U.S. patent application number 13/733562 was filed with the patent office on 2013-08-15 for intermediates and methods for making zearalenone macrolide analogs.
This patent application is currently assigned to EISAI R&D MANAGEMENT CO., LTD.. The applicant listed for this patent is EISAI R&D MANAGEMENT CO., LTD.. Invention is credited to Roch Boivin, Silvio A. Campagna, Hong Du, Francis G. Fang, Thomas Horstmann, Charles-Andre Lemelin, Jing Li, Pamela McGuinness, Xiang Niu, Matthew J. Schnaderbeck, Kevin (kuo-ming) Wu, Xiaojie (jeff) Zhu.
Application Number | 20130211103 13/733562 |
Document ID | / |
Family ID | 40445478 |
Filed Date | 2013-08-15 |
United States Patent
Application |
20130211103 |
Kind Code |
A1 |
Boivin; Roch ; et
al. |
August 15, 2013 |
INTERMEDIATES AND METHODS FOR MAKING ZEARALENONE MACROLIDE
ANALOGS
Abstract
Disclosed herein are methods and intermediates useful in the
preparation of macrolides, e.g., compounds of formula (IV)
##STR00001## wherein R.sub.1-R.sub.12 are as defined herein.
Inventors: |
Boivin; Roch; (North
Chelmsford, MA) ; Campagna; Silvio A.; (Candia,
NH) ; Du; Hong; (North Andover, MA) ; Fang;
Francis G.; (Andover, MA) ; Horstmann; Thomas;
(Boston, MA) ; Lemelin; Charles-Andre; (North
Chelmsford, MA) ; Li; Jing; (College Station, TX)
; McGuinness; Pamela; (Methuen, MA) ; Niu;
Xiang; (Watertown, MA) ; Schnaderbeck; Matthew
J.; (Methuen, MA) ; Wu; Kevin (kuo-ming);
(Acton, MA) ; Zhu; Xiaojie (jeff); (Andover,
MA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
EISAI R&D MANAGEMENT CO., LTD.; |
|
|
US |
|
|
Assignee: |
EISAI R&D MANAGEMENT CO.,
LTD.
Tokyo
JP
|
Family ID: |
40445478 |
Appl. No.: |
13/733562 |
Filed: |
January 3, 2013 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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12746672 |
Oct 27, 2010 |
8350059 |
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PCT/US2008/013498 |
Dec 8, 2008 |
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13733562 |
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61012411 |
Dec 7, 2007 |
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61012409 |
Dec 7, 2007 |
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61012408 |
Dec 7, 2007 |
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61080048 |
Jul 11, 2008 |
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Current U.S.
Class: |
549/270 ;
549/451 |
Current CPC
Class: |
C07D 317/22 20130101;
C07D 493/04 20130101; C07D 317/24 20130101; C07D 319/08 20130101;
C07D 317/30 20130101; C07D 313/00 20130101; Y02P 20/55
20151101 |
Class at
Publication: |
549/270 ;
549/451 |
International
Class: |
C07D 313/00 20060101
C07D313/00; C07D 317/30 20060101 C07D317/30 |
Claims
1. A method for making a compound of formula (VII): ##STR00138##
wherein R.sub.1 and R.sub.2 are each independently selected from
the group consisting of hydrogen, C.sub.1-6 alkyl, C.sub.3-6
unconjugated alkenyl and C.sub.3-6 unconjugated alkynyl: R.sub.3 is
a first aromatic ring containing oxygen protecting group; R.sub.5
and R.sub.6 are each independently selected from the group
consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
R.sub.11 and R.sub.12 are each independently selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; or R.sub.11 and R.sub.12 are taken together to form a 5
membered heterocyclyldiyl of structure (a): ##STR00139## R.sub.13
is a second aromatic ring containing oxygen protecting group; and
wherein R.sub.19 and R.sub.20 are each independently selected from
the group consisting of hydrogen, C.sub.1-6 alkyl, C.sub.1-6
haloalkyl, C.sub.1-6 alkoxy and phenyl, or R.sub.19 and R.sub.20
together represent a fluorenyl moiety of structure (b):
##STR00140## comprising reacting a compound of formula (I):
##STR00141## wherein R.sub.4 is selected from the group consisting
of hydrogen and a base stable oxygen protecting group; with a
compound of formula (II): ##STR00142## wherein X is a halogen;
under suitable basic conditions, such that a compound of formula
(V) is formed: ##STR00143## wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; R.sub.3 is selected from the group consisting
of hydrogen and a base stable oxygen protecting group; R.sub.5 and
R.sub.6 are each independently selected from the group consisting
of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6
alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl,
wherein the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; R.sub.11 and R.sub.12 are
each independently selected from the group consisting of hydrogen
and a base stable oxygen protecting group; or R.sub.11 and R.sub.12
are taken together to form a 5 membered heterocyclyldiyl of
structure (a): ##STR00144## wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b): ##STR00145## and R.sub.13 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
and converting the compound of formula (V) into a compound of
formula (VII).
2. The method of claim 1, wherein the suitable basic conditions
comprise a base selected from the group consisting of a C.sub.1-6
alkyl lithium, a potassium C.sub.1-6 alkoxide, a potassium
C.sub.4-6 t-alkoxide, sodium hydroxide, sodium hydride, ammonia,
dimethylsulfoxide sodium salt and sodium
hexamethyldisilylamide.
3. The method of claim 2, wherein the base comprises a C.sub.1-6
alkyl lithium.
4. The method of claim 1, wherein the compound of formula (V) is
produced in substantially pure form without the use of
chromatography in the production of the compound of formula
(V).
5. The method of claim 1, wherein the compound of formula (VII) is
crystalline.
6-11. (canceled)
12. A method for making a compound of formula (IV): ##STR00146##
wherein R.sub.1 and R.sub.2 are each independently selected from
the group consisting of hydrogen, C.sub.1-6 alkyl, C.sub.3-6
unconjugated alkenyl and C.sub.3-6 unconjugated alkynyl; R.sub.5
and R.sub.6 are each independently selected from the group
consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
R.sub.7 is selected from the group consisting of hydrogen and
--OR.sub.a wherein R.sub.a is hydrogen or a base stable oxygen
protecting group; R.sub.8 is selected from the group consisting of
hydrogen and --OR.sub.g wherein R.sub.g is hydrogen or a base
stable oxygen protecting group; R.sub.9 is selected from the group
consisting of hydrogen, halogen, --OR.sub.b, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl, --SR.sub.d and --NR.sub.eR.sub.f wherein
R.sub.b is hydrogen or a base stable oxygen protecting group,
wherein R.sub.d is selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl and wherein R.sub.e and R.sub.f are
each independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl or a base stable nitrogen
protecting group; R.sub.10 is selected from the group consisting of
hydrogen, halogen, --OR.sub.c, C.sub.1-6 alkyl, C.sub.3-6
unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6
haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.c is hydrogen or a
base stable oxygen protecting group; and R.sub.11 and R.sub.12 are
each independently selected from the group consisting of hydrogen
and a base stable oxygen protecting group; or R.sub.11 and R.sub.12
are taken together to form a 5 membered heterocyclyldiyl of
structure (a): ##STR00147## wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b): ##STR00148## comprising reacting a compound of
formula (I): ##STR00149## wherein R.sub.3 is selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; and R.sub.4 is selected from the group consisting of
hydrogen and a base stable oxygen protecting group; with a compound
of formula (II): ##STR00150## wherein X is a halogen; and R.sub.13
is selected from the group consisting of hydrogen and a base stable
oxygen protecting group; under suitable basic conditions to form a
compound of formula (V): ##STR00151## converting the compound of
formula (V) into a compound of formula (VII): ##STR00152## wherein
R.sub.1 and R.sub.2 are each independently selected from the group
consisting of hydrogen, C.sub.1-6 alkyl, C.sub.3-6 unconjugated
alkenyl and C.sub.3-6 unconjugated alkynyl: R.sub.3 is a first
aromatic ring containing oxygen protecting group; R.sub.5 and
R.sub.6 are each independently selected from the group consisting
of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6
alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl,
wherein the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; R.sub.11 and R.sub.12 are
each independently selected from the group consisting of hydrogen
and a base stable oxygen protecting group; or R.sub.11 and R.sub.12
are taken together to form a 5 membered heterocyclyldiyl of
structure (a): ##STR00153## R.sub.13 is a second aromatic ring
containing oxygen protecting group; and wherein R.sub.19 and
R.sub.20 are each independently selected from the group consisting
of hydrogen, C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy
and phenyl, or R.sub.19 and R.sub.20 together represent a fluorenyl
moiety of structure (b): ##STR00154## and converting the compound
of formula (VII) to a compound of formula (V), wherein R.sub.13 is
H; and reacting the compound of formula (V) with a compound of
formula (III): ##STR00155## wherein Y is a halogen or
--O--SO.sub.2CF.sub.3; under suitable basic conditions, such that a
compound of formula (IV) is formed.
13. (canceled)
14. The method of claim 12, wherein the compound of formula (IV) is
produced in substantially pure form without the use of
chromatography in the production of the compound of formula
(IV).
15. (canceled)
16. The method of claim 12, wherein R.sub.1 is hydrogen.
17. The method of claim 12, wherein R.sub.2 is hydrogen.
18. The method of claim 12, wherein R.sub.5 is selected from the
group consisting of hydrogen and C.sub.1-6 alkyl.
19. The method of claim 12, wherein R.sub.6 is selected from the
group consisting of hydrogen and C.sub.1-6 alkyl.
20. The method of claim 12, wherein R.sub.5 is hydrogen or
methyl.
21. The method of claim 12, wherein R.sub.6 is hydrogen or
methyl.
22. The method of claim 12, wherein R.sub.7 is hydrogen or
hydroxyl.
23. The method of claim 12, wherein R.sub.8 is hydrogen or
hydroxyl.
24. The method of claim 12, wherein R.sub.9 is --NR.sub.eR.sub.f
and wherein R.sub.e and R.sub.f are each independently hydrogen,
C.sub.1-6 alkyl, or a base stable nitrogen protecting group.
25. The method of claim 24, wherein R.sub.e is C.sub.1-6 alkyl and
R.sub.f is hydrogen or a base stable nitrogen protecting group.
26. The method of claim 24, wherein R.sub.e is methyl or ethyl.
27. The method of claim 12, wherein R.sub.10 is hydrogen.
28. The method of claim 12, wherein R.sub.11 and R.sub.12 are taken
together to form a 5 membered heterocyclyldiyl of structure (a):
##STR00156## wherein R.sub.19 and R.sub.20 are each independently
selected from the group consisting of C.sub.1-6 alkyl.
29. (canceled)
30. The method of claim 12, wherein the compound of formula (VII)
has the structure: ##STR00157## wherein R.sub.3 is selected from
benzoyl or benzyl, wherein the benzoyl or benzyl are each
substituted with 0, 1, 2 or 3 substituents independently selected
from --OH, --O(C.sub.1-4alkyl), --NH.sub.2, --NH(C.sub.1-4alkyl),
--N(C.sub.1-4alkyl).sub.2, amides, --OCO(C.sub.1-4alkyl) and
(C.sub.1-4alkyl); R.sub.13 is ##STR00158## and wherein R.sub.14,
R.sub.15, R.sub.16, R.sub.17, and R.sub.18 are each independently
selected from the group consisting of H, NO.sub.2,
--NH.sub.3.sup.+, --COH, --CO(C.sub.1-4 alkyl), --COCl, --COOH,
--COO(C.sub.1-4 alkyl), --NR.sub.3.sup.+, --SO.sub.3H, nitrile,
--CF.sub.3 and halogen, wherein at least one of R.sub.14, R.sub.15,
R.sub.16, R.sub.17, and R.sub.18 is selected from the group
consisting of NO.sub.2, --NH.sub.3.sup.+, --COH, --CO(C.sub.1-4
alkyl), --COCl, --COOH, --COO(C.sub.1-4 alkyl), --NR.sub.3.sup.+,
--SO.sub.3H, nitrile, --CF.sub.3 and halogen and the other of
R.sub.14, R.sub.15, R.sub.16, R.sub.17, and R.sub.18 are
hydrogen.
31. The method of claim 12 wherein the compound of formula (VII)
is: ##STR00159##
Description
RELATED APPLICATIONS
[0001] This application claims priority to U.S. application Ser.
No. 12/746,672, filed on Dec. 8, 2008, which is hereby incorporated
herein by reference in its entirety.
BACKGROUND OF THE INVENTION
[0002] Macrocyclic compounds, e.g., zearalenone-like macrolides
such as F152 (LL-Z1640-2), have advantageous biological properties.
For example, F152 and certain isomers
##STR00002##
thereof inhibit the phosphorylating enzyme Map/Erk kinase (MEK).
Moreover, derivatives of F152 also have exhibited activity as
tyrosine kinase inhibitors, inhibitors of other protein kinases,
e.g., MEK1, inhibitors of NF-.kappa.B activation, and inhibitors
AP-1 activation, to name a few. Often, however, F152 and
derivatives thereof are obtained by fermentation techniques and
modifications to the natural product and thus were limited in the
number and types of derivatives that could be prepared and
evaluated for biological activity.
[0003] Chemical synthesis of F152 and derivatives have also been
disclosed (see, e.g., WO 03/076424), however, such syntheses are
often complex and have many chromatographic purification steps in
order to remove impurities.
BRIEF SUMMARY OF THE INVENTION
[0004] The present invention is based, at least in part, on the
discovery of new methods and intermediates for the preparation of
macrolides. Without wishing to be bound by any particular theory,
it is believed that such intermediates may be useful, e.g., in
providing purification points in the synthesis, thus decreasing or
even removing the need for costly and time-consuming
chromatographic steps up to that particular purification point.
Without wishing to be bound by any particular theory, it is also
believed that these new methods may be useful in providing
compositions of macrolides having increased purity and increased
yield in comparison with conventional methods.
[0005] Accordingly, in some embodiments, the present invention is
directed to methods for making a compound of formula (V):
##STR00003## [0006] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0007] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0008] R.sub.5 and R.sub.6 are each independently selected from the
group consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
[0009] R.sub.11 and R.sub.12 are each independently selected from
the group consisting of hydrogen and a base stable oxygen
protecting group; or R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
[0009] ##STR00004## [0010] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
##STR00005##
[0010] and [0011] R.sub.13 is selected from the group consisting of
hydrogen and a base stable oxygen protecting group; [0012]
comprising reacting a compound of formula (I):
[0012] ##STR00006## [0013] wherein R.sub.4 is selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; [0014] with a compound of formula (II):
[0014] ##STR00007## [0015] wherein X is a halogen; [0016] under
suitable conditions, such that compound of formula (V) is
formed.
[0017] In some embodiments the compound of formula (V) is a
compound according to (Vb):
##STR00008## [0018] and wherein the compound of formula (I) is a
compound according to formula (Ib):
##STR00009##
[0019] In some embodiments, the suitable conditions are suitable
basic conditions, e.g., conditions including, but not limited to
the presence of a base selected from the group consisting of a
C.sub.1-6 alkyl lithium, a potassium C.sub.1-6 alkoxide, a
potassium C.sub.4-6 t-alkoxide, sodium hydroxide, sodium hydride,
ammonia, dimethylsulfoxide sodium salt and sodium
hexamethyldisilylamide. In some embodiments, the suitable basic
conditions include a C.sub.1-6 alkyl lithium base.
[0020] In some embodiments, the compound of formula (V) is produced
in substantially pure form without the use of chromatography in the
production of the compound of formula (V).
[0021] In other embodiments, the present invention is directed to
methods for making an alpha-enhanced composition comprising a
compound of formula (V):
##STR00010## [0022] comprising reacting a compound of formula
(I):
[0022] ##STR00011## [0023] with a compound of formula II:
[0023] ##STR00012## [0024] under suitable basic conditions, such
that an alpha-enhanced composition comprising a compound of formula
(V) is formed.
[0025] In some embodiments, the suitable basic conditions include a
base selected from the group consisting of a C.sub.1-6 alkyl
lithium, a potassium C.sub.1-6 alkoxide, a potassium C.sub.4-6
t-alkoxide, sodium hydroxide, sodium hydride, ammonia,
dimethylsulfoxide sodium salt and sodium hexamethyldisilylamide. In
some embodiments, the suitable basic conditions include a C.sub.1-6
alkyl lithium base.
[0026] In some embodiments, R.sub.1 is hydrogen. In some
embodiments, R.sub.2 is hydrogen. In some embodiments, R.sub.5 is
selected from the group consisting of hydrogen and C.sub.1-6 alkyl,
e.g., hydrogen or methyl. In some embodiments, R.sub.6 is selected
from the group consisting of hydrogen and C.sub.1-6 alkyl, e.g.,
hydrogen or methyl. In some embodiments, R.sub.11 and R.sub.12 are
taken together to form a 5 membered heterocyclyldiyl of structure
(a):
##STR00013## [0027] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of [0028]
C.sub.1-6 alkyl. In some embodiments, the compound of formula (V)
is crystalline.
[0029] In still other embodiments, the present invention is
directed to methods for making a compound of formula (VI):
##STR00014## [0030] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0031] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0032] R.sub.4 is selected from the group consisting of hydrogen
and a base stable oxygen protecting group; [0033] R.sub.8 is
selected from the group consisting of hydrogen and --OR.sub.g
wherein R.sub.g is hydrogen or a base stable oxygen protecting
group, [0034] R.sub.9 is selected from the group consisting of
hydrogen, halogen, --OR.sub.6, C.sub.1-6 alkyl, C.sub.3-6
unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6
haloalkyl, --SR.sub.d and [0035] --NR.sub.eR.sub.f wherein R.sub.b
is hydrogen or a base stable oxygen protecting group, wherein
R.sub.d is selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl and wherein R.sub.e and R.sub.f are
each independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl or a base stable nitrogen
protecting group; [0036] R.sub.10 is selected from the group
consisting of hydrogen, halogen, --OR.sub.c, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.c is
hydrogen or a base stable oxygen protecting group; and [0037]
R.sub.11 and R.sub.12 are each independently selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; or R.sub.11 and R.sub.12 are taken together to form a 5
membered heterocyclyldiyl of structure (a):
[0037] ##STR00015## [0038] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
[0038] ##STR00016## [0039] comprising reacting a compound of
formula (I):
[0039] ##STR00017## [0040] with a compound of formula (III):
[0040] ##STR00018## [0041] wherein Y is a halogen or
--O--SO.sub.2CF.sub.3, [0042] under suitable conditions, such that
a compound of formula (VI) is formed.
[0043] In some embodiments, the compound of formula (VI) is a
compound according to (VIb):
##STR00019## [0044] and the compound of formula (I) is a compound
according to formula (Ib):
##STR00020##
[0045] In some embodiments, the suitable conditions are suitable
basic conditions. In some embodiments, the reacting step is
catalyzed by a palladium catalyst.
[0046] In some embodiments, the compound of formula (VI) is
produced in substantially pure form without the use of
chromatography in the production of the compound of formula
(VI).
[0047] In some embodiments, R.sub.1 is hydrogen. In some
embodiments, R.sub.2 is hydrogen. In some embodiments, R.sub.8 is
hydrogen or hydroxyl. In some embodiments, R.sub.9 is
--NR.sub.eR.sub.f and wherein R.sub.e and R.sub.f are each
independently hydrogen, C.sub.1-6 alkyl, or a base stable nitrogen
protecting group. In some embodiments, R.sub.10 is hydrogen.
[0048] In still other embodiments, the present invention is
directed to methods for making a compound of formula (IV):
##STR00021## [0049] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0050] R.sub.5 and R.sub.6 are each
independently selected from the group consisting of hydrogen,
halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl, wherein
the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; [0051] R.sub.7 is selected
from the group consisting of hydrogen and --OR.sub.a wherein
R.sub.a is hydrogen or a base stable oxygen protecting group;
[0052] R.sub.8 is selected from the group consisting of hydrogen
and --OR.sub.g wherein R.sub.g is hydrogen or a base stable oxygen
protecting group; [0053] R.sub.9 is selected from the group
consisting of hydrogen, halogen, --OR.sub.b, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl, --SR.sub.d and [0054] --NR.sub.eR.sub.f
wherein R.sub.b is hydrogen or a base stable oxygen protecting
group, wherein R.sub.d is selected from the group consisting of
hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl and wherein R.sub.e and R.sub.f are
each independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl or a base stable nitrogen
protecting group; [0055] R.sub.10 is selected from the group
consisting of hydrogen, halogen, --OR.sub.c, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.c is
hydrogen or a base stable oxygen protecting group; and [0056]
R.sub.11 and R.sub.12 are each independently selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; or R.sub.11 and R.sub.12 are taken together to form a 5
membered heterocyclyldiyl of structure (a):
[0056] ##STR00022## [0057] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
[0057] ##STR00023## [0058] comprising combining a compound of
formula (I):
##STR00024##
[0059] wherein [0060] R.sub.3 is selected from the group consisting
of hydrogen and a base stable oxygen protecting group; and [0061]
R.sub.4 is selected from the group consisting of hydrogen and a
base stable oxygen protecting group; [0062] with a compound of
formula (II):
##STR00025##
[0063] wherein [0064] X is a halogen; and [0065] R.sub.13 is
selected from the group consisting of hydrogen and a base stable
oxygen protecting group; [0066] and a compound of formula
(III):
##STR00026##
[0067] wherein Y is a halogen or --O--SO.sub.2CF.sub.3, [0068]
under suitable conditions, such that an alpha-intermediate and a
compound of formula (IV) are formed.
[0069] In some embodiments, the alpha-intermediate is a compound of
formula (V):
##STR00027## [0070] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0071] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0072] R.sub.5 and R.sub.6 are each independently selected from the
group consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
[0073] R.sub.11 and R.sub.12 are each independently selected from
the group consisting of hydrogen and a base stable oxygen
protecting group; or R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
[0073] ##STR00028## [0074] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
##STR00029##
[0074] and [0075] R.sub.13 is selected from the group consisting of
hydrogen and a base stable oxygen protecting group.
[0076] In some embodiments, the alpha-intermediate is a compound of
formula (VI):
##STR00030## [0077] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0078] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0079] R.sub.4 is selected from the group consisting of hydrogen
and a base stable oxygen protecting group; [0080] R.sub.8 is
selected from the group consisting of hydrogen and --OR.sub.g
wherein R.sub.g is hydrogen or a base stable oxygen protecting
group, [0081] R.sub.9 is selected from the group consisting of
hydrogen, halogen, --OR.sub.6, C.sub.1-6 alkyl, C.sub.3-6
unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6
haloalkyl, --SR.sub.d and [0082] --NR.sub.eR.sub.f wherein R.sub.b
is hydrogen or a base stable oxygen protecting group, wherein
R.sub.d is selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl and wherein R.sub.e and R.sub.f are
each independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl or a base stable nitrogen
protecting group; [0083] R.sub.10 is selected from the group
consisting of hydrogen, halogen, --OR.sub.c, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.c is
hydrogen or a base stable oxygen protecting group; and [0084]
R.sub.11 and R.sub.12 are each independently selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; or R.sub.11 and R.sub.12 are taken together to form a 5
membered heterocyclyldiyl of structure (a):
[0084] ##STR00031## [0085] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
##STR00032##
[0086] In some embodiments, the compound of formula (IV) is a
compound according to (IVa):
##STR00033## [0087] and the compound of formula (II) is a compound
according to formula (IL):
##STR00034##
[0088] In some embodiments, the compound of formula (I) is
crystalline.
[0089] In some embodiments, the compound of formula (IV) is
produced in substantially pure form without the use of
chromatography in the production of the compound of formula
(IV).
[0090] In yet other embodiments, the present invention is directed
to methods for making a compound of formula (IV):
##STR00035## [0091] comprising reacting a compound of formula
(I):
[0091] ##STR00036## [0092] with a compound of formula (II):
[0092] ##STR00037## [0093] under suitable basic conditions to form
a compound of formula (V):
[0093] ##STR00038## [0094] and reacting the compound of formula (V)
with a compound of formula (III):
[0094] ##STR00039## [0095] under suitable basic conditions, such
that a compound of formula (IV) is formed.
[0096] In other embodiments, the present invention is directed to
methods for making a compound of formula (IV):
##STR00040## [0097] comprising reacting a compound of formula
(I):
[0097] ##STR00041## [0098] with a compound of formula (III):
[0098] ##STR00042## [0099] under suitable basic conditions to form
a compound of formula (VI):
[0099] ##STR00043## [0100] and reacting the compound of formula
(VI) with a compound of formula (II):
[0100] ##STR00044## [0101] under suitable basic conditions, such
that a compound of formula (IV) is formed.
[0102] Values for R.sub.1-R.sub.13, X and Y are as described
herein. In some embodiments, R.sub.1 is hydrogen. In some
embodiments, R.sub.2 is hydrogen. In some embodiments, R.sub.5 is
selected from the group consisting of hydrogen and C.sub.1-6 alkyl,
e.g., hydrogen or methyl. In some embodiments, R.sub.6 is selected
from the group consisting of hydrogen and C.sub.1-6 alkyl, e.g.,
hydrogen or methyl. In some embodiments, R.sub.7 is hydrogen or
hydroxyl. In some embodiments, R.sub.8 is hydrogen or hydroxyl. In
some embodiments, R.sub.9 is --NR.sub.eR.sub.f and R.sub.e and
R.sub.f are each independently hydrogen, C.sub.1-6 alkyl, or a base
stable nitrogen protecting group. In some embodiments, R.sub.e is
C.sub.1-6 alkyl, e.g., methyl or ethyl, and R.sub.f is hydrogen or
a base stable nitrogen protecting group. In some embodiments,
R.sub.10 is hydrogen.
[0103] In further embodiments, the present invention is directed to
methods for making a composition comprising a compound of formula
(IV):
##STR00045## [0104] comprising combining a compound of formula
(I):
[0104] ##STR00046## [0105] with a compound of formula II:
[0105] ##STR00047## [0106] and a compound of formula III:
##STR00048##
[0106] under suitable conditions, such that a composition
comprising a compound of formula (IV) is formed, wherein the
composition is purity-enhanced, yield-enhanced and/or substantially
free of organic impurities. Values for R.sub.1-R.sub.13, X and Y
are as described herein.
[0107] In some embodiments, R.sub.1 is hydrogen. In some
embodiments, R.sub.2 is hydrogen. In some embodiments, R.sub.5 is
selected from the group consisting of hydrogen and C.sub.1-6 alkyl,
e.g., hydrogen or methyl. In some embodiments, R.sub.6 is selected
from the group consisting of hydrogen and C.sub.1-6 alkyl, e.g.,
hydrogen or methyl. In some embodiments, R.sub.7 is hydrogen or
hydroxyl. In some embodiments, R.sub.8 is hydrogen or hydroxyl. In
some embodiments, R.sub.9 is --NR.sub.eR.sub.f and R.sub.e and
R.sub.f are each independently hydrogen, C.sub.1-6 alkyl, or a base
stable nitrogen protecting group. In some embodiments, R.sub.e is
C.sub.1-6 alkyl, e.g., methyl or ethyl, and R.sub.f is hydrogen or
a base stable nitrogen protecting group. In some embodiments,
R.sub.10 is hydrogen.
[0108] In some embodiments, the compound of formula (I) is
crystalline.
[0109] In some embodiments, the present invention is directed to
one or more of the intermediates described in more detail
herein.
BRIEF DESCRIPTION OF THE DRAWINGS
[0110] FIG. 1 depicts a single crystal X-ray structure, and the
corresponding chemical structure, of intermediate compound 011.
[0111] FIG. 2 depicts single crystal X-rays of intermediates 002
and 011a.
[0112] FIGS. 3-12 are 100 MHz .sup.13C NMR (A) and 400 MHz .sup.1H
NMR (B) spectra of exemplary intermediates and an exemplary final
product of the present invention in CDCl.sub.3. Specifically, FIG.
3 depicts 100 MHz 13C NMR (A) and 400 MHz 1H NMR (B) spectra of
compound 002. FIG. 4 depicts 100 MHz .sup.13C NMR (A) and 400 MHz
.sup.1H NMR (B) spectra of compound 003. FIG. 5 depicts 100 MHz
.sup.13C NMR (A) and 400 MHz .sup.1H NMR (B) spectra of compound
006. FIG. 6 depicts 100 MHz .sup.13C NMR (A) and 400 MHz .sup.1H
NMR (B) spectra of compound 007. FIG. 7 depicts 100 MHz .sup.13C
NMR (A) and 400 MHz .sup.1H NMR (B) spectra of compound 009. FIG. 8
depicts 100 MHz .sup.13C NMR (A) and 400 MHz .sup.1H NMR (B)
spectra of compound 010. FIG. 9 depicts 100 MHz .sup.13C NMR (A)
and 400 MHz .sup.1H NMR (B) spectra of compound 011. FIG. 10
depicts 100 MHz .sup.13C NMR (A) and 400 MHz .sup.1H NMR (B)
spectra of compound 012. FIG. 11 depicts 100 MHz .sup.13C NMR (A)
and 400 MHz .sup.1H NMR (B) spectra of compound 011a. FIG. 12
depicts 100 MHz .sup.13C NMR (A) and 400 MHz .sup.1H NMR (B)
spectra of compound 09a.
DETAILED DESCRIPTION OF THE INVENTION
[0113] The present invention provides methods and intermediates for
the preparation of macrolides, e.g., compounds of formula (IV)
##STR00049## [0114] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0115] R.sub.5 and R.sub.6 are each
independently selected from the group consisting of hydrogen,
halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl, wherein
the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; [0116] R.sub.7 is selected
from the group consisting of hydrogen and --OR.sub.a wherein
R.sub.a is hydrogen or a base stable oxygen protecting group;
[0117] R.sub.8 is selected from the group consisting of hydrogen
and --OR.sub.g wherein R.sub.g is hydrogen or a base stable oxygen
protecting group; [0118] R.sub.9 is selected from the group
consisting of hydrogen, halogen, --OR.sub.b, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl, --SR.sub.d and --NR.sub.eR.sub.f wherein
R.sub.b is hydrogen or a base stable oxygen protecting group,
wherein R.sub.d is selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl and wherein R.sub.e and R.sub.f are
each independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl or a base stable nitrogen
protecting group; and [0119] R.sub.10 is selected from the group
consisting of hydrogen, halogen, --OR.sub.c, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.c is
hydrogen or a base stable oxygen protecting group; and [0120]
R.sub.11 and R.sub.12 are each independently selected from the
group consisting of hydrogen and a base stable oxygen protecting
group; or R.sub.11 and R.sub.12 are taken together to form a 5
membered heterocyclyldiyl of structure (a):
[0120] ##STR00050## [0121] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
[0121] ##STR00051## [0122] and compositions comprising the
same.
DEFINITIONS
[0123] In order to more clearly and concisely describe the subject
matter of the claims, the following definitions are intended to
provide guidance as to the meaning of specific terms used
herein.
[0124] It is to be noted that the singular forms "a," "an," and
"the" as used herein include "at least one" and "one or more"
unless stated otherwise. Thus, for example, reference to "a
pharmacologically acceptable carrier" includes mixtures of two or
more carriers as well as a single carrier, and the like.
[0125] Numerous values and ranges are recited in connection with
various embodiments of the present invention, e.g., amount of a
compound of the invention present in a composition. It is to be
understood that all values and ranges which fall between the values
and ranges listed are intended to be encompassed by the present
invention unless explicitly stated otherwise. Additionally, it is
also to be understood that all numerical values listed herein are
implicitly modified by the term "about" unless specifically stated
otherwise. The term "about" as used herein in association with
parameters, ranges and amounts, means that the parameter or amount
is within .+-.1.0% of the stated parameter or amount.
[0126] As used herein, "alkyl" groups include saturated
hydrocarbons having one or more carbon atoms, including
straight-chain alkyl groups (e.g., methyl, ethyl, propyl, butyl,
pentyl, hexyl, etc.), cyclic alkyl groups (or "cycloalkyl" or
"alicyclic" or "carbocyclic" groups) (e.g., cyclopropyl,
cyclopentyl, cyclohexyl, etc.), branched-chain alkyl groups
(isopropyl, tert-butyl, sec-butyl, isobutyl, etc.), and
alkyl-substituted alkyl groups (e.g., alkyl-substituted cycloalkyl
groups and cycloalkyl-substituted alkyl groups). In certain
embodiments, a straight-chain or branched-chain alkyl group may
have 8 or fewer carbon atoms in its backbone, e.g., C.sub.1-C.sub.8
for straight-chain or C.sub.3-C.sub.8 for branched-chain. In
certain embodiments, a straight-chain or branched-chain alkyl group
may have 6 or fewer carbon atoms in its backbone, e.g.,
C.sub.1-C.sub.6 for straight-chain or C.sub.3-C.sub.6 for
branched-chain. In still other embodiments, an alkyl group includes
about 1 to 4 carbons. In other embodiments, an alkyl group includes
about 1 to 3 carbons. In yet other embodiments, an alkyl group
includes about 1 or 2 carbons. In some embodiments, preferred alkyl
groups include C.sub.1-C.sub.6 alkyl groups. The terms
"C.sub.1-C.sub.6" and "C.sub.1-6" as in "C.sub.1-C.sub.6 alkyl" and
"C.sub.1-6 alkyl" are used interchangeably to mean alkyl groups
containing 1 to 6 carbon atoms. The term "haloalkyl" means alkyl
groups wherein one or more, e.g., 1 to 3, hydrogen atoms have been
replaced with a halogen. The terms "alkenyl" and "alkynyl" refer to
unsaturated aliphatic groups analogous to alkyls, but which contain
at least one double or triple carbon-carbon bond respectively. In
complex structures, carbon chains may be branched, bridged, or
cross-linked. The terms "unconjugated alkenyl" and "unconjugated
alkynyl" respectively refer alkenyl and alkynyl groups that are not
conjugated with a portion of the core molecule.
[0127] The term "heteroalkyl group" includes straight-chain or
branched-chain structures analogous to alkyl groups in which one or
more of the carbon atoms in the chain is an element other than
carbon, for example, nitrogen, sulfur, or oxygen. The term
"heteroalkenyl group" includes straight-chain or branched-chain
structures analogous to alkenyl groups in which one or more of the
carbon atoms in the chain is an element other than carbon, for
example, nitrogen, sulfur, or oxygen. The term "heteroalkynyl
group" includes straight-chain or branched-chain structures
analogous to alkynyl groups in which one or more of the carbon
atoms in the chain is an element other than carbon, for example,
nitrogen, sulfur, or oxygen. The term "C.sub.1-6 heteroalkyl" and
"C.sub.1-C.sub.6 heteroalkyl" are used interchangeably to refer to
a moiety that has from 1-6 carbons and one or more heteroatoms.
[0128] The term "alkoxy" as used herein means an alkyl group having
an oxygen atom attached thereto. In some embodiments, alkoxy groups
include groups having 1 to about 8 carbon atoms. In other
embodiments, alkoxy groups include groups having 1 to about 6
carbon atoms. In still other embodiments, alkoxy groups include
groups having fewer than about 4 carbon atoms. In some embodiments,
preferred alkoxy groups include C.sub.1-C.sub.6 alkoxy groups.
Examples of alkoxy groups include, but are not limited to, methoxy,
ethoxy, isopropyloxy, propoxy, butoxy, and pentoxy groups. The
alkoxy groups can be straight-chain or branched.
[0129] The term "aromatic group" or "aryl group" includes
unsaturated and aromatic cyclic hydrocarbons as well as unsaturated
and aromatic heterocycles containing one or more rings. Aryl groups
include, for example C.sub.5-8 aryl groups. Aryl groups may also be
fused or bridged with alicyclic or heterocyclic rings that are not
aromatic so as to form a polycycle (e.g., tetralin).
[0130] The term "heterocyclic group" includes closed ring
structures analogous to carbocyclic groups in which one or more,
e.g., 1, 2 or 3, of the carbon atoms in the ring is an element
other than carbon, for example, nitrogen, sulfur, or oxygen.
Heterocyclic groups may be saturated or unsaturated. Additionally,
heterocyclic groups (such as pyrrolyl, pyridyl, isoquinolyl,
quinolyl, purinyl, and furyl) may or may not have aromatic
character, in which case they may be referred to as "heteroaryl" or
"heteroaromatic" groups. The term heterocyclic group includes rings
which are attached to the core structure via either a bond to one
of the heteroatoms in the ring or a bond to one of the carbons in
the ring. Exemplary heterocyclic groups include, but are not
limited to imidazolyl,
##STR00052##
[0131] The term "amine" or "amino," as used herein, refers to an
unsubstituted or substituted moiety of the formula
--NR.sub.xR.sub.y, in which R.sub.x and R.sub.y are each
independently hydrogen, alkyl, aryl, or heterocyclyl, or R.sub.x
and R.sub.y, taken together with the nitrogen atom to which they
are attached, form a cyclic moiety having from 3 to 8 atoms in the
ring. Thus, the term amino includes cyclic amino moieties such as
piperidinyl or pyrrolidinyl groups, unless otherwise stated.
[0132] The chemical moieties of the compounds of the invention,
including those groups discussed above, may be "substituted or
unsubstituted." In some embodiments, the term "substituted" means
that the moiety has substituents placed on the moiety other than
hydrogen (i.e., in most cases, replacing a hydrogen), which allow
the molecule to perform its intended function. It will be
understood that "substitution" or "substituted with" includes the
implicit proviso that such substitution is in accordance with the
permitted valence of the substituted atom and the substituent, and
that the substitution results in a stable compound, e.g., which
does not spontaneously undergo transformation such as by
rearrangement, cyclization, elimination, etc. As used herein, the
term "substituted" is meant to include all permissible substituents
of organic compounds. In a broad aspect, permissible substituents
include acyclic and cyclic, branched and unbranched, carbocyclic
and heterocyclic, aromatic and nonaromatic substituents of organic
compounds. The compounds of the present invention may have one or
more substitutions, as described herein.
[0133] When compounded chemical names, e.g., "alkylaryl,"
"aryloxy," and the like, are used herein, they are understood to
have a specific connectivity to the core of the chemical structure.
The moiety listed farthest to the right (e.g., aryl in
"alkylaryl"), is the moiety which is directly connected to the
core. Thus, an "arylalkyl" group, for example, is an alkyl group
substituted with an aryl group (e.g., phenylmethyl (i.e., benzyl)).
An "alkylaryl" moiety is an aryl group substituted with an alkyl
group (e.g., p-methylphenyl (i.e., p-tolyl)).
[0134] The conventions
##STR00053##
are used interchangeably to indicate a double bond having two
substituents, where the substituents may be either cis or
trans.
[0135] As used herein, the term "compound" is intended to mean a
substance made up of molecules that further consist of atoms. A
compound generally refers to a chemical entity, whether in the
solid, liquid or gaseous phase, and whether in a crude mixture or
purified and isolated. Compounds encompass the chemical compound
itself as well as, where applicable: amorphous and crystalline
forms of the compound, including polymorphic forms, said forms in
mixture or in isolation; free acid and free base forms of the
compound; isomers of the compound, including geometric isomers,
optical isomers, and tautomeric isomers, said optical isomers to
include enantiomers and diastereomers, chiral isomers and
non-chiral isomers, said optical isomers to include isolated
optical isomers or mixtures of optical isomers including racemic
and non-racemic mixtures; said geometric isomers to include
transoid and cisoid forms, where an isomer may be in isolated form
or in admixture with one or more other isomers; isotopes of the
compound, including deuterium- and tritium-containing compounds,
and including compounds containing radioisotopes, including
therapeutically- and diagnostically-effective radioisotopes;
multimeric forms of the compound, including dimeric, trimeric, etc.
forms; salts of the compound, including acid addition salts and
base addition salts, including organic counterions and inorganic
counterions, and including zwitterionic forms, where if a compound
is associated with two or more counterions, the two or more
counterions may be the same or different; and solvates of the
compound, including hemisolvates, monosolvates, disolvates, etc.,
including organic solvates and inorganic solvates, said inorganic
solvates including hydrates; where if a compound is associated with
two or more solvent molecules, the two or more solvent molecules
may be the same or different.
[0136] The term "protecting group" as used herein, refers to a
particular functional moiety, e.g., O, S, or N, being temporarily
blocked so that a reaction can be carried out selectively at
another reactive site in a multifunctional compound. In various
embodiments, a protecting group reacts selectively in good yield to
give a protected substrate that is stable to the projected
reactions; is selectively removed in good yield by readily
available, preferably nontoxic reagents that do not attack the
other functional groups; forms an easily separable derivative (more
preferably without the generation of new stereogenic centers); and
has a minimum of additional functionality to avoid further sites of
reaction. Compatibility of the protecting groups will typically
take into consideration the reaction conditions in subsequent
steps. Thus, if basic conditions are used, a protecting group
readily cleavable by basic moieties may not be preferred.
Similarly, if acidic conditions are used, a protecting group
readily cleavable by acidic moieties may not be preferred.
Protecting groups used in various embodiments of the present
invention are described in more detail herein. Methods for removing
protecting groups are well known in the art. For example, regarding
typical oxygen protecting groups; acetyl groups can be removed
under acidic conditions or basic conditions; methoxyethoxymethyl
ether groups can be removed under acidic conditions; methoxymethyl
ether groups can be removed under acidic conditions; methoxybenzyl
ether groups can be removed under acidic conditions, by
hydrogenolysis, or by oxidation; methylthiomethyl ether groups can
be removed under acidic conditions; pivaloyl groups can be removed
under acidic conditions, basic conditions or with reducing agents;
tetrahydropyran groups can be removed under acidic conditions;
silyl ether groups (including trimethylsilyl (TMS),
tert-butyldimethylsilyl (TBDMS), and triisopropylsilyl (TIPS)
ethers) can be removed under acidic conditions or with fluoride ion
such as NaF or TBAF; and methyl ethers can be removed with TMSI in
dichloromethane or MeCN or chloroform or BBr.sub.3 in
dichloromethane. In another example, regarding typical nitrogen
protecting groups, carbobenzyloxy groups can be removed by
hydrogenolysis; tert-Butyloxycarbonyl groups can be removed with
concentrated, strong acid such as HCl or CF.sub.3COOH;
9-Fluorenylmethyloxycarbonyl groups can be removed with base such
as piperidine; benzyl groups can be removed by hydrogenolysis; and
p-methoxyphenyl (PMP) group can be removed with ammonium cerium(IV)
nitrate. As used herein, the term "base stable" protecting group
refers to a protecting group that is stable under the basic
conditions of any reaction of the compound which occurs subsequent
to protection and prior to removal of the protecting group.
Similarly, as used herein, the term "acid stable" protecting group
refers to a protecting group that is stable under the acidic
conditions of any reaction of the compound which occurs subsequent
to protection and prior to removal of the protecting group. A
skilled artisan would understand that a "base stable" protecting
group is not necessarily stable to every base, (e.g., may not be
stable to concentrated sodium hydroxide) but is stable to any bases
utilised in any reaction of the compound which occurs subsequent to
protection and prior to removal of the protecting group. Exemplary
base stable protecting groups include, but are not limited to
acetyl groups, methoxyethoxymethyl ether groups, methoxymethyl
ether groups, methoxybenzyl ether groups, methylthiomethyl ether
groups, pivaloyl groups, tetrahydropyran groups, silyl ether groups
(including trimethylsilyl (TMS), tert-butyldimethylsilyl (TBDMS),
and triisopropylsilyl (TIPS) ethers) and methyl ethers,
carbobenzyloxy groups, tert-butyloxycarbonyl groups, benzyl groups
and p-methoxyphenyl (PMP) groups. Similarly, a skilled artisan
would understand that an "acid stable" protecting group is not
necessarily stable to every acid, (e.g., may not be stable to
concentrated hydrochloric acid) but is stable to any acids utilised
in any reaction of the compound which occurs subsequent to
protection and prior to removal of the protecting group.
[0137] The term "reacting," as used herein, refers to a chemical
process or processes in which two or more reactants are allowed to
come into contact with each other to effect a chemical change or
transformation. For example, when reactant A and reactant B are
allowed to come into contact with each other to afford one or more
new chemical compound(s) C(C', C'', etc.), A is said to have
"reacted" with B to produce C.
[0138] The language "large scale" as used in the language "large
scale preparation" includes reactions which result in product in an
amount, e.g., greater than 26 g, e.g., greater than 30 g, e.g.,
greater than 35 g, e.g., greater than 40 g, e.g., greater than 45
g, e.g., greater than 50 g, e.g., greater than 60 g, e.g., greater
than 70 g, e.g., greater than 80 g, e.g., greater than 90 g, e.g.,
greater than 100 g, e.g., greater than 200 g, e.g., greater than
500 g, e.g., greater than 1 kg, e.g., greater than 2 kg, e.g.,
greater than 5 kg, e.g., greater than 10 kg, e.g., greater than 20
kg, e.g., greater than 40 kg, e.g., greater than 60 kg, e.g.,
greater than 100 kg, e.g., greater than 300 kg e.g., greater than
500 kg.
[0139] The language "alpha-enhanced" is used in reference to the
composition comprising the intermediates, e.g., compounds of
formula (V) and/or (VI), which include a higher ratio of .alpha.
isomer to .beta. isomer than previously prepared compositions. The
.alpha. and .beta. isomers of formula (V) and (VI) are shown
below.
##STR00054##
Previously prepared compositions (see, e.g., U.S. Patent
Application Publication No. 20060247448, published Nov. 2, 2006;
paragraphs [1242]-[1248]) typically have a ratio of .alpha. isomer
to .beta. isomer of about 1:2. In some embodiments, the term
"alpha-enhanced" refers compositions which include compounds of
formula (V) and/or (VI) in an .alpha. to .beta. ratio of at least
about 1:1.5. In some embodiments, the term "alpha-enhanced" refers
compositions which include compounds of formula (V) and/or (VI) in
an .alpha. to .beta. ratio of at least about 1:1, e.g., at least
about 2:1, 3:1, 4:1, 5:1, 10:1, 25:1, 50:1, 100:1, 500:1, or
1000:1. In some embodiments, the term "alpha-enhanced" refers
compositions which include compounds of formula (V) and/or (VI)
having only the a isomer. It should be noted that alpha-enhanced
compositions of the invention are not intended to be limited by
scale of the reaction that produces the compounds. Similarly the
language "alpha-intermediate" is used in reference to the
intermediates, e.g., compounds of formula (V) and/or (VI), which
represent the a isomer, as described in more detail herein.
[0140] The language "yield-enhanced" is used in reference to the
composition comprising compounds of formula (IV) synthesized using
alpha-enhanced intermediate compositions (see, e.g., U.S. Patent
Application Publication No. 20060247448). These compositions have a
higher yield of target compound (e.g., compounds of formula (IV))
in comparison with products synthesized using non-alpha-enhanced
intermediate compositions. In some embodiments, the term
"yield-enhanced" refers to at least 5% additional yield versus a
non-alpha-enhanced composition. In some embodiments, the term
"yield-enhanced" refers to at least 10%, 20%, 30%, 40%, 50%, 60%,
70%, 80%, 90%, 100% or 200% additional yield versus a
non-alpha-enhanced composition. It should be noted that the
percentages used in the context of percent yield is intended to
describe percentages relative to the yield of the final product
(i.e., weight by weight, w/w). It should also be noted that
yield-enhanced compositions of the invention are not intended to be
limited by scale of the reaction that produces the compounds.
[0141] The language "purity-enhanced" is used in reference to the
composition comprising the final product, e.g., a composition
comprising a compound of formula (IV) as the therapeutic agent,
which is significantly free of impurities, e.g., impurities that
are side-products of the reaction or residual starting material
that would be considered unsuitable for administration to a
subject, e.g., a human, or preferentially omitted by a skilled
artisan from a pharmaceutical composition prepared for
administration to a subject. In some embodiments, the term
"purity-enhanced" refers to the purity of products synthesized
using alpha-enhanced intermediate compositions versus products
synthesized using intermediate compositions which are not
alpha-enhanced (see, e.g., U.S. Patent Application Publication No.
20060247448). It should be noted that purity-enhanced compositions
of the invention are not intended to be limited by scale of the
reaction that produces the compounds.
[0142] The language "free of" is used herein, in reference to a
composition of the present invention which is significantly,
substantially or completely lacking a referenced item, for example,
an impurity (such as p-anisaldehyde), which has been introduced
into the reaction through the synthetic process. For example, in
certain embodiments, the language "free of" is not intended to
encompass impurities, for example, residual sodium, which has been
introduced through environmental factors rather than through the
synthetic process.
[0143] The language "significantly free of" as used in the language
"significantly free of impurities" characterizes the presence of
impurities, e.g., dimers, acetonide elimination products, compounds
where an allylic methyl is eliminated, etc., in a final product,
e.g., a composition comprising a compound of formula (IV) as the
therapeutic agent, in an amount that is less than or equal to 10%,
e.g., less than or equal to 9%, e.g., less than or equal to 8%,
e.g., less than or equal to 7%, e.g., less than or equal to 6%,
e.g., less than or equal to 5%, e.g., less than or equal to 4%,
e.g., less than or equal to 3%, e.g., less than or equal to 2%,
e.g., less than or equal to 1.5%, e.g., less than or equal to 1%,
e.g., less than or equal to 0.5%, e.g., less than or equal to 0.4%,
e.g., less than or equal to 0.3%, e.g., less than or equal to 0.2%,
e.g., less than or equal to 0.175%, e.g., less than or equal to
0.15%, e.g., less than or equal to 0.125%, e.g., less than or equal
to 0.1%, e.g., less than or equal to 0.75%, e.g., less than or
equal to 0.5%, e.g., less than or equal to 0.25%, and e.g., 0%. In
specific embodiments the purity-enhanced compositions of the
present invention are significantly free of organic impurities,
e.g., impurities composed, at least partially, of carbon atoms,
e.g., p-anisaldehyde (or any other of possible intermediates or
elimination products shown herein). The language "substantially
free of" as used in the language "substantially free of impurities"
characterizes the presence of impurities, e.g., dimers, acetonide
elimination products, compounds where an allylic methyl is
eliminated, etc., in a final product, e.g., a composition
comprising a compound of formula (IV) as the therapeutic agent, in
an amount that is less than or equal to 5%, e.g., less than or
equal to 4%, e.g., less than or equal to 3%, e.g., less than or
equal to 2%, e.g., less than or equal to 1.5%, e.g., less than or
equal to 1%, e.g., less than or equal to 0.5%, e.g., less than or
equal to 0.4%, e.g., less than or equal to 0.3%, e.g., less than or
equal to 0.2%, e.g., less than or equal to 0.175%, e.g., less than
or equal to 0.15%, e.g., less than or equal to 0.125%, e.g., less
than or equal to 0.1%, e.g., less than or equal to 0.75%, e.g.,
less than or equal to 0.5%, e.g., less than or equal to 0.25%, and
e.g., 0%. The language "substantially pure" as used herein, also
refers to the presence of impurities of less than or equal to 5%,
e.g., less than or equal to 4%, e.g., less than or equal to 3%,
e.g., less than or equal to 2%, e.g., less than or equal to 1.5%,
e.g., less than or equal to 1%, e.g., less than or equal to 0.5%,
e.g., less than or equal to 0.4%, e.g., less than or equal to 0.3%,
e.g., less than or equal to 0.2%, e.g., less than or equal to
0.175%, e.g., less than or equal to 0.15%, e.g., less than or equal
to 0.125%, e.g., less than or equal to 0.1%, e.g., less than or
equal to 0.75%, e.g., less than or equal to 0.5%, e.g., less than
or equal to 0.25%, and e.g., 0%. It should be noted that the
percentages used in the context of percentage of impurities is
intended to describe percentages relative to the weight of the
final product, e.g., pharmaceutical composition (i.e., weight by
weight, w/w). In some embodiments, the percent impurities are
measured as area % impurity (e.g., by HPLC).
Methods of the Invention
[0144] The present invention is directed, at least in part, to
novel methods for synthesizing macrolides. In some embodiments, the
present invention is directed to large scale preparation of
macrolides. Schemes 1 and 2 depict exemplary methods for
synthesizing macrolides of the present invention. Scheme 1 is a
schematic showing the protection and ring opening of a ribose
molecule, followed by coupling to a triphenylphosphonium salt,
coupling to a bicyclic triflate and ring closing to form the
macrolide.
##STR00055##
[0145] Additionally, the same building blocks may be employed as in
Scheme 2, which is a schematic showing the protection and ring
opening of a ribose molecule, followed by coupling to a bicyclic
triflate, coupling to a triphenylphosphonium salt and ring closing
to form the macrolide.
##STR00056##
[0146] Accordingly, in some aspects, the present invention is
directed to methods for making a compound of formula (V):
##STR00057## [0147] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0148] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0149] R.sub.5 and R.sub.6 are each independently selected from the
group consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
[0150] R.sub.11 and R.sub.12 are each independently selected from
the group consisting of hydrogen and a base stable oxygen
protecting group; or R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
[0150] ##STR00058## [0151] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
##STR00059##
[0151] and [0152] R.sub.13 is selected from the group consisting of
hydrogen and a base stable oxygen protecting group.
[0153] Compounds of formula (V) are synthesized by reacting a
compound of formula I:
##STR00060## [0154] wherein R.sub.4 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
with a compound of formula (II):
[0154] ##STR00061## [0155] wherein X is a halogen; under suitable
conditions, such that a compound of formula (V) is formed.
[0156] In some embodiments, suitable conditions for forming a
compound of formula (V) are suitable basic conditions. In some
embodiments, suitable conditions for forming a compound of formula
(V) include the use of a base, e.g., a C.sub.1-6 alkyl lithium, a
potassium C.sub.1-6 t-alkoxide, sodium hydroxide, sodium hydride,
ammonia, dimethylsulfoxide sodium salt and sodium
hexamethyldisilylamide. In some embodiments, suitable conditions
for forming a compound of formula (V) include the use of a
C.sub.1-6 alkyl lithium base such as butyl lithium.
[0157] The compounds of formula (I) and formula (II) can have
varying stereochemistries at each chiral carbon. In preferred
embodiments, the compounds of formula (I) and formula (II) have a
specific stereochemistry at each chiral carbon. Accordingly, the
resulting compound of formula (V) can have varying
stereochemistries at each chiral carbon. For example, in some
embodiments, the compound of formula (V) is a compound of formula
(Va):
##STR00062## [0158] That is, the compound of formula (II) can be a
compound of formula (IIa):
##STR00063##
[0159] In some embodiments, the compound of formula (V) is a
compound of (Vb):
##STR00064## [0160] That is, the compound of formula (I) can be a
compound of formula (Ib):
##STR00065##
[0161] In some embodiments, the compound of formula (V) is a
compound of (Vc):
##STR00066##
[0162] Suitable protecting groups vary depending on the nature of
the reactions taking place, e.g., the suitable conditions for
producing a compound of formula (V). In some embodiments, suitable
oxygen protecting groups for R.sub.3 include acetate groups, ester
groups, benzyl groups and benzoate groups. In some embodiments,
suitable oxygen protecting groups for R.sub.4 include silyl groups.
In some embodiments, suitable oxygen protecting groups for R.sub.13
include silyl groups. In some embodiments, the protection of oxygen
by R.sub.13 results in a predominantly cis-olefin product, whereas
if R.sub.13 is a hydrogen, a predominantly trans olefin is formed.
In some embodiments, the cis olefin is preferred.
[0163] In some embodiments, the double bond represented by
##STR00067##
represents a double bond where the substituents are situated in a
cis position, relative to each other.
[0164] In some aspects, the present invention is directed to
methods for making a compound of formula (VI):
##STR00068## [0165] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0166] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0167] R.sub.4 is selected from the group consisting of hydrogen
and a base stable oxygen protecting group; [0168] R.sub.8 is
selected from the group consisting of hydrogen and --OR.sub.g
wherein R.sub.g is hydrogen or a base stable oxygen protecting
group, [0169] R.sub.9 is selected from the group consisting of
hydrogen, halogen, --OR.sub.b, C.sub.1-6 alkyl, C.sub.3-6
unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6
haloalkyl, --SR.sub.d and --NR.sub.eR.sub.f wherein R.sub.b is
hydrogen or a base stable oxygen protecting group, wherein R.sub.d
is selected from the group consisting of hydrogen, C.sub.1-6 alkyl,
C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7
membered heteroaryl comprising 1, 2 or 3 heteroatoms, and C.sub.5-7
aryl and wherein R.sub.e and R.sub.f are each independently
selected from the group consisting of hydrogen, C.sub.1-6 alkyl,
C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7
membered heteroaryl comprising 1, 2 or 3 heteroatoms, and C.sub.5-7
aryl or a base stable nitrogen protecting group; and [0170]
R.sub.10 is selected from the group consisting of hydrogen,
halogen, --OR.sub.c, C.sub.1-6 alkyl, C.sub.3-6 unconjugated
alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6 haloalkyl and
C.sub.1-6 alkoxy, wherein R.sub.c is hydrogen or a base stable
oxygen protecting group; and [0171] R.sub.11 and R.sub.12 are each
independently selected from the group consisting of hydrogen and a
base stable oxygen protecting group; or R.sub.11 and R.sub.12 are
taken together to form a 5 membered heterocyclyldiyl of structure
(a):
##STR00069##
[0171] wherein R.sub.19 and R.sub.20 are each independently
selected from the group consisting of hydrogen, C.sub.1-6 alkyl,
C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl, or R.sub.19 and
R.sub.20 together represent a fluorenyl moiety of structure
(b):
##STR00070##
[0172] In some embodiments, suitable conditions for forming a
compound of formula (VI) are suitable basic conditions. In some
embodiments, suitable conditions for forming a compound of formula
(V) include the use of a palladium catalyst. Palladium catalysts
can be used in organic chemistry to facilitate carbon-carbon bond
formation, e.g., by coordinating to a double bond of one fragment
to form a pi-coordinated complex. Exemplary palladium catalysts
include, but are not limited to
tetrakis(triphenylphosphine)palladium(0), palladium chloride and
palladium(II) acetate. In some embodiments, suitable conditions for
forming a compound of formula (V) include Heck coupling conditions.
In some embodiments suitable Heck coupling conditions include, but
are not limited to the reaction of an unsaturated halide or
triflate with an alkene and a strong base and palladium catalyst to
form a substituted alkene.
[0173] Compounds of formula (VI) are synthesized by reacting a
compound of formula (I):
##STR00071##
with a compound of formula (III):
##STR00072## [0174] wherein Y is a halogen or a triflate
(--O--SO.sub.2CF.sub.3) under suitable conditions, such that a
compound of formula (VI) is formed.
[0175] The compounds of formula (I) and formula (III) can have
varying stereochemistries at each chiral carbon. In preferred
embodiments, the compounds of formula (I) and formula (III) have a
specific stereochemistry at each chiral carbon. Accordingly, the
resulting compound of formula (VI) can have varying
stereochemistries at each chiral carbon. For example, in some
embodiments, the compound of formula (VI) is a compound of formula
(VIb):
##STR00073##
That is, the compound of formula (I) can be a compound of formula
(Ib):
##STR00074##
[0176] Suitable protecting groups vary depending on the nature of
the reactions taking place, e.g., the suitable conditions for
producing a compound of formula (VI). In some embodiments, suitable
oxygen protecting groups for R.sub.3 include acetate groups, ester
groups, benzyl groups and benzoate groups. In some embodiments,
suitable oxygen protecting groups for R.sub.4 include silyl groups.
Suitable protecting groups (oxygen and/or nitrogen) for the
substituents of R.sub.8, R.sub.9 and R.sub.10 (i.e.,
R.sub.b-R.sub.g) will be dependent upon the resulting substituent.
For example, under the suitable basic conditions of the reaction to
produce a compound of formula (VI) wherein R.sub.9 is
--NR.sub.eR.sub.f, R.sub.e and R.sub.f can each independently be a
suitably base stable nitrogen protecting group, e.g., BOC.
Similarly, under the suitable basic conditions of the reaction to
produce a compound of formula (VI) wherein R.sub.9 is --OR.sub.b,
R.sub.b can be a suitably base stable oxygen protecting group. In
some embodiments, suitable oxygen protecting groups for R.sub.13
include silyl groups.
[0177] In some embodiments, the double bond represented by
##STR00075##
represents a double bond where the substituents are situated in a
cis position, relative to each other.
[0178] In some aspects, the present invention is directed to
methods for making a compound of formula (IV):
##STR00076## [0179] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; R.sub.5 and R.sub.6 are each independently
selected from the group consisting of hydrogen, halogen, C.sub.1-6
alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl,
C.sub.1-6 alkoxy, phenyl and benzyl, wherein the phenyl or benzyl
are substituted with 0, 1, 2, or 3 substituents independently
selected from halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or
R.sub.5 and R.sub.6 are taken together with the carbons on which
they are attached to form a 5-6 membered unconjugated carbocyclic
ring; [0180] R.sub.7 is selected from the group consisting of
hydrogen and --OR.sub.a wherein R.sub.a is hydrogen or a base
stable oxygen protecting group; [0181] R.sub.8 is selected from the
group consisting of hydrogen and --OR.sub.g wherein R.sub.g is
hydrogen or a base stable oxygen protecting group; [0182] R.sub.9
is selected from the group consisting of hydrogen, halogen,
--OR.sub.b, C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl,
C.sub.3-6 unconjugated alkynyl, C.sub.1-6 haloalkyl, --SR.sub.d and
--NR.sub.eR.sub.f wherein R.sub.b is hydrogen or a base stable
oxygen protecting group, wherein R.sub.d is selected from the group
consisting of hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl,
C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl
comprising 1, 2 or 3 heteroatoms, and C.sub.5-7 aryl and wherein
R.sub.e and R.sub.f are each independently selected from the group
consisting of hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl,
C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl
comprising 1, 2 or 3 heteroatoms, and C.sub.5-7 aryl or a base
stable nitrogen protecting group; and [0183] R.sub.10 is selected
from the group consisting of hydrogen, halogen, --OR.sub.c,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl, C.sub.3-6
unconjugated alkynyl, C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy,
wherein R.sub.e is hydrogen or a base stable oxygen protecting
group; and [0184] R.sub.11 and R.sub.12 are each independently
selected from the group consisting of hydrogen and a base stable
oxygen protecting group; or R.sub.11 and R.sub.12 are taken
together to form a 5 membered heterocyclyldiyl of structure
(a):
[0184] ##STR00077## [0185] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
##STR00078##
[0186] Compounds of formula (IV) are synthesized by combining a
compound of formula (I) with a compound of formula (II) or a
compound of formula (III) under suitable conditions, such that a
compound of formula (IV) is formed. Compounds of formula (I),
formula (II) and formula (III) are those listed above, in relation
to the synthesis of compounds of formula (V) and formula (VI). In
some embodiments, compounds of formula (IV) are synthesized by
combining a compound of formula (I) with a compound of formula (II)
or a compound of formula (III) under suitable conditions, such that
an alpha-intermediate and a compound of formula (IV) are formed. In
some embodiments, the alpha-intermediate is a compound of formula
(V). In other embodiments, the alpha-intermediate is a compound of
formula (VI).
[0187] In some embodiments, the synthesis of compounds of formula
(IV) is not limited by inclusion of all three specific starting
compounds (i.e., compounds of formula (I), formula (II) and formula
(III)). That is, as long as compound (I) is reacted with either
compound (II) or compound (III), the remainder of the synthesis may
proceed via a route that does not specifically include the third
compound. Accordingly, in some embodiments, the compound of formula
(I) is reacted with the compound of formula (II) to form a compound
of formula (V), which is subsequently used in the synthesis of a
compound of formula (IV). In other embodiments, the compound of
formula (I) is reacted with the compound of formula (III) to form a
compound of formula (VI), which is subsequently used in the
synthesis of a compound of formula (IV).
[0188] In some embodiments, the synthesis of compounds of formula
(IV) is limited by inclusion of all three specific starting
compounds (i.e., compounds of formula (I), formula (II) and formula
(III)). That is, in some embodiments, the present invention
includes a method for making a compound of formula (IV) comprising
combining a compound of formula (I) with a compound of formula (II)
and a compound of formula (III), under suitable conditions, such
that a compound of formula (IV) is formed. Accordingly, in some
embodiments, the compound of formula (I) is reacted with the
compound of formula (II) to form a compound of formula (V), which
is subsequently reacted with the compound of formula (III). In
other embodiments, the compound of formula (I) is reacted with the
compound of formula (III) to form a compound of formula (VI), which
is subsequently reacted with the compound of formula (II).
[0189] Suitable protecting groups vary depending on the nature of
the reactions taking place, e.g., the suitable conditions for
producing a compound of formula (IV). In some embodiments, suitable
oxygen protecting groups for R.sub.3 include acetate groups, ester
groups, benzyl groups and benzoate groups. In some embodiments,
suitable oxygen protecting groups for R.sub.4 include silyl groups.
Again, suitable protecting groups (oxygen and/or nitrogen) for the
substituents of R.sub.8, R.sub.9 and R.sub.10 (i.e.,
R.sub.b-R.sub.g) will be dependent upon the resulting substituent.
For example, under the suitable basic conditions of the reaction to
produce a compound of formula (VI) wherein R.sub.9 is
--NR.sub.eR.sub.f, R.sub.e and R.sub.f can each independently be a
suitably base stable nitrogen protecting group, e.g., BOC.
Similarly, under the suitable basic conditions of the reaction to
produce a compound of formula (VI) wherein R.sub.9 is --OR.sub.b,
R.sub.b can be a suitably base stable oxygen protecting group. In
some embodiments, suitable oxygen protecting groups for R.sub.13
include silyl groups and/or ester groups or allylic groups.
[0190] As with the synthesis of compounds of formula (V) and
formula (VI), the compounds of formula (I), formula (II) and
formula (III) can have varying stereochemistries at each chiral
carbon. In preferred embodiments, the compounds of formula (I),
formula (II) and formula (III) have a specific stereochemistry at
each chiral carbon. Accordingly, the resulting compound of formula
(IV) can have varying stereochemistries at each chiral carbon. For
example, in some embodiments, the compound of formula (IV) is a
compound of formula (IVa):
##STR00079##
That is, the compound of formula (II) can be a compound of formula
(IIa), as described above.
[0191] The various substituents on each of formulae (I)-(VI) can be
present in any combination. In some embodiments, R.sub.1 is
hydrogen. In some embodiments, R.sub.2 is hydrogen. In some
embodiments, R.sub.1 and R.sub.2 are both hydrogen. In some
embodiments, R.sub.5 is hydrogen or a
C.sub.1-6 alkyl. In some embodiments, R.sub.6 is hydrogen or a
C.sub.1-6 alkyl. In some embodiments, R.sub.5 and R.sub.6 are each
independently hydrogen or methyl. In some embodiments, R.sub.5 and
R.sub.6 are taken together to form a 5-6 membered unconjugated
carbocyclic ring, e.g., cyclopentyl, unconjugated cyclopentenyl,
cyclohexyl or unconjugated cyclohexenyl. In some embodiments,
R.sub.7 is hydrogen or hydroxyl. In some embodiments, R.sub.8 is
hydrogen or hydroxyl. In some embodiments, R.sub.9 is --OR.sub.b or
--NR.sub.eR.sub.f. In some embodiments, R.sub.b is hydrogen or a
C.sub.1-6 alkyl. In some embodiments, R.sub.9 is --NR.sub.eR.sub.f.
In some embodiments, R.sub.e is hydrogen or a C.sub.1-6 alkyl. In
some embodiments, R.sub.f is hydrogen, a C.sub.1-6 alkyl or a base
stable nitrogen protecting group. In some embodiments, R.sub.e is
C.sub.1-6 alkyl, e.g., methyl or ethyl, and R.sub.f is hydrogen or
a base stable nitrogen protecting group. In some embodiments,
R.sub.10 is hydrogen.
[0192] The present invention is based, at least in part, on the
control of the stereochemistry at the carbon indicated below in
formula (V):
##STR00080##
and/or the carbon indicated below in formula (VI):
##STR00081##
In previous methods of forming the compounds of formula (IV), the
preparation of this fragment afforded a 1:2 mixture of the .alpha.
and .beta. isomers of formula (V). It was additionally determined
by the present inventors that the .alpha. and .beta. isomers
produced vastly different yields when used in the subsequent
synthesis of the final macrolide product. For example, in the
production of compound 010, where the .alpha. and .beta. isomers of
the compounds of formula (V) were:
##STR00082##
the yields obtained from subsequent reactions are shown below in
Table 1.
TABLE-US-00001 TABLE 1 Yields of reaction steps in the production
of compound 010 Stage .alpha. isomer yields.dagger. .beta. isomer
yields.dagger-dbl. Heck Coupling 75% 60-65% Ethylation 82% ND* TBS
Cleavage 75% 42%** Macrolactonization 76% 20% *** Phenol Protection
99% ND MPM Removal 98% 86% PCC Oxidation 81% 82% *The ethylation
reaction generated an ~1:1 mixture of the desired product and an
elimination product. The 2 materials were not separable. **Yield of
the TBS cleavage reaction is based upon the 1:1 mixture mentioned
above. *** The yield of the macrolactonization reaction can be
improved to 63% by changing to lithium
bis(trimethylsilyl)amide.
[0193] As can be seen in Table 1, the .beta. isomer of the compound
of formula (V) afforded significantly less yield in a number of the
subsequent reactions steps. Moreover, the elimination byproduct
which occurred in the ethylation of the .beta. isomer is indicative
of not only a significant loss in yield, but also to an impurity
which is difficult to separate. Accordingly, and without wishing to
be bound by any particular theory, it is believed that the
elimination of the .beta. isomer of the compound of formula (V)
would produce not only a higher yield in subsequent reactions, but
also a purer final product.
[0194] In some embodiments, the compounds and compositions are
substantially free of elimination products, e.g. compounds of
formula (XI):
##STR00083##
In some embodiments, the compounds and compositions are
substantially free the elimination product represented by the
following structure:
##STR00084##
[0195] Accordingly, in some aspects, the present invention is
directed to method for making an alpha-enhanced composition
comprising a compound of formula (V). It was determined by the
present inventors that an alpha-enhanced composition can be
obtained by using starting materials having the appropriate
stereochemistry. Accordingly, the alpha enhanced composition
comprising a compound of formula (V) can be formed by reacting a
compound of formula (I) with a compound of formula (II) under
suitable conditions, such that an alpha-enhanced composition
comprising a compound of formula (V) is formed. Similarly, in some
aspects, the present invention is directed to method for making an
alpha-enhanced composition comprising a compound of formula (VI).
Such an alpha enhanced composition can be formed by reacting a
compound of formula (I) with a compound of formula (III) under
suitable conditions, such that an alpha-enhanced composition
comprising a compound of formula (VI) is formed. In some
embodiments, the compound of formula (I) is a compound of formula
(Ib):
##STR00085##
[0196] The present invention is also based, at least in part, on
the fact that crystalline intermediates can be isolated, such that
minimal chromatography is needed in the synthesis of the macrolide
product. In previous methods of forming compounds of formula (IV),
numerous chromatographic purifications were needed to remove
impurities. (See, e.g., WO 03/076424, paragraphs [1099]-[1119])
Accordingly, and without wishing to be bound by any particular
theory, it is believed that the elimination of chromatographic
steps with maintenance of the appropriate purity and
stereochemistry would improve the yield of the final macrolide
product.
[0197] Accordingly, in some embodiments, the compound of formula
(I) is crystalline. In other embodiments, the compound of formula
(II) is crystalline. In still other embodiments, the compound of
formula (III) is crystalline. In other embodiments, the compound of
formula (IV) is produced in substantially pure form without the use
of chromatography. In other embodiments, the compound of formula
(V) is produced in substantially pure form without the use of
chromatography. In other embodiments, the compound of formula (VI)
is produced in substantially pure form without the use of
chromatography.
[0198] In still other embodiments, the compound of formula (V)
and/or the compound of formula (VI) is crystalline. It will be
appreciated by the skilled artisan that the crystallization of
intermediates does not necessarily proceed effortlessly or
efficiently. Accordingly, in some embodiments, a crystallizable
analog of the compound formula (V) and/or the compound of formula
(VI) is formed as an intermediate. As used herein, the term
"crystallizable analogs" refers to compounds of formula (V) and/or
formula (VI) which have been modified such that they are able to be
crystallized, while still maintaining their reactivity in
subsequent reaction steps. For example, compounds of formula (V)
and/or formula (VI) can be modified at one of the pendent oxygens
with a protecting group, such that the protecting group facilitates
crystallization. When the crystallizable analog is employed in
subsequent steps, the protecting group can then be removed.
[0199] For example, without wishing to be bound by any particular
theory, it is believed that introducing two conjugated rings into
one or more of the molecules of the present invention can
facilitate crystallization, e.g., by creating a favorable
interaction between the two rings. Such interaction can be, for
example, stacking of the rings due to the interaction of the
pi-orbitals. This phenomenon may be referred to as "pi-stacking."
Thus, in some embodiments, the molecules of the invention comprise
two conjugated rings, which are capable of pi-stacking. In some
embodiments, the rings comprise one or more substituents that
facilitate pi-stacking. For example, pi-stacking may be enhanced by
providing rings with dissimilar electronic characteristics (e.g.,
one electron-rich ring and one electron-poor ring). Such rings may
be chosen, for example, based upon the presence of certain electron
donating groups and/or electron withdrawing groups. That is, the
presence of electron donating groups will typically render a ring
more electron-rich, whereas the presence of electron withdrawing
groups will typically render a ring more electron-poor. Exemplary
electron donating groups include, but are not limited to --O.sup.-,
--OH, --OR, --NH.sub.2, --NR.sub.2, amides, --OCOR, alkyls (e.g.,
branched alkyls), phenyl groups and conjugated alkenyls. Exemplary
electron withdrawing groups include, but are not limited to,
--NO.sub.2, --NH.sub.3.sup.+, --NR.sub.3.sup.+, --SO.sub.3H,
nitrile, --CF.sub.3, carbonyl groups (e.g., --COH, --COR, --COOH
and --COOR) and halogens.
[0200] In some embodiments, the crystallizable analog of the
compound of formula (V) is a compound of formula (VII):
##STR00086##
e.g., formula (VII'):
##STR00087## [0201] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0202] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0203] R.sub.5 and R.sub.6 are each independently selected from the
group consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
[0204] R.sub.11 and R.sub.12 are each independently selected from
the group consisting of hydrogen and a base stable oxygen
protecting group; or R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
[0204] ##STR00088## [0205] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
[0205] ##STR00089## [0206] R.sub.13 is a moiety of formula
(VIII):
##STR00090##
[0206] and [0207] R.sub.14, R.sub.15, R.sub.16, R.sub.17 and
R.sub.18 are each independently selected from the group consisting
of H, NO.sub.2, --NH.sub.3.sup.+, --COH, --CO(C.sub.1-4 alkyl),
--COCl, --COOH, --COO(C.sub.1-4 alkyl), --NR.sub.3.sup.+,
--SO.sub.3H, nitrile, --CF.sub.3 and halogen.
[0208] In some embodiments, R.sub.3 is a first aromatic ring
containing oxygen protecting group, e.g., a benzyl or benzoyl
substituted with 0, 1, 2 or 3 substituents independently selected
from --OH, --O(C.sub.1-4alkyl), --NH.sub.2, --NH(C.sub.1-4alkyl),
--N(C.sub.1-4alkyl).sub.2, amides, --OCO(C.sub.1-4alkyl) and
(C.sub.1-4alkyl).
[0209] In some embodiments, the crystallizable analog of the
compound of formula (V) is a compound of formula (VII):
##STR00091##
e.g., formula (VII'):
##STR00092## [0210] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0211] R.sub.3 is a first aromatic
ring-containing oxygen protecting group; [0212] R.sub.5 and R.sub.6
are each independently selected from the group consisting of
hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6
alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl,
wherein the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; [0213] R.sub.11 and
R.sub.12 are each independently selected from the group consisting
of hydrogen and a base stable oxygen protecting group; or R.sub.11
and R.sub.12 are taken together to form a 5 membered
heterocyclyldiyl of structure (a):
[0213] ##STR00093## [0214] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
[0214] ##STR00094## [0215] R.sub.13 is a second aromatic
ring-containing oxygen protecting group.
[0216] In some embodiments, R.sub.13 is a benzoyl or benzyl
substituted with at least one substituent, where each substituent
is independently selected from --NO.sub.2, --NH.sub.3.sup.+,
--NH.sub.2(C.sub.1-4alkyl).sup.+, --NH(C.sub.1-4alkyl).sub.2.sup.+,
--N(C.sub.1-4alkyl).sub.3.sup.+, --SO.sub.3H, nitrile, --CF.sub.3,
--COH, --CO(C.sub.1-4alkyl), --COOH and --COO(C.sub.1-4alkyl).
[0217] In some embodiments, R.sub.13 is a moiety of formula
(VIII):
##STR00095##
[0218] As used herein, the term "aromatic ring containing oxygen
protecting group" refers to an oxygen protecting group as described
in more detail herein, which possesses in the structure at least
one aromatic ring. An aromatic ring refers to a ring system (e.g.,
benzene) containing conjugated double bonds. Such a structure
typically results in electrons delocalized around the ring system.
In some embodiments, the aromatic ring-containing oxygen protecting
group is a substituted or unsubstituted benzoyl. In some
embodiments, the aromatic ring-containing oxygen protecting group
is a substituted or unsubstituted benzyl.
[0219] In some embodiments, the first aromatic ring-containing
oxygen protecting group is a benzoyl or benzyl substituted with at
least one electron withdrawing group and the second aromatic
ring-containing oxygen protecting group is a benzoyl or benzyl
substituted with at least one electron donating group. In other
embodiments, the first aromatic ring-containing oxygen protecting
group is a benzoyl or benzyl substituted with at least one electron
donating group and the second aromatic ring-containing oxygen
protecting group is a benzoyl or benzyl substituted with at least
one electron withdrawing group. The electron donating and electron
withdrawing groups may be any of those described above. In some
embodiments, the first aromatic ring-containing oxygen protecting
group is a benzoyl or benzyl substituted with at least one
substituent, where each substituent is independently selected from
--OH, --O(C.sub.1-4alkyl), --NH.sub.2, --NH(C.sub.1-4alkyl),
--N(C.sub.1-4alkyl).sub.2, amides, --OCO(C.sub.1-4alkyl) and
(C.sub.1-4alkyl). In some embodiments, the second aromatic
ring-containing oxygen protecting group is a benzoyl or benzyl
substituted with at least one substituent, where each substituent
is independently selected from --NO.sub.2, --NH.sub.3.sup.+,
--NH.sub.2(C.sub.1-4alkyl).sup.+, --NH(C.sub.1-4alkyl).sub.2.sup.+,
--N(C.sub.1-4alkyl).sub.3.sup.+, --SO.sub.3H, nitrile, --CF.sub.3,
--COH, --CO(C.sub.1-4alkyl), --COOH and --COO(C.sub.1-4alkyl).
[0220] Regarding compounds of formula (VII), in some embodiments,
R.sub.1 is hydrogen. In some embodiments, R.sub.2 is hydrogen. In
some embodiments, R.sub.1 and R.sub.2 are both hydrogen. In some
embodiments, R.sub.3 is a benzyl substituted with 0, 1, 2, or 3
substituents independently selected from --OH, --O(C.sub.1-4alkyl),
--NH.sub.2, --NH(C.sub.1-4alkyl), --N(C.sub.1-4alkyl).sub.2,
amides, --OCO(C.sub.1-4alkyl) and (C.sub.1-4alkyl). In some
embodiments, R.sub.3 is 4-methoxybenzyl. In some embodiments,
R.sub.5 is hydrogen or a C.sub.1-6 alkyl. In some embodiments,
R.sub.6 is hydrogen or a C.sub.1-6 alkyl. In some embodiments,
R.sub.5 and R.sub.6 are each independently hydrogen or methyl.
[0221] In some embodiments, one of R.sub.14, R.sub.15, R.sub.16,
R.sub.17, and R.sub.18 is selected from the group consisting of
NO.sub.2, --NH.sub.3.sup.+, --COH, --CO(C.sub.1-4 alkyl), --COCl,
--COOH, --COO(C.sub.1-4 alkyl), --NR.sub.3.sup.+, --SO.sub.3H,
nitrile, --CF.sub.3 and halogen and the other four of R.sub.14,
R.sub.15, R.sub.16, R.sub.17, and R.sub.18 are hydrogen. In some
embodiments, at least one of R.sub.14, R.sub.15, R.sub.16,
R.sub.17, and R.sub.18 is selected from the group consisting of
NO.sub.2, --NH.sub.3.sup.+, --COH, --CO(C.sub.1-4 alkyl), --COCl,
--COOH, --COO(C.sub.1-4 alkyl), --NR.sub.3.sup.+, --SO.sub.3H,
nitrile, --CF.sub.3 and halogen and the other of R.sub.14,
R.sub.15, R.sub.16, R.sub.17, and R.sub.18 are hydrogen. In some
embodiments, R.sub.16 is NO.sub.2 and each of R.sub.14, R.sub.15,
R.sub.17, and R.sub.18 are independently hydrogen.
[0222] In some embodiments, the double bond represented by
##STR00096##
represents a double bond where the substituents are situated in a
cis position, relative to each other.
[0223] In some embodiments, the compound of formula (VII) is a
compound of formula (VIIa)
##STR00097##
[0224] In some embodiments, the compound of formula (VII) is a
compound of formula (VIIb):
##STR00098##
[0225] The present invention is also based, at least in part, on
the fact that highly soluble intermediates can be separated from
insoluble or sparingly soluble impurities, such that less
chromatography is needed in the synthesis of the macrolide product.
As indicated supra, previous methods of forming compounds of
formula (IV) relied on numerous chromatographic purifications to
remove impurities. Again, without wishing to be bound by any
particular theory, it is believed that the elimination of one or
more of such chromatographic steps with maintenance of the
appropriate purity and stereochemistry would not only improve the
yield of the final macrolide product, but also lower the time and
cost of production and decrease the quantity of reagents, e.g.,
organic solvents, utilized in the synthesis of compounds of formula
(IV).
[0226] Accordingly, in some embodiments, the intermediates of the
present invention can be rendered highly soluble by the attachment
of a solubility-promoting group. In some embodiments, the
attachment of a solubility-promoting group is reversible. For
example, in some embodiments the addition of the
solubility-promoting group may be likened to the addition of a
nitrogen or oxygen protecting group. Without wishing to be bound by
any particular theory, it is believed that the attachment of a
solubility-promoting group allows the target compound to be drawn
into water, thus leaving any moiety that did not become derivatized
with the solubility-promoting group in the organic media.
Accordingly, in some embodiments, a soluble analog of the compound
formula (I) is formed as an intermediate. As used herein, the term
"soluble analogs" refers to compounds of formula (I) which have
been modified (e.g., by the attachment of a solubility-promoting
group) such that they are soluble in aqueous solutions. For
example, compounds of formula (I) and/or formula (VI) can be
modified at one of the pendent oxygens with a solubility-promoting
group, such that the solubility-promoting group facilitates
dissolution of the compound in aqueous medium. The skilled artisan
may then take advantage of the difference in solubility of the
compound of interest and the impurities to isolate the compound of
interest without the use of chromatography. The
solubility-promoting group can be removed subsequent to isolation
of the compound If interest.
[0227] In some embodiments, the soluble analog of the compound of
formula (I) is a compound of formula (XIII):
##STR00099##
e.g., formula (XIII'):
##STR00100## [0228] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0229] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0230] R.sub.11 and R.sub.12 are each independently selected from
the group consisting of hydrogen and a base stable oxygen
protecting group; or R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
[0230] ##STR00101## [0231] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
##STR00102##
[0231] and [0232] one, two or three of R.sub.21, R.sub.22,
R.sub.23, R.sub.24, and R.sub.25 are acidic-hydrogen containing
moieties or salts thereof, and the remainder of R.sub.21, R.sub.22,
R.sub.23, R.sub.24, and R.sub.25 are each independently hydrogen,
methyl, hydroxyl or amino
[0233] As used herein, the term "acidic-hydrogen containing moiety"
refers to a substituent group that includes at least one acidic
hydrogen. Acidic-hydrogen containing moieties include, for example,
--COOH, --SO.sub.3H, --SO.sub.4H, --PO.sub.3H.sub.2 and
--PO.sub.4H.sub.2. It is to be understood that an acidic-hydrogen
containing moiety may be positioned ortho, meta or para to the core
of the compound of formula (XII), i.e., the
##STR00103##
moiety. In some embodiments, an acidic-hydrogen containing moiety
is ortho to the core of the compound of formula (XII).
[0234] In some embodiments, R.sub.21 is --COOH or a salt thereof.
In some embodiments R.sub.21 is --SO.sub.3H or a salt thereof. In
some embodiments, R.sub.22, R.sub.23, R.sub.24, and R.sub.25 are
each independently hydrogen. In some embodiments, R.sub.22 is
--COOH or a salt thereof. In some embodiments R.sub.22 is
--SO.sub.3H or a salt thereof. In some embodiments, R.sub.21,
R.sub.23, R.sub.24, and R.sub.25 are each independently
hydrogen.
[0235] In some embodiments, R.sub.1, R.sub.2, R.sub.3, R.sub.11,
R.sub.12, R.sub.19 and R.sub.20 are selected from the substituents
provided in connection with Compound (I). For example, in some
embodiments, R.sub.1 and R.sub.2 are each independently hydrogen.
In some embodiments, R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
##STR00104##
wherein R.sub.19 and R.sub.20 are each independently C.sub.1-6
alkyl.
[0236] In some embodiments, the compounds of formula (I), formula
(II) and formula (III) are reacted to form a compound of formula
(IV'):
##STR00105##
which is subsequently converted to a compound of formula (IV),
e.g., by macrolactonization using potassium t-butoxide and
subsequent deprotection/oxidation. It has also been determined that
the removal of a protecting group at the R.sub.3 position of
formula (IV') (e.g., the DDQ removal of the p-methoxybenzyl ether
to form a hydroxy moiety) generates p-anisaldehyde. p-Anisaldehyde
has been found, in turn, to be responsible for the formation of a
dimeric impurity:
##STR00106##
wherein substituents are as described above. Although the dimer was
re-subjected to the reaction conditions, it did not degrade back to
the monomer and p-anisaldehyde. This indicates that it is stable.
Also, some initial biological testing indicated that the dimer had
lower potency and greater cytotoxicity than the monomer, making it
an undesirable impurity.
[0237] Accordingly, in some embodiments, the compounds and
compositions of the present invention are substantially free of
dimeric products. In some embodiments, a semicarbazide
hydrochloride in the presence of sodium acetate is added to the
reaction mixture. Without wishing to be bound by any particular
theory, it is believed that this may form an imine derivative,
which could precipitate from solution and be removed by
filtration.
[0238] In some embodiments, the compounds of formula (I), formula
(II) and formula (III) are reacted to form a compound of formula
(IV''):
##STR00107## [0239] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0240] R.sub.3 is a base stable oxygen
protecting group; [0241] R.sub.5 and R.sub.6 are each independently
selected from the group consisting of hydrogen, halogen, C.sub.1-6
alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl,
C.sub.1-6 alkoxy, phenyl and benzyl, wherein the phenyl or benzyl
are substituted with 0, 1, 2, or 3 substituents independently
selected from halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or
R.sub.5 and R.sub.6 are taken together with the carbons on which
they are attached to form a 5-6 membered unconjugated carbocyclic
ring; [0242] R.sub.7 is --OR.sub.a wherein R.sub.a is a base stable
oxygen protecting group; [0243] R.sub.8 is selected from the group
consisting of hydrogen and --OR.sub.g wherein R.sub.g is hydrogen
or a base stable oxygen protecting group; [0244] R.sub.9 is
selected from the group consisting of hydrogen, halogen,
--OR.sub.b, C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl,
C.sub.3-6 unconjugated alkynyl, C.sub.1-6 haloalkyl, --SR.sub.d and
--NR.sub.eR.sub.f wherein R.sub.b is hydrogen or a base stable
oxygen protecting group, wherein R.sub.d is selected from the group
consisting of hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl,
C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl
comprising 1, 2 or 3 heteroatoms, and C.sub.5-7 aryl and wherein
R.sub.e and R.sub.f are each independently selected from the group
consisting of hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl,
C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl
comprising 1, 2 or 3 heteroatoms, and C.sub.5-7 aryl or a base
stable nitrogen protecting group; and [0245] R.sub.10 is selected
from the group consisting of hydrogen, halogen, --OR.sub.c,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl, C.sub.3-6
unconjugated alkynyl, C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy,
wherein R.sub.e is hydrogen or a base stable oxygen protecting
group; which is subsequently converted to a compound of formula
(IV), e.g., by subsequent deprotection/oxidation. Deprotection can
occur at any or all of R.sub.3, R.sub.7 or the structure
represented by the heterocyclyldiyl of formula XII:
##STR00108##
[0245] In some embodiments, deprotection occurs at all of R.sub.3,
R.sub.7 and the structure represented by the heterocyclyldiyl of
formula (XII) at the same time. In other embodiments, deprotection
occurs at R.sub.7 first, followed by subsequent deprotection at
R.sub.3 and the structure represented by the heterocyclyldiyl of
formula (XII).
[0246] In other aspects, the present invention is directed to
methods for making a purity-enhanced composition comprising a
compound of formula (IV). It was also determined by the present
inventors that a purity-enhanced composition can be obtained by
using starting materials having the appropriate stereochemistry,
and thus forming alpha-enhanced intermediates (e.g., alpha-enhanced
compositions comprising formula (V) or formula (VI)) as well as
targeting crystalline intermediates which can be isolated in
substantially pure form without chromatography. The methods for
making a purity-enhanced composition comprising a compound of
formula (IV) typically include combining a compound of formula (I)
with a compound of formula (II) and a compound of formula (III),
under suitable conditions, such that a purity-enhanced composition
comprising a compound of formula (IV) is formed.
[0247] Similarly, in other aspects, the present invention is
directed to methods for making a composition comprising a compound
of formula (IV), wherein the composition is substantially free of
organic impurities. The methods typically include combining a
compound of formula (I) with a compound of formula (II) and a
compound of formula (III), under suitable conditions, such that a
composition comprising a compound of formula (IV) substantially
free of organic impurities is formed.
[0248] In still other aspects, the present invention is directed to
methods for making a yield-enhanced composition comprising a
compound of formula (IV). The methods typically include combining a
compound of formula (I) with a compound of formula (II) and a
compound of formula (III), under suitable conditions, such that a
composition comprising a compound of formula (IV) is formed.
[0249] The skilled artisan would be able to appreciate that the
reaction conditions employed herein may vary. For example, many
reagents can be utilized in coupling the compounds of any of
formulae (I)-(III) and (V)-(VI) with one another. Moreover, many
reagents can be utilized in protection, deprotection,
macrolactonization and oxidation of various intermediates.
Additionally, the time taken for the reaction may vary depending
upon reagents and concentrations. Additionally, the reactions of
the present invention may occur at varying temperatures. Numerous
solvents can also be employed in the reactions of the present
invention. Suitable solvents are liquids at ambient room
temperature and pressure or remain in the liquid state under the
temperature and pressure conditions used in the reaction. Useful
solvents are not particularly restricted provided that they do not
interfere with the reaction itself (that is, they preferably are
inert solvents), and they dissolve a certain amount of the
reactants. Depending on the circumstances, solvents may be
distilled or degassed. Solvents may be, for example, aliphatic
hydrocarbons (e.g., hexanes, heptanes, ligroin, petroleum ether,
cyclohexane, or methylcyclohexane) and halogenated hydrocarbons
(e.g., methylenechloride, chloroform, carbontetrachloride,
dichloroethane, chlorobenzene, or dichlorobenzene); aromatic
hydrocarbons (e.g., benzene, toluene, tetrahydronaphthalene,
ethylbenzene, or xylene); ethers (e.g., diglyme, methyl-tert-butyl
ether, methyl-tert-amyl ether, ethyl-tert-butyl ether,
diethylether, diisopropylether, tetrahydrofuran or
methyltetrahydrofurans, dioxane, dimethoxyethane, or
diethyleneglycol dimethylether); nitriles (e.g., acetonitrile);
ketones (e.g., acetone); esters (e.g., methyl acetate or ethyl
acetate); alcohols (e.g., methanol) and mixtures thereof. The
skilled artisan would be able to determine, without undue
experimentation, proper reaction conditions using the teachings of
the present invention.
Analysis of Beneficial Reaction Properties
[0250] In one embodiment, the methods of preparation of the
invention are advantageous over the methods that currently in use
for the synthesis of macrolides of the present invention. In
certain embodiments, a method of the invention possesses a
beneficial reaction property (BRP).
[0251] The language "beneficial reaction property or BRP" includes
a property of one reaction that is beneficial over an existing
manner of performing the same reaction. The property may be any
property suitable to comparison to the existing methodology, such
that the property is equal to or better in nature than the property
of the existing methodology. Examples of such properties include,
without limitation, starting material safety, reaction time, energy
cost, reaction safety, product mass balance (reduction of waste),
reaction cleanliness, waste, throughput, workup, overall process
time, and overall cost of the target product. Several particular
examples of beneficial reaction properties as applied to the
preparation of compound 010 are discussed below.
Cost Effectiveness
[0252] In preparing compound 010, the ethylation of the aromatic
nitrogen (i.e., R.sub.9 in formula (IV)) is a throughput-limiting
step. In the methodology that has previously been used, the
ethylation occurs late in the synthesis where the entire carbon
skeleton is in place. Thus, any limitations of the ethylation
reaction place the entire skeleton, including all starting
materials, at risk. By performing the ethylation earlier, the risk
to other fragments is eliminated and overall synthetic convergence
is increased. Accordingly, in some embodiments, the substituent
R.sub.9 of the compound of formula (III) is such that no alkylation
needs to occur in subsequent steps in the formation of a compound
of formula (IV).
Waste/Impurities
[0253] Theoretically, for the previous methods for synthesizing
compound 010, at least 33% of the mass on the product side is
waste. For example, about 66% of the compound of formula (V)
produced in previous methods is the .beta. isomer, and about 50% of
the product resulting from the .beta. isomer is an inseparable
elimination by-product of the structure:
##STR00109##
[0254] Moreover, with regard to the total amount of waste, the
methods of the present invention can further reduce waste over
previous methods by the elimination of chromatography steps. Such
reduction of waste can lead, e.g., to further savings in time,
cost, hazardous waste treatments, etc.
Intermediates of the Invention
[0255] The present invention is also directed, at least in part, to
intermediates for use in the synthesis of compounds and
compositions of the present invention, e.g., compounds of formula
(IV). As discussed above, it is desirable to have crystalline
intermediates in the synthesis of a final product, at least so that
chromatography steps may be eliminated.
[0256] It is to be understood that, in addition to the specific
intermediates listed in Schemes 1 and 2, above, the present
invention also encompasses crystallizable analogs of such
intermediates. In some embodiments, one or more of the specific
intermediates listed in Schemes 1 and 2 will not readily or
efficiently crystallize. As described in more detail above,
intermediates can be modified such that they are able to be
crystallized. Such modified intermediates will maintain their
reactivity in subsequent reaction steps.
[0257] Accordingly, in some aspects the present invention is
directed to intermediates of formula (VII):
##STR00110## [0258] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0259] R.sub.3 is selected from the group
consisting of hydrogen and a base stable oxygen protecting group;
[0260] R.sub.5 and R.sub.6 are each independently selected from the
group consisting of hydrogen, halogen, C.sub.1-6 alkyl, C.sub.2-6
alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy,
phenyl and benzyl, wherein the phenyl or benzyl are substituted
with 0, 1, 2, or 3 substituents independently selected from
halogen, hydroxyl, C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and
R.sub.6 are taken together with the carbons on which they are
attached to form a 5-6 membered unconjugated carbocyclic ring;
[0261] R.sub.11 and R.sub.12 are each independently selected from
the group consisting of hydrogen and a base stable oxygen
protecting group; or R.sub.11 and R.sub.12 are taken together to
form a 5 membered heterocyclyldiyl of structure (a):
[0261] ##STR00111## [0262] R.sub.13 is a moiety of formula
(VIII):
##STR00112##
[0262] and [0263] R.sub.14, R.sub.15, R.sub.16, R.sub.17 and
R.sub.18 are each independently selected from the group consisting
of H, NO.sub.2, --NH.sub.3.sup.+, --COH, --CO(C.sub.1-4 alkyl),
--COCl, --COOH, --COO(C.sub.1-4 alkyl), --NR.sub.3.sup.+,
--SO.sub.3H, nitrile, --CF.sub.3 and halogen.
[0264] In certain aspects, the present invention is directed to
intermediates of formula (VII):
##STR00113## [0265] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0266] R.sub.3 is an aromatic ring-containing
oxygen protecting group; [0267] R.sub.5 and R.sub.6 are each
independently selected from the group consisting of hydrogen,
halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl, wherein
the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; [0268] R.sub.11 and
R.sub.12 are each independently selected from the group consisting
of hydrogen and a base stable oxygen protecting group; or R.sub.11
and R.sub.12 are taken together to form a 5 membered
heterocyclyldiyl of structure (a):
[0268] ##STR00114## [0269] wherein R.sub.19 and R.sub.20 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy and phenyl,
or R.sub.19 and R.sub.20 together represent a fluorenyl moiety of
structure (b):
[0269] ##STR00115## [0270] R.sub.13 is a an aromatic
ring-containing oxygen protecting group.
[0271] Exemplary values for R.sub.1, R.sub.2, R.sub.3, R.sub.5,
R.sub.6, R.sub.11, R.sub.12 and R.sub.13 are described in more
detail above in connection with the crystallizable analogs of
formula (VII).
[0272] In some embodiments, the compound of formula (VII) is a
compound of formula (VIIa)
##STR00116##
[0273] In some aspects, the present invention is directed to an
intermediate of formula (IX):
##STR00117## [0274] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl. In some embodiments, R.sub.1 is hydrogen. In
some embodiments, R.sub.2 is hydrogen. In some embodiments, R.sub.1
and R.sub.2 are each independently hydrogen.
[0275] In still other aspects the present invention is directed to
an intermediate of formula (X):
##STR00118## [0276] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0277] R.sub.5 and R.sub.6 are each
independently selected from the group consisting of hydrogen,
halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl, wherein
the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; [0278] R.sub.8 is selected
from the group consisting of hydrogen and --OR.sub.g wherein
R.sub.g is hydrogen or a base stable oxygen protecting group,
[0279] R.sub.9 is selected from the group consisting of hydrogen,
halogen, --OR.sub.b, C.sub.1-6 alkyl, C.sub.3-6 unconjugated
alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6 haloalkyl,
--SR.sub.d and --NR.sub.eR.sub.f wherein R.sub.b is hydrogen or a
base stable oxygen protecting group, wherein [0280] R.sub.d is
selected from the group consisting of hydrogen, C.sub.1-6 alkyl,
C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7
membered heteroaryl comprising 1, 2 or 3 heteroatoms, and C.sub.5-7
aryl and wherein R.sub.e and R.sub.f are each independently
selected from the group consisting of hydrogen, C.sub.1-6 alkyl,
C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, 5-7
membered heteroaryl comprising 1, 2 or 3 heteroatoms, and C.sub.5-7
aryl or a base stable nitrogen protecting group; and [0281]
R.sub.10 is selected from the group consisting of hydrogen,
halogen, --OR.sub.c, C.sub.1-6 alkyl, C.sub.3-6 unconjugated
alkenyl, C.sub.3-6 unconjugated alkynyl, C.sub.1-6 haloalkyl and
C.sub.1-6 alkoxy, wherein R.sub.e is hydrogen or a base stable
oxygen protecting group.
[0282] In some embodiments, R.sub.1 is hydrogen. In some
embodiments, R.sub.2 is hydrogen. In some embodiments, R.sub.1 and
R.sub.2 are each independently hydrogen. In some embodiments,
R.sub.5 is hydrogen or a C.sub.1-6 alkyl. In some embodiments,
R.sub.6 is hydrogen or a C.sub.1-6 alkyl. In some embodiments,
R.sub.5 and R.sub.6 are each independently hydrogen or methyl. In
some embodiments, R.sub.8 is hydrogen. In some embodiments,
R.sub.10 is hydrogen. In some embodiments, R.sub.9 is --OR.sub.b or
--NR.sub.eR.sub.f. In some embodiments, R.sub.b is hydrogen or a
C.sub.1-6 alkyl. In some embodiments, R.sub.9 is --NR.sub.eR.sub.f.
In some embodiments, R.sub.e is hydrogen or a C.sub.1-6 alkyl. In
some embodiments, R.sub.f is hydrogen, a C.sub.1-6 alkyl or a base
stable nitrogen protecting group. In some embodiments, R.sub.e is
C.sub.1-6 alkyl, e.g., methyl or ethyl, and R.sub.f is hydrogen or
a base stable nitrogen protecting group.
[0283] In some embodiments, the compound of formula (X) is a
compound of formula (Xa)
##STR00119## [0284] wherein R.sub.e is selected from the group
consisting of hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, [0285]
C.sub.2-6 alkynyl, C.sub.1-6 heteroalkyl, heteroaryl, and aryl; and
[0286] R.sub.f is a base stable nitrogen protecting group.
[0287] In other embodiments, the present invention is directed to
compositions that include intermediates of formula (X), wherein the
composition is substantially free of compounds of formula (IV):
##STR00120## [0288] wherein R.sub.1 and R.sub.2 are each
independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.3-6 unconjugated alkenyl and C.sub.3-6
unconjugated alkynyl; [0289] R.sub.5 and R.sub.6 are each
independently selected from the group consisting of hydrogen,
halogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, phenyl and benzyl, wherein
the phenyl or benzyl are substituted with 0, 1, 2, or 3
substituents independently selected from halogen, hydroxyl,
C.sub.1-3 alkyl, and NH.sub.2; or R.sub.5 and R.sub.6 are taken
together with the carbons on which they are attached to form a 5-6
membered unconjugated carbocyclic ring; [0290] R.sub.7 is
--OR.sub.a wherein R.sub.a is a base stable oxygen protecting
group; [0291] R.sub.8 is selected from the group consisting of
hydrogen and --OR.sub.g wherein R.sub.g is hydrogen or a base
stable oxygen protecting group; [0292] R.sub.9 is selected from the
group consisting of hydrogen, halogen, --OR.sub.b, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl, --SR.sub.d and [0293] --NR.sub.eR.sub.f
wherein R.sub.b is hydrogen or a base stable oxygen protecting
group, wherein R.sub.d is selected from the group consisting of
hydrogen, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl and wherein R.sub.e and R.sub.f are
each independently selected from the group consisting of hydrogen,
C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6
heteroalkyl, 5-7 membered heteroaryl comprising 1, 2 or 3
heteroatoms, and C.sub.5-7 aryl or a base stable nitrogen
protecting group; [0294] R.sub.10 is selected from the group
consisting of hydrogen, halogen, --OR.sub.c, C.sub.1-6 alkyl,
C.sub.3-6 unconjugated alkenyl, C.sub.3-6 unconjugated alkynyl,
C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.c is
hydrogen or a base stable oxygen protecting group; and R.sub.11 and
R.sub.12 are each independently hydrogen.
[0295] In other aspects the present invention is directed to an
intermediate of formula (III):
##STR00121## [0296] wherein Y is a halogen or a triflate
(--O--SO.sub.2CF.sub.3); [0297] R.sub.8 is selected from the group
consisting of hydrogen and --OR.sub.g wherein R.sub.g is hydrogen
or a base stable oxygen protecting group, [0298] R.sub.9 is
--NR.sub.eR.sub.f; wherein R.sub.e is selected from the group
consisting of C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6
alkynyl, C.sub.1-6 heteroalkyl, 5-7 membered heteroaryl comprising
1, 2 or 3 heteroatoms, and C.sub.5-7 aryl, and wherein R.sub.f is a
base stable nitrogen protecting group; and [0299] R.sub.10 is
selected from the group consisting of hydrogen, halogen,
--OR.sub.c, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl,
C.sub.1-6 haloalkyl and C.sub.1-6 alkoxy, wherein R.sub.e is
hydrogen or a base stable oxygen protecting group. In some
embodiments, R.sub.8 is hydrogen. In some embodiments, R.sub.10 is
hydrogen. In some embodiments, R.sub.e is a C.sub.1-6 alkyl, e.g.,
methyl or ethyl. In some embodiments, R.sub.f is --BOC.
[0300] In still other aspects, the present invention is directed to
an intermediate of any of formulae (I)-(III) or (V)-(VII) as
described herein above in the Methods section.
[0301] In other aspects, the present invention is directed to the
use of any one of the compounds of formulae (I)-(III) or (V)-(IX)
as an intermediate in the synthesis of a compound of formula (IV).
For example, in some embodiments, the present invention is directed
to the use of any one of the compounds of formulae (I)-(III) or
(V)-(IX) as an intermediate in the synthesis of a purity-enhanced
or yield-enhanced composition comprising a compound of formula
(IV). In some embodiments, the present invention is directed to the
use of an alpha-enhanced composition comprising a compound of
formula (V) and/or formula (VI) in the synthesis of a
purity-enhanced or yield-enhanced composition comprising a compound
of formula (IV).
Compounds and Compositions Prepared Using Methods of the
Invention
[0302] In some aspects, the present invention is directed to
compounds and compositions prepared using the methods of the
present invention, e.g., alpha-enhanced compositions. In some
embodiments, the compounds and compositions are appropriate for use
in the therapeutic formulations. Such therapeutic formulations can
be, e.g., those described in more detail below. In other
embodiments, the compounds and compositions are appropriate for use
in the synthesis of other products, e.g., compounds of formula
(IV).
Uses of Compositions of the Invention
[0303] In general, the present invention provides compounds useful
for the treatment of inflammatory or immune disorders and the
treatment of cancer, particularly solid tumors. In some
embodiments, the compounds of the invention inhibit NF-.kappa.B
activity, and accordingly may be effective in inflammatory and
immune disorders (see, generally, NF-.kappa.B in Defense and
Disease, J. Clin. Investig 2001, 107, 7). Furthermore, certain
compounds of the invention have also been shown to inhibit receptor
tyrosine kinase activity such as VEGFr and PDGFr in vitro, and are
useful for the treatment of cancer, including solid tumors (see,
Angiogenesis: Potentials for Pharmacologic Intervention in the
Treatment of Cancer, Cardiovascular Diseases, and Chronic
Inflammation, Pharmacological Reviews, 2000, 52, 237).
[0304] Accordingly, in some embodiments, compounds of the present
invention exhibited IC.sub.50 values for NF-.kappa.B inhibition of
less than 10 .mu.M. In certain other embodiments, compounds of the
present invention exhibited IC.sub.50 values less than 7.5 .mu.M.
In certain embodiments, compounds of the present invention
exhibited IC.sub.50 values less than 5 .mu.M, less than 2.5 .mu.M,
less than 1 .mu.M. In certain embodiments, compounds of the present
invention exhibited IC.sub.50 values less than 0.75 .mu.M, less
than 0.5 .mu.M, less than 0.25 .mu.M, less than 0.1 .mu.M, less
than 75 nM, less than 50 nM or even less than 25 nM.
[0305] In still other embodiments, compounds of the present
invention exhibited IC.sub.50 values for inhibition of the growth
of tumor cell lines in vitro of less than 10 .mu.M. In certain
other embodiments, compounds of the present invention exhibited
IC.sub.50 values less than 7.5 .mu.M. In certain embodiments,
compounds of the present invention exhibited IC.sub.50 values less
than 5 .mu.M, less than 2.5 .mu.M, less than 1 .mu.M. In certain
embodiments, compounds of the present invention exhibited IC.sub.50
values less than 0.75 .mu.M, less than 0.5 .mu.M, less than 0.25
.mu.M, less than 0.1 .mu.M, less than 75 nM, less than 50 nM or
even less than 25 nM.
[0306] As discussed above, compounds of the invention exhibit
immunomodulatory activity and exhibit activity for the inhibition
of angiogenesis through inhibition of receptor tyrosine kinases. As
such, the inventive compounds may by useful for the treatment of a
variety of disorders, including, but not limited to, sepsis,
glomerulonephropathy, rheumatoid arthritis (including ankylosing
spondylitis), psoriatic arthritis, osteoarthritis, osteoporosis,
allergic rhinitis, ocular inflammation, inflammatory bowel disease,
atopic dermatitis, psoriasis, asthma, Crohn's disease, ulcerative
colitis, inflammatory pulmonary disease, hepatitis, autoimmune
disorders, diabetes, AIDS, solid tumor cancers, Leukemia,
lymphomas, non-hodgkin's B-cell lymphomas, chronical lymphocytic
leukemia (CLL), multiple myeloma, systemic lupus erythematosus,
allograft rejection/graft versus host disease, eczema, uticaria,
myasthenia gravis, idiopathic thrombocytopenia purpura,
cardiovascular disease (e.g., myocardial infarction,
atherosclerosis), hepatitis, productive nephritis, adenovirus,
diseases/disorders of the central nervous system (stroke,
Alzheimer's disease, epilepsy) and for the treatment of the
symptoms of malaria, to name a few. In certain embodiments,
compounds of the invention are particularly useful for the
treatment of rheumatoid arthritis, psoriasis, multiple sclerosis,
asthma and cancer. In certain embodiments, compounds of the
invention are particularly useful for the treatment of psoriasis.
In certain embodiments, compounds of the invention are particularly
useful for the treatment of cancer. In certain embodiments,
compounds of the invention are particularly useful for the
treatment of atopic dermatitis. Additional information and guidance
for the treatment of such diseases can be found, e.g., in U.S.
Patent Application publication number 2006/0247448, the entire
contents of which are incorporated herein by this reference.
Dosages and Modes of Administration
[0307] It will be appreciated that the compounds and compositions
formed according to the methods of the present invention may be
administered using any amount and any route of administration
effective for the treatment of any of the disease states indicated
herein. Thus, the expression "effective amount" as used herein for
the treatment of cancer, refers to a sufficient amount of agent to
inhibit the growth of tumor cells, or refers to a sufficient amount
to reduce the effects of cancer. The exact amount required will
vary from subject to subject, depending on the species, age, and
general condition of the subject, the severity of the diseases, the
particular anticancer agent, its mode of administration, and the
like. The compounds of the invention are preferably formulated in
dosage unit form for ease of administration and uniformity of
dosage. The expression "dosage unit form" as used herein refers to
a physically discrete unit of therapeutic agent appropriate for the
patient to be treated. It will be understood, however, that the
total daily usage of the compounds and compositions of the present
invention will be decided by the attending physician within the
scope of sound medical judgment. The specific therapeutically
effective dose level for any particular patient or organism will
depend upon a variety of factors including the disorder being
treated and the severity of the disorder; the activity of the
specific compound employed; the specific composition employed; the
age, body weight, general health, sex and diet of the patient; the
time of administration, route of administration, and rate of
excretion of the specific compound employed; the duration of the
treatment; drugs used in combination or coincidental with the
specific compound employed; and like factors well known in the
medical arts (see, for example, Goodman and Gilman's, "The
Pharmacological Basis of Therapeutics", Tenth Edition, A. Gilman,
J. Hardman and L. Limbird, eds., McGraw-Hill Press, 155-173, 2001,
which is incorporated herein by reference in its entirety).
[0308] Pharmaceutical compositions can be administered
systemically, e.g., enteral and parenteral methods of
administration such as intravenous administration, intraperitoneal
administration, intramuscular administration, intracoronary
administration, intraarterial administration (e.g., into a carotid
artery), intradermal administration, subcutaneous administration,
transdermal delivery, intratracheal administration, subcutaneous
administration, intraarticular administration, intraventricular
administration, inhalation (e.g., aerosol), intracerebral, nasal,
naval, oral, intraocular, pulmonary administration, impregnation of
a catheter, by suppository and direct injection into a tissue, or
systemically absorbed topical or mucosal administration. Guidance
for systemic administration of compositions of the present
invention, including appropriate dosage forms, dosages and dosing
schedules can be found, e.g., in U.S. Patent Application
publication number 2006/0247448, the entire contents of which are
incorporated herein by this reference. In certain exemplary
embodiments, the inventive compounds may be used as coating for
stents. Guidance for using compounds of the invention in this
capacity can be found, for example, in WO 05/023792.
EQUIVALENTS
[0309] Those skilled in the art will recognize, or be able to
ascertain using no more than routine experimentation, many
equivalents to the specific embodiments of the invention described
herein. Such equivalents are intended to be encompassed by the
following claims.
INCORPORATION BY REFERENCE
[0310] The contents of all references, patents, and patent
applications cited throughout this application are hereby
incorporated by reference.
EXEMPLIFICATION
[0311] The following naming conventions are used herein:
TABLE-US-00002 TMS trimethylsilyl TMSCL trimethylsilyl chloride
TBDMS t-butyl dimethyl silyl TIPS triisopropylsilyl TBAF tetrabutyl
ammonium fluoride TMSI iodotrimethylsilane PMP p-methoxyphenyl
--OTf triflate BOC t-butyl carbamate MPM 4-methoxybenzyl PCC
pyridinium chlorochromate TBME t-butyl methyl ether TBDPS t-butyl
diphenyl ether DMAP dimethylaminopyridine THF tetrahydrofuran IPA
isopropyl alcohol TBAI tetrabutyl ammonium iodide KOtBu potassium
t-butoxide DMSO dimethyl sulfoxide TBS t-butyl dimethyl silyl TFA
trifluoroacetic anhydride KHMDS potassium bis(trimethylsilyl)amide
DCM dichloromethane
[0312] Amounts of reagents given below are in relation to the first
listed reagent for any given scheme. When volumes are given, they
are calculated using the conversion factor of 1 kg of weight=1 L of
volume.
Example 1
Synthesis of Compound 010
##STR00122##
[0314] D-Ribose (1 wt) was suspended in acetone (5 volumes).
Sulfuric acid (0.05 wts) was added and the mixture was stirred
until homogeneous. Imidazole (0.6 wts) was added. The mixture was
allowed to stir 15 minutes, and then the acetone was distilled off.
Acetone (0.5 vol) was charged in to the reactor. The acetone was
distilled off and the procedure was repeated. Dichloromethane (0.5
vol) was charged into the reactor and distilled off. The material
was taken on crude to the next reaction.
[0315] To the acetonide (1 wts) was added imidazole (0.36 wts). The
mixture was suspended in dichloromethane (5 vols) at 25.degree. C.
The mixture was cooled to 0.degree. C. and tert-butyldiphenylsilyl
chloride (1.4 wts) was added. A saturated solution of ammonium
chloride (2 vols) and water (1 vol) was added and the mixture
stirred 15 minutes. TBME (2.5 vols) was added to the mixture and
stirred 5 minutes. The organic solution was separated and washed
with water (2 vols) and brine (2 vols). The organic layer was
concentrated and the crude TBDPS protected lactol was obtained. The
material was taken crude to the next step.
[0316] To a solution of the TBDPS-acetonide (1 wts) in THF (1.6
vols) at -20.degree. C. was added a solution of vinyl magnesium
bromide (1 M/THF, 5.8 vols) at a rate keeping the temp below
-10.degree. C. The solution was allowed to slowly warm up to
25.degree. C. The reaction was transferred to a cold mixture of
saturated aqueous ammonium chloride (4.5 vols), TBME (4.5 vols) and
water (1.8 vol). The aqueous layer was separated and the organic
solution was washed twice with water (2.25 vols). The organic
solution was washed with brine (4.5 vols) and concentrated. Crude
diol 001 (35.2% yield from d-ribose) was crystallized from heptane.
The solids were collected and washed with cold heptanes, and air
dried.
##STR00123##
[0317] To a stirred solution of diol 001 (1 wts),
dimethylaminopyridine (0.002 wts) and triethylamine (0.76 vols) in
tert-butyl methyl ether (1.75 vols) at 0.degree. C. was added
acetic anhydride (0.46 vols). The solution was allowed to warm to
25.degree. C. and was then stirred for about 1 h. The reaction
mixture was cooled to 0.degree. C. and quenched with saturated
aqueous ammonium chloride (2 vols). TBME (1 vols) was added. The
organic phase was separated and washed with water (2 vols) followed
by brine (2 vols). The combined organic solution was concentrated
to afford diacetate which was used without further
purification.
[0318] A reactor was charged with catalyst Pd(Ph.sub.3P).sub.4
(0.02 wts), triethylamine (0.37 wts) and THF (3 vols). The solution
was cooled to 5.degree. C. Formic acid (0.17 vols) was then added.
After the addition of formic acid was completed, a solution of
diacetate (1 wts) in THF (1 vols) was added. The mixture was heated
to reflux (65.degree. C.) for 2 hours. Upon completion, the mixture
was cooled to 0.degree. C. and quenched with water (1.82 vols).
Tert-butyl methyl ether (2.73 vols) was added and stirred for 15
minutes. After separating the aqueous layer, the solution was
washed with 10 wt % aqueous cysteine (2.0 vols) and then saturated
sodium chloride (1.82 vols). The organic phase was concentrated.
The crude oil residue was dissolved in IPA/water (9:1) (5 vols),
warmed to 70.degree. C. and then cooled to -5.degree. C. The
crystalline acetate 002 was filtered. The cake was washed with cold
IPA/water (9:1) (0.5 vols) and then dried (64.8% yield from 001;
M.P. 64-67.degree. C.). .sup.13C and .sup.1H NMR of 002 are
depicted in FIGS. 3A and 3B.
##STR00124##
[0319] Acetate 002 (1 wt) was suspended in THF (4.7 vol) To the
suspension was added MeOH (2.33 vol). To this resulting suspension
was added a slurry of potassium carbonate (0.3 wts) in MeOH (2.33
vol). The mixture was cooled to 10.degree. C. and then water (8.0
vol) and methyl tert-butyl ether (8 vol) were added. After
agitation (15 minutes), the reaction was allowed to settle (15
minutes) and the organic layer was separated. The organic phase was
then washed with brine (4 vol) and concentrated. The crude alcohol
was used without purification.
[0320] The alcohol (1 wt.) was azeotroped with anhydrous THF
(3.times.1.78 wts) until water levels were <0.03%. To the
resulting oil was added a suspension of TBAI (0.17 wt) in DMF (1.05
wt). The mixture was then cooled to -15.degree. C. The reactor was
then charged with 20 wt % potassium tert-butoxide in THF (1.4
weights) diluted in anhydrous THF (0.94 wt). The mixture was
stirred for 15 minutes. 4-methoxybenzyl chloride (0.43 wt) was then
added. Upon completion, the reaction was quenched with a 0.5M
solution of sodium methoxide in methanol (0.55 weight). The mixture
was then allowed to stir at ambient temperature. The mixture was
concentrated and the remaining oil was partitioned between water (5
weights) and tert-butyl methyl ether (3.7 weights). The organic
layer was washed with saturated aqueous sodium chloride and was
then concentrated. The crude oil was used with purification.
[0321] The silyl ether (1.0 wt) was dissolved in THF (2.3 weights).
Tetrabutylammonium fluoride (1.0 M in THF) (1.9 wts) was added.
Upon completion, 10% aqueous sodium bicarbonate (2.6 wts) was added
and the mixture was extracted twice with tert-butyl methyl ether
(1.9 wts). The combined organic layers were washed with saturated
aqueous sodium chloride (2.6 wts). The organic layer was then
concentrated to provide crude 003.
##STR00125##
[0322] To the crude 003 (1.0 wt based on theoretical mass) was
added TBME (2.95 wts), DMAP (0.04 wts), triethylamine (0.31 wts),
and phthalic anhydride (0.69 wts). Upon completion compound 003a
was extracted with 3% aqueous NaHCO.sub.3 (9.98 weights). The
combined aqueous sodium bicarbonate extractions were returned to
the reactor and then washed twice with heptane (2.27 wts). Sodium
hydroxide (1.23 wts) was then added. Upon conversion back to
compound 003, the aqueous layer was extracted with TBME (2.73 wts).
The TBME organic layers were then concentrated yielding a yellow
orange oil of compound 003 (66.2% yield from 002). .sup.13C and
.sup.1H NMR of 003 are depicted in FIGS. 4A and 4B.
##STR00126##
[0323] Primary alcohol 003 (1 wt.) was dissolved in anhydrous
dichloromethane (5.0 vol.). The solution was cooled to 0.degree.
C., and then diisopropylethylamine (1.38 vol.) was added. Sulfur
trioxide pyridine complex (1.29 wt.) was dissolved in anhydrous
dimethylsulfoxide (5.00 vol.) in a separate reactor. The
SO.sub.3.Py/DMSO solution was added alcohol/CH.sub.2Cl.sub.2
solution. Upon completion, the reaction mixture was quenched with
cold water (6.4 vol.). The organic layer was separated. The aqueous
phase was extracted with a mixture of heptane (4.50 vol.) and
dichloromethane (0.30 vol.). The combined organic phases were
washed with 5 wt % aqueous citric acid (5.0 vol.) until the pH
value of the aqueous layer was .ltoreq.3. The organic phase was
washed with 10 wt % aqueous sodium bicarbonate (2.50 vol.), and
then saturated aqueous sodium chloride (4.80 vol.). The organic
phase was concentrated and dried by azeotroping with heptane
(2.times.4.0 vol.), providing aldehyde 004.
##STR00127##
[0324] Ethyl 3-(S)-hydroxybutyrate (1 wt.) was added to a solution
of 2.0 M lithium diisopropylamide (10 wt.) at 0.degree. C. The
mixture was stirred for 30 minutes, and then was cooled to
-20.degree. C. A solution of methyl iodide (1.8 wt.) in THF (3.4
wt) was added while maintaining a reaction temperature below
-15.degree. C. Upon completion, the reaction was quenched with
saturated aqueous ammonium chloride (8 vol.). The mixture was
extracted twice with ethyl acetate (6 vol. each). The combined
ethyl acetate layers were washed twice with saturated aqueous
sodium chloride (6 vol. each), then concentrated under reduced
pressure. The crude mixture was dried by azeotroping with heptane
and used directly in the next reaction.
[0325] The crude material (1 wt) was dissolved in anhydrous DMF
(3.7 wt.). Imidazole (0.77 wt) and tert-butyldimethylsilyl chloride
(1.25 wt) were added. Upon completion, the reaction was quenched
with water (4 wt.) and extracted twice with heptane (4 wt. each).
The heptane layers were concentrated under reduced pressure, then
solvent exchanged to toluene and used directly in the next
reaction.
[0326] The crude ester (1.0 wt) was dissolved in anhydrous toluene
(1.22 wt.) and the solution was cooled to -10.degree. C. A solution
of diisobutylaluminum hydride in toluene (4.5 wt) was added
maintaining a reaction temperature below 0.degree. C. Upon reaction
completion, methanol (0.4 wt) was added. The reaction mixture was
transferred into a solution of cold aqueous hydrochloric acid (6.0
wt). The mixture was extracted twice with methyl tert-butyl ether
(2.3 wt. each). The combined organic layers were concentrated under
reduced pressure. The crude product 005 (68.7% yield from Ethyl
3-(S)-hydroxybutyrate starting material) was then purified by
vacuum distillation (100-120.degree. C. at 10 torr).
[0327] The alcohol 005 (1.0 wt) was dissolved in THF (3 wt) and
cooled to 0.degree. C. Triethylamine (0.51 wt) was added followed
by methanesulfonyl chloride (0.55 wt.). Upon completion of the
reaction, water (2.5 wt) was added followed by heptane (3.5 wt.).
After phase separation, the heptane layer was washed with saturated
sodium chloride (2.5 wt) and then concentrated under reduced
pressure. The crude material was used directly in the next
reaction.
[0328] The mesylate (1.0 wt) was dissolved in acetone (3.33 wt).
Sodium iodide (1.0 wt) was added and the mixture was heated to
reflux. Upon completion, the reaction was cooled to ambient
temperature and water (2.8 wt) was added. The mixture was extracted
with heptane (4.0 vol). The heptane layer was washed consecutively
with saturated aqueous sodium bicarbonate (1 wt), saturated aqueous
sodium thiosulfate (2.5 wt) and saturated aqueous sodium chloride
(2.0 wt). The heptane layer was concentrated under reduced pressure
and used directly in the next reaction.
[0329] Triphenylphosphine (3.0 wt.) was heated to 100.degree. C.
The iodide (1.0 wt) was added and the mixture was stirred at
100.degree. C. until the iodide was consumed. Ethyl acetate (5 wt)
was added and the mixture was maintained at reflux for 20 minutes,
and then cooled to 0.degree. C. The resulting solid phosphonium
salt 006 (72.4% yield from 005) was filtered, rinsed with
additional ethyl acetate (7 vol) and then dried under nitrogen.
.sup.13C and .sup.1H NMR of 006 are depicted in FIGS. 5A and
5B.
##STR00128##
[0330] Phosphonium salt 006 (2.40 wts) was dried by azeotroping
with anhydrous THF (9.60 vol.). Anhydrous THF was added (4.80 vol)
and the mixture was cooled to 0.degree. C. 1.6 M n-butyl lithium
(2.42 vol.) was added, and the solution was stirred for 20 min. A
solution of aldehyde 004 (1 wt.) in anhydrous THF (1 vol.) was
added to the reaction mixture and the reaction was warmed to
20.degree. C. Upon completion, Celite (1.3 wt.) was added followed
by a solution of citric acid/D.I. water (0.13 wt./0.15 vol.).
Heptane (3.90 vol.) was added and the mixture was filtered and
washed with heptane (2.times.5.23 vol.). The combined filtrates
were concentrated. Heptane (3.78 vol.) was added and the solution
was filtered, rinsing with heptane (2.times.3.78 vol.). The
combined filtrates were concentrated and the crude material was
used without purification. .sup.13C and .sup.1H NMR of 007 are
depicted in FIGS. 6A and 6B.
##STR00129##
[0331] The triflate 007a (1.1 wt), olefin 007 (1.0 wt), and
tris(dibenzylideneacetone)-dipalladium were combined in a reactor.
N-methylpyrrolidinone (3.3 vol) and dicyclohexylmethylamine (0.77
wt) were added. The mixture was stirred at 80.degree. C. Upon
completion, the mixture was cooled to 20.degree. C., and Celite
(1.5 wt) and ethyl acetate (10 vol) were added. The mixture was
filtered and the solids rinsed with ethyl acetate (30 vol). The
filtrates were concentrated under reduced pressure. The crude
concentrate was purified by silica gel chromatography, which
afforded a 72.5% yield.
##STR00130##
[0332] The Boc-amide 008 was dissolved in
dimethyltetrahydropyrimidinone and cooled to 0.degree. C. A 1.0 M
solution of lithium bis(trimethylsilylamide) in THF (2.5 vol) was
added. Iodoethane (1.2 wt) was added, then the mixture was warmed
to 0.degree. C. Upon completion, the mixture was cooled to
0.degree. C. and was quenched with saturated aqueous ammonium
chloride (25 vol). The mixture was extracted three times with a
mixture of heptane (4.0 vol) and methyl tert-butyl ether (4.0 vol).
The combined organic layers were washed with saturated aqueous
sodium chloride (25 vol) and then concentrated under reduced
pressure. The crude silyl ether concentrate was purified by silica
gel chromatography, which afforded a 94% yield.
[0333] Imidazole hydrochloride (0.44 wt) was dissolved in 1.0 M
tetrabutylammonium fluoride in THF (8.5 vol). A solution of the
silyl ether (1.0 wt) in THF (4.8 vol) was added. Upon completion,
the mixture was quenched with saturated aqueous ammonium chloride
(15.0 vol) and extracted three times with methyl tert-butyl ether
(10 vol each). The combined organic layers were washed with water
(33 vol) and saturated aqueous sodium chloride (33 vol), and then
concentrated under reduced pressure. The crude alcohol concentrate
was purified by silica gel chromatography, which afforded a 51.6%
yield.
[0334] The alcohol (1.0 wt) was dissolved in THF (47 vol) and the
solution was cooled to 0.degree. C. A 0.5 M solution of potassium
bis(trimethylsilyl)amide (3.0 vol) was added over a 3 hour period.
Upon completion, a solution of tert-butyldimethylsilyl chloride
(1.0 wt) in THF (1.0 vol) was added. Upon completion, saturated
aqueous ammonium chloride (25 vol) and water (4 vol) were added.
The mixture was extracted with tert-butyl methyl ether (25 vol).
After removal of the aqueous layer, the organic layer was washed
with saturated aqueous sodium chloride (25 vol). The combined
organic layer was concentrated under reduced pressure. The crude
concentrate was purified by silica gel chromatography, providing
compound 009 (57.4% yield)..sup.13C and .sup.1H NMR of 009 are
depicted in FIGS. 7A and 7B.
##STR00131##
[0335] A solution of macrocycle 009 (1 wt.) in dichloromethane (2.7
vol.) was added to a reactor containing dichlorodicyanobenzoquinone
(DDQ, 0.35 wt) and water (0.6 vol). Upon completion, the reaction
was quenched with saturated aqueous sodium bicarbonate (4.0 vol)
and saturated sodium thoisulfate (1 vol). After phase separation,
the aqueous layer was extracted with a mixture of ethyl acetate
(1.3 vol) and heptane (2.6 vol). The combined organic layers were
concentrated under reduced pressure. The crude concentrate was
dissolved in ethyl acetate (1.6 vol) and the solution was added to
a solution of semicarbazide hydrochloride (0.22 wt) and sodium
acetate (0.54 wt) in water (0.96 vol). Upon completion, the solids
were filtered and rinsed with ethyl acetate (3.34 vol). The aqueous
phase was removed from the filtrates and the organic layer was
concentrated under reduced pressure. The concentrate was dissolved
in a mixture of heptane (2 vol) and dichloromethane (2.0 vol),
polish filtered, and concentrated under reduced pressure, providing
the allylic alcohol.
[0336] The allylic alcohol (1.0 wt) was dissolved in
dichloromethane (4.0 vol). Dess-Martin periodinane (0.68 wt.) was
added portionwise. Upon completion, the reaction was quenched with
saturated aqueous sodium bicarbonate (7.0 vol.). A solution of 10
wt % aqueous sodium thiosulfate (5.5 vol.) was added. The organic
phase was separated and the aqueous layer was extracted with a
mixture of ethyl acetate (2.2 vol) and heptane (2.2 vol). The
combined organic phases were concentrated under reduced pressure.
The concentrate was dissolved in ethyl acetate (4.5 vol.), and was
washed with saturated aqueous sodium chloride (1.5 vol.). The
organic phase was concentrated under reduced pressure, providing
the enone.
[0337] A solution of the enone (1.0 wt) in dichloromethane (6.5
vol) was added to a 0.degree. C. solution of trifluoroacetic acid
(6.1 vol) in water (0.3 vol). Upon completion, the reaction was
quenched with chilled (0.degree. C.) 15.0% aqueous ammonium
hydroxide (11.0 vol). After separation of the organic layer, the
aqueous layer was extracted with dichloromethane (2.3 vol). The
combined organic layers were washed with saturated sodium chloride
(4.6 vol). The organic phase was concentrated under reduced
pressure. t-Butyl methyl ether (2.2 vol) was added and the mixture
was warmed to 55.degree. C. Heptane (2.2 vol) was gradually added,
then the solution was cooled to 0.degree. C. The solids were
filtered and washed with a 0.degree. C. mixture of heptane (1.4
vol) and t-butyl methyl ether (1.4 vol). The solid 010 was dried
and then suspended in 2-propanol (5.0 vol) and warmed to 65.degree.
C. Water (0.1 vol) was added followed by gradual addition of
heptane (7.5 vol). The mixture was then cooled to 0.degree. C. and
the solids of 010 (80.7% yield from 009; M.P. 157-159.degree. C.)
were filtered, rinsed with a mixture of heptane (2.4 vol) and
2-propanol (2.4 vol), and then dried. .sup.13C and .sup.1H NMR of
010 are depicted in FIGS. 8A and 8B.
Example 2
Synthesis of Compound 010
##STR00132##
[0339] Compound 007 was synthesized from compound 003 as described
above in Example 1.
##STR00133##
[0340] The TBS ether 007 (1 wt) was dissolved in THF (0.88 wt.). A
1.0 M solution of tetrabutylammonium fluoride in THF (2.1 wt.) was
added. The solution was warmed to 50.degree. C. Upon completion,
the mixture was cooled to 20.degree. C. 10 wt % aqueous sodium
bicarbonate (3 vol) was added and the mixture was extracted with
tert-butyl methyl ether (6 vol). The organic layer was washed with
saturated aqueous sodium chloride (3 vol) and was then
concentrated. The crude material was used without purification.
[0341] 4-(dimethylamino)pyridine (0.03 wts) was added to a solution
of the alcohol (1 wt) in anhydrous THF (9 vol.). Triethylamine (0.3
wts) was added and then 4-nitrobenzoyl chloride (0.5 wts) as a
solution in THF (1.0 vol.). The reaction mixture was then stirred
at 35.degree. C. Upon completion, the reaction was cooled to
20.degree. C. 5 wt % aqueous sodium bicarbonate (10 vol) was added
followed by t-butyl methyl ether (15 vol). The organic phase was
washed with 20 wt % aqueous sodium chloride (10 vol). The organic
phase was concentrated and the solvent exchanged to methanol.
Methanol (6 vol.) was added and the mixture was warmed to
50.degree. C., followed by stirring at 50.degree. C. for 30
minutes, and then cooling to 0.degree. C. The crystalline solid
(56.1% yield; M.P. 86-89.degree. C.) was filtered, washed with cold
methanol and dried. A single crystal of compound 011 was isolated,
and the crystal structure is shown in FIG. 1. .sup.13C and .sup.1H
NMR of 011 are depicted in FIGS. 9A and 9B.
##STR00134##
[0342] The p-nitrobenzoate ester 011 (1.0 wt) was dissolved in THF
(2.65 wt) and methanol (0.4 wt). A 10 wt % aqueous sodium hydroxide
solution (1.65 wt) was added and the mixture was warmed to
35.degree. C. Upon completion, the reaction was cooled to
20.degree. C. Water (3.0 vol) was added followed by methyl
tert-butyl ether (6 vol). After separation, the organic layer was
washed with 25 wt % sodium chloride (4 vol). The solvent was
removed under reduced pressure.
[0343] The aryl triflate 011a [1.26 wt.; prepared by treating 007a
with LiHDMS and DMPU followed by a quench with Ethyl iodide;
.sup.13C and .sup.1H NMR depicted in FIGS. 11A and 11B; single
crystal X-ray depicted in FIG. 2] and
tris(dibenzylidineacetone)dipalladium (0.12 wt.) were combined in a
reactor. A solution of the olefin (1.0 wt) in N-methylpyrrolidinone
(1.6 wt) was added followed by N-methyldicyclohexylamine (0.53
wt.). The mixture was heated to 80.degree. C. Upon completion, the
mixture was cooled to 20.degree. C. A slurry of Celite (0.5 wt.) in
MTBE (3.70 wt.) was added. The mixture was filtered and the solids
were rinsed 3 times with MTBE (3.70 wt. each). The combined
filtrates were washed with 1N aqueous hydrochloric acid (5.1 wt.),
twice with 5 wt % aqueous L-cysteine (5 wt.), then 25 wt % aqueous
sodium chloride (5.33 wt.). The solvent was concentrate under
reduced pressure, providing compound 012. .sup.13C and .sup.1H NMR
of 012 are depicted in FIGS. 10A and 10B.
##STR00135##
[0344] The alcohol 012 (1 wt.) was dissolved in THF (23 vol) and
added to a cold solution of 20 wt % potassium tert-butoxide in THF
(0.88 vol) diluted with THF (19.7 vol.). Upon completion, a
solution of tert-butyldimethylsilyl chloride (0.33 wt.) in THF
(0.28 vol.) was added. Saturated aqueous sodium bicarbonate (3.5
vol.) was then added, and the solvent was removed under reduced
pressure. The residue was dissolved with tert-butyl methyl ether (7
vol.) and the aqueous phase was separated. The organic phase was
washed with 25 wt % aqueous sodium chloride (8.4 vol.). The solvent
was evaporated under reduced pressure, providing macrocycle
009.
##STR00136##
[0345] Compound 010 was synthesized from compound 009 as described
above in Example 1.
Example 3
Deprotection of 009
##STR00137##
[0347] To a solution of enone 009 (1.0 wt) in dichloromethane (2.0
vol) and methanol (2.0 vol) was added potassium fluoride (0.16 wt).
Upon completion, water (4.0 wt) and methyl tert-butyl ether (4.0
vol) were added. After separation of the aqueous layer, the organic
phase was washed with 25 wt % aqueous sodium chloride, then
concentrated under reduced pressure. The crude concentrate was
solvent exchanged with 2-propanol (2.0 wt). The crude product 009a
was slurried into methanol (5 vol) and heated to 65.degree. C. The
solution was cooled to -20.degree. C. The solids (67% yield; M.P.
174-175.degree. C.) were filtered and washed with methanol (5.0
vol) which was pre-chilled to -20.degree. C. [Overall yield from
011 was 29%]. .sup.13C and .sup.1H NMR of 009a are depicted in
FIGS. 12A and 12B.
[0348] To a solution of phenol 009a (1.0 wt) in dichloromethane
(8.0 wt) and water (0.2 wt) was added trifluoromethanesulfonic acid
(0.51 wt). The reaction mixture was warmed to 30.degree. C. Upon
completion, the reaction was quenched with aqueous sodium
bicarbonate (5.0 wt), and tert-butyl methyl ether (6.0 wt) was
added. The aqueous layer was removed. The organic phase was washed
with water (4.0 wt) and 25 wt % aqueous sodium chloride (4.0 wt).
The organic phase was polish filtered, then concentrated under
reduced pressure. The crude product was solvent exchanged with
2-propanol (2.0 wt) and concentrated to dryness. The crude solid
was slurried with 2-propanol (10 vol.). The mixture was heated to
65.degree. C. The solution was then cooled to a temperature of
40.degree. C. and seed crystals were added. The mixture was then
cooled to 0.degree. C., and then the solids were filtered. The
solids were rinsed with 2-propanol (2.0 vol) pre-chilled to
-20.degree. C. The cake was then dried, providing compound 010 in
72.6% yield (M.P. 157-159.degree. C.; Optical rotation+47.degree.
at 5 mg/mL in MeOH).
* * * * *