U.S. patent application number 13/911549 was filed with the patent office on 2013-10-17 for beta-lactamase inhibitors.
The applicant listed for this patent is Merck Sharp & Dohme Corp.. Invention is credited to Ian MANGION, Nelo Rivera, Rebecca T. Ruck, Michael Shelvin.
Application Number | 20130274475 13/911549 |
Document ID | / |
Family ID | 40642204 |
Filed Date | 2013-10-17 |
United States Patent
Application |
20130274475 |
Kind Code |
A1 |
MANGION; Ian ; et
al. |
October 17, 2013 |
BETA-LACTAMASE INHIBITORS
Abstract
Substituted bicyclic beta-lactams of Formula I: ##STR00001## are
.beta.-lactamase inhibitors, wherein a, X, R.sup.1 and R.sup.2 are
defined herein. The compounds and pharmaceutically acceptable salts
thereof are useful in the treatment of bacterial infections in
combination with .beta.-lactam antibiotics. In particular, the
compounds can be employed with a .beta.-lactam antibiotics (e.g.,
imipenem, piperacillin, or ceftazidime) against microorganisms
resistant to .beta.-lactam antibiotics due to the presence of the
.beta.-lactamases.
Inventors: |
MANGION; Ian; (Cranford,
NJ) ; Rivera; Nelo; (New Milford, NJ) ; Ruck;
Rebecca T.; (Jersey City, NJ) ; Shelvin; Michael;
(Middlesex, NJ) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Merck Sharp & Dohme Corp. |
Rahway |
NJ |
US |
|
|
Family ID: |
40642204 |
Appl. No.: |
13/911549 |
Filed: |
June 6, 2013 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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12812763 |
Jul 14, 2010 |
8487093 |
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PCT/US2009/031047 |
Jan 15, 2009 |
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13911549 |
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61011533 |
Jan 18, 2008 |
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Current U.S.
Class: |
546/121 ;
546/188; 546/189; 546/244 |
Current CPC
Class: |
C07D 487/08 20130101;
A61P 31/00 20180101; A61P 9/00 20180101; C07D 211/60 20130101; C07D
471/08 20130101; A61P 43/00 20180101; C07D 211/58 20130101; A61P
31/04 20180101; C07D 519/00 20130101; C07D 211/78 20130101 |
Class at
Publication: |
546/121 ;
546/188; 546/189; 546/244 |
International
Class: |
C07D 471/08 20060101
C07D471/08; C07D 211/78 20060101 C07D211/78; C07D 211/60 20060101
C07D211/60; C07D 211/58 20060101 C07D211/58 |
Claims
1. A compound of Formula I: ##STR00279## or a pharmaceutically
acceptable salt thereof, wherein: the bond identified as "a" is a
single bond or a double bond; when bond a is a single bond, X is:
(1) CH.sub.2, (2) CH.sub.2CH.sub.2, (3) CH.sub.2CH.sub.2CH.sub.2,
(4) CH.dbd.CH, (5) CH.sub.2--CH.dbd.CH, or (6) CH.dbd.CH--CH.sub.2;
when bond a is a double bond, X is: (1) CH, (2) CH--CH.sub.2, or
(3) CH--CH.dbd.CH; R.sup.1 is: (1) C(O)N(R.sup.3)R.sup.4, (2)
C(O)OR.sup.3, or (3) C(O)OR.sup.5; R.sup.2 is SO.sub.3M,
OSO.sub.3M, SO.sub.2NH.sub.2, PO.sub.3M, OPO.sub.3M,
CH.sub.2CO.sub.2M, CF.sub.2CO.sub.2M, or CF.sub.3; M is H or a
pharmaceutically acceptable cation; R.sup.3 is: (1) C.sub.1-8 alkyl
substituted with a total of from 1 to 4 substituents selected from
the group consisting of (i) zero to 2 N(R.sup.A)R.sup.B, (ii) zero
to 2 R.sup.C, and (iii) zero to 1 of AryA, HetA, or HetB, (2) CycA,
(3) HetA, (4) AryA, (5) HetB, or (6) AryB; R.sup.4 is H or
C.sub.1-8 alkyl optionally substituted with N(R.sup.A)R.sup.B; or
alternatively, when R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 and
R.sup.4 together with the N atom to which they are both attached
form a 4- to 9-membered, saturated monocyclic ring optionally
containing 1 heteroatom in addition to the nitrogen attached to
R.sup.3 and R.sup.4 selected from N, O, and S, where the S is
optionally oxidized to S(O) or S(O).sub.2; wherein the monocyclic
ring is optionally fused to, bridged with, or spiro to a 4- to
7-membered, saturated heterocyclic ring containing from 1 to 3
heteroatoms independently selected from N, O and S, where the S is
optionally oxidized to S(O) or S(O).sub.2, to form a bicyclic ring
system, wherein the monocyclic ring or the bicyclic ring system so
formed is optionally substituted with 1 or 2 substituents each of
which is independently: (1) C.sub.1-6 alkyl, (2) C.sub.1-6
fluoroalkyl, (3) (CH.sub.2).sub.1-2G wherein G is OH, O--C.sub.1-6
alkyl, O--C.sub.1-6 fluoroalkyl, N(R.sup.A)R.sup.B,
C(O)N(R.sup.A)R.sup.B, C(O)R.sup.A, CO.sub.2R.sup.A, or
SO.sub.2R.sup.A, (4) O--C.sub.1-6 alkyl, (5) O--C.sub.1-6
fluoroalkyl, (6) OH, (7) oxo, (8) halogen, (9) N(R.sup.A)R.sup.B,
(10) C(O)N(R.sup.A)R.sup.B, (11) C(O)R.sup.A, (12) C(O)--C.sub.1-6
fluoroalkyl, (13) C(O)OR.sup.A, or (14) S(O).sub.2R.sup.A; R.sup.5
is C.sub.1-8 alkyl substituted with 1 or 2 substituents each of
which is independently N(R.sup.A)C(O)-AryA; CycA is C.sub.4-9
cycloalkyl which is optionally substituted with a total of from 1
to 4 substituents selected from zero to 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; HetA is a 4- to 9-membered saturated or
mono-unsaturated heterocyclic ring containing from 1 to 3
heteroatoms independently selected from N, O and S, wherein any
ring S is optionally oxidized to S(O) or S(O).sub.2 and either 1 or
2 ring carbons are optionally oxidized to C(O); wherein the ring is
optionally fused with a C.sub.3-7 cycloalkyl; and wherein the
optionally fused, saturated or mono-unsaturated heterocyclic ring
is optionally substituted with a total of from 1 to 4 substituents
selected from zero to 2 (CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero
to 2 (CH.sub.2).sub.nR.sup.C; AryA is phenyl which is optionally
substituted with a total of from 1 to 4 substituents selected from
zero to 2 (CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; HetB is a 5- or 6-membered heteroaromatic
ring containing from 1 to 4 heteroatoms selected from 1 to 3 N
atoms, zero or 1 O atom, and zero or 1 S atom; wherein the
heteroaromatic ring is optionally fused with a 5- to 7-membered,
saturated heterocyclic ring containing 1 or 2 heteroatoms
independently selected from N, O and S, wherein any ring S is
optionally oxidized to S(O) or S(O).sub.2 and either 1 or 2
non-fused ring carbons are optionally oxidized to C(O); and wherein
the optionally fused heteroaromatic ring is optionally substituted
with a total of from 1 to 4 substituents selected from zero to 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; AryB is a bicyclic ring system which is
phenyl fused with a 5- to 7-membered saturated heterocyclic ring
containing from 1 to 3 heteroatoms independently selected from N, O
and S, wherein any ring S is optionally oxidized to S(O) or
S(O).sub.2, and wherein the bicyclic ring system is optionally
substituted with a total of from 1 to 4 substituents selected from
zero to 2 (CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; each n is independently an integer which
is 0, 1, 2, or 3; each R.sup.A is independently H or C.sub.1-8
alkyl; each R.sup.B is independently H or C.sub.1-8 alkyl; each
R.sup.C is independently C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl,
OC(O)--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2,
halogen, CN, C(O)R.sup.A, C(O)OR.sup.A, C(O)N(R.sup.A)R.sup.B,
SO.sub.2R.sup.A, SO.sub.2N(R.sup.A)R.sup.B, pyridyl, pyrrolidinyl,
piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl; and
provided that: (A) when R.sup.1 is C(O)OR.sup.3 and R.sup.3 is
AryA, then AryA is not (i) unsubstituted phenyl, (ii) phenyl
substituted with NH.sub.2, (iii) phenyl substituted with OH, (iii)
phenyl substituted with O--C.sub.1-6 alkyl, (iv) phenyl substituted
with one or more halogens, or (v) phenyl substituted with C.sub.1-6
alkyl; (B) when R.sup.1 is C(O)OR.sup.3 and R.sup.3 is C.sub.1-6
alkyl substituted with HetB, then HetB is not pyridyl; (C) when
R.sup.1 is C(O)OR.sup.3 and R.sup.3 is CH.sub.2-AryA or
CH.sub.2CH.sub.2-AryA, then AryA is not (i) unsubstituted phenyl,
(ii) phenyl substituted with NH.sub.2, OH, O--C.sub.1-6 alkyl, or
C.sub.1-6 alkyl, or (iii) phenyl substituted with one or more
halogens; (D) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 is
AryA, CH.sub.2-AryA or CH.sub.2CH.sub.2-AryA, and R.sup.4 is H or
C.sub.1-6 alkyl, then AryA is not unsubstituted phenyl, phenyl
substituted with N(CH.sub.3).sub.2, or phenyl substituted with
C(O)NH.sub.2; (E) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 is
C.sub.1-6 alkyl substituted with HetB, and R.sup.4 is H or
C.sub.1-6 alkyl, then HetB is not pyridyl; and (F) when R.sup.1 is
C(O)OR.sup.3 and R.sup.3 is C.sub.1-6 alkyl substituted with
R.sup.C, then R.sup.C is not C(O)NH.sub.2.
2. The compound according to claim 1, or a pharmaceutically
acceptable salt thereof, wherein bond a is a single bond and X is
--CH.sub.2-- or --CH.sub.2CH.sub.2--.
3. The compound according to claim 1 or claim 2, or a
pharmaceutically acceptable salt thereof, wherein R.sup.2 is
OSO.sub.3M.
4. The compound according to claim 6, or a pharmaceutically
acceptable salt thereof, wherein R.sup.2 is OSO.sub.3H.
5. The compound according to claim 1, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is
C(O)N(R.sup.3)R.sup.4.
6. The compound according to claim 5, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetA, CH.sub.2-HetA,
CH.sub.2CH.sub.2-HetA, CH(CH.sub.3)-HetA, or
CH(CH.sub.2OH)-HetA.
7. The compound according to claim 6, or a pharmaceutically
acceptable salt thereof, wherein HetA is an optionally fused,
saturated heterocyclic ring selected from the group consisting of
azetidinyl, pyrrolidinyl, oxopyrrolidinyl, piperidinyl,
piperazinyl, tetrahydropyranyl, tetrahydrothiopyranyl, morpholinyl,
1,1-dioxidotetrahydrothiopyranyl, azepanyl, oxazepanyl, azocanyl,
and azabicyclo[3.1.0]cyclohexyl, wherein the heterocyclic is
optionally substituted with 1 or 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C.
8. The compound according to claim 6, or a pharmaceutically
acceptable salt thereof, which is a compound selected from the
group consisting of: ##STR00280## ##STR00281## ##STR00282## wherein
T is H, C.sub.1-3 alkyl, pyrrolidin-3-yl, piperidin-4-yl,
(CH.sub.2).sub.2-3--O--C.sub.1-3 alkyl, (CH.sub.2).sub.2-3OH,
(CH.sub.2).sub.2-3F, (CH.sub.2).sub.2-3-piperidinyl,
(CH.sub.2).sub.2-3-pyrrolidinyl; and T' is H, Cl, Br, F, C.sub.1-3
alkyl, O--C.sub.1-3 alkyl, OH, NH.sub.2, N(H)--C.sub.1-3 alkyl, or
N(--C.sub.1-3 alkyl).sub.2.
9. The compound according to claim 8, or a pharmaceutically
acceptable salt thereof, wherein T is H, CH.sub.3, pyrrolidin-3-yl,
piperidin-4-yl, (CH.sub.2).sub.2-3OCH.sub.3, (CH.sub.2).sub.2-3OH,
(CH.sub.2).sub.2-3F, (CH.sub.2).sub.2-3-piperidinyl,
(CH.sub.2).sub.2-3-pyrrolidinyl; and T' is H, F, O--C.sub.1-3
alkyl, OH, NH.sub.2, N(H)CH.sub.3, N(CH.sub.3).sub.2.
10. The compound according to claim 5, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetB.
11. The compound according to claim 12, or a pharmaceutically
acceptable salt thereof, wherein HetB is a heteroaromatic selected
from the group consisting of pyrrolyl, pyrazolyl, imidazolyl,
pyridyl, pyrimidinyl, thiazolyl, piperidothiazolyl,
pyrrolidothiazolyl, piperidopyridyl, and pyrrolidopyridyl, wherein
the heteroaromatic ring is optionally substituted with 1 or 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C groups.
12. The compound according to claim 10, or a pharmaceutically
acceptable salt thereof, which is a compound selected from the
group consisting of: ##STR00283## ##STR00284## wherein V, V', V'',
Y, Y' and Z are each independently selected from the group
consisting of H, CH.sub.3, pyrrolidinyl, piperidinyl, piperazinyl,
morpholinyl, thiomorpholinyl, CH.sub.2-pyrrolidinyl,
CH.sub.2-piperidinyl, CH.sub.2-piperazinyl, CH.sub.2-morpholinyl,
CH.sub.2-thiomorpholinyl, NH.sub.2, N(H)CH.sub.3,
N(CH.sub.3).sub.2, CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3 and
CH.sub.2N(CH.sub.3).sub.2; with the proviso that: (i) at least one
of V, V' and V'' is H; and (ii) at least one of Y and Y' is H.
13. The compound according to claim 5, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is AryA.
14. The compound according to claim 13, or a pharmaceutically
acceptable salt thereof, wherein AryA is phenyl which is optionally
substituted with 1 or 2 substituents each of which is independently
C.sub.1-3 alkyl, CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, Cl, Br, F,
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3
alkyl).sub.2, C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl,
OC(O)--C.sub.1-3 alkyl, S(O).sub.2--C.sub.1-3 alkyl,
S(O).sub.2NH.sub.2, S(0).sub.2N(H)--C.sub.1-3 alkyl,
S(O).sub.2N(--C.sub.1-3 alkyl).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl.
15. The compound according to claim 5, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 and R.sup.4 together with
the N atom to which they are both attached form a heterocyclyl
selected from the group consisting of: ##STR00285## wherein the
ring is optionally substituted with 1 or 2 substitutents each of
which is independently C.sub.1-3 alkyl, CF.sub.3, CH.sub.2OH,
CH.sub.2O--C.sub.1-3 alkyl, CH.sub.2OCF.sub.3, CH.sub.2NH.sub.2,
CH.sub.2N(H)--C.sub.1-3 alkyl, CH.sub.2N(--C.sub.1-3 alkyl).sub.2,
O--C.sub.1-3 alkyl, OCF.sub.3, oxo, Cl, Br, F, NH.sub.2,
N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2, C(O)NH.sub.2,
C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3 alkyl).sub.2,
C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl, or
S(O).sub.2--C.sub.1-3 alkyl.
16. The compound according to claim 5, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is AryB.
17. The compound according to claim 16, or a pharmaceutically
acceptable salt thereof, wherein AryB is a bicyclic ring selected
from the group consisting of 1,2,3,4-tetrahydroquinolinyl,
1,2,3,4-tetrahydroisoquinolinyl, 2,3-dihydro-1H-isoindolyl and
2,3-dihydro-1H-indolyl, wherein the bicyclic ring is optionally
substituted with 1 or 2 substituents each of which is independently
C.sub.1-3 alkyl, CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, Cl, Br, F,
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3
alkyl).sub.2, C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl,
OC(O)--C.sub.1-3 alkyl, S(O).sub.2--C.sub.1-3 alkyl,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)--C.sub.1-3 alkyl,
S(O).sub.2N(--C.sub.1-3 alkyl).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl.
18. The compound according to claim 1, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)OR.sup.3.
19. The compound according to claim 18, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetA, CH.sub.2-HetA,
CH.sub.2CH.sub.2-HetA, or AryA.
20. The compound according to claim 19, or a pharmaceutically
acceptable salt thereof, wherein: HetA is a heterocyclic ring
selected from the group consisting of azetidinyl, pyrrolidinyl,
pyrazolidinyl, piperidinyl, piperazinyl, azepanyl, oxazepanyl,
oxazolidinyl, isoxazolidinyl, morpholinyl, and tetrahydropyranyl,
wherein the heterocyclic ring is optionally substituted with 1 or 2
substituents each of which is independently C.sub.1-3 alkyl,
CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, Cl, Br, F,
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3
alkyl).sub.2, C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl,
OC(O)--C.sub.1-3 alkyl, S(O).sub.2--C.sub.1-3 alkyl,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)--C.sub.1-3 alkyl, or
S(O).sub.2N(--C.sub.1-3 alkyl).sub.2; and AryA is phenyl which is
optionally substituted with 1 or 2 substituents each of which is
independently C.sub.1-3 alkyl, CH.sub.2NH.sub.2,
CH.sub.2N(H)--C.sub.1-3 alkyl, CH.sub.2N(--C.sub.1-3 alkyl).sub.2,
O--C.sub.1-3 alkyl, Cl, Br, F, NH.sub.2, N(H)--C.sub.1-3 alkyl,
N(--C.sub.1-3 alkyl).sub.2, C(O)NH.sub.2, C(O)N(H)--C.sub.1-3alkyl,
C(O)N(--C.sub.1-3 alkyl).sub.2, C(O)--C.sub.1-3 alkyl,
C(O)O--C.sub.1-3 alkyl, OC(O)--C.sub.1-3 alkyl,
S(O).sub.2--C.sub.1-3 alkyl, S(O).sub.2NH.sub.2,
S(0).sub.2N(H)--C.sub.1-3 alkyl, S(O).sub.2N(--C.sub.1-3
alkyl).sub.2, pyrrolidinyl, piperidinyl, morpholinyl,
CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl.
21. The compound according to claim 1, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)OR.sup.5.
22. A compound according to claim 1, which is a compound selected
from the group consisting of:
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide;
(2S,5R)--N-[(4S)-azepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]o-
ctane-2-carboxamide;
(2S,5R)--N-[(4R)-azepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]--
octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]-octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(3S)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]-octane-2-carboxamide;
(2S,5R)--N-azocan-5-yl-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-
-carboxamide;
(2S,5R)-7-oxo-N-pyridin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-
-carboxamide;
(2S,5R)--N-(2-methoxypyridin-4-yl)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)--N-[2-(dimethylamino)pyridin-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[4-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-2-[(piperidin-4-ylamino)carbonyl]-1,6-diazabicyclo[3.2.1]oc-
tane-6-sulfonic acid;
(4R,6S)-2-oxo-N-piperidin-4-yl-3-(sulfooxy)-1,3-diazabicyclo[2.2.1]-hepta-
ne-6-carboxamide;
(4R,6S)-2-oxo-N-[(4S)-azepan-4-yl]-3-(sulfooxy)-1,3-diazabicyclo[2.2.1]-h-
eptane-6-carboxamide;
(4R,6S)-2-oxo-N-pyridin-4-yl-3-(sulfooxy)-1,3-diazabicyclo[2.2.1]heptane--
6-carboxamide; and pharmaceutically acceptable salts thereof.
23. A compound according to claim 1, which is a compound selected
from the group consisting of:
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide;
(2S,5R)--N-[(3R,4S)-3-fluoropiperidin-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazab-
icyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-6-(sulfooxy)-N-(1,2,3,4-tetrahydroisoquinolin-6-yl)-1,6-dia-
zabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(5-piperidin-4-ylpyridin-2-yl)-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide;
piperidin-4-ylmethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oct-
ane-2-carboxylate; Diastereomer 1 of
(2S,5R)-7-oxo-N-[(3)-piperidin-3-yl]-6-(sulfooxy)-1,6-diaza-bicyclo[3.2.1-
]octane-2-carboxamide; Diastereomer 2 of
(2S,5R)-7-oxo-N-[(3)-piperidin-3-yl]-6-(sulfooxy)-1,6-diaza-bicyclo[3.2.1-
]octane-2-carboxamide;
(2S,5R)-7-oxo-N-azetidin-3-yl-6-(sulfooxy)-1,6-diaza-bicyclo[3.2.1]octane-
-2-carboxamide;
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diaza-bicyclo[3.2-
.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(4R)-azepan-4-yl]-6-(sulfooxy)-1,6-diaza-bicyclo[3.2.1]o-
ctane-2-carboxamide;
(2S,5R)-7-oxo-N-[1-methylpiperidin-4-yl]-6-(sulfooxy)-1,6-diaza-bicyclo[3-
.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(3S,4S)-3-fluoropiperidin-4-yl]-6-(sulfooxy)-1,6-diaza-b-
icyclo[3.2.1]octane-2-carboxamide or its 3R,4R diastereomer or a
mixture thereof;
(2S,5R)-7-oxo-N-[(3S,4R)-3-fluoropiperidin-4-yl]-6-(sulfooxy)-1,-
6-diaza-bicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-[1,1-dioxidotetrahydro-2H-thiopyran-4-yl]-6-(sulfooxy)-1,-
6-diaza-bicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(1,1-dioxidotetrahydro-2H-thiopyran-4-yl)-7-oxo-6-(sulfooxy)-1-
,6-diazabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3R,4R)-4-aminopyrrolidin-3-yl]-7-oxo-6-(sulfooxy)-1,6-diazab-
icyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3R,4R)-4-hydroxypyrrolidin-3-yl]-7-oxo-6-(sulfooxy)-1,6-diaz-
abicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3R,4S)-4-hydroxypyrrolidin-3-yl]-7-oxo-6-(sulfooxy)-1,6-diaz-
abicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3R,4S)-4-fluoropyrrolidin-3-yl]-7-oxo-6-(sulfooxy)-1,6-diaza-
bicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3S,4R)-4-fluoropyrrolidin-3-yl]-7-oxo-6-(sulfooxy)-1,6-diaza-
bicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(3S)-1-piperidin-4-yl-2-oxopyrrolidin-3-yl]-6-(sulfooxy)-
-1,6-diazabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(3R)-1-piperidin-4-yl-2-oxopyrrolidin-3-yl]-6-(sulfooxy)-
-1,6-diazabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3S,4R)-3-fluoroazepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3R,4S)-3-fluoroazepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(3-fluoroazetidin-3-yl)methyl]-7-oxo-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(pyrrolidin-2-ylmethyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(piperidin-2-ylmethyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(piperidin-4-ylmethyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide;
(2S,5R)--N-(2-hydroxy-1-piperidin-4-ylethyl)-7-oxo-6-(sulfooxy)-1,6-diaza-
bicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(2S)-1,4-oxazepan-2-ylmethyl]-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[(2R)-1,4-oxazepan-2-ylmethyl]-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(2-piperidin-4-ylethyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(2-piperidin-1-ylethyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(2-piperazin-1-ylethyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxamide;
(2S,5R)--N-3-azabicyclo[3.1.0]hex-6-yl-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxamide;
(2S,5R)--N-methyl-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)-2-{[2-(aminomethyl)piperidin-1-yl]carbonyl}-6-(sulfooxy)-1,6-diaz-
abicyclo[3.2.1]octan-7-one;
(2S,5R)-2-[(4-aminopiperidin-1-yl)carbonyl]-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octan-7-one;
(2S,5R)-2-(piperazin-1-ylcarbonyl)-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oc-
tan-7-one;
(2S,5R)-2-(2,7-diazaspiro[3.5]non-2-ylcarbonyl)-6-(sulfooxy)-1,-
6-diazabicyclo[3.2.1]octan-7-one;
(2S,5R)-2-(hexahydropyrrolo[3,4-c]pyrrol-2(1H)-ylcarbonyl)-6-(sulfooxy)-1-
,6-diazabicyclo[3.2.1]octan-7-one;
(2S,5R)-2-{[(3R)-3-aminopyrrolidin-1-yl]carbonyl}-6-(sulfooxy)-1,6-diazab-
icyclo[3.2.1]octan-7-one;
(2S,5R)-2-{[(3S)-3-aminopyrrolidin-1-yl]carbonyl}-6-(sulfooxy)-1,6-diazab-
icyclo[3.2.1]octan-7-one;
(2S,5R)-2-{[3-(dimethylamino)pyrrolidin-1-yl]-carb-onyl}-6-(sulfooxy)-1,6-
-diazabicyclo[3.2.1]octan-7-one;
(2S,5R)--N-[4-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)--N-[3-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)--N-[2-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)--N-{4-[(methylamino)methyl]phenyl}-7-oxo-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-{3-[(methylamino)methyl]phenyl}-7-oxo-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-{4-[(dimethylamino)methyl]phenyl}-7-oxo-6-(sulfooxy)-1,6-diaza-
bicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-{4-[(pyrrolidinyl)methyl]phenyl}-7-oxo-6-(sulfooxy)-1,6-diazab-
icyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-6-(sulfooxy)-N-(1,2,3,4-tetrahydroisoquinolin-6-yl)-1,6-dia-
zabicyclo-[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(2,3-dihydro-1H-isoindol-5-yl)-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(2,3-dihydro-1H-indol-5-yl)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxamide;
4-({[(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-[3.2.1]oct-2-yl]carbonyl-
}amino)benzoic acid;
(2S,5R)--N-[4-(aminocarbonyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[-
3.2.1]octane-2-carboxamide;
(2S,5R)--N-[4-(aminosulfonyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[-
3.2.1]octane-2-carboxamide;
(2S,5R)--N-[3-(aminocarbonyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[-
3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-pyridin-3-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-
-carboxamide;
(2S,5R)-7-oxo-N-pyridin-2-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-
-carboxamide;
(2S,5R)--N-(2,6-dipyrrolidin-1-ylpyridin-4-yl)-7-oxo-6-(sulfooxy)-1,6-dia-
zabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(6-aminopyridin-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide;
(2S,5R)--N-[4-(dimethylamino)pyridin-2-yl]-7-oxo-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[4-(aminomethyl)pyridin-2-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-[5-(aminomethyl)pyridin-2-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(4-piperidin-4-ylpyridin-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(6-piperidin-4-ylpyridin-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(5-piperazin-1-ylpyridin-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(5-morpholin-4-ylpyridin-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(5-pyrrolidin-1-yl-pyridin-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazab-
icyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-pyrazin-2-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-
-carboxamide;
(2S,5R)-7-oxo-N-pyrimidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide;
(2S,5R)-7-oxo-N-(2-piperazin-1-ylpyrimidin-4-yl)-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(1-methyl-1H-imidazol-2-yl)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(1-methyl-4,5-dihydro-1H-imidazol-2-yl)-7-oxo-6-(sulfooxy)-1,6-
-diazabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-6-(sulfooxy)-N-1,3-thiazol-2-yl-1,6-diazabicyclo[3.2.1]octa-
ne-2-carboxamide;
(2S,5R)-7-oxo-6-(sulfooxy)-N-(4,5,6,7-tetrahydro[1,3]thiazolo[5,4-c]pyrid-
in-2-yl)-1,6-diazabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)--N-(6,7-dihydro-5H-pyrrolo[3,4-b]pyridin-2-yl)-7-oxo-6-(sulfooxy)-
-1,6-diazabicyclo[3.2.1]octane-2-carboxamide;
(4S)-azepan-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane--
2-carboxylate;
(4R)-azepan-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane--
2-carboxylate;
piperidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2--
carboxylate;
(3R)-pyrrolidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oct-
ane-2-carboxylate;
(3S)-pyrrolidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oct-
ane-2-carboxylate;
azetidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-c-
arboxylate; (3
S,4R)-3-fluoropiperidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3-
.2.1]octane-2-carboxylate;
(3R,4S)-3-fluoropiperidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octane-2-carboxylate;
(3R,4R)-3-fluoropiperidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octane-2-carboxylate;
(3S,4S)-3-fluoropiperidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octane-2-carboxylate;
(3S,4S)-4-fluoropiperidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octane-2-carboxylate;
(3S,4R)-4-fluoropiperidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octane-2-carboxylate;
(3R,4S)-4-fluoropyrrolidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxylate;
(3S,4R)-4-fluoropyrrolidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxylate;
(3R,4R)-4-fluoropyrrolidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxylate;
(3S,4S)-4-fluoropyrrolidin-3-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxylate;
(4S)-isoxazolidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]o-
ctane-2-carboxylate;
(4R)-isoxazolidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]o-
ctane-2-carboxylate;
pyrazolidin-4-yl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane--
2-carboxylate;
2-aminoethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-ca-
rboxylate;
2-piperidin-1-ylethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]octane-2-carboxylate;
2-piperidin-4-ylethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oc-
tane-2-carboxylate;
(4-methylpiperidin-4-yl)methyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo-
[3.2.1]octane-2-carboxylate;
1,4-oxazepan-2-ylmethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]-
octane-2-carboxylate;
4-(aminomethyl)phenyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oc-
tane-2-carboxylate;
2-{[3,4-bis(acetyloxy)benzoyl]amino}ethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-d-
iazabicyclo[3.2.1]octane-2-carboxylate;
2-{[3,4-bis(acetyloxy)benzoyl]amino}-1-{{[3,4-bis(acetyloxy)benzoyl]amino-
}methyl)ethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2-ca-
rboxylate; and pharmaceutically acceptable salts thereof.
24. A compound according to claim 1, which is a compound selected
from the group consisting of:
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide;
(2S,5R)--N-[4-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide;
(2S,5R)-7-oxo-6-(sulfooxy)-N-(1,2,3,4-tetrahydroisoquinolin-6-yl)-1,6-dia-
zabicyclo[3.2.1]octane-2-carboxamide;
(2S,5R)-7-oxo-N-(5-piperidin-4-ylpyridin-2-yl)-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide;
piperidin-4-ylmethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oct-
ane-2-carboxylate; and pharmaceutically acceptable salts
thereof.
25. A compound according to claim 24, which is
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide or a pharmaceutically acceptable salt thereof.
26. A compound according to claim 24, which is
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide or a pharmaceutically acceptable salt
thereof.
27. A compound according to claim 1, which is which is
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide in the form of a crystalline monohydrate.
28. A pharmaceutical composition which comprises a compound
according to any one of claims 1 to 27, or a pharmaceutically
acceptable salt thereof, and a pharmaceutically acceptable
carrier.
29. A pharmaceutical composition according to claim 28, which
further comprises a beta-lactam antibiotic.
30. A method for treating a bacterial infection which comprises
administering to a subject in need of such treatment (i) a
therapeutically effective amount of a compound according to any one
of claims 1 to 27, or a pharmaceutically acceptable salt thereof,
optionally in combination with a beta-lactam antibiotic or (ii) a
pharmaceutical composition according to any claim 27 or claim
28.
31. Use of a compound of a compound according to any one of claims
1 to 27, or a pharmaceutically acceptable salt thereof, optionally
in combination with a beta lactam antibiotic, in the manufacture of
a medicament for treating a bacterial infection.
32. A process for preparing a compound of Formula P-II:
##STR00286## which comprises: (A) contacting a ketosulfoxonium
glide of Formula P-I: ##STR00287## with an iridum, rhodium, or
ruthenium catalyst to obtain Compound P-II; wherein: P.sup.G is an
amine protective group selected from the group consisting of
carbamates and benzylamines; R.sup.U is CH.sub.3 or phenyl; R.sup.V
is CH.sub.3 or phenyl; R.sup.4 is H or C.sub.1-4 alkyl; T' is H,
Cl, Br, F, C.sub.1-3 alkyl, O--C.sub.1-3 alkyl, OH, NH.sub.2,
N(H)--C.sub.1-3 alkyl, or N(--C.sub.1-3 alkyl).sub.2; p is zero, 1
or 2; q is zero, 1, or 2; and p+q=zero, 1, 2, or 3.
33. The process according to claim 32, wherein P.sup.G is Cbz and
Compound P-II is Compound P-IIa: ##STR00288## and wherein the
process further comprises: (B) treating Compound P-IIa with a
reducing agent to obtain a compound of Formula P-III: ##STR00289##
and (C) contacting Compound P-III with a sulfonyl halide of formula
R --SO.sub.2W in the presence of a tertiary amine base to obtain a
compound of Formula P-IV: ##STR00290## wherein: W is halogen; and R
is: (1) phenyl optionally substituted with from 1 to 3 substituents
each of which is independently C.sub.1-4 alkyl, C.sub.1-4
haloalkyl, O--C.sub.1-4 alkyl, O--C.sub.1-4 haloalkyl, Cl, Br, F,
or NO.sub.2; (2) C.sub.1-4 alkyl; or (3) C.sub.1-4 haloalkyl.
34. The process according to claim 33, which further comprises: (D)
contacting Compound P-IV with N-Boc-O-benzylhydroxylamine in the
presence of a base to obtain a compound of Formula P-V:
##STR00291## and (E) treating Compound P-V with an acid to obtain a
compound of Formula P-VI: ##STR00292##
35. The process according to claim 34, which further comprises: (F)
contacting Compound P-VI with phosgene, diphosgene or triphosgene
in the presence of a tertiary amine, and then adding an aqueous
solution of acid to obtain a compound of Formula P-VII:
##STR00293## and (G) contacting Compound P-VII with a source of
hydrogen in the presence of a hydrogenolysis catalyst and in the
presence of a Boc-producing agent to obtain a compound of Formula
P-VIII: ##STR00294##
36. A process according to claim 32, wherein the compound of
Formula P-II is Compound p-2: ##STR00295## which comprises: (A)
contacting ketosulfoxonium ylide p-1: ##STR00296## with a catalyst
selected from the group consisting of iridium cyclooctadiene
chloride dimer, RuCl.sub.2(PPh.sub.3), Ru(DMSO).sub.4Cl.sub.2, and
Rh.sub.2(TFA).sub.4, to obtain Compound p-2.
37. The process according to claim 36, which further comprises: (B)
treating Compound p-2 with a reducing agent selected from the group
consisting of Li borohydride, Na borohydride and K borohydride, to
obtain Compound p-3: ##STR00297## and (C) contacting Compound p-3
with a sulfonyl halide of formula R --SO.sub.2W in the presence of
a tri-C.sub.1-4 alkylamine base to obtain a compound of Formula
p-4: ##STR00298## wherein W is chlorine; and R is methyl,
chloromethyl, phenyl, 4-bromophenyl, 4-trifluoromethylphenyl, or
4-methylphenyl.
38. The process according to claim 37, which further comprises: (D)
contacting Compound p-4 with N-Boc-O-benzylhydroxylamine in the
presence of a a base selected from the group consisting of Li
t-butoxide, Na t-butoxide, K t-butoxide and K amyloxide to obtain
Compound p-5: ##STR00299## and (E) treating Compound p-5 with an
acid selected from the group consisting of methanesulfonic acid,
chloromethanesulfonic acid, p-toluenesulfonic acid and
benzenesulfonic acid to obtain a compound of Formula p-6:
##STR00300##
39. The process according to claim 38, which further comprises: (F)
contacting Compound p-6 with triphosgene in the presence of a
tri-C.sub.1-4 alkylamine base, and then adding an aqueous solution
of phosphoric acid to obtain Compound p-7: ##STR00301## and (G)
contacting Compound p-7 with hydrogen in the presence of a Pd
catalyst and a Boc-producing agent selected from the group
consisting of di-t-butylcarbonate and Boc-ON to obtain Compound
p-8: ##STR00302##
40. A compound selected from the group consisting of: ##STR00303##
wherein: P.sup.G is an amine protective group selected from the
group consisting of carbamates and benzylamines; R.sup.U is
CH.sub.3 or phenyl; R.sup.V is CH.sub.3 or phenyl; R.sup.4 is H or
C.sub.1-4 alkyl; T' is H, Cl, Br, F, C.sub.1-3 alkyl, O--C.sub.1-3
alkyl, OH, NH.sub.2, N(H)--C.sub.1-3 alkyl, or N(--C.sub.1-3
alkyl).sub.2; p is zero, 1 or 2; q is zero, 1, or 2; p+q=zero, 1,
2, or 3; and R is: (1) phenyl optionally substituted with from 1 to
3 substituents each of which is independently C.sub.1-4 alkyl,
C.sub.1-4 haloalkyl, O--C.sub.1-4 alkyl, O--C.sub.1-4 haloalkyl,
Cl, Br, F, or NO.sub.2; (2) C.sub.1-4 alkyl; or (3) C.sub.1-4
haloalkyl.
41. A compound according to claim 40, which is selected from the
group consisting of: ##STR00304## wherein R is methyl,
chloromethyl, phenyl, 4-bromophenyl, 4-trifluoromethylphenyl, or
4-methylphenyl.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional
Application No. 61/011,533 (filed Jan. 18, 2008), the disclosure of
which is hereby incorporated by reference in its entirety.
FIELD OF THE INVENTION
[0002] This invention relates to novel beta-lactamase inhibitors
and their use against bacterial antibiotic resistance. More
particularly, the invention relates to compositions and methods for
overcoming bacterial antibiotic resistance.
BACKGROUND OF THE INVENTION
[0003] Bacterial antibiotic resistance has become one of the most
serious threats to modern health care. Cohen, Science 1992, 257:
1051-1055 discloses that infections caused by resistant bacteria
frequently result in longer hospital stays, higher mortality and
increased cost of treatment. Neu, Science 1992, 257: 1064-1073
discloses that the need for new antibiotics will continue to
escalate because bacteria have a remarkable ability to develop
resistance to new agents rendering them quickly ineffective.
Anderson, Nature America 1999, 5: 147-149 refers to the spread of
antibiotic resistance as a pandemic and asserts that a solution to
the growing public health threat will require an interdisciplinary
approach.
[0004] The present crisis has prompted various efforts to elucidate
the mechanisms responsible for bacterial resistance, Coulton et
al., Progress in Medicinal Chemistry 1994, 31: 297-349 teaches that
the widespread use of penicillins and cephalosporins has resulted
in the emergence of .beta.-lactamases, a family of bacterial
enzymes that catalyze the hydrolysis of the .beta.-lactam ring
common to numerous presently used antibiotics. More recently,
Dudley, Pharmacotherapy 1995, 15: 9S-14S has disclosed that
resistance mediated by .beta.-lactamases is a critical aspect at
the core of the development of bacterial antibiotic resistance.
Clavulanic acid, which is a metabolite of Streptomyces
clavuligerus, and two semi-synthetic inhibitors, sulbactam and
tazobactam are presently available semi-synthetic or natural
product .beta.-lactamase inhibitors. U.S. Pat. No. 5,698,577, U.S.
Pat. No. 5,510,343, U.S. Pat. No. 6,472,406 and Hubschwerlen et
al., J. Med. Chem. 1998, 41: 3961 and Livermore et al., J. Med.
Chem. 1997, 40: 335-343, disclose certain synthetic
.beta.-lactamase inhibitors.
[0005] Other references of interest are:
[0006] US 2003/0199541 A1 discloses certain azabicyclic compounds
including certain 7-oxo-6-diazabicyclic[3.2.1]octane-2-carboxamides
and their use as anti-bacterial agents.
[0007] US 2004/0157826 A1 discloses certain heterobicyclic
compounds including certain diazepine carboxamide and diazepine
carboxylate derivatives and their use as anti-bacterials and
.beta.-lactamase inhibitors.
[0008] WO 2008/039420 A2 discloses certain
7-oxo-2,6-diazabicyclo[3.2.0]heptane-6-sulfooxy-2-carboxamides and
their use of beta-lactamse inhibitors.
[0009] Poole, Cell. Mol. Life. Sci. 2004, 61: 2200-2223, provides a
review of the resistance of bacterial pathogens to .beta.-lactam
antibiotics and approaches for overcoming resistance.
[0010] The currently available .beta.-lactamase inhibitors are
insufficient to counter the constantly increasing diversity of
.beta.-lactamases. There is, therefore, a need for new
.beta.-lactamase inhibitors.
SUMMARY OF THE INVENTION
[0011] The present invention is directed to certain diazabicyclic
carboxamide and carboxylate compounds which are beta-lactamase
inhibitors. The compounds and their pharmaceutically acceptable
salts, are useful in combination with beta-lactam antibiotics for
the treatment of bacterial infections, particularly anti-biotic
resistant bacterial infections. More particularly, the present
invention includes compounds of Formula I:
##STR00002##
and pharmaceutically acceptable salts thereof, wherein: the bond
identified as "a" is a single bond or a double bond; when bond a is
a single bond, X is:
[0012] (1) CH.sub.2,
[0013] (2) CH.sub.2CH.sub.2,
[0014] (3) CH.sub.2CH.sub.2CH.sub.2,
[0015] (4) CH.dbd.CH,
[0016] (5) CH.sub.2--CH.dbd.CH, or
[0017] (6) CH.dbd.CH--CH.sub.2;
when bond a is a double bond, X is:
[0018] (1) CH,
[0019] (2) CH--CH.sub.2, or
[0020] (3) CH--CH.dbd.CH;
R.sup.1 is:
[0021] (1) C(O)N(R.sup.3)R.sup.4,
[0022] (2) C(O)OR.sup.3, or
[0023] (3) C(O)OR.sup.5;
R.sup.2 is SO.sub.3M, OSO.sub.3M, SO.sub.2NH.sub.2, PO.sub.3M,
OPO.sub.3M, CH.sub.2CO.sub.2M, CF.sub.2CO.sub.2M, or CF.sub.3; M is
H or a pharmaceutically acceptable cation;
R.sup.3 is:
[0024] (1) C.sub.1-8 alkyl substituted with a total of from 1 to 4
substituents selected from the group consisting of (i) zero to 2
N(R.sup.A)R.sup.B, (ii) zero to 2 R.sup.C, and (iii) zero to 1 of
AryA, HetA, or HetB, [0025] (2) CycA, [0026] (3) HetA, [0027] (4)
AryA, [0028] (5) HetB, or [0029] (6) AryB; R.sup.4 is H or
C.sub.1-8 alkyl optionally substituted with N(R.sup.A)R.sup.B; or
alternatively, when R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 and
R.sup.4 together with the N atom to which they are both attached
form a 4- to 9-membered, saturated monocyclic ring optionally
containing 1 heteroatom in addition to the nitrogen attached to
R.sup.3 and R.sup.4 selected from N, O, and S, where the S is
optionally oxidized to S(O) or S(O).sub.2; wherein the monocyclic
ring is optionally fused to, bridged with, or spiro to a 4- to
7-membered, saturated heterocyclic ring containing from 1 to 3
heteroatoms independently selected from N, O and S, where the S is
optionally oxidized to S(O) or S(O).sub.2, to form a bicyclic ring
system, wherein the monocyclic ring or the bicyclic ring system so
formed is optionally substituted with 1 or 2 substituents each of
which is independently: (1) C.sub.1-6 alkyl, (2) C.sub.1-6
fluoroalkyl, (3) (CH.sub.2).sub.1-2G wherein G is OH, O--C.sub.1-6
alkyl, O--C.sub.1-6 fluoroalkyl, N(R.sup.A)R.sup.B,
C(O)N(R.sup.A)R.sup.B, C(O)R.sup.A, CO.sub.2R.sup.A, or
SO.sub.2R.sup.A, (4) O--C.sub.1-6 alkyl, (5) O--C.sub.1-6
fluoroalkyl, (6) OH, (7) oxo, (8) halogen, (9) N(R.sup.A)R.sup.B,
(10) C(O)N(R.sup.A)R.sup.B, (11) C(O)R.sup.A, (12) C(O)--C.sub.1-6
fluoroalkyl, (13) C(O)OR.sup.A, or (14) S(O).sub.2R.sup.A; R.sup.5
is C.sub.1-8 alkyl substituted with 1 or 2 substituents each of
which is independently N(R.sup.A)C(O)-AryA; CycA is C.sub.4-9
cycloalkyl which is optionally substituted with a total of from 1
to 4 substituents selected from zero to 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; HetA is a 4- to 9-membered saturated or
mono-unsaturated heterocyclic ring containing from 1 to 3
heteroatoms independently selected from N, O and S, wherein any
ring S is optionally oxidized to S(O) or S(O).sub.2 and either 1 or
2 ring carbons are optionally oxidized to C(O); wherein the ring is
optionally fused with a C.sub.3-7 cycloalkyl; and wherein the
optionally fused, saturated or mono-unsaturated heterocyclic ring
is optionally substituted with a total of from 1 to 4 substituents
selected from zero to 2 (CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero
to 2 (CH.sub.2).sub.nR.sup.C; AryA is phenyl which is optionally
substituted with a total of from 1 to 4 substituents selected from
zero to 2 (CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; HetB is a 5- or 6-membered heteroaromatic
ring containing from 1 to 4 heteroatoms selected from 1 to 3 N
atoms, zero or 1 O atom, and zero or 1 S atom; wherein the
heteroaromatic ring is optionally fused with a 5- to 7-membered,
saturated heterocyclic ring containing 1 or 2 heteroatoms
independently selected from N, O and S, wherein any ring S is
optionally oxidized to S(O) or S(O).sub.2 and either 1 or 2
non-fused ring carbons are optionally oxidized to C(O); and wherein
the optionally fused heteroaromatic ring is optionally substituted
with a total of from 1 to 4 substituents selected from zero to 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; AryB is a bicyclic ring system which is
phenyl fused with a 5- to 7-membered saturated heterocyclic ring
containing from 1 to 3 heteroatoms independently selected from N, O
and S, wherein any ring S is optionally oxidized to S(O) or
S(O).sub.2, and wherein the bicyclic ring system is optionally
substituted with a total of from 1 to 4 substituents selected from
zero to 2 (CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; each n is independently an integer which
is 0, 1, 2, or 3; each R.sup.A is independently H or C.sub.1-8
alkyl; each R.sup.B is independently H or C.sub.1-8 alkyl; each
R.sup.C is independently C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl,
OC(O)--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2,
halogen, CN, C(O)R.sup.A, C(O)OR.sup.A, C(O)N(R.sup.A)R.sup.B,
SO.sub.2R.sup.A, SO.sub.2N(R.sup.A)R.sup.B, pyridyl, pyrrolidinyl,
piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl; and
provided that: [0030] (A) when R.sup.1 is C(O)OR.sup.3 and R.sup.3
is AryA, then AryA is not (i) unsubstituted phenyl, (ii) phenyl
substituted with NH.sub.2, (iii) phenyl substituted with OH, (iii)
phenyl substituted with O--C.sub.1-6 alkyl, (iv) phenyl substituted
with one or more halogens, or (v) phenyl substituted with C.sub.1-6
alkyl; [0031] (B) when R.sup.1 is C(O)OR.sup.3 and R.sup.3 is
C.sub.1-6 alkyl substituted with HetB, then HetB is not pyridyl;
[0032] (C) when R.sup.1 is C(O)OR.sup.3 and R.sup.3 is
CH.sub.2-AryA or CH.sub.2CH.sub.2-AryA, then AryA is not (i)
unsubstituted phenyl, (ii) phenyl substituted with NH.sub.2, OH,
O--C.sub.1-6 alkyl, or C.sub.1-6 alkyl, or (iii) phenyl substituted
with one or more halogens; [0033] (D) when R.sup.1 is
C(O)N(R.sup.3)R.sup.4, R.sup.3 is AryA, CH.sub.2-AryA or
CH.sub.2CH.sub.2-AryA, and R.sup.4 is H or C.sub.1-6 alkyl, then
AryA is not unsubstituted phenyl, phenyl substituted with
N(CH.sub.3).sub.2, or phenyl substituted with C(O)NH.sub.2; [0034]
(E) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 is C.sub.1-6
alkyl substituted with HetB, and R.sup.4 is H or C.sub.1-6 alkyl,
then HetB is not pyridyl; and [0035] (F) when R.sup.1 is
C(O)OR.sup.3 and R.sup.3 is C.sub.1-6 alkyl substituted with
R.sup.C, then R.sup.C is not C(O)NH.sub.2.
[0036] Compounds of Formula I inhibit .beta.-lactamases and
synergize the antibacterial effects of .beta.-lactam antibiotics
(e.g., imipenem, ceftazidime and piperacillin) against
microorganisms normally resistant to .beta.-lactam antibiotics as a
result of the presence of the .beta.-lactamases. The compounds of
the present invention are effective against class A and class C
.beta.-lactamases and their combination with a beta-lactam
antibiotic, such as imipenem, ceftazidine or piperacillin, can
provide for effective treatment of bacterial infections caused by
class A and class C .beta.-lactamase producing microorganisms.
Accordingly, the present invention includes combinations of a
compound of Formula I with a .beta.-lactam antibiotic suitable for
use against against class C .beta.-lactamase producing bacteria
such as Pseudomonas spp. and against class A .beta.-lactamase
producing bacteria such as Klebsiella spp. The invention also
includes compositions comprising a compound of Formula I or its
pharmaceutically acceptable salt, and a pharmaceutically acceptable
carrier. The invention further includes methods for treating
bacterial infections and inhibiting bacterial growth by use of a
compound of Formula I or its salt or a combination or composition
containing the compound or its salt.
[0037] Embodiments, sub-embodiments, aspects and features of the
present invention are either further described in or will be
apparent from the ensuing description, examples and appended
claims.
BRIEF DESCRIPTION OF THE DRAWINGS
[0038] FIG. 1 is the X-ray powder diffraction pattern for the
crystalline monohydrate described in Example 1D.
[0039] FIG. 2 is the DSC curve for the crystalline monohydrate
described in Example 1D.
DETAILED DESCRIPTION OF THE INVENTION
[0040] As noted above the present invention includes compounds of
Formula I, wherein the compounds are beta-lactamase inhibitors
suitable for use in combination with beta-lactam antibiotics for
the treatment of bacterial infections.
[0041] The term ".beta.-lactamase inhibitor" refers to a compound
which is capable of inhibiting .beta.-lactamase activity Inhibiting
.beta.-lactamase activity means inhibiting the activity of a class
A, C, or D .beta.-lactamase. For antimicrobial applications
inhibition at a 50% inhibitory concentration is preferably achieved
at or below about 100 micrograms/mL, or at or below about 50
micrograms/mL, or at or below about 25 micrograms/mL. The terms
"class A", "class C", and "class D" .beta.-lactamases are
understood by those skilled in the art and are described in Waley,
The Chemistry of .beta.-lactamase, Page Ed., Chapman & Hall,
London, (1992) 198-228.
[0042] The term ".beta.-lactamase" denotes a protein capable of
inactivating a .beta.-lactam antibiotic. The .beta.-lactamase can
be an enzyme which catalyzes the hydrolysis of the .beta.-lactam
ring of a .beta.-lactam antibiotic. Of particular interest herein
are microbial .beta.-lactamases. The .beta.-lactamase can be, for
example, a serine .beta.-lactamase. .beta.-Lactamases of interest
include those disclosed in, e.g., Waley, The Chemistry of
.beta.-lactamase, Page Ed., Chapman & Hall, London, (1992)
198-228. .beta.-Lactamases of particular interest herein include a
class C .beta.-lactamase of Pseudomonas aeruginosa or of
Enterobacter cloacae P99 (hereinafter P99.beta.-lactamase) and
class A beta-lactamase of Klebsiella spp.
[0043] The term "antibiotic" refers to a compound or composition
which decreases the viability of a microorganism, or which inhibits
the growth or proliferation of a microorganism. The phrase
"inhibits the growth or proliferation" means increasing the
generation time (i.e., the time required for the bacterial cell to
divide or for the population to double) by at least about 2-fold.
Preferred antibiotics are those which can increase the generation
time by at least about 10-fold or more (e.g., at least about
100-fold or even indefinitely, as in total cell death). As used in
this disclosure, an antibiotic is further intended to include an
antimicrobial, bacteriostatic, or bactericidal agent. Examples of
antibiotics suitable for use with respect to the present invention
include penicillins, cephalosporins and carbapenems.
[0044] The term ".beta.-lactam antibiotic" refers to a compound
with antibiotic properties that contains a .beta.-lactam
functionality. Non-limiting examples of .beta.-lactam antibiotics
useful with respect to the invention include penicillins,
cephalosporins, penems, carbapenems, and monobactams.
[0045] A first embodiment of the present invention (alternatively
referred to herein as "Embodiment E1") is a compound of Formula I
(alternatively referred to herein as "Compound I") as originally
defined (i.e., as defined in the Summary of the Invention above),
or a pharmaceutically acceptable salt thereof; and provided that:
[0046] (A) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not AryA;
[0047] (B) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not
C.sub.1-8 alkyl substituted with HetB; [0048] (C) when R.sup.1 is
C(O)OR.sup.3, then R.sup.3 is not C.sub.1-8 alkyl substituted with
AryA; [0049] (D) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 is
AryA or C.sub.1-8 alkyl substituted with AryA, and R.sup.4 is H or
C.sub.1-8 alkyl, then AryA is not unsubstituted phenyl, phenyl
substituted with 1 or 2 N(R.sup.A)R.sup.B, or phenyl substituted
with 1 or 2 C(O)N(R.sup.A)R.sup.B; [0050] (E) when R.sup.1 is
C(O)N(R.sup.3)R.sup.4 and R.sup.4 is H or C.sub.1-8 alkyl, then
R.sup.3 is not C.sub.1-8 alkyl substituted with HetB; and [0051]
(F) when R.sup.1 is C(O)OR.sup.3 and R.sup.3 is C.sub.1-8 alkyl
substituted with R.sup.C, then R.sup.C is not
C(O)N(R.sup.A)R.sup.B.
[0052] A second embodiment of the present invention (Embodiment E2)
is a compound of Formula I as originally defined, or a
pharmaceutically acceptable salt thereof; and provided that: [0053]
(A) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not AryA; [0054]
(B) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not C.sub.1-8
alkyl substituted with HetB; [0055] (C) when R.sup.1 is
C(O)OR.sup.3, then R.sup.3 is not C.sub.1-8 alkyl substituted with
AryA; [0056] (D) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, then
R.sup.3 is not AryA or C.sub.1-8 alkyl substituted with AryA;
[0057] (E) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, then R.sup.3 is
not C.sub.1-8 alkyl substituted with HetB; and [0058] (F) when
R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not C.sub.1-8 alkyl
substituted with R.sup.C.
[0059] A third embodiment of the present invention (Embodiment E3)
is a compound of Formula I, or a pharmaceutically acceptable salt
thereof; wherein: [0060] R.sup.1 is: [0061] (1)
C(O)N(R.sup.3)R.sup.4, or [0062] (2) C(O)OR.sup.3; [0063] R.sup.3
is: [0064] (1) C.sub.1-8 alkyl substituted with a total of from 1
to 4 substituents selected from the group consisting of (i) zero to
2 N(R.sup.A)R.sup.B, (ii) zero to 2 R.sup.C, and (iii) zero to 1 of
AryA, HetA, or HetB, [0065] (2) CycA, [0066] (3) HetA, [0067] (4)
AryA, or [0068] (5) HetB; [0069] R.sup.4 is H or C.sub.1-8 alkyl
optionally substituted with N(R.sup.A)R.sup.B; [0070] HetA is a 4-
to 9-membered saturated heterocyclic ring containing from 1 to 3
heteroatoms independently selected from N, O and S, wherein the
saturated heterocyclic ring is optionally substituted with a total
of from 1 to 4 substituents selected from zero to 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and zero to 2
(CH.sub.2).sub.nR.sup.C; [0071] each R.sup.C is independently
C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and [0072] all other variables are as originally
defined; and provided that: [0073] (A) when R.sup.1 is C(O)OR.sup.3
and R.sup.3 is AryA, then AryA is not (i) unsubstituted phenyl,
(ii) phenyl substituted with NH.sub.2, (iii) phenyl substituted
with OH, (iii) phenyl substituted with O--C.sub.1-6 alkyl, (iv)
phenyl substituted with one or more halogens; or (v) phenyl
substituted with C.sub.1-6 alkyl; [0074] (B) when R.sup.1 is
C(O)OR.sup.3 and R.sup.3 is C.sub.1-6 alkyl substituted with HetB,
then HetB is not pyridyl; [0075] (C) when R.sup.1 is C(O)OR.sup.3
and R.sup.3 is CH.sub.2-AryA or CH.sub.2CH.sub.2-AryA, then AryA is
not (i) unsubstituted phenyl, (ii) phenyl substituted with
NH.sub.2, OH, O--C.sub.1-6 alkyl, or C.sub.1-6 alkyl, or (iii)
phenyl substituted with one or more halogens; [0076] (D) when
R.sup.1 is C(O)N(R.sup.3)R.sup.4, R.sup.3 is AryA, CH.sub.2-AryA or
CH.sub.2CH.sub.2-AryA, and R.sup.4 is H or C.sub.1-6 alkyl, then
AryA is neither unsubstituted phenyl nor phenyl substituted with
N(CH.sub.3).sub.2; and [0077] (E) when R.sup.1 is
C(O)N(R.sup.3)R.sup.4, R.sup.3 is C.sub.1-6 alkyl substituted with
HetB, and R.sup.4 is H or C.sub.1-6 alkyl, then HetB is not
pyridyl.
[0078] A fourth embodiment of the present invention (Embodiment E4)
is a compound of Formula I as defined in Embodiment E3, or a
pharmaceutically acceptable salt thereof; and provided that: [0079]
(A) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not AryA; [0080]
(B) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not C.sub.1-6
alkyl substituted with HetB; [0081] (C) when R.sup.1 is
C(O)OR.sup.3, then R.sup.3 is not C.sub.1-6 alkyl substituted with
AryA; [0082] (D) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, then
R.sup.3 is not AryA or C.sub.1-6 alkyl substituted with AryA; and
[0083] (E) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, then R.sup.3 is
not C.sub.1-6 alkyl substituted with HetB.
[0084] A fifth embodiment of the present invention (Embodiment E5)
is a compound of Formula I as defined in Embodiment E3, or a
pharmaceutically acceptable salt thereof; and provided that: [0085]
(A) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not AryA; [0086]
(B) when R.sup.1 is C(O)OR.sup.3, then R.sup.3 is not C.sub.1-6
alkyl substituted with HetB; [0087] (C) when R.sup.1 is
C(O)OR.sup.3, then R.sup.3 is not C.sub.1-6 alkyl substituted with
AryA; [0088] (D) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, then
R.sup.3 is not AryA or C.sub.1-6 alkyl substituted with AryA; and
[0089] (E) when R.sup.1 is C(O)N(R.sup.3)R.sup.4, then R.sup.3 is
not C.sub.1-6 alkyl substituted with HetB.
[0090] A sixth embodiment of the present invention (Embodiment E6)
is a compound of Formula I, or a pharmaceutically acceptable salt
thereof, wherein bond "a" is a single bond; X is --CH.sub.2-- or
--CH.sub.2CH.sub.2--; and all other variables are as originally
defined or as defined in any of the foregoing embodiments.
[0091] A seventh embodiment of the present invention (Embodiment
E7) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein bond "a" is a single bond; X is --CH.sub.2--;
and all other variables are as originally defined or as defined in
any of the foregoing embodiments.
[0092] An eighth embodiment of the present invention (Embodiment
E8) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein bond "a" is a single bond; X is
--CH.sub.2CH.sub.2--; and all other variables are as originally
defined or as defined in any of the foregoing embodiments.
[0093] A ninth embodiment of the present invention (Embodiment E9)
is a compound of Formula I, or a pharmaceutically acceptable salt
thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; and all other
variables are as originally defined or as defined in any one of the
preceding embodiments. In an aspect of this embodiment, R.sup.1 is
C(O)NH(R.sup.4).
[0094] A tenth embodiment of the present invention (Embodiment E10)
is a compound of Formula I, or a pharmaceutically acceptable salt
thereof, wherein R.sup.2 is OSO.sub.3M; and all other variables are
as originally defined or as defined in any of the preceding
embodiments.
[0095] An eleventh embodiment of the present invention (Embodiment
E11) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.2 is OSO.sub.3H; and all other
variables are as originally defined or as defined in any of the
preceding embodiments.
[0096] A twelfth embodiment of the present invention (Embodiment
E12) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is: (1) C.sub.1-4 alkyl substituted
with a total of from 1 to 4 substituents selected from the group
consisting of (i) zero to 2 N(R.sup.A)R.sup.B, (ii) zero to 2
R.sup.C, and (iii) zero to 1 of AryA, HetA, or HetB, (2) CycA, (3)
HetA, (4) AryA, or (5) HetB; and all other variables are as
originally defined or as defined in any of the preceding
embodiments.
[0097] A thirteenth embodiment of the present invention (Embodiment
E13) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is
(CH.sub.2).sub.2-3N(R.sup.A)R.sup.B, (CH.sub.2).sub.1-3-AryA,
(CH.sub.2).sub.1-3-HetA, (CH.sub.2).sub.1-3-HetB, CycA, HetA, AryA,
or HetB; and all other variables are as originally defined or as
defined in any of the preceding embodiments.
[0098] A fourteenth embodiment of the present invention (Embodiment
E14) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is HetA, CH.sub.2-HetA,
CH.sub.2CH.sub.2-HetA, CH(CH.sub.3)-HetA, or CH(CH.sub.2OH)-HetA;
and all other variables are as originally defined or as defined in
any of the preceding embodiments. A first sub-embodiment of
Embodiment E14 is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4;
R.sup.3 is as defined above in Embodiment E14; and all other
variables are as defined above in Embodiment E14.
[0099] A fifteenth embodiment of the present invention (Embodiment
E15) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is R.sup.3 is HetA, CH.sub.2-HetA, or
CH.sub.2CH.sub.2-HetA; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E15 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.1 is
C(O)N(R.sup.3)R.sup.4; R.sup.3 is as defined above in Embodiment
E15; and all other variables are as defined above in Embodiment
E15.
[0100] A sixteenth embodiment of the present invention (Embodiment
E16) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is R.sup.3 is HetA or CH.sub.2-HetA;
and all other variables are as originally defined or as defined in
any of the preceding embodiments. A first sub-embodiment of
Embodiment E16 is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4;
R.sup.3 is as defined above in Embodiment E16; and all other
variables are as defined above in Embodiment E16.
[0101] A seventeenth embodiment of the present invention
(Embodiment E17) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetA; and all other
variables are as originally defined or as defined in any of the
preceding embodiments. A first sub-embodiment of Embodiment E17 is
a compound of Formula I, or a pharmaceutically acceptable salt
thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is as
defined above in Embodiment E17; and all other variables are as
defined above in Embodiment E17.
[0102] An eighteenth embodiment of the present invention
(Embodiment E18) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetA; HetA is a
saturated heterocyclic selected from the group consisting of
pyrrolidinyl, piperidinyl, azepanyl, and azocanyl; wherein the
saturated heterocyclic is optionally substituted with
N(R.sup.A)R.sup.B and optionally substituted with 1 or 2
(CH.sub.2).sub.nR.sup.C; each R.sup.C is independently C.sub.1-6
alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and all other variables are as originally defined or
as defined in any of the preceding embodiments.
[0103] A nineteenth embodiment of the present invention (Embodiment
E19) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is HetA; HetA is:
##STR00003##
wherein the asterisk denotes the point of attachment of HetA to the
rest of the compound; T is H or R.sup.C; R.sup.C is C.sub.1-6
alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and all other variables are as originally defined or
as defined in any of the preceding embodiments.
[0104] A twentieth embodiment of the present invention (Embodiment
E20) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is HetA; HetA is:
##STR00004##
wherein the asterisk denotes the point of attachment of HetA to the
rest of the compound; T is H or R.sup.C; R.sup.C is C.sub.1-6
alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and all other variables are as originally defined or
as defined in any of the preceding embodiments. In an aspect of
this embodiment, T is H.
[0105] A twenty-first embodiment of the present invention
(Embodiment E21) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is an optionally fused,
saturated heterocyclic ring selected from the group consisting of
azetidinyl, pyrrolidinyl, oxopyrrolidinyl (e.g.,
2-oxopyrrolidinyl), piperidinyl, piperazinyl, tetrahydropyranyl,
tetrahydrothiopyranyl, morpholinyl,
1,1-dioxidotetrahydrothiopyranyl, azepanyl, oxazepanyl, azocanyl,
and azabicyclo[3.1.0]cyclohexyl, wherein the heterocyclic is
optionally substituted with 1 or 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C; and all other variables are as
originally defined or as defined in any of the preceding
embodiments. A first sub-embodiment of Embodiment E21 is a compound
of Formula I, or a pharmaceutically acceptable salt thereof,
wherein R.sup.3 is HetA, and HetA and all other variables are as
defined above in Embodiment E21. A second sub-embodiment of
Embodiment E21 is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4;
R.sup.3 is HetA; and HetA and all other variables are as defined
above in Embodiment E21. In an aspect of this embodiment and its
sub-embodiments, HetA is optionally mono-substituted with
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C.
[0106] A twenty-second embodiment of the present invention
(Embodiment E22) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is a saturated heterocyclic
ring selected from the group consisting of pyrrolidinyl,
piperidinyl, azepanyl, and azocanyl; wherein the heterocyclic is
optionally substituted with 1 or 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C; and all other variables are as
originally defined or as defined in any of the preceding
embodiments. A first sub-embodiment of Embodiment E22 is a compound
of Formula I, or a pharmaceutically acceptable salt thereof,
wherein R.sup.3 is HetA; and HetA and all other variables are as
defined above in Embodiment E22. A second sub-embodiment of
Embodiment E22 is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4;
R.sup.3 is HetA; and HetA and all other variables are as defined
above in Embodiment E22. In an aspect of this embodiment and its
sub-embodiments, HetA is optionally mono-substituted with
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C.
[0107] A twenty-third embodiment of the present invention
(Embodiment E23) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is as defined in either
Embodiment E21 or E22; each R.sup.C is independently OH,
O--C.sub.1-4 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, Cl,
Br, F, or CN; and all other variables are as originally defined or
as defined in any of the preceding embodiments. In a first
sub-embodiment R.sup.3 is HetA; and HetA is as defined above in
Embodiment E21. In a second sub-embodiment, R.sup.1 is
C(O)N(R.sup.3)R.sup.4; R.sup.3 is HetA; and HetA is as defined
above in Embodiment E22. In an aspect of this embodiment and its
sub-embodiments, HetA is optionally mono-substituted with
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C.
[0108] A twenty-fourth embodiment of the present invention
(Embodiment E24) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is a heterocyclic ring as
defined in either Embodiment E21 or E22; the heterocyclic ring in
HetA is optionally substituted with halogen, C.sub.1-3 alkyl,
O--C.sub.1-3 alkyl, NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3
alkyl).sub.2, CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, or piperidinyl; and all other
variables are as originally defined or as defined in any of the
preceding embodiments. In a first sub-embodiment R.sup.3 is HetA;
and HetA is as defined above in Embodiment E24. In a second
sub-embodiment, R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is HetA;
and HetA is as defined above in Embodiment E24.
[0109] A twenty-fifth embodiment of the present invention
(Embodiment E25) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is a heterocyclic ring as
defined in either Embodiment E21 or E22; the heterocyclic ring in
HetA is optionally substituted with F, CH.sub.3, OCH.sub.3,
NH.sub.2, N(H)CH.sub.3, N(CH.sub.3).sub.2, CH.sub.2NH.sub.2,
CH.sub.2N(H)CH.sub.3, CH.sub.2N(CH.sub.3).sub.2, or piperidinyl;
and all other variables are as originally defined or as defined in
any of the preceding embodiments. In a first sub-embodiment R.sup.3
is HetA; and HetA is as defined above in Embodiment E25. In a
second sub-embodiment, R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is
HetA; and HetA is as defined above in Embodiment E25.
[0110] A twenty-sixth embodiment of the present invention
(Embodiment E26) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is a heterocyclic ring
selected from the group consisting of azetidinyl, pyrrolidinyl,
pyrazolidinyl, piperidinyl, piperazinyl, azepanyl, oxazepanyl,
oxazolidinyl, isoxazolidinyl, morpholinyl, and tetrahydropyranyl,
wherein the heterocyclic ring is optionally substituted with 1 or 2
substituents each of which is independently C.sub.1-3 alkyl,
CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, Cl, Br, F,
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3
alkyl).sub.2, C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl,
OC(O)--C.sub.1-3 alkyl, S(O).sub.2--C.sub.1-3 alkyl,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)--C.sub.1-3 alkyl, or
S(O).sub.2N(--C.sub.1-3 alkyl).sub.2; and all other variables are
as originally defined or as defined in any of the preceding
embodiments. A first sub-embodiment of Embodiment E26 is a compound
of Formula I, or a pharmaceutically acceptable salt thereof,
wherein R.sup.3 is HetA, CH.sub.2-HetA, CH.sub.2CH.sub.2-HetA; and
HetA and all other variables are as defined above in Embodiment
E26. A second sub-embodiment of Embodiment E26 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)OR.sup.3; R.sup.3 is HetA, CH.sub.2-HetA,
CH.sub.2CH.sub.2-HetA; and HetA and all other variables are as
defined above in Embodiment E26. In an aspect of this embodiment
and its sub-embodiments, R.sup.2 is OSO.sub.3H.
[0111] A twenty-seventh embodiment of the present invention
(Embodiment E27) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetA is a heterocyclic ring
selected from the group consisting of azetidinyl, pyrrolidinyl,
pyrazolidinyl, piperidinyl, piperazinyl, azepanyl, oxazepanyl,
oxazolidinyl, isoxazolidinyl, morpholinyl, and tetrahydropyranyl,
wherein the heterocyclic ring is optionally substituted with 1 or 2
substituents each of which is independently CH.sub.3,
CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3, CH.sub.2N(CH.sub.3).sub.2,
OCH.sub.3, Cl, Br, F, NH.sub.2, N(H)CH.sub.3, N(CH.sub.3).sub.2,
C(O)NH.sub.2, C(O)N(H)CH.sub.3, C(O)N(CH.sub.3).sub.2,
C(O)CH.sub.3, C(O)OCH.sub.3, OC(O)CH.sub.3, S(O).sub.2CH.sub.3,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)CH.sub.3, or
S(O).sub.2N(CH.sub.3).sub.2; and all other variables are as
originally defined or as defined in any of the preceding
embodiments. A first sub-embodiment of Embodiment E27 is a compound
of Formula I, or a pharmaceutically acceptable salt thereof,
wherein R.sup.3 is HetA, CH.sub.2-HetA, CH.sub.2CH.sub.2-HetA; and
HetA and all other variables are as defined above in Embodiment
E27. A second sub-embodiment of Embodiment E27 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)OR.sup.3; R.sup.3 is HetA, CH.sub.2-HetA,
CH.sub.2CH.sub.2-HetA; and HetA and all other variables are as
defined above in Embodiment E27. In an aspect of this embodiment
and its sub-embodiments, R.sup.2 is OSO.sub.3H.
[0112] A twenty-eighth embodiment of the present invention
(Embodiment E28) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein AryA is phenyl which is optionally
substituted with 1 or 2 substituents each of which is independently
C.sub.1-3 alkyl, CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, Cl, Br, F,
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3
alkyl).sub.2, C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl,
OC(O)--C.sub.1-3 alkyl, S(O).sub.2--C.sub.1-3 alkyl,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)--C.sub.1-3 alkyl,
S(O).sub.2N(--C.sub.1-3 alkyl).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E28 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryA;
and AryA and all other variables are as defined above in Embodiment
E28. A second sub-embodiment of Embodiment E28 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)OR.sup.3; R.sup.3 is AryA; and AryA and all other
variables are as defined above in Embodiment E28. In an aspect of
this embodiment and its sub-embodiments, R.sup.2 is OSO.sub.3H.
[0113] A twenty-ninth embodiment of the present invention
(Embodiment E29) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein AryA is phenyl which is optionally
substituted with 1 or 2 substituents each of which is independently
CH.sub.3, CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3,
CH.sub.2N(CH.sub.3).sub.2, OCH.sub.3, Cl, Br, F, NH.sub.2,
N(H)CH.sub.3, N(CH.sub.3).sub.2, C(O)NH.sub.2, C(O)N(H)CH.sub.3,
C(O)N(CH.sub.3).sub.2, C(O)CH.sub.3, C(O)OCH.sub.3, OC(O)CH.sub.3,
S(O).sub.2CH.sub.3, S(O).sub.2NH.sub.2, S(O).sub.2N(H)CH.sub.3, or
S(O).sub.2N(CH.sub.3).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E29 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryA;
and AryA and all other variables are as defined above in Embodiment
E29. A second sub-embodiment of Embodiment E29 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)OR.sup.3; R.sup.3 is AryA; and AryA and all other
variables are as defined above in Embodiment E29. In an aspect of
this embodiment and its sub-embodiments, R.sup.2 is OSO.sub.3H.
[0114] A thirtieth embodiment of the present invention (Embodiment
E30) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.3 is HetB; and all other variables are
as originally defined or as defined in any of the preceding
embodiments. A first sub-embodiment of Embodiment E30 is a compound
of Formula I, or a pharmaceutically acceptable salt thereof,
wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is as defined
above in Embodiment E30; and all other variables are as defined
above in Embodiment E30.
[0115] A thirty-first embodiment of the present invention
(Embodiment E31) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetB; HetB is a
heteroaromatic ring selected from the group consisting of pyrrolyl,
pyrazolyl, imidazolyl, pyridyl, and pyrimidinyl, wherein the
heteroaromatic ring is optionally monosubstituted with
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C groups; each R.sup.C is independently
C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and all other variables are as originally defined or
as defined in any of the preceding embodiments.
[0116] A thirty-second embodiment of the present invention
(Embodiment E32) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetB; HetB is pyridyl
which is optionally monosubstituted with N(R.sup.A)R.sup.B and
optionally substituted with 1 or 2 R.sup.C groups; each R.sup.C is
independently C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl,
C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl,
pyrrolidinyl, or piperidinyl; and all other variables are as
originally defined or as defined in any of the preceding
embodiments.
[0117] A thirty-third embodiment of the present invention
(Embodiment E33) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is HetB; HetB is
##STR00005##
U is H, N(R.sup.A)R.sup.B, or R.sup.C; R.sup.C is independently
C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and all other variables are as originally defined or
as defined in any of the preceding embodiments. In an aspect of
this embodiment, U is ortho to the pyridinyl N. In a feature of
this aspect U is H, NH(C.sub.1-4 alkyl), N(C.sub.1-4 alkyl).sub.2,
or O--C.sub.1-4 alkyl.
[0118] A thirty-fourth embodiment of the present invention
(Embodiment E34) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein HetB is a heteroaromatic selected
from the group consisting of pyrrolyl, pyrazolyl, imidazolyl,
pyridyl, pyrimidinyl, thiazolyl, piperidothiazolyl,
pyrrolidothiazolyl, piperidopyridyl, and pyrrolidopyridyl, wherein
the heteroaromatic ring is optionally substituted with 1 or 2
(CH.sub.2).sub.nN(R.sup.A)R.sup.B and optionally substituted with 1
or 2 (CH.sub.2).sub.nR.sup.C groups; and all other variables are as
originally defined or as defined in any of the preceding
embodiments. A first sub-embodiment of Embodiment E34 is a compound
of Formula I, or a pharmaceutically acceptable salt thereof,
wherein R.sup.3 is HetB; and HetB and all other variables are as
defined above in Embodiment E34. A second sub-embodiment of
Embodiment E34 is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4;
R.sup.3 is HetB; and HetB and all other variables are as defined
above in Embodiment E34. In an aspect of this embodiment and its
sub-embodiments, the heteroaromatic in HetB is optionally
mono-substituted with (CH.sub.2).sub.nN(R.sup.A)R.sup.B and
optionally substituted with 1 or 2 (CH.sub.2).sub.nR.sup.C. In
another aspect of this embodiment and its sub-embodiments, the
heteroaromatic ring in HetB is optionally monosubstituted with
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl, or
CH.sub.2N(--C.sub.1-3 alkyl).sub.2; and is optionally substituted
with 1 or 2 substituents each of which is independently C.sub.1-3
alkyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, or
thiomorpholinyl. In still another aspect of this embodiment and its
sub-embodiments, the heteroaromatic ring in HetB is optionally
monosubstituted with NH.sub.2, N(H)CH.sub.3, N(CH.sub.3).sub.2,
CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3, or
CH.sub.2N(CH.sub.3).sub.2; and is optionally substituted with 1 or
2 substituents each of which is independently CH.sub.3,
pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, or
thiomorpholinyl. In still another aspect of this embodiment and its
sub-embodiments, R.sup.2 is OSO.sub.3H.
[0119] A thirty-fifth embodiment of the present invention
(Embodiment E35) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is AryA; and all other
variables are as originally defined or as defined in any of the
preceding embodiments. A first sub-embodiment of Embodiment E35 is
a compound of Formula I, or a pharmaceutically acceptable salt
thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryA;
and AryA and all other variables are as defined above in Embodiment
E35. In an aspect of this embodiment and its sub-embodiment,
R.sup.2 is OSO.sub.3H.
[0120] A thirty-sixth embodiment of the present invention
(Embodiment E36) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein AryA is phenyl which is optionally
substituted with 1 or 2 substituents each of which is independently
C.sub.1-3 alkyl, CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, Cl, Br, F,
NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3 alkyl).sub.2,
C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3
alkyl).sub.2, C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl,
OC(O)--C.sub.1-3 alkyl, S(O).sub.2--C.sub.1-3 alkyl,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)--C.sub.1-3 alkyl,
S(O).sub.2N(--C.sub.1-3 alkyl).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E36 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryA;
and AryA and all other variables are as defined above in Embodiment
E36. A second sub-embodiment of Embodiment E36 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryA; and AryA and all
other variables are as defined above in Embodiment E36. In an
aspect of this embodiment and its sub-embodiments, R.sup.2 is
OSO.sub.3H.
[0121] A thirty-seventh embodiment of the present invention
(Embodiment E37) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein AryA is phenyl which is optionally
substituted with 1 or 2 substituents each of which is independently
CH.sub.3, CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3,
CH.sub.2N(CH.sub.3).sub.2, OCH.sub.3, Cl, Br, F, NH.sub.2,
N(H)CH.sub.3, N(CH.sub.3).sub.2, C(O)NH.sub.2, C(O)N(H)CH.sub.3,
C(O)N(CH.sub.3).sub.2, C(O)CH.sub.3, C(O)OCH.sub.3, OC(O)CH.sub.3,
S(O).sub.2CH.sub.3, S(O).sub.2NH.sub.2, S(O).sub.2N(H)CH.sub.3, or
S(O).sub.2N(CH.sub.3).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E37 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryA;
and AryA and all other variables are as defined above in Embodiment
E37. A second sub-embodiment of Embodiment E37 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryA; and AryA and all
other variables are as defined above in Embodiment E37. In an
aspect of this embodiment and its sub-embodiments, R.sup.2 is
OSO.sub.3H.
[0122] A thirty-eighth embodiment of the present invention
(Embodiment E38) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein AryA is phenyl which is optionally
substituted with 1 or 2 substituents each of which is independently
CH.sub.3, CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3,
CH.sub.2N(CH.sub.32, OCH.sub.3, Cl, Br, F, NH.sub.2, N(H)CH.sub.3,
N(CH.sub.3).sub.2, C(O)NH.sub.2, C(O)N(H)CH.sub.3,
C(O)N(CH.sub.3).sub.2, C(O)CH.sub.3, C(O)OCH.sub.3, OC(O)CH.sub.3,
S(O).sub.2CH.sub.3, S(O).sub.2NH.sub.2, S(O).sub.2N(H)CH.sub.3,
S(O).sub.2N(CH.sub.3).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E38 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryA;
and AryA and all other variables are as defined above in Embodiment
E38. A second sub-embodiment of Embodiment E38 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryA; and AryA and all
other variables are as defined above in Embodiment E37. In an
aspect of this embodiment and its sub-embodiments, R.sup.2 is
OSO.sub.3H.
[0123] A thirty-ninth embodiment of the present invention
(Embodiment E39) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4;
R.sup.3 and R.sup.4 together with the N atom to which they are both
attached form a heterocyclyl selected from the group consisting
of:
##STR00006##
wherein the ring is optionally substituted with 1 or 2
substitutents each of which is independently C.sub.1-3 alkyl,
CF.sub.3, CH.sub.2OH, CH.sub.2O--C.sub.1-3 alkyl,
CH.sub.2OCF.sub.3, CH.sub.2NH.sub.2, CH.sub.2N(H)--C.sub.1-3 alkyl,
CH.sub.2N(--C.sub.1-3 alkyl).sub.2, O--C.sub.1-3 alkyl, OCF.sub.3,
oxo, Cl, Br, F, NH.sub.2, N(H)--C.sub.1-3 alkyl, N(--C.sub.1-3
alkyl).sub.2, C(O)NH.sub.2, C(O)N(H)--C.sub.1-3 alkyl,
C(O)N(--C.sub.1-3 alkyl).sub.2, C(O)--C.sub.1-3 alkyl,
C(O)O--C.sub.1-3 alkyl, or S(O).sub.2--C.sub.1-3 alkyl; and all
other variables are as originally defined or as defined in any of
the preceding embodiments. In an aspect of this embodiment, R.sup.2
is OSO.sub.3H.
[0124] A fortieth embodiment of the present invention (Embodiment
E40) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 and
R.sup.4 together with the N atom to which they are both attached
form a heterocyclyl selected from the group consisting of:
##STR00007##
wherein the ring is optionally substituted with 1 or 2
substitutents each of which is independently CH.sub.3, CF.sub.3,
CH.sub.2OH, CH.sub.2OCH.sub.3, CH.sub.2OCF.sub.3, CH.sub.2NH.sub.2,
CH.sub.2N(H)CH.sub.3, CH.sub.2N(CH.sub.3).sub.2, OCH.sub.3,
OCF.sub.3, oxo, Cl, Br, F, NH.sub.2, N(H)CH.sub.3,
N(CH.sub.3).sub.2, C(O)NH.sub.2, C(O)N(H)CH.sub.3,
C(O)N(CH.sub.3).sub.2, C(O)CH.sub.3, C(O)OCH.sub.3, or
S(O).sub.2CH.sub.3; and all other variables are as originally
defined or as defined in any of the preceding embodiments. In an
aspect of this embodiment, R.sup.2 is OSO.sub.3H.
[0125] A forty-first embodiment of the present invention
(Embodiment E41) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is AryB; and all other
variables are as originally defined or as defined in any of the
preceding embodiments. A first sub-embodiment of Embodiment E41 is
a compound of Formula I, or a pharmaceutically acceptable salt
thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryB;
and AryB and all other variables are as defined above in Embodiment
E41. In an aspect of this embodiment and its sub-embodiment,
R.sup.2 is OSO.sub.3H.
[0126] A forty-second embodiment of the present invention
(Embodiment E42) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is AryB; AryB is a
bicyclic ring selected from the group consisting of
1,2,3,4-tetrahydroquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl,
2,3-dihydro-1H-isoindolyl and 2,3-dihydro-1H-indolyl, wherein the
bicyclic ring is optionally substituted with 1 or 2 substituents
each of which is independently C.sub.1-3 alkyl, CH.sub.2NH.sub.2,
CH.sub.2N(H)--C.sub.1-3 alkyl, CH.sub.2N(--C.sub.1-3 alkyl).sub.2,
O--C.sub.1-3 alkyl, Cl, Br, F, NH.sub.2, N(H)--C.sub.1-3 alkyl,
N(--C.sub.1-3 alkyl).sub.2, C(O)NH.sub.2, C(O)N(H)--C.sub.1-3
alkyl, C(O)N(--C.sub.1-3 alkyl).sub.2, C(O)--C.sub.1-3 alkyl,
C(O)O--C.sub.1-3 alkyl, OC(O)--C.sub.1-3 alkyl,
S(O).sub.2--C.sub.1-3 alkyl, S(O).sub.2NH.sub.2,
S(O).sub.2N(H)--C.sub.1-3 alkyl, S(O).sub.2N(--C.sub.1-3
alkyl).sub.2, pyrrolidinyl, piperidinyl, morpholinyl,
CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E42 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryB;
and AryB and all other variables are as defined above in Embodiment
E42. A second sub-embodiment of Embodiment E42 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryB; and AryB and all
other variables are as defined above in Embodiment E42. In an
aspect of this embodiment and its sub-embodiments, R.sup.2 is
OSO.sub.3H.
[0127] A forty-third embodiment of the present invention
(Embodiment E43) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.3 is AryB; AryB is a
bicyclic ring selected from the group consisting of
1,2,3,4-tetrahydroquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl,
2,3-dihydro-1H-isoindolyl and 2,3-dihydro-1H-indolyl, wherein the
bicyclic ring is optionally substituted with 1 or 2 substituents
each of which is independently CH.sub.3, CH.sub.2NH.sub.2,
CH.sub.2N(H)CH.sub.3, CH.sub.2N(CH.sub.3).sub.2, OCH.sub.3, Cl, Br,
F, NH.sub.2, N(H)CH.sub.3, N(CH.sub.3).sub.2, C(O)NH.sub.2,
C(O)N(H)CH.sub.3, C(O)N(CH.sub.3).sub.2, C(O)CH.sub.3,
C(O)OCH.sub.3, OC(O)CH.sub.3, S(O).sub.2CH.sub.3,
S(O).sub.2NH.sub.2, S(O).sub.2N(H)CH.sub.3, or
S(O).sub.2N(CH.sub.3).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments. A first
sub-embodiment of Embodiment E43 is a compound of Formula I, or a
pharmaceutically acceptable salt thereof, wherein R.sup.3 is AryB,
and AryB and all other variables are as defined above in Embodiment
E43. A second sub-embodiment of Embodiment E43 is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, wherein
R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryB, and AryB and all
other variables are as defined above in Embodiment E43. In an
aspect of this embodiment and its sub-embodiments, R.sup.2 is
OSO.sub.3H.
[0128] A forty-fourth embodiment of the present invention
(Embodiment E44) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.4 is H or C.sub.1-4 alkyl
optionally substituted with N(R.sup.A)R.sup.B; and all other
variables are as originally defined or as defined in any of the
preceding embodiments. In an aspect of this embodiment, R.sup.4 is
H or C.sub.1-4 alkyl.
[0129] A forty-fifth embodiment of the present invention
(Embodiment E45) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.4 is H, C.sub.1-3 alkyl, or
(CH.sub.2).sub.2-3N(R.sup.A)R.sup.B; and all other variables are as
originally defined or as defined in any of the preceding
embodiments. In an aspect of this embodiment, R.sup.4 is H or
C.sub.1-3 alkyl.
[0130] A forty-sixth embodiment of the present invention
(Embodiment E46) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.4 is H or methyl; and all
other variables are as originally defined or as defined in any of
the preceding embodiments.
[0131] A forty-seventh embodiment of the present invention
(Embodiment E47) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein R.sup.4 is H; and all other
variables are as originally defined or as defined in any of the
preceding embodiments.
[0132] A forty-eighth embodiment of the present invention
(Embodiment E48) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein each R.sup.A is independently H or
C.sub.1-4 alkyl; each R.sup.B is independently H or C.sub.1-4
alkyl; and all other variables are as originally defined or as
defined in any of the preceding embodiments.
[0133] A forty-ninth embodiment of the present invention
(Embodiment E49) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein each R.sup.A is independently H or
C.sub.1-3 alkyl; each R.sup.B is independently H or C.sub.1-3
alkyl; and all other variables are as originally defined or as
defined in any of the preceding embodiments.
[0134] A fiftieth embodiment of the present invention (Embodiment
E50) is a compound of Formula I, or a pharmaceutically acceptable
salt thereof, wherein each R.sup.A is independently H or CH.sub.3;
each R.sup.B is independently H or CH.sub.3; and all other
variables are as originally defined or as defined in any of the
preceding embodiments.
[0135] A fifty-first embodiment of the present invention
(Embodiment E51) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein each R.sup.C is independently
C.sub.1-4 alkyl, OH, O--C.sub.1-4 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and all other variables are as originally defined or
as defined in any of the preceding embodiments.
[0136] A fifty-second embodiment of the present invention
(Embodiment E52) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein each R.sup.C is independently OH,
O--C.sub.1-4 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, Cl,
Br, F, or CN; and all other variables are as originally defined or
as defined in any of the preceding embodiments.
[0137] A fifty-third embodiment of the present invention
(Embodiment E53) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein each R.sup.C is independently
C.sub.1-3 alkyl, O--C.sub.1-3 alkyl, Cl, Br, F, C(O)NH.sub.2,
C(O)N(H)--C.sub.1-3 alkyl, C(O)N(--C.sub.1-3 alkyl).sub.2,
C(O)--C.sub.1-3 alkyl, C(O)O--C.sub.1-3 alkyl, OC(O)--C.sub.1-3
alkyl, S(O).sub.2--C.sub.1-3 alkyl, S(O).sub.2NH.sub.2,
S(O).sub.2N(H)--C.sub.1-3 alkyl, S(O).sub.2N(--C.sub.1-3
alkyl).sub.2, pyrrolidinyl, piperidinyl, morpholinyl,
CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments.
[0138] A fifty-fourth embodiment of the present invention
(Embodiment E54) is a compound of Formula I, or a pharmaceutically
acceptable salt thereof, wherein each R.sup.C is independently
CH.sub.3, OCH.sub.3, Cl, Br, F, C(O)NH.sub.2, C(O)N(H)CH.sub.3,
C(O)N(CH.sub.3).sub.2, C(O)CH.sub.3, C(O)OCH.sub.3, OC(O)CH.sub.3,
S(O).sub.2CH.sub.3, S(O).sub.2NH.sub.2, S(O).sub.2N(H)CH.sub.3, or
S(O).sub.2N(CH.sub.3).sub.2, pyrrolidinyl, piperidinyl,
morpholinyl, CH.sub.2-pyrrolidinyl, CH.sub.2-piperidinyl, or
CH.sub.2-morpholinyl; and all other variables are as originally
defined or as defined in any of the preceding embodiments.
[0139] Unless it is expressly stated to the contrary or otherwise
is clear from the context, the provisos A to F set forth in the
definition of Compound I in the Summary of the Invention apply to
the preceding and subsequent embodiments herein. It is clear from
the context, for example, that when any one of Embodiments E11 to
E120 is incorporated into the definition of Compound I as
originally defined, none of the provisos applies. Furthermore, to
the extent any embodiment refers back to and incorporates
Embodiment E1 or Embodiment E2 it includes the provisos A to F set
forth therein, to the extent any of them applies. It is further
understood that the definitions of variables in the provisos can be
customized to reflect the definitions of variables in the
embodiments being incorporated therein. For example, when
Embodiment E9 (i.e., R.sup.1 is C(O)N(R.sup.3)R.sup.4) is
incorporated into Embodiment E1, the proviso can be adjusted to
read as follows (wherein provisos A, B C and F do not apply)--and
provided that: [0140] (D) when R.sup.3 is AryA or C.sub.1-6 alkyl
substituted with AryA, and R.sup.4 is H or C.sub.1-6 alkyl, then
AryA is neither unsubstituted phenyl nor phenyl substituted with 1
or 2 N(R.sup.A)R.sup.B; and [0141] (E) when R.sup.4 is H or
C.sub.1-6 alkyl, then R.sup.3 is not C.sub.1-6 alkyl substituted
with HetB.
[0142] As still another example, when the compound is as defined in
the second sub-embodiment of Embodiment E36 (i.e., R.sup.1 is
C(O)N(R.sup.3)R.sup.4; R.sup.3 is AryA; and AryA and all other
variables are as defined above in Embodiment E36), then it is
understood that the following applies: and provided that AryA is
not unsubstituted phenyl, phenyl substituted with
N(CH.sub.3).sub.2, or phenyl substituted with C(O)NH.sub.2. It is
further understood that the optional provisos set forth in
Embodiments E1 and E2, suitably customized, can alternatively be
applied. The proviso based on Embodiment E1, for example, is: and
provided that AryA is not unsubstituted phenyl, phenyl substituted
with 1 or 2 of NH.sub.2, N(H)--C.sub.1-3 alkyl and N(--C.sub.1-3
alkyl).sub.2, or phenyl substituted with 1 or 2 of C(O)NH.sub.2,
C(O)N(H)--C.sub.1-3 alkyl and C(O)N(--C.sub.1-3 alkyl).sub.2.
[0143] A first class of compounds of the present invention
(alternatively referred to herein as "Class C1") includes compounds
of Formula I and pharmaceutically acceptable salts thereof, wherein
R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is HetA; and all other
variables are as originally defined. In an aspect of this class,
R.sup.4 is H.
[0144] A first sub-class of the first class (alternatively referred
to herein as "Sub-class C1-S1") includes the compounds of Formula I
and pharmaceutically acceptable salts thereof, wherein R.sup.1 is
C(O)N(R.sup.3)R.sup.4; R.sup.3 is HetA; and HetA is a saturated
heterocyclic ring selected from the group consisting of
pyrrolidinyl, piperidinyl, azepanyl, and azocanyl, wherein the
saturated heterocyclic is optionally monosubstituted with
N(R.sup.A)R.sup.B and optionally substituted with 1 or 2
(CH.sub.2).sub.nR.sup.C; and all other variables are as originally
defined. In an aspect of this sub-class, R.sup.4 is H.
[0145] A second sub-class of the first class (Sub-class C1-S2)
includes the compounds of Formula I and pharmaceutically acceptable
salts thereof, wherein all variables are exactly as defined in
Subclass C1-S1, except that each R.sup.C is independently OH,
O--C.sub.1-4 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, Cl,
Br, F, or CN. In an aspect of this sub-class, R.sup.4 is H.
[0146] A third sub-class of the first class (Sub-class C1-S3)
includes the compounds of Formula I selected from the group
consisting of:
##STR00008##
and pharmaceutically acceptable salts thereof; wherein T is H or
(CH.sub.2).sub.2-3R.sup.C; and R.sup.C and R.sup.2 are each
independently as originally defined or as defined in any of the
preceding embodiments. In one aspect of this sub-class, T is H. In
another aspect of this sub-class R.sup.2 is OSO.sub.3H or
SO.sub.3H. In still another aspect of this sub-class, T is H and
R.sup.2 is OSO.sub.3H or SO.sub.3H. In still another aspect of this
sub-class, T is H and R.sup.2 is OSO.sub.3H. In still another
aspect of this sub-class, each R.sup.C is independently C.sub.1-6
alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl. In a feature of this aspect, R.sup.2 is
OSO.sub.3H.
[0147] A second class of compounds of the present invention (Class
C2) includes compounds of Formula I and pharmaceutically acceptable
salts thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is
HetB; and all other variables are as originally defined. In an
aspect of this class, R.sup.4 is H.
[0148] A first sub-class of the second class (Sub-class C2-S1)
includes the compounds of Formula I and pharmaceutically acceptable
salts thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is
HetB; and HetB is a heteroaromatic ring selected from the group
consisting of pyrrolyl, pyrazolyl, imidazolyl, pyridyl, and
pyrimidinyl, wherein the heteroaromatic ring is optionally
monosubstituted with (CH.sub.2).sub.nN(R.sup.A)R.sup.B and
optionally substituted with 1 or 2 (CH.sub.2).sub.nR.sup.C groups;
and all other variables are as originally defined. In an aspect of
this sub-class, R.sup.4 is H.
[0149] A second sub-class of the second class (Sub-class C2-S2)
includes the compounds of Formula I and pharmaceutically acceptable
salts thereof, wherein all variables are exactly as defined in
Subclass C2-S1, except that each R.sup.C is independently OH,
O--C.sub.1-4 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, Cl,
Br, F, or CN. In an aspect of this sub-class, R.sup.4 is H.
[0150] A third sub-class of the second class (Sub-class C2-S3)
includes the compounds of Formula I and pharmaceutically acceptable
salts thereof, wherein R.sup.1 is C(O)N(R.sup.3)R.sup.4; R.sup.3 is
HetB; and HetB is pyridyl which is optionally monosubstituted with
N(R.sup.A)R.sup.B and optionally substituted with 1 or 2 R.sup.C
groups. In an aspect of this sub-class, R.sup.4 is H.
[0151] A fourth sub-class of the second class (Sub-class C2-S4)
includes the compounds of Formula I and pharmaceutically acceptable
salts thereof, wherein all variables are exactly as defined in
Subclass C2-S3, except that each R.sup.C is independently OH,
O--C.sub.1-4 alkyl, C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, Cl,
Br, F, or CN. In an aspect of this sub-class, R.sup.4 is H. In
still another aspect of this sub-class, each R.sup.C is
independently C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl,
C(.dbd.NH)NH.sub.2, NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl,
pyrrolidinyl, or piperidinyl.
[0152] A fifth sub-class of the second class (Sub-class C2-S5)
includes the compounds of Formula I selected from the group
consisting of:
##STR00009##
and pharmaceutically acceptable salts thereof, wherein R.sup.C is
C.sub.1-6 alkyl, OH, O--C.sub.1-8 alkyl, C(.dbd.NH)NH.sub.2,
NH--C(.dbd.NH)NH.sub.2, halogen, CN, pyridyl, pyrrolidinyl, or
piperidinyl; and R.sup.2, R.sup.A and R.sup.B are each
independently as originally defined. In an aspect of this
sub-class, N(R.sup.A)R.sup.B is NH(C.sub.1-4 alkyl) or N(C.sub.1-4
alkyl).sub.2 and R.sup.C is O--C.sub.1-4 alkyl. In another aspect
of this sub-class, R.sup.2 is OSO.sub.3H or SO.sub.3H. In still
another aspect of this sub-class, R.sup.2 is OSO.sub.3H. In still
another aspect of this sub-class, N(R.sup.A)R.sup.B is NH(C.sub.1-4
alkyl) or N(C.sub.1-4 alkyl).sub.2; R.sup.C is O--C.sub.1-4 alkyl;
and R.sup.2 is OSO.sub.3H or SO.sub.3H. Other aspects of this
sub-class include compounds of formula B1a, B1b, and B1c and their
pharmaceutically acceptable salts, wherein R.sup.C, R.sup.2,
R.sup.A and R.sup.B are each independently as defined in any of the
preceding embodiments; i.e., each unique combination of these
variables constitutes a different aspect.
[0153] A third class of compounds of the present invention (Class
C3) includes compounds of Formula I selected from the group
consisting of:
##STR00010## ##STR00011## ##STR00012##
and pharmaceutically acceptable salts thereof; wherein T is H,
C.sub.1-3 alkyl, pyrrolidin-3-yl, piperidin-4-yl,
(CH.sub.2).sub.2-3--O--C.sub.1-3 alkyl, (CH.sub.2).sub.2-3OH,
(CH.sub.2).sub.2-3F, (CH.sub.2).sub.2-3-piperidinyl,
(CH.sub.2).sub.2-3-pyrrolidinyl; and T' is H, Cl, Br, F, C.sub.1-3
alkyl, O--C.sub.1-3 alkyl, OH, NH.sub.2, N(H)--C.sub.1-3 alkyl, or
N(--C.sub.1-3 alkyl).sub.2; and R.sup.2 is as originally
defined.
[0154] A first sub-class of the third class (Sub-class C3-S1)
includes the compounds of formula (A1) to (A20) and their
pharmaceutically acceptable salts; wherein R.sup.2 is OSO.sub.3H;
and all other variables are as originally defined in Class C1.
[0155] A second sub-class of the third class (Sub-class C3-S2)
includes the compounds of formula (A1) to (A20) and their
pharmaceutically acceptable salts; wherein T is H, CH.sub.3,
pyrrolidin-3-yl, piperidin-4-yl, (CH.sub.2).sub.2-3--OCH.sub.3,
(CH.sub.2).sub.2-3OH, (CH.sub.2).sub.2-3F,
(CH.sub.2).sub.2-3-piperidinyl, (CH.sub.2).sub.2-3-pyrrolidinyl; T'
is H, F, O--C.sub.1-3 alkyl, OH, NH.sub.2, N(H)CH.sub.3,
N(CH.sub.3).sub.2; and; and R.sup.2 is as originally defined. In an
aspect of this sub-class, R.sup.2 is OSO.sub.3H.
[0156] A third sub-class of the third class (Sub-class C3-S3)
includes the compounds of formula (A1) to (A20) and their
pharmaceutically acceptable salts; wherein T is H; T' is H, F,
OCH.sub.3, or OH; and R.sup.2 is OSO.sub.3H.
[0157] A fourth class of compounds of the present invention (Class
C4) includes compounds of Formula I selected from the group
consisting of:
##STR00013## ##STR00014##
and pharmaceutically acceptable salts thereof; wherein V, V', V'',
Y, Y' and Z are each independently selected from the group
consisting of H, CH.sub.3, pyrrolidinyl, piperidinyl, piperazinyl,
morpholinyl, thiomorpholinyl, CH.sub.2-pyrrolidinyl,
CH.sub.2-piperidinyl, CH.sub.2-piperazinyl, CH.sub.2-morpholinyl,
CH.sub.2-thiomorpholinyl, NH.sub.2, N(H)CH.sub.3,
N(CH.sub.3).sub.2, CH.sub.2NH.sub.2, CH.sub.2N(H)CH.sub.3 and
CH.sub.2N(CH.sub.3).sub.2; with the proviso that:
[0158] (i) at least one of V, V' and V'' is H; and
[0159] (ii) at least one of Y and Y' is H.
[0160] A first sub-class of the fourth class (Sub-class C4-S1)
includes the compounds of formula (B1) to (B9) and their
pharmaceutically acceptable salts; wherein at least two of V, V'
and V'' are H; and R.sup.2 is OSO.sub.3H.
[0161] Another embodiment of the present invention is a compound
selected from the group consisting of the title compounds of
Examples 1 to 117 (or, alternatively, Compounds 1 to 117) and
pharmaceutically acceptable salts thereof.
[0162] Another embodiment of the present invention is a compound
selected from the group consisting of the title compounds of
Examples 1 to 13 (i.e., Compounds 1 to 13) and pharmaceutically
acceptable salts thereof.
[0163] Another embodiment of the present invention is a compound
selected from the group consisting of compounds 1, 2, 4 and 6-9 and
pharmaceutically acceptable salts thereof.
[0164] Another embodiment of the present invention is a compound
selected from the group consisting of: [0165]
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide; [0166]
(2S,5R)--N-[4-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide; [0167]
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide; [0168]
(2S,5R)-7-oxo-6-(sulfooxy)-N-(1,2,3,4-tetrahydroisoquinolin-6-yl)-1,6-dia-
zabicyclo[3.2.1]octane-2-carboxamide; [0169]
(2S,5R)-7-oxo-N-(5-piperidin-4-ylpyridin-2-yl)-6-(sulfooxy)-1,6-diazabicy-
clo[3.2.1]octane-2-carboxamide; [0170]
piperidin-4-ylmethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oct-
ane-2-carboxylate; and pharmaceutically acceptable salts
thereof.
[0171] Still another embodiment of the present invention is
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide (i.e., the compound of Example 1 or alternatively
Compound 1) or a pharmaceutically acceptable salt thereof.
[0172] Still another embodiment of the present invention is
(2S,5R)--N-[4-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide (i.e., the compound of Example 9, or
alternatively Compound 9) or a pharmaceutically acceptable salt
thereof.
[0173] Still another embodiment of the present invention is
(2S,5R)-7-oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide (i.e., the compound of Example 14, or
Compound 14) or a pharmaceutically acceptable salt thereof.
[0174] Another embodiment of the present invention is Compound I in
the form of a crystalline monohydrate. The crystalline monohydrate
has the XRPD pattern shown in FIG. 1 and the DSC curve shown in
FIG. 2. The crystalline monohydrate can be prepared as described in
Part A in Example 1D. In one embodiment, the crystalline
monohydrate is characterized by an X-ray powder diffraction pattern
obtained using copper K.sub.a radiation (i.e., the radiation source
is a combination of Cu K.sub..alpha.1 and K.sub..alpha.2 radiation)
which comprises 2.THETA. values (i.e., reflections at 2.THETA.
values) in degrees of about 15.6, 17.4 and 20.4. In this
embodiment, and any analogous embodiments which follow, the term
"about" is understood to modify each of the 2.THETA. values. In
another embodiment, the crystalline monohydrate is characterized by
an X-ray powder diffraction pattern obtained using copper
K.sub..alpha. radiation which comprises 2.THETA. values in degrees
of about 15.6, 17.4, 20.4, 24.0, 26.3 and 29.3. In still another
embodiment the crystalline monohydrate is characterized by an X-ray
powder diffraction pattern obtained using copper K.sub..alpha.
radiation which comprises 2.THETA. values in degrees of about 13.5,
15.5, 15.6, 17.4, 18.7, 19.7, 20.4, 21.7, 22.6, 24.0, 24.3, 25.9,
26.3, 26.6, 27.0, 27.5, 29.3, 30.0, 31.3, 32.4, 32.9, 33.1, 34.0,
34.7, 35.5 and 38.9.
[0175] In still another embodiment, the crystalline monohydrate of
Compound 1 is characterized by the PDF trace derived from its X-ray
diffraction pattern shown in FIG. 1. The PDF trace provides a
fingerprint of the inter-atomic distances that define the
crystalline monohydrate. A PDF trace can be obtained in the manner
described in WO 2005/082050. In one aspect of this embodiment, the
crystalline monohydrate is characterized by the parts of the PDF
trace corresponding to the 2.THETA. values in degrees of about
15.6, 17.4 and 20.4 in the XRPD. In another aspect of this
embodiment, the crystalline monohydrate is characterized by the
parts of the PDF trace corresponding to the 2.THETA. values in
degrees of about 15.6, 17.4, 20.4, 24.0, 26.3 and 29.3 in the XRPD.
In still another aspect of this embodiment, the crystalline
monohydrate is characterized by the parts of the PDF trace
corresponding to the 2.THETA. values in degrees of about 13.5,
15.5, 15.6, 17.4, 18.7, 19.7, 20.4, 21.7, 22.6, 24.0, 24.3, 25.9,
26.3, 26.6, 27.0, 27.5, 29.3, 30.0, 31.3, 32.4, 32.9, 33.1, 34.0,
34.7, 35.5 and 38.9 in the XRPD.
[0176] The term "about", when modifying the quantity (e.g., kg, L,
or equivalents) of a substance or composition, or the value of a
physical property, or the value of a parameter characterizing a
process step (e.g., the temperature at which a process step is
conducted), or the like refers to variation in the numerical
quantity that can occur, for example, through typical measuring,
handling and sampling procedures involved in the preparation,
characterization and/or use of the substance or composition;
through inadvertent error in these procedures; through differences
in the manufacture, source, or purity of the ingredients employed
to make or use the compositions or carry out the procedures; and
the like. In the particular case of the 2.THETA. values in degrees
in an XRPD described herein, the term "about" typically means the
value .+-.0.1.
[0177] Another embodiment of the present invention is a compound of
Formula I, or a pharmaceutically acceptable salt thereof, as
originally defined or as defined in any of the foregoing
embodiments, sub-embodiments, aspects, classes or sub-classes,
wherein the compound or its salt is in a substantially pure form.
As used herein "substantially pure" means suitably at least about
60 wt. %, typically at least about 70 wt. %, preferably at least
about 80 wt. %, more preferably at least about 90 wt. % (e.g., from
about 90 wt. % to about 99 wt. %), even more preferably at least
about 95 wt. % (e.g., from about 95 wt. % to about 99 wt. %, or
from about 98 wt. % to 100 wt. %), and most preferably at least
about 99 wt. % (e.g., 100 wt. %) of a product containing a compound
of Formula I or its salt (e.g., the product isolated from a
reaction mixture affording the compound or salt) consists of the
compound or salt. The level of purity of the compounds and salts
can be determined using a standard method of analysis such as thin
layer chromatography, gel electrophoresis, high performance liquid
chromatography, and/or mass spectrometry. If more than one method
of analysis is employed and the methods provide experimentally
significant differences in the level of purity determined, then the
method providing the highest level of purity governs. A compound or
salt of 100% purity is one which is free of detectable impurities
as determined by a standard method of analysis. With respect to a
compound of the invention which has one or more asymmetric centers
and can occur as mixtures of stereoisomers, a substantially pure
compound can be either a substantially pure mixture of the
stereoisomers or a substantially pure individual diastereomer or
enantiomer.
[0178] Other embodiments of the present invention include the
following:
[0179] (a) A pharmaceutical composition comprising an effective
amount of a compound of Formula I as defined above, or a
pharmaceutically acceptable salt thereof, and a pharmaceutically
acceptable carrier.
[0180] (b) The pharmaceutical composition of (a), further
comprising an effective amount of a .beta.-lactam antibiotic.
[0181] (c) The pharmaceutical composition of (b), wherein the
beta-lactam antibiotic is selected from the group consisting of
imipenem, ertapenem, meropenem, doripenem, biapenem, panipenem,
ticarcillin, ampicillin, amoxicillin, carbenicillin, piperacillin,
azlocillin, mezlocillin, ticarcillin, cefoperazone, cefotaxime,
ceftriaxone, and ceftazidime.
[0182] (d) The pharmaceutical composition of (b), wherein the
.beta.-lactam antibiotic is imipenem.
[0183] (e) The pharmaceutical composition of (b), wherein the
.beta.-lactam antibiotic is ceftazidime.
[0184] (f) The pharmaceutical composition of (b), wherein the
.beta.-lactam antibiotic is piperacillin.
[0185] (g) The pharmaceutical composition of (a), further
comprising effective amounts of a .beta.-lactam antibiotic and a
DHP inhibitor.
[0186] (h) The pharmaceutical composition of (g), wherein the
beta-lactam antibiotic is imipenem, and the DHP inhibitor is
cilastatin or a pharmaceutically acceptable salt thereof.
[0187] (i) A combination of effective amounts of a compound of
Formula I as defined above, or a pharmaceutically acceptable salt
thereof, and a .beta.-lactam antibiotic.
[0188] (j) The combination of (i), wherein the beta-lactam
antibiotic is selected from the group consisting of imipenem,
ertapenem, meropenem, doripenem, biapenem, panipenem, ticarcillin,
ampicillin, amoxicillin, carbenicillin, piperacillin, azlocillin,
mezlocillin, ticarcillin, cefoperazone, cefotaxime, ceftriaxone,
and ceftazidime.
[0189] (k) The combination of (i), wherein the .beta.-lactam
antibiotic is imipenem.
[0190] (l) The combination of (i), wherein the .beta.-lactam
antibiotic is ceftazidime.
[0191] (m) The combination of (i), wherein the .beta.-lactam
antibiotic is piperacillin.
[0192] (n) A combination of effective amounts of a compound of
Formula I as defined above, or a pharmaceutically acceptable salt
thereof, a .beta.-lactam antibiotic and a DHP inhibitor.
[0193] (o) The combination of (n), wherein the beta-lactam
antibiotic is imipenem, and the DHP inhibitor is cilastatin or a
pharmaceutically acceptable salt thereof.
[0194] (p) A method for treating a bacterial infection which
comprises administering to a subject in need of such treatment a
therapeutically effective amount of a compound of Formula I, or a
pharmaceutically acceptable salt thereof, optionally in combination
with an effective amount of a beta-lactam antibiotic.
[0195] (q) A method for treating a bacterial infection which
comprises administering to a subject in need of such treatment a
therapeutically effective amount of a compound of Formula I, or a
pharmaceutically acceptable salt thereof, in combination with
effective amounts of a beta-lactam antibiotic and a DHP
inhibitor.
[0196] (r) A method for treating a bacterial infection which
comprises administering to a subject in need of such treatment a
therapeutically effective amount of the composition of (a), (b),
(c), (d), (e), (f), (g) and (h).
[0197] (s) A method for treating a bacterial infection which
comprises administering to a subject in need of such treatment a
therapeutically effective amount of the combination (i), (j), (k),
(l), (m), (n) and (o).
[0198] (t) The method of treating a bacterial infection as set
forth in (p), (q), (r), or (s), wherein the bacterial infection is
due to Pseudomonas spp. or Klebsiella spp.
[0199] The present invention also includes a compound of Formula I,
or a pharmaceutically acceptable salt thereof, (i) for use in, (ii)
for use as a medicament for, or (iii) for use in the preparation
(or manufacture) of a medicament for treating bacterial infection.
In these uses, the compounds of the present invention can
optionally be employed in combination with one or more
.beta.-lactam antibiotics and/or one or more DHP inhibitors.
[0200] Additional embodiments of the invention include the
pharmaceutical compositions, combinations and methods set forth in
(a)-(t) above and the uses set forth in the preceding paragraph,
wherein the compound of the present invention employed therein is a
compound of one of the embodiments, sub-embodiments, classes or
sub-classes described above. The compound may optionally be used in
the form of a pharmaceutically acceptable salt in these
embodiments.
[0201] Additional embodiments of the present invention include each
of the pharmaceutical compositions, combinations, methods and uses
set forth in the preceding paragraphs, wherein the compound of the
present invention or its salt employed therein is substantially
pure. With respect to a pharmaceutical composition comprising a
compound of Formula I or its salt and a pharmaceutically acceptable
carrier and optionally one or more excipients, it is understood
that the term "substantially pure" is in reference to a compound of
Formula I or its salt per se; i.e., the purity of the active
ingredient in the composition. As used herein, the term "alkyl"
refers to a monovalent straight or branched chain, saturated
aliphatic hydrocarbon radical having a number of carbon atoms in
the specified range. Thus, for example, "C.sub.1-8 alkyl" (or
"C.sub.1-C.sub.8 alkyl") refers to any of the octyl, heptyl, hexyl
and pentyl alkyl isomers as well as n-, iso-, sec- and t-butyl, n-
and iso-propyl, ethyl and methyl. As another example, "C.sub.1-6
alkyl" (or "C.sub.1-C.sub.6 alkyl") refers to any of the hexyl and
pentyl alkyl isomers as well as n-, iso-, sec- and t-butyl, n- and
iso-propyl, ethyl and methyl. As another example, "C.sub.1-4 alkyl"
refers to n-, iso-, sec- and t-butyl, n- and isopropyl, ethyl and
methyl.
[0202] The term "haloalkyl" refers to an alkyl group as defined
above in which one or more of the hydrogen atoms has been replaced
with a halogen (i.e., F, Cl, Br and/or I). Thus, for example,
"C.sub.1-6 haloalkyl" (or "C.sub.1-C.sub.6 haloalkyl") refers to a
C.sub.1 to C.sub.6 linear or branched alkyl group as defined above
with one or more halogen substituents. The term "fluoroalkyl" has
an analogous meaning except that the halogen substituents are
restricted to fluoro. Suitable fluoroalkyls include the series
(CH.sub.2).sub.0-4CF.sub.3 (i.e., trifluoromethyl,
2,2,2-trifluoroethyl, 3,3,3-trifluoro-n-propyl, etc.).
[0203] The term "halogen" (or "halo") refers to fluorine, chlorine,
bromine and iodine (alternatively referred to as fluoro, chloro,
bromo, and iodo).
[0204] The term "cycloalkyl" refers to any monovalent monocyclic
ring of an alkane having a number of carbon atoms in the specified
range. Thus, for example, "C.sub.4-9 cycloalkyl" (or
"C.sub.4-C.sub.9 cycloalkyl") refers to cyclobutyl, cyclopentyl,
cyclohexyl, cycloheptyl, cyclooctyl, and cyclononyl, and "C.sub.4-7
cycloalkyl" refers to cyclobutyl, cyclopentyl, cyclohexyl, and
cycloheptyl.
[0205] The term "C(O)" refers to carbonyl. The terms "S(O).sub.2"
and "SO.sub.2" each refer to sulfonyl. The term "S(O)" refers to
sulfinyl.
[0206] The symbol "*" at the end of a bond refers to the point of
attachment of a functional group or other chemical moiety to the
rest of the molecule of which it is a part.
[0207] Unless expressly limited, the term "substituted" refers to
single and multiple substitution by a named substituent to the
extent such single and multiple substitution (including multiple
substitution at the same site) is chemically allowed. Unless
expressly stated to the contrary, substitution by a named
substituent is permitted on any atom in a ring (e.g., cycloalkyl,
phenyl, a heteroaromatic ring, or a saturated heterocyclic ring)
provided such ring substitution is chemically allowed and results
in a stable compound. It is understood, however, that the degree of
substitution can be qualified. For example, the expression
"optionally substituted with a total of from 1 to 4 substituents
selected from zero to 2 N(R.sup.A)R.sup.B and zero to 2 R.sup.C"
means that there can optionally be 4 substituents in total with a
maximum of 2 N(R.sup.A)R.sup.B substituents and a maximum of 2
R.sup.C groups. As another example, the expression "AryA is neither
unsubstituted phenyl nor phenyl substituted with 1 or 2
N(R.sup.A)R.sup.B" means AryA is not phenyl, phenyl
mono-substituted with N(R.sup.A)R.sup.B, or phenyl di-substituted
with N(R.sup.A)R.sup.B, and otherwise AryA is as elsewhere
defined.
[0208] HetA is defined herein to be a 4- to 9-membered saturated or
mono-unsaturated heterocyclic ring containing from 1 to 3
heteroatoms independently selected from N, O and S, wherein the
ring is optionally fused with a C.sub.3-7 cycloalkyl. Saturated
heterocyclic rings suitable for use as HetA include, for example,
azetidinyl, piperidinyl, morpholinyl, thiomorpholinyl,
thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl,
pyrrolidinyl, imidazolidinyl, piperazinyl, tetrahydrofuranyl,
tetrahydrothienyl, pyrazolidinyl, hexahydropyrimidinyl,
thiazinanyl, thiazepanyl, azepanyl, diazepanyl, azocanyl
(=octahydroazocinyl), azonanyl (=octahydro-1H-azoninyl),
tetrahydropyranyl, tetrahydrothiopyranyl, and dioxanyl. Suitable
mono-unsaturated heterocyclic rings include, for example, rings
corresponding to the saturated rings set forth in the preceding
sentence except that they contain a double bond (e.g., a
carbon-carbon double bond). Saturated heterocyclic rings fused with
a cycloalkyl suitable for use as HetA include, for example,
##STR00015##
[0209] HetB is defined herein to be an optionally substituted
heteroaromatic ring containing from 1 to 4 heteroatoms selected
from 1 to 3 N atoms, zero or 1 O atom, and zero or 1 S atom,
wherein the heteroaromatic ring is optionally fused with a 5- to
7-membered, saturated heterocyclic ring containing 1 or 2
heteroatoms independently selected from N, O and S, wherein any
ring S is optionally oxidized to S(O) or S(O).sub.2 and either 1 or
2 non-fused ring carbons are optionally oxidized to C(O).
Heteroaromatic rings suitable for use as HetB include, for example,
pyridyl (also referred to as pyridinyl), pyrrolyl, pyrazinyl,
pyrimidinyl, pyridazinyl, triazinyl, imidazolyl, pyrazolyl,
triazolyl, oxazolyl, isooxazolyl, oxadiazolyl, oxatriazolyl,
thiazolyl, isothiazolyl, and thiadiazolyl. Fused rings suitable for
use as HetB include, for example,
##STR00016##
[0210] Unless expressly stated to the contrary in a particular
context, any of the various cyclic rings and ring systems described
herein may be attached to the rest of the compound at any ring atom
(i.e., any carbon atom or any heteroatom) provided that a stable
compound results.
[0211] Unless expressly stated to the contrary, all ranges cited
herein are inclusive. For example, a heteroaromatic ring described
as containing from "1 to 4 heteroatoms" means the ring can contain
1, 2, 3 or 4 heteroatoms. It is also to be understood that any
range cited herein includes within its scope all of the sub-ranges
within that range. Thus, for example, a heterocyclic ring described
as containing from "1 to 4 heteroatoms" is intended to include as
aspects thereof, heterocyclic rings containing 2 to 4 heteroatoms,
3 or 4 heteroatoms, 1 to 3 heteroatoms, 2 or 3 heteroatoms, 1 or 2
heteroatoms, 1 heteroatom, 2 heteroatoms, 3 heteroatoms, and 4
heteroatoms.
[0212] When any variable (e.g., R.sup.A or R.sup.B) occurs more
than one time in any constituent or in Formula I or in any other
formula depicting and describing compounds of the present
invention, its definition on each occurrence is independent of its
definition at every other occurrence. Also, combinations of
substituents and/or variables are permissible only if such
combinations result in stable compounds.
[0213] A "stable" compound is a compound which can be prepared and
isolated and whose structure and properties remain or can be caused
to remain essentially unchanged for a period of time sufficient to
allow use of the compound for the purposes described herein (e.g.,
therapeutic administration to a subject). The compounds of the
present invention are limited to stable compounds embraced by
Formula I.
[0214] The compounds of the present invention have at least two
asymmetric centers and can have one or more additional centers as a
result of the presence of certain substituents and/or substituent
patterns. Accordingly, compounds of the invention can occur as
mixtures of stereoisomers, or as individual diastereomers, or
enantiomers. All isomeric forms of these compounds, whether
individually or in mixtures, are within the scope of the present
invention.
[0215] The term "compound" refers to the free compound and, to the
extent they are stable, any hydrate or solvate thereof. A hydrate
is the compound complexed with water, and a solvate is the compound
complexed with an organic solvent.
[0216] As indicated above, the compounds of the present invention
can be employed in the form of pharmaceutically acceptable salts.
The term "pharmaceutically acceptable salt" refers to a salt which
possesses the effectiveness of the parent compound and which is not
biologically or otherwise undesirable (e.g., is neither toxic nor
otherwise deleterious to the recipient thereof). A suitable
pharmaceutically acceptable salt is a salt formed by treating the
compound of the invention (e.g., a compound of Formula I) with one
molar equivalent of a mild base (e.g., sodium carbonate, sodium
bicarbonate, potassium bicarbonate, or sodium acetate). In this
case, M is a cation, such as Na.sup.+ in the event of treatment
with a sodium base.
[0217] When M is H (e.g., R.sup.2 is OSO.sub.3H) and the compound
of the invention contains an internal base which is capable of
being protonated (e.g., R.sup.1 contains a basic nitrogen), it is
understood that the compound might exist in a form in which the
internal base is completely protonated by M=H such that R.sup.2
possesses a negative charge (e.g., R.sup.2.dbd.OSO.sub.3.sub.-) and
the internal base has a positive charge, or is partially protonated
such that R.sup.2 possesses a partial negative charge, or is not
protonated. Similarly, when M is H and the compound of the
invention contains two or more internal bases which are capable of
being protonated (e.g., R.sup.1 contains two or more basic
nitrogens), it is understood that the compound might exist in a
form in which one or another of the internal bases is completely
protonated by M=H or that two or more of the internal bases are
each sufficiently protonated such that R.sup.2 possesses a negative
charge, or that one or more of the bases is partially protonated
such that R.sup.2 possesses a partial negative charge, or that none
of the bases is protonated. The present invention includes all such
forms of the compound. While these compounds can be in the form of
an internal salt (i.e., a zwitterion), they are considered herein
compounds of the invention, not pharmaceutically acceptable salts
thereof.
[0218] On the other hand, for a compound of the invention which
contains an internal base (e.g., R.sup.1 contains a basic
nitrogen), a pharmaceutically acceptable salt is a salt formed by
treatment of the compound with a suitable amount of an acid (e.g.,
hydrochloric acid, trifluoroacetic acid, methanesulfonic acid, or
the like) such that the internal base is protonated by the acid
with the positive charge of the protonated base balanced by a
negative counterion (e.g., chloride, trifluoride, methanesulfonate,
or the like). For compounds of the invention containing two
internal bases (e.g., R.sup.1 contains two basic nitrogens),
another pharmaceutically acceptable salt is a salt formed by
treatment of the compound with a suitable amount of acid such that
one of the internal bases is protonated by the sulfonic acid group
present in the molecule (i.e., R.sup.2 has a negative charge) and
the other is protonated by the acid with the positive charge of the
protonated base balanced by a suitable negative counterion. Still
another pharmaceutically acceptable salt for compounds of the
invention containing two internal bases can be obtained by treating
the compound with sufficient acid (e.g., sulfuric acid, HCl,
methanesolufonic acid, or TFA) such that the sulfonic acid group
present in the molecule remains protonated (i.e., M=H) and the
internal bases are protonated and have associated therewith a
suitable negative counterion (e.g., sulfonate). As is clear from
the foregoing, the precise nature and type of pharmaceutically
acceptable salt which can be obtained will depend upon the nature
of the specific compound being treated (e.g., the presence or
absence of basic nitrogens in R.sup.1) and the treatment conditions
employed; e.g., it will depend upon the choice and amount of the
acid or base with which the compound is treated, the pH of the
treating media, the amount and choice of buffer (if any), and the
like. It is understood that the present invention encompasses all
types and forms of pharmaceutically acceptable salts of the
compounds of the present invention.
[0219] As set forth above, the present invention includes
pharmaceutical compositions comprising a compound of Formula I of
the present invention, optionally one or more other active
components (e.g., a .beta.-lactam antibiotic), and a
pharmaceutically acceptable carrier. The characteristics of the
carrier will depend on the route of administration. By
"pharmaceutically acceptable" is meant that the ingredients of the
pharmaceutical composition must be compatible with each other, do
not interfere with the effectiveness of the active ingredient(s),
and are not deleterious (e.g., toxic) to the recipient thereof.
Thus, compositions according to the invention may, in addition to
the inhibitor, contain diluents, fillers, salts, buffers,
stabilizers, solubilizers, and other materials well known in the
art.
[0220] Also as set forth above, the present invention includes a
method for treating a bacterial infection which comprises
administering to a subject in need of such treatment a
therapeutically effective amount of a compound of Formula I, or a
pharmaceutically acceptable salt thereof, optionally in combination
with a beta-lactam antibiotic and/or a DHP inhibitor. The term
"subject" (or, alternatively, "patient") as used herein refers to
an animal, preferably a mammal, most preferably a human, who has
been the object of treatment, observation or experiment. The term
"administration" and variants thereof (e.g., "administering" a
compound) in reference to a compound of Formula I mean providing
the compound, or a pharmaceutically acceptable salt thereof, to the
individual in need of treatment. When a compound or a salt thereof
is provided in combination with one or more other active agents
(e.g., a carbapenem antibiotic or a DHP inhibitor or both),
"administration" and its variants are each understood to include
provision of the compound or its salt and the other agents at the
same time or at different times. When the agents of a combination
are administered at the same time, they can be administered
together in a single composition or they can be administered
separately. It is understood that a "combination" of active agents
can be a single composition containing all of the active agents or
multiple compositions each containing one or more of the active
agents. In the case of two active agents a combination can be
either a single composition comprising both agents or two separate
compositions each comprising one of the agents; in the case of
three active agents a combination can be either a single
composition comprising all three agents, three separate
compositions each comprising one of the agents, or two compositions
one of which comprises two of the agents and the other comprises
the third agent; and so forth.
[0221] The compositions and combinations of the present invention
are suitably administered in effective amounts. The term "effective
amount" as used herein means that amount of active compound or
pharmaceutical agent that elicits the biological or medicinal
response in a tissue, system, animal or human that is being sought
by a researcher, veterinarian, medical doctor or other clinician.
In one embodiment, the effective amount is a "therapeutically
effective amount" for the alleviation of the symptoms of the
disease or condition being treated (e.g., the healing of conditions
associated with bacterial infection, and/or bacterial drug
resistance). In another embodiment, the effective amount is a
"prophylactically effective amount" for prophylaxis of the symptoms
of the disease or condition being prevented. The term also includes
herein the amount of active compound sufficient to inhibit
.beta.-lactamase and thereby elicit the response being sought
(i.e., an "inhibition effective amount"). When the active compound
(i.e., active ingredient) is administered as the salt, references
to the amount of active ingredient are to the free acid or free
base form of the compound.
[0222] The administration of a composition of the present invention
is suitably parenteral, oral, sublingual, transdermal, topical,
intranasal, intratracheal, or intrarectal, wherein the composition
is suitably formulated for administration by the selected route
using formulation methods well known in the art, including, for
example, the methods for preparing and administering formulations
described in chapters 39, 41, 42, 44 and 45 in Remington--The
Science and Practice of Pharmacy, 21.sup.st edition, 2006. In one
embodiment, compounds of the invention are administered
intravenously in a hospital setting. In another embodiment,
administration is oral in the form of a tablet or capsule or the
like. When administered systemically, a therapeutic composition is
suitably administered at a sufficient dosage to attain a blood
level of inhibitor of at least about 1 microgram/mL, preferably
about 10 micrograms/mL, and more preferably about 25 micrograms/mL.
For localized administration, much lower concentrations than this
may be effective, and much higher concentrations may be
tolerated.
[0223] Intravenous administration of a compound of the invention
can be conducted by reconstituting a powdered form of the compound
with an acceptable solvent. Suitable solvents include, for example,
saline solutions (e.g., 0.9% Sodium Chloride Injection) and sterile
water (e.g., Sterile Water for Injection, Bacteriostatic Water for
Injection with methylparaben and propylparaben, or Bacteriostatic
Water for Injection with 0.9% benzyl alcohol). The powdered form of
the compound can be obtained by gamma-irradiation of the compound
or by lyophilization of a solution of the compound, after which the
powder can be stored (e.g., in a sealed vial) at or below room
temperature until it is reconstituted. The concentration of the
compound in the reconstituted IV solution can be, for example, in a
range of from about 0.1 mg/mL to about 20 mg/mL.
[0224] The present invention also includes a method for inhibiting
bacterial growth which comprises administering to a bacterial cell
culture, or to a bacterially infected cell culture, tissue, or
organism, an inhibition effective amount of a compound of Formula
I. Additional embodiments of the invention include the bacterial
growth inhibiting method just described, wherein the compound of
the present invention employed therein is a compound of one of the
embodiments, sub-embodiments or classes described above. The
compound may optionally be used in the form of a pharmaceutically
acceptable salt in these embodiments. The method can involve
administration of a compound of Formula I to an experimental cell
culture in vitro to prevent the growth of .beta.-lactam resistant
bacteria. The method can alternatively involve administration of a
compound of Formula I to an animal, including a human, to prevent
the growth of .beta.-lactam resistant bacteria in vivo. In these
cases the compound of Formula I is typically co-administered with a
.beta.-lactam antibiotic.
[0225] Compounds of the invention can be employed for the
treatment, prophylaxis or inhibition of bacterial growth or
infections due to bacteria that are resistant to .beta.-lactam
antibiotics. More particularly, the bacteria can be
.beta.-lactamase positive strains that are highly resistant to
.beta.-lactam antibiotics. The terms "slightly resistant" and
"highly resistant" are well-understood by those of ordinary skill
in the art (see, e.g., Payne et al., Antimicrobial Agents and
Chemotherapy 38:767-772 (1994); Hanaki et al., Antimicrobial Agents
and Chemotherapy 30:11.20-11.26 (1995)). For the purposes of this
invention, bacterial strains which are highly resistant to imipenem
are those against which the MIC of imipenem is >16 .mu.g/mL, and
bacterial strains which are slightly resistant to imipenem are
those against which the MIC of imipenem is >4 .mu.g/mL.
[0226] Compounds of the invention can be used in combination with
antibiotic agents for the treatment of infections caused by Class
C-.beta.-lactamase producing strains, in addition to those
infections which are subsumed within the antibacterial spectrum of
the antibiotic agent. Examples of class C-.beta.-lactamase
producing bacteria are Pseudomonas aeruginosa, Enterobacter
cloacae, Klebsiella pneumoniae, Escherichia coli and Acinetobacter
baumannii.
[0227] It is generally advantageous to use a compound of Formula I
in admixture or conjunction with a carbapenem, penicillin,
cephalosporin, or other .beta.-lactam antibiotic, or a prodrug
thereof. It is advantageous to use a compound of Formula I in
combination with one or more .beta.-lactam antibiotics because of
the class C .beta.-lactamase inhibitory properties of the
compounds. As already noted, the compound of Formula I and the
.beta.-lactam antibiotic can be administered separately (at the
same time or as different times) or in the form of a single
composition containing both active ingredients.
[0228] Carbapenems, penicillins, cephalosporins and other
.beta.-lactam antibiotics suitable for use in the present invention
include both those known to show instability to or to be otherwise
susceptible to class C-.beta.-lactamases and also known to have a
degree of resistance to class C .beta.-lactamase.
[0229] When the compounds of Formula I are combined with a
carbapenem antibiotic, a dehydropeptidase (DHP) inhibitor can also
be combined. Many carbapenems are susceptible to attack by a renal
enzyme known as DHP. This attack or degradation may reduce the
efficacy of the carbapenem antibacterial agent Inhibitors of DHP
and their use with carbapenems are disclosed in, e.g., U.S. Pat.
No. 4,539,208, U.S. Pat. No. 4,616,038, U.S. Pat. No. 4,880,793 and
U.S. Pat. No. 5,071,843. A preferred DHP inhibitor is
7-(L-2-amino-2-carboxyethylthio)-2-(2,2-dimethylcyclopropanecarboxamide)--
2-heptenoic acid or a pharmaceutically acceptable salt thereof.
[0230] Carbapenems suitable for co-administration with compounds of
the present invention include imipenem, meropenem, biapenem,
(4R,5S,6S)-3-[3S,5S)-5-(3-carboxyphenyl-carbamoyl)pyrrolidin-3-ylthio]-6--
(1R)-1-hydroxyethyl]-4-methyl-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carbox-
ylic acid,
(1S,5R,6S)-2-(4-(2-(((carbamoylmethyl)-1,4-diazoniabicyclo[2.2.-
2]oct-1-yl)-ethyl(1,8-naphthosultam)methyl)-6-[1(R)-hydroxyethyl]-1-methyl-
carbapen-2-em-3-carboxylate chloride, BMS181139
([4R-[4alpha,5beta,6beta(R*)]]-4-[2-[(aminoiminomethyl)amino]ethyl]-3-[(2-
-cyanoethyl)thio]-6-(1-hydroxyethyl)-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-
-carboxylic acid), BO2727 ([4R-3[3S*,5S*(R*)],
4alpha,5beta,6beta(R*)]]-6-(1-hydroxyethyl)-3-[[5-[1-hydroxy-3-(methylami-
no)propyl]-3-pyrrolidinyl]thio]-4-methyl-7-oxo-1-azabicyclo[3.2.0]hept-2-e-
ne-2-carboxylic acid monohydrochloride), E1010
((1R,5S,6S)-6-[1(R)-hydroxymethyl]-2-[2(S)-[1(R)-hydroxy-1-[pyrrolidin-3(-
R)-yl]methyl]pyrrolidin-4(S)-ylsulfanyl]-1-methyl-1-carba-2-penem-3-carbox-
ylic acid hydrochloride) and S4661
((1R,5S,6S)-2-[(3S,5S)-5-(sulfamoylaminomethyl)
pyrrolidin-3-yl]thio-6-[(1R)-1-hydroxyethyl]-1-methylcarbapen-2-em-3-carb-
oxylic acid),
(1S,5R,6S)-1-methyl-2-{7-[4-(aminocarbonylmethyl)-1,4-diazoniabicyclo(2.2-
.2)octan-1yl]-methyl-fluoren-9-on-3-yl}-6-(1R-hydroxyethyl)-carbapen-2-em--
3 carboxylate chloride.
[0231] Penicillins suitable for co-administration with compounds of
the present invention include benzylpenicillin,
phenoxymethylpenicillin, carbenicillin, azidocillin, propicillin,
ampicillin, amoxicillin, epicillin, ticarcillin, cyclacillin,
pirbenicillin, azlocillin, mezlocillin, sulbenicillin,
piperacillin, and other known penicillins. The penicillins may be
used in the form of pro-drugs thereof; for example as in vivo
hydrolysable esters, for example the acetoxymethyl,
pivaloyloxymethyl, .alpha.-ethoxycarbonyloxy-ethyl and phthalidyl
esters of ampicillin, benzylpenicillin and amoxicillin; as aldehyde
or ketone adducts of penicillins containing a
6-.alpha.-aminoacetamido side chain (for example hetacillin,
metampicillin and analogous derivatives of amoxicillin); and as
esters of carbenicillin and ticarcillin, for example the phenyl and
indanyl .alpha.-esters.
[0232] Cephalosporins suitable for co-administration with compound
of the present invention include cefatrizine, cephaloridine,
cephalothin, cefazolin, cephalexin, cephacetrile, cephapirin,
cephamandole nafate, cephradine, 4-hydroxycephalexin,
cephaloglycin, cefoperazone, cefsulodin, ceftazidime, cefuroxime,
cefmetazole, cefotaxime, ceftriaxone, and other known
cephalosporins, all of which may be used in the form of pro-drugs
thereof.
[0233] .beta.-Lactam antibiotics other than penicillins and
cephalosporins that may be co-administered with compounds of the
present invention include aztreonam, latamoxef (Moxalactam-trade
mark), and other known .beta.-lactam antibiotics such as
carbapenems like imipenem, meropenem or
(4R,5S,6S)-3-[(3S,5S)-5-(3-carboxyphenylcarbamoyl)pyrrolidin-3-ylthio]-6--
(1R)-1-hydroxyethyl]-4-methyl-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carbox-
ylic acid, all of which may be used in the form of pro-drugs
thereof.
[0234] In one embodiment, the antibiotic co-administered with a
compound of the present invention is selected from the group
consisting of imipenem, meropenem and
(4R,5S,6S)-3-[(3S,5S)-5-(3-carboxyphenylcarbamoyl)pyrrolidin-3-ylthio]-6--
(1R)-1-hydroxyethyl]-4-methyl-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carbox-
ylic acid.
[0235] In another embodiment, the antibiotic co-administered with a
compound of the present invention is selected from the group of
penicillins consisting of ampicillin, amoxicillin, carbenicillin,
piperacillin, azlocillin, mezlocillin, and ticarcillin. Such
penicillins can optionally be used in the form of their
pharmaceutically acceptable salts, for example their sodium salts.
Ampicillin or amoxicillin can alternatively be employed in the form
of fine particles of the zwitterionic form (generally as ampicillin
trihydrate or amoxicillin trihydrate) for use in an injectable or
infusable suspension. In an aspect of this embodiment, the
penicillin co-administered with a compound of the present invention
is amoxicillin, optionally in the form of its sodium salt or the
trihydrate.
[0236] In another embodiment, the antibiotic co-administered with a
compound of the present invention is selected from the group of
cephalosporins consisting of cefotaxime, ceftriaxone and
ceftazidime, which are optionally used in the form of their
pharmaceutically acceptable salts, for example their sodium
salts.
[0237] When co-administered with a .beta.-lactam antibiotic, the
combination of the compound of the invention and the antibiotic can
provide a a synergistic effect. The terms "synergistic effect" and
"synergy" indicate that the effect produced when two or more drugs
are co-administered is greater than would be predicted based on the
effect produced when the compounds are administered individually.
While not wishing to be bound by theory, it is believed that the
compounds of the present invention are .beta.-lactamase inhibitors
that act to prevent degradation of .beta.-lactam antibiotics,
thereby enhancing their efficacy and producing a synergistic
effect.
[0238] Abbreviations employed herein include the following:
acac=acetylacetonate; AIBN=2,2-azobisisobutyronitrile;
BLI=beta-lactamase inhibitor; Bn=benzyl; BOC (or
Boc)=t-butyloxycarbonyl;
BOC-ON=2-(tert-butoxycarbonyloxyamino)-2-phenyl acetonitrile;
BOC-OSN=N-tert-butoxycarbonyloxy)succinimide;
BOP=benzotriazol-1-yloxy)tris(dimethylamino)phosphonium
hexafluorophosphate; BSA=bovine serum albumin; CBZ (or
Cbz)=carbobenzoxy (alternatively, benzyloxycarbonyl);
COD=cyclooctadieneyl; DBU=1,8-diazabicyclo[5.4.0]undec-7-ene;
DCC=dicyclohexyl carbodiimide; DCE=1,2-dichloroethane;
DCM=dichloromethane; DIPEA=diisopropylethylamine (or Hunig's base);
DMAC=N,N-dimethylacetamide; DMAP=4-dimethylaminopyridine
N,N-dimethylaminopyridine; DME=1,2-dimethoxyethane;
DMF=N,N-dimethylformamide; DMSO=dimethyl sulfoxide;
EDC=1-ethyl-3-(3-dimethylaminopropyl) carbodiimide;
DSC=differential scanning calorimetry; Et=ethyl; EtOAc=ethyl
acetate; HMDS=hexamethyldisilazide; HOBT=1-hydroxy benzotriazole;
HOPO=2-hydroxypyridine-N-oxide; HPLC=high-performance liquid
chromatography; IPA=isopropyl alcohol; IPAc=isopropyl acetate;
i-Pr=isopropyl; LC/MS=liquid chromatography/mass spectrometry;
Me=methyl; MHBII=Mueller Hinton Broth type II; MIC=minimum
inhibitory concentration; MSA=methanesulfonic acid;
NMP=N-methylpyrrolidinone; PG=protective group; Ph=phenyl;
TEA=triethylamine; TFA=trifluoroacetic acid;
TFE=2,2,2-trifluoroethaonol; THF=tetrahydrofuran; TLC=thin layer
chromatography; TSB=trypticase soy broth; TsOH=p-toluenesulfonic
acid; XRPD=X-ray powder diffraction.
[0239] The compounds of the present invention can be readily
prepared according to the following reaction schemes and examples,
or modifications thereof, using readily available starting
materials, reagents and conventional synthesis procedures
including, for example, procedures described in U.S. Pat. No.
7,112,592. In these reactions, it is also possible to make use of
variants which are themselves known to those of ordinary skill in
this art, but are not mentioned in greater detail. Furthermore,
other methods for preparing compounds of the invention will be
readily apparent to the person of ordinary skill in the art in
light of the following reaction schemes and examples. Unless
otherwise indicated, all variables are as defined above.
[0240] Carboxamide compounds of the present invention in which a is
a single bond and X is (CH.sub.2).sub.1-3 can be prepared as
depicted in Scheme 1:
##STR00017##
[0241] The bicyclic intermediate A can be obtained as described in
U.S. Pat. No. 7,112,592 or via routine modifications thereof. The
side chain can be attached by the reaction of acid A with amine B
(wherein if necessary the amine incorporates a protective group)
under standard amide formation conditions known in the art. For
example, a solution of acid A and amine B (1-2 molar equivalents)
in solvent (e.g., a haloalkane such as dry dichloromethane or
chloroform) can be stirred at room temperature while sequentially
adding triethylamine (1-2 equivalents), HOBT (1-2 equivalents), and
EDC (1-2 equivalents) at room temperature under nitrogen. The
resulting reaction mixture can then be stirred at room temperature
until the reaction is complete (e.g., in about 8 to 24 hours), and
then the reaction mixture can be concentrated under vacuum and the
residue purified using column chromatography on silica gel or HPLC
to afford the amide C. Deprotection of the benzylic ether
protecting group to afford intermediate hydroxylactam D can be
accomplished by hydrogenation or, in some cases, by acid-catalyzed
hydrolysis. For example, palladium on carbon (0.05-0.5 eq) can be
added to a solution of the benzylic ether in a suitable solvent
(e.g., an alcohol such methanol or ethanol, an alkyl acetate such
as EtOAc, or an ether such as THF) and the resulting mixture
stirred under hydrogen (1-3 atmospheres) until reaction is complete
(e.g., about 1 to 24 hours) as determined by a suitable monitoring
technique such as TLC or HPLC. Upon completion, hydroxy lactam D
can be isolated using conventional techniques. For example, the
reaction mixture can be filtered and the filtrate concentrated to
provide a crude hydroxylactam D which in many cases can be employed
directly in the next step without further purification. If further
purification is necessary or desired, the crude hydroxylactam D can
be purified by column chromatography on silica gel or by HPLC to
afford pure hydroxylactam D. Sulfation of intermediate D to afford
the sulfate E can be accomplished using a sulfating reagent in an
appropriate solvent. Thus, sulfur trioxide pyridine complex (2-10
equivalents) can be added to a solution of hydroxylactam D in an
aprotic solvent (e.g., a tertiary amide such as pyridine, DMF, or
DMAC) at room temperature. The resulting mixture can be stirred at
room temperature until the reaction is complete (e.g., about 4 to
24 hours) as monitored by HPLC or LC/MS. Additional sulfur trioxide
pyridine complex can be added as necessary to drive the reaction to
completion. A purified reaction product can be obtained using
conventional techniques such as by filtering the reaction mixture,
concentrating the filtrate in vacuo, suspending the concentrate in
a saturated aqueous potassium dihydrogenphosphate solution, washing
the aqueous solution with a suitable organic solvent (e.g., EtOAc),
adding excess tetrabutylammonium hydrogen sulfate to the aqueous
layer, extracting the mixture with organic solvent(e.g., EtOAc
4.times.), combining the organic layers, drying the combined
organics over sodium sulfate, and concentrating in vacuo to afford
the tetrabutylammonium salt of intermediate E. In cases where there
is no protective group in the side chain, the product of the
sulfation reaction is a compound of Formula I of the present
invention. When a protective group is incorporated in the amide
side chain (e.g., a benzyl amine or ether, BOC amine, or a CBZ
amine), the group can be removed using a technique known in the art
to afford the compound of formula I. More particularly, in
compounds of Formula I containing an amino group in the side chain
(e.g. R.sup.3 is aminoalkyl), the amino group is generally
protected to avoid undesired side reactions during the synthesis.
Protection can suitably be accomplished through the use of BOC,
CBZ, or a similar protective group.
[0242] Carboxylate compounds of the present invention in which a is
a single bond and X is (CH.sub.2).sub.1-3 can be prepared as
depicted in Scheme 2:
##STR00018##
In Scheme 2, ester G is obtained by reacting bicyclic urea
intermediate A with side chain alcohol F (wherein if necessary the
alcohol incorporates a protective group) in the presence of an
esterifying reagent (e.g., 1-2 equivalents of DCC or EDC) in the
presence of a catalyst (e.g., 0.05-0.25 equivalents of DMAP) in an
aprotic solvent (e.g., an ether such as diethyl ether or THF or a
haloalkane such as dichloromethane) at a temperature ranging from
about 0.degree. C. to 35.degree. C. until the reaction is complete
(e.g., about 1-24 hours) as monitored by TLC or HPLC. Intermediate
G can then be converted to a compound of the present invention by a
synthetic sequence (debenzylation, sulfation, and side chain
deprotection (if necessary)) analogous to the one outlined in
Scheme 1 for the synthesis of amide analogs.
[0243] Carboxamide compounds of the present invention in which a is
a single bond and X is CH.dbd.CH (see T below) and those in which a
is a double bond and X is CH.sub.2 (see W below) can be prepared as
depicted in Scheme 3:
##STR00019##
[0244] As shown in Scheme 3, protected amino acid J can be coupled
with amine B (1-2 molar equivalents) in solvent (e.g., a haloalkane
such as dry dichloromethane or chloroform) with stirring at room
temperature while sequentially adding triethylamine (1-2
equivalents), HOBT (1-2 equivalents), and EDC (1-2 equivalents)
under nitrogen. The resulting reaction mixture can then be stirred
at room temperature until the reaction is complete (e.g., in about
8 to 24 hours) and the intermediate amide recovered using known
techniques (e.g., concentrating the reaction mixture under vacuum
purifying the residue using column chromatography on silica gel or
HPLC). The BOC protective group can then be removed using methods
well known in the art to afford an amine which can be reductively
aminated with aldehyde L by reaction with a reducing agent such as
sodium cyanoborohydride (1-3 molar equivalents) or the like at a
temperature from 0.degree. C. to room temperature in an alcohol
solvent such as methanol, ethanol, or the like. The reaction
mixture can be stirred until the reaction is complete (e.g., in
about 1 to 24 hours), followed by recovering of amine K using known
techniques (e.g., concentrating the reaction mixture under vacuum
and purifying the residue via chromatography). Amine K can then be
acylated with M in the presence of a strong base such as DBU
(.about.1 equivalent) or the like in an aromatic hydrocarbon
solvent such as benzene, toluene, or the like to afford an
intermediate urea which is recovered using known techniques (e.g.,
washing the reaction mixture with aqueous acid, concentrating the
washed mixture under vacuum, and purifying the residue via
chromatography). The acetate protective group can then be removed
using well known techniques and the resulting primary hydroxy group
can be activated by reaction with .about.1 to 1.5 equivalents of
tosyl chloride, triflic anhydride, or the like in a
non-nucleophilic solvent (e.g., dichloromethane, ether, benzene,
etc.) at low temperature (e.g., from 0.degree. C. to 25.degree.
C.). The activated hydroxyl can then be treated with a
non-nucleophilic base such as DBU, potassium t-butoxide,
t-butyllithium, or the like at low temperature (e.g., from
0.degree. C. to 25.degree. C.) to afford cyclized intermediate Q,
which can be recovered and purified using standard work-up
procedures. Olefin metathesis techniques well known to those
skilled in the arts can be used to cyclize the di-olefin
intermediate Q. Thus, for example, Q can be treated with a
catalytic amount (0.05 to 0.25 equivalents) of a Grubbs olefin
methathesis catalyst in a suitable solvent (e.g., benzene, toluene,
tetrahydrofuran, or the like) at about 25.degree. C. to afford a
cyclohexene product, after which the protective group PG on the
hydroxylactam can be removed using well known techniques, and the
resulting hydroxylactam sulfated to afford the sulfate. For
example, a solution of hydroxylactam in an aprotic solvent (e.g., a
tertiary amide such as pyridine, DMF, or DMAC) can be treated with
a sulfur trioxide pyridine complex (2-10 equivalents) at about
25.degree. C. to afford the desired product which can be recovered
and purified using standard techniques to afford T. In cases where
there is no protective group in the side chain, the product of the
sulfation reaction is a compound of Formula I of the present
invention. When a protective group is incorporated in the amide
side chain (e.g., a benzyl amine or ether, BOC amine, or a CBZ
amine), the group can be removed using a technique known in the art
to afford the compound of Formula I. The beta-gamma olefin in
compound T can be isomerized into conjugation with the side chain
amide carbonyl by treating T with a non-nucleophilic base (e.g.,
potassium t-butoxide, sodium hydride, or the like) in a
non-nucleophilic solvent (e.g., t-butanol, tetrahydrofuran, ether,
or the like at 0.degree. C. to 25.degree. C. Alternatively, the
olefin can be isomerized into conjugation under acidic conditions
using an an acid such as trifluoromethanesulfonic acid or the like
or an acidic ion exchange resin in a non-nucleophilic solvent
(e.g., t-butanol, tetrahydrofuran, ether, or the like) at 0.degree.
C. to 25.degree. C. The resulting olefin isomer W can be recovered
and isolated using standard work-up techniques.
[0245] Generally speaking, when a chemical group in a compound is
referred to herein as "protected" or is said to incorporate a
"protective group", this means that the chemical group is employed
in a modified form to preclude undesired side reactions at the
protected site. Protective groups suitable for use in the
preparation of compounds of the present invention and techniques
for adding and removing such protective groups are well known in
the art and include those described in Protective Groups in Organic
Chemistry, ed. J. F. W. McOmie, Plenum Press, 1973 and in T. W.
Greene & P. G. M. Wuts, Protective Groups in Organic Synthesis,
John Wiley & Sons, 3.sup.rd edition, 1999, and 2.sup.nd
edition, 1991, the disclosures of which are herein incorporated by
reference in their entireties.
[0246] The present invention also includes a process (alternatively
referred to as Process P) for preparing a compound of Formula
P-II:
##STR00020##
which comprises:
[0247] (A) contacting a ketosulfoxonium glide of Formula P-I:
##STR00021##
with an iridum, rhodium, or ruthenium catalyst to obtain Compound
P-II; wherein: P.sup.G is an amine protective group selected from
the group consisting of carbamates and benzylamines; R.sup.U is
CH.sub.3 or phenyl; R.sup.V is CH.sub.3 or phenyl; R.sup.4 is H or
C.sub.1-4 alkyl; T' is H, Cl, Br, F, C.sub.1-3 alkyl, O--C.sub.1-3
alkyl, OH, NH.sub.2, N(H)--C.sub.1-3 alkyl, or N(--C.sub.1-3
alkyl).sub.2; p is zero, 1 or 2; q is zero, 1, or 2; and p+q=zero,
1, 2, or 3.
[0248] Compound P-II is an intermediate useful in the synthesis of
certain compounds of the present invention. The amine protective
group P.sup.G can be a carbamate (i.e., a protective group of
formula
##STR00022##
in which R is optionally substituted alkyl, allyl, optionally
substituted benzyl, or the like) or a benzylamine (i.e., a
protective group of formula
##STR00023##
in which Ar is optionally substituted phenyl). Suitable carbamate
and benzylamine protective groups and methods for their formation
and cleavage are described in Protective Groups in Organic
Chemistry, ed. J. F. W. McOmie, Plenum Press, 1973 and in T. W.
Greene & P.G.M. Wuts, Protective Groups in Organic Synthesis,
John Wiley & Sons, 3.sup.rd edition, 1999, and 2.sup.nd
edition, 1991. In one embodiment, P.sup.G is (1)
C(.dbd.O)--O--(CH.sub.2).sub.0-1--CH.dbd.CH.sub.2, (2)
C(.dbd.O)--O--CH.sub.2-phenyl in which the phenyl is optionally
substituted with from 1 to 3 substituents each of which is
independently halo, --NO.sub.2, --C.sub.1-4 alkyl, or
--O--C.sub.1-4 alkyl, (3) C(.dbd.O)--O--C.sub.1-4 alkyl, or (4)
CH.sub.2-phenyl in which the phenyl is optionally substituted with
from 1 to 3 substituents each of which is independently halo,
--NO.sub.2, --C.sub.1-4 alkyl, or --O--C.sub.1-4 alkyl. In another
embodiment, PG is t-butyloxycarbonyl (Boc), allyloxycarbonyl
(Alloc), benzyloxycarbonyl (Cbz), p-methoxybenzyloxycarbonyl,
p-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl,
p-chlorobenzyloxycarbonyl, 2,4-dichlorobenzyloxycarbonyl, or
benzyl. In still another embodiment, PG is Cbz.
[0249] Other embodiments of Compound P-II include the following:
(1) R.sup.U and R.sup.V are both CH.sub.3 or both phenyl; (2a) T'
is H or F; (2b) T' is H; (3a) R.sup.4 is H or CH.sub.3; (3b)
R.sup.4 is H. One or more of these embodiments (1) to (3) can be
combined with each other and/or with the embodiments described
above for P.sup.G, wherein each such combination is a separate
embodiment of Compound P-II.
[0250] Step A involves the intramolecular insertion of NH using a
ketosulfoxonium ylide to form a cyclic product. The ylide chemistry
employed in Step A provides a safety benefit with respect to
alternative methods that employ diazomethane (an explosion hazard)
to generate a diazoketone which can then be used in a cyclization.
Step A can also provide a high yield. For example, the yield of
Step A using a catalytic amount of [Ir(COD)Cl].sub.2 can be 85% or
higher.
[0251] Step A is conducted in an organic solvent. Suitable solvents
include toluene, dichloromethane, DCE, DMF, THF, chlorobenzene,
1,2-dichlorobenzene, cyclopentylmethyl ether, acetonitrile, IPAc,
nitromethane, trifluoromethylbenzene, methyl ethyl ketone, DME, and
2-MeTHF. A preferred solvent is toluene.
[0252] The cyclization in Step A is conducted in the presence of an
Ir, Rh, or Ru catalyst. Suitable catalysts include
[Ir(COD)Cl].sub.2, RuCl.sub.2(PPh.sub.3).sub.3,
Ru(DMSO).sub.4Cl.sub.2, [RuCl.sub.2(cymene)].sub.2,
[RuI.sub.2(cymene)].sub.2, (cyclopentadienyl)Ru(PPh.sub.3).sub.2,
(indene)RuCl(PPh.sub.3).sub.2, Rh.sub.2(OAc).sub.4,
Rh.sub.2(TFA).sub.4, (COD).sub.2IrBF.sub.4,
IrCl(CO)(PPh.sub.3).sub.2, IrCl(CO).sub.3, Ir(COD)(acac),
Ir(CO)(acac), (methylcyclopentadienyl)(COD)Ir, or
((cyclohexyl).sub.3P).sub.3(COD)Ir(pyridine). A class of suitable
catalysts consists of [Ir(COD)Cl].sub.2,
RuCl.sub.2(PPh.sub.3).sub.3, Ru(DMSO).sub.4Cl.sub.2,
[RuCl.sub.2(cymene)].sub.2, [RuI.sub.2(cymene)].sub.2,
(cyclopentadienyl)Ru(PPh.sub.3).sub.2,
(indene)RuCl(PPh.sub.3).sub.2, Rh.sub.2(OAc).sub.4,
Rh.sub.2(TFA).sub.4. A preferred catalyst is [Ir(COD)Cl].sub.2. The
catalyst is typically employed in an amount in a range of from
about 0.25 to 5 mole percent based on the amount of Compound P-I,
and is more typically employed in an amount in a range of from
about 0.5 to about 2 mole percent.
[0253] The reaction in Step A can suitably be conducted at a
temperature in a range of from about 50.degree. C. to about
130.degree. C. and is typically conducted at a temperature in a
range of from about 70.degree. C. to about 110.degree. C.
[0254] An embodiment of Process P comprises Step A as just
described above wherein PG is Cbz, to obtain Compound P-IIa
(=Compound P-II in which PG is replaced with Cbz), and further
comprises:
[0255] (B) treating Compound P-IIa with a reducing agent to obtain
a compound of Formula P-III:
##STR00024##
and
[0256] (C) contacting Compound P-III with a sulfonyl halide of
formula R --SO.sub.2W in the presence of a tertiary amine base to
obtain a compound of Formula P-IV:
##STR00025##
wherein W is halogen; and R is (1) phenyl optionally substituted
with from 1 to 3 substituents each of which is independently
C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, O--C.sub.1-4 alkyl,
O--C.sub.1-4 haloalkyl, Cl, Br, F, or NO.sub.2, (2) C.sub.1-4
alkyl; or (3) C.sub.1-4 haloalkyl.
[0257] Step B is conducted in an organic solvent. Suitable solvents
include toluene, dichloromethane, THF, isopropyl alcohol, and
acetonitrile. Preferred solvents are toluene and THF.
[0258] Suitable reducing agents in Step B include LiBH.sub.4,
NaBH.sub.4, KBH.sub.4, (Me.sub.4N)BH4, LiAlH(O-t-Bu).sub.3,
LiBH(OEt).sub.3, and Al(O-i-Pr).sub.3/IPA. A class of suitable
reducing agents consists of LiBH.sub.4, NaBH.sub.4, and KBH.sub.4.
Preferred reducing agents include LiBH.sub.4 and NaBH.sub.4. The
reducing agent is typically employed in an amount in a range of
from about 1 to about 2 equivalents per equivalent of Compound
P-IIa, and is more typically employed in an amount in a range of
from about 1 to about 1.3 equivalents.
[0259] The reaction in Step B can suitably be conducted at a
temperature in a range of from about -20.degree. C. to about
40.degree. C. and is typically conducted at a temperature in a
range of from about -15.degree. C. to about 0.degree. C.
[0260] Step C is conducted in an organic solvent. Suitable solvents
include dichloromethane, THF, ethyl acetate, and MTBE. A preferred
solvent is dichloromethane.
[0261] Exemplary sulfonyl halides suitable for use in Step C
include methanesulfonyl chloride, chloromethanesulfonyl chloride,
dichloromethanesulfonyl chloride, benzenesulfonyl chloride,
p-trifluoromethylbenzenesulfonyl chloride, p-toluenesulfonyl
chloride, p-bromobenzenesulfonyl chloride, p-fluorobenzenesulfonyl
chloride, and p-methoxybenzenesulfonyl chloride. A class of
suitable sulfonyl halides consists of chloromethanesulfonyl
chloride, p-trifluoromethylbenzenesulfonyl chloride and
p-bromobenzenesulfonyl chloride. A preferred sulfonyl halide is
p-trifluoromethylbenzenesulfonyl chloride. The sulfonyl halide is
typically employed in an amount in a range of from about 1 to about
2 equivalents per equivalent of Compound P-III, and is more
typically employed in an amount in a range of from about 1 to about
1.5 equivalents (e.g., about 1.3 equivalents).
[0262] The tertiary amine in Step C is suitably a tri-C.sub.1-4
alkylamine. A class of suitable amines consists of TEA, DIPEA, and
diethylisopropylamine. DIPEA is a preferred base. The base is
typically employed in an amount in a range of from about 1 to about
3 equivalents per equivalent of Compound P-III, and is more
typically employed in an amount in a range of from about 1.1 to
about 2 equivalents (e.g., about 1.8 equivalents).
[0263] The reaction in Step C can suitably be conducted at a
temperature in a range of from about 0.degree. C. to about
40.degree. C. and is typically conducted at a temperature in a
range of from about 10.degree. C. to about 25.degree. C.
[0264] Another embodiment of Process P comprises Steps A, B, and C
as just described above wherein PG is Cbz, to obtain Compound P-IV,
and further comprises:
[0265] (D) contacting Compound P-IV with
N-Boc-O-benzylhydroxylamine in the presence of a base to obtain a
compound of Formula P-V:
##STR00026##
and
[0266] (E) treating Compound P-V with an acid to obtain a compound
of Formula P-VI:
##STR00027##
[0267] Step D is conducted in an organic solvent. Suitable solvents
include DMAC, DMF, NMP, THF and DME. A preferred solvent is
NMP.
[0268] Suitable bases in Step D include Li t-butoxide, Na
t-butoxide, K t-butoxide, cesium carbonate, KHMDS, and NaHMDS. A
class of suitable bases consists of Li t-butoxide, Na t-butoxide
and K t-butoxide. A preferred base is K t-butoxide. The base is
typically employed in an amount in a range of from about 1 to about
2 equivalents per equivalent of Compound P-IV, and is more
typically employed in an amount in a range of from about 1 to about
1.5 equivalents (e.g., about 1.3 equivalents).
[0269] The N-Boc-O-benzylhydroxylamine is typically employed in
Step D in an amount in a range of from about 1 to about 2
equivalents per equivalent of Compound P-IV, and is more typically
employed in an amount in a range of from about 1 to about 1.5
equivalents (e.g., about 1.3 equivalents).
[0270] The reaction in Step D can suitably be conducted at a
temperature in a range of from about 30.degree. C. to about
60.degree. C. and is typically conducted at a temperature in a
range of from about 35.degree. C. to about 45.degree. C.
[0271] Step E is conducted in an organic solvent. Suitable solvents
include DCM and acetonitrile.
[0272] Suitable acids in Step E include sulfonic acids. Suitable
acids in Step E include methanesulfonic acid, trifluoromethane
sulfonic acid, chloromethanesulfonic acid, benzenesulfonic acid,
p-toluenesulfonic acid, p-bromobenzenesulfonic acid,
p-methoxybenzenesulfonic acid, and p-trifluoromethylbenzenesulfonic
acid. A class of suitable acids consists of p-toluenesulfonic acid
and methanesulfonic acid. A preferred acid is methanesulfonic acid.
The acid is typically employed in an amount in a range of from
about 1 to about 6 equivalents per equivalent of Compound P-V, and
is more typically employed in an amount in a range of from about 3
to about 5 equivalents.
[0273] The reaction in Step E can suitably be conducted at a
temperature in a range of from about 25.degree. C. to about
60.degree. C. and is typically conducted at a temperature in a
range of from about 30.degree. C. to about 40.degree. C.
[0274] Another embodiment of Process P comprises Steps A, B, C, D
and E as just described above wherein PG is Cbz, to obtain Compound
P-VI, and further comprises:
[0275] (F) contacting Compound P-VI with phosgene, diphosgene or
triphosgene in the presence of a tertiary amine base, and then
adding an aqueous solution of acid to obtain a compound of Formula
P-VII:
##STR00028##
and
[0276] (G) contacting Compound P-VII with a source of hydrogen in
the presence of a hydrogenolysis catalyst and in the presence of a
Boc-producing agent to obtain a compound of Formula P-VIII:
##STR00029##
[0277] Step F is conducted in an organic solvent. Suitable solvents
include DCM and acetonitrile. A preferred solvent is DCM.
[0278] Suitable acids include in Step F include hydrochloric acid,
sulfuric acid, trifluoroacetic acid, and phosphoric acid. A
preferred acid is phosphoric acid. The acid is typically employed
in an amount in a range of from about 1 to about 6 equivalents per
equivalent of Compound P-VI, and is more typically employed in an
amount in a range of from about 3 to about 5 equivalents (e.g.,
about 3.2 equivalents).
[0279] The tertiary amine in Step F is suitably a tri-C.sub.1-4
alkylamine. A class of suitable amines consists of TEA, DIPEA, and
diethylisopropylamine. DIPEA is a preferred base. The base is
typically employed in an amount in a range of from about 1 to about
6 equivalents per equivalent of Compound P-VI, and is more
typically employed in an amount in a range of from about 3 to about
5 equivalents (e.g., about 3.2 equivalents).
[0280] The triphosgene, diphosgene, or phosgene is typically
employed in Step F in an amount in a range of from about 0.5 to 1
equivalents per equivalent of Compound P-VI, and is more typically
employed in an amount in a range of from about 0.7 to about 1
equivalent (e.g., about 0.8 equivalent). Triphosgene is preferred
over diphosgene and phosgene.
[0281] The contacting of Compound P-VI with triphosgene,
diphosgene, or phosgene in Step F can suitably be conducted at a
temperature in a range of from about -15.degree. C. to about
0.degree. C. and is typically conducted at a temperature in a range
of from about 35.degree. C. to about 45.degree. C. The subsequent
addition and reaction with the acid can suitably be conducted at a
temperature in a range of from about 0.degree. C. to about
25.degree. C.
[0282] Step G is conducted in an organic solvent. Suitable solvents
include ethyl acetate, DMAC, t-butanol, and THF. A preferred
solvent is THF.
[0283] Suitable Boc-producing agents in Step G include di-t-butyl
carbonate, t-butylchloroformate, BOC-ON and BOC-OSN. A preferred
agent is d-t-butyl carbonate. The agent is typically employed in an
amount in a range of from about 0.9 to about 3 equivalents per
equivalent of Compound P-VII, and is more typically employed in an
amount in a range of from about 0.9 to 1.5 equivalents (e.g., from
about 0.95 to about 1.1 equivalents).
[0284] The source of hydrogen in Step G is typically hydrogen gas,
optionally in admixture with a carrier gas that is chemically inert
under the reaction conditions employed in Step G (e.g., nitrogen or
a noble gas such as helium or argon). The pressure is not a
critical aspect in Step G, although atmospheric and
superatmospheric pressures tend to be expedient. The pressure
typically is at least about 2 psig (about 115 kPa). The hydrogen
source can alternatively be a hydrogen-transfer molecule such as
ammonium formate, cyclohexene, or cyclohexadiene.
[0285] The uptake of hydrogen is not a critical process parameter,
although at least a stoichiometric amount of hydrogen gas or other
hydrogen source is typically employed.
[0286] The hydrogenolysis catalyst comprises a supported or
unsupported Group 8 metal or a supported or unsupported compound,
salt or complex of a Group 8 metal. The catalyst typically employed
in Step G is supported or unsupported Pd metal or a supported or
unsupported Pd compound, salt or complex. Suitable catalyst
supports include carbon, silica, alumina, silicon carbide, aluminum
fluoride, and calcium fluoride. A class of suitable catalysts
consists of Pd black (i.e., fine metallic palladium particles),
Pd(OH).sub.2, and Pd/C (i.e., palladium on a carbon support). Pd/C
is a preferred hydrogenolysis catalyst. The catalyst is typically
employed in an amount in a range of from about 5 to about 20 wt. %
relative to the amount of Compound VI, and is more typically
employed in an amount in a range of from about 5 to about 15 wt. %
(e.g., about 10 wt. %).
[0287] The reaction in Step G can suitably be conducted at a
temperature in a range of from about 10.degree. C. to about
50.degree. C. and is typically conducted at a temperature in a
range of from about 15.degree. C. to about 30.degree. C.
[0288] A sub-embodiment of Process P comprises Step A as just
described wherein the compound of Formula P-II is Compound p-2:
##STR00030##
wherein Step A comprises:
[0289] (A) contacting ketosulfoxonium ylide p-1:
##STR00031##
with a catalyst selected from the group consisting of iridium
cyclooctadiene chloride dimer, RuCl.sub.2(PPh.sub.3),
Ru(DMSO).sub.4Cl.sub.2, and Rh.sub.2(TFA).sub.4, to obtain Compound
p-2.
[0290] Another sub-embodiment of Process P comprises Step A as just
described in the preceding sub-embodiment to obtain Compound p-2,
and further comprises:
[0291] (B) treating Compound p-2 with a reducing agent selected
from the group consisting of Li borohydride, Na borohydride and K
borohydride, to obtain Compound p-3:
##STR00032##
and
[0292] (C) contacting Compound p-3 with a sulfonyl halide of
formula R --SO.sub.2W in the presence of a tri-C.sub.1-4 alkylamine
base to obtain a compound of Formula p-4:
##STR00033##
wherein W is chlorine; and R is methyl, chloromethyl, phenyl,
4-bromophenyl, 4-trifluoromethylphenyl, or 4-methylphenyl.
[0293] Another sub-embodiment of Process P comprises Steps A, B and
C as just described in the preceding sub-embodiment to obtain
Compound p-4, and further comprises:
[0294] (D) contacting Compound p-4 with N-Boc-O-benzylhydroxylamine
in the presence of a a base selected from the group consisting of
Li t-butoxide, Na t-butoxide, K t-butoxide and K amyloxide to
obtain Compound p-5:
##STR00034##
and
[0295] (E) treating Compound p-5 with an acid selected from the
group consisting of methanesulfonic acid, chloromethanesulfonic
acid, p-toluenesulfonic acid and benzenesulfonic acid to obtain a
compound of Formula p-6:
##STR00035##
[0296] Another sub-embodiment of Process P comprises Steps A, B, C,
D and E as just described in the preceding sub-embodiment to obtain
Compound p-6, and further comprises:
[0297] (F) contacting Compound p-6 with triphosgene in the presence
of a tri-C.sub.1-4 alkylamine base, and then adding an aqueous
solution of phosphoric acid to obtain Compound p-7:
##STR00036##
and
[0298] (G) contacting Compound p-7 with hydrogen in the presence of
a Pd catalyst and a Boc-producing agent selected from the group
consisting of di-t-butylcarbonate and BOC-ON to obtain Compound
p-8:
##STR00037##
[0299] The solvents, agents, catalysts, reaction amounts, reaction
temperatures, etc. described above for Steps A to F in Process P
leading to Compound P-VIII and its embodiments are applicable to
Steps A to F set forth in the preceding sub-embodiments leading to
Compound p-8, except where express limitations are placed upon one
or more of these variables in the sub-embodiments. For example, the
sub-embodiment of Process P describing the preparation of Compound
p-2 from Compound p-1 restricts the catalyst employed in Step A to
a specific group of 1r, Ru and Rh catalysts. Accordingly, the
broader disclosure of suitable catalysts provided for in Process P
as originally set forth above does not apply to this
sub-embodiment.
[0300] It is to be understood that the solvents, agents, catalysts,
reaction amounts, reaction temperatures, etc. described above with
respect to Process P and its embodiments and sub-embodiments are
intended only to illustrate, not limit, the scope of the process.
For example, the organic solvent employed in any of Steps A to G
can be any organic substance which under the reaction conditions
employed in the step of interest is in the liquid phase, is
chemically inert, and will dissolve, suspend, and/or disperse the
reactants and any reagents so as to bring the reactants and
reagents into contact and to permit the reaction to proceed.
Similar considerations apply to the choice of bases, catalysts, and
other reagents employed in the process steps. Furthermore, each of
the steps can be conducted at any temperature at which the reaction
forming the desired product can detectably proceed. The reactants,
catalysts and reagents in a given step can be employed in any
amounts which result in the formation of at least some of the
desired product. Of course, a high conversion (e.g., at least about
60% and preferably higher) of starting materials in combination
with a high yield (e.g., at least about 50% and preferably higher)
of desired products is typically the objective in each step, and
the choice of solvents, agents, catalysts, reaction amounts,
temperatures, etc. that can provide relatively good conversions and
yields of product are preferred, and the choices that can provide
optimal conversions and yields are more preferred. The particular
solvents, agents, catalysts, reaction amounts, reaction
temperatures, etc. described above with respect to Process P and
its embodiments and sub-embodiments can provide good to optimum
conversions and yields.
[0301] The present invention also includes a compound selected from
the group consisting of:
##STR00038##
wherein: P.sup.G is an amine protective group selected from the
group consisting of carbamates and benzylamines; R.sup.U is
CH.sub.3 or phenyl; R.sup.V is CH.sub.3 or phenyl; R.sup.4 is H or
C.sub.1-4 alkyl; T' is H, Cl, Br, F, C.sub.1-3 alkyl, O--C.sub.1-3
alkyl, OH, NH.sub.2, N(H)--C.sub.1-3 alkyl, or N(--C.sub.1-3
alkyl).sub.2; p is zero, 1 or 2; q is zero, 1, or 2; p+q=zero, 1,
2, or 3; and R is: [0302] (1) phenyl optionally substituted with
from 1 to 3 substituents each of which is independently C.sub.1-4
alkyl, C.sub.1-4 haloalkyl, O--C.sub.1-4 alkyl, O--C.sub.1-4
haloalkyl, Cl, Br, F, or NO.sub.2; [0303] (2) C.sub.1-4 alkyl; or
[0304] (3) C.sub.1-4 haloalkyl.
[0305] The present invention also includes a compound selected from
the group consisting of:
##STR00039##
wherein R is methyl, chloromethyl, phenyl, 4-bromophenyl,
4-trifluoromethylphenyl, or 4-methylphenyl.
[0306] The following examples serve only to illustrate the
invention and its practice. The examples are not to be construed as
limitations on the scope or spirit of the invention.
Preparative Example 1
(4R,6S)-3-(Benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carboxylic
acid
##STR00040## ##STR00041##
[0307] Step 1: 2-Allyl 1-tert-butyl
(2S,4S)-4-hydroxypyrrolidine-1,2-dicarboxylate
[0308] Di-tert-butyl dicarbonate (0.532 mL, 2.291 mmol) was added
to a solution of cis-4-hydroxy-L-proline (265 mg, 2.02 mmol) in DMF
(5 mL) and aqueous sodium hydroxide (2 mL, 2 mmol). The reaction
mixture was stirred at room temperature overnight. Allyl bromide
(0.18 mL, 2.08 mmol) was added and the resulting mixture was
stirred at room temperature overnight. The reaction mixture was
diluted with ethyl acetate and washed with dilute aqueous HCl,
water, saturated sodium bicarbonate and brine, dried over magnesium
sulfate, filtered and concentrated under vacuum to afford the title
compound as a clear oil.
Step 2: Allyl (4S)-4-hydroxy-L-prolinate-hydrochloride salt
[0309] Hydrochloric acid (4.2 M solution in dioxane, 5 mL, 21 mmol)
was added to a solution of 2-allyl 1-tert-butyl
(2S,4S)-4-hydroxypyrrolidine-1,2-dicarboxylate (1.05 g, 4.87 mmol)
in dichloromethane (20 mL). The resulting mixture was stirred at
room temperature for 4 hours then concentrated under vacuum to
afford the title compound.
Step 3: Allyl (4S)-4-hydroxy-1-(trifluoroacetyl)-L-prolinate
[0310] THF (18 mL) was added to allyl
(4S)-4-hydroxy-L-prolinate-hydrochloride salt (1.011 g, 4.87 mmol).
The resulting suspension was cooled to 0.degree. C. and
triethylamine (3.0 mL, 21.5 mmol) was added followed by
trifluoroacetic anhydride (2 mL, 14.2 mmol). The resulting mixture
was stirred at 0.degree. C. for 30 minutes then water was added.
The resulting solution was stirred at room temperature for 30
minutes then diluted with ethyl acetate and washed with 1N HCl
solution, water, dilute sodium bicarbonate solution, dried over
magnesium sulfate, filtered and concentrated under vacuum. In order
to hydrolyze any trifluoroacetate ester that may have formed as a
by-product, the residue was taken up in tetrahydrofuran (11.5 mL)
and water (11.5 mL). The resulting cloudy solution was stirred at
room temperature for 7 hours. The resulting clear solution was
diluted with ethyl acetate and washed with 5% sodium bicarbonate
solution and brine, then dried over magnesium sulfate, filtered
concentrated under vacuum to afford crude product. The crude
product was purified by silica gel chromatography to afford the
title compound as a pale tan oil.
Step 4: Allyl
(4R)-4-[(benzyloxy)amino]-1-(trifluoroacetyl)-L-prolinate
[0311] A solution of allyl
(4S)-4-hydroxy-1-(trifluoroacetyl)-L-prolinate (844 mg, 3.16 mmol)
in acetonitrile (16 mL) was cooled to -10.degree. C. and
2,6-lutidine (0.62 mL, 5.32 mmol) was added followed by
trifluoromethanesulfonic anhydride (0.85 mL, 5.15 mmol). After
addition, the temperature was allowed to warm to 0.degree. C. The
reaction mixture was stirred at 0.degree. C. for 1 hour then
O-benzylhydroxylamine (1 mL, 8.67 mmol) was added followed by
2,6-lutidine (0.62 mL, 5.32 mmol). The reaction mixture was allowed
to warm to room temperature overnight. The reaction mixture was
then diluted with ethyl acetate and washed with 5% sodium
bicarbonate solution and brine, dried over magnesium sulfate,
filtered and concentrated under vacuum. The tan oil residue (2.57
g) was chromatographed on silica gel eluted initially with 95:5
dichloromethane:ethyl acetate and finally with 80:20
dichloromethane:ethyl acetate to afford the title compound as a
pale yellow solid.
Step 5: Allyl (4R)-4-[(benzyloxy)amino]-L-prolinate
[0312] A solution of allyl
(4R)-4-[(benzyloxy)amino]-1-(trifluoroacetyl)-L-prolinate (1.19 g,
3.20 mmol) in methanol (9.5 mL) was added slowly to a solution of
sodium borohydride (312 mg, 8.25 mmol) in methanol (9.5 mL) at
-10.degree. C. The reaction mixture was allowed to warm to
0.degree. C. slowly then stirred at 0.degree. C. for 3 hours.
Additional sodium borohydride (0.29 g, 7.67 mmol) was added at
0.degree. C. and the reaction mixture was stirred at 0.degree. C.
for three hours then silica gel (to pre-absorb the crude product
for chromatography) was added and the solvent removed under vacuum.
The residue was chromatographed on silica gel eluted with 15:9:1
dichloromethane:ethyl acetate:methanol to afford the title compound
as a colorless oil.
[0313] Step 6 2-Allyl 1-tert-butyl
(2S,4R)-4-[(benzyloxy)amino]pyrrolidine-1,2-dicarboxylate
[0314] A solution of allyl (4R)-4-[(benzyloxy)amino]-L-prolinate
(1.2 g, 4.34 mmol) in dichloromethane (29 mL) was added to
di-tert-butyl dicarbonate (1.1 mL, 4.34 mmol) and the resulting
mixture was stirred at room temperature overnight. The reaction
mixture was concentrated under vacuum and the residue was
chromatographed on silica gel eluted with hexane then with 4:1
hexane:ethyl acetate to afford the title compound as a clear
gum.
Step 7: 2-Allyl 1-tert-butyl
(2S,4R)-4-{(benzyloxy)[(trichloromethoxy)carbonyl]amino}-pyrrolidine-1,2--
dicarboxylate
[0315] Diphosgene (0.1 mL, 0.804 mmol) was added slowly to a
solution of 2-allyl 1-tert-butyl
(2S,4R)-4-[(benzyloxy)amino]pyrrolidine-1,2-dicarboxylate (261 mg,
0.745 mmol) and triethylamine (0.13 mL, 0.933 mmol) in
dichloromethane (4 mL) at 0.degree. C. The reaction mixture was
stirred at 0.degree. C. for 4 hours then allowed to stand at room
temperature overnight. The reaction mixture was chromatographed on
silica gel eluted first with hexane then with 4:1 hexane:ethyl
acetate to afford the title compound as a clear gum.
Step 8: Allyl
(4R,6S)-3-(benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carboxylate
[0316] Hydrochloric acid (4.2 M solution in dioxane, 16 mL, 70.4
mmol) was added to 2-allyl 1-tert-butyl
(2S,4R)-4-{(benzyloxy)[(trichloromethoxy)carbonyl]amino}-pyrrolidine-1,2--
dicarboxylate (80 mg, 1.49 mmol). The resulting mixture was stirred
at room temperature overnight then the solvent was removed under
vacuum. Dichloromethane (82 mL) was added to the residue followed
by triethylamine (0.62 mL, 4.45 mmol). The resulting mixture was
stirred at room temperature overnight then the solvent was removed
under vacuum. The residue was chromatographed on silica gel (ISCO
chromatography system) using a gradient from hexane for 2 minutes
to 7:3 hexane:ethyl acetate over 6 minutes, hold for 3 minutes and
go to 100% EtOAc over 8 minutes to afford the title compound as a
clear oil.
Step 9:
(4R,6S)-3-(Benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carbo-
xylic acid
[0317] Sodium 2-ethylhexanoate (0.5 M in ethyl acetate, 2.5 mL,
1.25 mmol) was added to a solution of allyl
(4R,65)-3-(benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carboxylate
(459 mg, 1.52 mmol),
1,1'-bis(diphenyl-phosphino)ferrocene-palladium(II)dichloride
dichloromethane complex (116 mg, 0.14 mmol) in tetrahydrofuran (7.6
mL). The reaction mixture was stirred at room temperature for 2
hours (precipitate formed). Acetone (37 mL) was added. The
resulting mixture was stirred at room temperature for 2 hours and
the mixture was centrifuged. The solid was collected and washed
with acetone and ether and dried under vacuum to afford the title
compound as a tan solid. LC-MS (positive ionization) m/e 363
(M+H)
Example 1
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane--
2-carboxamide
##STR00042##
[0318] Step 1: tert-butyl
4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl-
}amino)piperidine-1-carboxylate
[0319] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (0.141 g, 0.509 mmol) (note: this intermediate is disclosed
in U.S. Pat. No. 7,112,592 Example 32b) in dry dichloromethane (3
mL) was added 4-amino-1-BOC-piperidine (0.1532 g, 0.765 mmol),
triethylamine (0.16 mL, 1.148 mmol), HOBT (0.1145 g, 0.748 mmol),
and EDC (0.1455 g, 0.759 mmol) sequentially at room temperature
under nitrogen. The reaction was stirred at room temperature
overnight. The reaction mixture was concentrated under vacuum and
the residue was purified by HPLC (30.times.100 mm Waters Sunfire
column; 5 micron; 35 mL/minute; 210 nM; 15% to 100%
CH.sub.3CN+0.05% TFA/water+0.05% TFA over 15 minutes; desired
product elutes at 50% CH.sub.3CN+0.05% TFA/water+0.05% TFA) to
afford the title compound.
Step 2: tert-Butyl
4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}ami-
no)piperidine-1-carboxylate
[0320] Palladium on carbon (30.5 mg; 10% Pd/C) was added to a
solution of tert-butyl
4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl-
}amino)piperidine-1-carboxylate (151 mg, 0.33 mmol) in methanol (3
mL), and the resulting mixture was stirred under hydrogen (balloon)
for 3 hours. TLC analysis showed the reaction was complete. The
reaction mixture was filtered through a microfilter and the
filtrate was concentrated under vacuum to afford the title compound
as a yellow oil.
Step 3:
N,N,N-Tributylbutan-1-aminium[({(2S,5R)-7-oxo-2-[(piperidin-4-ylam-
ino)carbonyl]-1,6-diazabicyclo[3.2.1]oct-6-yl}oxy)sulfonyl]oxidanide
[0321] To a solution of tert-butyl
4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}ami-
no)piperidine-1-carboxylate (36 mg, 0.098 mmol) in pyridine (0.5
mL) was added sulfur trioxide pyridine complex (70 mg, 0.440 mmol).
The mixture was stirred at room temperature under nitrogen
overnight. LC/MS analysis showed incomplete reaction. The reaction
was filtered and the solids were washed with dry pyridine and
dichloromethane. The filtrate was collected and concentrated under
vacuum. The residue was redissolved in dry pyridine (0.75 mL) and
sulfur trioxide pyridine complex (31 mg) was added followed by
activated 4 A molecular sieves. The reaction was stirred for 4
hours but there was little change by LC/MS. The reaction was
filtered and the sieves were washed with dichloromethane. The
filtrate was concentrated in vacuo and suspended in saturated
aqueous potassium dihydrogen phosphate solution. The resulting
mixture was washed with ethyl acetate. The aqueous layer was
collected and tetrabutylammonium hydrogen sulfate (0.034 mg, 0.098
mmol) was added. The mixture was stirred for 10 minutes then
extracted with EtOAc (4.times.). The organic layers were combined,
dried over sodium sulfate, and concentrated in vacuo to afford the
title compound as a yellow oil.
Step 4:
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1-
]octane-2-carboxamide
[0322] To a solution of
N,N,N-tributylbutan-1-aminium[({(2S,5R)-7-oxo-2-[(piperidin-4-ylamino)car-
bonyl]-1,6-diazabicyclo[3.2.1]oct-6-yl}oxy)sulfonyl]oxidanide (22.4
mg, 0.050 mmol) in anhydrous dichloromethane (2 mL) at 0.degree. C.
under nitrogen was added trifluoroacetic acid (0.1 mL, 1.298 mmol)
dropwise. The reaction mixture was stirred for 1 hour then
concentrated under vacuum. Ether was added to the residue and the
resulting white precipitate was collected by centrifugation. The
solid was washed with ether (2.times. to afford the title compound
contaminated with tetrabutylammonium hydrogen sulfate and pyridine.
The solid was triturated with acetonitrile (2.times.) and the white
solid was collected by centrifugation to afford the title compound
as a white solid. LC-MS (negative ionization) m/e 347 (M-H); LC-MS
(positive ionization) m/e 349 (M+H), 381 (M+Na); .sup.1H NMR (600
MHz, D.sub.2O; unreferenced) (.delta., ppm) 4.19 (1H, br d, J=2.5
Hz), 3.98-4.06 (2H, m), 3.47 (2H, br d, J=13 Hz), 3.31 (1H, br d,
J=12 Hz), 3.12 (2H, br dd, J=13, 3 Hz), 3.06 (1H, d, J=12 Hz),
2.04-2.21 (m, 4H), 1.87-1.95 (1H, m), 1.72-1.83 (m, 3H).
Example 1A
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane--
2-carboxamide
##STR00043##
[0323] Step 1: tert-butyl
4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl-
}amino)piperidine-1-carboxylate
[0324] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (1.484 g, 5.37 mmol) in dry dichloromethane (60 ml) was
added triethylamine (1.88 ml, 13.49 mmol),
2-chloro-1-methylpyridinium iodide (1.60 g, 6.26 mmol), and
4-amino-1-BOC-piperidine (1.30 g, 6.49 mmol) sequentially at room
temperature under nitrogen. The reaction was then heated to
50.degree. C. for 1 hour. The reaction mixture was concentrated
under vacuum and purified by silica gel chromatography on an Isco
Combiflash (40 g silica gel, 40 mL/min, 254 nM, 15% to 100%
EtOAc/hexane over 14 column volumes then 100% EtOAc for 4 column
volumes; title compound eluted at 65% ethyl acetate/hexane) to
afford the title compound as a pale orange solid.
Step 2: tert-butyl
4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}ami-
no)piperidine-1-carboxylate
[0325] Palladium on carbon (394 mg; 10% Pd/C) was added to a
solution of the product of step 1 (1.81 g, 3.95 mmol) in methanol
(50.6 mL) and the resulting mixture was stirred under hydrogen
(balloon) overnight. LC/MS analysis indicated the reaction was not
complete. Acetic acid (6 drops) and additional catalyst (159 mg of
10% Pd/C) were added to the reaction and the resulting mixture was
stirred under hydrogen (balloon) for an additional 90 minutes.
Additional catalyst (0.2085 g of 10% Pd/C) was added to the
reaction and stirring under hydrogen was continued for an
additional 2.5 hours at which time the reaction was judged complete
by LC-MS analysis. The reaction was filtered through a celite pad
and the collected solid was washed well with MeOH. The filtrate was
concentrated under vacuum to afford the title compound as a
colorless oil which was used without purification in the next
step.
Step 3:
tert-butyl-4-({[(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]-
oct-2-yl]carbonyl}amino)piperidine-1-carboxylate
[0326] To a solution of the product of step 2 (1.455 g, 3.95 mmol;
theoretical yield of step 2) in dry pyridine (30 mL) was added
sulfur trioxide pyridine complex (3.2 g, 20.11 mmol) at room
temperature under nitrogen. The resulting thick mixture was stirred
over the weekend. The reaction was filtered and the white insoluble
solids were washed well with dichloromethane. The filtrate was
concentrated in vacuo. The residue was further azeotroped with
toluene to remove excess pyridine to afford the title compound
which was used without purification in the next step.
Step 4:
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1-
]octane-2-carboxamide
[0327] To a mixture of the product of step 3 (1.772 g, 3.95 mmol;
theoretical yield of step 3) in dry dichloromethane (30 ml) at
0.degree. C. under nitrogen was slowly added trifluoroacetic acid
(6.1 ml, 79 mmol). Immediately the reaction became a solution.
After 1 hour, additional trifluoroacetic acid (8 ml) was added to
the reaction. The reaction was stirred at 0.degree. C. until judged
complete by LC-MS analysis then concentrated in vacuo. The residue
was triturated with ether (3.times.) to remove excess TFA and
organic impurities. The resulting white insoluble solid was
collected via centrifugation, dried in vacuo, then purified by
preparative HPLC (250.times.21.2 mm Phenomenex Synergi Polar-RP 80A
column; 10 micron; 35 mL/min.; 210 nM; 0% to 30% methanol/water
over 15 minutes; title compound eluted at 10% methanol/water).
Fractions containing the title compound were combined and
lyophilized overnight to afford the title compound as a white
solid. LC-MS (negative ionization mode) m/e 347 (M-H).
Example 1C
(2S,5R)-7-Oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane--
2-carboxamide
Step 1: Benzyl
4-[(tert-butoxycarbonyl)amino]piperidine-1-carboxylate
##STR00044##
[0329] 4-(N-BOC amino) piperidine (17 kg, 84.88 mol) was dissolved
in DCM (90 kg), triethylamine (10.14 kg, 100.16 mol) was added, and
the resulting solution was cooled to 0-5.degree. C. Benzyl
chloroformate (16.51 kg, 96.76 mol) was added over 45 minutes while
keeping the temperature at <25.degree. C., after which the
solution was aged for 30 minutes at 20.degree. C. 2 M HCl (61 kg,
118.13 mol) was then added over 10 minutes while keeping the
temperature at <25.degree. C. The mixture was agitated for 10
minutes and then the agitation was stopped and the phases were
allowed to separate. The phases were then separated from each
other, and the organic phase was distilled in vacuo to a volume of
35 L. Isopropyl acetate (89 kg) was then added, and the batch was
concentrated by vacuum distillation at less than 35.degree. C. to a
volume of .about.50 L to crystallize the title product. Heptane (47
kg) was then added over 10 minutes, and the resulting slurry cooled
to and aged at 20.degree. C. for 20 minutes, after which the aged
slurry was filtered, washed with heptane (17 kg), and dried by
N.sub.2 sweep on the filter to give the title product as a white
solid (24.7 kg, 87%). .sup.1H NMR (CDCl.sub.3) 7.33 (5H, m), 5.13
(2H, s), 4.47 (1H, m), 4.11 (2H, m), 3.61 (1H, m), 2.93 (2H, m),
1.94 (2H, m), 1.45 (9H, s) and 1.30 (2H, m).
Step 2: Benzyl 4-aminopiperidine-1-carboxylate
##STR00045##
[0331] 4-(N-BOC amino)-CBz piperidine (24.4 kg 73.42 mol), THF (65
kg) and 5 M HCl (23.0 kg, 110.13 mol) were combined and heated to
30-35.degree. C. for .about.2 hours, and then at 55.degree. C.
overnight. After cooling the reaction mixture to 10.degree. C.,
dichloromethane (97 kg) and 10M NaOH (7.97 kg, 145.12 mol) were
added, while keeping the temperature at <25.degree. C. The
phases were separated and the organic phase was washed with 25 wt %
NaCl solution (27.5 kg). The washed organic phase was distilled at
atmospheric pressure to a volume of 70 L. Dichloromethane (162 kg)
was then added, and the mixture was concentrated by distillation to
a volume of 120 L to give the title product as a solution in DCM
(17.2 kg. 100%.). .sup.1H NMR (CDCl.sub.3) 7.33 (5H, m), 5.14 (2H,
s), 4.14 (2H, br s), 2.87 (3H, m), 1.83 (2H, m), 1.66 (3H, m) and
1.28 (2H, m).
Step 3: Benzyl
4-{[1-(tert-butoxycarbonyl)-5-oxo-L-prolyl]amino}piperidine-1-carboxylate
##STR00046##
[0333] 2-Hydroxypyridine-N-oxide (811 g, 7.3 mol), L-pyroglutamic
acid (9.43 kg, 73 mol), benzyl 4-aminopiperidine-1-carboxylate
(17.1 kg in dichloromethane, volume 120 L, 73 mol) and
dichloromethane (80 kg) were mixed together and aged at 20.degree.
C. for 10 minutes to form a thick slurry.
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (16.8
kg, 87.6 mol) was added in portions to the slurry, while keeping
temperature at <30.degree. C. The slurry was then aged at
25.degree. C. for 30 minutes, after which 1M hydrochloric acid (94
kg, 85.5 mol) was added. The phases were left to settle overnight
and then separated, and the organic phase was then washed with 2M
sodium carbonate (109 kg) and then solvent switched to
acetonitrile, final volume 50 L. Toluene (88.2 kg) was added and
the batch cooled to 0.degree. C. Di-tert-butyl dicarbonate (18.32
kg, 83.95 mol) and 4-dimethylaminopyridine (223 g, 1.83 mol) were
added to the batch and the solution was warmed to 25.degree. C. and
aged overnight. The batch was then concentrated by distillation to
a volume of 80 L. Additional toluene (88.2 kg) was added and the
batch further concentrated to 50 L. Isopropyl acetate (30 kg) was
added and the resulting slurry was aged for 10 minutes. Heptane (70
kg) was then added dropwise to the slurry over 30 minutes, and the
slurry was aged for 30 minutes then filtered, washed with isopropyl
acetate/heptane (22.5 kg/17.4 kg), and then dried in vacuo at
55.degree. C. to afford the title product as a white solid (27.5
kg, 95.5 wt %, 82%). .sup.1H NMR (CDCl.sub.3) 7.33 (5H, m), 6.19
(1H, m), 5.13 (2H, s), 4.48 (1H, dd), 4.15 (2H, m), 3.97 (1H, m),
2.95 (2H, m), 2.73 (1H, d tr), 4.65 (1H, m), 2.61 (1H, m), 2.18
(2H, m), 1.45 (9H, s) and 1.30 (2H, m).
Step 4: Benzyl
4-({N-(tert-butoxycarbonyl)-6-[dimethyl(oxido)-.quadrature.
.lamda..sup.4-sulfanylidene]-5-oxo-L-norleucyl}amino)piperidine-1-carboxy-
late
##STR00047##
[0335] Potassium tert-butoxide (9.58 kg, 85.38 mol) was added in
three portions to a solution of trimethyl sulfoxonium iodide (18.79
kg, 85.38 mol) in DMF (115 kg) at 15-25.degree. C. The suspension
was aged at 20-25.degree. C. for one hour, followed by addition of
the starting material benzyl
4-{[1-(tert-butoxycarbonyl)-5-oxo-L-prolyl]amino}piperidine-1-carboxylate
(27.17 kg, 60.99 mol) in four portions over 30 minutes, then aged
for 30 minutes at 20.degree. C. Water (54 kg) and seed material (10
g) were added (note: crystallization will occur without the use of
seed, but the use of seed is preferred as it typically provides a
more consistent product and a better yield), and the suspension was
aged at 20.degree. C. for 30 minutes. 10% NaCl solution (543 kg)
was added over 1 hour while keeping the temperature at below
25.degree. C. The slurry was then cooled to 3.degree. C. over 1
hour and aged overnight at 3.degree. C., after which the slurry was
filtered, washed three times with water (136 L, 82 L, 82 L), and
dried under vacuum at 55.degree. C., to afford the title product as
a yellow solid (32.8 kg, 83%). .sup.1H NMR (CDCl.sub.3) 7.49 (1H,
br s), 7.33 (5H, m), 5.83 (1H, br s), 5.13 (2H, s), 4.48 (1H, s),
4.08 (3H, m), 3.96 (1H, m), 3.45 (3H, s), 3.41 (3H, s), 3.03 (2H,
m), 2.41 (1H, m), 2.24 (1H, m), 1.94 (4H, m), 1.68 (5H, s) and 1.44
(12H, s).
Step 5: Tert-butyl
(2S)-2-[({1-[(benzyloxy)carbonyl]piperidin-4-yl}amino)carbonyl]-5-oxopipe-
ridine-1-carboxylate
##STR00048##
[0337] Iridium cyclooctadiene chloride dimer (336.3 g, 0.502 mol)
in toluene (318 kg) was deoxygenated by degassing using 3 vacuum
degas cycles followed by subsurface sparging with nitrogen for 30
minutes, after which the solution was warmed to 105.degree. C. A
solution of the ylide starting material (27.0 kg, 50.22 mol) in DMF
(128 kg) at 25.degree. C. was deoxygenated by degassing using 3
vacuum degas cycles followed by subsurface sparging with nitrogen
for 30 minutes. The degassed solution was then added to the hot
catalyst solution over 30 minutes, while keeping temperature of the
reaction mixture above 102.degree. C. The reaction mixture was aged
at 105.degree. C. for 40 minutes, and then cooled to 20.degree. C.
The organic reaction mixture was washed twice with 5 wt % lithium
chloride solution (81 L.times.2) then with water (81 L). The
organic and aqueous phases were separated, and then toluene was
removed from the organic phase by distillation in vacuo to a volume
of 130 L and the distilled phase stored refrigerated then used
directly in the next step.
Step 6: tert-butyl
(2S,5S)-2-[({1-[(benzyloxy)carbonyl]piperidin-4-yl}amino)carbonyl]-5-hydr-
oxypiperidine-1-carboxylate
##STR00049##
[0339] Lithium borohydride solution (22.2 kg of 4.1 M solution in
THF, 101.9 mol) was diluted with THF (290 kg), after which methanol
(3.26 kg) was added at 20.degree. C. and the solution aged for 30
minutes before cooling to -4.degree. C. A solution of the ketone in
toluene (46.8 kg in .about.4 mL/g solution in toluene, 101.9 mol)
was added to the aged borohydride solution while maintaining the
reaction temperature at <0.degree. C. The reaction was quenched
with a solution of acetic acid (30.6 kg, 509.5 mol, dissolved in
183 kg methanol), while keeping the temperature at <20.degree.
C. The quenched reaction mixture was then aged at 20.degree. C. for
1 hour, after which it was concentrated in vacuo to a volume of 184
L. Methanol (203 kg) was added, and the batch distilled in vacuo to
a volume of 184 L. Isopropanol (294 kg) was added and the batch was
distilled in vacuo to a volume of 184 L, keeping the internal
temperature at .about.30.degree. C. Seed (5 g) was added (note:
crystallization will occur without the use of seed, but the use of
seed is preferred as it typically provides a more consistent
product and a better yield), and the batch was aged for 1 hour to
form a seedbed. Water (560 kg) was then added over .about.60
minutes, followed by the addition of isopropanol (111 kg). The
slurry was filtered, washed three times with MTBE (30 kg, 35 kg, 5
kg), and then dried in vacuo at 55.degree. C. to provide the title
product (26.74 kg, 57% yield from ylide). .sup.1H NMR (CDCl.sub.3)
7.33 (5H, m), 6.17 (1H, br s), 5.13 (2H, s), 4.61 (1H, m), 4.11
(3H, m), 3.94 (1H, m), 3.64 (1H, m), 2.98 (2H, m), 2.59 (1H, dd),
2.33 (1H, m), 1.94 (4H, m), 1.71 (1H, m), 1.63 (2H, m), 1.48 (9H,
s) and 1.35 (2H, m).
Step 7: Tert-butyl
(2S,5S)-2-[({1-[(benzyloxy)carbonyl]piperidin-4-yl}amino)carbonyl]-5-({[4-
-(trifluoromethyl)phenyl]sulfonyl}oxy)piperidine-1-carboxylate
##STR00050##
[0341] The alcohol starting material (26.6 kg, 57.7 mol) was
dissolved in dichloromethane (120 kg) and passed through a
cartridge of activated carbon. N,N-dimethylaminopyridine (1.06 kg,
8.66 mol) and TEA (11.1 kg, 109.63 mol) were added to the alcohol
solution, followed by the addition of
4-trifluoromethylbenzenesulfonyl chloride (18.0 kg, 73.6 mol) as a
solution in dichloromethane (30 kg) over 20 minutes at a
temperature of <25.degree. C. The batch was then aged for 3
hours, after which water (110 kg) was added, while keeping
temperature <25.degree. C. The phases were separated and the
organic phase was washed twice with water (80 kg.times.2), then
with aqueous HCl (15 L of 37 wt % concentrated. HCl in water (80
kg)). The organic layer was diluted with dichloromethane (75 kg)
and distilled in vacuo to 72 L. MTBE (157 kg) was then added and
the batch was distilled in vacuo to 170 L to crystallize the
product. The slurry was aged for 1 hour and then heptane (58 kg)
was added over a period of .about.20 minutes and the slurry was
aged at 20.degree. C. for 18 hours. The aged slurry was then
filtered, washed with heptane (20 kg) and MTBE (40 kg) and dried on
the filter using a stream of nitrogen for 24 hours to give the
title product (38.3 kg, 98%). .sup.1H NMR (CDCl.sub.3) 8.20 (2H,
d), 7.85 (2H, d), 7.33 (5H, m), 6.09 (1H, br s), 5.13 (2H, s), 4.59
(1H, m), 4.46 (1H, m), 4.10 (3H, m), 3.91 (1H, m), 2.96 (2H, m),
2.75 (1H, m), 2.33 (1H, m), 1.58 (1H, m), 1.48 (9H, s) and 1.35
(2H, m).
Step 8: Benzyl
4-[({(2S,5R)-5-[(benzyloxy)amino]piperidin-2-yl}carbonyl)amino]piperidine-
-1-carboxylate
##STR00051##
[0343] N-Boc-O-benzylhydroxylamine (8.65 g, 38.7 mmol, as a
solution in DMAC volume 38 mL) was added to a solution of potassium
tert-butoxide (4.35 g, 38.7 mmol) in DMAC (80 mL), while
maintaining the temperature between 18.degree. C. and 25.degree. C.
The solution was aged for 30 minutes after which time it became a
slurry. The sulfonate starting material (20 g, 29.9 mmol) dissolved
in DMAC (40 mL) was added to the slurry over 15 minutes at
20.degree. C., and the resulting mixture was heated to 40.degree.
C. for 3.5 hours and then left at 20.degree. C. overnight. Water
(350 mL) was added to the mixture while maintaining the temperature
at <30.degree. C. DCM (350 mL) was then added, and the phases
separated. The organic phase was washed three times with water (350
mL.times.3). The washed organic phase was then distilled under
atmospheric pressure to a volume of 90 mL, after which
methanesulfonic acid (10 mL) was added and the solution heated to
35-40.degree. C. for 8 hours. The solution was then cooled to
20.degree. C. and 2N NaOH (200 mL) added, followed by addition of
DCM (90 mL). The phases were separated, and the organic phase was
washed with water (90 mL), and then solvent switched at atmospheric
pressure to acetonitrile, volume 50 mL. p-Toluenesulfonic acid (4
g, 1 equiv. based on product assay) was added as a solution in
acetonitrile (40 mL) at 40.degree. C. to crystallize the product.
MTBE (45 mL) was then added and the slurry was cooled 20.degree.
C., aged at 20.degree. C. for 1 hour, and then filtered to give the
title product as a mono-tosylate crystalline salt (9.8 g, 53%).
.sup.1H NMR (CDCl.sub.3) 7.75 (1H, br s), 7.59 (2H, d), 7.36 (5H,
m), 7.20 (3H, m), 7.14 (2H, d), 6.98 (2H, d), 5.30 (1H, m), 5.10
(2H, m), 4.37 (2H, s), 3.88 (3H, m), 3.61 (2H, m), 3.23 (2H, m),
2.22 (3H, s), 1.65 (1H, m), 1.26 (5H, m) and 1.21 (3H, m).
Step 9: Benzyl
4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl-
}amino)piperidine-1-carboxylate
##STR00052##
[0345] Benzyl
4-[({(2S,5R)-5-[(benzyloxy)amino]piperidin-2-yl}carbonyl)amino]piperidine-
-1-carboxylate in the form of a tosylate salt (8.1 kg, 12.68 mol)
was slurried in dichloromethane (108 kg), after which 5 wt %
NaHCO.sub.3 (42 kg, 25.36 mol) was added and the resulting biphasic
mixture was stirred vigorously for 30 minutes. The phases were
separated, and the organic phase was washed with water (40.5 kg).
The organic phase was then distilled at atmospheric pressure to
.about.20 L, followed by the addition of DCM (108 kg). DIPEA (5.25
kg, 40.58 mol) was then added and the batch cooled to 0-5.degree.
C. Triphosgene (3.01 kg, 10.14 mol) was added in four portions,
while keeping the temperature at <10.degree. C. After 30
minutes, a dilute phosphoric acid solution (4.97 kg 85 wt %
phosphoric acid in 32 kg water) was added, and the batch aged at
20.degree. C. overnight. The phases were separated and the organic
phase was washed with 5 wt % NaHCO.sub.3 (26 kg) and water (25 kg).
The organic phase was then distilled at atmospheric pressure to 30
L. Ethanol (77 kg) was then added, followed by the addition of seed
(10 g). (Note: Crystallization will occur without the use of seed,
but the use of seed is preferred as it typically provides a more
consistent product and a better yield.) The slurry was distilled in
vacuo to a volume of 33 L, then heptane (55 kg) was added dropwise.
The slurry was then cooled to 0.degree. C., filtered, washed with
3:1 heptane:ethanol (30 L), and dried on the filter under nitrogen
stream to give the title product (5.90 kg, 94%). .sup.1H NMR
(CDCl.sub.3) 7.35 (10H, m), 6.57 (1H, d), 5.14 (2H, s), 5.07 (1H,
d), 4.92 (1H, d), 4.13 (2H, m), 3.95 (1H, m), 3.89 (1H, d), 3.31
(1H, s), 2.99 (3H, m), 2.65 (1H, d), 2.38 (1H, m), 1.94 (4H, m),
1.62 (2H, m) and 1.34 (2H, m).
Step 10: tert-Butyl
4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}ami-
no)piperidine-1-carboxylate
##STR00053##
[0347] Benzyl
4-({[(2R,5S)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl-
}amino)piperidine-1-carboxylate starting material (1.9 kg @ 97 wt
%) and Boc.sub.2O (0.776 kg) were charged to a glass bottle, and
the solids were dissolved in THF (15 L). The solution was then
charged to a hydrogenation reactor along with Pd(OH).sub.2 (184.3
g) and another portion of THF (10.8 L). The reaction was carried
out at 45 psig H.sub.2, 23.degree. C. for 5 hours. After the
reaction was complete as determined by HPLC analysis, the solution
was filtered through solka flok to remove the catalyst and the
filter cake was washed with THF. The filtrate and washes were then
solvent switched by vacuum distillation to EtOAc to a volume of 10
L. Approximately 30 L EtOAc was used during the solvent switch and
the THF level after a constant volume distillation (10 L at a
maximum temperature of 20.degree. C.) was determined by proton NMR
to be .about.4 mol % THF:EtOAc. The resulting EtOAc slurry was aged
at room temperature for 1 hour, after which hexanes (4 L) was added
over 1 hour at room temperature. The slurry was aged for an
additional 1 hour after which the supernatant concentration was
measured (target: .about.6 mg/g). The solids were then filtered and
washed with 60% EtOAc/hexanes solution (3.times.3 L) and dried
under vacuum and N.sub.2 at room temperature to afford the title
product (80% isolated yield). .sup.1H NMR (400 MHz, CDCl.sub.3):
8.60 (br s, 1H), 6.67 (d, J=8.2 Hz, 1H), 4.12-4.00 (m, 2H),
4.00-3.91 (m, 1H), 3.89 (d, J=7.8 Hz, 1H), 3.81-3.76 (m, 1H), 3.19
(dt, J=11.2, 2.9 Hz, 1H), 2.90 (t, J=11.9 Hz, 2H) 2.82 (d, J=11.3
Hz, 1H), 2.45 (dd, J=15.0, 6.7 Hz, 1H), 2.21-2.11 (m, 1H),
2.02-1.85 (m, 3H), 1.80-1.69 (m, 1H), 1.48 (s, 9H), 1.44-1.30 (m,
2H)
Step 11: The sulfate tetrabutylammonium salt
##STR00054##
[0349] tert-Butyl
4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}ami-
no)piperidine-1-carboxylate, (3.0 kg), THF (30 L), 2-picoline (1.61
L) and pyridine-SO.sub.3 complex (4.54 kg) were charged to a flask
under nitrogen. No exotherm was observed. The heterogeneous mixture
was allowed to stir overnight (.about.15 h). DCM (8 L) was then
added and the mixture was concentrated by vacuum distillation,
removing .about.30 L of THF/DCM. Additional DCM (28 L) was added,
followed by the addition of water (20 L). The flask was placed in
an ice bath and K.sub.2HPO.sub.4 (2.20 kg) was added over 4
minutes, followed by a water rinse (1 L). Bu.sub.4NHSO.sub.4 (2.90
kg) was then added over 10 minutes followed by additional water (4
L). The biphasic mixture was stirred for 30 minutes, after which
the bottom organic layer was transferred to a 100-L extractor via
an in-line filter. The aqueous layer remaining in the flask was
rinsed with additional DCM (2.times.4 L), and then also transferred
to the extractor. A small amount of aqueous layer (.about.2 L) had
also been transferred, and the two layers were separated. The
organic layer was returned to the extractor and washed with water
(1.times.6 L); pH was 4.5. The organic layer was separated and
charged to a new flask via an in-line filter. The mixture was
solvent-switched to 2,2,2-trifluoroethanol by vacuum distillation
(34 L final volume) and used as is in the next step. Water content
by Karl-Fisher titration was 1900 ppm.
[0350] In a smaller-scale experiment using the same procedure,
evaporation of the solvent gave a solid from which .sup.1H NMR data
were collected. .sup.1H NMR (400 MHz, CDCl.sub.3): 6.65 (d, J=8.4
Hz, 1H), 4.37-4.32 (m, 1H), 4.18-4.00 (m, 2H), 4.00-3.89 (m, 1H),
3.87 (d, J=7.7 Hz, 1H), 3.36-3.27 (m, 9H), 2.95-2.79 (m, 2H), 2.75
(d, J=11.4 Hz, 1H), 2.42 (dd, J=15.0, 6.9 Hz, 1H) 2.24-2.11 (m,
2H), 1.96-1.81 (m, 3H), 1.74-1.60 (m, 8H), 1.47 (s, 9H), 1.46 (m,
8H), 1.39 (m, 2H), 1.01 (t, J=7.3, 12H)
Step 12:
(2S,5R)-7-Oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide
##STR00055##
[0352] The solution of Bu.sub.4N.sup.+ -OSO.sub.3 salt in TFE (34
L) was used as received from the prior step with an assumed yield
of 100%. The reaction mixture was cooled in an ice bath, and
HBF.sub.4.Et.sub.2O (1.57 L) was added via addition funnel over 11
minutes between 18.degree. C. and 22.degree. C. The resulting white
slurry was allowed to stir overnight (12 hours). TFE (.about.15 L)
was removed by vacuum distillation. DCM (15 L) was then added. To a
100-L extractor was charged pyrogen-free water (35 L) and
NaHCO.sub.3 (274 g), and the solution was cooled to 13.degree. C.
The reaction mixture was transferred by vacuum into the extractor
with temperature of 11-13.degree. C. The reaction flask was rinsed
with additional DCM (5 L) and the suspension also transferred to
the extractor. The reaction mixture was warmed to 18.5.degree. C.
and de-pyrogenated water (12 L) was added to solubilize all the
solids. The final pH was 4.5. The organic layer was separated, and
the aqueous layer was washed with DCM (2.times.16 L). Assay of the
aqueous layer showed 2.38 kg (83.8%)
[0353] The aqueous layer was charged to a clean flask. The solution
was concentrated by vacuum distillation followed by azeotropic
distillation with IPA. At this time, .sup.1H NMR analysis of the
IPA:H.sub.2O ratio indicated the presence of 13.4 L of water and
24.6 L of IPA. IPA (22 L) was added. The white crystalline solid
was filtered and washed with 7:1 IPA:de-pyrogenated water (16 L)
and dried under vacuum and nitrogen at room temperature to afford
the title product in the form of a crystalline channel hydrate, 1.5
wt % water. (Yield=1.715 kg, 57.4% over Steps 11 and 12). .sup.1H
NMR (400 MHz, DMSO-d.sub.6): 8.3 (br s, 2H), 8.21 (d, J=7.8 Hz,
1H), 4.01 (s, 1H), 3.97-3.85 (m, 1H), 3.75 (d, J=6.5 Hz, 1H) 3.28
(dd, J=12.9, 2.5 Hz, 2H) 3.05-2.93 (m, 4H), 2.08-1.97 (m, 1H),
1.95-1.79 (m, 3H), 1.75-1.59 (m, 4H)
Example 1D
Crystalline monohydrate of
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide
Part A: Preparation
Example 1D
Crystalline monohydrate of
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide
Part A: Preparation
[0354] Amorphous
(2S,5R)-7-oxo-N-piperidin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-
-2-carboxamide (1 g) and deionized water (5 mL) were added to a
glass vial, and the resulting slurry was stirred at room
temperature until XRPD monitoring (see Part B) indicated that
conversion to a different form was complete. The crystalline solid
was then collected by gravity filtration and dried at room
temperature.
[0355] The crystalline slurry can alternatively be isolated by
evaporative removal which, because the crystalline hydrate is
soluble in water (about 55 g/mL at room temperature), can result in
a higher yield.
[0356] Drying can dehydrate the crystals, and thus drying methods
employing vacuums and/or high temperatures should generally not be
used. Controlling the relative humidity of the drying environment
can minimize or avoid dehydration. For example, the crystals can be
dried using a nitrogen steam with a controlled moisture content
(e.g., in a range of from about 40% to about 70% relative humidity)
to avoid dehydration.
[0357] The crystalline hydrate can also be obtained by slurrying in
a mixture of isopropyl alcohol and water and using any of the
isolation procedures set forth above. The ratio of isopropyl
alcohol to water is suitably about 7:1 by volume.
Part B: Characterization
[0358] An XRPD pattern of a crystalline monohydrate prepared in
accordance with the method described in Part A was generated on a
Philips Panalytical X'Pert Pro X-ray powder diffractometer with a
PW3040/60 console using a continuous scan from 4 to 40 degrees
2.THETA.. Copper K-Alpha 1 (K.alpha.1) and K-Alpha 2 (K.alpha.2)
radiation was used as the source. The experiment was conducted with
the sample at room temperature and open to the atmosphere. The XRPD
pattern is shown in FIG. 1. 2.THETA. values and the corresponding
d-spacings in the XRPD pattern include the following:
TABLE-US-00001 TABLE 1 XRPD of crystalline monohydrate Peak No.
d-spacing (.ANG.) 2 Theta 1 6.5 13.5 2 5.7 15.5 3 5.6 15.6 4 5.1
17.4 5 4.7 18.7 6 4.5 19.7 7 4.4 20.4 8 4.1 21.7 9 3.8 22.6 10 3.7
24.0 11 3.6 24.3 12 3.4 25.9 13 3.3 26.3 14 3.3 26.6 15 3.2 27.0 16
3.2 27.5 17 3.0 29.3 18 2.9 30.0 19 2.8 31.3 20 2.7 32.4 21 2.7
32.9 22 2.6 33.1 23 2.6 34.0 24 2.5 34.7 25 2.5 35.5 26 2.3
38.9
[0359] Crystalline monohydrate prepared in accordance with the
method described in Part A was analyzed with a TA Instruments DSC Q
1000 differential scanning calorimeter (DSC) at a heating rate of
10.degree. C./minute from 25.degree. C. to 350.degree. C. in an
open aluminum pan in a nitrogen atmosphere. The DSC curve (see FIG.
2) exhibited an endotherm due to water loss with an onset
temperature of 22.5.degree. C. and enthalpy change of 186 J/g.
Decomposition is observed above 270.degree. C.
[0360] A thermogravimetric analysis (TGA) of crystalline
monohydrate prepared in accordance with the method described in
Part A was performed with a TA Instruments TGA Q 500 under nitrogen
at a heating rate of 10.degree. C./minute from 25.degree. C. to
300.degree. C. The TGA showed a weight loss of 4.9 wt. % up to
100.degree. C. followed by decomposition above 270.degree. C. The
4.9 wt. % loss corresponds to the loss of 1 mole of water per mole
of the compound which is consistent with a monohydrate.
Example 2
(2S,5R)--N-[(4S)-Azepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]-o-
ctane-2-carboxamide
##STR00056##
[0361] Step 1: tert-Butyl
(4S)-4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]car-
bonyl}amino)azepane-1-carboxylate
[0362] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (51.7 mg, 0.187 mmol) in dry dichloromethane (2 mL) was
added a solution of tert-butyl (4S)-4-aminoazepane-1-carboxylate
(69 mg, 0.275 mmol), triethylamine (0.090 mL, 0.646 mmol), HOBT
(42.5 mg, 0.278 mmol), and EDC (54.7 mg, 0.285 mmol) sequentially
at room temperature under nitrogen. The reaction was stirred at
room temperature overnight. The reaction mixture was concentrated
under vacuum and the residue was purified by HPLC on a 30.times.100
mm Waters Sunfire column eluted with 15% to 100% CH.sub.3CN+0.05%
TFA/water+0.05% TFA over 15 minutes to afford the title compound as
a white solid after lyophilization.
Step 2: tert-Butyl
(4S)-4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbony-
l}amino)azepane-1-carboxylate
[0363] Palladium on carbon (11.8 mg; 10% Pd/C) was added to a
solution of tert-butyl
(4S)-4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]car-
bonyl}amino)azepane-1-carboxylate (49.7 mg, 0.33 mmol) in methanol
(1.5 mL) and the resulting mixture was stirred under hydrogen
(balloon) for 3 hours. TLC analysis showed the reaction was
complete. The reaction mixture was filtered through a microfilter
and the filtrate was concentrated under vacuum to afford the impure
title compound as a white foam (44.8 mg) which was used without
purification in the next step.
Step 3: N,N,N-Tributylbutan-1-aminium
{[((2S,5R)-2-{[(4S)-azepan-4-ylamino]carbonyl}-7-oxo-1,6-diazabicyclo[3.2-
.1]oct-6-yl)oxy]sulfonyl}oxidanide
[0364] To a solution of impure tert-butyl
(4S)-4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbony-
l}amino)azepane-1-carboxylate (40.2 mg, 0.105 mmol) in pyridine (1
mL) was added sulfur trioxide pyridine complex (42.1 mg, 0.265
mmol). The mixture was stirred at room temperature under nitrogen
overnight. LC/MS analysis showed incomplete reaction.). Additional
pyridine was added followed by additional sulfur trioxide pyridine
complex (40 mg). The resulting mixture was stirred at room
temperature for five hours. The reaction was filtered and the
solids were washed with dichloromethane. The filtrate was
concentrated in vacuo and suspended in saturated aqueous potassium
dihydrogenphosphate solution. The resulting mixture was extracted
with ethyl acetate. The aqueous layer was collected and
tetrabutylammonium hydrogen sulfate (0.036 mg, 0.105 mmol) was
added. The mixture was stirred for 10 minutes then extracted with
EtOAc (4.times.). The organic layers were combined, dried over
sodium sulfate, and concentrated in vacuo to afford the title
compound as a colorless oil.
Step 4:
(2S,5R)--N-[(4S)-Azepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[-
3.2.1]octane-2-carboxamide
[0365] To a solution of N,N,N-tributylbutan-1-aminium
{[((2S,5R)-2-{[(4S)-azepan-4-ylamino]carbonyl}-7-oxo-1,6-diazabicyclo[3.2-
.1]oct-6-yl)oxy]sulfonyl}oxidanide (30.9 mg, 0.067 mmol) in
anhydrous dichloromethane (7 mL) at 0.degree. C. under nitrogen was
added trifluoroacetic acid (0.5 mL, 6.5 mmol) dropwise. The
reaction mixture was stirred for two hours then concentrated under
vacuum. Ether was added to the residue and the resulting white
precipitate was collected by centrifugation. The precipitate was
purified by HPLC on a Phenomenex Synergy Polar-RP 80A column and
lyophilized to afford the title compound as a white solid. LC-MS
(negative ionization) m/e 361 (M-H); LC-MS (positive ionization)
m/e 385 (M+Na); .sup.1H NMR (600 MHz, D.sub.2O; unreferenced)
(.delta., ppm) 4.17 (1H, br d, J=3 Hz), 3.96-4.03 (2H, m),
3.27-3.38 (3H, m), 3.15-3.22 (2H, m), 3.02 (1H, d, J=12 Hz),
1.62-2.18 (m, 12H).
Example 3
(2S,5R)--N-[(4R)-Azepan-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oc-
tane-2-carboxamide
##STR00057##
[0367] By substituting tert-butyl (4R)-4-aminoazepane-1-carboxylate
for tert-butyl (4S)-4-aminoazepane-1-carboxylate in the procedure
of Example 2, the title compound can be prepared.
Example 4
(2S,5R)-7-Oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.1-
]-octane-2-carboxamide
##STR00058##
[0368] Step 1: tert-Butyl
(3R)-3-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]car-
bonyl}amino)pyrrolidine-1-carboxylate
[0369] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (53 mg, 0.192 mmol) in dry dichloromethane (2 mL) was added
a solution of tert-butyl (3R)-3-aminopyrrolidine-1-carboxylate (55
mg, 0.288 mmol), triethylamine (0.061 mL, 0.441 mmol), HOBT (44.1
mg, 0.288 mmol), and EDC (55.2 mg, 0.288 mmol) sequentially at room
temperature under nitrogen. The reaction was stirred at room
temperature for six hours. The reaction mixture was concentrated
under vacuum and the residue was purified by HPLC on a 30.times.100
mm Waters Sunfire column eluted with 15% to 100% CH3CN+0.05%
TFA/water+0.05% TFA over 15 minutes to afford the title
compound.
Step 2: tert-Butyl
(3R)-3-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbony-
l}amino)pyrrolidine-1-carboxylate
[0370] Palladium on carbon (9.18 mg; 10% Pd/C) was added to a
solution of tert-butyl
(3R)-3-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]car-
bonyl}amino)pyrrolidine-1-carboxylate (38 mg, 0.085 mmol) in
methanol (2 mL) and the resulting mixture was stirred under
hydrogen (balloon) for 3 hours. TLC analysis showed the reaction
was complete. The reaction mixture was filtered through a
microfilter and the filtrate was concentrated under vacuum to
afford the impure title compound as an oil.
Step 3: N,N-Dibutylbutan-1-aminium
({[(2S,5R)-2-({[(3R)-1-(tert-butoxycarbonyl)pyrrolidin-3-yl]amino}carbony-
l)-7-oxo-1,6-diazabicyclo[3.2.1]oct-6-yl]oxy}sulfonyl)oxidanide
[0371] To a solution of tert-butyl
(3R)-3-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbony-
l}amino)pyrrolidine-1-carboxylate (30 mg, 0.085 mmol) in pyridine
(1 mL) was added sulfur trioxide pyridine complex (53.9 mg, 0.339
mmol) and 4 A molecular sieves. The mixture was stirred at room
temperature under nitrogen overnight. LC/MS analysis showed
incomplete reaction. The reaction mixture was filtered and the
filtrate was concentrated under vacuum. The residue was
chromatographed on HPLC to recover unreacted starting material
which was resubjected to the reaction conditions. The combined
product was suspended in saturated aqueous potassium
dihydrogenphosphate solution. The resulting mixture was washed with
ethyl acetate. The aqueous layer was collected and
tetrabutylammonium hydrogen sulfate was added. The mixture was
stirred for 10 minutes then extracted with EtOAc (4.times.). The
organic layers were combined, dried over sodium sulfate, and
concentrated in vacuo to afford the title compound as a colorless
oil.
Step 4:
(2S,5R)-7-Oxo-n-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyc-
lo[3.2.1]-octane-2-carboxamide
[0372] To a solution of N,N-dibutylbutan-1-aminium
({[(2S,5R)-2-({[(3R)-1-(tert-butoxycarbonyl)pyrrolidin-3-yl]amino}carbony-
l)-7-oxo-1,6-diazabicyclo[3.2.1]oct-6-yl]oxy}sulfonyl)oxidanide (2
mg, 0.046 mmol) in anhydrous dichloromethane (0.5 mL) at 0.degree.
C. under nitrogen was added trifluoroacetic acid (0.525 mL, 0.046
mmol) dropwise. The reaction mixture was stirred for two hours then
concentrated under vacuum. Ether was added to the residue and the
resulting white precipitate was collected by centrifugation. The
purified by HPLC on a Phenomenex Synergy Polar-RP 80A column and
lyophilized to afford the title compound as a white solid. LC-MS
(negative ionization) m/e 333 (M-H); LC-MS (positive ionization)
m/e 336 (M+H); .sup.1H NMR (600 MHz, D.sub.2O; unreferenced)
(.delta., ppm) 4.50-4.54 (1H, m), 4.20 (1H, dd, J=3, 6 Hz), 4.03
(1H, br d, H=7 Hz), 3.54 (1H, dd, J=7, 13 Hz), 3.40-3.48 (1H, m),
3.30-3.35 (2H, m), 3.24 (1H, dd, J=5, 13 Hz), 3.07 (1H, d, J=12
Hz), 2.31-2.37 (1H, m), 2.15-2.20 (1H, m), 2.00-2.10 (2H, m),
1.88-1.98 (1H, m), 1.76-1.84 (1H, m).
Example 5
(2S,5R)--N-Azocan-5-yl-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2--
carboxamide
##STR00059##
[0374] By substituting tert-butyl 5-aminoazocane-1-carboxylate for
4-amino-1-BOC-piperidine in the procedure of Example 1, the title
compound can be prepared.
Example 6
(2S,5R)-7-Oxo-N-pyridin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]octane-2--
carboxamide
##STR00060##
[0375] Step 1:
(2S,5R)-6-(Benzyloxy)-7-oxo-N-pyridin-4-yl-1,6-diazabicyclo[3.2.1]octane--
2-carboxamide
[0376] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (51.5 mg, 0.186 mmol) in dry dichloromethane (5 mL) was
added triethylamine (0.065 mL, 0.466 mmol),
2-chloro-1-methylpyridinium iodide (63.4 mg, 0.248 mmol), and
4-aminopyridine (19.2 mg, 0.204 mmol) sequentially at room
temperature under nitrogen. The reaction was then heated to
50.degree. C. for 1.5 hours. LC/MS showed reaction complete. The
reaction mixture was concentrated and purified by HPLC on a
30.times.100 mm Waters Sunfire column to afford the title compound
as an orange solid after lyophilization.
Step 2:
(2S,5R)-6-hydroxy-7-oxo-N-pyridin-4-yl-1,6-diazabicyclo[3.2.1]octa-
ne-2-carboxamide
[0377] Palladium on carbon (13.2 mg; 10% Pd/C) was added to a
solution of
(2S,5R)-6-(benzyloxy)-7-oxo-N-pyridin-4-yl-1,6-diazabicyclo[3.2.1]octane--
2-carboxamide (52.7 mg, 0.15 mmol; combined product of two runs) in
methanol (1.5 mL) and the resulting mixture was stirred under
hydrogen (balloon) for 5 hours. TLC and HPLC analysis showed a
small amount of starting material remaining. Additional catalyst
(5.6 mg) was added and the resulting mixture was stirred under
hydrogen (balloon) for an additional 1 hour. The reaction mixture
was filtered through a microfilter and the filtrate was
concentrated under vacuum to afford the title compound as a
colorless oil.
Step 3:
(2S,5R)-7-Oxo-N-pyridin-4-yl-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]o-
ctane-2-carboxamide
[0378] To a solution of
(2S,5R)-6-hydroxy-7-oxo-N-pyridin-4-yl-1,6-diazabicyclo[3.2.1]octane-2-ca-
rboxamide (9.2 mg, 0.035 mmol) in dry pyridine (0.5 mL) was added
dried 4 A molecular sieves and sulfur trioxide pyridine complex (22
mg, 0.138 mmol) at room temperature under nitrogen. The mixture was
stirred for four hours. The reaction mixture was filtered and the
solids washed with dichloromethane, acetonitrile, and methanol. The
filtrate was concentrated under vacuum and the residue was
triturated with ethyl acetate. The residue was dried under vacuum,
dissolved in saturated sodium dihydrogen phosphate and purified by
HPLC on a Phenomenex Synergi Polar-RP 80A column to afford the
title compound as a white solid after lyophilization. LC-MS
(negative ionization) m/e 341 (M-H); LC-MS (positive ionization)
m/e 343 (M+H); .sup.1H NMR (600 MHz, D.sub.2O; unreferenced)
(.delta., ppm) 8.57 (2H, br s), 8.15 (2H, br s), 4.27 (1H, br d,
J=7 Hz), 4.20 (1H, br s), 3.33 (1H, d, J=12 Hz), 3.10 (1H, d, J=12
Hz), 2.28-2.32 (1H, m), 2.08-2.11 (1H, m), 1.93-1.98 (1H, m),
1.83-1.88 (1H, m).
Example 7
(2S,5R)--N-(2-Methoxypyridin-4-yl)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxamide
##STR00061##
[0379] Step 1:
(2S,5R)-6-(Benzyloxy)-N-(2-methoxypyridin-4-yl)-7-oxo-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide
[0380] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (38.9 mg, 0.141 mmol) in dry dichloromethane (2 mL) was
added triethylamine (0.049 mL, 0.352 mmol),
2-chloro-1-methylpyridinium iodide (53.3 mg, 0.209 mmol), and
2-methoxy-4-aminopyridine (20.2 mg, 0.163 mmol) sequentially at
room temperature under nitrogen. The reaction was then heated to
50.degree. C. for 1.5 hours. LC/MS showed reaction complete. The
reaction mixture was concentrated and purified by HPLC on a
30.times.100 mm Waters Sunfire column to afford the title compound
as an orange solid after lyophilization.
Step 2:
(2S,5R)-6-Hydroxy-N-(2-methoxypyridin-4-yl)-7-oxo-1,6-diazabicyclo-
[3.2.1]octane-2-carboxamide
[0381] Palladium on carbon (13.4 mg; 10% Pd/C) was added to a
solution of
(2S,5R)-6-(benzyloxy)-N-(2-methoxypyridin-4-yl)-7-oxo-1,6-diazabicyclo[3.-
2.1]octane-2-carboxamide (43.6 mg, 0.114 mmol) in methanol (1 mL)
and the resulting mixture was stirred under hydrogen (balloon)
overnight. HPLC analysis showed reaction complete. The reaction
mixture was filtered through a microfilter and the filtrate was
concentrated under vacuum to afford the title compound as an impure
colorless oil.
Step 3:
(2S,5R)--N-(2-Methoxypyridin-4-yl)-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide
[0382] To a solution of
(2S,5R)-6-hydroxy-N-(2-methoxypyridin-4-yl)-7-oxo-1,6-diazabicyclo[3.2.1]-
octane-2-carboxamide (33 mg, 0.114 mmol) in dry pyridine (1 mL) was
added sulfur trioxide pyridine complex (111 mg, 0.696 mmol) at room
temperature under nitrogen. The mixture was stirred for four hours.
The reaction mixture was filtered and the solids washed with
dichloromethane, acetonitrile, and methanol. The filtrate was
concentrated under vacuum and the residue was triturated with ethyl
acetate then dried under vacuum, dissolved in saturated sodium
dihydrogen phosphate and purified by HPLC on a Phenomenex Synergi
Polar-RP 80A column to give a white solid which was further
purified on a Waters Sunfire column to give a white solid which was
further purified by HPLC on a Phenomenex Synergi Polar-RP 80A
column to afford the title compound as a white solid after
lyophilization. LC-MS (negative ionization) m/e 371 (M-H); LC-MS
(positive ionization) m/e 373 (M+H); .sup.1H NMR (600 MHz,
D.sub.2O; unreferenced) (.delta., ppm) 8.10 (1H, br d, J=6 Hz),
7.59 (1H, s), 7.41 (1H, d, J=6 Hz), 4.26 (1H, br d, J=7 Hz), 4.23
(1H, br s), 4.07 (3H, s), 3.36 (1H, d, J=12 Hz), 3.12 (1H, d, J=12
Hz), 2.29-2.33 (1H, m), 2.10-2.14 (1H, m), 1.93-1.99 (1H, m),
1.84-1.90 (1H, m).
Example 8
(2S,5R)--N-[2-(Dimethylamino)pyridin-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide
##STR00062##
[0383] Step 1:
(2S,5R)-6-(Benzyloxy)-N-[2-(dimethylamino)pyridin-4-yl]-7-oxo-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide
[0384] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (37.3 mg, 0.135 mmol) in dry dichloromethane (2.5 mL) was
added triethylamine (0.047 mL, 0.338 mmol),
2-chloro-1-methylpyridinium iodide (38.3 mg, 0.15 mmol), and
2-dimethylamino-4-aminopyridine (21.7 mg, 0.158 mmol) sequentially
at room temperature under nitrogen. The reaction was then heated to
50.degree. C. for 1.5 hours. LC/MS showed reaction complete. The
reaction mixture was concentrated and purified by HPLC on a
30.times.100 mm Waters Sunfire column to afford the title compound
as an off-white solid after lyophilization.
Step 2:
(2S,5R)--N-[2-(Dimethylamino)pyridin-4-yl]-6-hydroxy-7-oxo-1,6-dia-
zabicyclo[3.2.1]octane-2-carboxamide
[0385] Palladium on carbon (10.4 mg; 10% Pd/C) was added to a
solution of
(2S,5R)-6-(benzyloxy)-N-[2-(dimethylamino)pyridin-4-yl]-7-oxo-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide (47.8 mg, 0.121 mmol) in methanol
(2 mL) and the resulting mixture was stirred under hydrogen
(balloon) overnight. HPLC analysis showed reaction complete. The
reaction mixture was filtered through a microfilter and the
filtrate was concentrated under vacuum to afford the title compound
as a colorless oil which was used in the next step without further
purification.
Step 3:
(2S,5R)--N-[2-(Dimethylamino)pyridin-4-yl]-7-oxo-6-(sulfooxy)-1,6--
diazabicyclo[3.2.1]octane-2-carboxamide
[0386] To a solution of
(2S,5R)--N-[2-(dimethylamino)pyridin-4-yl]-6-hydroxy-7-oxo-1,6-diazabicyc-
lo[3.2.1]octane-2-carboxamide (37 mg, 0.121 mmol) in dry pyridine
(1.5 mL) was added sulfur trioxide pyridine complex (92 mg, 0.578
mmol) at room temperature under nitrogen. The mixture was stirred
for seven hours. NMR analysis of an aliquot showed incomplete
reaction. Additional pyridine (2 mL) and sulfur trioxide pyridine
complex (60 mg) were added and the resulting mixture was stirred at
room temperature under nitrogen. The reaction mixture was
concentrated under vacuum and the residue was purified by HPLC on a
Phenomenex Synergi Polar-RP 80A column to give impure product which
was further purified on a Waters Sunfire column to afford the title
compound as a white solid after lyophilization. LC-MS (negative
ionization) m/e 384 (M-H); LC-MS (positive ionization) m/e 386
(M+H); .sup.1H NMR (600 MHz, D.sub.2O; unreferenced) (.delta., ppm)
7.77 (1H, br d, J=6 Hz), 7.41 (1H, s), 6.94 (1H, d, J=6 Hz), 4.23
(2H, br s), 3.36 (1H, d, J=12 Hz), 3.18 (6H, s), 3.11 (1H, d, J=12
Hz), 2.29-2.32 (1H, m), 2.10-2.14 (1H, m), 1.93-1.99 (1H, m),
1.84-1.90 (1H, m).
Example 9
(2S,5R)--N-[4-(Aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxamide
##STR00063##
[0387] Step 1:
tert-butyl[4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2--
yl]carbonyl}amino)benzyl]carbamate
[0388] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (29.9 mg, 0.108 mmol) in dry dichloromethane (3 mL) was
added triethylamine (0.038 mL, 0.271 mmol),
2-chloro-1-methylpyridinium iodide (41.0 mg, 0.160 mmol), and
4-(N-BOC-aminomethyl)aniline (30.6 mg, 0.138 mmol) sequentially at
room temperature under nitrogen. The reaction was then heated to
60.degree. C. for 2 hours. LC/MS showed no starting material
remaining. The reaction mixture was concentrated and purified by
HPLC on a Waters Sunfire column to afford the title compound.
Step 2:
tert-butyl[4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-
-2-yl]carbonyl}amino)benzyl]carbamate
[0389] Palladium on carbon (7.8 mg; 10% Pd/C) was added to a
solution of the product of step 1 (35 mg, 0.073 mmol) in methanol
(2 mL), and the resulting mixture was stirred under hydrogen
(balloon) overnight. LC-MS analysis showed the reaction was
complete. The reaction mixture was filtered through a microfilter
and the filtrate was concentrated under vacuum which was azeotroped
from toluene to afford the title compound as a white solid.
Step 3:
tert-butyl[4-({[(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]-
oct-2-yl]carbonyl}amino)benzyl]carbamate
[0390] To a solution of the product of step 2 (28.9 mg, 0.074 mmol)
in dry pyridine (1 mL) was added sulfur trioxide pyridine complex
(60.3 mg, 0.379 mmol) at room temperature under nitrogen. The
mixture was stirred at room temperature overnight. LC-MS analysis
showed reaction approximately 50% complete. Additional sulfur
trioxide pyridine complex (64.4 mg) was added to the reaction
mixture and stirring continued at room temperature. After 7 hours,
LC-MS analysis showed mostly product. The reaction mixture was
filtered and the insoluble solids were washed well with
dichloromethane. The filtrate was concentrated under vacuum and the
residue was azeotroped with toluene to remove excess pyridine. The
crude title compound thus obtained was used without further
purification in the next reaction.
Step 4:
(2S,5R)--N-[4-(aminomethyl)phenyl]-7-oxo-6-(sulfooxy)-1,6-diazabic-
yclo[3.2.1]octane-2-carboxamide
[0391] To a mixture of the product of step 3 (34.8 mg, 0.074 mmol)
in dry dichloromethane (3 mL) at 0.degree. C. under nitrogen was
added trifluoroacetic acid (1.0 mL, 13 mmol). All solids dissolved
immediately upon addition of trifluoroacetic acid. the solution was
stirred for 1.5 ours at which time LC-MS analysis showed reaction
complete. The reaction was concentrated under vacuum and the
residue was triturated with ether to remove excess trifluoroacetic
acid and organic impurities. The resulting sticky solid was dried
under vacuum and stored in the freezer overnight. The crude product
was purified by HPLC on a Phenomenex Synergi Polar-RP 80A column to
afford the impure title compound as a white solid after
lyophilization. The solid was triturated with acetonitrile
(3.times.) to afford the pure title compound as a white solid.
LC-MS (negative ionization) m/e 369 (M-H); LC-MS (positive
ionization) m/e 354 (M+H-NH.sub.3); .sup.1H NMR (600 MHz, D.sub.2O;
unreferenced) (.delta., ppm) 7.53 (2H, d, J=8.5 Hz), 7.46 (2H, d,
J=8.5 Hz), 4.23 (2H, br s), 4.11 (2H, s), 3.39 (1H, d, J=12 Hz),
3.18 (1H, d, J=12 Hz), 2.27-2.31 (1H, m), 2.09-2.14 (1H, m),
1.90-2.00 (1H, m), 1.813-1.89 (1H, m).
Example 10
(2S,5R)-7-Oxo-2-[(piperidin-4-ylamino)carbonyl]-1,6-diazabicyclo[3.2.1]oct-
ane-6-sulfonic acid
##STR00064##
[0392] Step 1: tert-Butyl
4-[({(2S,5R)-7-oxo-6-[(phenoxycarbonothioyl)oxy]-1,6-diazabicyclo[3.2.1]o-
ct-2-yl}carbonyl)amino]piperidine-1-carboxylate
[0393] A solution phenyl chlorothionocarbonate (1.25 eq.) in
dichloromethane is added to a solution of pyridine (1.25 eq.),
4-dimethylaminopyridine (0.1 eq.) and tert-butyl
4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}ami-
no)piperidine-1-carboxylate (see Example 1, Step 2) in
dichloromethane. The resulting mixture is stirred at room
temperature overnight then cooled in an ice bath and quenched by
addition of water. The layers are separated and the aqueous layer
is extracted with dichloromethane. The combined organic layers are
dried over sodium sulfate, filtered, and concentrated under vacuum.
The residue is purified by silica gel chromatography to afford the
title compound.
Step 2: tert-Butyl
4-({[(2S,5R)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbonyl}amino)-piperi-
dine-1-carboxylate
[0394] AIBN (0.1 eq.) is added to a solution of tert-butyl
4-[({(2S,5R)-7-oxo-6-[(phenoxycarbonothioyl)oxy]-1,6-diazabicyclo[3.2.1]o-
ct-2-yl}carbonyl)amino]piperidine-1-carboxylate in dry benzene and
the resulting mixture is heated to reflux. A solution of
tributyltin hydride (1.25 eq.) in benzene is added over a period of
one hour and the resulting mixture is refluxed for an additional 3
hours. The reaction mixture is concentrated under vacuum and the
residue is purified by silica gel chromatography to afford the
title compound.
Step 3:
(2S,5R)-2-({[1-(tert-Butoxycarbonyl)piperidin-4-yl]amino}carbonyl)-
-7-oxo-1,6-diazabicyclo[3.2.1]octane-6-sulfonic acid
[0395] The product of step 2 is sulfated according to the procedure
of Step 3 of Example 1 to afford the title compound.
Step 4:
(2S,5R)-7-oxo-2-[(piperidin-4-ylamino)carbonyl]-1,6-diazabicyclo[3-
.2.1]octane-6-sulfonic acid
[0396]
(2S,5R)-2-({[1-(tert-Butoxycarbonyl)piperidin-4-yl]amino}carbonyl)--
7-oxo-1,6-diazabicyclo[3.2.1]octane-6-sulfonic acid is deprotected
according to the procedure of Step 4 of Example 1 to afford the
title compound.
Example 11
(4R,65)-2-Oxo-N-piperidin-4-yl-3-(sulfooxy)-1,3-diazabicyclo[2.2.1]heptane-
-6-carboxamide
##STR00065##
[0398] By substituting
(4R,6S)-3-(benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carboxylic
acid for
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2--
carboxylic acid in the procedure of Example 1, the title compound
can be prepared.
Example 12
(4R,6S)-2-Oxo-N-[(4S)-azepan-4-yl]-3-(sulfooxy)-1,3-diazabicyclo[2.2.1]hep-
tane-6-carboxamide
##STR00066##
[0400] By substituting
(4R,6S)-3-(benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carboxylic
acid for
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2--
carboxylic acid in the procedure of Example 2, the title compound
can be prepared.
Example 13
(4R,6S)-2-Oxo-N-pyridin-4-yl-3-(sulfooxy)-1,3-diazabicyclo[2.2.1]heptane-6-
-carboxamide
##STR00067##
[0402] By substituting
(4R,6S)-3-(benzyloxy)-2-oxo-1,3-diazabicyclo[2.2.1]heptane-6-carboxylic
acid for
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2--
carboxylic acid in the procedure of Example 6, the title compound
can be prepared.
Example 14
(2S,5R)-7-Oxo-N-[(3R)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyclo[3.2.1-
]octane-2-carboxamide
##STR00068##
[0403] Step 1: tert-butyl
(3S)-3-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]car-
bonyl}amino)pyrrolidine-1-carboxylate
[0404] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (1 g, 3.62 mmol) in dry dichloromethane (30 mL) was added
dimethylaminopyridine (884 mgL, 7.24 mmol), EDC (1.388 g, 7.24
mmol), and tert-butyl (3S)-3-aminopyrrolidine-1-carboxylate (742
mg, 3.98 mmol) sequentially at room temperature under nitrogen. The
reaction mixture was stirred at room temperature over the weekend.
The reaction mixture was then concentrated under vacuum and the
residue was purified by HPLC on a 30.times.100 mm Waters Sunfire
column eluted with 15% to 100% CH.sub.3CN+0.05% TFA/water+0.05% TFA
over 15 minutes to afford the title compound as a white solid.
Step 2: tert-butyl
(3S)-3-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]carbony-
l}amino)pyrrolidine-1-carboxylate
[0405] Palladium on carbon (335 mg; 10% Pd/C) was added to a
solution of the product of Step 1 (1.4 g, 3.15 mmol) in methanol
(30 mL) and the resulting mixture was stirred under hydrogen
(balloon) for 1 hour. LC-MS analysis showed the reaction was
complete. The reaction mixture was filtered and the filtrate was
concentrated under vacuum to afford the title compound as an oil
which was used without purification in the next step.
Step 3:
tert-butyl3-({[(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]o-
ct-2-yl]carbonyl}amino)pyrrolidine-1-carboxylate
[0406] To a solution of the product of Step 2 (1.11 g, 3.15 mmol,
theoretical yield of Step 2) in pyridine (10 mL) was added sulfur
trioxide pyridine complex (2.51 g, 15.75 mmol). The mixture was
stirred at room temperature under nitrogen overnight.
Dichloromethane was added and the mixture was filtered. The
collected solid was washed with dichloromethane (4.times.) and the
combined filtrates were concentrated under vacuum. The residue was
used without purification in the next step.
Step 4:
(2S,5R)-7-oxo-N-[(3S)-pyrrolidin-3-yl]-6-(sulfooxy)-1,6-diazabicyc-
lo[3.2.1]-octane-2-carboxamide
[0407] To a solution of the product of Step 3 (1.37 g, 3.15 mmol,
theoretical yield of step 3) in anhydrous dichloromethane (5 mL) at
0.degree. C. under nitrogen was added TFA (2 mL, 26 mmol) dropwise.
The reaction mixture was stirred for two hours then concentrated
under vacuum. Ether was added to the residue and the resulting
white precipitate was collected by centrifugation (ether
trituration repeated two more times). The resulting solid was
purified by HPLC on a Phenomenex Synergy Polar-RP 80A column eluted
with methanol/water and lyophilized to afford the title compound as
a white solid. LC-MS (negative ionization mode) m/e 333 (M-H).
LC-MS (positive ionization) m/e 335 (M+H), 357 (M+Na); .sup.1H NMR
(600 MHz, D.sub.2O; unreferenced) (.delta., ppm) 4.51 (1H, m), 4.16
(1H, br d, J=2.6 Hz), 3.99 (1H, d, J=7 Hz), 3.54 (1H, dd, J=7, 13
Hz), 3.40-3.50 (1H, m), 3.30-3.40 (1H, m), 3.20-3.30 (2H, m), 3.02
(1H, d, J=12 Hz), 2.30-2.40 (1H, m), 2.10-2.20 (1H, m), 2.00-2.10
(2H, m), 1.83-1.93 (1H, m), 1.72-1.80 (1H, m).
Example 15
(2S,5R)--N-[(3R,4S)-3-Fluoropiperidin-4-yl]-7-oxo-6-(sulfooxy)-1,6-diazabi-
cyclo[3.2.1]octane-2-carboxamide
##STR00069##
[0408] Step 1:
tert-butyl-(3R,4S)-4-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.-
1]oct-2-yl]carbonyl}amino)-3-fluoropiperidine-1-carboxylate
[0409] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (2.108 g, 7.63 mmol) in anhydrous dimethylformamide (15 mL)
was added BOP (4.05 g, 9.15 mmol) and the resulting mixture was
stirred at room temperature under nitrogen for 5 minutes.
Diisopropyl ethyl amine (2.66 mL, 15.26 mmol) was then added
followed by a solution of tert-butyl
(3R,4S)-4-amino-3-fluoropiperidine-1-carboxylate (1.665 g, 7.63
mmol) in 20 mL of dichloromethane. The resulting solution was
stirred at room temperature under nitrogen for 2 hours then
concentrated under vacuum and the residue partitioned between ethyl
acetate and water. The aqueous layer was washed twice with ethyl
acetate. The organic layer was washed with brine, dried over sodium
sulfate, and concentrated under vacuum. The residue was purified
via silica gel flash chromatography (Isco Combiflash apparatus--120
g silica gel, 80 mL/min, 254 nM, 0% to 100% EtOAc/hexane over 6
column volumes then 100% EtOAc for 9 column volumes; title compound
eluted at 100% EtOAc). Fractions containing pure title compound
were collected and concentrated in vacuo to give a tan solid.
Fractions containing impure product were also collected and
repurified by HPLC (30.times.100 mm Sunfire column, 5 microns, 35
mL/min, 10% to 100% CH3CN+0.1% TFA/water+0.1% TFA over 15 min.;
title compound eluted at 70% CH.sub.3CN+0.1% TFA). Fractions
containing pure product were combined and concentrated in vacuo.
The resulting aqueous residue was then extracted with ethyl
acetate. The organic layer was collected and dried over magnesium
sulfate. Concentration in vacuo gave a white solid which was
combined with the material isolated from silica gel chromatography
to afford the title compound.
Step 2:
tert-butyl-(3R,4S)-4-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3-
.2.1]oct-2-yl]carbonyl}amino)-3-fluoropiperidine-1-carboxylate
[0410] To a solution of the product of Step 1 (3.0175 g, 6.33 mmol)
in methanol (80 mL) and ethyl acetate (20 mL), was added 10%
palladium on carbon (0.73 g, 6.86 mmol) and the reaction mixture
was stirred under an atmosphere of hydrogen (balloon) overnight.
LC/MS analysis showed reaction was complete. The reaction mixture
was filtered through a microfilter and the collected solid was
washed well with methanol. The filtrate was concentrated under
vacuum and azeotroped with toluene to afford the title compound as
a yellow foam which was used directly in the next step without
purification
Step 3:
tert-butyl-(3R,4S)-4-({[(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicycl-
o[3.2.1]oct-2-yl]carbonyl}amino)-3-fluoropiperidine-1-carboxylate
[0411] To a solution of the product of Step 2 (2.59 g, 6.7 mmol,
theoretical yield of Step 2) in pyridine (30 mL) was added sulfur
trioxide pyridine complex (5.40 g, 34 mmol). The mixture was
stirred at room temperature under nitrogen for 3 hours then
additional sulfur trioxide pyridine complex (5.40 g, 34 mmol) was
added and the reaction mixture was stirred at room temperature
overnight. Dichloromethane was then added and the mixture was
filtered. The collected solid was washed thoroughly with
dichloromethane and the combined filtrates were concentrated under
vacuum to afford the crude title compound. The residue was used
without purification in the next step.
Step 4:
(2S,5R)--N-[(3R,4S)-3-fluoropiperidin-4-yl]-7-oxo-6-(sulfooxy)-1,6-
-diaza bicyclo[3.2.1]octane-2-carboxamide
[0412] To a solution of the product of Step 3 (3.13 g, 6.7 mmol,
theoretical yield of Step 3) in anhydrous dichloromethane (50 mL)
at 0.degree. C. under nitrogen was added trifluoroacetic acid (10
mL, 130 mmol) dropwise. The reaction mixture was allowed to warm to
room temperature then stirred for two hours. Additional
trifluoroacetic acid (6 mL, 78 mmol) was added and the reaction
mixture was stirred at room temperature for an additional 3 hours
then concentrated under vacuum. Ether was added to the residue and
the resulting white precipitate was collected by centrifugation
(ether trituration repeated two more times). The resulting solid
was purified by HPLC on a Phenomenex Synergy Polar-RP 80A column
eluted with methanol/water and lyophilized to afford the title
compound as a cream colored solid which contained .about.6%
pyridine by NMR. This impure product was triturated and sonicated
twice with acetonitrile (solid isolated by centrifugation) to
afford the title compound as a white solid. LC-MS (negative
ionization mode) m/e 365 (M-H).
Example 16
(2S,5R)-7-Oxo-6-(sulfooxy)-N-(1,2,3,4-tetrahydroisoquinolin-6-yl)-1,6-diaz-
abicyclo[3.2.1]octane-2-carboxamide
##STR00070##
[0413] Step 1:
tert-butyl-6-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2--
yl]carbonyl}amino)-3,4-dihydroisoquinoline-2(1H)-carboxylate
[0414] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (53.3 mg, 0.193 mmol) in dry dichloromethane (2 mL) was
added triethylamine (0.067 mL, 0.482 mmol),
2-chloro-1-methylpyridinium iodide (58.7 mg, 0.230 mmol), and
6-amino-2-N-BOC-1,2,3,4-tetrahydro-isoquinoline (54.8 mg, 0.221
mmol) sequentially at room temperature under nitrogen. The reaction
mixture was then heated to 50.degree. C. for 45 minutes then the
reaction product was concentrated in vacuo. Attempts to dissolve
the reaction product in eluant (2:1:1 CH.sub.3CN/DMSO/water) for
HPLC were unsuccessful, so it was partitioned between aqueous layer
and dichloromethane. The organic layer was collected, dried over
sodium sulfate, concentrated in vacuo and set aside for separate
purification. The aqueous layer was also collected and purified by
HPLC (30.times.100 mm Waters Sunfire column; 5 micron; 35 mL/min.;
210 nM; 15% to 100% CH.sub.3CN+0.05% TFA/water+0.05% TFA over 15
minutes; the title compound eluted at 80% CH.sub.3CN+0.05%
TFA/water+0.05% TFA). Fractions containing the title compound were
lyophilized overnight to afford the title compound as a white
sticky solid. The organic layer from partitioning the crude product
was purified by preparative TLC (1000 micron silica gel plate
eluted with 50% ethyl acetate/hexane) to afford the title compound.
Both batches of the title compound were combined and used without
further purification in the next step.
Step 2:
tert-butyl-6-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-
-2-yl]carbonyl}amino)-3,4-dihydroisoquinoline-2(1H)-carboxylate
[0415] To a solution of the product of Step 1 (79.6 mg, 0.157 mmol)
in methanol (4 mL) and ethyl acetate (2 mL), was added 10%
palladium on carbon (18 mg) and the reaction mixture was stirred
under an atmosphere of hydrogen (balloon) overnight. LC/MS analysis
showed the reaction was not quite complete so an additional 10%
palladium on carbon (10 mg) was added and the reaction mixture was
stirred under an atmosphere of hydrogen (balloon) for an additional
6 hours. The reaction mixture was filtered through a microfilter
and the collected solid was washed well with methanol. The filtrate
was concentrated under vacuum and azeotroped with toluene to afford
the title compound as a light brown oil which was used directly in
the next step without purification
Step 3:
tert-butyl-6-({[(2S,5R)-7-oxo-6-sulfooxy-1,6-diazabicyclo[3.2.1]oc-
t-2-yl]carbonyl}amino)-3,4-dihydroisoquinoline-2(1H)-carboxylate
[0416] To a solution of the product of Step 2 (64.6 mg, 0.155 mmol)
in pyridine (1.5 mL) was added sulfur trioxide pyridine complex
(129.5 mg, 0.814 mmol). The mixture was stirred at room temperature
under nitrogen over the weekend. Dichloromethane was then added and
the mixture was filtered. The collected solid was washed thoroughly
with dichloromethane and the combined filtrates were concentrated
under vacuum to afford the crude title compound. The residue was
used without purification in the next step.
Step 4:
(2S,5R)-7-oxo-6-(sulfooxy)-N-(1,2,3,4-tetrahydroisoquinolin-6-yl)--
1,6-diazabicyclo[3.2.1]octane-2-carboxamide
[0417] To a solution of the product of Step 3 (77 mg, 0.155 mmol,
theoretical yield of Step 3) in anhydrous dichloromethane (3 mL) at
0.degree. C. under nitrogen was added trifluoroacetic acid (1 mL,
13 mmol) dropwise. The reaction mixture was stirred for one hour
then concentrated under vacuum. Ether was added to the residue and
the resulting white precipitate was collected by centrifugation
(ether trituration repeated two more times). The resulting solid
was purified by HPLC on a Phenomenex Synergy Polar-RP 80A column
eluted with methanol/water and lyophilized to afford the title
compound as a white solid. LC-MS (negative ionization mode) m/e 395
(M-H).
Example 17
(2S,5R)-7-Oxo-N-(5-piperidin-4-ylpyridin-2-yl)-6-(sulfooxy)-1,6-diazabicyc-
lo[3.2.1]octane-2-carboxamide
##STR00071## ##STR00072##
[0418] Step 1:
(2S,5R)-6-(benzyloxy)-N-(5-bromopyridin-2-yl)-7-oxo-1,6-diazabicyclo[3.2.-
1]octane-2-carboxamide
[0419] To a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (0.400 g, 1.448 mmol) in dry dichloromethane (17.66 mL) was
added triethylamine (0.504 ml, 3.62 mmol),
2-chloro-1-methylpyridinium iodide (0.433 g, 1.694 mmol), and
2-amino-5-bromopyridine (0.311 g, 1.795 mmol) sequentially at room
temperature under nitrogen. The reaction mixture was heated to
50.degree. C. for 1 hour then purified on the mass directed HPLC
(30.times.100 mm Waters Sunfire column; 5 micron; 50 ml/min;
acetonitrile/water with 0.1% TFA over 15 min). Fractions containing
the title compound were concentrated under vacuum then lyophilized
overnight to afford the title compound as a yellow solid.
Step 2:
6-({[(2S,5R)-6-(benzyloxy)-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]c-
arbonyl}amino)-3',6'-dihydro-3,4'-bipyridine-1'(2'H)-carboxylate
[0420]
tert-Butyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,6-dihy-
dropyridine-1(2H)-carboxylate (215 mg, 0.696 mmol) was added to the
product of Step 1 (150 mg, 0.348 mmol) in a reaction vial then
bis(triphenylphosphine)palladium(II) dichloride (24 mg, 0.035 mmol)
was added followed by 1M aqueous sodium carbonate (0.869 mL, 0.869
mmol) and acetonitrile (0.899 mL). The reaction mixture was
degassed then placed briefly in a preheated 70.degree. C. oil bath
then cooled to room temperature and filtered. The filtrate was
concentrated under vacuum and the residue was purified by column
chromatography on silica gel eluted with ethyl acetate/hexane
(0-50% over 1500 mL then 50-100% over 750 mL) to afford the title
compound as a yellow oil.
Step 3:
4-[6-({[(2S,5R)-6-hydroxy-7-oxo-1,6-diazabicyclo[3.2.1]oct-2-yl]ca-
rbonyl}amino)pyridin-3-yl]piperidine-1-carboxylate
[0421] To a mixture of the product of Step 2 (50 mg, 0.094 mmol) in
ethyl acetate (6 ml) was added 10% palladium on carbon (9.97 mg).
The reaction mixture was stirred under an atmosphere of hydrogen
(balloon) overnight then filtered. The filtrate was concentrated
under vacuum to afford the title compound as a colorless oil which
was used without purification in the next step.
Step 4:
4-[6-({[(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2.1]oct-2-yl-
]carbonyl}amino)pyridin-3-yl]piperidine-1-carboxylate
[0422] To a solution of the product of Step 3 (30 mg, 0.067 mmol)
in dry pyridine (1.5 ml) was added pyridine sulfur trioxide (53.6
mg, 0.337 mmol) at room temperature, in the dark, under nitrogen.
The reaction mixture was stirred over the weekend then filtered
(collected solid was washed well with dichloromethane). The
filtrate was concentrated under vacuum to afford the title compound
as a colorless oil which was used without purification in the next
step.
Step 5:
(2S,5R)-7-oxo-N-(5-piperidin-4-ylpyridin-2-yl)-6-(sulfooxy)-1,6-di-
azabicyclo[3.2.1]octane-2-carboxamide
[0423] To a solution of the product of Step 4 (35 mg, 0.067 mmol;
theoretical yield of step 4) in dry dichloromethane (3 mL) was
added trifluoroacetic acid (0.00513 mL, 0.067 mmol) at 0.degree. C.
under nitrogen. The reaction mixture was stirred for 30 minutes
then concentrated under vacuum. The residue was triturated with
ether to remove excess trifluoroacetic acid and organic-soluble
impurities. The resulting solid was dried, dissolved in water, and
purified by prep HPLC on a Phenomenex Synergy Polar-RP 80A column
eluted with methanol/water and lyophilized to afford the title
compound. LC-MS (negative ionization mode) m/e 424 (M-H).
Example 18
[0424]
Piperidin-4-ylmethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.2-
.1]octane-2-carboxylate
##STR00073##
Step 1:
[1-(tert-butoxycarbonyl)piperidin-4-yl]methyl(2S,5R)-6-(benzyloxy-
)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxylate
[0425] N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride
(109 mg, 0.57 mmol) and 4-dimethylaminopyridine (69.6 mg, 0.57
mmol) were added sequentially to a solution of
(2S,5R)-6-(phenylmethoxy)-7-oxo-1,6-diazabicyclo[3.2.1]octane-2-carboxyli-
c acid (105 mg, 0.38 mmol) in dry dichloromethane at room
temperature. tert-Butyl 4-(hydroxymethyl)piperidine-1-carboxylate
(123 mg, 0.57 mmol) was then added and the reaction mixture was
stirred at room temperature overnight, and then concentrated under
vacuum. The residue was purified by preparative HPLC to afford the
title compound.
Step 2:
[1-(tert-butoxycarbonyl)piperidin-4-yl]methyl(2S,5R)-6-hydroxy-7-o-
xo-1,6-diazabicyclo[3.2.1]octane-2-carboxylate
[0426] To a mixture of the product of Step 1 (100 mg, 0.211 mmol)
in methanol was added 10% palladium on carbon (6.74 mg). The
reaction mixture was stirred under an atmosphere of hydrogen
(balloon) overnight then filtered. The filtrate was concentrated
under vacuum to afford the title compound which was used without
purification in the next step.
Step 3:
[1-(tert-butoxycarbonyl)piperidin-4-yl]methyl(2S,5R)-7-oxo-6-(sulf-
ooxy)-1,6-diazabicyclo[3.2.1]octane-2-carboxylate
[0427] To a solution of the product of Step 2 (50 mg, 0.13 mmol) in
dry pyridine (1 mL) was added pyridine sulfur trioxide (104 mg,
0.652 mmol) at room temperature under nitrogen. The reaction
mixture was stirred overnight then filtered (collected solid was
washed well with dichloromethane). The filtrate was concentrated
under vacuum to afford the title compound which was used without
purification in the next step.
Step 4:
piperidin-4-ylmethyl(2S,5R)-7-oxo-6-(sulfooxy)-1,6-diazabicyclo[3.-
2.1]octane-2-carboxylate
[0428] TFA was added to the product of Step 3 at 0.degree. C. under
nitrogen. The reaction mixture was stirred for 1 hour then
concentrated under vacuum. The residue was triturated with ether to
remove excess trifluoroacetic acid and organic-soluble impurities.
The resulting solid was dried, dissolved in water, and purified by
preparative HPLC on a Phenomenex Synergy Polar-RP 80A column eluted
with methanol/water and lyophilized to afford the title compound.
LC-MS (negative ionization mode) m/e 362 (M-H).
Examples 19-56
[0429] The procedure set forth in Example 1A was used to prepare
the following compounds, wherein the indicated amine starting
material was substituted for 4-amino-1-BOC-piperidine in Step
1.
TABLE-US-00002 Example Amine Product 19A & 19B ##STR00074##
##STR00075## 20 ##STR00076## ##STR00077## 21 ##STR00078##
##STR00079## 22 ##STR00080## ##STR00081## 23 ##STR00082##
##STR00083## 24 ##STR00084## ##STR00085## 25 ##STR00086##
##STR00087## 26 ##STR00088## ##STR00089## 27 ##STR00090##
##STR00091## 28 ##STR00092## ##STR00093## 29 ##STR00094##
##STR00095## 30 ##STR00096## ##STR00097## 31 ##STR00098##
##STR00099## 32 ##STR00100## ##STR00101## 33 ##STR00102##
##STR00103## 34 ##STR00104## ##STR00105## 35 ##STR00106##
##STR00107## 36 ##STR00108## ##STR00109## 37 ##STR00110##
##STR00111## 38 ##STR00112## ##STR00113## 39 ##STR00114##
##STR00115## 40 ##STR00116## ##STR00117## 41 ##STR00118##
##STR00119## 42 ##STR00120## ##STR00121## 43 ##STR00122##
##STR00123## 44 ##STR00124## ##STR00125## 45 ##STR00126##
##STR00127## 46 ##STR00128## ##STR00129## 47 ##STR00130##
##STR00131## 48 ##STR00132## ##STR00133## 49 ##STR00134##
##STR00135## 50 ##STR00136## ##STR00137## 51 ##STR00138##
##STR00139## 52 ##STR00140## ##STR00141## 53 ##STR00142##
##STR00143## 54 ##STR00144## ##STR00145## 55 ##STR00146##
##STR00147## 56 ##STR00148## ##STR00149##
Examples 57-90
[0430] The procedure set forth in Example 6 was used to prepare the
compounds of Examples 57-77 and 80-90, wherein the indicated amine
starting material was substituted for 4-aminopyridine in Step 1.
The procedure set forth in Example 17 was used to prepare the
compounds of Examples 78 and 79, wherein the indicated pyridine
starting material was substituted for 2-amino-5-bromopyridine in
Step 1.
TABLE-US-00003 Example Amine Product 57 ##STR00150## ##STR00151##
58 ##STR00152## ##STR00153## 59 ##STR00154## ##STR00155## 60
##STR00156## ##STR00157## 61 ##STR00158## ##STR00159## 62
##STR00160## ##STR00161## 63 ##STR00162## ##STR00163## 64
##STR00164## ##STR00165## 65 ##STR00166## ##STR00167## 66
##STR00168## ##STR00169## 67 ##STR00170## ##STR00171## 68
##STR00172## ##STR00173## 69 ##STR00174## ##STR00175## 70
##STR00176## ##STR00177## 71 ##STR00178## ##STR00179## 72
##STR00180## ##STR00181## 73 ##STR00182## ##STR00183## 74
##STR00184## ##STR00185## 75 ##STR00186## ##STR00187## 76
##STR00188## ##STR00189## 77 ##STR00190## ##STR00191## 78
##STR00192## ##STR00193## 79 ##STR00194## ##STR00195## 80
##STR00196## ##STR00197## 81 ##STR00198## ##STR00199## 82
##STR00200## ##STR00201## 83 ##STR00202## ##STR00203## 84
##STR00204## ##STR00205## 85 ##STR00206## ##STR00207## 86
##STR00208## ##STR00209## 87 ##STR00210## ##STR00211## 88
##STR00212## ##STR00213## 89 ##STR00214## ##STR00215## 90
##STR00216## ##STR00217##
Examples 91-117
[0431] The procedure set forth in Example 18 was used to prepare
the following compounds, wherein the indicated alcohol starting
material was substituted for tert-butyl
4-(hydroxymethyl)piperidine-1-carboxylate in Step 1.
TABLE-US-00004 Example Amine Product 91 ##STR00218## ##STR00219##
92 ##STR00220## ##STR00221## 93 ##STR00222## ##STR00223## 94
##STR00224## ##STR00225## 95 ##STR00226## ##STR00227## 96
##STR00228## ##STR00229## 97 ##STR00230## ##STR00231## 98
##STR00232## ##STR00233## 99 ##STR00234## ##STR00235## 100
##STR00236## ##STR00237## 101 ##STR00238## ##STR00239## 102
##STR00240## ##STR00241## 103 ##STR00242## ##STR00243## 104
##STR00244## ##STR00245## 105 ##STR00246## ##STR00247## 106
##STR00248## ##STR00249## 107 ##STR00250## ##STR00251## 108
##STR00252## ##STR00253## 109 ##STR00254## ##STR00255## 110
##STR00256## ##STR00257## 111 ##STR00258## ##STR00259## 112
##STR00260## ##STR00261## 113 ##STR00262## ##STR00263## 114
##STR00264## ##STR00265## 115 ##STR00266## ##STR00267## 116
##STR00268## ##STR00269## 117 ##STR00270## ##STR00271##
Example 118
Enzyme Activity Determination of IC.sub.50
[0432] The Class C enzyme activities were measured in the presence
of the test inhibitor in spectrophotometric assay against the
commercially available substrate, nitrocefin. The enzyme AmpC (P.
aeruginosa.), and the substrate, were dissolved in 100 mM
KH.sub.2PO.sub.4 buffer (pH 7). The buffer also contains 0.005%
BSA. The test inhibitor was dissolved in DMSO and diluted 1:20 in
the assay, resulting in a final concentration range of 50 .mu.M to
0.0002 .mu.M. In a 96-well microplate, the test inhibitor was
incubated with the beta-lactamase enzyme for 40 minutes at ambient
temperature, the substrate solution was added, and the incubation
continued for another 40 minutes. The spectrophotometric reaction
was quenched by the addition of 2.5N acetic acid and the absorbance
at 492 nm was measured. The IC.sub.50 value was determined from
semi logarithmic plots of enzyme inhibition versus inhibitor
concentration, with a curve generated using a 4-parameter fit.
[0433] The Class A enzyme activities were measured using the same
test protocol set forth above for Class C enzymes except that the
enzyme KPC-2 (K. pneumoniae) replaced AmpC.
[0434] Representative compounds of the present invention exhibit
inhibition of Class C and Class A .beta.-lactamases in this assay.
For example, the compounds of Examples 1, 2, 4 and 6-9 were tested
in this assay and were found to have the IC.sub.50 values shown in
Table 2. Table 3 contains assay data for other exemplified
compounds.
Synergy Assay Protocol:
[0435] The assay determines the concentration of a .beta.-lactamase
inhibitor required to reduce the MIC of a .beta.-lactam antibiotic
by one-half, one-quarter, one-eighth, one-sixteenth and
one-thirty-second against strains of bacteria normally resistant to
the antibiotic in question. This is accomplished by titrating the
BLI in a serial dilution across a microtiter plate while at the
same time titrating the antibiotic in a serial dilution down the
microtiter plate and then inoculating the plate with the bacterial
strain in question and allowing the bacteria to grow up overnight.
Each well in this microplate checkerboard contains a different
combination of concentrations of the inhibitor and the antibiotic
allowing a full determination of any synergy between the two.
Bacterial Strain/Antibiotic Combinations:
[0436] CL 5701 (Pseudomonas aeruginosa; Pa AmpC)/Imipenem
[0437] MB 2646 (Enterobacter cloacae; P99)/Ceftazidime
[0438] CL 5513 (Klebsiella pneumoniae; SHV-5)/Ceftazidime
[0439] CL 6188 (Acinetobacter baumanii; Oxa40)/Imipenem
[0440] CL 6569 (Klebsiella pneumoniae; KPC-2)/Imipenem
[0441] CL 5761 (Klebsiella pneumoniae; KPC-3)/Imipenem
[0442] CLB 21648 (Acinetobacter baumanii; Ab AmpC)/Imipenem
General Checkerboard Method:
[0443] 1. All wells in rows B-H of MIC 2000 microtiter plates are
filled with 100 .mu.L of MHBII+1% DMSO. [0444] 2. All wells in row
A of MIC 2000 microtiter plates are filled with 100 .mu.L of
2.times.MHBII+2% DMSO. [0445] 3. 100 .mu.L of 4.times. the final
antibiotic concentration wanted is added to well A1 of the MIC 2000
plates. [0446] 4. 100 .mu.L of 2.times. the final antibiotic
concentration wanted is added to wells A2-A12 of the MIC 2000
plates. [0447] 5. 100 .mu.L is serially diluted from row A to row G
of each MIC 2000 plate. [0448] 6. 100 .mu.L is removed from each
well in row G of each MIC 2000 plate. [0449] 7. 100 .mu.L of
2.times. the final inhibitor concentration wanted (in MHBII+1%
DMSO) is added to all wells in column 1 of the microtiter plates.
[0450] 8. 100 .mu.L is serially diluted from column 1 to column 11
of each MIC 2000 plate. [0451] 9. 100 .mu.L is removed from each
well in column 11 of each MIC 2000 plate. [0452] 10. Plates are
then inoculated with an overnight growth (in TSB) of the strain to
be tested using an MIC 2000 inoculator. [0453] 11. Plates are left
at 37.degree. C. for about 20 hours and scored for growth by
eye.
Media (all are Sterilized by Autoclaving Prior to any Addition of
DMSO):
TABLE-US-00005 [0454] MHBII + 1% DMSO MHBII cation adjusted (BBL
.TM.) 4.4 g DMSO 2.0 mL Distilled water 198.0 mL 2X MHBII + 2% DMSO
MHBII cation adjusted (BBL .TM.) 8.8 g DMSO 4.0 mL Distilled water
196.0 mL 1.02X MHBII MHBII cation adjusted (BBL .TM.) 4.4 g
Distilled water 198.0 mL 1.1 X MHBII + 1% DMSO MHBII cation
adjusted (BBL .TM.) 4.4 g DMSO 2.0 mL Distilled water 178.0 mL
TSB
[0455] Trypticase soy roth (BBL.TM.) was prepared as directed on
the bottle.
[0456] Synergy may be expressed as a ratio of the minimum
inhibitory concentration (MIC) of an antibiotic tested in the
absence of a .beta.-lactamase inhibitor to the MIC of the same
antibiotic tested in the presence of the .beta.-lactamase
inhibitor. A ratio of one (1) indicates that the .beta.-lactamase
inhibitor has no effect on antibiotic potency. A ratio greater than
one (1) indicates that the .beta.-lactamase inhibitor produces a
synergistic effect when co-administered with the antibiotic agent.
The preferred .beta.-lactamase inhibitors of the present invention
exhibit a synergy ratio of at least about 2, more preferred
compounds exhibit a ratio of at least about 4, still more
preferably at least about 8, and most preferred at least about 16.
Alternatively, the synergy effect may be expressed as a factor,
again, utilizing a concentration of the BLI to lower the MIC of the
antibiotic. Thus, if the MIC of the antibiotic is 20 .mu.g/mL and a
1.5 .mu.M concentration of BLI lowers the MIC to 5 .mu.g/mL, the
synergy effect is four fold or "4.times. synergy" at 1.5 .mu.M of
BLI.
[0457] Representative compounds of the present invention display a
synergy effect. For example, the compounds of Examples 1, 2, 4 and
6-9 were determined to have 2.times. synergy concentrations in a
range of from about 100 .mu.M or less. The synergy concentrations
for Examples 1, 2, 4 and 6-9 against P. aeruginosa strain CL5701
and Klebsiella pneumoniae strain CL6569 are shown in Table 2.
TABLE-US-00006 TABLE 2 Biological Data R (* indicates P.a. 2X/4X/8X
K.p. 16X/32X/64X point of AmpC Synergy KPC-2 Synergy CL6569 Example
attachment) IC.sub.50 (nM) CL5701 (.mu.M).sup.1 IC.sub.50 (nM)
(.mu.M).sup.2 1 ##STR00272## 465 0.2/3.12/6.25 208 6.25/12.5/12.5 2
##STR00273## 69 3.12/6.25/6.25 245 6.25/12.5/25 4 ##STR00274##
29,000 100/100/>100 4,400 50/100/100 6 ##STR00275## 6
12.5/25/100 54 50/50/>100 7 ##STR00276## 1.1 50/100/>100 8
25/50/>100 8 ##STR00277## 1.6 12.5/100/>100 1.6 25/25/50 9
##STR00278## 20 0.78/3.12/6.25 72 6.25/6.25/12.5 Sulbactam --
17,000 54/>150/>300 33,000 >500/>500/>500
.sup.1These are the concentrations for 2X, 4X and 8X with imipenem
against P. aeruginosa strain CL5701. For example, a 6.25 .mu.M
concentration of the compound of Example 1 reduces the MIC of
imipenem versus P. aeruginosa strain CL5701 by a factor of 8 (8X
synergy). .sup.2These are the concentrations for 16X, 32X and 64X
with imipenem against K. pneumoniae strain CL6569. For example, a
12.5 .mu.M concentration of the compound of Example 1 reduces the
MIC of imipenem versus K. pneumoniae strain CL6569 versus by a
factor of 64 (64X synergy).
[0458] It appears that the data in Table 3 were generated in the
same manner using the same enzymes as in Table 2 (Table 2 is
unchanged from the provisional filing). I plan to DELETE the
entries in Table 3 for Exs. 2, 6, 7 and 8 because this data
duplicates the data in Table 2. HOWEVER, the entry in Table 2 for
Ex. 7 is "1.1" NOT "11" as shown in Table 3. Also, the entries in
Table 2 for Ex. 8 are both "1.6", not "16" as shown in Table 3.
Please clarify these differences.
[0459] Is the data shown for Ex. 1A data generated in a separate
run, or is it supposed to be the same as for Ex. 1? Note "208" in
Table 2 vs, "210" in Table 3--why different? I propose to delete
the entry for Example 1A from Table 3 as it's essentially the same
as the entry in Table 2 for Example 1.
[0460] You don't want to include synergy data for these compounds?
It would be helpful to include it, at least for some of the
examples (could put in a separate table). Recommend we include it
for Ex. 14, since this is a likely backup candidate.
[0461] We have a comparison to sulbactam in Table 2. How does
MK-8712 compare to this? Is it worth including 8712 comparative
data? Any other structurally similar known compounds that you may
have run a comparison?
TABLE-US-00007 TABLE 3 Biological Data K.p. Example KPC-2 P.a. AmpC
No. M - H (m/e).sup.1 IC.sub.50 (nM) IC.sub.50 (nM) Plan to delete
the entries shown in blue 1A 347 210 465 2 361 245 69 6 341 54 6 7
371 8 11 8 384 16 16 14 333 355 110 15 365 130 49 16 395 19 14 17
424 28 6 18 362 10 180 19a 347 480 64 19b 347 240 480 20 319 17
1,000 21 333 4,300 29,100 22 361 225 520 23 361 240 500 24 365 90
110 25 365 270 20 26 377 290 120 27 396 150 9.5 28 348 660 740 29
349 250 2,500 30 349 520 2,250 31 351 190 150 32 351 710 19 33 430
21 620 34 430 280 45 35 379 150 13 36 379 130 18 37 351 120 40 38
347 120 250 39 361 190 530 40 361 77 30 41 391 320 700 42 379 110
100 43 379 100 15 44 375 45 11 45 375 150 180 46 376 96 42 47 345
10,000 720 48 361 2,500 290 49 361 780 7,500 50 347 8 820 51 333
520 2,200 52 373 1,000 590 53 359 33 1,000 54 333 3,200 840 55 333
1,500 1,400 56 361 1,600 270 57 369 72 20 58 369 104 34 59 369 109
292 60 383 56 18 61 383 78 22 62 397 54 18 63 423 85 22 64 395 19
14 65 381 60 16 66 381 41 56 67 384 10 11 68 383 30 7 69 419 22 7
70 383 26 3 71 341 42 37 72 341 36 1.2 73 478 130 515 74 356 11 8
75 384 410 3 76 370 1,300 2 77 370 42 3 78 424 470 0.8 79 424 24
270 80 425 15 2 81 427 17 5 82 410 10 4 83 342 58 6 84 342 55 0.8
85 426 20 260 86 344 135 240 87 346 840 200 88 347 36 1.8 89 402 39
10 90 382 NA NA 91 362 6 83 92 362 6,400 33,000 93 348 9 170 94 334
40 3,000 95 334 21 210 96 320 18 600 97 366 26 49 98 366 9 46 99
366 8 40 100 366 4 33 101 366 15 81 102 366 4 76 103 352 3 39 104
352 24 38 105 352 13 200 106 352 10 200 107 336 11 39 108 336 11 62
109 335 62 700 110 308 30 610 111 376 20 1,200 112 376 13 165 113
376 8 36 114 378 11 340 115 370 2 24 116 528 70 440 117 777 12 61
.sup.1Values obtained via LC-MS (negative ion mode).
[0462] While the foregoing specification teaches the principles of
the present invention, with examples provided for the purpose of
illustration, the practice of the invention encompasses all of the
usual variations, adaptations and/or modifications that come within
the scope of the following claims. All publications, patents and
patent applications cited herein are incorporated by reference in
their entireties into the disclosure, wherein in the case of any
inconsistencies, the present disclosure will prevail.
* * * * *