U.S. patent application number 13/821001 was filed with the patent office on 2014-02-27 for use of biological or chemical control agents for controlling insects and nematodes in resistant crops. This patent application is currently assigned to BAYER INTELLECTUAL PROPERTY GMBH. The applicant listed for this patent is Wolfram Andersch, Kevin Bugg, Nalini Desai, Candace Poutre, Jennifer Riggs, Steven Riniker, Carrie Russell, Bill Striegel, Julia Thissen Daum. Invention is credited to Wolfram Andersch, Kevin Bugg, Nalini Desai, Candace Poutre, Jennifer Riggs, Steven Riniker, Carrie Russell, Bill Striegel, Julia Thissen Daum.
Application Number | 20140056866 13/821001 |
Document ID | / |
Family ID | 45873472 |
Filed Date | 2014-02-27 |
United States Patent Application | 20140056866 |
Kind Code | A1 |
Andersch; Wolfram ; et al. | February 27, 2014 |
The present invention relates generally to the use of biological or chemical control agents with for controlling insects and nematodes and to methods particularly useful for combating insects or nematodes and/or increasing crop yield in plants that are at least partially resistant to one or more parasitic nematodes or insects.
Inventors: | Andersch; Wolfram; (Bergisch Gladbach, DE) ; Riggs; Jennifer; (Raleigh, NC) ; Poutre; Candace; (Moncure, NC) ; Desai; Nalini; (Chapel Hill, NC) ; Striegel; Bill; (Cary, NC) ; Bugg; Kevin; (Raliegh, NC) ; Riniker; Steven; (Seffner, FL) ; Russell; Carrie; (Latham, NY) ; Thissen Daum; Julia; (Apex, NC) | ||||||||||
Applicant: |
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Assignee: | BAYER INTELLECTUAL PROPERTY
GMBH Monheim DE |
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Family ID: | 45873472 | ||||||||||
Appl. No.: | 13/821001 | ||||||||||
Filed: | September 21, 2011 | ||||||||||
PCT Filed: | September 21, 2011 | ||||||||||
PCT NO: | PCT/EP2011/066453 | ||||||||||
371 Date: | May 24, 2013 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
61385247 | Sep 22, 2010 | |||
61418520 | Dec 1, 2010 | |||
61419433 | Dec 3, 2010 | |||
Current U.S. Class: | 424/93.461 |
Current CPC Class: | A01N 43/40 20130101; A01N 63/00 20130101; A01N 43/40 20130101; A01N 63/00 20130101; A01N 63/30 20200101; A01N 2300/00 20130101; A01N 43/40 20130101; A01N 2300/00 20130101; A01N 63/30 20200101; A01N 63/30 20200101; A01N 63/00 20130101 |
Class at Publication: | 424/93.461 |
International Class: | A01N 63/04 20060101 A01N063/04; A01N 63/00 20060101 A01N063/00 |
Date | Code | Application Number |
---|---|---|
Dec 1, 2010 | EP | 10193328.1 |
Dec 1, 2010 | EP | 10193341.4 |
Sequence CWU 1
1
7511608DNAChromobacterium sp. 1atggcatccg cagcaaatgc aggtcagctt
ggcaacctcc ccggcgttac ttccatgggc 60atgggctatg acgtgaatgg tttgtacgcc
agcccggaaa gcctgcttgg ccaacccttg 120ttcgatttcg gcggcgagct
ggacagcatc gaaatcgagg gccgcagcta cacctttccc 180cgcagcatgc
atgtacacac ctatttccat tccgacttca aacaggatgt cagcaaggaa
240atcgaagagt atcgggagaa aatgagccag cacgtgggcg tgtccggccg
ctacaagttg 300ttcagcgctt cgctgagcgt ggatttcacc accacggacc
agcaactgac cgagattacc 360tacagctcca cccgcgaagc ccatgtgctg
tggtacatca gcctgcctgg cgcggccacg 420ctgcgttcga tgctgcgccg
cgatttccgc gacgacctga acaaccccaa tatgccggcc 480atggagctgt
tcaagcgcta tggtccctac tacatatcgg aagcggcggt gggcggccgg
540ctggactaca gcgcggccag caagaccttg aagatggaca gcagccagtc
gctgtccacc 600accgccgaaa tgtcctacaa ggcgctggtg ggcgagatca
agatcgagca tggctcggag 660atggaaaagc aggtcaacag cttccgcagc
aactccacca tccgtctcac cgccaccggc 720ggcaagccgg gcatgaccga
tcgcatactg cacggtccgg attcgcagca ggcgttctcg 780caatgggcgg
aatcgctgct cgactatgcg acgctgatgg acttttccac cgaaagcctg
840caaccgatct gggcgctggc cgacaagccc gagcgccgcg tcgagcttga
ggacgccttc 900cccgaattca tgaagcagtc gcagcagtcc atccccaagg
tggacaaggt gctgctgatg 960gacgcgcggc cgcctatggt gaaggctggg
gaggatagcg gctccggcgc gtcggaggat 1020ctggctgtgt tcaatcccag
cacctccaat ggctacaaga tggttggcca gttcggtcag 1080cgcaaccatg
ccagcgtggc ggatggccat gcgccgattt tcaaggatct gttcgatctg
1140ggcgtgctga aggcgccggt gggttggcag cgggtgtggg acgacgccgg
ctccggcaag 1200tccaaggact acgcgtgctg gcgcgcgatt ccgccgcagg
gctaccgcgc gctgggcgat 1260gtgatgatgc tggccaccag cggctataac
ccgccgaatc tgccggacta tgtttgcgtg 1320catcaaagcc tgtgcgcgga
tgtgcagacg ctgcaaaacc gggtgtggtg ggacaagggc 1380accggcgcgc
gcaaggatgt cagcctgtgg caaccgggcg cggccggcgc ggtggcgtcc
1440tcttgcttcg ccggcgtgcc taattacaac aacccgccca attccggcga
catcgagcgc 1500ttgcgcggca gcatcgcatg cgtgaagacc agcgcgatcg
cgtccatgca ggaaatgaag 1560tccatgctca gccagcacca aggcatggaa
gcgatgatgt ccaagctg 16082536PRTChromobacterium sp. 2Met Ala Ser Ala
Ala Asn Ala Gly Gln Leu Gly Asn Leu Pro Gly Val 1 5 10 15 Thr Ser
Met Gly Met Gly Tyr Asp Val Asn Gly Leu Tyr Ala Ser Pro 20 25 30
Glu Ser Leu Leu Gly Gln Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp 35
40 45 Ser Ile Glu Ile Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser Met
His 50 55 60 Val His Thr Tyr Phe His Ser Asp Phe Lys Gln Asp Val
Ser Lys Glu 65 70 75 80 Ile Glu Glu Tyr Arg Glu Lys Met Ser Gln His
Val Gly Val Ser Gly 85 90 95 Arg Tyr Lys Leu Phe Ser Ala Ser Leu
Ser Val Asp Phe Thr Thr Thr 100 105 110 Asp Gln Gln Leu Thr Glu Ile
Thr Tyr Ser Ser Thr Arg Glu Ala His 115 120 125 Val Leu Trp Tyr Ile
Ser Leu Pro Gly Ala Ala Thr Leu Arg Ser Met 130 135 140 Leu Arg Arg
Asp Phe Arg Asp Asp Leu Asn Asn Pro Asn Met Pro Ala 145 150 155 160
Met Glu Leu Phe Lys Arg Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala 165
170 175 Val Gly Gly Arg Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu Lys
Met 180 185 190 Asp Ser Ser Gln Ser Leu Ser Thr Thr Ala Glu Met Ser
Tyr Lys Ala 195 200 205 Leu Val Gly Glu Ile Lys Ile Glu His Gly Ser
Glu Met Glu Lys Gln 210 215 220 Val Asn Ser Phe Arg Ser Asn Ser Thr
Ile Arg Leu Thr Ala Thr Gly 225 230 235 240 Gly Lys Pro Gly Met Thr
Asp Arg Ile Leu His Gly Pro Asp Ser Gln 245 250 255 Gln Ala Phe Ser
Gln Trp Ala Glu Ser Leu Leu Asp Tyr Ala Thr Leu 260 265 270 Met Asp
Phe Ser Thr Glu Ser Leu Gln Pro Ile Trp Ala Leu Ala Asp 275 280 285
Lys Pro Glu Arg Arg Val Glu Leu Glu Asp Ala Phe Pro Glu Phe Met 290
295 300 Lys Gln Ser Gln Gln Ser Ile Pro Lys Val Asp Lys Val Leu Leu
Met 305 310 315 320 Asp Ala Arg Pro Pro Met Val Lys Ala Gly Glu Asp
Ser Gly Ser Gly 325 330 335 Ala Ser Glu Asp Leu Ala Val Phe Asn Pro
Ser Thr Ser Asn Gly Tyr 340 345 350 Lys Met Val Gly Gln Phe Gly Gln
Arg Asn His Ala Ser Val Ala Asp 355 360 365 Gly His Ala Pro Ile Phe
Lys Asp Leu Phe Asp Leu Gly Val Leu Lys 370 375 380 Ala Pro Val Gly
Trp Gln Arg Val Trp Asp Asp Ala Gly Ser Gly Lys 385 390 395 400 Ser
Lys Asp Tyr Ala Cys Trp Arg Ala Ile Pro Pro Gln Gly Tyr Arg 405 410
415 Ala Leu Gly Asp Val Met Met Leu Ala Thr Ser Gly Tyr Asn Pro Pro
420 425 430 Asn Leu Pro Asp Tyr Val Cys Val His Gln Ser Leu Cys Ala
Asp Val 435 440 445 Gln Thr Leu Gln Asn Arg Val Trp Trp Asp Lys Gly
Thr Gly Ala Arg 450 455 460 Lys Asp Val Ser Leu Trp Gln Pro Gly Ala
Ala Gly Ala Val Ala Ser 465 470 475 480 Ser Cys Phe Ala Gly Val Pro
Asn Tyr Asn Asn Pro Pro Asn Ser Gly 485 490 495 Asp Ile Glu Arg Leu
Arg Gly Ser Ile Ala Cys Val Lys Thr Ser Ala 500 505 510 Ile Ala Ser
Met Gln Glu Met Lys Ser Met Leu Ser Gln His Gln Gly 515 520 525 Met
Glu Ala Met Met Ser Lys Leu 530 535 3518PRTChromobacterium sp. 3Met
Gly Met Gly Tyr Asp Val Asn Gly Leu Tyr Ala Ser Pro Glu Ser 1 5 10
15 Leu Leu Gly Gln Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp Ser Ile
20 25 30 Glu Ile Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser Met His
Val His 35 40 45 Thr Tyr Phe His Ser Asp Phe Lys Gln Asp Val Ser
Lys Glu Ile Glu 50 55 60 Glu Tyr Arg Glu Lys Met Ser Gln His Val
Gly Val Ser Gly Arg Tyr 65 70 75 80 Lys Leu Phe Ser Ala Ser Leu Ser
Val Asp Phe Thr Thr Thr Asp Gln 85 90 95 Gln Leu Thr Glu Ile Thr
Tyr Ser Ser Thr Arg Glu Ala His Val Leu 100 105 110 Trp Tyr Ile Ser
Leu Pro Gly Ala Ala Thr Leu Arg Ser Met Leu Arg 115 120 125 Arg Asp
Phe Arg Asp Asp Leu Asn Asn Pro Asn Met Pro Ala Met Glu 130 135 140
Leu Phe Lys Arg Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala Val Gly 145
150 155 160 Gly Arg Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu Lys Met
Asp Ser 165 170 175 Ser Gln Ser Leu Ser Thr Thr Ala Glu Met Ser Tyr
Lys Ala Leu Val 180 185 190 Gly Glu Ile Lys Ile Glu His Gly Ser Glu
Met Glu Lys Gln Val Asn 195 200 205 Ser Phe Arg Ser Asn Ser Thr Ile
Arg Leu Thr Ala Thr Gly Gly Lys 210 215 220 Pro Gly Met Thr Asp Arg
Ile Leu His Gly Pro Asp Ser Gln Gln Ala 225 230 235 240 Phe Ser Gln
Trp Ala Glu Ser Leu Leu Asp Tyr Ala Thr Leu Met Asp 245 250 255 Phe
Ser Thr Glu Ser Leu Gln Pro Ile Trp Ala Leu Ala Asp Lys Pro 260 265
270 Glu Arg Arg Val Glu Leu Glu Asp Ala Phe Pro Glu Phe Met Lys Gln
275 280 285 Ser Gln Gln Ser Ile Pro Lys Val Asp Lys Val Leu Leu Met
Asp Ala 290 295 300 Arg Pro Pro Met Val Lys Ala Gly Glu Asp Ser Gly
Ser Gly Ala Ser 305 310 315 320 Glu Asp Leu Ala Val Phe Asn Pro Ser
Thr Ser Asn Gly Tyr Lys Met 325 330 335 Val Gly Gln Phe Gly Gln Arg
Asn His Ala Ser Val Ala Asp Gly His 340 345 350 Ala Pro Ile Phe Lys
Asp Leu Phe Asp Leu Gly Val Leu Lys Ala Pro 355 360 365 Val Gly Trp
Gln Arg Val Trp Asp Asp Ala Gly Ser Gly Lys Ser Lys 370 375 380 Asp
Tyr Ala Cys Trp Arg Ala Ile Pro Pro Gln Gly Tyr Arg Ala Leu 385 390
395 400 Gly Asp Val Met Met Leu Ala Thr Ser Gly Tyr Asn Pro Pro Asn
Leu 405 410 415 Pro Asp Tyr Val Cys Val His Gln Ser Leu Cys Ala Asp
Val Gln Thr 420 425 430 Leu Gln Asn Arg Val Trp Trp Asp Lys Gly Thr
Gly Ala Arg Lys Asp 435 440 445 Val Ser Leu Trp Gln Pro Gly Ala Ala
Gly Ala Val Ala Ser Ser Cys 450 455 460 Phe Ala Gly Val Pro Asn Tyr
Asn Asn Pro Pro Asn Ser Gly Asp Ile 465 470 475 480 Glu Arg Leu Arg
Gly Ser Ile Ala Cys Val Lys Thr Ser Ala Ile Ala 485 490 495 Ser Met
Gln Glu Met Lys Ser Met Leu Ser Gln His Gln Gly Met Glu 500 505 510
Ala Met Met Ser Lys Leu 515 4516PRTChromobacterium sp. 4Met Gly Tyr
Asp Val Asn Gly Leu Tyr Ala Ser Pro Glu Ser Leu Leu 1 5 10 15 Gly
Gln Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp Ser Ile Glu Ile 20 25
30 Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser Met His Val His Thr Tyr
35 40 45 Phe His Ser Asp Phe Lys Gln Asp Val Ser Lys Glu Ile Glu
Glu Tyr 50 55 60 Arg Glu Lys Met Ser Gln His Val Gly Val Ser Gly
Arg Tyr Lys Leu 65 70 75 80 Phe Ser Ala Ser Leu Ser Val Asp Phe Thr
Thr Thr Asp Gln Gln Leu 85 90 95 Thr Glu Ile Thr Tyr Ser Ser Thr
Arg Glu Ala His Val Leu Trp Tyr 100 105 110 Ile Ser Leu Pro Gly Ala
Ala Thr Leu Arg Ser Met Leu Arg Arg Asp 115 120 125 Phe Arg Asp Asp
Leu Asn Asn Pro Asn Met Pro Ala Met Glu Leu Phe 130 135 140 Lys Arg
Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala Val Gly Gly Arg 145 150 155
160 Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu Lys Met Asp Ser Ser Gln
165 170 175 Ser Leu Ser Thr Thr Ala Glu Met Ser Tyr Lys Ala Leu Val
Gly Glu 180 185 190 Ile Lys Ile Glu His Gly Ser Glu Met Glu Lys Gln
Val Asn Ser Phe 195 200 205 Arg Ser Asn Ser Thr Ile Arg Leu Thr Ala
Thr Gly Gly Lys Pro Gly 210 215 220 Met Thr Asp Arg Ile Leu His Gly
Pro Asp Ser Gln Gln Ala Phe Ser 225 230 235 240 Gln Trp Ala Glu Ser
Leu Leu Asp Tyr Ala Thr Leu Met Asp Phe Ser 245 250 255 Thr Glu Ser
Leu Gln Pro Ile Trp Ala Leu Ala Asp Lys Pro Glu Arg 260 265 270 Arg
Val Glu Leu Glu Asp Ala Phe Pro Glu Phe Met Lys Gln Ser Gln 275 280
285 Gln Ser Ile Pro Lys Val Asp Lys Val Leu Leu Met Asp Ala Arg Pro
290 295 300 Pro Met Val Lys Ala Gly Glu Asp Ser Gly Ser Gly Ala Ser
Glu Asp 305 310 315 320 Leu Ala Val Phe Asn Pro Ser Thr Ser Asn Gly
Tyr Lys Met Val Gly 325 330 335 Gln Phe Gly Gln Arg Asn His Ala Ser
Val Ala Asp Gly His Ala Pro 340 345 350 Ile Phe Lys Asp Leu Phe Asp
Leu Gly Val Leu Lys Ala Pro Val Gly 355 360 365 Trp Gln Arg Val Trp
Asp Asp Ala Gly Ser Gly Lys Ser Lys Asp Tyr 370 375 380 Ala Cys Trp
Arg Ala Ile Pro Pro Gln Gly Tyr Arg Ala Leu Gly Asp 385 390 395 400
Val Met Met Leu Ala Thr Ser Gly Tyr Asn Pro Pro Asn Leu Pro Asp 405
410 415 Tyr Val Cys Val His Gln Ser Leu Cys Ala Asp Val Gln Thr Leu
Gln 420 425 430 Asn Arg Val Trp Trp Asp Lys Gly Thr Gly Ala Arg Lys
Asp Val Ser 435 440 445 Leu Trp Gln Pro Gly Ala Ala Gly Ala Val Ala
Ser Ser Cys Phe Ala 450 455 460 Gly Val Pro Asn Tyr Asn Asn Pro Pro
Asn Ser Gly Asp Ile Glu Arg 465 470 475 480 Leu Arg Gly Ser Ile Ala
Cys Val Lys Thr Ser Ala Ile Ala Ser Met 485 490 495 Gln Glu Met Lys
Ser Met Leu Ser Gln His Gln Gly Met Glu Ala Met 500 505 510 Met Ser
Lys Leu 515 5536PRTArtificial sequenceAXMI-205(evo24) 5Met Ala Ser
Ala Ala Asn Ala Gly Gln Leu Gly Asn Leu Pro Gly Val 1 5 10 15 Thr
Ser Met Gly Met Gly Tyr Asp Val Asn Gly Leu Tyr Ala Ser Pro 20 25
30 Glu Ser Leu Leu Gly Gln Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp
35 40 45 Ser Ile Glu Ile Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser
Met His 50 55 60 Val His Thr Tyr Phe His Ser Asp Phe Lys Gln Asp
Val Ser Lys Glu 65 70 75 80 Ile Glu Glu Tyr Arg Glu Lys Met Ser Gln
His Val Gly Val Ser Gly 85 90 95 Arg Tyr Lys Leu Phe Ser Ala Ser
Leu Ser Val Asp Phe Thr Thr Thr 100 105 110 Asp Gln Gln Leu Thr Glu
Ile Thr Tyr Ser Ser Thr Arg Glu Ala His 115 120 125 Val Leu Trp Tyr
Ile Ser Leu Pro Gly Ala Ala Thr Leu Arg Ser Met 130 135 140 Leu Arg
Arg Asp Phe Arg Asp Asp Leu Asn Asn Pro Asn Met Pro Ala 145 150 155
160 Met Glu Leu Phe Lys Arg Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala
165 170 175 Val Gly Gly Arg Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu
Lys Met 180 185 190 Asp Ser Ser Gln Ser Leu Ser Thr Thr Ala Glu Met
Ser Tyr Lys Ala 195 200 205 Leu Val Gly Glu Ile Lys Ile Glu His Gly
Ser Glu Met Glu Lys Gln 210 215 220 Val Asn Ser Phe Arg Ser Asn Ser
Thr Ile Arg Leu Thr Ala Thr Gly 225 230 235 240 Gly Lys Pro Gly Met
Thr Asp Arg Ile Leu His Gly Pro Asp Ser Gln 245 250 255 Gln Ala Phe
Ser Gln Trp Ala Glu Ser Leu Leu Asp Tyr Ala Thr Leu 260 265 270 Met
Asp Phe Ser Thr Glu Ser Leu Gln Pro Ile Trp Ala Leu Ala Asp 275 280
285 Lys Pro Glu Arg Arg Val Glu Leu Glu Asp Ala Phe Pro Glu Phe Met
290 295 300 Lys Gln Ser Gln Gln Ser Ile Pro Lys Val Asp Lys Val Leu
Leu Met 305 310 315 320 Asp Ala Arg Pro Pro Met Val Lys Ala Gly Glu
Asp Ser Gly Ser Gly 325 330 335 Ala Ser Glu Asp Leu Ala Val Phe Asn
Pro Ser Thr Ser Asn Gly Tyr 340 345 350 Lys Met Val Gly Gln Phe Gly
Gln Arg Asn His Ala Ser Val Ala Asp 355 360 365 Gly His Ala Pro Ile
Phe Lys Asp Leu Phe Asp Leu Gly Val Leu Lys 370 375 380 Ala Pro Val
Gly Trp Gln Arg Val Trp Asp Asp Ala Gly Ser Gly Lys 385 390 395 400
Ser Lys Asp Tyr Ala Cys Trp Arg Ala Ile Pro Pro Gln Gly Tyr Arg 405
410 415 Ala Leu Gly Asp Val Met Met Leu Ala Thr Ser Gly Tyr Asn Pro
Pro 420 425 430 Asn Leu Pro Asp Tyr Val Cys Val His Gln Ser Leu Cys
Ala Asp Val 435 440 445 Gln Thr Leu Gln Asn Arg Val Trp Trp Asp Lys
Gly Thr Gly Ala Arg 450 455 460 Lys Asp Val Ser Leu Trp Gln Pro Gly
Ala Ala
Gly Ala Val Ala Ser 465 470 475 480 Ser Cys Phe Ala Gly Val Pro Asn
Tyr Asn Asn Pro Pro Asn Ser Gly 485 490 495 Asp Ile Ala Arg Leu Arg
Gly Ser Ile Ala Cys Val Lys Thr Ser Ala 500 505 510 Ile Ala Ser Met
Gln Glu Met Lys Ser Met Leu Ser Gln His Gln Gly 515 520 525 Met Glu
Ala Met Met Ser Lys Leu 530 535 6536PRTArtificial
sequenceAXMI-205(evo25) 6Met Ala Ser Ala Ala Asn Ala Gly Gln Leu
Gly Asn Leu Pro Gly Val 1 5 10 15 Thr Ser Met Gly Met Gly Tyr Asp
Val Asn Gly Leu Tyr Ala Ser Pro 20 25 30 Glu Ser Leu Leu Gly Gln
Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp 35 40 45 Ser Ile Glu Ile
Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser Met His 50 55 60 Val His
Thr Tyr Phe His Ser Asp Phe Lys Gln Asp Val Ser Lys Glu 65 70 75 80
Ile Glu Glu Tyr Arg Glu Lys Met Ser Gln His Val Gly Val Ser Gly 85
90 95 Arg Tyr Lys Leu Phe Ser Ala Ser Leu Ser Val Asp Phe Thr Thr
Thr 100 105 110 Asp Gln Gln Leu Thr Glu Ile Thr Tyr Ser Ser Thr Arg
Glu Ala His 115 120 125 Val Leu Trp Tyr Ile Ser Leu Pro Gly Ala Ala
Thr Leu Arg Ser Met 130 135 140 Leu Arg Arg Asp Phe Arg Asp Asp Leu
Asn Asn Pro Asn Met Pro Ala 145 150 155 160 Met Glu Leu Phe Lys Arg
Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala 165 170 175 Val Gly Gly Arg
Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu Lys Met 180 185 190 Asp Ser
Ser Gln Ser Leu Ser Thr Thr Ala Glu Met Ser Tyr Lys Ala 195 200 205
Leu Val Gly Glu Ile Lys Ile Glu His Gly Ser Glu Met Glu Lys Gln 210
215 220 Val Asn Ser Phe Arg Ser Asn Ser Thr Ile Arg Leu Thr Ala Thr
Gly 225 230 235 240 Gly Lys Pro Gly Met Thr Asp Arg Ile Leu His Gly
Pro Asp Ser Gln 245 250 255 Gln Ala Phe Ser Gln Trp Ala Glu Ser Leu
Leu Asp Tyr Ala Thr Leu 260 265 270 Met Asp Phe Ser Thr Glu Ser Leu
Gln Pro Ile Trp Ala Leu Ala Asp 275 280 285 Lys Pro Glu Arg Arg Val
Glu Leu Glu Asp Ala Phe Pro Glu Phe Met 290 295 300 Lys Gln Ser Gln
Gln Ser Ile Pro Lys Val Asp Lys Val Leu Leu Met 305 310 315 320 Asp
Ala Arg Pro Pro Met Val Lys Ala Gly Glu Asp Ser Gly Ser Gly 325 330
335 Ala Ser Glu Asp Leu Ala Val Phe Asn Pro Ser Thr Ser Asn Gly Tyr
340 345 350 Lys Met Val Gly Gln Phe Gly Gln Arg Asn His Ala Ser Val
Ala Asp 355 360 365 Gly His Ala Pro Ile Phe Lys Asp Leu Phe Asp Leu
Gly Val Leu Lys 370 375 380 Ala Pro Val Gly Trp Gln Arg Val Trp Asp
Asp Ala Gly Ser Gly Lys 385 390 395 400 Ser Lys Asp Tyr Ala Cys Trp
Arg Ala Ile Pro Pro Gln Gly Tyr Arg 405 410 415 Ala Leu Gly Asp Val
Met Met Leu Ala Thr Ser Gly Tyr Asn Pro Pro 420 425 430 Asn Leu Pro
Asp Tyr Val Cys Val His Gln Ser Leu Cys Ala Asp Val 435 440 445 Gln
Thr Leu Gln Asn Arg Val Trp Trp Asp Lys Gly Thr Gly Ala Arg 450 455
460 Lys Asp Ala Ser Leu Trp Gln Pro Gly Ala Ala Gly Ala Val Ala Ser
465 470 475 480 Ser Cys Phe Ala Gly Val Pro Asn Tyr Asn Asn Pro Pro
Asn Ser Gly 485 490 495 Asp Ile Glu Arg Leu Arg Gly Ser Ile Ala Cys
Val Lys Thr Ser Ala 500 505 510 Ile Ala Ser Met Gln Glu Met Lys Ser
Met Leu Ser Gln His Gln Gly 515 520 525 Met Glu Ala Met Met Ser Lys
Leu 530 535 7526PRTArtificial sequenceAXMI-205(trun10) 7Met Ala Ser
Ala Ala Asn Ala Gly Gln Leu Gly Asn Leu Pro Gly Val 1 5 10 15 Thr
Ser Met Gly Met Gly Tyr Asp Val Asn Gly Leu Tyr Ala Ser Pro 20 25
30 Glu Ser Leu Leu Gly Gln Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp
35 40 45 Ser Ile Glu Ile Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser
Met His 50 55 60 Val His Thr Tyr Phe His Ser Asp Phe Lys Gln Asp
Val Ser Lys Glu 65 70 75 80 Ile Glu Glu Tyr Arg Glu Lys Met Ser Gln
His Val Gly Val Ser Gly 85 90 95 Arg Tyr Lys Leu Phe Ser Ala Ser
Leu Ser Val Asp Phe Thr Thr Thr 100 105 110 Asp Gln Gln Leu Thr Glu
Ile Thr Tyr Ser Ser Thr Arg Glu Ala His 115 120 125 Val Leu Trp Tyr
Ile Ser Leu Pro Gly Ala Ala Thr Leu Arg Ser Met 130 135 140 Leu Arg
Arg Asp Phe Arg Asp Asp Leu Asn Asn Pro Asn Met Pro Ala 145 150 155
160 Met Glu Leu Phe Lys Arg Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala
165 170 175 Val Gly Gly Arg Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu
Lys Met 180 185 190 Asp Ser Ser Gln Ser Leu Ser Thr Thr Ala Glu Met
Ser Tyr Lys Ala 195 200 205 Leu Val Gly Glu Ile Lys Ile Glu His Gly
Ser Glu Met Glu Lys Gln 210 215 220 Val Asn Ser Phe Arg Ser Asn Ser
Thr Ile Arg Leu Thr Ala Thr Gly 225 230 235 240 Gly Lys Pro Gly Met
Thr Asp Arg Ile Leu His Gly Pro Asp Ser Gln 245 250 255 Gln Ala Phe
Ser Gln Trp Ala Glu Ser Leu Leu Asp Tyr Ala Thr Leu 260 265 270 Met
Asp Phe Ser Thr Glu Ser Leu Gln Pro Ile Trp Ala Leu Ala Asp 275 280
285 Lys Pro Glu Arg Arg Val Glu Leu Glu Asp Ala Phe Pro Glu Phe Met
290 295 300 Lys Gln Ser Gln Gln Ser Ile Pro Lys Val Asp Lys Val Leu
Leu Met 305 310 315 320 Asp Ala Arg Pro Pro Met Val Lys Ala Gly Glu
Asp Ser Gly Ser Gly 325 330 335 Ala Ser Glu Asp Leu Ala Val Phe Asn
Pro Ser Thr Ser Asn Gly Tyr 340 345 350 Lys Met Val Gly Gln Phe Gly
Gln Arg Asn His Ala Ser Val Ala Asp 355 360 365 Gly His Ala Pro Ile
Phe Lys Asp Leu Phe Asp Leu Gly Val Leu Lys 370 375 380 Ala Pro Val
Gly Trp Gln Arg Val Trp Asp Asp Ala Gly Ser Gly Lys 385 390 395 400
Ser Lys Asp Tyr Ala Cys Trp Arg Ala Ile Pro Pro Gln Gly Tyr Arg 405
410 415 Ala Leu Gly Asp Val Met Met Leu Ala Thr Ser Gly Tyr Asn Pro
Pro 420 425 430 Asn Leu Pro Asp Tyr Val Cys Val His Gln Ser Leu Cys
Ala Asp Val 435 440 445 Gln Thr Leu Gln Asn Arg Val Trp Trp Asp Lys
Gly Thr Gly Ala Arg 450 455 460 Lys Asp Val Ser Leu Trp Gln Pro Gly
Ala Ala Gly Ala Val Ala Ser 465 470 475 480 Ser Cys Phe Ala Gly Val
Pro Asn Tyr Asn Asn Pro Pro Asn Ser Gly 485 490 495 Asp Ile Glu Arg
Leu Arg Gly Ser Ile Ala Cys Val Lys Thr Ser Ala 500 505 510 Ile Ala
Ser Met Gln Glu Met Lys Ser Met Leu Ser Gln His 515 520 525
8516PRTArtificial sequenceAXMI-205(trun20) 8Met Ala Ser Ala Ala Asn
Ala Gly Gln Leu Gly Asn Leu Pro Gly Val 1 5 10 15 Thr Ser Met Gly
Met Gly Tyr Asp Val Asn Gly Leu Tyr Ala Ser Pro 20 25 30 Glu Ser
Leu Leu Gly Gln Pro Leu Phe Asp Phe Gly Gly Glu Leu Asp 35 40 45
Ser Ile Glu Ile Glu Gly Arg Ser Tyr Thr Phe Pro Arg Ser Met His 50
55 60 Val His Thr Tyr Phe His Ser Asp Phe Lys Gln Asp Val Ser Lys
Glu 65 70 75 80 Ile Glu Glu Tyr Arg Glu Lys Met Ser Gln His Val Gly
Val Ser Gly 85 90 95 Arg Tyr Lys Leu Phe Ser Ala Ser Leu Ser Val
Asp Phe Thr Thr Thr 100 105 110 Asp Gln Gln Leu Thr Glu Ile Thr Tyr
Ser Ser Thr Arg Glu Ala His 115 120 125 Val Leu Trp Tyr Ile Ser Leu
Pro Gly Ala Ala Thr Leu Arg Ser Met 130 135 140 Leu Arg Arg Asp Phe
Arg Asp Asp Leu Asn Asn Pro Asn Met Pro Ala 145 150 155 160 Met Glu
Leu Phe Lys Arg Tyr Gly Pro Tyr Tyr Ile Ser Glu Ala Ala 165 170 175
Val Gly Gly Arg Leu Asp Tyr Ser Ala Ala Ser Lys Thr Leu Lys Met 180
185 190 Asp Ser Ser Gln Ser Leu Ser Thr Thr Ala Glu Met Ser Tyr Lys
Ala 195 200 205 Leu Val Gly Glu Ile Lys Ile Glu His Gly Ser Glu Met
Glu Lys Gln 210 215 220 Val Asn Ser Phe Arg Ser Asn Ser Thr Ile Arg
Leu Thr Ala Thr Gly 225 230 235 240 Gly Lys Pro Gly Met Thr Asp Arg
Ile Leu His Gly Pro Asp Ser Gln 245 250 255 Gln Ala Phe Ser Gln Trp
Ala Glu Ser Leu Leu Asp Tyr Ala Thr Leu 260 265 270 Met Asp Phe Ser
Thr Glu Ser Leu Gln Pro Ile Trp Ala Leu Ala Asp 275 280 285 Lys Pro
Glu Arg Arg Val Glu Leu Glu Asp Ala Phe Pro Glu Phe Met 290 295 300
Lys Gln Ser Gln Gln Ser Ile Pro Lys Val Asp Lys Val Leu Leu Met 305
310 315 320 Asp Ala Arg Pro Pro Met Val Lys Ala Gly Glu Asp Ser Gly
Ser Gly 325 330 335 Ala Ser Glu Asp Leu Ala Val Phe Asn Pro Ser Thr
Ser Asn Gly Tyr 340 345 350 Lys Met Val Gly Gln Phe Gly Gln Arg Asn
His Ala Ser Val Ala Asp 355 360 365 Gly His Ala Pro Ile Phe Lys Asp
Leu Phe Asp Leu Gly Val Leu Lys 370 375 380 Ala Pro Val Gly Trp Gln
Arg Val Trp Asp Asp Ala Gly Ser Gly Lys 385 390 395 400 Ser Lys Asp
Tyr Ala Cys Trp Arg Ala Ile Pro Pro Gln Gly Tyr Arg 405 410 415 Ala
Leu Gly Asp Val Met Met Leu Ala Thr Ser Gly Tyr Asn Pro Pro 420 425
430 Asn Leu Pro Asp Tyr Val Cys Val His Gln Ser Leu Cys Ala Asp Val
435 440 445 Gln Thr Leu Gln Asn Arg Val Trp Trp Asp Lys Gly Thr Gly
Ala Arg 450 455 460 Lys Asp Val Ser Leu Trp Gln Pro Gly Ala Ala Gly
Ala Val Ala Ser 465 470 475 480 Ser Cys Phe Ala Gly Val Pro Asn Tyr
Asn Asn Pro Pro Asn Ser Gly 485 490 495 Asp Ile Glu Arg Leu Arg Gly
Ser Ile Ala Cys Val Lys Thr Ser Ala 500 505 510 Ile Ala Ser Met 515
91608DNAArtificial sequencesynthetic sequence encoding AXMI-205
9atggcctccg ccgccaatgc tggccagctg ggcaacctcc ccggcgtcac ctccatgggc
60atgggatatg atgtcaatgg cctctatgct tctccagaga gcttgctggg gcagccgctc
120tttgattttg gaggagagct ggacagcatc gagatagaag gaagaagcta
caccttccca 180agaagcatgc atgttcacac ctacttccat tcagatttca
agcaagatgt cagcaaggag 240atcgaggagt acagggagaa gatgagccag
catgttggag tttctggaag atacaagctc 300ttctccgcct ccctctccgt
ggacttcacc accactgatc agcagctgac agagatcacc 360tacagctcaa
caagagaagc tcatgttctc tggtacatct ccctccccgg cgcggccacc
420ttgaggagca tgctgcgccg cgacttcaga gatgatctca acaaccccaa
catgccggcc 480atggagctct tcaagagata tggcccctac tacatctcag
aagctgctgt tggaggaagg 540ctggactaca gcgccgccag caagaccttg
aagatggaca gcagccaaag cctctccacc 600accgccgaga tgagctacaa
ggcgctggtg ggagagatca agattgagca tggatcagag 660atggagaagc
aggtgaacag cttcagaagc aacagcacca tcaggctcac cgccactgga
720ggaaagccag ggatgacaga caggattctt catggacctg acagccagca
ggccttctcc 780caatgggcgg agagcttgct ggattatgcc accttgatgg
acttctcaac agaaagcctc 840cagcccatct gggcgctcgc cgacaagcca
gaaagaaggg tggagctgga ggatgccttc 900cctgagttca tgaagcaaag
tcagcagagc atccccaagg tggacaaggt gctgctgatg 960gatgcaaggc
cgccgatggt gaaggctgga gaagattctg gatctggagc ttcagaagat
1020cttgctgtgt tcaacccctc caccagcaat ggctacaaga tggtgggcca
gtttggccaa 1080aggaaccatg cttctgttgc tgatggccat gctcccatct
tcaaggacct cttcgacctc 1140ggcgtgctga aggctcctgt tggatggcag
cgcgtctggg atgatgctgg atcagggaag 1200agcaaggatt atgcttgctg
gagggccatc cctcctcaag gctacagagc tcttggagat 1260gtcatgatgc
tggccacctc aggctacaac cctccaaatc ttccagatta tgtttgtgtt
1320catcaaagcc tctgtgctga tgttcaaacc ctccagaaca gggtttggtg
ggacaaagga 1380actggagcaa ggaaggatgt cagcttgtgg cagcctggag
ctgctggagc tgtagcaagc 1440agctgctttg ctggagttcc aaactacaac
aaccctccaa actcaggaga cattgagagg 1500ctgagaggaa gcattgcctg
cgtcaagacc tccgccattg cttccatgca agagatgaag 1560agcatgctct
cccagcatca agggatggag gccatgatga gcaagctg 1608101608DNAArtificial
sequencesynthetic sequence encoding AXMI-205 10atggcctctg
ctgccaacgc tggacaactc ggcaacctac caggtgtgac ttccatgggc 60atgggatacg
acgtaaatgg cctttatgct tctcctgaga gcttgctggg gcagccgctc
120tttgattttg gaggagagct ggacagcatc gagatagaag gaagaagcta
caccttccca 180agaagcatgc atgttcacac ctacttccat tcagatttca
agcaagatgt cagcaaggag 240atcgaggagt acagggagaa gatgagccag
catgttggag tttctggaag atacaagctc 300ttctccgcct ccctctccgt
ggacttcacc accactgatc agcagctgac agagatcacc 360tacagctcaa
caagagaagc tcatgttctc tggtacatct ccctccccgg cgcggccacc
420ttgaggagca tgctgcgccg cgacttcaga gatgatctca acaaccccaa
catgccggcc 480atggagctct tcaagagata tggcccctac tacatctcag
aagctgctgt tggaggaagg 540ctggactaca gcgccgccag caagaccttg
aagatggaca gcagccaaag cctctccacc 600accgccgaga tgagctacaa
ggcgctggtg ggagagatca agattgagca tggatcagag 660atggagaagc
aggtgaacag cttcagaagc aacagcacca tcaggctcac cgccactgga
720ggaaagccag ggatgacaga caggattctt catggacctg acagccagca
ggccttctcc 780caatgggcgg agagcttgct ggattatgcc accttgatgg
acttctcaac agaaagcctc 840cagcccatct gggcgctcgc cgacaagcca
gaaagaaggg tggagctgga ggatgccttc 900cctgagttca tgaagcaaag
tcagcagagc atccccaagg tggacaaggt gctgctgatg 960gatgcaaggc
cgccgatggt gaaggctgga gaagattctg gatctggagc ttcagaagat
1020cttgctgtgt tcaacccctc caccagcaat ggctacaaga tggtgggcca
gtttggccaa 1080aggaaccatg cttctgttgc tgatggccat gctcccatct
tcaaggacct cttcgacctc 1140ggcgtgctga aggctcctgt tggatggcag
cgcgtctggg atgatgctgg atcagggaag 1200agcaaggatt atgcttgctg
gagggccatc cctcctcaag gctacagagc tcttggagat 1260gtcatgatgc
tggccacctc aggctacaac cctccaaatc ttccagatta tgtttgtgtt
1320catcaaagcc tctgtgctga tgttcaaacc ctccagaaca gggtttggtg
ggacaaagga 1380actggagcaa ggaaggatgt cagcttgtgg cagcctggag
ctgctggagc tgtagcaagc 1440agctgctttg ctggagttcc aaactacaac
aaccctccaa actcaggaga cattgagagg 1500ctgagaggaa gcattgcctg
cgtcaagacc tccgccattg cttccatgca agagatgaag 1560agcatgctct
cccagcatca agggatggag gccatgatga gcaagctg 1608111608DNAArtificial
sequencesynthetic sequence encoding AXMI-205 11atggcgagtg
ctgccaacgc cgggcagctg ggaaacctgc ccggcgtgac cagcatgggg 60atgggatatg
atgtgaacgg gctctatgcg agcccggaga gcttgctggg gcagccgctc
120tttgattttg gaggagagct ggacagcatc gagatagaag gaagaagcta
caccttccca 180agaagcatgc atgttcacac ctacttccat tcagatttca
agcaagatgt cagcaaggag 240atcgaggagt acagggagaa gatgagccag
catgttggag tttctggaag atacaagctc 300ttctccgcct ccctctccgt
ggacttcacc accactgatc agcagctgac agagatcacc 360tacagctcaa
caagagaagc tcatgttctc tggtacatct ccctccccgg cgcggccacc
420ttgaggagca tgctgcgccg cgacttcaga gatgatctca acaaccccaa
catgccggcc 480atggagctct tcaagagata tggcccctac tacatctcag
aagctgctgt tggaggaagg 540ctggactaca gcgccgccag caagaccttg
aagatggaca gcagccaaag cctctccacc 600accgccgaga tgagctacaa
ggcgctggtg ggagagatca agattgagca tggatcagag 660atggagaagc
aggtgaacag cttcagaagc aacagcacca tcaggctcac cgccactgga
720ggaaagccag ggatgacaga caggattctt catggacctg acagccagca
ggccttctcc 780caatgggcgg agagcttgct
ggattatgcc accttgatgg acttctcaac agaaagcctc 840cagcccatct
gggcgctcgc cgacaagcca gaaagaaggg tggagctgga ggatgccttc
900cctgagttca tgaagcaaag tcagcagagc atccccaagg tggacaaggt
gctgctgatg 960gatgcaaggc cgccgatggt gaaggctgga gaagattctg
gatctggagc ttcagaagat 1020cttgctgtgt tcaacccctc caccagcaat
ggctacaaga tggtgggcca gtttggccaa 1080aggaaccatg cttctgttgc
tgatggccat gctcccatct tcaaggacct cttcgacctc 1140ggcgtgctga
aggctcctgt tggatggcag cgcgtctggg atgatgctgg atcagggaag
1200agcaaggatt atgcttgctg gagggccatc cctcctcaag gctacagagc
tcttggagat 1260gtcatgatgc tggccacctc aggctacaac cctccaaatc
ttccagatta tgtttgtgtt 1320catcaaagcc tctgtgctga tgttcaaacc
ctccagaaca gggtttggtg ggacaaagga 1380actggagcaa ggaaggatgt
cagcttgtgg cagcctggag ctgctggagc tgtagcaagc 1440agctgctttg
ctggagttcc aaactacaac aaccctccaa actcaggaga cattgagagg
1500ctgagaggaa gcattgcctg cgtcaagacc tccgccattg cttccatgca
agagatgaag 1560agcatgctct cccagcatca agggatggag gccatgatga gcaagctg
1608121932DNAArtificial Sequencenucleotide sequence encoding
variant of Axmi-R1 (Axmi-R1(3c7)) 12atgaatccga acaatcgaag
tgaacatgat acaataaaaa ctactgaaaa taatgaggtg 60ccaactaacc atgttcaata
tcctttagcg gaaactccaa atccaacact agaagattta 120aattataaag
agtttttaag aatgactgca gataataata cggaagcact agatagctct
180acaacaaaag atgtcattca aaaaggcatt tccgtagtag gtgatctcct
aggcgtagta 240ggtttcccgt ttggtggagc gcttgtttcg ttttatacaa
actttttaaa tactatttgg 300ccaagtgaag acccgtggaa ggcttttatg
gaacaagtag aagcattgat ggatcagaaa 360atagctgatt atgcaaaaaa
taaagctctt gcagagttac agggccttca aaataatgtc 420gaagattatg
tgagtgcatt gagttcatgg caaaaaaatg caaagagttc acgaaatgta
480catagccagg ggcggataag agagctgttt tctcaagcag aaagtcattt
tcgtaattca 540atgccttcgt ttgcaatttc tggatacgag gttctatttc
taacaacata tgcacaagct 600gccaacacac atttattttt actaaaagac
gctcaaattt atggagaaga atggggatac 660gaaaaagaag atattgctga
attttataaa agacaactaa aacttacgca agaatatact 720gaccattgtg
tcaaatggta taatgttgga ttagataaat taagaggttc atcttatgaa
780tcttgggtaa actttaaccg ttatcgcaga gagatgacat taacagtatt
agatttaatt 840gcactatttc cattgtatga tgttcggcta tacccaaaag
aagttaaaac cgaattaaca 900agagacgttt taacagatcc aattgtcgga
gtcaacaacc ttaggggcta tggaacaacc 960ttctctaata tagaaaatta
tattcgaaaa ccacatctat ttgactatct gcatagaatt 1020caatttcaca
cgcggttcca accaggatat tatggaaatg actctttcaa ttattggtcc
1080ggtaattatg tttcaactag accaagcata ggatcaaatg atataatcac
atctccattc 1140tatggaaata aatccagtga acctgtacaa aatttagaat
ttaatggaga aaaagtctat 1200agagccgtag caaatacaaa tcttgcggtc
tggccgtccg ctgtatattc aggtgttaca 1260aaagtggaat ttagccaata
taatgatcaa acagatgaag caagtacaca aacgtacgac 1320tcaaaaagaa
atgttggcgc ggtcagctgg gattctatcg atcaattgcc tccagaaaca
1380acagatgaac ctctagaaaa gggatatagc catcaactca attatgtaat
gtgcttttta 1440atgcagggta gtagaggaac aatcccagtg ttaacttgga
cacataaaag tgtagacttt 1500tttaacatga ttgattcgaa aaaaattaca
caacttccgt tagtaaaggc atataagtta 1560caatctggtg cttccgttgt
cgcaggtcct aggtttacag gaggagatat cattcaatgc 1620acagaaaatg
gaagtgcggc aactatttac gttacaccgg atgtgtcgta ctctcaaaaa
1680tatcgagcta gaattcatta tgcttctaca tctcagataa catttacact
cagtttagac 1740ggggcaccat ttaatcaata ctatttcgat aaaacgataa
ataaaggaga cacattaacg 1800tataattcat ttaatttagc aagtttcagc
acaccattcg aattatcagg gaataactta 1860caaataggcg tcacaggatt
aagtgctgga gataaagttt atatagacaa aattgaattt 1920attccagtga at
193213644PRTArtificial Sequencevariant of Axmi-R1 (Axmi-R1(3c7))
13Met Asn Pro Asn Asn Arg Ser Glu His Asp Thr Ile Lys Thr Thr Glu 1
5 10 15 Asn Asn Glu Val Pro Thr Asn His Val Gln Tyr Pro Leu Ala Glu
Thr 20 25 30 Pro Asn Pro Thr Leu Glu Asp Leu Asn Tyr Lys Glu Phe
Leu Arg Met 35 40 45 Thr Ala Asp Asn Asn Thr Glu Ala Leu Asp Ser
Ser Thr Thr Lys Asp 50 55 60 Val Ile Gln Lys Gly Ile Ser Val Val
Gly Asp Leu Leu Gly Val Val 65 70 75 80 Gly Phe Pro Phe Gly Gly Ala
Leu Val Ser Phe Tyr Thr Asn Phe Leu 85 90 95 Asn Thr Ile Trp Pro
Ser Glu Asp Pro Trp Lys Ala Phe Met Glu Gln 100 105 110 Val Glu Ala
Leu Met Asp Gln Lys Ile Ala Asp Tyr Ala Lys Asn Lys 115 120 125 Ala
Leu Ala Glu Leu Gln Gly Leu Gln Asn Asn Val Glu Asp Tyr Val 130 135
140 Ser Ala Leu Ser Ser Trp Gln Lys Asn Ala Lys Ser Ser Arg Asn Val
145 150 155 160 His Ser Gln Gly Arg Ile Arg Glu Leu Phe Ser Gln Ala
Glu Ser His 165 170 175 Phe Arg Asn Ser Met Pro Ser Phe Ala Ile Ser
Gly Tyr Glu Val Leu 180 185 190 Phe Leu Thr Thr Tyr Ala Gln Ala Ala
Asn Thr His Leu Phe Leu Leu 195 200 205 Lys Asp Ala Gln Ile Tyr Gly
Glu Glu Trp Gly Tyr Glu Lys Glu Asp 210 215 220 Ile Ala Glu Phe Tyr
Lys Arg Gln Leu Lys Leu Thr Gln Glu Tyr Thr 225 230 235 240 Asp His
Cys Val Lys Trp Tyr Asn Val Gly Leu Asp Lys Leu Arg Gly 245 250 255
Ser Ser Tyr Glu Ser Trp Val Asn Phe Asn Arg Tyr Arg Arg Glu Met 260
265 270 Thr Leu Thr Val Leu Asp Leu Ile Ala Leu Phe Pro Leu Tyr Asp
Val 275 280 285 Arg Leu Tyr Pro Lys Glu Val Lys Thr Glu Leu Thr Arg
Asp Val Leu 290 295 300 Thr Asp Pro Ile Val Gly Val Asn Asn Leu Arg
Gly Tyr Gly Thr Thr 305 310 315 320 Phe Ser Asn Ile Glu Asn Tyr Ile
Arg Lys Pro His Leu Phe Asp Tyr 325 330 335 Leu His Arg Ile Gln Phe
His Thr Arg Phe Gln Pro Gly Tyr Tyr Gly 340 345 350 Asn Asp Ser Phe
Asn Tyr Trp Ser Gly Asn Tyr Val Ser Thr Arg Pro 355 360 365 Ser Ile
Gly Ser Asn Asp Ile Ile Thr Ser Pro Phe Tyr Gly Asn Lys 370 375 380
Ser Ser Glu Pro Val Gln Asn Leu Glu Phe Asn Gly Glu Lys Val Tyr 385
390 395 400 Arg Ala Val Ala Asn Thr Asn Leu Ala Val Trp Pro Ser Ala
Val Tyr 405 410 415 Ser Gly Val Thr Lys Val Glu Phe Ser Gln Tyr Asn
Asp Gln Thr Asp 420 425 430 Glu Ala Ser Thr Gln Thr Tyr Asp Ser Lys
Arg Asn Val Gly Ala Val 435 440 445 Ser Trp Asp Ser Ile Asp Gln Leu
Pro Pro Glu Thr Thr Asp Glu Pro 450 455 460 Leu Glu Lys Gly Tyr Ser
His Gln Leu Asn Tyr Val Met Cys Phe Leu 465 470 475 480 Met Gln Gly
Ser Arg Gly Thr Ile Pro Val Leu Thr Trp Thr His Lys 485 490 495 Ser
Val Asp Phe Phe Asn Met Ile Asp Ser Lys Lys Ile Thr Gln Leu 500 505
510 Pro Leu Val Lys Ala Tyr Lys Leu Gln Ser Gly Ala Ser Val Val Ala
515 520 525 Gly Pro Arg Phe Thr Gly Gly Asp Ile Ile Gln Cys Thr Glu
Asn Gly 530 535 540 Ser Ala Ala Thr Ile Tyr Val Thr Pro Asp Val Ser
Tyr Ser Gln Lys 545 550 555 560 Tyr Arg Ala Arg Ile His Tyr Ala Ser
Thr Ser Gln Ile Thr Phe Thr 565 570 575 Leu Ser Leu Asp Gly Ala Pro
Phe Asn Gln Tyr Tyr Phe Asp Lys Thr 580 585 590 Ile Asn Lys Gly Asp
Thr Leu Thr Tyr Asn Ser Phe Asn Leu Ala Ser 595 600 605 Phe Ser Thr
Pro Phe Glu Leu Ser Gly Asn Asn Leu Gln Ile Gly Val 610 615 620 Thr
Gly Leu Ser Ala Gly Asp Lys Val Tyr Ile Asp Lys Ile Glu Phe 625 630
635 640 Ile Pro Val Asn 141932DNAArtificial Sequencenucleotide
sequence encoding variant of Axmi-R1 (Axmi-R1(1g8)) 14atgaatccga
acaatcgaag tgaacatgat acaataaaaa ctactgaaaa taatgaggtg 60ccaactaacc
atgttcaata tcctttagcg gaaactccaa atccaacact agaagattta
120aattataaag agtttttaag aatgactgca gataataata cggaagcact
agatagctct 180acaacaaaag atgtcattca aaaaggcatt tccgtagtag
gtgatctcct aggcgtagta 240ggtttcccgt ttggtggagc gcttgtttcg
ttttatacaa actttttaaa tactatttgg 300ccaagtgaag acccgtggaa
ggcttttatg gaacaagtag aagcattgat ggatcagaaa 360atagctgatt
atgcaaaaaa taaagctctt gcagagttac agggccttca aaataatgtc
420gaagattatg tgagtgcatt gagttcatgg caaaaaaatc ctgtgagttc
acgaaatcca 480catagccagg ggcggataag agagctgttt tctcaagcag
aaagtcattt tcgtaattca 540atgccttcgt ttgcaatttc tggatacgag
gttctatttc taacaacata tgcacaagct 600gccaacacac atttattttt
actaaaagac gctcaaattt atggagaaga atggggatac 660gaaaaagaag
atattgctga attttataaa agacaactaa aacttacgca agaatatact
720gaccattgtg tcaaatggta taatgttgga ttagataaat taagaggttc
atcttatgaa 780tcttgggtaa actttaaccg ttatcgcaga gagatgacat
taacagtatt agatttaatt 840gcactatttc cattgtatga tgttcggcta
tacccaaaag aagttaaaac cgaattaaca 900agagacgttt taacagatcc
aattgtcgga gtcaacaacc ttagggaata tggaacaacc 960ttctctaata
tagaaaatta tattcgaaaa ccacatctat ttgactatct gcatagaatt
1020caatttcaca cgcggttcca accaggatat tatggaaatg actctttcaa
ttattggtcc 1080ggtaattatg tttcaactag accaagcata ggatcaaatg
atataatcac atctccattc 1140tatggaaata aatccagtga acctgtacaa
aatttagaat ttaatggaga aaaagtctat 1200agagccgtag caaatacaaa
tcttgcggtc tggccgtccg ctgtatattc aggtgttaca 1260aaagtggaat
ttagccaata taatgatcaa acagatgaag caagtacaca aacgtacgac
1320tcaaaaagaa atgttggcgc ggtcagctgg gattctatcg atcaattgcc
tccagaaaca 1380acagatgaac ctctagaaaa gggatatagc catcaactca
attatgtaat gtgcttttta 1440atgctgaaaa gtagaggaac aatcccagtg
ttaacttgga cacataaaag tgtagacttt 1500tttaacatga ttgattcgaa
aaaaattaca caacttccgt tagtaaaggc atataagtta 1560caatctggtg
cttccgttgt cgcaggtcct aggtttacag gaggagatat cattcaatgc
1620acagaaaatg gaagtgcggc aactatttac gttacaccgg atgtgtcgta
ctctcaaaaa 1680tatcgagcta gaattcatta tgcttctaca tctcagataa
catttacact cagtttagac 1740ggggcaccat ttaatcaata ctatttcgat
aaaacgataa ataaaggaga cacattaacg 1800tataattcat ttaatttagc
aagtttcagc acaccattcg aattatcagg gaataactta 1860caaataggcg
tcacaggatt aagtgctgga gataaagttt atatagacaa aattgaattt
1920attccagtga at 193215644PRTArtificial Sequencevariant of Axmi-R1
(Axmi-R1(1g8)) 15Met Asn Pro Asn Asn Arg Ser Glu His Asp Thr Ile
Lys Thr Thr Glu 1 5 10 15 Asn Asn Glu Val Pro Thr Asn His Val Gln
Tyr Pro Leu Ala Glu Thr 20 25 30 Pro Asn Pro Thr Leu Glu Asp Leu
Asn Tyr Lys Glu Phe Leu Arg Met 35 40 45 Thr Ala Asp Asn Asn Thr
Glu Ala Leu Asp Ser Ser Thr Thr Lys Asp 50 55 60 Val Ile Gln Lys
Gly Ile Ser Val Val Gly Asp Leu Leu Gly Val Val 65 70 75 80 Gly Phe
Pro Phe Gly Gly Ala Leu Val Ser Phe Tyr Thr Asn Phe Leu 85 90 95
Asn Thr Ile Trp Pro Ser Glu Asp Pro Trp Lys Ala Phe Met Glu Gln 100
105 110 Val Glu Ala Leu Met Asp Gln Lys Ile Ala Asp Tyr Ala Lys Asn
Lys 115 120 125 Ala Leu Ala Glu Leu Gln Gly Leu Gln Asn Asn Val Glu
Asp Tyr Val 130 135 140 Ser Ala Leu Ser Ser Trp Gln Lys Asn Pro Val
Ser Ser Arg Asn Pro 145 150 155 160 His Ser Gln Gly Arg Ile Arg Glu
Leu Phe Ser Gln Ala Glu Ser His 165 170 175 Phe Arg Asn Ser Met Pro
Ser Phe Ala Ile Ser Gly Tyr Glu Val Leu 180 185 190 Phe Leu Thr Thr
Tyr Ala Gln Ala Ala Asn Thr His Leu Phe Leu Leu 195 200 205 Lys Asp
Ala Gln Ile Tyr Gly Glu Glu Trp Gly Tyr Glu Lys Glu Asp 210 215 220
Ile Ala Glu Phe Tyr Lys Arg Gln Leu Lys Leu Thr Gln Glu Tyr Thr 225
230 235 240 Asp His Cys Val Lys Trp Tyr Asn Val Gly Leu Asp Lys Leu
Arg Gly 245 250 255 Ser Ser Tyr Glu Ser Trp Val Asn Phe Asn Arg Tyr
Arg Arg Glu Met 260 265 270 Thr Leu Thr Val Leu Asp Leu Ile Ala Leu
Phe Pro Leu Tyr Asp Val 275 280 285 Arg Leu Tyr Pro Lys Glu Val Lys
Thr Glu Leu Thr Arg Asp Val Leu 290 295 300 Thr Asp Pro Ile Val Gly
Val Asn Asn Leu Arg Glu Tyr Gly Thr Thr 305 310 315 320 Phe Ser Asn
Ile Glu Asn Tyr Ile Arg Lys Pro His Leu Phe Asp Tyr 325 330 335 Leu
His Arg Ile Gln Phe His Thr Arg Phe Gln Pro Gly Tyr Tyr Gly 340 345
350 Asn Asp Ser Phe Asn Tyr Trp Ser Gly Asn Tyr Val Ser Thr Arg Pro
355 360 365 Ser Ile Gly Ser Asn Asp Ile Ile Thr Ser Pro Phe Tyr Gly
Asn Lys 370 375 380 Ser Ser Glu Pro Val Gln Asn Leu Glu Phe Asn Gly
Glu Lys Val Tyr 385 390 395 400 Arg Ala Val Ala Asn Thr Asn Leu Ala
Val Trp Pro Ser Ala Val Tyr 405 410 415 Ser Gly Val Thr Lys Val Glu
Phe Ser Gln Tyr Asn Asp Gln Thr Asp 420 425 430 Glu Ala Ser Thr Gln
Thr Tyr Asp Ser Lys Arg Asn Val Gly Ala Val 435 440 445 Ser Trp Asp
Ser Ile Asp Gln Leu Pro Pro Glu Thr Thr Asp Glu Pro 450 455 460 Leu
Glu Lys Gly Tyr Ser His Gln Leu Asn Tyr Val Met Cys Phe Leu 465 470
475 480 Met Leu Lys Ser Arg Gly Thr Ile Pro Val Leu Thr Trp Thr His
Lys 485 490 495 Ser Val Asp Phe Phe Asn Met Ile Asp Ser Lys Lys Ile
Thr Gln Leu 500 505 510 Pro Leu Val Lys Ala Tyr Lys Leu Gln Ser Gly
Ala Ser Val Val Ala 515 520 525 Gly Pro Arg Phe Thr Gly Gly Asp Ile
Ile Gln Cys Thr Glu Asn Gly 530 535 540 Ser Ala Ala Thr Ile Tyr Val
Thr Pro Asp Val Ser Tyr Ser Gln Lys 545 550 555 560 Tyr Arg Ala Arg
Ile His Tyr Ala Ser Thr Ser Gln Ile Thr Phe Thr 565 570 575 Leu Ser
Leu Asp Gly Ala Pro Phe Asn Gln Tyr Tyr Phe Asp Lys Thr 580 585 590
Ile Asn Lys Gly Asp Thr Leu Thr Tyr Asn Ser Phe Asn Leu Ala Ser 595
600 605 Phe Ser Thr Pro Phe Glu Leu Ser Gly Asn Asn Leu Gln Ile Gly
Val 610 615 620 Thr Gly Leu Ser Ala Gly Asp Lys Val Tyr Ile Asp Lys
Ile Glu Phe 625 630 635 640 Ile Pro Val Asn 161932DNAArtificial
Sequencenucleotide sequence encoding variant of Axmi-R1
(Axmi-R1(Evo23)) 16atgaatccga acaatcgaag tgaacatgat acaataaaaa
ctactgaaaa taatgaggtg 60ccaactaacc atgttcaata tcctttagcg gaaactccaa
atccaacact agaagattta 120aattataaag agtttttaag aatgactgca
gataataata cggaagcact agatagctct 180acaacaaaag atgtcattca
aaaaggcatt tccgtagtag gtgatctcct aggcgtagta 240ggtttcccgt
ttggtggagc gcttgtttcg ttttatacaa actttttaaa tactatttgg
300ccaagtgaag acccgtggaa ggcttttatg gaacaagtag aagcattgat
ggatcagaaa 360atagctgatt atgcaaaaaa taaagctctt gcagagttac
agggccttca aaataatgtc 420gaagattatg tgagtgcatt gagttcatgg
caaaaaaatc atgtgagttc acgaaatctt 480catagccagg ggcggataag
agagctgttt tctcaagcag aaagtcattt tcgtaattca 540atgccttcgt
ttgcaatttc tggatacgag gttctatttc taacaacata tgcacaagct
600gccaacacac atttattttt actaaaagac gctcaaattt atggagaaga
atggggatac 660gaaaaagaag atattgctga attttataaa agacaactaa
aacttacgca agaatatact 720gaccattgtg tcaaatggta taatgttgga
ttagataaat taagaggttc atcttatgaa 780tcttgggtaa actttaaccg
ttatcgcaga gagatgacat taacagtatt agatttaatt 840gcactatttc
cattgtatga tgttcggcta tacccaaaag aagttaaaac cgaattaaca
900agagacgttt taacagatcc aattgtcgga gtcaacaacc ttagggaata
tggaacaacc 960ttctctaata tagaaaatta tattcgaaaa ccacatctat
ttgactatct gcatagaatt 1020caatttcaca cgcggttcca accaggatat
tatggaaatg actctttcaa ttattggtcc 1080ggtaattatg tttcaactag
accaagcata ggatcaaatg atataatcac atctccattc 1140tatggaaata
aatccagtga acctgtacaa aatttagaat ttaatggaga aaaagtctat
1200agagccgtag caaatacaaa tcttgcggtc tggccgtccg ctgtatattc
aggtgttaca 1260aaagtggaat ttagccaata taatgatcaa acagatgaag
caagtacaca aacgtacgac 1320tcaaaaagaa atgttggcgc ggtcagctgg
gattctatcg atcaattgcc tccagaaaca 1380acagatgaac ctctagaaaa
gggatatagc catcaactca attatgtaat gtgcttttta 1440atgctgaaaa
gtagaggaac aatcccagtg ttaacttgga cacataaaag tgtagacttt
1500tttaacatga ttgattcgaa aaaaattaca
caacttccgt tagtaaaggc atataagtta 1560caatctggtg cttccgttgt
cgcaggtcct aggtttacag gaggagatat cattcaatgc 1620acagaaaatg
gaagtgcggc aactatttac gttacaccgg atgtgtcgta ctctcaaaaa
1680tatcgagcta gaattcatta tgcttctaca tctcagataa catttacact
cagtttagac 1740ggggcaccat ttaatcaata ctatttcgat aaaacgataa
ataaaggaga cacattaacg 1800tataattcat ttaatttagc aagtttcagc
acaccattcg aattatcagg gaataactta 1860caaataggcg tcacaggatt
aagtgctgga gataaagttt atatagacaa aattgaattt 1920attccagtga at
193217644PRTArtificial Sequencevariant of Axmi-R1 (Axmi-R1(Evo23))
17Met Asn Pro Asn Asn Arg Ser Glu His Asp Thr Ile Lys Thr Thr Glu 1
5 10 15 Asn Asn Glu Val Pro Thr Asn His Val Gln Tyr Pro Leu Ala Glu
Thr 20 25 30 Pro Asn Pro Thr Leu Glu Asp Leu Asn Tyr Lys Glu Phe
Leu Arg Met 35 40 45 Thr Ala Asp Asn Asn Thr Glu Ala Leu Asp Ser
Ser Thr Thr Lys Asp 50 55 60 Val Ile Gln Lys Gly Ile Ser Val Val
Gly Asp Leu Leu Gly Val Val 65 70 75 80 Gly Phe Pro Phe Gly Gly Ala
Leu Val Ser Phe Tyr Thr Asn Phe Leu 85 90 95 Asn Thr Ile Trp Pro
Ser Glu Asp Pro Trp Lys Ala Phe Met Glu Gln 100 105 110 Val Glu Ala
Leu Met Asp Gln Lys Ile Ala Asp Tyr Ala Lys Asn Lys 115 120 125 Ala
Leu Ala Glu Leu Gln Gly Leu Gln Asn Asn Val Glu Asp Tyr Val 130 135
140 Ser Ala Leu Ser Ser Trp Gln Lys Asn His Val Ser Ser Arg Asn Leu
145 150 155 160 His Ser Gln Gly Arg Ile Arg Glu Leu Phe Ser Gln Ala
Glu Ser His 165 170 175 Phe Arg Asn Ser Met Pro Ser Phe Ala Ile Ser
Gly Tyr Glu Val Leu 180 185 190 Phe Leu Thr Thr Tyr Ala Gln Ala Ala
Asn Thr His Leu Phe Leu Leu 195 200 205 Lys Asp Ala Gln Ile Tyr Gly
Glu Glu Trp Gly Tyr Glu Lys Glu Asp 210 215 220 Ile Ala Glu Phe Tyr
Lys Arg Gln Leu Lys Leu Thr Gln Glu Tyr Thr 225 230 235 240 Asp His
Cys Val Lys Trp Tyr Asn Val Gly Leu Asp Lys Leu Arg Gly 245 250 255
Ser Ser Tyr Glu Ser Trp Val Asn Phe Asn Arg Tyr Arg Arg Glu Met 260
265 270 Thr Leu Thr Val Leu Asp Leu Ile Ala Leu Phe Pro Leu Tyr Asp
Val 275 280 285 Arg Leu Tyr Pro Lys Glu Val Lys Thr Glu Leu Thr Arg
Asp Val Leu 290 295 300 Thr Asp Pro Ile Val Gly Val Asn Asn Leu Arg
Glu Tyr Gly Thr Thr 305 310 315 320 Phe Ser Asn Ile Glu Asn Tyr Ile
Arg Lys Pro His Leu Phe Asp Tyr 325 330 335 Leu His Arg Ile Gln Phe
His Thr Arg Phe Gln Pro Gly Tyr Tyr Gly 340 345 350 Asn Asp Ser Phe
Asn Tyr Trp Ser Gly Asn Tyr Val Ser Thr Arg Pro 355 360 365 Ser Ile
Gly Ser Asn Asp Ile Ile Thr Ser Pro Phe Tyr Gly Asn Lys 370 375 380
Ser Ser Glu Pro Val Gln Asn Leu Glu Phe Asn Gly Glu Lys Val Tyr 385
390 395 400 Arg Ala Val Ala Asn Thr Asn Leu Ala Val Trp Pro Ser Ala
Val Tyr 405 410 415 Ser Gly Val Thr Lys Val Glu Phe Ser Gln Tyr Asn
Asp Gln Thr Asp 420 425 430 Glu Ala Ser Thr Gln Thr Tyr Asp Ser Lys
Arg Asn Val Gly Ala Val 435 440 445 Ser Trp Asp Ser Ile Asp Gln Leu
Pro Pro Glu Thr Thr Asp Glu Pro 450 455 460 Leu Glu Lys Gly Tyr Ser
His Gln Leu Asn Tyr Val Met Cys Phe Leu 465 470 475 480 Met Leu Lys
Ser Arg Gly Thr Ile Pro Val Leu Thr Trp Thr His Lys 485 490 495 Ser
Val Asp Phe Phe Asn Met Ile Asp Ser Lys Lys Ile Thr Gln Leu 500 505
510 Pro Leu Val Lys Ala Tyr Lys Leu Gln Ser Gly Ala Ser Val Val Ala
515 520 525 Gly Pro Arg Phe Thr Gly Gly Asp Ile Ile Gln Cys Thr Glu
Asn Gly 530 535 540 Ser Ala Ala Thr Ile Tyr Val Thr Pro Asp Val Ser
Tyr Ser Gln Lys 545 550 555 560 Tyr Arg Ala Arg Ile His Tyr Ala Ser
Thr Ser Gln Ile Thr Phe Thr 565 570 575 Leu Ser Leu Asp Gly Ala Pro
Phe Asn Gln Tyr Tyr Phe Asp Lys Thr 580 585 590 Ile Asn Lys Gly Asp
Thr Leu Thr Tyr Asn Ser Phe Asn Leu Ala Ser 595 600 605 Phe Ser Thr
Pro Phe Glu Leu Ser Gly Asn Asn Leu Gln Ile Gly Val 610 615 620 Thr
Gly Leu Ser Ala Gly Asp Lys Val Tyr Ile Asp Lys Ile Glu Phe 625 630
635 640 Ile Pro Val Asn 181932DNAArtificial Sequencenucleotide
sequence encoding variant of Axmi-R1 (Axmi-R1(L61E11))
(Axmi-R1(L61E11)) (Axmi-R1(Evo23)) 18atgaatccga acaatcgaag
tgaacatgat acaataaaaa ctactgaaaa taatgaggtg 60ccaactaacc atgttcaata
tcctttagcg gaaactccaa atccaacact agaagattta 120aattataaag
agtttttaag aatgactgca gataataata cggaagcact agatagctct
180acaacaaaag atgtcattca aaaaggcatt tccgtagtag gtgatctcct
aggcgtagta 240ggtttcccgt ttggtggagc gcttgtttcg ttttatacaa
actttttaaa tactatttgg 300ccaagtgaag acccgtggaa ggcttttatg
gaacaagtag aagcattgat ggatcagaaa 360atagctgatt atgcaaaaaa
taaagctctt gcagagttac agggccttca aaataatgtc 420gaagattatg
tgagtgcatt gagttcatgg caaaaaaatc ctgtgagttc acgaaatcca
480catagccagg ggcggataag agagctgttt tctcaagcag aaagtcattt
tcgtaattca 540atgccttcgt ttgcaatttc tggatacgag gttctatttc
taacaacata tgcacaagct 600gccaacacac atttattttt actaaaagac
gctcaaattt atggagaaga atggggatac 660gaaaaagaag atattgctga
attttataaa agacaactaa aacttacgca agaatatact 720gaccattgtg
tcaaatggta taatgttgga ttagataaat taagaggttc atcttatgaa
780tcttgggtaa actttaaccg ttatcgcaga gagatgacat taacagtatt
agatttaatt 840gcactatttc cattgtatga tgttcggcta tacccaaaag
aagttaaaac cgaattaaca 900agagacgttt taacagatcc aattgtcgga
gtcaacaacc ttaggggcta tggaacaacc 960ttctctaata tagaaaatta
tattcgaaaa ccacatctat ttgactatct gcatagaatt 1020caatttcaca
cgcggttcca accaggatat tatggaaatg actctttcaa ttattggtcc
1080ggtaattatg tttcaactag accaagcata ggatcaaatg atataatcac
atctccattc 1140tatggaaata aatccagtga acctgtacaa aatttagaat
ttaatggaga aaaagtctat 1200agagccgtag caaatacaaa tcttgcggtc
tggccgtccg ctgtatattc aggtgttaca 1260aaagtggaat ttagccaata
taatgatcaa acagatgaag caagtacaca aacgtacgac 1320tcaaaaagaa
atgttggcgc ggtcagctgg gattctatcg atcaattgcc tccagaaaca
1380acagatgaac ctctagaaaa gggatatagc catcaactca attatgtaat
gtgcttttta 1440atgcagggta gtagaggaac aatcccagtg ttaacttgga
cacataaaag tgtagacttt 1500tttaacatga ttgattcgaa aaaaattaca
caacttccgt tagtaaaggc atataattta 1560caatctggtg cttccgttgt
cgcaggtcct aggtttacag gaggagatat cattcaatgc 1620acagaaaatg
gaagtgcggc aactatttac gttacaccgg atgtgtcgta ctctcaaaaa
1680tatcgagcta gaattcatta tgcttctaca tctcagataa catttacact
cagtttagac 1740ggggcaccat ttaatcaata ctatttcgat aaaacgataa
ataaaggaga cacattaacg 1800tataattcat ttaatttagc aagtttcagc
acaccattcg aattatcagg gaataactta 1860caaataggcg tcacaggatt
aagtgctgga gataaagttt atatagacaa aattgaattt 1920attccagtga at
193219644PRTArtificial Sequencevariant of Axmi-R1 (Axmi-R1(L61E11))
19Met Asn Pro Asn Asn Arg Ser Glu His Asp Thr Ile Lys Thr Thr Glu 1
5 10 15 Asn Asn Glu Val Pro Thr Asn His Val Gln Tyr Pro Leu Ala Glu
Thr 20 25 30 Pro Asn Pro Thr Leu Glu Asp Leu Asn Tyr Lys Glu Phe
Leu Arg Met 35 40 45 Thr Ala Asp Asn Asn Thr Glu Ala Leu Asp Ser
Ser Thr Thr Lys Asp 50 55 60 Val Ile Gln Lys Gly Ile Ser Val Val
Gly Asp Leu Leu Gly Val Val 65 70 75 80 Gly Phe Pro Phe Gly Gly Ala
Leu Val Ser Phe Tyr Thr Asn Phe Leu 85 90 95 Asn Thr Ile Trp Pro
Ser Glu Asp Pro Trp Lys Ala Phe Met Glu Gln 100 105 110 Val Glu Ala
Leu Met Asp Gln Lys Ile Ala Asp Tyr Ala Lys Asn Lys 115 120 125 Ala
Leu Ala Glu Leu Gln Gly Leu Gln Asn Asn Val Glu Asp Tyr Val 130 135
140 Ser Ala Leu Ser Ser Trp Gln Lys Asn Pro Val Ser Ser Arg Asn Pro
145 150 155 160 His Ser Gln Gly Arg Ile Arg Glu Leu Phe Ser Gln Ala
Glu Ser His 165 170 175 Phe Arg Asn Ser Met Pro Ser Phe Ala Ile Ser
Gly Tyr Glu Val Leu 180 185 190 Phe Leu Thr Thr Tyr Ala Gln Ala Ala
Asn Thr His Leu Phe Leu Leu 195 200 205 Lys Asp Ala Gln Ile Tyr Gly
Glu Glu Trp Gly Tyr Glu Lys Glu Asp 210 215 220 Ile Ala Glu Phe Tyr
Lys Arg Gln Leu Lys Leu Thr Gln Glu Tyr Thr 225 230 235 240 Asp His
Cys Val Lys Trp Tyr Asn Val Gly Leu Asp Lys Leu Arg Gly 245 250 255
Ser Ser Tyr Glu Ser Trp Val Asn Phe Asn Arg Tyr Arg Arg Glu Met 260
265 270 Thr Leu Thr Val Leu Asp Leu Ile Ala Leu Phe Pro Leu Tyr Asp
Val 275 280 285 Arg Leu Tyr Pro Lys Glu Val Lys Thr Glu Leu Thr Arg
Asp Val Leu 290 295 300 Thr Asp Pro Ile Val Gly Val Asn Asn Leu Arg
Gly Tyr Gly Thr Thr 305 310 315 320 Phe Ser Asn Ile Glu Asn Tyr Ile
Arg Lys Pro His Leu Phe Asp Tyr 325 330 335 Leu His Arg Ile Gln Phe
His Thr Arg Phe Gln Pro Gly Tyr Tyr Gly 340 345 350 Asn Asp Ser Phe
Asn Tyr Trp Ser Gly Asn Tyr Val Ser Thr Arg Pro 355 360 365 Ser Ile
Gly Ser Asn Asp Ile Ile Thr Ser Pro Phe Tyr Gly Asn Lys 370 375 380
Ser Ser Glu Pro Val Gln Asn Leu Glu Phe Asn Gly Glu Lys Val Tyr 385
390 395 400 Arg Ala Val Ala Asn Thr Asn Leu Ala Val Trp Pro Ser Ala
Val Tyr 405 410 415 Ser Gly Val Thr Lys Val Glu Phe Ser Gln Tyr Asn
Asp Gln Thr Asp 420 425 430 Glu Ala Ser Thr Gln Thr Tyr Asp Ser Lys
Arg Asn Val Gly Ala Val 435 440 445 Ser Trp Asp Ser Ile Asp Gln Leu
Pro Pro Glu Thr Thr Asp Glu Pro 450 455 460 Leu Glu Lys Gly Tyr Ser
His Gln Leu Asn Tyr Val Met Cys Phe Leu 465 470 475 480 Met Gln Gly
Ser Arg Gly Thr Ile Pro Val Leu Thr Trp Thr His Lys 485 490 495 Ser
Val Asp Phe Phe Asn Met Ile Asp Ser Lys Lys Ile Thr Gln Leu 500 505
510 Pro Leu Val Lys Ala Tyr Asn Leu Gln Ser Gly Ala Ser Val Val Ala
515 520 525 Gly Pro Arg Phe Thr Gly Gly Asp Ile Ile Gln Cys Thr Glu
Asn Gly 530 535 540 Ser Ala Ala Thr Ile Tyr Val Thr Pro Asp Val Ser
Tyr Ser Gln Lys 545 550 555 560 Tyr Arg Ala Arg Ile His Tyr Ala Ser
Thr Ser Gln Ile Thr Phe Thr 565 570 575 Leu Ser Leu Asp Gly Ala Pro
Phe Asn Gln Tyr Tyr Phe Asp Lys Thr 580 585 590 Ile Asn Lys Gly Asp
Thr Leu Thr Tyr Asn Ser Phe Asn Leu Ala Ser 595 600 605 Phe Ser Thr
Pro Phe Glu Leu Ser Gly Asn Asn Leu Gln Ile Gly Val 610 615 620 Thr
Gly Leu Ser Ala Gly Asp Lys Val Tyr Ile Asp Lys Ile Glu Phe 625 630
635 640 Ile Pro Val Asn 202409DNAArtificial Sequencesynthetic
sequence encoding AXMI-115 20atgaacatga acaacaccaa gctcaatgca
agagctcttc cttccttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc
aaggacatca tgaacatgat cttcaagaca 120gacactggag gagatctcac
cttggatgag atcctcaaga accagcagct gctgaatgag 180atctcaggga
agctggatgg cgtcaatgga agcctcaatg atctcattgc tcaaggaaac
240ctcaacacag agctctccaa ggagatcctc aagatcgcca atgagcagaa
ccaggtgctg 300aatgatgtca acaacaagct ggatgccatc aacaccatgc
tgaacatcta cctccccaag 360atcacctcaa tgctctctga tgtgatgaag
cagaactatg ctctctccct ccagatcgag 420tacctctcaa ggcagctgca
agagatctcc gacaagctgg atgtcatcaa cctcaatgtg 480ctgatcaaca
gcaccttgac agagatcacc ccaagctacc agaggatcaa atatgtcaat
540gagaagttcg acaagctcac cttcgccaca gaatcaaccc tccgcgccaa
gcaaggcatc 600ttcaatgagg acagcttcga caacaacacc ttggagaact
tgacagatct tgctgagctg 660gccaagagca tcaccaagaa tgatgtggac
agcttcgagt tctacctcca caccttccat 720gatgtgctga ttggcaacaa
cctctttgga agaagcgcgc tcaagacagc ttcagagctc 780atcaccaagg
atgagatcaa gacatctgga tcagagattg gcaaggtgta cagcttcctc
840atcgtcctca ccagcctcca ggccaaggcc ttcctcaccc tcaccacctg
ccggaagctg 900ctgggcctct ccgacatcga ctacacctcc atcatgaatg
agcacctcaa caatgagaag 960aatgagttca gagacaacat cctgccggcg
ctctccaaca agttcagcaa cccttcatat 1020gcaaaaacca tcggcagcga
caactacgcc aaggtgatcc tggagagcga gcctggatat 1080gctctggtgg
gcttcgagat catcaatgat cccatccccg tgctgaaggc ctacaaggcc
1140aagctgaagc agaactacca ggtggacaac cagagcctct cagagatcgt
ctacctggac 1200atcgacaagc tcttctgccc agagaacagc gagcagaagt
actacaccaa gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc
accttcgaga agaagctcaa caacctcatc 1320tatgaagcca ccgccaactt
ctatgatcca tcaactggag acatcgacct gaacaagaag 1380caggtggaga
gcaccttccc tcaaacagac tacatcacca tggacattgg agatgatgat
1440ggcatctaca tgccgctcgg cgtcatctca gaaaccttcc tcacccccat
caacagcttc 1500ggcctggagg tggatgccaa gagcaagacc ctcaccttga
aatgcaagag ctacctcagg 1560gagtacctgc tggagagtga tctgaagaac
aaggaaacag ggctgatcgc gccgccaaat 1620gttttcatca gcaatgtggt
gaagaactgg gacattgaag aagattcatt ggagccatgg 1680gtggccaaca
acaagaatgc ttatgtggac aacaccggcg gcattgaaag aagcaaggcg
1740ctcttcaccc aaggagatgg agagttctca cagttcatcg gcgacaagct
gaagccaaac 1800accgactaca tcatccagta caccgtcaag ggcaagccgg
ccatctacct caagaacaag 1860agcaccggct acatcacata tgaggacacc
aatggcaaca gcgaggagtt ccaaaccatt 1920gctgtgaagt tcacctcaga
aacagatctc tcccaaaccc acctggtgtt caagagccaa 1980aatggatatg
aagcatgggg agacaacttc atcatcctgg aggccaagct ctttgaaaca
2040ccagaatctc cagagctcat caagttcaat gattgggaga gatttggcac
cacctacatc 2100actggaaatg agctgaggat tgatcattca agaggaggct
acttccgcca gagcttgaac 2160atcgacagct acagcacata tgatctctcc
ttctccttct ccggcctctg ggccaaggtc 2220atcgtcaaga acagcagagg
agtggtgctc ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca
tctcagagag cttcaccacc gccagcaaca aggatggctt cttcatcgag
2340ctcaccgccg agaggacaag cagcaccttc cacagcttca gagacatctc
catcaaggag 2400aagatcgag 240921803PRTBacillus thuringiensis 21Met
Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1 5 10
15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp
20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu
Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Glu
Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn Asp
Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys Glu
Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn Asp
Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu Asn Ile
Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met Lys
Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Arg 130 135 140
Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Val Ile Asn Leu Asn Val 145
150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ser Tyr Gln
Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr Phe
Ala Thr Glu Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe Asn
Glu Asp Ser Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr Asp
Leu Ala Glu Leu Ala Lys Ser Ile 210 215 220
Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His 225
230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu
Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys Thr
Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile Val
Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr Thr
Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr Ser
Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu Phe
Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335 Asn
Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340 345
350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile Ile
355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys Leu
Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu Ile
Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu Asn
Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro Asp
Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu Asn
Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro Ser
Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455 460 Thr
Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465 470
475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr
Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser Lys
Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr Leu
Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile Ala
Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn Trp Asp
Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala Asn Asn
Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg Ser
Lys Ala Leu Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln Phe 580 585 590
Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr 595
600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr Gly
Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu Phe
Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp Leu
Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr Glu
Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu Phe
Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp Trp
Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu Arg
Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710 715
720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly Leu
725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val Val Leu
Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu Asp Ile
Ser Glu Ser Phe 755 760 765 Thr Thr Ala Ser Asn Lys Asp Gly Phe Phe
Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe His Ser
Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
222409DNAArtificial Sequencesynthetic sequence encoding AXMI-115
22atgaacatga acaacaccaa gctcaatgca agagctcttc cttccttcat cgactacttc
60aatggcatct atggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagaca
120gacactggag gagatctcac cttggatgag atcctcaaga accagcagct
gctgaatgag 180atctcaggga agctggatgg cgtcaatgga agcctcaatg
atctcattgc tcaaggaaac 240ctcaacacag agctctccaa ggagatcctc
aagattgcaa atgagcagaa ccaggtgctg 300aatgatgtca acaacaagct
ggatgccatc aacaccatgc tgaacatcta cctccccaag 360atcacctcaa
tgctctctga tgtgatgaag cagaactatg ctctctccct ccagatcgag
420tacctctcaa ggcagctgca agagatctcc gacaagctgg atgtcatcaa
cctcaatgtg 480ctgatcaaca gcaccttgac agagatcacc ccaagctacc
aaaggatcaa atatgtcaat 540gagaagttcg acaagctcac cttcgccaca
gaatcaaccc tccgcgccaa gcaaggcatc 600ttcaatgagg acagcttcga
caacaacacc ttggagaact tgacagatct tgctgagctg 660gccaagagca
tcaccaagaa tgatgtggac agcttcgagt tctacctcca caccttccat
720gatgtgctga ttggcaacaa cctctttgga agaagcgcgc tcaagacagc
ttcagagctc 780atcaccaagg atgagatcaa gacatctgga tcagaaattg
gcaaggtgta cagcttcctc 840atcgtcctca ccagcctcca ggccaaggcc
ttcctcaccc tcaccacctg ccggaagctg 900ctgggcctct ccgacatcga
ctacacctcc atcatgaatg agcacctcaa caatgagaag 960aatgagttca
gagacaacat cctgccggcg ctctccaaca agttcagcaa cccttcatat
1020gcaaaaacca tcggcagcga caactatgcc aaggtgatcc tggagagcga
gcctggatat 1080gctctggtgg gcttcgagat catcaatgat cccatccctg
tgctgaaggc ctacaaggcc 1140aagctgaagc agaactacca ggtggacaac
caaagcctct cagagatcgt ctacctggac 1200atcgacaagc tcttctgccc
agagaacagc gagcagaagt actacaccaa gaacctcacc 1260ttccctgatg
gatatgtcat caccaagatc accttcgaga agaagctcaa caacctcatc
1320tatgaagcca ccgccaactt ctatgatcca tcaactggag acattgatct
gaacaagaag 1380caggtggaga gcaccttccc tcaaacagat tacatcacca
tggacattgg agatgatgat 1440ggcatctaca tgccgctcgg cgtcatctca
gaaaccttcc tcacccccat caacagcttc 1500ggcctggagg tggatgccaa
gagcaagacc ctcaccttga aatgcaagag ctacttgagg 1560gagtacctgc
tggaatcaga tctgaagaac aaggaaacag ggctgatcgc gccgccaaat
1620gttttcatca gcaatgtggt gaagaactgg gacattgaag aagattcatt
ggagccatgg 1680gtggccaaca acaagaatgc ttatgtggac aacaccggcg
gcattgaaag aagcaaggcg 1740ctcttcaccc aaggagatgg agagttctca
cagttcatcg gcgacaagct gaagccaaac 1800accgactaca tcatccagta
caccgtcaag ggcaagccgg ccatctacct caagaacaag 1860agcactggct
acatcacata tgaggacacc aatggcaaca gcgaggagtt ccaaacaatt
1920gctgtgaagt tcacctcaga aacagatctc tcccaaaccc acctggtgtt
caagagccaa 1980aatggatatg aagcatgggg agacaacttc atcatcctgg
aggccaagct ctttgaaaca 2040ccagaatctc cagagctcat caagttcaat
gattgggaaa gatttggcac cacctacatc 2100actggaaatg agctgaggat
tgatcattca agaggaggct acttccgcca gagcttgaac 2160atcgacagct
acagcacata tgatctctcc ttctccttct ccggcctctg ggccaaggtc
2220atcgtcaaga acagcagagg agtggtgctc ttcgagaagg tgaagaacaa
tggaagcagc 2280tatgaggaca tctcagagag cttcaccacc gccagcaaca
aggatggctt cttcatcgag 2340ctcaccgccg agaggacaag cagcaccttc
cacagcttca gagacatctc catcaaggag 2400aagattgaa
2409232415DNAArtificialAxmi115 variant sequence (Axmi115v02,
pAX6307) 23atgaacatga acaacaccaa gctcaatgca agggcgctgc cgagcttcat
cgactacttc 60aatggcatct atggcttcgc caccggcatc aaggacatca tgaacatgat
cttcaagacc 120gacaccggcg gcaacctcac cttggatgag atcctcaaga
accagcagct gctgaatgag 180atctcaggca agctggacgg cgtcaatgga
agcctcaacg acctcattgc tcaaggcaac 240ctcaacaccg agctgagcaa
ggagatcctc aagattgcaa atgagcagaa ccaggtgctg 300aatgatgtca
acaacaagct ggacgccatc aacaccatgc tgcacatcta cctgccaaag
360atcacctcaa tgctctctga tgtgatgaag cagaactacg cgctgagcct
ccagattgag 420tacctctcaa agcagctgca agagatctcc gacaagctgg
acatcatcaa tgtcaatgtg 480ctcatcaaca gcaccttgac agagatcacg
ccggcctacc agaggatcaa gtatgtcaat 540gagaagttcg acaagctcac
cttcgccacc gagagcaccc tccgcgccaa gcaaggcatc 600ttcaatgaag
attcatttga caacaacacc ttggagaact tgacagacct cgccgagctg
660gccaagagca tcaccaagaa tgatgtggac agcttcgagt tctacctcca
caccttccat 720gatgtgctca tcggcaacaa cctctttgga agaagcgcgc
tcaagacggc atcagagctc 780atcaccaagg atgagatcaa gacaagcggc
agcgagatcg gcaaggtcta cagcttcctc 840atcgtgctga catcattgca
agccaaggcc ttcctcacct tgacaacctg ccgcaagttg 900ctgggcctct
ccgacatcga ctacacctcc atcatgaatg agcacctcaa caatgagaag
960aatgagttca gagacaacat cctgccggcg ctgagcaaca agttcagcaa
cccaagctac 1020gccaagacca tcggctcaga caactacgcc aaggtgatcc
tggagagcga gcctggctac 1080gcgctggtgg gcttcgagat catcaatgat
ccaattcctg ttctcaaggc ctacaaggcc 1140aagctgaagc agaactacca
ggtggacaac cagagcttga gcgagatcgt ctacctggac 1200atcgacaagc
tcttctgccc ggagaactca gagcagaagt actacaccaa gaacctcacc
1260ttccctgatg gatatgtcat caccaagatc accttcgaga agaagctgaa
caacctcatc 1320tacgaggcca ccgccaactt ctatgatcca tcaacaggag
acatcgacct caacaagaag 1380caagtggaga gcaccttccc tcaaacagac
tacatcacca tggacattgg agatgatgat 1440ggcatctaca tgccgctcgg
cgtcatctca gaaaccttct tgacgcccat caacagcttc 1500ggcctggagg
tggacgccaa gagcaagacc ttgacgctca agtgcaagag ctacctcagg
1560gagtacctgc tggagagtga tttgaagaac aaggagacag ggctgatcgc
gccgccaaat 1620gtgttcatca gcaatgtggt gaagaactgg gacatcgagg
aggattcatt ggagccatgg 1680gtggccaaca acaagaatgc ttatgtggac
aacaccggcg gcattgaaag aagcaaggcg 1740ctcttcaccc aaggagatgg
agagttcagc cagttcatcg gcgacaagct aaagcccaac 1800accgactaca
tcatccagta caccgtcaag ggcaagccgg ccatctacct caagaacaag
1860agcaccggct acatcaccta cgaggacacc aatggaaatt ctgaggagtt
ccaaacaatt 1920gctgtgaagt tcacctcaga aacagatttg agccagaccc
acctggtgtt caagagccaa 1980aatggatatg aagcatgggg agacaacttc
atcatcctgg aggccaagct cttcgagaca 2040ccagaaagcc cggagctcat
caagttcaat gattgggaga ggttcggcac cacctacatc 2100accggcaatg
agctgaggat tgatcattca agaggaggct acttccgcca aagcctcaac
2160atcgacagct acagcaccta cgacctcagc ttcagcttca gcggcctctg
ggccaaggtg 2220attgtgaaga acagccgcgg cgtggtgctc ttcgagaagg
tgaagaacaa tggaagcagc 2280tatgaggaca tctcagagag cttcaccacc
gccagcaaca aggatggctt cttcatcgag 2340ctcaccgccg agaggacaag
cagcaccttc cacagcttca gagacatcag catcaaggag 2400aagattgaat agtaa
2415242397DNAArtificialAxmi115 variant sequence (pAX6308)
24atgaacatga acaacaccaa gctcaatgca agggcgctgc cgagcttcat cgactacttc
60aatggcatct atggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc
120gacaccggcg gcaacctcac cttggatgag atcctcaaga accagcagct
gctgaatgag 180atctcaggca agctggacgg cgtcaatgga agcctcaacg
acctcattgc tcaaggcaac 240ctcaacaccg agctgagcaa ggagatcctc
aagattgcaa atgagcagaa ccaggtgctg 300aatgatgtca acaacaagct
ggacgccatc aacaccatgc tgcacatcta cctgccaaag 360atcacctcaa
tgctctctga tgtgatgaag cagaactacg cgctgagcct ccagattgag
420tacctctcaa agcagctgca agagatctcc gacaagctgg acatcatcaa
tgtcaatgtg 480ctcatcaaca gcaccttgac agagatcacg ccggcctacc
agaggatcaa gtatgtcaat 540gagaagtttg aggagctcac cttcgccacc
gagacaacat tgaaggtgaa gaaggacagc 600tcgccggcgg acatcctgga
tgagctcacc gagctaacag agctggccaa gagcgtcacc 660aagaatgatg
ttgatggctt cgagttctac ctcaacacct tccatgatgt gatggtgggc
720aacaacctct tcggccgctc ggcgctcaag acggcgtcgg agctgatcgc
caaggagaat 780gtcaagacaa gtggatcaga ggtgggcaat gtctacaact
tcctcatcgt gctgacggcg 840ctgcaagcca aggccttcct caccttgaca
acctgccgca agttgctggg cctctccgac 900atcgactaca cctccatcat
gaatgagcac ctcaacaatg agaagaatga gttcagagac 960aacatcctgc
cggcgctgag caacaagttc agcaacccaa gctacgccaa gaccatcggc
1020tcagacaact acgccaaggt gatcctggag agcgagcctg gctacgcgct
ggtgggcttc 1080gagatcatca atgatccaat tcctgttctc aaggcctaca
aggccaagct gaagcagaac 1140taccaggtgg acaaccagag cttgagcgag
atcgtctacc tggacatcga caagctcttc 1200tgcccggaga actcagagca
gaagtactac accaagaacc tcaccttccc tgatggatat 1260gtcatcacca
agatcacctt cgagaagaag ctgaacaacc tcatctacga ggccaccgcc
1320aacttctatg atccatcaac aggagacatc gacctcaaca agaagcaagt
ggagagcacc 1380ttccctcaaa cagactacat caccatggac attggagatg
atgatggcat ctacatgccg 1440ctcggcgtca tctcagaaac cttcttgacg
cccatcaaca gcttcggcct ggaggtggac 1500gccaagagca agaccttgac
gctcaagtgc aagagctacc tcagggagta cctgctggag 1560agtgatttga
agaacaagga gacagggctg atcgcgccgc caaatgtgtt catcagcaat
1620gtggtgaaga actgggacat cgaggaggat tcattggagc catgggtggc
caacaacaag 1680aatgcttatg tggacaacac cggcggcatt gaaagaagca
aggcgctctt cacccaagga 1740gatggagagt tcagccagtt catcggcgac
aagctaaagc ccaacaccga ctacatcatc 1800cagtacaccg tcaagggcaa
gccggccatc tacctcaaga acaagagcac cggctacatc 1860acctacgagg
acaccaatgg aaattctgag gagttccaaa caattgctgt gaagttcacc
1920tcagaaacag atttgagcca gacccacctg gtgttcaaga gccaaaatgg
atatgaagca 1980tggggagaca acttcatcat cctggaggcc aagctcttcg
agacaccaga aagcccggag 2040ctcatcaagt tcaatgattg ggagaggttc
ggcaccacct acatcaccgg caatgagctg 2100aggattgatc attcaagagg
aggctacttc cgccaaagcc tcaacatcga cagctacagc 2160acctacgacc
tcagcttcag cttcagcggc ctctgggcca aggtgattgt gaagaacagc
2220cgcggcgtgg tgctcttcga gaaggtgaag aacaatggaa gcagctatga
ggacatctca 2280gagagcttca ccaccgccag caacaaggat ggcttcttca
tcgagctcac cgccgagagg 2340acaagcagca ccttccacag cttcagagac
atcagcatca aggagaagat tgaataa 2397252421DNAArtificialAxmi115
variant sequence (pAX6309) 25atggcacatc accaccacca tcacggatcc
accatgaaca tgaacaacac caagctcaat 60gcaagggcgc tgccgagctt catcgactac
ttcaatggca tctatggctt cgccaccggc 120atcaaggaca tcatgaacat
gatcttcaag accgacaccg gcggcaacct caccttggat 180gagatcctca
agaaccagca gctgctgaat gagatctcag gcaagctgga cggcgtcaat
240ggaagcctca acgacctcat tgctcaaggc aacctcaaca ccgagctgag
caaggagatc 300ctcaagattg caaatgagca gaaccaggtg ctgaatgatg
tcaacaacaa gctggacgcc 360atcaacacca tgctgcacat ctacctgcca
aagatcacct caatgctctc tgatgtgatg 420aagcagaact acgcgctgag
cctccagatt gagtacctct caaagcagct gcaagagatc 480tccgacaagc
tggacatcat caatgtcaat gtgctcatca acagcacctt gacagagatc
540acgccggcct accagaggat caagtatgtc aatgagaagt ttgaggagct
caccttcgcc 600accgagacaa cattgaaggt gaagaaggac agctcgccgg
cggacatcct ggatgagctc 660accgagctaa cagagctggc caagagcgtc
accaagaatg atgttgatgg cttcgagttc 720tacctcaaca ccttccatga
tgtgatggtg ggcaacaacc tcttcggccg ctcggcgctc 780aagacggcgt
cggagctgat cgccaaggag aatgtcaaga caagtggatc agaggtgggc
840aatgtctaca acttcctcat cgtgctgacg gcgctgcaag ccaaggcctt
cctcaccttg 900acaacctgcc gcaagttgct gggcctcgcc gacatcgact
acacctccat catgaatgag 960cacctcaaca aggagaagga ggagttccgc
gtcaacatcc tgccaacatt gagcaacacc 1020ttcagcaacc ccaactacgc
caaggtgaag ggctcagatg aagatgccaa gatgattgtg 1080gaggccaagc
ctggccatgc tctggtgggc ttcgagatga gcaacgacag catcaccgtg
1140ctgaaggtct acgaggccaa gctgaagcag aactaccagg tggacaagga
cagcttgtct 1200gaggtgatct acggcgacat ggacaagctg ctatgtccag
atcaaagcga gcagatctac 1260tacaccaaca acatcgtctt tccaaatgaa
tatgtcatca ccaagatcga cttcaccaag 1320aagatgaaaa cattgagata
tgaggtgacg gccaacagct acgacagcag caccggcgag 1380atcgacctca
acaagaagaa ggtggagagc tcagaagctg agtacaggac gctctccgcc
1440aaggatgatg gcgtctacat gccgctcggc gtcatctcag aaaccttctt
gacgcccatc 1500aatggcttcg gcctccaagc tgatgagaac agcaggctca
tcaccttgac ctgcaagagc 1560tacctcaggg agctgctgct ggccaccgac
ctcagcaaca aggagacaaa gctcatcgtg 1620ccgccatcag gcttcatcag
caacatcgtg gagaatggca acctggaagg agagaacctg 1680gagccatgga
tagccaacaa caagaatgct tatgttgatc acaccggcgg cgtcaatgga
1740acaagggcgc tctatgttca caaggatgga ggcttcagcc agttcatcgg
cgacaagctg 1800aagcccaaga cagaatatgt catccagtac accgtcaagg
gcaagccggc catctacctc 1860aagaacaaga gcaccggcta catcacctac
gaggacacca atggaaattc tgaggagttc 1920caaacaattg ctgtgaagtt
cacctcagaa acagatttga gccagaccca cctggtgttc 1980aagagccaaa
atggatatga agcatgggga gacaacttca tcatcctgga ggccaagctc
2040ttcgagacac cagaaagccc ggagctcatc aagttcaatg attgggagag
gttcggcacc 2100acctacatca ccggcaatga gctgaggatt gatcattcaa
gaggaggcta cttccgccaa 2160agcctcaaca tcgacagcta cagcacctac
gacctcagct tcagcttcag cggcctctgg 2220gccaaggtga ttgtgaagaa
cagccgcggc gtggtgctct tcgagaaggt gaagaacaat 2280ggaagcagct
atgaggacat ctcagagagc ttcaccaccg ccagcaacaa ggatggcttc
2340ttcatcgagc tcaccgccga gaggacaagc agcaccttcc acagcttcag
agacatcagc 2400atcaaggaga agattgaata a
2421262412DNAArtificialAxmi115 variant sequence (Axmi115v02(evo27))
26atgaacatga acaacaccaa gctcaatgca agggcgctgc cgagcttcat cgactacttc
60aatggcatct atggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc
120gacaccggcg gcaacctcac cttggatgag atcctcaaga accagcagct
gctgaatgag 180atctcaggca agctggacgg cgtcaatgga agcctcaacg
acctcattgc tcaaggcaac 240ctcaacaccg agctgagcaa ggagatcctc
aagattgcaa atgagcagaa ccaggtgctg 300aatgatgtca acaacaagct
ggacgccatc aacaccatgc tgcacatcta cctgccaaag 360atcacctcaa
tgctctctga tgtgatgaag cagaactacg cgctgagcct ccagattgag
420tacctctcaa agcagctgca agagatctcc gacaagctgg acatcatcaa
tgtcaatgtg 480ctcatcaaca gcaccttgac agagatcacg ccggcctacc
agaggatcaa gtatgtcaat 540gagaagttcg acaagctcac cttcgccacc
gagagcaccc tccgcgccaa gcaaggcatc 600ttcaatgaag attcatttga
caacaacacc ttggagaact tgacagacct cgccgagctg 660gccaagagca
tcaccaagaa tgatgtggac agcttcgagt tctacctcca caccttccat
720gatgtgctca tcggcaacaa cctctttgga agaagcgcgc tcaagacggc
atcagagctc 780atcaccaagg atgagatcaa gacaagcggc agcgagatcg
gcaaggtcta cagcttcctc 840atcgtgctga catcattgca agccaaggcc
ttcctcacct tgacaacctg ccgcaagttg 900ctgggcctct ccgacatcga
ctacacctcc atcatgaatg agcacctcaa caatgagaag 960aatgagttca
gagacaacat cctgccggcg ctgagcaaca agttcagcaa cccaagctac
1020gccaagacca tcggctcaga caactacgcc aaggtgatcc tggagagcga
gcctggctac 1080gcgctggtgg gcttcgagat catcaatgat ccaattcctg
ttctcaaggc ctacaaggcc 1140aagctgaagc agaactacca ggtggacaac
cagagcttga gcgagatcgt ctacctggac
1200atcgacaagc tcttctgccc ggagaactca gagcagaagt actacaccaa
gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc accttcgaga
agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt ctatgatcca
tcaacaggag acatcgacct caacaagaag 1380caagtggaga gcaccttccc
tcaaacagac tacatcacca tggacattgg agatgatgat 1440ggcatctaca
tgccgctcgg cgtcatctca gaaaccttct tgacgcccat caacagcttc
1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca agtgcaagag
ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac aaggagacag
ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt gaagaactgg
gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca acaagaatgc
ttatgtggac aacaccggcg gcattgaaag aagcaaggcg 1740ctcttcaccc
aaggagatgg aaagttcagc cagttcatcg gcgacaagct aaagcccaac
1800accgactaca tcatccagta caccgtcaag ggcaagccgg ccatctacct
caagaacaag 1860agcaccggct acatcaccta cgaggacacc aatggaaatt
ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga aacagatttg
agccagaccc acctggtgtt caagagccaa 1980aatggatatg aagcatgggg
agacaacttc atcatcctgg aggccaagct cttcgagaca 2040ccagaaagcc
cggagctcat caagttcaat gattgggaga ggttcggcac cacctacatc
2100accggcaatg agctgaggat tgatcattca agaggaggct acttccgcca
aagcctcaac 2160atcgacagct acagcaccta cgacctcagc ttcagcttca
gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg cgtggtgctc
ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca tctcagagag
cttcaccacc gccagcaaca aggatggctt cttcatcgag 2340ctcaccgccg
agaggacaag cagcaccttc cacagcttca gagacatcag catcaaggag
2400aagattgaat aa 2412272412DNAArtificialAxmi115 variant sequence
(Axmi115v02(evo28)) 27atgaacatga acaacaccaa gctcaatgca agggcgctgc
cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc aaggacatca
tgaacatgat cttcaagacc 120gacaccggcg gcaacctcac cttggatgag
atcctcaaga accagcagct gctgaatgag 180atctcaggca agctggacgg
cgtcaatgga agcctcaacg acctcattgc tcaaggcaac 240ctcaacaccg
agctgagcaa ggagatcctc aagattgcaa atgagcagaa ccaggtgctg
300aatgatgtca acaacaagct ggacgccatc aacaccatgc tgcacatcta
cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag cagaactacg
cgctgagcct ccagattgag 420tacctctcaa agcagctgca agagatctcc
gacaagctgg acatcatcaa tgtcaatgtg 480ctcatcaaca gcaccttgac
agagatcacg ccggcctacc agaggatcaa gtatgtcaat 540gagaagttcg
acaagctcac cttcgccacc gagagcaccc tccgcgccaa gcaaggcatc
600ttcaatgaag attcatttga caacaacacc ttggagaact tgacagacct
cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac agcttcgagt
tctacctcca caccttccat 720gatgtgctca tcggcaacaa cctctttgga
agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg atgagatcaa
gacaagcggc agcgagatcg gcaaggtcta cagcttcctc 840atcgtgctga
catcattgca agccaaggcc ttcctcacct tgacaacctg ccgcaagttg
900ctgggcctct ccgacatcga ctacacctcc atcatgaatg agcacctcaa
caatgagaag 960aatgagttca gagacaacat cctgccggcg ctgagcaaca
agttcagcaa cccaagctac 1020gccaagacca tcggctcaga caactacgcc
aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg gcttcgagat
catcaatgat ccaattcctg ttctcaaggc ctacaaggcc 1140aagctgaagc
agaactacca ggtggacaac cagagcttga gcgagatcgt ctacctggac
1200atcgacaagc tcttctgccc ggagaactca gagcagaagt actacaccaa
gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc accttcgaga
agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt ctatgatcca
tcaacaggag acatcgacct caacaagaag 1380caagtggaga gcaccttccc
tcaaacagac tacatcacca tggacattgg agatgatgat 1440ggcatctaca
tgccgctcgg cgtcatctca gaaaccttct tgacgcccat caacagcttc
1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca agtgcaagag
ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac aaggagacag
ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt gaagaactgg
gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca acaagaatgc
ttatgtggac aacaccggcg gcattgaaag aagcaaggcg 1740ctcttcaccc
aaggagatgg agagttcagc cagttcatcg gcgacaagct aaagcccaac
1800accgactaca tcatccagta caccgtcaag ggcaagccgg ccatctacct
caagaacaag 1860agcaccggct acatcaccta cgaggacacc aatggaaatt
ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga aacagatttg
agccagaccc acctggtgtt caagagccaa 1980aatggatatg aagcatgggg
agacaacttc atcatcctgg aggccaagct cttcgagaca 2040ccagaaagcc
cggagctcat caagttcaat gattgggaga ggttcggcac cacctacatc
2100accggcaatg agctgaggat tgatcattca agaggaggct acttccgcca
aagcctcaac 2160atcgacagct acagcaccta cgacctcagc ttcagcttca
gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg cgtggtgctc
ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca tctcagagag
cttcaccacc atgagcaaca aggatggctt cttcatcgag 2340ctcaccgccg
agaggacaag cagcaccttc cacagcttca gagacatcag catcaaggag
2400aagattgaat aa 2412282412DNAArtificialAxmi115 variant sequence
(Axmi115v02(evo29)) 28atgaacatga acaacaccaa gctcaatgca agggcgctgc
cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc aaggacatca
tgaacatgat cttcaagacc 120gacaccggcg gcaacctcac cttggatgag
atcctcaaga accagcagct gctgaatgag 180atctcaggca agctggacgg
cgtcaatgga agcctcaacg acctcattgc tcaaggcaac 240ctcaacaccg
agctgagcaa ggagatcctc aagattgcaa atgagcagaa ccaggtgctg
300aatgatgtca acaacaagct ggacgccatc aacaccatgc tgcacatcta
cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag cagaactacg
cgctgagcct ccagattgag 420tacctctcaa agcagctgca agagatctcc
gacaagctgg acatcatcaa tgtcaatgtg 480ctcatcaaca gcaccttgac
agagatcacg ccggcctacc agaggatcaa gtatgtcaat 540gagaagttcg
acaagctcac cttcgccacc gagagcaccc tccgcgccaa gcaaggcatc
600ttcaatgaag attcatttga caacaacacc ttggagaact tgacagacct
cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac agcttcgagt
tctacctcca caccttccat 720gatgtgctca tcggcaacaa cctctttgga
agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg atgagatcaa
gacaagcggc agcgagatcg gcaaggtcta cagcttcctc 840atcgtgctga
catcattgca agccaaggcc ttcctcacct tgacaacctg ccgcaagttg
900ctgggcctct ccgacatcga ctacacctcc atcatgaatg agcacctcaa
caatgagaag 960aatgagttca gagacaacat cctgccggcg ctgagcaaca
agttcagcaa cccaagctac 1020gccaagacca tcggctcaga caactacgcc
aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg gcttcgagat
catcaatgat ccaattcctg ttctcaaggc ctacaaggcc 1140aagctgaagc
agaactacca ggtggacaac cagagcttga gcgagatcgt ctacctggac
1200atcgacaagc tcttctgccc ggagaactca gagcagaagt actacaccaa
gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc accttcgaga
agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt ctatgatcca
tcaacaggag acatcgacct caacaagaag 1380caagtggaga gcaccttccc
tcaaacagac tacatcacca tggacattgg agatgatgat 1440ggcatctaca
tgccgctcgg cgtcatctca gaaaccttct tgacgcccat caacagcttc
1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca agtgcaagag
ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac aaggagacag
ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt gaagaactgg
gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca acaagaatgc
ttatgtggac aacaccggcg gcattgaaag aagcaaggcg 1740ctcttcaccg
taggagatgg agagttcagc cagttcatcg gcgacaagct aaagcccaac
1800accgactaca tcatccagta caccgtcaag ggcaagccgg ccatctacct
caagaacaag 1860agcaccggct acatcaccta cgaggacacc aatggaaatt
ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga aacagatttg
agccagaccc acctggtgtt caagagccaa 1980aatggatatg aagcatgggg
agacaacttc atcatcctgg aggccaagct cttcgagaca 2040ccagaaagcc
cggagctcat caagttcaat gattgggaga ggttcggcac cacctacatc
2100accggcaatg agctgaggat tgatcattca agaggaggct acttccgcca
aagcctcaac 2160atcgacagct acagcaccta cgacctcagc ttcagcttca
gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg cgtggtgctc
ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca tctcagagag
cttcaccacc gccagcaaca aggatggctt cttcatcgag 2340ctcaccgccg
agaggacaag cagcaccttc cacagcttca gagacatcag catcaaggag
2400aagattgaat aa 2412292409DNAArtificialAxmi115 variant sequence
(Axmi115v02(evo31)) 29atgaacatga acaacaccaa gctcaatgca agggcgctgc
cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc aaggacatca
tgaacatgat cttcaagacc 120gacaccggcg gcaacctcac cttggatgag
atcctcaaga accagcagct gctgaatgag 180atctcaggca agctggacgg
cgtcaatgga agcctcaacg acctcattgc tcaaggcaac 240ctcaacaccg
agctgagcaa ggagatcctc aagattgcaa atgagcagaa ccaggtgctg
300aatgatgtca acaacaagct ggacgccatc aacaccatgc tgcacatcta
cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag cagaactacg
cgctgagcct ccagattgag 420tacctctcaa agcagctgca agagatctcc
gacaagctgg acatcatcaa tgtcaatgtg 480ctcatcaaca gcaccttgac
agagatcacg ccggcctacc agaggatcaa gtatgtcaat 540gagaagttcg
acaagctcac cttcgccacc gagagcaccc tccgcgccaa gcaaggcatc
600ttcaatgaag attcatttga caacaacacc ttggagaact tgacagacct
cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac agcttcgagt
tctacctcca caccttccat 720gatgtgctca tcggcaacaa cctctttgga
agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg atgagatcaa
gacaagcggc agcgagatcg gcaaggtcta cagcttcctc 840atcgtgctga
catcattgca agccaaggcc ttcctcacct tgacaacctg ccgcaagttg
900ctgggcctct ccgacatcga ctacacctcc atcatgaatg agcacctcaa
caatgagaag 960aatgagttca gagacaacat cctgccggcg ctgagcaaca
agttcagcaa cccaagctac 1020gccaagacca tcggctcaga caactacgcc
aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg gcttcgagat
catcaatgat ccaattcctg ttctcaaggc ctacaaggcc 1140aagctgaagc
agaactacca ggtggacaac cagagcttga gcgagatcgt ctacctggac
1200atcgacaagc tcttctgccc ggagaactca gagcagaagt actacaccaa
gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc accttcgaga
agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt ctatgatcca
tcaacaggag acatcgacct caacaagaag 1380caagtggaga gcaccttccc
tcaaacagac tacatcacca tggacattgg agatgatgat 1440ggcatctaca
tgccgctcgg cgtcatctca gaaaccttct tgacgcccat caacagcttc
1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca agtgcaagag
ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac aaggagacag
ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt gaagaactgg
gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca acaagaatgc
ttatgtggac aacaccggcg gcattgaaag aagcaaggcg 1740ctcttcaccc
aaggagatgg agagttcagc cagttcatcg gcgacaagct aaagcccaac
1800accgactaca tcatccagta caccgtcaag ggcaagccgg ccatctacct
caagaacaag 1860agcaccggct acatcaccta cgaggacacc aatggaaatt
ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga aacagatttg
agccagaccc acctggtgtt caagagccaa 1980aatggatatg aagcatgggg
agacaacttc atcatcctgg aggccaagct cttcgagaca 2040ccagaaagcc
cggagctcat caagttcaat gattgggaga ggttcggcac cacctacatc
2100accggcaatg agctgaggat tgatcattca agaggaggct acttccgcca
aagcctcaac 2160atcgacagct acagcaccta cgacctcagc ttcagcttca
gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg cgtggtgctc
ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca tctcagagga
cttcaccacc aatggcttta aggatggctt ctatatcgag 2340ctcaccgccg
agaggacaag cagcaccttc cacagcttca gagacatcag catcaaggag
2400aagattgaa 2409302409DNAArtificialAxmi115 variant sequence
(Axmi115v02(evo32)) 30atgaacatga acaacaccaa gctcaatgca agggcgctgc
cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc aaggacatca
tgaacatgat cttcaagacc 120gacaccggcg gcaacctcac cttggatgag
atcctcaaga accagcagct gctgaatgag 180atctcaggca agctggacgg
cgtcaatgga agcctcaacg acctcattgc tcaaggcaac 240ctcaacaccg
agctgagcaa ggagatcctc aagattgcaa atgagcagaa ccaggtgctg
300aatgatgtca acaacaagct ggacgccatc aacaccatgc tgcacatcta
cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag cagaactacg
cgctgagcct ccagattgag 420tacctctcaa agcagctgca agagatctcc
gacaagctgg acatcatcaa tgtcaatgtg 480ctcatcaaca gcaccttgac
agagatcacg ccggcctacc agaggatcaa gtatgtcaat 540gagaagttcg
acaagctcac cttcgccacc gagagcaccc tccgcgccaa gcaaggcatc
600ttcaatgaag attcatttga caacaacacc ttggagaact tgacagacct
cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac agcttcgagt
tctacctcca caccttccat 720gatgtgctca tcggcaacaa cctctttgga
agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg atgagatcaa
gacaagcggc agcgagatcg gcaaggtcta cagcttcctc 840atcgtgctga
catcattgca agccaaggcc ttcctcacct tgacaacctg ccgcaagttg
900ctgggcctct ccgacatcga ctacacctcc atcatgaatg agcacctcaa
caatgagaag 960aatgagttca gagacaacat cctgccggcg ctgagcaaca
agttcagcaa cccaagctac 1020gccaagacca tcggctcaga caactacgcc
aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg gcttcgagat
catcaatgat ccaattcctg ttctcaaggc ctacaaggcc 1140aagctgaagc
agaactacca ggtggacaac cagagcttga gcgagatcgt ctacctggac
1200atcgacaagc tcttctgccc ggagaactca gagcagaagt actacaccaa
gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc accttcgaga
agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt ctatgatcca
tcaacaggag acatcgacct caacaagaag 1380caagtggaga gcaccttccc
tcaaacagac tacatcacca tggacattgg agatgatgat 1440ggcatctaca
tgccgctcgg cgtcatctca gaaaccttct tgacgcccat caacagcttc
1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca agtgcaagag
ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac aaggagacag
ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt gaagaactgg
gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca acaagaatgc
ttatgtggac aacaccggcg gcattgaaag aagcaaggcg 1740ctcttcaccc
aaggagatgg agagttcagc cagttcatcg gcgacaagct aaagcccaac
1800accgactaca tcatccagta caccgtcaag ggcaagccgg ccatctacct
caagaacaag 1860agcaccggct acatcaccta cgaggacacc aatggaaatt
ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga aacagatttg
agccagaccc acctggtgtt caagagccaa 1980aatggatatg aagcatgggg
agacaacttc atcatcctgg aggccaagct cttcgagaca 2040ccagaaagcc
cggagctcat caagttcaat gattgggaga ggttcggcac cacctacatc
2100accggcaatg agctgaggat tgatcattca agaggaggct acttccgcca
aagcctcaac 2160atcgacagct acagcaccta cgacctcagc ttcagcttca
gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg cgtggtgctc
ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca tctcagagca
cttcaccacc tggggctata aggatggctt ctttatcgag 2340ctcaccgccg
agaggacaag cagcaccttc cacagcttca gagacatcag catcaaggag
2400aagattgaa 2409312412DNAArtificialAxmi115 variant sequence
(Axmi115_Block2_L11C7) 31atgaacatga acaacaccaa gctcaatgca
agggcgctgc cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc
aaggacatca tgaacatgat cttcaagacc 120gacaccggcg gcgacctcac
cttggatgag atcctcaaga accagcagct gctgaatgag 180atctcaggca
agctggacgg cgtcaatgga agcctcaacg acctcattgc tcaaggcaac
240ctcaacaccg agctgagcaa ggagatcctc aagattgcaa atgagcagaa
ccaggtgctg 300aatgatgtca acaacaagct ggacgccatc aacaccatgc
tcaacatcta cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag
cagaactacg cgctgagcct ccagattgag 420tacctctcaa ggcagctgca
agagatctcc gacaagctgg atgtcatcaa cctcaatgtg 480ctcatcaaca
gcaccttgac agagatcacg ccaagctacc agaggatcaa gtatgtcaat
540gagaagttcg acaagctcac cttcgccacc gagagcaccc tccgcgccaa
gcaaggcatc 600ttcaatgaag attcatttga caacaacacc ttggagaact
tgacagacct cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac
agcttcgagt tctacctcca caccttccat 720gatgtgctca tcggcaacaa
cctctttgga agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg
atgagatcaa gacaagcggc agcgagatcg gcaaggtcta cagcttcctc
840atcgtgctga catcattgca agccaaggcc ttcctcacct tgacaacctg
ccgcaagttg 900ctgggcctct ccgacatcga ctacacctcc atcatgaatg
agcacctcaa caatgagaag 960aatgagttca gagacaacat cctgccggcg
ctgagcaaca agttcagcaa cccaagctac 1020gccaagacca tcggctcaga
caactacgcc aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg
gcttcgagat catcaatgat ccaattcctg ttctcaaggc ctacaaggcc
1140aagctgaagc agaactacca ggtggacaac cagagcttga gcgagatcgt
ctacctggac 1200atcgacaagc tcttctgccc ggagaactca gagcagaagt
actacaccaa gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc
accttcgaga agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt
ctatgatcca tcaacaggag acatcgacct caacaagaag 1380caagtggaga
gcaccttccc tcaaacagac tacatcacca tggacattgg agatgatgat
1440ggcatctaca tgccgctcgg cgtcatctca gaaaccttct tgacgcccat
caacagcttc 1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca
agtgcaagag ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac
aaggagacag ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt
gaagaactgg gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca
acaagaatgc ttatgtggac aacaccggcg gcattgaaag aagcaaggcg
1740ctcttcgtcc aaggagatgg agagttcagc cagttcatcg gcgacaagct
aaagcccaac 1800accgactaca tcatccagta caccgtcaag ggcaagccgg
ccatctacct caagaacaag 1860agcaccggct acatcaccta cgaggacacc
aatggaaatt ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga
aacagatttg agccagaccc acctggtgtt caagagccaa 1980aatggatatg
aagcatgggg agacaacttc atcatcctgg aggccaagct cttcgagaca
2040ccagaaagcc cggagctcat caagttcaat gattgggaga ggttcggcac
cacctacatc 2100accggcaatg agctgaggat tgatcattca agaggaggct
acttccgcca aagcctcaac 2160atcgacagct acagcaccta cgacctcagc
ttcagcttca gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg
cgtggtgctc ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca
tctcagagag cttcaccacc gccagcaaca aggatggctt cttcatcgag
2340ctcaccgccg agaggacaag cagcaccttc cacagcttca gagacatcag
catcaaggag 2400aagattgaat aa 2412322412DNAArtificialAxmi115 variant
sequence (Axmi115_Block2_L11H7) 32atgaacatga acaacaccaa gctcaatgca
agggcgctgc cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc
aaggacatca tgaacatgat cttcaagacc 120gacaccggcg gcgacctcac
cttggatgag atcctcaaga accagcagct gctgaatgag 180atctcaggca
agctggacgg cgtcaatgga agcctcaacg acctcattgc tcaaggcaac
240ctcaacaccg agctgagcaa ggagatcctc aagattgcaa atgagcagaa
ccaggtgctg 300aatgatgtca acaacaagct ggacgccatc aacaccatgc
tcaacatcta cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag
cagaactacg cgctgagcct ccagattgag 420tacctctcaa ggcagctgca
agagatctcc gacaagctgg atgtcatcaa cctcaatgtg 480ctcatcaaca
gcaccttgac agagatcacg ccaagctacc agaggatcaa gtatgtcaat
540gagaagttcg acaagctcac cttcgccacc gagagcaccc tccgcgccaa
gcaaggcatc 600ttcaatgaag attcatttga caacaacacc ttggagaact
tgacagacct cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac
agcttcgagt tctacctcca caccttccat 720gatgtgctca tcggcaacaa
cctctttgga agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg
atgagatcaa gacaagcggc agcgagatcg gcaaggtcta cagcttcctc
840atcgtgctga catcattgca agccaaggcc ttcctcacct tgacaacctg
ccgcaagttg 900ctgggcctct ccgacatcga ctacacctcc atcatgaatg
agcacctcaa caatgagaag 960aatgagttca gagacaacat cctgccggcg
ctgagcaaca agttcagcaa cccaagctac 1020gccaagacca tcggctcaga
caactacgcc aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg
gcttcgagat catcaatgat ccaattcctg ttctcaaggc ctacaaggcc
1140aagctgaagc agaactacca ggtggacaac cagagcttga gcgagatcgt
ctacctggac 1200atcgacaagc tcttctgccc ggagaactca gagcagaagt
actacaccaa gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc
accttcgaga agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt
ctatgatcca tcaacaggag acatcgacct caacaagaag 1380caagtggaga
gcaccttccc tcaaacagac tacatcacca tggacattgg agatgatgat
1440ggcatctaca tgccgctcgg cgtcatctca gaaaccttct tgacgcccat
caacagcttc 1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca
agtgcaagag ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac
aaggagacag ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt
gaagaactgg gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca
acaagaatgc ttatgtggac aacaccggcg gcattgaaag aagcaaggcg
1740ctcttcaccc ttggagatgg agagttcagc cagttcatcg gcgacaagct
aaagcccaac 1800accgactaca tcatccagta caccgtcaag ggcaagccgg
ccatctacct caagaacaag 1860agcaccggct acatcaccta cgaggacacc
aatggaaatt ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga
aacagatttg agccagaccc acctggtgtt caagagccaa 1980aatggatatg
aagcatgggg agacaacttc atcatcctgg aggccaagct cttcgagaca
2040ccagaaagcc cggagctcat caagttcaat gattgggaga ggttcggcac
cacctacatc 2100accggcaatg agctgaggat tgatcattca agaggaggct
acttccgcca aagcctcaac 2160atcgacagct acagcaccta cgacctcagc
ttcagcttca gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg
cgtggtgctc ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca
tctcagagag cttcaccacc gccagcaaca aggatggctt cttcatcgag
2340ctcaccgccg agaggacaag cagcaccttc cacagcttca gagacatcag
catcaaggag 2400aagattgaat aa 2412332412DNAArtificialAxmi115 variant
sequence (Axmi115_Block2_L11A9) 33atgaacatga acaacaccaa gctcaatgca
agggcgctgc cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc
aaggacatca tgaacatgat cttcaagacc 120gacaccggcg gcgacctcac
cttggatgag atcctcaaga accagcagct gctgaatgag 180atctcaggca
agctggacgg cgtcaatgga agcctcaacg acctcattgc tcaaggcaac
240ctcaacaccg agctgagcaa ggagatcctc aagattgcaa atgagcagaa
ccaggtgctg 300aatgatgtca acaacaagct ggacgccatc aacaccatgc
tcaacatcta cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag
cagaactacg cgctgagcct ccagattgag 420tacctctcaa ggcagctgca
agagatctcc gacaagctgg atgtcatcaa cctcaatgtg 480ctcatcaaca
gcaccttgac agagatcacg ccaagctacc agaggatcaa gtatgtcaat
540gagaagttcg acaagctcac cttcgccacc gagagcaccc tccgcgccaa
gcaaggcatc 600ttcaatgaag attcatttga caacaacacc ttggagaact
tgacagacct cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac
agcttcgagt tctacctcca caccttccat 720gatgtgctca tcggcaacaa
cctctttgga agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg
atgagatcaa gacaagcggc agcgagatcg gcaaggtcta cagcttcctc
840atcgtgctga catcattgca agccaaggcc ttcctcacct tgacaacctg
ccgcaagttg 900ctgggcctct ccgacatcga ctacacctcc atcatgaatg
agcacctcaa caatgagaag 960aatgagttca gagacaacat cctgccggcg
ctgagcaaca agttcagcaa cccaagctac 1020gccaagacca tcggctcaga
caactacgcc aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg
gcttcgagat catcaatgat ccaattcctg ttctcaaggc ctacaaggcc
1140aagctgaagc agaactacca ggtggacaac cagagcttga gcgagatcgt
ctacctggac 1200atcgacaagc tcttctgccc ggagaactca gagcagaagt
actacaccaa gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc
accttcgaga agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt
ctatgatcca tcaacaggag acatcgacct caacaagaag 1380caagtggaga
gcaccttccc tcaaacagac tacatcacca tggacattgg agatgatgat
1440ggcatctaca tgccgctcgg cgtcatctca gaaaccttct tgacgcccat
caacagcttc 1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca
agtgcaagag ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac
aaggagacag ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt
gaagaactgg gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca
acaagaatgc ttatgtggac aacaccggcg gcattgaaag aagcaaggcg
1740ctcttcacca aaggagatgg agagttcagc cagttcatcg gcgacaagct
aaagcccaac 1800accgactaca tcatccagta caccgtcaag ggcaagccgg
ccatctacct caagaacaag 1860agcaccggct acatcaccta cgaggacacc
aatggaaatt ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga
aacagatttg agccagaccc acctggtgtt caagagccaa 1980aatggatatg
aagcatgggg agacaacttc atcatcctgg aggccaagct cttcgagaca
2040ccagaaagcc cggagctcat caagttcaat gattgggaga ggttcggcac
cacctacatc 2100accggcaatg agctgaggat tgatcattca agaggaggct
acttccgcca aagcctcaac 2160atcgacagct acagcaccta cgacctcagc
ttcagcttca gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg
cgtggtgctc ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca
tctcagagag cttcaccacc gccagcaaca aggatggctt cttcatcgag
2340ctcaccgccg agaggacaag cagcaccttc cacagcttca gagacatcag
catcaaggag 2400aagattgaat aa 2412342412DNAArtificialAxmi115 variant
sequence (Axmi115_Block2_L11G10) 34atgaacatga acaacaccaa gctcaatgca
agggcgctgc cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc
aaggacatca tgaacatgat cttcaagacc 120gacaccggcg gcgacctcac
cttggatgag atcctcaaga accagcagct gctgaatgag 180atctcaggca
agctggacgg cgtcaatgga agcctcaacg acctcattgc tcaaggcaac
240ctcaacaccg agctgagcaa ggagatcctc aagattgcaa atgagcagaa
ccaggtgctg 300aatgatgtca acaacaagct ggacgccatc aacaccatgc
tcaacatcta cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag
cagaactacg cgctgagcct ccagattgag 420tacctctcaa ggcagctgca
agagatctcc gacaagctgg atgtcatcaa cctcaatgtg 480ctcatcaaca
gcaccttgac agagatcacg ccaagctacc agaggatcaa gtatgtcaat
540gagaagttcg acaagctcac cttcgccacc gagagcaccc tccgcgccaa
gcaaggcatc 600ttcaatgaag attcatttga caacaacacc ttggagaact
tgacagacct cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac
agcttcgagt tctacctcca caccttccat 720gatgtgctca tcggcaacaa
cctctttgga agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg
atgagatcaa gacaagcggc agcgagatcg gcaaggtcta cagcttcctc
840atcgtgctga catcattgca agccaaggcc ttcctcacct tgacaacctg
ccgcaagttg 900ctgggcctct ccgacatcga ctacacctcc atcatgaatg
agcacctcaa caatgagaag 960aatgagttca gagacaacat cctgccggcg
ctgagcaaca agttcagcaa cccaagctac 1020gccaagacca tcggctcaga
caactacgcc aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg
gcttcgagat catcaatgat ccaattcctg ttctcaaggc ctacaaggcc
1140aagctgaagc agaactacca ggtggacaac cagagcttga gcgagatcgt
ctacctggac 1200atcgacaagc tcttctgccc ggagaactca gagcagaagt
actacaccaa gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc
accttcgaga agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt
ctatgatcca tcaacaggag acatcgacct caacaagaag 1380caagtggaga
gcaccttccc tcaaacagac tacatcacca tggacattgg agatgatgat
1440ggcatctaca tgccgctcgg cgtcatctca gaaaccttct tgacgcccat
caacagcttc 1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca
agtgcaagag ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac
aaggagacag ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt
gaagaactgg gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca
acaagaatgc ttatgtggac aacaccggcg gcattgaaag aagcaaggcg
1740ctcttcaccc aaagtgatgg agagttcagc cagttcatcg gcgacaagct
aaagcccaac 1800accgactaca tcatccagta caccgtcaag ggcaagccgg
ccatctacct caagaacaag 1860agcaccggct acatcaccta cgaggacacc
aatggaaatt ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga
aacagatttg agccagaccc acctggtgtt caagagccaa 1980aatggatatg
aagcatgggg agacaacttc atcatcctgg aggccaagct cttcgagaca
2040ccagaaagcc cggagctcat caagttcaat gattgggaga ggttcggcac
cacctacatc 2100accggcaatg agctgaggat tgatcattca agaggaggct
acttccgcca aagcctcaac 2160atcgacagct acagcaccta cgacctcagc
ttcagcttca gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg
cgtggtgctc ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca
tctcagagag cttcaccacc gccagcaaca aggatggctt cttcatcgag
2340ctcaccgccg agaggacaag cagcaccttc cacagcttca gagacatcag
catcaaggag 2400aagattgaat aa 2412352412DNAArtificial
sequenceAxmi115 variant sequence (Axmi115_Block2_L12C3)
35atgaacatga acaacaccaa gctcaatgca agggcgctgc cgagcttcat cgactacttc
60aatggcatct atggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc
120gacaccggcg gcgacctcac cttggatgag atcctcaaga accagcagct
gctgaatgag 180atctcaggca agctggacgg cgtcaatgga agcctcaacg
acctcattgc tcaaggcaac 240ctcaacaccg agctgagcaa ggagatcctc
aagattgcaa atgagcagaa ccaggtgctg 300aatgatgtca acaacaagct
ggacgccatc aacaccatgc tcaacatcta cctgccaaag 360atcacctcaa
tgctctctga tgtgatgaag cagaactacg cgctgagcct ccagattgag
420tacctctcaa ggcagctgca agagatctcc gacaagctgg atgtcatcaa
cctcaatgtg 480ctcatcaaca gcaccttgac agagatcacg ccaagctacc
agaggatcaa gtatgtcaat 540gagaagttcg acaagctcac cttcgccacc
gagagcaccc tccgcgccaa gcaaggcatc 600ttcaatgaag attcatttga
caacaacacc ttggagaact tgacagacct cgccgagctg 660gccaagagca
tcaccaagaa tgatgtggac agcttcgagt tctacctcca caccttccat
720gatgtgctca tcggcaacaa cctctttgga agaagcgcgc tcaagacggc
atcagagctc 780atcaccaagg atgagatcaa gacaagcggc agcgagatcg
gcaaggtcta cagcttcctc 840atcgtgctga catcattgca agccaaggcc
ttcctcacct tgacaacctg ccgcaagttg 900ctgggcctct ccgacatcga
ctacacctcc atcatgaatg agcacctcaa caatgagaag 960aatgagttca
gagacaacat cctgccggcg ctgagcaaca agttcagcaa cccaagctac
1020gccaagacca tcggctcaga caactacgcc aaggtgatcc tggagagcga
gcctggctac 1080gcgctggtgg gcttcgagat catcaatgat ccaattcctg
ttctcaaggc ctacaaggcc 1140aagctgaagc agaactacca ggtggacaac
cagagcttga gcgagatcgt ctacctggac 1200atcgacaagc tcttctgccc
ggagaactca gagcagaagt actacaccaa gaacctcacc 1260ttccctgatg
gatatgtcat caccaagatc accttcgaga agaagctgaa caacctcatc
1320tacgaggcca ccgccaactt ctatgatcca tcaacaggag acatcgacct
caacaagaag 1380caagtggaga gcaccttccc tcaaacagac tacatcacca
tggacattgg agatgatgat 1440ggcatctaca tgccgctcgg cgtcatctca
gaaaccttct tgacgcccat caacagcttc 1500ggcctggagg tggacgccaa
gagcaagacc ttgacgctca agtgcaagag ctacctcagg 1560gagtacctgc
tggagagtga tttgaagaac aaggagacag ggctgatcgc gccgccaaat
1620gtgttcatca gcaatgtggt gaagaactgg gacatcgagg aggattcatt
ggagccatgg 1680gtggccaaca acaagaatgc ttatgtggac aacaccggcg
gcattgaaag aagcaaggcg 1740ctcttcaccc aaggagatgg agttttcagc
cagttcatcg gcgacaagct aaagcccaac 1800accgactaca tcatccagta
caccgtcaag ggcaagccgg ccatctacct caagaacaag 1860agcaccggct
acatcaccta cgaggacacc aatggaaatt ctgaggagtt ccaaacaatt
1920gctgtgaagt tcacctcaga aacagatttg agccagaccc acctggtgtt
caagagccaa 1980aatggatatg aagcatgggg agacaacttc atcatcctgg
aggccaagct cttcgagaca 2040ccagaaagcc cggagctcat caagttcaat
gattgggaga ggttcggcac cacctacatc 2100accggcaatg agctgaggat
tgatcattca agaggaggct acttccgcca aagcctcaac 2160atcgacagct
acagcaccta cgacctcagc ttcagcttca gcggcctctg ggccaaggtg
2220attgtgaaga acagccgcgg cgtggtgctc ttcgagaagg tgaagaacaa
tggaagcagc 2280tatgaggaca tctcagagag cttcaccacc gccagcaaca
aggatggctt cttcatcgag 2340ctcaccgccg agaggacaag cagcaccttc
cacagcttca gagacatcag catcaaggag 2400aagattgaat aa
2412362412DNAArtificial sequenceAxmi115 variant sequence
(Axmi115_Block18_ L12A10) 36atgaacatga acaacaccaa gctcaatgca
agggcgctgc cgagcttcat cgactacttc 60aatggcatct atggcttcgc caccggcatc
aaggacatca tgaacatgat cttcaagacc 120gacaccggcg gcgacctcac
cttggatgag atcctcaaga accagcagct gctgaatgag 180atctcaggca
agctggacgg cgtcaatgga agcctcaacg acctcattgc tcaaggcaac
240ctcaacaccg agctgagcaa ggagatcctc aagattgcaa atgagcagaa
ccaggtgctg 300aatgatgtca acaacaagct ggacgccatc aacaccatgc
tcaacatcta cctgccaaag 360atcacctcaa tgctctctga tgtgatgaag
cagaactacg cgctgagcct ccagattgag 420tacctctcaa ggcagctgca
agagatctcc gacaagctgg atgtcatcaa cctcaatgtg 480ctcatcaaca
gcaccttgac agagatcacg ccaagctacc agaggatcaa gtatgtcaat
540gagaagttcg acaagctcac cttcgccacc gagagcaccc tccgcgccaa
gcaaggcatc 600ttcaatgaag attcatttga caacaacacc ttggagaact
tgacagacct cgccgagctg 660gccaagagca tcaccaagaa tgatgtggac
agcttcgagt tctacctcca caccttccat 720gatgtgctca tcggcaacaa
cctctttgga agaagcgcgc tcaagacggc atcagagctc 780atcaccaagg
atgagatcaa gacaagcggc agcgagatcg gcaaggtcta cagcttcctc
840atcgtgctga catcattgca agccaaggcc ttcctcacct tgacaacctg
ccgcaagttg 900ctgggcctct ccgacatcga ctacacctcc atcatgaatg
agcacctcaa caatgagaag 960aatgagttca gagacaacat cctgccggcg
ctgagcaaca agttcagcaa cccaagctac 1020gccaagacca tcggctcaga
caactacgcc aaggtgatcc tggagagcga gcctggctac 1080gcgctggtgg
gcttcgagat catcaatgat ccaattcctg ttctcaaggc ctacaaggcc
1140aagctgaagc agaactacca ggtggacaac cagagcttga gcgagatcgt
ctacctggac 1200atcgacaagc tcttctgccc ggagaactca gagcagaagt
actacaccaa gaacctcacc 1260ttccctgatg gatatgtcat caccaagatc
accttcgaga agaagctgaa caacctcatc 1320tacgaggcca ccgccaactt
ctatgatcca tcaacaggag acatcgacct caacaagaag 1380caagtggaga
gcaccttccc tcaaacagac tacatcacca tggacattgg agatgatgat
1440ggcatctaca tgccgctcgg cgtcatctca gaaaccttct tgacgcccat
caacagcttc 1500ggcctggagg tggacgccaa gagcaagacc ttgacgctca
agtgcaagag ctacctcagg 1560gagtacctgc tggagagtga tttgaagaac
aaggagacag ggctgatcgc gccgccaaat 1620gtgttcatca gcaatgtggt
gaagaactgg gacatcgagg aggattcatt ggagccatgg 1680gtggccaaca
acaagaatgc ttatgtggac aacaccggcg gcattgaaag aagcaaggcg
1740ctcttcaccc aaggagatgg agagttcagc cagttcatcg gcgacaagct
aaagcccaac 1800accgactaca tcatccagta caccgtcaag ggcaagccgg
ccatctacct caagaacaag 1860agcaccggct acatcaccta cgaggacacc
aatggaaatt ctgaggagtt ccaaacaatt 1920gctgtgaagt tcacctcaga
aacagatttg agccagaccc acctggtgtt caagagccaa 1980aatggatatg
aagcatgggg agacaacttc atcatcctgg aggccaagct cttcgagaca
2040ccagaaagcc cggagctcat caagttcaat gattgggaga ggttcggcac
cacctacatc 2100accggcaatg agctgaggat tgatcattca agaggaggct
acttccgcca aagcctcaac 2160atcgacagct acagcaccta cgacctcagc
ttcagcttca gcggcctctg ggccaaggtg 2220attgtgaaga acagccgcgg
cgtggtgctc ttcgagaagg tgaagaacaa tggaagcagc 2280tatgaggaca
tctcagagag cttcaccacc tgcagcaaca aggatggctt cttcatcgag
2340ctcaccgccg agaggacaag cagcaccttc cacagcttca gagacatcag
catcaaggag 2400aagattgaat aa 241237803PRTArtificial sequenceAXMI115
variant sequence (AXMI115v02) 37Met Asn Met Asn Asn Thr Lys Leu Asn
Ala Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile
Tyr Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile
Phe Lys Thr Asp Thr Gly Gly Asn Leu Thr Leu 35 40 45 Asp Glu Ile
Leu Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu
Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70
75 80 Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu
Gln 85 90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala
Ile Asn Thr 100 105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser
Met Leu Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu
Gln Ile Glu Tyr Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp
Lys Leu Asp Ile Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser
Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr
Val Asn Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190
Thr Leu Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195
200 205 Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser
Ile 210 215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His
Thr Phe His 225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly
Arg Ser Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp
Glu Ile Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser
Phe Leu Ile Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu
Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile
Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315
320 Asn Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser
325 330 335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala
Lys Val 340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly
Phe Glu Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr
Lys Ala Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser
Leu Ser Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe
Cys Pro Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu
Thr Phe Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu
Lys Lys Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440
445 Asp Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser
450 455 460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp
Asp Asp 465 470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu
Thr Phe Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp
Ala Lys Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu
Arg Glu Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr
Gly Leu Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val
Lys
Asn Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val
Ala Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570
575 Arg Ser Lys Ala Leu Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln Phe
580 585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln
Tyr Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys
Ser Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser
Glu Glu Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu
Thr Asp Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn
Gly Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala
Lys Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe
Asn Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695
700 Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn
705 710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe
Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly
Val Val Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr
Glu Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr Ala Ser Asn Lys Asp
Gly Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr
Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile
Glu 38798PRTArtificial sequenceAXMI115 variant sequence (AXMI115,
expressed from pAX6308) 38Met Asn Met Asn Asn Thr Lys Leu Asn Ala
Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr
Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe
Lys Thr Asp Thr Gly Gly Asn Leu Thr Leu 35 40 45 Asp Glu Ile Leu
Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp
Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85
90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn
Thr 100 105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu
Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile
Glu Tyr Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu
Asp Ile Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu
Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn
Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr 180 185 190 Thr Leu
Lys Val Lys Lys Asp Ser Ser Pro Ala Asp Ile Leu Asp Glu 195 200 205
Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val 210
215 220 Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val
Gly 225 230 235 240 Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala
Ser Glu Leu Ile 245 250 255 Ala Lys Glu Asn Val Lys Thr Ser Gly Ser
Glu Val Gly Asn Val Tyr 260 265 270 Asn Phe Leu Ile Val Leu Thr Ala
Leu Gln Ala Lys Ala Phe Leu Thr 275 280 285 Leu Thr Thr Cys Arg Lys
Leu Leu Gly Leu Ser Asp Ile Asp Tyr Thr 290 295 300 Ser Ile Met Asn
Glu His Leu Asn Asn Glu Lys Asn Glu Phe Arg Asp 305 310 315 320 Asn
Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser Asn Pro Ser Tyr Ala 325 330
335 Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val Ile Leu Glu Ser Glu
340 345 350 Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile Ile Asn Asp Pro
Ile Pro 355 360 365 Val Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln Asn
Tyr Gln Val Asp 370 375 380 Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu
Asp Ile Asp Lys Leu Phe 385 390 395 400 Cys Pro Glu Asn Ser Glu Gln
Lys Tyr Tyr Thr Lys Asn Leu Thr Phe 405 410 415 Pro Asp Gly Tyr Val
Ile Thr Lys Ile Thr Phe Glu Lys Lys Leu Asn 420 425 430 Asn Leu Ile
Tyr Glu Ala Thr Ala Asn Phe Tyr Asp Pro Ser Thr Gly 435 440 445 Asp
Ile Asp Leu Asn Lys Lys Gln Val Glu Ser Thr Phe Pro Gln Thr 450 455
460 Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp Gly Ile Tyr Met Pro
465 470 475 480 Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn
Ser Phe Gly 485 490 495 Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr
Leu Lys Cys Lys Ser 500 505 510 Tyr Leu Arg Glu Tyr Leu Leu Glu Ser
Asp Leu Lys Asn Lys Glu Thr 515 520 525 Gly Leu Ile Ala Pro Pro Asn
Val Phe Ile Ser Asn Val Val Lys Asn 530 535 540 Trp Asp Ile Glu Glu
Asp Ser Leu Glu Pro Trp Val Ala Asn Asn Lys 545 550 555 560 Asn Ala
Tyr Val Asp Asn Thr Gly Gly Ile Glu Arg Ser Lys Ala Leu 565 570 575
Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln Phe Ile Gly Asp Lys Leu 580
585 590 Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr Val Lys Gly Lys
Pro 595 600 605 Ala Ile Tyr Leu Lys Asn Lys Ser Thr Gly Tyr Ile Thr
Tyr Glu Asp 610 615 620 Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile
Ala Val Lys Phe Thr 625 630 635 640 Ser Glu Thr Asp Leu Ser Gln Thr
His Leu Val Phe Lys Ser Gln Asn 645 650 655 Gly Tyr Glu Ala Trp Gly
Asp Asn Phe Ile Ile Leu Glu Ala Lys Leu 660 665 670 Phe Glu Thr Pro
Glu Ser Pro Glu Leu Ile Lys Phe Asn Asp Trp Glu 675 680 685 Arg Phe
Gly Thr Thr Tyr Ile Thr Gly Asn Glu Leu Arg Ile Asp His 690 695 700
Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn Ile Asp Ser Tyr Ser 705
710 715 720 Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly Leu Trp Ala Lys
Val Ile 725 730 735 Val Lys Asn Ser Arg Gly Val Val Leu Phe Glu Lys
Val Lys Asn Asn 740 745 750 Gly Ser Ser Tyr Glu Asp Ile Ser Glu Ser
Phe Thr Thr Ala Ser Asn 755 760 765 Lys Asp Gly Phe Phe Ile Glu Leu
Thr Ala Glu Arg Thr Ser Ser Thr 770 775 780 Phe His Ser Phe Arg Asp
Ile Ser Ile Lys Glu Lys Ile Glu 785 790 795 39795PRTArtificial
sequenceAXMI115 variant sequence (AXMI115, expressed from pAX6309)
39Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1
5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys
Asp 20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asn
Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn
Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn
Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys
Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn
Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu His
Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met
Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys 130 135
140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val
145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr
Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr
Phe Ala Thr Glu Thr 180 185 190 Thr Leu Lys Val Lys Lys Asp Ser Ser
Pro Ala Asp Ile Leu Asp Glu 195 200 205 Leu Thr Glu Leu Thr Glu Leu
Ala Lys Ser Val Thr Lys Asn Asp Val 210 215 220 Asp Gly Phe Glu Phe
Tyr Leu Asn Thr Phe His Asp Val Met Val Gly 225 230 235 240 Asn Asn
Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile 245 250 255
Ala Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr 260
265 270 Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Lys Ala Phe Leu
Thr 275 280 285 Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile
Asp Tyr Thr 290 295 300 Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys
Glu Glu Phe Arg Val 305 310 315 320 Asn Ile Leu Pro Thr Leu Ser Asn
Thr Phe Ser Asn Pro Asn Tyr Ala 325 330 335 Lys Val Lys Gly Ser Asp
Glu Asp Ala Lys Met Ile Val Glu Ala Lys 340 345 350 Pro Gly His Ala
Leu Val Gly Phe Glu Met Ser Asn Asp Ser Ile Thr 355 360 365 Val Leu
Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp 370 375 380
Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu 385
390 395 400 Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile
Val Phe 405 410 415 Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr
Lys Lys Met Lys 420 425 430 Thr Leu Arg Tyr Glu Val Thr Ala Asn Ser
Tyr Asp Ser Ser Thr Gly 435 440 445 Glu Ile Asp Leu Asn Lys Lys Lys
Val Glu Ser Ser Glu Ala Glu Tyr 450 455 460 Arg Thr Leu Ser Ala Lys
Asp Asp Gly Val Tyr Met Pro Leu Gly Val 465 470 475 480 Ile Ser Glu
Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala 485 490 495 Asp
Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg 500 505
510 Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile
515 520 525 Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly
Asn Leu 530 535 540 Glu Gly Glu Asn Leu Glu Pro Trp Ile Ala Asn Asn
Lys Asn Ala Tyr 545 550 555 560 Val Asp His Thr Gly Gly Val Asn Gly
Thr Arg Ala Leu Tyr Val His 565 570 575 Lys Asp Gly Gly Phe Ser Gln
Phe Ile Gly Asp Lys Leu Lys Pro Lys 580 585 590 Thr Glu Tyr Val Ile
Gln Tyr Thr Val Lys Gly Lys Pro Ala Ile Tyr 595 600 605 Leu Lys Asn
Lys Ser Thr Gly Tyr Ile Thr Tyr Glu Asp Thr Asn Gly 610 615 620 Asn
Ser Glu Glu Phe Gln Thr Ile Ala Val Lys Phe Thr Ser Glu Thr 625 630
635 640 Asp Leu Ser Gln Thr His Leu Val Phe Lys Ser Gln Asn Gly Tyr
Glu 645 650 655 Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ala Lys Leu
Phe Glu Thr 660 665 670 Pro Glu Ser Pro Glu Leu Ile Lys Phe Asn Asp
Trp Glu Arg Phe Gly 675 680 685 Thr Thr Tyr Ile Thr Gly Asn Glu Leu
Arg Ile Asp His Ser Arg Gly 690 695 700 Gly Tyr Phe Arg Gln Ser Leu
Asn Ile Asp Ser Tyr Ser Thr Tyr Asp 705 710 715 720 Leu Ser Phe Ser
Phe Ser Gly Leu Trp Ala Lys Val Ile Val Lys Asn 725 730 735 Ser Arg
Gly Val Val Leu Phe Glu Lys Val Lys Asn Asn Gly Ser Ser 740 745 750
Tyr Glu Asp Ile Ser Glu Ser Phe Thr Thr Ala Ser Asn Lys Asp Gly 755
760 765 Phe Phe Ile Glu Leu Thr Ala Glu Arg Thr Ser Ser Thr Phe His
Ser 770 775 780 Phe Arg Asp Ile Ser Ile Lys Glu Lys Ile Glu 785 790
795 40803PRTArtificial sequenceAXMI115 variant sequence
(AXMI115v02(EVO27)) 40Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg
Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly
Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe Lys
Thr Asp Thr Gly Gly Asn Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys
Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly
Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu
Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90
95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr
100 105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser
Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu
Tyr Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp
Ile Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu Thr
Glu Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu
Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg
Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn
Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215
220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His
225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala
Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys
Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile
Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr
Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr
Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu
Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335
Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340
345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile
Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys
Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu
Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu
Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro
Asp Gly Tyr Val Ile Thr Lys Ile Thr
Phe 420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala
Asn Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys
Lys Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr
Met Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr Met Pro Leu
Gly Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile Asn Ser Phe
Gly Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505 510 Leu Lys
Cys Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu 515 520 525
Lys Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe Ile Ser 530
535 540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro
Trp 545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr
Gly Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Thr Gln Gly Asp
Gly Lys Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu Lys Pro Asn
Thr Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly Lys Pro Ala
Ile Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu
Asp Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630 635 640 Ala
Val Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu Val 645 650
655 Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile
660 665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu
Ile Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile
Thr Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg Gly Gly Tyr
Phe Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr Ser Thr Tyr
Asp Leu Ser Phe Ser Phe Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile
Val Lys Asn Ser Arg Gly Val Val Leu Phe Glu 740 745 750 Lys Val Lys
Asn Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu Ser Phe 755 760 765 Thr
Thr Ala Ser Asn Lys Asp Gly Phe Phe Ile Glu Leu Thr Ala Glu 770 775
780 Arg Thr Ser Ser Thr Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu
785 790 795 800 Lys Ile Glu 41803PRTArtificialAXMI115 variant
sequence (AXMI115v02(EVO28)) 41Met Asn Met Asn Asn Thr Lys Leu Asn
Ala Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile
Tyr Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile
Phe Lys Thr Asp Thr Gly Gly Asn Leu Thr Leu 35 40 45 Asp Glu Ile
Leu Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu
Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70
75 80 Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu
Gln 85 90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala
Ile Asn Thr 100 105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser
Met Leu Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu
Gln Ile Glu Tyr Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp
Lys Leu Asp Ile Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser
Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr
Val Asn Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190
Thr Leu Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195
200 205 Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser
Ile 210 215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His
Thr Phe His 225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly
Arg Ser Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp
Glu Ile Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser
Phe Leu Ile Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu
Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile
Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315
320 Asn Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser
325 330 335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala
Lys Val 340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly
Phe Glu Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr
Lys Ala Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser
Leu Ser Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe
Cys Pro Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu
Thr Phe Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu
Lys Lys Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440
445 Asp Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser
450 455 460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp
Asp Asp 465 470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu
Thr Phe Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp
Ala Lys Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu
Arg Glu Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr
Gly Leu Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val
Lys Asn Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560
Val Ala Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565
570 575 Arg Ser Lys Ala Leu Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln
Phe 580 585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile
Gln Tyr Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn
Lys Ser Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn
Ser Glu Glu Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser
Glu Thr Asp Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln
Asn Gly Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu
Ala Lys Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685
Phe Asn Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690
695 700 Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu
Asn 705 710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser
Phe Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg
Gly Val Val Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser
Tyr Glu Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr Met Ser Asn Lys
Asp Gly Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser
Thr Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys
Ile Glu 42803PRTArtificialAXMI115 variant sequence
(AXMI115v02(EVO29)) 42Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg
Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly
Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe Lys
Thr Asp Thr Gly Gly Asn Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys
Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly
Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu
Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90
95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr
100 105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser
Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu
Tyr Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp
Ile Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu Thr
Glu Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu
Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg
Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn
Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215
220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His
225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala
Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys
Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile
Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr
Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr
Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu
Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335
Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340
345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile
Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys
Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu
Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu
Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro
Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu
Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro
Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455 460
Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465
470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu
Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser
Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr
Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile
Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn Trp
Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala Asn
Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg
Ser Lys Ala Leu Phe Thr Val Gly Asp Gly Glu Phe Ser Gln Phe 580 585
590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr
595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr
Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu
Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp
Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr
Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu
Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp
Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu
Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710
715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly
Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val Val
Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu Asp
Ile Ser Glu Ser Phe 755 760 765 Thr Thr Ala Ser Asn Lys Asp Gly Phe
Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe His
Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
43803PRTArtificialAXMI115 variant sequence (AXMI115v02(EVO31))
43Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1
5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys
Asp 20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asn
Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn
Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn
Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys
Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn
Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu His
Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met
Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys 130 135
140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val
145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr
Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr
Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe
Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr
Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215 220 Thr Lys Asn Asp Val
Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His 225 230 235 240 Asp Val
Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr 245 250 255
Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly Ser Glu 260
265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile Val Leu Thr Ser Leu Gln
Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu
Gly Leu Ser 290
295 300 Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu
Lys 305 310 315 320 Asn Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser
Asn Lys Phe Ser 325 330 335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser
Asp Asn Tyr Ala Lys Val 340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr
Ala Leu Val Gly Phe Glu Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val
Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val
Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile
Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410
415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe
420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn
Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys
Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met
Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr Met Pro Leu Gly
Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly
Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys
Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys
Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535
540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp
545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly
Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Thr Gln Gly Asp Gly
Glu Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr
Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile
Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp
Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630 635 640 Ala Val
Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu Val 645 650 655
Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660
665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile
Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr
Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe
Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp
Leu Ser Phe Ser Phe Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile Val
Lys Asn Ser Arg Gly Val Val Leu Phe Glu 740 745 750 Lys Val Lys Asn
Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu Asp Phe 755 760 765 Thr Thr
Asn Gly Phe Lys Asp Gly Phe Tyr Ile Glu Leu Thr Ala Glu 770 775 780
Arg Thr Ser Ser Thr Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785
790 795 800 Lys Ile Glu 44803PRTArtificialAXMI115 variant sequence
(AXMI115v02(EVO32)) 44Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg
Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly
Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe Lys
Thr Asp Thr Gly Gly Asn Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys
Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly
Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu
Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90
95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr
100 105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser
Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu
Tyr Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp
Ile Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu Thr
Glu Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu
Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg
Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn
Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215
220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His
225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala
Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys
Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile
Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr
Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr
Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu
Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335
Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340
345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile
Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys
Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu
Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu
Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro
Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu
Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro
Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455 460
Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465
470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu
Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser
Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr
Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile
Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn Trp
Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala Asn
Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg
Ser Lys Ala Leu Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln Phe 580 585
590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr
595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr
Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu
Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp
Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr
Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu
Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp
Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu
Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710
715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly
Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val Val
Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu Asp
Ile Ser Glu His Phe 755 760 765 Thr Thr Trp Gly Tyr Lys Asp Gly Phe
Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe His
Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
45803PRTArtificialAXMI115 variant sequence (AXMI115_Block2_L11C7)
45Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1
5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys
Asp 20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp
Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn
Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn
Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys
Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn
Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu Asn
Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met
Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Arg 130 135
140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Val Ile Asn Leu Asn Val
145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ser Tyr
Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr
Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe
Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr
Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215 220 Thr Lys Asn Asp Val
Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His 225 230 235 240 Asp Val
Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr 245 250 255
Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly Ser Glu 260
265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile Val Leu Thr Ser Leu Gln
Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu
Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His
Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu Phe Arg Asp Asn Ile Leu
Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335 Asn Pro Ser Tyr Ala Lys
Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340 345 350 Ile Leu Glu Ser
Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile Ile 355 360 365 Asn Asp
Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln 370 375 380
Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu Asp 385
390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu Gln Lys Tyr
Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr Val Ile Thr
Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu
Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp
Leu Asn Lys Lys Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp
Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr
Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile
Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505
510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu
515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe
Ile Ser 530 535 540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser
Leu Glu Pro Trp 545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val
Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Val
Gln Gly Asp Gly Glu Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu
Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly
Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile
Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630
635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu
Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn
Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser
Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr
Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg
Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr
Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly Leu 725 730 735 Trp Ala
Lys Val Ile Val Lys Asn Ser Arg Gly Val Val Leu Phe Glu 740 745 750
Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu Ser Phe 755
760 765 Thr Thr Ala Ser Asn Lys Asp Gly Phe Phe Ile Glu Leu Thr Ala
Glu 770 775 780 Arg Thr Ser Ser Thr Phe His Ser Phe Arg Asp Ile Ser
Ile Lys Glu 785 790 795 800 Lys Ile Glu 46803PRTArtificialAXMI115
variant sequence (AXMI115_Block2_L11H6) 46Met Asn Met Asn Asn Thr
Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe
Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met
Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu 35 40 45
Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50
55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly
Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala
Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu
Asp Ala Ile Asn Thr 100 105 110 Met Leu Asn Ile Tyr Leu Pro Lys Ile
Thr Ser Met Leu Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu
Ser Leu Gln Ile Glu Tyr Leu Ser Arg 130 135 140 Gln Leu Gln Glu Ile
Ser Asp Lys Leu Asp Val Ile Asn Leu Asn Val 145 150 155 160 Leu Ile
Asn Ser Thr Leu Thr Glu Ile Thr Pro Ser Tyr Gln Arg Ile 165
170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu
Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser
Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu
Leu Ala Lys Ser Ile 210 215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu
Phe Tyr Leu His Thr Phe His 225 230 235 240 Asp Val Leu Ile Gly Asn
Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu
Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly
Lys Val Tyr Ser Phe Leu Ile Val Leu Thr Ser Leu Gln Ala 275 280 285
Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290
295 300 Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu
Lys 305 310 315 320 Asn Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser
Asn Lys Phe Ser 325 330 335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser
Asp Asn Tyr Ala Lys Val 340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr
Ala Leu Val Gly Phe Glu Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val
Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val
Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile
Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410
415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe
420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn
Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys
Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met
Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr Met Pro Leu Gly
Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly
Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys
Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys
Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535
540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp
545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly
Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Thr Gln Gly Asp Gly
Lys Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr
Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile
Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp
Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630 635 640 Ala Val
Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu Val 645 650 655
Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660
665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile
Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr
Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe
Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp
Leu Ser Phe Ser Phe Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile Val
Lys Asn Ser Arg Gly Val Val Leu Phe Glu 740 745 750 Lys Val Lys Asn
Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr
Ala Ser Asn Lys Asp Gly Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780
Arg Thr Ser Ser Thr Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785
790 795 800 Lys Ile Glu 47803PRTArtificialAXMI115 variant sequence
(AXMI115_Block2_ L11H7) 47Met Asn Met Asn Asn Thr Lys Leu Asn Ala
Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr
Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe
Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu 35 40 45 Asp Glu Ile Leu
Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp
Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85
90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn
Thr 100 105 110 Met Leu Asn Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu
Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile
Glu Tyr Leu Ser Arg 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu
Asp Val Ile Asn Leu Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu
Thr Glu Ile Thr Pro Ser Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn
Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu
Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205
Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210
215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe
His 225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser
Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile
Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu
Ile Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu
Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr
Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn
Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330
335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val
340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu
Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala
Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser
Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro
Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe
Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys
Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp
Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455
460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp
465 470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe
Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys
Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu
Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu
Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn
Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala
Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575
Arg Ser Lys Ala Leu Phe Thr Leu Gly Asp Gly Glu Phe Ser Gln Phe 580
585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr
Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser
Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu
Glu Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr
Asp Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly
Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys
Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn
Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700
Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705
710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser
Gly Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val
Val Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu
Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr Ala Ser Asn Lys Asp Gly
Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe
His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
48803PRTArtificialAxmi115 variant sequence (AXMI115_Block2_ L11A9)
48Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1
5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys
Asp 20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp
Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn
Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn
Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys
Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn
Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu Asn
Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met
Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Arg 130 135
140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Val Ile Asn Leu Asn Val
145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ser Tyr
Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr
Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe
Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr
Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215 220 Thr Lys Asn Asp Val
Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His 225 230 235 240 Asp Val
Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr 245 250 255
Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly Ser Glu 260
265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile Val Leu Thr Ser Leu Gln
Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu
Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His
Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu Phe Arg Asp Asn Ile Leu
Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335 Asn Pro Ser Tyr Ala Lys
Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340 345 350 Ile Leu Glu Ser
Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile Ile 355 360 365 Asn Asp
Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln 370 375 380
Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu Asp 385
390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu Gln Lys Tyr
Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr Val Ile Thr
Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu
Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp
Leu Asn Lys Lys Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp
Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr
Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile
Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505
510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu
515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe
Ile Ser 530 535 540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser
Leu Glu Pro Trp 545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val
Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Thr
Lys Gly Asp Gly Glu Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu
Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly
Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile
Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630
635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu
Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn
Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser
Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr
Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg
Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr
Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly Leu 725 730 735 Trp Ala
Lys Val Ile Val Lys Asn Ser Arg Gly Val Val Leu Phe Glu 740 745 750
Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu Ser Phe 755
760 765 Thr Thr Ala Ser Asn Lys Asp Gly Phe Phe Ile Glu Leu Thr Ala
Glu 770 775 780 Arg Thr Ser Ser Thr Phe His Ser Phe Arg Asp Ile Ser
Ile Lys Glu 785 790 795 800 Lys Ile Glu 49803PRTArtificialAXMI115
variant sequence (AXMI115_Block2_L11F9) 49Met Asn Met Asn Asn Thr
Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe
Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met
Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu 35
40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly
Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala
Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys
Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn Asp Val Asn Asn
Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu Asn Ile Tyr Leu Pro
Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr
Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Arg 130 135 140 Gln Leu Gln
Glu Ile Ser Asp Lys Leu Asp Val Ile Asn Leu Asn Val 145 150 155 160
Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ser Tyr Gln Arg Ile 165
170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu
Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser
Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu
Leu Ala Lys Ser Ile 210 215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu
Phe Tyr Leu His Thr Phe His 225 230 235 240 Asp Val Leu Ile Gly Asn
Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu
Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly
Lys Val Tyr Ser Phe Leu Ile Val Leu Thr Ser Leu Gln Ala 275 280 285
Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290
295 300 Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu
Lys 305 310 315 320 Asn Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser
Asn Lys Phe Ser 325 330 335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser
Asp Asn Tyr Ala Lys Val 340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr
Ala Leu Val Gly Phe Glu Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val
Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val
Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile
Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410
415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe
420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn
Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys
Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met
Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr Met Pro Leu Gly
Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly
Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys
Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys
Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535
540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp
545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly
Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Thr Val Gly Asp Gly
Lys Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr
Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile
Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp
Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630 635 640 Ala Val
Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu Val 645 650 655
Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660
665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile
Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr
Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe
Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp
Leu Ser Phe Ser Phe Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile Val
Lys Asn Ser Arg Gly Val Val Leu Phe Glu 740 745 750 Lys Val Lys Asn
Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr
Ala Ser Asn Lys Asp Gly Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780
Arg Thr Ser Ser Thr Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785
790 795 800 Lys Ile Glu 50803PRTArtificialAXMI115 variant sequence
(AXMI115_Block2_L11G10) 50Met Asn Met Asn Asn Thr Lys Leu Asn Ala
Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr
Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe
Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu 35 40 45 Asp Glu Ile Leu
Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp
Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85
90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn
Thr 100 105 110 Met Leu Asn Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu
Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile
Glu Tyr Leu Ser Arg 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu
Asp Val Ile Asn Leu Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu
Thr Glu Ile Thr Pro Ser Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn
Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu
Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205
Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210
215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe
His 225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser
Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile
Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu
Ile Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu
Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr
Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn
Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330
335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val
340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu
Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala
Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser
Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro
Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe
Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys
Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp
Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455
460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp
465 470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe
Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys
Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu
Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu
Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn
Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala
Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575
Arg Ser Lys Ala Leu Phe Thr Gln Ser Asp Gly Glu Phe Ser Gln Phe 580
585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr
Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser
Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu
Glu Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr
Asp Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly
Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys
Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn
Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700
Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705
710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser
Gly Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val
Val Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu
Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr Ala Ser Asn Lys Asp Gly
Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe
His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
51803PRTArtificialAXMI115 variant sequence (AXMI115_Block2_ L12C3)
51Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1
5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys
Asp 20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp
Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn
Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn
Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys
Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn
Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu Asn
Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met
Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Arg 130 135
140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Val Ile Asn Leu Asn Val
145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ser Tyr
Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Asp Lys Leu Thr
Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg Ala Lys Gln Gly Ile Phe
Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn Thr Leu Glu Asn Leu Thr
Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215 220 Thr Lys Asn Asp Val
Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His 225 230 235 240 Asp Val
Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr 245 250 255
Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly Ser Glu 260
265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile Val Leu Thr Ser Leu Gln
Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu
Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His
Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu Phe Arg Asp Asn Ile Leu
Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335 Asn Pro Ser Tyr Ala Lys
Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340 345 350 Ile Leu Glu Ser
Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile Ile 355 360 365 Asn Asp
Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln 370 375 380
Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr Leu Asp 385
390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu Gln Lys Tyr
Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr Val Ile Thr
Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu Asn Asn Leu Ile Tyr Glu
Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro Ser Thr Gly Asp Ile Asp
Leu Asn Lys Lys Gln Val Glu Ser 450 455 460 Thr Phe Pro Gln Thr Asp
Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465 470 475 480 Gly Ile Tyr
Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro 485 490 495 Ile
Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser Lys Thr Leu Thr 500 505
510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu Ser Asp Leu
515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro Asn Val Phe
Ile Ser 530 535 540 Asn Val Val Lys Asn Trp Asp Ile Glu Glu Asp Ser
Leu Glu Pro Trp 545 550 555 560 Val Ala Asn Asn Lys Asn Ala Tyr Val
Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg Ser Lys Ala Leu Phe Thr
Gln Gly Asp Gly Val Phe Ser Gln Phe 580 585 590 Ile Gly Asp Lys Leu
Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr 595 600 605 Val Lys Gly
Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610 615 620 Ile
Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr Ile 625 630
635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln Thr His Leu
Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp Gly Asp Asn
Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu Phe Glu Thr Pro Glu Ser
Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp Trp Glu Arg Phe Gly Thr
Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu Arg Ile Asp His Ser Arg
Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710 715 720 Ile Asp Ser Tyr
Ser Thr Tyr Asp Leu Ser Phe Ser
Phe Ser Gly Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg
Gly Val Val Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser
Tyr Glu Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr Ala Ser Asn Lys
Asp Gly Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser
Thr Phe His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys
Ile Glu 52803PRTArtificialAXMI115 variant sequence
(AXMI115_Block18_ L12A10) 52Met Asn Met Asn Asn Thr Lys Leu Asn Ala
Arg Ala Leu Pro Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr
Gly Phe Ala Thr Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe
Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu 35 40 45 Asp Glu Ile Leu
Lys Asn Gln Gln Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp
Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85
90 95 Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn
Thr 100 105 110 Met Leu Asn Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu
Ser Asp Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile
Glu Tyr Leu Ser Arg 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu
Asp Val Ile Asn Leu Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu
Thr Glu Ile Thr Pro Ser Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn
Glu Lys Phe Asp Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu
Arg Ala Lys Gln Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205
Asn Thr Leu Glu Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210
215 220 Thr Lys Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe
His 225 230 235 240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser
Ala Leu Lys Thr 245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile
Lys Thr Ser Gly Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu
Ile Val Leu Thr Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu
Thr Thr Cys Arg Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr
Thr Ser Ile Met Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn
Glu Phe Arg Asp Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330
335 Asn Pro Ser Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val
340 345 350 Ile Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu
Ile Ile 355 360 365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala
Lys Leu Lys Gln 370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser
Glu Ile Val Tyr Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro
Glu Asn Ser Glu Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe
Pro Asp Gly Tyr Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys
Leu Asn Asn Leu Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp
Pro Ser Thr Gly Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455
460 Thr Phe Pro Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp
465 470 475 480 Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe
Leu Thr Pro 485 490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys
Ser Lys Thr Leu Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu
Tyr Leu Leu Glu Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu
Ile Ala Pro Pro Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn
Trp Asp Ile Glu Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala
Asn Asn Lys Asn Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575
Arg Ser Lys Ala Leu Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln Phe 580
585 590 Ile Gly Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr
Thr 595 600 605 Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser
Thr Gly Tyr 610 615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu
Glu Phe Gln Thr Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr
Asp Leu Ser Gln Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly
Tyr Glu Ala Trp Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys
Leu Phe Glu Thr Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn
Asp Trp Glu Arg Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700
Leu Arg Ile Asp His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705
710 715 720 Ile Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser
Gly Leu 725 730 735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val
Val Leu Phe Glu 740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu
Asp Ile Ser Glu Ser Phe 755 760 765 Thr Thr Cys Ser Asn Lys Asp Gly
Phe Phe Ile Glu Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe
His Ser Phe Arg Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
53803PRTArtificialAXMI115 variant sequence (AXMI115_Block18_
L12B10) 53Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro
Ser Phe 1 5 10 15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr
Gly Ile Lys Asp 20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr
Gly Gly Asp Leu Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln
Leu Leu Asn Glu Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly
Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu
Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln
Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110
Met Leu Asn Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115
120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser
Arg 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Val Ile Asn
Leu Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr
Pro Ser Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Asp
Lys Leu Thr Phe Ala Thr Glu Ser 180 185 190 Thr Leu Arg Ala Lys Gln
Gly Ile Phe Asn Glu Asp Ser Phe Asp Asn 195 200 205 Asn Thr Leu Glu
Asn Leu Thr Asp Leu Ala Glu Leu Ala Lys Ser Ile 210 215 220 Thr Lys
Asn Asp Val Asp Ser Phe Glu Phe Tyr Leu His Thr Phe His 225 230 235
240 Asp Val Leu Ile Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr
245 250 255 Ala Ser Glu Leu Ile Thr Lys Asp Glu Ile Lys Thr Ser Gly
Ser Glu 260 265 270 Ile Gly Lys Val Tyr Ser Phe Leu Ile Val Leu Thr
Ser Leu Gln Ala 275 280 285 Lys Ala Phe Leu Thr Leu Thr Thr Cys Arg
Lys Leu Leu Gly Leu Ser 290 295 300 Asp Ile Asp Tyr Thr Ser Ile Met
Asn Glu His Leu Asn Asn Glu Lys 305 310 315 320 Asn Glu Phe Arg Asp
Asn Ile Leu Pro Ala Leu Ser Asn Lys Phe Ser 325 330 335 Asn Pro Ser
Tyr Ala Lys Thr Ile Gly Ser Asp Asn Tyr Ala Lys Val 340 345 350 Ile
Leu Glu Ser Glu Pro Gly Tyr Ala Leu Val Gly Phe Glu Ile Ile 355 360
365 Asn Asp Pro Ile Pro Val Leu Lys Ala Tyr Lys Ala Lys Leu Lys Gln
370 375 380 Asn Tyr Gln Val Asp Asn Gln Ser Leu Ser Glu Ile Val Tyr
Leu Asp 385 390 395 400 Ile Asp Lys Leu Phe Cys Pro Glu Asn Ser Glu
Gln Lys Tyr Tyr Thr 405 410 415 Lys Asn Leu Thr Phe Pro Asp Gly Tyr
Val Ile Thr Lys Ile Thr Phe 420 425 430 Glu Lys Lys Leu Asn Asn Leu
Ile Tyr Glu Ala Thr Ala Asn Phe Tyr 435 440 445 Asp Pro Ser Thr Gly
Asp Ile Asp Leu Asn Lys Lys Gln Val Glu Ser 450 455 460 Thr Phe Pro
Gln Thr Asp Tyr Ile Thr Met Asp Ile Gly Asp Asp Asp 465 470 475 480
Gly Ile Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro 485
490 495 Ile Asn Ser Phe Gly Leu Glu Val Asp Ala Lys Ser Lys Thr Leu
Thr 500 505 510 Leu Lys Cys Lys Ser Tyr Leu Arg Glu Tyr Leu Leu Glu
Ser Asp Leu 515 520 525 Lys Asn Lys Glu Thr Gly Leu Ile Ala Pro Pro
Asn Val Phe Ile Ser 530 535 540 Asn Val Val Lys Asn Trp Asp Ile Glu
Glu Asp Ser Leu Glu Pro Trp 545 550 555 560 Val Ala Asn Asn Lys Asn
Ala Tyr Val Asp Asn Thr Gly Gly Ile Glu 565 570 575 Arg Ser Lys Ala
Leu Phe Thr Gln Gly Asp Gly Glu Phe Ser Gln Phe 580 585 590 Ile Gly
Asp Lys Leu Lys Pro Asn Thr Asp Tyr Ile Ile Gln Tyr Thr 595 600 605
Val Lys Gly Lys Pro Ala Ile Tyr Leu Lys Asn Lys Ser Thr Gly Tyr 610
615 620 Ile Thr Tyr Glu Asp Thr Asn Gly Asn Ser Glu Glu Phe Gln Thr
Ile 625 630 635 640 Ala Val Lys Phe Thr Ser Glu Thr Asp Leu Ser Gln
Thr His Leu Val 645 650 655 Phe Lys Ser Gln Asn Gly Tyr Glu Ala Trp
Gly Asp Asn Phe Ile Ile 660 665 670 Leu Glu Ala Lys Leu Phe Glu Thr
Pro Glu Ser Pro Glu Leu Ile Lys 675 680 685 Phe Asn Asp Trp Glu Arg
Phe Gly Thr Thr Tyr Ile Thr Gly Asn Glu 690 695 700 Leu Arg Ile Asp
His Ser Arg Gly Gly Tyr Phe Arg Gln Ser Leu Asn 705 710 715 720 Ile
Asp Ser Tyr Ser Thr Tyr Asp Leu Ser Phe Ser Phe Ser Gly Leu 725 730
735 Trp Ala Lys Val Ile Val Lys Asn Ser Arg Gly Val Val Leu Phe Glu
740 745 750 Lys Val Lys Asn Asn Gly Ser Ser Tyr Glu Asp Ile Ser Glu
Ser Phe 755 760 765 Thr Thr Met Ser Asn Lys Asp Gly Phe Phe Ile Glu
Leu Thr Ala Glu 770 775 780 Arg Thr Ser Ser Thr Phe His Ser Phe Arg
Asp Ile Ser Ile Lys Glu 785 790 795 800 Lys Ile Glu
543468DNABacillus thuringiensis 54atgaatcgaa ataatcaaaa tgaatgtgaa
attattgatg cccctcattg tggatgtccg 60tcagatgatg ttgtgaaata tcctttggca
agtgacccaa atgcagcgtt acaaaatatg 120aactataaag attatttaca
aacgtatgat ggagactata cagattctct tattaatcct 180aacttatcta
ttaatactag ggatgtacta caaacaggta ttactattgt gggaagaata
240ctagggtttt taggtgttcc atttgcgggg caactagtta ctttctatac
ctttctctta 300aatcagttat ggccaactaa tgataatgca gtatgggaag
cttttatgga acaaatagaa 360gggattatcg ctcaaagaat atcggagcaa
gtagtaagga atgcgcttga tgccttaact 420ggaatacacg attattatga
ggaatattta gcggcattag aggagtggct ggaaagaccg 480agcggcgcaa
gggctaactt agcttttcag aggtttgaaa atctacatca attatttgta
540agtcagatgc caagttttgg tagtggtcct ggtagtgaaa gagatgcggt
agcattgctg 600acagtatatg cacaagcagc gaatctccat ttgttgttat
taaaagatgc agaaatttat 660ggggcgagat ggggacttca acaaggccaa
attaatttat attttaatgc tcaacaagat 720cgcactcgaa tttataccaa
tcattgtgtg gcaacatata atagaggatt aggagactta 780agaggcacaa
atactgaaag ttggttaaat taccatcaat tccgtagaga gatgacatta
840atggcaatgg atttagtggc attattccca tactataatt tacgacaata
tccaaacggg 900gcaaaccctc agcttacacg tgatgtatat acagatccga
ttgtatttaa tccatcagct 960aatgtaggat tatgtagacg ttggggcaat
aacccatata atacattttc ggaacttgaa 1020aatgccttca ttcgcccgcc
acattttttt gataggttga atagtttaac aattagtaga 1080aatagatttg
acgttggatc aaactttata gagccttggt ctggacatac gttacgccgt
1140agttttctga acacttcggc agtacaagaa gatagttatg gccaaattac
taatcaaaga 1200acaacaatta atctaccagc taatggaact gggcgagtgg
agtcaacagc agtagatttt 1260cgtagcgcgc ttgtggggat atacggcgtt
aatagagctt cttttattcc cggtggtgtg 1320tttaatggca cgactcaacc
ttctactgga ggatgtagag atttgtatga ttcaagtgat 1380gaattaccac
cagaagaaag tagtggaacg tttgaacata ggttatctca tgttaccttt
1440ttaagtttta caactaatca ggctggatcc atagccaatg cagggcgcgt
ccctacttat 1500gtctggaccc atcgagatgt ggaccttaat aacacgatta
ctgcagatag aattacacac 1560ttaccattga taaaatcaaa tgtgcaacgc
agtggtcgcg cagtaaaagg accaggattt 1620acaggaggag atgtactccg
aatgtcatca agtgatgctg atatatcaat aataggaata 1680acggcaggtg
caccgctaac acaacaatat cgtataagat tgcgttatgc ttcaaatgta
1740gatgttacta tccgtttagt gagacaggac acccaaagta atataggaag
cataaactta 1800ttacgtacaa tgaacagtgg agaggagtca aggtatgaat
catatcgtac tgtagagatg 1860cctggtaatt ttagaatgac tagtagttca
gcacagattc gactatttac tcaaggactt 1920cgagtgaatg gagaattgtt
tcttgatagt cttgaattta tcccagttaa tccgacacgt 1980gaggcggaag
aggatttaga agcagcgaag aaagcggtga cgagcttgtt tacacgtaca
2040agtgatggat tacagataaa tgtgacagat taccaagtcg atcaggcggc
aaatttagtg 2100tcgtgcttat cagatgaaca atatgggcat gataaaaaga
tgttattgga agccgtacgc 2160gcagcaaaac gcctcagccg cgaacgcaac
ttacttcaag atccagattt taatacaatc 2220aatagtacag aagaaaatgg
ctggaaggca agtaacggtg ttactattag cgagggcggt 2280ccattcttta
aaggtcgtgc acttcagtta gcaagcgcaa gagaaaatta tccaacatac
2340atttatcaaa aagtagatgc atcggtgtta aagccttata cacgctatag
actagatgga 2400tttgtgaaga gtagtcaaga tttagaaatt gatctcatcc
accatcataa agtccatctt 2460gtaaaaaatg taccagataa tttagtatct
gatacttact cagatggttc ttgcagcgga 2520atcaaccgtt gtgatgaaca
gcagcaggta gatatgcagc tagatgcgga gcatcatcca 2580atggattgct
gtgaagtggc tcaaacacat gagttttctt cctatattaa tacaggggat
2640ctaaatgcaa gtgtagatca gggcatttgg gttgtattaa aagttcgaac
aacagatggg 2700tatgcgacgt taggaaatct tgaattggta gaggttgggc
cattatcggg tgaatctcta 2760gaacgcgaac aaagagataa tgcgaaatgg
aatgcagagc taggaagaaa gcgtgcagaa 2820acagatcgcg tgtatctagc
tgcgaaacaa gcaattaatc atctatttgt agactatcaa 2880gatcaacaat
taaatccaga aattgggcta gcggaaataa atgaagcttc aaatcttgtg
2940aagtcaattt cgggtgtata tagtgataca ctattacaga ttcctggaat
taactacgaa 3000atttacacag agttatccga tcgattacaa caagcatcgt
atctgtatac gtctcgaaat 3060gccgtgcaaa atggagactt taacagtggt
ctagatagtt ggaatgcaac aacagatgca 3120tcggttcagc aagatggcag
tacacatttc ttagttcttt cgcattggga tgcacaagtt 3180tcccaacaaa
tgagagtaaa tttgaattgt aagtatgttt tacgtgtaac agcaaaaaaa
3240gtaggaggcg gagatggata cgtcacaatc cgagatggcg ctcatcacca
agaaactctt 3300acatttaatg catgtgacta cgatgtaaat ggtacgtatg
tcaatgacaa ttcgtacata 3360acaaaagaag tggtattcta cccagagaca
aaacatatgt gggtagaggt gagtgaatcc 3420gaaggttcat tctatataga
cagtattgag tttattgaaa cacaagag 3468552037DNAArtificial
Sequencesynthetic gene encoding toxin protein 55atgaacagga
acaaccaaaa tgaatgtgag atcatcgacg cgccgcactg cggctgccct 60tctgatgatg
tggtgaagta cccgctggct tcagatccaa atgctgctct ccagaacatg
120aactacaagg actacctcca gacatatgat ggagactaca ccgacagcct
catcaacccc 180aacctcagca tcaacacaag agatgtgctg caaactggca
tcaccatcgt cggcaggatc 240ttgggcttcc tcggcgtgcc cttcgccggc
cagctggtga ccttctacac cttcctcctc 300aaccagctat ggccaacaaa
tgacaatgct gtttgggagg ccttcatgga gcagattgaa 360ggcatcattg
ctcaaaggat
ctccgagcaa gtggtgagga atgctcttga tgctctcacc 420ggcatccatg
actactacga ggagtacctg gcggcgctgg aggagtggct ggagaggcca
480tcaggcgcgc gcgccaacct cgccttccaa agatttgaga acctccacca
gctcttcgtg 540agccagatgc cgagcttcgg cagcggccct ggatcagaaa
gagatgctgt ggcgctgctc 600accgtctatg ctcaagctgc caacctccat
ctgctgctgc tgaaggatgc tgagatctat 660ggagcaagat ggggcctcca
gcaagggcag atcaacctct acttcaatgc tcaacaagac 720aggacaagga
tctacaccaa ccactgcgtg gcaacataca accgcggcct cggcgacctc
780cgcggcacca acactgaatc atggctcaac taccaccagt tcagaaggga
gatgacattg 840atggccatgg acctggtggc gctcttcccc tactacaacc
tccgccaata tcctaatgga 900gcaaatcctc agctgacaag agatgtctac
accgacccca tcgtcttcaa cccttcagca 960aatgttggcc tctgccgccg
ctggggcaac aatccttaca acaccttctc cgagctggag 1020aatgccttca
tcaggccgcc gcacttcttc gaccgcctca acagcctcac catctcaagg
1080aacagatttg atgttggaag caacttcatc gagccatgga gcggccacac
cttgaggagg 1140agcttcctca acacctccgc cgtccaagaa gacagctatg
gccagatcac caaccagagg 1200accaccatca acctaccagc aaatggaact
ggaagagttg agagcaccgc cgtggacttc 1260cgctcggcgc tggtgggcat
ctacggcgtg aacagggcca gcttcatccc cggcggcgtc 1320ttcaatggaa
caactcaacc atcaacagga ggctgccgag atctttatga ttcttctgat
1380gagctgccgc cggaggagag cagcggcacc ttcgagcacc gcctcagcca
tgtcaccttc 1440ttgagcttca ccaccaacca agctggatca attgcaaatg
ctggaagggt gccaacatat 1500gtttggaccc accgtgatgt ggacctcaac
aacaccatca ccgccgacag gatcacccat 1560ctgccgctca tcaagagcaa
tgttcaaaga tcaggccgcg ccgtcaaagg acctggcttc 1620accggcggcg
acgtgctgag gatgagcagc tctgatgctg acatcagcat catcggcatc
1680accgccggcg ctccgctgac tcagcagtac aggatcaggc tgagatatgc
ttcaaatgtt 1740gatgtcacca tcaggctggt gcggcaagac acccagagca
acatcggcag catcaacctg 1800ctgaggacaa tgaacagtgg agaagaaagc
agatatgaaa gctacaggac ggtggagatg 1860cctggcaact tcagaatgac
atcaagctcg gcgcagatca ggctcttcac ccaaggcctc 1920cgcgtcaatg
gagagctctt cctggacagc ttggagttca tccccgtcaa cccaacaaga
1980gaagcagaag aagatctgga ggccgccaag aaggcggtga caagcctctt cacaaga
2037561156PRTBacillus thuringiensis 56Met Asn Arg Asn Asn Gln Asn
Glu Cys Glu Ile Ile Asp Ala Pro His 1 5 10 15 Cys Gly Cys Pro Ser
Asp Asp Val Val Lys Tyr Pro Leu Ala Ser Asp 20 25 30 Pro Asn Ala
Ala Leu Gln Asn Met Asn Tyr Lys Asp Tyr Leu Gln Thr 35 40 45 Tyr
Asp Gly Asp Tyr Thr Asp Ser Leu Ile Asn Pro Asn Leu Ser Ile 50 55
60 Asn Thr Arg Asp Val Leu Gln Thr Gly Ile Thr Ile Val Gly Arg Ile
65 70 75 80 Leu Gly Phe Leu Gly Val Pro Phe Ala Gly Gln Leu Val Thr
Phe Tyr 85 90 95 Thr Phe Leu Leu Asn Gln Leu Trp Pro Thr Asn Asp
Asn Ala Val Trp 100 105 110 Glu Ala Phe Met Glu Gln Ile Glu Gly Ile
Ile Ala Gln Arg Ile Ser 115 120 125 Glu Gln Val Val Arg Asn Ala Leu
Asp Ala Leu Thr Gly Ile His Asp 130 135 140 Tyr Tyr Glu Glu Tyr Leu
Ala Ala Leu Glu Glu Trp Leu Glu Arg Pro 145 150 155 160 Ser Gly Ala
Arg Ala Asn Leu Ala Phe Gln Arg Phe Glu Asn Leu His 165 170 175 Gln
Leu Phe Val Ser Gln Met Pro Ser Phe Gly Ser Gly Pro Gly Ser 180 185
190 Glu Arg Asp Ala Val Ala Leu Leu Thr Val Tyr Ala Gln Ala Ala Asn
195 200 205 Leu His Leu Leu Leu Leu Lys Asp Ala Glu Ile Tyr Gly Ala
Arg Trp 210 215 220 Gly Leu Gln Gln Gly Gln Ile Asn Leu Tyr Phe Asn
Ala Gln Gln Asp 225 230 235 240 Arg Thr Arg Ile Tyr Thr Asn His Cys
Val Ala Thr Tyr Asn Arg Gly 245 250 255 Leu Gly Asp Leu Arg Gly Thr
Asn Thr Glu Ser Trp Leu Asn Tyr His 260 265 270 Gln Phe Arg Arg Glu
Met Thr Leu Met Ala Met Asp Leu Val Ala Leu 275 280 285 Phe Pro Tyr
Tyr Asn Leu Arg Gln Tyr Pro Asn Gly Ala Asn Pro Gln 290 295 300 Leu
Thr Arg Asp Val Tyr Thr Asp Pro Ile Val Phe Asn Pro Ser Ala 305 310
315 320 Asn Val Gly Leu Cys Arg Arg Trp Gly Asn Asn Pro Tyr Asn Thr
Phe 325 330 335 Ser Glu Leu Glu Asn Ala Phe Ile Arg Pro Pro His Phe
Phe Asp Arg 340 345 350 Leu Asn Ser Leu Thr Ile Ser Arg Asn Arg Phe
Asp Val Gly Ser Asn 355 360 365 Phe Ile Glu Pro Trp Ser Gly His Thr
Leu Arg Arg Ser Phe Leu Asn 370 375 380 Thr Ser Ala Val Gln Glu Asp
Ser Tyr Gly Gln Ile Thr Asn Gln Arg 385 390 395 400 Thr Thr Ile Asn
Leu Pro Ala Asn Gly Thr Gly Arg Val Glu Ser Thr 405 410 415 Ala Val
Asp Phe Arg Ser Ala Leu Val Gly Ile Tyr Gly Val Asn Arg 420 425 430
Ala Ser Phe Ile Pro Gly Gly Val Phe Asn Gly Thr Thr Gln Pro Ser 435
440 445 Thr Gly Gly Cys Arg Asp Leu Tyr Asp Ser Ser Asp Glu Leu Pro
Pro 450 455 460 Glu Glu Ser Ser Gly Thr Phe Glu His Arg Leu Ser His
Val Thr Phe 465 470 475 480 Leu Ser Phe Thr Thr Asn Gln Ala Gly Ser
Ile Ala Asn Ala Gly Arg 485 490 495 Val Pro Thr Tyr Val Trp Thr His
Arg Asp Val Asp Leu Asn Asn Thr 500 505 510 Ile Thr Ala Asp Arg Ile
Thr His Leu Pro Leu Ile Lys Ser Asn Val 515 520 525 Gln Arg Ser Gly
Arg Ala Val Lys Gly Pro Gly Phe Thr Gly Gly Asp 530 535 540 Val Leu
Arg Met Ser Ser Ser Asp Ala Asp Ile Ser Ile Ile Gly Ile 545 550 555
560 Thr Ala Gly Ala Pro Leu Thr Gln Gln Tyr Arg Ile Arg Leu Arg Tyr
565 570 575 Ala Ser Asn Val Asp Val Thr Ile Arg Leu Val Arg Gln Asp
Thr Gln 580 585 590 Ser Asn Ile Gly Ser Ile Asn Leu Leu Arg Thr Met
Asn Ser Gly Glu 595 600 605 Glu Ser Arg Tyr Glu Ser Tyr Arg Thr Val
Glu Met Pro Gly Asn Phe 610 615 620 Arg Met Thr Ser Ser Ser Ala Gln
Ile Arg Leu Phe Thr Gln Gly Leu 625 630 635 640 Arg Val Asn Gly Glu
Leu Phe Leu Asp Ser Leu Glu Phe Ile Pro Val 645 650 655 Asn Pro Thr
Arg Glu Ala Glu Glu Asp Leu Glu Ala Ala Lys Lys Ala 660 665 670 Val
Thr Ser Leu Phe Thr Arg Thr Ser Asp Gly Leu Gln Ile Asn Val 675 680
685 Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser
690 695 700 Asp Glu Gln Tyr Gly His Asp Lys Lys Met Leu Leu Glu Ala
Val Arg 705 710 715 720 Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu
Leu Gln Asp Pro Asp 725 730 735 Phe Asn Thr Ile Asn Ser Thr Glu Glu
Asn Gly Trp Lys Ala Ser Asn 740 745 750 Gly Val Thr Ile Ser Glu Gly
Gly Pro Phe Phe Lys Gly Arg Ala Leu 755 760 765 Gln Leu Ala Ser Ala
Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 770 775 780 Val Asp Ala
Ser Val Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 785 790 795 800
Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 805
810 815 Lys Val His Leu Val Lys Asn Val Pro Asp Asn Leu Val Ser Asp
Thr 820 825 830 Tyr Ser Asp Gly Ser Cys Ser Gly Ile Asn Arg Cys Asp
Glu Gln Gln 835 840 845 Gln Val Asp Met Gln Leu Asp Ala Glu His His
Pro Met Asp Cys Cys 850 855 860 Glu Val Ala Gln Thr His Glu Phe Ser
Ser Tyr Ile Asn Thr Gly Asp 865 870 875 880 Leu Asn Ala Ser Val Asp
Gln Gly Ile Trp Val Val Leu Lys Val Arg 885 890 895 Thr Thr Asp Gly
Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Val 900 905 910 Gly Pro
Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 915 920 925
Lys Trp Asn Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 930
935 940 Tyr Leu Ala Ala Lys Gln Ala Ile Asn His Leu Phe Val Asp Tyr
Gln 945 950 955 960 Asp Gln Gln Leu Asn Pro Glu Ile Gly Leu Ala Glu
Ile Asn Glu Ala 965 970 975 Ser Asn Leu Val Lys Ser Ile Ser Gly Val
Tyr Ser Asp Thr Leu Leu 980 985 990 Gln Ile Pro Gly Ile Asn Tyr Glu
Ile Tyr Thr Glu Leu Ser Asp Arg 995 1000 1005 Leu Gln Gln Ala Ser
Tyr Leu Tyr Thr Ser Arg Asn Ala Val Gln 1010 1015 1020 Asn Gly Asp
Phe Asn Ser Gly Leu Asp Ser Trp Asn Ala Thr Thr 1025 1030 1035 Asp
Ala Ser Val Gln Gln Asp Gly Ser Thr His Phe Leu Val Leu 1040 1045
1050 Ser His Trp Asp Ala Gln Val Ser Gln Gln Met Arg Val Asn Leu
1055 1060 1065 Asn Cys Lys Tyr Val Leu Arg Val Thr Ala Lys Lys Val
Gly Gly 1070 1075 1080 Gly Asp Gly Tyr Val Thr Ile Arg Asp Gly Ala
His His Gln Glu 1085 1090 1095 Thr Leu Thr Phe Asn Ala Cys Asp Tyr
Asp Val Asn Gly Thr Tyr 1100 1105 1110 Val Asn Asp Asn Ser Tyr Ile
Thr Lys Glu Val Val Phe Tyr Pro 1115 1120 1125 Glu Thr Lys His Met
Trp Val Glu Val Ser Glu Ser Glu Gly Ser 1130 1135 1140 Phe Tyr Ile
Asp Ser Ile Glu Phe Ile Glu Thr Gln Glu 1145 1150 1155
571902DNABacillus thuringiensisCDS(1)..(1902) 57atg aat aat gta tta
aat aac gga aga act act aat tgt gat gcg tat 48Met Asn Asn Val Leu
Asn Asn Gly Arg Thr Thr Asn Cys Asp Ala Tyr 1 5 10 15 aat gta gtg
gcc cat gat cca ttt agt ttt gag cat aaa tca tta gat 96Asn Val Val
Ala His Asp Pro Phe Ser Phe Glu His Lys Ser Leu Asp 20 25 30 acc
atc cga aaa gaa tgg atg gag tgg aaa aga aca gat cat agt tta 144Thr
Ile Arg Lys Glu Trp Met Glu Trp Lys Arg Thr Asp His Ser Leu 35 40
45 tat gta gct cct gta gtc gga act gtt tct agc ttt ctg cta aag aag
192Tyr Val Ala Pro Val Val Gly Thr Val Ser Ser Phe Leu Leu Lys Lys
50 55 60 gtg ggg agt ctt att gga aaa agg ata ttg agt gaa tta tgg
ggg tta 240Val Gly Ser Leu Ile Gly Lys Arg Ile Leu Ser Glu Leu Trp
Gly Leu 65 70 75 80 ata ttt cct agt ggt agc aca aat cta atg caa gat
att tta agg gag 288Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp
Ile Leu Arg Glu 85 90 95 aca gaa caa ttc cta aat caa aga ctt aat
aca gac act ctt gct cgt 336Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn
Thr Asp Thr Leu Ala Arg 100 105 110 gta aat gcg gaa ttg aca ggg ctg
caa gcg aat ata agg gag ttt aat 384Val Asn Ala Glu Leu Thr Gly Leu
Gln Ala Asn Ile Arg Glu Phe Asn 115 120 125 caa caa gta gat aat ttt
tta aat cct act caa aac cct gtt cct tta 432Gln Gln Val Asp Asn Phe
Leu Asn Pro Thr Gln Asn Pro Val Pro Leu 130 135 140 tca ata act tct
tca gtt aat aca atg cag caa tta ttt cta aat aga 480Ser Ile Thr Ser
Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn Arg 145 150 155 160 tta
ccc cag ttc cag ata caa gga tac caa ctg tta tta tta cct tta 528Leu
Pro Gln Phe Gln Ile Gln Gly Tyr Gln Leu Leu Leu Leu Pro Leu 165 170
175 ttt gca cag gca gcc aat atg cat ctt tct ttt att aga gat gtt att
576Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val Ile
180 185 190 ctt aat gca gat gaa tgg gga att tca gca gca aca cta cgt
acg tat 624Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg
Thr Tyr 195 200 205 cga gac tac ctg aga aat tat aca aga gat tat tct
aat tat tgt ata 672Arg Asp Tyr Leu Arg Asn Tyr Thr Arg Asp Tyr Ser
Asn Tyr Cys Ile 210 215 220 aat acg tat caa act gcg ttt aga ggg tta
aac acc cgt tta cac gat 720Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu
Asn Thr Arg Leu His Asp 225 230 235 240 atg tta gaa ttt aga aca tat
atg ttt tta aat gta ttt gaa tat gta 768Met Leu Glu Phe Arg Thr Tyr
Met Phe Leu Asn Val Phe Glu Tyr Val 245 250 255 tcc att tgg tca ttg
ttt aaa tat cag agt ctt atg gta tct tct ggc 816Ser Ile Trp Ser Leu
Phe Lys Tyr Gln Ser Leu Met Val Ser Ser Gly 260 265 270 gct aat tta
tat gct agt ggt agt gga cca cag cag acc caa tca ttt 864Ala Asn Leu
Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser Phe 275 280 285 att
tca caa gac tgg cca ttt tta tat tct ctt ttc caa gtt aat tca 912Ile
Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn Ser 290 295
300 aat tat gtg tta aat ggc ttt agt ggc gct agg aat act att aga ttc
960Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Asn Thr Ile Arg Phe
305 310 315 320 cca gct ggt ggt ggt tta tta cca cct ggt gtt act aca
act cac gca 1008Pro Ala Gly Gly Gly Leu Leu Pro Pro Gly Val Thr Thr
Thr His Ala 325 330 335 ttg ctt gct gca agg gtc aat tac agt gga gga
gtt tcg tct ggt tat 1056Leu Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly
Val Ser Ser Gly Tyr 340 345 350 ata ggc gct gtg ttt aat caa aat ttt
aat tgt agc aca ctt ctc cca 1104Ile Gly Ala Val Phe Asn Gln Asn Phe
Asn Cys Ser Thr Leu Leu Pro 355 360 365 cct ttg tta aca cca ttt gtt
agg agt tgg cta gat tca ggt aca gat 1152Pro Leu Leu Thr Pro Phe Val
Arg Ser Trp Leu Asp Ser Gly Thr Asp 370 375 380 cgg gag ggc gtt acc
acc gtt aca aat tgg caa aca gaa tcc ttt aag 1200Arg Glu Gly Val Thr
Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Lys 385 390 395 400 aca act
tta ggt tta agg tgt ggt gct ttt aca ccc cgt ggt aat tca 1248Thr Thr
Leu Gly Leu Arg Cys Gly Ala Phe Thr Pro Arg Gly Asn Ser 405 410 415
aac tat ttc cca gat tat ttt atc cgt aat att tct ggc gtt cct tta
1296Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430 gtt gtt aga aac gaa gat tta aga aga ccg tta cac tat aat
gaa ata 1344Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn
Glu Ile 435 440 445 aga aat ata gaa agt ccc tca gga aca cct ggt gga
tta cga gct tat 1392Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly
Leu Arg Ala Tyr 450 455 460 atg gta tct gtg cat aac aga aaa aat aat
atc tat gcc gct cat gaa 1440Met Val Ser Val His Asn Arg Lys Asn Asn
Ile Tyr Ala Ala His Glu 465 470 475 480 aat ggt act atg att cat ttg
gca ccg gaa gat tat aca gga ttt act 1488Asn Gly Thr Met Ile His Leu
Ala Pro Glu Asp Tyr Thr Gly Phe Thr 485 490 495 atg ttg ccg ata cat
gca act caa gtg aat aat caa acg cga aca ttt 1536Met Leu Pro Ile His
Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe 500
505 510 att tct gaa aaa ttt gga aat caa ggt gat tcc tta aga ttt gaa
caa 1584Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu
Gln 515 520 525 agc gac acg aca gct cgt tat aca ctt aga ggg aat gga
aat agt tac 1632Ser Asp Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly
Asn Ser Tyr 530 535 540 aat ctt tat tta aga gta tct tct cta gga aat
tcc act att cga gtt 1680Asn Leu Tyr Leu Arg Val Ser Ser Leu Gly Asn
Ser Thr Ile Arg Val 545 550 555 560 act ata aac gga aga gtt tat act
gtt cca aat gtt aat aca aat ata 1728Thr Ile Asn Gly Arg Val Tyr Thr
Val Pro Asn Val Asn Thr Asn Ile 565 570 575 aat aac gat gga gtc att
gat aat gga gct cgt ttt tca gat att aat 1776Asn Asn Asp Gly Val Ile
Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn 580 585 590 atc ggt aat gta
gta gca agt gat aat act aat gta ccg tta gat ata 1824Ile Gly Asn Val
Val Ala Ser Asp Asn Thr Asn Val Pro Leu Asp Ile 595 600 605 aac gtg
aca tta agt tct gga act caa ttt gag ctt atg aat att atg 1872Asn Val
Thr Leu Ser Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met 610 615 620
ttt gtt cca act aat ctt cca cca ata tat 1902Phe Val Pro Thr Asn Leu
Pro Pro Ile Tyr 625 630 58634PRTBacillus thuringiensis 58Met Asn
Asn Val Leu Asn Asn Gly Arg Thr Thr Asn Cys Asp Ala Tyr 1 5 10 15
Asn Val Val Ala His Asp Pro Phe Ser Phe Glu His Lys Ser Leu Asp 20
25 30 Thr Ile Arg Lys Glu Trp Met Glu Trp Lys Arg Thr Asp His Ser
Leu 35 40 45 Tyr Val Ala Pro Val Val Gly Thr Val Ser Ser Phe Leu
Leu Lys Lys 50 55 60 Val Gly Ser Leu Ile Gly Lys Arg Ile Leu Ser
Glu Leu Trp Gly Leu 65 70 75 80 Ile Phe Pro Ser Gly Ser Thr Asn Leu
Met Gln Asp Ile Leu Arg Glu 85 90 95 Thr Glu Gln Phe Leu Asn Gln
Arg Leu Asn Thr Asp Thr Leu Ala Arg 100 105 110 Val Asn Ala Glu Leu
Thr Gly Leu Gln Ala Asn Ile Arg Glu Phe Asn 115 120 125 Gln Gln Val
Asp Asn Phe Leu Asn Pro Thr Gln Asn Pro Val Pro Leu 130 135 140 Ser
Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn Arg 145 150
155 160 Leu Pro Gln Phe Gln Ile Gln Gly Tyr Gln Leu Leu Leu Leu Pro
Leu 165 170 175 Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg
Asp Val Ile 180 185 190 Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala
Thr Leu Arg Thr Tyr 195 200 205 Arg Asp Tyr Leu Arg Asn Tyr Thr Arg
Asp Tyr Ser Asn Tyr Cys Ile 210 215 220 Asn Thr Tyr Gln Thr Ala Phe
Arg Gly Leu Asn Thr Arg Leu His Asp 225 230 235 240 Met Leu Glu Phe
Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr Val 245 250 255 Ser Ile
Trp Ser Leu Phe Lys Tyr Gln Ser Leu Met Val Ser Ser Gly 260 265 270
Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser Phe 275
280 285 Ile Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
Ser 290 295 300 Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Asn Thr
Ile Arg Phe 305 310 315 320 Pro Ala Gly Gly Gly Leu Leu Pro Pro Gly
Val Thr Thr Thr His Ala 325 330 335 Leu Leu Ala Ala Arg Val Asn Tyr
Ser Gly Gly Val Ser Ser Gly Tyr 340 345 350 Ile Gly Ala Val Phe Asn
Gln Asn Phe Asn Cys Ser Thr Leu Leu Pro 355 360 365 Pro Leu Leu Thr
Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Thr Asp 370 375 380 Arg Glu
Gly Val Thr Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Lys 385 390 395
400 Thr Thr Leu Gly Leu Arg Cys Gly Ala Phe Thr Pro Arg Gly Asn Ser
405 410 415 Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val
Pro Leu 420 425 430 Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His
Tyr Asn Glu Ile 435 440 445 Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro
Gly Gly Leu Arg Ala Tyr 450 455 460 Met Val Ser Val His Asn Arg Lys
Asn Asn Ile Tyr Ala Ala His Glu 465 470 475 480 Asn Gly Thr Met Ile
His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr 485 490 495 Met Leu Pro
Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe 500 505 510 Ile
Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln 515 520
525 Ser Asp Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540 Asn Leu Tyr Leu Arg Val Ser Ser Leu Gly Asn Ser Thr Ile
Arg Val 545 550 555 560 Thr Ile Asn Gly Arg Val Tyr Thr Val Pro Asn
Val Asn Thr Asn Ile 565 570 575 Asn Asn Asp Gly Val Ile Asp Asn Gly
Ala Arg Phe Ser Asp Ile Asn 580 585 590 Ile Gly Asn Val Val Ala Ser
Asp Asn Thr Asn Val Pro Leu Asp Ile 595 600 605 Asn Val Thr Leu Ser
Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met 610 615 620 Phe Val Pro
Thr Asn Leu Pro Pro Ile Tyr 625 630 591902DNAArtificial
SequenceSynthetic sequence encoding AXMI-076 (optaxmi- 076)
59atgaacaatg tgctcaacaa tggaagaaca acaaattgtg atgcctacaa tgtggtggct
60catgatccct tcagcttcga gcacaagagc ttggacacca tcaggaagga gtggatggaa
120tggaagagga cagatcattc tctttatgtg gcgccggtgg tgggcaccgt
ctcatccttc 180ctgctgaaga aggtgggcag cctcatcggc aagaggattc
tttctgagct ctggggcctc 240atcttcccaa gtggatcaac aaatttgatg
caagacatcc tccgagaaac agagcagttc 300ctcaatcaaa ggctcaacac
cgacaccttg gcaagggtga atgctgagct gacagggcta 360caagccaaca
tcagggagtt caaccagcag gtggacaact tcctcaaccc aactcaaaat
420ccagtgccgc tgagcatcac ctcctctgtt aacaccatgc agcagctctt
cctcaacagg 480ctgccgcagt tccagatcca aggctaccag ctgctgctgc
tgccgctatt tgctcaagct 540gccaacatgc acctcagctt catcagagat
gtcatcctca atgctgatga atggggcatc 600tcggcggcga cgctgaggac
ctacagagat tatttgagga actacacaag ggactactca 660aactactgca
tcaacaccta ccagacggcc ttccgcggcc tcaacacaag gctgcatgac
720atgctggagt tcagaaccta catgttcctc aatgtttttg aatatgtttc
aatttggagc 780ctcttcaagt accagagctt gatggtgagc agcggcgcca
acctctatgc ttctggaagt 840gggccgcagc aaactcagag cttcatcagc
caagattggc ccttcctcta cagcctcttc 900caagttaaca gcaactacgt
gctgaatggc ttctcaggag caaggaacac catcagattt 960cctgctggag
gagggctgct gccgccgggc gtgacaacaa ctcatgctct gctggcggca
1020agagttaact actctggagg agtttcaagc ggctacatcg gcgccgtctt
caaccagaac 1080ttcaactgct cgacgctgct gccgccgctg ctgacgccat
ttgtgaggag ctggctggac 1140agcggcaccg acagagaagg agtcaccacc
gtcaccaact ggcaaactga gagcttcaag 1200acgacgctgg gcctccgctg
cggcgccttc acgccgcgcg gcaactcaaa ctacttcccg 1260gactacttca
tcaggaacat cagcggcgtg ccgctggtgg tgaggaatga agatttgagg
1320aggccgctgc actacaatga gatcaggaac atcgagagcc catcaggaac
tcctggaggc 1380ctccgcgcct acatggtttc tgttcacaac aggaagaaca
acatctatgc tgctcatgaa 1440aatggcacca tgatccacct ggcgccggag
gactacaccg gcttcaccat gctacccatc 1500catgcaactc aagtcaacaa
ccaaacaagg accttcatct ccgagaagtt tggaaatcaa 1560ggagattctt
tgagatttga gcagagcgac accacggcgc gctacacctt gagaggaaat
1620ggcaacagct acaacctcta cctccgcgtc agcagcctcg gcaactcaac
catcagggtg 1680accatcaatg gccgcgtcta caccgtgcca aatgtcaaca
ccaacatcaa caatgatggc 1740gtcatcgaca atggagcaag attctccgac
atcaacatcg gcaatgtggt ggcctccgac 1800aacaccaacg tgccgctgga
catcaatgtc accttgagct caggaactca atttgagctg 1860atgaacatca
tgttcgtgcc aacaaatctt cctcccatct ac 1902601902DNAArtificial
SequenceSynthetic sequence encoding AXMI-076 (optaxmi- 076v04)
60atgaacaatg ttctcaacaa tggaagaaca acaaactgtg atgcctacaa tgttgttgct
60catgatcctt tctcatttga acacaagagc ttggacacca tcagaaaaga atggatggaa
120tggaaaagaa cagatcattc tctctatgtt gctcctgttg ttggaactgt
tagcagcttc 180ttgctgaaga aggttggcag cttgattgga aaaaggattc
tttcagagct ctggggcttg 240atctttcctt ctggaagcac caacttgatg
caagacatct tgagagaaac agagcagttc 300ttgaaccaaa ggctcaacac
agacaccttg gcaagggtga atgctgagct cactggcctt 360caagcaaaca
tcagagagtt caaccaacaa gttgacaact tcttgaatcc aacacaaaat
420cctgttcctc tctccatcac ttcttcagtg aacaccatgc agcagctctt
cttgaacagg 480cttcctcagt tccagattca aggatatcag ctgctgctgc
ttcctctctt tgctcaagct 540gcaaacatgc atctctcctt catcagagat
gtcatcttga atgctgatga atggggcatc 600tctgctgcca ccttgagaac
atacagagat tatttgagaa actacacaag agattattca 660aactactgca
tcaacacata tcaaacagct ttcagaggcc tcaacacaag gcttcatgac
720atgctggagt tcagaacata catgttcttg aatgtttttg aatatgtttc
catctggagc 780ttgttcaagt accagagctt gatggtttct tctggagcaa
atttatatgc atctggatct 840ggacctcaac aaacacagag cttcatctct
caagattggc catttctcta cagcttgttc 900caagtgaaca gcaactatgt
tcttaatggc ttctctggag caagaaacac catcagattt 960cctgctggag
gaggcttgct gcctcctggt gtcaccacca cccatgctct tctggcggcg
1020cgcgtcaact actcaggagg agtttcttct ggatacattg gagctgtgtt
caaccaaaac 1080ttcaactgct ccaccttgct gccgccgctg ctgacaccat
ttgtcagaag ctggctggac 1140tcaggaactg acagagaagg agtcaccacc
gtcaccaact ggcaaacaga gagcttcaaa 1200acaaccttgg gcctccgctg
cggcgccttc acgccgcgcg gcaacagcaa ctactttcca 1260gattacttca
tcagaaacat ttctggagtt cctttggtgg tgagaaatga agatctgagg
1320aggcctcttc actacaatga aatcagaaac attgaatctc catcaggaac
tcctggaggc 1380ctccgcgcct acatggtttc tgttcacaac aggaagaaca
acatctatgc tgctcatgaa 1440aatggaacaa tgattcatct tgctccagaa
gattacactg gcttcaccat gctgcccatc 1500catgcaacac aagtcaacaa
ccaaacaagg accttcattt cagaaaaatt tggaaatcaa 1560ggagattctt
tgagatttga acaaagcgac accacagcaa gatacacctt gagaggaaat
1620ggaaacagct acaacttgta cctccgcgtc agcagcttgg gaaacagcac
catcagggtg 1680accatcaatg gaagagtcta cactgttcca aatgtcaaca
ccaacatcaa caatgatggt 1740gtcattgaca atggagcaag attttcagac
atcaacattg gaaatgtggt ggcttctgac 1800aacacaaatg ttcctctgga
catcaatgtc accctttctt ctggaacaca atttgagctg 1860atgaacatca
tgtttgttcc aacaaatctt cctccaatct ac 1902612370DNABacillus
thuringiensis 61atgaatatga ataatactaa attaaacgca agggccctac
cgagttttat tgattatttt 60aatggcattt atggatttgc cactggtatc aaagacatta
tgaatatgat ttttaaaacg 120gatacaggtg gtaatctaac cttagacgaa
atcctaaaga atcagcagtt actaaatgag 180atttctggta aattggatgg
ggtaaatggg agcttaaatg atcttatcgc acagggaaac 240ttaaatacag
aattatctaa ggaaatctta aaaattgcaa atgaacagaa tcaagtctta
300aatgatgtta ataacaaact cgatgcgata aatacgatgc ttcatatata
tctacctaaa 360attacatcta tgttaagtga tgtaatgaag caaaattatg
cgctaagtct gcaaatagaa 420tacttaagta agcaattgca agaaatttct
gataaattag atattattaa cgtaaatgtt 480cttattaact ctacacttac
tgaaattaca cctgcatatc aacggattaa atatgtgaat 540gaaaaatttg
aagaattaac ttttgctaca gaaaccactt taaaagtaaa aaaggatagc
600tcgcctgctg atattcttga tgagttaact gaattaactg aactagcgaa
aagtgttaca 660aaaaatgacg ttgatggttt tgaattttac cttaatacat
tccacgatgt aatggtagga 720aataatttat tcgggcgttc agctttaaaa
actgcttcag aattaattgc taaagaaaat 780gtgaaaacaa gtggcagtga
agtaggaaat gtttataatt tcttaattgt attaacagct 840ctacaagcaa
aagcttttct tactttaaca acatgccgaa aattattagg cttagcagat
900attgattata cttctattat gaatgaacat ttaaataagg aaaaagagga
atttagagta 960aacatccttc ctacactttc taatactttt tctaatccta
attatgcaaa agttaaagga 1020agtgatgaag atgcaaagat gattgtggaa
gctaaaccag gacatgcatt ggttgggttt 1080gaaatgagca atgattcaat
cacagtatta aaagtatatg aggctaagct aaaacaaaat 1140tatcaagttg
ataaggattc cttatcggag gttatttatg gtgatatgga taaattattg
1200tgtccagatc aatctgaaca aatttattat acaaataata tagtatttcc
aaatgaatat 1260gtaattacta aaattgattt tactaagaaa atgaagactt
taagatatga ggtaacagct 1320aattcttatg attcttctac aggagaaatt
gacttaaata agaagaaagt agaatcaagt 1380gaagcggagt ataggacgtt
aagtgctaaa gatgatggag tgtatatgcc gttaggtgtc 1440atcagtgaaa
catttttgac tccgattaat gggtttggcc tccaagctga tgaaaattca
1500agattaatta ctttaacatg taaatcatat ttaagagaac tactgttagc
aacagactta 1560agcaataaag aaactaaatt gatcgtcccg ccaagtggtt
ttattagtaa tattgtagaa 1620aatgggaact tagagggaga aaacttagag
ccgtggatag caaataataa gaatgcgtat 1680gtagatcata caggcggagt
gaatggtact agagctttat atgttcataa ggacggagga 1740ttttcacaat
ttattggaga taagttaaaa ccgaaaactg agtatgtaat ccaatatact
1800gttaaaggaa aaccttctat tcatttaaaa aatgaaaata ctggatatat
tcattatgaa 1860gatacaaata acaatttaga agattatcaa actattacta
aacgttttac tacaggaact 1920gatttaaagg gagtgtattt aattttaaaa
agtcaaaatg gagatgaagc ctggggagat 1980aactttacaa ttttggaaat
tagtccttct gaaaagttat taagtccaga attaatcaat 2040gtaaataatt
ggattcgcac gggatcaact catattagcg gtaatacact cactctctat
2100cagggaggag gaggaaatct aaaacaaaac cttcaattag acagtttttc
aacttataga 2160gtgaattttt ctgtgaccgg agatgctaat gtaaggatta
gaaattctag ggaagtgtta 2220tttgaaaaac gatatatgag cggtgctaaa
gatgtttctg aaattttcac tacaaaattg 2280gggaaagata acttttatat
agagctttct caagggaata atttatatgg tggtcctctt 2340gtaaagttta
acgatgtctc aattaagtaa 2370622388DNAArtificial Sequencesynthetic
nucleotide sequence encoding AXMI- 005 w/c-terminal extension
62atgaacatga acaacaccaa gctcaatgca agggcgctgc cgagcttcat cgactacttc
60aatggcatct atggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc
120gacaccggcg gcaacctcac cttggatgag atcctcaaga accagcagct
gctgaatgag 180atctcaggca agctggacgg cgtcaatgga agcctcaacg
acctcattgc tcaaggcaac 240ctcaacaccg agctgagcaa ggagatcctc
aagattgcaa atgagcagaa ccaggtgctg 300aatgatgtca acaacaagct
ggacgccatc aacaccatgc tgcacatcta cctgccaaag 360atcacctcaa
tgctctctga tgtgatgaag cagaactacg cgctgagcct ccagattgag
420tacctctcaa agcagctgca agagatctcc gacaagctgg acatcatcaa
tgtcaatgtg 480ctcatcaaca gcaccttgac agagatcacg ccggcctacc
agaggatcaa gtatgtcaat 540gagaagtttg aggagctcac cttcgccacc
gagacaacat tgaaggtgaa gaaggacagc 600tcgccggcgg acatcctgga
tgagctcacc gagctaacag agctggccaa gagcgtcacc 660aagaatgatg
ttgatggctt cgagttctac ctcaacacct tccatgatgt gatggtgggc
720aacaacctct tcggccgctc ggcgctcaag acggcgtcgg agctgatcgc
caaggagaat 780gtcaagacaa gtggatcaga ggtgggcaat gtctacaact
tcctcatcgt gctgacggcg 840ctgcaagcca aggccttcct caccttgaca
acctgccgca agttgctggg cctcgccgac 900atcgactaca cctccatcat
gaatgagcac ctcaacaagg agaaggagga gttccgcgtc 960aacatcctgc
caacattgag caacaccttc agcaacccca actacgccaa ggtgaagggc
1020tcagatgaag atgccaagat gattgtggag gccaagcctg gccatgctct
ggtgggcttc 1080gagatgagca acgacagcat caccgtgctg aaggtctacg
aggccaagct gaagcagaac 1140taccaggtgg acaaggacag cttgtctgag
gtgatctacg gcgacatgga caagctgcta 1200tgtccagatc aaagcgagca
gatctactac accaacaaca tcgtctttcc aaatgaatat 1260gtcatcacca
agatcgactt caccaagaag atgaaaacat tgagatatga ggtgacggcc
1320aacagctacg acagcagcac cggcgagatc gacctcaaca agaagaaggt
ggagagctca 1380gaagctgagt acaggacgct ctccgccaag gatgatggcg
tctacatgcc gctcggcgtc 1440atctcagaaa ccttcttgac gcccatcaat
ggcttcggcc tccaagctga tgagaacagc 1500aggctcatca ccttgacctg
caagagctac ctcagggagc tgctgctggc caccgacctc 1560agcaacaagg
agacaaagct catcgtgccg ccatcaggct tcatcagcaa catcgtggag
1620aatggcaacc tggaaggaga gaacctggag ccatggatag ccaacaacaa
gaatgcttat 1680gttgatcaca ccggcggcgt caatggaaca agggcgctct
atgttcacaa ggatggaggc 1740ttcagccagt tcatcggcga caagctgaag
cccaagacag aatatgtcat ccagtacacc 1800gtcaagggca agccatcaat
ccacctcaag aatgagaaca ccggctacat ccactacgag 1860gacaccaaca
acaacctgga ggactaccag accatcacca agaggttcac caccggcacc
1920gacctcaagg gcgtctacct catcttgaag agccaaaatg gagatgaagc
atggggagac 1980aacttcacca tcctggagat ctcgccatca gagaagctgc
tctcgccgga gctcatcaat 2040gtcaacaact ggatcagaac tggaagcacc
cacatcagcg gcaacacctt gacgctctac 2100caaggaggag gaggcaacct
caagcagaac ctccagcttg acagcttctc cacctacagg 2160gtgaacttct
ccgtcaccgg cgacgccaat gtgaggatca gaaattcaag ggaggtgctc
2220ttcgagaaga gatacatgag cggcgccaag gatgtttctg agatcttcac
caccaagctg 2280ggcaaggaca acttctacat cgagctgagc caaggcaaca
acctctatgg agggccgctg 2340gtgaagttca atgatgtgag catcaagcat
caccaccatc atcactaa 238863789PRTBacillus thuringiensis 63Met Asn
Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1 5 10 15
Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp 20
25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asn Leu Thr
Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Glu Ile
Ser Gly Lys 50 55 60 Leu Asp Gly Val
Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn
Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95
Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100
105 110 Met Leu His Ile Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp
Val 115 120 125 Met Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr
Leu Ser Lys 130 135 140 Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile
Ile Asn Val Asn Val 145 150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu
Ile Thr Pro Ala Tyr Gln Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys
Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr 180 185 190 Thr Leu Lys Val
Lys Lys Asp Ser Ser Pro Ala Asp Ile Leu Asp Glu 195 200 205 Leu Thr
Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val 210 215 220
Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly 225
230 235 240 Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu
Leu Ile 245 250 255 Ala Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val
Gly Asn Val Tyr 260 265 270 Asn Phe Leu Ile Val Leu Thr Ala Leu Gln
Ala Lys Ala Phe Leu Thr 275 280 285 Leu Thr Thr Cys Arg Lys Leu Leu
Gly Leu Ala Asp Ile Asp Tyr Thr 290 295 300 Ser Ile Met Asn Glu His
Leu Asn Lys Glu Lys Glu Glu Phe Arg Val 305 310 315 320 Asn Ile Leu
Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala 325 330 335 Lys
Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys 340 345
350 Pro Gly His Ala Leu Val Gly Phe Glu Met Ser Asn Asp Ser Ile Thr
355 360 365 Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln
Val Asp 370 375 380 Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met
Asp Lys Leu Leu 385 390 395 400 Cys Pro Asp Gln Ser Glu Gln Ile Tyr
Tyr Thr Asn Asn Ile Val Phe 405 410 415 Pro Asn Glu Tyr Val Ile Thr
Lys Ile Asp Phe Thr Lys Lys Met Lys 420 425 430 Thr Leu Arg Tyr Glu
Val Thr Ala Asn Ser Tyr Asp Ser Ser Thr Gly 435 440 445 Glu Ile Asp
Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr 450 455 460 Arg
Thr Leu Ser Ala Lys Asp Asp Gly Val Tyr Met Pro Leu Gly Val 465 470
475 480 Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln
Ala 485 490 495 Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser
Tyr Leu Arg 500 505 510 Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys
Glu Thr Lys Leu Ile 515 520 525 Val Pro Pro Ser Gly Phe Ile Ser Asn
Ile Val Glu Asn Gly Asn Leu 530 535 540 Glu Gly Glu Asn Leu Glu Pro
Trp Ile Ala Asn Asn Lys Asn Ala Tyr 545 550 555 560 Val Asp His Thr
Gly Gly Val Asn Gly Thr Arg Ala Leu Tyr Val His 565 570 575 Lys Asp
Gly Gly Phe Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys 580 585 590
Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His 595
600 605 Leu Lys Asn Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn
Asn 610 615 620 Asn Leu Glu Asp Tyr Gln Thr Ile Thr Lys Arg Phe Thr
Thr Gly Thr 625 630 635 640 Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys
Ser Gln Asn Gly Asp Glu 645 650 655 Ala Trp Gly Asp Asn Phe Thr Ile
Leu Glu Ile Ser Pro Ser Glu Lys 660 665 670 Leu Leu Ser Pro Glu Leu
Ile Asn Val Asn Asn Trp Ile Arg Thr Gly 675 680 685 Ser Thr His Ile
Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Gly 690 695 700 Gly Asn
Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg 705 710 715
720 Val Asn Phe Ser Val Thr Gly Asp Ala Asn Val Arg Ile Arg Asn Ser
725 730 735 Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys
Asp Val 740 745 750 Ser Glu Ile Phe Thr Thr Lys Leu Gly Lys Asp Asn
Phe Tyr Ile Glu 755 760 765 Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly
Pro Leu Val Lys Phe Asn 770 775 780 Asp Val Ser Ile Lys 785
64795PRTArtificial SequenceAXMI-005 w/ c-terminal extension 64Met
Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe 1 5 10
15 Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp
20 25 30 Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asn Leu
Thr Leu 35 40 45 Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Glu
Ile Ser Gly Lys 50 55 60 Leu Asp Gly Val Asn Gly Ser Leu Asn Asp
Leu Ile Ala Gln Gly Asn 65 70 75 80 Leu Asn Thr Glu Leu Ser Lys Glu
Ile Leu Lys Ile Ala Asn Glu Gln 85 90 95 Asn Gln Val Leu Asn Asp
Val Asn Asn Lys Leu Asp Ala Ile Asn Thr 100 105 110 Met Leu His Ile
Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val 115 120 125 Met Lys
Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys 130 135 140
Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val 145
150 155 160 Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln
Arg Ile 165 170 175 Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe
Ala Thr Glu Thr 180 185 190 Thr Leu Lys Val Lys Lys Asp Ser Ser Pro
Ala Asp Ile Leu Asp Glu 195 200 205 Leu Thr Glu Leu Thr Glu Leu Ala
Lys Ser Val Thr Lys Asn Asp Val 210 215 220 Asp Gly Phe Glu Phe Tyr
Leu Asn Thr Phe His Asp Val Met Val Gly 225 230 235 240 Asn Asn Leu
Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile 245 250 255 Ala
Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr 260 265
270 Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Lys Ala Phe Leu Thr
275 280 285 Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp
Tyr Thr 290 295 300 Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu
Glu Phe Arg Val 305 310 315 320 Asn Ile Leu Pro Thr Leu Ser Asn Thr
Phe Ser Asn Pro Asn Tyr Ala 325 330 335 Lys Val Lys Gly Ser Asp Glu
Asp Ala Lys Met Ile Val Glu Ala Lys 340 345 350 Pro Gly His Ala Leu
Val Gly Phe Glu Met Ser Asn Asp Ser Ile Thr 355 360 365 Val Leu Lys
Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp 370 375 380 Lys
Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu 385 390
395 400 Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile Val
Phe 405 410 415 Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys
Lys Met Lys 420 425 430 Thr Leu Arg Tyr Glu Val Thr Ala Asn Ser Tyr
Asp Ser Ser Thr Gly 435 440 445 Glu Ile Asp Leu Asn Lys Lys Lys Val
Glu Ser Ser Glu Ala Glu Tyr 450 455 460 Arg Thr Leu Ser Ala Lys Asp
Asp Gly Val Tyr Met Pro Leu Gly Val 465 470 475 480 Ile Ser Glu Thr
Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala 485 490 495 Asp Glu
Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg 500 505 510
Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile 515
520 525 Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Asn
Leu 530 535 540 Glu Gly Glu Asn Leu Glu Pro Trp Ile Ala Asn Asn Lys
Asn Ala Tyr 545 550 555 560 Val Asp His Thr Gly Gly Val Asn Gly Thr
Arg Ala Leu Tyr Val His 565 570 575 Lys Asp Gly Gly Phe Ser Gln Phe
Ile Gly Asp Lys Leu Lys Pro Lys 580 585 590 Thr Glu Tyr Val Ile Gln
Tyr Thr Val Lys Gly Lys Pro Ser Ile His 595 600 605 Leu Lys Asn Glu
Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn 610 615 620 Asn Leu
Glu Asp Tyr Gln Thr Ile Thr Lys Arg Phe Thr Thr Gly Thr 625 630 635
640 Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu
645 650 655 Ala Trp Gly Asp Asn Phe Thr Ile Leu Glu Ile Ser Pro Ser
Glu Lys 660 665 670 Leu Leu Ser Pro Glu Leu Ile Asn Val Asn Asn Trp
Ile Arg Thr Gly 675 680 685 Ser Thr His Ile Ser Gly Asn Thr Leu Thr
Leu Tyr Gln Gly Gly Gly 690 695 700 Gly Asn Leu Lys Gln Asn Leu Gln
Leu Asp Ser Phe Ser Thr Tyr Arg 705 710 715 720 Val Asn Phe Ser Val
Thr Gly Asp Ala Asn Val Arg Ile Arg Asn Ser 725 730 735 Arg Glu Val
Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val 740 745 750 Ser
Glu Ile Phe Thr Thr Lys Leu Gly Lys Asp Asn Phe Tyr Ile Glu 755 760
765 Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Leu Val Lys Phe Asn
770 775 780 Asp Val Ser Ile Lys His His His His His His 785 790 795
653537DNAArtificial Sequencesynthetic nucleotide sequence encoding
Cry1Ac (synFLCry1Ac) 65atggataaca accccaacat caacgagtgc atcccctaca
actgcctctc aaaccccgag 60gtcgaggtcc tcggcggtga gcgtatcgag accggttaca
cccctatcga tatcagcctg 120agcctgaccc agttcctcct ctcggagttc
gtccccggtg ctggtttcgt tctgggtttg 180gtcgatatca tctggggcat
cttcggcccc tcgcagtggg atgctttcct ggtccagatc 240gagcagttga
tcaaccagcg catcgaggag ttcgccagga accaggctat ctcacgtctg
300gagggtttgt cgaacctcta ccaaatctac gccgagagct tccgcgagtg
ggaggctgat 360cctactaacc ccgctttgcg tgaggagatg cgcatccagt
tcaacgatat gaactcggcc 420ctgaccaccg ccatccccct cttcgctgtc
cagaactacc aggtcccctt gctctcagtc 480tacgtccagg ctgctaacct
ccatctgagc gtcctgaggg atgtctctgt cttcggccag 540aggtggggtt
tcgatgctgc tactatcaac agccgctaca acgatctcac ccgcctgatc
600ggcaactaca ccgattacgc cgtccgttgg tacaacaccg gcctggagcg
tgtttggggt 660cctgattcac gtgattgggt ccgttacaac cagttccgca
gggagctcac cttgaccgtc 720ttggatatcg tcgccctctt ccccaactac
gatagccgcc gttaccccat ccgtaccgtt 780agccagctca ctagggaaat
ctacaccaac cccgtcctgg agaacttcga tggctcgttc 840cgtggttcag
ctcagggtat cgagaggagc atccgttcgc ctcatctgat ggatatcctg
900aactcgatca ccatctacac cgatgcccat cgcggctact actactggtc
gggccatcag 960atcatggcct cgcccgtcgg cttctcaggt cctgagttca
ccttcccctt gtacggcacc 1020atgggtaacg ccgctcctca gcagaggatc
gttgctcagt tgggtcaggg tgtttacagg 1080accctgagct ctaccctcta
caggaggcct ttcaacatcg gcatcaacaa ccagcagctg 1140tcagtcctcg
atggcactga gttcgcctac ggcaccagct ctaacctgcc ctctgctgtc
1200taccgcaaga gcggcaccgt tgattcgctc gatgagatcc cccctcagaa
caacaacgtc 1260ccccctcgtc agggtttctc acatcgtctg agccatgtct
cgatgttccg cagcggtttc 1320agcaactctt cggtctcgat catccgcgcc
cctatgttct cttggattca tcgctcggcc 1380gagttcaaca acatcatcgc
ctctgatagc atcactcaga tccccgccgt caagggcaac 1440ttcctgttca
acggctcagt gatcagcggt cctggcttca ctggtggcga tttggtccgc
1500ctgaactcgt cgggcaacaa catccagaac cgtggttaca tcgaggtccc
catccatttc 1560cctagcacct cgacccgtta ccgcgtgagg gtcaggtacg
cttcggttac ccctatccat 1620ttgaacgtta actggggcaa ctcatcgatc
ttctctaaca ccgtccccgc caccgccacc 1680tcgctggata acctccagag
ctctgatttc ggctacttcg agagcgccaa cgccttcacc 1740tccagcctgg
gcaacatcgt tggcgtgagg aacttctcag gcaccgctgg tgtcatcatc
1800gaccgcttcg agttcatccc cgtcaccgct accctggagg ctgagtacaa
cctggagagg 1860gctcagaagg ctgtgaacgc tctcttcacc agcactaacc
agctcggtct taagaccaac 1920gttaccgatt accatatcga ccaggtttca
aacctcgtca cctacctcag cgatgagttc 1980tgcctggatg agaagcgcga
gctgagcgag aaggtcaagc atgccaagcg cctgtcggat 2040gagcgcaacc
tgctccagga tagcaacttc aaggatatca accgtcagcc cgagaggggc
2100tggggcggtt caaccggtat caccatccag ggtggcgatg atgtcttcaa
ggagaactac 2160gtcaccctga gcggcacctt cgatgagtgc taccctacct
acttgtacca gaagatcgac 2220gagtctaagc tcaaggcctt cactcgctac
cagttgcgcg gttacatcga ggattcacag 2280gatctggaaa tctacctgat
ccgctacaac gctaagcatg agactgttaa cgtccctggc 2340accggttcgc
tctggcctct cagcgctcag tctcccatcg gcaagtgcgg cgagcctaac
2400cgttgcgccc ctcatttgga gtggaaccct gatctggatt gctcatgccg
cgatggcgag 2460aagtgcgccc atcatagcca tcatttctca ttggatatcg
atgtcggttg caccgatctg 2520aacgaggatc tcggtgtctg ggtcatcttc
aagatcaaga cccaggatgg tcatgcccgc 2580ctcggcaact tggagttcct
ggaggagaag cctctggtcg gcgaggcctt ggctagggtg 2640aagcgcgccg
agaagaagtg gcgcgataag cgcgagaagc tggagtggga gaccaacatc
2700gtctacaagg aggccaagga gtcagtcgat gccctgttcg tgaacagcca
gtacgatcag 2760ctccaggccg ataccaacat cgccatgatc catgccgccg
ataagcgcgt tcatagcatc 2820cgcgaggcct acctccctga gctctcagtg
atccctggtg ttaacgccgc tatcttcgag 2880gagctggagg gccgcatctt
cactgccttc agcctgtacg atgctcgtaa cgtgatcaag 2940aacggtgatt
tcaacaacgg tttgtcatgc tggaacgtga agggccatgt cgatgtcgag
3000gagcagaaca accagcgcag cgttctggtt gtccctgagt gggaggctga
ggtttcacag 3060gaggtccgcg tctgccctgg ccgtggttac atcctgaggg
tcaccgctta caaggagggt 3120tacggcgagg gttgcgttac tatccatgag
atcgagaaca acaccgatga gctcaagttc 3180tcaaactgcg tcgaggagga
aatctacccc aacaacactg tgacctgcaa cgattacact 3240gttaaccagg
aggagtacgg cggcgcctac accagccgta accgtggcta caacgaggct
3300ccctctgtcc ccgccgatta cgcctcagtc tacgaggaga agtcgtacac
cgatggccgc 3360cgcgagaacc cctgcgagtt caacaggggt taccgcgatt
acaccccctt gcccgtcggc 3420tacgtgacta aggagctgga gtacttcccc
gagactgata aggtctggat cgagatcggc 3480gagactgagg gcaccttcat
cgttgattca gtcgagctgc tgctcatgga ggagtga 3537663537DNAArtificial
Sequencesynthetic nucleotide sequence encoding Cry1Ac (NalCry1Ac)
66atggataaca accccaacat caacgagtgc atcccctaca actgcctctc aaaccccgag
60gtcgaggtcc tcggcggtga gcgtatcgag accggttaca cccctatcga tatcagcctg
120agcctgaccc agttcctcct ctcggagttc gtccccggtg ctggtttcgt
tctgggtttg 180gtcgatatca tctggggcat cttcggcccc tcgcagtggg
atgctttcct ggtccagatc 240gagcagttga tcaaccagcg catcgaggag
ttcgccagga accaggctat ctcacgtctg 300gagggtttgt cgaacctcta
ccaaatctac gccgagagct tccgcgagtg ggaggctgat 360cctactaacc
ccgctttgcg tgaggagatg cgcatccagt tcaacgatat gaactcggcc
420ctgaccaccg ccatccccct cttcgctgtc cagaactacc aggtcccctt
gctctcagtc 480tacgtccagg ctgctaacct ccatctgagc gtcctgaggg
atgtctctgt cttcggccag 540aggtggggtt tcgatgctgc tactatcaac
agccgctaca acgatctcac ccgcctgatc 600ggcaactaca ccgattacgc
cgtccgttgg tacaacaccg gcctggagcg tgtttggggt 660cctgattcac
gtgattgggt ccgttacaac cagttccgca gggagctcac cttgaccgtc
720ttggatatcg tcgccctctt ccccaactac gatagccgcc gttaccccat
ccgtaccgtt 780agccagctca ctagggaaat ctacaccaac cccgtcctgg
agaacttcga tggctcgttc 840cgtggttcag ctcagggtat cgagaggagc
atccgttcgc ctcatctgat ggatatcctg 900aactcgatca ccatctacac
cgatgcccat cgcggctact actactggtc gggccatcag 960atcatggcct
cgcccgtcgg cttctcaggt cctgagttca ccttcccctt gtacggcacc
1020atgggtaacg ccgctcctca gcagaggatc gttgctcagt tgggtcaggg
tgtttacagg 1080accctgagct ctaccctcta caggaggcct ttcaacatcg
gcatcaacaa ccagcagctg 1140tcagtcctcg atggcactga gttcgcctac
ggcaccagct ctaacctgcc ctctgctgtc 1200taccgcaaga gcggcaccgt
tgattcgctc gatgagatcc cccctcagaa caacaacgtc 1260ccccctcgtc
agggtttctc acatcgtctg agccatgtct cgatgttccg cagcggtttc
1320agcaactctt cggtctcgat catccgcgcc cctatgttct cttggattca
tcgctcggcc
1380gagttcaaca acatcatcgc ctctgatagc atcactcaga tccccgccgt
caagggcaac 1440ttcctgttca acggctcagt gatcagcggt cctggcttca
ctggtggcga tttggtccgc 1500ctgaactcgt cgggcaacaa catccagaac
cgtggttaca tcgaggtccc catccatttc 1560cctagcacct cgacccgtta
ccgcgtgagg gtcaggtacg cttcggttac ccctatccat 1620ttgaacgtta
actggggcaa ctcatcgatc ttctctaaca ccgtccccgc caccgccacc
1680tcgctggata acctccagag ctctgatttc ggctacttcg agagcgccaa
cgccttcacc 1740tccagcctgg gcaacatcgt tggcgtgagg aacttctcag
gcaccgctgg tgtcatcatc 1800gaccgcttcg agttcatccc cgtcaccgct
accctggagg ctgagtacaa cctggagagg 1860gctcagaagg ctgtgaacgc
tctcttcacc agcactaacc agctcggtct caagaccaac 1920gttaccgatt
accatatcga ccaggtttca aacctcgtca cctacctcag cgatgagttc
1980tgcctggatg agaagcgcga gctgagcgag aaggtcaagc atgccaagcg
cctgtcggat 2040gagcgcaacc tgctccagga tagcaacttc aaggatatca
accgtcagcc cgagaggggc 2100tggggcggtt caaccggtat caccatccag
ggtggcgatg atgtcttcaa ggagaactac 2160gtcaccctga gcggcacctt
cgatgagtgc taccctacct acttgtacca gaagatcgac 2220gagtctaagc
tcaaggcctt cactcgctac cagttgcgcg gttacatcga ggattcacag
2280gatctggaaa tctacctgat ccgctacaac gctaagcatg agactgttaa
cgtccctggc 2340accggttcgc tctggcctct cagcgctcag tctcccatcg
gcaagtgcgg cgagcctaac 2400cgttgcgccc ctcatttgga gtggaaccct
gatctggatt gctcatgccg cgatggcgag 2460aagtgcgccc atcatagcca
tcatttctca ttggatatcg atgtcggttg caccgatctg 2520aacgaggatc
tcggtgtctg ggtcatcttc aagatcaaga cccaggatgg tcatgcccgc
2580ctcggcaact tggagttcct ggaggagaag cctctggtcg gcgaggcctt
ggctagggtg 2640aagcgcgccg agaagaagtg gcgcgataag cgcgagaagc
tggagtggga gaccaacatc 2700gtctacaagg aggccaagga gtcagtcgat
gccctgttcg tgaacagcca gtacgatcag 2760ctccaggccg ataccaacat
cgccatgatc catgccgccg ataagcgcgt tcatagcatc 2820cgcgaggcct
acctccctga gctctcagtg atccctggtg ttaacgccgc tatcttcgag
2880gagctggagg gccgcatctt cactgccttc agcctgtacg atgctcgtaa
cgtgatcaag 2940aacggtgatt tcaacaacgg tttgtcatgc tggaacgtga
agggccatgt cgatgtcgag 3000gagcagaaca accagcgcag cgttctggtt
gtccctgagt gggaggctga ggtttcacag 3060gaggtccgcg tctgccctgg
ccgtggttac atcctgaggg tcaccgctta caaggagggt 3120tacggcgagg
gttgcgttac tatccatgag atcgagaaca acaccgatga gctcaagttc
3180tcaaactgcg tcgaggagga aatctacccc aacaacactg tgacctgcaa
cgattacact 3240gttaaccagg aggagtacgg cggcgcctac accagccgta
accgtggcta caacgaggct 3300ccctctgtcc ccgccgatta cgcctcagtc
tacgaggaga agtcgtacac cgatggccgc 3360cgcgagaacc cctgcgagtt
caacaggggt taccgcgatt acaccccctt gcccgtcggc 3420tacgtgacta
aggagctgga gtacttcccc gagactgata aggtctggat cgagatcggc
3480gagactgagg gcaccttcat cgttgattca gtcgagctgc tgctcatgga ggagtga
3537673537DNAArtificial Sequencesynthetic nucleotide sequence
encoding Cry1Ac (synCry1AcB) 67atggacaaca accccaacat caatgaatgc
atcccctaca actgcttgag caacccggag 60gtggaggtgc tgggaggaga aagaattgaa
actggctaca cgcccatcga catcagcttg 120agcttgacac aatttcttct
ttcagaattt gttcctggcg ccggcttcgt gctggggctg 180gtggacatca
tctggggcat cttcggccca agccaatggg atgccttcct ggtgcaaatt
240gagcagctca tcaaccagag gattgaagaa tttgcaagaa atcaagccat
ctcaaggctg 300gaagggctga gcaacctcta ccagatctac gccgagagct
tcagagaatg ggaagctgat 360ccaacaaatc ctgctcttcg agaagagatg
aggattcagt tcaacgacat gaactcggcg 420ctcaccaccg ccatcccgct
cttcgccgtc cagaactacc aagttcctct tctttcagtt 480tatgttcaag
ctgccaacct ccacctctcc gtgctgagag atgtttctgt ttttggacaa
540agatggggct tcgacgccgc caccatcaac agcagataca atgatttgac
aaggctcatc 600ggcaactaca ccgactacgc cgtccgctgg tacaacaccg
gcctggagag ggtgtgggga 660ccagattcaa gagattgggt gagatacaac
cagttcagaa gagagctcac cttgacggtg 720ctggacatcg tggcgctctt
ccccaactat gattcaagaa gatatcccat caggacggtg 780agccagctga
caagggagat ctacaccaac ccggtgctgg agaactttga tggcagcttc
840cgcggcagcg ctcaaggaat tgaaagaagc atcagaagcc ctcatctgat
ggacatcctc 900aacagcatca ccatctacac tgatgctcac cgcggctact
actactggag cggccaccag 960atcatggcat cacctgttgg cttctctgga
cctgagttca ccttcccgct ctatggaaca 1020atgggcaatg ctgctcctca
acaaagaatt gtggcgcagc tgggccaagg agtctacagg 1080acgctgagca
gcaccttgta ccggcggccc ttcaacatcg gcatcaacaa ccagcagctc
1140tccgtgctgg atggaactga atttgcttat ggaacatcaa gcaacctgcc
aagcgccgtc 1200tacaggaaga gcggcaccgt ggacagcttg gatgagatcc
cgccgcagaa caacaatgtg 1260ccgccgcgcc aaggcttcag ccaccgcctc
agccatgtga gcatgttcag aagcggcttc 1320agcaacagct ccgtcagcat
catcagggcg ccgatgttca gctggattca tcgctcagca 1380gagttcaaca
acatcattgc ttctgacagc atcacccaga tcccggcggt gaagggcaac
1440ttcctcttca atggaagcgt catctctgga cctggcttca ctggaggaga
tctggtgagg 1500ctcaacagca gcggcaacaa catccagaac agaggctaca
ttgaggtgcc catccacttc 1560ccatcaacat caacaagata cagggtgagg
gtgagatatg cttctgtgac gcccatccac 1620ctcaatgtca actggggcaa
cagcagcatc ttcagcaaca ccgtgccggc gacggcgacg 1680agcttggaca
accttcaaag ctcagatttt ggatattttg agagcgccaa cgccttcacc
1740tcctcgctgg gcaacattgt tggtgtgagg aacttcagcg gcaccgccgg
cgtcatcatc 1800gacagatttg agttcatccc ggtgacagca acattggagg
cggagtacaa cctagaaaga 1860gctcagaagg ccgtcaacgc gctcttcacc
tccaccaacc agctgggcct caagacaaat 1920gtcaccgact accacattga
tcaagtgagc aacctggtga cctacctctc tgatgagttc 1980tgcttggatg
agaagaggga gctctccgag aaggtgaagc atgccaagag gctctctgat
2040gaaaggaacc tgctgcaaga ttcaaacttc aaggacatca acaggcagcc
agaaagagga 2100tggggaggaa gcaccggcat caccatccaa ggaggagatg
atgtcttcaa ggagaactac 2160gtcaccttga gcggcacctt tgatgaatgc
taccccacct acctctacca gaagattgat 2220gaaagcaagc taaaggcctt
cacaagatac cagctccgcg gctacattga agattctcaa 2280gatctggaga
tctacctcat cagatacaac gccaagcatg agacggtgaa tgttcctgga
2340actggaagcc tctggccgct ctcagctcaa agccccatcg gcaaatgtgg
agagcccaac 2400cgctgcgcgc cgcacctgga gtggaatcca gatctggatt
gcagctgccg agatggagaa 2460aaatgtgctc atcacagcca ccacttctca
ttggacattg atgttggctg caccgacctc 2520aatgaagatc ttggagtttg
ggtgatcttc aagatcaaga ctcaagatgg ccatgcaagg 2580ctgggcaacc
tggagttcct ggaggagaag ccgctggtgg gagaagctct agcaagggtg
2640aagagagctg agaagaagtg gagggacaag agggagaagc tggagtggga
gaccaacatc 2700gtctacaagg aggccaagga gagcgtggat gctctcttcg
tcaacagcca atatgatcaa 2760cttcaagctg acaccaacat cgccatgatc
cacgccgccg acaagagggt gcacagcatc 2820agagaagcat atcttccaga
gctctcagtg atccccggcg tcaacgccgc catcttcgag 2880gagctggaag
gaaggatctt caccgccttc agcctctatg atgcaagaaa tgtcatcaag
2940aatggagact tcaacaatgg gctgagctgc tggaatgtga agggccatgt
tgatgtggag 3000gagcagaaca accaaagatc agtgctggtg gtgccagaat
gggaagcaga agtttctcaa 3060gaagttcgag tttgccccgg ccgcggctac
atcctccgcg tcaccgccta caaggaagga 3120tatggagaag gctgcgtcac
catccatgag attgagaaca acaccgacga gctgaagttc 3180tcaaattgtg
tggaggagga gatctacccc aacaacaccg tcacctgcaa tgactacacc
3240gtgaaccaag aagaatatgg cggcgcctac acctcaagga acagaggcta
caatgaagct 3300ccttctgttc ctgctgatta tgcttctgtc tacgaggaga
agagctacac tgatggaaga 3360agagaaaatc catgtgagtt caacagaggc
tacagggact acacgccgct acctgttgga 3420tatgtgacca aggagctgga
gtacttccca gaaactgaca aggtgtggat tgagattgga 3480gaaacagaag
gaaccttcat cgtggacagc gtggagctgc tgctgatgga ggagtag
3537683537DNAArtificial Sequencesynthetic nucleotide sequence
encoding Cry1Ac (synCry1AcC) 68atggacaaca accccaacat caatgaatgc
atcccctaca actgcttgag caacccggag 60gtggaggtgc tgggaggaga aagaattgaa
actggctaca cgcccatcga catcagcttg 120agcttgacac aatttcttct
ttcagaattt gttcctggag ctggcttcgt gctgggcttg 180gtggacatca
tctggggcat ctttggacca agccaatggg atgccttctt ggtgcaaatt
240gagcagctca tcaaccaaag aattgaagaa tttgcaagaa atcaagccat
ctcaaggctg 300gaaggattga gcaacctcta ccagatctat gctgagagct
tcagagaatg ggaagctgat 360ccaacaaatc ctgctcttcg agaagaaatg
aggattcaat tcaatgacat gaactcggcg 420ctcaccaccg ccatcccgct
cttcgccgtc cagaactacc aagttcctct tctttcagtt 480tatgttcaag
ctgccaacct ccacctctcc gtgctgagag atgtttctgt ttttggacaa
540agatggggct tcgacgccgc caccatcaac tcaagataca atgatttgac
aaggctcatc 600ggcaactaca ccgactacgc cgtcagatgg tacaacaccg
gcctggagag agtttgggga 660ccagattcaa gagattgggt gagatacaac
cagttcagaa gagagctgac attgacagtg 720ctggacattg tggcgctctt
cccaaattat gattcaagaa gatatcccat caggacggtg 780agccagctga
caagagagat ctacaccaac ccggtgctgg agaattttga tggcagcttc
840cgcggctctg ctcaaggaat tgaaagaagc atcagatctc ctcatttgat
ggacatcctc 900aacagcatca ccatctacac tgatgctcat cgaggctact
actactggag cggccaccag 960atcatggcat cacctgttgg cttctctgga
cctgagttca ccttcccgct ctatggaaca 1020atgggaaatg ctgctcctca
acaaagaatt gtggcgcagc tgggccaagg agtctacagg 1080acattgagca
gcaccttgta ccggcggcca ttcaacatcg gcatcaacaa ccagcagctc
1140tccgtgctgg atggaactga atttgcttat ggaacatcaa gcaaccttcc
aagcgccgtc 1200tacaggaaga gcggcaccgt ggacagcttg gatgagatcc
cgccgcagaa caacaatgtg 1260ccgccgcgcc aaggcttcag ccaccgcctc
agccatgtga gcatgttcag aagtggcttc 1320agcaacagct ccgtcagcat
catcagggcg ccaatgttca gctggattca tcgctcagca 1380gagttcaaca
acatcattgc ttctgacagc atcacccaga tcccggcggt gaagggcaac
1440ttcctcttca atggaagcgt catctctgga cctggcttca ctggaggaga
tttggtgagg 1500ctcaacagca gcggcaacaa catccagaac agaggctaca
ttgaagttcc catccacttc 1560ccatcaacat caacaagata cagggtgagg
gtgagatatg cttctgtgac gcccatccac 1620ctcaatgtca actggggcaa
cagcagcatc ttcagcaaca ccgtgccggc gacggcgacg 1680agcttggaca
accttcaaag ctcagatttt ggatattttg aaagcgccaa cgccttcacc
1740agcagcttgg gcaacattgt tggtgtgagg aacttcagcg gcaccgccgg
cgtcatcatc 1800gacagatttg agttcatccc ggtgacagca acattggaag
ctgagtacaa cctagaaaga 1860gctcagaagg ccgtcaatgc tctcttcacc
tccaccaacc agctgggctt gaagacaaat 1920gtcaccgact accacattga
tcaagtttca aatttggtga cctacctctc tgatgagttc 1980tgcttggatg
agaagaggga gctctcagag aaggtgaagc atgccaagag gctttctgat
2040gaaagaaatt tgctgcaaga ttcaaacttc aaggacatca acaggcaacc
agaaagagga 2100tggggaggaa gcaccggcat caccatccaa ggaggagatg
atgtcttcaa ggagaactat 2160gtcaccttga gcggcacctt tgatgaatgc
tatccaacct acctctacca gaagattgat 2220gaaagcaagc taaaggcctt
cacaagatat cagctgagag gctacattga agattctcaa 2280gatttggaga
tctacctcat cagatacaat gccaagcatg aaacagtgaa tgttcctgga
2340actggaagcc tctggccgct ctcagctcaa agccccattg gaaaatgtgg
agaaccaaac 2400agatgcgcgc cgcacctgga atggaatcca gatcttgatt
gcagctgccg agatggagaa 2460aaatgtgctc atcacagcca tcacttctca
ttggacattg atgttggatg caccgacctc 2520aatgaagatc ttggagtttg
ggtgatcttc aagatcaaga ctcaagatgg acatgcaagg 2580ctgggcaacc
tggagttctt ggaggagaag ccgctggtgg gagaagctct agcaagggtg
2640aagagagctg agaagaaatg gagagacaag agggagaagc tggaatggga
gaccaacatc 2700gtctacaagg aggccaagga gagcgtggat gctctcttcg
tcaacagcca atatgatcaa 2760cttcaagctg acaccaacat cgccatgatc
cacgccgccg acaagagggt gcacagcatc 2820agagaagcat atcttccaga
gctctcagtg attcctggcg tcaacgccgc catcttcgag 2880gagctggaag
gaaggatctt caccgccttc agcctctatg atgcaagaaa tgtcatcaag
2940aatggagatt tcaacaatgg cttgagctgc tggaatgtga agggccatgt
tgatgtggag 3000gagcagaaca accaaagatc agtgctggtg gtgccagaat
gggaagcaga agtttctcaa 3060gaagttcgag tttgtcctgg aagaggctac
atcctccgcg tcaccgccta caaagaagga 3120tatggagaag gatgtgtcac
catccatgaa attgagaaca acactgatga gctgaagttc 3180tcaaattgtg
tggaggagga gatctacccc aacaacaccg tcacctgcaa tgactacacc
3240gtgaatcaag aagaatatgg cggcgcctac acctcaagaa acagaggcta
caatgaagct 3300ccttctgttc ctgctgatta tgcttctgtt tatgaggaga
agagctacac tgatggaaga 3360agagaaaatc catgtgagtt caacagaggc
tacagagact acacgccgct acctgttgga 3420tatgtgacaa aggagctgga
gtacttccca gaaactgaca aggtgtggat tgaaattgga 3480gaaacagaag
gaaccttcat cgtggattct gtggagctgc tgctgatgga agaataa
3537693537DNAArtificial Sequencesynthetic nucleotide sequence
encoding Cry1Ac (optCry1Acv02) 69atggacaaca accccaacat caatgaatgc
atcccctaca actgcttgag caacccagag 60gtggaggtgc tgggaggaga aaggattgaa
actggctaca cccccatcga catctccctc 120tccctcaccc agttcctcct
ctcagaattt gttcctggag ctggcttcgt gctggggctg 180gtggacatca
tctggggcat cttcggccct tctcaatggg atgccttcct cgtccagatc
240gagcagctga tcaaccagag gattgaagaa tttgcaagga accaggccat
ctcaaggctg 300gaaggcctct ccaacctcta ccagatttat gctgagagct
tcagagaatg ggaagcagat 360ccaacaaatc ctgctctgag ggaggagatg
aggattcagt tcaatgacat gaactcagct 420ctcaccaccg ccatccctct
cttcgccgtc cagaactacc aggtgccgct gctctccgtc 480tatgttcaag
ctgccaacct ccacctctcc gtgctgagag atgtttcagt ttttggccaa
540agatggggct ttgatgctgc caccatcaac agcagataca atgatctgac
aaggctcatc 600ggcaactaca cagattatgc tgtcagatgg tacaacaccg
gcctggagcg cgtctggggg 660ccagattcaa gagattgggt gagatacaac
cagttcagaa gggagctcac cttgacggtg 720ctggacatcg tcgccctctt
ccccaactat gattcaagaa gatatcccat caggaccgtc 780agccagctga
caagggagat ctacaccaac cccgtgctgg agaactttga tggcagcttc
840agaggatcag ctcaaggaat tgaaagaagc atcagatctc ctcatctgat
ggacatcctc 900aacagcatca ccatctacac agatgctcac cgcggctact
actactggag cggccaccag 960atcatggctt ctcctgttgg cttctcagga
cctgagttca ccttccctct ctatggcacc 1020atgggcaacg ccgcgccgca
gcagaggatc gtcgcccagc tgggccaagg agtctacagg 1080accttgagca
gcaccctcta caggaggccc ttcaacatcg gcatcaacaa ccagcagctc
1140tccgtgctgg atggaactga atttgcatat ggaacaagca gcaaccttcc
ttcagctgtc 1200tacaggaaga gcggcaccgt ggacagcttg gatgaaattc
ctcctcaaaa caacaatgtg 1260ccgccgcgcc aaggcttcag ccaccgcctc
agccatgtga gcatgttcag aagcggcttc 1320agcaacagca gcgtcagcat
catcagggcg ccgatgttca gctggattca ccgctcagca 1380gagttcaaca
acatcattgc ttctgacagc atcacccaga tccccgccgt caagggcaac
1440ttcctcttca atggcagcgt catctccggc cctggcttca ctggaggaga
tctggtgagg 1500ctcaacagca gcggcaacaa catccagaac agaggctaca
ttgaggtgcc catccacttc 1560ccctccacct caacaagata cagggtgagg
gtgagatatg cttctgtcac ccccatccac 1620ctcaatgtca actggggcaa
cagcagcatc ttcagcaaca ctgttccagc aacagcaaca 1680agcctggaca
accttcaaag ctcagatttt ggatattttg aatcagcaaa tgccttcacc
1740agcagcttgg gcaacattgt tggagtgagg aacttctccg gcaccgccgg
cgtcatcatc 1800gacagatttg agttcatccc cgtcaccgcc accttggaag
cagagtacaa cctggagaga 1860gctcagaagg ccgtcaatgc tctcttcacc
tcaacaaacc agctgggcct caagacaaat 1920gtcaccgact accacattga
tcaagtcagc aacctggtga catatctctc tgatgagttc 1980tgcttggatg
agaagaggga gctctcagag aaggtgaagc atgccaagag gctctctgat
2040gaaaggaacc tcctccagga cagcaacttc aaggacatca acaggcagcc
agaaagagga 2100tggggaggaa gcaccggcat caccatccaa ggaggagatg
atgttttcaa ggagaactat 2160gtcaccctct ccggcacctt tgatgaatgc
taccccacct acctctacca gaagattgat 2220gaatcaaagc tgaaggcctt
cacaagatat cagctccgcg gctacatcga ggacagccaa 2280gatctggaga
tctacctcat cagatacaat gccaagcatg aaactgtcaa tgttcctgga
2340actggaagcc tctggccgct gagcgctcaa agccccattg gaaaatgtgg
agaaccaaac 2400agatgtgctc ctcatctgga atggaatcca gatctggact
gctcatgccg agatggagaa 2460aaatgtgctc accacagcca ccacttctcc
ttggacattg atgttggctg caccgacctc 2520aatgaagatc ttggagtttg
ggtgatcttc aagatcaaga ctcaagatgg ccatgcaagg 2580ctgggcaacc
tggagttcct ggaggagaag ccgctggtgg gagaagctct agcaagggtg
2640aagagagctg agaagaaatg gagggacaag agggagaagc tggaatggga
aacaaacatc 2700gtctacaagg aggccaagga gagcgtggat gctctcttcg
tcaacagcca atatgatcag 2760ctgcaagctg acaccaacat tgccatgatc
catgctgctg acaagagggt gcattcaatc 2820agagaagcat atcttccaga
gctctccgtc atccccggcg tcaatgctgc catcttcgag 2880gagctggaag
gaaggatctt caccgccttc tccctctatg atgcaagaaa tgtcatcaag
2940aatggagatt tcaacaatgg cctgagctgc tggaatgtca agggccatgt
tgatgtggag 3000gagcagaaca accaaagatc agtgctggtg gtgccagaat
gggaagcaga agtttctcaa 3060gaagtccgcg tctgccctgg aagaggctac
atcctccgcg tcaccgccta caaggaagga 3120tatggagaag gctgcgtcac
catccatgag attgagaaca acacagatga gctgaagttc 3180tcaaactgcg
tggaggagga gatctacccc aacaacaccg tcacatgcaa tgactacacc
3240gtgaaccaag aagaatatgg cggcgcctac acctcaagga acagaggcta
caatgaagct 3300ccttctgttc ctgctgatta tgcttctgtt tatgaggaga
agagctacac agatggaaga 3360agggagaacc cctgcgagtt caacagaggc
tacagggact acacgccgct acctgttgga 3420tatgtgacca aggagctgga
gtacttccca gaaacagaca aggtctggat tgagattgga 3480gaaacagaag
gaaccttcat cgtggacagc gtggagctgc tgctgatgga ggagtaa
3537701178PRTBacillus thuringiensis 70Met Asp Asn Asn Pro Asn Ile
Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 Ser Asn Pro Glu Val
Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 Tyr Thr Pro
Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45 Glu
Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50 55
60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn
Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln
Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr
Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met Arg Ile Gln Phe Asn Asp
Met Asn Ser Ala Leu Thr Thr Ala 130 135 140 Ile Pro Leu Phe Ala Val
Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 Tyr Val Gln
Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 Val
Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185
190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val
195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp
Ser Arg 210 215 220 Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu
Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Val Ala Leu Phe Pro Asn
Tyr Asp Ser Arg Arg Tyr Pro 245 250 255 Ile Arg Thr Val Ser Gln Leu
Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265 270 Leu Glu Asn Phe Asp
Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 Arg Ser Ile
Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln 305
310 315 320 Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr
Phe Pro 325 330 335 Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln
Arg Ile Val Ala 340 345 350 Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu
Ser Ser Thr Leu Tyr Arg 355 360 365 Arg Pro Phe Asn Ile Gly Ile Asn
Asn Gln Gln Leu Ser Val Leu Asp 370 375 380 Gly Thr Glu Phe Ala Tyr
Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400 Tyr Arg Lys
Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 Asn
Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425
430 Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445 Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe
Asn Asn 450 455 460 Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Ala
Val Lys Gly Asn 465 470 475 480 Phe Leu Phe Asn Gly Ser Val Ile Ser
Gly Pro Gly Phe Thr Gly Gly 485 490 495 Asp Leu Val Arg Leu Asn Ser
Ser Gly Asn Asn Ile Gln Asn Arg Gly 500 505 510 Tyr Ile Glu Val Pro
Ile His Phe Pro Ser Thr Ser Thr Arg Tyr Arg 515 520 525 Val Arg Val
Arg Tyr Ala Ser Val Thr Pro Ile His Leu Asn Val Asn 530 535 540 Trp
Gly Asn Ser Ser Ile Phe Ser Asn Thr Val Pro Ala Thr Ala Thr 545 550
555 560 Ser Leu Asp Asn Leu Gln Ser Ser Asp Phe Gly Tyr Phe Glu Ser
Ala 565 570 575 Asn Ala Phe Thr Ser Ser Leu Gly Asn Ile Val Gly Val
Arg Asn Phe 580 585 590 Ser Gly Thr Ala Gly Val Ile Ile Asp Arg Phe
Glu Phe Ile Pro Val 595 600 605 Thr Ala Thr Leu Glu Ala Glu Tyr Asn
Leu Glu Arg Ala Gln Lys Ala 610 615 620 Val Asn Ala Leu Phe Thr Ser
Thr Asn Gln Leu Gly Leu Lys Thr Asn 625 630 635 640 Val Thr Asp Tyr
His Ile Asp Gln Val Ser Asn Leu Val Thr Tyr Leu 645 650 655 Ser Asp
Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 660 665 670
Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser 675
680 685 Asn Phe Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly
Ser 690 695 700 Thr Gly Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys
Glu Asn Tyr 705 710 715 720 Val Thr Leu Ser Gly Thr Phe Asp Glu Cys
Tyr Pro Thr Tyr Leu Tyr 725 730 735 Gln Lys Ile Asp Glu Ser Lys Leu
Lys Ala Phe Thr Arg Tyr Gln Leu 740 745 750 Arg Gly Tyr Ile Glu Asp
Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 755 760 765 Tyr Asn Ala Lys
His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu 770 775 780 Trp Pro
Leu Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn 785 790 795
800 Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys
805 810 815 Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser
Leu Asp 820 825 830 Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu
Gly Val Trp Val 835 840 845 Ile Phe Lys Ile Lys Thr Gln Asp Gly His
Ala Arg Leu Gly Asn Leu 850 855 860 Glu Phe Leu Glu Glu Lys Pro Leu
Val Gly Glu Ala Leu Ala Arg Val 865 870 875 880 Lys Arg Ala Glu Lys
Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp 885 890 895 Glu Thr Asn
Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 900 905 910 Phe
Val Asn Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala 915 920
925 Met Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr
930 935 940 Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile
Phe Glu 945 950 955 960 Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser
Leu Tyr Asp Ala Arg 965 970 975 Asn Val Ile Lys Asn Gly Asp Phe Asn
Asn Gly Leu Ser Cys Trp Asn 980 985 990 Val Lys Gly His Val Asp Val
Glu Glu Gln Asn Asn Gln Arg Ser Val 995 1000 1005 Leu Val Val Pro
Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg 1010 1015 1020 Val Cys
Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys 1025 1030 1035
Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn 1040
1045 1050 Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu Glu
Ile 1055 1060 1065 Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr
Val Asn Gln 1070 1075 1080 Glu Glu Tyr Gly Gly Ala Tyr Thr Ser Arg
Asn Arg Gly Tyr Asn 1085 1090 1095 Glu Ala Pro Ser Val Pro Ala Asp
Tyr Ala Ser Val Tyr Glu Glu 1100 1105 1110 Lys Ser Tyr Thr Asp Gly
Arg Arg Glu Asn Pro Cys Glu Phe Asn 1115 1120 1125 Arg Gly Tyr Arg
Asp Tyr Thr Pro Leu Pro Val Gly Tyr Val Thr 1130 1135 1140 Lys Glu
Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile Glu 1145 1150 1155
Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu 1160
1165 1170 Leu Leu Met Glu Glu 1175 712219DNAArtificial
Sequenceaxmi-031(A-D) 71atggattgta atttacaatc acaacaaaat attccatata
atgtattagc aataccagta 60tctaatgtta attcgttgac tgatacagtt ggagatttaa
aaaaagcatg ggaagaattt 120caaaaaactg gttctttttc attaacagct
ttacaacaag gattttctgc ttcacaagga 180ggaacattca attatttaac
attactacaa tcaggaatat cattagctgg ttcttttgtt 240cctggaggta
cttttgtagc acctattatt aatatggtta ttggttggtt atggccacat
300aaaaacaaaa atgcggatac agaaaattta ataaatttaa ttgattcaga
aattcaaaaa 360caattaaaca aagctttatt agatgcagat agaaatgagt
ggagctctta tttagaatct 420atatttgatt cttcaaataa cctaaatggt
gcaattgtag atgcacagtg gtcaggcact 480gtaaatacta caaatagaac
actaagaaat ccaacagaat cagattatac aaatgttgtt 540acaaatttta
ttgcagcgga tggtgacatt gcaaataatg aaaatcacat aatgaatggc
600aactttgacg tagctgcagc accttatttt gttataggag caacagcacg
ttttgcagca 660atgcaatctt atattaaatt ttgtaatgct tggattgata
aagttggatt gagtgacgca 720cagcttacta cacaaaaggc taatttagat
cgcacgaaac aaaatatgcg taatgcaatt 780cttaactata cacaacaagt
tatgaaagtt tttaaagatt ccaaaaatat gcctacaata 840ggtactaata
aatttagtgt tgatacctat aatgtatata ttaaaggaat gacattaaat
900gttttagata ttgtagcaat atggccttca ttatatccag atgattatac
ttcacaaaca 960gccttagaac aaacacgtgt cactttttca aatatggttg
gccaagaaga aggtacagat 1020ggaagcctaa gaatttacaa tacttttgat
tcttttagtt atcaacatag tccaatacct 1080aataataatg ttaatttaat
ttcttattat aatgatgaat tacaaaatct agaattagga 1140gtatataccc
ctcctaaaaa aggaagtgga tactcttatc cttatggatt tgttttaaat
1200tatgcaaaca gtaaatataa atatggtgat agcaatgatc cagaatctct
aggaggatta 1260tctacactat ctgcacctat acaacaagtt aatgcagcaa
ctcaaaacag taaatatcta 1320gatggagaaa tcctaaatgg aataggagca
tccttacctg gttattgtac tacaggatgt 1380tcaccaacag aaccaccttt
tagttgtact tctaccgcta atggctataa agcaagctgt 1440aatccttcag
atacaaatca aaaaattaac gctttatatc cttttacaca agctaatgta
1500aagggaaaca caggaaaatt aggagtactg gcaagtcttg tttcatatga
tttaaatcct 1560aaaaatgtat ttggtgaatt agattcagat acaaataatg
ttatcttaaa aggaattcct 1620gcagaaaaag gatattttcc taataatgcg
cgtcctactg ttgtaaaaga atggattaat 1680ggtgcaagtg ctgtaccact
tgattcagga aataccttat ttatgacggc tacgaattta 1740acagctactc
aatatagaat tagaatacgt tatgcaaatc caaattcaaa tactcaaatc
1800ggtgtacgaa ttacacaaaa tggttctcta atttccagta gtaatctaac
actttatagt 1860actactgata tgaataatac tttaccacta aatgtatatg
taataggaga aaatggaaat 1920tatacacttc aagatttata taatactact
aatgttttat caacaggaga tattacatta 1980caaattacag gaggagatca
aaaaatattt attgatcgaa tagaatttgt tcctactatg 2040cctgtacctg
gtaatactaa caacaataac ggtaataata acggtaataa taatccccca
2100caccacgttt gtgcaatagc tggtacacaa caatcttgtt ctggaccgcc
caaatttgaa 2160caagtaagtg atttagaaaa aattacaaca caagtatata
tgttattcaa atcttatag 221972738PRTArtificial SequenceAXMI-031(A-D)
72Met Asp Cys Asn Leu Gln Ser Gln Gln Asn Ile Pro Tyr Asn Val Leu 1
5 10 15 Ala Ile Pro Val Ser Asn Val Asn Ser Leu Thr Asp Thr Val Gly
Asp 20 25 30 Leu Lys Lys Ala Trp Glu Glu Phe Gln Lys Thr Gly Ser
Phe Ser Leu 35 40 45 Thr Ala Leu Gln Gln Gly Phe Ser Ala Ser Gln
Gly Gly Thr Phe Asn 50 55 60 Tyr Leu Thr Leu Leu Gln Ser Gly Ile
Ser Leu Ala Gly Ser Phe Val 65 70 75 80 Pro Gly Gly Thr Phe Val Ala
Pro Ile Ile Asn Met Val Ile Gly Trp 85 90 95 Leu Trp Pro His Lys
Asn Lys Asn Ala Asp Thr Glu Asn Leu Ile Asn 100 105 110 Leu Ile Asp
Ser Glu Ile Gln Lys Gln Leu Asn Lys Ala Leu Leu Asp 115 120 125 Ala
Asp Arg Asn Glu Trp Ser Ser Tyr Leu Glu Ser Ile Phe Asp Ser 130 135
140 Ser Asn Asn Leu Asn Gly Ala Ile Val Asp Ala Gln Trp Ser Gly Thr
145 150 155 160 Val Asn Thr Thr Asn Arg Thr Leu Arg Asn Pro Thr Glu
Ser Asp Tyr 165 170 175 Thr Asn Val Val Thr Asn Phe Ile Ala Ala Asp
Gly Asp Ile Ala Asn 180 185 190 Asn Glu Asn His Ile Met Asn Gly Asn
Phe Asp Val Ala Ala Ala Pro 195 200 205 Tyr Phe Val Ile Gly Ala Thr
Ala Arg Phe Ala Ala Met Gln Ser Tyr 210 215 220 Ile Lys Phe Cys Asn
Ala Trp Ile Asp Lys Val Gly Leu Ser Asp Ala 225 230 235 240 Gln Leu
Thr Thr Gln Lys Ala Asn Leu Asp Arg Thr Lys Gln Asn Met 245 250 255
Arg Asn Ala Ile Leu Asn Tyr Thr Gln Gln Val Met Lys Val Phe Lys 260
265 270 Asp Ser Lys Asn Met Pro Thr Ile Gly Thr Asn Lys Phe Ser Val
Asp 275 280 285 Thr Tyr Asn Val Tyr Ile Lys Gly Met Thr Leu Asn Val
Leu Asp Ile 290 295 300 Val Ala Ile Trp Pro Ser Leu Tyr Pro Asp Asp
Tyr Thr Ser Gln Thr 305 310 315 320 Ala Leu Glu Gln Thr Arg Val Thr
Phe Ser Asn Met Val Gly Gln Glu 325 330 335 Glu Gly Thr Asp Gly Ser
Leu Arg Ile Tyr Asn Thr Phe Asp Ser Phe 340 345 350 Ser Tyr Gln His
Ser Pro Ile Pro Asn Asn Asn Val Asn Leu Ile Ser 355 360 365 Tyr Tyr
Asn Asp Glu Leu Gln Asn Leu Glu Leu Gly Val Tyr Thr Pro 370 375 380
Pro Lys Lys Gly Ser Gly Tyr Ser Tyr Pro Tyr Gly Phe Val Leu Asn 385
390 395 400 Tyr Ala Asn Ser Lys Tyr Lys Tyr Gly Asp Ser Asn Asp Pro
Glu Ser 405 410 415 Leu Gly Gly Leu Ser Thr Leu Ser Ala Pro Ile Gln
Gln Val Asn Ala 420 425 430 Ala Thr Gln Asn Ser Lys Tyr Leu Asp Gly
Glu Ile Leu Asn Gly Ile 435 440 445 Gly Ala Ser Leu Pro Gly Tyr Cys
Thr Thr Gly Cys Ser Pro Thr Glu 450 455 460 Pro Pro Phe Ser Cys Thr
Ser Thr Ala Asn Gly Tyr Lys Ala Ser Cys 465 470 475 480 Asn Pro Ser
Asp Thr Asn Gln Lys Ile Asn Ala Leu Tyr Pro Phe Thr 485 490 495 Gln
Ala Asn Val Lys Gly Asn Thr Gly Lys Leu Gly Val Leu Ala Ser 500 505
510 Leu Val Ser Tyr Asp Leu Asn Pro Lys Asn Val Phe Gly Glu Leu Asp
515 520 525 Ser Asp Thr Asn Asn Val Ile Leu Lys Gly Ile Pro Ala Glu
Lys Gly 530 535 540 Tyr Phe Pro Asn Asn Ala Arg Pro Thr Val Val Lys
Glu Trp Ile Asn 545 550 555 560 Gly Ala Ser Ala Val Pro Leu Asp Ser
Gly Asn Thr Leu Phe Met Thr 565 570 575 Ala Thr Asn Leu Thr Ala Thr
Gln Tyr Arg Ile Arg Ile Arg Tyr Ala 580 585 590 Asn Pro Asn Ser Asn
Thr Gln Ile Gly Val Arg Ile Thr Gln Asn Gly 595 600 605 Ser Leu Ile
Ser Ser Ser Asn Leu Thr Leu Tyr Ser Thr Thr Asp Met 610 615 620 Asn
Asn Thr Leu Pro Leu Asn Val Tyr Val Ile Gly Glu Asn Gly Asn 625 630
635 640 Tyr Thr Leu Gln Asp Leu Tyr Asn Thr Thr Asn Val Leu Ser Thr
Gly 645 650 655 Asp Ile Thr Leu Gln Ile Thr Gly Gly Asp Gln Lys Ile
Phe Ile Asp 660 665 670 Arg Ile Glu Phe Val Pro Thr Met Pro Val Pro
Gly Asn Thr Asn Asn 675 680 685 Asn Asn Gly Asn Asn Asn Gly Asn Asn
Asn Pro Pro His His Val Cys 690 695 700 Ala Ile Ala Gly Thr Gln Gln
Ser Cys Ser Gly Pro Pro Lys Phe Glu 705 710 715 720 Gln Val Ser Asp
Leu Glu Lys Ile Thr Thr Gln Val Tyr Met Leu Phe 725 730 735 Lys Ser
731083DNABacillus thuringiensisCDS(1)..(1083) 73atg aga att aga agg
aac caa tcc act ctg agc cat aat gaa cgc cta 48Met Arg Ile Arg Arg
Asn Gln Ser Thr Leu Ser His Asn Glu Arg Leu 1 5 10 15 gcg ttt act
aat gcg gta tta gaa tta aaa cgt aga cca agt cgt tta 96Ala Phe Thr
Asn Ala Val Leu Glu Leu Lys Arg Arg Pro Ser Arg Leu 20 25 30 ccg
atg tca ttg ggt agt aca agt cgt tat gat gat tat gtt tat tgg 144Pro
Met Ser Leu Gly Ser Thr Ser Arg Tyr Asp Asp Tyr Val Tyr Trp 35 40
45 cat tta cag tca atg gaa aat caa aca tcg act aca cca gga tgg gct
192His Leu Gln Ser Met Glu Asn Gln Thr Ser Thr Thr Pro Gly Trp Ala
50 55 60 cat aga ggc cca gca ttt tta cct tgg cat cgt tat tat cta
aat caa 240His Arg Gly Pro Ala Phe Leu Pro Trp His Arg Tyr Tyr Leu
Asn Gln 65 70 75 80 ttt gaa gaa gat tta caa cga att gat cat aca gtt
aca ctt cct tat 288Phe Glu Glu Asp Leu Gln Arg Ile Asp His Thr Val
Thr Leu Pro Tyr 85 90 95 tgg gat tgg aca gtt gat aac tca act gat
tca tca gtt cca gga agt 336Trp Asp Trp Thr Val Asp Asn Ser Thr Asp
Ser Ser Val Pro Gly Ser 100 105 110 cct tgg act gat gat ttt atg ggc
ggt gat ggt gat cct acc caa gaa 384Pro Trp Thr Asp Asp Phe Met Gly
Gly Asp Gly Asp Pro Thr Gln Glu 115 120 125 tat act gtc aca aca ggt
ccc ttt aca ggt gac aat tgg aag tta act 432Tyr Thr Val Thr Thr Gly
Pro Phe Thr Gly Asp Asn Trp Lys Leu Thr 130 135 140 ctt ttt gat cat
cat gaa aac gag cct cat aat gct cga tta cgc cgt 480Leu Phe Asp His
His Glu Asn Glu Pro His Asn Ala Arg Leu Arg Arg 145 150 155 160 cag
tta gga act act tta aat gcc tct gga aat act ata tca atc aat 528Gln
Leu Gly Thr Thr Leu Asn Ala Ser Gly Asn Thr Ile Ser Ile Asn 165 170
175 ctt cca aca gat tca gag gta cag aat tgt tta tta gaa act cca tat
576Leu Pro Thr Asp Ser Glu Val Gln Asn Cys Leu Leu Glu Thr Pro Tyr
180
185 190 tat gta tct cct tgg cgt gca ggg caa gat gta aat caa cct gca
tta 624Tyr Val Ser Pro Trp Arg Ala Gly Gln Asp Val Asn Gln Pro Ala
Leu 195 200 205 aat cca aca aaa cca agt ttt tgt aat cgt ctt gaa ggt
tgg tat gga 672Asn Pro Thr Lys Pro Ser Phe Cys Asn Arg Leu Glu Gly
Trp Tyr Gly 210 215 220 gca gga agt att cat aat aaa gtt cat gta tgg
gta gct ggt gct aca 720Ala Gly Ser Ile His Asn Lys Val His Val Trp
Val Ala Gly Ala Thr 225 230 235 240 gag ggc tct atg att tgg atg agc
tca cca aat gat cct gtc ttt ttc 768Glu Gly Ser Met Ile Trp Met Ser
Ser Pro Asn Asp Pro Val Phe Phe 245 250 255 tta cat cat gca aat att
gat cgc cta tgg gtc caa tgg cag gcc aat 816Leu His His Ala Asn Ile
Asp Arg Leu Trp Val Gln Trp Gln Ala Asn 260 265 270 aat cca aat gaa
ggg tat cat cct act gga aat ggt aat gaa gtt gga 864Asn Pro Asn Glu
Gly Tyr His Pro Thr Gly Asn Gly Asn Glu Val Gly 275 280 285 cca aca
ggt cat aat tta aat gat tca atg aat cct tgg ggg agg aag 912Pro Thr
Gly His Asn Leu Asn Asp Ser Met Asn Pro Trp Gly Arg Lys 290 295 300
gtt act cca aat aat gtc ctt aat cat tat agt ctt ggt tat act tac
960Val Thr Pro Asn Asn Val Leu Asn His Tyr Ser Leu Gly Tyr Thr Tyr
305 310 315 320 gat aca gat tca acc cct ctt tct gaa atc ttt atg cat
aca ttt aat 1008Asp Thr Asp Ser Thr Pro Leu Ser Glu Ile Phe Met His
Thr Phe Asn 325 330 335 ctg aaa att cgt aaa gaa aaa caa atc aaa gat
ggt cat ttt ggt tta 1056Leu Lys Ile Arg Lys Glu Lys Gln Ile Lys Asp
Gly His Phe Gly Leu 340 345 350 agt caa gaa gat tta gac aaa ttg taa
1083Ser Gln Glu Asp Leu Asp Lys Leu 355 360 74360PRTBacillus
thuringiensis 74Met Arg Ile Arg Arg Asn Gln Ser Thr Leu Ser His Asn
Glu Arg Leu 1 5 10 15 Ala Phe Thr Asn Ala Val Leu Glu Leu Lys Arg
Arg Pro Ser Arg Leu 20 25 30 Pro Met Ser Leu Gly Ser Thr Ser Arg
Tyr Asp Asp Tyr Val Tyr Trp 35 40 45 His Leu Gln Ser Met Glu Asn
Gln Thr Ser Thr Thr Pro Gly Trp Ala 50 55 60 His Arg Gly Pro Ala
Phe Leu Pro Trp His Arg Tyr Tyr Leu Asn Gln 65 70 75 80 Phe Glu Glu
Asp Leu Gln Arg Ile Asp His Thr Val Thr Leu Pro Tyr 85 90 95 Trp
Asp Trp Thr Val Asp Asn Ser Thr Asp Ser Ser Val Pro Gly Ser 100 105
110 Pro Trp Thr Asp Asp Phe Met Gly Gly Asp Gly Asp Pro Thr Gln Glu
115 120 125 Tyr Thr Val Thr Thr Gly Pro Phe Thr Gly Asp Asn Trp Lys
Leu Thr 130 135 140 Leu Phe Asp His His Glu Asn Glu Pro His Asn Ala
Arg Leu Arg Arg 145 150 155 160 Gln Leu Gly Thr Thr Leu Asn Ala Ser
Gly Asn Thr Ile Ser Ile Asn 165 170 175 Leu Pro Thr Asp Ser Glu Val
Gln Asn Cys Leu Leu Glu Thr Pro Tyr 180 185 190 Tyr Val Ser Pro Trp
Arg Ala Gly Gln Asp Val Asn Gln Pro Ala Leu 195 200 205 Asn Pro Thr
Lys Pro Ser Phe Cys Asn Arg Leu Glu Gly Trp Tyr Gly 210 215 220 Ala
Gly Ser Ile His Asn Lys Val His Val Trp Val Ala Gly Ala Thr 225 230
235 240 Glu Gly Ser Met Ile Trp Met Ser Ser Pro Asn Asp Pro Val Phe
Phe 245 250 255 Leu His His Ala Asn Ile Asp Arg Leu Trp Val Gln Trp
Gln Ala Asn 260 265 270 Asn Pro Asn Glu Gly Tyr His Pro Thr Gly Asn
Gly Asn Glu Val Gly 275 280 285 Pro Thr Gly His Asn Leu Asn Asp Ser
Met Asn Pro Trp Gly Arg Lys 290 295 300 Val Thr Pro Asn Asn Val Leu
Asn His Tyr Ser Leu Gly Tyr Thr Tyr 305 310 315 320 Asp Thr Asp Ser
Thr Pro Leu Ser Glu Ile Phe Met His Thr Phe Asn 325 330 335 Leu Lys
Ile Arg Lys Glu Lys Gln Ile Lys Asp Gly His Phe Gly Leu 340 345 350
Ser Gln Glu Asp Leu Asp Lys Leu 355 360 751083DNAArtificial
SequenceSynthetic sequence encoding AXN-2 75atgaggatca gaagaaacca
gtctaccttg tctcataacg agaggcttgc tttcactaac 60gctgtgcttg agcttaagag
aaggccatct aggcttccaa tgtctcttgg atctacctcc 120agatacgatg
attacgtgta ctggcacctt caatctatgg aaaaccagac ttctactact
180ccaggatggg ctcatagagg accagctttt ttgccatggc acaggtatta
tctcaaccag 240ttcgaagagg atcttcagag gattgatcat accgttaccc
ttccatattg ggattggacc 300gtggataact ctaccgattc ttctgttcca
ggatctccat ggactgatga tttcatggga 360ggtgatggtg atccaactca
agagtacact gttactactg gaccattcac tggtgataac 420tggaagctca
cccttttcga tcatcatgag aacgaaccac ataacgctag acttagaagg
480caacttggaa ctacccttaa cgcttccgga aacaccattt ccattaacct
tccaaccgat 540tctgaggttc agaactgcct tcttgagact ccttactacg
tttcaccttg gagagctgga 600caagatgtta accagccagc tcttaaccca
actaagccat ctttctgcaa cagacttgag 660ggatggtatg gtgctggatc
tattcataac aaagtgcatg tttgggtggc aggtgctact 720gaaggatcta
tgatctggat gtcctctcca aacgatccag ttttcttcct tcaccacgct
780aacattgata ggctttgggt tcaatggcaa gctaacaacc caaacgaggg
atatcatcca 840actggaaacg gaaacgaagt tggaccaacc ggacataacc
ttaacgattc catgaaccca 900tggggaagaa aggttacccc aaacaacgtt
cttaaccact actctctcgg atacacttac 960gatactgatt ctaccccact
ctccgagatt ttcatgcaca ccttcaacct caagatcagg 1020aaagagaagc
agattaagga cggacatttc ggactttctc aagaggatct cgacaagctc 1080tga
1083
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