U.S. patent application number 13/535775 was filed with the patent office on 2013-01-10 for delta 17 desaturase and its use in making polyunsaturated fatty acids. This patent application is currently assigned to E I DU PONT DE NEMOURS AND COMPANY. Invention is credited to Zhixiong Xue, Narendra S. Yadav, Quinn Qun Zhu.
Application Number | 20130011846 13/535775 |
Document ID | / |
Family ID | 39167662 |
Filed Date | 2013-01-10 |
United States Patent Application | 20130011846 |
Kind Code | A1 |
Yadav; Narendra S. ; et al. | January 10, 2013 |
The present invention relates to .DELTA.17 desaturases, which have the ability to convert .omega.-6 fatty acids into their .omega.-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding .DELTA.17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these .DELTA.17 desaturases in oleaginous yeast are disclosed.
Inventors: | Yadav; Narendra S.; (Wilmington, DE) ; Xue; Zhixiong; (Chadds Ford, PA) ; Zhu; Quinn Qun; (West Chester, PA) |
Assignee: | E I DU PONT DE NEMOURS AND
COMPANY Wilmington DE |
Family ID: | 39167662 |
Appl. No.: | 13/535775 |
Filed: | June 28, 2012 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
12469208 | May 20, 2009 | 8241884 | ||
13535775 | ||||
11779915 | Jul 19, 2007 | 7556949 | ||
12469208 | ||||
60855177 | Oct 30, 2006 | |||
Current U.S. Class: | 435/6.12 ; 435/189; 506/2; 536/23.2 |
Current CPC Class: | C12P 7/6472 20130101; C12P 7/6427 20130101; C12N 9/0083 20130101 |
Class at Publication: | 435/6.12 ; 536/23.2; 435/189; 506/2 |
International Class: | C12N 9/02 20060101 C12N009/02; C40B 20/00 20060101 C40B020/00; C12Q 1/68 20060101 C12Q001/68; C12N 15/53 20060101 C12N015/53 |
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 102 <210> SEQ ID NO 1 <211> LENGTH: 1080
<212> TYPE: DNA <213> ORGANISM: Pythium aphanidermatum
<400> SEQUENCE: 1 atggcttctt ccactgttgc tgcgccgtac gagttcccga
cgctgacgga gatcaagcgc 60 tcgctgccag cgcactgctt tgaggcctcg
gtcccgtggt cgctctacta caccgtgcgc 120 gcgctgggca tcgccggctc
gctcgcgctc ggcctctact acgcgcgcgc gctcgcgatc 180 gtgcaggagt
ttgccctgct ggatgcggtg ctctgcacgg ggtacattct gctgcagggc 240
atcgtattct gggggttctt caccatcggc catgactgcg gccacggcgc gttctcgcgt
300 tcgcacctgc tcaacttcag cgtcggcacg ctcattcact cgatcatcct
cacgccgtac 360 gagtcatgga agatctcgca ccgccaccac cacaagaaca
cgggcaacat cgacaaggac 420 gagattttct acccgcagcg cgaggccgac
tcgcacccac tgtcccgaca catggtgatc 480 tcgctcggct cggcctggtt
cgcgtacctc gttgcgggct tccctcctcg caaggtgaac 540 cacttcaacc
cttgggaacc gttgtacctg cgccgcatgt ctgccgtcat catctcactc 600
ggctcgctcg tggcgttcgc gggcttgtat gcgtatctca cctacgtcta tggccttaag
660 accatggcgc tgtactactt cgcccctctc tttgggttcg ccacgatgct
cgtggtcact 720 acctttttgc accacaatga cgaggaaacg ccatggtacg
ccgactcgga gtggacgtac 780 gtcaagggca acctctcgtc cgtggaccgc
tcgtacggcg cgctcatcga caacctgagc 840 cacaacatcg gcacgcacca
gatccaccac ctgtttccga tcatcccgca ctacaagctg 900 aacgaggcga
cggcagcgtt cgcgcaggcg ttcccggagc tcgtgcgcaa gagcgcgtcg 960
ccgatcatcc cgacgttcat ccgcatcggg ctcatgtacg ccaagtacgg cgtcgtggac
1020 aaggacgcca agatgtttac gctcaaggag gccaaggccg ccaagaccaa
ggccaactag 1080 <210> SEQ ID NO 2 <211> LENGTH: 359
<212> TYPE: PRT <213> ORGANISM: Pythium aphanidermatum
<220> FEATURE: <221> NAME/KEY: MISC_FEATURE <223>
OTHER INFORMATION: delta-17 desaturase <400> SEQUENCE: 2 Met
Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 1 5 10
15 Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro
20 25 30 Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly
Ser Leu 35 40 45 Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile
Val Gln Glu Phe 50 55 60 Ala Leu Leu Asp Ala Val Leu Cys Thr Gly
Tyr Ile Leu Leu Gln Gly 65 70 75 80 Ile Val Phe Trp Gly Phe Phe Thr
Ile Gly His Asp Cys Gly His Gly 85 90 95 Ala Phe Ser Arg Ser His
Leu Leu Asn Phe Ser Val Gly Thr Leu Ile 100 105 110 His Ser Ile Ile
Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 115 120 125 His His
His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 130 135 140
Pro Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 145
150 155 160 Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe
Pro Pro 165 170 175 Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu
Tyr Leu Arg Arg 180 185 190 Met Ser Ala Val Ile Ile Ser Leu Gly Ser
Leu Val Ala Phe Ala Gly 195 200 205 Leu Tyr Ala Tyr Leu Thr Tyr Val
Tyr Gly Leu Lys Thr Met Ala Leu 210 215 220 Tyr Tyr Phe Ala Pro Leu
Phe Gly Phe Ala Thr Met Leu Val Val Thr 225 230 235 240 Thr Phe Leu
His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 245 250 255 Glu
Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 260 265
270 Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile
275 280 285 His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu
Ala Thr 290 295 300 Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg
Lys Ser Ala Ser 305 310 315 320 Pro Ile Ile Pro Thr Phe Ile Arg Ile
Gly Leu Met Tyr Ala Lys Tyr 325 330 335 Gly Val Val Asp Lys Asp Ala
Lys Met Phe Thr Leu Lys Glu Ala Lys 340 345 350 Ala Ala Lys Thr Lys
Ala Asn 355 <210> SEQ ID NO 3 <211> LENGTH: 359
<212> TYPE: PRT <213> ORGANISM: Pythium aphanidermatum
<220> FEATURE: <221> NAME/KEY: MISC_FEATURE <223>
OTHER INFORMATION: delta-17 desaturase <220> FEATURE:
<221> NAME/KEY: MISC_FEATURE <222> LOCATION:
(155)..(155) <223> OTHER INFORMATION: Xaa = Ser or Pro
<220> FEATURE: <221> NAME/KEY: MISC_FEATURE <222>
LOCATION: (351)..(351) <223> OTHER INFORMATION: Xaa = Ala or
Thr <400> SEQUENCE: 3 Met Ala Ser Ser Thr Val Ala Ala Pro Tyr
Glu Phe Pro Thr Leu Thr 1 5 10 15 Glu Ile Lys Arg Ser Leu Pro Ala
His Cys Phe Glu Ala Ser Val Pro 20 25 30 Trp Ser Leu Tyr Tyr Thr
Val Arg Ala Leu Gly Ile Ala Gly Ser Leu 35 40 45 Ala Leu Gly Leu
Tyr Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 50 55 60 Ala Leu
Leu Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 65 70 75 80
Ile Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 85
90 95 Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu
Ile 100 105 110 His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile
Ser His Arg 115 120 125 His His His Lys Asn Thr Gly Asn Ile Asp Lys
Asp Glu Ile Phe Tyr 130 135 140 Pro Gln Arg Glu Ala Asp Ser His Pro
Leu Xaa Arg His Met Val Ile 145 150 155 160 Ser Leu Gly Ser Ala Trp
Phe Ala Tyr Leu Val Ala Gly Phe Pro Pro 165 170 175 Arg Lys Val Asn
His Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 180 185 190 Met Ser
Ala Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 195 200 205
Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 210
215 220 Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val
Thr 225 230 235 240 Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp
Tyr Ala Asp Ser 245 250 255 Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser
Ser Val Asp Arg Ser Tyr 260 265 270 Gly Ala Leu Ile Asp Asn Leu Ser
His Asn Ile Gly Thr His Gln Ile 275 280 285 His His Leu Phe Pro Ile
Ile Pro His Tyr Lys Leu Asn Glu Ala Thr 290 295 300 Ala Ala Phe Ala
Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 305 310 315 320 Pro
Ile Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 325 330
335 Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Xaa Lys
340 345 350 Ala Ala Lys Thr Lys Ala Asn 355 <210> SEQ ID NO 4
<211> LENGTH: 1080 <212> TYPE: DNA <213>
ORGANISM: Pythium aphanidermatum <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: syntthetic
delta-17 desaturase, codon-optimized for Yarrowia lipolytica
<400> SEQUENCE: 4 atggcttcct ctaccgttgc cgctccctac gagttcccta
ctctcaccga gatcaagcga 60 tccctgcctg cccactgctt cgaagcctct
gttccctggt ccctctacta taccgtgcga 120 gctctgggca ttgccggttc
ccttgctctc ggactgtact atgctcgagc ccttgctatc 180 gtgcaggagt
ttgcactgct cgatgccgtc ctttgcactg gctacattct gctccagggt 240
atcgtgttct ggggattctt taccatcggt cacgactgtg gacatggtgc cttctcgcga
300 tcccacctgc tcaacttctc tgttggcaca ctcattcact ccatcattct
gactccctac 360 gagtcgtgga agatcagcca tcgacaccat cacaagaaca
ccggcaacat cgacaaggat 420 gagatcttct accctcagcg agaagccgac
tctcatcccc tgtcccgaca catggtcatc 480 tcccttggtt cggcttggtt
tgcctacctc gttgctggat ttcctccccg aaaggtcaac 540 cacttcaatc
cctgggagcc tctctacctg cgaagaatgt ctgccgtcat catttccctc 600
ggctctctcg tggcctttgc tggtctgtac gcctacctta cctacgtcta cggcctcaag
660 accatggctc tgtattactt cgcacctctc tttggattcg ccaccatgct
ggttgtcact 720 accttcctcc atcacaacga cgaggaaact ccctggtacg
ccgattcgga gtggacctat 780 gtcaagggca acttgtcctc tgtggaccga
agctacggag ccctcatcga caacctgtcc 840 cacaacattg gtacacatca
gatccaccat ctgtttccca tcattcctca ctacaagctc 900 aacgaggcca
ctgctgcctt cgctcaggcc tttcccgaac tggtgcgaaa gtcggcttct 960
cccatcattc ccaccttcat ccgaattggt cttatgtacg ccaagtacgg cgtggtcgac
1020 aaggatgcca agatgtttac cctcaaggag gccaaggctg ccaagaccaa
agccaactaa 1080 <210> SEQ ID NO 5 <211> LENGTH: 614
<212> TYPE: DNA <213> ORGANISM: Pythium aphanidermatum
<400> SEQUENCE: 5 ttttgggggt tcttcaccgt cggccatgac tgcggccacg
gcgcgttctc gcgttcgcac 60 ctgctcaact tcagcgtcgg cacgctcatt
cactcgatca tcctcacgcc gtacgagtca 120 tggaagatct cgcaccgcca
ccaccacaag aacacgggca acatcgacaa ggacgagatt 180 ttctacccgc
agcgcgaggc cgactcgcac ccactgtccc gacacatggt gatctcgctc 240
ggctcggcct ggttcgcgta cctcgttgcg ggcttccctc ctcgcatggt gaaccacttc
300 aacccttggg aaccgttgta cctgcgccgc atgtctgccg tcatcatctc
actcggctcg 360 ctcgtggcgt tcgcgggctt gtatgcgtat ctcacctacg
tctatggcct taagaccatg 420 gcgctgtact acttcgcccc tctctttggg
ttcgccacga tgctcgtggt cactaccttt 480 ttgcaccaca atggcgagga
aacgccatgg tacgccgact cggagtggac gtacgtcaag 540 ggcaacctct
cgtccgtgga ccgctcgtac ggcgcgctca tcgacaacct gagccacaac 600
atcggcacgc acaa 614 <210> SEQ ID NO 6 <211> LENGTH: 739
<212> TYPE: DNA <213> ORGANISM: Pythium aphanidermatum
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (132)..(132) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 6 actatagggc acgcgtggtc gacggcccgg
gctggtatca aatacttttt ctaatttaat 60 atctacgaaa cgtttttttg
ctatgattgg cacctattca atgctcatga atctgatgat 120 agtatttgca
tnacttcatc ctctcttcca ttttatgctg actcaaacct ctttcgcgct 180
cggtttcaaa gggttacact actcgtgcgt ggtaccgagt gtaaccagca gcaaaaccgc
240 tccatacaac cgccaagtgt gaatgagggg cagacactgc gcgtgatctt
gttctatgcg 300 cagccagcca gtggaggtct ctcccgggcg tggacctcac
ttcagcttga gccgcggacc 360 gcgcagacca cccgacccgc acccgccatg
gcttcttcca ctgttgctgc gccgtacgag 420 ttcccgacgc tgacggagat
caagcgctcg ctgccagcgc actgctttga ggcctcggtc 480 ccgtggtcgc
tctactacac cgtgcgcgcg ctgggcatcg ccggctcgct cgcgctcggc 540
ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg ccctgctgga tgcggtgctc
600 tgcacggggt acattctgct gcagggcatc gtattctggg ggttcttcac
catcggccat 660 gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca
acttcagcgt cggcacgctc 720 attcactcga tcatcctca 739 <210> SEQ
ID NO 7 <211> LENGTH: 512 <212> TYPE: DNA <213>
ORGANISM: Pythium aphanidermatum <400> SEQUENCE: 7 ctgtactact
tcgcccctct ctttgggttc gccacgatgc tcgtggtcac tacctttttg 60
caccacaatg acgaggaaac gccatggtac gccgactcgg agtggacgta cgtcaagggc
120 aacctctcgt ccgtggaccg ctcgtacggc gcgctcattg acaacctgag
ccacaacatc 180 ggcacgcacc agatccacca cctgtttccg atcatcccgc
actacaagct gaacgaggcg 240 acggcagcgt tcgcgcaggc gttcccggag
ctcgtgcgca agagcgcgtc gccgatcatc 300 ccgacgttca tccgcatcgg
gctcatgtac gccaagtacg gcgtcgtgga caaggacgcc 360 aagatgttta
cgctcaagga ggccaaggcc gccaagacca aggccaacta ggcagaggca 420
aacaaggaag agaagttgtg tataggctcg taatgaacat gcgggttttt tgtttttwww
480 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 512 <210> SEQ ID NO 8
<211> LENGTH: 1533 <212> TYPE: DNA <213>
ORGANISM: Pythium aphanidermatum <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: delta-17
desaturase contig (CDS corresponds to nucleotides 388-1467)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (132)..(132) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 8 actatagggc acgcgtggtc gacggcccgg
gctggtatca aatacttttt ctaatttaat 60 atctacgaaa cgtttttttg
ctatgattgg cacctattca atgctcatga atctgatgat 120 agtatttgca
tnacttcatc ctctcttcca ttttatgctg actcaaacct ctttcgcgct 180
cggtttcaaa gggttacact actcgtgcgt ggtaccgagt gtaaccagca gcaaaaccgc
240 tccatacaac cgccaagtgt gaatgagggg cagacactgc gcgtgatctt
gttctatgcg 300 cagccagcca gtggaggtct ctcccgggcg tggacctcac
ttcagcttga gccgcggacc 360 gcgcagacca cccgacccgc acccgccatg
gcttcttcca ctgttgctgc gccgtacgag 420 ttcccgacgc tgacggagat
caagcgctcg ctgccagcgc actgctttga ggcctcggtc 480 ccgtggtcgc
tctactacac cgtgcgcgcg ctgggcatcg ccggctcgct cgcgctcggc 540
ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg ccctgctgga tgcggtgctc
600 tgcacggggt acattctgct gcagggcatc gtattctggg ggttcttcac
catcggccat 660 gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca
acttcagcgt cggcacgctc 720 attcactcga tcatcctcac gccgtacgag
tcatggaaga tctcgcaccg ccaccaccac 780 aagaacacgg gcaacatcga
caaggacgag attttctacc cgcagcgcga ggccgactcg 840 cacccactgt
cccgacacat ggtgatctcg ctcggctcgg cctggttcgc gtacctcgtt 900
gcgggcttcc ctcctcgcat ggtgaaccac ttcaaccctt gggaaccgtt gtacctgcgc
960 cgcatgtctg ccgtcatcat ctcactcggc tcgctcgtgg cgttcgcggg
cttgtatgcg 1020 tatctcacct acgtctatgg ccttaagacc atggcgctgt
actacttcgc ccctctcttt 1080 gggttcgcca cgatgctcgt ggtcactacc
tttttgcacc acaatgrcga ggaaacgcca 1140 tggtacgccg actcggagtg
gacgtacgtc aagggcaacc tctcgtccgt ggaccgctcg 1200 tacggcgcgc
tcattgacaa cctgagccac aacatcggca cgcaccagat ccaccacctg 1260
tttccgatca tcccgcacta caagctgaac gaggcgacgg cagcgttcgc gcaggcgttc
1320 ccggagctcg tgcgcaagag cgcgtcgccg atcatcccga cgttcatccg
catcgggctc 1380 atgtacgcca agtacggcgt cgtggacaag gacgccaaga
tgtttacgct caaggaggcc 1440 aaggccgcca agaccaaggc caactaggca
gaggcaaaca aggaagagaa gttgtgtata 1500 ggctcgtaat gaacatgcgg
gttttttgtt ttt 1533 <210> SEQ ID NO 9 <211> LENGTH: 39
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: SMART IV
oligonucleotide <400> SEQUENCE: 9 aagcagtggt atcaacgcag
agtggccatt acggccggg 39 <210> SEQ ID NO 10 <211>
LENGTH: 59 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
CDSIII/3'PCR primer <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (28)..(57) <223> OTHER
INFORMATION: thymidine (dT); see BD Biosciences Clontech's SMART
cDNA technology <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (59)..(59) <223> OTHER
INFORMATION: n is a, c, g, or t <400> SEQUENCE: 10 attctagagg
ccgaggcggc cgacatgttt tttttttttt tttttttttt tttttttvn 59
<210> SEQ ID NO 11 <211> LENGTH: 23 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5'-PCR primer <400> SEQUENCE:
11 aagcagtggt atcaacgcag agt 23 <210> SEQ ID NO 12
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F1 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (18)..(18)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 12 ttytggggnt tyttyacngt 20 <210> SEQ ID NO 13
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primer PD17-F1 <400> SEQUENCE: 13
Phe Trp Gly Phe Phe Thr Tyr 1 5 <210> SEQ ID NO 14
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F2 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(15)..(15) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 14 ttcttyacng tnggncayga 20 <210> SEQ
ID NO 15 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-F3 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(15)..(15) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 15 tttttyacng tnggncayga 20 <210> SEQ
ID NO 16 <211> LENGTH: 7 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Translation of primers PD17-F2 and PD17-F3
<400> SEQUENCE: 16 Phe Phe Thr Val Gly His Asp 1 5
<210> SEQ ID NO 17 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-F4 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (3)..(3)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(9)..(9) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 17 acncaycgnc aycaycayaa 20 <210> SEQ
ID NO 18 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-F5 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (3)..(3) <223>
OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 18
acncayagrc aycaycayaa 20 <210> SEQ ID NO 19 <211>
LENGTH: 7 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Translation of primers PD17-F4 and PD17-F5 <400> SEQUENCE: 19
Thr His Arg His His His Lys 1 5 <210> SEQ ID NO 20
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F6 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 20 aaraayacng gnaayatyga 20 <210> SEQ ID NO 21
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F7 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 21 aaraayacng gnaayataga 20 <210> SEQ ID NO 22
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primers PD17-F6 and PD17-F7 <400>
SEQUENCE: 22 Lys Asn Thr Gly Asn Ile Asp 1 5 <210> SEQ ID NO
23 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-R1 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (15)..(15) <223>
OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 23
tcrtcrttrt grtgnagraa 20 <210> SEQ ID NO 24 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
PD17-R2 <400> SEQUENCE: 24 tcrtcrttrt grtgyaaraa 20
<210> SEQ ID NO 25 <211> LENGTH: 7 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Translation of primers PD17-R1 and
PD17-R2 <400> SEQUENCE: 25 Phe Leu His His Asn Asp Glu 1 5
<210> SEQ ID NO 26 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-R3 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (18)..(18)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 26 aaraargcyt tdatdatngg 20 <210> SEQ ID NO 27
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-R4 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (18)..(18) <223>
OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 27
aaraaygcyt tdatdatngg 20 <210> SEQ ID NO 28 <211>
LENGTH: 7 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Translation of primers PD17-R3 and PD17-R4 <400> SEQUENCE: 28
Pro Ile Ile Lys Ala Phe Phe 1 5 <210> SEQ ID NO 29
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-R5 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (9)..(9)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 29 ttrtgngtnc cdatrttatg 20 <210> SEQ ID NO 30
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-R6 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (9)..(9)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 30 ttrtgngtnc cdatrttgtg 20 <210> SEQ ID NO 31
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primers PD17-R5 and PD17-R6 <400>
SEQUENCE: 31 His Asn Ile Gly Thr His Gln 1 5 <210> SEQ ID NO
32 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-R7 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 32 ccyttnacrt angtccaytc 20 <210> SEQ ID NO 33
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primer PD17-R7 <400> SEQUENCE: 33
Glu Trp Thr Tyr Val Lys Gly 1 5 <210> SEQ ID NO 34
<211> LENGTH: 44 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Genome Walker adaptor-1 <400> SEQUENCE: 34
gtaatacgac tatagggcac gcgtggtcga cggcccgggc tggt 44 <210> SEQ
ID NO 35 <211> LENGTH: 8 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Genome Walker adaptor-2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(1)
<223> OTHER INFORMATION: 5' end is associated with a -PO4
group <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (8)..(8) <223> OTHER INFORMATION: 3'
end is associated with a -H2N group <400> SEQUENCE: 35
accagccc 8 <210> SEQ ID NO 36 <211> LENGTH: 22
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer
PUD17-5-1 <400> SEQUENCE: 36 aatctcgtcc ttgtcgatgt tg 22
<210> SEQ ID NO 37 <211> LENGTH: 22 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AP1 <400> SEQUENCE: 37
gtaatacgac tcactatagg gc 22 <210> SEQ ID NO 38 <211>
LENGTH: 22 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
PUD17-5-3 <400> SEQUENCE: 38 tgaggatgat cgagtgaatg ag 22
<210> SEQ ID NO 39 <211> LENGTH: 19 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AP2 <400> SEQUENCE: 39
actatagggc acgcgtggt 19 <210> SEQ ID NO 40 <211>
LENGTH: 22 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
PUD17-3-1 <400> SEQUENCE: 40 cacctacgtc tatggcctta ag 22
<210> SEQ ID NO 41 <211> LENGTH: 22 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PUD17-3-2 <400>
SEQUENCE: 41 ctgtactact tcgcccctct ct 22 <210> SEQ ID NO 42
<211> LENGTH: 1085 <212> TYPE: DNA <213>
ORGANISM: Phytophthora infestans <400> SEQUENCE: 42
atggcgacga aggaggcgta tgtgttcccc actctgacgg agatcaagcg gtcgctacct
60 aagactgttt cgaggcttcg gtgcctctgt cgctctacta caccgtgcgt
tgtctggtga 120 tcgcggtggc tctaaccttc ggtctcaact acgctcgcgc
tctgcccgag gtcgagagct 180 tctgggctct ggacgccgca ctctgcacgg
gctacatctt gctgcagggc atcgtgttct 240 ggggcttctt cacggtgggc
cacgatgccg gccacggcgc cttctcgcgc taccacctgc 300 ttaacttcgt
ggtgggcact ttcatgcact cgctcatcct cacgcccttc gagtcgtgga 360
agctcacgca ccgtcaccac cacaagaaca cgggcaacat tgaccgtgac gaggtcttct
420 acccgcaacg caaggccgac gaccacccgc tgtctcgcaa cctgattctg
gcgctcgggg 480 cagcgtggct cgcctatttg gtcgagggct tccctcctcg
taaggtcaac cacttcaacc 540 cgttcgagcc tctgttcgtg cgtcaggtgt
cagctgtggt aatctctctt ctcgcccact 600 tcttcgtggc cggactctcc
atctatctga gcctccagct gggccttaag acgatggcaa 660 tctactacta
tggacctgtt tttgtgttcg gcagcatgct ggtcattacc accttcctac 720
accacaatga tgaggagacc ccatggtacg ccgactcgga gtggacgtac gtcaagggca
780 acctctcgtc cgtggaccga tcgtacggcg cgctcattga caacctgagc
cacaacatcg 840 gcacgcacca gatccaccac cttttcccta tcattccgca
ctacaaactc aagaaagcca 900 ctgcggcctt ccaccaggct ttccctgagc
tcgtgcgcaa gagcgacgag ccaattatca 960 aggctttctt ccgggttgga
cgtctctacg caaactacgg cgttgtggac caggaggcga 1020 agctcttcac
gctaaaggaa gccaaggcgg cgaccgaggc ggcggccaag accaagtcca 1080 cgtaa
1085 <210> SEQ ID NO 43 <211> LENGTH: 361 <212>
TYPE: PRT <213> ORGANISM: Phytophthora infestans <300>
PUBLICATION INFORMATION: <302> TITLE: METHOD FOR PRODUCING
UNSATURATED Omega3 FATTY ACIDS IN TRANSGENIC ORGANISMS <308>
DATABASE ACCESSION NUMBER: CAJ30870 <309> DATABASE ENTRY
DATE: 2005-09-21 <310> PATENT DOCUMENT NUMBER: WO 2005083053
<311> PATENT FILING DATE: 2005-02-23 <312> PUBLICATION
DATE: 2005-09-09 <313> RELEVANT RESIDUES IN SEQ ID NO:
(1)..(361) <400> SEQUENCE: 43 Met Ala Thr Lys Glu Ala Tyr Val
Phe Pro Thr Leu Thr Glu Ile Lys 1 5 10 15 Arg Ser Leu Pro Lys Asp
Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 20 25 30 Tyr Tyr Thr Val
Arg Cys Leu Val Ile Ala Val Ala Leu Thr Phe Gly 35 40 45 Leu Asn
Tyr Ala Arg Ala Leu Pro Glu Val Glu Ser Phe Trp Ala Leu 50 55 60
Asp Ala Ala Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly Ile Val Phe 65
70 75 80 Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala
Phe Ser 85 90 95 Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe
Met His Ser Leu 100 105 110 Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu
Thr His Arg His His His 115 120 125 Lys Asn Thr Gly Asn Ile Asp Arg
Asp Glu Val Phe Tyr Pro Gln Arg 130 135 140 Lys Ala Asp Asp His Pro
Leu Ser Arg Asn Leu Ile Leu Ala Leu Gly 145 150 155 160 Ala Ala Trp
Leu Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 165 170 175 Asn
His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ser Ala 180 185
190 Val Val Ile Ser Leu Leu Ala His Phe Phe Val Ala Gly Leu Ser Ile
195 200 205 Tyr Leu Ser Leu Gln Leu Gly Leu Lys Thr Met Ala Ile Tyr
Tyr Tyr 210 215 220 Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile
Thr Thr Phe Leu 225 230 235 240 His His Asn Asp Glu Glu Thr Pro Trp
Tyr Ala Asp Ser Glu Trp Thr 245 250 255 Tyr Val Lys Gly Asn Leu Ser
Ser Val Asp Arg Ser Tyr Gly Ala Leu 260 265 270 Ile Asp Asn Leu Ser
His Asn Ile Gly Thr His Gln Ile His His Leu 275 280 285 Phe Pro Ile
Ile Pro His Tyr Lys Leu Lys Lys Ala Thr Ala Ala Phe 290 295 300 His
Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Ile 305 310
315 320 Lys Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val
Val 325 330 335 Asp Gln Glu Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys
Ala Ala Thr 340 345 350 Glu Ala Ala Ala Lys Thr Lys Ser Thr 355 360
<210> SEQ ID NO 44 <211> LENGTH: 1092 <212> TYPE:
DNA <213> ORGANISM: Phytophthora sojae <400> SEQUENCE:
44 atggcgtcca agcaggagca gccgtaccag ttcccgacgc tgacggagat
caagcgctcg 60 ctgcccagcg agtgtttcga ggcgtccgtg ccgctctcgc
tctactacac ggtgcgctgc 120 ctggtgatcg ccgtgtcgct ggccttcggg
ctccaccacg cgcgctcgct gcccgtggtc 180 gagggcctct gggcgctgga
cgccgcgctc tgcacgggct acgtgctgct gcagggcatc 240 gtgttctggg
gcttcttcac cgtgggccat gacgccggcc acggcgcctt ctcgcgctac 300
cacctgctca acttcgtgat cggcaccttc atccactcgc tcatcctgac gcccttcgag
360 tcgtggaagc tcacgcaccg ccaccaccac aagaacacgg gcaacatcga
ccgcgacgag 420 atcttctacc cgcagcgcaa ggccgacgac cacccgctct
cgcgtaacct catcctggcg 480 ctgggcgccg cgtggttcgc ctacctggtc
gagggcttcc cgccgcgcaa ggtcaaccac 540 ttcaacccgt tcgagccgct
gttcgtccgc caggtgtccg ccgtggtcat ctcgctggcc 600 gcgcacttcg
gcgtggccgc gctgtccatc tacctgagcc tgcagttcgg cttcaagacc 660
atggctatct actactacgg gcccgtgttc gtgttcggca gcatgctggt catcaccacc
720 ttcctgcacc acaacgacga ggagaccccc tggtacgccg actcggagtg
gacctacgtc 780 aagggcaacc tctcgtcggt cgaccgctcc tacggcgcgc
tcatcgacaa cctgagccac 840 aacatcggca cgcaccagat ccaccacctc
ttccccatca tcccgcacta taagctcaag 900 cgcgccaccg aggccttcca
ccaggcgttc cccgagctcg tgcgcaagag cgacgagccc 960 atcattaagg
ccttcttccg cgtcggccgc ctctacgcca actacggcgt cgtggactcg 1020
gacgccaagc tcttcacgct caaggaggcc aaggccgtgt ccgaggcggc gaccaagact
1080 aaggccaact ga 1092 <210> SEQ ID NO 45 <211>
LENGTH: 363 <212> TYPE: PRT <213> ORGANISM:
Phytophthora sojae <400> SEQUENCE: 45 Met Ala Ser Lys Gln Glu
Gln Pro Tyr Gln Phe Pro Thr Leu Thr Glu 1 5 10 15 Ile Lys Arg Ser
Leu Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu 20 25 30 Ser Leu
Tyr Tyr Thr Val Arg Cys Leu Val Ile Ala Val Ser Leu Ala 35 40 45
Phe Gly Leu His His Ala Arg Ser Leu Pro Val Val Glu Gly Leu Trp 50
55 60 Ala Leu Asp Ala Ala Leu Cys Thr Gly Tyr Val Leu Leu Gln Gly
Ile 65 70 75 80 Val Phe Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly
His Gly Ala 85 90 95 Phe Ser Arg Tyr His Leu Leu Asn Phe Val Ile
Gly Thr Phe Ile His 100 105 110 Ser Leu Ile Leu Thr Pro Phe Glu Ser
Trp Lys Leu Thr His Arg His 115 120 125 His His Lys Asn Thr Gly Asn
Ile Asp Arg Asp Glu Ile Phe Tyr Pro 130 135 140 Gln Arg Lys Ala Asp
Asp His Pro Leu Ser Arg Asn Leu Ile Leu Ala 145 150 155 160 Leu Gly
Ala Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg 165 170 175
Lys Val Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val 180
185 190 Ser Ala Val Val Ile Ser Leu Ala Ala His Phe Gly Val Ala Ala
Leu 195 200 205 Ser Ile Tyr Leu Ser Leu Gln Phe Gly Phe Lys Thr Met
Ala Ile Tyr 210 215 220 Tyr Tyr Gly Pro Val Phe Val Phe Gly Ser Met
Leu Val Ile Thr Thr 225 230 235 240 Phe Leu His His Asn Asp Glu Glu
Thr Pro Trp Tyr Ala Asp Ser Glu 245 250 255 Trp Thr Tyr Val Lys Gly
Asn Leu Ser Ser Val Asp Arg Ser Tyr Gly 260 265 270 Ala Leu Ile Asp
Asn Leu Ser His Asn Ile Gly Thr His Gln Ile His 275 280 285 His Leu
Phe Pro Ile Ile Pro His Tyr Lys Leu Lys Arg Ala Thr Glu 290 295 300
Ala Phe His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro 305
310 315 320 Ile Ile Lys Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn
Tyr Gly 325 330 335 Val Val Asp Ser Asp Ala Lys Leu Phe Thr Leu Lys
Glu Ala Lys Ala 340 345 350 Val Ser Glu Ala Ala Thr Lys Thr Lys Ala
Asn 355 360 <210> SEQ ID NO 46 <211> LENGTH: 1086
<212> TYPE: DNA <213> ORGANISM: Phytophthora ramorum
<400> SEQUENCE: 46 atggcgacta agcagccgta ccagttcccg
accctgacgg agatcaagcg gtcgctgccc 60 agcgagtgct ttgaggcctc
ggtgccgctg tcgctctact acacggtgcg catcgtggcc 120 atcgccgtgg
cgctggcgtt cggcctcaac tacgcgcgcg cgctgcccgt ggtcgagagc 180
ttgtgggcgc tggacgctgc gctctgctgc ggttacgtgc tgctgcaggg catcgtgttc
240 tggggcttct tcacggtggg ccatgacgcc ggccacggcg ccttctcgcg
ttaccacctg 300 ctcaacttcg tggtgggcac cttcatccac tcgctcatcc
tcacgccctt cgagtcgtgg 360 aagctcacgc accgccacca ccacaagaac
acgggcaaca ttgaccgcga cgagatcttc 420 tacccgcagc gcaaggccga
cgaccacccg ctgtcgcgca acctcgtgct ggcgctcggc 480 gccgcgtggt
tcgcctacct ggtcgagggc ttcccgcccc gcaaggtcaa ccacttcaac 540
ccattcgagc cgctgtttgt gcgccaggtg gccgccgtcg tcatctcgct ctccgcgcac
600 ttcgccgtgt tggcgctgtc cgtgtatctg agcttccagt tcggtctcaa
gaccatggcg 660 ctctactact acggccccgt cttcgtgttc ggcagcatgc
ttgtgatcac caccttcctg 720 catcacaatg acgaggagac cccatggtac
ggagactccg actggaccta cgtcaagggc 780 aacctgtcgt ccgtggaccg
gtcctacggc gcgttcatcg acaacctgag ccacaacatc 840 ggcacgcacc
agatccacca cctcttcccc atcatcccgc actacaagct caaccgcgct 900
acggcggcat tccaccaggc cttccccgag ctcgtgcgca agagcgacga gccgatcctc
960 aaggccttct ggcgcgtcgg ccgactgtac gccaactacg gcgtcgtgga
cccggacgcc 1020 aagctcttca cgctcaagga ggccaaggcg gcgtccgagg
cggcgaccaa gaccaaggcc 1080 acctaa 1086 <210> SEQ ID NO 47
<211> LENGTH: 361 <212> TYPE: PRT <213> ORGANISM:
Phytophthora ramorum <400> SEQUENCE: 47 Met Ala Thr Lys Gln
Pro Tyr Gln Phe Pro Thr Leu Thr Glu Ile Lys 1 5 10 15 Arg Ser Leu
Pro Ser Glu Cys Phe Glu Ala Ser Val Pro Leu Ser Leu 20 25 30 Tyr
Tyr Thr Val Arg Ile Val Ala Ile Ala Val Ala Leu Ala Phe Gly 35 40
45 Leu Asn Tyr Ala Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu
50 55 60 Asp Ala Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile
Val Phe 65 70 75 80 Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His
Gly Ala Phe Ser 85 90 95 Arg Tyr His Leu Leu Asn Phe Val Val Gly
Thr Phe Ile His Ser Leu 100 105 110 Ile Leu Thr Pro Phe Glu Ser Trp
Lys Leu Thr His Arg His His His 115 120 125 Lys Asn Thr Gly Asn Ile
Asp Arg Asp Glu Ile Phe Tyr Pro Gln Arg 130 135 140 Lys Ala Asp Asp
His Pro Leu Ser Arg Asn Leu Val Leu Ala Leu Gly 145 150 155 160 Ala
Ala Trp Phe Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg Lys Val 165 170
175 Asn His Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala
180 185 190 Val Val Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu
Ser Val 195 200 205 Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala
Leu Tyr Tyr Tyr 210 215 220 Gly Pro Val Phe Val Phe Gly Ser Met Leu
Val Ile Thr Thr Phe Leu 225 230 235 240 His His Asn Asp Glu Glu Thr
Pro Trp Tyr Gly Asp Ser Asp Trp Thr 245 250 255 Tyr Val Lys Gly Asn
Leu Ser Ser Val Asp Arg Ser Tyr Gly Ala Phe 260 265 270 Ile Asp Asn
Leu Ser His Asn Ile Gly Thr His Gln Ile His His Leu 275 280 285 Phe
Pro Ile Ile Pro His Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe 290 295
300 His Gln Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu
305 310 315 320 Lys Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr
Gly Val Val 325 330 335 Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu
Ala Lys Ala Ala Ser 340 345 350 Glu Ala Ala Thr Lys Thr Lys Ala Thr
355 360 <210> SEQ ID NO 48 <211> LENGTH: 7145
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid
pKUNFmkF2 <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (692)..(695) <223> OTHER INFORMATION: n
is a, c, g, or t <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (700)..(700) <223> OTHER
INFORMATION: n is a, c, g, or t <400> SEQUENCE: 48 catggcgtcc
acttcggctc tgcccaagca gaaccctgcg cttagacgca ccgtcacctc 60
aactactgtg acggattctg agtctgccgc cgtctctcct tcagactctc cccgccactc
120 ggcctcttcc acatcgctct cgtccatgtc cgaggttgat atcgccaagc
ccaagtccga 180 gtatggtgtc atgctcgaca cctacggcaa ccagttcgag
gttcccgact ttaccatcaa 240 ggacatctac aatgccatcc ctaagcactg
cttcaagcgc tccgctctca agggatacgg 300 ttatatcctc cgcgacattg
tcctcctgac taccactttc agcatctggt acaactttgt 360 gacccccgaa
tatatcccct ccacccccgc ccgcgctggt ctgtgggccg tgtacaccgt 420
tcttcagggt cttttcggta ctggtctctg ggttattgcc catgagtgcg gtcacggtgc
480 tttctccgat tctcgcatca tcaacgacat tactggctgg gttcttcact
cttccctcct 540 tgtcccctac ttcagctggc aaatctccca ccgaaagcac
cacaaggcca ctggcaacat 600 ggagcgtgac atggtcttcg ttccccgaac
ccgcgagcag caggctactc gtctcggaaa 660 gatgacccac gagctcgctc
atcttactga gnnnntcgtn ggctggccca actacctcat 720 caccaatgtt
accggccaca actaccacga gcgccagcgt gagggtcgcg gcaagggcaa 780
gcataacggc ctcggcggtg gtgttaacca cttcgatccc cgcagccctc tgtacgagaa
840 cagtgacgct aagctcatcg tcctcagcga tattggtatc ggtctgatgg
ccactgctct 900 gtacttcctc gttcagaagt tcggtttcta caacatggcc
atctggtact ttgttcccta 960 cctctgggtt aaccactggc tcgttgccat
caccttcctc cagcacaccg accctaccct 1020 tccccactac accaacgacg
agtggaactt cgtccgtggt gccgctgcta ccattgaccg 1080 tgagatgggc
ttcatcggcc gccaccttct ccacggcatc atcgagactc atgtcctcca 1140
ccactacgtc agcagcatcc ccttctacaa cgcggacgag gccaccgagg ccattaagcc
1200 catcatgggc aagcactacc gggctgatgt ccaggatggt cctcgtggct
tcatccgcgc 1260 catgtaccgc agtgcgcgta tgtgccagtg ggttgagccc
agcgctggtg ccgagggtgc 1320 tggtaagggt gttctgttct tccgcaaccg
caacaacgtg ggcacccccc ccgctgttat 1380 caagcccgtt gcttaagtag
gcgcggccgc tatttatcac tctttacaac ttctacctca 1440 actatctact
ttaataaatg aatatcgttt attctctatg attactgtat atgcgttcct 1500
ctaagacaaa tcgaaaccag catgtgatcg aatggcatac aaaagtttct tccgaagttg
1560 atcaatgtcc tgatagtcag gcagcttgag aagattgaca caggtggagg
ccgtagggaa 1620 ccgatcaacc tgtctaccag cgttacgaat ggcaaatgac
gggttcaaag ccttgaatcc 1680 ttgcaatggt gccttggata ctgatgtcac
aaacttaaga agcagccgct tgtcctcttc 1740 ctcgatcgat ggtcatagct
gtttcctgtg tgaaattgtt atccgctcac aattccacac 1800 aacgtacgaa
gtcgtcaatg atgtcgatat gggttttgat catgcacaca taaggtccga 1860
ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa gcacacaggt
1920 tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac
ttgtggacgt 1980 tagctcgagc ttcgtaggag ggcattttgg tggtgaagag
gagactgaaa taaatttagt 2040 ctgcagaact ttttatcgga accttatctg
gggcagtgaa gtatatgtta tggtaatagt 2100 tacgagttag ttgaacttat
agatagactg gactatacgg ctatcggtcc aaattagaaa 2160 gaacgtcaat
ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc 2220
cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac gcagctgtca
2280 gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac
tcatagttgg 2340 agtcgtactc caaaggcggc aatgacgagt cagacagata
ctcgtcgacc ttttccttgg 2400 gaaccaccac cgtcagccct tctgactcac
gtattgtagc caccgacaca ggcaacagtc 2460 cgtggatagc agaatatgtc
ttgtcggtcc atttctcacc aactttaggc gtcaagtgaa 2520 tgttgcagaa
gaagtatgtg ccttcattga gaatcggtgt tgctgatttc aataaagtct 2580
tgagatcagt ttggcgcgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
2640 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
cggtcgttcg 2700 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
cggttatcca cagaatcagg 2760 ggataacgca ggaaagaaca tgtgagcaaa
aggccagcaa aaggccagga accgtaaaaa 2820 ggccgcgttg ctggcgtttt
tccataggct ccgcccccct gacgagcatc acaaaaatcg 2880 acgctcaagt
cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 2940
tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
3000 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
atctcagttc 3060 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
ccccccgttc agcccgaccg 3120 ctgcgcctta tccggtaact atcgtcttga
gtccaacccg gtaagacacg acttatcgcc 3180 actggcagca gccactggta
acaggattag cagagcgagg tatgtaggcg gtgctacaga 3240 gttcttgaag
tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 3300
tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
3360 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
gaaaaaaagg 3420 atctcaagaa gatcctttga tcttttctac ggggtctgac
gctcagtgga acgaaaactc 3480 acgttaaggg attttggtca tgagattatc
aaaaaggatc ttcacctaga tccttttaaa 3540 ttaaaaatga agttttaaat
caatctaaag tatatatgag taaacttggt ctgacagtta 3600 ccaatgctta
atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 3660
tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
3720 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
caataaacca 3780 gccagccgga agggccgagc gcagaagtgg tcctgcaact
ttatccgcct ccatccagtc 3840 tattaattgt tgccgggaag ctagagtaag
tagttcgcca gttaatagtt tgcgcaacgt 3900 tgttgccatt gctacaggca
tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 3960 ctccggttcc
caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4020
tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
4080 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
gcttttctgt 4140 gactggtgag tactcaacca agtcattctg agaatagtgt
atgcggcgac cgagttgctc 4200 ttgcccggcg tcaatacggg ataataccgc
gccacatagc agaactttaa aagtgctcat 4260 cattggaaaa cgttcttcgg
ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 4320 ttcgatgtaa
cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4380
ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
4440 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
atcagggtta 4500 ttgtctcatg agcggataca tatttgaatg tatttagaaa
aataaacaaa taggggttcc 4560 gcgcacattt ccccgaaaag tgccacctga
tgcggtgtga aataccgcac agatgcgtaa 4620 ggagaaaata ccgcatcagg
aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 4680 tttttgttaa
atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 4740
atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
4800 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
gcgatggccc 4860 actacgtgaa ccatcaccct aatcaagttt tttggggtcg
aggtgccgta aagcactaaa 4920 tcggaaccct aaagggagcc cccgatttag
agcttgacgg ggaaagccgg cgaacgtggc 4980 gagaaaggaa gggaagaaag
cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 5040 cacgctgcgc
gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat 5100
tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
5160 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
gccagggttt 5220 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
aatacgactc actatagggc 5280 gaattgggcc cgacgtcgca tgcagtggtg
gtattgtgac tggggatgta gttgagaata 5340 agtcatacac aagtcagctt
tcttcgagcc tcatataagt ataagtagtt caacgtatta 5400 gcactgtacc
cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca 5460
tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc
5520 atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt
taaattacat 5580 atccatagtc taacctctaa cagttaatct tctggtaagc
ctcccagcca gccttctggt 5640 atcgcttggc ctcctcaata ggatctcggt
tctggccgta cagacctcgg ccgacaatta 5700 tgatatccgt tccggtagac
atgacatcct caacagttcg gtactgctgt ccgagagcgt 5760 ctcccttgtc
gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct 5820
taattaattt gaatcgaatc gatgagccta aaatgaaccc gagtatatct cataaaattc
5880 tcggtgagag gtctgtgact gtcagtacaa ggtgccttca ttatgccctc
aaccttacca 5940 tacctcactg aatgtagtgt acctctaaaa atgaaataca
gtgccaaaag ccaaggcact 6000 gagctcgtct aacggacttg atatacaacc
aattaaaaca aatgaaaaga aatacagttc 6060 tttgtatcat ttgtaacaat
taccctgtac aaactaaggt attgaaatcc cacaatattc 6120 ccaaagtcca
cccctttcca aattgtcatg cctacaactc atataccaag cactaaccta 6180
ccgtttaaac agtgtacgca gatctactat agaggaacat ttaaattgcc ccggagaaga
6240 cggccaggcc gcctagatga caaattcaac aactcacagc tgactttctg
ccattgccac 6300 tagggggggg cctttttata tggccaagcc aagctctcca
cgtcggttgg gctgcaccca 6360 acaataaatg ggtagggttg caccaacaaa
gggatgggat ggggggtaga agatacgagg 6420 ataacggggc tcaatggcac
aaataagaac gaatactgcc attaagactc gtgatccagc 6480 gactgacacc
attgcatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc 6540
tggacaccac agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc
6600 agaaaacgct ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc
gctgaggtcg 6660 agcagggtgg tgtgacttgt tatagccttt agagctgcga
aagcgcgtat ggatttggct 6720 catcaggcca gattgagggt ctgtggacac
atgtcatgtt agtgtacttc aatcgccccc 6780 tggatatagc cccgacaata
ggccgtggcc tcattttttt gccttccgca catttccatt 6840 gctcgatacc
cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga 6900
ccaacatctt acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc
6960 ggttgccagt ctcttttttc ctttctttcc ccacagattc gaaatctaaa
ctacacatca 7020 cagaattccg agccgtgagt atccacgaca agatcagtgt
cgagacgacg cgttttgtgt 7080 aatgacacaa tccgaaagtc gctagcaaca
cacactctct acacaaacta acccagctct 7140 ggtac 7145 <210> SEQ ID
NO 49 <211> LENGTH: 5473 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pDMW287F <400> SEQUENCE: 49
ggccgcattg atgattggaa acacacacat gggttatatc taggtgagag ttagttggac
60 agttatatat taaatcagct atgccaacgg taacttcatt catgtcaacg
aggaaccagt 120 gactgcaagt aatatagaat ttgaccacct tgccattctc
ttgcactcct ttactatatc 180 tcatttattt cttatataca aatcacttct
tcttcccagc atcgagctcg gaaacctcat 240 gagcaataac atcgtggatc
tcgtcaatag agggcttttt ggactccttg ctgttggcca 300 ccttgtcctt
gctgtctggc tcattctgtt tcaacgcctt ttaattaatc gagcttggcg 360
taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
420 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
ctaactcaca 480 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat 540 taatgaatcg gccaacgcgc ggggagaggc
ggtttgcgta ttgggcgctc ttccgcttcc 600 tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca 660 aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 720
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
780 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg 840 acaggactat aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt 900 ccgaccctgc cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt 960 tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 1020 tgtgtgcacg
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 1080
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1140 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc 1200 tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa 1260 agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt 1320 tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct 1380 acggggtctg
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1440
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1500 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc 1560 tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact 1620 acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc 1680 tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt 1740 ggtcctgcaa
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1800
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1860 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
aaggcgagtt 1920 acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc 1980 agaagtaagt tggccgcagt gttatcactc
atggttatgg cagcactgca taattctctt 2040 actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc 2100 tgagaatagt
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 2160
gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
2220 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac 2280 tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa 2340 aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt 2400 tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa 2460 tgtatttaga
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2520
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2580 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc 2640 acgttcgccg gctttccccg tcaagctcta aatcgggggc
tccctttagg gttccgattt 2700 agtgctttac ggcacctcga ccccaaaaaa
cttgattagg gtgatggttc acgtagtggg 2760 ccatcgccct gatagacggt
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2820 ggactcttgt
tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 2880
taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2940 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
aggctgcgca 3000 actgttggga agggcgatcg gtgcgggcct cttcgctatt
acgccagctg gcgaaagggg 3060 gatgtgctgc aaggcgatta agttgggtaa
cgccagggtt ttcccagtca cgacgttgta 3120 aaacgacggc cagtgaattg
taatacgact cactataggg cgaattgggt accgggcccc 3180 ccctcgaggt
cgacgtttaa acagtgtacg cagtactata gaggaacatc gattgccccg 3240
gagaagacgg ccaggccgcc tagatgacaa attcaacaac tcacagctga ctttctgcca
3300 ttgccactag gggggggcct ttttatatgg ccaagccaag ctctccacgt
cggttgggct 3360 gcacccaaca ataaatgggt agggttgcac caacaaaggg
atgggatggg gggtagaaga 3420 tacgaggata acggggctca atggcacaaa
taagaacgaa tactgccatt aagactcgtg 3480 atccagcgac tgacaccatt
gcatcatcta agggcctcaa aactacctcg gaactgctgc 3540 gctgatctgg
acaccacaga ggttccgagc actttaggtt gcaccaaatg tcccaccagg 3600
tgcaggcaga aaacgctgga acagcgtgta cagtttgtct taacaaaaag tgagggcgct
3660 gaggtcgagc agggtggtgt gacttgttat agcctttaga gctgcgaaag
cgcgtatgga 3720 tttggctcat caggccagat tgagggtctg tggacacatg
tcatgttagt gtacttcaat 3780 cgccccctgg atatagcccc gacaataggc
cgtggcctca tttttttgcc ttccgcacat 3840 ttccattgct cggtacccac
accttgcttc tcctgcactt gccaacctta atactggttt 3900 acattgacca
acatcttaca agcggggggc ttgtctaggg tatatataaa cagtggctct 3960
cccaatcggt tgccagtctc ttttttcctt tctttcccca cagattcgaa atctaaacta
4020 cacatcacac aatgcctgtt actgacgtcc ttaagcgaaa gtccggtgtc
atcgtcggcg 4080 acgatgtccg agccgtgagt atccacgaca agatcagtgt
cgagacgacg cgttttgtgt 4140 aatgacacaa tccgaaagtc gctagcaaca
cacactctct acacaaacta acccagctct 4200 ccatggtgaa gtccaagcga
caggctctgc ccctcaccat cgacggaact acctacgacg 4260 tctccgcttg
ggtgaacttc caccctggtg gagctgaaat cattgagaac taccagggac 4320
gagatgctac tgacgccttc atggttatgc actctcagga agccttcgac aagctcaagc
4380 gaatgcccaa gatcaacccc tcctccgagc tgcctcccca ggctgccgtc
aacgaagctc 4440 aggaggattt ccgaaagctc cgagaagagc tgatcgccac
tggcatgttt gacgcctctc 4500 ccctctggta ctcgtacaag atctccacca
ccctgggtct tggcgtgctt ggatacttcc 4560 tgatggtcca gtaccagatg
tacttcattg gtgctgtgct gctcggtatg cactaccagc 4620 aaatgggatg
gctgtctcat gacatctgcc accaccagac cttcaagaac cgaaactgga 4680
ataacctcgt gggtctggtc tttggcaacg gactccaggg cttctccgtg acctggtgga
4740 aggacagaca caacgcccat cattctgcta ccaacgttca gggtcacgat
cccgacattg 4800 ataacctgcc tctgctcgcc tggtccgagg acgatgtcac
tcgagcttct cccatctccc 4860 gaaagctcat tcagttccaa cagtactatt
tcctggtcat ctgtattctc ctgcgattca 4920 tctggtgttt ccagtctgtg
ctgaccgttc gatccctcaa ggaccgagac aaccagttct 4980 accgatctca
gtacaagaaa gaggccattg gactcgctct gcactggact ctcaagaccc 5040
tgttccacct cttctttatg ccctccatcc tgacctcgct cctggtgttc tttgtttccg
5100 agctcgtcgg tggcttcgga attgccatcg tggtcttcat gaaccactac
cctctggaga 5160 agatcggtga ttccgtctgg gacggacatg gcttctctgt
gggtcagatc catgagacca 5220 tgaacattcg acgaggcatc attactgact
ggttctttgg aggcctgaac taccagatcg 5280 agcaccatct ctggcccacc
ctgcctcgac acaacctcac tgccgtttcc taccaggtgg 5340 aacagctgtg
ccagaagcac aacctcccct accgaaaccc tctgccccat gaaggtctcg 5400
tcatcctgct ccgatacctg gccgtgttcg ctcgaatggc cgagaagcag cccgctggca
5460 aggctctcta agc 5473 <210> SEQ ID NO 50 <211>
LENGTH: 9513 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pDMW214 <400> SEQUENCE: 50 ggtggagctc cagcttttgt
tccctttagt gagggttaat ttcgagcttg gcgtaatcat 60 ggtcatagct
gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 120
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
180 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
cattaatgaa 240 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
ctcttccgct tcctcgctca 300 ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg 360 taatacggtt atccacagaa
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 420 agcaaaaggc
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 480
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
540 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc 600 tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg ctttctcata 660 gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc 720 acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca 780 acccggtaag
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 840
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
900 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
aaaagagttg 960 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
tggttttttt gtttgcaagc 1020 agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt 1080 ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 1140 ggatcttcac
ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 1200
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
1260 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
actacgatac 1320 gggagggctt accatctggc cccagtgctg caatgatacc
gcgagaccca cgctcaccgg 1380 ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg 1440 caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt 1500 cgccagttaa
tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 1560
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
1620 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta 1680 agttggccgc agtgttatca ctcatggtta tggcagcact
gcataattct cttactgtca 1740 tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat 1800 agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat acgggataat accgcgccac 1860 atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 1920
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
1980 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg 2040 caaaaaaggg aataagggcg acacggaaat gttgaatact
catactcttc ctttttcaat 2100 attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt 2160 agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgcgc 2220 cctgtagcgg
cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2280
ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2340 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
tttagtgctt 2400 tacggcacct cgaccccaaa aaacttgatt agggtgatgg
ttcacgtagt gggccatcgc 2460 cctgatagac ggtttttcgc cctttgacgt
tggagtccac gttctttaat agtggactct 2520 tgttccaaac tggaacaaca
ctcaacccta tctcggtcta ttcttttgat ttataaggga 2580 ttttgccgat
ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 2640
attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
2700 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
ggggatgtgc 2760 tgcaaggcga ttaagttggg taacgccagg gttttcccag
tcacgacgtt gtaaaacgac 2820 ggccagtgaa ttgtaatacg actcactata
gggcgaattg ggtaccgggc cccccctcga 2880 ggtcgatggt gtcgataagc
ttgatatcga attcatgtca cacaaaccga tcttcgcctc 2940 aaggaaacct
aattctacat ccgagagact gccgagatcc agtctacact gattaatttt 3000
cgggccaata atttaaaaaa atcgtgttat ataatattat atgtattata tatatacatc
3060 atgatgatac tgacagtcat gtcccattgc taaatagaca gactccatct
gccgcctcca 3120 actgatgttc tcaatattta aggggtcatc tcgcattgtt
taataataaa cagactccat 3180 ctaccgcctc caaatgatgt tctcaaaata
tattgtatga acttattttt attacttagt 3240 attattagac aacttacttg
ctttatgaaa aacacttcct atttaggaaa caatttataa 3300 tggcagttcg
ttcatttaac aatttatgta gaataaatgt tataaatgcg tatgggaaat 3360
cttaaatatg gatagcataa atgatatctg cattgcctaa ttcgaaatca acagcaacga
3420 aaaaaatccc ttgtacaaca taaatagtca tcgagaaata tcaactatca
aagaacagct 3480 attcacacgt tactattgag attattattg gacgagaatc
acacactcaa ctgtctttct 3540 ctcttctaga aatacaggta caagtatgta
ctattctcat tgttcatact tctagtcatt 3600 tcatcccaca tattccttgg
atttctctcc aatgaatgac attctatctt gcaaattcaa 3660 caattataat
aagatatacc aaagtagcgg tatagtggca atcaaaaagc ttctctggtg 3720
tgcttctcgt atttattttt attctaatga tccattaaag gtatatattt atttcttgtt
3780 atataatcct tttgtttatt acatgggctg gatacataaa ggtattttga
tttaattttt 3840 tgcttaaatt caatcccccc tcgttcagtg tcaactgtaa
tggtaggaaa ttaccatact 3900 tttgaagaag caaaaaaaat gaaagaaaaa
aaaaatcgta tttccaggtt agacgttccg 3960 cagaatctag aatgcggtat
gcggtacatt gttcttcgaa cgtaaaagtt gcgctccctg 4020 agatattgta
catttttgct tttacaagta caagtacatc gtacaactat gtactactgt 4080
tgatgcatcc acaacagttt gttttgtttt tttttgtttt ttttttttct aatgattcat
4140 taccgctatg tatacctact tgtacttgta gtaagccggg ttattggcgt
tcaattaatc 4200 atagacttat gaatctgcac ggtgtgcgct gcgagttact
tttagcttat gcatgctact 4260 tgggtgtaat attgggatct gttcggaaat
caacggatgc tcaaccgatt tcgacagtaa 4320 taatttgaat cgaatcggag
cctaaaatga acccgagtat atctcataaa attctcggtg 4380 agaggtctgt
gactgtcagt acaaggtgcc ttcattatgc cctcaacctt accatacctc 4440
actgaatgta gtgtacctct aaaaatgaaa tacagtgcca aaagccaagg cactgagctc
4500 gtctaacgga cttgatatac aaccaattaa aacaaatgaa aagaaataca
gttctttgta 4560 tcatttgtaa caattaccct gtacaaacta aggtattgaa
atcccacaat attcccaaag 4620 tccacccctt tccaaattgt catgcctaca
actcatatac caagcactaa cctaccaaac 4680 accactaaaa ccccacaaaa
tatatcttac cgaatataca gtaacaagct accaccacac 4740 tcgttgggtg
cagtcgccag cttaaagata tctatccaca tcagccacaa ctcccttcct 4800
ttaataaacc gactacaccc ttggctattg aggttatgag tgaatatact gtagacaaga
4860 cactttcaag aagactgttt ccaaaacgta ccactgtcct ccactacaaa
cacacccaat 4920 ctgcttcttc tagtcaaggt tgctacaccg gtaaattata
aatcatcatt tcattagcag 4980 ggcagggccc tttttataga gtcttataca
ctagcggacc ctgccggtag accaacccgc 5040 aggcgcgtca gtttgctcct
tccatcaatg cgtcgtagaa acgacttact ccttcttgag 5100 cagctccttg
accttgttgg caacaagtct ccgacctcgg aggtggagga agagcctccg 5160
atatcggcgg tagtgatacc agcctcgacg gactccttga cggcagcctc aacagcgtca
5220 ccggcgggct tcatgttaag agagaacttg agcatcatgg cggcagacag
aatggtggca 5280 atggggttga ccttctgctt gccgagatcg ggggcagatc
cgtgacaggg ctcgtacaga 5340 ccgaacgcct cgttggtgtc gggcagagaa
gccagagagg cggagggcag cagacccaga 5400 gaaccgggga tgacggaggc
ctcgtcggag atgatatcgc caaacatgtt ggtggtgatg 5460 atgataccat
tcatcttgga gggctgcttg atgaggatca tggcggccga gtcgatcagc 5520
tggtggttga gctcgagctg ggggaattcg tccttgagga ctcgagtgac agtctttcgc
5580 caaagtcgag aggaggccag cacgttggcc ttgtcaagag accacacggg
aagagggggg 5640 ttgtgctgaa gggccaggaa ggcggccatt cgggcaattc
gctcaacctc aggaacggag 5700 taggtctcgg tgtcggaagc gacgccagat
ccgtcatcct cctttcgctc tccaaagtag 5760 atacctccga cgagctctcg
gacaatgatg aagtcggtgc cctcaacgtt tcggatgggg 5820 gagagatcgg
cgagcttggg cgacagcagc tggcagggtc gcaggttggc gtacaggttc 5880
aggtcctttc gcagcttgag gagaccctgc tcgggtcgca cgtcggttcg tccgtcggga
5940 gtggtccata cggtgttggc agcgcctccg acagcaccga gcataataga
gtcagccttt 6000 cggcagatgt cgagagtagc gtcggtgatg ggctcgccct
ccttctcaat ggcagctcct 6060 ccaatgagtc ggtcctcaaa cacaaactcg
gtgccggagg cctcagcaac agacttgagc 6120 accttgacgg cctcggcaat
cacctcgggg ccacagaagt cgccgccgag aagaacaatc 6180 ttcttggagt
cagtcttggt cttcttagtt tcgggttcca ttgtggatgt gtgtggttgt 6240
atgtgtgatg tggtgtgtgg agtgaaaatc tgtggctggc aaacgctctt gtatatatac
6300 gcacttttgc ccgtgctatg tggaagacta aacctccgaa gattgtgact
caggtagtgc 6360 ggtatcggct agggacccaa accttgtcga tgccgatagc
gctatcgaac gtaccccagc 6420 cggccgggag tatgtcggag gggacatacg
agatcgtcaa gggtttgtgg ccaactggta 6480 aataaatgat gtcgacgttt
aaacagtgta cgcagtacta tagaggaaca attgccccgg 6540 agaagacggc
caggccgcct agatgacaaa ttcaacaact cacagctgac tttctgccat 6600
tgccactagg ggggggcctt tttatatggc caagccaagc tctccacgtc ggttgggctg
6660 cacccaacaa taaatgggta gggttgcacc aacaaaggga tgggatgggg
ggtagaagat 6720 acgaggataa cggggctcaa tggcacaaat aagaacgaat
actgccatta agactcgtga 6780 tccagcgact gacaccattg catcatctaa
gggcctcaaa actacctcgg aactgctgcg 6840 ctgatctgga caccacagag
gttccgagca ctttaggttg caccaaatgt cccaccaggt 6900 gcaggcagaa
aacgctggaa cagcgtgtac agtttgtctt aacaaaaagt gagggcgctg 6960
aggtcgagca gggtggtgtg acttgttata gcctttagag ctgcgaaagc gcgtatggat
7020 ttggctcatc aggccagatt gagggtctgt ggacacatgt catgttagtg
tacttcaatc 7080 gccccctgga tatagccccg acaataggcc gtggcctcat
ttttttgcct tccgcacatt 7140 tccattgctc ggtacccaca ccttgcttct
cctgcacttg ccaaccttaa tactggttta 7200 cattgaccaa catcttacaa
gcggggggct tgtctagggt atatataaac agtggctctc 7260 ccaatcggtt
gccagtctct tttttccttt ctttccccac agattcgaaa tctaaactac 7320
acatcacaca atgcctgtta ctgacgtcct taagcgaaag tccggtgtca tcgtcggcga
7380 cgatgtccga gccgtgagta tccacgacaa gatcagtgtc gagacgacgc
gttttgtgta 7440 atgacacaat ccgaaagtcg ctagcaacac acactctcta
cacaaactaa cccagctctc 7500 catggcatgg atggtacgtc ctgtagaaac
cccaacccgt gaaatcaaaa aactcgacgg 7560 cctgtgggca ttcagtctgg
atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag 7620 cgcgttacaa
gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga 7680
tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
7740 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
acggcaaagt 7800 gtgggtcaat aatcaggaag tgatggagca tcagggcggc
tatacgccat ttgaagccga 7860 tgtcacgccg tatgttattg ccgggaaaag
tgtacgtatc accgtttgtg tgaacaacga 7920 actgaactgg cagactatcc
cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa 7980 gcagtcttac
ttccatgatt tctttaacta tgccgggatc catcgcagcg taatgctcta 8040
caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
8100 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
ttgaactgcg 8160 tgatgcggat caacaggtgg ttgcaactgg acaaggcact
agcgggactt tgcaagtggt 8220 gaatccgcac ctctggcaac cgggtgaagg
ttatctctat gaactgtgcg tcacagccaa 8280 aagccagaca gagtgtgata
tctacccgct tcgcgtcggc atccggtcag tggcagtgaa 8340 gggcgaacag
ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga 8400
agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
8460 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
ctgaagagat 8520 gctcgactgg gcagatgaac atggcatcgt ggtgattgat
gaaactgctg ctgtcggctt 8580 taacctctct ttaggcattg gtttcgaagc
gggcaacaag ccgaaagaac tgtacagcga 8640 agaggcagtc aacggggaaa
ctcagcaagc gcacttacag gcgattaaag agctgatagc 8700 gcgtgacaaa
aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg 8760
tccgcaagtg cacgggaata tttcgccact ggcggaagca acgcgtaaac tcgacccgac
8820 gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata
ccatcagcga 8880 tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
tatgtccaaa gcggcgattt 8940 ggaaacggca gagaaggtac tggaaaaaga
acttctggcc tggcaggaga aactgcatca 9000 gccgattatc atcaccgaat
acggcgtgga tacgttagcc gggctgcact caatgtacac 9060 cgacatgtgg
agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga 9120
tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca
9180 aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc
gcaaaccgaa 9240 gtcggcggct tttctgctgc aaaaacgctg gactggcatg
aacttcggtg aaaaaccgca 9300 gcagggaggc aaacaatgat taattaacta
gagcggccgc caccgcggcc cgagattccg 9360 gcctcttcgg ccgccaagcg
acccgggtgg acgtctagag gtacctagca attaacagat 9420 agtttgccgg
tgataattct cttaacctcc cacactcctt tgacataacg atttatgtaa 9480
cgaaactgaa atttgaccag atattgtgtc cgc 9513 <210> SEQ ID NO 51
<211> LENGTH: 8910 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pFmD8S <400> SEQUENCE: 51
catggtgaag tccaagcgac aggctctgcc cctcaccatc gacggaacta cctacgacgt
60 ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact
accagggacg 120 agatgctact gacgccttca tggttatgca ctctcaggaa
gccttcgaca agctcaagcg 180 aatgcccaag atcaacccct cctccgagct
gcctccccag gctgccgtca acgaagctca 240 ggaggatttc cgaaagctcc
gagaagagct gatcgccact ggcatgtttg acgcctctcc 300 cctctggtac
tcgtacaaga tctccaccac cctgggtctt ggcgtgcttg gatacttcct 360
gatggtccag taccagatgt acttcattgg tgctgtgctg ctcggtatgc actaccagca
420 aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc
gaaactggaa 480 taacctcgtg ggtctggtct ttggcaacgg actccagggc
ttctccgtga cctggtggaa 540 ggacagacac aacgcccatc attctgctac
caacgttcag ggtcacgatc ccgacattga 600 taacctgcct ctgctcgcct
ggtccgagga cgatgtcact cgagcttctc ccatctcccg 660 aaagctcatt
cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat 720
ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta
780 ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc
tcaagaccct 840 gttccacctc ttctttatgc cctccatcct gacctcgctc
ctggtgttct ttgtttccga 900 gctcgtcggt ggcttcggaa ttgccatcgt
ggtcttcatg aaccactacc ctctggagaa 960 gatcggtgat tccgtctggg
acggacatgg cttctctgtg ggtcagatcc atgagaccat 1020 gaacattcga
cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga 1080
gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga
1140 acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg
aaggtctcgt 1200 catcctgctc cgatacctgg ccgtgttcgc tcgaatggcc
gagaagcagc ccgctggcaa 1260 ggctctctaa gcggccgcca ccgcggcccg
agattccggc ctcttcggcc gccaagcgac 1320 ccgggtggac gtctagaggt
acctagcaat taacagatag tttgccggtg ataattctct 1380 taacctccca
cactcctttg acataacgat ttatgtaacg aaactgaaat ttgaccagat 1440
attgtgtccg cggtggagct ccagcttttg ttccctttag tgagggttaa tttcgagctt
1500 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
caattccaca 1560 caacatacga gccggaagca taaagtgtaa agcctggggt
gcctaatgag tgagctaact 1620 cacattaatt gcgttgcgct cactgcccgc
tttccagtcg ggaaacctgt cgtgccagct 1680 gcattaatga atcggccaac
gcgcggggag aggcggtttg cgtattgggc gctcttccgc 1740 ttcctcgctc
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1800
ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1860 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
cgtttttcca 1920 taggctccgc ccccctgacg agcatcacaa aaatcgacgc
tcaagtcaga ggtggcgaaa 1980 cccgacagga ctataaagat accaggcgtt
tccccctgga agctccctcg tgcgctctcc 2040 tgttccgacc ctgccgctta
ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2100 gctttctcat
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2160
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2220 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
ctggtaacag 2280 gattagcaga gcgaggtatg taggcggtgc tacagagttc
ttgaagtggt ggcctaacta 2340 cggctacact agaaggacag tatttggtat
ctgcgctctg ctgaagccag ttaccttcgg 2400 aaaaagagtt ggtagctctt
gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2460 tgtttgcaag
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 2520
ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2580 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
ttaaatcaat 2640 ctaaagtata tatgagtaaa cttggtctga cagttaccaa
tgcttaatca gtgaggcacc 2700 tatctcagcg atctgtctat ttcgttcatc
catagttgcc tgactccccg tcgtgtagat 2760 aactacgata cgggagggct
taccatctgg ccccagtgct gcaatgatac cgcgagaccc 2820 acgctcaccg
gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 2880
aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2940 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
caggcatcgt 3000 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
ggttcccaac gatcaaggcg 3060 agttacatga tcccccatgt tgtgcaaaaa
agcggttagc tccttcggtc ctccgatcgt 3120 tgtcagaagt aagttggccg
cagtgttatc actcatggtt atggcagcac tgcataattc 3180 tcttactgtc
atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3240
attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
3300 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
cttcggggcg 3360 aaaactctca aggatcttac cgctgttgag atccagttcg
atgtaaccca ctcgtgcacc 3420 caactgatct tcagcatctt ttactttcac
cagcgtttct gggtgagcaa aaacaggaag 3480 gcaaaatgcc gcaaaaaagg
gaataagggc gacacggaaa tgttgaatac tcatactctt 3540 cctttttcaa
tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3600
tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3660 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
cgcgcagcgt 3720 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
gctttcttcc cttcctttct 3780 cgccacgttc gccggctttc cccgtcaagc
tctaaatcgg gggctccctt tagggttccg 3840 atttagtgct ttacggcacc
tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3900 tgggccatcg
ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3960
tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
4020 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
tttaacaaaa 4080 atttaacgcg aattttaaca aaatattaac gcttacaatt
tccattcgcc attcaggctg 4140 cgcaactgtt gggaagggcg atcggtgcgg
gcctcttcgc tattacgcca gctggcgaaa 4200 gggggatgtg ctgcaaggcg
attaagttgg gtaacgccag ggttttccca gtcacgacgt 4260 tgtaaaacga
cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg 4320
ccccccctcg aggtcgatgg tgtcgataag cttgatatcg aattcatgtc acacaaaccg
4380 atcttcgcct caaggaaacc taattctaca tccgagagac tgccgagatc
cagtctacac 4440 tgattaattt tcgggccaat aatttaaaaa aatcgtgtta
tataatatta tatgtattat 4500 atatatacat catgatgata ctgacagtca
tgtcccattg ctaaatagac agactccatc 4560 tgccgcctcc aactgatgtt
ctcaatattt aaggggtcat ctcgcattgt ttaataataa 4620 acagactcca
tctaccgcct ccaaatgatg ttctcaaaat atattgtatg aacttatttt 4680
tattacttag tattattaga caacttactt gctttatgaa aaacacttcc tatttaggaa
4740 acaatttata atggcagttc gttcatttaa caatttatgt agaataaatg
ttataaatgc 4800 gtatgggaaa tcttaaatat ggatagcata aatgatatct
gcattgccta attcgaaatc 4860 aacagcaacg aaaaaaatcc cttgtacaac
ataaatagtc atcgagaaat atcaactatc 4920 aaagaacagc tattcacacg
ttactattga gattattatt ggacgagaat cacacactca 4980 actgtctttc
tctcttctag aaatacaggt acaagtatgt actattctca ttgttcatac 5040
ttctagtcat ttcatcccac atattccttg gatttctctc caatgaatga cattctatct
5100 tgcaaattca acaattataa taagatatac caaagtagcg gtatagtggc
aatcaaaaag 5160 cttctctggt gtgcttctcg tatttatttt tattctaatg
atccattaaa ggtatatatt 5220 tatttcttgt tatataatcc ttttgtttat
tacatgggct ggatacataa aggtattttg 5280 atttaatttt ttgcttaaat
tcaatccccc ctcgttcagt gtcaactgta atggtaggaa 5340 attaccatac
ttttgaagaa gcaaaaaaaa tgaaagaaaa aaaaaatcgt atttccaggt 5400
tagacgttcc gcagaatcta gaatgcggta tgcggtacat tgttcttcga acgtaaaagt
5460 tgcgctccct gagatattgt acatttttgc ttttacaagt acaagtacat
cgtacaacta 5520 tgtactactg ttgatgcatc cacaacagtt tgttttgttt
ttttttgttt tttttttttc 5580 taatgattca ttaccgctat gtatacctac
ttgtacttgt agtaagccgg gttattggcg 5640 ttcaattaat catagactta
tgaatctgca cggtgtgcgc tgcgagttac ttttagctta 5700 tgcatgctac
ttgggtgtaa tattgggatc tgttcggaaa tcaacggatg ctcaaccgat 5760
ttcgacagta ataatttgaa tcgaatcgga gcctaaaatg aacccgagta tatctcataa
5820 aattctcggt gagaggtctg tgactgtcag tacaaggtgc cttcattatg
ccctcaacct 5880 taccatacct cactgaatgt agtgtacctc taaaaatgaa
atacagtgcc aaaagccaag 5940 gcactgagct cgtctaacgg acttgatata
caaccaatta aaacaaatga aaagaaatac 6000 agttctttgt atcatttgta
acaattaccc tgtacaaact aaggtattga aatcccacaa 6060 tattcccaaa
gtccacccct ttccaaattg tcatgcctac aactcatata ccaagcacta 6120
acctaccaaa caccactaaa accccacaaa atatatctta ccgaatatac agtaacaagc
6180 taccaccaca ctcgttgggt gcagtcgcca gcttaaagat atctatccac
atcagccaca 6240 actcccttcc tttaataaac cgactacacc cttggctatt
gaggttatga gtgaatatac 6300 tgtagacaag acactttcaa gaagactgtt
tccaaaacgt accactgtcc tccactacaa 6360 acacacccaa tctgcttctt
ctagtcaagg ttgctacacc ggtaaattat aaatcatcat 6420 ttcattagca
gggcagggcc ctttttatag agtcttatac actagcggac cctgccggta 6480
gaccaacccg caggcgcgtc agtttgctcc ttccatcaat gcgtcgtaga aacgacttac
6540 tccttcttga gcagctcctt gaccttgttg gcaacaagtc tccgacctcg
gaggtggagg 6600 aagagcctcc gatatcggcg gtagtgatac cagcctcgac
ggactccttg acggcagcct 6660 caacagcgtc accggcgggc ttcatgttaa
gagagaactt gagcatcatg gcggcagaca 6720 gaatggtggc aatggggttg
accttctgct tgccgagatc gggggcagat ccgtgacagg 6780 gctcgtacag
accgaacgcc tcgttggtgt cgggcagaga agccagagag gcggagggca 6840
gcagacccag agaaccgggg atgacggagg cctcgtcgga gatgatatcg ccaaacatgt
6900 tggtggtgat gatgatacca ttcatcttgg agggctgctt gatgaggatc
atggcggccg 6960 agtcgatcag ctggtggttg agctcgagct gggggaattc
gtccttgagg actcgagtga 7020 cagtctttcg ccaaagtcga gaggaggcca
gcacgttggc cttgtcaaga gaccacacgg 7080 gaagaggggg gttgtgctga
agggccagga aggcggccat tcgggcaatt cgctcaacct 7140 caggaacgga
gtaggtctcg gtgtcggaag cgacgccaga tccgtcatcc tcctttcgct 7200
ctccaaagta gatacctccg acgagctctc ggacaatgat gaagtcggtg ccctcaacgt
7260 ttcggatggg ggagagatcg gcgagcttgg gcgacagcag ctggcagggt
cgcaggttgg 7320 cgtacaggtt caggtccttt cgcagcttga ggagaccctg
ctcgggtcgc acgtcggttc 7380 gtccgtcggg agtggtccat acggtgttgg
cagcgcctcc gacagcaccg agcataatag 7440 agtcagcctt tcggcagatg
tcgagagtag cgtcggtgat gggctcgccc tccttctcaa 7500 tggcagctcc
tccaatgagt cggtcctcaa acacaaactc ggtgccggag gcctcagcaa 7560
cagacttgag caccttgacg gcctcggcaa tcacctcggg gccacagaag tcgccgccga
7620 gaagaacaat cttcttggag tcagtcttgg tcttcttagt ttcgggttcc
attgtggatg 7680 tgtgtggttg tatgtgtgat gtggtgtgtg gagtgaaaat
ctgtggctgg caaacgctct 7740 tgtatatata cgcacttttg cccgtgctat
gtggaagact aaacctccga agattgtgac 7800 tcaggtagtg cggtatcggc
tagggaccca aaccttgtcg atgccgatag cgctatcgaa 7860 cgtaccccag
ccggccggga gtatgtcgga ggggacatac gagatcgtca agggtttgtg 7920
gccaactggt aaataaatga tgtcgacgtt taaacagtgt acgcagatct actatagagg
7980 aacatttaaa ttgccccgga gaagacggcc aggccgccta gatgacaaat
tcaacaactc 8040 acagctgact ttctgccatt gccactaggg gggggccttt
ttatatggcc aagccaagct 8100 ctccacgtcg gttgggctgc acccaacaat
aaatgggtag ggttgcacca acaaagggat 8160 gggatggggg gtagaagata
cgaggataac ggggctcaat ggcacaaata agaacgaata 8220 ctgccattaa
gactcgtgat ccagcgactg acaccattgc atcatctaag ggcctcaaaa 8280
ctacctcgga actgctgcgc tgatctggac accacagagg ttccgagcac tttaggttgc
8340 accaaatgtc ccaccaggtg caggcagaaa acgctggaac agcgtgtaca
gtttgtctta 8400 acaaaaagtg agggcgctga ggtcgagcag ggtggtgtga
cttgttatag cctttagagc 8460 tgcgaaagcg cgtatggatt tggctcatca
ggccagattg agggtctgtg gacacatgtc 8520 atgttagtgt acttcaatcg
ccccctggat atagccccga caataggccg tggcctcatt 8580 tttttgcctt
ccgcacattt ccattgctcg gtacccacac cttgcttctc ctgcacttgc 8640
caaccttaat actggtttac attgaccaac atcttacaag cggggggctt gtctagggta
8700 tatataaaca gtggctctcc caatcggttg ccagtctctt ttttcctttc
tttccccaca 8760 gattcgaaat ctaaactaca catcacagaa ttccgagccg
tgagtatcca cgacaagatc 8820 agtgtcgaga cgacgcgttt tgtgtaatga
cacaatccga aagtcgctag caacacacac 8880 tctctacaca aactaaccca
gctctggtac 8910 <210> SEQ ID NO 52 <211> LENGTH: 1272
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(1270) <223> OTHER INFORMATION: synthetic
delta-8 desaturase CDS, codon-optimized for Yarrowia lipolytica
("D8SF" or "EgD8S") <300> PUBLICATION INFORMATION:
<302> TITLE: DELTA-8 DESATURASE AND ITS USE IN MAKING
POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT NUMBER: WO
2006/012325 and WO 2006/012326 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2006-02-02 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(1272) <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-8 DESATURASE AND ITS USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: US-2005-0287652-A1 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2005-12-29 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(1272) <400> SEQUENCE:
52 catggtgaag tccaagcgac aggctctgcc cctcaccatc gacggaacta
cctacgacgt 60 ctccgcttgg gtgaacttcc accctggtgg agctgaaatc
attgagaact accagggacg 120 agatgctact gacgccttca tggttatgca
ctctcaggaa gccttcgaca agctcaagcg 180 aatgcccaag atcaacccct
cctccgagct gcctccccag gctgccgtca acgaagctca 240 ggaggatttc
cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc 300
cctctggtac tcgtacaaga tctccaccac cctgggtctt ggcgtgcttg gatacttcct
360 gatggtccag taccagatgt acttcattgg tgctgtgctg ctcggtatgc
actaccagca 420 aatgggatgg ctgtctcatg acatctgcca ccaccagacc
ttcaagaacc gaaactggaa 480 taacctcgtg ggtctggtct ttggcaacgg
actccagggc ttctccgtga cctggtggaa 540 ggacagacac aacgcccatc
attctgctac caacgttcag ggtcacgatc ccgacattga 600 taacctgcct
ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg 660
aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat
720 ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca
accagttcta 780 ccgatctcag tacaagaaag aggccattgg actcgctctg
cactggactc tcaagaccct 840 gttccacctc ttctttatgc cctccatcct
gacctcgctc ctggtgttct ttgtttccga 900 gctcgtcggt ggcttcggaa
ttgccatcgt ggtcttcatg aaccactacc ctctggagaa 960 gatcggtgat
tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat 1020
gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga
1080 gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct
accaggtgga 1140 acagctgtgc cagaagcaca acctccccta ccgaaaccct
ctgccccatg aaggtctcgt 1200 catcctgctc cgatacctgg ccgtgttcgc
tcgaatggcc gagaagcagc ccgctggcaa 1260 ggctctctaa gc 1272
<210> SEQ ID NO 53 <211> LENGTH: 422 <212> TYPE:
PRT <213> ORGANISM: Euglena gracilis <220> FEATURE:
<221> NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION:
synthetic delta-8 desaturase codon-optimized for Yarrowia
lipolytica ("D8SF" or "EgD8S") <300> PUBLICATION INFORMATION:
<302> TITLE: DELTA-8 DESATURASE AND ITS USE IN MAKING
POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT NUMBER: WO
2006/012325 and WO 2006/012326 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2006-02-02 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(422) <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-8 DESATURASE AND ITS USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: US-2005-0287652-A1 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2005-12-29 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(422) <400> SEQUENCE: 53
Met Val Lys Ser Lys Arg Gln Ala Leu Pro Leu Thr Ile Asp Gly Thr 1 5
10 15 Thr Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala
Glu 20 25 30 Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala
Phe Met Val 35 40 45 Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys
Arg Met Pro Lys Ile 50 55 60 Asn Pro Ser Ser Glu Leu Pro Pro Gln
Ala Ala Val Asn Glu Ala Gln 65 70 75 80 Glu Asp Phe Arg Lys Leu Arg
Glu Glu Leu Ile Ala Thr Gly Met Phe 85 90 95 Asp Ala Ser Pro Leu
Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly 100 105 110 Leu Gly Val
Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe 115 120 125 Ile
Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 130 135
140 Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn
145 150 155 160 Asn Leu Val Gly Leu Val Phe Gly Asn Gly Leu Gln Gly
Phe Ser Val 165 170 175 Thr Trp Trp Lys Asp Arg His Asn Ala His His
Ser Ala Thr Asn Val 180 185 190 Gln Gly His Asp Pro Asp Ile Asp Asn
Leu Pro Leu Leu Ala Trp Ser 195 200 205 Glu Asp Asp Val Thr Arg Ala
Ser Pro Ile Ser Arg Lys Leu Ile Gln 210 215 220 Phe Gln Gln Tyr Tyr
Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 225 230 235 240 Trp Cys
Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 245 250 255
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 260
265 270 Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro
Ser 275 280 285 Ile Leu Thr Ser Leu Leu Val Phe Phe Val Ser Glu Leu
Val Gly Gly 290 295 300 Phe Gly Ile Ala Ile Val Val Phe Met Asn His
Tyr Pro Leu Glu Lys 305 310 315 320 Ile Gly Asp Ser Val Trp Asp Gly
His Gly Phe Ser Val Gly Gln Ile 325 330 335 His Glu Thr Met Asn Ile
Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 340 345 350 Gly Gly Leu Asn
Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 355 360 365 Arg His
Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 370 375 380
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 385
390 395 400 Ile Leu Leu Arg Tyr Leu Ala Val Phe Ala Arg Met Ala Glu
Lys Gln 405 410 415 Pro Ala Gly Lys Ala Leu 420 <210> SEQ ID
NO 54 <211> LENGTH: 27 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PUD17-F <400> SEQUENCE: 54
aagatcccat ggcttcttcc actgttg 27 <210> SEQ ID NO 55
<211> LENGTH: 33 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PUD17-R <400> SEQUENCE: 55 atcatcgcgg
ccgcctagtt ggccttggtc ttg 33 <210> SEQ ID NO 56 <211>
LENGTH: 12649 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Plasmid pKUNF12T6E <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (2507)..(2507)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(2512)..(2515) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 56 taaccctcac taaagggaac aaaagctgga
gctccaccgc ggacacaata tctggtcaaa 60 tttcagtttc gttacataaa
tcgttatgtc aaaggagtgt gggaggttaa gagaattatc 120 accggcaaac
tatctgttaa ttgctaggta cctctagacg tccacccggg tcgcttggcg 180
gccgaagagg ccggaatctc gggccgcggt ggcggccgct tagttggtct tggacttctt
240 gggcttcttc aggtaggact ggacaaagaa gttgccgaac agagcgagca
gggtgatcat 300 gtacacgccg agcagctgga ccagagcctg agggtagtcg
caggggaaga ggtagtcgta 360 cagggactgc accagcatag ccatgaactg
ggtcatctgc agagtggtga tgtagggctt 420 gatgggcttg acgaagccga
agccctgaga ggaaaagaag tagtaggcgt acatgacggt 480 gtggacgaag
gagttgagga tgacggagaa gtaggcgtcg ccaccaggag cgtacttggc 540
aatagcccac cagatggcga agatggtggc atggtggtac acgtgcagga aggagacctg
600 gttgaacttc ttgcacagga tcatgatagc ggtgtccagg aactcgtagg
ccttggagac 660 gtagaacacg tagacgattc gggacatgcc ctgagcgtgg
gactcgttgc ccttctccat 720 gtcgttgccg aagaccttgt agccacccag
gatagcctgt cggatggtct cgacgcacat 780 gtagagggac agtccgaaga
ggaacaggtt gtggagcagc ttgatggtct tcagctcgaa 840 gggcttctcc
atctgcttca tgatgggaat gccgaagagc agcatggcca tgtagccgac 900
ctcgaaggcg agcatggtgg agacgtccat catgggcaga ccgtcggtca gagcgtaggg
960 cttagctccg tccatccact ggtcgacacc ggtctcgact cgtccgacca
cgtcgtccca 1020 gacagaggag ttggccatgg tgaatgattc ttatactcag
aaggaaatgc ttaacgattt 1080 cgggtgtgag ttgacaagga gagagagaaa
agaagaggaa aggtaattcg gggacggtgg 1140 tcttttatac ccttggctaa
agtcccaacc acaaagcaaa aaaattttca gtagtctatt 1200 ttgcgtccgg
catgggttac ccggatggcc agacaaagaa actagtacaa agtctgaaca 1260
agcgtagatt ccagactgca gtaccctacg cccttaacgg caagtgtggg aaccggggga
1320 ggtttgatat gtggggtgaa gggggctctc gccggggttg ggcccgctac
tgggtcaatt 1380 tggggtcaat tggggcaatt ggggctgttt tttgggacac
aaatacgccg ccaacccggt 1440 ctctcctgaa ttctgcatcg atcgaggaag
aggacaagcg gctgcttctt aagtttgtga 1500 catcagtatc caaggcacca
ttgcaaggat tcaaggcttt gaacccgtca tttgccattc 1560 gtaacgctgg
tagacaggtt gatcggttcc ctacggcctc cacctgtgtc aatcttctca 1620
agctgcctga ctatcaggac attgatcaac ttcggaagaa acttttgtat gccattcgat
1680 cacatgctgg tttcgatttg tcttagagga acgcatatac agtaatcata
gagaataaac 1740 gatattcatt tattaaagta gatagttgag gtagaagttg
taaagagtga taaatagcgg 1800 ccgcgcctac ttaagcaacg ggcttgataa
cagcgggggg ggtgcccacg ttgttgcggt 1860 tgcggaagaa cagaacaccc
ttaccagcac cctcggcacc agcgctgggc tcaacccact 1920 ggcacatacg
cgcactgcgg tacatggcgc ggatgaagcc acgaggacca tcctggacat 1980
cagcccggta gtgcttgccc atgatgggct taatggcctc ggtggcctcg tccgcgttgt
2040 agaaggggat gctgctgacg tagtggtgga ggacatgagt ctcgatgatg
ccgtggagaa 2100 ggtggcggcc gatgaagccc atctcacggt caatggtagc
agcggcacca cggacgaagt 2160 tccactcgtc gttggtgtag tggggaaggg
tagggtcggt gtgctggagg aaggtgatgg 2220 caacgagcca gtggttaacc
cagaggtagg gaacaaagta ccagatggcc atgttgtaga 2280 aaccgaactt
ctgaacgagg aagtacagag cagtggccat cagaccgata ccaatatcgc 2340
tgaggacgat gagcttagcg tcactgttct cgtacagagg gctgcgggga tcgaagtggt
2400 taacaccacc gccgaggccg ttatgcttgc ccttgccgcg accctcacgc
tggcgctcgt 2460 ggtagttgtg gccggtaaca ttggtgatga ggtagttggg
ccagccnacg annnnctcag 2520 taagatgagc gagctcgtgg gtcatctttc
cgagacgagt agcctgctgc tcgcgggttc 2580 ggggaacgaa gaccatgtca
cgctccatgt tgccagtggc cttgtggtgc tttcggtggg 2640 agatttgcca
gctgaagtag gggacaagga gggaagagtg aagaacccag ccagtaatgt 2700
cgttgatgat gcgagaatcg gagaaagcac cgtgaccgca ctcatgggca ataacccaga
2760 gaccagtacc gaaaagaccc tgaagaacgg tgtacacggc ccacagacca
gcgcgggcgg 2820 gggtggaggg gatatattcg ggggtcacaa agttgtacca
gatgctgaaa gtggtagtca 2880 ggaggacaat gtcgcggagg atataaccgt
atcccttgag agcggagcgc ttgaagcagt 2940 gcttagggat ggcattgtag
atgtccttga tggtaaagtc gggaacctcg aactggttgc 3000 cgtaggtgtc
gagcatgaca ccatactcgg acttgggctt ggcgatatca acctcggaca 3060
tggacgagag cgatgtggaa gaggccgagt ggcggggaga gtctgaagga gagacggcgg
3120 cagactcaga atccgtcaca gtagttgagg tgacggtgcg tctaagcgca
gggttctgct 3180 tgggcagagc cgaagtggac gccatggaga gctgggttag
tttgtgtaga gagtgtgtgt 3240 tgctagcgac tttcggattg tgtcattaca
caaaacgcgt cgtctcgaca ctgatcttgt 3300 cgtggatact cacggctcgg
acatcgtcgc cgacgatgac accggacttt cgcttaagga 3360 cgtcagtaac
aggcattgtg tgatgtgtag tttagatttc gaatctgtgg ggaaagaaag 3420
gaaaaaagag actggcaacc gattgggaga gccactgttt atatataccc tagacaagcc
3480 ccccgcttgt aagatgttgg tcaatgtaaa ccagtattaa ggttggcaag
tgcaggagaa 3540 gcaaggtgtg ggtaccgagc aatggaaatg tgcggaaggc
aaaaaaatga ggccacggcc 3600 tattgtcggg gctatatcca gggggcgatt
gaagtacact aacatgacat gtgtccacag 3660 accctcaatc tggcctgatg
agccaaatcc atacgcgctt tcgcagctct aaaggctata 3720 acaagtcaca
ccaccctgct cgacctcagc gccctcactt tttgttaaga caaactgtac 3780
acgctgttcc agcgttttct gcctgcacct ggtgggacat ttggtgcaac ctaaagtgct
3840 cggaacctct gtggtgtcca gatcagcgca gcagttccga ggtagttttg
aggcccttag 3900 atgatgcaat ggtgtcagtc gctggatcac gagtcttaat
ggcagtattc gttcttattt 3960 gtgccattga gccccgttat cctcgtatct
tctacccccc atcccatccc tttgttggtg 4020 caaccctacc catttattgt
tgggtgcagc ccaaccgacg tggagagctt ggcttggcca 4080 tataaaaagg
ccccccccta gtggcaatgg cagaaagtca gctgtgagtt gttgaatttg 4140
tcatctaggc ggcctggccg tcttctccgg ggcaattgtt cctctatagt actgcgtaca
4200 ctgtttaaac agtgtacgca gatctgcgac gacggaattc ctgcagccca
tctgcagaat 4260 tcaggagaga ccgggttggc ggcgtatttg tgtcccaaaa
aacagcccca attgccccaa 4320 ttgaccccaa attgacccag tagcgggccc
aaccccggcg agagccccct tcaccccaca 4380 tatcaaacct cccccggttc
ccacacttgc cgttaagggc gtagggtact gcagtctgga 4440 atctacgctt
gttcagactt tgtactagtt tctttgtctg gccatccggg taacccatgc 4500
cggacgcaaa atagactact gaaaattttt ttgctttgtg gttgggactt tagccaaggg
4560 tataaaagac caccgtcccc gaattacctt tcctcttctt ttctctctct
ccttgtcaac 4620 tcacacccga aatcgttaag catttccttc tgagtataag
aatcattcac catggctgcc 4680 gctccctctg tgcgaacctt tacccgagcc
gaggttctga acgctgaggc tctgaacgag 4740 ggcaagaagg acgctgaggc
tcccttcctg atgatcatcg acaacaaggt gtacgacgtc 4800 cgagagttcg
tccctgacca tcctggaggc tccgtgattc tcacccacgt tggcaaggac 4860
ggcaccgacg tctttgacac ctttcatccc gaggctgctt gggagactct cgccaacttc
4920 tacgttggag acattgacga gtccgaccga gacatcaaga acgatgactt
tgccgctgag 4980 gtccgaaagc tgcgaaccct gttccagtct ctcggctact
acgactcctc taaggcctac 5040 tacgccttca aggtctcctt caacctctgc
atctggggac tgtccaccgt cattgtggcc 5100 aagtggggtc agacctccac
cctcgccaac gtgctctctg ctgccctgct cggcctgttc 5160 tggcagcagt
gcggatggct ggctcacgac tttctgcacc accaggtctt ccaggaccga 5220
ttctggggtg atctcttcgg agccttcctg ggaggtgtct gccagggctt ctcctcttcc
5280 tggtggaagg acaagcacaa cactcaccat gccgctccca acgtgcatgg
cgaggatcct 5340 gacattgaca cccaccctct cctgacctgg tccgagcacg
ctctggagat gttctccgac 5400 gtccccgatg aggagctgac ccgaatgtgg
tctcgattca tggtcctgaa ccagacctgg 5460 ttctacttcc ccattctctc
cttcgctcga ctgtcttggt gcctccagtc cattctcttt 5520 gtgctgccca
acggtcaggc tcacaagccc tccggagctc gagtgcccat ctccctggtc 5580
gagcagctgt ccctcgccat gcactggacc tggtacctcg ctaccatgtt cctgttcatc
5640 aaggatcctg tcaacatgct cgtgtacttc ctggtgtctc aggctgtgtg
cggaaacctg 5700 ctcgccatcg tgttctccct caaccacaac ggtatgcctg
tgatctccaa ggaggaggct 5760 gtcgacatgg atttctttac caagcagatc
atcactggtc gagatgtcca tcctggactg 5820 ttcgccaact ggttcaccgg
tggcctgaac taccagatcg agcatcacct gttcccttcc 5880 atgcctcgac
acaacttctc caagatccag cctgccgtcg agaccctgtg caagaagtac 5940
aacgtccgat accacaccac tggtatgatc gagggaactg ccgaggtctt ctcccgactg
6000 aacgaggtct ccaaggccac ctccaagatg ggcaaggctc agtaagcggc
cgcatgagaa 6060 gataaatata taaatacatt gagatattaa atgcgctaga
ttagagagcc tcatactgct 6120 cggagagaag ccaagacgag tactcaaagg
ggattacacc atccatatcc acagacacaa 6180 gctggggaaa ggttctatat
acactttccg gaataccgta gtttccgatg ttatcaatgg 6240 gggcagccag
gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca 6300
tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga
6360 acttgaagtg aaggaattta aattgccccg gagaagacgg ccaggccgcc
tagatgacaa 6420 attcaacaac tcacagctga ctttctgcca ttgccactag
gggggggcct ttttatatgg 6480 ccaagccaag ctctccacgt cggttgggct
gcacccaaca ataaatgggt agggttgcac 6540 caacaaaggg atgggatggg
gggtagaaga tacgaggata acggggctca atggcacaaa 6600 taagaacgaa
tactgccatt aagactcgtg atccagcgac tgacaccatt gcatcatcta 6660
agggcctcaa aactacctcg gaactgctgc gctgatctgg acaccacaga ggttccgagc
6720 actttaggtt gcaccaaatg tcccaccagg tgcaggcaga aaacgctgga
acagcgtgta 6780 cagtttgtct taacaaaaag tgagggcgct gaggtcgagc
agggtggtgt gacttgttat 6840 agcctttaga gctgcgaaag cgcgtatgga
tttggctcat caggccagat tgagggtctg 6900 tggacacatg tcatgttagt
gtacttcaat cgccccctgg atatagcccc gacaataggc 6960 cgtggcctca
tttttttgcc ttccgcacat ttccattgct cggtacccac accttgcttc 7020
tcctgcactt gccaacctta atactggttt acattgacca acatcttaca agcggggggc
7080 ttgtctaggg tatatataaa cagtggctct cccaatcggt tgccagtctc
ttttttcctt 7140 tctttcccca cagattcgaa atctaaacta cacatcacac
aatgcctgtt actgacgtcc 7200 ttaagcgaaa gtccggtgtc atcgtcggcg
acgatgtccg agccgtgagt atccacgaca 7260 agatcagtgt cgagacgacg
cgttttgtgt aatgacacaa tccgaaagtc gctagcaaca 7320 cacactctct
acacaaacta acccagctct ccatggagtc cattgctccc ttcctgccct 7380
ccaagatgcc tcaggacctg ttcatggacc tcgccagcgc tatcggtgtc cgagctgctc
7440 cctacgtcga tcccctggag gctgccctgg ttgcccaggc cgagaagtac
attcccacca 7500 ttgtccatca cactcgaggc ttcctggttg ccgtggagtc
tcccctggct cgagagctgc 7560 ctctgatgaa ccccttccac gtgctcctga
tcgtgctcgc ctacctggtc accgtgtttg 7620 tgggtatgca gatcatgaag
aactttgaac gattcgaggt caagaccttc tccctcctgc 7680 acaacttctg
tctggtctcc atctccgcct acatgtgcgg tggcatcctg tacgaggctt 7740
atcaggccaa ctatggactg tttgagaacg ctgccgatca caccttcaag ggtctcccta
7800 tggctaagat gatctggctc ttctacttct ccaagatcat ggagtttgtc
gacaccatga 7860 tcatggtcct caagaagaac aaccgacaga tttcctttct
gcacgtgtac caccactctt 7920 ccatcttcac catctggtgg ctggtcacct
tcgttgctcc caacggtgaa gcctacttct 7980 ctgctgccct gaactccttc
atccacgtca tcatgtacgg ctactacttt ctgtctgccc 8040 tgggcttcaa
gcaggtgtcg ttcatcaagt tctacatcac tcgatcccag atgacccagt 8100
tctgcatgat gtctgtccag tcttcctggg acatgtacgc catgaaggtc cttggccgac
8160 ctggataccc cttcttcatc accgctctgc tctggttcta catgtggacc
atgctcggtc 8220 tcttctacaa cttttaccga aagaacgcca agctcgccaa
gcaggccaag gctgacgctg 8280 ccaaggagaa ggccagaaag ctccagtaag
cggccgcaag tgtggatggg gaagtgagtg 8340 cccggttctg tgtgcacaat
tggcaatcca agatggatgg attcaacaca gggatatagc 8400 gagctacgtg
gtggtgcgag gatatagcaa cggatattta tgtttgacac ttgagaatgt 8460
acgatacaag cactgtccaa gtacaatact aaacatactg tacatactca tactcgtacc
8520 cgggcaacgg tttcacttga gtgcagtggc tagtgctctt actcgtacag
tgtgcaatac 8580 tgcgtatcat agtctttgat gtatatcgta ttcattcatg
ttagttgcgt acgaagtcgt 8640 caatgatgtc gatatgggtt ttgatcatgc
acacataagg tccgacctta tcggcaagct 8700 caatgagctc cttggtggtg
gtaacatcca gagaagcaca caggttggtt ttcttggctg 8760 ccacgagctt
gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt 8820
aggagggcat tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta
8880 tcggaacctt atctggggca gtgaagtata tgttatggta atagttacga
gttagttgaa 8940 cttatagata gactggacta tacggctatc ggtccaaatt
agaaagaacg tcaatggctc 9000 tctgggcgtc gcctttgccg acaaaaatgt
gatcatgatg aaagccagca atgacgttgc 9060 agctgatatt gttgtcggcc
aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca 9120 acgaagaatg
tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag 9180
gcggcaatga cgagtcagac agatactcgt cgaccttttc cttgggaacc accaccgtca
9240 gcccttctga ctcacgtatt gtagccaccg acacaggcaa cagtccgtgg
atagcagaat 9300 atgtcttgtc ggtccatttc tcaccaactt taggcgtcaa
gtgaatgttg cagaagaagt 9360 atgtgccttc attgagaatc ggtgttgctg
atttcaataa agtcttgaga tcagtttggc 9420 gcgccagctg cattaatgaa
tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 9480 ctcttccgct
tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 9540
atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
9600 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc 9660 gtttttccat aggctccgcc cccctgacga gcatcacaaa
aatcgacgct caagtcagag 9720 gtggcgaaac ccgacaggac tataaagata
ccaggcgttt ccccctggaa gctccctcgt 9780 gcgctctcct gttccgaccc
tgccgcttac cggatacctg tccgcctttc tcccttcggg 9840 aagcgtggcg
ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 9900
ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
9960 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac 10020 tggtaacagg attagcagag cgaggtatgt aggcggtgct
acagagttct tgaagtggtg 10080 gcctaactac ggctacacta gaagaacagt
atttggtatc tgcgctctgc tgaagccagt 10140 taccttcgga aaaagagttg
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 10200 tggttttttt
gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 10260
tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
10320 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt 10380 taaatcaatc taaagtatat atgagtaaac ttggtctgac
agttaccaat gcttaatcag 10440 tgaggcacct atctcagcga tctgtctatt
tcgttcatcc atagttgcct gactccccgt 10500 cgtgtagata actacgatac
gggagggctt accatctggc cccagtgctg caatgatacc 10560 gcgagaccca
cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 10620
cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
10680 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctac 10740 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
ttcagctccg gttcccaacg 10800 atcaaggcga gttacatgat cccccatgtt
gtgcaaaaaa gcggttagct ccttcggtcc 10860 tccgatcgtt gtcagaagta
agttggccgc agtgttatca ctcatggtta tggcagcact 10920 gcataattct
cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 10980
aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
11040 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc 11100 ttcggggcga aaactctcaa ggatcttacc gctgttgaga
tccagttcga tgtaacccac 11160 tcgtgcaccc aactgatctt cagcatcttt
tactttcacc agcgtttctg ggtgagcaaa 11220 aacaggaagg caaaatgccg
caaaaaaggg aataagggcg acacggaaat gttgaatact 11280 catactcttc
ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 11340
atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
11400 aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga
aaataccgca 11460 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
ttaaattttt gttaaatcag 11520 ctcatttttt aaccaatagg ccgaaatcgg
caaaatccct tataaatcaa aagaatagac 11580 cgagataggg ttgagtgttg
ttccagtttg gaacaagagt ccactattaa agaacgtgga 11640 ctccaacgtc
aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 11700
accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
11760 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
aggaagggaa 11820 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
gcggtcacgc tgcgcgtaac 11880 caccacaccc gccgcgctta atgcgccgct
acagggcgcg tccattcgcc attcaggctg 11940 cgcaactgtt gggaagggcg
atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 12000 gggggatgtg
ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 12060
tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggcccgacg
12120 tcgcatgcag tggtggtatt gtgactgggg atgtagttga gaataagtca
tacacaagtc 12180 agctttcttc gagcctcata taagtataag tagttcaacg
tattagcact gtacccagca 12240 tctccgtatc gagaaacaca acaacatgcc
ccattggaca gatcatgcgg atacacaggt 12300 tgtgcagtat catacatact
cgatcagaca ggtcgtctga ccatcataca agctgaacaa 12360 gcgctccata
cttgcacgct ctctatatac acagttaaat tacatatcca tagtctaacc 12420
tctaacagtt aatcttctgg taagcctccc agccagcctt ctggtatcgc ttggcctcct
12480 caataggatc tcggttctgg ccgtacagac ctcggccgac aattatgata
tccgttccgg 12540 tagacatgac atcctcaaca gttcggtact gctgtccgag
agcgtctccc ttgtcgtcaa 12600 gacccacccc gggggtcaga ataagccagt
cctcagagtc gcccttaat 12649 <210> SEQ ID NO 57 <211>
LENGTH: 819 <212> TYPE: DNA <213> ORGANISM:
Thraustochytrium aureum <220> FEATURE: <221> NAME/KEY:
misc_feature <223> OTHER INFORMATION: synthetic elongase
(codon-optimized) <400> SEQUENCE: 57 atggccaact cctctgtctg
ggacgacgtg gtcggacgag tcgagaccgg tgtcgaccag 60 tggatggacg
gagctaagcc ctacgctctg accgacggtc tgcccatgat ggacgtctcc 120
accatgctcg ccttcgaggt cggctacatg gccatgctgc tcttcggcat tcccatcatg
180 aagcagatgg agaagccctt cgagctgaag accatcaagc tgctccacaa
cctgttcctc 240 ttcggactgt ccctctacat gtgcgtcgag accatccgac
aggctatcct gggtggctac 300 aaggtcttcg gcaacgacat ggagaagggc
aacgagtccc acgctcaggg catgtcccga 360 atcgtctacg tgttctacgt
ctccaaggcc tacgagttcc tggacaccgc tatcatgatc 420 ctgtgcaaga
agttcaacca ggtctccttc ctgcacgtgt accaccatgc caccatcttc 480
gccatctggt gggctattgc caagtacgct cctggtggcg acgcctactt ctccgtcatc
540 ctcaactcct tcgtccacac cgtcatgtac gcctactact tcttttcctc
tcagggcttc 600 ggcttcgtca agcccatcaa gccctacatc accactctgc
agatgaccca gttcatggct 660 atgctggtgc agtccctgta cgactacctc
ttcccctgcg actaccctca ggctctggtc 720 cagctgctcg gcgtgtacat
gatcaccctg ctcgctctgt tcggcaactt ctttgtccag 780 tcctacctga
agaagcccaa gaagtccaag accaactaa 819 <210> SEQ ID NO 58
<211> LENGTH: 272 <212> TYPE: PRT <213> ORGANISM:
Thraustochytrium aureum <400> SEQUENCE: 58 Met Ala Asn Ser
Ser Val Trp Asp Asp Val Val Gly Arg Val Glu Thr 1 5 10 15 Gly Val
Asp Gln Trp Met Asp Gly Ala Lys Pro Tyr Ala Leu Thr Asp 20 25 30
Gly Leu Pro Met Met Asp Val Ser Thr Met Leu Ala Phe Glu Val Gly 35
40 45 Tyr Met Ala Met Leu Leu Phe Gly Ile Pro Ile Met Lys Gln Met
Glu 50 55 60 Lys Pro Phe Glu Leu Lys Thr Ile Lys Leu Leu His Asn
Leu Phe Leu 65 70 75 80 Phe Gly Leu Ser Leu Tyr Met Cys Val Glu Thr
Ile Arg Gln Ala Ile 85 90 95 Leu Gly Gly Tyr Lys Val Phe Gly Asn
Asp Met Glu Lys Gly Asn Glu 100 105 110 Ser His Ala Gln Gly Met Ser
Arg Ile Val Tyr Val Phe Tyr Val Ser 115 120 125 Lys Ala Tyr Glu Phe
Leu Asp Thr Ala Ile Met Ile Leu Cys Lys Lys 130 135 140 Phe Asn Gln
Val Ser Phe Leu His Val Tyr His His Ala Thr Ile Phe 145 150 155 160
Ala Ile Trp Trp Ala Ile Ala Lys Tyr Ala Pro Gly Gly Asp Ala Tyr 165
170 175 Phe Ser Val Ile Leu Asn Ser Phe Val His Thr Val Met Tyr Ala
Tyr 180 185 190 Tyr Phe Phe Ser Ser Gln Gly Phe Gly Phe Val Lys Pro
Ile Lys Pro 195 200 205 Tyr Ile Thr Thr Leu Gln Met Thr Gln Phe Met
Ala Met Leu Val Gln 210 215 220 Ser Leu Tyr Asp Tyr Leu Phe Pro Cys
Asp Tyr Pro Gln Ala Leu Val 225 230 235 240 Gln Leu Leu Gly Val Tyr
Met Ile Thr Leu Leu Ala Leu Phe Gly Asn 245 250 255 Phe Phe Val Gln
Ser Tyr Leu Lys Lys Pro Lys Lys Ser Lys Thr Asn 260 265 270
<210> SEQ ID NO 59 <211> LENGTH: 13034 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pDMW271 <400>
SEQUENCE: 59 cgatgcagaa ttcaggagag accgggttgg cggcgtattt gtgtcccaaa
aaacagcccc 60 aattgcccca attgacccca aattgaccca gtagcgggcc
caaccccggc gagagccccc 120 ttcaccccac atatcaaacc tcccccggtt
cccacacttg ccgttaaggg cgtagggtac 180 tgcagtctgg aatctacgct
tgttcagact ttgtactagt ttctttgtct ggccatccgg 240 gtaacccatg
ccggacgcaa aatagactac tgaaaatttt tttgctttgt ggttgggact 300
ttagccaagg gtataaaaga ccaccgtccc cgaattacct ttcctcttct tttctctctc
360 tccttgtcaa ctcacacccg aaatcgttaa gcatttcctt ctgagtataa
gaatcattca 420 ccatggatgg ctcccgaccc tgtcgctgcc gagaccgctg
cccagggtcc cactccccga 480 tacttcacct gggacgaggt cgcccagcga
tccggttgcg aggaacgatg gctggtcatc 540 gaccgaaagg tgtacaacat
ctctgagttc acccgacgac atcccggtgg ctcccgagtg 600 atctcgcact
acgctggaca ggacgccact gaccccttcg ttgcctttca cattaacaag 660
ggcctggtta agaagtacat gaactccctg ctcattggag agctgtctcc cgaacagcct
720 tcgtttgagc ctaccaagaa caaggagctg accgacgagt ttcgagagct
ccgagccacc 780 gttgagcgaa tgggactgat gaaggccaac catgtcttct
ttctgctcta cctgctccac 840 attcttctcc ttgacggagc tgcctggctt
accctgtggg tcttcggcac ttcctttctg 900 ccctttcttc tctgcgccgt
cctgctctct gccgtgcagg ctcaggctgg ttggcttcag 960 catgactttg
gtcacctttc cgtgttctct acctccaagt ggaaccacct gctccatcac 1020
ttcgtgatcg gccacctcaa gggtgctcct gcctcgtggt ggaaccacat gcatttccag
1080 caccatgcca agcccaactg ttttcgaaag gatcccgaca tcaacatgca
ccccttcttt 1140 ttcgctcttg gcaagatcct gtccgtcgag ctcggaaagc
agaagaagaa gtacatgccc 1200 tacaaccacc agcacaagta cttcttcctg
attggacctc ccgctctcct gcctctttac 1260 tttcagtggt acatctttta
ctttgttatt cagcgaaaga agtgggttga tcttgcctgg 1320 atgatcacct
tctacgtccg attcttcctg acctacgtcc ctctccttgg actgaaggcc 1380
tttctcggtc tgttctttat cgtccgattc ctggagtcca actggttcgt gtgggtgacc
1440 cagatgaacc acattcccat gcacattgac catgatcgaa acatggactg
ggtgtcgact 1500 cagctgcagg ccacctgcaa cgttcacaag tctgctttca
acgactggtt ttccggtcac 1560 ctcaactttc agattgagca ccatctgttt
cccaccatgc ctcgacacaa ctaccacaag 1620 gttgctcccc tggtccagtc
gctctgtgcc aagcatggca tcgagtacca gtccaagccc 1680 ctgctctctg
ccttcgctga catcattcac tcgctgaagg aatctggcca gctctggctc 1740
gatgcctacc tgcaccagta agcggccgca ttgatgattg gaaacacaca catgggttat
1800 atctaggtga gagttagttg gacagttata tattaaatca gctatgccaa
cggtaacttc 1860 attcatgtca acgaggaacc agtgactgca agtaatatag
aatttgacca ccttgccatt 1920 ctcttgcact cctttactat atctcattta
tttcttatat acaaatcact tcttcttccc 1980 agcatcgagc tcggaaacct
catgagcaat aacatcgtgg atctcgtcaa tagagggctt 2040 tttggactcc
ttgctgttgg ccaccttgtc cttgctgtct ggctcattct gtttcaacgc 2100
cttttaatta acggagtagg tctcggtgtc ggaagcgacg ccagatccgt catcctcctt
2160 tcgctctcca aagtagatac ctccgacgag ctctcggaca atgatgaagt
cggtgccctc 2220 aacgtttcgg atgggggaga gatcggcgag cttgggcgac
agcagctggc agggtcgcag 2280 gttggcgtac aggttcaggt cctttcgcag
cttgaggaga ccctgctcgg gtcgcacgtc 2340 ggttcgtccg tcgggagtgg
tccatacggt gttggcagcg cctccgacag caccgagcat 2400 aatagagtca
gcctttcggc agatgtcgag agtagcgtcg gtgatgggct cgccctcctt 2460
ctcaatggca gctcctccaa tgagtcggtc ctcaaacaca aactcggtgc cggaggcctc
2520 agcaacagac ttgagcacct tgacggcctc ggcaatcacc tcggggccac
agaagtcgcc 2580 gccgagaaga acaatcttct tggagtcagt cttggtcttc
ttagtttcgg gttccattgt 2640 ggatgtgtgt ggttgtatgt gtgatgtggt
gtgtggagtg aaaatctgtg gctggcaaac 2700 gctcttgtat atatacgcac
ttttgcccgt gctatgtgga agactaaacc tccgaagatt 2760 gtgactcagg
tagtgcggta tcggctaggg acccaaacct tgtcgatgcc gatagcatgc 2820
gacgtcgggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
2880 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
agcacatccc 2940 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
atcgcccttc ccaacagttg 3000 cgcagcctga atggcgaatg gacgcgccct
gtagcggcgc attaagcgcg gcgggtgtgg 3060 tggttacgcg cagcgtgacc
gctacacttg ccagcgccct agcgcccgct cctttcgctt 3120 tcttcccttc
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3180
tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
3240 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
ttgacgttgg 3300 agtccacgtt ctttaatagt ggactcttgt tccaaactgg
aacaacactc aaccctatct 3360 cggtctattc ttttgattta taagggattt
tgccgatttc ggcctattgg ttaaaaaatg 3420 agctgattta acaaaaattt
aacgcgaatt ttaacaaaat attaacgctt acaatttcct 3480 gatgcggtat
tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt 3540
ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
3600 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
aggaagagta 3660 tgagtattca acatttccgt gtcgccctta ttcccttttt
tgcggcattt tgccttcctg 3720 tttttgctca cccagaaacg ctggtgaaag
taaaagatgc tgaagatcag ttgggtgcac 3780 gagtgggtta catcgaactg
gatctcaaca gcggtaagat ccttgagagt tttcgccccg 3840 aagaacgttt
tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 3900
gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
3960 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
agagaattat 4020 gcagtgctgc cataaccatg agtgataaca ctgcggccaa
cttacttctg acaacgatcg 4080 gaggaccgaa ggagctaacc gcttttttgc
acaacatggg ggatcatgta actcgccttg 4140 atcgttggga accggagctg
aatgaagcca taccaaacga cgagcgtgac accacgatgc 4200 ctgtagcaat
ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 4260
cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
4320 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
cgtgggtctc 4380 gcggtatcat tgcagcactg gggccagatg gtaagccctc
ccgtatcgta gttatctaca 4440 cgacggggag tcaggcaact atggatgaac
gaaatagaca gatcgctgag ataggtgcct 4500 cactgattaa gcattggtaa
ctgtcagacc aagtttactc atatatactt tagattgatt 4560 taaaacttca
tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 4620
ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
4680 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
acaaaaaaac 4740 caccgctacc agcggtggtt tgtttgccgg atcaagagct
accaactctt tttccgaagg 4800 taactggctt cagcagagcg cagataccaa
atactgttct tctagtgtag ccgtagttag 4860 gccaccactt caagaactct
gtagcaccgc ctacatacct cgctctgcta atcctgttac 4920 cagtggctgc
tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4980
taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
5040 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
agcgccacgc 5100 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
cagggtcgga acaggagagc 5160 gcacgaggga gcttccaggg ggaaacgcct
ggtatcttta tagtcctgtc gggtttcgcc 5220 acctctgact tgagcgtcga
tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 5280 acgccagcaa
cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 5340
tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
5400 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
gaagcggaag 5460 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
gattcattaa tgcagctggc 5520 gcgcccactg agctcgtcta acggacttga
tatacaacca attaaaacaa atgaaaagaa 5580 atacagttct ttgtatcatt
tgtaacaatt accctgtaca aactaaggta ttgaaatccc 5640 acaatattcc
caaagtccac ccctttccaa attgtcatgc ctacaactca tataccaagc 5700
actaacctac caaacaccac taaaacccca caaaatatat cttaccgaat atacagtaac
5760 aagctaccac cacactcgtt gggtgcagtc gccagcttaa agatatctat
ccacatcagc 5820 cacaactccc ttcctttaat aaaccgacta cacccttggc
tattgaggtt atgagtgaat 5880 atactgtaga caagacactt tcaagaagac
tgtttccaaa acgtaccact gtcctccact 5940 acaaacacac ccaatctgct
tcttctagtc aaggttgcta caccggtaaa ttataaatca 6000 tcatttcatt
agcagggcag ggcccttttt atagagtctt atacactagc ggaccctgcc 6060
ggtagaccaa cccgcaggcg cgtcagtttg ctccttccat caatgcgtcg tagaaacgac
6120 ttactccttc ttgagcagct ccttgacctt gttggcaaca agtctccgac
ctcggaggtg 6180 gaggaagagc ctccgatatc ggcggtagtg ataccagcct
cgacggactc cttgacggca 6240 gcctcaacag cgtcaccggc gggcttcatg
ttaagagaga acttgagcat catggcggca 6300 gacagaatgg tggcgtacgc
aactaacatg aatgaatacg atatacatca aagactatga 6360 tacgcagtat
tgcacactgt acgagtaaga gcactagcca ctgcactcaa gtgaaaccgt 6420
tgcccgggta cgagtatgag tatgtacagt atgtttagta ttgtacttgg acagtgcttg
6480 tatcgtacat tctcaagtgt caaacataaa tatccgttgc tatatcctcg
caccaccacg 6540 tagctcgcta tatccctgtg ttgaatccat ccatcttgga
ttgccaattg tgcacacaga 6600 accgggcact cacttcccca tccacacttg
cggccgctta gctgcctact cttccttggg 6660 acggagtcca agaacacgca
agtgctccaa atgtgaagca aatgcttgcc aaaacgtatc 6720 cttgacaagg
tatggaacct tgtactcgct gcaggtgttc ttgatgatgg ccagaatatc 6780
gggataatgg tgctgcgaca cgttggggaa cagatggtgc acagcctggt agttcaagct
6840 gccagtgatg ctggtccaga ggtgcgaatc gtgtgcgtaa tcctgcgtag
tctcgacctg 6900 catagctgcc cagtcctttt ggatgatccc gttctcgtca
ggcaacggcc actgaacttc 6960 ctcaacaacg tggttcgcct ggaaggtcag
cgccagccag taagacgaca ccatgtccgc 7020 gaccgtgaac aagagcagca
ccttgcccag gggcagatac tgcaggggaa caatcaggcg 7080 ataccagaca
aagaaagcct tgccgcccca gaacatcaca gtgtgccatg tcgagatggg 7140
attgacacga atagcgtcat tggtcttgac aaagtacaaa atgttgatgt cctgaatgcg
7200 caccttgaac gccagcagtc cgtacaggaa aggaacaaac atgtgctggt
tgatgtggtt 7260 gacaaaccac ttttggttgg gcttgatacg acgaacatcg
ggctcagacg tcgacacgtc 7320 gggatctgct ccagcaatgt tggtgtaggg
gtgatggccg agcatatgtt ggtacatcca 7380 caccaggtac gatgctccgt
tgaaaaagtc gtgcgtggct cccagaatct tccagacagt 7440 ggggttgtgg
gtcactgaaa agtgagacgc atcatgaaga gggttgagtc cgacttgtgc 7500
gcacgcaaat cccatgatga ttgcaaacac cacctgaagc catgtgcgtt cgacaacgaa
7560 aggcacaaag agctgcgcgt agtaggaagc gatcaaggat ccaaagataa
gagcgtatcg 7620 tccccagatc tctggtctat tcttgggatc aatgttccga
tccgtaaagt agccctcgac 7680 tctcgtcttg atggttttgt ggaacaccgt
tggctccggg aagatgggca gctcattcga 7740 gaccagtgta ccgacatagt
acttcttcat aatggcatct gcagccccaa acgcgtgata 7800 catctcaaag
accggagtaa catctcggcc agctccgagc aggagagtgt ccactccacc 7860
aggatggcgg ctcaagaact ttgtgacatc gtacaccctg ccgcggatgg ccaagagtag
7920 gtcgtccttg gtgttatggg ccgccagctc ttcccaggtg aaggtttttc
cttggtccgt 7980 tcccatggag agctgggtta gtttgtgtag agagtgtgtg
ttgctagcga ctttcggatt 8040 gtgtcattac acaaaacgcg tcgtctcgac
actgatcttg tcgtggatac tcacggctcg 8100 gacatcgtcg ccgacgatga
caccggactt tcgcttaagg acgtcagtaa caggcattgt 8160 gtgatgtgta
gtttagattt cgaatctgtg gggaaagaaa ggaaaaaaga gactggcaac 8220
cgattgggag agccactgtt tatatatacc ctagacaagc cccccgcttg taagatgttg
8280 gtcaatgtaa accagtatta aggttggcaa gtgcaggaga agcaaggtgt
gggtaccgag 8340 caatggaaat gtgcggaagg caaaaaaatg aggccacggc
ctattgtcgg ggctatatcc 8400 agggggcgat tgaagtacac taacatgaca
tgtgtccaca gaccctcaat ctggcctgat 8460 gagccaaatc catacgcgct
ttcgcagctc taaaggctat aacaagtcac accaccctgc 8520 tcgacctcag
cgccctcact ttttgttaag acaaactgta cacgctgttc cagcgttttc 8580
tgcctgcacc tggtgggaca tttggtgcaa cctaaagtgc tcggaacctc tgtggtgtcc
8640 agatcagcgc agcagttccg aggtagtttt gaggccctta gatgatgcaa
tggtgtcagt 8700 cgctggatca cgagtcttaa tggcagtatt cgttcttatt
tgtgccattg agccccgtta 8760 tcctcgtatc ttctaccccc catcccatcc
ctttgttggt gcaaccctac ccatttattg 8820 ttgggtgcag cccaaccgac
gtggagagct tggcttggcc atataaaaag gcccccccct 8880 agtggcaatg
gcagaaagtc agctgtgagt tgttgaattt gtcatctagg cggcctggcc 8940
gtcttctccg gggcaattta aattccttca cttcaagttc attcttcatc tgcttctgtt
9000 ttactttgac aggcaaatga agacatggta cgacttgatg gaggccaaga
acgccatttc 9060 accccgagac accgaagtgc ctgaaatcct ggctgccccc
attgataaca tcggaaacta 9120 cggtattccg gaaagtgtat atagaacctt
tccccagctt gtgtctgtgg atatggatgg 9180 tgtaatcccc tttgagtact
cgtcttggct tctctccgag cagtatgagg ctctctaatc 9240 tagcgcattt
aatatctcaa tgtatttata tatttatctt ctcatgcggc cgcttagctg 9300
cctactcttc cttgggacgg agtccaagaa cacgcaagtg ctccaaatgt gaagcaaatg
9360 cttgccaaaa cgtatccttg acaaggtatg gaaccttgta ctcgctgcag
gtgttcttga 9420 tgatggccag aatatcggga taatggtgct gcgacacgtt
ggggaacaga tggtgcacag 9480 cctggtagtt caagctgcca gtgatgctgg
tccagaggtg cgaatcgtgt gcgtaatcct 9540 gcgtagtctc gacctgcata
gctgcccagt ccttttggat gatcccgttc tcgtcaggca 9600 acggccactg
aacttcctca acaacgtggt tcgcctggaa ggtcagcgcc agccagtaag 9660
acgacaccat gtccgcgacc gtgaacaaga gcagcacctt gcccaggggc agatactgca
9720 ggggaacaat caggcgatac cagacaaaga aagccttgcc gccccagaac
atcacagtgt 9780 gccatgtcga gatgggattg acacgaatag cgtcattggt
cttgacaaag tacaaaatgt 9840 tgatgtcctg aatgcgcacc ttgaacgcca
gcagtccgta caggaaagga acaaacatgt 9900 gctggttgat gtggttgaca
aaccactttt ggttgggctt gatacgacga acatcgggct 9960 cagacgtcga
cacgtcggga tctgctccag caatgttggt gtaggggtga tggccgagca 10020
tatgttggta catccacacc aggtacgatg ctccgttgaa aaagtcgtgc gtggctccca
10080 gaatcttcca gacagtgggg ttgtgggtca ctgaaaagtg agacgcatca
tgaagagggt 10140 tgagtccgac ttgtgcgcac gcaaatccca tgatgattgc
aaacaccacc tgaagccatg 10200 tgcgttcgac aacgaaaggc acaaagagct
gcgcgtagta ggaagcgatc aaggatccaa 10260 agataagagc gtatcgtccc
cagatctctg gtctattctt gggatcaatg ttccgatccg 10320 taaagtagcc
ctcgactctc gtcttgatgg ttttgtggaa caccgttggc tccgggaaga 10380
tgggcagctc attcgagacc agtgtaccga catagtactt cttcataatg gcatctgcag
10440 ccccaaacgc gtgatacatc tcaaagaccg gagtaacatc tcggccagct
ccgagcagga 10500 gagtgtccac tccaccagga tggcggctca agaactttgt
gacatcgtac accctgccgc 10560 ggatggccaa gagtaggtcg tccttggtgt
tatgggccgc cagctcttcc caggtgaagg 10620 tttttccttg gtccgttccc
atggtgaatg attcttatac tcagaaggaa atgcttaacg 10680 atttcgggtg
tgagttgaca aggagagaga gaaaagaaga ggaaaggtaa ttcggggacg 10740
gtggtctttt atacccttgg ctaaagtccc aaccacaaag caaaaaaatt ttcagtagtc
10800 tattttgcgt ccggcatggg ttacccggat ggccagacaa agaaactagt
acaaagtctg 10860 aacaagcgta gattccagac tgcagtaccc tacgccctta
acggcaagtg tgggaaccgg 10920 gggaggtttg atatgtgggg tgaagggggc
tctcgccggg gttgggcccg ctactgggtc 10980 aatttggggt caattggggc
aattggggct gttttttggg acacaaatac gccgccaacc 11040 cggtctctcc
tgatcgatgg gctgcaggaa ttctacaata cgtgagtcag aagggctgac 11100
ggtggtggtt cccaaggaaa aggtcgacga gtatctgtct gactcgtcat tgccgccttt
11160 ggagtacgac tccaactatg agtgtgcttg gatcactttg acgatacatt
cttcgttgga 11220 ggctgtgggt ctgacagctg cgttttcggc gcggttggcc
gacaacaata tcagctgcaa 11280 cgtcattgct ggctttcatc atgatcacat
ttttgtcggc aaaggcgacg cccagagagc 11340 cattgacgtt ctttctaatt
tggaccgata gccgtatagt ccagtctatc tataagttca 11400 actaactcgt
aactattacc ataacatata cttcactgcc ccagataagg ttccgataaa 11460
aagttctgca gactaaattt atttcagtct cctcttcacc accaaaatgc cctcctacga
11520 agctcgagct aacgtccaca agtccgcctt tgccgctcga gtgctcaagc
tcgtggcagc 11580 caagaaaacc aacctgtgtg cttctctgga tgttaccacc
accaaggagc tcattgagct 11640 tgccgataag gtcggacctt atgtgtgcat
gatcaaaacc catatcgaca tcattgacga 11700 cttcacctac gccggcactg
tgctccccct caaggaactt gctcttaagc acggtttctt 11760 cctgttcgag
gacagaaagt tcgcagatat tggcaacact gtcaagcacc agtaccggtg 11820
tcaccgaatc gccgagtggt ccgatatcac caacgcccac ggtgtacccg gaaccggaat
11880 cattgctggc ctgcgagctg gtgccgagga aactgtctct gaacagaaga
aggaggacgt 11940 ctctgactac gagaactccc agtacaagga gttcctagtc
ccctctccca acgagaagct 12000 ggccagaggt ctgctcatgc tggccgagct
gtcttgcaag ggctctctgg ccactggcga 12060 gtactccaag cagaccattg
agcttgcccg atccgacccc gagtttgtgg ttggcttcat 12120 tgcccagaac
cgacctaagg gcgactctga ggactggctt attctgaccc ccggggtggg 12180
tcttgacgac aagggagacg ctctcggaca gcagtaccga actgttgagg atgtcatgtc
12240 taccggaacg gatatcataa ttgtcggccg aggtctgtac ggccagaacc
gagatcctat 12300 tgaggaggcc aagcgatacc agaaggctgg ctgggaggct
taccagaaga ttaactgtta 12360 gaggttagac tatggatatg taatttaact
gtgtatatag agagcgtgca agtatggagc 12420 gcttgttcag cttgtatgat
ggtcagacga cctgtctgat cgagtatgta tgatactgca 12480 caacctgtgt
atccgcatga tctgtccaat ggggcatgtt gttgtgtttc tcgatacgga 12540
gatgctgggt acagtgctaa tacgttgaac tacttatact tatatgaggc tcgaagaaag
12600 ctgacttgtg tatgacttat tctcaactac atccccagtc acaataccac
cactgcacta 12660 ccactacacc agatctgcgt acactgttta aacggtaggt
tagtgcttgg tatatgagtt 12720 gtaggcatga caatttggaa aggggtggac
tttgggaata ttgtgggatt tcaatacctt 12780 agtttgtaca gggtaattgt
tacaaatgat acaaagaact gtatttcttt tcatttgttt 12840 taattggttg
tatatcaagt ccgttagacg agctcagtgc cttggctttt ggcactgtat 12900
ttcattttta gaggtacact acattcagtg aggtatggta aggttgaggg cataatgaag
12960 gcaccttgta ctgacagtca cagacctctc accgagaatt ttatgagata
tactcgggtt 13020 cattttaggc tcat 13034 <210> SEQ ID NO 60
<211> LENGTH: 1335 <212> TYPE: DNA <213>
ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY:
misc_feature <223> OTHER INFORMATION: synthetic delta-5
desaturase (codon-optimized) <400> SEQUENCE: 60 atggctcccg
accctgtcgc tgccgagacc gctgcccagg gtcccactcc ccgatacttc 60
acctgggacg aggtcgccca gcgatccggt tgcgaggaac gatggctggt catcgaccga
120 aaggtgtaca acatctctga gttcacccga cgacatcccg gtggctcccg
agtgatctcg 180 cactacgctg gacaggacgc cactgacccc ttcgttgcct
ttcacattaa caagggcctg 240 gttaagaagt acatgaactc cctgctcatt
ggagagctgt ctcccgaaca gccttcgttt 300 gagcctacca agaacaagga
gctgaccgac gagtttcgag agctccgagc caccgttgag 360 cgaatgggac
tgatgaaggc caaccatgtc ttctttctgc tctacctgct ccacattctt 420
ctccttgacg gagctgcctg gcttaccctg tgggtcttcg gcacttcctt tctgcccttt
480 cttctctgcg ccgtcctgct ctctgccgtg caggctcagg ctggttggct
tcagcatgac 540 tttggtcacc tttccgtgtt ctctacctcc aagtggaacc
acctgctcca tcacttcgtg 600 atcggccacc tcaagggtgc tcctgcctcg
tggtggaacc acatgcattt ccagcaccat 660 gccaagccca actgttttcg
aaaggatccc gacatcaaca tgcacccctt ctttttcgct 720 cttggcaaga
tcctgtccgt cgagctcgga aagcagaaga agaagtacat gccctacaac 780
caccagcaca agtacttctt cctgattgga cctcccgctc tcctgcctct ttactttcag
840 tggtacatct tttactttgt tattcagcga aagaagtggg ttgatcttgc
ctggatgatc 900 accttctacg tccgattctt cctgacctac gtccctctcc
ttggactgaa ggcctttctc 960 ggtctgttct ttatcgtccg attcctggag
tccaactggt tcgtgtgggt gacccagatg 1020 aaccacattc ccatgcacat
tgaccatgat cgaaacatgg actgggtgtc gactcagctg 1080 caggccacct
gcaacgttca caagtctgct ttcaacgact ggttttccgg tcacctcaac 1140
tttcagattg agcaccatct gtttcccacc atgcctcgac acaactacca caaggttgct
1200 cccctggtcc agtcgctctg tgccaagcat ggcatcgagt accagtccaa
gcccctgctc 1260 tctgccttcg ctgacatcat tcactcgctg aaggaatctg
gccagctctg gctcgatgcc 1320 tacctgcacc agtaa 1335 <210> SEQ ID
NO 61 <211> LENGTH: 444 <212> TYPE: PRT <213>
ORGANISM: Homo sapiens <400> SEQUENCE: 61 Met Ala Pro Asp Pro
Val Ala Ala Glu Thr Ala Ala Gln Gly Pro Thr 1 5 10 15 Pro Arg Tyr
Phe Thr Trp Asp Glu Val Ala Gln Arg Ser Gly Cys Glu 20 25 30 Glu
Arg Trp Leu Val Ile Asp Arg Lys Val Tyr Asn Ile Ser Glu Phe 35 40
45 Thr Arg Arg His Pro Gly Gly Ser Arg Val Ile Ser His Tyr Ala Gly
50 55 60 Gln Asp Ala Thr Asp Pro Phe Val Ala Phe His Ile Asn Lys
Gly Leu 65 70 75 80 Val Lys Lys Tyr Met Asn Ser Leu Leu Ile Gly Glu
Leu Ser Pro Glu 85 90 95 Gln Pro Ser Phe Glu Pro Thr Lys Asn Lys
Glu Leu Thr Asp Glu Phe 100 105 110 Arg Glu Leu Arg Ala Thr Val Glu
Arg Met Gly Leu Met Lys Ala Asn 115 120 125 His Val Phe Phe Leu Leu
Tyr Leu Leu His Ile Leu Leu Leu Asp Gly 130 135 140 Ala Ala Trp Leu
Thr Leu Trp Val Phe Gly Thr Ser Phe Leu Pro Phe 145 150 155 160 Leu
Leu Cys Ala Val Leu Leu Ser Ala Val Gln Ala Gln Ala Gly Trp 165 170
175 Leu Gln His Asp Phe Gly His Leu Ser Val Phe Ser Thr Ser Lys Trp
180 185 190 Asn His Leu Leu His His Phe Val Ile Gly His Leu Lys Gly
Ala Pro 195 200 205 Ala Ser Trp Trp Asn His Met His Phe Gln His His
Ala Lys Pro Asn 210 215 220 Cys Phe Arg Lys Asp Pro Asp Ile Asn Met
His Pro Phe Phe Phe Ala 225 230 235 240 Leu Gly Lys Ile Leu Ser Val
Glu Leu Gly Lys Gln Lys Lys Lys Tyr 245 250 255 Met Pro Tyr Asn His
Gln His Lys Tyr Phe Phe Leu Ile Gly Pro Pro 260 265 270 Ala Leu Leu
Pro Leu Tyr Phe Gln Trp Tyr Ile Phe Tyr Phe Val Ile 275 280 285 Gln
Arg Lys Lys Trp Val Asp Leu Ala Trp Met Ile Thr Phe Tyr Val 290 295
300 Arg Phe Phe Leu Thr Tyr Val Pro Leu Leu Gly Leu Lys Ala Phe Leu
305 310 315 320 Gly Leu Phe Phe Ile Val Arg Phe Leu Glu Ser Asn Trp
Phe Val Trp 325 330 335 Val Thr Gln Met Asn His Ile Pro Met His Ile
Asp His Asp Arg Asn 340 345 350 Met Asp Trp Val Ser Thr Gln Leu Gln
Ala Thr Cys Asn Val His Lys 355 360 365 Ser Ala Phe Asn Asp Trp Phe
Ser Gly His Leu Asn Phe Gln Ile Glu 370 375 380 His His Leu Phe Pro
Thr Met Pro Arg His Asn Tyr His Lys Val Ala 385 390 395 400 Pro Leu
Val Gln Ser Leu Cys Ala Lys His Gly Ile Glu Tyr Gln Ser 405 410 415
Lys Pro Leu Leu Ser Ala Phe Ala Asp Ile Ile His Ser Leu Lys Glu 420
425 430 Ser Gly Gln Leu Trp Leu Asp Ala Tyr Leu His Gln 435 440
<210> SEQ ID NO 62 <211> LENGTH: 3800 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pPaD17S <400>
SEQUENCE: 62 ggccgcatcg gatcccgggc ccgtcgactg cagaggcctg catgcaagct
tggcgtaatc 60 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
acaattccac acaacatacg 120 agccggaagc ataaagtgta aagcctgggg
tgcctaatga gtgagctaac tcacattaat 180 tgcgttgcgc tcactgcccg
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 240 aatcggccaa
cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 300
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
360 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
gagcaaaagg 420 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
gcgtttttcc ataggctccg 480 cccccctgac gagcatcaca aaaatcgacg
ctcaagtcag aggtggcgaa acccgacagg 540 actataaaga taccaggcgt
ttccccctgg aagctccctc gtgcgctctc ctgttccgac 600 cctgccgctt
accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 660
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
720 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
gtcttgagtc 780 caacccggta agacacgact tatcgccact ggcagcagcc
actggtaaca ggattagcag 840 agcgaggtat gtaggcggtg ctacagagtt
cttgaagtgg tggcctaact acggctacac 900 tagaagaaca gtatttggta
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 960 tggtagctct
tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1020
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
1080 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
gattatcaaa 1140 aaggatcttc acctagatcc ttttaaatta aaaatgaagt
tttaaatcaa tctaaagtat 1200 atatgagtaa acttggtctg acagttacca
atgcttaatc agtgaggcac ctatctcagc 1260 gatctgtcta tttcgttcat
ccatagttgc ctgactcccc gtcgtgtaga taactacgat 1320 acgggagggc
ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1380
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
1440 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
gagtaagtag 1500 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
acaggcatcg tggtgtcacg 1560 ctcgtcgttt ggtatggctt cattcagctc
cggttcccaa cgatcaaggc gagttacatg 1620 atcccccatg ttgtgcaaaa
aagcggttag ctccttcggt cctccgatcg ttgtcagaag 1680 taagttggcc
gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 1740
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
1800 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
ataccgcgcc 1860 acatagcaga actttaaaag tgctcatcat tggaaaacgt
tcttcggggc gaaaactctc 1920 aaggatctta ccgctgttga gatccagttc
gatgtaaccc actcgtgcac ccaactgatc 1980 ttcagcatct tttactttca
ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 2040 cgcaaaaaag
ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 2100
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
2160 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
cacctgacgt 2220 ctaagaaacc attattatca tgacattaac ctataaaaat
aggcgtatca cgaggccctt 2280 tcgtctcgcg cgtttcggtg atgacggtga
aaacctctga cacatgcagc tcccggagac 2340 ggtcacagct tgtctgtaag
cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 2400 gggtgttggc
gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 2460
agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
2520 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
gggcctcttc 2580 gctattacgc cagctggcga aagggggatg tgctgcaagg
cgattaagtt gggtaacgcc 2640 agggttttcc cagtcacgac gttgtaaaac
gacggccagt gaattcgagc tcggtacctc 2700 gcgaatgcat ctagatccat
ggcttcctct accgttgccg ctccctacga gttccctact 2760 ctcaccgaga
tcaagcgatc cctgcctgcc cactgcttcg aagcctctgt tccctggtcc 2820
ctctactata ccgtgcgagc tctgggcatt gccggttccc ttgctctcgg actgtactat
2880 gctcgagccc ttgctatcgt gcaggagttt gcactgctcg atgccgtcct
ttgcactggc 2940 tacattctgc tccagggtat cgtgttctgg ggattcttta
ccatcggtca cgactgtgga 3000 catggtgcct tctcgcgatc ccacctgctc
aacttctctg ttggcacact cattcactcc 3060 atcattctga ctccctacga
gtcgtggaag atcagccatc gacaccatca caagaacacc 3120 ggcaacatcg
acaaggatga gatcttctac cctcagcgag aagccgactc tcatcccctg 3180
tcccgacaca tggtcatctc ccttggttcg gcttggtttg cctacctcgt tgctggattt
3240 cctccccgaa aggtcaacca cttcaatccc tgggagcctc tctacctgcg
aagaatgtct 3300 gccgtcatca tttccctcgg ctctctcgtg gcctttgctg
gtctgtacgc ctaccttacc 3360 tacgtctacg gcctcaagac catggctctg
tattacttcg cacctctctt tggattcgcc 3420 accatgctgg ttgtcactac
cttcctccat cacaacgacg aggaaactcc ctggtacgcc 3480 gattcggagt
ggacctatgt caagggcaac ttgtcctctg tggaccgaag ctacggagcc 3540
ctcatcgaca acctgtccca caacattggt acacatcaga tccaccatct gtttcccatc
3600 attcctcact acaagctcaa cgaggccact gctgccttcg ctcaggcctt
tcccgaactg 3660 gtgcgaaagt cggcttctcc catcattccc accttcatcc
gaattggtct tatgtacgcc 3720 aagtacggcg tggtcgacaa ggatgccaag
atgtttaccc tcaaggaggc caaggctgcc 3780 aagaccaaag ccaactaagc 3800
<210> SEQ ID NO 63 <211> LENGTH: 14655 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pZKLeuN-29E3
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (8822)..(8822) <223> OTHER INFORMATION: n is a, c,
g, or t <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (8827)..(8830) <223> OTHER INFORMATION:
n is a, c, g, or t <400> SEQUENCE: 63 cgattgttgt ctactaacta
tcgtacgata acttcgtata gcatacatta tacgaagtta 60 tcgcgtcgac
gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta 120
tgagtgtgct tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc
180 tgcgttttcg gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg
ctggctttca 240 tcatgatcac atttttgtcg gcaaaggcga cgcccagaga
gccattgacg ttctttctaa 300 tttggaccga tagccgtata gtccagtcta
tctataagtt caactaactc gtaactatta 360 ccataacata tacttcactg
ccccagataa ggttccgata aaaagttctg cagactaaat 420 ttatttcagt
ctcctcttca ccaccaaaat gccctcctac gaagctcgag ctaacgtcca 480
caagtccgcc tttgccgctc gagtgctcaa gctcgtggca gccaagaaaa ccaacctgtg
540 tgcttctctg gatgttacca ccaccaagga gctcattgag cttgccgata
aggtcggacc 600 ttatgtgtgc atgatcaaaa cccatatcga catcattgac
gacttcacct acgccggcac 660 tgtgctcccc ctcaaggaac ttgctcttaa
gcacggtttc ttcctgttcg aggacagaaa 720 gttcgcagat attggcaaca
ctgtcaagca ccagtaccgg tgtcaccgaa tcgccgagtg 780 gtccgatatc
accaacgccc acggtgtacc cggaaccgga atcattgctg gcctgcgagc 840
tggtgccgag gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc
900 ccagtacaag gagttcctag tcccctctcc caacgagaag ctggccagag
gtctgctcat 960 gctggccgag ctgtcttgca agggctctct ggccactggc
gagtactcca agcagaccat 1020 tgagcttgcc cgatccgacc ccgagtttgt
ggttggcttc attgcccaga accgacctaa 1080 gggcgactct gaggactggc
ttattctgac ccccggggtg ggtcttgacg acaagggaga 1140 cgctctcgga
cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat 1200
aattgtcggc cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata
1260 ccagaaggct ggctgggagg cttaccagaa gattaactgt tagaggttag
actatggata 1320 tgtaatttaa ctgtgtatat agagagcgtg caagtatgga
gcgcttgttc agcttgtatg 1380 atggtcagac gacctgtctg atcgagtatg
tatgatactg cacaacctgt gtatccgcat 1440 gatctgtcca atggggcatg
ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct 1500 aatacgttga
actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt 1560
attctcaact acatccccag tcacaatacc accactgcac taccactaca ccaaaaccat
1620 gatcaaacca cccatggact tcctggaggc agaagaactt gttatggaaa
agctcaagag 1680 agagatcata acttcgtata gcatacatta tacgaagtta
tcctgcaggt aaaggaattc 1740 tggagtttct gagagaaaaa ggcaagatac
gtatgtaaca aagcgacgca tggtacaata 1800 ataccggagg catgtatcat
agagagttag tggttcgatg atggcactgg tgcctggtat 1860 gactttatac
ggctgactac atatttgtcc tcagacatac aattacagtc aagcacttac 1920
ccttggacat ctgtaggtac cccccggcca agacgatctc agcgtgtcgt atgtcggatt
1980 ggcgtagctc cctcgctcgt caattggctc ccatctactt tcttctgctt
ggctacaccc 2040 agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc
tcccagtatt accaactcta 2100 aatgacatga tgtgattggg tctacacttt
catatcagag ataaggagta gcacagttgc 2160 ataaaaagcc caactctaat
cagcttcttc ctttcttgta attagtacaa aggtgattag 2220 cgaaatctgg
aagcttagtt ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga 2280
aaaaccacag ttttgagaac agggaggtaa cgaaggatcg tatatatata tatatatata
2340 tatacccacg gatcccgaga ccggcctttg attcttccct acaaccaacc
attctcacca 2400 ccctaattca caaccatgga gtctggaccc atgcctgctg
gcattccctt ccctgagtac 2460 tatgacttct ttatggactg gaagactccc
ctggccatcg ctgccaccta cactgctgcc 2520 gtcggtctct tcaaccccaa
ggttggcaag gtctcccgag tggttgccaa gtcggctaac 2580 gcaaagcctg
ccgagcgaac ccagtccgga gctgccatga ctgccttcgt ctttgtgcac 2640
aacctcattc tgtgtgtcta ctctggcatc accttctact acatgtttcc tgctatggtc
2700 aagaacttcc gaacccacac actgcacgaa gcctactgcg acacggatca
gtccctctgg 2760 aacaacgcac ttggctactg gggttacctc ttctacctgt
ccaagttcta cgaggtcatt 2820 gacaccatca tcatcatcct gaagggacga
cggtcctcgc tgcttcagac ctaccaccat 2880 gctggagcca tgattaccat
gtggtctggc atcaactacc aagccactcc catttggatc 2940 tttgtggtct
tcaactcctt cattcacacc atcatgtact gttactatgc cttcacctct 3000
atcggattcc atcctcctgg caaaaagtac ctgacttcga tgcagattac tcagtttctg
3060 gtcggtatca ccattgccgt gtcctacctc ttcgttcctg gctgcatccg
aacacccggt 3120 gctcagatgg ctgtctggat caacgtcggc tacctgtttc
ccttgaccta tctgttcgtg 3180 gactttgcca agcgaaccta ctccaagcga
tctgccattg ccgctcagaa aaaggctcag 3240 taagcggccg cattgatgat
tggaaacaca cacatgggtt atatctaggt gagagttagt 3300 tggacagtta
tatattaaat cagctatgcc aacggtaact tcattcatgt caacgaggaa 3360
ccagtgactg caagtaatat agaatttgac caccttgcca ttctcttgca ctcctttact
3420 atatctcatt tatttcttat atacaaatca cttcttcttc ccagcatcga
gctcggaaac 3480 ctcatgagca ataacatcgt ggatctcgtc aatagagggc
tttttggact ccttgctgtt 3540 ggccaccttg tccttgctgt ctggctcatt
ctgtttcaac gccttttaat taacggagta 3600 ggtctcggtg tcggaagcga
cgccagatcc gtcatcctcc tttcgctctc caaagtagat 3660 acctccgacg
agctctcgga caatgatgaa gtcggtgccc tcaacgtttc ggatggggga 3720
gagatcggcg agcttgggcg acagcagctg gcagggtcgc aggttggcgt acaggttcag
3780 gtcctttcgc agcttgagga gaccctgctc gggtcgcacg tcggttcgtc
cgtcgggagt 3840 ggtccatacg gtgttggcag cgcctccgac agcaccgagc
ataatagagt cagcctttcg 3900 gcagatgtcg agagtagcgt cggtgatggg
ctcgccctcc ttctcaatgg cagctcctcc 3960 aatgagtcgg tcctcaaaca
caaactcggt gccggaggcc tcagcaacag acttgagcac 4020 cttgacggcc
tcggcaatca cctcggggcc acagaagtcg ccgccgagaa gaacaatctt 4080
cttggagtca gtcttggtct tcttagtttc gggttccatt gtggatgtgt gtggttgtat
4140 gtgtgatgtg gtgtgtggag tgaaaatctg tggctggcaa acgctcttgt
atatatacgc 4200 acttttgccc gtgctatgtg gaagactaaa cctccgaaga
ttgtgactca ggtagtgcgg 4260 tatcggctag ggacccaaac cttgtcgatg
ccgatagcat gcgacgtcgg gcccaattcg 4320 ccctatagtg agtcgtatta
caattcactg gccgtcgttt tacaacgtcg tgactgggaa 4380 aaccctggcg
ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 4440
aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
4500 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
cgcagcgtga 4560 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
tttcttccct tcctttctcg 4620 ccacgttcgc cggctttccc cgtcaagctc
taaatcgggg gctcccttta gggttccgat 4680 ttagtgcttt acggcacctc
gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 4740 ggccatcgcc
ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 4800
gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
4860 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
taacaaaaat 4920 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
ctgatgcggt attttctcct 4980 tacgcatctg tgcggtattt cacaccgcat
caggtggcac ttttcgggga aatgtgcgcg 5040 gaacccctat ttgtttattt
ttctaaatac attcaaatat gtatccgctc atgagacaat 5100 aaccctgata
aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 5160
gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5220 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
tacatcgaac 5280 tggatctcaa cagcggtaag atccttgaga gttttcgccc
cgaagaacgt tttccaatga 5340 tgagcacttt taaagttctg ctatgtggcg
cggtattatc ccgtattgac gccgggcaag 5400 agcaactcgg tcgccgcata
cactattctc agaatgactt ggttgagtac tcaccagtca 5460 cagaaaagca
tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 5520
tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5580 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
gaaccggagc 5640 tgaatgaagc cataccaaac gacgagcgtg acaccacgat
gcctgtagca atggcaacaa 5700 cgttgcgcaa actattaact ggcgaactac
ttactctagc ttcccggcaa caattaatag 5760 actggatgga ggcggataaa
gttgcaggac cacttctgcg ctcggccctt ccggctggct 5820 ggtttattgc
tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 5880
tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
5940 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
aagcattggt 6000 aactgtcaga ccaagtttac tcatatatac tttagattga
tttaaaactt catttttaat 6060 ttaaaaggat ctaggtgaag atcctttttg
ataatctcat gaccaaaatc ccttaacgtg 6120 agttttcgtt ccactgagcg
tcagaccccg tagaaaagat caaaggatct tcttgagatc 6180 ctttttttct
gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 6240
tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6300 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
ttcaagaact 6360 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
accagtggct gctgccagtg 6420 gcgataagtc gtgtcttacc gggttggact
caagacgata gttaccggat aaggcgcagc 6480 ggtcgggctg aacggggggt
tcgtgcacac agcccagctt ggagcgaacg acctacaccg 6540 aactgagata
cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 6600
cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6660 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
cttgagcgtc 6720 gatttttgtg atgctcgtca ggggggcgga gcctatggaa
aaacgccagc aacgcggcct 6780 ttttacggtt cctggccttt tgctggcctt
ttgctcacat gttctttcct gcgttatccc 6840 ctgattctgt ggataaccgt
attaccgcct ttgagtgagc tgataccgct cgccgcagcc 6900 gaacgaccga
gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 6960
cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcgcgcccac tgagctcgtc
7020 taacggactt gatatacaac caattaaaac aaatgaaaag aaatacagtt
ctttgtatca 7080 tttgtaacaa ttaccctgta caaactaagg tattgaaatc
ccacaatatt cccaaagtcc 7140 acccctttcc aaattgtcat gcctacaact
catataccaa gcactaacct accaaacacc 7200 actaaaaccc cacaaaatat
atcttaccga atatacagta acaagctacc accacactcg 7260 ttgggtgcag
tcgccagctt aaagatatct atccacatca gccacaactc ccttccttta 7320
ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta gacaagacac
7380 tttcaagaag actgtttcca aaacgtacca ctgtcctcca ctacaaacac
acccaatctg 7440 cttcttctag tcaaggttgc tacaccggta aattataaat
catcatttca ttagcagggc 7500 agggcccttt ttatagagtc ttatacacta
gcggaccctg ccggtagacc aacccgcagg 7560 cgcgtcagtt tgctccttcc
atcaatgcgt cgtagaaacg acttactcct tcttgagcag 7620 ctccttgacc
ttgttggcaa caagtctccg acctcggagg tggaggaaga gcctccgata 7680
tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac agcgtcaccg
7740 gcgggcttca tgttaagaga gaacttgagc atcatggcgg cagacagaat
ggtggcgtac 7800 gcaactaaca tgaatgaata cgatatacat caaagactat
gatacgcagt attgcacact 7860 gtacgagtaa gagcactagc cactgcactc
aagtgaaacc gttgcccggg tacgagtatg 7920 agtatgtaca gtatgtttag
tattgtactt ggacagtgct tgtatcgtac attctcaagt 7980 gtcaaacata
aatatccgtt gctatatcct cgcaccacca cgtagctcgc tatatccctg 8040
tgttgaatcc atccatcttg gattgccaat tgtgcacaca gaaccgggca ctcacttccc
8100 catccacact tgcggccgcg cctacttaag caacgggctt gataacagcg
gggggggtgc 8160 ccacgttgtt gcggttgcgg aagaacagaa cacccttacc
agcaccctcg gcaccagcgc 8220 tgggctcaac ccactggcac atacgcgcac
tgcggtacat ggcgcggatg aagccacgag 8280 gaccatcctg gacatcagcc
cggtagtgct tgcccatgat gggcttaatg gcctcggtgg 8340 cctcgtccgc
gttgtagaag gggatgctgc tgacgtagtg gtggaggaca tgagtctcga 8400
tgatgccgtg gagaaggtgg cggccgatga agcccatctc acggtcaatg gtagcagcgg
8460 caccacggac gaagttccac tcgtcgttgg tgtagtgggg aagggtaggg
tcggtgtgct 8520 ggaggaaggt gatggcaacg agccagtggt taacccagag
gtagggaaca aagtaccaga 8580 tggccatgtt gtagaaaccg aacttctgaa
cgaggaagta cagagcagtg gccatcagac 8640 cgataccaat atcgctgagg
acgatgagct tagcgtcact gttctcgtac agagggctgc 8700 ggggatcgaa
gtggttaaca ccaccgccga ggccgttatg cttgcccttg ccgcgaccct 8760
cacgctggcg ctcgtggtag ttgtggccgg taacattggt gatgaggtag ttgggccagc
8820 cnacgannnn ctcagtaaga tgagcgagct cgtgggtcat ctttccgaga
cgagtagcct 8880 gctgctcgcg ggttcgggga acgaagacca tgtcacgctc
catgttgcca gtggccttgt 8940 ggtgctttcg gtgggagatt tgccagctga
agtaggggac aaggagggaa gagtgaagaa 9000 cccagccagt aatgtcgttg
atgatgcgag aatcggagaa agcaccgtga ccgcactcat 9060 gggcaataac
ccagagacca gtaccgaaaa gaccctgaag aacggtgtac acggcccaca 9120
gaccagcgcg ggcgggggtg gaggggatat attcgggggt cacaaagttg taccagatgc
9180 tgaaagtggt agtcaggagg acaatgtcgc ggaggatata accgtatccc
ttgagagcgg 9240 agcgcttgaa gcagtgctta gggatggcat tgtagatgtc
cttgatggta aagtcgggaa 9300 cctcgaactg gttgccgtag gtgtcgagca
tgacaccata ctcggacttg ggcttggcga 9360 tatcaacctc ggacatggac
gagagcgatg tggaagaggc cgagtggcgg ggagagtctg 9420 aaggagagac
ggcggcagac tcagaatccg tcacagtagt tgaggtgacg gtgcgtctaa 9480
gcgcagggtt ctgcttgggc agagccgaag tggacgccat ggttgatgtg tgtttaattc
9540 aagaatgaat atagagaaga gaagaagaaa aaagattcaa ttgagccggc
gatgcagacc 9600 cttatataaa tgttgccttg gacagacgga gcaagcccgc
ccaaacctac gttcggtata 9660 atatgttaag ctttttaaca caaaggtttg
gcttggggta acctgatgtg gtgcaaaaga 9720 ccgggcgttg gcgagccatt
gcgcgggcga atggggccgt gactcgtctc aaattcgagg 9780 gcgtgcctca
attcgtgccc ccgtggcttt ttcccgccgt ttccgccccg tttgcaccac 9840
tgcagccgct tctttggttc ggacaccttg ctgcgagcta ggtgccttgt gctacttaaa
9900 aagtggcctc ccaacaccaa catgacatga gtgcgtgggc caagacacgt
tggcggggtc 9960 gcagtcggct caatggcccg gaaaaaacgc tgctggagct
ggttcggacg cagtccgccg 10020 cggcgtatgg atatccgcaa ggttccatag
cgccattgcc ctccgtcggc gtctatcccg 10080 caacctctaa atagagcggg
aatataaccc aagcttcttt tttttccttt aacacgcaca 10140 cccccaacta
tcatgttgct gctgctgttt gactctactc tgtggagggg tgctcccacc 10200
caacccaacc tacaggtgga tccggcgctg tgattggctg ataagtctcc tatccggact
10260 aattctgacc aatgggacat gcgcgcagga cccaaatgcc gcaattacgt
aaccccaacg 10320 aaatgcctac ccctctttgg agcccagcgg ccccaaatcc
ccccaagcag cccggttcta 10380 ccggcttcca tctccaagca caagcagccc
ggttctaccg gcttccatct ccaagcaccc 10440 ctttctccac accccacaaa
aagacccgtg caggacatcc tactgcgtcg acatcattta 10500 aattccttca
cttcaagttc attcttcatc tgcttctgtt ttactttgac aggcaaatga 10560
agacatggta cgacttgatg gaggccaaga acgccatttc accccgagac accgaagtgc
10620 ctgaaatcct ggctgccccc attgataaca tcggaaacta cggtattccg
gaaagtgtat 10680 atagaacctt tccccagctt gtgtctgtgg atatggatgg
tgtaatcccc tttgagtact 10740 cgtcttggct tctctccgag cagtatgagg
ctctctaatc tagcgcattt aatatctcaa 10800 tgtatttata tatttatctt
ctcatgcggc cgctcactga atctttttgg ctcccttgtg 10860 cttcctgacg
atatacgttt gcacatagaa attcaagaac aaacacaaga ctgtgccaac 10920
ataaaagtaa ttgaagaacc agccaaacat cctcatccca tcttggcgat aacagggaat
10980 gttcctgtac ttccagacaa tgtagaaacc aacattgaat tgaatgatct
gcattgatgt 11040 aatcagggat tttggcatgg ggaacttcag cttgatcaat
ctggtccaat aataaccgta 11100 catgatccag tggatgaaac cattcaacag
cacaaaaatc caaacagctt catttcggta 11160 attatagaac agccacatat
ccatcggtgc ccccaaatga tggaagaatt gcaaccaggt 11220 cagaggcttg
cccatcagtg gcaaatagaa ggagtcaata tactccagga acttgctcaa 11280
atagaacaac tgcgtggtga tcctgaagac gttgttgtca aaagccttct cgcagttgtc
11340 agacataaca ccgatggtgt acatggcata tgccattgag aggaatgatc
ccaacgaata 11400 aatggacatg agaaggttgt aattggtgaa aacaaacttc
atacgagact gaccttttgg 11460 accaaggggg ccaagagtga acttcaagat
gacaaatgcg atggacaagt aaagcacctc 11520 acagtgactg gcatcactcc
agagttgggc ataatcaact ggttgggtaa aacttcctgc 11580 ccaattgaga
ctatttcatt caccacctcc atggccattg ctgtagatat gtcttgtgtg 11640
taagggggtt ggggtggttg tttgtgttct tgacttttgt gttagcaagg gaagacgggc
11700 aaaaaagtga gtgtggttgg gagggagaga cgagccttat atataatgct
tgtttgtgtt 11760 tgtgcaagtg gacgccgaaa cgggcaggag ccaaactaaa
caaggcagac aatgcgagct 11820 taattggatt gcctgatggg caggggttag
ggctcgatca atgggggtgc gaagtgacaa 11880 aattgggaat taggttcgca
agcaaggctg acaagacttt ggcccaaaca tttgtacgcg 11940 gtggacaaca
ggagccaccc atcgtctgtc acgggctagc cggtcgtgcg tcctgtcagg 12000
ctccacctag gctccatgcc actccataca atcccactag tgtaccgcta ggccgctttt
12060 agctcccatc taagaccccc ccaaaacctc cactgtacag tgcactgtac
tgtgtggcga 12120 tcaagggcaa gggaaaaaag gcgcaaacat gcacgcatgg
aatgacgtag gtaaggcgtt 12180 actagactga aaagtggcac atttcggcgt
gccaaagggt cctaggtgcg tttcgcgagc 12240 tgggcgccag gccaagccgc
tccaaaacgc ctctccgact ccctccagcg gcctccatat 12300 ccccatccct
ctccacagca atgttgttaa gccttgcaaa cgaaaaaata gaaaggctaa 12360
taagcttcca atattgtggt gtacgctgca taacgcaaca atgagcgcca aacaacacac
12420 acacacagca cacagcagca ttaaccacga tgaacagcat gacattacag
gtgggtgtgt 12480 aatcagggcc ctgattgctg gtggtgggag cccccatcat
gggcagatct gcgtacactg 12540 tttaaacagt gtacgcagat ctactataga
ggaacattta aattgccccg gagaagacgg 12600 ccaggccgcc tagatgacaa
attcaacaac tcacagctga ctttctgcca ttgccactag 12660 gggggggcct
ttttatatgg ccaagccaag ctctccacgt cggttgggct gcacccaaca 12720
ataaatgggt agggttgcac caacaaaggg atgggatggg gggtagaaga tacgaggata
12780 acggggctca atggcacaaa taagaacgaa tactgccatt aagactcgtg
atccagcgac 12840 tgacaccatt gcatcatcta agggcctcaa aactacctcg
gaactgctgc gctgatctgg 12900 acaccacaga ggttccgagc actttaggtt
gcaccaaatg tcccaccagg tgcaggcaga 12960 aaacgctgga acagcgtgta
cagtttgtct taacaaaaag tgagggcgct gaggtcgagc 13020 agggtggtgt
gacttgttat agcctttaga gctgcgaaag cgcgtatgga tttggctcat 13080
caggccagat tgagggtctg tggacacatg tcatgttagt gtacttcaat cgccccctgg
13140 atatagcccc gacaataggc cgtggcctca tttttttgcc ttccgcacat
ttccattgct 13200 cgatacccac accttgcttc tcctgcactt gccaacctta
atactggttt acattgacca 13260 acatcttaca agcggggggc ttgtctaggg
tatatataaa cagtggctct cccaatcggt 13320 tgccagtctc ttttttcctt
tctttcccca cagattcgaa atctaaacta cacatcacag 13380 aattccgagc
cgtgagtatc cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat 13440
gacacaatcc gaaagtcgct agcaacacac actctctaca caaactaacc cagctctggt
13500 accatggagg tcgtgaacga aatcgtctcc attggccagg aggttcttcc
caaggtcgac 13560 tatgctcagc tctggtctga tgcctcgcac tgcgaggtgc
tgtacctctc catcgccttc 13620 gtcatcctga agttcaccct tggtcctctc
ggacccaagg gtcagtctcg aatgaagttt 13680 gtgttcacca actacaacct
gctcatgtcc atctactcgc tgggctcctt cctctctatg 13740 gcctacgcca
tgtacaccat tggtgtcatg tccgacaact gcgagaaggc tttcgacaac 13800
aatgtcttcc gaatcaccac tcagctgttc tacctcagca agttcctcga gtacattgac
13860 tccttctatc tgcccctcat gggcaagcct ctgacctggt tgcagttctt
tcaccatctc 13920 ggagctccta tggacatgtg gctgttctac aactaccgaa
acgaagccgt ttggatcttt 13980 gtgctgctca acggcttcat tcactggatc
atgtacggct actattggac ccgactgatc 14040 aagctcaagt tccctatgcc
caagtccctg attacttcta tgcagatcat tcagttcaac 14100 gttggcttct
acatcgtctg gaagtaccgg aacattccct gctaccgaca agatggaatg 14160
agaatgtttg gctggttttt caactacttc tacgttggta ctgtcctgtg tctgttcctc
14220 aacttctacg tgcagaccta catcgtccga aagcacaagg gagccaaaaa
gattcagtga 14280 gcggccgcat gtacatacaa gattatttat agaaatgaat
cgcgatcgaa caaagagtac 14340 gagtgtacga gtaggggatg atgataaaag
tggaagaagt tccgcatctt tggatttatc 14400 aacgtgtagg acgatacttc
ctgtaaaaat gcaatgtctt taccataggt tctgctgtag 14460 atgttattaa
ctaccattaa catgtctact tgtacagttg cagaccagtt ggagtataga 14520
atggtacact taccaaaaag tgttgatggt tgtaactacg atatataaaa ctgttgacgg
14580 gatccccgct gatatgccta aggaacaatc aaagaggaag atattaattc
agaatgctag 14640 tatacagtta gggat 14655 <210> SEQ ID NO 64
<211> LENGTH: 777 <212> TYPE: DNA <213> ORGANISM:
Euglena gracilis <220> FEATURE: <221> NAME/KEY:
misc_feature <223> OTHER INFORMATION: synthetic delta-9
elongase (codon-optimized) <300> PUBLICATION INFORMATION:
<302> TITLE: DELTA-9 ELONGASES AND THEIR USE IN MAKING
POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT NUMBER: WO
2007/061742 <311> PATENT FILING DATE: 2006-11-16 <312>
PUBLICATION DATE: 2007-05-31 <313> RELEVANT RESIDUES IN SEQ
ID NO: (1)..(777) <400> SEQUENCE: 64 atggaggtcg tgaacgaaat
cgtctccatt ggccaggagg ttcttcccaa ggtcgactat 60 gctcagctct
ggtctgatgc ctcgcactgc gaggtgctgt acctctccat cgccttcgtc 120
atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg
180 ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct
ctctatggcc 240 tacgccatgt acaccattgg tgtcatgtcc gacaactgcg
agaaggcttt cgacaacaat 300 gtcttccgaa tcaccactca gctgttctac
ctcagcaagt tcctcgagta cattgactcc 360 ttctatctgc ccctcatggg
caagcctctg acctggttgc agttctttca ccatctcgga 420 gctcctatgg
acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg 480
ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag
540 ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca
gttcaacgtt 600 ggcttctaca tcgtctggaa gtaccggaac attccctgct
accgacaaga tggaatgaga 660 atgtttggct ggtttttcaa ctacttctac
gttggtactg tcctgtgtct gttcctcaac 720 ttctacgtgc agacctacat
cgtccgaaag cacaagggag ccaaaaagat tcagtga 777 <210> SEQ ID NO
65 <211> LENGTH: 258 <212> TYPE: PRT <213>
ORGANISM: Euglena gracilis <220> FEATURE: <221>
NAME/KEY: MISC_FEATURE <222> LOCATION: (1)..(258) <223>
OTHER INFORMATION: delta-9 elongase (EgD9e) <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-9 ELONGASES AND THEIR USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: WO 2007/061742 <311> PATENT FILING DATE: 2006-11-16
<312> PUBLICATION DATE: 2007-05-31 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(258) <400> SEQUENCE: 65 Met Glu
Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 1 5 10 15
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 20
25 30 Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly
Pro 35 40 45 Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe
Thr Asn Tyr 50 55 60 Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser
Phe Leu Ser Met Ala 65 70 75 80 Tyr Ala Met Tyr Thr Ile Gly Val Met
Ser Asp Asn Cys Glu Lys Ala 85 90 95 Phe Asp Asn Asn Val Phe Arg
Ile Thr Thr Gln Leu Phe Tyr Leu Ser 100 105 110 Lys Phe Leu Glu Tyr
Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 115 120 125 Pro Leu Thr
Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 130 135 140 Met
Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 145 150
155 160 Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp
Thr 165 170 175 Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu
Ile Thr Ser 180 185 190 Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr
Ile Val Trp Lys Tyr 195 200 205 Arg Asn Ile Pro Cys Tyr Arg Gln Asp
Gly Met Arg Met Phe Gly Trp 210 215 220 Phe Phe Asn Tyr Phe Tyr Val
Gly Thr Val Leu Cys Leu Phe Leu Asn 225 230 235 240 Phe Tyr Val Gln
Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 245 250 255 Ile Gln
<210> SEQ ID NO 66 <211> LENGTH: 34 <212> TYPE:
DNA <213> ORGANISM: Escherichia coli <400> SEQUENCE: 66
ataacttcgt ataatgtatg ctatacgaag ttat 34 <210> SEQ ID NO 67
<211> LENGTH: 828 <212> TYPE: DNA <213> ORGANISM:
Mortierella alpina <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(828) <223> OTHER INFORMATION:
synthetic C16/18 elongase (codon-optimized) <300> PUBLICATION
INFORMATION: <302> TITLE: A MORTIERELLA ALPINA C16/18 FATTY
ACID ELONGASE <310> PATENT DOCUMENT NUMBER: US
2007/0087420-A1 <311> PATENT FILING DATE: 2005-10-19
<312> PUBLICATION DATE: 2007-04-19 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(828) <400> SEQUENCE: 67 atg gag
tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat 48 Met Glu
Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 1 5 10 15
gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac 96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 20
25 30 act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc
cga 144 Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser
Arg 35 40 45 gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga
acc cag tcc 192 Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg
Thr Gln Ser 50 55 60 gga gct gcc atg act gcc ttc gtc ttt gtg cac
aac ctc att ctg tgt 240 Gly Ala Ala Met Thr Ala Phe Val Phe Val His
Asn Leu Ile Leu Cys 65 70 75 80 gtc tac tct ggc atc acc ttc tac tac
atg ttt cct gct atg gtc aag 288 Val Tyr Ser Gly Ile Thr Phe Tyr Tyr
Met Phe Pro Ala Met Val Lys 85 90 95 aac ttc cga acc cac aca ctg
cac gaa gcc tac tgc gac acg gat cag 336 Asn Phe Arg Thr His Thr Leu
His Glu Ala Tyr Cys Asp Thr Asp Gln 100 105 110 tcc ctc tgg aac aac
gca ctt ggc tac tgg ggt tac ctc ttc tac ctg 384 Ser Leu Trp Asn Asn
Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 115 120 125 tcc aag ttc
tac gag gtc att gac acc atc atc atc atc ctg aag gga 432 Ser Lys Phe
Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 130 135 140 cga
cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att 480 Arg
Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 145 150
155 160 acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc
ttt 528 Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile
Phe 165 170 175 gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt
tac tat gcc 576 Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys
Tyr Tyr Ala 180 185 190 ttc acc tct atc gga ttc cat cct cct ggc aaa
aag tac ctg act tcg 624 Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys
Lys Tyr Leu Thr Ser 195 200 205 atg cag att act cag ttt ctg gtc ggt
atc acc att gcc gtg tcc tac 672 Met Gln Ile Thr Gln Phe Leu Val Gly
Ile Thr Ile Ala Val Ser Tyr 210 215 220 ctc ttc gtt cct ggc tgc atc
cga aca ccc ggt gct cag atg gct gtc 720 Leu Phe Val Pro Gly Cys Ile
Arg Thr Pro Gly Ala Gln Met Ala Val 225 230 235 240 tgg atc aac gtc
ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac 768 Trp Ile Asn Val
Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 245 250 255 ttt gcc
aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa 816 Phe Ala
Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 260 265 270
aag gct cag taa 828 Lys Ala Gln 275 <210> SEQ ID NO 68
<211> LENGTH: 275 <212> TYPE: PRT <213> ORGANISM:
Mortierella alpina <400> SEQUENCE: 68 Met Glu Ser Gly Pro Met
Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 1 5 10 15 Asp Phe Phe Met
Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 20 25 30 Thr Ala
Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 35 40 45
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 50
55 60 Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu
Cys 65 70 75 80 Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala
Met Val Lys 85 90 95 Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr
Cys Asp Thr Asp Gln 100 105 110 Ser Leu Trp Asn Asn Ala Leu Gly Tyr
Trp Gly Tyr Leu Phe Tyr Leu 115 120 125 Ser Lys Phe Tyr Glu Val Ile
Asp Thr Ile Ile Ile Ile Leu Lys Gly 130 135 140 Arg Arg Ser Ser Leu
Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 145 150 155 160 Thr Met
Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 165 170 175
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 180
185 190 Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr
Ser 195 200 205 Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala
Val Ser Tyr 210 215 220 Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly
Ala Gln Met Ala Val 225 230 235 240 Trp Ile Asn Val Gly Tyr Leu Phe
Pro Leu Thr Tyr Leu Phe Val Asp 245 250 255 Phe Ala Lys Arg Thr Tyr
Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 260 265 270 Lys Ala Gln 275
<210> SEQ ID NO 69 <211> LENGTH: 8739 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY116 <400> SEQUENCE:
69 ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc
gggtggacgt 60 ctagaggtac ctagcaatta acagatagtt tgccggtgat
aattctctta acctcccaca 120 ctcctttgac ataacgattt atgtaacgaa
actgaaattt gaccagatat tgtgtccgcg 180 gtggagctcc agcttttgtt
ccctttagtg agggtttaaa cgagcttggc gtaatcatgg 240 tcatagctgt
ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc 300
ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
360 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
ttaatgaatc 420 ggccaacgcg cggggagagg cggtttgcgt attgggcgct
cttccgcttc ctcgctcact 480 gactcgctgc gctcggtcgt tcggctgcgg
cgagcggtat cagctcactc aaaggcggta 540 atacggttat ccacagaatc
aggggataac gcaggaaaga acatgtgagc aaaaggccag 600 caaaaggcca
ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 660
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
720 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
tccgaccctg 780 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
gcgtggcgct ttctcatagc 840 tcacgctgta ggtatctcag ttcggtgtag
gtcgttcgct ccaagctggg ctgtgtgcac 900 gaaccccccg ttcagcccga
ccgctgcgcc ttatccggta actatcgtct tgagtccaac 960 ccggtaagac
acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1020
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
1080 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
aagagttggt 1140 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
gtttttttgt ttgcaagcag 1200 cagattacgc gcagaaaaaa aggatctcaa
gaagatcctt tgatcttttc tacggggtct 1260 gacgctcagt ggaacgaaaa
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 1320 atcttcacct
agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1380
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
1440 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
tacgatacgg 1500 gagggcttac catctggccc cagtgctgca atgataccgc
gagacccacg ctcaccggct 1560 ccagatttat cagcaataaa ccagccagcc
ggaagggccg agcgcagaag tggtcctgca 1620 actttatccg cctccatcca
gtctattaat tgttgccggg aagctagagt aagtagttcg 1680 ccagttaata
gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1740
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
1800 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
cagaagtaag 1860 ttggccgcag tgttatcact catggttatg gcagcactgc
ataattctct tactgtcatg 1920 ccatccgtaa gatgcttttc tgtgactggt
gagtactcaa ccaagtcatt ctgagaatag 1980 tgtatgcggc gaccgagttg
ctcttgcccg gcgtcaatac gggataatac cgcgccacat 2040 agcagaactt
taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 2100
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
2160 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
aaatgccgca 2220 aaaaagggaa taagggcgac acggaaatgt tgaatactca
tactcttcct ttttcaatat 2280 tattgaagca tttatcaggg ttattgtctc
atgagcggat acatatttga atgtatttag 2340 aaaaataaac aaataggggt
tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 2400 tgtagcggcg
cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2460
gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
2520 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
tagtgcttta 2580 cggcacctcg accccaaaaa acttgattag ggtgatggtt
cacgtagtgg gccatcgccc 2640 tgatagacgg tttttcgccc tttgacgttg
gagtccacgt tctttaatag tggactcttg 2700 ttccaaactg gaacaacact
caaccctatc tcggtctatt cttttgattt ataagggatt 2760 ttgccgattt
cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 2820
tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg
2880 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
ggatgtgctg 2940 caaggcgatt aagttgggta acgccagggt tttcccagtc
acgacgttgt aaaacgacgg 3000 ccagtgaatt gtaatacgac tcactatagg
gcgaattggg taccgggccc cccctcgagg 3060 tcgatggtgt cgataagctt
gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa 3120 ggaaacctaa
ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg 3180
ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat
3240 gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc
cgcctccaac 3300 tgatgttctc aatatttaag gggtcatctc gcattgttta
ataataaaca gactccatct 3360 accgcctcca aatgatgttc tcaaaatata
ttgtatgaac ttatttttat tacttagtat 3420 tattagacaa cttacttgct
ttatgaaaaa cacttcctat ttaggaaaca atttataatg 3480 gcagttcgtt
catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct 3540
taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa
3600 aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa
gaacagctat 3660 tcacacgtta ctattgagat tattattgga cgagaatcac
acactcaact gtctttctct 3720 cttctagaaa tacaggtaca agtatgtact
attctcattg ttcatacttc tagtcatttc 3780 atcccacata ttccttggat
ttctctccaa tgaatgacat tctatcttgc aaattcaaca 3840 attataataa
gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg 3900
cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat
3960 ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt
taattttttg 4020 cttaaattca atcccccctc gttcagtgtc aactgtaatg
gtaggaaatt accatacttt 4080 tgaagaagca aaaaaaatga aagaaaaaaa
aaatcgtatt tccaggttag acgttccgca 4140 gaatctagaa tgcggtatgc
ggtacattgt tcttcgaacg taaaagttgc gctccctgag 4200 atattgtaca
tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg 4260
atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta
4320 ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc
aattaatcat 4380 agacttatga atctgcacgg tgtgcgctgc gagttacttt
tagcttatgc atgctacttg 4440 ggtgtaatat tgggatctgt tcggaaatca
acggatgctc aaccgatttc gacagtaatt 4500 aattaatttg aatcgaatcg
gagcctaaaa tgaacccgag tatatctcat aaaattctcg 4560 gtgagaggtc
tgtgactgtc agtacaaggt gccttcatta tgccctcaac cttaccatac 4620
ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg ccaaaagcca aggcactgag
4680 ctcgtctaac ggacttgata tacaaccaat taaaacaaat gaaaagaaat
acagttcttt 4740 gtatcatttg taacaattac cctgtacaaa ctaaggtatt
gaaatcccac aatattccca 4800 aagtccaccc ctttccaaat tgtcatgcct
acaactcata taccaagcac taacctacca 4860 aacaccacta aaaccccaca
aaatatatct taccgaatat acagtaacaa gctaccacca 4920 cactcgttgg
gtgcagtcgc cagcttaaag atatctatcc acatcagcca caactccctt 4980
cctttaataa accgactaca cccttggcta ttgaggttat gagtgaatat actgtagaca
5040 agacactttc aagaagactg tttccaaaac gtaccactgt cctccactac
aaacacaccc 5100 aatctgcttc ttctagtcaa ggttgctaca ccggtaaatt
ataaatcatc atttcattag 5160 cagggcaggg ccctttttat agagtcttat
acactagcgg accctgccgg tagaccaacc 5220 cgcaggcgcg tcagtttgct
ccttccatca atgcgtcgta gaaacgactt actccttctt 5280 gagcagctcc
ttgaccttgt tggcaacaag tctccgacct cggaggtgga ggaagagcct 5340
ccgatatcgg cggtagtgat accagcctcg acggactcct tgacggcagc ctcaacagcg
5400 tcaccggcgg gcttcatgtt aagagagaac ttgagcatca tggcggcaga
cagaatggtg 5460 gcaatggggt tgaccttctg cttgccgaga tcgggggcag
atccgtgaca gggctcgtac 5520 agaccgaacg cctcgttggt gtcgggcaga
gaagccagag aggcggaggg cagcagaccc 5580 agagaaccgg ggatgacgga
ggcctcgtcg gagatgatat cgccaaacat gttggtggtg 5640 atgatgatac
cattcatctt ggagggctgc ttgatgagga tcatggcggc cgagtcgatc 5700
agctggtggt tgagctcgag ctgggggaat tcgtccttga ggactcgagt gacagtcttt
5760 cgccaaagtc gagaggaggc cagcacgttg gccttgtcaa gagaccacac
gggaagaggg 5820 gggttgtgct gaagggccag gaaggcggcc attcgggcaa
ttcgctcaac ctcaggaacg 5880 gagtaggtct cggtgtcgga agcgacgcca
gatccgtcat cctcctttcg ctctccaaag 5940 tagatacctc cgacgagctc
tcggacaatg atgaagtcgg tgccctcaac gtttcggatg 6000 ggggagagat
cggcgagctt gggcgacagc agctggcagg gtcgcaggtt ggcgtacagg 6060
ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc gcacgtcggt tcgtccgtcg
6120 ggagtggtcc atacggtgtt ggcagcgcct ccgacagcac cgagcataat
agagtcagcc 6180 tttcggcaga tgtcgagagt agcgtcggtg atgggctcgc
cctccttctc aatggcagct 6240 cctccaatga gtcggtcctc aaacacaaac
tcggtgccgg aggcctcagc aacagacttg 6300 agcaccttga cggcctcggc
aatcacctcg gggccacaga agtcgccgcc gagaagaaca 6360 atcttcttgg
agtcagtctt ggtcttctta gtttcgggtt ccattgtgga tgtgtgtggt 6420
tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct ggcaaacgct cttgtatata
6480 tacgcacttt tgcccgtgct atgtggaaga ctaaacctcc gaagattgtg
actcaggtag 6540 tgcggtatcg gctagggacc caaaccttgt cgatgccgat
agcgctatcg aacgtacccc 6600 agccggccgg gagtatgtcg gaggggacat
acgagatcgt caagggtttg tggccaactg 6660 gtatttaaat gtagctaacg
gtagcaggcg aactactggt acatacctcc cccggaatat 6720 gtacaggcat
aatgcgtatc tgtgggacat gtggtcgttg cgccattatg taagcagcgt 6780
gtactcctct gactgtccat atggtttgct ccatctcacc ctcatcgttt tcattgttca
6840 caggcggcca caaaaaaact gtcttctctc cttctctctt cgccttagtc
tactcggacc 6900 agttttagtt tagcttggcg ccactggata aatgagacct
caggccttgt gatgaggagg 6960 tcacttatga agcatgttag gaggtgcttg
tatggataga gaagcaccca aaataataag 7020 aataataata aaacaggggg
cgttgtcatt tcatatcgtg ttttcaccat caatacacct 7080 ccaaacaatg
cccttcatgt ggccagcccc aatattgtcc tgtagttcaa ctctatgcag 7140
ctcgtatctt attgagcaag taaaactctg tcagccgata ttgcccgacc cgcgacaagg
7200 gtcaacaagg tggtgtaagg ccttcgcaga agtcaaaact gtgccaaaca
aacatctaga 7260 gtctctttgg tgtttctcgc atatatttwa tcggctgtct
tacgtatttg cgcctcggta 7320 ccggactaat ttcggatcat ccccaatacg
ctttttcttc gcagctgtca acagtgtcca 7380 tgatctatcc acctaaatgg
gtcatatgag gcgtataatt tcgtggtgct gataataatt 7440 cccatatatt
tgacacaaaa cttccccccc tagacataca tctcacaatc tcacttcttg 7500
tgcttctgtc acacatctcc tccagctgac ttcaactcac acctctgccc cagttggtct
7560 acagcggtat aaggtttctc cgcatagagg tgcaccactc ctcccgatac
ttgtttgtgt 7620 gacttgtggg tcacgacata tatatctaca cacattgcgc
caccctttgg ttcttccagc 7680 acaacaaaaa cacgacacgc taaccatggc
caatttactg accgtacacc aaaatttgcc 7740 tgcattaccg gtcgatgcaa
cgagtgatga ggttcgcaag aacctgatgg acatgttcag 7800 ggatcgccag
gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg 7860
ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg
7920 cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
agcaacattt 7980 gggccagcta aacatgcttc atcgtcggtc cgggctgcca
cgaccaagtg acagcaatgc 8040 tgtttcactg gttatgcggc ggatccgaaa
agaaaacgtt gatgccggtg aacgtgcaaa 8100 acaggctcta gcgttcgaac
gcactgattt cgaccaggtt cgttcactca tggaaaatag 8160 cgatcgctgc
caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt 8220
acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag
8280 aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
tagagaaggc 8340 acttagcctg ggggtaacta aactggtcga gcgatggatt
tccgtctctg gtgtagctga 8400 tgatccgaat aactacctgt tttgccgggt
cagaaaaaat ggtgttgccg cgccatctgc 8460 caccagccag ctatcaactc
gcgccctgga agggattttt gaagcaactc atcgattgat 8520 ttacggcgct
aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg 8580
tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc
8640 tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
atagtgaaac 8700 aggggcaatg gtgcgcctgc tggaagatgg cgattaagc 8739
<210> SEQ ID NO 70 <211> LENGTH: 15304 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pKO2UF8289
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (5601)..(5601) <223> OTHER INFORMATION: n is a, c,
g, or t <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (5606)..(5609) <223> OTHER INFORMATION:
n is a, c, g, or t <400> SEQUENCE: 70 cgatcgagga agaggacaag
cggctgcttc ttaagtttgt gacatcagta tccaaggcac 60 cattgcaagg
attcaaggct ttgaacccgt catttgccat tcgtaacgct ggtagacagg 120
ttgatcggtt ccctacggcc tccacctgtg tcaatcttct caagctgcct gactatcagg
180 acattgatca acttcggaag aaacttttgt atgccattcg atcacatgct
ggtttcgatt 240 tgtcttagag gaacgcatat acagtaatca tagagaataa
acgatattca tttattaaag 300 tagatagttg aggtagaagt tgtaaagagt
gataaatagc ggccgctcac tgaatctttt 360 tggctccctt gtgcttcctg
acgatatacg tttgcacata gaaattcaag aacaaacaca 420 agactgtgcc
aacataaaag taattgaaga accagccaaa catcctcatc ccatcttggc 480
gataacaggg aatgttcctg tacttccaga caatgtagaa accaacattg aattgaatga
540 tctgcattga tgtaatcagg gattttggca tggggaactt cagcttgatc
aatctggtcc 600 aataataacc gtacatgatc cagtggatga aaccattcaa
cagcacaaaa atccaaacag 660 cttcatttcg gtaattatag aacagccaca
tatccatcgg tgcccccaaa tgatggaaga 720 attgcaacca ggtcagaggc
ttgcccatca gtggcaaata gaaggagtca atatactcca 780 ggaacttgct
caaatagaac aactgcgtgg tgatcctgaa gacgttgttg tcaaaagcct 840
tctcgcagtt gtcagacata acaccgatgg tgtacatggc atatgccatt gagaggaatg
900 atcccaacga ataaatggac atgagaaggt tgtaattggt gaaaacaaac
ttcatacgag 960 actgaccttt tggaccaagg gggccaagag tgaacttcaa
gatgacaaat gcgatggaca 1020 agtaaagcac ctcacagtga ctggcatcac
tccagagttg ggcataatca actggttggg 1080 taaaacttcc tgcccaattg
agactatttc attcaccacc tccatggtta gcgtgtcgtg 1140 tttttgttgt
gctggaagaa ccaaagggtg gcgcaatgtg tgtagatata tatgtcgtga 1200
cccacaagtc acacaaacaa gtatcgggag gagtggtgca cctctatgcg gagaaacctt
1260 ataccgctgt agaccaactg gggcagaggt gtgagttgaa gtcagctgga
ggagatgtgt 1320 gacagaagca caagaagtga gattgtgaga tgtatgtcta
gggggggaag ttttgtgtca 1380 aatatatggg aattattatc agcaccacga
aattatacgc ctcatatgac ccatttaggt 1440 ggatagatca tggacactgt
tgacagctgc gaagaaaaag cgtattgggg atgatccgaa 1500 attagtccgg
taccgaggcg caaatacgta agacagccga twaaatatat gcgagaaaca 1560
ccaaagagac tctagatgtt tgtttggcac agttttgact tctgcgaagg ccttacacca
1620 ccttgttgac ccttgtcgcg ggtcgggcaa tatcggctga cagagtttta
cttgctcaat 1680 aagatacgag ctgcatagag ttgaactaca ggacaatatt
ggggctggcc acatgaaggg 1740 cattgtttgg aggtgtattg atggtgaaaa
cacgatatga aatgacaacg ccccctgttt 1800 tattattatt cttattattt
tgggtgcttc tctatccata caagcacctc ctaacatgct 1860 tcataagtga
cctcctcatc acaaggcctg aggtctcatt tatccagtgg cgccaagcta 1920
aactaaaact ggtccgagta gactaaggcg aagagagaag gagagaagac agtttttttg
1980 tggccgcctg tgaacaatga aaacgatgag ggtgagatgg agcaaaccat
atggtttaaa 2040 cagtcagagg agtacacgct gcttacataa tggcgcaacg
accacatgtc ccacagatac 2100 gcatcgattc gattcaaatt aattaaaagg
cgttgaaaca gaatgagcca gacagcaagg 2160 acaaggtggc caacagcaag
gagtccaaaa agccctctat tgacgagatc cacgatgtta 2220 ttgctcatga
ggtttccgag ctcgatgctg ggaagaagaa gtgatttgta tataagaaat 2280
aaatgagata tagtaaagga gtgcaagaga atggcaaggt ggtcaaattc tatattactt
2340 gcagtcactg gttcctcgtt gacatgaatg aagttaccgt tggcatagct
gatttaatat 2400 ataactgtcc aactaactct cacctagata taacccatgt
gtgtgtttcc aatcatcaat 2460 gcggccgctt actgagcctt ggcaccgggc
tgcttctcgg ccattcgagc gaactgggac 2520 aggtatcgga gcaggatgac
gagaccttca tggggcagag ggtttcggta ggggaggttg 2580 tgcttctggc
acagctgttc cacctggtag gaaacggcag tgaggttgtg tcgaggcagg 2640
gtgggccaga gatggtgctc gatctggtag ttcaggcctc caaagaacca gtcagtaatg
2700 atgcctcgtc gaatgttcat ggtctcatgg atctgaccca cagagaagcc
atgtccgtcc 2760 cagacggaat caccgatctt ctccagaggg tagtggttca
tgaagaccac gatggcaatt 2820 ccgaagccac cgacgagctc ggaaacaaag
aacaccagca tcgaggtcag gatggagggc 2880 ataaagaaga ggtggaacag
ggtcttgaga gtccagtgca gagcgagtcc aatggcctct 2940 ttcttgtact
gagatcggta gaactggttg tctcggtcct tgagggatcg aacggtcagc 3000
acagactgga aacaccagat gaatcgcagg agaatacaga tgaccaggaa atagtactgt
3060 tggaactgaa tgagctttcg ggagatggga gaagctcgag tgacatcgtc
ctcggaccag 3120 gcgagcagag gcaggttatc aatgtcggga tcgtgaccct
gaacgttggt agcagaatga 3180 tgggcgttgt gtctgtcctt ccaccaggtc
acggagaagc cctggagtcc gttgccaaag 3240 accagaccca ggacgttatt
ccagtttcgg ttcttgaagg tctggtggtg gcagatgtca 3300 tgagacagcc
atcccatttg ctggtagtgc ataccgagca cgagagcacc aatgaagtac 3360
aggtggtact ggaccagcat gaagaaggca agcacgccaa gacccagggt ggtcaagatc
3420 ttgtacgagt accagagggg agaggcgtca aacatgccag tggcgatcag
ctcttctcgg 3480 agctttcgga aatcctcctg agcttcgttg acggcagcct
ggggaggcag ctcggaagcc 3540 tggttgatct tgggcattcg cttgagcttg
tcgaaggctt cctgagagtg cataaccatg 3600 aaggcgtcag tagcatctcg
tccctggtag ttctcaatga tttcagctcc accagggtgg 3660 aagttcaccc
aagcggagac gtcgtacacc tttccgtcga tgacgagggg cagagcctgt 3720
cgagaagcct tcaccatggc cattgctgta gatatgtctt gtgtgtaagg gggttggggt
3780 ggttgtttgt gttcttgact tttgtgttag caagggaaga cgggcaaaaa
agtgagtgtg 3840 gttgggaggg agagacgagc cttatatata atgcttgttt
gtgtttgtgc aagtggacgc 3900 cgaaacgggc aggagccaaa ctaaacaagg
cagacaatgc gagcttaatt ggattgcctg 3960 atgggcaggg gttagggctc
gatcaatggg ggtgcgaagt gacaaaattg ggaattaggt 4020 tcgcaagcaa
ggctgacaag actttggccc aaacatttgt acgcggtgga caacaggagc 4080
cacccatcgt ctgtcacggg ctagccggtc gtgcgtcctg tcaggctcca cctaggctcc
4140 atgccactcc atacaatccc actagtgtac cgctaggccg cttttagctc
ccatctaaga 4200 cccccccaaa acctccactg tacagtgcac tgtactgtgt
ggcgatcaag ggcaagggaa 4260 aaaaggcgca aacatgcacg catggaatga
cgtaggtaag gcgttactag actgaaaagt 4320 ggcacatttc ggcgtgccaa
agggtcctag gtgcgtttcg cgagctgggc gccaggccaa 4380 gccgctccaa
aacgcctctc cgactccctc cagcggcctc catatcccca tccctctcca 4440
cagcaatgtt gttaagcctt gcaaacgaaa aaatagaaag gctaataagc ttccaatatt
4500 gtggtgtacg ctgcataacg caacaatgag cgccaaacaa cacacacaca
cagcacacag 4560 cagcattaac cacgatgttt aaacagtgta cgcagatccc
gtcaacagtt ttatatatcg 4620 tagttacaac catcaacact ttttggtaag
tgtaccattc tatactccaa ctggtctgca 4680 actgtacaag tagacatgtt
aatggtagtt aataacatct acagcagaac ctatggtaaa 4740 gacattgcat
ttttacagga agtatcgtcc tacacgttga taaatccaaa gatgcggaac 4800
ttcttccact tttatcatca tcccctactc gtacactcgt actctttgtt cgatcgcgat
4860 tcatttctat aaataatctt gtatgtacat gcggccgcgc ctacttaagc
aacgggcttg 4920 ataacagcgg ggggggtgcc cacgttgttg cggttgcgga
agaacagaac acccttacca 4980 gcaccctcgg caccagcgct gggctcaacc
cactggcaca tacgcgcact gcggtacatg 5040 gcgcggatga agccacgagg
accatcctgg acatcagccc ggtagtgctt gcccatgatg 5100 ggcttaatgg
cctcggtggc ctcgtccgcg ttgtagaagg ggatgctgct gacgtagtgg 5160
tggaggacat gagtctcgat gatgccgtgg agaaggtggc ggccgatgaa gcccatctca
5220 cggtcaatgg tagcagcggc accacggacg aagttccact cgtcgttggt
gtagtgggga 5280 agggtagggt cggtgtgctg gaggaaggtg atggcaacga
gccagtggtt aacccagagg 5340 tagggaacaa agtaccagat ggccatgttg
tagaaaccga acttctgaac gaggaagtac 5400 agagcagtgg ccatcagacc
gataccaata tcgctgagga cgatgagctt agcgtcactg 5460 ttctcgtaca
gagggctgcg gggatcgaag tggttaacac caccgccgag gccgttatgc 5520
ttgcccttgc cgcgaccctc acgctggcgc tcgtggtagt tgtggccggt aacattggtg
5580 atgaggtagt tgggccagcc nacgannnnc tcagtaagat gagcgagctc
gtgggtcatc 5640 tttccgagac gagtagcctg ctgctcgcgg gttcggggaa
cgaagaccat gtcacgctcc 5700 atgttgccag tggccttgtg gtgctttcgg
tgggagattt gccagctgaa gtaggggaca 5760 aggagggaag agtgaagaac
ccagccagta atgtcgttga tgatgcgaga atcggagaaa 5820 gcaccgtgac
cgcactcatg ggcaataacc cagagaccag taccgaaaag accctgaaga 5880
acggtgtaca cggcccacag accagcgcgg gcgggggtgg aggggatata ttcgggggtc
5940 acaaagttgt accagatgct gaaagtggta gtcaggagga caatgtcgcg
gaggatataa 6000 ccgtatccct tgagagcgga gcgcttgaag cagtgcttag
ggatggcatt gtagatgtcc 6060 ttgatggtaa agtcgggaac ctcgaactgg
ttgccgtagg tgtcgagcat gacaccatac 6120 tcggacttgg gcttggcgat
atcaacctcg gacatggacg agagcgatgt ggaagaggcc 6180 gagtggcggg
gagagtctga aggagagacg gcggcagact cagaatccgt cacagtagtt 6240
gaggtgacgg tgcgtctaag cgcagggttc tgcttgggca gagccgaagt ggacgccatg
6300 gttgtgaatt agggtggtga gaatggttgg ttgtagggaa gaatcaaagg
ccggtctcgg 6360 gatccgtggg tatatatata tatatatata tatacgatcc
ttcgttacct ccctgttctc 6420 aaaactgtgg tttttcgttt ttcgtttttt
gctttttttg atttttttag ggccaactaa 6480 gcttccagat ttcgctaatc
acctttgtac taattacaag aaaggaagaa gctgattaga 6540 gttgggcttt
ttatgcaact gtgctactcc ttatctctga tatgaaagtg tagacccaat 6600
cacatcatgt catttagagt tggtaatact gggaggatag ataaggcacg aaaacgagcc
6660 atagcagaca tgctgggtgt agccaagcag aagaaagtag atgggagcca
attgacgagc 6720 gagggagcta cgccaatccg acatacgaca cgctgagatc
gtcttggccg gggggtacct 6780 acagatgtcc aagggtaagt gcttgactgt
aattgtatgt ctgaggacaa atatgtagtc 6840 agccgtataa agtcatacca
ggcaccagtg ccatcatcga accactaact ctctatgata 6900 catgcctccg
gtattattgt accatgcgtc gctttgttac atacgtatct tgcctttttc 6960
tctcagaaac tccagacttt ggctattggt cgagataagc ccggaccata gtgagtcttt
7020 cacactctac atttctccct tgctccaact atttaaattg ccccggagaa
gacggccagg 7080 ccgcctagat gacaaattca acaactcaca gctgactttc
tgccattgcc actagggggg 7140 ggccttttta tatggccaag ccaagctctc
cacgtcggtt gggctgcacc caacaataaa 7200 tgggtagggt tgcaccaaca
aagggatggg atggggggta gaagatacga ggataacggg 7260 gctcaatggc
acaaataaga acgaatactg ccattaagac tcgtgatcca gcgactgaca 7320
ccattgcatc atctaagggc ctcaaaacta cctcggaact gctgcgctga tctggacacc
7380 acagaggttc cgagcacttt aggttgcacc aaatgtccca ccaggtgcag
gcagaaaacg 7440 ctggaacagc gtgtacagtt tgtcttaaca aaaagtgagg
gcgctgaggt cgagcagggt 7500 ggtgtgactt gttatagcct ttagagctgc
gaaagcgcgt atggatttgg ctcatcaggc 7560 cagattgagg gtctgtggac
acatgtcatg ttagtgtact tcaatcgccc cctggatata 7620 gccccgacaa
taggccgtgg cctcattttt ttgccttccg cacatttcca ttgctcggta 7680
cccacacctt gcttctcctg cacttgccaa ccttaatact ggtttacatt gaccaacatc
7740 ttacaagcgg ggggcttgtc tagggtatat ataaacagtg gctctcccaa
tcggttgcca 7800 gtctcttttt tcctttcttt ccccacagat tcgaaatcta
aactacacat cacagaattc 7860 cgagccgtga gtatccacga caagatcagt
gtcgagacga cgcgttttgt gtaatgacac 7920 aatccgaaag tcgctagcaa
cacacactct ctacacaaac taacccagct ctggtaccat 7980 ggtgaaggct
tctcgacagg ctctgcccct cgtcatcgac ggaaaggtgt acgacgtctc 8040
cgcttgggtg aacttccacc ctggtggagc tgaaatcatt gagaactacc agggacgaga
8100 tgctactgac gccttcatgg ttatgcactc tcaggaagcc ttcgacaagc
tcaagcgaat 8160 gcccaagatc aaccaggctt ccgagctgcc tccccaggct
gccgtcaacg aagctcagga 8220 ggatttccga aagctccgag aagagctgat
cgccactggc atgtttgacg cctctcccct 8280 ctggtactcg tacaagatct
tgaccaccct gggtcttggc gtgcttgcct tcttcatgct 8340 ggtccagtac
cacctgtact tcattggtgc tctcgtgctc ggtatgcact accagcaaat 8400
gggatggctg tctcatgaca tctgccacca ccagaccttc aagaaccgaa actggaataa
8460 cgtcctgggt ctggtctttg gcaacggact ccagggcttc tccgtgacct
ggtggaagga 8520 cagacacaac gcccatcatt ctgctaccaa cgttcagggt
cacgatcccg acattgataa 8580 cctgcctctg ctcgcctggt ccgaggacga
tgtcactcga gcttctccca tctcccgaaa 8640 gctcattcag ttccaacagt
actatttcct ggtcatctgt attctcctgc gattcatctg 8700 gtgtttccag
tctgtgctga ccgttcgatc cctcaaggac cgagacaacc agttctaccg 8760
atctcagtac aagaaagagg ccattggact cgctctgcac tggactctca agaccctgtt
8820 ccacctcttc tttatgccct ccatcctgac ctcgatgctg gtgttctttg
tttccgagct 8880 cgtcggtggc ttcggaattg ccatcgtggt cttcatgaac
cactaccctc tggagaagat 8940 cggtgattcc gtctgggacg gacatggctt
ctctgtgggt cagatccatg agaccatgaa 9000 cattcgacga ggcatcatta
ctgactggtt ctttggaggc ctgaactacc agatcgagca 9060 ccatctctgg
cccaccctgc ctcgacacaa cctcactgcc gtttcctacc aggtggaaca 9120
gctgtgccag aagcacaacc tcccctaccg aaaccctctg ccccatgaag gtctcgtcat
9180 cctgctccga tacctgtccc agttcgctcg aatggccgag aagcagcccg
gtgccaaggc 9240 tcagtaagcg gccgcaagtg tggatgggga agtgagtgcc
cggttctgtg tgcacaattg 9300 gcaatccaag atggatggat tcaacacagg
gatatagcga gctacgtggt ggtgcgagga 9360 tatagcaacg gatatttatg
tttgacactt gagaatgtac gatacaagca ctgtccaagt 9420 acaatactaa
acatactgta catactcata ctcgtacccg ggcaacggtt tcacttgagt 9480
gcagtggcta gtgctcttac tcgtacagtg tgcaatactg cgtatcatag tctttgatgt
9540 atatcgtatt cattcatgtt agttgcgtac gggtgaagct tccactggtc
ggcgtggtag 9600 tggggcagag tggggtcggt gtgctgcagg taggtgatgg
ccacgagcca gtggttgacc 9660 cacaggtagg ggatcaggta gtagagggtg
acggaagcca ggccccatcg gttgatggag 9720 tatgcgatga cggacatggt
gataccaata ccgacgttag agatccagat gttgaaccag 9780 tccttcttct
caaacagcgg ggcgttgggg ttgaagtggt tgacagccca tttgttgagc 9840
ttggggtact tctgtccggt aacgtaagac agcagataca gaggccatcc aaacacctgc
9900 tgggtgatga ggccgtagag ggtcatgagg ggagcgtcct cagcaagctc
agaccagtca 9960 tgggcgcctc ggttctccat aaactccttt cggtccttgg
gcacaaacac catatcacgg 10020 gtgaggtgac cagtggactt gtggtgcatg
gagtgggtca gcttccaggc gtagtaaggg 10080 accagcatgg aggagtgcag
aacccatccg gtgacgttgt tgacggtgtt agagtcggag 10140 aaagcagagt
ggccacactc gtgggcaaga acccacagac cggtgccaaa cagaccctgg 10200
acaatggagt acatggccca ggccacagct cggccggaag ccgagggaat aagaggcagg
10260 tacgcgtagg ccatgtaggc aaaaacggcg ataaagaagc aggcgcgcca
gctgcattaa 10320 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
ggcgctcttc cgcttcctcg 10380 ctcactgact cgctgcgctc ggtcgttcgg
ctgcggcgag cggtatcagc tcactcaaag 10440 gcggtaatac ggttatccac
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 10500 ggccagcaaa
aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 10560
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
10620 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
tcctgttccg 10680 accctgccgc ttaccggata cctgtccgcc tttctccctt
cgggaagcgt ggcgctttct 10740 catagctcac gctgtaggta tctcagttcg
gtgtaggtcg ttcgctccaa gctgggctgt 10800 gtgcacgaac cccccgttca
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 10860 tccaacccgg
taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 10920
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
10980 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
cggaaaaaga 11040 gttggtagct cttgatccgg caaacaaacc accgctggta
gcggtggttt ttttgtttgc 11100 aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag atcctttgat cttttctacg 11160 gggtctgacg ctcagtggaa
cgaaaactca cgttaaggga ttttggtcat gagattatca 11220 aaaaggatct
tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 11280
atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
11340 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
gataactacg 11400 atacgggagg gcttaccatc tggccccagt gctgcaatga
taccgcgaga cccacgctca 11460 ccggctccag atttatcagc aataaaccag
ccagccggaa gggccgagcg cagaagtggt 11520 cctgcaactt tatccgcctc
catccagtct attaattgtt gccgggaagc tagagtaagt 11580 agttcgccag
ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 11640
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
11700 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
cgttgtcaga 11760 agtaagttgg ccgcagtgtt atcactcatg gttatggcag
cactgcataa ttctcttact 11820 gtcatgccat ccgtaagatg cttttctgtg
actggtgagt actcaaccaa gtcattctga 11880 gaatagtgta tgcggcgacc
gagttgctct tgcccggcgt caatacggga taataccgcg 11940 ccacatagca
gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 12000
tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
12060 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
aaggcaaaat 12120 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
tactcatact cttccttttt 12180 caatattatt gaagcattta tcagggttat
tgtctcatga gcggatacat atttgaatgt 12240 atttagaaaa ataaacaaat
aggggttccg cgcacatttc cccgaaaagt gccacctgat 12300 gcggtgtgaa
ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc 12360
gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
12420 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
agggttgagt 12480 gttgttccag tttggaacaa gagtccacta ttaaagaacg
tggactccaa cgtcaaaggg 12540 cgaaaaaccg tctatcaggg cgatggccca
ctacgtgaac catcacccta atcaagtttt 12600 ttggggtcga ggtgccgtaa
agcactaaat cggaacccta aagggagccc ccgatttaga 12660 gcttgacggg
gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 12720
ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
12780 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
tgttgggaag 12840 ggcgatcggt gcgggcctct tcgctattac gccagctggc
gaaaggggga tgtgctgcaa 12900 ggcgattaag ttgggtaacg ccagggtttt
cccagtcacg acgttgtaaa acgacggcca 12960 gtgaattgta atacgactca
ctatagggcg aattgggccc gacgtcgcat gcttgaatct 13020 acaagtagga
gggttggagt gattaagtga aacttcttta acggctctat gccagttcta 13080
ttgatatccg aaacatcagt atgaaggtct gataagggtg acttcttccc acagattcgt
13140 atcagtacga gtacgagacc ggtacttgta acagtattga tactaaaggg
aaactacaac 13200 ggttgtcagc gtaatgtgac ttcgcccatg aacgcagaca
cgcagtgccg agtgcggtga 13260 tatcgcctac tcgttacgtc catggactac
acaacccctc ggcttcgctt ggcttagcct 13320 cgggctcggt gctgttcagt
taaaacacaa tcaaataaca tttctacttt ttagaaggca 13380 ggccgtcagg
agcaactccg actccattga cgtttctaaa catctgaatg ccttccttac 13440
cttcaacaaa ctggcaggtt cgggcgacag tgtaaagaga cttgatgaag ttggtgtcgt
13500 cgtgtcggta gtgcttgccc atgaccttct tgatcttctc agtggcgatt
cgggcgttgt 13560 agaagggaat tcctttacct gcaggataac ttcgtataat
gtatgctata cgaagttatg 13620 atctctctct tgagcttttc cataacaagt
tcttctgcct ccaggaagtc catgggtggt 13680 ttgatcatgg ttttggtgta
gtggtagtgc agtggtggta ttgtgactgg ggatgtagtt 13740 gagaataagt
catacacaag tcagctttct tcgagcctca tataagtata agtagttcaa 13800
cgtattagca ctgtacccag catctccgta tcgagaaaca caacaacatg ccccattgga
13860 cagatcatgc ggatacacag gttgtgcagt atcatacata ctcgatcaga
caggtcgtct 13920 gaccatcata caagctgaac aagcgctcca tacttgcacg
ctctctatat acacagttaa 13980 attacatatc catagtctaa cctctaacag
ttaatcttct ggtaagcctc ccagccagcc 14040 ttctggtatc gcttggcctc
ctcaatagga tctcggttct ggccgtacag acctcggccg 14100 acaattatga
tatccgttcc ggtagacatg acatcctcaa cagttcggta ctgctgtccg 14160
agagcgtctc ccttgtcgtc aagacccacc ccgggggtca gaataagcca gtcctcagag
14220 tcgcccttag gtcggttctg ggcaatgaag ccaaccacaa actcggggtc
ggatcgggca 14280 agctcaatgg tctgcttgga gtactcgcca gtggccagag
agcccttgca agacagctcg 14340 gccagcatga gcagacctct ggccagcttc
tcgttgggag aggggactag gaactccttg 14400 tactgggagt tctcgtagtc
agagacgtcc tccttcttct gttcagagac agtttcctcg 14460 gcaccagctc
gcaggccagc aatgattccg gttccgggta caccgtgggc gttggtgata 14520
tcggaccact cggcgattcg gtgacaccgg tactggtgct tgacagtgtt gccaatatct
14580 gcgaactttc tgtcctcgaa caggaagaaa ccgtgcttaa gagcaagttc
cttgaggggg 14640 agcacagtgc cggcgtaggt gaagtcgtca atgatgtcga
tatgggtttt gatcatgcac 14700 acataaggtc cgaccttatc ggcaagctca
atgagctcct tggtggtggt aacatccaga 14760 gaagcacaca ggttggtttt
cttggctgcc acgagcttga gcactcgagc ggcaaaggcg 14820 gacttgtgga
cgttagctcg agcttcgtag gagggcattt tggtggtgaa gaggagactg 14880
aaataaattt agtctgcaga actttttatc ggaaccttat ctggggcagt gaagtatatg
14940 ttatggtaat agttacgagt tagttgaact tatagataga ctggactata
cggctatcgg 15000 tccaaattag aaagaacgtc aatggctctc tgggcgtcgc
ctttgccgac aaaaatgtga 15060 tcatgatgaa agccagcaat gacgttgcag
ctgatattgt tgtcggccaa ccgcgccgaa 15120 aacgcagctg tcagacccac
agcctccaac gaagaatgta tcgtcaaagt gatccaagca 15180 cactcatagt
tggagtcgta ctccaaaggc ggcaatgacg agtcagacag atactcgtcg 15240
acgcgataac ttcgtataat gtatgctata cgaagttatc gtacgatagt tagtagacaa
15300 caat 15304 <210> SEQ ID NO 71 <211> LENGTH: 1272
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: mutant EgD8S-23
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(1270) <223> OTHER INFORMATION: mutant
EgD8S-23 delta-8 desaturase CDS <400> SEQUENCE: 71 catggtgaag
gcttctcgac aggctctgcc cctcgtcatc gacggaaagg tgtacgacgt 60
ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg
120 agatgctact gacgccttca tggttatgca ctctcaggaa gccttcgaca
agctcaagcg 180 aatgcccaag atcaaccagg cttccgagct gcctccccag
gctgccgtca acgaagctca 240 ggaggatttc cgaaagctcc gagaagagct
gatcgccact ggcatgtttg acgcctctcc 300 cctctggtac tcgtacaaga
tcttgaccac cctgggtctt ggcgtgcttg ccttcttcat 360 gctggtccag
taccacctgt acttcattgg tgctctcgtg ctcggtatgc actaccagca 420
aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa
480 taacgtcctg ggtctggtct ttggcaacgg actccagggc ttctccgtga
cctggtggaa 540 ggacagacac aacgcccatc attctgctac caacgttcag
ggtcacgatc ccgacattga 600 taacctgcct ctgctcgcct ggtccgagga
cgatgtcact cgagcttctc ccatctcccg 660 aaagctcatt cagttccaac
agtactattt cctggtcatc tgtattctcc tgcgattcat 720 ctggtgtttc
cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta 780
ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagaccct
840 gttccacctc ttctttatgc cctccatcct gacctcgatg ctggtgttct
ttgtttccga 900 gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg
aaccactacc ctctggagaa 960 gatcggtgat tccgtctggg acggacatgg
cttctctgtg ggtcagatcc atgagaccat 1020 gaacattcga cgaggcatca
ttactgactg gttctttgga ggcctgaact accagatcga 1080 gcaccatctc
tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga 1140
acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt
1200 catcctgctc cgatacctgt cccagttcgc tcgaatggcc gagaagcagc
ccggtgccaa 1260 ggctcagtaa gc 1272 <210> SEQ ID NO 72
<211> LENGTH: 422 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: mutant EgD8S-23, comprising M1, M2, M3, M8, M12, M15,
M16, M18, M21, M26, M45, M46, M68 and M70 mutation sites
<400> SEQUENCE: 72 Met Val Lys Ala Ser Arg Gln Ala Leu Pro
Leu Val Ile Asp Gly Lys 1 5 10 15 Val Tyr Asp Val Ser Ala Trp Val
Asn Phe His Pro Gly Gly Ala Glu 20 25 30 Ile Ile Glu Asn Tyr Gln
Gly Arg Asp Ala Thr Asp Ala Phe Met Val 35 40 45 Met His Ser Gln
Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 50 55 60 Asn Gln
Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 65 70 75 80
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 85
90 95 Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu
Gly 100 105 110 Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His
Leu Tyr Phe 115 120 125 Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln
Gln Met Gly Trp Leu 130 135 140 Ser His Asp Ile Cys His His Gln Thr
Phe Lys Asn Arg Asn Trp Asn 145 150 155 160 Asn Val Leu Gly Leu Val
Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 165 170 175 Thr Trp Trp Lys
Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 180 185 190 Gln Gly
His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 195 200 205
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 210
215 220 Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe
Ile 225 230 235 240 Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu
Lys Asp Arg Asp 245 250 255 Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys
Glu Ala Ile Gly Leu Ala 260 265 270 Leu His Trp Thr Leu Lys Thr Leu
Phe His Leu Phe Phe Met Pro Ser 275 280 285 Ile Leu Thr Ser Met Leu
Val Phe Phe Val Ser Glu Leu Val Gly Gly 290 295 300 Phe Gly Ile Ala
Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 305 310 315 320 Ile
Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 325 330
335 His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe
340 345 350 Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr
Leu Pro 355 360 365 Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu
Gln Leu Cys Gln 370 375 380 Lys His Asn Leu Pro Tyr Arg Asn Pro Leu
Pro His Glu Gly Leu Val 385 390 395 400 Ile Leu Leu Arg Tyr Leu Ser
Gln Phe Ala Arg Met Ala Glu Lys Gln 405 410 415 Pro Gly Ala Lys Ala
Gln 420 <210> SEQ ID NO 73 <211> LENGTH: 777
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <223>
OTHER INFORMATION: delta-9 elongase <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-9 ELONGASES AND THEIR USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: WO 2007/061742 <311> PATENT FILING DATE: 2006-11-16
<312> PUBLICATION DATE: 2007-05-31 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(777) <400> SEQUENCE: 73
atggaggtgg tgaatgaaat agtctcaatt gggcaggaag ttttacccaa agttgattat
60 gcccaactct ggagtgatgc cagtcactgt gaggtgcttt acttgtccat
cgcatttgtc 120 atcttgaagt tcactcttgg cccccttggt ccaaaaggtc
agtctcgtat gaagtttgtt 180 ttcaccaatt acaaccttct catgtccatt
tattcgttgg gatcattcct ctcaatggca 240 tatgccatgt acaccatcgg
tgttatgtct gacaactgcg agaaggcttt tgacaacaac 300 gtcttcagga
tcaccacgca gttgttctat ttgagcaagt tcctggagta tattgactcc 360
ttctatttgc cactgatggg caagcctctg acctggttgc aattcttcca tcatttgggg
420 gcaccgatgg atatgtggct gttctataat taccgaaatg aagctgtttg
gatttttgtg 480 ctgttgaatg gtttcatcca ctggatcatg tacggttatt
attggaccag attgatcaag 540 ctgaagttcc ccatgccaaa atccctgatt
acatcaatgc agatcattca attcaatgtt 600 ggtttctaca ttgtctggaa
gtacaggaac attccctgtt atcgccaaga tgggatgagg 660 atgtttggct
ggttcttcaa ttacttttat gttggcacag tcttgtgttt gttcttgaat 720
ttctatgtgc aaacgtatat cgtcaggaag cacaagggag ccaaaaagat tcagtga 777
<210> SEQ ID NO 74 <211> LENGTH: 13707 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pZKSL-555R
<400> SEQUENCE: 74 aaacagtgta cgcagatctg cccatgatgg
gggctcccac caccagcaat cagggccctg 60 attacacacc cacctgtaat
gtcatgctgt tcatcgtggt taatgctgct gtgtgctgtg 120 tgtgtgtgtt
gtttggcgct cattgttgcg ttatgcagcg tacaccacaa tattggaagc 180
ttattagcct ttctattttt tcgtttgcaa ggcttaacaa cattgctgtg gagagggatg
240 gggatatgga ggccgctgga gggagtcgga gaggcgtttt ggagcggctt
ggcctggcgc 300 ccagctcgcg aaacgcacct aggacccttt ggcacgccga
aatgtgccac ttttcagtct 360 agtaacgcct tacctacgtc attccatgcg
tgcatgtttg cgcctttttt cccttgccct 420 tgatcgccac acagtacagt
gcactgtaca gtggaggttt tgggggggtc ttagatggga 480 gctaaaagcg
gcctagcggt acactagtgg gattgtatgg agtggcatgg agcctaggtg 540
gagcctgaca ggacgcacga ccggctagcc cgtgacagac gatgggtggc tcctgttgtc
600 caccgcgtac aaatgtttgg gccaaagtct tgtcagcctt gcttgcgaac
ctaattccca 660 attttgtcac ttcgcacccc cattgatcga gccctaaccc
ctgcccatca ggcaatccaa 720 ttaagctcgc attgtctgcc ttgtttagtt
tggctcctgc ccgtttcggc gtccacttgc 780 acaaacacaa acaagcatta
tatataaggc tcgtctctcc ctcccaacca cactcacttt 840 tttgcccgtc
ttcccttgct aacacaaaag tcaagaacac aaacaaccac cccaaccccc 900
ttacacacaa gacatatcta cagcaatggc catggctctc tcccttacta ccgagcagct
960 gctcgagcga cccgacctgg ttgccatcga cggcattctc tacgatctgg
aaggtcttgc 1020 caaggtccat cccggaggcg acttgatcct cgcttctggt
gcctccgatg cttctcctct 1080 gttctactcc atgcaccctt acgtcaagcc
cgagaactcg aagctgcttc aacagttcgt 1140 gcgaggcaag cacgaccgaa
cctccaagga cattgtctac acctacgact ctccctttgc 1200 acaggacgtc
aagcgaacta tgcgagaggt catgaaaggt cggaactggt atgccacacc 1260
tggattctgg ctgcgaaccg ttggcatcat tgctgtcacc gccttttgcg agtggcactg
1320 ggctactacc ggaatggtgc tgtggggtct cttgactgga ttcatgcaca
tgcagatcgg 1380 cctgtccatt cagcacgatg cctctcatgg tgccatcagc
aaaaagccct gggtcaacgc 1440 tctctttgcc tacggcatcg acgtcattgg
atcgtccaga tggatctggc tgcagtctca 1500 catcatgcga catcacacct
acaccaatca gcatggtctc gacctggatg ccgagtccgc 1560 agaaccattc
cttgtgttcc acaactaccc tgctgccaac actgctcgaa agtggtttca 1620
ccgattccag gcctggtaca tgtacctcgt gcttggagcc tacggcgttt cgctggtgta
1680 caaccctctc tacatcttcc gaatgcagca caacgacacc attcccgagt
ctgtcacagc 1740 catgcgagag aacggctttc tgcgacggta ccgaaccctt
gcattcgtta tgcgagcttt 1800 cttcatcttt cgaaccgcct tcttgccctg
gtatctcact ggaacctccc tgctcatcac 1860 cattcctctg gtgcccactg
ctaccggtgc cttcctcacc ttctttttca tcttgtctca 1920 caacttcgat
ggctcggagc gaatccccga caagaactgc aaggtcaaga gctccgagaa 1980
ggacgttgaa gccgatcaga tcgactggta cagagctcag gtggagacct cttccaccta
2040 cggtggaccc attgccatgt tctttactgg cggtctcaac ttccagatcg
agcatcacct 2100 ctttcctcga atgtcgtctt ggcactatcc cttcgtgcag
caagctgtcc gagagtgttg 2160 cgaacgacac ggagttcggt acgtcttcta
ccctaccatt gtgggcaaca tcatttccac 2220 cctcaagtac atgcacaaag
tcggtgtggt tcactgtgtc aaggacgctc aggattccta 2280 agcggccgca
agtgtggatg gggaagtgag tgcccggttc tgtgtgcaca attggcaatc 2340
caagatggat ggattcaaca cagggatata gcgagctacg tggtggtgcg aggatatagc
2400 aacggatatt tatgtttgac acttgagaat gtacgataca agcactgtcc
aagtacaata 2460 ctaaacatac tgtacatact catactcgta cccgggcaac
ggtttcactt gagtgcagtg 2520 gctagtgctc ttactcgtac agtgtgcaat
actgcgtatc atagtctttg atgtatatcg 2580 tattcattca tgttagttgc
gtacgctgtg ttgttgtatg tggtgaagct tgacaatgga 2640 tggtgtgtcg
tatcaggctg gggaacaatt gtgcttaagt atgctgcagt tgagtaagag 2700
tcatcgctcc accaaaataa agtttgccat tagggttgga gagagagatg gtggctggaa
2760 gaattaaatg acatcaagct gaggattgtg ggtgtgcaat aacacatgtt
aggggtgacc 2820 tgtggctcga aatctgataa ttattttgta actttatgat
tattcttaga ttttttaata 2880 ttcctctata taacacataa gtagctgtcg
tctagttgtt catagcctga ctcctgcaat 2940 agattagtgc agagtgattt
tgtgcaattg agagccacgg ttgagtcaag tgactttgtg 3000 tgtgaagtca
tcttacgttt caagtctcac aggttactca attggttggt tgtctgccct 3060
ttacagatat ttacagtacc tgagcgtaaa gtcgttcatc cacggaatga ctgttcctgt
3120 cacgcagtca tgatcatgga tgtggctggt caggaaccat tttggatagg
agacttaggg 3180 attggactat tattgaaaaa actgagccga atatgatata
gttctatttg aatgcagaac 3240 ttctgatggt caattcactt atttcaggca
tatcggtcat ggtggcagct gccacgatgt 3300 tatctcgttg gaaacctcgg
cgcgccagct gcattaatga atcggccaac gcgcggggag 3360 aggcggtttg
cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 3420
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
3480 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
ccaggaaccg 3540 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
ccccctgacg agcatcacaa 3600 aaatcgacgc tcaagtcaga ggtggcgaaa
cccgacagga ctataaagat accaggcgtt 3660 tccccctgga agctccctcg
tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3720 gtccgccttt
ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3780
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
3840 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
gacacgactt 3900 atcgccactg gcagcagcca ctggtaacag gattagcaga
gcgaggtatg taggcggtgc 3960 tacagagttc ttgaagtggt ggcctaacta
cggctacact agaagaacag tatttggtat 4020 ctgcgctctg ctgaagccag
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4080 acaaaccacc
gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4140
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
4200 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
cctagatcct 4260 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
tatgagtaaa cttggtctga 4320 cagttaccaa tgcttaatca gtgaggcacc
tatctcagcg atctgtctat ttcgttcatc 4380 catagttgcc tgactccccg
tcgtgtagat aactacgata cgggagggct taccatctgg 4440 ccccagtgct
gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4500
aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
4560 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
atagtttgcg 4620 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
tcgtcgtttg gtatggcttc 4680 attcagctcc ggttcccaac gatcaaggcg
agttacatga tcccccatgt tgtgcaaaaa 4740 agcggttagc tccttcggtc
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4800 actcatggtt
atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4860
ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
4920 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
ctttaaaagt 4980 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
aggatcttac cgctgttgag 5040 atccagttcg atgtaaccca ctcgtgcacc
caactgatct tcagcatctt ttactttcac 5100 cagcgtttct gggtgagcaa
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 5160 gacacggaaa
tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 5220
gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
5280 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata
ccgcacagat 5340 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
aatattttgt taaaattcgc 5400 gttaaatttt tgttaaatca gctcattttt
taaccaatag gccgaaatcg gcaaaatccc 5460 ttataaatca aaagaataga
ccgagatagg gttgagtgtt gttccagttt ggaacaagag 5520 tccactatta
aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 5580
tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
5640 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
agccggcgaa 5700 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
gctagggcgc tggcaagtgt 5760 agcggtcacg ctgcgcgtaa ccaccacacc
cgccgcgctt aatgcgccgc tacagggcgc 5820 gtccattcgc cattcaggct
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 5880 ctattacgcc
agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 5940
gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
6000 tagggcgaat tgggcccgac gtcgcatgca ttccatagcc acacctttgc
ctatggcttc 6060 acaaccgaag gcaattcgag aggtcgcgct tatggaatcg
actcgtataa agctgaaggg 6120 aaagggagac gttccgagcg ctcagatgca
atagtcgtcc agctaatgtg gattcaaaaa 6180 caaccccaac agtaatcttg
aaaatttgaa cggatcaatc tgaacactct tgctccaggt 6240 cattcttcta
acgcacatcc ccagagtcta gagggagttg tgttgtgaac atcctaataa 6300
acaatgcaat ggattcggga tatcttctgt ctcgccccct actcgatgtc gagtaaaccg
6360 atcaccaact aacaatactc ctccgcgttc tgccattgac tctcaaacag
acatcgctat 6420 caacggaaca gcatatttta gcttcttagg acaataaata
ttgataatgc cggctctccc 6480 tcggtatatt aagcaatcca ttcatacact
cattcatcag gttaatttta tatatataat 6540 ttgtctattc aaacaccgta
aattactggt accatcatct cctccttttc aaatacacgt 6600 ctatttgcat
taatgaaatt actcgccaat tcgcagaacg tgtttgtcga acagagcctt 6660
agctcgggtc cagacaggag cagtgtctcg ctgaggaagc tgcaggagag ttaattaact
6720 cacctgcagg attgagacta tgaatggatt cccgtgcccg tattactcta
ctaatttgat 6780 cttggaacgc gaaaatacgt ttctaggact ccaaagaatc
tcaactcttg tccttactaa 6840 atatactacc catagttgat ggtttacttg
aacagagagg acatgttcac ttgacccaaa 6900 gtttctcgca tctcttggat
atttgaacaa cggcgtccac tgaccgtcag ttatccagtc 6960 acaaaacccc
cacattcata cattcccatg tacgtttaca aagttctcaa ttccatcgtg 7020
caaatcaaaa tcacatctat tcattcatca tatataaacc catcatgtct actaacactc
7080 acaactccat agaaaacatc gactcagaac acacgctcca tgcggccgct
taggaatcct 7140 gtgcgtcctt cacgcagtgg acgacaccca ccttatgcat
gtacttcagg gtggagatga 7200 tgttgccgac gatggtaggg tagaaaacat
atcgcactcc atgtcgttcg caacactccc 7260 ggaccgcctg ctggacgaag
gggtagtgcc aagacgacat ccggggaaag aggtggtgct 7320 cgatctggaa
attgagaccg ccagtgaaga acatggcgat ggggccaccg tatgtggagg 7380
acgtctccac ctgcgcccga taccagtcaa tttggtcagc ctcaacgtcc ttctcagatc
7440 gcttaacctt gcagttcttg tcggggatcc gttcggagcc atcaaaattg
tgggacaaaa 7500 tgaagaagaa cgtcaagaag gcaccagttg cggtgggcac
cagaggaatg gtgatcagca 7560 atgaggtccc agtgaggtac cagggcaaga
atgcggtccg gaagatgaag aaagctcgca 7620 tcacgaatgc aagtgtgcgg
tagcgccgca gaaagccatt ttcccgcatg gccgtgacag 7680 actctgggat
ggtgtcattg tgctgcatcc ggaaaatgta gagcgggttg tacaccagcg 7740
ataccccgta tgcccccagc acaaggtaca tgtaccaagc ctggaagcgg tggaaccact
7800 ttcgggcggt gtttgcggcg gggtagttgt ggaacaccag gaacggctct
gccgactccg 7860 catccaggtc gaggccgtgc tggttggtgt aggtgtggtg
ccgcatgatg tgcgactgca 7920 gccaaatcca ccgggacgat ccgatgacgt
caatgccgta ggcgaagagg gcgttgaccc 7980 aaggcttctt gctgatggcc
ccgtgggacg catcatgctg gatggataag ccgatctgca 8040 tgtgcatgaa
tccagtcaac aggccccaca gcaccatccc cgtggtagcc cagtgccact 8100
cgcaaaaggc cgtcacggcg atgatcccaa cggtgcgcag ccagaagcca ggggttgcgt
8160 accagttcct ccctttcatc acctcgcgca ttgtccgctt aacgtcttgt
gcgaagggag 8220 aatcatacgt gtagacaatg tccttcgagg tgcggtcatg
cttccctcgg acgaactgtt 8280 gaagcaattt ggagttctcc ggtttgacgt
atggatgcat tgaataaaag agaggggagg 8340 catcagaggc accagaagcg
agaatcaaat ctcctcctgg atgaactttg gcaagccctt 8400 caaggtcgta
gaggatgcca tcaatcgcaa ccaaatcagg gcgttctaac agctgttctg 8460
tggtaagact gagagccatg gagagctggg ttagtttgtg tagagagtgt gtgttgctag
8520 cgactttcgg attgtgtcat tacacaaaac gcgtcgtctc gacactgatc
ttgtcgtgga 8580 tactcacggc tcggacatcg tcgccgacga tgacaccgga
ctttcgctta aggacgtcag 8640 taacaggcat tgtgtgatgt gtagtttaga
tttcgaatct gtggggaaag aaaggaaaaa 8700 agagactggc aaccgattgg
gagagccact gtttatatat accctagaca agccccccgc 8760 ttgtaagatg
ttggtcaatg taaaccagta ttaaggttgg caagtgcagg agaagcaagg 8820
tgtgggtacc gagcaatgga aatgtgcgga aggcaaaaaa atgaggccac ggcctattgt
8880 cggggctata tccagggggc gattgaagta cactaacatg acatgtgtcc
acagaccctc 8940 aatctggcct gatgagccaa atccatacgc gctttcgcag
ctctaaaggc tataacaagt 9000 cacaccaccc tgctcgacct cagcgccctc
actttttgtt aagacaaact gtacacgctg 9060 ttccagcgtt ttctgcctgc
acctggtggg acatttggtg caacctaaag tgctcggaac 9120 ctctgtggtg
tccagatcag cgcagcagtt ccgaggtagt tttgaggccc ttagatgatg 9180
caatggtgtc agtcgctgga tcacgagtct taatggcagt attcgttctt atttgtgcca
9240 ttgagccccg ttatcctcgt atcttctacc ccccatccca tccctttgtt
ggtgcaaccc 9300 tacccattta ttgttgggtg cagcccaacc gacgtggaga
gcttggcttg gccatataaa 9360 aaggcccccc cctagtggca atggcagaaa
gtcagctgtg agttgttgaa tttgtcatct 9420 aggcggcctg gccgtcttct
ccggggcaat tggggctgtt ttttgggaca caaatacgcc 9480 gccaacccgg
tctctcctga attccgtcgt cgcctgagtc gacatcattt atttaccagt 9540
tggccacaaa cccttgacga tctcgtatgt cccctccgac atactcccgg ccggctgggg
9600 tacgttcgat agcgctatcg gcatcgacaa ggtttgggtc cctagccgat
accgcactac 9660 ctgagtcaca atcttcggag gtttagtctt ccacatagca
cgggcaaaag tgcgtatata 9720 tacaagagcg tttgccagcc acagattttc
actccacaca ccacatcaca catacaacca 9780 cacacatcca caatggaacc
cgaaactaag aagaccaaga ctgactccaa gaagattgtt 9840 cttctcggcg
gcgacttctg tggccccgag gtgattgccg aggccgtcaa ggtgctcaag 9900
tctgttgctg aggcctccgg caccgagttt gtgtttgagg accgactcat tggaggagct
9960 gccattgaga aggagggcga gcccatcacc gacgctactc tcgacatctg
ccgaaaggct 10020 gactctatta tgctcggtgc tgtcggaggc gctgccaaca
ccgtatggac cactcccgac 10080 ggacgaaccg acgtgcgacc cgagcagggt
ctcctcaagc tgcgaaagga cctgaacctg 10140 tacgccaacc tgcgaccctg
ccagctgctg tcgcccaagc tcgccgatct ctcccccatc 10200 cgaaacgttg
agggcaccga cttcatcatt gtccgagagc tcgtcggagg tatctacttt 10260
ggagagcgaa aggaggatga cggatctggc gtcgcttccg acaccgagac ctactccgtt
10320 cctgaggttg agcgaattgc ccgaatggcc gccttcctgg cccttcagca
caacccccct 10380 cttcccgtgt ggtctcttga caaggccaac gtgctggcct
cctctcgact ttggcgaaag 10440 actgtcactc gagtcctcaa ggacgaactc
ccccagctcg agctcaacca ccagctgatc 10500 gactcggccg ccatgatcct
catcaagcag ccctccaaga tgaatggtat catcatcacc 10560 accaacatgt
ttggcgatat catctccgac gaggcctccg tcatccccgg ttctctgggt 10620
ctgctgccct ccgcctctct ggcttctctg cccgacacca acgaggcgtt cggtctgtac
10680 gagccctgtc acggatctgc ccccgatctc ggcaagcaga aggtcaaccc
cattgccacc 10740 attctgtctg ccgccatgat gctcaagttc tctcttaaca
tgaagcccgc cggtgacgct 10800 gttgaggctg ccgtcaagga gtccgtcgag
gctggtatca ctaccgccga tatcggaggc 10860 tcttcctcca cctccgaggt
cggagacttg ttgccaacaa ggtcaaggag ctgctcaaga 10920 aggagtaagt
cgtttctacg acgcattgat ggaaggagca aactgacgcg cctgcgggtt 10980
ggtctaccgg cagggtccgc tagtgtataa gactctataa aaagggccct gccctgctaa
11040 tgaaatgatg atttataatt taccggtgta gcaaccttga ctagaagaag
cagattgggt 11100 gtgtttgtag tggaggacag tggtacgttt tggaaacagt
cttcttgaaa gtgtcttgtc 11160 tacagtatat tcactcataa cctcaatagc
caagggtgta gtcggtttat taaaggaagg 11220 gagttgtggc tgatgtggat
atcgatagtt ggagcaaggg agaaatgtag agtgtgaaag 11280 actcactatg
gtccgggctt atctcgacca atagccaaag tctggagttt ctgagagaaa 11340
aaggcaagat acgtatgtaa caaagcgacg catggtacaa taataccgga ggcatgtatc
11400 atagagagtt agtggttcga tgatggcact ggtgcctggt atgactttat
acggctgact 11460 acatatttgt cctcagacat acaattacag tcaagcactt
acccttggac atctgtaggt 11520 accccccggc caagacgatc tcagcgtgtc
gtatgtcgga ttggcgtagc tccctcgctc 11580 gtcaattggc tcccatctac
tttcttctgc ttggctacac ccagcatgtc tgctatggct 11640 cgttttcgtg
ccttatctat cctcccagta ttaccaactc taaatgacat gatgtgattg 11700
ggtctacact ttcatatcag agataaggag tagcacagtt gcataaaaag cccaactcta
11760 atcagcttct tcctttcttg taattagtac aaaggtgatt agcgaaatct
ggaagcttag 11820 ttggccctaa aaaaatcaaa aaaagcaaaa aacgaaaaac
gaaaaaccac agttttgaga 11880 acagggaggt aacgaaggat cgtatatata
tatatatata tatataccca cggatcccga 11940 gaccggcctt tgattcttcc
ctacaaccaa ccattctcac caccctaatt cacaaccatg 12000 gctcccgacg
ccgacaagct gcgacagcga aaggctcagt ccatccagga cactgccgat 12060
tctcaggcta ccgagctcaa gattggcacc ctgaagggtc tccaaggcac cgagatcgtc
12120 attgatggcg acatctacga catcaaagac ttcgatcacc ctggaggcga
atccatcatg 12180 acctttggtg gcaacgacgt tactgccacc tacaagatga
ttcatcccta ccactcgaag 12240 catcacctgg agaagatgaa aaaggtcggt
cgagtgcccg actacacctc cgagtacaag 12300 ttcgatactc ccttcgaacg
agagatcaaa caggaggtct tcaagattgt gcgaagaggt 12360 cgagagtttg
gaacacctgg ctacttcttt cgagccttct gctacatcgg tctcttcttt 12420
tacctgcagt atctctgggt taccactcct accactttcg cccttgctat cttctacggt
12480 gtgtctcagg ccttcattgg cctgaacgtc cagcacgacg ccaaccacgg
agctgcctcc 12540 aaaaagccct ggatcaacaa tttgctcggc ctgggtgccg
actttatcgg aggctccaag 12600 tggctctgga tgaaccagca ctggacccat
cacacttaca ccaaccatca cgagaaggat 12660 cccgacgccc tgggtgcaga
gcctatgctg ctcttcaacg actatccctt gggtcacccc 12720 aagcgaaccc
tcattcatca cttccaagcc ttctactatc tgtttgtcct tgctggctac 12780
tgggtgtctt cggtgttcaa ccctcagatc ctggacctcc agcaccgagg tgcccaggct
12840 gtcggcatga agatggagaa cgactacatt gccaagtctc gaaagtacgc
tatcttcctg 12900 cgactcctgt acatctacac caacattgtg gctcccatcc
agaaccaagg cttttcgctc 12960 accgtcgttg ctcacattct tactatgggt
gtcgcctcca gcctgaccct cgctactctg 13020 ttcgccctct cccacaactt
cgagaacgca gatcgggatc ccacctacga ggctcgaaag 13080 ggaggcgagc
ctgtctgttg gttcaagtcg caggtggaaa cctcctctac ttacggtggc 13140
ttcatttccg gttgccttac aggcggactc aactttcagg tcgagcatca cctgtttcct
13200 cgaatgtcct ctgcctggta cccctacatc gctcctaccg ttcgagaggt
ctgcaaaaag 13260 cacggcgtca agtacgccta ctatccctgg gtgtggcaga
acctcatctc gaccgtcaag 13320 tacctgcatc agtccggaac tggctcgaac
tggaagaacg gtgccaatcc ctactctggc 13380 aagctgtaag cggccgcatg
tacatacaag attatttata gaaatgaatc gcgatcgaac 13440 aaagagtacg
agtgtacgag taggggatga tgataaaagt ggaagaagtt ccgcatcttt 13500
ggatttatca acgtgtagga cgatacttcc tgtaaaaatg caatgtcttt accataggtt
13560 ctgctgtaga tgttattaac taccattaac atgtctactt gtacagttgc
agaccagttg 13620 gagtatagaa tggtacactt accaaaaagt gttgatggtt
gtaactacga tatataaaac 13680 tgttgacggg atctgcgtac actgttt 13707
<210> SEQ ID NO 75 <211> LENGTH: 1350 <212> TYPE:
DNA <213> ORGANISM: Euglena gracilis <220> FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
synthetic delta-5 desaturase (codon-optimized) for Yarrowia
lipolytica <400> SEQUENCE: 75 atggctctct cccttactac
cgagcagctg ctcgagcgac ccgacctggt tgccatcgac 60 ggcattctct
acgatctgga aggtcttgcc aaggtccatc ccggaggcga cttgatcctc 120
gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc
180 gagaactcga agctgcttca acagttcgtg cgaggcaagc acgaccgaac
ctccaaggac 240 attgtctaca cctacgactc tccctttgca caggacgtca
agcgaactat gcgagaggtc 300 atgaaaggtc ggaactggta tgccacacct
ggattctggc tgcgaaccgt tggcatcatt 360 gctgtcaccg ccttttgcga
gtggcactgg gctactaccg gaatggtgct gtggggtctc 420 ttgactggat
tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt 480
gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga
540 tcgtccagat ggatctggct gcagtctcac atcatgcgac atcacaccta
caccaatcag 600 catggtctcg acctggatgc cgagtccgca gaaccattcc
ttgtgttcca caactaccct 660 gctgccaaca ctgctcgaaa gtggtttcac
cgattccagg cctggtacat gtacctcgtg 720 cttggagcct acggcgtttc
gctggtgtac aaccctctct acatcttccg aatgcagcac 780 aacgacacca
ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac 840
cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg
900 tatctcactg gaacctccct gctcatcacc attcctctgg tgcccactgc
taccggtgcc 960 ttcctcacct tctttttcat cttgtctcac aacttcgatg
gctcggagcg aatccccgac 1020 aagaactgca aggtcaagag ctccgagaag
gacgttgaag ccgatcagat cgactggtac 1080 agagctcagg tggagacctc
ttccacctac ggtggaccca ttgccatgtt ctttactggc 1140 ggtctcaact
tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc 1200
ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac
1260 cctaccattg tgggcaacat catttccacc ctcaagtaca tgcacaaagt
cggtgtggtt 1320 cactgtgtca aggacgctca ggattcctaa 1350 <210>
SEQ ID NO 76 <211> LENGTH: 449 <212> TYPE: PRT
<213> ORGANISM: Euglena gracilis <400> SEQUENCE: 76 Met
Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 1 5 10
15 Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val
20 25 30 His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp
Ala Ser 35 40 45 Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro
Glu Asn Ser Lys 50 55 60 Leu Leu Gln Gln Phe Val Arg Gly Lys His
Asp Arg Thr Ser Lys Asp 65 70 75 80 Ile Val Tyr Thr Tyr Asp Ser Pro
Phe Ala Gln Asp Val Lys Arg Thr 85 90 95 Met Arg Glu Val Met Lys
Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 100 105 110 Trp Leu Arg Thr
Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 115 120 125 His Trp
Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 130 135 140
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 145
150 155 160 Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr
Gly Ile 165 170 175 Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln
Ser His Ile Met 180 185 190 Arg His His Thr Tyr Thr Asn Gln His Gly
Leu Asp Leu Asp Ala Glu 195 200 205 Ser Ala Glu Pro Phe Leu Val Phe
His Asn Tyr Pro Ala Ala Asn Thr 210 215 220 Ala Arg Lys Trp Phe His
Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 225 230 235 240 Leu Gly Ala
Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 245 250 255 Arg
Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 260 265
270 Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg
275 280 285 Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu
Thr Gly 290 295 300 Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr
Ala Thr Gly Ala 305 310 315 320 Phe Leu Thr Phe Phe Phe Ile Leu Ser
His Asn Phe Asp Gly Ser Glu 325 330 335 Arg Ile Pro Asp Lys Asn Cys
Lys Val Lys Ser Ser Glu Lys Asp Val 340 345 350 Glu Ala Asp Gln Ile
Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 355 360 365 Thr Tyr Gly
Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 370 375 380 Gln
Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 385 390
395 400 Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val
Arg 405 410 415 Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser
Thr Leu Lys 420 425 430 Tyr Met His Lys Val Gly Val Val His Cys Val
Lys Asp Ala Gln Asp 435 440 445 Ser <210> SEQ ID NO 77
<211> LENGTH: 1392 <212> TYPE: DNA <213>
ORGANISM: Peridinium sp. CCMP626 <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: synthetic
delta-5 desaturase (codon-optimized) for Yarrowia lipolytica
<400> SEQUENCE: 77 atggctcccg acgccgacaa gctgcgacag
cgaaaggctc agtccatcca ggacactgcc 60 gattctcagg ctaccgagct
caagattggc accctgaagg gtctccaagg caccgagatc 120 gtcattgatg
gcgacatcta cgacatcaaa gacttcgatc accctggagg cgaatccatc 180
atgacctttg gtggcaacga cgttactgcc acctacaaga tgattcatcc ctaccactcg
240 aagcatcacc tggagaagat gaaaaaggtc ggtcgagtgc ccgactacac
ctccgagtac 300 aagttcgata ctcccttcga acgagagatc aaacaggagg
tcttcaagat tgtgcgaaga 360 ggtcgagagt ttggaacacc tggctacttc
tttcgagcct tctgctacat cggtctcttc 420 ttttacctgc agtatctctg
ggttaccact cctaccactt tcgcccttgc tatcttctac 480 ggtgtgtctc
aggccttcat tggcctgaac gtccagcacg acgccaacca cggagctgcc 540
tccaaaaagc cctggatcaa caatttgctc ggcctgggtg ccgactttat cggaggctcc
600 aagtggctct ggatgaacca gcactggacc catcacactt acaccaacca
tcacgagaag 660 gatcccgacg ccctgggtgc agagcctatg ctgctcttca
acgactatcc cttgggtcac 720 cccaagcgaa ccctcattca tcacttccaa
gccttctact atctgtttgt ccttgctggc 780 tactgggtgt cttcggtgtt
caaccctcag atcctggacc tccagcaccg aggtgcccag 840 gctgtcggca
tgaagatgga gaacgactac attgccaagt ctcgaaagta cgctatcttc 900
ctgcgactcc tgtacatcta caccaacatt gtggctccca tccagaacca aggcttttcg
960 ctcaccgtcg ttgctcacat tcttactatg ggtgtcgcct ccagcctgac
cctcgctact 1020 ctgttcgccc tctcccacaa cttcgagaac gcagatcggg
atcccaccta cgaggctcga 1080 aagggaggcg agcctgtctg ttggttcaag
tcgcaggtgg aaacctcctc tacttacggt 1140 ggcttcattt ccggttgcct
tacaggcgga ctcaactttc aggtcgagca tcacctgttt 1200 cctcgaatgt
cctctgcctg gtacccctac atcgctccta ccgttcgaga ggtctgcaaa 1260
aagcacggcg tcaagtacgc ctactatccc tgggtgtggc agaacctcat ctcgaccgtc
1320 aagtacctgc atcagtccgg aactggctcg aactggaaga acggtgccaa
tccctactct 1380 ggcaagctgt aa 1392 <210> SEQ ID NO 78
<211> LENGTH: 463 <212> TYPE: PRT <213> ORGANISM:
Peridinium sp. CCMP626 <400> SEQUENCE: 78 Met Ala Pro Asp Ala
Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 1 5 10 15 Gln Asp Thr
Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 20 25 30 Lys
Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr Asp 35 40
45 Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met Thr Phe Gly
50 55 60 Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile His Pro Tyr
His Ser 65 70 75 80 Lys His His Leu Glu Lys Met Lys Lys Val Gly Arg
Val Pro Asp Tyr 85 90 95 Thr Ser Glu Tyr Lys Phe Asp Thr Pro Phe
Glu Arg Glu Ile Lys Gln 100 105 110 Glu Val Phe Lys Ile Val Arg Arg
Gly Arg Glu Phe Gly Thr Pro Gly 115 120 125 Tyr Phe Phe Arg Ala Phe
Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 130 135 140 Tyr Leu Trp Val
Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 145 150 155 160 Gly
Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala Asn 165 170
175 His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu Leu Gly Leu
180 185 190 Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu Trp Met Asn
Gln His 195 200 205 Trp Thr His His Thr Tyr Thr Asn His His Glu Lys
Asp Pro Asp Ala 210 215 220 Leu Gly Ala Glu Pro Met Leu Leu Phe Asn
Asp Tyr Pro Leu Gly His 225 230 235 240 Pro Lys Arg Thr Leu Ile His
His Phe Gln Ala Phe Tyr Tyr Leu Phe 245 250 255 Val Leu Ala Gly Tyr
Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 260 265 270 Asp Leu Gln
His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 275 280 285 Asp
Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu Leu 290 295
300 Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln Gly Phe Ser
305 310 315 320 Leu Thr Val Val Ala His Ile Leu Thr Met Gly Val Ala
Ser Ser Leu 325 330 335 Thr Leu Ala Thr Leu Phe Ala Leu Ser His Asn
Phe Glu Asn Ala Asp 340 345 350 Arg Asp Pro Thr Tyr Glu Ala Arg Lys
Gly Gly Glu Pro Val Cys Trp 355 360 365 Phe Lys Ser Gln Val Glu Thr
Ser Ser Thr Tyr Gly Gly Phe Ile Ser 370 375 380 Gly Cys Leu Thr Gly
Gly Leu Asn Phe Gln Val Glu His His Leu Phe 385 390 395 400 Pro Arg
Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg 405 410 415
Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro Trp Val 420
425 430 Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His Gln Ser Gly
Thr 435 440 445 Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro Tyr Ser Gly
Lys Leu 450 455 460 <210> SEQ ID NO 79 <211> LENGTH:
1350 <212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (1)..(1350) <223> OTHER INFORMATION: delta-5
desaturase <400> SEQUENCE: 79 atggctctca gtcttaccac
agaacagctg ttagaacgcc ctgatttggt tgcgattgat 60 ggcatcctct
acgaccttga agggcttgcc aaagttcatc caggaggaga tttgattctc 120
gcttctggtg cctctgatgc ctcccctctc ttttattcaa tgcatccata cgtcaaaccg
180 gagaattcca aattgcttca acagttcgtc cgagggaagc atgaccgcac
ctcgaaggac 240 attgtctaca cgtatgattc tcccttcgca caagacgtta
agcggacaat gcgcgaggtg 300 atgaaaggga ggaactggta cgcaacccct
ggcttctggc tgcgcaccgt tgggatcatc 360 gccgtgacgg ccttttgcga
gtggcactgg gctaccacgg ggatggtgct gtggggcctg 420 ttgactggat
tcatgcacat gcagatcggc ttatccatcc agcatgatgc gtcccacggg 480
gccatcagca agaagccttg ggtcaacgcc ctcttcgcct acggcattga cgtcatcgga
540 tcgtcccggt ggatttggct gcagtcgcac atcatgcggc accacaccta
caccaaccag 600 cacggcctcg acctggatgc ggagtcggca gagccgttcc
tggtgttcca caactacccc 660 gccgcaaaca ccgcccgaaa gtggttccac
cgcttccaag cttggtacat gtaccttgtg 720 ctgggggcat acggggtatc
gctggtgtac aacccgctct acattttccg gatgcagcac 780 aatgacacca
tcccagagtc tgtcacggcc atgcgggaga atggctttct gcggcgctac 840
cgcacacttg cattcgtgat gcgagctttc ttcatcttcc ggaccgcatt cttgccctgg
900 tacctcactg ggacctcatt gctgatcacc attcctctgg tgcccactgc
aactggtgcc 960 ttcttgacgt tcttcttcat tttgtcccac aattttgatg
gctccgaacg gatccccgac 1020 aagaactgca aggttaagag ctctgagaag
gacgttgagg ctgaccaaat tgactggtat 1080 cgggcgcagg tggagacgtc
ctccacatac ggtggcccca tcgccatgtt cttcactggc 1140 ggtctcaatt
tccagatcga gcaccacctc tttccccgga tgtcgtcttg gcactacccc 1200
ttcgtccagc aggcggtccg ggagtgttgc gaacgccatg gagtgcgata tgttttctac
1260 cctaccatcg tcggcaacat catctccacc ctgaagtaca tgcataaggt
gggtgtcgtc 1320 cactgcgtga aggacgcaca ggattcctga 1350 <210>
SEQ ID NO 80 <211> LENGTH: 7222 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pFBAIn-MOD-1 <400>
SEQUENCE: 80 catggatcca ggcctgttaa cggccattac ggcctgcagg atccgaaaaa
acctcccaca 60 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
gttgttaact tgtttattgc 120 agcttataat ggttacaaat aaagcaatag
catcacaaat ttcacaaata aagcattttt 180 ttcactgcat tctagttgtg
gtttgtccaa actcatcaat gtatcttatc atgtctgcgg 240 ccgcaagtgt
ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga 300
tggatggatt caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg
360 atatttatgt ttgacacttg agaatgtacg atacaagcac tgtccaagta
caatactaaa 420 catactgtac atactcatac tcgtacccgg gcaacggttt
cacttgagtg cagtggctag 480 tgctcttact cgtacagtgt gcaatactgc
gtatcatagt ctttgatgta tatcgtattc 540 attcatgtta gttgcgtacg
agccggaagc ataaagtgta aagcctgggg tgcctaatga 600 gtgagctaac
tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 660
tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
720 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
gcggcgagcg 780 gtatcagctc actcaaaggc ggtaatacgg ttatccacag
aatcagggga taacgcagga 840 aagaacatgt gagcaaaagg ccagcaaaag
gccaggaacc gtaaaaaggc cgcgttgctg 900 gcgtttttcc ataggctccg
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 960 aggtggcgaa
acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 1020
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1080 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
gtaggtcgtt 1140 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
ccgaccgctg cgccttatcc 1200 ggtaactatc gtcttgagtc caacccggta
agacacgact tatcgccact ggcagcagcc 1260 actggtaaca ggattagcag
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1320 tggcctaact
acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 1380
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
1440 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
tcaagaagat 1500 cctttgatct tttctacggg gtctgacgct cagtggaacg
aaaactcacg ttaagggatt 1560 ttggtcatga gattatcaaa aaggatcttc
acctagatcc ttttaaatta aaaatgaagt 1620 tttaaatcaa tctaaagtat
atatgagtaa acttggtctg acagttacca atgcttaatc 1680 agtgaggcac
ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 1740
gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
1800 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
agccggaagg 1860 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
tccagtctat taattgttgc 1920 cgggaagcta gagtaagtag ttcgccagtt
aatagtttgc gcaacgttgt tgccattgct 1980 acaggcatcg tggtgtcacg
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 2040 cgatcaaggc
gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 2100
cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
2160 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
tggtgagtac 2220 tcaaccaagt cattctgaga atagtgtatg cggcgaccga
gttgctcttg cccggcgtca 2280 atacgggata ataccgcgcc acatagcaga
actttaaaag tgctcatcat tggaaaacgt 2340 tcttcggggc gaaaactctc
aaggatctta ccgctgttga gatccagttc gatgtaaccc 2400 actcgtgcac
ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 2460
aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
2520 ctcatactct tcctttttca atattattga agcatttatc agggttattg
tctcatgagc 2580 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
gggttccgcg cacatttccc 2640 cgaaaagtgc cacctgacgc gccctgtagc
ggcgcattaa gcgcggcggg tgtggtggtt 2700 acgcgcagcg tgaccgctac
acttgccagc gccctagcgc ccgctccttt cgctttcttc 2760 ccttcctttc
tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 2820
ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
2880 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
gttggagtcc 2940 acgttcttta atagtggact cttgttccaa actggaacaa
cactcaaccc tatctcggtc 3000 tattcttttg atttataagg gattttgccg
atttcggcct attggttaaa aaatgagctg 3060 atttaacaaa aatttaacgc
gaattttaac aaaatattaa cgcttacaat ttccattcgc 3120 cattcaggct
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 3180
agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
3240 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
tagggcgaat 3300 tgggtaccgg gccccccctc gaggtcgatg gtgtcgataa
gcttgatatc gaattcatgt 3360 cacacaaacc gatcttcgcc tcaaggaaac
ctaattctac atccgagaga ctgccgagat 3420 ccagtctaca ctgattaatt
ttcgggccaa taatttaaaa aaatcgtgtt atataatatt 3480 atatgtatta
tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga 3540
cagactccat ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg
3600 tttaataata aacagactcc atctaccgcc tccaaatgat gttctcaaaa
tatattgtat 3660 gaacttattt ttattactta gtattattag acaacttact
tgctttatga aaaacacttc 3720 ctatttagga aacaatttat aatggcagtt
cgttcattta acaatttatg tagaataaat 3780 gttataaatg cgtatgggaa
atcttaaata tggatagcat aaatgatatc tgcattgcct 3840 aattcgaaat
caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa 3900
tatcaactat caaagaacag ctattcacac gttactattg agattattat tggacgagaa
3960 tcacacactc aactgtcttt ctctcttcta gaaatacagg tacaagtatg
tactattctc 4020 attgttcata cttctagtca tttcatccca catattcctt
ggatttctct ccaatgaatg 4080 acattctatc ttgcaaattc aacaattata
ataagatata ccaaagtagc ggtatagtgg 4140 caatcaaaaa gcttctctgg
tgtgcttctc gtatttattt ttattctaat gatccattaa 4200 aggtatatat
ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata 4260
aaggtatttt gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt
4320 aatggtagga aattaccata cttttgaaga agcaaaaaaa atgaaagaaa
aaaaaaatcg 4380 tatttccagg ttagacgttc cgcagaatct agaatgcggt
atgcggtaca ttgttcttcg 4440 aacgtaaaag ttgcgctccc tgagatattg
tacatttttg cttttacaag tacaagtaca 4500 tcgtacaact atgtactact
gttgatgcat ccacaacagt ttgttttgtt tttttttgtt 4560 tttttttttt
ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg 4620
ggttattggc gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta
4680 cttttagctt atgcatgcta cttgggtgta atattgggat ctgttcggaa
atcaacggat 4740 gctcaatcga tttcgacagt aattaattaa gtcatacaca
agtcagcttt cttcgagcct 4800 catataagta taagtagttc aacgtattag
cactgtaccc agcatctccg tatcgagaaa 4860 cacaacaaca tgccccattg
gacagatcat gcggatacac aggttgtgca gtatcataca 4920 tactcgatca
gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca 4980
cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
5040 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag
gatctcggtt 5100 ctggccgtac agacctcggc cgacaattat gatatccgtt
ccggtagaca tgacatcctc 5160 aacagttcgg tactgctgtc cgagagcgtc
tcccttgtcg tcaagaccca ccccgggggt 5220 cagaataagc cagtcctcag
agtcgccctt aggtcggttc tgggcaatga agccaaccac 5280 aaactcgggg
tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag 5340
agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
5400 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt
cctccttctt 5460 ctgttcagag acagtttcct cggcaccagc tcgcaggcca
gcaatgattc cggttccggg 5520 tacaccgtgg gcgttggtga tatcggacca
ctcggcgatt cggtgacacc ggtactggtg 5580 cttgacagtg ttgccaatat
ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt 5640 aagagcaagt
tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc 5700
gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc
5760 cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg
ccacgagctt 5820 gagcactcga gcggcaaagg cggacttgtg gacgttagct
cgagcttcgt aggagggcat 5880 tttggtggtg aagaggagac tgaaataaat
ttagtctgca gaacttttta tcggaacctt 5940 atctggggca gtgaagtata
tgttatggta atagttacga gttagttgaa cttatagata 6000 gactggacta
tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc 6060
gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt
6120 gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca
acgaagaatg 6180 tatcgtcaaa gtgatccaag cacactcata gttggagtcg
tactccaaag gcggcaatga 6240 cgagtcagac agatactcgt cgaaaacagt
gtacgcagat ctactataga ggaacattta 6300 aattgccccg gagaagacgg
ccaggccgcc tagatgacaa attcaacaac tcacagctga 6360 ctttctgcca
ttgccactag gggggggcct ttttatatgg ccaagccaag ctctccacgt 6420
cggttgggct gcacccaaca ataaatgggt agggttgcac caacaaaggg atgggatggg
6480 gggtagaaga tacgaggata acggggctca atggcacaaa taagaacgaa
tactgccatt 6540 aagactcgtg atccagcgac tgacaccatt gcatcatcta
agggcctcaa aactacctcg 6600 gaactgctgc gctgatctgg acaccacaga
ggttccgagc actttaggtt gcaccaaatg 6660 tcccaccagg tgcaggcaga
aaacgctgga acagcgtgta cagtttgtct taacaaaaag 6720 tgagggcgct
gaggtcgagc agggtggtgt gacttgttat agcctttaga gctgcgaaag 6780
cgcgtatgga tttggctcat caggccagat tgagggtctg tggacacatg tcatgttagt
6840 gtacttcaat cgccccctgg atatagcccc gacaataggc cgtggcctca
tttttttgcc 6900 ttccgcacat ttccattgct cggtacccac accttgcttc
tcctgcactt gccaacctta 6960 atactggttt acattgacca acatcttaca
agcggggggc ttgtctaggg tatatataaa 7020 cagtggctct cccaatcggt
tgccagtctc ttttttcctt tctttcccca cagattcgaa 7080 atctaaacta
cacatcacag aattccgagc cgtgagtatc cacgacaaga tcagtgtcga 7140
gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct agcaacacac actctctaca
7200 caaactaacc cagctctggt ac 7222 <210> SEQ ID NO 81
<211> LENGTH: 1086 <212> TYPE: DNA <213>
ORGANISM: Phytophthora sojae <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: synthetic
delta-17 desaturase (codon-optimized) <400> SEQUENCE: 81
atggctacca agcagcccta ccagttccct actctgaccg agatcaagcg atctctgccc
60 tccgagtgtt tcgaggcctc cgtgcctctc tctctgtact acaccgttcg
atgcctggtc 120 attgctgtgt cgctcgcctt cggacttcac catgcacgat
ctctgcccgt tgtcgaaggc 180 ctctgggctc tggatgccgc tctctgcacc
ggttacgtgc tgctccaggg catcgtcttc 240 tggggattct ttactgttgg
tcacgacgct ggacatggtg ccttctcccg ataccacctg 300 ctcaactttg
tcatcggaac cttcattcac tctctcatcc ttacaccctt cgagtcctgg 360
aagctcaccc acagacacca tcacaagaac actggcaaca tcgaccgaga cgaaatcttc
420 taccctcaac gaaaggccga cgatcatcct ctgtctcgaa acctcattct
ggctttgggt 480 gcagcctggt ttgcctacct ggtcgaaggc tttcctcccc
gaaaggtcaa ccacttcaac 540 cccttcgagc ctctctttgt tcgacaggtc
tctgccgtgg tcatttcgct ggctgcgcac 600 tttggagtgg ctgccctgtc
catctacctc agcctgcagt tcggcttcaa gactatggcc 660 atctactact
atggtcccgt ctttgtgttc ggatccatgc tcgtcattac tacctttctt 720
catcacaacg acgaagagac accttggtac gcagattcgg agtggaccta cgtcaaaggc
780 aacctgtcct ctgtcgaccg atcctacggt gccctcatcg acaacctttc
tcacaacatc 840 ggaacccacc agattcatca cctctttccc atcattcctc
actacaagct caagcgagct 900 accgaggcct tccatcaagc ctttcccgag
ctggttcgaa agtccgacga acccatcatc 960 aaggcctttt tcagagtcgg
ccgactctac gcaaactacg gtgtggtcga ctcggatgcc 1020 aagctgttca
ctctcaagga ggccaaggct gtttccgaag ccgctaccaa gactaaggcc 1080 acctaa
1086 <210> SEQ ID NO 82 <211> LENGTH: 361 <212>
TYPE: PRT <213> ORGANISM: Phytophthora sojae <220>
FEATURE: <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: synthetic delta-17 desaturase (codon-optimized)
<400> SEQUENCE: 82 Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro
Thr Leu Thr Glu Ile Lys 1 5 10 15 Arg Ser Leu Pro Ser Glu Cys Phe
Glu Ala Ser Val Pro Leu Ser Leu 20 25 30 Tyr Tyr Thr Val Arg Cys
Leu Val Ile Ala Val Ser Leu Ala Phe Gly 35 40 45 Leu His His Ala
Arg Ser Leu Pro Val Val Glu Gly Leu Trp Ala Leu 50 55 60 Asp Ala
Ala Leu Cys Thr Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 65 70 75 80
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 85
90 95 Arg Tyr His Leu Leu Asn Phe Val Ile Gly Thr Phe Ile His Ser
Leu 100 105 110 Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg
His His His 115 120 125 Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile
Phe Tyr Pro Gln Arg 130 135 140 Lys Ala Asp Asp His Pro Leu Ser Arg
Asn Leu Ile Leu Ala Leu Gly 145 150 155 160 Ala Ala Trp Phe Ala Tyr
Leu Val Glu Gly Phe Pro Pro Arg Lys Val 165 170 175 Asn His Phe Asn
Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ser Ala 180 185 190 Val Val
Ile Ser Leu Ala Ala His Phe Gly Val Ala Ala Leu Ser Ile 195 200 205
Tyr Leu Ser Leu Gln Phe Gly Phe Lys Thr Met Ala Ile Tyr Tyr Tyr 210
215 220 Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe
Leu 225 230 235 240 His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp
Ser Glu Trp Thr 245 250 255 Tyr Val Lys Gly Asn Leu Ser Ser Val Asp
Arg Ser Tyr Gly Ala Leu 260 265 270 Ile Asp Asn Leu Ser His Asn Ile
Gly Thr His Gln Ile His His Leu 275 280 285 Phe Pro Ile Ile Pro His
Tyr Lys Leu Lys Arg Ala Thr Glu Ala Phe 290 295 300 His Gln Ala Phe
Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Ile 305 310 315 320 Lys
Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 325 330
335 Asp Ser Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Val Ser
340 345 350 Glu Ala Ala Thr Lys Thr Lys Ala Thr 355 360 <210>
SEQ ID NO 83 <211> LENGTH: 3806 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pPsD17S <400>
SEQUENCE: 83 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt
aatcatggtc 60 atagctgttt cctgtgtgaa attgttatcc gctcacaatt
ccacacaaca tacgagccgg 120 aagcataaag tgtaaagcct ggggtgccta
atgagtgagc taactcacat taattgcgtt 180 gcgctcactg cccgctttcc
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 240 ccaacgcgcg
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 300
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
360 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
aaggccagca 420 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
ttccataggc tccgcccccc 480 tgacgagcat cacaaaaatc gacgctcaag
tcagaggtgg cgaaacccga caggactata 540 aagataccag gcgtttcccc
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 600 gcttaccgga
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 660
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
720 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
agtccaaccc 780 ggtaagacac gacttatcgc cactggcagc agccactggt
aacaggatta gcagagcgag 840 gtatgtaggc ggtgctacag agttcttgaa
gtggtggcct aactacggct acactagaag 900 aacagtattt ggtatctgcg
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 960 ctcttgatcc
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 1020
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
1080 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
caaaaaggat 1140 cttcacctag atccttttaa attaaaaatg aagttttaaa
tcaatctaaa gtatatatga 1200 gtaaacttgg tctgacagtt accaatgctt
aatcagtgag gcacctatct cagcgatctg 1260 tctatttcgt tcatccatag
ttgcctgact ccccgtcgtg tagataacta cgatacggga 1320 gggcttacca
tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 1380
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
1440 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
gtagttcgcc 1500 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
atcgtggtgt cacgctcgtc 1560 gtttggtatg gcttcattca gctccggttc
ccaacgatca aggcgagtta catgatcccc 1620 catgttgtgc aaaaaagcgg
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 1680 ggccgcagtg
ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 1740
atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
1800 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
cgccacatag 1860 cagaacttta aaagtgctca tcattggaaa acgttcttcg
gggcgaaaac tctcaaggat 1920 cttaccgctg ttgagatcca gttcgatgta
acccactcgt gcacccaact gatcttcagc 1980 atcttttact ttcaccagcg
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 2040 aaagggaata
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 2100
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
2160 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
acgtctaaga 2220 aaccattatt atcatgacat taacctataa aaataggcgt
atcacgaggc cctttcgtct 2280 cgcgcgtttc ggtgatgacg gtgaaaacct
ctgacacatg cagctcccgg agacggtcac 2340 agcttgtctg taagcggatg
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 2400 tggcgggtgt
cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 2460
ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
2520 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
cttcgctatt 2580 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
agttgggtaa cgccagggtt 2640 ttcccagtca cgacgttgta aaacgacggc
cagtgaattc gagctcggta cctcgcgaat 2700 gcatctagat ccatggctac
caagcagccc taccagttcc ctactctgac cgagatcaag 2760 cgatctctgc
cctccgagtg tttcgaggcc tccgtgcctc tctctctgta ctacaccgtt 2820
cgatgcctgg tcattgctgt gtcgctcgcc ttcggacttc accatgcacg atctctgccc
2880 gttgtcgaag gcctctgggc tctggatgcc gctctctgca ccggttacgt
gctgctccag 2940 ggcatcgtct tctggggatt ctttactgtt ggtcacgacg
ctggacatgg tgccttctcc 3000 cgataccacc tgctcaactt tgtcatcgga
accttcattc actctctcat ccttacaccc 3060 ttcgagtcct ggaagctcac
ccacagacac catcacaaga acactggcaa catcgaccga 3120 gacgaaatct
tctaccctca acgaaaggcc gacgatcatc ctctgtctcg aaacctcatt 3180
ctggctttgg gtgcagcctg gtttgcctac ctggtcgaag gctttcctcc ccgaaaggtc
3240 aaccacttca accccttcga gcctctcttt gttcgacagg tctctgccgt
ggtcatttcg 3300 ctggctgcgc actttggagt ggctgccctg tccatctacc
tcagcctgca gttcggcttc 3360 aagactatgg ccatctacta ctatggtccc
gtctttgtgt tcggatccat gctcgtcatt 3420 actacctttc ttcatcacaa
cgacgaagag acaccttggt acgcagattc ggagtggacc 3480 tacgtcaaag
gcaacctgtc ctctgtcgac cgatcctacg gtgccctcat cgacaacctt 3540
tctcacaaca tcggaaccca ccagattcat cacctctttc ccatcattcc tcactacaag
3600 ctcaagcgag ctaccgaggc cttccatcaa gcctttcccg agctggttcg
aaagtccgac 3660 gaacccatca tcaaggcctt tttcagagtc ggccgactct
acgcaaacta cggtgtggtc 3720 gactcggatg ccaagctgtt cactctcaag
gaggccaagg ctgtttccga agccgctacc 3780 aagactaagg ccacctaagc ggccgc
3806 <210> SEQ ID NO 84 <211> LENGTH: 1086 <212>
TYPE: DNA <213> ORGANISM: Phytophthora ramorum <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: synthetic delta-17 desaturase (codon-optimized)
<400> SEQUENCE: 84 atggctacca agcagcccta ccagttccct
actctgaccg agatcaagcg atctcttccc 60 tccgagtgct ttgaagcctc
ggtccctctg tccttgtact acaccgtgcg aatcgtcgct 120 attgccgttg
ctctggcctt cggactcaac tacgctcgag cccttcccgt ggtcgagtct 180
ctgtgggcac tcgacgctgc cctttgttgc ggttacgttc tgctccaagg cattgtcttc
240 tggggattct ttaccgtggg tcacgatgct ggacatggtg ccttctctcg
ataccacctg 300 ctcaactttg tcgttggcac ctttatccac tccctcattc
ttactccctt cgagtcgtgg 360 aagctcacac atcgacacca tcacaagaac
accggaaaca tcgaccgaga cgaaatcttc 420 taccctcagc gaaaggccga
cgatcatcct ctgtctcgaa acctcgtcct ggctctcggt 480 gccgcttggt
ttgcctacct tgtcgagggc tttcctcccc gaaaggtcaa ccacttcaac 540
cccttcgaac ctctgtttgt gcgacaggtg gctgccgttg tcatttccct ctctgctcac
600 ttcgccgtcc tggcactgtc cgtgtatctg agctttcagt tcggtctcaa
gacaatggct 660 ctgtactact atggacccgt cttcgtgttc ggctccatgc
tcgtcattac tacctttctg 720 catcacaatg acgaggaaac tccttggtac
ggagattccg actggaccta cgtcaagggc 780 aacttgtctt ccgtggaccg
atcttacggt gccttcatcg acaacctctc gcacaacatt 840 ggcacacacc
agatccacca tctgtttccc atcattcctc actacaagct caaccgagcc 900
accgctgcct tccaccaggc ctttcccgaa cttgtccgaa agagcgacga gcccattctc
960 aaggctttct ggagagttgg tcgactttac gccaactacg gagtcgtgga
tcccgacgca 1020 aagctgttta ctctcaagga ggccaaagct gcctccgagg
ctgccaccaa gaccaaggct 1080 acttaa 1086 <210> SEQ ID NO 85
<211> LENGTH: 3806 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pPrD17S <400> SEQUENCE: 85
ggccgcatcg gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc
60 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
acaacatacg 120 agccggaagc ataaagtgta aagcctgggg tgcctaatga
gtgagctaac tcacattaat 180 tgcgttgcgc tcactgcccg ctttccagtc
gggaaacctg tcgtgccagc tgcattaatg 240 aatcggccaa cgcgcgggga
gaggcggttt gcgtattggg cgctcttccg cttcctcgct 300 cactgactcg
ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 360
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
420 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
ataggctccg 480 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
aggtggcgaa acccgacagg 540 actataaaga taccaggcgt ttccccctgg
aagctccctc gtgcgctctc ctgttccgac 600 cctgccgctt accggatacc
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 660 tagctcacgc
tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 720
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
780 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
ggattagcag 840 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
tggcctaact acggctacac 900 tagaagaaca gtatttggta tctgcgctct
gctgaagcca gttaccttcg gaaaaagagt 960 tggtagctct tgatccggca
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1020 gcagcagatt
acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1080
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
1140 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
tctaaagtat 1200 atatgagtaa acttggtctg acagttacca atgcttaatc
agtgaggcac ctatctcagc 1260 gatctgtcta tttcgttcat ccatagttgc
ctgactcccc gtcgtgtaga taactacgat 1320 acgggagggc ttaccatctg
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1380 ggctccagat
ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1440
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
1500 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
tggtgtcacg 1560 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
cgatcaaggc gagttacatg 1620 atcccccatg ttgtgcaaaa aagcggttag
ctccttcggt cctccgatcg ttgtcagaag 1680 taagttggcc gcagtgttat
cactcatggt tatggcagca ctgcataatt ctcttactgt 1740 catgccatcc
gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 1800
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
1860 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
gaaaactctc 1920 aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc 1980 ttcagcatct tttactttca ccagcgtttc
tgggtgagca aaaacaggaa ggcaaaatgc 2040 cgcaaaaaag ggaataaggg
cgacacggaa atgttgaata ctcatactct tcctttttca 2100 atattattga
agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2160
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
2220 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
cgaggccctt 2280 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
cacatgcagc tcccggagac 2340 ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc 2400 gggtgttggc gggtgtcggg
gctggcttaa ctatgcggca tcagagcaga ttgtactgag 2460 agtgcaccat
atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 2520
gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
2580 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
gggtaacgcc 2640 agggttttcc cagtcacgac gttgtaaaac gacggccagt
gaattcgagc tcggtacctc 2700 gcgaatgcat ctagatccat ggctaccaag
cagccctacc agttccctac tctgaccgag 2760 atcaagcgat ctcttccctc
cgagtgcttt gaagcctcgg tccctctgtc cttgtactac 2820 accgtgcgaa
tcgtcgctat tgccgttgct ctggccttcg gactcaacta cgctcgagcc 2880
cttcccgtgg tcgagtctct gtgggcactc gacgctgccc tttgttgcgg ttacgttctg
2940 ctccaaggca ttgtcttctg gggattcttt accgtgggtc acgatgctgg
acatggtgcc 3000 ttctctcgat accacctgct caactttgtc gttggcacct
ttatccactc cctcattctt 3060 actcccttcg agtcgtggaa gctcacacat
cgacaccatc acaagaacac cggaaacatc 3120 gaccgagacg aaatcttcta
ccctcagcga aaggccgacg atcatcctct gtctcgaaac 3180 ctcgtcctgg
ctctcggtgc cgcttggttt gcctaccttg tcgagggctt tcctccccga 3240
aaggtcaacc acttcaaccc cttcgaacct ctgtttgtgc gacaggtggc tgccgttgtc
3300 atttccctct ctgctcactt cgccgtcctg gcactgtccg tgtatctgag
ctttcagttc 3360 ggtctcaaga caatggctct gtactactat ggacccgtct
tcgtgttcgg ctccatgctc 3420 gtcattacta cctttctgca tcacaatgac
gaggaaactc cttggtacgg agattccgac 3480 tggacctacg tcaagggcaa
cttgtcttcc gtggaccgat cttacggtgc cttcatcgac 3540 aacctctcgc
acaacattgg cacacaccag atccaccatc tgtttcccat cattcctcac 3600
tacaagctca accgagccac cgctgccttc caccaggcct ttcccgaact tgtccgaaag
3660 agcgacgagc ccattctcaa ggctttctgg agagttggtc gactttacgc
caactacgga 3720 gtcgtggatc ccgacgcaaa gctgtttact ctcaaggagg
ccaaagctgc ctccgaggct 3780 gccaccaaga ccaaggctac ttaagc 3806
<210> SEQ ID NO 86 <211> LENGTH: 1209 <212> TYPE:
DNA <213> ORGANISM: Fusarium monoliforme <300>
PUBLICATION INFORMATION: <302> TITLE: DELTA-15 DESATURASES
SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY ACIDS IN
OLEAGINOUS PLANTS AND YEAST <310> PATENT DOCUMENT NUMBER: WO
2005/047480 <311> PATENT FILING DATE: 2004-11-10 <312>
PUBLICATION DATE: 2005-05-26 <313> RELEVANT RESIDUES IN SEQ
ID NO: (1)..(1209) <400> SEQUENCE: 86 atggcgactc gacagcgaac
tgccaccact gttgtggtcg aggaccttcc caaggtcact 60 cttgaggcca
agtctgaacc tgtgttcccc gatatcaaga ccatcaagga tgccattccc 120
gcgcactgct tccagccctc gctcgtcacc tcattctact acgtcttccg cgattttgct
180 atggtctctg ccctcgtctg ggctgctctc acctacatcc ccagcatccc
cgaccagacc 240 ctccgcgtcg cagcttggat ggtctacggc ttcgtccagg
gtctgttctg caccggtgtc 300 tggattctcg gccatgagtg cggccacggt
gctttctctc tccacggaaa ggtcaacaat 360 gtgaccggct ggttcctcca
ctcgttcctc ctcgtcccct acttcagctg gaagtactct 420 caccaccgcc
accaccgctt caccggccac atggatctcg acatggcttt cgtccccaag 480
actgagccca agccctccaa gtcgctcatg attgctggca ttgacgtcgc cgagcttgtt
540 gaggacaccc ccgctgctca gatggtcaag ctcatcttcc accagctttt
cggatggcag 600 gcgtacctct tcttcaacgc tagctctggc aagggcagca
agcagtggga gcccaagact 660 ggcctctcca agtggttccg agtcagtcac
ttcgagccta ccagcgctgt cttccgcccc 720 aacgaggcca tcttcatcct
catctccgat atcggtcttg ctctaatggg aactgctctg 780 tactttgctt
ccaagcaagt tggtgtttcg accattctct tcctctacct tgttccctac 840
ctgtgggttc accactggct cgttgccatt acctacctcc accaccacca caccgagctc
900 cctcactaca ccgctgaggg ctggacctac gtcaagggag ctctcgccac
tgtcgaccgt 960 gagtttggct tcatcggaaa gcacctcttc cacggtatca
ttgagaagca cgttgttcac 1020 catctcttcc ctaagatccc cttctacaag
gctgacgagg ccaccgaggc catcaagccc 1080 gtcattggcg accactactg
ccacgacgac cgaagcttcc tgggccagct gtggaccatc 1140 ttcggcacgc
tcaagtacgt cgagcacgac cctgcccgac ccggtgccat gcgatggaac 1200
aaggactag 1209 <210> SEQ ID NO 87 <211> LENGTH: 402
<212> TYPE: PRT <213> ORGANISM: Fusarium monoliforme
<300> PUBLICATION INFORMATION: <302> TITLE: DELTA-15
DESATURASES SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY
ACIDS IN OLEAGINOUS PLANTS AND YEAST <310> PATENT DOCUMENT
NUMBER: WO 2005/047480 <311> PATENT FILING DATE: 2004-11-10
<312> PUBLICATION DATE: 2005-05-26 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(402) <400> SEQUENCE: 87 Met Ala
Thr Arg Gln Arg Thr Ala Thr Thr Val Val Val Glu Asp Leu 1 5 10 15
Pro Lys Val Thr Leu Glu Ala Lys Ser Glu Pro Val Phe Pro Asp Ile 20
25 30 Lys Thr Ile Lys Asp Ala Ile Pro Ala His Cys Phe Gln Pro Ser
Leu 35 40 45 Val Thr Ser Phe Tyr Tyr Val Phe Arg Asp Phe Ala Met
Val Ser Ala 50 55 60 Leu Val Trp Ala Ala Leu Thr Tyr Ile Pro Ser
Ile Pro Asp Gln Thr 65 70 75 80 Leu Arg Val Ala Ala Trp Met Val Tyr
Gly Phe Val Gln Gly Leu Phe 85 90 95 Cys Thr Gly Val Trp Ile Leu
Gly His Glu Cys Gly His Gly Ala Phe 100 105 110 Ser Leu His Gly Lys
Val Asn Asn Val Thr Gly Trp Phe Leu His Ser 115 120 125 Phe Leu Leu
Val Pro Tyr Phe Ser Trp Lys Tyr Ser His His Arg His 130 135 140 His
Arg Phe Thr Gly His Met Asp Leu Asp Met Ala Phe Val Pro Lys 145 150
155 160 Thr Glu Pro Lys Pro Ser Lys Ser Leu Met Ile Ala Gly Ile Asp
Val 165 170 175 Ala Glu Leu Val Glu Asp Thr Pro Ala Ala Gln Met Val
Lys Leu Ile 180 185 190 Phe His Gln Leu Phe Gly Trp Gln Ala Tyr Leu
Phe Phe Asn Ala Ser 195 200 205 Ser Gly Lys Gly Ser Lys Gln Trp Glu
Pro Lys Thr Gly Leu Ser Lys 210 215 220 Trp Phe Arg Val Ser His Phe
Glu Pro Thr Ser Ala Val Phe Arg Pro 225 230 235 240 Asn Glu Ala Ile
Phe Ile Leu Ile Ser Asp Ile Gly Leu Ala Leu Met 245 250 255 Gly Thr
Ala Leu Tyr Phe Ala Ser Lys Gln Val Gly Val Ser Thr Ile 260 265 270
Leu Phe Leu Tyr Leu Val Pro Tyr Leu Trp Val His His Trp Leu Val 275
280 285 Ala Ile Thr Tyr Leu His His His His Thr Glu Leu Pro His Tyr
Thr 290 295 300 Ala Glu Gly Trp Thr Tyr Val Lys Gly Ala Leu Ala Thr
Val Asp Arg 305 310 315 320 Glu Phe Gly Phe Ile Gly Lys His Leu Phe
His Gly Ile Ile Glu Lys 325 330 335 His Val Val His His Leu Phe Pro
Lys Ile Pro Phe Tyr Lys Ala Asp 340 345 350 Glu Ala Thr Glu Ala Ile
Lys Pro Val Ile Gly Asp His Tyr Cys His 355 360 365 Asp Asp Arg Ser
Phe Leu Gly Gln Leu Trp Thr Ile Phe Gly Thr Leu 370 375 380 Lys Tyr
Val Glu His Asp Pro Ala Arg Pro Gly Ala Met Arg Trp Asn 385 390 395
400 Lys Asp <210> SEQ ID NO 88 <211> LENGTH: 7668
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid
pY6.GPD.Leu2 <400> SEQUENCE: 88 gtacgagccg gaagcataaa
gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 60 ttaattgcgt
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 120
taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
180 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
agctcactca 240 aaggcggtaa tacggttatc cacagaatca ggggataacg
caggaaagaa catgtgagca 300 aaaggccagc aaaaggccag gaaccgtaaa
aaggccgcgt tgctggcgtt tttccatagg 360 ctccgccccc ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 420 acaggactat
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 480
ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
540 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
caagctgggc 600 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
tatccggtaa ctatcgtctt 660 gagtccaacc cggtaagaca cgacttatcg
ccactggcag cagccactgg taacaggatt 720 agcagagcga ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc 780 tacactagaa
ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 840
agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
900 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
gatcttttct 960 acggggtctg acgctcagtg gaacgaaaac tcacgttaag
ggattttggt catgagatta 1020 tcaaaaagga tcttcaccta gatcctttta
aattaaaaat gaagttttaa atcaatctaa 1080 agtatatatg agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc 1140 tcagcgatct
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 1200
acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
1260 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
gcgcagaagt 1320 ggtcctgcaa ctttatccgc ctccatccag tctattaatt
gttgccggga agctagagta 1380 agtagttcgc cagttaatag tttgcgcaac
gttgttgcca ttgctacagg catcgtggtg 1440 tcacgctcgt cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1500 acatgatccc
ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1560
agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
1620 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
caagtcattc 1680 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc 1740 gcgccacata gcagaacttt aaaagtgctc
atcattggaa aacgttcttc ggggcgaaaa 1800 ctctcaagga tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac 1860 tgatcttcag
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 1920
aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
1980 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
catatttgaa 2040 tgtatttaga aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct 2100 gacgcgccct gtagcggcgc attaagcgcg
gcgggtgtgg tggttacgcg cagcgtgacc 2160 gctacacttg ccagcgccct
agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 2220 acgttcgccg
gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 2280
agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
2340 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
ctttaatagt 2400 ggactcttgt tccaaactgg aacaacactc aaccctatct
cggtctattc ttttgattta 2460 taagggattt tgccgatttc ggcctattgg
ttaaaaaatg agctgattta acaaaaattt 2520 aacgcgaatt ttaacaaaat
attaacgctt acaatttcca ttcgccattc aggctgcgca 2580 actgttggga
agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 2640
gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
2700 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
accgggcccc 2760 ccctcgaggt cgatggtgtc gataagcttg atatcgaatt
catgtcacac aaaccgatct 2820 tcgcctcaag gaaacctaat tctacatccg
agagactgcc gagatccagt ctacactgat 2880 taattttcgg gccaataatt
taaaaaaatc gtgttatata atattatatg tattatatat 2940 atacatcatg
atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc 3000
gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag
3060 actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact
tatttttatt 3120 acttagtatt attagacaac ttacttgctt tatgaaaaac
acttcctatt taggaaacaa 3180 tttataatgg cagttcgttc atttaacaat
ttatgtagaa taaatgttat aaatgcgtat 3240 gggaaatctt aaatatggat
agcataaatg atatctgcat tgcctaattc gaaatcaaca 3300 gcaacgaaaa
aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag 3360
aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg
3420 tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt
tcatacttct 3480 agtcatttca tcccacatat tccttggatt tctctccaat
gaatgacatt ctatcttgca 3540 aattcaacaa ttataataag atataccaaa
gtagcggtat agtggcaatc aaaaagcttc 3600 tctggtgtgc ttctcgtatt
tatttttatt ctaatgatcc attaaaggta tatatttatt 3660 tcttgttata
taatcctttt gtttattaca tgggctggat acataaaggt attttgattt 3720
aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta
3780 ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt
ccaggttaga 3840 cgttccgcag aatctagaat gcggtatgcg gtacattgtt
cttcgaacgt aaaagttgcg 3900 ctccctgaga tattgtacat ttttgctttt
acaagtacaa gtacatcgta caactatgta 3960 ctactgttga tgcatccaca
acagtttgtt ttgttttttt ttgttttttt tttttctaat 4020 gattcattac
cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca 4080
attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca
4140 tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca
accgatttcg 4200 acagtaatta attaatttga atcgaatcgg agcctaaaat
gaacccgagt atatctcata 4260 aaattctcgg tgagaggtct gtgactgtca
gtacaaggtg ccttcattat gccctcaacc 4320 ttaccatacc tcactgaatg
tagtgtacct ctaaaaatga aatacagtgc caaaagccaa 4380 ggcactgagc
tcgtctaacg gacttgatat acaaccaatt aaaacaaatg aaaagaaata 4440
cagttctttg tatcatttgt aacaattacc ctgtacaaac taaggtattg aaatcccaca
4500 atattcccaa agtccacccc tttccaaatt gtcatgccta caactcatat
accaagcact 4560 aacctaccaa acaccactaa aaccccacaa aatatatctt
accgaatata cagtaacaag 4620 ctaccaccac actcgttggg tgcagtcgcc
agcttaaaga tatctatcca catcagccac 4680 aactcccttc ctttaataaa
ccgactacac ccttggctat tgaggttatg agtgaatata 4740 ctgtagacaa
gacactttca agaagactgt ttccaaaacg taccactgtc ctccactaca 4800
aacacaccca atctgcttct tctagtcaag gttgctacac cggtaaatta taaatcatca
4860 tttcattagc agggcagggc cctttttata gagtcttata cactagcgga
ccctgccggt 4920 agaccaaccc gcaggcgcgt cagtttgctc cttccatcaa
tgcgtcgtag aaacgactta 4980 ctccttcttg agcagctcct tgaccttgtt
ggcaacaagt ctccgacctc ggaggtggag 5040 gaagagcctc cgatatcggc
ggtagtgata ccagcctcga cggactcctt gacggcagcc 5100 tcaacagcgt
caccggcggg cttcatgtta agagagaact tgagcatcat ggcggcagac 5160
agaatggtgg caatggggtt gaccttctgc ttgccgagat cgggggcaga tccgtgacag
5220 ggctcgtaca gaccgaacgc ctcgttggtg tcgggcagag aagccagaga
ggcggagggc 5280 agcagaccca gagaaccggg gatgacggag gcctcgtcgg
agatgatatc gccaaacatg 5340 ttggtggtga tgatgatacc attcatcttg
gagggctgct tgatgaggat catggcggcc 5400 gagtcgatca gctggtggtt
gagctcgagc tgggggaatt cgtccttgag gactcgagtg 5460 acagtctttc
gccaaagtcg agaggaggcc agcacgttgg ccttgtcaag agaccacacg 5520
ggaagagggg ggttgtgctg aagggccagg aaggcggcca ttcgggcaat tcgctcaacc
5580 tcaggaacgg agtaggtctc ggtgtcggaa gcgacgccag atccgtcatc
ctcctttcgc 5640 tctccaaagt agatacctcc gacgagctct cggacaatga
tgaagtcggt gccctcaacg 5700 tttcggatgg gggagagatc ggcgagcttg
ggcgacagca gctggcaggg tcgcaggttg 5760 gcgtacaggt tcaggtcctt
tcgcagcttg aggagaccct gctcgggtcg cacgtcggtt 5820 cgtccgtcgg
gagtggtcca tacggtgttg gcagcgcctc cgacagcacc gagcataata 5880
gagtcagcct ttcggcagat gtcgagagta gcgtcggtga tgggctcgcc ctccttctca
5940 atggcagctc ctccaatgag tcggtcctca aacacaaact cggtgccgga
ggcctcagca 6000 acagacttga gcaccttgac ggcctcggca atcacctcgg
ggccacagaa gtcgccgccg 6060 agaagaacaa tcttcttgga gtcagtcttg
gtcttcttag tttcgggttc cattgtggat 6120 gtgtgtggtt gtatgtgtga
tgtggtgtgt ggagtgaaaa tctgtggctg gcaaacgctc 6180 ttgtatatat
acgcactttt gcccgtgcta tgtggaagac taaacctccg aagattgtga 6240
ctcaggtagt gcggtatcgg ctagggaccc aaaccttgtc gatgccgata gcgctatcga
6300 acgtacccca gccggccggg agtatgtcgg aggggacata cgagatcgtc
aagggtttgt 6360 ggccaactgg taaataaatg atgtcgacgc agtaggatgt
cctgcacggg tctttttgtg 6420 gggtgtggag aaaggggtgc ttggagatgg
aagccggtag aaccgggctg cttgtgcttg 6480 gagatggaag ccggtagaac
cgggctgctt ggggggattt ggggccgctg ggctccaaag 6540 aggggtaggc
atttcgttgg ggttacgtaa ttgcggcatt tgggtcctgc gcgcatgtcc 6600
cattggtcag aattagtccg gataggagac ttatcagcca atcacagcgc cggatccacc
6660 tgtaggttgg gttgggtggg agcacccctc cacagagtag agtcaaacag
cagcagcaac 6720 atgatagttg ggggtgtgcg tgttaaagga aaaaaaagaa
gcttgggtta tattcccgct 6780 ctatttagag gttgcgggat agacgccgac
ggagggcaat ggcgctatgg aaccttgcgg 6840 atatccatac gccgcggcgg
actgcgtccg aaccagctcc agcagcgttt tttccgggcc 6900 attgagccga
ctgcgacccc gccaacgtgt cttggcccac gcactcatgt catgttggtg 6960
ttgggaggcc actttttaag tagcacaagg cacctagctc gcagcaaggt gtccgaacca
7020 aagaagcggc tgcagtggtg caaacggggc ggaaacggcg ggaaaaagcc
acgggggcac 7080 gaattgaggc acgccctcga atttgagacg agtcacggcc
ccattcgccc gcgcaatggc 7140 tcgccaacgc ccggtctttt gcaccacatc
aggttacccc aagccaaacc tttgtgttaa 7200 aaagcttaac atattatacc
gaacgtaggt ttgggcgggc ttgctccgtc tgtccaaggc 7260 aacatttata
taagggtctg catcgccggc tcaattgaat cttttttctt cttctcttct 7320
ctatattcat tcttgaatta aacacacatc aaccatggat ccactagttc tagagcggcc
7380 gccaccgcgg cccgagattc cggcctcttc ggccgccaag cgacccgggt
ggacgtctag 7440 aggtacctag caattaacag atagtttgcc ggtgataatt
ctcttaacct cccacactcc 7500 tttgacataa cgatttatgt aacgaaactg
aaatttgacc agatattgtg tccgcggtgg 7560 agctccagct tttgttccct
ttagtgaggg ttaatttcga gcttggcgta atcatggtca 7620 tagctgtttc
ctgtgtgaaa ttgttatccg ctcacaattc cacacaac 7668 <210> SEQ ID
NO 89 <211> LENGTH: 9048 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pY130 <400> SEQUENCE: 89
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg ctcggtcgtt
cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca 300 aaaggccagc
aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 360
ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
420 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660 gagtccaacc
cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 720
agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
780 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg cagaaaaaaa
ggatctcaag aagatccttt gatcttttct 960 acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta 1020 tcaaaaagga
tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 1080
agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
gtagataact 1200 acgatacggg agggcttacc atctggcccc agtgctgcaa
tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc agcaataaac
cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa ctttatccgc
ctccatccag tctattaatt gttgccggga agctagagta 1380 agtagttcgc
cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1440
tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc atggttatgg
cagcactgca taattctctt 1620 actgtcatgc catccgtaag atgcttttct
gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740 gcgccacata
gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1800
ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
1860 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
gaatactcat actcttcctt 1980 tttcaatatt attgaagcat ttatcagggt
tattgtctca tgagcggata catatttgaa 2040 tgtatttaga aaaataaaca
aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100 gacgcgccct
gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2160
gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa cttgattagg
gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt tccaaactgg
aacaacactc aaccctatct cggtctattc ttttgattta 2460 taagggattt
tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 2520
aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg taatacgact
cactataggg cgaattgggt accgggcccc 2760 ccctcgaggt cgatggtgtc
gataagcttg atatcgaatt catgtcacac aaaccgatct 2820 tcgcctcaag
gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat 2880
taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat
2940 atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac
tccatctgcc 3000 gcctccaact gatgttctca atatttaagg ggtcatctcg
cattgtttaa taataaacag 3060 actccatcta ccgcctccaa atgatgttct
caaaatatat tgtatgaact tatttttatt 3120 acttagtatt attagacaac
ttacttgctt tatgaaaaac acttcctatt taggaaacaa 3180 tttataatgg
cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat 3240
gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca
3300 gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca
actatcaaag 3360 aacagctatt cacacgttac tattgagatt attattggac
gagaatcaca cactcaactg 3420 tctttctctc ttctagaaat acaggtacaa
gtatgtacta ttctcattgt tcatacttct 3480 agtcatttca tcccacatat
tccttggatt tctctccaat gaatgacatt ctatcttgca 3540 aattcaacaa
ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc 3600
tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt
3660 tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt
attttgattt 3720 aattttttgc ttaaattcaa tcccccctcg ttcagtgtca
actgtaatgg taggaaatta 3780 ccatactttt gaagaagcaa aaaaaatgaa
agaaaaaaaa aatcgtattt ccaggttaga 3840 cgttccgcag aatctagaat
gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg 3900 ctccctgaga
tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta 3960
ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat
4020 gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta
ttggcgttca 4080 attaatcata gacttatgaa tctgcacggt gtgcgctgcg
agttactttt agcttatgca 4140 tgctacttgg gtgtaatatt gggatctgtt
cggaaatcaa cggatgctca accgatttcg 4200 acagtaataa tttgaatcga
atcggagcct aaaatgaacc cgagtatatc tcataaaatt 4260 ctcggtgaga
ggtctgtgac tgtcagtaca aggtgccttc attatgccct caaccttacc 4320
atacctcact gaatgtagtg tacctctaaa aatgaaatac agtgccaaaa gccaaggcac
4380 tgagctcgtc taacggactt gatatacaac caattaaaac aaatgaaaag
aaatacagtt 4440 ctttgtatca tttgtaacaa ttaccctgta caaactaagg
tattgaaatc ccacaatatt 4500 cccaaagtcc acccctttcc aaattgtcat
gcctacaact catataccaa gcactaacct 4560 accaaacacc actaaaaccc
cacaaaatat atcttaccga atatacagta acaagctacc 4620 accacactcg
ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc 4680
ccttccttta ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta
4740 gacaagacac tttcaagaag actgtttcca aaacgtacca ctgtcctcca
ctacaaacac 4800 acccaatctg cttcttctag tcaaggttgc tacaccggta
aattataaat catcatttca 4860 ttagcagggc agggcccttt ttatagagtc
ttatacacta gcggaccctg ccggtagacc 4920 aacccgcagg cgcgtcagtt
tgctccttcc atcaatgcgt cgtagaaacg acttactcct 4980 tcttgagcag
ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga 5040
gcctccgata tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac
5100 agcgtcaccg gcgggcttca tgttaagaga gaacttgagc atcatggcgg
cagacagaat 5160 ggtggcaatg gggttgacct tctgcttgcc gagatcgggg
gcagatccgt gacagggctc 5220 gtacagaccg aacgcctcgt tggtgtcggg
cagagaagcc agagaggcgg agggcagcag 5280 acccagagaa ccggggatga
cggaggcctc gtcggagatg atatcgccaa acatgttggt 5340 ggtgatgatg
ataccattca tcttggaggg ctgcttgatg aggatcatgg cggccgagtc 5400
gatcagctgg tggttgagct cgagctgggg gaattcgtcc ttgaggactc gagtgacagt
5460 ctttcgccaa agtcgagagg aggccagcac gttggccttg tcaagagacc
acacgggaag 5520 aggggggttg tgctgaaggg ccaggaaggc ggccattcgg
gcaattcgct caacctcagg 5580 aacggagtag gtctcggtgt cggaagcgac
gccagatccg tcatcctcct ttcgctctcc 5640 aaagtagata cctccgacga
gctctcggac aatgatgaag tcggtgccct caacgtttcg 5700 gatgggggag
agatcggcga gcttgggcga cagcagctgg cagggtcgca ggttggcgta 5760
caggttcagg tcctttcgca gcttgaggag accctgctcg ggtcgcacgt cggttcgtcc
5820 gtcgggagtg gtccatacgg tgttggcagc gcctccgaca gcaccgagca
taatagagtc 5880 agcctttcgg cagatgtcga gagtagcgtc ggtgatgggc
tcgccctcct tctcaatggc 5940 agctcctcca atgagtcggt cctcaaacac
aaactcggtg ccggaggcct cagcaacaga 6000 cttgagcacc ttgacggcct
cggcaatcac ctcggggcca cagaagtcgc cgccgagaag 6060 aacaatcttc
ttggagtcag tcttggtctt cttagtttcg ggttccattg tggatgtgtg 6120
tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt ggctggcaaa cgctcttgta
6180 tatatacgca cttttgcccg tgctatgtgg aagactaaac ctccgaagat
tgtgactcag 6240 gtagtgcggt atcggctagg gacccaaacc ttgtcgatgc
cgatagcgct atcgaacgta 6300 ccccagccgg ccgggagtat gtcggagggg
acatacgaga tcgtcaaggg tttgtggcca 6360 actggtattt aaatgatgtc
gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg 6420 tggagaaagg
ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgt gcttggagat 6480
ggaagccggt agaaccgggc tgcttggggg gatttggggc cgctgggctc caaagagggg
6540 taggcatttc gttggggtta cgtaattgcg gcatttgggt cctgcgcgca
tgtcccattg 6600 gtcagaatta gtccggatag gagacttatc agccaatcac
agcgccggat ccacctgtag 6660 gttgggttgg gtgggagcac ccctccacag
agtagagtca aacagcagca gcaacatgat 6720 agttgggggt gtgcgtgtta
aaggaaaaaa aagaagcttg ggttatattc ccgctctatt 6780 tagaggttgc
gggatagacg ccgacggagg gcaatggcgc tatggaacct tgcggatatc 6840
catacgccgc ggcggactgc gtccgaacca gctccagcag cgttttttcc gggccattga
6900 gccgactgcg accccgccaa cgtgtcttgg cccacgcact catgtcatgt
tggtgttggg 6960 aggccacttt ttaagtagca caaggcacct agctcgcagc
aaggtgtccg aaccaaagaa 7020 gcggctgcag tggtgcaaac ggggcggaaa
cggcgggaaa aagccacggg ggcacgaatt 7080 gaggcacgcc ctcgaatttg
agacgagtca cggccccatt cgcccgcgca atggctcgcc 7140 aacgcccggt
cttttgcacc acatcaggtt accccaagcc aaacctttgt gttaaaaagc 7200
ttaacatatt ataccgaacg taggtttggg cgggcttgct ccgtctgtcc aaggcaacat
7260 ttatataagg gtctgcatcg ccggctcaat tgaatctttt ttcttcttct
cttctctata 7320 ttcattcttg aattaaacac acatcaacca tggcgactcg
acagcgaact gccaccactg 7380 ttgtggtcga ggaccttccc aaggtcactc
ttgaggccaa gtctgaacct gtgttccccg 7440 atatcaagac catcaaggat
gccattcccg cgcactgctt ccagccctcg ctcgtcacct 7500 cattctacta
cgtcttccgc gattttgcta tggtctctgc cctcgtctgg gctgctctca 7560
cctacatccc cagcatcccc gaccagaccc tccgcgtcgc agcttggatg gtctacggct
7620 tcgtccaggg tctgttctgc accggtgtct ggattctcgg ccatgagtgc
ggccacggtg 7680 ctttctctct ccacggaaag gtcaacaatg tgaccggctg
gttcctccac tcgttcctcc 7740 tcgtccccta cttcagctgg aagtactctc
accaccgcca ccaccgcttc accggccaca 7800 tggatctcga catggctttc
gtccccaaga ctgagcccaa gccctccaag tcgctcatga 7860 ttgctggcat
tgacgtcgcc gagcttgttg aggacacccc cgctgctcag atggtcaagc 7920
tcatcttcca ccagcttttc ggatggcagg cgtacctctt cttcaacgct agctctggca
7980 agggcagcaa gcagtgggag cccaagactg gcctctccaa gtggttccga
gtcagtcact 8040 tcgagcctac cagcgctgtc ttccgcccca acgaggccat
cttcatcctc atctccgata 8100 tcggtcttgc tctaatggga actgctctgt
actttgcttc caagcaagtt ggtgtttcga 8160 ccattctctt cctctacctt
gttccctacc tgtgggttca ccactggctc gttgccatta 8220 cctacctcca
ccaccaccac accgagctcc ctcactacac cgctgagggc tggacctacg 8280
tcaagggagc tctcgccact gtcgaccgtg agtttggctt catcggaaag cacctcttcc
8340 acggtatcat tgagaagcac gttgttcacc atctcttccc taagatcccc
ttctacaagg 8400 ctgacgaggc caccgaggcc atcaagcccg tcattggcga
ccactactgc cacgacgacc 8460 gaagcttcct gggccagctg tggaccatct
tcggcacgct caagtacgtc gagcacgacc 8520 ctgcccgacc cggtgccatg
cgatggaaca aggactaggc ggccgcatga gaagataaat 8580 atataaatac
attgagatat taaatgcgct agattagaga gcctcatact gctcggagag 8640
aagccaagac gagtactcaa aggggattac accatccata tccacagaca caagctgggg
8700 aaaggttcta tatacacttt ccggaatacc gtagtttccg atgttatcaa
tgggggcagc 8760 caggatttca ggcacttcgg tgtctcgggg tgaaatggcg
ttcttggcct ccatcaagtc 8820 gtaccatgtc ttcatttgcc tgtcaaagta
aaacagaagc agatgaagaa tgaacttgaa 8880 gtgaaggaat ttaaatgtaa
cgaaactgaa atttgaccag atattgtgtc cgcggtggag 8940 ctccagcttt
tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata 9000
gctgtttcct gtgtgaaatt gttatccgct cacaagcttc cacacaac 9048
<210> SEQ ID NO 90 <211> LENGTH: 8925 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid p138 <400> SEQUENCE:
90 gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
ctaactcaca 60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc
ggtttgcgta ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 300
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
360 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg 420 acaggactat aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
720 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc 780 tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct 960 acggggtctg
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1020
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1080 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc 1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact 1200 acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1380
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1440 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
aaggcgagtt 1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc
atggttatgg cagcactgca taattctctt 1620 actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740
gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
1800 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac 1860 tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt 1980 tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa 2040 tgtatttaga
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2160 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc 2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc
tccctttagg gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa
cttgattagg gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt
tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 2460
taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2520 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
aggctgcgca 2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt
acgccagctg gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa
cgccagggtt ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg
taatacgact cactataggg cgaattgggt accgggcccc 2760 ccctcgaggt
cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct 2820
tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat
2880 taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg
tattatatat 2940 atacatcatg atgatactga cagtcatgtc ccattgctaa
atagacagac tccatctgcc 3000 gcctccaact gatgttctca atatttaagg
ggtcatctcg cattgtttaa taataaacag 3060 actccatcta ccgcctccaa
atgatgttct caaaatatat tgtatgaact tatttttatt 3120 acttagtatt
attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa 3180
tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat
3240 gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc
gaaatcaaca 3300 gcaacgaaaa aaatcccttg tacaacataa atagtcatcg
agaaatatca actatcaaag 3360 aacagctatt cacacgttac tattgagatt
attattggac gagaatcaca cactcaactg 3420 tctttctctc ttctagaaat
acaggtacaa gtatgtacta ttctcattgt tcatacttct 3480 agtcatttca
tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca 3540
aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc
3600 tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta
tatatttatt 3660 tcttgttata taatcctttt gtttattaca tgggctggat
acataaaggt attttgattt 3720 aattttttgc ttaaattcaa tcccccctcg
ttcagtgtca actgtaatgg taggaaatta 3780 ccatactttt gaagaagcaa
aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga 3840 cgttccgcag
aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg 3900
ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta
3960 ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt
tttttctaat 4020 gattcattac cgctatgtat acctacttgt acttgtagta
agccgggtta ttggcgttca 4080 attaatcata gacttatgaa tctgcacggt
gtgcgctgcg agttactttt agcttatgca 4140 tgctacttgg gtgtaatatt
gggatctgtt cggaaatcaa cggatgctca accgatttcg 4200 acagtaataa
tttgaatcga atcggagcct aaaatgaacc cgagtatatc tcataaaatt 4260
ctcggtgaga ggtctgtgac tgtcagtaca aggtgccttc attatgccct caaccttacc
4320 atacctcact gaatgtagtg tacctctaaa aatgaaatac agtgccaaaa
gccaaggcac 4380 tgagctcgtc taacggactt gatatacaac caattaaaac
aaatgaaaag aaatacagtt 4440 ctttgtatca tttgtaacaa ttaccctgta
caaactaagg tattgaaatc ccacaatatt 4500 cccaaagtcc acccctttcc
aaattgtcat gcctacaact catataccaa gcactaacct 4560 accaaacacc
actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc 4620
accacactcg ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc
4680 ccttccttta ataaaccgac tacacccttg gctattgagg ttatgagtga
atatactgta 4740 gacaagacac tttcaagaag actgtttcca aaacgtacca
ctgtcctcca ctacaaacac 4800 acccaatctg cttcttctag tcaaggttgc
tacaccggta aattataaat catcatttca 4860 ttagcagggc agggcccttt
ttatagagtc ttatacacta gcggaccctg ccggtagacc 4920 aacccgcagg
cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct 4980
tcttgagcag ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga
5040 gcctccgata tcggcggtag tgataccagc ctcgacggac tccttgacgg
cagcctcaac 5100 agcgtcaccg gcgggcttca tgttaagaga gaacttgagc
atcatggcgg cagacagaat 5160 ggtggcaatg gggttgacct tctgcttgcc
gagatcgggg gcagatccgt gacagggctc 5220 gtacagaccg aacgcctcgt
tggtgtcggg cagagaagcc agagaggcgg agggcagcag 5280 acccagagaa
ccggggatga cggaggcctc gtcggagatg atatcgccaa acatgttggt 5340
ggtgatgatg ataccattca tcttggaggg ctgcttgatg aggatcatgg cggccgagtc
5400 gatcagctgg tggttgagct cgagctgggg gaattcgtcc ttgaggactc
gagtgacagt 5460 ctttcgccaa agtcgagagg aggccagcac gttggccttg
tcaagagacc acacgggaag 5520 aggggggttg tgctgaaggg ccaggaaggc
ggccattcgg gcaattcgct caacctcagg 5580 aacggagtag gtctcggtgt
cggaagcgac gccagatccg tcatcctcct ttcgctctcc 5640 aaagtagata
cctccgacga gctctcggac aatgatgaag tcggtgccct caacgtttcg 5700
gatgggggag agatcggcga gcttgggcga cagcagctgg cagggtcgca ggttggcgta
5760 caggttcagg tcctttcgca gcttgaggag accctgctcg ggtcgcacgt
cggttcgtcc 5820 gtcgggagtg gtccatacgg tgttggcagc gcctccgaca
gcaccgagca taatagagtc 5880 agcctttcgg cagatgtcga gagtagcgtc
ggtgatgggc tcgccctcct tctcaatggc 5940 agctcctcca atgagtcggt
cctcaaacac aaactcggtg ccggaggcct cagcaacaga 6000 cttgagcacc
ttgacggcct cggcaatcac ctcggggcca cagaagtcgc cgccgagaag 6060
aacaatcttc ttggagtcag tcttggtctt cttagtttcg ggttccattg tggatgtgtg
6120 tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt ggctggcaaa
cgctcttgta 6180 tatatacgca cttttgcccg tgctatgtgg aagactaaac
ctccgaagat tgtgactcag 6240 gtagtgcggt atcggctagg gacccaaacc
ttgtcgatgc cgatagcgct atcgaacgta 6300 ccccagccgg ccgggagtat
gtcggagggg acatacgaga tcgtcaaggg tttgtggcca 6360 actggtattt
aaatgatgtc gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg 6420
tggagaaagg ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgt gcttggagat
6480 ggaagccggt agaaccgggc tgcttggggg gatttggggc cgctgggctc
caaagagggg 6540 taggcatttc gttggggtta cgtaattgcg gcatttgggt
cctgcgcgca tgtcccattg 6600 gtcagaatta gtccggatag gagacttatc
agccaatcac agcgccggat ccacctgtag 6660 gttgggttgg gtgggagcac
ccctccacag agtagagtca aacagcagca gcaacatgat 6720 agttgggggt
gtgcgtgtta aaggaaaaaa aagaagcttg ggttatattc ccgctctatt 6780
tagaggttgc gggatagacg ccgacggagg gcaatggcgc tatggaacct tgcggatatc
6840 catacgccgc ggcggactgc gtccgaacca gctccagcag cgttttttcc
gggccattga 6900 gccgactgcg accccgccaa cgtgtcttgg cccacgcact
catgtcatgt tggtgttggg 6960 aggccacttt ttaagtagca caaggcacct
agctcgcagc aaggtgtccg aaccaaagaa 7020 gcggctgcag tggtgcaaac
ggggcggaaa cggcgggaaa aagccacggg ggcacgaatt 7080 gaggcacgcc
ctcgaatttg agacgagtca cggccccatt cgcccgcgca atggctcgcc 7140
aacgcccggt cttttgcacc acatcaggtt accccaagcc aaacctttgt gttaaaaagc
7200 ttaacatatt ataccgaacg taggtttggg cgggcttgct ccgtctgtcc
aaggcaacat 7260 ttatataagg gtctgcatcg ccggctcaat tgaatctttt
ttcttcttct cttctctata 7320 ttcattcttg aattaaacac acatcaacca
tggctaccaa gcagccctac cagttcccta 7380 ctctgaccga gatcaagcga
tctcttccct ccgagtgctt tgaagcctcg gtccctctgt 7440 ccttgtacta
caccgtgcga atcgtcgcta ttgccgttgc tctggccttc ggactcaact 7500
acgctcgagc ccttcccgtg gtcgagtctc tgtgggcact cgacgctgcc ctttgttgcg
7560 gttacgttct gctccaaggc attgtcttct ggggattctt taccgtgggt
cacgatgctg 7620 gacatggtgc cttctctcga taccacctgc tcaactttgt
cgttggcacc tttatccact 7680 ccctcattct tactcccttc gagtcgtgga
agctcacaca tcgacaccat cacaagaaca 7740 ccggaaacat cgaccgagac
gaaatcttct accctcagcg aaaggccgac gatcatcctc 7800 tgtctcgaaa
cctcgtcctg gctctcggtg ccgcttggtt tgcctacctt gtcgagggct 7860
ttcctccccg aaaggtcaac cacttcaacc ccttcgaacc tctgtttgtg cgacaggtgg
7920 ctgccgttgt catttccctc tctgctcact tcgccgtcct ggcactgtcc
gtgtatctga 7980 gctttcagtt cggtctcaag acaatggctc tgtactacta
tggacccgtc ttcgtgttcg 8040 gctccatgct cgtcattact acctttctgc
atcacaatga cgaggaaact ccttggtacg 8100 gagattccga ctggacctac
gtcaagggca acttgtcttc cgtggaccga tcttacggtg 8160 ccttcatcga
caacctctcg cacaacattg gcacacacca gatccaccat ctgtttccca 8220
tcattcctca ctacaagctc aaccgagcca ccgctgcctt ccaccaggcc tttcccgaac
8280 ttgtccgaaa gagcgacgag cccattctca aggctttctg gagagttggt
cgactttacg 8340 ccaactacgg agtcgtggat cccgacgcaa agctgtttac
tctcaaggag gccaaagctg 8400 cctccgaggc tgccaccaag accaaggcta
cttaagcggc cgcatgagaa gataaatata 8460 taaatacatt gagatattaa
atgcgctaga ttagagagcc tcatactgct cggagagaag 8520 ccaagacgag
tactcaaagg ggattacacc atccatatcc acagacacaa gctggggaaa 8580
ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg gggcagccag
8640 gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca
tcaagtcgta 8700 ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga
tgaagaatga acttgaagtg 8760 aaggaattta aatgtaacga aactgaaatt
tgaccagata ttgtgtccgc ggtggagctc 8820 cagcttttgt tccctttagt
gagggttaat ttcgagcttg gcgtaatcat ggtcatagct 8880 gtttcctgtg
tgaaattgtt atccgctcac aagcttccac acaac 8925 <210> SEQ ID NO
91 <211> LENGTH: 8925 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid p139 <400> SEQUENCE: 91 gtacgagccg
gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 60
ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
120 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
ttccgcttcc 180 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
gagcggtatc agctcactca 240 aaggcggtaa tacggttatc cacagaatca
ggggataacg caggaaagaa catgtgagca 300 aaaggccagc aaaaggccag
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 360 ctccgccccc
ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 420
acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
480 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
cgtggcgctt 540 tctcatagct cacgctgtag gtatctcagt tcggtgtagg
tcgttcgctc caagctgggc 600 tgtgtgcacg aaccccccgt tcagcccgac
cgctgcgcct tatccggtaa ctatcgtctt 660 gagtccaacc cggtaagaca
cgacttatcg ccactggcag cagccactgg taacaggatt 720 agcagagcga
ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 780
tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
840 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
tttttttgtt 900 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
aagatccttt gatcttttct 960 acggggtctg acgctcagtg gaacgaaaac
tcacgttaag ggattttggt catgagatta 1020 tcaaaaagga tcttcaccta
gatcctttta aattaaaaat gaagttttaa atcaatctaa 1080 agtatatatg
agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 1140
tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
1200 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
agacccacgc 1260 tcaccggctc cagatttatc agcaataaac cagccagccg
gaagggccga gcgcagaagt 1320 ggtcctgcaa ctttatccgc ctccatccag
tctattaatt gttgccggga agctagagta 1380 agtagttcgc cagttaatag
tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1440 tcacgctcgt
cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1500
acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
1560 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
taattctctt 1620 actgtcatgc catccgtaag atgcttttct gtgactggtg
agtactcaac caagtcattc 1680 tgagaatagt gtatgcggcg accgagttgc
tcttgcccgg cgtcaatacg ggataatacc 1740 gcgccacata gcagaacttt
aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1800 ctctcaagga
tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 1860
tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
1920 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
actcttcctt 1980 tttcaatatt attgaagcat ttatcagggt tattgtctca
tgagcggata catatttgaa 2040 tgtatttaga aaaataaaca aataggggtt
ccgcgcacat ttccccgaaa agtgccacct 2100 gacgcgccct gtagcggcgc
attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2160 gctacacttg
ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 2220
acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
2280 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
acgtagtggg 2340 ccatcgccct gatagacggt ttttcgccct ttgacgttgg
agtccacgtt ctttaatagt 2400 ggactcttgt tccaaactgg aacaacactc
aaccctatct cggtctattc ttttgattta 2460 taagggattt tgccgatttc
ggcctattgg ttaaaaaatg agctgattta acaaaaattt 2520 aacgcgaatt
ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca 2580
actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
2640 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
cgacgttgta 2700 aaacgacggc cagtgaattg taatacgact cactataggg
cgaattgggt accgggcccc 2760 ccctcgaggt cgatggtgtc gataagcttg
atatcgaatt catgtcacac aaaccgatct 2820 tcgcctcaag gaaacctaat
tctacatccg agagactgcc gagatccagt ctacactgat 2880 taattttcgg
gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat 2940
atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc
3000 gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa
taataaacag 3060 actccatcta ccgcctccaa atgatgttct caaaatatat
tgtatgaact tatttttatt 3120 acttagtatt attagacaac ttacttgctt
tatgaaaaac acttcctatt taggaaacaa 3180 tttataatgg cagttcgttc
atttaacaat ttatgtagaa taaatgttat aaatgcgtat 3240 gggaaatctt
aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca 3300
gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag
3360 aacagctatt cacacgttac tattgagatt attattggac gagaatcaca
cactcaactg 3420 tctttctctc ttctagaaat acaggtacaa gtatgtacta
ttctcattgt tcatacttct 3480 agtcatttca tcccacatat tccttggatt
tctctccaat gaatgacatt ctatcttgca 3540 aattcaacaa ttataataag
atataccaaa gtagcggtat agtggcaatc aaaaagcttc 3600 tctggtgtgc
ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt 3660
tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt attttgattt
3720 aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg
taggaaatta 3780 ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa
aatcgtattt ccaggttaga 3840 cgttccgcag aatctagaat gcggtatgcg
gtacattgtt cttcgaacgt aaaagttgcg 3900 ctccctgaga tattgtacat
ttttgctttt acaagtacaa gtacatcgta caactatgta 3960 ctactgttga
tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat 4020
gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca
4080 attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt
agcttatgca 4140 tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa
cggatgctca accgatttcg 4200 acagtaataa tttgaatcga atcggagcct
aaaatgaacc cgagtatatc tcataaaatt 4260 ctcggtgaga ggtctgtgac
tgtcagtaca aggtgccttc attatgccct caaccttacc 4320 atacctcact
gaatgtagtg tacctctaaa aatgaaatac agtgccaaaa gccaaggcac 4380
tgagctcgtc taacggactt gatatacaac caattaaaac aaatgaaaag aaatacagtt
4440 ctttgtatca tttgtaacaa ttaccctgta caaactaagg tattgaaatc
ccacaatatt 4500 cccaaagtcc acccctttcc aaattgtcat gcctacaact
catataccaa gcactaacct 4560 accaaacacc actaaaaccc cacaaaatat
atcttaccga atatacagta acaagctacc 4620 accacactcg ttgggtgcag
tcgccagctt aaagatatct atccacatca gccacaactc 4680 ccttccttta
ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta 4740
gacaagacac tttcaagaag actgtttcca aaacgtacca ctgtcctcca ctacaaacac
4800 acccaatctg cttcttctag tcaaggttgc tacaccggta aattataaat
catcatttca 4860 ttagcagggc agggcccttt ttatagagtc ttatacacta
gcggaccctg ccggtagacc 4920 aacccgcagg cgcgtcagtt tgctccttcc
atcaatgcgt cgtagaaacg acttactcct 4980 tcttgagcag ctccttgacc
ttgttggcaa caagtctccg acctcggagg tggaggaaga 5040 gcctccgata
tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac 5100
agcgtcaccg gcgggcttca tgttaagaga gaacttgagc atcatggcgg cagacagaat
5160 ggtggcaatg gggttgacct tctgcttgcc gagatcgggg gcagatccgt
gacagggctc 5220 gtacagaccg aacgcctcgt tggtgtcggg cagagaagcc
agagaggcgg agggcagcag 5280 acccagagaa ccggggatga cggaggcctc
gtcggagatg atatcgccaa acatgttggt 5340 ggtgatgatg ataccattca
tcttggaggg ctgcttgatg aggatcatgg cggccgagtc 5400 gatcagctgg
tggttgagct cgagctgggg gaattcgtcc ttgaggactc gagtgacagt 5460
ctttcgccaa agtcgagagg aggccagcac gttggccttg tcaagagacc acacgggaag
5520 aggggggttg tgctgaaggg ccaggaaggc ggccattcgg gcaattcgct
caacctcagg 5580 aacggagtag gtctcggtgt cggaagcgac gccagatccg
tcatcctcct ttcgctctcc 5640 aaagtagata cctccgacga gctctcggac
aatgatgaag tcggtgccct caacgtttcg 5700 gatgggggag agatcggcga
gcttgggcga cagcagctgg cagggtcgca ggttggcgta 5760 caggttcagg
tcctttcgca gcttgaggag accctgctcg ggtcgcacgt cggttcgtcc 5820
gtcgggagtg gtccatacgg tgttggcagc gcctccgaca gcaccgagca taatagagtc
5880 agcctttcgg cagatgtcga gagtagcgtc ggtgatgggc tcgccctcct
tctcaatggc 5940 agctcctcca atgagtcggt cctcaaacac aaactcggtg
ccggaggcct cagcaacaga 6000 cttgagcacc ttgacggcct cggcaatcac
ctcggggcca cagaagtcgc cgccgagaag 6060 aacaatcttc ttggagtcag
tcttggtctt cttagtttcg ggttccattg tggatgtgtg 6120 tggttgtatg
tgtgatgtgg tgtgtggagt gaaaatctgt ggctggcaaa cgctcttgta 6180
tatatacgca cttttgcccg tgctatgtgg aagactaaac ctccgaagat tgtgactcag
6240 gtagtgcggt atcggctagg gacccaaacc ttgtcgatgc cgatagcgct
atcgaacgta 6300 ccccagccgg ccgggagtat gtcggagggg acatacgaga
tcgtcaaggg tttgtggcca 6360 actggtattt aaatgatgtc gacgcagtag
gatgtcctgc acgggtcttt ttgtggggtg 6420 tggagaaagg ggtgcttgga
gatggaagcc ggtagaaccg ggctgcttgt gcttggagat 6480 ggaagccggt
agaaccgggc tgcttggggg gatttggggc cgctgggctc caaagagggg 6540
taggcatttc gttggggtta cgtaattgcg gcatttgggt cctgcgcgca tgtcccattg
6600 gtcagaatta gtccggatag gagacttatc agccaatcac agcgccggat
ccacctgtag 6660 gttgggttgg gtgggagcac ccctccacag agtagagtca
aacagcagca gcaacatgat 6720 agttgggggt gtgcgtgtta aaggaaaaaa
aagaagcttg ggttatattc ccgctctatt 6780 tagaggttgc gggatagacg
ccgacggagg gcaatggcgc tatggaacct tgcggatatc 6840 catacgccgc
ggcggactgc gtccgaacca gctccagcag cgttttttcc gggccattga 6900
gccgactgcg accccgccaa cgtgtcttgg cccacgcact catgtcatgt tggtgttggg
6960 aggccacttt ttaagtagca caaggcacct agctcgcagc aaggtgtccg
aaccaaagaa 7020 gcggctgcag tggtgcaaac ggggcggaaa cggcgggaaa
aagccacggg ggcacgaatt 7080 gaggcacgcc ctcgaatttg agacgagtca
cggccccatt cgcccgcgca atggctcgcc 7140 aacgcccggt cttttgcacc
acatcaggtt accccaagcc aaacctttgt gttaaaaagc 7200 ttaacatatt
ataccgaacg taggtttggg cgggcttgct ccgtctgtcc aaggcaacat 7260
ttatataagg gtctgcatcg ccggctcaat tgaatctttt ttcttcttct cttctctata
7320 ttcattcttg aattaaacac acatcaacca tggctaccaa gcagccctac
cagttcccta 7380 ctctgaccga gatcaagcga tctctgccct ccgagtgttt
cgaggcctcc gtgcctctct 7440 ctctgtacta caccgttcga tgcctggtca
ttgctgtgtc gctcgccttc ggacttcacc 7500 atgcacgatc tctgcccgtt
gtcgaaggcc tctgggctct ggatgccgct ctctgcaccg 7560 gttacgtgct
gctccagggc atcgtcttct ggggattctt tactgttggt cacgacgctg 7620
gacatggtgc cttctcccga taccacctgc tcaactttgt catcggaacc ttcattcact
7680 ctctcatcct tacacccttc gagtcctgga agctcaccca cagacaccat
cacaagaaca 7740 ctggcaacat cgaccgagac gaaatcttct accctcaacg
aaaggccgac gatcatcctc 7800 tgtctcgaaa cctcattctg gctttgggtg
cagcctggtt tgcctacctg gtcgaaggct 7860 ttcctccccg aaaggtcaac
cacttcaacc ccttcgagcc tctctttgtt cgacaggtct 7920 ctgccgtggt
catttcgctg gctgcgcact ttggagtggc tgccctgtcc atctacctca 7980
gcctgcagtt cggcttcaag actatggcca tctactacta tggtcccgtc tttgtgttcg
8040 gatccatgct cgtcattact acctttcttc atcacaacga cgaagagaca
ccttggtacg 8100 cagattcgga gtggacctac gtcaaaggca acctgtcctc
tgtcgaccga tcctacggtg 8160 ccctcatcga caacctttct cacaacatcg
gaacccacca gattcatcac ctctttccca 8220 tcattcctca ctacaagctc
aagcgagcta ccgaggcctt ccatcaagcc tttcccgagc 8280 tggttcgaaa
gtccgacgaa cccatcatca aggccttttt cagagtcggc cgactctacg 8340
caaactacgg tgtggtcgac tcggatgcca agctgttcac tctcaaggag gccaaggctg
8400 tttccgaagc cgctaccaag actaaggcca cctaagcggc cgcatgagaa
gataaatata 8460 taaatacatt gagatattaa atgcgctaga ttagagagcc
tcatactgct cggagagaag 8520 ccaagacgag tactcaaagg ggattacacc
atccatatcc acagacacaa gctggggaaa 8580 ggttctatat acactttccg
gaataccgta gtttccgatg ttatcaatgg gggcagccag 8640 gatttcaggc
acttcggtgt ctcggggtga aatggcgttc ttggcctcca tcaagtcgta 8700
ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga acttgaagtg
8760 aaggaattta aatgtaacga aactgaaatt tgaccagata ttgtgtccgc
ggtggagctc 8820 cagcttttgt tccctttagt gagggttaat ttcgagcttg
gcgtaatcat ggtcatagct 8880 gtttcctgtg tgaaattgtt atccgctcac
aagcttccac acaac 8925 <210> SEQ ID NO 92 <211> LENGTH:
8919 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pY140 <400> SEQUENCE: 92 gtacgagccg gaagcataaa
gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 60 ttaattgcgt
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 120
taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
180 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
agctcactca 240 aaggcggtaa tacggttatc cacagaatca ggggataacg
caggaaagaa catgtgagca 300 aaaggccagc aaaaggccag gaaccgtaaa
aaggccgcgt tgctggcgtt tttccatagg 360 ctccgccccc ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 420 acaggactat
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 480
ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
540 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
caagctgggc 600 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
tatccggtaa ctatcgtctt 660 gagtccaacc cggtaagaca cgacttatcg
ccactggcag cagccactgg taacaggatt 720 agcagagcga ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc 780 tacactagaa
ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 840
agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
900 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
gatcttttct 960 acggggtctg acgctcagtg gaacgaaaac tcacgttaag
ggattttggt catgagatta 1020 tcaaaaagga tcttcaccta gatcctttta
aattaaaaat gaagttttaa atcaatctaa 1080 agtatatatg agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc 1140 tcagcgatct
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 1200
acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
1260 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
gcgcagaagt 1320 ggtcctgcaa ctttatccgc ctccatccag tctattaatt
gttgccggga agctagagta 1380 agtagttcgc cagttaatag tttgcgcaac
gttgttgcca ttgctacagg catcgtggtg 1440 tcacgctcgt cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1500 acatgatccc
ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1560
agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
1620 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
caagtcattc 1680 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc 1740 gcgccacata gcagaacttt aaaagtgctc
atcattggaa aacgttcttc ggggcgaaaa 1800 ctctcaagga tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac 1860 tgatcttcag
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 1920
aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
1980 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
catatttgaa 2040 tgtatttaga aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct 2100 gacgcgccct gtagcggcgc attaagcgcg
gcgggtgtgg tggttacgcg cagcgtgacc 2160 gctacacttg ccagcgccct
agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 2220 acgttcgccg
gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 2280
agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
2340 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
ctttaatagt 2400 ggactcttgt tccaaactgg aacaacactc aaccctatct
cggtctattc ttttgattta 2460 taagggattt tgccgatttc ggcctattgg
ttaaaaaatg agctgattta acaaaaattt 2520 aacgcgaatt ttaacaaaat
attaacgctt acaatttcca ttcgccattc aggctgcgca 2580 actgttggga
agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 2640
gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
2700 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
accgggcccc 2760 ccctcgaggt cgatggtgtc gataagcttg atatcgaatt
catgtcacac aaaccgatct 2820 tcgcctcaag gaaacctaat tctacatccg
agagactgcc gagatccagt ctacactgat 2880 taattttcgg gccaataatt
taaaaaaatc gtgttatata atattatatg tattatatat 2940 atacatcatg
atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc 3000
gcctccaact gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag
3060 actccatcta ccgcctccaa atgatgttct caaaatatat tgtatgaact
tatttttatt 3120 acttagtatt attagacaac ttacttgctt tatgaaaaac
acttcctatt taggaaacaa 3180 tttataatgg cagttcgttc atttaacaat
ttatgtagaa taaatgttat aaatgcgtat 3240 gggaaatctt aaatatggat
agcataaatg atatctgcat tgcctaattc gaaatcaaca 3300 gcaacgaaaa
aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag 3360
aacagctatt cacacgttac tattgagatt attattggac gagaatcaca cactcaactg
3420 tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt
tcatacttct 3480 agtcatttca tcccacatat tccttggatt tctctccaat
gaatgacatt ctatcttgca 3540 aattcaacaa ttataataag atataccaaa
gtagcggtat agtggcaatc aaaaagcttc 3600 tctggtgtgc ttctcgtatt
tatttttatt ctaatgatcc attaaaggta tatatttatt 3660 tcttgttata
taatcctttt gtttattaca tgggctggat acataaaggt attttgattt 3720
aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta
3780 ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt
ccaggttaga 3840 cgttccgcag aatctagaat gcggtatgcg gtacattgtt
cttcgaacgt aaaagttgcg 3900 ctccctgaga tattgtacat ttttgctttt
acaagtacaa gtacatcgta caactatgta 3960 ctactgttga tgcatccaca
acagtttgtt ttgttttttt ttgttttttt tttttctaat 4020 gattcattac
cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca 4080
attaatcata gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca
4140 tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa cggatgctca
accgatttcg 4200 acagtaataa tttgaatcga atcggagcct aaaatgaacc
cgagtatatc tcataaaatt 4260 ctcggtgaga ggtctgtgac tgtcagtaca
aggtgccttc attatgccct caaccttacc 4320 atacctcact gaatgtagtg
tacctctaaa aatgaaatac agtgccaaaa gccaaggcac 4380 tgagctcgtc
taacggactt gatatacaac caattaaaac aaatgaaaag aaatacagtt 4440
ctttgtatca tttgtaacaa ttaccctgta caaactaagg tattgaaatc ccacaatatt
4500 cccaaagtcc acccctttcc aaattgtcat gcctacaact catataccaa
gcactaacct 4560 accaaacacc actaaaaccc cacaaaatat atcttaccga
atatacagta acaagctacc 4620 accacactcg ttgggtgcag tcgccagctt
aaagatatct atccacatca gccacaactc 4680 ccttccttta ataaaccgac
tacacccttg gctattgagg ttatgagtga atatactgta 4740 gacaagacac
tttcaagaag actgtttcca aaacgtacca ctgtcctcca ctacaaacac 4800
acccaatctg cttcttctag tcaaggttgc tacaccggta aattataaat catcatttca
4860 ttagcagggc agggcccttt ttatagagtc ttatacacta gcggaccctg
ccggtagacc 4920 aacccgcagg cgcgtcagtt tgctccttcc atcaatgcgt
cgtagaaacg acttactcct 4980 tcttgagcag ctccttgacc ttgttggcaa
caagtctccg acctcggagg tggaggaaga 5040 gcctccgata tcggcggtag
tgataccagc ctcgacggac tccttgacgg cagcctcaac 5100 agcgtcaccg
gcgggcttca tgttaagaga gaacttgagc atcatggcgg cagacagaat 5160
ggtggcaatg gggttgacct tctgcttgcc gagatcgggg gcagatccgt gacagggctc
5220 gtacagaccg aacgcctcgt tggtgtcggg cagagaagcc agagaggcgg
agggcagcag 5280 acccagagaa ccggggatga cggaggcctc gtcggagatg
atatcgccaa acatgttggt 5340 ggtgatgatg ataccattca tcttggaggg
ctgcttgatg aggatcatgg cggccgagtc 5400 gatcagctgg tggttgagct
cgagctgggg gaattcgtcc ttgaggactc gagtgacagt 5460 ctttcgccaa
agtcgagagg aggccagcac gttggccttg tcaagagacc acacgggaag 5520
aggggggttg tgctgaaggg ccaggaaggc ggccattcgg gcaattcgct caacctcagg
5580 aacggagtag gtctcggtgt cggaagcgac gccagatccg tcatcctcct
ttcgctctcc 5640 aaagtagata cctccgacga gctctcggac aatgatgaag
tcggtgccct caacgtttcg 5700 gatgggggag agatcggcga gcttgggcga
cagcagctgg cagggtcgca ggttggcgta 5760 caggttcagg tcctttcgca
gcttgaggag accctgctcg ggtcgcacgt cggttcgtcc 5820 gtcgggagtg
gtccatacgg tgttggcagc gcctccgaca gcaccgagca taatagagtc 5880
agcctttcgg cagatgtcga gagtagcgtc ggtgatgggc tcgccctcct tctcaatggc
5940 agctcctcca atgagtcggt cctcaaacac aaactcggtg ccggaggcct
cagcaacaga 6000 cttgagcacc ttgacggcct cggcaatcac ctcggggcca
cagaagtcgc cgccgagaag 6060 aacaatcttc ttggagtcag tcttggtctt
cttagtttcg ggttccattg tggatgtgtg 6120 tggttgtatg tgtgatgtgg
tgtgtggagt gaaaatctgt ggctggcaaa cgctcttgta 6180 tatatacgca
cttttgcccg tgctatgtgg aagactaaac ctccgaagat tgtgactcag 6240
gtagtgcggt atcggctagg gacccaaacc ttgtcgatgc cgatagcgct atcgaacgta
6300 ccccagccgg ccgggagtat gtcggagggg acatacgaga tcgtcaaggg
tttgtggcca 6360 actggtattt aaatgatgtc gacgcagtag gatgtcctgc
acgggtcttt ttgtggggtg 6420 tggagaaagg ggtgcttgga gatggaagcc
ggtagaaccg ggctgcttgt gcttggagat 6480 ggaagccggt agaaccgggc
tgcttggggg gatttggggc cgctgggctc caaagagggg 6540 taggcatttc
gttggggtta cgtaattgcg gcatttgggt cctgcgcgca tgtcccattg 6600
gtcagaatta gtccggatag gagacttatc agccaatcac agcgccggat ccacctgtag
6660 gttgggttgg gtgggagcac ccctccacag agtagagtca aacagcagca
gcaacatgat 6720 agttgggggt gtgcgtgtta aaggaaaaaa aagaagcttg
ggttatattc ccgctctatt 6780 tagaggttgc gggatagacg ccgacggagg
gcaatggcgc tatggaacct tgcggatatc 6840 catacgccgc ggcggactgc
gtccgaacca gctccagcag cgttttttcc gggccattga 6900 gccgactgcg
accccgccaa cgtgtcttgg cccacgcact catgtcatgt tggtgttggg 6960
aggccacttt ttaagtagca caaggcacct agctcgcagc aaggtgtccg aaccaaagaa
7020 gcggctgcag tggtgcaaac ggggcggaaa cggcgggaaa aagccacggg
ggcacgaatt 7080 gaggcacgcc ctcgaatttg agacgagtca cggccccatt
cgcccgcgca atggctcgcc 7140 aacgcccggt cttttgcacc acatcaggtt
accccaagcc aaacctttgt gttaaaaagc 7200 ttaacatatt ataccgaacg
taggtttggg cgggcttgct ccgtctgtcc aaggcaacat 7260 ttatataagg
gtctgcatcg ccggctcaat tgaatctttt ttcttcttct cttctctata 7320
ttcattcttg aattaaacac acatcaacca tggcttcctc taccgttgcc gctccctacg
7380 agttccctac tctcaccgag atcaagcgat ccctgcctgc ccactgcttc
gaagcctctg 7440 ttccctggtc cctctactat accgtgcgag ctctgggcat
tgccggttcc cttgctctcg 7500 gactgtacta tgctcgagcc cttgctatcg
tgcaggagtt tgcactgctc gatgccgtcc 7560 tttgcactgg ctacattctg
ctccagggta tcgtgttctg gggattcttt accatcggtc 7620 acgactgtgg
acatggtgcc ttctcgcgat cccacctgct caacttctct gttggcacac 7680
tcattcactc catcattctg actccctacg agtcgtggaa gatcagccat cgacaccatc
7740 acaagaacac cggcaacatc gacaaggatg agatcttcta ccctcagcga
gaagccgact 7800 ctcatcccct gtcccgacac atggtcatct cccttggttc
ggcttggttt gcctacctcg 7860 ttgctggatt tcctccccga aaggtcaacc
acttcaatcc ctgggagcct ctctacctgc 7920 gaagaatgtc tgccgtcatc
atttccctcg gctctctcgt ggcctttgct ggtctgtacg 7980 cctaccttac
ctacgtctac ggcctcaaga ccatggctct gtattacttc gcacctctct 8040
ttggattcgc caccatgctg gttgtcacta ccttcctcca tcacaacgac gaggaaactc
8100 cctggtacgc cgattcggag tggacctatg tcaagggcaa cttgtcctct
gtggaccgaa 8160 gctacggagc cctcatcgac aacctgtccc acaacattgg
tacacatcag atccaccatc 8220 tgtttcccat cattcctcac tacaagctca
acgaggccac tgctgccttc gctcaggcct 8280 ttcccgaact ggtgcgaaag
tcggcttctc ccatcattcc caccttcatc cgaattggtc 8340 ttatgtacgc
caagtacggc gtggtcgaca aggatgccaa gatgtttacc ctcaaggagg 8400
ccaaggctgc caagaccaaa gccaactaag cggccgcatg agaagataaa tatataaata
8460 cattgagata ttaaatgcgc tagattagag agcctcatac tgctcggaga
gaagccaaga 8520 cgagtactca aaggggatta caccatccat atccacagac
acaagctggg gaaaggttct 8580 atatacactt tccggaatac cgtagtttcc
gatgttatca atgggggcag ccaggatttc 8640 aggcacttcg gtgtctcggg
gtgaaatggc gttcttggcc tccatcaagt cgtaccatgt 8700 cttcatttgc
ctgtcaaagt aaaacagaag cagatgaaga atgaacttga agtgaaggaa 8760
tttaaatgta acgaaactga aatttgacca gatattgtgt ccgcggtgga gctccagctt
8820 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
agctgtttcc 8880 tgtgtgaaat tgttatccgc tcacaagctt ccacacaac 8919
<210> SEQ ID NO 93 <211> LENGTH: 6267 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY137 <400> SEQUENCE:
93 taactttggc cggcctttac ctgcaggata acttcgtata atgtatgcta
tacgaagtta 60 tgaattctgt aatattggga tctgttcgga aatcaacgga
tgctcaaccg atttcgacag 120 taataatttg aatcgaatcg gagcctaaaa
tgaacccgag tatatctcat aaaattctcg 180 gtgagaggtc tgtgactgtc
agtacaaggt gccttcatta tgccctcaac cttaccatac 240 ctcactgaat
gtagtgtacc tctaaaaatg aaatacagtg ccaaaagcca aggcactgag 300
ctcgtctaac ggacttgata tacaaccaat taaaacaaat gaaaagaaat acagttcttt
360 gtatcatttg taacaattac cctgtacaaa ctaaggtatt gaaatcccac
aatattccca 420 aagtccaccc ctttccaaat tgtcatgcct acaactcata
taccaagcac taacctacca 480 aacaccacta aaaccccaca aaatatatct
taccgaatat acagtaacaa gctaccacca 540 cactcgttgg gtgcagtcgc
cagcttaaag atatctatcc acatcagcca caactccctt 600 cctttaataa
accgactaca cccttggcta ttgaggttat gagtgaatat actgtagaca 660
agacactttc aagaagactg tttccaaaac gtaccactgt cctccactac aaacacaccc
720 aatctgcttc ttctagtcaa ggttgctaca ccggtaaatt ataaatcatc
atttcattag 780 cagggcaggg ccctttttat agagtcttat acactagcgg
accctgccgg tagaccaacc 840 cgcaggcgcg tcagtttgct ccttccatca
atgcgtcgta gaaacgactt actccttctt 900 gagcagctcc ttgaccttgt
tggcaacaag tctccgacct cggaggtgga ggaagagcct 960 ccgatatcgg
cggtagtgat accagcctcg acggactcct tgacggcagc ctcaacagcg 1020
tcaccggcgg gcttcatgtt aagagagaac ttgagcatca tggcggcaga cagaatggtg
1080 gcaatggggt tgaccttctg cttgccgaga tcgggggcag atccgtgaca
gggctcgtac 1140 agaccgaacg cctcgttggt gtcgggcaga gaagccagag
aggcggaggg cagcagaccc 1200 agagaaccgg ggatgacgga ggcctcgtcg
gagatgatat cgccaaacat gttggtggtg 1260 atgatgatac cattcatctt
ggagggctgc ttgatgagga tcatggcggc cgagtcgatc 1320 agctggtggt
tgagctcgag ctgggggaat tcgtccttga ggactcgagt gacagtcttt 1380
cgccaaagtc gagaggaggc cagcacgttg gccttgtcaa gagaccacac gggaagaggg
1440 gggttgtgct gaagggccag gaaggcggcc attcgggcaa ttcgctcaac
ctcaggaacg 1500 gagtaggtct cggtgtcgga agcgacgcca gatccgtcat
cctcctttcg ctctccaaag 1560 tagatacctc cgacgagctc tcggacaatg
atgaagtcgg tgccctcaac gtttcggatg 1620 ggggagagat cggcgagctt
gggcgacagc agctggcagg gtcgcaggtt ggcgtacagg 1680 ttcaggtcct
ttcgcagctt gaggagaccc tgctcgggtc gcacgtcggt tcgtccgtcg 1740
ggagtggtcc atacggtgtt ggcagcgcct ccgacagcac cgagcataat agagtcagcc
1800 tttcggcaga tgtcgagagt agcgtcggtg atgggctcgc cctccttctc
aatggcagct 1860 cctccaatga gtcggtcctc aaacacaaac tcggtgccgg
aggcctcagc aacagacttg 1920 agcaccttga cggcctcggc aatcacctcg
gggccacaga agtcgccgcc gagaagaaca 1980 atcttcttgg agtcagtctt
ggtcttctta gtttcgggtt ccattgtgga tgtgtgtggt 2040 tgtatgtgtg
atgtggtgtg tggagtgaaa atctgtggct ggcaaacgct cttgtatata 2100
tacgcacttt tgcccgtgct atgtggaaga ctaaacctcc gaagattgtg actcaggtag
2160 tgcggtatcg gctagggacc caaaccttgt cgatgccgat agcgctatcg
aacgtacccc 2220 agccggccgg gagtatgtcg gaggggacat acgagatcgt
caagggtttg tggccaactg 2280 gtatttaaat gatgtcgact catcgatata
acttcgtata atgtatgcta tacgaagtta 2340 tcctaggtat agatctgtta
ccggacagaa gtaccccaag ctcaacaaat gggctgtcaa 2400 ccacttcaac
cccaacgccc cgctgtttga gaagaaggac tggttcaaca tctggatctc 2460
taacgtcggt attggtatca ccatgtccgt catcgcatac tccatcaacc gatggggcct
2520 ggcttccgtc accctctact acctgatccc ctacctgtgg gtcaaccact
ggctcgtggc 2580 catcacctac ctgcagcaca ccgaccccac tctgccccac
taccacgccg accagtggaa 2640 cttcacccga ggagccgccg ccaccatcga
ccgagagttt ggcttcatcg gctccttctg 2700 cttccatgac atcatcgaga
cccacgttct gcaccactac gtgtctcgaa ttcccttcta 2760 caacgcccga
atcgccactg agaagatcaa gaaggtcatg ggcaagcact accgacacga 2820
cgacaccaac ttcatcaagt ctctttacac tgtcgcccga acctgccagt ttgttgaagg
2880 taaggaaggc attcagatgt ttagaaacgt caatggagtc ggagttgctc
ctgacggcct 2940 gccttctaaa ggcgcgccag ctgcattaat gaatcggcca
acgcgcgggg agaggcggtt 3000 tgcgtattgg gcgctcttcc gcttcctcgc
tcactgactc gctgcgctcg gtcgttcggc 3060 tgcggcgagc ggtatcagct
cactcaaagg cggtaatacg gttatccaca gaatcagggg 3120 ataacgcagg
aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3180
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
3240 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
tttccccctg 3300 gaagctccct cgtgcgctct cctgttccga ccctgccgct
taccggatac ctgtccgcct 3360 ttctcccttc gggaagcgtg gcgctttctc
atagctcacg ctgtaggtat ctcagttcgg 3420 tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3480 gcgccttatc
cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3540
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
3600 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
atctgcgctc 3660 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
ttgatccggc aaacaaacca 3720 ccgctggtag cggtggtttt tttgtttgca
agcagcagat tacgcgcaga aaaaaaggat 3780 ctcaagaaga tcctttgatc
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3840 gttaagggat
tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 3900
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3960 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
tccatagttg 4020 cctgactccc cgtcgtgtag ataactacga tacgggaggg
cttaccatct ggccccagtg 4080 ctgcaatgat accgcgagac ccacgctcac
cggctccaga tttatcagca ataaaccagc 4140 cagccggaag ggccgagcgc
agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4200 ttaattgttg
ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4260
ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
4320 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
aaagcggtta 4380 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
cgcagtgtta tcactcatgg 4440 ttatggcagc actgcataat tctcttactg
tcatgccatc cgtaagatgc ttttctgtga 4500 ctggtgagta ctcaaccaag
tcattctgag aatagtgtat gcggcgaccg agttgctctt 4560 gcccggcgtc
aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4620
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
4680 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
accagcgttt 4740 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
gggaataagg gcgacacgga 4800 aatgttgaat actcatactc ttcctttttc
aatattattg aagcatttat cagggttatt 4860 gtctcatgag cggatacata
tttgaatgta tttagaaaaa taaacaaata ggggttccgc 4920 gcacatttcc
ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg 4980
agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
5040 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
ccttataaat 5100 caaaagaata gaccgagata gggttgagtg ttgttccagt
ttggaacaag agtccactat 5160 taaagaacgt ggactccaac gtcaaagggc
gaaaaaccgt ctatcagggc gatggcccac 5220 tacgtgaacc atcaccctaa
tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 5280 ggaaccctaa
agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 5340
gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
5400 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
gcgtccattc 5460 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
cgggcctctt cgctattacg 5520 ccagctggcg aaagggggat gtgctgcaag
gcgattaagt tgggtaacgc cagggttttc 5580 ccagtcacga cgttgtaaaa
cgacggccag tgaattgtaa tacgactcac tatagggcga 5640 attgggcccg
acgtcgcatg catggattcg accacgcaga ccaacaccgg caccggcaag 5700
gtggccgtgc agccccccac ggccttcatt aagcccattg agaaggtgtc cgagcccgtc
5760 tacgacacct ttggcaacga gttcactcct ccagactact ctatcaagga
tattctggat 5820 gccattcccc aggagtgcta caagcggtcc tacgttaagt
cctactcgta cgtggcccga 5880 gactgcttct ttatcgccgt ttttgcctac
atggcctacg cgtacctgcc tcttattccc 5940 tcggcttccg gccgagctgt
ggcctgggcc atgtactcca ttgtccaggg tctgtttggc 6000 accggtctgt
gggttcttgc ccacgagtgt ggccactctg ctttctccga ctctaacacc 6060
gtcaacaacg tcaccggatg ggttctgcac tcctccatgc tggtccctta ctacgcctgg
6120 aagctgaccc actccatgca ccacaagtcc actggtcacc tcacccgtga
tatggtgttt 6180 gtgcccaagg accgaaagga gtttatggag aaccgaggcg
cccatgactg gtctgagctt 6240 gctgaggacg ctcccctcat gattaat 6267
<210> SEQ ID NO 94 <211> LENGTH: 9570 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY117 <400> SEQUENCE:
94 ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc
gggtggacgt 60 ctagaggtac ctagcaatta acagatagtt tgccggtgat
aattctctta acctcccaca 120 ctcctttgac ataacgattt atgtaacgaa
actgaaattt gaccagatat tgtgtccgcg 180 gtggagctcc agcttttgtt
ccctttagtg agggtttaaa cgagcttggc gtaatcatgg 240 tcatagctgt
ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc 300
ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
360 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
ttaatgaatc 420 ggccaacgcg cggggagagg cggtttgcgt attgggcgct
cttccgcttc ctcgctcact 480 gactcgctgc gctcggtcgt tcggctgcgg
cgagcggtat cagctcactc aaaggcggta 540 atacggttat ccacagaatc
aggggataac gcaggaaaga acatgtgagc aaaaggccag 600 caaaaggcca
ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 660
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
720 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
tccgaccctg 780 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
gcgtggcgct ttctcatagc 840 tcacgctgta ggtatctcag ttcggtgtag
gtcgttcgct ccaagctggg ctgtgtgcac 900 gaaccccccg ttcagcccga
ccgctgcgcc ttatccggta actatcgtct tgagtccaac 960 ccggtaagac
acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1020
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
1080 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
aagagttggt 1140 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
gtttttttgt ttgcaagcag 1200 cagattacgc gcagaaaaaa aggatctcaa
gaagatcctt tgatcttttc tacggggtct 1260 gacgctcagt ggaacgaaaa
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 1320 atcttcacct
agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1380
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
1440 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
tacgatacgg 1500 gagggcttac catctggccc cagtgctgca atgataccgc
gagacccacg ctcaccggct 1560 ccagatttat cagcaataaa ccagccagcc
ggaagggccg agcgcagaag tggtcctgca 1620 actttatccg cctccatcca
gtctattaat tgttgccggg aagctagagt aagtagttcg 1680 ccagttaata
gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1740
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
1800 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
cagaagtaag 1860 ttggccgcag tgttatcact catggttatg gcagcactgc
ataattctct tactgtcatg 1920 ccatccgtaa gatgcttttc tgtgactggt
gagtactcaa ccaagtcatt ctgagaatag 1980 tgtatgcggc gaccgagttg
ctcttgcccg gcgtcaatac gggataatac cgcgccacat 2040 agcagaactt
taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 2100
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
2160 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
aaatgccgca 2220 aaaaagggaa taagggcgac acggaaatgt tgaatactca
tactcttcct ttttcaatat 2280 tattgaagca tttatcaggg ttattgtctc
atgagcggat acatatttga atgtatttag 2340 aaaaataaac aaataggggt
tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 2400 tgtagcggcg
cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2460
gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
2520 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
tagtgcttta 2580 cggcacctcg accccaaaaa acttgattag ggtgatggtt
cacgtagtgg gccatcgccc 2640 tgatagacgg tttttcgccc tttgacgttg
gagtccacgt tctttaatag tggactcttg 2700 ttccaaactg gaacaacact
caaccctatc tcggtctatt cttttgattt ataagggatt 2760 ttgccgattt
cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 2820
tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg
2880 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
ggatgtgctg 2940 caaggcgatt aagttgggta acgccagggt tttcccagtc
acgacgttgt aaaacgacgg 3000 ccagtgaatt gtaatacgac tcactatagg
gcgaattggg taccgggccc cccctcgagg 3060 tcgatggtgt cgataagctt
gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa 3120 ggaaacctaa
ttctacatcc gagagactgc cgagatccag tctacactga ttaattttcg 3180
ggccaataat ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat
3240 gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc
cgcctccaac 3300 tgatgttctc aatatttaag gggtcatctc gcattgttta
ataataaaca gactccatct 3360 accgcctcca aatgatgttc tcaaaatata
ttgtatgaac ttatttttat tacttagtat 3420 tattagacaa cttacttgct
ttatgaaaaa cacttcctat ttaggaaaca atttataatg 3480 gcagttcgtt
catttaacaa tttatgtaga ataaatgtta taaatgcgta tgggaaatct 3540
taaatatgga tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa
3600 aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa
gaacagctat 3660 tcacacgtta ctattgagat tattattgga cgagaatcac
acactcaact gtctttctct 3720 cttctagaaa tacaggtaca agtatgtact
attctcattg ttcatacttc tagtcatttc 3780 atcccacata ttccttggat
ttctctccaa tgaatgacat tctatcttgc aaattcaaca 3840 attataataa
gatataccaa agtagcggta tagtggcaat caaaaagctt ctctggtgtg 3900
cttctcgtat ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat
3960 ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt
taattttttg 4020 cttaaattca atcccccctc gttcagtgtc aactgtaatg
gtaggaaatt accatacttt 4080 tgaagaagca aaaaaaatga aagaaaaaaa
aaatcgtatt tccaggttag acgttccgca 4140 gaatctagaa tgcggtatgc
ggtacattgt tcttcgaacg taaaagttgc gctccctgag 4200 atattgtaca
tttttgcttt tacaagtaca agtacatcgt acaactatgt actactgttg 4260
atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta
4320 ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc
aattaatcat 4380 agacttatga atctgcacgg tgtgcgctgc gagttacttt
tagcttatgc atgctacttg 4440 ggtgtaatat tgggatctgt tcggaaatca
acggatgctc aaccgatttc gacagtaatt 4500 aattaattcc ctagtcccag
tgtacacccg ccgatatcgc ttaccctgca gccggattaa 4560 ggttggcaat
ttttcacgtc cttgtctccg caattactca ccgggtggtt tataagattg 4620
caagcgtctt gatttgtctc tgtatactaa catgcaatcg cgactcgccc gacgggccac
4680 taacctggcc agaatctcca gatccaagta ttctcttggt ctgcgatatg
tttccaacac 4740 aaaagcccct gctgcccagc cggcaactgc tgagtgagta
ttccttgcca taaacgaccc 4800 agaaccactg tatagtgttt ggaagcacta
gtcagaagac cagcgaaaac aggtggaaaa 4860 aactgagacg aaaagcaacg
accagaaatg taatgtgtgg aaaagcgaca cacacagagc 4920 agataaagag
gtgacaaata acgacaaatg aaatatcagt atcttcccac aatcactacc 4980
tctcagctgt ctgaaggtgc ggctgatata tccatcccac gtctaacgta tggagtgtga
5040 tagaatatga cgacacaagc atgagaactc gctctctatc caaccaccga
aacactgtca 5100 ctacagccgt tcttgttgct ccattcgctt ttgtgattcc
atgccttctc tggtgactga 5160 caacattcct tccttttctc cagccctgtt
gttatctgct catgacctac ggccactctc 5220 tatcgcatac taacatagac
gatcccagcc cgctccccac ttccagggca ccgttggcaa 5280 gcctcctatc
ctcaagaagg ctgaggctgc caacgctgac atggacgagt ccttcatcgg 5340
aatgtctgga ggagagatct tccacgagat gatgctgcga cacaacgtcg acactgtctt
5400 cggttacccc ggtggagcca ttctccccgt ctttgacgcc attcacaact
ctgagtactt 5460 caactttgtg ctccctcgac acgagcaggg tgccggccac
atggccgagg gctacgctcg 5520 agcctctggt aagcccggtg tcgttctcgt
cacctctggc cccggtgcca ccaacgtcat 5580 cacccccatg caggacgctc
tttccgatgg tacccccatg gttgtcttca ccggtcaggt 5640 cctgacctcc
gttatcggca ctgacgcctt ccaggaggcc gatgttgtcg gcatctcccg 5700
atcttgcacc aagtggaacg tcatggtcaa gaacgttgct gagctccccc gacgaatcaa
5760 cgaggccttt gagattgcta cttccggccg acccggtccc gttctcgtcg
atctgcccaa 5820 ggatgttact gctgccatcc tgcgagagcc catccccacc
aagtccacca ttccctcgca 5880 ttctctgacc aacctcacct ctgccgccgc
caccgagttc cagaagcagg ctatccagcg 5940 agccgccaac ctcatcaacc
agtccaagaa gcccgtcctt tacgtcggac agggtatcct 6000 tggctccgag
gagggtccta agctgcttaa ggagctggct gagaaggccg agattcccgt 6060
caccactact ctgcagggtc ttggtgcctt tgacgagcga gaccccaagt ctctgcacat
6120 gctcggtatg cacggttccg gctacgccaa catggccatg cagaacgctg
actgtatcat 6180 tgctctcggc gcccgatttg atgaccgagt taccggctcc
atccccaagt ttgcccccga 6240 ggctcgagcc gctgcccttg agggtcgagg
tggtattgtt cactttgaga tccaggccaa 6300 gaacatcaac aaggttgttc
aggccaccga agccgttgag ggagacgtta ccgagtctgt 6360 ccgacagctc
atccccctca tcaacaaggt ctctgccgct gagcgagctc cctggactga 6420
gactatccag tcctggaagc agcagttccc cttcctcttc gaggctgaag gtgaggatgg
6480 tgttatcaag ccccagtccg tcattgctct gctctctgac ctgacagaga
acaacaagga 6540 caagaccatc atcaccaccg gtgttggtca gcatcagatg
tggactgccc agcatttccg 6600 atggcgacac cctcgaacca tgatcacttc
tggtggtctt ggaactatgg gttacggcct 6660 gcccgccgct atcggcgcca
aggttgcccg acctgactgc gacgtcattg acatcgatgg 6720 tgacgcttct
ttcaacatga ctctgaccga gctgtccacc gccgttcagt tcaacattgg 6780
cgtcaaggct attgtcctca acaacgagga acagggtatg gtcacccagc tgcagtctct
6840 cttctacgag aaccgatact gccacactca tcagaagaac cccgacttca
tgaagctggc 6900 cgagtccatg ggcatgaagg gtatccgaat cactcacatt
gaccagctgg aggccggtct 6960 caaggagatg ctcgcataca agggccctgt
gctcgttgag gttgttgtcg acaagaagat 7020 ccccgttctt cccatggttc
ccgctggtaa ggctttgcat gagttccttg tctacgacgc 7080 tgacgccgag
gctgcttctc gacccgatcg actgaagaat gcccccgccc ctcacgtcca 7140
ccagaccacc tttgagaact aagtggaaag gaacacaagc aatccgaacc aaaaataatt
7200 ggggtcccgt gcccacagag tctagtgcag acctaaaatg accacagtaa
attatagctg 7260 ttattaaaca tgagattttg accaacaaga gcgtaggaat
gttattagct actacttgta 7320 catacacagc atttgtttta aataatgttg
cctccagggg cagtgagatc aggacccaga 7380 tccgtggcca gctctctgac
ttcagaccgc ttgtacttaa gcagctcgca acactgttgt 7440 cgaggattga
acttgccata ttcgattttg tggtcatgaa tccagcacac ctcatttaaa 7500
tgtagctaac ggtagcaggc gaactactgg tacatacctc ccccggaata tgtacaggca
7560 taatgcgtat ctgtgggaca tgtggtcgtt gcgccattat gtaagcagcg
tgtactcctc 7620 tgactgtcca tatggtttgc tccatctcac cctcatcgtt
ttcattgttc acaggcggcc 7680 acaaaaaaac tgtcttctct ccttctctct
tcgccttagt ctactcggac cagttttagt 7740 ttagcttggc gccactggat
aaatgagacc tcaggccttg tgatgaggag gtcacttatg 7800 aagcatgtta
ggaggtgctt gtatggatag agaagcaccc aaaataataa gaataataat 7860
aaaacagggg gcgttgtcat ttcatatcgt gttttcacca tcaatacacc tccaaacaat
7920 gcccttcatg tggccagccc caatattgtc ctgtagttca actctatgca
gctcgtatct 7980 tattgagcaa gtaaaactct gtcagccgat attgcccgac
ccgcgacaag ggtcaacaag 8040 gtggtgtaag gccttcgcag aagtcaaaac
tgtgccaaac aaacatctag agtctctttg 8100 gtgtttctcg catatatttw
atcggctgtc ttacgtattt gcgcctcggt accggactaa 8160 tttcggatca
tccccaatac gctttttctt cgcagctgtc aacagtgtcc atgatctatc 8220
cacctaaatg ggtcatatga ggcgtataat ttcgtggtgc tgataataat tcccatatat
8280 ttgacacaaa acttcccccc ctagacatac atctcacaat ctcacttctt
gtgcttctgt 8340 cacacatctc ctccagctga cttcaactca cacctctgcc
ccagttggtc tacagcggta 8400 taaggtttct ccgcatagag gtgcaccact
cctcccgata cttgtttgtg tgacttgtgg 8460 gtcacgacat atatatctac
acacattgcg ccaccctttg gttcttccag cacaacaaaa 8520 acacgacacg
ctaaccatgg ccaatttact gaccgtacac caaaatttgc ctgcattacc 8580
ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
8640 ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
gggcggcatg 8700 gtgcaagttg aataaccgga aatggtttcc cgcagaacct
gaagatgttc gcgattatct 8760 tctatatctt caggcgcgcg gtctggcagt
aaaaactatc cagcaacatt tgggccagct 8820 aaacatgctt catcgtcggt
ccgggctgcc acgaccaagt gacagcaatg ctgtttcact 8880 ggttatgcgg
cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct 8940
agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg
9000 ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt
tacgtatagc 9060 cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
gacggtggga gaatgttaat 9120 ccatattggc agaacgaaaa cgctggttag
caccgcaggt gtagagaagg cacttagcct 9180 gggggtaact aaactggtcg
agcgatggat ttccgtctct ggtgtagctg atgatccgaa 9240 taactacctg
ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca 9300
gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc
9360 taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
gtgtcggagc 9420 cgcgcgagat atggcccgcg ctggagtttc aataccggag
atcatgcaag ctggtggctg 9480 gaccaatgta aatattgtca tgaactatat
ccgtaacctg gatagtgaaa caggggcaat 9540 ggtgcgcctg ctggaagatg
gcgattaagc 9570 <210> SEQ ID NO 95 <211> LENGTH: 358
<212> TYPE: PRT <213> ORGANISM: Saprolegnia diclina
<400> SEQUENCE: 95 Met Thr Glu Asp Lys Thr Lys Val Glu Phe
Pro Thr Leu Thr Glu Leu 1 5 10 15 Lys His Ser Ile Pro Asn Ala Cys
Phe Glu Ser Asn Leu Gly Leu Ser 20 25 30 Leu Tyr Tyr Thr Ala Arg
Ala Ile Phe Asn Ala Ser Ala Ser Ala Ala 35 40 45 Leu Leu Tyr Ala
Ala Arg Ser Thr Pro Phe Ile Ala Asp Asn Val Leu 50 55 60 Leu His
Ala Leu Val Cys Ala Thr Tyr Ile Tyr Val Gln Gly Val Ile 65 70 75 80
Phe Trp Gly Phe Phe Thr Val Gly His Asp Cys Gly His Ser Ala Phe 85
90 95 Ser Arg Tyr His Ser Val Asn Phe Ile Ile Gly Cys Ile Met His
Ser 100 105 110 Ala Ile Leu Thr Pro Phe Glu Ser Trp Arg Val Thr His
Arg His His 115 120 125 His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu
Ile Phe Tyr Pro His 130 135 140 Arg Ser Val Lys Asp Leu Gln Asp Val
Arg Gln Trp Val Tyr Thr Leu 145 150 155 160 Gly Gly Ala Trp Phe Val
Tyr Leu Lys Val Gly Tyr Ala Pro Arg Thr 165 170 175 Met Ser His Phe
Asp Pro Trp Asp Pro Leu Leu Leu Arg Arg Ala Ser 180 185 190 Ala Val
Ile Val Ser Leu Gly Val Trp Ala Ala Phe Phe Ala Ala Tyr 195 200 205
Ala Tyr Leu Thr Tyr Ser Leu Gly Phe Ala Val Met Gly Leu Tyr Tyr 210
215 220 Tyr Ala Pro Leu Phe Val Phe Ala Ser Phe Leu Val Ile Thr Thr
Phe 225 230 235 240 Leu His His Asn Asp Glu Ala Thr Pro Trp Tyr Gly
Asp Ser Glu Trp 245 250 255 Thr Tyr Val Lys Gly Asn Leu Ser Ser Val
Asp Arg Ser Tyr Gly Ala 260 265 270 Phe Val Asp Asn Leu Ser His His
Ile Gly Thr His Gln Val His His 275 280 285 Leu Phe Pro Ile Ile Pro
His Tyr Lys Leu Asn Glu Ala Thr Lys His 290 295 300 Phe Ala Ala Ala
Tyr Pro His Leu Val Arg Arg Asn Asp Glu Pro Ile 305 310 315 320 Ile
Thr Ala Phe Phe Lys Thr Ala His Leu Phe Val Asn Tyr Gly Ala 325 330
335 Val Pro Glu Thr Ala Gln Ile Phe Thr Leu Lys Glu Ser Ala Ala Ala
340 345 350 Ala Lys Ala Lys Ser Asp 355 <210> SEQ ID NO 96
<211> LENGTH: 9 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Delta-17 Desaturase Motif #1 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (3)..(3)
<223> OTHER INFORMATION: Xaa can be any naturally occurring
amino acid <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (7)..(7) <223> OTHER INFORMATION: Xaa
can be any naturally occurring amino acid <400> SEQUENCE: 96
Phe Thr Xaa Gly His Asp Xaa Gly His 1 5 <210> SEQ ID NO 97
<211> LENGTH: 9 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Delta-17 Desaturase Motif #2 <400> SEQUENCE: 97
His Arg His His His Lys Asn Thr Gly 1 5 <210> SEQ ID NO 98
<211> LENGTH: 11 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Delta-17 Desaturase Motif #3 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (6)..(6)
<223> OTHER INFORMATION: Xaa can be any naturally occurring
amino acid <400> SEQUENCE: 98 Ile Gly Thr His Gln Xaa His His
Leu Phe Pro 1 5 10 <210> SEQ ID NO 99 <211> LENGTH: 5
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: His-rich motif
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(4) <223> OTHER INFORMATION: Xaa can be any
naturally occurring amino acid <400> SEQUENCE: 99 His Xaa Xaa
Xaa His 1 5 <210> SEQ ID NO 100 <211> LENGTH: 5
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: His-rich motif
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(3) <223> OTHER INFORMATION: Xaa can be any
naturally occurring amino acid <400> SEQUENCE: 100 His Xaa
Xaa His His 1 5 <210> SEQ ID NO 101 <211> LENGTH: 5
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: His-rich motif
<220> FEATURE: <221> NAME/KEY: MISC_FEATURE <222>
LOCATION: (1)..(1) <223> OTHER INFORMATION: Xaa = His [H] or
Gln [Q] <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (2)..(3) <223> OTHER INFORMATION: Xaa
can be any naturally occurring amino acid <400> SEQUENCE: 101
Xaa Xaa Xaa His His 1 5 <210> SEQ ID NO 102 <211>
LENGTH: 8067 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pFBAINPaD17S <400> SEQUENCE: 102 ggccgcaagt
gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa 60
gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac
120 ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag
tacaatacta 180 aacatactgt acatactcat actcgtaccc gggcaacggt
ttcacttgag tgcagtggct 240 agtgctctta ctcgtacagt gtgcaatact
gcgtatcata gtctttgatg tatatcgtat 300 tcattcatgt tagttgcgta
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 360 gagtgagcta
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 420
tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
480 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
ctgcggcgag 540 cggtatcagc tcactcaaag gcggtaatac ggttatccac
agaatcaggg gataacgcag 600 gaaagaacat gtgagcaaaa ggccagcaaa
aggccaggaa ccgtaaaaag gccgcgttgc 660 tggcgttttt ccataggctc
cgcccccctg acgagcatca caaaaatcga cgctcaagtc 720 agaggtggcg
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 780
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
840 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
gtgtaggtcg 900 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
gcccgaccgc tgcgccttat 960 ccggtaacta tcgtcttgag tccaacccgg
taagacacga cttatcgcca ctggcagcag 1020 ccactggtaa caggattagc
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 1080 ggtggcctaa
ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 1140
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
1200 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag 1260 atcctttgat cttttctacg gggtctgacg ctcagtggaa
cgaaaactca cgttaaggga 1320 ttttggtcat gagattatca aaaaggatct
tcacctagat ccttttaaat taaaaatgaa 1380 gttttaaatc aatctaaagt
atatatgagt aaacttggtc tgacagttac caatgcttaa 1440 tcagtgaggc
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1500
ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
1560 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa 1620 gggccgagcg cagaagtggt cctgcaactt tatccgcctc
catccagtct attaattgtt 1680 gccgggaagc tagagtaagt agttcgccag
ttaatagttt gcgcaacgtt gttgccattg 1740 ctacaggcat cgtggtgtca
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1800 aacgatcaag
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1860
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
1920 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
actggtgagt 1980 actcaaccaa gtcattctga gaatagtgta tgcggcgacc
gagttgctct tgcccggcgt 2040 caatacggga taataccgcg ccacatagca
gaactttaaa agtgctcatc attggaaaac 2100 gttcttcggg gcgaaaactc
tcaaggatct taccgctgtt gagatccagt tcgatgtaac 2160 ccactcgtgc
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 2220
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
2280 tactcatact cttccttttt caatattatt gaagcattta tcagggttat
tgtctcatga 2340 gcggatacat atttgaatgt atttagaaaa ataaacaaat
aggggttccg cgcacatttc 2400 cccgaaaagt gccacctgac gcgccctgta
gcggcgcatt aagcgcggcg ggtgtggtgg 2460 ttacgcgcag cgtgaccgct
acacttgcca gcgccctagc gcccgctcct ttcgctttct 2520 tcccttcctt
tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 2580
ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
2640 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
acgttggagt 2700 ccacgttctt taatagtgga ctcttgttcc aaactggaac
aacactcaac cctatctcgg 2760 tctattcttt tgatttataa gggattttgc
cgatttcggc ctattggtta aaaaatgagc 2820 tgatttaaca aaaatttaac
gcgaatttta acaaaatatt aacgcttaca atttccattc 2880 gccattcagg
ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 2940
ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
3000 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
tatagggcga 3060 attgggtacc gggccccccc tcgaggtcga tggtgtcgat
aagcttgata tcgaattcat 3120 gtcacacaaa ccgatcttcg cctcaaggaa
acctaattct acatccgaga gactgccgag 3180 atccagtcta cactgattaa
ttttcgggcc aataatttaa aaaaatcgtg ttatataata 3240 ttatatgtat
tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata 3300
gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat
3360 tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa
aatatattgt 3420 atgaacttat ttttattact tagtattatt agacaactta
cttgctttat gaaaaacact 3480 tcctatttag gaaacaattt ataatggcag
ttcgttcatt taacaattta tgtagaataa 3540 atgttataaa tgcgtatggg
aaatcttaaa tatggatagc ataaatgata tctgcattgc 3600 ctaattcgaa
atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga 3660
aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag
3720 aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta
tgtactattc 3780 tcattgttca tacttctagt catttcatcc cacatattcc
ttggatttct ctccaatgaa 3840 tgacattcta tcttgcaaat tcaacaatta
taataagata taccaaagta gcggtatagt 3900 ggcaatcaaa aagcttctct
ggtgtgcttc tcgtatttat ttttattcta atgatccatt 3960 aaaggtatat
atttatttct tgttatataa tccttttgtt tattacatgg gctggataca 4020
taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact
4080 gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga
aaaaaaaaat 4140 cgtatttcca ggttagacgt tccgcagaat ctagaatgcg
gtatgcggta cattgttctt 4200 cgaacgtaaa agttgcgctc cctgagatat
tgtacatttt tgcttttaca agtacaagta 4260 catcgtacaa ctatgtacta
ctgttgatgc atccacaaca gtttgttttg tttttttttg 4320 tttttttttt
ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc 4380
cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt
4440 tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg
aaatcaacgg 4500 atgctcaatc gatttcgaca gtaattaatt aagtcataca
caagtcagct ttcttcgagc 4560 ctcatataag tataagtagt tcaacgtatt
agcactgtac ccagcatctc cgtatcgaga 4620 aacacaacaa catgccccat
tggacagatc atgcggatac acaggttgtg cagtatcata 4680 catactcgat
cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg 4740
cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc
4800 ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat
aggatctcgg 4860 ttctggccgt acagacctcg gccgacaatt atgatatccg
ttccggtaga catgacatcc 4920 tcaacagttc ggtactgctg tccgagagcg
tctcccttgt cgtcaagacc caccccgggg 4980 gtcagaataa gccagtcctc
agagtcgccc ttaggtcggt tctgggcaat gaagccaacc 5040 acaaactcgg
ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc 5100
agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg
5160 ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac
gtcctccttc 5220 ttctgttcag agacagtttc ctcggcacca gctcgcaggc
cagcaatgat tccggttccg 5280 ggtacaccgt gggcgttggt gatatcggac
cactcggcga ttcggtgaca ccggtactgg 5340 tgcttgacag tgttgccaat
atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc 5400 ttaagagcaa
gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg 5460
tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc
5520 tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
tgccacgagc 5580 ttgagcactc gagcggcaaa ggcggacttg tggacgttag
ctcgagcttc gtaggagggc 5640 attttggtgg tgaagaggag actgaaataa
atttagtctg cagaactttt tatcggaacc 5700 ttatctgggg cagtgaagta
tatgttatgg taatagttac gagttagttg aacttataga 5760 tagactggac
tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg 5820
tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata
5880 ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc
caacgaagaa 5940 tgtatcgtca aagtgatcca agcacactca tagttggagt
cgtactccaa aggcggcaat 6000 gacgagtcag acagatactc gtcgaaaaca
gtgtacgcag atctactata gaggaacatt 6060 taaattgccc cggagaagac
ggccaggccg cctagatgac aaattcaaca actcacagct 6120 gactttctgc
cattgccact aggggggggc ctttttatat ggccaagcca agctctccac 6180
gtcggttggg ctgcacccaa caataaatgg gtagggttgc accaacaaag ggatgggatg
6240 gggggtagaa gatacgagga taacggggct caatggcaca aataagaacg
aatactgcca 6300 ttaagactcg tgatccagcg actgacacca ttgcatcatc
taagggcctc aaaactacct 6360 cggaactgct gcgctgatct ggacaccaca
gaggttccga gcactttagg ttgcaccaaa 6420 tgtcccacca ggtgcaggca
gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa 6480 agtgagggcg
ctgaggtcga gcagggtggt gtgacttgtt atagccttta gagctgcgaa 6540
agcgcgtatg gatttggctc atcaggccag attgagggtc tgtggacaca tgtcatgtta
6600 gtgtacttca atcgccccct ggatatagcc ccgacaatag gccgtggcct
catttttttg 6660 ccttccgcac atttccattg ctcggtaccc acaccttgct
tctcctgcac ttgccaacct 6720 taatactggt ttacattgac caacatctta
caagcggggg gcttgtctag ggtatatata 6780 aacagtggct ctcccaatcg
gttgccagtc tcttttttcc tttctttccc cacagattcg 6840 aaatctaaac
tacacatcac agaattccga gccgtgagta tccacgacaa gatcagtgtc 6900
gagacgacgc gttttgtgta atgacacaat ccgaaagtcg ctagcaacac acactctcta
6960 cacaaactaa cccagctctg gtaccatggc ttcctctacc gttgccgctc
cctacgagtt 7020 ccctactctc accgagatca agcgatccct gcctgcccac
tgcttcgaag cctctgttcc 7080 ctggtccctc tactataccg tgcgagctct
gggcattgcc ggttcccttg ctctcggact 7140 gtactatgct cgagcccttg
ctatcgtgca ggagtttgca ctgctcgatg ccgtcctttg 7200 cactggctac
attctgctcc agggtatcgt gttctgggga ttctttacca tcggtcacga 7260
ctgtggacat ggtgccttct cgcgatccca cctgctcaac ttctctgttg gcacactcat
7320 tcactccatc attctgactc cctacgagtc gtggaagatc agccatcgac
accatcacaa 7380 gaacaccggc aacatcgaca aggatgagat cttctaccct
cagcgagaag ccgactctca 7440 tcccctgtcc cgacacatgg tcatctccct
tggttcggct tggtttgcct acctcgttgc 7500 tggatttcct ccccgaaagg
tcaaccactt caatccctgg gagcctctct acctgcgaag 7560 aatgtctgcc
gtcatcattt ccctcggctc tctcgtggcc tttgctggtc tgtacgccta 7620
ccttacctac gtctacggcc tcaagaccat ggctctgtat tacttcgcac ctctctttgg
7680 attcgccacc atgctggttg tcactacctt cctccatcac aacgacgagg
aaactccctg 7740 gtacgccgat tcggagtgga cctatgtcaa gggcaacttg
tcctctgtgg accgaagcta 7800 cggagccctc atcgacaacc tgtcccacaa
cattggtaca catcagatcc accatctgtt 7860 tcccatcatt cctcactaca
agctcaacga ggccactgct gccttcgctc aggcctttcc 7920 cgaactggtg
cgaaagtcgg cttctcccat cattcccacc ttcatccgaa ttggtcttat 7980
gtacgccaag tacggcgtgg tcgacaagga tgccaagatg tttaccctca aggaggccaa
8040 ggctgccaag accaaagcca actaagc 8067
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 102
<210> SEQ ID NO 1 <211> LENGTH: 1080 <212> TYPE:
DNA <213> ORGANISM: Pythium aphanidermatum <400>
SEQUENCE: 1 atggcttctt ccactgttgc tgcgccgtac gagttcccga cgctgacgga
gatcaagcgc 60 tcgctgccag cgcactgctt tgaggcctcg gtcccgtggt
cgctctacta caccgtgcgc 120 gcgctgggca tcgccggctc gctcgcgctc
ggcctctact acgcgcgcgc gctcgcgatc 180 gtgcaggagt ttgccctgct
ggatgcggtg ctctgcacgg ggtacattct gctgcagggc 240 atcgtattct
gggggttctt caccatcggc catgactgcg gccacggcgc gttctcgcgt 300
tcgcacctgc tcaacttcag cgtcggcacg ctcattcact cgatcatcct cacgccgtac
360 gagtcatgga agatctcgca ccgccaccac cacaagaaca cgggcaacat
cgacaaggac 420 gagattttct acccgcagcg cgaggccgac tcgcacccac
tgtcccgaca catggtgatc 480 tcgctcggct cggcctggtt cgcgtacctc
gttgcgggct tccctcctcg caaggtgaac 540 cacttcaacc cttgggaacc
gttgtacctg cgccgcatgt ctgccgtcat catctcactc 600 ggctcgctcg
tggcgttcgc gggcttgtat gcgtatctca cctacgtcta tggccttaag 660
accatggcgc tgtactactt cgcccctctc tttgggttcg ccacgatgct cgtggtcact
720 acctttttgc accacaatga cgaggaaacg ccatggtacg ccgactcgga
gtggacgtac 780 gtcaagggca acctctcgtc cgtggaccgc tcgtacggcg
cgctcatcga caacctgagc 840 cacaacatcg gcacgcacca gatccaccac
ctgtttccga tcatcccgca ctacaagctg 900 aacgaggcga cggcagcgtt
cgcgcaggcg ttcccggagc tcgtgcgcaa gagcgcgtcg 960 ccgatcatcc
cgacgttcat ccgcatcggg ctcatgtacg ccaagtacgg cgtcgtggac 1020
aaggacgcca agatgtttac gctcaaggag gccaaggccg ccaagaccaa ggccaactag
1080 <210> SEQ ID NO 2 <211> LENGTH: 359 <212>
TYPE: PRT <213> ORGANISM: Pythium aphanidermatum <220>
FEATURE: <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: delta-17 desaturase <400> SEQUENCE: 2 Met Ala
Ser Ser Thr Val Ala Ala Pro Tyr Glu Phe Pro Thr Leu Thr 1 5 10 15
Glu Ile Lys Arg Ser Leu Pro Ala His Cys Phe Glu Ala Ser Val Pro 20
25 30 Trp Ser Leu Tyr Tyr Thr Val Arg Ala Leu Gly Ile Ala Gly Ser
Leu 35 40 45 Ala Leu Gly Leu Tyr Tyr Ala Arg Ala Leu Ala Ile Val
Gln Glu Phe 50 55 60 Ala Leu Leu Asp Ala Val Leu Cys Thr Gly Tyr
Ile Leu Leu Gln Gly 65 70 75 80 Ile Val Phe Trp Gly Phe Phe Thr Ile
Gly His Asp Cys Gly His Gly 85 90 95 Ala Phe Ser Arg Ser His Leu
Leu Asn Phe Ser Val Gly Thr Leu Ile 100 105 110 His Ser Ile Ile Leu
Thr Pro Tyr Glu Ser Trp Lys Ile Ser His Arg 115 120 125 His His His
Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu Ile Phe Tyr 130 135 140 Pro
Gln Arg Glu Ala Asp Ser His Pro Leu Ser Arg His Met Val Ile 145 150
155 160 Ser Leu Gly Ser Ala Trp Phe Ala Tyr Leu Val Ala Gly Phe Pro
Pro 165 170 175 Arg Lys Val Asn His Phe Asn Pro Trp Glu Pro Leu Tyr
Leu Arg Arg 180 185 190 Met Ser Ala Val Ile Ile Ser Leu Gly Ser Leu
Val Ala Phe Ala Gly 195 200 205 Leu Tyr Ala Tyr Leu Thr Tyr Val Tyr
Gly Leu Lys Thr Met Ala Leu 210 215 220 Tyr Tyr Phe Ala Pro Leu Phe
Gly Phe Ala Thr Met Leu Val Val Thr 225 230 235 240 Thr Phe Leu His
His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp Ser 245 250 255 Glu Trp
Thr Tyr Val Lys Gly Asn Leu Ser Ser Val Asp Arg Ser Tyr 260 265 270
Gly Ala Leu Ile Asp Asn Leu Ser His Asn Ile Gly Thr His Gln Ile 275
280 285 His His Leu Phe Pro Ile Ile Pro His Tyr Lys Leu Asn Glu Ala
Thr 290 295 300 Ala Ala Phe Ala Gln Ala Phe Pro Glu Leu Val Arg Lys
Ser Ala Ser 305 310 315 320 Pro Ile Ile Pro Thr Phe Ile Arg Ile Gly
Leu Met Tyr Ala Lys Tyr 325 330 335 Gly Val Val Asp Lys Asp Ala Lys
Met Phe Thr Leu Lys Glu Ala Lys 340 345 350 Ala Ala Lys Thr Lys Ala
Asn 355 <210> SEQ ID NO 3 <211> LENGTH: 359 <212>
TYPE: PRT <213> ORGANISM: Pythium aphanidermatum <220>
FEATURE: <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: delta-17 desaturase <220> FEATURE: <221>
NAME/KEY: MISC_FEATURE <222> LOCATION: (155)..(155)
<223> OTHER INFORMATION: Xaa = Ser or Pro <220>
FEATURE: <221> NAME/KEY: MISC_FEATURE <222> LOCATION:
(351)..(351) <223> OTHER INFORMATION: Xaa = Ala or Thr
<400> SEQUENCE: 3 Met Ala Ser Ser Thr Val Ala Ala Pro Tyr Glu
Phe Pro Thr Leu Thr 1 5 10 15 Glu Ile Lys Arg Ser Leu Pro Ala His
Cys Phe Glu Ala Ser Val Pro 20 25 30 Trp Ser Leu Tyr Tyr Thr Val
Arg Ala Leu Gly Ile Ala Gly Ser Leu 35 40 45 Ala Leu Gly Leu Tyr
Tyr Ala Arg Ala Leu Ala Ile Val Gln Glu Phe 50 55 60 Ala Leu Leu
Asp Ala Val Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly 65 70 75 80 Ile
Val Phe Trp Gly Phe Phe Thr Ile Gly His Asp Cys Gly His Gly 85 90
95 Ala Phe Ser Arg Ser His Leu Leu Asn Phe Ser Val Gly Thr Leu Ile
100 105 110 His Ser Ile Ile Leu Thr Pro Tyr Glu Ser Trp Lys Ile Ser
His Arg 115 120 125 His His His Lys Asn Thr Gly Asn Ile Asp Lys Asp
Glu Ile Phe Tyr 130 135 140 Pro Gln Arg Glu Ala Asp Ser His Pro Leu
Xaa Arg His Met Val Ile 145 150 155 160 Ser Leu Gly Ser Ala Trp Phe
Ala Tyr Leu Val Ala Gly Phe Pro Pro 165 170 175 Arg Lys Val Asn His
Phe Asn Pro Trp Glu Pro Leu Tyr Leu Arg Arg 180 185 190 Met Ser Ala
Val Ile Ile Ser Leu Gly Ser Leu Val Ala Phe Ala Gly 195 200 205 Leu
Tyr Ala Tyr Leu Thr Tyr Val Tyr Gly Leu Lys Thr Met Ala Leu 210 215
220 Tyr Tyr Phe Ala Pro Leu Phe Gly Phe Ala Thr Met Leu Val Val Thr
225 230 235 240 Thr Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr
Ala Asp Ser 245 250 255 Glu Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser
Val Asp Arg Ser Tyr 260 265 270 Gly Ala Leu Ile Asp Asn Leu Ser His
Asn Ile Gly Thr His Gln Ile 275 280 285 His His Leu Phe Pro Ile Ile
Pro His Tyr Lys Leu Asn Glu Ala Thr 290 295 300 Ala Ala Phe Ala Gln
Ala Phe Pro Glu Leu Val Arg Lys Ser Ala Ser 305 310 315 320 Pro Ile
Ile Pro Thr Phe Ile Arg Ile Gly Leu Met Tyr Ala Lys Tyr 325 330 335
Gly Val Val Asp Lys Asp Ala Lys Met Phe Thr Leu Lys Glu Xaa Lys 340
345 350 Ala Ala Lys Thr Lys Ala Asn 355 <210> SEQ ID NO 4
<211> LENGTH: 1080 <212> TYPE: DNA <213>
ORGANISM: Pythium aphanidermatum <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: syntthetic
delta-17 desaturase, codon-optimized for Yarrowia lipolytica
<400> SEQUENCE: 4 atggcttcct ctaccgttgc cgctccctac gagttcccta
ctctcaccga gatcaagcga 60 tccctgcctg cccactgctt cgaagcctct
gttccctggt ccctctacta taccgtgcga 120 gctctgggca ttgccggttc
ccttgctctc ggactgtact atgctcgagc ccttgctatc 180 gtgcaggagt
ttgcactgct cgatgccgtc ctttgcactg gctacattct gctccagggt 240
atcgtgttct ggggattctt taccatcggt cacgactgtg gacatggtgc cttctcgcga
300 tcccacctgc tcaacttctc tgttggcaca ctcattcact ccatcattct
gactccctac 360 gagtcgtgga agatcagcca tcgacaccat cacaagaaca
ccggcaacat cgacaaggat 420 gagatcttct accctcagcg agaagccgac
tctcatcccc tgtcccgaca catggtcatc 480 tcccttggtt cggcttggtt
tgcctacctc gttgctggat ttcctccccg aaaggtcaac 540 cacttcaatc
cctgggagcc tctctacctg cgaagaatgt ctgccgtcat catttccctc 600
ggctctctcg tggcctttgc tggtctgtac gcctacctta cctacgtcta cggcctcaag
660 accatggctc tgtattactt cgcacctctc tttggattcg ccaccatgct
ggttgtcact 720 accttcctcc atcacaacga cgaggaaact ccctggtacg
ccgattcgga gtggacctat 780 gtcaagggca acttgtcctc tgtggaccga
agctacggag ccctcatcga caacctgtcc 840 cacaacattg gtacacatca
gatccaccat ctgtttccca tcattcctca ctacaagctc 900 aacgaggcca
ctgctgcctt cgctcaggcc tttcccgaac tggtgcgaaa gtcggcttct 960
cccatcattc ccaccttcat ccgaattggt cttatgtacg ccaagtacgg cgtggtcgac
1020 aaggatgcca agatgtttac cctcaaggag gccaaggctg ccaagaccaa
agccaactaa 1080 <210> SEQ ID NO 5 <211> LENGTH: 614
<212> TYPE: DNA <213> ORGANISM: Pythium aphanidermatum
<400> SEQUENCE: 5 ttttgggggt tcttcaccgt cggccatgac tgcggccacg
gcgcgttctc gcgttcgcac 60 ctgctcaact tcagcgtcgg cacgctcatt
cactcgatca tcctcacgcc gtacgagtca 120 tggaagatct cgcaccgcca
ccaccacaag aacacgggca acatcgacaa ggacgagatt 180 ttctacccgc
agcgcgaggc cgactcgcac ccactgtccc gacacatggt gatctcgctc 240
ggctcggcct ggttcgcgta cctcgttgcg ggcttccctc ctcgcatggt gaaccacttc
300 aacccttggg aaccgttgta cctgcgccgc atgtctgccg tcatcatctc
actcggctcg 360 ctcgtggcgt tcgcgggctt gtatgcgtat ctcacctacg
tctatggcct taagaccatg 420 gcgctgtact acttcgcccc tctctttggg
ttcgccacga tgctcgtggt cactaccttt 480 ttgcaccaca atggcgagga
aacgccatgg tacgccgact cggagtggac gtacgtcaag 540 ggcaacctct
cgtccgtgga ccgctcgtac ggcgcgctca tcgacaacct gagccacaac 600
atcggcacgc acaa 614 <210> SEQ ID NO 6 <211> LENGTH: 739
<212> TYPE: DNA <213> ORGANISM: Pythium aphanidermatum
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (132)..(132) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 6 actatagggc acgcgtggtc gacggcccgg
gctggtatca aatacttttt ctaatttaat 60 atctacgaaa cgtttttttg
ctatgattgg cacctattca atgctcatga atctgatgat 120 agtatttgca
tnacttcatc ctctcttcca ttttatgctg actcaaacct ctttcgcgct 180
cggtttcaaa gggttacact actcgtgcgt ggtaccgagt gtaaccagca gcaaaaccgc
240 tccatacaac cgccaagtgt gaatgagggg cagacactgc gcgtgatctt
gttctatgcg 300 cagccagcca gtggaggtct ctcccgggcg tggacctcac
ttcagcttga gccgcggacc 360 gcgcagacca cccgacccgc acccgccatg
gcttcttcca ctgttgctgc gccgtacgag 420 ttcccgacgc tgacggagat
caagcgctcg ctgccagcgc actgctttga ggcctcggtc 480 ccgtggtcgc
tctactacac cgtgcgcgcg ctgggcatcg ccggctcgct cgcgctcggc 540
ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg ccctgctgga tgcggtgctc
600 tgcacggggt acattctgct gcagggcatc gtattctggg ggttcttcac
catcggccat 660 gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca
acttcagcgt cggcacgctc 720 attcactcga tcatcctca 739 <210> SEQ
ID NO 7 <211> LENGTH: 512 <212> TYPE: DNA <213>
ORGANISM: Pythium aphanidermatum <400> SEQUENCE: 7 ctgtactact
tcgcccctct ctttgggttc gccacgatgc tcgtggtcac tacctttttg 60
caccacaatg acgaggaaac gccatggtac gccgactcgg agtggacgta cgtcaagggc
120 aacctctcgt ccgtggaccg ctcgtacggc gcgctcattg acaacctgag
ccacaacatc 180 ggcacgcacc agatccacca cctgtttccg atcatcccgc
actacaagct gaacgaggcg 240 acggcagcgt tcgcgcaggc gttcccggag
ctcgtgcgca agagcgcgtc gccgatcatc 300 ccgacgttca tccgcatcgg
gctcatgtac gccaagtacg gcgtcgtgga caaggacgcc 360 aagatgttta
cgctcaagga ggccaaggcc gccaagacca aggccaacta ggcagaggca 420
aacaaggaag agaagttgtg tataggctcg taatgaacat gcgggttttt tgtttttwww
480 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 512 <210> SEQ ID NO 8
<211> LENGTH: 1533 <212> TYPE: DNA <213>
ORGANISM: Pythium aphanidermatum <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: delta-17
desaturase contig (CDS corresponds to nucleotides 388-1467)
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (132)..(132) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 8 actatagggc acgcgtggtc gacggcccgg
gctggtatca aatacttttt ctaatttaat 60 atctacgaaa cgtttttttg
ctatgattgg cacctattca atgctcatga atctgatgat 120 agtatttgca
tnacttcatc ctctcttcca ttttatgctg actcaaacct ctttcgcgct 180
cggtttcaaa gggttacact actcgtgcgt ggtaccgagt gtaaccagca gcaaaaccgc
240 tccatacaac cgccaagtgt gaatgagggg cagacactgc gcgtgatctt
gttctatgcg 300 cagccagcca gtggaggtct ctcccgggcg tggacctcac
ttcagcttga gccgcggacc 360 gcgcagacca cccgacccgc acccgccatg
gcttcttcca ctgttgctgc gccgtacgag 420 ttcccgacgc tgacggagat
caagcgctcg ctgccagcgc actgctttga ggcctcggtc 480 ccgtggtcgc
tctactacac cgtgcgcgcg ctgggcatcg ccggctcgct cgcgctcggc 540
ctctactacg cgcgcgcgct cgcgatcgtg caggagtttg ccctgctgga tgcggtgctc
600 tgcacggggt acattctgct gcagggcatc gtattctggg ggttcttcac
catcggccat 660 gactgcggcc acggcgcgtt ctcgcgttcg cacctgctca
acttcagcgt cggcacgctc 720 attcactcga tcatcctcac gccgtacgag
tcatggaaga tctcgcaccg ccaccaccac 780 aagaacacgg gcaacatcga
caaggacgag attttctacc cgcagcgcga ggccgactcg 840 cacccactgt
cccgacacat ggtgatctcg ctcggctcgg cctggttcgc gtacctcgtt 900
gcgggcttcc ctcctcgcat ggtgaaccac ttcaaccctt gggaaccgtt gtacctgcgc
960 cgcatgtctg ccgtcatcat ctcactcggc tcgctcgtgg cgttcgcggg
cttgtatgcg 1020 tatctcacct acgtctatgg ccttaagacc atggcgctgt
actacttcgc ccctctcttt 1080 gggttcgcca cgatgctcgt ggtcactacc
tttttgcacc acaatgrcga ggaaacgcca 1140 tggtacgccg actcggagtg
gacgtacgtc aagggcaacc tctcgtccgt ggaccgctcg 1200 tacggcgcgc
tcattgacaa cctgagccac aacatcggca cgcaccagat ccaccacctg 1260
tttccgatca tcccgcacta caagctgaac gaggcgacgg cagcgttcgc gcaggcgttc
1320 ccggagctcg tgcgcaagag cgcgtcgccg atcatcccga cgttcatccg
catcgggctc 1380 atgtacgcca agtacggcgt cgtggacaag gacgccaaga
tgtttacgct caaggaggcc 1440 aaggccgcca agaccaaggc caactaggca
gaggcaaaca aggaagagaa gttgtgtata 1500 ggctcgtaat gaacatgcgg
gttttttgtt ttt 1533 <210> SEQ ID NO 9 <211> LENGTH: 39
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: SMART IV
oligonucleotide <400> SEQUENCE: 9 aagcagtggt atcaacgcag
agtggccatt acggccggg 39 <210> SEQ ID NO 10 <211>
LENGTH: 59 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
CDSIII/3'PCR primer <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (28)..(57) <223> OTHER
INFORMATION: thymidine (dT); see BD Biosciences Clontech's SMART
cDNA technology <220> FEATURE: <221> NAME/KEY:
misc_feature <222> LOCATION: (59)..(59) <223> OTHER
INFORMATION: n is a, c, g, or t <400> SEQUENCE: 10 attctagagg
ccgaggcggc cgacatgttt tttttttttt tttttttttt tttttttvn 59
<210> SEQ ID NO 11 <211> LENGTH: 23 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5'-PCR primer <400> SEQUENCE:
11 aagcagtggt atcaacgcag agt 23 <210> SEQ ID NO 12
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F1 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (18)..(18)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 12 ttytggggnt tyttyacngt 20 <210> SEQ ID NO 13
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Translation of primer PD17-F1
<400> SEQUENCE: 13 Phe Trp Gly Phe Phe Thr Tyr 1 5
<210> SEQ ID NO 14 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-F2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (9)..(9)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(12)..(12) <223> OTHER INFORMATION: n is a, c, g, or t
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (15)..(15) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 14 ttcttyacng tnggncayga 20 <210>
SEQ ID NO 15 <211> LENGTH: 20 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-F3 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (9)..(9)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(12)..(12) <223> OTHER INFORMATION: n is a, c, g, or t
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (15)..(15) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 15 tttttyacng tnggncayga 20 <210>
SEQ ID NO 16 <211> LENGTH: 7 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Translation of primers PD17-F2 and
PD17-F3 <400> SEQUENCE: 16 Phe Phe Thr Val Gly His Asp 1 5
<210> SEQ ID NO 17 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-F4 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (3)..(3)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(9)..(9) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 17 acncaycgnc aycaycayaa 20 <210> SEQ
ID NO 18 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-F5 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (3)..(3) <223>
OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 18
acncayagrc aycaycayaa 20 <210> SEQ ID NO 19 <211>
LENGTH: 7 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Translation of primers PD17-F4 and PD17-F5 <400> SEQUENCE: 19
Thr His Arg His His His Lys 1 5 <210> SEQ ID NO 20
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F6 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 20 aaraayacng gnaayatyga 20 <210> SEQ ID NO 21
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-F7 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 21 aaraayacng gnaayataga 20 <210> SEQ ID NO 22
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primers PD17-F6 and PD17-F7 <400>
SEQUENCE: 22 Lys Asn Thr Gly Asn Ile Asp 1 5 <210> SEQ ID NO
23 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-R1 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (15)..(15) <223>
OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 23
tcrtcrttrt grtgnagraa 20 <210> SEQ ID NO 24 <211>
LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
PD17-R2 <400> SEQUENCE: 24 tcrtcrttrt grtgyaaraa 20
<210> SEQ ID NO 25 <211> LENGTH: 7 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Translation of primers PD17-R1 and
PD17-R2 <400> SEQUENCE: 25 Phe Leu His His Asn Asp Glu 1 5
<210> SEQ ID NO 26 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-R3 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (18)..(18)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 26 aaraargcyt tdatdatngg 20 <210> SEQ ID NO 27
<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Primer PD17-R4 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (18)..(18) <223>
OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 27
aaraaygcyt tdatdatngg 20 <210> SEQ ID NO 28 <211>
LENGTH: 7 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Translation of primers PD17-R3 and PD17-R4
<400> SEQUENCE: 28 Pro Ile Ile Lys Ala Phe Phe 1 5
<210> SEQ ID NO 29 <211> LENGTH: 20 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PD17-R5 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (6)..(6)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(9)..(9) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 29 ttrtgngtnc cdatrttatg 20 <210> SEQ
ID NO 30 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-R6 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (9)..(9)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 30 ttrtgngtnc cdatrttgtg 20 <210> SEQ ID NO 31
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primers PD17-R5 and PD17-R6 <400>
SEQUENCE: 31 His Asn Ile Gly Thr His Gln 1 5 <210> SEQ ID NO
32 <211> LENGTH: 20 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Primer PD17-R7 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223>
OTHER INFORMATION: n is a, c, g, or t <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12)
<223> OTHER INFORMATION: n is a, c, g, or t <400>
SEQUENCE: 32 ccyttnacrt angtccaytc 20 <210> SEQ ID NO 33
<211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Translation of primer PD17-R7 <400> SEQUENCE: 33
Glu Trp Thr Tyr Val Lys Gly 1 5 <210> SEQ ID NO 34
<211> LENGTH: 44 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Genome Walker adaptor-1 <400> SEQUENCE: 34
gtaatacgac tatagggcac gcgtggtcga cggcccgggc tggt 44 <210> SEQ
ID NO 35 <211> LENGTH: 8 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Genome Walker adaptor-2 <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION: (1)..(1)
<223> OTHER INFORMATION: 5' end is associated with a -PO4
group <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (8)..(8) <223> OTHER INFORMATION: 3'
end is associated with a -H2N group <400> SEQUENCE: 35
accagccc 8 <210> SEQ ID NO 36 <211> LENGTH: 22
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer
PUD17-5-1 <400> SEQUENCE: 36 aatctcgtcc ttgtcgatgt tg 22
<210> SEQ ID NO 37 <211> LENGTH: 22 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AP1 <400> SEQUENCE: 37
gtaatacgac tcactatagg gc 22 <210> SEQ ID NO 38 <211>
LENGTH: 22 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
PUD17-5-3 <400> SEQUENCE: 38 tgaggatgat cgagtgaatg ag 22
<210> SEQ ID NO 39 <211> LENGTH: 19 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer AP2 <400> SEQUENCE: 39
actatagggc acgcgtggt 19 <210> SEQ ID NO 40 <211>
LENGTH: 22 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Primer
PUD17-3-1 <400> SEQUENCE: 40 cacctacgtc tatggcctta ag 22
<210> SEQ ID NO 41 <211> LENGTH: 22 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PUD17-3-2 <400>
SEQUENCE: 41 ctgtactact tcgcccctct ct 22 <210> SEQ ID NO 42
<211> LENGTH: 1085 <212> TYPE: DNA <213>
ORGANISM: Phytophthora infestans <400> SEQUENCE: 42
atggcgacga aggaggcgta tgtgttcccc actctgacgg agatcaagcg gtcgctacct
60 aagactgttt cgaggcttcg gtgcctctgt cgctctacta caccgtgcgt
tgtctggtga 120 tcgcggtggc tctaaccttc ggtctcaact acgctcgcgc
tctgcccgag gtcgagagct 180 tctgggctct ggacgccgca ctctgcacgg
gctacatctt gctgcagggc atcgtgttct 240 ggggcttctt cacggtgggc
cacgatgccg gccacggcgc cttctcgcgc taccacctgc 300 ttaacttcgt
ggtgggcact ttcatgcact cgctcatcct cacgcccttc gagtcgtgga 360
agctcacgca ccgtcaccac cacaagaaca cgggcaacat tgaccgtgac gaggtcttct
420 acccgcaacg caaggccgac gaccacccgc tgtctcgcaa cctgattctg
gcgctcgggg 480 cagcgtggct cgcctatttg gtcgagggct tccctcctcg
taaggtcaac cacttcaacc 540 cgttcgagcc tctgttcgtg cgtcaggtgt
cagctgtggt aatctctctt ctcgcccact 600 tcttcgtggc cggactctcc
atctatctga gcctccagct gggccttaag acgatggcaa 660 tctactacta
tggacctgtt tttgtgttcg gcagcatgct ggtcattacc accttcctac 720
accacaatga tgaggagacc ccatggtacg ccgactcgga gtggacgtac gtcaagggca
780 acctctcgtc cgtggaccga tcgtacggcg cgctcattga caacctgagc
cacaacatcg 840 gcacgcacca gatccaccac cttttcccta tcattccgca
ctacaaactc aagaaagcca 900 ctgcggcctt ccaccaggct ttccctgagc
tcgtgcgcaa gagcgacgag ccaattatca 960 aggctttctt ccgggttgga
cgtctctacg caaactacgg cgttgtggac caggaggcga 1020 agctcttcac
gctaaaggaa gccaaggcgg cgaccgaggc ggcggccaag accaagtcca 1080 cgtaa
1085 <210> SEQ ID NO 43 <211> LENGTH: 361 <212>
TYPE: PRT <213> ORGANISM: Phytophthora infestans <300>
PUBLICATION INFORMATION: <302> TITLE: METHOD FOR PRODUCING
UNSATURATED Omega3 FATTY ACIDS IN TRANSGENIC ORGANISMS <308>
DATABASE ACCESSION NUMBER: CAJ30870 <309> DATABASE ENTRY
DATE: 2005-09-21
<310> PATENT DOCUMENT NUMBER: WO 2005083053 <311>
PATENT FILING DATE: 2005-02-23 <312> PUBLICATION DATE:
2005-09-09 <313> RELEVANT RESIDUES IN SEQ ID NO: (1)..(361)
<400> SEQUENCE: 43 Met Ala Thr Lys Glu Ala Tyr Val Phe Pro
Thr Leu Thr Glu Ile Lys 1 5 10 15 Arg Ser Leu Pro Lys Asp Cys Phe
Glu Ala Ser Val Pro Leu Ser Leu 20 25 30 Tyr Tyr Thr Val Arg Cys
Leu Val Ile Ala Val Ala Leu Thr Phe Gly 35 40 45 Leu Asn Tyr Ala
Arg Ala Leu Pro Glu Val Glu Ser Phe Trp Ala Leu 50 55 60 Asp Ala
Ala Leu Cys Thr Gly Tyr Ile Leu Leu Gln Gly Ile Val Phe 65 70 75 80
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 85
90 95 Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Met His Ser
Leu 100 105 110 Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg
His His His 115 120 125 Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Val
Phe Tyr Pro Gln Arg 130 135 140 Lys Ala Asp Asp His Pro Leu Ser Arg
Asn Leu Ile Leu Ala Leu Gly 145 150 155 160 Ala Ala Trp Leu Ala Tyr
Leu Val Glu Gly Phe Pro Pro Arg Lys Val 165 170 175 Asn His Phe Asn
Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ser Ala 180 185 190 Val Val
Ile Ser Leu Leu Ala His Phe Phe Val Ala Gly Leu Ser Ile 195 200 205
Tyr Leu Ser Leu Gln Leu Gly Leu Lys Thr Met Ala Ile Tyr Tyr Tyr 210
215 220 Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe
Leu 225 230 235 240 His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp
Ser Glu Trp Thr 245 250 255 Tyr Val Lys Gly Asn Leu Ser Ser Val Asp
Arg Ser Tyr Gly Ala Leu 260 265 270 Ile Asp Asn Leu Ser His Asn Ile
Gly Thr His Gln Ile His His Leu 275 280 285 Phe Pro Ile Ile Pro His
Tyr Lys Leu Lys Lys Ala Thr Ala Ala Phe 290 295 300 His Gln Ala Phe
Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Ile 305 310 315 320 Lys
Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 325 330
335 Asp Gln Glu Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Thr
340 345 350 Glu Ala Ala Ala Lys Thr Lys Ser Thr 355 360 <210>
SEQ ID NO 44 <211> LENGTH: 1092 <212> TYPE: DNA
<213> ORGANISM: Phytophthora sojae <400> SEQUENCE: 44
atggcgtcca agcaggagca gccgtaccag ttcccgacgc tgacggagat caagcgctcg
60 ctgcccagcg agtgtttcga ggcgtccgtg ccgctctcgc tctactacac
ggtgcgctgc 120 ctggtgatcg ccgtgtcgct ggccttcggg ctccaccacg
cgcgctcgct gcccgtggtc 180 gagggcctct gggcgctgga cgccgcgctc
tgcacgggct acgtgctgct gcagggcatc 240 gtgttctggg gcttcttcac
cgtgggccat gacgccggcc acggcgcctt ctcgcgctac 300 cacctgctca
acttcgtgat cggcaccttc atccactcgc tcatcctgac gcccttcgag 360
tcgtggaagc tcacgcaccg ccaccaccac aagaacacgg gcaacatcga ccgcgacgag
420 atcttctacc cgcagcgcaa ggccgacgac cacccgctct cgcgtaacct
catcctggcg 480 ctgggcgccg cgtggttcgc ctacctggtc gagggcttcc
cgccgcgcaa ggtcaaccac 540 ttcaacccgt tcgagccgct gttcgtccgc
caggtgtccg ccgtggtcat ctcgctggcc 600 gcgcacttcg gcgtggccgc
gctgtccatc tacctgagcc tgcagttcgg cttcaagacc 660 atggctatct
actactacgg gcccgtgttc gtgttcggca gcatgctggt catcaccacc 720
ttcctgcacc acaacgacga ggagaccccc tggtacgccg actcggagtg gacctacgtc
780 aagggcaacc tctcgtcggt cgaccgctcc tacggcgcgc tcatcgacaa
cctgagccac 840 aacatcggca cgcaccagat ccaccacctc ttccccatca
tcccgcacta taagctcaag 900 cgcgccaccg aggccttcca ccaggcgttc
cccgagctcg tgcgcaagag cgacgagccc 960 atcattaagg ccttcttccg
cgtcggccgc ctctacgcca actacggcgt cgtggactcg 1020 gacgccaagc
tcttcacgct caaggaggcc aaggccgtgt ccgaggcggc gaccaagact 1080
aaggccaact ga 1092 <210> SEQ ID NO 45 <211> LENGTH: 363
<212> TYPE: PRT <213> ORGANISM: Phytophthora sojae
<400> SEQUENCE: 45 Met Ala Ser Lys Gln Glu Gln Pro Tyr Gln
Phe Pro Thr Leu Thr Glu 1 5 10 15 Ile Lys Arg Ser Leu Pro Ser Glu
Cys Phe Glu Ala Ser Val Pro Leu 20 25 30 Ser Leu Tyr Tyr Thr Val
Arg Cys Leu Val Ile Ala Val Ser Leu Ala 35 40 45 Phe Gly Leu His
His Ala Arg Ser Leu Pro Val Val Glu Gly Leu Trp 50 55 60 Ala Leu
Asp Ala Ala Leu Cys Thr Gly Tyr Val Leu Leu Gln Gly Ile 65 70 75 80
Val Phe Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala 85
90 95 Phe Ser Arg Tyr His Leu Leu Asn Phe Val Ile Gly Thr Phe Ile
His 100 105 110 Ser Leu Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr
His Arg His 115 120 125 His His Lys Asn Thr Gly Asn Ile Asp Arg Asp
Glu Ile Phe Tyr Pro 130 135 140 Gln Arg Lys Ala Asp Asp His Pro Leu
Ser Arg Asn Leu Ile Leu Ala 145 150 155 160 Leu Gly Ala Ala Trp Phe
Ala Tyr Leu Val Glu Gly Phe Pro Pro Arg 165 170 175 Lys Val Asn His
Phe Asn Pro Phe Glu Pro Leu Phe Val Arg Gln Val 180 185 190 Ser Ala
Val Val Ile Ser Leu Ala Ala His Phe Gly Val Ala Ala Leu 195 200 205
Ser Ile Tyr Leu Ser Leu Gln Phe Gly Phe Lys Thr Met Ala Ile Tyr 210
215 220 Tyr Tyr Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr
Thr 225 230 235 240 Phe Leu His His Asn Asp Glu Glu Thr Pro Trp Tyr
Ala Asp Ser Glu 245 250 255 Trp Thr Tyr Val Lys Gly Asn Leu Ser Ser
Val Asp Arg Ser Tyr Gly 260 265 270 Ala Leu Ile Asp Asn Leu Ser His
Asn Ile Gly Thr His Gln Ile His 275 280 285 His Leu Phe Pro Ile Ile
Pro His Tyr Lys Leu Lys Arg Ala Thr Glu 290 295 300 Ala Phe His Gln
Ala Phe Pro Glu Leu Val Arg Lys Ser Asp Glu Pro 305 310 315 320 Ile
Ile Lys Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly 325 330
335 Val Val Asp Ser Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala
340 345 350 Val Ser Glu Ala Ala Thr Lys Thr Lys Ala Asn 355 360
<210> SEQ ID NO 46 <211> LENGTH: 1086 <212> TYPE:
DNA <213> ORGANISM: Phytophthora ramorum <400>
SEQUENCE: 46 atggcgacta agcagccgta ccagttcccg accctgacgg agatcaagcg
gtcgctgccc 60 agcgagtgct ttgaggcctc ggtgccgctg tcgctctact
acacggtgcg catcgtggcc 120 atcgccgtgg cgctggcgtt cggcctcaac
tacgcgcgcg cgctgcccgt ggtcgagagc 180 ttgtgggcgc tggacgctgc
gctctgctgc ggttacgtgc tgctgcaggg catcgtgttc 240 tggggcttct
tcacggtggg ccatgacgcc ggccacggcg ccttctcgcg ttaccacctg 300
ctcaacttcg tggtgggcac cttcatccac tcgctcatcc tcacgccctt cgagtcgtgg
360 aagctcacgc accgccacca ccacaagaac acgggcaaca ttgaccgcga
cgagatcttc 420 tacccgcagc gcaaggccga cgaccacccg ctgtcgcgca
acctcgtgct ggcgctcggc 480 gccgcgtggt tcgcctacct ggtcgagggc
ttcccgcccc gcaaggtcaa ccacttcaac 540 ccattcgagc cgctgtttgt
gcgccaggtg gccgccgtcg tcatctcgct ctccgcgcac 600 ttcgccgtgt
tggcgctgtc cgtgtatctg agcttccagt tcggtctcaa gaccatggcg 660
ctctactact acggccccgt cttcgtgttc ggcagcatgc ttgtgatcac caccttcctg
720 catcacaatg acgaggagac cccatggtac ggagactccg actggaccta
cgtcaagggc 780 aacctgtcgt ccgtggaccg gtcctacggc gcgttcatcg
acaacctgag ccacaacatc 840 ggcacgcacc agatccacca cctcttcccc
atcatcccgc actacaagct caaccgcgct 900 acggcggcat tccaccaggc
cttccccgag ctcgtgcgca agagcgacga gccgatcctc 960 aaggccttct
ggcgcgtcgg ccgactgtac gccaactacg gcgtcgtgga cccggacgcc 1020
aagctcttca cgctcaagga ggccaaggcg gcgtccgagg cggcgaccaa gaccaaggcc
1080 acctaa 1086 <210> SEQ ID NO 47 <211> LENGTH: 361
<212> TYPE: PRT <213> ORGANISM: Phytophthora
ramorum
<400> SEQUENCE: 47 Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro
Thr Leu Thr Glu Ile Lys 1 5 10 15 Arg Ser Leu Pro Ser Glu Cys Phe
Glu Ala Ser Val Pro Leu Ser Leu 20 25 30 Tyr Tyr Thr Val Arg Ile
Val Ala Ile Ala Val Ala Leu Ala Phe Gly 35 40 45 Leu Asn Tyr Ala
Arg Ala Leu Pro Val Val Glu Ser Leu Trp Ala Leu 50 55 60 Asp Ala
Ala Leu Cys Cys Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 65 70 75 80
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 85
90 95 Arg Tyr His Leu Leu Asn Phe Val Val Gly Thr Phe Ile His Ser
Leu 100 105 110 Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg
His His His 115 120 125 Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile
Phe Tyr Pro Gln Arg 130 135 140 Lys Ala Asp Asp His Pro Leu Ser Arg
Asn Leu Val Leu Ala Leu Gly 145 150 155 160 Ala Ala Trp Phe Ala Tyr
Leu Val Glu Gly Phe Pro Pro Arg Lys Val 165 170 175 Asn His Phe Asn
Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ala Ala 180 185 190 Val Val
Ile Ser Leu Ser Ala His Phe Ala Val Leu Ala Leu Ser Val 195 200 205
Tyr Leu Ser Phe Gln Phe Gly Leu Lys Thr Met Ala Leu Tyr Tyr Tyr 210
215 220 Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe
Leu 225 230 235 240 His His Asn Asp Glu Glu Thr Pro Trp Tyr Gly Asp
Ser Asp Trp Thr 245 250 255 Tyr Val Lys Gly Asn Leu Ser Ser Val Asp
Arg Ser Tyr Gly Ala Phe 260 265 270 Ile Asp Asn Leu Ser His Asn Ile
Gly Thr His Gln Ile His His Leu 275 280 285 Phe Pro Ile Ile Pro His
Tyr Lys Leu Asn Arg Ala Thr Ala Ala Phe 290 295 300 His Gln Ala Phe
Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Leu 305 310 315 320 Lys
Ala Phe Trp Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 325 330
335 Asp Pro Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Ala Ser
340 345 350 Glu Ala Ala Thr Lys Thr Lys Ala Thr 355 360 <210>
SEQ ID NO 48 <211> LENGTH: 7145 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pKUNFmkF2 <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(692)..(695) <223> OTHER INFORMATION: n is a, c, g, or t
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (700)..(700) <223> OTHER INFORMATION: n is a, c, g,
or t <400> SEQUENCE: 48 catggcgtcc acttcggctc tgcccaagca
gaaccctgcg cttagacgca ccgtcacctc 60 aactactgtg acggattctg
agtctgccgc cgtctctcct tcagactctc cccgccactc 120 ggcctcttcc
acatcgctct cgtccatgtc cgaggttgat atcgccaagc ccaagtccga 180
gtatggtgtc atgctcgaca cctacggcaa ccagttcgag gttcccgact ttaccatcaa
240 ggacatctac aatgccatcc ctaagcactg cttcaagcgc tccgctctca
agggatacgg 300 ttatatcctc cgcgacattg tcctcctgac taccactttc
agcatctggt acaactttgt 360 gacccccgaa tatatcccct ccacccccgc
ccgcgctggt ctgtgggccg tgtacaccgt 420 tcttcagggt cttttcggta
ctggtctctg ggttattgcc catgagtgcg gtcacggtgc 480 tttctccgat
tctcgcatca tcaacgacat tactggctgg gttcttcact cttccctcct 540
tgtcccctac ttcagctggc aaatctccca ccgaaagcac cacaaggcca ctggcaacat
600 ggagcgtgac atggtcttcg ttccccgaac ccgcgagcag caggctactc
gtctcggaaa 660 gatgacccac gagctcgctc atcttactga gnnnntcgtn
ggctggccca actacctcat 720 caccaatgtt accggccaca actaccacga
gcgccagcgt gagggtcgcg gcaagggcaa 780 gcataacggc ctcggcggtg
gtgttaacca cttcgatccc cgcagccctc tgtacgagaa 840 cagtgacgct
aagctcatcg tcctcagcga tattggtatc ggtctgatgg ccactgctct 900
gtacttcctc gttcagaagt tcggtttcta caacatggcc atctggtact ttgttcccta
960 cctctgggtt aaccactggc tcgttgccat caccttcctc cagcacaccg
accctaccct 1020 tccccactac accaacgacg agtggaactt cgtccgtggt
gccgctgcta ccattgaccg 1080 tgagatgggc ttcatcggcc gccaccttct
ccacggcatc atcgagactc atgtcctcca 1140 ccactacgtc agcagcatcc
ccttctacaa cgcggacgag gccaccgagg ccattaagcc 1200 catcatgggc
aagcactacc gggctgatgt ccaggatggt cctcgtggct tcatccgcgc 1260
catgtaccgc agtgcgcgta tgtgccagtg ggttgagccc agcgctggtg ccgagggtgc
1320 tggtaagggt gttctgttct tccgcaaccg caacaacgtg ggcacccccc
ccgctgttat 1380 caagcccgtt gcttaagtag gcgcggccgc tatttatcac
tctttacaac ttctacctca 1440 actatctact ttaataaatg aatatcgttt
attctctatg attactgtat atgcgttcct 1500 ctaagacaaa tcgaaaccag
catgtgatcg aatggcatac aaaagtttct tccgaagttg 1560 atcaatgtcc
tgatagtcag gcagcttgag aagattgaca caggtggagg ccgtagggaa 1620
ccgatcaacc tgtctaccag cgttacgaat ggcaaatgac gggttcaaag ccttgaatcc
1680 ttgcaatggt gccttggata ctgatgtcac aaacttaaga agcagccgct
tgtcctcttc 1740 ctcgatcgat ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac 1800 aacgtacgaa gtcgtcaatg atgtcgatat
gggttttgat catgcacaca taaggtccga 1860 ccttatcggc aagctcaatg
agctccttgg tggtggtaac atccagagaa gcacacaggt 1920 tggttttctt
ggctgccacg agcttgagca ctcgagcggc aaaggcggac ttgtggacgt 1980
tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa taaatttagt
2040 ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta
tggtaatagt 2100 tacgagttag ttgaacttat agatagactg gactatacgg
ctatcggtcc aaattagaaa 2160 gaacgtcaat ggctctctgg gcgtcgcctt
tgccgacaaa aatgtgatca tgatgaaagc 2220 cagcaatgac gttgcagctg
atattgttgt cggccaaccg cgccgaaaac gcagctgtca 2280 gacccacagc
ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg 2340
agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgacc ttttccttgg
2400 gaaccaccac cgtcagccct tctgactcac gtattgtagc caccgacaca
ggcaacagtc 2460 cgtggatagc agaatatgtc ttgtcggtcc atttctcacc
aactttaggc gtcaagtgaa 2520 tgttgcagaa gaagtatgtg ccttcattga
gaatcggtgt tgctgatttc aataaagtct 2580 tgagatcagt ttggcgcgcc
agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 2640 tttgcgtatt
gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 2700
gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
2760 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
accgtaaaaa 2820 ggccgcgttg ctggcgtttt tccataggct ccgcccccct
gacgagcatc acaaaaatcg 2880 acgctcaagt cagaggtggc gaaacccgac
aggactataa agataccagg cgtttccccc 2940 tggaagctcc ctcgtgcgct
ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3000 ctttctccct
tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 3060
ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
3120 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
acttatcgcc 3180 actggcagca gccactggta acaggattag cagagcgagg
tatgtaggcg gtgctacaga 3240 gttcttgaag tggtggccta actacggcta
cactagaaga acagtatttg gtatctgcgc 3300 tctgctgaag ccagttacct
tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 3360 caccgctggt
agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3420
atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
3480 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
tccttttaaa 3540 ttaaaaatga agttttaaat caatctaaag tatatatgag
taaacttggt ctgacagtta 3600 ccaatgctta atcagtgagg cacctatctc
agcgatctgt ctatttcgtt catccatagt 3660 tgcctgactc cccgtcgtgt
agataactac gatacgggag ggcttaccat ctggccccag 3720 tgctgcaatg
ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 3780
gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
3840 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
tgcgcaacgt 3900 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
tttggtatgg cttcattcag 3960 ctccggttcc caacgatcaa ggcgagttac
atgatccccc atgttgtgca aaaaagcggt 4020 tagctccttc ggtcctccga
tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4080 ggttatggca
gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4140
gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
4200 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
aagtgctcat 4260 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
ttaccgctgt tgagatccag 4320 ttcgatgtaa cccactcgtg cacccaactg
atcttcagca tcttttactt tcaccagcgt 4380 ttctgggtga gcaaaaacag
gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 4440 gaaatgttga
atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 4500
ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
4560 gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac
agatgcgtaa 4620 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
ttgttaaaat tcgcgttaaa 4680 tttttgttaa atcagctcat tttttaacca
ataggccgaa atcggcaaaa tcccttataa 4740 atcaaaagaa tagaccgaga
tagggttgag tgttgttcca gtttggaaca agagtccact 4800 attaaagaac
gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 4860
actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
4920 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
cgaacgtggc 4980 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
gcgctggcaa gtgtagcggt 5040 cacgctgcgc gtaaccacca cacccgccgc
gcttaatgcg ccgctacagg gcgcgtccat 5100 tcgccattca ggctgcgcaa
ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 5160 cgccagctgg
cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 5220
tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
5280 gaattgggcc cgacgtcgca tgcagtggtg gtattgtgac tggggatgta
gttgagaata 5340 agtcatacac aagtcagctt tcttcgagcc tcatataagt
ataagtagtt caacgtatta 5400 gcactgtacc cagcatctcc gtatcgagaa
acacaacaac atgccccatt ggacagatca 5460 tgcggataca caggttgtgc
agtatcatac atactcgatc agacaggtcg tctgaccatc 5520 atacaagctg
aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat 5580
atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt
5640 atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg
ccgacaatta 5700 tgatatccgt tccggtagac atgacatcct caacagttcg
gtactgctgt ccgagagcgt 5760 ctcccttgtc gtcaagaccc accccggggg
tcagaataag ccagtcctca gagtcgccct 5820 taattaattt gaatcgaatc
gatgagccta aaatgaaccc gagtatatct cataaaattc 5880 tcggtgagag
gtctgtgact gtcagtacaa ggtgccttca ttatgccctc aaccttacca 5940
tacctcactg aatgtagtgt acctctaaaa atgaaataca gtgccaaaag ccaaggcact
6000 gagctcgtct aacggacttg atatacaacc aattaaaaca aatgaaaaga
aatacagttc 6060 tttgtatcat ttgtaacaat taccctgtac aaactaaggt
attgaaatcc cacaatattc 6120 ccaaagtcca cccctttcca aattgtcatg
cctacaactc atataccaag cactaaccta 6180 ccgtttaaac agtgtacgca
gatctactat agaggaacat ttaaattgcc ccggagaaga 6240 cggccaggcc
gcctagatga caaattcaac aactcacagc tgactttctg ccattgccac 6300
tagggggggg cctttttata tggccaagcc aagctctcca cgtcggttgg gctgcaccca
6360 acaataaatg ggtagggttg caccaacaaa gggatgggat ggggggtaga
agatacgagg 6420 ataacggggc tcaatggcac aaataagaac gaatactgcc
attaagactc gtgatccagc 6480 gactgacacc attgcatcat ctaagggcct
caaaactacc tcggaactgc tgcgctgatc 6540 tggacaccac agaggttccg
agcactttag gttgcaccaa atgtcccacc aggtgcaggc 6600 agaaaacgct
ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg 6660
agcagggtgg tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct
6720 catcaggcca gattgagggt ctgtggacac atgtcatgtt agtgtacttc
aatcgccccc 6780 tggatatagc cccgacaata ggccgtggcc tcattttttt
gccttccgca catttccatt 6840 gctcgatacc cacaccttgc ttctcctgca
cttgccaacc ttaatactgg tttacattga 6900 ccaacatctt acaagcgggg
ggcttgtcta gggtatatat aaacagtggc tctcccaatc 6960 ggttgccagt
ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca 7020
cagaattccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt
7080 aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta
acccagctct 7140 ggtac 7145 <210> SEQ ID NO 49 <211>
LENGTH: 5473 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pDMW287F <400> SEQUENCE: 49 ggccgcattg atgattggaa
acacacacat gggttatatc taggtgagag ttagttggac 60 agttatatat
taaatcagct atgccaacgg taacttcatt catgtcaacg aggaaccagt 120
gactgcaagt aatatagaat ttgaccacct tgccattctc ttgcactcct ttactatatc
180 tcatttattt cttatataca aatcacttct tcttcccagc atcgagctcg
gaaacctcat 240 gagcaataac atcgtggatc tcgtcaatag agggcttttt
ggactccttg ctgttggcca 300 ccttgtcctt gctgtctggc tcattctgtt
tcaacgcctt ttaattaatc gagcttggcg 360 taatcatggt catagctgtt
tcctgtgtga aattgttatc cgctcacaat tccacacaac 420 atacgagccg
gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 480
ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
540 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
ttccgcttcc 600 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
gagcggtatc agctcactca 660 aaggcggtaa tacggttatc cacagaatca
ggggataacg caggaaagaa catgtgagca 720 aaaggccagc aaaaggccag
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 780 ctccgccccc
ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 840
acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
900 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
cgtggcgctt 960 tctcatagct cacgctgtag gtatctcagt tcggtgtagg
tcgttcgctc caagctgggc 1020 tgtgtgcacg aaccccccgt tcagcccgac
cgctgcgcct tatccggtaa ctatcgtctt 1080 gagtccaacc cggtaagaca
cgacttatcg ccactggcag cagccactgg taacaggatt 1140 agcagagcga
ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 1200
tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1260 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
tttttttgtt 1320 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
aagatccttt gatcttttct 1380 acggggtctg acgctcagtg gaacgaaaac
tcacgttaag ggattttggt catgagatta 1440 tcaaaaagga tcttcaccta
gatcctttta aattaaaaat gaagttttaa atcaatctaa 1500 agtatatatg
agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 1560
tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
1620 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
agacccacgc 1680 tcaccggctc cagatttatc agcaataaac cagccagccg
gaagggccga gcgcagaagt 1740 ggtcctgcaa ctttatccgc ctccatccag
tctattaatt gttgccggga agctagagta 1800 agtagttcgc cagttaatag
tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1860 tcacgctcgt
cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1920
acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
1980 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
taattctctt 2040 actgtcatgc catccgtaag atgcttttct gtgactggtg
agtactcaac caagtcattc 2100 tgagaatagt gtatgcggcg accgagttgc
tcttgcccgg cgtcaatacg ggataatacc 2160 gcgccacata gcagaacttt
aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 2220 ctctcaagga
tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 2280
tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
2340 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
actcttcctt 2400 tttcaatatt attgaagcat ttatcagggt tattgtctca
tgagcggata catatttgaa 2460 tgtatttaga aaaataaaca aataggggtt
ccgcgcacat ttccccgaaa agtgccacct 2520 gacgcgccct gtagcggcgc
attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2580 gctacacttg
ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 2640
acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
2700 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
acgtagtggg 2760 ccatcgccct gatagacggt ttttcgccct ttgacgttgg
agtccacgtt ctttaatagt 2820 ggactcttgt tccaaactgg aacaacactc
aaccctatct cggtctattc ttttgattta 2880 taagggattt tgccgatttc
ggcctattgg ttaaaaaatg agctgattta acaaaaattt 2940 aacgcgaatt
ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca 3000
actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
3060 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
cgacgttgta 3120 aaacgacggc cagtgaattg taatacgact cactataggg
cgaattgggt accgggcccc 3180 ccctcgaggt cgacgtttaa acagtgtacg
cagtactata gaggaacatc gattgccccg 3240 gagaagacgg ccaggccgcc
tagatgacaa attcaacaac tcacagctga ctttctgcca 3300 ttgccactag
gggggggcct ttttatatgg ccaagccaag ctctccacgt cggttgggct 3360
gcacccaaca ataaatgggt agggttgcac caacaaaggg atgggatggg gggtagaaga
3420 tacgaggata acggggctca atggcacaaa taagaacgaa tactgccatt
aagactcgtg 3480 atccagcgac tgacaccatt gcatcatcta agggcctcaa
aactacctcg gaactgctgc 3540 gctgatctgg acaccacaga ggttccgagc
actttaggtt gcaccaaatg tcccaccagg 3600 tgcaggcaga aaacgctgga
acagcgtgta cagtttgtct taacaaaaag tgagggcgct 3660 gaggtcgagc
agggtggtgt gacttgttat agcctttaga gctgcgaaag cgcgtatgga 3720
tttggctcat caggccagat tgagggtctg tggacacatg tcatgttagt gtacttcaat
3780 cgccccctgg atatagcccc gacaataggc cgtggcctca tttttttgcc
ttccgcacat 3840 ttccattgct cggtacccac accttgcttc tcctgcactt
gccaacctta atactggttt 3900 acattgacca acatcttaca agcggggggc
ttgtctaggg tatatataaa cagtggctct 3960 cccaatcggt tgccagtctc
ttttttcctt tctttcccca cagattcgaa atctaaacta 4020 cacatcacac
aatgcctgtt actgacgtcc ttaagcgaaa gtccggtgtc atcgtcggcg 4080
acgatgtccg agccgtgagt atccacgaca agatcagtgt cgagacgacg cgttttgtgt
4140 aatgacacaa tccgaaagtc gctagcaaca cacactctct acacaaacta
acccagctct 4200 ccatggtgaa gtccaagcga caggctctgc ccctcaccat
cgacggaact acctacgacg 4260 tctccgcttg ggtgaacttc caccctggtg
gagctgaaat cattgagaac taccagggac 4320 gagatgctac tgacgccttc
atggttatgc actctcagga agccttcgac aagctcaagc 4380 gaatgcccaa
gatcaacccc tcctccgagc tgcctcccca ggctgccgtc aacgaagctc 4440
aggaggattt ccgaaagctc cgagaagagc tgatcgccac tggcatgttt gacgcctctc
4500 ccctctggta ctcgtacaag atctccacca ccctgggtct tggcgtgctt
ggatacttcc 4560 tgatggtcca gtaccagatg tacttcattg gtgctgtgct
gctcggtatg cactaccagc 4620 aaatgggatg gctgtctcat gacatctgcc
accaccagac cttcaagaac cgaaactgga 4680 ataacctcgt gggtctggtc
tttggcaacg gactccaggg cttctccgtg acctggtgga 4740 aggacagaca
caacgcccat cattctgcta ccaacgttca gggtcacgat cccgacattg 4800
ataacctgcc tctgctcgcc tggtccgagg acgatgtcac tcgagcttct cccatctccc
4860 gaaagctcat tcagttccaa cagtactatt tcctggtcat ctgtattctc
ctgcgattca 4920
tctggtgttt ccagtctgtg ctgaccgttc gatccctcaa ggaccgagac aaccagttct
4980 accgatctca gtacaagaaa gaggccattg gactcgctct gcactggact
ctcaagaccc 5040 tgttccacct cttctttatg ccctccatcc tgacctcgct
cctggtgttc tttgtttccg 5100 agctcgtcgg tggcttcgga attgccatcg
tggtcttcat gaaccactac cctctggaga 5160 agatcggtga ttccgtctgg
gacggacatg gcttctctgt gggtcagatc catgagacca 5220 tgaacattcg
acgaggcatc attactgact ggttctttgg aggcctgaac taccagatcg 5280
agcaccatct ctggcccacc ctgcctcgac acaacctcac tgccgtttcc taccaggtgg
5340 aacagctgtg ccagaagcac aacctcccct accgaaaccc tctgccccat
gaaggtctcg 5400 tcatcctgct ccgatacctg gccgtgttcg ctcgaatggc
cgagaagcag cccgctggca 5460 aggctctcta agc 5473 <210> SEQ ID
NO 50 <211> LENGTH: 9513 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pDMW214 <400> SEQUENCE: 50
ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
60 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
aacatacgag 120 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
gagctaactc acattaattg 180 cgttgcgctc actgcccgct ttccagtcgg
gaaacctgtc gtgccagctg cattaatgaa 240 tcggccaacg cgcggggaga
ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 300 ctgactcgct
gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 360
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
420 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
aggctccgcc 480 cccctgacga gcatcacaaa aatcgacgct caagtcagag
gtggcgaaac ccgacaggac 540 tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc 600 tgccgcttac cggatacctg
tccgcctttc tcccttcggg aagcgtggcg ctttctcata 660 gctcacgctg
taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 720
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
780 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
attagcagag 840 cgaggtatgt aggcggtgct acagagttct tgaagtggtg
gcctaactac ggctacacta 900 gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg 960 gtagctcttg atccggcaaa
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 1020 agcagattac
gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 1080
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
1140 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
taaagtatat 1200 atgagtaaac ttggtctgac agttaccaat gcttaatcag
tgaggcacct atctcagcga 1260 tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac 1320 gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg 1380 ctccagattt
atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 1440
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
1500 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
gtgtcacgct 1560 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
atcaaggcga gttacatgat 1620 cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta 1680 agttggccgc agtgttatca
ctcatggtta tggcagcact gcataattct cttactgtca 1740 tgccatccgt
aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 1800
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
1860 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
aaactctcaa 1920 ggatcttacc gctgttgaga tccagttcga tgtaacccac
tcgtgcaccc aactgatctt 1980 cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg 2040 caaaaaaggg aataagggcg
acacggaaat gttgaatact catactcttc ctttttcaat 2100 attattgaag
catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 2160
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
2220 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
accgctacac 2280 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
ttcctttctc gccacgttcg 2340 ccggctttcc ccgtcaagct ctaaatcggg
ggctcccttt agggttccga tttagtgctt 2400 tacggcacct cgaccccaaa
aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 2460 cctgatagac
ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 2520
tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
2580 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
tttaacgcga 2640 attttaacaa aatattaacg cttacaattt ccattcgcca
ttcaggctgc gcaactgttg 2700 ggaagggcga tcggtgcggg cctcttcgct
attacgccag ctggcgaaag ggggatgtgc 2760 tgcaaggcga ttaagttggg
taacgccagg gttttcccag tcacgacgtt gtaaaacgac 2820 ggccagtgaa
ttgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga 2880
ggtcgatggt gtcgataagc ttgatatcga attcatgtca cacaaaccga tcttcgcctc
2940 aaggaaacct aattctacat ccgagagact gccgagatcc agtctacact
gattaatttt 3000 cgggccaata atttaaaaaa atcgtgttat ataatattat
atgtattata tatatacatc 3060 atgatgatac tgacagtcat gtcccattgc
taaatagaca gactccatct gccgcctcca 3120 actgatgttc tcaatattta
aggggtcatc tcgcattgtt taataataaa cagactccat 3180 ctaccgcctc
caaatgatgt tctcaaaata tattgtatga acttattttt attacttagt 3240
attattagac aacttacttg ctttatgaaa aacacttcct atttaggaaa caatttataa
3300 tggcagttcg ttcatttaac aatttatgta gaataaatgt tataaatgcg
tatgggaaat 3360 cttaaatatg gatagcataa atgatatctg cattgcctaa
ttcgaaatca acagcaacga 3420 aaaaaatccc ttgtacaaca taaatagtca
tcgagaaata tcaactatca aagaacagct 3480 attcacacgt tactattgag
attattattg gacgagaatc acacactcaa ctgtctttct 3540 ctcttctaga
aatacaggta caagtatgta ctattctcat tgttcatact tctagtcatt 3600
tcatcccaca tattccttgg atttctctcc aatgaatgac attctatctt gcaaattcaa
3660 caattataat aagatatacc aaagtagcgg tatagtggca atcaaaaagc
ttctctggtg 3720 tgcttctcgt atttattttt attctaatga tccattaaag
gtatatattt atttcttgtt 3780 atataatcct tttgtttatt acatgggctg
gatacataaa ggtattttga tttaattttt 3840 tgcttaaatt caatcccccc
tcgttcagtg tcaactgtaa tggtaggaaa ttaccatact 3900 tttgaagaag
caaaaaaaat gaaagaaaaa aaaaatcgta tttccaggtt agacgttccg 3960
cagaatctag aatgcggtat gcggtacatt gttcttcgaa cgtaaaagtt gcgctccctg
4020 agatattgta catttttgct tttacaagta caagtacatc gtacaactat
gtactactgt 4080 tgatgcatcc acaacagttt gttttgtttt tttttgtttt
ttttttttct aatgattcat 4140 taccgctatg tatacctact tgtacttgta
gtaagccggg ttattggcgt tcaattaatc 4200 atagacttat gaatctgcac
ggtgtgcgct gcgagttact tttagcttat gcatgctact 4260 tgggtgtaat
attgggatct gttcggaaat caacggatgc tcaaccgatt tcgacagtaa 4320
taatttgaat cgaatcggag cctaaaatga acccgagtat atctcataaa attctcggtg
4380 agaggtctgt gactgtcagt acaaggtgcc ttcattatgc cctcaacctt
accatacctc 4440 actgaatgta gtgtacctct aaaaatgaaa tacagtgcca
aaagccaagg cactgagctc 4500 gtctaacgga cttgatatac aaccaattaa
aacaaatgaa aagaaataca gttctttgta 4560 tcatttgtaa caattaccct
gtacaaacta aggtattgaa atcccacaat attcccaaag 4620 tccacccctt
tccaaattgt catgcctaca actcatatac caagcactaa cctaccaaac 4680
accactaaaa ccccacaaaa tatatcttac cgaatataca gtaacaagct accaccacac
4740 tcgttgggtg cagtcgccag cttaaagata tctatccaca tcagccacaa
ctcccttcct 4800 ttaataaacc gactacaccc ttggctattg aggttatgag
tgaatatact gtagacaaga 4860 cactttcaag aagactgttt ccaaaacgta
ccactgtcct ccactacaaa cacacccaat 4920 ctgcttcttc tagtcaaggt
tgctacaccg gtaaattata aatcatcatt tcattagcag 4980 ggcagggccc
tttttataga gtcttataca ctagcggacc ctgccggtag accaacccgc 5040
aggcgcgtca gtttgctcct tccatcaatg cgtcgtagaa acgacttact ccttcttgag
5100 cagctccttg accttgttgg caacaagtct ccgacctcgg aggtggagga
agagcctccg 5160 atatcggcgg tagtgatacc agcctcgacg gactccttga
cggcagcctc aacagcgtca 5220 ccggcgggct tcatgttaag agagaacttg
agcatcatgg cggcagacag aatggtggca 5280 atggggttga ccttctgctt
gccgagatcg ggggcagatc cgtgacaggg ctcgtacaga 5340 ccgaacgcct
cgttggtgtc gggcagagaa gccagagagg cggagggcag cagacccaga 5400
gaaccgggga tgacggaggc ctcgtcggag atgatatcgc caaacatgtt ggtggtgatg
5460 atgataccat tcatcttgga gggctgcttg atgaggatca tggcggccga
gtcgatcagc 5520 tggtggttga gctcgagctg ggggaattcg tccttgagga
ctcgagtgac agtctttcgc 5580 caaagtcgag aggaggccag cacgttggcc
ttgtcaagag accacacggg aagagggggg 5640 ttgtgctgaa gggccaggaa
ggcggccatt cgggcaattc gctcaacctc aggaacggag 5700 taggtctcgg
tgtcggaagc gacgccagat ccgtcatcct cctttcgctc tccaaagtag 5760
atacctccga cgagctctcg gacaatgatg aagtcggtgc cctcaacgtt tcggatgggg
5820 gagagatcgg cgagcttggg cgacagcagc tggcagggtc gcaggttggc
gtacaggttc 5880 aggtcctttc gcagcttgag gagaccctgc tcgggtcgca
cgtcggttcg tccgtcggga 5940 gtggtccata cggtgttggc agcgcctccg
acagcaccga gcataataga gtcagccttt 6000 cggcagatgt cgagagtagc
gtcggtgatg ggctcgccct ccttctcaat ggcagctcct 6060 ccaatgagtc
ggtcctcaaa cacaaactcg gtgccggagg cctcagcaac agacttgagc 6120
accttgacgg cctcggcaat cacctcgggg ccacagaagt cgccgccgag aagaacaatc
6180 ttcttggagt cagtcttggt cttcttagtt tcgggttcca ttgtggatgt
gtgtggttgt 6240 atgtgtgatg tggtgtgtgg agtgaaaatc tgtggctggc
aaacgctctt gtatatatac 6300 gcacttttgc ccgtgctatg tggaagacta
aacctccgaa gattgtgact caggtagtgc 6360 ggtatcggct agggacccaa
accttgtcga tgccgatagc gctatcgaac gtaccccagc 6420 cggccgggag
tatgtcggag gggacatacg agatcgtcaa gggtttgtgg ccaactggta 6480
aataaatgat gtcgacgttt aaacagtgta cgcagtacta tagaggaaca attgccccgg
6540 agaagacggc caggccgcct agatgacaaa ttcaacaact cacagctgac
tttctgccat 6600
tgccactagg ggggggcctt tttatatggc caagccaagc tctccacgtc ggttgggctg
6660 cacccaacaa taaatgggta gggttgcacc aacaaaggga tgggatgggg
ggtagaagat 6720 acgaggataa cggggctcaa tggcacaaat aagaacgaat
actgccatta agactcgtga 6780 tccagcgact gacaccattg catcatctaa
gggcctcaaa actacctcgg aactgctgcg 6840 ctgatctgga caccacagag
gttccgagca ctttaggttg caccaaatgt cccaccaggt 6900 gcaggcagaa
aacgctggaa cagcgtgtac agtttgtctt aacaaaaagt gagggcgctg 6960
aggtcgagca gggtggtgtg acttgttata gcctttagag ctgcgaaagc gcgtatggat
7020 ttggctcatc aggccagatt gagggtctgt ggacacatgt catgttagtg
tacttcaatc 7080 gccccctgga tatagccccg acaataggcc gtggcctcat
ttttttgcct tccgcacatt 7140 tccattgctc ggtacccaca ccttgcttct
cctgcacttg ccaaccttaa tactggttta 7200 cattgaccaa catcttacaa
gcggggggct tgtctagggt atatataaac agtggctctc 7260 ccaatcggtt
gccagtctct tttttccttt ctttccccac agattcgaaa tctaaactac 7320
acatcacaca atgcctgtta ctgacgtcct taagcgaaag tccggtgtca tcgtcggcga
7380 cgatgtccga gccgtgagta tccacgacaa gatcagtgtc gagacgacgc
gttttgtgta 7440 atgacacaat ccgaaagtcg ctagcaacac acactctcta
cacaaactaa cccagctctc 7500 catggcatgg atggtacgtc ctgtagaaac
cccaacccgt gaaatcaaaa aactcgacgg 7560 cctgtgggca ttcagtctgg
atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag 7620 cgcgttacaa
gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga 7680
tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
7740 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
acggcaaagt 7800 gtgggtcaat aatcaggaag tgatggagca tcagggcggc
tatacgccat ttgaagccga 7860 tgtcacgccg tatgttattg ccgggaaaag
tgtacgtatc accgtttgtg tgaacaacga 7920 actgaactgg cagactatcc
cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa 7980 gcagtcttac
ttccatgatt tctttaacta tgccgggatc catcgcagcg taatgctcta 8040
caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
8100 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
ttgaactgcg 8160 tgatgcggat caacaggtgg ttgcaactgg acaaggcact
agcgggactt tgcaagtggt 8220 gaatccgcac ctctggcaac cgggtgaagg
ttatctctat gaactgtgcg tcacagccaa 8280 aagccagaca gagtgtgata
tctacccgct tcgcgtcggc atccggtcag tggcagtgaa 8340 gggcgaacag
ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga 8400
agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
8460 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
ctgaagagat 8520 gctcgactgg gcagatgaac atggcatcgt ggtgattgat
gaaactgctg ctgtcggctt 8580 taacctctct ttaggcattg gtttcgaagc
gggcaacaag ccgaaagaac tgtacagcga 8640 agaggcagtc aacggggaaa
ctcagcaagc gcacttacag gcgattaaag agctgatagc 8700 gcgtgacaaa
aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg 8760
tccgcaagtg cacgggaata tttcgccact ggcggaagca acgcgtaaac tcgacccgac
8820 gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata
ccatcagcga 8880 tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
tatgtccaaa gcggcgattt 8940 ggaaacggca gagaaggtac tggaaaaaga
acttctggcc tggcaggaga aactgcatca 9000 gccgattatc atcaccgaat
acggcgtgga tacgttagcc gggctgcact caatgtacac 9060 cgacatgtgg
agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga 9120
tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca
9180 aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc
gcaaaccgaa 9240 gtcggcggct tttctgctgc aaaaacgctg gactggcatg
aacttcggtg aaaaaccgca 9300 gcagggaggc aaacaatgat taattaacta
gagcggccgc caccgcggcc cgagattccg 9360 gcctcttcgg ccgccaagcg
acccgggtgg acgtctagag gtacctagca attaacagat 9420 agtttgccgg
tgataattct cttaacctcc cacactcctt tgacataacg atttatgtaa 9480
cgaaactgaa atttgaccag atattgtgtc cgc 9513 <210> SEQ ID NO 51
<211> LENGTH: 8910 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pFmD8S <400> SEQUENCE: 51
catggtgaag tccaagcgac aggctctgcc cctcaccatc gacggaacta cctacgacgt
60 ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact
accagggacg 120 agatgctact gacgccttca tggttatgca ctctcaggaa
gccttcgaca agctcaagcg 180 aatgcccaag atcaacccct cctccgagct
gcctccccag gctgccgtca acgaagctca 240 ggaggatttc cgaaagctcc
gagaagagct gatcgccact ggcatgtttg acgcctctcc 300 cctctggtac
tcgtacaaga tctccaccac cctgggtctt ggcgtgcttg gatacttcct 360
gatggtccag taccagatgt acttcattgg tgctgtgctg ctcggtatgc actaccagca
420 aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc
gaaactggaa 480 taacctcgtg ggtctggtct ttggcaacgg actccagggc
ttctccgtga cctggtggaa 540 ggacagacac aacgcccatc attctgctac
caacgttcag ggtcacgatc ccgacattga 600 taacctgcct ctgctcgcct
ggtccgagga cgatgtcact cgagcttctc ccatctcccg 660 aaagctcatt
cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat 720
ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta
780 ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc
tcaagaccct 840 gttccacctc ttctttatgc cctccatcct gacctcgctc
ctggtgttct ttgtttccga 900 gctcgtcggt ggcttcggaa ttgccatcgt
ggtcttcatg aaccactacc ctctggagaa 960 gatcggtgat tccgtctggg
acggacatgg cttctctgtg ggtcagatcc atgagaccat 1020 gaacattcga
cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga 1080
gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga
1140 acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg
aaggtctcgt 1200 catcctgctc cgatacctgg ccgtgttcgc tcgaatggcc
gagaagcagc ccgctggcaa 1260 ggctctctaa gcggccgcca ccgcggcccg
agattccggc ctcttcggcc gccaagcgac 1320 ccgggtggac gtctagaggt
acctagcaat taacagatag tttgccggtg ataattctct 1380 taacctccca
cactcctttg acataacgat ttatgtaacg aaactgaaat ttgaccagat 1440
attgtgtccg cggtggagct ccagcttttg ttccctttag tgagggttaa tttcgagctt
1500 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
caattccaca 1560 caacatacga gccggaagca taaagtgtaa agcctggggt
gcctaatgag tgagctaact 1620 cacattaatt gcgttgcgct cactgcccgc
tttccagtcg ggaaacctgt cgtgccagct 1680 gcattaatga atcggccaac
gcgcggggag aggcggtttg cgtattgggc gctcttccgc 1740 ttcctcgctc
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1800
ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1860 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
cgtttttcca 1920 taggctccgc ccccctgacg agcatcacaa aaatcgacgc
tcaagtcaga ggtggcgaaa 1980 cccgacagga ctataaagat accaggcgtt
tccccctgga agctccctcg tgcgctctcc 2040 tgttccgacc ctgccgctta
ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2100 gctttctcat
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2160
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2220 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
ctggtaacag 2280 gattagcaga gcgaggtatg taggcggtgc tacagagttc
ttgaagtggt ggcctaacta 2340 cggctacact agaaggacag tatttggtat
ctgcgctctg ctgaagccag ttaccttcgg 2400 aaaaagagtt ggtagctctt
gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2460 tgtttgcaag
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 2520
ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2580 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
ttaaatcaat 2640 ctaaagtata tatgagtaaa cttggtctga cagttaccaa
tgcttaatca gtgaggcacc 2700 tatctcagcg atctgtctat ttcgttcatc
catagttgcc tgactccccg tcgtgtagat 2760 aactacgata cgggagggct
taccatctgg ccccagtgct gcaatgatac cgcgagaccc 2820 acgctcaccg
gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 2880
aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2940 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
caggcatcgt 3000 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
ggttcccaac gatcaaggcg 3060 agttacatga tcccccatgt tgtgcaaaaa
agcggttagc tccttcggtc ctccgatcgt 3120 tgtcagaagt aagttggccg
cagtgttatc actcatggtt atggcagcac tgcataattc 3180 tcttactgtc
atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3240
attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
3300 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
cttcggggcg 3360 aaaactctca aggatcttac cgctgttgag atccagttcg
atgtaaccca ctcgtgcacc 3420 caactgatct tcagcatctt ttactttcac
cagcgtttct gggtgagcaa aaacaggaag 3480 gcaaaatgcc gcaaaaaagg
gaataagggc gacacggaaa tgttgaatac tcatactctt 3540 cctttttcaa
tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3600
tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3660 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
cgcgcagcgt 3720 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
gctttcttcc cttcctttct 3780 cgccacgttc gccggctttc cccgtcaagc
tctaaatcgg gggctccctt tagggttccg 3840 atttagtgct ttacggcacc
tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3900 tgggccatcg
ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3960
tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
4020 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
tttaacaaaa 4080 atttaacgcg aattttaaca aaatattaac gcttacaatt
tccattcgcc attcaggctg 4140 cgcaactgtt gggaagggcg atcggtgcgg
gcctcttcgc tattacgcca gctggcgaaa 4200 gggggatgtg ctgcaaggcg
attaagttgg gtaacgccag ggttttccca gtcacgacgt 4260 tgtaaaacga
cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg 4320
ccccccctcg aggtcgatgg tgtcgataag cttgatatcg aattcatgtc acacaaaccg
4380 atcttcgcct caaggaaacc taattctaca tccgagagac tgccgagatc
cagtctacac 4440 tgattaattt tcgggccaat aatttaaaaa aatcgtgtta
tataatatta tatgtattat 4500 atatatacat catgatgata ctgacagtca
tgtcccattg ctaaatagac agactccatc 4560 tgccgcctcc aactgatgtt
ctcaatattt aaggggtcat ctcgcattgt ttaataataa 4620 acagactcca
tctaccgcct ccaaatgatg ttctcaaaat atattgtatg aacttatttt 4680
tattacttag tattattaga caacttactt gctttatgaa aaacacttcc tatttaggaa
4740 acaatttata atggcagttc gttcatttaa caatttatgt agaataaatg
ttataaatgc 4800 gtatgggaaa tcttaaatat ggatagcata aatgatatct
gcattgccta attcgaaatc 4860 aacagcaacg aaaaaaatcc cttgtacaac
ataaatagtc atcgagaaat atcaactatc 4920 aaagaacagc tattcacacg
ttactattga gattattatt ggacgagaat cacacactca 4980 actgtctttc
tctcttctag aaatacaggt acaagtatgt actattctca ttgttcatac 5040
ttctagtcat ttcatcccac atattccttg gatttctctc caatgaatga cattctatct
5100 tgcaaattca acaattataa taagatatac caaagtagcg gtatagtggc
aatcaaaaag 5160 cttctctggt gtgcttctcg tatttatttt tattctaatg
atccattaaa ggtatatatt 5220 tatttcttgt tatataatcc ttttgtttat
tacatgggct ggatacataa aggtattttg 5280 atttaatttt ttgcttaaat
tcaatccccc ctcgttcagt gtcaactgta atggtaggaa 5340 attaccatac
ttttgaagaa gcaaaaaaaa tgaaagaaaa aaaaaatcgt atttccaggt 5400
tagacgttcc gcagaatcta gaatgcggta tgcggtacat tgttcttcga acgtaaaagt
5460 tgcgctccct gagatattgt acatttttgc ttttacaagt acaagtacat
cgtacaacta 5520 tgtactactg ttgatgcatc cacaacagtt tgttttgttt
ttttttgttt tttttttttc 5580 taatgattca ttaccgctat gtatacctac
ttgtacttgt agtaagccgg gttattggcg 5640 ttcaattaat catagactta
tgaatctgca cggtgtgcgc tgcgagttac ttttagctta 5700 tgcatgctac
ttgggtgtaa tattgggatc tgttcggaaa tcaacggatg ctcaaccgat 5760
ttcgacagta ataatttgaa tcgaatcgga gcctaaaatg aacccgagta tatctcataa
5820 aattctcggt gagaggtctg tgactgtcag tacaaggtgc cttcattatg
ccctcaacct 5880 taccatacct cactgaatgt agtgtacctc taaaaatgaa
atacagtgcc aaaagccaag 5940 gcactgagct cgtctaacgg acttgatata
caaccaatta aaacaaatga aaagaaatac 6000 agttctttgt atcatttgta
acaattaccc tgtacaaact aaggtattga aatcccacaa 6060 tattcccaaa
gtccacccct ttccaaattg tcatgcctac aactcatata ccaagcacta 6120
acctaccaaa caccactaaa accccacaaa atatatctta ccgaatatac agtaacaagc
6180 taccaccaca ctcgttgggt gcagtcgcca gcttaaagat atctatccac
atcagccaca 6240 actcccttcc tttaataaac cgactacacc cttggctatt
gaggttatga gtgaatatac 6300 tgtagacaag acactttcaa gaagactgtt
tccaaaacgt accactgtcc tccactacaa 6360 acacacccaa tctgcttctt
ctagtcaagg ttgctacacc ggtaaattat aaatcatcat 6420 ttcattagca
gggcagggcc ctttttatag agtcttatac actagcggac cctgccggta 6480
gaccaacccg caggcgcgtc agtttgctcc ttccatcaat gcgtcgtaga aacgacttac
6540 tccttcttga gcagctcctt gaccttgttg gcaacaagtc tccgacctcg
gaggtggagg 6600 aagagcctcc gatatcggcg gtagtgatac cagcctcgac
ggactccttg acggcagcct 6660 caacagcgtc accggcgggc ttcatgttaa
gagagaactt gagcatcatg gcggcagaca 6720 gaatggtggc aatggggttg
accttctgct tgccgagatc gggggcagat ccgtgacagg 6780 gctcgtacag
accgaacgcc tcgttggtgt cgggcagaga agccagagag gcggagggca 6840
gcagacccag agaaccgggg atgacggagg cctcgtcgga gatgatatcg ccaaacatgt
6900 tggtggtgat gatgatacca ttcatcttgg agggctgctt gatgaggatc
atggcggccg 6960 agtcgatcag ctggtggttg agctcgagct gggggaattc
gtccttgagg actcgagtga 7020 cagtctttcg ccaaagtcga gaggaggcca
gcacgttggc cttgtcaaga gaccacacgg 7080 gaagaggggg gttgtgctga
agggccagga aggcggccat tcgggcaatt cgctcaacct 7140 caggaacgga
gtaggtctcg gtgtcggaag cgacgccaga tccgtcatcc tcctttcgct 7200
ctccaaagta gatacctccg acgagctctc ggacaatgat gaagtcggtg ccctcaacgt
7260 ttcggatggg ggagagatcg gcgagcttgg gcgacagcag ctggcagggt
cgcaggttgg 7320 cgtacaggtt caggtccttt cgcagcttga ggagaccctg
ctcgggtcgc acgtcggttc 7380 gtccgtcggg agtggtccat acggtgttgg
cagcgcctcc gacagcaccg agcataatag 7440 agtcagcctt tcggcagatg
tcgagagtag cgtcggtgat gggctcgccc tccttctcaa 7500 tggcagctcc
tccaatgagt cggtcctcaa acacaaactc ggtgccggag gcctcagcaa 7560
cagacttgag caccttgacg gcctcggcaa tcacctcggg gccacagaag tcgccgccga
7620 gaagaacaat cttcttggag tcagtcttgg tcttcttagt ttcgggttcc
attgtggatg 7680 tgtgtggttg tatgtgtgat gtggtgtgtg gagtgaaaat
ctgtggctgg caaacgctct 7740 tgtatatata cgcacttttg cccgtgctat
gtggaagact aaacctccga agattgtgac 7800 tcaggtagtg cggtatcggc
tagggaccca aaccttgtcg atgccgatag cgctatcgaa 7860 cgtaccccag
ccggccggga gtatgtcgga ggggacatac gagatcgtca agggtttgtg 7920
gccaactggt aaataaatga tgtcgacgtt taaacagtgt acgcagatct actatagagg
7980 aacatttaaa ttgccccgga gaagacggcc aggccgccta gatgacaaat
tcaacaactc 8040 acagctgact ttctgccatt gccactaggg gggggccttt
ttatatggcc aagccaagct 8100 ctccacgtcg gttgggctgc acccaacaat
aaatgggtag ggttgcacca acaaagggat 8160 gggatggggg gtagaagata
cgaggataac ggggctcaat ggcacaaata agaacgaata 8220 ctgccattaa
gactcgtgat ccagcgactg acaccattgc atcatctaag ggcctcaaaa 8280
ctacctcgga actgctgcgc tgatctggac accacagagg ttccgagcac tttaggttgc
8340 accaaatgtc ccaccaggtg caggcagaaa acgctggaac agcgtgtaca
gtttgtctta 8400 acaaaaagtg agggcgctga ggtcgagcag ggtggtgtga
cttgttatag cctttagagc 8460 tgcgaaagcg cgtatggatt tggctcatca
ggccagattg agggtctgtg gacacatgtc 8520 atgttagtgt acttcaatcg
ccccctggat atagccccga caataggccg tggcctcatt 8580 tttttgcctt
ccgcacattt ccattgctcg gtacccacac cttgcttctc ctgcacttgc 8640
caaccttaat actggtttac attgaccaac atcttacaag cggggggctt gtctagggta
8700 tatataaaca gtggctctcc caatcggttg ccagtctctt ttttcctttc
tttccccaca 8760 gattcgaaat ctaaactaca catcacagaa ttccgagccg
tgagtatcca cgacaagatc 8820 agtgtcgaga cgacgcgttt tgtgtaatga
cacaatccga aagtcgctag caacacacac 8880 tctctacaca aactaaccca
gctctggtac 8910 <210> SEQ ID NO 52 <211> LENGTH: 1272
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(1270) <223> OTHER INFORMATION: synthetic
delta-8 desaturase CDS, codon-optimized for Yarrowia lipolytica
("D8SF" or "EgD8S") <300> PUBLICATION INFORMATION:
<302> TITLE: DELTA-8 DESATURASE AND ITS USE IN MAKING
POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT NUMBER: WO
2006/012325 and WO 2006/012326 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2006-02-02 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(1272) <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-8 DESATURASE AND ITS USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: US-2005-0287652-A1 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2005-12-29 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(1272) <400> SEQUENCE:
52 catggtgaag tccaagcgac aggctctgcc cctcaccatc gacggaacta
cctacgacgt 60 ctccgcttgg gtgaacttcc accctggtgg agctgaaatc
attgagaact accagggacg 120 agatgctact gacgccttca tggttatgca
ctctcaggaa gccttcgaca agctcaagcg 180 aatgcccaag atcaacccct
cctccgagct gcctccccag gctgccgtca acgaagctca 240 ggaggatttc
cgaaagctcc gagaagagct gatcgccact ggcatgtttg acgcctctcc 300
cctctggtac tcgtacaaga tctccaccac cctgggtctt ggcgtgcttg gatacttcct
360 gatggtccag taccagatgt acttcattgg tgctgtgctg ctcggtatgc
actaccagca 420 aatgggatgg ctgtctcatg acatctgcca ccaccagacc
ttcaagaacc gaaactggaa 480 taacctcgtg ggtctggtct ttggcaacgg
actccagggc ttctccgtga cctggtggaa 540 ggacagacac aacgcccatc
attctgctac caacgttcag ggtcacgatc ccgacattga 600 taacctgcct
ctgctcgcct ggtccgagga cgatgtcact cgagcttctc ccatctcccg 660
aaagctcatt cagttccaac agtactattt cctggtcatc tgtattctcc tgcgattcat
720 ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag gaccgagaca
accagttcta 780 ccgatctcag tacaagaaag aggccattgg actcgctctg
cactggactc tcaagaccct 840 gttccacctc ttctttatgc cctccatcct
gacctcgctc ctggtgttct ttgtttccga 900 gctcgtcggt ggcttcggaa
ttgccatcgt ggtcttcatg aaccactacc ctctggagaa 960 gatcggtgat
tccgtctggg acggacatgg cttctctgtg ggtcagatcc atgagaccat 1020
gaacattcga cgaggcatca ttactgactg gttctttgga ggcctgaact accagatcga
1080 gcaccatctc tggcccaccc tgcctcgaca caacctcact gccgtttcct
accaggtgga 1140 acagctgtgc cagaagcaca acctccccta ccgaaaccct
ctgccccatg aaggtctcgt 1200 catcctgctc cgatacctgg ccgtgttcgc
tcgaatggcc gagaagcagc ccgctggcaa 1260 ggctctctaa gc 1272
<210> SEQ ID NO 53 <211> LENGTH: 422 <212> TYPE:
PRT <213> ORGANISM: Euglena gracilis <220> FEATURE:
<221> NAME/KEY: MISC_FEATURE <223> OTHER INFORMATION:
synthetic delta-8 desaturase codon-optimized for Yarrowia
lipolytica ("D8SF" or "EgD8S") <300> PUBLICATION INFORMATION:
<302> TITLE: DELTA-8 DESATURASE AND ITS USE IN MAKING
POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT NUMBER: WO
2006/012325 and WO 2006/012326 <311> PATENT FILING DATE:
2005-06-24 <312> PUBLICATION DATE: 2006-02-02 <313>
RELEVANT RESIDUES IN SEQ ID NO: (1)..(422) <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-8 DESATURASE AND ITS USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: US-2005-0287652-A1
<311> PATENT FILING DATE: 2005-06-24 <312> PUBLICATION
DATE: 2005-12-29 <313> RELEVANT RESIDUES IN SEQ ID NO:
(1)..(422) <400> SEQUENCE: 53 Met Val Lys Ser Lys Arg Gln Ala
Leu Pro Leu Thr Ile Asp Gly Thr 1 5 10 15 Thr Tyr Asp Val Ser Ala
Trp Val Asn Phe His Pro Gly Gly Ala Glu 20 25 30 Ile Ile Glu Asn
Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 35 40 45 Met His
Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 50 55 60
Asn Pro Ser Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 65
70 75 80 Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly
Met Phe 85 90 95 Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Ser
Thr Thr Leu Gly 100 105 110 Leu Gly Val Leu Gly Tyr Phe Leu Met Val
Gln Tyr Gln Met Tyr Phe 115 120 125 Ile Gly Ala Val Leu Leu Gly Met
His Tyr Gln Gln Met Gly Trp Leu 130 135 140 Ser His Asp Ile Cys His
His Gln Thr Phe Lys Asn Arg Asn Trp Asn 145 150 155 160 Asn Leu Val
Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 165 170 175 Thr
Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 180 185
190 Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser
195 200 205 Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu
Ile Gln 210 215 220 Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu
Leu Arg Phe Ile 225 230 235 240 Trp Cys Phe Gln Ser Val Leu Thr Val
Arg Ser Leu Lys Asp Arg Asp 245 250 255 Asn Gln Phe Tyr Arg Ser Gln
Tyr Lys Lys Glu Ala Ile Gly Leu Ala 260 265 270 Leu His Trp Thr Leu
Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 275 280 285 Ile Leu Thr
Ser Leu Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 290 295 300 Phe
Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 305 310
315 320 Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln
Ile 325 330 335 His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp
Trp Phe Phe 340 345 350 Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu
Trp Pro Thr Leu Pro 355 360 365 Arg His Asn Leu Thr Ala Val Ser Tyr
Gln Val Glu Gln Leu Cys Gln 370 375 380 Lys His Asn Leu Pro Tyr Arg
Asn Pro Leu Pro His Glu Gly Leu Val 385 390 395 400 Ile Leu Leu Arg
Tyr Leu Ala Val Phe Ala Arg Met Ala Glu Lys Gln 405 410 415 Pro Ala
Gly Lys Ala Leu 420 <210> SEQ ID NO 54 <211> LENGTH: 27
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Primer PUD17-F
<400> SEQUENCE: 54 aagatcccat ggcttcttcc actgttg 27
<210> SEQ ID NO 55 <211> LENGTH: 33 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Primer PUD17-R <400> SEQUENCE:
55 atcatcgcgg ccgcctagtt ggccttggtc ttg 33 <210> SEQ ID NO 56
<211> LENGTH: 12649 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pKUNF12T6E <220> FEATURE:
<221> NAME/KEY: misc_feature <222> LOCATION:
(2507)..(2507) <223> OTHER INFORMATION: n is a, c, g, or t
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2512)..(2515) <223> OTHER INFORMATION: n is a, c,
g, or t <400> SEQUENCE: 56 taaccctcac taaagggaac aaaagctgga
gctccaccgc ggacacaata tctggtcaaa 60 tttcagtttc gttacataaa
tcgttatgtc aaaggagtgt gggaggttaa gagaattatc 120 accggcaaac
tatctgttaa ttgctaggta cctctagacg tccacccggg tcgcttggcg 180
gccgaagagg ccggaatctc gggccgcggt ggcggccgct tagttggtct tggacttctt
240 gggcttcttc aggtaggact ggacaaagaa gttgccgaac agagcgagca
gggtgatcat 300 gtacacgccg agcagctgga ccagagcctg agggtagtcg
caggggaaga ggtagtcgta 360 cagggactgc accagcatag ccatgaactg
ggtcatctgc agagtggtga tgtagggctt 420 gatgggcttg acgaagccga
agccctgaga ggaaaagaag tagtaggcgt acatgacggt 480 gtggacgaag
gagttgagga tgacggagaa gtaggcgtcg ccaccaggag cgtacttggc 540
aatagcccac cagatggcga agatggtggc atggtggtac acgtgcagga aggagacctg
600 gttgaacttc ttgcacagga tcatgatagc ggtgtccagg aactcgtagg
ccttggagac 660 gtagaacacg tagacgattc gggacatgcc ctgagcgtgg
gactcgttgc ccttctccat 720 gtcgttgccg aagaccttgt agccacccag
gatagcctgt cggatggtct cgacgcacat 780 gtagagggac agtccgaaga
ggaacaggtt gtggagcagc ttgatggtct tcagctcgaa 840 gggcttctcc
atctgcttca tgatgggaat gccgaagagc agcatggcca tgtagccgac 900
ctcgaaggcg agcatggtgg agacgtccat catgggcaga ccgtcggtca gagcgtaggg
960 cttagctccg tccatccact ggtcgacacc ggtctcgact cgtccgacca
cgtcgtccca 1020 gacagaggag ttggccatgg tgaatgattc ttatactcag
aaggaaatgc ttaacgattt 1080 cgggtgtgag ttgacaagga gagagagaaa
agaagaggaa aggtaattcg gggacggtgg 1140 tcttttatac ccttggctaa
agtcccaacc acaaagcaaa aaaattttca gtagtctatt 1200 ttgcgtccgg
catgggttac ccggatggcc agacaaagaa actagtacaa agtctgaaca 1260
agcgtagatt ccagactgca gtaccctacg cccttaacgg caagtgtggg aaccggggga
1320 ggtttgatat gtggggtgaa gggggctctc gccggggttg ggcccgctac
tgggtcaatt 1380 tggggtcaat tggggcaatt ggggctgttt tttgggacac
aaatacgccg ccaacccggt 1440 ctctcctgaa ttctgcatcg atcgaggaag
aggacaagcg gctgcttctt aagtttgtga 1500 catcagtatc caaggcacca
ttgcaaggat tcaaggcttt gaacccgtca tttgccattc 1560 gtaacgctgg
tagacaggtt gatcggttcc ctacggcctc cacctgtgtc aatcttctca 1620
agctgcctga ctatcaggac attgatcaac ttcggaagaa acttttgtat gccattcgat
1680 cacatgctgg tttcgatttg tcttagagga acgcatatac agtaatcata
gagaataaac 1740 gatattcatt tattaaagta gatagttgag gtagaagttg
taaagagtga taaatagcgg 1800 ccgcgcctac ttaagcaacg ggcttgataa
cagcgggggg ggtgcccacg ttgttgcggt 1860 tgcggaagaa cagaacaccc
ttaccagcac cctcggcacc agcgctgggc tcaacccact 1920 ggcacatacg
cgcactgcgg tacatggcgc ggatgaagcc acgaggacca tcctggacat 1980
cagcccggta gtgcttgccc atgatgggct taatggcctc ggtggcctcg tccgcgttgt
2040 agaaggggat gctgctgacg tagtggtgga ggacatgagt ctcgatgatg
ccgtggagaa 2100 ggtggcggcc gatgaagccc atctcacggt caatggtagc
agcggcacca cggacgaagt 2160 tccactcgtc gttggtgtag tggggaaggg
tagggtcggt gtgctggagg aaggtgatgg 2220 caacgagcca gtggttaacc
cagaggtagg gaacaaagta ccagatggcc atgttgtaga 2280 aaccgaactt
ctgaacgagg aagtacagag cagtggccat cagaccgata ccaatatcgc 2340
tgaggacgat gagcttagcg tcactgttct cgtacagagg gctgcgggga tcgaagtggt
2400 taacaccacc gccgaggccg ttatgcttgc ccttgccgcg accctcacgc
tggcgctcgt 2460 ggtagttgtg gccggtaaca ttggtgatga ggtagttggg
ccagccnacg annnnctcag 2520 taagatgagc gagctcgtgg gtcatctttc
cgagacgagt agcctgctgc tcgcgggttc 2580 ggggaacgaa gaccatgtca
cgctccatgt tgccagtggc cttgtggtgc tttcggtggg 2640 agatttgcca
gctgaagtag gggacaagga gggaagagtg aagaacccag ccagtaatgt 2700
cgttgatgat gcgagaatcg gagaaagcac cgtgaccgca ctcatgggca ataacccaga
2760 gaccagtacc gaaaagaccc tgaagaacgg tgtacacggc ccacagacca
gcgcgggcgg 2820 gggtggaggg gatatattcg ggggtcacaa agttgtacca
gatgctgaaa gtggtagtca 2880 ggaggacaat gtcgcggagg atataaccgt
atcccttgag agcggagcgc ttgaagcagt 2940 gcttagggat ggcattgtag
atgtccttga tggtaaagtc gggaacctcg aactggttgc 3000 cgtaggtgtc
gagcatgaca ccatactcgg acttgggctt ggcgatatca acctcggaca 3060
tggacgagag cgatgtggaa gaggccgagt ggcggggaga gtctgaagga gagacggcgg
3120 cagactcaga atccgtcaca gtagttgagg tgacggtgcg tctaagcgca
gggttctgct 3180 tgggcagagc cgaagtggac gccatggaga gctgggttag
tttgtgtaga gagtgtgtgt 3240 tgctagcgac tttcggattg tgtcattaca
caaaacgcgt cgtctcgaca ctgatcttgt 3300 cgtggatact cacggctcgg
acatcgtcgc cgacgatgac accggacttt cgcttaagga 3360 cgtcagtaac
aggcattgtg tgatgtgtag tttagatttc gaatctgtgg ggaaagaaag 3420
gaaaaaagag actggcaacc gattgggaga gccactgttt atatataccc tagacaagcc
3480 ccccgcttgt aagatgttgg tcaatgtaaa ccagtattaa ggttggcaag
tgcaggagaa 3540 gcaaggtgtg ggtaccgagc aatggaaatg tgcggaaggc
aaaaaaatga ggccacggcc 3600 tattgtcggg gctatatcca gggggcgatt
gaagtacact aacatgacat gtgtccacag 3660
accctcaatc tggcctgatg agccaaatcc atacgcgctt tcgcagctct aaaggctata
3720 acaagtcaca ccaccctgct cgacctcagc gccctcactt tttgttaaga
caaactgtac 3780 acgctgttcc agcgttttct gcctgcacct ggtgggacat
ttggtgcaac ctaaagtgct 3840 cggaacctct gtggtgtcca gatcagcgca
gcagttccga ggtagttttg aggcccttag 3900 atgatgcaat ggtgtcagtc
gctggatcac gagtcttaat ggcagtattc gttcttattt 3960 gtgccattga
gccccgttat cctcgtatct tctacccccc atcccatccc tttgttggtg 4020
caaccctacc catttattgt tgggtgcagc ccaaccgacg tggagagctt ggcttggcca
4080 tataaaaagg ccccccccta gtggcaatgg cagaaagtca gctgtgagtt
gttgaatttg 4140 tcatctaggc ggcctggccg tcttctccgg ggcaattgtt
cctctatagt actgcgtaca 4200 ctgtttaaac agtgtacgca gatctgcgac
gacggaattc ctgcagccca tctgcagaat 4260 tcaggagaga ccgggttggc
ggcgtatttg tgtcccaaaa aacagcccca attgccccaa 4320 ttgaccccaa
attgacccag tagcgggccc aaccccggcg agagccccct tcaccccaca 4380
tatcaaacct cccccggttc ccacacttgc cgttaagggc gtagggtact gcagtctgga
4440 atctacgctt gttcagactt tgtactagtt tctttgtctg gccatccggg
taacccatgc 4500 cggacgcaaa atagactact gaaaattttt ttgctttgtg
gttgggactt tagccaaggg 4560 tataaaagac caccgtcccc gaattacctt
tcctcttctt ttctctctct ccttgtcaac 4620 tcacacccga aatcgttaag
catttccttc tgagtataag aatcattcac catggctgcc 4680 gctccctctg
tgcgaacctt tacccgagcc gaggttctga acgctgaggc tctgaacgag 4740
ggcaagaagg acgctgaggc tcccttcctg atgatcatcg acaacaaggt gtacgacgtc
4800 cgagagttcg tccctgacca tcctggaggc tccgtgattc tcacccacgt
tggcaaggac 4860 ggcaccgacg tctttgacac ctttcatccc gaggctgctt
gggagactct cgccaacttc 4920 tacgttggag acattgacga gtccgaccga
gacatcaaga acgatgactt tgccgctgag 4980 gtccgaaagc tgcgaaccct
gttccagtct ctcggctact acgactcctc taaggcctac 5040 tacgccttca
aggtctcctt caacctctgc atctggggac tgtccaccgt cattgtggcc 5100
aagtggggtc agacctccac cctcgccaac gtgctctctg ctgccctgct cggcctgttc
5160 tggcagcagt gcggatggct ggctcacgac tttctgcacc accaggtctt
ccaggaccga 5220 ttctggggtg atctcttcgg agccttcctg ggaggtgtct
gccagggctt ctcctcttcc 5280 tggtggaagg acaagcacaa cactcaccat
gccgctccca acgtgcatgg cgaggatcct 5340 gacattgaca cccaccctct
cctgacctgg tccgagcacg ctctggagat gttctccgac 5400 gtccccgatg
aggagctgac ccgaatgtgg tctcgattca tggtcctgaa ccagacctgg 5460
ttctacttcc ccattctctc cttcgctcga ctgtcttggt gcctccagtc cattctcttt
5520 gtgctgccca acggtcaggc tcacaagccc tccggagctc gagtgcccat
ctccctggtc 5580 gagcagctgt ccctcgccat gcactggacc tggtacctcg
ctaccatgtt cctgttcatc 5640 aaggatcctg tcaacatgct cgtgtacttc
ctggtgtctc aggctgtgtg cggaaacctg 5700 ctcgccatcg tgttctccct
caaccacaac ggtatgcctg tgatctccaa ggaggaggct 5760 gtcgacatgg
atttctttac caagcagatc atcactggtc gagatgtcca tcctggactg 5820
ttcgccaact ggttcaccgg tggcctgaac taccagatcg agcatcacct gttcccttcc
5880 atgcctcgac acaacttctc caagatccag cctgccgtcg agaccctgtg
caagaagtac 5940 aacgtccgat accacaccac tggtatgatc gagggaactg
ccgaggtctt ctcccgactg 6000 aacgaggtct ccaaggccac ctccaagatg
ggcaaggctc agtaagcggc cgcatgagaa 6060 gataaatata taaatacatt
gagatattaa atgcgctaga ttagagagcc tcatactgct 6120 cggagagaag
ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa 6180
gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg
6240 gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc
ttggcctcca 6300 tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa
cagaagcaga tgaagaatga 6360 acttgaagtg aaggaattta aattgccccg
gagaagacgg ccaggccgcc tagatgacaa 6420 attcaacaac tcacagctga
ctttctgcca ttgccactag gggggggcct ttttatatgg 6480 ccaagccaag
ctctccacgt cggttgggct gcacccaaca ataaatgggt agggttgcac 6540
caacaaaggg atgggatggg gggtagaaga tacgaggata acggggctca atggcacaaa
6600 taagaacgaa tactgccatt aagactcgtg atccagcgac tgacaccatt
gcatcatcta 6660 agggcctcaa aactacctcg gaactgctgc gctgatctgg
acaccacaga ggttccgagc 6720 actttaggtt gcaccaaatg tcccaccagg
tgcaggcaga aaacgctgga acagcgtgta 6780 cagtttgtct taacaaaaag
tgagggcgct gaggtcgagc agggtggtgt gacttgttat 6840 agcctttaga
gctgcgaaag cgcgtatgga tttggctcat caggccagat tgagggtctg 6900
tggacacatg tcatgttagt gtacttcaat cgccccctgg atatagcccc gacaataggc
6960 cgtggcctca tttttttgcc ttccgcacat ttccattgct cggtacccac
accttgcttc 7020 tcctgcactt gccaacctta atactggttt acattgacca
acatcttaca agcggggggc 7080 ttgtctaggg tatatataaa cagtggctct
cccaatcggt tgccagtctc ttttttcctt 7140 tctttcccca cagattcgaa
atctaaacta cacatcacac aatgcctgtt actgacgtcc 7200 ttaagcgaaa
gtccggtgtc atcgtcggcg acgatgtccg agccgtgagt atccacgaca 7260
agatcagtgt cgagacgacg cgttttgtgt aatgacacaa tccgaaagtc gctagcaaca
7320 cacactctct acacaaacta acccagctct ccatggagtc cattgctccc
ttcctgccct 7380 ccaagatgcc tcaggacctg ttcatggacc tcgccagcgc
tatcggtgtc cgagctgctc 7440 cctacgtcga tcccctggag gctgccctgg
ttgcccaggc cgagaagtac attcccacca 7500 ttgtccatca cactcgaggc
ttcctggttg ccgtggagtc tcccctggct cgagagctgc 7560 ctctgatgaa
ccccttccac gtgctcctga tcgtgctcgc ctacctggtc accgtgtttg 7620
tgggtatgca gatcatgaag aactttgaac gattcgaggt caagaccttc tccctcctgc
7680 acaacttctg tctggtctcc atctccgcct acatgtgcgg tggcatcctg
tacgaggctt 7740 atcaggccaa ctatggactg tttgagaacg ctgccgatca
caccttcaag ggtctcccta 7800 tggctaagat gatctggctc ttctacttct
ccaagatcat ggagtttgtc gacaccatga 7860 tcatggtcct caagaagaac
aaccgacaga tttcctttct gcacgtgtac caccactctt 7920 ccatcttcac
catctggtgg ctggtcacct tcgttgctcc caacggtgaa gcctacttct 7980
ctgctgccct gaactccttc atccacgtca tcatgtacgg ctactacttt ctgtctgccc
8040 tgggcttcaa gcaggtgtcg ttcatcaagt tctacatcac tcgatcccag
atgacccagt 8100 tctgcatgat gtctgtccag tcttcctggg acatgtacgc
catgaaggtc cttggccgac 8160 ctggataccc cttcttcatc accgctctgc
tctggttcta catgtggacc atgctcggtc 8220 tcttctacaa cttttaccga
aagaacgcca agctcgccaa gcaggccaag gctgacgctg 8280 ccaaggagaa
ggccagaaag ctccagtaag cggccgcaag tgtggatggg gaagtgagtg 8340
cccggttctg tgtgcacaat tggcaatcca agatggatgg attcaacaca gggatatagc
8400 gagctacgtg gtggtgcgag gatatagcaa cggatattta tgtttgacac
ttgagaatgt 8460 acgatacaag cactgtccaa gtacaatact aaacatactg
tacatactca tactcgtacc 8520 cgggcaacgg tttcacttga gtgcagtggc
tagtgctctt actcgtacag tgtgcaatac 8580 tgcgtatcat agtctttgat
gtatatcgta ttcattcatg ttagttgcgt acgaagtcgt 8640 caatgatgtc
gatatgggtt ttgatcatgc acacataagg tccgacctta tcggcaagct 8700
caatgagctc cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg
8760 ccacgagctt gagcactcga gcggcaaagg cggacttgtg gacgttagct
cgagcttcgt 8820 aggagggcat tttggtggtg aagaggagac tgaaataaat
ttagtctgca gaacttttta 8880 tcggaacctt atctggggca gtgaagtata
tgttatggta atagttacga gttagttgaa 8940 cttatagata gactggacta
tacggctatc ggtccaaatt agaaagaacg tcaatggctc 9000 tctgggcgtc
gcctttgccg acaaaaatgt gatcatgatg aaagccagca atgacgttgc 9060
agctgatatt gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca
9120 acgaagaatg tatcgtcaaa gtgatccaag cacactcata gttggagtcg
tactccaaag 9180 gcggcaatga cgagtcagac agatactcgt cgaccttttc
cttgggaacc accaccgtca 9240 gcccttctga ctcacgtatt gtagccaccg
acacaggcaa cagtccgtgg atagcagaat 9300 atgtcttgtc ggtccatttc
tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt 9360 atgtgccttc
attgagaatc ggtgttgctg atttcaataa agtcttgaga tcagtttggc 9420
gcgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
9480 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt 9540 atcagctcac tcaaaggcgg taatacggtt atccacagaa
tcaggggata acgcaggaaa 9600 gaacatgtga gcaaaaggcc agcaaaaggc
caggaaccgt aaaaaggccg cgttgctggc 9660 gtttttccat aggctccgcc
cccctgacga gcatcacaaa aatcgacgct caagtcagag 9720 gtggcgaaac
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 9780
gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
9840 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg 9900 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg 9960 taactatcgt cttgagtcca acccggtaag
acacgactta tcgccactgg cagcagccac 10020 tggtaacagg attagcagag
cgaggtatgt aggcggtgct acagagttct tgaagtggtg 10080 gcctaactac
ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 10140
taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
10200 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc 10260 tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt aagggatttt 10320 ggtcatgaga ttatcaaaaa ggatcttcac
ctagatcctt ttaaattaaa aatgaagttt 10380 taaatcaatc taaagtatat
atgagtaaac ttggtctgac agttaccaat gcttaatcag 10440 tgaggcacct
atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 10500
cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
10560 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc 10620 cgagcgcaga agtggtcctg caactttatc cgcctccatc
cagtctatta attgttgccg 10680 ggaagctaga gtaagtagtt cgccagttaa
tagtttgcgc aacgttgttg ccattgctac 10740 aggcatcgtg gtgtcacgct
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 10800 atcaaggcga
gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 10860
tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
10920 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc 10980 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat 11040 acgggataat accgcgccac atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc 11100 ttcggggcga aaactctcaa
ggatcttacc gctgttgaga tccagttcga tgtaacccac 11160 tcgtgcaccc
aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 11220
aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
11280 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg 11340 atacatattt gaatgtattt agaaaaataa acaaataggg
gttccgcgca catttccccg 11400 aaaagtgcca cctgatgcgg tgtgaaatac
cgcacagatg cgtaaggaga aaataccgca 11460 tcaggaaatt gtaagcgtta
atattttgtt aaaattcgcg ttaaattttt gttaaatcag 11520 ctcatttttt
aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 11580
cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
11640 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
gtgaaccatc 11700 accctaatca agttttttgg ggtcgaggtg ccgtaaagca
ctaaatcgga accctaaagg 11760 gagcccccga tttagagctt gacggggaaa
gccggcgaac gtggcgagaa aggaagggaa 11820 gaaagcgaaa ggagcgggcg
ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 11880 caccacaccc
gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg 11940
cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
12000 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
gtcacgacgt 12060 tgtaaaacga cggccagtga attgtaatac gactcactat
agggcgaatt gggcccgacg 12120 tcgcatgcag tggtggtatt gtgactgggg
atgtagttga gaataagtca tacacaagtc 12180 agctttcttc gagcctcata
taagtataag tagttcaacg tattagcact gtacccagca 12240 tctccgtatc
gagaaacaca acaacatgcc ccattggaca gatcatgcgg atacacaggt 12300
tgtgcagtat catacatact cgatcagaca ggtcgtctga ccatcataca agctgaacaa
12360 gcgctccata cttgcacgct ctctatatac acagttaaat tacatatcca
tagtctaacc 12420 tctaacagtt aatcttctgg taagcctccc agccagcctt
ctggtatcgc ttggcctcct 12480 caataggatc tcggttctgg ccgtacagac
ctcggccgac aattatgata tccgttccgg 12540 tagacatgac atcctcaaca
gttcggtact gctgtccgag agcgtctccc ttgtcgtcaa 12600 gacccacccc
gggggtcaga ataagccagt cctcagagtc gcccttaat 12649 <210> SEQ ID
NO 57 <211> LENGTH: 819 <212> TYPE: DNA <213>
ORGANISM: Thraustochytrium aureum <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: synthetic
elongase (codon-optimized) <400> SEQUENCE: 57 atggccaact
cctctgtctg ggacgacgtg gtcggacgag tcgagaccgg tgtcgaccag 60
tggatggacg gagctaagcc ctacgctctg accgacggtc tgcccatgat ggacgtctcc
120 accatgctcg ccttcgaggt cggctacatg gccatgctgc tcttcggcat
tcccatcatg 180 aagcagatgg agaagccctt cgagctgaag accatcaagc
tgctccacaa cctgttcctc 240 ttcggactgt ccctctacat gtgcgtcgag
accatccgac aggctatcct gggtggctac 300 aaggtcttcg gcaacgacat
ggagaagggc aacgagtccc acgctcaggg catgtcccga 360 atcgtctacg
tgttctacgt ctccaaggcc tacgagttcc tggacaccgc tatcatgatc 420
ctgtgcaaga agttcaacca ggtctccttc ctgcacgtgt accaccatgc caccatcttc
480 gccatctggt gggctattgc caagtacgct cctggtggcg acgcctactt
ctccgtcatc 540 ctcaactcct tcgtccacac cgtcatgtac gcctactact
tcttttcctc tcagggcttc 600 ggcttcgtca agcccatcaa gccctacatc
accactctgc agatgaccca gttcatggct 660 atgctggtgc agtccctgta
cgactacctc ttcccctgcg actaccctca ggctctggtc 720 cagctgctcg
gcgtgtacat gatcaccctg ctcgctctgt tcggcaactt ctttgtccag 780
tcctacctga agaagcccaa gaagtccaag accaactaa 819 <210> SEQ ID
NO 58 <211> LENGTH: 272 <212> TYPE: PRT <213>
ORGANISM: Thraustochytrium aureum <400> SEQUENCE: 58 Met Ala
Asn Ser Ser Val Trp Asp Asp Val Val Gly Arg Val Glu Thr 1 5 10 15
Gly Val Asp Gln Trp Met Asp Gly Ala Lys Pro Tyr Ala Leu Thr Asp 20
25 30 Gly Leu Pro Met Met Asp Val Ser Thr Met Leu Ala Phe Glu Val
Gly 35 40 45 Tyr Met Ala Met Leu Leu Phe Gly Ile Pro Ile Met Lys
Gln Met Glu 50 55 60 Lys Pro Phe Glu Leu Lys Thr Ile Lys Leu Leu
His Asn Leu Phe Leu 65 70 75 80 Phe Gly Leu Ser Leu Tyr Met Cys Val
Glu Thr Ile Arg Gln Ala Ile 85 90 95 Leu Gly Gly Tyr Lys Val Phe
Gly Asn Asp Met Glu Lys Gly Asn Glu 100 105 110 Ser His Ala Gln Gly
Met Ser Arg Ile Val Tyr Val Phe Tyr Val Ser 115 120 125 Lys Ala Tyr
Glu Phe Leu Asp Thr Ala Ile Met Ile Leu Cys Lys Lys 130 135 140 Phe
Asn Gln Val Ser Phe Leu His Val Tyr His His Ala Thr Ile Phe 145 150
155 160 Ala Ile Trp Trp Ala Ile Ala Lys Tyr Ala Pro Gly Gly Asp Ala
Tyr 165 170 175 Phe Ser Val Ile Leu Asn Ser Phe Val His Thr Val Met
Tyr Ala Tyr 180 185 190 Tyr Phe Phe Ser Ser Gln Gly Phe Gly Phe Val
Lys Pro Ile Lys Pro 195 200 205 Tyr Ile Thr Thr Leu Gln Met Thr Gln
Phe Met Ala Met Leu Val Gln 210 215 220 Ser Leu Tyr Asp Tyr Leu Phe
Pro Cys Asp Tyr Pro Gln Ala Leu Val 225 230 235 240 Gln Leu Leu Gly
Val Tyr Met Ile Thr Leu Leu Ala Leu Phe Gly Asn 245 250 255 Phe Phe
Val Gln Ser Tyr Leu Lys Lys Pro Lys Lys Ser Lys Thr Asn 260 265 270
<210> SEQ ID NO 59 <211> LENGTH: 13034 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pDMW271 <400>
SEQUENCE: 59 cgatgcagaa ttcaggagag accgggttgg cggcgtattt gtgtcccaaa
aaacagcccc 60 aattgcccca attgacccca aattgaccca gtagcgggcc
caaccccggc gagagccccc 120 ttcaccccac atatcaaacc tcccccggtt
cccacacttg ccgttaaggg cgtagggtac 180 tgcagtctgg aatctacgct
tgttcagact ttgtactagt ttctttgtct ggccatccgg 240 gtaacccatg
ccggacgcaa aatagactac tgaaaatttt tttgctttgt ggttgggact 300
ttagccaagg gtataaaaga ccaccgtccc cgaattacct ttcctcttct tttctctctc
360 tccttgtcaa ctcacacccg aaatcgttaa gcatttcctt ctgagtataa
gaatcattca 420 ccatggatgg ctcccgaccc tgtcgctgcc gagaccgctg
cccagggtcc cactccccga 480 tacttcacct gggacgaggt cgcccagcga
tccggttgcg aggaacgatg gctggtcatc 540 gaccgaaagg tgtacaacat
ctctgagttc acccgacgac atcccggtgg ctcccgagtg 600 atctcgcact
acgctggaca ggacgccact gaccccttcg ttgcctttca cattaacaag 660
ggcctggtta agaagtacat gaactccctg ctcattggag agctgtctcc cgaacagcct
720 tcgtttgagc ctaccaagaa caaggagctg accgacgagt ttcgagagct
ccgagccacc 780 gttgagcgaa tgggactgat gaaggccaac catgtcttct
ttctgctcta cctgctccac 840 attcttctcc ttgacggagc tgcctggctt
accctgtggg tcttcggcac ttcctttctg 900 ccctttcttc tctgcgccgt
cctgctctct gccgtgcagg ctcaggctgg ttggcttcag 960 catgactttg
gtcacctttc cgtgttctct acctccaagt ggaaccacct gctccatcac 1020
ttcgtgatcg gccacctcaa gggtgctcct gcctcgtggt ggaaccacat gcatttccag
1080 caccatgcca agcccaactg ttttcgaaag gatcccgaca tcaacatgca
ccccttcttt 1140 ttcgctcttg gcaagatcct gtccgtcgag ctcggaaagc
agaagaagaa gtacatgccc 1200 tacaaccacc agcacaagta cttcttcctg
attggacctc ccgctctcct gcctctttac 1260 tttcagtggt acatctttta
ctttgttatt cagcgaaaga agtgggttga tcttgcctgg 1320 atgatcacct
tctacgtccg attcttcctg acctacgtcc ctctccttgg actgaaggcc 1380
tttctcggtc tgttctttat cgtccgattc ctggagtcca actggttcgt gtgggtgacc
1440 cagatgaacc acattcccat gcacattgac catgatcgaa acatggactg
ggtgtcgact 1500 cagctgcagg ccacctgcaa cgttcacaag tctgctttca
acgactggtt ttccggtcac 1560 ctcaactttc agattgagca ccatctgttt
cccaccatgc ctcgacacaa ctaccacaag 1620 gttgctcccc tggtccagtc
gctctgtgcc aagcatggca tcgagtacca gtccaagccc 1680 ctgctctctg
ccttcgctga catcattcac tcgctgaagg aatctggcca gctctggctc 1740
gatgcctacc tgcaccagta agcggccgca ttgatgattg gaaacacaca catgggttat
1800 atctaggtga gagttagttg gacagttata tattaaatca gctatgccaa
cggtaacttc 1860 attcatgtca acgaggaacc agtgactgca agtaatatag
aatttgacca ccttgccatt 1920 ctcttgcact cctttactat atctcattta
tttcttatat acaaatcact tcttcttccc 1980 agcatcgagc tcggaaacct
catgagcaat aacatcgtgg atctcgtcaa tagagggctt 2040 tttggactcc
ttgctgttgg ccaccttgtc cttgctgtct ggctcattct gtttcaacgc 2100
cttttaatta acggagtagg tctcggtgtc ggaagcgacg ccagatccgt catcctcctt
2160 tcgctctcca aagtagatac ctccgacgag ctctcggaca atgatgaagt
cggtgccctc 2220 aacgtttcgg atgggggaga gatcggcgag cttgggcgac
agcagctggc agggtcgcag 2280 gttggcgtac aggttcaggt cctttcgcag
cttgaggaga ccctgctcgg gtcgcacgtc 2340 ggttcgtccg tcgggagtgg
tccatacggt gttggcagcg cctccgacag caccgagcat 2400 aatagagtca
gcctttcggc agatgtcgag agtagcgtcg gtgatgggct cgccctcctt 2460
ctcaatggca gctcctccaa tgagtcggtc ctcaaacaca aactcggtgc cggaggcctc
2520 agcaacagac ttgagcacct tgacggcctc ggcaatcacc tcggggccac
agaagtcgcc 2580 gccgagaaga acaatcttct tggagtcagt cttggtcttc
ttagtttcgg gttccattgt 2640 ggatgtgtgt ggttgtatgt gtgatgtggt
gtgtggagtg aaaatctgtg gctggcaaac 2700 gctcttgtat atatacgcac
ttttgcccgt gctatgtgga agactaaacc tccgaagatt 2760 gtgactcagg
tagtgcggta tcggctaggg acccaaacct tgtcgatgcc gatagcatgc 2820
gacgtcgggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
2880 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
agcacatccc 2940 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
atcgcccttc ccaacagttg 3000 cgcagcctga atggcgaatg gacgcgccct
gtagcggcgc attaagcgcg gcgggtgtgg 3060 tggttacgcg cagcgtgacc
gctacacttg ccagcgccct agcgcccgct cctttcgctt 3120 tcttcccttc
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3180
tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
3240 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
ttgacgttgg 3300 agtccacgtt ctttaatagt ggactcttgt tccaaactgg
aacaacactc aaccctatct 3360 cggtctattc ttttgattta taagggattt
tgccgatttc ggcctattgg ttaaaaaatg 3420 agctgattta acaaaaattt
aacgcgaatt ttaacaaaat attaacgctt acaatttcct 3480 gatgcggtat
tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt 3540
ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
3600 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
aggaagagta 3660 tgagtattca acatttccgt gtcgccctta ttcccttttt
tgcggcattt tgccttcctg 3720 tttttgctca cccagaaacg ctggtgaaag
taaaagatgc tgaagatcag ttgggtgcac 3780 gagtgggtta catcgaactg
gatctcaaca gcggtaagat ccttgagagt tttcgccccg 3840 aagaacgttt
tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 3900
gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
3960 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
agagaattat 4020 gcagtgctgc cataaccatg agtgataaca ctgcggccaa
cttacttctg acaacgatcg 4080 gaggaccgaa ggagctaacc gcttttttgc
acaacatggg ggatcatgta actcgccttg 4140 atcgttggga accggagctg
aatgaagcca taccaaacga cgagcgtgac accacgatgc 4200 ctgtagcaat
ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 4260
cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
4320 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
cgtgggtctc 4380 gcggtatcat tgcagcactg gggccagatg gtaagccctc
ccgtatcgta gttatctaca 4440 cgacggggag tcaggcaact atggatgaac
gaaatagaca gatcgctgag ataggtgcct 4500 cactgattaa gcattggtaa
ctgtcagacc aagtttactc atatatactt tagattgatt 4560 taaaacttca
tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 4620
ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
4680 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
acaaaaaaac 4740 caccgctacc agcggtggtt tgtttgccgg atcaagagct
accaactctt tttccgaagg 4800 taactggctt cagcagagcg cagataccaa
atactgttct tctagtgtag ccgtagttag 4860 gccaccactt caagaactct
gtagcaccgc ctacatacct cgctctgcta atcctgttac 4920 cagtggctgc
tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4980
taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
5040 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
agcgccacgc 5100 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
cagggtcgga acaggagagc 5160 gcacgaggga gcttccaggg ggaaacgcct
ggtatcttta tagtcctgtc gggtttcgcc 5220 acctctgact tgagcgtcga
tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 5280 acgccagcaa
cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 5340
tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
5400 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
gaagcggaag 5460 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
gattcattaa tgcagctggc 5520 gcgcccactg agctcgtcta acggacttga
tatacaacca attaaaacaa atgaaaagaa 5580 atacagttct ttgtatcatt
tgtaacaatt accctgtaca aactaaggta ttgaaatccc 5640 acaatattcc
caaagtccac ccctttccaa attgtcatgc ctacaactca tataccaagc 5700
actaacctac caaacaccac taaaacccca caaaatatat cttaccgaat atacagtaac
5760 aagctaccac cacactcgtt gggtgcagtc gccagcttaa agatatctat
ccacatcagc 5820 cacaactccc ttcctttaat aaaccgacta cacccttggc
tattgaggtt atgagtgaat 5880 atactgtaga caagacactt tcaagaagac
tgtttccaaa acgtaccact gtcctccact 5940 acaaacacac ccaatctgct
tcttctagtc aaggttgcta caccggtaaa ttataaatca 6000 tcatttcatt
agcagggcag ggcccttttt atagagtctt atacactagc ggaccctgcc 6060
ggtagaccaa cccgcaggcg cgtcagtttg ctccttccat caatgcgtcg tagaaacgac
6120 ttactccttc ttgagcagct ccttgacctt gttggcaaca agtctccgac
ctcggaggtg 6180 gaggaagagc ctccgatatc ggcggtagtg ataccagcct
cgacggactc cttgacggca 6240 gcctcaacag cgtcaccggc gggcttcatg
ttaagagaga acttgagcat catggcggca 6300 gacagaatgg tggcgtacgc
aactaacatg aatgaatacg atatacatca aagactatga 6360 tacgcagtat
tgcacactgt acgagtaaga gcactagcca ctgcactcaa gtgaaaccgt 6420
tgcccgggta cgagtatgag tatgtacagt atgtttagta ttgtacttgg acagtgcttg
6480 tatcgtacat tctcaagtgt caaacataaa tatccgttgc tatatcctcg
caccaccacg 6540 tagctcgcta tatccctgtg ttgaatccat ccatcttgga
ttgccaattg tgcacacaga 6600 accgggcact cacttcccca tccacacttg
cggccgctta gctgcctact cttccttggg 6660 acggagtcca agaacacgca
agtgctccaa atgtgaagca aatgcttgcc aaaacgtatc 6720 cttgacaagg
tatggaacct tgtactcgct gcaggtgttc ttgatgatgg ccagaatatc 6780
gggataatgg tgctgcgaca cgttggggaa cagatggtgc acagcctggt agttcaagct
6840 gccagtgatg ctggtccaga ggtgcgaatc gtgtgcgtaa tcctgcgtag
tctcgacctg 6900 catagctgcc cagtcctttt ggatgatccc gttctcgtca
ggcaacggcc actgaacttc 6960 ctcaacaacg tggttcgcct ggaaggtcag
cgccagccag taagacgaca ccatgtccgc 7020 gaccgtgaac aagagcagca
ccttgcccag gggcagatac tgcaggggaa caatcaggcg 7080 ataccagaca
aagaaagcct tgccgcccca gaacatcaca gtgtgccatg tcgagatggg 7140
attgacacga atagcgtcat tggtcttgac aaagtacaaa atgttgatgt cctgaatgcg
7200 caccttgaac gccagcagtc cgtacaggaa aggaacaaac atgtgctggt
tgatgtggtt 7260 gacaaaccac ttttggttgg gcttgatacg acgaacatcg
ggctcagacg tcgacacgtc 7320 gggatctgct ccagcaatgt tggtgtaggg
gtgatggccg agcatatgtt ggtacatcca 7380 caccaggtac gatgctccgt
tgaaaaagtc gtgcgtggct cccagaatct tccagacagt 7440 ggggttgtgg
gtcactgaaa agtgagacgc atcatgaaga gggttgagtc cgacttgtgc 7500
gcacgcaaat cccatgatga ttgcaaacac cacctgaagc catgtgcgtt cgacaacgaa
7560 aggcacaaag agctgcgcgt agtaggaagc gatcaaggat ccaaagataa
gagcgtatcg 7620 tccccagatc tctggtctat tcttgggatc aatgttccga
tccgtaaagt agccctcgac 7680 tctcgtcttg atggttttgt ggaacaccgt
tggctccggg aagatgggca gctcattcga 7740 gaccagtgta ccgacatagt
acttcttcat aatggcatct gcagccccaa acgcgtgata 7800 catctcaaag
accggagtaa catctcggcc agctccgagc aggagagtgt ccactccacc 7860
aggatggcgg ctcaagaact ttgtgacatc gtacaccctg ccgcggatgg ccaagagtag
7920 gtcgtccttg gtgttatggg ccgccagctc ttcccaggtg aaggtttttc
cttggtccgt 7980 tcccatggag agctgggtta gtttgtgtag agagtgtgtg
ttgctagcga ctttcggatt 8040 gtgtcattac acaaaacgcg tcgtctcgac
actgatcttg tcgtggatac tcacggctcg 8100 gacatcgtcg ccgacgatga
caccggactt tcgcttaagg acgtcagtaa caggcattgt 8160 gtgatgtgta
gtttagattt cgaatctgtg gggaaagaaa ggaaaaaaga gactggcaac 8220
cgattgggag agccactgtt tatatatacc ctagacaagc cccccgcttg taagatgttg
8280 gtcaatgtaa accagtatta aggttggcaa gtgcaggaga agcaaggtgt
gggtaccgag 8340 caatggaaat gtgcggaagg caaaaaaatg aggccacggc
ctattgtcgg ggctatatcc 8400 agggggcgat tgaagtacac taacatgaca
tgtgtccaca gaccctcaat ctggcctgat 8460 gagccaaatc catacgcgct
ttcgcagctc taaaggctat aacaagtcac accaccctgc 8520 tcgacctcag
cgccctcact ttttgttaag acaaactgta cacgctgttc cagcgttttc 8580
tgcctgcacc tggtgggaca tttggtgcaa cctaaagtgc tcggaacctc tgtggtgtcc
8640 agatcagcgc agcagttccg aggtagtttt gaggccctta gatgatgcaa
tggtgtcagt 8700 cgctggatca cgagtcttaa tggcagtatt cgttcttatt
tgtgccattg agccccgtta 8760 tcctcgtatc ttctaccccc catcccatcc
ctttgttggt gcaaccctac ccatttattg 8820 ttgggtgcag cccaaccgac
gtggagagct tggcttggcc atataaaaag gcccccccct 8880 agtggcaatg
gcagaaagtc agctgtgagt tgttgaattt gtcatctagg cggcctggcc 8940
gtcttctccg gggcaattta aattccttca cttcaagttc attcttcatc tgcttctgtt
9000 ttactttgac aggcaaatga agacatggta cgacttgatg gaggccaaga
acgccatttc 9060 accccgagac accgaagtgc ctgaaatcct ggctgccccc
attgataaca tcggaaacta 9120 cggtattccg gaaagtgtat atagaacctt
tccccagctt gtgtctgtgg atatggatgg 9180 tgtaatcccc tttgagtact
cgtcttggct tctctccgag cagtatgagg ctctctaatc 9240 tagcgcattt
aatatctcaa tgtatttata tatttatctt ctcatgcggc cgcttagctg 9300
cctactcttc cttgggacgg agtccaagaa cacgcaagtg ctccaaatgt gaagcaaatg
9360 cttgccaaaa cgtatccttg acaaggtatg gaaccttgta ctcgctgcag
gtgttcttga 9420 tgatggccag aatatcggga taatggtgct gcgacacgtt
ggggaacaga tggtgcacag 9480 cctggtagtt caagctgcca gtgatgctgg
tccagaggtg cgaatcgtgt gcgtaatcct 9540 gcgtagtctc gacctgcata
gctgcccagt ccttttggat gatcccgttc tcgtcaggca 9600 acggccactg
aacttcctca acaacgtggt tcgcctggaa ggtcagcgcc agccagtaag 9660
acgacaccat gtccgcgacc gtgaacaaga gcagcacctt gcccaggggc agatactgca
9720 ggggaacaat caggcgatac cagacaaaga aagccttgcc gccccagaac
atcacagtgt 9780 gccatgtcga gatgggattg acacgaatag cgtcattggt
cttgacaaag tacaaaatgt 9840 tgatgtcctg aatgcgcacc ttgaacgcca
gcagtccgta caggaaagga acaaacatgt 9900 gctggttgat gtggttgaca
aaccactttt ggttgggctt gatacgacga acatcgggct 9960 cagacgtcga
cacgtcggga tctgctccag caatgttggt gtaggggtga tggccgagca 10020
tatgttggta catccacacc aggtacgatg ctccgttgaa aaagtcgtgc gtggctccca
10080 gaatcttcca gacagtgggg ttgtgggtca ctgaaaagtg agacgcatca
tgaagagggt 10140 tgagtccgac ttgtgcgcac gcaaatccca tgatgattgc
aaacaccacc tgaagccatg 10200 tgcgttcgac aacgaaaggc acaaagagct
gcgcgtagta ggaagcgatc aaggatccaa 10260 agataagagc gtatcgtccc
cagatctctg gtctattctt gggatcaatg ttccgatccg 10320 taaagtagcc
ctcgactctc gtcttgatgg ttttgtggaa caccgttggc tccgggaaga 10380
tgggcagctc attcgagacc agtgtaccga catagtactt cttcataatg gcatctgcag
10440 ccccaaacgc gtgatacatc tcaaagaccg gagtaacatc tcggccagct
ccgagcagga 10500 gagtgtccac tccaccagga tggcggctca agaactttgt
gacatcgtac accctgccgc 10560 ggatggccaa gagtaggtcg tccttggtgt
tatgggccgc cagctcttcc caggtgaagg 10620 tttttccttg gtccgttccc
atggtgaatg attcttatac tcagaaggaa atgcttaacg 10680 atttcgggtg
tgagttgaca aggagagaga gaaaagaaga ggaaaggtaa ttcggggacg 10740
gtggtctttt atacccttgg ctaaagtccc aaccacaaag caaaaaaatt ttcagtagtc
10800 tattttgcgt ccggcatggg ttacccggat ggccagacaa agaaactagt
acaaagtctg 10860 aacaagcgta gattccagac tgcagtaccc tacgccctta
acggcaagtg tgggaaccgg 10920 gggaggtttg atatgtgggg tgaagggggc
tctcgccggg gttgggcccg ctactgggtc 10980 aatttggggt caattggggc
aattggggct gttttttggg acacaaatac gccgccaacc 11040 cggtctctcc
tgatcgatgg gctgcaggaa ttctacaata cgtgagtcag aagggctgac 11100
ggtggtggtt cccaaggaaa aggtcgacga gtatctgtct gactcgtcat tgccgccttt
11160 ggagtacgac tccaactatg agtgtgcttg gatcactttg acgatacatt
cttcgttgga 11220 ggctgtgggt ctgacagctg cgttttcggc gcggttggcc
gacaacaata tcagctgcaa 11280 cgtcattgct ggctttcatc atgatcacat
ttttgtcggc aaaggcgacg cccagagagc 11340 cattgacgtt ctttctaatt
tggaccgata gccgtatagt ccagtctatc tataagttca 11400 actaactcgt
aactattacc ataacatata cttcactgcc ccagataagg ttccgataaa 11460
aagttctgca gactaaattt atttcagtct cctcttcacc accaaaatgc cctcctacga
11520 agctcgagct aacgtccaca agtccgcctt tgccgctcga gtgctcaagc
tcgtggcagc 11580 caagaaaacc aacctgtgtg cttctctgga tgttaccacc
accaaggagc tcattgagct 11640 tgccgataag gtcggacctt atgtgtgcat
gatcaaaacc catatcgaca tcattgacga 11700 cttcacctac gccggcactg
tgctccccct caaggaactt gctcttaagc acggtttctt 11760 cctgttcgag
gacagaaagt tcgcagatat tggcaacact gtcaagcacc agtaccggtg 11820
tcaccgaatc gccgagtggt ccgatatcac caacgcccac ggtgtacccg gaaccggaat
11880 cattgctggc ctgcgagctg gtgccgagga aactgtctct gaacagaaga
aggaggacgt 11940 ctctgactac gagaactccc agtacaagga gttcctagtc
ccctctccca acgagaagct 12000 ggccagaggt ctgctcatgc tggccgagct
gtcttgcaag ggctctctgg ccactggcga 12060 gtactccaag cagaccattg
agcttgcccg atccgacccc gagtttgtgg ttggcttcat 12120 tgcccagaac
cgacctaagg gcgactctga ggactggctt attctgaccc ccggggtggg 12180
tcttgacgac aagggagacg ctctcggaca gcagtaccga actgttgagg atgtcatgtc
12240 taccggaacg gatatcataa ttgtcggccg aggtctgtac ggccagaacc
gagatcctat 12300 tgaggaggcc aagcgatacc agaaggctgg ctgggaggct
taccagaaga ttaactgtta 12360 gaggttagac tatggatatg taatttaact
gtgtatatag agagcgtgca agtatggagc 12420 gcttgttcag cttgtatgat
ggtcagacga cctgtctgat cgagtatgta tgatactgca 12480 caacctgtgt
atccgcatga tctgtccaat ggggcatgtt gttgtgtttc tcgatacgga 12540
gatgctgggt acagtgctaa tacgttgaac tacttatact tatatgaggc tcgaagaaag
12600 ctgacttgtg tatgacttat tctcaactac atccccagtc acaataccac
cactgcacta 12660 ccactacacc agatctgcgt acactgttta aacggtaggt
tagtgcttgg tatatgagtt 12720 gtaggcatga caatttggaa aggggtggac
tttgggaata ttgtgggatt tcaatacctt 12780 agtttgtaca gggtaattgt
tacaaatgat acaaagaact gtatttcttt tcatttgttt 12840 taattggttg
tatatcaagt ccgttagacg agctcagtgc cttggctttt ggcactgtat 12900
ttcattttta gaggtacact acattcagtg aggtatggta aggttgaggg cataatgaag
12960 gcaccttgta ctgacagtca cagacctctc accgagaatt ttatgagata
tactcgggtt 13020 cattttaggc tcat 13034 <210> SEQ ID NO 60
<211> LENGTH: 1335 <212> TYPE: DNA <213>
ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY:
misc_feature <223> OTHER INFORMATION: synthetic delta-5
desaturase (codon-optimized) <400> SEQUENCE: 60 atggctcccg
accctgtcgc tgccgagacc gctgcccagg gtcccactcc ccgatacttc 60
acctgggacg aggtcgccca gcgatccggt tgcgaggaac gatggctggt catcgaccga
120 aaggtgtaca acatctctga gttcacccga cgacatcccg gtggctcccg
agtgatctcg 180 cactacgctg gacaggacgc cactgacccc ttcgttgcct
ttcacattaa caagggcctg 240 gttaagaagt acatgaactc cctgctcatt
ggagagctgt ctcccgaaca gccttcgttt 300 gagcctacca agaacaagga
gctgaccgac gagtttcgag agctccgagc caccgttgag 360 cgaatgggac
tgatgaaggc caaccatgtc ttctttctgc tctacctgct ccacattctt 420
ctccttgacg gagctgcctg gcttaccctg tgggtcttcg gcacttcctt tctgcccttt
480 cttctctgcg ccgtcctgct ctctgccgtg caggctcagg ctggttggct
tcagcatgac 540 tttggtcacc tttccgtgtt ctctacctcc aagtggaacc
acctgctcca tcacttcgtg 600 atcggccacc tcaagggtgc tcctgcctcg
tggtggaacc acatgcattt ccagcaccat 660 gccaagccca actgttttcg
aaaggatccc gacatcaaca tgcacccctt ctttttcgct 720 cttggcaaga
tcctgtccgt cgagctcgga aagcagaaga agaagtacat gccctacaac 780
caccagcaca agtacttctt cctgattgga cctcccgctc tcctgcctct ttactttcag
840 tggtacatct tttactttgt tattcagcga aagaagtggg ttgatcttgc
ctggatgatc 900 accttctacg tccgattctt cctgacctac gtccctctcc
ttggactgaa ggcctttctc 960 ggtctgttct ttatcgtccg attcctggag
tccaactggt tcgtgtgggt gacccagatg 1020 aaccacattc ccatgcacat
tgaccatgat cgaaacatgg actgggtgtc gactcagctg 1080 caggccacct
gcaacgttca caagtctgct ttcaacgact ggttttccgg tcacctcaac 1140
tttcagattg agcaccatct gtttcccacc atgcctcgac acaactacca caaggttgct
1200 cccctggtcc agtcgctctg tgccaagcat ggcatcgagt accagtccaa
gcccctgctc 1260 tctgccttcg ctgacatcat tcactcgctg aaggaatctg
gccagctctg gctcgatgcc 1320 tacctgcacc agtaa 1335 <210> SEQ ID
NO 61 <211> LENGTH: 444 <212> TYPE: PRT <213>
ORGANISM: Homo sapiens <400> SEQUENCE: 61 Met Ala Pro Asp Pro
Val Ala Ala Glu Thr Ala Ala Gln Gly Pro Thr 1 5 10 15 Pro Arg Tyr
Phe Thr Trp Asp Glu Val Ala Gln Arg Ser Gly Cys Glu 20 25 30 Glu
Arg Trp Leu Val Ile Asp Arg Lys Val Tyr Asn Ile Ser Glu Phe 35 40
45 Thr Arg Arg His Pro Gly Gly Ser Arg Val Ile Ser His Tyr Ala Gly
50 55 60 Gln Asp Ala Thr Asp Pro Phe Val Ala Phe His Ile Asn Lys
Gly Leu 65 70 75 80 Val Lys Lys Tyr Met Asn Ser Leu Leu Ile Gly Glu
Leu Ser Pro Glu 85 90 95 Gln Pro Ser Phe Glu Pro Thr Lys Asn Lys
Glu Leu Thr Asp Glu Phe 100 105 110 Arg Glu Leu Arg Ala Thr Val Glu
Arg Met Gly Leu Met Lys Ala Asn 115 120 125 His Val Phe Phe Leu Leu
Tyr Leu Leu His Ile Leu Leu Leu Asp Gly 130 135 140 Ala Ala Trp Leu
Thr Leu Trp Val Phe Gly Thr Ser Phe Leu Pro Phe 145 150 155 160 Leu
Leu Cys Ala Val Leu Leu Ser Ala Val Gln Ala Gln Ala Gly Trp 165 170
175 Leu Gln His Asp Phe Gly His Leu Ser Val Phe Ser Thr Ser Lys Trp
180 185 190 Asn His Leu Leu His His Phe Val Ile Gly His Leu Lys Gly
Ala Pro 195 200 205 Ala Ser Trp Trp Asn His Met His Phe Gln His His
Ala Lys Pro Asn 210 215 220 Cys Phe Arg Lys Asp Pro Asp Ile Asn Met
His Pro Phe Phe Phe Ala 225 230 235 240 Leu Gly Lys Ile Leu Ser Val
Glu Leu Gly Lys Gln Lys Lys Lys Tyr 245 250 255 Met Pro Tyr Asn His
Gln His Lys Tyr Phe Phe Leu Ile Gly Pro Pro 260 265 270 Ala Leu Leu
Pro Leu Tyr Phe Gln Trp Tyr Ile Phe Tyr Phe Val Ile 275 280 285 Gln
Arg Lys Lys Trp Val Asp Leu Ala Trp Met Ile Thr Phe Tyr Val 290 295
300 Arg Phe Phe Leu Thr Tyr Val Pro Leu Leu Gly Leu Lys Ala Phe Leu
305 310 315 320 Gly Leu Phe Phe Ile Val Arg Phe Leu Glu Ser Asn Trp
Phe Val Trp 325 330 335 Val Thr Gln Met Asn His Ile Pro Met His Ile
Asp His Asp Arg Asn 340 345 350 Met Asp Trp Val Ser Thr Gln Leu Gln
Ala Thr Cys Asn Val His Lys 355 360 365 Ser Ala Phe Asn Asp Trp Phe
Ser Gly His Leu Asn Phe Gln Ile Glu 370 375 380 His His Leu Phe Pro
Thr Met Pro Arg His Asn Tyr His Lys Val Ala 385 390 395 400 Pro Leu
Val Gln Ser Leu Cys Ala Lys His Gly Ile Glu Tyr Gln Ser 405 410 415
Lys Pro Leu Leu Ser Ala Phe Ala Asp Ile Ile His Ser Leu Lys Glu 420
425 430 Ser Gly Gln Leu Trp Leu Asp Ala Tyr Leu His Gln 435 440
<210> SEQ ID NO 62 <211> LENGTH: 3800 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pPaD17S <400>
SEQUENCE: 62
ggccgcatcg gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc
60 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
acaacatacg 120 agccggaagc ataaagtgta aagcctgggg tgcctaatga
gtgagctaac tcacattaat 180 tgcgttgcgc tcactgcccg ctttccagtc
gggaaacctg tcgtgccagc tgcattaatg 240 aatcggccaa cgcgcgggga
gaggcggttt gcgtattggg cgctcttccg cttcctcgct 300 cactgactcg
ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 360
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
420 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
ataggctccg 480 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
aggtggcgaa acccgacagg 540 actataaaga taccaggcgt ttccccctgg
aagctccctc gtgcgctctc ctgttccgac 600 cctgccgctt accggatacc
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 660 tagctcacgc
tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 720
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
780 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
ggattagcag 840 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
tggcctaact acggctacac 900 tagaagaaca gtatttggta tctgcgctct
gctgaagcca gttaccttcg gaaaaagagt 960 tggtagctct tgatccggca
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1020 gcagcagatt
acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1080
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
1140 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
tctaaagtat 1200 atatgagtaa acttggtctg acagttacca atgcttaatc
agtgaggcac ctatctcagc 1260 gatctgtcta tttcgttcat ccatagttgc
ctgactcccc gtcgtgtaga taactacgat 1320 acgggagggc ttaccatctg
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1380 ggctccagat
ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1440
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
1500 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
tggtgtcacg 1560 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
cgatcaaggc gagttacatg 1620 atcccccatg ttgtgcaaaa aagcggttag
ctccttcggt cctccgatcg ttgtcagaag 1680 taagttggcc gcagtgttat
cactcatggt tatggcagca ctgcataatt ctcttactgt 1740 catgccatcc
gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 1800
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
1860 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
gaaaactctc 1920 aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc 1980 ttcagcatct tttactttca ccagcgtttc
tgggtgagca aaaacaggaa ggcaaaatgc 2040 cgcaaaaaag ggaataaggg
cgacacggaa atgttgaata ctcatactct tcctttttca 2100 atattattga
agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2160
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
2220 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
cgaggccctt 2280 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
cacatgcagc tcccggagac 2340 ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc 2400 gggtgttggc gggtgtcggg
gctggcttaa ctatgcggca tcagagcaga ttgtactgag 2460 agtgcaccat
atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 2520
gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
2580 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
gggtaacgcc 2640 agggttttcc cagtcacgac gttgtaaaac gacggccagt
gaattcgagc tcggtacctc 2700 gcgaatgcat ctagatccat ggcttcctct
accgttgccg ctccctacga gttccctact 2760 ctcaccgaga tcaagcgatc
cctgcctgcc cactgcttcg aagcctctgt tccctggtcc 2820 ctctactata
ccgtgcgagc tctgggcatt gccggttccc ttgctctcgg actgtactat 2880
gctcgagccc ttgctatcgt gcaggagttt gcactgctcg atgccgtcct ttgcactggc
2940 tacattctgc tccagggtat cgtgttctgg ggattcttta ccatcggtca
cgactgtgga 3000 catggtgcct tctcgcgatc ccacctgctc aacttctctg
ttggcacact cattcactcc 3060 atcattctga ctccctacga gtcgtggaag
atcagccatc gacaccatca caagaacacc 3120 ggcaacatcg acaaggatga
gatcttctac cctcagcgag aagccgactc tcatcccctg 3180 tcccgacaca
tggtcatctc ccttggttcg gcttggtttg cctacctcgt tgctggattt 3240
cctccccgaa aggtcaacca cttcaatccc tgggagcctc tctacctgcg aagaatgtct
3300 gccgtcatca tttccctcgg ctctctcgtg gcctttgctg gtctgtacgc
ctaccttacc 3360 tacgtctacg gcctcaagac catggctctg tattacttcg
cacctctctt tggattcgcc 3420 accatgctgg ttgtcactac cttcctccat
cacaacgacg aggaaactcc ctggtacgcc 3480 gattcggagt ggacctatgt
caagggcaac ttgtcctctg tggaccgaag ctacggagcc 3540 ctcatcgaca
acctgtccca caacattggt acacatcaga tccaccatct gtttcccatc 3600
attcctcact acaagctcaa cgaggccact gctgccttcg ctcaggcctt tcccgaactg
3660 gtgcgaaagt cggcttctcc catcattccc accttcatcc gaattggtct
tatgtacgcc 3720 aagtacggcg tggtcgacaa ggatgccaag atgtttaccc
tcaaggaggc caaggctgcc 3780 aagaccaaag ccaactaagc 3800 <210>
SEQ ID NO 63 <211> LENGTH: 14655 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pZKLeuN-29E3 <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(8822)..(8822) <223> OTHER INFORMATION: n is a, c, g, or t
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (8827)..(8830) <223> OTHER INFORMATION: n is a, c,
g, or t <400> SEQUENCE: 63 cgattgttgt ctactaacta tcgtacgata
acttcgtata gcatacatta tacgaagtta 60 tcgcgtcgac gagtatctgt
ctgactcgtc attgccgcct ttggagtacg actccaacta 120 tgagtgtgct
tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc 180
tgcgttttcg gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca
240 tcatgatcac atttttgtcg gcaaaggcga cgcccagaga gccattgacg
ttctttctaa 300 tttggaccga tagccgtata gtccagtcta tctataagtt
caactaactc gtaactatta 360 ccataacata tacttcactg ccccagataa
ggttccgata aaaagttctg cagactaaat 420 ttatttcagt ctcctcttca
ccaccaaaat gccctcctac gaagctcgag ctaacgtcca 480 caagtccgcc
tttgccgctc gagtgctcaa gctcgtggca gccaagaaaa ccaacctgtg 540
tgcttctctg gatgttacca ccaccaagga gctcattgag cttgccgata aggtcggacc
600 ttatgtgtgc atgatcaaaa cccatatcga catcattgac gacttcacct
acgccggcac 660 tgtgctcccc ctcaaggaac ttgctcttaa gcacggtttc
ttcctgttcg aggacagaaa 720 gttcgcagat attggcaaca ctgtcaagca
ccagtaccgg tgtcaccgaa tcgccgagtg 780 gtccgatatc accaacgccc
acggtgtacc cggaaccgga atcattgctg gcctgcgagc 840 tggtgccgag
gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc 900
ccagtacaag gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat
960 gctggccgag ctgtcttgca agggctctct ggccactggc gagtactcca
agcagaccat 1020 tgagcttgcc cgatccgacc ccgagtttgt ggttggcttc
attgcccaga accgacctaa 1080 gggcgactct gaggactggc ttattctgac
ccccggggtg ggtcttgacg acaagggaga 1140 cgctctcgga cagcagtacc
gaactgttga ggatgtcatg tctaccggaa cggatatcat 1200 aattgtcggc
cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata 1260
ccagaaggct ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata
1320 tgtaatttaa ctgtgtatat agagagcgtg caagtatgga gcgcttgttc
agcttgtatg 1380 atggtcagac gacctgtctg atcgagtatg tatgatactg
cacaacctgt gtatccgcat 1440 gatctgtcca atggggcatg ttgttgtgtt
tctcgatacg gagatgctgg gtacagtgct 1500 aatacgttga actacttata
cttatatgag gctcgaagaa agctgacttg tgtatgactt 1560 attctcaact
acatccccag tcacaatacc accactgcac taccactaca ccaaaaccat 1620
gatcaaacca cccatggact tcctggaggc agaagaactt gttatggaaa agctcaagag
1680 agagatcata acttcgtata gcatacatta tacgaagtta tcctgcaggt
aaaggaattc 1740 tggagtttct gagagaaaaa ggcaagatac gtatgtaaca
aagcgacgca tggtacaata 1800 ataccggagg catgtatcat agagagttag
tggttcgatg atggcactgg tgcctggtat 1860 gactttatac ggctgactac
atatttgtcc tcagacatac aattacagtc aagcacttac 1920 ccttggacat
ctgtaggtac cccccggcca agacgatctc agcgtgtcgt atgtcggatt 1980
ggcgtagctc cctcgctcgt caattggctc ccatctactt tcttctgctt ggctacaccc
2040 agcatgtctg ctatggctcg ttttcgtgcc ttatctatcc tcccagtatt
accaactcta 2100 aatgacatga tgtgattggg tctacacttt catatcagag
ataaggagta gcacagttgc 2160 ataaaaagcc caactctaat cagcttcttc
ctttcttgta attagtacaa aggtgattag 2220 cgaaatctgg aagcttagtt
ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga 2280 aaaaccacag
ttttgagaac agggaggtaa cgaaggatcg tatatatata tatatatata 2340
tatacccacg gatcccgaga ccggcctttg attcttccct acaaccaacc attctcacca
2400 ccctaattca caaccatgga gtctggaccc atgcctgctg gcattccctt
ccctgagtac 2460 tatgacttct ttatggactg gaagactccc ctggccatcg
ctgccaccta cactgctgcc 2520 gtcggtctct tcaaccccaa ggttggcaag
gtctcccgag tggttgccaa gtcggctaac 2580 gcaaagcctg ccgagcgaac
ccagtccgga gctgccatga ctgccttcgt ctttgtgcac 2640 aacctcattc
tgtgtgtcta ctctggcatc accttctact acatgtttcc tgctatggtc 2700
aagaacttcc gaacccacac actgcacgaa gcctactgcg acacggatca gtccctctgg
2760 aacaacgcac ttggctactg gggttacctc ttctacctgt ccaagttcta
cgaggtcatt 2820 gacaccatca tcatcatcct gaagggacga cggtcctcgc
tgcttcagac ctaccaccat 2880 gctggagcca tgattaccat gtggtctggc
atcaactacc aagccactcc catttggatc 2940 tttgtggtct tcaactcctt
cattcacacc atcatgtact gttactatgc cttcacctct 3000 atcggattcc
atcctcctgg caaaaagtac ctgacttcga tgcagattac tcagtttctg 3060
gtcggtatca ccattgccgt gtcctacctc ttcgttcctg gctgcatccg aacacccggt
3120 gctcagatgg ctgtctggat caacgtcggc tacctgtttc ccttgaccta
tctgttcgtg 3180
gactttgcca agcgaaccta ctccaagcga tctgccattg ccgctcagaa aaaggctcag
3240 taagcggccg cattgatgat tggaaacaca cacatgggtt atatctaggt
gagagttagt 3300 tggacagtta tatattaaat cagctatgcc aacggtaact
tcattcatgt caacgaggaa 3360 ccagtgactg caagtaatat agaatttgac
caccttgcca ttctcttgca ctcctttact 3420 atatctcatt tatttcttat
atacaaatca cttcttcttc ccagcatcga gctcggaaac 3480 ctcatgagca
ataacatcgt ggatctcgtc aatagagggc tttttggact ccttgctgtt 3540
ggccaccttg tccttgctgt ctggctcatt ctgtttcaac gccttttaat taacggagta
3600 ggtctcggtg tcggaagcga cgccagatcc gtcatcctcc tttcgctctc
caaagtagat 3660 acctccgacg agctctcgga caatgatgaa gtcggtgccc
tcaacgtttc ggatggggga 3720 gagatcggcg agcttgggcg acagcagctg
gcagggtcgc aggttggcgt acaggttcag 3780 gtcctttcgc agcttgagga
gaccctgctc gggtcgcacg tcggttcgtc cgtcgggagt 3840 ggtccatacg
gtgttggcag cgcctccgac agcaccgagc ataatagagt cagcctttcg 3900
gcagatgtcg agagtagcgt cggtgatggg ctcgccctcc ttctcaatgg cagctcctcc
3960 aatgagtcgg tcctcaaaca caaactcggt gccggaggcc tcagcaacag
acttgagcac 4020 cttgacggcc tcggcaatca cctcggggcc acagaagtcg
ccgccgagaa gaacaatctt 4080 cttggagtca gtcttggtct tcttagtttc
gggttccatt gtggatgtgt gtggttgtat 4140 gtgtgatgtg gtgtgtggag
tgaaaatctg tggctggcaa acgctcttgt atatatacgc 4200 acttttgccc
gtgctatgtg gaagactaaa cctccgaaga ttgtgactca ggtagtgcgg 4260
tatcggctag ggacccaaac cttgtcgatg ccgatagcat gcgacgtcgg gcccaattcg
4320 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
tgactgggaa 4380 aaccctggcg ttacccaact taatcgcctt gcagcacatc
cccctttcgc cagctggcgt 4440 aatagcgaag aggcccgcac cgatcgccct
tcccaacagt tgcgcagcct gaatggcgaa 4500 tggacgcgcc ctgtagcggc
gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4560 ccgctacact
tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 4620
ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4680 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
tcacgtagtg 4740 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
ggagtccacg ttctttaata 4800 gtggactctt gttccaaact ggaacaacac
tcaaccctat ctcggtctat tcttttgatt 4860 tataagggat tttgccgatt
tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 4920 ttaacgcgaa
ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct 4980
tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg
5040 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
atgagacaat 5100 aaccctgata aatgcttcaa taatattgaa aaaggaagag
tatgagtatt caacatttcc 5160 gtgtcgccct tattcccttt tttgcggcat
tttgccttcc tgtttttgct cacccagaaa 5220 cgctggtgaa agtaaaagat
gctgaagatc agttgggtgc acgagtgggt tacatcgaac 5280 tggatctcaa
cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 5340
tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5400 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
tcaccagtca 5460 cagaaaagca tcttacggat ggcatgacag taagagaatt
atgcagtgct gccataacca 5520 tgagtgataa cactgcggcc aacttacttc
tgacaacgat cggaggaccg aaggagctaa 5580 ccgctttttt gcacaacatg
ggggatcatg taactcgcct tgatcgttgg gaaccggagc 5640 tgaatgaagc
cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 5700
cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
5760 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
ccggctggct 5820 ggtttattgc tgataaatct ggagccggtg agcgtgggtc
tcgcggtatc attgcagcac 5880 tggggccaga tggtaagccc tcccgtatcg
tagttatcta cacgacgggg agtcaggcaa 5940 ctatggatga acgaaataga
cagatcgctg agataggtgc ctcactgatt aagcattggt 6000 aactgtcaga
ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 6060
ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6120 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
tcttgagatc 6180 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
accaccgcta ccagcggtgg 6240 tttgtttgcc ggatcaagag ctaccaactc
tttttccgaa ggtaactggc ttcagcagag 6300 cgcagatacc aaatactgtt
cttctagtgt agccgtagtt aggccaccac ttcaagaact 6360 ctgtagcacc
gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 6420
gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6480 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
acctacaccg 6540 aactgagata cctacagcgt gagctatgag aaagcgccac
gcttcccgaa gggagaaagg 6600 cggacaggta tccggtaagc ggcagggtcg
gaacaggaga gcgcacgagg gagcttccag 6660 ggggaaacgc ctggtatctt
tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 6720 gatttttgtg
atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 6780
ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
6840 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
cgccgcagcc 6900 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
agagcgccca atacgcaaac 6960 cgcctctccc cgcgcgttgg ccgattcatt
aatgcagctg gcgcgcccac tgagctcgtc 7020 taacggactt gatatacaac
caattaaaac aaatgaaaag aaatacagtt ctttgtatca 7080 tttgtaacaa
ttaccctgta caaactaagg tattgaaatc ccacaatatt cccaaagtcc 7140
acccctttcc aaattgtcat gcctacaact catataccaa gcactaacct accaaacacc
7200 actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc
accacactcg 7260 ttgggtgcag tcgccagctt aaagatatct atccacatca
gccacaactc ccttccttta 7320 ataaaccgac tacacccttg gctattgagg
ttatgagtga atatactgta gacaagacac 7380 tttcaagaag actgtttcca
aaacgtacca ctgtcctcca ctacaaacac acccaatctg 7440 cttcttctag
tcaaggttgc tacaccggta aattataaat catcatttca ttagcagggc 7500
agggcccttt ttatagagtc ttatacacta gcggaccctg ccggtagacc aacccgcagg
7560 cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct
tcttgagcag 7620 ctccttgacc ttgttggcaa caagtctccg acctcggagg
tggaggaaga gcctccgata 7680 tcggcggtag tgataccagc ctcgacggac
tccttgacgg cagcctcaac agcgtcaccg 7740 gcgggcttca tgttaagaga
gaacttgagc atcatggcgg cagacagaat ggtggcgtac 7800 gcaactaaca
tgaatgaata cgatatacat caaagactat gatacgcagt attgcacact 7860
gtacgagtaa gagcactagc cactgcactc aagtgaaacc gttgcccggg tacgagtatg
7920 agtatgtaca gtatgtttag tattgtactt ggacagtgct tgtatcgtac
attctcaagt 7980 gtcaaacata aatatccgtt gctatatcct cgcaccacca
cgtagctcgc tatatccctg 8040 tgttgaatcc atccatcttg gattgccaat
tgtgcacaca gaaccgggca ctcacttccc 8100 catccacact tgcggccgcg
cctacttaag caacgggctt gataacagcg gggggggtgc 8160 ccacgttgtt
gcggttgcgg aagaacagaa cacccttacc agcaccctcg gcaccagcgc 8220
tgggctcaac ccactggcac atacgcgcac tgcggtacat ggcgcggatg aagccacgag
8280 gaccatcctg gacatcagcc cggtagtgct tgcccatgat gggcttaatg
gcctcggtgg 8340 cctcgtccgc gttgtagaag gggatgctgc tgacgtagtg
gtggaggaca tgagtctcga 8400 tgatgccgtg gagaaggtgg cggccgatga
agcccatctc acggtcaatg gtagcagcgg 8460 caccacggac gaagttccac
tcgtcgttgg tgtagtgggg aagggtaggg tcggtgtgct 8520 ggaggaaggt
gatggcaacg agccagtggt taacccagag gtagggaaca aagtaccaga 8580
tggccatgtt gtagaaaccg aacttctgaa cgaggaagta cagagcagtg gccatcagac
8640 cgataccaat atcgctgagg acgatgagct tagcgtcact gttctcgtac
agagggctgc 8700 ggggatcgaa gtggttaaca ccaccgccga ggccgttatg
cttgcccttg ccgcgaccct 8760 cacgctggcg ctcgtggtag ttgtggccgg
taacattggt gatgaggtag ttgggccagc 8820 cnacgannnn ctcagtaaga
tgagcgagct cgtgggtcat ctttccgaga cgagtagcct 8880 gctgctcgcg
ggttcgggga acgaagacca tgtcacgctc catgttgcca gtggccttgt 8940
ggtgctttcg gtgggagatt tgccagctga agtaggggac aaggagggaa gagtgaagaa
9000 cccagccagt aatgtcgttg atgatgcgag aatcggagaa agcaccgtga
ccgcactcat 9060 gggcaataac ccagagacca gtaccgaaaa gaccctgaag
aacggtgtac acggcccaca 9120 gaccagcgcg ggcgggggtg gaggggatat
attcgggggt cacaaagttg taccagatgc 9180 tgaaagtggt agtcaggagg
acaatgtcgc ggaggatata accgtatccc ttgagagcgg 9240 agcgcttgaa
gcagtgctta gggatggcat tgtagatgtc cttgatggta aagtcgggaa 9300
cctcgaactg gttgccgtag gtgtcgagca tgacaccata ctcggacttg ggcttggcga
9360 tatcaacctc ggacatggac gagagcgatg tggaagaggc cgagtggcgg
ggagagtctg 9420 aaggagagac ggcggcagac tcagaatccg tcacagtagt
tgaggtgacg gtgcgtctaa 9480 gcgcagggtt ctgcttgggc agagccgaag
tggacgccat ggttgatgtg tgtttaattc 9540 aagaatgaat atagagaaga
gaagaagaaa aaagattcaa ttgagccggc gatgcagacc 9600 cttatataaa
tgttgccttg gacagacgga gcaagcccgc ccaaacctac gttcggtata 9660
atatgttaag ctttttaaca caaaggtttg gcttggggta acctgatgtg gtgcaaaaga
9720 ccgggcgttg gcgagccatt gcgcgggcga atggggccgt gactcgtctc
aaattcgagg 9780 gcgtgcctca attcgtgccc ccgtggcttt ttcccgccgt
ttccgccccg tttgcaccac 9840 tgcagccgct tctttggttc ggacaccttg
ctgcgagcta ggtgccttgt gctacttaaa 9900 aagtggcctc ccaacaccaa
catgacatga gtgcgtgggc caagacacgt tggcggggtc 9960 gcagtcggct
caatggcccg gaaaaaacgc tgctggagct ggttcggacg cagtccgccg 10020
cggcgtatgg atatccgcaa ggttccatag cgccattgcc ctccgtcggc gtctatcccg
10080 caacctctaa atagagcggg aatataaccc aagcttcttt tttttccttt
aacacgcaca 10140 cccccaacta tcatgttgct gctgctgttt gactctactc
tgtggagggg tgctcccacc 10200 caacccaacc tacaggtgga tccggcgctg
tgattggctg ataagtctcc tatccggact 10260 aattctgacc aatgggacat
gcgcgcagga cccaaatgcc gcaattacgt aaccccaacg 10320 aaatgcctac
ccctctttgg agcccagcgg ccccaaatcc ccccaagcag cccggttcta 10380
ccggcttcca tctccaagca caagcagccc ggttctaccg gcttccatct ccaagcaccc
10440 ctttctccac accccacaaa aagacccgtg caggacatcc tactgcgtcg
acatcattta 10500 aattccttca cttcaagttc attcttcatc tgcttctgtt
ttactttgac aggcaaatga 10560 agacatggta cgacttgatg gaggccaaga
acgccatttc accccgagac accgaagtgc 10620 ctgaaatcct ggctgccccc
attgataaca tcggaaacta cggtattccg gaaagtgtat 10680
atagaacctt tccccagctt gtgtctgtgg atatggatgg tgtaatcccc tttgagtact
10740 cgtcttggct tctctccgag cagtatgagg ctctctaatc tagcgcattt
aatatctcaa 10800 tgtatttata tatttatctt ctcatgcggc cgctcactga
atctttttgg ctcccttgtg 10860 cttcctgacg atatacgttt gcacatagaa
attcaagaac aaacacaaga ctgtgccaac 10920 ataaaagtaa ttgaagaacc
agccaaacat cctcatccca tcttggcgat aacagggaat 10980 gttcctgtac
ttccagacaa tgtagaaacc aacattgaat tgaatgatct gcattgatgt 11040
aatcagggat tttggcatgg ggaacttcag cttgatcaat ctggtccaat aataaccgta
11100 catgatccag tggatgaaac cattcaacag cacaaaaatc caaacagctt
catttcggta 11160 attatagaac agccacatat ccatcggtgc ccccaaatga
tggaagaatt gcaaccaggt 11220 cagaggcttg cccatcagtg gcaaatagaa
ggagtcaata tactccagga acttgctcaa 11280 atagaacaac tgcgtggtga
tcctgaagac gttgttgtca aaagccttct cgcagttgtc 11340 agacataaca
ccgatggtgt acatggcata tgccattgag aggaatgatc ccaacgaata 11400
aatggacatg agaaggttgt aattggtgaa aacaaacttc atacgagact gaccttttgg
11460 accaaggggg ccaagagtga acttcaagat gacaaatgcg atggacaagt
aaagcacctc 11520 acagtgactg gcatcactcc agagttgggc ataatcaact
ggttgggtaa aacttcctgc 11580 ccaattgaga ctatttcatt caccacctcc
atggccattg ctgtagatat gtcttgtgtg 11640 taagggggtt ggggtggttg
tttgtgttct tgacttttgt gttagcaagg gaagacgggc 11700 aaaaaagtga
gtgtggttgg gagggagaga cgagccttat atataatgct tgtttgtgtt 11760
tgtgcaagtg gacgccgaaa cgggcaggag ccaaactaaa caaggcagac aatgcgagct
11820 taattggatt gcctgatggg caggggttag ggctcgatca atgggggtgc
gaagtgacaa 11880 aattgggaat taggttcgca agcaaggctg acaagacttt
ggcccaaaca tttgtacgcg 11940 gtggacaaca ggagccaccc atcgtctgtc
acgggctagc cggtcgtgcg tcctgtcagg 12000 ctccacctag gctccatgcc
actccataca atcccactag tgtaccgcta ggccgctttt 12060 agctcccatc
taagaccccc ccaaaacctc cactgtacag tgcactgtac tgtgtggcga 12120
tcaagggcaa gggaaaaaag gcgcaaacat gcacgcatgg aatgacgtag gtaaggcgtt
12180 actagactga aaagtggcac atttcggcgt gccaaagggt cctaggtgcg
tttcgcgagc 12240 tgggcgccag gccaagccgc tccaaaacgc ctctccgact
ccctccagcg gcctccatat 12300 ccccatccct ctccacagca atgttgttaa
gccttgcaaa cgaaaaaata gaaaggctaa 12360 taagcttcca atattgtggt
gtacgctgca taacgcaaca atgagcgcca aacaacacac 12420 acacacagca
cacagcagca ttaaccacga tgaacagcat gacattacag gtgggtgtgt 12480
aatcagggcc ctgattgctg gtggtgggag cccccatcat gggcagatct gcgtacactg
12540 tttaaacagt gtacgcagat ctactataga ggaacattta aattgccccg
gagaagacgg 12600 ccaggccgcc tagatgacaa attcaacaac tcacagctga
ctttctgcca ttgccactag 12660 gggggggcct ttttatatgg ccaagccaag
ctctccacgt cggttgggct gcacccaaca 12720 ataaatgggt agggttgcac
caacaaaggg atgggatggg gggtagaaga tacgaggata 12780 acggggctca
atggcacaaa taagaacgaa tactgccatt aagactcgtg atccagcgac 12840
tgacaccatt gcatcatcta agggcctcaa aactacctcg gaactgctgc gctgatctgg
12900 acaccacaga ggttccgagc actttaggtt gcaccaaatg tcccaccagg
tgcaggcaga 12960 aaacgctgga acagcgtgta cagtttgtct taacaaaaag
tgagggcgct gaggtcgagc 13020 agggtggtgt gacttgttat agcctttaga
gctgcgaaag cgcgtatgga tttggctcat 13080 caggccagat tgagggtctg
tggacacatg tcatgttagt gtacttcaat cgccccctgg 13140 atatagcccc
gacaataggc cgtggcctca tttttttgcc ttccgcacat ttccattgct 13200
cgatacccac accttgcttc tcctgcactt gccaacctta atactggttt acattgacca
13260 acatcttaca agcggggggc ttgtctaggg tatatataaa cagtggctct
cccaatcggt 13320 tgccagtctc ttttttcctt tctttcccca cagattcgaa
atctaaacta cacatcacag 13380 aattccgagc cgtgagtatc cacgacaaga
tcagtgtcga gacgacgcgt tttgtgtaat 13440 gacacaatcc gaaagtcgct
agcaacacac actctctaca caaactaacc cagctctggt 13500 accatggagg
tcgtgaacga aatcgtctcc attggccagg aggttcttcc caaggtcgac 13560
tatgctcagc tctggtctga tgcctcgcac tgcgaggtgc tgtacctctc catcgccttc
13620 gtcatcctga agttcaccct tggtcctctc ggacccaagg gtcagtctcg
aatgaagttt 13680 gtgttcacca actacaacct gctcatgtcc atctactcgc
tgggctcctt cctctctatg 13740 gcctacgcca tgtacaccat tggtgtcatg
tccgacaact gcgagaaggc tttcgacaac 13800 aatgtcttcc gaatcaccac
tcagctgttc tacctcagca agttcctcga gtacattgac 13860 tccttctatc
tgcccctcat gggcaagcct ctgacctggt tgcagttctt tcaccatctc 13920
ggagctccta tggacatgtg gctgttctac aactaccgaa acgaagccgt ttggatcttt
13980 gtgctgctca acggcttcat tcactggatc atgtacggct actattggac
ccgactgatc 14040 aagctcaagt tccctatgcc caagtccctg attacttcta
tgcagatcat tcagttcaac 14100 gttggcttct acatcgtctg gaagtaccgg
aacattccct gctaccgaca agatggaatg 14160 agaatgtttg gctggttttt
caactacttc tacgttggta ctgtcctgtg tctgttcctc 14220 aacttctacg
tgcagaccta catcgtccga aagcacaagg gagccaaaaa gattcagtga 14280
gcggccgcat gtacatacaa gattatttat agaaatgaat cgcgatcgaa caaagagtac
14340 gagtgtacga gtaggggatg atgataaaag tggaagaagt tccgcatctt
tggatttatc 14400 aacgtgtagg acgatacttc ctgtaaaaat gcaatgtctt
taccataggt tctgctgtag 14460 atgttattaa ctaccattaa catgtctact
tgtacagttg cagaccagtt ggagtataga 14520 atggtacact taccaaaaag
tgttgatggt tgtaactacg atatataaaa ctgttgacgg 14580 gatccccgct
gatatgccta aggaacaatc aaagaggaag atattaattc agaatgctag 14640
tatacagtta gggat 14655 <210> SEQ ID NO 64 <211> LENGTH:
777 <212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <223>
OTHER INFORMATION: synthetic delta-9 elongase (codon-optimized)
<300> PUBLICATION INFORMATION: <302> TITLE: DELTA-9
ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310> PATENT DOCUMENT NUMBER: WO 2007/061742 <311>
PATENT FILING DATE: 2006-11-16 <312> PUBLICATION DATE:
2007-05-31 <313> RELEVANT RESIDUES IN SEQ ID NO: (1)..(777)
<400> SEQUENCE: 64 atggaggtcg tgaacgaaat cgtctccatt
ggccaggagg ttcttcccaa ggtcgactat 60 gctcagctct ggtctgatgc
ctcgcactgc gaggtgctgt acctctccat cgccttcgtc 120 atcctgaagt
tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg 180
ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc
240 tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt
cgacaacaat 300 gtcttccgaa tcaccactca gctgttctac ctcagcaagt
tcctcgagta cattgactcc 360 ttctatctgc ccctcatggg caagcctctg
acctggttgc agttctttca ccatctcgga 420 gctcctatgg acatgtggct
gttctacaac taccgaaacg aagccgtttg gatctttgtg 480 ctgctcaacg
gcttcattca ctggatcatg tacggctact attggacccg actgatcaag 540
ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt
600 ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga
tggaatgaga 660 atgtttggct ggtttttcaa ctacttctac gttggtactg
tcctgtgtct gttcctcaac 720 ttctacgtgc agacctacat cgtccgaaag
cacaagggag ccaaaaagat tcagtga 777 <210> SEQ ID NO 65
<211> LENGTH: 258 <212> TYPE: PRT <213> ORGANISM:
Euglena gracilis <220> FEATURE: <221> NAME/KEY:
MISC_FEATURE <222> LOCATION: (1)..(258) <223> OTHER
INFORMATION: delta-9 elongase (EgD9e) <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-9 ELONGASES AND THEIR USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: WO 2007/061742 <311> PATENT FILING DATE: 2006-11-16
<312> PUBLICATION DATE: 2007-05-31 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(258) <400> SEQUENCE: 65 Met Glu
Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 1 5 10 15
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 20
25 30 Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly
Pro 35 40 45 Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe
Thr Asn Tyr 50 55 60 Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser
Phe Leu Ser Met Ala 65 70 75 80 Tyr Ala Met Tyr Thr Ile Gly Val Met
Ser Asp Asn Cys Glu Lys Ala 85 90 95 Phe Asp Asn Asn Val Phe Arg
Ile Thr Thr Gln Leu Phe Tyr Leu Ser 100 105 110 Lys Phe Leu Glu Tyr
Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 115 120 125 Pro Leu Thr
Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 130 135 140 Met
Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 145 150
155 160 Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp
Thr 165 170 175 Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu
Ile Thr Ser 180 185 190 Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr
Ile Val Trp Lys Tyr 195 200 205 Arg Asn Ile Pro Cys Tyr Arg Gln Asp
Gly Met Arg Met Phe Gly Trp 210 215 220 Phe Phe Asn Tyr Phe Tyr Val
Gly Thr Val Leu Cys Leu Phe Leu Asn 225 230 235 240 Phe Tyr Val Gln
Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 245 250 255 Ile
Gln
<210> SEQ ID NO 66 <211> LENGTH: 34 <212> TYPE:
DNA <213> ORGANISM: Escherichia coli <400> SEQUENCE: 66
ataacttcgt ataatgtatg ctatacgaag ttat 34 <210> SEQ ID NO 67
<211> LENGTH: 828 <212> TYPE: DNA <213> ORGANISM:
Mortierella alpina <220> FEATURE: <221> NAME/KEY: CDS
<222> LOCATION: (1)..(828) <223> OTHER INFORMATION:
synthetic C16/18 elongase (codon-optimized) <300> PUBLICATION
INFORMATION: <302> TITLE: A MORTIERELLA ALPINA C16/18 FATTY
ACID ELONGASE <310> PATENT DOCUMENT NUMBER: US
2007/0087420-A1 <311> PATENT FILING DATE: 2005-10-19
<312> PUBLICATION DATE: 2007-04-19 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(828) <400> SEQUENCE: 67 atg gag
tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat 48 Met Glu
Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 1 5 10 15
gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac 96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 20
25 30 act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc
cga 144 Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser
Arg 35 40 45 gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga
acc cag tcc 192 Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg
Thr Gln Ser 50 55 60 gga gct gcc atg act gcc ttc gtc ttt gtg cac
aac ctc att ctg tgt 240 Gly Ala Ala Met Thr Ala Phe Val Phe Val His
Asn Leu Ile Leu Cys 65 70 75 80 gtc tac tct ggc atc acc ttc tac tac
atg ttt cct gct atg gtc aag 288 Val Tyr Ser Gly Ile Thr Phe Tyr Tyr
Met Phe Pro Ala Met Val Lys 85 90 95 aac ttc cga acc cac aca ctg
cac gaa gcc tac tgc gac acg gat cag 336 Asn Phe Arg Thr His Thr Leu
His Glu Ala Tyr Cys Asp Thr Asp Gln 100 105 110 tcc ctc tgg aac aac
gca ctt ggc tac tgg ggt tac ctc ttc tac ctg 384 Ser Leu Trp Asn Asn
Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 115 120 125 tcc aag ttc
tac gag gtc att gac acc atc atc atc atc ctg aag gga 432 Ser Lys Phe
Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 130 135 140 cga
cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att 480 Arg
Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 145 150
155 160 acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc
ttt 528 Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile
Phe 165 170 175 gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt
tac tat gcc 576 Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys
Tyr Tyr Ala 180 185 190 ttc acc tct atc gga ttc cat cct cct ggc aaa
aag tac ctg act tcg 624 Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys
Lys Tyr Leu Thr Ser 195 200 205 atg cag att act cag ttt ctg gtc ggt
atc acc att gcc gtg tcc tac 672 Met Gln Ile Thr Gln Phe Leu Val Gly
Ile Thr Ile Ala Val Ser Tyr 210 215 220 ctc ttc gtt cct ggc tgc atc
cga aca ccc ggt gct cag atg gct gtc 720 Leu Phe Val Pro Gly Cys Ile
Arg Thr Pro Gly Ala Gln Met Ala Val 225 230 235 240 tgg atc aac gtc
ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac 768 Trp Ile Asn Val
Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 245 250 255 ttt gcc
aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa 816 Phe Ala
Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 260 265 270
aag gct cag taa 828 Lys Ala Gln 275 <210> SEQ ID NO 68
<211> LENGTH: 275 <212> TYPE: PRT <213> ORGANISM:
Mortierella alpina <400> SEQUENCE: 68 Met Glu Ser Gly Pro Met
Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 1 5 10 15 Asp Phe Phe Met
Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 20 25 30 Thr Ala
Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 35 40 45
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 50
55 60 Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu
Cys 65 70 75 80 Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala
Met Val Lys 85 90 95 Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr
Cys Asp Thr Asp Gln 100 105 110 Ser Leu Trp Asn Asn Ala Leu Gly Tyr
Trp Gly Tyr Leu Phe Tyr Leu 115 120 125 Ser Lys Phe Tyr Glu Val Ile
Asp Thr Ile Ile Ile Ile Leu Lys Gly 130 135 140 Arg Arg Ser Ser Leu
Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 145 150 155 160 Thr Met
Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 165 170 175
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 180
185 190 Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr
Ser 195 200 205 Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala
Val Ser Tyr 210 215 220 Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly
Ala Gln Met Ala Val 225 230 235 240 Trp Ile Asn Val Gly Tyr Leu Phe
Pro Leu Thr Tyr Leu Phe Val Asp 245 250 255 Phe Ala Lys Arg Thr Tyr
Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 260 265 270 Lys Ala Gln 275
<210> SEQ ID NO 69 <211> LENGTH: 8739 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY116 <400> SEQUENCE:
69 ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc
gggtggacgt 60 ctagaggtac ctagcaatta acagatagtt tgccggtgat
aattctctta acctcccaca 120 ctcctttgac ataacgattt atgtaacgaa
actgaaattt gaccagatat tgtgtccgcg 180 gtggagctcc agcttttgtt
ccctttagtg agggtttaaa cgagcttggc gtaatcatgg 240 tcatagctgt
ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cgtacgagcc 300
ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
360 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
ttaatgaatc 420 ggccaacgcg cggggagagg cggtttgcgt attgggcgct
cttccgcttc ctcgctcact 480 gactcgctgc gctcggtcgt tcggctgcgg
cgagcggtat cagctcactc aaaggcggta 540 atacggttat ccacagaatc
aggggataac gcaggaaaga acatgtgagc aaaaggccag 600 caaaaggcca
ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 660
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
720 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
tccgaccctg 780 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
gcgtggcgct ttctcatagc 840 tcacgctgta ggtatctcag ttcggtgtag
gtcgttcgct ccaagctggg ctgtgtgcac 900 gaaccccccg ttcagcccga
ccgctgcgcc ttatccggta actatcgtct tgagtccaac 960 ccggtaagac
acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1020
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
1080 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
aagagttggt 1140 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
gtttttttgt ttgcaagcag 1200 cagattacgc gcagaaaaaa aggatctcaa
gaagatcctt tgatcttttc tacggggtct 1260 gacgctcagt ggaacgaaaa
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 1320 atcttcacct
agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1380
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
1440 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
tacgatacgg 1500 gagggcttac catctggccc cagtgctgca atgataccgc
gagacccacg ctcaccggct 1560 ccagatttat cagcaataaa ccagccagcc
ggaagggccg agcgcagaag tggtcctgca 1620 actttatccg cctccatcca
gtctattaat tgttgccggg aagctagagt aagtagttcg 1680 ccagttaata
gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1740
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
1800 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
cagaagtaag 1860 ttggccgcag tgttatcact catggttatg gcagcactgc
ataattctct tactgtcatg 1920 ccatccgtaa gatgcttttc tgtgactggt
gagtactcaa ccaagtcatt ctgagaatag 1980
tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
2040 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
actctcaagg 2100 atcttaccgc tgttgagatc cagttcgatg taacccactc
gtgcacccaa ctgatcttca 2160 gcatctttta ctttcaccag cgtttctggg
tgagcaaaaa caggaaggca aaatgccgca 2220 aaaaagggaa taagggcgac
acggaaatgt tgaatactca tactcttcct ttttcaatat 2280 tattgaagca
tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 2340
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
2400 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
cgctacactt 2460 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
cctttctcgc cacgttcgcc 2520 ggctttcccc gtcaagctct aaatcggggg
ctccctttag ggttccgatt tagtgcttta 2580 cggcacctcg accccaaaaa
acttgattag ggtgatggtt cacgtagtgg gccatcgccc 2640 tgatagacgg
tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 2700
ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
2760 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
taacgcgaat 2820 tttaacaaaa tattaacgct tacaatttcc attcgccatt
caggctgcgc aactgttggg 2880 aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg 2940 caaggcgatt aagttgggta
acgccagggt tttcccagtc acgacgttgt aaaacgacgg 3000 ccagtgaatt
gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg 3060
tcgatggtgt cgataagctt gatatcgaat tcatgtcaca caaaccgatc ttcgcctcaa
3120 ggaaacctaa ttctacatcc gagagactgc cgagatccag tctacactga
ttaattttcg 3180 ggccaataat ttaaaaaaat cgtgttatat aatattatat
gtattatata tatacatcat 3240 gatgatactg acagtcatgt cccattgcta
aatagacaga ctccatctgc cgcctccaac 3300 tgatgttctc aatatttaag
gggtcatctc gcattgttta ataataaaca gactccatct 3360 accgcctcca
aatgatgttc tcaaaatata ttgtatgaac ttatttttat tacttagtat 3420
tattagacaa cttacttgct ttatgaaaaa cacttcctat ttaggaaaca atttataatg
3480 gcagttcgtt catttaacaa tttatgtaga ataaatgtta taaatgcgta
tgggaaatct 3540 taaatatgga tagcataaat gatatctgca ttgcctaatt
cgaaatcaac agcaacgaaa 3600 aaaatccctt gtacaacata aatagtcatc
gagaaatatc aactatcaaa gaacagctat 3660 tcacacgtta ctattgagat
tattattgga cgagaatcac acactcaact gtctttctct 3720 cttctagaaa
tacaggtaca agtatgtact attctcattg ttcatacttc tagtcatttc 3780
atcccacata ttccttggat ttctctccaa tgaatgacat tctatcttgc aaattcaaca
3840 attataataa gatataccaa agtagcggta tagtggcaat caaaaagctt
ctctggtgtg 3900 cttctcgtat ttatttttat tctaatgatc cattaaaggt
atatatttat ttcttgttat 3960 ataatccttt tgtttattac atgggctgga
tacataaagg tattttgatt taattttttg 4020 cttaaattca atcccccctc
gttcagtgtc aactgtaatg gtaggaaatt accatacttt 4080 tgaagaagca
aaaaaaatga aagaaaaaaa aaatcgtatt tccaggttag acgttccgca 4140
gaatctagaa tgcggtatgc ggtacattgt tcttcgaacg taaaagttgc gctccctgag
4200 atattgtaca tttttgcttt tacaagtaca agtacatcgt acaactatgt
actactgttg 4260 atgcatccac aacagtttgt tttgtttttt tttgtttttt
ttttttctaa tgattcatta 4320 ccgctatgta tacctacttg tacttgtagt
aagccgggtt attggcgttc aattaatcat 4380 agacttatga atctgcacgg
tgtgcgctgc gagttacttt tagcttatgc atgctacttg 4440 ggtgtaatat
tgggatctgt tcggaaatca acggatgctc aaccgatttc gacagtaatt 4500
aattaatttg aatcgaatcg gagcctaaaa tgaacccgag tatatctcat aaaattctcg
4560 gtgagaggtc tgtgactgtc agtacaaggt gccttcatta tgccctcaac
cttaccatac 4620 ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg
ccaaaagcca aggcactgag 4680 ctcgtctaac ggacttgata tacaaccaat
taaaacaaat gaaaagaaat acagttcttt 4740 gtatcatttg taacaattac
cctgtacaaa ctaaggtatt gaaatcccac aatattccca 4800 aagtccaccc
ctttccaaat tgtcatgcct acaactcata taccaagcac taacctacca 4860
aacaccacta aaaccccaca aaatatatct taccgaatat acagtaacaa gctaccacca
4920 cactcgttgg gtgcagtcgc cagcttaaag atatctatcc acatcagcca
caactccctt 4980 cctttaataa accgactaca cccttggcta ttgaggttat
gagtgaatat actgtagaca 5040 agacactttc aagaagactg tttccaaaac
gtaccactgt cctccactac aaacacaccc 5100 aatctgcttc ttctagtcaa
ggttgctaca ccggtaaatt ataaatcatc atttcattag 5160 cagggcaggg
ccctttttat agagtcttat acactagcgg accctgccgg tagaccaacc 5220
cgcaggcgcg tcagtttgct ccttccatca atgcgtcgta gaaacgactt actccttctt
5280 gagcagctcc ttgaccttgt tggcaacaag tctccgacct cggaggtgga
ggaagagcct 5340 ccgatatcgg cggtagtgat accagcctcg acggactcct
tgacggcagc ctcaacagcg 5400 tcaccggcgg gcttcatgtt aagagagaac
ttgagcatca tggcggcaga cagaatggtg 5460 gcaatggggt tgaccttctg
cttgccgaga tcgggggcag atccgtgaca gggctcgtac 5520 agaccgaacg
cctcgttggt gtcgggcaga gaagccagag aggcggaggg cagcagaccc 5580
agagaaccgg ggatgacgga ggcctcgtcg gagatgatat cgccaaacat gttggtggtg
5640 atgatgatac cattcatctt ggagggctgc ttgatgagga tcatggcggc
cgagtcgatc 5700 agctggtggt tgagctcgag ctgggggaat tcgtccttga
ggactcgagt gacagtcttt 5760 cgccaaagtc gagaggaggc cagcacgttg
gccttgtcaa gagaccacac gggaagaggg 5820 gggttgtgct gaagggccag
gaaggcggcc attcgggcaa ttcgctcaac ctcaggaacg 5880 gagtaggtct
cggtgtcgga agcgacgcca gatccgtcat cctcctttcg ctctccaaag 5940
tagatacctc cgacgagctc tcggacaatg atgaagtcgg tgccctcaac gtttcggatg
6000 ggggagagat cggcgagctt gggcgacagc agctggcagg gtcgcaggtt
ggcgtacagg 6060 ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc
gcacgtcggt tcgtccgtcg 6120 ggagtggtcc atacggtgtt ggcagcgcct
ccgacagcac cgagcataat agagtcagcc 6180 tttcggcaga tgtcgagagt
agcgtcggtg atgggctcgc cctccttctc aatggcagct 6240 cctccaatga
gtcggtcctc aaacacaaac tcggtgccgg aggcctcagc aacagacttg 6300
agcaccttga cggcctcggc aatcacctcg gggccacaga agtcgccgcc gagaagaaca
6360 atcttcttgg agtcagtctt ggtcttctta gtttcgggtt ccattgtgga
tgtgtgtggt 6420 tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct
ggcaaacgct cttgtatata 6480 tacgcacttt tgcccgtgct atgtggaaga
ctaaacctcc gaagattgtg actcaggtag 6540 tgcggtatcg gctagggacc
caaaccttgt cgatgccgat agcgctatcg aacgtacccc 6600 agccggccgg
gagtatgtcg gaggggacat acgagatcgt caagggtttg tggccaactg 6660
gtatttaaat gtagctaacg gtagcaggcg aactactggt acatacctcc cccggaatat
6720 gtacaggcat aatgcgtatc tgtgggacat gtggtcgttg cgccattatg
taagcagcgt 6780 gtactcctct gactgtccat atggtttgct ccatctcacc
ctcatcgttt tcattgttca 6840 caggcggcca caaaaaaact gtcttctctc
cttctctctt cgccttagtc tactcggacc 6900 agttttagtt tagcttggcg
ccactggata aatgagacct caggccttgt gatgaggagg 6960 tcacttatga
agcatgttag gaggtgcttg tatggataga gaagcaccca aaataataag 7020
aataataata aaacaggggg cgttgtcatt tcatatcgtg ttttcaccat caatacacct
7080 ccaaacaatg cccttcatgt ggccagcccc aatattgtcc tgtagttcaa
ctctatgcag 7140 ctcgtatctt attgagcaag taaaactctg tcagccgata
ttgcccgacc cgcgacaagg 7200 gtcaacaagg tggtgtaagg ccttcgcaga
agtcaaaact gtgccaaaca aacatctaga 7260 gtctctttgg tgtttctcgc
atatatttwa tcggctgtct tacgtatttg cgcctcggta 7320 ccggactaat
ttcggatcat ccccaatacg ctttttcttc gcagctgtca acagtgtcca 7380
tgatctatcc acctaaatgg gtcatatgag gcgtataatt tcgtggtgct gataataatt
7440 cccatatatt tgacacaaaa cttccccccc tagacataca tctcacaatc
tcacttcttg 7500 tgcttctgtc acacatctcc tccagctgac ttcaactcac
acctctgccc cagttggtct 7560 acagcggtat aaggtttctc cgcatagagg
tgcaccactc ctcccgatac ttgtttgtgt 7620 gacttgtggg tcacgacata
tatatctaca cacattgcgc caccctttgg ttcttccagc 7680 acaacaaaaa
cacgacacgc taaccatggc caatttactg accgtacacc aaaatttgcc 7740
tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag
7800 ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
gccggtcgtg 7860 ggcggcatgg tgcaagttga ataaccggaa atggtttccc
gcagaacctg aagatgttcg 7920 cgattatctt ctatatcttc aggcgcgcgg
tctggcagta aaaactatcc agcaacattt 7980 gggccagcta aacatgcttc
atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc 8040 tgtttcactg
gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa 8100
acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag
8160 cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
acaccctgtt 8220 acgtatagcc gaaattgcca ggatcagggt taaagatatc
tcacgtactg acggtgggag 8280 aatgttaatc catattggca gaacgaaaac
gctggttagc accgcaggtg tagagaaggc 8340 acttagcctg ggggtaacta
aactggtcga gcgatggatt tccgtctctg gtgtagctga 8400 tgatccgaat
aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc 8460
caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat
8520 ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
acagtgcccg 8580 tgtcggagcc gcgcgagata tggcccgcgc tggagtttca
ataccggaga tcatgcaagc 8640 tggtggctgg accaatgtaa atattgtcat
gaactatatc cgtaacctgg atagtgaaac 8700 aggggcaatg gtgcgcctgc
tggaagatgg cgattaagc 8739 <210> SEQ ID NO 70 <211>
LENGTH: 15304 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Plasmid pKO2UF8289 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (5601)..(5601)
<223> OTHER INFORMATION: n is a, c, g, or t <220>
FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:
(5606)..(5609) <223> OTHER INFORMATION: n is a, c, g, or t
<400> SEQUENCE: 70 cgatcgagga agaggacaag cggctgcttc
ttaagtttgt gacatcagta tccaaggcac 60 cattgcaagg attcaaggct
ttgaacccgt catttgccat tcgtaacgct ggtagacagg 120 ttgatcggtt
ccctacggcc tccacctgtg tcaatcttct caagctgcct gactatcagg 180
acattgatca acttcggaag aaacttttgt atgccattcg atcacatgct ggtttcgatt
240
tgtcttagag gaacgcatat acagtaatca tagagaataa acgatattca tttattaaag
300 tagatagttg aggtagaagt tgtaaagagt gataaatagc ggccgctcac
tgaatctttt 360 tggctccctt gtgcttcctg acgatatacg tttgcacata
gaaattcaag aacaaacaca 420 agactgtgcc aacataaaag taattgaaga
accagccaaa catcctcatc ccatcttggc 480 gataacaggg aatgttcctg
tacttccaga caatgtagaa accaacattg aattgaatga 540 tctgcattga
tgtaatcagg gattttggca tggggaactt cagcttgatc aatctggtcc 600
aataataacc gtacatgatc cagtggatga aaccattcaa cagcacaaaa atccaaacag
660 cttcatttcg gtaattatag aacagccaca tatccatcgg tgcccccaaa
tgatggaaga 720 attgcaacca ggtcagaggc ttgcccatca gtggcaaata
gaaggagtca atatactcca 780 ggaacttgct caaatagaac aactgcgtgg
tgatcctgaa gacgttgttg tcaaaagcct 840 tctcgcagtt gtcagacata
acaccgatgg tgtacatggc atatgccatt gagaggaatg 900 atcccaacga
ataaatggac atgagaaggt tgtaattggt gaaaacaaac ttcatacgag 960
actgaccttt tggaccaagg gggccaagag tgaacttcaa gatgacaaat gcgatggaca
1020 agtaaagcac ctcacagtga ctggcatcac tccagagttg ggcataatca
actggttggg 1080 taaaacttcc tgcccaattg agactatttc attcaccacc
tccatggtta gcgtgtcgtg 1140 tttttgttgt gctggaagaa ccaaagggtg
gcgcaatgtg tgtagatata tatgtcgtga 1200 cccacaagtc acacaaacaa
gtatcgggag gagtggtgca cctctatgcg gagaaacctt 1260 ataccgctgt
agaccaactg gggcagaggt gtgagttgaa gtcagctgga ggagatgtgt 1320
gacagaagca caagaagtga gattgtgaga tgtatgtcta gggggggaag ttttgtgtca
1380 aatatatggg aattattatc agcaccacga aattatacgc ctcatatgac
ccatttaggt 1440 ggatagatca tggacactgt tgacagctgc gaagaaaaag
cgtattgggg atgatccgaa 1500 attagtccgg taccgaggcg caaatacgta
agacagccga twaaatatat gcgagaaaca 1560 ccaaagagac tctagatgtt
tgtttggcac agttttgact tctgcgaagg ccttacacca 1620 ccttgttgac
ccttgtcgcg ggtcgggcaa tatcggctga cagagtttta cttgctcaat 1680
aagatacgag ctgcatagag ttgaactaca ggacaatatt ggggctggcc acatgaaggg
1740 cattgtttgg aggtgtattg atggtgaaaa cacgatatga aatgacaacg
ccccctgttt 1800 tattattatt cttattattt tgggtgcttc tctatccata
caagcacctc ctaacatgct 1860 tcataagtga cctcctcatc acaaggcctg
aggtctcatt tatccagtgg cgccaagcta 1920 aactaaaact ggtccgagta
gactaaggcg aagagagaag gagagaagac agtttttttg 1980 tggccgcctg
tgaacaatga aaacgatgag ggtgagatgg agcaaaccat atggtttaaa 2040
cagtcagagg agtacacgct gcttacataa tggcgcaacg accacatgtc ccacagatac
2100 gcatcgattc gattcaaatt aattaaaagg cgttgaaaca gaatgagcca
gacagcaagg 2160 acaaggtggc caacagcaag gagtccaaaa agccctctat
tgacgagatc cacgatgtta 2220 ttgctcatga ggtttccgag ctcgatgctg
ggaagaagaa gtgatttgta tataagaaat 2280 aaatgagata tagtaaagga
gtgcaagaga atggcaaggt ggtcaaattc tatattactt 2340 gcagtcactg
gttcctcgtt gacatgaatg aagttaccgt tggcatagct gatttaatat 2400
ataactgtcc aactaactct cacctagata taacccatgt gtgtgtttcc aatcatcaat
2460 gcggccgctt actgagcctt ggcaccgggc tgcttctcgg ccattcgagc
gaactgggac 2520 aggtatcgga gcaggatgac gagaccttca tggggcagag
ggtttcggta ggggaggttg 2580 tgcttctggc acagctgttc cacctggtag
gaaacggcag tgaggttgtg tcgaggcagg 2640 gtgggccaga gatggtgctc
gatctggtag ttcaggcctc caaagaacca gtcagtaatg 2700 atgcctcgtc
gaatgttcat ggtctcatgg atctgaccca cagagaagcc atgtccgtcc 2760
cagacggaat caccgatctt ctccagaggg tagtggttca tgaagaccac gatggcaatt
2820 ccgaagccac cgacgagctc ggaaacaaag aacaccagca tcgaggtcag
gatggagggc 2880 ataaagaaga ggtggaacag ggtcttgaga gtccagtgca
gagcgagtcc aatggcctct 2940 ttcttgtact gagatcggta gaactggttg
tctcggtcct tgagggatcg aacggtcagc 3000 acagactgga aacaccagat
gaatcgcagg agaatacaga tgaccaggaa atagtactgt 3060 tggaactgaa
tgagctttcg ggagatggga gaagctcgag tgacatcgtc ctcggaccag 3120
gcgagcagag gcaggttatc aatgtcggga tcgtgaccct gaacgttggt agcagaatga
3180 tgggcgttgt gtctgtcctt ccaccaggtc acggagaagc cctggagtcc
gttgccaaag 3240 accagaccca ggacgttatt ccagtttcgg ttcttgaagg
tctggtggtg gcagatgtca 3300 tgagacagcc atcccatttg ctggtagtgc
ataccgagca cgagagcacc aatgaagtac 3360 aggtggtact ggaccagcat
gaagaaggca agcacgccaa gacccagggt ggtcaagatc 3420 ttgtacgagt
accagagggg agaggcgtca aacatgccag tggcgatcag ctcttctcgg 3480
agctttcgga aatcctcctg agcttcgttg acggcagcct ggggaggcag ctcggaagcc
3540 tggttgatct tgggcattcg cttgagcttg tcgaaggctt cctgagagtg
cataaccatg 3600 aaggcgtcag tagcatctcg tccctggtag ttctcaatga
tttcagctcc accagggtgg 3660 aagttcaccc aagcggagac gtcgtacacc
tttccgtcga tgacgagggg cagagcctgt 3720 cgagaagcct tcaccatggc
cattgctgta gatatgtctt gtgtgtaagg gggttggggt 3780 ggttgtttgt
gttcttgact tttgtgttag caagggaaga cgggcaaaaa agtgagtgtg 3840
gttgggaggg agagacgagc cttatatata atgcttgttt gtgtttgtgc aagtggacgc
3900 cgaaacgggc aggagccaaa ctaaacaagg cagacaatgc gagcttaatt
ggattgcctg 3960 atgggcaggg gttagggctc gatcaatggg ggtgcgaagt
gacaaaattg ggaattaggt 4020 tcgcaagcaa ggctgacaag actttggccc
aaacatttgt acgcggtgga caacaggagc 4080 cacccatcgt ctgtcacggg
ctagccggtc gtgcgtcctg tcaggctcca cctaggctcc 4140 atgccactcc
atacaatccc actagtgtac cgctaggccg cttttagctc ccatctaaga 4200
cccccccaaa acctccactg tacagtgcac tgtactgtgt ggcgatcaag ggcaagggaa
4260 aaaaggcgca aacatgcacg catggaatga cgtaggtaag gcgttactag
actgaaaagt 4320 ggcacatttc ggcgtgccaa agggtcctag gtgcgtttcg
cgagctgggc gccaggccaa 4380 gccgctccaa aacgcctctc cgactccctc
cagcggcctc catatcccca tccctctcca 4440 cagcaatgtt gttaagcctt
gcaaacgaaa aaatagaaag gctaataagc ttccaatatt 4500 gtggtgtacg
ctgcataacg caacaatgag cgccaaacaa cacacacaca cagcacacag 4560
cagcattaac cacgatgttt aaacagtgta cgcagatccc gtcaacagtt ttatatatcg
4620 tagttacaac catcaacact ttttggtaag tgtaccattc tatactccaa
ctggtctgca 4680 actgtacaag tagacatgtt aatggtagtt aataacatct
acagcagaac ctatggtaaa 4740 gacattgcat ttttacagga agtatcgtcc
tacacgttga taaatccaaa gatgcggaac 4800 ttcttccact tttatcatca
tcccctactc gtacactcgt actctttgtt cgatcgcgat 4860 tcatttctat
aaataatctt gtatgtacat gcggccgcgc ctacttaagc aacgggcttg 4920
ataacagcgg ggggggtgcc cacgttgttg cggttgcgga agaacagaac acccttacca
4980 gcaccctcgg caccagcgct gggctcaacc cactggcaca tacgcgcact
gcggtacatg 5040 gcgcggatga agccacgagg accatcctgg acatcagccc
ggtagtgctt gcccatgatg 5100 ggcttaatgg cctcggtggc ctcgtccgcg
ttgtagaagg ggatgctgct gacgtagtgg 5160 tggaggacat gagtctcgat
gatgccgtgg agaaggtggc ggccgatgaa gcccatctca 5220 cggtcaatgg
tagcagcggc accacggacg aagttccact cgtcgttggt gtagtgggga 5280
agggtagggt cggtgtgctg gaggaaggtg atggcaacga gccagtggtt aacccagagg
5340 tagggaacaa agtaccagat ggccatgttg tagaaaccga acttctgaac
gaggaagtac 5400 agagcagtgg ccatcagacc gataccaata tcgctgagga
cgatgagctt agcgtcactg 5460 ttctcgtaca gagggctgcg gggatcgaag
tggttaacac caccgccgag gccgttatgc 5520 ttgcccttgc cgcgaccctc
acgctggcgc tcgtggtagt tgtggccggt aacattggtg 5580 atgaggtagt
tgggccagcc nacgannnnc tcagtaagat gagcgagctc gtgggtcatc 5640
tttccgagac gagtagcctg ctgctcgcgg gttcggggaa cgaagaccat gtcacgctcc
5700 atgttgccag tggccttgtg gtgctttcgg tgggagattt gccagctgaa
gtaggggaca 5760 aggagggaag agtgaagaac ccagccagta atgtcgttga
tgatgcgaga atcggagaaa 5820 gcaccgtgac cgcactcatg ggcaataacc
cagagaccag taccgaaaag accctgaaga 5880 acggtgtaca cggcccacag
accagcgcgg gcgggggtgg aggggatata ttcgggggtc 5940 acaaagttgt
accagatgct gaaagtggta gtcaggagga caatgtcgcg gaggatataa 6000
ccgtatccct tgagagcgga gcgcttgaag cagtgcttag ggatggcatt gtagatgtcc
6060 ttgatggtaa agtcgggaac ctcgaactgg ttgccgtagg tgtcgagcat
gacaccatac 6120 tcggacttgg gcttggcgat atcaacctcg gacatggacg
agagcgatgt ggaagaggcc 6180 gagtggcggg gagagtctga aggagagacg
gcggcagact cagaatccgt cacagtagtt 6240 gaggtgacgg tgcgtctaag
cgcagggttc tgcttgggca gagccgaagt ggacgccatg 6300 gttgtgaatt
agggtggtga gaatggttgg ttgtagggaa gaatcaaagg ccggtctcgg 6360
gatccgtggg tatatatata tatatatata tatacgatcc ttcgttacct ccctgttctc
6420 aaaactgtgg tttttcgttt ttcgtttttt gctttttttg atttttttag
ggccaactaa 6480 gcttccagat ttcgctaatc acctttgtac taattacaag
aaaggaagaa gctgattaga 6540 gttgggcttt ttatgcaact gtgctactcc
ttatctctga tatgaaagtg tagacccaat 6600 cacatcatgt catttagagt
tggtaatact gggaggatag ataaggcacg aaaacgagcc 6660 atagcagaca
tgctgggtgt agccaagcag aagaaagtag atgggagcca attgacgagc 6720
gagggagcta cgccaatccg acatacgaca cgctgagatc gtcttggccg gggggtacct
6780 acagatgtcc aagggtaagt gcttgactgt aattgtatgt ctgaggacaa
atatgtagtc 6840 agccgtataa agtcatacca ggcaccagtg ccatcatcga
accactaact ctctatgata 6900 catgcctccg gtattattgt accatgcgtc
gctttgttac atacgtatct tgcctttttc 6960 tctcagaaac tccagacttt
ggctattggt cgagataagc ccggaccata gtgagtcttt 7020 cacactctac
atttctccct tgctccaact atttaaattg ccccggagaa gacggccagg 7080
ccgcctagat gacaaattca acaactcaca gctgactttc tgccattgcc actagggggg
7140 ggccttttta tatggccaag ccaagctctc cacgtcggtt gggctgcacc
caacaataaa 7200 tgggtagggt tgcaccaaca aagggatggg atggggggta
gaagatacga ggataacggg 7260 gctcaatggc acaaataaga acgaatactg
ccattaagac tcgtgatcca gcgactgaca 7320 ccattgcatc atctaagggc
ctcaaaacta cctcggaact gctgcgctga tctggacacc 7380 acagaggttc
cgagcacttt aggttgcacc aaatgtccca ccaggtgcag gcagaaaacg 7440
ctggaacagc gtgtacagtt tgtcttaaca aaaagtgagg gcgctgaggt cgagcagggt
7500 ggtgtgactt gttatagcct ttagagctgc gaaagcgcgt atggatttgg
ctcatcaggc 7560 cagattgagg gtctgtggac acatgtcatg ttagtgtact
tcaatcgccc cctggatata 7620 gccccgacaa taggccgtgg cctcattttt
ttgccttccg cacatttcca ttgctcggta 7680 cccacacctt gcttctcctg
cacttgccaa ccttaatact ggtttacatt gaccaacatc 7740
ttacaagcgg ggggcttgtc tagggtatat ataaacagtg gctctcccaa tcggttgcca
7800 gtctcttttt tcctttcttt ccccacagat tcgaaatcta aactacacat
cacagaattc 7860 cgagccgtga gtatccacga caagatcagt gtcgagacga
cgcgttttgt gtaatgacac 7920 aatccgaaag tcgctagcaa cacacactct
ctacacaaac taacccagct ctggtaccat 7980 ggtgaaggct tctcgacagg
ctctgcccct cgtcatcgac ggaaaggtgt acgacgtctc 8040 cgcttgggtg
aacttccacc ctggtggagc tgaaatcatt gagaactacc agggacgaga 8100
tgctactgac gccttcatgg ttatgcactc tcaggaagcc ttcgacaagc tcaagcgaat
8160 gcccaagatc aaccaggctt ccgagctgcc tccccaggct gccgtcaacg
aagctcagga 8220 ggatttccga aagctccgag aagagctgat cgccactggc
atgtttgacg cctctcccct 8280 ctggtactcg tacaagatct tgaccaccct
gggtcttggc gtgcttgcct tcttcatgct 8340 ggtccagtac cacctgtact
tcattggtgc tctcgtgctc ggtatgcact accagcaaat 8400 gggatggctg
tctcatgaca tctgccacca ccagaccttc aagaaccgaa actggaataa 8460
cgtcctgggt ctggtctttg gcaacggact ccagggcttc tccgtgacct ggtggaagga
8520 cagacacaac gcccatcatt ctgctaccaa cgttcagggt cacgatcccg
acattgataa 8580 cctgcctctg ctcgcctggt ccgaggacga tgtcactcga
gcttctccca tctcccgaaa 8640 gctcattcag ttccaacagt actatttcct
ggtcatctgt attctcctgc gattcatctg 8700 gtgtttccag tctgtgctga
ccgttcgatc cctcaaggac cgagacaacc agttctaccg 8760 atctcagtac
aagaaagagg ccattggact cgctctgcac tggactctca agaccctgtt 8820
ccacctcttc tttatgccct ccatcctgac ctcgatgctg gtgttctttg tttccgagct
8880 cgtcggtggc ttcggaattg ccatcgtggt cttcatgaac cactaccctc
tggagaagat 8940 cggtgattcc gtctgggacg gacatggctt ctctgtgggt
cagatccatg agaccatgaa 9000 cattcgacga ggcatcatta ctgactggtt
ctttggaggc ctgaactacc agatcgagca 9060 ccatctctgg cccaccctgc
ctcgacacaa cctcactgcc gtttcctacc aggtggaaca 9120 gctgtgccag
aagcacaacc tcccctaccg aaaccctctg ccccatgaag gtctcgtcat 9180
cctgctccga tacctgtccc agttcgctcg aatggccgag aagcagcccg gtgccaaggc
9240 tcagtaagcg gccgcaagtg tggatgggga agtgagtgcc cggttctgtg
tgcacaattg 9300 gcaatccaag atggatggat tcaacacagg gatatagcga
gctacgtggt ggtgcgagga 9360 tatagcaacg gatatttatg tttgacactt
gagaatgtac gatacaagca ctgtccaagt 9420 acaatactaa acatactgta
catactcata ctcgtacccg ggcaacggtt tcacttgagt 9480 gcagtggcta
gtgctcttac tcgtacagtg tgcaatactg cgtatcatag tctttgatgt 9540
atatcgtatt cattcatgtt agttgcgtac gggtgaagct tccactggtc ggcgtggtag
9600 tggggcagag tggggtcggt gtgctgcagg taggtgatgg ccacgagcca
gtggttgacc 9660 cacaggtagg ggatcaggta gtagagggtg acggaagcca
ggccccatcg gttgatggag 9720 tatgcgatga cggacatggt gataccaata
ccgacgttag agatccagat gttgaaccag 9780 tccttcttct caaacagcgg
ggcgttgggg ttgaagtggt tgacagccca tttgttgagc 9840 ttggggtact
tctgtccggt aacgtaagac agcagataca gaggccatcc aaacacctgc 9900
tgggtgatga ggccgtagag ggtcatgagg ggagcgtcct cagcaagctc agaccagtca
9960 tgggcgcctc ggttctccat aaactccttt cggtccttgg gcacaaacac
catatcacgg 10020 gtgaggtgac cagtggactt gtggtgcatg gagtgggtca
gcttccaggc gtagtaaggg 10080 accagcatgg aggagtgcag aacccatccg
gtgacgttgt tgacggtgtt agagtcggag 10140 aaagcagagt ggccacactc
gtgggcaaga acccacagac cggtgccaaa cagaccctgg 10200 acaatggagt
acatggccca ggccacagct cggccggaag ccgagggaat aagaggcagg 10260
tacgcgtagg ccatgtaggc aaaaacggcg ataaagaagc aggcgcgcca gctgcattaa
10320 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
cgcttcctcg 10380 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
cggtatcagc tcactcaaag 10440 gcggtaatac ggttatccac agaatcaggg
gataacgcag gaaagaacat gtgagcaaaa 10500 ggccagcaaa aggccaggaa
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 10560 cgcccccctg
acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 10620
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
10680 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
ggcgctttct 10740 catagctcac gctgtaggta tctcagttcg gtgtaggtcg
ttcgctccaa gctgggctgt 10800 gtgcacgaac cccccgttca gcccgaccgc
tgcgccttat ccggtaacta tcgtcttgag 10860 tccaacccgg taagacacga
cttatcgcca ctggcagcag ccactggtaa caggattagc 10920 agagcgaggt
atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 10980
actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
11040 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
ttttgtttgc 11100 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
atcctttgat cttttctacg 11160 gggtctgacg ctcagtggaa cgaaaactca
cgttaaggga ttttggtcat gagattatca 11220 aaaaggatct tcacctagat
ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 11280 atatatgagt
aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 11340
gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
11400 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
cccacgctca 11460 ccggctccag atttatcagc aataaaccag ccagccggaa
gggccgagcg cagaagtggt 11520 cctgcaactt tatccgcctc catccagtct
attaattgtt gccgggaagc tagagtaagt 11580 agttcgccag ttaatagttt
gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 11640 cgctcgtcgt
ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 11700
tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
11760 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
ttctcttact 11820 gtcatgccat ccgtaagatg cttttctgtg actggtgagt
actcaaccaa gtcattctga 11880 gaatagtgta tgcggcgacc gagttgctct
tgcccggcgt caatacggga taataccgcg 11940 ccacatagca gaactttaaa
agtgctcatc attggaaaac gttcttcggg gcgaaaactc 12000 tcaaggatct
taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 12060
tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
12120 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
cttccttttt 12180 caatattatt gaagcattta tcagggttat tgtctcatga
gcggatacat atttgaatgt 12240 atttagaaaa ataaacaaat aggggttccg
cgcacatttc cccgaaaagt gccacctgat 12300 gcggtgtgaa ataccgcaca
gatgcgtaag gagaaaatac cgcatcagga aattgtaagc 12360 gttaatattt
tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 12420
taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
12480 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
cgtcaaaggg 12540 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
catcacccta atcaagtttt 12600 ttggggtcga ggtgccgtaa agcactaaat
cggaacccta aagggagccc ccgatttaga 12660 gcttgacggg gaaagccggc
gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 12720 ggcgctaggg
cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 12780
cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
12840 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
tgtgctgcaa 12900 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
acgttgtaaa acgacggcca 12960 gtgaattgta atacgactca ctatagggcg
aattgggccc gacgtcgcat gcttgaatct 13020 acaagtagga gggttggagt
gattaagtga aacttcttta acggctctat gccagttcta 13080 ttgatatccg
aaacatcagt atgaaggtct gataagggtg acttcttccc acagattcgt 13140
atcagtacga gtacgagacc ggtacttgta acagtattga tactaaaggg aaactacaac
13200 ggttgtcagc gtaatgtgac ttcgcccatg aacgcagaca cgcagtgccg
agtgcggtga 13260 tatcgcctac tcgttacgtc catggactac acaacccctc
ggcttcgctt ggcttagcct 13320 cgggctcggt gctgttcagt taaaacacaa
tcaaataaca tttctacttt ttagaaggca 13380 ggccgtcagg agcaactccg
actccattga cgtttctaaa catctgaatg ccttccttac 13440 cttcaacaaa
ctggcaggtt cgggcgacag tgtaaagaga cttgatgaag ttggtgtcgt 13500
cgtgtcggta gtgcttgccc atgaccttct tgatcttctc agtggcgatt cgggcgttgt
13560 agaagggaat tcctttacct gcaggataac ttcgtataat gtatgctata
cgaagttatg 13620 atctctctct tgagcttttc cataacaagt tcttctgcct
ccaggaagtc catgggtggt 13680 ttgatcatgg ttttggtgta gtggtagtgc
agtggtggta ttgtgactgg ggatgtagtt 13740 gagaataagt catacacaag
tcagctttct tcgagcctca tataagtata agtagttcaa 13800 cgtattagca
ctgtacccag catctccgta tcgagaaaca caacaacatg ccccattgga 13860
cagatcatgc ggatacacag gttgtgcagt atcatacata ctcgatcaga caggtcgtct
13920 gaccatcata caagctgaac aagcgctcca tacttgcacg ctctctatat
acacagttaa 13980 attacatatc catagtctaa cctctaacag ttaatcttct
ggtaagcctc ccagccagcc 14040 ttctggtatc gcttggcctc ctcaatagga
tctcggttct ggccgtacag acctcggccg 14100 acaattatga tatccgttcc
ggtagacatg acatcctcaa cagttcggta ctgctgtccg 14160 agagcgtctc
ccttgtcgtc aagacccacc ccgggggtca gaataagcca gtcctcagag 14220
tcgcccttag gtcggttctg ggcaatgaag ccaaccacaa actcggggtc ggatcgggca
14280 agctcaatgg tctgcttgga gtactcgcca gtggccagag agcccttgca
agacagctcg 14340 gccagcatga gcagacctct ggccagcttc tcgttgggag
aggggactag gaactccttg 14400 tactgggagt tctcgtagtc agagacgtcc
tccttcttct gttcagagac agtttcctcg 14460 gcaccagctc gcaggccagc
aatgattccg gttccgggta caccgtgggc gttggtgata 14520 tcggaccact
cggcgattcg gtgacaccgg tactggtgct tgacagtgtt gccaatatct 14580
gcgaactttc tgtcctcgaa caggaagaaa ccgtgcttaa gagcaagttc cttgaggggg
14640 agcacagtgc cggcgtaggt gaagtcgtca atgatgtcga tatgggtttt
gatcatgcac 14700 acataaggtc cgaccttatc ggcaagctca atgagctcct
tggtggtggt aacatccaga 14760 gaagcacaca ggttggtttt cttggctgcc
acgagcttga gcactcgagc ggcaaaggcg 14820 gacttgtgga cgttagctcg
agcttcgtag gagggcattt tggtggtgaa gaggagactg 14880 aaataaattt
agtctgcaga actttttatc ggaaccttat ctggggcagt gaagtatatg 14940
ttatggtaat agttacgagt tagttgaact tatagataga ctggactata cggctatcgg
15000 tccaaattag aaagaacgtc aatggctctc tgggcgtcgc ctttgccgac
aaaaatgtga 15060 tcatgatgaa agccagcaat gacgttgcag ctgatattgt
tgtcggccaa ccgcgccgaa 15120 aacgcagctg tcagacccac agcctccaac
gaagaatgta tcgtcaaagt gatccaagca 15180 cactcatagt tggagtcgta
ctccaaaggc ggcaatgacg agtcagacag atactcgtcg 15240 acgcgataac
ttcgtataat gtatgctata cgaagttatc gtacgatagt tagtagacaa 15300
caat 15304 <210> SEQ ID NO 71 <211> LENGTH: 1272
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: mutant EgD8S-23
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(1270) <223> OTHER INFORMATION: mutant
EgD8S-23 delta-8 desaturase CDS <400> SEQUENCE: 71 catggtgaag
gcttctcgac aggctctgcc cctcgtcatc gacggaaagg tgtacgacgt 60
ctccgcttgg gtgaacttcc accctggtgg agctgaaatc attgagaact accagggacg
120 agatgctact gacgccttca tggttatgca ctctcaggaa gccttcgaca
agctcaagcg 180 aatgcccaag atcaaccagg cttccgagct gcctccccag
gctgccgtca acgaagctca 240 ggaggatttc cgaaagctcc gagaagagct
gatcgccact ggcatgtttg acgcctctcc 300 cctctggtac tcgtacaaga
tcttgaccac cctgggtctt ggcgtgcttg ccttcttcat 360 gctggtccag
taccacctgt acttcattgg tgctctcgtg ctcggtatgc actaccagca 420
aatgggatgg ctgtctcatg acatctgcca ccaccagacc ttcaagaacc gaaactggaa
480 taacgtcctg ggtctggtct ttggcaacgg actccagggc ttctccgtga
cctggtggaa 540 ggacagacac aacgcccatc attctgctac caacgttcag
ggtcacgatc ccgacattga 600 taacctgcct ctgctcgcct ggtccgagga
cgatgtcact cgagcttctc ccatctcccg 660 aaagctcatt cagttccaac
agtactattt cctggtcatc tgtattctcc tgcgattcat 720 ctggtgtttc
cagtctgtgc tgaccgttcg atccctcaag gaccgagaca accagttcta 780
ccgatctcag tacaagaaag aggccattgg actcgctctg cactggactc tcaagaccct
840 gttccacctc ttctttatgc cctccatcct gacctcgatg ctggtgttct
ttgtttccga 900 gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg
aaccactacc ctctggagaa 960 gatcggtgat tccgtctggg acggacatgg
cttctctgtg ggtcagatcc atgagaccat 1020 gaacattcga cgaggcatca
ttactgactg gttctttgga ggcctgaact accagatcga 1080 gcaccatctc
tggcccaccc tgcctcgaca caacctcact gccgtttcct accaggtgga 1140
acagctgtgc cagaagcaca acctccccta ccgaaaccct ctgccccatg aaggtctcgt
1200 catcctgctc cgatacctgt cccagttcgc tcgaatggcc gagaagcagc
ccggtgccaa 1260 ggctcagtaa gc 1272 <210> SEQ ID NO 72
<211> LENGTH: 422 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: mutant EgD8S-23, comprising M1, M2, M3, M8, M12, M15,
M16, M18, M21, M26, M45, M46, M68 and M70 mutation sites
<400> SEQUENCE: 72 Met Val Lys Ala Ser Arg Gln Ala Leu Pro
Leu Val Ile Asp Gly Lys 1 5 10 15 Val Tyr Asp Val Ser Ala Trp Val
Asn Phe His Pro Gly Gly Ala Glu 20 25 30 Ile Ile Glu Asn Tyr Gln
Gly Arg Asp Ala Thr Asp Ala Phe Met Val 35 40 45 Met His Ser Gln
Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 50 55 60 Asn Gln
Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 65 70 75 80
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 85
90 95 Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu
Gly 100 105 110 Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His
Leu Tyr Phe 115 120 125 Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln
Gln Met Gly Trp Leu 130 135 140 Ser His Asp Ile Cys His His Gln Thr
Phe Lys Asn Arg Asn Trp Asn 145 150 155 160 Asn Val Leu Gly Leu Val
Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 165 170 175 Thr Trp Trp Lys
Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 180 185 190 Gln Gly
His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 195 200 205
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 210
215 220 Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe
Ile 225 230 235 240 Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu
Lys Asp Arg Asp 245 250 255 Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys
Glu Ala Ile Gly Leu Ala 260 265 270 Leu His Trp Thr Leu Lys Thr Leu
Phe His Leu Phe Phe Met Pro Ser 275 280 285 Ile Leu Thr Ser Met Leu
Val Phe Phe Val Ser Glu Leu Val Gly Gly 290 295 300 Phe Gly Ile Ala
Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 305 310 315 320 Ile
Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 325 330
335 His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe
340 345 350 Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr
Leu Pro 355 360 365 Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu
Gln Leu Cys Gln 370 375 380 Lys His Asn Leu Pro Tyr Arg Asn Pro Leu
Pro His Glu Gly Leu Val 385 390 395 400 Ile Leu Leu Arg Tyr Leu Ser
Gln Phe Ala Arg Met Ala Glu Lys Gln 405 410 415 Pro Gly Ala Lys Ala
Gln 420 <210> SEQ ID NO 73 <211> LENGTH: 777
<212> TYPE: DNA <213> ORGANISM: Euglena gracilis
<220> FEATURE: <221> NAME/KEY: misc_feature <223>
OTHER INFORMATION: delta-9 elongase <300> PUBLICATION
INFORMATION: <302> TITLE: DELTA-9 ELONGASES AND THEIR USE IN
MAKING POLYUNSATURATED FATTY ACIDS <310> PATENT DOCUMENT
NUMBER: WO 2007/061742 <311> PATENT FILING DATE: 2006-11-16
<312> PUBLICATION DATE: 2007-05-31 <313> RELEVANT
RESIDUES IN SEQ ID NO: (1)..(777) <400> SEQUENCE: 73
atggaggtgg tgaatgaaat agtctcaatt gggcaggaag ttttacccaa agttgattat
60 gcccaactct ggagtgatgc cagtcactgt gaggtgcttt acttgtccat
cgcatttgtc 120 atcttgaagt tcactcttgg cccccttggt ccaaaaggtc
agtctcgtat gaagtttgtt 180 ttcaccaatt acaaccttct catgtccatt
tattcgttgg gatcattcct ctcaatggca 240 tatgccatgt acaccatcgg
tgttatgtct gacaactgcg agaaggcttt tgacaacaac 300 gtcttcagga
tcaccacgca gttgttctat ttgagcaagt tcctggagta tattgactcc 360
ttctatttgc cactgatggg caagcctctg acctggttgc aattcttcca tcatttgggg
420 gcaccgatgg atatgtggct gttctataat taccgaaatg aagctgtttg
gatttttgtg 480 ctgttgaatg gtttcatcca ctggatcatg tacggttatt
attggaccag attgatcaag 540 ctgaagttcc ccatgccaaa atccctgatt
acatcaatgc agatcattca attcaatgtt 600 ggtttctaca ttgtctggaa
gtacaggaac attccctgtt atcgccaaga tgggatgagg 660 atgtttggct
ggttcttcaa ttacttttat gttggcacag tcttgtgttt gttcttgaat 720
ttctatgtgc aaacgtatat cgtcaggaag cacaagggag ccaaaaagat tcagtga 777
<210> SEQ ID NO 74 <211> LENGTH: 13707 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Plasmid pZKSL-555R
<400> SEQUENCE: 74 aaacagtgta cgcagatctg cccatgatgg
gggctcccac caccagcaat cagggccctg 60 attacacacc cacctgtaat
gtcatgctgt tcatcgtggt taatgctgct gtgtgctgtg 120 tgtgtgtgtt
gtttggcgct cattgttgcg ttatgcagcg tacaccacaa tattggaagc 180
ttattagcct ttctattttt tcgtttgcaa ggcttaacaa cattgctgtg gagagggatg
240 gggatatgga ggccgctgga gggagtcgga gaggcgtttt ggagcggctt
ggcctggcgc 300 ccagctcgcg aaacgcacct aggacccttt ggcacgccga
aatgtgccac ttttcagtct 360 agtaacgcct tacctacgtc attccatgcg
tgcatgtttg cgcctttttt cccttgccct 420 tgatcgccac acagtacagt
gcactgtaca gtggaggttt tgggggggtc ttagatggga 480 gctaaaagcg
gcctagcggt acactagtgg gattgtatgg agtggcatgg agcctaggtg 540
gagcctgaca ggacgcacga ccggctagcc cgtgacagac gatgggtggc tcctgttgtc
600 caccgcgtac aaatgtttgg gccaaagtct tgtcagcctt gcttgcgaac
ctaattccca 660 attttgtcac ttcgcacccc cattgatcga gccctaaccc
ctgcccatca ggcaatccaa 720 ttaagctcgc attgtctgcc ttgtttagtt
tggctcctgc ccgtttcggc gtccacttgc 780 acaaacacaa acaagcatta
tatataaggc tcgtctctcc ctcccaacca cactcacttt 840 tttgcccgtc
ttcccttgct aacacaaaag tcaagaacac aaacaaccac cccaaccccc 900
ttacacacaa gacatatcta cagcaatggc catggctctc tcccttacta ccgagcagct
960 gctcgagcga cccgacctgg ttgccatcga cggcattctc tacgatctgg
aaggtcttgc 1020 caaggtccat cccggaggcg acttgatcct cgcttctggt
gcctccgatg cttctcctct 1080 gttctactcc atgcaccctt acgtcaagcc
cgagaactcg aagctgcttc aacagttcgt 1140 gcgaggcaag cacgaccgaa
cctccaagga cattgtctac acctacgact ctccctttgc 1200 acaggacgtc
aagcgaacta tgcgagaggt catgaaaggt cggaactggt atgccacacc 1260
tggattctgg ctgcgaaccg ttggcatcat tgctgtcacc gccttttgcg agtggcactg
1320 ggctactacc ggaatggtgc tgtggggtct cttgactgga ttcatgcaca
tgcagatcgg 1380 cctgtccatt cagcacgatg cctctcatgg tgccatcagc
aaaaagccct gggtcaacgc 1440 tctctttgcc tacggcatcg acgtcattgg
atcgtccaga tggatctggc tgcagtctca 1500 catcatgcga catcacacct
acaccaatca gcatggtctc gacctggatg ccgagtccgc 1560 agaaccattc
cttgtgttcc acaactaccc tgctgccaac actgctcgaa agtggtttca 1620
ccgattccag gcctggtaca tgtacctcgt gcttggagcc tacggcgttt cgctggtgta
1680 caaccctctc tacatcttcc gaatgcagca caacgacacc attcccgagt
ctgtcacagc 1740 catgcgagag aacggctttc tgcgacggta ccgaaccctt
gcattcgtta tgcgagcttt 1800 cttcatcttt cgaaccgcct tcttgccctg
gtatctcact ggaacctccc tgctcatcac 1860 cattcctctg gtgcccactg
ctaccggtgc cttcctcacc ttctttttca tcttgtctca 1920 caacttcgat
ggctcggagc gaatccccga caagaactgc aaggtcaaga gctccgagaa 1980
ggacgttgaa gccgatcaga tcgactggta cagagctcag gtggagacct cttccaccta
2040 cggtggaccc attgccatgt tctttactgg cggtctcaac ttccagatcg
agcatcacct 2100 ctttcctcga atgtcgtctt ggcactatcc cttcgtgcag
caagctgtcc gagagtgttg 2160 cgaacgacac ggagttcggt acgtcttcta
ccctaccatt gtgggcaaca tcatttccac 2220 cctcaagtac atgcacaaag
tcggtgtggt tcactgtgtc aaggacgctc aggattccta 2280 agcggccgca
agtgtggatg gggaagtgag tgcccggttc tgtgtgcaca attggcaatc 2340
caagatggat ggattcaaca cagggatata gcgagctacg tggtggtgcg aggatatagc
2400 aacggatatt tatgtttgac acttgagaat gtacgataca agcactgtcc
aagtacaata 2460 ctaaacatac tgtacatact catactcgta cccgggcaac
ggtttcactt gagtgcagtg 2520 gctagtgctc ttactcgtac agtgtgcaat
actgcgtatc atagtctttg atgtatatcg 2580 tattcattca tgttagttgc
gtacgctgtg ttgttgtatg tggtgaagct tgacaatgga 2640 tggtgtgtcg
tatcaggctg gggaacaatt gtgcttaagt atgctgcagt tgagtaagag 2700
tcatcgctcc accaaaataa agtttgccat tagggttgga gagagagatg gtggctggaa
2760 gaattaaatg acatcaagct gaggattgtg ggtgtgcaat aacacatgtt
aggggtgacc 2820 tgtggctcga aatctgataa ttattttgta actttatgat
tattcttaga ttttttaata 2880 ttcctctata taacacataa gtagctgtcg
tctagttgtt catagcctga ctcctgcaat 2940 agattagtgc agagtgattt
tgtgcaattg agagccacgg ttgagtcaag tgactttgtg 3000 tgtgaagtca
tcttacgttt caagtctcac aggttactca attggttggt tgtctgccct 3060
ttacagatat ttacagtacc tgagcgtaaa gtcgttcatc cacggaatga ctgttcctgt
3120 cacgcagtca tgatcatgga tgtggctggt caggaaccat tttggatagg
agacttaggg 3180 attggactat tattgaaaaa actgagccga atatgatata
gttctatttg aatgcagaac 3240 ttctgatggt caattcactt atttcaggca
tatcggtcat ggtggcagct gccacgatgt 3300 tatctcgttg gaaacctcgg
cgcgccagct gcattaatga atcggccaac gcgcggggag 3360 aggcggtttg
cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 3420
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
3480 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
ccaggaaccg 3540 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
ccccctgacg agcatcacaa 3600 aaatcgacgc tcaagtcaga ggtggcgaaa
cccgacagga ctataaagat accaggcgtt 3660 tccccctgga agctccctcg
tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3720 gtccgccttt
ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3780
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
3840 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
gacacgactt 3900 atcgccactg gcagcagcca ctggtaacag gattagcaga
gcgaggtatg taggcggtgc 3960 tacagagttc ttgaagtggt ggcctaacta
cggctacact agaagaacag tatttggtat 4020 ctgcgctctg ctgaagccag
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4080 acaaaccacc
gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4140
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
4200 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
cctagatcct 4260 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
tatgagtaaa cttggtctga 4320 cagttaccaa tgcttaatca gtgaggcacc
tatctcagcg atctgtctat ttcgttcatc 4380 catagttgcc tgactccccg
tcgtgtagat aactacgata cgggagggct taccatctgg 4440 ccccagtgct
gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4500
aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
4560 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
atagtttgcg 4620 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
tcgtcgtttg gtatggcttc 4680 attcagctcc ggttcccaac gatcaaggcg
agttacatga tcccccatgt tgtgcaaaaa 4740 agcggttagc tccttcggtc
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4800 actcatggtt
atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4860
ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
4920 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
ctttaaaagt 4980 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
aggatcttac cgctgttgag 5040 atccagttcg atgtaaccca ctcgtgcacc
caactgatct tcagcatctt ttactttcac 5100 cagcgtttct gggtgagcaa
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 5160 gacacggaaa
tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 5220
gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
5280 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata
ccgcacagat 5340 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
aatattttgt taaaattcgc 5400 gttaaatttt tgttaaatca gctcattttt
taaccaatag gccgaaatcg gcaaaatccc 5460 ttataaatca aaagaataga
ccgagatagg gttgagtgtt gttccagttt ggaacaagag 5520 tccactatta
aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 5580
tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
5640 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
agccggcgaa 5700 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
gctagggcgc tggcaagtgt 5760 agcggtcacg ctgcgcgtaa ccaccacacc
cgccgcgctt aatgcgccgc tacagggcgc 5820 gtccattcgc cattcaggct
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 5880 ctattacgcc
agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 5940
gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
6000 tagggcgaat tgggcccgac gtcgcatgca ttccatagcc acacctttgc
ctatggcttc 6060 acaaccgaag gcaattcgag aggtcgcgct tatggaatcg
actcgtataa agctgaaggg 6120 aaagggagac gttccgagcg ctcagatgca
atagtcgtcc agctaatgtg gattcaaaaa 6180 caaccccaac agtaatcttg
aaaatttgaa cggatcaatc tgaacactct tgctccaggt 6240 cattcttcta
acgcacatcc ccagagtcta gagggagttg tgttgtgaac atcctaataa 6300
acaatgcaat ggattcggga tatcttctgt ctcgccccct actcgatgtc gagtaaaccg
6360 atcaccaact aacaatactc ctccgcgttc tgccattgac tctcaaacag
acatcgctat 6420 caacggaaca gcatatttta gcttcttagg acaataaata
ttgataatgc cggctctccc 6480 tcggtatatt aagcaatcca ttcatacact
cattcatcag gttaatttta tatatataat 6540 ttgtctattc aaacaccgta
aattactggt accatcatct cctccttttc aaatacacgt 6600 ctatttgcat
taatgaaatt actcgccaat tcgcagaacg tgtttgtcga acagagcctt 6660
agctcgggtc cagacaggag cagtgtctcg ctgaggaagc tgcaggagag ttaattaact
6720 cacctgcagg attgagacta tgaatggatt cccgtgcccg tattactcta
ctaatttgat 6780 cttggaacgc gaaaatacgt ttctaggact ccaaagaatc
tcaactcttg tccttactaa 6840 atatactacc catagttgat ggtttacttg
aacagagagg acatgttcac ttgacccaaa 6900 gtttctcgca tctcttggat
atttgaacaa cggcgtccac tgaccgtcag ttatccagtc 6960 acaaaacccc
cacattcata cattcccatg tacgtttaca aagttctcaa ttccatcgtg 7020
caaatcaaaa tcacatctat tcattcatca tatataaacc catcatgtct actaacactc
7080 acaactccat agaaaacatc gactcagaac acacgctcca tgcggccgct
taggaatcct 7140 gtgcgtcctt cacgcagtgg acgacaccca ccttatgcat
gtacttcagg gtggagatga 7200 tgttgccgac gatggtaggg tagaaaacat
atcgcactcc atgtcgttcg caacactccc 7260 ggaccgcctg ctggacgaag
gggtagtgcc aagacgacat ccggggaaag aggtggtgct 7320 cgatctggaa
attgagaccg ccagtgaaga acatggcgat ggggccaccg tatgtggagg 7380
acgtctccac ctgcgcccga taccagtcaa tttggtcagc ctcaacgtcc ttctcagatc
7440 gcttaacctt gcagttcttg tcggggatcc gttcggagcc atcaaaattg
tgggacaaaa 7500 tgaagaagaa cgtcaagaag gcaccagttg cggtgggcac
cagaggaatg gtgatcagca 7560 atgaggtccc agtgaggtac cagggcaaga
atgcggtccg gaagatgaag aaagctcgca 7620 tcacgaatgc aagtgtgcgg
tagcgccgca gaaagccatt ttcccgcatg gccgtgacag 7680 actctgggat
ggtgtcattg tgctgcatcc ggaaaatgta gagcgggttg tacaccagcg 7740
ataccccgta tgcccccagc acaaggtaca tgtaccaagc ctggaagcgg tggaaccact
7800 ttcgggcggt gtttgcggcg gggtagttgt ggaacaccag gaacggctct
gccgactccg 7860 catccaggtc gaggccgtgc tggttggtgt aggtgtggtg
ccgcatgatg tgcgactgca 7920 gccaaatcca ccgggacgat ccgatgacgt
caatgccgta ggcgaagagg gcgttgaccc 7980 aaggcttctt gctgatggcc
ccgtgggacg catcatgctg gatggataag ccgatctgca 8040 tgtgcatgaa
tccagtcaac aggccccaca gcaccatccc cgtggtagcc cagtgccact 8100
cgcaaaaggc cgtcacggcg atgatcccaa cggtgcgcag ccagaagcca ggggttgcgt
8160 accagttcct ccctttcatc acctcgcgca ttgtccgctt aacgtcttgt
gcgaagggag 8220 aatcatacgt gtagacaatg tccttcgagg tgcggtcatg
cttccctcgg acgaactgtt 8280 gaagcaattt ggagttctcc ggtttgacgt
atggatgcat tgaataaaag agaggggagg 8340 catcagaggc accagaagcg
agaatcaaat ctcctcctgg atgaactttg gcaagccctt 8400 caaggtcgta
gaggatgcca tcaatcgcaa ccaaatcagg gcgttctaac agctgttctg 8460
tggtaagact gagagccatg gagagctggg ttagtttgtg tagagagtgt gtgttgctag
8520 cgactttcgg attgtgtcat tacacaaaac gcgtcgtctc gacactgatc
ttgtcgtgga 8580 tactcacggc tcggacatcg tcgccgacga tgacaccgga
ctttcgctta aggacgtcag 8640 taacaggcat tgtgtgatgt gtagtttaga
tttcgaatct gtggggaaag aaaggaaaaa 8700 agagactggc aaccgattgg
gagagccact gtttatatat accctagaca agccccccgc 8760 ttgtaagatg
ttggtcaatg taaaccagta ttaaggttgg caagtgcagg agaagcaagg 8820
tgtgggtacc gagcaatgga aatgtgcgga aggcaaaaaa atgaggccac ggcctattgt
8880 cggggctata tccagggggc gattgaagta cactaacatg acatgtgtcc
acagaccctc 8940 aatctggcct gatgagccaa atccatacgc gctttcgcag
ctctaaaggc tataacaagt 9000 cacaccaccc tgctcgacct cagcgccctc
actttttgtt aagacaaact gtacacgctg 9060 ttccagcgtt ttctgcctgc
acctggtggg acatttggtg caacctaaag tgctcggaac 9120 ctctgtggtg
tccagatcag cgcagcagtt ccgaggtagt tttgaggccc ttagatgatg 9180
caatggtgtc agtcgctgga tcacgagtct taatggcagt attcgttctt atttgtgcca
9240 ttgagccccg ttatcctcgt atcttctacc ccccatccca tccctttgtt
ggtgcaaccc 9300 tacccattta ttgttgggtg cagcccaacc gacgtggaga
gcttggcttg gccatataaa 9360 aaggcccccc cctagtggca atggcagaaa
gtcagctgtg agttgttgaa tttgtcatct 9420 aggcggcctg gccgtcttct
ccggggcaat tggggctgtt ttttgggaca caaatacgcc 9480 gccaacccgg
tctctcctga attccgtcgt cgcctgagtc gacatcattt atttaccagt 9540
tggccacaaa cccttgacga tctcgtatgt cccctccgac atactcccgg ccggctgggg
9600 tacgttcgat agcgctatcg gcatcgacaa ggtttgggtc cctagccgat
accgcactac 9660 ctgagtcaca atcttcggag gtttagtctt ccacatagca
cgggcaaaag tgcgtatata 9720 tacaagagcg tttgccagcc acagattttc
actccacaca ccacatcaca catacaacca 9780 cacacatcca caatggaacc
cgaaactaag aagaccaaga ctgactccaa gaagattgtt 9840 cttctcggcg
gcgacttctg tggccccgag gtgattgccg aggccgtcaa ggtgctcaag 9900
tctgttgctg aggcctccgg caccgagttt gtgtttgagg accgactcat tggaggagct
9960 gccattgaga aggagggcga gcccatcacc gacgctactc tcgacatctg
ccgaaaggct 10020 gactctatta tgctcggtgc tgtcggaggc gctgccaaca
ccgtatggac cactcccgac 10080 ggacgaaccg acgtgcgacc cgagcagggt
ctcctcaagc tgcgaaagga cctgaacctg 10140 tacgccaacc tgcgaccctg
ccagctgctg tcgcccaagc tcgccgatct ctcccccatc 10200 cgaaacgttg
agggcaccga cttcatcatt gtccgagagc tcgtcggagg tatctacttt 10260
ggagagcgaa aggaggatga cggatctggc gtcgcttccg acaccgagac ctactccgtt
10320 cctgaggttg agcgaattgc ccgaatggcc gccttcctgg cccttcagca
caacccccct 10380 cttcccgtgt ggtctcttga caaggccaac gtgctggcct
cctctcgact ttggcgaaag 10440 actgtcactc gagtcctcaa ggacgaactc
ccccagctcg agctcaacca ccagctgatc 10500 gactcggccg ccatgatcct
catcaagcag ccctccaaga tgaatggtat catcatcacc 10560 accaacatgt
ttggcgatat catctccgac gaggcctccg tcatccccgg ttctctgggt 10620
ctgctgccct ccgcctctct ggcttctctg cccgacacca acgaggcgtt cggtctgtac
10680 gagccctgtc acggatctgc ccccgatctc ggcaagcaga aggtcaaccc
cattgccacc 10740 attctgtctg ccgccatgat gctcaagttc tctcttaaca
tgaagcccgc cggtgacgct 10800 gttgaggctg ccgtcaagga gtccgtcgag
gctggtatca ctaccgccga tatcggaggc 10860 tcttcctcca cctccgaggt
cggagacttg ttgccaacaa ggtcaaggag ctgctcaaga 10920 aggagtaagt
cgtttctacg acgcattgat ggaaggagca aactgacgcg cctgcgggtt 10980
ggtctaccgg cagggtccgc tagtgtataa gactctataa aaagggccct gccctgctaa
11040 tgaaatgatg atttataatt taccggtgta gcaaccttga ctagaagaag
cagattgggt 11100 gtgtttgtag tggaggacag tggtacgttt tggaaacagt
cttcttgaaa gtgtcttgtc 11160 tacagtatat tcactcataa cctcaatagc
caagggtgta gtcggtttat taaaggaagg 11220 gagttgtggc tgatgtggat
atcgatagtt ggagcaaggg agaaatgtag agtgtgaaag 11280 actcactatg
gtccgggctt atctcgacca atagccaaag tctggagttt ctgagagaaa 11340
aaggcaagat acgtatgtaa caaagcgacg catggtacaa taataccgga ggcatgtatc
11400 atagagagtt agtggttcga tgatggcact ggtgcctggt atgactttat
acggctgact 11460 acatatttgt cctcagacat acaattacag tcaagcactt
acccttggac atctgtaggt 11520 accccccggc caagacgatc tcagcgtgtc
gtatgtcgga ttggcgtagc tccctcgctc 11580 gtcaattggc tcccatctac
tttcttctgc ttggctacac ccagcatgtc tgctatggct 11640 cgttttcgtg
ccttatctat cctcccagta ttaccaactc taaatgacat gatgtgattg 11700
ggtctacact ttcatatcag agataaggag tagcacagtt gcataaaaag cccaactcta
11760 atcagcttct tcctttcttg taattagtac aaaggtgatt agcgaaatct
ggaagcttag 11820 ttggccctaa aaaaatcaaa aaaagcaaaa aacgaaaaac
gaaaaaccac agttttgaga 11880 acagggaggt aacgaaggat cgtatatata
tatatatata tatataccca cggatcccga 11940 gaccggcctt tgattcttcc
ctacaaccaa ccattctcac caccctaatt cacaaccatg 12000 gctcccgacg
ccgacaagct gcgacagcga aaggctcagt ccatccagga cactgccgat 12060
tctcaggcta ccgagctcaa gattggcacc ctgaagggtc tccaaggcac cgagatcgtc
12120 attgatggcg acatctacga catcaaagac ttcgatcacc ctggaggcga
atccatcatg 12180 acctttggtg gcaacgacgt tactgccacc tacaagatga
ttcatcccta ccactcgaag 12240 catcacctgg agaagatgaa aaaggtcggt
cgagtgcccg actacacctc cgagtacaag 12300 ttcgatactc ccttcgaacg
agagatcaaa caggaggtct tcaagattgt gcgaagaggt 12360 cgagagtttg
gaacacctgg ctacttcttt cgagccttct gctacatcgg tctcttcttt 12420
tacctgcagt atctctgggt taccactcct accactttcg cccttgctat cttctacggt
12480 gtgtctcagg ccttcattgg cctgaacgtc cagcacgacg ccaaccacgg
agctgcctcc 12540 aaaaagccct ggatcaacaa tttgctcggc ctgggtgccg
actttatcgg aggctccaag 12600 tggctctgga tgaaccagca ctggacccat
cacacttaca ccaaccatca cgagaaggat 12660 cccgacgccc tgggtgcaga
gcctatgctg ctcttcaacg actatccctt gggtcacccc 12720 aagcgaaccc
tcattcatca cttccaagcc ttctactatc tgtttgtcct tgctggctac 12780
tgggtgtctt cggtgttcaa ccctcagatc ctggacctcc agcaccgagg tgcccaggct
12840 gtcggcatga agatggagaa cgactacatt gccaagtctc gaaagtacgc
tatcttcctg 12900 cgactcctgt acatctacac caacattgtg gctcccatcc
agaaccaagg cttttcgctc 12960 accgtcgttg ctcacattct tactatgggt
gtcgcctcca gcctgaccct cgctactctg 13020 ttcgccctct cccacaactt
cgagaacgca gatcgggatc ccacctacga ggctcgaaag 13080 ggaggcgagc
ctgtctgttg gttcaagtcg caggtggaaa cctcctctac ttacggtggc 13140
ttcatttccg gttgccttac aggcggactc aactttcagg tcgagcatca cctgtttcct
13200 cgaatgtcct ctgcctggta cccctacatc gctcctaccg ttcgagaggt
ctgcaaaaag 13260 cacggcgtca agtacgccta ctatccctgg gtgtggcaga
acctcatctc gaccgtcaag 13320 tacctgcatc agtccggaac tggctcgaac
tggaagaacg gtgccaatcc ctactctggc 13380 aagctgtaag cggccgcatg
tacatacaag attatttata gaaatgaatc gcgatcgaac 13440 aaagagtacg
agtgtacgag taggggatga tgataaaagt ggaagaagtt ccgcatcttt 13500
ggatttatca acgtgtagga cgatacttcc tgtaaaaatg caatgtcttt accataggtt
13560 ctgctgtaga tgttattaac taccattaac atgtctactt gtacagttgc
agaccagttg 13620 gagtatagaa tggtacactt accaaaaagt gttgatggtt
gtaactacga tatataaaac 13680 tgttgacggg atctgcgtac actgttt 13707
<210> SEQ ID NO 75 <211> LENGTH: 1350 <212> TYPE:
DNA <213> ORGANISM: Euglena gracilis <220> FEATURE:
<221> NAME/KEY: misc_feature <223> OTHER INFORMATION:
synthetic delta-5 desaturase (codon-optimized) for Yarrowia
lipolytica <400> SEQUENCE: 75 atggctctct cccttactac
cgagcagctg ctcgagcgac ccgacctggt tgccatcgac 60 ggcattctct
acgatctgga aggtcttgcc aaggtccatc ccggaggcga cttgatcctc 120
gcttctggtg cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc
180 gagaactcga agctgcttca acagttcgtg cgaggcaagc acgaccgaac
ctccaaggac 240 attgtctaca cctacgactc tccctttgca caggacgtca
agcgaactat gcgagaggtc 300 atgaaaggtc ggaactggta tgccacacct
ggattctggc tgcgaaccgt tggcatcatt 360 gctgtcaccg ccttttgcga
gtggcactgg gctactaccg gaatggtgct gtggggtctc 420 ttgactggat
tcatgcacat gcagatcggc ctgtccattc agcacgatgc ctctcatggt 480
gccatcagca aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga
540 tcgtccagat ggatctggct gcagtctcac atcatgcgac atcacaccta
caccaatcag 600 catggtctcg acctggatgc cgagtccgca gaaccattcc
ttgtgttcca caactaccct 660 gctgccaaca ctgctcgaaa gtggtttcac
cgattccagg cctggtacat gtacctcgtg 720 cttggagcct acggcgtttc
gctggtgtac aaccctctct acatcttccg aatgcagcac 780 aacgacacca
ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac 840
cgaacccttg cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg
900 tatctcactg gaacctccct gctcatcacc attcctctgg tgcccactgc
taccggtgcc 960 ttcctcacct tctttttcat cttgtctcac aacttcgatg
gctcggagcg aatccccgac 1020 aagaactgca aggtcaagag ctccgagaag
gacgttgaag ccgatcagat cgactggtac 1080 agagctcagg tggagacctc
ttccacctac ggtggaccca ttgccatgtt ctttactggc 1140 ggtctcaact
tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc 1200
ttcgtgcagc aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac
1260 cctaccattg tgggcaacat catttccacc ctcaagtaca tgcacaaagt
cggtgtggtt 1320 cactgtgtca aggacgctca ggattcctaa 1350 <210>
SEQ ID NO 76 <211> LENGTH: 449 <212> TYPE: PRT
<213> ORGANISM: Euglena gracilis <400> SEQUENCE: 76 Met
Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 1 5 10
15 Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val
20 25 30 His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp
Ala Ser 35 40 45 Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro
Glu Asn Ser Lys 50 55 60 Leu Leu Gln Gln Phe Val Arg Gly Lys His
Asp Arg Thr Ser Lys Asp 65 70 75 80 Ile Val Tyr Thr Tyr Asp Ser Pro
Phe Ala Gln Asp Val Lys Arg Thr 85 90 95 Met Arg Glu Val Met Lys
Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe
100 105 110 Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys
Glu Trp 115 120 125 His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu
Leu Thr Gly Phe 130 135 140 Met His Met Gln Ile Gly Leu Ser Ile Gln
His Asp Ala Ser His Gly 145 150 155 160 Ala Ile Ser Lys Lys Pro Trp
Val Asn Ala Leu Phe Ala Tyr Gly Ile 165 170 175 Asp Val Ile Gly Ser
Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 180 185 190 Arg His His
Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 195 200 205 Ser
Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 210 215
220 Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val
225 230 235 240 Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu
Tyr Ile Phe 245 250 255 Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser
Val Thr Ala Met Arg 260 265 270 Glu Asn Gly Phe Leu Arg Arg Tyr Arg
Thr Leu Ala Phe Val Met Arg 275 280 285 Ala Phe Phe Ile Phe Arg Thr
Ala Phe Leu Pro Trp Tyr Leu Thr Gly 290 295 300 Thr Ser Leu Leu Ile
Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 305 310 315 320 Phe Leu
Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 325 330 335
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 340
345 350 Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser
Ser 355 360 365 Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly
Leu Asn Phe 370 375 380 Gln Ile Glu His His Leu Phe Pro Arg Met Ser
Ser Trp His Tyr Pro 385 390 395 400 Phe Val Gln Gln Ala Val Arg Glu
Cys Cys Glu Arg His Gly Val Arg 405 410 415 Tyr Val Phe Tyr Pro Thr
Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 420 425 430 Tyr Met His Lys
Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 435 440 445 Ser
<210> SEQ ID NO 77 <211> LENGTH: 1392 <212> TYPE:
DNA <213> ORGANISM: Peridinium sp. CCMP626 <220>
FEATURE: <221> NAME/KEY: misc_feature <223> OTHER
INFORMATION: synthetic delta-5 desaturase (codon-optimized) for
Yarrowia lipolytica <400> SEQUENCE: 77 atggctcccg acgccgacaa
gctgcgacag cgaaaggctc agtccatcca ggacactgcc 60 gattctcagg
ctaccgagct caagattggc accctgaagg gtctccaagg caccgagatc 120
gtcattgatg gcgacatcta cgacatcaaa gacttcgatc accctggagg cgaatccatc
180 atgacctttg gtggcaacga cgttactgcc acctacaaga tgattcatcc
ctaccactcg 240 aagcatcacc tggagaagat gaaaaaggtc ggtcgagtgc
ccgactacac ctccgagtac 300 aagttcgata ctcccttcga acgagagatc
aaacaggagg tcttcaagat tgtgcgaaga 360 ggtcgagagt ttggaacacc
tggctacttc tttcgagcct tctgctacat cggtctcttc 420 ttttacctgc
agtatctctg ggttaccact cctaccactt tcgcccttgc tatcttctac 480
ggtgtgtctc aggccttcat tggcctgaac gtccagcacg acgccaacca cggagctgcc
540 tccaaaaagc cctggatcaa caatttgctc ggcctgggtg ccgactttat
cggaggctcc 600 aagtggctct ggatgaacca gcactggacc catcacactt
acaccaacca tcacgagaag 660 gatcccgacg ccctgggtgc agagcctatg
ctgctcttca acgactatcc cttgggtcac 720 cccaagcgaa ccctcattca
tcacttccaa gccttctact atctgtttgt ccttgctggc 780 tactgggtgt
cttcggtgtt caaccctcag atcctggacc tccagcaccg aggtgcccag 840
gctgtcggca tgaagatgga gaacgactac attgccaagt ctcgaaagta cgctatcttc
900 ctgcgactcc tgtacatcta caccaacatt gtggctccca tccagaacca
aggcttttcg 960 ctcaccgtcg ttgctcacat tcttactatg ggtgtcgcct
ccagcctgac cctcgctact 1020 ctgttcgccc tctcccacaa cttcgagaac
gcagatcggg atcccaccta cgaggctcga 1080 aagggaggcg agcctgtctg
ttggttcaag tcgcaggtgg aaacctcctc tacttacggt 1140 ggcttcattt
ccggttgcct tacaggcgga ctcaactttc aggtcgagca tcacctgttt 1200
cctcgaatgt cctctgcctg gtacccctac atcgctccta ccgttcgaga ggtctgcaaa
1260 aagcacggcg tcaagtacgc ctactatccc tgggtgtggc agaacctcat
ctcgaccgtc 1320 aagtacctgc atcagtccgg aactggctcg aactggaaga
acggtgccaa tccctactct 1380 ggcaagctgt aa 1392 <210> SEQ ID NO
78 <211> LENGTH: 463 <212> TYPE: PRT <213>
ORGANISM: Peridinium sp. CCMP626 <400> SEQUENCE: 78 Met Ala
Pro Asp Ala Asp Lys Leu Arg Gln Arg Lys Ala Gln Ser Ile 1 5 10 15
Gln Asp Thr Ala Asp Ser Gln Ala Thr Glu Leu Lys Ile Gly Thr Leu 20
25 30 Lys Gly Leu Gln Gly Thr Glu Ile Val Ile Asp Gly Asp Ile Tyr
Asp 35 40 45 Ile Lys Asp Phe Asp His Pro Gly Gly Glu Ser Ile Met
Thr Phe Gly 50 55 60 Gly Asn Asp Val Thr Ala Thr Tyr Lys Met Ile
His Pro Tyr His Ser 65 70 75 80 Lys His His Leu Glu Lys Met Lys Lys
Val Gly Arg Val Pro Asp Tyr 85 90 95 Thr Ser Glu Tyr Lys Phe Asp
Thr Pro Phe Glu Arg Glu Ile Lys Gln 100 105 110 Glu Val Phe Lys Ile
Val Arg Arg Gly Arg Glu Phe Gly Thr Pro Gly 115 120 125 Tyr Phe Phe
Arg Ala Phe Cys Tyr Ile Gly Leu Phe Phe Tyr Leu Gln 130 135 140 Tyr
Leu Trp Val Thr Thr Pro Thr Thr Phe Ala Leu Ala Ile Phe Tyr 145 150
155 160 Gly Val Ser Gln Ala Phe Ile Gly Leu Asn Val Gln His Asp Ala
Asn 165 170 175 His Gly Ala Ala Ser Lys Lys Pro Trp Ile Asn Asn Leu
Leu Gly Leu 180 185 190 Gly Ala Asp Phe Ile Gly Gly Ser Lys Trp Leu
Trp Met Asn Gln His 195 200 205 Trp Thr His His Thr Tyr Thr Asn His
His Glu Lys Asp Pro Asp Ala 210 215 220 Leu Gly Ala Glu Pro Met Leu
Leu Phe Asn Asp Tyr Pro Leu Gly His 225 230 235 240 Pro Lys Arg Thr
Leu Ile His His Phe Gln Ala Phe Tyr Tyr Leu Phe 245 250 255 Val Leu
Ala Gly Tyr Trp Val Ser Ser Val Phe Asn Pro Gln Ile Leu 260 265 270
Asp Leu Gln His Arg Gly Ala Gln Ala Val Gly Met Lys Met Glu Asn 275
280 285 Asp Tyr Ile Ala Lys Ser Arg Lys Tyr Ala Ile Phe Leu Arg Leu
Leu 290 295 300 Tyr Ile Tyr Thr Asn Ile Val Ala Pro Ile Gln Asn Gln
Gly Phe Ser 305 310 315 320 Leu Thr Val Val Ala His Ile Leu Thr Met
Gly Val Ala Ser Ser Leu 325 330 335 Thr Leu Ala Thr Leu Phe Ala Leu
Ser His Asn Phe Glu Asn Ala Asp 340 345 350 Arg Asp Pro Thr Tyr Glu
Ala Arg Lys Gly Gly Glu Pro Val Cys Trp 355 360 365 Phe Lys Ser Gln
Val Glu Thr Ser Ser Thr Tyr Gly Gly Phe Ile Ser 370 375 380 Gly Cys
Leu Thr Gly Gly Leu Asn Phe Gln Val Glu His His Leu Phe 385 390 395
400 Pro Arg Met Ser Ser Ala Trp Tyr Pro Tyr Ile Ala Pro Thr Val Arg
405 410 415 Glu Val Cys Lys Lys His Gly Val Lys Tyr Ala Tyr Tyr Pro
Trp Val 420 425 430 Trp Gln Asn Leu Ile Ser Thr Val Lys Tyr Leu His
Gln Ser Gly Thr 435 440 445 Gly Ser Asn Trp Lys Asn Gly Ala Asn Pro
Tyr Ser Gly Lys Leu 450 455 460 <210> SEQ ID NO 79
<211> LENGTH: 1350 <212> TYPE: DNA <213>
ORGANISM: Euglena gracilis <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (1)..(1350)
<223> OTHER INFORMATION: delta-5 desaturase <400>
SEQUENCE: 79 atggctctca gtcttaccac agaacagctg ttagaacgcc ctgatttggt
tgcgattgat 60 ggcatcctct acgaccttga agggcttgcc aaagttcatc
caggaggaga tttgattctc 120 gcttctggtg cctctgatgc ctcccctctc
ttttattcaa tgcatccata cgtcaaaccg 180 gagaattcca aattgcttca
acagttcgtc cgagggaagc atgaccgcac ctcgaaggac 240 attgtctaca
cgtatgattc tcccttcgca caagacgtta agcggacaat gcgcgaggtg 300
atgaaaggga ggaactggta cgcaacccct ggcttctggc tgcgcaccgt tgggatcatc
360 gccgtgacgg ccttttgcga gtggcactgg gctaccacgg ggatggtgct
gtggggcctg 420 ttgactggat tcatgcacat gcagatcggc ttatccatcc
agcatgatgc gtcccacggg 480 gccatcagca agaagccttg ggtcaacgcc
ctcttcgcct acggcattga cgtcatcgga 540
tcgtcccggt ggatttggct gcagtcgcac atcatgcggc accacaccta caccaaccag
600 cacggcctcg acctggatgc ggagtcggca gagccgttcc tggtgttcca
caactacccc 660 gccgcaaaca ccgcccgaaa gtggttccac cgcttccaag
cttggtacat gtaccttgtg 720 ctgggggcat acggggtatc gctggtgtac
aacccgctct acattttccg gatgcagcac 780 aatgacacca tcccagagtc
tgtcacggcc atgcgggaga atggctttct gcggcgctac 840 cgcacacttg
cattcgtgat gcgagctttc ttcatcttcc ggaccgcatt cttgccctgg 900
tacctcactg ggacctcatt gctgatcacc attcctctgg tgcccactgc aactggtgcc
960 ttcttgacgt tcttcttcat tttgtcccac aattttgatg gctccgaacg
gatccccgac 1020 aagaactgca aggttaagag ctctgagaag gacgttgagg
ctgaccaaat tgactggtat 1080 cgggcgcagg tggagacgtc ctccacatac
ggtggcccca tcgccatgtt cttcactggc 1140 ggtctcaatt tccagatcga
gcaccacctc tttccccgga tgtcgtcttg gcactacccc 1200 ttcgtccagc
aggcggtccg ggagtgttgc gaacgccatg gagtgcgata tgttttctac 1260
cctaccatcg tcggcaacat catctccacc ctgaagtaca tgcataaggt gggtgtcgtc
1320 cactgcgtga aggacgcaca ggattcctga 1350 <210> SEQ ID NO 80
<211> LENGTH: 7222 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pFBAIn-MOD-1 <400> SEQUENCE: 80
catggatcca ggcctgttaa cggccattac ggcctgcagg atccgaaaaa acctcccaca
60 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
tgtttattgc 120 agcttataat ggttacaaat aaagcaatag catcacaaat
ttcacaaata aagcattttt 180 ttcactgcat tctagttgtg gtttgtccaa
actcatcaat gtatcttatc atgtctgcgg 240 ccgcaagtgt ggatggggaa
gtgagtgccc ggttctgtgt gcacaattgg caatccaaga 300 tggatggatt
caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg 360
atatttatgt ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa
420 catactgtac atactcatac tcgtacccgg gcaacggttt cacttgagtg
cagtggctag 480 tgctcttact cgtacagtgt gcaatactgc gtatcatagt
ctttgatgta tatcgtattc 540 attcatgtta gttgcgtacg agccggaagc
ataaagtgta aagcctgggg tgcctaatga 600 gtgagctaac tcacattaat
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 660 tcgtgccagc
tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 720
cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
780 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
taacgcagga 840 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
gtaaaaaggc cgcgttgctg 900 gcgtttttcc ataggctccg cccccctgac
gagcatcaca aaaatcgacg ctcaagtcag 960 aggtggcgaa acccgacagg
actataaaga taccaggcgt ttccccctgg aagctccctc 1020 gtgcgctctc
ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 1080
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1140 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
cgccttatcc 1200 ggtaactatc gtcttgagtc caacccggta agacacgact
tatcgccact ggcagcagcc 1260 actggtaaca ggattagcag agcgaggtat
gtaggcggtg ctacagagtt cttgaagtgg 1320 tggcctaact acggctacac
tagaaggaca gtatttggta tctgcgctct gctgaagcca 1380 gttaccttcg
gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1440
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
1500 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
ttaagggatt 1560 ttggtcatga gattatcaaa aaggatcttc acctagatcc
ttttaaatta aaaatgaagt 1620 tttaaatcaa tctaaagtat atatgagtaa
acttggtctg acagttacca atgcttaatc 1680 agtgaggcac ctatctcagc
gatctgtcta tttcgttcat ccatagttgc ctgactcccc 1740 gtcgtgtaga
taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 1800
ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
1860 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
taattgttgc 1920 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
gcaacgttgt tgccattgct 1980 acaggcatcg tggtgtcacg ctcgtcgttt
ggtatggctt cattcagctc cggttcccaa 2040 cgatcaaggc gagttacatg
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 2100 cctccgatcg
ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 2160
ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
2220 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
cccggcgtca 2280 atacgggata ataccgcgcc acatagcaga actttaaaag
tgctcatcat tggaaaacgt 2340 tcttcggggc gaaaactctc aaggatctta
ccgctgttga gatccagttc gatgtaaccc 2400 actcgtgcac ccaactgatc
ttcagcatct tttactttca ccagcgtttc tgggtgagca 2460 aaaacaggaa
ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 2520
ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
2580 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
cacatttccc 2640 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
gcgcggcggg tgtggtggtt 2700 acgcgcagcg tgaccgctac acttgccagc
gccctagcgc ccgctccttt cgctttcttc 2760 ccttcctttc tcgccacgtt
cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 2820 ttagggttcc
gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 2880
ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
2940 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
tatctcggtc 3000 tattcttttg atttataagg gattttgccg atttcggcct
attggttaaa aaatgagctg 3060 atttaacaaa aatttaacgc gaattttaac
aaaatattaa cgcttacaat ttccattcgc 3120 cattcaggct gcgcaactgt
tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 3180 agctggcgaa
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 3240
agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
3300 tgggtaccgg gccccccctc gaggtcgatg gtgtcgataa gcttgatatc
gaattcatgt 3360 cacacaaacc gatcttcgcc tcaaggaaac ctaattctac
atccgagaga ctgccgagat 3420 ccagtctaca ctgattaatt ttcgggccaa
taatttaaaa aaatcgtgtt atataatatt 3480 atatgtatta tatatataca
tcatgatgat actgacagtc atgtcccatt gctaaataga 3540 cagactccat
ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg 3600
tttaataata aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat
3660 gaacttattt ttattactta gtattattag acaacttact tgctttatga
aaaacacttc 3720 ctatttagga aacaatttat aatggcagtt cgttcattta
acaatttatg tagaataaat 3780 gttataaatg cgtatgggaa atcttaaata
tggatagcat aaatgatatc tgcattgcct 3840 aattcgaaat caacagcaac
gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa 3900 tatcaactat
caaagaacag ctattcacac gttactattg agattattat tggacgagaa 3960
tcacacactc aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc
4020 attgttcata cttctagtca tttcatccca catattcctt ggatttctct
ccaatgaatg 4080 acattctatc ttgcaaattc aacaattata ataagatata
ccaaagtagc ggtatagtgg 4140 caatcaaaaa gcttctctgg tgtgcttctc
gtatttattt ttattctaat gatccattaa 4200 aggtatatat ttatttcttg
ttatataatc cttttgttta ttacatgggc tggatacata 4260 aaggtatttt
gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt 4320
aatggtagga aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg
4380 tatttccagg ttagacgttc cgcagaatct agaatgcggt atgcggtaca
ttgttcttcg 4440 aacgtaaaag ttgcgctccc tgagatattg tacatttttg
cttttacaag tacaagtaca 4500 tcgtacaact atgtactact gttgatgcat
ccacaacagt ttgttttgtt tttttttgtt 4560 tttttttttt ctaatgattc
attaccgcta tgtataccta cttgtacttg tagtaagccg 4620 ggttattggc
gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta 4680
cttttagctt atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat
4740 gctcaatcga tttcgacagt aattaattaa gtcatacaca agtcagcttt
cttcgagcct 4800 catataagta taagtagttc aacgtattag cactgtaccc
agcatctccg tatcgagaaa 4860 cacaacaaca tgccccattg gacagatcat
gcggatacac aggttgtgca gtatcataca 4920 tactcgatca gacaggtcgt
ctgaccatca tacaagctga acaagcgctc catacttgca 4980 cgctctctat
atacacagtt aaattacata tccatagtct aacctctaac agttaatctt 5040
ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
5100 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca
tgacatcctc 5160 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg
tcaagaccca ccccgggggt 5220 cagaataagc cagtcctcag agtcgccctt
aggtcggttc tgggcaatga agccaaccac 5280 aaactcgggg tcggatcggg
caagctcaat ggtctgcttg gagtactcgc cagtggccag 5340 agagcccttg
caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg 5400
agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
5460 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc
cggttccggg 5520 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt
cggtgacacc ggtactggtg 5580 cttgacagtg ttgccaatat ctgcgaactt
tctgtcctcg aacaggaaga aaccgtgctt 5640 aagagcaagt tccttgaggg
ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc 5700 gatatgggtt
ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc 5760
cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt
5820 gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt
aggagggcat 5880 tttggtggtg aagaggagac tgaaataaat ttagtctgca
gaacttttta tcggaacctt 5940 atctggggca gtgaagtata tgttatggta
atagttacga gttagttgaa cttatagata 6000 gactggacta tacggctatc
ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc 6060 gcctttgccg
acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt 6120
gttgtcggcc aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg
6180 tatcgtcaaa gtgatccaag cacactcata gttggagtcg tactccaaag
gcggcaatga 6240 cgagtcagac agatactcgt cgaaaacagt gtacgcagat
ctactataga ggaacattta 6300 aattgccccg gagaagacgg ccaggccgcc
tagatgacaa attcaacaac tcacagctga 6360
ctttctgcca ttgccactag gggggggcct ttttatatgg ccaagccaag ctctccacgt
6420 cggttgggct gcacccaaca ataaatgggt agggttgcac caacaaaggg
atgggatggg 6480 gggtagaaga tacgaggata acggggctca atggcacaaa
taagaacgaa tactgccatt 6540 aagactcgtg atccagcgac tgacaccatt
gcatcatcta agggcctcaa aactacctcg 6600 gaactgctgc gctgatctgg
acaccacaga ggttccgagc actttaggtt gcaccaaatg 6660 tcccaccagg
tgcaggcaga aaacgctgga acagcgtgta cagtttgtct taacaaaaag 6720
tgagggcgct gaggtcgagc agggtggtgt gacttgttat agcctttaga gctgcgaaag
6780 cgcgtatgga tttggctcat caggccagat tgagggtctg tggacacatg
tcatgttagt 6840 gtacttcaat cgccccctgg atatagcccc gacaataggc
cgtggcctca tttttttgcc 6900 ttccgcacat ttccattgct cggtacccac
accttgcttc tcctgcactt gccaacctta 6960 atactggttt acattgacca
acatcttaca agcggggggc ttgtctaggg tatatataaa 7020 cagtggctct
cccaatcggt tgccagtctc ttttttcctt tctttcccca cagattcgaa 7080
atctaaacta cacatcacag aattccgagc cgtgagtatc cacgacaaga tcagtgtcga
7140 gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct agcaacacac
actctctaca 7200 caaactaacc cagctctggt ac 7222 <210> SEQ ID NO
81 <211> LENGTH: 1086 <212> TYPE: DNA <213>
ORGANISM: Phytophthora sojae <220> FEATURE: <221>
NAME/KEY: misc_feature <223> OTHER INFORMATION: synthetic
delta-17 desaturase (codon-optimized) <400> SEQUENCE: 81
atggctacca agcagcccta ccagttccct actctgaccg agatcaagcg atctctgccc
60 tccgagtgtt tcgaggcctc cgtgcctctc tctctgtact acaccgttcg
atgcctggtc 120 attgctgtgt cgctcgcctt cggacttcac catgcacgat
ctctgcccgt tgtcgaaggc 180 ctctgggctc tggatgccgc tctctgcacc
ggttacgtgc tgctccaggg catcgtcttc 240 tggggattct ttactgttgg
tcacgacgct ggacatggtg ccttctcccg ataccacctg 300 ctcaactttg
tcatcggaac cttcattcac tctctcatcc ttacaccctt cgagtcctgg 360
aagctcaccc acagacacca tcacaagaac actggcaaca tcgaccgaga cgaaatcttc
420 taccctcaac gaaaggccga cgatcatcct ctgtctcgaa acctcattct
ggctttgggt 480 gcagcctggt ttgcctacct ggtcgaaggc tttcctcccc
gaaaggtcaa ccacttcaac 540 cccttcgagc ctctctttgt tcgacaggtc
tctgccgtgg tcatttcgct ggctgcgcac 600 tttggagtgg ctgccctgtc
catctacctc agcctgcagt tcggcttcaa gactatggcc 660 atctactact
atggtcccgt ctttgtgttc ggatccatgc tcgtcattac tacctttctt 720
catcacaacg acgaagagac accttggtac gcagattcgg agtggaccta cgtcaaaggc
780 aacctgtcct ctgtcgaccg atcctacggt gccctcatcg acaacctttc
tcacaacatc 840 ggaacccacc agattcatca cctctttccc atcattcctc
actacaagct caagcgagct 900 accgaggcct tccatcaagc ctttcccgag
ctggttcgaa agtccgacga acccatcatc 960 aaggcctttt tcagagtcgg
ccgactctac gcaaactacg gtgtggtcga ctcggatgcc 1020 aagctgttca
ctctcaagga ggccaaggct gtttccgaag ccgctaccaa gactaaggcc 1080 acctaa
1086 <210> SEQ ID NO 82 <211> LENGTH: 361 <212>
TYPE: PRT <213> ORGANISM: Phytophthora sojae <220>
FEATURE: <221> NAME/KEY: MISC_FEATURE <223> OTHER
INFORMATION: synthetic delta-17 desaturase (codon-optimized)
<400> SEQUENCE: 82 Met Ala Thr Lys Gln Pro Tyr Gln Phe Pro
Thr Leu Thr Glu Ile Lys 1 5 10 15 Arg Ser Leu Pro Ser Glu Cys Phe
Glu Ala Ser Val Pro Leu Ser Leu 20 25 30 Tyr Tyr Thr Val Arg Cys
Leu Val Ile Ala Val Ser Leu Ala Phe Gly 35 40 45 Leu His His Ala
Arg Ser Leu Pro Val Val Glu Gly Leu Trp Ala Leu 50 55 60 Asp Ala
Ala Leu Cys Thr Gly Tyr Val Leu Leu Gln Gly Ile Val Phe 65 70 75 80
Trp Gly Phe Phe Thr Val Gly His Asp Ala Gly His Gly Ala Phe Ser 85
90 95 Arg Tyr His Leu Leu Asn Phe Val Ile Gly Thr Phe Ile His Ser
Leu 100 105 110 Ile Leu Thr Pro Phe Glu Ser Trp Lys Leu Thr His Arg
His His His 115 120 125 Lys Asn Thr Gly Asn Ile Asp Arg Asp Glu Ile
Phe Tyr Pro Gln Arg 130 135 140 Lys Ala Asp Asp His Pro Leu Ser Arg
Asn Leu Ile Leu Ala Leu Gly 145 150 155 160 Ala Ala Trp Phe Ala Tyr
Leu Val Glu Gly Phe Pro Pro Arg Lys Val 165 170 175 Asn His Phe Asn
Pro Phe Glu Pro Leu Phe Val Arg Gln Val Ser Ala 180 185 190 Val Val
Ile Ser Leu Ala Ala His Phe Gly Val Ala Ala Leu Ser Ile 195 200 205
Tyr Leu Ser Leu Gln Phe Gly Phe Lys Thr Met Ala Ile Tyr Tyr Tyr 210
215 220 Gly Pro Val Phe Val Phe Gly Ser Met Leu Val Ile Thr Thr Phe
Leu 225 230 235 240 His His Asn Asp Glu Glu Thr Pro Trp Tyr Ala Asp
Ser Glu Trp Thr 245 250 255 Tyr Val Lys Gly Asn Leu Ser Ser Val Asp
Arg Ser Tyr Gly Ala Leu 260 265 270 Ile Asp Asn Leu Ser His Asn Ile
Gly Thr His Gln Ile His His Leu 275 280 285 Phe Pro Ile Ile Pro His
Tyr Lys Leu Lys Arg Ala Thr Glu Ala Phe 290 295 300 His Gln Ala Phe
Pro Glu Leu Val Arg Lys Ser Asp Glu Pro Ile Ile 305 310 315 320 Lys
Ala Phe Phe Arg Val Gly Arg Leu Tyr Ala Asn Tyr Gly Val Val 325 330
335 Asp Ser Asp Ala Lys Leu Phe Thr Leu Lys Glu Ala Lys Ala Val Ser
340 345 350 Glu Ala Ala Thr Lys Thr Lys Ala Thr 355 360 <210>
SEQ ID NO 83 <211> LENGTH: 3806 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pPsD17S <400>
SEQUENCE: 83 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt
aatcatggtc 60 atagctgttt cctgtgtgaa attgttatcc gctcacaatt
ccacacaaca tacgagccgg 120 aagcataaag tgtaaagcct ggggtgccta
atgagtgagc taactcacat taattgcgtt 180 gcgctcactg cccgctttcc
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 240 ccaacgcgcg
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 300
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
360 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
aaggccagca 420 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
ttccataggc tccgcccccc 480 tgacgagcat cacaaaaatc gacgctcaag
tcagaggtgg cgaaacccga caggactata 540 aagataccag gcgtttcccc
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 600 gcttaccgga
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 660
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
720 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
agtccaaccc 780 ggtaagacac gacttatcgc cactggcagc agccactggt
aacaggatta gcagagcgag 840 gtatgtaggc ggtgctacag agttcttgaa
gtggtggcct aactacggct acactagaag 900 aacagtattt ggtatctgcg
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 960 ctcttgatcc
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 1020
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
1080 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
caaaaaggat 1140 cttcacctag atccttttaa attaaaaatg aagttttaaa
tcaatctaaa gtatatatga 1200 gtaaacttgg tctgacagtt accaatgctt
aatcagtgag gcacctatct cagcgatctg 1260 tctatttcgt tcatccatag
ttgcctgact ccccgtcgtg tagataacta cgatacggga 1320 gggcttacca
tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 1380
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
1440 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
gtagttcgcc 1500 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
atcgtggtgt cacgctcgtc 1560 gtttggtatg gcttcattca gctccggttc
ccaacgatca aggcgagtta catgatcccc 1620 catgttgtgc aaaaaagcgg
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 1680 ggccgcagtg
ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 1740
atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
1800 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
cgccacatag 1860 cagaacttta aaagtgctca tcattggaaa acgttcttcg
gggcgaaaac tctcaaggat 1920 cttaccgctg ttgagatcca gttcgatgta
acccactcgt gcacccaact gatcttcagc 1980 atcttttact ttcaccagcg
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 2040 aaagggaata
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 2100
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
2160 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
acgtctaaga 2220 aaccattatt atcatgacat taacctataa aaataggcgt
atcacgaggc cctttcgtct 2280 cgcgcgtttc ggtgatgacg gtgaaaacct
ctgacacatg cagctcccgg agacggtcac 2340 agcttgtctg taagcggatg
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 2400
tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
2460 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
tcaggcgcca 2520 ttcgccattc aggctgcgca actgttggga agggcgatcg
gtgcgggcct cttcgctatt 2580 acgccagctg gcgaaagggg gatgtgctgc
aaggcgatta agttgggtaa cgccagggtt 2640 ttcccagtca cgacgttgta
aaacgacggc cagtgaattc gagctcggta cctcgcgaat 2700 gcatctagat
ccatggctac caagcagccc taccagttcc ctactctgac cgagatcaag 2760
cgatctctgc cctccgagtg tttcgaggcc tccgtgcctc tctctctgta ctacaccgtt
2820 cgatgcctgg tcattgctgt gtcgctcgcc ttcggacttc accatgcacg
atctctgccc 2880 gttgtcgaag gcctctgggc tctggatgcc gctctctgca
ccggttacgt gctgctccag 2940 ggcatcgtct tctggggatt ctttactgtt
ggtcacgacg ctggacatgg tgccttctcc 3000 cgataccacc tgctcaactt
tgtcatcgga accttcattc actctctcat ccttacaccc 3060 ttcgagtcct
ggaagctcac ccacagacac catcacaaga acactggcaa catcgaccga 3120
gacgaaatct tctaccctca acgaaaggcc gacgatcatc ctctgtctcg aaacctcatt
3180 ctggctttgg gtgcagcctg gtttgcctac ctggtcgaag gctttcctcc
ccgaaaggtc 3240 aaccacttca accccttcga gcctctcttt gttcgacagg
tctctgccgt ggtcatttcg 3300 ctggctgcgc actttggagt ggctgccctg
tccatctacc tcagcctgca gttcggcttc 3360 aagactatgg ccatctacta
ctatggtccc gtctttgtgt tcggatccat gctcgtcatt 3420 actacctttc
ttcatcacaa cgacgaagag acaccttggt acgcagattc ggagtggacc 3480
tacgtcaaag gcaacctgtc ctctgtcgac cgatcctacg gtgccctcat cgacaacctt
3540 tctcacaaca tcggaaccca ccagattcat cacctctttc ccatcattcc
tcactacaag 3600 ctcaagcgag ctaccgaggc cttccatcaa gcctttcccg
agctggttcg aaagtccgac 3660 gaacccatca tcaaggcctt tttcagagtc
ggccgactct acgcaaacta cggtgtggtc 3720 gactcggatg ccaagctgtt
cactctcaag gaggccaagg ctgtttccga agccgctacc 3780 aagactaagg
ccacctaagc ggccgc 3806 <210> SEQ ID NO 84 <211> LENGTH:
1086 <212> TYPE: DNA <213> ORGANISM: Phytophthora
ramorum <220> FEATURE: <221> NAME/KEY: misc_feature
<223> OTHER INFORMATION: synthetic delta-17 desaturase
(codon-optimized) <400> SEQUENCE: 84 atggctacca agcagcccta
ccagttccct actctgaccg agatcaagcg atctcttccc 60 tccgagtgct
ttgaagcctc ggtccctctg tccttgtact acaccgtgcg aatcgtcgct 120
attgccgttg ctctggcctt cggactcaac tacgctcgag cccttcccgt ggtcgagtct
180 ctgtgggcac tcgacgctgc cctttgttgc ggttacgttc tgctccaagg
cattgtcttc 240 tggggattct ttaccgtggg tcacgatgct ggacatggtg
ccttctctcg ataccacctg 300 ctcaactttg tcgttggcac ctttatccac
tccctcattc ttactccctt cgagtcgtgg 360 aagctcacac atcgacacca
tcacaagaac accggaaaca tcgaccgaga cgaaatcttc 420 taccctcagc
gaaaggccga cgatcatcct ctgtctcgaa acctcgtcct ggctctcggt 480
gccgcttggt ttgcctacct tgtcgagggc tttcctcccc gaaaggtcaa ccacttcaac
540 cccttcgaac ctctgtttgt gcgacaggtg gctgccgttg tcatttccct
ctctgctcac 600 ttcgccgtcc tggcactgtc cgtgtatctg agctttcagt
tcggtctcaa gacaatggct 660 ctgtactact atggacccgt cttcgtgttc
ggctccatgc tcgtcattac tacctttctg 720 catcacaatg acgaggaaac
tccttggtac ggagattccg actggaccta cgtcaagggc 780 aacttgtctt
ccgtggaccg atcttacggt gccttcatcg acaacctctc gcacaacatt 840
ggcacacacc agatccacca tctgtttccc atcattcctc actacaagct caaccgagcc
900 accgctgcct tccaccaggc ctttcccgaa cttgtccgaa agagcgacga
gcccattctc 960 aaggctttct ggagagttgg tcgactttac gccaactacg
gagtcgtgga tcccgacgca 1020 aagctgttta ctctcaagga ggccaaagct
gcctccgagg ctgccaccaa gaccaaggct 1080 acttaa 1086 <210> SEQ
ID NO 85 <211> LENGTH: 3806 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pPrD17S <400> SEQUENCE: 85
ggccgcatcg gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc
60 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
acaacatacg 120 agccggaagc ataaagtgta aagcctgggg tgcctaatga
gtgagctaac tcacattaat 180 tgcgttgcgc tcactgcccg ctttccagtc
gggaaacctg tcgtgccagc tgcattaatg 240 aatcggccaa cgcgcgggga
gaggcggttt gcgtattggg cgctcttccg cttcctcgct 300 cactgactcg
ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 360
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
420 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
ataggctccg 480 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
aggtggcgaa acccgacagg 540 actataaaga taccaggcgt ttccccctgg
aagctccctc gtgcgctctc ctgttccgac 600 cctgccgctt accggatacc
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 660 tagctcacgc
tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 720
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
780 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
ggattagcag 840 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
tggcctaact acggctacac 900 tagaagaaca gtatttggta tctgcgctct
gctgaagcca gttaccttcg gaaaaagagt 960 tggtagctct tgatccggca
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1020 gcagcagatt
acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1080
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
1140 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
tctaaagtat 1200 atatgagtaa acttggtctg acagttacca atgcttaatc
agtgaggcac ctatctcagc 1260 gatctgtcta tttcgttcat ccatagttgc
ctgactcccc gtcgtgtaga taactacgat 1320 acgggagggc ttaccatctg
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1380 ggctccagat
ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 1440
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
1500 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
tggtgtcacg 1560 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
cgatcaaggc gagttacatg 1620 atcccccatg ttgtgcaaaa aagcggttag
ctccttcggt cctccgatcg ttgtcagaag 1680 taagttggcc gcagtgttat
cactcatggt tatggcagca ctgcataatt ctcttactgt 1740 catgccatcc
gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 1800
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
1860 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
gaaaactctc 1920 aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc 1980 ttcagcatct tttactttca ccagcgtttc
tgggtgagca aaaacaggaa ggcaaaatgc 2040 cgcaaaaaag ggaataaggg
cgacacggaa atgttgaata ctcatactct tcctttttca 2100 atattattga
agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2160
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
2220 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
cgaggccctt 2280 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
cacatgcagc tcccggagac 2340 ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc 2400 gggtgttggc gggtgtcggg
gctggcttaa ctatgcggca tcagagcaga ttgtactgag 2460 agtgcaccat
atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 2520
gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
2580 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
gggtaacgcc 2640 agggttttcc cagtcacgac gttgtaaaac gacggccagt
gaattcgagc tcggtacctc 2700 gcgaatgcat ctagatccat ggctaccaag
cagccctacc agttccctac tctgaccgag 2760 atcaagcgat ctcttccctc
cgagtgcttt gaagcctcgg tccctctgtc cttgtactac 2820 accgtgcgaa
tcgtcgctat tgccgttgct ctggccttcg gactcaacta cgctcgagcc 2880
cttcccgtgg tcgagtctct gtgggcactc gacgctgccc tttgttgcgg ttacgttctg
2940 ctccaaggca ttgtcttctg gggattcttt accgtgggtc acgatgctgg
acatggtgcc 3000 ttctctcgat accacctgct caactttgtc gttggcacct
ttatccactc cctcattctt 3060 actcccttcg agtcgtggaa gctcacacat
cgacaccatc acaagaacac cggaaacatc 3120 gaccgagacg aaatcttcta
ccctcagcga aaggccgacg atcatcctct gtctcgaaac 3180 ctcgtcctgg
ctctcggtgc cgcttggttt gcctaccttg tcgagggctt tcctccccga 3240
aaggtcaacc acttcaaccc cttcgaacct ctgtttgtgc gacaggtggc tgccgttgtc
3300 atttccctct ctgctcactt cgccgtcctg gcactgtccg tgtatctgag
ctttcagttc 3360 ggtctcaaga caatggctct gtactactat ggacccgtct
tcgtgttcgg ctccatgctc 3420 gtcattacta cctttctgca tcacaatgac
gaggaaactc cttggtacgg agattccgac 3480 tggacctacg tcaagggcaa
cttgtcttcc gtggaccgat cttacggtgc cttcatcgac 3540 aacctctcgc
acaacattgg cacacaccag atccaccatc tgtttcccat cattcctcac 3600
tacaagctca accgagccac cgctgccttc caccaggcct ttcccgaact tgtccgaaag
3660 agcgacgagc ccattctcaa ggctttctgg agagttggtc gactttacgc
caactacgga 3720 gtcgtggatc ccgacgcaaa gctgtttact ctcaaggagg
ccaaagctgc ctccgaggct 3780 gccaccaaga ccaaggctac ttaagc 3806
<210> SEQ ID NO 86 <211> LENGTH: 1209 <212> TYPE:
DNA <213> ORGANISM: Fusarium monoliforme <300>
PUBLICATION INFORMATION: <302> TITLE: DELTA-15 DESATURASES
SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY ACIDS IN
OLEAGINOUS PLANTS AND YEAST <310> PATENT DOCUMENT NUMBER: WO
2005/047480 <311> PATENT FILING DATE: 2004-11-10 <312>
PUBLICATION DATE: 2005-05-26 <313> RELEVANT RESIDUES IN SEQ
ID NO: (1)..(1209) <400> SEQUENCE: 86
atggcgactc gacagcgaac tgccaccact gttgtggtcg aggaccttcc caaggtcact
60 cttgaggcca agtctgaacc tgtgttcccc gatatcaaga ccatcaagga
tgccattccc 120 gcgcactgct tccagccctc gctcgtcacc tcattctact
acgtcttccg cgattttgct 180 atggtctctg ccctcgtctg ggctgctctc
acctacatcc ccagcatccc cgaccagacc 240 ctccgcgtcg cagcttggat
ggtctacggc ttcgtccagg gtctgttctg caccggtgtc 300 tggattctcg
gccatgagtg cggccacggt gctttctctc tccacggaaa ggtcaacaat 360
gtgaccggct ggttcctcca ctcgttcctc ctcgtcccct acttcagctg gaagtactct
420 caccaccgcc accaccgctt caccggccac atggatctcg acatggcttt
cgtccccaag 480 actgagccca agccctccaa gtcgctcatg attgctggca
ttgacgtcgc cgagcttgtt 540 gaggacaccc ccgctgctca gatggtcaag
ctcatcttcc accagctttt cggatggcag 600 gcgtacctct tcttcaacgc
tagctctggc aagggcagca agcagtggga gcccaagact 660 ggcctctcca
agtggttccg agtcagtcac ttcgagccta ccagcgctgt cttccgcccc 720
aacgaggcca tcttcatcct catctccgat atcggtcttg ctctaatggg aactgctctg
780 tactttgctt ccaagcaagt tggtgtttcg accattctct tcctctacct
tgttccctac 840 ctgtgggttc accactggct cgttgccatt acctacctcc
accaccacca caccgagctc 900 cctcactaca ccgctgaggg ctggacctac
gtcaagggag ctctcgccac tgtcgaccgt 960 gagtttggct tcatcggaaa
gcacctcttc cacggtatca ttgagaagca cgttgttcac 1020 catctcttcc
ctaagatccc cttctacaag gctgacgagg ccaccgaggc catcaagccc 1080
gtcattggcg accactactg ccacgacgac cgaagcttcc tgggccagct gtggaccatc
1140 ttcggcacgc tcaagtacgt cgagcacgac cctgcccgac ccggtgccat
gcgatggaac 1200 aaggactag 1209 <210> SEQ ID NO 87 <211>
LENGTH: 402 <212> TYPE: PRT <213> ORGANISM: Fusarium
monoliforme <300> PUBLICATION INFORMATION: <302> TITLE:
DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF
POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST
<310> PATENT DOCUMENT NUMBER: WO 2005/047480 <311>
PATENT FILING DATE: 2004-11-10 <312> PUBLICATION DATE:
2005-05-26 <313> RELEVANT RESIDUES IN SEQ ID NO: (1)..(402)
<400> SEQUENCE: 87 Met Ala Thr Arg Gln Arg Thr Ala Thr Thr
Val Val Val Glu Asp Leu 1 5 10 15 Pro Lys Val Thr Leu Glu Ala Lys
Ser Glu Pro Val Phe Pro Asp Ile 20 25 30 Lys Thr Ile Lys Asp Ala
Ile Pro Ala His Cys Phe Gln Pro Ser Leu 35 40 45 Val Thr Ser Phe
Tyr Tyr Val Phe Arg Asp Phe Ala Met Val Ser Ala 50 55 60 Leu Val
Trp Ala Ala Leu Thr Tyr Ile Pro Ser Ile Pro Asp Gln Thr 65 70 75 80
Leu Arg Val Ala Ala Trp Met Val Tyr Gly Phe Val Gln Gly Leu Phe 85
90 95 Cys Thr Gly Val Trp Ile Leu Gly His Glu Cys Gly His Gly Ala
Phe 100 105 110 Ser Leu His Gly Lys Val Asn Asn Val Thr Gly Trp Phe
Leu His Ser 115 120 125 Phe Leu Leu Val Pro Tyr Phe Ser Trp Lys Tyr
Ser His His Arg His 130 135 140 His Arg Phe Thr Gly His Met Asp Leu
Asp Met Ala Phe Val Pro Lys 145 150 155 160 Thr Glu Pro Lys Pro Ser
Lys Ser Leu Met Ile Ala Gly Ile Asp Val 165 170 175 Ala Glu Leu Val
Glu Asp Thr Pro Ala Ala Gln Met Val Lys Leu Ile 180 185 190 Phe His
Gln Leu Phe Gly Trp Gln Ala Tyr Leu Phe Phe Asn Ala Ser 195 200 205
Ser Gly Lys Gly Ser Lys Gln Trp Glu Pro Lys Thr Gly Leu Ser Lys 210
215 220 Trp Phe Arg Val Ser His Phe Glu Pro Thr Ser Ala Val Phe Arg
Pro 225 230 235 240 Asn Glu Ala Ile Phe Ile Leu Ile Ser Asp Ile Gly
Leu Ala Leu Met 245 250 255 Gly Thr Ala Leu Tyr Phe Ala Ser Lys Gln
Val Gly Val Ser Thr Ile 260 265 270 Leu Phe Leu Tyr Leu Val Pro Tyr
Leu Trp Val His His Trp Leu Val 275 280 285 Ala Ile Thr Tyr Leu His
His His His Thr Glu Leu Pro His Tyr Thr 290 295 300 Ala Glu Gly Trp
Thr Tyr Val Lys Gly Ala Leu Ala Thr Val Asp Arg 305 310 315 320 Glu
Phe Gly Phe Ile Gly Lys His Leu Phe His Gly Ile Ile Glu Lys 325 330
335 His Val Val His His Leu Phe Pro Lys Ile Pro Phe Tyr Lys Ala Asp
340 345 350 Glu Ala Thr Glu Ala Ile Lys Pro Val Ile Gly Asp His Tyr
Cys His 355 360 365 Asp Asp Arg Ser Phe Leu Gly Gln Leu Trp Thr Ile
Phe Gly Thr Leu 370 375 380 Lys Tyr Val Glu His Asp Pro Ala Arg Pro
Gly Ala Met Arg Trp Asn 385 390 395 400 Lys Asp <210> SEQ ID
NO 88 <211> LENGTH: 7668 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pY6.GPD.Leu2 <400> SEQUENCE: 88
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg ctcggtcgtt
cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca 300 aaaggccagc
aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 360
ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
420 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660 gagtccaacc
cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 720
agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
780 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg cagaaaaaaa
ggatctcaag aagatccttt gatcttttct 960 acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta 1020 tcaaaaagga
tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 1080
agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
gtagataact 1200 acgatacggg agggcttacc atctggcccc agtgctgcaa
tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc agcaataaac
cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa ctttatccgc
ctccatccag tctattaatt gttgccggga agctagagta 1380 agtagttcgc
cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1440
tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc atggttatgg
cagcactgca taattctctt 1620 actgtcatgc catccgtaag atgcttttct
gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740 gcgccacata
gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1800
ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
1860 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
gaatactcat actcttcctt 1980 tttcaatatt attgaagcat ttatcagggt
tattgtctca tgagcggata catatttgaa 2040 tgtatttaga aaaataaaca
aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100 gacgcgccct
gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2160
gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa cttgattagg
gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt tccaaactgg
aacaacactc aaccctatct cggtctattc ttttgattta 2460 taagggattt
tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 2520
aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg taatacgact
cactataggg cgaattgggt accgggcccc 2760 ccctcgaggt cgatggtgtc
gataagcttg atatcgaatt catgtcacac aaaccgatct 2820 tcgcctcaag
gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat 2880
taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat
2940 atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac
tccatctgcc 3000 gcctccaact gatgttctca atatttaagg ggtcatctcg
cattgtttaa taataaacag 3060 actccatcta ccgcctccaa atgatgttct
caaaatatat tgtatgaact tatttttatt 3120 acttagtatt attagacaac
ttacttgctt tatgaaaaac acttcctatt taggaaacaa 3180 tttataatgg
cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat 3240
gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca
3300 gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca
actatcaaag 3360 aacagctatt cacacgttac tattgagatt attattggac
gagaatcaca cactcaactg 3420 tctttctctc ttctagaaat acaggtacaa
gtatgtacta ttctcattgt tcatacttct 3480 agtcatttca tcccacatat
tccttggatt tctctccaat gaatgacatt ctatcttgca 3540 aattcaacaa
ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc 3600
tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt
3660 tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt
attttgattt 3720 aattttttgc ttaaattcaa tcccccctcg ttcagtgtca
actgtaatgg taggaaatta 3780 ccatactttt gaagaagcaa aaaaaatgaa
agaaaaaaaa aatcgtattt ccaggttaga 3840 cgttccgcag aatctagaat
gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg 3900 ctccctgaga
tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta 3960
ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat
4020 gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta
ttggcgttca 4080 attaatcata gacttatgaa tctgcacggt gtgcgctgcg
agttactttt agcttatgca 4140 tgctacttgg gtgtaatatt gggatctgtt
cggaaatcaa cggatgctca accgatttcg 4200 acagtaatta attaatttga
atcgaatcgg agcctaaaat gaacccgagt atatctcata 4260 aaattctcgg
tgagaggtct gtgactgtca gtacaaggtg ccttcattat gccctcaacc 4320
ttaccatacc tcactgaatg tagtgtacct ctaaaaatga aatacagtgc caaaagccaa
4380 ggcactgagc tcgtctaacg gacttgatat acaaccaatt aaaacaaatg
aaaagaaata 4440 cagttctttg tatcatttgt aacaattacc ctgtacaaac
taaggtattg aaatcccaca 4500 atattcccaa agtccacccc tttccaaatt
gtcatgccta caactcatat accaagcact 4560 aacctaccaa acaccactaa
aaccccacaa aatatatctt accgaatata cagtaacaag 4620 ctaccaccac
actcgttggg tgcagtcgcc agcttaaaga tatctatcca catcagccac 4680
aactcccttc ctttaataaa ccgactacac ccttggctat tgaggttatg agtgaatata
4740 ctgtagacaa gacactttca agaagactgt ttccaaaacg taccactgtc
ctccactaca 4800 aacacaccca atctgcttct tctagtcaag gttgctacac
cggtaaatta taaatcatca 4860 tttcattagc agggcagggc cctttttata
gagtcttata cactagcgga ccctgccggt 4920 agaccaaccc gcaggcgcgt
cagtttgctc cttccatcaa tgcgtcgtag aaacgactta 4980 ctccttcttg
agcagctcct tgaccttgtt ggcaacaagt ctccgacctc ggaggtggag 5040
gaagagcctc cgatatcggc ggtagtgata ccagcctcga cggactcctt gacggcagcc
5100 tcaacagcgt caccggcggg cttcatgtta agagagaact tgagcatcat
ggcggcagac 5160 agaatggtgg caatggggtt gaccttctgc ttgccgagat
cgggggcaga tccgtgacag 5220 ggctcgtaca gaccgaacgc ctcgttggtg
tcgggcagag aagccagaga ggcggagggc 5280 agcagaccca gagaaccggg
gatgacggag gcctcgtcgg agatgatatc gccaaacatg 5340 ttggtggtga
tgatgatacc attcatcttg gagggctgct tgatgaggat catggcggcc 5400
gagtcgatca gctggtggtt gagctcgagc tgggggaatt cgtccttgag gactcgagtg
5460 acagtctttc gccaaagtcg agaggaggcc agcacgttgg ccttgtcaag
agaccacacg 5520 ggaagagggg ggttgtgctg aagggccagg aaggcggcca
ttcgggcaat tcgctcaacc 5580 tcaggaacgg agtaggtctc ggtgtcggaa
gcgacgccag atccgtcatc ctcctttcgc 5640 tctccaaagt agatacctcc
gacgagctct cggacaatga tgaagtcggt gccctcaacg 5700 tttcggatgg
gggagagatc ggcgagcttg ggcgacagca gctggcaggg tcgcaggttg 5760
gcgtacaggt tcaggtcctt tcgcagcttg aggagaccct gctcgggtcg cacgtcggtt
5820 cgtccgtcgg gagtggtcca tacggtgttg gcagcgcctc cgacagcacc
gagcataata 5880 gagtcagcct ttcggcagat gtcgagagta gcgtcggtga
tgggctcgcc ctccttctca 5940 atggcagctc ctccaatgag tcggtcctca
aacacaaact cggtgccgga ggcctcagca 6000 acagacttga gcaccttgac
ggcctcggca atcacctcgg ggccacagaa gtcgccgccg 6060 agaagaacaa
tcttcttgga gtcagtcttg gtcttcttag tttcgggttc cattgtggat 6120
gtgtgtggtt gtatgtgtga tgtggtgtgt ggagtgaaaa tctgtggctg gcaaacgctc
6180 ttgtatatat acgcactttt gcccgtgcta tgtggaagac taaacctccg
aagattgtga 6240 ctcaggtagt gcggtatcgg ctagggaccc aaaccttgtc
gatgccgata gcgctatcga 6300 acgtacccca gccggccggg agtatgtcgg
aggggacata cgagatcgtc aagggtttgt 6360 ggccaactgg taaataaatg
atgtcgacgc agtaggatgt cctgcacggg tctttttgtg 6420 gggtgtggag
aaaggggtgc ttggagatgg aagccggtag aaccgggctg cttgtgcttg 6480
gagatggaag ccggtagaac cgggctgctt ggggggattt ggggccgctg ggctccaaag
6540 aggggtaggc atttcgttgg ggttacgtaa ttgcggcatt tgggtcctgc
gcgcatgtcc 6600 cattggtcag aattagtccg gataggagac ttatcagcca
atcacagcgc cggatccacc 6660 tgtaggttgg gttgggtggg agcacccctc
cacagagtag agtcaaacag cagcagcaac 6720 atgatagttg ggggtgtgcg
tgttaaagga aaaaaaagaa gcttgggtta tattcccgct 6780 ctatttagag
gttgcgggat agacgccgac ggagggcaat ggcgctatgg aaccttgcgg 6840
atatccatac gccgcggcgg actgcgtccg aaccagctcc agcagcgttt tttccgggcc
6900 attgagccga ctgcgacccc gccaacgtgt cttggcccac gcactcatgt
catgttggtg 6960 ttgggaggcc actttttaag tagcacaagg cacctagctc
gcagcaaggt gtccgaacca 7020 aagaagcggc tgcagtggtg caaacggggc
ggaaacggcg ggaaaaagcc acgggggcac 7080 gaattgaggc acgccctcga
atttgagacg agtcacggcc ccattcgccc gcgcaatggc 7140 tcgccaacgc
ccggtctttt gcaccacatc aggttacccc aagccaaacc tttgtgttaa 7200
aaagcttaac atattatacc gaacgtaggt ttgggcgggc ttgctccgtc tgtccaaggc
7260 aacatttata taagggtctg catcgccggc tcaattgaat cttttttctt
cttctcttct 7320 ctatattcat tcttgaatta aacacacatc aaccatggat
ccactagttc tagagcggcc 7380 gccaccgcgg cccgagattc cggcctcttc
ggccgccaag cgacccgggt ggacgtctag 7440 aggtacctag caattaacag
atagtttgcc ggtgataatt ctcttaacct cccacactcc 7500 tttgacataa
cgatttatgt aacgaaactg aaatttgacc agatattgtg tccgcggtgg 7560
agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
7620 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaac 7668
<210> SEQ ID NO 89 <211> LENGTH: 9048 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid pY130 <400> SEQUENCE:
89 gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
ctaactcaca 60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc
ggtttgcgta ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 300
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
360 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg 420 acaggactat aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
720 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc 780 tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct 960 acggggtctg
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1020
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1080 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc 1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact 1200 acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1380
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1440 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
aaggcgagtt 1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc
atggttatgg cagcactgca taattctctt 1620 actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740
gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
1800 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac 1860 tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt 1980 tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa 2040 tgtatttaga
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2160 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc 2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc
tccctttagg gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa
cttgattagg gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt
tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 2460
taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2520 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
aggctgcgca 2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt
acgccagctg gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa
cgccagggtt ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg
taatacgact cactataggg cgaattgggt accgggcccc 2760
ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct
2820 tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt
ctacactgat 2880 taattttcgg gccaataatt taaaaaaatc gtgttatata
atattatatg tattatatat 2940 atacatcatg atgatactga cagtcatgtc
ccattgctaa atagacagac tccatctgcc 3000 gcctccaact gatgttctca
atatttaagg ggtcatctcg cattgtttaa taataaacag 3060 actccatcta
ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt 3120
acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa
3180 tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat
aaatgcgtat 3240 gggaaatctt aaatatggat agcataaatg atatctgcat
tgcctaattc gaaatcaaca 3300 gcaacgaaaa aaatcccttg tacaacataa
atagtcatcg agaaatatca actatcaaag 3360 aacagctatt cacacgttac
tattgagatt attattggac gagaatcaca cactcaactg 3420 tctttctctc
ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct 3480
agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca
3540 aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc
aaaaagcttc 3600 tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc
attaaaggta tatatttatt 3660 tcttgttata taatcctttt gtttattaca
tgggctggat acataaaggt attttgattt 3720 aattttttgc ttaaattcaa
tcccccctcg ttcagtgtca actgtaatgg taggaaatta 3780 ccatactttt
gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga 3840
cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg
3900 ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta
caactatgta 3960 ctactgttga tgcatccaca acagtttgtt ttgttttttt
ttgttttttt tttttctaat 4020 gattcattac cgctatgtat acctacttgt
acttgtagta agccgggtta ttggcgttca 4080 attaatcata gacttatgaa
tctgcacggt gtgcgctgcg agttactttt agcttatgca 4140 tgctacttgg
gtgtaatatt gggatctgtt cggaaatcaa cggatgctca accgatttcg 4200
acagtaataa tttgaatcga atcggagcct aaaatgaacc cgagtatatc tcataaaatt
4260 ctcggtgaga ggtctgtgac tgtcagtaca aggtgccttc attatgccct
caaccttacc 4320 atacctcact gaatgtagtg tacctctaaa aatgaaatac
agtgccaaaa gccaaggcac 4380 tgagctcgtc taacggactt gatatacaac
caattaaaac aaatgaaaag aaatacagtt 4440 ctttgtatca tttgtaacaa
ttaccctgta caaactaagg tattgaaatc ccacaatatt 4500 cccaaagtcc
acccctttcc aaattgtcat gcctacaact catataccaa gcactaacct 4560
accaaacacc actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc
4620 accacactcg ttgggtgcag tcgccagctt aaagatatct atccacatca
gccacaactc 4680 ccttccttta ataaaccgac tacacccttg gctattgagg
ttatgagtga atatactgta 4740 gacaagacac tttcaagaag actgtttcca
aaacgtacca ctgtcctcca ctacaaacac 4800 acccaatctg cttcttctag
tcaaggttgc tacaccggta aattataaat catcatttca 4860 ttagcagggc
agggcccttt ttatagagtc ttatacacta gcggaccctg ccggtagacc 4920
aacccgcagg cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct
4980 tcttgagcag ctccttgacc ttgttggcaa caagtctccg acctcggagg
tggaggaaga 5040 gcctccgata tcggcggtag tgataccagc ctcgacggac
tccttgacgg cagcctcaac 5100 agcgtcaccg gcgggcttca tgttaagaga
gaacttgagc atcatggcgg cagacagaat 5160 ggtggcaatg gggttgacct
tctgcttgcc gagatcgggg gcagatccgt gacagggctc 5220 gtacagaccg
aacgcctcgt tggtgtcggg cagagaagcc agagaggcgg agggcagcag 5280
acccagagaa ccggggatga cggaggcctc gtcggagatg atatcgccaa acatgttggt
5340 ggtgatgatg ataccattca tcttggaggg ctgcttgatg aggatcatgg
cggccgagtc 5400 gatcagctgg tggttgagct cgagctgggg gaattcgtcc
ttgaggactc gagtgacagt 5460 ctttcgccaa agtcgagagg aggccagcac
gttggccttg tcaagagacc acacgggaag 5520 aggggggttg tgctgaaggg
ccaggaaggc ggccattcgg gcaattcgct caacctcagg 5580 aacggagtag
gtctcggtgt cggaagcgac gccagatccg tcatcctcct ttcgctctcc 5640
aaagtagata cctccgacga gctctcggac aatgatgaag tcggtgccct caacgtttcg
5700 gatgggggag agatcggcga gcttgggcga cagcagctgg cagggtcgca
ggttggcgta 5760 caggttcagg tcctttcgca gcttgaggag accctgctcg
ggtcgcacgt cggttcgtcc 5820 gtcgggagtg gtccatacgg tgttggcagc
gcctccgaca gcaccgagca taatagagtc 5880 agcctttcgg cagatgtcga
gagtagcgtc ggtgatgggc tcgccctcct tctcaatggc 5940 agctcctcca
atgagtcggt cctcaaacac aaactcggtg ccggaggcct cagcaacaga 6000
cttgagcacc ttgacggcct cggcaatcac ctcggggcca cagaagtcgc cgccgagaag
6060 aacaatcttc ttggagtcag tcttggtctt cttagtttcg ggttccattg
tggatgtgtg 6120 tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt
ggctggcaaa cgctcttgta 6180 tatatacgca cttttgcccg tgctatgtgg
aagactaaac ctccgaagat tgtgactcag 6240 gtagtgcggt atcggctagg
gacccaaacc ttgtcgatgc cgatagcgct atcgaacgta 6300 ccccagccgg
ccgggagtat gtcggagggg acatacgaga tcgtcaaggg tttgtggcca 6360
actggtattt aaatgatgtc gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg
6420 tggagaaagg ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgt
gcttggagat 6480 ggaagccggt agaaccgggc tgcttggggg gatttggggc
cgctgggctc caaagagggg 6540 taggcatttc gttggggtta cgtaattgcg
gcatttgggt cctgcgcgca tgtcccattg 6600 gtcagaatta gtccggatag
gagacttatc agccaatcac agcgccggat ccacctgtag 6660 gttgggttgg
gtgggagcac ccctccacag agtagagtca aacagcagca gcaacatgat 6720
agttgggggt gtgcgtgtta aaggaaaaaa aagaagcttg ggttatattc ccgctctatt
6780 tagaggttgc gggatagacg ccgacggagg gcaatggcgc tatggaacct
tgcggatatc 6840 catacgccgc ggcggactgc gtccgaacca gctccagcag
cgttttttcc gggccattga 6900 gccgactgcg accccgccaa cgtgtcttgg
cccacgcact catgtcatgt tggtgttggg 6960 aggccacttt ttaagtagca
caaggcacct agctcgcagc aaggtgtccg aaccaaagaa 7020 gcggctgcag
tggtgcaaac ggggcggaaa cggcgggaaa aagccacggg ggcacgaatt 7080
gaggcacgcc ctcgaatttg agacgagtca cggccccatt cgcccgcgca atggctcgcc
7140 aacgcccggt cttttgcacc acatcaggtt accccaagcc aaacctttgt
gttaaaaagc 7200 ttaacatatt ataccgaacg taggtttggg cgggcttgct
ccgtctgtcc aaggcaacat 7260 ttatataagg gtctgcatcg ccggctcaat
tgaatctttt ttcttcttct cttctctata 7320 ttcattcttg aattaaacac
acatcaacca tggcgactcg acagcgaact gccaccactg 7380 ttgtggtcga
ggaccttccc aaggtcactc ttgaggccaa gtctgaacct gtgttccccg 7440
atatcaagac catcaaggat gccattcccg cgcactgctt ccagccctcg ctcgtcacct
7500 cattctacta cgtcttccgc gattttgcta tggtctctgc cctcgtctgg
gctgctctca 7560 cctacatccc cagcatcccc gaccagaccc tccgcgtcgc
agcttggatg gtctacggct 7620 tcgtccaggg tctgttctgc accggtgtct
ggattctcgg ccatgagtgc ggccacggtg 7680 ctttctctct ccacggaaag
gtcaacaatg tgaccggctg gttcctccac tcgttcctcc 7740 tcgtccccta
cttcagctgg aagtactctc accaccgcca ccaccgcttc accggccaca 7800
tggatctcga catggctttc gtccccaaga ctgagcccaa gccctccaag tcgctcatga
7860 ttgctggcat tgacgtcgcc gagcttgttg aggacacccc cgctgctcag
atggtcaagc 7920 tcatcttcca ccagcttttc ggatggcagg cgtacctctt
cttcaacgct agctctggca 7980 agggcagcaa gcagtgggag cccaagactg
gcctctccaa gtggttccga gtcagtcact 8040 tcgagcctac cagcgctgtc
ttccgcccca acgaggccat cttcatcctc atctccgata 8100 tcggtcttgc
tctaatggga actgctctgt actttgcttc caagcaagtt ggtgtttcga 8160
ccattctctt cctctacctt gttccctacc tgtgggttca ccactggctc gttgccatta
8220 cctacctcca ccaccaccac accgagctcc ctcactacac cgctgagggc
tggacctacg 8280 tcaagggagc tctcgccact gtcgaccgtg agtttggctt
catcggaaag cacctcttcc 8340 acggtatcat tgagaagcac gttgttcacc
atctcttccc taagatcccc ttctacaagg 8400 ctgacgaggc caccgaggcc
atcaagcccg tcattggcga ccactactgc cacgacgacc 8460 gaagcttcct
gggccagctg tggaccatct tcggcacgct caagtacgtc gagcacgacc 8520
ctgcccgacc cggtgccatg cgatggaaca aggactaggc ggccgcatga gaagataaat
8580 atataaatac attgagatat taaatgcgct agattagaga gcctcatact
gctcggagag 8640 aagccaagac gagtactcaa aggggattac accatccata
tccacagaca caagctgggg 8700 aaaggttcta tatacacttt ccggaatacc
gtagtttccg atgttatcaa tgggggcagc 8760 caggatttca ggcacttcgg
tgtctcgggg tgaaatggcg ttcttggcct ccatcaagtc 8820 gtaccatgtc
ttcatttgcc tgtcaaagta aaacagaagc agatgaagaa tgaacttgaa 8880
gtgaaggaat ttaaatgtaa cgaaactgaa atttgaccag atattgtgtc cgcggtggag
8940 ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
catggtcata 9000 gctgtttcct gtgtgaaatt gttatccgct cacaagcttc
cacacaac 9048 <210> SEQ ID NO 90 <211> LENGTH: 8925
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Plasmid p138
<400> SEQUENCE: 90 gtacgagccg gaagcataaa gtgtaaagcc
tggggtgcct aatgagtgag ctaactcaca 60 ttaattgcgt tgcgctcact
gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 120 taatgaatcg
gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 180
tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
240 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
catgtgagca 300 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
tgctggcgtt tttccatagg 360 ctccgccccc ctgacgagca tcacaaaaat
cgacgctcaa gtcagaggtg gcgaaacccg 420 acaggactat aaagatacca
ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 480 ccgaccctgc
cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 540
tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
600 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
ctatcgtctt 660 gagtccaacc cggtaagaca cgacttatcg ccactggcag
cagccactgg taacaggatt 720 agcagagcga ggtatgtagg cggtgctaca
gagttcttga agtggtggcc taactacggc 780 tacactagaa ggacagtatt
tggtatctgc gctctgctga agccagttac cttcggaaaa 840 agagttggta
gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 900
tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
960
acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1020 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
atcaatctaa 1080 agtatatatg agtaaacttg gtctgacagt taccaatgct
taatcagtga ggcacctatc 1140 tcagcgatct gtctatttcg ttcatccata
gttgcctgac tccccgtcgt gtagataact 1200 acgatacggg agggcttacc
atctggcccc agtgctgcaa tgataccgcg agacccacgc 1260 tcaccggctc
cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 1320
ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
1380 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
catcgtggtg 1440 tcacgctcgt cgtttggtat ggcttcattc agctccggtt
cccaacgatc aaggcgagtt 1500 acatgatccc ccatgttgtg caaaaaagcg
gttagctcct tcggtcctcc gatcgttgtc 1560 agaagtaagt tggccgcagt
gttatcactc atggttatgg cagcactgca taattctctt 1620 actgtcatgc
catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 1680
tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
1740 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
ggggcgaaaa 1800 ctctcaagga tcttaccgct gttgagatcc agttcgatgt
aacccactcg tgcacccaac 1860 tgatcttcag catcttttac tttcaccagc
gtttctgggt gagcaaaaac aggaaggcaa 1920 aatgccgcaa aaaagggaat
aagggcgaca cggaaatgtt gaatactcat actcttcctt 1980 tttcaatatt
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 2040
tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
2100 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
cagcgtgacc 2160 gctacacttg ccagcgccct agcgcccgct cctttcgctt
tcttcccttc ctttctcgcc 2220 acgttcgccg gctttccccg tcaagctcta
aatcgggggc tccctttagg gttccgattt 2280 agtgctttac ggcacctcga
ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 2340 ccatcgccct
gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2400
ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
2460 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
acaaaaattt 2520 aacgcgaatt ttaacaaaat attaacgctt acaatttcca
ttcgccattc aggctgcgca 2580 actgttggga agggcgatcg gtgcgggcct
cttcgctatt acgccagctg gcgaaagggg 2640 gatgtgctgc aaggcgatta
agttgggtaa cgccagggtt ttcccagtca cgacgttgta 2700 aaacgacggc
cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc 2760
ccctcgaggt cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct
2820 tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt
ctacactgat 2880 taattttcgg gccaataatt taaaaaaatc gtgttatata
atattatatg tattatatat 2940 atacatcatg atgatactga cagtcatgtc
ccattgctaa atagacagac tccatctgcc 3000 gcctccaact gatgttctca
atatttaagg ggtcatctcg cattgtttaa taataaacag 3060 actccatcta
ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt 3120
acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa
3180 tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat
aaatgcgtat 3240 gggaaatctt aaatatggat agcataaatg atatctgcat
tgcctaattc gaaatcaaca 3300 gcaacgaaaa aaatcccttg tacaacataa
atagtcatcg agaaatatca actatcaaag 3360 aacagctatt cacacgttac
tattgagatt attattggac gagaatcaca cactcaactg 3420 tctttctctc
ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct 3480
agtcatttca tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca
3540 aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc
aaaaagcttc 3600 tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc
attaaaggta tatatttatt 3660 tcttgttata taatcctttt gtttattaca
tgggctggat acataaaggt attttgattt 3720 aattttttgc ttaaattcaa
tcccccctcg ttcagtgtca actgtaatgg taggaaatta 3780 ccatactttt
gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga 3840
cgttccgcag aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg
3900 ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta
caactatgta 3960 ctactgttga tgcatccaca acagtttgtt ttgttttttt
ttgttttttt tttttctaat 4020 gattcattac cgctatgtat acctacttgt
acttgtagta agccgggtta ttggcgttca 4080 attaatcata gacttatgaa
tctgcacggt gtgcgctgcg agttactttt agcttatgca 4140 tgctacttgg
gtgtaatatt gggatctgtt cggaaatcaa cggatgctca accgatttcg 4200
acagtaataa tttgaatcga atcggagcct aaaatgaacc cgagtatatc tcataaaatt
4260 ctcggtgaga ggtctgtgac tgtcagtaca aggtgccttc attatgccct
caaccttacc 4320 atacctcact gaatgtagtg tacctctaaa aatgaaatac
agtgccaaaa gccaaggcac 4380 tgagctcgtc taacggactt gatatacaac
caattaaaac aaatgaaaag aaatacagtt 4440 ctttgtatca tttgtaacaa
ttaccctgta caaactaagg tattgaaatc ccacaatatt 4500 cccaaagtcc
acccctttcc aaattgtcat gcctacaact catataccaa gcactaacct 4560
accaaacacc actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc
4620 accacactcg ttgggtgcag tcgccagctt aaagatatct atccacatca
gccacaactc 4680 ccttccttta ataaaccgac tacacccttg gctattgagg
ttatgagtga atatactgta 4740 gacaagacac tttcaagaag actgtttcca
aaacgtacca ctgtcctcca ctacaaacac 4800 acccaatctg cttcttctag
tcaaggttgc tacaccggta aattataaat catcatttca 4860 ttagcagggc
agggcccttt ttatagagtc ttatacacta gcggaccctg ccggtagacc 4920
aacccgcagg cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct
4980 tcttgagcag ctccttgacc ttgttggcaa caagtctccg acctcggagg
tggaggaaga 5040 gcctccgata tcggcggtag tgataccagc ctcgacggac
tccttgacgg cagcctcaac 5100 agcgtcaccg gcgggcttca tgttaagaga
gaacttgagc atcatggcgg cagacagaat 5160 ggtggcaatg gggttgacct
tctgcttgcc gagatcgggg gcagatccgt gacagggctc 5220 gtacagaccg
aacgcctcgt tggtgtcggg cagagaagcc agagaggcgg agggcagcag 5280
acccagagaa ccggggatga cggaggcctc gtcggagatg atatcgccaa acatgttggt
5340 ggtgatgatg ataccattca tcttggaggg ctgcttgatg aggatcatgg
cggccgagtc 5400 gatcagctgg tggttgagct cgagctgggg gaattcgtcc
ttgaggactc gagtgacagt 5460 ctttcgccaa agtcgagagg aggccagcac
gttggccttg tcaagagacc acacgggaag 5520 aggggggttg tgctgaaggg
ccaggaaggc ggccattcgg gcaattcgct caacctcagg 5580 aacggagtag
gtctcggtgt cggaagcgac gccagatccg tcatcctcct ttcgctctcc 5640
aaagtagata cctccgacga gctctcggac aatgatgaag tcggtgccct caacgtttcg
5700 gatgggggag agatcggcga gcttgggcga cagcagctgg cagggtcgca
ggttggcgta 5760 caggttcagg tcctttcgca gcttgaggag accctgctcg
ggtcgcacgt cggttcgtcc 5820 gtcgggagtg gtccatacgg tgttggcagc
gcctccgaca gcaccgagca taatagagtc 5880 agcctttcgg cagatgtcga
gagtagcgtc ggtgatgggc tcgccctcct tctcaatggc 5940 agctcctcca
atgagtcggt cctcaaacac aaactcggtg ccggaggcct cagcaacaga 6000
cttgagcacc ttgacggcct cggcaatcac ctcggggcca cagaagtcgc cgccgagaag
6060 aacaatcttc ttggagtcag tcttggtctt cttagtttcg ggttccattg
tggatgtgtg 6120 tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt
ggctggcaaa cgctcttgta 6180 tatatacgca cttttgcccg tgctatgtgg
aagactaaac ctccgaagat tgtgactcag 6240 gtagtgcggt atcggctagg
gacccaaacc ttgtcgatgc cgatagcgct atcgaacgta 6300 ccccagccgg
ccgggagtat gtcggagggg acatacgaga tcgtcaaggg tttgtggcca 6360
actggtattt aaatgatgtc gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg
6420 tggagaaagg ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgt
gcttggagat 6480 ggaagccggt agaaccgggc tgcttggggg gatttggggc
cgctgggctc caaagagggg 6540 taggcatttc gttggggtta cgtaattgcg
gcatttgggt cctgcgcgca tgtcccattg 6600 gtcagaatta gtccggatag
gagacttatc agccaatcac agcgccggat ccacctgtag 6660 gttgggttgg
gtgggagcac ccctccacag agtagagtca aacagcagca gcaacatgat 6720
agttgggggt gtgcgtgtta aaggaaaaaa aagaagcttg ggttatattc ccgctctatt
6780 tagaggttgc gggatagacg ccgacggagg gcaatggcgc tatggaacct
tgcggatatc 6840 catacgccgc ggcggactgc gtccgaacca gctccagcag
cgttttttcc gggccattga 6900 gccgactgcg accccgccaa cgtgtcttgg
cccacgcact catgtcatgt tggtgttggg 6960 aggccacttt ttaagtagca
caaggcacct agctcgcagc aaggtgtccg aaccaaagaa 7020 gcggctgcag
tggtgcaaac ggggcggaaa cggcgggaaa aagccacggg ggcacgaatt 7080
gaggcacgcc ctcgaatttg agacgagtca cggccccatt cgcccgcgca atggctcgcc
7140 aacgcccggt cttttgcacc acatcaggtt accccaagcc aaacctttgt
gttaaaaagc 7200 ttaacatatt ataccgaacg taggtttggg cgggcttgct
ccgtctgtcc aaggcaacat 7260 ttatataagg gtctgcatcg ccggctcaat
tgaatctttt ttcttcttct cttctctata 7320 ttcattcttg aattaaacac
acatcaacca tggctaccaa gcagccctac cagttcccta 7380 ctctgaccga
gatcaagcga tctcttccct ccgagtgctt tgaagcctcg gtccctctgt 7440
ccttgtacta caccgtgcga atcgtcgcta ttgccgttgc tctggccttc ggactcaact
7500 acgctcgagc ccttcccgtg gtcgagtctc tgtgggcact cgacgctgcc
ctttgttgcg 7560 gttacgttct gctccaaggc attgtcttct ggggattctt
taccgtgggt cacgatgctg 7620 gacatggtgc cttctctcga taccacctgc
tcaactttgt cgttggcacc tttatccact 7680 ccctcattct tactcccttc
gagtcgtgga agctcacaca tcgacaccat cacaagaaca 7740 ccggaaacat
cgaccgagac gaaatcttct accctcagcg aaaggccgac gatcatcctc 7800
tgtctcgaaa cctcgtcctg gctctcggtg ccgcttggtt tgcctacctt gtcgagggct
7860 ttcctccccg aaaggtcaac cacttcaacc ccttcgaacc tctgtttgtg
cgacaggtgg 7920 ctgccgttgt catttccctc tctgctcact tcgccgtcct
ggcactgtcc gtgtatctga 7980 gctttcagtt cggtctcaag acaatggctc
tgtactacta tggacccgtc ttcgtgttcg 8040 gctccatgct cgtcattact
acctttctgc atcacaatga cgaggaaact ccttggtacg 8100 gagattccga
ctggacctac gtcaagggca acttgtcttc cgtggaccga tcttacggtg 8160
ccttcatcga caacctctcg cacaacattg gcacacacca gatccaccat ctgtttccca
8220 tcattcctca ctacaagctc aaccgagcca ccgctgcctt ccaccaggcc
tttcccgaac 8280 ttgtccgaaa gagcgacgag cccattctca aggctttctg
gagagttggt cgactttacg 8340 ccaactacgg agtcgtggat cccgacgcaa
agctgtttac tctcaaggag gccaaagctg 8400 cctccgaggc tgccaccaag
accaaggcta cttaagcggc cgcatgagaa gataaatata 8460
taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct cggagagaag
8520 ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa
gctggggaaa 8580 ggttctatat acactttccg gaataccgta gtttccgatg
ttatcaatgg gggcagccag 8640 gatttcaggc acttcggtgt ctcggggtga
aatggcgttc ttggcctcca tcaagtcgta 8700 ccatgtcttc atttgcctgt
caaagtaaaa cagaagcaga tgaagaatga acttgaagtg 8760 aaggaattta
aatgtaacga aactgaaatt tgaccagata ttgtgtccgc ggtggagctc 8820
cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
8880 gtttcctgtg tgaaattgtt atccgctcac aagcttccac acaac 8925
<210> SEQ ID NO 91 <211> LENGTH: 8925 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Plasmid p139 <400> SEQUENCE:
91 gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
ctaactcaca 60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc
ggtttgcgta ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 300
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
360 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg 420 acaggactat aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
720 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc 780 tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct 960 acggggtctg
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1020
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1080 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc 1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact 1200 acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1380
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1440 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
aaggcgagtt 1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc
atggttatgg cagcactgca taattctctt 1620 actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740
gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
1800 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac 1860 tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt 1980 tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa 2040 tgtatttaga
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2160 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
ctttctcgcc 2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc
tccctttagg gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa
cttgattagg gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt
tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 2460
taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2520 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
aggctgcgca 2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt
acgccagctg gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa
cgccagggtt ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg
taatacgact cactataggg cgaattgggt accgggcccc 2760 ccctcgaggt
cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct 2820
tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat
2880 taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg
tattatatat 2940 atacatcatg atgatactga cagtcatgtc ccattgctaa
atagacagac tccatctgcc 3000 gcctccaact gatgttctca atatttaagg
ggtcatctcg cattgtttaa taataaacag 3060 actccatcta ccgcctccaa
atgatgttct caaaatatat tgtatgaact tatttttatt 3120 acttagtatt
attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa 3180
tttataatgg cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat
3240 gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc
gaaatcaaca 3300 gcaacgaaaa aaatcccttg tacaacataa atagtcatcg
agaaatatca actatcaaag 3360 aacagctatt cacacgttac tattgagatt
attattggac gagaatcaca cactcaactg 3420 tctttctctc ttctagaaat
acaggtacaa gtatgtacta ttctcattgt tcatacttct 3480 agtcatttca
tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca 3540
aattcaacaa ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc
3600 tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta
tatatttatt 3660 tcttgttata taatcctttt gtttattaca tgggctggat
acataaaggt attttgattt 3720 aattttttgc ttaaattcaa tcccccctcg
ttcagtgtca actgtaatgg taggaaatta 3780 ccatactttt gaagaagcaa
aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga 3840 cgttccgcag
aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg 3900
ctccctgaga tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta
3960 ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt
tttttctaat 4020 gattcattac cgctatgtat acctacttgt acttgtagta
agccgggtta ttggcgttca 4080 attaatcata gacttatgaa tctgcacggt
gtgcgctgcg agttactttt agcttatgca 4140 tgctacttgg gtgtaatatt
gggatctgtt cggaaatcaa cggatgctca accgatttcg 4200 acagtaataa
tttgaatcga atcggagcct aaaatgaacc cgagtatatc tcataaaatt 4260
ctcggtgaga ggtctgtgac tgtcagtaca aggtgccttc attatgccct caaccttacc
4320 atacctcact gaatgtagtg tacctctaaa aatgaaatac agtgccaaaa
gccaaggcac 4380 tgagctcgtc taacggactt gatatacaac caattaaaac
aaatgaaaag aaatacagtt 4440 ctttgtatca tttgtaacaa ttaccctgta
caaactaagg tattgaaatc ccacaatatt 4500 cccaaagtcc acccctttcc
aaattgtcat gcctacaact catataccaa gcactaacct 4560 accaaacacc
actaaaaccc cacaaaatat atcttaccga atatacagta acaagctacc 4620
accacactcg ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc
4680 ccttccttta ataaaccgac tacacccttg gctattgagg ttatgagtga
atatactgta 4740 gacaagacac tttcaagaag actgtttcca aaacgtacca
ctgtcctcca ctacaaacac 4800 acccaatctg cttcttctag tcaaggttgc
tacaccggta aattataaat catcatttca 4860 ttagcagggc agggcccttt
ttatagagtc ttatacacta gcggaccctg ccggtagacc 4920 aacccgcagg
cgcgtcagtt tgctccttcc atcaatgcgt cgtagaaacg acttactcct 4980
tcttgagcag ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga
5040 gcctccgata tcggcggtag tgataccagc ctcgacggac tccttgacgg
cagcctcaac 5100 agcgtcaccg gcgggcttca tgttaagaga gaacttgagc
atcatggcgg cagacagaat 5160 ggtggcaatg gggttgacct tctgcttgcc
gagatcgggg gcagatccgt gacagggctc 5220 gtacagaccg aacgcctcgt
tggtgtcggg cagagaagcc agagaggcgg agggcagcag 5280 acccagagaa
ccggggatga cggaggcctc gtcggagatg atatcgccaa acatgttggt 5340
ggtgatgatg ataccattca tcttggaggg ctgcttgatg aggatcatgg cggccgagtc
5400 gatcagctgg tggttgagct cgagctgggg gaattcgtcc ttgaggactc
gagtgacagt 5460 ctttcgccaa agtcgagagg aggccagcac gttggccttg
tcaagagacc acacgggaag 5520 aggggggttg tgctgaaggg ccaggaaggc
ggccattcgg gcaattcgct caacctcagg 5580 aacggagtag gtctcggtgt
cggaagcgac gccagatccg tcatcctcct ttcgctctcc 5640 aaagtagata
cctccgacga gctctcggac aatgatgaag tcggtgccct caacgtttcg 5700
gatgggggag agatcggcga gcttgggcga cagcagctgg cagggtcgca ggttggcgta
5760 caggttcagg tcctttcgca gcttgaggag accctgctcg ggtcgcacgt
cggttcgtcc 5820 gtcgggagtg gtccatacgg tgttggcagc gcctccgaca
gcaccgagca taatagagtc 5880 agcctttcgg cagatgtcga gagtagcgtc
ggtgatgggc tcgccctcct tctcaatggc 5940 agctcctcca atgagtcggt
cctcaaacac aaactcggtg ccggaggcct cagcaacaga 6000 cttgagcacc
ttgacggcct cggcaatcac ctcggggcca cagaagtcgc cgccgagaag 6060
aacaatcttc ttggagtcag tcttggtctt cttagtttcg ggttccattg tggatgtgtg
6120 tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt ggctggcaaa
cgctcttgta 6180 tatatacgca cttttgcccg tgctatgtgg aagactaaac
ctccgaagat tgtgactcag 6240 gtagtgcggt atcggctagg gacccaaacc
ttgtcgatgc cgatagcgct atcgaacgta 6300 ccccagccgg ccgggagtat
gtcggagggg acatacgaga tcgtcaaggg tttgtggcca 6360 actggtattt
aaatgatgtc gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg 6420
tggagaaagg ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgt gcttggagat
6480 ggaagccggt agaaccgggc tgcttggggg gatttggggc cgctgggctc
caaagagggg 6540 taggcatttc gttggggtta cgtaattgcg gcatttgggt
cctgcgcgca tgtcccattg 6600 gtcagaatta gtccggatag gagacttatc
agccaatcac agcgccggat ccacctgtag 6660 gttgggttgg gtgggagcac
ccctccacag agtagagtca aacagcagca gcaacatgat 6720 agttgggggt
gtgcgtgtta aaggaaaaaa aagaagcttg ggttatattc ccgctctatt 6780
tagaggttgc gggatagacg ccgacggagg gcaatggcgc tatggaacct tgcggatatc
6840 catacgccgc ggcggactgc gtccgaacca gctccagcag cgttttttcc
gggccattga 6900 gccgactgcg accccgccaa cgtgtcttgg cccacgcact
catgtcatgt tggtgttggg 6960 aggccacttt ttaagtagca caaggcacct
agctcgcagc aaggtgtccg aaccaaagaa 7020 gcggctgcag tggtgcaaac
ggggcggaaa cggcgggaaa aagccacggg ggcacgaatt 7080 gaggcacgcc
ctcgaatttg agacgagtca cggccccatt cgcccgcgca atggctcgcc 7140
aacgcccggt cttttgcacc acatcaggtt accccaagcc aaacctttgt gttaaaaagc
7200 ttaacatatt ataccgaacg taggtttggg cgggcttgct ccgtctgtcc
aaggcaacat 7260 ttatataagg gtctgcatcg ccggctcaat tgaatctttt
ttcttcttct cttctctata 7320 ttcattcttg aattaaacac acatcaacca
tggctaccaa gcagccctac cagttcccta 7380 ctctgaccga gatcaagcga
tctctgccct ccgagtgttt cgaggcctcc gtgcctctct 7440 ctctgtacta
caccgttcga tgcctggtca ttgctgtgtc gctcgccttc ggacttcacc 7500
atgcacgatc tctgcccgtt gtcgaaggcc tctgggctct ggatgccgct ctctgcaccg
7560 gttacgtgct gctccagggc atcgtcttct ggggattctt tactgttggt
cacgacgctg 7620 gacatggtgc cttctcccga taccacctgc tcaactttgt
catcggaacc ttcattcact 7680 ctctcatcct tacacccttc gagtcctgga
agctcaccca cagacaccat cacaagaaca 7740 ctggcaacat cgaccgagac
gaaatcttct accctcaacg aaaggccgac gatcatcctc 7800 tgtctcgaaa
cctcattctg gctttgggtg cagcctggtt tgcctacctg gtcgaaggct 7860
ttcctccccg aaaggtcaac cacttcaacc ccttcgagcc tctctttgtt cgacaggtct
7920 ctgccgtggt catttcgctg gctgcgcact ttggagtggc tgccctgtcc
atctacctca 7980 gcctgcagtt cggcttcaag actatggcca tctactacta
tggtcccgtc tttgtgttcg 8040 gatccatgct cgtcattact acctttcttc
atcacaacga cgaagagaca ccttggtacg 8100 cagattcgga gtggacctac
gtcaaaggca acctgtcctc tgtcgaccga tcctacggtg 8160 ccctcatcga
caacctttct cacaacatcg gaacccacca gattcatcac ctctttccca 8220
tcattcctca ctacaagctc aagcgagcta ccgaggcctt ccatcaagcc tttcccgagc
8280 tggttcgaaa gtccgacgaa cccatcatca aggccttttt cagagtcggc
cgactctacg 8340 caaactacgg tgtggtcgac tcggatgcca agctgttcac
tctcaaggag gccaaggctg 8400 tttccgaagc cgctaccaag actaaggcca
cctaagcggc cgcatgagaa gataaatata 8460 taaatacatt gagatattaa
atgcgctaga ttagagagcc tcatactgct cggagagaag 8520 ccaagacgag
tactcaaagg ggattacacc atccatatcc acagacacaa gctggggaaa 8580
ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg gggcagccag
8640 gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca
tcaagtcgta 8700 ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga
tgaagaatga acttgaagtg 8760 aaggaattta aatgtaacga aactgaaatt
tgaccagata ttgtgtccgc ggtggagctc 8820 cagcttttgt tccctttagt
gagggttaat ttcgagcttg gcgtaatcat ggtcatagct 8880 gtttcctgtg
tgaaattgtt atccgctcac aagcttccac acaac 8925 <210> SEQ ID NO
92 <211> LENGTH: 8919 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pY140 <400> SEQUENCE: 92
gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
60 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
ccagctgcat 120 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc 180 tcgctcactg actcgctgcg ctcggtcgtt
cggctgcggc gagcggtatc agctcactca 240 aaggcggtaa tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca 300 aaaggccagc
aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 360
ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
420 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt 480 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt 540 tctcatagct cacgctgtag gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc 600 tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660 gagtccaacc
cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 720
agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
780 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
cttcggaaaa 840 agagttggta gctcttgatc cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt 900 tgcaagcagc agattacgcg cagaaaaaaa
ggatctcaag aagatccttt gatcttttct 960 acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta 1020 tcaaaaagga
tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 1080
agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
1140 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
gtagataact 1200 acgatacggg agggcttacc atctggcccc agtgctgcaa
tgataccgcg agacccacgc 1260 tcaccggctc cagatttatc agcaataaac
cagccagccg gaagggccga gcgcagaagt 1320 ggtcctgcaa ctttatccgc
ctccatccag tctattaatt gttgccggga agctagagta 1380 agtagttcgc
cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1440
tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
1500 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc 1560 agaagtaagt tggccgcagt gttatcactc atggttatgg
cagcactgca taattctctt 1620 actgtcatgc catccgtaag atgcttttct
gtgactggtg agtactcaac caagtcattc 1680 tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1740 gcgccacata
gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1800
ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
1860 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
aggaaggcaa 1920 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
gaatactcat actcttcctt 1980 tttcaatatt attgaagcat ttatcagggt
tattgtctca tgagcggata catatttgaa 2040 tgtatttaga aaaataaaca
aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2100 gacgcgccct
gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2160
gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
2220 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
gttccgattt 2280 agtgctttac ggcacctcga ccccaaaaaa cttgattagg
gtgatggttc acgtagtggg 2340 ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt ctttaatagt 2400 ggactcttgt tccaaactgg
aacaacactc aaccctatct cggtctattc ttttgattta 2460 taagggattt
tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 2520
aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
2580 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
gcgaaagggg 2640 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
ttcccagtca cgacgttgta 2700 aaacgacggc cagtgaattg taatacgact
cactataggg cgaattgggt accgggcccc 2760 ccctcgaggt cgatggtgtc
gataagcttg atatcgaatt catgtcacac aaaccgatct 2820 tcgcctcaag
gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat 2880
taattttcgg gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat
2940 atacatcatg atgatactga cagtcatgtc ccattgctaa atagacagac
tccatctgcc 3000 gcctccaact gatgttctca atatttaagg ggtcatctcg
cattgtttaa taataaacag 3060 actccatcta ccgcctccaa atgatgttct
caaaatatat tgtatgaact tatttttatt 3120 acttagtatt attagacaac
ttacttgctt tatgaaaaac acttcctatt taggaaacaa 3180 tttataatgg
cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat 3240
gggaaatctt aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca
3300 gcaacgaaaa aaatcccttg tacaacataa atagtcatcg agaaatatca
actatcaaag 3360 aacagctatt cacacgttac tattgagatt attattggac
gagaatcaca cactcaactg 3420 tctttctctc ttctagaaat acaggtacaa
gtatgtacta ttctcattgt tcatacttct 3480 agtcatttca tcccacatat
tccttggatt tctctccaat gaatgacatt ctatcttgca 3540 aattcaacaa
ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc 3600
tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt
3660 tcttgttata taatcctttt gtttattaca tgggctggat acataaaggt
attttgattt 3720 aattttttgc ttaaattcaa tcccccctcg ttcagtgtca
actgtaatgg taggaaatta 3780 ccatactttt gaagaagcaa aaaaaatgaa
agaaaaaaaa aatcgtattt ccaggttaga 3840 cgttccgcag aatctagaat
gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg 3900 ctccctgaga
tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta 3960
ctactgttga tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat
4020 gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta
ttggcgttca 4080 attaatcata gacttatgaa tctgcacggt gtgcgctgcg
agttactttt agcttatgca 4140 tgctacttgg gtgtaatatt gggatctgtt
cggaaatcaa cggatgctca accgatttcg 4200 acagtaataa tttgaatcga
atcggagcct aaaatgaacc cgagtatatc tcataaaatt 4260 ctcggtgaga
ggtctgtgac tgtcagtaca aggtgccttc attatgccct caaccttacc 4320
atacctcact gaatgtagtg tacctctaaa aatgaaatac agtgccaaaa gccaaggcac
4380 tgagctcgtc taacggactt gatatacaac caattaaaac aaatgaaaag
aaatacagtt 4440 ctttgtatca tttgtaacaa ttaccctgta caaactaagg
tattgaaatc ccacaatatt 4500 cccaaagtcc acccctttcc aaattgtcat
gcctacaact catataccaa gcactaacct 4560 accaaacacc actaaaaccc
cacaaaatat atcttaccga atatacagta acaagctacc 4620 accacactcg
ttgggtgcag tcgccagctt aaagatatct atccacatca gccacaactc 4680
ccttccttta ataaaccgac tacacccttg gctattgagg ttatgagtga atatactgta
4740 gacaagacac tttcaagaag actgtttcca aaacgtacca ctgtcctcca
ctacaaacac 4800 acccaatctg cttcttctag tcaaggttgc tacaccggta
aattataaat catcatttca 4860 ttagcagggc agggcccttt ttatagagtc
ttatacacta gcggaccctg ccggtagacc 4920 aacccgcagg cgcgtcagtt
tgctccttcc atcaatgcgt cgtagaaacg acttactcct 4980 tcttgagcag
ctccttgacc ttgttggcaa caagtctccg acctcggagg tggaggaaga 5040
gcctccgata tcggcggtag tgataccagc ctcgacggac tccttgacgg cagcctcaac
5100 agcgtcaccg gcgggcttca tgttaagaga gaacttgagc atcatggcgg
cagacagaat 5160 ggtggcaatg gggttgacct tctgcttgcc gagatcgggg
gcagatccgt gacagggctc 5220 gtacagaccg aacgcctcgt tggtgtcggg
cagagaagcc agagaggcgg agggcagcag 5280 acccagagaa ccggggatga
cggaggcctc gtcggagatg atatcgccaa acatgttggt 5340 ggtgatgatg
ataccattca tcttggaggg ctgcttgatg aggatcatgg cggccgagtc 5400
gatcagctgg tggttgagct cgagctgggg gaattcgtcc ttgaggactc gagtgacagt
5460 ctttcgccaa agtcgagagg aggccagcac gttggccttg tcaagagacc
acacgggaag 5520 aggggggttg tgctgaaggg ccaggaaggc ggccattcgg
gcaattcgct caacctcagg 5580 aacggagtag gtctcggtgt cggaagcgac
gccagatccg tcatcctcct ttcgctctcc 5640 aaagtagata cctccgacga
gctctcggac aatgatgaag tcggtgccct caacgtttcg 5700 gatgggggag
agatcggcga gcttgggcga cagcagctgg cagggtcgca ggttggcgta 5760
caggttcagg tcctttcgca gcttgaggag accctgctcg ggtcgcacgt cggttcgtcc
5820 gtcgggagtg gtccatacgg tgttggcagc gcctccgaca gcaccgagca
taatagagtc 5880 agcctttcgg cagatgtcga gagtagcgtc ggtgatgggc
tcgccctcct tctcaatggc 5940 agctcctcca atgagtcggt cctcaaacac
aaactcggtg ccggaggcct cagcaacaga 6000 cttgagcacc ttgacggcct
cggcaatcac ctcggggcca cagaagtcgc cgccgagaag 6060 aacaatcttc
ttggagtcag tcttggtctt cttagtttcg ggttccattg tggatgtgtg 6120
tggttgtatg tgtgatgtgg tgtgtggagt gaaaatctgt ggctggcaaa cgctcttgta
6180 tatatacgca cttttgcccg tgctatgtgg aagactaaac ctccgaagat
tgtgactcag 6240 gtagtgcggt atcggctagg gacccaaacc ttgtcgatgc
cgatagcgct atcgaacgta 6300 ccccagccgg ccgggagtat gtcggagggg
acatacgaga tcgtcaaggg tttgtggcca 6360 actggtattt aaatgatgtc
gacgcagtag gatgtcctgc acgggtcttt ttgtggggtg 6420 tggagaaagg
ggtgcttgga gatggaagcc ggtagaaccg ggctgcttgt gcttggagat 6480
ggaagccggt agaaccgggc tgcttggggg gatttggggc cgctgggctc caaagagggg
6540 taggcatttc gttggggtta cgtaattgcg gcatttgggt cctgcgcgca
tgtcccattg 6600 gtcagaatta gtccggatag gagacttatc agccaatcac
agcgccggat ccacctgtag 6660 gttgggttgg gtgggagcac ccctccacag
agtagagtca aacagcagca gcaacatgat 6720 agttgggggt gtgcgtgtta
aaggaaaaaa aagaagcttg ggttatattc ccgctctatt 6780 tagaggttgc
gggatagacg ccgacggagg gcaatggcgc tatggaacct tgcggatatc 6840
catacgccgc ggcggactgc gtccgaacca gctccagcag cgttttttcc gggccattga
6900 gccgactgcg accccgccaa cgtgtcttgg cccacgcact catgtcatgt
tggtgttggg 6960 aggccacttt ttaagtagca caaggcacct agctcgcagc
aaggtgtccg aaccaaagaa 7020 gcggctgcag tggtgcaaac ggggcggaaa
cggcgggaaa aagccacggg ggcacgaatt 7080 gaggcacgcc ctcgaatttg
agacgagtca cggccccatt cgcccgcgca atggctcgcc 7140 aacgcccggt
cttttgcacc acatcaggtt accccaagcc aaacctttgt gttaaaaagc 7200
ttaacatatt ataccgaacg taggtttggg cgggcttgct ccgtctgtcc aaggcaacat
7260 ttatataagg gtctgcatcg ccggctcaat tgaatctttt ttcttcttct
cttctctata 7320 ttcattcttg aattaaacac acatcaacca tggcttcctc
taccgttgcc gctccctacg 7380 agttccctac tctcaccgag atcaagcgat
ccctgcctgc ccactgcttc gaagcctctg 7440 ttccctggtc cctctactat
accgtgcgag ctctgggcat tgccggttcc cttgctctcg 7500 gactgtacta
tgctcgagcc cttgctatcg tgcaggagtt tgcactgctc gatgccgtcc 7560
tttgcactgg ctacattctg ctccagggta tcgtgttctg gggattcttt accatcggtc
7620 acgactgtgg acatggtgcc ttctcgcgat cccacctgct caacttctct
gttggcacac 7680 tcattcactc catcattctg actccctacg agtcgtggaa
gatcagccat cgacaccatc 7740 acaagaacac cggcaacatc gacaaggatg
agatcttcta ccctcagcga gaagccgact 7800 ctcatcccct gtcccgacac
atggtcatct cccttggttc ggcttggttt gcctacctcg 7860 ttgctggatt
tcctccccga aaggtcaacc acttcaatcc ctgggagcct ctctacctgc 7920
gaagaatgtc tgccgtcatc atttccctcg gctctctcgt ggcctttgct ggtctgtacg
7980 cctaccttac ctacgtctac ggcctcaaga ccatggctct gtattacttc
gcacctctct 8040 ttggattcgc caccatgctg gttgtcacta ccttcctcca
tcacaacgac gaggaaactc 8100 cctggtacgc cgattcggag tggacctatg
tcaagggcaa cttgtcctct gtggaccgaa 8160 gctacggagc cctcatcgac
aacctgtccc acaacattgg tacacatcag atccaccatc 8220 tgtttcccat
cattcctcac tacaagctca acgaggccac tgctgccttc gctcaggcct 8280
ttcccgaact ggtgcgaaag tcggcttctc ccatcattcc caccttcatc cgaattggtc
8340 ttatgtacgc caagtacggc gtggtcgaca aggatgccaa gatgtttacc
ctcaaggagg 8400 ccaaggctgc caagaccaaa gccaactaag cggccgcatg
agaagataaa tatataaata 8460 cattgagata ttaaatgcgc tagattagag
agcctcatac tgctcggaga gaagccaaga 8520 cgagtactca aaggggatta
caccatccat atccacagac acaagctggg gaaaggttct 8580 atatacactt
tccggaatac cgtagtttcc gatgttatca atgggggcag ccaggatttc 8640
aggcacttcg gtgtctcggg gtgaaatggc gttcttggcc tccatcaagt cgtaccatgt
8700 cttcatttgc ctgtcaaagt aaaacagaag cagatgaaga atgaacttga
agtgaaggaa 8760 tttaaatgta acgaaactga aatttgacca gatattgtgt
ccgcggtgga gctccagctt 8820 ttgttccctt tagtgagggt taatttcgag
cttggcgtaa tcatggtcat agctgtttcc 8880 tgtgtgaaat tgttatccgc
tcacaagctt ccacacaac 8919 <210> SEQ ID NO 93 <211>
LENGTH: 6267 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pY137 <400> SEQUENCE: 93 taactttggc cggcctttac
ctgcaggata acttcgtata atgtatgcta tacgaagtta 60 tgaattctgt
aatattggga tctgttcgga aatcaacgga tgctcaaccg atttcgacag 120
taataatttg aatcgaatcg gagcctaaaa tgaacccgag tatatctcat aaaattctcg
180 gtgagaggtc tgtgactgtc agtacaaggt gccttcatta tgccctcaac
cttaccatac 240 ctcactgaat gtagtgtacc tctaaaaatg aaatacagtg
ccaaaagcca aggcactgag 300 ctcgtctaac ggacttgata tacaaccaat
taaaacaaat gaaaagaaat acagttcttt 360 gtatcatttg taacaattac
cctgtacaaa ctaaggtatt gaaatcccac aatattccca 420 aagtccaccc
ctttccaaat tgtcatgcct acaactcata taccaagcac taacctacca 480
aacaccacta aaaccccaca aaatatatct taccgaatat acagtaacaa gctaccacca
540 cactcgttgg gtgcagtcgc cagcttaaag atatctatcc acatcagcca
caactccctt 600 cctttaataa accgactaca cccttggcta ttgaggttat
gagtgaatat actgtagaca 660 agacactttc aagaagactg tttccaaaac
gtaccactgt cctccactac aaacacaccc 720 aatctgcttc ttctagtcaa
ggttgctaca ccggtaaatt ataaatcatc atttcattag 780 cagggcaggg
ccctttttat agagtcttat acactagcgg accctgccgg tagaccaacc 840
cgcaggcgcg tcagtttgct ccttccatca atgcgtcgta gaaacgactt actccttctt
900 gagcagctcc ttgaccttgt tggcaacaag tctccgacct cggaggtgga
ggaagagcct 960 ccgatatcgg cggtagtgat accagcctcg acggactcct
tgacggcagc ctcaacagcg 1020 tcaccggcgg gcttcatgtt aagagagaac
ttgagcatca tggcggcaga cagaatggtg 1080 gcaatggggt tgaccttctg
cttgccgaga tcgggggcag atccgtgaca gggctcgtac 1140 agaccgaacg
cctcgttggt gtcgggcaga gaagccagag aggcggaggg cagcagaccc 1200
agagaaccgg ggatgacgga ggcctcgtcg gagatgatat cgccaaacat gttggtggtg
1260 atgatgatac cattcatctt ggagggctgc ttgatgagga tcatggcggc
cgagtcgatc 1320 agctggtggt tgagctcgag ctgggggaat tcgtccttga
ggactcgagt gacagtcttt 1380 cgccaaagtc gagaggaggc cagcacgttg
gccttgtcaa gagaccacac gggaagaggg 1440 gggttgtgct gaagggccag
gaaggcggcc attcgggcaa ttcgctcaac ctcaggaacg 1500 gagtaggtct
cggtgtcgga agcgacgcca gatccgtcat cctcctttcg ctctccaaag 1560
tagatacctc cgacgagctc tcggacaatg atgaagtcgg tgccctcaac gtttcggatg
1620 ggggagagat cggcgagctt gggcgacagc agctggcagg gtcgcaggtt
ggcgtacagg 1680 ttcaggtcct ttcgcagctt gaggagaccc tgctcgggtc
gcacgtcggt tcgtccgtcg 1740 ggagtggtcc atacggtgtt ggcagcgcct
ccgacagcac cgagcataat agagtcagcc 1800 tttcggcaga tgtcgagagt
agcgtcggtg atgggctcgc cctccttctc aatggcagct 1860 cctccaatga
gtcggtcctc aaacacaaac tcggtgccgg aggcctcagc aacagacttg 1920
agcaccttga cggcctcggc aatcacctcg gggccacaga agtcgccgcc gagaagaaca
1980 atcttcttgg agtcagtctt ggtcttctta gtttcgggtt ccattgtgga
tgtgtgtggt 2040 tgtatgtgtg atgtggtgtg tggagtgaaa atctgtggct
ggcaaacgct cttgtatata 2100 tacgcacttt tgcccgtgct atgtggaaga
ctaaacctcc gaagattgtg actcaggtag 2160 tgcggtatcg gctagggacc
caaaccttgt cgatgccgat agcgctatcg aacgtacccc 2220 agccggccgg
gagtatgtcg gaggggacat acgagatcgt caagggtttg tggccaactg 2280
gtatttaaat gatgtcgact catcgatata acttcgtata atgtatgcta tacgaagtta
2340 tcctaggtat agatctgtta ccggacagaa gtaccccaag ctcaacaaat
gggctgtcaa 2400 ccacttcaac cccaacgccc cgctgtttga gaagaaggac
tggttcaaca tctggatctc 2460 taacgtcggt attggtatca ccatgtccgt
catcgcatac tccatcaacc gatggggcct 2520 ggcttccgtc accctctact
acctgatccc ctacctgtgg gtcaaccact ggctcgtggc 2580 catcacctac
ctgcagcaca ccgaccccac tctgccccac taccacgccg accagtggaa 2640
cttcacccga ggagccgccg ccaccatcga ccgagagttt ggcttcatcg gctccttctg
2700 cttccatgac atcatcgaga cccacgttct gcaccactac gtgtctcgaa
ttcccttcta 2760 caacgcccga atcgccactg agaagatcaa gaaggtcatg
ggcaagcact accgacacga 2820 cgacaccaac ttcatcaagt ctctttacac
tgtcgcccga acctgccagt ttgttgaagg 2880 taaggaaggc attcagatgt
ttagaaacgt caatggagtc ggagttgctc ctgacggcct 2940 gccttctaaa
ggcgcgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3000
tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
3060 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
gaatcagggg 3120 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
ggccaggaac cgtaaaaagg 3180 ccgcgttgct ggcgtttttc cataggctcc
gcccccctga cgagcatcac aaaaatcgac 3240 gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg 3300 gaagctccct
cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3360
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
3420 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
cccgaccgct 3480 gcgccttatc cggtaactat cgtcttgagt ccaacccggt
aagacacgac ttatcgccac 3540 tggcagcagc cactggtaac aggattagca
gagcgaggta tgtaggcggt gctacagagt 3600 tcttgaagtg gtggcctaac
tacggctaca ctagaagaac agtatttggt atctgcgctc 3660 tgctgaagcc
agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3720
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3780 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
gaaaactcac 3840 gttaagggat tttggtcatg agattatcaa aaaggatctt
cacctagatc cttttaaatt 3900 aaaaatgaag ttttaaatca atctaaagta
tatatgagta aacttggtct gacagttacc 3960 aatgcttaat cagtgaggca
cctatctcag cgatctgtct atttcgttca tccatagttg 4020 cctgactccc
cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4080
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
4140 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
atccagtcta 4200 ttaattgttg ccgggaagct agagtaagta gttcgccagt
taatagtttg cgcaacgttg 4260 ttgccattgc tacaggcatc gtggtgtcac
gctcgtcgtt tggtatggct tcattcagct 4320 ccggttccca acgatcaagg
cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4380 gctccttcgg
tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4440
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
4500 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
agttgctctt 4560 gcccggcgtc aatacgggat aataccgcgc cacatagcag
aactttaaaa gtgctcatca 4620 ttggaaaacg ttcttcgggg cgaaaactct
caaggatctt accgctgttg agatccagtt 4680 cgatgtaacc cactcgtgca
cccaactgat cttcagcatc ttttactttc accagcgttt 4740 ctgggtgagc
aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4800
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
4860 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
ggggttccgc 4920 gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa
taccgcacag atgcgtaagg 4980 agaaaatacc gcatcaggaa attgtaagcg
ttaatatttt gttaaaattc gcgttaaatt 5040 tttgttaaat cagctcattt
tttaaccaat aggccgaaat cggcaaaatc ccttataaat 5100 caaaagaata
gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 5160
taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
5220 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
gcactaaatc 5280 ggaaccctaa agggagcccc cgatttagag cttgacgggg
aaagccggcg aacgtggcga 5340 gaaaggaagg gaagaaagcg aaaggagcgg
gcgctagggc gctggcaagt gtagcggtca 5400 cgctgcgcgt aaccaccaca
cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc 5460 gccattcagg
ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 5520
ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
5580 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
tatagggcga 5640 attgggcccg acgtcgcatg catggattcg accacgcaga
ccaacaccgg caccggcaag 5700 gtggccgtgc agccccccac ggccttcatt
aagcccattg agaaggtgtc cgagcccgtc 5760 tacgacacct ttggcaacga
gttcactcct ccagactact ctatcaagga tattctggat 5820 gccattcccc
aggagtgcta caagcggtcc tacgttaagt cctactcgta cgtggcccga 5880
gactgcttct ttatcgccgt ttttgcctac atggcctacg cgtacctgcc tcttattccc
5940 tcggcttccg gccgagctgt ggcctgggcc atgtactcca ttgtccaggg
tctgtttggc 6000 accggtctgt gggttcttgc ccacgagtgt ggccactctg
ctttctccga ctctaacacc 6060 gtcaacaacg tcaccggatg ggttctgcac
tcctccatgc tggtccctta ctacgcctgg 6120 aagctgaccc actccatgca
ccacaagtcc actggtcacc tcacccgtga tatggtgttt 6180 gtgcccaagg
accgaaagga gtttatggag aaccgaggcg cccatgactg gtctgagctt 6240
gctgaggacg ctcccctcat gattaat 6267 <210> SEQ ID NO 94
<211> LENGTH: 9570 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Plasmid pY117 <400> SEQUENCE: 94
ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt
60 ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta
acctcccaca 120 ctcctttgac ataacgattt atgtaacgaa actgaaattt
gaccagatat tgtgtccgcg 180 gtggagctcc agcttttgtt ccctttagtg
agggtttaaa cgagcttggc gtaatcatgg 240 tcatagctgt ttcctgtgtg
aaattgttat ccgctcacaa ttccacacaa cgtacgagcc 300 ggaagcataa
agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 360
ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
420 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
ctcgctcact 480 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
cagctcactc aaaggcggta 540 atacggttat ccacagaatc aggggataac
gcaggaaaga acatgtgagc aaaaggccag 600 caaaaggcca ggaaccgtaa
aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 660 cctgacgagc
atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 720
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
780 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
ttctcatagc 840 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
ccaagctggg ctgtgtgcac 900 gaaccccccg ttcagcccga ccgctgcgcc
ttatccggta actatcgtct tgagtccaac 960 ccggtaagac acgacttatc
gccactggca gcagccactg gtaacaggat tagcagagcg 1020 aggtatgtag
gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 1080
aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
1140 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
ttgcaagcag 1200 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
tgatcttttc tacggggtct 1260 gacgctcagt ggaacgaaaa ctcacgttaa
gggattttgg tcatgagatt atcaaaaagg 1320 atcttcacct agatcctttt
aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1380 gagtaaactt
ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 1440
tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
1500 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
ctcaccggct 1560 ccagatttat cagcaataaa ccagccagcc ggaagggccg
agcgcagaag tggtcctgca 1620 actttatccg cctccatcca gtctattaat
tgttgccggg aagctagagt aagtagttcg 1680 ccagttaata gtttgcgcaa
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1740 tcgtttggta
tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 1800
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
1860 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
tactgtcatg 1920 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
ccaagtcatt ctgagaatag 1980 tgtatgcggc gaccgagttg ctcttgcccg
gcgtcaatac gggataatac cgcgccacat 2040 agcagaactt taaaagtgct
catcattgga aaacgttctt cggggcgaaa actctcaagg 2100 atcttaccgc
tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 2160
gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
2220 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
ttttcaatat 2280 tattgaagca tttatcaggg ttattgtctc atgagcggat
acatatttga atgtatttag 2340 aaaaataaac aaataggggt tccgcgcaca
tttccccgaa aagtgccacc tgacgcgccc 2400 tgtagcggcg cattaagcgc
ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2460 gccagcgccc
tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 2520
ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
2580 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
gccatcgccc 2640 tgatagacgg tttttcgccc tttgacgttg gagtccacgt
tctttaatag tggactcttg 2700 ttccaaactg gaacaacact caaccctatc
tcggtctatt cttttgattt ataagggatt 2760 ttgccgattt cggcctattg
gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 2820 tttaacaaaa
tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg 2880
aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
2940 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
aaaacgacgg 3000 ccagtgaatt gtaatacgac tcactatagg gcgaattggg
taccgggccc cccctcgagg 3060 tcgatggtgt cgataagctt gatatcgaat
tcatgtcaca caaaccgatc ttcgcctcaa 3120 ggaaacctaa ttctacatcc
gagagactgc cgagatccag tctacactga ttaattttcg 3180 ggccaataat
ttaaaaaaat cgtgttatat aatattatat gtattatata tatacatcat 3240
gatgatactg acagtcatgt cccattgcta aatagacaga ctccatctgc cgcctccaac
3300 tgatgttctc aatatttaag gggtcatctc gcattgttta ataataaaca
gactccatct 3360 accgcctcca aatgatgttc tcaaaatata ttgtatgaac
ttatttttat tacttagtat 3420 tattagacaa cttacttgct ttatgaaaaa
cacttcctat ttaggaaaca atttataatg 3480 gcagttcgtt catttaacaa
tttatgtaga ataaatgtta taaatgcgta tgggaaatct 3540 taaatatgga
tagcataaat gatatctgca ttgcctaatt cgaaatcaac agcaacgaaa 3600
aaaatccctt gtacaacata aatagtcatc gagaaatatc aactatcaaa gaacagctat
3660 tcacacgtta ctattgagat tattattgga cgagaatcac acactcaact
gtctttctct 3720 cttctagaaa tacaggtaca agtatgtact attctcattg
ttcatacttc tagtcatttc 3780 atcccacata ttccttggat ttctctccaa
tgaatgacat tctatcttgc aaattcaaca 3840 attataataa gatataccaa
agtagcggta tagtggcaat caaaaagctt ctctggtgtg 3900 cttctcgtat
ttatttttat tctaatgatc cattaaaggt atatatttat ttcttgttat 3960
ataatccttt tgtttattac atgggctgga tacataaagg tattttgatt taattttttg
4020 cttaaattca atcccccctc gttcagtgtc aactgtaatg gtaggaaatt
accatacttt 4080 tgaagaagca aaaaaaatga aagaaaaaaa aaatcgtatt
tccaggttag acgttccgca 4140 gaatctagaa tgcggtatgc ggtacattgt
tcttcgaacg taaaagttgc gctccctgag 4200 atattgtaca tttttgcttt
tacaagtaca agtacatcgt acaactatgt actactgttg 4260
atgcatccac aacagtttgt tttgtttttt tttgtttttt ttttttctaa tgattcatta
4320 ccgctatgta tacctacttg tacttgtagt aagccgggtt attggcgttc
aattaatcat 4380 agacttatga atctgcacgg tgtgcgctgc gagttacttt
tagcttatgc atgctacttg 4440 ggtgtaatat tgggatctgt tcggaaatca
acggatgctc aaccgatttc gacagtaatt 4500 aattaattcc ctagtcccag
tgtacacccg ccgatatcgc ttaccctgca gccggattaa 4560 ggttggcaat
ttttcacgtc cttgtctccg caattactca ccgggtggtt tataagattg 4620
caagcgtctt gatttgtctc tgtatactaa catgcaatcg cgactcgccc gacgggccac
4680 taacctggcc agaatctcca gatccaagta ttctcttggt ctgcgatatg
tttccaacac 4740 aaaagcccct gctgcccagc cggcaactgc tgagtgagta
ttccttgcca taaacgaccc 4800 agaaccactg tatagtgttt ggaagcacta
gtcagaagac cagcgaaaac aggtggaaaa 4860 aactgagacg aaaagcaacg
accagaaatg taatgtgtgg aaaagcgaca cacacagagc 4920 agataaagag
gtgacaaata acgacaaatg aaatatcagt atcttcccac aatcactacc 4980
tctcagctgt ctgaaggtgc ggctgatata tccatcccac gtctaacgta tggagtgtga
5040 tagaatatga cgacacaagc atgagaactc gctctctatc caaccaccga
aacactgtca 5100 ctacagccgt tcttgttgct ccattcgctt ttgtgattcc
atgccttctc tggtgactga 5160 caacattcct tccttttctc cagccctgtt
gttatctgct catgacctac ggccactctc 5220 tatcgcatac taacatagac
gatcccagcc cgctccccac ttccagggca ccgttggcaa 5280 gcctcctatc
ctcaagaagg ctgaggctgc caacgctgac atggacgagt ccttcatcgg 5340
aatgtctgga ggagagatct tccacgagat gatgctgcga cacaacgtcg acactgtctt
5400 cggttacccc ggtggagcca ttctccccgt ctttgacgcc attcacaact
ctgagtactt 5460 caactttgtg ctccctcgac acgagcaggg tgccggccac
atggccgagg gctacgctcg 5520 agcctctggt aagcccggtg tcgttctcgt
cacctctggc cccggtgcca ccaacgtcat 5580 cacccccatg caggacgctc
tttccgatgg tacccccatg gttgtcttca ccggtcaggt 5640 cctgacctcc
gttatcggca ctgacgcctt ccaggaggcc gatgttgtcg gcatctcccg 5700
atcttgcacc aagtggaacg tcatggtcaa gaacgttgct gagctccccc gacgaatcaa
5760 cgaggccttt gagattgcta cttccggccg acccggtccc gttctcgtcg
atctgcccaa 5820 ggatgttact gctgccatcc tgcgagagcc catccccacc
aagtccacca ttccctcgca 5880 ttctctgacc aacctcacct ctgccgccgc
caccgagttc cagaagcagg ctatccagcg 5940 agccgccaac ctcatcaacc
agtccaagaa gcccgtcctt tacgtcggac agggtatcct 6000 tggctccgag
gagggtccta agctgcttaa ggagctggct gagaaggccg agattcccgt 6060
caccactact ctgcagggtc ttggtgcctt tgacgagcga gaccccaagt ctctgcacat
6120 gctcggtatg cacggttccg gctacgccaa catggccatg cagaacgctg
actgtatcat 6180 tgctctcggc gcccgatttg atgaccgagt taccggctcc
atccccaagt ttgcccccga 6240 ggctcgagcc gctgcccttg agggtcgagg
tggtattgtt cactttgaga tccaggccaa 6300 gaacatcaac aaggttgttc
aggccaccga agccgttgag ggagacgtta ccgagtctgt 6360 ccgacagctc
atccccctca tcaacaaggt ctctgccgct gagcgagctc cctggactga 6420
gactatccag tcctggaagc agcagttccc cttcctcttc gaggctgaag gtgaggatgg
6480 tgttatcaag ccccagtccg tcattgctct gctctctgac ctgacagaga
acaacaagga 6540 caagaccatc atcaccaccg gtgttggtca gcatcagatg
tggactgccc agcatttccg 6600 atggcgacac cctcgaacca tgatcacttc
tggtggtctt ggaactatgg gttacggcct 6660 gcccgccgct atcggcgcca
aggttgcccg acctgactgc gacgtcattg acatcgatgg 6720 tgacgcttct
ttcaacatga ctctgaccga gctgtccacc gccgttcagt tcaacattgg 6780
cgtcaaggct attgtcctca acaacgagga acagggtatg gtcacccagc tgcagtctct
6840 cttctacgag aaccgatact gccacactca tcagaagaac cccgacttca
tgaagctggc 6900 cgagtccatg ggcatgaagg gtatccgaat cactcacatt
gaccagctgg aggccggtct 6960 caaggagatg ctcgcataca agggccctgt
gctcgttgag gttgttgtcg acaagaagat 7020 ccccgttctt cccatggttc
ccgctggtaa ggctttgcat gagttccttg tctacgacgc 7080 tgacgccgag
gctgcttctc gacccgatcg actgaagaat gcccccgccc ctcacgtcca 7140
ccagaccacc tttgagaact aagtggaaag gaacacaagc aatccgaacc aaaaataatt
7200 ggggtcccgt gcccacagag tctagtgcag acctaaaatg accacagtaa
attatagctg 7260 ttattaaaca tgagattttg accaacaaga gcgtaggaat
gttattagct actacttgta 7320 catacacagc atttgtttta aataatgttg
cctccagggg cagtgagatc aggacccaga 7380 tccgtggcca gctctctgac
ttcagaccgc ttgtacttaa gcagctcgca acactgttgt 7440 cgaggattga
acttgccata ttcgattttg tggtcatgaa tccagcacac ctcatttaaa 7500
tgtagctaac ggtagcaggc gaactactgg tacatacctc ccccggaata tgtacaggca
7560 taatgcgtat ctgtgggaca tgtggtcgtt gcgccattat gtaagcagcg
tgtactcctc 7620 tgactgtcca tatggtttgc tccatctcac cctcatcgtt
ttcattgttc acaggcggcc 7680 acaaaaaaac tgtcttctct ccttctctct
tcgccttagt ctactcggac cagttttagt 7740 ttagcttggc gccactggat
aaatgagacc tcaggccttg tgatgaggag gtcacttatg 7800 aagcatgtta
ggaggtgctt gtatggatag agaagcaccc aaaataataa gaataataat 7860
aaaacagggg gcgttgtcat ttcatatcgt gttttcacca tcaatacacc tccaaacaat
7920 gcccttcatg tggccagccc caatattgtc ctgtagttca actctatgca
gctcgtatct 7980 tattgagcaa gtaaaactct gtcagccgat attgcccgac
ccgcgacaag ggtcaacaag 8040 gtggtgtaag gccttcgcag aagtcaaaac
tgtgccaaac aaacatctag agtctctttg 8100 gtgtttctcg catatatttw
atcggctgtc ttacgtattt gcgcctcggt accggactaa 8160 tttcggatca
tccccaatac gctttttctt cgcagctgtc aacagtgtcc atgatctatc 8220
cacctaaatg ggtcatatga ggcgtataat ttcgtggtgc tgataataat tcccatatat
8280 ttgacacaaa acttcccccc ctagacatac atctcacaat ctcacttctt
gtgcttctgt 8340 cacacatctc ctccagctga cttcaactca cacctctgcc
ccagttggtc tacagcggta 8400 taaggtttct ccgcatagag gtgcaccact
cctcccgata cttgtttgtg tgacttgtgg 8460 gtcacgacat atatatctac
acacattgcg ccaccctttg gttcttccag cacaacaaaa 8520 acacgacacg
ctaaccatgg ccaatttact gaccgtacac caaaatttgc ctgcattacc 8580
ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
8640 ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
gggcggcatg 8700 gtgcaagttg aataaccgga aatggtttcc cgcagaacct
gaagatgttc gcgattatct 8760 tctatatctt caggcgcgcg gtctggcagt
aaaaactatc cagcaacatt tgggccagct 8820 aaacatgctt catcgtcggt
ccgggctgcc acgaccaagt gacagcaatg ctgtttcact 8880 ggttatgcgg
cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct 8940
agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg
9000 ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt
tacgtatagc 9060 cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
gacggtggga gaatgttaat 9120 ccatattggc agaacgaaaa cgctggttag
caccgcaggt gtagagaagg cacttagcct 9180 gggggtaact aaactggtcg
agcgatggat ttccgtctct ggtgtagctg atgatccgaa 9240 taactacctg
ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca 9300
gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc
9360 taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
gtgtcggagc 9420 cgcgcgagat atggcccgcg ctggagtttc aataccggag
atcatgcaag ctggtggctg 9480 gaccaatgta aatattgtca tgaactatat
ccgtaacctg gatagtgaaa caggggcaat 9540 ggtgcgcctg ctggaagatg
gcgattaagc 9570 <210> SEQ ID NO 95 <211> LENGTH: 358
<212> TYPE: PRT <213> ORGANISM: Saprolegnia diclina
<400> SEQUENCE: 95 Met Thr Glu Asp Lys Thr Lys Val Glu Phe
Pro Thr Leu Thr Glu Leu 1 5 10 15 Lys His Ser Ile Pro Asn Ala Cys
Phe Glu Ser Asn Leu Gly Leu Ser 20 25 30 Leu Tyr Tyr Thr Ala Arg
Ala Ile Phe Asn Ala Ser Ala Ser Ala Ala 35 40 45 Leu Leu Tyr Ala
Ala Arg Ser Thr Pro Phe Ile Ala Asp Asn Val Leu 50 55 60 Leu His
Ala Leu Val Cys Ala Thr Tyr Ile Tyr Val Gln Gly Val Ile 65 70 75 80
Phe Trp Gly Phe Phe Thr Val Gly His Asp Cys Gly His Ser Ala Phe 85
90 95 Ser Arg Tyr His Ser Val Asn Phe Ile Ile Gly Cys Ile Met His
Ser 100 105 110 Ala Ile Leu Thr Pro Phe Glu Ser Trp Arg Val Thr His
Arg His His 115 120 125 His Lys Asn Thr Gly Asn Ile Asp Lys Asp Glu
Ile Phe Tyr Pro His 130 135 140 Arg Ser Val Lys Asp Leu Gln Asp Val
Arg Gln Trp Val Tyr Thr Leu 145 150 155 160 Gly Gly Ala Trp Phe Val
Tyr Leu Lys Val Gly Tyr Ala Pro Arg Thr 165 170 175 Met Ser His Phe
Asp Pro Trp Asp Pro Leu Leu Leu Arg Arg Ala Ser 180 185 190 Ala Val
Ile Val Ser Leu Gly Val Trp Ala Ala Phe Phe Ala Ala Tyr 195 200 205
Ala Tyr Leu Thr Tyr Ser Leu Gly Phe Ala Val Met Gly Leu Tyr Tyr 210
215 220 Tyr Ala Pro Leu Phe Val Phe Ala Ser Phe Leu Val Ile Thr Thr
Phe 225 230 235 240 Leu His His Asn Asp Glu Ala Thr Pro Trp Tyr Gly
Asp Ser Glu Trp 245 250 255 Thr Tyr Val Lys Gly Asn Leu Ser Ser Val
Asp Arg Ser Tyr Gly Ala 260 265 270 Phe Val Asp Asn Leu Ser His His
Ile Gly Thr His Gln Val His His 275 280 285 Leu Phe Pro Ile Ile Pro
His Tyr Lys Leu Asn Glu Ala Thr Lys His 290 295 300 Phe Ala Ala Ala
Tyr Pro His Leu Val Arg Arg Asn Asp Glu Pro Ile 305 310 315 320 Ile
Thr Ala Phe Phe Lys Thr Ala His Leu Phe Val Asn Tyr Gly Ala 325 330
335 Val Pro Glu Thr Ala Gln Ile Phe Thr Leu Lys Glu Ser Ala Ala Ala
340 345 350
Ala Lys Ala Lys Ser Asp 355 <210> SEQ ID NO 96 <211>
LENGTH: 9 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Delta-17 Desaturase Motif #1 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (3)..(3) <223>
OTHER INFORMATION: Xaa can be any naturally occurring amino acid
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (7)..(7) <223> OTHER INFORMATION: Xaa can be any
naturally occurring amino acid <400> SEQUENCE: 96 Phe Thr Xaa
Gly His Asp Xaa Gly His 1 5 <210> SEQ ID NO 97 <211>
LENGTH: 9 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Delta-17 Desaturase Motif #2 <400> SEQUENCE: 97 His Arg His
His His Lys Asn Thr Gly 1 5 <210> SEQ ID NO 98 <211>
LENGTH: 11 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Delta-17 Desaturase Motif #3 <220> FEATURE: <221>
NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223>
OTHER INFORMATION: Xaa can be any naturally occurring amino acid
<400> SEQUENCE: 98 Ile Gly Thr His Gln Xaa His His Leu Phe
Pro 1 5 10 <210> SEQ ID NO 99 <211> LENGTH: 5
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: His-rich motif
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(4) <223> OTHER INFORMATION: Xaa can be any
naturally occurring amino acid <400> SEQUENCE: 99 His Xaa Xaa
Xaa His 1 5 <210> SEQ ID NO 100 <211> LENGTH: 5
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: His-rich motif
<220> FEATURE: <221> NAME/KEY: misc_feature <222>
LOCATION: (2)..(3) <223> OTHER INFORMATION: Xaa can be any
naturally occurring amino acid <400> SEQUENCE: 100 His Xaa
Xaa His His 1 5 <210> SEQ ID NO 101 <211> LENGTH: 5
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: His-rich motif
<220> FEATURE: <221> NAME/KEY: MISC_FEATURE <222>
LOCATION: (1)..(1) <223> OTHER INFORMATION: Xaa = His [H] or
Gln [Q] <220> FEATURE: <221> NAME/KEY: misc_feature
<222> LOCATION: (2)..(3) <223> OTHER INFORMATION: Xaa
can be any naturally occurring amino acid <400> SEQUENCE: 101
Xaa Xaa Xaa His His 1 5 <210> SEQ ID NO 102 <211>
LENGTH: 8067 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Plasmid pFBAINPaD17S <400> SEQUENCE: 102 ggccgcaagt
gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa 60
gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac
120 ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag
tacaatacta 180 aacatactgt acatactcat actcgtaccc gggcaacggt
ttcacttgag tgcagtggct 240 agtgctctta ctcgtacagt gtgcaatact
gcgtatcata gtctttgatg tatatcgtat 300 tcattcatgt tagttgcgta
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 360 gagtgagcta
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 420
tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
480 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
ctgcggcgag 540 cggtatcagc tcactcaaag gcggtaatac ggttatccac
agaatcaggg gataacgcag 600 gaaagaacat gtgagcaaaa ggccagcaaa
aggccaggaa ccgtaaaaag gccgcgttgc 660 tggcgttttt ccataggctc
cgcccccctg acgagcatca caaaaatcga cgctcaagtc 720 agaggtggcg
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 780
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
840 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
gtgtaggtcg 900 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
gcccgaccgc tgcgccttat 960 ccggtaacta tcgtcttgag tccaacccgg
taagacacga cttatcgcca ctggcagcag 1020 ccactggtaa caggattagc
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 1080 ggtggcctaa
ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 1140
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
1200 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag 1260 atcctttgat cttttctacg gggtctgacg ctcagtggaa
cgaaaactca cgttaaggga 1320 ttttggtcat gagattatca aaaaggatct
tcacctagat ccttttaaat taaaaatgaa 1380 gttttaaatc aatctaaagt
atatatgagt aaacttggtc tgacagttac caatgcttaa 1440 tcagtgaggc
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1500
ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
1560 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa 1620 gggccgagcg cagaagtggt cctgcaactt tatccgcctc
catccagtct attaattgtt 1680 gccgggaagc tagagtaagt agttcgccag
ttaatagttt gcgcaacgtt gttgccattg 1740 ctacaggcat cgtggtgtca
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1800 aacgatcaag
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1860
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
1920 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
actggtgagt 1980 actcaaccaa gtcattctga gaatagtgta tgcggcgacc
gagttgctct tgcccggcgt 2040 caatacggga taataccgcg ccacatagca
gaactttaaa agtgctcatc attggaaaac 2100 gttcttcggg gcgaaaactc
tcaaggatct taccgctgtt gagatccagt tcgatgtaac 2160 ccactcgtgc
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 2220
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
2280 tactcatact cttccttttt caatattatt gaagcattta tcagggttat
tgtctcatga 2340 gcggatacat atttgaatgt atttagaaaa ataaacaaat
aggggttccg cgcacatttc 2400 cccgaaaagt gccacctgac gcgccctgta
gcggcgcatt aagcgcggcg ggtgtggtgg 2460 ttacgcgcag cgtgaccgct
acacttgcca gcgccctagc gcccgctcct ttcgctttct 2520 tcccttcctt
tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 2580
ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
2640 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
acgttggagt 2700 ccacgttctt taatagtgga ctcttgttcc aaactggaac
aacactcaac cctatctcgg 2760 tctattcttt tgatttataa gggattttgc
cgatttcggc ctattggtta aaaaatgagc 2820 tgatttaaca aaaatttaac
gcgaatttta acaaaatatt aacgcttaca atttccattc 2880 gccattcagg
ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 2940
ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
3000 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
tatagggcga 3060 attgggtacc gggccccccc tcgaggtcga tggtgtcgat
aagcttgata tcgaattcat 3120 gtcacacaaa ccgatcttcg cctcaaggaa
acctaattct acatccgaga gactgccgag 3180 atccagtcta cactgattaa
ttttcgggcc aataatttaa aaaaatcgtg ttatataata 3240 ttatatgtat
tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata 3300
gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat
3360 tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa
aatatattgt 3420 atgaacttat ttttattact tagtattatt agacaactta
cttgctttat gaaaaacact 3480 tcctatttag gaaacaattt ataatggcag
ttcgttcatt taacaattta tgtagaataa 3540 atgttataaa tgcgtatggg
aaatcttaaa tatggatagc ataaatgata tctgcattgc 3600 ctaattcgaa
atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga 3660
aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag
3720 aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta
tgtactattc 3780
tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa
3840 tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta
gcggtatagt 3900 ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat
ttttattcta atgatccatt 3960 aaaggtatat atttatttct tgttatataa
tccttttgtt tattacatgg gctggataca 4020 taaaggtatt ttgatttaat
tttttgctta aattcaatcc cccctcgttc agtgtcaact 4080 gtaatggtag
gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat 4140
cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt
4200 cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca
agtacaagta 4260 catcgtacaa ctatgtacta ctgttgatgc atccacaaca
gtttgttttg tttttttttg 4320 tttttttttt ttctaatgat tcattaccgc
tatgtatacc tacttgtact tgtagtaagc 4380 cgggttattg gcgttcaatt
aatcatagac ttatgaatct gcacggtgtg cgctgcgagt 4440 tacttttagc
ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg 4500
atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc
4560 ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc
cgtatcgaga 4620 aacacaacaa catgccccat tggacagatc atgcggatac
acaggttgtg cagtatcata 4680 catactcgat cagacaggtc gtctgaccat
catacaagct gaacaagcgc tccatacttg 4740 cacgctctct atatacacag
ttaaattaca tatccatagt ctaacctcta acagttaatc 4800 ttctggtaag
cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg 4860
ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc
4920 tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc
caccccgggg 4980 gtcagaataa gccagtcctc agagtcgccc ttaggtcggt
tctgggcaat gaagccaacc 5040 acaaactcgg ggtcggatcg ggcaagctca
atggtctgct tggagtactc gccagtggcc 5100 agagagccct tgcaagacag
ctcggccagc atgagcagac ctctggccag cttctcgttg 5160 ggagagggga
ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc 5220
ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg
5280 ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca
ccggtactgg 5340 tgcttgacag tgttgccaat atctgcgaac tttctgtcct
cgaacaggaa gaaaccgtgc 5400 ttaagagcaa gttccttgag ggggagcaca
gtgccggcgt aggtgaagtc gtcaatgatg 5460 tcgatatggg ttttgatcat
gcacacataa ggtccgacct tatcggcaag ctcaatgagc 5520 tccttggtgg
tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc 5580
ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc
5640 attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
tatcggaacc 5700 ttatctgggg cagtgaagta tatgttatgg taatagttac
gagttagttg aacttataga 5760 tagactggac tatacggcta tcggtccaaa
ttagaaagaa cgtcaatggc tctctgggcg 5820 tcgcctttgc cgacaaaaat
gtgatcatga tgaaagccag caatgacgtt gcagctgata 5880 ttgttgtcgg
ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa 5940
tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat
6000 gacgagtcag acagatactc gtcgaaaaca gtgtacgcag atctactata
gaggaacatt 6060 taaattgccc cggagaagac ggccaggccg cctagatgac
aaattcaaca actcacagct 6120 gactttctgc cattgccact aggggggggc
ctttttatat ggccaagcca agctctccac 6180 gtcggttggg ctgcacccaa
caataaatgg gtagggttgc accaacaaag ggatgggatg 6240 gggggtagaa
gatacgagga taacggggct caatggcaca aataagaacg aatactgcca 6300
ttaagactcg tgatccagcg actgacacca ttgcatcatc taagggcctc aaaactacct
6360 cggaactgct gcgctgatct ggacaccaca gaggttccga gcactttagg
ttgcaccaaa 6420 tgtcccacca ggtgcaggca gaaaacgctg gaacagcgtg
tacagtttgt cttaacaaaa 6480 agtgagggcg ctgaggtcga gcagggtggt
gtgacttgtt atagccttta gagctgcgaa 6540 agcgcgtatg gatttggctc
atcaggccag attgagggtc tgtggacaca tgtcatgtta 6600 gtgtacttca
atcgccccct ggatatagcc ccgacaatag gccgtggcct catttttttg 6660
ccttccgcac atttccattg ctcggtaccc acaccttgct tctcctgcac ttgccaacct
6720 taatactggt ttacattgac caacatctta caagcggggg gcttgtctag
ggtatatata 6780 aacagtggct ctcccaatcg gttgccagtc tcttttttcc
tttctttccc cacagattcg 6840 aaatctaaac tacacatcac agaattccga
gccgtgagta tccacgacaa gatcagtgtc 6900 gagacgacgc gttttgtgta
atgacacaat ccgaaagtcg ctagcaacac acactctcta 6960 cacaaactaa
cccagctctg gtaccatggc ttcctctacc gttgccgctc cctacgagtt 7020
ccctactctc accgagatca agcgatccct gcctgcccac tgcttcgaag cctctgttcc
7080 ctggtccctc tactataccg tgcgagctct gggcattgcc ggttcccttg
ctctcggact 7140 gtactatgct cgagcccttg ctatcgtgca ggagtttgca
ctgctcgatg ccgtcctttg 7200 cactggctac attctgctcc agggtatcgt
gttctgggga ttctttacca tcggtcacga 7260 ctgtggacat ggtgccttct
cgcgatccca cctgctcaac ttctctgttg gcacactcat 7320 tcactccatc
attctgactc cctacgagtc gtggaagatc agccatcgac accatcacaa 7380
gaacaccggc aacatcgaca aggatgagat cttctaccct cagcgagaag ccgactctca
7440 tcccctgtcc cgacacatgg tcatctccct tggttcggct tggtttgcct
acctcgttgc 7500 tggatttcct ccccgaaagg tcaaccactt caatccctgg
gagcctctct acctgcgaag 7560 aatgtctgcc gtcatcattt ccctcggctc
tctcgtggcc tttgctggtc tgtacgccta 7620 ccttacctac gtctacggcc
tcaagaccat ggctctgtat tacttcgcac ctctctttgg 7680 attcgccacc
atgctggttg tcactacctt cctccatcac aacgacgagg aaactccctg 7740
gtacgccgat tcggagtgga cctatgtcaa gggcaacttg tcctctgtgg accgaagcta
7800 cggagccctc atcgacaacc tgtcccacaa cattggtaca catcagatcc
accatctgtt 7860 tcccatcatt cctcactaca agctcaacga ggccactgct
gccttcgctc aggcctttcc 7920 cgaactggtg cgaaagtcgg cttctcccat
cattcccacc ttcatccgaa ttggtcttat 7980 gtacgccaag tacggcgtgg
tcgacaagga tgccaagatg tttaccctca aggaggccaa 8040 ggctgccaag
accaaagcca actaagc 8067
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