U.S. patent application number 13/885156 was filed with the patent office on 2013-09-19 for method for supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves.
This patent application is currently assigned to SHANGHAI TOBACCO GROUP CO., LTD.. The applicant listed for this patent is Baizhan Liu, Wenliang Sun, Haobo Zhang. Invention is credited to Baizhan Liu, Wenliang Sun, Haobo Zhang.
Application Number | 20130239981 13/885156 |
Document ID | / |
Family ID | 43782330 |
Filed Date | 2013-09-19 |
United States Patent
Application |
20130239981 |
Kind Code |
A1 |
Sun; Wenliang ; et
al. |
September 19, 2013 |
METHOD FOR SUPERCRITICAL EXTRACTION OF CHARACTERISTIC FRAGRANT
SUBSTANCES IN DIFFERENT FRAGRANT TOBACCO LEAVES
Abstract
A supercritical extraction of characteristic fragrant substances
in different fragrant tobacco leaves, which involves the following
steps: 1) cutting the tobacco leaves into shreds and placing the
shreds into an extraction vessel with supercritical CO.sub.2 fluid;
2) setting the extraction pressure and extraction temperature of
supercritical CO.sub.2 fluid about 100 to 350 bar and 35 to
70.degree. C., extracting statically the tobacco shreds for 10 to
35 minutes in the condition, then setting the dynamic extracting
speed of the supercritical CO.sub.2 fluid about 1.0 to 3.5 L/min,
and extracting dynamically the tobacco shreds for 5 to 15 minutes;
3) collecting the obtained supercritical CO.sub.2 fluid extract,
and obtaining the key fragrant substances, which act as the
determinants for tobacco fragrance, after completely volatilizing
CO.sub.2.
Inventors: |
Sun; Wenliang; (Shanghai,
CN) ; Zhang; Haobo; (Shanghai, CN) ; Liu;
Baizhan; (Shanghai, CN) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Sun; Wenliang
Zhang; Haobo
Liu; Baizhan |
Shanghai
Shanghai
Shanghai |
|
CN
CN
CN |
|
|
Assignee: |
SHANGHAI TOBACCO GROUP CO.,
LTD.
Shanghai
CN
|
Family ID: |
43782330 |
Appl. No.: |
13/885156 |
Filed: |
December 16, 2010 |
PCT Filed: |
December 16, 2010 |
PCT NO: |
PCT/CN2010/079879 |
371 Date: |
May 13, 2013 |
Current U.S.
Class: |
131/297 |
Current CPC
Class: |
A24B 15/241 20130101;
A24B 15/24 20130101 |
Class at
Publication: |
131/297 |
International
Class: |
A24B 15/24 20060101
A24B015/24 |
Foreign Application Data
Date |
Code |
Application Number |
Nov 29, 2010 |
CN |
201010563298.9 |
Claims
1. A method for supercritical extraction of characteristic fragrant
substances in different fragrant tobacco leaves, comprising: 1)
pretreatment of tobacco leaves: shredding the tobacco leaves and
placing the tobacco shreds into an extraction vessel with
supercritical CO.sub.2 fluid; 2) setting the extraction pressure
and the extraction temperature of the supercritical CO.sub.2 fluid
to 100 to 350 bar and 35.degree. C. to 70.degree. C., performing
static extraction on the tobacco shreds for 10 to 35 min under the
condition, then setting the dynamic extraction flow rate of the
supercritical CO.sub.2 fluid to 1.0 to 3.5 L/min, and performing
dynamic extraction on the tobacco shreds for 5 to 15 min; and 3)
collecting the obtained supercritical CO.sub.2 fluid extract, and
obtaining key fragrant substances that act as determinants after
complete evaporation of CO.sub.2.
2. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to claim 1, wherein the used CO.sub.2 is food grade CO.sub.2.
3. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to claim 1, wherein in Step 1), the width of the tobacco shreds is
0.8 mm to 1.2 mm.
4. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to claim 1, wherein in Step 2), the extraction pressure and the
extraction temperature of the supercritical CO.sub.2 fluid are set
to 250 to 320 bar and 45 to 55.degree. C.
5. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to claim 1, wherein in Step 2), static extraction is performed on
the tobacco shreds for 20 to 30 min.
6. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to claim 1, wherein in Step 2), the dynamic extraction flow rate of
the supercritical CO.sub.2 fluid is set to 2.0 to 3.5 L/min.
7. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to claim 1, wherein in Step 2), dynamic extraction is performed on
the tobacco shreds for 10 to 15 min.
8. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 1, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
9. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 2, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
10. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 3, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
11. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 4, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
12. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 5, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
13. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 6, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
14. The method for supercritical extraction of characteristic
fragrant substances in different fragrant tobacco leaves according
to any one of claim 7, wherein the tobacco leaves comprise three
types of typical fragrance tobacco leaves and intermediary
fragrance tobacco leaves, the three typical fragrances are strong
fragrance, mild fragrance, and intermediate fragrance, the
intermediary fragrances comprise strong-to-intermediate fragrance,
intermediate-to-strong fragrance, mild-to-intermediate fragrance,
intermediate-to-mild fragrance, mild-over-strong fragrance, and
strong-over-mild fragrance.
Description
BACKGROUND OF THE INVENTION
[0001] 1. Technical Field of the Invention
[0002] The present invention relates to a method for extraction of
characteristic fragrant substances in tobacco leaves, and in
particular, to a method for supercritical CO.sub.2 fluid extraction
of characteristic fragrant substances in different fragrant tobacco
leaves, and belongs to the tobacco field.
[0003] 2. Description of the Related Art
[0004] The fragrances of flue-cured tobacco leaves are mainly
classified into strong fragrance, mild fragrance and intermediate
fragrance, and further include intermediary fragrances such as
strong-to-intermediate fragrance, intermediate-to-strong fragrance,
mild-to-intermediate fragrance, intermediate-to-mild fragrance,
mild-over-strong fragrance, and strong-over-mild fragrance. After
long-term development, characteristic types of tobacco leaves with
certain geographical features have been formed in production of
tobacco leaves in China; for example, mild fragrance flue-cured
tobacco in Jiangchuan and Yuxi, Yu'nan, mild fragrance flue-cured
tobacco in Yongding, Fujian, intermediate fragrance flue-cured
tobacco in Guiding, Guizhou, and strong fragrance flue-cured
tobacco in Xiangcheng and Jiaxian, He'nan. In summary, the
fragrance style of flue-cured tobacco leaves in China has gradually
changed from intermediate fragrance and strong fragrance to mild
fragrance as a whole from northeast to southwest. For example, over
90% of tobacco leaves in Kaiyuan, Liaoning belong to intermediate
fragrance, 51% of tobacco leaves in He'nan belong to
intermediate-to-mild fragrance, 50% of tobacco leaves in Meitan,
Guizhou belong to intermediate fragrance, and the other 50% belongs
to intermediate-to-mild fragrance, 68% of tobacco leaves in
Ningnan, Sichuan belong to intermediate-to-mild fragrance, over 70%
of tobacco leaves in Yongding, Fujian belong to
intermediate-to-mild and mild fragrances, 51% of tobacco leaves in
Wenshan, Yunan belong to intermediate-to-mild fragrance, and so on.
Although the classification of the three types of fragrances of
tobacco leaves has been used, the evaluation mainly depends on
sensory evaluation through smoking, and qualitative and
quantitative indicators are absent.
[0005] The basis of the style of cigarette lies in the raw tobacco
leaves, while the key of the raw tobacco leaves lies in the
fragrance style of the tobacco leaves. Studies show that chemical
ingredients are the material basis for the formation of fragrance
features of tobacco leaves. In-depth analysis of the chemical
composition of different fragrant tobacco leaves and research on
the correlation between important chemical ingredients and the
fragrance of tobacco leaves have important practical significance
for understanding and improving the intrinsic quality and
availability of tobacco leaves, enhancing the regulation and
control capability on cigarette formulation, and guiding
procurement of tobacco leaves.
[0006] Supercritical fluid extraction (SFE) is an extraction and
separation technology that has developed rapidly at home and abroad
in recent years. The supercritical fluid is a high-density fluid
that is subject to a temperature and pressure higher than the
critical temperature and the critical pressure, and has a high
dissolving capability, high extraction rate, and good transmission
performance. Small changes in temperature and pressure may result
in a large change in solubility of the solute. By changing physical
parameters (temperature and pressure), the dissolving capability of
the fluid can be easily changed, thereby achieving the purpose of
selective extraction and separation.
[0007] Commonly used supercritical fluid includes methanol,
ethanol, ethane, propane, ethylene, and CO.sub.2. CO.sub.2 has the
following advantages: being capable of working at a low
temperature, causing no changes of thermally unstable substances,
having a low critical pressure that can be easily achieved in
actual operation, being non-toxic, colorless, and odorless, having
no pollution to products, being chemically stable, inexpensive and
easily available. Consequently, in processing of natural products,
CO.sub.2 is usually used as a working medium.
[0008] Extraction of nicotine in tobacco leaves with a
supercritical fluid has been reported, but a method for extraction
of characteristic fragrant substances in different fragrant tobacco
leaves through SFE has not been reported.
SUMMARY OF THE INVENTION
[0009] The present invention is directed to a method for extraction
of characteristic fragrant substances in tobacco leaves, which is
applicable to different fragrant tobacco leaves, so as to establish
the foundation for providing a unified method for evaluating
fragrance styles of tobacco leaves.
[0010] The technical solution adopted by the present invention is a
method for supercritical extraction of characteristic fragrant
substances in tobacco leaves of different fragrances, which
includes the following steps:
[0011] 1) pretreatment of tobacco leaves: shredding the tobacco
leaves and placing the tobacco shreds into an extraction vessel
with supercritical CO.sub.2 fluid;
[0012] 2) setting the extraction pressure and the extraction
temperature of the supercritical CO.sub.2 fluid to 100 to 350 bar
and 35.degree. C. to 70.degree. C., performing static extraction on
the tobacco shreds for 10 to 35 min under the condition, then
setting the dynamic extraction flow rate of the supercritical
CO.sub.2 fluid to 1.0 to 3.5 L/min, and performing dynamic
extraction on the tobacco shreds for 5 to 15 min; and
[0013] 3) collecting the obtained supercritical CO.sub.2 fluid
extract, and obtaining key fragrant substances that act as
determinants after complete evaporation of CO.sub.2.
[0014] The used CO.sub.2 is food grade CO.sub.2.
[0015] Preferably, the width of the tobacco shreds is 0.8 mm to 1.2
mm. The tobacco leaves are cut into shreds of 0.8 mm to 1.2 mm to
fully infiltrate the tobacco leaves.
[0016] Preferably, in Step 2), the extraction pressure and the
extraction temperature of the supercritical CO.sub.2 fluid are set
to 250 to 320 bar and 45.degree. C. to 55.degree. C.
[0017] Preferably, in Step 2), static extraction is performed on
the tobacco shreds for 20 to 30 min.
[0018] Preferably, in Step 2) the dynamic extraction flow rate of
the supercritical CO.sub.2 fluid is set to 2.0 to 3.5 L/min.
[0019] Preferably, in Step 2), dynamic extraction is performed on
the tobacco shreds for 10 to 15 min.
[0020] Said tobacco leaves include three types of typical fragrance
tobacco leaves and intermediary fragrance tobacco leaves. The three
typical fragrances are strong fragrance, mild fragrance and
intermediate fragrance, and the intermediary fragrances include
strong-to-intermediate fragrance, intermediate-to-strong fragrance,
mild-to-intermediate fragrance, intermediate-to-mild fragrance,
mild-over-strong fragrance, and strong-over-mild fragrance.
[0021] The key fragrant substances that act as determinants
obtained through static extraction and dynamic extraction in
combination are dissolved in anhydrous ethanol, and analyzed by gas
chromatography-mass spectrometry. The results show that the types
of fragrant ingredients contained in the different fragrant tobacco
leaves are substantially the same, but the contents of the
ingredients are different; the fragrant ingredients in the tobacco
can be substantially extracted through one-time extraction. After
the extract is dissolved in anhydrous ethanol, the solution is
sprayed on the tobacco shreds after the supercritical CO.sub.2
fluid extraction, and after ethanol evaporation, the tobacco shreds
are rolled into cigarettes, which are then subjected to evaluation
smoking by experts. The tobacco shreds are compared with tobacco
shreds that are not subjected to supercritical CO.sub.2 fluid
extraction, and the result shows that the fragrances of the two
types of tobacco shreds are fully the same. This comparison
indicates that the supercritical CO.sub.2 extraction method
provided by the present invention is applicable in extraction of
characteristic fragrant substances in different tobacco leaves.
[0022] In view that the fragrance style of tobacco leaves is
currently determined primarily through evaluation smoking by
experts, and unified objective standards are absent, the present
invention provides a method for extraction of characteristic
fragrant substances, which is applicable to different fragrant
tobacco leaves. By analyzing the correlation between the fragrant
ingredients in the extract obtained through the method and the
fragrance of tobacco leaves, the internal relationship between the
type and the content of the fragrant ingredient and the fragrance
of tobacco leaves could be found, so that the analysis of fragrant
ingredients extracted from the tobacco leaves can be used as an
objective standard for evaluation of the fragrance of tobacco
leaves. Therefore, the extraction method of the present invention
is an efficient means for objectively estimating the fragrance of
tobacco leaves, and establishes the foundation for providing a
unified method for evaluation of fragrance styles of tobacco
leaves.
[0023] In the present invention, food grade supercritical CO.sub.2
fluid is used as the working medium, and the characteristic
fragrant substances in different tobacco leaves are extracted
through static extraction and dynamic extraction in combination. In
the extraction method of the present invention, no entrainer is
used, and the method has the advantages of being safe and
non-toxic, is a simple and reproducible process, and has high
reliability.
BRIEF DESCRIPTION OF THE DRAWINGS
[0024] The present disclosure will become more fully understood
from the detailed description given herein below for illustration
only, and thus does not limit the present disclosure, wherein:
[0025] FIG. 1 is a process flow chart of an extraction method
according to the present invention;
[0026] FIG. 2 is a gas chromatography/mass spectrometry analysis
chromatogram of SFE extract of Guizhou intermediate-fragrance
tobacco leaves;
[0027] FIG. 3 is a gas chromatography/mass spectrometry analysis
chromatogram of SFE extract of Yunnan mild-fragrance tobacco
leaves;
[0028] FIG. 4 is a gas chromatography/mass spectrometry analysis
chromatogram of SFE extract of He'nan strong-fragrance tobacco
leaves; and
[0029] FIG. 5 shows results of a repeatability test of an
extraction method.
DETAILED DESCRIPTION OF THE INVENTION
[0030] The present invention is further described below with
reference to the embodiments. It should be understood that, these
embodiment are merely used to illustrate the present invention, but
are not intended to limit the scope of the present invention.
Embodiment 1
[0031] Key fragrant ingredients in Guizhou intermediate-fragrance
tobacco leaves, Yunnan mild-fragrance tobacco leaves, and He'nan
strong-fragrance tobacco leaves were each extracted by adopting the
following method, including the following steps:
[0032] 1) cutting tobacco leaves into shreds of 0.8 mm to 1.2
mm;
[0033] 2) placing 5 g tobacco shreds into an extraction vessel with
supercritical CO.sub.2 fluid;
[0034] 3) setting the static extraction pressure and the extraction
temperature of the supercritical CO.sub.2 fluid to 290 bar and
50.degree. C.;
[0035] 4) performing static extraction on the tobacco shreds for 30
min under a static extraction condition;
[0036] 5) setting the CO.sub.2 dynamic extraction flow rate to 2.5
L/min;
[0037] 6) performing dynamic extraction on the tobacco shreds for
10 min under a dynamic extraction condition;
[0038] 7) collecting the supercritical CO.sub.2 fluid extract by
using a clean glass bottle, and after complete evaporation of
CO.sub.2, adding 3 mL anhydrous ethanol to dissolve the extract for
analysis of the ingredients of the extract.
[0039] The ethanol solutes, obtained through supercritical CO.sub.2
fluid extraction, of the three types of tobacco leaves were
analyzed by using gas chromatography-mass spectrometry. The results
are shown in FIGS. 2 to 4. The analysis results show that the SFE
extract in different fragrant tobacco leaves contains organic acids
such as 2-methyl butyric acid, 3-methyl valeric acid, n-nonanoic
acid, oleic acid, linoleic acid, linolenic acid, and stearic acid,
and further contains neutral fragrant ingredients such as solanone,
norsolanone, phenethyl alcohol, megastigmatrienone,
3-oxo-.alpha.-ionol and 3-OH-.beta.-damascone, 2-acetylpyrrole, and
fragrant precursors such as cembratrienediol, thunbergol, and
phytol. The types of fragrant ingredients contained in He'nan
strong-fragrance tobacco leaves, Guizhou intermediate-fragrance
tobacco leaves, and Yunnan mild-fragrance tobacco leaves are
substantially the same, but the contents of the fragrant
ingredients are different.
Embodiment 2
[0040] By adopting the extraction method in Embodiment 1, the three
types of tobacco shred samples were subjected to continuous
five-time supercritical CO.sub.2 fluid extraction, and the extract
of each type of tobacco leaves obtained by each extraction was
subjected to gas chromatography/mass spectrometry analysis. The
analysis results show that, with the increase of the number of
times of extraction, the content of the fragrant ingredients in the
extracts of the three types of tobacco leaves (tobacco shreds) are
significantly decreased, and the content of the fragrant
ingredients in the first-time extract is much greater than those of
the following extracts, indicating that the fragrant ingredients in
the tobacco leaves can be substantially extracted through one-time
extraction.
[0041] The three types of tobacco leaves (tobacco shreds) after the
supercritical CO.sub.2 fluid extraction were rolled into
cigarettes, subjected to smoke panel tests, and compared with
tobacco leaves (tobacco shreds) that are not subjected to SFE
extraction. The results of the smoke tests are listed in Table
1.
TABLE-US-00001 TABLE 1 Results of the smoke panel tests of the
different fragrant tobacco leaves after SFE extraction Tobacco
leaves Treatment Flavor Remark Guizhou Untreated Intermediate
Typical intermediate-fragrance fragrance tobacco leaves SFE
extraction Intermediate Plain fragrance, not clear one time
fragrance, unobvious SFE extraction Intermediate Poor fragrance two
times fragrance, unobvious Yunnan mild-fragrance Untreated Mild
fragrance Fragrant, occasionally clear tobacco leaves fragrance SFE
extraction Mild fragrance, Flat fragrance, substantially no one
time unobvious fragrance SFE extraction Mild fragrance Poor
fragrance two times unobvious He'nan Untreated Strong fragrance
Typical strong-fragrance SFE extraction Intermediate Tenuous
fragrance, having no tobacco leaves one time fragrance feature SFE
extraction Intermediate Poor fragrance two times fragrance,
obvious
[0042] The results of the smoke panel tests indicate that, with
one-time extraction by adopting the method of the present
invention, the fragrances of the intermediate-fragrance tobacco
leaves and the mild-fragrance tobacco leaves are not obvious, and
the strong fragrance turns into the intermediate fragrance, and all
the tobacco leaves lose the original fragrance features. It is
indicated that the fragrant ingredients in the tobacco leaves can
be substantially extracted through one-time SFE extraction method
of the present invention.
Embodiment 3
[0043] In order to study the repeatability of the method of the
present invention, by adopting the extraction method in Embodiment
1, certain fragrant tobacco leaf samples were subjected to
continuous five-time supercritical CO.sub.2 fluid extraction, and
the extract of the tobacco leaves obtained by each extraction was
subjected to gas chromatography/mass spectrometry analysis. The
analysis results are shown in FIG. 5. The detection results show
that the method of the present invention has good repeatability and
high reliability.
Embodiment 4
[0044] Key fragrant ingredients in Shandong intermediate-fragrance
tobacco leaves, Anhui strong-fragrance tobacco leaves, Hu'nan
strong-fragrance tobacco leaves, and Fujian mild-fragrance tobacco
leaves were each extracted using the following method, which
includes the following steps:
[0045] 1) cutting tobacco leaves into shreds of 0.8 mm to 1.2
mm;
[0046] 2) placing 7.5 g tobacco shreds into an extraction vessel
with supercritical CO.sub.2 fluid;
[0047] 3) setting the static extraction pressure and the extraction
temperature of the supercritical CO.sub.2 fluid to 250 bar and
55.degree. C.;
[0048] 4) performing static extraction on the tobacco shreds for 20
min under a static extraction condition;
[0049] 5) setting the CO.sub.2 dynamic extraction flow rate to 2.0
L/min;
[0050] 6) performing dynamic extraction on the tobacco shreds for
15 min under a dynamic extraction condition;
[0051] 7) collecting the supercritical CO.sub.2 fluid extract by
using a clean glass bottle, and after complete evaporation of
CO.sub.2, adding 2 mL anhydrous ethanol to dissolve the extract for
analysis of the ingredients of the extract.
[0052] The ethanol solutes, obtained through supercritical CO.sub.2
fluid extraction, of the four types of fragrant tobacco leaves were
analyzed through gas chromatography-mass spectrometry. The analysis
results are substantially the same as those in Embodiment 1.
Embodiment 5
[0053] Key fragrant ingredients in Heilongjiang
intermediate-to-mild fragrance tobacco leaves, Sichuan
mild-to-intermediate fragrance tobacco leaves, Jiangxi
intermediate-to-strong fragrance tobacco leaves, He'nan
strong-to-intermediate fragrance tobacco leaves, Yunnan
strong-over-mild fragrance tobacco leaves, and He'nan
mild-over-strong fragrance tobacco leaves were each extracted using
the following method, which includes the following steps:
[0054] 1) cutting tobacco leaves into shreds of 0.8 mm to 1.2
mm;
[0055] 2) placing 5 g tobacco shreds into an extraction vessel with
supercritical CO.sub.2 fluid;
[0056] 3) setting the static extraction pressure and the extraction
temperature of the supercritical CO.sub.2 fluid to 320 bar and
45.degree. C.;
[0057] 4) performing static extraction on the tobacco shreds for 25
min under a static extraction condition;
[0058] 5) setting the CO.sub.2 dynamic extraction flow rate to 3.5
L/min;
[0059] 6) performing dynamic extraction on the tobacco shreds for
10 min under a dynamic extraction condition;
[0060] 7) collecting the supercritical CO.sub.2 fluid extract by
using a clean glass bottle, and after complete evaporation of
CO.sub.2, adding 3 mL anhydrous ethanol to dissolve the extract for
analysis of the ingredients of the extract.
[0061] The ethanol solutes, obtained through supercritical CO.sub.2
fluid extraction, of the six types of fragrant tobacco leaves were
analyzed through gas chromatography-mass spectrometry. The analysis
results are substantially the same as those in Embodiment 1.
* * * * *