gtcagcaaag taggaaggtt 2280 ccatggacgt tcggtggagg caccaagctg
gaaatcaaac gttaatctag a 2331 <210> SEQ ID NO 193 <211>
LENGTH: 770 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
2H7sssIgG1-H6-2e12HL (w/2e12 leader) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(517) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (518)..(638) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (639)..(658) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (659)..(770) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 193 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290
295 300 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
His 305 310 315 320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp
Gly Val Glu Val 325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu
Gln Tyr Asn Ser Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val
Leu His Gln Asp Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys
Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410
415 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
420 425 430 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
Pro Pro 435 440 445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
Lys Leu Thr Val 450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
Phe Ser Cys Ser Val Met 465 470 475 480 His Glu Ala Leu His Asn His
Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 500 505 510 Gly Ser Gly
Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu 515 520 525 Val
Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe 530 535
540 Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys
545 550 555 560 Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser
Thr Asp Tyr 565 570 575 Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys Asp Asn Ser Lys 580 585 590 Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr Asp Asp Thr Ala 595 600 605 Arg Tyr Tyr Cys Ala Arg Asp
Gly Tyr Ser Asn Phe His Tyr Tyr Val 610 615 620 Met Asp Tyr Trp Gly
Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly 625 630 635 640 Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 645 650 655
Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser 660
665 670 Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val
Glu 675 680 685 Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys
Pro Gly Gln 690 695 700 Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn
Val Glu Ser Gly Val 705 710 715 720 Pro Ala Arg Phe Ser Gly Ser Gly
Ser Gly Thr Asp Phe Ser Leu Asn 725 730 735 Ile His Pro Val Glu Glu
Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln 740 745 750 Ser Arg Lys Val
Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 755 760 765 Lys Arg
770 <210> SEQ ID NO 194 <211> LENGTH: 2301 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: 2H7sscIgG1-H7-2e12HL (DNA)
<400> SEQUENCE: 194 aagcttgccg ccatggattt tcaagtgcag
attttcagct tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca
aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga
aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180
taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct
240 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc
tctcacaatc 300 agcagagtgg aggctgaaga tgctgccact tattactgcc
agcagtggag ttttaaccca 360 cccacgttcg gtgctgggac caagctggag
ctgaaagatg gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag
ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg
cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540
aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat
600 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac
actgactgta 660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc
tgacatctga agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat
agtaactctt actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac
cgtctctgat caggagccca aatcttctga caaaactcac 840 acatccccac
cgtgcccagc acctgaactc ctggggggat cgtcagtctt cctcttcccc 900
ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg
960 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
cgtggaggtg 1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca
gcacgtaccg tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg
aatggcaagg agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc
catcgagaaa accatctcca aagccaaagg gcagccccga 1200 gaaccacagg
tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260
ctgacctgcc tggtcaaagg cttctatcca agcgacatcg ccgtggagtg ggagagcaat
1320 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
cggctccttc 1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc
agcaggggaa cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac
cactacacgc agaagagcct ctccctgtct 1500 ccgggtaagg ggtgtccacc
ttgtccgaat tctcaggtgc agctgaagga gtcagggcct 1560 ggcagcgtgg
cgccctcaca gagcctgtcc atcacatgca ccgtctcagg gttctcatta 1620
accggctatg gtgtaaactg ggttcgccag cctccaggaa agggtctgga gtggctggga
1680 atgatatggg gtgatggaag cacagactat aattcagctc tcaaatccag
actatcgatc 1740 accaaggaca actccaagag ccaagttttc ttaaaaatga
acagtctgca aactgatgac 1800 acagccagat actactgtgc tcgagatggt
tatagtaact ttcattacta tgttatggac 1860 tactggggtc aaggaacctc
agtcaccgtc tcctctgggg gtggaggctc tggtggcggt 1920 ggatccggcg
gaggtgggtc gggtggcggc ggatctgaca ttgtgctcac ccaatctcca 1980
gcttctttgg ctgtgtctct aggtcagaga gccaccatct cctgcagagc cagtgaaagt
2040 gttgaatatt atgtcacaag tttaatgcag tggtaccaac agaaaccagg
acagccaccc 2100 aagctcctca tctctgctgc tagcaacgta gaatctgggg
tccctgccag gtttagtggc 2160 agtgggtctg ggacagactt tagcctcaac
atccatcctg tggaggagga tgatattgca 2220 atgtatttct gtcagcaaag
taggaaggtt ccatggacgt tcggtggagg caccaagctg 2280 gaaatcaaac
gttaatctag a 2301 <210> SEQ ID NO 195 <211> LENGTH: 760
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
2H7sscIgG1-H7-2e12HL (w/2e12 linker ) (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(23)..(128) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(507) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (508)..(628) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (629)..(648) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (649)..(760) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 195 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125
Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130
135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly
Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr
Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln Thr Pro Arg
Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro Gly Asn Gly
Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys Ala Thr Leu
Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met Gln Leu Ser
Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 240 Ala
Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250
255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser
260 265 270 Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu
Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val Thr Cys Val
Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu Val Lys Phe
Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn Ala Lys Thr
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350 Arg Val Val
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365 Lys
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375
380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val
385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln Pro Glu Asn
Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser Asp Gly Ser
Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys Ser Arg Trp
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 480 His Glu
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 495
Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser Gln Val Gln Leu Lys 500
505 510 Glu Ser Gly Pro Gly Ser Val Ala Pro Ser Gln Ser Leu Ser Ile
Thr 515 520 525 Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val
Asn Trp Val 530 535 540 Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu
Gly Met Ile Trp Gly 545 550 555 560 Asp Gly Ser Thr Asp Tyr Asn Ser
Ala Leu Lys Ser Arg Leu Ser Ile 565 570 575 Thr Lys Asp Asn Ser Lys
Ser Gln Val Phe Leu Lys Met Asn Ser Leu 580 585 590 Gln Thr Asp Asp
Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser 595 600 605 Asn Phe
His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 610 615 620
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 625
630 635 640 Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln
Ser Pro 645 650 655 Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr
Ile Ser Cys Arg 660 665 670 Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr
Ser Leu Met Gln Trp Tyr 675 680 685 Gln Gln Lys Pro Gly Gln Pro Pro
Lys Leu Leu Ile Ser Ala Ala Ser 690 695 700 Asn Val Glu Ser Gly Val
Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 705 710 715 720 Thr Asp Phe
Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala 725 730 735 Met
Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly 740 745
750 Gly Thr Lys Leu Glu Ile Lys Arg 755 760 <210> SEQ ID NO
196 <211> LENGTH: 2283 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H7-G194 HL (DNA) <400>
SEQUENCE: 196 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgtgcccagc
acctgaactc ctggggggat cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
accatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatcca agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaagg ggtgtccacc ttgtccgaat
tctgaggtcc agctgcaaca gtctggacct 1560 gaactggtga agcctggagc
ttcaatgaag atttcctgca aggcctctgg ttactcattc 1620 actggctaca
tcgtgaactg gctgaagcag agccatggaa agaaccttga gtggattgga 1680
cttattaatc catacaaagg tcttactacc tacaaccaga aattcaaggg caaggccaca
1740 ttaactgtag acaagtcatc cagcacagcc tacatggagc tcctcagtct
gacatctgaa 1800 gactctgcag tctattactg tgcaagatct gggtactatg
gtgactcgga ctggtacttc 1860 gatgtctggg gcgcagggac cacggtcacc
gtctcctctg gtggcggtgg ctcgggcggt 1920 ggtggatctg gaggaggtgg
gagcgctagc gacatccaga tgacacagac tacatcctcc 1980 ctgtctgcct
ctctgggaga cagagtcacc atcagttgca gggcaagtca ggacattcgc 2040
aattatttaa actggtatca gcagaaacca gatggaactg ttaaactcct gatctactac
2100 acatcaagat tacactcagg agtcccatca aggttcagtg gcagtgggtc
tggaacagat 2160 tattctctca ccattgccaa cctgcaacca gaagatattg
ccacttactt ttgccaacag 2220 ggtaatacgc ttccgtggac gttcggtgga
ggcaccaaac tggtaaccaa acggtaatct 2280 aga 2283 <210> SEQ ID
NO 197 <211> LENGTH: 754 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H7-G194 HL (w/2e12 leader) (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(22) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (23)..(128) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(129)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(265) <223> OTHER INFORMATION: VH1 <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(282) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(500)..(507) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (508)..(629) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (630)..(646) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (647)..(754) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 197 Met Asp Phe Gln Val Gln Ile Phe Ser Phe
Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile
Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly
Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser
Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys
Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85
90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln
Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu
Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala
Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp
Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala
Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210
215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe
Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp
Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His Thr Ser Pro Pro
Cys Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser Ser Val Phe Leu
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330
335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455
460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
Ser Leu Ser 485 490 495 Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser
Glu Val Gln Leu Gln 500 505 510 Gln Ser Gly Pro Glu Leu Val Lys Pro
Gly Ala Ser Met Lys Ile Ser 515 520 525 Cys Lys Ala Ser Gly Tyr Ser
Phe Thr Gly Tyr Ile Val Asn Trp Leu 530 535 540 Lys Gln Ser His Gly
Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro 545 550 555 560 Tyr Lys
Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr 565 570 575
Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser 580
585 590 Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Gly
Tyr 595 600 605 Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala
Gly Thr Thr 610 615 620 Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 625 630 635 640 Gly Gly Gly Ser Ala Ser Asp Ile
Gln Met Thr Gln Thr Thr Ser Ser 645 650 655 Leu Ser Ala Ser Leu Gly
Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 660 665 670 Gln Asp Ile Arg
Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 675 680 685 Thr Val
Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu His Ser Gly Val 690 695 700
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 705
710 715 720 Ile Ala Asn Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys
Gln Gln 725 730 735 Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly Gly Thr
Lys Leu Val Thr 740 745 750 Lys Arg <210> SEQ ID NO 198
<211> LENGTH: 2298 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: 2H7sssIgG1-H7-G281 HL (DNA) <400>
SEQUENCE: 198 aagcttgccg ccatggattt tcaagtgcag attttcagct
tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca aattgttctc
tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga aggtcacaat
gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 taccagcaga
agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240
tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc
300 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag
ttttaaccca 360 cccacgttcg gtgctgggac caagctggag ctgaaagatg
gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag ctctcaggct
tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg cctcagtgaa
gatgtcctgc aaggcttctg gctacacatt taccagttac 540 aatatgcact
gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600
ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta
660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga
agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat agtaactctt
actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac cgtctctgat
caggagccca aatcttctga caaaactcac 840 acatccccac cgtgcccagc
acctgaactc ctggggggat cgtcagtctt cctcttcccc 900 ccaaaaccca
aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg aatggcaagg
agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc catcgagaaa
accatctcca aagccaaagg gcagccccga 1200 gaaccacagg tgtacaccct
gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 ctgacctgcc
tggtcaaagg cttctatcca agcgacatcg ccgtggagtg ggagagcaat 1320
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac cactacacgc
agaagagcct ctccctgtct 1500 ccgggtaagg ggtgtccacc ttgtccgaat
tctgaggtcc agctgcaaca gtctggacct 1560 gaactggtga agcctggagc
ttcaatgaag atttcctgca aggcctctgg ttactcattc 1620 actggctaca
tcgtgaactg gctgaagcag agccatggaa agaaccttga gtggattgga 1680
cttattaatc catacaaagg tcttactacc tacaaccaga aattcaaggg caaggccaca
1740 ttaactgtag acaagtcatc cagcacagcc tacatggagc tcctcagtct
gacatctgaa 1800 gactctgcag tctattactg tgcaagatct gggtactatg
gtgactcgga ctggtacttc 1860 gatgtctggg gcgcagggac cacggtcacc
gtctcctctg gtggcggtgg ctcgggcggt 1920 ggtggatctg gaggaggtgg
gagcggggga ggtggcagtg ctagcgacat ccagatgaca 1980 cagactacat
cctccctgtc tgcctctctg ggagacagag tcaccatcag ttgcagggca 2040
agtcaggaca ttcgcaatta tttaaactgg tatcagcaga aaccagatgg aactgttaaa
2100 ctcctgatct actacacatc aagattacac tcaggagtcc catcaaggtt
cagtggcagt 2160 gggtctggaa cagattattc tctcaccatt gccaacctgc
aaccagaaga tattgccact 2220 tacttttgcc aacagggtaa tacgcttccg
tggacgttcg gtggaggcac caaactggta 2280 accaaacggt aatctaga 2298
<210> SEQ ID NO 199 <211> LENGTH: 759 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-H7-G281 HL (w/2e12
leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL1 <220> FEATURE: <221> NAME/KEY:
misc_feature
<222> LOCATION: (129)..(144) <223> OTHER INFORMATION:
Linker <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (145)..(265) <223> OTHER INFORMATION:
VH1 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (268)..(282) <223> OTHER INFORMATION:
Hinge <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (500)..(507) <223> OTHER INFORMATION:
EFD-BD2 Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (508)..(629) <223> OTHER
INFORMATION: VH2 <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (630)..(651) <223> OTHER
INFORMATION: Linker2 <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (652)..(759) <223> OTHER
INFORMATION: VL2 <400> SEQUENCE: 199 Met Asp Phe Gln Val Gln
Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met Ser
Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 Leu Ser
Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45
Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50
55 60 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val
Pro 65 70 75 80 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser
Leu Thr Ile 85 90 95 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr
Tyr Cys Gln Gln Trp 100 105 110 Ser Phe Asn Pro Pro Thr Phe Gly Ala
Gly Thr Lys Leu Glu Leu Lys 115 120 125 Asp Gly Gly Gly Ser Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140 Gln Ala Tyr Leu Gln
Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 Ser Val
Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175
Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180
185 190 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys
Phe 195 200 205 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser
Thr Ala Tyr 210 215 220 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser
Ala Val Tyr Phe Cys 225 230 235 240 Ala Arg Val Val Tyr Tyr Ser Asn
Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 255 Gly Thr Gly Thr Thr Val
Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 Asp Lys Thr His
Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 275 280 285 Gly Ser
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305
310 315 320 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
Glu Val 325 330 335 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr
Asn Ser Thr Tyr 340 345 350 Arg Val Val Ser Val Leu Thr Val Leu His
Gln Asp Trp Leu Asn Gly 355 360 365 Lys Glu Tyr Lys Cys Lys Val Ser
Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 Glu Lys Thr Ile Ser Lys
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 Tyr Thr Leu
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 Leu
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425
430 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro
435 440 445 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
Thr Val 450 455 460 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser
Cys Ser Val Met 465 470 475 480 His Glu Ala Leu His Asn His Tyr Thr
Gln Lys Ser Leu Ser Leu Ser 485 490 495 Pro Gly Lys Gly Cys Pro Pro
Cys Pro Asn Ser Glu Val Gln Leu Gln 500 505 510 Gln Ser Gly Pro Glu
Leu Val Lys Pro Gly Ala Ser Met Lys Ile Ser 515 520 525 Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly Tyr Ile Val Asn Trp Leu 530 535 540 Lys
Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro 545 550
555 560 Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys Ala
Thr 565 570 575 Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu
Leu Leu Ser 580 585 590 Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys
Ala Arg Ser Gly Tyr 595 600 605 Tyr Gly Asp Ser Asp Trp Tyr Phe Asp
Val Trp Gly Ala Gly Thr Thr 610 615 620 Val Thr Val Ser Ser Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Gly 625 630 635 640 Gly Gly Gly Ser
Gly Gly Gly Gly Ser Ala Ser Asp Ile Gln Met Thr 645 650 655 Gln Thr
Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 660 665 670
Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln 675
680 685 Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser
Arg 690 695 700 Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly
Ser Gly Thr 705 710 715 720 Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln
Pro Glu Asp Ile Ala Thr 725 730 735 Tyr Phe Cys Gln Gln Gly Asn Thr
Leu Pro Trp Thr Phe Gly Gly Gly 740 745 750 Thr Lys Leu Val Thr Lys
Arg 755 <210> SEQ ID NO 200 <211> LENGTH: 1533
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: 2e12-sss-IgG1
HL SMIP (DNA) <400> SEQUENCE: 200 atggattttc aagtgcagat
tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60 agaggagtcc
aggtgcagct gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 120
ctgtccatca catgcaccgt ctcagggttc tcattaaccg gctatggtgt aaactgggtt
180 cgccagcctc caggaaaggg tctagagtgg ctgggaatga tatggggtga
tggaagcaca 240 gactataatt cagctctcaa atccagacta tcgatcacca
aggacaactc caagagccaa 300 gttttcttaa aaatgaacag tctgcaaact
gatgacacag ccagatacta ctgtgctcga 360 gatggttata gtaactttca
ttactatgtt atggactact ggggtcaagg aacctcagtc 420 accgtctcct
ctgggggtgg aggctctggt ggcggtggat ccggcggagg tgggtcgggt 480
ggcggcggat ctgacattgt gctcacccaa tctccagctt ctttggctgt gtctctaggt
540 cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt
cacaagttta 600 atgcagtggt accaacagaa accaggacag ccacccaaac
tcctcatctc tgctgctagc 660 aacgtagaat ctggggtccc tgccaggttt
agtggcagtg ggtctgggac agactttagc 720 ctcaacatcc atcctgtgga
ggaggatgat attgcaatgt atttctgtca gcaaagtagg 780 aaggttccat
ggacgttcgg tggaggcacc aagctggaaa tcaaacgtga tcaggagccc 840
aaatcttctg acaaaactca cacatcccca ccgtccccag cacctgaact cctgggggga
900 tcgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc
ccggacccct 960 gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc
ctgaggtcaa gttcaactgg 1020 tacgtggacg gcgtggaggt gcataatgcc
aagacaaagc cgcgggagga gcagtacaac 1080 agcacgtacc gtgtggtcag
cgtcctcacc gtcctgcacc aggactggct gaatggcaag 1140 gagtacaagt
gcaaggtctc caacaaagcc ctcccagcct ccatcgagaa aacaatctcc 1200
aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag
1260 ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc
cagcgacatc 1320 gccgtggagt gggagagcaa tgggcagccg gagaacaact
acaagaccac gcctcccgtg 1380 ctggactccg acggctcctt cttcctctac
agcaagctca ccgtggacaa gagcaggtgg 1440 cagcagggga acgtcttctc
atgctccgtg atgcatgagg ctctgcacaa ccactacacg 1500 cagaagagcc
tctccctgtc tccgggtaaa tga 1533 <210> SEQ ID NO 201
<211> LENGTH: 510 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: 2e12-sss-IgG1 HL SMIP (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(22)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(24)..(144) <223> OTHER INFORMATION: VH <220>
FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(164) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(165)..(276) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(279)..(293) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 201 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Val Gln Val Gln Leu
Lys Glu Ser Gly Pro 20 25 30 Gly Leu Val Ala Pro Ser Gln Ser Leu
Ser Ile Thr Cys Thr Val Ser 35 40 45 Gly Phe Ser Leu Thr Gly Tyr
Gly Val Asn Trp Val Arg Gln Pro Pro 50 55 60 Gly Lys Gly Leu Glu
Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 65 70 75 80 Asp Tyr Asn
Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 85 90 95 Ser
Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 100 105
110 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
115 120 125 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 130 135 140 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
Gly Gly Ser Gly 145 150 155 160 Gly Gly Gly Ser Asp Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala 165 170 175 Val Ser Leu Gly Gln Arg Ala
Thr Ile Ser Cys Arg Ala Ser Glu Ser 180 185 190 Val Glu Tyr Tyr Val
Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 195 200 205 Gly Gln Pro
Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 210 215 220 Gly
Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 225 230
235 240 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe
Cys 245 250 255 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly
Thr Lys Leu 260 265 270 Glu Ile Lys Arg Asp Gln Glu Pro Lys Ser Ser
Asp Lys Thr His Thr 275 280 285 Ser Pro Pro Ser Pro Ala Pro Glu Leu
Leu Gly Gly Ser Ser Val Phe 290 295 300 Leu Phe Pro Pro Lys Pro Lys
Asp Thr Leu Met Ile Ser Arg Thr Pro 305 310 315 320 Glu Val Thr Cys
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 325 330 335 Lys Phe
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 340 345 350
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 355
360 365 Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys
Cys 370 375 380 Lys Val Ser Asn Lys Ala Leu Pro Ala Ser Ile Glu Lys
Thr Ile Ser 385 390 395 400 Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
Val Tyr Thr Leu Pro Pro 405 410 415 Ser Arg Asp Glu Leu Thr Lys Asn
Gln Val Ser Leu Thr Cys Leu Val 420 425 430 Lys Gly Phe Tyr Pro Ser
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 435 440 445 Gln Pro Glu Asn
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 450 455 460 Gly Ser
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 465 470 475
480 Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
485 490 495 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
500 505 510 <210> SEQ ID NO 202 <211> LENGTH: 1518
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: 2e12-sss-IgG1
LH SMIP (DNA) <400> SEQUENCE: 202 atggattttc aagtgcagat
tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60 agaggagtcg
acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 120
agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg
180 cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc
tgctagcaac 240 gtagaatctg gggtccctgc caggtttagt ggcagtgggt
ctgggacaga ctttagcctc 300 aacatccatc ctgtggagga ggatgatatt
gcaatgtatt tctgtcagca aagtaggaag 360 gttccatgga cgttcggtgg
aggcaccaag ctggaaatca aacggggtgg cggtggatcc 420 ggcggaggtg
ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 480
ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc
540 ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctagagtg
gctgggaatg 600 atatggggtg atggaagcac agactataat tcagctctca
aatccagact atcgatcacc 660 aaggacaact ccaagagcca agttttctta
aaaatgaaca gtctgcaaac tgatgacaca 720 gccagatact actgtgctcg
agatggttat agtaactttc attactatgt tatggactac 780 tggggtcaag
gaacctcagt caccgtctcc tctgatcagg agcccaaatc ttctgacaaa 840
actcacacat ccccaccgtc cccagcacct gaactcctgg ggggaccgtc agtcttcctc
900 ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
cacatgcgtg 960 gtggtggacg tgagccacga agaccctgag gtcaagttca
actggtacgt ggacggcgtg 1020 gaggtgcata atgccaagac aaagccgcgg
gaggagcagt acaacagcac gtaccgtgtg 1080 gtcagcgtcc tcaccgtcct
gcaccaggac tggctgaatg gcaaggagta caagtgcaag 1140 gtctccaaca
aagccctccc agcccccatc gagaaaacaa tctccaaagc caaagggcag 1200
ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag
1260 gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
ggagtgggag 1320 agcaatgggc agccggagaa caactacaag accacgcctc
ccgtgctgga ctccgacggc 1380 tccttcttcc tctacagcaa gctcaccgtg
gacaagagca ggtggcagca ggggaacgtc 1440 ttctcatgct ccgtgatgca
tgaggctctg cacaaccact acacgcagaa gagcctctcc 1500 ctgtctccgg
gtaaatga 1518 <210> SEQ ID NO 203 <211> LENGTH: 505
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: 2e12-sss-IgG1
LH SMIP (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(23) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (24)..(135) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (136)..(150) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (151)..(271) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (274)..(288) <223> OTHER
INFORMATION: Hinge <400> SEQUENCE: 203 Met Asp Phe Gln Val
Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 Val Ile Met
Ser Arg Gly Val Asp Ile Val Leu Thr Gln Ser Pro Ala 20 25 30 Ser
Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 35 40
45 Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln
50 55 60 Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala
Ser Asn 65 70 75 80 Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser
Gly Ser Gly Thr 85 90 95 Asp Phe Ser Leu Asn Ile His Pro Val Glu
Glu Asp Asp Ile Ala Met 100 105 110 Tyr Phe Cys Gln Gln Ser Arg Lys
Val Pro Trp Thr Phe Gly Gly Gly 115 120 125 Thr Lys Leu Glu Ile Lys
Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 130 135 140 Ser Gly Gly Gly
Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 145 150 155 160 Leu
Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 165 170
175 Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly
180 185 190 Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser
Thr Asp 195 200 205 Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr
Lys Asp Asn Ser 210 215 220 Lys Ser Gln Val Phe Leu Lys Met Asn Ser
Leu Gln Thr Asp Asp Thr 225 230 235 240 Ala Arg Tyr Tyr Cys Ala Arg
Asp Gly Tyr Ser Asn Phe His Tyr Tyr 245 250 255 Val Met Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp
260 265 270 Gln Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro
Ser Pro 275 280 285 Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu
Phe Pro Pro Lys 290 295 300 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr
Pro Glu Val Thr Cys Val 305 310 315 320 Val Val Asp Val Ser His Glu
Asp Pro Glu Val Lys Phe Asn Trp Tyr 325 330 335 Val Asp Gly Val Glu
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 340 345 350 Gln Tyr Asn
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 355 360 365 Gln
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 370 375
380 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
385 390 395 400 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg
Asp Glu Leu 405 410 415 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
Lys Gly Phe Tyr Pro 420 425 430 Ser Asp Ile Ala Val Glu Trp Glu Ser
Asn Gly Gln Pro Glu Asn Asn 435 440 445 Tyr Lys Thr Thr Pro Pro Val
Leu Asp Ser Asp Gly Ser Phe Phe Leu 450 455 460 Tyr Ser Lys Leu Thr
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 465 470 475 480 Phe Ser
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 485 490 495
Lys Ser Leu Ser Leu Ser Pro Gly Lys 500 505 <210> SEQ ID NO
204 <211> LENGTH: 1498 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: G28-1 LH SMIP (DNA) <400> SEQUENCE: 204
aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca
60 gataccaccg gtgacatcca gatgactcag tctccagcct ccctatctgc
atctgtggga 120 gagactgtca ccatcacatg tcgaacaagt gaaaatgttt
acagttattt ggcttggtat 180 cagcagaaac agggaaaatc tcctcagctc
ctggtctctt ttgcaaaaac cttagcagaa 240 ggtgtgccat caaggttcag
tggcagtgga tcaggcacac agttttctct gaagatcagc 300 agcctgcagc
ctgaagattc tggaagttat ttctgtcaac atcattccga taatccgtgg 360
acgttcggtg gaggcaccga actggagatc aaaggtggcg gtggctcggg cggtggtggg
420 tcgggtggcg gcggatctgc tagcgcagtc cagctgcagc agtctggacc
tgagctggaa 480 aagcctggcg cttcagtgaa gatttcctgc aaggcttctg
gttactcatt cactggctac 540 aatatgaact gggtgaagca gaataatgga
aagagccttg agtggattgg aaatattgat 600 ccttattatg gtggtactac
ctacaaccgg aagttcaagg gcaaggccac attgactgta 660 gacaaatcct
ccagcacagc ctacatgcag ctcaagagtc tgacatctga ggactctgca 720
gtctattact gtgcaagatc ggtcggccct atggactact ggggtcaagg aacctcagtc
780 accgtctcga gcgagcccaa atcttctgac aaaactcaca catgcccacc
gtgcccagca 840 cctgaactcc tgggtggacc gtcagtcttc ctcttccccc
caaaacccaa ggacaccctc 900 atgatctccc ggacccctga ggtcacatgc
gtggtggtgg acgtgagcca cgaagaccct 960 gaggtcaagt tcaactggta
cgtggacggc gtggaggtgc ataatgccaa gacaaagccg 1020 cgggaggagc
agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag 1080
gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc
1140 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt
gtacaccctg 1200 cccccatccc gggatgagct gaccaagaac caggtcagcc
tgacctgcct ggtcaaaggc 1260 ttctatccaa gcgacatcgc cgtggagtgg
gagagcaatg ggcagccgga gaacaactac 1320 aagaccacgc ctcccgtgct
ggactccgac ggctccttct tcctctacag caagctcacc 1380 gtggacaaga
gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct 1440
ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaagtg actctaga
1498 <210> SEQ ID NO 205 <211> LENGTH: 492 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: G28-1 LH SMIP (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(20) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (21)..(127) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(128)..(144) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(145)..(260) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(261)..(275) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 205 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser
Pro Ala Ser Leu Ser 20 25 30 Ala Ser Val Gly Glu Thr Val Thr Ile
Thr Cys Arg Thr Ser Glu Asn 35 40 45 Val Tyr Ser Tyr Leu Ala Trp
Tyr Gln Gln Lys Gln Gly Lys Ser Pro 50 55 60 Gln Leu Leu Val Ser
Phe Ala Lys Thr Leu Ala Glu Gly Val Pro Ser 65 70 75 80 Arg Phe Ser
Gly Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser 85 90 95 Ser
Leu Gln Pro Glu Asp Ser Gly Ser Tyr Phe Cys Gln His His Ser 100 105
110 Asp Asn Pro Trp Thr Phe Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly
115 120 125 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Ala Ser 130 135 140 Ala Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu
Lys Pro Gly Ala 145 150 155 160 Ser Val Lys Ile Ser Cys Lys Ala Ser
Gly Tyr Ser Phe Thr Gly Tyr 165 170 175 Asn Met Asn Trp Val Lys Gln
Asn Asn Gly Lys Ser Leu Glu Trp Ile 180 185 190 Gly Asn Ile Asp Pro
Tyr Tyr Gly Gly Thr Thr Tyr Asn Arg Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 225 230
235 240 Ala Arg Ser Val Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr Ser
Val 245 250 255 Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr His
Thr Cys Pro 260 265 270 Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
Ser Val Phe Leu Phe 275 280 285 Pro Pro Lys Pro Lys Asp Thr Leu Met
Ile Ser Arg Thr Pro Glu Val 290 295 300 Thr Cys Val Val Val Asp Val
Ser His Glu Asp Pro Glu Val Lys Phe 305 310 315 320 Asn Trp Tyr Val
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 325 330 335 Arg Glu
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 340 345 350
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 355
360 365 Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
Ala 370 375 380 Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
Pro Ser Arg 385 390 395 400 Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
Thr Cys Leu Val Lys Gly 405 410 415 Phe Tyr Pro Ser Asp Ile Ala Val
Glu Trp Glu Ser Asn Gly Gln Pro 420 425 430 Glu Asn Asn Tyr Lys Thr
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 435 440 445 Phe Phe Leu Tyr
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 450 455 460 Gly Asn
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 465 470 475
480 Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490
<210> SEQ ID NO 206 <211> LENGTH: 1522 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: G28-1 HL SMIP (DNA)
<400> SEQUENCE: 206 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtgcggtcca
gctgcagcag tctggacctg agtcggaaaa gcctggcgct 120 tcagtgaaga
tttcctgcaa ggcttctggt tactcattca ctggctacaa tatgaactgg 180
gtgaagcaga ataatggaaa gagccttgag tggattggaa atattgatcc ttattatggt
240 ggtactacct acaaccggaa gttcaagggc aaggccacat tgactgtaga
caaatcctcc 300 ggcacagcct acatgcagct caagagtctg acatctgagg
actctgcagt ctattactgt 360
gcaagatcgg tcggccctat ggactactgg ggtcaaggaa cctcagtcac cgtctcttct
420 ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatcaggagg
aggcgggagt 480 gctagcgaca tccagatgac tcagtctcca gcctccctat
ctgcatctgt gggagagact 540 gtcaccatca catgtcgaac aagtgaaaat
gtttacagtt atttggcttg gtatcagcag 600 aaacagggaa aatctcctca
gctcctggtc tcttttgcaa aaaccttagc agaaggtgtg 660 ccatcaaggt
tcagtggcag tggatcaggc acacagtttt ctctgaagat cagcagcctg 720
cagcctgaag attctggaag ttatttctgt caacatcatt ccgataatcc gtggacgttc
780 ggtggaggca ccgaactgga gatcaaaggc tcgagcgagc ccaaatcttc
tgacaaaact 840 cacacatgcc caccgtgccc agcacctgaa ctcctgggtg
gaccgtcagt cttcctcttc 900 cccccaaaac ccaaggacac cctcatgatc
tcccggaccc ctgaggtcac atgcgtggtg 960 gtggacgtga gccacgaaga
ccctgaggtc aagttcaact ggtacgtgga cggcgtggag 1020 gtgcataatg
ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc 1080
agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc
1140 tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa
agggcagccc 1200 cgagaaccac aggtgtacac cctgccccca tcccgggatg
agctgaccaa gaaccaggtc 1260 agcctgacct gcctggtcaa aggcttctat
ccaagcgaca tcgccgtgga gtgggagagc 1320 aatgggcagc cggagaacaa
ctacaagacc acgcctcccg tgctggactc cgacggctcc 1380 ttcttcctct
acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc 1440
tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg
1500 tctccgggta agtgactcta ga 1522 <210> SEQ ID NO 207
<211> LENGTH: 500 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: G28-1 HL SMIP (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(136)
<223> OTHER INFORMATION: VH <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (137)..(158)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(159)..(266) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(268)..(283) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 207 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Ala Val Gln Leu Gln Gln Ser
Gly Pro Glu Ser Glu 20 25 30 Lys Pro Gly Ala Ser Val Lys Ile Ser
Cys Lys Ala Ser Gly Tyr Ser 35 40 45 Phe Thr Gly Tyr Asn Met Asn
Trp Val Lys Gln Asn Asn Gly Lys Ser 50 55 60 Leu Glu Trp Ile Gly
Asn Ile Asp Pro Tyr Tyr Gly Gly Thr Thr Tyr 65 70 75 80 Asn Arg Lys
Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser 85 90 95 Gly
Thr Ala Tyr Met Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala 100 105
110 Val Tyr Tyr Cys Ala Arg Ser Val Gly Pro Met Asp Tyr Trp Gly Gln
115 120 125 Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
Gly Gly 130 135 140 Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
Ala Ser Asp Ile 145 150 155 160 Gln Met Thr Gln Ser Pro Ala Ser Leu
Ser Ala Ser Val Gly Glu Thr 165 170 175 Val Thr Ile Thr Cys Arg Thr
Ser Glu Asn Val Tyr Ser Tyr Leu Ala 180 185 190 Trp Tyr Gln Gln Lys
Gln Gly Lys Ser Pro Gln Leu Leu Val Ser Phe 195 200 205 Ala Lys Thr
Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 210 215 220 Ser
Gly Thr Gln Phe Ser Leu Lys Ile Ser Ser Leu Gln Pro Glu Asp 225 230
235 240 Ser Gly Ser Tyr Phe Cys Gln His His Ser Asp Asn Pro Trp Thr
Phe 245 250 255 Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly Ser Ser Glu
Pro Lys Ser 260 265 270 Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro
Ala Pro Glu Leu Leu 275 280 285 Gly Gly Pro Ser Val Phe Leu Phe Pro
Pro Lys Pro Lys Asp Thr Leu 290 295 300 Met Ile Ser Arg Thr Pro Glu
Val Thr Cys Val Val Val Asp Val Ser 305 310 315 320 His Glu Asp Pro
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 325 330 335 Val His
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 340 345 350
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 355
360 365 Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
Pro 370 375 380 Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
Glu Pro Gln 385 390 395 400 Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
Leu Thr Lys Asn Gln Val 405 410 415 Ser Leu Thr Cys Leu Val Lys Gly
Phe Tyr Pro Ser Asp Ile Ala Val 420 425 430 Glu Trp Glu Ser Asn Gly
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 435 440 445 Pro Val Leu Asp
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 450 455 460 Val Asp
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 465 470 475
480 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
485 490 495 Ser Pro Gly Lys 500 <210> SEQ ID NO 208
<211> LENGTH: 1522 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: G19-4 LH SMIP (DNA) <400> SEQUENCE: 208
aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca
60 gataccaccg gtgacatcca gatgacacag actacatcct ccctgtctgc
ctctctggga 120 gacagagtca ccatcagttg cagggcaagt caggacattc
gcaattattt aaactggtat 180 cagcagaaac cagatggaac tgttaaactc
ctgatctact acacatcaag attacactca 240 ggagtcccat caaggttcag
tggcagtggg tctggaacag attattctct caccattgcc 300 aacctgcaac
cagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtgg 360
acgttcggtg gaggcaccaa actggtaacc aaacggggtg gcggtggctc gggcggtggt
420 ggatctggag gaggtgggag cgctagcgag gtccagctgc aacagtctgg
acctgaactg 480 gtgaagcctg gagcttcaat gaagatttcc tgcaaggcct
ctggttactc attcactggc 540 tacatcgtga actggctgaa gcagagccat
ggaaagaacc ttgagtggat tggacttatt 600 aatccataca aaggtcttac
tacctacaac cagaaattca agggcaaggc cacattaact 660 gtagacaagt
catccagcac agcctacatg gagctcctca gtctgacatc tgaagactct 720
gcagtctatt actgtgcaag atctgggtac tatggtgact cggactggta cttcgatgtc
780 tggggcgcag ggaccacggt caccgtctcc tcgagcgagc ccaaatcttc
tgacaaaact 840 cacacatgcc caccgtgccc agcacctgaa ctcctgggtg
gaccgtcagt cttcctcttc 900 cccccaaaac ccaaggacac cctcatgatc
tcccggaccc ctgaggtcac atgcgtggtg 960 gtggacgtga gccacgaaga
ccctgaggtc aagttcaact ggtacgtgga cggcgtggag 1020 gtgcataatg
ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc 1080
agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc
1140 tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa
agggcagccc 1200 cgagaaccac aggtgtacac cctgccccca tcccgggatg
agctgaccaa gaaccaggtc 1260 agcctgacct gcctggtcaa aggcttctat
ccaagcgaca tcgccgtgga gtgggagagc 1320 aatgggcagc cggagaacaa
ctacaagacc acgcctcccg tgctggactc cgacggctcc 1380 ttcttcctct
acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc 1440
tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg
1500 tctccgggta agtgactcta ga 1522 <210> SEQ ID NO 209
<211> LENGTH: 500 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: G19-4 LH SMIP (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(128)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (129)..(145)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(146)..(267)
<223> OTHER INFORMATION: VH <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (268)..(282)
<223> OTHER INFORMATION: Hinge <400> SEQUENCE: 209 Met
Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10
15 Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser
20 25 30 Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser
Gln Asp 35 40 45 Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro
Asp Gly Thr Val 50 55 60 Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu
His Ser Gly Val Pro Ser 65 70 75 80 Arg Phe Ser Gly Ser Gly Ser Gly
Thr Asp Tyr Ser Leu Thr Ile Ala 85 90 95 Asn Leu Gln Pro Glu Asp
Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn 100 105 110 Thr Leu Pro Trp
Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg 115 120 125 Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala 130 135 140
Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly 145
150 155 160 Ala Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe
Thr Gly 165 170 175 Tyr Ile Val Asn Trp Leu Lys Gln Ser His Gly Lys
Asn Leu Glu Trp 180 185 190 Ile Gly Leu Ile Asn Pro Tyr Lys Gly Leu
Thr Thr Tyr Asn Gln Lys 195 200 205 Phe Lys Gly Lys Ala Thr Leu Thr
Val Asp Lys Ser Ser Ser Thr Ala 210 215 220 Tyr Met Glu Leu Leu Ser
Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 225 230 235 240 Cys Ala Arg
Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val 245 250 255 Trp
Gly Ala Gly Thr Thr Val Thr Val Ser Ser Ser Glu Pro Lys Ser 260 265
270 Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu
275 280 285 Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
Thr Leu 290 295 300 Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val
Val Asp Val Ser 305 310 315 320 His Glu Asp Pro Glu Val Lys Phe Asn
Trp Tyr Val Asp Gly Val Glu 325 330 335 Val His Asn Ala Lys Thr Lys
Pro Arg Glu Glu Gln Tyr Asn Ser Thr 340 345 350 Tyr Arg Val Val Ser
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 355 360 365 Gly Lys Glu
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 370 375 380 Ile
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 385 390
395 400 Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
Val 405 410 415 Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
Ile Ala Val 420 425 430 Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
Tyr Lys Thr Thr Pro 435 440 445 Pro Val Leu Asp Ser Asp Gly Ser Phe
Phe Leu Tyr Ser Lys Leu Thr 450 455 460 Val Asp Lys Ser Arg Trp Gln
Gln Gly Asn Val Phe Ser Cys Ser Val 465 470 475 480 Met His Glu Ala
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 485 490 495 Ser Pro
Gly Lys 500 <210> SEQ ID NO 210 <211> LENGTH: 1525
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: G19-4 HL SMIP
(DNA) <400> SEQUENCE: 210 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtgaggtcca
gctgcaacag tctggacctg aactggtgaa gcctggagct 120 tcaatgaaga
tttcctgcaa ggcctctggt tactcattca ctggctacat cgtgaactgg 180
ctgaagcaga gccatggaaa gaaccttgag tggattggac ttattaatcc atacaaaggt
240 cttactacct acaaccagaa attcaagggc aaggccacat tgactgtaga
caagtcatcc 300 agcacagcct acatggagct cctcagtctg acatctgaag
actctgcagt ctattactgt 360 gcaagatctg ggtactatgg tgactcagac
tggtacttcg atgtctgggg cgcagggacc 420 acagtcaccg tctcctctgg
cggcggtggc tcgggcggtg gtggatctgg aggaggtggg 480 agcgctagcg
acatccagat gacacagact acatcctccc tgtctgcctc tctgggagac 540
agagtcacca tcagttgcag ggcaagtcag gacattcgca attatttgaa ctggtatcag
600 cagaaaccag atggaactgt taaactcctg atctactaca catcaagact
acactcagga 660 gtcccatcaa ggttcagtgg cagtgggtct ggaacagatt
attctctcac cattgccaac 720 ctgcaaccag aagatattgc cacttacttt
tgccaacagg gtaatacact tccctggacg 780 ttcggtggag ggaccaaact
ggtgaccaaa cgctcgagcg agcccaaatc ttctgacaaa 840 actcacacat
gcccaccgtg cccagcacct gaactcctgg gtggaccgtc agtcttcctc 900
ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg
960 gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
ggacggcgtg 1020 gaggtgcata atgccaagac aaagccgcgg gaggagcagt
acaacagcac gtaccgtgtg 1080 gtcagcgtcc tcaccgtcct gcaccaggac
tggctgaatg gcaaggagta caagtgcaag 1140 gtctccaaca aagccctccc
agcccccatc gagaaaacca tctccaaagc caaagggcag 1200 ccccgagaac
cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag 1260
gtcagcctga cctgcctggt caaaggcttc tatccaagcg acatcgccgt ggagtgggag
1320 agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
ctccgacggc 1380 tccttcttcc tctacagcaa gctcaccgtg gacaagagca
ggtggcagca ggggaacgtc 1440 ttctcatgct ccgtgatgca tgaggctctg
cacaaccact acacgcagaa gagcctctcc 1500 ctgtctccgg gtaagtgact ctaga
1525 <210> SEQ ID NO 211 <211> LENGTH: 501 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: G19-4 HL SMIP (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(20) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (21)..(142) <223> OTHER INFORMATION: VH <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(159) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(160)..(267) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(270)..(284) <223> OTHER INFORMATION: Hinge <400>
SEQUENCE: 211 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu
Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Glu Val Gln Leu Gln Gln Ser
Gly Pro Glu Leu Val 20 25 30 Lys Pro Gly Ala Ser Met Lys Ile Ser
Cys Lys Ala Ser Gly Tyr Ser 35 40 45 Phe Thr Gly Tyr Ile Val Asn
Trp Leu Lys Gln Ser His Gly Lys Asn 50 55 60 Leu Glu Trp Ile Gly
Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr 65 70 75 80 Asn Gln Lys
Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser 85 90 95 Ser
Thr Ala Tyr Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala 100 105
110 Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr
115 120 125 Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser
Gly Gly 130 135 140 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ala Ser Asp 145 150 155 160 Ile Gln Met Thr Gln Thr Thr Ser Ser
Leu Ser Ala Ser Leu Gly Asp 165 170 175 Arg Val Thr Ile Ser Cys Arg
Ala Ser Gln Asp Ile Arg Asn Tyr Leu 180 185 190 Asn Trp Tyr Gln Gln
Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 195 200 205 Tyr Thr Ser
Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 210 215 220 Gly
Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu 225 230
235 240 Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp
Thr 245 250 255 Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg Ser Ser
Glu Pro Lys 260 265 270 Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys
Pro Ala Pro Glu Leu 275 280 285 Leu Gly Gly Pro Ser Val Phe Leu Phe
Pro Pro Lys Pro Lys Asp Thr
290 295 300 Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
Asp Val 305 310 315 320 Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
Tyr Val Asp Gly Val 325 330 335 Glu Val His Asn Ala Lys Thr Lys Pro
Arg Glu Glu Gln Tyr Asn Ser 340 345 350 Thr Tyr Arg Val Val Ser Val
Leu Thr Val Leu His Gln Asp Trp Leu 355 360 365 Asn Gly Lys Glu Tyr
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 370 375 380 Pro Ile Glu
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 385 390 395 400
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 405
410 415 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
Ala 420 425 430 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr
Lys Thr Thr 435 440 445 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
Leu Tyr Ser Lys Leu 450 455 460 Thr Val Asp Lys Ser Arg Trp Gln Gln
Gly Asn Val Phe Ser Cys Ser 465 470 475 480 Val Met His Glu Ala Leu
His Asn His Tyr Thr Gln Lys Ser Leu Ser 485 490 495 Leu Ser Pro Gly
Lys 500 <210> SEQ ID NO 212 <211> LENGTH: 2328
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-STD1-2e12HL (DNA) <400> SEQUENCE: 212 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 atgcatgagg
ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500
aattatggtg gcggtggctc gggcggtggt ggatctggag gaggtgggag tgggaattct
1560 caggtgcagc tgaaggagtc aggacctggc ctggtggcgc cctcacagag
cctgtccatc 1620 acatgcaccg tctcagggtt ctcattaacc ggctatggtg
taaactgggt tcgccagcct 1680 ccaggaaagg gtctggagtg gctgggaatg
atatggggtg atggaagcac agactataat 1740 tcagctctca aatccagact
atcgatcacc aaggacaact ccaagagcca agttttctta 1800 aaaatgaaca
gtctgcaaac tgatgacaca gccagatact actgtgctcg agatggttat 1860
agtaactttc attactatgt tatggactac tggggtcaag gaacctcagt caccgtctcc
1920 tctgggggtg gaggctctgg tggcggtgga tccggcggag gtgggtcggg
tggcggcgga 1980 tctgacattg tgctcaccca atctccagct tctttggctg
tgtctctagg tcagagagcc 2040 accatctcct gcagagccag tgaaagtgtt
gaatattatg tcacaagttt aatgcagtgg 2100 taccaacaga aaccaggaca
gccacccaaa ctcctcatct ctgctgctag caacgtagaa 2160 tctggggtcc
ctgccaggtt tagtggcagt gggtctggga cagactttag cctcaacatc 2220
catcctgtgg aggaggatga tattgcaatg tatttctgtc agcaaagtag gaaggttcca
2280 tggacgttcg gtggaggcac caagctggaa atcaaacgtt aatctaga 2328
<210> SEQ ID NO 213 <211> LENGTH: 769 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: n2H7sssIgG1-STD1-2e12HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(20) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (21)..(127) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(128)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(516) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (517)..(637) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (638)..(657) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (658)..(769) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 213 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys
405 410 415 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
Glu Ser 420 425 430 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
Pro Val Leu Asp 435 440 445 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
Leu Thr Val Asp Lys Ser 450 455 460 Arg Trp Gln Gln Gly Asn Val Phe
Ser Cys Ser Val Met His Glu Ala 465 470 475 480 Leu His Asn His Tyr
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495 Asn Tyr Gly
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 500 505 510 Ser
Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val 515 520
525 Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser
530 535 540 Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly
Lys Gly 545 550 555 560 Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly
Ser Thr Asp Tyr Asn 565 570 575 Ser Ala Leu Lys Ser Arg Leu Ser Ile
Thr Lys Asp Asn Ser Lys Ser 580 585 590 Gln Val Phe Leu Lys Met Asn
Ser Leu Gln Thr Asp Asp Thr Ala Arg 595 600 605 Tyr Tyr Cys Ala Arg
Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met 610 615 620 Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly 625 630 635 640
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 645
650 655 Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser
Leu 660 665 670 Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser
Val Glu Tyr 675 680 685 Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln
Lys Pro Gly Gln Pro 690 695 700 Pro Lys Leu Leu Ile Ser Ala Ala Ser
Asn Val Glu Ser Gly Val Pro 705 710 715 720 Ala Arg Phe Ser Gly Ser
Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile 725 730 735 His Pro Val Glu
Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser 740 745 750 Arg Lys
Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 755 760 765
Arg <210> SEQ ID NO 214 <211> LENGTH: 2313 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: n2H7sssIgG1-STD2-2e12LH
(DNA) <400> SEQUENCE: 214 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtcaaattgt
tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca
caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180
cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga
240 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac
aatcagcaga 300 gtggaggctg aagatgctgc cacttattac tgccagcagt
ggagttttaa cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa
gatggcggtg gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca
ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag
tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540
cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga
600 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac
tgtagacaaa 660 tcctccagca cagcctacat gcagctcagc agcctgacat
ctgaagactc tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac
tcttactggt acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc
gagcgagccc aaatcttctg acaaaactca cacatcccca 840 ccgagcccag
cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900
aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
960 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
gcataatgcc 1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc
gtgtggtcag cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag
gagtacaagt gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa
aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc
tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260
ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
1320 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
cttcctctac 1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga
acgtcttctc atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg
cagaagagcc tctccctgtc tccgggtaag 1500 aattatggtg gcggtggctc
gggcggtggt ggatctggag gaggtgggag tgggaattct 1560 gacattgtgc
tcacccaatc tccagcttct ttggctgtgt ctctaggtca gagagccacc 1620
atctcctgca gagccagtga aagtgttgaa tattatgtca caagtttaat gcagtggtac
1680 caacagaaac caggacagcc acccaaactc ctcatctctg ctgctagcaa
cgtagaatct 1740 ggggtccctg ccaggtttag tggcagtggg tctgggacag
actttagcct caacatccat 1800 cctgtggagg aggatgatat tgcaatgtat
ttctgtcagc aaagtaggaa ggttccatgg 1860 acgttcggtg gaggcaccaa
gctggaaatc aaacggggtg gcggtggatc cggcggaggt 1920 gggtcgggtg
gcggcggatc tcaggtgcag ctgaaggagt caggacctgg cctggtggcg 1980
ccctcacaga gcctgtccat cacatgcacc gtctcagggt tctcattaac cggctatggt
2040 gtaaactggg ttcgccagcc tccaggaaag ggtctggagt ggctgggaat
gatatggggt 2100 gatggaagca cagactataa ttcagctctc aaatccagac
tatcgatcac caaggacaac 2160 tccaagagcc aagttttctt aaaaatgaac
agtctgcaaa ctgatgacac agccagatac 2220 tactgtgctc gagatggtta
tagtaacttt cattactatg ttatggacta ctggggtcaa 2280 ggaacctcag
tcaccgtctc ctcttaatct aga 2313 <210> SEQ ID NO 215
<211> LENGTH: 764 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: n2H7sssIgG1-STD2-2e12LH (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(20)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(126) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(516) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (517)..(628) <223> OTHER INFORMATION: VL2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (629)..(643) <223> OTHER INFORMATION: Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (644)..(764) <223> OTHER INFORMATION: VH2
<400> SEQUENCE: 215 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255
Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260
265 270 His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro
Ser 275 280 285 Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
Ile Ser Arg 290 295 300 Thr Pro Glu Val Thr Cys Val Val Val Asp Val
Ser His Glu Asp Pro 305 310 315 320 Glu Val Lys Phe Asn Trp Tyr Val
Asp Gly Val Glu Val His Asn Ala 325 330 335 Lys Thr Lys Pro Arg Glu
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 340 345 350 Ser Val Leu Thr
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365 Lys Cys
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 385
390 395 400 Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
Thr Cys 405 410 415 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
Glu Trp Glu Ser 420 425 430 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
Thr Pro Pro Val Leu Asp 435 440 445 Ser Asp Gly Ser Phe Phe Leu Tyr
Ser Lys Leu Thr Val Asp Lys Ser 450 455 460 Arg Trp Gln Gln Gly Asn
Val Phe Ser Cys Ser Val Met His Glu Ala 465 470 475 480 Leu His Asn
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495 Asn
Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 500 505
510 Ser Gly Asn Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala
515 520 525 Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser
Glu Ser 530 535 540 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr
Gln Gln Lys Pro 545 550 555 560 Gly Gln Pro Pro Lys Leu Leu Ile Ser
Ala Ala Ser Asn Val Glu Ser 565 570 575 Gly Val Pro Ala Arg Phe Ser
Gly Ser Gly Ser Gly Thr Asp Phe Ser 580 585 590 Leu Asn Ile His Pro
Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 595 600 605 Gln Gln Ser
Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 610 615 620 Glu
Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 625 630
635 640 Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val
Ala 645 650 655 Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly
Phe Ser Leu 660 665 670 Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro
Pro Gly Lys Gly Leu 675 680 685 Glu Trp Leu Gly Met Ile Trp Gly Asp
Gly Ser Thr Asp Tyr Asn Ser 690 695 700 Ala Leu Lys Ser Arg Leu Ser
Ile Thr Lys Asp Asn Ser Lys Ser Gln 705 710 715 720 Val Phe Leu Lys
Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr 725 730 735 Tyr Cys
Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp 740 745 750
Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 755 760 <210>
SEQ ID NO 216 <211> LENGTH: 2048 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: n2H7sssIgG1-H1-2e12HL (DNA)
<400> SEQUENCE: 216 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtcaaattgt
tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca
caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180
cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga
240 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac
aatcagcaga 300 gtggaggctg aagatgctgc cacttattac tgccagcagt
ggagttttaa cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa
gatggcggtg gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca
ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag
tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540
cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga
600 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac
tgtagacaaa 660 tcctccagca cagcctacat gcagctcagc agcctgacat
ctgaagactc tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac
tcttactggt acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc
gagcgagccc aaatcttctg acaaaactca cacatcccca 840 ccgagcccag
cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900
aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
960 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
gcataatgcc 1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc
gtgtggtcag cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag
gagtacaagt gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa
aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc
tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260
ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
1320 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
cttcctctac 1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga
acgtcttctc atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg
cagaagagcc tctccctgtc tccgggtaag 1500 aattctcagg tgcagctgaa
ggagtcagga cctggcctgg tggcgccctc acagagcctg 1560 tccatcacat
gcaccgtctc agggttctca ttaaccggct atggtgtaaa ctgggttcgc 1620
cagcctccag gaaagggtct ggagtggctg ggaatgatat ggggtgatgg aagcacagac
1680 tataattcag ctctcaaatc cagactatcg atcaccaagg acaactccaa
gagccaagtt 1740 ttcttaaaaa tgaacagtct gcaaactgat gacacagcca
gatactactg tgctcgagat 1800 ggttatagta actttcatta ctatgttatg
gactactggg gtcaaggaac ctcagtcacc 1860 gtctcctctg ggggtggagg
ctctggtggc ggtggatccg gcggaggtgg gtcgggtggc 1920 ggcggatctg
acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 1980
agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg
2040 cagtggta 2048 <210> SEQ ID NO 217 <211> LENGTH:
751 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H1-2e12HL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(498) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (499)..(619) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (620)..(639) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (640)..(751) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 217 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160
Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165
170 175 His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly
Ala 180 185 190 Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys
Phe Lys Gly 195 200 205 Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser
Thr Ala Tyr Met Gln 210 215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser
Ala Val Tyr Phe Cys Ala Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn
Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 255 Gly Thr Thr Val
Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260 265 270 His Thr
Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290
295 300 Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
Pro 305 310 315 320 Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
Val His Asn Ala 325 330 335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
Ser Thr Tyr Arg Val Val 340 345 350 Ser Val Leu Thr Val Leu His Gln
Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro
Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410
415 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
420 425 430 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
Leu Asp 435 440 445 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
Val Asp Lys Ser 450 455 460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
Ser Val Met His Glu Ala 465 470 475 480 Leu His Asn His Tyr Thr Gln
Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495 Asn Ser Gln Val Gln
Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro 500 505 510 Ser Gln Ser
Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 515 520 525 Gly
Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 530 535
540 Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala
545 550 555 560 Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys
Ser Gln Val 565 570 575 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp
Thr Ala Arg Tyr Tyr 580 585 590 Cys Ala Arg Asp Gly Tyr Ser Asn Phe
His Tyr Tyr Val Met Asp Tyr 595 600 605 Trp Gly Gln Gly Thr Ser Val
Thr Val Ser Ser Gly Gly Gly Gly Ser 610 615 620 Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 625 630 635 640 Ile Val
Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 645 650 655
Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 660
665 670 Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro
Lys 675 680 685 Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val
Pro Ala Arg 690 695 700 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser
Leu Asn Ile His Pro 705 710 715 720 Val Glu Glu Asp Asp Ile Ala Met
Tyr Phe Cys Gln Gln Ser Arg Lys 725 730 735 Val Pro Trp Thr Phe Gly
Gly Gly Thr Lys Leu Glu Ile Lys Arg 740 745 750 <210> SEQ ID
NO 218 <211> LENGTH: 2292 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: n2H7sssIgG1-H2-2e12HL (DNA) <400>
SEQUENCE: 218 aagcttgccg ccatggaagc accagcgcag cttctcttcc
tcctgctact ctggctccca 60 gataccaccg gtcaaattgt tctctcccag
tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca caatgacttg
cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180 cagaagccag
gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240
gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga
300 gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa
cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg
gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca ggcttatcta
cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag tgaagatgtc
ctgcaaggct tctggctaca catttaccag ttacaatatg 540 cactgggtaa
agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600
aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa
660 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc
tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt
acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc gagcgagccc
aaatcttctg acaaaactca cacatcccca 840 ccgagcccag cacctgaact
cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900 aaggacaccc
tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960
cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag gagtacaagt
gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa aacaatctcc
aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc tgcccccatc
ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260 ctggtcaaag
gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320
gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac
1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg cagaagagcc
tctccctgtc tccgggtaag 1500 ggtggcggtg gctcggggaa ttctcaggtg
cagctgaagg agtcaggacc tggcctggtg 1560 gcgccctcac agagcctgtc
catcacatgc accgtctcag ggttctcatt aaccggctat 1620 ggtgtaaact
gggttcgcca gcctccagga aagggtctgg agtggctggg aatgatatgg 1680
ggtgatggaa gcacagacta taattcagct ctcaaatcca gactatcgat caccaaggac
1740 aactccaaga gccaagtttt cttaaaaatg aacagtctgc aaactgatga
cacagccaga 1800 tactactgtg ctcgagatgg ttatagtaac tttcattact
atgttatgga ctactggggt 1860 caaggaacct cagtcaccgt ctcctctggg
ggtggaggct ctggtggcgg tggatccggc 1920 ggaggtgggt cgggtggcgg
cggatctgac attgtgctca cccaatctcc agcttctttg 1980 gctgtgtctc
taggtcagag agccaccatc tcctgcagag ccagtgaaag tgttgaatat 2040
tatgtcacaa gtttaatgca gtggtaccaa cagaaaccag gacagccacc caaactcctc
2100 atctctgctg ctagcaacgt agaatctggg gtccctgcca ggtttagtgg
cagtgggtct 2160 gggacagact ttagcctcaa catccatcct gtggaggagg
atgatattgc aatgtatttc 2220 tgtcagcaaa gtaggaaggt tccatggacg
ttcggtggag gcaccaagct ggaaatcaaa 2280 cgttaatcta ga 2292
<210> SEQ ID NO 219 <211> LENGTH: 757 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: n2H7sssIgG1-H2-2e12HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(20) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (21)..(126) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(504) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (505)..(625) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (626)..(645) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (646)..(757) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 219 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30
Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35
40 45 Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro
Lys 50 55 60 Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val
Pro Ala Arg 65 70 75 80 Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser
Leu Thr Ile Ser Arg 85 90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr
Tyr Cys Gln Gln Trp Ser Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala
Gly Thr Lys Leu Glu Leu Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly
Gly Gly Ser Gly Gly Gly Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln
Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala Ser Val 145 150 155 160
Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165
170 175 His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly
Ala 180 185 190 Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys
Phe Lys Gly 195 200 205 Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser
Thr Ala Tyr Met Gln 210 215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser
Ala Val Tyr Phe Cys Ala Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn
Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 255 Gly Thr Thr Val
Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260 265 270 His Thr
Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290
295 300 Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
Pro 305 310 315 320 Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
Val His Asn Ala 325 330 335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
Ser Thr Tyr Arg Val Val 340 345 350 Ser Val Leu Thr Val Leu His Gln
Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro
Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410
415 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
420 425 430 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
Leu Asp 435 440 445 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
Val Asp Lys Ser 450 455 460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
Ser Val Met His Glu Ala 465 470 475 480 Leu His Asn His Tyr Thr Gln
Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495 Gly Gly Gly Gly Ser
Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly 500 505 510 Pro Gly Leu
Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val 515 520 525 Ser
Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 530 535
540 Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser
545 550 555 560 Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile
Thr Lys Asp 565 570 575 Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn
Ser Leu Gln Thr Asp 580 585 590 Asp Thr Ala Arg Tyr Tyr Cys Ala Arg
Asp Gly Tyr Ser Asn Phe His 595 600 605 Tyr Tyr Val Met Asp Tyr Trp
Gly Gln Gly Thr Ser Val Thr Val Ser 610 615 620 Ser Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly
Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu 645 650 655
Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu 660
665 670 Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln
Lys 675 680 685 Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser
Asn Val Glu 690 695 700 Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly
Ser Gly Thr Asp Phe 705 710 715 720 Ser Leu Asn Ile His Pro Val Glu
Glu Asp Asp Ile Ala Met Tyr Phe 725 730 735 Cys Gln Gln Ser Arg Lys
Val Pro Trp Thr Phe Gly Gly Gly Thr Lys 740 745 750 Leu Glu Ile Lys
Arg 755 <210> SEQ ID NO 220 <211> LENGTH: 2298
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H3-2e12HL (DNA) <400> SEQUENCE: 220 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 atgcatgagg
ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500
aattatggtg gcggtggctc tgggaattct caggtgcagc tgaaggagtc aggacctggc
1560 ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt
ctcattaacc 1620 ggctatggtg taaactgggt tcgccagcct ccaggaaagg
gtctggagtg gctgggaatg 1680 atatggggtg atggaagcac agactataat
tcagctctca aatccagact atcgatcacc 1740 aaggacaact ccaagagcca
agttttctta aaaatgaaca gtctgcaaac tgatgacaca 1800 gccagatact
actgtgctcg agatggttat agtaactttc attactatgt tatggactac 1860
tggggtcaag gaacctcagt caccgtctcc tctgggggtg gaggctctgg tggcggtgga
1920 tccggcggag gtgggtcggg tggcggcgga tctgacattg tgctcaccca
atctccagct 1980 tctttggctg tgtctctagg tcagagagcc accatctcct
gcagagccag tgaaagtgtt 2040 gaatattatg tcacaagttt aatgcagtgg
taccaacaga aaccaggaca gccacccaaa 2100 ctcctcatct ctgctgctag
caacgtagaa tctggggtcc ctgccaggtt tagtggcagt 2160 gggtctggga
cagactttag cctcaacatc catcctgtgg aggaggatga tattgcaatg 2220
tatttctgtc agcaaagtag gaaggttcca tggacgttcg gtggaggcac caagctggaa
2280 atcaaacgtt aatctaga 2298 <210> SEQ ID NO 221 <211>
LENGTH: 759 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H3-2e12HL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220>
FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(506) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (507)..(627) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (628)..(647) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (648)..(759) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 221 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser Gln
Val Gln Leu Lys Glu 500 505 510 Ser Gly Pro Gly Leu Val Ala Pro Ser
Gln Ser Leu Ser Ile Thr Cys 515 520 525 Thr Val Ser Gly Phe Ser Leu
Thr Gly Tyr Gly Val Asn Trp Val Arg 530 535 540 Gln Pro Pro Gly Lys
Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp 545 550 555 560 Gly Ser
Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr 565 570 575
Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 580
585 590 Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser
Asn 595 600 605 Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr
Ser Val Thr 610 615 620 Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly 625 630 635 640 Gly Ser Gly Gly Gly Gly Ser Asp
Ile Val Leu Thr Gln Ser Pro Ala 645 650 655 Ser Leu Ala Val Ser Leu
Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 660 665 670 Ser Glu Ser Val
Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 675 680 685 Gln Lys
Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 690 695 700
Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 705
710 715 720 Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile
Ala Met 725 730 735 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr
Phe Gly Gly Gly 740 745 750 Thr Lys Leu Glu Ile Lys Arg 755
<210> SEQ ID NO 222 <211> LENGTH: 2307 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: n2H7sssIgG1-H4-2e12HL (DNA)
<400> SEQUENCE: 222 aagcttgccg ccatggaagc accagcgcag
cttctcttcc tcctgctact ctggctccca 60 gataccaccg gtcaaattgt
tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 gagaaggtca
caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180
cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga
240 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac
aatcagcaga 300 gtggaggctg aagatgctgc cacttattac tgccagcagt
ggagttttaa cccacccacg 360 ttcggtgctg ggaccaagct ggagctgaaa
gatggcggtg gctcgggcgg tggtggatct 420 ggaggaggtg gagctagcca
ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 ggggcctcag
tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540
cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga
600 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac
tgtagacaaa 660 tcctccagca cagcctacat gcagctcagc agcctgacat
ctgaagactc tgcggtctat 720 ttctgtgcaa gagtggtgta ctatagtaac
tcttactggt acttcgatgt ctggggcaca 780 gggaccacgg tcaccgtctc
gagcgagccc aaatcttctg acaaaactca cacatcccca 840 ccgagcccag
cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900
aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
960 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
gcataatgcc 1020 aagacaaagc cgcgggagga gcagtacaac agcacgtacc
gtgtggtcag cgtcctcacc 1080 gtcctgcacc aggactggct gaatggcaag
gagtacaagt gcaaggtctc caacaaagcc 1140 ctcccagccc ccatcgagaa
aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200 gtgtacaccc
tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260
ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg
1320 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
cttcctctac 1380 agcaagctca ccgtggacaa gagcaggtgg cagcagggga
acgtcttctc atgctccgtg 1440 atgcatgagg ctctgcacaa ccactacacg
cagaagagcc tctccctgtc tccgggtaag 1500 ggtggcggtg gctcgggcgg
tggtggatct gggaattctc aggtgcagct gaaggagtca 1560 ggacctggcc
tggtggcgcc ctcacagagc ctgtccatca catgcaccgt ctcagggttc 1620
tcattaaccg gctatggtgt aaactgggtt cgccagcctc caggaaaggg tctggagtgg
1680 ctgggaatga tatggggtga tggaagcaca gactataatt cagctctcaa
atccagacta 1740 tcgatcacca aggacaactc caagagccaa gttttcttaa
aaatgaacag tctgcaaact 1800 gatgacacag ccagatacta ctgtgctcga
gatggttata gtaactttca ttactatgtt 1860 atggactact ggggtcaagg
aacctcagtc accgtctcct ctgggggtgg aggctctggt 1920 ggcggtggat
ccggcggagg tgggtcgggt ggcggcggat ctgacattgt gctcacccaa 1980
tctccagctt ctttggctgt gtctctaggt cagagagcca ccatctcctg cagagccagt
2040 gaaagtgttg aatattatgt cacaagttta atgcagtggt accaacagaa
accaggacag 2100 ccacccaaac tcctcatctc tgctgctagc aacgtagaat
ctggggtccc tgccaggttt 2160 agtggcagtg ggtctgggac agactttagc
ctcaacatcc atcctgtgga ggaggatgat 2220 attgcaatgt atttctgtca
gcaaagtagg aaggttccat ggacgttcgg tggaggcacc 2280
aagctggaaa tcaaacgtta atctaga 2307 <210> SEQ ID NO 223
<211> LENGTH: 762 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: n2H7sssIgG1-H4-2e12HL (AA) <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(20)
<223> OTHER INFORMATION: Leader <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(21)..(126) <223> OTHER INFORMATION: VL <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(509) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (510)..(630) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (631)..(650) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (651)..(762) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 223 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Asn Ser Gln Val Gln 500 505 510 Leu Lys Glu Ser Gly Pro Gly Leu Val
Ala Pro Ser Gln Ser Leu Ser 515 520 525 Ile Thr Cys Thr Val Ser Gly
Phe Ser Leu Thr Gly Tyr Gly Val Asn 530 535 540 Trp Val Arg Gln Pro
Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 545 550 555 560 Trp Gly
Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu 565 570 575
Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 580
585 590 Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp
Gly 595 600 605 Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly
Gln Gly Thr 610 615 620 Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser
Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Asp Ile Val Leu Thr Gln 645 650 655 Ser Pro Ala Ser Leu Ala
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser 660 665 670 Cys Arg Ala Ser
Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln 675 680 685 Trp Tyr
Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala 690 695 700
Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly 705
710 715 720 Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu
Asp Asp 725 730 735 Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val
Pro Trp Thr Phe 740 745 750 Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg
755 760 <210> SEQ ID NO 224 <211> LENGTH: 2313
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H5-2e12HL (DNA) <400> SEQUENCE: 224 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660 tcctccagca cagcctacat
gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 ttctgtgcaa
gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780
gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca
840 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
cccaaaaccc 900 aaggacaccc tcatgatctc ccggacccct gaggtcacat
gcgtggtggt ggacgtgagc 960 cacgaagacc ctgaggtcaa gttcaactgg
tacgtggacg gcgtggaggt gcataatgcc 1020 aagacaaagc cgcgggagga
gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 gtcctgcacc
aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140
ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag
1200 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
cctgacctgc 1260 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt
gggagagcaa tgggcagccg 1320 gagaacaact acaagaccac gcctcccgtg
ctggactccg acggctcctt cttcctctac 1380 agcaagctca ccgtggacaa
gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440
atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag
1500 aattatggtg gcggtggctc gggcggtggt ggatctggga attctcaggt
gcagctgaag 1560 gagtcaggac ctggcctggt ggcgccctca cagagcctgt
ccatcacatg caccgtctca 1620 gggttctcat taaccggcta tggtgtaaac
tgggttcgcc agcctccagg aaagggtctg 1680 gagtggctgg gaatgatatg
gggtgatgga agcacagact ataattcagc tctcaaatcc 1740 agactatcga
tcaccaagga caactccaag agccaagttt tcttaaaaat gaacagtctg 1800
caaactgatg acacagccag atactactgt gctcgagatg gttatagtaa ctttcattac
1860 tatgttatgg actactgggg tcaaggaacc tcagtcaccg tctcctctgg
gggtggaggc 1920 tctggtggcg gtggatccgg cggaggtggg tcgggtggcg
gcggatctga cattgtgctc 1980 acccaatctc cagcttcttt ggctgtgtct
ctaggtcaga gagccaccat ctcctgcaga 2040 gccagtgaaa gtgttgaata
ttatgtcaca agtttaatgc agtggtacca acagaaacca 2100 ggacagccac
ccaaactcct catctctgct gctagcaacg tagaatctgg ggtccctgcc 2160
aggtttagtg gcagtgggtc tgggacagac tttagcctca acatccatcc tgtggaggag
2220 gatgatattg caatgtattt ctgtcagcaa agtaggaagg ttccatggac
gttcggtgga 2280 ggcaccaagc tggaaatcaa acgttaatct aga 2313
<210> SEQ ID NO 225 <211> LENGTH: 764 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: n2H7sssIgG1-H5-2e12HL (AA)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(20) <223> OTHER INFORMATION: Leader
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (21)..(126) <223> OTHER INFORMATION: VL <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(127)..(142) <223> OTHER INFORMATION: Linker <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(511) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (512)..(632) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (633)..(652) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (653)..(764) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 225 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly Asn Ser Gln 500 505 510 Val Gln Leu Lys Glu Ser Gly Pro Gly
Leu Val Ala Pro Ser Gln Ser 515 520 525 Leu Ser Ile Thr Cys Thr Val
Ser Gly Phe Ser Leu Thr Gly Tyr Gly 530 535 540 Val Asn Trp Val Arg
Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly 545 550 555 560 Met Ile
Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser 565 570 575
Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 580
585 590 Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala
Arg 595 600 605 Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr
Trp Gly Gln 610 615 620 Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly
Gly Ser Gly Gly Gly 625 630 635 640 Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Asp Ile Val Leu 645 650 655 Thr Gln Ser Pro Ala Ser
Leu Ala Val Ser Leu Gly Gln Arg Ala Thr 660 665 670 Ile Ser Cys Arg
Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu 675 680 685 Met Gln
Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 690 695 700
Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly 705
710 715 720 Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val
Glu Glu 725 730 735 Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg
Lys Val Pro Trp 740 745 750 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
Lys Arg 755 760 <210> SEQ ID NO 226 <211> LENGTH: 2322
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H6-2e12HL (DNA) <400> SEQUENCE: 226 aagcttgccg
ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60
gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg
120 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca
ctggtaccag 180 cagaagccag gatcctcccc caaaccctgg atttatgccc
catccaacct ggcttctgga 240 gtccctgctc gcttcagtgg cagtgggtct
gggacctctt actctctcac aatcagcaga 300 gtggaggctg aagatgctgc
cacttattac tgccagcagt ggagttttaa cccacccacg 360 ttcggtgctg
ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420
ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct
480 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag
ttacaatatg 540 cactgggtaa agcagacacc tagacagggc ctggaatgga
ttggagctat ttatccagga 600 aatggtgata cttcctacaa tcagaagttc
aagggcaagg ccacactgac tgtagacaaa 660
tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat
720 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt
ctggggcaca 780 gggaccacgg tcaccgtctc gagcgagccc aaatcttctg
acaaaactca cacatcccca 840 ccgagcccag cacctgaact cctgggggga
ccgtcagtct tcctcttccc cccaaaaccc 900 aaggacaccc tcatgatctc
ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960 cacgaagacc
ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020
aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc
1080 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
caacaaagcc 1140 ctcccagccc ccatcgagaa aacaatctcc aaagccaaag
ggcagccccg agaaccacag 1200 gtgtacaccc tgcccccatc ccgggatgag
ctgaccaaga accaggtcag cctgacctgc 1260 ctggtcaaag gcttctatcc
cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320 gagaacaact
acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 1380
agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg
1440 atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc
tccgggtaag 1500 ggtggcggtg gctcgggcgg tggtggatct gggggaggag
gcagcgggaa ttctcaggtg 1560 cagctgaagg agtcaggacc tggcctggtg
gcgccctcac agagcctgtc catcacatgc 1620 accgtctcag ggttctcatt
aaccggctat ggtgtaaact gggttcgcca gcctccagga 1680 aagggtctgg
agtggctggg aatgatatgg ggtgatggaa gcacagacta taattcagct 1740
ctcaaatcca gactatcgat caccaaggac aactccaaga gccaagtttt cttaaaaatg
1800 aacagtctgc aaactgatga cacagccaga tactactgtg ctcgagatgg
ttatagtaac 1860 tttcattact atgttatgga ctactggggt caaggaacct
cagtcaccgt ctcctctggg 1920 ggtggaggct ctggtggcgg tggatccggc
ggaggtgggt cgggtggcgg cggatctgac 1980 attgtgctca cccaatctcc
agcttctttg gctgtgtctc taggtcagag agccaccatc 2040 tcctgcagag
ccagtgaaag tgttgaatat tatgtcacaa gtttaatgca gtggtaccaa 2100
cagaaaccag gacagccacc caaactcctc atctctgctg ctagcaacgt agaatctggg
2160 gtccctgcca ggtttagtgg cagtgggtct gggacagact ttagcctcaa
catccatcct 2220 gtggaggagg atgatattgc aatgtatttc tgtcagcaaa
gtaggaaggt tccatggacg 2280 ttcggtggag gcaccaagct ggaaatcaaa
cgttaatcta ga 2322 <210> SEQ ID NO 227 <211> LENGTH:
767 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION:
n2H7sssIgG1-H6-2e12HL (AA) <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(20) <223>
OTHER INFORMATION: Leader <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (21)..(126)
<223> OTHER INFORMATION: VL <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (127)..(142)
<223> OTHER INFORMATION: Linker <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(143)..(264) <223> OTHER INFORMATION: VH <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(265)..(279) <223> OTHER INFORMATION: Hinge <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(497)..(514) <223> OTHER INFORMATION: EFD-BD2 Linker
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (515)..(635) <223> OTHER INFORMATION: VH2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (636)..(655) <223> OTHER INFORMATION: Linker2
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (656)..(767) <223> OTHER INFORMATION: VL2
<400> SEQUENCE: 227 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu
Leu Leu Leu Trp Leu Pro 1 5 10 15 Asp Thr Thr Gly Gln Ile Val Leu
Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 Ala Ser Pro Gly Glu Lys
Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 Val Ser Tyr Met
His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 Pro Trp
Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80
Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85
90 95 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser
Phe 100 105 110 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
Lys Asp Gly 115 120 125 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ala Ser Gln Ala 130 135 140 Tyr Leu Gln Gln Ser Gly Ala Glu Leu
Val Arg Pro Gly Ala Ser Val 145 150 155 160 Lys Met Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 His Trp Val Lys
Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205
Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210
215 220 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
Arg 225 230 235 240 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp
Val Trp Gly Thr 245 250 255 Gly Thr Thr Val Thr Val Ser Ser Glu Pro
Lys Ser Ser Asp Lys Thr 260 265 270 His Thr Ser Pro Pro Ser Pro Ala
Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320 Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330
335 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
340 345 350 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
Glu Tyr 355 360 365 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile Glu Lys Thr 370 375 380 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val Tyr Thr Leu 385 390 395 400 Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455
460 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
465 470 475 480 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
Pro Gly Lys 485 490 495 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
Gly Gly Gly Ser Gly 500 505 510 Asn Ser Gln Val Gln Leu Lys Glu Ser
Gly Pro Gly Leu Val Ala Pro 515 520 525 Ser Gln Ser Leu Ser Ile Thr
Cys Thr Val Ser Gly Phe Ser Leu Thr 530 535 540 Gly Tyr Gly Val Asn
Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 545 550 555 560 Trp Leu
Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 565 570 575
Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val 580
585 590 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr
Tyr 595 600 605 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val
Met Asp Tyr 610 615 620 Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser
Gly Gly Gly Gly Ser 625 630 635 640 Gly Gly Gly Gly Ser Gly Gly Gly
Gly Ser Gly Gly Gly Gly Ser Asp 645 650 655 Ile Val Leu Thr Gln Ser
Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 660 665 670 Arg Ala Thr Ile
Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 675 680 685 Thr Ser
Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 690 695 700
Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg 705
710 715 720 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile
His Pro 725 730 735 Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln
Gln Ser Arg Lys 740 745 750 Val Pro Trp Thr Phe Gly Gly Gly Thr Lys
Leu Glu Ile Lys Arg 755 760 765 <210> SEQ ID NO 228
<211> LENGTH: 2337 <212> TYPE: DNA
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7sssIgG1-H7-G281 HL (DNA)
<400> SEQUENCE: 228 aagcttgccg ccatggattt tcaagtgcag
attttcagct tcctgctaat cagtgcttca 60 gtcataatgt ccagaggaca
aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 ccaggggaga
aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180
taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct
240 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc
tctcacaatc 300 agcagagtgg aggctgaaga tgctgccact tattactgcc
agcagtggag ttttaaccca 360 cccacgttcg gtgctgggac caagctggag
ctgaaagatg gcggtggctc gggcggtggt 420 ggatctggag gaggtgggag
ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 aggcctgggg
cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540
aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat
600 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac
actgactgta 660 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc
tgacatctga agactctgcg 720 gtctatttct gtgcaagagt ggtgtactat
agtaactctt actggtactt cgatgtctgg 780 ggcacaggga ccacggtcac
cgtctctgat caggagccca aatcttgtga caaaactcac 840 acatccccac
cgtgctcagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900
ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg
960 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
cgtggaggtg 1020 cataatgcca agacaaagcc gcgggaggag cagtacaaca
gcacgtaccg tgtggtcagc 1080 gtcctcaccg tcctgcacca ggactggctg
aatggcaagg agtacaagtg caaggtctcc 1140 aacaaagccc tcccagcccc
catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 gaaccacagg
tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260
ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat
1320 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
cggctccttc 1380 ttcctctaca gcaagctcac cgtggacaag agcaggtggc
agcaggggaa cgtcttctca 1440 tgctccgtga tgcatgaggc tctgcacaac
cactacacgc agaagagcct ctccctgtct 1500 ccgggtaaga attatggtgg
cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560 gggaattctc
aggtgcagct gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 1620
ctgtccatca catgcaccgt ctcagggttc tcattaaccg gctatggtgt aaactgggtt
1680 cgccagcctc caggaaaggg tctggagtgg ctgggaatga tatggggtga
tggaagcaca 1740 gactataatt cagctctcaa atccagacta tcgatcacca
aggacaactc caagagccaa 1800 gttttcttaa aaatgaacag tctgcaaact
gatgacacag ccagatacta ctgtgctcga 1860 gatggttata gtaactttca
ttactatgtt atggactact ggggtcaagg aacctcagtc 1920 accgtctcct
ctgggggtgg aggctctggt ggcggtggat ccggcggagg tgggtcgggt 1980
ggcggcggat ctgacattgt gctcacccaa tctccagctt ctttggctgt gtctctaggt
2040 cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt
cacaagttta 2100 atgcagtggt accaacagaa accaggacag ccacccaaac
tcctcatctc tgctgctagc 2160 aacgtagaat ctggggtccc tgccaggttt
agtggcagtg ggtctgggac agactttagc 2220 ctcaacatcc atcctgtgga
ggaggatgat attgcaatgt atttctgtca gcaaagtagg 2280 aaggttccat
ggacgttcgg tggaggcacc aagctggaaa tcaaacgtta atctaga 2337
<210> SEQ ID NO 229 <211> LENGTH: 772 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: 2H7cscIgG1-STD1-2e12HL (w/2E12
leader) (AA) <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (1)..(22) <223> OTHER
INFORMATION: Leader <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (23)..(128) <223> OTHER
INFORMATION: VL <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (129)..(144) <223> OTHER
INFORMATION: Linker <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (145)..(265) <223> OTHER
INFORMATION: VH <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (268)..(282) <223> OTHER
INFORMATION: Hinge <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (500)..(519) <223> OTHER
INFORMATION: EFD-BD2 Linker <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (520)..(640)
<223> OTHER INFORMATION: VH2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (641)..(660)
<223> OTHER INFORMATION: Linker2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(661)..(772) <223> OTHER INFORMATION: VL2 <400>
SEQUENCE: 229 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile
Ser Ala Ser 1 5 10 15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser
Gln Ser Pro Ala Ile 20 25 30 Leu Ser Ala Ser Pro Gly Glu Lys Val
Thr Met Thr Cys Arg Ala Ser 35 40 45 Ser Ser Val Ser Tyr Met His
Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 Pro Lys Pro Trp Ile
Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 Ser
Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105
110 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
115 120 125 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Ser 130 135 140 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val
Arg Pro Gly Ala 145 150 155 160 Ser Val Lys Met Ser Cys Lys Ala Ser
Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 Asn Met His Trp Val Lys Gln
Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 Gly Ala Ile Tyr Pro
Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 Lys Gly Lys
Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 Met
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230
235 240 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 245 250 255 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro
Lys Ser Cys 260 265 270 Asp Lys Thr His Thr Ser Pro Pro Cys Ser Ala
Pro Glu Leu Leu Gly 275 280 285 Gly Pro Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 290 295 300 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 Glu Asp Pro Glu
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 His Asn
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355
360 365 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile 370 375 380 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 385 390 395 400 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
Thr Lys Asn Gln Val Ser 405 410 415 Leu Thr Cys Leu Val Lys Gly Phe
Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 Trp Glu Ser Asn Gly Gln
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475
480 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
485 490 495 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser Gly 500 505 510 Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys
Glu Ser Gly Pro 515 520 525 Gly Leu Val Ala Pro Ser Gln Ser Leu Ser
Ile Thr Cys Thr Val Ser 530 535 540 Gly Phe Ser Leu Thr Gly Tyr Gly
Val Asn Trp Val Arg Gln Pro Pro 545 550 555 560 Gly Lys Gly Leu Glu
Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 565 570 575 Asp Tyr Asn
Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 580 585 590 Ser
Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 595 600
605 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr
610 615 620 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 625 630 635 640
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 645
650 655 Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu
Ala 660 665 670 Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala
Ser Glu Ser 675 680 685 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp
Tyr Gln Gln Lys Pro 690 695 700 Gly Gln Pro Pro Lys Leu Leu Ile Ser
Ala Ala Ser Asn Val Glu Ser 705 710 715 720 Gly Val Pro Ala Arg Phe
Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 725 730 735 Leu Asn Ile His
Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 740 745 750 Gln Gln
Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 755 760 765
Glu Ile Lys Arg 770 <210> SEQ ID NO 230 <211> LENGTH:
45 <212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: scs(s) -hIgG1
(DNA) <400> SEQUENCE: 230 gagcccaaat cttctgacaa aactcacaca
tctccaccga gctca 45 <210> SEQ ID NO 231 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: scs(s)
-hIgG1 (AA) <400> SEQUENCE: 231 Glu Pro Lys Ser Ser Asp Lys
Thr His Thr Ser Pro Pro Ser Ser 1 5 10 15 <210> SEQ ID NO 232
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: ccc(s) (DNA) <400>
SEQUENCE: 232 gagcccaaat cttgtgacaa aactcacaca tctccaccgt gctca 45
<210> SEQ ID NO 233 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: ccc(s) (AA) <400>
SEQUENCE: 233 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro
Cys Ser 1 5 10 15 <210> SEQ ID NO 234 <211> LENGTH: 24
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H8 (PN) <400> SEQUENCE: 234 gggtctccac
cttctccgaa ttct 24 <210> SEQ ID NO 235 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <400> SEQUENCE: 235 Gly Ser Pro Pro Ser Pro Asn Ser 1
5 <210> SEQ ID NO 236 <211> LENGTH: 21 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H9 (PN) <400> SEQUENCE: 236 tctccacctt ctccgaattc t 21
<210> SEQ ID NO 237 <211> LENGTH: 7 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H9 (AA) <400>
SEQUENCE: 237 Ser Pro Pro Ser Pro Asn Ser 1 5 <210> SEQ ID NO
238 <400> SEQUENCE: 238 000 <210> SEQ ID NO 239
<400> SEQUENCE: 239 000 <210> SEQ ID NO 240 <211>
LENGTH: 51 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker polynucleotide <220> FEATURE:
<223> OTHER INFORMATION: Linker H11 (PN) <400>
SEQUENCE: 240 gagcccacat ctaccgacaa aactcacaca tctccaccca
gcccgaattc t 51 <210> SEQ ID NO 241 <211> LENGTH: 17
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H11 (AA) <400> SEQUENCE: 241 Glu Pro Thr Ser Thr Asp Lys Thr
His Thr Ser Pro Pro Ser Pro Asn 1 5 10 15 Ser <210> SEQ ID NO
242 <211> LENGTH: 51 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H12 (PN)
<400> SEQUENCE: 242 gagcccacat ctaccgacaa aactcacaca
tctccaccca gcccgaattc t 51 <210> SEQ ID NO 243 <211>
LENGTH: 17 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H12 (AA) <400> SEQUENCE: 243 Glu Pro Thr
Ser Thr Asp Lys Thr His Thr Cys Pro Pro Cys Pro Asn 1 5 10 15 Ser
<210> SEQ ID NO 244 <211> LENGTH: 51 <212> TYPE:
DNA <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H13 (PN) <400> SEQUENCE: 244 gagcccacat ctaccgacaa
aactcacaca tctccaccca gcccgaattc t 51 <210> SEQ ID NO 245
<211> LENGTH: 17 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H13 (AA) <400>
SEQUENCE: 245 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro
Cys Pro Asn 1 5 10 15 Ser <210> SEQ ID NO 246 <211>
LENGTH: 36 <212> TYPE: DNA <213> ORGANISM: Artificial
sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H15 (PN) <400> SEQUENCE: 246 ggcggtggtg
gctcctgtcc accttgtccg aattct 36 <210> SEQ ID NO 247
<211> LENGTH: 12 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H15 (AA) <400>
SEQUENCE: 247 Gly Gly Gly Gly Ser Cys Pro Pro Cys Pro Asn Ser 1 5
10 <210> SEQ ID NO 248 <211> LENGTH: 45 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H16 (PN) <400> SEQUENCE: 248 ctgtctgtga aagctgactt
cctcactcca tccatcggga attct 45 <210> SEQ ID NO 249
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H16 (AA) <400>
SEQUENCE: 249 Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly
Asn Ser 1 5 10 15 <210> SEQ ID NO 250 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H17 (PN) <400> SEQUENCE: 250 ctgtctgtga
aagctgactt cctcactcca tccatctcct gtccaccttg cccgaattct 60
<210> SEQ ID NO 251 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H17 (AA) <400>
SEQUENCE: 251 Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Ser
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 252
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H18 (PN) <400>
SEQUENCE: 252 ctgtctgtgc tcgctaactt cagtcagcca gagatcggga attct 45
<210> SEQ ID NO 253 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H18 (AA) <400>
SEQUENCE: 253 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly
Asn Ser 1 5 10 15 <210> SEQ ID NO 254 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H19 (PN) <400> SEQUENCE: 254 ctgtctgtgc
tcgctaactt cagtcagcca gagatctcct gtccaccttg cccgaattct 60
<210> SEQ ID NO 255 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H19 (AA) <400>
SEQUENCE: 255 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Ser
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 256
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H20 (PN) <400>
SEQUENCE: 256 ctgaaaatcc aggagagggt cagtaagcca aagatctcga attct 45
<210> SEQ ID NO 257 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H20 (AA) <400>
SEQUENCE: 257 Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser
Asn Ser 1 5 10 15 <210> SEQ ID NO 258 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polypeptide <220> FEATURE: <223> OTHER
INFORMATION: Linker H21 (PN) <400> SEQUENCE: 258 ctgaaaatcc
aggagagggt cagtaagcca aagatctcct gtccaccttg cccgaattct 60
<210> SEQ ID NO 259 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H21 (AA) <400>
SEQUENCE: 259 Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 260
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polypeptide <220>
FEATURE: <223> OTHER INFORMATION: Linker H22 (PN) <400>
SEQUENCE: 260 ctggatgtga gtgagaggcc ttttcctcca cacatccaga attct 45
<210> SEQ ID NO 261 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H22 (AA) <400>
SEQUENCE: 261 Leu Asp Val Ser Glu Arg Pro Phe Pro Pro His Ile Gln
Asn Ser 1 5 10 15 <210> SEQ ID NO 262 <211> LENGTH: 63
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H23 (PN) <400> SEQUENCE: 262 ctggatgtga
gtgagaggcc ttttcctcca cacatccagt cctgtccacc ttgcccgaat 60 tct
63
<210> SEQ ID NO 263 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H23 (AA) <400>
SEQUENCE: 263 Leu Asp Val Ser Glu Arg Pro Phe Pro Pro His Ile Gln
Ser Cys Pro 1 5 10 15 Pro Cys Pro Asn Ser 20 <210> SEQ ID NO
264 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H24
(DNA) <400> SEQUENCE: 264 cgggaacagc tggcagaggt cactttgagc
ttgaaagcga attct 45 <210> SEQ ID NO 265 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H24 (AA) <400> SEQUENCE: 265 Arg Glu Gln Leu Ala Glu Val Thr
Leu Ser Leu Lys Ala Asn Ser 1 5 10 15 <210> SEQ ID NO 266
<211> LENGTH: 60 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H25 (PN) <400>
SEQUENCE: 266 cgggaacagc tggcagaggt cactttgagc gtgaaagctt
gtccaccctg cccgaattct 60 <210> SEQ ID NO 267 <211>
LENGTH: 20 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H25 (AA) <400> SEQUENCE: 267 Arg Glu Gln
Leu Ala Glu Val Thr Leu Ser Leu Lys Ala Cys Pro Pro 1 5 10 15 Cys
Pro Asn Ser 20 <210> SEQ ID NO 268 <211> LENGTH: 45
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H26 (PN) <400> SEQUENCE: 268 cggattcacc
agatgaactc cgagttgagc gtgctcgcga attct 45 <210> SEQ ID NO 269
<211> LENGTH: 15 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H26 (AA) <400>
SEQUENCE: 269 Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala
Asn Ser 1 5 10 15 <210> SEQ ID NO 270 <211> LENGTH: 60
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H27 (PN) <400> SEQUENCE: 270 cggattcacc
agatgaactc cgagttgagc gtgctcgctt gtccaccctg cccgaattct 60
<210> SEQ ID NO 271 <211> LENGTH: 20 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H27 (AA) <400>
SEQUENCE: 271 Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala
Cys Pro Pro 1 5 10 15 Cys Pro Asn Ser 20 <210> SEQ ID NO 272
<211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H28 (PN) <400>
SEQUENCE: 272 gataccaaag ggaagaacgt cctcgagaag atcttctcga attct 45
<210> SEQ ID NO 273 <211> LENGTH: 15 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H28 (AA) <400>
SEQUENCE: 273 Asp Thr Lys Gly Lys Asn Val Leu Glu Lys Ile Phe Ser
Asn Ser 1 5 10 15 <210> SEQ ID NO 274 <211> LENGTH: 63
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H29 (PN) <400> SEQUENCE: 274 gataccaaag
ggaagaacgt cctcgagaag atcttcgact cctgtccacc ttgcccgaat 60 tct 63
<210> SEQ ID NO 275 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H29 (AA) <400>
SEQUENCE: 275 Asp Thr Lys Gly Lys Asn Val Leu Glu Lys Ile Phe Asp
Ser Cys Pro 1 5 10 15 Pro Cys Pro Asn Ser 20 <210> SEQ ID NO
276 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H30 (PN)
<400> SEQUENCE: 276 ctgccacctg agacacagga gagtcaagaa
gtcaccctga attct 45 <210> SEQ ID NO 277 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H30 (AA) <400> SEQUENCE: 277 Leu Pro Pro Glu Thr Gln Glu Ser
Gln Glu Val Thr Leu Asn Ser 1 5 10 15 <210> SEQ ID NO 278
<211> LENGTH: 63 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H31 (PN) <400>
SEQUENCE: 278 ctgccacctg agacacagga gagtcaagaa gtcaccctgt
cctgtccacc ttgcccgaat 60 tct 63 <210> SEQ ID NO 279
<211> LENGTH: 21 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H31 (AA) <400>
SEQUENCE: 279 Leu Pro Pro Glu Thr Gln Glu Ser Gln Glu Val Thr Leu
Ser Cys Pro 1 5 10 15 Pro Cys Pro Asn Ser 20 <210> SEQ ID NO
280 <211> LENGTH: 45 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H32 (PN)
<400> SEQUENCE: 280 cggattcacc tgaacgtgtc cgagaggccc
tttcctccga attct 45 <210> SEQ ID NO 281 <211> LENGTH:
15 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H32 (AA) <400> SEQUENCE: 281 Arg Ile His Leu Asn Val Ser Glu
Arg Pro Phe Pro Pro Asn Ser 1 5 10 15 <210> SEQ ID NO 282
<211> LENGTH: 60 <212> TYPE: DNA <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker polynucleotide <220>
FEATURE: <223> OTHER INFORMATION: Linker H33 (PN) <400>
SEQUENCE: 282 cggattcacc tgaacgtgtc cgagaggccc tttcctccct
gtccaccctg cccgaattct 60 <210> SEQ ID NO 283 <211>
LENGTH: 20 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H33 (AA) <400> SEQUENCE: 283 Arg Ile His
Leu Asn Val Ser Glu Arg Pro Phe Pro Pro Cys Pro Pro 1 5 10 15 Cys
Pro Asn Ser 20 <210> SEQ ID NO 284 <400> SEQUENCE: 284
000 <210> SEQ ID NO 285 <400> SEQUENCE: 285 000
<210> SEQ ID NO 286 <400> SEQUENCE: 286 000 <210>
SEQ ID NO 287 <400> SEQUENCE: 287 000 <210> SEQ ID NO
288 <211> LENGTH: 39 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H36 (PN) <400>
SEQUENCE: 288 gggtgtccac cttgtccagg cggtggtgga tcgaattct 39
<210> SEQ ID NO 289 <211> LENGTH: 13 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H36 (AA) <400>
SEQUENCE: 289 Gly Cys Pro Pro Cys Pro Gly Gly Gly Gly Ser Asn Ser 1
5 10 <210> SEQ ID NO 290 <400> SEQUENCE: 290 000
<210> SEQ ID NO 291 <400> SEQUENCE: 291 000 <210>
SEQ ID NO 292 <400> SEQUENCE: 292 000 <210> SEQ ID NO
293 <400> SEQUENCE: 293 000 <210> SEQ ID NO 294
<400> SEQUENCE: 294 000 <210> SEQ ID NO 295 <400>
SEQUENCE: 295 000 <210> SEQ ID NO 296 <211> LENGTH: 27
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H40 (PN) <400> SEQUENCE: 296 ggatgtccac
cttgtcccgc gaattct 27 <210> SEQ ID NO 297 <211> LENGTH:
9 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H40 (AA) <400> SEQUENCE: 297 Gly Cys Pro Pro Cys Pro Ala Asn
Ser 1 5 <210> SEQ ID NO 298 <400> SEQUENCE: 298 000
<210> SEQ ID NO 299 <211> LENGTH: 9 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: Linker H41 <400> SEQUENCE: 299 Gly Cys Pro Pro
Cys Pro Ala Asn Ser 1 5 <210> SEQ ID NO 300 <400>
SEQUENCE: 300 000 <210> SEQ ID NO 301 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H42(AA) <400> SEQUENCE: 301 Gly Cys Pro Pro Cys Pro Asn Ser 1
5
<210> SEQ ID NO 302 <400> SEQUENCE: 302 000 <210>
SEQ ID NO 303 <400> SEQUENCE: 303 000 <210> SEQ ID NO
304 <211> LENGTH: 36 <212> TYPE: DNA <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker polynucleotide
<220> FEATURE: <223> OTHER INFORMATION: Linker H44 (PN)
<400> SEQUENCE: 304 ggaggagcta gttgtccacc ttgtcccggg aattct
36 <210> SEQ ID NO 305 <211> LENGTH: 12 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H44 (AA)
<400> SEQUENCE: 305 Gly Gly Ala Ser Cys Pro Pro Cys Pro Gly
Asn Ser 1 5 10 <210> SEQ ID NO 306 <211> LENGTH: 36
<212> TYPE: DNA <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker polynucleotide <220> FEATURE: <223> OTHER
INFORMATION: Linker H45 (PN) <400> SEQUENCE: 306 ggaggagcca
gttgtccacc ttgtgccggg aattct 36 <210> SEQ ID NO 307
<211> LENGTH: 12 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H45 (AA) <400>
SEQUENCE: 307 Gly Gly Ala Ser Cys Pro Pro Cys Ala Gly Asn Ser 1 5
10 <210> SEQ ID NO 308 <211> LENGTH: 33 <212>
TYPE: DNA <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
polynucleotide <220> FEATURE: <223> OTHER INFORMATION:
Linker H46 <400> SEQUENCE: 308 ggaggagcca gttgtccacc
ttgtgcgaat tct 33 <210> SEQ ID NO 309 <211> LENGTH: 11
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H46 <400> SEQUENCE: 309 Gly Gly Ala Ser Cys Pro Pro Cys Ala
Asn Ser 1 5 10 <210> SEQ ID NO 310 <211> LENGTH: 15
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H47 <400> SEQUENCE: 310 Leu Ser Val Lys Ala Asp Phe Leu Thr
Pro Ser Ile Gly Asn Ser 1 5 10 15 <210> SEQ ID NO 311
<211> LENGTH: 12 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H48 <400> SEQUENCE: 311
Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly Asn Ser 1 5 10 <210>
SEQ ID NO 312 <400> SEQUENCE: 312 000 <210> SEQ ID NO
313 <211> LENGTH: 15 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H50 <400> SEQUENCE: 313
Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly Asn Ser 1 5 10
15 <210> SEQ ID NO 314 <211> LENGTH: 15 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H51
<400> SEQUENCE: 314 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro
Glu Ile Gly Asn Ser 1 5 10 15 <210> SEQ ID NO 315 <211>
LENGTH: 11 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthetic peptide <220> FEATURE: <223> OTHER
INFORMATION: Scorpion region linker <400> SEQUENCE: 315 Ser
Gln Pro Glu Ile Val Pro Ile Ser Asn Ser 1 5 10 <210> SEQ ID
NO 316 <211> LENGTH: 16 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H53 <400> SEQUENCE: 316
Ser Gln Pro Glu Ile Val Pro Ile Ser Cys Pro Pro Cys Pro Asn Ser 1 5
10 15 <210> SEQ ID NO 317 <211> LENGTH: 19 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H54
<400> SEQUENCE: 317 Ser Val Leu Ala Asn Phe Ser Gln Pro Glu
Ile Ser Cys Pro Pro Cys 1 5 10 15 Pro Asn Ser <210> SEQ ID NO
318 <211> LENGTH: 15 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H55 <400> SEQUENCE: 318
Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn Ser 1 5 10
15 <210> SEQ ID NO 319 <211> LENGTH: 12 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H56
<400> SEQUENCE: 319 Gln Met Asn Ser Glu Leu Ser Val Leu Ala
Asn Ser 1 5 10 <210> SEQ ID NO 320 <211> LENGTH: 10
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H57
<400> SEQUENCE: 320 Val Ser Glu Arg Pro Phe Pro Pro Asn Ser 1
5 10 <210> SEQ ID NO 321 <211> LENGTH: 15 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Scorpion region linker
<220> FEATURE: <223> OTHER INFORMATION: Linker H58
<400> SEQUENCE: 321 Lys Pro Phe Phe Thr Cys Gly Ser Ala Asp
Thr Cys Pro Asn Ser 1 5 10 15 <210> SEQ ID NO 322 <211>
LENGTH: 15 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H59 <400> SEQUENCE: 322 Lys Pro Phe Phe
Thr Cys Gly Ser Ala Asp Thr Cys Pro Asn Ser 1 5 10 15 <210>
SEQ ID NO 323 <211> LENGTH: 14 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H60 <400>
SEQUENCE: 323 Gln Tyr Asn Cys Pro Gly Gln Tyr Thr Phe Ser Met Asn
Ser 1 5 10 <210> SEQ ID NO 324 <211> LENGTH: 21
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H61 <400> SEQUENCE: 324 Glu Pro Ala Phe Thr Pro Gly Pro Asn
Ile Glu Leu Gln Lys Asp Ser 1 5 10 15 Asp Cys Pro Asn Ser 20
<210> SEQ ID NO 325 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H62 <400>
SEQUENCE: 325 Gln Arg His Asn Asn Ser Ser Leu Asn Thr Arg Thr Gln
Lys Ala Arg 1 5 10 15 His Cys Pro Asn Ser 20 <210> SEQ ID NO
326 <211> LENGTH: 19 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H63 <400> SEQUENCE: 326
Asn Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr 1 5
10 15 Cys Asn Ser <210> SEQ ID NO 327 <211> LENGTH: 21
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <400> SEQUENCE: 327 Gln Arg His Asn Asn Ser Ser Leu
Asn Thr Arg Thr Gln Lys Ala Arg 1 5 10 15 His Cys Pro Asn Ser 20
<210> SEQ ID NO 328 <211> LENGTH: 21 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Scorpion region linker <220>
FEATURE: <223> OTHER INFORMATION: Linker H65 <400>
SEQUENCE: 328 Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln
Lys Asp Ser 1 5 10 15 Asp Cys Pro Asn Ser 20 <210> SEQ ID NO
329 <211> LENGTH: 21 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion region linker <220> FEATURE:
<223> OTHER INFORMATION: Linker H66 <400> SEQUENCE: 329
Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5
10 15 Asp Cys Pro Asn Ser 20 <210> SEQ ID NO 330 <211>
LENGTH: 21 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Scorpion region linker <220> FEATURE: <223> OTHER
INFORMATION: Linker H67 <400> SEQUENCE: 330 Glu Pro Ala Phe
Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5 10 15 Asp Cys
Pro Asn Ser 20 <210> SEQ ID NO 331 <211> LENGTH: 118
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion region
linker <220> FEATURE: <223> OTHER INFORMATION: Linker
H68 <400> SEQUENCE: 331 Arg Thr Arg Tyr Leu Gln Val Ser Gln
Gln Leu Gln Gln Thr Asn Arg 1 5 10 15 Val Leu Glu Val Thr Asn Ser
Ser Leu Arg Gln Gln Leu Arg Leu Lys 20 25 30 Ile Thr Gln Leu Gly
Gln Ser Ala Glu Asp Leu Gln Gly Ser Arg Arg 35 40 45 Glu Leu Ala
Gln Ser Gln Glu Ala Leu Gln Val Glu Gln Arg Ala His 50 55 60 Gln
Ala Ala Glu Gly Gln Leu Gln Ala Cys Gln Ala Asp Arg Gln Lys 65 70
75 80 Thr Lys Glu Thr Leu Gln Ser Glu Glu Gln Gln Arg Arg Ala Leu
Glu 85 90 95 Gln Lys Leu Ser Asn Met Glu Asn Arg Leu Lys Pro Phe
Phe Thr Cys 100 105 110 Gly Ser Ala Asp Thr Cys 115 <210> SEQ
ID NO 332 <211> LENGTH: 10 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: anti-CD-20 VL CDR1 <400> SEQUENCE: 332 Arg
Ala Ser Ser Ser Val Ser Tyr Ile His 1 5 10 <210> SEQ ID NO
333 <211> LENGTH: 10 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: anti-CD-20 VL CDR1 <400> SEQUENCE: 333 Arg
Ala Ser Ser Ser Val Ser Tyr Ile Val 1 5 10 <210> SEQ ID NO
334 <211> LENGTH: 9 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: anti-CD-20 VL CDR3 <400> SEQUENCE: 334 Gln
Gln Trp Ser Phe Asn Pro Pro Thr 1 5
<210> SEQ ID NO 335 <211> LENGTH: 9 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD-20 VL CDR3 <400>
SEQUENCE: 335 Gln Gln Tyr Ser Phe Asn Pro Pro Thr 1 5 <210>
SEQ ID NO 336 <211> LENGTH: 17 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD-20 VH CDR2 <400>
SEQUENCE: 336 Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln
Lys Phe Lys 1 5 10 15 Gly <210> SEQ ID NO 337 <211>
LENGTH: 17 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
anti-CD-20 VH CDR2 <400> SEQUENCE: 337 Ala Ile Tyr Pro Gly
Asn Gly Glu Thr Ser Tyr Asn Gln Lys Phe Lys 1 5 10 15 Gly
<210> SEQ ID NO 338 <211> LENGTH: 12 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD20 VH CDR3 <400>
SEQUENCE: 338 Ser Val Tyr Tyr Ser Asn Tyr Trp Tyr Phe Asp Leu 1 5
10 <210> SEQ ID NO 339 <211> LENGTH: 12 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: anti-CD20 VH CDR3
<400> SEQUENCE: 339 Ser Val Tyr Tyr Gly Gly Tyr Trp Tyr Phe
Asp Leu 1 5 10 <210> SEQ ID NO 340 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 340 Ser Tyr Tyr Ser Asn Ser Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 341 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 341 Ser Tyr Tyr Ser Gly Gly Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 342 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 342 Ser Tyr Lys Ser Asn Ser Tyr Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 343 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 343 Ser Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 344 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD-20 VH
CDR3 <400> SEQUENCE: 344 Ser Tyr Lys Ser Asn Ser Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 345 <211> LENGTH: 12
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: anti-CD20 VH
CDR3 <400> SEQUENCE: 345 Ser Tyr Lys Ser Gly Gly Asp Trp Tyr
Phe Asp Leu 1 5 10 <210> SEQ ID NO 346 <211> LENGTH: 8
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
core (2H7) sequence <400> SEQUENCE: 346 Gly Cys Pro Pro Cys
Pro Asn Ser 1 5 <210> SEQ ID NO 347 <211> LENGTH: 4
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Extension
sequence <400> SEQUENCE: 347 Ala Pro Glu Leu 1 <210>
SEQ ID NO 348 <211> LENGTH: 9 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthetic peptide <220>
FEATURE: <223> OTHER INFORMATION: Extension sequence
<400> SEQUENCE: 348 Ala Pro Glu Leu Gly Gly Gly Gly Ser 1 5
<210> SEQ ID NO 349 <211> LENGTH: 14 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Extension sequence <400>
SEQUENCE: 349 Ala Pro Glu Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly
Ser 1 5 10 <210> SEQ ID NO 350 <211> LENGTH: 19
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Extension
sequence <400> SEQUENCE: 350 Ala Pro Glu Leu Gly Gly Gly Gly
Ser Gly Gly Gly Gly Ser Gly Gly 1 5 10 15 Gly Gly Ser <210>
SEQ ID NO 351 <211> LENGTH: 12 <212> TYPE: PRT
<213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Extended scorpion linker <400>
SEQUENCE: 351 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu 1 5
10 <210> SEQ ID NO 352 <211> LENGTH: 17 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Extended scorpion linker
<400> SEQUENCE: 352 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro
Glu Leu Gly Gly Gly Gly 1 5 10 15 Ser <210> SEQ ID NO 353
<211> LENGTH: 22 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Extended scorpion linker
<400> SEQUENCE: 353 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro
Glu Leu Gly Gly Gly Gly 1 5 10 15 Ser Gly Gly Gly Gly Ser 20
<210> SEQ ID NO 354 <211> LENGTH: 27 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Extended scorpion linker <400>
SEQUENCE: 354 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu Gly
Gly Gly Gly 1 5 10 15 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
20 25 <210> SEQ ID NO 355 <400> SEQUENCE: 355 000
<210> SEQ ID NO 356 <400> SEQUENCE: 356 000 <210>
SEQ ID NO 357 <400> SEQUENCE: 357 000 <210> SEQ ID NO
358 <400> SEQUENCE: 358 000 <210> SEQ ID NO 359
<400> SEQUENCE: 359 000 <210> SEQ ID NO 360 <400>
SEQUENCE: 360 000 <210> SEQ ID NO 361 <400> SEQUENCE:
361 000 <210> SEQ ID NO 362 <400> SEQUENCE: 362 000
<210> SEQ ID NO 363 <400> SEQUENCE: 363 000 <210>
SEQ ID NO 364 <400> SEQUENCE: 364 000 <210> SEQ ID NO
365 <400> SEQUENCE: 365 000 <210> SEQ ID NO 366
<211> LENGTH: 10 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: partial CH3 sequence <400> SEQUENCE: 366 Gln Lys
Ser Leu Ser Leu Ser Pro Gly Lys 1 5 10 <210> SEQ ID NO 367
<211> LENGTH: 9 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Partial CH3 sequence <400> SEQUENCE: 367 Gln Lys
Ser Leu Ser Leu Ser Pro Gly 1 5 <210> SEQ ID NO 368
<211> LENGTH: 1 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Partial CH3 sequence <400> SEQUENCE: 368 Gln 1
<210> SEQ ID NO 369 <211> LENGTH: 3 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: Partial CH3 sequence <400>
SEQUENCE: 369 Gln Lys Ser 1 <210> SEQ ID NO 370 <211>
LENGTH: 6 <212> TYPE: PRT <213> ORGANISM: Artificial
sequence <220> FEATURE: <223> OTHER INFORMATION:
Partial CH3 sequence <400> SEQUENCE: 370 Gln Lys Ser Leu Ser
Leu 1 5 <210> SEQ ID NO 371 <211> LENGTH: 5 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: Partial CH3 sequence
<400> SEQUENCE: 371 Gln Lys Ser Leu Ser 1 5 <210> SEQ
ID NO 372 <211> LENGTH: 5 <212> TYPE: PRT <213>
ORGANISM: Artificial sequence <220> FEATURE: <223>
OTHER INFORMATION: Scorpion linker <400> SEQUENCE: 372 Gly
Cys Pro Pro Cys 1 5 <210> SEQ ID NO 373 <211> LENGTH:
12 <212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
<400> SEQUENCE: 373 Gln Tyr Asn Cys Pro Gly Gln Tyr Thr Phe
Ser Met 1 5 10 <210> SEQ ID NO 374 <211> LENGTH: 11
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
<400> SEQUENCE: 374 Pro Phe Phe Thr Cys Gly Ser Ala Asp Thr
Cys 1 5 10 <210> SEQ ID NO 375 <211> LENGTH: 18
<212> TYPE: PRT <213> ORGANISM: Artificial sequence
<220> FEATURE: <223> OTHER INFORMATION: Scorpion linker
<400> SEQUENCE: 375 Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile
Glu Leu Gln Lys Asp Ser 1 5 10 15 Asp Cys <210> SEQ ID NO 376
<211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker <400> SEQUENCE: 376 Gln Arg His
Asn Asn Ser Ser Leu Asn Thr Arg Thr Gln Lys Ala Arg 1 5 10 15 His
Cys <210> SEQ ID NO 377
<211> LENGTH: 17 <212> TYPE: PRT <213> ORGANISM:
Artificial sequence <220> FEATURE: <223> OTHER
INFORMATION: Scorpion linker <400> SEQUENCE: 377 Asn Ser Leu
Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr 1 5 10 15 Cys
<210> SEQ ID NO 378 <211> LENGTH: 10 <212> TYPE:
PRT <213> ORGANISM: Artificial sequence <220> FEATURE:
<223> OTHER INFORMATION: anti-CD20 VL CDR1 (TRU-015)
<400> SEQUENCE: 378 Arg Ala Ser Ser Ser Val Ser Tyr Met His 1
5 10 <210> SEQ ID NO 379 <211> LENGTH: 13 <212>
TYPE: PRT <213> ORGANISM: Artificial sequence <220>
FEATURE: <223> OTHER INFORMATION: anti-CD20 VH CDR3 (TRU-015)
<400> SEQUENCE: 379 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr
Phe Asp Val 1 5 10
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