U.S. patent application number 14/105350 was filed with the patent office on 2014-04-10 for delivery of functional ingredients.
This patent application is currently assigned to NESTEC S.A .. The applicant listed for this patent is NESTEC S.A .. Invention is credited to Raymond Bertholet, Peter Bucheli, Pierre Ducret, Junkuan Wang, Herbert Johann Watzke.
Application Number | 20140099388 14/105350 |
Document ID | / |
Family ID | 34924518 |
Filed Date | 2014-04-10 |
United States Patent
Application |
20140099388 |
Kind Code |
A1 |
Wang; Junkuan ; et
al. |
April 10, 2014 |
DELIVERY OF FUNCTIONAL INGREDIENTS
Abstract
A primary composition comprising all essential nutrients of a
fruit or a plant material, which has an increased stability,
bio-availability and miscibility, and a process of forming same are
provided. An oral composition containing the primary composition in
a foodstuff, food supplement, cosmetic preparation or a
pharmaceutical preparation is also provided.
Inventors: |
Wang; Junkuan; (Lonay,
CH) ; Bertholet; Raymond; (Blonay, CH) ;
Watzke; Herbert Johann; (Lausanne, CH) ; Ducret;
Pierre; (St. Saphorin-sur-Morges, CH) ; Bucheli;
Peter; (Shanghai, CH) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
NESTEC S.A . |
Vevey |
|
CH |
|
|
Assignee: |
NESTEC S.A .
Vevey
CH
|
Family ID: |
34924518 |
Appl. No.: |
14/105350 |
Filed: |
December 13, 2013 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
10598909 |
Sep 14, 2006 |
|
|
|
PCT/EP05/02693 |
Mar 14, 2005 |
|
|
|
14105350 |
|
|
|
|
Current U.S.
Class: |
424/725 ;
426/565; 426/580; 426/583; 426/588; 426/589; 426/590; 426/594;
426/618; 426/648; 426/655; 426/656; 426/66 |
Current CPC
Class: |
A23V 2002/00 20130101;
A23L 2/39 20130101; A61Q 19/007 20130101; A23L 33/105 20160801;
A23L 33/19 20160801; A61K 8/11 20130101; A61K 8/345 20130101; A23K
20/20 20160501; A61K 8/9794 20170801; A61Q 19/005 20130101; A61K
8/9789 20170801; A61P 3/02 20180101; A61P 17/16 20180101; A61P
37/04 20180101; A23K 40/30 20160501; A61K 2800/92 20130101; A23K
20/174 20160501; A61K 8/986 20130101; A61Q 19/00 20130101; A61P
27/02 20180101; A23K 50/40 20160501; A61K 8/64 20130101; A23C
9/1307 20130101; A61P 9/00 20180101; A61P 27/12 20180101; A23K
20/10 20160501; A61P 3/10 20180101; A23J 1/20 20130101; A61P 17/00
20180101; A61P 3/00 20180101; A61Q 17/04 20130101; A23K 10/22
20160501; A23L 2/04 20130101; A61P 1/14 20180101; A61P 35/00
20180101; A23K 10/30 20160501; A61P 43/00 20180101; A61Q 19/08
20130101; A23V 2002/00 20130101; A23V 2250/21 20130101; A23V
2250/5424 20130101 |
Class at
Publication: |
424/725 ;
426/655; 426/648; 426/590; 426/580; 426/589; 426/66; 426/583;
426/588; 426/618; 426/565; 426/594; 426/656 |
International
Class: |
A23J 1/20 20060101
A23J001/20; A61K 8/64 20060101 A61K008/64; A23L 1/30 20060101
A23L001/30; A61K 8/98 20060101 A61K008/98 |
Foreign Application Data
Date |
Code |
Application Number |
Mar 19, 2004 |
EP |
04006639.1 |
Claims
1. A miscible primary composition comprising at least essential
lipophilic and hydrophilic bioactive components of a material
selected from the group consisting of whole fruit, vegetable
material, plant material, and combinations thereof, excluding
insoluble fibers, in a carrier selected from the group consisting
of milk, milk protein-containing carriers and combinations thereof,
wherein the essential lipophilic and hydrophilic bioactive
components are extracted from the material by milling the material
in the milk or milk protein-containing carrier and the insoluble
fibers are removed by centrifuging the carrier after milling and
wherein the miscible primary composition is stable, miscible and
dispersible in an aqueous system.
2. The miscible primary composition according to claim 1, wherein
the material is in a form selected from the group consisting of
vegetables, leaves, flowers, fruits, seeds and other parts of the
plant, and combinations thereof.
3. The miscible primary composition according to claim 1, wherein
the material is selected from the group consisting of a berry,
vegetables, seeds, flowers, citrus fruits, tomato, spinach, celery,
carrots, pea, kale, parsley, watercress, cabbage, broccoli,
lettuce, brussels sprouts, collard greens, turnip greens, fennel,
onions, tea, corn, cocoa, coffee, thyme, sweet red pepper, and
combinations thereof.
4. The miscible primary composition according to claim 1, wherein
the essential lipophilic and hydrophilic bioactive components are
selected from the group consisting of lipids, alkaloids, proteins,
carbohydrates, carotenoids, polyphenolic compounds, vitamins,
minerals, and combinations thereof.
5. The miscible primary composition according to claim 1, wherein
the essential lipophilic and hydrophilic bioactive components are
flavonoids selected from the group consisting of flavones,
flavonols, flavanones, catechins, anthocyanidins, isoflavones, and
combinations thereof.
6. The miscible primary composition according to claim 4, wherein
the carotenoids are selected from the group consisting of
carotenes, xanthophylls, and combinations thereof.
7. The miscible primary composition according to claim 1, wherein
the milk is from animal origin.
8. The miscible primary composition according to claim 1, which is
in a form selected from the group consisting of a powder, gel,
liquid, and combinations thereof.
9. The miscible primary composition according to claim 1, which
does not have acidic material.
10. An oral composition comprising a freeze-dried, miscible powder
comprising at least essential lipophilic and hydrophilic bioactive
components of a material selected from the group consisting of
whole fruit, vegetable material, plant material, and combinations
thereof, excluding insoluble fibers, in a milk or milk
protein-containing carrier, the oral composition in a form selected
from the group consisting of a foodstuff for oral administration, a
food supplement, a pet food product, a cosmetic preparation, a
pharmaceutical preparation, and combinations thereof, wherein the
essential lipophilic and hydrophilic bioactive components are
extracted from the material by milling the material in the milk or
milk protein-containing carrier and the insoluble fibers are
removed by centrifuging the carrier after milling and wherein the
freeze-dried, miscible powder is stable, miscible and dispersible
in an aqueous system.
11. The oral composition according to claim 10, which is a form
selected from the group consisting of a nutritional complete
formula, a dairy product, a chilled or shelf stable beverage, a
mineral or purified water, a liquid drink, a soup, a dietary
supplement, a meal replacement, a nutritional bar, a confectionery,
a milk or a fermented milk product, a yoghurt, a milk based powder,
an enteral nutrition product, an infant formula, an infant
nutritional product, a cereal product or a fermented cereal based
product, an ice-cream, a chocolate, coffee, a culinary product,
salad dressings, a pet food, and combinations thereof.
12. An oral, cosmetic or pharmaceutical composition comprising a
miscible primary composition comprising at least essential
lipophilic and hydrophilic bioactive components of a material
selected from the group consisting of whole fruit, vegetable, plant
material, and combinations thereof, excluding insoluble fibers, in
a milk or milk protein-containing carrier, the oral, cosmetic or
pharmaceutical composition being in a form selected from the group
consisting of a capsule, a pill, a solution, a suspension, a syrup,
a dried oral supplement, a wet oral supplement, and combinations
thereof, wherein the essential lipophilic and hydrophilic bioactive
components are extracted from the material by milling the material
in the milk or milk protein-containing carrier and the insoluble
fibers are removed by centrifuging the carrier after milling and
wherein the miscible primary composition is stable, miscible and
dispersible in an aqueous system.
13. The miscible primary composition according to claim 1, wherein
the material is selected from the group consisting of wolfberry,
blueberry, cranberry, white currant, red currant, blackcurrant,
mulberry, blackberry, gooseberry, raspberry, sea buckthorn,
strawberry, arbutus berry, grapes, flavonoid, polyphenol
carotenoid-rich fruit, vegetables, seeds, flowers, apples, melons,
kiwi, cherries, red date, prunes, peaches, persimmons, mandarin,
orange, tangerine, grapefruit, chamomile, chrysanthemum, bitter
orange, honeysuckle, jasmine and safflower, tomato, spinach,
celery, carrots, pea, kale, parsley, watercress, cabbage, broccoli,
lettuce, brussels sprouts, collard greens, turnip greens, fennel,
onions, tea, corn, cocoa, coffee, thyme, sweet red pepper, and
combinations thereof.
14. The miscible primary composition according to claim 1, wherein
the essential lipophilic and hydrophilic bioactive components are
selected from the group consisting of apigenin, luteolin,
diosmetin, quercetin, myricetin, kaempferol, naringenin,
hesperidin, epicatechin, gallocatechin, pelargonidin, malvidin,
cyaniding, genistein, daidzein, and combinations thereof.
15. A miscible primary composition comprising at least essential
lipophilic and hydrophilic bioactive components of a material
selected from the group consisting of whole fruit, vegetable
material, plant material, and combinations thereof, excluding
insoluble fibers, in a plant-based milk carrier, wherein the
essential lipophilic and hydrophilic bioactive components are
extracted from the material by milling the material in the
plant-based milk carrier and the insoluble fibers are removed by
centrifuging the carrier after milling and wherein the miscible
primary composition is stable, miscible and dispersible in an
aqueous system.
16. The miscible primary composition according to claim 15, wherein
the material is in a form selected from the group consisting of
vegetables, leaves, flowers, fruits, seeds and other parts of the
plant, and combinations thereof.
17. The miscible primary composition according to claim 15, wherein
the material is selected from the group consisting of a berry,
vegetables, seeds, flowers, citrus fruits, tomato, spinach, celery,
carrots, pea, kale, parsley, watercress, cabbage, broccoli,
lettuce, brussels sprouts, collard greens, turnip greens, fennel,
onions, tea, corn, cocoa, coffee, thyme, sweet red pepper, and
combinations thereof.
18. The miscible primary composition according to claim 15, wherein
the plant-based milk carrier is selected from the group consisting
of soymilk, coconut milk and combinations thereof.
19. The miscible primary composition according to claim 15, wherein
the essential lipophilic and hydrophilic bioactive components are
flavonoids selected from the group consisting of flavones,
flavonols, flavanones, catechins, anthocyanidins, isoflavones, and
combinations thereof.
20. The miscible primary composition according to claim 19, wherein
the carotenoids are selected from the group consisting of
carotenes, xanthophylls, and combinations thereof.
21. The miscible primary composition according to claim 15, which
is in a form selected from the group consisting of a powder, gel,
liquid, and combinations thereof.
22. The miscible primary composition according to claim 15, which
does not have acidic material.
Description
PRIORITY CLAIM
[0001] This application is a continuation application of U.S.
patent application Ser. No. 10/598,909, filed on Sep. 14, 2006,
which is a National Stage of International Application No.
PCT/EP2005/002693, filed Mar. 14, 2005, which claims priority to
European Application No. 04006639.1, filed Mar. 19, 2004, the
entire contents of which are being incorporated herein by
reference.
BACKGROUND
[0002] The present invention relates to a primary composition
comprising all essential nutrients of a fruit or a plant material,
which has an increased stability, bioavailability and miscibility;
and process of foaming the same. It also relates to an oral
composition that contains the primary composition in a foodstuff,
in a food supplement, in a cosmetic preparation or in a
pharmaceutical preparation.
[0003] Pigments and bioactive compounds extracted from fruits or
plant materials are widely used in the food industry as functional
ingredients. Among all of them, wolfberry (Lycium barbarum) is one
of the most valued functional ingredients in China, especially for
its benefits for eyesight, the immune system, and its anti-ageing
properties, associated with the multiple bioactive compounds
present in the fruit. It is traditionally consumed through hot
water extraction.
[0004] Many other ingredients are also well perceived by consumers
for their beneficial properties, but their applications in food
products are either difficult or give poor bioavailability. In
fact, fruits are usually rich in reducing sugars, making the drying
and handling of their powder very difficult.
[0005] A large numer of extraction techniques are already known.
For example, in WO03020053, a process for extracting carotenoids
from carotenoid-containing plant matter is described. It comprises
(i) Mixing the plant matter with water to achieve Brix not greater
than 10 DEG. (ii) Crushing the mixture from stage (i) and
separating the solids from the liquid to obtain two phases, pulp
and serum. (iii) Extracting the pulp to obtain
carotenoid-containing plant oleoresin. Such a water extraction
technique is natural, but is not as efficient as compared to the
use of a solvent.
[0006] For example, U.S. Pat. No. 6,648,564 describes a process for
forming, isolating and purifying xanthophyll crystals by
saponification of a xanthophyll diester-containing plant extract in
a composition of propylene glycol and aqueous alkali to form
xanthophyll crystals. The substantially pure xanthophyll crystals
so obtained are suitable for human consumption and can be used as a
nutritional supplement and as an additive in food. However, solvent
extraction techniques are more difficult to handle, and using
solvent can impair the natural image and/or nutritional functions
of the product.
[0007] Moreover, conventional extraction techniques usually extract
a few compounds of the plant or fruit material, leaving some other
bioactive compounds in the rest. For example, polysaccharides,
polyphenols and other non lipophilic compounds are not extracted
together with the lipophilic components such as carotenoids,
lipophilic vitamins and other lipids.
[0008] For example, U.S. Pat. No. 6,409,996 B1 describes a method
of obtaining a composition comprising one or more flavonoids by
treating a flavonoid containing raw material with an aqueous
extraction medium to obtain an extract and separating the
flavonoids from said extract by absorption and/or adsorption. Again
such extraction method only gives an extract mainly containing a
part of bioactive principles of the raw material.
[0009] It is thus an object of the present invention to address the
above problems by providing a formulation for extracting and
delivering the multi-nutrients from a fruit or a plant material
with improved stability, miscibility, dispersibility in aqueous
systems and enhanced bioavailability of the bioactive compounds. It
is also an object to provide a primary composition that can be used
directly or easily concentrated or dried into powder for
applications in food products, nutritional supplements, cosmetic or
pharmaceutical preparations.
SUMMARY
[0010] Accordingly, it is a first object of the invention to
provide a miscible primary composition comprising at least the
essential lipophilic and hydrophilic bioactive components of a
whole fruit, vegetable and/or plant material, excluding insoluble
fibers, in a milk or milk protein-containing carrier.
[0011] Advantageously, the primary composition has a close profile
of the essential active components of whole fruit, it has a good
stability, miscibility and dispersibility in aqueous systems.
Moreover, the primary composition has an enhanced nutritional
value, in the form of a better bioavailability and stability. It
has a pleasant taste and colour. It can be used directly or
concentrated or dried into powder for several applications into
daily-consumed food products or other nutritional uses.
[0012] Accordingly, in a further object the primary composition is
as an additive in a foodstuff for oral administration, such as in a
nutritional composition, a food supplement, a pet food product, a
cosmetic preparation or in a pharmaceutical preparation. The
primary composition is also an additive in a product for topical
application such as cosmetics or pharmaceutical products.
[0013] The invention also relates to methods of forming the primary
composition. The process for preparing the primary composition to
deliver the essential lipophilic and hydrophilic bioactive
components of a whole fruit, vegetable and/or plant material
comprises the steps of:
[0014] i) mixing and milling the fruit, vegetable and/or plant
material in milk or milk protein-containing liquid medium,
[0015] ii) separating insoluble fibers to obtain an aqueous
suspension
[0016] iii) optionally pasteurising the resulting suspension
[0017] iv) optionally add synthetic or natural bioactive components
(e.g. carotenoids) during the processing
[0018] v) and further optionally drying the suspension to obtain a
powder.
[0019] The process has the major advantage of being natural and
cost effective enabling improved delivery of multi-nutrients in the
form of a combination of stabilized water-and fat-soluble
compounds, free of organic solvent residues.
[0020] In a further aspect, the invention provides a method for
increasing miscibility or dispersibility in an aqueous system,
stability, and bioavailability of bioactive compounds of a fruit,
vegetable and/or a plant material using a process as described
above. In particular, by using milk or milk proteins, soymilk or
milk-like proteins from plants for extracting and delivering the
multi-nutrients of functional ingredients of a fruit, a vegetable
and/or a plant material.
[0021] It is another object of the invention to provide the use of
a primary composition as described above, for delivering the
multi-nutrients of functional ingredients of fruits, vegetables
and/or plant materials with improved bioavailability, miscibility
and stability.
[0022] It is a further object to provide the use of a primary
composition as described above for the preparation of an oral,
cosmetic or pharmaceutical composition intended for improving skin
health, in particular for photo protection of the skin or for
protecting skin tissue against ageing.
[0023] It is still a further object to provide the use of a primary
composition as described above for the preparation of an oral,
topical or pharmaceutical composition intended for eye health (e.g.
age-related macular degeneration).
[0024] The invention also provides the use of a primary composition
as described above for the preparation of a food or pharmaceutical
composition intended for stimulating the immune system.
[0025] Finally, the invention also provides the use of a primary
composition as described above for the preparation of a food or
pharmaceutical composition intended for treating diabetes (e.g.
having a hypoglycaemic effect).
[0026] The present invention now makes available to the consumer an
improved composition obtained from natural products. It provides a
primary composition retaining the important bioactive components of
a fruit or a plant material or mixture thereof. Its profile of the
bioactive components is close to that naturally occurred in the
fruit or the plant material. The primary composition provides
bioactive compounds of fruit or plant material, in a particularly
highly bioavailable, stable and miscible form.
[0027] The features of the present invention can be best understood
together with further objects and advantages by reference to the
following description.
DETAILED DESCRIPTION
[0028] Within the following description, the term bioactive
compound is understood to mean molecules or components showing
biological activity or health impact when orally ingested or
applied in cosmetics.
[0029] According to the first object, a miscible primary
composition comprising at least the essential lipophilic and
hydrophilic bioactive components of a whole fruit, vegetable and/or
plant material, excluding insoluble fibers, in a milk or milk
protein-containing carrier, is concerned.
[0030] In a preferred embodiment, the fruit, vegetable and/or plant
material may be in the form of vegetables, leaves, flowers, fruits,
seeds and other parts of the plant, or a mixture thereof.
[0031] In a preferred embodiment, berries or any other flavonoid,
polyphenol or carotenoid-rich fruit or vegetable or seeds are
selected. For example, berries such as wolfberry, blueberry,
cranberry, mulberry, blackberry, gooseberry, white currant,
blackcurrant, red currant, raspberry, sea buckthorn, strawberry,
arbutus berry or grapes and other fruits such as apples, melons,
kiwi, cherries, red date, prunes, peaches, persimmons, citrus
fruits such as mandarin, orange, tangerine, grapefruit, for
example, may be used. Flowers such as chamomile, chrysanthemum,
bitter orange, honeysuckle, jasmine and safflower may be used.
Vegetables such as tomato, spinach, celery, carrots, pea, kale,
parsley, watercress, cabbage, broccoli, lettuce, brussels sprouts,
collard greens, turnip greens, fennel, onions. Seeds such as corn,
black rice, cocoa, coffee and ingredients such as tea, thyme, sweet
red pepper, for example, may also be used.
[0032] Fruits, vegetables or plant materials may be used in the
form of fresh, concentrated or dried materials, for example, air or
freeze dried material.
[0033] The essential bioactive components of fruit, vegetable or
plant material may comprise lipids, alkaloids, proteins,
carbohydrates, carotenoids, polyphenolic compounds such as
flavonoids, and vitamins or minerals, for example. In particular,
the bioactive compounds may be flavonoids such as flavones (e.g.
apigenin, luteolin or diosmetin), flavonols (e.g. quercetin,
myricetin, kaempferol), flavanones (e.g. naringenin, hesperidin),
catechins (e.g. epicatechin, gallocatechin), anthocyanidins (e.g.
pelargonidin, malvidin, cyanidin) or isoflavones (e.g. genistein,
daidzein); carotenoids such as carotenes and xanthophylls (e.g.
lycopene, carotene, phytofluene, phytoene, canthaxanthin,
astaxanthin, beta-cryptoxanthin, capsanthin, lutein, zeaxanthin, or
those in the form of fatty acid esters; carbohydrates such as
arabinogalactan proteins (e.g. lycium barbarum polysaccharide);
vitamins (e.g. vitamin C, B, E . . . ); minerals (e.g. selenium,
calcium, magnesium, potassium).
[0034] The primary composition contains at least the essential
bioactive components of a whole fruit, vegetable and/or plant
material, excluding insoluble fibers, in a milk or milk
protein-containing carrier. The milk carrier may be in the form of
skimmed milk or whole milk from animal or plant origin (e.g.
soymilk, juice or coconut milk, etc.). In a more preferred
embodiment, cow's milk or soymilk are used, depending on the fruit
or primary composition which is desired. The milk-containing
carrier may be any edible liquid containing milk proteins such as
casein or whey proteins, for example. Vegetable oils may optionally
be added to the liquid medium.
[0035] The fruit, vegetable or plant material as described above,
is mixed and milled in said milk or milk protein-containing liquid
medium in a respective ratio of about 1:1 to 1:1000, preferably
from 1:5 to 1:50. The mixing and milling step may be carried out at
a temperature of from 1 to 95.degree. C., preferably from about 20
to 80.degree. C. and more preferably from 40 to 80.degree. C. Then,
insoluble fibers are removed to obtain an aqueous suspension. This
can be done by any conventional method. The resulting primary
composition may be further pasteurized and/or dried into a powder
by techniques known in the art. The primary composition obtained
may also be in liquid or gel form.
[0036] The present invention thus provides a primary composition
having a similar profile of the important nutrients like the whole
fruit, it has a good stability, miscibility and bioavailability.
These compositions may be highly dispersible in an aqueous system,
if the powder form is chosen. In this instance, the powder is
dispersible in cold or hot water.
[0037] The composition additionally comprises one or more of
emulsifiers, stabilizers, antioxidants and other additives. Use is
made of emulsifiers compatible in food, such as phospholipids, for
example lecithin, polyoxyethylene sorbitan mono- or tristearate,
monolaurate, monopahnitate, mono- or trioleate, a mono- or
diglyceride. Use may also be made of any type of stabilizer that is
known in food, in cosmetics or in pharmaceuticals. Use is made of
any type of antioxidants that is known in food, in cosmetics or in
pharmaceuticals. Use is made, as additives, of flavorings,
colorants and any other additive known in food, in cosmetics or in
pharmaceuticals. These emulsifiers, stabilizers, antioxidants and
additives are added according to the final use of the primary
composition.
[0038] The composition may also contain synthetic or natural
bioactive ingredients such as amino acids, fatty acids, vitamins,
minerals, carotenoids, polyphenols, etc. that can be added either
by dry or by wet mixing to said composition before pasteurization
and/or drying.
[0039] According to a further aspect, the present invention relates
to an oral composition comprising the primary composition described
above in a foodstuff, in a food supplement, in a pet food product,
in a cosmetic preparation or in a pharmaceutical preparation.
[0040] In a preferred embodiment, a food composition for human
consumption is supplemented by the above primary composition. This
composition may be a nutritional complete formula, a dairy product,
a chilled or shelf stable beverage, a mineral or purified water, a
liquid drink, a soup, a dietary supplement, a meal replacement, a
nutritional bar, a confectionery, a milk or a fermented milk
product, a yoghurt, a milk based powder, an enteral nutrition
product, an infant formula, an infant nutritional product, a cereal
product or a fermented cereal based product, an ice-cream, a
chocolate, coffee, a culinary product such as mayonnaise, tomato
puree or salad dressings or a pet food.
[0041] In this case, the primary composition, which is preferably
in the form of a powder, can be dispersed in the above-mentioned
foods or drinks so as to have a daily intake in bioactive nutrients
as described above, which depends mainly on the fruit, vegetable or
plant utilized, the desired effect and target tissue. The amount of
the primary composition or food composition to be consumed by the
individual to obtain a beneficial effect will also depend upon its
size, its type, and its age.
[0042] The nutritional supplement for oral administration may be in
capsules, gelatin capsules, soft capsules, tablets, sugar-coated
tablets, pills, pastes or pastilles, gums, or drinkable solutions
or emulsions, a syrup or a gel, with a dose of about 0.1 to 100% of
the primary composition, which can then be taken directly with
water or by any other known means. This supplement may also include
a sweetener, a stabilizer, an antioxidant, an additive, a flavoring
or a colorant. A supplement for cosmetic purpose can additionally
comprise a compound active with respect to the skin. Methods for
preparing them are common knowledge.
[0043] In another embodiment, a pharmaceutical composition can be
administered for prophylactic and/or therapeutic treatments. In
therapeutic applications, compositions are administered to a
patient already suffering from a disease, as described herein
under, in an amount sufficient to cure or at least partially arrest
the symptoms of the disease and its complications. An amount
adequate to accomplish this is defined as "a therapeutically
effective dose". Amounts effective for this will depend on the
severity of the disease and the weight and general state of the
patient. In prophylactic applications, compositions according to
the invention are administered to a patient susceptible to or
otherwise at risk of a particular disease. Such an amount is
defined to be "a prophylactic effective dose". In this use, the
precise amounts again depend on the patient's state of health and
weight.
[0044] The compounds of the invention are preferably administered
with a pharmaceutically acceptable carrier, the nature of the
carrier differing with the mode of administration, for example,
enteral, oral and topical (including ophthalmic) routes. The
desired formulation can be made using a variety of excipients
including, for example, pharmaceutical grades of mannitol, lactose,
starch, magnesium stearate, sodium saccharin, cellulose, magnesium
carbonate. This composition may be a tablet, a capsule, a pill, a
solution, a suspension, a syrup, a dried oral supplement, a wet
oral supplement.
[0045] It will be appreciated that the skilled person will, based
on his own knowledge select the appropriate components and galenic
form to target the active compound to the tissue of interest, e.g.
the skin, colon, stomach, eyes, kidney or liver, taking into
account the route of administration.
[0046] The invention also relates to a cosmetic composition
comprising the primary composition described above. It may be
formulated in lotions, shampoos, creams, sunscreens, after-sun
creams, anti-ageing creams and/or ointments, for example. This
composition which can be used topically additionally comprises a
fat or an oil which can be used in cosmetics, for example those
mentioned in the CTFA work, Cosmetic Ingredients Handbook,
Washington. It is also possible to add other cosmetically active
ingredients. The composition additionally comprises a structuring
agent and an emulsifier. Other excipients, colorants, fragrances or
opacifiers can also be added to the composition. It will be
appreciated that the present cosmetic products will contain a
mixture of different ingredients known to the skilled person,
ensuring a fast penetration of the said substance into the skin and
preventing degradation thereof during storage.
[0047] It will be understood that the concept of the present
invention may likewise be applied as an adjuvant therapy assisting
in presently used medications. Since the compounds of the present
invention may easily be administered together with food material
special clinical food may be applied containing a high amount of
the said substances. It will be clear that on reading the present
specification together with the appending claims the skilled person
will envisage a variety of different alternatives to the specific
embodiments mentioned herein.
[0048] Administering to a pet or human, a food, nutritional
supplement, a cosmetic or pharmaceutical composition as described
above, results in an improved skin health, in particular for photo
protection of the skin or for protecting skin tissue against
ageing, e.g. for inhibiting damage to the skin and/or mucous
membranes by inhibiting collagenases and enhancing the synthesis of
collagen. In fact, the use of the primary composition as described
above makes it possible to enhance the bioavailability of the said
bioactive compounds in the body and to slow down the ageing of the
skin, for example. It may also be useful in the prevention or
treatment of sensible, dry or reactive skins, or for improving skin
density or firmness.
[0049] The primary composition as described above may also be used
for the preparation of an oral, topical or pharmaceutical
composition for eyesight, in particular for reducing risk of
cataract and age-related macular degeneration. It can be used also
for preventing or treating cardiovascular diseases or disorders or
cancers and stimulating the immune system, and reducing blood
glucose, for example.
[0050] The following examples illustrate the invention in more
detail without restricting the same thereto. All percentages are
given by weight otherwise indicated.
EXAMPLES
Example 1
Preparation of the Primary Composition from Wolfberry
[0051] Dried wolfberry fruits (40 g) and whole milk (300 g) were
introduced in a 1-liter container. The mixture was kept to stand
for 10 minutes and treated with Polytron (Dispersing and Mixing
Technology by KINEMATICA, PT3000) at 26000 rpm for 15 minutes under
a nitrogen atmosphere. During the Polytron-treatment the
temperature of the mixture was maintained at 80-85.degree. C. by
means of water bath and cooled to room temperature afterwards. The
resulting mixture was then centrifuged at 2000 G for 10 minutes.
The solid residue is discarded. The liquid phase (306 g of
orange-yellow milk) was freeze dried. The dried product is finally
grinded to give 54 g of orange-yellow powder, which had shown very
god water-dispersible property and improved stability of zeaxanthin
compared to the wolfberry fruit powder.
Example 2
Preparation of the Primary Composition from Tomato
[0052] Fresh tomato pulp (93 g), skimmed milk powder (10 g) and tap
water (30 g) were introduced in a 250-ml container. The mixture was
homogenized (Polytron) at 26000 rpm for 10 minutes under a nitrogen
atmosphere. The temperature of the mixture was maintained below
30.degree. C. by cooling with an ice bath. The mixture was then
centrifuged at 2000 G for 10 minutes. The solid residue was
discarded and the liquid phase (96 g of a red-pink milk) was freeze
dried. The dried product was finally grinded and a red pink powder
(17.5g) was obtained.
Example 3
Dairy Product Containing Primary Composition According to the
Invention
[0053] The primary composition as prepared in example 1, is used
for the manufacture of fermented yogurt-like milk products. To do
this, IL of a milk product containing 2.8% of fats and supplemented
with 2% of skimmed milk powder and 6% of sucrose was prepared,
pasteurized and its temperature then lowered to 42.degree. C.
Precultures of a non-thickening strain of Streptococcus
thermophilus and of a non-viscous strain of Lactobacillus
bulgaricus were reactivated in a sterile MSK culture medium
containing 10% of reconstituted milk powder and 0.1% of commercial
yeast extract. The pasteurized milk product is then inoculated with
1% of each of these reactivated precultures and this milk product
was then allowed to ferment at 32.degree. C. until the pH reached a
value of 4.5. To the fermented milk, yogurt-like product, the
primary composition as in example 1 (1%) was added and stored at
4.degree. C.
Example 4
Pet Food Product
[0054] A feed mixture was made up of corn, corn gluten, chicken and
fish, salts, vitamins and minerals. The moistened feed leaving the
pre-conditioner was then fed into an extruder-cooker and
gelatinised. The gelatinised matrix leaving the extruder was forced
through a die and extruded. The extrudate leaving the die head was
cut into pieces suitable for feeding to dogs, dried at about
110.degree. C. for about 20 minutes, and cooled to form pellets.
The resulting water activity of the pellets was about 0.6.
[0055] The pellets were coated by spraying a coating substrate
comprising tallow fat and the primary composition as prepared in
example 1.
Example 5
Cosmetic for Oral Administration
[0056] A composition in the form of a hard capsule has the
following formulation:
TABLE-US-00001 TABLE 1 Compound mg per capsule Primary composition
of example 500 Excipient for the core Microcrystalline cellulose 70
Encompress TM 60 Magnesium stearate 3 Anhydrous colloidal silica 1
Coating agent Gum-lac 5 Talc 61 Sucrose 250 Polyvidone 6 Titanium
dioxide 0.3 Coloring agent 5
[0057] The composition can administered to the individual in an
amount of 2 to 3 capsules daily.
Example 6
The bioavailability of Zeaxanthin from Wolfberry Preparation
[0058] Two different wolfberry preparations were prepared as the
following:
[0059] Treatment A
[0060] 2 kg of dried wolfberry fruit was rinsed with tap water and
added to 12 L of milk (reconstituted with 1 kg of skimmed milk
powder) at 30.degree. C. under mixing with Polytron (Dispersing and
Mixing Technology by KINEMATICA, PT120/4M). The temperature of the
mixture was raised to 80.degree. C. and kept for 15 minutes under a
nitrogen atmosphere. During the Polytron-treatment the temperature
of the mixture was maintained at 80-85.degree. C. by means of water
bath and cooled to room temperature afterwards. The resulting
mixture was then centrifuged at 2000 G for 10 minutes. The solid
residue was discarded. After the addition of 1.3 kg of maltodextrin
(IT06), the liquid phase was freeze-dried. The dried product was
finally grinded to give 3 kg of orange-yellow powder.
[0061] Treatment B
[0062] 0.5 kg of dried wolfberry was grinded into powder together
with 0.5 kg of dry ice using Kenwood and Buhler grinder. The powder
mixture was kept for 1 hour in an oven at ambient temperature under
vacuum. The resulting powder was introduced into 0.8 L of water
(83.degree. C.) in a doubled jacket reactor equipped with an
agitator. After the temperature was raised back to 80.degree. C.,
the resulting suspension was subjected to a thermal treatment for
about 7 minutes under nitrogen. Then the suspension was
freeze-dried to give a pasteurized wolfberry powder.
[0063] Treatment C
[0064] Optisharp 5% CWS/S-TG is a reddish, fine granular powder,
which contains synthetic zeaxanthin finely dispersed in a
cornstarch-coated matrix of modified food starch, from DSM.
[0065] A clinical study was conducted with these three preparations
in which 12 persons consumed each preparation (both adjusted to 15
mg of endogenous zeaxanthin) in a three-week interval, together
with sufficient amounts of oil and carbohydrates in a meal.
[0066] It was observed that the absorption of zeaxanthin from the
wolfberry obtained with treatment A was significantly better than
that found for treatment B (Table 2), as measured in the
chylomicron fraction of blood samples taken over a time of 10 hours
post-ingestion. A similar absorption of zeaxanthin was also
observed with the treatment C.
TABLE-US-00002 TABLE 2 The bioavailability of zeaxanthin from
wolfberry preparations and formulated synthetic zeaxanthin. Cmax
nmol/l AUC amol/l*h Treatment (unadjusted for baseline (0 time)
Baseline adjusted A 1.72 (0.46) 9.73 (2.45) B 0.69 (0.21) 3.14
(1.09) C 1.86 (0.39) 8.34 (1.69)
Example 7
Preparation of the Primary Composition from Wolfberry
[0067] 3 kg of dried wolfberry fruit was rinsed with tap water and
added to 18 L of milk (reconstituted with 0.85 kg of skimmed milk
powder) at 30.degree. C. under mixing with Polytron (Dispersing and
Mixing Technology by KINEMATICA, PT120/4M). The temperature of the
mixture was maintained at 30.degree. C. and the treatment with the
Polytron continued for 30 minutes under a nitrogen atmosphere. The
resulting mixture was then centrifuged at 2000G for 10 minutes. The
solid residue is discarded. After the addition of 2 kg of
maltodextrin (IT06), the liquid phase was pasteurized and then
freeze-dried. The dried product was finally grinded to give 4.4 kg
of orange-red powder, which had very good water-dispersible
property and improved stability of zeaxanthin compared to the
wolfberry fruit powder.
* * * * *