U.S. patent application number 14/091723 was filed with the patent office on 2014-04-10 for antiviral drugs for treatment of arenvirus infection.
This patent application is currently assigned to SIGA TECHNOLOGIES, INC.. The applicant listed for this patent is SIGA TECHNOLOGIES, INC.. Invention is credited to SEAN M. AMBERG, TOVE C. BOLKEN, Dongcheng Dai, DENNIS E. HRUBY, TRAVIS K. WARREN.
Application Number | 20140100245 14/091723 |
Document ID | / |
Family ID | 41136068 |
Filed Date | 2014-04-10 |
United States Patent
Application |
20140100245 |
Kind Code |
A1 |
Dai; Dongcheng ; et
al. |
April 10, 2014 |
Antiviral Drugs for Treatment of Arenvirus Infection
Abstract
Compounds, methods and pharmaceutical compositions for treating
viral infections, by administering certain compounds in
therapeutically effective amounts are disclosed. Methods for
preparing the compounds and methods of using the compounds and
pharmaceutical compositions thereof are also disclosed. In
particular, the treatment and prophylaxis of viral infections such
as caused by the Arenavirus family is disclosed.
Inventors: |
Dai; Dongcheng; (CORVALLIS,
OR) ; HRUBY; DENNIS E.; (ALBANY, OR) ; BOLKEN;
TOVE C.; (KEIZER, OR) ; AMBERG; SEAN M.;
(CORVALLIS, OR) ; WARREN; TRAVIS K.; (MIDDLETOWN,
MD) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
SIGA TECHNOLOGIES, INC. |
CORVALLIS |
OR |
US |
|
|
Assignee: |
SIGA TECHNOLOGIES, INC.
CORVALLIS
OR
|
Family ID: |
41136068 |
Appl. No.: |
14/091723 |
Filed: |
November 27, 2013 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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12735392 |
Sep 29, 2010 |
8623347 |
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PCT/US2009/030919 |
Jan 14, 2009 |
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14091723 |
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61006457 |
Jan 15, 2008 |
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Current U.S.
Class: |
514/303 ;
514/394; 546/118; 548/305.4 |
Current CPC
Class: |
A61K 9/0019 20130101;
A61K 9/4866 20130101; C07D 471/04 20130101; A61K 9/0075 20130101;
A61P 31/12 20180101; A61K 9/2059 20130101; A61K 9/0014 20130101;
A61K 9/2054 20130101; A61K 9/02 20130101; A61K 31/4184 20130101;
A61K 9/0073 20130101; A61K 9/2027 20130101; C07D 235/20 20130101;
A61P 43/00 20180101; A61P 31/14 20180101 |
Class at
Publication: |
514/303 ;
548/305.4; 514/394; 546/118 |
International
Class: |
C07D 235/20 20060101
C07D235/20; C07D 471/04 20060101 C07D471/04 |
Goverment Interests
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
[0002] The research described herein was supported in part by funds
from the U.S. Government (Grant no. R43AI056525 and NIH SBIR grant
R44AI1056525) and the U.S. Government may therefore have certain
rights in the invention.
Claims
1. A pharmaceutical composition comprising a pharmaceutically
acceptable carrier and a compound having the following general
Formula I or a pharmaceutically acceptable salt thereof:
##STR00029## wherein B, D, E, G, B', D', E' and G' are
independently N; R is selected from the group consisting of
hydrogen, substituted or unsubstituted alkyl, alkenyl, alkynyl,
cycloalkyl, heterocycloalkyl, arylalkyl, aryl, heteroaryl, hydroxy,
alkyloxy, aryloxy, heteroaryloxy, acyloxy, arylacyloxy,
heteroarylacyloxy, alkylsulfonyloxy, arylsulfonyloxy, thio,
alkylthio, amino, alkylamino, dialkylamino, cycloalkylamino,
heterocycloalkylamino, arylamino, heteroarylamino, acylamino,
arylacylamino, heteroarylacylamino, alkylsulfonylamino,
arylsulfonylamino, acyl, arylacyl, heteroarylacyl, alkylsulfinyl,
arylsulfinyl, alkylsulfonyl, arylsulfonyl, aminosulfonyl,
substituted aminosulfonyl, carboxy, alkoxycarbonyl,
cycloalkyloxycarbonyl, aryloxycarbonyl, carbamoyl, substituted
carbamoyl, halogen, cyano, isocyano and nitro; and X and X' are
independently selected from the group consisting of O and S.
2. The composition of claim 1, wherein that at least one of B, D,
E, G, B', D', E' and G' is C--Y, wherein Y is halogen.
3. (canceled)
4. The composition of claim 1, wherein R is selected from the group
consisting of hydrogen, alkyl, amino and halogen.
5. The composition of claim 1, wherein, R is hydrogen or
methyl.
6. The composition of claim 1, wherein each of X and X' is
N--R'.
7. The composition of claim 6, wherein R' is hydrogen or alkyl.
8. The composition of claim 1, wherein the compound of Formula I is
selected from the group consisting of
1H,1'H-[2,2']Bibenzoimidazolyl;
5,6,5',6'-Tetramethyl-1H,1'H-[2,2']bibenzoimidazolyl;
1,1'-Dimethyl-1H,1'H-[2,2']bibenzoimidazolyl;
1,1'-Diethyl-1H,1'H-[2,2']bibenzoimidazolyl;
6,6'-Dinitro-1H,1'H-[2,2']bibenzoimidazolyl;
4,4'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dichloro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dibromo-1H,1'H-[2,2']bibenzoimidazolyl;
4,4'-Dimethyl-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dimethyl-1H,1'H-[2,2']bibenzoimidazolyl;
4,4'-Dimethoxy-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dimethoxy-1H,1'H-[2,2']bibenzoimidazolyl;
1H,1'H-[2,2']Bibenzoimidazolyl-4,4'-dicarboxylic acid dimethyl
ester; 1H,1'H-[2,2']Bibenzoimidazolyl-5,5'-dicarboxylic acid
dimethyl ester; 4,4'-Diphenyl-1H,1'H-[2,2']bibenzoimidazolyl;
4,5,4',5'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
4,6,4',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,6,5',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
1H,1'H-[2,2']Bi[imidazo[4,5-b]pyridinyl];
3H,3'H-[2,2']Bi[imidazo[4,5-c]pyridinyl];
4,7,4',7'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl; and
4,4'-Dichloro-1H,1'H-[2,2']bibenzoimidazolyl.
9. The composition of claim 1, wherein the compound of Formula I is
selected from the group consisting 1H,1'H-[2,2']Bibenzoimidazolyl;
4,4'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl; and
4,6,4',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl.
10. (canceled)
11. The compound of claim 10, wherein the compound of Formula I is
selected from the group consisting of
4,4'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dibromo-1H,1'H-[2,2']bibenzoimidazolyl;
4,5,4',5'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
4,6,4',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,6,5',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
4,7,4',7'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl; and
4,4'-Dichloro-1H,1'H-[2,2']bibenzoimidazolyl.
12. A pharmaceutical composition comprising a pharmaceutically
acceptable carrier and the compound of claim 1.
13. A compound or a pharmaceutically acceptable salt thereof
selected from the group consisting of
4,4'-Dimethoxy-1H,1'H-[2,2']bibenzoimidazolyl;
1H,1'H-[2,2']Bibenzoimidazolyl-4,4'-dicarboxylic acid dimethyl
ester; 1H,1'H-[2,2']Bibenzoimidazolyl-5,5'-dicarboxylic acid
dimethyl ester; and
4,4'-Diphenyl-1H,1'H-[2,2']bibenzoimidazolyl.
14. A pharmaceutical composition comprising a pharmaceutically
acceptable carrier and the compound of claim 13.
15.-36. (canceled)
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to and benefit of U.S.
Provisional Application No. 61/006,457, filed Jan. 15, 2008, the
contents of which are hereby incorporated by reference in their
entirety.
FIELD OF THE INVENTION
[0003] This invention relates to the use of 2,2'-bibenzimidazole,
2,2'-bibenzoxazole and 2,2'-bibenzothiazole derivatives and
analogs, as well as compositions containing the same, for the
treatment or prophylaxis of viral diseases associated with the
arenavirus family such as lymphocytic choriomeningitis virus
(LCMV).
BACKGROUND OF THE INVENTION
[0004] LCMV is the prototypic member of the Arenaviridae family, a
family of enveloped RNA viruses (2). Arenavirus infection in
rodents, the natural host animal, is usually chronic and
asymptomatic. Several arenaviruses can cause severe hemorrhagic
fever in humans, including Lassa, Machupo, Guanarito, and Junin
viruses. Transmission to humans can result from direct contact with
infected rodents or their habitat, through aerosolized rodent
secretions, or through contact with the body fluids of an infected
person. LCMV is found world-wide, reflecting the range of the Mus
musculus rodent host. The Arenaviridae family contains a single
genus (Arenavirus) that is divided into two major lineages based on
phylogenetic and serological examination. LCMV is a member of the
Old World arenaviruses, which includes the Lassa fever virus, a
Category A hemorrhagic fever virus.
[0005] In 1999, the Centers for Disease Control and Prevention
(CDC) identified and categorized potential biological terrorism
agents as part of a Congressional initiative to upgrade
bioterrorism response capabilities (12). Arenaviruses were
designated as Category A, defined as those pathogens with the
highest potential impact on public health and safety, potential for
large-scale dissemination, capability for civil disruption, and
greatest unmet need for public health preparedness. The National
Institute of Allergy and Infectious Diseases (NIAID) has continued
to update the Category A list (6), specifically listing LCMV as a
Category A virus (7).
[0006] Although LCMV is closely related to other arenaviruses known
to cause hemorrhagic fever, LCMV has been linked to only a very
limited number of cases in which patients exhibited hemorrhagic
fever-like symptoms (11). More often, exposure to the virus results
in a characteristic biphasic pattern of illness, beginning with
flu-like symptoms characterized by fever, headache, myalgia,
malaise, and fatigue after an incubation period of 1-3 weeks.
Following a few days of recovery, a second phase of disease can
occur in which patients exhibit symptoms of meningitis (fever,
headache, or stiff neck) or encephalitis (drowsiness, confusion,
sensory disturbances, and/or motor abnormalities). Studies
conducted between 1941 and 1958 at Walter Reed Medical Center show
that about 10% of febrile diseases with central nervous system
involvement were due to LCMV (1, 5). While meningitis and
encephalitis patients require hospitalization and supportive care
based on severity of symptoms, infected individuals usually recover
fully without sequelae, although nerve deafness and arthritis have
been reported. Mortality resulting from LCMV infection is rare,
occurring in less than 1% of cases (11).
[0007] LCMV can be transmitted from an infected pregnant woman to
the developing fetus resulting in severe teratogenic consequences
including hydrocephaly, microcephaly or macrocephaly, and
chorioretinitis (14). These conditions commonly lead to severe
developmental delays, mental retardation, blindness, and premature
death. The incidence of congenital LCMV infection among infants
born in the US has not been established (14).
[0008] Recently, new manifestations of LCMV-associated diseases
have been reported in the medical literature (3). In 2002 and again
in 2005, LCMV was transmitted to patients receiving
immunosuppressive medications after they received transplanted
organs from an infected donor. Out of eight organ recipients, seven
died from the infection. The donors exhibited no clinical signs of
LCMV infection and subsequent diagnostic investigations failed to
detect the virus. Nevertheless, the coincidence in timing and
phylogenetic matching of strains within each cluster provide
convincing evidence regarding the origins of the infecting agents
(3).
[0009] Prevention and treatment options for LCMV are limited. No
licensed vaccines or FDA-approved antiviral drugs are available.
Although ribavirin has shown limited antiviral activity against
LCMV in vitro, there exists no established data supporting its
routine use in humans. Thus there remains a need to develop safe
and effective products to protect against LCMV infections.
[0010] The arenavirus genome consists of two segments of
single-stranded RNA, each of which codes for two genes in opposite
orientations (referred to as ambisense). The larger of the two
segments, the L RNA (7.2 kb), encodes the L and Z proteins. The L
protein is the RNA-dependent RNA polymerase (RdRp), and the Z
protein is a small zinc-binding RING finger protein which is
involved in virus budding (10). The S RNA (3.4 kb) encodes the
nucleoprotein (NP) and the envelope glycoprotein precursor (GP-C).
The nucleocapsid consists of genomic RNA tightly encapsidated by
viral NP, the most abundant viral protein in virions and infected
cells.
[0011] The L protein is a circa 254 kDa multifunctional enzyme that
possesses characteristics shared with over 80 RdRps from
negative-strand viruses, including six conserved domains that
appear to correspond to essential functional features required to
synthesize RNA from RNA template (13). Viral RNA, NP, and L combine
to form a ribonucleoprotein complex, which transcribes and
replicates the viral genome (4). Because mammalian cells lack
homologs of the L and NP proteins, the potential to specifically
inhibit these enzymes without compromising essential host processes
make NP and L attractive targets for antiviral therapeutic
development efforts.
SUMMARY OF THE INVENTION
[0012] The present invention provides a pharmaceutical composition
comprising a pharmaceutically acceptable carrier and a compound
having the following general Formula I or a pharmaceutically
acceptable salt thereof:
##STR00001##
wherein B, D, E, G, B', D', E' and G' are independently N or
C--R;
[0013] R is selected from the group consisting of hydrogen,
substituted or unsubstituted alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, hydroxy, alkyloxy,
aryloxy, heteroaryloxy, acyloxy, arylacyloxy, heteroarylacyloxy,
alkylsulfonyloxy, arylsulfonyloxy, thio, alkylthio, amino,
alkylamino, dialkylamino, cycloalkylamino, heterocycloalkylamino,
arylamino, heteroarylamino, acylamino, arylacylamino,
heteroarylacylamino, alkylsulfonylamino, arylsulfonylamino, acyl,
arylacyl, heteroarylacyl, alkylsulfinyl, arylsulfinyl,
alkylsulfonyl, arylsulfonyl, aminosulfonyl, substituted
aminosulfonyl, carboxy, alkoxycarbonyl, cycloalkyloxycarbonyl,
aryloxycarbonyl, carbamoyl, substituted carbamoyl, halogen, cyano,
isocyano and nitro; and
[0014] X and X' are independently selected from the group
consisting of O, S or N--R', wherein R' is selected from the group
consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, acyl, arylacyl,
heteroarylacyl, sulfonyl, aminosulfonyl, substituted aminosulfonyl,
alkoxycarbonyl, cycloalkyloxycarbonyl, aryloxycarbonyl, carbamoyl
and substituted carbamoyl.
[0015] The present invention also provides a compound having the
following general Formula I or a pharmaceutically acceptable salt
thereof:
##STR00002##
wherein B, D, E, G, B', D', E' and G' are independently N or
C--R;
[0016] R is selected from the group consisting of hydrogen,
substituted or unsubstituted alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, hydroxy, alkyloxy,
aryloxy, heteroaryloxy, acyloxy, arylacyloxy, heteroarylacyloxy,
alkylsulfonyloxy, arylsulfonyloxy, thio, alkylthio, amino,
alkylamino, dialkylamino, cycloalkylamino, heterocycloalkylamino,
arylamino, heteroarylamino, acylamino, arylacylamino,
heteroarylacylamino, alkylsulfonylamino, arylsulfonylamino, acyl,
arylacyl, heteroarylacyl, alkylsulfinyl, arylsulfinyl,
alkylsulfonyl, arylsulfonyl, aminosulfonyl, substituted
aminosulfonyl, carboxy, alkoxycarbonyl, cycloalkyloxycarbonyl,
aryloxycarbonyl, carbamoyl, substituted carbamoyl, halogen, cyano,
isocyano and nitro; with a proviso that at least one of B, D, E, G,
B', D', B' and G' is C--Y, wherein Y is halogen, and [0017] (i) if
none of B, E, G, B', E' and G' is N, D and D' are not both C--F or
C--Cl; [0018] (ii) if none of D, E, G, D', E' and G' is N, B and B'
are not both C--Br; or [0019] (iii) if none of B, E, G, B', E' and
G' is N and when D is C--Br, D' is not C--Z, wherein Z is
4-methyl-1-piperazinyl; and
[0020] X and X' are independently selected from the group
consisting of O, S or N--R', wherein R' is selected from the group
consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, acyl, arylacyl,
heteroarylacyl, sulfonyl, aminosulfonyl, substituted aminosulfonyl,
alkoxycarbonyl, cycloalkyloxycarbonyl, aryloxycarbonyl, carbamoyl
and substituted carbamoyl.
[0021] The present invention further provides a compound or a
pharmaceutically acceptable salt thereof selected from the group
consisting of 4,4'-Dimethoxy-1H,1'H-[2,2']bibenzoimidazolyl;
1H,1'H-[2,2']Bibenzoimidazolyl-4,4'-dicarboxylic acid dimethyl
ester; 1H,1'H-[2,2']Bibenzoimidazolyl-5,5'-dicarboxylic acid
dimethyl ester; and
4,4'-Diphenyl-1H,1'H-[2,2']bibenzoimidazolyl.
[0022] The present invention also provides a method for the
treatment or prophylaxis of a viral infection or disease associated
therewith, comprising administering in a therapeutically effective
amount to a mammal in need thereof, a compound of Formula I below
or a pharmaceutically acceptable salt thereof:
##STR00003##
[0023] wherein B, D, E, G, B', D', E' and G' are independently N or
C--R;
[0024] R is selected from the group consisting of hydrogen,
substituted or unsubstituted alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, hydroxy, alkyloxy,
aryloxy, heteroaryloxy, acyloxy, arylacyloxy, heteroarylacyloxy,
alkylsulfonyloxy, arylsulfonyloxy, thio, alkylthio, amino,
alkylamino, dialkylamino, cycloalkylamino, heterocycloalkylamino,
arylamino, heteroarylamino, acylamino, arylacylamino,
heteroarylacylamino, alkylsulfonylamino, arylsulfonylamino, acyl,
arylacyl, heteroarylacyl, alkylsulfinyl, arylsulfinyl,
alkylsulfonyl, arylsulfonyl, aminosulfonyl, substituted
aminosulfonyl, carboxy, alkoxycarbonyl, cycloalkyloxycarbonyl,
aryloxycarbonyl, carbamoyl, substituted carbamoyl, halogen, cyano,
isocyano and nitro; and
[0025] X and X' are independently selected from the group
consisting of O, S or N--R', wherein R' is selected from the group
consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, acyl, arylacyl,
heteroarylacyl, sulfonyl, aminosulfonyl, substituted aminosulfonyl,
alkoxycarbonyl, cycloalkyloxycarbonyl, aryloxycarbonyl, carbamoyl
and substituted carbamoyl.
[0026] Other objects and advantages of the present invention will
become apparent from the following description and appended
claims.
DETAILED DESCRIPTION OF THE INVENTION
[0027] The compounds of the invention are of the following general
Formula I:
##STR00004##
wherein B, D, E, G, B', D', E' and G' are independently N or
C--R;
[0028] R is selected from the group consisting of hydrogen,
substituted or unsubstituted alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, hydroxy, alkyloxy,
aryloxy, heteroaryloxy, acyloxy, arylacyloxy, heteroarylacyloxy,
alkylsulfonyloxy, arylsulfonyloxy, thio, alkylthio, amino,
alkylamino, dialkylamino, cycloalkylamino, heterocycloalkylamino,
arylamino, heteroarylamino, acylamino, arylacylamino,
heteroarylacylamino, alkylsulfonylamino, arylsulfonylamino, acyl,
arylacyl, heteroarylacyl, alkylsulfinyl, arylsulfinyl,
alkylsulfonyl, arylsulfonyl, aminosulfonyl, substituted
aminosulfonyl, carboxy, alkoxycarbonyl, cycloalkyloxycarbonyl,
aryloxycarbonyl, carbamoyl, substituted carbamoyl, halogen, cyano,
isocyano and nitro; and
[0029] X and X' are independently selected from the group
consisting of O, S or N--R', wherein R' is selected from the group
consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl,
heterocycloalkyl, arylalkyl, aryl, heteroaryl, acyl, arylacyl,
heteroarylacyl, sulfonyl, aminosulfonyl, substituted aminosulfonyl,
alkoxycarbonyl, cycloalkyloxycarbonyl, aryloxycarbonyl, carbamoyl
and substituted carbamoyl.
[0030] Preferably each of B, D, E, G, B', D', E' and G' is C--R.
Also preferably, R is selected from the group consisting of
hydrogen, alkyl, amino and halogen. More preferably, R is hydrogen
or methyl.
[0031] Preferably, each of X and X' is N--R' wherein R' is hydrogen
or alkyl.
[0032] Preferably, the compound of the present invention is
selected from the group consisting of
1H,1'H-[2,2']Bibenzoimidazolyl;
5,6,5',6'-Tetramethyl-1H,1'H-[2,2']bibenzoimidazolyl;
1,1'-Dimethyl-1H,1'H-[2,2']bibenzoimidazolyl;
1,1'-Diethyl-1H,1'H-[2,2']bibenzoimidazolyl;
6,6'-Dinitro-1H,1'H-[2,2']bibenzoimidazolyl;
4,4'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dichloro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dibromo-1H,1'H-[2,2']bibenzoimidazolyl;
4,4'-Dimethyl-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dimethyl-1H,1'H-[2,2']bibenzoimidazolyl;
4,4'-Dimethoxy-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Dimethoxy-1H,1'H-[2,2']bibenzoimidazolyl;
1H,1'H-[2,2']Bibenzoimidazolyl-4,4'-dicarboxylic acid dimethyl
ester; 1H,1'H-[2,2']Bibenzoimidazolyl-5,5'-dicarboxylic acid
dimethyl ester; 4,4'-Diphenyl-1H,1'H-[2,2']bibenzoimidazolyl;
4,5,4',5'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
4,6,4',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,6,5',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl;
1H,1'H-[2,2']Bi[imidazo[4,5-b]pyridinyl];
3H,3'H-[2,2']Bi[imidazo[4,5-c]pyridinyl];
4,7,4',7'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl; and
4,4'-Dichloro-1H(1'H-[2,2']bibenzoimidazolyl.
[0033] More preferably, the compound of Formula I is
1H,1'H-[2,2']Bibenzoimidazolyl;
4,4'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl;
5,5'-Difluoro-1H,1'H-[2,2']bibenzoimidazolyl; or
4,6,4',6'-Tetrafluoro-1H,1'H-[2,2']bibenzoimidazolyl.
[0034] The method of the present invention is for the treatment or
prophylaxis of a viral infection or disease associated therewith,
comprising administering in a therapeutically effective amount to a
mammal in need thereof, a compound of Formula I described
above.
[0035] Preferably, the mammal is a human and the viral infection is
an arenavirus infection. More preferably, the arenavirus virus is
selected from the group consisting of Lassa, Junin, Machupo,
Guanarito, Sabia, Whitewater Arroyo, Chapare, LCMV, LCMV-like
viruses such as Dandenong, Tacaribe, and Pichinde.
[0036] Preferably, the viral infection is associated with a
condition selected from the group consisting of viral hemorrhagic
fever, meningitis, meningoencephalitis, encephalitis, or
arenavirus-mediated disease in organ transplant recipients.
Additionally, the patient could be a pregnant patient with a risk
of congenital or perinatal transmission of LCMV, in which the
desired outcome is the prevention or reduction of LCMV-associated
pathologies such as infant mortality, microcephaly, mental
retardation, chorioretinitis, and hydrocephalus.
[0037] The method of the present invention may also comprise
co-administration of: a) other antivirals such as ribavirin or
cidofovir; b) vaccines; and/or c) interferons or pegylated
interferons.
DEFINITIONS
[0038] In accordance with this detailed description, the following
abbreviations and definitions apply. It must be noted that as used
herein, the singular forms "a," "an," and "the" include plural
referents unless the context clearly dictates otherwise.
[0039] The publications discussed herein are provided solely for
their disclosure. Nothing herein is to be construed as an admission
regarding antedating the publications. Further, the dates of
publication provided may be different from the actual publication
dates, which may need to be independently confirmed.
[0040] Where a range of values is provided, it is understood that
each intervening value is encompassed. The upper and lower limits
of these smaller ranges may independently be included in the
smaller, subject to any specifically-excluded limit in the stated
range. Where the stated range includes one or both of the limits,
ranges excluding either both of those included limits are also
included in the invention. Also contemplated are any values that
fall within the cited ranges.
[0041] Unless defined otherwise, all technical and scientific terms
used herein have the same meaning as commonly understood by one of
ordinary skill in the art. Any methods and materials similar or
equivalent to those described herein can also be used in practice
or testing. All publications mentioned herein are incorporated
herein by reference to disclose and describe the methods and/or
materials in connection with which the publications are cited.
[0042] By "patient" or "subject" is meant to include any mammal. A
"mammal," for purposes of treatment, refers to any animal
classified as a mammal, including but not limited to, humans,
experimental animals including rats, mice, and guinea pigs,
domestic and farm animals, and zoo, sports, or pet animals, such as
dogs, horses, cats, cows, and the like.
[0043] The term "efficacy" as used herein refers to the
effectiveness of a particular treatment regime. Efficacy can be
measured based on change of the course of the disease in response
to an agent.
[0044] The term "success" as used herein in the context of a
chronic treatment regime refers to the effectiveness of a
particular treatment regime. This includes a balance of efficacy,
toxicity (e.g., side effects and patient tolerance of a formulation
or dosage unit), patient compliance, and the like. For a chronic
administration regime to be considered "successful" it must balance
different aspects of patient care and efficacy to produce a
favorable patient outcome.
[0045] The terms "treating," "treatment," and the like are used
herein to refer to obtaining a desired pharmacological and
physiological effect. The effect may be prophylactic in terms of
preventing or partially preventing a disease, symptom, or condition
thereof and/or may be therapeutic in terms of a partial or complete
cure of a disease, condition, symptom, or adverse effect attributed
to the disease. The term "treatment," as used herein, covers any
treatment of a disease in a mammal, such as a human, and includes:
(a) preventing the disease from occurring in a subject which may be
predisposed to the disease but has not yet been diagnosed as having
it, i.e., causing the clinical symptoms of the disease not to
develop in a subject that may be predisposed to the disease but
does not yet experience or display symptoms of the disease; (b)
inhibiting the disease, i.e., arresting or reducing the development
of the disease or its clinical symptoms; and (c) relieving the
disease, i.e., causing regression of the disease and/or its
symptoms or conditions. Treating a patient's suffering from disease
related to pathological inflammation is contemplated. Preventing,
inhibiting, or relieving adverse effects attributed to pathological
inflammation over long periods of time and/or are such caused by
the physiological responses to inappropriate inflammation present
in a biological system over long periods of time are also
contemplated.
[0046] As used herein, "acyl" refers to the groups H--C(O)--,
alkyl-C(O)--, substituted alkyl-C(O)--, alkenyl-C(O)--, substituted
alkenyl-C(O)--, alkynyl-C(O)--, substituted alkynyl-C(O)--,
cycloalkyl-C(O)--, substituted cycloalkyl-C(O)--, aryl-C(O)--,
substituted aryl-C(O)--, heteroaryl-C(O)--, substituted
heteroaryl-C(O)--, heterocyclic-C(O)--, and substituted
heterocyclic-C(O)-- wherein alkyl, substituted alkyl, alkenyl,
substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl,
substituted cycloalkyl, aryl, substituted aryl, heteroaryl,
substituted heteroaryl, heterocyclic and substituted heterocyclic
are as defined herein.
[0047] "Alkylamino" refers to the group --NRR where each R is
independently selected from the group consisting of hydrogen,
alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl,
substituted alkynyl, aryl, substituted aryl, cycloalkyl,
substituted cycloalkyl, heteroaryl, substituted heteroaryl,
heterocyclic, substituted heterocyclic and where each R is joined
to form together with the nitrogen atom a heterocyclic or
substituted heterocyclic ring wherein alkyl, substituted alkyl,
alkenyl, substituted alkenyl, alkynyl, substituted alkynyl,
cycloalkyl, substituted cycloalkyl, aryl, substituted aryl,
heteroaryl, substituted heteroaryl, heterocyclic and substituted
heterocyclic are as defined herein.
[0048] "Alkenyl" refers to alkenyl group preferably having from 2
to 10 carbon atoms and more preferably 2 to 6 carbon atoms and
having at least 1 and preferably from 1-2 sites of alkenyl
unsaturation.
[0049] "Alkoxy" refers to the group "alkyl-0-" which includes, by
way of example, methoxy, ethoxy, n-propoxy, iso-propoxy, n-butoxy,
tert-butoxy, sec-butoxy, n-pentoxy, n-hexoxy, 1,2-dimethylbutoxy,
and the like.
[0050] "Alkyl" refers to linear or branched alkyl groups having
from 1 to 10 carbon atoms, alternatively 1 to 6 carbon atoms. This
term is exemplified by groups such as methyl, t-butyl, n-heptyl,
octyl and the like.
[0051] "Amino" refers to the group --NH.sub.2.
[0052] "Aryl" or "Ar" refers to an unsaturated aromatic carbocyclic
group of from 6 to 14 carbon atoms having a single ring (e.g.,
phenyl) or multiple condensed rings (e.g., naphthyl or anthryl)
which condensed rings may or may not be aromatic (e.g.,
2-benzoxazolinone, 2H-1,4-benzoxazin-3(4H)-one, and the like)
provided that the point of attachment is through an aromatic ring
atom.
[0053] "Substituted aryl" refers to aryl groups which are
substituted with from 1 to 3 substituents selected from the group
consisting of hydroxy, acyl, acylamino, thiocarbonylamino, acyloxy,
alkyl, substituted alkyl, alkoxy, substituted alkoxy, alkenyl,
substituted alkenyl, alkynyl, substituted alkynyl, amidino,
alkylamidino, thioamidino, amino, aminoacyl, aminocarbonyloxy,
aminocarbonylamino, aminothiocarbonylamino, aryl, substituted aryl,
aryloxy, substituted aryloxy, cycloalkoxy, substituted cycloalkoxy,
heteroaryloxy, substituted heteroaryloxy, heterocyclyloxy,
substituted heterocyclyloxy, carboxyl, carboxylalkyl,
carboxyl-substituted alkyl, carboxyl-cycloalkyl,
carboxyl-substituted cycloalkyl, carboxylaryl, carboxyl-substituted
aryl, carboxylheteroaryl, carboxyl-substituted heteroaryl,
carboxylheterocyclic, carboxyl-substituted heterocyclic,
carboxylamido, cyano, thiol, thioalkyl, substituted thioalkyl,
thioaryl, substituted thioaryl, thioheteroaryl, substituted
thioheteroaryl, thiocycloalkyl, substituted thiocycloalkyl,
thioheterocyclic, substituted thioheterocyclic, cycloalkyl,
substituted cycloalkyl, guanidino, guanidinosulfone, halo, nitro,
heteroaryl, substituted heteroaryl, heterocyclic, substituted
heterocyclic, cycloalkoxy, substituted cycloalkoxy, heteroaryloxy,
substituted heteroaryloxy, heterocyclyloxy, substituted
heterocyclyloxy, oxycarbonylamino, oxythiocarbonylamino,
--S(O).sub.2-alkyl, --S(O).sub.2-substituted alkyl,
--S(O).sub.2-cycloalkyl, --S(O).sub.2-substituted cycloalkyl,
--S(O).sub.2-alkenyl, --S(O).sub.2-substituted alkenyl,
--S(O).sub.2-aryl, --S(O).sub.2-substituted aryl,
--S(O).sub.2-heteroaryl, --S(O).sub.2-substituted heteroaryl,
--S(O).sub.2-heterocyclic, --S(O).sub.2-substituted heterocyclic,
--OS(O).sub.2-alkyl, --OS(O).sub.2-substituted alkyl,
--OS(O).sub.2-aryl, --OS(O).sub.2-substituted aryl,
--OS(O).sub.2-heteroaryl, --OS(O).sub.2-substituted heteroaryl,
--OS(O).sub.2-heterocyclic, --OS(O).sub.2-substituted heterocyclic,
--OS(O).sub.2--NRR where R is hydrogen or alkyl,
--NRS(O).sub.2-alkyl, --NRS(O).sub.2-substituted alkyl,
--NRS(O).sub.2-aryl, --NRS(O).sub.2-substituted aryl,
--NRS(O).sub.2-heteroaryl, --NRS(O).sub.2-substituted heteroaryl,
--NRS(O).sub.2-heterocyclic, --NRS(O).sub.2-substituted
heterocyclic, --NRS(O).sub.2--NR-alkyl,
--NRS(O).sub.2--NR-substituted alkyl, --NRS(O).sub.2--NR-aryl,
--NRS(O).sub.2--NR-substituted aryl, --NRS(O).sub.2--NR-heteroaryl,
--NRS(O).sub.2--NR-substituted heteroaryl,
--NRS(O).sub.2--NR-heterocyclic, --NRS(O).sub.2--NR-substituted
heterocyclic where R is hydrogen or alkyl, mono- and di-alkylamino,
mono- and di-(substituted alkyl)amino, mono- and di-arylamino,
mono- and di-substituted arylamino, mono- and di-heteroarylamino,
mono- and di-substituted heteroarylamino, mono- and di-heterocyclic
amino, mono- and di-substituted heterocyclic amino, unsymmetric
di-substituted amines having different substituents independently
selected from the group consisting of alkyl, substituted alkyl,
aryl, substituted aryl, heteroaryl, substituted heteroaryl,
heterocyclic and substituted heterocyclic and amino groups on the
substituted aryl blocked by conventional blocking groups such as
Boc, Cbz, formyl, and the like or substituted with --SO.sub.2NRR
where R is hydrogen or alkyl.
[0054] "Cycloalkyl" refers to cyclic alkyl groups of from 3 to 8
carbon atoms having a single cyclic ring including, by way of
example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl,
cyclooctyl and the like. Excluded from this definition are
multi-ring alkyl groups such as adamantanyl, etc.
[0055] "Halo" or "halogen" refers to fluoro, chloro, bromo and
iodo.
[0056] "Heteroaryl" refers to an aromatic carbocyclic group of from
2 to 10 carbon atoms and 1 to 4 heteroatoms selected from the group
consisting of oxygen, nitrogen and sulfur within the ring or oxides
thereof. Such heteroaryl groups can have a single ring (e.g.,
pyridyl or furyl) or multiple condensed rings (e.g., indolizinyl or
benzothienyl) wherein one or more of the condensed rings may or may
not be aromatic provided that the point of attachment is through an
aromatic ring atom. Additionally, the heteroatoms of the heteroaryl
group may be oxidized, i.e., to form pyridine N-oxides or
1,1-dioxo-1,2,5-thiadiazoles and the like. Additionally, the carbon
atoms of the ring may be substituted with an oxo (.dbd.O). The term
"heteroaryl having two nitrogen atoms in the heteroaryl, ring"
refers to a heteroaryl group having two, and only two, nitrogen
atoms in the heteroaryl ring and optionally containing 1 or 2 other
heteroatoms in the heteroaryl ring, such as oxygen or sulfur.
[0057] "Substituted heteroaryl" refers to heteroaryl groups which
are substituted with from 1 to 3 substituents selected from the
group consisting of hydroxy, acyl, acylamino, thiocarbonylamino,
acyloxy, alkyl, substituted alkyl, alkoxy, substituted alkoxy,
alkenyl, substituted alkenyl, alkynyl, substituted alkynyl,
amidino, alkylamidino, thioamidino, amino, aminoacyl,
aminocarbonyloxy, aminocarbonylamino, aminothiocarbonylamino, aryl,
substituted aryl, aryloxy, substituted aryloxy, cycloalkoxy,
substituted cycloalkoxy, heteroaryloxy, substituted heteroaryloxy,
heterocyclyloxy, substituted heterocyclyloxy, carboxyl,
carboxylalkyl, carboxyl-substituted alkyl, carboxyl-cycloalkyl,
carboxyl-substituted cycloalkyl, carboxylaryl, carboxyl-substituted
aryl, carboxylheteroaryl, carboxyl-substituted heteroaryl,
carboxylheterocyclic, carboxyl-substituted heterocyclic,
carboxylamido, cyano, thiol, thioalkyl, substituted thioalkyl,
thioaryl, substituted thioaryl, thioheteroaryl, substituted
thioheteroaryl, thiocycloalkyl, substituted thiocycloalkyl,
thioheterocyclic, substituted thioheterocyclic, cycloalkyl,
substituted cycloalkyl, guanidino, guanidinosulfone, halo, nitro,
heteroaryl, substituted heteroaryl, heterocyclic, substituted
heterocyclic, cycloalkoxy, substituted cycloalkoxy, heteroaryloxy,
substituted heteroaryloxy, heterocyclyloxy, substituted
heterocyclyloxy, oxycarbonylamino, oxythiocarbonylamino,
--S(O).sub.2-alkyl, --S(O).sub.2-substituted alkyl,
--S(O).sub.2-cycloalkyl, --S(O).sub.2-substituted cycloalkyl,
--S(O).sub.2-alkenyl, --S(O).sub.2-substituted alkenyl,
--S(O).sub.2-aryl, --S(O).sub.2-substituted aryl,
--S(O).sub.2-heteroaryl, --S(O).sub.2-substituted heteroaryl,
--S(O).sub.2-heterocyclic, --S(O).sub.2-substituted heterocyclic,
--OS(O).sub.2-alkyl, --OS(O).sub.2-substituted alkyl,
--OS(O).sub.2-aryl, --OS(O).sub.2-substituted aryl,
--OS(O).sub.2-heteroaryl, --OS(O).sub.2-substituted heteroaryl,
--OS(O).sub.2-heterocyclic, --OS(O).sub.2-substituted heterocyclic,
--OSO.sub.2--NRR where R is hydrogen or alkyl,
--NRS(O).sub.2-alkyl, --NRS(O).sub.2-substituted alkyl,
--NRS(O).sub.2-aryl, --NRS(O).sub.2-substituted aryl,
--NRS(O).sub.2-heteroaryl, --NRS(O).sub.2-substituted heteroaryl,
--NRS(O).sub.2-heterocyclic, --NRS(O).sub.2-substituted
heterocyclic, --NRS(O).sub.2--NR-alkyl,
--NRS(O).sub.2--NR-substituted alkyl, --NRS(O).sub.2--NR-aryl,
--NRS(O).sub.2--NR-substituted aryl, --NRS(O).sub.2--NR-heteroaryl,
--NRS(O).sub.2--NR-substituted heteroaryl,
--NRS(O).sub.2--NR-heterocyclic, --NRS(O).sub.2--NR-substituted
heterocyclic where R is hydrogen or alkyl, mono- and di-alkylamino,
mono- and di-(substituted alkyl)amino, mono- and di-arylamino,
mono- and di-substituted arylamino, mono- and di-heteroarylamino,
mono- and di-substituted heteroarylamino, mono- and di-heterocyclic
amino, mono- and di-substituted heterocyclic amino, unsymmetric
di-substituted amines having different substituents independently
selected from the group consisting of alkyl, substituted alkyl,
aryl, substituted aryl, heteroaryl, substituted heteroaryl,
heterocyclic and substituted heterocyclic and amino groups on the
substituted aryl blocked by conventional blocking groups such as
Boc, Cbz, formyl, and the like or substituted with --SO.sub.2NRR
where R is hydrogen or alkyl.
[0058] "Sulfonyl" refers to the group --S(O).sub.2R where R is
selected from the group consisting of hydrogen, alkyl, substituted
alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl,
aryl, substituted aryl, cycloalkyl, substituted cycloalkyl,
heteroaryl, substituted heteroaryl, heterocyclic, substituted
heterocyclic wherein alkyl, substituted alkyl, alkenyl, substituted
alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted
cycloalkyl, aryl, substituted aryl, heteroaryl, substituted
heteroaryl, heterocyclic and substituted heterocyclic are as
defined herein.
[0059] "Optionally substituted" means that the recited group may be
unsubstituted or the recited group may be substituted.
[0060] "Pharmaceutically-acceptable carrier" means a carrier that
is useful in preparing a pharmaceutical composition or formulation
that is generally safe, non-toxic, and neither biologically nor
otherwise undesirable, and includes a carrier that is acceptable
for veterinary use as well as human pharmaceutical use.
[0061] "Pharmaceutically-acceptable cation" refers to the cation of
a pharmaceutically-acceptable salt.
[0062] "Pharmaceutically-acceptable salt" refers to salts which
retain the biological effectiveness and properties of compounds
which are not biologically or otherwise undesirable.
Pharmaceutically-acceptable salts refer to
pharmaceutically-acceptable salts of the compounds, which salts are
derived from a variety of organic and inorganic counter ions well
known in the art and include, by way of example only, sodium,
potassium, calcium, magnesium, ammonium, tetraalkylammonium, and
the like; and when the molecule contains a basic functionality,
salts of organic or inorganic acids, such as hydrochloride,
hydrobromide, tartrate, mesylate, acetate, maleate, oxalate and the
like.
[0063] Pharmaceutically-acceptable base addition salts can be
prepared from inorganic and organic bases. Salts derived from
inorganic bases include, by way of example only, sodium, potassium,
lithium, ammonium, calcium and magnesium salts. Salts derived from
organic bases include, but are not limited to, salts of primary,
secondary and tertiary amines, such as alkyl amines, dialkyl
amines, trialkyl amines, substituted alkyl amines, di(substituted
alkyl) amines, tri(substituted alkyl) amines, alkenyl amines,
dialkenyl amines, trialkenyl amines, substituted alkenyl amines,
di(substituted alkenyl) amines, tri(substituted alkenyl) amines,
cycloalkyl amines, di(cycloalkyl) amines, tri(cycloalkyl) amines,
substituted cycloalkyl amines, disubstituted cycloalkyl amine,
trisubstituted cycloalkyl amines, cycloalkenyl amines,
di(cycloalkenyl) amines, tri(cycloalkenyl) amines, substituted
cycloalkenyl amines, disubstituted cycloalkenyl amine,
trisubstituted cycloalkenyl amines, aryl amines, diaryl amines,
triaryl amines, heteroaryl amines, diheteroaryl amines,
triheteroaryl amines, heterocyclic amines, diheterocyclic amines,
triheterocyclic amines, mixed di- and tri-amines where at least two
of the substituents on the amine are different, and are selected
from the group consisting of alkyl, substituted alkyl, alkenyl,
substituted alkenyl, cycloalkyl, substituted cycloalkyl,
cycloalkenyl, substituted cycloalkenyl, aryl, heteroaryl,
heterocyclic, and the like. Also included are amines where the two
or three substituents, together with the amino nitrogen, form a
heterocyclic or heteroaryl group.
[0064] Examples of suitable amines include, by way of example only,
isopropylamine, trimethyl amine, diethyl amine, tri(iso-propyl)
amine, tri(n-propyl) amine, ethanolamine, 2-dimethylaminoethanol,
tromethamine, lysine, arginine, histidine, caffeine, procaine,
hydrabamine, choline, betaine, ethylenediamine, glucosamine,
N-alkylglucamines, theobromine, purines, piperazine, piperidine,
morpholine, N-ethylpiperidine, and the like. It should also be
understood that other carboxylic acid derivatives would be useful,
for example, carboxylic acid amides, including carboxamides, lower
alkyl carboxamides, dialkyl carboxamides, and the like.
[0065] Pharmaceutically-acceptable acid addition salts may be
prepared from inorganic and organic acids. Salts derived from
inorganic acids include hydrochloric acid, hydrobromic acid,
sulfuric acid, nitric acid, phosphoric acid, and the like. Salts
derived from organic acids include acetic acid, propionic acid,
glycolic acid, pyruvic acid, oxalic acid, malic acid, malonic acid,
succinic acid, maleic acid, fumaric acid, tartaric acid, citric
acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic
acid, ethanesulfonic acid, p-toluene-sulfonic acid, salicylic acid,
and the like.
[0066] A compound may act as a pro-drug. Pro-drug means any
compound which releases an active parent drug in vivo when such
pro-drug is administered to a mammalian subject. Pro-drugs are
prepared by modifying functional groups present in such a way that
the modifications may be cleaved in vivo to release the parent
compound. Pro-drugs include compounds wherein a hydroxy, amino, or
sulfhydryl group is bonded to any group that may be cleaved in vivo
to regenerate the free hydroxyl, amino, or sulfhydryl group,
respectively. Examples of pro-drugs include, but are not limited to
esters (e.g., acetate, formate, and benzoate derivatives),
carbamates (e.g., N,N-dimethylamino-carbonyl) of hydroxy functional
groups, and the like.
[0067] "Treating" or "treatment" of a disease includes:
(1) preventing the disease, i.e. causing the clinical symptoms of
the disease not to develop in a mammal that may be exposed to or
predisposed to the disease but does not yet experience or display
symptoms of the disease, (2) inhibiting the disease, i.e.,
arresting or reducing the development of the disease or its
clinical symptoms, or (3) relieving the disease, i.e., causing
regression of the disease or its clinical symptoms.
[0068] A "therapeutically-effective amount" means the amount of a
compound that, when administered to a mammal for treating a
disease, is sufficient to effect such treatment for the disease.
The "therapeutically-effective amount" will vary depending on the
compound, the disease, and its severity and the age, weight, etc.,
of the mammal to be treated.
Synthesis of Compounds
[0069] The compounds are readily prepared via several divergent
synthetic routes with the particular route selected relative to the
ease of compound preparation, the commercial availability of
starting materials, and the like.
[0070] The compounds can be prepared from readily-available
starting materials using the following general methods and
procedures. It will be appreciated that where process conditions
(i.e., reaction temperatures, times, mole ratios of reactants,
solvents, pressures, etc.) are given, other process conditions can
also be used unless otherwise stated. Optimum reaction conditions
may vary with the particular reactants or solvent used, but such
conditions can be determined by one skilled in the art by routine
optimization procedures.
[0071] Additionally, as will be apparent to those skilled in the
art, conventional protecting groups may be necessary to prevent
certain functional groups from undergoing undesired reactions.
Suitable protecting groups for various functional groups as well as
suitable conditions for protecting and deprotecting particular
functional groups are well known in the art. For example, numerous
protecting groups are described in T. W. Greene and G. M. Wuts,
Protecting Groups in Organic Synthesis, Second Edition, Wiley, New
York, 1991, and references cited therein.
[0072] Furthermore, the compounds may contain one or more chiral
centers. Accordingly, if desired, such compounds can be prepared or
isolated as pure stereoisomers, i.e., as individual enantiomers or
diastereomers, or as stereoisomer-enriched mixtures. All such
stereoisomers (and enriched mixtures) are included unless otherwise
indicated. Pure stereoisomers (or enriched mixtures) may be
prepared using, for example, optically-active starting materials or
stereoselective reagents well-known in the art. Alternatively,
racemic mixtures of such compounds can be separated using, for
example, chiral column chromatography, chiral resolving agents, and
the like.
[0073] Unless otherwise indicated, if the products contain chiral
centers, they are a mixture of R, S enantiomers. However, when a
chiral product is desired, the chiral product can be obtained via
purification techniques which separate enantiomers from a R, S
mixture to provide for one or the other stereoisomer. Such
techniques are known in the art.
[0074] The compounds can be provided as pro-drugs which convert
(e.g., hydrolyze, metabolize, etc.) in vivo to a compound
above.
Pharmaceutical Formulations of the Compounds
[0075] In general, compounds will be administered in a
therapeutically-effective amount by any of the accepted modes of
administration for these compounds. The compounds can be
administered by a variety of routes, including, but not limited to,
oral, parenteral (e.g., subcutaneous, subdural, intravenous,
intramuscular, intrathecal, intraperitoneal, intracerebral,
intraarterial, or intralesional routes of administration), topical,
intranasal, localized (e.g., surgical application or surgical
suppository), rectal, and pulmonary (e.g., aerosols, inhalation, or
powder). Accordingly, these compounds are effective as both
injectable and oral compositions. The compounds can be administered
continuously by infusion or by bolus injection.
[0076] The actual amount of the compound, i.e., the active
ingredient, will depend on a number of factors, such as the
severity of the disease, i.e., the condition or disease to be
treated, age, and relative health of the subject, the potency of
the compound used, the route and form of administration, and other
factors.
[0077] Toxicity and therapeutic efficacy of such compounds can be
determined by standard pharmaceutical procedures in cell cultures
or experimental animals, e.g., for determining the LD.sub.50 (the
dose lethal to 50% of the population) and the ED.sub.50 (the dose
therapeutically effective in 50% of the population). The dose ratio
between toxic and therapeutic effects is the therapeutic index and
it can be expressed as the ratio LD.sub.50/ED.sub.50.
[0078] The data obtained from the cell culture assays and animal
studies can be used in formulating a range of dosage for use in
humans. The dosage of such compounds lies within a range of
circulating concentrations that include the ED.sub.50 with little
or no toxicity. The dosage may vary within this range depending
upon the dosage form employed and the route of administration
utilized. For any compound used, the therapeutically-effective dose
can be estimated initially from cell culture assays. A dose may be
formulated in animal models to achieve a circulating plasma
concentration range which includes the IC.sub.50 (i.e., the
concentration of the test compound which achieves a half-maximal
inhibition of symptoms) as determined in cell culture. Such
information can be used to more accurately determine useful doses
in humans. Levels in plasma may be measured, for example, by high
performance liquid chromatography.
[0079] The amount of the pharmaceutical composition administered to
the patient will vary depending upon what is being administered,
the purpose of the administration, such as prophylaxis or therapy,
the state of the patient, the manner of administration, and the
like. In therapeutic applications, compositions are administered to
a patient already suffering from a disease in an amount sufficient
to cure or at least partially arrest the symptoms of the disease
and its complications. An amount adequate to accomplish this is
defined as "therapeutically-effective dose." Amounts effective for
this use will depend on the disease condition being treated as well
as by the judgment of the attending clinician depending upon
factors such as the severity of the inflammation, the age, weight,
and general condition of the patient, and the like.
[0080] The compositions administered to a patient are in the form
of 24 pharmaceutical compositions described supra. These
compositions may be sterilized by conventional sterilization
techniques, or may be sterile filtered. The resulting aqueous
solutions may be packaged for use as is, or lyophilized, the
lyophilized preparation being combined with a sterile aqueous
carrier prior to administration. It will be understood that use of
certain of the foregoing excipients, carriers, or stabilizers will
result in the formation of pharmaceutical salts.
[0081] The active compound is effective over a wide dosage range
and is generally administered in a pharmaceutically- or
therapeutically-effective amount. The therapeutic dosage of the
compounds will vary according to, for example, the particular use
for which the treatment is made, the manner of administration of
the compound, the health and condition of the patient, and the
judgment of the prescribing physician. For example, for intravenous
administration, the dose will typically be in the range of about
0.5 mg to about 100 mg per kilogram body weight. Effective doses
can be extrapolated from dose-response curves derived from in vitro
or animal model test systems. Typically, the clinician will
administer the compound until a dosage is reached that achieves the
desired effect.
[0082] When employed as pharmaceuticals, the compounds are usually
administered in the form of pharmaceutical compositions.
Pharmaceutical compositions contain as the active ingredient one or
more of the compounds above, associated with one or more
pharmaceutically-acceptable carriers or excipients. The excipient
employed is typically one suitable for administration to human
subjects or other mammals. In making the compositions, the active
ingredient is usually mixed with an excipient, diluted by an
excipient, or enclosed within a carrier which can be in the form of
a capsule, sachet, paper or other container. When the excipient
serves as a diluent, it can be a solid, semi-solid, or liquid
material, which acts as a vehicle, carrier, or medium for the
active ingredient. Thus, the compositions can be in the form of
tablets, pills, powders, lozenges, sachets, cachets, elixirs,
suspensions, emulsions, solutions, syrups, aerosols (as a solid or
in a liquid medium), ointments containing, for example, up to 10%
by weight of the active compound, soft and hard gelatin capsules,
suppositories, sterile injectable solutions, and sterile packaged
powders.
[0083] In preparing a formulation, it may be necessary to mill the
active compound to provide the appropriate Particle size prior to
combining with the other ingredients. If the active compound is
substantially insoluble, it ordinarily is milled to a particle size
of less than 200 mesh. If the active compound is substantially
water soluble, the particle size is normally adjusted by milling to
provide a substantially uniform distribution in the formulation,
e.g., about 40 mesh.
[0084] Some examples of suitable excipients include lactose,
dextrose, sucrose, sorbitol, mannitol, starches, gum acacia,
calcium phosphate, alginates, tragacanth, gelatin, calcium
silicate, microcrystalline cellulose, polyvinylpyrrolidone,
cellulose, sterile water, syrup, and methyl cellulose. The
formulations can additionally include: lubricating agents such as
talc, magnesium stearate, and mineral oil; wetting agents;
emulsifying and suspending agents; preserving agents such as
methyl- and propylhydroxy-benzoates; sweetening agents; and
flavoring agents. The compositions of the invention can be
formulated so as to provide quick, sustained, or delayed-release of
the active ingredient after administration to the patient by
employing procedures known in the art.
[0085] The quantity of active compound in the pharmaceutical
composition and unit dosage form thereof may be varied or adjusted
widely depending upon the particular application, the manner or
introduction, the potency of the particular compound, and the
desired concentration. The term "unit dosage forms" refers to
physically-discrete units suitable as unitary dosages for human
subjects and other mammals, each unit containing a predetermined
quantity of active material calculated to produce the desired
therapeutic effect, in association with a suitable pharmaceutical
excipient.
[0086] The compound can be formulated for parenteral administration
in a suitable inert carrier, such as a sterile physiological saline
solution. The dose administered will be determined by, route of
administration.
[0087] Administration of therapeutic agents by intravenous
formulation is well known in the pharmaceutical industry. An
intravenous formulation should possess certain qualities aside from
being just a composition in which the therapeutic agent is soluble.
For example, the formulation should promote the overall stability
of the active ingredient(s), also, the manufacture of the
formulation should be cost-effective. All of these factors
ultimately determine the overall success and usefulness of an
intravenous formulation.
[0088] Other accessory additives that may be included in
pharmaceutical formulations and compounds as follow: solvents:
ethanol, glycerol, propylene glycol; stabilizers: EDTA (ethylene
diamine tetraacetic acid), citric acid; antimicrobial
preservatives: benzyl alcohol, methyl paraben, propyl paraben;
buffering agents: citric acid/sodium citrate, potassium hydrogen
tartrate, sodium hydrogen tartrate, acetic acid/sodium acetate,
maleic acid/sodium maleate, sodium hydrogen phthalate, phosphoric
acid/potassium dihydrogen phosphate, phosphoric acid/disodium
hydrogen phosphate; and tonicity modifiers: sodium chloride,
mannitol, dextrose.
[0089] The presence of a buffer is necessary to maintain the
aqueous pH in the range of from about 4 to about 8. The buffer
system is generally a mixture of a weak acid and a soluble salt
thereof, e.g., sodium citrate/citric acid; or the monocation or
dication salt of a dibasic acid, e.g., potassium hydrogen tartrate;
sodium hydrogen tartrate, phosphoric acid/potassium dihydrogen
phosphate, and phosphoric acid/disodium hydrogen phosphate.
[0090] The amount of buffer system used is dependent on (1) the
desired pH; and (2) the amount of drug. Generally, the amount of
buffer used is able to maintain a formulation pH in the range of 4
to 8. Generally, a 1:1 to 10:1 mole ratio of buffer (where the
moles of buffer are taken as the combined moles of the buffer
ingredients, e.g., sodium citrate and citric acid) to drug is
used.
[0091] A useful buffer is sodium citrate/citric acid in the range
of 5 to 50 mg per ml. sodium citrate to 1 to 15 mg per ml. citric
acid, sufficient to maintain an aqueous pH of 4-6 of the
composition.
[0092] The buffer agent may also be present to prevent the
precipitation of the drug through soluble metal complex formation
with dissolved metal ions, e.g., Ca, Mg, Fe, Al, Ba, which may
leach out of glass containers or rubber stoppers or be present in
ordinary tap water. The agent may act as a competitive complexing
agent with the drug and produce a soluble metal complex leading to
the presence of undesirable particulates.
[0093] In addition, the presence of an agent, e.g., sodium chloride
in an amount of about of 1-8 mg/ml, to adjust the tonicity to the
same value of human blood may be required to avoid the swelling or
shrinkage of erythrocytes upon administration of the intravenous
formulation leading to undesirable side effects such as nausea or
diarrhea and possibly to associated blood disorders. In general,
the tonicity of the formulation matches that of human blood which
is in the range of 282 to 288 mOsm/kg, and in general is 285
mOsm/kg, which is equivalent to the osmotic pressure corresponding
to a 0.9% solution of sodium chloride.
[0094] An intravenous formulation can be administered by direct
intravenous injection, i.v. bolus, or can be administered by
infusion by addition to an appropriate infusion solution such as
0.9% sodium chloride injection or other compatible infusion
solution.
[0095] The compositions are preferably formulated in a unit dosage
form, each dosage containing from about 5 to about 100 mg, more
usually about 10 to about 30 mg, of the active ingredient. The term
"unit dosage forms" refers to physically discrete units suitable as
unitary dosages for human subjects and other mammals, each unit
containing a predetermined quantity of active material calculated
to produce the desired therapeutic effect, in association with a
suitable pharmaceutical excipient.
[0096] The active compound is effective over a wide dosage range
and is generally administered in a pharmaceutically effective
amount. It will be understood, however, that the amount of the
compound actually administered will be determined by a physician,
in the light of the relevant circumstances, including the condition
to be treated, the chosen route of administration, the actual
compound administered, the age, weight, and response of the
individual patient, the severity of the patient's symptoms, and the
like.
[0097] For preparing solid compositions such as tablets, the
principal active ingredient is mixed with a pharmaceutical
excipient to form a solid preformulation composition containing a
homogeneous mixture of a compound of the present invention. When
referring to these preformulation compositions as homogeneous, it
is meant that the active ingredient is dispersed evenly throughout
the composition so that the composition may be readily subdivided
into equally effective unit dosage forms such as tablets, pills and
capsules. This solid preformulation is then subdivided into unit
dosage forms of the type described above containing from, for
example, 0.1 to about 2000 mg of the active ingredient.
[0098] The tablets or pills may be coated or otherwise compounded
to provide a dosage form affording the advantage of prolonged
action. For example, the tablet or pill can comprise an inner
dosage and an outer dosage component, the latter being in the form
of an envelope over the former. The two components can be separated
by an enteric layer which serves to resist disintegration in the
stomach and permit the inner component to pass intact into the
duodenum or to be delayed in release. A variety of materials can be
used for such enteric layers or coatings, such materials including
a number of polymeric acids and mixtures of polymeric acids with
such materials as shellac, cetyl alcohol, and cellulose
acetate.
[0099] The liquid forms in which the novel compositions may be
incorporated for administration orally or by injection include
aqueous solutions, suitably flavored syrups, aqueous or oil
suspensions, and flavored emulsions with edible oils such as
cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as
elixirs and similar pharmaceutical vehicles.
[0100] Compositions for inhalation or insufflation include
solutions and suspensions in pharmaceutically-acceptable, aqueous
or organic solvents, or mixtures thereof, and powders. The liquid
or solid compositions may contain suitable
pharmaceutically-acceptable excipients as described supra.
Compositions in pharmaceutically-acceptable solvents may be
nebulized by use of inert gases. Nebulized solutions may be
breathed directly from the nebulizing device or the nebulizing
device may be attached to a face masks tent, or intermittent
positive pressure breathing machine. Solution, suspension, or
powder compositions may be administered from devices which deliver
the formulation in an appropriate manner.
[0101] The compounds can be administered in a sustained release
form. Suitable examples of sustained-release preparations include
semipermeable matrices of solid hydrophobic polymers containing the
compounds, which matrices are in the form of shaped articles, e.g.,
films, or microcapsules. Examples of sustained-release matrices
include polyesters, hydrogels (e.g.,
poly(2-hydroxyethyl-methacrylate) as described by Langer et al., J.
Biomed. Mater. Res. 15: 167-277 (1981) and Langer, Chem. Tech. 12:
98-105 (1982) or poly(vinyl alcohol)), polylactides (U.S. Pat. No.
3,773,919), copolymers of L-glutamic acid and gamma
ethyl-L-glutamate (Sidman et al., Biopolymers 22: 547-556, 1983),
non-degradable ethylene-vinyl acetate (Langer et al., supra),
degradable lactic acid-glycolic acid copolymers such as the LUPRON
DEPOT.TM. (i.e., injectable microspheres composed of lactic
acid-glycolic acid copolymer and leuprolide acetate), and
poly-D-(-)-3-hydroxybutyric acid (EP 133,988).
[0102] The compounds can be administered in a sustained-release
form, for example a depot injection, implant preparation, or
osmotic pump, which can be formulated in such a manner as to permit
a sustained-release of the active ingredient. Implants for
sustained-release formulations are well-known in the art. Implants
may be formulated as, including but not limited to, microspheres,
slabs, with biodegradable or non-biodegradable polymers. For
example, polymers of lactic acid and/or glycolic acid form an
erodible polymer that is well-tolerated by the host.
[0103] Transdermal delivery devices ("patches") may also be
employed. Such transdermal patches may be used to provide
continuous or discontinuous infusion of the compounds in controlled
amounts. The construction and use of transdermal patches for the
delivery of pharmaceutical agents is well known in the art. See,
e.g., U.S. Pat. No. 5,023,252, issued Jun. 11, 1991, herein
incorporated by reference. Such patches may be constructed for
continuous, pulsatile, or on-demand delivery of pharmaceutical
agents.
[0104] Direct or indirect placement techniques may be used when it
is desirable or necessary to introduce the pharmaceutical
composition to the brain. Direct techniques usually involve
placement of a drug delivery catheter into the host's ventricular
system to bypass the blood-brain barrier. One such implantable
delivery system used for the transport of biological factors to
specific anatomical regions of the body is described in U.S. Pat.
No. 5,011,472, which is herein incorporated by reference.
[0105] Indirect techniques usually involve formulating the
compositions to provide for drug latentiation by the conversion of
hydrophilic drugs into lipid-soluble drugs. Latentiation is
generally achieved through blocking of the hydroxy, carbonyl,
sulfate, and primary amine groups present on the drug to render the
drug more lipid-soluble and amenable to transportation across the
blood-brain barrier. Alternatively, the delivery of hydrophilic
drugs may be enhanced by intra-arterial infusion of hypertonic
solutions which can transiently open the blood-brain barrier.
[0106] In order to enhance serum half-life, the compounds may be
encapsulated, introduced into the lumen of liposomes, prepared as a
colloid, or other conventional techniques may be employed which
provide an extended serum half-life of the compounds. A variety of
methods are available for preparing liposomes, as described in,
e.g., Szoka et al., U.S. Pat. Nos. 4,235,871, 4,501,726 and
4,837,028 each of which is incorporated herein by reference.
[0107] Pharmaceutical compositions are suitable for use in a
variety of drug delivery systems. Suitable formulations for use in
the present invention are found in Remington's Pharmaceutical
Sciences, Mace Publishing Company, Philadelphia, Pa., 17th ed.
(1985).
[0108] The provided compounds and pharmaceutical compositions show
biological activity in treating and preventing viral infections and
associated diseases, and, accordingly, have utility in treating
viral infections and associated diseases, such as LCMV and
hemorrhagic fever viruses, in mammals including humans.
[0109] Hemorrhagic fever viruses (HFVs) are RNA viruses that cause
a variety of disease syndromes with similar clinical
characteristics. HFVs that are of concern as potential biological
weapons include but are not limited to: Arenaviridae (Junin,
Machupo, Guanarito, Sabia, and Lassa), Filoviridae (Ebola and
Marburg viruses), Flaviviridae (yellow fever, Omsk hemorrhagic
fever and Kyasanur Forest disease viruses), and Bunyaviridae (Rift
Valley fever and Crimean-Congo hemorrhagic fever). The
naturally-occurring arenaviruses and potential engineered
arenaviruses are included in the Category A Pathogen list according
to the Centers for Disease Control and Prevention as being among
those agents that have greatest potential for mass casualties.
[0110] Risk factors include: travel to Africa or Asia, handling of
animal carcasses, contact with infected animals or people, and/or
arthropod bites. Arenaviruses are highly infectious after direct
contact with infected blood and/or bodily secretions. Humans
usually become infected through contact with infected rodents, the
bite of an infected arthropod, direct contact with animal
carcasses, inhalation of infectious rodent excreta and/or injection
of food contaminated with rodent excreta. The Tacaribe virus has
been associated with bats. Airborne transmission of hemorrhagic
fever is another mode. Person-to-person contact may also occur in
some cases. LCMV is prevalent world-wide and associated with the
common house mouse Mus musculus, as well as certain other rodents
such as hamsters and guinea pigs. Risk factors include proximity to
mouse habitats or excreta, or exposure to pet rodents.
[0111] All of the hemorrhagic fevers exhibit similar clinical
symptoms. However, in general the clinical manifestations are
non-specific and variable. The incubation period is approximately
7-14 days. The onset is gradual with fever and malaise, tachypnea,
relative bradycardia, hypotension, circulatory shock, conjunctival
infection, pharyngitis, lymphadenopathy, encephalitis, myalgia,
back pain, headache and dizziness, as well as hyperesthesia of the
skin. Some infected patients may not develop hemorrhagic
manifestations.
[0112] Methods of diagnosis at specialized laboratories include
antigen detection by antigen-capture enzyme-linked immunosorbent
assay (ELISA), IgM antibody detection by antibody-capture
enzyme-linked immunosorbent assay, reverse transcriptase polymerase
chain reaction (RT-PCR), and viral isolation. Antigen detection (by
enzyme-linked immunosorbent assay) and reverse transcriptase
polymerase chain reaction are the most useful diagnostic techniques
in the acute clinical setting.
[0113] In the examples below, if an abbreviation is not defined
above, it has its generally accepted meaning. Further, all
temperatures are in degrees Celsius (unless otherwise indicated).
The following Methods were used to prepare the compounds set forth
below as indicated.
Example 1
General Synthetic Procedure
[0114] The bibenzimidazoles of this invention were obtained from
commercial sources or prepared following the procedures described
in J. Chem. Soc., Perkin Trans. I, 1967, 1, 20-25 and Inorg. Chem.
1978, 17, 2078-2083. The procedures are summarized in the following
synthetic scheme.
##STR00005##
[0115] Two equivalents of diamine reacting with one equivalent of
methyl 2,2,2-trichloroacetimidate or oximide yield the desired
product. The products can usually be isolated by precipitation into
water, followed by recrystallization or trituration with different
solvents. The products are then characterized with NMR, Mass
spectroscopy and/or melting point determination. The analytical
data are summarized in Table 1 below.
Example 2
Formulation 1
[0116] Hard gelatin capsules containing the following ingredients
are prepared:
TABLE-US-00001 Quantity Ingredient (mg/capsule) Active Ingredient
30.0 Starch 305.0 Magnesium stearate 5.0
[0117] The above ingredients are mixed and filled into hard gelatin
capsules in 340 mg quantities.
Example 3
Formulation 2
[0118] A tablet formula is prepared using the ingredients
below:
TABLE-US-00002 Quantity Ingredient (mg/capsule) Active ingredient
25.0 Cellulose, microcrystalline 200.0 Colloidal silicon dioxide
10.0 Stearic acid 5.0
[0119] The components are blended and compressed to form tablets,
each weighing 240 mg.
Example 4
Formulation 3
[0120] A dry powder inhaler formulation is prepared containing the
following components:
TABLE-US-00003 Ingredient Weight % Active Ingredient 5 Lactose
95
[0121] The active mixture is mixed with the lactose and the mixture
is added to a dry powder inhaling appliance.
Example 5
Formulation 4
[0122] Tablets, each containing 30 mg of active ingredient, are
prepared as follows:
TABLE-US-00004 Quantity Ingredient (mg/capsule) Active Ingredient
30.0 mg Starch 45.0 mg Microcrystalline cellulose 35.0 mg
Polyvinylpyrrolidone 4.0 mg (as 10% solution in water) Sodium
Carboxymethyl starch 4.5 mg Magnesium stearate 0.5 mg Talc 1.0 mg
Total 120 mg
[0123] The active ingredient, starch, and cellulose are passed
through a No. 20 mesh U.S. sieve and mixed thoroughly. The solution
of polyvinyl-pyrrolidone is mixed with the resultant powders, which
are then passed through a 16 mesh U.S. sieve. The granules so
produced are dried at 50.degree. to 60.degree. C. and passed
through a 16 mesh U.S. sieve. The sodium carboxymethyl starch,
magnesium stearate, and talc, previously passed through a No. 30
mesh U.S. sieve, are then added to the granules, which after
mixing, are compressed on a tablet machine to yield tablets each
weighing 150 mg.
Example 6
Formulation 5
[0124] Capsules, each containing 40 mg of medicament, are made as
follows:
TABLE-US-00005 Quantity Ingredient (mg/capsule) Active Ingredient
40.0 mg Starch 109.0 mg Magnesium stearate 1.0 mg Total 150.0
mg
[0125] The active ingredient, cellulose, starch, an magnesium
stearate are blended, passed through a No. 20 mesh U.S. sieve, and
filled into hard gelatin capsules in 150 mg quantities.
Example 7
Formulation 6
[0126] Suppositories, each containing 25 mg of active ingredient,
are made as follows:
TABLE-US-00006 Ingredient Amount Active Ingredient 25 mg Saturated
fatty acids glycerides to 2,000 mg
[0127] The active ingredient is passed through a No. 60 mesh U.S.
sieve and suspended in the saturated fatty acid glycerides
previously melted using the minimum heat necessary. The mixture is
then poured into a suppository mold of nominal 2.0 g capacity and
allowed to cool.
Example 8
Formulation 7
[0128] Suspensions, each containing 50 mg of medicament per 5.0 ml
dose, are made as follows:
TABLE-US-00007 Ingredient Amount Active Ingredient 50.0 mg Xanthan
gum 4.0 mg Sodium carboxymethyl cellose (11%) Microcrystalline
cellulose (89%) 500 mg Sucrose 1.75 g Sodium benzoate 10.0 mg
Flavor and color q.v. Purified water to 5.0 ml
[0129] The medicament, sucrose, and xanthan gum are blended, passed
through a NO. 10 mesh U.S. sieve, and then mixed with a previously
made solution of the microcrystalline cellulose and sodium
carboxymethyl cellulose in water. The sodium benzoate, flavor, and
color are diluted with some of the water and added with stirring.
Sufficient water is then added to produce the required volume.
Example 9
Formulation 8
[0130] Hard gelatin tablets, each containing 15 mg of active
ingredient, are made as follows:
TABLE-US-00008 Quantity Ingredient (mg/capsule) Active Ingredient
15.0 mg Starch 407.0 mg Magnesium stearate 3.0 mg Total 425.0
mg
[0131] The active ingredient, cellulose, starch, and magnesium
stearate are blended, passed through a No. 20 mesh U.S. sieve, and
filled into hard gelatin capsules in 560 mg quantities.
Example 10
Formulation 9
[0132] An intravenous formulation may be prepared as follows:
TABLE-US-00009 Ingredient mg/capsule) Active Ingredient 250.0 mg
Isotonic saline 1000 ml
[0133] Therapeutic compound compositions generally are placed into
a container having a sterile access port, for example, an
intravenous solution bag or vial having a stopper pierceable by a
hypodermic injection needle or similar sharp instrument.
Example 11
Formulation 10
[0134] A topical formulation may be prepared as follows:
TABLE-US-00010 Ingredient Quantity Active Ingredient 1-10 g
Emulsifying Wax 30 g Liquid Paraffin 20 g White Soft Paraffin to
100 g
[0135] The white soft paraffin is heated until molten. The liquid
paraffin and emulsifying wax are incorporated and stirred until
dissolved. The active ingredient is added and stirring is continued
until dispersed. The mixture is then cooled until solid.
Example 12
Formulation 11
[0136] An aerosol formulation may be prepared as follows: A
solution of the candidate compound in 0.5% sodium
bicarbonate/saline (w/v) at a concentration of 30.0 mg/mL is
prepared using the following procedure:
[0137] Preparation of 0.5% Sodium Bicarbonate/Saline Stock
Solution: 100.0 mL
TABLE-US-00011 Ingredient Gram/100.0 mL Final Concentration Sodium
Bicarbonate 0.5 g 0.5% Saline q.s. ad 100.0 mL q.s. ad 100%
[0138] Procedure:
1. Add 0.5 g sodium bicarbonate into a 100 mL volumetric flask. 2.
Add approximately 90.0 mL saline and sonicate until dissolved. 3.
Q.S. to 100.0 mL with saline and mix thoroughly.
[0139] Preparation of 30.0 mg/mL Candidate Compound: 10.0 mL
TABLE-US-00012 Ingredient Gram/100.0 mL Final Concentration
Candidate Compound 0.300 g 30.0 mg/mL .05% Sodium q.s. ad 10.0 mL
q.s. ad 100% Bicarbonte/Saline Stock Solution
[0140] Procedure:
1. Add 0.300 g of the candidate compound into a 10.0 mL volumetric
flask. 2. Add approximately 9.7 mL of 0.5% sodium
bicarbonate/saline stock solution. 3. Sonicate until the candidate
compound is completely dissolved. 4. Q.S. to 10.0 mL with 0.5%
sodium bicarbonate/saline stock solution and mix.
Example 13
Determining Antiviral Activity of Compounds of the Invention
[0141] Because LCMV forms noncytolytic infections (9), a cell-based
Enzyme-Linked ImmunoSorbent Assay (ELISA) was employed to screen a
small-molecule compound library for antiviral activity using the
Armstrong strain of LCMV (Arm53b). Briefly, Vero cell monolayers
were infected at a low multiplicity of infection in the presence of
test compounds at a concentration of 5 .mu.M. After a 5-day
incubation period, a monoclonal antibody was used to detect viral
NP within infected cells. The cell-based nature of this assay,
using authentic infectious LCMV, permitted detection of compounds
capable of inhibiting any process required for viral replication.
Compound 1 (see Table 1 below) was identified as a hit from this
screening and was further investigated for tractability, potency,
and selectivity.
[0142] A chemically tractable compound is defined as one that is
synthetically accessible using reasonable chemical methodology, and
which possesses chemically stable functionalities and potential
drug-like qualities. Compound 1 passed this medicinal chemistry
filter and was evaluated for potency. Compound potency was
determined by evaluating inhibitory activity across a broad range
of concentrations, again using the cell-based ELISA. Nonlinear
regression was used to generate best-fit inhibition curves and to
calculate a 50% effective concentration (EC.sub.50) equivalent to
80 nM. The selectivity or specificity of a given compound is
typically expressed as a ratio of its cytotoxicity to its
biological effect. A cell proliferation assay is used to calculate
a 50% cytotoxicity concentration (CC.sub.50); the ratio of this
value to the EC.sub.so is referred to as the therapeutic index
(T.I.=CC.sub.50/EC.sub.50). To determine cytotoxicity, a
fluorimetric assay that quantifies reduction of resazurin (Alamar
Blue) was employed (8). Compound 1 exhibited no quantifiable
cytotoxic effects at concentrations up to 25 .mu.M, making the T.I.
for this compound >310.
[0143] Broad evaluation of the mechanism of action for Compound 1
was performed. In LCMV-infected cells in the presence of 10 .mu.M
compound, synthesis of viral S RNA and NP mRNA were dramatically
suppressed relative to untreated controls for a period of at least
48 h post infection. Replication and transcription of viral RNA was
also evaluated by monitoring the activity of a reporter gene,
chloramphenicol acetyltransferase (CAT), encoded in a
negative-sense orientation on an RNA analog, the design of which is
based on the essential replicative elements of LCMV genomic RNA
(4). In this cell-based assay, replication and transcription of the
artificial RNA analog is facilitated by plasmid-encoded viral. NP
and L proteins. No CAT activity was observed in infected cells
treated with 20 .mu.M of Compound 1. Taken together, these results
suggest that Compound 1 inhibits the activity of either viral NP or
L protein, preventing efficient transcription and translation of
viral RNA.
[0144] Chemical analogs of this compound were obtained from
commercial vendors or were synthesized, and these analogs were
tested as described in order above to define the relationship
between chemical structure and biological activity. Several analogs
were tested for antiviral activities as shown in Table 1 below. Two
analogs, Compounds 6 and 7, displayed enhanced potency (EC.sub.50=6
nM and 63 nM, respectively) and selectivity relative to Compound
1.
TABLE-US-00013 TABLE 1 The anti-viral activity of compounds of the
present invention. Activity A: EC.sub.50 < 1 uM; B: 1 <
EC.sub.50 < 10 uM; C: 10 < EC.sub.50 < 25 uM; D: EC.sub.50
< 25 uM; Compound Chemical Structure Molecular Formula
Analytical Data Chemical Name n.d.: not determined 1 ##STR00006##
C14H10N4 .sup.1H NMR in DMSO-d6: .delta. 13.55 (s, 2H), 7.76 (d,
2H), 7.56 (d, 2H), 7.29 (td, 4H); .sup.13C NMR in DMSO-d6: .delta.
143.81, 143.49, 134.83, 123.63, 122.25, 119.18, 112.06; HRMS:
234.08976 M.sup.+ 1H,1'H- [2,2']Bibenzoimidazolyl A 2 ##STR00007##
C18H18N4 5,6,5',6'-Tetramethyl- 1H,1'H- [2,2']bibenzoimidazolyl B 3
##STR00008## C16H14N4 1,1'-Dimethyl-1H,1'H- [2,2']bibenzoimidazolyl
D 4 ##STR00009## C18H18N4 1,1'-Diethyl-1H,1'H-
[2,2']bibenzoimidazolyl D 5 ##STR00010## C14H8N6O4
6,6'-Dinitro-1H,1'H- [2,2']bibenzoimidazolyl D 6 ##STR00011##
C14H8F2N4 .sup.1H NMR in DMSO-d6: .delta. 14.04 (br s, 2H), 12.02
(br s, 1H), 7.26-7.45 (m, 4H), 7.07- 7.17 (m, 2H); Mass Spec: 269.3
(M - H).sup.- 4,4'-Difluoro-1H,1'H- [2,2']bibenzoimidazolyl A 7
##STR00012## C14H8F2N4 .sup.1H NMR in DMSO-d6: .delta. 7.76-7.82
(m, 1H), 7.54-7.68 (m, 2H), 7.12-7.34 (m, 3H); Mass Spec: 269.3 (M
- H).sup.- 5,5'-Difluoro-1H,1'H- [2,2']bibenzoimidazolyl A 8
##STR00013## C14H8Cl2N4 .sup.1H NMR in DMSO-d6: .delta. 7.71 (s,
2H), 7.67 (s, 2H), 7.35 (dd, 2H); Mass Spec: 301.2 (M - H).sup.-
5,5'-Dichloro-1H,1'H- [2,2']bibenzoimidazolyl B 9 ##STR00014##
C14H8Br2N4 .sup.1H NMR in DMSO-d6: .delta. 7.86 (s, 2H), 7.64 (d,
2H), 7.47 (dd, 2H); Mass Spec: 391.1 (M - H).sup.-
5,5'-Dibromo-1H,1'H- [2,2']bibenzoimidazolyl B 10 ##STR00015##
C16H14N4 .sup.1H NMR in DMSO-d6: .delta. 7.50 (d, 2H), 7.22 (t,
2H), 7.11 (d, 2H), 2.66 (s, 6H); Mass Spec: 363.2 (M + H).sup.+
4,4'-Dimethyl-1H,1'H- [2,2']bibenzoimidazolyl D 11 ##STR00016##
C16H14N4 .sup.1H NMR in DMSO-d6: .delta. 13.38 (br s, 1H), 12.00
(br s, 1H), 7.63 (d, 1H), 7.55 (s, 1H), 7.44 (d, 1H), 7.35 (s, 1H),
7.12 (t, 2H), 2.47 (s, 6H); Mass Spec: 363.3 (M + H).sup.+; 361.3
(M - H).sup.- 5,5'-Dimethyl-1H,1'H- [2,2']bibenzoimidazolyl D 12
##STR00017## C16H14N4O2 .sup.1H NMR in DMSO-d6: .delta. 7.21-7.36
(m, 4H), 6.89 (d, 2H), 4.01 (s, 6H); Mass Spec: 295.2 (M + H).sup.+
4,4'-Dimethoxy-1H,1'H- [2,2']bibenzoimidazolyl D 13 ##STR00018##
C16H14N4O2 .sup.1H NMR in DMSO-d6: .delta. 7.57 (d, 2H), 7.08 (s,
2H), 6.92 (dd, 2H), 3.84 (s, 6H); Mass Spec: 295.2 (M + H).sup.+
5,5'-Dimethoxy-1H,1'H- [2,2']bibenzoimidazolyl C 14 ##STR00019##
C18H14N4O4 .sup.1H NMR in DMSO-d6: .delta. 8.13 (d, 2H), 7.99 (d,
2H), 7.47 (t, 2H), 4.05 (s, 6H); Mass Spec: 351.0 (M + H).sup.+;
373.1 (M + Na).sup.+ 1H,1'H- [2,2']Bibenzoimidazolyl-
4,4'-dicarboxylic acid dimethyl ester D 15 ##STR00020## C18H14N4O4
.sup.1H NMR in DMSO-d6: .delta. 14.10 (br s, 1H), 11.99 (br s, 1H),
8.38 (s, 1H), 8.22 (s, 1H), 7.96 (m, 3H), 7.71 (d, 1H), 3.93 (s,
6H); Mass Spec: 349.3 (M - H).sup.- 1H,1'H-
[2,2']Bibenzoimidazolyl- 5,5'-dicarboxylic acid dimethyl ester D 16
##STR00021## C26H18N4 .sup.1H NMR in DMSO-d6: .delta. 8.04 (d, 4H),
7.38-7.64 (m, 12H); Melting point (uncorrected): 247-250.degree. C.
(decompose) 4,4'-Diphenyl-1H,1'H- [2,2']bibenzoimidazolyl D 17
##STR00022## C14H6F4N4 .sup.1H NMR in DMSO-d6: .delta. 7.37-7.44
(m, 4H) 4,5,4',5'-Tetrafluoro- 1H,1'H- [2,2']bibenzoimidazolyl A 18
##STR00023## C14H6F4N4 .sup.1H NMR in DMSO-d6: .delta. 14.17 (s,
1H), 12.00 (s, 1H), 7.13-7.26 (m, 4H) 4,6,4',6'-Tetrafluoro-
1H,1'H- [2,2']bibenzoimidazolyl A 19 ##STR00024## C14H6F4N4 .sup.1H
NMR in DMSO-d6: .delta. 7.48 (t, 4H); Mass Spec: 305.4 (M -
H).sup.- 5,6,5',6'-Tetrafluoro- 1H,1'H- [2,2']bibenzoimidazolyl A
20 ##STR00025## C12H8N6 1H,1'H-[2,2']Bi[imidazo[4,5- b]pyridinyl] A
21 ##STR00026## C12H8N6 Mass Spec: 237.3 (M + H).sup.+; Melting
point (uncorrected): >300.degree. C.
3H,3'H-[2,2']Bi[imidazo[4,5- b]pyridinyl] C 22 ##STR00027##
C14H6F4N4 4,7,4',7'-Tetrafluoro- 1H,1'H- [2,2']bibenzoimidazolyl
n.d. 23 ##STR00028## C14H8Cl2N4 4,4'-Dichloro-1H,1'H-
[2,2']bibenzoimidazolyl n.d.
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[0159] All references cited herein are herein incorporated by
reference in their entirety for all purposes.
[0160] The invention has been described in terms of preferred
embodiments thereof, but is more broadly applicable as will be
understood by those skilled in the art. The scope of the invention
is only limited by the following claims.
* * * * *
References