U.S. patent application number 13/655278 was filed with the patent office on 2013-02-14 for vaccine comprising an attenuated pestivirus. This patent application is currently assigned to BOEHRINGER INGELHEIM VETMEDICA GMBH. The applicant listed for this patent is Boehringer Ingelheim Vetmedica Gmbh. Invention is credited to Andreas EGE, Christiane MEYER, Gregor MEYERS, Martina von FREYBURG.
Application Number | 20130039946 13/655278 |
Document ID | / |
Family ID | 35506051 |
Filed Date | 2013-02-14 |
United States Patent Application | 20130039946 |
Kind Code | A1 |
MEYERS; Gregor ; et al. | February 14, 2013 |
Attenuated pestiviruses, in particular attenuated BVDV, wherein at least one mutation is in the coding sequence for glycoprotein E.sup.ms and at least another mutation in the coding sequence for N.sup.pro which preferably leads to combined inactivation of the RNase activity residing in glycoprotein E.sup.ms in addition to the inactivation of the (hypothesized) immunomodulating activity residing in N.sup.pro. Methods for attenuating pestiviruses such as BVDV, nucleic acids encoding the pestiviruses, in particular BVDV, compositions and vaccines comprising the attenuated pestiviruses, in particular BVDV, of the invention.
Inventors: | MEYERS; Gregor; (Waldorfhaeslach, DE) ; EGE; Andreas; (Teubingen, DE) ; MEYER; Christiane; (Meunster, DE) ; von FREYBURG; Martina; (Heibronn, DE) | ||||||||||
Applicant: |
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Assignee: | BOEHRINGER INGELHEIM VETMEDICA
GMBH Ingelheim DE |
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Family ID: | 35506051 | ||||||||||
Appl. No.: | 13/655278 | ||||||||||
Filed: | October 18, 2012 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
12463564 | May 11, 2009 | |||
13655278 | ||||
11132686 | May 19, 2005 | 7572455 | ||
12463564 | ||||
60589361 | Jul 20, 2004 | |||
Current U.S. Class: | 424/220.1 ; 435/236; 435/471; 536/23.72 |
Current CPC Class: | A61P 37/04 20180101; C12N 2770/24361 20130101; A61K 2039/5252 20130101; C12N 2770/24322 20130101; A61P 31/14 20180101; C07K 14/005 20130101; C12N 7/00 20130101 |
Class at Publication: | 424/220.1 ; 435/236; 435/471; 536/23.72 |
International Class: | C12N 7/04 20060101 C12N007/04; A61P 31/14 20060101 A61P031/14; C12N 15/40 20060101 C12N015/40; A61P 37/04 20060101 A61P037/04; A61K 39/187 20060101 A61K039/187; C12N 15/63 20060101 C12N015/63 |
Date | Code | Application Number |
---|---|---|
May 19, 2004 | DE | 10 2004 025 452 |
Sequence CWU 1
1
16112332DNAWildtyp BVDV XIKE-A 1gtatacgaga ttagctaaag aactcgtata
tggattggac gtcaacaaat ttttaattgg 60caacgtaggg aaccttcccc tcagcgaagg
ccgaaaagag gctagccatg cccttagtag 120gactagcaaa agtaggggac
tagcggtagc agtgagttcg ttggatggcc gaacccctga 180gtacagggga
gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg
240tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg
ggtgaaagcg 300ctattcgtgg cgttatggac acagcctgat agggtgtagc
agagacctgc tattccgcta 360gtaaaaactc tgctgtacat ggcacatgga
gttgttttca aatgaacttt tatacaaaac 420atataaacaa aaaccagcag
gcgtcgtgga acctgtttac gacgtcaacg ggcgcccact 480gtttggagag
agcagtgact tgcacccgca gtcaacacta aaactaccac accaacgagg
540cagcgccaac atcctgacca atgctaggtc cctaccgcgg aaaggtgact
gccggagagg 600taatgtgtat ggaccggtga gtggcatcta tatcaaacca
ggaccgatct actaccagga 660ttatgtgggc cccgtctatc atagagcccc
actggaacta tgtagggagg caagtatgtg 720cgaaacaact aggagagttg
gcagagtgac cggtagtgat gggaaattat atcatatcta 780catctgcata
gatgggtgta tcctcctgaa gagggcgact aggaaccaac cagaagtcct
840gaaatgggta tacaacagat taaattgtcc tttatgggtc accagctgct
ccgatgaagg 900gagcaagggt gctacaagta agaagcagcc taagccagat
aggatagaaa aaggtaagat 960gaaaatagcc ccaaaagaga cagaaaaaga
ttgcaaaacc agaccccccg acgcgactat 1020agtagtagaa ggggttaagt
accaggtgaa gaaaaaagga aaggtaaggg gaaaaaatac 1080tcaagatggg
ttatatcaca acaagaataa gccccctgaa tcaagaaaaa aattggaaaa
1140ggcactgctg gcttgggcca tcttagcagc ggtcctgctt cagctggtaa
caggagagaa 1200tatcacccag tggaacttga tggacaacgg caccgaggga
atacagcaag cgatgttcct 1260aagaggggtg aacaggagtc tacatggaat
ttggccagag aaaatttgca ccggagtacc 1320aactcactta gcaacagact
atgagcttaa agagatagtg gggatgatgg acgcgagtga 1380gaagaccaac
tacacgtgtt gcaggttgca aagacatgag tggaataaac atggttggtg
1440taactggttt catatagaac cgtggatatg gttgatgaac aaaacccaaa
acaacctgac 1500agaagggcaa ccgcttaggg agtgtgctgt gacttgtagg
tatgacaagg aaacagaatt 1560gaacatcgtg acacaggcta gggacagacc
tacaactctg acaggttgca agaaaggcaa 1620gaatttctct ttcgcaggtg
ttatactgga tgggccctgt aactttaaag tatcggttga 1680agatgtgctg
ttcaaggagc acgattgcgg caacatgctg caagagaccg cgatacagct
1740actcgatggg gcaaccaaca ccattgaggg agcaagggta gggacggcca
agttgacaac 1800ctggttaggg aagcaattag ggatccttgg taagaagttg
gagaacaaaa gcaaagcatg 1860gtttggtgca catgcagcaa gtccatactg
cggagtggag aggaagatcg gttacgtatg 1920gtatacaaaa aactgcactc
cagcttgcct tccaagaaac actagaataa taggccccgg 1980gaaatttgat
accaacgccg aagatggaaa aatactccat gagatggggg ggcacctctc
2040agaatttgtc ctattgtcct tggtggttct gtctgacttt gccccggaaa
ccgcgagcgt 2100catctacttg gttctacatt ttgcgatccc gcaaagccac
gttgatgtag acacatgcga 2160caagaaccag ctgaatttaa cggtagcaac
cacagtagca gaggtcatac cagggacagt 2220gtggaaccta gggaagtatg
tctgcataag accagactgg tggccatatg agacgacgac 2280agtcttcgtc
atagaggaag cagggcaagt aatcaaattg atgctaaggg ccatcagaga
2340cttaactagg atatggaatg ctgccactac cacagctttc ttaatctttt
tagtaaaagc 2400actgagggga caactaatcc aagggctatt gtggctgatg
ctaataacag gagcacaggg 2460cttccctgaa tgcaaagagg gcttccaata
tgccatatct aaagacagga aaatggggtt 2520attggggcca gagagcttaa
ctacaacatg gcacctcccc accaaaaaaa tagtggattc 2580catggtgcat
gtatggtgtg aaggaaaaga cttgaaaata ttaaaaatgt gcacaaagga
2640agagaggtat ctagtggctg tgcacgagag agccttatca accagtgccg
agtttatgca 2700gatcagtgat gggacaatag gcccagacgt gatagatatg
cctgatgact ttgagtttgg 2760actctgccct tgtgactcaa aaccagtgat
aaagggcaaa tttaatgcca gcttactgaa 2820tggaccagct ttccagatgg
tatgcccaca ggggtggact ggtacaatag aatgcaccct 2880agcgaaccaa
gacaccttgg acacaactgt cattaggaca tatagaagaa ctaccccatt
2940tcagcggaga aaatggtgta cctatgaaaa aataataggg gaagatatct
atgaatgcat 3000tctaggtgga aactggacat gcataaccgg tgaccatagc
aggttgaaag acggacctat 3060caagaagtgt aagtggtgtg gccatgactt
cgtcaactca gaggggctac cacactaccc 3120aataggcaag tgcatgctca
tcaacgagag tgggtacagg tatgtagatg acacctcttg 3180cgataggggt
ggtgtagcca tagttccatc tggcaccgta aagtgtagaa taggtaacgt
3240cacggtgcaa gttatcgcta ctaacaatga tctgggaccc atgccttgca
gcccagctga 3300agtgatagca agtgaaggac cagtggaaaa gactgcatgc
acattcaact attcaaggac 3360tctacctaat aagtattatg agccaaggga
ccggtacttc caacaataca tgttaaaagg 3420ggagtggcaa tattggttcg
acctggattc tgtagaccac cacaaagact acttctcaga 3480gttcataatc
atagcagtgg tcgccttgtt gggtggtaag tacgtactgt ggctcttgat
3540aacatacaca atactgtctg agcagatggc tatgggtgct ggagtgaata
ctgaagagat 3600agtcatgata ggcaatttgc tgacagacag tgatattgag
gttgtggttt atttccttct 3660tctgtactta atagttaaag aggaactggc
gaggaaatgg attatactgg tataccacat 3720ccttgtagcc aaccctatga
aaacaattgg ggtcgtctta ctaatgctag ggggagtggt 3780gaaggccagc
agaatcaatg ctgatgacca aagtgctatg gacccatgct ttcttctcgt
3840gacaggcgta gtggctgttt tgatgatcgc tagaagagaa cctgccacat
taccactgat 3900tgtagcattg ctagcaataa gaacatcagg attcctactg
cccgctagca ttgatgtaac 3960tgtagcagta gtattaattg tacttttgtt
ggctagctac ataacagact actttagata 4020taaaaagtgg cttcaactct
tatttagtct gatagctggt atctttatta taaggagctt 4080aaaacatatc
aaccagatgg aggtaccaga aatatctatg ccaagttgga gacctctagc
4140tctggtcctt ttctatataa catctacagc aataaccact aattgggaca
ttgacttagc 4200aggcttcctg ctgcaatggg cgccagcagt gatcatgatg
gctaccatgt gggcagactt 4260tttgactctg atcatagtcc tgcccagtta
cgagttatct aagctttact tcctaaagaa 4320cgtcaggaca gacgtggaaa
agaactggct cggcaaagtg aaatacagac agatcagttc 4380agtttatgac
atctgtgaca gtgaggaagc agtgtaccta tttccatcaa ggcataagag
4440tggaagcagg ccagatttca tattaccttt tttgaaagcc gtgttaataa
gctgcatcag 4500cagccaatgg caagtggttt acatttctta cctaatactg
gaaattacat actatatgca 4560caggaaaatc atagatgagg tgtcaggagg
agcaaatttt ctatcaagac tcatagcagc 4620catcatagaa ttaaattggg
ccatagatga tgaggaatgt aaaggactga agaaactgta 4680tctcttgtca
gggagagcga agaatttgat agttaaacat aaggtaagaa atgaagccgt
4740ccacagatgg tttggtgagg aggaaatata cggggcaccc aaggtgatca
ctatcataaa 4800agctagtacc ctaagtaaaa acaggcactg cataatctgc
acgatctgtg aagggaaaga 4860atggaatgga gccaactgcc caaagtgtgg
aagacaagga aagcccataa catgtggaat 4920gacactcgca gactttgagg
agaaacatta caaaaagata tttataagag aagaatcttc 4980ttgtcctgtg
ccttttgatc cttcttgcca ttgtaattat tttcgccacg atgggccttt
5040caggaaagag tataagggtt acgtccaata cacagccaga ggacaactct
ttctgaggaa 5100cctaccaatt ctagcgacga agatgaagct attaatggtg
ggaaacctcg gcgcagaaat 5160tggcgacctg gaacatctag gatgggtact
gagagggcca gccgtgtgca aaaaaattac 5220caaccatgag aagtgccacg
taaacatcat ggataagcta actgcatttt ttggaatcat 5280gcctagaggc
acgaccccta gggcacctgt gaggttcccc acagcactac taaaagtgag
5340aagggggcta gagacgggat gggcttacac gcaccaagga gggatcagct
cggtagacca 5400tgtcacagcc ggaaaggatt tactagtgtg tgacagtatg
ggcaggacca gggttgtctg 5460tcatagtaac aataagatga ctgatgagac
tgagtatggc atcaagaccg actcagggtg 5520tcccgaaggt gcgaggtgtt
acgtgctaaa cccagaagct gttaacattt ctggcacaaa 5580aggagctatg
gtacacctcc agaaaacggg gggggagttc acatgtgtca ctgcctcagg
5640gaccccggct ttcttcgatc tgaaaaatct aaaaggctgg tccgggctac
caatttttga 5700agcatccagt ggcagggtgg ttggtagggt gaaagtcggc
aagaatgagg attccaagcc 5760caccaaacta atgagcggaa tccagacagt
gtctaagaac cagacagacc tagcggacat 5820cgtaaaaaaa ttgactagta
tgaacagagg agagttcaaa cagataacat tagccactgg 5880ggcaggaaaa
actacggaac tgccaaggtc cgtcatagag gagataggga ggcacaaaag
5940ggtcttagtc ctgataccat tgagagcagc agcagagtca gtgtatcagt
atatgagagt 6000gaagtaccca agtatatctt tcaatttgag aataggagat
atgaaggaag gtgacatggc 6060cactggtatc acctacgcct catatgggta
cttttgtcag cttcctcagc ccaaactgag 6120agctgccatg gtagagtact
catatatatt cttagatgag taccactgtg ctacacccga 6180gcaattagca
ataattggaa agatacacag gtttgctgaa aatcttagag tggtagcaat
6240gacagcaacc ccagctggaa cggtcacaac gactggtcag aaacacccta
tagaggagtt 6300catagcccca gaggtgatga aaggtgaaga tctaggtagt
gaatacttgg atattgcagg 6360gttgaagata ccgactgaag agatgaaagg
caacatgctc gtgttcgcgc caactaggaa 6420catggcagta gaaacagcta
agaaattgaa ggctaaggga tacaactctg gatactatta 6480cagtggggaa
aacccagaga acttgagggt ggtaacctcg caatccccgt atgtggtagt
6540agccaccaat gccatagagt caggtgtgac attaccagac ttagacacag
ttgtagacac 6600tggactaaag tgtgagaaga gggtgaggat ttcttcaaaa
atgcccttca ttgtaacagg 6660acttaagaga atggcagtca caatcggaga
gcaagcccag cgcaggggta gagtaggaag 6720agtcaagcca ggtaggtact
ataggagtca agaaacagct tcagggtcaa aagattacca 6780ttacgaccta
ctgcaagccc agaggtacgg aatagaagat ggaattaatg taacaaagtc
6840attcagggag atgaactatg attggagcct ttacgaagag gacagcttga
tgataactca 6900actcgaggtc cttaacaacc tccttatatc agaagacctg
cctgccgcag tgaagaacat 6960catggcccgg accgatcacc cagaacccat
acaactggcc tataacagtt atgaaaacca 7020aattccagtg ctgttcccaa
agatcaaaaa tggtgaggtg acagacagtt atgagaatta 7080cacatatctc
aatgcaagaa aattaggaga ggacgtgccg gcatatgtgt acgccacaga
7140ggatgaggat ctagcagtgg atcttctggg tatggattgg ccggacccag
gcaaccaaca 7200ggtggtagag acagggaggg cattaaaaca agtaactggc
ttatccacag cagaaaacgc 7260cctcttgata gccctattcg gctacgtcgg
gtaccagaca ctttcaaaaa ggcacatacc 7320catgattact gacatctata
cacttgaaga ccacaggctt gaggacacaa cccacctcca 7380gtttgcccca
aacgctataa ggaccgacgg caaggactca gagttgaagg aattagctgt
7440gggagacctt gataaatatg tggacgcact ggtagactac tccaaacaag
ggatgaaatt 7500catcaaagtc caagctgaaa aggtcagaga ctcccagtct
acgaaggaag gcttgcaaac 7560cattaaggag tatgtggata agtttataca
atcactaaca gagaataagg aggagatcat 7620caggtatgga ctatggggag
ttcacacggc actctacaaa agcttggcag cgagactggg 7680gcatgaaaca
gcttttgcaa ctttagtggt aaaatggttg gcttttgggg gcgaaacggt
7740atctgctcac atcaagcaag tagcagttga tctagtagta tattatatca
tcaacaaacc 7800atcttttcct ggagatacag agacccaaca agaggggagg
aagtttgtgg ctagtctttt 7860tatatctgca ctagcaacat acacatataa
aacctggaat tacaacaatc tgcaacgggt 7920tgtcgaacct gccttagctt
acctcccata tgctacaagt gccttgaagt tgttcacacc 7980cacaagatta
gagagtgtgg tcatactcag ttctacaatt tacaagacat acctctctat
8040aaggaagggt aagagtgacg gcttgttagg tacaggcata agtgcagcca
tggagatctt 8100aaaccaaaac ccaatctcag taggtatatc tgtgatgctg
ggggtaggtg ccatcgccgc 8160ccataatgca atagaatcta gtgaacagaa
aagaactttg ctgatgaagg tctttgtaaa 8220aaacttctta gaccaagcag
caacagatga gctagtcaaa gagaaccctg aaaaaataat 8280catggctcta
tttgaagcag tccagaccat aggaaacccc ctaagactca tctaccatct
8340gtacggggtg tactataagg ggtgggaagc aaaagaactc gcagagaaaa
ctgctggccg 8400caacttattc acattgatca tgtttgaggc ctttgagctt
ttaggtatgg actcagaagg 8460aaagataaga aacttgtcag gcaactacat
actggactta atcttcaact tgcataataa 8520attaaacaag gggctcaaaa
aactagtcct tgggtgggct cctgcacctt tgagctgtga 8580ttggacacca
agtgatgaga gaataagcct acctcataac aactacttaa gggtagaaac
8640caggtgtcct tgtggctatg agatgaaggc aataaaaaat gttgctggta
aattgacaaa 8700agttgaagaa aaggggtcct tcctatgcag gaatagatta
gggagaggac ctccaaactt 8760caaagtaaca aagttctatg atgataactt
gatagaagtc aagccagtag ctaggctaga 8820aggccaggtg gacctctatt
acaagggagt aacagctaag ttagactaca acaatgggaa 8880agtactgtta
gctaccaaca agtgggaggt ggaccacgct ttcctgacca gactagtaaa
8940gaagcacaca gggataggtt ttaaaggtgc atatttgggt gaccgaccag
accatcaaga 9000tcttgtcgat agagattgtg caactataac gaagaactca
gtacagttcc taaaaatgaa 9060gaagggttgc gctttcacat atgacctaac
aatctctaac cttgtcaggc ttattgaact 9120agtccataag aataatttac
aagaaagaga gatccctacc gtgacagtaa ctacttggct 9180tgcatattct
tttgtcaatg aagacctggg gactatcaag cctgtattgg gggagaaagt
9240catcccagaa ccccccgagg agttgagtct ccaacccacc gtgagactag
tcaccactga 9300aacagcaata accataacag gggaggctga agtgatgacg
acagggatca caccagtggt 9360agagatgaaa gaagaacctc agctggacca
ccagtcaact accctaaagg tagggttgaa 9420ggaaggggaa tatccagggc
caggagttaa ccctaaccat ttagcagagg tgatagatga 9480gaaagatgac
aggccttttg tcctaatcat cggtaacaaa ggttctacct cgaacagagc
9540aagaacggcc aagaatatac ggctgtacaa aggaaacaac ccaagagaga
tcagggatct 9600gatgagccaa ggaagaatat tgacggttgc tctaaaagag
ttggacccgg aattaaaaga 9660attagtagat tacaagggga cctttctcaa
tagggaagct ttagaagccc taagcttagg 9720taagccaatc aagaggaaaa
ccacaacagc aatgatcagg aggttaatag agccagaggt 9780tgaggaggaa
ctaccagatt ggttccaagc ggaagaaccc ctatttttgg aagcaaaaat
9840acagaatgac ttataccacc taattggcag tgtagatagt ataaaaagca
aagcaaagga 9900attaggggcc acagataaca caaagatagt gaaggaagtt
ggggctagga cctatacgat 9960gaaattgagc agctggagca cacaagttac
aaaaaaacag atgagtctag cccctctctt 10020tgaagagctg ttattaaagt
gccctccatg tagtaaaatt tcaaagggac atatggtgtc 10080agcataccaa
ctggctcaag gaaactggga acccctcggg tgtggggtct atatgggaac
10140cataccagct aggcgtctca agatccaccc ttatgaggct taccttaaac
tcaaagagct 10200ggtggaagtt gaatcttcga gggccactgc aaaagaatcc
atcataagag aacataacac 10260ctggatcctg cggaaggtga gacatgaagg
gaacctaaga accaaatcaa tgatcaaccc 10320tgggaaaata tcagatcagc
tatgcagaga tggacacaaa agaaacatat ataataagat 10380cataggctca
acaatggcct ctgctggtat taggctggag aaactgccag tagtccgagc
10440ccaaactgac acaaccagtt tccaccaagc cataagagaa aaaattgata
aaacagaaaa 10500caagcagacc cctgaattgc atgaagaact aatgaaggtc
ttcgactgct taaagatccc 10560agagctgaag gaatcgtatg atgaagtttc
atgggaacaa ttagaagccg ggataaaccg 10620taagggtgca gcaggctatc
tagagagcaa gaacataggg gaagtcctag acacagagaa 10680acacatagta
gagcagctga tcaaggatct gaggaagggg aagaagatta ggtactatga
10740aacagccatc cccaagaatg agaagagaga cgtcagcgac gactgggaag
ccggagagtt 10800cgttgatgaa aagaaaccaa gagtaatcca gtacccggac
gccaaggtga gactggccat 10860tacaaaagtg atgtacaaat gggtaaagca
aaaaccagtg gtgatacccg gctatgaagg 10920taaaacacct ctatttgaca
tattcaacaa agtgaagaag gaatgggatt cattccagga 10980ccccgtagca
gtgagctttg acaccaaagc gtgggataca caagtcacca gtagagacct
11040aatgttgata aaggatatcc agaaatatta tttcaagaga agtatacaca
aatttttaga 11100tacaataaca gaacacatgg tggaggtacc tgtcattaca
gcagacggtg aagtttacat 11160aaggaatggt cagaggggta gtggccaacc
cgacacaagt gctggtaata gtatgttgaa 11220tgtcctaacc atgatatatg
ctttctgtaa aagtacaggc ataccttaca ggggattcag 11280cagagtggca
agaatccatg tgtgtggtga tgatggcttt ttgataacag agagaggact
11340gggactgaaa ttctctgaga agggtatgca gatattacat gaggccggga
agccccagaa 11400aataactgaa ggggacaaaa tgaaagtggc atacagattc
gaggacatag agttttgttc 11460ccatactccc gtgccagtca gatgggcaga
taacaccagt agttacatgg cagggaggag 11520cacagccact atactagcta
agatggcaac caggctggat tccagcggag agaggggtag 11580cacagcttat
gagaaggccg tagccttcag cttccttttg atgtactcat ggaatcccgt
11640agttagaagg atctgcttac tggtgttgtc acagtttcca gaaatatccc
catccaaaaa 11700cacaatatac tactaccaag gggatcccat agctgcgtac
agagaagtga tagggaaaca 11760gctgtgtgaa ctgaaaagaa caggatttga
gaagctggct ggtctgaatt tgagtatgac 11820cactctaggc atctggacaa
aacatactag taaaagacta atccaagcct gtgtagaaat 11880aggtaagaga
gaaggtacct ggttagttaa tgctgacaga ctgattgcag gaaagactgg
11940gaagttttac atcccaagca ctggtgtcac tctgttggga aaacactatg
aggaaattaa 12000cttaaagcaa aaggcggcac aaccgccgat agagggggtt
gacagatata agttgggccc 12060catagttaat gttatcttga gaaggctgag
ggtgatgctg atgacagttg ccagcggaag 12120ctggtgaatc cgtccggagc
gtcgtgccct cactcaaggt ttttaattgt aaatattgta 12180aatagacagc
taagatattt attgtagttg gatagtaatg cagtgatagt aaatacccca
12240atttaacact acctccaatg cactaagcac tttagctgtg tgaggttaac
tcgacgtcca 12300cggttggact agggaagacc tctaacagcc cc
12332211840DNAArtificial SequenceMutated BVDV XIKE- A-NdN
2gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg
60caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag
120gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc
gaacccctga 180gtacagggga gtcgtcaatg gttcgacact ccattagtcg
aggagtctcg agatgccatg 240tggacgaggg catgcccacg gcacatctta
acccatgcgg gggttgcatg ggtgaaagcg 300ctattcgtgg cgttatggac
acagcctgat agggtgtagc agagacctgc tattccgcta 360gtaaaaactc
tgctgtacat ggcacatgga gttgttttcc gatgaaggga gcaagggtgc
420tacaagtaag aagcagccta agccagatag gatagaaaaa ggtaagatga
aaatagcccc 480aaaagagaca gaaaaagatt gcaaaaccag accccccgac
gcgactatag tagtagaagg 540ggttaagtac caggtgaaga aaaaaggaaa
ggtaagggga aaaaatactc aagatgggtt 600atatcacaac aagaataagc
cccctgaatc aagaaaaaaa ttggaaaagg cactgctggc 660ttgggccatc
ttagcagcgg tcctgcttca gctggtaaca ggagagaata tcacccagtg
720gaacttgatg gacaacggca ccgagggaat acagcaagcg atgttcctaa
gaggggtgaa 780caggagtcta catggaattt ggccagagaa aatttgcacc
ggagtaccaa ctcacttagc 840aacagactat gagcttaaag agatagtggg
gatgatggac gcgagtgaga agaccaacta 900cacgtgttgc aggttgcaaa
gacatgagtg gaataaacat ggttggtgta actggtttca 960tatagaaccg
tggatatggt tgatgaacaa aacccaaaac aacctgacag aagggcaacc
1020gcttagggag tgtgctgtga cttgtaggta tgacaaggaa acagaattga
acatcgtgac 1080acaggctagg gacagaccta caactctgac aggttgcaag
aaaggcaaga atttctcttt 1140cgcaggtgtt atactggatg ggccctgtaa
ctttaaagta tcggttgaag atgtgctgtt 1200caaggagcac gattgcggca
acatgctgca agagaccgcg atacagctac tcgatggggc 1260aaccaacacc
attgagggag caagggtagg gacggccaag ttgacaacct ggttagggaa
1320gcaattaggg atccttggta agaagttgga gaacaaaagc aaagcatggt
ttggtgcaca 1380tgcagcaagt ccatactgcg gagtggagag gaagatcggt
tacgtatggt atacaaaaaa 1440ctgcactcca gcttgccttc caagaaacac
tagaataata ggccccggga aatttgatac 1500caacgccgaa gatggaaaaa
tactccatga gatggggggg cacctctcag aatttgtcct 1560attgtccttg
gtggttctgt ctgactttgc cccggaaacc gcgagcgtca tctacttggt
1620tctacatttt gcgatcccgc aaagccacgt tgatgtagac acatgcgaca
agaaccagct 1680gaatttaacg gtagcaacca cagtagcaga ggtcatacca
gggacagtgt ggaacctagg 1740gaagtatgtc tgcataagac cagactggtg
gccatatgag acgacgacag tcttcgtcat 1800agaggaagca gggcaagtaa
tcaaattgat gctaagggcc atcagagact taactaggat 1860atggaatgct
gccactacca cagctttctt aatcttttta gtaaaagcac tgaggggaca
1920actaatccaa gggctattgt ggctgatgct aataacagga gcacagggct
tccctgaatg 1980caaagagggc ttccaatatg ccatatctaa agacaggaaa
atggggttat tggggccaga 2040gagcttaact acaacatggc acctccccac
caaaaaaata gtggattcca tggtgcatgt 2100atggtgtgaa ggaaaagact
tgaaaatatt aaaaatgtgc acaaaggaag agaggtatct 2160agtggctgtg
cacgagagag ccttatcaac cagtgccgag tttatgcaga tcagtgatgg
2220gacaataggc ccagacgtga tagatatgcc tgatgacttt gagtttggac
tctgcccttg 2280tgactcaaaa ccagtgataa agggcaaatt taatgccagc
ttactgaatg gaccagcttt 2340ccagatggta tgcccacagg ggtggactgg
tacaatagaa tgcaccctag cgaaccaaga 2400caccttggac acaactgtca
ttaggacata tagaagaact accccatttc agcggagaaa 2460atggtgtacc
tatgaaaaaa taatagggga agatatctat gaatgcattc taggtggaaa
2520ctggacatgc ataaccggtg accatagcag gttgaaagac ggacctatca
agaagtgtaa 2580gtggtgtggc catgacttcg tcaactcaga
ggggctacca cactacccaa taggcaagtg 2640catgctcatc aacgagagtg
ggtacaggta tgtagatgac acctcttgcg ataggggtgg 2700tgtagccata
gttccatctg gcaccgtaaa gtgtagaata ggtaacgtca cggtgcaagt
2760tatcgctact aacaatgatc tgggacccat gccttgcagc ccagctgaag
tgatagcaag 2820tgaaggacca gtggaaaaga ctgcatgcac attcaactat
tcaaggactc tacctaataa 2880gtattatgag ccaagggacc ggtacttcca
acaatacatg ttaaaagggg agtggcaata 2940ttggttcgac ctggattctg
tagaccacca caaagactac ttctcagagt tcataatcat 3000agcagtggtc
gccttgttgg gtggtaagta cgtactgtgg ctcttgataa catacacaat
3060actgtctgag cagatggcta tgggtgctgg agtgaatact gaagagatag
tcatgatagg 3120caatttgctg acagacagtg atattgaggt tgtggtttat
ttccttcttc tgtacttaat 3180agttaaagag gaactggcga ggaaatggat
tatactggta taccacatcc ttgtagccaa 3240ccctatgaaa acaattgggg
tcgtcttact aatgctaggg ggagtggtga aggccagcag 3300aatcaatgct
gatgaccaaa gtgctatgga cccatgcttt cttctcgtga caggcgtagt
3360ggctgttttg atgatcgcta gaagagaacc tgccacatta ccactgattg
tagcattgct 3420agcaataaga acatcaggat tcctactgcc cgctagcatt
gatgtaactg tagcagtagt 3480attaattgta cttttgttgg ctagctacat
aacagactac tttagatata aaaagtggct 3540tcaactctta tttagtctga
tagctggtat ctttattata aggagcttaa aacatatcaa 3600ccagatggag
gtaccagaaa tatctatgcc aagttggaga cctctagctc tggtcctttt
3660ctatataaca tctacagcaa taaccactaa ttgggacatt gacttagcag
gcttcctgct 3720gcaatgggcg ccagcagtga tcatgatggc taccatgtgg
gcagactttt tgactctgat 3780catagtcctg cccagttacg agttatctaa
gctttacttc ctaaagaacg tcaggacaga 3840cgtggaaaag aactggctcg
gcaaagtgaa atacagacag atcagttcag tttatgacat 3900ctgtgacagt
gaggaagcag tgtacctatt tccatcaagg cataagagtg gaagcaggcc
3960agatttcata ttaccttttt tgaaagccgt gttaataagc tgcatcagca
gccaatggca 4020agtggtttac atttcttacc taatactgga aattacatac
tatatgcaca ggaaaatcat 4080agatgaggtg tcaggaggag caaattttct
atcaagactc atagcagcca tcatagaatt 4140aaattgggcc atagatgatg
aggaatgtaa aggactgaag aaactgtatc tcttgtcagg 4200gagagcgaag
aatttgatag ttaaacataa ggtaagaaat gaagccgtcc acagatggtt
4260tggtgaggag gaaatatacg gggcacccaa ggtgatcact atcataaaag
ctagtaccct 4320aagtaaaaac aggcactgca taatctgcac gatctgtgaa
gggaaagaat ggaatggagc 4380caactgccca aagtgtggaa gacaaggaaa
gcccataaca tgtggaatga cactcgcaga 4440ctttgaggag aaacattaca
aaaagatatt tataagagaa gaatcttctt gtcctgtgcc 4500ttttgatcct
tcttgccatt gtaattattt tcgccacgat gggcctttca ggaaagagta
4560taagggttac gtccaataca cagccagagg acaactcttt ctgaggaacc
taccaattct 4620agcgacgaag atgaagctat taatggtggg aaacctcggc
gcagaaattg gcgacctgga 4680acatctagga tgggtactga gagggccagc
cgtgtgcaaa aaaattacca accatgagaa 4740gtgccacgta aacatcatgg
ataagctaac tgcatttttt ggaatcatgc ctagaggcac 4800gacccctagg
gcacctgtga ggttccccac agcactacta aaagtgagaa gggggctaga
4860gacgggatgg gcttacacgc accaaggagg gatcagctcg gtagaccatg
tcacagccgg 4920aaaggattta ctagtgtgtg acagtatggg caggaccagg
gttgtctgtc atagtaacaa 4980taagatgact gatgagactg agtatggcat
caagaccgac tcagggtgtc ccgaaggtgc 5040gaggtgttac gtgctaaacc
cagaagctgt taacatttct ggcacaaaag gagctatggt 5100acacctccag
aaaacggggg gggagttcac atgtgtcact gcctcaggga ccccggcttt
5160cttcgatctg aaaaatctaa aaggctggtc cgggctacca atttttgaag
catccagtgg 5220cagggtggtt ggtagggtga aagtcggcaa gaatgaggat
tccaagccca ccaaactaat 5280gagcggaatc cagacagtgt ctaagaacca
gacagaccta gcggacatcg taaaaaaatt 5340gactagtatg aacagaggag
agttcaaaca gataacatta gccactgggg caggaaaaac 5400tacggaactg
ccaaggtccg tcatagagga gatagggagg cacaaaaggg tcttagtcct
5460gataccattg agagcagcag cagagtcagt gtatcagtat atgagagtga
agtacccaag 5520tatatctttc aatttgagaa taggagatat gaaggaaggt
gacatggcca ctggtatcac 5580ctacgcctca tatgggtact tttgtcagct
tcctcagccc aaactgagag ctgccatggt 5640agagtactca tatatattct
tagatgagta ccactgtgct acacccgagc aattagcaat 5700aattggaaag
atacacaggt ttgctgaaaa tcttagagtg gtagcaatga cagcaacccc
5760agctggaacg gtcacaacga ctggtcagaa acaccctata gaggagttca
tagccccaga 5820ggtgatgaaa ggtgaagatc taggtagtga atacttggat
attgcagggt tgaagatacc 5880gactgaagag atgaaaggca acatgctcgt
gttcgcgcca actaggaaca tggcagtaga 5940aacagctaag aaattgaagg
ctaagggata caactctgga tactattaca gtggggaaaa 6000cccagagaac
ttgagggtgg taacctcgca atccccgtat gtggtagtag ccaccaatgc
6060catagagtca ggtgtgacat taccagactt agacacagtt gtagacactg
gactaaagtg 6120tgagaagagg gtgaggattt cttcaaaaat gcccttcatt
gtaacaggac ttaagagaat 6180ggcagtcaca atcggagagc aagcccagcg
caggggtaga gtaggaagag tcaagccagg 6240taggtactat aggagtcaag
aaacagcttc agggtcaaaa gattaccatt acgacctact 6300gcaagcccag
aggtacggaa tagaagatgg aattaatgta acaaagtcat tcagggagat
6360gaactatgat tggagccttt acgaagagga cagcttgatg ataactcaac
tcgaggtcct 6420taacaacctc cttatatcag aagacctgcc tgccgcagtg
aagaacatca tggcccggac 6480cgatcaccca gaacccatac aactggccta
taacagttat gaaaaccaaa ttccagtgct 6540gttcccaaag atcaaaaatg
gtgaggtgac agacagttat gagaattaca catatctcaa 6600tgcaagaaaa
ttaggagagg acgtgccggc atatgtgtac gccacagagg atgaggatct
6660agcagtggat cttctgggta tggattggcc ggacccaggc aaccaacagg
tggtagagac 6720agggagggca ttaaaacaag taactggctt atccacagca
gaaaacgccc tcttgatagc 6780cctattcggc tacgtcgggt accagacact
ttcaaaaagg cacataccca tgattactga 6840catctataca cttgaagacc
acaggcttga ggacacaacc cacctccagt ttgccccaaa 6900cgctataagg
accgacggca aggactcaga gttgaaggaa ttagctgtgg gagaccttga
6960taaatatgtg gacgcactgg tagactactc caaacaaggg atgaaattca
tcaaagtcca 7020agctgaaaag gtcagagact cccagtctac gaaggaaggc
ttgcaaacca ttaaggagta 7080tgtggataag tttatacaat cactaacaga
gaataaggag gagatcatca ggtatggact 7140atggggagtt cacacggcac
tctacaaaag cttggcagcg agactggggc atgaaacagc 7200ttttgcaact
ttagtggtaa aatggttggc ttttgggggc gaaacggtat ctgctcacat
7260caagcaagta gcagttgatc tagtagtata ttatatcatc aacaaaccat
cttttcctgg 7320agatacagag acccaacaag aggggaggaa gtttgtggct
agtcttttta tatctgcact 7380agcaacatac acatataaaa cctggaatta
caacaatctg caacgggttg tcgaacctgc 7440cttagcttac ctcccatatg
ctacaagtgc cttgaagttg ttcacaccca caagattaga 7500gagtgtggtc
atactcagtt ctacaattta caagacatac ctctctataa ggaagggtaa
7560gagtgacggc ttgttaggta caggcataag tgcagccatg gagatcttaa
accaaaaccc 7620aatctcagta ggtatatctg tgatgctggg ggtaggtgcc
atcgccgccc ataatgcaat 7680agaatctagt gaacagaaaa gaactttgct
gatgaaggtc tttgtaaaaa acttcttaga 7740ccaagcagca acagatgagc
tagtcaaaga gaaccctgaa aaaataatca tggctctatt 7800tgaagcagtc
cagaccatag gaaaccccct aagactcatc taccatctgt acggggtgta
7860ctataagggg tgggaagcaa aagaactcgc agagaaaact gctggccgca
acttattcac 7920attgatcatg tttgaggcct ttgagctttt aggtatggac
tcagaaggaa agataagaaa 7980cttgtcaggc aactacatac tggacttaat
cttcaacttg cataataaat taaacaaggg 8040gctcaaaaaa ctagtccttg
ggtgggctcc tgcacctttg agctgtgatt ggacaccaag 8100tgatgagaga
ataagcctac ctcataacaa ctacttaagg gtagaaacca ggtgtccttg
8160tggctatgag atgaaggcaa taaaaaatgt tgctggtaaa ttgacaaaag
ttgaagaaaa 8220ggggtccttc ctatgcagga atagattagg gagaggacct
ccaaacttca aagtaacaaa 8280gttctatgat gataacttga tagaagtcaa
gccagtagct aggctagaag gccaggtgga 8340cctctattac aagggagtaa
cagctaagtt agactacaac aatgggaaag tactgttagc 8400taccaacaag
tgggaggtgg accacgcttt cctgaccaga ctagtaaaga agcacacagg
8460gataggtttt aaaggtgcat atttgggtga ccgaccagac catcaagatc
ttgtcgatag 8520agattgtgca actataacga agaactcagt acagttccta
aaaatgaaga agggttgcgc 8580tttcacatat gacctaacaa tctctaacct
tgtcaggctt attgaactag tccataagaa 8640taatttacaa gaaagagaga
tccctaccgt gacagtaact acttggcttg catattcttt 8700tgtcaatgaa
gacctgggga ctatcaagcc tgtattgggg gagaaagtca tcccagaacc
8760ccccgaggag ttgagtctcc aacccaccgt gagactagtc accactgaaa
cagcaataac 8820cataacaggg gaggctgaag tgatgacgac agggatcaca
ccagtggtag agatgaaaga 8880agaacctcag ctggaccacc agtcaactac
cctaaaggta gggttgaagg aaggggaata 8940tccagggcca ggagttaacc
ctaaccattt agcagaggtg atagatgaga aagatgacag 9000gccttttgtc
ctaatcatcg gtaacaaagg ttctacctcg aacagagcaa gaacggccaa
9060gaatatacgg ctgtacaaag gaaacaaccc aagagagatc agggatctga
tgagccaagg 9120aagaatattg acggttgctc taaaagagtt ggacccggaa
ttaaaagaat tagtagatta 9180caaggggacc tttctcaata gggaagcttt
agaagcccta agcttaggta agccaatcaa 9240gaggaaaacc acaacagcaa
tgatcaggag gttaatagag ccagaggttg aggaggaact 9300accagattgg
ttccaagcgg aagaacccct atttttggaa gcaaaaatac agaatgactt
9360ataccaccta attggcagtg tagatagtat aaaaagcaaa gcaaaggaat
taggggccac 9420agataacaca aagatagtga aggaagttgg ggctaggacc
tatacgatga aattgagcag 9480ctggagcaca caagttacaa aaaaacagat
gagtctagcc cctctctttg aagagctgtt 9540attaaagtgc cctccatgta
gtaaaatttc aaagggacat atggtgtcag cataccaact 9600ggctcaagga
aactgggaac ccctcgggtg tggggtctat atgggaacca taccagctag
9660gcgtctcaag atccaccctt atgaggctta ccttaaactc aaagagctgg
tggaagttga 9720atcttcgagg gccactgcaa aagaatccat cataagagaa
cataacacct ggatcctgcg 9780gaaggtgaga catgaaggga acctaagaac
caaatcaatg atcaaccctg ggaaaatatc 9840agatcagcta tgcagagatg
gacacaaaag aaacatatat aataagatca taggctcaac 9900aatggcctct
gctggtatta ggctggagaa actgccagta gtccgagccc aaactgacac
9960aaccagtttc caccaagcca taagagaaaa aattgataaa acagaaaaca
agcagacccc 10020tgaattgcat gaagaactaa tgaaggtctt cgactgctta
aagatcccag agctgaagga 10080atcgtatgat gaagtttcat gggaacaatt
agaagccggg ataaaccgta agggtgcagc 10140aggctatcta gagagcaaga
acatagggga agtcctagac acagagaaac acatagtaga 10200gcagctgatc
aaggatctga ggaaggggaa gaagattagg tactatgaaa cagccatccc
10260caagaatgag aagagagacg tcagcgacga ctgggaagcc ggagagttcg
ttgatgaaaa 10320gaaaccaaga gtaatccagt acccggacgc caaggtgaga
ctggccatta caaaagtgat 10380gtacaaatgg gtaaagcaaa aaccagtggt
gatacccggc tatgaaggta aaacacctct 10440atttgacata ttcaacaaag
tgaagaagga atgggattca ttccaggacc ccgtagcagt 10500gagctttgac
accaaagcgt gggatacaca agtcaccagt agagacctaa tgttgataaa
10560ggatatccag aaatattatt tcaagagaag tatacacaaa tttttagata
caataacaga 10620acacatggtg gaggtacctg tcattacagc agacggtgaa
gtttacataa ggaatggtca 10680gaggggtagt ggccaacccg acacaagtgc
tggtaatagt atgttgaatg tcctaaccat 10740gatatatgct ttctgtaaaa
gtacaggcat accttacagg ggattcagca gagtggcaag 10800aatccatgtg
tgtggtgatg atggcttttt gataacagag agaggactgg gactgaaatt
10860ctctgagaag ggtatgcaga tattacatga ggccgggaag ccccagaaaa
taactgaagg 10920ggacaaaatg aaagtggcat acagattcga ggacatagag
ttttgttccc atactcccgt 10980gccagtcaga tgggcagata acaccagtag
ttacatggca gggaggagca cagccactat 11040actagctaag atggcaacca
ggctggattc cagcggagag aggggtagca cagcttatga 11100gaaggccgta
gccttcagct tccttttgat gtactcatgg aatcccgtag ttagaaggat
11160ctgcttactg gtgttgtcac agtttccaga aatatcccca tccaaaaaca
caatatacta 11220ctaccaaggg gatcccatag ctgcgtacag agaagtgata
gggaaacagc tgtgtgaact 11280gaaaagaaca ggatttgaga agctggctgg
tctgaatttg agtatgacca ctctaggcat 11340ctggacaaaa catactagta
aaagactaat ccaagcctgt gtagaaatag gtaagagaga 11400aggtacctgg
ttagttaatg ctgacagact gattgcagga aagactggga agttttacat
11460cccaagcact ggtgtcactc tgttgggaaa acactatgag gaaattaact
taaagcaaaa 11520ggcggcacaa ccgccgatag agggggttga cagatataag
ttgggcccca tagttaatgt 11580tatcttgaga aggctgaggg tgatgctgat
gacagttgcc agcggaagct ggtgaatccg 11640tccggagcgt cgtgccctca
ctcaaggttt ttaattgtaa atattgtaaa tagacagcta 11700agatatttat
tgtagttgga tagtaatgca gtgatagtaa ataccccaat ttaacactac
11760ctccaatgca ctaagcactt tagctgtgtg aggttaactc gacgtccacg
gttggactag 11820ggaagacctc taacagcccc 11840312329DNAArtificial
SequenceMutated BVDV XIKE-B 3gtatacgaga ttagctaaag aactcgtata
tggattggac gtcaacaaat ttttaattgg 60caacgtaggg aaccttcccc tcagcgaagg
ccgaaaagag gctagccatg cccttagtag 120gactagcaaa agtaggggac
tagcggtagc agtgagttcg ttggatggcc gaacccctga 180gtacagggga
gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg
240tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg
ggtgaaagcg 300ctattcgtgg cgttatggac acagcctgat agggtgtagc
agagacctgc tattccgcta 360gtaaaaactc tgctgtacat ggcacatgga
gttgttttca aatgaacttt tatacaaaac 420atataaacaa aaaccagcag
gcgtcgtgga acctgtttac gacgtcaacg ggcgcccact 480gtttggagag
agcagtgact tgcacccgca gtcaacacta aaactaccac accaacgagg
540cagcgccaac atcctgacca atgctaggtc cctaccgcgg aaaggtgact
gccggagagg 600taatgtgtat ggaccggtga gtggcatcta tatcaaacca
ggaccgatct actaccagga 660ttatgtgggc cccgtctatc atagagcccc
actggaacta tgtagggagg caagtatgtg 720cgaaacaact aggagagttg
gcagagtgac cggtagtgat gggaaattat atcatatcta 780catctgcata
gatgggtgta tcctcctgaa gagggcgact aggaaccaac cagaagtcct
840gaaatgggta tacaacagat taaattgtcc tttatgggtc accagctgct
ccgatgaagg 900gagcaagggt gctacaagta agaagcagcc taagccagat
aggatagaaa aaggtaagat 960gaaaatagcc ccaaaagaga cagaaaaaga
ttgcaaaacc agaccccccg acgcgactat 1020agtagtagaa ggggttaagt
accaggtgaa gaaaaaagga aaggtaaggg gaaaaaatac 1080tcaagatggg
ttatatcaca acaagaataa gccccctgaa tcaagaaaaa aattggaaaa
1140ggcactgctg gcttgggcca tcttagcagc ggtcctgctt cagctggtaa
caggagagaa 1200tatcacccag tggaacttga tggacaacgg caccgaggga
atacagcaag cgatgttcct 1260aagaggggtg aacaggagtc tacatggaat
ttggccagag aaaatttgca ccggagtacc 1320aactcactta gcaacagact
atgagcttaa agagatagtg gggatgatgg acgcgagtga 1380gaagaccaac
tacacgtgtt gcaggttgca aagacatgag tggaataaag gttggtgtaa
1440ctggtttcat atagaaccgt ggatatggtt gatgaacaaa acccaaaaca
acctgacaga 1500agggcaaccg cttagggagt gtgctgtgac ttgtaggtat
gacaaggaaa cagaattgaa 1560catcgtgaca caggctaggg acagacctac
aactctgaca ggttgcaaga aaggcaagaa 1620tttctctttc gcaggtgtta
tactggatgg gccctgtaac tttaaagtat cggttgaaga 1680tgtgctgttc
aaggagcacg attgcggcaa catgctgcaa gagaccgcga tacagctact
1740cgatggggca accaacacca ttgagggagc aagggtaggg acggccaagt
tgacaacctg 1800gttagggaag caattaggga tccttggtaa gaagttggag
aacaaaagca aagcatggtt 1860tggtgcacat gcagcaagtc catactgcgg
agtggagagg aagatcggtt acgtatggta 1920tacaaaaaac tgcactccag
cttgccttcc aagaaacact agaataatag gccccgggaa 1980atttgatacc
aacgccgaag atggaaaaat actccatgag atgggggggc acctctcaga
2040atttgtccta ttgtccttgg tggttctgtc tgactttgcc ccggaaaccg
cgagcgtcat 2100ctacttggtt ctacattttg cgatcccgca aagccacgtt
gatgtagaca catgcgacaa 2160gaaccagctg aatttaacgg tagcaaccac
agtagcagag gtcataccag ggacagtgtg 2220gaacctaggg aagtatgtct
gcataagacc agactggtgg ccatatgaga cgacgacagt 2280cttcgtcata
gaggaagcag ggcaagtaat caaattgatg ctaagggcca tcagagactt
2340aactaggata tggaatgctg ccactaccac agctttctta atctttttag
taaaagcact 2400gaggggacaa ctaatccaag ggctattgtg gctgatgcta
ataacaggag cacagggctt 2460ccctgaatgc aaagagggct tccaatatgc
catatctaaa gacaggaaaa tggggttatt 2520ggggccagag agcttaacta
caacatggca cctccccacc aaaaaaatag tggattccat 2580ggtgcatgta
tggtgtgaag gaaaagactt gaaaatatta aaaatgtgca caaaggaaga
2640gaggtatcta gtggctgtgc acgagagagc cttatcaacc agtgccgagt
ttatgcagat 2700cagtgatggg acaataggcc cagacgtgat agatatgcct
gatgactttg agtttggact 2760ctgcccttgt gactcaaaac cagtgataaa
gggcaaattt aatgccagct tactgaatgg 2820accagctttc cagatggtat
gcccacaggg gtggactggt acaatagaat gcaccctagc 2880gaaccaagac
accttggaca caactgtcat taggacatat agaagaacta ccccatttca
2940gcggagaaaa tggtgtacct atgaaaaaat aataggggaa gatatctatg
aatgcattct 3000aggtggaaac tggacatgca taaccggtga ccatagcagg
ttgaaagacg gacctatcaa 3060gaagtgtaag tggtgtggcc atgacttcgt
caactcagag gggctaccac actacccaat 3120aggcaagtgc atgctcatca
acgagagtgg gtacaggtat gtagatgaca cctcttgcga 3180taggggtggt
gtagccatag ttccatctgg caccgtaaag tgtagaatag gtaacgtcac
3240ggtgcaagtt atcgctacta acaatgatct gggacccatg ccttgcagcc
cagctgaagt 3300gatagcaagt gaaggaccag tggaaaagac tgcatgcaca
ttcaactatt caaggactct 3360acctaataag tattatgagc caagggaccg
gtacttccaa caatacatgt taaaagggga 3420gtggcaatat tggttcgacc
tggattctgt agaccaccac aaagactact tctcagagtt 3480cataatcata
gcagtggtcg ccttgttggg tggtaagtac gtactgtggc tcttgataac
3540atacacaata ctgtctgagc agatggctat gggtgctgga gtgaatactg
aagagatagt 3600catgataggc aatttgctga cagacagtga tattgaggtt
gtggtttatt tccttcttct 3660gtacttaata gttaaagagg aactggcgag
gaaatggatt atactggtat accacatcct 3720tgtagccaac cctatgaaaa
caattggggt cgtcttacta atgctagggg gagtggtgaa 3780ggccagcaga
atcaatgctg atgaccaaag tgctatggac ccatgctttc ttctcgtgac
3840aggcgtagtg gctgttttga tgatcgctag aagagaacct gccacattac
cactgattgt 3900agcattgcta gcaataagaa catcaggatt cctactgccc
gctagcattg atgtaactgt 3960agcagtagta ttaattgtac ttttgttggc
tagctacata acagactact ttagatataa 4020aaagtggctt caactcttat
ttagtctgat agctggtatc tttattataa ggagcttaaa 4080acatatcaac
cagatggagg taccagaaat atctatgcca agttggagac ctctagctct
4140ggtccttttc tatataacat ctacagcaat aaccactaat tgggacattg
acttagcagg 4200cttcctgctg caatgggcgc cagcagtgat catgatggct
accatgtggg cagacttttt 4260gactctgatc atagtcctgc ccagttacga
gttatctaag ctttacttcc taaagaacgt 4320caggacagac gtggaaaaga
actggctcgg caaagtgaaa tacagacaga tcagttcagt 4380ttatgacatc
tgtgacagtg aggaagcagt gtacctattt ccatcaaggc ataagagtgg
4440aagcaggcca gatttcatat tacctttttt gaaagccgtg ttaataagct
gcatcagcag 4500ccaatggcaa gtggtttaca tttcttacct aatactggaa
attacatact atatgcacag 4560gaaaatcata gatgaggtgt caggaggagc
aaattttcta tcaagactca tagcagccat 4620catagaatta aattgggcca
tagatgatga ggaatgtaaa ggactgaaga aactgtatct 4680cttgtcaggg
agagcgaaga atttgatagt taaacataag gtaagaaatg aagccgtcca
4740cagatggttt ggtgaggagg aaatatacgg ggcacccaag gtgatcacta
tcataaaagc 4800tagtacccta agtaaaaaca ggcactgcat aatctgcacg
atctgtgaag ggaaagaatg 4860gaatggagcc aactgcccaa agtgtggaag
acaaggaaag cccataacat gtggaatgac 4920actcgcagac tttgaggaga
aacattacaa aaagatattt ataagagaag aatcttcttg 4980tcctgtgcct
tttgatcctt cttgccattg taattatttt cgccacgatg ggcctttcag
5040gaaagagtat aagggttacg tccaatacac agccagagga caactctttc
tgaggaacct 5100accaattcta gcgacgaaga tgaagctatt aatggtggga
aacctcggcg cagaaattgg 5160cgacctggaa catctaggat gggtactgag
agggccagcc gtgtgcaaaa aaattaccaa 5220ccatgagaag tgccacgtaa
acatcatgga taagctaact gcattttttg gaatcatgcc 5280tagaggcacg
acccctaggg cacctgtgag gttccccaca gcactactaa aagtgagaag
5340ggggctagag acgggatggg cttacacgca ccaaggaggg atcagctcgg
tagaccatgt 5400cacagccgga aaggatttac tagtgtgtga cagtatgggc
aggaccaggg ttgtctgtca 5460tagtaacaat aagatgactg atgagactga
gtatggcatc aagaccgact cagggtgtcc 5520cgaaggtgcg aggtgttacg
tgctaaaccc agaagctgtt aacatttctg gcacaaaagg 5580agctatggta
cacctccaga aaacgggggg ggagttcaca tgtgtcactg cctcagggac
5640cccggctttc ttcgatctga aaaatctaaa aggctggtcc gggctaccaa
tttttgaagc 5700atccagtggc agggtggttg gtagggtgaa agtcggcaag
aatgaggatt
ccaagcccac 5760caaactaatg agcggaatcc agacagtgtc taagaaccag
acagacctag cggacatcgt 5820aaaaaaattg actagtatga acagaggaga
gttcaaacag ataacattag ccactggggc 5880aggaaaaact acggaactgc
caaggtccgt catagaggag atagggaggc acaaaagggt 5940cttagtcctg
ataccattga gagcagcagc agagtcagtg tatcagtata tgagagtgaa
6000gtacccaagt atatctttca atttgagaat aggagatatg aaggaaggtg
acatggccac 6060tggtatcacc tacgcctcat atgggtactt ttgtcagctt
cctcagccca aactgagagc 6120tgccatggta gagtactcat atatattctt
agatgagtac cactgtgcta cacccgagca 6180attagcaata attggaaaga
tacacaggtt tgctgaaaat cttagagtgg tagcaatgac 6240agcaacccca
gctggaacgg tcacaacgac tggtcagaaa caccctatag aggagttcat
6300agccccagag gtgatgaaag gtgaagatct aggtagtgaa tacttggata
ttgcagggtt 6360gaagataccg actgaagaga tgaaaggcaa catgctcgtg
ttcgcgccaa ctaggaacat 6420ggcagtagaa acagctaaga aattgaaggc
taagggatac aactctggat actattacag 6480tggggaaaac ccagagaact
tgagggtggt aacctcgcaa tccccgtatg tggtagtagc 6540caccaatgcc
atagagtcag gtgtgacatt accagactta gacacagttg tagacactgg
6600actaaagtgt gagaagaggg tgaggatttc ttcaaaaatg cccttcattg
taacaggact 6660taagagaatg gcagtcacaa tcggagagca agcccagcgc
aggggtagag taggaagagt 6720caagccaggt aggtactata ggagtcaaga
aacagcttca gggtcaaaag attaccatta 6780cgacctactg caagcccaga
ggtacggaat agaagatgga attaatgtaa caaagtcatt 6840cagggagatg
aactatgatt ggagccttta cgaagaggac agcttgatga taactcaact
6900cgaggtcctt aacaacctcc ttatatcaga agacctgcct gccgcagtga
agaacatcat 6960ggcccggacc gatcacccag aacccataca actggcctat
aacagttatg aaaaccaaat 7020tccagtgctg ttcccaaaga tcaaaaatgg
tgaggtgaca gacagttatg agaattacac 7080atatctcaat gcaagaaaat
taggagagga cgtgccggca tatgtgtacg ccacagagga 7140tgaggatcta
gcagtggatc ttctgggtat ggattggccg gacccaggca accaacaggt
7200ggtagagaca gggagggcat taaaacaagt aactggctta tccacagcag
aaaacgccct 7260cttgatagcc ctattcggct acgtcgggta ccagacactt
tcaaaaaggc acatacccat 7320gattactgac atctatacac ttgaagacca
caggcttgag gacacaaccc acctccagtt 7380tgccccaaac gctataagga
ccgacggcaa ggactcagag ttgaaggaat tagctgtggg 7440agaccttgat
aaatatgtgg acgcactggt agactactcc aaacaaggga tgaaattcat
7500caaagtccaa gctgaaaagg tcagagactc ccagtctacg aaggaaggct
tgcaaaccat 7560taaggagtat gtggataagt ttatacaatc actaacagag
aataaggagg agatcatcag 7620gtatggacta tggggagttc acacggcact
ctacaaaagc ttggcagcga gactggggca 7680tgaaacagct tttgcaactt
tagtggtaaa atggttggct tttgggggcg aaacggtatc 7740tgctcacatc
aagcaagtag cagttgatct agtagtatat tatatcatca acaaaccatc
7800ttttcctgga gatacagaga cccaacaaga ggggaggaag tttgtggcta
gtctttttat 7860atctgcacta gcaacataca catataaaac ctggaattac
aacaatctgc aacgggttgt 7920cgaacctgcc ttagcttacc tcccatatgc
tacaagtgcc ttgaagttgt tcacacccac 7980aagattagag agtgtggtca
tactcagttc tacaatttac aagacatacc tctctataag 8040gaagggtaag
agtgacggct tgttaggtac aggcataagt gcagccatgg agatcttaaa
8100ccaaaaccca atctcagtag gtatatctgt gatgctgggg gtaggtgcca
tcgccgccca 8160taatgcaata gaatctagtg aacagaaaag aactttgctg
atgaaggtct ttgtaaaaaa 8220cttcttagac caagcagcaa cagatgagct
agtcaaagag aaccctgaaa aaataatcat 8280ggctctattt gaagcagtcc
agaccatagg aaacccccta agactcatct accatctgta 8340cggggtgtac
tataaggggt gggaagcaaa agaactcgca gagaaaactg ctggccgcaa
8400cttattcaca ttgatcatgt ttgaggcctt tgagctttta ggtatggact
cagaaggaaa 8460gataagaaac ttgtcaggca actacatact ggacttaatc
ttcaacttgc ataataaatt 8520aaacaagggg ctcaaaaaac tagtccttgg
gtgggctcct gcacctttga gctgtgattg 8580gacaccaagt gatgagagaa
taagcctacc tcataacaac tacttaaggg tagaaaccag 8640gtgtccttgt
ggctatgaga tgaaggcaat aaaaaatgtt gctggtaaat tgacaaaagt
8700tgaagaaaag gggtccttcc tatgcaggaa tagattaggg agaggacctc
caaacttcaa 8760agtaacaaag ttctatgatg ataacttgat agaagtcaag
ccagtagcta ggctagaagg 8820ccaggtggac ctctattaca agggagtaac
agctaagtta gactacaaca atgggaaagt 8880actgttagct accaacaagt
gggaggtgga ccacgctttc ctgaccagac tagtaaagaa 8940gcacacaggg
ataggtttta aaggtgcata tttgggtgac cgaccagacc atcaagatct
9000tgtcgataga gattgtgcaa ctataacgaa gaactcagta cagttcctaa
aaatgaagaa 9060gggttgcgct ttcacatatg acctaacaat ctctaacctt
gtcaggctta ttgaactagt 9120ccataagaat aatttacaag aaagagagat
ccctaccgtg acagtaacta cttggcttgc 9180atattctttt gtcaatgaag
acctggggac tatcaagcct gtattggggg agaaagtcat 9240cccagaaccc
cccgaggagt tgagtctcca acccaccgtg agactagtca ccactgaaac
9300agcaataacc ataacagggg aggctgaagt gatgacgaca gggatcacac
cagtggtaga 9360gatgaaagaa gaacctcagc tggaccacca gtcaactacc
ctaaaggtag ggttgaagga 9420aggggaatat ccagggccag gagttaaccc
taaccattta gcagaggtga tagatgagaa 9480agatgacagg ccttttgtcc
taatcatcgg taacaaaggt tctacctcga acagagcaag 9540aacggccaag
aatatacggc tgtacaaagg aaacaaccca agagagatca gggatctgat
9600gagccaagga agaatattga cggttgctct aaaagagttg gacccggaat
taaaagaatt 9660agtagattac aaggggacct ttctcaatag ggaagcttta
gaagccctaa gcttaggtaa 9720gccaatcaag aggaaaacca caacagcaat
gatcaggagg ttaatagagc cagaggttga 9780ggaggaacta ccagattggt
tccaagcgga agaaccccta tttttggaag caaaaataca 9840gaatgactta
taccacctaa ttggcagtgt agatagtata aaaagcaaag caaaggaatt
9900aggggccaca gataacacaa agatagtgaa ggaagttggg gctaggacct
atacgatgaa 9960attgagcagc tggagcacac aagttacaaa aaaacagatg
agtctagccc ctctctttga 10020agagctgtta ttaaagtgcc ctccatgtag
taaaatttca aagggacata tggtgtcagc 10080ataccaactg gctcaaggaa
actgggaacc cctcgggtgt ggggtctata tgggaaccat 10140accagctagg
cgtctcaaga tccaccctta tgaggcttac cttaaactca aagagctggt
10200ggaagttgaa tcttcgaggg ccactgcaaa agaatccatc ataagagaac
ataacacctg 10260gatcctgcgg aaggtgagac atgaagggaa cctaagaacc
aaatcaatga tcaaccctgg 10320gaaaatatca gatcagctat gcagagatgg
acacaaaaga aacatatata ataagatcat 10380aggctcaaca atggcctctg
ctggtattag gctggagaaa ctgccagtag tccgagccca 10440aactgacaca
accagtttcc accaagccat aagagaaaaa attgataaaa cagaaaacaa
10500gcagacccct gaattgcatg aagaactaat gaaggtcttc gactgcttaa
agatcccaga 10560gctgaaggaa tcgtatgatg aagtttcatg ggaacaatta
gaagccggga taaaccgtaa 10620gggtgcagca ggctatctag agagcaagaa
cataggggaa gtcctagaca cagagaaaca 10680catagtagag cagctgatca
aggatctgag gaaggggaag aagattaggt actatgaaac 10740agccatcccc
aagaatgaga agagagacgt cagcgacgac tgggaagccg gagagttcgt
10800tgatgaaaag aaaccaagag taatccagta cccggacgcc aaggtgagac
tggccattac 10860aaaagtgatg tacaaatggg taaagcaaaa accagtggtg
atacccggct atgaaggtaa 10920aacacctcta tttgacatat tcaacaaagt
gaagaaggaa tgggattcat tccaggaccc 10980cgtagcagtg agctttgaca
ccaaagcgtg ggatacacaa gtcaccagta gagacctaat 11040gttgataaag
gatatccaga aatattattt caagagaagt atacacaaat ttttagatac
11100aataacagaa cacatggtgg aggtacctgt cattacagca gacggtgaag
tttacataag 11160gaatggtcag aggggtagtg gccaacccga cacaagtgct
ggtaatagta tgttgaatgt 11220cctaaccatg atatatgctt tctgtaaaag
tacaggcata ccttacaggg gattcagcag 11280agtggcaaga atccatgtgt
gtggtgatga tggctttttg ataacagaga gaggactggg 11340actgaaattc
tctgagaagg gtatgcagat attacatgag gccgggaagc cccagaaaat
11400aactgaaggg gacaaaatga aagtggcata cagattcgag gacatagagt
tttgttccca 11460tactcccgtg ccagtcagat gggcagataa caccagtagt
tacatggcag ggaggagcac 11520agccactata ctagctaaga tggcaaccag
gctggattcc agcggagaga ggggtagcac 11580agcttatgag aaggccgtag
ccttcagctt ccttttgatg tactcatgga atcccgtagt 11640tagaaggatc
tgcttactgg tgttgtcaca gtttccagaa atatccccat ccaaaaacac
11700aatatactac taccaagggg atcccatagc tgcgtacaga gaagtgatag
ggaaacagct 11760gtgtgaactg aaaagaacag gatttgagaa gctggctggt
ctgaatttga gtatgaccac 11820tctaggcatc tggacaaaac atactagtaa
aagactaatc caagcctgtg tagaaatagg 11880taagagagaa ggtacctggt
tagttaatgc tgacagactg attgcaggaa agactgggaa 11940gttttacatc
ccaagcactg gtgtcactct gttgggaaaa cactatgagg aaattaactt
12000aaagcaaaag gcggcacaac cgccgataga gggggttgac agatataagt
tgggccccat 12060agttaatgtt atcttgagaa ggctgagggt gatgctgatg
acagttgcca gcggaagctg 12120gtgaatccgt ccggagcgtc gtgccctcac
tcaaggtttt taattgtaaa tattgtaaat 12180agacagctaa gatatttatt
gtagttggat agtaatgcag tgatagtaaa taccccaatt 12240taacactacc
tccaatgcac taagcacttt agctgtgtga ggttaactcg acgtccacgg
12300ttggactagg gaagacctct aacagcccc 12329411837DNAArtificial
SequenceMutated BVDV XIKE-B-NdN 4gtatacgaga ttagctaaag aactcgtata
tggattggac gtcaacaaat ttttaattgg 60caacgtaggg aaccttcccc tcagcgaagg
ccgaaaagag gctagccatg cccttagtag 120gactagcaaa agtaggggac
tagcggtagc agtgagttcg ttggatggcc gaacccctga 180gtacagggga
gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg
240tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg
ggtgaaagcg 300ctattcgtgg cgttatggac acagcctgat agggtgtagc
agagacctgc tattccgcta 360gtaaaaactc tgctgtacat ggcacatgga
gttgttttcc gatgaaggga gcaagggtgc 420tacaagtaag aagcagccta
agccagatag gatagaaaaa ggtaagatga aaatagcccc 480aaaagagaca
gaaaaagatt gcaaaaccag accccccgac gcgactatag tagtagaagg
540ggttaagtac caggtgaaga aaaaaggaaa ggtaagggga aaaaatactc
aagatgggtt 600atatcacaac aagaataagc cccctgaatc aagaaaaaaa
ttggaaaagg cactgctggc 660ttgggccatc ttagcagcgg tcctgcttca
gctggtaaca ggagagaata tcacccagtg 720gaacttgatg gacaacggca
ccgagggaat acagcaagcg atgttcctaa gaggggtgaa 780caggagtcta
catggaattt ggccagagaa aatttgcacc ggagtaccaa ctcacttagc
840aacagactat gagcttaaag agatagtggg gatgatggac gcgagtgaga
agaccaacta 900cacgtgttgc aggttgcaaa gacatgagtg gaataaaggt
tggtgtaact ggtttcatat 960agaaccgtgg atatggttga tgaacaaaac
ccaaaacaac ctgacagaag ggcaaccgct 1020tagggagtgt gctgtgactt
gtaggtatga caaggaaaca gaattgaaca tcgtgacaca 1080ggctagggac
agacctacaa ctctgacagg ttgcaagaaa ggcaagaatt tctctttcgc
1140aggtgttata ctggatgggc cctgtaactt taaagtatcg gttgaagatg
tgctgttcaa 1200ggagcacgat tgcggcaaca tgctgcaaga gaccgcgata
cagctactcg atggggcaac 1260caacaccatt gagggagcaa gggtagggac
ggccaagttg acaacctggt tagggaagca 1320attagggatc cttggtaaga
agttggagaa caaaagcaaa gcatggtttg gtgcacatgc 1380agcaagtcca
tactgcggag tggagaggaa gatcggttac gtatggtata caaaaaactg
1440cactccagct tgccttccaa gaaacactag aataataggc cccgggaaat
ttgataccaa 1500cgccgaagat ggaaaaatac tccatgagat gggggggcac
ctctcagaat ttgtcctatt 1560gtccttggtg gttctgtctg actttgcccc
ggaaaccgcg agcgtcatct acttggttct 1620acattttgcg atcccgcaaa
gccacgttga tgtagacaca tgcgacaaga accagctgaa 1680tttaacggta
gcaaccacag tagcagaggt cataccaggg acagtgtgga acctagggaa
1740gtatgtctgc ataagaccag actggtggcc atatgagacg acgacagtct
tcgtcataga 1800ggaagcaggg caagtaatca aattgatgct aagggccatc
agagacttaa ctaggatatg 1860gaatgctgcc actaccacag ctttcttaat
ctttttagta aaagcactga ggggacaact 1920aatccaaggg ctattgtggc
tgatgctaat aacaggagca cagggcttcc ctgaatgcaa 1980agagggcttc
caatatgcca tatctaaaga caggaaaatg gggttattgg ggccagagag
2040cttaactaca acatggcacc tccccaccaa aaaaatagtg gattccatgg
tgcatgtatg 2100gtgtgaagga aaagacttga aaatattaaa aatgtgcaca
aaggaagaga ggtatctagt 2160ggctgtgcac gagagagcct tatcaaccag
tgccgagttt atgcagatca gtgatgggac 2220aataggccca gacgtgatag
atatgcctga tgactttgag tttggactct gcccttgtga 2280ctcaaaacca
gtgataaagg gcaaatttaa tgccagctta ctgaatggac cagctttcca
2340gatggtatgc ccacaggggt ggactggtac aatagaatgc accctagcga
accaagacac 2400cttggacaca actgtcatta ggacatatag aagaactacc
ccatttcagc ggagaaaatg 2460gtgtacctat gaaaaaataa taggggaaga
tatctatgaa tgcattctag gtggaaactg 2520gacatgcata accggtgacc
atagcaggtt gaaagacgga cctatcaaga agtgtaagtg 2580gtgtggccat
gacttcgtca actcagaggg gctaccacac tacccaatag gcaagtgcat
2640gctcatcaac gagagtgggt acaggtatgt agatgacacc tcttgcgata
ggggtggtgt 2700agccatagtt ccatctggca ccgtaaagtg tagaataggt
aacgtcacgg tgcaagttat 2760cgctactaac aatgatctgg gacccatgcc
ttgcagccca gctgaagtga tagcaagtga 2820aggaccagtg gaaaagactg
catgcacatt caactattca aggactctac ctaataagta 2880ttatgagcca
agggaccggt acttccaaca atacatgtta aaaggggagt ggcaatattg
2940gttcgacctg gattctgtag accaccacaa agactacttc tcagagttca
taatcatagc 3000agtggtcgcc ttgttgggtg gtaagtacgt actgtggctc
ttgataacat acacaatact 3060gtctgagcag atggctatgg gtgctggagt
gaatactgaa gagatagtca tgataggcaa 3120tttgctgaca gacagtgata
ttgaggttgt ggtttatttc cttcttctgt acttaatagt 3180taaagaggaa
ctggcgagga aatggattat actggtatac cacatccttg tagccaaccc
3240tatgaaaaca attggggtcg tcttactaat gctaggggga gtggtgaagg
ccagcagaat 3300caatgctgat gaccaaagtg ctatggaccc atgctttctt
ctcgtgacag gcgtagtggc 3360tgttttgatg atcgctagaa gagaacctgc
cacattacca ctgattgtag cattgctagc 3420aataagaaca tcaggattcc
tactgcccgc tagcattgat gtaactgtag cagtagtatt 3480aattgtactt
ttgttggcta gctacataac agactacttt agatataaaa agtggcttca
3540actcttattt agtctgatag ctggtatctt tattataagg agcttaaaac
atatcaacca 3600gatggaggta ccagaaatat ctatgccaag ttggagacct
ctagctctgg tccttttcta 3660tataacatct acagcaataa ccactaattg
ggacattgac ttagcaggct tcctgctgca 3720atgggcgcca gcagtgatca
tgatggctac catgtgggca gactttttga ctctgatcat 3780agtcctgccc
agttacgagt tatctaagct ttacttccta aagaacgtca ggacagacgt
3840ggaaaagaac tggctcggca aagtgaaata cagacagatc agttcagttt
atgacatctg 3900tgacagtgag gaagcagtgt acctatttcc atcaaggcat
aagagtggaa gcaggccaga 3960tttcatatta ccttttttga aagccgtgtt
aataagctgc atcagcagcc aatggcaagt 4020ggtttacatt tcttacctaa
tactggaaat tacatactat atgcacagga aaatcataga 4080tgaggtgtca
ggaggagcaa attttctatc aagactcata gcagccatca tagaattaaa
4140ttgggccata gatgatgagg aatgtaaagg actgaagaaa ctgtatctct
tgtcagggag 4200agcgaagaat ttgatagtta aacataaggt aagaaatgaa
gccgtccaca gatggtttgg 4260tgaggaggaa atatacgggg cacccaaggt
gatcactatc ataaaagcta gtaccctaag 4320taaaaacagg cactgcataa
tctgcacgat ctgtgaaggg aaagaatgga atggagccaa 4380ctgcccaaag
tgtggaagac aaggaaagcc cataacatgt ggaatgacac tcgcagactt
4440tgaggagaaa cattacaaaa agatatttat aagagaagaa tcttcttgtc
ctgtgccttt 4500tgatccttct tgccattgta attattttcg ccacgatggg
cctttcagga aagagtataa 4560gggttacgtc caatacacag ccagaggaca
actctttctg aggaacctac caattctagc 4620gacgaagatg aagctattaa
tggtgggaaa cctcggcgca gaaattggcg acctggaaca 4680tctaggatgg
gtactgagag ggccagccgt gtgcaaaaaa attaccaacc atgagaagtg
4740ccacgtaaac atcatggata agctaactgc attttttgga atcatgccta
gaggcacgac 4800ccctagggca cctgtgaggt tccccacagc actactaaaa
gtgagaaggg ggctagagac 4860gggatgggct tacacgcacc aaggagggat
cagctcggta gaccatgtca cagccggaaa 4920ggatttacta gtgtgtgaca
gtatgggcag gaccagggtt gtctgtcata gtaacaataa 4980gatgactgat
gagactgagt atggcatcaa gaccgactca gggtgtcccg aaggtgcgag
5040gtgttacgtg ctaaacccag aagctgttaa catttctggc acaaaaggag
ctatggtaca 5100cctccagaaa acgggggggg agttcacatg tgtcactgcc
tcagggaccc cggctttctt 5160cgatctgaaa aatctaaaag gctggtccgg
gctaccaatt tttgaagcat ccagtggcag 5220ggtggttggt agggtgaaag
tcggcaagaa tgaggattcc aagcccacca aactaatgag 5280cggaatccag
acagtgtcta agaaccagac agacctagcg gacatcgtaa aaaaattgac
5340tagtatgaac agaggagagt tcaaacagat aacattagcc actggggcag
gaaaaactac 5400ggaactgcca aggtccgtca tagaggagat agggaggcac
aaaagggtct tagtcctgat 5460accattgaga gcagcagcag agtcagtgta
tcagtatatg agagtgaagt acccaagtat 5520atctttcaat ttgagaatag
gagatatgaa ggaaggtgac atggccactg gtatcaccta 5580cgcctcatat
gggtactttt gtcagcttcc tcagcccaaa ctgagagctg ccatggtaga
5640gtactcatat atattcttag atgagtacca ctgtgctaca cccgagcaat
tagcaataat 5700tggaaagata cacaggtttg ctgaaaatct tagagtggta
gcaatgacag caaccccagc 5760tggaacggtc acaacgactg gtcagaaaca
ccctatagag gagttcatag ccccagaggt 5820gatgaaaggt gaagatctag
gtagtgaata cttggatatt gcagggttga agataccgac 5880tgaagagatg
aaaggcaaca tgctcgtgtt cgcgccaact aggaacatgg cagtagaaac
5940agctaagaaa ttgaaggcta agggatacaa ctctggatac tattacagtg
gggaaaaccc 6000agagaacttg agggtggtaa cctcgcaatc cccgtatgtg
gtagtagcca ccaatgccat 6060agagtcaggt gtgacattac cagacttaga
cacagttgta gacactggac taaagtgtga 6120gaagagggtg aggatttctt
caaaaatgcc cttcattgta acaggactta agagaatggc 6180agtcacaatc
ggagagcaag cccagcgcag gggtagagta ggaagagtca agccaggtag
6240gtactatagg agtcaagaaa cagcttcagg gtcaaaagat taccattacg
acctactgca 6300agcccagagg tacggaatag aagatggaat taatgtaaca
aagtcattca gggagatgaa 6360ctatgattgg agcctttacg aagaggacag
cttgatgata actcaactcg aggtccttaa 6420caacctcctt atatcagaag
acctgcctgc cgcagtgaag aacatcatgg cccggaccga 6480tcacccagaa
cccatacaac tggcctataa cagttatgaa aaccaaattc cagtgctgtt
6540cccaaagatc aaaaatggtg aggtgacaga cagttatgag aattacacat
atctcaatgc 6600aagaaaatta ggagaggacg tgccggcata tgtgtacgcc
acagaggatg aggatctagc 6660agtggatctt ctgggtatgg attggccgga
cccaggcaac caacaggtgg tagagacagg 6720gagggcatta aaacaagtaa
ctggcttatc cacagcagaa aacgccctct tgatagccct 6780attcggctac
gtcgggtacc agacactttc aaaaaggcac atacccatga ttactgacat
6840ctatacactt gaagaccaca ggcttgagga cacaacccac ctccagtttg
ccccaaacgc 6900tataaggacc gacggcaagg actcagagtt gaaggaatta
gctgtgggag accttgataa 6960atatgtggac gcactggtag actactccaa
acaagggatg aaattcatca aagtccaagc 7020tgaaaaggtc agagactccc
agtctacgaa ggaaggcttg caaaccatta aggagtatgt 7080ggataagttt
atacaatcac taacagagaa taaggaggag atcatcaggt atggactatg
7140gggagttcac acggcactct acaaaagctt ggcagcgaga ctggggcatg
aaacagcttt 7200tgcaacttta gtggtaaaat ggttggcttt tgggggcgaa
acggtatctg ctcacatcaa 7260gcaagtagca gttgatctag tagtatatta
tatcatcaac aaaccatctt ttcctggaga 7320tacagagacc caacaagagg
ggaggaagtt tgtggctagt ctttttatat ctgcactagc 7380aacatacaca
tataaaacct ggaattacaa caatctgcaa cgggttgtcg aacctgcctt
7440agcttacctc ccatatgcta caagtgcctt gaagttgttc acacccacaa
gattagagag 7500tgtggtcata ctcagttcta caatttacaa gacatacctc
tctataagga agggtaagag 7560tgacggcttg ttaggtacag gcataagtgc
agccatggag atcttaaacc aaaacccaat 7620ctcagtaggt atatctgtga
tgctgggggt aggtgccatc gccgcccata atgcaataga 7680atctagtgaa
cagaaaagaa ctttgctgat gaaggtcttt gtaaaaaact tcttagacca
7740agcagcaaca gatgagctag tcaaagagaa ccctgaaaaa ataatcatgg
ctctatttga 7800agcagtccag accataggaa accccctaag actcatctac
catctgtacg gggtgtacta 7860taaggggtgg gaagcaaaag aactcgcaga
gaaaactgct ggccgcaact tattcacatt 7920gatcatgttt gaggcctttg
agcttttagg tatggactca gaaggaaaga taagaaactt 7980gtcaggcaac
tacatactgg acttaatctt caacttgcat aataaattaa acaaggggct
8040caaaaaacta gtccttgggt gggctcctgc acctttgagc tgtgattgga
caccaagtga 8100tgagagaata agcctacctc ataacaacta cttaagggta
gaaaccaggt gtccttgtgg 8160ctatgagatg aaggcaataa aaaatgttgc
tggtaaattg acaaaagttg aagaaaaggg 8220gtccttccta tgcaggaata
gattagggag aggacctcca aacttcaaag taacaaagtt 8280ctatgatgat
aacttgatag aagtcaagcc agtagctagg ctagaaggcc aggtggacct
8340ctattacaag ggagtaacag ctaagttaga ctacaacaat gggaaagtac
tgttagctac
8400caacaagtgg gaggtggacc acgctttcct gaccagacta gtaaagaagc
acacagggat 8460aggttttaaa ggtgcatatt tgggtgaccg accagaccat
caagatcttg tcgatagaga 8520ttgtgcaact ataacgaaga actcagtaca
gttcctaaaa atgaagaagg gttgcgcttt 8580cacatatgac ctaacaatct
ctaaccttgt caggcttatt gaactagtcc ataagaataa 8640tttacaagaa
agagagatcc ctaccgtgac agtaactact tggcttgcat attcttttgt
8700caatgaagac ctggggacta tcaagcctgt attgggggag aaagtcatcc
cagaaccccc 8760cgaggagttg agtctccaac ccaccgtgag actagtcacc
actgaaacag caataaccat 8820aacaggggag gctgaagtga tgacgacagg
gatcacacca gtggtagaga tgaaagaaga 8880acctcagctg gaccaccagt
caactaccct aaaggtaggg ttgaaggaag gggaatatcc 8940agggccagga
gttaacccta accatttagc agaggtgata gatgagaaag atgacaggcc
9000ttttgtccta atcatcggta acaaaggttc tacctcgaac agagcaagaa
cggccaagaa 9060tatacggctg tacaaaggaa acaacccaag agagatcagg
gatctgatga gccaaggaag 9120aatattgacg gttgctctaa aagagttgga
cccggaatta aaagaattag tagattacaa 9180ggggaccttt ctcaataggg
aagctttaga agccctaagc ttaggtaagc caatcaagag 9240gaaaaccaca
acagcaatga tcaggaggtt aatagagcca gaggttgagg aggaactacc
9300agattggttc caagcggaag aacccctatt tttggaagca aaaatacaga
atgacttata 9360ccacctaatt ggcagtgtag atagtataaa aagcaaagca
aaggaattag gggccacaga 9420taacacaaag atagtgaagg aagttggggc
taggacctat acgatgaaat tgagcagctg 9480gagcacacaa gttacaaaaa
aacagatgag tctagcccct ctctttgaag agctgttatt 9540aaagtgccct
ccatgtagta aaatttcaaa gggacatatg gtgtcagcat accaactggc
9600tcaaggaaac tgggaacccc tcgggtgtgg ggtctatatg ggaaccatac
cagctaggcg 9660tctcaagatc cacccttatg aggcttacct taaactcaaa
gagctggtgg aagttgaatc 9720ttcgagggcc actgcaaaag aatccatcat
aagagaacat aacacctgga tcctgcggaa 9780ggtgagacat gaagggaacc
taagaaccaa atcaatgatc aaccctggga aaatatcaga 9840tcagctatgc
agagatggac acaaaagaaa catatataat aagatcatag gctcaacaat
9900ggcctctgct ggtattaggc tggagaaact gccagtagtc cgagcccaaa
ctgacacaac 9960cagtttccac caagccataa gagaaaaaat tgataaaaca
gaaaacaagc agacccctga 10020attgcatgaa gaactaatga aggtcttcga
ctgcttaaag atcccagagc tgaaggaatc 10080gtatgatgaa gtttcatggg
aacaattaga agccgggata aaccgtaagg gtgcagcagg 10140ctatctagag
agcaagaaca taggggaagt cctagacaca gagaaacaca tagtagagca
10200gctgatcaag gatctgagga aggggaagaa gattaggtac tatgaaacag
ccatccccaa 10260gaatgagaag agagacgtca gcgacgactg ggaagccgga
gagttcgttg atgaaaagaa 10320accaagagta atccagtacc cggacgccaa
ggtgagactg gccattacaa aagtgatgta 10380caaatgggta aagcaaaaac
cagtggtgat acccggctat gaaggtaaaa cacctctatt 10440tgacatattc
aacaaagtga agaaggaatg ggattcattc caggaccccg tagcagtgag
10500ctttgacacc aaagcgtggg atacacaagt caccagtaga gacctaatgt
tgataaagga 10560tatccagaaa tattatttca agagaagtat acacaaattt
ttagatacaa taacagaaca 10620catggtggag gtacctgtca ttacagcaga
cggtgaagtt tacataagga atggtcagag 10680gggtagtggc caacccgaca
caagtgctgg taatagtatg ttgaatgtcc taaccatgat 10740atatgctttc
tgtaaaagta caggcatacc ttacagggga ttcagcagag tggcaagaat
10800ccatgtgtgt ggtgatgatg gctttttgat aacagagaga ggactgggac
tgaaattctc 10860tgagaagggt atgcagatat tacatgaggc cgggaagccc
cagaaaataa ctgaagggga 10920caaaatgaaa gtggcataca gattcgagga
catagagttt tgttcccata ctcccgtgcc 10980agtcagatgg gcagataaca
ccagtagtta catggcaggg aggagcacag ccactatact 11040agctaagatg
gcaaccaggc tggattccag cggagagagg ggtagcacag cttatgagaa
11100ggccgtagcc ttcagcttcc ttttgatgta ctcatggaat cccgtagtta
gaaggatctg 11160cttactggtg ttgtcacagt ttccagaaat atccccatcc
aaaaacacaa tatactacta 11220ccaaggggat cccatagctg cgtacagaga
agtgataggg aaacagctgt gtgaactgaa 11280aagaacagga tttgagaagc
tggctggtct gaatttgagt atgaccactc taggcatctg 11340gacaaaacat
actagtaaaa gactaatcca agcctgtgta gaaataggta agagagaagg
11400tacctggtta gttaatgctg acagactgat tgcaggaaag actgggaagt
tttacatccc 11460aagcactggt gtcactctgt tgggaaaaca ctatgaggaa
attaacttaa agcaaaaggc 11520ggcacaaccg ccgatagagg gggttgacag
atataagttg ggccccatag ttaatgttat 11580cttgagaagg ctgagggtga
tgctgatgac agttgccagc ggaagctggt gaatccgtcc 11640ggagcgtcgt
gccctcactc aaggttttta attgtaaata ttgtaaatag acagctaaga
11700tatttattgt agttggatag taatgcagtg atagtaaata ccccaattta
acactacctc 11760caatgcacta agcactttag ctgtgtgagg ttaactcgac
gtccacggtt ggactaggga 11820agacctctaa cagcccc 1183753913PRTWildtyp
BVDV XIKE-A 5Met Glu Leu Phe Ser Asn Glu Leu Leu Tyr Lys Thr Tyr
Lys Gln Lys 1 5 10 15 Pro Ala Gly Val Val Glu Pro Val Tyr Asp Val
Asn Gly Arg Pro Leu 20 25 30 Phe Gly Glu Ser Ser Asp Leu His Pro
Gln Ser Thr Leu Lys Leu Pro 35 40 45 His Gln Arg Gly Ser Ala Asn
Ile Leu Thr Asn Ala Arg Ser Leu Pro 50 55 60 Arg Lys Gly Asp Cys
Arg Arg Gly Asn Val Tyr Gly Pro Val Ser Gly 65 70 75 80 Ile Tyr Ile
Lys Pro Gly Pro Ile Tyr Tyr Gln Asp Tyr Val Gly Pro 85 90 95 Val
Tyr His Arg Ala Pro Leu Glu Leu Cys Arg Glu Ala Ser Met Cys 100 105
110 Glu Thr Thr Arg Arg Val Gly Arg Val Thr Gly Ser Asp Gly Lys Leu
115 120 125 Tyr His Ile Tyr Ile Cys Ile Asp Gly Cys Ile Leu Leu Lys
Arg Ala 130 135 140 Thr Arg Asn Gln Pro Glu Val Leu Lys Trp Val Tyr
Asn Arg Leu Asn 145 150 155 160 Cys Pro Leu Trp Val Thr Ser Cys Ser
Asp Glu Gly Ser Lys Gly Ala 165 170 175 Thr Ser Lys Lys Gln Pro Lys
Pro Asp Arg Ile Glu Lys Gly Lys Met 180 185 190 Lys Ile Ala Pro Lys
Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro 195 200 205 Asp Ala Thr
Ile Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys 210 215 220 Gly
Lys Val Arg Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys 225 230
235 240 Asn Lys Pro Pro Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu
Ala 245 250 255 Trp Ala Ile Leu Ala Ala Val Leu Leu Gln Leu Val Thr
Gly Glu Asn 260 265 270 Ile Thr Gln Trp Asn Leu Met Asp Asn Gly Thr
Glu Gly Ile Gln Gln 275 280 285 Ala Met Phe Leu Arg Gly Val Asn Arg
Ser Leu His Gly Ile Trp Pro 290 295 300 Glu Lys Ile Cys Thr Gly Val
Pro Thr His Leu Ala Thr Asp Tyr Glu 305 310 315 320 Leu Lys Glu Ile
Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr 325 330 335 Thr Cys
Cys Arg Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys 340 345 350
Asn Trp Phe His Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln 355
360 365 Asn Asn Leu Thr Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr
Cys 370 375 380 Arg Tyr Asp Lys Glu Thr Glu Leu Asn Ile Val Thr Gln
Ala Arg Asp 385 390 395 400 Arg Pro Thr Thr Leu Thr Gly Cys Lys Lys
Gly Lys Asn Phe Ser Phe 405 410 415 Ala Gly Val Ile Leu Asp Gly Pro
Cys Asn Phe Lys Val Ser Val Glu 420 425 430 Asp Val Leu Phe Lys Glu
His Asp Cys Gly Asn Met Leu Gln Glu Thr 435 440 445 Ala Ile Gln Leu
Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg 450 455 460 Val Gly
Thr Ala Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile 465 470 475
480 Leu Gly Lys Lys Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His
485 490 495 Ala Ala Ser Pro Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr
Val Trp 500 505 510 Tyr Thr Lys Asn Cys Thr Pro Ala Cys Leu Pro Arg
Asn Thr Arg Ile 515 520 525 Ile Gly Pro Gly Lys Phe Asp Thr Asn Ala
Glu Asp Gly Lys Ile Leu 530 535 540 His Glu Met Gly Gly His Leu Ser
Glu Phe Val Leu Leu Ser Leu Val 545 550 555 560 Val Leu Ser Asp Phe
Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val 565 570 575 Leu His Phe
Ala Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp 580 585 590 Lys
Asn Gln Leu Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile 595 600
605 Pro Gly Thr Val Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp
610 615 620 Trp Trp Pro Tyr Glu Thr Thr Thr Val Phe Val Ile Glu Glu
Ala Gly 625 630 635 640 Gln Val Ile Lys Leu Met Leu Arg Ala Ile Arg
Asp Leu Thr Arg Ile 645 650 655 Trp Asn Ala Ala Thr Thr Thr Ala Phe
Leu Ile Phe Leu Val Lys Ala 660 665 670 Leu Arg Gly Gln Leu Ile Gln
Gly Leu Leu Trp Leu Met Leu Ile Thr 675 680 685 Gly Ala Gln Gly Phe
Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile 690 695 700 Ser Lys Asp
Arg Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr 705 710 715 720
Thr Trp His Leu Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val 725
730 735 Trp Cys Glu Gly Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys
Glu 740 745 750 Glu Arg Tyr Leu Val Ala Val His Glu Arg Ala Leu Ser
Thr Ser Ala 755 760 765 Glu Phe Met Gln Ile Ser Asp Gly Thr Ile Gly
Pro Asp Val Ile Asp 770 775 780 Met Pro Asp Asp Phe Glu Phe Gly Leu
Cys Pro Cys Asp Ser Lys Pro 785 790 795 800 Val Ile Lys Gly Lys Phe
Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe 805 810 815 Gln Met Val Cys
Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu 820 825 830 Ala Asn
Gln Asp Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg 835 840 845
Thr Thr Pro Phe Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile 850
855 860 Gly Glu Asp Ile Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys
Ile 865 870 875 880 Thr Gly Asp His Ser Arg Leu Lys Asp Gly Pro Ile
Lys Lys Cys Lys 885 890 895 Trp Cys Gly His Asp Phe Val Asn Ser Glu
Gly Leu Pro His Tyr Pro 900 905 910 Ile Gly Lys Cys Met Leu Ile Asn
Glu Ser Gly Tyr Arg Tyr Val Asp 915 920 925 Asp Thr Ser Cys Asp Arg
Gly Gly Val Ala Ile Val Pro Ser Gly Thr 930 935 940 Val Lys Cys Arg
Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn 945 950 955 960 Asn
Asp Leu Gly Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser 965 970
975 Glu Gly Pro Val Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr
980 985 990 Leu Pro Asn Lys Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln
Gln Tyr 995 1000 1005 Met Leu Lys Gly Glu Trp Gln Tyr Trp Phe Asp
Leu Asp Ser Val 1010 1015 1020 Asp His His Lys Asp Tyr Phe Ser Glu
Phe Ile Ile Ile Ala Val 1025 1030 1035 Val Ala Leu Leu Gly Gly Lys
Tyr Val Leu Trp Leu Leu Ile Thr 1040 1045 1050 Tyr Thr Ile Leu Ser
Glu Gln Met Ala Met Gly Ala Gly Val Asn 1055 1060 1065 Thr Glu Glu
Ile Val Met Ile Gly Asn Leu Leu Thr Asp Ser Asp 1070 1075 1080 Ile
Glu Val Val Val Tyr Phe Leu Leu Leu Tyr Leu Ile Val Lys 1085 1090
1095 Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu Val Tyr His Ile Leu
1100 1105 1110 Val Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu Leu
Met Leu 1115 1120 1125 Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala
Asp Asp Gln Ser 1130 1135 1140 Ala Met Asp Pro Cys Phe Leu Leu Val
Thr Gly Val Val Ala Val 1145 1150 1155 Leu Met Ile Ala Arg Arg Glu
Pro Ala Thr Leu Pro Leu Ile Val 1160 1165 1170 Ala Leu Leu Ala Ile
Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser 1175 1180 1185 Ile Asp Val
Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala 1190 1195 1200 Ser
Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu 1205 1210
1215 Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys
1220 1225 1230 His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro
Ser Trp 1235 1240 1245 Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr
Ser Thr Ala Ile 1250 1255 1260 Thr Thr Asn Trp Asp Ile Asp Leu Ala
Gly Phe Leu Leu Gln Trp 1265 1270 1275 Ala Pro Ala Val Ile Met Met
Ala Thr Met Trp Ala Asp Phe Leu 1280 1285 1290 Thr Leu Ile Ile Val
Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr 1295 1300 1305 Phe Leu Lys
Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly 1310 1315 1320 Lys
Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp 1325 1330
1335 Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly
1340 1345 1350 Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val
Leu Ile 1355 1360 1365 Ser Cys Ile Ser Ser Gln Trp Gln Val Val Tyr
Ile Ser Tyr Leu 1370 1375 1380 Ile Leu Glu Ile Thr Tyr Tyr Met His
Arg Lys Ile Ile Asp Glu 1385 1390 1395 Val Ser Gly Gly Ala Asn Phe
Leu Ser Arg Leu Ile Ala Ala Ile 1400 1405 1410 Ile Glu Leu Asn Trp
Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu 1415 1420 1425 Lys Lys Leu
Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val 1430 1435 1440 Lys
His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu 1445 1450
1455 Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala
1460 1465 1470 Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr
Ile Cys 1475 1480 1485 Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro
Lys Cys Gly Arg 1490 1495 1500 Gln Gly Lys Pro Ile Thr Cys Gly Met
Thr Leu Ala Asp Phe Glu 1505 1510 1515 Glu Lys His Tyr Lys Lys Ile
Phe Ile Arg Glu Glu Ser Ser Cys 1520 1525 1530 Pro Val Pro Phe Asp
Pro Ser Cys His Cys Asn Tyr Phe Arg His 1535 1540 1545 Asp Gly Pro
Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr 1550 1555 1560 Ala
Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr 1565 1570
1575 Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly
1580 1585 1590 Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala
Val Cys 1595 1600 1605 Lys Lys Ile Thr Asn His Glu Lys Cys His Val
Asn Ile Met Asp 1610 1615 1620 Lys Leu Thr Ala Phe Phe Gly Ile Met
Pro Arg Gly Thr Thr Pro 1625 1630 1635 Arg Ala Pro Val Arg Phe Pro
Thr Ala Leu Leu Lys Val Arg Arg 1640 1645 1650 Gly Leu Glu Thr Gly
Trp Ala Tyr Thr His Gln Gly Gly Ile Ser 1655 1660 1665 Ser Val Asp
His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp 1670 1675 1680 Ser
Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met 1685 1690
1695 Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro
1700 1705 1710 Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val
Asn Ile 1715 1720 1725 Ser Gly Thr Lys Gly Ala Met Val His Leu
Gln Lys Thr Gly Gly 1730 1735 1740 Glu Phe Thr Cys Val Thr Ala Ser
Gly Thr Pro Ala Phe Phe Asp 1745 1750 1755 Leu Lys Asn Leu Lys Gly
Trp Ser Gly Leu Pro Ile Phe Glu Ala 1760 1765 1770 Ser Ser Gly Arg
Val Val Gly Arg Val Lys Val Gly Lys Asn Glu 1775 1780 1785 Asp Ser
Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser 1790 1795 1800
Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser 1805
1810 1815 Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly
Ala 1820 1825 1830 Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu
Glu Ile Gly 1835 1840 1845 Arg His Lys Arg Val Leu Val Leu Ile Pro
Leu Arg Ala Ala Ala 1850 1855 1860 Glu Ser Val Tyr Gln Tyr Met Arg
Val Lys Tyr Pro Ser Ile Ser 1865 1870 1875 Phe Asn Leu Arg Ile Gly
Asp Met Lys Glu Gly Asp Met Ala Thr 1880 1885 1890 Gly Ile Thr Tyr
Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln 1895 1900 1905 Pro Lys
Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu 1910 1915 1920
Asp Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly 1925
1930 1935 Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met
Thr 1940 1945 1950 Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln
Lys His Pro 1955 1960 1965 Ile Glu Glu Phe Ile Ala Pro Glu Val Met
Lys Gly Glu Asp Leu 1970 1975 1980 Gly Ser Glu Tyr Leu Asp Ile Ala
Gly Leu Lys Ile Pro Thr Glu 1985 1990 1995 Glu Met Lys Gly Asn Met
Leu Val Phe Ala Pro Thr Arg Asn Met 2000 2005 2010 Ala Val Glu Thr
Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser 2015 2020 2025 Gly Tyr
Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val 2030 2035 2040
Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu 2045
2050 2055 Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr
Gly 2060 2065 2070 Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys
Met Pro Phe 2075 2080 2085 Ile Val Thr Gly Leu Lys Arg Met Ala Val
Thr Ile Gly Glu Gln 2090 2095 2100 Ala Gln Arg Arg Gly Arg Val Gly
Arg Val Lys Pro Gly Arg Tyr 2105 2110 2115 Tyr Arg Ser Gln Glu Thr
Ala Ser Gly Ser Lys Asp Tyr His Tyr 2120 2125 2130 Asp Leu Leu Gln
Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn 2135 2140 2145 Val Thr
Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr 2150 2155 2160
Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn 2165
2170 2175 Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile
Met 2180 2185 2190 Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala
Tyr Asn Ser 2195 2200 2205 Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro
Lys Ile Lys Asn Gly 2210 2215 2220 Glu Val Thr Asp Ser Tyr Glu Asn
Tyr Thr Tyr Leu Asn Ala Arg 2225 2230 2235 Lys Leu Gly Glu Asp Val
Pro Ala Tyr Val Tyr Ala Thr Glu Asp 2240 2245 2250 Glu Asp Leu Ala
Val Asp Leu Leu Gly Met Asp Trp Pro Asp Pro 2255 2260 2265 Gly Asn
Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val 2270 2275 2280
Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe 2285
2290 2295 Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro
Met 2300 2305 2310 Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu
Glu Asp Thr 2315 2320 2325 Thr His Leu Gln Phe Ala Pro Asn Ala Ile
Arg Thr Asp Gly Lys 2330 2335 2340 Asp Ser Glu Leu Lys Glu Leu Ala
Val Gly Asp Leu Asp Lys Tyr 2345 2350 2355 Val Asp Ala Leu Val Asp
Tyr Ser Lys Gln Gly Met Lys Phe Ile 2360 2365 2370 Lys Val Gln Ala
Glu Lys Val Arg Asp Ser Gln Ser Thr Lys Glu 2375 2380 2385 Gly Leu
Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser 2390 2395 2400
Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly 2405
2410 2415 Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly
His 2420 2425 2430 Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu
Ala Phe Gly 2435 2440 2445 Gly Glu Thr Val Ser Ala His Ile Lys Gln
Val Ala Val Asp Leu 2450 2455 2460 Val Val Tyr Tyr Ile Ile Asn Lys
Pro Ser Phe Pro Gly Asp Thr 2465 2470 2475 Glu Thr Gln Gln Glu Gly
Arg Arg Phe Val Ala Ser Leu Phe Ile 2480 2485 2490 Ser Ala Leu Ala
Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn 2495 2500 2505 Leu Gln
Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala 2510 2515 2520
Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val 2525
2530 2535 Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile
Arg 2540 2545 2550 Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile
Ser Ala Ala 2555 2560 2565 Met Glu Ile Leu Asn Gln Asn Pro Ile Ser
Val Gly Ile Ser Val 2570 2575 2580 Met Leu Gly Val Gly Ala Ile Ala
Ala His Asn Ala Ile Glu Ser 2585 2590 2595 Ser Glu Gln Lys Arg Thr
Leu Leu Met Lys Val Phe Val Lys Asn 2600 2605 2610 Phe Leu Asp Gln
Ala Ala Thr Asp Glu Leu Val Lys Glu Asn Pro 2615 2620 2625 Glu Lys
Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly 2630 2635 2640
Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys 2645
2650 2655 Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg
Asn 2660 2665 2670 Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu
Leu Gly Met 2675 2680 2685 Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser
Gly Asn Tyr Ile Leu 2690 2695 2700 Asp Leu Ile Phe Asn Leu His Asn
Lys Leu Asn Lys Gly Leu Lys 2705 2710 2715 Lys Leu Val Leu Gly Trp
Ala Pro Ala Pro Leu Ser Cys Asp Trp 2720 2725 2730 Thr Pro Ser Asp
Glu Arg Ile Ser Leu Pro His Asn Asn Tyr Leu 2735 2740 2745 Arg Val
Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile 2750 2755 2760
Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser 2765
2770 2775 Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe
Lys 2780 2785 2790 Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val
Lys Pro Val 2795 2800 2805 Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr
Tyr Lys Gly Val Thr 2810 2815 2820 Ala Lys Leu Asp Tyr Asn Asn Gly
Lys Val Leu Leu Ala Thr Asn 2825 2830 2835 Lys Trp Glu Val Asp His
Ala Phe Leu Thr Arg Leu Val Lys Lys 2840 2845 2850 His Thr Gly Ile
Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro 2855 2860 2865 Asp His
Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys 2870 2875 2880
Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr 2885
2890 2895 Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu
Val 2900 2905 2910 His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr
Val Thr Val 2915 2920 2925 Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn
Glu Asp Leu Gly Thr 2930 2935 2940 Ile Lys Pro Val Leu Gly Glu Lys
Val Ile Pro Glu Pro Pro Glu 2945 2950 2955 Glu Leu Ser Leu Gln Pro
Thr Val Arg Leu Val Thr Thr Glu Thr 2960 2965 2970 Ala Ile Thr Ile
Thr Gly Glu Ala Glu Val Met Thr Thr Gly Ile 2975 2980 2985 Thr Pro
Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln 2990 2995 3000
Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro Gly 3005
3010 3015 Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu
Lys 3020 3025 3030 Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys
Gly Ser Thr 3035 3040 3045 Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile
Arg Leu Tyr Lys Gly 3050 3055 3060 Asn Asn Pro Arg Glu Ile Arg Asp
Leu Met Ser Gln Gly Arg Ile 3065 3070 3075 Leu Thr Val Ala Leu Lys
Glu Leu Asp Pro Glu Leu Lys Glu Leu 3080 3085 3090 Val Asp Tyr Lys
Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu Ala 3095 3100 3105 Leu Ser
Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met 3110 3115 3120
Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro Asp 3125
3130 3135 Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile
Gln 3140 3145 3150 Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser
Ile Lys Ser 3155 3160 3165 Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn
Thr Lys Ile Val Lys 3170 3175 3180 Glu Val Gly Ala Arg Thr Tyr Thr
Met Lys Leu Ser Ser Trp Ser 3185 3190 3195 Thr Gln Val Thr Lys Lys
Gln Met Ser Leu Ala Pro Leu Phe Glu 3200 3205 3210 Glu Leu Leu Leu
Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys Gly 3215 3220 3225 His Met
Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro 3230 3235 3240
Leu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg Leu 3245
3250 3255 Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu
Val 3260 3265 3270 Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser
Ile Ile Arg 3275 3280 3285 Glu His Asn Thr Trp Ile Leu Arg Lys Val
Arg His Glu Gly Asn 3290 3295 3300 Leu Arg Thr Lys Ser Met Ile Asn
Pro Gly Lys Ile Ser Asp Gln 3305 3310 3315 Leu Cys Arg Asp Gly His
Lys Arg Asn Ile Tyr Asn Lys Ile Ile 3320 3325 3330 Gly Ser Thr Met
Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu Pro 3335 3340 3345 Val Val
Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile 3350 3355 3360
Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu Leu 3365
3370 3375 His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro
Glu 3380 3385 3390 Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln
Leu Glu Ala 3395 3400 3405 Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr
Leu Glu Ser Lys Asn 3410 3415 3420 Ile Gly Glu Val Leu Asp Thr Glu
Lys His Ile Val Glu Gln Leu 3425 3430 3435 Ile Lys Asp Leu Arg Lys
Gly Lys Lys Ile Arg Tyr Tyr Glu Thr 3440 3445 3450 Ala Ile Pro Lys
Asn Glu Lys Arg Asp Val Ser Asp Asp Trp Glu 3455 3460 3465 Ala Gly
Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr 3470 3475 3480
Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr Lys 3485
3490 3495 Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly
Lys 3500 3505 3510 Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys
Glu Trp Asp 3515 3520 3525 Ser Phe Gln Asp Pro Val Ala Val Ser Phe
Asp Thr Lys Ala Trp 3530 3535 3540 Asp Thr Gln Val Thr Ser Arg Asp
Leu Met Leu Ile Lys Asp Ile 3545 3550 3555 Gln Lys Tyr Tyr Phe Lys
Arg Ser Ile His Lys Phe Leu Asp Thr 3560 3565 3570 Ile Thr Glu His
Met Val Glu Val Pro Val Ile Thr Ala Asp Gly 3575 3580 3585 Glu Val
Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp 3590 3595 3600
Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile Tyr 3605
3610 3615 Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser
Arg 3620 3625 3630 Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe
Leu Ile Thr 3635 3640 3645 Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu
Lys Gly Met Gln Ile 3650 3655 3660 Leu His Glu Ala Gly Lys Pro Gln
Lys Ile Thr Glu Gly Asp Lys 3665 3670 3675 Met Lys Val Ala Tyr Arg
Phe Glu Asp Ile Glu Phe Cys Ser His 3680 3685 3690 Thr Pro Val Pro
Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr Met 3695 3700 3705 Ala Gly
Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr Arg 3710 3715 3720
Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys Ala 3725
3730 3735 Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val
Val 3740 3745 3750 Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro
Glu Ile Ser 3755 3760 3765 Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln
Gly Asp Pro Ile Ala 3770 3775 3780 Ala Tyr Arg Glu Val Ile Gly Lys
Gln Leu Cys Glu Leu Lys Arg 3785 3790 3795 Thr Gly Phe Glu Lys Leu
Ala Gly Leu Asn Leu Ser Met Thr Thr 3800 3805 3810 Leu Gly Ile Trp
Thr Lys His Thr Ser Lys Arg Leu Ile Gln Ala 3815 3820 3825 Cys Val
Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn Ala 3830 3835 3840
Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro Ser 3845
3850 3855 Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn
Leu 3860 3865 3870 Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val
Asp Arg Tyr 3875 3880 3885 Lys Leu Gly Pro Ile Val Asn Val Ile Leu
Arg Arg Leu Arg Val 3890 3895 3900 Met Leu Met Thr Val Ala Ser Gly
Ser Trp 3905 3910 63749PRTArtificial SequenceMutated BVDV
XIKE-A-NdN 6Met Glu Leu Phe Ser Asp Glu Gly Ser Lys Gly Ala Thr Ser
Lys Lys 1 5
10 15 Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met Lys Ile Ala
Pro 20 25 30 Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro Asp
Ala Thr Ile 35 40 45 Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys
Lys Gly Lys Val Arg 50 55 60 Gly Lys Asn Thr Gln Asp Gly Leu Tyr
His Asn Lys Asn Lys Pro Pro 65 70 75 80 Glu Ser Arg Lys Lys Leu Glu
Lys Ala Leu Leu Ala Trp Ala Ile Leu 85 90 95 Ala Ala Val Leu Leu
Gln Leu Val Thr Gly Glu Asn Ile Thr Gln Trp 100 105 110 Asn Leu Met
Asp Asn Gly Thr Glu Gly Ile Gln Gln Ala Met Phe Leu 115 120 125 Arg
Gly Val Asn Arg Ser Leu His Gly Ile Trp Pro Glu Lys Ile Cys 130 135
140 Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu Leu Lys Glu Ile
145 150 155 160 Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr Thr
Cys Cys Arg 165 170 175 Leu Gln Arg His Glu Trp Asn Lys His Gly Trp
Cys Asn Trp Phe His 180 185 190 Ile Glu Pro Trp Ile Trp Leu Met Asn
Lys Thr Gln Asn Asn Leu Thr 195 200 205 Glu Gly Gln Pro Leu Arg Glu
Cys Ala Val Thr Cys Arg Tyr Asp Lys 210 215 220 Glu Thr Glu Leu Asn
Ile Val Thr Gln Ala Arg Asp Arg Pro Thr Thr 225 230 235 240 Leu Thr
Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe Ala Gly Val Ile 245 250 255
Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu Asp Val Leu Phe 260
265 270 Lys Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr Ala Ile Gln
Leu 275 280 285 Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg Val
Gly Thr Ala 290 295 300 Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly
Ile Leu Gly Lys Lys 305 310 315 320 Leu Glu Asn Lys Ser Lys Ala Trp
Phe Gly Ala His Ala Ala Ser Pro 325 330 335 Tyr Cys Gly Val Glu Arg
Lys Ile Gly Tyr Val Trp Tyr Thr Lys Asn 340 345 350 Cys Thr Pro Ala
Cys Leu Pro Arg Asn Thr Arg Ile Ile Gly Pro Gly 355 360 365 Lys Phe
Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu His Glu Met Gly 370 375 380
Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu Val Val Leu Ser Asp 385
390 395 400 Phe Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val Leu His
Phe Ala 405 410 415 Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp
Lys Asn Gln Leu 420 425 430 Asn Leu Thr Val Ala Thr Thr Val Ala Glu
Val Ile Pro Gly Thr Val 435 440 445 Trp Asn Leu Gly Lys Tyr Val Cys
Ile Arg Pro Asp Trp Trp Pro Tyr 450 455 460 Glu Thr Thr Thr Val Phe
Val Ile Glu Glu Ala Gly Gln Val Ile Lys 465 470 475 480 Leu Met Leu
Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp Asn Ala Ala 485 490 495 Thr
Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala Leu Arg Gly Gln 500 505
510 Leu Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr Gly Ala Gln Gly
515 520 525 Phe Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile Ser Lys
Asp Arg 530 535 540 Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr
Thr Trp His Leu 545 550 555 560 Pro Thr Lys Lys Ile Val Asp Ser Met
Val His Val Trp Cys Glu Gly 565 570 575 Lys Asp Leu Lys Ile Leu Lys
Met Cys Thr Lys Glu Glu Arg Tyr Leu 580 585 590 Val Ala Val His Glu
Arg Ala Leu Ser Thr Ser Ala Glu Phe Met Gln 595 600 605 Ile Ser Asp
Gly Thr Ile Gly Pro Asp Val Ile Asp Met Pro Asp Asp 610 615 620 Phe
Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro Val Ile Lys Gly 625 630
635 640 Lys Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln Met Val
Cys 645 650 655 Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala
Asn Gln Asp 660 665 670 Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg
Arg Thr Thr Pro Phe 675 680 685 Gln Arg Arg Lys Trp Cys Thr Tyr Glu
Lys Ile Ile Gly Glu Asp Ile 690 695 700 Tyr Glu Cys Ile Leu Gly Gly
Asn Trp Thr Cys Ile Thr Gly Asp His 705 710 715 720 Ser Arg Leu Lys
Asp Gly Pro Ile Lys Lys Cys Lys Trp Cys Gly His 725 730 735 Asp Phe
Val Asn Ser Glu Gly Leu Pro His Tyr Pro Ile Gly Lys Cys 740 745 750
Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp Asp Thr Ser Cys 755
760 765 Asp Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr Val Lys Cys
Arg 770 775 780 Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn
Asp Leu Gly 785 790 795 800 Pro Met Pro Cys Ser Pro Ala Glu Val Ile
Ala Ser Glu Gly Pro Val 805 810 815 Glu Lys Thr Ala Cys Thr Phe Asn
Tyr Ser Arg Thr Leu Pro Asn Lys 820 825 830 Tyr Tyr Glu Pro Arg Asp
Arg Tyr Phe Gln Gln Tyr Met Leu Lys Gly 835 840 845 Glu Trp Gln Tyr
Trp Phe Asp Leu Asp Ser Val Asp His His Lys Asp 850 855 860 Tyr Phe
Ser Glu Phe Ile Ile Ile Ala Val Val Ala Leu Leu Gly Gly 865 870 875
880 Lys Tyr Val Leu Trp Leu Leu Ile Thr Tyr Thr Ile Leu Ser Glu Gln
885 890 895 Met Ala Met Gly Ala Gly Val Asn Thr Glu Glu Ile Val Met
Ile Gly 900 905 910 Asn Leu Leu Thr Asp Ser Asp Ile Glu Val Val Val
Tyr Phe Leu Leu 915 920 925 Leu Tyr Leu Ile Val Lys Glu Glu Leu Ala
Arg Lys Trp Ile Ile Leu 930 935 940 Val Tyr His Ile Leu Val Ala Asn
Pro Met Lys Thr Ile Gly Val Val 945 950 955 960 Leu Leu Met Leu Gly
Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp 965 970 975 Asp Gln Ser
Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val 980 985 990 Ala
Val Leu Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile 995
1000 1005 Val Ala Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro
Ala 1010 1015 1020 Ser Ile Asp Val Thr Val Ala Val Val Leu Ile Val
Leu Leu Leu 1025 1030 1035 Ala Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr
Lys Lys Trp Leu Gln 1040 1045 1050 Leu Leu Phe Ser Leu Ile Ala Gly
Ile Phe Ile Ile Arg Ser Leu 1055 1060 1065 Lys His Ile Asn Gln Met
Glu Val Pro Glu Ile Ser Met Pro Ser 1070 1075 1080 Trp Arg Pro Leu
Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala 1085 1090 1095 Ile Thr
Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln 1100 1105 1110
Trp Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe 1115
1120 1125 Leu Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys
Leu 1130 1135 1140 Tyr Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys
Asn Trp Leu 1145 1150 1155 Gly Lys Val Lys Tyr Arg Gln Ile Ser Ser
Val Tyr Asp Ile Cys 1160 1165 1170 Asp Ser Glu Glu Ala Val Tyr Leu
Phe Pro Ser Arg His Lys Ser 1175 1180 1185 Gly Ser Arg Pro Asp Phe
Ile Leu Pro Phe Leu Lys Ala Val Leu 1190 1195 1200 Ile Ser Cys Ile
Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr 1205 1210 1215 Leu Ile
Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp 1220 1225 1230
Glu Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala 1235
1240 1245 Ile Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys
Gly 1250 1255 1260 Leu Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys
Asn Leu Ile 1265 1270 1275 Val Lys His Lys Val Arg Asn Glu Ala Val
His Arg Trp Phe Gly 1280 1285 1290 Glu Glu Glu Ile Tyr Gly Ala Pro
Lys Val Ile Thr Ile Ile Lys 1295 1300 1305 Ala Ser Thr Leu Ser Lys
Asn Arg His Cys Ile Ile Cys Thr Ile 1310 1315 1320 Cys Glu Gly Lys
Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly 1325 1330 1335 Arg Gln
Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe 1340 1345 1350
Glu Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser 1355
1360 1365 Cys Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe
Arg 1370 1375 1380 His Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr
Val Gln Tyr 1385 1390 1395 Thr Ala Arg Gly Gln Leu Phe Leu Arg Asn
Leu Pro Ile Leu Ala 1400 1405 1410 Thr Lys Met Lys Leu Leu Met Val
Gly Asn Leu Gly Ala Glu Ile 1415 1420 1425 Gly Asp Leu Glu His Leu
Gly Trp Val Leu Arg Gly Pro Ala Val 1430 1435 1440 Cys Lys Lys Ile
Thr Asn His Glu Lys Cys His Val Asn Ile Met 1445 1450 1455 Asp Lys
Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr 1460 1465 1470
Pro Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg 1475
1480 1485 Arg Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly
Ile 1490 1495 1500 Ser Ser Val Asp His Val Thr Ala Gly Lys Asp Leu
Leu Val Cys 1505 1510 1515 Asp Ser Met Gly Arg Thr Arg Val Val Cys
His Ser Asn Asn Lys 1520 1525 1530 Met Thr Asp Glu Thr Glu Tyr Gly
Ile Lys Thr Asp Ser Gly Cys 1535 1540 1545 Pro Glu Gly Ala Arg Cys
Tyr Val Leu Asn Pro Glu Ala Val Asn 1550 1555 1560 Ile Ser Gly Thr
Lys Gly Ala Met Val His Leu Gln Lys Thr Gly 1565 1570 1575 Gly Glu
Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe 1580 1585 1590
Asp Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu 1595
1600 1605 Ala Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys
Asn 1610 1615 1620 Glu Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile
Gln Thr Val 1625 1630 1635 Ser Lys Asn Gln Thr Asp Leu Ala Asp Ile
Val Lys Lys Leu Thr 1640 1645 1650 Ser Met Asn Arg Gly Glu Phe Lys
Gln Ile Thr Leu Ala Thr Gly 1655 1660 1665 Ala Gly Lys Thr Thr Glu
Leu Pro Arg Ser Val Ile Glu Glu Ile 1670 1675 1680 Gly Arg His Lys
Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala 1685 1690 1695 Ala Glu
Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile 1700 1705 1710
Ser Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala 1715
1720 1725 Thr Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu
Pro 1730 1735 1740 Gln Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser
Tyr Ile Phe 1745 1750 1755 Leu Asp Glu Tyr His Cys Ala Thr Pro Glu
Gln Leu Ala Ile Ile 1760 1765 1770 Gly Lys Ile His Arg Phe Ala Glu
Asn Leu Arg Val Val Ala Met 1775 1780 1785 Thr Ala Thr Pro Ala Gly
Thr Val Thr Thr Thr Gly Gln Lys His 1790 1795 1800 Pro Ile Glu Glu
Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp 1805 1810 1815 Leu Gly
Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr 1820 1825 1830
Glu Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn 1835
1840 1845 Met Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr
Asn 1850 1855 1860 Ser Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn
Leu Arg Val 1865 1870 1875 Val Thr Ser Gln Ser Pro Tyr Val Val Val
Ala Thr Asn Ala Ile 1880 1885 1890 Glu Ser Gly Val Thr Leu Pro Asp
Leu Asp Thr Val Val Asp Thr 1895 1900 1905 Gly Leu Lys Cys Glu Lys
Arg Val Arg Ile Ser Ser Lys Met Pro 1910 1915 1920 Phe Ile Val Thr
Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu 1925 1930 1935 Gln Ala
Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg 1940 1945 1950
Tyr Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His 1955
1960 1965 Tyr Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly
Ile 1970 1975 1980 Asn Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp
Trp Ser Leu 1985 1990 1995 Tyr Glu Glu Asp Ser Leu Met Ile Thr Gln
Leu Glu Val Leu Asn 2000 2005 2010 Asn Leu Leu Ile Ser Glu Asp Leu
Pro Ala Ala Val Lys Asn Ile 2015 2020 2025 Met Ala Arg Thr Asp His
Pro Glu Pro Ile Gln Leu Ala Tyr Asn 2030 2035 2040 Ser Tyr Glu Asn
Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn 2045 2050 2055 Gly Glu
Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala 2060 2065 2070
Arg Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu 2075
2080 2085 Asp Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro
Asp 2090 2095 2100 Pro Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala
Leu Lys Gln 2105 2110 2115 Val Thr Gly Leu Ser Thr Ala Glu Asn Ala
Leu Leu Ile Ala Leu 2120 2125 2130 Phe Gly Tyr Val Gly Tyr Gln Thr
Leu Ser Lys Arg His Ile Pro 2135 2140 2145 Met Ile Thr Asp Ile Tyr
Thr Leu Glu Asp His Arg Leu Glu Asp 2150 2155 2160 Thr Thr His Leu
Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly 2165 2170 2175 Lys Asp
Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys 2180 2185 2190
Tyr Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe 2195
2200 2205 Ile Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr
Lys 2210 2215 2220 Glu Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys
Phe Ile Gln 2225 2230 2235 Ser Leu Thr Glu Asn Lys Glu Glu Ile Ile
Arg Tyr Gly Leu Trp 2240 2245 2250
Gly Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly 2255
2260 2265 His Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala
Phe 2270 2275 2280 Gly Gly Glu Thr Val Ser Ala His Ile Lys Gln Val
Ala Val Asp 2285 2290 2295 Leu Val Val Tyr Tyr Ile Ile Asn Lys Pro
Ser Phe Pro Gly Asp 2300 2305 2310 Thr Glu Thr Gln Gln Glu Gly Arg
Arg Phe Val Ala Ser Leu Phe 2315 2320 2325 Ile Ser Ala Leu Ala Thr
Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn 2330 2335 2340 Asn Leu Gln Arg
Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr 2345 2350 2355 Ala Thr
Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser 2360 2365 2370
Val Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile 2375
2380 2385 Arg Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser
Ala 2390 2395 2400 Ala Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val
Gly Ile Ser 2405 2410 2415 Val Met Leu Gly Val Gly Ala Ile Ala Ala
His Asn Ala Ile Glu 2420 2425 2430 Ser Ser Glu Gln Lys Arg Thr Leu
Leu Met Lys Val Phe Val Lys 2435 2440 2445 Asn Phe Leu Asp Gln Ala
Ala Thr Asp Glu Leu Val Lys Glu Asn 2450 2455 2460 Pro Glu Lys Ile
Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile 2465 2470 2475 Gly Asn
Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr 2480 2485 2490
Lys Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg 2495
2500 2505 Asn Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu
Gly 2510 2515 2520 Met Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly
Asn Tyr Ile 2525 2530 2535 Leu Asp Leu Ile Phe Asn Leu His Asn Lys
Leu Asn Lys Gly Leu 2540 2545 2550 Lys Lys Leu Val Leu Gly Trp Ala
Pro Ala Pro Leu Ser Cys Asp 2555 2560 2565 Trp Thr Pro Ser Asp Glu
Arg Ile Ser Leu Pro His Asn Asn Tyr 2570 2575 2580 Leu Arg Val Glu
Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala 2585 2590 2595 Ile Lys
Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly 2600 2605 2610
Ser Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe 2615
2620 2625 Lys Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys
Pro 2630 2635 2640 Val Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr
Lys Gly Val 2645 2650 2655 Thr Ala Lys Leu Asp Tyr Asn Asn Gly Lys
Val Leu Leu Ala Thr 2660 2665 2670 Asn Lys Trp Glu Val Asp His Ala
Phe Leu Thr Arg Leu Val Lys 2675 2680 2685 Lys His Thr Gly Ile Gly
Phe Lys Gly Ala Tyr Leu Gly Asp Arg 2690 2695 2700 Pro Asp His Gln
Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr 2705 2710 2715 Lys Asn
Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe 2720 2725 2730
Thr Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu 2735
2740 2745 Val His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val
Thr 2750 2755 2760 Val Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu
Asp Leu Gly 2765 2770 2775 Thr Ile Lys Pro Val Leu Gly Glu Lys Val
Ile Pro Glu Pro Pro 2780 2785 2790 Glu Glu Leu Ser Leu Gln Pro Thr
Val Arg Leu Val Thr Thr Glu 2795 2800 2805 Thr Ala Ile Thr Ile Thr
Gly Glu Ala Glu Val Met Thr Thr Gly 2810 2815 2820 Ile Thr Pro Val
Val Glu Met Lys Glu Glu Pro Gln Leu Asp His 2825 2830 2835 Gln Ser
Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro 2840 2845 2850
Gly Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu 2855
2860 2865 Lys Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly
Ser 2870 2875 2880 Thr Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg
Leu Tyr Lys 2885 2890 2895 Gly Asn Asn Pro Arg Glu Ile Arg Asp Leu
Met Ser Gln Gly Arg 2900 2905 2910 Ile Leu Thr Val Ala Leu Lys Glu
Leu Asp Pro Glu Leu Lys Glu 2915 2920 2925 Leu Val Asp Tyr Lys Gly
Thr Phe Leu Asn Arg Glu Ala Leu Glu 2930 2935 2940 Ala Leu Ser Leu
Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala 2945 2950 2955 Met Ile
Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro 2960 2965 2970
Asp Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile 2975
2980 2985 Gln Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile
Lys 2990 2995 3000 Ser Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr
Lys Ile Val 3005 3010 3015 Lys Glu Val Gly Ala Arg Thr Tyr Thr Met
Lys Leu Ser Ser Trp 3020 3025 3030 Ser Thr Gln Val Thr Lys Lys Gln
Met Ser Leu Ala Pro Leu Phe 3035 3040 3045 Glu Glu Leu Leu Leu Lys
Cys Pro Pro Cys Ser Lys Ile Ser Lys 3050 3055 3060 Gly His Met Val
Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu 3065 3070 3075 Pro Leu
Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg 3080 3085 3090
Leu Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu 3095
3100 3105 Val Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile
Ile 3110 3115 3120 Arg Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg
His Glu Gly 3125 3130 3135 Asn Leu Arg Thr Lys Ser Met Ile Asn Pro
Gly Lys Ile Ser Asp 3140 3145 3150 Gln Leu Cys Arg Asp Gly His Lys
Arg Asn Ile Tyr Asn Lys Ile 3155 3160 3165 Ile Gly Ser Thr Met Ala
Ser Ala Gly Ile Arg Leu Glu Lys Leu 3170 3175 3180 Pro Val Val Arg
Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala 3185 3190 3195 Ile Arg
Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu 3200 3205 3210
Leu His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro 3215
3220 3225 Glu Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu
Glu 3230 3235 3240 Ala Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu
Glu Ser Lys 3245 3250 3255 Asn Ile Gly Glu Val Leu Asp Thr Glu Lys
His Ile Val Glu Gln 3260 3265 3270 Leu Ile Lys Asp Leu Arg Lys Gly
Lys Lys Ile Arg Tyr Tyr Glu 3275 3280 3285 Thr Ala Ile Pro Lys Asn
Glu Lys Arg Asp Val Ser Asp Asp Trp 3290 3295 3300 Glu Ala Gly Glu
Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln 3305 3310 3315 Tyr Pro
Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr 3320 3325 3330
Lys Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly 3335
3340 3345 Lys Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu
Trp 3350 3355 3360 Asp Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp
Thr Lys Ala 3365 3370 3375 Trp Asp Thr Gln Val Thr Ser Arg Asp Leu
Met Leu Ile Lys Asp 3380 3385 3390 Ile Gln Lys Tyr Tyr Phe Lys Arg
Ser Ile His Lys Phe Leu Asp 3395 3400 3405 Thr Ile Thr Glu His Met
Val Glu Val Pro Val Ile Thr Ala Asp 3410 3415 3420 Gly Glu Val Tyr
Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro 3425 3430 3435 Asp Thr
Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile 3440 3445 3450
Tyr Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser 3455
3460 3465 Arg Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu
Ile 3470 3475 3480 Thr Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys
Gly Met Gln 3485 3490 3495 Ile Leu His Glu Ala Gly Lys Pro Gln Lys
Ile Thr Glu Gly Asp 3500 3505 3510 Lys Met Lys Val Ala Tyr Arg Phe
Glu Asp Ile Glu Phe Cys Ser 3515 3520 3525 His Thr Pro Val Pro Val
Arg Trp Ala Asp Asn Thr Ser Ser Tyr 3530 3535 3540 Met Ala Gly Arg
Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr 3545 3550 3555 Arg Leu
Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys 3560 3565 3570
Ala Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val 3575
3580 3585 Val Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu
Ile 3590 3595 3600 Ser Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly
Asp Pro Ile 3605 3610 3615 Ala Ala Tyr Arg Glu Val Ile Gly Lys Gln
Leu Cys Glu Leu Lys 3620 3625 3630 Arg Thr Gly Phe Glu Lys Leu Ala
Gly Leu Asn Leu Ser Met Thr 3635 3640 3645 Thr Leu Gly Ile Trp Thr
Lys His Thr Ser Lys Arg Leu Ile Gln 3650 3655 3660 Ala Cys Val Glu
Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn 3665 3670 3675 Ala Asp
Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro 3680 3685 3690
Ser Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn 3695
3700 3705 Leu Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp
Arg 3710 3715 3720 Tyr Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg
Arg Leu Arg 3725 3730 3735 Val Met Leu Met Thr Val Ala Ser Gly Ser
Trp 3740 3745 73912PRTArtificial SequenceMutated BVDV XIKE-B 7Met
Glu Leu Phe Ser Asn Glu Leu Leu Tyr Lys Thr Tyr Lys Gln Lys 1 5 10
15 Pro Ala Gly Val Val Glu Pro Val Tyr Asp Val Asn Gly Arg Pro Leu
20 25 30 Phe Gly Glu Ser Ser Asp Leu His Pro Gln Ser Thr Leu Lys
Leu Pro 35 40 45 His Gln Arg Gly Ser Ala Asn Ile Leu Thr Asn Ala
Arg Ser Leu Pro 50 55 60 Arg Lys Gly Asp Cys Arg Arg Gly Asn Val
Tyr Gly Pro Val Ser Gly 65 70 75 80 Ile Tyr Ile Lys Pro Gly Pro Ile
Tyr Tyr Gln Asp Tyr Val Gly Pro 85 90 95 Val Tyr His Arg Ala Pro
Leu Glu Leu Cys Arg Glu Ala Ser Met Cys 100 105 110 Glu Thr Thr Arg
Arg Val Gly Arg Val Thr Gly Ser Asp Gly Lys Leu 115 120 125 Tyr His
Ile Tyr Ile Cys Ile Asp Gly Cys Ile Leu Leu Lys Arg Ala 130 135 140
Thr Arg Asn Gln Pro Glu Val Leu Lys Trp Val Tyr Asn Arg Leu Asn 145
150 155 160 Cys Pro Leu Trp Val Thr Ser Cys Ser Asp Glu Gly Ser Lys
Gly Ala 165 170 175 Thr Ser Lys Lys Gln Pro Lys Pro Asp Arg Ile Glu
Lys Gly Lys Met 180 185 190 Lys Ile Ala Pro Lys Glu Thr Glu Lys Asp
Cys Lys Thr Arg Pro Pro 195 200 205 Asp Ala Thr Ile Val Val Glu Gly
Val Lys Tyr Gln Val Lys Lys Lys 210 215 220 Gly Lys Val Arg Gly Lys
Asn Thr Gln Asp Gly Leu Tyr His Asn Lys 225 230 235 240 Asn Lys Pro
Pro Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala 245 250 255 Trp
Ala Ile Leu Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn 260 265
270 Ile Thr Gln Trp Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln
275 280 285 Ala Met Phe Leu Arg Gly Val Asn Arg Ser Leu His Gly Ile
Trp Pro 290 295 300 Glu Lys Ile Cys Thr Gly Val Pro Thr His Leu Ala
Thr Asp Tyr Glu 305 310 315 320 Leu Lys Glu Ile Val Gly Met Met Asp
Ala Ser Glu Lys Thr Asn Tyr 325 330 335 Thr Cys Cys Arg Leu Gln Arg
His Glu Trp Asn Lys Gly Trp Cys Asn 340 345 350 Trp Phe His Ile Glu
Pro Trp Ile Trp Leu Met Asn Lys Thr Gln Asn 355 360 365 Asn Leu Thr
Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys Arg 370 375 380 Tyr
Asp Lys Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp Arg 385 390
395 400 Pro Thr Thr Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe
Ala 405 410 415 Gly Val Ile Leu Asp Gly Pro Cys Asn Phe Lys Val Ser
Val Glu Asp 420 425 430 Val Leu Phe Lys Glu His Asp Cys Gly Asn Met
Leu Gln Glu Thr Ala 435 440 445 Ile Gln Leu Leu Asp Gly Ala Thr Asn
Thr Ile Glu Gly Ala Arg Val 450 455 460 Gly Thr Ala Lys Leu Thr Thr
Trp Leu Gly Lys Gln Leu Gly Ile Leu 465 470 475 480 Gly Lys Lys Leu
Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala 485 490 495 Ala Ser
Pro Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp Tyr 500 505 510
Thr Lys Asn Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile Ile 515
520 525 Gly Pro Gly Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu
His 530 535 540 Glu Met Gly Gly His Leu Ser Glu Phe Val Leu Leu Ser
Leu Val Val 545 550 555 560 Leu Ser Asp Phe Ala Pro Glu Thr Ala Ser
Val Ile Tyr Leu Val Leu 565 570 575 His Phe Ala Ile Pro Gln Ser His
Val Asp Val Asp Thr Cys Asp Lys 580 585 590 Asn Gln Leu Asn Leu Thr
Val Ala Thr Thr Val Ala Glu Val Ile Pro 595 600 605 Gly Thr Val Trp
Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp 610 615 620 Trp Pro
Tyr Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly Gln 625 630 635
640 Val Ile Lys Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp
645 650 655 Asn Ala Ala Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys
Ala Leu 660 665 670 Arg Gly Gln Leu Ile Gln Gly Leu Leu Trp Leu Met
Leu Ile Thr Gly 675 680 685 Ala Gln Gly Phe Pro Glu Cys Lys Glu Gly
Phe Gln Tyr Ala Ile Ser 690 695 700 Lys Asp Arg Lys Met Gly Leu Leu
Gly Pro Glu Ser Leu Thr Thr Thr 705 710 715 720 Trp His Leu Pro Thr
Lys Lys Ile Val Asp Ser Met Val His Val Trp
725 730 735 Cys Glu Gly Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys
Glu Glu 740 745 750 Arg Tyr Leu Val Ala Val His Glu Arg Ala Leu Ser
Thr Ser Ala Glu 755 760 765 Phe Met Gln Ile Ser Asp Gly Thr Ile Gly
Pro Asp Val Ile Asp Met 770 775 780 Pro Asp Asp Phe Glu Phe Gly Leu
Cys Pro Cys Asp Ser Lys Pro Val 785 790 795 800 Ile Lys Gly Lys Phe
Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln 805 810 815 Met Val Cys
Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala 820 825 830 Asn
Gln Asp Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg Thr 835 840
845 Thr Pro Phe Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly
850 855 860 Glu Asp Ile Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys
Ile Thr 865 870 875 880 Gly Asp His Ser Arg Leu Lys Asp Gly Pro Ile
Lys Lys Cys Lys Trp 885 890 895 Cys Gly His Asp Phe Val Asn Ser Glu
Gly Leu Pro His Tyr Pro Ile 900 905 910 Gly Lys Cys Met Leu Ile Asn
Glu Ser Gly Tyr Arg Tyr Val Asp Asp 915 920 925 Thr Ser Cys Asp Arg
Gly Gly Val Ala Ile Val Pro Ser Gly Thr Val 930 935 940 Lys Cys Arg
Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn 945 950 955 960
Asp Leu Gly Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser Glu 965
970 975 Gly Pro Val Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr
Leu 980 985 990 Pro Asn Lys Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln
Gln Tyr Met 995 1000 1005 Leu Lys Gly Glu Trp Gln Tyr Trp Phe Asp
Leu Asp Ser Val Asp 1010 1015 1020 His His Lys Asp Tyr Phe Ser Glu
Phe Ile Ile Ile Ala Val Val 1025 1030 1035 Ala Leu Leu Gly Gly Lys
Tyr Val Leu Trp Leu Leu Ile Thr Tyr 1040 1045 1050 Thr Ile Leu Ser
Glu Gln Met Ala Met Gly Ala Gly Val Asn Thr 1055 1060 1065 Glu Glu
Ile Val Met Ile Gly Asn Leu Leu Thr Asp Ser Asp Ile 1070 1075 1080
Glu Val Val Val Tyr Phe Leu Leu Leu Tyr Leu Ile Val Lys Glu 1085
1090 1095 Glu Leu Ala Arg Lys Trp Ile Ile Leu Val Tyr His Ile Leu
Val 1100 1105 1110 Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu Leu
Met Leu Gly 1115 1120 1125 Gly Val Val Lys Ala Ser Arg Ile Asn Ala
Asp Asp Gln Ser Ala 1130 1135 1140 Met Asp Pro Cys Phe Leu Leu Val
Thr Gly Val Val Ala Val Leu 1145 1150 1155 Met Ile Ala Arg Arg Glu
Pro Ala Thr Leu Pro Leu Ile Val Ala 1160 1165 1170 Leu Leu Ala Ile
Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser Ile 1175 1180 1185 Asp Val
Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala Ser 1190 1195 1200
Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu Leu 1205
1210 1215 Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys
His 1220 1225 1230 Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro
Ser Trp Arg 1235 1240 1245 Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr
Ser Thr Ala Ile Thr 1250 1255 1260 Thr Asn Trp Asp Ile Asp Leu Ala
Gly Phe Leu Leu Gln Trp Ala 1265 1270 1275 Pro Ala Val Ile Met Met
Ala Thr Met Trp Ala Asp Phe Leu Thr 1280 1285 1290 Leu Ile Ile Val
Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr Phe 1295 1300 1305 Leu Lys
Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly Lys 1310 1315 1320
Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp Ser 1325
1330 1335 Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly
Ser 1340 1345 1350 Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val
Leu Ile Ser 1355 1360 1365 Cys Ile Ser Ser Gln Trp Gln Val Val Tyr
Ile Ser Tyr Leu Ile 1370 1375 1380 Leu Glu Ile Thr Tyr Tyr Met His
Arg Lys Ile Ile Asp Glu Val 1385 1390 1395 Ser Gly Gly Ala Asn Phe
Leu Ser Arg Leu Ile Ala Ala Ile Ile 1400 1405 1410 Glu Leu Asn Trp
Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu Lys 1415 1420 1425 Lys Leu
Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val Lys 1430 1435 1440
His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu Glu 1445
1450 1455 Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala
Ser 1460 1465 1470 Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr
Ile Cys Glu 1475 1480 1485 Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro
Lys Cys Gly Arg Gln 1490 1495 1500 Gly Lys Pro Ile Thr Cys Gly Met
Thr Leu Ala Asp Phe Glu Glu 1505 1510 1515 Lys His Tyr Lys Lys Ile
Phe Ile Arg Glu Glu Ser Ser Cys Pro 1520 1525 1530 Val Pro Phe Asp
Pro Ser Cys His Cys Asn Tyr Phe Arg His Asp 1535 1540 1545 Gly Pro
Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr Ala 1550 1555 1560
Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr Lys 1565
1570 1575 Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly
Asp 1580 1585 1590 Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala
Val Cys Lys 1595 1600 1605 Lys Ile Thr Asn His Glu Lys Cys His Val
Asn Ile Met Asp Lys 1610 1615 1620 Leu Thr Ala Phe Phe Gly Ile Met
Pro Arg Gly Thr Thr Pro Arg 1625 1630 1635 Ala Pro Val Arg Phe Pro
Thr Ala Leu Leu Lys Val Arg Arg Gly 1640 1645 1650 Leu Glu Thr Gly
Trp Ala Tyr Thr His Gln Gly Gly Ile Ser Ser 1655 1660 1665 Val Asp
His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp Ser 1670 1675 1680
Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met Thr 1685
1690 1695 Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro
Glu 1700 1705 1710 Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val
Asn Ile Ser 1715 1720 1725 Gly Thr Lys Gly Ala Met Val His Leu Gln
Lys Thr Gly Gly Glu 1730 1735 1740 Phe Thr Cys Val Thr Ala Ser Gly
Thr Pro Ala Phe Phe Asp Leu 1745 1750 1755 Lys Asn Leu Lys Gly Trp
Ser Gly Leu Pro Ile Phe Glu Ala Ser 1760 1765 1770 Ser Gly Arg Val
Val Gly Arg Val Lys Val Gly Lys Asn Glu Asp 1775 1780 1785 Ser Lys
Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser Lys 1790 1795 1800
Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser Met 1805
1810 1815 Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly Ala
Gly 1820 1825 1830 Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu
Ile Gly Arg 1835 1840 1845 His Lys Arg Val Leu Val Leu Ile Pro Leu
Arg Ala Ala Ala Glu 1850 1855 1860 Ser Val Tyr Gln Tyr Met Arg Val
Lys Tyr Pro Ser Ile Ser Phe 1865 1870 1875 Asn Leu Arg Ile Gly Asp
Met Lys Glu Gly Asp Met Ala Thr Gly 1880 1885 1890 Ile Thr Tyr Ala
Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln Pro 1895 1900 1905 Lys Leu
Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu Asp 1910 1915 1920
Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly Lys 1925
1930 1935 Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met Thr
Ala 1940 1945 1950 Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys
His Pro Ile 1955 1960 1965 Glu Glu Phe Ile Ala Pro Glu Val Met Lys
Gly Glu Asp Leu Gly 1970 1975 1980 Ser Glu Tyr Leu Asp Ile Ala Gly
Leu Lys Ile Pro Thr Glu Glu 1985 1990 1995 Met Lys Gly Asn Met Leu
Val Phe Ala Pro Thr Arg Asn Met Ala 2000 2005 2010 Val Glu Thr Ala
Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser Gly 2015 2020 2025 Tyr Tyr
Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val Thr 2030 2035 2040
Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu Ser 2045
2050 2055 Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr Gly
Leu 2060 2065 2070 Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met
Pro Phe Ile 2075 2080 2085 Val Thr Gly Leu Lys Arg Met Ala Val Thr
Ile Gly Glu Gln Ala 2090 2095 2100 Gln Arg Arg Gly Arg Val Gly Arg
Val Lys Pro Gly Arg Tyr Tyr 2105 2110 2115 Arg Ser Gln Glu Thr Ala
Ser Gly Ser Lys Asp Tyr His Tyr Asp 2120 2125 2130 Leu Leu Gln Ala
Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn Val 2135 2140 2145 Thr Lys
Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr Glu 2150 2155 2160
Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn Leu 2165
2170 2175 Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile Met
Ala 2180 2185 2190 Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr
Asn Ser Tyr 2195 2200 2205 Glu Asn Gln Ile Pro Val Leu Phe Pro Lys
Ile Lys Asn Gly Glu 2210 2215 2220 Val Thr Asp Ser Tyr Glu Asn Tyr
Thr Tyr Leu Asn Ala Arg Lys 2225 2230 2235 Leu Gly Glu Asp Val Pro
Ala Tyr Val Tyr Ala Thr Glu Asp Glu 2240 2245 2250 Asp Leu Ala Val
Asp Leu Leu Gly Met Asp Trp Pro Asp Pro Gly 2255 2260 2265 Asn Gln
Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val Thr 2270 2275 2280
Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe Gly 2285
2290 2295 Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro Met
Ile 2300 2305 2310 Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu
Asp Thr Thr 2315 2320 2325 His Leu Gln Phe Ala Pro Asn Ala Ile Arg
Thr Asp Gly Lys Asp 2330 2335 2340 Ser Glu Leu Lys Glu Leu Ala Val
Gly Asp Leu Asp Lys Tyr Val 2345 2350 2355 Asp Ala Leu Val Asp Tyr
Ser Lys Gln Gly Met Lys Phe Ile Lys 2360 2365 2370 Val Gln Ala Glu
Lys Val Arg Asp Ser Gln Ser Thr Lys Glu Gly 2375 2380 2385 Leu Gln
Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser Leu 2390 2395 2400
Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly Val 2405
2410 2415 His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly His
Glu 2420 2425 2430 Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala
Phe Gly Gly 2435 2440 2445 Glu Thr Val Ser Ala His Ile Lys Gln Val
Ala Val Asp Leu Val 2450 2455 2460 Val Tyr Tyr Ile Ile Asn Lys Pro
Ser Phe Pro Gly Asp Thr Glu 2465 2470 2475 Thr Gln Gln Glu Gly Arg
Arg Phe Val Ala Ser Leu Phe Ile Ser 2480 2485 2490 Ala Leu Ala Thr
Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn Leu 2495 2500 2505 Gln Arg
Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala Thr 2510 2515 2520
Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val Val 2525
2530 2535 Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile Arg
Lys 2540 2545 2550 Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser
Ala Ala Met 2555 2560 2565 Glu Ile Leu Asn Gln Asn Pro Ile Ser Val
Gly Ile Ser Val Met 2570 2575 2580 Leu Gly Val Gly Ala Ile Ala Ala
His Asn Ala Ile Glu Ser Ser 2585 2590 2595 Glu Gln Lys Arg Thr Leu
Leu Met Lys Val Phe Val Lys Asn Phe 2600 2605 2610 Leu Asp Gln Ala
Ala Thr Asp Glu Leu Val Lys Glu Asn Pro Glu 2615 2620 2625 Lys Ile
Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly Asn 2630 2635 2640
Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys Gly 2645
2650 2655 Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg Asn
Leu 2660 2665 2670 Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu
Gly Met Asp 2675 2680 2685 Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly
Asn Tyr Ile Leu Asp 2690 2695 2700 Leu Ile Phe Asn Leu His Asn Lys
Leu Asn Lys Gly Leu Lys Lys 2705 2710 2715 Leu Val Leu Gly Trp Ala
Pro Ala Pro Leu Ser Cys Asp Trp Thr 2720 2725 2730 Pro Ser Asp Glu
Arg Ile Ser Leu Pro His Asn Asn Tyr Leu Arg 2735 2740 2745 Val Glu
Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile Lys 2750 2755 2760
Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser Phe 2765
2770 2775 Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe Lys
Val 2780 2785 2790 Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys
Pro Val Ala 2795 2800 2805 Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr
Lys Gly Val Thr Ala 2810 2815 2820 Lys Leu Asp Tyr Asn Asn Gly Lys
Val Leu Leu Ala Thr Asn Lys 2825 2830 2835 Trp Glu Val Asp His Ala
Phe Leu Thr Arg Leu Val Lys Lys His 2840 2845 2850 Thr Gly Ile Gly
Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro Asp 2855 2860 2865 His Gln
Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys Asn 2870 2875 2880
Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr Tyr 2885
2890 2895 Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu Val
His 2900 2905 2910 Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val
Thr Val Thr 2915 2920 2925 Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu
Asp Leu Gly Thr Ile 2930
2935 2940 Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro Glu
Glu 2945 2950 2955 Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr
Glu Thr Ala 2960 2965 2970 Ile Thr Ile Thr Gly Glu Ala Glu Val Met
Thr Thr Gly Ile Thr 2975 2980 2985 Pro Val Val Glu Met Lys Glu Glu
Pro Gln Leu Asp His Gln Ser 2990 2995 3000 Thr Thr Leu Lys Val Gly
Leu Lys Glu Gly Glu Tyr Pro Gly Pro 3005 3010 3015 Gly Val Asn Pro
Asn His Leu Ala Glu Val Ile Asp Glu Lys Asp 3020 3025 3030 Asp Arg
Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser Thr Ser 3035 3040 3045
Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys Gly Asn 3050
3055 3060 Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg Ile
Leu 3065 3070 3075 Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys
Glu Leu Val 3080 3085 3090 Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu
Ala Leu Glu Ala Leu 3095 3100 3105 Ser Leu Gly Lys Pro Ile Lys Arg
Lys Thr Thr Thr Ala Met Ile 3110 3115 3120 Arg Arg Leu Ile Glu Pro
Glu Val Glu Glu Glu Leu Pro Asp Trp 3125 3130 3135 Phe Gln Ala Glu
Glu Pro Leu Phe Leu Glu Ala Lys Ile Gln Asn 3140 3145 3150 Asp Leu
Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys Ser Lys 3155 3160 3165
Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val Lys Glu 3170
3175 3180 Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp Ser
Thr 3185 3190 3195 Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu
Phe Glu Glu 3200 3205 3210 Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys
Ile Ser Lys Gly His 3215 3220 3225 Met Val Ser Ala Tyr Gln Leu Ala
Gln Gly Asn Trp Glu Pro Leu 3230 3235 3240 Gly Cys Gly Val Tyr Met
Gly Thr Ile Pro Ala Arg Arg Leu Lys 3245 3250 3255 Ile His Pro Tyr
Glu Ala Tyr Leu Lys Leu Lys Glu Leu Val Glu 3260 3265 3270 Val Glu
Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile Arg Glu 3275 3280 3285
His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly Asn Leu 3290
3295 3300 Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp Gln
Leu 3305 3310 3315 Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys
Ile Ile Gly 3320 3325 3330 Ser Thr Met Ala Ser Ala Gly Ile Arg Leu
Glu Lys Leu Pro Val 3335 3340 3345 Val Arg Ala Gln Thr Asp Thr Thr
Ser Phe His Gln Ala Ile Arg 3350 3355 3360 Glu Lys Ile Asp Lys Thr
Glu Asn Lys Gln Thr Pro Glu Leu His 3365 3370 3375 Glu Glu Leu Met
Lys Val Phe Asp Cys Leu Lys Ile Pro Glu Leu 3380 3385 3390 Lys Glu
Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu Ala Gly 3395 3400 3405
Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys Asn Ile 3410
3415 3420 Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln Leu
Ile 3425 3430 3435 Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr
Glu Thr Ala 3440 3445 3450 Ile Pro Lys Asn Glu Lys Arg Asp Val Ser
Asp Asp Trp Glu Ala 3455 3460 3465 Gly Glu Phe Val Asp Glu Lys Lys
Pro Arg Val Ile Gln Tyr Pro 3470 3475 3480 Asp Ala Lys Val Arg Leu
Ala Ile Thr Lys Val Met Tyr Lys Trp 3485 3490 3495 Val Lys Gln Lys
Pro Val Val Ile Pro Gly Tyr Glu Gly Lys Thr 3500 3505 3510 Pro Leu
Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp Asp Ser 3515 3520 3525
Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala Trp Asp 3530
3535 3540 Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp Ile
Gln 3545 3550 3555 Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu
Asp Thr Ile 3560 3565 3570 Thr Glu His Met Val Glu Val Pro Val Ile
Thr Ala Asp Gly Glu 3575 3580 3585 Val Tyr Ile Arg Asn Gly Gln Arg
Gly Ser Gly Gln Pro Asp Thr 3590 3595 3600 Ser Ala Gly Asn Ser Met
Leu Asn Val Leu Thr Met Ile Tyr Ala 3605 3610 3615 Phe Cys Lys Ser
Thr Gly Ile Pro Tyr Arg Gly Phe Ser Arg Val 3620 3625 3630 Ala Arg
Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile Thr Glu 3635 3640 3645
Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln Ile Leu 3650
3655 3660 His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp Lys
Met 3665 3670 3675 Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys
Ser His Thr 3680 3685 3690 Pro Val Pro Val Arg Trp Ala Asp Asn Thr
Ser Ser Tyr Met Ala 3695 3700 3705 Gly Arg Ser Thr Ala Thr Ile Leu
Ala Lys Met Ala Thr Arg Leu 3710 3715 3720 Asp Ser Ser Gly Glu Arg
Gly Ser Thr Ala Tyr Glu Lys Ala Val 3725 3730 3735 Ala Phe Ser Phe
Leu Leu Met Tyr Ser Trp Asn Pro Val Val Arg 3740 3745 3750 Arg Ile
Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile Ser Pro 3755 3760 3765
Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile Ala Ala 3770
3775 3780 Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys Arg
Thr 3785 3790 3795 Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met
Thr Thr Leu 3800 3805 3810 Gly Ile Trp Thr Lys His Thr Ser Lys Arg
Leu Ile Gln Ala Cys 3815 3820 3825 Val Glu Ile Gly Lys Arg Glu Gly
Thr Trp Leu Val Asn Ala Asp 3830 3835 3840 Arg Leu Ile Ala Gly Lys
Thr Gly Lys Phe Tyr Ile Pro Ser Thr 3845 3850 3855 Gly Val Thr Leu
Leu Gly Lys His Tyr Glu Glu Ile Asn Leu Lys 3860 3865 3870 Gln Lys
Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg Tyr Lys 3875 3880 3885
Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg Val Met 3890
3895 3900 Leu Met Thr Val Ala Ser Gly Ser Trp 3905 3910
83748PRTArtificial SequenceMutated BVDV XIKE-B-NdN 8Met Glu Leu Phe
Ser Asp Glu Gly Ser Lys Gly Ala Thr Ser Lys Lys 1 5 10 15 Gln Pro
Lys Pro Asp Arg Ile Glu Lys Gly Lys Met Lys Ile Ala Pro 20 25 30
Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro Asp Ala Thr Ile 35
40 45 Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys Gly Lys Val
Arg 50 55 60 Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys Asn
Lys Pro Pro 65 70 75 80 Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu
Ala Trp Ala Ile Leu 85 90 95 Ala Ala Val Leu Leu Gln Leu Val Thr
Gly Glu Asn Ile Thr Gln Trp 100 105 110 Asn Leu Met Asp Asn Gly Thr
Glu Gly Ile Gln Gln Ala Met Phe Leu 115 120 125 Arg Gly Val Asn Arg
Ser Leu His Gly Ile Trp Pro Glu Lys Ile Cys 130 135 140 Thr Gly Val
Pro Thr His Leu Ala Thr Asp Tyr Glu Leu Lys Glu Ile 145 150 155 160
Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr Thr Cys Cys Arg 165
170 175 Leu Gln Arg His Glu Trp Asn Lys Gly Trp Cys Asn Trp Phe His
Ile 180 185 190 Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln Asn Asn
Leu Thr Glu 195 200 205 Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys
Arg Tyr Asp Lys Glu 210 215 220 Thr Glu Leu Asn Ile Val Thr Gln Ala
Arg Asp Arg Pro Thr Thr Leu 225 230 235 240 Thr Gly Cys Lys Lys Gly
Lys Asn Phe Ser Phe Ala Gly Val Ile Leu 245 250 255 Asp Gly Pro Cys
Asn Phe Lys Val Ser Val Glu Asp Val Leu Phe Lys 260 265 270 Glu His
Asp Cys Gly Asn Met Leu Gln Glu Thr Ala Ile Gln Leu Leu 275 280 285
Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg Val Gly Thr Ala Lys 290
295 300 Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile Leu Gly Lys Lys
Leu 305 310 315 320 Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala
Ala Ser Pro Tyr 325 330 335 Cys Gly Val Glu Arg Lys Ile Gly Tyr Val
Trp Tyr Thr Lys Asn Cys 340 345 350 Thr Pro Ala Cys Leu Pro Arg Asn
Thr Arg Ile Ile Gly Pro Gly Lys 355 360 365 Phe Asp Thr Asn Ala Glu
Asp Gly Lys Ile Leu His Glu Met Gly Gly 370 375 380 His Leu Ser Glu
Phe Val Leu Leu Ser Leu Val Val Leu Ser Asp Phe 385 390 395 400 Ala
Pro Glu Thr Ala Ser Val Ile Tyr Leu Val Leu His Phe Ala Ile 405 410
415 Pro Gln Ser His Val Asp Val Asp Thr Cys Asp Lys Asn Gln Leu Asn
420 425 430 Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile Pro Gly Thr
Val Trp 435 440 445 Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp
Trp Pro Tyr Glu 450 455 460 Thr Thr Thr Val Phe Val Ile Glu Glu Ala
Gly Gln Val Ile Lys Leu 465 470 475 480 Met Leu Arg Ala Ile Arg Asp
Leu Thr Arg Ile Trp Asn Ala Ala Thr 485 490 495 Thr Thr Ala Phe Leu
Ile Phe Leu Val Lys Ala Leu Arg Gly Gln Leu 500 505 510 Ile Gln Gly
Leu Leu Trp Leu Met Leu Ile Thr Gly Ala Gln Gly Phe 515 520 525 Pro
Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile Ser Lys Asp Arg Lys 530 535
540 Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr Thr Trp His Leu Pro
545 550 555 560 Thr Lys Lys Ile Val Asp Ser Met Val His Val Trp Cys
Glu Gly Lys 565 570 575 Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu
Glu Arg Tyr Leu Val 580 585 590 Ala Val His Glu Arg Ala Leu Ser Thr
Ser Ala Glu Phe Met Gln Ile 595 600 605 Ser Asp Gly Thr Ile Gly Pro
Asp Val Ile Asp Met Pro Asp Asp Phe 610 615 620 Glu Phe Gly Leu Cys
Pro Cys Asp Ser Lys Pro Val Ile Lys Gly Lys 625 630 635 640 Phe Asn
Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln Met Val Cys Pro 645 650 655
Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala Asn Gln Asp Thr 660
665 670 Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg Thr Thr Pro Phe
Gln 675 680 685 Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly Glu
Asp Ile Tyr 690 695 700 Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile
Thr Gly Asp His Ser 705 710 715 720 Arg Leu Lys Asp Gly Pro Ile Lys
Lys Cys Lys Trp Cys Gly His Asp 725 730 735 Phe Val Asn Ser Glu Gly
Leu Pro His Tyr Pro Ile Gly Lys Cys Met 740 745 750 Leu Ile Asn Glu
Ser Gly Tyr Arg Tyr Val Asp Asp Thr Ser Cys Asp 755 760 765 Arg Gly
Gly Val Ala Ile Val Pro Ser Gly Thr Val Lys Cys Arg Ile 770 775 780
Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn Asp Leu Gly Pro 785
790 795 800 Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser Glu Gly Pro
Val Glu 805 810 815 Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr Leu
Pro Asn Lys Tyr 820 825 830 Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln
Tyr Met Leu Lys Gly Glu 835 840 845 Trp Gln Tyr Trp Phe Asp Leu Asp
Ser Val Asp His His Lys Asp Tyr 850 855 860 Phe Ser Glu Phe Ile Ile
Ile Ala Val Val Ala Leu Leu Gly Gly Lys 865 870 875 880 Tyr Val Leu
Trp Leu Leu Ile Thr Tyr Thr Ile Leu Ser Glu Gln Met 885 890 895 Ala
Met Gly Ala Gly Val Asn Thr Glu Glu Ile Val Met Ile Gly Asn 900 905
910 Leu Leu Thr Asp Ser Asp Ile Glu Val Val Val Tyr Phe Leu Leu Leu
915 920 925 Tyr Leu Ile Val Lys Glu Glu Leu Ala Arg Lys Trp Ile Ile
Leu Val 930 935 940 Tyr His Ile Leu Val Ala Asn Pro Met Lys Thr Ile
Gly Val Val Leu 945 950 955 960 Leu Met Leu Gly Gly Val Val Lys Ala
Ser Arg Ile Asn Ala Asp Asp 965 970 975 Gln Ser Ala Met Asp Pro Cys
Phe Leu Leu Val Thr Gly Val Val Ala 980 985 990 Val Leu Met Ile Ala
Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile Val 995 1000 1005 Ala Leu
Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser 1010 1015 1020
Ile Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala 1025
1030 1035 Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln
Leu 1040 1045 1050 Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg
Ser Leu Lys 1055 1060 1065 His Ile Asn Gln Met Glu Val Pro Glu Ile
Ser Met Pro Ser Trp 1070 1075 1080 Arg Pro Leu Ala Leu Val Leu Phe
Tyr Ile Thr Ser Thr Ala Ile 1085 1090 1095 Thr Thr Asn Trp Asp Ile
Asp Leu Ala Gly Phe Leu Leu Gln Trp 1100 1105 1110 Ala Pro Ala Val
Ile Met Met Ala Thr Met Trp Ala Asp Phe Leu 1115 1120 1125 Thr Leu
Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr 1130 1135 1140
Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly 1145
1150 1155 Lys Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys
Asp 1160 1165 1170 Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His
Lys Ser Gly 1175 1180 1185 Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu
Lys Ala Val Leu Ile 1190 1195 1200 Ser Cys Ile Ser Ser Gln Trp Gln
Val Val Tyr Ile Ser Tyr Leu 1205 1210 1215 Ile Leu Glu Ile Thr Tyr
Tyr Met His Arg Lys Ile Ile Asp Glu 1220 1225 1230 Val Ser Gly Gly
Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala Ile 1235 1240 1245 Ile Glu
Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu 1250 1255
1260
Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val 1265
1270 1275 Lys His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly
Glu 1280 1285 1290 Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile
Ile Lys Ala 1295 1300 1305 Ser Thr Leu Ser Lys Asn Arg His Cys Ile
Ile Cys Thr Ile Cys 1310 1315 1320 Glu Gly Lys Glu Trp Asn Gly Ala
Asn Cys Pro Lys Cys Gly Arg 1325 1330 1335 Gln Gly Lys Pro Ile Thr
Cys Gly Met Thr Leu Ala Asp Phe Glu 1340 1345 1350 Glu Lys His Tyr
Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser Cys 1355 1360 1365 Pro Val
Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg His 1370 1375 1380
Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr 1385
1390 1395 Ala Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala
Thr 1400 1405 1410 Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala
Glu Ile Gly 1415 1420 1425 Asp Leu Glu His Leu Gly Trp Val Leu Arg
Gly Pro Ala Val Cys 1430 1435 1440 Lys Lys Ile Thr Asn His Glu Lys
Cys His Val Asn Ile Met Asp 1445 1450 1455 Lys Leu Thr Ala Phe Phe
Gly Ile Met Pro Arg Gly Thr Thr Pro 1460 1465 1470 Arg Ala Pro Val
Arg Phe Pro Thr Ala Leu Leu Lys Val Arg Arg 1475 1480 1485 Gly Leu
Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile Ser 1490 1495 1500
Ser Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp 1505
1510 1515 Ser Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys
Met 1520 1525 1530 Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser
Gly Cys Pro 1535 1540 1545 Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro
Glu Ala Val Asn Ile 1550 1555 1560 Ser Gly Thr Lys Gly Ala Met Val
His Leu Gln Lys Thr Gly Gly 1565 1570 1575 Glu Phe Thr Cys Val Thr
Ala Ser Gly Thr Pro Ala Phe Phe Asp 1580 1585 1590 Leu Lys Asn Leu
Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu Ala 1595 1600 1605 Ser Ser
Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn Glu 1610 1615 1620
Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser 1625
1630 1635 Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr
Ser 1640 1645 1650 Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala
Thr Gly Ala 1655 1660 1665 Gly Lys Thr Thr Glu Leu Pro Arg Ser Val
Ile Glu Glu Ile Gly 1670 1675 1680 Arg His Lys Arg Val Leu Val Leu
Ile Pro Leu Arg Ala Ala Ala 1685 1690 1695 Glu Ser Val Tyr Gln Tyr
Met Arg Val Lys Tyr Pro Ser Ile Ser 1700 1705 1710 Phe Asn Leu Arg
Ile Gly Asp Met Lys Glu Gly Asp Met Ala Thr 1715 1720 1725 Gly Ile
Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln 1730 1735 1740
Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu 1745
1750 1755 Asp Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile
Gly 1760 1765 1770 Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val
Ala Met Thr 1775 1780 1785 Ala Thr Pro Ala Gly Thr Val Thr Thr Thr
Gly Gln Lys His Pro 1790 1795 1800 Ile Glu Glu Phe Ile Ala Pro Glu
Val Met Lys Gly Glu Asp Leu 1805 1810 1815 Gly Ser Glu Tyr Leu Asp
Ile Ala Gly Leu Lys Ile Pro Thr Glu 1820 1825 1830 Glu Met Lys Gly
Asn Met Leu Val Phe Ala Pro Thr Arg Asn Met 1835 1840 1845 Ala Val
Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser 1850 1855 1860
Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val 1865
1870 1875 Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile
Glu 1880 1885 1890 Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val
Asp Thr Gly 1895 1900 1905 Leu Lys Cys Glu Lys Arg Val Arg Ile Ser
Ser Lys Met Pro Phe 1910 1915 1920 Ile Val Thr Gly Leu Lys Arg Met
Ala Val Thr Ile Gly Glu Gln 1925 1930 1935 Ala Gln Arg Arg Gly Arg
Val Gly Arg Val Lys Pro Gly Arg Tyr 1940 1945 1950 Tyr Arg Ser Gln
Glu Thr Ala Ser Gly Ser Lys Asp Tyr His Tyr 1955 1960 1965 Asp Leu
Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn 1970 1975 1980
Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr 1985
1990 1995 Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn
Asn 2000 2005 2010 Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys
Asn Ile Met 2015 2020 2025 Ala Arg Thr Asp His Pro Glu Pro Ile Gln
Leu Ala Tyr Asn Ser 2030 2035 2040 Tyr Glu Asn Gln Ile Pro Val Leu
Phe Pro Lys Ile Lys Asn Gly 2045 2050 2055 Glu Val Thr Asp Ser Tyr
Glu Asn Tyr Thr Tyr Leu Asn Ala Arg 2060 2065 2070 Lys Leu Gly Glu
Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu Asp 2075 2080 2085 Glu Asp
Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp Pro 2090 2095 2100
Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val 2105
2110 2115 Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu
Phe 2120 2125 2130 Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His
Ile Pro Met 2135 2140 2145 Ile Thr Asp Ile Tyr Thr Leu Glu Asp His
Arg Leu Glu Asp Thr 2150 2155 2160 Thr His Leu Gln Phe Ala Pro Asn
Ala Ile Arg Thr Asp Gly Lys 2165 2170 2175 Asp Ser Glu Leu Lys Glu
Leu Ala Val Gly Asp Leu Asp Lys Tyr 2180 2185 2190 Val Asp Ala Leu
Val Asp Tyr Ser Lys Gln Gly Met Lys Phe Ile 2195 2200 2205 Lys Val
Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys Glu 2210 2215 2220
Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser 2225
2230 2235 Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp
Gly 2240 2245 2250 Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg
Leu Gly His 2255 2260 2265 Glu Thr Ala Phe Ala Thr Leu Val Val Lys
Trp Leu Ala Phe Gly 2270 2275 2280 Gly Glu Thr Val Ser Ala His Ile
Lys Gln Val Ala Val Asp Leu 2285 2290 2295 Val Val Tyr Tyr Ile Ile
Asn Lys Pro Ser Phe Pro Gly Asp Thr 2300 2305 2310 Glu Thr Gln Gln
Glu Gly Arg Arg Phe Val Ala Ser Leu Phe Ile 2315 2320 2325 Ser Ala
Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn 2330 2335 2340
Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala 2345
2350 2355 Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser
Val 2360 2365 2370 Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu
Ser Ile Arg 2375 2380 2385 Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr
Gly Ile Ser Ala Ala 2390 2395 2400 Met Glu Ile Leu Asn Gln Asn Pro
Ile Ser Val Gly Ile Ser Val 2405 2410 2415 Met Leu Gly Val Gly Ala
Ile Ala Ala His Asn Ala Ile Glu Ser 2420 2425 2430 Ser Glu Gln Lys
Arg Thr Leu Leu Met Lys Val Phe Val Lys Asn 2435 2440 2445 Phe Leu
Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn Pro 2450 2455 2460
Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly 2465
2470 2475 Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr
Lys 2480 2485 2490 Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala
Gly Arg Asn 2495 2500 2505 Leu Phe Thr Leu Ile Met Phe Glu Ala Phe
Glu Leu Leu Gly Met 2510 2515 2520 Asp Ser Glu Gly Lys Ile Arg Asn
Leu Ser Gly Asn Tyr Ile Leu 2525 2530 2535 Asp Leu Ile Phe Asn Leu
His Asn Lys Leu Asn Lys Gly Leu Lys 2540 2545 2550 Lys Leu Val Leu
Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp Trp 2555 2560 2565 Thr Pro
Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr Leu 2570 2575 2580
Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile 2585
2590 2595 Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly
Ser 2600 2605 2610 Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro
Asn Phe Lys 2615 2620 2625 Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile
Glu Val Lys Pro Val 2630 2635 2640 Ala Arg Leu Glu Gly Gln Val Asp
Leu Tyr Tyr Lys Gly Val Thr 2645 2650 2655 Ala Lys Leu Asp Tyr Asn
Asn Gly Lys Val Leu Leu Ala Thr Asn 2660 2665 2670 Lys Trp Glu Val
Asp His Ala Phe Leu Thr Arg Leu Val Lys Lys 2675 2680 2685 His Thr
Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro 2690 2695 2700
Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys 2705
2710 2715 Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe
Thr 2720 2725 2730 Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile
Glu Leu Val 2735 2740 2745 His Lys Asn Asn Leu Gln Glu Arg Glu Ile
Pro Thr Val Thr Val 2750 2755 2760 Thr Thr Trp Leu Ala Tyr Ser Phe
Val Asn Glu Asp Leu Gly Thr 2765 2770 2775 Ile Lys Pro Val Leu Gly
Glu Lys Val Ile Pro Glu Pro Pro Glu 2780 2785 2790 Glu Leu Ser Leu
Gln Pro Thr Val Arg Leu Val Thr Thr Glu Thr 2795 2800 2805 Ala Ile
Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly Ile 2810 2815 2820
Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln 2825
2830 2835 Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro
Gly 2840 2845 2850 Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile
Asp Glu Lys 2855 2860 2865 Asp Asp Arg Pro Phe Val Leu Ile Ile Gly
Asn Lys Gly Ser Thr 2870 2875 2880 Ser Asn Arg Ala Arg Thr Ala Lys
Asn Ile Arg Leu Tyr Lys Gly 2885 2890 2895 Asn Asn Pro Arg Glu Ile
Arg Asp Leu Met Ser Gln Gly Arg Ile 2900 2905 2910 Leu Thr Val Ala
Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu Leu 2915 2920 2925 Val Asp
Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu Ala 2930 2935 2940
Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met 2945
2950 2955 Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro
Asp 2960 2965 2970 Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala
Lys Ile Gln 2975 2980 2985 Asn Asp Leu Tyr His Leu Ile Gly Ser Val
Asp Ser Ile Lys Ser 2990 2995 3000 Lys Ala Lys Glu Leu Gly Ala Thr
Asp Asn Thr Lys Ile Val Lys 3005 3010 3015 Glu Val Gly Ala Arg Thr
Tyr Thr Met Lys Leu Ser Ser Trp Ser 3020 3025 3030 Thr Gln Val Thr
Lys Lys Gln Met Ser Leu Ala Pro Leu Phe Glu 3035 3040 3045 Glu Leu
Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys Gly 3050 3055 3060
His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro 3065
3070 3075 Leu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg
Leu 3080 3085 3090 Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys
Glu Leu Val 3095 3100 3105 Glu Val Glu Ser Ser Arg Ala Thr Ala Lys
Glu Ser Ile Ile Arg 3110 3115 3120 Glu His Asn Thr Trp Ile Leu Arg
Lys Val Arg His Glu Gly Asn 3125 3130 3135 Leu Arg Thr Lys Ser Met
Ile Asn Pro Gly Lys Ile Ser Asp Gln 3140 3145 3150 Leu Cys Arg Asp
Gly His Lys Arg Asn Ile Tyr Asn Lys Ile Ile 3155 3160 3165 Gly Ser
Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu Pro 3170 3175 3180
Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile 3185
3190 3195 Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu
Leu 3200 3205 3210 His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys
Ile Pro Glu 3215 3220 3225 Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp
Glu Gln Leu Glu Ala 3230 3235 3240 Gly Ile Asn Arg Lys Gly Ala Ala
Gly Tyr Leu Glu Ser Lys Asn 3245 3250 3255 Ile Gly Glu Val Leu Asp
Thr Glu Lys His Ile Val Glu Gln Leu 3260 3265 3270 Ile Lys Asp Leu
Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu Thr 3275 3280 3285 Ala Ile
Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp Glu 3290 3295 3300
Ala Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr 3305
3310 3315 Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr
Lys 3320 3325 3330 Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr
Glu Gly Lys 3335 3340 3345 Thr Pro Leu Phe Asp Ile Phe Asn Lys Val
Lys Lys Glu Trp Asp 3350 3355 3360 Ser Phe Gln Asp Pro Val Ala Val
Ser Phe Asp Thr Lys Ala Trp 3365 3370 3375 Asp Thr Gln Val Thr Ser
Arg Asp Leu Met Leu Ile Lys Asp Ile 3380 3385 3390 Gln Lys Tyr Tyr
Phe Lys Arg Ser Ile His Lys Phe Leu Asp Thr 3395 3400 3405 Ile Thr
Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp Gly 3410 3415 3420
Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp 3425
3430 3435 Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile
Tyr 3440 3445 3450 Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr
Arg Gly Phe Ser Arg 3455 3460 3465 Val Ala Arg Ile His Val Cys Gly
Asp Asp Gly Phe Leu Ile Thr 3470 3475 3480 Glu Arg Gly Leu Gly Leu
Lys Phe Ser Glu Lys Gly Met Gln Ile 3485 3490 3495 Leu His Glu Ala
Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp Lys 3500 3505 3510 Met Lys
Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser His 3515 3520 3525
Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr Met 3530
3535 3540 Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr
Arg 3545 3550 3555 Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr
Glu Lys Ala 3560 3565 3570 Val Ala Phe Ser Phe Leu Leu Met Tyr Ser
Trp Asn Pro Val Val 3575 3580 3585 Arg Arg Ile Cys Leu Leu Val Leu
Ser Gln Phe Pro Glu Ile Ser 3590 3595 3600 Pro Ser Lys Asn Thr Ile
Tyr Tyr Tyr Gln Gly Asp Pro Ile Ala 3605 3610 3615 Ala Tyr Arg Glu
Val Ile Gly Lys Gln Leu Cys Glu Leu Lys Arg 3620 3625 3630 Thr Gly
Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr Thr 3635 3640 3645
Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln Ala 3650
3655 3660 Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn
Ala 3665 3670 3675 Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr
Ile Pro Ser 3680 3685 3690 Thr Gly Val Thr Leu Leu Gly Lys His Tyr
Glu Glu Ile Asn Leu 3695 3700 3705 Lys Gln Lys Ala Ala Gln Pro Pro
Ile Glu Gly Val Asp Arg Tyr 3710 3715 3720 Lys Leu Gly Pro Ile Val
Asn Val Ile Leu Arg Arg Leu Arg Val 3725 3730 3735 Met Leu Met Thr
Val Ala Ser Gly Ser Trp 3740 3745 93913PRTArtificial SequenceXIKE-C
BVDV-Sequence 9Met Glu Leu Phe Ser Asn Glu Leu Leu Tyr Lys Thr Tyr
Lys Gln Lys 1 5 10 15 Pro Ala Gly Val Val Glu Pro Val Tyr Asp Val
Asn Gly Arg Pro Leu 20 25 30 Phe Gly Glu Ser Ser Asp Leu His Pro
Gln Ser Thr Leu Lys Leu Pro 35 40 45 His Gln Arg Gly Ser Ala Asn
Ile Leu Thr Asn Ala Arg Ser Leu Pro 50 55 60 Arg Lys Gly Asp Cys
Arg Arg Gly Asn Val Tyr Gly Pro Val Ser Gly 65 70 75 80 Ile Tyr Ile
Lys Pro Gly Pro Ile Tyr Tyr Gln Asp Tyr Val Gly Pro 85 90 95 Val
Tyr His Arg Ala Pro Leu Glu Leu Cys Arg Glu Ala Ser Met Cys 100 105
110 Glu Thr Thr Arg Arg Val Gly Arg Val Thr Gly Ser Asp Gly Lys Leu
115 120 125 Tyr His Ile Tyr Ile Cys Ile Asp Gly Cys Ile Leu Leu Lys
Arg Ala 130 135 140 Thr Arg Asn Gln Pro Glu Val Leu Lys Trp Val Tyr
Asn Arg Leu Asn 145 150 155 160 Cys Pro Leu Trp Val Thr Ser Cys Ser
Asp Glu Gly Ser Lys Gly Ala 165 170 175 Thr Ser Lys Lys Gln Pro Lys
Pro Asp Arg Ile Glu Lys Gly Lys Met 180 185 190 Lys Ile Ala Pro Lys
Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro 195 200 205 Asp Ala Thr
Ile Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys 210 215 220 Gly
Lys Val Arg Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys 225 230
235 240 Asn Lys Pro Pro Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu
Ala 245 250 255 Trp Ala Ile Leu Ala Ala Val Leu Leu Gln Leu Val Thr
Gly Glu Asn 260 265 270 Ile Thr Gln Trp Asn Leu Met Asp Asn Gly Thr
Glu Gly Ile Gln Gln 275 280 285 Ala Met Phe Leu Arg Gly Val Asn Arg
Ser Leu Leu Gly Ile Trp Pro 290 295 300 Glu Lys Ile Cys Thr Gly Val
Pro Thr His Leu Ala Thr Asp Tyr Glu 305 310 315 320 Leu Lys Glu Ile
Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr 325 330 335 Thr Cys
Cys Arg Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys 340 345 350
Asn Trp Phe His Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln 355
360 365 Asn Asn Leu Thr Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr
Cys 370 375 380 Arg Tyr Asp Lys Glu Thr Glu Leu Asn Ile Val Thr Gln
Ala Arg Asp 385 390 395 400 Arg Pro Thr Thr Leu Thr Gly Cys Lys Lys
Gly Lys Asn Phe Ser Phe 405 410 415 Ala Gly Val Ile Leu Asp Gly Pro
Cys Asn Phe Lys Val Ser Val Glu 420 425 430 Asp Val Leu Phe Lys Glu
His Asp Cys Gly Asn Met Leu Gln Glu Thr 435 440 445 Ala Ile Gln Leu
Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg 450 455 460 Val Gly
Thr Ala Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile 465 470 475
480 Leu Gly Lys Lys Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His
485 490 495 Ala Ala Ser Pro Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr
Val Trp 500 505 510 Tyr Thr Lys Asn Cys Thr Pro Ala Cys Leu Pro Arg
Asn Thr Arg Ile 515 520 525 Ile Gly Pro Gly Lys Phe Asp Thr Asn Ala
Glu Asp Gly Lys Ile Leu 530 535 540 His Glu Met Gly Gly His Leu Ser
Glu Phe Val Leu Leu Ser Leu Val 545 550 555 560 Val Leu Ser Asp Phe
Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val 565 570 575 Leu His Phe
Ala Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp 580 585 590 Lys
Asn Gln Leu Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile 595 600
605 Pro Gly Thr Val Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp
610 615 620 Trp Trp Pro Tyr Glu Thr Thr Thr Val Phe Val Ile Glu Glu
Ala Gly 625 630 635 640 Gln Val Ile Lys Leu Met Leu Arg Ala Ile Arg
Asp Leu Thr Arg Ile 645 650 655 Trp Asn Ala Ala Thr Thr Thr Ala Phe
Leu Ile Phe Leu Val Lys Ala 660 665 670 Leu Arg Gly Gln Leu Ile Gln
Gly Leu Leu Trp Leu Met Leu Ile Thr 675 680 685 Gly Ala Gln Gly Phe
Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile 690 695 700 Ser Lys Asp
Arg Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr 705 710 715 720
Thr Trp His Leu Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val 725
730 735 Trp Cys Glu Gly Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys
Glu 740 745 750 Glu Arg Tyr Leu Val Ala Val His Glu Arg Ala Leu Ser
Thr Ser Ala 755 760 765 Glu Phe Met Gln Ile Ser Asp Gly Thr Ile Gly
Pro Asp Val Ile Asp 770 775 780 Met Pro Asp Asp Phe Glu Phe Gly Leu
Cys Pro Cys Asp Ser Lys Pro 785 790 795 800 Val Ile Lys Gly Lys Phe
Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe 805 810 815 Gln Met Val Cys
Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu 820 825 830 Ala Asn
Gln Asp Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg 835 840 845
Thr Thr Pro Phe Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile 850
855 860 Gly Glu Asp Ile Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys
Ile 865 870 875 880 Thr Gly Asp His Ser Arg Leu Lys Asp Gly Pro Ile
Lys Lys Cys Lys 885 890 895 Trp Cys Gly His Asp Phe Val Asn Ser Glu
Gly Leu Pro His Tyr Pro 900 905 910 Ile Gly Lys Cys Met Leu Ile Asn
Glu Ser Gly Tyr Arg Tyr Val Asp 915 920 925 Asp Thr Ser Cys Asp Arg
Gly Gly Val Ala Ile Val Pro Ser Gly Thr 930 935 940 Val Lys Cys Arg
Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn 945 950 955 960 Asn
Asp Leu Gly Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser 965 970
975 Glu Gly Pro Val Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr
980 985 990 Leu Pro Asn Lys Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln
Gln Tyr 995 1000 1005 Met Leu Lys Gly Glu Trp Gln Tyr Trp Phe Asp
Leu Asp Ser Val 1010 1015 1020 Asp His His Lys Asp Tyr Phe Ser Glu
Phe Ile Ile Ile Ala Val 1025 1030 1035 Val Ala Leu Leu Gly Gly Lys
Tyr Val Leu Trp Leu Leu Ile Thr 1040 1045 1050 Tyr Thr Ile Leu Ser
Glu Gln Met Ala Met Gly Ala Gly Val Asn 1055 1060 1065 Thr Glu Glu
Ile Val Met Ile Gly Asn Leu Leu Thr Asp Ser Asp 1070 1075 1080 Ile
Glu Val Val Val Tyr Phe Leu Leu Leu Tyr Leu Ile Val Lys 1085 1090
1095 Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu Val Tyr His Ile Leu
1100 1105 1110 Val Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu Leu
Met Leu 1115 1120 1125 Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala
Asp Asp Gln Ser 1130 1135 1140 Ala Met Asp Pro Cys Phe Leu Leu Val
Thr Gly Val Val Ala Val 1145 1150 1155 Leu Met Ile Ala Arg Arg Glu
Pro Ala Thr Leu Pro Leu Ile Val 1160 1165 1170 Ala Leu Leu Ala Ile
Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser 1175 1180 1185 Ile Asp Val
Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala 1190 1195 1200 Ser
Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu 1205 1210
1215 Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys
1220 1225 1230 His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro
Ser Trp 1235 1240 1245 Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr
Ser Thr Ala Ile 1250 1255 1260 Thr Thr Asn Trp Asp Ile Asp Leu Ala
Gly Phe Leu Leu Gln Trp 1265 1270 1275 Ala Pro Ala Val Ile Met Met
Ala Thr Met Trp Ala Asp Phe Leu 1280 1285 1290 Thr Leu Ile Ile Val
Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr 1295 1300 1305 Phe Leu Lys
Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly 1310 1315 1320 Lys
Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp 1325 1330
1335 Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly
1340 1345 1350 Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val
Leu Ile 1355 1360 1365 Ser Cys Ile Ser Ser Gln Trp Gln Val Val Tyr
Ile Ser Tyr Leu 1370 1375 1380 Ile Leu Glu Ile Thr Tyr Tyr Met His
Arg Lys Ile Ile Asp Glu 1385 1390 1395 Val Ser Gly Gly Ala Asn Phe
Leu Ser Arg Leu Ile Ala Ala Ile 1400 1405 1410 Ile Glu Leu Asn Trp
Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu 1415 1420 1425 Lys Lys Leu
Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val 1430 1435 1440 Lys
His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu 1445 1450
1455 Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala
1460 1465 1470 Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr
Ile Cys 1475 1480 1485 Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro
Lys Cys Gly Arg 1490 1495 1500 Gln Gly Lys Pro Ile Thr Cys Gly Met
Thr Leu Ala Asp Phe Glu 1505 1510 1515 Glu Lys His Tyr Lys Lys Ile
Phe Ile Arg Glu Glu Ser Ser Cys 1520 1525 1530 Pro Val Pro Phe Asp
Pro Ser Cys His Cys Asn Tyr Phe Arg His 1535 1540 1545 Asp Gly Pro
Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr 1550 1555 1560 Ala
Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr 1565 1570
1575 Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly
1580 1585 1590 Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala
Val Cys 1595 1600 1605 Lys Lys Ile Thr Asn His Glu Lys Cys His Val
Asn Ile Met Asp 1610 1615 1620 Lys Leu Thr Ala Phe Phe Gly Ile Met
Pro Arg Gly Thr Thr Pro 1625 1630 1635 Arg Ala Pro Val Arg Phe Pro
Thr Ala Leu Leu Lys Val Arg Arg 1640 1645 1650 Gly Leu Glu Thr Gly
Trp Ala Tyr Thr His Gln Gly Gly Ile Ser 1655 1660 1665 Ser Val Asp
His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp 1670 1675 1680 Ser
Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met 1685 1690
1695 Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro
1700 1705 1710 Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val
Asn Ile 1715 1720 1725 Ser Gly Thr Lys Gly Ala Met Val His Leu Gln
Lys Thr Gly Gly 1730 1735 1740 Glu Phe Thr Cys Val Thr Ala Ser Gly
Thr Pro Ala Phe Phe Asp 1745 1750 1755 Leu Lys Asn Leu Lys Gly Trp
Ser Gly Leu Pro Ile Phe Glu Ala 1760 1765 1770 Ser Ser Gly Arg Val
Val Gly Arg Val Lys Val Gly Lys Asn Glu 1775 1780 1785 Asp Ser Lys
Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser 1790 1795 1800 Lys
Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser 1805 1810
1815 Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly Ala
1820 1825 1830 Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu
Ile Gly 1835 1840 1845 Arg His Lys Arg Val Leu Val Leu Ile Pro Leu
Arg Ala Ala Ala 1850 1855 1860 Glu Ser Val Tyr Gln Tyr Met Arg Val
Lys Tyr Pro Ser Ile Ser 1865 1870 1875 Phe Asn Leu Arg Ile Gly Asp
Met Lys Glu Gly Asp Met Ala Thr 1880 1885 1890 Gly Ile Thr Tyr Ala
Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln 1895 1900 1905 Pro Lys Leu
Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu 1910 1915 1920 Asp
Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly 1925 1930
1935 Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met Thr
1940
1945 1950 Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His
Pro 1955 1960 1965 Ile Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly
Glu Asp Leu 1970 1975 1980 Gly Ser Glu Tyr Leu Asp Ile Ala Gly Leu
Lys Ile Pro Thr Glu 1985 1990 1995 Glu Met Lys Gly Asn Met Leu Val
Phe Ala Pro Thr Arg Asn Met 2000 2005 2010 Ala Val Glu Thr Ala Lys
Lys Leu Lys Ala Lys Gly Tyr Asn Ser 2015 2020 2025 Gly Tyr Tyr Tyr
Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val 2030 2035 2040 Thr Ser
Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu 2045 2050 2055
Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr Gly 2060
2065 2070 Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro
Phe 2075 2080 2085 Ile Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile
Gly Glu Gln 2090 2095 2100 Ala Gln Arg Arg Gly Arg Val Gly Arg Val
Lys Pro Gly Arg Tyr 2105 2110 2115 Tyr Arg Ser Gln Glu Thr Ala Ser
Gly Ser Lys Asp Tyr His Tyr 2120 2125 2130 Asp Leu Leu Gln Ala Gln
Arg Tyr Gly Ile Glu Asp Gly Ile Asn 2135 2140 2145 Val Thr Lys Ser
Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr 2150 2155 2160 Glu Glu
Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn 2165 2170 2175
Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile Met 2180
2185 2190 Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn
Ser 2195 2200 2205 Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile
Lys Asn Gly 2210 2215 2220 Glu Val Thr Asp Ser Tyr Glu Asn Tyr Thr
Tyr Leu Asn Ala Arg 2225 2230 2235 Lys Leu Gly Glu Asp Val Pro Ala
Tyr Val Tyr Ala Thr Glu Asp 2240 2245 2250 Glu Asp Leu Ala Val Asp
Leu Leu Gly Met Asp Trp Pro Asp Pro 2255 2260 2265 Gly Asn Gln Gln
Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val 2270 2275 2280 Thr Gly
Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe 2285 2290 2295
Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro Met 2300
2305 2310 Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp
Thr 2315 2320 2325 Thr His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr
Asp Gly Lys 2330 2335 2340 Asp Ser Glu Leu Lys Glu Leu Ala Val Gly
Asp Leu Asp Lys Tyr 2345 2350 2355 Val Asp Ala Leu Val Asp Tyr Ser
Lys Gln Gly Met Lys Phe Ile 2360 2365 2370 Lys Val Gln Ala Glu Lys
Val Arg Asp Ser Gln Ser Thr Lys Glu 2375 2380 2385 Gly Leu Gln Thr
Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser 2390 2395 2400 Leu Thr
Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly 2405 2410 2415
Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly His 2420
2425 2430 Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe
Gly 2435 2440 2445 Gly Glu Thr Val Ser Ala His Ile Lys Gln Val Ala
Val Asp Leu 2450 2455 2460 Val Val Tyr Tyr Ile Ile Asn Lys Pro Ser
Phe Pro Gly Asp Thr 2465 2470 2475 Glu Thr Gln Gln Glu Gly Arg Arg
Phe Val Ala Ser Leu Phe Ile 2480 2485 2490 Ser Ala Leu Ala Thr Tyr
Thr Tyr Lys Thr Trp Asn Tyr Asn Asn 2495 2500 2505 Leu Gln Arg Val
Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala 2510 2515 2520 Thr Ser
Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val 2525 2530 2535
Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile Arg 2540
2545 2550 Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala
Ala 2555 2560 2565 Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly
Ile Ser Val 2570 2575 2580 Met Leu Gly Val Gly Ala Ile Ala Ala His
Asn Ala Ile Glu Ser 2585 2590 2595 Ser Glu Gln Lys Arg Thr Leu Leu
Met Lys Val Phe Val Lys Asn 2600 2605 2610 Phe Leu Asp Gln Ala Ala
Thr Asp Glu Leu Val Lys Glu Asn Pro 2615 2620 2625 Glu Lys Ile Ile
Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly 2630 2635 2640 Asn Pro
Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys 2645 2650 2655
Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg Asn 2660
2665 2670 Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly
Met 2675 2680 2685 Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn
Tyr Ile Leu 2690 2695 2700 Asp Leu Ile Phe Asn Leu His Asn Lys Leu
Asn Lys Gly Leu Lys 2705 2710 2715 Lys Leu Val Leu Gly Trp Ala Pro
Ala Pro Leu Ser Cys Asp Trp 2720 2725 2730 Thr Pro Ser Asp Glu Arg
Ile Ser Leu Pro His Asn Asn Tyr Leu 2735 2740 2745 Arg Val Glu Thr
Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile 2750 2755 2760 Lys Asn
Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser 2765 2770 2775
Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe Lys 2780
2785 2790 Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro
Val 2795 2800 2805 Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys
Gly Val Thr 2810 2815 2820 Ala Lys Leu Asp Tyr Asn Asn Gly Lys Val
Leu Leu Ala Thr Asn 2825 2830 2835 Lys Trp Glu Val Asp His Ala Phe
Leu Thr Arg Leu Val Lys Lys 2840 2845 2850 His Thr Gly Ile Gly Phe
Lys Gly Ala Tyr Leu Gly Asp Arg Pro 2855 2860 2865 Asp His Gln Asp
Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys 2870 2875 2880 Asn Ser
Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr 2885 2890 2895
Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu Val 2900
2905 2910 His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr
Val 2915 2920 2925 Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp
Leu Gly Thr 2930 2935 2940 Ile Lys Pro Val Leu Gly Glu Lys Val Ile
Pro Glu Pro Pro Glu 2945 2950 2955 Glu Leu Ser Leu Gln Pro Thr Val
Arg Leu Val Thr Thr Glu Thr 2960 2965 2970 Ala Ile Thr Ile Thr Gly
Glu Ala Glu Val Met Thr Thr Gly Ile 2975 2980 2985 Thr Pro Val Val
Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln 2990 2995 3000 Ser Thr
Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro Gly 3005 3010 3015
Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu Lys 3020
3025 3030 Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser
Thr 3035 3040 3045 Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu
Tyr Lys Gly 3050 3055 3060 Asn Asn Pro Arg Glu Ile Arg Asp Leu Met
Ser Gln Gly Arg Ile 3065 3070 3075 Leu Thr Val Ala Leu Lys Glu Leu
Asp Pro Glu Leu Lys Glu Leu 3080 3085 3090 Val Asp Tyr Lys Gly Thr
Phe Leu Asn Arg Glu Ala Leu Glu Ala 3095 3100 3105 Leu Ser Leu Gly
Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met 3110 3115 3120 Ile Arg
Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro Asp 3125 3130 3135
Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile Gln 3140
3145 3150 Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys
Ser 3155 3160 3165 Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys
Ile Val Lys 3170 3175 3180 Glu Val Gly Ala Arg Thr Tyr Thr Met Lys
Leu Ser Ser Trp Ser 3185 3190 3195 Thr Gln Val Thr Lys Lys Gln Met
Ser Leu Ala Pro Leu Phe Glu 3200 3205 3210 Glu Leu Leu Leu Lys Cys
Pro Pro Cys Ser Lys Ile Ser Lys Gly 3215 3220 3225 His Met Val Ser
Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro 3230 3235 3240 Leu Gly
Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg Leu 3245 3250 3255
Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu Val 3260
3265 3270 Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile
Arg 3275 3280 3285 Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg His
Glu Gly Asn 3290 3295 3300 Leu Arg Thr Lys Ser Met Ile Asn Pro Gly
Lys Ile Ser Asp Gln 3305 3310 3315 Leu Cys Arg Asp Gly His Lys Arg
Asn Ile Tyr Asn Lys Ile Ile 3320 3325 3330 Gly Ser Thr Met Ala Ser
Ala Gly Ile Arg Leu Glu Lys Leu Pro 3335 3340 3345 Val Val Arg Ala
Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile 3350 3355 3360 Arg Glu
Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu Leu 3365 3370 3375
His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro Glu 3380
3385 3390 Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu
Ala 3395 3400 3405 Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu
Ser Lys Asn 3410 3415 3420 Ile Gly Glu Val Leu Asp Thr Glu Lys His
Ile Val Glu Gln Leu 3425 3430 3435 Ile Lys Asp Leu Arg Lys Gly Lys
Lys Ile Arg Tyr Tyr Glu Thr 3440 3445 3450 Ala Ile Pro Lys Asn Glu
Lys Arg Asp Val Ser Asp Asp Trp Glu 3455 3460 3465 Ala Gly Glu Phe
Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr 3470 3475 3480 Pro Asp
Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr Lys 3485 3490 3495
Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly Lys 3500
3505 3510 Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp
Asp 3515 3520 3525 Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr
Lys Ala Trp 3530 3535 3540 Asp Thr Gln Val Thr Ser Arg Asp Leu Met
Leu Ile Lys Asp Ile 3545 3550 3555 Gln Lys Tyr Tyr Phe Lys Arg Ser
Ile His Lys Phe Leu Asp Thr 3560 3565 3570 Ile Thr Glu His Met Val
Glu Val Pro Val Ile Thr Ala Asp Gly 3575 3580 3585 Glu Val Tyr Ile
Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp 3590 3595 3600 Thr Ser
Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile Tyr 3605 3610 3615
Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser Arg 3620
3625 3630 Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile
Thr 3635 3640 3645 Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly
Met Gln Ile 3650 3655 3660 Leu His Glu Ala Gly Lys Pro Gln Lys Ile
Thr Glu Gly Asp Lys 3665 3670 3675 Met Lys Val Ala Tyr Arg Phe Glu
Asp Ile Glu Phe Cys Ser His 3680 3685 3690 Thr Pro Val Pro Val Arg
Trp Ala Asp Asn Thr Ser Ser Tyr Met 3695 3700 3705 Ala Gly Arg Ser
Thr Ala Thr Ile Leu Ala Lys Met Ala Thr Arg 3710 3715 3720 Leu Asp
Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys Ala 3725 3730 3735
Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val Val 3740
3745 3750 Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile
Ser 3755 3760 3765 Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp
Pro Ile Ala 3770 3775 3780 Ala Tyr Arg Glu Val Ile Gly Lys Gln Leu
Cys Glu Leu Lys Arg 3785 3790 3795 Thr Gly Phe Glu Lys Leu Ala Gly
Leu Asn Leu Ser Met Thr Thr 3800 3805 3810 Leu Gly Ile Trp Thr Lys
His Thr Ser Lys Arg Leu Ile Gln Ala 3815 3820 3825 Cys Val Glu Ile
Gly Lys Arg Glu Gly Thr Trp Leu Val Asn Ala 3830 3835 3840 Asp Arg
Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro Ser 3845 3850 3855
Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn Leu 3860
3865 3870 Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg
Tyr 3875 3880 3885 Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg
Leu Arg Val 3890 3895 3900 Met Leu Met Thr Val Ala Ser Gly Ser Trp
3905 3910 1012332DNAArtificial SequenceXIKE-C BVDV-Sequence
10gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg
60caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag
120gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc
gaacccctga 180gtacagggga gtcgtcaatg gttcgacact ccattagtcg
aggagtctcg agatgccatg 240tggacgaggg catgcccacg gcacatctta
acccatgcgg gggttgcatg ggtgaaagcg 300ctattcgtgg cgttatggac
acagcctgat agggtgtagc agagacctgc tattccgcta 360gtaaaaactc
tgctgtacat ggcacatgga gttgttttca aatgaacttt tatacaaaac
420atataaacaa aaaccagcag gcgtcgtgga acctgtttac gacgtcaacg
ggcgcccact 480gtttggagag agcagtgact tgcacccgca gtcaacacta
aaactaccac accaacgagg 540cagcgccaac atcctgacca atgctaggtc
cctaccgcgg aaaggtgact gccggagagg 600taatgtgtat ggaccggtga
gtggcatcta tatcaaacca ggaccgatct actaccagga 660ttatgtgggc
cccgtctatc atagagcccc actggaacta tgtagggagg caagtatgtg
720cgaaacaact aggagagttg gcagagtgac cggtagtgat gggaaattat
atcatatcta 780catctgcata gatgggtgta tcctcctgaa gagggcgact
aggaaccaac cagaagtcct 840gaaatgggta tacaacagat taaattgtcc
tttatgggtc accagctgct ccgatgaagg 900gagcaagggt gctacaagta
agaagcagcc taagccagat aggatagaaa aaggtaagat 960gaaaatagcc
ccaaaagaga cagaaaaaga ttgcaaaacc agaccccccg acgcgactat
1020agtagtagaa ggggttaagt accaggtgaa gaaaaaagga aaggtaaggg
gaaaaaatac 1080tcaagatggg ttatatcaca acaagaataa gccccctgaa
tcaagaaaaa aattggaaaa 1140ggcactgctg gcttgggcca tcttagcagc
ggtcctgctt cagctggtaa caggagagaa 1200tatcacccag tggaacttga
tggacaacgg caccgaggga atacagcaag cgatgttcct 1260aagaggggtg
aacaggagtc tattaggaat ttggccagag aaaatttgca ccggagtacc
1320aactcactta gcaacagact atgagcttaa agagatagtg gggatgatgg
acgcgagtga 1380gaagaccaac tacacgtgtt gcaggttgca aagacatgag
tggaataaac atggttggtg 1440taactggttt catatagaac cgtggatatg
gttgatgaac aaaacccaaa acaacctgac 1500agaagggcaa ccgcttaggg
agtgtgctgt gacttgtagg tatgacaagg aaacagaatt 1560gaacatcgtg
acacaggcta gggacagacc tacaactctg acaggttgca agaaaggcaa
1620gaatttctct ttcgcaggtg ttatactgga tgggccctgt aactttaaag
tatcggttga 1680agatgtgctg ttcaaggagc acgattgcgg caacatgctg
caagagaccg cgatacagct 1740actcgatggg gcaaccaaca ccattgaggg
agcaagggta gggacggcca agttgacaac 1800ctggttaggg aagcaattag
ggatccttgg taagaagttg gagaacaaaa gcaaagcatg 1860gtttggtgca
catgcagcaa gtccatactg cggagtggag aggaagatcg gttacgtatg
1920gtatacaaaa aactgcactc cagcttgcct tccaagaaac actagaataa
taggccccgg 1980gaaatttgat accaacgccg aagatggaaa aatactccat
gagatggggg ggcacctctc 2040agaatttgtc ctattgtcct tggtggttct
gtctgacttt gccccggaaa ccgcgagcgt 2100catctacttg gttctacatt
ttgcgatccc gcaaagccac gttgatgtag acacatgcga 2160caagaaccag
ctgaatttaa cggtagcaac cacagtagca gaggtcatac cagggacagt
2220gtggaaccta gggaagtatg tctgcataag accagactgg tggccatatg
agacgacgac 2280agtcttcgtc atagaggaag cagggcaagt aatcaaattg
atgctaaggg ccatcagaga 2340cttaactagg atatggaatg ctgccactac
cacagctttc ttaatctttt tagtaaaagc 2400actgagggga caactaatcc
aagggctatt gtggctgatg ctaataacag gagcacaggg 2460cttccctgaa
tgcaaagagg gcttccaata tgccatatct aaagacagga aaatggggtt
2520attggggcca gagagcttaa ctacaacatg gcacctcccc accaaaaaaa
tagtggattc 2580catggtgcat gtatggtgtg aaggaaaaga cttgaaaata
ttaaaaatgt gcacaaagga 2640agagaggtat ctagtggctg tgcacgagag
agccttatca accagtgccg agtttatgca 2700gatcagtgat gggacaatag
gcccagacgt gatagatatg cctgatgact ttgagtttgg 2760actctgccct
tgtgactcaa aaccagtgat aaagggcaaa tttaatgcca gcttactgaa
2820tggaccagct ttccagatgg tatgcccaca ggggtggact ggtacaatag
aatgcaccct 2880agcgaaccaa gacaccttgg acacaactgt cattaggaca
tatagaagaa ctaccccatt 2940tcagcggaga aaatggtgta cctatgaaaa
aataataggg gaagatatct atgaatgcat 3000tctaggtgga aactggacat
gcataaccgg tgaccatagc aggttgaaag acggacctat 3060caagaagtgt
aagtggtgtg gccatgactt cgtcaactca gaggggctac cacactaccc
3120aataggcaag tgcatgctca tcaacgagag tgggtacagg tatgtagatg
acacctcttg 3180cgataggggt ggtgtagcca tagttccatc tggcaccgta
aagtgtagaa taggtaacgt 3240cacggtgcaa gttatcgcta ctaacaatga
tctgggaccc atgccttgca gcccagctga 3300agtgatagca agtgaaggac
cagtggaaaa gactgcatgc acattcaact attcaaggac 3360tctacctaat
aagtattatg agccaaggga ccggtacttc caacaataca tgttaaaagg
3420ggagtggcaa tattggttcg acctggattc tgtagaccac cacaaagact
acttctcaga 3480gttcataatc atagcagtgg tcgccttgtt gggtggtaag
tacgtactgt ggctcttgat 3540aacatacaca atactgtctg agcagatggc
tatgggtgct ggagtgaata ctgaagagat 3600agtcatgata ggcaatttgc
tgacagacag tgatattgag gttgtggttt atttccttct 3660tctgtactta
atagttaaag aggaactggc gaggaaatgg attatactgg tataccacat
3720ccttgtagcc aaccctatga aaacaattgg ggtcgtctta ctaatgctag
ggggagtggt 3780gaaggccagc agaatcaatg ctgatgacca aagtgctatg
gacccatgct ttcttctcgt 3840gacaggcgta gtggctgttt tgatgatcgc
tagaagagaa cctgccacat taccactgat 3900tgtagcattg ctagcaataa
gaacatcagg attcctactg cccgctagca ttgatgtaac 3960tgtagcagta
gtattaattg tacttttgtt ggctagctac ataacagact actttagata
4020taaaaagtgg cttcaactct tatttagtct gatagctggt atctttatta
taaggagctt 4080aaaacatatc aaccagatgg aggtaccaga aatatctatg
ccaagttgga gacctctagc 4140tctggtcctt ttctatataa catctacagc
aataaccact aattgggaca ttgacttagc 4200aggcttcctg ctgcaatggg
cgccagcagt gatcatgatg gctaccatgt gggcagactt 4260tttgactctg
atcatagtcc tgcccagtta cgagttatct aagctttact tcctaaagaa
4320cgtcaggaca gacgtggaaa agaactggct cggcaaagtg aaatacagac
agatcagttc 4380agtttatgac atctgtgaca gtgaggaagc agtgtaccta
tttccatcaa ggcataagag 4440tggaagcagg ccagatttca tattaccttt
tttgaaagcc gtgttaataa gctgcatcag 4500cagccaatgg caagtggttt
acatttctta cctaatactg gaaattacat actatatgca 4560caggaaaatc
atagatgagg tgtcaggagg agcaaatttt ctatcaagac tcatagcagc
4620catcatagaa ttaaattggg ccatagatga tgaggaatgt aaaggactga
agaaactgta 4680tctcttgtca gggagagcga agaatttgat agttaaacat
aaggtaagaa atgaagccgt 4740ccacagatgg tttggtgagg aggaaatata
cggggcaccc aaggtgatca ctatcataaa 4800agctagtacc ctaagtaaaa
acaggcactg cataatctgc acgatctgtg aagggaaaga 4860atggaatgga
gccaactgcc caaagtgtgg aagacaagga aagcccataa catgtggaat
4920gacactcgca gactttgagg agaaacatta caaaaagata tttataagag
aagaatcttc 4980ttgtcctgtg ccttttgatc cttcttgcca ttgtaattat
tttcgccacg atgggccttt 5040caggaaagag tataagggtt acgtccaata
cacagccaga ggacaactct ttctgaggaa 5100cctaccaatt ctagcgacga
agatgaagct attaatggtg ggaaacctcg gcgcagaaat 5160tggcgacctg
gaacatctag gatgggtact gagagggcca gccgtgtgca aaaaaattac
5220caaccatgag aagtgccacg taaacatcat ggataagcta actgcatttt
ttggaatcat 5280gcctagaggc acgaccccta gggcacctgt gaggttcccc
acagcactac taaaagtgag 5340aagggggcta gagacgggat gggcttacac
gcaccaagga gggatcagct cggtagacca 5400tgtcacagcc ggaaaggatt
tactagtgtg tgacagtatg ggcaggacca gggttgtctg 5460tcatagtaac
aataagatga ctgatgagac tgagtatggc atcaagaccg actcagggtg
5520tcccgaaggt gcgaggtgtt acgtgctaaa cccagaagct gttaacattt
ctggcacaaa 5580aggagctatg gtacacctcc agaaaacggg gggggagttc
acatgtgtca ctgcctcagg 5640gaccccggct ttcttcgatc tgaaaaatct
aaaaggctgg tccgggctac caatttttga 5700agcatccagt ggcagggtgg
ttggtagggt gaaagtcggc aagaatgagg attccaagcc 5760caccaaacta
atgagcggaa tccagacagt gtctaagaac cagacagacc tagcggacat
5820cgtaaaaaaa ttgactagta tgaacagagg agagttcaaa cagataacat
tagccactgg 5880ggcaggaaaa actacggaac tgccaaggtc cgtcatagag
gagataggga ggcacaaaag 5940ggtcttagtc ctgataccat tgagagcagc
agcagagtca gtgtatcagt atatgagagt 6000gaagtaccca agtatatctt
tcaatttgag aataggagat atgaaggaag gtgacatggc 6060cactggtatc
acctacgcct catatgggta cttttgtcag cttcctcagc ccaaactgag
6120agctgccatg gtagagtact catatatatt cttagatgag taccactgtg
ctacacccga 6180gcaattagca ataattggaa agatacacag gtttgctgaa
aatcttagag tggtagcaat 6240gacagcaacc ccagctggaa cggtcacaac
gactggtcag aaacacccta tagaggagtt 6300catagcccca gaggtgatga
aaggtgaaga tctaggtagt gaatacttgg atattgcagg 6360gttgaagata
ccgactgaag agatgaaagg caacatgctc gtgttcgcgc caactaggaa
6420catggcagta gaaacagcta agaaattgaa ggctaaggga tacaactctg
gatactatta 6480cagtggggaa aacccagaga acttgagggt ggtaacctcg
caatccccgt atgtggtagt 6540agccaccaat gccatagagt caggtgtgac
attaccagac ttagacacag ttgtagacac 6600tggactaaag tgtgagaaga
gggtgaggat ttcttcaaaa atgcccttca ttgtaacagg 6660acttaagaga
atggcagtca caatcggaga gcaagcccag cgcaggggta gagtaggaag
6720agtcaagcca ggtaggtact ataggagtca agaaacagct tcagggtcaa
aagattacca 6780ttacgaccta ctgcaagccc agaggtacgg aatagaagat
ggaattaatg taacaaagtc 6840attcagggag atgaactatg attggagcct
ttacgaagag gacagcttga tgataactca 6900actcgaggtc cttaacaacc
tccttatatc agaagacctg cctgccgcag tgaagaacat 6960catggcccgg
accgatcacc cagaacccat acaactggcc tataacagtt atgaaaacca
7020aattccagtg ctgttcccaa agatcaaaaa tggtgaggtg acagacagtt
atgagaatta 7080cacatatctc aatgcaagaa aattaggaga ggacgtgccg
gcatatgtgt acgccacaga 7140ggatgaggat ctagcagtgg atcttctggg
tatggattgg ccggacccag gcaaccaaca 7200ggtggtagag acagggaggg
cattaaaaca agtaactggc ttatccacag cagaaaacgc 7260cctcttgata
gccctattcg gctacgtcgg gtaccagaca ctttcaaaaa ggcacatacc
7320catgattact gacatctata cacttgaaga ccacaggctt gaggacacaa
cccacctcca 7380gtttgcccca aacgctataa ggaccgacgg caaggactca
gagttgaagg aattagctgt 7440gggagacctt gataaatatg tggacgcact
ggtagactac tccaaacaag ggatgaaatt 7500catcaaagtc caagctgaaa
aggtcagaga ctcccagtct acgaaggaag gcttgcaaac 7560cattaaggag
tatgtggata agtttataca atcactaaca gagaataagg aggagatcat
7620caggtatgga ctatggggag ttcacacggc actctacaaa agcttggcag
cgagactggg 7680gcatgaaaca gcttttgcaa ctttagtggt aaaatggttg
gcttttgggg gcgaaacggt 7740atctgctcac atcaagcaag tagcagttga
tctagtagta tattatatca tcaacaaacc 7800atcttttcct ggagatacag
agacccaaca agaggggagg aagtttgtgg ctagtctttt 7860tatatctgca
ctagcaacat acacatataa aacctggaat tacaacaatc tgcaacgggt
7920tgtcgaacct gccttagctt acctcccata tgctacaagt gccttgaagt
tgttcacacc 7980cacaagatta gagagtgtgg tcatactcag ttctacaatt
tacaagacat acctctctat 8040aaggaagggt aagagtgacg gcttgttagg
tacaggcata agtgcagcca tggagatctt 8100aaaccaaaac ccaatctcag
taggtatatc tgtgatgctg ggggtaggtg ccatcgccgc 8160ccataatgca
atagaatcta gtgaacagaa aagaactttg ctgatgaagg tctttgtaaa
8220aaacttctta gaccaagcag caacagatga gctagtcaaa gagaaccctg
aaaaaataat 8280catggctcta tttgaagcag tccagaccat aggaaacccc
ctaagactca tctaccatct 8340gtacggggtg tactataagg ggtgggaagc
aaaagaactc gcagagaaaa ctgctggccg 8400caacttattc acattgatca
tgtttgaggc ctttgagctt ttaggtatgg actcagaagg 8460aaagataaga
aacttgtcag gcaactacat actggactta atcttcaact tgcataataa
8520attaaacaag gggctcaaaa aactagtcct tgggtgggct cctgcacctt
tgagctgtga 8580ttggacacca agtgatgaga gaataagcct acctcataac
aactacttaa gggtagaaac 8640caggtgtcct tgtggctatg agatgaaggc
aataaaaaat gttgctggta aattgacaaa 8700agttgaagaa aaggggtcct
tcctatgcag gaatagatta gggagaggac ctccaaactt 8760caaagtaaca
aagttctatg atgataactt gatagaagtc aagccagtag ctaggctaga
8820aggccaggtg gacctctatt acaagggagt aacagctaag ttagactaca
acaatgggaa 8880agtactgtta gctaccaaca agtgggaggt ggaccacgct
ttcctgacca gactagtaaa 8940gaagcacaca gggataggtt ttaaaggtgc
atatttgggt gaccgaccag accatcaaga 9000tcttgtcgat agagattgtg
caactataac gaagaactca gtacagttcc taaaaatgaa 9060gaagggttgc
gctttcacat atgacctaac aatctctaac cttgtcaggc ttattgaact
9120agtccataag aataatttac aagaaagaga gatccctacc gtgacagtaa
ctacttggct 9180tgcatattct tttgtcaatg aagacctggg gactatcaag
cctgtattgg gggagaaagt 9240catcccagaa ccccccgagg agttgagtct
ccaacccacc gtgagactag tcaccactga 9300aacagcaata accataacag
gggaggctga agtgatgacg acagggatca caccagtggt 9360agagatgaaa
gaagaacctc agctggacca ccagtcaact accctaaagg tagggttgaa
9420ggaaggggaa tatccagggc caggagttaa ccctaaccat ttagcagagg
tgatagatga 9480gaaagatgac aggccttttg tcctaatcat cggtaacaaa
ggttctacct cgaacagagc 9540aagaacggcc aagaatatac ggctgtacaa
aggaaacaac ccaagagaga tcagggatct 9600gatgagccaa ggaagaatat
tgacggttgc tctaaaagag ttggacccgg aattaaaaga 9660attagtagat
tacaagggga cctttctcaa tagggaagct ttagaagccc taagcttagg
9720taagccaatc aagaggaaaa ccacaacagc aatgatcagg aggttaatag
agccagaggt 9780tgaggaggaa ctaccagatt ggttccaagc ggaagaaccc
ctatttttgg aagcaaaaat 9840acagaatgac ttataccacc taattggcag
tgtagatagt ataaaaagca aagcaaagga 9900attaggggcc acagataaca
caaagatagt gaaggaagtt ggggctagga cctatacgat 9960gaaattgagc
agctggagca cacaagttac aaaaaaacag atgagtctag cccctctctt
10020tgaagagctg ttattaaagt gccctccatg tagtaaaatt tcaaagggac
atatggtgtc 10080agcataccaa ctggctcaag gaaactggga acccctcggg
tgtggggtct atatgggaac 10140cataccagct aggcgtctca agatccaccc
ttatgaggct taccttaaac tcaaagagct 10200ggtggaagtt gaatcttcga
gggccactgc aaaagaatcc atcataagag aacataacac 10260ctggatcctg
cggaaggtga gacatgaagg gaacctaaga accaaatcaa tgatcaaccc
10320tgggaaaata tcagatcagc tatgcagaga tggacacaaa agaaacatat
ataataagat 10380cataggctca acaatggcct ctgctggtat taggctggag
aaactgccag tagtccgagc 10440ccaaactgac acaaccagtt tccaccaagc
cataagagaa aaaattgata aaacagaaaa 10500caagcagacc cctgaattgc
atgaagaact aatgaaggtc ttcgactgct taaagatccc 10560agagctgaag
gaatcgtatg atgaagtttc atgggaacaa ttagaagccg ggataaaccg
10620taagggtgca gcaggctatc tagagagcaa gaacataggg gaagtcctag
acacagagaa 10680acacatagta gagcagctga tcaaggatct gaggaagggg
aagaagatta ggtactatga 10740aacagccatc cccaagaatg agaagagaga
cgtcagcgac gactgggaag ccggagagtt 10800cgttgatgaa aagaaaccaa
gagtaatcca gtacccggac gccaaggtga gactggccat 10860tacaaaagtg
atgtacaaat gggtaaagca aaaaccagtg gtgatacccg gctatgaagg
10920taaaacacct ctatttgaca tattcaacaa agtgaagaag gaatgggatt
cattccagga 10980ccccgtagca gtgagctttg acaccaaagc gtgggataca
caagtcacca gtagagacct 11040aatgttgata aaggatatcc agaaatatta
tttcaagaga agtatacaca aatttttaga 11100tacaataaca gaacacatgg
tggaggtacc tgtcattaca gcagacggtg aagtttacat 11160aaggaatggt
cagaggggta gtggccaacc cgacacaagt gctggtaata gtatgttgaa
11220tgtcctaacc atgatatatg ctttctgtaa aagtacaggc ataccttaca
ggggattcag 11280cagagtggca agaatccatg tgtgtggtga tgatggcttt
ttgataacag agagaggact 11340gggactgaaa ttctctgaga agggtatgca
gatattacat gaggccggga agccccagaa 11400aataactgaa ggggacaaaa
tgaaagtggc atacagattc gaggacatag agttttgttc 11460ccatactccc
gtgccagtca gatgggcaga taacaccagt agttacatgg cagggaggag
11520cacagccact atactagcta agatggcaac caggctggat tccagcggag
agaggggtag 11580cacagcttat gagaaggccg tagccttcag cttccttttg
atgtactcat ggaatcccgt 11640agttagaagg atctgcttac tggtgttgtc
acagtttcca gaaatatccc catccaaaaa 11700cacaatatac tactaccaag
gggatcccat agctgcgtac agagaagtga tagggaaaca 11760gctgtgtgaa
ctgaaaagaa caggatttga gaagctggct ggtctgaatt tgagtatgac
11820cactctaggc atctggacaa aacatactag taaaagacta atccaagcct
gtgtagaaat 11880aggtaagaga gaaggtacct ggttagttaa tgctgacaga
ctgattgcag gaaagactgg 11940gaagttttac atcccaagca ctggtgtcac
tctgttggga aaacactatg aggaaattaa 12000cttaaagcaa aaggcggcac
aaccgccgat agagggggtt gacagatata agttgggccc 12060catagttaat
gttatcttga gaaggctgag ggtgatgctg atgacagttg ccagcggaag
12120ctggtgaatc cgtccggagc gtcgtgccct cactcaaggt ttttaattgt
aaatattgta 12180aatagacagc taagatattt attgtagttg gatagtaatg
cagtgatagt aaatacccca 12240atttaacact acctccaatg cactaagcac
tttagctgtg tgaggttaac tcgacgtcca 12300cggttggact agggaagacc
tctaacagcc cc 123321111840DNAArtificial SequenceXIKE-C-NdN
BVDV-Sequence 11gtatacgaga ttagctaaag aactcgtata tggattggac
gtcaacaaat ttttaattgg 60caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag
gctagccatg cccttagtag 120gactagcaaa agtaggggac tagcggtagc
agtgagttcg ttggatggcc gaacccctga 180gtacagggga gtcgtcaatg
gttcgacact ccattagtcg aggagtctcg agatgccatg 240tggacgaggg
catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg
300ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc
tattccgcta 360gtaaaaactc tgctgtacat ggcacatgga gttgttttcc
gatgaaggga gcaagggtgc 420tacaagtaag aagcagccta agccagatag
gatagaaaaa ggtaagatga aaatagcccc 480aaaagagaca gaaaaagatt
gcaaaaccag accccccgac gcgactatag tagtagaagg 540ggttaagtac
caggtgaaga aaaaaggaaa ggtaagggga aaaaatactc aagatgggtt
600atatcacaac aagaataagc cccctgaatc aagaaaaaaa ttggaaaagg
cactgctggc 660ttgggccatc ttagcagcgg tcctgcttca gctggtaaca
ggagagaata tcacccagtg 720gaacttgatg gacaacggca ccgagggaat
acagcaagcg atgttcctaa gaggggtgaa 780caggagtcta ttaggaattt
ggccagagaa aatttgcacc ggagtaccaa ctcacttagc 840aacagactat
gagcttaaag agatagtggg gatgatggac gcgagtgaga agaccaacta
900cacgtgttgc aggttgcaaa gacatgagtg gaataaacat ggttggtgta
actggtttca 960tatagaaccg tggatatggt tgatgaacaa aacccaaaac
aacctgacag aagggcaacc 1020gcttagggag tgtgctgtga cttgtaggta
tgacaaggaa acagaattga acatcgtgac 1080acaggctagg gacagaccta
caactctgac aggttgcaag aaaggcaaga atttctcttt 1140cgcaggtgtt
atactggatg ggccctgtaa ctttaaagta tcggttgaag atgtgctgtt
1200caaggagcac gattgcggca acatgctgca agagaccgcg atacagctac
tcgatggggc 1260aaccaacacc attgagggag caagggtagg gacggccaag
ttgacaacct ggttagggaa 1320gcaattaggg atccttggta agaagttgga
gaacaaaagc aaagcatggt ttggtgcaca 1380tgcagcaagt ccatactgcg
gagtggagag gaagatcggt tacgtatggt atacaaaaaa 1440ctgcactcca
gcttgccttc caagaaacac tagaataata ggccccggga aatttgatac
1500caacgccgaa gatggaaaaa tactccatga gatggggggg cacctctcag
aatttgtcct 1560attgtccttg gtggttctgt ctgactttgc cccggaaacc
gcgagcgtca tctacttggt 1620tctacatttt gcgatcccgc aaagccacgt
tgatgtagac acatgcgaca agaaccagct 1680gaatttaacg gtagcaacca
cagtagcaga ggtcatacca gggacagtgt ggaacctagg 1740gaagtatgtc
tgcataagac cagactggtg gccatatgag acgacgacag tcttcgtcat
1800agaggaagca gggcaagtaa tcaaattgat gctaagggcc atcagagact
taactaggat 1860atggaatgct gccactacca cagctttctt aatcttttta
gtaaaagcac tgaggggaca 1920actaatccaa gggctattgt ggctgatgct
aataacagga gcacagggct tccctgaatg 1980caaagagggc ttccaatatg
ccatatctaa agacaggaaa atggggttat tggggccaga 2040gagcttaact
acaacatggc acctccccac caaaaaaata gtggattcca tggtgcatgt
2100atggtgtgaa ggaaaagact tgaaaatatt aaaaatgtgc acaaaggaag
agaggtatct 2160agtggctgtg cacgagagag ccttatcaac cagtgccgag
tttatgcaga tcagtgatgg 2220gacaataggc ccagacgtga tagatatgcc
tgatgacttt gagtttggac tctgcccttg 2280tgactcaaaa ccagtgataa
agggcaaatt taatgccagc ttactgaatg gaccagcttt 2340ccagatggta
tgcccacagg ggtggactgg tacaatagaa tgcaccctag cgaaccaaga
2400caccttggac acaactgtca ttaggacata tagaagaact accccatttc
agcggagaaa 2460atggtgtacc tatgaaaaaa taatagggga agatatctat
gaatgcattc taggtggaaa 2520ctggacatgc ataaccggtg accatagcag
gttgaaagac ggacctatca agaagtgtaa 2580gtggtgtggc catgacttcg
tcaactcaga ggggctacca cactacccaa taggcaagtg 2640catgctcatc
aacgagagtg ggtacaggta tgtagatgac acctcttgcg ataggggtgg
2700tgtagccata gttccatctg gcaccgtaaa gtgtagaata ggtaacgtca
cggtgcaagt 2760tatcgctact aacaatgatc tgggacccat gccttgcagc
ccagctgaag tgatagcaag 2820tgaaggacca gtggaaaaga ctgcatgcac
attcaactat tcaaggactc tacctaataa 2880gtattatgag ccaagggacc
ggtacttcca acaatacatg ttaaaagggg agtggcaata 2940ttggttcgac
ctggattctg tagaccacca caaagactac ttctcagagt tcataatcat
3000agcagtggtc gccttgttgg gtggtaagta cgtactgtgg ctcttgataa
catacacaat 3060actgtctgag cagatggcta tgggtgctgg agtgaatact
gaagagatag tcatgatagg 3120caatttgctg acagacagtg atattgaggt
tgtggtttat ttccttcttc tgtacttaat 3180agttaaagag gaactggcga
ggaaatggat tatactggta taccacatcc ttgtagccaa 3240ccctatgaaa
acaattgggg tcgtcttact aatgctaggg ggagtggtga aggccagcag
3300aatcaatgct gatgaccaaa gtgctatgga cccatgcttt cttctcgtga
caggcgtagt 3360ggctgttttg atgatcgcta gaagagaacc tgccacatta
ccactgattg tagcattgct 3420agcaataaga acatcaggat tcctactgcc
cgctagcatt gatgtaactg tagcagtagt 3480attaattgta cttttgttgg
ctagctacat aacagactac tttagatata aaaagtggct 3540tcaactctta
tttagtctga tagctggtat ctttattata aggagcttaa aacatatcaa
3600ccagatggag gtaccagaaa tatctatgcc aagttggaga cctctagctc
tggtcctttt 3660ctatataaca tctacagcaa taaccactaa ttgggacatt
gacttagcag gcttcctgct 3720gcaatgggcg ccagcagtga tcatgatggc
taccatgtgg gcagactttt tgactctgat 3780catagtcctg cccagttacg
agttatctaa gctttacttc ctaaagaacg tcaggacaga 3840cgtggaaaag
aactggctcg gcaaagtgaa atacagacag atcagttcag tttatgacat
3900ctgtgacagt gaggaagcag tgtacctatt tccatcaagg cataagagtg
gaagcaggcc 3960agatttcata ttaccttttt tgaaagccgt gttaataagc
tgcatcagca gccaatggca 4020agtggtttac atttcttacc taatactgga
aattacatac tatatgcaca ggaaaatcat 4080agatgaggtg tcaggaggag
caaattttct atcaagactc atagcagcca tcatagaatt 4140aaattgggcc
atagatgatg aggaatgtaa
aggactgaag aaactgtatc tcttgtcagg 4200gagagcgaag aatttgatag
ttaaacataa ggtaagaaat gaagccgtcc acagatggtt 4260tggtgaggag
gaaatatacg gggcacccaa ggtgatcact atcataaaag ctagtaccct
4320aagtaaaaac aggcactgca taatctgcac gatctgtgaa gggaaagaat
ggaatggagc 4380caactgccca aagtgtggaa gacaaggaaa gcccataaca
tgtggaatga cactcgcaga 4440ctttgaggag aaacattaca aaaagatatt
tataagagaa gaatcttctt gtcctgtgcc 4500ttttgatcct tcttgccatt
gtaattattt tcgccacgat gggcctttca ggaaagagta 4560taagggttac
gtccaataca cagccagagg acaactcttt ctgaggaacc taccaattct
4620agcgacgaag atgaagctat taatggtggg aaacctcggc gcagaaattg
gcgacctgga 4680acatctagga tgggtactga gagggccagc cgtgtgcaaa
aaaattacca accatgagaa 4740gtgccacgta aacatcatgg ataagctaac
tgcatttttt ggaatcatgc ctagaggcac 4800gacccctagg gcacctgtga
ggttccccac agcactacta aaagtgagaa gggggctaga 4860gacgggatgg
gcttacacgc accaaggagg gatcagctcg gtagaccatg tcacagccgg
4920aaaggattta ctagtgtgtg acagtatggg caggaccagg gttgtctgtc
atagtaacaa 4980taagatgact gatgagactg agtatggcat caagaccgac
tcagggtgtc ccgaaggtgc 5040gaggtgttac gtgctaaacc cagaagctgt
taacatttct ggcacaaaag gagctatggt 5100acacctccag aaaacggggg
gggagttcac atgtgtcact gcctcaggga ccccggcttt 5160cttcgatctg
aaaaatctaa aaggctggtc cgggctacca atttttgaag catccagtgg
5220cagggtggtt ggtagggtga aagtcggcaa gaatgaggat tccaagccca
ccaaactaat 5280gagcggaatc cagacagtgt ctaagaacca gacagaccta
gcggacatcg taaaaaaatt 5340gactagtatg aacagaggag agttcaaaca
gataacatta gccactgggg caggaaaaac 5400tacggaactg ccaaggtccg
tcatagagga gatagggagg cacaaaaggg tcttagtcct 5460gataccattg
agagcagcag cagagtcagt gtatcagtat atgagagtga agtacccaag
5520tatatctttc aatttgagaa taggagatat gaaggaaggt gacatggcca
ctggtatcac 5580ctacgcctca tatgggtact tttgtcagct tcctcagccc
aaactgagag ctgccatggt 5640agagtactca tatatattct tagatgagta
ccactgtgct acacccgagc aattagcaat 5700aattggaaag atacacaggt
ttgctgaaaa tcttagagtg gtagcaatga cagcaacccc 5760agctggaacg
gtcacaacga ctggtcagaa acaccctata gaggagttca tagccccaga
5820ggtgatgaaa ggtgaagatc taggtagtga atacttggat attgcagggt
tgaagatacc 5880gactgaagag atgaaaggca acatgctcgt gttcgcgcca
actaggaaca tggcagtaga 5940aacagctaag aaattgaagg ctaagggata
caactctgga tactattaca gtggggaaaa 6000cccagagaac ttgagggtgg
taacctcgca atccccgtat gtggtagtag ccaccaatgc 6060catagagtca
ggtgtgacat taccagactt agacacagtt gtagacactg gactaaagtg
6120tgagaagagg gtgaggattt cttcaaaaat gcccttcatt gtaacaggac
ttaagagaat 6180ggcagtcaca atcggagagc aagcccagcg caggggtaga
gtaggaagag tcaagccagg 6240taggtactat aggagtcaag aaacagcttc
agggtcaaaa gattaccatt acgacctact 6300gcaagcccag aggtacggaa
tagaagatgg aattaatgta acaaagtcat tcagggagat 6360gaactatgat
tggagccttt acgaagagga cagcttgatg ataactcaac tcgaggtcct
6420taacaacctc cttatatcag aagacctgcc tgccgcagtg aagaacatca
tggcccggac 6480cgatcaccca gaacccatac aactggccta taacagttat
gaaaaccaaa ttccagtgct 6540gttcccaaag atcaaaaatg gtgaggtgac
agacagttat gagaattaca catatctcaa 6600tgcaagaaaa ttaggagagg
acgtgccggc atatgtgtac gccacagagg atgaggatct 6660agcagtggat
cttctgggta tggattggcc ggacccaggc aaccaacagg tggtagagac
6720agggagggca ttaaaacaag taactggctt atccacagca gaaaacgccc
tcttgatagc 6780cctattcggc tacgtcgggt accagacact ttcaaaaagg
cacataccca tgattactga 6840catctataca cttgaagacc acaggcttga
ggacacaacc cacctccagt ttgccccaaa 6900cgctataagg accgacggca
aggactcaga gttgaaggaa ttagctgtgg gagaccttga 6960taaatatgtg
gacgcactgg tagactactc caaacaaggg atgaaattca tcaaagtcca
7020agctgaaaag gtcagagact cccagtctac gaaggaaggc ttgcaaacca
ttaaggagta 7080tgtggataag tttatacaat cactaacaga gaataaggag
gagatcatca ggtatggact 7140atggggagtt cacacggcac tctacaaaag
cttggcagcg agactggggc atgaaacagc 7200ttttgcaact ttagtggtaa
aatggttggc ttttgggggc gaaacggtat ctgctcacat 7260caagcaagta
gcagttgatc tagtagtata ttatatcatc aacaaaccat cttttcctgg
7320agatacagag acccaacaag aggggaggaa gtttgtggct agtcttttta
tatctgcact 7380agcaacatac acatataaaa cctggaatta caacaatctg
caacgggttg tcgaacctgc 7440cttagcttac ctcccatatg ctacaagtgc
cttgaagttg ttcacaccca caagattaga 7500gagtgtggtc atactcagtt
ctacaattta caagacatac ctctctataa ggaagggtaa 7560gagtgacggc
ttgttaggta caggcataag tgcagccatg gagatcttaa accaaaaccc
7620aatctcagta ggtatatctg tgatgctggg ggtaggtgcc atcgccgccc
ataatgcaat 7680agaatctagt gaacagaaaa gaactttgct gatgaaggtc
tttgtaaaaa acttcttaga 7740ccaagcagca acagatgagc tagtcaaaga
gaaccctgaa aaaataatca tggctctatt 7800tgaagcagtc cagaccatag
gaaaccccct aagactcatc taccatctgt acggggtgta 7860ctataagggg
tgggaagcaa aagaactcgc agagaaaact gctggccgca acttattcac
7920attgatcatg tttgaggcct ttgagctttt aggtatggac tcagaaggaa
agataagaaa 7980cttgtcaggc aactacatac tggacttaat cttcaacttg
cataataaat taaacaaggg 8040gctcaaaaaa ctagtccttg ggtgggctcc
tgcacctttg agctgtgatt ggacaccaag 8100tgatgagaga ataagcctac
ctcataacaa ctacttaagg gtagaaacca ggtgtccttg 8160tggctatgag
atgaaggcaa taaaaaatgt tgctggtaaa ttgacaaaag ttgaagaaaa
8220ggggtccttc ctatgcagga atagattagg gagaggacct ccaaacttca
aagtaacaaa 8280gttctatgat gataacttga tagaagtcaa gccagtagct
aggctagaag gccaggtgga 8340cctctattac aagggagtaa cagctaagtt
agactacaac aatgggaaag tactgttagc 8400taccaacaag tgggaggtgg
accacgcttt cctgaccaga ctagtaaaga agcacacagg 8460gataggtttt
aaaggtgcat atttgggtga ccgaccagac catcaagatc ttgtcgatag
8520agattgtgca actataacga agaactcagt acagttccta aaaatgaaga
agggttgcgc 8580tttcacatat gacctaacaa tctctaacct tgtcaggctt
attgaactag tccataagaa 8640taatttacaa gaaagagaga tccctaccgt
gacagtaact acttggcttg catattcttt 8700tgtcaatgaa gacctgggga
ctatcaagcc tgtattgggg gagaaagtca tcccagaacc 8760ccccgaggag
ttgagtctcc aacccaccgt gagactagtc accactgaaa cagcaataac
8820cataacaggg gaggctgaag tgatgacgac agggatcaca ccagtggtag
agatgaaaga 8880agaacctcag ctggaccacc agtcaactac cctaaaggta
gggttgaagg aaggggaata 8940tccagggcca ggagttaacc ctaaccattt
agcagaggtg atagatgaga aagatgacag 9000gccttttgtc ctaatcatcg
gtaacaaagg ttctacctcg aacagagcaa gaacggccaa 9060gaatatacgg
ctgtacaaag gaaacaaccc aagagagatc agggatctga tgagccaagg
9120aagaatattg acggttgctc taaaagagtt ggacccggaa ttaaaagaat
tagtagatta 9180caaggggacc tttctcaata gggaagcttt agaagcccta
agcttaggta agccaatcaa 9240gaggaaaacc acaacagcaa tgatcaggag
gttaatagag ccagaggttg aggaggaact 9300accagattgg ttccaagcgg
aagaacccct atttttggaa gcaaaaatac agaatgactt 9360ataccaccta
attggcagtg tagatagtat aaaaagcaaa gcaaaggaat taggggccac
9420agataacaca aagatagtga aggaagttgg ggctaggacc tatacgatga
aattgagcag 9480ctggagcaca caagttacaa aaaaacagat gagtctagcc
cctctctttg aagagctgtt 9540attaaagtgc cctccatgta gtaaaatttc
aaagggacat atggtgtcag cataccaact 9600ggctcaagga aactgggaac
ccctcgggtg tggggtctat atgggaacca taccagctag 9660gcgtctcaag
atccaccctt atgaggctta ccttaaactc aaagagctgg tggaagttga
9720atcttcgagg gccactgcaa aagaatccat cataagagaa cataacacct
ggatcctgcg 9780gaaggtgaga catgaaggga acctaagaac caaatcaatg
atcaaccctg ggaaaatatc 9840agatcagcta tgcagagatg gacacaaaag
aaacatatat aataagatca taggctcaac 9900aatggcctct gctggtatta
ggctggagaa actgccagta gtccgagccc aaactgacac 9960aaccagtttc
caccaagcca taagagaaaa aattgataaa acagaaaaca agcagacccc
10020tgaattgcat gaagaactaa tgaaggtctt cgactgctta aagatcccag
agctgaagga 10080atcgtatgat gaagtttcat gggaacaatt agaagccggg
ataaaccgta agggtgcagc 10140aggctatcta gagagcaaga acatagggga
agtcctagac acagagaaac acatagtaga 10200gcagctgatc aaggatctga
ggaaggggaa gaagattagg tactatgaaa cagccatccc 10260caagaatgag
aagagagacg tcagcgacga ctgggaagcc ggagagttcg ttgatgaaaa
10320gaaaccaaga gtaatccagt acccggacgc caaggtgaga ctggccatta
caaaagtgat 10380gtacaaatgg gtaaagcaaa aaccagtggt gatacccggc
tatgaaggta aaacacctct 10440atttgacata ttcaacaaag tgaagaagga
atgggattca ttccaggacc ccgtagcagt 10500gagctttgac accaaagcgt
gggatacaca agtcaccagt agagacctaa tgttgataaa 10560ggatatccag
aaatattatt tcaagagaag tatacacaaa tttttagata caataacaga
10620acacatggtg gaggtacctg tcattacagc agacggtgaa gtttacataa
ggaatggtca 10680gaggggtagt ggccaacccg acacaagtgc tggtaatagt
atgttgaatg tcctaaccat 10740gatatatgct ttctgtaaaa gtacaggcat
accttacagg ggattcagca gagtggcaag 10800aatccatgtg tgtggtgatg
atggcttttt gataacagag agaggactgg gactgaaatt 10860ctctgagaag
ggtatgcaga tattacatga ggccgggaag ccccagaaaa taactgaagg
10920ggacaaaatg aaagtggcat acagattcga ggacatagag ttttgttccc
atactcccgt 10980gccagtcaga tgggcagata acaccagtag ttacatggca
gggaggagca cagccactat 11040actagctaag atggcaacca ggctggattc
cagcggagag aggggtagca cagcttatga 11100gaaggccgta gccttcagct
tccttttgat gtactcatgg aatcccgtag ttagaaggat 11160ctgcttactg
gtgttgtcac agtttccaga aatatcccca tccaaaaaca caatatacta
11220ctaccaaggg gatcccatag ctgcgtacag agaagtgata gggaaacagc
tgtgtgaact 11280gaaaagaaca ggatttgaga agctggctgg tctgaatttg
agtatgacca ctctaggcat 11340ctggacaaaa catactagta aaagactaat
ccaagcctgt gtagaaatag gtaagagaga 11400aggtacctgg ttagttaatg
ctgacagact gattgcagga aagactggga agttttacat 11460cccaagcact
ggtgtcactc tgttgggaaa acactatgag gaaattaact taaagcaaaa
11520ggcggcacaa ccgccgatag agggggttga cagatataag ttgggcccca
tagttaatgt 11580tatcttgaga aggctgaggg tgatgctgat gacagttgcc
agcggaagct ggtgaatccg 11640tccggagcgt cgtgccctca ctcaaggttt
ttaattgtaa atattgtaaa tagacagcta 11700agatatttat tgtagttgga
tagtaatgca gtgatagtaa ataccccaat ttaacactac 11760ctccaatgca
ctaagcactt tagctgtgtg aggttaactc gacgtccacg gttggactag
11820ggaagacctc taacagcccc 11840123749PRTArtificial
SequenceXIKE-C-NdN 12Met Glu Leu Phe Ser Asp Glu Gly Ser Lys Gly
Ala Thr Ser Lys Lys 1 5 10 15 Gln Pro Lys Pro Asp Arg Ile Glu Lys
Gly Lys Met Lys Ile Ala Pro 20 25 30 Lys Glu Thr Glu Lys Asp Cys
Lys Thr Arg Pro Pro Asp Ala Thr Ile 35 40 45 Val Val Glu Gly Val
Lys Tyr Gln Val Lys Lys Lys Gly Lys Val Arg 50 55 60 Gly Lys Asn
Thr Gln Asp Gly Leu Tyr His Asn Lys Asn Lys Pro Pro 65 70 75 80 Glu
Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala Trp Ala Ile Leu 85 90
95 Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn Ile Thr Gln Trp
100 105 110 Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln Ala Met
Phe Leu 115 120 125 Arg Gly Val Asn Arg Ser Leu Leu Gly Ile Trp Pro
Glu Lys Ile Cys 130 135 140 Thr Gly Val Pro Thr His Leu Ala Thr Asp
Tyr Glu Leu Lys Glu Ile 145 150 155 160 Val Gly Met Met Asp Ala Ser
Glu Lys Thr Asn Tyr Thr Cys Cys Arg 165 170 175 Leu Gln Arg His Glu
Trp Asn Lys His Gly Trp Cys Asn Trp Phe His 180 185 190 Ile Glu Pro
Trp Ile Trp Leu Met Asn Lys Thr Gln Asn Asn Leu Thr 195 200 205 Glu
Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys Arg Tyr Asp Lys 210 215
220 Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp Arg Pro Thr Thr
225 230 235 240 Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe Ala
Gly Val Ile 245 250 255 Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val
Glu Asp Val Leu Phe 260 265 270 Lys Glu His Asp Cys Gly Asn Met Leu
Gln Glu Thr Ala Ile Gln Leu 275 280 285 Leu Asp Gly Ala Thr Asn Thr
Ile Glu Gly Ala Arg Val Gly Thr Ala 290 295 300 Lys Leu Thr Thr Trp
Leu Gly Lys Gln Leu Gly Ile Leu Gly Lys Lys 305 310 315 320 Leu Glu
Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala Ala Ser Pro 325 330 335
Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp Tyr Thr Lys Asn 340
345 350 Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile Ile Gly Pro
Gly 355 360 365 Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu His
Glu Met Gly 370 375 380 Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu
Val Val Leu Ser Asp 385 390 395 400 Phe Ala Pro Glu Thr Ala Ser Val
Ile Tyr Leu Val Leu His Phe Ala 405 410 415 Ile Pro Gln Ser His Val
Asp Val Asp Thr Cys Asp Lys Asn Gln Leu 420 425 430 Asn Leu Thr Val
Ala Thr Thr Val Ala Glu Val Ile Pro Gly Thr Val 435 440 445 Trp Asn
Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp Trp Pro Tyr 450 455 460
Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly Gln Val Ile Lys 465
470 475 480 Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp Asn
Ala Ala 485 490 495 Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala
Leu Arg Gly Gln 500 505 510 Leu Ile Gln Gly Leu Leu Trp Leu Met Leu
Ile Thr Gly Ala Gln Gly 515 520 525 Phe Pro Glu Cys Lys Glu Gly Phe
Gln Tyr Ala Ile Ser Lys Asp Arg 530 535 540 Lys Met Gly Leu Leu Gly
Pro Glu Ser Leu Thr Thr Thr Trp His Leu 545 550 555 560 Pro Thr Lys
Lys Ile Val Asp Ser Met Val His Val Trp Cys Glu Gly 565 570 575 Lys
Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu Glu Arg Tyr Leu 580 585
590 Val Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala Glu Phe Met Gln
595 600 605 Ile Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp Met Pro
Asp Asp 610 615 620 Phe Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro
Val Ile Lys Gly 625 630 635 640 Lys Phe Asn Ala Ser Leu Leu Asn Gly
Pro Ala Phe Gln Met Val Cys 645 650 655 Pro Gln Gly Trp Thr Gly Thr
Ile Glu Cys Thr Leu Ala Asn Gln Asp 660 665 670 Thr Leu Asp Thr Thr
Val Ile Arg Thr Tyr Arg Arg Thr Thr Pro Phe 675 680 685 Gln Arg Arg
Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly Glu Asp Ile 690 695 700 Tyr
Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile Thr Gly Asp His 705 710
715 720 Ser Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys Trp Cys Gly
His 725 730 735 Asp Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro Ile
Gly Lys Cys 740 745 750 Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val
Asp Asp Thr Ser Cys 755 760 765 Asp Arg Gly Gly Val Ala Ile Val Pro
Ser Gly Thr Val Lys Cys Arg 770 775 780 Ile Gly Asn Val Thr Val Gln
Val Ile Ala Thr Asn Asn Asp Leu Gly 785 790 795 800 Pro Met Pro Cys
Ser Pro Ala Glu Val Ile Ala Ser Glu Gly Pro Val 805 810 815 Glu Lys
Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr Leu Pro Asn Lys 820 825 830
Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr Met Leu Lys Gly 835
840 845 Glu Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val Asp His His Lys
Asp 850 855 860 Tyr Phe Ser Glu Phe Ile Ile Ile Ala Val Val Ala Leu
Leu Gly Gly 865 870 875 880 Lys Tyr Val Leu Trp Leu Leu Ile Thr Tyr
Thr Ile Leu Ser Glu Gln 885 890 895 Met Ala Met Gly Ala Gly Val Asn
Thr Glu Glu Ile Val Met Ile Gly 900 905 910 Asn Leu Leu Thr Asp Ser
Asp Ile Glu Val Val Val Tyr Phe Leu Leu 915 920 925 Leu Tyr Leu Ile
Val Lys Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu 930 935 940 Val Tyr
His Ile Leu Val Ala Asn Pro Met Lys Thr Ile Gly Val Val 945 950 955
960 Leu Leu Met Leu Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp
965 970 975 Asp Gln Ser Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly
Val Val 980 985 990 Ala Val Leu Met Ile Ala Arg Arg Glu Pro Ala Thr
Leu Pro Leu Ile 995 1000 1005 Val Ala Leu Leu Ala Ile Arg Thr Ser
Gly Phe Leu Leu Pro Ala 1010 1015 1020 Ser Ile Asp Val Thr Val Ala
Val Val Leu Ile Val Leu Leu Leu 1025 1030 1035 Ala Ser Tyr Ile Thr
Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln 1040 1045 1050 Leu Leu Phe
Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu 1055 1060 1065 Lys
His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser 1070 1075
1080 Trp Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala
1085 1090 1095 Ile Thr Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu
Leu Gln 1100 1105 1110
Trp Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe 1115
1120 1125 Leu Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys
Leu 1130 1135 1140 Tyr Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys
Asn Trp Leu 1145 1150 1155 Gly Lys Val Lys Tyr Arg Gln Ile Ser Ser
Val Tyr Asp Ile Cys 1160 1165 1170 Asp Ser Glu Glu Ala Val Tyr Leu
Phe Pro Ser Arg His Lys Ser 1175 1180 1185 Gly Ser Arg Pro Asp Phe
Ile Leu Pro Phe Leu Lys Ala Val Leu 1190 1195 1200 Ile Ser Cys Ile
Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr 1205 1210 1215 Leu Ile
Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp 1220 1225 1230
Glu Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala 1235
1240 1245 Ile Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys
Gly 1250 1255 1260 Leu Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys
Asn Leu Ile 1265 1270 1275 Val Lys His Lys Val Arg Asn Glu Ala Val
His Arg Trp Phe Gly 1280 1285 1290 Glu Glu Glu Ile Tyr Gly Ala Pro
Lys Val Ile Thr Ile Ile Lys 1295 1300 1305 Ala Ser Thr Leu Ser Lys
Asn Arg His Cys Ile Ile Cys Thr Ile 1310 1315 1320 Cys Glu Gly Lys
Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly 1325 1330 1335 Arg Gln
Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe 1340 1345 1350
Glu Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser 1355
1360 1365 Cys Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe
Arg 1370 1375 1380 His Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr
Val Gln Tyr 1385 1390 1395 Thr Ala Arg Gly Gln Leu Phe Leu Arg Asn
Leu Pro Ile Leu Ala 1400 1405 1410 Thr Lys Met Lys Leu Leu Met Val
Gly Asn Leu Gly Ala Glu Ile 1415 1420 1425 Gly Asp Leu Glu His Leu
Gly Trp Val Leu Arg Gly Pro Ala Val 1430 1435 1440 Cys Lys Lys Ile
Thr Asn His Glu Lys Cys His Val Asn Ile Met 1445 1450 1455 Asp Lys
Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr 1460 1465 1470
Pro Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg 1475
1480 1485 Arg Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly
Ile 1490 1495 1500 Ser Ser Val Asp His Val Thr Ala Gly Lys Asp Leu
Leu Val Cys 1505 1510 1515 Asp Ser Met Gly Arg Thr Arg Val Val Cys
His Ser Asn Asn Lys 1520 1525 1530 Met Thr Asp Glu Thr Glu Tyr Gly
Ile Lys Thr Asp Ser Gly Cys 1535 1540 1545 Pro Glu Gly Ala Arg Cys
Tyr Val Leu Asn Pro Glu Ala Val Asn 1550 1555 1560 Ile Ser Gly Thr
Lys Gly Ala Met Val His Leu Gln Lys Thr Gly 1565 1570 1575 Gly Glu
Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe 1580 1585 1590
Asp Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu 1595
1600 1605 Ala Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys
Asn 1610 1615 1620 Glu Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile
Gln Thr Val 1625 1630 1635 Ser Lys Asn Gln Thr Asp Leu Ala Asp Ile
Val Lys Lys Leu Thr 1640 1645 1650 Ser Met Asn Arg Gly Glu Phe Lys
Gln Ile Thr Leu Ala Thr Gly 1655 1660 1665 Ala Gly Lys Thr Thr Glu
Leu Pro Arg Ser Val Ile Glu Glu Ile 1670 1675 1680 Gly Arg His Lys
Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala 1685 1690 1695 Ala Glu
Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile 1700 1705 1710
Ser Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala 1715
1720 1725 Thr Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu
Pro 1730 1735 1740 Gln Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser
Tyr Ile Phe 1745 1750 1755 Leu Asp Glu Tyr His Cys Ala Thr Pro Glu
Gln Leu Ala Ile Ile 1760 1765 1770 Gly Lys Ile His Arg Phe Ala Glu
Asn Leu Arg Val Val Ala Met 1775 1780 1785 Thr Ala Thr Pro Ala Gly
Thr Val Thr Thr Thr Gly Gln Lys His 1790 1795 1800 Pro Ile Glu Glu
Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp 1805 1810 1815 Leu Gly
Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr 1820 1825 1830
Glu Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn 1835
1840 1845 Met Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr
Asn 1850 1855 1860 Ser Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn
Leu Arg Val 1865 1870 1875 Val Thr Ser Gln Ser Pro Tyr Val Val Val
Ala Thr Asn Ala Ile 1880 1885 1890 Glu Ser Gly Val Thr Leu Pro Asp
Leu Asp Thr Val Val Asp Thr 1895 1900 1905 Gly Leu Lys Cys Glu Lys
Arg Val Arg Ile Ser Ser Lys Met Pro 1910 1915 1920 Phe Ile Val Thr
Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu 1925 1930 1935 Gln Ala
Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg 1940 1945 1950
Tyr Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His 1955
1960 1965 Tyr Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly
Ile 1970 1975 1980 Asn Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp
Trp Ser Leu 1985 1990 1995 Tyr Glu Glu Asp Ser Leu Met Ile Thr Gln
Leu Glu Val Leu Asn 2000 2005 2010 Asn Leu Leu Ile Ser Glu Asp Leu
Pro Ala Ala Val Lys Asn Ile 2015 2020 2025 Met Ala Arg Thr Asp His
Pro Glu Pro Ile Gln Leu Ala Tyr Asn 2030 2035 2040 Ser Tyr Glu Asn
Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn 2045 2050 2055 Gly Glu
Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala 2060 2065 2070
Arg Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu 2075
2080 2085 Asp Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro
Asp 2090 2095 2100 Pro Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala
Leu Lys Gln 2105 2110 2115 Val Thr Gly Leu Ser Thr Ala Glu Asn Ala
Leu Leu Ile Ala Leu 2120 2125 2130 Phe Gly Tyr Val Gly Tyr Gln Thr
Leu Ser Lys Arg His Ile Pro 2135 2140 2145 Met Ile Thr Asp Ile Tyr
Thr Leu Glu Asp His Arg Leu Glu Asp 2150 2155 2160 Thr Thr His Leu
Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly 2165 2170 2175 Lys Asp
Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys 2180 2185 2190
Tyr Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe 2195
2200 2205 Ile Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr
Lys 2210 2215 2220 Glu Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys
Phe Ile Gln 2225 2230 2235 Ser Leu Thr Glu Asn Lys Glu Glu Ile Ile
Arg Tyr Gly Leu Trp 2240 2245 2250 Gly Val His Thr Ala Leu Tyr Lys
Ser Leu Ala Ala Arg Leu Gly 2255 2260 2265 His Glu Thr Ala Phe Ala
Thr Leu Val Val Lys Trp Leu Ala Phe 2270 2275 2280 Gly Gly Glu Thr
Val Ser Ala His Ile Lys Gln Val Ala Val Asp 2285 2290 2295 Leu Val
Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp 2300 2305 2310
Thr Glu Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe 2315
2320 2325 Ile Ser Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr
Asn 2330 2335 2340 Asn Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr
Leu Pro Tyr 2345 2350 2355 Ala Thr Ser Ala Leu Lys Leu Phe Thr Pro
Thr Arg Leu Glu Ser 2360 2365 2370 Val Val Ile Leu Ser Ser Thr Ile
Tyr Lys Thr Tyr Leu Ser Ile 2375 2380 2385 Arg Lys Gly Lys Ser Asp
Gly Leu Leu Gly Thr Gly Ile Ser Ala 2390 2395 2400 Ala Met Glu Ile
Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser 2405 2410 2415 Val Met
Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu 2420 2425 2430
Ser Ser Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys 2435
2440 2445 Asn Phe Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu
Asn 2450 2455 2460 Pro Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val
Gln Thr Ile 2465 2470 2475 Gly Asn Pro Leu Arg Leu Ile Tyr His Leu
Tyr Gly Val Tyr Tyr 2480 2485 2490 Lys Gly Trp Glu Ala Lys Glu Leu
Ala Glu Lys Thr Ala Gly Arg 2495 2500 2505 Asn Leu Phe Thr Leu Ile
Met Phe Glu Ala Phe Glu Leu Leu Gly 2510 2515 2520 Met Asp Ser Glu
Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile 2525 2530 2535 Leu Asp
Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu 2540 2545 2550
Lys Lys Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp 2555
2560 2565 Trp Thr Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn
Tyr 2570 2575 2580 Leu Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu
Met Lys Ala 2585 2590 2595 Ile Lys Asn Val Ala Gly Lys Leu Thr Lys
Val Glu Glu Lys Gly 2600 2605 2610 Ser Phe Leu Cys Arg Asn Arg Leu
Gly Arg Gly Pro Pro Asn Phe 2615 2620 2625 Lys Val Thr Lys Phe Tyr
Asp Asp Asn Leu Ile Glu Val Lys Pro 2630 2635 2640 Val Ala Arg Leu
Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val 2645 2650 2655 Thr Ala
Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr 2660 2665 2670
Asn Lys Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys 2675
2680 2685 Lys His Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp
Arg 2690 2695 2700 Pro Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala
Thr Ile Thr 2705 2710 2715 Lys Asn Ser Val Gln Phe Leu Lys Met Lys
Lys Gly Cys Ala Phe 2720 2725 2730 Thr Tyr Asp Leu Thr Ile Ser Asn
Leu Val Arg Leu Ile Glu Leu 2735 2740 2745 Val His Lys Asn Asn Leu
Gln Glu Arg Glu Ile Pro Thr Val Thr 2750 2755 2760 Val Thr Thr Trp
Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly 2765 2770 2775 Thr Ile
Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro 2780 2785 2790
Glu Glu Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu 2795
2800 2805 Thr Ala Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr
Gly 2810 2815 2820 Ile Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln
Leu Asp His 2825 2830 2835 Gln Ser Thr Thr Leu Lys Val Gly Leu Lys
Glu Gly Glu Tyr Pro 2840 2845 2850 Gly Pro Gly Val Asn Pro Asn His
Leu Ala Glu Val Ile Asp Glu 2855 2860 2865 Lys Asp Asp Arg Pro Phe
Val Leu Ile Ile Gly Asn Lys Gly Ser 2870 2875 2880 Thr Ser Asn Arg
Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys 2885 2890 2895 Gly Asn
Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg 2900 2905 2910
Ile Leu Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu 2915
2920 2925 Leu Val Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu
Glu 2930 2935 2940 Ala Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr
Thr Thr Ala 2945 2950 2955 Met Ile Arg Arg Leu Ile Glu Pro Glu Val
Glu Glu Glu Leu Pro 2960 2965 2970 Asp Trp Phe Gln Ala Glu Glu Pro
Leu Phe Leu Glu Ala Lys Ile 2975 2980 2985 Gln Asn Asp Leu Tyr His
Leu Ile Gly Ser Val Asp Ser Ile Lys 2990 2995 3000 Ser Lys Ala Lys
Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val 3005 3010 3015 Lys Glu
Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp 3020 3025 3030
Ser Thr Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe 3035
3040 3045 Glu Glu Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser
Lys 3050 3055 3060 Gly His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly
Asn Trp Glu 3065 3070 3075 Pro Leu Gly Cys Gly Val Tyr Met Gly Thr
Ile Pro Ala Arg Arg 3080 3085 3090 Leu Lys Ile His Pro Tyr Glu Ala
Tyr Leu Lys Leu Lys Glu Leu 3095 3100 3105 Val Glu Val Glu Ser Ser
Arg Ala Thr Ala Lys Glu Ser Ile Ile 3110 3115 3120 Arg Glu His Asn
Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly 3125 3130 3135 Asn Leu
Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp 3140 3145 3150
Gln Leu Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile 3155
3160 3165 Ile Gly Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys
Leu 3170 3175 3180 Pro Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe
His Gln Ala 3185 3190 3195 Ile Arg Glu Lys Ile Asp Lys Thr Glu Asn
Lys Gln Thr Pro Glu 3200 3205 3210 Leu His Glu Glu Leu Met Lys Val
Phe Asp Cys Leu Lys Ile Pro 3215 3220 3225 Glu Leu Lys Glu Ser Tyr
Asp Glu Val Ser Trp Glu Gln Leu Glu 3230 3235 3240 Ala Gly Ile Asn
Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys 3245 3250 3255 Asn Ile
Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln 3260 3265 3270
Leu Ile Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu 3275
3280 3285 Thr Ala Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp
Trp 3290 3295 3300 Glu Ala Gly Glu Phe Val Asp Glu Lys Lys Pro
Arg
Val Ile Gln 3305 3310 3315 Tyr Pro Asp Ala Lys Val Arg Leu Ala Ile
Thr Lys Val Met Tyr 3320 3325 3330 Lys Trp Val Lys Gln Lys Pro Val
Val Ile Pro Gly Tyr Glu Gly 3335 3340 3345 Lys Thr Pro Leu Phe Asp
Ile Phe Asn Lys Val Lys Lys Glu Trp 3350 3355 3360 Asp Ser Phe Gln
Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala 3365 3370 3375 Trp Asp
Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp 3380 3385 3390
Ile Gln Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp 3395
3400 3405 Thr Ile Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala
Asp 3410 3415 3420 Gly Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser
Gly Gln Pro 3425 3430 3435 Asp Thr Ser Ala Gly Asn Ser Met Leu Asn
Val Leu Thr Met Ile 3440 3445 3450 Tyr Ala Phe Cys Lys Ser Thr Gly
Ile Pro Tyr Arg Gly Phe Ser 3455 3460 3465 Arg Val Ala Arg Ile His
Val Cys Gly Asp Asp Gly Phe Leu Ile 3470 3475 3480 Thr Glu Arg Gly
Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln 3485 3490 3495 Ile Leu
His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp 3500 3505 3510
Lys Met Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser 3515
3520 3525 His Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser
Tyr 3530 3535 3540 Met Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys
Met Ala Thr 3545 3550 3555 Arg Leu Asp Ser Ser Gly Glu Arg Gly Ser
Thr Ala Tyr Glu Lys 3560 3565 3570 Ala Val Ala Phe Ser Phe Leu Leu
Met Tyr Ser Trp Asn Pro Val 3575 3580 3585 Val Arg Arg Ile Cys Leu
Leu Val Leu Ser Gln Phe Pro Glu Ile 3590 3595 3600 Ser Pro Ser Lys
Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile 3605 3610 3615 Ala Ala
Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys 3620 3625 3630
Arg Thr Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr 3635
3640 3645 Thr Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile
Gln 3650 3655 3660 Ala Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp
Leu Val Asn 3665 3670 3675 Ala Asp Arg Leu Ile Ala Gly Lys Thr Gly
Lys Phe Tyr Ile Pro 3680 3685 3690 Ser Thr Gly Val Thr Leu Leu Gly
Lys His Tyr Glu Glu Ile Asn 3695 3700 3705 Leu Lys Gln Lys Ala Ala
Gln Pro Pro Ile Glu Gly Val Asp Arg 3710 3715 3720 Tyr Lys Leu Gly
Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg 3725 3730 3735 Val Met
Leu Met Thr Val Ala Ser Gly Ser Trp 3740 3745 1313PRTBovine Viral
Diarrhea VirusMISC_FEATURE(1)..(13)Conserved protein sequence
within glycoprotein Erns 13Ser Leu His Gly Ile Trp Pro Glu Lys Ile
Cys Thr Gly 1 5 10 1420PRTBovine Viral Diarrhea
VirusMISC_FEATURE(1)..(20)Conserved protein sequence within
glycoprotein Erns 14Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys
Asn Trp Phe His 1 5 10 15 Ile Glu Pro Trp 20 1511PRTBovine Viral
Diarrhea VirusMISC_FEATURE(1)..(11)Conserved protein sequence
within glycoprotein Erns 15Ser Leu His Gly Ile Trp Pro Glu Lys Ile
Cys 1 5 10 1612PRTBovine Viral Diarrhea
VirusMISC_FEATURE(1)..(12)Conserved protein sequence within
glycoprotein Erns 16Arg His Glu Trp Asn Lys His Gly Trp Cys Asn Trp
1 5 10
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