U.S. patent application number 13/686320 was filed with the patent office on 2013-04-18 for nucleotide sequences encoding insecticidal proteins. This patent application is currently assigned to MONSANTO TECHNOLOGY LLC. The applicant listed for this patent is Monsanto Technology LLC. Invention is credited to Natalia N. Bogdanova, David R. Corbin, Thomas M. Malvar, Frederick J. Perlak, James K. Roberts, Charles P. Romano.
Application Number | 20130095488 13/686320 |
Document ID | / |
Family ID | 37728241 |
Filed Date | 2013-04-18 |
United States Patent Application | 20130095488 |
Kind Code | A1 |
Bogdanova; Natalia N. ; et al. | April 18, 2013 |
The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.
Inventors: | Bogdanova; Natalia N.; (Frontenac, MO) ; Corbin; David R.; (Chesterfield, MO) ; Malvar; Thomas M.; (North Stonington, CT) ; Perlak; Frederick J.; (St. Louis, MO) ; Roberts; James K.; (St. Louis, MO) ; Romano; Charles P.; (Chesterfield, MO) | ||||||||||
Applicant: |
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Assignee: | MONSANTO TECHNOLOGY LLC St. Louis MO |
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Family ID: | 37728241 | ||||||||||
Appl. No.: | 13/686320 | ||||||||||
Filed: | November 27, 2012 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
13114026 | May 23, 2011 | 8344207 | ||
13686320 | ||||
12064875 | Feb 26, 2008 | 8034997 | ||
PCT/US2006/033868 | Aug 30, 2006 | |||
13114026 | ||||
60713144 | Aug 31, 2005 | |||
Current U.S. Class: | 435/6.11 ; 436/501; 536/23.71 |
Current CPC Class: | C12N 15/8286 20130101; Y02A 40/146 20180101; C07K 14/325 20130101; Y02A 40/162 20180101 |
Class at Publication: | 435/6.11 ; 436/501; 536/23.71 |
International Class: | C07K 14/325 20060101 C07K014/325 |
Sequence CWU 1
1
813534DNAartificialsynthetic nucleotide sequence encoding Cry1A.105
amino acid sequence 1atg gac aac aac cca aac atc aac gaa tgc att
cca tac aac tgc ttg 48Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile
Pro Tyr Asn Cys Leu 1 5 10 15 agt aac cca gaa gtt gaa gta ctt ggt
gga gaa cgc att gaa acc ggt 96Ser Asn Pro Glu Val Glu Val Leu Gly
Gly Glu Arg Ile Glu Thr Gly 20 25 30 tac act ccc atc gac atc tcc
ttg tcc ttg aca cag ttt ctg ctc agc 144Tyr Thr Pro Ile Asp Ile Ser
Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45 gag ttc gtg cca ggt
gct ggg ttc gtt ctc gga cta gtt gac atc atc 192Glu Phe Val Pro Gly
Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50 55 60 tgg ggt atc
ttt ggt cca tct caa tgg gat gca ttc ctg gtg caa att 240Trp Gly Ile
Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70 75 80 gag
cag ttg atc aac cag agg atc gaa gag ttc gcc agg aac cag gcc 288Glu
Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90
95 atc tct agg ttg gaa gga ttg agc aat ctc tac caa atc tat gca gag
336Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110 agc ttc aga gag tgg gaa gcc gat cct act aac cca gct ctc
cgc gag 384Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu
Arg Glu 115 120 125 gaa atg cgt att caa ttc aac gac atg aac agc gcc
ttg acc aca gct 432Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala
Leu Thr Thr Ala 130 135 140 atc cca ttg ttc gca gtc cag aac tac caa
gtt cct ctc ttg tcc gtg 480Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln
Val Pro Leu Leu Ser Val 145 150 155 160 tac gtt caa gca gct aat ctt
cac ctc agc gtg ctt cga gac gtt agc 528Tyr Val Gln Ala Ala Asn Leu
His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 gtg ttt ggg caa agg
tgg gga ttc gat gct gca acc atc aat agc cgt 576Val Phe Gly Gln Arg
Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 tac aac gac
ctt act agg ctg att gga aac tac acc gac cac gct gtt 624Tyr Asn Asp
Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val 195 200 205 cgt
tgg tac aac act ggc ttg gag cgt gtc tgg ggt cct gat tct aga 672Arg
Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 210 215
220 gat tgg att aga tac aac cag ttc agg aga gaa ttg acc ctc aca gtt
720Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240 ttg gac att gtg tct ctc ttc ccg aac tat gac tcc aga
acc tac cct 768Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg
Thr Tyr Pro 245 250 255 atc cgt aca gtg tcc caa ctt acc aga gaa atc
tat act aac cca gtt 816Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile
Tyr Thr Asn Pro Val 260 265 270 ctt gag aac ttc gac ggt agc ttc cgt
ggt tct gcc caa ggt atc gaa 864Leu Glu Asn Phe Asp Gly Ser Phe Arg
Gly Ser Ala Gln Gly Ile Glu 275 280 285 ggc tcc atc agg agc cca cac
ttg atg gac atc ttg aac agc ata act 912Gly Ser Ile Arg Ser Pro His
Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 atc tac acc gat gct
cac aga gga gag tat tac tgg tct gga cac cag 960Ile Tyr Thr Asp Ala
His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 305 310 315 320 atc atg
gcc tct cca gtt gga ttc agc ggg ccc gag ttt acc ttt cct 1008Ile Met
Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 325 330 335
ctc tat gga act atg gga aac gcc gct cca caa caa cgt atc gtt gct
1056Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350 caa cta ggt cag ggt gtc tac aga acc ttg tct tcc acc ttg
tac aga 1104Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu
Tyr Arg 355 360 365 aga ccc ttc aat atc ggt atc aac aac cag caa ctt
tcc gtt ctt gac 1152Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu
Ser Val Leu Asp 370 375 380 gga aca gag ttc gcc tat gga acc tct tct
aac ttg cca tcc gct gtt 1200Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser
Asn Leu Pro Ser Ala Val 385 390 395 400 tac aga aag agc gga acc gtt
gat tcc ttg gac gaa atc cca cca cag 1248Tyr Arg Lys Ser Gly Thr Val
Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 aac aac aat gtg cca
ccc agg caa gga ttc tcc cac agg ttg agc cac 1296Asn Asn Asn Val Pro
Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430 gtg tcc atg
ttc cgt tcc gga ttc agc aac agt tcc gtg agc atc atc 1344Val Ser Met
Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440 445 aga
gct cct atg ttc tct tgg ata cac cgt agt gct gag ttc aac aac 1392Arg
Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 450 455
460 atc att gca tcc gac agc att act caa ata ccc ttg gtg aaa gca cat
1440Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His
465 470 475 480 aca ctt cag tca ggt act act gtt gtc aga ggt cca ggg
ttt aca gga 1488Thr Leu Gln Ser Gly Thr Thr Val Val Arg Gly Pro Gly
Phe Thr Gly 485 490 495 gga gac att ctt cgt cgc aca agt gga gga ccc
ttt gct tac act att 1536Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro
Phe Ala Tyr Thr Ile 500 505 510 gtt aac atc aat ggc caa ttg ccc caa
agg tat cgt gca aga atc cgc 1584Val Asn Ile Asn Gly Gln Leu Pro Gln
Arg Tyr Arg Ala Arg Ile Arg 515 520 525 tat gcc tct act aca aat ctc
agg atc tac gtg act gtt gca ggt gaa 1632Tyr Ala Ser Thr Thr Asn Leu
Arg Ile Tyr Val Thr Val Ala Gly Glu 530 535 540 agg atc ttt gct ggt
cag ttc aac aag act atg gat acc ggt gac cct 1680Arg Ile Phe Ala Gly
Gln Phe Asn Lys Thr Met Asp Thr Gly Asp Pro 545 550 555 560 ttg aca
ttc caa tct ttt agc tac gca act atc aac aca gct ttt aca 1728Leu Thr
Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr 565 570 575
ttc cca atg agc cag agt agc ttc aca gta ggt gct gac act ttc agc
1776Phe Pro Met Ser Gln Ser Ser Phe Thr Val Gly Ala Asp Thr Phe Ser
580 585 590 tca ggg aat gaa gtt tac atc gac agg ttt gaa ttg att cca
gtt act 1824Ser Gly Asn Glu Val Tyr Ile Asp Arg Phe Glu Leu Ile Pro
Val Thr 595 600 605 gca acc ctc gag gct gag tac aac ctt gag aga gcc
cag aag gct gtg 1872Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala
Gln Lys Ala Val 610 615 620 aac gcc ctc ttt acc tcc acc aat cag ctt
ggc ttg aaa act aac gtt 1920Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu
Gly Leu Lys Thr Asn Val 625 630 635 640 act gac tat cac att gac caa
gtg tcc aac ttg gtc acc tac ctt agc 1968Thr Asp Tyr His Ile Asp Gln
Val Ser Asn Leu Val Thr Tyr Leu Ser 645 650 655 gat gag ttc tgc ctc
gac gag aag cgt gaa ctc tcc gag aaa gtt aaa 2016Asp Glu Phe Cys Leu
Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 660 665 670 cac gcc aag
cgt ctc agc gac gag agg aat ctc ttg caa gac tcc aac 2064His Ala Lys
Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn 675 680 685 ttc
aaa gac atc aac agg cag cca gaa cgt ggt tgg ggt gga agc acc 2112Phe
Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser Thr 690 695
700 ggg atc acc atc caa gga ggc gac gat gtg ttc aag gag aac tac gtc
2160Gly Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720 acc ctc tcc gga act ttc gac gag tgc tac cct acc tac
ttg tac cag 2208Thr Leu Ser Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr
Leu Tyr Gln 725 730 735 aag atc gat gag tcc aaa ctc aaa gcc ttc acc
agg tat caa ctt aga 2256Lys Ile Asp Glu Ser Lys Leu Lys Ala Phe Thr
Arg Tyr Gln Leu Arg 740 745 750 ggc tac atc gaa gac agc caa gac ctt
gaa atc tac tcg atc agg tac 2304Gly Tyr Ile Glu Asp Ser Gln Asp Leu
Glu Ile Tyr Ser Ile Arg Tyr 755 760 765 aat gcc aag cac gag acc gtg
aat gtc cca ggt act ggt tcc ctc tgg 2352Asn Ala Lys His Glu Thr Val
Asn Val Pro Gly Thr Gly Ser Leu Trp 770 775 780 cca ctt tct gcc caa
tct ccc att ggg aag tgt gga gag cct aac aga 2400Pro Leu Ser Ala Gln
Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn Arg 785 790 795 800 tgc gct
cca cac ctt gag tgg aat cct gac ttg gac tgc tcc tgc agg 2448Cys Ala
Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg 805 810 815
gat ggc gag aag tgt gcc cac cat tct cat cac ttc tcc ttg gac atc
2496Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile
820 825 830 gat gtg gga tgt act gac ctg aat gag gac ctc gga gtc tgg
gtc atc 2544Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp
Val Ile 835 840 845 ttc aag atc aag acc caa gac gga cac gca aga ctt
ggc aac ctt gag 2592Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu
Gly Asn Leu Glu 850 855 860 ttt ctc gaa gag aaa cca ttg gtc ggt gaa
gct ctc gct cgt gtg aag 2640Phe Leu Glu Glu Lys Pro Leu Val Gly Glu
Ala Leu Ala Arg Val Lys 865 870 875 880 aga gca gag aag aag tgg agg
gac aaa cgt gag aaa ctc gaa tgg gaa 2688Arg Ala Glu Lys Lys Trp Arg
Asp Lys Arg Glu Lys Leu Glu Trp Glu 885 890 895 act aac atc gtt tac
aag gag gcc aaa gag tcc gtg gat gct ttg ttc 2736Thr Asn Ile Val Tyr
Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 900 905 910 gtg aac tcc
caa tat gat cag ttg caa gcc gac acc aac atc gcc atg 2784Val Asn Ser
Gln Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala Met 915 920 925 atc
cac gcc gca gac aaa cgt gtg cac agc att cgt gag gct tac ttg 2832Ile
His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu 930 935
940 cct gag ttg tcc gtg atc cct ggt gtg aac gct gcc atc ttc gag gaa
2880Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu
945 950 955 960 ctt gag gga cgt atc ttt acc gca ttc tcc ttg tac gat
gcc aga aac 2928Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp
Ala Arg Asn 965 970 975 gtc atc aag aac ggt gac ttc aac aat ggc ctc
agc tgc tgg aat gtg 2976Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu
Ser Cys Trp Asn Val 980 985 990 aaa ggt cat gtg gac gtg gag gaa cag
aac aat cag cgt tcc gtc ctg 3024Lys Gly His Val Asp Val Glu Glu Gln
Asn Asn Gln Arg Ser Val Leu 995 1000 1005 gtt gtg cct gag tgg gaa
gct gaa gtg tcc caa gag gtt aga gtc 3069Val Val Pro Glu Trp Glu Ala
Glu Val Ser Gln Glu Val Arg Val 1010 1015 1020 tgt cca ggt aga ggc
tac att ctc cgt gtg acc gct tac aag gag 3114Cys Pro Gly Arg Gly Tyr
Ile Leu Arg Val Thr Ala Tyr Lys Glu 1025 1030 1035 gga tac ggt gag
ggt tgc gtg acc atc cac gag atc gag aac aac 3159Gly Tyr Gly Glu Gly
Cys Val Thr Ile His Glu Ile Glu Asn Asn 1040 1045 1050 acc gac gag
ctt aag ttc tcc aac tgc gtc gag gaa gaa atc tat 3204Thr Asp Glu Leu
Lys Phe Ser Asn Cys Val Glu Glu Glu Ile Tyr 1055 1060 1065 ccc aac
aac acc gtt act tgc aac gac tac act gtg aat cag gaa 3249Pro Asn Asn
Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln Glu 1070 1075 1080 gag
tac gga ggt gcc tac act agc cgt aac aga ggt tac aac gaa 3294Glu Tyr
Gly Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn Glu 1085 1090 1095
gct cct tcc gtt cct gct gac tat gcc tcc gtg tac gag gag aaa 3339Ala
Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu Lys 1100 1105
1110 tcc tac aca gat ggc aga cgt gag aac cct tgc gag ttc aac aga
3384Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu Phe Asn Arg
1115 1120 1125 ggt tac agg gac tac aca cca ctt cca gtt ggc tat gtt
acc aag 3429Gly Tyr Arg Asp Tyr Thr Pro Leu Pro Val Gly Tyr Val Thr
Lys 1130 1135 1140 gag ctt gag tac ttt cct gag acc gac aaa gtg tgg
atc gag atc 3474Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile
Glu Ile 1145 1150 1155 ggt gaa acc gag gga acc ttc atc gtg gac agc
gtg gag ctt ctc 3519Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val
Glu Leu Leu 1160 1165 1170 ttg atg gag gaa taa 3534Leu Met Glu Glu
1175 21177PRTartificialSynthetic Construct 2Met Asp Asn Asn Pro Asn
Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 Ser Asn Pro Glu
Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 Tyr Thr
Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50
55 60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln
Ile 65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg
Asn Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr
Gln Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu Trp Glu Ala Asp Pro
Thr Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met Arg Ile Gln Phe Asn
Asp Met Asn Ser Ala Leu Thr Thr Ala 130 135 140 Ile Pro Leu Phe Ala
Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 Tyr Val
Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180
185 190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala
Val 195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro
Asp Ser Arg 210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 225
230 235 240 Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr
Tyr Pro 245 250 255 Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr
Thr Asn Pro Val 260 265 270 Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly
Ser Ala Gln Gly Ile Glu 275 280 285 Gly Ser Ile Arg Ser Pro His Leu
Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 Ile Tyr Thr Asp Ala His
Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 305 310 315 320 Ile Met Ala
Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 325 330 335 Leu
Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala 340 345
350 Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365 Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val
Leu Asp 370 375 380 Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu
Pro Ser Ala Val 385 390 395 400 Tyr Arg Lys Ser Gly Thr Val Asp Ser
Leu Asp Glu Ile Pro Pro Gln 405 410 415 Asn Asn Asn Val Pro Pro Arg
Gln Gly Phe Ser His Arg Leu Ser His 420 425 430 Val Ser Met Phe Arg
Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440 445 Arg Ala Pro
Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 450 455 460 Ile
Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His 465 470
475 480 Thr Leu Gln Ser Gly Thr Thr Val Val Arg Gly Pro Gly Phe Thr
Gly 485 490 495 Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala
Tyr Thr Ile 500 505 510 Val Asn Ile Asn Gly Gln Leu Pro Gln Arg Tyr
Arg Ala Arg Ile Arg 515 520 525 Tyr Ala Ser Thr Thr Asn Leu Arg Ile
Tyr Val Thr Val Ala Gly Glu 530 535 540 Arg Ile Phe Ala Gly Gln Phe
Asn Lys Thr Met Asp Thr Gly Asp Pro 545 550 555 560 Leu Thr Phe Gln
Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr 565 570 575 Phe Pro
Met Ser Gln Ser Ser Phe Thr Val Gly Ala Asp Thr Phe Ser 580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Phe Glu Leu Ile Pro Val Thr 595
600 605 Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala Gln Lys Ala
Val 610 615 620 Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys
Thr Asn Val 625 630 635 640 Thr Asp Tyr His Ile Asp Gln Val Ser Asn
Leu Val Thr Tyr Leu Ser 645 650 655 Asp Glu Phe Cys Leu Asp Glu Lys
Arg Glu Leu Ser Glu Lys Val Lys 660 665 670 His Ala Lys Arg Leu Ser
Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn 675 680 685 Phe Lys Asp Ile
Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser Thr 690 695 700 Gly Ile
Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 705 710 715
720 Thr Leu Ser Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735 Lys Ile Asp Glu Ser Lys Leu Lys Ala Phe Thr Arg Tyr Gln
Leu Arg 740 745 750 Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr
Ser Ile Arg Tyr 755 760 765 Asn Ala Lys His Glu Thr Val Asn Val Pro
Gly Thr Gly Ser Leu Trp 770 775 780 Pro Leu Ser Ala Gln Ser Pro Ile
Gly Lys Cys Gly Glu Pro Asn Arg 785 790 795 800 Cys Ala Pro His Leu
Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg 805 810 815 Asp Gly Glu
Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 820 825 830 Asp
Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 835 840
845 Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu
850 855 860 Phe Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg
Val Lys 865 870 875 880 Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu
Lys Leu Glu Trp Glu 885 890 895 Thr Asn Ile Val Tyr Lys Glu Ala Lys
Glu Ser Val Asp Ala Leu Phe 900 905 910 Val Asn Ser Gln Tyr Asp Gln
Leu Gln Ala Asp Thr Asn Ile Ala Met 915 920 925 Ile His Ala Ala Asp
Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu 930 935 940 Pro Glu Leu
Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 945 950 955 960
Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 965
970 975 Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn
Val 980 985 990 Lys Gly His Val Asp Val Glu Glu Gln Asn Asn Gln Arg
Ser Val Leu 995 1000 1005 Val Val Pro Glu Trp Glu Ala Glu Val Ser
Gln Glu Val Arg Val 1010 1015 1020 Cys Pro Gly Arg Gly Tyr Ile Leu
Arg Val Thr Ala Tyr Lys Glu 1025 1030 1035 Gly Tyr Gly Glu Gly Cys
Val Thr Ile His Glu Ile Glu Asn Asn 1040 1045 1050 Thr Asp Glu Leu
Lys Phe Ser Asn Cys Val Glu Glu Glu Ile Tyr 1055 1060 1065 Pro Asn
Asn Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln Glu 1070 1075 1080
Glu Tyr Gly Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn Glu 1085
1090 1095 Ala Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu
Lys 1100 1105 1110 Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu
Phe Asn Arg 1115 1120 1125 Gly Tyr Arg Asp Tyr Thr Pro Leu Pro Val
Gly Tyr Val Thr Lys 1130 1135 1140 Glu Leu Glu Tyr Phe Pro Glu Thr
Asp Lys Val Trp Ile Glu Ile 1145 1150 1155 Gly Glu Thr Glu Gly Thr
Phe Ile Val Asp Ser Val Glu Leu Leu 1160 1165 1170 Leu Met Glu Glu
1175 33534DNAartificialsynthetic nucleotide sequence encoding
Cry1A.105 amino acid sequence 3atg gac aac aac cca aac atc aac gag
tgc atc ccg tac aac tgc ctc 48Met Asp Asn Asn Pro Asn Ile Asn Glu
Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 agc aac cct gag gtc gag gtg
ctc ggc ggt gag cgc atc gag acc ggt 96Ser Asn Pro Glu Val Glu Val
Leu Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 tac acc ccc atc gac
atc tcc ctc tcc ctc acg cag ttc ctg ctc agc 144Tyr Thr Pro Ile Asp
Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45 gag ttc gtg
cca ggc gct ggc ttc gtc ctg ggc ctc gtg gac atc atc 192Glu Phe Val
Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50 55 60 tgg
ggc atc ttt ggc ccc tcc cag tgg gac gcc ttc ctg gtg caa atc 240Trp
Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70
75 80 gag cag ctc atc aac cag agg atc gag gag ttc gcc agg aac cag
gcc 288Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln
Ala 85 90 95 atc agc cgc ctg gag ggc ctc agc aac ctc tac caa atc
tac gct gag 336Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile
Tyr Ala Glu 100 105 110 agc ttc cgc gag tgg gag gcc gac ccc act aac
cca gct ctc cgc gag 384Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn
Pro Ala Leu Arg Glu 115 120 125 gag atg cgc atc cag ttc aac gac atg
aac agc gcc ctg acc acc gcc 432Glu Met Arg Ile Gln Phe Asn Asp Met
Asn Ser Ala Leu Thr Thr Ala 130 135 140 atc cca ctc ttc gcc gtc cag
aac tac caa gtc ccg ctc ctg tcc gtg 480Ile Pro Leu Phe Ala Val Gln
Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 tac gtc cag gcc
gcc aac ctg cac ctc agc gtg ctg agg gac gtc agc 528Tyr Val Gln Ala
Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 gtg ttt
ggc cag agg tgg ggc ttc gac gcc gcc acc atc aac agc cgc 576Val Phe
Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190
tac aac gac ctc acc agg ctg atc ggc aac tac acc gac cac gct gtc
624Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205 cgc tgg tac aac act ggc ctg gag cgc gtc tgg ggc cct gat
tct aga 672Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp
Ser Arg 210 215 220 gac tgg att cgc tac aac cag ttc agg cgc gag ctg
acc ctc acc gtc 720Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu
Thr Leu Thr Val 225 230 235 240 ctg gac att gtg tcc ctc ttc ccg aac
tac gac tcc cgc acc tac ccg 768Leu Asp Ile Val Ser Leu Phe Pro Asn
Tyr Asp Ser Arg Thr Tyr Pro 245 250 255 atc cgc acc gtg tcc caa ctg
acc cgc gaa atc tac acc aac ccc gtc 816Ile Arg Thr Val Ser Gln Leu
Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265 270 ctg gag aac ttc gac
ggt agc ttc agg ggc agc gcc cag ggc atc gag 864Leu Glu Asn Phe Asp
Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 ggc tcc atc
agg agc cca cac ctg atg gac atc ctc aac agc atc act 912Gly Ser Ile
Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 atc
tac acc gat gcc cac cgc ggc gag tac tac tgg tcc ggc cac cag 960Ile
Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 305 310
315 320 atc atg gcc tcc ccg gtc ggc ttc agc ggc ccc gag ttt acc ttt
cct 1008Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe
Pro 325 330 335 ctc tac ggc acg atg ggc aac gcc gct cca caa caa cgc
atc gtc gct 1056Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg
Ile Val Ala 340 345 350 cag ctg ggc cag ggc gtc tac cgc acc ctg agc
tcc acc ctg tac cgc 1104Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser
Ser Thr Leu Tyr Arg 355 360 365 agg ccc ttc aac atc ggt atc aac aac
cag cag ctg tcc gtc ctg gat 1152Arg Pro Phe Asn Ile Gly Ile Asn Asn
Gln Gln Leu Ser Val Leu Asp 370 375 380 ggc act gag ttc gcc tac ggc
acc tcc tcc aac ctg ccc tcc gct gtc 1200Gly Thr Glu Phe Ala Tyr Gly
Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400 tac cgc aag agc
ggc acg gtg gat tcc ctg gac gag atc cca cca cag 1248Tyr Arg Lys Ser
Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 aac aac
aat gtg ccc ccc agg cag ggt ttt tcc cac agg ctc agc cac 1296Asn Asn
Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430
gtg tcc atg ttc cgc tcc ggc ttc agc aac tcg tcc gtg agc atc atc
1344Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445 aga gct cct atg ttc tct tgg ata cac cgt agt gct gag ttc
aac aac 1392Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe
Asn Asn 450 455 460 atc att gca tcc gac agc att act caa ata ccc ttg
gtg aaa gca cat 1440Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Leu
Val Lys Ala His 465 470 475 480 aca ctt cag tca ggt act act gtt gtc
aga ggt cca ggg ttt aca gga 1488Thr Leu Gln Ser Gly Thr Thr Val Val
Arg Gly Pro Gly Phe Thr Gly 485 490 495 gga gac att ctt cgt cgc aca
agt gga gga ccc ttt gct tac act att 1536Gly Asp Ile Leu Arg Arg Thr
Ser Gly Gly Pro Phe Ala Tyr Thr Ile 500 505 510 gtt aac atc aat ggc
caa ttg ccc caa agg tat cgt gca aga atc cgc 1584Val Asn Ile Asn Gly
Gln Leu Pro Gln Arg Tyr Arg Ala Arg Ile Arg 515 520 525 tat gcc tct
act aca aat ctc agg atc tac gtg act gtt gca ggt gaa 1632Tyr Ala Ser
Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu 530 535 540 agg
atc ttt gct ggt cag ttc aac aag act atg gat acc ggt gac cct 1680Arg
Ile Phe Ala Gly Gln Phe Asn Lys Thr Met Asp Thr Gly Asp Pro 545 550
555 560 ttg aca ttc caa tct ttt agc tac gca act atc aac aca gct ttt
aca 1728Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe
Thr 565 570 575 ttc cca atg agc cag agt agc ttc aca gta ggt gct gac
act ttc agc 1776Phe Pro Met Ser Gln Ser Ser Phe Thr Val Gly Ala Asp
Thr Phe Ser 580 585 590 tca ggg aat gaa gtt tac atc gac agg ttt gaa
ttg att cca gtt act 1824Ser Gly Asn Glu Val Tyr Ile Asp Arg Phe Glu
Leu Ile Pro Val Thr 595 600 605 gca acc ctc gag gct gag tac aac ctt
gag aga gcc cag aag gct gtg 1872Ala Thr Leu Glu Ala Glu Tyr Asn Leu
Glu Arg Ala Gln Lys Ala Val 610 615 620 aac gcc ctc ttt acc tcc acc
aat cag ctt ggc ttg aaa act aac gtt 1920Asn Ala Leu Phe Thr Ser Thr
Asn Gln Leu Gly Leu Lys Thr Asn Val 625 630 635 640 act gac tat cac
att gac caa gtg tcc aac ttg gtc acc tac ctt agc 1968Thr Asp Tyr His
Ile Asp Gln Val Ser Asn Leu Val Thr Tyr Leu Ser 645 650 655 gat gag
ttc tgc ctc gac gag aag cgt gaa ctc tcc gag aaa gtt aaa 2016Asp Glu
Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 660 665 670
cac gcc aag cgt ctc agc gac gag agg aat ctc ttg caa gac tcc aac
2064His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn
675 680 685 ttc aaa gac atc aac agg cag cca gaa cgt ggt tgg ggt gga
agc acc 2112Phe Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly
Ser Thr 690 695 700 ggg atc acc atc caa gga ggc gac gat gtg ttc aag
gag aac tac gtc 2160Gly Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys
Glu Asn Tyr Val 705 710 715 720 acc ctc tcc gga act ttc gac gag tgc
tac cct acc tac ttg tac cag 2208Thr Leu Ser Gly Thr Phe Asp Glu Cys
Tyr Pro Thr Tyr Leu Tyr Gln 725 730 735 aag atc gat gag tcc aaa ctc
aaa gcc ttc acc agg tat caa ctt aga 2256Lys Ile Asp Glu Ser Lys Leu
Lys Ala Phe Thr Arg Tyr Gln Leu Arg 740 745 750 ggc tac atc gaa gac
agc caa gac ctt gaa atc tac tcg atc agg tac 2304Gly Tyr Ile Glu Asp
Ser Gln Asp Leu Glu Ile Tyr Ser Ile Arg Tyr 755 760 765 aat gcc aag
cac gag acc gtg aat gtc cca ggt act ggt tcc ctc tgg
2352Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780 cca ctt tct gcc caa tct ccc att ggg aag tgt gga gag cct
aac aga 2400Pro Leu Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro
Asn Arg 785 790 795 800 tgc gct cca cac ctt gag tgg aat cct gac ttg
gac tgc tcc tgc agg 2448Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu
Asp Cys Ser Cys Arg 805 810 815 gat ggc gag aag tgt gcc cac cat tct
cat cac ttc tcc ttg gac atc 2496Asp Gly Glu Lys Cys Ala His His Ser
His His Phe Ser Leu Asp Ile 820 825 830 gat gtg gga tgt act gac ctg
aat gag gac ctc gga gtc tgg gtc atc 2544Asp Val Gly Cys Thr Asp Leu
Asn Glu Asp Leu Gly Val Trp Val Ile 835 840 845 ttc aag atc aag acc
caa gac gga cac gca aga ctt ggc aac ctt gag 2592Phe Lys Ile Lys Thr
Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 850 855 860 ttt ctc gaa
gag aaa cca ttg gtc ggt gaa gct ctc gct cgt gtg aag 2640Phe Leu Glu
Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys 865 870 875 880
aga gca gag aag aag tgg agg gac aaa cgt gag aaa ctc gaa tgg gaa
2688Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu
885 890 895 act aac atc gtt tac aag gag gcc aaa gag tcc gtg gat gct
ttg ttc 2736Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala
Leu Phe 900 905 910 gtg aac tcc caa tat gat cag ttg caa gcc gac acc
aac atc gcc atg 2784Val Asn Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr
Asn Ile Ala Met 915 920 925 atc cac gcc gca gac aaa cgt gtg cac agc
att cgt gag gct tac ttg 2832Ile His Ala Ala Asp Lys Arg Val His Ser
Ile Arg Glu Ala Tyr Leu 930 935 940 cct gag ttg tcc gtg atc cct ggt
gtg aac gct gcc atc ttc gag gaa 2880Pro Glu Leu Ser Val Ile Pro Gly
Val Asn Ala Ala Ile Phe Glu Glu 945 950 955 960 ctt gag gga cgt atc
ttt acc gca ttc tcc ttg tac gat gcc aga aac 2928Leu Glu Gly Arg Ile
Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 965 970 975 gtc atc aag
aac ggt gac ttc aac aat ggc ctc agc tgc tgg aat gtg 2976Val Ile Lys
Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val 980 985 990 aaa
ggt cat gtg gac gtg gag gaa cag aac aat cag cgt tcc gtc ctg 3024Lys
Gly His Val Asp Val Glu Glu Gln Asn Asn Gln Arg Ser Val Leu 995
1000 1005 gtt gtg cct gag tgg gaa gct gaa gtg tcc caa gag gtt aga
gtc 3069Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val
1010 1015 1020 tgt cca ggt aga ggc tac att ctc cgt gtg acc gct tac
aag gag 3114Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys
Glu 1025 1030 1035 gga tac ggt gag ggt tgc gtg acc atc cac gag atc
gag aac aac 3159Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu
Asn Asn 1040 1045 1050 acc gac gag ctt aag ttc tcc aac tgc gtc gag
gaa gaa atc tat 3204Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu
Glu Ile Tyr 1055 1060 1065 ccc aac aac acc gtt act tgc aac gac tac
act gtg aat cag gaa 3249Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr
Val Asn Gln Glu 1070 1075 1080 gag tac gga ggt gcc tac act agc cgt
aac aga ggt tac aac gaa 3294Glu Tyr Gly Gly Ala Tyr Thr Ser Arg Asn
Arg Gly Tyr Asn Glu 1085 1090 1095 gct cct tcc gtt cct gct gac tat
gcc tcc gtg tac gag gag aaa 3339Ala Pro Ser Val Pro Ala Asp Tyr Ala
Ser Val Tyr Glu Glu Lys 1100 1105 1110 tcc tac aca gat ggc aga cgt
gag aac cct tgc gag ttc aac aga 3384Ser Tyr Thr Asp Gly Arg Arg Glu
Asn Pro Cys Glu Phe Asn Arg 1115 1120 1125 ggt tac agg gac tac aca
cca ctt cca gtt ggc tat gtt acc aag 3429Gly Tyr Arg Asp Tyr Thr Pro
Leu Pro Val Gly Tyr Val Thr Lys 1130 1135 1140 gag ctt gag tac ttt
cct gag acc gac aaa gtg tgg atc gag atc 3474Glu Leu Glu Tyr Phe Pro
Glu Thr Asp Lys Val Trp Ile Glu Ile 1145 1150 1155 ggt gaa acc gag
gga acc ttc atc gtg gac agc gtg gag ctt ctc 3519Gly Glu Thr Glu Gly
Thr Phe Ile Val Asp Ser Val Glu Leu Leu 1160 1165 1170 ttg atg gag
gaa taa 3534Leu Met Glu Glu 1175 41177PRTartificialSynthetic
Construct 4Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn
Cys Leu 1 5 10 15 Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg
Ile Glu Thr Gly 20 25 30 Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu
Thr Gln Phe Leu Leu Ser 35 40 45 Glu Phe Val Pro Gly Ala Gly Phe
Val Leu Gly Leu Val Asp Ile Ile 50 55 60 Trp Gly Ile Phe Gly Pro
Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70 75 80 Glu Gln Leu Ile
Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95 Ile Ser
Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu 115
120 125 Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr
Ala 130 135 140 Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu
Leu Ser Val 145 150 155 160 Tyr Val Gln Ala Ala Asn Leu His Leu Ser
Val Leu Arg Asp Val Ser 165 170 175 Val Phe Gly Gln Arg Trp Gly Phe
Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 Tyr Asn Asp Leu Thr Arg
Leu Ile Gly Asn Tyr Thr Asp His Ala Val 195 200 205 Arg Trp Tyr Asn
Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 210 215 220 Asp Trp
Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 225 230 235
240 Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255 Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn
Pro Val 260 265 270 Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala
Gln Gly Ile Glu 275 280 285 Gly Ser Ile Arg Ser Pro His Leu Met Asp
Ile Leu Asn Ser Ile Thr 290 295 300 Ile Tyr Thr Asp Ala His Arg Gly
Glu Tyr Tyr Trp Ser Gly His Gln 305 310 315 320 Ile Met Ala Ser Pro
Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 325 330 335 Leu Tyr Gly
Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala 340 345 350 Gln
Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 355 360
365 Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380 Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser
Ala Val 385 390 395 400 Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp
Glu Ile Pro Pro Gln 405 410 415 Asn Asn Asn Val Pro Pro Arg Gln Gly
Phe Ser His Arg Leu Ser His 420 425 430 Val Ser Met Phe Arg Ser Gly
Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440 445 Arg Ala Pro Met Phe
Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 450 455 460 Ile Ile Ala
Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His 465 470 475 480
Thr Leu Gln Ser Gly Thr Thr Val Val Arg Gly Pro Gly Phe Thr Gly 485
490 495 Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Tyr Thr
Ile 500 505 510 Val Asn Ile Asn Gly Gln Leu Pro Gln Arg Tyr Arg Ala
Arg Ile Arg 515 520 525 Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val
Thr Val Ala Gly Glu 530 535 540 Arg Ile Phe Ala Gly Gln Phe Asn Lys
Thr Met Asp Thr Gly Asp Pro 545 550 555 560 Leu Thr Phe Gln Ser Phe
Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr 565 570 575 Phe Pro Met Ser
Gln Ser Ser Phe Thr Val Gly Ala Asp Thr Phe Ser 580 585 590 Ser Gly
Asn Glu Val Tyr Ile Asp Arg Phe Glu Leu Ile Pro Val Thr 595 600 605
Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala Gln Lys Ala Val 610
615 620 Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asn
Val 625 630 635 640 Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val
Thr Tyr Leu Ser 645 650 655 Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu
Leu Ser Glu Lys Val Lys 660 665 670 His Ala Lys Arg Leu Ser Asp Glu
Arg Asn Leu Leu Gln Asp Ser Asn 675 680 685 Phe Lys Asp Ile Asn Arg
Gln Pro Glu Arg Gly Trp Gly Gly Ser Thr 690 695 700 Gly Ile Thr Ile
Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 705 710 715 720 Thr
Leu Ser Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 725 730
735 Lys Ile Asp Glu Ser Lys Leu Lys Ala Phe Thr Arg Tyr Gln Leu Arg
740 745 750 Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Ser Ile
Arg Tyr 755 760 765 Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr
Gly Ser Leu Trp 770 775 780 Pro Leu Ser Ala Gln Ser Pro Ile Gly Lys
Cys Gly Glu Pro Asn Arg 785 790 795 800 Cys Ala Pro His Leu Glu Trp
Asn Pro Asp Leu Asp Cys Ser Cys Arg 805 810 815 Asp Gly Glu Lys Cys
Ala His His Ser His His Phe Ser Leu Asp Ile 820 825 830 Asp Val Gly
Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 835 840 845 Phe
Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 850 855
860 Phe Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys
865 870 875 880 Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu
Glu Trp Glu 885 890 895 Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser
Val Asp Ala Leu Phe 900 905 910 Val Asn Ser Gln Tyr Asp Gln Leu Gln
Ala Asp Thr Asn Ile Ala Met 915 920 925 Ile His Ala Ala Asp Lys Arg
Val His Ser Ile Arg Glu Ala Tyr Leu 930 935 940 Pro Glu Leu Ser Val
Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 945 950 955 960 Leu Glu
Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 965 970 975
Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val 980
985 990 Lys Gly His Val Asp Val Glu Glu Gln Asn Asn Gln Arg Ser Val
Leu 995 1000 1005 Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu
Val Arg Val 1010 1015 1020 Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val
Thr Ala Tyr Lys Glu 1025 1030 1035 Gly Tyr Gly Glu Gly Cys Val Thr
Ile His Glu Ile Glu Asn Asn 1040 1045 1050 Thr Asp Glu Leu Lys Phe
Ser Asn Cys Val Glu Glu Glu Ile Tyr 1055 1060 1065 Pro Asn Asn Thr
Val Thr Cys Asn Asp Tyr Thr Val Asn Gln Glu 1070 1075 1080 Glu Tyr
Gly Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn Glu 1085 1090 1095
Ala Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu Lys 1100
1105 1110 Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu Phe Asn
Arg 1115 1120 1125 Gly Tyr Arg Asp Tyr Thr Pro Leu Pro Val Gly Tyr
Val Thr Lys 1130 1135 1140 Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys
Val Trp Ile Glu Ile 1145 1150 1155 Gly Glu Thr Glu Gly Thr Phe Ile
Val Asp Ser Val Glu Leu Leu 1160 1165 1170 Leu Met Glu Glu 1175
55480DNAartificialexpression cassette encoding Cry1A.105 amino acid
sequence 5aattctcagt ccaaagcctc aacaaggtca gggtacagag tctccaaacc
attagccaaa 60agctacagga gatcaatgaa gaatcttcaa tcaaagtaaa ctactgttcc
agcacatgca 120tcatggtcag taagtttcag aaaaagacat ccaccgaaga
cttaaagtta gtgggcatct 180ttgaaagtaa tcttgtcaac atcgagcagc
tggcttgtgg ggaccagaca aaaaaggaat 240ggtgcagaat tgttaggcgc
acctaccaaa agcatctttg cctttattgc aaagataaag 300cagattcctc
tagtacaagt ggggaacaaa ataacgtgga aaagagctgt cctgacagcc
360cactcactaa tgcgtatgac gaacgcagtg acgaccacaa aagaattagc
ttgagctcag 420gatttagcag cattccagat tgggttcaat caacaaggta
cgagccatat cactttattc 480aaattggtat cgccaaaacc aagaaggaac
tcccatcctc aaaggtttgt aaggaagaat 540tctcagtcca aagcctcaac
aaggtcaggg tacagagtct ccaaaccatt agccaaaagc 600tacaggagat
caatgaagaa tcttcaatca aagtaaacta ctgttccagc acatgcatca
660tggtcagtaa gtttcagaaa aagacatcca ccgaagactt aaagttagtg
ggcatctttg 720aaagtaatct tgtcaacatc gagcagctgg cttgtgggga
ccagacaaaa aaggaatggt 780gcagaattgt taggcgcacc taccaaaagc
atctttgcct ttattgcaaa gataaagcag 840attcctctag tacaagtggg
gaacaaaata acgtggaaaa gagctgtcct gacagcccac 900tcactaatgc
gtatgacgaa cgcagtgacg accacaaaag aattccctct atataagaag
960gcattcattc ccatttgaag gacacagaaa aatttgctac attgtttcac
aaacttcaaa 1020tattattcat ttatttgtca gctttcaaac tctttgtttc
ttgtttgttg attgagaata 1080tttaaaacaa tggcttcctc tatgctctct
tccgctacta tggttgcctc tccggctcag 1140gccactatgg tcgctccttt
caacggactt aagtcctccg ctgccttccc agccacccgc 1200aaggctaaca
acgacattac ttccatcaca agcaacggcg gaagagttaa ctgcatgcag 1260gccatg
gac aac aac cca aac atc aac gaa tgc att cca tac aac tgc 1308 Asp
Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys 1 5 10 ttg agt
aac cca gaa gtt gaa gta ctt ggt gga gaa cgc att gaa acc 1356Leu Ser
Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr 15 20 25 30
ggt tac act ccc atc gac atc tcc ttg tcc ttg aca cag ttt ctg ctc
1404Gly Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu
35 40 45 agc gag ttc gtg cca ggt gct ggg ttc gtt ctc gga cta gtt
gac atc 1452Ser Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val
Asp Ile 50 55 60 atc tgg ggt atc ttt ggt cca tct caa tgg gat gca
ttc ctg gtg caa 1500Ile Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala
Phe Leu Val Gln 65 70 75 att gag cag ttg atc aac cag agg atc gaa
gag ttc gcc agg aac cag 1548Ile Glu Gln Leu Ile Asn Gln Arg Ile Glu
Glu Phe Ala Arg Asn Gln 80 85 90 gcc atc tct agg ttg gaa gga ttg
agc aat ctc tac caa atc tat gca
1596Ala Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala
95 100 105 110 gag agc ttc aga gag tgg gaa gcc gat cct act aac cca
gct ctc cgc 1644Glu Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro
Ala Leu Arg 115 120 125 gag gaa atg cgt att caa ttc aac gac atg aac
agc gcc ttg acc aca 1692Glu Glu Met Arg Ile Gln Phe Asn Asp Met Asn
Ser Ala Leu Thr Thr 130 135 140 gct atc cca ttg ttc gca gtc cag aac
tac caa gtt cct ctc ttg tcc 1740Ala Ile Pro Leu Phe Ala Val Gln Asn
Tyr Gln Val Pro Leu Leu Ser 145 150 155 gtg tac gtt caa gca gct aat
ctt cac ctc agc gtg ctt cga gac gtt 1788Val Tyr Val Gln Ala Ala Asn
Leu His Leu Ser Val Leu Arg Asp Val 160 165 170 agc gtg ttt ggg caa
agg tgg gga ttc gat gct gca acc atc aat agc 1836Ser Val Phe Gly Gln
Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser 175 180 185 190 cgt tac
aac gac ctt act agg ctg att gga aac tac acc gac cac gct 1884Arg Tyr
Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala 195 200 205
gtt cgt tgg tac aac act ggc ttg gag cgt gtc tgg ggt cct gat tct
1932Val Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser
210 215 220 aga gat tgg att aga tac aac cag ttc agg aga gaa ttg acc
ctc aca 1980Arg Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr
Leu Thr 225 230 235 gtt ttg gac att gtg tct ctc ttc ccg aac tat gac
tcc aga acc tac 2028Val Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp
Ser Arg Thr Tyr 240 245 250 cct atc cgt aca gtg tcc caa ctt acc aga
gaa atc tat act aac cca 2076Pro Ile Arg Thr Val Ser Gln Leu Thr Arg
Glu Ile Tyr Thr Asn Pro 255 260 265 270 gtt ctt gag aac ttc gac ggt
agc ttc cgt ggt tct gcc caa ggt atc 2124Val Leu Glu Asn Phe Asp Gly
Ser Phe Arg Gly Ser Ala Gln Gly Ile 275 280 285 gaa ggc tcc atc agg
agc cca cac ttg atg gac atc ttg aac agc ata 2172Glu Gly Ser Ile Arg
Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile 290 295 300 act atc tac
acc gat gct cac aga gga gag tat tac tgg tct gga cac 2220Thr Ile Tyr
Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His 305 310 315 cag
atc atg gcc tct cca gtt gga ttc agc ggg ccc gag ttt acc ttt 2268Gln
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe 320 325
330 cct ctc tat gga act atg gga aac gcc gct cca caa caa cgt atc gtt
2316Pro Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val
335 340 345 350 gct caa cta ggt cag ggt gtc tac aga acc ttg tct tcc
acc ttg tac 2364Ala Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser
Thr Leu Tyr 355 360 365 aga aga ccc ttc aat atc ggt atc aac aac cag
caa ctt tcc gtt ctt 2412Arg Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln
Gln Leu Ser Val Leu 370 375 380 gac gga aca gag ttc gcc tat gga acc
tct tct aac ttg cca tcc gct 2460Asp Gly Thr Glu Phe Ala Tyr Gly Thr
Ser Ser Asn Leu Pro Ser Ala 385 390 395 gtt tac aga aag agc gga acc
gtt gat tcc ttg gac gaa atc cca cca 2508Val Tyr Arg Lys Ser Gly Thr
Val Asp Ser Leu Asp Glu Ile Pro Pro 400 405 410 cag aac aac aat gtg
cca ccc agg caa gga ttc tcc cac agg ttg agc 2556Gln Asn Asn Asn Val
Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser 415 420 425 430 cac gtg
tcc atg ttc cgt tcc gga ttc agc aac agt tcc gtg agc atc 2604His Val
Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile 435 440 445
atc aga gct cct atg ttc tct tgg ata cac cgt agt gct gag ttc aac
2652Ile Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn
450 455 460 aac atc att gca tcc gac agc att act caa ata ccc ttg gtg
aaa gca 2700Asn Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Leu Val
Lys Ala 465 470 475 cat aca ctt cag tca ggt act act gtt gtc aga ggt
cca ggg ttt aca 2748His Thr Leu Gln Ser Gly Thr Thr Val Val Arg Gly
Pro Gly Phe Thr 480 485 490 gga gga gac att ctt cgt cgc aca agt gga
gga ccc ttt gct tac act 2796Gly Gly Asp Ile Leu Arg Arg Thr Ser Gly
Gly Pro Phe Ala Tyr Thr 495 500 505 510 att gtt aac atc aat ggc caa
ttg ccc caa agg tat cgt gca aga atc 2844Ile Val Asn Ile Asn Gly Gln
Leu Pro Gln Arg Tyr Arg Ala Arg Ile 515 520 525 cgc tat gcc tct act
aca aat ctc agg atc tac gtg act gtt gca ggt 2892Arg Tyr Ala Ser Thr
Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly 530 535 540 gaa agg atc
ttt gct ggt cag ttc aac aag act atg gat acc ggt gac 2940Glu Arg Ile
Phe Ala Gly Gln Phe Asn Lys Thr Met Asp Thr Gly Asp 545 550 555 cct
ttg aca ttc caa tct ttt agc tac gca act atc aac aca gct ttt 2988Pro
Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe 560 565
570 aca ttc cca atg agc cag agt agc ttc aca gta ggt gct gac act ttc
3036Thr Phe Pro Met Ser Gln Ser Ser Phe Thr Val Gly Ala Asp Thr Phe
575 580 585 590 agc tca ggg aat gaa gtt tac atc gac agg ttt gaa ttg
att cca gtt 3084Ser Ser Gly Asn Glu Val Tyr Ile Asp Arg Phe Glu Leu
Ile Pro Val 595 600 605 act gca acc ctc gag gct gag tac aac ctt gag
aga gcc cag aag gct 3132Thr Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu
Arg Ala Gln Lys Ala 610 615 620 gtg aac gcc ctc ttt acc tcc acc aat
cag ctt ggc ttg aaa act aac 3180Val Asn Ala Leu Phe Thr Ser Thr Asn
Gln Leu Gly Leu Lys Thr Asn 625 630 635 gtt act gac tat cac att gac
caa gtg tcc aac ttg gtc acc tac ctt 3228Val Thr Asp Tyr His Ile Asp
Gln Val Ser Asn Leu Val Thr Tyr Leu 640 645 650 agc gat gag ttc tgc
ctc gac gag aag cgt gaa ctc tcc gag aaa gtt 3276Ser Asp Glu Phe Cys
Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val 655 660 665 670 aaa cac
gcc aag cgt ctc agc gac gag agg aat ctc ttg caa gac tcc 3324Lys His
Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser 675 680 685
aac ttc aaa gac atc aac agg cag cca gaa cgt ggt tgg ggt gga agc
3372Asn Phe Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser
690 695 700 acc ggg atc acc atc caa gga ggc gac gat gtg ttc aag gag
aac tac 3420Thr Gly Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu
Asn Tyr 705 710 715 gtc acc ctc tcc gga act ttc gac gag tgc tac cct
acc tac ttg tac 3468Val Thr Leu Ser Gly Thr Phe Asp Glu Cys Tyr Pro
Thr Tyr Leu Tyr 720 725 730 cag aag atc gat gag tcc aaa ctc aaa gcc
ttc acc agg tat caa ctt 3516Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala
Phe Thr Arg Tyr Gln Leu 735 740 745 750 aga ggc tac atc gaa gac agc
caa gac ctt gaa atc tac tcg atc agg 3564Arg Gly Tyr Ile Glu Asp Ser
Gln Asp Leu Glu Ile Tyr Ser Ile Arg 755 760 765 tac aat gcc aag cac
gag acc gtg aat gtc cca ggt act ggt tcc ctc 3612Tyr Asn Ala Lys His
Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu 770 775 780 tgg cca ctt
tct gcc caa tct ccc att ggg aag tgt gga gag cct aac 3660Trp Pro Leu
Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn 785 790 795 aga
tgc gct cca cac ctt gag tgg aat cct gac ttg gac tgc tcc tgc 3708Arg
Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys 800 805
810 agg gat ggc gag aag tgt gcc cac cat tct cat cac ttc tcc ttg gac
3756Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp
815 820 825 830 atc gat gtg gga tgt act gac ctg aat gag gac ctc gga
gtc tgg gtc 3804Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly
Val Trp Val 835 840 845 atc ttc aag atc aag acc caa gac gga cac gca
aga ctt ggc aac ctt 3852Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala
Arg Leu Gly Asn Leu 850 855 860 gag ttt ctc gaa gag aaa cca ttg gtc
ggt gaa gct ctc gct cgt gtg 3900Glu Phe Leu Glu Glu Lys Pro Leu Val
Gly Glu Ala Leu Ala Arg Val 865 870 875 aag aga gca gag aag aag tgg
agg gac aaa cgt gag aaa ctc gaa tgg 3948Lys Arg Ala Glu Lys Lys Trp
Arg Asp Lys Arg Glu Lys Leu Glu Trp 880 885 890 gaa act aac atc gtt
tac aag gag gcc aaa gag tcc gtg gat gct ttg 3996Glu Thr Asn Ile Val
Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 895 900 905 910 ttc gtg
aac tcc caa tat gat cag ttg caa gcc gac acc aac atc gcc 4044Phe Val
Asn Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala 915 920 925
atg atc cac gcc gca gac aaa cgt gtg cac agc att cgt gag gct tac
4092Met Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr
930 935 940 ttg cct gag ttg tcc gtg atc cct ggt gtg aac gct gcc atc
ttc gag 4140Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile
Phe Glu 945 950 955 gaa ctt gag gga cgt atc ttt acc gca ttc tcc ttg
tac gat gcc aga 4188Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu
Tyr Asp Ala Arg 960 965 970 aac gtc atc aag aac ggt gac ttc aac aat
ggc ctc agc tgc tgg aat 4236Asn Val Ile Lys Asn Gly Asp Phe Asn Asn
Gly Leu Ser Cys Trp Asn 975 980 985 990 gtg aaa ggt cat gtg gac gtg
gag gaa cag aac aat cag cgt tcc gtc 4284Val Lys Gly His Val Asp Val
Glu Glu Gln Asn Asn Gln Arg Ser Val 995 1000 1005 ctg gtt gtg cct
gag tgg gaa gct gaa gtg tcc caa gag gtt aga 4329Leu Val Val Pro Glu
Trp Glu Ala Glu Val Ser Gln Glu Val Arg 1010 1015 1020 gtc tgt cca
ggt aga ggc tac att ctc cgt gtg acc gct tac aag 4374Val Cys Pro Gly
Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys 1025 1030 1035 gag gga
tac ggt gag ggt tgc gtg acc atc cac gag atc gag aac 4419Glu Gly Tyr
Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn 1040 1045 1050 aac
acc gac gag ctt aag ttc tcc aac tgc gtc gag gaa gaa atc 4464Asn Thr
Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu Glu Ile 1055 1060 1065
tat ccc aac aac acc gtt act tgc aac gac tac act gtg aat cag 4509Tyr
Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln 1070 1075
1080 gaa gag tac gga ggt gcc tac act agc cgt aac aga ggt tac aac
4554Glu Glu Tyr Gly Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn
1085 1090 1095 gaa gct cct tcc gtt cct gct gac tat gcc tcc gtg tac
gag gag 4599Glu Ala Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu
Glu 1100 1105 1110 aaa tcc tac aca gat ggc aga cgt gag aac cct tgc
gag ttc aac 4644Lys Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu
Phe Asn 1115 1120 1125 aga ggt tac agg gac tac aca cca ctt cca gtt
ggc tat gtt acc 4689Arg Gly Tyr Arg Asp Tyr Thr Pro Leu Pro Val Gly
Tyr Val Thr 1130 1135 1140 aag gag ctt gag tac ttt cct gag acc gac
aaa gtg tgg atc gag 4734Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys
Val Trp Ile Glu 1145 1150 1155 atc ggt gaa acc gag gga acc ttc atc
gtg gac agc gtg gag ctt 4779Ile Gly Glu Thr Glu Gly Thr Phe Ile Val
Asp Ser Val Glu Leu 1160 1165 1170 ctc ttg atg gag gaa taa
tgagatccac gatatcctgc aggaattggc 4827Leu Leu Met Glu Glu 1175
cggccagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
4887ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg
ggaaaactgt 4947ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt
ttttattcgg ttttcgctat 5007cgaactgtga aatggaaatg gatggagaag
agttaatgaa tgatatggtc cttttgttca 5067ttctcaaatt aatattattt
gttttttctc ttatttgttg tgtgttgaat ttgaaattat 5127aagagatatg
caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
5187cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
tcactaggca 5247acaaatatat tttcagacct agaaaagctg caaatgttac
tgaatacaag tatgtcctct 5307tgtgttttag acatttatgg actttccttt
atgtaatttt ccagaatcct tgtcagattc 5367taatcattgc tttataatta
tagttatact catggatttg tagttgagta tgaaaatatt 5427ttttaatgca
ttttatgact tgccaattga ttgacaacat gcatcaatcg acc
548061176PRTartificialSynthetic Construct 6Asp Asn Asn Pro Asn Ile
Asn Glu Cys Ile Pro Tyr Asn Cys Leu Ser 1 5 10 15 Asn Pro Glu Val
Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly Tyr 20 25 30 Thr Pro
Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser Glu 35 40 45
Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile Trp 50
55 60 Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
Glu 65 70 75 80 Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn
Gln Ala Ile 85 90 95 Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln
Ile Tyr Ala Glu Ser 100 105 110 Phe Arg Glu Trp Glu Ala Asp Pro Thr
Asn Pro Ala Leu Arg Glu Glu 115 120 125 Met Arg Ile Gln Phe Asn Asp
Met Asn Ser Ala Leu Thr Thr Ala Ile 130 135 140 Pro Leu Phe Ala Val
Gln Asn Tyr Gln Val Pro Leu Leu Ser Val Tyr 145 150 155 160 Val Gln
Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser Val 165 170 175
Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg Tyr 180
185 190 Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
Arg 195 200 205 Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp
Ser Arg Asp 210 215 220 Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu
Thr Leu Thr Val Leu 225 230 235 240 Asp Ile Val Ser Leu Phe Pro Asn
Tyr Asp Ser Arg Thr Tyr Pro Ile 245 250 255 Arg Thr Val Ser Gln Leu
Thr Arg Glu Ile Tyr Thr Asn Pro Val Leu 260 265 270 Glu Asn Phe Asp
Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu Gly 275 280 285 Ser Ile
Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr Ile 290 295 300
Tyr Thr Asp Ala His
Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln Ile 305 310 315 320 Met Ala
Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro Leu 325 330 335
Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala Gln 340
345 350 Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
Arg 355 360 365 Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val
Leu Asp Gly 370 375 380 Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu
Pro Ser Ala Val Tyr 385 390 395 400 Arg Lys Ser Gly Thr Val Asp Ser
Leu Asp Glu Ile Pro Pro Gln Asn 405 410 415 Asn Asn Val Pro Pro Arg
Gln Gly Phe Ser His Arg Leu Ser His Val 420 425 430 Ser Met Phe Arg
Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile Arg 435 440 445 Ala Pro
Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn Ile 450 455 460
Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His Thr 465
470 475 480 Leu Gln Ser Gly Thr Thr Val Val Arg Gly Pro Gly Phe Thr
Gly Gly 485 490 495 Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala
Tyr Thr Ile Val 500 505 510 Asn Ile Asn Gly Gln Leu Pro Gln Arg Tyr
Arg Ala Arg Ile Arg Tyr 515 520 525 Ala Ser Thr Thr Asn Leu Arg Ile
Tyr Val Thr Val Ala Gly Glu Arg 530 535 540 Ile Phe Ala Gly Gln Phe
Asn Lys Thr Met Asp Thr Gly Asp Pro Leu 545 550 555 560 Thr Phe Gln
Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe 565 570 575 Pro
Met Ser Gln Ser Ser Phe Thr Val Gly Ala Asp Thr Phe Ser Ser 580 585
590 Gly Asn Glu Val Tyr Ile Asp Arg Phe Glu Leu Ile Pro Val Thr Ala
595 600 605 Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala Gln Lys Ala
Val Asn 610 615 620 Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys
Thr Asn Val Thr 625 630 635 640 Asp Tyr His Ile Asp Gln Val Ser Asn
Leu Val Thr Tyr Leu Ser Asp 645 650 655 Glu Phe Cys Leu Asp Glu Lys
Arg Glu Leu Ser Glu Lys Val Lys His 660 665 670 Ala Lys Arg Leu Ser
Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn Phe 675 680 685 Lys Asp Ile
Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser Thr Gly 690 695 700 Ile
Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr 705 710
715 720 Leu Ser Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
Lys 725 730 735 Ile Asp Glu Ser Lys Leu Lys Ala Phe Thr Arg Tyr Gln
Leu Arg Gly 740 745 750 Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr
Ser Ile Arg Tyr Asn 755 760 765 Ala Lys His Glu Thr Val Asn Val Pro
Gly Thr Gly Ser Leu Trp Pro 770 775 780 Leu Ser Ala Gln Ser Pro Ile
Gly Lys Cys Gly Glu Pro Asn Arg Cys 785 790 795 800 Ala Pro His Leu
Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp 805 810 815 Gly Glu
Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp 820 825 830
Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe 835
840 845 Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu
Phe 850 855 860 Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg
Val Lys Arg 865 870 875 880 Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu
Lys Leu Glu Trp Glu Thr 885 890 895 Asn Ile Val Tyr Lys Glu Ala Lys
Glu Ser Val Asp Ala Leu Phe Val 900 905 910 Asn Ser Gln Tyr Asp Gln
Leu Gln Ala Asp Thr Asn Ile Ala Met Ile 915 920 925 His Ala Ala Asp
Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro 930 935 940 Glu Leu
Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu 945 950 955
960 Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val
965 970 975 Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn
Val Lys 980 985 990 Gly His Val Asp Val Glu Glu Gln Asn Asn Gln Arg
Ser Val Leu Val 995 1000 1005 Val Pro Glu Trp Glu Ala Glu Val Ser
Gln Glu Val Arg Val Cys 1010 1015 1020 Pro Gly Arg Gly Tyr Ile Leu
Arg Val Thr Ala Tyr Lys Glu Gly 1025 1030 1035 Tyr Gly Glu Gly Cys
Val Thr Ile His Glu Ile Glu Asn Asn Thr 1040 1045 1050 Asp Glu Leu
Lys Phe Ser Asn Cys Val Glu Glu Glu Ile Tyr Pro 1055 1060 1065 Asn
Asn Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln Glu Glu 1070 1075
1080 Tyr Gly Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn Glu Ala
1085 1090 1095 Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu
Lys Ser 1100 1105 1110 Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu
Phe Asn Arg Gly 1115 1120 1125 Tyr Arg Asp Tyr Thr Pro Leu Pro Val
Gly Tyr Val Thr Lys Glu 1130 1135 1140 Leu Glu Tyr Phe Pro Glu Thr
Asp Lys Val Trp Ile Glu Ile Gly 1145 1150 1155 Glu Thr Glu Gly Thr
Phe Ile Val Asp Ser Val Glu Leu Leu Leu 1160 1165 1170 Met Glu Glu
1175 74990DNAartificialexpression cassette encoding Cry1A.105 amino
acid sequence 7ggtccgatgt gagacttttc aacaaagggt aatatccgga
aacctcctcg gattccattg 60cccagctatc tgtcacttta ttgtgaagat agtggaaaag
gaaggtggct cctacaaatg 120ccatcattgc gataaaggaa aggccatcgt
tgaagatgcc tctgccgaca gtggtcccaa 180agatggaccc ccacccacga
ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 240aaagcaagtg
gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg
300gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
atagtggaaa 360aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
aaaggccatc gttgaagatg 420cctctgccga cagtggtccc aaagatggac
ccccacccac gaggagcatc gtggaaaaag 480aagacgttcc aaccacgtct
tcaaagcaag tggattgatg tgatatctcc actgacgtaa 540gggatgacgc
acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
600ttcatttgga gaggacacgc tgacaagctg actctagcag atcctctaga
accatcttcc 660acacactcaa gccacactat tggagaacac acagggacaa
cacaccataa gatccaaggg 720aggcctccgc cgccgccggt aaccaccccg
cccctctcct ctttctttct ccgttttttt 780ttccgtctcg gtctcgatct
ttggccttgg tagtttgggt gggcgagagg cggcttcgtg 840cgcgcccaga
tcggtgcgcg ggaggggcgg gatctcgcgg ctggggctct cgccggcgtg
900gatccggccc ggatctcgcg gggaatgggg ctctcggatg tagatctgcg
atccgccgtt 960gttgggggag atgatggggg gtttaaaatt tccgccgtgc
taaacaagat caggaagagg 1020ggaaaagggc actatggttt atatttttat
atatttctgc tgcttcgtca ggcttagatg 1080tgctagatct ttctttcttc
tttttgtggg tagaatttga atccctcagc attgttcatc 1140ggtagttttt
cttttcatga tttgtgacaa atgcagcctc gtgcggagct tttttgtagg
1200tagaagtgat caacc atg gac aac aac cca aac atc aac gag tgc atc
ccg 1251 Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro 1 5 10 tac
aac tgc ctc agc aac cct gag gtc gag gtg ctc ggc ggt gag cgc 1299Tyr
Asn Cys Leu Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg 15 20
25 atc gag acc ggt tac acc ccc atc gac atc tcc ctc tcc ctc acg cag
1347Ile Glu Thr Gly Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln
30 35 40 ttc ctg ctc agc gag ttc gtg cca ggc gct ggc ttc gtc ctg
ggc ctc 1395Phe Leu Leu Ser Glu Phe Val Pro Gly Ala Gly Phe Val Leu
Gly Leu 45 50 55 60 gtg gac atc atc tgg ggc atc ttt ggc ccc tcc cag
tgg gac gcc ttc 1443Val Asp Ile Ile Trp Gly Ile Phe Gly Pro Ser Gln
Trp Asp Ala Phe 65 70 75 ctg gtg caa atc gag cag ctc atc aac cag
agg atc gag gag ttc gcc 1491Leu Val Gln Ile Glu Gln Leu Ile Asn Gln
Arg Ile Glu Glu Phe Ala 80 85 90 agg aac cag gcc atc agc cgc ctg
gag ggc ctc agc aac ctc tac caa 1539Arg Asn Gln Ala Ile Ser Arg Leu
Glu Gly Leu Ser Asn Leu Tyr Gln 95 100 105 atc tac gct gag agc ttc
cgc gag tgg gag gcc gac ccc act aac cca 1587Ile Tyr Ala Glu Ser Phe
Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro 110 115 120 gct ctc cgc gag
gag atg cgc atc cag ttc aac gac atg aac agc gcc 1635Ala Leu Arg Glu
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala 125 130 135 140 ctg
acc acc gcc atc cca ctc ttc gcc gtc cag aac tac caa gtc ccg 1683Leu
Thr Thr Ala Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro 145 150
155 ctc ctg tcc gtg tac gtc cag gcc gcc aac ctg cac ctc agc gtg ctg
1731Leu Leu Ser Val Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu
160 165 170 agg gac gtc agc gtg ttt ggc cag agg tgg ggc ttc gac gcc
gcc acc 1779Arg Asp Val Ser Val Phe Gly Gln Arg Trp Gly Phe Asp Ala
Ala Thr 175 180 185 atc aac agc cgc tac aac gac ctc acc agg ctg atc
ggc aac tac acc 1827Ile Asn Ser Arg Tyr Asn Asp Leu Thr Arg Leu Ile
Gly Asn Tyr Thr 190 195 200 gac cac gct gtc cgc tgg tac aac act ggc
ctg gag cgc gtc tgg ggc 1875Asp His Ala Val Arg Trp Tyr Asn Thr Gly
Leu Glu Arg Val Trp Gly 205 210 215 220 cct gat tct aga gac tgg att
cgc tac aac cag ttc agg cgc gag ctg 1923Pro Asp Ser Arg Asp Trp Ile
Arg Tyr Asn Gln Phe Arg Arg Glu Leu 225 230 235 acc ctc acc gtc ctg
gac att gtg tcc ctc ttc ccg aac tac gac tcc 1971Thr Leu Thr Val Leu
Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser 240 245 250 cgc acc tac
ccg atc cgc acc gtg tcc caa ctg acc cgc gaa atc tac 2019Arg Thr Tyr
Pro Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr 255 260 265 acc
aac ccc gtc ctg gag aac ttc gac ggt agc ttc agg ggc agc gcc 2067Thr
Asn Pro Val Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala 270 275
280 cag ggc atc gag ggc tcc atc agg agc cca cac ctg atg gac atc ctc
2115Gln Gly Ile Glu Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu
285 290 295 300 aac agc atc act atc tac acc gat gcc cac cgc ggc gag
tac tac tgg 2163Asn Ser Ile Thr Ile Tyr Thr Asp Ala His Arg Gly Glu
Tyr Tyr Trp 305 310 315 tcc ggc cac cag atc atg gcc tcc ccg gtc ggc
ttc agc ggc ccc gag 2211Ser Gly His Gln Ile Met Ala Ser Pro Val Gly
Phe Ser Gly Pro Glu 320 325 330 ttt acc ttt cct ctc tac ggc acg atg
ggc aac gcc gct cca caa caa 2259Phe Thr Phe Pro Leu Tyr Gly Thr Met
Gly Asn Ala Ala Pro Gln Gln 335 340 345 cgc atc gtc gct cag ctg ggc
cag ggc gtc tac cgc acc ctg agc tcc 2307Arg Ile Val Ala Gln Leu Gly
Gln Gly Val Tyr Arg Thr Leu Ser Ser 350 355 360 acc ctg tac cgc agg
ccc ttc aac atc ggt atc aac aac cag cag ctg 2355Thr Leu Tyr Arg Arg
Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu 365 370 375 380 tcc gtc
ctg gat ggc act gag ttc gcc tac ggc acc tcc tcc aac ctg 2403Ser Val
Leu Asp Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu 385 390 395
ccc tcc gct gtc tac cgc aag agc ggc acg gtg gat tcc ctg gac gag
2451Pro Ser Ala Val Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu
400 405 410 atc cca cca cag aac aac aat gtg ccc ccc agg cag ggt ttt
tcc cac 2499Ile Pro Pro Gln Asn Asn Asn Val Pro Pro Arg Gln Gly Phe
Ser His 415 420 425 agg ctc agc cac gtg tcc atg ttc cgc tcc ggc ttc
agc aac tcg tcc 2547Arg Leu Ser His Val Ser Met Phe Arg Ser Gly Phe
Ser Asn Ser Ser 430 435 440 gtg agc atc atc aga gct cct atg ttc tct
tgg ata cac cgt agt gct 2595Val Ser Ile Ile Arg Ala Pro Met Phe Ser
Trp Ile His Arg Ser Ala 445 450 455 460 gag ttc aac aac atc att gca
tcc gac agc att act caa ata ccc ttg 2643Glu Phe Asn Asn Ile Ile Ala
Ser Asp Ser Ile Thr Gln Ile Pro Leu 465 470 475 gtg aaa gca cat aca
ctt cag tca ggt act act gtt gtc aga ggt cca 2691Val Lys Ala His Thr
Leu Gln Ser Gly Thr Thr Val Val Arg Gly Pro 480 485 490 ggg ttt aca
gga gga gac att ctt cgt cgc aca agt gga gga ccc ttt 2739Gly Phe Thr
Gly Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe 495 500 505 gct
tac act att gtt aac atc aat ggc caa ttg ccc caa agg tat cgt 2787Ala
Tyr Thr Ile Val Asn Ile Asn Gly Gln Leu Pro Gln Arg Tyr Arg 510 515
520 gca aga atc cgc tat gcc tct act aca aat ctc agg atc tac gtg act
2835Ala Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr
525 530 535 540 gtt gca ggt gaa agg atc ttt gct ggt cag ttc aac aag
act atg gat 2883Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe Asn Lys
Thr Met Asp 545 550 555 acc ggt gac cct ttg aca ttc caa tct ttt agc
tac gca act atc aac 2931Thr Gly Asp Pro Leu Thr Phe Gln Ser Phe Ser
Tyr Ala Thr Ile Asn 560 565 570 aca gct ttt aca ttc cca atg agc cag
agt agc ttc aca gta ggt gct 2979Thr Ala Phe Thr Phe Pro Met Ser Gln
Ser Ser Phe Thr Val Gly Ala 575 580 585 gac act ttc agc tca ggg aat
gaa gtt tac atc gac agg ttt gaa ttg 3027Asp Thr Phe Ser Ser Gly Asn
Glu Val Tyr Ile Asp Arg Phe Glu Leu 590 595 600 att cca gtt act gca
acc ctc gag gct gag tac aac ctt gag aga gcc 3075Ile Pro Val Thr Ala
Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala 605 610 615 620 cag aag
gct gtg aac gcc ctc ttt acc tcc acc aat cag ctt ggc ttg 3123Gln Lys
Ala Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu 625 630 635
aaa act aac gtt act gac tat cac att gac caa gtg tcc aac ttg gtc
3171Lys Thr Asn Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val
640 645 650 acc tac ctt agc gat gag ttc tgc ctc gac gag aag cgt gaa
ctc tcc 3219Thr Tyr Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu
Leu Ser 655 660 665 gag aaa gtt aaa cac gcc aag cgt ctc agc gac gag
agg aat ctc ttg 3267Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp Glu
Arg Asn Leu Leu 670 675 680 caa gac tcc aac ttc aaa gac atc aac agg
cag cca gaa cgt ggt tgg 3315Gln Asp Ser Asn Phe Lys Asp Ile Asn Arg
Gln Pro Glu Arg Gly Trp 685 690 695 700 ggt gga agc acc ggg atc acc
atc caa gga ggc gac gat gtg ttc aag 3363Gly Gly Ser Thr Gly Ile Thr
Ile Gln Gly Gly Asp Asp Val Phe Lys
705 710 715 gag aac tac gtc acc ctc tcc gga act ttc gac gag tgc tac
cct acc 3411Glu Asn Tyr Val Thr Leu Ser Gly Thr Phe Asp Glu Cys Tyr
Pro Thr 720 725 730 tac ttg tac cag aag atc gat gag tcc aaa ctc aaa
gcc ttc acc agg 3459Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys
Ala Phe Thr Arg 735 740 745 tat caa ctt aga ggc tac atc gaa gac agc
caa gac ctt gaa atc tac 3507Tyr Gln Leu Arg Gly Tyr Ile Glu Asp Ser
Gln Asp Leu Glu Ile Tyr 750 755 760 tcg atc agg tac aat gcc aag cac
gag acc gtg aat gtc cca ggt act 3555Ser Ile Arg Tyr Asn Ala Lys His
Glu Thr Val Asn Val Pro Gly Thr 765 770 775 780 ggt tcc ctc tgg cca
ctt tct gcc caa tct ccc att ggg aag tgt gga 3603Gly Ser Leu Trp Pro
Leu Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly 785 790 795 gag cct aac
aga tgc gct cca cac ctt gag tgg aat cct gac ttg gac 3651Glu Pro Asn
Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp 800 805 810 tgc
tcc tgc agg gat ggc gag aag tgt gcc cac cat tct cat cac ttc 3699Cys
Ser Cys Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe 815 820
825 tcc ttg gac atc gat gtg gga tgt act gac ctg aat gag gac ctc gga
3747Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly
830 835 840 gtc tgg gtc atc ttc aag atc aag acc caa gac gga cac gca
aga ctt 3795Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala
Arg Leu 845 850 855 860 ggc aac ctt gag ttt ctc gaa gag aaa cca ttg
gtc ggt gaa gct ctc 3843Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu
Val Gly Glu Ala Leu 865 870 875 gct cgt gtg aag aga gca gag aag aag
tgg agg gac aaa cgt gag aaa 3891Ala Arg Val Lys Arg Ala Glu Lys Lys
Trp Arg Asp Lys Arg Glu Lys 880 885 890 ctc gaa tgg gaa act aac atc
gtt tac aag gag gcc aaa gag tcc gtg 3939Leu Glu Trp Glu Thr Asn Ile
Val Tyr Lys Glu Ala Lys Glu Ser Val 895 900 905 gat gct ttg ttc gtg
aac tcc caa tat gat cag ttg caa gcc gac acc 3987Asp Ala Leu Phe Val
Asn Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr 910 915 920 aac atc gcc
atg atc cac gcc gca gac aaa cgt gtg cac agc att cgt 4035Asn Ile Ala
Met Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg 925 930 935 940
gag gct tac ttg cct gag ttg tcc gtg atc cct ggt gtg aac gct gcc
4083Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala
945 950 955 atc ttc gag gaa ctt gag gga cgt atc ttt acc gca ttc tcc
ttg tac 4131Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser
Leu Tyr 960 965 970 gat gcc aga aac gtc atc aag aac ggt gac ttc aac
aat ggc ctc agc 4179Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn
Asn Gly Leu Ser 975 980 985 tgc tgg aat gtg aaa ggt cat gtg gac gtg
gag gaa cag aac aat cag 4227Cys Trp Asn Val Lys Gly His Val Asp Val
Glu Glu Gln Asn Asn Gln 990 995 1000 cgt tcc gtc ctg gtt gtg cct
gag tgg gaa gct gaa gtg tcc caa 4272Arg Ser Val Leu Val Val Pro Glu
Trp Glu Ala Glu Val Ser Gln 1005 1010 1015 gag gtt aga gtc tgt cca
ggt aga ggc tac att ctc cgt gtg acc 4317Glu Val Arg Val Cys Pro Gly
Arg Gly Tyr Ile Leu Arg Val Thr 1020 1025 1030 gct tac aag gag gga
tac ggt gag ggt tgc gtg acc atc cac gag 4362Ala Tyr Lys Glu Gly Tyr
Gly Glu Gly Cys Val Thr Ile His Glu 1035 1040 1045 atc gag aac aac
acc gac gag ctt aag ttc tcc aac tgc gtc gag 4407Ile Glu Asn Asn Thr
Asp Glu Leu Lys Phe Ser Asn Cys Val Glu 1050 1055 1060 gaa gaa atc
tat ccc aac aac acc gtt act tgc aac gac tac act 4452Glu Glu Ile Tyr
Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr 1065 1070 1075 gtg aat
cag gaa gag tac gga ggt gcc tac act agc cgt aac aga 4497Val Asn Gln
Glu Glu Tyr Gly Gly Ala Tyr Thr Ser Arg Asn Arg 1080 1085 1090 ggt
tac aac gaa gct cct tcc gtt cct gct gac tat gcc tcc gtg 4542Gly Tyr
Asn Glu Ala Pro Ser Val Pro Ala Asp Tyr Ala Ser Val 1095 1100 1105
tac gag gag aaa tcc tac aca gat ggc aga cgt gag aac cct tgc 4587Tyr
Glu Glu Lys Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys 1110 1115
1120 gag ttc aac aga ggt tac agg gac tac aca cca ctt cca gtt ggc
4632Glu Phe Asn Arg Gly Tyr Arg Asp Tyr Thr Pro Leu Pro Val Gly
1125 1130 1135 tat gtt acc aag gag ctt gag tac ttt cct gag acc gac
aaa gtg 4677Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys
Val 1140 1145 1150 tgg atc gag atc ggt gaa acc gag gga acc ttc atc
gtg gac agc 4722Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val
Asp Ser 1155 1160 1165 gtg gag ctt ctc ttg atg gag gaa taa tga
gatctatcga ttctagaagg 4772Val Glu Leu Leu Leu Met Glu Glu 1170 1175
cctgaattct gcatgcgttt ggacgtatgc tcattcaggt tggagccaat ttggttgatg
4832tgtgtgcgag ttcttgcgag tctgatgaga catctctgta ttgtgtttct
ttccccagtg 4892ttttctgtac ttgtgtaatc ggctaatcgc caacagattc
ggcgatgaat aaatgagaaa 4952taaattgttc tgattttgag tgcaaaaaaa aaggaatt
499081177PRTartificialSynthetic Construct 8Met Asp Asn Asn Pro Asn
Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 Ser Asn Pro Glu
Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 Tyr Thr
Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50
55 60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln
Ile 65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg
Asn Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr
Gln Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu Trp Glu Ala Asp Pro
Thr Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met Arg Ile Gln Phe Asn
Asp Met Asn Ser Ala Leu Thr Thr Ala 130 135 140 Ile Pro Leu Phe Ala
Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 Tyr Val
Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180
185 190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala
Val 195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro
Asp Ser Arg 210 215 220 Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu
Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Val Ser Leu Phe Pro
Asn Tyr Asp Ser Arg Thr Tyr Pro 245 250 255 Ile Arg Thr Val Ser Gln
Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265 270 Leu Glu Asn Phe
Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 Gly Ser
Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 305
310 315 320 Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr
Phe Pro 325 330 335 Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln
Arg Ile Val Ala 340 345 350 Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu
Ser Ser Thr Leu Tyr Arg 355 360 365 Arg Pro Phe Asn Ile Gly Ile Asn
Asn Gln Gln Leu Ser Val Leu Asp 370 375 380 Gly Thr Glu Phe Ala Tyr
Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400 Tyr Arg Lys
Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 Asn
Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425
430 Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445 Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe
Asn Asn 450 455 460 Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Leu
Val Lys Ala His 465 470 475 480 Thr Leu Gln Ser Gly Thr Thr Val Val
Arg Gly Pro Gly Phe Thr Gly 485 490 495 Gly Asp Ile Leu Arg Arg Thr
Ser Gly Gly Pro Phe Ala Tyr Thr Ile 500 505 510 Val Asn Ile Asn Gly
Gln Leu Pro Gln Arg Tyr Arg Ala Arg Ile Arg 515 520 525 Tyr Ala Ser
Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu 530 535 540 Arg
Ile Phe Ala Gly Gln Phe Asn Lys Thr Met Asp Thr Gly Asp Pro 545 550
555 560 Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe
Thr 565 570 575 Phe Pro Met Ser Gln Ser Ser Phe Thr Val Gly Ala Asp
Thr Phe Ser 580 585 590 Ser Gly Asn Glu Val Tyr Ile Asp Arg Phe Glu
Leu Ile Pro Val Thr 595 600 605 Ala Thr Leu Glu Ala Glu Tyr Asn Leu
Glu Arg Ala Gln Lys Ala Val 610 615 620 Asn Ala Leu Phe Thr Ser Thr
Asn Gln Leu Gly Leu Lys Thr Asn Val 625 630 635 640 Thr Asp Tyr His
Ile Asp Gln Val Ser Asn Leu Val Thr Tyr Leu Ser 645 650 655 Asp Glu
Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn 675
680 685 Phe Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser
Thr 690 695 700 Gly Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu
Asn Tyr Val 705 710 715 720 Thr Leu Ser Gly Thr Phe Asp Glu Cys Tyr
Pro Thr Tyr Leu Tyr Gln 725 730 735 Lys Ile Asp Glu Ser Lys Leu Lys
Ala Phe Thr Arg Tyr Gln Leu Arg 740 745 750 Gly Tyr Ile Glu Asp Ser
Gln Asp Leu Glu Ile Tyr Ser Ile Arg Tyr 755 760 765 Asn Ala Lys His
Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 770 775 780 Pro Leu
Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn Arg 785 790 795
800 Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg
805 810 815 Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu
Asp Ile 820 825 830 Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly
Val Trp Val Ile 835 840 845 Phe Lys Ile Lys Thr Gln Asp Gly His Ala
Arg Leu Gly Asn Leu Glu 850 855 860 Phe Leu Glu Glu Lys Pro Leu Val
Gly Glu Ala Leu Ala Arg Val Lys 865 870 875 880 Arg Ala Glu Lys Lys
Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu 885 890 895 Thr Asn Ile
Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 900 905 910 Val
Asn Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala Met 915 920
925 Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu
930 935 940 Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe
Glu Glu 945 950 955 960 Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu
Tyr Asp Ala Arg Asn 965 970 975 Val Ile Lys Asn Gly Asp Phe Asn Asn
Gly Leu Ser Cys Trp Asn Val 980 985 990 Lys Gly His Val Asp Val Glu
Glu Gln Asn Asn Gln Arg Ser Val Leu 995 1000 1005 Val Val Pro Glu
Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val 1010 1015 1020 Cys Pro
Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu 1025 1030 1035
Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn 1040
1045 1050 Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu Glu Ile
Tyr 1055 1060 1065 Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Val
Asn Gln Glu 1070 1075 1080 Glu Tyr Gly Gly Ala Tyr Thr Ser Arg Asn
Arg Gly Tyr Asn Glu 1085 1090 1095 Ala Pro Ser Val Pro Ala Asp Tyr
Ala Ser Val Tyr Glu Glu Lys 1100 1105 1110 Ser Tyr Thr Asp Gly Arg
Arg Glu Asn Pro Cys Glu Phe Asn Arg 1115 1120 1125 Gly Tyr Arg Asp
Tyr Thr Pro Leu Pro Val Gly Tyr Val Thr Lys 1130 1135 1140 Glu Leu
Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile Glu Ile 1145 1150 1155
Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu 1160
1165 1170 Leu Met Glu Glu 1175
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