U.S. patent application number 13/631560 was filed with the patent office on 2013-04-04 for histamine-releasing factor (hrf), hrf-receptor and methods of modulating inflammation.
This patent application is currently assigned to LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY. The applicant listed for this patent is LA JOLLA INSTITUTE FOR ALLERGY AND IMMU. Invention is credited to Toshiaki Kawakami, Yuko Kawakami.
Application Number | 20130084293 13/631560 |
Document ID | / |
Family ID | 44712849 |
Filed Date | 2013-04-04 |
United States Patent
Application |
20130084293 |
Kind Code |
A1 |
Kawakami; Toshiaki ; et
al. |
April 4, 2013 |
HISTAMINE-RELEASING FACTOR (HRF), HRF-RECEPTOR AND METHODS OF
MODULATING INFLAMMATION
Abstract
Methods of treating a food allergy, allergic reactions,
hypersensitivity, inflammatory responses, inflammation are
provided. In one method, histamine releasing factor
(HRF)/translationally controlled tumor protein (TCTP) is contacted
with a compound that inhibits or reduces binding of HRF/TCTP to an
immunoglobulin in order to treat the food allergy, allergic
reaction, hypersensitivity, inflammatory response, or inflammation.
Methods of reducing or decreasing the probability, severity,
frequency, duration or preventing a subject from having an acute or
chronic food allergy, allergic reaction, hypersensitivity, an
inflammatory response or inflammation, are also provided. In one
method, histamine releasing factor (HRF)/translationally controlled
tumor protein (TCTP) is contacted with a compound that inhibits or
reduces binding of HRF/TCTP to an immunoglobulin in order to reduce
or decrease the probability, severity, frequency, duration or
prevent a subject from having an acute or chronic food allergy,
allergic reaction, hypersensitivity, an inflammatory response or
inflammation.
Inventors: |
Kawakami; Toshiaki; (Del
Mar, CA) ; Kawakami; Yuko; (Del Mar, CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
LA JOLLA INSTITUTE FOR ALLERGY AND IMMU; |
La Jolla |
CA |
US |
|
|
Assignee: |
LA JOLLA INSTITUTE FOR ALLERGY AND
IMMUNOLOGY
La Jolla
CA
|
Family ID: |
44712849 |
Appl. No.: |
13/631560 |
Filed: |
September 28, 2012 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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PCT/US2011/030809 |
Mar 31, 2011 |
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13631560 |
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61326079 |
Apr 20, 2010 |
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61319652 |
Mar 31, 2010 |
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Current U.S.
Class: |
424/135.1 ;
424/133.1; 424/136.1; 424/152.1; 424/172.1; 530/389.1 |
Current CPC
Class: |
A61K 38/1709 20130101;
C07K 2317/76 20130101; A61K 39/3955 20130101; C07K 14/47 20130101;
C07K 14/52 20130101; C07K 2319/00 20130101; G01N 2800/24 20130101;
C07K 7/08 20130101; A61K 2039/505 20130101; G01N 2500/02 20130101;
C07K 16/18 20130101; C07K 2317/24 20130101 |
Class at
Publication: |
424/135.1 ;
424/172.1; 424/133.1; 424/152.1; 424/136.1; 530/389.1 |
International
Class: |
A61K 39/395 20060101
A61K039/395; C07K 16/18 20060101 C07K016/18 |
Goverment Interests
GOVERNMENT SUPPORT
[0002] This work was supported in part by Grant AI050209 from the
National Institutes of Health. The government has certain rights in
the invention.
Claims
1. (canceled)
2. A method of treating an allergic reaction, hypersensitivity, an
inflammatory response or inflammation, comprising contacting
histamine releasing factor (HRF)/translationally controlled tumor
protein (TCTP) with a compound that inhibits or reduces binding of
HRF/TCTP to an immunoglobulin thereby treating the allergic
reaction, hypersensitivity, inflammatory response or
inflammation.
3. (canceled)
4. The method of claim 2, wherein the compound comprises a peptide
or polypeptide.
5. The method of claim 4, wherein the peptide or polypeptide
comprises an antibody or an antibody subsequence that binds to
HRF/TCTP.
6. The method of claim 5, wherein the antibody or antibody
subsequence is human or humanized.
7. The method of claim 5, wherein the antibody or antibody
subsequence is monoclonal.
8. The method of claim 5, wherein the antibody or antibody
subsequence competes for binding of an antibody set forth in Tables
2 or 3 for binding to the HRF/TCTP.
9. The method of claim 5, wherein the antibody or antibody
subsequence binds to an epitope of HRF/TCTP to which an antibody
set forth in Tables 2 or 3 binds.
10. The method of claim 5, wherein the antibody or antibody
subsequence has a binding affinity (Kd) for binding to HRF/TCTP of
about 10.sup.-5 to about 10.sup.-13 M.
11.-12. (canceled)
13. The method of claim 12, wherein the IgG or IgA isotype is
selected from IgG1, IgG2, IgG3, and IgG4; and IgA1 and IgA2.
14. The method of claim 5, wherein the antibody subsequence is
selected from Fab, Fab', F(ab').sub.2, Fv, Fd, single-chain Fv
(scFv), disulfide-linked Fvs (sdFv), light chain variable region
V.sub.L, heavy chain variable region V.sub.H, trispecific
(Fab.sub.3), bispecific (Fab.sub.2), diabody
((V.sub.L-V.sub.H).sub.2 or (V.sub.H-V.sub.L).sub.2), triabody
(trivalent), tetrabody (tetravalent), minibody
((scF.sub.V-C.sub.H).sub.2), bispecific single-chain Fv (Bis-scFv),
IgGdeltaCH.sub.2, scFv-Fc, (scFv).sub.2-Fc and IgG4PE.
15. The method of claim 4, wherein the HRF/TCTP polypeptide, or a
fragment of an HRF/TCTP polypeptide comprises a fusion or chimeric
polypeptide.
16.-17. (canceled)
18. The method of claim 4, wherein the peptide or polypeptide
comprises an HRF/TCTP polypeptide, or a fragment of an HRF/TCTP
polypeptide that binds to an immunoglobulin.
19. The method of claim 15 or 18, wherein the fragment of HRF/TCTP
polypeptide that binds to an immunoglobulin comprises or consists
of amino acids 1-19 or amino acids 79-142 of a mammalian HRH/TCTP
sequence.
20.-22. (canceled)
23. The method of claim 2, wherein the food allergy, allergic
reaction, hypersensitivity, inflammatory response or inflammation
is chronic or acute.
24. (canceled)
25. The method of claim 2, wherein the treatment is sufficient to
decrease, reduce, inhibit, suppress, limit, control or improve the
probability, severity, frequency, or duration of one or more
adverse symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with the food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation.
26. The method of claim 2, wherein the method reduces or inhibits
progression, severity, frequency, duration or probability of an
adverse symptom of the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation.
27. (canceled)
28. The method of claim 2, wherein the allergic reaction is
selected from: Extrinsic or intrinsic bronchial asthma; Allergic
rhinitis; Onchocercal dermatitis; Atopic dermatitis; eczema; rash;
allergic urticaria (e.g. hives); allergic conjunctivitis; Drug
reactions; Nodules, eosinophilia, rheumatism, dermatitis, and
swelling (NERDS); Eosophageal and a gastrointestinal allergy.
29. The method of claim 2, wherein the hypersensitivity,
inflammatory response or inflammation comprises a respiratory
disease or disorder.
30. (canceled)
31. The method of claim 29, wherein the respiratory disease or
disorder comprises asthma, allergic asthma, bronchiolitis or
pleuritis.
32. The method of claim 29, wherein the respiratory disease or
disorder is selected from: Airway Obstruction, Apnea, Asbestosis,
Atelectasis, Berylliosis, Bronchiectasis, Bronchiolitis,
Bronchiolitis Obliterans Organizing Pneumonia, Bronchitis,
Bronchopulmonary Dysplasia, Empyema, Pleural Empyema, Pleural
Epiglottitis, Hemoptysis, Hypertension, Kartagener Syndrome,
Meconium Aspiration, Pleural Effusion, Pleurisy, Pneumonia,
Pneumothorax, Respiratory Distress Syndrome, Respiratory
Hypersensitivity, Rhinoscleroma, Scimitar Syndrome, Severe Acute
Respiratory Syndrome, Silicosis, Tracheal Stenosis, eosinophilic
pleural effusions, Histiocytosis; chronic eosinophilic pneumonia;
hypersensitivity pneumonitis; Allergic bronchopulmonary
aspergillosis; Sarcoidosis; Idiopathic pulmonary fibrosis;
pulmonary edema; pulmonary embolism; pulmonary emphysema; Pulmonary
Hyperventilation; Pulmonary Alveolar Proteinosis; Chronic
Obstructive Pulmonary Disease (COPD); Interstitial Lung Disease;
allergic rhinoconjunctivitis; allergic conjunctivitis and Topical
eosinophilia.
33. The method of claim 2, wherein the allergic reaction,
hypersensitivity, inflammatory response or inflammation affects the
skin or eye.
34. A method of increasing, enhancing or stimulating
airway-dilation, comprising administering to a subject in need of
increasing airway-dilation an amount of a compound that inhibits or
reduces binding of HRF/TCTP to an immunoglobulin sufficient to
increase, enhance or stimulate airway-dilation in the subject.
35. (canceled)
36. The method of claim 1 or 34, wherein the compound is
administered prior to, substantially contemporaneously with or
following one or more adverse symptoms, disorders, illnesses,
pathologies, diseases, or complications caused by or associated
with the food allergy, allergic reaction or hypersensitivity.
37. The method of claim 1 or 34, further comprising administering a
second drug.
38.-39. (canceled)
40. The method of claim 1 or 34, wherein the compound is
administered via ingestion, via inhalation, or topically.
41.-62. (canceled)
63. An isolated or purified antibody or antibody subsequence that
binds to mammalian HRF/TCTP sequence comprising or consisting of
amino acids 1-19 (MIIYRDLISHDEMFSDIYK, SEQ ID NO:1) or amino acids
79-142 (QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANEKNYQFF
IGENMNP, SEQ ID NO:2).
64. The antibody or subsequence of claim 63, wherein the fragment
of mammalian HRF/TCTP sequence comprises or consists of:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEG
EGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMT
GAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC (SEQ ID
NO:3), or a fragment 5-171 amino acid residues in length.
65.-71. (canceled)
Description
RELATED APPLICATIONS
[0001] This application claims the benefit of priority of
application Ser. No. 61/319,652 filed Mar. 31, 2010, and
application Ser. No. 61/326,079, filed Apr. 20, 2010, both of which
applications are expressly incorporated herein by reference in
their entirety.
INTRODUCTION
[0003] Histamine-releasing factor (HRF, also known as
translationally controlled tumor protein (TCTP), p21, p23, Q23, and
fortilin), is a highly conserved, multi-functional protein with
both intracellular and extracellular functions.
[0004] HRF exhibits amino acid sequence identities of over 40%
between distantly related species (Bommer et al., Int. J. Biochem.
Cell Biol. 36:379 (2004); Hinojosa-Moya et al., J. Mol. Evol.
66:472 (2008)). Fifteen of approximately 170 residues are
completely or nearly completely conserved in TCTP proteins from
yeast, pea, nematode, fruit fly, and mouse (Bommer et al., Int. J.
Biochem. Cell Biol. 36:379 (2004)). These invariant residues are
largely clustered on one side of the .beta.-stranded `core` domain.
The fold of this domain is similar to that of the Mss4/Dss4 family
of proteins, which bind to the GDP/GTP free form of Rab proteins
(members of the Ras superfamily) (Thaw et al., Nat. Struct. Biol.
8:701 (2001)). A flexible loop (TCTP1) and the C-terminal loop
(TCTP2) following the .alpha.-helices comprise the TCTP signatures.
The tubulin-binding region and the Ca2+-binding area were mapped to
the helical domain. A structural similarity was identified between
the H2-H3 helices of TCTP and the H5-H6 helices of Bax, the part of
the molecule implicated in the regulation of mitochondrial membrane
permeability during apoptosis (Susini et al., Cell Death Differ.
15:1211 (2008)).
[0005] As an intracellular protein, TCTP is involved in cell cycle
progression, proliferation, survival, and malignant transformation
of various cell types (Bommer et al., Int. J. Biochem. Cell Biol.
36:379 (2004)). The name "translationally controlled tumor protein"
was given to this protein, because TCTP mRNA levels were high but
the protein was not detected in Ehrlich acites tumor cells
(Chitpatima et al., Nucleic Acids Res. 16:2350 (1998); Yenofsky et
al., Mol. Cell. Biol. 3:1197 (1983)). TCTP is ubiquitously
expressed in all tested eukaryotic cells; its expression is active
in mitotically active tissues (Thiele et al., Eur. J. Biochem.
267:5473 (2000); Guillaume et al., Proteomics 1:880 (2001)) and
subject to both transcriptional and translational control (Bommer
et al., RNA 8:478 (2002)). It is involved in the elongation step of
protein synthesis by interacting with both eEF1A (a small GTPase)
and eEF1B.beta. (a guanine nucleotide exchange factor) (Cans et
al., Proc. Natl. Acad. Sci. USA 100:13892 (2003); Fleischer et al.,
Genes Dev. 20:1294 (2006); Langdon et al., Biochim. Biophys. Acta
3:232 (2004)). TCTP inhibits the latter activity, thus slowing down
the elongation process, avoiding `skipping`, and resulting in more
efficient elongation. G protein binding via the `core` domain seems
to be well conserved among most of TCTPs in various species.
Indeed, Drosophila TCTP acts as the guanine nucleotide-exchange
factor for Rheb, which regulates the TSC1-TSC2-mTOR pathway (Hsu et
al., Nature 445:785 (2007)). Conventional HRF/TCTP KO mice are
embryonic lethal (Chen et al., Mol. Biol. Cell 18:2525 (2007)).
These Drosophila and mouse studies strongly implicate this protein
in the regulation of growth and proliferation as well as in the
control of organ size.
[0006] Another conserved property of TCTP is its interaction with
microtubules and mitochondria (Rinnerthaler et al., Biochim.
Biophys. Acta 1757:631 (2006)). TCTP interacts with Mcl-1 (Zhang et
al., J. Biol. Chem. 277:37430 (2002); Liu et al., Mol. Cell. Biol.
25:3117-26 (2005)) and Bc1-xL (Yang et al., Oncogene 24:4778
(2005)), anti-apoptotic members of the Bc1-2 family. TCTP
antagonizes apoptosis by inserting into the mitochondrial membrane
and inhibiting Bax dimerization (Susini et al., Cell Death Differ.
15:1211 (2008)). RNA interference-mediated knockdown of TCTP
increases the frequency of tumor reversion apparently consistent
with its anti-apoptotic action of the protein (Tuyunder et al.,
Proc. Natl. Acad. Sci. USA 101:15364 (2004)). By contrast, yeast
TCTP displays proapoptotic activity, apparently via an interaction
with the outer mitochondrial membrane (Rinnerthaler et al.,
Biochim. Biophys. Acta 1757:631 (2006)).
[0007] HRF can be found in exosomes, suggesting that HRF is
secreted through a nonclassical exosome pathway (Amzallag et al.,
J. Biol. Chem. 279:46104 (2004)). HRF is also a secreted protein
and is found in nasal lavages, skin blister fluids, and
bronchoalveolar lavage (BAL) fluids during the late phase of
allergic reactions (Warner et al., J. Immunol. 136:2583 (1986);
MacDonald et al., J. Immunol. 139:506 (1987); MacDonald (1993) in
Allergy: Principles and Practice, pp 1-11, "Histamine Releasing
Factors and IgE Heterogeneity," Mosby-Year Book Incorporated, St.
Louis). HRF secretion is insensitive to brefeldin A or monensin,
but can be enhanced by TSAP6, a p53-inducible 5-6 transmembrane
protein.
[0008] Since human recombinant HRF can stimulate histamine release
and cytokine (IL-4 and IL-13) production from IgE-sensitized
basophils (MacDonald et al., Science 269:688 (1995); Schroeder et
al., J. Exp. Med. 183:1265 (1996); Schroeder et al., J. Immunol.
159:447 (1997)), it is an IgE-dependent cytokine. MacDonald et al.
revealed that cell-bound IgE is required for HRF-induced basophil
activation and identified functional heterogeneity among human IgE
molecules: IgE from HRF-responding (HRF-Responder) basophils
derived from .about.50% of atopic patients was termed IgE+, and IgE
from nonresponders (HRF-Nonresponders) was termed IgE- (MacDonald
et al., Int. Arch. Allergy Immunol. 113:187 (1997)).
[0009] HRF was also isolated as a B cell growth factor (Kang et
al., J. Immunol. 166:6545 (2001)), and can stimulate IL-8 secretion
from GM-CSF-primed eosinophils (Bheekha-Escura et al., Blood
96:2191 (2000)). HRF was reported to stimulate bronchial epithelial
cells to produce IL-8 and GM-CSF (Yoneda et al., Am. J. Physiol.
Lung Cell Mol. Physiol. 286:L174 (2004)). Despite intensive
efforts, the exact molecular basis of the IgE+/IgE- dichotomy has
remained an enigma. For example, heterogeneity in the carbohydrate
portion of IgE molecules fails to distinguish between IgE+ and
IgE-(Kleine-Tebbe et al., J. Allergy Clin. Immunol. 98:181 (1996)).
On the other hand, the releasability of human basophils in response
to anti-IgE was correlated positively with Syk levels (Kepley et
al., J. Allergy Clin. Immunol. 104:279 (1999); Lavens-Phillips et
al., Am. J. Respir. Cell Mol. Biol. 23:566 (2000); MacGlashan et
al., J. Allergy Clin. Immunol. 119:626 (2007)) and negatively with
SHIP (SH2 domain-containing phosphatidylinositol 5' phosphatase)
levels (MacGlashan et al., J. Allergy Clin. Immunol. 119:626
(2007)). Interestingly, HRF responses in human basophils were shown
to negatively correlate with SHIP, but not Syk, levels (Vonakis et
al., J. Allergy Clin. Immunol. 108:822 (2001)), explaining some
HRF-Responder subjects.
[0010] HRF-triggered signaling in human basophils was found to be
identical or similar to those induced by anti-IgE stimulation of
human basophils (Vonakis et al., Blood 111:1789 (2008)) and by
antigen stimulation of IgE-sensitized mast cells: 1) stimulation
with HRF was not sensitive to pertussis toxin, similar to
anti-IgE/IgE-induced basophil activation. 2) Tyrosine
phosphorylation of Syk was induced, and a Syk inhibitor blocked
HRF-induced histamine release. A recent study also showed loss of
Syk protein in human basophils stimulated with HRF similar to that
induced by anti-IgE (MacGlashan et al., J. Immunol. 180:4208
(2008)). 3) Increased intracellular Ca2+ and Ca2+/MEK-dependent
leukotriene C4 release (MacGlashan and Hubbard, J. Immunol.
151:6358 (1999)) were induced by HRF in HRF-Responder, but not
HRF-Nonresponder, basophils. 4) HRF-induced histamine release was
inhibited by the phosphatidylinositol 3-kinase (PI3K) inhibitor
Ly294002 (Vonakis et al., J. Allergy Clin. Immunol. 108:822
(2001)), and phosphorylation of Akt, a PI3K-dependent event, was
induced by HRF in HRF-Responder, but not HRF-Nonresponder,
basophils. 5) MEK and ERK phosphorylation was induced by HRF in
HRF-Responders, but not HRF-Nonresponder, basophils.
[0011] Consistent with the similarities in signaling between
HRF-receptor and Fc.epsilon.RI, glucocorticoids were shown to
inhibit IL-4 production from HRF-stimulated human basophils at the
transcriptional level (Schroeder et al., J. Immunol. 158:5448
(1997)). However, differences were also reported in that no
phosphorylation of Fc.epsilon.RI.gamma. (=FcRy) was found in
HRF-stimulated basophils (Vonakis et al., Blood 111:1789 (2008)).
However, this failure may be due to low levels of phosphorylation
and limited cell numbers used. A pharmacological study showed that
rottlerin, which inhibits protein kinase C(PKC)-.delta. and
PKC-.theta. (Coudronniere et al., Proc. Natl. Acad. Sci. USA
97:3394 (2000)), enhances HRF-mediated histamine release without
affecting basophil activation by either anti-IgE or antigen,
although staurosporine, Bis II, Go 6976, or pertussis toxin cannot
differentiate histamine release induced by anti-IgE or antigen from
that induced by HRF (Bheekha-Escura et al., J. Allergy Clin.
Immunol. 103:937 (1999)).
[0012] Most studies on HRF have been performed with human
basophils. However, the role of HRF, if any, in allergic and other
immune diseases has been elusive for decades. For example, a
clinical study failed to find a correlation between bronchial
late-phase responses to Dermatophagoides pteronyssinus (a house
dust mite) and IgE reactivity to HRF produced from PBMCs (Budde et
al., All Allergy Asthma Immunol. 89:606 (2002)).
[0013] The lack of understanding of HRF in allergic and other
immune diseases may derive from factors such as that the HRF
receptor has not been identified, functional validation with animal
models of allergic disease has not verified HRF in allergic and
other immune diseases, or has an analysis of the HRF gene been
performed on a large population of allergic patients. In addition
to these, the study of HRF has another formidable obstacle: how can
the extracellular (=cytokine) and intracellular functions of HRF be
distinguished? Simple overexpression (by transgenic approach),
knockout, or knockdown of the HRF gene cannot resolve this problem.
Even conditional knockout techniques will not provide an answer, as
the intracellular function of HRF/TCTP in the targeted cells might
be affected at the same time. Little is known about how HRF, which
does not have a signal sequence, is secreted.
[0014] As disclosed herein, one way in which to determine whether
HRF has a role, if any, in allergic and other immune diseases, is
by potentiation of HRF function or activity, and the other could be
inhibition of HRF function or activity. As disclosed herein,
identification of an HRF receptor (HRF-R) and a representative
inhibitor of HRF/HRF-R (R=receptor) interaction has been
identified, and is a representative modulator of HRF's cytokine
function, and has revealed the role of HRF in allergic and other
immune diseases.
[0015] The prevalence of asthma and other allergic diseases has
increased dramatically for the last few decades and has reached
epidemic proportions in the western populations (Eder, W. et al., N
Engl. J. Med. 355:2226 (2006)). Allergic patients suffer from
organ-specific manifestations, while the same pathogenic mechanism
appears to underlie these diseases. For example, asthma is
characterised by lung inflammation, airway hyper-responsiveness
(AHR), airway remodeling, and reversible bronchoconstriction; food
allergy is manifested by various gastrointestinal, pulmonary and
cutaneous signs and symptoms. After binding of allergen-specific
IgE to mast cells, susceptible individuals respond to allergens by
releasing mast cell-derived mediators. Subsequent allergen exposure
produces a cascade of events orchestrated by immune effector cells
such as T-helper type 2 (Th2) cells, eosinophils, and mast cells
(Gould, H. J. et al. Nat. Rev. Immunol. 8:205 (2008)). Indeed, the
pathogenic role of Th2 cytokines such as IL-4, IL-5, IL-9, and
IL-13 in various aspects of asthma has been shown in mouse and
human studies (Boyce, J. A. et al. J. Exp. Med. 201:1869 (2005)).
IgE, produced by B cells stimulated by IL-4 or IL-13, (Geha, R. S.,
et al. Nat. Rev. Immunol. 3:721 (2003)) also plays a significant
role in asthma, as anti-IgE therapy is efficacious in treating
asthmatics (Barnes, P. J. Int. Arch. Allergy Immunol. 123:196
(2000)). Further, the high-affinity receptor (Fc.epsilon.RI) of IgE
and mast cells play a significant role in some asthma models
(Kobayashi, T. et al. J. Immunol. 164:3855 (2000); Williams, C. M.
et al. J. Exp. Med. 192:455 (2000); Taube, C. et al. J. Immunol.
172:6398 (2004)).
[0016] Food allergies typically affect .about.6% of young children
and 3-4% of adults (Sampson et al., J. Allergy Clin. Immunol.
113:805 (2004); Sicherer et al., J. Allergy Clin. Immunol. 114:159
(2004)). In the US, food allergy alone accounts for about 30,000
anaphylactic reactions, 2,000 hospital admissions, and 200 deaths
each year (Yocum et al., J. Allergy Clin. Immunol. 104:452 (1999);
Burks, Lancet 371:1538 (2008)). Peanuts, tree nuts, fish, and
shellfish are common allergens in both children and adults, while
children also often react to eggs, wheat, and soy.
[0017] Food-induced allergic reactions result from immunologic
pathways that include activation of effector cells through food
specific IgE antibodies, cell-mediated (non-IgE-mediated) reactions
resulting in subacute or chronic inflammation, or the combination
of these pathways. The significance of IgE-mediated arm of
reactions in human was demonstrated by anti-IgE therapy in patients
with peanut allergy, which significantly and substantially
increased the threshold of sensitivity to peanut on oral food
challenge (Leung et al., N. Engl. J. Med. 348:986 (2003)).
Furthermore, histamine has been reported to increase in food
allergy patients after allergen challenge (Sampson and Jolie, N.
Engl. J. Med. 311:372 (1984)), suggesting the involvement of mast
cell or basophil activation downstream of IgE-mediated pathways. On
the other hand, celiac disease, which is a representative of the
cell-mediated arm of food hypersensitivity, is mediated by
gluten-reactive T cells, and the symptoms are confined to gut,
often mild and chronic (Sollid and Lundin, Mucosal Immunol. 2:3
(2009)).
[0018] Systemic anaphylaxis mouse models revealed two major
pathways (Finkelman, J. Allergy Clin. Immunol. 120:506 (2007)),
both of which depend on immunoglobulins (Igs). The signals of the
classic pathway start from IgE and its high-affinity receptor,
Fc.epsilon.RI, on mast cells, and the subsequent release of
histamine and platelet activating factor (PAF) causes the
anaphylactic symptoms. Indeed, blockade of histamine can prevent
the hypothermia triggered by this pathway of anaphylaxis
(Makabe-Kobayashi et al., J. Allergy Clin. Immunol. 110:298
(2002)). The alternative pathway depends upon IgG/Fc.gamma.RIII
signaling on macrophages or basophils, which leads to PAF release
(Strait et al., J. Allergy Clin. Immunol. 109:658 (2002)). Small
doses of antigen favor the classic pathway, while the large doses
are required for the alternative pathway (Strait et al., J. Clin.
Invest. 116:833 (2006)). Since only a small proportion of orally
administered antigen participates in the systemic circulation in an
immunologically intact form (Warshaw et al., Lab Invest. 25:675
(1971)), the contribution of the alternative pathway towards food
allergy should be interpreted with caution (Berin and Mayer,
Mucosal Immunol. 2:24 (2009)). In a cholera toxin-induced model of
peanut allergy (Li et al., J. Allergy Clin. Immunol. 106:150
(2000)) in C57BL/6 background mice (Sun et al., J. Immunol.
179:6696 (2007)), anaphylaxis was completely abolished in
B-cell-deficient or mast cell-deficient mice, whereas
Fc.epsilon.RIa-deficient mice showed significantly milder
anaphylactic responses (Sun et al., J. Immunol. 179:6696
(2007)).
[0019] There are also two major food-induced diarrhea models, in at
least one of which the Ig/mast cell axis was shown to play a role.
In that model, mice were sensitized with OVA plus alum injected
intraperitoneally, and fed with OVA (Brandt et al., J. Clin.
Invest. 112:1666 (2003)). This model has increased mast cells in
the small intestine, especially in jejunum. Mast cell depletion by
anti-c-Kit antibody abrogated the diarrhea, while Fc.epsilon.RIa
knockout mice showed delayed and decreased incidence of diarrhea
development (Brandt et al., J. Clin. Invest. 112:1666 (2003)).
Adoptive transfer of mesenteric lymph node CD4.sup.+ T cells could
transfer the sensitization to naive mice. However, several
challenges of OVA were needed for mast cell accumulation and
diarrhea development after transfer (Knight et al., Am. J. Physiol.
Gastrointest. Liver Physiol. 293:G1234 (2007)). IL-9 was shown to
be important for mast cell accumulation and diarrhea development
(Forbes et al., J. Exp. Med. 205:897 (2008)). The other model uses
OVA plus CFA injected subcutaneously, and fed with OVA (Kweon et
al., J. Clin. Invest. 106:199 (2000)). In this model, the increase
of mast cells was observed in colon, not in the small intestine.
IL-4 and Stat6 were shown to be indispensable for diarrhea
occurrence (Kweon et al., J. Clin. Invest. 106:199 (2000)). When
signaling of sphingosine 1-phosphate was blocked by FTY 720, the
recruitment of mast cells and Th2 cells were inhibited, and
diarrhea was abolished without affecting increased IgE level
(Kurashima et al., J. Immunol. 179:1577 (2007)).
[0020] Mononuclear cells from food allergy patients have been
reported to secrete HRF, and the spontaneous ex vivo histamine
release from basophils were reportedly increased in those patients
(May, J. Allergy Clin. Immunol. 58:432 (1976); May and Remigio,
Clin. Allergy 12:299 (1982); Sampson et al., N. Engl. J. Med.
321:228 (1989)). This spontaneous histamine release decreased after
elimination of allergenic food, and patients who adhered to a
restricted diet had an apparently declined rate of spontaneous
generation of HRF in mononuclear cells (Sampson et al., N. Engl. J.
Med. 321:228 (1989)). However, these studies were based on
histamine releasing activity of serum on basophils, not on direct
measurement of HRF. In addition, the impact of spontaneous release
of histamine from basophils on food allergy pathogenesis has not
been studied. Thus, the role of HRF in food allergy remains
unclear.
[0021] New manifestations of food allergy are also increasing in
recognition and prevalence. The most common of these is
eosinophilic esophagitis (EoE) (Furuta et al., Gastroenterology
133:1342 (2007)). Symptoms of EoE include vomiting, abdominal pain,
and failure to thrive in young children which progress to
predominant complaints of dysphagia in adolescents and adults
(Furuta et al., Gastroenterology 133:1342 (2007)). Clinically, EoE
is difficult to distinguish from other forms of esophagitis,
specifically gastroesophageal reflux disease (GERD) (Furuta et al.,
Gastroenterology 133:1342 (2007)). However, in stark contrast to
GERD, EoE is successfully treated using empiric or skin prick/skin
patch-directed elimination diets and elemental formulas (Kagalwalla
et al., Clin. Gasteroenterol. Hepatol. 4:1097 (2006); Spergel et
al., Ann. Allergy Asthma Immunol. 95:336 (2005); Spergel et al., J.
Allergy Clin. Immunol. 119:509 (2007)). Indeed, elemental formula
is one of the most effective therapeutic regimens in EoE with
patients demonstrating >96% response rates (Markowitz et al.,
Am. J. Gastroenterol. 98:777 (2003)). The most significant
complication of EoE is esophageal stricture formation due to tissue
remodeling (Aceves et al., J. Allergy Clin. Immunol. 119:206
(2007); Fruman et al., Immunity 13:1 (2000)). However, food
impactions can occur even in the absence of strictures, likely due
to the significant esophageal dysmotility described in both adult
and pediatric EoE patients (Furuta et al., Gastroenterology
133:1342 (2007); Korsapati et al., Gut 58:1056 (2009); Nurko et
al., Am. J. Gastroenterol. 104:3050 (2009); Remedios et al.,
Gastrointest. Endosc. 63:3 (2006)).
[0022] Although the majority of patients with EoE (approximately
70%) have food sensitization, the role of IgE mediated food allergy
in EoE remains relatively unclear (Furuta et al., Gastroenterology
133:1342 (2007)). Recent reports suggest that there is increased
local IgE production in addition to the systemic sensitizations
that occur in EoE (Vicario et al., Gut 59:12 (2010)). In addition,
delayed type hypersensitivity and a dependence on T cells also play
a role in EoE (Mishra et al., J. Leukoc. Biol. 81:916 (2007)).
Although defined by the presence of a diffuse eosinophilia of the
esophagus (>15 eosinophils per high power field despite adequate
acid blockade), EoE is accompanied by a significant esophageal
mastocytosis (Kirsh et al., J. Pediatric Gastroenterol. Nutrition
44:20 (2007)). Mast cells tend to be degranulated in EoE patients
(Kirsh et al., J. Pediatric Gastroenterol. Nutrition 44:20 (2007)).
As such, the previously unstudied role of mast cell activating
factors such as HRF in EoE patients is important and may lend new
therapeutic options in these patients.
[0023] Thus, there is a need for compounds and methods of treating
immune diseases and allergic reactions, such as food allergies,
airway inflammation, and hypersensitivity. This invention addresses
this need and provides related benefits.
SUMMARY
[0024] The invention provides methods of treating a food allergy.
In one embodiment, a method includes contacting histamine releasing
factor (HRF)/translationally controlled tumor protein (TCTP) with a
compound that inhibits or reduces binding of HRF/TCTP to an
immunoglobulin thereby treating a food allergy.
[0025] The invention also provides methods of treating an allergic
reaction, hypersensitivity, an inflammatory response or
inflammation. In one embodiment, a method includes contacting
histamine releasing factor (HRF)/translationally controlled tumor
protein (TCTP) with a compound that inhibits or reduces binding of
HRF/TCTP to an immunoglobulin thereby treating the allergic
reaction, hypersensitivity, inflammatory response or
inflammation.
[0026] The invention further provides methods of reducing or
decreasing the probability, severity, frequency, duration or
preventing a subject from having an acute or chronic food allergy,
allergic reaction, hypersensitivity, an inflammatory response or
inflammation. In one embodiment, a method includes administering to
a subject a compound that inhibits or reduces binding of HRF/TCTP
to an immunoglobulin thereby decreasing the probability, severity,
frequency, duration or preventing the subject from having an acute
or chronic food allergy, allergic reaction, hypersensitivity,
inflammatory response or inflammation.
[0027] Compounds include peptides and polypeptides. Non-limiting
exemplary peptides and polypeptides include antibody and antibody
subsequences (polyclonal and monoclonal). Antibodies include
mammalian antibodies, such as human and humanized antibodies and
subsequences. Additional non-limiting exemplary peptides and
polypeptides include an HRF/TCTP (e.g., mammalian) polypeptide, or
a subsequence or fragment of an HRF/TCTP (e.g., mammalian)
polypeptide, that binds to an immunoglobulin. In particular
aspects, a subsequence or fragment of HRF/TCTP polypeptide that
binds to an immunoglobulin includes or consists of amino acids 1-19
or amino acids 79-142 of a HRH/TCTP sequence (e.g., mammalian), or
a subsequence thereof. In additional particular aspects, a
subsequence or fragment of HRF/TCTP polypeptide that binds to an
immunoglobulin includes or consists of MIIYRDLISHDEMFSDIYK (SEQ ID
NO:1), or QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQ
FFIGENMNP (SEQ ID NO:2) sequence, or a subsequence thereof.
Immunoglobulins to which HRH/TCTP sequence bind include, IgG, IgE,
IgA, IgM, or IgD
[0028] Methods of the invention are useful for treatment of a food
allergy, allergic reaction, hypersensitivity, inflammatory response
or inflammation, chronic or acute. Methods of the invention include
those sufficient to protect against the food allergy, allergic
reaction, hypersensitivity, inflammatory response or inflammation,
decrease, reduce, inhibit, suppress, limit or control
susceptibility to the food allergy, allergic reaction or
hypersensitivity, or decrease, reduce, inhibit, suppress, limit or
control the food allergy, allergic reaction, hypersensitivity,
inflammatory response or inflammation. Methods of the invention
also include those sufficient to decrease, reduce, inhibit,
suppress, limit, control or improve the probability, severity,
frequency, or duration of one or more adverse symptoms, disorders,
illnesses, pathologies, diseases, or complications caused by or
associated with the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation. Methods of
the invention further include those sufficient to reduce or inhibit
progression, severity, frequency, duration or probability of an
adverse symptom of the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation.
[0029] Methods of the invention are also useful for treatment of
more particular allergic reactions, such as extrinsic or intrinsic
bronchial asthma; Allergic rhinitis; Onchocercal dermatitis; Atopic
dermatitis; eczema; rash; allergic urticaria (e.g. hives); allergic
conjunctivitis; Drug reactions; Nodules, eosinophilia, rheumatism,
dermatitis, and swelling (NERDS); Eosophageal and a
gastrointestinal allergy. Methods of the invention are also useful
for treatment of more types of hypersensitivity, inflammatory
response or inflammation, such as a respiratory disease or disorder
(e.g., that affects the upper or lower respiratory tract).
Non-limiting exemplary respiratory diseases and disorders include
asthma, allergic asthma, bronchiolitis, pleuritis, Airway
Obstruction, Apnea, Asbestosis, Atelectasis, Berylliosis,
Bronchiectasis, Bronchiolitis, Bronchiolitis Obliterans Organizing
Pneumonia, Bronchitis, Bronchopulmonary Dysplasia, Empyema, Pleural
Empyema, Pleural Epiglottitis, Hemoptysis, Hypertension, Kartagener
Syndrome, Meconium Aspiration, Pleural Effusion, Pleurisy,
Pneumonia, Pneumothorax, Respiratory Distress Syndrome, Respiratory
Hypersensitivity, Rhinoscleroma, Scimitar Syndrome, Severe Acute
Respiratory Syndrome, Silicosis, Tracheal Stenosis, eosinophilic
pleural effusions, Histiocytosis; chronic eosinophilic pneumonia;
hypersensitivity pneumonitis; Allergic bronchopulmonary
aspergillosis; Sarcoidosis; Idiopathic pulmonary fibrosis;
pulmonary edema; pulmonary embolism; pulmonary emphysema; Pulmonary
Hyperventilation; Pulmonary Alveolar Proteinosis; Chronic
Obstructive Pulmonary Disease (COPD); Interstitial Lung Disease;
allergic rhinoconjunctivitis; allergic conjunctivitis and Topical
eosinophilia.
[0030] Methods of the invention are further useful for treatment of
more particular allergic reactions, hypersensitivity, inflammatory
response or inflammation, such as a skin or eye allergic reaction,
hypersensitivity, inflammatory response or inflammation.
[0031] The invention moreover provides methods of increasing,
enhancing or stimulating airway-dilation. In one embodiment, a
method includes administering to a subject in need of increasing
airway-dilation an amount of a compound that inhibits or reduces
binding of HRF/TCTP to an immunoglobulin sufficient to increase,
enhance or stimulate airway-dilation in the subject.
[0032] The invention additionally provides methods of reducing or
inhibiting airway-constriction. In one embodiment, a method
includes administering to a subject in need thereof an amount of a
compound that inhibits or reduces binding of HRF/TCTP to an
immunoglobulin sufficient to reduce or inhibit airway-constriction
in the subject.
[0033] Compounds may be administered at any time relative to the
condition to be treated. In particular embodiments, a compound is
administered prior to, substantially contemporaneously with or
following one or more adverse symptoms, disorders, illnesses,
pathologies, diseases, or complications caused by or associated
with the disease or disorder, for example, food allergy, allergic
reaction or hypersensitivity. In more particular embodiments, a
compound is administered to a subject substantially
contemporaneously with, or within about 1-60 minutes, hours, or
days of the onset of an adverse symptom associated with the disease
or disorder, for example, a food allergy, allergic reaction or
hypersensitivity.
[0034] Compounds may be administered by any route, locally,
regionally or systemically. In particular embodiments, a compound
is administered via ingestion, via inhalation, or topically. A
compound can be administered one, two, three, four or more times
daily, weekly, monthly, bi-monthly, or annually, to a subject.
[0035] The amount of compound administered can be in an amount
likely sufficient or effective to provide a response to a subject.
In particular embodiments, a compound is from about 0.00001 mg/kg
to about 10,000 mg/kg, from about 0.0001 mg/kg to about 1000 mg/kg,
from about 0.001 mg/kg to about 100 mg/kg, from about 0.01 mg/kg to
about 10 mg/kg, or from about 0.1 mg/kg, about 1 mg/kg body
weight.
[0036] The invention still also provides methods of diagnosing a
subject having or at risk of a food allergy. In one embodiment, a
method includes measuring histamine releasing factor
(HRF)/translationally controlled tumor protein (TCTP) in a sample
from a subject, wherein an amount of HRF/TCTP in the sample greater
than normal diagnoses the subject as having or at risk of a food
allergy. Non-limiting examples of measuring include, for example,
determining the amount of HRF/TCTP protein or nucleic acid encoding
HRF/TCTP in the sample; contacting the sample with an agent or tag
that binds to HRF/TCTP protein or nucleic acid encoding HRF/TCTP
and ascertaining the amount of HRF/TCTP protein or nucleic acid
encoding HRF/TCTP, or the amount of agent or tag bound to the
HRF/TCTP protein or nucleic acid encoding HRF/TCTP.
[0037] The invention yet further provides methods of identifying an
agent that reduces or inhibits a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation. In one
embodiment, a method includes contacting histamine releasing factor
(HRF)/translationally controlled tumor protein (TCTP) with a test
compound in the presence of an immunoglobulin that binds to
HRF/TCTP; and determining if the compound inhibits or reduces
binding of HRF/TCTP to the immunoglobulin, wherein a reduction or
inhibition of binding identifies the test compound as an agent that
reduces or inhibits a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation.
[0038] Subjects include any animal in need of treatment, such as a
subject in need of treatment for a food allergy, allergic reaction,
hypersensitivity, an inflammatory response or inflammation. In
particular embodiments, a subject is a mammal (e.g., a human).
[0039] The invention still moreover provides subsequences of
mammalian HRF/TCTP sequence less than full length HRF, wherein the
subsequence binds to an immunoglobulin. In one embodiment, a
subsequence includes or consists of amino acids 1-19 (e.g.,
MIIYRDLISHDEMFSDIYK (SEQ ID NO:1)) or amino acids 79-142 (e.g.,
QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQ FFIGENMNP
(SEQ ID NO:2)) of a mammalian HRF. In another embodiment, a
subsequence includes or consists of a subsequence of a mammalian
HRF/TCTP set forth
as:MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAE
GPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPER
VKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFK DGLEMEKC
(SEQ ID NO:3), wherein the subsequence is between about 5-171 amino
acid residues in length, and binds to an immunoglobulin. In
particular aspects, a subsequence has a length from about 5-10,
10-20, 20-50, 100-150, or 150-171 amino acid residues.
[0040] The invention still further provides isolated and purified
antibody or antibody subsequence that binds to mammalian HRF/TCTP
sequence, which sequence includes or consists of amino acids 1-19
(MIIYRDLISHDEMFSDIYK (SEQ ID NO:1)) or amino acids 79-142
(QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNY QFFIGENMNP
(SEQ ID NO:2)), or binds to a fragment of mammalian HRF/TCTP
sequence set forth as
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGP
EGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVK
PFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDG LEMEKC (SEQ
ID NO:3), wherein the fragment is 5-171 amino acid residues in
length. In particular aspects, a subsequence has a length from
about 5-10, 10-20, 20-50, 100-150, or 150-171 amino acid
residues.
DESCRIPTION OF THE DRAWINGS
[0041] FIGS. 1A-1D show A) HRF-bound mouse IgE by ELISA; B)
detection of IgE and GST proteins by immunoblotting with anti-mouse
IgE antibody and anti-GST mAb, respectively; C) HRF binding
detected by flow cytometry when WT, but not Fc.epsilon.RIa.sup.-/-,
BMMCs were incubated with an HRF-reactive (C38-2), but not a
nonreactive (C48-2, left panel), IgE mAb. Insets show IgE binding:
BMMCs preincubated with or without (gray) the indicated IgE were
incubated with FITC-labeled anti-mouse IgE; and D) the numbers of
mAbs classified into IgG subtypes and K.sub.D values of some IgG
molecules to HRF are shown as detected by ELISA.
[0042] FIG. 2 shows HRF-bound human IgEs detected by incubation
with biotin-conjugated anti-human IgE antibody, followed by
incubation with horseradish peroxidase-conjugated streptavidin.
Absorbance at 450 nm was measured after development of the color.
Data indicate mean.+-.SEM. n.d.=not detected.
[0043] FIGS. 3A-3C show A) the scheme of full-length (FL) and
truncated forms of GST-mHRF used for IgE binding; B) HRF-bound IgE
detected using anti-mouse IgE by ELISA; and C) HRF-bound-IgE in the
absence (-) or presence (+) of competitors as detected by
incubation with biotinylated anti-mouse IgE mAb, followed by
streptavidin-HRP.
[0044] FIG. 4 shows HRF-bound IgGs detected by incubation with
horseradish peroxidase-conjugated antimouse IgG antibody.
Absorbance at 450 nm was measured after development of the color.
Data indicate mean.+-.SEM.
[0045] FIGS. 5A-5B show A) cells cultured in the absence (-) or
presence of the indicated concentrations of GST or GST-N19 for 2
days, and live cells detected by the exclusion of Trypan blue; and
B) live BMMC or CHO-K1 cells after IL-3 withdrawal or
H.sub.2O.sub.2 induced apoptosis, respectively. Data indicate
mean.+-.SEM.
[0046] FIGS. 6A-6E show A) BMMC engrafted W.sup.sh/W.sup.sh mice
which were confirmed by Toluidine Blue staining have levels of mast
cells similar to those of WT mice. HRF or negative and positive
controls, saline (S) and TNP.sub.26-BSA (Ag), respectively, were
injected in IgE (C38-2 or C48-2) sensitized ears and vascular
permeability was measured after 30 minutes; B) acute reactions in
Fc.epsilon.RIa.sup.-/- mice were not induced; C) Late Phase
Reactions (LPR) were analyzed by measurement of ear thickness at 8
hours after injection of mHRF-His.sub.6 (H) in IgE (C38-2 or
C48-2)-sensitized ears. Negative and positive controls, saline (S)
and TNP.sub.26-BSA (Ag), respectively, were injected in sensitized
ears; D) .beta.-hexosaminidase release from peritoneal mast cells
sensitized with the HRF-reactive, but not the HRF-nonreactive, IgE
were activated upon stimulation with HRF; and E) WT mice sensitized
with the indicated IgE. Ear thickness was measured over a 10 hour
period. *, **, ***: p<0.05, p<0.01, p<0.001 by Student's
t-test.
[0047] FIGS. 7A-7B show A) ear thickness of mice sensitized with
IgE in the presence of saline, TNP.sub.26-BSA, or HRF B) H&E
staining of sensitized mouse ears. Bar=100 .mu.m. Data indicate
mean.+-.SEM. *, **: p<0.05, p<0.01 by Student's t-test.
[0048] FIGS. 8A-8F show A) immunoblotting on lung homogenates and
plasma samples of HRF expression levels. For loading control,
ERK1/2 expression was analyzed; B) immunofluorescence microscopy on
membrane-permeabilized or non-permeabilized lung tissues. HRF was
stained red. The plasma membrane was stained with wheat germ
agglutinin (green) and the nuclei with DAPI (blue). Bar=200 .mu.m;
C) total and specific immune cell numbers in BAL fluids were
enumerated. Eos, eosinophils; Neu, neutrophils; Lym, lymphocytes;
Mono, monocytes; D) paraffin-embedded lung tissues stained by
H&E and periodic acid-Schiff (PAS). Bar=200 .mu.m; E) IL-5 and
IL-13 in lung homogenates (PBS [P], OVA [0], OVA+GST [0], and
OVA+N19 [19]) measured by ELISA; and F) that GST-N19 treatment
inhibited airway hyper-responsiveness (AHR). Data indicate
mean.+-.sem. *, **, ***: p<0.05, p<0.01, p<0.001 by
Student's t-test.
[0049] FIGS. 9A-9B show HRF and HRF-reactive IgG measured by ELISA.
OVA-immunized mice were intranasally challenged with OVA or PBS.
Some mice were pretreated with GST- or GST-N19 before each OVA
challenge. Twenty-four hours after the last challenge, the mice
were sacrificed and BAL fluids and plasma were collected. HRF in
plasma (A) and HRF-reactive IgG (B) in plasma and BAL fluids were
measured by ELISA. Data represent mean.+-.SEM. *, p<0.05 by
Student's t-test.
[0050] FIG. 10 shows Ig levels in plasma from OVA-immunized mice
challenged with OVA or PBS pretreated with GST- or GST-N19, before
each OVA challenge. OVA-specific IgE, IgG1, and IgG2a were measured
by ELISA. Data represent mean.+-.SEM. *, p<0.05 by Student's
t-test.
[0051] FIGS. 11A-11E show A) total and specific immune cell numbers
in BAL fluids. Eos, eosinophils; Neu, neutrophils; Lym,
lymphocytes; Mono, monocytes; M.phi., macrophages; B)
Paraffin-embedded lung tissues stained by H&E and periodic
acid-Schiff (PAS). Bar=200 .mu.m; C) IL-13 suppression by GST-N19;
and that GST-N19 does not inhibit T cell-dependent/mast
cell-independent airway inflammation. D) Paraffin-embedded lung
tissues stained by H&E and periodic acid-Schiff (PAS). Bar=100
.mu.m. E) Total and specific immune cell numbers in BAL fluids were
enumerated. Data indicate mean.+-.sem. *, **, ***: p<0.05,
p<0.01, p<0.001 by Student's t-test.
[0052] FIGS. 12A-12B show GST-N19 inhibits Aspergillus fumigatus
allergen-induced airway inflammation. BALB/c mice were lightly
anesthetized by isoflurane inhalation, and 50 .mu.l of Aspergillus
allergen or PBS was applied to the bares. Mice were immunized three
times per week for 3 weeks, as described previously (Mathias et
al., J. Immunol. 182:2416 (2009)). Some mice were intranasally
pretreated with GST or GST-N19 (200 mg/50 ml) from the second week
30 min before each immunization. Twenty-four hours after the last
challenge, mice were sacrificed and BAL fluids and lung tissues
were collected. A) Total and specific immune cell numbers in BAL
fluids were enumerated. Eos, eosinophils; Neu, neutrophils; Lym,
lymphocytes; Mono, monocytes. B) Paraffin-embedded lung tissues
were stained by H&E and periodic acid-Schiff (PAS). Bar=200
.mu.m. Data indicate mean.+-.sem. *, **, ***: p<0.05, p<0.01,
p<0.001 by Student's t-test.
[0053] FIG. 13 shows weak lung inflammation is induced by HRF alone
in naive mice. Differential cell counting was performed on cytospin
preparations stained with May-Giemsa. P values by Student's t-test
are shown.
[0054] FIG. 14 shows GST-N19 prevents the development of diarrhea.
The development of diarrhea was monitored for ninety minutes after
OVA challenge. Log-rank test: p=0.028 (GST vs. GST-N19), p=0.036
(Control vs. GST-N19). Five or six mice per group.
[0055] FIGS. 15A-15D show GST-N19 inhibits mastocytosis in the
small intestine in the OVA-induced food allergy model. A)
representative results of the jejunal sections. Sections indicated
by rectangles are enlarged in lower rows; B) mucosal; C) serosal
mast cells were enumerated in each high-power field (HPF); and D)
correlation was found between diarrhea occurrence and numbers of
mucosal mast cells. Spearman's r=0.8262 (r.sup.2=0.68),
p<0.0001.
[0056] FIGS. 16A-16E show plasma levels of A) IgE; B) IgG1; C)
IgG2a measured using commercial ELISA kits; D) HRF-reactive IgG
measured in ELISA using mHRF-His.sub.6 as a capturing agent. After
incubation with plasma (diluted 200 folds), bound IgG was detected
with HRP-conjugated anti-mouse IgG. *, **, ***: p<0.05, 0.01,
p<0.001 by Student's t-test; and E) Immunoblot analysis of
plasma HRF levels. Two samples per group were analyzed.
[0057] FIG. 17 shows ELISA of sera from food allergy patients
stratified by IgE levels (+, .gtoreq.0.35 kU/L; -, <0.35 kU/L)
and anaphylaxis to measure HRF-reactive IgG. Each cohort consists
of 4 or 6 patients.
[0058] FIG. 18 shows HRF as both monomeric and dimeric forms.
Positions of monomer and dimer are indicated. Monomer* may be a
form of monomer with intramolecular disulfide bonding.
[0059] FIG. 19 shows HRF-reactive IgE binding to both monomeric and
dimeric forms of HRF. The absorbance at 450 nm was measured after
development of the color. Data indicate mean.+-.SEM.
[0060] FIG. 20 shows the scheme of HRF-mediated IgE/Fc.epsilon.RI
crosslinking. The top view (Top) of IgE at the level of Fab and the
side view (Bottom) of IgE and IgE-bound Fc.epsilon.RI .alpha. chain
are shown on the left. Formed Fc.epsilon.RI .alpha. chain-nucleated
complexes with HRF are shown on the right. The cytoplasmic portion
of Fc.epsilon.RI .alpha. as well as .beta. and .gamma. chains of
Fc.epsilon.RI are omitted for clarity.
[0061] FIG. 21 shows an alignment of representative mammalian HRF
sequences (SEQ ID NOs:4-10).
DETAILED DESCRIPTION
[0062] The invention is based, at least in part, on histamine
releasing factor (HRF)/translationally controlled tumor protein
(TCTP), and the identification of the HRF receptor (HRF-R) and
inhibitors of HRF/HRF-R interactions. The invention is also based,
at least in part, on identifying the role of HRF in food allergies,
airway inflammation and skin or eye hypersensitivity.
[0063] In accordance with the invention, polypeptide (e.g., HRF,
antibodies) sequences, such as substantially isolated, purified,
and recombinant polypeptides, e.g., that bind to an immunoglobulin
(Ig), are provided. In one embodiment, a polypeptide sequence is
characterized as including or consisting of a subsequence of HRF
(e.g., mammalian HRF) which binds to an immunoglobulin. In another
embodiment, a polypeptide sequence is characterized as including or
consisting of HRF amino acids 1-19 (e.g., MIIYRDLISHDEMFSDIYK (SEQ
ID NO:1)) or HRF amino acids 79-142 (e.g.,
QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQ FFIGENMNP
(SEQ ID NO:2)), or a subsequence of HRF amino acids 1-19 or HRF
amino acids 79-142, and which subsequence binds to an
immunoglobulin. In another embodiment, a polypeptide sequence is
characterized as including or consisting of a subsequence of
mammalian HRF/TCTP HRF amino acids
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGP
EGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVK
PFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDG LEMEKC (SEQ
ID NO:3), where the sequence is not full length HRF, and has
between about 5-171 HRF amino acid residues in length, and which
sequence binds to an immunoglobulin. Non-limiting exemplary
sequences less than full length HRF sequence include, for example,
5-10, 10-20, 20-30, 30-40, 40-50, 50-75, 75-100, 100-150, and
150-171 amino acid residues of HRF sequence.
[0064] Exemplary mammalian HRF sequences include human and
non-human HRF sequences. Exemplary Human (NM.sub.--003295), Mouse
(NM.sub.--009429), Rat (NM.sub.--053867), Rabbit
(NM.sub.--001082129), Guinea Pig (NM.sub.--001173082), Chimpanzee
(NM.sub.--001098546), Monkey (NM.sub.--001095869), Dog
(NM.sub.--851473), Pig (NM.sub.--214373), Bovine
(NM.sub.--001014388), respectively, are set forth, in order, in
Table 1 and FIG. 21 (SEQ ID NOs:4-10):
TABLE-US-00001 1:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDELFSDIYKIREIADGLCLEVEGKMVSRTEGAIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDELFSDIYKIREIADGLCLEVEGKMVSRTEGAIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREIAGGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGK-VSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 1:
MIIYRDLISHDEMFSDIYKIREVADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGE 60 61:
GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE 120
61: GTESTVVTGVDIVMNHHLQETSFTKEAYKKYIKDYMKSLKGKLEEQKPERVKPFMTGAAE
120 61:
GTESTVVTGVDIVMNHHLQETSFTKEAYKKYIKDYMKSLKGKLEEQKPERVKPFMTGAAE 120
61: GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE
120 61:
GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE 120
61: GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE
120 61:
GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE 120
61: GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE
120 61:
GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE 120
61: GTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAE
120 121: QIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 172
121: QIKHILANFNNYQFFIGENMNPDGMVALLDYREDGVTPFMIFFKDGLEMEKC 172 121:
QIKHILANFNNYQFFIGENMNPDGMVALLDYREDGVTPFMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFYIGENMNPDGMVALLDYREDGVTPFMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFFIGANMNPDGMVALLDYREDGVTPFMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 172 121:
QIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 172
[0065] Exemplary HRF sequences, that bind to an immunoglobulin (Ig)
include HRF that binds to one or more of IgM, IgG, IgE, IgA, or
IgD. Particular IgE to which HRF binds are associated with immune
disorders and diseases such as those associated with allergies
(food or other antigens), asthma, hypersensitivity reactions and
inflammation.
[0066] As used herein, the terms "peptide," "polypeptide" and
"protein" are used interchangeably and refer to two or more amino
acids covalently linked by an amide bond or equivalent. The
polypeptides of the invention are of any length and include L- and
D-isomers, and combinations of L- and D-isomers. The polypeptides
can include modifications typically associated with
post-translational processing of proteins, for example, cyclization
(e.g., disulfide bond), phosphorylation, glycosylation,
carboxylation, ubiquitination, myristylation, acetylation
(N-terminal), amidation (C-terminal), or lipidation. Polypeptides
described herein further include compounds having amino acid
structural and functional analogues, for example, peptidomimetics
having synthetic or non-natural amino acids or amino acid
analogues, so long as the mimetic has one or more functions or
activities of a native polypeptide set forth herein. Non-natural
and non-amide chemical bonds, and other coupling means can also be
included, for example, glutaraldehyde, N-hydroxysuccinimide esters,
bifunctional maleimides, or N,N'-dicyclohexylcarbodiimide (DCC).
Non-amide bonds can include, for example, ketomethylene
aminomethylene, olefin, ether, thioether and the like (see, e.g.,
Spatola (1983) in Chemistry and Biochemistry of Amino Acids,
Peptides and Proteins, Vol. 7, pp 267-357, "Peptide and Backbone
Modifications," Marcel Decker, N.Y.).
[0067] The term "isolated," when used as a modifier of a
composition (e.g., HRF sequences, antibodies, subsequences,
modified forms, nucleic acids encoding same, etc.), means that the
compositions are made by the hand of man or are separated,
completely or at least in part, from their naturally occurring in
vivo environment. Generally, isolated compositions are
substantially free of one or more materials with which they
normally associate with in nature, for example, one or more
protein, nucleic acid, lipid, carbohydrate, cell membrane. The term
"isolated" does not exclude alternative physical forms of the
composition, such as fusions/chimeras, multimers/oligomers,
modifications (e.g., phosphorylation, glycosylation, lipidation) or
derivatized forms, or forms expressed in host cells produced by the
hand of man.
[0068] An "isolated" composition (e.g., an HRF sequence or
antibody) can also be "substantially pure" or "purified" when free
of most or all of the materials with which it typically associates
with in nature. Thus, an isolated sequence that also is
substantially pure or purified does not include polypeptides or
polynucleotides present among millions of other sequences, such as
antibodies of an antibody library or nucleic acids in a genomic or
cDNA library, for example. Typically, purity can be at least about
50%, 60% or more by mass. The purity can also be about 70% or 80%
or more, and can be greater, for example, 90% or more. Purity can
be determined by any appropriate method, including, for example, UV
spectroscopy, chromatography (e.g., HPLC, gas phase), gel
electrophoresis and sequence analysis (nucleic acid and peptide),
and is typically relative to the amount of impurities, which
typically does not include inert substances, such as water.
[0069] A "substantially pure" or "purified" composition can be
combined with one or more other molecules. Thus, "substantially
pure" or "purified" does not exclude combinations of compositions,
such as combinations of HRF sequences or antibodies, subsequences,
and other antibodies, agents, drugs or therapies.
[0070] As used herein, the term "recombinant," when used as a
modifier of polypeptides, polynucleotides and antibodies, means
that the compositions have been manipulated (i.e., engineered) in a
fashion that generally does not occur in nature (e.g., in vitro). A
particular example of a recombinant polypeptide would be where an
HRF polypeptide or antibody is expressed by a cell transfected with
a polynucleotide encoding the HRF polypeptide or antibody sequence.
A particular example of a recombinant polynucleotide would be where
a nucleic acid (e.g., genomic or cDNA) encoding HRF cloned into a
plasmid, with or without 5', 3' or intron regions that the gene is
normally contiguous with in the genome of the organism. Another
example of a recombinant polynucleotide or polypeptide is a hybrid
or fusion sequence, such as a chimeric HRF or antibody sequence
comprising and a second sequence, such as a heterologous functional
domain.
[0071] The invention also provides antibodies and subsequences
thereof which are useful to bind to or that modulate an HRF
activity or function, or HRF expression. The term "antibody" refers
to a protein that binds to other molecules (antigens) via heavy and
light chain variable domains, V.sub.II and V.sub.L, respectively.
Antibodies include full-length antibodies that include two heavy
and two light chain sequences. Antibodies can have kappa or lambda
light chain sequences, either full length as in naturally occurring
antibodies, mixtures thereof (i.e., fusions of kappa and lambda
chain sequences), and subsequences/fragments thereof. Naturally
occurring antibody molecules contain two kappa or two lambda light
chains.
[0072] In accordance with the invention, there are provided
antibodies and subsequences thereof that bind to a HRF/TCTP
sequence that includes or consists of a region of HRF that binds to
an Ig, such as an IgE. In a particular embodiment, a sequence of
HRF to which antibodies or subsequences thereof bind include or
consist of amino acids 1-19 (MIIYRDLISHDEMFSDIYK (SEQ ID NO:1)) or
amino acids 79-142
(QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNY QFFIGENMNP
(SEQ ID NO:2)) of mammalian HRF. Such antibodies can also bind to
any subsequence of the HRF/TCTP sequence that includes or consists
of a region of HRF that binds to an Ig, such as an IgE. In a
particular embodiment, a subsequence is a portion of amino acids
1-19 (MIIYRDLISHDEMFSDIYK (SEQ ID NO:1)) or a portion of amino
acids 79-142
(QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNY QFFIGENMNP
(SEQ ID NO:2)) of mammalian HRF, or a portion of
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGP
EGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVK
PFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDG LEMEKC (SEQ
ID NO:3), wherein the subsequence is between 5-171 amino acid
residues in length, e.g., 5-10, 10-20, 20-50, 100-150, or 150-171
amino acid residues in length.
[0073] The term "bind," or "binding," when used in reference to an
HRF sequence or antibody, means that the HRF sequence, antibody or
subsequence thereof interacts at the molecular level with an Ig,
such as an IgE, or a corresponding epitope (antigenic determinant)
present on HRF, respectively. Thus, an HRF binds to all or a part
of an Ig sequence, and an antibody specifically binds to all or a
part of sequence or an antigenic epitope on HRF (e.g., an HRF
region that confers binding to an Ig, such as an IgE). Specific
binding is that which is selective for the Ig or HRF. Antibodies
and subsequences thereof include specific or selective binding to
HRF, particularly a region or an epitope within HRF amino acids
1-19 (MIIYRDLISHDEMFSDIYK) or HRF amino acids 79-142
(QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNY
QFFIGENMNP). Specific and selective binding can be distinguished
from non-specific binding using assays known in the art (e.g.,
competition binding, immunoprecipitation, ELISA, flow cytometry,
Western blotting).
[0074] Antibodies of the invention and invention methods employing
antibodies include polyclonal and monoclonal antibodies. The term
"monoclonal," when used in reference to an antibody refers to an
antibody that is based upon, obtained from or derived from a single
clone, including any eukaryotic, prokaryotic, or phage clone. A
"monoclonal" antibody is therefore defined herein structurally, and
not the method by which it is produced.
[0075] Antibodies of the invention and invention methods employing
antibodies can belong to any antibody class, IgM, IgG, IgE, IgA,
IgD, or subclass. Exemplary subclasses for IgG are IgG.sub.1,
IgG.sub.2, IgG.sub.3 and IgG.sub.4.
[0076] Antibodies of the invention and invention methods employing
antibodies include antibody subsequences and fragments. Exemplary
antibody subsequences and fragments include Fab, Fab',
F(ab').sub.2, Fv, Fd, single-chain Fv (scFv), disulfide-linked Fvs
(sdFv), light chain variable region V.sub.L, heavy chain variable
region V.sub.H, trispecific (Fab.sub.3), bispecific (Fab.sub.2),
diabody ((V.sub.L-V.sub.H).sub.2 or (V.sub.H-V.sub.L).sub.2),
triabody (trivalent), tetrabody (tetravalent), minibody
((scF.sub.V-C.sub.H).sub.2), bispecific single-chain Fv (Bis-scFv),
IgGdeltaCH2, scFv-Fc, (scFv).sub.2-Fc and IgG4PE. Such subsequences
and fragments can have the binding affinity as the full length
antibody, the binding specificity as the full length antibody, or
one or more activities or functions of as a full length antibody,
e.g., a function or activity of HRF binding antibody.
[0077] Antibody subsequences and fragments can be combined. For
example, a V.sub.L or V.sub.H subsequences can be joined by a
linker sequence thereby forming a V.sub.L-V.sub.H chimera. A
combination of single-chain Fvs (scFv) subsequences can be joined
by a linker sequence thereby forming a scFv-scFv chimera. Antibody
subsequences and fragments include single-chain antibodies or
variable region(s) alone or in combination with all or a portion of
other subsequences.
[0078] Antibody subsequences and fragments can be prepared by
proteolytic hydrolysis of the antibody, for example, by pepsin or
papain digestion of whole antibodies. Antibody subsequences and
fragments produced by enzymatic cleavage with pepsin provide a 5S
fragment denoted F(ab').sub.2. This fragment can be further cleaved
using a thiol reducing agent to produce 3.5S Fab' monovalent
fragments. Alternatively, an enzymatic cleavage using pepsin
produces two monovalent Fab' fragments and the Fc fragment directly
(see, e.g., U.S. Pat. Nos. 4,036,945 and 4,331,647; and Edelman et
al., Methods Enymol. 1:422 (1967)). Other methods of cleaving
antibodies, such as separation of heavy chains to form monovalent
light-heavy chain fragments, further cleavage of fragments, or
other enzymatic or chemical may also be used.
[0079] Epitopes typically are short amino acid sequences, e.g.
about five to 15 amino acids in length. Epitopes can be contiguous
or non-contiguous. A non-contiguous amino acid sequence epitope
forms due to protein folding. For example, an epitope can include a
non-contiguous amino acid sequence, such as a 5 amino acid sequence
and an 8 amino acid sequence, which are not contiguous with each
other, but form an epitope due to protein folding. Techniques for
identifying epitopes are known to the skilled artisan and include
screening overlapping oligopeptides for binding to antibody (for
example, U.S. Pat. No. 4,708,871), phage display peptide library
kits, which are commercially available for epitope mapping (New
England BioLabs). Epitopes may also be identified by inference when
epitope length peptide sequences are used to immunize animals from
which antibodies that bind to the peptide sequence are obtained and
can be predicted using computer programs, such as BEPITOPE (Odorico
et al., J. Mol. Recognit. 16:20 (2003)).
[0080] Methods of producing polyclonal and monoclonal antibodies
are known in the art. For example, HRF, or a subsequence thereof,
or an immunogenic fragment thereof, optionally conjugated to a
carrier such as keyhole limpet hemocyanin (KLH) or ovalbumin (e.g.,
BSA), or mixed with an adjuvant such as Freund's complete or
incomplete adjuvant, and used to immunize an animal. Using
conventional hybridoma technology, splenocytes from immunized
animals that respond to HRF can be isolated and fused with myeloma
cells. Monoclonal antibodies produced by the hybridomas can be
screened for reactivity with HRF or an immunogenic fragment
thereof.
[0081] Animals that may be immunized include mice, rats, rabbits,
goats, sheep, cows or steer, guinea pigs or primates. Initial and
any optional subsequent immunization may be through intravenous,
intraperitoneal, intramuscular, or subcutaneous routes. Subsequent
immunizations may be at the same or at different concentrations of
HRF, or a subsequence thereof, preparation, and may be at regular
or irregular intervals.
[0082] Human antibodies can be produced by immunizing human
transchromosomic KM Mice.TM. (WO 02/43478) or HAC mice (WO
02/092812). KM Mice.TM. and HAC mice express human immunoglobulin
genes. Using conventional hybridoma technology, splenocytes from
immunized mice that were high responders to the antigen can be
isolated and fused with myeloma cells. A monoclonal antibody can be
obtained that binds to the antigen. An overview of the technology
for producing human antibodies is described in Lonberg and Huszar
(Int. Rev. Immunol. 13:65 (1995)). Transgenic animals with one or
more human immunoglobulin genes (kappa or lambda) that do not
express endogenous immunoglobulins are described, for example in,
U.S. Pat. No. 5,939,598. Additional methods for producing human
polyclonal antibodies and human monoclonal antibodies are described
(see, e.g., Kuroiwa et al., Nat. Biotechnol. 20:889 (2002); WO
98/24893; WO 92/01047; WO 96/34096; WO 96/33735; U.S. Pat. Nos.
5,413,923; 5,625,126; 5,633,425; 5,569,825; 5,661,016; 5,545,806;
5,814,318; 5,885,793; 5,916,771; and 5,939,598).
[0083] Antibodies can also be generated using other techniques
including hybridoma, recombinant, and phage display technologies,
or a combination thereof (see U.S. Pat. Nos. 4,902,614, 4,543,439,
and 4,411,993; see, also Monoclonal Antibodies, Hybridomas: A New
Dimension in Biological Analyses, Plenum Press, Kennett, McKearn,
and Bechtol (eds.), 1980, and Harlow et al., Antibodies: A
Laboratory Manual, Cold Spring Harbor Laboratory Press, 2nd ed.
1988).
[0084] Antibodies of the invention and invention methods employing
antibodies include mammalian, primatized, humanized, fully human
antibodies and chimeras. A mammalian antibody is an antibody
produced by a mammal, transgenic or non-transgenic, or a
non-mammalian organism engineered to produce a mammalian antibody,
such as a non-mammalian cell (bacteria, yeast, insect cell), animal
or plant.
[0085] The term "human" when used in reference to an antibody,
means that the amino acid sequence of the antibody is fully human,
i.e., human heavy and human light chain variable and human constant
regions. Thus, all of the amino acids are human or exist in a human
antibody. An antibody that is non-human may be made fully human by
substituting the non-human amino acid residues with amino acid
residues that exist in a human antibody Amino acid residues present
in human antibodies, CDR region maps and human antibody consensus
residues are known in the art (see, e.g., Kabat, Sequences of
Proteins of Immunological Interest, 4.sup.th Ed. US Department of
Health and Human Services. Public Health Service (1987); Chothia
and Lesk (1987). A consensus sequence of human V.sub.H subgroup
III, based on a survey of 22 known human V.sub.H III sequences, and
a consensus sequence of human V.sub.L kappa-chain subgroup I, based
on a survey of 30 known human kappa I sequences is described in
Padlan Mol. Immunol. 31:169 (1994); and Padlan Mol. Immunol. 28:489
(1991). Human antibodies therefore include antibodies in which one
or more amino acid residues have been substituted with one or more
amino acids present in any other human antibody.
[0086] The term "humanized" when used in reference to an antibody,
means that the amino acid sequence of the antibody has non-human
amino acid residues (e.g., mouse, rat, goat, rabbit, etc.) of one
or more complementarity determining regions (CDRs) that
specifically bind to the desired antigen in an acceptor human
immunoglobulin molecule, and one or more human amino acid residues
in the Fv framework region (FR), which are amino acid residues that
flank the CDRs. Such antibodies typically have reduced
immunogenicity and therefore a longer half-life in humans as
compared to the non-human parent antibody from which one or more
CDRs were obtained or are based upon.
[0087] Antibodies of the invention and invention methods employing
antibodies include those to as "primatized" antibodies, which are
"humanized" except that the acceptor human immunoglobulin molecule
and framework region amino acid residues may be any primate amino
acid residue (e.g., ape, gibbon, gorilla, chimpanzees orangutan,
macaque), in addition to any human residue. Human FR residues of
the immunoglobulin can be replaced with corresponding non-human
residues. Residues in the CDR or human framework regions can
therefore be substituted with a corresponding residue from the
non-human CDR or framework region donor antibody to alter,
generally to improve, antigen affinity or specificity, for example.
A humanized antibody may include residues, which are found neither
in the human antibody nor in the donor CDR or framework sequences.
For example, a FR substitution at a particular position that is not
found in a human antibody or the donor non-human antibody may be
predicted to improve binding affinity or specificity human antibody
at that position. Antibody framework and CDR substitutions based
upon molecular modeling are well known in the art, e.g., by
modeling of the interactions of the CDR and framework residues to
identify framework residues important for antigen binding and
sequence comparison to identify unusual framework residues at
particular positions (see, e.g., U.S. Pat. No. 5,585,089; and
Riechmann et al., Nature 332:323 (1988)).
[0088] The term "chimeric" and grammatical variations thereof, when
used in reference to an antibody, means that the amino acid
sequence of the antibody contains one or more portions that are
derived from, obtained or isolated from, or based upon two or more
different species. For example, a portion of the antibody may be
human (e.g., a constant region) and another portion of the antibody
may be non-human (e.g., a murine heavy or murine light chain
variable region). Thus, an example of a chimeric antibody is an
antibody in which different portions of the antibody are of
different species origins. Unlike a humanized or primatized
antibody, a chimeric antibody can have the different species
sequences in any region of the antibody.
[0089] Antibodies can be humanized using a variety of techniques
known in the art including, for example, CDR-grafting (EP 239,400;
WO91/09967; U.S. Pat. Nos. 5,225,539; 5,530,101; and 5,585,089),
veneering or resurfacing (EP 592,106; EP 519,596; Padlan, Molecular
Immunol. 28:489 (1991); Studnicka et al., Protein Engineering 7:805
(1994); Roguska. et al., Proc. Nat'l. Acad. Sci. USA 91:969
(1994)), and chain shuffling (U.S. Pat. No. 5,565,332). Human
consensus sequences (Padlan, Mol. Immunol. 31:169 (1994); and
Padlan, Mol. Immunol. 28:489 (1991)) have previously used to
produce humanized antibodies (Carter et al., Proc. Natl. Acad. Sci.
USA 89:4285 (1992); and Presta et al., J. Immunol. 151:2623
(1993)).
[0090] Methods for producing chimeric antibodies are known in the
art (e.g., Morrison, Science 229:1202 (1985); Oi et al.,
BioTechniques 4:214 (1986); Gillies et al., J. Immunol. Methods
125:191 (1989); and U.S. Pat. Nos. 5,807,715; 4,816,567; and
4,816,397). Chimeric antibodies in which a variable domain from an
antibody of one species is substituted for the variable domain of
another species are described, for example, in Munro, Nature
312:597 (1984); Neuberger et al., Nature 312:604 (1984); Sharon et
al., Nature 309:364 (1984); Morrison et al., Proc. Nat'l. Acad.
Sci. USA 81:6851 (1984); Boulianne et al., Nature 312:643 (1984);
Capon et al., Nature 337:525 (1989); and Traunecker et al., Nature
339:68 (1989).
[0091] Suitable techniques that additionally may be employed in
antibody methods include affinity purification, non-denaturing gel
purification, HPLC or RP-HPLC, size exclusion, purification on
protein A column, or any combination of these techniques. The
antibody isotype can be determined using an ELISA assay, for
example, a human Ig can be identified using mouse Ig-absorbed
anti-human Ig.
[0092] HRF or a subsequence thereof, suitable for generating
antibodies can be produced by any of a variety of standard protein
purification or recombinant expression techniques known in the art.
For example, HRF or a subsequence thereof can be recombinantly
produced or obtained from cells.
[0093] Forms of protein suitable for generating an immune response
include peptide subsequences of full length protein, such as an
immunogenic fragment. Additional forms of protein include
preparations or cell extracts or fractions, partially purified HRF
or a subsequence thereof, as well as whole cells that express HRF,
or a subsequence thereof, or preparations of HRF or a subsequence
thereof, expressing cells.
[0094] Proteins and antibodies, as well as subsequences and
fragments thereof, can be produced by genetic methodology. Such
techniques include expression of all or a part of the gene encoding
the protein or antibody into a host cell such as Cos cells or E.
coli. The recombinant host cells synthesize full length or a
subsequence, for example, an scFv (see, e.g., Whitlow et al., In:
Methods: A Companion to Methods in Enzymology 2:97 (1991), Bird et
al., Science 242:423 (1988); and U.S. Pat. No. 4,946,778).
Single-chain Fvs and antibodies can be produced as described in
U.S. Pat. Nos. 4,946,778 and 5,258,498; Huston et al., Methods
Enzymol. 203:46 (1991); Shu et al., Proc. Natl. Acad. Sci. USA
90:7995 (1993); and Skerra et al., Science 240:1038 (1988).
[0095] In accordance with the invention, also provided are modified
forms of proteins, antibodies, nucleic acids, and other
compositions, provided that the modified form retains, at least a
part of, a function or activity of the unmodified or reference
protein, nucleic acid, or antibody. For example, a modified HRF
(e.g., a subsequence or fragment) can retain at least partial
binding to an Ig, such as an IgE. In another non-limiting example,
a modified HRF (e.g., a subsequence or fragment) can be used as an
immunogen to produce antibodies that specifically bind to HRF. In
yet another non-limiting example, a modified HRF or HRF binding
antibody (e.g., a subsequence or fragment) can be used in an
invention method.
[0096] The invention therefore includes modified forms of proteins,
antibodies, nucleic acids, and other compositions. Such modified
forms typically retain, at least a part of, one or more functions
or activities of an unmodified or reference protein, nucleic acid,
or antibody. Such activities include, for example, for HRF, binding
to a receptor, such as an Ig, such as an IgE, or modulating HRF
activity, function or expression, etc., and for an HRF antibody,
binding to HRF and inhibiting interactions between HRF and Igs,
such as IgE.
[0097] As used herein, the term "modify" and grammatical variations
thereof, means that the composition deviates from a reference
composition. Such modified proteins, nucleic acids and other
compositions may have greater or less activity or function, or have
a distinct function or activity compared with a reference
unmodified protein, nucleic acid, or composition.
[0098] A "functional polypeptide" or "active polypeptide" refers to
a modified polypeptide that possesses at least one partial function
or biological activity characteristic of a native wild type or full
length counterpart polypeptide as described herein, which can be
identified through an assay. As described herein, a particular
example of a biological activity of HRF is to bind to an Ig, such
as an IgE. Another example of a biological activity is the ability
of an antibody to bind to HRF sequences, such as antibody fragments
that can bind to HRF, such as the sequence region of HRF that
confers binding to an Ig, such as an IgE. Yet another example is an
HRF subsequence that modulates (e.g., decrease, reduce, inhibit,
suppress, limit or control) native (endogenous) HRF activity,
function or expression in vitro, ex vivo or in vivo, presumably by
binding to HRF-R which in turn limits activity or function or
downstream signaling that occurs between native HRF and native
HRF-R.
[0099] Modifications include, for example, substitutions,
additions, insertions and deletions to the amino acid sequences set
forth herein, which can be referred to as "variants." Exemplary
sequence substitutions, additions, and insertions include a full
length or a portion of a sequence with one or more amino acids
substituted, added or inserted, for example of an HRF sequence,
wherein the modified HRF binds to an Ig, such as an IgE, or of an
antibody that binds to HRF, such as the sequence region of HRF that
confers binding to an Ig, such as an IgE.
[0100] Modified polypeptides include, for example, non-conservative
and conservative substitutions of the HRF or antibody amino acid
sequences. In particular embodiments, a modified protein has one or
a few (e.g., 10-20% of the residues of total protein length, or
2-10 residues, substituted) conservative or non-conservative
substitutions.
[0101] As used herein, the term "conservative substitution" denotes
the replacement of an amino acid residue by another, chemically or
biologically similar residue. Biologically similar means that the
substitution does not destroy a biological activity or function,
e.g., HRF binding activity to an Ig, or antibody binding to HRF.
Structurally similar means that the amino acids have side chains
with similar length, such as alanine, glycine and serine, or a
similar size. Chemical similarity means that the residues have the
same charge or are both hydrophilic or hydrophobic. Particular
examples of conservative substitutions include the substitution of
a hydrophobic residue such as isoleucine, valine, leucine or
methionine for another, the substitution of a polar residue for
another, such as the substitution of arginine for lysine, glutamic
for aspartic acids, or glutamine for asparagine, and the like. The
term "conservative substitution" also includes the use of a
substituted amino acid in place of an unsubstituted parent amino
acid. Such proteins that include amino acid substitutions can be
encoded by a nucleic acid. Consequently, nucleic acid sequences
encoding proteins that include amino acid substitutions are also
provided.
[0102] Modified proteins also include one or more D-amino acids
substituted for L-amino acids (and mixtures thereof), structural
and functional analogues, for example, peptidomimetics having
synthetic or non-natural amino acids or amino acid analogues and
derivatized forms. Modifications include cyclic structures such as
an end-to-end amide bond between the amino and carboxy-terminus of
the molecule or intra- or inter-molecular disulfide bond.
[0103] Modified forms further include "chemical derivatives," in
which one or more amino acids has a side chain chemically altered
or derivatized. Such derivatized polypeptides include, for example,
amino acids in which free amino groups form amine hydrochlorides,
p-toluene sulfonyl groups, carobenzoxy groups; the free carboxy
groups form salts, methyl and ethyl esters; free hydroxl groups
that form O-acyl or O-alkyl derivatives as well as naturally
occurring amino acid derivatives, for example, 4-hydroxyproline,
for proline, 5-hydroxylysine for lysine, homoserine for serine,
ornithine for lysine etc. Also included are amino acid derivatives
that can alter covalent bonding, for example, the disulfide linkage
that forms between two cysteine residues that produces a cyclized
polypeptide.
[0104] Modified forms of protein (e.g., HRF, HRF fragment or or
antibody), nucleic acid, and other compositions include additions
and insertions, such as of heterologous domains. For example, an
addition (e.g., heterologous domain) can be the covalent or
non-covalent attachment of any type of molecule to a protein (e.g.,
HRF, HRF fragment or HRF antibody), nucleic acid or other
composition. Typically additions and insertions (e.g., a
heterologous domain) confer a complementary or a distinct function
or activity.
[0105] Additions and insertions include chimeric and fusion
polypeptide or nucleic acid sequences, which is a sequence having
one or more molecules not normally present in a reference native
(wild type) sequence covalently attached to the sequence. The terms
"fusion" or "chimeric" and grammatical variations thereof, when
used in reference to a molecule, such as a HRF, means that a
portions or part of the molecule contains a different entity
distinct (heterologous) from the molecule (e.g., HRF, HRF fragment
or or antibody) as they do not typically exist together in nature.
That is, for example, one portion of the fusion or chimera, such as
HRF, includes or consists of a portion that does not exist together
in nature, and is structurally distinct. A particular example is a
molecule, such as an amino acid sequence of another protein (e.g.,
immunoglobulin such as an Fc domain, or antibody) attached to HRF
to produce a chimera, or a chimeric polypeptide, to impart a
distinct function (e.g., increased solubility, in vivo half life,
etc.). Another particular example is an amino acid sequence of
another protein to produce a multifunctional protein (e.g.,
multifunctional HRF or multispecific antibody).
[0106] Additions and insertions also include label or a tag, which
can be used to provide an agent that is detectable or that is
usefule for isolating the tagged entitiy (e.g., HRF, HRF fragment
or HRF antibody). A detectable label can be attached, for example,
to (e.g., linked conjugated) HRF, HRF fragment or HRF antibody, or
be within or be one or more atoms that comprise the molecule.
[0107] Non-limiting exemplary detectable labels also include a
radioactive material, such as a radioisotope, a metal or a metal
oxide. Radioisotopes include radionuclides emitting alpha, beta or
gamma radiation. In particular embodiments, a radioisotope can be
one or more of: .sup.3H, .sup.10B, .sup.18F, .sup.11C, .sup.14C,
.sup.13N, .sup.18O, .sup.15O, .sup.32P, P.sup.33, .sup.35S,
.sup.35Cl, .sup.45Ti, .sup.46Sc, .sup.47Sc, .sup.51Cr, .sup.52Fe,
.sup.59Fe, .sup.57Co, .sup.60Cu, .sup.61Cu, .sup.62Cu, .sup.64Cu,
.sup.67Cu, .sup.67Ga, .sup.68Ga, .sup.72As .sup.76Br, .sup.77Br,
.sup.81mKr, .sup.82Rb, .sup.85Sr, .sup.89Sr, .sup.86Y, .sup.90Y,
.sup.95Nb, .sup.94mTc, .sup.99mTc, .sup.97Ru, .sup.103Ru,
.sup.105Rh, .sup.109Cd, .sup.111In, .sup.113Sn, .sup.113mIn,
.sup.114In, I.sup.125, I.sup.131, .sup.140La, .sup.141Ce,
.sup.149Pm, .sup.153Gd, .sup.157Gd, .sup.153Sm, .sup.161Tb,
.sup.166Dy, .sup.166Ho, .sup.169Er, .sup.169Y, .sup.175Yb,
.sup.177Lu, .sup.186Re, .sup.188Re, .sup.201Tl, .sup.203Pb,
.sup.211At, .sup.212Bi or .sup.225Ac.
[0108] Additional non-limiting exemplary detectable labels include
a metal or a metal oxide. In particular embodiments, a metal or
metal oxide is one or more of: gold, silver, copper, boron,
manganese, gadolinium, iron, chromium, barium, europium, erbium,
praseodymium, indium, or technetium. In additional embodiments, a
metal oxide includes one or more of: Gd(III), Mn(II), Mn(III),
Cr(II), Cr(III), Cu(II), Fe (III), Pr(III), Nd(III) Sm(III),
Tb(III), Yb(III) Dy(III), Ho(III), Eu(II), Eu(III), or Er(III).
[0109] Further non-limiting exemplary detectable labels include
contrast agents (e.g., gadolinium; manganese; barium sulfate; an
iodinated or noniodinated agent; an ionic agent or nonionic agent);
magnetic and paramagnetic agents (e.g., iron-oxide chelate);
nanoparticles; an enzyme (horseradish peroxidase, alkaline
phosphatase, .beta.-galactosidase, or acetylcholinesterase); a
prosthetic group (e.g., streptavidin/biotin and avidin/biotin); a
fluorescent material (e.g., umbelliferone, fluorescein, fluorescein
isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein,
dansyl chloride or phycoerythrin); a luminescent material (e.g.,
luminol); or a bioluminescent material (e.g., luciferase,
luciferin, aequorin).
[0110] Additional non-limiting examples of tags and/or detectable
labels include enzymes (horseradish peroxidase, urease, catalase,
alkaline phosphatase, beta-galactosidase, chloramphenicol
transferase); enzyme substrates; ligands (e.g., biotin); receptors
(avidin); GST-, T7-, His-, myc-, HA- and FLAG-tags; electron-dense
reagents; energy transfer molecules; paramagnetic labels;
fluorophores (fluorescein, fluorscamine, rhodamine, phycoerthrin,
phycocyanin, allophycocyanin); chromophores; chemi-luminescent
(imidazole, luciferase, acridinium, oxalate); and bio-luminescent
agents.
[0111] As set forth herein, a detectable label or tag can be linked
or conjugated (e.g., covalently) to the molecule (e.g., HRF, HRF
fragment or antobidy). In various embodiments a detectable label,
such as a radionuclide or metal or metal oxide can be bound or
conjugated to the agent, either directly or indirectly. A linker or
an intermediary functional group can be used to link the molecule
to a detectable label or tag. Linkers include amino acid or
peptidomimetic sequences inserted betweenthe molecule and a label
or tag so that the two entities maintain, at least in part, a
distinct function or activity. Linkers may have one or more
properties that include a flexible conformation, an inability to
form an ordered secondary structure or a hydrophobic or charged
character which could promote or interact with either domain. Amino
acids typically found in flexible protein regions include Gly, Asn
and Ser. The length of the linker sequence may vary without
significantly affecting a function or activity.
[0112] Linkers further include chemical moieties, conjugating
agents, and intermediary functional groups. Examples include
moieties that react with free or semi-free amines, oxygen, sulfur,
hydroxy or carboxy groups. Such functional groups therefore include
mono and bifunctional crosslinkers, such as sulfo-succinimidyl
derivatives (sulfo-SMCC, sulfo-SMPB), in particular, disuccinimidyl
suberate (DSS), BS3 (Sulfo-DSS), disuccinimidyl glutarate (DSG) and
disuccinimidyl tartrate (DST). Non-limiting examples include
diethylenetriaminepentaacetic acid (DTPA) and ethylene
diaminetetracetic acid.
[0113] Additional non-limiting examples of amino acid modifications
include protein subsequences and fragments. Exemplary HRF
subsequences and fragments include a portion of the HRF sequence
that binds to an Ig, such as an IgE. Exemplary HRF subsequences and
fragments also include an immunogenic portion of HRF.
[0114] As used herein, the term "subsequence" or "fragment" means a
portion of the full length molecule. A subsequence of a polypeptide
sequence, such as HRF or an antibody sequence, has one or more less
amino acids than a full length HRF (e.g. one or more internal or
terminal amino acid deletions from either amino or
carboxy-termini). A subsequence of an antibody has one or more less
amino acids than a full length antibody heavy or light chain or
constant region. A nucleic acid subsequence has at least one less
nucleotide than a full length comparison nucleic acid sequence.
Subsequences therefore can be any length up to the full length
native molecule.
[0115] Functional subsequences can vary in size from a polypeptide
as small as an epitope capable of binding an antibody molecule
(i.e., about five amino acids) up to the entire length of a
reference polypeptide. Functional HRF subsequences are at least
5-10, 10-20, 20-30, 30-40, 40-50, 50-75, 75-100, 100-150, or
150-171 amino acid residues. Functional antibody subsequences are
at least 5-10, 10-20, 20-30, 30-40, 40-50, 50-75, 75-100, 100-125
amino acid residues.
[0116] Thus, in another embodiment, the invention provides
functional polypeptides or functional subsequences thereof. In
particular embodiments, a functional polypeptide or functional
subsequence shares at least 50% identity with a reference sequence,
for example, an HRF polypeptide sequence and that binds to an Ig,
such as an IgE, or is capable of modulating HRF activity, function
or expression, or an antibody that binds to the HRF sequence region
that mediates HRF binding to an Ig, such as an IgE. In other
embodiments, the polypeptides have at least 60%, 70%, 75% or more
identity (e.g., 80%, 85% 90%, 95%, 96%, 97%, 98%, 99% or more
identity) to a reference sequence, such as an HRF polypeptide
sequence that binds to an Ig, such as an IgE, or is capable of
modulating HRF activity, function or expression. The functional
polypeptides or functional subsequences thereof including modified
forms of the invention, such as HRF and antibodies that bind to
HRF, may have one or more of the functions or biological activities
described herein.
[0117] The term "identity" and grammatical variations thereof, mean
that two or more referenced entities are the same. Thus, where two
polypeptide (e.g., HRF or antibody) sequences are identical, they
have the same amino acid sequence, at least within the referenced
region or portion. Where two nucleic acid sequences are identical,
they have the same polynucleotide sequence, at least within the
referenced region or portion. The identity can be over a defined
area (region or domain) of the sequence. An "area of identity"
refers to a portion of two or more referenced entities that are the
same. Thus, where two protein or nucleic acid sequences are
identical over one or more sequence regions they share identity
within that region.
[0118] The percent identity can extend over the entire sequence
length of the polypeptide (e.g., HRF). In particular aspects, the
length of the sequence sharing the percent identity is 5 or more
contiguous amino acids, e.g., 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,
15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, etc. contiguous amino
acids. In additional particular aspects, the length of the sequence
sharing the percent identity is 25 or more contiguous amino acids,
e.g., 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, etc. contiguous amino
acids. In further particular aspects, the length of the sequence
sharing the percent identity is 35 or more contiguous amino acids,
e.g., 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 45, 47, 48, 49,
50, etc., contiguous amino acids. In yet additional particular
aspects, the length of the sequence sharing the percent identity is
50 or more contiguous amino acids, e.g., 50-55, 55-60, 60-65,
65-70, 70-75, 75-80, 80-85, 85-90, 90-95, 95-100, 100-110, etc.
contiguous amino acids.
[0119] The terms "homologous" or "homology" mean that two or more
referenced entities share at least partial identity over a given
region or portion. "Areas, regions or domains" of homology or
identity mean that a portion of two or more referenced entities
share homology or are the same. Thus, where two sequences are
identical over one or more sequence regions they share identity in
these regions. "Substantial homology" means that a molecule is
structurally or functionally conserved such that it has or is
predicted to have at least partial structure or function of one or
more of the structures or functions (e.g., a biological function or
activity) of the reference molecule, or relevant/corresponding
region or portion of the reference molecule to which it shares
homology. An HRF sequence or an antibody or subsequence with
substantial homology has or is predicted to have at least partial
activity or function as the reference HRF sequence or antibody.
[0120] The extent of identity (homology) between two sequences can
be ascertained using a computer program and mathematical algorithm
known in the art. Such algorithms that calculate percent sequence
identity (homology) generally account for sequence gaps and
mismatches over the comparison region or area. For example, a BLAST
(e.g., BLAST 2.0) search algorithm (see, e.g., Altschul et al., J.
Mol. Biol. 215:403 (1990), publicly available through NCBI) has
exemplary search parameters as follows: Mismatch -2; gap open 5;
gap extension 2. For polypeptide sequence comparisons, a BLASTP
algorithm is typically used in combination with a scoring matrix,
such as PAM100, PAM 250, BLOSUM 62 or BLOSUM 50. FASTA (e.g.,
FASTA2 and FASTA3) and SSEARCH sequence comparison programs are
also used to quantitate extent of identity (Pearson et al., Proc.
Natl. Acad. Sci. USA 85:2444 (1988); Pearson, Methods Mol. Biol.
132:185 (2000); and Smith et al., J. Mol. Biol. 147:195 (1981)).
Programs for quantitating protein structural similarity using
Delaunay-based topological mapping have also been developed
(Bostick et al., Biochem Biophys Res Commun. 304:320 (2003)).
[0121] Modifications can be produced using methods known in the art
(e.g., PCR based site-directed, deletion and insertion mutagenesis,
chemical modification and mutagenesis, cross-linking, etc.), or may
be spontaneous or naturally occurring (e.g. random mutagenesis).
For example, naturally occurring allelic variants can occur by
alternative RNA splicing, polymorphisms, or spontaneous mutations
of a nucleic acid encoding HRF polypeptide. Further, deletion of
one or more amino acids can also result in a modification of the
structure of the resultant polypeptide without significantly
altering a biological function or activity. Deletion of amino acids
can lead to a smaller active molecule. For example, as set forth
herein, removal of HRF amino or carboxy terminal or internal amino
acids does not destroy Ig binding activity.
[0122] Modified HRF sequences, antibodies and subsequences fragment
of the invention may have an affinity greater or less than 2-5,
5-10, 10-100, 100-1000 or 1000-10,000-fold affinity, or any
numerical value or range within or encompassing such values, than a
comparison HRF sequence or antibody. In one embodiment, an HRF
sequence has a binding affinity for an Ig, such as an IgE, within
about 1-5000 fold of the binding affinity of HRF amino acids 1-19
(MIIYRDLISHDEMFSDIYK (SEQ ID NO:1)) or HRF amino acids 79-142
(QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNY QFFIGENMNP
(SEQ ID NO:2)), for binding to an Ig, such as an IgE.
[0123] The term "substantially the same" when used in reference to
an HRF sequence or subsequence thereof that binds to an Ig, or an
antibody or subsequence thereof that binds to HRF, means that the
relative binding affinity or avidity for binding to an Ig, such as
an IgE, means that the binding is within 100 fold (greater or less
than) of the binding affinity of a reference HRF sequence or
antibody (e.g., an Ig, such as an IgE, or HRF). Binding affinity
can be determined by association (K.sub.a) and dissociation
(K.sub.D or K.sub.d) rate. Equilibrium affinity constant, K, is the
ratio of K.sub.a/K.sub.d. Association (K.sub.a) and dissociation
(K.sub.D or K.sub.d) rates can be measured using surface plasmon
resonance (SPR) (Rich and Myszka, Curr. Opin. Biotechnol. 11:54
(2000); Englebienne, Analyst. 123:1599 (1998)). Instrumentation and
methods for real time detection and monitoring of binding rates are
known and are commercially available (BiaCore 2000, Biacore AB,
Upsala, Sweden; and Malmqvist, Biochem. Soc. Trans. 27:335 (1999)).
Thus, for example, if binding of a reference HRF antibody to HRF
has a K.sub.D of 10.sup.-9 M, then an antibody which has
substantially the same binding affinity as the reference antibody
will have a K.sub.D within the range of 10.sup.-7 M to K.sub.D
10.sup.-11 M for binding to HRF.
[0124] The invention also provides polynucleotides encoding HRF
polypeptides and antibodies that bind to HRF. In one embodiment, a
polynucleotide sequence has about 65% or more identity (e.g., 70%,
75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) to a sequence
encoding an HRF subsequence that binds to an Ig, such as an IgE. In
particular embodiments, a nucleic acid encodes all or a portion of
amino acids 1-19 (MIIYRDLISHDEMFSDIYK (SEQ ID NO:1)) or amino acids
79-142 (QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNY
QFFIGENMNP (SEQ ID NO:2)) of mammalian HRF. Such polynucleotides
can therefore encode any subsequence of the HRF/TCTP sequence that
includes or consists of a region of HRF that binds to an Ig, such
as an IgE. Such encoded subsequences can be between 5-171 amino
acid residues in length, e.g., 5-10, 10-20, 20-50, 100-150, or
150-171 amino acid residues in length.
[0125] As used herein, the terms "polynucleotide" and "nucleic
acid" are used interchangeably to refer to all forms of nucleic
acid, oligonucleotides, primers, and probes, including
deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Polynucleotides include genomic DNA, cDNA and antisense DNA, and
spliced or unspliced mRNA, rRNA tRNA and antisense RNA (e.g.,
RNAi). Polynucleotides include naturally occurring, synthetic, and
intentionally altered or modified polynucleotides as well as
analogues and derivatives. Alterations can result in increased
stability due to resistance to nuclease digestion, for example.
Polynucleotides can be double, single or triplex, linear or
circular, and can be of any length.
[0126] Polynucleotides of the invention include sequences that are
degenerate as a result of the genetic code. There are 20 natural
amino acids, most of which are specified by more than one codon.
Degenerate sequences may not selectively hybridize to other
invention nucleic acids; however, they are nonetheless included as
they encode invention HRF polypeptides and modified forms including
subsequences thereof. Thus, in another embodiment, degenerate
nucleotide sequences that encode HRF polypeptides and modified
forms including subsequences thereof as set forth herein, are
provided.
[0127] Polynucleotide sequences include sequences having 15-20,
20-30, 30-40, 50-50, or more contiguous nucleotides. In additional
aspects, the polynucleotide sequence includes a sequence having 60
or more, 70 or more, 80 or more, 100 or more, 120 or more, 140 or
more, 160 or more contiguous nucleotides, up to the full length
coding sequence.
[0128] Polynucleotide sequences for HRF include complementary
sequences (e.g., antisense to all or a part of HRF). Antisense
polynucleotides, to decrease activity, function or expression of
HRF, for example, do not require expression control elements to
function in vivo. However, antisense may be encoded by a nucleic
acid and such a nucleic acid may be operatively linked to an
expression control element for sustained or increased expression of
the encoded antisense in cells or in vivo. Sequences encoding HRF
subsequences that bind to an Ig, such as an IgE also are included.
Such HRF forms may decrease, reduce, inhibit, suppress, limit or
control binding or interaction of the native endogenous HRF with
HRF-R thereby modulating signaling.
[0129] Further included are double stranded RNA sequences from an
HRF coding region. The use of double stranded RNA sequences (known
as "RNAi") for inhibiting gene expression, for example, in insects
and in other organisms is known in the art (Kennerdell et al., Cell
95:1017 (1998); Fire et al., Nature, 391:806 (1998)).
[0130] Such complementary, antisense and RNAi sequences can
interfere with HRF activity, function or expression and be useful
for modulating HRF. An effective amount of complementary, antisense
or RNAi sequences from the coding region of HRF can inhibit HRF
activity, function or expression and are therefore useful in the
therapeutic and other methods of treatment as described herein.
Such invention polynucleotides can be further contained within
carriers or vectors suitable for passing through a cell membrane
for cytoplasmic delivery, and can be modified so as to be nuclease
resistant in order to enhance their stability or efficacy in the
invention methods and compositions, for example.
[0131] Thus, in another embodiment, polynucleotides encoding HRF
including the nucleotide sequence encoding full length or a
subsequence of:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGP
EGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVK
PFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDG LEMEKC, (SEQ
ID NO:3) as well as nucleic acid sequences complementary to the
sequence or subsequence (e.g., complementary, antisense
polynucleotides), are provided.
[0132] Polynucleotides encoding full length or subsequences of HRF
polypeptide are included herein. Particular examples are nucleic
acid sequences that encode HRF functional subsequences. As used
herein, the term "functional polynucleotide" denotes a
polynucleotide that encodes a functional polypeptide as described
herein. Thus, the invention includes polynucleotides encoding a
polypeptide having a function or activity of an amino acid sequence
set forth in HRF, e.g.,
TABLE-US-00002 MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLI
GGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYM
KSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDG
MVALLDYREDGVTPYMIFFKDGLEMEKC.
[0133] Additional polynucleotides include fragments of the
above-described nucleic acid sequences that are at least 15 bases
in length, which is of sufficient length to permit a selective
hybridization to an HRF nucleic acid. Polynucleotide fragments of
at least 15 bases in length can be used to screen for HRF related
genes in other organisms, such as mammals or insects, and are
referred to herein as "probes."
[0134] Invention probes and agents additionally can have a "tag" or
"label" or "detectable moiety" linked thereto that provides a means
of isolation or identification, or a detection signal (e.g.,
radionuclides, fluorescent, chemi- or other luminescent moieties).
If necessary, additional reagents can be used in combination with
the detectable moieties to provide or enhance the detection signal.
Such labels and detectable moieties also can be linked to invention
HRF polypeptides, nucleic acids, antibodies, and modified forms
disclosed herein.
[0135] Thus, in accordance with the invention there are provided
isolated polynucleotides that selectively hybridize to the
polynucleotides described herein. In one embodiment, an isolated
polynucleotide sequence hybridizes under stringent conditions to a
polynucleotide encoding full length or a subsequence of HRF, e.g.,
encoding all or a subsequence of:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGP
EGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVK
PFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDG LEMEKC (SEQ
ID NO:3). In another embodiment, an isolated polynucleotide
sequence hybridizes under stringent conditions to a polynucleotide
encoding full length or a subsequence of HRF sequence set forth
herein.
[0136] Hybridization refers to binding between complementary
nucleic acid sequences (e.g., sense/antisense). As used herein, the
term "selective hybridization" refers to hybridization under
moderately stringent or highly stringent conditions, which can
distinguish HRF related nucleotide sequences from unrelated
sequences. Screening procedures which rely on hybridization allow
isolation of related nucleic acid sequences, from any organism.
[0137] In nucleic acid hybridization reactions, conditions used in
order to achieve a particular level of stringency will vary,
depending on the nature of the nucleic acids being hybridized. For
example, the length, degree of sequence complementarity, sequence
composition (e.g., the GC v. AT content), and type (e.g., RNA v.
DNA) of the hybridizing regions can be considered in selecting
particular hybridization conditions. An additional consideration is
whether one of the nucleic acids is immobilized, for example, on a
filter. As is understood by those skilled in the art, the Tm
(melting temperature) refers to the temperature at which the
binding between two sequences is no longer stable. For two
sequences to form a stable hybrid, the temperature of the reaction
must be less than the Tm for the particular hybridization
conditions. In general, the stability of a nucleic acid hybrid
decreases as the sodium ion decreases and the temperature of the
hybridization reaction increases.
[0138] Typically, wash conditions are adjusted so as to attain the
desired degree of stringency. Thus, hybridization stringency can be
determined, for example, by washing at a particular condition,
e.g., at low stringency conditions or high stringency conditions,
or by using each of the conditions, e.g., for 10-15 minutes each,
in the order listed below, repeating any or all of the steps
listed. Optimal conditions for selective hybridization will vary
depending on the particular hybridization reaction involved.
[0139] An example of a moderately stringent hybridization condition
is as follows: 2.times.SSC/0.1% SDS at about 37.degree. C. or
42.degree. C. (hybridization conditions); 0.5.times.SSC/0.1% SDS at
about room temperature (low stringency wash); 0.5.times.SSC/0.1%
SDS at about 42.degree. C. (moderate stringency wash). An example
of a moderately-high stringent hybridization condition is as
follows: 2.times.SSC/0.1% SDS at about 37.degree. C. or 42.degree.
C. (hybridization conditions); 0.5.times.SSC/0.1% SDS at about room
temperature (low stringency wash); 0.5.times.SSC/0.1% SDS at about
42.degree. C. (moderate stringency wash); and 0.1.times.SSC/0.1%
SDS at about 52.degree. C. (moderately-high stringency wash). An
example of high stringency hybridization conditions is as follows:
2.times.SSC/0.1% SDS at about room temperature (hybridization
conditions); 0.5.times.SSC/0.1% SDS at about room temperature (low
stringency wash); 0.5.times.SSC/0.1% SDS at about 42.degree. C.
(moderate stringency wash); and 0.1.times.SSC/0.1% SDS at about
65.degree. C. (high stringency wash).
[0140] Polynucleotides of the invention can be obtained using
various standard cloning and chemical synthesis techniques. Purity
of polynucleotides can be determined through sequencing, gel
electrophoresis and the like. For example, nucleic acids can be
isolated using hybridization as set forth herein or computer-based
database screening techniques known in the art. Such techniques
include, but are not limited to: (1) hybridization of genomic DNA
or cDNA libraries with probes to detect homologous nucleotide
sequences; (2) antibody screening to detect polypeptides having
shared structural features, for example, using an expression
library; (3) polymerase chain reaction (PCR) on genomic DNA or cDNA
using primers capable of annealing to a nucleic acid sequence of
interest; (4) computer searches of sequence databases for related
sequences; and (5) differential screening of a subtracted nucleic
acid library.
[0141] The invention HRF polynucleotides can include an expression
control element distinct from the endogenous HRF gene (e.g., a
non-native element), or exclude a control element from the native
HRF gene to control expression of an operatively linked HRF nucleic
acid. Such polynucleotides containing an expression control element
controlling expression of a nucleic acid can be modified or altered
as set forth herein, so long as the modified or altered
polynucleotide has one or more functions or activities.
[0142] For expression in cells, invention polynucleotides, if
desired, may be inserted into a vector. Accordingly, invention
compositions and methods further include polynucleotide sequences
inserted into a vector. The term "vector" refers to a plasmid,
virus or other vehicle known in the art that can be manipulated by
insertion or incorporation of a polynucleotide. Such vectors can be
used for genetic manipulation (i.e., "cloning vectors") or can be
used to transcribe or translate the inserted polynucleotide (i.e.,
"expression vectors"). A vector generally contains at least an
origin of replication for propagation in a cell and a promoter.
Control elements, including expression control elements as set
forth herein, present within a vector are included to facilitate
proper transcription and translation (e.g., splicing signal for
introns, maintenance of the correct reading frame of the gene to
permit in-frame translation of mRNA and, stop codons etc.).
[0143] Compositions and methods of the invention are applicable to
treating numerous disorders. Disorders treatable in accordance with
the invention include disorders in which decreasing, reducing,
inhibiting, suppressing, limiting or controlling a response
mediated or associated with HRF activity, function or expression
can provide a subject with a benefit. Disorders include undesirable
or aberrant immune responses, immune disorders and immune diseases
including, for example, food allergy, allergic reaction,
hypersensitivity, inflammatory response, inflammation, and airway
constriction.
[0144] As used herein, an "undesirable immune response" or
"aberrant immune response" refers to any immune response, activity
or function that is greater or less than desired or physiologically
normal. An undesirable immune response, function or activity can be
a normal response, function or activity. Thus, normal immune
responses so long as they are undesirable, even if not considered
aberrant, are included within the meaning of these terms. An
undesirable immune response, function or activity can also be an
abnormal response, function or activity. An abnormal (aberrant)
immune response, function or activity deviates from normal.
Undesirable and aberrant immune responses can be humoral,
cell-mediated or a combination thereof, either chronic or
acute.
[0145] One non-limiting example of an undesirable or aberrant
immune response is where the immune response is hyper-responsive,
such as in the case of an autoimmune disorder or disease. Another
example of an undesirable or aberrant immune response is where an
immune response leads to acute or chronic inflammatory response or
inflammation in any tissue or organ, such as an allergy (e.g., food
allergy or asthma).
[0146] The terms "immune disorder" and "immune disease" mean, an
immune function or activity, that is greater than (e.g.,
autoimmunity) or less than (e.g., immunodeficiency) desired, and
which is characterized by different physiological symptoms or
abnormalities, depending upon the disorder or disease. Particular
non-limiting examples of immune disorders and diseases to which the
invention applies include, for example, food allergy, allergic
reaction, hypersensitivity, inflammatory response, inflammation,
and airway constriction. Additional disorders are generally
characterized as an undesirable or aberrant increased or
inappropriate response, activity or function of the immune system.
Disorders and diseases that can be treated in accordance with the
invention include, but are not limited to, disorders and disease
that cause cell or tissue/organ damage in the subject.
[0147] In accordance with the invention, there are provided methods
of treating a food allergy. In one embodiment, a method includes
contacting histamine releasing factor (HRF)/translationally
controlled tumor protein (TCTP) with a compound that inhibits or
reduces binding of HRF/TCTP to an immunoglobulin thereby treating
the food allergy.
[0148] As used herein, the term "food allergy" refers to an adverse
immune response to food proteins, which is unlike lactose
intolerance, food poisoning or food aversions. A particular type of
food allergy is a "rapid" type of food allergy, often called as
"IgE-mediated" food hypersensitivity. Symptoms of food allergy may
include systemic anaphylaxis (e.g., hypotension, loss of
consciousness, and death), skin (e.g., flushing, urticaria,
angioedema, and worsening eczema), eye (e.g., allergic
conjunctivitis), gut (e.g., nausea, cramping, vomiting, diarrhea,
and abdominal pain), and respiratory tract reactions (e.g.,
rhinitis and asthma) (Sicherer et al., Pediatrics 102:e6 (1998);
Atkins et al., J. Allergy Clin. Immunol. 75:356 (1985); Lack, N.
Engl. J. Med. 359:1252 (2008)). Examples of known food allergens
include peanuts, tree nuts, fish, and shellfish are common
allergens in both children and adults, while children also often
react to eggs, wheat, and soy.
[0149] Food-induced allergic reactions result from immunologic
pathways that include activation of effector cells through food
specific IgE antibodies, cell-mediated (non-IgE-mediated) reactions
resulting in subacute or chronic inflammation, or the combination
of these pathways. The significance of IgE-mediated arm of
reactions in human was demonstrated by anti-IgE therapy in patients
with peanut allergy, which significantly and substantially
increased the threshold of sensitivity to peanut on oral food
challenge (Leung et al., N. Engl. J. Med. 348:986 (2003)).
Furthermore, histamine has been reported to increase in food
allergy patients after allergen challenge (Sampson and Jolie, N.
Engl. J. Med. 311:372 (1984)), suggesting involvement of mast cell
or basophil activation downstream of IgE-mediated pathways. On the
other hand, celiac disease, which is a representative of the
cell-mediated arm of food hypersensitivity, is mediated by
gluten-reactive T cells, and the symptoms are confined to gut,
often mild and chronic (Sollid and Lundin, Mucosal Immunol. 2:3
(2009)).
[0150] Consequently, methods of the invention include modulating
(e.g., decrease, reduce, inhibit, suppress, limit or control)
immunologic pathways that include activation of effector cells
through food specific IgE antibodies, cell-mediated
(non-IgE-mediated) reactions resulting in subacute or chronic
inflammation, or a combination of these pathways. Methods of the
invention also include modulating (e.g., decrease, reduce, inhibit,
suppress, limit or control) IgE-mediated reactions thereby
treating, inhibiting, reducing or decreasing sensitivity to food
allergies. Methods of the invention further include modulating
(e.g., decrease, reduce, inhibit, suppress, limit or control)
histamine release or activation of downstream IgE-mediated
pathways, such as by, but not limited to, mast cells or
basophils.
[0151] As disclosed herein, animal models indicate that food
allergy may be elicited by classic or alternative pathways, both of
which appear to depend on immunoglobulins (Igs). Methods of the
invention therefore also include modulating (e.g., decrease,
reduce, inhibit, suppress, limit or control) either or both
pathways that appear to contribute to food allergy. Such methods
include, for example, modulating (e.g., decrease, reduce, inhibit,
suppress, limit or control) B cells or mast cells, or release of
histamine and platelet activating factor (PAF), which is believed
to cause anaphylactic symptoms. Such methods also include, for
example, modulating (e.g., decrease, reduce, inhibit, suppress,
limit or control) hypothermia.
[0152] As also disclosed herein, animal models indicate that the
Ig/mast cell axis plays a role in food allergy. The food-induced
anaphylaxis and diarrhea models reveal the role of B cells and mast
cells, and a contributory role of Fc.epsilon.RI, suggesting a
central role of Ig/mast cell interaction in the effecter phase of
food allergy. As disclosed herein, a subset of IgE and IgG
molecules have been identified as HRF receptors, and the HRF
reactivity of Igs could modulate the effecter phase of food
allergy.
[0153] Methods of the invention therefore also include modulating
numbers or activity of mast cells or B cells in the small
intestine, such as in the jejunum, or in the colon (large
intestine). Such methods include, for example, decreasing,
reducing, inhibiting, suppressing, limiting or controlling numbers
or activity of mast cells or B cells in the small intestine, such
as in the jejunum. Such methods also include, for example,
inhibiting, reducing or decreasing numbers or activity of mast
cells or B cells in the colon (large intestine). Such methods
further include treating diarrhea.
[0154] As further disclosed herein, food allergy is prevalent in
patients with eosinophilic esophagitis (EoE), and HRF appears to
play a role in food allergy or the underlying eosinophilic
esophagitis (EoE). HRF therefore plays a role in food allergy and
EoE.
[0155] Methods of the invention are therefore applicable to
treatment of food allergy in subjects with eosinophilic esophagitis
(EoE). Methods of the invention therefore include treatment (e.g.,
decrease, reduce, inhibit, suppress, limit or control) of one or
more symptoms, such as vomiting, abdominal pain, or failure to
thrive, for example, in young children, or, for example, dysphagia
in adolescents or adults. Methods of the invention also include
treatment (e.g., decrease, reduce, inhibit, suppress, limit or
control) of esophageal stricture formation and tissue remodeling.
Methods of the invention further include treatment of (e.g.,
decrease, reduce, inhibit, suppress, limit or control) esophageal
dysmotility in a subject, such as in an adult or pediatric EoE
subject. Methods of the invention additionally include treatment of
(e.g., decrease, reduce, inhibit, suppress, limit or control) local
IgE production and systemic sensitization that occur in EoE.
Methods of the invention moreover include treatment (e.g.,
decrease, reduce, inhibit, suppress, limit or control) of delayed
type hypersensitivity and esophageal mastocytosis, such as in
patients suffering from EoE (Kirsh et al., J. Pediatric
Gastroenterol. Nutrition 44:20 (2007)).
[0156] The term "contacting" means direct or indirect binding or
interaction between two or more entities (e.g., between an HRF
sequence and native endogenous HRF, or between an antibody and
endogenous HRF). A particular example of direct interaction is
binding. A particular example of an indirect interaction is where
one entity acts upon an intermediary molecule, which in turn acts
upon the second referenced entity. Thus, for example, contacting
HRF with an antibody includes allowing the antibody to bind to HRF,
or allowing the antibody to act upon an intermediary that in turn
binds to HRF. Contacting as used herein includes in solution, in
solid phase, in vitro, ex vivo, in a cell and in vivo. Contacting
in vivo can be referred to as administering, or administration.
[0157] In accordance with the invention, there are provided methods
of treating an allergic reaction, hypersensitivity, an inflammatory
response or inflammation. In one embodiment, a method includes
contacting histamine releasing factor (HRF)/translationally
controlled tumor protein (TCTP) with a compound that inhibits or
reduces binding of HRF/TCTP to an immunoglobulin thereby treating
the allergic reaction, hypersensitivity, inflammatory response or
inflammation.
[0158] In accordance with the invention, there are also provided
methods for decreasing, reducing, inhibiting, suppressing, limiting
or controlling the probability, severity, frequency, duration or
preventing a subject from having an acute or chronic food allergy,
allergic reaction, hypersensitivity, an inflammatory response or
inflammation. In one embodiment, a method includes administering to
a subject a compound that decreass, reduces, inhibits, suppresses,
limits or controls binding of HRF/TCTP to an immunoglobulin thereby
decreasing, reducing, inhibiting, suppressing, limiting or
controlling the probability, severity, frequency, duration or
preventing the subject from having an acute or chronic food
allergy, allergic reaction, hypersensitivity, inflammatory response
or inflammation.
[0159] Methods of the invention include modulating (e.g., decrease,
reduce, inhibit, suppress, limit or control) one or more functions,
activities or expression of HRF, in vitro, ex vivo or in vivo. As
used herein, the term "modulate," means an alteration or effect of
the term modified. For example, the term modulate can be used in
various contexts to refer to an alteration or effect of an
activity, a function, or expression of a polypeptide, gene or
signaling pathway, or a physiological condition or response of an
organism. Thus, where the term "modulate" is used to modify the
term "HRF" this means that an HRF activity, function, or expression
is altered or affected (e.g., decreased, reduced, inhibited,
suppressed, limited, controlled or prevented, etc.) Detecting an
alteration or an effect on HRF activity, function or expression can
be determined as set forth herein using in vitro assays or an
animal model.
[0160] Compounds useful in practicing the methods of the invention
include peptides and polypeptides, such as HRF sequences, HRF
subsequences or fragments (e.g., a sequence that binds to an Ig,
such as an IgE), antibodies and antibody subsequences (e.g.,
polyclonal or monoclonal and any of IgM, IgG, IgA, IgD or IgE
isotypes) known to the skilled artisan and as set forth herein.
Such sequences can be mammalian, humanized, human or chimeric.
[0161] Particular examples include a fragment of HRF/TCTP
polypeptide that binds to an immunoglobulin, such as an IgE. An
exemplary HRF sequence includes or consists of amino acids 1-19 or
amino acids 79-142 of a mammalian HRH/TCTP sequence, for example,
all or a portion of a MIIYRDLISHDEMFSDIYK (SEQ ID NO:1) sequence,
or all or a portion of a
QETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQ FFIGENMNP
(SEQ ID NO:2) sequence.
[0162] Particular non-limiting examples of HRF binding antibodies
include commercial antibodies from Santa Cruz Biotechnology, Inc.
(Santa Cruz, Calif.), as set forth in Table 2 below:
TABLE-US-00003 TABLE 2 HRF (B-3) Antibody sc-133131 mouse IgG.sub.1
1-172 (h) HRF (FL-172) Antibody sc-30124 rabbit IgG FL (h) HRF
(L-20) Antibody sc-20427 goat IgG internal (h) HRF (N-20) Antibody
sc-20426 goat IgG N-terminus (h) HRF (23-Y) Antibody sc-100763
mouse IgG.sub.1 FL (h) HRF (20) Antibody sc-135940 mouse IgG.sub.1
N/A
[0163] Additional particular non-limiting examples of HRF binding
antibodies include commercial antibodies from Assay
Designs/Stressgen (Ann Arbor, Mich.), Proteintech Group (Chicago,
Ill.), R and D Systems, Inc. (Minneapolis, Minn.), Sigma-Aldrich
Corp. (St. Louis, Mo.), AbDSerotec/MorphoSys UK Ltd. (Oxford, UK),
Strategic Diagnostics (SDIX) (Newark, Del.), Abcam (Cambridge,
Mass.) and Novus Biologicals, LLC (Littleton, Colo.) as set forth
in Table 3 below:
TABLE-US-00004 TABLE 3 Tumor protein (TPT1) Monoclonal Antibody
(3C7), Assay Designs/ Stressgen, reactivity: human; clonality:
monoclonal; host: mouse TPT1, Proteintech Group, reactivity: human;
clonality: polyclonal; host: rabbit Human/Mouse/Rat TPT1/TCTP MAb
(Clone 488411), R& D Systems, reactivity: human; clonality:
monoclonal; host: mouse Monoclonal Anti-TPT1 antibody produced in
mouse, Sigma-Aldrich, reactivity: human; clonality: monoclonal;
host: mouse Mouse anti-human TPT1: Azide Free, AbDSerotec,
reactivity: human; clonality: monoclonal; host: mouse TPT1
antibody, Strategic Diagnostics, reactivity: human; clonality:
polyclonal; host: rabbit TCTP antibody, Abcam, reactivity: human,
rat, mouse; clonality: polyclonal; host: rabbit TCTP antibody,
Abcam, reactivity: human; clonality: monoclonal; host: mouse TCTP
antibody, Abcam, reactivity: human; clonality: monoclonal; host:
mouse TCTP Antibody, Novus Biologicals, reactivity: human, rat;
clonality: polyclonal; host: rabbit TCTP Antibody (3C7), Novus
Biologicals, reactivity: human; clonality: monoclonal; host: mouse
TCTP Antibody (2C4), Novus Biologicals, reactivity: human;
clonality: monoclonal; host: mouse
[0164] The condition treated in accordance with the methods can be
chronic or acute. For example the food allergy, allergic reaction,
hypersensitivity, inflammatory response, inflammation, or airway
constriction can be chronic or acute.
[0165] Methods (e.g., treatment) according to the invention can
result in a reduction in occurrence, frequency, severity,
progression, or duration of a symptom of the condition. For
example, methods of the invention can protect against or decrease,
reduce, inhibit, suppress, limit or control progression, severity,
frequency, duration or probability of an adverse symptom of the
food allergy, allergic reaction, hypersensitivity, inflammatory
response or inflammation.
[0166] In particular embodiments, treatment according to a method
of the invention is sufficient to protect against or decrease,
reduce, inhibit, suppress, limit or control the food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation, decrease, reduce, inhibit, suppress, limit or control
susceptibility to the food allergy, allergic reaction or
hypersensitivity, or decrease, reduce, inhibit, suppress, limit or
control a food allergy, allergic reaction, hypersensitivity,
inflammatory response or inflammation.
[0167] In additional particular embodiments, treatment according to
a method of the invention is sufficient to decrease, reduce,
inhibit, suppress, limit, control or improve the probability,
severity, frequency, or duration of one or more adverse symptoms,
disorders, illnesses, pathologies, diseases, or complications
caused by or associated with the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation.
[0168] Exemplary symptoms include one or more of diarrhea, bloat,
swelling, pain, rash, headache, fever, nausea, lethargy, airway
constriction, skeletal joint stiffness, or tissue or cell damage.
Exemplary symptoms also include tissue, organ or cellular damage or
remodeling. Exemplary tissues and organs that can exhibit damage
include epidermal or mucosal tissue, gut, bowel, pancreas, thymus,
liver, kidney, spleen, skin, eye, or a skeletal joint (e.g., knee,
ankle, hip, shoulder, wrist, finger, toe, or elbow), and airway.
Treatment can result in decreasing, reducing, inhibiting,
suppressing, limiting, controlling or preventing progression or
worsening of tissue, organ or cellular damage or remodeling.
[0169] Methods of the invention that include treatment include
protecting against or decreasing, reducing, inhibiting,
suppressing, limiting or controlling occurrence, progression,
severity, frequency or duration of a symptom or characteristic of
the condition treated. At the whole body, regional or local level,
a symptom of an inflammatory response or inflammation is generally
characterized by swelling, pain, headache, fever, nausea, skeletal
joint stiffness or lack of mobility, rash, redness or other
discoloration. At the whole body level, an adverse symptom can also
include shortness of breath (dyspnea), wheezing, stridor, coughing,
airway remodeling, rapid breathing (tachypnea), prolonged
expiration, runny nose, rapid or increased heart rate
(tachycardia), rhonchous lung, lung or airway constriction,
over-inflation of the chest or chest-tightness, decreased lung
capacity, an acute asthmatic episode, lung, airway or respiratory
mucosum inflammation, or lung, airway or respiratory mucosum tissue
damage.
[0170] At the cellular level, a symptom of an inflammatory response
or inflammation is characterized by one or more of cell
infiltration of the region, production of antibodies, production of
cytokines, lymphokines, chemokines, interferons and interleukins,
cell growth and maturation factors (e.g., differentiation factors),
cell proliferation, cell differentiation, cell accumulation or
migration and cell, tissue or organ damage or remodeling. Thus,
treatment according to a method of the invention can protect
against or decrease, reduce, inhibit, suppress, limit or control
occurrence, progression, severity, frequency or duration of any one
or more of such symptoms or characteristics of the condition.
[0171] Allergic reactions in which treatment according to a method
of the invention can protect against or decrease, reduce, inhibit,
suppress, limit or control include bronchial asthma (extrinsic or
intrinsic); Allergic rhinitis; Onchocercal dermatitis; Atopic
dermatitis; allergic conjunctivitis; Drug reactions; Nodules,
eosinophilia, rheumatism, dermatitis, and swelling (NERDS);
Esophageal and a gastrointestinal allergy (e.g., a food
allergy).
[0172] Conditions in which treatment according to a method of the
invention can protect against or decrease, reduce, inhibit,
suppress, limit or control include hypersensitivity, inflammatory
response or inflammation of a respiratory disease or disorder. Such
disorders can affect the skin, or upper or lower respiratory tract,
and include, for example, asthma, allergic asthma, bronchiolitis
and pleuritis, as well as Airway Obstruction, Apnea, Asbestosis,
Atelectasis, Berylliosis, Bronchiectasis, Bronchiolitis,
Bronchiolitis Obliterans Organizing Pneumonia, Bronchitis,
Bronchopulmonary Dysplasia, Empyema, Pleural Empyema, Pleural
Epiglottitis, Hemoptysis, Hypertension, Kartagener Syndrome,
Meconium Aspiration, Pleural Effusion, Pleurisy, Pneumonia,
Pneumothorax, Respiratory Distress Syndrome, Respiratory
Hypersensitivity, Rhinoscleroma, Scimitar Syndrome, Severe Acute
Respiratory Syndrome, Silicosis, Tracheal Stenosis, eosinophilic
pleural effusions, Histiocytosis; chronic eosinophilic pneumonia;
hypersensitivity pneumonitis; Allergic bronchopulmonary
aspergillosis; Sarcoidosis; Idiopathic pulmonary fibrosis;
pulmonary edema; pulmonary embolism; pulmonary emphysema; Pulmonary
Hyperventilation; Pulmonary Alveolar Proteinosis; Chronic
Obstructive Pulmonary Disease (COPD); Interstitial Lung Disease;
and Topical eosinophilia.
[0173] In accordance with the invention, there are also provided
methods for increasing, enhancing or stimulating airway-dilation,
and for reducing or inhibiting airway-constriction. In one
embodiment, a method includes administering to a subject in need of
increasing airway-dilation an amount of a compound that inhibits or
reduces binding of HRF/TCTP to an immunoglobulin sufficient to
increase, enhance or stimulate airway-dilation in the subject. In
another embodiment, a method includes administering to a subject in
need thereof an amount of a compound that inhibits or reduces
binding of HRF/TCTP to an immunoglobulin sufficient to reduce or
inhibit airway-constriction in the subject.
[0174] In methods of the invention, a compound can be administered
prior to, substantially contemporaneously with or following one or
more adverse symptoms, disorders, illnesses, pathologies, diseases,
or complications caused by or associated with development of or
manifestation of an acute or chronic symptom, for example, a food
allergy, allergic reaction, hypersensitivity, inflammatory response
or inflammation. In methods of the invention, a compound can be
administered prior to, substantially contemporaneously with or
following administering a second drug or treatment.
[0175] Non-limiting examples of classes of second drugs or
treatments include an anti-food allergy, anti-allergic reaction,
anti-hypersensitivity, anti-inflammatory, anti-asthmatic or
anti-allergy drug. More particular examples of a second drug
include a hormone, a steroid, an anti-histamine, anti-leukotriene,
anti-IgE, anti-a4 integrin, anti-132 integrin, anti-CCR3
antagonist, .beta.2 agonist or an anti-selectin.
[0176] Methods of the invention may be practiced prior to (i.e.
prophylaxis), concurrently with or after evidence of the disorder,
disease or condition beginning (e.g., one or more symptoms).
Administering a composition prior to, concurrently with or
immediately following development of a symptom may decrease,
reduce, inhibit, suppress, limit or control the occurrence,
frequency, severity, progression, or duration of one or more
adverse symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with the food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation in the subject. In addition, administering a
composition prior to, concurrently with or immediately following
development of one or more symptoms may decrease, reduce, inhibit,
suppress, limit, control or prevent damage to cells, tissues or
organs that occurs, for example, due to one or more adverse
symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with a food allergy, allergic
reaction, hypersensitivity, inflammatory response or
inflammation.
[0177] Compositions and the methods of the invention, such as
treatment methods, can provide a detectable or measurable
therapeutic benefit or improvement to a subject. A therapeutic
benefit or improvement is any measurable or detectable, objective
or subjective, transient, temporary, or longer-term benefit to the
subject or improvement in the condition, disorder or disease, or
one or more adverse symptoms, disorders, illnesses, pathologies,
diseases, or complications caused by or associated with the food
allergy, allergic reaction, hypersensitivity, inflammatory response
or inflammation. Therapeutic benefits and improvements include, but
are not limited to, decreasing, reducing, inhibiting, suppressing,
limiting or controlling the occurrence, frequency, severity,
progression, or duration of one or more symptoms or complications
associated with disorders, illnesses, pathologies, diseases, or
complications caused by or associated with the food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation. Compositions and methods of the invention therefore
include providing a therapeutic benefit or improvement to a
subject.
[0178] In the methods of the invention in which a therapeutic
benefit or improvement is a desired outcome, a composition of the
invention such as an HRF polypeptide or an antibody that binds to
HRF, can be administered in a sufficient or effective amount to a
subject in need thereof. An "amount sufficient" or "amount
effective" refers to an amount that provides, in single or multiple
doses, alone or in combination, with one or more other compositions
(therapeutic agents such as a drug), treatments, protocols, or
therapeutic regimens agents, a detectable response of any duration
of time (long or short term), an expected desired outcome in or a
benefit to a subject of any measurable or detectable degree or for
any duration of time (e.g., for minutes, hours, days, months,
years, or cured). For example, a sufficient amount of an HRF
sequence, or an antibody or subsequence that binds to HRF, is
considered as having a therapeutic effect if administration results
in a decreased or reduced amount or frequency of immunotherapy
being required for treatment of a one or more adverse symptoms,
disorders, illnesses, pathologies, diseases, or complications
caused by or associated with the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation.
[0179] The doses or "sufficient amount" or "effective amount" for
treatment (e.g., to provide a therapeutic benefit or improvement)
typically are effective to ameliorate a disorder, disease or
condition, or one, multiple or all adverse symptoms, consequences
or complications of the disorder, disease or condition, one or more
adverse symptoms, disorders, illnesses, pathologies, diseases, or
complications, for example, caused by or associated with the food
allergy, allergic reaction, hypersensitivity, inflammatory response
or inflammation to a measurable extent, although decreasing,
reducing, inhibiting, suppressing, limiting or controlling a
progression or worsening of the disorder, disease or condition or a
symptom, is a satisfactory outcome.
[0180] The term "ameliorate" means a detectable improvement in a
subject's condition. A detectable improvement includes a subjective
or objective decrease, reduction, inhibition, suppression, limit or
control in the occurrence, frequency, severity, progression, or
duration of a symptom caused by or associated with a disorder,
disease or condition, such as one or more adverse symptoms,
disorders, illnesses, pathologies, diseases, or complications
caused by or associated with the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation, or an
improvement in an underlying cause or a consequence of the
disorder, disease or condition, or a reversal of the disorder,
disease or condition.
[0181] Treatment can therefore result in decreasing, reducing,
inhibiting, suppressing, limiting, controlling or preventing a
disorder, disease or condition, or an associated symptom or
consequence, or underlying cause; decreasing, reducing, inhibiting,
suppressing, limiting, controlling or preventing a progression or
worsening of a disorder, disease, condition, symptom or
consequence, or underlying cause; or further deterioration or
occurrence of one or more additional symptoms of the disorder,
disease condition, or symptom. Thus, a successful treatment outcome
leads to a "therapeutic effect," or "benefit" of decreasing,
reducing, inhibiting, suppressing, limiting, controlling or
preventing the occurrence, frequency, severity, progression, or
duration of one or more symptoms or underlying causes or
consequences of a condition, disorder, disease or symptom in the
subject, such as one or more adverse symptoms, disorders,
illnesses, pathologies, diseases, or complications caused by or
associated with a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation. Treatment
methods affecting one or more underlying causes of the condition,
disorder, disease or symptom are therefore considered to be
beneficial. Stabilizing a disorder or condition is also a
successful treatment outcome.
[0182] A therapeutic benefit or improvement therefore need not be
complete ablation of any one, most or all symptoms, complications,
consequences or underlying causes associated with the condition,
disorder or disease. Thus, a satisfactory endpoint is achieved when
there is an incremental improvement in a subject's condition, or a
partial decrease, reduction, inhibition, suppression, limit,
control or prevention in the occurrence, frequency, severity,
progression, or duration, or inhibition or reversal, of one or more
associated adverse symptoms or complications or consequences or
underlying causes, worsening or progression (e.g., stabilizing one
or more symptoms or complications of the condition, disorder or
disease), of one or more of the physiological, biochemical or
cellular manifestations or characteristics of the disorder or
disease, such as one or more adverse symptoms, disorders,
illnesses, pathologies, diseases, or complications caused by or
associated with the food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation, over a
short or long duration of time (hours, days, weeks, months,
etc.).
[0183] An amount sufficient or an amount effective can but need not
be provided in a single administration, may require multiple
administrations, and, can but need not be, administered alone or in
combination with another composition (e.g., agent), treatment,
protocol or therapeutic regimen. For example, the amount may be
proportionally increased as indicated by the need of the subject,
status of the disorder, disease or condition treated or the side
effects of treatment. In addition, an amount sufficient or an
amount effective need not be sufficient or effective if given in
single or multiple doses without a second composition (e.g.,
agent), treatment, protocol or therapeutic regimen, since
additional doses, amounts or duration above and beyond such doses,
or additional compositions (e.g., agents), treatments, protocols or
therapeutic regimens may be included in order to be considered
effective or sufficient in a given subject. Amounts considered
sufficient also include amounts that result in a reduction of the
use of another treatment, therapeutic regimen or protocol.
[0184] An amount sufficient or an amount effective need not be
effective in each and every subject treated, prophylactically or
therapeutically, or a majority of treated subjects in a given group
or population. An amount sufficient or an amount effective means
sufficiency or effectiveness in a particular subject, not a group
or the general population. As is typical for such methods, some
subjects will exhibit a greater response, or less or no response to
a treatment method.
[0185] Additional examples of a therapeutic benefit for one or more
adverse symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with the food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation is an improvement in one or more such symptoms. For
example, decreasing, reducing, inhibiting, suppressing, limiting,
controlling or preventing further or reducing an allergic reaction,
food allergy, lung or airway constriction or remodeling, tissue or
organ infiltration or tissue destruction, or pancreas, thymus,
kidney, liver, spleen, eye, epidermal (skin) or mucosal tissue, gut
or bowel infiltration or tissue destruction or remodeling.
[0186] Particular non-limiting examples of therapeutic benefit or
improvement for a pathogen include decreasing, reducing,
inhibiting, suppressing, limiting, controlling or preventing
occurrence, frequency, severity, progression, or duration of one or
more symptoms or complications of a food allergy, allergic
reaction, hypersensitivity, inflammatory response or inflammation.
Additional particular non-limiting examples of therapeutic benefit
or improvement include stabilizing the condition (i.e., decreasing,
reducing, inhibiting, suppressing, limiting, controlling or
preventing a worsening or progression of a symptom or complication
associated with a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation). Symptoms
or complications associated with a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation whose
occurrence, frequency, severity, progression, or duration can be
decreased, reduced, inhibited, suppressed, limited, controlled or
prevented are known in the art. A therapeutic benefit can also
include reducing susceptibility of a subject to one or more adverse
symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with a food allergy, allergic
reaction, hypersensitivity, inflammatory response or inflammation
or hastening or accelerating recovery from one or more adverse
symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with a food allergy, allergic
reaction, hypersensitivity, inflammatory response or
inflammation.
[0187] As is typical for treatment or therapeutic methods, some
subjects will exhibit greater or less response to a given
treatment, therapeutic regiment or protocol. Thus, appropriate
amounts will depend upon the condition treated (e.g., the type or
stage of the tumor), the therapeutic effect desired, as well as the
individual subject (e.g., the bioavailability within the subject,
gender, age, etc.).
[0188] The term "subject" refers to animals, typically mammalian
animals, such as humans, non human primates (apes, gibbons,
chimpanzees, orangutans, macaques), domestic animals (dogs and
cats), farm animals (horses, cows, goats, sheep, pigs) and
experimental animal (mouse, rat, rabbit, guinea pig). Subjects
include animal disease models, for example, animal models of food
allergy, allergic reaction, hypersensitivity, inflammatory response
or inflammation, for studying in vivo a composition of the
invention, for example, an HRF sequence or an antibody that binds
to HRF.
[0189] Subjects appropriate for treatment include those having or
at risk of having an undesirable or aberrant immune response,
immune disorder or immune disease, those undergoing treatment for
an undesirable or aberrant immune response, immune disorder or
immune disease as well as those who are undergoing or have
undergone treatment or therapy for an undesirable or aberrant
immune response, immune disorder or immune disease, including
subjects where the undesirable or aberrant immune response, immune
disorder or immune disease is in remission. Specific non-limiting
examples include subjects having or at risk of having one or more
adverse symptoms, disorders, illnesses, pathologies, diseases, or
complications caused by or associated with a food allergy, allergic
reaction, hypersensitivity, inflammatory response or
inflammation.
[0190] "At risk" subjects typically have risk factors associated
with undesirable or aberrant immune response, immune disorder or
immune disease, such as a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation. Risk
factors include gender, lifestyle (diet, smoking), occupation
(medical and clinical personnel, agricultural and livestock
workers), environmental factors (allergen exposure), family history
(e.g., genetic predisposition), etc.
[0191] Compositions and methods of the invention may be contacted
or provided in vitro, ex vivo or administered in vivo. Compositions
can be administered to provide the intended effect as a single or
multiple dosages, for example, in an effective or sufficient
amount. Exemplary doses range from about 25-250, 250-500, 500-1000,
1000-2500 or 2500-5000, 5000-25,000, 5000-50,000 pg/kg; from about
50-500, 500-5000, 5000-25,000 or 25,000-50,000 ng/kg; and from
about 25-250, 250-500, 500-1000, 1000-2500 or 2500-5000,
5000-25,000, 5000-50,000 mg/kg, on consecutive days, alternating
days or intermittently.
[0192] Single or multiple doses can be administered on the same or
consecutive days, alternating days or intermittently. For example,
a compound such as an HRF sequence or antibody that binds to HRF
can be administered one, two, three, four or more times daily, on
alternating days, bi-weekly, weekly, monthly, bi-monthly, or
annually.
[0193] Compounds can be administered to a subject and methods may
be practiced substantially contemporaneously with, or within about
1-60 minutes, hours, or days of the onset of an adverse symptom
associated with a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation.
[0194] Compounds can be administered and methods may be practiced
via systemic, regional or local administration, by any route. For
example, an HRF sequence or an antibody that binds to HRF may be
administered systemically, regionally or locally, via ingestion,
via inhalation, topically, intravenously, orally (e.g., ingestion
or inhalation), intramuscularly, intraperitoneally, intradermally,
subcutaneously, intracavity, intracranially, transdermally
(topical), parenterally, e.g. transmucosally or rectally.
Compositions and methods of the invention including pharmaceutical
formulations can be administered via a (micro)encapsulated delivery
system or packaged into an implant for administration.
[0195] Invention compositions and methods include pharmaceutical
compositions, which refer to "pharmaceutically acceptable" and
"physiologically acceptable" carriers, diluents or excipients. As
used herein, the term "pharmaceutically acceptable" and
"physiologically acceptable," when referring to carriers, diluents
or excipients includes solvents (aqueous or non-aqueous),
detergents, solutions, emulsions, dispersion media, coatings,
isotonic and absorption promoting or delaying agents, compatible
with pharmaceutical administration and with the other components of
the formulation. Such formulations can be contained in a tablet
(coated or uncoated), capsule (hard or soft), microbead, emulsion,
powder, granule, crystal, suspension, syrup or elixir.
[0196] Pharmaceutical compositions can be formulated to be
compatible with a particular route of administration. Compositions
for parenteral, intradermal, or subcutaneous administration can
include a sterile diluent, such as water, saline solution, fixed
oils, polyethylene glycols, glycerine, propylene glycol or other
synthetic solvents. The preparation may contain one or more
preservatives to prevent microorganism growth (e.g., antibacterial
agents such as benzyl alcohol or methyl parabens; antioxidants such
as ascorbic acid or sodium bisulfite; chelating agents such as
ethylenediaminetetraacetic acid; buffers such as acetates, citrates
or phosphates and agents for the adjustment of tonicity such as
sodium chloride or dextrose).
[0197] Pharmaceutical compositions for injection include sterile
aqueous solutions (where water soluble) or dispersions and sterile
powders for the extemporaneous preparation of sterile injectable
solutions or dispersion. For intravenous administration, suitable
carriers include physiological saline, bacteriostatic water,
Cremophor EL.TM. (BASF, Parsippany, N.J.) or phosphate buffered
saline (PBS). The carrier can be a solvent or dispersion medium
containing, for example, water, ethanol, polyol (e.g., glycerol,
propylene glycol, and polyetheylene glycol), and suitable mixtures
thereof. Fluidity can be maintained, for example, by the use of a
coating such as lecithin, or by the use of surfactants.
Antibacterial and antifungal agents include, for example, parabens,
chlorobutanol, phenol, ascorbic acid and thimerosal. Including an
agent that delays absorption, for example, aluminum monostearate
and gelatin, can prolong absorption of injectable compositions.
[0198] For transmucosal or transdermal administration, penetrants
appropriate to the barrier to be permeated are used in the
formulation. Such penetrants are known in the art, and include, for
example, for transmucosal administration, detergents, bile salts,
and fusidic acid derivatives. Transmucosal administration can be
accomplished through the use of nasal sprays, inhalation devices
(e.g., aspirators) or suppositories. For transdermal
administration, the active compounds are formulated into ointments,
salves, gels, creams or patches.
[0199] Additional pharmaceutical formulations and delivery systems
are known in the art and are applicable in the methods of the
invention (see, e.g., Remington's Pharmaceutical Sciences (1990)
18th ed., Mack Publishing Co., Easton, Pa.; The Merck Index (1996)
12th ed., Merck Publishing Group, Whitehouse, N.J.; Pharmaceutical
Principles of Solid Dosage Forms, Technonic Publishing Co., Inc.,
Lancaster, Pa., (1993); and Poznansky, et al., Drug Delivery
Systems, R. L. Juliano, ed., Oxford, N.Y. (1980), pp. 253-315).
[0200] The compositions used in accordance with the invention,
including proteins (HRF sequences, antibodies), nucleic acid (e.g.,
inhibitory), treatments, therapies, agents, drugs and
pharmaceutical formulations can be packaged in dosage unit form for
ease of administration and uniformity of dosage. "Dosage unit form"
as used herein refers to physically discrete units suited as
unitary dosages treatment; each unit contains a quantity of the
composition in association with the carrier, excipient, diluent, or
vehicle calculated to produce the desired treatment or therapeutic
(e.g., beneficial) effect. The unit dosage forms will depend on a
variety of factors including, but not necessarily limited to, the
particular composition employed, the effect to be achieved, and the
pharmacodynamics and pharmacogenomics of the subject to be
treated.
[0201] The invention provides cell-free (e.g., in solution, in
solid phase) and cell-based (e.g., in vitro or in vivo) methods of
screening, detecting and identifying HRF. The methods can be
performed in solution, in vitro using a biological material or
sample, and in vivo, for example, using a fluid or lavage sample
from an animal.
[0202] In accordance with the invention, there are provided methods
of diagnosing a subject having or at risk of a food allergy. In one
embodiment, a method includes measuring histamine releasing factor
(HRF)/translationally controlled tumor protein (TCTP) in a sample
from a subject, wherein an amount of HRF/TCTP in the sample greater
than normal diagnoses the subject as having or at risk of a food
allergy.
[0203] In one aspect, HRF measuring includes determining the amount
of HRF/TCTP protein or nucleic acid encoding HRF/TCTP(RNA, cDNA) in
the sample. In another aspect, HRF measuring includes contacting
the sample with an agent or tag (e.g., a detectable agent or tag,
such as an antibody, protein or nucleic acid that binds to HRF/TCTP
protein or nucleic acid encoding HRF/TCTP) that binds to HRF/TCTP
protein or nucleic acid encoding HRF/TCTP and ascertaining the
amount of HRF/TCTP protein or nucleic acid encoding HRF/TCTP, or
the amount of agent or tag (e.g., a detectable agent or tag, such
as an antibody, protein or nucleic acid that binds to HRF/TCTP
protein or nucleic acid encoding HRF/TCTP) bound to the HRF/TCTP
protein or nucleic acid encoding HRF/TCTP.
[0204] The invention also provides cell-free (e.g., in solution, in
solid phase) and cell-based (e.g., in vitro or in vivo) methods of
diagnosing and monitoring progression of a subject having or at
increased risk of having a food allergy, allergic reaction,
hypersensitivity, inflammatory response, or inflammation, the
location, presence or extent of a food allergy, allergic reaction,
hypersensitivity, inflammatory response, or inflammation, as well
as identifying a subject appropriated for treatment with an HRF
sequence, or an antibody that binds to HRF, due to increased
probability of responding to treatment. The methods can be
performed in solution, in vitro using a biological material or
sample, for example, a sample or biopsy of cells, tissue or organ.
The methods can also be performed in vivo, for example, in an
animal.
[0205] In one embodiment, a method includes contacting a biological
material or sample (e.g., from a subject) with an HRF sequence, or
an antibody that binds to HRF; and assaying for the presence of
HRF. The binding to HRF can be used to ascertain the presence or
amount of HRF, which can be correlated with increased risk of
having a food allergy, allergic reaction, hypersensitivity,
inflammatory response, or inflammation, thereby diagnosing the
subject. The presence or amount of HRF can also identify a subject
appropriate for an anti-HRF treatment, as such subjects will have a
greater probability of favorably responding to treatment of a food
allergy, allergic reaction, hypersensitivity, inflammatory
response, or inflammation, for example, treatment with an HRF
sequence (HRF polypeptide or inhibitory nucleic acid) or an
anti-HRF antibody. In one aspect, a biological material or sample
is obtained from a mammal (e.g., a human). Methods of monitoring
progression of a food allergy, allergic reaction, hypersensitivity,
inflammatory response, or inflammation can be performed at a
regular or irregular intervals, for example, daily, bi-weekly,
weekly, bi-monthly, monthly, quaterly, semi- or bi-annually,
annually, etc., as appropriate.
[0206] Diagnostic methods can be performed on any subject, such as
a mammal (e.g., human, primate). Such subjects can have or be at
risk of having a condition or disorder associated with HRF
activity, function, or expression as set forth herein. For example,
a subject can have or be at risk of having a food allergy, allergic
reaction, hypersensitivity, inflammatory response or
inflammation.
[0207] The terms "assaying" and "measuring" and grammatical
variations thereof are used interchangeably herein and refer to
either qualitative or quantitative determinations, or both
qualitative and quantitative determinations. When the terms are
used in reference to binding or detection, any means of assessing
the relative amount, affinity or specificity of binding is
contemplated, including the various methods set forth herein and
known in the art. For example, HRF binding can be assayed or
measured by an ELISA assay, Western blot or immunoprecipitation
assay, or by modulating an activity, function or expression of a
native HRF. In another example, antibody binding can be assayed or
measured by an ELISA assay, Western blot or immunoprecipitation
assay.
[0208] The term "correlating" and grammatical variations thereof
refers to a relationship or link between two or more entities. For
example, as disclosed herein HRF is associated with, among other
things, food allergy, allergic reaction, hypersensitivity,
inflammatory response or inflammation. Thus, because of this
relationship between HRF and a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation they
correlate with each other. Thus, correlating the presence or
quantity of HRF can indicate susceptibility, or the presence and/or
extent, or severity of a food allergy, allergic reaction,
hypersensitivity, inflammatory response or inflammation in a
subject, for example.
[0209] In accordance with the invention, there are provided methods
of identifying an agent that reduces or inhibits a food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation. In one embodiment, a method includes contacting
histamine releasing factor (HRF)/translationally controlled tumor
protein (TCTP) with a test compound in the presence of an
immunoglobulin that binds to HRF/TCTP; and determining if the
compound inhibits or reduces binding of HRF/TCTP to the
immunoglobulin. A reduction or inhibition of binding identifies the
test compound as an agent that reduces or inhibits a food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation. In particular aspects, an immunoglobulin is an IgE,
IgG, IgA, IgM or IgD.
[0210] The invention provides kits including compositions of the
invention (e.g., HRF polypeptides, antibodies that bind to HRF,
nucleic acids encoding HRF sequences or hybridizing sequences,
etc.), combination compositions and pharmaceutical formulations
thereof, packaged into suitable packaging material. Kits can be
used in various methods. For example, a kit can determine an
anti-N19 antibody or an anti-HRF antibody that recognizes epitopes
outside the N19 portion, since anti-N19 antibody is believed to
inhibit HRF/Ig interactions, but the latter anti-HRF antibody might
not have the same activity. If so, the ratio of anti-N-19 over
anti-HRF (outside of N19) in blood or other body fluids would
indicate a contribution of HRF/Ig interaction to the disease.
[0211] A kit typically includes a label or packaging insert
including a description of the components or instructions for use
in vitro, in vivo, or ex vivo, of the components therein. A kit can
contain a collection of such components, e.g., HRF sequence,
antibody that binds to HRF, alone, or in combination with another
therapeutically useful composition (e.g., an immune modulatory
drug).
[0212] The term "packaging material" refers to a physical structure
housing the components of the kit. The packaging material can
maintain the components sterilely, and can be made of material
commonly used for such purposes (e.g., paper, corrugated fiber,
glass, plastic, foil, ampules, vials, tubes, etc.).
[0213] Kits of the invention can include labels or inserts. Labels
or inserts include "printed matter," e.g., paper or cardboard, or
separate or affixed to a component, a kit or packing material
(e.g., a box), or attached to an ampule, tube or vial containing a
kit component. Labels or inserts can additionally include a
computer readable medium, such as a disk (e.g., hard disk), optical
disk such as CD- or DVD-ROM/RAM, DVD, MP3, magnetic tape, or an
electrical storage media such as RAM and ROM or hybrids of these
such as magnetic/optical storage media, FLASH media or memory type
cards.
[0214] Labels or inserts can include identifying information of one
or more components therein, dose amounts, clinical pharmacology of
the active ingredient(s) including mechanism of action,
pharmacokinetics and pharmacodynamics. Labels or inserts can
include information identifying manufacturer information, lot
numbers, manufacturer location and date.
[0215] Labels or inserts can include information on a condition,
disorder, disease or symptom for which a kit component may be used.
Labels or inserts can include instructions for the clinician or for
a subject for using one or more of the kit components in a method,
treatment protocol or therapeutic regimen. Instructions can include
dosage amounts, frequency or duration, and instructions for
practicing any of the methods, treatment protocols or therapeutic
regimes set forth herein. Exemplary instructions include,
instructions for treating an undesirable or aberrant immune
response, immune disorder, immune disease, such as a food allergy,
allergic reaction, hypersensitivity, inflammatory response or
inflammation. Kits of the invention therefore can additionally
include labels or instructions for practicing any of the methods of
the invention described herein including treatment, or diagnostic
methods.
[0216] Labels or inserts can include information on any benefit
that a component may provide, such as a prophylactic or therapeutic
benefit. Labels or inserts can include information on potential
adverse side effects, such as warnings to the subject or clinician
regarding situations where it would not be appropriate to use a
particular composition. Adverse side effects could also occur when
the subject has, will be or is currently taking one or more other
medications that may be incompatible with the composition, or the
subject has, will be or is currently undergoing another treatment
protocol or therapeutic regimen which would be incompatible with
the composition and, therefore, instructions could include
information regarding such incompatibilities.
[0217] Invention kits can additionally include other components.
Each component of the kit can be enclosed within an individual
container and all of the various containers can be within a single
package. Invention kits can be designed for cold storage. Invention
kits can further be designed to contain host cells expressing
peptides or antibodies of the invention, or that contain encoding
nucleic acids. The cells in the kit can be maintained under
appropriate storage conditions until the cells are ready to be
used. For example, a kit including one or more cells can contain
appropriate cell storage medium so that the cells can be thawed and
grown.
[0218] Unless otherwise defined, all technical and scientific terms
used herein have the same meaning as commonly understood by one of
ordinary skill in the art to which this invention belongs. Although
methods and materials similar or equivalent to those described
herein can be used in the practice or testing of the present
invention, suitable methods and materials are described herein.
[0219] All applications, publications, patents and other
references, GenBank citations and ATCC citations cited herein are
incorporated by reference in their entirety. In case of conflict,
the specification, including definitions, will control.
[0220] As used herein, the singular forms "a", "and," and "the"
include plural referents unless the context clearly indicates
otherwise. Thus, for example, reference to "an HRF sequence" or an
"HRF binding antibody" includes a plurality of such HRF sequences
or antibodies or subsequences thereof, and reference to "an HRF
activity or function" can include reference to one or more HRF
activities or functions, and so forth.
[0221] As used herein, numerical values are often presented in a
range format throughout this document. The use of a range format is
merely for convenience and brevity and should not be construed as
an inflexible limitation on the scope of the invention.
Accordingly, the use of a range expressly includes all possible
subranges, all individual numerical values within that range, and
all numerical values or numerical ranges include integers within
such ranges and fractions of the values or the integers within
ranges unless the context clearly indicates otherwise. This
construction applies regardless of the breadth of the range and in
all contexts throughout this patent document. Thus, for example,
reference to a range of 90-100% includes 91-99%, 92-98%, 93-95%,
91-98%, 91-97%, 91-96%, 91-95%, 91-94%, 91-93%, and so forth.
Reference to a range of 90-100%, includes 91%, 92%, 93%, 94%, 95%,
95%, 97%, etc., as well as 91.1%, 91.2%, 91.3%, 91.4%, 91.5%, etc.,
92.1%, 92.2%, 92.3%, 92.4%, 92.5%, etc., and so forth.
[0222] In addition, reference to a range of 1-5,000 fold includes
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,
20, fold, etc., as well as 1.1, 1.2, 1.3, 1.4, 1.5, fold, etc.,
2.1, 2.2, 2.3, 2.4, 2.5, fold, etc., and any numerical range within
such a ranges, such as 1-2, 5-10, 10-50, 50-100, 100-500, 100-1000,
500-1000, 1000-2000, 1000-5000, etc. In a further example,
reference to a range of K.sub.D 10.sup.-5 M to about K.sub.D
10.sup.-13 M includes any numerical value or range within or
encompassing such values, such as 1.times.10.sup.-5 M,
1.times.M10.sup.-6 M, 1.times.10.sup.-7 M, 1.times.10.sup.-8 M,
etc.
[0223] As also used herein a series of range formats are used
throughout this document. The use of a series of ranges includes
combinations of the upper and lower ranges to provide a range. This
construction applies regardless of the breadth of the range and in
all contexts throughout this patent document. Thus, for example,
reference to a series of ranges such as 5-10, 10-20, 20-30, 30-40,
40-50, 50-75, 75-100, 100-150, and 150-171, includes ranges such as
5-20, 5-30, 5-40, 5-50, 5-75, 5-100, 5-150, 5-171, and 10-30,
10-40, 10-50, 10-75, 10-100, 10-150, 10-171, and 20-40, 20-50,
20-75, 20-100, 20-150, 20-171, and so forth.
[0224] The invention is generally disclosed herein using
affirmative language to describe the numerous embodiments. The
invention also specifically includes embodiments in which
particular subject matter is excluded, in full or in part, such as
substances or materials, method steps and conditions, protocols,
procedures, assays or analysis. Thus, even though the invention is
generally not expressed herein in terms of what the invention does
not include, aspects that are not expressly included in the
invention are nevertheless disclosed herein.
[0225] A number of embodiments of the invention have been
described. Nevertheless, it will be understood that various
modifications may be made without departing from the spirit and
scope of the invention. Accordingly, the following examples are
intended to illustrate but not limit the scope of invention
described in the claims.
EXAMPLES
Example 1
[0226] This example includes a description of various materials and
methods.
Mice
[0227] C57BL/6 and Balb/c mice were purchased from the Jackson
Laboratory (Maine). Fc.epsilon.RI.alpha..sup.-/- mice (Dombrowicz
et al., Cell 75:969 (1993)) on a Balb/c background,
Fc.epsilon.RI.gamma..sup.-/- mice (Takai et al., Cell 76:519
(1994)) on a C57BL/6 background, .mu.MT mice (Kitamura et al.,
Nature 350:6317 (1991)) on a C57BL/6 background, and
Kit.sup.W-sh/W-sh (Grimbaldeston et al., Am. J. Pathol. 167:835
(2005)) on a C57BL/6 background. Such knockout mice are usually
backcrossed to Balb/c or C57BL/6 mice for >10 generations.
Purification and Preparation of IgE and IgG
[0228] For purification of IgE, Ishizaka's method (Ishizaka,
Methods Enzymol. 116:76 (1985)) was utilized such that IgE was
enriched from ascites or culture supernatants by ammonium sulfate
precipitation (40-55%). IgE-containing fractions were then purified
by DEAE-agarose (GE Healthcare Bio-Sciences AB) column
chromatography, followed by gel filtration using Sephacryl S-300
(GE). IgG molecules were first precipitated by 50% ammonium sulfate
solution and then purified by protein G column (GE) chromatography,
followed by gel filtration using Sephacryl S-300. The IgE and IgG
preparations were dialyzed against PBS and ultracentrifuged to
remove aggregates before use.
[0229] Anti-DNP mouse IgE mAbs (DNP H1-.epsilon.-26, DNP
H1-.epsilon.-206) were provided by Dr. Fu-Tong Liu, University of
California, Davis; anti-OVA mouse IgE (aOE) by Dr. Erwin W.
Gelfand, National Jewish Medical and Research Center. IgE mAbs or
anti-OVA mouse IgE are also available from Condrex, Inc., Redmond,
Wash. Anti-TNP mouse IgE mAbs (C48-2 and C38-2) and anti-dansyl
mouse IgE mAb (27-74) were purchased from BD Biosciences
Pharmingen. Anti-DNP IgE mAb (SPE-7) was purchased from
Sigma-Aldrich. IgG mAbs were provided by Stephen P. Shoenberger,
LIAI, Rachel S. Soloff, Lilia Koriazova, and Manju Saxena, Kyowa
Hakko Kirin California, or purchased from BD Pharmingen and
eBiosciences. Human IgE molecules from a myeloma patient (IgEK and
IgE X) were purchased from The Binding Site Inc. Human monoclonal
IgE (HE1) was purchased from Diatec Monoclonals AS. Human IgE mAb
(SKO-007) was purified from culture supernatants of hybridoma
(American Type Culture Collection).
Preparation of Recombinant Mouse Histamine Releasing Factor
(mHRF)
[0230] Complementary DNAs of full-length and truncated forms of
mHRF were amplified by PCR using total RNA derived from BMMCs
(mouse bone marrow-derived mast cells) as a template and the
primers. After nucleotide sequences of the PCR products were
confirmed, they were ligated into the pGEX-3T vector. GST-mHRF and
truncated fusion proteins were expressed in E. coli DH5.alpha. and
purified using glutathione-agarose (Signa-Aldrich). The
C-terminally His.sub.6-tagged mHRF construct was generated by
amplifying the cDNA of the full-length mHRF using the primers. The
amplified cDNA was ligated into pET-24a(+) plasmid (Novagen) and
mHRF-His.sub.6 was expressed in E. coli BL21(DE3) (Invitrogen). The
mHRF-His.sub.6 protein was purified using ProBond.TM.
Nickel-Chelating Resin (Invitrogen). All recombinants were further
purified by Sephacryl S-100 (GE) and extensively dialyzed against
PBS.
[0231] Primers used were as follows:
TABLE-US-00005 Forward primers for GST fusion proteins (SEQ ID NOs:
11-12) For N12, N19, N32, N47, N78, N142, N154 and full length
mouse HRF: AAAAGGATCCATGATCATCTACCGGGACC For N79-142 and N79-172:
AAAAGGATCCCAAGAAACCAGCTTCACAAA Reverse primers for GST fusion
proteins (SEQ ID NOs: 13-21) For N12: TTTGAATTCTTACTCGTCATGGCTGAT
N19: AAAGAATTCTTACTTGTAGATGTCGGAGAACA N32:
AAAGAATTCTTACTCCACCTCCAGGCACAGCC N47:
AAAGAATTCTTAGAGCGAGTCATCGATGGCAC N78:
AAAGAATTCTTATAAGTGATGGTTCATGACAA N142 and N79-142:
AAAGAATTCTTATGGATTCATGTTTTCACCAA Full length and N79-172:
AAAAGGATCCCAAGAAACCAGCTTCACAAA Forward primer for His6-tagged mouse
HRF: AAAACATATGATCATCTACCGGGACCT Reverse primer for His6-tagged
mouse HRF: TTTTCTCGAGACATTTCTCCATCTCTAAGC
ELISA
[0232] For detection of HRF-reactive mouse IgE and IgG, 96-well
ELISA plates (PRO-BIND.TM. Flat Bottom from BD Labware) were coated
with GST, GST-mHRF or mHRF-His.sub.6 (each at 10 .mu.g/ml in 0.1 M
carbonate buffer (pH 9.5)). The next day, the plates were washed
and blocked with 10% FCS or 1% BSA. Then, mouse IgE and IgG
molecules (10 .mu.g/ml), plasma (1/100 dilution) and BAL fluids
(1/10 dilution) were incubated in the coated wells. Bound IgE was
detected by incubation with biotinylated anti-mouse IgE
(Pharmingen), followed with HRP-conjugated streptavidin (BD
Pharmingen). Bound IgG was detected by incubation with
HRP-conjugated anti-mouse IgG (GE). Color was developed using TMB
substrate (BD Biosciences) and absorbance at OD.sub.450 was
measured. For detection of HRF-reactive human IgE molecules,
biotinylated anti-human IgE mAb (Pharmingen) was used in place of
biotinylated anti-mouse IgE mAb.
[0233] For OVA-specific IgG detection, OVA (10 .mu.g/ml;
Sigma-Aldrich) was coated onto 96-well ELISA plates overnight. BAL
fluids (1/100 dilution) and plasma (1/1000 dilution) were
incubated, and bound IgG molecules were detected by incubation with
HRP-conjugated anti-mouse IgG. The absorbance at 450 nm was
measured after color was developed.
[0234] IL-5 and IL-13 were measured by ELISA kits purchased from BD
Biosciences and R&D Systems, respectively.
Mast Cells
[0235] Bone marrow cells from mouse femurs were cultured in
IL-3-containing medium for 4 to 6 weeks to generate bone marrow
mast cells (BMMCs). Purity of mast cells (>95%) was checked by
flow cytometry (c-Kit.sup.+ Fc.epsilon.RI.sup.+). For purification
of peritoneal mast cells, peritoneal cells were first collected by
lavage with 1 ml PBS. Then c-Kit.sup.+ cells were purified by
labeling with PE-conjugated anti-mouse CD117 mAb (StemCell
Technologies Inc), followed by incubation with magnetic
bead-conjugated anti-PE mAb (StemCell). The cells were separated
using EasySep.RTM. magnet (StemCell).
GST Pull-Down Assay
[0236] IgE molecules were incubated with either GST or GST-mHRF
bound onto glutathione-agarose beads overnight at 4.degree. C. The
beads were washed with PBS in the presence of 1% Triton-X and
resuspended in SDS sample buffer, then, boiled. Samples were
analyzed by SDS-PAGE, followed by immunoblotting using anti-GST
(Santa Cruz Biotechnology) and anti-mouse IgE (Southern
Biotechnology Associates, Inc.).
Flow Cytometric Analysis
[0237] For detection of the interaction between HRF and IgE
molecules, BMMCs were first sensitized with 50 .mu.g/ml IgE at
4.degree. C. for 20 minutes, and then were incubated with
mHRF-His.sub.6 at 4.degree. C. for 2 hours. Cell-bound mHRF was
detected by incubation with rabbit anti-His Ab (Santa Cruz
Biotechnology), followed with Alexa647-conjugated anti-rabbit IgG
(Molecular Probe). FACSCalibur (BD Biosciences) was used for
analysis.
Quartz Crystal Microbalance (QCM)
[0238] A QCM-based assay was performed as described previously
(Ozeki et al., Anal. Chem. 79:79 (2007)). GST-mHRF or
mHRF-His.sub.6 was immobilized onto the chip and the immobilized
chip was blocked with 1% BSA-PBS. Then, IgG molecules were injected
and measured using Affinix Q.sup.4 apparatus (Initium Co.
Ltd.).
Degranulation
[0239] Mast cells were sensitized with 0.5 .mu.g/ml IgE overnight.
The mast cells were stimulated with TNP.sub.26-BSA or
mHRF-His.sub.6 for 40 minutes in Tyrode's buffer. The amount of
.beta.-hexosaminidase (.beta.-hex) in both supernatants and cell
pellets was measured. The percentage of .beta.-hex release was
calculated as follows: % release=.beta.-hex in
supernatants/(.beta.-hex in supernatants+.beta.-hex in cell
pellets)
Passive Cutaneous Anaphylaxis
[0240] Mouse ears were first sensitized with 0.5 .mu.g
anti-TNP(C38-2 in the right ear and C48-2 in the left ear) IgE.
After 24 hours, Evans' blue dye was intravenously (i.v.) injected,
and TNP.sub.26-BSA (Ag: 0.1 .mu.g) or mHRF-His.sub.6 (10 .mu.g)
were immediately injected into the ears. The acute (30 minute)
reactions were analyzed by measurement of Evans' blue dye leakage.
The ear thickness was measured using a micrometer (Mitutoyo) at
0.5, 1, 2, 4, 6, 8, 12, and 24 hours after mHRF-His.sub.6
challenge. As a negative control, saline was injected into the ears
in place of antigen or HRF challenge. IgG-mediated response was
performed by sensitization with 2 .mu.g IgG1 (in place of anti-TNP
IgE) and challenge with anti-mouse IgG1 Ab (0.6 .mu.g: Southern
Biotechnology, in place of TNP.sub.26-BSA). Engraftmanet of
Kit.sup.W-sh/W-sh mice with BMMCs was performed 6 weeks before
study (Nakano, T., et al. J Exp Med 162:1025-1043 (1985)). In some
studies, mice were sensitized and stimulated as described above but
without Evans blue.
Airway Inflammation
[0241] Mice were sensitized with OVA (10 .mu.g in 0.1 ml saline) at
days 0, 7, 14, 21, 28, and 35. Sensitized mice were challenged with
OVA (20 .mu.g in 20 .mu.l saline) at days 40, 43 and 46. Some mice
were pretreated with GST, GST-N19 (400 .mu.g in PBS), or N19
peptide (40 .mu.g in 2% DMSO-containing PBS) 15 minutes before OVA
challenge. Twenty-four hours after the last challenge, lung
function in response to methacholine was tested using FlexiVent
system (SCIREQ Scientific Respiratory Equiptment). Mice were
sacrificed and plasma, BAL fluids, and lung tissues were collected.
Cells in BAL fluids were counted, and specific cell numbers were
calculated after May-Giemsa staining of Cytospin preparations
Immunofluorescence microscopy was performed on
membrane-permeabilized and non-permeabilized lung tissues. For
staining of lung tissues by staining of H&E and periodic
acid-Schiff (PAS), lung tissues were fixed with formalin and
embedded in paraffin. For measurement of cytokine levels in lung
tissues, lung homogenates were prepared (Oyoshi et al., Immunol.
120:303 (2007)) and cytokines quantified by ELISA. HRF in lung
homogenates and plasma was SDS-PAGE and immunoblotting with
anti-HRF Ab (Santa Cruz Biotechnology).
[0242] In the second model (Mathias et al., J Immunol. 182:2416
(2009)), BALB/c mice were intranasally treated with Aspergillus
fumigatus allergen (50 ul, Greer Laboratories) or PBS three times
per week for 3 weeks. Some moce were intranasally pretreated with
GST or GST-N19 (200 ug/50 ul) from the second week 30 min before
each immunization. Twenty four hours after the last challenge, mice
were sacrificed and BAL fluids, blood and lung tissues were
collected.
Immunofluorescence Microscopy
[0243] For detection of intracellular and extracellular HRF, lung
tissues were fixed with 4% paraformaldehyde and embedded in O.C.T.
compound (Sakura Finetek U.S.A.). For staining of extracellular
HRF, fixed lung sections were incubated with anti-HRF Ab overnight.
Washed sections were incubated with Alexa647-conjugated anti-rabbit
IgG for 1 hour, then with Alexa555-conjugated wheat germ agglutinin
(WGA) for 10 minutes. The specimens were mounted with ProLong.RTM.
Gold antifade reagent with DAPI (Invitrogen). For staining of
intracellular HRF, the sections were stained with
Alexa555-conjugatted WGA for 10 minutes and treated with cold
methanol for permeabilization. Then, the sections were incubated
with anti-HRF Ab overnight, incubated with Alexa647-conjugated
anti-rabbit IgG Ab for 1 hour, and mounted with ProLong.RTM. Gold
antigade reagent with DAPI.
Histology
[0244] Lung tissues were fixed with 10% formaldehyde and embedded
in paraffin. Six-micrometer sections were stained with H&E. For
immunofluorescence, biopsies were fixed in 4% paraformadehyde at
4.degree. C., washed in 10-20% sucrose in phosphate buffered
saline, embedded in O.C.T. compound and stored at -80.degree. C.
Cryosections were dried, rehydrated, treated with or without
methanol, and incubated with anti-HRF (Santa Cruz biotechnology) at
4.degree. C. overnight. After washing, sections were incubated with
Alexa Fluor 647-conjugated anti-rabbit IgG (Southern Biotech), and
subsequently with Alexa Fluor 555-conjugated wheat germ agglutinin
(Invitrogen). Sections were mounted with ProLong Gold antifade
reagent with DAPI (Invitrogen). Fluorescent images were acquired
using a 31 Marianas.TM. system (Intelligent Imaging Innovations)
microscope.
Food Allergy
[0245] BALB/c mice were intraperitoneally sensitised with OVA (50
.mu.g/mouse) plus alum on days 0 and 14. From day 28, mice were
intragastrically challenged with OVA (50 mg) three times a week
(Brandt, E. B. et al. J. Clin. Invest. 112:1666-1677 (2003)).
Before each challenge, mice were starved for 3 h, then pretreated
intragastrically with GST or GST-N19 (100 .mu.g), and rested for 30
min. The development of diarrhoea was visually monitored for 90 min
after OVA challenge.
Immunofluorescence Microscopic Analysis of Location of GST-N19
[0246] Mast cells were incubated at 37.degree. C. with 20 or 200
.mu.g/ml of either TAT-GST, GST, or GST-N19 protein for 0-20 h.
Cells were washed three times with PBS and settled on slides
glasses. After fixation with 4% paraformaldehyde for 15 min, cells
were permeabilised with ice-cold methanol for 10 min., and stained
with anti-GST (Santa Cruz biotechnology), followed by Alexa Fluor
488-conjugated anti-mouse IgG (Invitrogen). ProLong Gold antifade
reagent with DAPI (Invitrogen) was used to mount the cover classes.
Fluorescence was observed with Marianas microscope system
(Intelligent Imaging Innovations).
Statistical Analysis
[0247] Differences were analyzed by one-way ANOVA and Student's t
test. The data indicated mean.+-.SEM. Single, double, and triple
asterisks indicate p<0.05, p<0.01 and p<0.001,
respectively. The incidence of diarrhea in the food allergy model
was analyzed by long-rank test. Spearman's rank correlation
coefficient was calculated for correlation between diarrhea
occurrence and numbers of mucosal mast cells.
Example 2
[0248] This example includes a description of a subset of mouse IgE
and IgG molecules, and that human IgE binds to HRF.
[0249] A previous report indicated that IgE polyclonal antibody
does not interact with HRF (Wantke, et al., J Allergy Clin Immunol
103, 642 (April 1999)). To evaluate the possibility of IgE/HRF
interactions, a panel of IgE mAbs was examined. N-terminally
glutathione S-transferase (GST)-tagged mouse HRF protein (GST-mHRF)
or GST were immobilized onto enzyme-linked immunosorbent assay
(ELISA) plates and incubated with IgE mAbs. Bound IgE was detected
with anti-IgE-biotin and streptavidin-horseradish peroxidase
conjugates. GST-mHRF, but not GST, bound C38-2, IGELa2, and 5 other
IgE mAbs (FIG. 1A). By contrast, C48-2 and 12 other IgE mAbs failed
to bind GST-mHRF using OD.sub.450<0.1 as an arbitrary cutoff
value. Similar results were obtained when mHRF-His.sub.6
(C-terminally hexahistidine-tagged mHRF) was used as a capturing
agent.
[0250] Interaction of the C38-2 and IGELa2 IgE mAbs with mHRF was
also demonstrated by co-immunoprecipitation from a mixture of IgE
and mHRF. IgEs were incubated with GST- or GST-mHRF-agarose beads.
Bead-bound IgEs were pulled down. IgE and GST proteins were
detected by immunoblotting with anti-mouse IgE antibody and
anti-GST mAb, respectively (FIG. 1B).
[0251] Binding of HRF to BMMCs preincubated with or without (gray)
the indicated IgE was assessed after cells were incubated with
mHRF-His.sub.6. Bound mHRF-His.sub.6 was detected with rabbit
anti-His tag antibody and Alexa647-conjugated anti-rabbit IgG. HRF
binding was detected by flow cytometry when WT BMMCs were incubated
with an HRF-reactive (C38-2), but not a nonreactive (C48-2 or 206),
IgE mAb. (FIG. 1C). No HRF binding was observed in C38-2
IgE-incubated Fc.epsilon.RI.alpha..sup.-/- BMMCs that lacked the
expression of the high-affinity IgE receptor, Fc.epsilon.RI. Thus,
only certain IgEs have the ability to bind mHRF.
[0252] As both HRF-reactive and HRF-nonreactive IgEs are of
allotype a, the HRF-binding site appears to reside in the variable
region of IgE. Since the variable region is shared by other Ig
isotypes, the interaction of IgG molecules with HRF was studied.
IgG molecules were incubated in GST-mHRF-coated wells. HRF-bound
IgGs were detected by ELISA. The numbers of mAbs classified into
IgG subtypes and K.sub.D values of some IgG molecules to HRF are
shown (FIG. 1D). As shown, in FIG. 1D, 9 out of the 34 tested IgG
mAbs bound to immobilized GST-mHRF by ELISA. The HRF-binding
ability of the IgG mAbs did not correlate with their antigen
specificity or isotype. For example, the HRF-binding mAbs, JK31 and
JK96, and the non-binding mAb JK116, all recognize the same viral
antigen. JK31 and JK116 belong to the IgG1 isotype, while the
HRF-binding mAbs, JK126 and JK41, and the non-HRF-binding mAbs,
HIT3a and OKT3, belong to the IgG2a isotype. Furthermore, Fab
fragments derived from HRF-binding IgE and IgG molecules were found
to bind mHRF.
[0253] Human IgE molecules were incubated in GST-mHRF-coated wells.
Bound IgEs were detected by incubation with biotin-conjugated
anti-human IgE antibody, followed by incubation with horseradish
peroxidase-conjugated streptavidin. The absorbance at 450 nm was
measured after development of the color. One of the 5 studied human
IgEs could bind GST-mHRF (FIG. 2). The results indicate that a
considerable proportion of antibodies in immunized mice and humans
recognize HRF as a superantigen. GST-hHRF (human HRF) can also bind
the Ig molecules that can bind to mHRF, as expected from the 96%
identity between hHRF and mHRF proteins.
[0254] Binding affinity (K.sub.D) between mHRF and IgGs was
measured using a quartz crystal microbalance (QCM) method (Mitomo,
et al., J Mol Recognit 20, 83 (March-April 2007)). QCM measures a
mass per unit area by measuring the change in frequency of a quartz
crystal resonator. The results showed that the affinities for three
IgG molecules are in the micromolar or submicromolar range (JK17,
0.68 .mu.M; JK31, 2.78 .mu.M; JK96, 5.78 .mu.M) (FIG. 1D).
Example 3
[0255] This example includes a description of studies indicating
that the N-terminal 19 residue peptide of HRF inhibits the HRF/IgE
interaction.
[0256] To map the Ig-interaction site within the mHRF molecule, an
ELISA panel of N- or C-terminally truncated GST-mHRF proteins (FIG.
3A) was used as capturing agents and two HRF-reactive IgE mAbs
(C38-2 and aOE) and two HRF-reactive IgG mAbs (JK18 and JK31) as
binding probes. C38-2 and aOE IgE molecules were incubated in wells
coated with full-length or truncated forms of GST-mHRF. After
blocking, JK18 and JK31 IgGs were incubated. The bound IgGs were
detected by incubation with horseradish peroxidase-conjugated
anti-mouse IgG antibody. The absorbance at 450 nm was measured
after development of the color.
[0257] HRF-reactive IgE and IgG mAbs gave similar binding patterns
(FIGS. 3B and 4). C-terminal truncations of mHRF up to residue 19
did not affect Ig binding, but a further truncation abrogated Ig
binding. Thus, a major Ig-binding site was mapped to the N-terminal
part of HRF (residues 1-19). A comparison between GST-N78 and other
C-terminally truncated HRF constructs showed that retention of the
HRF sequence from position 79 to position 142 or 154 reduces Ig
binding. Thus, this HRF sequence inhibits Ig binding via the
N-terminal binding site. Binding assays using N-terminally
truncated proteins, 79-172 and 79-142, indicated that another
Ig-binding site is present at the internal part of HRF (residues
79-142) and that the C-terminal fragment of HRF (residues 155-172)
prevents this internal segment of HRF from interacting with the
Igs. Thus, HRF can interact with the IgE and IgG molecules via at
least two interaction sites with the mHRF's N-terminal interaction
site functioning as a predominant interaction site.
Example 4
[0258] This example includes a description of studies indicating
that GST-N19 does not affect the growth or apoptosis in various
cells.
[0259] To identify an inhibitor of HRF/Ig interactions to dissect
the extracellular function (.dbd.HRF function as a cytokine)
separate from the intracellular functions in in vivo settings, the
major Ig-interacting HRF sequence was studied as a specific
inhibitor of HRF/Ig interactions. IgE molecules were incubated in
GST-mHRF-coated wells in the presence or absence (-) of the
indicated concentrations of competitors, GST-N19, GST-mHRF or
mHRF-His.sub.6 for 2 hours. After incubation, bound-IgE was
detected by incubation with biotinylated anti-mouse IgE mAb,
followed by streptavidin-HRP. GST-N19 (encoding the first 19
residues of mHRF; designated N19 in FIG. 3A) inhibited IgE binding
to immobilized GST-mHRF by ELISA with potency similar to that shown
by GST-mHRF and mHRF-His.sub.6 (FIG. 3C). By contrast, none of the
shorter mHRF peptides tested (residues 1-6, 1-12, 5-19, and 9-19)
inhibited HRF/IgE binding. GST-N19 inhibited HRF/IgG binding as
well.
[0260] To analyze the effect of GST-N19 on intracellular functions
of HRF/TCTP, the indicated cells were cultured in the absence (-)
or presence of the indicated concentrations of GST or GST-N19 for 2
days, and live cells were counted in the presence of Trypan blue.
Treatment of the cultures of BMMCs, CHO-K.sup.1 (Chinese hamster
ovary cell), Jurkat (human T cell), Caco-2 (human colonic
epithelial cell), A549 (human lung epithelial cell), and HeLa
(human cervical epidermal cell) cells with 3.6 or 36 .mu.M of
GST-N19, did not affect viability or proliferation of either cells
(FIG. 5A, live cells shown by the exclusion of Trypan blue). The
usual dose of HRF to stimulate basophils is 1.6-5 .mu.M (Langdon,
et al., J Leukoc Biol 84, 1151 (October, 2008)). Apoptosis was
induced by growth factor withdrawal, IL-3 depletion, in BMMCs for 3
days and by 800 nM H.sub.2O.sub.2 in CHO-K.sup.1 cells for 2 days,
and live cells were counted. The CHO-K.sup.1 cells were not
affected by GST-N19 (FIG. 5B). Furthermore, confocal microscopy
showed that GST-N19 does not enter the interior of cells.
[0261] These results indicate that N19 peptide can be used to probe
the specific in vivo function of HRF/Ig interactions or the HRF
function as an autoantigen. All these and subsequent in vitro and
in vivo studies were performed using recombinant proteins
(mHRF-His.sub.6, GST, and GST-N19) that contained <0.05 pg/.mu.g
protein of endotoxin.
Example 5
[0262] This example includes a description of studies indicating
that HRF can induce weak acute and strong late-phase anaphylactic
cutaneous reactions in HRF-reactive IgE-sensitized mice.
[0263] Acute passive cutaneous anaphylaxis (PCA) reactions, a
typical type I hypersensitivity, are usually induced by multivalent
antigen in IgE-sensitized mice and mediated mainly by histamine
released from activated mast cells, while the late-phase reactions
(LPR) in the skin is mediated in part by TNF-.alpha. secreted from
activated mast cells (Wershil, et al., J Clin Invest 87, 446
(February 1991), Nagai et al., Inflamm Res 45, 136 (March, 1996)).
Here, mice were sensitized with the IgE. Twenty four hours later,
Evans blue was intravenously injected and mHRF-His.sub.6 was
consecutively injected in IgE-sensitized ears. After 30 minutes,
mice were sacrificed, and the amount of Evans blue dye leaked from
the ears measured. For negative and positive controls, saline (Sal)
and TNP.sub.26-BSA (Ag), respectively, were injected in sensitized
ears. In addition to C57BL/6 (WT), B6-Kit.sup.W-sh/W-sh mice were
used before or 6 weeks after engraftment of WT BMMCs by intradermal
injection. Toluidine Blue staining confirmed that the engrafted
mice had levels of mast cells similar to those of WT mice. The
intradermal injection of mHRF 24 hours after IgE injection showed
that both the acute reactions and LPR are induced by HRF-reactive,
but not HRF-nonreactive, IgEs (FIG. 6). The HRF-reactive C38-2 IgE,
but not the nonreactive C48-2 IgE, induced increased vascular
permeability measured at 30 minutes after mHRF injection (FIG.
6A).
[0264] mHRF-His.sub.6 (H) was injected in IgE-sensitized ears and
LPR was analyzed by measurement of ear thickness at 8 hours after
injection. For negative and positive controls, saline (S) and
TNP.sub.26-BSA (Ag), respectively, were injected in sensitized
ears. Interestingly, the LPR induced by HRF, as measured by
increased ear swelling at 8 hours, was as high as that induced by
antigen (FIG. 6C).
[0265] Similar weak acute and strong late-phase responses were
observed using another HRF-reactive IgE, IGELa2, in both C57BL/6
and BALB/c mice (FIG. 7). IGELa2 IgE was intradermally injected
into ears. After overnight sensitization, the mice were challenged
by intradermal injection of saline (Sal), antigen (Ag), or HRF.
Eight hours after injection, the ear thickness was measured (FIG.
7).
[0266] These hypersensitivity reactions appear to be dependent on
mast cells, as the reactions were abolished in mast cell-deficient
Kit.sup.W-sh/W-sh mice and restored in Kit.sup.W-sh/W-sh mice
engrafted with WT BMMCs (FIG. 6A). Furthermore,
Fc.epsilon.RIa.sup.-/- mice showed neither acute or late-phase
responses (FIG. 6B). Therefore, HRF and HRF-reactive IgE can induce
anaphylactic responses in a mast cell- and Fc.epsilon.RI-dependent
manner. Consistent with this, peritoneal mast cells sensitized with
the HRF-reactive, but not the HRF-nonreactive, IgE were activated
upon stimulation with HRF (FIG. 6D). After 40 minutes, both
supernatants and cell pellets were collected and
.beta.-hexosaminidase release was analyzed (FIG. 6D). Similar weak
acute and strong late-phase responses were also induced by HRF and
HRF-reactive IgG.
[0267] To study HRFs involvement in IgE/antigen-induced PCA
reactions, WT mice were overnight sensitized the IgE. Left ears
were injected with GST and the right ears were injected with
GST-N19, and then TNP.sub.26-BSA were injected in both ears. Ear
thickness was measured over a 10 hour period. *, **, ***:
p<0.05, p<0.01, p<0.001 by Student's t-test. As shown in
FIG. 6E, both acute reactions and LPR in an HRF-reactive
IgE-induced PCA were significantly reduced by GST-N19, but not GST.
However, PCA induced by an HRF-nonreactive IgE was insensitive to
GST-N19.
Example 6
[0268] This example includes a description of studies indicating
that HRF is involved as a superantigen in amplifying airway
inflammation.
[0269] Asthma is a type 2 helper T cell-driven, chronic
inflammatory lung disease (Wills-Karp, Annu Rev Immunol 17, 255
(1999)). To study HRF involvement in allergic airway inflammation,
an airway inflammation model was utilized, in which mast cells are
implicated in amplifying antigen-dependent chronic inflammation
(Williams, et al., J Exp Med 192, 455 (Aug. 7, 2000)). C57BL/6 mice
were first sensitized with OVA (10 .mu.g) at days 0, 7, 14, 21, 28
and 35. At days 40, 43 and 46, mice were intranasally challenged
with OVA (20 .mu.g) or PBS. Some mice were intranasally pretreated
with GST or GST-N19 (400 .mu.g) before every OVA challenge.
Twenty-four hours after the last challenge, mice were sacrificed
and BAL fluids and lung tissues were collected. Lung tissues were
homogenized. In addition to the increased HRF levels in lung
tissues, BAL fluids, and plasma of OVA-immunized/OVA challenged
mice (FIGS. 8A and 9A; plasma HRF: 0.367.+-.0.09 .mu.g/ml in
PBS-treated mice vs. 1.32.+-.0.13 .mu.g/ml in OVA-challenged mice,
p=0.0036), immunofluorescence microscopy of lung sections showed
increased levels of HRF staining in non-permeabilized lung tissues
(FIG. 8B), indicating that HRF is secreted into the lung tissues in
OVA-immunized/OVA-challenged mice. Levels of HRF-reactive IgG were
also increased in plasma and BAL fluids of these mice (FIG. 9B),
while HRF-reactive IgE was under detection limits in our assays.
Treatment with GST-N19 before each OVA challenge abrogated airway
inflammation, as evidenced by reduced inflammatory cells,
particularly, eosinophils and neutrophils, in BAL fluids (FIG. 8C)
and by reduced inflammatory cells and goblet cell hyperplasia in
the lung (FIG. 8D). Production of IL-13 (the cytokine essential for
airway hyperresponsiveness, eosinophilia and mucus production
(Wills-Karp, et al., Science 282, 2258 (Dec. 18, 1998), Grunig, et
al., Science 282, 2261 (Dec. 18, 1998)) and IL-5 (the cytokine
critical for eosinophilia and airway hyperresponsiveness (Foster,
et al., J Exp Med 183, 195 (Jan. 1, 1996)) in lung tissues was
drastically decreased in GST-N19-treated mice (FIG. 8E). Consistent
with these observations, GST-N19 treatment inhibited AHR (FIG. 8F).
Furthermore, the increased HRF in plasma in
OVA-immunized/OVA-challenged mice were reduced by GST-N19 (FIG.
9A). By contrast, HRF-reactive IgG levels (FIG. 9B) or OVA-specific
IgE, IgG1, or IgG2a levels (FIG. 10) in plasma were not affected by
GST-N19. Administration of the synthetic non-fusion N19 peptide
also abrogated airway inflammation (FIG. 11A-11C). By contrast,
GST-N19 failed to inhibit airway inflammation in a T
cell-dependent/mast cell-independent model (Takeda, et al., J Exp
Med 186, 449 (1997)), in which mice were immunized with OVA with
alum and challenged with OVA (FIGS. 11D and 11E). The efficacy of
GST-N19 was confirmed in another model of asthma, one induced by
Aspergillus fumigatus allergens (FIG. 12), which induced dramatic
expansion and activation of airway mast cells and recruitment of
eosinophils in an IgE-dependent manner. These results strongly
indicate that HRF is critically involved as a superantigen in
amplifying airway inflammation.
Example 7
[0270] This example includes a description of studies indicating
that weak lung inflammation is induced by HRF alone in naive
mice.
[0271] In light of the presence of HRF-reactive IgG in naive mice
and recent studies reporting the role of mast cells in the
initiation of adaptive immune responses (Galli, et al., Nat Immunol
6, 135 (February, 2005)), the effect of HRF on naive mice was
assessed. Unimmunized C57BL/6 (WT), .mu.MT, and
Fc.epsilon.R.gamma..sup.-/- mice (1=5 per cohort) were treated
intranasally with 40 .mu.g mHRF-His.sub.6 three times every third
day. PBS served as a negative control. BAL procedures were
conducted 24 hours after the last HRF administration. Differential
cell counting was performed on cytospin preparations stained with
May-Giemsa. Intranasal administration of mHRF-His.sub.6 induced
weak lung inflammation, as shown by increased neutrophils and
eosinophils in BAL fluids (FIG. 13). HRF effects were not observed
in B cell-deficient (.mu.MT) or Fc.epsilon.R.gamma..sup.-/- mice,
suggesting that HRF requires Igs and Fc receptors for its
proinflammatory function. FcRy is shared by multiple Fc receptors
including Fc.epsilon.RI. Among the candidate Igs and Fc receptors,
IgE and its receptor, Fc.epsilon.RI, seemed to play a predominant
role, as HRF-induced lung inflammation was abrogated or reduced in
naive Fc.epsilon.RI.alpha..sup.-/- mice. Since Fc.epsilon.RI is
expressed only in mast cells and basophils in mice, these results
are consistent with the effectiveness of N19 peptide in mast
cell-dependent asthma models. The failed increase of inflammatory
cells in BAL fluids of HRF-challenged naive .mu.MT or
FcR.gamma..sup.-/- mice suggests that HRF does not have receptors
other than Igs.
Example 8
[0272] This example includes a description of studies indicating
that N19 peptide pretreatment prevents development of diarrhea in a
mouse model of food allergy.
[0273] To evaluate the role of HRF in food allergy, an OVA-induced
food allergy model developed by Brandt et al. (Brandt et al., J.
Clin. Invest. 112:1666 (2003)) was employed. BALB/c mice that were
6-8-week-old were intraperitoneal (i.p.) immunized twice on days 0
and 14 with 50 .mu.g of OVA in the presence of alum. From day 28,
mice were intragastrically challenged with OVA (50 mg) three times
a week. Before each challenge, mice were starved for 3 hours, then
pretreated with GST or GST-N19 (100 .mu.g/mouse) intragastrically,
and rested for 30 minutes. Control mice were sensitized and
challenged, but not pretreated. The development of diarrhea was
monitored for ninety minutes after OVA challenge. Visual monitoring
following each intragastric OVA challenge showed the induction of
diarrhea in OVA-immunized, but not PBS control mice (FIG. 14).
Sections of jejunum and ileum from mice treated as in FIG. 14 were
stained with chloroacetate esterase. These sections of jejunum and
ileum revealed increased mucosal mast cells in mice (FIG. 15A-C).
There was a good correlation shown between the number of mucosal
mast cells and the number of diarrhea occurrence during 10 OVA
challenges (FIG. 15D). Increased serum IgE and IgG1, but not IgG2a,
levels (FIG. 16) and increased expression of mRNAs encoding Th2
cytokines (IL-4, IL-5, IL-13) and proinflammatory cytokines (IL-6,
IL-113) in the intestines were observed (data not shown). In this
Th2-skewed model, the amount of HRF in plasma from diarrhea-induced
mice, as measured by immunoblotting, was increased by about 2 fold
compared to PBS control mice (FIG. 16E). HRF-reactive IgG levels
were also increased in sera of diarrhea-induced mice (FIG. 16D).
However, HRF-reactive IgE was not detected under the study
conditions.
[0274] Importantly, the development of diarrhea was prevented in a
much greater percentage of mice pretreated with GST-N19 than in
GST-pretreated or PBS-treated control mice (FIG. 14). Increases in
both mucosal and serosal mast cells were inhibited by GST-N19
pretreatment (FIG. 15A-C). However, plasma levels of total IgE,
total IgG1, total IgG2a, or HRF-reactive IgG were not affected by
GST-N19 (FIG. 16). These results indicate that HRF is involved in
OVA-induced diarrhea induction and mast cell accumulation in the
small intestine. Given the absence of GST-N19 effects on mast cell
growth and apoptosis, the inhibition of mast cell accumulation in
GST-N19-treated mice might be due to reduced recruitment of mast
cells in the inflamed intestines.
Example 9
[0275] This example includes a description of studies indicating
that human food allergy patients with high serum IgE levels tend to
have increased levels of serum HRF and HRF-reactive IgG.
[0276] To determine if the histamine-releasing activity associated
with food allergy, which was described 20 years ago (Sampson et
al., N. Engl. J. Med. 321:228 (1989)), is due to HRF, serum
HRF-reactive IgG levels in small cohorts (1=4 or 6 per cohort) of
food allergy patients with increased serum food-specific IgE who
showed anaphylaxis or not, and food allergy patients with no
increase in food-specific serum IgE, was measured. There were
small, although statistically not significant, increases in
HRF-reactive IgG levels in patients with high food-specific IgE
levels (FIG. 17). The results indicate that food allergy patients
tend to have higher levels of serum HRF and HRF-reactive IgG.
Example 10
[0277] This example includes a description of studies indicating
that an HRF-reactive IgE can bind both monomeric and dimeric forms
of HRF.
[0278] HRF binding to IgE (or IgG) bound to Fc receptors activates
immune cells such as mast cells and basophils. A potential cell
activation mechanism by HRF is through HRF interactions with two or
more Ig molecules and/or multimerization of HRF molecules, both
leading to crosslinking Ig-bound Fc receptors. The former was
indicated by the above-mentioned mapping results (FIG. 3B).
Analysis of purified recombinant mHRF-His.sub.6 on reducing and
non-reducing SDS-PAGE yielded direct evidence for dimerization of
mHRF (FIG. 18). In this study (FIG. 18), mHRF-His.sub.6 protein
purified with ProBond.TM. nickel-chelating resin (Invitrogen) was
analyzed by SDS-PAGE under reducing (100 mM DTT) or non-reducing
conditions and stained with Coomassie Brilliant Blue. Size
exclusion chromatography also indicated that the mHRF protein
consists of disulfide-linked homodimers and monomers.
[0279] Monomeric and dimeric HRF-His.sub.6 proteins purified by
HPLC were coated onto plastic wells. After blocking, 206
(HRF-nonreactive) and IGELa2 (HRFreactive) IgEs were incubated.
Bound IgEs were detected by incubation with biotin-conjugated
anti-mouse IgE antibody, followed by incubation with horseradish
peroxidase-conjugated streptavidin. The absorbance at 450 nm was
measured after development of the color. This ELISA using purified
mHRF monomers or dimers as a capturing agent showed that both
monomeric and dimeric forms of mHRF can bind to IgE (FIG. 19). The
dimerizing ability of HRF with multiple Ig-binding sites strongly
indicates the potential of HRF to crosslink Ig-bound Fc
receptors.
[0280] HRF functions as an Ig-interacting pro-inflammatory factor
with properties similar to B cell superantigens that can activate
mast cells and basophils through antibodies bound to their
membrane-bound Fc receptors (Patella et al., J. Immunol. 145:3054
(1990); Patella et al., J. Immunol. 164:589 (2000); Patella et al.,
Int. Arch. Allergy Immunol. 118:197 (1999)). Consistent with the
notion that HRF could be a B cell superantigen (Silverman and
Goodyear, Nat. Rev. Immunol. 6:465 (2006)), HRF binds to a
substantial proportion of Igs (this study) and HRF can promote B
cell proliferation (Kang et al., J. Immunol. 166:6545 (2001)). We
propose that similar to B cell superantigens, which activate target
cells by oligomerization of antigen receptors, interaction of
dimeric HRF with Fc receptor-bound Igs on mast cells and other
immune cells will lead to their activation (FIG. 20).
Sequence CWU 1
1
24119PRTHomo sapiens 1Met Ile Ile Tyr Arg Asp Leu Ile Ser His Asp
Glu Met Phe Ser Asp 1 5 10 15 Ile Tyr Lys 264PRTHomo sapiens 2Gln
Glu Thr Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp 1 5 10
15 Tyr Met Lys Ser Ile Lys Gly Lys Leu Glu Glu Gln Arg Pro Glu Arg
20 25 30 Val Lys Pro Phe Met Thr Gly Ala Ala Glu Gln Ile Lys His
Ile Leu 35 40 45 Ala Asn Phe Lys Asn Tyr Gln Phe Phe Ile Gly Glu
Asn Met Asn Pro 50 55 60 3172PRTHomo sapiens 3Met Ile Ile Tyr Arg
Asp Leu Ile Ser His Asp Glu Met Phe Ser Asp 1 5 10 15 Ile Tyr Lys
Ile Arg Glu Ile Ala Asp Gly Leu Cys Leu Glu Val Glu 20 25 30 Gly
Lys Met Val Ser Arg Thr Glu Gly Asn Ile Asp Asp Ser Leu Ile 35 40
45 Gly Gly Asn Ala Ser Ala Glu Gly Pro Glu Gly Glu Gly Thr Glu Ser
50 55 60 Thr Val Ile Thr Gly Val Asp Ile Val Met Asn His His Leu
Gln Glu 65 70 75 80 Thr Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile
Lys Asp Tyr Met 85 90 95 Lys Ser Ile Lys Gly Lys Leu Glu Glu Gln
Arg Pro Glu Arg Val Lys 100 105 110 Pro Phe Met Thr Gly Ala Ala Glu
Gln Ile Lys His Ile Leu Ala Asn 115 120 125 Phe Lys Asn Tyr Gln Phe
Phe Ile Gly Glu Asn Met Asn Pro Asp Gly 130 135 140 Met Val Ala Leu
Leu Asp Tyr Arg Glu Asp Gly Val Thr Pro Tyr Met 145 150 155 160 Ile
Phe Phe Lys Asp Gly Leu Glu Met Glu Lys Cys 165 170 4172PRTHomo
sapiens 4Met Ile Ile Tyr Arg Asp Leu Ile Ser His Asp Glu Met Phe
Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg Glu Ile Ala Asp Gly Leu Cys
Leu Glu Val Glu 20 25 30 Gly Lys Met Val Ser Arg Thr Glu Gly Asn
Ile Asp Asp Ser Leu Ile 35 40 45 Gly Gly Asn Ala Ser Ala Glu Gly
Pro Glu Gly Glu Gly Thr Glu Ser 50 55 60 Thr Val Ile Thr Gly Val
Asp Ile Val Met Asn His His Leu Gln Glu 65 70 75 80 Thr Ser Phe Thr
Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp Tyr Met 85 90 95 Lys Ser
Ile Lys Gly Lys Leu Glu Glu Gln Arg Pro Glu Arg Val Lys 100 105 110
Pro Phe Met Thr Gly Ala Ala Glu Gln Ile Lys His Ile Leu Ala Asn 115
120 125 Phe Lys Asn Tyr Gln Phe Phe Ile Gly Glu Asn Met Asn Pro Asp
Gly 130 135 140 Met Val Ala Leu Leu Asp Tyr Arg Glu Asp Gly Val Thr
Pro Tyr Met 145 150 155 160 Ile Phe Phe Lys Asp Gly Leu Glu Met Glu
Lys Cys 165 170 5172PRTMus musculus 5Met Ile Ile Tyr Arg Asp Leu
Ile Ser His Asp Glu Leu Phe Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg
Glu Ile Ala Asp Gly Leu Cys Leu Glu Val Glu 20 25 30 Gly Lys Met
Val Ser Arg Thr Glu Gly Ala Ile Asp Asp Ser Leu Ile 35 40 45 Gly
Gly Asn Ala Ser Ala Glu Gly Pro Glu Gly Glu Gly Thr Glu Ser 50 55
60 Thr Val Val Thr Gly Val Asp Ile Val Met Asn His His Leu Gln Glu
65 70 75 80 Thr Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp
Tyr Met 85 90 95 Lys Ser Leu Lys Gly Lys Leu Glu Glu Gln Lys Pro
Glu Arg Val Lys 100 105 110 Pro Phe Met Thr Gly Ala Ala Glu Gln Ile
Lys His Ile Leu Ala Asn 115 120 125 Phe Asn Asn Tyr Gln Phe Phe Ile
Gly Glu Asn Met Asn Pro Asp Gly 130 135 140 Met Val Ala Leu Leu Asp
Tyr Arg Glu Asp Gly Val Thr Pro Phe Met 145 150 155 160 Ile Phe Phe
Lys Asp Gly Leu Glu Met Glu Lys Cys 165 170 6172PRTRattus
Norvegious 6Met Ile Ile Tyr Arg Asp Leu Ile Ser His Asp Glu Leu Phe
Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg Glu Ile Ala Asp Gly Leu Cys
Leu Glu Val Glu 20 25 30 Gly Lys Met Val Ser Arg Thr Glu Gly Ala
Ile Asp Asp Ser Leu Ile 35 40 45 Gly Gly Asn Ala Ser Ala Glu Gly
Pro Glu Gly Glu Gly Thr Glu Ser 50 55 60 Thr Val Val Thr Gly Val
Asp Ile Val Met Asn His His Leu Gln Glu 65 70 75 80 Thr Ser Phe Thr
Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp Tyr Met 85 90 95 Lys Ser
Leu Lys Gly Lys Leu Glu Glu Gln Lys Pro Glu Arg Val Lys 100 105 110
Pro Phe Met Thr Gly Ala Ala Glu Gln Ile Lys His Ile Leu Ala Asn 115
120 125 Phe Asn Asn Tyr Gln Phe Phe Ile Gly Glu Asn Met Asn Pro Asp
Gly 130 135 140 Met Val Ala Leu Leu Asp Tyr Arg Glu Asp Gly Val Thr
Pro Phe Met 145 150 155 160 Ile Phe Phe Lys Asp Gly Leu Glu Met Glu
Lys Cys 165 170 7172PRTLeporidae 7Met Ile Ile Tyr Arg Asp Leu Ile
Ser His Asp Glu Met Phe Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg Glu
Ile Ala Gly Gly Leu Cys Leu Glu Val Glu 20 25 30 Gly Lys Met Val
Ser Arg Thr Glu Gly Asn Ile Asp Asp Ser Leu Ile 35 40 45 Gly Gly
Asn Ala Ser Ala Glu Gly Pro Glu Gly Glu Gly Thr Glu Ser 50 55 60
Thr Val Ile Thr Gly Val Asp Ile Val Met Asn His His Leu Gln Glu 65
70 75 80 Thr Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp
Tyr Met 85 90 95 Lys Ser Ile Lys Gly Lys Leu Glu Glu Gln Arg Pro
Glu Arg Val Lys 100 105 110 Pro Phe Met Thr Gly Ala Ala Glu Gln Ile
Lys His Ile Leu Ala Asn 115 120 125 Phe Lys Asn Tyr Gln Phe Tyr Ile
Gly Glu Asn Met Asn Pro Asp Gly 130 135 140 Met Val Ala Leu Leu Asp
Tyr Arg Glu Asp Gly Val Thr Pro Phe Met 145 150 155 160 Ile Phe Phe
Lys Asp Gly Leu Glu Met Glu Lys Cys 165 170 8172PRTCavia Porcellus
8Met Ile Ile Tyr Arg Asp Leu Ile Ser His Asp Glu Met Phe Ser Asp 1
5 10 15 Ile Tyr Lys Ile Arg Glu Ile Ala Asp Gly Leu Cys Leu Glu Val
Glu 20 25 30 Gly Lys Met Val Ser Arg Thr Glu Gly Asn Ile Asp Asp
Ser Leu Ile 35 40 45 Gly Gly Asn Ala Ser Ala Glu Gly Pro Glu Gly
Glu Gly Thr Glu Ser 50 55 60 Thr Val Ile Thr Gly Val Asp Ile Val
Met Asn His His Leu Gln Glu 65 70 75 80 Thr Ser Phe Thr Lys Glu Ala
Tyr Lys Lys Tyr Ile Lys Asp Tyr Met 85 90 95 Lys Ser Ile Lys Gly
Lys Leu Glu Glu Gln Arg Pro Glu Arg Val Lys 100 105 110 Pro Phe Met
Thr Gly Ala Ala Glu Gln Ile Lys His Ile Leu Ala Asn 115 120 125 Phe
Lys Asn Tyr Gln Phe Phe Ile Gly Ala Asn Met Asn Pro Asp Gly 130 135
140 Met Val Ala Leu Leu Asp Tyr Arg Glu Asp Gly Val Thr Pro Phe Met
145 150 155 160 Ile Phe Phe Lys Asp Gly Leu Glu Met Glu Lys Cys 165
170 9172PRTPan Troglodytes 9Met Ile Ile Tyr Arg Asp Leu Ile Ser His
Asp Glu Met Phe Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg Glu Ile Ala
Asp Gly Leu Cys Leu Glu Val Glu 20 25 30 Gly Lys Met Val Ser Arg
Thr Glu Gly Asn Ile Asp Asp Ser Leu Ile 35 40 45 Gly Gly Asn Ala
Ser Ala Glu Gly Pro Glu Gly Glu Gly Thr Glu Ser 50 55 60 Thr Val
Ile Thr Gly Val Asp Ile Val Met Asn His His Leu Gln Glu 65 70 75 80
Thr Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp Tyr Met 85
90 95 Lys Ser Ile Lys Gly Lys Leu Glu Glu Gln Arg Pro Glu Arg Val
Lys 100 105 110 Pro Phe Met Thr Gly Ala Ala Glu Gln Ile Lys His Ile
Leu Ala Asn 115 120 125 Phe Lys Asn Tyr Gln Phe Phe Ile Gly Glu Asn
Met Asn Pro Asp Gly 130 135 140 Met Val Ala Leu Leu Asp Tyr Arg Glu
Asp Gly Val Thr Pro Tyr Met 145 150 155 160 Ile Phe Phe Lys Asp Gly
Leu Glu Met Glu Lys Cys 165 170 10171PRTCercopithecoid 10Met Ile
Ile Tyr Arg Asp Leu Ile Ser His Asp Glu Met Phe Ser Asp 1 5 10 15
Ile Tyr Lys Ile Arg Glu Ile Ala Asp Gly Leu Cys Leu Glu Val Glu 20
25 30 Gly Lys Val Ser Arg Thr Glu Gly Asn Ile Asp Asp Ser Leu Ile
Gly 35 40 45 Gly Asn Ala Ser Ala Glu Gly Pro Glu Gly Glu Gly Thr
Glu Ser Thr 50 55 60 Val Ile Thr Gly Val Asp Ile Val Met Asn His
His Leu Gln Glu Thr 65 70 75 80 Ser Phe Thr Lys Glu Ala Tyr Lys Lys
Tyr Ile Lys Asp Tyr Met Lys 85 90 95 Ser Ile Lys Gly Lys Leu Glu
Glu Gln Arg Pro Glu Arg Val Lys Pro 100 105 110 Phe Met Thr Gly Ala
Ala Glu Gln Ile Lys His Ile Leu Ala Asn Phe 115 120 125 Lys Asn Tyr
Gln Phe Phe Ile Gly Glu Asn Met Asn Pro Asp Gly Met 130 135 140 Val
Ala Leu Leu Asp Tyr Arg Glu Asp Gly Val Thr Pro Tyr Met Ile 145 150
155 160 Phe Phe Lys Asp Gly Leu Glu Met Glu Lys Cys 165 170
11172PRTCanis Lupus familiaris 11Met Ile Ile Tyr Arg Asp Leu Ile
Ser His Asp Glu Met Phe Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg Glu
Ile Ala Asp Gly Leu Cys Leu Glu Val Glu 20 25 30 Gly Lys Met Val
Ser Arg Thr Glu Gly Asn Ile Asp Asp Ser Leu Ile 35 40 45 Gly Gly
Asn Ala Ser Ala Glu Gly Pro Glu Gly Glu Gly Thr Glu Ser 50 55 60
Thr Val Ile Thr Gly Val Asp Ile Val Met Asn His His Leu Gln Glu 65
70 75 80 Thr Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp
Tyr Met 85 90 95 Lys Ser Ile Lys Gly Lys Leu Glu Glu Gln Arg Pro
Glu Arg Val Lys 100 105 110 Pro Phe Met Thr Gly Ala Ala Glu Gln Ile
Lys His Ile Leu Ala Asn 115 120 125 Phe Lys Asn Tyr Gln Phe Phe Ile
Gly Glu Asn Met Asn Pro Asp Gly 130 135 140 Met Val Ala Leu Leu Asp
Tyr Arg Glu Asp Gly Val Thr Pro Tyr Met 145 150 155 160 Ile Phe Phe
Lys Asp Gly Leu Glu Met Glu Lys Cys 165 170 12172PRTsus Suinae
12Met Ile Ile Tyr Arg Asp Leu Ile Ser His Asp Glu Met Phe Ser Asp 1
5 10 15 Ile Tyr Lys Ile Arg Glu Ile Ala Asp Gly Leu Cys Leu Glu Val
Glu 20 25 30 Gly Lys Met Val Ser Arg Thr Glu Gly Asn Ile Asp Asp
Ser Leu Ile 35 40 45 Gly Gly Asn Ala Ser Ala Glu Gly Pro Glu Gly
Glu Gly Thr Glu Ser 50 55 60 Thr Val Ile Thr Gly Val Asp Ile Val
Met Asn His His Leu Gln Glu 65 70 75 80 Thr Ser Phe Thr Lys Glu Ala
Tyr Lys Lys Tyr Ile Lys Asp Tyr Met 85 90 95 Lys Ser Ile Lys Gly
Lys Leu Glu Glu Gln Arg Pro Glu Arg Val Lys 100 105 110 Pro Phe Met
Thr Gly Ala Ala Glu Gln Ile Lys His Ile Leu Ala Asn 115 120 125 Phe
Lys Asn Tyr Gln Phe Phe Ile Gly Glu Asn Met Asn Pro Asp Gly 130 135
140 Met Val Ala Leu Leu Asp Tyr Arg Glu Asp Gly Val Thr Pro Tyr Met
145 150 155 160 Ile Phe Phe Lys Asp Gly Leu Glu Met Glu Lys Cys 165
170 13172PRTBovine 13Met Ile Ile Tyr Arg Asp Leu Ile Ser His Asp
Glu Met Phe Ser Asp 1 5 10 15 Ile Tyr Lys Ile Arg Glu Val Ala Asp
Gly Leu Cys Leu Glu Val Glu 20 25 30 Gly Lys Met Val Ser Arg Thr
Glu Gly Asn Ile Asp Asp Ser Leu Ile 35 40 45 Gly Gly Asn Ala Ser
Ala Glu Gly Pro Glu Gly Glu Gly Thr Glu Ser 50 55 60 Thr Val Ile
Thr Gly Val Asp Ile Val Met Asn His His Leu Gln Glu 65 70 75 80 Thr
Ser Phe Thr Lys Glu Ala Tyr Lys Lys Tyr Ile Lys Asp Tyr Met 85 90
95 Lys Ser Ile Lys Gly Lys Leu Glu Glu Gln Arg Pro Glu Arg Val Lys
100 105 110 Pro Phe Met Thr Gly Ala Ala Glu Gln Ile Lys His Ile Leu
Ala Asn 115 120 125 Phe Lys Asn Tyr Gln Phe Phe Ile Gly Glu Asn Met
Asn Pro Asp Gly 130 135 140 Met Val Ala Leu Leu Asp Tyr Arg Glu Asp
Gly Val Thr Pro Tyr Met 145 150 155 160 Ile Phe Phe Lys Asp Gly Leu
Glu Met Glu Lys Cys 165 170 1429DNAArtificial SequenceDescription
of Artifical Sequence Forward primer for GST fusion proteins
14aaaaggatcc atgatcatct accgggacc 291530DNAArtificial
SequenceDescription of Artifical Sequence Forward primer for GST
fusion proteins 15aaaaggatcc caagaaacca gcttcacaaa
301627DNAArtificial SequenceDescription of Artifical Sequence
Reverse primer for GST fusion proteins 16tttgaattct tactcgtcat
ggctgat 271732DNAArtificial SequenceDescription of Artifical
Sequence Reverse primer for GST fusion proteins 17aaagaattct
tacttgtaga tgtcggagaa ca 321832DNAArtificial SequenceDescription of
Artifical Sequence Reverse primer for GST fusion proteins
18aaagaattct tactccacct ccaggcacag cc 321932DNAArtificial
SequenceDescription of Artifical Sequence Reverse primer for GST
fusion proteins 19aaagaattct tagagcgagt catcgatggc ac
322032DNAArtificial SequenceDescription of Artifical Sequence
Reverse primer for GST fusion proteins 20aaagaattct tataagtgat
ggttcatgac aa 322132DNAArtificial SequenceDescription of Artifical
Sequence Reverse primer for GST fusion proteins 21aaagaattct
tatggattca tgttttcacc aa 322230DNAArtificial SequenceDescription of
Artifical Sequence Reverse primer for GST fusion proteins
22aaaaggatcc caagaaacca gcttcacaaa 302327DNAArtificial
SequenceDescription of Artifical Sequence Forward primer for
His6-tagged mouse HRF 23aaaacatatg atcatctacc gggacct
272430DNAArtificial SequenceDescription of Artifical Sequence
Reverse primer for His6-tagged mouse HRF 24ttttctcgag acatttctcc
atctctaagc 30
* * * * *