U.S. patent application number 13/993235 was filed with the patent office on 2014-03-27 for novel 7beta-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid. This patent application is currently assigned to Pharmazell GMBH. The applicant listed for this patent is Arno Aigner, Daniel Bakonyi, Michael Braun, Sven Bresch, Werner Hummel, Christina Kantzow, Boqiao Sun, Dirk Weuster-Botz. Invention is credited to Arno Aigner, Daniel Bakonyi, Michael Braun, Sven Bresch, Werner Hummel, Christina Kantzow, Boqiao Sun, Dirk Weuster-Botz.
Application Number | 20140087421 13/993235 |
Document ID | / |
Family ID | 44063960 |
Filed Date | 2014-03-27 |
United States Patent Application | 20140087421 |
Kind Code | A1 |
Weuster-Botz; Dirk ; et al. | March 27, 2014 |
The invention relates to novel 7.beta.-hydroxysteroid dehydrogenase mutants, to the sequences which encode these enzyme mutants, to processes for the preparation of the enzyme mutants and to their use in enzymatic reactions of cholic acid compounds, in particular in the preparation of ursodeoxycholic acid (UDCS). The invention also relates to novel processes for the synthesis of UDCS using the enzyme mutants; and to the preparation of UDCS using recombinant, multiply-modified microorganisms.
Inventors: | Weuster-Botz; Dirk; (Munich, DE) ; Braun; Michael; (Bad Mergentheim-Edelfingen, DE) ; Aigner; Arno; (Tuntenhausen, DE) ; Sun; Boqiao; (Munich, DE) ; Kantzow; Christina; (Munich, DE) ; Bresch; Sven; (Munich, DE) ; Bakonyi; Daniel; (Koln, DE) ; Hummel; Werner; (Titz, DE) | ||||||||||
Applicant: |
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Assignee: | Pharmazell GMBH Raubling DE |
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Family ID: | 44063960 | ||||||||||
Appl. No.: | 13/993235 | ||||||||||
Filed: | December 16, 2011 | ||||||||||
PCT Filed: | December 16, 2011 | ||||||||||
PCT NO: | PCT/EP11/73141 | ||||||||||
371 Date: | November 19, 2013 |
Current U.S. Class: | 435/61 ; 435/190; 435/252.2; 435/252.3; 435/252.33; 435/252.34; 435/252.35; 536/23.2 |
Current CPC Class: | C12P 33/00 20130101; C12N 9/0006 20130101; C12N 9/0008 20130101; C12P 33/02 20130101; C12Y 101/01201 20130101 |
Class at Publication: | 435/61 ; 435/190; 536/23.2; 435/252.33; 435/252.34; 435/252.35; 435/252.2; 435/252.3 |
International Class: | C12N 9/04 20060101 C12N009/04; C12P 33/02 20060101 C12P033/02 |
Date | Code | Application Number |
---|---|---|
Dec 16, 2010 | EP | 10015726.2 |
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 69 <210> SEQ ID NO 1 <211> LENGTH: 792 <212>
TYPE: DNA <213> ORGANISM: Collinsella aerofaciens <220>
FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(792)
<400> SEQUENCE: 1 atg aac ctg agg gag aag tac ggt gag tgg ggc
ctg atc ctg ggc gcg 48 Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly
Leu Ile Leu Gly Ala 1 5 10 15 acc gag ggc gtc ggc aag gcg ttc tgc
gag aag atc gcc gcc ggc ggc 96 Thr Glu Gly Val Gly Lys Ala Phe Cys
Glu Lys Ile Ala Ala Gly Gly 20 25 30 atg aac gtc gtc atg gtc ggc
cgt cgc gag gag aag ctg aac gtg ctc 144 Met Asn Val Val Met Val Gly
Arg Arg Glu Glu Lys Leu Asn Val Leu 35 40 45 gca ggc gag atc cgc
gag acc tac ggc gtg gag acc aag gtc gtg cgc 192 Ala Gly Glu Ile Arg
Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60 gcc gac ttt
agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc 240 Ala Asp Phe
Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65 70 75 80 gag
ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc 288 Glu
Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 85 90
95 ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc
336 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile
100 105 110 aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac
atg cgg 384 Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr
Met Arg 115 120 125 atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac
gtc tcg tcg atg 432 Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn
Val Ser Ser Met 130 135 140 acc ggc atc agc tcc agc ccc tgg aac ggc
cag tac ggc gcg ggc aag 480 Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly
Gln Tyr Gly Ala Gly Lys 145 150 155 160 gcc ttc atc ctc aag atg acc
gag gcc gtg gcc tgc gag tgc gag ggc 528 Ala Phe Ile Leu Lys Met Thr
Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175 acc ggc gtc gac gtc
gag gtc atc acc ctc ggc acc acc cta acc ccc 576 Thr Gly Val Asp Val
Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185 190 agc ctg ctg
tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg 624 Ser Leu Leu
Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 195 200 205 aag
atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag 672 Lys
Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210 215
220 ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc
720 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser
225 230 235 240 gtc cac gac tgg aag gca aac cac acc gag gac gag tac
atc cgc tac 768 Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr
Ile Arg Tyr 245 250 255 atg ggg tcg ttc tac cgc gac tag 792 Met Gly
Ser Phe Tyr Arg Asp 260 <210> SEQ ID NO 2 <211> LENGTH:
263 <212> TYPE: PRT <213> ORGANISM: Collinsella
aerofaciens <400> SEQUENCE: 2 Met Asn Leu Arg Glu Lys Tyr Gly
Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu Gly Val Gly Lys
Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30 Met Asn Val Val
Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu 35 40 45 Ala Gly
Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65
70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu
His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His
Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val Thr Phe Leu Lys Cys
Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln Asp Arg Gly
Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr Gly Ile Ser Ser Ser
Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160 Ala Phe Ile
Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175 Thr
Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185
190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met
195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe
Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg
Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp Lys Ala Asn His Thr
Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe Tyr Arg Asp
260 <210> SEQ ID NO 3 <211> LENGTH: 31 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 3
gggaattcca tatgaacctg agggagaagt a 31 <210> SEQ ID NO 4
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 4 cccaagcttc tagtcgcggt agaacga 27
<210> SEQ ID NO 5 <211> LENGTH: 774 <212> TYPE:
DNA <213> ORGANISM: Comamonas testosteroni <220>
FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(774)
<400> SEQUENCE: 5 atg tcc atc atc gtg ata agc ggc tgc gcc acc
ggc att ggt gcc gct 48 Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr
Gly Ile Gly Ala Ala 1 5 10 15 acg cgc aag gtc ctg gag gcg gcc ggt
cac cag atc gta ggc atc gat 96 Thr Arg Lys Val Leu Glu Ala Ala Gly
His Gln Ile Val Gly Ile Asp 20 25 30 ata cgc gat gcg gaa gtg att
gcc gat ctc tcg acg gcc gaa ggt cga 144 Ile Arg Asp Ala Glu Val Ile
Ala Asp Leu Ser Thr Ala Glu Gly Arg 35 40 45 aag cag gcg att gcc
gat gta ctg gcg aag tgc agc aag ggc atg gac 192 Lys Gln Ala Ile Ala
Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp 50 55 60 ggc ctg gtg
ctg tgc gcc ggc ctg gga ccg cag acc aag gtg ctt ggc 240 Gly Leu Val
Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly 65 70 75 80 aat
gtg gtt tcg gtc aat tat ttt ggc gcg acc gag ctg atg gat gcc 288 Asn
Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala 85 90
95 ttt ttg cca gcg ctg aaa aaa ggc cat cag ccc gca gcc gtc gtc atc
336 Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile
100 105 110 tcg tcc gtg gct tcc gcg cat ctg gct ttt gac aag aac cca
ctg gcg 384 Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro
Leu Ala 115 120 125 ctg gca ctg gaa gcc ggc gag gaa gcc aag gcc cgc
gcc att gtc gaa 432 Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg
Ala Ile Val Glu 130 135 140 cat gcg gga gag cag ggc gga aat ctg gcc
tat gcg ggc agc aag aat 480 His Ala Gly Glu Gln Gly Gly Asn Leu Ala
Tyr Ala Gly Ser Lys Asn 145 150 155 160 gct ttg acg gtg gct gtg cgc
aaa cgc gcc gcc gcc tgg ggc gag gct 528 Ala Leu Thr Val Ala Val Arg
Lys Arg Ala Ala Ala Trp Gly Glu Ala 165 170 175 ggc gtg cgc ctg aac
acc atc gcc ccc ggt gca acc gag act ccc ttg 576 Gly Val Arg Leu Asn
Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu 180 185 190 ctg cag gcg
ggc ctg cag gac ccg cgc tat ggc gaa tcc att gcc aag 624 Leu Gln Ala
Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys 195 200 205 ttc
gtt cct ccc atg ggc cgc cgt gcc gag ccg tcc gag atg gcg tcg 672 Phe
Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser 210 215
220 gtc atc gcc ttt ttg atg agc ccg gcc gca agc tat gtg cat ggc gcg
720 Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala
225 230 235 240 cag atc gtc att gat ggc ggc att gat gcg gtg atg cgc
ccg aca cag 768 Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg
Pro Thr Gln 245 250 255 ttc tga 774 Phe <210> SEQ ID NO 6
<211> LENGTH: 257 <212> TYPE: PRT <213> ORGANISM:
Comamonas testosteroni <400> SEQUENCE: 6 Met Ser Ile Ile Val
Ile Ser Gly Cys Ala Thr Gly Ile Gly Ala Ala 1 5 10 15 Thr Arg Lys
Val Leu Glu Ala Ala Gly His Gln Ile Val Gly Ile Asp 20 25 30 Ile
Arg Asp Ala Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg 35 40
45 Lys Gln Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp
50 55 60 Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val
Leu Gly 65 70 75 80 Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu
Leu Met Asp Ala 85 90 95 Phe Leu Pro Ala Leu Lys Lys Gly His Gln
Pro Ala Ala Val Val Ile 100 105 110 Ser Ser Val Ala Ser Ala His Leu
Ala Phe Asp Lys Asn Pro Leu Ala 115 120 125 Leu Ala Leu Glu Ala Gly
Glu Glu Ala Lys Ala Arg Ala Ile Val Glu 130 135 140 His Ala Gly Glu
Gln Gly Gly Asn Leu Ala Tyr Ala Gly Ser Lys Asn 145 150 155 160 Ala
Leu Thr Val Ala Val Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala 165 170
175 Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu
180 185 190 Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile
Ala Lys 195 200 205 Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser
Glu Met Ala Ser 210 215 220 Val Ile Ala Phe Leu Met Ser Pro Ala Ala
Ser Tyr Val His Gly Ala 225 230 235 240 Gln Ile Val Ile Asp Gly Gly
Ile Asp Ala Val Met Arg Pro Thr Gln 245 250 255 Phe <210> SEQ
ID NO 7 <211> LENGTH: 969 <212> TYPE: DNA <213>
ORGANISM: Rattus norvegicus <220> FEATURE: <221>
NAME/KEY: CDS <222> LOCATION: (1)..(969) <400>
SEQUENCE: 7 atg gat tcc ata tct ctg cgt gta gca cta aat gat ggt aac
ttc att 48 Met Asp Ser Ile Ser Leu Arg Val Ala Leu Asn Asp Gly Asn
Phe Ile 1 5 10 15 cct gta ctg ggg ttt gga acc act gtg cct gag aag
gtt gct aag gat 96 Pro Val Leu Gly Phe Gly Thr Thr Val Pro Glu Lys
Val Ala Lys Asp 20 25 30 gaa gtt atc aag gct act aaa ata gct ata
gat aat gga ttc cgc cat 144 Glu Val Ile Lys Ala Thr Lys Ile Ala Ile
Asp Asn Gly Phe Arg His 35 40 45 ttt gac tct gct tat ttg tac gaa
gta gaa gag gaa gtg ggc caa gcc 192 Phe Asp Ser Ala Tyr Leu Tyr Glu
Val Glu Glu Glu Val Gly Gln Ala 50 55 60 att aga agc aag att gaa
gac ggc act gtg aag aga gaa gat ata ttc 240 Ile Arg Ser Lys Ile Glu
Asp Gly Thr Val Lys Arg Glu Asp Ile Phe 65 70 75 80 tat act tca aag
ctt tgg agc act ttc cat aga cca gag ctg gtc cga 288 Tyr Thr Ser Lys
Leu Trp Ser Thr Phe His Arg Pro Glu Leu Val Arg 85 90 95 act tgc
ttg gaa aag aca ctg aaa agc act caa ctg gac tat gtg gat 336 Thr Cys
Leu Glu Lys Thr Leu Lys Ser Thr Gln Leu Asp Tyr Val Asp 100 105 110
ctt tat att att cat ttc cca atg gct ttg cag cct gga gat ata ttt 384
Leu Tyr Ile Ile His Phe Pro Met Ala Leu Gln Pro Gly Asp Ile Phe 115
120 125 ttc cca cga gat gag cat gga aaa cta ttg ttt gaa aca gtg gat
atc 432 Phe Pro Arg Asp Glu His Gly Lys Leu Leu Phe Glu Thr Val Asp
Ile 130 135 140 tgt gac aca tgg gag gcc atg gaa aag tgt aag gat gca
gga ttg gcc 480 Cys Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp Ala
Gly Leu Ala 145 150 155 160 aag tct att ggg gtg tcc aac ttt aac tgc
agg cag ctg gag agg att 528 Lys Ser Ile Gly Val Ser Asn Phe Asn Cys
Arg Gln Leu Glu Arg Ile 165 170 175 ctg aat aag cca ggg ctc aaa tac
aag cct gtg tgc aac cag gtg gaa 576 Leu Asn Lys Pro Gly Leu Lys Tyr
Lys Pro Val Cys Asn Gln Val Glu 180 185 190 tgt cac ctt tat ctc aac
cag agc aaa atg ctg gac tat tgt aag tca 624 Cys His Leu Tyr Leu Asn
Gln Ser Lys Met Leu Asp Tyr Cys Lys Ser 195 200 205 aaa gac atc att
ctg gtt tcc tac tgc acg ctg gga agt tca cga gac 672 Lys Asp Ile Ile
Leu Val Ser Tyr Cys Thr Leu Gly Ser Ser Arg Asp 210 215 220 aaa aca
tgg gtg gat cag aaa agt cca gtt ctc cta gat gat cca gtt 720 Lys Thr
Trp Val Asp Gln Lys Ser Pro Val Leu Leu Asp Asp Pro Val 225 230 235
240 ctt tgt gcc ata gca aag aag tac aag caa acc cca gcc cta gtt gcc
768 Leu Cys Ala Ile Ala Lys Lys Tyr Lys Gln Thr Pro Ala Leu Val Ala
245 250 255 ctt cgc tac cag ctg cag cgt ggg gtt gtg ccc ctg atc agg
agt ttc 816 Leu Arg Tyr Gln Leu Gln Arg Gly Val Val Pro Leu Ile Arg
Ser Phe 260 265 270 aac gcg aag cgg atc aaa gag cta aca cag gtt ttt
gaa ttc cag ttg 864 Asn Ala Lys Arg Ile Lys Glu Leu Thr Gln Val Phe
Glu Phe Gln Leu 275 280 285 gct tca gag gac atg aaa gcc ctg gat ggc
ttg aac aga aat ttc aga 912 Ala Ser Glu Asp Met Lys Ala Leu Asp Gly
Leu Asn Arg Asn Phe Arg 290 295 300 tac aac aat gca aaa tat ttt gat
gac cat ccc aat cat cca ttt act 960 Tyr Asn Asn Ala Lys Tyr Phe Asp
Asp His Pro Asn His Pro Phe Thr 305 310 315 320 gat gaa tag 969 Asp
Glu <210> SEQ ID NO 8 <211> LENGTH: 322 <212>
TYPE: PRT <213> ORGANISM: Rattus norvegicus <400>
SEQUENCE: 8 Met Asp Ser Ile Ser Leu Arg Val Ala Leu Asn Asp Gly Asn
Phe Ile 1 5 10 15 Pro Val Leu Gly Phe Gly Thr Thr Val Pro Glu Lys
Val Ala Lys Asp 20 25 30 Glu Val Ile Lys Ala Thr Lys Ile Ala Ile
Asp Asn Gly Phe Arg His 35 40 45 Phe Asp Ser Ala Tyr Leu Tyr Glu
Val Glu Glu Glu Val Gly Gln Ala 50 55 60 Ile Arg Ser Lys Ile Glu
Asp Gly Thr Val Lys Arg Glu Asp Ile Phe 65 70 75 80 Tyr Thr Ser Lys
Leu Trp Ser Thr Phe His Arg Pro Glu Leu Val Arg 85 90 95 Thr Cys
Leu Glu Lys Thr Leu Lys Ser Thr Gln Leu Asp Tyr Val Asp 100 105 110
Leu Tyr Ile Ile His Phe Pro Met Ala Leu Gln Pro Gly Asp Ile Phe 115
120 125 Phe Pro Arg Asp Glu His Gly Lys Leu Leu Phe Glu Thr Val Asp
Ile 130 135 140 Cys Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp Ala
Gly Leu Ala 145 150 155 160 Lys Ser Ile Gly Val Ser Asn Phe Asn Cys
Arg Gln Leu Glu Arg Ile 165 170 175 Leu Asn Lys Pro Gly Leu Lys Tyr
Lys Pro Val Cys Asn Gln Val Glu 180 185 190 Cys His Leu Tyr Leu Asn
Gln Ser Lys Met Leu Asp Tyr Cys Lys Ser 195 200 205 Lys Asp Ile Ile
Leu Val Ser Tyr Cys Thr Leu Gly Ser Ser Arg Asp 210 215 220 Lys Thr
Trp Val Asp Gln Lys Ser Pro Val Leu Leu Asp Asp Pro Val 225 230 235
240 Leu Cys Ala Ile Ala Lys Lys Tyr Lys Gln Thr Pro Ala Leu Val Ala
245 250 255 Leu Arg Tyr Gln Leu Gln Arg Gly Val Val Pro Leu Ile Arg
Ser Phe 260 265 270 Asn Ala Lys Arg Ile Lys Glu Leu Thr Gln Val Phe
Glu Phe Gln Leu 275 280 285 Ala Ser Glu Asp Met Lys Ala Leu Asp Gly
Leu Asn Arg Asn Phe Arg 290 295 300 Tyr Asn Asn Ala Lys Tyr Phe Asp
Asp His Pro Asn His Pro Phe Thr 305 310 315 320 Asp Glu <210>
SEQ ID NO 9 <211> LENGTH: 26 <212> TYPE: DNA
<213> ORGANISM: Artificial <220> FEATURE: <223>
OTHER INFORMATION: PCR Primer <400> SEQUENCE: 9 cgtcgtcatg
gtcgcccgtc gcgagg 26 <210> SEQ ID NO 10 <211> LENGTH:
26 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 10 cctcgcgacg ggcgaccatg acgacg 26
<210> SEQ ID NO 11 <211> LENGTH: 26 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 11
cgtcgtcatg gtcagccgtc gcgagg 26 <210> SEQ ID NO 12
<211> LENGTH: 26 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 12 cctcgcgacg gctgaccatg acgacg 26
<210> SEQ ID NO 13 <211> LENGTH: 47 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 13
gcgaattcgt gaaaggagat atacatgaac ctgagggaga agtacgg 47 <210>
SEQ ID NO 14 <211> LENGTH: 1206 <212> TYPE: DNA
<213> ORGANISM: Artificial <220> FEATURE: <223>
OTHER INFORMATION: Formate Dehydrogenase Mutant <220>
FEATURE: <221> NAME/KEY: CDS <222> LOCATION:
(1)..(1206) <400> SEQUENCE: 14 atg gca aag gtc ctg tgc gtt
ctt tac gat gat ccg gtc gac ggc tac 48 Met Ala Lys Val Leu Cys Val
Leu Tyr Asp Asp Pro Val Asp Gly Tyr 1 5 10 15 ccg aag acc tat gcc
cgc gac gat ctt ccg aag atc gac cac tat ccg 96 Pro Lys Thr Tyr Ala
Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 20 25 30 ggc ggc cag
atc ttg ccg acg ccg aag gcc atc gac ttc acg ccc ggg 144 Gly Gly Gln
Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 35 40 45 cag
ttg ctc ggc tcc gtc tcc ggc gag ctc ggc ctg cgc gaa tat ctc 192 Gln
Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55
60 gaa tcc aac ggc cac acc ctg gtc gtg acc tcc gac aag gac ggc ccc
240 Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro
65 70 75 80 gac tcg gtg ttc gag cgc gag ctg gtc gat gcg gat gtc gtc
atc tcc 288 Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val
Ile Ser 85 90 95 cag ccc ttc tgg ccg gcc tat ctg acg ccc gag cgc
atc gcc aag gcc 336 Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg
Ile Ala Lys Ala 100 105 110 aag aac ctg aag ctc gcg ctc acc gcc ggc
atc ggt tcc gac cac gtc 384 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly
Ile Gly Ser Asp His Val 115 120 125 gat ctt cag tcg gct atc gac cgc
aac gtc acc gtg gcg gaa gtc acc 432 Asp Leu Gln Ser Ala Ile Asp Arg
Asn Val Thr Val Ala Glu Val Thr 130 135 140 tac tgc aac tcg atc agc
gtc gcc gag cat gtg gtg atg atg atc ctg 480 Tyr Cys Asn Ser Ile Ser
Val Ala Glu His Val Val Met Met Ile Leu 145 150 155 160 tcg ctg gtg
cgc aac tat ctg ccc tcg cac gaa tgg gcg cgg aag ggc 528 Ser Leu Val
Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 165 170 175 ggc
tgg aac atc gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg 576 Gly
Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 180 185
190 atg cat gtc ggc acc gtg gcc gcc ggc cgc atc ggt ctc gcg gtg ctg
624 Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu
195 200 205 cgc cgt ctg gcg ccg ttc gac gtg cac ctg cac tac acc ggc
cgt cac 672 Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly
Arg His 210 215 220 cgc ctg ccg gaa tcg gtc gag aag gag ctc aac ctc
acc tgg cac gcg 720 Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu
Thr Trp His Ala 225 230 235 240 acc cgc gag gac atg tat ccg gtt tgc
gac gtg gtg acg ctg aac tgc 768 Thr Arg Glu Asp Met Tyr Pro Val Cys
Asp Val Val Thr Leu Asn Cys 245 250 255 ccg ctg cac ccc gaa acc gag
cac atg atc aat gac gag acg ctg aag 816 Pro Leu His Pro Glu Thr Glu
His Met Ile Asn Asp Glu Thr Leu Lys 260 265 270 ctg ttc aag cgt ggc
gcc tac atc gtc aac acc gcc cgc ggc aag ctg 864 Leu Phe Lys Arg Gly
Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 275 280 285 tgc gac cgc
gat gcc gtg gca cgt gcg ctc gaa tcc ggc cgg ctg gcc 912 Cys Asp Arg
Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 290 295 300 ggc
tat gcc ggc gac gtg tgg ttc ccg cag ccg gcg ccg aag gac cac 960 Gly
Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 305 310
315 320 ccc tgg cgg acg atg ccc tat aac ggc atg acc ccg cac atc tcc
ggc 1008 Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile
Ser Gly 325 330 335 acc acg ctg acc gcg cag gcg cgt tat gcg gcg ggc
acc cgc gag atc 1056 Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala
Gly Thr Arg Glu Ile 340 345 350 ctg gag tgc ttc ttc gag ggc cgt ccg
atc cgc gac gaa tac ctc atc 1104 Leu Glu Cys Phe Phe Glu Gly Arg
Pro Ile Arg Asp Glu Tyr Leu Ile 355 360 365 gtg cag ggc ggc gct ctt
gcc ggc acc ggc gcg cat tcc tac tcg aag 1152 Val Gln Gly Gly Ala
Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 370 375 380 ggc aat gcc
acc ggc ggt tcg gaa gag gcc gcc aag ttc aag aag gcg 1200 Gly Asn
Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 385 390 395
400 gtc tga 1206 Val <210> SEQ ID NO 15 <211> LENGTH:
401 <212> TYPE: PRT <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: Synthetic
Construct <400> SEQUENCE: 15 Met Ala Lys Val Leu Cys Val Leu
Tyr Asp Asp Pro Val Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg
Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile
Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu
Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60
Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65
70 75 80 Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val
Ile Ser 85 90 95 Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg
Ile Ala Lys Ala 100 105 110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly
Ile Gly Ser Asp His Val 115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg
Asn Val Thr Val Ala Glu Val Thr 130 135 140 Tyr Cys Asn Ser Ile Ser
Val Ala Glu His Val Val Met Met Ile Leu 145 150 155 160 Ser Leu Val
Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 165 170 175 Gly
Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 180 185
190 Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu
195 200 205 Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly
Arg His 210 215 220 Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu
Thr Trp His Ala 225 230 235 240 Thr Arg Glu Asp Met Tyr Pro Val Cys
Asp Val Val Thr Leu Asn Cys 245 250 255 Pro Leu His Pro Glu Thr Glu
His Met Ile Asn Asp Glu Thr Leu Lys 260 265 270 Leu Phe Lys Arg Gly
Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 275 280 285 Cys Asp Arg
Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 290 295 300 Gly
Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 305 310
315 320 Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser
Gly 325 330 335 Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr
Arg Glu Ile 340 345 350 Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg
Asp Glu Tyr Leu Ile 355 360 365 Val Gln Gly Gly Ala Leu Ala Gly Thr
Gly Ala His Ser Tyr Ser Lys 370 375 380 Gly Asn Ala Thr Gly Gly Ser
Glu Glu Ala Ala Lys Phe Lys Lys Ala 385 390 395 400 Val <210>
SEQ ID NO 16 <211> LENGTH: 43 <212> TYPE: DNA
<213> ORGANISM: Artificial <220> FEATURE: <223>
OTHER INFORMATION: PCR Primer <400> SEQUENCE: 16 cccaagctta
aggagatata catgtccatc atcgtgataa gcg 43 <210> SEQ ID NO 17
<211> LENGTH: 34 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 17 ataagaatgc ggccgctcag aactgtgtcg
ggcg 34 <210> SEQ ID NO 18 <211> LENGTH: 7404
<212> TYPE: DNA <213> ORGANISM: Artificial <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pET21a FDH D221G
7beta-HSDH <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (5208)..(6422) <223> OTHER INFORMATION:
FDH D221G <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (6435)..(7226) <223> OTHER INFORMATION:
7beta-HSDH <400> SEQUENCE: 18 tggcgaatgg gacgcgccct
gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60 cagcgtgacc
gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
180 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
gtgatggttc 240 acgtagtggg ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt 300 ctttaatagt ggactcttgt tccaaactgg
aacaacactc aaccctatct cggtctattc 360 ttttgattta taagggattt
tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420 acaaaaattt
aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
540 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
ggaagagtat 600 gagtattcaa catttccgtg tcgcccttat tccctttttt
gcggcatttt gccttcctgt 660 ttttgctcac ccagaaacgc tggtgaaagt
aaaagatgct gaagatcagt tgggtgcacg 720 agtgggttac atcgaactgg
atctcaacag cggtaagatc cttgagagtt ttcgccccga 780 agaacgtttt
ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 840
tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
900 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
gagaattatg 960 cagtgctgcc ataaccatga gtgataacac tgcggccaac
ttacttctga caacgatcgg 1020 aggaccgaag gagctaaccg cttttttgca
caacatgggg gatcatgtaa ctcgccttga 1080 tcgttgggaa ccggagctga
atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1140 tgcagcaatg
gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1200
ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
1260 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
gtgggtctcg 1320 cggtatcatt gcagcactgg ggccagatgg taagccctcc
cgtatcgtag ttatctacac 1380 gacggggagt caggcaacta tggatgaacg
aaatagacag atcgctgaga taggtgcctc 1440 actgattaag cattggtaac
tgtcagacca agtttactca tatatacttt agattgattt 1500 aaaacttcat
ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1560
caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
1620 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
caaaaaaacc 1680 accgctacca gcggtggttt gtttgccgga tcaagagcta
ccaactcttt ttccgaaggt 1740 aactggcttc agcagagcgc agataccaaa
tactgtcctt ctagtgtagc cgtagttagg 1800 ccaccacttc aagaactctg
tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1860 agtggctgct
gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1920
accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
1980 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
gcgccacgct 2040 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
agggtcggaa caggagagcg 2100 cacgagggag cttccagggg gaaacgcctg
gtatctttat agtcctgtcg ggtttcgcca 2160 cctctgactt gagcgtcgat
ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2220 cgccagcaac
gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2280
ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
2340 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
aagcggaaga 2400 gcgcctgatg cggtattttc tccttacgca tctgtgcggt
atttcacacc gcatatatgg 2460 tgcactctca gtacaatctg ctctgatgcc
gcatagttaa gccagtatac actccgctat 2520 cgctacgtga ctgggtcatg
gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2580 gacgggcttg
tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2640
gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
2700 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
tccagctcgt 2760 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
gcgggccatg ttaagggcgg 2820 ttttttcctg tttggtcact gatgcctccg
tgtaaggggg atttctgttc atgggggtaa 2880 tgataccgat gaaacgagag
aggatgctca cgatacgggt tactgatgat gaacatgccc 2940 ggttactgga
acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3000
aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
3060 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
gacttccgcg 3120 tttccagact ttacgaaaca cggaaaccga agaccattca
tgttgttgct caggtcgcag 3180 acgttttgca gcagcagtcg cttcacgttc
gctcgcgtat cggtgattca ttctgctaac 3240 cagtaaggca accccgccag
cctagccggg tcctcaacga caggagcacg atcatgcgca 3300 cccgtggggc
cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3360
gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
3420 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
agcgctgccg 3480 gcacctgtcc tacgagttgc atgataaaga agacagtcat
aagtgcggcg acgatagtca 3540 tgccccgcgc ccaccggaag gagctgactg
ggttgaaggc tctcaagggc atcggtcgag 3600 atcccggtgc ctaatgagtg
agctaactta cattaattgc gttgcgctca ctgcccgctt 3660 tccagtcggg
aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3720
gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
3780 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
gctggtttgc 3840 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
ggatataaca tgagctgtct 3900 tcggtatcgt cgtatcccac taccgagata
tccgcaccaa cgcgcagccc ggactcggta 3960 atggcgcgca ttgcgcccag
cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4020 atgccctcat
tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4080
tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
4140 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
ctggtgaccc 4200 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
catgggagaa aataatactg 4260 ttgatgggtg tctggtcaga gacatcaaga
aataacgccg gaacattagt gcaggcagct 4320 tccacagcaa tggcatcctg
gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4380 tgcgcgagaa
gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4440
gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
4500 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
ctgtttgccc 4560 gccagttgtt gtgccacgcg gttgggaatg taattcagct
ccgccatcgc cgcttccact 4620 ttttcccgcg ttttcgcaga aacgtggctg
gcctggttca ccacgcggga aacggtctga 4680 taagagacac cggcatactc
tgcgacatcg tataacgtta ctggtttcac attcaccacc 4740 ctgaattgac
tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4800
atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
4860 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
aggagatggc 4920 gcccaacagt cccccggcca cggggcctgc caccataccc
acgccgaaac aagcgctcat 4980 gagcccgaag tggcgagccc gatcttcccc
atcggtgatg tcggcgatat aggcgccagc 5040 aaccgcacct gtggcgccgg
tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5100 ctcgatcccg
cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 5160
cctctagaaa taattttgtt taactttaag aaggagatat acatatg atg gca aag
5216 Met Ala Lys 1 gtc ctg tgc gtt ctt tac gat gat ccg gtc gac ggc
tac ccg aag acc 5264 Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp
Gly Tyr Pro Lys Thr 5 10 15 tat gcc cgc gac gat ctt ccg aag atc gac
cac tat ccg ggc ggc cag 5312 Tyr Ala Arg Asp Asp Leu Pro Lys Ile
Asp His Tyr Pro Gly Gly Gln 20 25 30 35 atc ttg ccg acg ccg aag gcc
atc gac ttc acg ccc ggg cag ttg ctc 5360 Ile Leu Pro Thr Pro Lys
Ala Ile Asp Phe Thr Pro Gly Gln Leu Leu 40 45 50 ggc tcc gtc tcc
ggc gag ctc ggc ctg cgc gaa tat ctc gaa tcc aac 5408 Gly Ser Val
Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu Glu Ser Asn 55 60 65 ggc
cac acc ctg gtc gtg acc tcc gac aag gac ggc ccc gac tcg gtg 5456
Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro Asp Ser Val 70
75 80 ttc gag cgc gag ctg gtc gat gcg gat gtc gtc atc tcc cag ccc
ttc 5504 Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser Gln
Pro Phe 85 90 95 tgg ccg gcc tat ctg acg ccc gag cgc atc gcc aag
gcc aag aac ctg 5552 Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala
Lys Ala Lys Asn Leu 100 105 110 115 aag ctc gcg ctc acc gcc ggc atc
ggt tcc gac cac gtc gat ctt cag 5600 Lys Leu Ala Leu Thr Ala Gly
Ile Gly Ser Asp His Val Asp Leu Gln 120 125 130 tcg gct atc gac cgc
aac gtc acc gtg gcg gaa gtc acc tac tgc aac 5648 Ser Ala Ile Asp
Arg Asn Val Thr Val Ala Glu Val Thr Tyr Cys Asn 135 140 145 tcg atc
agc gtc gcc gag cat gtg gtg atg atg atc ctg tcg ctg gtg 5696 Ser
Ile Ser Val Ala Glu His Val Val Met Met Ile Leu Ser Leu Val 150 155
160 cgc aac tat ctg ccc tcg cac gaa tgg gcg cgg aag ggc ggc tgg aac
5744 Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly Gly Trp
Asn 165 170 175 atc gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg
atg cat gtc 5792 Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu
Ala Met His Val 180 185 190 195 ggc acc gtg gcc gcc ggc cgc atc ggt
ctc gcg gtg ctg cgc cgt ctg 5840 Gly Thr Val Ala Ala Gly Arg Ile
Gly Leu Ala Val Leu Arg Arg Leu 200 205 210 gcg ccg ttc gac gtg cac
ctg cac tac acc ggc cgt cac cgc ctg ccg 5888 Ala Pro Phe Asp Val
His Leu His Tyr Thr Gly Arg His Arg Leu Pro 215 220 225 gaa tcg gtc
gag aag gag ctc aac ctc acc tgg cac gcg acc cgc gag 5936 Glu Ser
Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala Thr Arg Glu 230 235 240
gac atg tat ccg gtt tgc gac gtg gtg acg ctg aac tgc ccg ctg cac
5984 Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys Pro Leu
His 245 250 255 ccc gaa acc gag cac atg atc aat gac gag acg ctg aag
ctg ttc aag 6032 Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu
Lys Leu Phe Lys 260 265 270 275 cgt ggc gcc tac atc gtc aac acc gcc
cgc ggc aag ctg tgc gac cgc 6080 Arg Gly Ala Tyr Ile Val Asn Thr
Ala Arg Gly Lys Leu Cys Asp Arg 280 285 290 gat gcc gtg gca cgt gcg
ctc gaa tcc ggc cgg ctg gcc ggc tat gcc 6128 Asp Ala Val Ala Arg
Ala Leu Glu Ser Gly Arg Leu Ala Gly Tyr Ala 295 300 305 ggc gac gtg
tgg ttc ccg cag ccg gcg ccg aag gac cac ccc tgg cgg 6176 Gly Asp
Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His Pro Trp Arg 310 315 320
acg atg ccc tat aac ggc atg acc ccg cac atc tcc ggc acc acg ctg
6224 Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly Thr Thr
Leu 325 330 335 acc gcg cag gcg cgt tat gcg gcg ggc acc cgc gag atc
ctg gag tgc 6272 Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu
Ile Leu Glu Cys 340 345 350 355 ttc ttc gag ggc cgt ccg atc cgc gac
gaa tac ctc atc gtg cag ggc 6320 Phe Phe Glu Gly Arg Pro Ile Arg
Asp Glu Tyr Leu Ile Val Gln Gly 360 365 370 ggc gct ctt gcc ggc acc
ggc gcg cat tcc tac tcg aag ggc aat gcc 6368 Gly Ala Leu Ala Gly
Thr Gly Ala His Ser Tyr Ser Lys Gly Asn Ala 375 380 385 acc ggc ggt
tcg gaa gag gcc gcc aag ttc aag aag gcg gct gag aat 6416 Thr Gly
Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala Ala Glu Asn 390 395 400
tcg tga aaggagatat ac atg aac ctg agg gag aag tac ggt gag tgg ggc
6467 Ser Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly 405 410 415
ctg atc ctg ggc gcg acc gag ggc gtc ggc aag gcg ttc tgc gag aag
6515 Leu Ile Leu Gly Ala Thr Glu Gly Val Gly Lys Ala Phe Cys Glu
Lys 420 425 430 atc gcc gcc ggc ggc atg aac gtc gtc atg gtc ggc cgt
cgc gag gag 6563 Ile Ala Ala Gly Gly Met Asn Val Val Met Val Gly
Arg Arg Glu Glu 435 440 445 aag ctg aac gtg ctc gca ggc gag atc cgc
gag acc tac ggc gtg gag 6611 Lys Leu Asn Val Leu Ala Gly Glu Ile
Arg Glu Thr Tyr Gly Val Glu 450 455 460 acc aag gtc gtg cgc gcc gac
ttt agc cag ccc ggc gct gcc gag acc 6659 Thr Lys Val Val Arg Ala
Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr 465 470 475 gtc ttc gcc gcg
acc gag ggc ctg gac atg ggc ttc atg agc tac gtg 6707 Val Phe Ala
Ala Thr Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val 480 485 490 495
gcc tgc ctg cac agc ttc ggt aag atc cag gac acc ccc tgg gag aag
6755 Ala Cys Leu His Ser Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu
Lys 500 505 510 cac gag gcc atg atc aac gtc aac gtc gtg acc ttc ctc
aag tgc ttc 6803 His Glu Ala Met Ile Asn Val Asn Val Val Thr Phe
Leu Lys Cys Phe 515 520 525 cac cac tac atg cgg atc ttt gcc gcc cag
gac cgc ggc gcc gtg atc 6851 His His Tyr Met Arg Ile Phe Ala Ala
Gln Asp Arg Gly Ala Val Ile 530 535 540 aac gtc tcg tcg atg acc ggc
atc agc tcc agc ccc tgg aac ggc cag 6899 Asn Val Ser Ser Met Thr
Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln 545 550 555 tac ggc gcg ggc
aag gcc ttc atc ctc aag atg acc gag gcc gtg gcc 6947 Tyr Gly Ala
Gly Lys Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala 560 565 570 575
tgc gag tgc gag ggc acc ggc gtc gac gtc gag gtc atc acc ctc ggc
6995 Cys Glu Cys Glu Gly Thr Gly Val Asp Val Glu Val Ile Thr Leu
Gly 580 585 590 acc acc cta acc ccc agc ctg ctg tcc aac ctc ccc ggc
ggc ccg cag 7043 Thr Thr Leu Thr Pro Ser Leu Leu Ser Asn Leu Pro
Gly Gly Pro Gln 595 600 605 ggc gag gcc gtc atg aag atc gcc ctc acc
ccc gag gag tgc gtt gac 7091 Gly Glu Ala Val Met Lys Ile Ala Leu
Thr Pro Glu Glu Cys Val Asp 610 615 620 gag gcc ttt gag aag ctg ggt
aag gag ctc tcc gtc atc gcc ggc cag 7139 Glu Ala Phe Glu Lys Leu
Gly Lys Glu Leu Ser Val Ile Ala Gly Gln 625 630 635 cgc aac aag gac
tcc gtc cac gac tgg aag gca aac cac acc gag gac 7187 Arg Asn Lys
Asp Ser Val His Asp Trp Lys Ala Asn His Thr Glu Asp 640 645 650 655
gag tac atc cgc tac atg ggg tcg ttc tac cgc gac tag aagcttgcgg 7236
Glu Tyr Ile Arg Tyr Met Gly Ser Phe Tyr Arg Asp 660 665 ccgcactcga
gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg 7296
aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
7356 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat 7404
<210> SEQ ID NO 19 <211> LENGTH: 404 <212> TYPE:
PRT <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: Synthetic Construct <400>
SEQUENCE: 19 Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val
Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys
Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile Leu Pro Thr Pro Lys
Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu Leu Gly Ser Val Ser
Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 Glu Ser Asn Gly His
Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80 Asp Ser Val
Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85 90 95 Gln
Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 100 105
110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val
115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu
Val Thr 130 135 140 Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val
Met Met Ile Leu 145 150 155 160 Ser Leu Val Arg Asn Tyr Leu Pro Ser
His Glu Trp Ala Arg Lys Gly 165 170 175 Gly Trp Asn Ile Ala Asp Cys
Val Ser His Ala Tyr Asp Leu Glu Ala 180 185 190 Met His Val Gly Thr
Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 195 200 205 Arg Arg Leu
Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 210 215 220 Arg
Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 225 230
235 240 Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn
Cys 245 250 255 Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu
Thr Leu Lys 260 265 270 Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr
Ala Arg Gly Lys Leu 275 280 285 Cys Asp Arg Asp Ala Val Ala Arg Ala
Leu Glu Ser Gly Arg Leu Ala 290 295 300 Gly Tyr Ala Gly Asp Val Trp
Phe Pro Gln Pro Ala Pro Lys Asp His 305 310 315 320 Pro Trp Arg Thr
Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 325 330 335 Thr Thr
Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 340 345 350
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355
360 365 Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser
Lys 370 375 380 Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe
Lys Lys Ala 385 390 395 400 Ala Glu Asn Ser <210> SEQ ID NO
20 <211> LENGTH: 263 <212> TYPE: PRT <213>
ORGANISM: Artificial <220> FEATURE: <223> OTHER
INFORMATION: Synthetic Construct <400> SEQUENCE: 20 Met Asn
Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20
25 30 Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val
Leu 35 40 45 Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys
Val Val Arg 50 55 60 Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr
Val Phe Ala Ala Thr 65 70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser
Tyr Val Ala Cys Leu His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr
Pro Trp Glu Lys His Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val
Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala
Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr
Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150
155 160 Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu
Gly 165 170 175 Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr
Leu Thr Pro 180 185 190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln
Gly Glu Ala Val Met 195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys
Val Asp Glu Ala Phe Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val
Ile Ala Gly Gln Arg Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp
Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly
Ser Phe Tyr Arg Asp 260 <210> SEQ ID NO 21 <211>
LENGTH: 4302 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: Plasmid
pCOLA(mod) 3alpha-HSDH <220> FEATURE: <221> NAME/KEY:
CDS <222> LOCATION: (303)..(1076) <223> OTHER
INFORMATION: 3alpha-HSDH <400> SEQUENCE: 21 ggggaattgt
gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 60
gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
120 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc
gaacagaaag 180 taatcgtatt gtacacggcc gcataatcga aattaatacg
actcactata ggggaattgt 240 gagcggataa caattcccca tcttagtata
ttagttaagt ataagaagga gatatacata 300 tg atg tcc atc atc gtg ata agc
ggc tgc gcc acc ggc att ggt gcc 347 Met Ser Ile Ile Val Ile Ser Gly
Cys Ala Thr Gly Ile Gly Ala 1 5 10 15 gct acg cgc aag gtc ctg gag
gcg gcc ggt cac cag atc gta ggc atc 395 Ala Thr Arg Lys Val Leu Glu
Ala Ala Gly His Gln Ile Val Gly Ile 20 25 30 gat ata cgc gat gcg
gaa gtg att gcc gat ctc tcg acg gcc gaa ggt 443 Asp Ile Arg Asp Ala
Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly 35 40 45 cga aag cag
gcg att gcc gat gta ctg gcg aag tgc agc aag ggc atg 491 Arg Lys Gln
Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met 50 55 60 gac
ggc ctg gtg ctg tgc gcc ggc ctg gga ccg cag acc aag gtg ctt 539 Asp
Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu 65 70
75 ggc aat gtg gtt tcg gtc aat tat ttt ggc gcg acc gag ctg atg gat
587 Gly Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp
80 85 90 95 gcc ttt ttg cca gcg ctg aaa aaa ggc cat cag ccc gca gcc
gtc gtc 635 Ala Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala
Val Val 100 105 110 atc tcg tcc gtg gct tcc gcg cat ctg gct ttt gac
aag aac cca ctg 683 Ile Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp
Lys Asn Pro Leu 115 120 125 gcg ctg gca ctg gaa gcc ggc gag gaa gcc
aag gcc cgc gcc att gtc 731 Ala Leu Ala Leu Glu Ala Gly Glu Glu Ala
Lys Ala Arg Ala Ile Val 130 135 140 gaa cat gcg gga gag cag ggc gga
aat ctg gcc tat gcg ggc agc aag 779 Glu His Ala Gly Glu Gln Gly Gly
Asn Leu Ala Tyr Ala Gly Ser Lys 145 150 155 aat gct ttg acg gtg gct
gtg cgc aaa cgc gcc gcc gcc tgg ggc gag 827 Asn Ala Leu Thr Val Ala
Val Arg Lys Arg Ala Ala Ala Trp Gly Glu 160 165 170 175 gct ggc gtg
cgc ctg aac acc atc gcc ccc ggt gca acc gag act ccc 875 Ala Gly Val
Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro 180 185 190 ttg
ctg cag gcg ggc ctg cag gac ccg cgc tat ggc gaa tcc att gcc 923 Leu
Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala 195 200
205 aag ttc gtt cct ccc atg ggc cgc cgt gcc gag ccg tcc gag atg gcg
971 Lys Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala
210 215 220 tcg gtc atc gcc ttt ttg atg agc ccg gcc gca agc tat gtg
cat ggc 1019 Ser Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr
Val His Gly 225 230 235 gcg cag atc gtc att gat ggc ggc att gat gcg
gtg atg cgc ccg aca 1067 Ala Gln Ile Val Ile Asp Gly Gly Ile Asp
Ala Val Met Arg Pro Thr 240 245 250 255 cag ttc tga gaattcgagc
tccgtcgaca agcttgcggc cgcactcgag 1116 Gln Phe caccaccacc accaccactg
agatccggct gctaacaaag cccgaaagga agctgagttg 1176 gctgctgcca
ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg 1236
aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta
1296 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
ctgccctgaa 1356 ccgacgacaa gctgacgacc gggtctccgc aagtggcact
tttcggggaa atgtgcgcgg 1416 aacccctatt tgtttatttt tctaaataca
ttcaaatatg tatccgctca tgaattaatt 1476 cttacgcccc gccctgccac
tcatcgcagt actgttgtaa ttcattaagc attctgccga 1536 catggaagcc
atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt 1596
cgccttgcgt ataatatttg cctatggtga aaacgggggc gaagaagttg tccatattgg
1656 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
aaaaacatat 1716 tctcaataaa ccctttaggg aaataggcca ggttttcacc
gtaacacgcc acatcttgcg 1776 aatatatgtg tagaaactgc cggaaatcgt
cgtggtattc actccagagc gatgaaaacg 1836 tttcagtttg ctcatggaaa
acggtgtaac aagggtgaac actatcccat atcaccagct 1896 caccgtcttt
cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt 1956
gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
2016 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
gcctcaaaat 2076 gttctttacg atgccattgg gatatatcaa cggtggtata
tccagtgatt tttttctcca 2136 tactcttcct ttttcaatat tattgaagca
tttatcaggg ttattgtctc atgagcggat 2196 acatatttga atgtatttag
aaaaataaac aaataggcat gctagcgcag aaacgtccta 2256 gaagatgcca
ggaggatact tagcagagag acaataaggc cggagcgaag ccgtttttcc 2316
ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag tggtggcgaa
2376 acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
ttgcgctctc 2436 ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt
tacccaaatc accacgtccc 2496 gttccgtgta gacagttcgc tccaagctgg
gctgtgtgca agaacccccc gttcagcccg 2556 actgctgcgc cttatccggt
aactatcatc ttgagtccaa cccggaaaga cacgacaaaa 2616 cgccactggc
agcagccatt ggtaactgag aattagtgga tttagatatc gagagtcttg 2676
aagtggtggc ctaacagagg ctacactgaa aggacagtat ttggtatctg cgctccacta
2736 aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
aaccgcctcc 2796 ccaggcggtt ttttcgttta cagagcagga gattacgacg
atcgtaaaag gatctcaaga 2856 agatccttta cggattcccg acaccatcac
tctagatttc agtgcaattt atctcttcaa 2916 atgtagcacc tgaagtcagc
cccatacgat ataagttgta attctcatgt tagtcatgcc 2976 ccgcgcccac
cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc 3036
cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
3096 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
ggagaggcgg 3156 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
tgagacgggc aacagctgat 3216 tgcccttcac cgcctggccc tgagagagtt
gcagcaagcg gtccacgctg gtttgcccca 3276 gcaggcgaaa atcctgtttg
atggtggtta acggcgggat ataacatgag ctgtcttcgg 3336 tatcgtcgta
tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg 3396
cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
3456 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
tcgccttccc 3516 gttccgctat cggctgaatt tgattgcgag tgagatattt
atgccagcca gccagacgca 3576 gacgcgccga gacagaactt aatgggcccg
ctaacagcgc gatttgctgg tgacccaatg 3636 cgaccagatg ctccacgccc
agtcgcgtac cgtcttcatg ggagaaaata atactgttga 3696 tgggtgtctg
gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca 3756
cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
3816 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
accatcgaca 3876 ccaccacgct ggcacccagt tgatcggcgc gagatttaat
cgccgcgaca atttgcgacg 3936 gcgcgtgcag ggccagactg gaggtggcaa
cgccaatcag caacgactgt ttgcccgcca 3996 gttgttgtgc cacgcggttg
ggaatgtaat tcagctccgc catcgccgct tccacttttt 4056 cccgcgtttt
cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag 4116
agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
4176 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
cattcgatgg 4236 tgtccgggat ctcgacgctc tcccttatgc gactcctgca
ttaggaaatt aatacgactc 4296 actata 4302 <210> SEQ ID NO 22
<211> LENGTH: 257 <212> TYPE: PRT <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION:
Synthetic Construct <400> SEQUENCE: 22 Met Ser Ile Ile Val
Ile Ser Gly Cys Ala Thr Gly Ile Gly Ala Ala 1 5 10 15 Thr Arg Lys
Val Leu Glu Ala Ala Gly His Gln Ile Val Gly Ile Asp 20 25 30 Ile
Arg Asp Ala Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg 35 40
45 Lys Gln Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp
50 55 60 Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val
Leu Gly 65 70 75 80 Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu
Leu Met Asp Ala 85 90 95 Phe Leu Pro Ala Leu Lys Lys Gly His Gln
Pro Ala Ala Val Val Ile 100 105 110 Ser Ser Val Ala Ser Ala His Leu
Ala Phe Asp Lys Asn Pro Leu Ala 115 120 125 Leu Ala Leu Glu Ala Gly
Glu Glu Ala Lys Ala Arg Ala Ile Val Glu 130 135 140 His Ala Gly Glu
Gln Gly Gly Asn Leu Ala Tyr Ala Gly Ser Lys Asn 145 150 155 160 Ala
Leu Thr Val Ala Val Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala 165 170
175 Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu
180 185 190 Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile
Ala Lys 195 200 205 Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser
Glu Met Ala Ser 210 215 220 Val Ile Ala Phe Leu Met Ser Pro Ala Ala
Ser Tyr Val His Gly Ala 225 230 235 240 Gln Ile Val Ile Asp Gly Gly
Ile Asp Ala Val Met Arg Pro Thr Gln 245 250 255 Phe <210> SEQ
ID NO 23 <211> LENGTH: 29 <212> TYPE: DNA <213>
ORGANISM: Artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer <400> SEQUENCE: 23 cgatcatatg
gcaaaggtcc tgtgcgttc 29 <210> SEQ ID NO 24 <211>
LENGTH: 29 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 24 gctagaattc tcagccgcct tcttgaact 29
<210> SEQ ID NO 25 <211> LENGTH: 768 <212> TYPE:
DNA <213> ORGANISM: Escherichia coli <220> FEATURE:
<221> NAME/KEY: CDS <222> LOCATION: (1)..(768)
<400> SEQUENCE: 25 gtg ttt aat tct gac aac ctg aga ctc gac
gga aaa tgc gcc atc atc 48 Val Phe Asn Ser Asp Asn Leu Arg Leu Asp
Gly Lys Cys Ala Ile Ile 1 5 10 15 aca ggt gcg ggt gca ggt att ggt
aaa gaa atc gcc att aca ttc gcg 96 Thr Gly Ala Gly Ala Gly Ile Gly
Lys Glu Ile Ala Ile Thr Phe Ala 20 25 30 aca gct ggc gca tct gtg
gtg gtc agt gat att aac gcc gac gca gct 144 Thr Ala Gly Ala Ser Val
Val Val Ser Asp Ile Asn Ala Asp Ala Ala 35 40 45 aac cat gtt gta
gac gaa att caa caa ctg ggt ggt cag gca ttt gcc 192 Asn His Val Val
Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 50 55 60 tgc cgt
tgt gat att act tcc gaa cag gaa ctc tct gca ctg gca gac 240 Cys Arg
Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 65 70 75 80
ttt gct atc agt aag ctg ggt aaa gtt gat att ctg gtt aac aac gcc 288
Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 85
90 95 ggt ggc ggt gga cct aaa ccg ttt gat atg cca atg gcg gat ttt
cgc 336 Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe
Arg 100 105 110 cgt gct tat gaa ctg aat gtg ttt tct ttt ttc cat ctg
tca caa ctt 384 Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu
Ser Gln Leu 115 120 125 gtt gcg cca gaa atg gaa aaa aat ggc ggt ggc
gtt att ctg acc atc 432 Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly
Val Ile Leu Thr Ile 130 135 140 act tct atg gcg gca gaa aat aaa aat
ata aac atg act tcc tat gca 480 Thr Ser Met Ala Ala Glu Asn Lys Asn
Ile Asn Met Thr Ser Tyr Ala 145 150 155 160 tca tct aaa gct gcg gcc
agt cat ctg gtc aga aat atg gcg ttt gac 528 Ser Ser Lys Ala Ala Ala
Ser His Leu Val Arg Asn Met Ala Phe Asp 165 170 175 cta ggt gaa aaa
aat att cgg gta aat ggc att gcg ccg ggg gca ata 576 Leu Gly Glu Lys
Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 180 185 190 tta acc
gat gcc ctg aaa tcc gtt att aca cca gaa att gaa caa aaa 624 Leu Thr
Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 195 200 205
atg tta cag cac acg ccg atc aga cgt ctg ggc caa ccg caa gat att 672
Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 210
215 220 gct aac gca gcg ctg ttc ctt tgc tcg cct gct gcg agc tgg gta
agc 720 Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val
Ser 225 230 235 240 gga caa att ctc acc gtc tcc ggt ggt ggg gta cag
gag ctc aat taa 768 Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln
Glu Leu Asn 245 250 255 <210> SEQ ID NO 26 <211>
LENGTH: 255 <212> TYPE: PRT <213> ORGANISM: Escherichia
coli <400> SEQUENCE: 26 Val Phe Asn Ser Asp Asn Leu Arg Leu
Asp Gly Lys Cys Ala Ile Ile 1 5 10 15 Thr Gly Ala Gly Ala Gly Ile
Gly Lys Glu Ile Ala Ile Thr Phe Ala 20 25 30 Thr Ala Gly Ala Ser
Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala 35 40 45 Asn His Val
Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 50 55 60 Cys
Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 65 70
75 80 Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn
Ala 85 90 95 Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala
Asp Phe Arg 100 105 110 Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe
His Leu Ser Gln Leu 115 120 125 Val Ala Pro Glu Met Glu Lys Asn Gly
Gly Gly Val Ile Leu Thr Ile 130 135 140 Thr Ser Met Ala Ala Glu Asn
Lys Asn Ile Asn Met Thr Ser Tyr Ala 145 150 155 160 Ser Ser Lys Ala
Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 165 170 175 Leu Gly
Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 180 185 190
Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 195
200 205 Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp
Ile 210 215 220 Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser
Trp Val Ser 225 230 235 240 Gly Gln Ile Leu Thr Val Ser Gly Gly Gly
Val Gln Glu Leu Asn 245 250 255 <210> SEQ ID NO 27
<211> LENGTH: 53 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 27 aaaaaagctt ataattatcc ttataggacg
tcatggtgcg cccagatagg gtg 53 <210> SEQ ID NO 28 <211>
LENGTH: 60 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 28 cagattgtac aaatgtggtg ataacagata
agtcgtcatg tttaacttac ctttctttgt 60 <210> SEQ ID NO 29
<211> LENGTH: 49 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 29 tgaacgcaag tttctaattt cggtttccta
tcgatagagg aaagtgtct 49 <210> SEQ ID NO 30 <211>
LENGTH: 29 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 30 cctgcactac accggccgtc accgcctgc 29
<210> SEQ ID NO 31 <211> LENGTH: 23 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 31
gctcgaattc tcagaccgcc ttc 23 <210> SEQ ID NO 32 <211>
LENGTH: 27 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 32 ttaattgagc tcctgtaccc caccacc 27
<210> SEQ ID NO 33 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 33
gtgtttaatt ctgacaacct gagactcgac 30 <210> SEQ ID NO 34
<211> LENGTH: 1266 <212> TYPE: DNA <213>
ORGANISM: Artificial <220> FEATURE: <223> OTHER
INFORMATION: FDH D221G Mutant with His-Tag <220> FEATURE:
<221> NAME/KEY: CDS <222> LOCATION: (1)..(1266)
<400> SEQUENCE: 34 atg gca aag gtc ctg tgc gtt ctt tac gat
gat ccg gtc gac ggc tac 48 Met Ala Lys Val Leu Cys Val Leu Tyr Asp
Asp Pro Val Asp Gly Tyr 1 5 10 15 ccg aag acc tat gcc cgc gac gat
ctt ccg aag atc gac cac tat ccg 96 Pro Lys Thr Tyr Ala Arg Asp Asp
Leu Pro Lys Ile Asp His Tyr Pro 20 25 30 ggc ggc cag atc ttg ccg
acg ccg aag gcc atc gac ttc acg ccc ggg 144 Gly Gly Gln Ile Leu Pro
Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 35 40 45 cag ttg ctc ggc
tcc gtc tcc ggc gag ctc ggc ctg cgc gaa tat ctc 192 Gln Leu Leu Gly
Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 gaa tcc
aac ggc cac acc ctg gtc gtg acc tcc gac aag gac ggc ccc 240 Glu Ser
Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80
gac tcg gtg ttc gag cgc gag ctg gtc gat gcg gat gtc gtc atc tcc 288
Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85
90 95 cag ccc ttc tgg ccg gcc tat ctg acg ccc gag cgc atc gcc aag
gcc 336 Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys
Ala 100 105 110 aag aac ctg aag ctc gcg ctc acc gcc ggc atc ggt tcc
gac cac gtc 384 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser
Asp His Val 115 120 125 gat ctt cag tcg gct atc gac cgc aac gtc acc
gtg gcg gaa gtc acc 432 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr
Val Ala Glu Val Thr 130 135 140 tac tgc aac tcg atc agc gtc gcc gag
cat gtg gtg atg atg atc ctg 480 Tyr Cys Asn Ser Ile Ser Val Ala Glu
His Val Val Met Met Ile Leu 145 150 155 160 tcg ctg gtg cgc aac tat
ctg ccc tcg cac gaa tgg gcg cgg aag ggc 528 Ser Leu Val Arg Asn Tyr
Leu Pro Ser His Glu Trp Ala Arg Lys Gly 165 170 175 ggc tgg aac atc
gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg 576 Gly Trp Asn Ile
Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 180 185 190 atg cat
gtc ggc acc gtg gcc gcc ggc cgc atc ggt ctc gcg gtg ctg 624 Met His
Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 195 200 205
cgc cgt ctg gcg ccg ttc gac gtg cac ctg cac tac acc ggc cgt cac 672
Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 210
215 220 cgc ctg ccg gaa tcg gtc gag aag gag ctc aac ctc acc tgg cac
gcg 720 Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His
Ala 225 230 235 240 acc cgc gag gac atg tat ccg gtt tgc gac gtg gtg
acg ctg aac tgc 768 Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val
Thr Leu Asn Cys 245 250 255 ccg ctg cac ccc gaa acc gag cac atg atc
aat gac gag acg ctg aag 816 Pro Leu His Pro Glu Thr Glu His Met Ile
Asn Asp Glu Thr Leu Lys 260 265 270 ctg ttc aag cgt ggc gcc tac atc
gtc aac acc gcc cgc ggc aag ctg 864 Leu Phe Lys Arg Gly Ala Tyr Ile
Val Asn Thr Ala Arg Gly Lys Leu 275 280 285 tgc gac cgc gat gcc gtg
gca cgt gcg ctc gaa tcc ggc cgg ctg gcc 912 Cys Asp Arg Asp Ala Val
Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 290 295 300 ggc tat gcc ggc
gac gtg tgg ttc ccg cag ccg gcg ccg aag gac cac 960 Gly Tyr Ala Gly
Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 305 310 315 320 ccc
tgg cgg acg atg ccc tat aac ggc atg acc ccg cac atc tcc ggc 1008
Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 325
330 335 acc acg ctg acc gcg cag gcg cgt tat gcg gcg ggc acc cgc gag
atc 1056 Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg
Glu Ile 340 345 350 ctg gag tgc ttc ttc gag ggc cgt ccg atc cgc gac
gaa tac ctc atc 1104 Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg
Asp Glu Tyr Leu Ile 355 360 365 gtg cag ggc ggc gct ctt gcc ggc acc
ggc gcg cat tcc tac tcg aag 1152 Val Gln Gly Gly Ala Leu Ala Gly
Thr Gly Ala His Ser Tyr Ser Lys 370 375 380 ggc aat gcc acc ggc ggt
tcg gaa gag gcc gcc aag ttc aag aag gcg 1200 Gly Asn Ala Thr Gly
Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 385 390 395 400 gct gag
aat tcg agc tcc gtc gac aag ctt gcg gcc gca ctc gag cac 1248 Ala
Glu Asn Ser Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His 405 410
415 cac cac cac cac cac tga 1266 His His His His His 420
<210> SEQ ID NO 35 <211> LENGTH: 421 <212> TYPE:
PRT <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: Synthetic Construct <400>
SEQUENCE: 35 Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val
Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys
Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile Leu Pro Thr Pro Lys
Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu Leu Gly Ser Val Ser
Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 Glu Ser Asn Gly His
Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80 Asp Ser Val
Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85 90 95 Gln
Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 100 105
110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val
115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu
Val Thr 130 135 140 Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val
Met Met Ile Leu 145 150 155 160 Ser Leu Val Arg Asn Tyr Leu Pro Ser
His Glu Trp Ala Arg Lys Gly 165 170 175 Gly Trp Asn Ile Ala Asp Cys
Val Ser His Ala Tyr Asp Leu Glu Ala 180 185 190 Met His Val Gly Thr
Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 195 200 205 Arg Arg Leu
Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 210 215 220 Arg
Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 225 230
235 240 Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn
Cys 245 250 255 Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu
Thr Leu Lys 260 265 270 Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr
Ala Arg Gly Lys Leu 275 280 285 Cys Asp Arg Asp Ala Val Ala Arg Ala
Leu Glu Ser Gly Arg Leu Ala 290 295 300 Gly Tyr Ala Gly Asp Val Trp
Phe Pro Gln Pro Ala Pro Lys Asp His 305 310 315 320 Pro Trp Arg Thr
Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 325 330 335 Thr Thr
Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 340 345 350
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355
360 365 Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser
Lys 370 375 380 Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe
Lys Lys Ala 385 390 395 400 Ala Glu Asn Ser Ser Ser Val Asp Lys Leu
Ala Ala Ala Leu Glu His 405 410 415 His His His His His 420
<210> SEQ ID NO 36 <211> LENGTH: 401 <212> TYPE:
PRT <213> ORGANISM: Mycobacterium vaccae <400>
SEQUENCE: 36 Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val
Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys
Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile Leu Pro Thr Pro Lys
Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu Leu Gly Ser Val Ser
Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 Glu Ser Asn Gly His
Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80 Asp Ser Val
Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85 90 95 Gln
Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 100 105
110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val
115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu
Val Thr 130 135 140 Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val
Met Met Ile Leu 145 150 155 160 Ser Leu Val Arg Asn Tyr Leu Pro Ser
His Glu Trp Ala Arg Lys Gly 165 170 175 Gly Trp Asn Ile Ala Asp Cys
Val Ser His Ala Tyr Asp Leu Glu Ala 180 185 190 Met His Val Gly Thr
Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 195 200 205 Arg Arg Leu
Ala Pro Phe Asp Val His Leu His Tyr Thr Asp Arg His 210 215 220 Arg
Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 225 230
235 240 Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn
Cys 245 250 255 Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu
Thr Leu Lys 260 265 270 Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr
Ala Arg Gly Lys Leu 275 280 285 Cys Asp Arg Asp Ala Val Ala Arg Ala
Leu Glu Ser Gly Arg Leu Ala 290 295 300 Gly Tyr Ala Gly Asp Val Trp
Phe Pro Gln Pro Ala Pro Lys Asp His 305 310 315 320 Pro Trp Arg Thr
Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 325 330 335 Thr Thr
Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 340 345 350
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355
360 365 Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser
Lys 370 375 380 Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe
Lys Lys Ala 385 390 395 400 Val <210> SEQ ID NO 37
<211> LENGTH: 263 <212> TYPE: PRT <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION:
derived from Collinsella aerofaciens (G39D) <400> SEQUENCE:
37 Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala
1 5 10 15 Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala
Gly Gly 20 25 30 Met Asn Val Val Met Val Asp Arg Arg Glu Glu Lys
Leu Asn Val Leu 35 40 45 Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val
Glu Thr Lys Val Val Arg 50 55 60 Ala Asp Phe Ser Gln Pro Gly Ala
Ala Glu Thr Val Phe Ala Ala Thr 65 70 75 80 Glu Gly Leu Asp Met Gly
Phe Met Ser Tyr Val Ala Cys Leu His Ser 85 90 95 Phe Gly Lys Ile
Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 100 105 110 Asn Val
Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 115 120 125
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 130
135 140 Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly
Lys 145 150 155 160 Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys
Glu Cys Glu Gly 165 170 175 Thr Gly Val Asp Val Glu Val Ile Thr Leu
Gly Thr Thr Leu Thr Pro 180 185 190 Ser Leu Leu Ser Asn Leu Pro Gly
Gly Pro Gln Gly Glu Ala Val Met 195 200 205 Lys Ile Ala Leu Thr Pro
Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210 215 220 Leu Gly Lys Glu
Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 225 230 235 240 Val
His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 245 250
255 Met Gly Ser Phe Tyr Arg Asp 260 <210> SEQ ID NO 38
<211> LENGTH: 263 <212> TYPE: PRT <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION:
derived from Collinsella aerofaciens (G39D R40L) <400>
SEQUENCE: 38 Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile
Leu Gly Ala 1 5 10 15 Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys
Ile Ala Ala Gly Gly 20 25 30 Met Asn Val Val Met Val Asp Leu Arg
Glu Glu Lys Leu Asn Val Leu 35 40 45 Ala Gly Glu Ile Arg Glu Thr
Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60 Ala Asp Phe Ser Gln
Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65 70 75 80 Glu Gly Leu
Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 85 90 95 Phe
Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 100 105
110 Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg
115 120 125 Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser
Ser Met 130 135 140 Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr
Gly Ala Gly Lys 145 150 155 160 Ala Phe Ile Leu Lys Met Thr Glu Ala
Val Ala Cys Glu Cys Glu Gly 165 170 175 Thr Gly Val Asp Val Glu Val
Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185 190 Ser Leu Leu Ser Asn
Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 195 200 205 Lys Ile Ala
Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210 215 220 Leu
Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 225 230
235 240 Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg
Tyr 245 250 255 Met Gly Ser Phe Tyr Arg Asp 260 <210> SEQ ID
NO 39 <211> LENGTH: 263 <212> TYPE: PRT <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: derived from Collinsella aerofaciens (G39D R40I)
<400> SEQUENCE: 39 Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp
Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu Gly Val Gly Lys Ala Phe
Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30 Met Asn Val Val Met Val
Asp Ile Arg Glu Glu Lys Leu Asn Val Leu 35 40 45 Ala Gly Glu Ile
Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60 Ala Asp
Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65 70 75 80
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 85
90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met
Ile 100 105 110 Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His
Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile
Asn Val Ser Ser Met 130 135 140 Thr Gly Ile Ser Ser Ser Pro Trp Asn
Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160 Ala Phe Ile Leu Lys Met
Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175 Thr Gly Val Asp
Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185 190 Ser Leu
Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 195 200 205
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210
215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp
Ser 225 230 235 240 Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu
Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe Tyr Arg Asp 260
<210> SEQ ID NO 40 <211> LENGTH: 263 <212> TYPE:
PRT <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: derived from Collinsella aerofaciens
<400> SEQUENCE: 40 Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp
Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu Gly Val Gly Lys Ala Phe
Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30 Met Asn Val Val Met Val
Asp Val Arg Glu Glu Lys Leu Asn Val Leu 35 40 45 Ala Gly Glu Ile
Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60 Ala Asp
Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65 70 75 80
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 85
90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met
Ile 100 105 110 Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His
Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile
Asn Val Ser Ser Met 130 135 140 Thr Gly Ile Ser Ser Ser Pro Trp Asn
Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160 Ala Phe Ile Leu Lys Met
Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175 Thr Gly Val Asp
Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185 190 Ser Leu
Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 195 200 205
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210
215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp
Ser 225 230 235 240 Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu
Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe Tyr Arg Asp 260
<210> SEQ ID NO 41 <211> LENGTH: 26 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 41
cgtcgtcatg gtcgaccgtc gcgagg 26 <210> SEQ ID NO 42
<211> LENGTH: 26 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 42 cctcgcgacg gtcgaccatg acgacg 26
<210> SEQ ID NO 43 <211> LENGTH: 26 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 43
cgtcgtcatg gtcgacctgc gcgagg 26 <210> SEQ ID NO 44
<211> LENGTH: 31 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 44 ccgccgcatc cataccgcca gttgtttacc c
31 <210> SEQ ID NO 45 <211> LENGTH: 26 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 45
cgtcgtcatg gtcgacattc gcgagg 26 <210> SEQ ID NO 46
<211> LENGTH: 26 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 46 cgtcgtcatg gtcgacgttc gcgagg 26
<210> SEQ ID NO 47 <211> LENGTH: 786 <212> TYPE:
DNA <213> ORGANISM: Bacillus subtilis <400> SEQUENCE:
47 atgtatccgg atttaaaagg aaaagtcgtc gctattacag gagctgcttc
agggctcgga 60 aaggcgatgg ccattcgctt cggcaaggag caggcaaaag
tggttatcaa ctattatagt 120 aataaacaag atccgaacga ggtaaaagaa
gaggtcatca aggcgggcgg tgaagctgtt 180 gtcgtccaag gagatgtcac
gaaagaggaa gatgtaaaaa atatcgtgca aacggcaatt 240 aaggagttcg
gcacactcga tattatgatt aataatgccg gtcttgaaaa tcctgtgcca 300
tctcacgaaa tgccgctcaa ggattgggat aaagtcatcg gcacgaactt aacgggtgcc
360 tttttaggaa gccgtgaagc gattaaatat ttcgtagaaa acgatatcaa
gggaaatgtc 420 attaacatgt ccagtgtgca cgaagtgatt ccttggccgt
tatttgtcca ctatgcggca 480 agtaaaggcg ggataaagct gatgacagaa
acattagcgt tggaatacgc gccgaagggc 540 attcgcgtca ataatattgg
gccaggtgcg atcaacacgc caatcaatgc tgaaaaattc 600 gctgacccta
aacagaaagc tgatgtagaa agcatgattc caatgggata tatcggcgaa 660
ccggaggaga tcgccgcagt agcagcctgg cttgcttcga aggaagccag ctacgtcaca
720 ggcatcacgt tattcgcgga cggcggtatg acacaatatc cttcattcca
ggcaggccgc 780 ggttaa 786 <210> SEQ ID NO 48 <211>
LENGTH: 261 <212> TYPE: PRT <213> ORGANISM: Bacillus
subtilis <400> SEQUENCE: 48 Met Tyr Pro Asp Leu Lys Gly Lys
Val Val Ala Ile Thr Gly Ala Ala 1 5 10 15 Ser Gly Leu Gly Lys Ala
Met Ala Ile Arg Phe Gly Lys Glu Gln Ala 20 25 30 Lys Val Val Ile
Asn Tyr Tyr Ser Asn Lys Gln Asp Pro Asn Glu Val 35 40 45 Lys Glu
Glu Val Ile Lys Ala Gly Gly Glu Ala Val Val Val Gln Gly 50 55 60
Asp Val Thr Lys Glu Glu Asp Val Lys Asn Ile Val Gln Thr Ala Ile 65
70 75 80 Lys Glu Phe Gly Thr Leu Asp Ile Met Ile Asn Asn Ala Gly
Leu Glu 85 90 95 Asn Pro Val Pro Ser His Glu Met Pro Leu Lys Asp
Trp Asp Lys Val 100 105 110 Ile Gly Thr Asn Leu Thr Gly Ala Phe Leu
Gly Ser Arg Glu Ala Ile 115 120 125 Lys Tyr Phe Val Glu Asn Asp Ile
Lys Gly Asn Val Ile Asn Met Ser 130 135 140 Ser Val His Glu Val Ile
Pro Trp Pro Leu Phe Val His Tyr Ala Ala 145 150 155 160 Ser Lys Gly
Gly Ile Lys Leu Met Thr Glu Thr Leu Ala Leu Glu Tyr 165 170 175 Ala
Pro Lys Gly Ile Arg Val Asn Asn Ile Gly Pro Gly Ala Ile Asn 180 185
190 Thr Pro Ile Asn Ala Glu Lys Phe Ala Asp Pro Lys Gln Lys Ala Asp
195 200 205 Val Glu Ser Met Ile Pro Met Gly Tyr Ile Gly Glu Pro Glu
Glu Ile 210 215 220 Ala Ala Val Ala Ala Trp Leu Ala Ser Lys Glu Ala
Ser Tyr Val Thr 225 230 235 240 Gly Ile Thr Leu Phe Ala Asp Gly Gly
Met Thr Gln Tyr Pro Ser Phe 245 250 255 Gln Ala Gly Arg Gly 260
<210> SEQ ID NO 49 <211> LENGTH: 7404 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: Vector pFr7(D) <400> SEQUENCE:
49 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gcaagcttct 180 agtcgcggta gaacgacccc
atgtagcgga tgtactcgtc ctcggtgtgg tttgccttcc 240 agtcgtggac
ggagtccttg ttgcgctggc cggcgatgac ggagagctcc ttacccagct 300
tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc gatcttcatg acggcctcgc
360 cctgcgggcc gccggggagg ttggacagca ggctgggggt tagggtggtg
ccgagggtga 420 tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc
cacggcctcg gtcatcttga 480 ggatgaaggc cttgcccgcg ccgtactggc
cgttccaggg gctggagctg atgccggtca 540 tcgacgagac gttgatcacg
gcgccgcggt cctgggcggc aaagatccgc atgtagtggt 600 ggaagcactt
gaggaaggtc acgacgttga cgttgatcat ggcctcgtgc ttctcccagg 660
gggtgtcctg gatcttaccg aagctgtgca ggcaggccac gtagctcatg aagcccatgt
720 ccaggccctc ggtcgcggcg aagacggtct cggcagcgcc gggctggcta
aagtcggcgc 780 gcacgacctt ggtctccacg ccgtaggtct cgcggatctc
gcctgcgagc acgttcagct 840 tctcctcgcg acggtcgacc atgacgacgt
tcatgccgcc ggcggcgatc ttctcgcaga 900 acgccttgcc gacgccctcg
gtcgcgccca ggatcaggcc ccactcaccg tacttctccc 960 tcaggttcat
gtatatctcc tttcacgaat tctcagccgc cttcttgaac ttggcggcct 1020
cttccgaacc gccggtggca ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag
1080 cgccgccctg cacgatgagg tattcgtcgc ggatcggacg gccctcgaag
aagcactcca 1140 ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt
cagcgtggtg ccggagatgt 1200 gcggggtcat gccgttatag ggcatcgtcc
gccaggggtg gtccttcggc gccggctgcg 1260 ggaaccacac gtcgccggca
tagccggcca gccggccgga ttcgagcgca cgtgccacgg 1320 catcgcggtc
gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca cgcttgaaca 1380
gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg gtgcagcggg cagttcagcg
1440 tcaccacgtc gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg
aggttgagct 1500 ccttctcgac cgattccggc aggcggtgac ggccggtgta
gtgcaggtgc acgtcgaacg 1560 gcgccagacg gcgcagcacc gcgagaccga
tgcggccggc ggccacggtg ccgacatgca 1620 tcgcctcgag gtcgtaggcg
tgggagacgc agtcggcgat gttccagccg cccttccgcg 1680 cccattcgtg
cgagggcaga tagttgcgca ccagcgacag gatcatcatc accacatgct 1740
cggcgacgct gatcgagttg cagtaggtga cttccgccac ggtgacgttg cggtcgatag
1800 ccgactgaag atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc
ttcaggttct 1860 tggccttggc gatgcgctcg ggcgtcagat aggccggcca
gaagggctgg gagatgacga 1920 catccgcatc gaccagctcg cgctcgaaca
ccgagtcggg gccgtccttg tcggaggtca 1980 cgaccagggt gtggccgttg
gattcgagat attcgcgcag gccgagctcg ccggagacgg 2040 agccgagcaa
ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag atctggccgc 2100
ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga
2160 ccggatcatc gtaaagaacg cacaggacct ttgccatcat atgtatatct
ccttcttaaa 2220 gttaaacaaa attatttcta gaggggaatt gttatccgct
cacaattccc ctatagtgag 2280 tcgtattaat ttcgcgggat cgagatctcg
atcctctacg ccggacgcat cgtggccggc 2340 atcaccggcg ccacaggtgc
ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2400 gatcgggctc
gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 2460
cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
2520 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
gcataaggga 2580 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
tttcgcggta tggcatgata 2640 gcgcccggaa gagagtcaat tcagggtggt
gaatgtgaaa ccagtaacgt tatacgatgt 2700 cgcagagtat gccggtgtct
cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2760 cgtttctgcg
aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 2820
caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
2880 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
gcgccgatca 2940 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
ggcgtcgaag cctgtaaagc 3000 ggcggtgcac aatcttctcg cgcaacgcgt
cagtgggctg atcattaact atccgctgga 3060 tgaccaggat gccattgctg
tggaagctgc ctgcactaat gttccggcgt tatttcttga 3120 tgtctctgac
cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3180
gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
3240 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
ctcgcaatca 3300 aattcagccg atagcggaac gggaaggcga ctggagtgcc
atgtccggtt ttcaacaaac 3360 catgcaaatg ctgaatgagg gcatcgttcc
cactgcgatg ctggttgcca acgatcagat 3420 ggcgctgggc gcaatgcgcg
ccattaccga gtccgggctg cgcgttggtg cggatatctc 3480 ggtagtggga
tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3540
caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
3600 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
gaaaaaccac 3660 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
gccgattcat taatgcagct 3720 ggcacgacag gtttcccgac tggaaagcgg
gcagtgagcg caacgcaatt aatgtaagtt 3780 agctcactca ttaggcaccg
ggatctcgac cgatgccctt gagagccttc aacccagtca 3840 gctccttccg
gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3900
tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
3960 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
ttgcacgccc 4020 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
cggcgagaag caggccatta 4080 tcgccggcat ggcggcccca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc 4140 taggctggcg gggttgcctt
actggttagc agaatgaatc accgatacgc gagcgaacgt 4200 gaagcgactg
ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 4260
tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
4320 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc 4380 gctggcattg accctgagtg atttttctct ggtcccgccg
catccatacc gccagttgtt 4440 taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca 4500 tcctctctcg tttcatcggt
atcattaccc ccatgaacag aaatccccct tacacggagg 4560 catcagtgac
caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 4620
cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
4680 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
ttcggtgatg 4740 acggtgaaaa cctctgacac atgcagctcc cggagacggt
cacagcttgt ctgtaagcgg 4800 atgccgggag cagacaagcc cgtcagggcg
cgtcagcggg tgttggcggg tgtcggggcg 4860 cagccatgac ccagtcacgt
agcgatagcg gagtgtatac tggcttaact atgcggcatc 4920 agagcagatt
gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 4980
aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5040 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca 5100 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
gccagcaaaa ggccaggaac 5160 cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac 5220 aaaaatcgac gctcaagtca
gaggtggcga aacccgacag gactataaag ataccaggcg 5280 tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5340
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5400 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag 5460 cccgaccgct gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac 5520 ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt 5580 gctacagagt tcttgaagtg
gtggcctaac tacggctaca ctagaaggac agtatttggt 5640 atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5700
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5760 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac 5820 gaaaactcac gttaagggat tttggtcatg agattatcaa
aaaggatctt cacctagatc 5880 cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct 5940 gacagttacc aatgcttaat
cagtgaggca cctatctcag cgatctgtct atttcgttca 6000 tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6060
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6120 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc 6180 atccagtcta ttaattgttg ccgggaagct agagtaagta
gttcgccagt taatagtttg 6240 cgcaacgttg ttgccattgc tgcaggcatc
gtggtgtcac gctcgtcgtt tggtatggct 6300 tcattcagct ccggttccca
acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6360 aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6420
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
6480 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg 6540 agttgctctt gcccggcgtc aatacgggat aataccgcgc
cacatagcag aactttaaaa 6600 gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg 6660 agatccagtt cgatgtaacc
cactcgtgca cccaactgat cttcagcatc ttttactttc 6720 accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6780
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6840 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata 6900 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
ttgtaaacgt taatattttg 6960 ttaaaattcg cgttaaattt ttgttaaatc
agctcatttt ttaaccaata ggccgaaatc 7020 ggcaaaatcc cttataaatc
aaaagaatag accgagatag ggttgagtgt tgttccagtt 7080 tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7140
tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7200 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
ttgacgggga 7260 aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg 7320 ctggcaagtg tagcggtcac gctgcgcgta
accaccacac ccgccgcgct taatgcgccg 7380 ctacagggcg cgtcccattc gcca
7404 <210> SEQ ID NO 50 <211> LENGTH: 7404 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: Vector pFr7(DL) <400>
SEQUENCE: 50 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gcaagcttct 180 agtcgcggta gaacgacccc
atgtagcgga tgtactcgtc ctcggtgtgg tttgccttcc 240 agtcgtggac
ggagtccttg ttgcgctggc cggcgatgac ggagagctcc ttacccagct 300
tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc gatcttcatg acggcctcgc
360 cctgcgggcc gccggggagg ttggacagca ggctgggggt tagggtggtg
ccgagggtga 420 tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc
cacggcctcg gtcatcttga 480 ggatgaaggc cttgcccgcg ccgtactggc
cgttccaggg gctggagctg atgccggtca 540 tcgacgagac gttgatcacg
gcgccgcggt cctgggcggc aaagatccgc atgtagtggt 600 ggaagcactt
gaggaaggtc acgacgttga cgttgatcat ggcctcgtgc ttctcccagg 660
gggtgtcctg gatcttaccg aagctgtgca ggcaggccac gtagctcatg aagcccatgt
720 ccaggccctc ggtcgcggcg aagacggtct cggcagcgcc gggctggcta
aagtcggcgc 780 gcacgacctt ggtctccacg ccgtaggtct cgcggatctc
gcctgcgagc acgttcagct 840 tctcctcgcg caggtcgacc atgacgacgt
tcatgccgcc ggcggcgatc ttctcgcaga 900 acgccttgcc gacgccctcg
gtcgcgccca ggatcaggcc ccactcaccg tacttctccc 960 tcaggttcat
gtatatctcc tttcacgaat tctcagccgc cttcttgaac ttggcggcct 1020
cttccgaacc gccggtggca ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag
1080 cgccgccctg cacgatgagg tattcgtcgc ggatcggacg gccctcgaag
aagcactcca 1140 ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt
cagcgtggtg ccggagatgt 1200 gcggggtcat gccgttatag ggcatcgtcc
gccaggggtg gtccttcggc gccggctgcg 1260 ggaaccacac gtcgccggca
tagccggcca gccggccgga ttcgagcgca cgtgccacgg 1320 catcgcggtc
gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca cgcttgaaca 1380
gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg gtgcagcggg cagttcagcg
1440 tcaccacgtc gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg
aggttgagct 1500 ccttctcgac cgattccggc aggcggtgac ggccggtgta
gtgcaggtgc acgtcgaacg 1560 gcgccagacg gcgcagcacc gcgagaccga
tgcggccggc ggccacggtg ccgacatgca 1620 tcgcctcgag gtcgtaggcg
tgggagacgc agtcggcgat gttccagccg cccttccgcg 1680 cccattcgtg
cgagggcaga tagttgcgca ccagcgacag gatcatcatc accacatgct 1740
cggcgacgct gatcgagttg cagtaggtga cttccgccac ggtgacgttg cggtcgatag
1800 ccgactgaag atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc
ttcaggttct 1860 tggccttggc gatgcgctcg ggcgtcagat aggccggcca
gaagggctgg gagatgacga 1920 catccgcatc gaccagctcg cgctcgaaca
ccgagtcggg gccgtccttg tcggaggtca 1980 cgaccagggt gtggccgttg
gattcgagat attcgcgcag gccgagctcg ccggagacgg 2040 agccgagcaa
ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag atctggccgc 2100
ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga
2160 ccggatcatc gtaaagaacg cacaggacct ttgccatcat atgtatatct
ccttcttaaa 2220 gttaaacaaa attatttcta gaggggaatt gttatccgct
cacaattccc ctatagtgag 2280 tcgtattaat ttcgcgggat cgagatctcg
atcctctacg ccggacgcat cgtggccggc 2340 atcaccggcg ccacaggtgc
ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2400 gatcgggctc
gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 2460
cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
2520 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
gcataaggga 2580 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
tttcgcggta tggcatgata 2640 gcgcccggaa gagagtcaat tcagggtggt
gaatgtgaaa ccagtaacgt tatacgatgt 2700 cgcagagtat gccggtgtct
cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2760 cgtttctgcg
aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 2820
caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
2880 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
gcgccgatca 2940 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
ggcgtcgaag cctgtaaagc 3000 ggcggtgcac aatcttctcg cgcaacgcgt
cagtgggctg atcattaact atccgctgga 3060 tgaccaggat gccattgctg
tggaagctgc ctgcactaat gttccggcgt tatttcttga 3120 tgtctctgac
cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3180
gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
3240 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
ctcgcaatca 3300 aattcagccg atagcggaac gggaaggcga ctggagtgcc
atgtccggtt ttcaacaaac 3360 catgcaaatg ctgaatgagg gcatcgttcc
cactgcgatg ctggttgcca acgatcagat 3420 ggcgctgggc gcaatgcgcg
ccattaccga gtccgggctg cgcgttggtg cggatatctc 3480 ggtagtggga
tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3540
caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
3600 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
gaaaaaccac 3660 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
gccgattcat taatgcagct 3720 ggcacgacag gtttcccgac tggaaagcgg
gcagtgagcg caacgcaatt aatgtaagtt 3780 agctcactca ttaggcaccg
ggatctcgac cgatgccctt gagagccttc aacccagtca 3840 gctccttccg
gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3900
tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
3960 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
ttgcacgccc 4020 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
cggcgagaag caggccatta 4080 tcgccggcat ggcggcccca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc 4140 taggctggcg gggttgcctt
actggttagc agaatgaatc accgatacgc gagcgaacgt 4200 gaagcgactg
ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 4260
tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
4320 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc 4380 gctggcattg accctgagtg atttttctct ggtcccgccg
catccatacc gccagttgtt 4440 taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca 4500 tcctctctcg tttcatcggt
atcattaccc ccatgaacag aaatccccct tacacggagg 4560 catcagtgac
caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 4620
cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
4680 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
ttcggtgatg 4740 acggtgaaaa cctctgacac atgcagctcc cggagacggt
cacagcttgt ctgtaagcgg 4800 atgccgggag cagacaagcc cgtcagggcg
cgtcagcggg tgttggcggg tgtcggggcg 4860 cagccatgac ccagtcacgt
agcgatagcg gagtgtatac tggcttaact atgcggcatc 4920 agagcagatt
gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 4980
aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5040 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca 5100 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
gccagcaaaa ggccaggaac 5160 cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac 5220 aaaaatcgac gctcaagtca
gaggtggcga aacccgacag gactataaag ataccaggcg 5280 tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5340
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5400 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag 5460 cccgaccgct gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac 5520 ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt 5580 gctacagagt tcttgaagtg
gtggcctaac tacggctaca ctagaaggac agtatttggt 5640 atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5700
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5760 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac 5820 gaaaactcac gttaagggat tttggtcatg agattatcaa
aaaggatctt cacctagatc 5880 cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct 5940 gacagttacc aatgcttaat
cagtgaggca cctatctcag cgatctgtct atttcgttca 6000 tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6060
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6120 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc 6180 atccagtcta ttaattgttg ccgggaagct agagtaagta
gttcgccagt taatagtttg 6240 cgcaacgttg ttgccattgc tgcaggcatc
gtggtgtcac gctcgtcgtt tggtatggct 6300 tcattcagct ccggttccca
acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6360 aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6420
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
6480 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg 6540 agttgctctt gcccggcgtc aatacgggat aataccgcgc
cacatagcag aactttaaaa 6600 gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg 6660 agatccagtt cgatgtaacc
cactcgtgca cccaactgat cttcagcatc ttttactttc 6720 accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6780
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6840 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata 6900 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
ttgtaaacgt taatattttg 6960 ttaaaattcg cgttaaattt ttgttaaatc
agctcatttt ttaaccaata ggccgaaatc 7020 ggcaaaatcc cttataaatc
aaaagaatag accgagatag ggttgagtgt tgttccagtt 7080 tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7140
tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7200 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
ttgacgggga 7260 aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg 7320 ctggcaagtg tagcggtcac gctgcgcgta
accaccacac ccgccgcgct taatgcgccg 7380 ctacagggcg cgtcccattc gcca
7404 <210> SEQ ID NO 51 <211> LENGTH: 7404 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: Vector pFr7(DI) <400>
SEQUENCE: 51 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gcaagcttct 180 agtcgcggta gaacgacccc
atgtagcgga tgtactcgtc ctcggtgtgg tttgccttcc 240 agtcgtggac
ggagtccttg ttgcgctggc cggcgatgac ggagagctcc ttacccagct 300
tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc gatcttcatg acggcctcgc
360 cctgcgggcc gccggggagg ttggacagca ggctgggggt tagggtggtg
ccgagggtga 420 tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc
cacggcctcg gtcatcttga 480 ggatgaaggc cttgcccgcg ccgtactggc
cgttccaggg gctggagctg atgccggtca 540 tcgacgagac gttgatcacg
gcgccgcggt cctgggcggc aaagatccgc atgtagtggt 600 ggaagcactt
gaggaaggtc acgacgttga cgttgatcat ggcctcgtgc ttctcccagg 660
gggtgtcctg gatcttaccg aagctgtgca ggcaggccac gtagctcatg aagcccatgt
720 ccaggccctc ggtcgcggcg aagacggtct cggcagcgcc gggctggcta
aagtcggcgc 780 gcacgacctt ggtctccacg ccgtaggtct cgcggatctc
gcctgcgagc acgttcagct 840 tctcctcgcg aatgtcgacc atgacgacgt
tcatgccgcc ggcggcgatc ttctcgcaga 900 acgccttgcc gacgccctcg
gtcgcgccca ggatcaggcc ccactcaccg tacttctccc 960 tcaggttcat
gtatatctcc tttcacgaat tctcagccgc cttcttgaac ttggcggcct 1020
cttccgaacc gccggtggca ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag
1080 cgccgccctg cacgatgagg tattcgtcgc ggatcggacg gccctcgaag
aagcactcca 1140 ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt
cagcgtggtg ccggagatgt 1200 gcggggtcat gccgttatag ggcatcgtcc
gccaggggtg gtccttcggc gccggctgcg 1260 ggaaccacac gtcgccggca
tagccggcca gccggccgga ttcgagcgca cgtgccacgg 1320 catcgcggtc
gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca cgcttgaaca 1380
gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg gtgcagcggg cagttcagcg
1440 tcaccacgtc gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg
aggttgagct 1500 ccttctcgac cgattccggc aggcggtgac ggccggtgta
gtgcaggtgc acgtcgaacg 1560 gcgccagacg gcgcagcacc gcgagaccga
tgcggccggc ggccacggtg ccgacatgca 1620 tcgcctcgag gtcgtaggcg
tgggagacgc agtcggcgat gttccagccg cccttccgcg 1680 cccattcgtg
cgagggcaga tagttgcgca ccagcgacag gatcatcatc accacatgct 1740
cggcgacgct gatcgagttg cagtaggtga cttccgccac ggtgacgttg cggtcgatag
1800 ccgactgaag atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc
ttcaggttct 1860 tggccttggc gatgcgctcg ggcgtcagat aggccggcca
gaagggctgg gagatgacga 1920 catccgcatc gaccagctcg cgctcgaaca
ccgagtcggg gccgtccttg tcggaggtca 1980 cgaccagggt gtggccgttg
gattcgagat attcgcgcag gccgagctcg ccggagacgg 2040 agccgagcaa
ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag atctggccgc 2100
ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga
2160 ccggatcatc gtaaagaacg cacaggacct ttgccatcat atgtatatct
ccttcttaaa 2220 gttaaacaaa attatttcta gaggggaatt gttatccgct
cacaattccc ctatagtgag 2280 tcgtattaat ttcgcgggat cgagatctcg
atcctctacg ccggacgcat cgtggccggc 2340 atcaccggcg ccacaggtgc
ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2400 gatcgggctc
gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 2460
cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
2520 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
gcataaggga 2580 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
tttcgcggta tggcatgata 2640 gcgcccggaa gagagtcaat tcagggtggt
gaatgtgaaa ccagtaacgt tatacgatgt 2700 cgcagagtat gccggtgtct
cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2760 cgtttctgcg
aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 2820
caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
2880 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
gcgccgatca 2940 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
ggcgtcgaag cctgtaaagc 3000 ggcggtgcac aatcttctcg cgcaacgcgt
cagtgggctg atcattaact atccgctgga 3060 tgaccaggat gccattgctg
tggaagctgc ctgcactaat gttccggcgt tatttcttga 3120 tgtctctgac
cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3180
gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
3240 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
ctcgcaatca 3300 aattcagccg atagcggaac gggaaggcga ctggagtgcc
atgtccggtt ttcaacaaac 3360 catgcaaatg ctgaatgagg gcatcgttcc
cactgcgatg ctggttgcca acgatcagat 3420 ggcgctgggc gcaatgcgcg
ccattaccga gtccgggctg cgcgttggtg cggatatctc 3480 ggtagtggga
tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3540
caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
3600 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
gaaaaaccac 3660 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
gccgattcat taatgcagct 3720 ggcacgacag gtttcccgac tggaaagcgg
gcagtgagcg caacgcaatt aatgtaagtt 3780 agctcactca ttaggcaccg
ggatctcgac cgatgccctt gagagccttc aacccagtca 3840 gctccttccg
gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3900
tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
3960 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
ttgcacgccc 4020 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
cggcgagaag caggccatta 4080 tcgccggcat ggcggcccca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc 4140 taggctggcg gggttgcctt
actggttagc agaatgaatc accgatacgc gagcgaacgt 4200 gaagcgactg
ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 4260
tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
4320 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc 4380 gctggcattg accctgagtg atttttctct ggtcccgccg
catccatacc gccagttgtt 4440 taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca 4500 tcctctctcg tttcatcggt
atcattaccc ccatgaacag aaatccccct tacacggagg 4560 catcagtgac
caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 4620
cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
4680 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
ttcggtgatg 4740 acggtgaaaa cctctgacac atgcagctcc cggagacggt
cacagcttgt ctgtaagcgg 4800 atgccgggag cagacaagcc cgtcagggcg
cgtcagcggg tgttggcggg tgtcggggcg 4860 cagccatgac ccagtcacgt
agcgatagcg gagtgtatac tggcttaact atgcggcatc 4920 agagcagatt
gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 4980
aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5040 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca 5100 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
gccagcaaaa ggccaggaac 5160 cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac 5220 aaaaatcgac gctcaagtca
gaggtggcga aacccgacag gactataaag ataccaggcg 5280 tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5340
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5400 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag 5460 cccgaccgct gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac 5520 ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt 5580 gctacagagt tcttgaagtg
gtggcctaac tacggctaca ctagaaggac agtatttggt 5640 atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5700
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5760 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac 5820 gaaaactcac gttaagggat tttggtcatg agattatcaa
aaaggatctt cacctagatc 5880 cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct 5940 gacagttacc aatgcttaat
cagtgaggca cctatctcag cgatctgtct atttcgttca 6000 tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6060
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6120 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc 6180 atccagtcta ttaattgttg ccgggaagct agagtaagta
gttcgccagt taatagtttg 6240 cgcaacgttg ttgccattgc tgcaggcatc
gtggtgtcac gctcgtcgtt tggtatggct 6300 tcattcagct ccggttccca
acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6360 aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6420
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
6480 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg 6540 agttgctctt gcccggcgtc aatacgggat aataccgcgc
cacatagcag aactttaaaa 6600 gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg 6660 agatccagtt cgatgtaacc
cactcgtgca cccaactgat cttcagcatc ttttactttc 6720 accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6780
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6840 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata 6900 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
ttgtaaacgt taatattttg 6960 ttaaaattcg cgttaaattt ttgttaaatc
agctcatttt ttaaccaata ggccgaaatc 7020 ggcaaaatcc cttataaatc
aaaagaatag accgagatag ggttgagtgt tgttccagtt 7080 tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7140
tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7200 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
ttgacgggga 7260 aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg 7320 ctggcaagtg tagcggtcac gctgcgcgta
accaccacac ccgccgcgct taatgcgccg 7380 ctacagggcg cgtcccattc gcca
7404 <210> SEQ ID NO 52 <211> LENGTH: 7404 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: Vector pFr7(DV) <400>
SEQUENCE: 52 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gcaagcttct 180 agtcgcggta gaacgacccc
atgtagcgga tgtactcgtc ctcggtgtgg tttgccttcc 240 agtcgtggac
ggagtccttg ttgcgctggc cggcgatgac ggagagctcc ttacccagct 300
tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc gatcttcatg acggcctcgc
360 cctgcgggcc gccggggagg ttggacagca ggctgggggt tagggtggtg
ccgagggtga 420 tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc
cacggcctcg gtcatcttga 480 ggatgaaggc cttgcccgcg ccgtactggc
cgttccaggg gctggagctg atgccggtca 540 tcgacgagac gttgatcacg
gcgccgcggt cctgggcggc aaagatccgc atgtagtggt 600 ggaagcactt
gaggaaggtc acgacgttga cgttgatcat ggcctcgtgc ttctcccagg 660
gggtgtcctg gatcttaccg aagctgtgca ggcaggccac gtagctcatg aagcccatgt
720 ccaggccctc ggtcgcggcg aagacggtct cggcagcgcc gggctggcta
aagtcggcgc 780 gcacgacctt ggtctccacg ccgtaggtct cgcggatctc
gcctgcgagc acgttcagct 840 tctcctcgcg aacgtcgacc atgacgacgt
tcatgccgcc ggcggcgatc ttctcgcaga 900 acgccttgcc gacgccctcg
gtcgcgccca ggatcaggcc ccactcaccg tacttctccc 960 tcaggttcat
gtatatctcc tttcacgaat tctcagccgc cttcttgaac ttggcggcct 1020
cttccgaacc gccggtggca ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag
1080 cgccgccctg cacgatgagg tattcgtcgc ggatcggacg gccctcgaag
aagcactcca 1140 ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt
cagcgtggtg ccggagatgt 1200 gcggggtcat gccgttatag ggcatcgtcc
gccaggggtg gtccttcggc gccggctgcg 1260 ggaaccacac gtcgccggca
tagccggcca gccggccgga ttcgagcgca cgtgccacgg 1320 catcgcggtc
gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca cgcttgaaca 1380
gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg gtgcagcggg cagttcagcg
1440 tcaccacgtc gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg
aggttgagct 1500 ccttctcgac cgattccggc aggcggtgac ggccggtgta
gtgcaggtgc acgtcgaacg 1560 gcgccagacg gcgcagcacc gcgagaccga
tgcggccggc ggccacggtg ccgacatgca 1620 tcgcctcgag gtcgtaggcg
tgggagacgc agtcggcgat gttccagccg cccttccgcg 1680 cccattcgtg
cgagggcaga tagttgcgca ccagcgacag gatcatcatc accacatgct 1740
cggcgacgct gatcgagttg cagtaggtga cttccgccac ggtgacgttg cggtcgatag
1800 ccgactgaag atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc
ttcaggttct 1860 tggccttggc gatgcgctcg ggcgtcagat aggccggcca
gaagggctgg gagatgacga 1920 catccgcatc gaccagctcg cgctcgaaca
ccgagtcggg gccgtccttg tcggaggtca 1980 cgaccagggt gtggccgttg
gattcgagat attcgcgcag gccgagctcg ccggagacgg 2040 agccgagcaa
ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag atctggccgc 2100
ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga
2160 ccggatcatc gtaaagaacg cacaggacct ttgccatcat atgtatatct
ccttcttaaa 2220 gttaaacaaa attatttcta gaggggaatt gttatccgct
cacaattccc ctatagtgag 2280 tcgtattaat ttcgcgggat cgagatctcg
atcctctacg ccggacgcat cgtggccggc 2340 atcaccggcg ccacaggtgc
ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2400 gatcgggctc
gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 2460
cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
2520 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
gcataaggga 2580 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
tttcgcggta tggcatgata 2640 gcgcccggaa gagagtcaat tcagggtggt
gaatgtgaaa ccagtaacgt tatacgatgt 2700 cgcagagtat gccggtgtct
cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2760 cgtttctgcg
aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 2820
caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
2880 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
gcgccgatca 2940 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
ggcgtcgaag cctgtaaagc 3000 ggcggtgcac aatcttctcg cgcaacgcgt
cagtgggctg atcattaact atccgctgga 3060 tgaccaggat gccattgctg
tggaagctgc ctgcactaat gttccggcgt tatttcttga 3120 tgtctctgac
cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3180
gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
3240 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
ctcgcaatca 3300 aattcagccg atagcggaac gggaaggcga ctggagtgcc
atgtccggtt ttcaacaaac 3360 catgcaaatg ctgaatgagg gcatcgttcc
cactgcgatg ctggttgcca acgatcagat 3420 ggcgctgggc gcaatgcgcg
ccattaccga gtccgggctg cgcgttggtg cggatatctc 3480 ggtagtggga
tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3540
caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
3600 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
gaaaaaccac 3660 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
gccgattcat taatgcagct 3720 ggcacgacag gtttcccgac tggaaagcgg
gcagtgagcg caacgcaatt aatgtaagtt 3780 agctcactca ttaggcaccg
ggatctcgac cgatgccctt gagagccttc aacccagtca 3840 gctccttccg
gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3900
tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
3960 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
ttgcacgccc 4020 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
cggcgagaag caggccatta 4080 tcgccggcat ggcggcccca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc 4140 taggctggcg gggttgcctt
actggttagc agaatgaatc accgatacgc gagcgaacgt 4200 gaagcgactg
ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 4260
tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
4320 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc 4380 gctggcattg accctgagtg atttttctct ggtcccgccg
catccatacc gccagttgtt 4440 taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca 4500 tcctctctcg tttcatcggt
atcattaccc ccatgaacag aaatccccct tacacggagg 4560 catcagtgac
caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 4620
cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
4680 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
ttcggtgatg 4740 acggtgaaaa cctctgacac atgcagctcc cggagacggt
cacagcttgt ctgtaagcgg 4800 atgccgggag cagacaagcc cgtcagggcg
cgtcagcggg tgttggcggg tgtcggggcg 4860 cagccatgac ccagtcacgt
agcgatagcg gagtgtatac tggcttaact atgcggcatc 4920 agagcagatt
gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 4980
aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5040 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca 5100 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
gccagcaaaa ggccaggaac 5160 cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac 5220 aaaaatcgac gctcaagtca
gaggtggcga aacccgacag gactataaag ataccaggcg 5280 tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5340
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5400 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag 5460 cccgaccgct gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac 5520 ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt 5580 gctacagagt tcttgaagtg
gtggcctaac tacggctaca ctagaaggac agtatttggt 5640 atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5700
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5760 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac 5820 gaaaactcac gttaagggat tttggtcatg agattatcaa
aaaggatctt cacctagatc 5880 cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct 5940 gacagttacc aatgcttaat
cagtgaggca cctatctcag cgatctgtct atttcgttca 6000 tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6060
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6120 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc 6180 atccagtcta ttaattgttg ccgggaagct agagtaagta
gttcgccagt taatagtttg 6240 cgcaacgttg ttgccattgc tgcaggcatc
gtggtgtcac gctcgtcgtt tggtatggct 6300 tcattcagct ccggttccca
acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6360 aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6420
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
6480 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg 6540 agttgctctt gcccggcgtc aatacgggat aataccgcgc
cacatagcag aactttaaaa 6600 gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg 6660 agatccagtt cgatgtaacc
cactcgtgca cccaactgat cttcagcatc ttttactttc 6720 accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6780
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6840 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata 6900 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
ttgtaaacgt taatattttg 6960 ttaaaattcg cgttaaattt ttgttaaatc
agctcatttt ttaaccaata ggccgaaatc 7020 ggcaaaatcc cttataaatc
aaaagaatag accgagatag ggttgagtgt tgttccagtt 7080 tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7140
tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7200 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
ttgacgggga 7260 aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg 7320 ctggcaagtg tagcggtcac gctgcgcgta
accaccacac ccgccgcgct taatgcgccg 7380 ctacagggcg cgtcccattc gcca
7404 <210> SEQ ID NO 53 <211> LENGTH: 27 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer "G39D for" <400>
SEQUENCE: 53 gtcgtcatgg tcgaccgtcg cgaggag 27 <210> SEQ ID NO
54 <211> LENGTH: 27 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer "G39D_rev" <400> SEQUENCE: 54
ctcctcgcga cggtcgacca tgacgac 27 <210> SEQ ID NO 55
<211> LENGTH: 24 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer "G39D/R40I_for" <400> SEQUENCE: 55 gtcatggtcg
acattcgcga ggag 24 <210> SEQ ID NO 56 <211> LENGTH: 24
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: PCR Primer "G39D/R40I_rev"
<400> SEQUENCE: 56 ctcctcgcga atgtcgacca tgac 24 <210>
SEQ ID NO 57 <211> LENGTH: 27 <212> TYPE: DNA
<213> ORGANISM: artificial <220> FEATURE: <223>
OTHER INFORMATION: PCR Primer "R40D_for" <400> SEQUENCE: 57
gtcatggtcg gcgatcgcga ggagaag 27 <210> SEQ ID NO 58
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer "R40D_rev" <400> SEQUENCE: 58 cttctcctcg cgatcgccga
ccatgac 27 <210> SEQ ID NO 59 <211> LENGTH: 30
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: PCR Primer "R40D/R41I_for"
<400> SEQUENCE: 59 gtcatggtcg gcgatatcga ggagaagctg 30
<210> SEQ ID NO 60 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer "R40D/R41I_rev"
<400> SEQUENCE: 60 cagcttctcc tcgatatcgc cgaccatgac 30
<210> SEQ ID NO 61 <211> LENGTH: 27 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer "DIN_for" <400>
SEQUENCE: 61 atggtcgaca ttaacgagga gaagctg 27 <210> SEQ ID NO
62 <211> LENGTH: 27 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer "DIN_rev" <400> SEQUENCE: 62
cagcttctcc tcgttaatgt cgaccat 27 <210> SEQ ID NO 63
<211> LENGTH: 8321 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: Plasmid pFr3T7(D) <400> SEQUENCE: 63 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gcctagtcgc 180 ggtagaacga ccccatgtag cggatgtact cgtcctcggt
gtggtttgcc ttccagtcgt 240 ggacggagtc cttgttgcgc tggccggcga
tgacggagag ctccttaccc agcttctcaa 300 aggcctcgtc aacgcactcc
tcgggggtga gggcgatctt catgacggcc tcgccctgcg 360 ggccgccggg
gaggttggac agcaggctgg gggttagggt ggtgccgagg gtgatgacct 420
cgacgtcgac gccggtgccc tcgcactcgc aggccacggc ctcggtcatc ttgaggatga
480 aggccttgcc cgcgccgtac tggccgttcc aggggctgga gctgatgccg
gtcatcgacg 540 agacgttgat cacggcgccg cggtcctggg cggcaaagat
ccgcatgtag tggtggaagc 600 acttgaggaa ggtcacgacg ttgacgttga
tcatggcctc gtgcttctcc cagggggtgt 660 cctggatctt accgaagctg
tgcaggcagg ccacgtagct catgaagccc atgtccaggc 720 cctcggtcgc
ggcgaagacg gtctcggcag cgccgggctg gctaaagtcg gcgcgcacga 780
ccttggtctc cacgccgtag gtctcgcgga tctcgcctgc gagcacgttc agcttctcct
840 cgcgacggtc gaccatgacg acgttcatgc cgccggcggc gatcttctcg
cagaacgcct 900 tgccgacgcc ctcggtcgcg cccaggatca ggccccactc
accgtacttc tccctcaggt 960 tcatatgtat atctccttct tatacttaac
taatatacta agatggggaa ttgttatccg 1020 ctcacaattc ccctatagtg
agtcgtatta atttcgatta tgcggccgtg tacaatacga 1080 ttactttctg
ttcgacttaa gcattataag ctttcagaac tgtgtcgggc gcatcaccgc 1140
atcaatgccg ccatcaatga cgatctgcgc gccatgcaca tagcttgcgg ccgggctcat
1200 caaaaaggcg atgaccgacg ccatctcgga cggctcggca cggcggccca
tgggaggaac 1260 gaacttggca atggattcgc catagcgcgg gtcctgcagg
cccgcctgca gcaagggagt 1320 ctcggttgca ccgggggcga tggtgttcag
gcgcacgcca gcctcgcccc aggcggcggc 1380 gcgtttgcgc acagccaccg
tcaaagcatt cttgctgccc gcataggcca gatttccgcc 1440 ctgctctccc
gcatgttcga caatggcgcg ggccttggct tcctcgccgg cttccagtgc 1500
cagcgccagt gggttcttgt caaaagccag atgcgcggaa gccacggacg agatgacgac
1560 ggctgcgggc tgatggcctt ttttcagcgc tggcaaaaag gcatccatca
gctcggtcgc 1620 gccaaaataa ttgaccgaaa ccacattgcc aagcaccttg
gtctgcggtc ccaggccggc 1680 gcacagcacc aggccgtcca tgcccttgct
gcacttcgcc agtacatcgg caatcgcctg 1740 ctttcgacct tcggccgtcg
agagatcggc aatcacttcc gcatcgcgta tatcgatgcc 1800 tacgatctgg
tgaccggccg cctccaggac cttgcgcgta gccgcaccaa tgccggtggc 1860
gcagccgctt atcacgatga tggacatgta tatctccttt cacgaattct cagccgcctt
1920 cttgaacttg gcggcctctt ccgaaccgcc ggtggcattg cccttcgagt
aggaatgcgc 1980 gccggtgccg gcaagagcgc cgccctgcac gatgaggtat
tcgtcgcgga tcggacggcc 2040 ctcgaagaag cactccagga tctcgcgggt
gcccgccgca taacgcgcct gcgcggtcag 2100 cgtggtgccg gagatgtgcg
gggtcatgcc gttatagggc atcgtccgcc aggggtggtc 2160 cttcggcgcc
ggctgcggga accacacgtc gccggcatag ccggccagcc ggccggattc 2220
gagcgcacgt gccacggcat cgcggtcgca cagcttgccg cgggcggtgt tgacgatgta
2280 ggcgccacgc ttgaacagct tcagcgtctc gtcattgatc atgtgctcgg
tttcggggtg 2340 cagcgggcag ttcagcgtca ccacgtcgca aaccggatac
atgtcctcgc gggtcgcgtg 2400 ccaggtgagg ttgagctcct tctcgaccga
ttccggcagg cggtgacggt cggtgtagtg 2460 caggtgcacg tcgaacggcg
ccagacggcg cagcaccgcg agaccgatgc ggccggcggc 2520 cacggtgccg
acatgcatcg cctcgaggtc gtaggcgtgg gagacgcagt cggcgatgtt 2580
ccagccgccc ttccgcgccc attcgtgcga gggcagatag ttgcgcacca gcgacaggat
2640 catcatcacc acatgctcgg cgacgctgat cgagttgcag taggtgactt
ccgccacggt 2700 gacgttgcgg tcgatagccg actgaagatc gacgtggtcg
gaaccgatgc cggcggtgag 2760 cgcgagcttc aggttcttgg ccttggcgat
gcgctcgggc gtcagatagg ccggccagaa 2820 gggctgggag atgacgacat
ccgcatcgac cagctcgcgc tcgaacaccg agtcggggcc 2880 gtccttgtcg
gaggtcacga ccagggtgtg gccgttggat tcgagatatt cgcgcaggcc 2940
gagctcgccg gagacggagc cgagcaactg cccgggcgtg aagtcgatgg ccttcggcgt
3000 cggcaagatc tggccgcccg gatagtggtc gatcttcgga agatcgtcgc
gggcataggt 3060 cttcgggtag ccgtcgaccg gatcatcgta aagaacgcac
aggacctttg ccatcatatg 3120 tatatctcct tcttaaagtt aaacaaaatt
atttctagag gggaattgtt atccgctcac 3180 aattccccta tagtgagtcg
tattaatttc gcgggatcga gatctcgatc ctctacgccg 3240 gacgcatcgt
ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 3300
acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
3360 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
ttgcatgcac 3420 cattccttgc ggcggcggtg ctcaacggcc tcaacctact
actgggctgc ttcctaatgc 3480 aggagtcgca taagggagag cgtcgagatc
ccggacacca tcgaatggcg caaaaccttt 3540 cgcggtatgg catgatagcg
cccggaagag agtcaattca gggtggtgaa tgtgaaacca 3600 gtaacgttat
acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 3660
gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
3720 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
gtcgttgctg 3780 attggcgttg ccacctccag tctggccctg cacgcgccgt
cgcaaattgt cgcggcgatt 3840 aaatctcgcg ccgatcaact gggtgccagc
gtggtggtgt cgatggtaga acgaagcggc 3900 gtcgaagcct gtaaagcggc
ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 3960 attaactatc
cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 4020
ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
4080 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
gcaaatcgcg 4140 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
gtctggctgg ctggcataaa 4200 tatctcactc gcaatcaaat tcagccgata
gcggaacggg aaggcgactg gagtgccatg 4260 tccggttttc aacaaaccat
gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 4320 gttgccaacg
atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 4380
gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
4440 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
cgtggaccgc 4500 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
agctgttgcc cgtctcactg 4560 gtgaaaagaa aaaccaccct ggcgcccaat
acgcaaaccg cctctccccg cgcgttggcc 4620 gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 4680 cgcaattaat
gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 4740
agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
4800 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
tctgggtcat 4860 tttcggcgag gaccgctttc gctggagcgc gacgatgatc
ggcctgtcgc ttgcggtatt 4920 cggaatcttg cacgccctcg ctcaagcctt
cgtcactggt cccgccacca aacgtttcgg 4980 cgagaagcag gccattatcg
ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 5040 gtcgttgagg
acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 5100
gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
5160 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
cagcgccctg 5220 caccattatg ttccggatct gcatcgcagg atgctgctgg
ctaccctgtg gaacacctac 5280 atctgtatta acgaagcgct ggcattgacc
ctgagtgatt tttctctggt cccgccgcat 5340 ccataccgcc agttgtttac
cctcacaacg ttccagtaac cgggcatgtt catcatcagt 5400 aacccgtatc
gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 5460
tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
5520 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg
acgcggatga 5580 acaggcagac atctgtgaat cgcttcacga ccacgctgat
gagctttacc gcagctgcct 5640 cgcgcgtttc ggtgatgacg gtgaaaacct
ctgacacatg cagctcccgg agacggtcac 5700 agcttgtctg taagcggatg
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 5760 tggcgggtgt
cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 5820
cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
5880 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
tcctcgctca 5940 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
atcagctcac tcaaaggcgg 6000 taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc 6060 agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6120 cccctgacga
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 6180
tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
6240 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
ctttctcata 6300 gctcacgctg taggtatctc agttcggtgt aggtcgttcg
ctccaagctg ggctgtgtgc 6360 acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca 6420 acccggtaag acacgactta
tcgccactgg cagcagccac tggtaacagg attagcagag 6480 cgaggtatgt
aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6540
gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
6600 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
gtttgcaagc 6660 agcagattac gcgcagaaaa aaaggatctc aagaagatcc
tttgatcttt tctacggggt 6720 ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa 6780 ggatcttcac ctagatcctt
ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6840 atgagtaaac
ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6900
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
6960 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
cgctcaccgg 7020 ctccagattt atcagcaata aaccagccag ccggaagggc
cgagcgcaga agtggtcctg 7080 caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt 7140 cgccagttaa tagtttgcgc
aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 7200 cgtcgtttgg
tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7260
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7320 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
cttactgtca 7380 tgccatccgt aagatgcttt tctgtgactg gtgagtactc
aaccaagtca ttctgagaat 7440 agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaat acgggataat accgcgccac 7500 atagcagaac tttaaaagtg
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 7560 ggatcttacc
gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 7620
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
7680 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
ctttttcaat 7740 attattgaag catttatcag ggttattgtc tcatgagcgg
atacatattt gaatgtattt 7800 agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgaaattg 7860 taaacgttaa tattttgtta
aaattcgcgt taaatttttg ttaaatcagc tcatttttta 7920 accaataggc
cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 7980
tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
8040 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
ccctaatcaa 8100 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
ccctaaaggg agcccccgat 8160 ttagagcttg acggggaaag ccggcgaacg
tggcgagaaa ggaagggaag aaagcgaaag 8220 gagcgggcgc tagggcgctg
gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 8280 ccgcgcttaa
tgcgccgcta cagggcgcgt cccattcgcc a 8321 <210> SEQ ID NO 64
<211> LENGTH: 8190 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: pF(G)r7(A)r3 <400> SEQUENCE: 64 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gctcagaact 180 gtgtcgggcg catcaccgca tcaatgccgc catcaatgac
gatctgcgcg ccatgcacat 240 agcttgcggc cgggctcatc aaaaaggcga
tgaccgacgc catctcggac ggctcggcac 300 ggcggcccat gggaggaacg
aacttggcaa tggattcgcc atagcgcggg tcctgcaggc 360 ccgcctgcag
caagggagtc tcggttgcac cgggggcgat ggtgttcagg cgcacgccag 420
cctcgcccca ggcggcggcg cgtttgcgca cagccaccgt caaagcattc ttgctgcccg
480 cataggccag atttccgccc tgctctcccg catgttcgac aatggcgcgg
gccttggctt 540 cctcgccggc ttccagtgcc agcgccagtg ggttcttgtc
aaaagccaga tgcgcggaag 600 ccacggacga gatgacgacg gctgcgggct
gatggccttt tttcagcgct ggcaaaaagg 660 catccatcag ctcggtcgcg
ccaaaataat tgaccgaaac cacattgcca agcaccttgg 720 tctgcggtcc
caggccggcg cacagcacca ggccgtccat gcccttgctg cacttcgcca 780
gtacatcggc aatcgcctgc tttcgacctt cggccgtcga gagatcggca atcacttccg
840 catcgcgtat atcgatgcct acgatctggt gaccggccgc ctccaggacc
ttgcgcgtag 900 cggcaccaat gccggtggcg cagccgctta tcacgatgat
ggacatgtat atctccttaa 960 gcttctagtc gcggtagaac gaccccatgt
agcggatgta ctcgtcctcg gtgtggtttg 1020 ccttccagtc gtggacggag
tccttgttgc gctggccggc gatgacggag agctccttac 1080 ccagcttctc
aaaggcctcg tcaacgcact cctcgggggt gagggcgatc ttcatgacgg 1140
cctcgccctg cgggccgccg gggaggttgg acagcaggct gggggttagg gtggtgccga
1200 gggtgatgac ctcgacgtcg acgccggtgc cctcgcactc gcaggccacg
gcctcggtca 1260 tcttgaggat gaaggccttg cccgcgccgt actggccgtt
ccaggggctg gagctgatgc 1320 cggtcatcga cgagacgttg atcacggcgc
cgcggtcctg ggcggcaaag atccgcatgt 1380 agtggtggaa gcacttgagg
aaggtcacga cgttgacgtt gatcatggcc tcgtgcttct 1440 cccagggggt
gtcctggatc ttaccgaagc tgtgcaggca ggccacgtag ctcatgaagc 1500
ccatgtccag gccctcggtc gcggcgaaga cggtctcggc agcgccgggc tggctaaagt
1560 cggcgcgcac gaccttggtc tccacgccgt aggtctcgcg gatctcgcct
gcgagcacgt 1620 tcagcttctc ctcgcgacgg gcgaccatga cgacgttcat
gccgccggcg gcgatcttct 1680 cgcagaacgc cttgccgacg ccctcggtcg
cgcccaggat caggccccac tcaccgtact 1740 tctccctcag gttcatgtat
atctcctttc acgaattctc agccgccttc ttgaacttgg 1800 cggcctcttc
cgaaccgccg gtggcattgc ccttcgagta ggaatgcgcg ccggtgccgg 1860
caagagcgcc gccctgcacg atgaggtatt cgtcgcggat cggacggccc tcgaagaagc
1920 actccaggat ctcgcgggtg cccgccgcat aacgcgcctg cgcggtcagc
gtggtgccgg 1980 agatgtgcgg ggtcatgccg ttatagggca tcgtccgcca
ggggtggtcc ttcggcgccg 2040 gctgcgggaa ccacacgtcg ccggcatagc
cggccagccg gccggattcg agcgcacgtg 2100 ccacggcatc gcggtcgcac
agcttgccgc gggcggtgtt gacgatgtag gcgccacgct 2160 tgaacagctt
cagcgtctcg tcattgatca tgtgctcggt ttcggggtgc agcgggcagt 2220
tcagcgtcac cacgtcgcaa accggataca tgtcctcgcg ggtcgcgtgc caggtgaggt
2280 tgagctcctt ctcgaccgat tccggcaggc ggtgacggcc ggtgtagtgc
aggtgcacgt 2340 cgaacggcgc cagacggcgc agcaccgcga gaccgatgcg
gccggcggcc acggtgccga 2400 catgcatcgc ctcgaggtcg taggcgtggg
agacgcagtc ggcgatgttc cagccgccct 2460 tccgcgccca ttcgtgcgag
ggcagatagt tgcgcaccag cgacaggatc atcatcacca 2520 catgctcggc
gacgctgatc gagttgcagt aggtgacttc cgccacggtg acgttgcggt 2580
cgatagccga ctgaagatcg acgtggtcgg aaccgatgcc ggcggtgagc gcgagcttca
2640 ggttcttggc cttggcgatg cgctcgggcg tcagataggc cggccagaag
ggctgggaga 2700 tgacgacatc cgcatcgacc agctcgcgct cgaacaccga
gtcggggccg tccttgtcgg 2760 aggtcacgac cagggtgtgg ccgttggatt
cgagatattc gcgcaggccg agctcgccgg 2820 agacggagcc gagcaactgc
ccgggcgtga agtcgatggc cttcggcgtc ggcaagatct 2880 ggccgcccgg
atagtggtcg atcttcggaa gatcgtcgcg ggcataggtc ttcgggtagc 2940
cgtcgaccgg atcatcgtaa agaacgcaca ggacctttgc catcatatgt atatctcctt
3000 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
attcccctat 3060 agtgagtcgt attaatttcg cgggatcgag atctcgatcc
tctacgccgg acgcatcgtg 3120 gccggcatca ccggcgccac aggtgcggtt
gctggcgcct atatcgccga catcaccgat 3180 ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg 3240 gcaggccccg
tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg 3300
gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
3360 aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
gcggtatggc 3420 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
gtgaaaccag taacgttata 3480 cgatgtcgca gagtatgccg gtgtctctta
tcagaccgtt tcccgcgtgg tgaaccaggc 3540 cagccacgtt tctgcgaaaa
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 3600 cattcccaac
cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 3660
cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
3720 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
tcgaagcctg 3780 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
gggctgatca ttaactatcc 3840 gctggatgac caggatgcca ttgctgtgga
agctgcctgc actaatgttc cggcgttatt 3900 tcttgatgtc tctgaccaga
cacccatcaa cagtattatt ttctcccatg aagacggtac 3960 gcgactgggc
gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 4020
cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
4080 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
ccggttttca 4140 acaaaccatg caaatgctga atgagggcat cgttcccact
gcgatgctgg ttgccaacga 4200 tcagatggcg ctgggcgcaa tgcgcgccat
taccgagtcc gggctgcgcg ttggtgcgga 4260 tatctcggta gtgggatacg
acgataccga agacagctca tgttatatcc cgccgttaac 4320 caccatcaaa
caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 4380
ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
4440 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
attcattaat 4500 gcagctggca cgacaggttt cccgactgga aagcgggcag
tgagcgcaac gcaattaatg 4560 taagttagct cactcattag gcaccgggat
ctcgaccgat gcccttgaga gccttcaacc 4620 cagtcagctc cttccggtgg
gcgcggggca tgactatcgt cgccgcactt atgactgtct 4680 tctttatcat
gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg 4740
accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
4800 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
gagaagcagg 4860 ccattatcgc cggcatggcg gccccacggg tgcgcatgat
cgtgctcctg tcgttgagga 4920 cccggctagg ctggcggggt tgccttactg
gttagcagaa tgaatcaccg atacgcgagc 4980 gaacgtgaag cgactgctgc
tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct 5040 tcggtttccg
tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt 5100
tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
5160 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
cataccgcca 5220 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
atcatcagta acccgtatcg 5280 tgagcatcct ctctcgtttc atcggtatca
ttacccccat gaacagaaat cccccttaca 5340 cggaggcatc agtgaccaaa
caggaaaaaa ccgcccttaa catggcccgc tttatcagaa 5400 gccagacatt
aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca 5460
tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg
5520 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
gcttgtctgt 5580 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
agcgggtgtt ggcgggtgtc 5640 ggggcgcagc catgacccag tcacgtagcg
atagcggagt gtatactggc ttaactatgc 5700 ggcatcagag cagattgtac
tgagagtgca ccatatatgc ggtgtgaaat accgcacaga 5760 tgcgtaagga
gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 5820
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
5880 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
gcaaaaggcc 5940 aggaaccgta aaaaggccgc gttgctggcg tttttccata
ggctccgccc ccctgacgag 6000 catcacaaaa atcgacgctc aagtcagagg
tggcgaaacc cgacaggact ataaagatac 6060 caggcgtttc cccctggaag
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6120 ggatacctgt
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6180
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
6240 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
cccggtaaga 6300 cacgacttat cgccactggc agcagccact ggtaacagga
ttagcagagc gaggtatgta 6360 ggcggtgcta cagagttctt gaagtggtgg
cctaactacg gctacactag aaggacagta 6420 tttggtatct gcgctctgct
gaagccagtt accttcggaa aaagagttgg tagctcttga 6480 tccggcaaac
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 6540
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
6600 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
gatcttcacc 6660 tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata tgagtaaact 6720 tggtctgaca gttaccaatg cttaatcagt
gaggcaccta tctcagcgat ctgtctattt 6780 cgttcatcca tagttgcctg
actccccgtc gtgtagataa ctacgatacg ggagggctta 6840 ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 6900
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
6960 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
gccagttaat 7020 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
tgtcacgctc gtcgtttggt 7080 atggcttcat tcagctccgg ttcccaacga
tcaaggcgag ttacatgatc ccccatgttg 7140 tgcaaaaaag cggttagctc
cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 7200 gtgttatcac
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 7260
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
7320 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
tagcagaact 7380 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag gatcttaccg 7440 ctgttgagat ccagttcgat gtaacccact
cgtgcaccca actgatcttc agcatctttt 7500 actttcacca gcgtttctgg
gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 7560 ataagggcga
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 7620
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
7680 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt
aaacgttaat 7740 attttgttaa aattcgcgtt aaatttttgt taaatcagct
cattttttaa ccaataggcc 7800 gaaatcggca aaatccctta taaatcaaaa
gaatagaccg agatagggtt gagtgttgtt 7860 ccagtttgga acaagagtcc
actattaaag aacgtggact ccaacgtcaa agggcgaaaa 7920 accgtctatc
agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 7980
tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
8040 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
agcgggcgct 8100 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
ccacacccgc cgcgcttaat 8160 gcgccgctac agggcgcgtc ccattcgcca 8190
<210> SEQ ID NO 65 <211> LENGTH: 8190 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: pF(G)r7(S)r3 <400> SEQUENCE:
65 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gctcagaact 180 gtgtcgggcg catcaccgca
tcaatgccgc catcaatgac gatctgcgcg ccatgcacat 240 agcttgcggc
cgggctcatc aaaaaggcga tgaccgacgc catctcggac ggctcggcac 300
ggcggcccat gggaggaacg aacttggcaa tggattcgcc atagcgcggg tcctgcaggc
360 ccgcctgcag caagggagtc tcggttgcac cgggggcgat ggtgttcagg
cgcacgccag 420 cctcgcccca ggcggcggcg cgtttgcgca cagccaccgt
caaagcattc ttgctgcccg 480 cataggccag atttccgccc tgctctcccg
catgttcgac aatggcgcgg gccttggctt 540 cctcgccggc ttccagtgcc
agcgccagtg ggttcttgtc aaaagccaga tgcgcggaag 600 ccacggacga
gatgacgacg gctgcgggct gatggccttt tttcagcgct ggcaaaaagg 660
catccatcag ctcggtcgcg ccaaaataat tgaccgaaac cacattgcca agcaccttgg
720 tctgcggtcc caggccggcg cacagcacca ggccgtccat gcccttgctg
cacttcgcca 780 gtacatcggc aatcgcctgc tttcgacctt cggccgtcga
gagatcggca atcacttccg 840 catcgcgtat atcgatgcct acgatctggt
gaccggccgc ctccaggacc ttgcgcgtag 900 cggcaccaat gccggtggcg
cagccgctta tcacgatgat ggacatgtat atctccttaa 960 gcttctagtc
gcggtagaac gaccccatgt agcggatgta ctcgtcctcg gtgtggtttg 1020
ccttccagtc gtggacggag tccttgttgc gctggccggc gatgacggag agctccttac
1080 ccagcttctc aaaggcctcg tcaacgcact cctcgggggt gagggcgatc
ttcatgacgg 1140 cctcgccctg cgggccgccg gggaggttgg acagcaggct
gggggttagg gtggtgccga 1200 gggtgatgac ctcgacgtcg acgccggtgc
cctcgcactc gcaggccacg gcctcggtca 1260 tcttgaggat gaaggccttg
cccgcgccgt actggccgtt ccaggggctg gagctgatgc 1320 cggtcatcga
cgagacgttg atcacggcgc cgcggtcctg ggcggcaaag atccgcatgt 1380
agtggtggaa gcacttgagg aaggtcacga cgttgacgtt gatcatggcc tcgtgcttct
1440 cccagggggt gtcctggatc ttaccgaagc tgtgcaggca ggccacgtag
ctcatgaagc 1500 ccatgtccag gccctcggtc gcggcgaaga cggtctcggc
agcgccgggc tggctaaagt 1560 cggcgcgcac gaccttggtc tccacgccgt
aggtctcgcg gatctcgcct gcgagcacgt 1620 tcagcttctc ctcgcgacgg
ctgaccatga cgacgttcat gccgccggcg gcgatcttct 1680 cgcagaacgc
cttgccgacg ccctcggtcg cgcccaggat caggccccac tcaccgtact 1740
tctccctcag gttcatgtat atctcctttc acgaattctc agccgccttc ttgaacttgg
1800 cggcctcttc cgaaccgccg gtggcattgc ccttcgagta ggaatgcgcg
ccggtgccgg 1860 caagagcgcc gccctgcacg atgaggtatt cgtcgcggat
cggacggccc tcgaagaagc 1920 actccaggat ctcgcgggtg cccgccgcat
aacgcgcctg cgcggtcagc gtggtgccgg 1980 agatgtgcgg ggtcatgccg
ttatagggca tcgtccgcca ggggtggtcc ttcggcgccg 2040 gctgcgggaa
ccacacgtcg ccggcatagc cggccagccg gccggattcg agcgcacgtg 2100
ccacggcatc gcggtcgcac agcttgccgc gggcggtgtt gacgatgtag gcgccacgct
2160 tgaacagctt cagcgtctcg tcattgatca tgtgctcggt ttcggggtgc
agcgggcagt 2220 tcagcgtcac cacgtcgcaa accggataca tgtcctcgcg
ggtcgcgtgc caggtgaggt 2280 tgagctcctt ctcgaccgat tccggcaggc
ggtgacggcc ggtgtagtgc aggtgcacgt 2340 cgaacggcgc cagacggcgc
agcaccgcga gaccgatgcg gccggcggcc acggtgccga 2400 catgcatcgc
ctcgaggtcg taggcgtggg agacgcagtc ggcgatgttc cagccgccct 2460
tccgcgccca ttcgtgcgag ggcagatagt tgcgcaccag cgacaggatc atcatcacca
2520 catgctcggc gacgctgatc gagttgcagt aggtgacttc cgccacggtg
acgttgcggt 2580 cgatagccga ctgaagatcg acgtggtcgg aaccgatgcc
ggcggtgagc gcgagcttca 2640 ggttcttggc cttggcgatg cgctcgggcg
tcagataggc cggccagaag ggctgggaga 2700 tgacgacatc cgcatcgacc
agctcgcgct cgaacaccga gtcggggccg tccttgtcgg 2760 aggtcacgac
cagggtgtgg ccgttggatt cgagatattc gcgcaggccg agctcgccgg 2820
agacggagcc gagcaactgc ccgggcgtga agtcgatggc cttcggcgtc ggcaagatct
2880 ggccgcccgg atagtggtcg atcttcggaa gatcgtcgcg ggcataggtc
ttcgggtagc 2940 cgtcgaccgg atcatcgtaa agaacgcaca ggacctttgc
catcatatgt atatctcctt 3000 cttaaagtta aacaaaatta tttctagagg
ggaattgtta tccgctcaca attcccctat 3060 agtgagtcgt attaatttcg
cgggatcgag atctcgatcc tctacgccgg acgcatcgtg 3120 gccggcatca
ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat 3180
ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
3240 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
attccttgcg 3300 gcggcggtgc tcaacggcct caacctacta ctgggctgct
tcctaatgca ggagtcgcat 3360 aagggagagc gtcgagatcc cggacaccat
cgaatggcgc aaaacctttc gcggtatggc 3420 atgatagcgc ccggaagaga
gtcaattcag ggtggtgaat gtgaaaccag taacgttata 3480 cgatgtcgca
gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 3540
cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
3600 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
ttggcgttgc 3660 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
gcggcgatta aatctcgcgc 3720 cgatcaactg ggtgccagcg tggtggtgtc
gatggtagaa cgaagcggcg tcgaagcctg 3780 taaagcggcg gtgcacaatc
ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 3840 gctggatgac
caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 3900
tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
3960 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
tgttagcggg 4020 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
tggcataaat atctcactcg 4080 caatcaaatt cagccgatag cggaacggga
aggcgactgg agtgccatgt ccggttttca 4140 acaaaccatg caaatgctga
atgagggcat cgttcccact gcgatgctgg ttgccaacga 4200 tcagatggcg
ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 4260
tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
4320 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
tgctgcaact 4380 ctctcagggc caggcggtga agggcaatca gctgttgccc
gtctcactgg tgaaaagaaa 4440 aaccaccctg gcgcccaata cgcaaaccgc
ctctccccgc gcgttggccg attcattaat 4500 gcagctggca cgacaggttt
cccgactgga aagcgggcag tgagcgcaac gcaattaatg 4560 taagttagct
cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc 4620
cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
4680 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
ttcggcgagg 4740 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc 4800 acgccctcgc tcaagccttc gtcactggtc
ccgccaccaa acgtttcggc gagaagcagg 4860 ccattatcgc cggcatggcg
gccccacggg tgcgcatgat cgtgctcctg tcgttgagga 4920 cccggctagg
ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc 4980
gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
5040 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
accattatgt 5100 tccggatctg catcgcagga tgctgctggc taccctgtgg
aacacctaca tctgtattaa 5160 cgaagcgctg gcattgaccc tgagtgattt
ttctctggtc ccgccgcatc cataccgcca 5220 gttgtttacc ctcacaacgt
tccagtaacc gggcatgttc atcatcagta acccgtatcg 5280 tgagcatcct
ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca 5340
cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
5400 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
caggcagaca 5460 tctgtgaatc gcttcacgac cacgctgatg agctttaccg
cagctgcctc gcgcgtttcg 5520 gtgatgacgg tgaaaacctc tgacacatgc
agctcccgga gacggtcaca gcttgtctgt 5580 aagcggatgc cgggagcaga
caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 5640 ggggcgcagc
catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 5700
ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga
5760 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
tgactcgctg 5820 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
caaaggcggt aatacggtta 5880 tccacagaat caggggataa cgcaggaaag
aacatgtgag caaaaggcca gcaaaaggcc 5940 aggaaccgta aaaaggccgc
gttgctggcg tttttccata ggctccgccc ccctgacgag 6000 catcacaaaa
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6060
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
6120 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
ctcacgctgt 6180 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
gctgtgtgca cgaacccccc 6240 gttcagcccg accgctgcgc cttatccggt
aactatcgtc ttgagtccaa cccggtaaga 6300 cacgacttat cgccactggc
agcagccact ggtaacagga ttagcagagc gaggtatgta 6360 ggcggtgcta
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 6420
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
6480 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
gcagattacg 6540 cgcagaaaaa aaggatctca agaagatcct ttgatctttt
ctacggggtc tgacgctcag 6600 tggaacgaaa actcacgtta agggattttg
gtcatgagat tatcaaaaag gatcttcacc 6660 tagatccttt taaattaaaa
atgaagtttt aaatcaatct aaagtatata tgagtaaact 6720 tggtctgaca
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 6780
cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
6840 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
tccagattta 6900 tcagcaataa accagccagc cggaagggcc gagcgcagaa
gtggtcctgc aactttatcc 6960 gcctccatcc agtctattaa ttgttgccgg
gaagctagag taagtagttc gccagttaat 7020 agtttgcgca acgttgttgc
cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 7080 atggcttcat
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 7140
tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
7200 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
gccatccgta 7260 agatgctttt ctgtgactgg tgagtactca accaagtcat
tctgagaata gtgtatgcgg 7320 cgaccgagtt gctcttgccc ggcgtcaata
cgggataata ccgcgccaca tagcagaact 7380 ttaaaagtgc tcatcattgg
aaaacgttct tcggggcgaa aactctcaag gatcttaccg 7440 ctgttgagat
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 7500
actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
7560 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
ttattgaagc 7620 atttatcagg gttattgtct catgagcgga tacatatttg
aatgtattta gaaaaataaa 7680 caaatagggg ttccgcgcac atttccccga
aaagtgccac ctgaaattgt aaacgttaat 7740 attttgttaa aattcgcgtt
aaatttttgt taaatcagct cattttttaa ccaataggcc 7800 gaaatcggca
aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 7860
ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
7920 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
ttttttgggg 7980 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
gcccccgatt tagagcttga 8040 cggggaaagc cggcgaacgt ggcgagaaag
gaagggaaga aagcgaaagg agcgggcgct 8100 agggcgctgg caagtgtagc
ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 8160 gcgccgctac
agggcgcgtc ccattcgcca 8190 <210> SEQ ID NO 66 <211>
LENGTH: 7910 <212> TYPE: DNA <213> ORGANISM: artificial
<220> FEATURE: <223> OTHER INFORMATION: p3T7(A)rG
<400> SEQUENCE: 66 atccggatat agttcctcct ttcagcaaaa
aacccctcaa gacccgttta gaggccccaa 60 ggggttatgc tagttattgc
tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120 tgttagcagc
cggatctcag tggtggtggt ggtggtgctc gagttaaccg cggcctgcct 180
ggaatgaagg atattgtgtc ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc
240 tggcttcctt cgaagcaagc caggctgcta ctgcggcgat ctcctccggt
tcgccgatat 300 atcccattgg aatcatgctt tctacatcag ctttctgttt
agggtcagcg aatttttcag 360 cattgattgg cgtgttgatc gcacctggcc
caatattatt gacgcgaatg cccttcggcg 420 cgtattccaa cgctaatgtt
tctgtcatca gctttatccc gcctttactt gccgcatagt 480 ggacaaataa
cggccaagga atcacttcgt gcacactgga catgttaatg acatttccct 540
tgatatcgtt ttctacgaaa tatttaatcg cttcacggct tcctaaaaag gcacccgtta
600 agttcgtgcc gatgacttta tcccaatcct tgagcggcat ttcgtgagat
ggcacaggat 660 tttcaagacc ggcattatta atcataatat cgagtgtgcc
gaactcctta attgccgttt 720 gcacgatatt ttttacatct tcctctttcg
tgacatctcc ttggacgaca acagcttcac 780 cgcccgcctt gatgacctct
tcttttacct cgttcggatc ttgtttatta ctataatagt 840 tgataaccac
ttttgcctgc tccttgccga agcgaatggc catcgccttt ccgagccctg 900
aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacatg tatatctcct
960 tgcggccgcc tagtcgcggt agaacgaccc catgtagcgg atgtactcgt
cctcggtgtg 1020 gtttgccttc cagtcgtgga cggagtcctt gttgcgctgg
ccggcgatga cggagagctc 1080 cttacccagc ttctcaaagg cctcgtcaac
gcactcctcg ggggtgaggg cgatcttcat 1140 gacggcctcg ccctgcgggc
cgccggggag gttggacagc aggctggggg ttagggtggt 1200 gccgagggtg
atgacctcga cgtcgacgcc ggtgccctcg cactcgcagg ccacggcctc 1260
ggtcatcttg aggatgaagg ccttgcccgc gccgtactgg ccgttccagg ggctggagct
1320 gatgccggtc atcgacgaga cgttgatcac ggcgccgcgg tcctgggcgg
caaagatccg 1380 catgtagtgg tggaagcact tgaggaaggt cacgacgttg
acgttgatca tggcctcgtg 1440 cttctcccag ggggtgtcct ggatcttacc
gaagctgtgc aggcaggcca cgtagctcat 1500 gaagcccatg tccaggccct
cggtcgcggc gaagacggtc tcggcagcgc cgggctggct 1560 aaagtcggcg
cgcacgacct tggtctccac gccgtaggtc tcgcggatct cgcctgcgag 1620
cacgttcagc ttctcctcgc gacgggcgac catgacgacg ttcatgccgc cggcggcgat
1680 cttctcgcag aacgccttgc cgacgccctc ggtcgcgccc aggatcaggc
cccactcacc 1740 gtacttctcc ctcaggttca tatgtatatc tccttcttat
acttaactaa tatactaaga 1800 tggggaattg ttatccgctc acaattcccc
tatagtgagt cgtattaatt tcgattatgc 1860 ggccgtgtac aatacgatta
ctttctgttc gacttaagca ttataagctt gtcgacggag 1920 ctcgaattct
cagaactgtg tcgggcgcat caccgcatca atgccgccat caatgacgat 1980
ctgcgcgcca tgcacatagc ttgcggccgg gctcatcaaa aaggcgatga ccgacgccat
2040 ctcggacggc tcggcacggc ggcccatggg aggaacgaac ttggcaatgg
attcgccata 2100 gcgcgggtcc tgcaggcccg cctgcagcaa gggagtctcg
gttgcaccgg gggcgatggt 2160 gttcaggcgc acgccagcct cgccccaggc
ggcggcgcgt ttgcgcacag ccaccgtcaa 2220 agcattcttg ctgcccgcat
aggccagatt tccgccctgc tctcccgcat gttcgacaat 2280 ggcgcgggcc
ttggcttcct cgccggcttc cagtgccagc gccagtgggt tcttgtcaaa 2340
agccagatgc gcggaagcca cggacgagat gacgacggct gcgggctgat ggcctttttt
2400 cagcgctggc aaaaaggcat ccatcagctc ggtcgcgcca aaataattga
ccgaaaccac 2460 attgccaagc accttggtct gcggtcccag gccggcgcac
agcaccaggc cgtccatgcc 2520 cttgctgcac ttcgccagta catcggcaat
cgcctgcttt cgaccttcgg ccgtcgagag 2580 atcggcaatc acttccgcat
cgcgtatatc gatgcctacg atctggtgac cggccgcctc 2640 caggaccttg
cgcgtagccg caccaatgcc ggtggcgcag ccgcttatca cgatgatgga 2700
catcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
2760 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
atctcgatcc 2820 tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct 2880 atatcgccga catcaccgat ggggaagatc
gggctcgcca cttcgggctc atgagcgctt 2940 gtttcggcgt gggtatggtg
gcaggccccg tggccggggg actgttgggc gccatctcct 3000 tgcatgcacc
attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 3060
tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
3120 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
ggtggtgaat 3180 gtgaaaccag taacgttata cgatgtcgca gagtatgccg
gtgtctctta tcagaccgtt 3240 tcccgcgtgg tgaaccaggc cagccacgtt
tctgcgaaaa cgcgggaaaa agtggaagcg 3300 gcgatggcgg agctgaatta
cattcccaac cgcgtggcac aacaactggc gggcaaacag 3360 tcgttgctga
ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 3420
gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
3480 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
acgcgtcagt 3540 gggctgatca ttaactatcc gctggatgac caggatgcca
ttgctgtgga agctgcctgc 3600 actaatgttc cggcgttatt tcttgatgtc
tctgaccaga cacccatcaa cagtattatt 3660 ttctcccatg aagacggtac
gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 3720 caaatcgcgc
tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 3780
tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
3840 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
cgttcccact 3900 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
tgcgcgccat taccgagtcc 3960 gggctgcgcg ttggtgcgga tatctcggta
gtgggatacg acgataccga agacagctca 4020 tgttatatcc cgccgttaac
caccatcaaa caggattttc gcctgctggg gcaaaccagc 4080 gtggaccgct
tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 4140
gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
4200 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
aagcgggcag 4260 tgagcgcaac gcaattaatg taagttagct cactcattag
gcaccgggat ctcgaccgat 4320 gcccttgaga gccttcaacc cagtcagctc
cttccggtgg gcgcggggca tgactatcgt 4380 cgccgcactt atgactgtct
tctttatcat gcaactcgta ggacaggtgc cggcagcgct 4440 ctgggtcatt
ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 4500
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
4560 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg
tgcgcatgat 4620 cgtgctcctg tcgttgagga cccggctagg ctggcggggt
tgccttactg gttagcagaa 4680 tgaatcaccg atacgcgagc gaacgtgaag
cgactgctgc tgcaaaacgt ctgcgacctg 4740 agcaacaaca tgaatggtct
tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc 4800 agcgccctgc
accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 4860
aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
4920 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
gggcatgttc 4980 atcatcagta acccgtatcg tgagcatcct ctctcgtttc
atcggtatca ttacccccat 5040 gaacagaaat cccccttaca cggaggcatc
agtgaccaaa caggaaaaaa ccgcccttaa 5100 catggcccgc tttatcagaa
gccagacatt aacgcttctg gagaaactca acgagctgga 5160 cgcggatgaa
caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 5220
cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
5280 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
agggcgcgtc 5340 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
tcacgtagcg atagcggagt 5400 gtatactggc ttaactatgc ggcatcagag
cagattgtac tgagagtgca ccatatatgc 5460 ggtgtgaaat accgcacaga
tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 5520 cctcgctcac
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5580
caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
5640 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
tttttccata 5700 ggctccgccc ccctgacgag catcacaaaa atcgacgctc
aagtcagagg tggcgaaacc 5760 cgacaggact ataaagatac caggcgtttc
cccctggaag ctccctcgtg cgctctcctg 5820 ttccgaccct gccgcttacc
ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5880 tttctcatag
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5940
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
6000 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
ggtaacagga 6060 ttagcagagc gaggtatgta ggcggtgcta cagagttctt
gaagtggtgg cctaactacg 6120 gctacactag aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa 6180 aaagagttgg tagctcttga
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6240 tttgcaagca
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6300
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
6360 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
aaatcaatct 6420 aaagtatata tgagtaaact tggtctgaca gttaccaatg
cttaatcagt gaggcaccta 6480 tctcagcgat ctgtctattt cgttcatcca
tagttgcctg actccccgtc gtgtagataa 6540 ctacgatacg ggagggctta
ccatctggcc ccagtgctgc aatgataccg cgagacccac 6600 gctcaccggc
tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6660
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
6720 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
ggcatcgtgg 6780 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
ttcccaacga tcaaggcgag 6840 ttacatgatc ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg 6900 tcagaagtaa gttggccgca
gtgttatcac tcatggttat ggcagcactg cataattctc 6960 ttactgtcat
gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7020
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
7080 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
tcggggcgaa 7140 aactctcaag gatcttaccg ctgttgagat ccagttcgat
gtaacccact cgtgcaccca 7200 actgatcttc agcatctttt actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc 7260 aaaatgccgc aaaaaaggga
ataagggcga cacggaaatg ttgaatactc atactcttcc 7320 tttttcaata
ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 7380
aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
7440 ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
taaatcagct 7500 cattttttaa ccaataggcc gaaatcggca aaatccctta
taaatcaaaa gaatagaccg 7560 agatagggtt gagtgttgtt ccagtttgga
acaagagtcc actattaaag aacgtggact 7620 ccaacgtcaa agggcgaaaa
accgtctatc agggcgatgg cccactacgt gaaccatcac 7680 cctaatcaag
ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 7740
gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
7800 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
cgcgtaacca 7860 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
ccattcgcca 7910 <210> SEQ ID NO 67 <211> LENGTH: 7891
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: p7(A)T3rG <400>
SEQUENCE: 67 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagttaaccg cggcctgcct 180 ggaatgaagg atattgtgtc
ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc 240 tggcttcctt
cgaagcaagc caggctgcta ctgcggcgat ctcctccggt tcgccgatat 300
atcccattgg aatcatgctt tctacatcag ctttctgttt agggtcagcg aatttttcag
360 cattgattgg cgtgttgatc gcacctggcc caatattatt gacgcgaatg
cccttcggcg 420 cgtattccaa cgctaatgtt tctgtcatca gctttatccc
gcctttactt gccgcatagt 480 ggacaaataa cggccaagga atcacttcgt
gcacactgga catgttaatg acatttccct 540 tgatatcgtt ttctacgaaa
tatttaatcg cttcacggct tcctaaaaag gcacccgtta 600 agttcgtgcc
gatgacttta tcccaatcct tgagcggcat ttcgtgagat ggcacaggat 660
tttcaagacc ggcattatta atcataatat cgagtgtgcc gaactcctta attgccgttt
720 gcacgatatt ttttacatct tcctctttcg tgacatctcc ttggacgaca
acagcttcac 780 cgcccgcctt gatgacctct tcttttacct cgttcggatc
ttgtttatta ctataatagt 840 tgataaccac ttttgcctgc tccttgccga
agcgaatggc catcgccttt ccgagccctg 900 aagcagctcc tgtaatagcg
acgacttttc cttttaaatc cggatacatg tatatctcct 960 tgcggccgct
cagaactgtg tcgggcgcat caccgcatca atgccgccat caatgacgat 1020
ctgcgcgcca tgcacatagc ttgcggccgg gctcatcaaa aaggcgatga ccgacgccat
1080 ctcggacggc tcggcacggc ggcccatggg aggaacgaac ttggcaatgg
attcgccata 1140 gcgcgggtcc tgcaggcccg cctgcagcaa gggagtctcg
gttgcaccgg gggcgatggt 1200 gttcaggcgc acgccagcct cgccccaggc
ggcggcgcgt ttgcgcacag ccaccgtcaa 1260 agcattcttg ctgcccgcat
aggccagatt tccgccctgc tctcccgcat gttcgacaat 1320 ggcgcgggcc
ttggcttcct cgccggcttc cagtgccagc gccagtgggt tcttgtcaaa 1380
agccagatgc gcggaagcca cggacgagat gacgacggct gcgggctgat ggcctttttt
1440 cagcgctggc aaaaaggcat ccatcagctc ggtcgcgcca aaataattga
ccgaaaccac 1500 attgccaagc accttggtct gcggtcccag gccggcgcac
agcaccaggc cgtccatgcc 1560 cttgctgcac ttcgccagta catcggcaat
cgcctgcttt cgaccttcgg ccgtcgagag 1620 atcggcaatc acttccgcat
cgcgtatatc gatgcctacg atctggtgac cggccgcctc 1680 caggaccttg
cgcgtagcgg caccaatgcc ggtggcgcag ccgcttatca cgatgatgga 1740
catcatatgt atatctcctt cttatactta actaatatac taagatgggg aattgttatc
1800 cgctcacaat tcccctatag tgagtcgtat taatttcgat tatgcggccg
tgtacaatac 1860 gattactttc tgttcgactt aagcattata agcttctagt
cgcggtagaa cgaccccatg 1920 tagcggatgt actcgtcctc ggtgtggttt
gccttccagt cgtggacgga gtccttgttg 1980 cgctggccgg cgatgacgga
gagctcctta cccagcttct caaaggcctc gtcaacgcac 2040 tcctcggggg
tgagggcgat cttcatgacg gcctcgccct gcgggccgcc ggggaggttg 2100
gacagcaggc tgggggttag ggtggtgccg agggtgatga cctcgacgtc gacgccggtg
2160 ccctcgcact cgcaggccac ggcctcggtc atcttgagga tgaaggcctt
gcccgcgccg 2220 tactggccgt tccaggggct ggagctgatg ccggtcatcg
acgagacgtt gatcacggcg 2280 ccgcggtcct gggcggcaaa gatccgcatg
tagtggtgga agcacttgag gaaggtcacg 2340 acgttgacgt tgatcatggc
ctcgtgcttc tcccaggggg tgtcctggat cttaccgaag 2400 ctgtgcaggc
aggccacgta gctcatgaag cccatgtcca ggccctcggt cgcggcgaag 2460
acggtctcgg cagcgccggg ctggctaaag tcggcgcgca cgaccttggt ctccacgccg
2520 taggtctcgc ggatctcgcc tgcgagcacg ttcagcttct cctcgcgacg
ggcgaccatg 2580 acgacgttca tgccgccggc ggcgatcttc tcgcagaacg
ccttgccgac gccctcggtc 2640 gcgcccagga tcaggcccca ctcaccgtac
ttctccctca ggttcatatg tatatctcct 2700 tcttaaagtt aaacaaaatt
atttctagag gggaattgtt atccgctcac aattccccta 2760 tagtgagtcg
tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt 2820
ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
2880 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
tgggtatggt 2940 ggcaggcccc gtggccgggg gactgttggg cgccatctcc
ttgcatgcac cattccttgc 3000 ggcggcggtg ctcaacggcc tcaacctact
actgggctgc ttcctaatgc aggagtcgca 3060 taagggagag cgtcgagatc
ccggacacca tcgaatggcg caaaaccttt cgcggtatgg 3120 catgatagcg
cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat 3180
acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
3240 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
gagctgaatt 3300 acattcccaa ccgcgtggca caacaactgg cgggcaaaca
gtcgttgctg attggcgttg 3360 ccacctccag tctggccctg cacgcgccgt
cgcaaattgt cgcggcgatt aaatctcgcg 3420 ccgatcaact gggtgccagc
gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct 3480 gtaaagcggc
ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc 3540
cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
3600 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
gaagacggta 3660 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
gcaaatcgcg ctgttagcgg 3720 gcccattaag ttctgtctcg gcgcgtctgc
gtctggctgg ctggcataaa tatctcactc 3780 gcaatcaaat tcagccgata
gcggaacggg aaggcgactg gagtgccatg tccggttttc 3840 aacaaaccat
gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 3900
atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
3960 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
ccgccgttaa 4020 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
cgtggaccgc ttgctgcaac 4080 tctctcaggg ccaggcggtg aagggcaatc
agctgttgcc cgtctcactg gtgaaaagaa 4140 aaaccaccct ggcgcccaat
acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4200 tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4260
gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac
4320 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
tatgactgtc 4380 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
tctgggtcat tttcggcgag 4440 gaccgctttc gctggagcgc gacgatgatc
ggcctgtcgc ttgcggtatt cggaatcttg 4500 cacgccctcg ctcaagcctt
cgtcactggt cccgccacca aacgtttcgg cgagaagcag 4560 gccattatcg
ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg 4620
acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
4680 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
atgaatggtc 4740 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
cagcgccctg caccattatg 4800 ttccggatct gcatcgcagg atgctgctgg
ctaccctgtg gaacacctac atctgtatta 4860 acgaagcgct ggcattgacc
ctgagtgatt tttctctggt cccgccgcat ccataccgcc 4920 agttgtttac
cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc 4980
gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
5040 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
ctttatcaga 5100 agccagacat taacgcttct ggagaaactc aacgagctgg
acgcggatga acaggcagac 5160 atctgtgaat cgcttcacga ccacgctgat
gagctttacc gcagctgcct cgcgcgtttc 5220 ggtgatgacg gtgaaaacct
ctgacacatg cagctcccgg agacggtcac agcttgtctg 5280 taagcggatg
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 5340
cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
5400 cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
taccgcacag 5460 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
tcctcgctca ctgactcgct 5520 gcgctcggtc gttcggctgc ggcgagcggt
atcagctcac tcaaaggcgg taatacggtt 5580 atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 5640 caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 5700
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
5760 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
tgccgcttac 5820 cggatacctg tccgcctttc tcccttcggg aagcgtggcg
ctttctcata gctcacgctg 5880 taggtatctc agttcggtgt aggtcgttcg
ctccaagctg ggctgtgtgc acgaaccccc 5940 cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag 6000 acacgactta
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6060
aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
6120 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
gtagctcttg 6180 atccggcaaa caaaccaccg ctggtagcgg tggttttttt
gtttgcaagc agcagattac 6240 gcgcagaaaa aaaggatctc aagaagatcc
tttgatcttt tctacggggt ctgacgctca 6300 gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 6360 ctagatcctt
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 6420
ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
6480 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
gggagggctt 6540 accatctggc cccagtgctg caatgatacc gcgagaccca
cgctcaccgg ctccagattt 6600 atcagcaata aaccagccag ccggaagggc
cgagcgcaga agtggtcctg caactttatc 6660 cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa 6720 tagtttgcgc
aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 6780
tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
6840 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
agttggccgc 6900 agtgttatca ctcatggtta tggcagcact gcataattct
cttactgtca tgccatccgt 6960 aagatgcttt tctgtgactg gtgagtactc
aaccaagtca ttctgagaat agtgtatgcg 7020 gcgaccgagt tgctcttgcc
cggcgtcaat acgggataat accgcgccac atagcagaac 7080 tttaaaagtg
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 7140
gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
7200 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
caaaaaaggg 7260 aataagggcg acacggaaat gttgaatact catactcttc
ctttttcaat attattgaag 7320 catttatcag ggttattgtc tcatgagcgg
atacatattt gaatgtattt agaaaaataa 7380 acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgaaattg taaacgttaa 7440 tattttgtta
aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 7500
cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
7560 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
aagggcgaaa 7620 aaccgtctat cagggcgatg gcccactacg tgaaccatca
ccctaatcaa gttttttggg 7680 gtcgaggtgc cgtaaagcac taaatcggaa
ccctaaaggg agcccccgat ttagagcttg 7740 acggggaaag ccggcgaacg
tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 7800 tagggcgctg
gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 7860
tgcgccgcta cagggcgcgt cccattcgcc a 7891 <210> SEQ ID NO 68
<211> LENGTH: 7404 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: pF(G)r7(A) <400> SEQUENCE: 68 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gcaagcttct 180 agtcgcggta gaacgacccc atgtagcgga tgtactcgtc
ctcggtgtgg tttgccttcc 240 agtcgtggac ggagtccttg ttgcgctggc
cggcgatgac ggagagctcc ttacccagct 300 tctcaaaggc ctcgtcaacg
cactcctcgg gggtgagggc gatcttcatg acggcctcgc 360 cctgcgggcc
gccggggagg ttggacagca ggctgggggt tagggtggtg ccgagggtga 420
tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc cacggcctcg gtcatcttga
480 ggatgaaggc cttgcccgcg ccgtactggc cgttccaggg gctggagctg
atgccggtca 540 tcgacgagac gttgatcacg gcgccgcggt cctgggcggc
aaagatccgc atgtagtggt 600 ggaagcactt gaggaaggtc acgacgttga
cgttgatcat ggcctcgtgc ttctcccagg 660 gggtgtcctg gatcttaccg
aagctgtgca ggcaggccac gtagctcatg aagcccatgt 720 ccaggccctc
ggtcgcggcg aagacggtct cggcagcgcc gggctggcta aagtcggcgc 780
gcacgacctt ggtctccacg ccgtaggtct cgcggatctc gcctgcgagc acgttcagct
840 tctcctcgcg acgggcgacc atgacgacgt tcatgccgcc ggcggcgatc
ttctcgcaga 900 acgccttgcc gacgccctcg gtcgcgccca ggatcaggcc
ccactcaccg tacttctccc 960 tcaggttcat gtatatctcc tttcacgaat
tctcagccgc cttcttgaac ttggcggcct 1020 cttccgaacc gccggtggca
ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag 1080 cgccgccctg
cacgatgagg tattcgtcgc ggatcggacg gccctcgaag aagcactcca 1140
ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt cagcgtggtg ccggagatgt
1200 gcggggtcat gccgttatag ggcatcgtcc gccaggggtg gtccttcggc
gccggctgcg 1260 ggaaccacac gtcgccggca tagccggcca gccggccgga
ttcgagcgca cgtgccacgg 1320 catcgcggtc gcacagcttg ccgcgggcgg
tgttgacgat gtaggcgcca cgcttgaaca 1380 gcttcagcgt ctcgtcattg
atcatgtgct cggtttcggg gtgcagcggg cagttcagcg 1440 tcaccacgtc
gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg aggttgagct 1500
ccttctcgac cgattccggc aggcggtgac ggccggtgta gtgcaggtgc acgtcgaacg
1560 gcgccagacg gcgcagcacc gcgagaccga tgcggccggc ggccacggtg
ccgacatgca 1620 tcgcctcgag gtcgtaggcg tgggagacgc agtcggcgat
gttccagccg cccttccgcg 1680 cccattcgtg cgagggcaga tagttgcgca
ccagcgacag gatcatcatc accacatgct 1740 cggcgacgct gatcgagttg
cagtaggtga cttccgccac ggtgacgttg cggtcgatag 1800 ccgactgaag
atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc ttcaggttct 1860
tggccttggc gatgcgctcg ggcgtcagat aggccggcca gaagggctgg gagatgacga
1920 catccgcatc gaccagctcg cgctcgaaca ccgagtcggg gccgtccttg
tcggaggtca 1980 cgaccagggt gtggccgttg gattcgagat attcgcgcag
gccgagctcg ccggagacgg 2040 agccgagcaa ctgcccgggc gtgaagtcga
tggccttcgg cgtcggcaag atctggccgc 2100 ccggatagtg gtcgatcttc
ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga 2160 ccggatcatc
gtaaagaacg cacaggacct ttgccatcat atgtatatct ccttcttaaa 2220
gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
2280 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat
cgtggccggc 2340 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
ccgacatcac cgatggggaa 2400 gatcgggctc gccacttcgg gctcatgagc
gcttgtttcg gcgtgggtat ggtggcaggc 2460 cccgtggccg ggggactgtt
gggcgccatc tccttgcatg caccattcct tgcggcggcg 2520 gtgctcaacg
gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 2580
gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
2640 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
tatacgatgt 2700 cgcagagtat gccggtgtct cttatcagac cgtttcccgc
gtggtgaacc aggccagcca 2760 cgtttctgcg aaaacgcggg aaaaagtgga
agcggcgatg gcggagctga attacattcc 2820 caaccgcgtg gcacaacaac
tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 2880 cagtctggcc
ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 2940
actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
3000 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
atccgctgga 3060 tgaccaggat gccattgctg tggaagctgc ctgcactaat
gttccggcgt tatttcttga 3120 tgtctctgac cagacaccca tcaacagtat
tattttctcc catgaagacg gtacgcgact 3180 gggcgtggag catctggtcg
cattgggtca ccagcaaatc gcgctgttag cgggcccatt 3240 aagttctgtc
tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 3300
aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
3360 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
acgatcagat 3420 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
cgcgttggtg cggatatctc 3480 ggtagtggga tacgacgata ccgaagacag
ctcatgttat atcccgccgt taaccaccat 3540 caaacaggat tttcgcctgc
tggggcaaac cagcgtggac cgcttgctgc aactctctca 3600 gggccaggcg
gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 3660
cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
3720 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
aatgtaagtt 3780 agctcactca ttaggcaccg ggatctcgac cgatgccctt
gagagccttc aacccagtca 3840 gctccttccg gtgggcgcgg ggcatgacta
tcgtcgccgc acttatgact gtcttcttta 3900 tcatgcaact cgtaggacag
gtgccggcag cgctctgggt cattttcggc gaggaccgct 3960 ttcgctggag
cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 4020
tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
4080 tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg
aggacccggc 4140 taggctggcg gggttgcctt actggttagc agaatgaatc
accgatacgc gagcgaacgt 4200 gaagcgactg ctgctgcaaa acgtctgcga
cctgagcaac aacatgaatg gtcttcggtt 4260 tccgtgtttc gtaaagtctg
gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga 4320 tctgcatcgc
aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 4380
gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
4440 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
atcgtgagca 4500 tcctctctcg tttcatcggt atcattaccc ccatgaacag
aaatccccct tacacggagg 4560 catcagtgac caaacaggaa aaaaccgccc
ttaacatggc ccgctttatc agaagccaga 4620 cattaacgct tctggagaaa
ctcaacgagc tggacgcgga tgaacaggca gacatctgtg 4680 aatcgcttca
cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 4740
acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
4800 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
tgtcggggcg 4860 cagccatgac ccagtcacgt agcgatagcg gagtgtatac
tggcttaact atgcggcatc 4920 agagcagatt gtactgagag tgcaccatat
atgcggtgtg aaataccgca cagatgcgta 4980 aggagaaaat accgcatcag
gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 5040 gtcgttcggc
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 5100
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
5160 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
cgagcatcac 5220 aaaaatcgac gctcaagtca gaggtggcga aacccgacag
gactataaag ataccaggcg 5280 tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac 5340 ctgtccgcct ttctcccttc
gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 5400 ctcagttcgg
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5460
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
5520 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
tgtaggcggt 5580 gctacagagt tcttgaagtg gtggcctaac tacggctaca
ctagaaggac agtatttggt 5640 atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc 5700 aaacaaacca ccgctggtag
cggtggtttt tttgtttgca agcagcagat tacgcgcaga 5760 aaaaaaggat
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5820
gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5880 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
aacttggtct 5940 gacagttacc aatgcttaat cagtgaggca cctatctcag
cgatctgtct atttcgttca 6000 tccatagttg cctgactccc cgtcgtgtag
ataactacga tacgggaggg cttaccatct 6060 ggccccagtg ctgcaatgat
accgcgagac ccacgctcac cggctccaga tttatcagca 6120 ataaaccagc
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6180
atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
6240 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
tggtatggct 6300 tcattcagct ccggttccca acgatcaagg cgagttacat
gatcccccat gttgtgcaaa 6360 aaagcggtta gctccttcgg tcctccgatc
gttgtcagaa gtaagttggc cgcagtgtta 6420 tcactcatgg ttatggcagc
actgcataat tctcttactg tcatgccatc cgtaagatgc 6480 ttttctgtga
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6540
agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
6600 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
accgctgttg 6660 agatccagtt cgatgtaacc cactcgtgca cccaactgat
cttcagcatc ttttactttc 6720 accagcgttt ctgggtgagc aaaaacagga
aggcaaaatg ccgcaaaaaa gggaataagg 6780 gcgacacgga aatgttgaat
actcatactc ttcctttttc aatattattg aagcatttat 6840 cagggttatt
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6900
ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg
6960 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
ggccgaaatc 7020 ggcaaaatcc cttataaatc aaaagaatag accgagatag
ggttgagtgt tgttccagtt 7080 tggaacaaga gtccactatt aaagaacgtg
gactccaacg tcaaagggcg aaaaaccgtc 7140 tatcagggcg atggcccact
acgtgaacca tcaccctaat caagtttttt ggggtcgagg 7200 tgccgtaaag
cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 7260
aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
7320 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
taatgcgccg 7380 ctacagggcg cgtcccattc gcca 7404 <210> SEQ ID
NO 69 <211> LENGTH: 7386 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: pFr3 <400> SEQUENCE: 69 atccggatat agttcctcct
ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60 ggggttatgc
tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120
tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc
180 agaactgtgt cgggcgcatc accgcatcaa tgccgccatc aatgacgatc
tgcgcgccat 240 gcacatagct tgcggccggg ctcatcaaaa aggcgatgac
cgacgccatc tcggacggct 300 cggcacggcg gcccatggga ggaacgaact
tggcaatgga ttcgccatag cgcgggtcct 360 gcaggcccgc ctgcagcaag
ggagtctcgg ttgcaccggg ggcgatggtg ttcaggcgca 420 cgccagcctc
gccccaggcg gcggcgcgtt tgcgcacagc caccgtcaaa gcattcttgc 480
tgcccgcata ggccagattt ccgccctgct ctcccgcatg ttcgacaatg gcgcgggcct
540 tggcttcctc gccggcttcc agtgccagcg ccagtgggtt cttgtcaaaa
gccagatgcg 600 cggaagccac ggacgagatg acgacggctg cgggctgatg
gccttttttc agcgctggca 660 aaaaggcatc catcagctcg gtcgcgccaa
aataattgac cgaaaccaca ttgccaagca 720 ccttggtctg cggtcccagg
ccggcgcaca gcaccaggcc gtccatgccc ttgctgcact 780 tcgccagtac
atcggcaatc gcctgctttc gaccttcggc cgtcgagaga tcggcaatca 840
cttccgcatc gcgtatatcg atgcctacga tctggtgacc ggccgcctcc aggaccttgc
900 gcgtagccgc accaatgccg gtggcgcagc cgcttatcac gatgatggac
atgtatatct 960 cctttcacga attctcagcc gccttcttga acttggcggc
ctcttccgaa ccgccggtgg 1020 cattgccctt cgagtaggaa tgcgcgccgg
tgccggcaag agcgccgccc tgcacgatga 1080 ggtattcgtc gcggatcgga
cggccctcga agaagcactc caggatctcg cgggtgcccg 1140 ccgcataacg
cgcctgcgcg gtcagcgtgg tgccggagat gtgcggggtc atgccgttat 1200
agggcatcgt ccgccagggg tggtccttcg gcgccggctg cgggaaccac acgtcgccgg
1260 catagccggc cagccggccg gattcgagcg cacgtgccac ggcatcgcgg
tcgcacagct 1320 tgccgcgggc ggtgttgacg atgtaggcgc cacgcttgaa
cagcttcagc gtctcgtcat 1380 tgatcatgtg ctcggtttcg gggtgcagcg
ggcagttcag cgtcaccacg tcgcaaaccg 1440 gatacatgtc ctcgcgggtc
gcgtgccagg tgaggttgag ctccttctcg accgattccg 1500 gcaggcggtg
acggtcggtg tagtgcaggt gcacgtcgaa cggcgccaga cggcgcagca 1560
ccgcgagacc gatgcggccg gcggccacgg tgccgacatg catcgcctcg aggtcgtagg
1620 cgtgggagac gcagtcggcg atgttccagc cgcccttccg cgcccattcg
tgcgagggca 1680 gatagttgcg caccagcgac aggatcatca tcaccacatg
ctcggcgacg ctgatcgagt 1740 tgcagtaggt gacttccgcc acggtgacgt
tgcggtcgat agccgactga agatcgacgt 1800 ggtcggaacc gatgccggcg
gtgagcgcga gcttcaggtt cttggccttg gcgatgcgct 1860 cgggcgtcag
ataggccggc cagaagggct gggagatgac gacatccgca tcgaccagct 1920
cgcgctcgaa caccgagtcg gggccgtcct tgtcggaggt cacgaccagg gtgtggccgt
1980 tggattcgag atattcgcgc aggccgagct cgccggagac ggagccgagc
aactgcccgg 2040 gcgtgaagtc gatggccttc ggcgtcggca agatctggcc
gcccggatag tggtcgatct 2100 tcggaagatc gtcgcgggca taggtcttcg
ggtagccgtc gaccggatca tcgtaaagaa 2160 cgcacaggac ctttgccatc
atatgtatat ctccttctta aagttaaaca aaattatttc 2220 tagaggggaa
ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg 2280
atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
2340 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
tcgccacttc 2400 gggctcatga gcgcttgttt cggcgtgggt atggtggcag
gccccgtggc cgggggactg 2460 ttgggcgcca tctccttgca tgcaccattc
cttgcggcgg cggtgctcaa cggcctcaac 2520 ctactactgg gctgcttcct
aatgcaggag tcgcataagg gagagcgtcg agatcccgga 2580 caccatcgaa
tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca 2640
attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
2700 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
cgaaaacgcg 2760 ggaaaaagtg gaagcggcga tggcggagct gaattacatt
cccaaccgcg tggcacaaca 2820 actggcgggc aaacagtcgt tgctgattgg
cgttgccacc tccagtctgg ccctgcacgc 2880 gccgtcgcaa attgtcgcgg
cgattaaatc tcgcgccgat caactgggtg ccagcgtggt 2940 ggtgtcgatg
gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct 3000
cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
3060 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
accagacacc 3120 catcaacagt attattttct cccatgaaga cggtacgcga
ctgggcgtgg agcatctggt 3180 cgcattgggt caccagcaaa tcgcgctgtt
agcgggccca ttaagttctg tctcggcgcg 3240 tctgcgtctg gctggctggc
ataaatatct cactcgcaat caaattcagc cgatagcgga 3300 acgggaaggc
gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga 3360
gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
3420 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
gatacgacga 3480 taccgaagac agctcatgtt atatcccgcc gttaaccacc
atcaaacagg attttcgcct 3540 gctggggcaa accagcgtgg accgcttgct
gcaactctct cagggccagg cggtgaaggg 3600 caatcagctg ttgcccgtct
cactggtgaa aagaaaaacc accctggcgc ccaatacgca 3660 aaccgcctct
ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 3720
actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac
3780 cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc
cggtgggcgc 3840 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
tatcatgcaa ctcgtaggac 3900 aggtgccggc agcgctctgg gtcattttcg
gcgaggaccg ctttcgctgg agcgcgacga 3960 tgatcggcct gtcgcttgcg
gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca 4020 ctggtcccgc
caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc 4080
cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
4140 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
tgctgctgca 4200 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
tttccgtgtt tcgtaaagtc 4260 tggaaacgcg gaagtcagcg ccctgcacca
ttatgttccg gatctgcatc gcaggatgct 4320 gctggctacc ctgtggaaca
cctacatctg tattaacgaa gcgctggcat tgaccctgag 4380 tgatttttct
ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 4440
gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
4500 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
accaaacagg 4560 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
gacattaacg cttctggaga 4620 aactcaacga gctggacgcg gatgaacagg
cagacatctg tgaatcgctt cacgaccacg 4680 ctgatgagct ttaccgcagc
tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 4740 acatgcagct
cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 4800
cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
4860 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
ttgtactgag 4920 agtgcaccat atatgcggtg tgaaataccg cacagatgcg
taaggagaaa ataccgcatc 4980 aggcgctctt ccgcttcctc gctcactgac
tcgctgcgct cggtcgttcg gctgcggcga 5040 gcggtatcag ctcactcaaa
ggcggtaata cggttatcca cagaatcagg ggataacgca 5100 ggaaagaaca
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 5160
ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5220 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
tggaagctcc 5280 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
acctgtccgc ctttctccct 5340 tcgggaagcg tggcgctttc tcatagctca
cgctgtaggt atctcagttc ggtgtaggtc 5400 gttcgctcca agctgggctg
tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 5460 tccggtaact
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 5520
gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5580 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
tctgctgaag 5640 ccagttacct tcggaaaaag agttggtagc tcttgatccg
gcaaacaaac caccgctggt 5700 agcggtggtt tttttgtttg caagcagcag
attacgcgca gaaaaaaagg atctcaagaa 5760 gatcctttga tcttttctac
ggggtctgac gctcagtgga acgaaaactc acgttaaggg 5820 attttggtca
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 5880
agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5940 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
tgcctgactc 6000 cccgtcgtgt agataactac gatacgggag ggcttaccat
ctggccccag tgctgcaatg 6060 ataccgcgag acccacgctc accggctcca
gatttatcag caataaacca gccagccgga 6120 agggccgagc gcagaagtgg
tcctgcaact ttatccgcct ccatccagtc tattaattgt 6180 tgccgggaag
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 6240
gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6300 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
tagctccttc 6360 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
tatcactcat ggttatggca 6420 gcactgcata attctcttac tgtcatgcca
tccgtaagat gcttttctgt gactggtgag 6480 tactcaacca agtcattctg
agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 6540 tcaatacggg
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 6600
cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6660 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
ttctgggtga 6720 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
gggcgacacg gaaatgttga 6780 atactcatac tcttcctttt tcaatattat
tgaagcattt atcagggtta ttgtctcatg 6840 agcggataca tatttgaatg
tatttagaaa aataaacaaa taggggttcc gcgcacattt 6900 ccccgaaaag
tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 6960
ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
7020 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
gagtccacta 7080 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
tctatcaggg cgatggccca 7140 ctacgtgaac catcacccta atcaagtttt
ttggggtcga ggtgccgtaa agcactaaat 7200 cggaacccta aagggagccc
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 7260 agaaaggaag
ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 7320
acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
7380 tcgcca 7386
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 69 <210>
SEQ ID NO 1 <211> LENGTH: 792 <212> TYPE: DNA
<213> ORGANISM: Collinsella aerofaciens <220> FEATURE:
<221> NAME/KEY: CDS <222> LOCATION: (1)..(792)
<400> SEQUENCE: 1 atg aac ctg agg gag aag tac ggt gag tgg ggc
ctg atc ctg ggc gcg 48 Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly
Leu Ile Leu Gly Ala 1 5 10 15 acc gag ggc gtc ggc aag gcg ttc tgc
gag aag atc gcc gcc ggc ggc 96 Thr Glu Gly Val Gly Lys Ala Phe Cys
Glu Lys Ile Ala Ala Gly Gly 20 25 30 atg aac gtc gtc atg gtc ggc
cgt cgc gag gag aag ctg aac gtg ctc 144 Met Asn Val Val Met Val Gly
Arg Arg Glu Glu Lys Leu Asn Val Leu 35 40 45 gca ggc gag atc cgc
gag acc tac ggc gtg gag acc aag gtc gtg cgc 192 Ala Gly Glu Ile Arg
Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60 gcc gac ttt
agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc 240 Ala Asp Phe
Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65 70 75 80 gag
ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc 288 Glu
Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 85 90
95 ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc
336 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile
100 105 110 aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac
atg cgg 384 Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr
Met Arg 115 120 125 atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac
gtc tcg tcg atg 432 Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn
Val Ser Ser Met 130 135 140 acc ggc atc agc tcc agc ccc tgg aac ggc
cag tac ggc gcg ggc aag 480 Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly
Gln Tyr Gly Ala Gly Lys 145 150 155 160 gcc ttc atc ctc aag atg acc
gag gcc gtg gcc tgc gag tgc gag ggc 528 Ala Phe Ile Leu Lys Met Thr
Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175 acc ggc gtc gac gtc
gag gtc atc acc ctc ggc acc acc cta acc ccc 576 Thr Gly Val Asp Val
Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185 190 agc ctg ctg
tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg 624 Ser Leu Leu
Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 195 200 205 aag
atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag 672 Lys
Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210 215
220 ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc
720 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser
225 230 235 240 gtc cac gac tgg aag gca aac cac acc gag gac gag tac
atc cgc tac 768 Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr
Ile Arg Tyr 245 250 255 atg ggg tcg ttc tac cgc gac tag 792 Met Gly
Ser Phe Tyr Arg Asp 260 <210> SEQ ID NO 2 <211> LENGTH:
263 <212> TYPE: PRT <213> ORGANISM: Collinsella
aerofaciens <400> SEQUENCE: 2 Met Asn Leu Arg Glu Lys Tyr Gly
Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu Gly Val Gly Lys
Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30 Met Asn Val Val
Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu 35 40 45 Ala Gly
Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50 55 60
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 65
70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu
His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His
Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val Thr Phe Leu Lys Cys
Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln Asp Arg Gly
Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr Gly Ile Ser Ser Ser
Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160 Ala Phe Ile
Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175 Thr
Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180 185
190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met
195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe
Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg
Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp Lys Ala Asn His Thr
Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe Tyr Arg Asp
260 <210> SEQ ID NO 3 <211> LENGTH: 31 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 3
gggaattcca tatgaacctg agggagaagt a 31 <210> SEQ ID NO 4
<211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 4 cccaagcttc tagtcgcggt agaacga 27
<210> SEQ ID NO 5 <211> LENGTH: 774 <212> TYPE:
DNA <213> ORGANISM: Comamonas testosteroni <220>
FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(774)
<400> SEQUENCE: 5 atg tcc atc atc gtg ata agc ggc tgc gcc acc
ggc att ggt gcc gct 48 Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr
Gly Ile Gly Ala Ala 1 5 10 15 acg cgc aag gtc ctg gag gcg gcc ggt
cac cag atc gta ggc atc gat 96 Thr Arg Lys Val Leu Glu Ala Ala Gly
His Gln Ile Val Gly Ile Asp 20 25 30 ata cgc gat gcg gaa gtg att
gcc gat ctc tcg acg gcc gaa ggt cga 144 Ile Arg Asp Ala Glu Val Ile
Ala Asp Leu Ser Thr Ala Glu Gly Arg 35 40 45 aag cag gcg att gcc
gat gta ctg gcg aag tgc agc aag ggc atg gac 192 Lys Gln Ala Ile Ala
Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp 50 55 60 ggc ctg gtg
ctg tgc gcc ggc ctg gga ccg cag acc aag gtg ctt ggc 240 Gly Leu Val
Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly 65 70 75 80 aat
gtg gtt tcg gtc aat tat ttt ggc gcg acc gag ctg atg gat gcc 288 Asn
Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala 85 90
95 ttt ttg cca gcg ctg aaa aaa ggc cat cag ccc gca gcc gtc gtc atc
336 Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile
100 105 110 tcg tcc gtg gct tcc gcg cat ctg gct ttt gac aag aac cca
ctg gcg 384 Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro
Leu Ala 115 120 125 ctg gca ctg gaa gcc ggc gag gaa gcc aag gcc cgc
gcc att gtc gaa 432 Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg
Ala Ile Val Glu 130 135 140 cat gcg gga gag cag ggc gga aat ctg gcc
tat gcg ggc agc aag aat 480 His Ala Gly Glu Gln Gly Gly Asn Leu Ala
Tyr Ala Gly Ser Lys Asn 145 150 155 160 gct ttg acg gtg gct gtg cgc
aaa cgc gcc gcc gcc tgg ggc gag gct 528 Ala Leu Thr Val Ala Val Arg
Lys Arg Ala Ala Ala Trp Gly Glu Ala 165 170 175 ggc gtg cgc ctg aac
acc atc gcc ccc ggt gca acc gag act ccc ttg 576 Gly Val Arg Leu Asn
Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu 180 185 190 ctg cag gcg
ggc ctg cag gac ccg cgc tat ggc gaa tcc att gcc aag 624 Leu Gln Ala
Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys 195 200 205 ttc
gtt cct ccc atg ggc cgc cgt gcc gag ccg tcc gag atg gcg tcg 672 Phe
Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser 210 215
220 gtc atc gcc ttt ttg atg agc ccg gcc gca agc tat gtg cat ggc gcg
720 Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala
225 230 235 240 cag atc gtc att gat ggc ggc att gat gcg gtg atg cgc
ccg aca cag 768 Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg
Pro Thr Gln 245 250 255 ttc tga 774 Phe <210> SEQ ID NO 6
<211> LENGTH: 257 <212> TYPE: PRT <213> ORGANISM:
Comamonas testosteroni
<400> SEQUENCE: 6 Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr
Gly Ile Gly Ala Ala 1 5 10 15 Thr Arg Lys Val Leu Glu Ala Ala Gly
His Gln Ile Val Gly Ile Asp 20 25 30 Ile Arg Asp Ala Glu Val Ile
Ala Asp Leu Ser Thr Ala Glu Gly Arg 35 40 45 Lys Gln Ala Ile Ala
Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp 50 55 60 Gly Leu Val
Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly 65 70 75 80 Asn
Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala 85 90
95 Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile
100 105 110 Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro
Leu Ala 115 120 125 Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg
Ala Ile Val Glu 130 135 140 His Ala Gly Glu Gln Gly Gly Asn Leu Ala
Tyr Ala Gly Ser Lys Asn 145 150 155 160 Ala Leu Thr Val Ala Val Arg
Lys Arg Ala Ala Ala Trp Gly Glu Ala 165 170 175 Gly Val Arg Leu Asn
Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu 180 185 190 Leu Gln Ala
Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys 195 200 205 Phe
Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser 210 215
220 Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala
225 230 235 240 Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg
Pro Thr Gln 245 250 255 Phe <210> SEQ ID NO 7 <211>
LENGTH: 969 <212> TYPE: DNA <213> ORGANISM: Rattus
norvegicus <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(969) <400> SEQUENCE: 7 atg gat
tcc ata tct ctg cgt gta gca cta aat gat ggt aac ttc att 48 Met Asp
Ser Ile Ser Leu Arg Val Ala Leu Asn Asp Gly Asn Phe Ile 1 5 10 15
cct gta ctg ggg ttt gga acc act gtg cct gag aag gtt gct aag gat 96
Pro Val Leu Gly Phe Gly Thr Thr Val Pro Glu Lys Val Ala Lys Asp 20
25 30 gaa gtt atc aag gct act aaa ata gct ata gat aat gga ttc cgc
cat 144 Glu Val Ile Lys Ala Thr Lys Ile Ala Ile Asp Asn Gly Phe Arg
His 35 40 45 ttt gac tct gct tat ttg tac gaa gta gaa gag gaa gtg
ggc caa gcc 192 Phe Asp Ser Ala Tyr Leu Tyr Glu Val Glu Glu Glu Val
Gly Gln Ala 50 55 60 att aga agc aag att gaa gac ggc act gtg aag
aga gaa gat ata ttc 240 Ile Arg Ser Lys Ile Glu Asp Gly Thr Val Lys
Arg Glu Asp Ile Phe 65 70 75 80 tat act tca aag ctt tgg agc act ttc
cat aga cca gag ctg gtc cga 288 Tyr Thr Ser Lys Leu Trp Ser Thr Phe
His Arg Pro Glu Leu Val Arg 85 90 95 act tgc ttg gaa aag aca ctg
aaa agc act caa ctg gac tat gtg gat 336 Thr Cys Leu Glu Lys Thr Leu
Lys Ser Thr Gln Leu Asp Tyr Val Asp 100 105 110 ctt tat att att cat
ttc cca atg gct ttg cag cct gga gat ata ttt 384 Leu Tyr Ile Ile His
Phe Pro Met Ala Leu Gln Pro Gly Asp Ile Phe 115 120 125 ttc cca cga
gat gag cat gga aaa cta ttg ttt gaa aca gtg gat atc 432 Phe Pro Arg
Asp Glu His Gly Lys Leu Leu Phe Glu Thr Val Asp Ile 130 135 140 tgt
gac aca tgg gag gcc atg gaa aag tgt aag gat gca gga ttg gcc 480 Cys
Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp Ala Gly Leu Ala 145 150
155 160 aag tct att ggg gtg tcc aac ttt aac tgc agg cag ctg gag agg
att 528 Lys Ser Ile Gly Val Ser Asn Phe Asn Cys Arg Gln Leu Glu Arg
Ile 165 170 175 ctg aat aag cca ggg ctc aaa tac aag cct gtg tgc aac
cag gtg gaa 576 Leu Asn Lys Pro Gly Leu Lys Tyr Lys Pro Val Cys Asn
Gln Val Glu 180 185 190 tgt cac ctt tat ctc aac cag agc aaa atg ctg
gac tat tgt aag tca 624 Cys His Leu Tyr Leu Asn Gln Ser Lys Met Leu
Asp Tyr Cys Lys Ser 195 200 205 aaa gac atc att ctg gtt tcc tac tgc
acg ctg gga agt tca cga gac 672 Lys Asp Ile Ile Leu Val Ser Tyr Cys
Thr Leu Gly Ser Ser Arg Asp 210 215 220 aaa aca tgg gtg gat cag aaa
agt cca gtt ctc cta gat gat cca gtt 720 Lys Thr Trp Val Asp Gln Lys
Ser Pro Val Leu Leu Asp Asp Pro Val 225 230 235 240 ctt tgt gcc ata
gca aag aag tac aag caa acc cca gcc cta gtt gcc 768 Leu Cys Ala Ile
Ala Lys Lys Tyr Lys Gln Thr Pro Ala Leu Val Ala 245 250 255 ctt cgc
tac cag ctg cag cgt ggg gtt gtg ccc ctg atc agg agt ttc 816 Leu Arg
Tyr Gln Leu Gln Arg Gly Val Val Pro Leu Ile Arg Ser Phe 260 265 270
aac gcg aag cgg atc aaa gag cta aca cag gtt ttt gaa ttc cag ttg 864
Asn Ala Lys Arg Ile Lys Glu Leu Thr Gln Val Phe Glu Phe Gln Leu 275
280 285 gct tca gag gac atg aaa gcc ctg gat ggc ttg aac aga aat ttc
aga 912 Ala Ser Glu Asp Met Lys Ala Leu Asp Gly Leu Asn Arg Asn Phe
Arg 290 295 300 tac aac aat gca aaa tat ttt gat gac cat ccc aat cat
cca ttt act 960 Tyr Asn Asn Ala Lys Tyr Phe Asp Asp His Pro Asn His
Pro Phe Thr 305 310 315 320 gat gaa tag 969 Asp Glu <210> SEQ
ID NO 8 <211> LENGTH: 322 <212> TYPE: PRT <213>
ORGANISM: Rattus norvegicus <400> SEQUENCE: 8 Met Asp Ser Ile
Ser Leu Arg Val Ala Leu Asn Asp Gly Asn Phe Ile 1 5 10 15 Pro Val
Leu Gly Phe Gly Thr Thr Val Pro Glu Lys Val Ala Lys Asp 20 25 30
Glu Val Ile Lys Ala Thr Lys Ile Ala Ile Asp Asn Gly Phe Arg His 35
40 45 Phe Asp Ser Ala Tyr Leu Tyr Glu Val Glu Glu Glu Val Gly Gln
Ala 50 55 60 Ile Arg Ser Lys Ile Glu Asp Gly Thr Val Lys Arg Glu
Asp Ile Phe 65 70 75 80 Tyr Thr Ser Lys Leu Trp Ser Thr Phe His Arg
Pro Glu Leu Val Arg 85 90 95 Thr Cys Leu Glu Lys Thr Leu Lys Ser
Thr Gln Leu Asp Tyr Val Asp 100 105 110 Leu Tyr Ile Ile His Phe Pro
Met Ala Leu Gln Pro Gly Asp Ile Phe 115 120 125 Phe Pro Arg Asp Glu
His Gly Lys Leu Leu Phe Glu Thr Val Asp Ile 130 135 140 Cys Asp Thr
Trp Glu Ala Met Glu Lys Cys Lys Asp Ala Gly Leu Ala 145 150 155 160
Lys Ser Ile Gly Val Ser Asn Phe Asn Cys Arg Gln Leu Glu Arg Ile 165
170 175 Leu Asn Lys Pro Gly Leu Lys Tyr Lys Pro Val Cys Asn Gln Val
Glu 180 185 190 Cys His Leu Tyr Leu Asn Gln Ser Lys Met Leu Asp Tyr
Cys Lys Ser 195 200 205 Lys Asp Ile Ile Leu Val Ser Tyr Cys Thr Leu
Gly Ser Ser Arg Asp 210 215 220 Lys Thr Trp Val Asp Gln Lys Ser Pro
Val Leu Leu Asp Asp Pro Val 225 230 235 240 Leu Cys Ala Ile Ala Lys
Lys Tyr Lys Gln Thr Pro Ala Leu Val Ala 245 250 255 Leu Arg Tyr Gln
Leu Gln Arg Gly Val Val Pro Leu Ile Arg Ser Phe 260 265 270 Asn Ala
Lys Arg Ile Lys Glu Leu Thr Gln Val Phe Glu Phe Gln Leu 275 280 285
Ala Ser Glu Asp Met Lys Ala Leu Asp Gly Leu Asn Arg Asn Phe Arg 290
295 300 Tyr Asn Asn Ala Lys Tyr Phe Asp Asp His Pro Asn His Pro Phe
Thr 305 310 315 320 Asp Glu <210> SEQ ID NO 9 <211>
LENGTH: 26 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 9 cgtcgtcatg gtcgcccgtc gcgagg 26 <210>
SEQ ID NO 10 <211> LENGTH: 26 <212> TYPE: DNA
<213> ORGANISM: Artificial <220> FEATURE: <223>
OTHER INFORMATION: PCR Primer <400> SEQUENCE: 10 cctcgcgacg
ggcgaccatg acgacg 26 <210> SEQ ID NO 11 <211> LENGTH:
26 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 11 cgtcgtcatg gtcagccgtc gcgagg 26
<210> SEQ ID NO 12 <211> LENGTH: 26 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 12
cctcgcgacg gctgaccatg acgacg 26 <210> SEQ ID NO 13
<211> LENGTH: 47 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 13 gcgaattcgt gaaaggagat atacatgaac
ctgagggaga agtacgg 47 <210> SEQ ID NO 14 <211> LENGTH:
1206 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: Formate
Dehydrogenase Mutant <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(1206) <400> SEQUENCE: 14 atg gca
aag gtc ctg tgc gtt ctt tac gat gat ccg gtc gac ggc tac 48 Met Ala
Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 1 5 10 15
ccg aag acc tat gcc cgc gac gat ctt ccg aag atc gac cac tat ccg 96
Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 20
25 30 ggc ggc cag atc ttg ccg acg ccg aag gcc atc gac ttc acg ccc
ggg 144 Gly Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro
Gly 35 40 45 cag ttg ctc ggc tcc gtc tcc ggc gag ctc ggc ctg cgc
gaa tat ctc 192 Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg
Glu Tyr Leu 50 55 60 gaa tcc aac ggc cac acc ctg gtc gtg acc tcc
gac aag gac ggc ccc 240 Glu Ser Asn Gly His Thr Leu Val Val Thr Ser
Asp Lys Asp Gly Pro 65 70 75 80 gac tcg gtg ttc gag cgc gag ctg gtc
gat gcg gat gtc gtc atc tcc 288 Asp Ser Val Phe Glu Arg Glu Leu Val
Asp Ala Asp Val Val Ile Ser 85 90 95 cag ccc ttc tgg ccg gcc tat
ctg acg ccc gag cgc atc gcc aag gcc 336 Gln Pro Phe Trp Pro Ala Tyr
Leu Thr Pro Glu Arg Ile Ala Lys Ala 100 105 110 aag aac ctg aag ctc
gcg ctc acc gcc ggc atc ggt tcc gac cac gtc 384 Lys Asn Leu Lys Leu
Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val 115 120 125 gat ctt cag
tcg gct atc gac cgc aac gtc acc gtg gcg gaa gtc acc 432 Asp Leu Gln
Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr 130 135 140 tac
tgc aac tcg atc agc gtc gcc gag cat gtg gtg atg atg atc ctg 480 Tyr
Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 145 150
155 160 tcg ctg gtg cgc aac tat ctg ccc tcg cac gaa tgg gcg cgg aag
ggc 528 Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys
Gly 165 170 175 ggc tgg aac atc gcc gac tgc gtc tcc cac gcc tac gac
ctc gag gcg 576 Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp
Leu Glu Ala 180 185 190 atg cat gtc ggc acc gtg gcc gcc ggc cgc atc
ggt ctc gcg gtg ctg 624 Met His Val Gly Thr Val Ala Ala Gly Arg Ile
Gly Leu Ala Val Leu 195 200 205 cgc cgt ctg gcg ccg ttc gac gtg cac
ctg cac tac acc ggc cgt cac 672 Arg Arg Leu Ala Pro Phe Asp Val His
Leu His Tyr Thr Gly Arg His 210 215 220 cgc ctg ccg gaa tcg gtc gag
aag gag ctc aac ctc acc tgg cac gcg 720 Arg Leu Pro Glu Ser Val Glu
Lys Glu Leu Asn Leu Thr Trp His Ala 225 230 235 240 acc cgc gag gac
atg tat ccg gtt tgc gac gtg gtg acg ctg aac tgc 768 Thr Arg Glu Asp
Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 245 250 255 ccg ctg
cac ccc gaa acc gag cac atg atc aat gac gag acg ctg aag 816 Pro Leu
His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 260 265 270
ctg ttc aag cgt ggc gcc tac atc gtc aac acc gcc cgc ggc aag ctg 864
Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 275
280 285 tgc gac cgc gat gcc gtg gca cgt gcg ctc gaa tcc ggc cgg ctg
gcc 912 Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu
Ala 290 295 300 ggc tat gcc ggc gac gtg tgg ttc ccg cag ccg gcg ccg
aag gac cac 960 Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro
Lys Asp His 305 310 315 320 ccc tgg cgg acg atg ccc tat aac ggc atg
acc ccg cac atc tcc ggc 1008 Pro Trp Arg Thr Met Pro Tyr Asn Gly
Met Thr Pro His Ile Ser Gly 325 330 335 acc acg ctg acc gcg cag gcg
cgt tat gcg gcg ggc acc cgc gag atc 1056 Thr Thr Leu Thr Ala Gln
Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 340 345 350 ctg gag tgc ttc
ttc gag ggc cgt ccg atc cgc gac gaa tac ctc atc 1104 Leu Glu Cys
Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355 360 365 gtg
cag ggc ggc gct ctt gcc ggc acc ggc gcg cat tcc tac tcg aag 1152
Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 370
375 380 ggc aat gcc acc ggc ggt tcg gaa gag gcc gcc aag ttc aag aag
gcg 1200 Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys
Lys Ala 385 390 395 400 gtc tga 1206 Val <210> SEQ ID NO 15
<211> LENGTH: 401 <212> TYPE: PRT <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION:
Synthetic Construct <400> SEQUENCE: 15 Met Ala Lys Val Leu
Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 1 5 10 15 Pro Lys Thr
Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 20 25 30 Gly
Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 35 40
45 Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu
50 55 60 Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp
Gly Pro 65 70 75 80 Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp
Val Val Ile Ser 85 90 95 Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro
Glu Arg Ile Ala Lys Ala 100 105 110 Lys Asn Leu Lys Leu Ala Leu Thr
Ala Gly Ile Gly Ser Asp His Val 115 120 125 Asp Leu Gln Ser Ala Ile
Asp Arg Asn Val Thr Val Ala Glu Val Thr 130 135 140 Tyr Cys Asn Ser
Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 145 150 155 160 Ser
Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 165 170
175 Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala
180 185 190 Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala
Val Leu 195 200 205 Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr
Thr Gly Arg His 210 215 220 Arg Leu Pro Glu Ser Val Glu Lys Glu Leu
Asn Leu Thr Trp His Ala 225 230 235 240 Thr Arg Glu Asp Met Tyr Pro
Val Cys Asp Val Val Thr Leu Asn Cys 245 250 255 Pro Leu His Pro Glu
Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 260 265 270 Leu Phe Lys
Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 275 280 285 Cys
Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 290 295
300 Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His
305 310 315 320 Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His
Ile Ser Gly 325 330 335 Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala
Gly Thr Arg Glu Ile 340 345 350 Leu Glu Cys Phe Phe Glu Gly Arg Pro
Ile Arg Asp Glu Tyr Leu Ile 355 360 365 Val Gln Gly Gly Ala Leu Ala
Gly Thr Gly Ala His Ser Tyr Ser Lys 370 375 380 Gly Asn Ala Thr Gly
Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 385 390 395 400 Val
<210> SEQ ID NO 16 <211> LENGTH: 43 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 16
cccaagctta aggagatata catgtccatc atcgtgataa gcg 43 <210> SEQ
ID NO 17 <211> LENGTH: 34 <212> TYPE: DNA <213>
ORGANISM: Artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer <400> SEQUENCE: 17 ataagaatgc
ggccgctcag aactgtgtcg ggcg 34
<210> SEQ ID NO 18 <211> LENGTH: 7404 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pET21a FDH D221G 7beta-HSDH
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (5208)..(6422) <223> OTHER INFORMATION: FDH D221G
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (6435)..(7226) <223> OTHER INFORMATION: 7beta-HSDH
<400> SEQUENCE: 18 tggcgaatgg gacgcgccct gtagcggcgc
attaagcgcg gcgggtgtgg tggttacgcg 60 cagcgtgacc gctacacttg
ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120 ctttctcgcc
acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
240 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
agtccacgtt 300 ctttaatagt ggactcttgt tccaaactgg aacaacactc
aaccctatct cggtctattc 360 ttttgattta taagggattt tgccgatttc
ggcctattgg ttaaaaaatg agctgattta 420 acaaaaattt aacgcgaatt
ttaacaaaat attaacgttt acaatttcag gtggcacttt 480 tcggggaaat
gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
600 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
gccttcctgt 660 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
gaagatcagt tgggtgcacg 720 agtgggttac atcgaactgg atctcaacag
cggtaagatc cttgagagtt ttcgccccga 780 agaacgtttt ccaatgatga
gcacttttaa agttctgcta tgtggcgcgg tattatcccg 840 tattgacgcc
gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 900
tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
960 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
caacgatcgg 1020 aggaccgaag gagctaaccg cttttttgca caacatgggg
gatcatgtaa ctcgccttga 1080 tcgttgggaa ccggagctga atgaagccat
accaaacgac gagcgtgaca ccacgatgcc 1140 tgcagcaatg gcaacaacgt
tgcgcaaact attaactggc gaactactta ctctagcttc 1200 ccggcaacaa
ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1260
ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
1320 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
ttatctacac 1380 gacggggagt caggcaacta tggatgaacg aaatagacag
atcgctgaga taggtgcctc 1440 actgattaag cattggtaac tgtcagacca
agtttactca tatatacttt agattgattt 1500 aaaacttcat ttttaattta
aaaggatcta ggtgaagatc ctttttgata atctcatgac 1560 caaaatccct
taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1620
aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
1680 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
ttccgaaggt 1740 aactggcttc agcagagcgc agataccaaa tactgtcctt
ctagtgtagc cgtagttagg 1800 ccaccacttc aagaactctg tagcaccgcc
tacatacctc gctctgctaa tcctgttacc 1860 agtggctgct gccagtggcg
ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1920 accggataag
gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1980
gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
2040 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
caggagagcg 2100 cacgagggag cttccagggg gaaacgcctg gtatctttat
agtcctgtcg ggtttcgcca 2160 cctctgactt gagcgtcgat ttttgtgatg
ctcgtcaggg gggcggagcc tatggaaaaa 2220 cgccagcaac gcggcctttt
tacggttcct ggccttttgc tggccttttg ctcacatgtt 2280 ctttcctgcg
ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2340
taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
2400 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
gcatatatgg 2460 tgcactctca gtacaatctg ctctgatgcc gcatagttaa
gccagtatac actccgctat 2520 cgctacgtga ctgggtcatg gctgcgcccc
gacacccgcc aacacccgct gacgcgccct 2580 gacgggcttg tctgctcccg
gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2640 gcatgtgtca
gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2700
catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
2760 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
ttaagggcgg 2820 ttttttcctg tttggtcact gatgcctccg tgtaaggggg
atttctgttc atgggggtaa 2880 tgataccgat gaaacgagag aggatgctca
cgatacgggt tactgatgat gaacatgccc 2940 ggttactgga acgttgtgag
ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3000 aaatcactca
gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3060
gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
3120 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
caggtcgcag 3180 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
cggtgattca ttctgctaac 3240 cagtaaggca accccgccag cctagccggg
tcctcaacga caggagcacg atcatgcgca 3300 cccgtggggc cgccatgccg
gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3360 gaccagtgac
gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3420
cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
3480 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
acgatagtca 3540 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
tctcaagggc atcggtcgag 3600 atcccggtgc ctaatgagtg agctaactta
cattaattgc gttgcgctca ctgcccgctt 3660 tccagtcggg aaacctgtcg
tgccagctgc attaatgaat cggccaacgc gcggggagag 3720 gcggtttgcg
tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3780
tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
3840 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
tgagctgtct 3900 tcggtatcgt cgtatcccac taccgagata tccgcaccaa
cgcgcagccc ggactcggta 3960 atggcgcgca ttgcgcccag cgccatctga
tcgttggcaa ccagcatcgc agtgggaacg 4020 atgccctcat tcagcatttg
catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4080 tcccgttccg
ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4140
cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
4200 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
aataatactg 4260 ttgatgggtg tctggtcaga gacatcaaga aataacgccg
gaacattagt gcaggcagct 4320 tccacagcaa tggcatcctg gtcatccagc
ggatagttaa tgatcagccc actgacgcgt 4380 tgcgcgagaa gattgtgcac
cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4440 gacaccacca
cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4500
gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
4560 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
cgcttccact 4620 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
ccacgcggga aacggtctga 4680 taagagacac cggcatactc tgcgacatcg
tataacgtta ctggtttcac attcaccacc 4740 ctgaattgac tctcttccgg
gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4800 atggtgtccg
ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4860
tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
4920 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
aagcgctcat 4980 gagcccgaag tggcgagccc gatcttcccc atcggtgatg
tcggcgatat aggcgccagc 5040 aaccgcacct gtggcgccgg tgatgccggc
cacgatgcgt ccggcgtaga ggatcgagat 5100 ctcgatcccg cgaaattaat
acgactcact ataggggaat tgtgagcgga taacaattcc 5160 cctctagaaa
taattttgtt taactttaag aaggagatat acatatg atg gca aag 5216 Met Ala
Lys 1 gtc ctg tgc gtt ctt tac gat gat ccg gtc gac ggc tac ccg aag
acc 5264 Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr Pro
Lys Thr 5 10 15 tat gcc cgc gac gat ctt ccg aag atc gac cac tat ccg
ggc ggc cag 5312 Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr
Pro Gly Gly Gln 20 25 30 35 atc ttg ccg acg ccg aag gcc atc gac ttc
acg ccc ggg cag ttg ctc 5360 Ile Leu Pro Thr Pro Lys Ala Ile Asp
Phe Thr Pro Gly Gln Leu Leu 40 45 50 ggc tcc gtc tcc ggc gag ctc
ggc ctg cgc gaa tat ctc gaa tcc aac 5408 Gly Ser Val Ser Gly Glu
Leu Gly Leu Arg Glu Tyr Leu Glu Ser Asn 55 60 65 ggc cac acc ctg
gtc gtg acc tcc gac aag gac ggc ccc gac tcg gtg 5456 Gly His Thr
Leu Val Val Thr Ser Asp Lys Asp Gly Pro Asp Ser Val 70 75 80 ttc
gag cgc gag ctg gtc gat gcg gat gtc gtc atc tcc cag ccc ttc 5504
Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser Gln Pro Phe 85
90 95 tgg ccg gcc tat ctg acg ccc gag cgc atc gcc aag gcc aag aac
ctg 5552 Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala Lys
Asn Leu 100 105 110 115 aag ctc gcg ctc acc gcc ggc atc ggt tcc gac
cac gtc gat ctt cag 5600 Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser
Asp His Val Asp Leu Gln 120 125 130 tcg gct atc gac cgc aac gtc acc
gtg gcg gaa gtc acc tac tgc aac 5648 Ser Ala Ile Asp Arg Asn Val
Thr Val Ala Glu Val Thr Tyr Cys Asn 135 140 145 tcg atc agc gtc gcc
gag cat gtg gtg atg atg atc ctg tcg ctg gtg 5696 Ser Ile Ser Val
Ala Glu His Val Val Met Met Ile Leu Ser Leu Val 150 155 160 cgc aac
tat ctg ccc tcg cac gaa tgg gcg cgg aag ggc ggc tgg aac 5744 Arg
Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly Gly Trp Asn 165 170
175 atc gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg atg cat gtc
5792 Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala Met His
Val 180 185 190 195 ggc acc gtg gcc gcc ggc cgc atc ggt ctc gcg gtg
ctg cgc cgt ctg 5840 Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala
Val Leu Arg Arg Leu 200 205 210 gcg ccg ttc gac gtg cac ctg cac tac
acc ggc cgt cac cgc ctg ccg 5888 Ala Pro Phe Asp Val His Leu His
Tyr Thr Gly Arg His Arg Leu Pro 215 220 225 gaa tcg gtc gag aag gag
ctc aac ctc acc tgg cac gcg acc cgc gag 5936 Glu Ser Val Glu Lys
Glu Leu Asn Leu Thr Trp His Ala Thr Arg Glu
230 235 240 gac atg tat ccg gtt tgc gac gtg gtg acg ctg aac tgc ccg
ctg cac 5984 Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys
Pro Leu His 245 250 255 ccc gaa acc gag cac atg atc aat gac gag acg
ctg aag ctg ttc aag 6032 Pro Glu Thr Glu His Met Ile Asn Asp Glu
Thr Leu Lys Leu Phe Lys 260 265 270 275 cgt ggc gcc tac atc gtc aac
acc gcc cgc ggc aag ctg tgc gac cgc 6080 Arg Gly Ala Tyr Ile Val
Asn Thr Ala Arg Gly Lys Leu Cys Asp Arg 280 285 290 gat gcc gtg gca
cgt gcg ctc gaa tcc ggc cgg ctg gcc ggc tat gcc 6128 Asp Ala Val
Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala Gly Tyr Ala 295 300 305 ggc
gac gtg tgg ttc ccg cag ccg gcg ccg aag gac cac ccc tgg cgg 6176
Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His Pro Trp Arg 310
315 320 acg atg ccc tat aac ggc atg acc ccg cac atc tcc ggc acc acg
ctg 6224 Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly Thr
Thr Leu 325 330 335 acc gcg cag gcg cgt tat gcg gcg ggc acc cgc gag
atc ctg gag tgc 6272 Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg
Glu Ile Leu Glu Cys 340 345 350 355 ttc ttc gag ggc cgt ccg atc cgc
gac gaa tac ctc atc gtg cag ggc 6320 Phe Phe Glu Gly Arg Pro Ile
Arg Asp Glu Tyr Leu Ile Val Gln Gly 360 365 370 ggc gct ctt gcc ggc
acc ggc gcg cat tcc tac tcg aag ggc aat gcc 6368 Gly Ala Leu Ala
Gly Thr Gly Ala His Ser Tyr Ser Lys Gly Asn Ala 375 380 385 acc ggc
ggt tcg gaa gag gcc gcc aag ttc aag aag gcg gct gag aat 6416 Thr
Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala Ala Glu Asn 390 395
400 tcg tga aaggagatat ac atg aac ctg agg gag aag tac ggt gag tgg
ggc 6467 Ser Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly 405 410
415 ctg atc ctg ggc gcg acc gag ggc gtc ggc aag gcg ttc tgc gag aag
6515 Leu Ile Leu Gly Ala Thr Glu Gly Val Gly Lys Ala Phe Cys Glu
Lys 420 425 430 atc gcc gcc ggc ggc atg aac gtc gtc atg gtc ggc cgt
cgc gag gag 6563 Ile Ala Ala Gly Gly Met Asn Val Val Met Val Gly
Arg Arg Glu Glu 435 440 445 aag ctg aac gtg ctc gca ggc gag atc cgc
gag acc tac ggc gtg gag 6611 Lys Leu Asn Val Leu Ala Gly Glu Ile
Arg Glu Thr Tyr Gly Val Glu 450 455 460 acc aag gtc gtg cgc gcc gac
ttt agc cag ccc ggc gct gcc gag acc 6659 Thr Lys Val Val Arg Ala
Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr 465 470 475 gtc ttc gcc gcg
acc gag ggc ctg gac atg ggc ttc atg agc tac gtg 6707 Val Phe Ala
Ala Thr Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val 480 485 490 495
gcc tgc ctg cac agc ttc ggt aag atc cag gac acc ccc tgg gag aag
6755 Ala Cys Leu His Ser Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu
Lys 500 505 510 cac gag gcc atg atc aac gtc aac gtc gtg acc ttc ctc
aag tgc ttc 6803 His Glu Ala Met Ile Asn Val Asn Val Val Thr Phe
Leu Lys Cys Phe 515 520 525 cac cac tac atg cgg atc ttt gcc gcc cag
gac cgc ggc gcc gtg atc 6851 His His Tyr Met Arg Ile Phe Ala Ala
Gln Asp Arg Gly Ala Val Ile 530 535 540 aac gtc tcg tcg atg acc ggc
atc agc tcc agc ccc tgg aac ggc cag 6899 Asn Val Ser Ser Met Thr
Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln 545 550 555 tac ggc gcg ggc
aag gcc ttc atc ctc aag atg acc gag gcc gtg gcc 6947 Tyr Gly Ala
Gly Lys Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala 560 565 570 575
tgc gag tgc gag ggc acc ggc gtc gac gtc gag gtc atc acc ctc ggc
6995 Cys Glu Cys Glu Gly Thr Gly Val Asp Val Glu Val Ile Thr Leu
Gly 580 585 590 acc acc cta acc ccc agc ctg ctg tcc aac ctc ccc ggc
ggc ccg cag 7043 Thr Thr Leu Thr Pro Ser Leu Leu Ser Asn Leu Pro
Gly Gly Pro Gln 595 600 605 ggc gag gcc gtc atg aag atc gcc ctc acc
ccc gag gag tgc gtt gac 7091 Gly Glu Ala Val Met Lys Ile Ala Leu
Thr Pro Glu Glu Cys Val Asp 610 615 620 gag gcc ttt gag aag ctg ggt
aag gag ctc tcc gtc atc gcc ggc cag 7139 Glu Ala Phe Glu Lys Leu
Gly Lys Glu Leu Ser Val Ile Ala Gly Gln 625 630 635 cgc aac aag gac
tcc gtc cac gac tgg aag gca aac cac acc gag gac 7187 Arg Asn Lys
Asp Ser Val His Asp Trp Lys Ala Asn His Thr Glu Asp 640 645 650 655
gag tac atc cgc tac atg ggg tcg ttc tac cgc gac tag aagcttgcgg 7236
Glu Tyr Ile Arg Tyr Met Gly Ser Phe Tyr Arg Asp 660 665 ccgcactcga
gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg 7296
aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
7356 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat 7404
<210> SEQ ID NO 19 <211> LENGTH: 404 <212> TYPE:
PRT <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: Synthetic Construct <400>
SEQUENCE: 19 Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val
Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys
Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile Leu Pro Thr Pro Lys
Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu Leu Gly Ser Val Ser
Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 Glu Ser Asn Gly His
Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80 Asp Ser Val
Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85 90 95 Gln
Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 100 105
110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val
115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu
Val Thr 130 135 140 Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val
Met Met Ile Leu 145 150 155 160 Ser Leu Val Arg Asn Tyr Leu Pro Ser
His Glu Trp Ala Arg Lys Gly 165 170 175 Gly Trp Asn Ile Ala Asp Cys
Val Ser His Ala Tyr Asp Leu Glu Ala 180 185 190 Met His Val Gly Thr
Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 195 200 205 Arg Arg Leu
Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 210 215 220 Arg
Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 225 230
235 240 Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn
Cys 245 250 255 Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu
Thr Leu Lys 260 265 270 Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr
Ala Arg Gly Lys Leu 275 280 285 Cys Asp Arg Asp Ala Val Ala Arg Ala
Leu Glu Ser Gly Arg Leu Ala 290 295 300 Gly Tyr Ala Gly Asp Val Trp
Phe Pro Gln Pro Ala Pro Lys Asp His 305 310 315 320 Pro Trp Arg Thr
Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 325 330 335 Thr Thr
Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 340 345 350
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355
360 365 Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser
Lys 370 375 380 Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe
Lys Lys Ala 385 390 395 400 Ala Glu Asn Ser <210> SEQ ID NO
20 <211> LENGTH: 263 <212> TYPE: PRT <213>
ORGANISM: Artificial <220> FEATURE: <223> OTHER
INFORMATION: Synthetic Construct <400> SEQUENCE: 20 Met Asn
Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20
25 30 Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val
Leu 35 40 45 Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys
Val Val Arg 50 55 60 Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr
Val Phe Ala Ala Thr 65 70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser
Tyr Val Ala Cys Leu His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr
Pro Trp Glu Lys His Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val
Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala
Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr
Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150
155 160 Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu
Gly 165 170 175 Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr
Leu Thr Pro 180 185 190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln
Gly Glu Ala Val Met 195 200 205
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 210
215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp
Ser 225 230 235 240 Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu
Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe Tyr Arg Asp 260
<210> SEQ ID NO 21 <211> LENGTH: 4302 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pCOLA(mod) 3alpha-HSDH
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (303)..(1076) <223> OTHER INFORMATION: 3alpha-HSDH
<400> SEQUENCE: 21 ggggaattgt gagcggataa caattcccct
gtagaaataa ttttgtttaa ctttaataag 60 gagatatacc atgggcagca
gccatcacca tcatcaccac agccaggatc cgaattcgag 120 ctcggcgcgc
ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag 180
taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
240 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
gatatacata 300 tg atg tcc atc atc gtg ata agc ggc tgc gcc acc ggc
att ggt gcc 347 Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr Gly Ile
Gly Ala 1 5 10 15 gct acg cgc aag gtc ctg gag gcg gcc ggt cac cag
atc gta ggc atc 395 Ala Thr Arg Lys Val Leu Glu Ala Ala Gly His Gln
Ile Val Gly Ile 20 25 30 gat ata cgc gat gcg gaa gtg att gcc gat
ctc tcg acg gcc gaa ggt 443 Asp Ile Arg Asp Ala Glu Val Ile Ala Asp
Leu Ser Thr Ala Glu Gly 35 40 45 cga aag cag gcg att gcc gat gta
ctg gcg aag tgc agc aag ggc atg 491 Arg Lys Gln Ala Ile Ala Asp Val
Leu Ala Lys Cys Ser Lys Gly Met 50 55 60 gac ggc ctg gtg ctg tgc
gcc ggc ctg gga ccg cag acc aag gtg ctt 539 Asp Gly Leu Val Leu Cys
Ala Gly Leu Gly Pro Gln Thr Lys Val Leu 65 70 75 ggc aat gtg gtt
tcg gtc aat tat ttt ggc gcg acc gag ctg atg gat 587 Gly Asn Val Val
Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp 80 85 90 95 gcc ttt
ttg cca gcg ctg aaa aaa ggc cat cag ccc gca gcc gtc gtc 635 Ala Phe
Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val 100 105 110
atc tcg tcc gtg gct tcc gcg cat ctg gct ttt gac aag aac cca ctg 683
Ile Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro Leu 115
120 125 gcg ctg gca ctg gaa gcc ggc gag gaa gcc aag gcc cgc gcc att
gtc 731 Ala Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg Ala Ile
Val 130 135 140 gaa cat gcg gga gag cag ggc gga aat ctg gcc tat gcg
ggc agc aag 779 Glu His Ala Gly Glu Gln Gly Gly Asn Leu Ala Tyr Ala
Gly Ser Lys 145 150 155 aat gct ttg acg gtg gct gtg cgc aaa cgc gcc
gcc gcc tgg ggc gag 827 Asn Ala Leu Thr Val Ala Val Arg Lys Arg Ala
Ala Ala Trp Gly Glu 160 165 170 175 gct ggc gtg cgc ctg aac acc atc
gcc ccc ggt gca acc gag act ccc 875 Ala Gly Val Arg Leu Asn Thr Ile
Ala Pro Gly Ala Thr Glu Thr Pro 180 185 190 ttg ctg cag gcg ggc ctg
cag gac ccg cgc tat ggc gaa tcc att gcc 923 Leu Leu Gln Ala Gly Leu
Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala 195 200 205 aag ttc gtt cct
ccc atg ggc cgc cgt gcc gag ccg tcc gag atg gcg 971 Lys Phe Val Pro
Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala 210 215 220 tcg gtc
atc gcc ttt ttg atg agc ccg gcc gca agc tat gtg cat ggc 1019 Ser
Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly 225 230
235 gcg cag atc gtc att gat ggc ggc att gat gcg gtg atg cgc ccg aca
1067 Ala Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg Pro
Thr 240 245 250 255 cag ttc tga gaattcgagc tccgtcgaca agcttgcggc
cgcactcgag 1116 Gln Phe caccaccacc accaccactg agatccggct gctaacaaag
cccgaaagga agctgagttg 1176 gctgctgcca ccgctgagca ataactagca
taaccccttg gggcctctaa acgggtcttg 1236 aggggttttt tgctgaaacc
tcaggcattt gagaagcaca cggtcacact gcttccggta 1296 gtcaataaac
cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa 1356
ccgacgacaa gctgacgacc gggtctccgc aagtggcact tttcggggaa atgtgcgcgg
1416 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
tgaattaatt 1476 cttacgcccc gccctgccac tcatcgcagt actgttgtaa
ttcattaagc attctgccga 1536 catggaagcc atcacagacg gcatgatgaa
cctgaatcgc cagcggcatc agcaccttgt 1596 cgccttgcgt ataatatttg
cctatggtga aaacgggggc gaagaagttg tccatattgg 1656 ccacgtttaa
atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat 1716
tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
1776 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
gatgaaaacg 1836 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
actatcccat atcaccagct 1896 caccgtcttt cattgccata cggaattccg
gatgagcatt catcaggcgg gcaagaatgt 1956 gaataaaggc cggataaaac
ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa 2016 tatccagctg
aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat 2076
gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
2136 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
atgagcggat 2196 acatatttga atgtatttag aaaaataaac aaataggcat
gctagcgcag aaacgtccta 2256 gaagatgcca ggaggatact tagcagagag
acaataaggc cggagcgaag ccgtttttcc 2316 ataggctccg cccccctgac
gaacatcacg aaatctgacg ctcaaatcag tggtggcgaa 2376 acccgacagg
actataaaga taccaggcgt ttccccctga tggctccctc ttgcgctctc 2436
ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc accacgtccc
2496 gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
gttcagcccg 2556 actgctgcgc cttatccggt aactatcatc ttgagtccaa
cccggaaaga cacgacaaaa 2616 cgccactggc agcagccatt ggtaactgag
aattagtgga tttagatatc gagagtcttg 2676 aagtggtggc ctaacagagg
ctacactgaa aggacagtat ttggtatctg cgctccacta 2736 aagccagtta
ccaggttaag cagttcccca actgacttaa ccttcgatca aaccgcctcc 2796
ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag gatctcaaga
2856 agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
atctcttcaa 2916 atgtagcacc tgaagtcagc cccatacgat ataagttgta
attctcatgt tagtcatgcc 2976 ccgcgcccac cggaaggagc tgactgggtt
gaaggctctc aagggcatcg gtcgagatcc 3036 cggtgcctaa tgagtgagct
aacttacatt aattgcgttg cgctcactgc ccgctttcca 3096 gtcgggaaac
ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3156
tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
3216 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
gtttgcccca 3276 gcaggcgaaa atcctgtttg atggtggtta acggcgggat
ataacatgag ctgtcttcgg 3336 tatcgtcgta tcccactacc gagatgtccg
caccaacgcg cagcccggac tcggtaatgg 3396 cgcgcattgc gcccagcgcc
atctgatcgt tggcaaccag catcgcagtg ggaacgatgc 3456 cctcattcag
catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc 3516
gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
3576 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
tgacccaatg 3636 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg
ggagaaaata atactgttga 3696 tgggtgtctg gtcagagaca tcaagaaata
acgccggaac attagtgcag gcagcttcca 3756 cagcaatggc atcctggtca
tccagcggat agttaatgat cagcccactg acgcgttgcg 3816 cgagaagatt
gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca 3876
ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
3936 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
ttgcccgcca 3996 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc
catcgccgct tccacttttt 4056 cccgcgtttt cgcagaaacg tggctggcct
ggttcaccac gcgggaaacg gtctgataag 4116 agacaccggc atactctgcg
acatcgtata acgttactgg tttcacattc accaccctga 4176 attgactctc
ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg 4236
tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
4296 actata 4302 <210> SEQ ID NO 22 <211> LENGTH: 257
<212> TYPE: PRT <213> ORGANISM: Artificial <220>
FEATURE: <223> OTHER INFORMATION: Synthetic Construct
<400> SEQUENCE: 22 Met Ser Ile Ile Val Ile Ser Gly Cys Ala
Thr Gly Ile Gly Ala Ala 1 5 10 15 Thr Arg Lys Val Leu Glu Ala Ala
Gly His Gln Ile Val Gly Ile Asp 20 25 30 Ile Arg Asp Ala Glu Val
Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg 35 40 45 Lys Gln Ala Ile
Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp 50 55 60 Gly Leu
Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly 65 70 75 80
Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala 85
90 95 Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val
Ile 100 105 110 Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn
Pro Leu Ala 115 120 125 Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala
Arg Ala Ile Val Glu 130 135 140 His Ala Gly Glu Gln Gly Gly Asn Leu
Ala Tyr Ala Gly Ser Lys Asn 145 150 155 160 Ala Leu Thr Val Ala Val
Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala
165 170 175 Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr
Pro Leu 180 185 190 Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu
Ser Ile Ala Lys 195 200 205 Phe Val Pro Pro Met Gly Arg Arg Ala Glu
Pro Ser Glu Met Ala Ser 210 215 220 Val Ile Ala Phe Leu Met Ser Pro
Ala Ala Ser Tyr Val His Gly Ala 225 230 235 240 Gln Ile Val Ile Asp
Gly Gly Ile Asp Ala Val Met Arg Pro Thr Gln 245 250 255 Phe
<210> SEQ ID NO 23 <211> LENGTH: 29 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 23
cgatcatatg gcaaaggtcc tgtgcgttc 29 <210> SEQ ID NO 24
<211> LENGTH: 29 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 24 gctagaattc tcagccgcct tcttgaact 29
<210> SEQ ID NO 25 <211> LENGTH: 768 <212> TYPE:
DNA <213> ORGANISM: Escherichia coli <220> FEATURE:
<221> NAME/KEY: CDS <222> LOCATION: (1)..(768)
<400> SEQUENCE: 25 gtg ttt aat tct gac aac ctg aga ctc gac
gga aaa tgc gcc atc atc 48 Val Phe Asn Ser Asp Asn Leu Arg Leu Asp
Gly Lys Cys Ala Ile Ile 1 5 10 15 aca ggt gcg ggt gca ggt att ggt
aaa gaa atc gcc att aca ttc gcg 96 Thr Gly Ala Gly Ala Gly Ile Gly
Lys Glu Ile Ala Ile Thr Phe Ala 20 25 30 aca gct ggc gca tct gtg
gtg gtc agt gat att aac gcc gac gca gct 144 Thr Ala Gly Ala Ser Val
Val Val Ser Asp Ile Asn Ala Asp Ala Ala 35 40 45 aac cat gtt gta
gac gaa att caa caa ctg ggt ggt cag gca ttt gcc 192 Asn His Val Val
Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 50 55 60 tgc cgt
tgt gat att act tcc gaa cag gaa ctc tct gca ctg gca gac 240 Cys Arg
Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 65 70 75 80
ttt gct atc agt aag ctg ggt aaa gtt gat att ctg gtt aac aac gcc 288
Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 85
90 95 ggt ggc ggt gga cct aaa ccg ttt gat atg cca atg gcg gat ttt
cgc 336 Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe
Arg 100 105 110 cgt gct tat gaa ctg aat gtg ttt tct ttt ttc cat ctg
tca caa ctt 384 Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu
Ser Gln Leu 115 120 125 gtt gcg cca gaa atg gaa aaa aat ggc ggt ggc
gtt att ctg acc atc 432 Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly
Val Ile Leu Thr Ile 130 135 140 act tct atg gcg gca gaa aat aaa aat
ata aac atg act tcc tat gca 480 Thr Ser Met Ala Ala Glu Asn Lys Asn
Ile Asn Met Thr Ser Tyr Ala 145 150 155 160 tca tct aaa gct gcg gcc
agt cat ctg gtc aga aat atg gcg ttt gac 528 Ser Ser Lys Ala Ala Ala
Ser His Leu Val Arg Asn Met Ala Phe Asp 165 170 175 cta ggt gaa aaa
aat att cgg gta aat ggc att gcg ccg ggg gca ata 576 Leu Gly Glu Lys
Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 180 185 190 tta acc
gat gcc ctg aaa tcc gtt att aca cca gaa att gaa caa aaa 624 Leu Thr
Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 195 200 205
atg tta cag cac acg ccg atc aga cgt ctg ggc caa ccg caa gat att 672
Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 210
215 220 gct aac gca gcg ctg ttc ctt tgc tcg cct gct gcg agc tgg gta
agc 720 Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val
Ser 225 230 235 240 gga caa att ctc acc gtc tcc ggt ggt ggg gta cag
gag ctc aat taa 768 Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln
Glu Leu Asn 245 250 255 <210> SEQ ID NO 26 <211>
LENGTH: 255 <212> TYPE: PRT <213> ORGANISM: Escherichia
coli <400> SEQUENCE: 26 Val Phe Asn Ser Asp Asn Leu Arg Leu
Asp Gly Lys Cys Ala Ile Ile 1 5 10 15 Thr Gly Ala Gly Ala Gly Ile
Gly Lys Glu Ile Ala Ile Thr Phe Ala 20 25 30 Thr Ala Gly Ala Ser
Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala 35 40 45 Asn His Val
Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 50 55 60 Cys
Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 65 70
75 80 Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn
Ala 85 90 95 Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala
Asp Phe Arg 100 105 110 Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe
His Leu Ser Gln Leu 115 120 125 Val Ala Pro Glu Met Glu Lys Asn Gly
Gly Gly Val Ile Leu Thr Ile 130 135 140 Thr Ser Met Ala Ala Glu Asn
Lys Asn Ile Asn Met Thr Ser Tyr Ala 145 150 155 160 Ser Ser Lys Ala
Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 165 170 175 Leu Gly
Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 180 185 190
Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 195
200 205 Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp
Ile 210 215 220 Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser
Trp Val Ser 225 230 235 240 Gly Gln Ile Leu Thr Val Ser Gly Gly Gly
Val Gln Glu Leu Asn 245 250 255 <210> SEQ ID NO 27
<211> LENGTH: 53 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 27 aaaaaagctt ataattatcc ttataggacg
tcatggtgcg cccagatagg gtg 53 <210> SEQ ID NO 28 <211>
LENGTH: 60 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 28 cagattgtac aaatgtggtg ataacagata
agtcgtcatg tttaacttac ctttctttgt 60 <210> SEQ ID NO 29
<211> LENGTH: 49 <212> TYPE: DNA <213> ORGANISM:
Artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 29 tgaacgcaag tttctaattt cggtttccta
tcgatagagg aaagtgtct 49 <210> SEQ ID NO 30 <211>
LENGTH: 29 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 30 cctgcactac accggccgtc accgcctgc 29
<210> SEQ ID NO 31 <211> LENGTH: 23 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 31
gctcgaattc tcagaccgcc ttc 23 <210> SEQ ID NO 32 <211>
LENGTH: 27 <212> TYPE: DNA <213> ORGANISM: Artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 32 ttaattgagc tcctgtaccc caccacc 27
<210> SEQ ID NO 33 <211> LENGTH: 30 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 33 gtgtttaatt ctgacaacct gagactcgac 30
<210> SEQ ID NO 34 <211> LENGTH: 1266 <212> TYPE:
DNA <213> ORGANISM: Artificial <220> FEATURE:
<223> OTHER INFORMATION: FDH D221G Mutant with His-Tag
<220> FEATURE: <221> NAME/KEY: CDS <222>
LOCATION: (1)..(1266) <400> SEQUENCE: 34 atg gca aag gtc ctg
tgc gtt ctt tac gat gat ccg gtc gac ggc tac 48 Met Ala Lys Val Leu
Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 1 5 10 15 ccg aag acc
tat gcc cgc gac gat ctt ccg aag atc gac cac tat ccg 96 Pro Lys Thr
Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 20 25 30 ggc
ggc cag atc ttg ccg acg ccg aag gcc atc gac ttc acg ccc ggg 144 Gly
Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 35 40
45 cag ttg ctc ggc tcc gtc tcc ggc gag ctc ggc ctg cgc gaa tat ctc
192 Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu
50 55 60 gaa tcc aac ggc cac acc ctg gtc gtg acc tcc gac aag gac
ggc ccc 240 Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp
Gly Pro 65 70 75 80 gac tcg gtg ttc gag cgc gag ctg gtc gat gcg gat
gtc gtc atc tcc 288 Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp
Val Val Ile Ser 85 90 95 cag ccc ttc tgg ccg gcc tat ctg acg ccc
gag cgc atc gcc aag gcc 336 Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro
Glu Arg Ile Ala Lys Ala 100 105 110 aag aac ctg aag ctc gcg ctc acc
gcc ggc atc ggt tcc gac cac gtc 384 Lys Asn Leu Lys Leu Ala Leu Thr
Ala Gly Ile Gly Ser Asp His Val 115 120 125 gat ctt cag tcg gct atc
gac cgc aac gtc acc gtg gcg gaa gtc acc 432 Asp Leu Gln Ser Ala Ile
Asp Arg Asn Val Thr Val Ala Glu Val Thr 130 135 140 tac tgc aac tcg
atc agc gtc gcc gag cat gtg gtg atg atg atc ctg 480 Tyr Cys Asn Ser
Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 145 150 155 160 tcg
ctg gtg cgc aac tat ctg ccc tcg cac gaa tgg gcg cgg aag ggc 528 Ser
Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 165 170
175 ggc tgg aac atc gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg
576 Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala
180 185 190 atg cat gtc ggc acc gtg gcc gcc ggc cgc atc ggt ctc gcg
gtg ctg 624 Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala
Val Leu 195 200 205 cgc cgt ctg gcg ccg ttc gac gtg cac ctg cac tac
acc ggc cgt cac 672 Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr
Thr Gly Arg His 210 215 220 cgc ctg ccg gaa tcg gtc gag aag gag ctc
aac ctc acc tgg cac gcg 720 Arg Leu Pro Glu Ser Val Glu Lys Glu Leu
Asn Leu Thr Trp His Ala 225 230 235 240 acc cgc gag gac atg tat ccg
gtt tgc gac gtg gtg acg ctg aac tgc 768 Thr Arg Glu Asp Met Tyr Pro
Val Cys Asp Val Val Thr Leu Asn Cys 245 250 255 ccg ctg cac ccc gaa
acc gag cac atg atc aat gac gag acg ctg aag 816 Pro Leu His Pro Glu
Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 260 265 270 ctg ttc aag
cgt ggc gcc tac atc gtc aac acc gcc cgc ggc aag ctg 864 Leu Phe Lys
Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 275 280 285 tgc
gac cgc gat gcc gtg gca cgt gcg ctc gaa tcc ggc cgg ctg gcc 912 Cys
Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 290 295
300 ggc tat gcc ggc gac gtg tgg ttc ccg cag ccg gcg ccg aag gac cac
960 Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His
305 310 315 320 ccc tgg cgg acg atg ccc tat aac ggc atg acc ccg cac
atc tcc ggc 1008 Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro
His Ile Ser Gly 325 330 335 acc acg ctg acc gcg cag gcg cgt tat gcg
gcg ggc acc cgc gag atc 1056 Thr Thr Leu Thr Ala Gln Ala Arg Tyr
Ala Ala Gly Thr Arg Glu Ile 340 345 350 ctg gag tgc ttc ttc gag ggc
cgt ccg atc cgc gac gaa tac ctc atc 1104 Leu Glu Cys Phe Phe Glu
Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355 360 365 gtg cag ggc ggc
gct ctt gcc ggc acc ggc gcg cat tcc tac tcg aag 1152 Val Gln Gly
Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 370 375 380 ggc
aat gcc acc ggc ggt tcg gaa gag gcc gcc aag ttc aag aag gcg 1200
Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 385
390 395 400 gct gag aat tcg agc tcc gtc gac aag ctt gcg gcc gca ctc
gag cac 1248 Ala Glu Asn Ser Ser Ser Val Asp Lys Leu Ala Ala Ala
Leu Glu His 405 410 415 cac cac cac cac cac tga 1266 His His His
His His 420 <210> SEQ ID NO 35 <211> LENGTH: 421
<212> TYPE: PRT <213> ORGANISM: Artificial <220>
FEATURE: <223> OTHER INFORMATION: Synthetic Construct
<400> SEQUENCE: 35 Met Ala Lys Val Leu Cys Val Leu Tyr Asp
Asp Pro Val Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg Asp Asp
Leu Pro Lys Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile Leu Pro
Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu Leu Gly
Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 Glu Ser
Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80
Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85
90 95 Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys
Ala 100 105 110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser
Asp His Val 115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr
Val Ala Glu Val Thr 130 135 140 Tyr Cys Asn Ser Ile Ser Val Ala Glu
His Val Val Met Met Ile Leu 145 150 155 160 Ser Leu Val Arg Asn Tyr
Leu Pro Ser His Glu Trp Ala Arg Lys Gly 165 170 175 Gly Trp Asn Ile
Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 180 185 190 Met His
Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 195 200 205
Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 210
215 220 Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His
Ala 225 230 235 240 Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val
Thr Leu Asn Cys 245 250 255 Pro Leu His Pro Glu Thr Glu His Met Ile
Asn Asp Glu Thr Leu Lys 260 265 270 Leu Phe Lys Arg Gly Ala Tyr Ile
Val Asn Thr Ala Arg Gly Lys Leu 275 280 285 Cys Asp Arg Asp Ala Val
Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 290 295 300 Gly Tyr Ala Gly
Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 305 310 315 320 Pro
Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 325 330
335 Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile
340 345 350 Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr
Leu Ile 355 360 365 Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His
Ser Tyr Ser Lys 370 375 380 Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala
Ala Lys Phe Lys Lys Ala 385 390 395 400 Ala Glu Asn Ser Ser Ser Val
Asp Lys Leu Ala Ala Ala Leu Glu His 405 410 415 His His His His His
420 <210> SEQ ID NO 36 <211> LENGTH: 401 <212>
TYPE: PRT <213> ORGANISM: Mycobacterium vaccae <400>
SEQUENCE: 36 Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val
Asp Gly Tyr 1 5 10 15 Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys
Ile Asp His Tyr Pro 20 25 30 Gly Gly Gln Ile Leu Pro Thr Pro Lys
Ala Ile Asp Phe Thr Pro Gly 35 40 45 Gln Leu Leu Gly Ser Val Ser
Gly Glu Leu Gly Leu Arg Glu Tyr Leu 50 55 60 Glu Ser Asn Gly His
Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 65 70 75 80 Asp Ser Val
Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 85 90 95 Gln
Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 100 105
110 Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val
115 120 125 Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu
Val Thr 130 135 140
Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 145
150 155 160 Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg
Lys Gly 165 170 175 Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr
Asp Leu Glu Ala 180 185 190 Met His Val Gly Thr Val Ala Ala Gly Arg
Ile Gly Leu Ala Val Leu 195 200 205 Arg Arg Leu Ala Pro Phe Asp Val
His Leu His Tyr Thr Asp Arg His 210 215 220 Arg Leu Pro Glu Ser Val
Glu Lys Glu Leu Asn Leu Thr Trp His Ala 225 230 235 240 Thr Arg Glu
Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 245 250 255 Pro
Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 260 265
270 Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu
275 280 285 Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg
Leu Ala 290 295 300 Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala
Pro Lys Asp His 305 310 315 320 Pro Trp Arg Thr Met Pro Tyr Asn Gly
Met Thr Pro His Ile Ser Gly 325 330 335 Thr Thr Leu Thr Ala Gln Ala
Arg Tyr Ala Ala Gly Thr Arg Glu Ile 340 345 350 Leu Glu Cys Phe Phe
Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 355 360 365 Val Gln Gly
Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 370 375 380 Gly
Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 385 390
395 400 Val <210> SEQ ID NO 37 <211> LENGTH: 263
<212> TYPE: PRT <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: derived from Collinsella
aerofaciens (G39D) <400> SEQUENCE: 37 Met Asn Leu Arg Glu Lys
Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu Gly Val
Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30 Met Asn
Val Val Met Val Asp Arg Arg Glu Glu Lys Leu Asn Val Leu 35 40 45
Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 50
55 60 Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala
Thr 65 70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys
Leu His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys
His Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val Thr Phe Leu Lys
Cys Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln Asp Arg
Gly Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr Gly Ile Ser Ser
Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160 Ala Phe
Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165 170 175
Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 180
185 190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val
Met 195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala
Phe Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln
Arg Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp Lys Ala Asn His
Thr Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe Tyr Arg
Asp 260 <210> SEQ ID NO 38 <211> LENGTH: 263
<212> TYPE: PRT <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: derived from Collinsella
aerofaciens (G39D R40L) <400> SEQUENCE: 38 Met Asn Leu Arg
Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu
Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30
Met Asn Val Val Met Val Asp Leu Arg Glu Glu Lys Leu Asn Val Leu 35
40 45 Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val
Arg 50 55 60 Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe
Ala Ala Thr 65 70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val
Ala Cys Leu His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp
Glu Lys His Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val Thr Phe
Leu Lys Cys Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln
Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr Gly Ile
Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165
170 175 Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr
Pro 180 185 190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu
Ala Val Met 195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp
Glu Ala Phe Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala
Gly Gln Arg Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp Lys Ala
Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe
Tyr Arg Asp 260 <210> SEQ ID NO 39 <211> LENGTH: 263
<212> TYPE: PRT <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: derived from Collinsella
aerofaciens (G39D R40I) <400> SEQUENCE: 39 Met Asn Leu Arg
Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu
Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30
Met Asn Val Val Met Val Asp Ile Arg Glu Glu Lys Leu Asn Val Leu 35
40 45 Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val
Arg 50 55 60 Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe
Ala Ala Thr 65 70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val
Ala Cys Leu His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp
Glu Lys His Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val Thr Phe
Leu Lys Cys Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln
Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr Gly Ile
Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165
170 175 Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr
Pro 180 185 190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu
Ala Val Met 195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp
Glu Ala Phe Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala
Gly Gln Arg Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp Lys Ala
Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe
Tyr Arg Asp 260 <210> SEQ ID NO 40 <211> LENGTH: 263
<212> TYPE: PRT <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: derived from Collinsella
aerofaciens <400> SEQUENCE: 40 Met Asn Leu Arg Glu Lys Tyr
Gly Glu Trp Gly Leu Ile Leu Gly Ala 1 5 10 15 Thr Glu Gly Val Gly
Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 20 25 30
Met Asn Val Val Met Val Asp Val Arg Glu Glu Lys Leu Asn Val Leu 35
40 45 Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val
Arg 50 55 60 Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe
Ala Ala Thr 65 70 75 80 Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val
Ala Cys Leu His Ser 85 90 95 Phe Gly Lys Ile Gln Asp Thr Pro Trp
Glu Lys His Glu Ala Met Ile 100 105 110 Asn Val Asn Val Val Thr Phe
Leu Lys Cys Phe His His Tyr Met Arg 115 120 125 Ile Phe Ala Ala Gln
Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 130 135 140 Thr Gly Ile
Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 145 150 155 160
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 165
170 175 Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr
Pro 180 185 190 Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu
Ala Val Met 195 200 205 Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp
Glu Ala Phe Glu Lys 210 215 220 Leu Gly Lys Glu Leu Ser Val Ile Ala
Gly Gln Arg Asn Lys Asp Ser 225 230 235 240 Val His Asp Trp Lys Ala
Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 245 250 255 Met Gly Ser Phe
Tyr Arg Asp 260 <210> SEQ ID NO 41 <211> LENGTH: 26
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: PCR Primer <400>
SEQUENCE: 41 cgtcgtcatg gtcgaccgtc gcgagg 26 <210> SEQ ID NO
42 <211> LENGTH: 26 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer <400> SEQUENCE: 42 cctcgcgacg
gtcgaccatg acgacg 26 <210> SEQ ID NO 43 <211> LENGTH:
26 <212> TYPE: DNA <213> ORGANISM: artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
<400> SEQUENCE: 43 cgtcgtcatg gtcgacctgc gcgagg 26
<210> SEQ ID NO 44 <211> LENGTH: 31 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 44
ccgccgcatc cataccgcca gttgtttacc c 31 <210> SEQ ID NO 45
<211> LENGTH: 26 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer <400> SEQUENCE: 45 cgtcgtcatg gtcgacattc gcgagg 26
<210> SEQ ID NO 46 <211> LENGTH: 26 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 46
cgtcgtcatg gtcgacgttc gcgagg 26 <210> SEQ ID NO 47
<211> LENGTH: 786 <212> TYPE: DNA <213> ORGANISM:
Bacillus subtilis <400> SEQUENCE: 47 atgtatccgg atttaaaagg
aaaagtcgtc gctattacag gagctgcttc agggctcgga 60 aaggcgatgg
ccattcgctt cggcaaggag caggcaaaag tggttatcaa ctattatagt 120
aataaacaag atccgaacga ggtaaaagaa gaggtcatca aggcgggcgg tgaagctgtt
180 gtcgtccaag gagatgtcac gaaagaggaa gatgtaaaaa atatcgtgca
aacggcaatt 240 aaggagttcg gcacactcga tattatgatt aataatgccg
gtcttgaaaa tcctgtgcca 300 tctcacgaaa tgccgctcaa ggattgggat
aaagtcatcg gcacgaactt aacgggtgcc 360 tttttaggaa gccgtgaagc
gattaaatat ttcgtagaaa acgatatcaa gggaaatgtc 420 attaacatgt
ccagtgtgca cgaagtgatt ccttggccgt tatttgtcca ctatgcggca 480
agtaaaggcg ggataaagct gatgacagaa acattagcgt tggaatacgc gccgaagggc
540 attcgcgtca ataatattgg gccaggtgcg atcaacacgc caatcaatgc
tgaaaaattc 600 gctgacccta aacagaaagc tgatgtagaa agcatgattc
caatgggata tatcggcgaa 660 ccggaggaga tcgccgcagt agcagcctgg
cttgcttcga aggaagccag ctacgtcaca 720 ggcatcacgt tattcgcgga
cggcggtatg acacaatatc cttcattcca ggcaggccgc 780 ggttaa 786
<210> SEQ ID NO 48 <211> LENGTH: 261 <212> TYPE:
PRT <213> ORGANISM: Bacillus subtilis <400> SEQUENCE:
48 Met Tyr Pro Asp Leu Lys Gly Lys Val Val Ala Ile Thr Gly Ala Ala
1 5 10 15 Ser Gly Leu Gly Lys Ala Met Ala Ile Arg Phe Gly Lys Glu
Gln Ala 20 25 30 Lys Val Val Ile Asn Tyr Tyr Ser Asn Lys Gln Asp
Pro Asn Glu Val 35 40 45 Lys Glu Glu Val Ile Lys Ala Gly Gly Glu
Ala Val Val Val Gln Gly 50 55 60 Asp Val Thr Lys Glu Glu Asp Val
Lys Asn Ile Val Gln Thr Ala Ile 65 70 75 80 Lys Glu Phe Gly Thr Leu
Asp Ile Met Ile Asn Asn Ala Gly Leu Glu 85 90 95 Asn Pro Val Pro
Ser His Glu Met Pro Leu Lys Asp Trp Asp Lys Val 100 105 110 Ile Gly
Thr Asn Leu Thr Gly Ala Phe Leu Gly Ser Arg Glu Ala Ile 115 120 125
Lys Tyr Phe Val Glu Asn Asp Ile Lys Gly Asn Val Ile Asn Met Ser 130
135 140 Ser Val His Glu Val Ile Pro Trp Pro Leu Phe Val His Tyr Ala
Ala 145 150 155 160 Ser Lys Gly Gly Ile Lys Leu Met Thr Glu Thr Leu
Ala Leu Glu Tyr 165 170 175 Ala Pro Lys Gly Ile Arg Val Asn Asn Ile
Gly Pro Gly Ala Ile Asn 180 185 190 Thr Pro Ile Asn Ala Glu Lys Phe
Ala Asp Pro Lys Gln Lys Ala Asp 195 200 205 Val Glu Ser Met Ile Pro
Met Gly Tyr Ile Gly Glu Pro Glu Glu Ile 210 215 220 Ala Ala Val Ala
Ala Trp Leu Ala Ser Lys Glu Ala Ser Tyr Val Thr 225 230 235 240 Gly
Ile Thr Leu Phe Ala Asp Gly Gly Met Thr Gln Tyr Pro Ser Phe 245 250
255 Gln Ala Gly Arg Gly 260 <210> SEQ ID NO 49 <211>
LENGTH: 7404 <212> TYPE: DNA <213> ORGANISM: artificial
<220> FEATURE: <223> OTHER INFORMATION: Vector pFr7(D)
<400> SEQUENCE: 49 atccggatat agttcctcct ttcagcaaaa
aacccctcaa gacccgttta gaggccccaa 60 ggggttatgc tagttattgc
tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120 tgttagcagc
cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttct 180
agtcgcggta gaacgacccc atgtagcgga tgtactcgtc ctcggtgtgg tttgccttcc
240 agtcgtggac ggagtccttg ttgcgctggc cggcgatgac ggagagctcc
ttacccagct 300 tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc
gatcttcatg acggcctcgc 360 cctgcgggcc gccggggagg ttggacagca
ggctgggggt tagggtggtg ccgagggtga 420 tgacctcgac gtcgacgccg
gtgccctcgc actcgcaggc cacggcctcg gtcatcttga 480 ggatgaaggc
cttgcccgcg ccgtactggc cgttccaggg gctggagctg atgccggtca 540
tcgacgagac gttgatcacg gcgccgcggt cctgggcggc aaagatccgc atgtagtggt
600 ggaagcactt gaggaaggtc acgacgttga cgttgatcat ggcctcgtgc
ttctcccagg 660 gggtgtcctg gatcttaccg aagctgtgca ggcaggccac
gtagctcatg aagcccatgt 720 ccaggccctc ggtcgcggcg aagacggtct
cggcagcgcc gggctggcta aagtcggcgc 780 gcacgacctt ggtctccacg
ccgtaggtct cgcggatctc gcctgcgagc acgttcagct 840 tctcctcgcg
acggtcgacc atgacgacgt tcatgccgcc ggcggcgatc ttctcgcaga 900
acgccttgcc gacgccctcg gtcgcgccca ggatcaggcc ccactcaccg tacttctccc
960 tcaggttcat gtatatctcc tttcacgaat tctcagccgc cttcttgaac
ttggcggcct 1020 cttccgaacc gccggtggca ttgcccttcg agtaggaatg
cgcgccggtg ccggcaagag 1080 cgccgccctg cacgatgagg tattcgtcgc
ggatcggacg gccctcgaag aagcactcca 1140 ggatctcgcg ggtgcccgcc
gcataacgcg cctgcgcggt cagcgtggtg ccggagatgt 1200 gcggggtcat
gccgttatag ggcatcgtcc gccaggggtg gtccttcggc gccggctgcg 1260
ggaaccacac gtcgccggca tagccggcca gccggccgga ttcgagcgca cgtgccacgg
1320 catcgcggtc gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca
cgcttgaaca 1380 gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg
gtgcagcggg cagttcagcg 1440 tcaccacgtc gcaaaccgga tacatgtcct
cgcgggtcgc gtgccaggtg aggttgagct 1500 ccttctcgac cgattccggc
aggcggtgac ggccggtgta gtgcaggtgc acgtcgaacg 1560 gcgccagacg
gcgcagcacc gcgagaccga tgcggccggc ggccacggtg ccgacatgca 1620
tcgcctcgag gtcgtaggcg tgggagacgc agtcggcgat gttccagccg cccttccgcg
1680 cccattcgtg cgagggcaga tagttgcgca ccagcgacag gatcatcatc
accacatgct 1740 cggcgacgct gatcgagttg cagtaggtga cttccgccac
ggtgacgttg cggtcgatag 1800 ccgactgaag atcgacgtgg tcggaaccga
tgccggcggt gagcgcgagc ttcaggttct 1860 tggccttggc gatgcgctcg
ggcgtcagat aggccggcca gaagggctgg gagatgacga 1920 catccgcatc
gaccagctcg cgctcgaaca ccgagtcggg gccgtccttg tcggaggtca 1980
cgaccagggt gtggccgttg gattcgagat attcgcgcag gccgagctcg ccggagacgg
2040 agccgagcaa ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag
atctggccgc 2100 ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata
ggtcttcggg tagccgtcga 2160 ccggatcatc gtaaagaacg cacaggacct
ttgccatcat atgtatatct ccttcttaaa 2220 gttaaacaaa attatttcta
gaggggaatt gttatccgct cacaattccc ctatagtgag 2280 tcgtattaat
ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc 2340
atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
2400 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
ggtggcaggc 2460 cccgtggccg ggggactgtt gggcgccatc tccttgcatg
caccattcct tgcggcggcg 2520 gtgctcaacg gcctcaacct actactgggc
tgcttcctaa tgcaggagtc gcataaggga 2580 gagcgtcgag atcccggaca
ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 2640 gcgcccggaa
gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 2700
cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
2760 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
attacattcc 2820 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
ctgattggcg ttgccacctc 2880 cagtctggcc ctgcacgcgc cgtcgcaaat
tgtcgcggcg attaaatctc gcgccgatca 2940 actgggtgcc agcgtggtgg
tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 3000 ggcggtgcac
aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 3060
tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
3120 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
gtacgcgact 3180 gggcgtggag catctggtcg cattgggtca ccagcaaatc
gcgctgttag cgggcccatt 3240 aagttctgtc tcggcgcgtc tgcgtctggc
tggctggcat aaatatctca ctcgcaatca 3300 aattcagccg atagcggaac
gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 3360 catgcaaatg
ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 3420
ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
3480 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
taaccaccat 3540 caaacaggat tttcgcctgc tggggcaaac cagcgtggac
cgcttgctgc aactctctca 3600 gggccaggcg gtgaagggca atcagctgtt
gcccgtctca ctggtgaaaa gaaaaaccac 3660 cctggcgccc aatacgcaaa
ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 3720 ggcacgacag
gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 3780
agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
3840 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
gtcttcttta 3900 tcatgcaact cgtaggacag gtgccggcag cgctctgggt
cattttcggc gaggaccgct 3960 ttcgctggag cgcgacgatg atcggcctgt
cgcttgcggt attcggaatc ttgcacgccc 4020 tcgctcaagc cttcgtcact
ggtcccgcca ccaaacgttt cggcgagaag caggccatta 4080 tcgccggcat
ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 4140
taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
4200 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
gtcttcggtt 4260 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
ctgcaccatt atgttccgga 4320 tctgcatcgc aggatgctgc tggctaccct
gtggaacacc tacatctgta ttaacgaagc 4380 gctggcattg accctgagtg
atttttctct ggtcccgccg catccatacc gccagttgtt 4440 taccctcaca
acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca 4500
tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
4560 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
agaagccaga 4620 cattaacgct tctggagaaa ctcaacgagc tggacgcgga
tgaacaggca gacatctgtg 4680 aatcgcttca cgaccacgct gatgagcttt
accgcagctg cctcgcgcgt ttcggtgatg 4740 acggtgaaaa cctctgacac
atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 4800 atgccgggag
cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 4860
cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
4920 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca
cagatgcgta 4980 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
tcactgactc gctgcgctcg 5040 gtcgttcggc tgcggcgagc ggtatcagct
cactcaaagg cggtaatacg gttatccaca 5100 gaatcagggg ataacgcagg
aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 5160 cgtaaaaagg
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 5220
aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
5280 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
taccggatac 5340 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
atagctcacg ctgtaggtat 5400 ctcagttcgg tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag 5460 cccgaccgct gcgccttatc
cggtaactat cgtcttgagt ccaacccggt aagacacgac 5520 ttatcgccac
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 5580
gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
5640 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
ttgatccggc 5700 aaacaaacca ccgctggtag cggtggtttt tttgtttgca
agcagcagat tacgcgcaga 5760 aaaaaaggat ctcaagaaga tcctttgatc
ttttctacgg ggtctgacgc tcagtggaac 5820 gaaaactcac gttaagggat
tttggtcatg agattatcaa aaaggatctt cacctagatc 5880 cttttaaatt
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5940
gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
6000 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
cttaccatct 6060 ggccccagtg ctgcaatgat accgcgagac ccacgctcac
cggctccaga tttatcagca 6120 ataaaccagc cagccggaag ggccgagcgc
agaagtggtc ctgcaacttt atccgcctcc 6180 atccagtcta ttaattgttg
ccgggaagct agagtaagta gttcgccagt taatagtttg 6240 cgcaacgttg
ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6300
tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
6360 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
cgcagtgtta 6420 tcactcatgg ttatggcagc actgcataat tctcttactg
tcatgccatc cgtaagatgc 6480 ttttctgtga ctggtgagta ctcaaccaag
tcattctgag aatagtgtat gcggcgaccg 6540 agttgctctt gcccggcgtc
aatacgggat aataccgcgc cacatagcag aactttaaaa 6600 gtgctcatca
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6660
agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
6720 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
gggaataagg 6780 gcgacacgga aatgttgaat actcatactc ttcctttttc
aatattattg aagcatttat 6840 cagggttatt gtctcatgag cggatacata
tttgaatgta tttagaaaaa taaacaaata 6900 ggggttccgc gcacatttcc
ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg 6960 ttaaaattcg
cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 7020
ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
7080 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
aaaaaccgtc 7140 tatcagggcg atggcccact acgtgaacca tcaccctaat
caagtttttt ggggtcgagg 7200 tgccgtaaag cactaaatcg gaaccctaaa
gggagccccc gatttagagc ttgacgggga 7260 aagccggcga acgtggcgag
aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 7320 ctggcaagtg
tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 7380
ctacagggcg cgtcccattc gcca 7404 <210> SEQ ID NO 50
<211> LENGTH: 7404 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: Vector pFr7(DL) <400> SEQUENCE: 50 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gcaagcttct 180 agtcgcggta gaacgacccc atgtagcgga tgtactcgtc
ctcggtgtgg tttgccttcc 240 agtcgtggac ggagtccttg ttgcgctggc
cggcgatgac ggagagctcc ttacccagct 300 tctcaaaggc ctcgtcaacg
cactcctcgg gggtgagggc gatcttcatg acggcctcgc 360 cctgcgggcc
gccggggagg ttggacagca ggctgggggt tagggtggtg ccgagggtga 420
tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc cacggcctcg gtcatcttga
480 ggatgaaggc cttgcccgcg ccgtactggc cgttccaggg gctggagctg
atgccggtca 540 tcgacgagac gttgatcacg gcgccgcggt cctgggcggc
aaagatccgc atgtagtggt 600 ggaagcactt gaggaaggtc acgacgttga
cgttgatcat ggcctcgtgc ttctcccagg 660
gggtgtcctg gatcttaccg aagctgtgca ggcaggccac gtagctcatg aagcccatgt
720 ccaggccctc ggtcgcggcg aagacggtct cggcagcgcc gggctggcta
aagtcggcgc 780 gcacgacctt ggtctccacg ccgtaggtct cgcggatctc
gcctgcgagc acgttcagct 840 tctcctcgcg caggtcgacc atgacgacgt
tcatgccgcc ggcggcgatc ttctcgcaga 900 acgccttgcc gacgccctcg
gtcgcgccca ggatcaggcc ccactcaccg tacttctccc 960 tcaggttcat
gtatatctcc tttcacgaat tctcagccgc cttcttgaac ttggcggcct 1020
cttccgaacc gccggtggca ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag
1080 cgccgccctg cacgatgagg tattcgtcgc ggatcggacg gccctcgaag
aagcactcca 1140 ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt
cagcgtggtg ccggagatgt 1200 gcggggtcat gccgttatag ggcatcgtcc
gccaggggtg gtccttcggc gccggctgcg 1260 ggaaccacac gtcgccggca
tagccggcca gccggccgga ttcgagcgca cgtgccacgg 1320 catcgcggtc
gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca cgcttgaaca 1380
gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg gtgcagcggg cagttcagcg
1440 tcaccacgtc gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg
aggttgagct 1500 ccttctcgac cgattccggc aggcggtgac ggccggtgta
gtgcaggtgc acgtcgaacg 1560 gcgccagacg gcgcagcacc gcgagaccga
tgcggccggc ggccacggtg ccgacatgca 1620 tcgcctcgag gtcgtaggcg
tgggagacgc agtcggcgat gttccagccg cccttccgcg 1680 cccattcgtg
cgagggcaga tagttgcgca ccagcgacag gatcatcatc accacatgct 1740
cggcgacgct gatcgagttg cagtaggtga cttccgccac ggtgacgttg cggtcgatag
1800 ccgactgaag atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc
ttcaggttct 1860 tggccttggc gatgcgctcg ggcgtcagat aggccggcca
gaagggctgg gagatgacga 1920 catccgcatc gaccagctcg cgctcgaaca
ccgagtcggg gccgtccttg tcggaggtca 1980 cgaccagggt gtggccgttg
gattcgagat attcgcgcag gccgagctcg ccggagacgg 2040 agccgagcaa
ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag atctggccgc 2100
ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga
2160 ccggatcatc gtaaagaacg cacaggacct ttgccatcat atgtatatct
ccttcttaaa 2220 gttaaacaaa attatttcta gaggggaatt gttatccgct
cacaattccc ctatagtgag 2280 tcgtattaat ttcgcgggat cgagatctcg
atcctctacg ccggacgcat cgtggccggc 2340 atcaccggcg ccacaggtgc
ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2400 gatcgggctc
gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 2460
cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
2520 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
gcataaggga 2580 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
tttcgcggta tggcatgata 2640 gcgcccggaa gagagtcaat tcagggtggt
gaatgtgaaa ccagtaacgt tatacgatgt 2700 cgcagagtat gccggtgtct
cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2760 cgtttctgcg
aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 2820
caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
2880 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
gcgccgatca 2940 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
ggcgtcgaag cctgtaaagc 3000 ggcggtgcac aatcttctcg cgcaacgcgt
cagtgggctg atcattaact atccgctgga 3060 tgaccaggat gccattgctg
tggaagctgc ctgcactaat gttccggcgt tatttcttga 3120 tgtctctgac
cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 3180
gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
3240 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
ctcgcaatca 3300 aattcagccg atagcggaac gggaaggcga ctggagtgcc
atgtccggtt ttcaacaaac 3360 catgcaaatg ctgaatgagg gcatcgttcc
cactgcgatg ctggttgcca acgatcagat 3420 ggcgctgggc gcaatgcgcg
ccattaccga gtccgggctg cgcgttggtg cggatatctc 3480 ggtagtggga
tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 3540
caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
3600 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
gaaaaaccac 3660 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
gccgattcat taatgcagct 3720 ggcacgacag gtttcccgac tggaaagcgg
gcagtgagcg caacgcaatt aatgtaagtt 3780 agctcactca ttaggcaccg
ggatctcgac cgatgccctt gagagccttc aacccagtca 3840 gctccttccg
gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3900
tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
3960 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
ttgcacgccc 4020 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
cggcgagaag caggccatta 4080 tcgccggcat ggcggcccca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc 4140 taggctggcg gggttgcctt
actggttagc agaatgaatc accgatacgc gagcgaacgt 4200 gaagcgactg
ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 4260
tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
4320 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc 4380 gctggcattg accctgagtg atttttctct ggtcccgccg
catccatacc gccagttgtt 4440 taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca 4500 tcctctctcg tttcatcggt
atcattaccc ccatgaacag aaatccccct tacacggagg 4560 catcagtgac
caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 4620
cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
4680 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
ttcggtgatg 4740 acggtgaaaa cctctgacac atgcagctcc cggagacggt
cacagcttgt ctgtaagcgg 4800 atgccgggag cagacaagcc cgtcagggcg
cgtcagcggg tgttggcggg tgtcggggcg 4860 cagccatgac ccagtcacgt
agcgatagcg gagtgtatac tggcttaact atgcggcatc 4920 agagcagatt
gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 4980
aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5040 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
gttatccaca 5100 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
gccagcaaaa ggccaggaac 5160 cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac 5220 aaaaatcgac gctcaagtca
gaggtggcga aacccgacag gactataaag ataccaggcg 5280 tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5340
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5400 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag 5460 cccgaccgct gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac 5520 ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt 5580 gctacagagt tcttgaagtg
gtggcctaac tacggctaca ctagaaggac agtatttggt 5640 atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5700
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5760 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
tcagtggaac 5820 gaaaactcac gttaagggat tttggtcatg agattatcaa
aaaggatctt cacctagatc 5880 cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct 5940 gacagttacc aatgcttaat
cagtgaggca cctatctcag cgatctgtct atttcgttca 6000 tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6060
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6120 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
atccgcctcc 6180 atccagtcta ttaattgttg ccgggaagct agagtaagta
gttcgccagt taatagtttg 6240 cgcaacgttg ttgccattgc tgcaggcatc
gtggtgtcac gctcgtcgtt tggtatggct 6300 tcattcagct ccggttccca
acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6360 aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6420
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
6480 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
gcggcgaccg 6540 agttgctctt gcccggcgtc aatacgggat aataccgcgc
cacatagcag aactttaaaa 6600 gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg 6660 agatccagtt cgatgtaacc
cactcgtgca cccaactgat cttcagcatc ttttactttc 6720 accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6780
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6840 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
taaacaaata 6900 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
ttgtaaacgt taatattttg 6960 ttaaaattcg cgttaaattt ttgttaaatc
agctcatttt ttaaccaata ggccgaaatc 7020 ggcaaaatcc cttataaatc
aaaagaatag accgagatag ggttgagtgt tgttccagtt 7080 tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7140
tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7200 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
ttgacgggga 7260 aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg 7320 ctggcaagtg tagcggtcac gctgcgcgta
accaccacac ccgccgcgct taatgcgccg 7380 ctacagggcg cgtcccattc gcca
7404 <210> SEQ ID NO 51 <211> LENGTH: 7404 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: Vector pFr7(DI) <400>
SEQUENCE: 51 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gcaagcttct 180 agtcgcggta gaacgacccc
atgtagcgga tgtactcgtc ctcggtgtgg tttgccttcc 240 agtcgtggac
ggagtccttg ttgcgctggc cggcgatgac ggagagctcc ttacccagct 300
tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc gatcttcatg acggcctcgc
360 cctgcgggcc gccggggagg ttggacagca ggctgggggt tagggtggtg
ccgagggtga 420 tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc
cacggcctcg gtcatcttga 480
ggatgaaggc cttgcccgcg ccgtactggc cgttccaggg gctggagctg atgccggtca
540 tcgacgagac gttgatcacg gcgccgcggt cctgggcggc aaagatccgc
atgtagtggt 600 ggaagcactt gaggaaggtc acgacgttga cgttgatcat
ggcctcgtgc ttctcccagg 660 gggtgtcctg gatcttaccg aagctgtgca
ggcaggccac gtagctcatg aagcccatgt 720 ccaggccctc ggtcgcggcg
aagacggtct cggcagcgcc gggctggcta aagtcggcgc 780 gcacgacctt
ggtctccacg ccgtaggtct cgcggatctc gcctgcgagc acgttcagct 840
tctcctcgcg aatgtcgacc atgacgacgt tcatgccgcc ggcggcgatc ttctcgcaga
900 acgccttgcc gacgccctcg gtcgcgccca ggatcaggcc ccactcaccg
tacttctccc 960 tcaggttcat gtatatctcc tttcacgaat tctcagccgc
cttcttgaac ttggcggcct 1020 cttccgaacc gccggtggca ttgcccttcg
agtaggaatg cgcgccggtg ccggcaagag 1080 cgccgccctg cacgatgagg
tattcgtcgc ggatcggacg gccctcgaag aagcactcca 1140 ggatctcgcg
ggtgcccgcc gcataacgcg cctgcgcggt cagcgtggtg ccggagatgt 1200
gcggggtcat gccgttatag ggcatcgtcc gccaggggtg gtccttcggc gccggctgcg
1260 ggaaccacac gtcgccggca tagccggcca gccggccgga ttcgagcgca
cgtgccacgg 1320 catcgcggtc gcacagcttg ccgcgggcgg tgttgacgat
gtaggcgcca cgcttgaaca 1380 gcttcagcgt ctcgtcattg atcatgtgct
cggtttcggg gtgcagcggg cagttcagcg 1440 tcaccacgtc gcaaaccgga
tacatgtcct cgcgggtcgc gtgccaggtg aggttgagct 1500 ccttctcgac
cgattccggc aggcggtgac ggccggtgta gtgcaggtgc acgtcgaacg 1560
gcgccagacg gcgcagcacc gcgagaccga tgcggccggc ggccacggtg ccgacatgca
1620 tcgcctcgag gtcgtaggcg tgggagacgc agtcggcgat gttccagccg
cccttccgcg 1680 cccattcgtg cgagggcaga tagttgcgca ccagcgacag
gatcatcatc accacatgct 1740 cggcgacgct gatcgagttg cagtaggtga
cttccgccac ggtgacgttg cggtcgatag 1800 ccgactgaag atcgacgtgg
tcggaaccga tgccggcggt gagcgcgagc ttcaggttct 1860 tggccttggc
gatgcgctcg ggcgtcagat aggccggcca gaagggctgg gagatgacga 1920
catccgcatc gaccagctcg cgctcgaaca ccgagtcggg gccgtccttg tcggaggtca
1980 cgaccagggt gtggccgttg gattcgagat attcgcgcag gccgagctcg
ccggagacgg 2040 agccgagcaa ctgcccgggc gtgaagtcga tggccttcgg
cgtcggcaag atctggccgc 2100 ccggatagtg gtcgatcttc ggaagatcgt
cgcgggcata ggtcttcggg tagccgtcga 2160 ccggatcatc gtaaagaacg
cacaggacct ttgccatcat atgtatatct ccttcttaaa 2220 gttaaacaaa
attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag 2280
tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
2340 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
cgatggggaa 2400 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
gcgtgggtat ggtggcaggc 2460 cccgtggccg ggggactgtt gggcgccatc
tccttgcatg caccattcct tgcggcggcg 2520 gtgctcaacg gcctcaacct
actactgggc tgcttcctaa tgcaggagtc gcataaggga 2580 gagcgtcgag
atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 2640
gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
2700 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
aggccagcca 2760 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
gcggagctga attacattcc 2820 caaccgcgtg gcacaacaac tggcgggcaa
acagtcgttg ctgattggcg ttgccacctc 2880 cagtctggcc ctgcacgcgc
cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 2940 actgggtgcc
agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 3000
ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
3060 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
tatttcttga 3120 tgtctctgac cagacaccca tcaacagtat tattttctcc
catgaagacg gtacgcgact 3180 gggcgtggag catctggtcg cattgggtca
ccagcaaatc gcgctgttag cgggcccatt 3240 aagttctgtc tcggcgcgtc
tgcgtctggc tggctggcat aaatatctca ctcgcaatca 3300 aattcagccg
atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 3360
catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
3420 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
cggatatctc 3480 ggtagtggga tacgacgata ccgaagacag ctcatgttat
atcccgccgt taaccaccat 3540 caaacaggat tttcgcctgc tggggcaaac
cagcgtggac cgcttgctgc aactctctca 3600 gggccaggcg gtgaagggca
atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 3660 cctggcgccc
aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 3720
ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
3780 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc
aacccagtca 3840 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
acttatgact gtcttcttta 3900 tcatgcaact cgtaggacag gtgccggcag
cgctctgggt cattttcggc gaggaccgct 3960 ttcgctggag cgcgacgatg
atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 4020 tcgctcaagc
cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 4080
tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
4140 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
gagcgaacgt 4200 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
aacatgaatg gtcttcggtt 4260 tccgtgtttc gtaaagtctg gaaacgcgga
agtcagcgcc ctgcaccatt atgttccgga 4320 tctgcatcgc aggatgctgc
tggctaccct gtggaacacc tacatctgta ttaacgaagc 4380 gctggcattg
accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt 4440
taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca
4500 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
tacacggagg 4560 catcagtgac caaacaggaa aaaaccgccc ttaacatggc
ccgctttatc agaagccaga 4620 cattaacgct tctggagaaa ctcaacgagc
tggacgcgga tgaacaggca gacatctgtg 4680 aatcgcttca cgaccacgct
gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 4740 acggtgaaaa
cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 4800
atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
4860 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
atgcggcatc 4920 agagcagatt gtactgagag tgcaccatat atgcggtgtg
aaataccgca cagatgcgta 4980 aggagaaaat accgcatcag gcgctcttcc
gcttcctcgc tcactgactc gctgcgctcg 5040 gtcgttcggc tgcggcgagc
ggtatcagct cactcaaagg cggtaatacg gttatccaca 5100 gaatcagggg
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 5160
cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
5220 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
ataccaggcg 5280 tttccccctg gaagctccct cgtgcgctct cctgttccga
ccctgccgct taccggatac 5340 ctgtccgcct ttctcccttc gggaagcgtg
gcgctttctc atagctcacg ctgtaggtat 5400 ctcagttcgg tgtaggtcgt
tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5460 cccgaccgct
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 5520
ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
5580 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
agtatttggt 5640 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
ttggtagctc ttgatccggc 5700 aaacaaacca ccgctggtag cggtggtttt
tttgtttgca agcagcagat tacgcgcaga 5760 aaaaaaggat ctcaagaaga
tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5820 gaaaactcac
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5880
cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5940 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
atttcgttca 6000 tccatagttg cctgactccc cgtcgtgtag ataactacga
tacgggaggg cttaccatct 6060 ggccccagtg ctgcaatgat accgcgagac
ccacgctcac cggctccaga tttatcagca 6120 ataaaccagc cagccggaag
ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6180 atccagtcta
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6240
cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
6300 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
gttgtgcaaa 6360 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
gtaagttggc cgcagtgtta 6420 tcactcatgg ttatggcagc actgcataat
tctcttactg tcatgccatc cgtaagatgc 6480 ttttctgtga ctggtgagta
ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6540 agttgctctt
gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6600
gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
6660 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
ttttactttc 6720 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
ccgcaaaaaa gggaataagg 6780 gcgacacgga aatgttgaat actcatactc
ttcctttttc aatattattg aagcatttat 6840 cagggttatt gtctcatgag
cggatacata tttgaatgta tttagaaaaa taaacaaata 6900 ggggttccgc
gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg 6960
ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
7020 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
tgttccagtt 7080 tggaacaaga gtccactatt aaagaacgtg gactccaacg
tcaaagggcg aaaaaccgtc 7140 tatcagggcg atggcccact acgtgaacca
tcaccctaat caagtttttt ggggtcgagg 7200 tgccgtaaag cactaaatcg
gaaccctaaa gggagccccc gatttagagc ttgacgggga 7260 aagccggcga
acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 7320
ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
7380 ctacagggcg cgtcccattc gcca 7404 <210> SEQ ID NO 52
<211> LENGTH: 7404 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: Vector pFr7(DV) <400> SEQUENCE: 52 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gcaagcttct 180 agtcgcggta gaacgacccc atgtagcgga tgtactcgtc
ctcggtgtgg tttgccttcc 240
agtcgtggac ggagtccttg ttgcgctggc cggcgatgac ggagagctcc ttacccagct
300 tctcaaaggc ctcgtcaacg cactcctcgg gggtgagggc gatcttcatg
acggcctcgc 360 cctgcgggcc gccggggagg ttggacagca ggctgggggt
tagggtggtg ccgagggtga 420 tgacctcgac gtcgacgccg gtgccctcgc
actcgcaggc cacggcctcg gtcatcttga 480 ggatgaaggc cttgcccgcg
ccgtactggc cgttccaggg gctggagctg atgccggtca 540 tcgacgagac
gttgatcacg gcgccgcggt cctgggcggc aaagatccgc atgtagtggt 600
ggaagcactt gaggaaggtc acgacgttga cgttgatcat ggcctcgtgc ttctcccagg
660 gggtgtcctg gatcttaccg aagctgtgca ggcaggccac gtagctcatg
aagcccatgt 720 ccaggccctc ggtcgcggcg aagacggtct cggcagcgcc
gggctggcta aagtcggcgc 780 gcacgacctt ggtctccacg ccgtaggtct
cgcggatctc gcctgcgagc acgttcagct 840 tctcctcgcg aacgtcgacc
atgacgacgt tcatgccgcc ggcggcgatc ttctcgcaga 900 acgccttgcc
gacgccctcg gtcgcgccca ggatcaggcc ccactcaccg tacttctccc 960
tcaggttcat gtatatctcc tttcacgaat tctcagccgc cttcttgaac ttggcggcct
1020 cttccgaacc gccggtggca ttgcccttcg agtaggaatg cgcgccggtg
ccggcaagag 1080 cgccgccctg cacgatgagg tattcgtcgc ggatcggacg
gccctcgaag aagcactcca 1140 ggatctcgcg ggtgcccgcc gcataacgcg
cctgcgcggt cagcgtggtg ccggagatgt 1200 gcggggtcat gccgttatag
ggcatcgtcc gccaggggtg gtccttcggc gccggctgcg 1260 ggaaccacac
gtcgccggca tagccggcca gccggccgga ttcgagcgca cgtgccacgg 1320
catcgcggtc gcacagcttg ccgcgggcgg tgttgacgat gtaggcgcca cgcttgaaca
1380 gcttcagcgt ctcgtcattg atcatgtgct cggtttcggg gtgcagcggg
cagttcagcg 1440 tcaccacgtc gcaaaccgga tacatgtcct cgcgggtcgc
gtgccaggtg aggttgagct 1500 ccttctcgac cgattccggc aggcggtgac
ggccggtgta gtgcaggtgc acgtcgaacg 1560 gcgccagacg gcgcagcacc
gcgagaccga tgcggccggc ggccacggtg ccgacatgca 1620 tcgcctcgag
gtcgtaggcg tgggagacgc agtcggcgat gttccagccg cccttccgcg 1680
cccattcgtg cgagggcaga tagttgcgca ccagcgacag gatcatcatc accacatgct
1740 cggcgacgct gatcgagttg cagtaggtga cttccgccac ggtgacgttg
cggtcgatag 1800 ccgactgaag atcgacgtgg tcggaaccga tgccggcggt
gagcgcgagc ttcaggttct 1860 tggccttggc gatgcgctcg ggcgtcagat
aggccggcca gaagggctgg gagatgacga 1920 catccgcatc gaccagctcg
cgctcgaaca ccgagtcggg gccgtccttg tcggaggtca 1980 cgaccagggt
gtggccgttg gattcgagat attcgcgcag gccgagctcg ccggagacgg 2040
agccgagcaa ctgcccgggc gtgaagtcga tggccttcgg cgtcggcaag atctggccgc
2100 ccggatagtg gtcgatcttc ggaagatcgt cgcgggcata ggtcttcggg
tagccgtcga 2160 ccggatcatc gtaaagaacg cacaggacct ttgccatcat
atgtatatct ccttcttaaa 2220 gttaaacaaa attatttcta gaggggaatt
gttatccgct cacaattccc ctatagtgag 2280 tcgtattaat ttcgcgggat
cgagatctcg atcctctacg ccggacgcat cgtggccggc 2340 atcaccggcg
ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 2400
gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
2460 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
tgcggcggcg 2520 gtgctcaacg gcctcaacct actactgggc tgcttcctaa
tgcaggagtc gcataaggga 2580 gagcgtcgag atcccggaca ccatcgaatg
gcgcaaaacc tttcgcggta tggcatgata 2640 gcgcccggaa gagagtcaat
tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 2700 cgcagagtat
gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 2760
cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
2820 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
ttgccacctc 2880 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
attaaatctc gcgccgatca 2940 actgggtgcc agcgtggtgg tgtcgatggt
agaacgaagc ggcgtcgaag cctgtaaagc 3000 ggcggtgcac aatcttctcg
cgcaacgcgt cagtgggctg atcattaact atccgctgga 3060 tgaccaggat
gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 3120
tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
3180 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
cgggcccatt 3240 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
aaatatctca ctcgcaatca 3300 aattcagccg atagcggaac gggaaggcga
ctggagtgcc atgtccggtt ttcaacaaac 3360 catgcaaatg ctgaatgagg
gcatcgttcc cactgcgatg ctggttgcca acgatcagat 3420 ggcgctgggc
gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 3480
ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
3540 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
aactctctca 3600 gggccaggcg gtgaagggca atcagctgtt gcccgtctca
ctggtgaaaa gaaaaaccac 3660 cctggcgccc aatacgcaaa ccgcctctcc
ccgcgcgttg gccgattcat taatgcagct 3720 ggcacgacag gtttcccgac
tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 3780 agctcactca
ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 3840
gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
3900 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
gaggaccgct 3960 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
attcggaatc ttgcacgccc 4020 tcgctcaagc cttcgtcact ggtcccgcca
ccaaacgttt cggcgagaag caggccatta 4080 tcgccggcat ggcggcccca
cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 4140 taggctggcg
gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt 4200
gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
4260 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
atgttccgga 4320 tctgcatcgc aggatgctgc tggctaccct gtggaacacc
tacatctgta ttaacgaagc 4380 gctggcattg accctgagtg atttttctct
ggtcccgccg catccatacc gccagttgtt 4440 taccctcaca acgttccagt
aaccgggcat gttcatcatc agtaacccgt atcgtgagca 4500 tcctctctcg
tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg 4560
catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
4620 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
gacatctgtg 4680 aatcgcttca cgaccacgct gatgagcttt accgcagctg
cctcgcgcgt ttcggtgatg 4740 acggtgaaaa cctctgacac atgcagctcc
cggagacggt cacagcttgt ctgtaagcgg 4800 atgccgggag cagacaagcc
cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 4860 cagccatgac
ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 4920
agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta
4980 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
gctgcgctcg 5040 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
cggtaatacg gttatccaca 5100 gaatcagggg ataacgcagg aaagaacatg
tgagcaaaag gccagcaaaa ggccaggaac 5160 cgtaaaaagg ccgcgttgct
ggcgtttttc cataggctcc gcccccctga cgagcatcac 5220 aaaaatcgac
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 5280
tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
5340 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
ctgtaggtat 5400 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
tgcacgaacc ccccgttcag 5460 cccgaccgct gcgccttatc cggtaactat
cgtcttgagt ccaacccggt aagacacgac 5520 ttatcgccac tggcagcagc
cactggtaac aggattagca gagcgaggta tgtaggcggt 5580 gctacagagt
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 5640
atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
5700 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
tacgcgcaga 5760 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
ggtctgacgc tcagtggaac 5820 gaaaactcac gttaagggat tttggtcatg
agattatcaa aaaggatctt cacctagatc 5880 cttttaaatt aaaaatgaag
ttttaaatca atctaaagta tatatgagta aacttggtct 5940 gacagttacc
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 6000
tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
6060 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
tttatcagca 6120 ataaaccagc cagccggaag ggccgagcgc agaagtggtc
ctgcaacttt atccgcctcc 6180 atccagtcta ttaattgttg ccgggaagct
agagtaagta gttcgccagt taatagtttg 6240 cgcaacgttg ttgccattgc
tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6300 tcattcagct
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6360
aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
6420 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
cgtaagatgc 6480 ttttctgtga ctggtgagta ctcaaccaag tcattctgag
aatagtgtat gcggcgaccg 6540 agttgctctt gcccggcgtc aatacgggat
aataccgcgc cacatagcag aactttaaaa 6600 gtgctcatca ttggaaaacg
ttcttcgggg cgaaaactct caaggatctt accgctgttg 6660 agatccagtt
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6720
accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
6780 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
aagcatttat 6840 cagggttatt gtctcatgag cggatacata tttgaatgta
tttagaaaaa taaacaaata 6900 ggggttccgc gcacatttcc ccgaaaagtg
ccacctgaaa ttgtaaacgt taatattttg 6960 ttaaaattcg cgttaaattt
ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 7020 ggcaaaatcc
cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 7080
tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
7140 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
ggggtcgagg 7200 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
gatttagagc ttgacgggga 7260 aagccggcga acgtggcgag aaaggaaggg
aagaaagcga aaggagcggg cgctagggcg 7320 ctggcaagtg tagcggtcac
gctgcgcgta accaccacac ccgccgcgct taatgcgccg 7380 ctacagggcg
cgtcccattc gcca 7404 <210> SEQ ID NO 53 <211> LENGTH:
27 <212> TYPE: DNA <213> ORGANISM: artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
"G39D for" <400> SEQUENCE: 53 gtcgtcatgg tcgaccgtcg cgaggag
27
<210> SEQ ID NO 54 <211> LENGTH: 27 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer "G39D_rev" <400>
SEQUENCE: 54 ctcctcgcga cggtcgacca tgacgac 27 <210> SEQ ID NO
55 <211> LENGTH: 24 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer "G39D/R40I_for" <400> SEQUENCE: 55
gtcatggtcg acattcgcga ggag 24 <210> SEQ ID NO 56 <211>
LENGTH: 24 <212> TYPE: DNA <213> ORGANISM: artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
"G39D/R40I_rev" <400> SEQUENCE: 56 ctcctcgcga atgtcgacca tgac
24 <210> SEQ ID NO 57 <211> LENGTH: 27 <212>
TYPE: DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer "R40D_for" <400>
SEQUENCE: 57 gtcatggtcg gcgatcgcga ggagaag 27 <210> SEQ ID NO
58 <211> LENGTH: 27 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: PCR Primer "R40D_rev" <400> SEQUENCE: 58
cttctcctcg cgatcgccga ccatgac 27 <210> SEQ ID NO 59
<211> LENGTH: 30 <212> TYPE: DNA <213> ORGANISM:
artificial <220> FEATURE: <223> OTHER INFORMATION: PCR
Primer "R40D/R41I_for" <400> SEQUENCE: 59 gtcatggtcg
gcgatatcga ggagaagctg 30 <210> SEQ ID NO 60 <211>
LENGTH: 30 <212> TYPE: DNA <213> ORGANISM: artificial
<220> FEATURE: <223> OTHER INFORMATION: PCR Primer
"R40D/R41I_rev" <400> SEQUENCE: 60 cagcttctcc tcgatatcgc
cgaccatgac 30 <210> SEQ ID NO 61 <211> LENGTH: 27
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: PCR Primer "DIN_for"
<400> SEQUENCE: 61 atggtcgaca ttaacgagga gaagctg 27
<210> SEQ ID NO 62 <211> LENGTH: 27 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: PCR Primer "DIN_rev" <400>
SEQUENCE: 62 cagcttctcc tcgttaatgt cgaccat 27 <210> SEQ ID NO
63 <211> LENGTH: 8321 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: Plasmid pFr3T7(D) <400> SEQUENCE: 63 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gcctagtcgc 180 ggtagaacga ccccatgtag cggatgtact cgtcctcggt
gtggtttgcc ttccagtcgt 240 ggacggagtc cttgttgcgc tggccggcga
tgacggagag ctccttaccc agcttctcaa 300 aggcctcgtc aacgcactcc
tcgggggtga gggcgatctt catgacggcc tcgccctgcg 360 ggccgccggg
gaggttggac agcaggctgg gggttagggt ggtgccgagg gtgatgacct 420
cgacgtcgac gccggtgccc tcgcactcgc aggccacggc ctcggtcatc ttgaggatga
480 aggccttgcc cgcgccgtac tggccgttcc aggggctgga gctgatgccg
gtcatcgacg 540 agacgttgat cacggcgccg cggtcctggg cggcaaagat
ccgcatgtag tggtggaagc 600 acttgaggaa ggtcacgacg ttgacgttga
tcatggcctc gtgcttctcc cagggggtgt 660 cctggatctt accgaagctg
tgcaggcagg ccacgtagct catgaagccc atgtccaggc 720 cctcggtcgc
ggcgaagacg gtctcggcag cgccgggctg gctaaagtcg gcgcgcacga 780
ccttggtctc cacgccgtag gtctcgcgga tctcgcctgc gagcacgttc agcttctcct
840 cgcgacggtc gaccatgacg acgttcatgc cgccggcggc gatcttctcg
cagaacgcct 900 tgccgacgcc ctcggtcgcg cccaggatca ggccccactc
accgtacttc tccctcaggt 960 tcatatgtat atctccttct tatacttaac
taatatacta agatggggaa ttgttatccg 1020 ctcacaattc ccctatagtg
agtcgtatta atttcgatta tgcggccgtg tacaatacga 1080 ttactttctg
ttcgacttaa gcattataag ctttcagaac tgtgtcgggc gcatcaccgc 1140
atcaatgccg ccatcaatga cgatctgcgc gccatgcaca tagcttgcgg ccgggctcat
1200 caaaaaggcg atgaccgacg ccatctcgga cggctcggca cggcggccca
tgggaggaac 1260 gaacttggca atggattcgc catagcgcgg gtcctgcagg
cccgcctgca gcaagggagt 1320 ctcggttgca ccgggggcga tggtgttcag
gcgcacgcca gcctcgcccc aggcggcggc 1380 gcgtttgcgc acagccaccg
tcaaagcatt cttgctgccc gcataggcca gatttccgcc 1440 ctgctctccc
gcatgttcga caatggcgcg ggccttggct tcctcgccgg cttccagtgc 1500
cagcgccagt gggttcttgt caaaagccag atgcgcggaa gccacggacg agatgacgac
1560 ggctgcgggc tgatggcctt ttttcagcgc tggcaaaaag gcatccatca
gctcggtcgc 1620 gccaaaataa ttgaccgaaa ccacattgcc aagcaccttg
gtctgcggtc ccaggccggc 1680 gcacagcacc aggccgtcca tgcccttgct
gcacttcgcc agtacatcgg caatcgcctg 1740 ctttcgacct tcggccgtcg
agagatcggc aatcacttcc gcatcgcgta tatcgatgcc 1800 tacgatctgg
tgaccggccg cctccaggac cttgcgcgta gccgcaccaa tgccggtggc 1860
gcagccgctt atcacgatga tggacatgta tatctccttt cacgaattct cagccgcctt
1920 cttgaacttg gcggcctctt ccgaaccgcc ggtggcattg cccttcgagt
aggaatgcgc 1980 gccggtgccg gcaagagcgc cgccctgcac gatgaggtat
tcgtcgcgga tcggacggcc 2040 ctcgaagaag cactccagga tctcgcgggt
gcccgccgca taacgcgcct gcgcggtcag 2100 cgtggtgccg gagatgtgcg
gggtcatgcc gttatagggc atcgtccgcc aggggtggtc 2160 cttcggcgcc
ggctgcggga accacacgtc gccggcatag ccggccagcc ggccggattc 2220
gagcgcacgt gccacggcat cgcggtcgca cagcttgccg cgggcggtgt tgacgatgta
2280 ggcgccacgc ttgaacagct tcagcgtctc gtcattgatc atgtgctcgg
tttcggggtg 2340 cagcgggcag ttcagcgtca ccacgtcgca aaccggatac
atgtcctcgc gggtcgcgtg 2400 ccaggtgagg ttgagctcct tctcgaccga
ttccggcagg cggtgacggt cggtgtagtg 2460 caggtgcacg tcgaacggcg
ccagacggcg cagcaccgcg agaccgatgc ggccggcggc 2520 cacggtgccg
acatgcatcg cctcgaggtc gtaggcgtgg gagacgcagt cggcgatgtt 2580
ccagccgccc ttccgcgccc attcgtgcga gggcagatag ttgcgcacca gcgacaggat
2640 catcatcacc acatgctcgg cgacgctgat cgagttgcag taggtgactt
ccgccacggt 2700 gacgttgcgg tcgatagccg actgaagatc gacgtggtcg
gaaccgatgc cggcggtgag 2760 cgcgagcttc aggttcttgg ccttggcgat
gcgctcgggc gtcagatagg ccggccagaa 2820 gggctgggag atgacgacat
ccgcatcgac cagctcgcgc tcgaacaccg agtcggggcc 2880 gtccttgtcg
gaggtcacga ccagggtgtg gccgttggat tcgagatatt cgcgcaggcc 2940
gagctcgccg gagacggagc cgagcaactg cccgggcgtg aagtcgatgg ccttcggcgt
3000 cggcaagatc tggccgcccg gatagtggtc gatcttcgga agatcgtcgc
gggcataggt 3060 cttcgggtag ccgtcgaccg gatcatcgta aagaacgcac
aggacctttg ccatcatatg 3120 tatatctcct tcttaaagtt aaacaaaatt
atttctagag gggaattgtt atccgctcac 3180 aattccccta tagtgagtcg
tattaatttc gcgggatcga gatctcgatc ctctacgccg 3240 gacgcatcgt
ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 3300
acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
3360 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
ttgcatgcac 3420 cattccttgc ggcggcggtg ctcaacggcc tcaacctact
actgggctgc ttcctaatgc 3480 aggagtcgca taagggagag cgtcgagatc
ccggacacca tcgaatggcg caaaaccttt 3540 cgcggtatgg catgatagcg
cccggaagag agtcaattca gggtggtgaa tgtgaaacca 3600 gtaacgttat
acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 3660
gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
3720 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
gtcgttgctg 3780 attggcgttg ccacctccag tctggccctg cacgcgccgt
cgcaaattgt cgcggcgatt 3840 aaatctcgcg ccgatcaact gggtgccagc
gtggtggtgt cgatggtaga acgaagcggc 3900 gtcgaagcct gtaaagcggc
ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 3960
attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
4020 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
tttctcccat 4080 gaagacggta cgcgactggg cgtggagcat ctggtcgcat
tgggtcacca gcaaatcgcg 4140 ctgttagcgg gcccattaag ttctgtctcg
gcgcgtctgc gtctggctgg ctggcataaa 4200 tatctcactc gcaatcaaat
tcagccgata gcggaacggg aaggcgactg gagtgccatg 4260 tccggttttc
aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 4320
gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
4380 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
atgttatatc 4440 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
ggcaaaccag cgtggaccgc 4500 ttgctgcaac tctctcaggg ccaggcggtg
aagggcaatc agctgttgcc cgtctcactg 4560 gtgaaaagaa aaaccaccct
ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 4620 gattcattaa
tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 4680
cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
4740 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
tcgccgcact 4800 tatgactgtc ttctttatca tgcaactcgt aggacaggtg
ccggcagcgc tctgggtcat 4860 tttcggcgag gaccgctttc gctggagcgc
gacgatgatc ggcctgtcgc ttgcggtatt 4920 cggaatcttg cacgccctcg
ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 4980 cgagaagcag
gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 5040
gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
5100 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
gagcaacaac 5160 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
acgcggaagt cagcgccctg 5220 caccattatg ttccggatct gcatcgcagg
atgctgctgg ctaccctgtg gaacacctac 5280 atctgtatta acgaagcgct
ggcattgacc ctgagtgatt tttctctggt cccgccgcat 5340 ccataccgcc
agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 5400
aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
5460 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
acatggcccg 5520 ctttatcaga agccagacat taacgcttct ggagaaactc
aacgagctgg acgcggatga 5580 acaggcagac atctgtgaat cgcttcacga
ccacgctgat gagctttacc gcagctgcct 5640 cgcgcgtttc ggtgatgacg
gtgaaaacct ctgacacatg cagctcccgg agacggtcac 5700 agcttgtctg
taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 5760
tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
5820 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg
cggtgtgaaa 5880 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
ctcttccgct tcctcgctca 5940 ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg 6000 taatacggtt atccacagaa
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6060 agcaaaaggc
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6120
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
6180 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc 6240 tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg ctttctcata 6300 gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc 6360 acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6420 acccggtaag
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6480
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
6540 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
aaaagagttg 6600 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
tggttttttt gtttgcaagc 6660 agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt 6720 ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6780 ggatcttcac
ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6840
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
6900 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
actacgatac 6960 gggagggctt accatctggc cccagtgctg caatgatacc
gcgagaccca cgctcaccgg 7020 ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg 7080 caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt 7140 cgccagttaa
tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 7200
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7260 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta 7320 agttggccgc agtgttatca ctcatggtta tggcagcact
gcataattct cttactgtca 7380 tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat 7440 agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat acgggataat accgcgccac 7500 atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 7560
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
7620 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg 7680 caaaaaaggg aataagggcg acacggaaat gttgaatact
catactcttc ctttttcaat 7740 attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt 7800 agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgaaattg 7860 taaacgttaa
tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 7920
accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
7980 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
tccaacgtca 8040 aagggcgaaa aaccgtctat cagggcgatg gcccactacg
tgaaccatca ccctaatcaa 8100 gttttttggg gtcgaggtgc cgtaaagcac
taaatcggaa ccctaaaggg agcccccgat 8160 ttagagcttg acggggaaag
ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 8220 gagcgggcgc
tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 8280
ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a 8321 <210> SEQ
ID NO 64 <211> LENGTH: 8190 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: pF(G)r7(A)r3 <400> SEQUENCE: 64 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gctcagaact 180 gtgtcgggcg catcaccgca tcaatgccgc catcaatgac
gatctgcgcg ccatgcacat 240 agcttgcggc cgggctcatc aaaaaggcga
tgaccgacgc catctcggac ggctcggcac 300 ggcggcccat gggaggaacg
aacttggcaa tggattcgcc atagcgcggg tcctgcaggc 360 ccgcctgcag
caagggagtc tcggttgcac cgggggcgat ggtgttcagg cgcacgccag 420
cctcgcccca ggcggcggcg cgtttgcgca cagccaccgt caaagcattc ttgctgcccg
480 cataggccag atttccgccc tgctctcccg catgttcgac aatggcgcgg
gccttggctt 540 cctcgccggc ttccagtgcc agcgccagtg ggttcttgtc
aaaagccaga tgcgcggaag 600 ccacggacga gatgacgacg gctgcgggct
gatggccttt tttcagcgct ggcaaaaagg 660 catccatcag ctcggtcgcg
ccaaaataat tgaccgaaac cacattgcca agcaccttgg 720 tctgcggtcc
caggccggcg cacagcacca ggccgtccat gcccttgctg cacttcgcca 780
gtacatcggc aatcgcctgc tttcgacctt cggccgtcga gagatcggca atcacttccg
840 catcgcgtat atcgatgcct acgatctggt gaccggccgc ctccaggacc
ttgcgcgtag 900 cggcaccaat gccggtggcg cagccgctta tcacgatgat
ggacatgtat atctccttaa 960 gcttctagtc gcggtagaac gaccccatgt
agcggatgta ctcgtcctcg gtgtggtttg 1020 ccttccagtc gtggacggag
tccttgttgc gctggccggc gatgacggag agctccttac 1080 ccagcttctc
aaaggcctcg tcaacgcact cctcgggggt gagggcgatc ttcatgacgg 1140
cctcgccctg cgggccgccg gggaggttgg acagcaggct gggggttagg gtggtgccga
1200 gggtgatgac ctcgacgtcg acgccggtgc cctcgcactc gcaggccacg
gcctcggtca 1260 tcttgaggat gaaggccttg cccgcgccgt actggccgtt
ccaggggctg gagctgatgc 1320 cggtcatcga cgagacgttg atcacggcgc
cgcggtcctg ggcggcaaag atccgcatgt 1380 agtggtggaa gcacttgagg
aaggtcacga cgttgacgtt gatcatggcc tcgtgcttct 1440 cccagggggt
gtcctggatc ttaccgaagc tgtgcaggca ggccacgtag ctcatgaagc 1500
ccatgtccag gccctcggtc gcggcgaaga cggtctcggc agcgccgggc tggctaaagt
1560 cggcgcgcac gaccttggtc tccacgccgt aggtctcgcg gatctcgcct
gcgagcacgt 1620 tcagcttctc ctcgcgacgg gcgaccatga cgacgttcat
gccgccggcg gcgatcttct 1680 cgcagaacgc cttgccgacg ccctcggtcg
cgcccaggat caggccccac tcaccgtact 1740 tctccctcag gttcatgtat
atctcctttc acgaattctc agccgccttc ttgaacttgg 1800 cggcctcttc
cgaaccgccg gtggcattgc ccttcgagta ggaatgcgcg ccggtgccgg 1860
caagagcgcc gccctgcacg atgaggtatt cgtcgcggat cggacggccc tcgaagaagc
1920 actccaggat ctcgcgggtg cccgccgcat aacgcgcctg cgcggtcagc
gtggtgccgg 1980 agatgtgcgg ggtcatgccg ttatagggca tcgtccgcca
ggggtggtcc ttcggcgccg 2040 gctgcgggaa ccacacgtcg ccggcatagc
cggccagccg gccggattcg agcgcacgtg 2100 ccacggcatc gcggtcgcac
agcttgccgc gggcggtgtt gacgatgtag gcgccacgct 2160 tgaacagctt
cagcgtctcg tcattgatca tgtgctcggt ttcggggtgc agcgggcagt 2220
tcagcgtcac cacgtcgcaa accggataca tgtcctcgcg ggtcgcgtgc caggtgaggt
2280 tgagctcctt ctcgaccgat tccggcaggc ggtgacggcc ggtgtagtgc
aggtgcacgt 2340 cgaacggcgc cagacggcgc agcaccgcga gaccgatgcg
gccggcggcc acggtgccga 2400 catgcatcgc ctcgaggtcg taggcgtggg
agacgcagtc ggcgatgttc cagccgccct 2460 tccgcgccca ttcgtgcgag
ggcagatagt tgcgcaccag cgacaggatc atcatcacca 2520 catgctcggc
gacgctgatc gagttgcagt aggtgacttc cgccacggtg acgttgcggt 2580
cgatagccga ctgaagatcg acgtggtcgg aaccgatgcc ggcggtgagc gcgagcttca
2640 ggttcttggc cttggcgatg cgctcgggcg tcagataggc cggccagaag
ggctgggaga 2700 tgacgacatc cgcatcgacc agctcgcgct cgaacaccga
gtcggggccg tccttgtcgg 2760 aggtcacgac cagggtgtgg ccgttggatt
cgagatattc gcgcaggccg agctcgccgg 2820 agacggagcc gagcaactgc
ccgggcgtga agtcgatggc cttcggcgtc ggcaagatct 2880
ggccgcccgg atagtggtcg atcttcggaa gatcgtcgcg ggcataggtc ttcgggtagc
2940 cgtcgaccgg atcatcgtaa agaacgcaca ggacctttgc catcatatgt
atatctcctt 3000 cttaaagtta aacaaaatta tttctagagg ggaattgtta
tccgctcaca attcccctat 3060 agtgagtcgt attaatttcg cgggatcgag
atctcgatcc tctacgccgg acgcatcgtg 3120 gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat 3180 ggggaagatc
gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg 3240
gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
3300 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
ggagtcgcat 3360 aagggagagc gtcgagatcc cggacaccat cgaatggcgc
aaaacctttc gcggtatggc 3420 atgatagcgc ccggaagaga gtcaattcag
ggtggtgaat gtgaaaccag taacgttata 3480 cgatgtcgca gagtatgccg
gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 3540 cagccacgtt
tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 3600
cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
3660 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
aatctcgcgc 3720 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
cgaagcggcg tcgaagcctg 3780 taaagcggcg gtgcacaatc ttctcgcgca
acgcgtcagt gggctgatca ttaactatcc 3840 gctggatgac caggatgcca
ttgctgtgga agctgcctgc actaatgttc cggcgttatt 3900 tcttgatgtc
tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 3960
gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
4020 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
atctcactcg 4080 caatcaaatt cagccgatag cggaacggga aggcgactgg
agtgccatgt ccggttttca 4140 acaaaccatg caaatgctga atgagggcat
cgttcccact gcgatgctgg ttgccaacga 4200 tcagatggcg ctgggcgcaa
tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 4260 tatctcggta
gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 4320
caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
4380 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
tgaaaagaaa 4440 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
gcgttggccg attcattaat 4500 gcagctggca cgacaggttt cccgactgga
aagcgggcag tgagcgcaac gcaattaatg 4560 taagttagct cactcattag
gcaccgggat ctcgaccgat gcccttgaga gccttcaacc 4620 cagtcagctc
cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct 4680
tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
4740 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
ggaatcttgc 4800 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg 4860 ccattatcgc cggcatggcg gccccacggg
tgcgcatgat cgtgctcctg tcgttgagga 4920 cccggctagg ctggcggggt
tgccttactg gttagcagaa tgaatcaccg atacgcgagc 4980 gaacgtgaag
cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct 5040
tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
5100 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
tctgtattaa 5160 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
ccgccgcatc cataccgcca 5220 gttgtttacc ctcacaacgt tccagtaacc
gggcatgttc atcatcagta acccgtatcg 5280 tgagcatcct ctctcgtttc
atcggtatca ttacccccat gaacagaaat cccccttaca 5340 cggaggcatc
agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa 5400
gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
5460 tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
gcgcgtttcg 5520 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
gacggtcaca gcttgtctgt 5580 aagcggatgc cgggagcaga caagcccgtc
agggcgcgtc agcgggtgtt ggcgggtgtc 5640 ggggcgcagc catgacccag
tcacgtagcg atagcggagt gtatactggc ttaactatgc 5700 ggcatcagag
cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga 5760
tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
5820 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
aatacggtta 5880 tccacagaat caggggataa cgcaggaaag aacatgtgag
caaaaggcca gcaaaaggcc 5940 aggaaccgta aaaaggccgc gttgctggcg
tttttccata ggctccgccc ccctgacgag 6000 catcacaaaa atcgacgctc
aagtcagagg tggcgaaacc cgacaggact ataaagatac 6060 caggcgtttc
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6120
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
6180 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
cgaacccccc 6240 gttcagcccg accgctgcgc cttatccggt aactatcgtc
ttgagtccaa cccggtaaga 6300 cacgacttat cgccactggc agcagccact
ggtaacagga ttagcagagc gaggtatgta 6360 ggcggtgcta cagagttctt
gaagtggtgg cctaactacg gctacactag aaggacagta 6420 tttggtatct
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6480
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
6540 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
tgacgctcag 6600 tggaacgaaa actcacgtta agggattttg gtcatgagat
tatcaaaaag gatcttcacc 6660 tagatccttt taaattaaaa atgaagtttt
aaatcaatct aaagtatata tgagtaaact 6720 tggtctgaca gttaccaatg
cttaatcagt gaggcaccta tctcagcgat ctgtctattt 6780 cgttcatcca
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 6840
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
6900 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
aactttatcc 6960 gcctccatcc agtctattaa ttgttgccgg gaagctagag
taagtagttc gccagttaat 7020 agtttgcgca acgttgttgc cattgctgca
ggcatcgtgg tgtcacgctc gtcgtttggt 7080 atggcttcat tcagctccgg
ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 7140 tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 7200
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
7260 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
gtgtatgcgg 7320 cgaccgagtt gctcttgccc ggcgtcaata cgggataata
ccgcgccaca tagcagaact 7380 ttaaaagtgc tcatcattgg aaaacgttct
tcggggcgaa aactctcaag gatcttaccg 7440 ctgttgagat ccagttcgat
gtaacccact cgtgcaccca actgatcttc agcatctttt 7500 actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 7560
ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
7620 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
gaaaaataaa 7680 caaatagggg ttccgcgcac atttccccga aaagtgccac
ctgaaattgt aaacgttaat 7740 attttgttaa aattcgcgtt aaatttttgt
taaatcagct cattttttaa ccaataggcc 7800 gaaatcggca aaatccctta
taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 7860 ccagtttgga
acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 7920
accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
7980 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
tagagcttga 8040 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
aagcgaaagg agcgggcgct 8100 agggcgctgg caagtgtagc ggtcacgctg
cgcgtaacca ccacacccgc cgcgcttaat 8160 gcgccgctac agggcgcgtc
ccattcgcca 8190 <210> SEQ ID NO 65 <211> LENGTH: 8190
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: pF(G)r7(S)r3 <400>
SEQUENCE: 65 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagtgcggcc gctcagaact 180 gtgtcgggcg catcaccgca
tcaatgccgc catcaatgac gatctgcgcg ccatgcacat 240 agcttgcggc
cgggctcatc aaaaaggcga tgaccgacgc catctcggac ggctcggcac 300
ggcggcccat gggaggaacg aacttggcaa tggattcgcc atagcgcggg tcctgcaggc
360 ccgcctgcag caagggagtc tcggttgcac cgggggcgat ggtgttcagg
cgcacgccag 420 cctcgcccca ggcggcggcg cgtttgcgca cagccaccgt
caaagcattc ttgctgcccg 480 cataggccag atttccgccc tgctctcccg
catgttcgac aatggcgcgg gccttggctt 540 cctcgccggc ttccagtgcc
agcgccagtg ggttcttgtc aaaagccaga tgcgcggaag 600 ccacggacga
gatgacgacg gctgcgggct gatggccttt tttcagcgct ggcaaaaagg 660
catccatcag ctcggtcgcg ccaaaataat tgaccgaaac cacattgcca agcaccttgg
720 tctgcggtcc caggccggcg cacagcacca ggccgtccat gcccttgctg
cacttcgcca 780 gtacatcggc aatcgcctgc tttcgacctt cggccgtcga
gagatcggca atcacttccg 840 catcgcgtat atcgatgcct acgatctggt
gaccggccgc ctccaggacc ttgcgcgtag 900 cggcaccaat gccggtggcg
cagccgctta tcacgatgat ggacatgtat atctccttaa 960 gcttctagtc
gcggtagaac gaccccatgt agcggatgta ctcgtcctcg gtgtggtttg 1020
ccttccagtc gtggacggag tccttgttgc gctggccggc gatgacggag agctccttac
1080 ccagcttctc aaaggcctcg tcaacgcact cctcgggggt gagggcgatc
ttcatgacgg 1140 cctcgccctg cgggccgccg gggaggttgg acagcaggct
gggggttagg gtggtgccga 1200 gggtgatgac ctcgacgtcg acgccggtgc
cctcgcactc gcaggccacg gcctcggtca 1260 tcttgaggat gaaggccttg
cccgcgccgt actggccgtt ccaggggctg gagctgatgc 1320 cggtcatcga
cgagacgttg atcacggcgc cgcggtcctg ggcggcaaag atccgcatgt 1380
agtggtggaa gcacttgagg aaggtcacga cgttgacgtt gatcatggcc tcgtgcttct
1440 cccagggggt gtcctggatc ttaccgaagc tgtgcaggca ggccacgtag
ctcatgaagc 1500 ccatgtccag gccctcggtc gcggcgaaga cggtctcggc
agcgccgggc tggctaaagt 1560 cggcgcgcac gaccttggtc tccacgccgt
aggtctcgcg gatctcgcct gcgagcacgt 1620 tcagcttctc ctcgcgacgg
ctgaccatga cgacgttcat gccgccggcg gcgatcttct 1680 cgcagaacgc
cttgccgacg ccctcggtcg cgcccaggat caggccccac tcaccgtact 1740
tctccctcag gttcatgtat atctcctttc acgaattctc agccgccttc ttgaacttgg
1800 cggcctcttc cgaaccgccg gtggcattgc ccttcgagta ggaatgcgcg
ccggtgccgg 1860
caagagcgcc gccctgcacg atgaggtatt cgtcgcggat cggacggccc tcgaagaagc
1920 actccaggat ctcgcgggtg cccgccgcat aacgcgcctg cgcggtcagc
gtggtgccgg 1980 agatgtgcgg ggtcatgccg ttatagggca tcgtccgcca
ggggtggtcc ttcggcgccg 2040 gctgcgggaa ccacacgtcg ccggcatagc
cggccagccg gccggattcg agcgcacgtg 2100 ccacggcatc gcggtcgcac
agcttgccgc gggcggtgtt gacgatgtag gcgccacgct 2160 tgaacagctt
cagcgtctcg tcattgatca tgtgctcggt ttcggggtgc agcgggcagt 2220
tcagcgtcac cacgtcgcaa accggataca tgtcctcgcg ggtcgcgtgc caggtgaggt
2280 tgagctcctt ctcgaccgat tccggcaggc ggtgacggcc ggtgtagtgc
aggtgcacgt 2340 cgaacggcgc cagacggcgc agcaccgcga gaccgatgcg
gccggcggcc acggtgccga 2400 catgcatcgc ctcgaggtcg taggcgtggg
agacgcagtc ggcgatgttc cagccgccct 2460 tccgcgccca ttcgtgcgag
ggcagatagt tgcgcaccag cgacaggatc atcatcacca 2520 catgctcggc
gacgctgatc gagttgcagt aggtgacttc cgccacggtg acgttgcggt 2580
cgatagccga ctgaagatcg acgtggtcgg aaccgatgcc ggcggtgagc gcgagcttca
2640 ggttcttggc cttggcgatg cgctcgggcg tcagataggc cggccagaag
ggctgggaga 2700 tgacgacatc cgcatcgacc agctcgcgct cgaacaccga
gtcggggccg tccttgtcgg 2760 aggtcacgac cagggtgtgg ccgttggatt
cgagatattc gcgcaggccg agctcgccgg 2820 agacggagcc gagcaactgc
ccgggcgtga agtcgatggc cttcggcgtc ggcaagatct 2880 ggccgcccgg
atagtggtcg atcttcggaa gatcgtcgcg ggcataggtc ttcgggtagc 2940
cgtcgaccgg atcatcgtaa agaacgcaca ggacctttgc catcatatgt atatctcctt
3000 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
attcccctat 3060 agtgagtcgt attaatttcg cgggatcgag atctcgatcc
tctacgccgg acgcatcgtg 3120 gccggcatca ccggcgccac aggtgcggtt
gctggcgcct atatcgccga catcaccgat 3180 ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg 3240 gcaggccccg
tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg 3300
gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
3360 aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
gcggtatggc 3420 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
gtgaaaccag taacgttata 3480 cgatgtcgca gagtatgccg gtgtctctta
tcagaccgtt tcccgcgtgg tgaaccaggc 3540 cagccacgtt tctgcgaaaa
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 3600 cattcccaac
cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 3660
cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
3720 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
tcgaagcctg 3780 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
gggctgatca ttaactatcc 3840 gctggatgac caggatgcca ttgctgtgga
agctgcctgc actaatgttc cggcgttatt 3900 tcttgatgtc tctgaccaga
cacccatcaa cagtattatt ttctcccatg aagacggtac 3960 gcgactgggc
gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 4020
cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
4080 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
ccggttttca 4140 acaaaccatg caaatgctga atgagggcat cgttcccact
gcgatgctgg ttgccaacga 4200 tcagatggcg ctgggcgcaa tgcgcgccat
taccgagtcc gggctgcgcg ttggtgcgga 4260 tatctcggta gtgggatacg
acgataccga agacagctca tgttatatcc cgccgttaac 4320 caccatcaaa
caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 4380
ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
4440 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
attcattaat 4500 gcagctggca cgacaggttt cccgactgga aagcgggcag
tgagcgcaac gcaattaatg 4560 taagttagct cactcattag gcaccgggat
ctcgaccgat gcccttgaga gccttcaacc 4620 cagtcagctc cttccggtgg
gcgcggggca tgactatcgt cgccgcactt atgactgtct 4680 tctttatcat
gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg 4740
accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
4800 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
gagaagcagg 4860 ccattatcgc cggcatggcg gccccacggg tgcgcatgat
cgtgctcctg tcgttgagga 4920 cccggctagg ctggcggggt tgccttactg
gttagcagaa tgaatcaccg atacgcgagc 4980 gaacgtgaag cgactgctgc
tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct 5040 tcggtttccg
tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt 5100
tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
5160 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
cataccgcca 5220 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
atcatcagta acccgtatcg 5280 tgagcatcct ctctcgtttc atcggtatca
ttacccccat gaacagaaat cccccttaca 5340 cggaggcatc agtgaccaaa
caggaaaaaa ccgcccttaa catggcccgc tttatcagaa 5400 gccagacatt
aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca 5460
tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg
5520 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
gcttgtctgt 5580 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
agcgggtgtt ggcgggtgtc 5640 ggggcgcagc catgacccag tcacgtagcg
atagcggagt gtatactggc ttaactatgc 5700 ggcatcagag cagattgtac
tgagagtgca ccatatatgc ggtgtgaaat accgcacaga 5760 tgcgtaagga
gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 5820
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
5880 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
gcaaaaggcc 5940 aggaaccgta aaaaggccgc gttgctggcg tttttccata
ggctccgccc ccctgacgag 6000 catcacaaaa atcgacgctc aagtcagagg
tggcgaaacc cgacaggact ataaagatac 6060 caggcgtttc cccctggaag
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6120 ggatacctgt
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6180
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
6240 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
cccggtaaga 6300 cacgacttat cgccactggc agcagccact ggtaacagga
ttagcagagc gaggtatgta 6360 ggcggtgcta cagagttctt gaagtggtgg
cctaactacg gctacactag aaggacagta 6420 tttggtatct gcgctctgct
gaagccagtt accttcggaa aaagagttgg tagctcttga 6480 tccggcaaac
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 6540
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
6600 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
gatcttcacc 6660 tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata tgagtaaact 6720 tggtctgaca gttaccaatg cttaatcagt
gaggcaccta tctcagcgat ctgtctattt 6780 cgttcatcca tagttgcctg
actccccgtc gtgtagataa ctacgatacg ggagggctta 6840 ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 6900
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
6960 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
gccagttaat 7020 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
tgtcacgctc gtcgtttggt 7080 atggcttcat tcagctccgg ttcccaacga
tcaaggcgag ttacatgatc ccccatgttg 7140 tgcaaaaaag cggttagctc
cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 7200 gtgttatcac
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 7260
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
7320 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
tagcagaact 7380 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag gatcttaccg 7440 ctgttgagat ccagttcgat gtaacccact
cgtgcaccca actgatcttc agcatctttt 7500 actttcacca gcgtttctgg
gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 7560 ataagggcga
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 7620
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
7680 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt
aaacgttaat 7740 attttgttaa aattcgcgtt aaatttttgt taaatcagct
cattttttaa ccaataggcc 7800 gaaatcggca aaatccctta taaatcaaaa
gaatagaccg agatagggtt gagtgttgtt 7860 ccagtttgga acaagagtcc
actattaaag aacgtggact ccaacgtcaa agggcgaaaa 7920 accgtctatc
agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 7980
tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
8040 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
agcgggcgct 8100 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
ccacacccgc cgcgcttaat 8160 gcgccgctac agggcgcgtc ccattcgcca 8190
<210> SEQ ID NO 66 <211> LENGTH: 7910 <212> TYPE:
DNA <213> ORGANISM: artificial <220> FEATURE:
<223> OTHER INFORMATION: p3T7(A)rG <400> SEQUENCE: 66
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc
gagttaaccg cggcctgcct 180 ggaatgaagg atattgtgtc ataccgccgt
ccgcgaataa cgtgatgcct gtgacgtagc 240 tggcttcctt cgaagcaagc
caggctgcta ctgcggcgat ctcctccggt tcgccgatat 300 atcccattgg
aatcatgctt tctacatcag ctttctgttt agggtcagcg aatttttcag 360
cattgattgg cgtgttgatc gcacctggcc caatattatt gacgcgaatg cccttcggcg
420 cgtattccaa cgctaatgtt tctgtcatca gctttatccc gcctttactt
gccgcatagt 480 ggacaaataa cggccaagga atcacttcgt gcacactgga
catgttaatg acatttccct 540 tgatatcgtt ttctacgaaa tatttaatcg
cttcacggct tcctaaaaag gcacccgtta 600 agttcgtgcc gatgacttta
tcccaatcct tgagcggcat ttcgtgagat ggcacaggat 660 tttcaagacc
ggcattatta atcataatat cgagtgtgcc gaactcctta attgccgttt 720
gcacgatatt ttttacatct tcctctttcg tgacatctcc ttggacgaca acagcttcac
780 cgcccgcctt gatgacctct tcttttacct cgttcggatc ttgtttatta
ctataatagt 840 tgataaccac ttttgcctgc tccttgccga agcgaatggc
catcgccttt ccgagccctg 900
aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacatg tatatctcct
960 tgcggccgcc tagtcgcggt agaacgaccc catgtagcgg atgtactcgt
cctcggtgtg 1020 gtttgccttc cagtcgtgga cggagtcctt gttgcgctgg
ccggcgatga cggagagctc 1080 cttacccagc ttctcaaagg cctcgtcaac
gcactcctcg ggggtgaggg cgatcttcat 1140 gacggcctcg ccctgcgggc
cgccggggag gttggacagc aggctggggg ttagggtggt 1200 gccgagggtg
atgacctcga cgtcgacgcc ggtgccctcg cactcgcagg ccacggcctc 1260
ggtcatcttg aggatgaagg ccttgcccgc gccgtactgg ccgttccagg ggctggagct
1320 gatgccggtc atcgacgaga cgttgatcac ggcgccgcgg tcctgggcgg
caaagatccg 1380 catgtagtgg tggaagcact tgaggaaggt cacgacgttg
acgttgatca tggcctcgtg 1440 cttctcccag ggggtgtcct ggatcttacc
gaagctgtgc aggcaggcca cgtagctcat 1500 gaagcccatg tccaggccct
cggtcgcggc gaagacggtc tcggcagcgc cgggctggct 1560 aaagtcggcg
cgcacgacct tggtctccac gccgtaggtc tcgcggatct cgcctgcgag 1620
cacgttcagc ttctcctcgc gacgggcgac catgacgacg ttcatgccgc cggcggcgat
1680 cttctcgcag aacgccttgc cgacgccctc ggtcgcgccc aggatcaggc
cccactcacc 1740 gtacttctcc ctcaggttca tatgtatatc tccttcttat
acttaactaa tatactaaga 1800 tggggaattg ttatccgctc acaattcccc
tatagtgagt cgtattaatt tcgattatgc 1860 ggccgtgtac aatacgatta
ctttctgttc gacttaagca ttataagctt gtcgacggag 1920 ctcgaattct
cagaactgtg tcgggcgcat caccgcatca atgccgccat caatgacgat 1980
ctgcgcgcca tgcacatagc ttgcggccgg gctcatcaaa aaggcgatga ccgacgccat
2040 ctcggacggc tcggcacggc ggcccatggg aggaacgaac ttggcaatgg
attcgccata 2100 gcgcgggtcc tgcaggcccg cctgcagcaa gggagtctcg
gttgcaccgg gggcgatggt 2160 gttcaggcgc acgccagcct cgccccaggc
ggcggcgcgt ttgcgcacag ccaccgtcaa 2220 agcattcttg ctgcccgcat
aggccagatt tccgccctgc tctcccgcat gttcgacaat 2280 ggcgcgggcc
ttggcttcct cgccggcttc cagtgccagc gccagtgggt tcttgtcaaa 2340
agccagatgc gcggaagcca cggacgagat gacgacggct gcgggctgat ggcctttttt
2400 cagcgctggc aaaaaggcat ccatcagctc ggtcgcgcca aaataattga
ccgaaaccac 2460 attgccaagc accttggtct gcggtcccag gccggcgcac
agcaccaggc cgtccatgcc 2520 cttgctgcac ttcgccagta catcggcaat
cgcctgcttt cgaccttcgg ccgtcgagag 2580 atcggcaatc acttccgcat
cgcgtatatc gatgcctacg atctggtgac cggccgcctc 2640 caggaccttg
cgcgtagccg caccaatgcc ggtggcgcag ccgcttatca cgatgatgga 2700
catcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
2760 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
atctcgatcc 2820 tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct 2880 atatcgccga catcaccgat ggggaagatc
gggctcgcca cttcgggctc atgagcgctt 2940 gtttcggcgt gggtatggtg
gcaggccccg tggccggggg actgttgggc gccatctcct 3000 tgcatgcacc
attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 3060
tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
3120 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
ggtggtgaat 3180 gtgaaaccag taacgttata cgatgtcgca gagtatgccg
gtgtctctta tcagaccgtt 3240 tcccgcgtgg tgaaccaggc cagccacgtt
tctgcgaaaa cgcgggaaaa agtggaagcg 3300 gcgatggcgg agctgaatta
cattcccaac cgcgtggcac aacaactggc gggcaaacag 3360 tcgttgctga
ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 3420
gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
3480 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
acgcgtcagt 3540 gggctgatca ttaactatcc gctggatgac caggatgcca
ttgctgtgga agctgcctgc 3600 actaatgttc cggcgttatt tcttgatgtc
tctgaccaga cacccatcaa cagtattatt 3660 ttctcccatg aagacggtac
gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 3720 caaatcgcgc
tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 3780
tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
3840 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
cgttcccact 3900 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
tgcgcgccat taccgagtcc 3960 gggctgcgcg ttggtgcgga tatctcggta
gtgggatacg acgataccga agacagctca 4020 tgttatatcc cgccgttaac
caccatcaaa caggattttc gcctgctggg gcaaaccagc 4080 gtggaccgct
tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 4140
gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
4200 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
aagcgggcag 4260 tgagcgcaac gcaattaatg taagttagct cactcattag
gcaccgggat ctcgaccgat 4320 gcccttgaga gccttcaacc cagtcagctc
cttccggtgg gcgcggggca tgactatcgt 4380 cgccgcactt atgactgtct
tctttatcat gcaactcgta ggacaggtgc cggcagcgct 4440 ctgggtcatt
ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 4500
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
4560 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg
tgcgcatgat 4620 cgtgctcctg tcgttgagga cccggctagg ctggcggggt
tgccttactg gttagcagaa 4680 tgaatcaccg atacgcgagc gaacgtgaag
cgactgctgc tgcaaaacgt ctgcgacctg 4740 agcaacaaca tgaatggtct
tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc 4800 agcgccctgc
accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 4860
aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
4920 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
gggcatgttc 4980 atcatcagta acccgtatcg tgagcatcct ctctcgtttc
atcggtatca ttacccccat 5040 gaacagaaat cccccttaca cggaggcatc
agtgaccaaa caggaaaaaa ccgcccttaa 5100 catggcccgc tttatcagaa
gccagacatt aacgcttctg gagaaactca acgagctgga 5160 cgcggatgaa
caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 5220
cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
5280 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
agggcgcgtc 5340 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
tcacgtagcg atagcggagt 5400 gtatactggc ttaactatgc ggcatcagag
cagattgtac tgagagtgca ccatatatgc 5460 ggtgtgaaat accgcacaga
tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 5520 cctcgctcac
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5580
caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
5640 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
tttttccata 5700 ggctccgccc ccctgacgag catcacaaaa atcgacgctc
aagtcagagg tggcgaaacc 5760 cgacaggact ataaagatac caggcgtttc
cccctggaag ctccctcgtg cgctctcctg 5820 ttccgaccct gccgcttacc
ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5880 tttctcatag
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5940
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
6000 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
ggtaacagga 6060 ttagcagagc gaggtatgta ggcggtgcta cagagttctt
gaagtggtgg cctaactacg 6120 gctacactag aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa 6180 aaagagttgg tagctcttga
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6240 tttgcaagca
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6300
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
6360 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
aaatcaatct 6420 aaagtatata tgagtaaact tggtctgaca gttaccaatg
cttaatcagt gaggcaccta 6480 tctcagcgat ctgtctattt cgttcatcca
tagttgcctg actccccgtc gtgtagataa 6540 ctacgatacg ggagggctta
ccatctggcc ccagtgctgc aatgataccg cgagacccac 6600 gctcaccggc
tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6660
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
6720 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
ggcatcgtgg 6780 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
ttcccaacga tcaaggcgag 6840 ttacatgatc ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg 6900 tcagaagtaa gttggccgca
gtgttatcac tcatggttat ggcagcactg cataattctc 6960 ttactgtcat
gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7020
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
7080 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
tcggggcgaa 7140 aactctcaag gatcttaccg ctgttgagat ccagttcgat
gtaacccact cgtgcaccca 7200 actgatcttc agcatctttt actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc 7260 aaaatgccgc aaaaaaggga
ataagggcga cacggaaatg ttgaatactc atactcttcc 7320 tttttcaata
ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 7380
aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
7440 ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
taaatcagct 7500 cattttttaa ccaataggcc gaaatcggca aaatccctta
taaatcaaaa gaatagaccg 7560 agatagggtt gagtgttgtt ccagtttgga
acaagagtcc actattaaag aacgtggact 7620 ccaacgtcaa agggcgaaaa
accgtctatc agggcgatgg cccactacgt gaaccatcac 7680 cctaatcaag
ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 7740
gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
7800 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
cgcgtaacca 7860 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
ccattcgcca 7910 <210> SEQ ID NO 67 <211> LENGTH: 7891
<212> TYPE: DNA <213> ORGANISM: artificial <220>
FEATURE: <223> OTHER INFORMATION: p7(A)T3rG <400>
SEQUENCE: 67 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa
ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt
ggtggtgctc gagttaaccg cggcctgcct 180
ggaatgaagg atattgtgtc ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc
240 tggcttcctt cgaagcaagc caggctgcta ctgcggcgat ctcctccggt
tcgccgatat 300 atcccattgg aatcatgctt tctacatcag ctttctgttt
agggtcagcg aatttttcag 360 cattgattgg cgtgttgatc gcacctggcc
caatattatt gacgcgaatg cccttcggcg 420 cgtattccaa cgctaatgtt
tctgtcatca gctttatccc gcctttactt gccgcatagt 480 ggacaaataa
cggccaagga atcacttcgt gcacactgga catgttaatg acatttccct 540
tgatatcgtt ttctacgaaa tatttaatcg cttcacggct tcctaaaaag gcacccgtta
600 agttcgtgcc gatgacttta tcccaatcct tgagcggcat ttcgtgagat
ggcacaggat 660 tttcaagacc ggcattatta atcataatat cgagtgtgcc
gaactcctta attgccgttt 720 gcacgatatt ttttacatct tcctctttcg
tgacatctcc ttggacgaca acagcttcac 780 cgcccgcctt gatgacctct
tcttttacct cgttcggatc ttgtttatta ctataatagt 840 tgataaccac
ttttgcctgc tccttgccga agcgaatggc catcgccttt ccgagccctg 900
aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacatg tatatctcct
960 tgcggccgct cagaactgtg tcgggcgcat caccgcatca atgccgccat
caatgacgat 1020 ctgcgcgcca tgcacatagc ttgcggccgg gctcatcaaa
aaggcgatga ccgacgccat 1080 ctcggacggc tcggcacggc ggcccatggg
aggaacgaac ttggcaatgg attcgccata 1140 gcgcgggtcc tgcaggcccg
cctgcagcaa gggagtctcg gttgcaccgg gggcgatggt 1200 gttcaggcgc
acgccagcct cgccccaggc ggcggcgcgt ttgcgcacag ccaccgtcaa 1260
agcattcttg ctgcccgcat aggccagatt tccgccctgc tctcccgcat gttcgacaat
1320 ggcgcgggcc ttggcttcct cgccggcttc cagtgccagc gccagtgggt
tcttgtcaaa 1380 agccagatgc gcggaagcca cggacgagat gacgacggct
gcgggctgat ggcctttttt 1440 cagcgctggc aaaaaggcat ccatcagctc
ggtcgcgcca aaataattga ccgaaaccac 1500 attgccaagc accttggtct
gcggtcccag gccggcgcac agcaccaggc cgtccatgcc 1560 cttgctgcac
ttcgccagta catcggcaat cgcctgcttt cgaccttcgg ccgtcgagag 1620
atcggcaatc acttccgcat cgcgtatatc gatgcctacg atctggtgac cggccgcctc
1680 caggaccttg cgcgtagcgg caccaatgcc ggtggcgcag ccgcttatca
cgatgatgga 1740 catcatatgt atatctcctt cttatactta actaatatac
taagatgggg aattgttatc 1800 cgctcacaat tcccctatag tgagtcgtat
taatttcgat tatgcggccg tgtacaatac 1860 gattactttc tgttcgactt
aagcattata agcttctagt cgcggtagaa cgaccccatg 1920 tagcggatgt
actcgtcctc ggtgtggttt gccttccagt cgtggacgga gtccttgttg 1980
cgctggccgg cgatgacgga gagctcctta cccagcttct caaaggcctc gtcaacgcac
2040 tcctcggggg tgagggcgat cttcatgacg gcctcgccct gcgggccgcc
ggggaggttg 2100 gacagcaggc tgggggttag ggtggtgccg agggtgatga
cctcgacgtc gacgccggtg 2160 ccctcgcact cgcaggccac ggcctcggtc
atcttgagga tgaaggcctt gcccgcgccg 2220 tactggccgt tccaggggct
ggagctgatg ccggtcatcg acgagacgtt gatcacggcg 2280 ccgcggtcct
gggcggcaaa gatccgcatg tagtggtgga agcacttgag gaaggtcacg 2340
acgttgacgt tgatcatggc ctcgtgcttc tcccaggggg tgtcctggat cttaccgaag
2400 ctgtgcaggc aggccacgta gctcatgaag cccatgtcca ggccctcggt
cgcggcgaag 2460 acggtctcgg cagcgccggg ctggctaaag tcggcgcgca
cgaccttggt ctccacgccg 2520 taggtctcgc ggatctcgcc tgcgagcacg
ttcagcttct cctcgcgacg ggcgaccatg 2580 acgacgttca tgccgccggc
ggcgatcttc tcgcagaacg ccttgccgac gccctcggtc 2640 gcgcccagga
tcaggcccca ctcaccgtac ttctccctca ggttcatatg tatatctcct 2700
tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta
2760 tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
gacgcatcgt 2820 ggccggcatc accggcgcca caggtgcggt tgctggcgcc
tatatcgccg acatcaccga 2880 tggggaagat cgggctcgcc acttcgggct
catgagcgct tgtttcggcg tgggtatggt 2940 ggcaggcccc gtggccgggg
gactgttggg cgccatctcc ttgcatgcac cattccttgc 3000 ggcggcggtg
ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca 3060
taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
3120 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
gtaacgttat 3180 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
ttcccgcgtg gtgaaccagg 3240 ccagccacgt ttctgcgaaa acgcgggaaa
aagtggaagc ggcgatggcg gagctgaatt 3300 acattcccaa ccgcgtggca
caacaactgg cgggcaaaca gtcgttgctg attggcgttg 3360 ccacctccag
tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg 3420
ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
3480 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
attaactatc 3540 cgctggatga ccaggatgcc attgctgtgg aagctgcctg
cactaatgtt ccggcgttat 3600 ttcttgatgt ctctgaccag acacccatca
acagtattat tttctcccat gaagacggta 3660 cgcgactggg cgtggagcat
ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg 3720 gcccattaag
ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc 3780
gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
3840 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
gttgccaacg 3900 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
cgggctgcgc gttggtgcgg 3960 atatctcggt agtgggatac gacgataccg
aagacagctc atgttatatc ccgccgttaa 4020 ccaccatcaa acaggatttt
cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 4080 tctctcaggg
ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 4140
aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
4200 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
cgcaattaat 4260 gtaagttagc tcactcatta ggcaccggga tctcgaccga
tgcccttgag agccttcaac 4320 ccagtcagct ccttccggtg ggcgcggggc
atgactatcg tcgccgcact tatgactgtc 4380 ttctttatca tgcaactcgt
aggacaggtg ccggcagcgc tctgggtcat tttcggcgag 4440 gaccgctttc
gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg 4500
cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
4560 gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct
gtcgttgagg 4620 acccggctag gctggcgggg ttgccttact ggttagcaga
atgaatcacc gatacgcgag 4680 cgaacgtgaa gcgactgctg ctgcaaaacg
tctgcgacct gagcaacaac atgaatggtc 4740 ttcggtttcc gtgtttcgta
aagtctggaa acgcggaagt cagcgccctg caccattatg 4800 ttccggatct
gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta 4860
acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
4920 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
aacccgtatc 4980 gtgagcatcc tctctcgttt catcggtatc attaccccca
tgaacagaaa tcccccttac 5040 acggaggcat cagtgaccaa acaggaaaaa
accgccctta acatggcccg ctttatcaga 5100 agccagacat taacgcttct
ggagaaactc aacgagctgg acgcggatga acaggcagac 5160 atctgtgaat
cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc 5220
ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
5280 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
tggcgggtgt 5340 cggggcgcag ccatgaccca gtcacgtagc gatagcggag
tgtatactgg cttaactatg 5400 cggcatcaga gcagattgta ctgagagtgc
accatatatg cggtgtgaaa taccgcacag 5460 atgcgtaagg agaaaatacc
gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 5520 gcgctcggtc
gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 5580
atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
5640 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
cccctgacga 5700 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
ccgacaggac tataaagata 5760 ccaggcgttt ccccctggaa gctccctcgt
gcgctctcct gttccgaccc tgccgcttac 5820 cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg 5880 taggtatctc
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 5940
cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
6000 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
cgaggtatgt 6060 aggcggtgct acagagttct tgaagtggtg gcctaactac
ggctacacta gaaggacagt 6120 atttggtatc tgcgctctgc tgaagccagt
taccttcgga aaaagagttg gtagctcttg 6180 atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac 6240 gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 6300
gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
6360 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
atgagtaaac 6420 ttggtctgac agttaccaat gcttaatcag tgaggcacct
atctcagcga tctgtctatt 6480 tcgttcatcc atagttgcct gactccccgt
cgtgtagata actacgatac gggagggctt 6540 accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt 6600 atcagcaata
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 6660
cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
6720 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
cgtcgtttgg 6780 tatggcttca ttcagctccg gttcccaacg atcaaggcga
gttacatgat cccccatgtt 6840 gtgcaaaaaa gcggttagct ccttcggtcc
tccgatcgtt gtcagaagta agttggccgc 6900 agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt 6960 aagatgcttt
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7020
gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
7080 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
ggatcttacc 7140 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
aactgatctt cagcatcttt 7200 tactttcacc agcgtttctg ggtgagcaaa
aacaggaagg caaaatgccg caaaaaaggg 7260 aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag 7320 catttatcag
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 7380
acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa
7440 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
accaataggc 7500 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
gagatagggt tgagtgttgt 7560 tccagtttgg aacaagagtc cactattaaa
gaacgtggac tccaacgtca aagggcgaaa 7620 aaccgtctat cagggcgatg
gcccactacg tgaaccatca ccctaatcaa gttttttggg 7680 gtcgaggtgc
cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 7740
acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
7800 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
ccgcgcttaa 7860 tgcgccgcta cagggcgcgt cccattcgcc a 7891 <210>
SEQ ID NO 68 <211> LENGTH: 7404 <212> TYPE: DNA
<213> ORGANISM: artificial <220> FEATURE: <223>
OTHER INFORMATION: pF(G)r7(A) <400> SEQUENCE: 68 atccggatat
agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
gcaagcttct 180 agtcgcggta gaacgacccc atgtagcgga tgtactcgtc
ctcggtgtgg tttgccttcc 240 agtcgtggac ggagtccttg ttgcgctggc
cggcgatgac ggagagctcc ttacccagct 300 tctcaaaggc ctcgtcaacg
cactcctcgg gggtgagggc gatcttcatg acggcctcgc 360 cctgcgggcc
gccggggagg ttggacagca ggctgggggt tagggtggtg ccgagggtga 420
tgacctcgac gtcgacgccg gtgccctcgc actcgcaggc cacggcctcg gtcatcttga
480 ggatgaaggc cttgcccgcg ccgtactggc cgttccaggg gctggagctg
atgccggtca 540 tcgacgagac gttgatcacg gcgccgcggt cctgggcggc
aaagatccgc atgtagtggt 600 ggaagcactt gaggaaggtc acgacgttga
cgttgatcat ggcctcgtgc ttctcccagg 660 gggtgtcctg gatcttaccg
aagctgtgca ggcaggccac gtagctcatg aagcccatgt 720 ccaggccctc
ggtcgcggcg aagacggtct cggcagcgcc gggctggcta aagtcggcgc 780
gcacgacctt ggtctccacg ccgtaggtct cgcggatctc gcctgcgagc acgttcagct
840 tctcctcgcg acgggcgacc atgacgacgt tcatgccgcc ggcggcgatc
ttctcgcaga 900 acgccttgcc gacgccctcg gtcgcgccca ggatcaggcc
ccactcaccg tacttctccc 960 tcaggttcat gtatatctcc tttcacgaat
tctcagccgc cttcttgaac ttggcggcct 1020 cttccgaacc gccggtggca
ttgcccttcg agtaggaatg cgcgccggtg ccggcaagag 1080 cgccgccctg
cacgatgagg tattcgtcgc ggatcggacg gccctcgaag aagcactcca 1140
ggatctcgcg ggtgcccgcc gcataacgcg cctgcgcggt cagcgtggtg ccggagatgt
1200 gcggggtcat gccgttatag ggcatcgtcc gccaggggtg gtccttcggc
gccggctgcg 1260 ggaaccacac gtcgccggca tagccggcca gccggccgga
ttcgagcgca cgtgccacgg 1320 catcgcggtc gcacagcttg ccgcgggcgg
tgttgacgat gtaggcgcca cgcttgaaca 1380 gcttcagcgt ctcgtcattg
atcatgtgct cggtttcggg gtgcagcggg cagttcagcg 1440 tcaccacgtc
gcaaaccgga tacatgtcct cgcgggtcgc gtgccaggtg aggttgagct 1500
ccttctcgac cgattccggc aggcggtgac ggccggtgta gtgcaggtgc acgtcgaacg
1560 gcgccagacg gcgcagcacc gcgagaccga tgcggccggc ggccacggtg
ccgacatgca 1620 tcgcctcgag gtcgtaggcg tgggagacgc agtcggcgat
gttccagccg cccttccgcg 1680 cccattcgtg cgagggcaga tagttgcgca
ccagcgacag gatcatcatc accacatgct 1740 cggcgacgct gatcgagttg
cagtaggtga cttccgccac ggtgacgttg cggtcgatag 1800 ccgactgaag
atcgacgtgg tcggaaccga tgccggcggt gagcgcgagc ttcaggttct 1860
tggccttggc gatgcgctcg ggcgtcagat aggccggcca gaagggctgg gagatgacga
1920 catccgcatc gaccagctcg cgctcgaaca ccgagtcggg gccgtccttg
tcggaggtca 1980 cgaccagggt gtggccgttg gattcgagat attcgcgcag
gccgagctcg ccggagacgg 2040 agccgagcaa ctgcccgggc gtgaagtcga
tggccttcgg cgtcggcaag atctggccgc 2100 ccggatagtg gtcgatcttc
ggaagatcgt cgcgggcata ggtcttcggg tagccgtcga 2160 ccggatcatc
gtaaagaacg cacaggacct ttgccatcat atgtatatct ccttcttaaa 2220
gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
2280 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat
cgtggccggc 2340 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
ccgacatcac cgatggggaa 2400 gatcgggctc gccacttcgg gctcatgagc
gcttgtttcg gcgtgggtat ggtggcaggc 2460 cccgtggccg ggggactgtt
gggcgccatc tccttgcatg caccattcct tgcggcggcg 2520 gtgctcaacg
gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 2580
gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
2640 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
tatacgatgt 2700 cgcagagtat gccggtgtct cttatcagac cgtttcccgc
gtggtgaacc aggccagcca 2760 cgtttctgcg aaaacgcggg aaaaagtgga
agcggcgatg gcggagctga attacattcc 2820 caaccgcgtg gcacaacaac
tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 2880 cagtctggcc
ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 2940
actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
3000 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
atccgctgga 3060 tgaccaggat gccattgctg tggaagctgc ctgcactaat
gttccggcgt tatttcttga 3120 tgtctctgac cagacaccca tcaacagtat
tattttctcc catgaagacg gtacgcgact 3180 gggcgtggag catctggtcg
cattgggtca ccagcaaatc gcgctgttag cgggcccatt 3240 aagttctgtc
tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 3300
aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
3360 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
acgatcagat 3420 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
cgcgttggtg cggatatctc 3480 ggtagtggga tacgacgata ccgaagacag
ctcatgttat atcccgccgt taaccaccat 3540 caaacaggat tttcgcctgc
tggggcaaac cagcgtggac cgcttgctgc aactctctca 3600 gggccaggcg
gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 3660
cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
3720 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
aatgtaagtt 3780 agctcactca ttaggcaccg ggatctcgac cgatgccctt
gagagccttc aacccagtca 3840 gctccttccg gtgggcgcgg ggcatgacta
tcgtcgccgc acttatgact gtcttcttta 3900 tcatgcaact cgtaggacag
gtgccggcag cgctctgggt cattttcggc gaggaccgct 3960 ttcgctggag
cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 4020
tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
4080 tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg
aggacccggc 4140 taggctggcg gggttgcctt actggttagc agaatgaatc
accgatacgc gagcgaacgt 4200 gaagcgactg ctgctgcaaa acgtctgcga
cctgagcaac aacatgaatg gtcttcggtt 4260 tccgtgtttc gtaaagtctg
gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga 4320 tctgcatcgc
aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 4380
gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
4440 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
atcgtgagca 4500 tcctctctcg tttcatcggt atcattaccc ccatgaacag
aaatccccct tacacggagg 4560 catcagtgac caaacaggaa aaaaccgccc
ttaacatggc ccgctttatc agaagccaga 4620 cattaacgct tctggagaaa
ctcaacgagc tggacgcgga tgaacaggca gacatctgtg 4680 aatcgcttca
cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 4740
acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
4800 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
tgtcggggcg 4860 cagccatgac ccagtcacgt agcgatagcg gagtgtatac
tggcttaact atgcggcatc 4920 agagcagatt gtactgagag tgcaccatat
atgcggtgtg aaataccgca cagatgcgta 4980 aggagaaaat accgcatcag
gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 5040 gtcgttcggc
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 5100
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
5160 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
cgagcatcac 5220 aaaaatcgac gctcaagtca gaggtggcga aacccgacag
gactataaag ataccaggcg 5280 tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac 5340 ctgtccgcct ttctcccttc
gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 5400 ctcagttcgg
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5460
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
5520 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
tgtaggcggt 5580 gctacagagt tcttgaagtg gtggcctaac tacggctaca
ctagaaggac agtatttggt 5640 atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc 5700 aaacaaacca ccgctggtag
cggtggtttt tttgtttgca agcagcagat tacgcgcaga 5760 aaaaaaggat
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5820
gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5880 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
aacttggtct 5940 gacagttacc aatgcttaat cagtgaggca cctatctcag
cgatctgtct atttcgttca 6000 tccatagttg cctgactccc cgtcgtgtag
ataactacga tacgggaggg cttaccatct 6060 ggccccagtg ctgcaatgat
accgcgagac ccacgctcac cggctccaga tttatcagca 6120 ataaaccagc
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6180
atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
6240 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
tggtatggct 6300 tcattcagct ccggttccca acgatcaagg cgagttacat
gatcccccat gttgtgcaaa 6360 aaagcggtta gctccttcgg tcctccgatc
gttgtcagaa gtaagttggc cgcagtgtta 6420 tcactcatgg ttatggcagc
actgcataat tctcttactg tcatgccatc cgtaagatgc 6480 ttttctgtga
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6540
agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
6600 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
accgctgttg 6660 agatccagtt cgatgtaacc cactcgtgca cccaactgat
cttcagcatc ttttactttc 6720 accagcgttt ctgggtgagc aaaaacagga
aggcaaaatg ccgcaaaaaa gggaataagg 6780 gcgacacgga aatgttgaat
actcatactc ttcctttttc aatattattg aagcatttat 6840 cagggttatt
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6900
ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg
6960 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
ggccgaaatc 7020
ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
7080 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
aaaaaccgtc 7140 tatcagggcg atggcccact acgtgaacca tcaccctaat
caagtttttt ggggtcgagg 7200 tgccgtaaag cactaaatcg gaaccctaaa
gggagccccc gatttagagc ttgacgggga 7260 aagccggcga acgtggcgag
aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 7320 ctggcaagtg
tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 7380
ctacagggcg cgtcccattc gcca 7404 <210> SEQ ID NO 69
<211> LENGTH: 7386 <212> TYPE: DNA <213>
ORGANISM: artificial <220> FEATURE: <223> OTHER
INFORMATION: pFr3 <400> SEQUENCE: 69 atccggatat agttcctcct
ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60 ggggttatgc
tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120
tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc
180 agaactgtgt cgggcgcatc accgcatcaa tgccgccatc aatgacgatc
tgcgcgccat 240 gcacatagct tgcggccggg ctcatcaaaa aggcgatgac
cgacgccatc tcggacggct 300 cggcacggcg gcccatggga ggaacgaact
tggcaatgga ttcgccatag cgcgggtcct 360 gcaggcccgc ctgcagcaag
ggagtctcgg ttgcaccggg ggcgatggtg ttcaggcgca 420 cgccagcctc
gccccaggcg gcggcgcgtt tgcgcacagc caccgtcaaa gcattcttgc 480
tgcccgcata ggccagattt ccgccctgct ctcccgcatg ttcgacaatg gcgcgggcct
540 tggcttcctc gccggcttcc agtgccagcg ccagtgggtt cttgtcaaaa
gccagatgcg 600 cggaagccac ggacgagatg acgacggctg cgggctgatg
gccttttttc agcgctggca 660 aaaaggcatc catcagctcg gtcgcgccaa
aataattgac cgaaaccaca ttgccaagca 720 ccttggtctg cggtcccagg
ccggcgcaca gcaccaggcc gtccatgccc ttgctgcact 780 tcgccagtac
atcggcaatc gcctgctttc gaccttcggc cgtcgagaga tcggcaatca 840
cttccgcatc gcgtatatcg atgcctacga tctggtgacc ggccgcctcc aggaccttgc
900 gcgtagccgc accaatgccg gtggcgcagc cgcttatcac gatgatggac
atgtatatct 960 cctttcacga attctcagcc gccttcttga acttggcggc
ctcttccgaa ccgccggtgg 1020 cattgccctt cgagtaggaa tgcgcgccgg
tgccggcaag agcgccgccc tgcacgatga 1080 ggtattcgtc gcggatcgga
cggccctcga agaagcactc caggatctcg cgggtgcccg 1140 ccgcataacg
cgcctgcgcg gtcagcgtgg tgccggagat gtgcggggtc atgccgttat 1200
agggcatcgt ccgccagggg tggtccttcg gcgccggctg cgggaaccac acgtcgccgg
1260 catagccggc cagccggccg gattcgagcg cacgtgccac ggcatcgcgg
tcgcacagct 1320 tgccgcgggc ggtgttgacg atgtaggcgc cacgcttgaa
cagcttcagc gtctcgtcat 1380 tgatcatgtg ctcggtttcg gggtgcagcg
ggcagttcag cgtcaccacg tcgcaaaccg 1440 gatacatgtc ctcgcgggtc
gcgtgccagg tgaggttgag ctccttctcg accgattccg 1500 gcaggcggtg
acggtcggtg tagtgcaggt gcacgtcgaa cggcgccaga cggcgcagca 1560
ccgcgagacc gatgcggccg gcggccacgg tgccgacatg catcgcctcg aggtcgtagg
1620 cgtgggagac gcagtcggcg atgttccagc cgcccttccg cgcccattcg
tgcgagggca 1680 gatagttgcg caccagcgac aggatcatca tcaccacatg
ctcggcgacg ctgatcgagt 1740 tgcagtaggt gacttccgcc acggtgacgt
tgcggtcgat agccgactga agatcgacgt 1800 ggtcggaacc gatgccggcg
gtgagcgcga gcttcaggtt cttggccttg gcgatgcgct 1860 cgggcgtcag
ataggccggc cagaagggct gggagatgac gacatccgca tcgaccagct 1920
cgcgctcgaa caccgagtcg gggccgtcct tgtcggaggt cacgaccagg gtgtggccgt
1980 tggattcgag atattcgcgc aggccgagct cgccggagac ggagccgagc
aactgcccgg 2040 gcgtgaagtc gatggccttc ggcgtcggca agatctggcc
gcccggatag tggtcgatct 2100 tcggaagatc gtcgcgggca taggtcttcg
ggtagccgtc gaccggatca tcgtaaagaa 2160 cgcacaggac ctttgccatc
atatgtatat ctccttctta aagttaaaca aaattatttc 2220 tagaggggaa
ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg 2280
atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
2340 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
tcgccacttc 2400 gggctcatga gcgcttgttt cggcgtgggt atggtggcag
gccccgtggc cgggggactg 2460 ttgggcgcca tctccttgca tgcaccattc
cttgcggcgg cggtgctcaa cggcctcaac 2520 ctactactgg gctgcttcct
aatgcaggag tcgcataagg gagagcgtcg agatcccgga 2580 caccatcgaa
tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca 2640
attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
2700 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
cgaaaacgcg 2760 ggaaaaagtg gaagcggcga tggcggagct gaattacatt
cccaaccgcg tggcacaaca 2820 actggcgggc aaacagtcgt tgctgattgg
cgttgccacc tccagtctgg ccctgcacgc 2880 gccgtcgcaa attgtcgcgg
cgattaaatc tcgcgccgat caactgggtg ccagcgtggt 2940 ggtgtcgatg
gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct 3000
cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
3060 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
accagacacc 3120 catcaacagt attattttct cccatgaaga cggtacgcga
ctgggcgtgg agcatctggt 3180 cgcattgggt caccagcaaa tcgcgctgtt
agcgggccca ttaagttctg tctcggcgcg 3240 tctgcgtctg gctggctggc
ataaatatct cactcgcaat caaattcagc cgatagcgga 3300 acgggaaggc
gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga 3360
gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
3420 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
gatacgacga 3480 taccgaagac agctcatgtt atatcccgcc gttaaccacc
atcaaacagg attttcgcct 3540 gctggggcaa accagcgtgg accgcttgct
gcaactctct cagggccagg cggtgaaggg 3600 caatcagctg ttgcccgtct
cactggtgaa aagaaaaacc accctggcgc ccaatacgca 3660 aaccgcctct
ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 3720
actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac
3780 cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc
cggtgggcgc 3840 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
tatcatgcaa ctcgtaggac 3900 aggtgccggc agcgctctgg gtcattttcg
gcgaggaccg ctttcgctgg agcgcgacga 3960 tgatcggcct gtcgcttgcg
gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca 4020 ctggtcccgc
caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc 4080
cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
4140 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
tgctgctgca 4200 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
tttccgtgtt tcgtaaagtc 4260 tggaaacgcg gaagtcagcg ccctgcacca
ttatgttccg gatctgcatc gcaggatgct 4320 gctggctacc ctgtggaaca
cctacatctg tattaacgaa gcgctggcat tgaccctgag 4380 tgatttttct
ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 4440
gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
4500 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
accaaacagg 4560 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
gacattaacg cttctggaga 4620 aactcaacga gctggacgcg gatgaacagg
cagacatctg tgaatcgctt cacgaccacg 4680 ctgatgagct ttaccgcagc
tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 4740 acatgcagct
cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 4800
cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
4860 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
ttgtactgag 4920 agtgcaccat atatgcggtg tgaaataccg cacagatgcg
taaggagaaa ataccgcatc 4980 aggcgctctt ccgcttcctc gctcactgac
tcgctgcgct cggtcgttcg gctgcggcga 5040 gcggtatcag ctcactcaaa
ggcggtaata cggttatcca cagaatcagg ggataacgca 5100 ggaaagaaca
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 5160
ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5220 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
tggaagctcc 5280 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
acctgtccgc ctttctccct 5340 tcgggaagcg tggcgctttc tcatagctca
cgctgtaggt atctcagttc ggtgtaggtc 5400 gttcgctcca agctgggctg
tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 5460 tccggtaact
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 5520
gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5580 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
tctgctgaag 5640 ccagttacct tcggaaaaag agttggtagc tcttgatccg
gcaaacaaac caccgctggt 5700 agcggtggtt tttttgtttg caagcagcag
attacgcgca gaaaaaaagg atctcaagaa 5760 gatcctttga tcttttctac
ggggtctgac gctcagtgga acgaaaactc acgttaaggg 5820 attttggtca
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 5880
agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5940 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
tgcctgactc 6000 cccgtcgtgt agataactac gatacgggag ggcttaccat
ctggccccag tgctgcaatg 6060 ataccgcgag acccacgctc accggctcca
gatttatcag caataaacca gccagccgga 6120 agggccgagc gcagaagtgg
tcctgcaact ttatccgcct ccatccagtc tattaattgt 6180 tgccgggaag
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 6240
gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6300 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
tagctccttc 6360 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
tatcactcat ggttatggca 6420 gcactgcata attctcttac tgtcatgcca
tccgtaagat gcttttctgt gactggtgag 6480 tactcaacca agtcattctg
agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 6540 tcaatacggg
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 6600
cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6660 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
ttctgggtga 6720 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
gggcgacacg gaaatgttga 6780 atactcatac tcttcctttt tcaatattat
tgaagcattt atcagggtta ttgtctcatg 6840
agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6900 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt
cgcgttaaat 6960 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
tcggcaaaat cccttataaa 7020 tcaaaagaat agaccgagat agggttgagt
gttgttccag tttggaacaa gagtccacta 7080 ttaaagaacg tggactccaa
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 7140 ctacgtgaac
catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 7200
cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
7260 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
tgtagcggtc 7320 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
cgctacaggg cgcgtcccat 7380 tcgcca 7386
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