U.S. patent application number 13/582366 was filed with the patent office on 2013-05-09 for expression vector. This patent application is currently assigned to F. Hoffmann-La Roche AG. The applicant listed for this patent is Jeremy Beauchamp, Anita Dreyer, Hugues Matile. Invention is credited to Jeremy Beauchamp, Anita Dreyer, Hugues Matile.
Application Number | 20130115219 13/582366 |
Document ID | / |
Family ID | 44168056 |
Filed Date | 2013-05-09 |
United States Patent Application | 20130115219 |
Kind Code | A1 |
Beauchamp; Jeremy ; et al. | May 9, 2013 |
The present invention provides an expression vector for cell-surface expression of proteins.
Inventors: | Beauchamp; Jeremy; (Riehen, CH) ; Dreyer; Anita; (Basel, CH) ; Matile; Hugues; (Basel, CH) | ||||||||||
Applicant: |
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Assignee: | F. Hoffmann-La Roche AG Basel CH |
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Family ID: | 44168056 | ||||||||||
Appl. No.: | 13/582366 | ||||||||||
Filed: | February 28, 2011 | ||||||||||
PCT Filed: | February 28, 2011 | ||||||||||
PCT NO: | PCT/EP2011/052873 | ||||||||||
371 Date: | August 31, 2012 |
Current U.S. Class: | 424/141.1 ; 435/320.1; 435/369; 435/69.3; 435/7.92 |
Current CPC Class: | C07K 2319/03 20130101; C07K 2319/036 20130101; C12N 15/85 20130101; C07K 16/205 20130101; C07K 2319/21 20130101; C07K 2317/14 20130101; C07K 2319/40 20130101; C07K 14/445 20130101; C07K 2319/43 20130101; C07K 2319/02 20130101; C12N 2810/855 20130101; G01N 33/56905 20130101 |
Class at Publication: | 424/141.1 ; 435/320.1; 435/369; 435/69.3; 435/7.92 |
International Class: | C12N 15/85 20060101 C12N015/85 |
Date | Code | Application Number |
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Mar 2, 2010 | EP | 10155115.8 |
Sequence CWU 1
1
15166PRTMus musculus 1His Asp Phe Pro Ala Leu Val Met Ile Leu Ile
Ile Leu Gly Val Met 1 5 10 15 Ala Gly Ile Ile Gly Thr Ile Leu Leu
Ile Ser Tyr Cys Ile Ser Arg 20 25 30 Met Thr Lys Lys Ser Ser Val
Asp Ile Gln Ser Pro Glu Gly Gly Asp 35 40 45 Asn Ser Val Pro Leu
Ser Ser Ile Glu Gln Thr Pro Asn Glu Glu Ser 50 55 60 Ser Asn 65
221PRTApis mellifera 2Met Lys Phe Leu Val Asn Val Ala Leu Val Phe
Met Val Val Tyr Ile 1 5 10 15 Ser Phe Ile Tyr Ala 20
38PRTArtificial SequenceFLAG tag 3Asp Tyr Lys Asp Asp Asp Asp Lys 1
5 410PRTArtificial SequenceHis Tag 4Val Ser Gly Gly His His His His
His His 1 5 10 5390DNAArtificial SequenceExpression construct
5atgaagttcc tggtgaatgt ggccttggtg ttcatggtgg tgtacatcag cttcatctac
60gctagcctta agggtaccga gctcggatcc actagtgaat tcgatatcga cgtcgcggcc
120gctgactaca aagacgatga cgacaagagc cctagggccg cacacgattt
tcctgcacta 180gtgatgatac tcataatttt gggcgtgatg gcagggatta
tcggaactat ccttcttatc 240tcttactgta tcagccgaat gacaaagaaa
agttcagttg acatccaatc tcctgagggt 300ggtgacaaca gtgtgccttt
gagttctatt gagcagactc ctaatgaaga gtcctccaat 360gttagcggcg
gccatcacca tcaccatcac 39067140DNAHomo sapiensCDS(314)..(7099)
6gtaattgcga gcgagagtga gtggggccgg gacccgcaga gccgagccga cccttctctc
60ccgggctgcg gcagggcagg gcggggagct ccgcgcacca acagagccgg ttctcagggc
120gctttgctcc ttgttttttc cccggttctg ttttctcccc ttctccggaa
ggcttgtcaa 180ggggtaggag aaagagacgc aaacacaaaa gtggaaaaca
gttaatgacc agccacggcg 240tccctgctgt gagctctggc cgctgccttc
cagggctccc gagccacacg ctgggggtgc 300tggctgaggg aac atg gct tgt tgg
cct cag ctg agg ttg ctg ctg tgg 349 Met Ala Cys Trp Pro Gln Leu Arg
Leu Leu Leu Trp 1 5 10 aag aac ctc act ttc aga aga aga caa aca tgt
cag ctg ctg ctg gaa 397Lys Asn Leu Thr Phe Arg Arg Arg Gln Thr Cys
Gln Leu Leu Leu Glu 15 20 25 gtg gcc tgg cct cta ttt atc ttc ctg
atc ctg atc tct gtt cgg ctg 445Val Ala Trp Pro Leu Phe Ile Phe Leu
Ile Leu Ile Ser Val Arg Leu 30 35 40 agc tac cca ccc tat gaa caa
cat gaa tgc cat ttt cca aat aaa gcc 493Ser Tyr Pro Pro Tyr Glu Gln
His Glu Cys His Phe Pro Asn Lys Ala 45 50 55 60 atg ccc tct gca gga
aca ctt cct tgg gtt cag ggg att atc tgt aat 541Met Pro Ser Ala Gly
Thr Leu Pro Trp Val Gln Gly Ile Ile Cys Asn 65 70 75 gcc aac aac
ccc tgt ttc cgt tac ccg act cct ggg gag gct ccc gga 589Ala Asn Asn
Pro Cys Phe Arg Tyr Pro Thr Pro Gly Glu Ala Pro Gly 80 85 90 gtt
gtt gga aac ttt aac aaa tcc att gtg gct cgc ctg ttc tca gat 637Val
Val Gly Asn Phe Asn Lys Ser Ile Val Ala Arg Leu Phe Ser Asp 95 100
105 gct cgg agg ctt ctt tta tac agc cag aaa gac acc agc atg aag gac
685Ala Arg Arg Leu Leu Leu Tyr Ser Gln Lys Asp Thr Ser Met Lys Asp
110 115 120 atg cgc aaa gtt ctg aga aca tta cag cag atc aag aaa tcc
agc tca 733Met Arg Lys Val Leu Arg Thr Leu Gln Gln Ile Lys Lys Ser
Ser Ser 125 130 135 140 aac ttg aag ctt caa gat ttc ctg gtg gac aat
gaa acc ttc tct ggg 781Asn Leu Lys Leu Gln Asp Phe Leu Val Asp Asn
Glu Thr Phe Ser Gly 145 150 155 ttc ctg tat cac aac ctc tct ctc cca
aag tct act gtg gac aag atg 829Phe Leu Tyr His Asn Leu Ser Leu Pro
Lys Ser Thr Val Asp Lys Met 160 165 170 ctg agg gct gat gtc att ctc
cac aag gta ttt ttg caa ggc tac cag 877Leu Arg Ala Asp Val Ile Leu
His Lys Val Phe Leu Gln Gly Tyr Gln 175 180 185 tta cat ttg aca agt
ctg tgc aat gga tca aaa tca gaa gag atg att 925Leu His Leu Thr Ser
Leu Cys Asn Gly Ser Lys Ser Glu Glu Met Ile 190 195 200 caa ctt ggt
gac caa gaa gtt tct gag ctt tgt ggc cta cca agg gag 973Gln Leu Gly
Asp Gln Glu Val Ser Glu Leu Cys Gly Leu Pro Arg Glu 205 210 215 220
aaa ctg gct gca gca gag cga gta ctt cgt tcc aac atg gac atc ctg
1021Lys Leu Ala Ala Ala Glu Arg Val Leu Arg Ser Asn Met Asp Ile Leu
225 230 235 aag cca atc ctg aga aca cta aac tct aca tct ccc ttc ccg
agc aag 1069Lys Pro Ile Leu Arg Thr Leu Asn Ser Thr Ser Pro Phe Pro
Ser Lys 240 245 250 gag ctg gct gaa gcc aca aaa aca ttg ctg cat agt
ctt ggg act ctg 1117Glu Leu Ala Glu Ala Thr Lys Thr Leu Leu His Ser
Leu Gly Thr Leu 255 260 265 gcc cag gag ctg ttc agc atg aga agc tgg
agt gac atg cga cag gag 1165Ala Gln Glu Leu Phe Ser Met Arg Ser Trp
Ser Asp Met Arg Gln Glu 270 275 280 gtg atg ttt ctg acc aat gtg aac
agc tcc agc tcc tcc acc caa atc 1213Val Met Phe Leu Thr Asn Val Asn
Ser Ser Ser Ser Ser Thr Gln Ile 285 290 295 300 tac cag gct gtg tct
cgt att gtc tgc ggg cat ccc gag gga ggg ggg 1261Tyr Gln Ala Val Ser
Arg Ile Val Cys Gly His Pro Glu Gly Gly Gly 305 310 315 ctg aag atc
aag tct ctc aac tgg tat gag gac aac aac tac aaa gcc 1309Leu Lys Ile
Lys Ser Leu Asn Trp Tyr Glu Asp Asn Asn Tyr Lys Ala 320 325 330 ctc
ttt gga ggc aat ggc act gag gaa gat gct gaa acc ttc tat gac 1357Leu
Phe Gly Gly Asn Gly Thr Glu Glu Asp Ala Glu Thr Phe Tyr Asp 335 340
345 aac tct aca act cct tac tgc aat gat ttg atg aag aat ttg gag tct
1405Asn Ser Thr Thr Pro Tyr Cys Asn Asp Leu Met Lys Asn Leu Glu Ser
350 355 360 agt cct ctt tcc cgc att atc tgg aaa gct ctg aag ccg ctg
ctc gtt 1453Ser Pro Leu Ser Arg Ile Ile Trp Lys Ala Leu Lys Pro Leu
Leu Val 365 370 375 380 ggg aag atc ctg tat aca cct gac act cca gcc
aca agg cag gtc atg 1501Gly Lys Ile Leu Tyr Thr Pro Asp Thr Pro Ala
Thr Arg Gln Val Met 385 390 395 gct gag gtg aac aag acc ttc cag gaa
ctg gct gtg ttc cat gat ctg 1549Ala Glu Val Asn Lys Thr Phe Gln Glu
Leu Ala Val Phe His Asp Leu 400 405 410 gaa ggc atg tgg gag gaa ctc
agc ccc aag atc tgg acc ttc atg gag 1597Glu Gly Met Trp Glu Glu Leu
Ser Pro Lys Ile Trp Thr Phe Met Glu 415 420 425 aac agc caa gaa atg
gac ctt gtc cgg atg ctg ttg gac agc agg gac 1645Asn Ser Gln Glu Met
Asp Leu Val Arg Met Leu Leu Asp Ser Arg Asp 430 435 440 aat gac cac
ttt tgg gaa cag cag ttg gat ggc tta gat tgg aca gcc 1693Asn Asp His
Phe Trp Glu Gln Gln Leu Asp Gly Leu Asp Trp Thr Ala 445 450 455 460
caa gac atc gtg gcg ttt ttg gcc aag cac cca gag gat gtc cag tcc
1741Gln Asp Ile Val Ala Phe Leu Ala Lys His Pro Glu Asp Val Gln Ser
465 470 475 agt aat ggt tct gtg tac acc tgg aga gaa gct ttc aac gag
act aac 1789Ser Asn Gly Ser Val Tyr Thr Trp Arg Glu Ala Phe Asn Glu
Thr Asn 480 485 490 cag gca atc cgg acc ata tct cgc ttc atg gag tgt
gtc aac ctg aac 1837Gln Ala Ile Arg Thr Ile Ser Arg Phe Met Glu Cys
Val Asn Leu Asn 495 500 505 aag cta gaa ccc ata gca aca gaa gtc tgg
ctc atc aac aag tcc atg 1885Lys Leu Glu Pro Ile Ala Thr Glu Val Trp
Leu Ile Asn Lys Ser Met 510 515 520 gag ctg ctg gat gag agg aag ttc
tgg gct ggt att gtg ttc act gga 1933Glu Leu Leu Asp Glu Arg Lys Phe
Trp Ala Gly Ile Val Phe Thr Gly 525 530 535 540 att act cca ggc agc
att gag ctg ccc cat cat gtc aag tac aag atc 1981Ile Thr Pro Gly Ser
Ile Glu Leu Pro His His Val Lys Tyr Lys Ile 545 550 555 cga atg gac
att gac aat gtg gag agg aca aat aaa atc aag gat ggg 2029Arg Met Asp
Ile Asp Asn Val Glu Arg Thr Asn Lys Ile Lys Asp Gly 560 565 570 tac
tgg gac cct ggt cct cga gct gac ccc ttt gag gac atg cgg tac 2077Tyr
Trp Asp Pro Gly Pro Arg Ala Asp Pro Phe Glu Asp Met Arg Tyr 575 580
585 gtc tgg ggg ggc ttc gcc tac ttg cag gat gtg gtg gag cag gca atc
2125Val Trp Gly Gly Phe Ala Tyr Leu Gln Asp Val Val Glu Gln Ala Ile
590 595 600 atc agg gtg ctg acg ggc acc gag aag aaa act ggt gtc tat
atg caa 2173Ile Arg Val Leu Thr Gly Thr Glu Lys Lys Thr Gly Val Tyr
Met Gln 605 610 615 620 cag atg ccc tat ccc tgt tac gtt gat gac atc
ttt ctg cgg gtg atg 2221Gln Met Pro Tyr Pro Cys Tyr Val Asp Asp Ile
Phe Leu Arg Val Met 625 630 635 agc cgg tca atg ccc ctc ttc atg acg
ctg gcc tgg att tac tca gtg 2269Ser Arg Ser Met Pro Leu Phe Met Thr
Leu Ala Trp Ile Tyr Ser Val 640 645 650 gct gtg atc atc aag ggc atc
gtg tat gag aag gag gca cgg ctg aaa 2317Ala Val Ile Ile Lys Gly Ile
Val Tyr Glu Lys Glu Ala Arg Leu Lys 655 660 665 gag acc atg cgg atc
atg ggc ctg gac aac agc atc ctc tgg ttt agc 2365Glu Thr Met Arg Ile
Met Gly Leu Asp Asn Ser Ile Leu Trp Phe Ser 670 675 680 tgg ttc att
agt agc ctc att cct ctt ctt gtg agc gct ggc ctg cta 2413Trp Phe Ile
Ser Ser Leu Ile Pro Leu Leu Val Ser Ala Gly Leu Leu 685 690 695 700
gtg gtc atc ctg aag tta gga aac ctg ctg ccc tac agt gat ccc agc
2461Val Val Ile Leu Lys Leu Gly Asn Leu Leu Pro Tyr Ser Asp Pro Ser
705 710 715 gtg gtg ttt gtc ttc ctg tcc gtg ttt gct gtg gtg aca atc
ctg cag 2509Val Val Phe Val Phe Leu Ser Val Phe Ala Val Val Thr Ile
Leu Gln 720 725 730 tgc ttc ctg att agc aca ctc ttc tcc aga gcc aac
ctg gca gca gcc 2557Cys Phe Leu Ile Ser Thr Leu Phe Ser Arg Ala Asn
Leu Ala Ala Ala 735 740 745 tgt ggg ggc atc atc tac ttc acg ctg tac
ctg ccc tac gtc ctg tgt 2605Cys Gly Gly Ile Ile Tyr Phe Thr Leu Tyr
Leu Pro Tyr Val Leu Cys 750 755 760 gtg gca tgg cag gac tac gtg ggc
ttc aca ctc aag atc ttc gct agc 2653Val Ala Trp Gln Asp Tyr Val Gly
Phe Thr Leu Lys Ile Phe Ala Ser 765 770 775 780 ctg ctg tct cct gtg
gct ttt ggg ttt ggc tgt gag tac ttt gcc ctt 2701Leu Leu Ser Pro Val
Ala Phe Gly Phe Gly Cys Glu Tyr Phe Ala Leu 785 790 795 ttt gag gag
cag ggc att gga gtg cag tgg gac aac ctg ttt gag agt 2749Phe Glu Glu
Gln Gly Ile Gly Val Gln Trp Asp Asn Leu Phe Glu Ser 800 805 810 cct
gtg gag gaa gat ggc ttc aat ctc acc act tcg gtc tcc atg atg 2797Pro
Val Glu Glu Asp Gly Phe Asn Leu Thr Thr Ser Val Ser Met Met 815 820
825 ctg ttt gac acc ttc ctc tat ggg gtg atg acc tgg tac att gag gct
2845Leu Phe Asp Thr Phe Leu Tyr Gly Val Met Thr Trp Tyr Ile Glu Ala
830 835 840 gtc ttt cca ggc cag tac gga att ccc agg ccc tgg tat ttt
cct tgc 2893Val Phe Pro Gly Gln Tyr Gly Ile Pro Arg Pro Trp Tyr Phe
Pro Cys 845 850 855 860 acc aag tcc tac tgg ttt ggc gag gaa agt gat
gag aag agc cac cct 2941Thr Lys Ser Tyr Trp Phe Gly Glu Glu Ser Asp
Glu Lys Ser His Pro 865 870 875 ggt tcc aac cag aag aga ata tca gaa
atc tgc atg gag gag gaa ccc 2989Gly Ser Asn Gln Lys Arg Ile Ser Glu
Ile Cys Met Glu Glu Glu Pro 880 885 890 acc cac ttg aag ctg ggc gtg
tcc att cag aac ctg gta aaa gtc tac 3037Thr His Leu Lys Leu Gly Val
Ser Ile Gln Asn Leu Val Lys Val Tyr 895 900 905 cga gat ggg atg aag
gtg gct gtc gat ggc ctg gca ctg aat ttt tat 3085Arg Asp Gly Met Lys
Val Ala Val Asp Gly Leu Ala Leu Asn Phe Tyr 910 915 920 gag ggc cag
atc acc tcc ttc ctg ggc cac aat gga gcg ggg aag acg 3133Glu Gly Gln
Ile Thr Ser Phe Leu Gly His Asn Gly Ala Gly Lys Thr 925 930 935 940
acc acc atg tca atc ctg acc ggg ttg ttc ccc ccg acc tcg ggc acc
3181Thr Thr Met Ser Ile Leu Thr Gly Leu Phe Pro Pro Thr Ser Gly Thr
945 950 955 gcc tac atc ctg gga aaa gac att cgc tct gag atg agc acc
atc cgg 3229Ala Tyr Ile Leu Gly Lys Asp Ile Arg Ser Glu Met Ser Thr
Ile Arg 960 965 970 cag aac ctg ggg gtc tgt ccc cag cat aac gtg ctg
ttt gac atg ctg 3277Gln Asn Leu Gly Val Cys Pro Gln His Asn Val Leu
Phe Asp Met Leu 975 980 985 act gtc gaa gaa cac atc tgg ttc tat gcc
cgc ttg aaa ggg ctc tct 3325Thr Val Glu Glu His Ile Trp Phe Tyr Ala
Arg Leu Lys Gly Leu Ser 990 995 1000 gag aag cac gtg aag gcg gag
atg gag cag atg gcc ctg gat gtt 3370Glu Lys His Val Lys Ala Glu Met
Glu Gln Met Ala Leu Asp Val 1005 1010 1015 ggt ttg cca tca agc aag
ctg aaa agc aaa aca agc cag ctg tca 3415Gly Leu Pro Ser Ser Lys Leu
Lys Ser Lys Thr Ser Gln Leu Ser 1020 1025 1030 ggt gga atg cag aga
aag cta tct gtg gcc ttg gcc ttt gtc ggg 3460Gly Gly Met Gln Arg Lys
Leu Ser Val Ala Leu Ala Phe Val Gly 1035 1040 1045 gga tct aag gtt
gtc att ctg gat gaa ccc aca gct ggt gtg gac 3505Gly Ser Lys Val Val
Ile Leu Asp Glu Pro Thr Ala Gly Val Asp 1050 1055 1060 cct tac tcc
cgc agg gga ata tgg gag ctg ctg ctg aaa tac cga 3550Pro Tyr Ser Arg
Arg Gly Ile Trp Glu Leu Leu Leu Lys Tyr Arg 1065 1070 1075 caa ggc
cgc acc att att ctc tct aca cac cac atg gat gaa gcg 3595Gln Gly Arg
Thr Ile Ile Leu Ser Thr His His Met Asp Glu Ala 1080 1085 1090 gac
gtc ctg ggg gac agg att gcc atc atc tcc cat ggg aag ctg 3640Asp Val
Leu Gly Asp Arg Ile Ala Ile Ile Ser His Gly Lys Leu 1095 1100 1105
tgc tgt gtg ggc tcc tcc ctg ttt ctg aag aac cag ctg gga aca 3685Cys
Cys Val Gly Ser Ser Leu Phe Leu Lys Asn Gln Leu Gly Thr 1110 1115
1120 ggc tac tac ctg acc ttg gtc aag aaa gat gtg gaa tcc tcc ctc
3730Gly Tyr Tyr Leu Thr Leu Val Lys Lys Asp Val Glu Ser Ser Leu
1125 1130 1135 agt tcc tgc aga aac agt agt agc act gtg tca tac ctg
aaa aag 3775Ser Ser Cys Arg Asn Ser Ser Ser Thr Val Ser Tyr Leu Lys
Lys 1140 1145 1150 gag gac agt gtt tct cag agc agt tct gat gct ggc
ctg ggc agc 3820Glu Asp Ser Val Ser Gln Ser Ser Ser Asp Ala Gly Leu
Gly Ser 1155 1160 1165 gac cat gag agt gac acg ctg acc atc gat gtc
tct gct atc tcc 3865Asp His Glu Ser Asp Thr Leu Thr Ile Asp Val Ser
Ala Ile Ser 1170 1175 1180
aac ctc atc agg aag cat gtg tct gaa gcc cgg ctg gtg gaa gac 3910Asn
Leu Ile Arg Lys His Val Ser Glu Ala Arg Leu Val Glu Asp 1185 1190
1195 ata ggg cat gag ctg acc tat gtg ctg cca tat gaa gct gct aag
3955Ile Gly His Glu Leu Thr Tyr Val Leu Pro Tyr Glu Ala Ala Lys
1200 1205 1210 gag gga gcc ttt gtg gaa ctc ttt cat gag att gat gac
cgg ctc 4000Glu Gly Ala Phe Val Glu Leu Phe His Glu Ile Asp Asp Arg
Leu 1215 1220 1225 tca gac ctg ggc att tct agt tat ggc atc tca gag
acg acc ctg 4045Ser Asp Leu Gly Ile Ser Ser Tyr Gly Ile Ser Glu Thr
Thr Leu 1230 1235 1240 gaa gaa ata ttc ctc aag gtg gcc gaa gag agt
ggg gtg gat gct 4090Glu Glu Ile Phe Leu Lys Val Ala Glu Glu Ser Gly
Val Asp Ala 1245 1250 1255 gag acc tca gat ggt acc ttg cca gca aga
cga aac agg cgg gcc 4135Glu Thr Ser Asp Gly Thr Leu Pro Ala Arg Arg
Asn Arg Arg Ala 1260 1265 1270 ttc ggg gac aag cag agc tgt ctt cgc
ccg ttc act gaa gat gat 4180Phe Gly Asp Lys Gln Ser Cys Leu Arg Pro
Phe Thr Glu Asp Asp 1275 1280 1285 gct gct gat cca aat gat tct gac
ata gac cca gaa tcc aga gag 4225Ala Ala Asp Pro Asn Asp Ser Asp Ile
Asp Pro Glu Ser Arg Glu 1290 1295 1300 aca gac ttg ctc agt ggg atg
gat ggc aaa ggg tcc tac cag gtg 4270Thr Asp Leu Leu Ser Gly Met Asp
Gly Lys Gly Ser Tyr Gln Val 1305 1310 1315 aaa ggc tgg aaa ctt aca
cag caa cag ttt gtg gcc ctt ttg tgg 4315Lys Gly Trp Lys Leu Thr Gln
Gln Gln Phe Val Ala Leu Leu Trp 1320 1325 1330 aag aga ctg cta att
gcc aga cgg agt cgg aaa gga ttt ttt gct 4360Lys Arg Leu Leu Ile Ala
Arg Arg Ser Arg Lys Gly Phe Phe Ala 1335 1340 1345 cag att gtc ttg
cca gct gtg ttt gtc tgc att gcc ctt gtg ttc 4405Gln Ile Val Leu Pro
Ala Val Phe Val Cys Ile Ala Leu Val Phe 1350 1355 1360 agc ctg atc
gtg cca ccc ttt ggc aag tac ccc agc ctg gaa ctt 4450Ser Leu Ile Val
Pro Pro Phe Gly Lys Tyr Pro Ser Leu Glu Leu 1365 1370 1375 cag ccc
tgg atg tac aac gaa cag tac aca ttt gtc agc aat gat 4495Gln Pro Trp
Met Tyr Asn Glu Gln Tyr Thr Phe Val Ser Asn Asp 1380 1385 1390 gct
cct gag gac acg gga acc ctg gaa ctc tta aac gcc ctc acc 4540Ala Pro
Glu Asp Thr Gly Thr Leu Glu Leu Leu Asn Ala Leu Thr 1395 1400 1405
aaa gac cct ggc ttc ggg acc cgc tgt atg gaa gga aac cca atc 4585Lys
Asp Pro Gly Phe Gly Thr Arg Cys Met Glu Gly Asn Pro Ile 1410 1415
1420 cca gac acg ccc tgc cag gca ggg gag gaa gag tgg acc act gcc
4630Pro Asp Thr Pro Cys Gln Ala Gly Glu Glu Glu Trp Thr Thr Ala
1425 1430 1435 cca gtt ccc cag acc atc atg gac ctc ttc cag aat ggg
aac tgg 4675Pro Val Pro Gln Thr Ile Met Asp Leu Phe Gln Asn Gly Asn
Trp 1440 1445 1450 aca atg cag aac cct tca cct gca tgc cag tgt agc
agc gac aaa 4720Thr Met Gln Asn Pro Ser Pro Ala Cys Gln Cys Ser Ser
Asp Lys 1455 1460 1465 atc aag aag atg ctg cct gtg tgt ccc cca ggg
gca ggg ggg ctg 4765Ile Lys Lys Met Leu Pro Val Cys Pro Pro Gly Ala
Gly Gly Leu 1470 1475 1480 cct cct cca caa aga aaa caa aac act gca
gat atc ctt cag gac 4810Pro Pro Pro Gln Arg Lys Gln Asn Thr Ala Asp
Ile Leu Gln Asp 1485 1490 1495 ctg aca gga aga aac att tcg gat tat
ctg gtg aag acg tat gtg 4855Leu Thr Gly Arg Asn Ile Ser Asp Tyr Leu
Val Lys Thr Tyr Val 1500 1505 1510 cag atc ata gcc aaa agc tta aag
aac aag atc tgg gtg aat gag 4900Gln Ile Ile Ala Lys Ser Leu Lys Asn
Lys Ile Trp Val Asn Glu 1515 1520 1525 ttt agg tat ggc ggc ttt tcc
ctg ggt gtc agt aat act caa gca 4945Phe Arg Tyr Gly Gly Phe Ser Leu
Gly Val Ser Asn Thr Gln Ala 1530 1535 1540 ctt cct ccg agt caa gaa
gtt aat gat gcc atc aaa caa atg aag 4990Leu Pro Pro Ser Gln Glu Val
Asn Asp Ala Ile Lys Gln Met Lys 1545 1550 1555 aaa cac cta aag ctg
gcc aag gac agt tct gca gat cga ttt ctc 5035Lys His Leu Lys Leu Ala
Lys Asp Ser Ser Ala Asp Arg Phe Leu 1560 1565 1570 aac agc ttg gga
aga ttt atg aca gga ctg gac acc aaa aat aat 5080Asn Ser Leu Gly Arg
Phe Met Thr Gly Leu Asp Thr Lys Asn Asn 1575 1580 1585 gtc aag gtg
tgg ttc aat aac aag ggc tgg cat gca atc agc tct 5125Val Lys Val Trp
Phe Asn Asn Lys Gly Trp His Ala Ile Ser Ser 1590 1595 1600 ttc ctg
aat gtc atc aac aat gcc att ctc cgg gcc aac ctg caa 5170Phe Leu Asn
Val Ile Asn Asn Ala Ile Leu Arg Ala Asn Leu Gln 1605 1610 1615 aag
gga gag aac cct agc cat tat gga att act gct ttc aat cat 5215Lys Gly
Glu Asn Pro Ser His Tyr Gly Ile Thr Ala Phe Asn His 1620 1625 1630
ccc ctg aat ctc acc aag cag cag ctc tca gag gtg gct ctg atg 5260Pro
Leu Asn Leu Thr Lys Gln Gln Leu Ser Glu Val Ala Leu Met 1635 1640
1645 acc aca tca gtg gat gtc ctt gtg tcc atc tgt gtc atc ttt gca
5305Thr Thr Ser Val Asp Val Leu Val Ser Ile Cys Val Ile Phe Ala
1650 1655 1660 atg tcc ttc gtc cca gcc agc ttt gtc gta ttc ctg atc
cag gag 5350Met Ser Phe Val Pro Ala Ser Phe Val Val Phe Leu Ile Gln
Glu 1665 1670 1675 cgg gtc agc aaa gca aaa cac ctg cag ttc atc agt
gga gtg aag 5395Arg Val Ser Lys Ala Lys His Leu Gln Phe Ile Ser Gly
Val Lys 1680 1685 1690 cct gtc atc tac tgg ctc tct aat ttt gtc tgg
gat atg tgc aat 5440Pro Val Ile Tyr Trp Leu Ser Asn Phe Val Trp Asp
Met Cys Asn 1695 1700 1705 tac gtt gtc cct gcc aca ctg gtc att atc
atc ttc atc tgc ttc 5485Tyr Val Val Pro Ala Thr Leu Val Ile Ile Ile
Phe Ile Cys Phe 1710 1715 1720 cag cag aag tcc tat gtg tcc tcc acc
aat ctg cct gtg cta gcc 5530Gln Gln Lys Ser Tyr Val Ser Ser Thr Asn
Leu Pro Val Leu Ala 1725 1730 1735 ctt cta ctt ttg ctg tat ggg tgg
tca atc aca cct ctc atg tac 5575Leu Leu Leu Leu Leu Tyr Gly Trp Ser
Ile Thr Pro Leu Met Tyr 1740 1745 1750 cca gcc tcc ttt gtg ttc aag
atc ccc agc aca gcc tat gtg gtg 5620Pro Ala Ser Phe Val Phe Lys Ile
Pro Ser Thr Ala Tyr Val Val 1755 1760 1765 ctc acc agc gtg aac ctc
ttc att ggc att aat ggc agc gtg gcc 5665Leu Thr Ser Val Asn Leu Phe
Ile Gly Ile Asn Gly Ser Val Ala 1770 1775 1780 acc ttt gtg ctg gag
ctg ttc acc gac aat aag ctg aat aat atc 5710Thr Phe Val Leu Glu Leu
Phe Thr Asp Asn Lys Leu Asn Asn Ile 1785 1790 1795 aat gat atc ctg
aag tcc gtg ttc ttg atc ttc cca cat ttt tgc 5755Asn Asp Ile Leu Lys
Ser Val Phe Leu Ile Phe Pro His Phe Cys 1800 1805 1810 ctg gga cga
ggg ctc atc gac atg gtg aaa aac cag gca atg gct 5800Leu Gly Arg Gly
Leu Ile Asp Met Val Lys Asn Gln Ala Met Ala 1815 1820 1825 gat gcc
ctg gaa agg ttt ggg gag aat cgc ttt gtg tca cca tta 5845Asp Ala Leu
Glu Arg Phe Gly Glu Asn Arg Phe Val Ser Pro Leu 1830 1835 1840 tct
tgg gac ttg gtg gga cga aac ctc ttc gcc atg gcc gtg gaa 5890Ser Trp
Asp Leu Val Gly Arg Asn Leu Phe Ala Met Ala Val Glu 1845 1850 1855
ggg gtg gtg ttc ttc ctc att act gtt ctg atc cag tac aga ttc 5935Gly
Val Val Phe Phe Leu Ile Thr Val Leu Ile Gln Tyr Arg Phe 1860 1865
1870 ttc atc agg ccc aga cct gta aat gca aag cta tct cct ctg aat
5980Phe Ile Arg Pro Arg Pro Val Asn Ala Lys Leu Ser Pro Leu Asn
1875 1880 1885 gat gaa gat gaa gat gtg agg cgg gaa aga cag aga att
ctt gat 6025Asp Glu Asp Glu Asp Val Arg Arg Glu Arg Gln Arg Ile Leu
Asp 1890 1895 1900 ggt gga ggc cag aat gac atc tta gaa atc aag gag
ttg acg aag 6070Gly Gly Gly Gln Asn Asp Ile Leu Glu Ile Lys Glu Leu
Thr Lys 1905 1910 1915 ata tat aga agg aag cgg aag cct gct gtt gac
agg att tgc gtg 6115Ile Tyr Arg Arg Lys Arg Lys Pro Ala Val Asp Arg
Ile Cys Val 1920 1925 1930 ggc att cct cct ggt gag tgc ttt ggg ctc
ctg gga gtt aat ggg 6160Gly Ile Pro Pro Gly Glu Cys Phe Gly Leu Leu
Gly Val Asn Gly 1935 1940 1945 gct gga aaa tca tca act ttc aag atg
tta aca gga gat acc act 6205Ala Gly Lys Ser Ser Thr Phe Lys Met Leu
Thr Gly Asp Thr Thr 1950 1955 1960 gtt acc aga gga gat gct ttc ctt
aac aaa aat agt atc tta tca 6250Val Thr Arg Gly Asp Ala Phe Leu Asn
Lys Asn Ser Ile Leu Ser 1965 1970 1975 aac atc cat gaa gta cat cag
aac atg ggc tac tgc cct cag ttt 6295Asn Ile His Glu Val His Gln Asn
Met Gly Tyr Cys Pro Gln Phe 1980 1985 1990 gat gcc atc aca gag ctg
ttg act ggg aga gaa cac gtg gag ttc 6340Asp Ala Ile Thr Glu Leu Leu
Thr Gly Arg Glu His Val Glu Phe 1995 2000 2005 ttt gcc ctt ttg aga
gga gtc cca gag aaa gaa gtt ggc aag gtt 6385Phe Ala Leu Leu Arg Gly
Val Pro Glu Lys Glu Val Gly Lys Val 2010 2015 2020 ggt gag tgg gcg
att cgg aaa ctg ggc ctc gtg aag tat gga gaa 6430Gly Glu Trp Ala Ile
Arg Lys Leu Gly Leu Val Lys Tyr Gly Glu 2025 2030 2035 aaa tat gct
ggt aac tat agt gga ggc aac aaa cgc aag ctc tct 6475Lys Tyr Ala Gly
Asn Tyr Ser Gly Gly Asn Lys Arg Lys Leu Ser 2040 2045 2050 aca gcc
atg gct ttg atc ggc ggg cct cct gtg gtg ttt ctg gat 6520Thr Ala Met
Ala Leu Ile Gly Gly Pro Pro Val Val Phe Leu Asp 2055 2060 2065 gaa
ccc acc aca ggc atg gat ccc aaa gcc cgg cgg ttc ttg tgg 6565Glu Pro
Thr Thr Gly Met Asp Pro Lys Ala Arg Arg Phe Leu Trp 2070 2075 2080
aat tgt gcc cta agt gtt gtc aag gag ggg aga tca gta gtg ctt 6610Asn
Cys Ala Leu Ser Val Val Lys Glu Gly Arg Ser Val Val Leu 2085 2090
2095 aca tct cat agt atg gaa gaa tgt gaa gct ctt tgc act agg atg
6655Thr Ser His Ser Met Glu Glu Cys Glu Ala Leu Cys Thr Arg Met
2100 2105 2110 gca atc atg gtc aat gga agg ttc agg tgc ctt ggc agt
gtc cag 6700Ala Ile Met Val Asn Gly Arg Phe Arg Cys Leu Gly Ser Val
Gln 2115 2120 2125 cat cta aaa aat agg ttt gga gat ggt tat aca ata
gtt gta cga 6745His Leu Lys Asn Arg Phe Gly Asp Gly Tyr Thr Ile Val
Val Arg 2130 2135 2140 ata gca ggg tcc aac ccg gac ctg aag cct gtc
cag gat ttc ttt 6790Ile Ala Gly Ser Asn Pro Asp Leu Lys Pro Val Gln
Asp Phe Phe 2145 2150 2155 gga ctt gca ttt cct gga agt gtt cta aaa
gag aaa cac cgg aac 6835Gly Leu Ala Phe Pro Gly Ser Val Leu Lys Glu
Lys His Arg Asn 2160 2165 2170 atg cta caa tac cag ctt cca tct tca
tta tct tct ctg gcc agg 6880Met Leu Gln Tyr Gln Leu Pro Ser Ser Leu
Ser Ser Leu Ala Arg 2175 2180 2185 ata ttc agc atc ctc tcc cag agc
aaa aag cga ctc cac ata gaa 6925Ile Phe Ser Ile Leu Ser Gln Ser Lys
Lys Arg Leu His Ile Glu 2190 2195 2200 gac tac tct gtt tct cag aca
aca ctt gac caa gta ttt gtg aac 6970Asp Tyr Ser Val Ser Gln Thr Thr
Leu Asp Gln Val Phe Val Asn 2205 2210 2215 ttt gcc aag gac caa agt
gat gat gac cac tta aaa gac ctc tca 7015Phe Ala Lys Asp Gln Ser Asp
Asp Asp His Leu Lys Asp Leu Ser 2220 2225 2230 tta cac aaa aac cag
aca gta gtg gac gtt gca gtt ctc aca tct 7060Leu His Lys Asn Gln Thr
Val Val Asp Val Ala Val Leu Thr Ser 2235 2240 2245 ttt cta cag gat
gag aaa gtg aaa gaa agc tat gta tga agaatcctgt 7109Phe Leu Gln Asp
Glu Lys Val Lys Glu Ser Tyr Val 2250 2255 2260 tcatacgggg
tggctgaaag taaagaggaa c 714072261PRTHomo sapiens 7Met Ala Cys Trp
Pro Gln Leu Arg Leu Leu Leu Trp Lys Asn Leu Thr 1 5 10 15 Phe Arg
Arg Arg Gln Thr Cys Gln Leu Leu Leu Glu Val Ala Trp Pro 20 25 30
Leu Phe Ile Phe Leu Ile Leu Ile Ser Val Arg Leu Ser Tyr Pro Pro 35
40 45 Tyr Glu Gln His Glu Cys His Phe Pro Asn Lys Ala Met Pro Ser
Ala 50 55 60 Gly Thr Leu Pro Trp Val Gln Gly Ile Ile Cys Asn Ala
Asn Asn Pro 65 70 75 80 Cys Phe Arg Tyr Pro Thr Pro Gly Glu Ala Pro
Gly Val Val Gly Asn 85 90 95 Phe Asn Lys Ser Ile Val Ala Arg Leu
Phe Ser Asp Ala Arg Arg Leu 100 105 110 Leu Leu Tyr Ser Gln Lys Asp
Thr Ser Met Lys Asp Met Arg Lys Val 115 120 125 Leu Arg Thr Leu Gln
Gln Ile Lys Lys Ser Ser Ser Asn Leu Lys Leu 130 135 140 Gln Asp Phe
Leu Val Asp Asn Glu Thr Phe Ser Gly Phe Leu Tyr His 145 150 155 160
Asn Leu Ser Leu Pro Lys Ser Thr Val Asp Lys Met Leu Arg Ala Asp 165
170 175 Val Ile Leu His Lys Val Phe Leu Gln Gly Tyr Gln Leu His Leu
Thr 180 185 190 Ser Leu Cys Asn Gly Ser Lys Ser Glu Glu Met Ile Gln
Leu Gly Asp 195 200 205 Gln Glu Val Ser Glu Leu Cys Gly Leu Pro Arg
Glu Lys Leu Ala Ala 210 215 220 Ala Glu Arg Val Leu Arg Ser Asn Met
Asp Ile Leu Lys Pro Ile Leu 225 230 235 240 Arg Thr Leu Asn Ser Thr
Ser Pro Phe Pro Ser Lys Glu Leu Ala Glu 245 250 255 Ala Thr Lys Thr
Leu Leu His Ser Leu Gly Thr Leu Ala Gln Glu Leu 260 265 270 Phe Ser
Met Arg Ser Trp Ser Asp Met Arg Gln Glu Val Met Phe Leu 275 280 285
Thr Asn Val Asn Ser Ser Ser Ser Ser Thr Gln Ile Tyr Gln Ala Val 290
295 300 Ser Arg Ile Val Cys Gly His Pro Glu Gly Gly Gly
Leu Lys Ile Lys 305 310 315 320 Ser Leu Asn Trp Tyr Glu Asp Asn Asn
Tyr Lys Ala Leu Phe Gly Gly 325 330 335 Asn Gly Thr Glu Glu Asp Ala
Glu Thr Phe Tyr Asp Asn Ser Thr Thr 340 345 350 Pro Tyr Cys Asn Asp
Leu Met Lys Asn Leu Glu Ser Ser Pro Leu Ser 355 360 365 Arg Ile Ile
Trp Lys Ala Leu Lys Pro Leu Leu Val Gly Lys Ile Leu 370 375 380 Tyr
Thr Pro Asp Thr Pro Ala Thr Arg Gln Val Met Ala Glu Val Asn 385 390
395 400 Lys Thr Phe Gln Glu Leu Ala Val Phe His Asp Leu Glu Gly Met
Trp 405 410 415 Glu Glu Leu Ser Pro Lys Ile Trp Thr Phe Met Glu Asn
Ser Gln Glu 420 425 430 Met Asp Leu Val Arg Met Leu Leu Asp Ser Arg
Asp Asn Asp His Phe 435 440 445 Trp Glu Gln Gln Leu Asp Gly Leu Asp
Trp Thr Ala Gln Asp Ile Val 450 455 460 Ala Phe Leu Ala Lys His Pro
Glu Asp Val Gln Ser Ser Asn Gly Ser 465 470 475 480 Val Tyr Thr Trp
Arg Glu Ala Phe Asn Glu Thr Asn Gln Ala Ile Arg 485 490 495 Thr Ile
Ser Arg Phe Met Glu Cys Val Asn Leu Asn Lys Leu Glu Pro 500 505 510
Ile Ala Thr Glu Val Trp Leu Ile Asn Lys Ser Met Glu Leu Leu Asp 515
520 525 Glu Arg Lys Phe Trp Ala Gly Ile Val Phe Thr Gly Ile Thr Pro
Gly 530 535 540 Ser Ile Glu Leu Pro His His Val Lys Tyr Lys Ile Arg
Met Asp Ile 545 550 555 560 Asp Asn Val Glu Arg Thr Asn Lys Ile Lys
Asp Gly Tyr Trp Asp Pro 565 570 575 Gly Pro Arg Ala Asp Pro Phe Glu
Asp Met Arg Tyr Val Trp Gly Gly 580 585 590 Phe Ala Tyr Leu Gln Asp
Val Val Glu Gln Ala Ile Ile Arg Val Leu 595 600 605 Thr Gly Thr Glu
Lys Lys Thr Gly Val Tyr Met Gln Gln Met Pro Tyr 610 615 620 Pro Cys
Tyr Val Asp Asp Ile Phe Leu Arg Val Met Ser Arg Ser Met 625 630 635
640 Pro Leu Phe Met Thr Leu Ala Trp Ile Tyr Ser Val Ala Val Ile Ile
645 650 655 Lys Gly Ile Val Tyr Glu Lys Glu Ala Arg Leu Lys Glu Thr
Met Arg 660 665 670 Ile Met Gly Leu Asp Asn Ser Ile Leu Trp Phe Ser
Trp Phe Ile Ser 675 680 685 Ser Leu Ile Pro Leu Leu Val Ser Ala Gly
Leu Leu Val Val Ile Leu 690 695 700 Lys Leu Gly Asn Leu Leu Pro Tyr
Ser Asp Pro Ser Val Val Phe Val 705 710 715 720 Phe Leu Ser Val Phe
Ala Val Val Thr Ile Leu Gln Cys Phe Leu Ile 725 730 735 Ser Thr Leu
Phe Ser Arg Ala Asn Leu Ala Ala Ala Cys Gly Gly Ile 740 745 750 Ile
Tyr Phe Thr Leu Tyr Leu Pro Tyr Val Leu Cys Val Ala Trp Gln 755 760
765 Asp Tyr Val Gly Phe Thr Leu Lys Ile Phe Ala Ser Leu Leu Ser Pro
770 775 780 Val Ala Phe Gly Phe Gly Cys Glu Tyr Phe Ala Leu Phe Glu
Glu Gln 785 790 795 800 Gly Ile Gly Val Gln Trp Asp Asn Leu Phe Glu
Ser Pro Val Glu Glu 805 810 815 Asp Gly Phe Asn Leu Thr Thr Ser Val
Ser Met Met Leu Phe Asp Thr 820 825 830 Phe Leu Tyr Gly Val Met Thr
Trp Tyr Ile Glu Ala Val Phe Pro Gly 835 840 845 Gln Tyr Gly Ile Pro
Arg Pro Trp Tyr Phe Pro Cys Thr Lys Ser Tyr 850 855 860 Trp Phe Gly
Glu Glu Ser Asp Glu Lys Ser His Pro Gly Ser Asn Gln 865 870 875 880
Lys Arg Ile Ser Glu Ile Cys Met Glu Glu Glu Pro Thr His Leu Lys 885
890 895 Leu Gly Val Ser Ile Gln Asn Leu Val Lys Val Tyr Arg Asp Gly
Met 900 905 910 Lys Val Ala Val Asp Gly Leu Ala Leu Asn Phe Tyr Glu
Gly Gln Ile 915 920 925 Thr Ser Phe Leu Gly His Asn Gly Ala Gly Lys
Thr Thr Thr Met Ser 930 935 940 Ile Leu Thr Gly Leu Phe Pro Pro Thr
Ser Gly Thr Ala Tyr Ile Leu 945 950 955 960 Gly Lys Asp Ile Arg Ser
Glu Met Ser Thr Ile Arg Gln Asn Leu Gly 965 970 975 Val Cys Pro Gln
His Asn Val Leu Phe Asp Met Leu Thr Val Glu Glu 980 985 990 His Ile
Trp Phe Tyr Ala Arg Leu Lys Gly Leu Ser Glu Lys His Val 995 1000
1005 Lys Ala Glu Met Glu Gln Met Ala Leu Asp Val Gly Leu Pro Ser
1010 1015 1020 Ser Lys Leu Lys Ser Lys Thr Ser Gln Leu Ser Gly Gly
Met Gln 1025 1030 1035 Arg Lys Leu Ser Val Ala Leu Ala Phe Val Gly
Gly Ser Lys Val 1040 1045 1050 Val Ile Leu Asp Glu Pro Thr Ala Gly
Val Asp Pro Tyr Ser Arg 1055 1060 1065 Arg Gly Ile Trp Glu Leu Leu
Leu Lys Tyr Arg Gln Gly Arg Thr 1070 1075 1080 Ile Ile Leu Ser Thr
His His Met Asp Glu Ala Asp Val Leu Gly 1085 1090 1095 Asp Arg Ile
Ala Ile Ile Ser His Gly Lys Leu Cys Cys Val Gly 1100 1105 1110 Ser
Ser Leu Phe Leu Lys Asn Gln Leu Gly Thr Gly Tyr Tyr Leu 1115 1120
1125 Thr Leu Val Lys Lys Asp Val Glu Ser Ser Leu Ser Ser Cys Arg
1130 1135 1140 Asn Ser Ser Ser Thr Val Ser Tyr Leu Lys Lys Glu Asp
Ser Val 1145 1150 1155 Ser Gln Ser Ser Ser Asp Ala Gly Leu Gly Ser
Asp His Glu Ser 1160 1165 1170 Asp Thr Leu Thr Ile Asp Val Ser Ala
Ile Ser Asn Leu Ile Arg 1175 1180 1185 Lys His Val Ser Glu Ala Arg
Leu Val Glu Asp Ile Gly His Glu 1190 1195 1200 Leu Thr Tyr Val Leu
Pro Tyr Glu Ala Ala Lys Glu Gly Ala Phe 1205 1210 1215 Val Glu Leu
Phe His Glu Ile Asp Asp Arg Leu Ser Asp Leu Gly 1220 1225 1230 Ile
Ser Ser Tyr Gly Ile Ser Glu Thr Thr Leu Glu Glu Ile Phe 1235 1240
1245 Leu Lys Val Ala Glu Glu Ser Gly Val Asp Ala Glu Thr Ser Asp
1250 1255 1260 Gly Thr Leu Pro Ala Arg Arg Asn Arg Arg Ala Phe Gly
Asp Lys 1265 1270 1275 Gln Ser Cys Leu Arg Pro Phe Thr Glu Asp Asp
Ala Ala Asp Pro 1280 1285 1290 Asn Asp Ser Asp Ile Asp Pro Glu Ser
Arg Glu Thr Asp Leu Leu 1295 1300 1305 Ser Gly Met Asp Gly Lys Gly
Ser Tyr Gln Val Lys Gly Trp Lys 1310 1315 1320 Leu Thr Gln Gln Gln
Phe Val Ala Leu Leu Trp Lys Arg Leu Leu 1325 1330 1335 Ile Ala Arg
Arg Ser Arg Lys Gly Phe Phe Ala Gln Ile Val Leu 1340 1345 1350 Pro
Ala Val Phe Val Cys Ile Ala Leu Val Phe Ser Leu Ile Val 1355 1360
1365 Pro Pro Phe Gly Lys Tyr Pro Ser Leu Glu Leu Gln Pro Trp Met
1370 1375 1380 Tyr Asn Glu Gln Tyr Thr Phe Val Ser Asn Asp Ala Pro
Glu Asp 1385 1390 1395 Thr Gly Thr Leu Glu Leu Leu Asn Ala Leu Thr
Lys Asp Pro Gly 1400 1405 1410 Phe Gly Thr Arg Cys Met Glu Gly Asn
Pro Ile Pro Asp Thr Pro 1415 1420 1425 Cys Gln Ala Gly Glu Glu Glu
Trp Thr Thr Ala Pro Val Pro Gln 1430 1435 1440 Thr Ile Met Asp Leu
Phe Gln Asn Gly Asn Trp Thr Met Gln Asn 1445 1450 1455 Pro Ser Pro
Ala Cys Gln Cys Ser Ser Asp Lys Ile Lys Lys Met 1460 1465 1470 Leu
Pro Val Cys Pro Pro Gly Ala Gly Gly Leu Pro Pro Pro Gln 1475 1480
1485 Arg Lys Gln Asn Thr Ala Asp Ile Leu Gln Asp Leu Thr Gly Arg
1490 1495 1500 Asn Ile Ser Asp Tyr Leu Val Lys Thr Tyr Val Gln Ile
Ile Ala 1505 1510 1515 Lys Ser Leu Lys Asn Lys Ile Trp Val Asn Glu
Phe Arg Tyr Gly 1520 1525 1530 Gly Phe Ser Leu Gly Val Ser Asn Thr
Gln Ala Leu Pro Pro Ser 1535 1540 1545 Gln Glu Val Asn Asp Ala Ile
Lys Gln Met Lys Lys His Leu Lys 1550 1555 1560 Leu Ala Lys Asp Ser
Ser Ala Asp Arg Phe Leu Asn Ser Leu Gly 1565 1570 1575 Arg Phe Met
Thr Gly Leu Asp Thr Lys Asn Asn Val Lys Val Trp 1580 1585 1590 Phe
Asn Asn Lys Gly Trp His Ala Ile Ser Ser Phe Leu Asn Val 1595 1600
1605 Ile Asn Asn Ala Ile Leu Arg Ala Asn Leu Gln Lys Gly Glu Asn
1610 1615 1620 Pro Ser His Tyr Gly Ile Thr Ala Phe Asn His Pro Leu
Asn Leu 1625 1630 1635 Thr Lys Gln Gln Leu Ser Glu Val Ala Leu Met
Thr Thr Ser Val 1640 1645 1650 Asp Val Leu Val Ser Ile Cys Val Ile
Phe Ala Met Ser Phe Val 1655 1660 1665 Pro Ala Ser Phe Val Val Phe
Leu Ile Gln Glu Arg Val Ser Lys 1670 1675 1680 Ala Lys His Leu Gln
Phe Ile Ser Gly Val Lys Pro Val Ile Tyr 1685 1690 1695 Trp Leu Ser
Asn Phe Val Trp Asp Met Cys Asn Tyr Val Val Pro 1700 1705 1710 Ala
Thr Leu Val Ile Ile Ile Phe Ile Cys Phe Gln Gln Lys Ser 1715 1720
1725 Tyr Val Ser Ser Thr Asn Leu Pro Val Leu Ala Leu Leu Leu Leu
1730 1735 1740 Leu Tyr Gly Trp Ser Ile Thr Pro Leu Met Tyr Pro Ala
Ser Phe 1745 1750 1755 Val Phe Lys Ile Pro Ser Thr Ala Tyr Val Val
Leu Thr Ser Val 1760 1765 1770 Asn Leu Phe Ile Gly Ile Asn Gly Ser
Val Ala Thr Phe Val Leu 1775 1780 1785 Glu Leu Phe Thr Asp Asn Lys
Leu Asn Asn Ile Asn Asp Ile Leu 1790 1795 1800 Lys Ser Val Phe Leu
Ile Phe Pro His Phe Cys Leu Gly Arg Gly 1805 1810 1815 Leu Ile Asp
Met Val Lys Asn Gln Ala Met Ala Asp Ala Leu Glu 1820 1825 1830 Arg
Phe Gly Glu Asn Arg Phe Val Ser Pro Leu Ser Trp Asp Leu 1835 1840
1845 Val Gly Arg Asn Leu Phe Ala Met Ala Val Glu Gly Val Val Phe
1850 1855 1860 Phe Leu Ile Thr Val Leu Ile Gln Tyr Arg Phe Phe Ile
Arg Pro 1865 1870 1875 Arg Pro Val Asn Ala Lys Leu Ser Pro Leu Asn
Asp Glu Asp Glu 1880 1885 1890 Asp Val Arg Arg Glu Arg Gln Arg Ile
Leu Asp Gly Gly Gly Gln 1895 1900 1905 Asn Asp Ile Leu Glu Ile Lys
Glu Leu Thr Lys Ile Tyr Arg Arg 1910 1915 1920 Lys Arg Lys Pro Ala
Val Asp Arg Ile Cys Val Gly Ile Pro Pro 1925 1930 1935 Gly Glu Cys
Phe Gly Leu Leu Gly Val Asn Gly Ala Gly Lys Ser 1940 1945 1950 Ser
Thr Phe Lys Met Leu Thr Gly Asp Thr Thr Val Thr Arg Gly 1955 1960
1965 Asp Ala Phe Leu Asn Lys Asn Ser Ile Leu Ser Asn Ile His Glu
1970 1975 1980 Val His Gln Asn Met Gly Tyr Cys Pro Gln Phe Asp Ala
Ile Thr 1985 1990 1995 Glu Leu Leu Thr Gly Arg Glu His Val Glu Phe
Phe Ala Leu Leu 2000 2005 2010 Arg Gly Val Pro Glu Lys Glu Val Gly
Lys Val Gly Glu Trp Ala 2015 2020 2025 Ile Arg Lys Leu Gly Leu Val
Lys Tyr Gly Glu Lys Tyr Ala Gly 2030 2035 2040 Asn Tyr Ser Gly Gly
Asn Lys Arg Lys Leu Ser Thr Ala Met Ala 2045 2050 2055 Leu Ile Gly
Gly Pro Pro Val Val Phe Leu Asp Glu Pro Thr Thr 2060 2065 2070 Gly
Met Asp Pro Lys Ala Arg Arg Phe Leu Trp Asn Cys Ala Leu 2075 2080
2085 Ser Val Val Lys Glu Gly Arg Ser Val Val Leu Thr Ser His Ser
2090 2095 2100 Met Glu Glu Cys Glu Ala Leu Cys Thr Arg Met Ala Ile
Met Val 2105 2110 2115 Asn Gly Arg Phe Arg Cys Leu Gly Ser Val Gln
His Leu Lys Asn 2120 2125 2130 Arg Phe Gly Asp Gly Tyr Thr Ile Val
Val Arg Ile Ala Gly Ser 2135 2140 2145 Asn Pro Asp Leu Lys Pro Val
Gln Asp Phe Phe Gly Leu Ala Phe 2150 2155 2160 Pro Gly Ser Val Leu
Lys Glu Lys His Arg Asn Met Leu Gln Tyr 2165 2170 2175 Gln Leu Pro
Ser Ser Leu Ser Ser Leu Ala Arg Ile Phe Ser Ile 2180 2185 2190 Leu
Ser Gln Ser Lys Lys Arg Leu His Ile Glu Asp Tyr Ser Val 2195 2200
2205 Ser Gln Thr Thr Leu Asp Gln Val Phe Val Asn Phe Ala Lys Asp
2210 2215 2220 Gln Ser Asp Asp Asp His Leu Lys Asp Leu Ser Leu His
Lys Asn 2225 2230 2235 Gln Thr Val Val Asp Val Ala Val Leu Thr Ser
Phe Leu Gln Asp 2240 2245 2250 Glu Lys Val Lys Glu Ser Tyr Val 2255
2260 8780DNARattus norvegicusCDS(63)..(731) 8gcagctttaa gtagagagtg
gatttttgtc tcagtttgtc ttctgtttgc gactctgaaa 60ga atg ctg tgg gca
ctc ttt ttc ctg gtg act act att cac gct gaa 107 Met Leu Trp Ala Leu
Phe Phe Leu Val Thr Thr Ile His Ala Glu 1 5 10 15 ctc tgc cgt cca
gat gca gaa aat gcc ttt aaa gta aga ctt agc atc 155Leu Cys Arg Pro
Asp Ala Glu Asn Ala Phe Lys Val Arg Leu Ser Ile 20 25 30 aaa gca
gct ctt gga gat aaa gcg tat gtc tgg gac aca gat gaa gaa 203Lys Ala
Ala Leu Gly Asp Lys Ala Tyr Val Trp Asp Thr Asp Glu Glu 35 40 45
tat ctc ttc aga gca atg gtg gca ttc tcc atg aga aaa gtt ccc aac
251Tyr Leu Phe Arg Ala Met Val Ala Phe Ser Met Arg Lys Val Pro Asn
50 55 60 aga gaa gga aca gaa att tcc cac gtc ctg ctt tgc aat gta
acc cag 299Arg Glu Gly Thr Glu Ile Ser His Val Leu Leu Cys Asn Val
Thr Gln 65 70 75 aga gtg tca ttc tgg ttt gtg gtc aca gac cct ttg
aaa aac cat act 347Arg Val Ser Phe Trp Phe Val Val Thr Asp Pro Leu
Lys Asn His Thr 80 85 90 95 ctt cct gca gct gaa gta cag tca gcc ata
aga atg aac agg aac cgg 395Leu Pro Ala Ala Glu Val Gln Ser Ala Ile
Arg Met Asn Arg Asn Arg 100 105 110 atc aac agt gca ttc ttt ttg gat
gat cat act ctg gaa ttt tta aaa 443Ile Asn Ser Ala Phe Phe Leu Asp
Asp His Thr Leu Glu Phe Leu Lys 115 120 125 att cct tcc act ctt gct
ccc ccg atg gat cca tct gtg ccc gtc tgg 491Ile Pro Ser Thr Leu Ala
Pro Pro Met Asp Pro Ser Val Pro Val Trp 130 135 140 att att gta ttt
ggt gtg ata ttt tgc att gtt aca gtt gca att gca 539Ile Ile Val Phe
Gly Val Ile Phe Cys Ile Val Thr Val Ala Ile Ala 145 150 155 cta ctg
gtt tta tcc gga atc cgg caa cga
aga agg aac aag aaa gga 587Leu Leu Val Leu Ser Gly Ile Arg Gln Arg
Arg Arg Asn Lys Lys Gly 160 165 170 175 cca cct gga gtg gag gat gca
gaa gac aag tgt gaa aac atc atc aca 635Pro Pro Gly Val Glu Asp Ala
Glu Asp Lys Cys Glu Asn Ile Ile Thr 180 185 190 att gaa aat ggc atc
cct tgt gat ccc ttg gac atg aag gga ggg cac 683Ile Glu Asn Gly Ile
Pro Cys Asp Pro Leu Asp Met Lys Gly Gly His 195 200 205 att aat gat
ggc ttc ttg aca gag gat gag cgt ctc acc cct ctc tga 731Ile Asn Asp
Gly Phe Leu Thr Glu Asp Glu Arg Leu Thr Pro Leu 210 215 220
gagttacagt cttgtaagaa aatttcaaga tgcttgaatg tgatagaca
7809222PRTRattus norvegicus 9Met Leu Trp Ala Leu Phe Phe Leu Val
Thr Thr Ile His Ala Glu Leu 1 5 10 15 Cys Arg Pro Asp Ala Glu Asn
Ala Phe Lys Val Arg Leu Ser Ile Lys 20 25 30 Ala Ala Leu Gly Asp
Lys Ala Tyr Val Trp Asp Thr Asp Glu Glu Tyr 35 40 45 Leu Phe Arg
Ala Met Val Ala Phe Ser Met Arg Lys Val Pro Asn Arg 50 55 60 Glu
Gly Thr Glu Ile Ser His Val Leu Leu Cys Asn Val Thr Gln Arg 65 70
75 80 Val Ser Phe Trp Phe Val Val Thr Asp Pro Leu Lys Asn His Thr
Leu 85 90 95 Pro Ala Ala Glu Val Gln Ser Ala Ile Arg Met Asn Arg
Asn Arg Ile 100 105 110 Asn Ser Ala Phe Phe Leu Asp Asp His Thr Leu
Glu Phe Leu Lys Ile 115 120 125 Pro Ser Thr Leu Ala Pro Pro Met Asp
Pro Ser Val Pro Val Trp Ile 130 135 140 Ile Val Phe Gly Val Ile Phe
Cys Ile Val Thr Val Ala Ile Ala Leu 145 150 155 160 Leu Val Leu Ser
Gly Ile Arg Gln Arg Arg Arg Asn Lys Lys Gly Pro 165 170 175 Pro Gly
Val Glu Asp Ala Glu Asp Lys Cys Glu Asn Ile Ile Thr Ile 180 185 190
Glu Asn Gly Ile Pro Cys Asp Pro Leu Asp Met Lys Gly Gly His Ile 195
200 205 Asn Asp Gly Phe Leu Thr Glu Asp Glu Arg Leu Thr Pro Leu 210
215 220 101116DNAPlasmodium falciparumCDS(1)..(1116) 10atg cat ata
gtg agc ttt att att ttt ttc ttt gca tta ttt ttt cca 48Met His Ile
Val Ser Phe Ile Ile Phe Phe Phe Ala Leu Phe Phe Pro 1 5 10 15 att
tcc atc tgt tat aaa ata aat ggg gta tgt gat ttt tcg agc gaa 96Ile
Ser Ile Cys Tyr Lys Ile Asn Gly Val Cys Asp Phe Ser Ser Glu 20 25
30 ggg cta agt ttg ttg cca gaa gaa aag tta gat ttt tct gta tca agg
144Gly Leu Ser Leu Leu Pro Glu Glu Lys Leu Asp Phe Ser Val Ser Arg
35 40 45 aat gta gat aaa tta tct gat gaa aac aat gta aga cat tgt
gta cat 192Asn Val Asp Lys Leu Ser Asp Glu Asn Asn Val Arg His Cys
Val His 50 55 60 ttt agt aag ggt ttt gaa tat tta cgt ttt ata tgt
cca atg aga aaa 240Phe Ser Lys Gly Phe Glu Tyr Leu Arg Phe Ile Cys
Pro Met Arg Lys 65 70 75 80 gat aat tat gaa gga att gaa att cgt cct
gtt gaa tgt ttt gaa tat 288Asp Asn Tyr Glu Gly Ile Glu Ile Arg Pro
Val Glu Cys Phe Glu Tyr 85 90 95 att cat att gaa gga aga gaa cac
aaa tta agc gag ata tta aaa ggt 336Ile His Ile Glu Gly Arg Glu His
Lys Leu Ser Glu Ile Leu Lys Gly 100 105 110 agt tta tat gaa aaa agt
ata aat gat aat ata atg acg aga gat gtt 384Ser Leu Tyr Glu Lys Ser
Ile Asn Asp Asn Ile Met Thr Arg Asp Val 115 120 125 ttt att cct cca
act att tat gaa gat atg ttt ttt gaa tgt aca tgt 432Phe Ile Pro Pro
Thr Ile Tyr Glu Asp Met Phe Phe Glu Cys Thr Cys 130 135 140 gat aat
agt tta acc ttt aaa aat aat atg att ggt ata aga ggt ata 480Asp Asn
Ser Leu Thr Phe Lys Asn Asn Met Ile Gly Ile Arg Gly Ile 145 150 155
160 atg aaa atc cat tta aaa aaa aat att tta tat gga tgt gat ttt gat
528Met Lys Ile His Leu Lys Lys Asn Ile Leu Tyr Gly Cys Asp Phe Asp
165 170 175 cat gat gaa aaa tta atg aaa aat aaa aca gca ttt aca aat
ttt tat 576His Asp Glu Lys Leu Met Lys Asn Lys Thr Ala Phe Thr Asn
Phe Tyr 180 185 190 gat aaa caa aaa att tta cca tta ata ggt aat aat
aat aat gat gat 624Asp Lys Gln Lys Ile Leu Pro Leu Ile Gly Asn Asn
Asn Asn Asp Asp 195 200 205 gat aat aat gat gat gat aat aat aat gat
aat aat aat aat gat aat 672Asp Asn Asn Asp Asp Asp Asn Asn Asn Asp
Asn Asn Asn Asn Asp Asn 210 215 220 aat aat aat aat aat aat aat aat
aat aat aat aat aat aat aat aat 720Asn Asn Asn Asn Asn Asn Asn Asn
Asn Asn Asn Asn Asn Asn Asn Asn 225 230 235 240 aat aat att act tgt
aat gtt act att aaa aaa tct caa gtt tat tta 768Asn Asn Ile Thr Cys
Asn Val Thr Ile Lys Lys Ser Gln Val Tyr Leu 245 250 255 gga att ata
tgc cca gat gga tat act tta tat cca aat gat tgt ttt 816Gly Ile Ile
Cys Pro Asp Gly Tyr Thr Leu Tyr Pro Asn Asp Cys Phe 260 265 270 aaa
aat gtt ata tat gat aat aat att att ata cca tta aaa aaa att 864Lys
Asn Val Ile Tyr Asp Asn Asn Ile Ile Ile Pro Leu Lys Lys Ile 275 280
285 ata cca cat gat att tta tat cat caa gac aaa aac aaa aga att act
912Ile Pro His Asp Ile Leu Tyr His Gln Asp Lys Asn Lys Arg Ile Thr
290 295 300 ttt gct tca ttt aca tta aat ata aat gaa aat cca cca gga
ttc aca 960Phe Ala Ser Phe Thr Leu Asn Ile Asn Glu Asn Pro Pro Gly
Phe Thr 305 310 315 320 tgt tat tgt att aaa gat caa aca aat att aat
aac cca ctt atc gta 1008Cys Tyr Cys Ile Lys Asp Gln Thr Asn Ile Asn
Asn Pro Leu Ile Val 325 330 335 aac ttc cat ttt tca aat caa gaa aca
tca tat gca aca aaa aat aaa 1056Asn Phe His Phe Ser Asn Gln Glu Thr
Ser Tyr Ala Thr Lys Asn Lys 340 345 350 aat ctc ttc ttt tat ttt att
ttc atc ttc cct ttt ctt tat gtt att 1104Asn Leu Phe Phe Tyr Phe Ile
Phe Ile Phe Pro Phe Leu Tyr Val Ile 355 360 365 ttg tta tta taa
1116Leu Leu Leu 370 11371PRTPlasmodium falciparum 11Met His Ile Val
Ser Phe Ile Ile Phe Phe Phe Ala Leu Phe Phe Pro 1 5 10 15 Ile Ser
Ile Cys Tyr Lys Ile Asn Gly Val Cys Asp Phe Ser Ser Glu 20 25 30
Gly Leu Ser Leu Leu Pro Glu Glu Lys Leu Asp Phe Ser Val Ser Arg 35
40 45 Asn Val Asp Lys Leu Ser Asp Glu Asn Asn Val Arg His Cys Val
His 50 55 60 Phe Ser Lys Gly Phe Glu Tyr Leu Arg Phe Ile Cys Pro
Met Arg Lys 65 70 75 80 Asp Asn Tyr Glu Gly Ile Glu Ile Arg Pro Val
Glu Cys Phe Glu Tyr 85 90 95 Ile His Ile Glu Gly Arg Glu His Lys
Leu Ser Glu Ile Leu Lys Gly 100 105 110 Ser Leu Tyr Glu Lys Ser Ile
Asn Asp Asn Ile Met Thr Arg Asp Val 115 120 125 Phe Ile Pro Pro Thr
Ile Tyr Glu Asp Met Phe Phe Glu Cys Thr Cys 130 135 140 Asp Asn Ser
Leu Thr Phe Lys Asn Asn Met Ile Gly Ile Arg Gly Ile 145 150 155 160
Met Lys Ile His Leu Lys Lys Asn Ile Leu Tyr Gly Cys Asp Phe Asp 165
170 175 His Asp Glu Lys Leu Met Lys Asn Lys Thr Ala Phe Thr Asn Phe
Tyr 180 185 190 Asp Lys Gln Lys Ile Leu Pro Leu Ile Gly Asn Asn Asn
Asn Asp Asp 195 200 205 Asp Asn Asn Asp Asp Asp Asn Asn Asn Asp Asn
Asn Asn Asn Asp Asn 210 215 220 Asn Asn Asn Asn Asn Asn Asn Asn Asn
Asn Asn Asn Asn Asn Asn Asn 225 230 235 240 Asn Asn Ile Thr Cys Asn
Val Thr Ile Lys Lys Ser Gln Val Tyr Leu 245 250 255 Gly Ile Ile Cys
Pro Asp Gly Tyr Thr Leu Tyr Pro Asn Asp Cys Phe 260 265 270 Lys Asn
Val Ile Tyr Asp Asn Asn Ile Ile Ile Pro Leu Lys Lys Ile 275 280 285
Ile Pro His Asp Ile Leu Tyr His Gln Asp Lys Asn Lys Arg Ile Thr 290
295 300 Phe Ala Ser Phe Thr Leu Asn Ile Asn Glu Asn Pro Pro Gly Phe
Thr 305 310 315 320 Cys Tyr Cys Ile Lys Asp Gln Thr Asn Ile Asn Asn
Pro Leu Ile Val 325 330 335 Asn Phe His Phe Ser Asn Gln Glu Thr Ser
Tyr Ala Thr Lys Asn Lys 340 345 350 Asn Leu Phe Phe Tyr Phe Ile Phe
Ile Phe Pro Phe Leu Tyr Val Ile 355 360 365 Leu Leu Leu 370
1273PRTCricetulus migratorius 12Gln Arg Val Asp His His Phe Asn Glu
Pro Val Thr Ile Ala Ile Ile 1 5 10 15 Leu Gly Met Ile Ala Gly Ile
Val Gly Thr Ile Leu Leu Ile Tyr Tyr 20 25 30 Leu Ile Ser Leu Ile
Thr Lys Lys Ile Ser Ala Asp Lys Gln Pro Pro 35 40 45 Lys Ser Glu
Asn Thr Asp Glu Pro Pro Ser Pro Ile Glu Gln Ile Ile 50 55 60 Val
Gln Glu Glu His Asp Ser Ile Val 65 70 13405DNAArtificial
SequenceExpression vector 13gccgccacta tgaagttcct ggtgaatgtg
gccttggtgt tcatggtggt gtacatcagc 60ttcatctacg ctagccttaa gggtaccgag
ctcggatcca ctagtgaatt cgatatctct 120agagcggccg ctgactacaa
agacgatgac gacaagcttt caccaattca acacgatttt 180cctgcactag
tgatgatact cataattttg ggcgtgatgg cagggattat cggaactatc
240cttcttatct cttactgtat cagccgaatg acaaagaaaa gttcagttga
catccaatct 300cctgagggtg gtgacaacag tgtgcctttg agttctattg
agcagactcc taatgaagag 360tcctccaatg ttagcggcgg ccatcaccat
caccatcact gataa 40514411DNAArtificial SequenceExpression vector
14gccgccacta tgaagttcct ggtgaatgtg gccttggtgt tcatggtggt gtacatcagc
60ttcatctacg ctagccttaa gggtaccgag ctcggatcca ctagtgaatt cgatatctct
120agagcggccg ctgactacaa agacgatgac gacaagcttc aaagagttga
tcaccatttt 180aatgagccag tgactatagc cattattttg ggcatgatcg
ctggtatcgt tggaactatc 240cttctcattt attacttaat cagcctaata
acaaagaaaa tttcagctga caaacaacct 300cccaagagtg aaaacacgga
tgagccacca agtcctattg aacagattat tgttcaagaa 360gagcatgaca
gcattgttag cggcggccat caccatcacc atcactgata a 41115405DNAArtificial
SequenceExpression vector 15gccgccacta tgaagttcct ggtgaatgtg
gccttggtgt tcatggtggt gtacatcagc 60ttcatctacg ctagccttaa gggtaccgag
ctcggatcca ctagtgaatt cgatatcgac 120gtcgcggccg ctgactacaa
agacgatgac gacaagagcc ctagggccgc acacgatttt 180cctgcactag
tgatgatact cataattttg ggcgtgatgg cagggattat cggaactatc
240cttcttatct cttactgtat cagccgaatg acaaagaaaa gttcagttga
catccaatct 300cctgagggtg gtgacaacag tgtgcctttg agttctattg
agcagactcc taatgaagag 360tcctccaatg ttagcggcgg ccatcaccat
caccatcact gataa 405
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