U.S. patent application number 13/513757 was filed with the patent office on 2012-11-29 for microrna and use thereof in identification of b cell malignancies.
Invention is credited to Sandeep Dave, Cassandra Jacobs.
Application Number | 20120302626 13/513757 |
Document ID | / |
Family ID | 44115523 |
Filed Date | 2012-11-29 |
United States Patent
Application |
20120302626 |
Kind Code |
A1 |
Dave; Sandeep ; et
al. |
November 29, 2012 |
MICRORNA AND USE THEREOF IN IDENTIFICATION OF B CELL
MALIGNANCIES
Abstract
Disclosed are nucleic acid sequences, including microRNA
sequences and cDNA sequences, as well as vectors, DNA libraries,
microarrays, and recombinant cells comprising the nucleic acid
sequences described herein. Methods of determining the B cell stage
from which a B cell malignancy is derived. Methods of identifying B
cell malignancies are also provided. Methods of diagnosing B cell
malignancies are provided. Such methods comprise, in certain
embodiments, detecting one or more microRNAs or cDNAs as disclosed
herein.
Inventors: |
Dave; Sandeep; (Chapel Hill,
NC) ; Jacobs; Cassandra; (Durham, NC) |
Family ID: |
44115523 |
Appl. No.: |
13/513757 |
Filed: |
December 3, 2010 |
PCT Filed: |
December 3, 2010 |
PCT NO: |
PCT/US10/58952 |
371 Date: |
August 13, 2012 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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61266733 |
Dec 4, 2009 |
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Current U.S.
Class: |
514/44A ;
435/243; 435/320.1; 435/6.12; 435/6.14; 506/9; 536/23.1;
536/24.5 |
Current CPC
Class: |
C12Q 2600/16 20130101;
C12Q 2600/112 20130101; C12Q 2600/178 20130101; C12Q 1/6886
20130101; C12Q 2600/158 20130101 |
Class at
Publication: |
514/44.A ;
536/23.1; 536/24.5; 435/320.1; 435/6.14; 435/6.12; 435/243;
506/9 |
International
Class: |
C12N 15/113 20100101
C12N015/113; A61K 31/7088 20060101 A61K031/7088; C40B 30/04
20060101 C40B030/04; C12Q 1/68 20060101 C12Q001/68; C12N 1/00
20060101 C12N001/00; C12N 15/11 20060101 C12N015/11; C12N 15/63
20060101 C12N015/63 |
Goverment Interests
STATEMENT OF GOVERNMENT SUPPORT
[0002] This invention was made with United States government
support from the National Institutes of Health grant no.
K12-CA-100639. The United States government has certain rights in
this invention.
Claims
1. A composition comprising an isolated nucleic acid molecule
having at least 80% sequence identity to any one of SEQ ID NOs:
763-1350 or 1565 or a complementary sequence thereof.
2. The composition of claim 1, wherein the isolated nucleic acid
molecule is a nucleic acid molecule having at least 90% sequence
identity to any one of SEQ ID NOs: 763-1350 or 1565 or a
complementary sequence thereof.
3. (canceled)
4. The composition of claim 1, wherein the isolated nucleic acid
molecule is a nucleic acid molecule comprising any one of SEQ ID
NOs: 763-1350 or 1565 or a complementary sequence thereof.
5. The composition of claim 1, wherein the isolated nucleic acid
molecule is a miRNA precursor molecule having the sequence of SEQ
ID NOs: 763-1350 or 1565, or a DNA molecule coding the miRNA.
6. The composition of claim 1, wherein the isolated nucleic acid
molecule is either single-stranded or at least partially
double-stranded.
7. (canceled)
8. The composition of claim 1, wherein the isolated nucleic acid
molecule is selected from RNA or DNA molecules.
9. The composition of claim 1, wherein the isolated nucleic acid
molecule is a cDNA that corresponds to any one of SEQ ID NOs:
763-1350 or 1565.
10. The composition of claim 1, wherein the isolated nucleic acid
molecule comprises at least one modified nucleotide analog.
11. The composition of claim 1, further comprising a carrier or
vehicle, wherein the isolated nucleic acid molecule is suitable as
a marker or modulator of a B cell malignancy, and wherein the
carrier or vehicle is a pharmaceutically acceptable carrier or
vehicle suitable for diagnostic or therapeutic applications.
12-14. (canceled)
15. The composition of claim 1, wherein the isolated nucleic acid
molecule is operably linked to a recombinant expression vector.
16. A cell comprising the recombinant expression vector of claim
15.
17. (canceled)
18. (canceled)
19. A method of identifying a B cell malignancy in a subject
comprising determining the level of expression of at least one
microRNA in a sample comprising a B cell taken from the subject
relative the level of expression of the at least one microRNA in a
control sample, wherein the at least one microRNA is selected from
the microRNAs listed in any one of Tables 4 and 7 to 35.
20. (canceled)
21. The method of claim 19, wherein the B cell malignancy is one of
at least two potential B cell malignancies, wherein at least two of
the potential B cell malignancies are derived from different B cell
stages.
22. (canceled)
23. The method of claim 19, wherein the B cell malignancy is
selected from chronic lymphocytic leukemia, follicular lymphoma,
Hodgkin's lymphoma, activated B-cell diffuse large B cell lymphoma
(DLBCL), germinal center-like DLBCL, and Burkitt lymphoma.
24-34. (canceled)
35. A kit comprising one or more sequences selected from a group
consisting of (a) at least one primer sequence that can detect any
one of SEQ ID NOs: 763-1350 or 1565, or a combination thereof; (b)
at least one isolated nucleic acid molecule having a sequence of
any one of SEQ ID NOs: 1351-1564; (c) the isolated nucleic acid
molecule of claim 1; and (d) any combination of (a)-(c).
36. (canceled)
37. The kit of claim 35, wherein (a) further comprises enzymes and
reagents that are adequate for performing an RT-PCR reaction.
38-41. (canceled)
42. The method of claim 19, further comprising determining whether
the B cell malignancy in the sample is Burkitt lymphoma, activated
B cell-like diffuse large B cell lymphoma (DLBCL), or germinal
center-like DLBCL, comprising determining the level of expression
of at least one microRNA in the sample relative to the level of
expression of the at least one microRNA in the control sample,
wherein the at least one microRNA is selected from the microRNAs
listed in Table 7, column "GCBvsBL"; Table 7, column "GCBvsABC";
Table 8, column "ABCvsBL"; Table 10, column "BL miRNA list"; Table
10, column "GCB miRNA list"; Table 11; Table 14, column "BL High";
Table 14, column "ABC High"; Table 14, column "GCB High"; Table 32;
or Table 35.
43. The method of claim 42, comprising determining the level of
expression of at least one microRNA selected from the microRNAs
listed in Table 10, column "BL miRNA list", and Table 14, column
"BL High"; determining the level of expression of at least one
microRNA selected from the microRNAs listed in Table 11 and Table
14, column "ABC High"; and determining the level of expression of
at least one microRNA selected from the microRNAs listed in Table
10, column "GCB miRNA list", and Table 14, column "GCB High".
44. The method of claim 43, wherein at least one microRNA is listed
in only one column selected from the columns labeled "GCBvsBL" or
"GCBvsABC" in Table 7 or "ABCvsBL" in Table 8.
45. The method of claim 42, wherein at least one microRNA is listed
in no more than two columns selected from the columns labeled
"GCBvsBL" or "GCBvsABC" in Table 7 or "ABCvsBL" in Table 8.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is related to and claims the benefit of
priority of U.S. Provisional Application Ser. No. 61/266,733, filed
Dec. 4, 2009, which is incorporated herein by, reference.
SEQUENCE LISTING
[0003] The sequence listing is filed with the application in
electronic format only and is incorporated by reference herein. The
sequence listing text file "WO_ASFILED_SequenceListing.txt" was
created on Dec. 3, 2010, and is 262,254 bytes in size.
BACKGROUND
[0004] Naive B cells migrate through the circulation to lymphoid
organs where they undergo the T cell-dependent germinal center
reaction. Adaptive immunity is acquired as specific
antigen-reactive germinal center B cells differentiate into the
major effector B cells of the adaptive immune system: memory cells
and plasma cells. See, e.g., FIG. 1A. Although the role of specific
transcription factors in mature B cell differentiation has been
examined (see Nutt et al. Nature. 1999; 401:556-562; Chang et al.
Proc Natl Acad Sci USA. 1996; 93:6947-6952; Turner et al. Cell.
1994; 77:297-306; Shaffer et al., Immunity. 2004; 21:81-93; and
Schebesta et al. Curr Opin Immunol. 2002; 14:216-223), mechanisms
regulating such transcription factors during mature B cell
differentiation are largely unknown.
[0005] Many malignancies derived from mature B cells are known and
are believed to constitute the majority of leukemias and lymphomas.
Such malignancies appear to reflect defined stages of normal B cell
differentiation. Diagnosis of leukemias and lymphomas can be
particularly difficult because of their shared lineage. These
cancers frequently display overlapping morphologies, genetic
abnormalities, and expression of surface markers, which can
complicate the diagnosis. However, the distinction of these tumors
is clinically important because there are important differences in
the treatments and expected response to treatment. Thus methods
that improve the accuracy of their diagnosis should provide to
improved outcomes for these patients.
[0006] MicroRNAs are commonly 18-22 nucleotide-long RNA molecules
that regulate expression of genes. There is an increasing
recognition of the role of microRNAs in oncogenesis,
lineage-selection, and immune cell function, including early B cell
differentiation. See Calin et al. N Engl J Med. 2005;
353:1793-1801; O'Donnell et al. Nature. 2005; 435:839-843; Chen et
al. Science. 2004; 303:83-86; Lim et al. Nature. 2005; 433:769-773;
Li et al. Cell. 2007; 129:147-161; Xiao et al., Nat Immunol. 2008;
9:405-414; Baltimore et al. Nat Immunol. 2008; 9:839-845; and
Ventura et al. Cell. 2008; 132:875-886. However, the full extent
and function of microRNA expression during mature B cell
differentiation and in B cell malignancies are not known.
[0007] Correct diagnosis of B cell malignancies is important from
both a clinical standpoint and from the standpoint of setting
appropriate patient expectations. A misdiagnosed B cell malignancy
may lead to an inappropriate therapy, which can unnecessarily
endanger the patient's life and/or be an ineffective treatment for
the B cell malignancy. As an example, the diagnostic distinction of
Burkitt lymphoma (BL) from diffuse large B cell lymphomas (DLBCLs)
can be difficult because of overlapping morphology, immunophenotype
and cytogenetics. Burkitt lymphoma tumors are molecularly distinct
from DLBCL, however. The difficulty and importance of obtaining the
correct diagnosis in BL was highlighted by the experience of a
multicenter clinical trial, CALGB trial#925119, in which nearly
half of the 100 patients with an assigned diagnosis of BL were
found to have another diagnosis upon further pathology review.
[0008] If diagnosed and treated appropriately, nearly 80% of
patients with BL can be cured with intensive (high dose)
chemotherapy regimens. Thus, a misclassification of BL as DLBCL can
result in a missed opportunity to cure the malignancy. On the other
hand, misclassification of DLBCL as BL leads to unnecessarily
morbidity from intensive chemotherapy regimens. Thus, methods that
improve the diagnosis of BL, and other B cell malignancies, can
provide better outcomes in patients.
[0009] DLBCLs can also be subclassified into two different B cell
malignancies, activated B-cell (ABC) DLBCL and germinal center B
cell like (GCB) DLBCL. There are at least two important clinical
applications for the molecular sub-grouping of DLBCL patients.
First, the prognostic information could inform the choices and
expectations of patients and their physicians. Second, the
important molecular differences in these subgroups form the basis
of testing different targeted therapies in these patients. The
possibility of a differential response to therapy among ABC and GCB
DLBCLs is supported by data that suggest that the benefit of
receiving a proteosome inhibitor, bortozemib, is predominantly
limited to those patients who have ABC DLBCL. However, the clinical
distinction of the subgroups of DLBCL using immunohistochemistry is
difficult with current methods distinguishing GCB DLBCL from
non-GCB DLBCL with limited success.
[0010] Mature B cell differentiation is important for the
development of adaptive immunity. The process is also of interest
because B cell malignancies are common and retain a number of
features derived from their normal counterpart B cell subsets.
Unlike other maturation pathways in the hematopoietic and other
cell lineages, successive stages of mature B cells do not simply
signify progressive differentiation away from the stem cell stage.
Rather, each stage represents a specialized state with specific
functions. Thus, germinal center (GC) cells interact with CD4 T
cells and dendritic cells and undergo somatic hypermutation and
Ig-heavy chain class-switching. On the other hand, plasma cells
secrete immunoglobulin, while memory cells are primed to
proliferate and differentiate into plasma cells upon repeat contact
with antigen. The specialized functions demand a finely tuned
program of gene regulation.
[0011] MicroRNAs represent a novel class of biomarkers that provide
new opportunities for clinical translation. First, intact microRNAs
can be isolated from tissues preserved using standard methods, such
as formalin fixed, paraffin embedded (FFPE) tissue. Thus,
microRNA-based biomarkers could be easy to translate to clinical
use. Second, microRNAs can be readily assayed using real-time PCR
and other methods available in conventional pathology.
SUMMARY
[0012] In an aspect the disclosure provides an isolated nucleic
acid molecule having at least 80% sequence identity to any one of
SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof.
Embodiments provide for sequence identity of at least 90% or
95%.
[0013] In an aspect the disclosure provides an isolated nucleic
acid molecule comprises any one of SEQ ID NOs: 763-1350 or 1565 or
a complementary sequence thereof. Embodiments provide for isolated
nucleic acid molecules comprising a primary miRNA, a precursor
miRNA, a mature miRNA, or a DNA molecule coding therefore.
Embodiments further provide for a cDNA molecule comprising sequence
that corresponds to a miRNA sequence of any one of SEQ ID NOs:
763-1350 or 1565.
[0014] Aspects of the disclosure provide compositions,
pharmaceutical compositions, vectors, host cells, and DNA libraries
comprising at least one nucleic acid molecule described herein.
[0015] In an aspect the disclosure provides a method of determining
the B cell stage of a B cell malignancy in a subject comprising
determining the level of expression of at least one microRNA in a
sample comprising a B cell taken from the subject relative the
level of expression of the at least one microRNA in a control
sample, wherein the at least one microRNA is selected from the
microRNAs listed in Table 4.
[0016] In an aspect the disclosure provides a method of identifying
a B cell malignancy in a subject comprising determining the level
of expression of at least one microRNA in a sample comprising a B
cell taken from the subject relative the level of expression of the
at least one microRNA in a control sample, wherein the at least one
microRNA is selected from the microRNAs listed in Table 4.
[0017] In an aspect the disclosure provides a method of diagnosing
a B cell malignancy in a subject comprising determining the level
of expression of at least one microRNA in a sample comprising a B
cell taken from the subject relative the level of expression of the
at least one microRNA in a control sample, wherein the at least one
microRNA is selected from the microRNAs listed in Table 4.
[0018] In an aspect the disclosure provides a method of identifying
a B cell malignancy in a subject comprising determining the level
of expression of at least one microRNA in a sample comprising a B
cell taken from the subject relative the level of expression of the
at least one microRNA in a control sample, wherein the at least one
microRNA is selected from the microRNAs listed in any one of Tables
7 to 35.
[0019] In an aspect the disclosure provides a method of diagnosing
a B cell malignancy in a subject comprising determining the level
of expression of at least one microRNA in a sample comprising a B
cell taken from the subject relative to the level of expression of
the at least one microRNA in a control sample, wherein the at least
one microRNA is selected from the microRNAs listed in any one of
Tables 7 to 35.
[0020] Embodiments of these aspects provide for identification or
diagnosis of a B cell malignancy selected from chronic lymphocytic
leukemia, follicular lymphoma, Hodgkin's lymphoma, activated B-cell
diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL,
and Burkitt lymphoma.
[0021] In an aspect the disclosure provides a method of determining
whether a B cell malignancy in a sample is Burkitt lymphoma,
activated B cell-like diffuse large B cell lymphoma (DLBCL), or
germinal center-like DLBCL, comprising determining the level of
expression of at least one microRNA in the sample relative to the
level of expression of the at least one microRNA in a control
sample, wherein the at least one microRNA is selected from the
microRNAs listed in Table 10, column "BL miRNA list"; Table 14,
column "BL High"; Table 11; Table 14, column "ABC High"; Table 10,
column "GCB miRNA list"; Table 14, column "GCB High"; Table 32, or
Table 35.
[0022] In an aspect the disclosure provides a method of determining
whether a B cell malignancy in a sample is Burkitt lymphoma,
activated B cell-like diffuse large B cell lymphoma (DLBCL), or
germinal center-like DLBCL, comprising determining the level of
expression of at least one microRNA in the sample relative to the
level of expression of the at least one microRNA in a control
sample, wherein the at least one microRNA is selected from the
microRNAs listed in at least one column labeled "GCBvsBL" or
"GCBvsABC" in Table 7 or "ABCvsBL" in Table 8.
[0023] In an aspect the disclosure provides a method of determining
whether a B cell malignancy in a sample is activated B cell-like
diffuse large B cell lymphoma (DLBCL), or germinal center-like
DLBCL, comprising determining the level of expression of at least
one microRNA in the sample relative to the level of expression of
the at least one microRNA in a control sample, wherein the at least
one microRNA is selected from the microRNAs listed in Table 35.
[0024] In an aspect the disclosure provides a microarray comprising
miRNA-specific probe oligonucleotides wherein at least one
miRNA-specific probe oligonucleotide is specific for a sequence of
SEQ ID NOs: 763-1350 or 1565, or any combination thereof.
[0025] In an aspect the disclosure provides a kit comprising at
least one primer sequence that can detect any one of SEQ ID NOs:
763-1350 or 1565, or a combination thereof.
[0026] In an aspect the disclosure provides a kit comprising at
least one isolated nucleic acid molecule having a sequence of any
one of SEQ ID NOs: 1351-1564.
[0027] In an aspect, the disclosure provides a method of
identifying a B cell malignancy comprising determining the level of
expression of at least one microRNA selected from the microRNAs
listed in Table 4. In certain embodiments, a method comprises
determining the level of expression of at least one microRNA
selected from the microRNAs listed in Tables 7-35.
[0028] Other aspects and embodiments will be apparent to one of
skill in the art in light of the following detailed
description.
DESCRIPTION OF THE FIGURES
[0029] FIG. 1 shows that mature B cell subsets demonstrate distinct
microRNA profiles. FIG. 1A is a diagram showing the overall schema
of mature B cell differentiation. FIG. 1B shows selection of B cell
subsets using flow cytometry. FIG. 1C shows the distinction between
naive and memory B cells based on IgD and CD27 expression using
flow cytometry. FIG. 1D shows relative expression of microRNA in
the naive to germinal center B cell transition. FIG. 1E shows
relative expression of mRNA in the naive to germinal center B cell
transition. FIG. 1F shows relative expression of microRNA in the
germinal center B cell to plasma cell transition. FIG. 1G shows
relative expression of mRNA in the germinal center B cell to plasma
cell transition. FIG. 1H shows relative expression of microRNA in
the germinal center B cell to memory B cell transition. FIG. 1I
shows relative expression of mRNA in the germinal center B cell to
memory B cell transition. In FIGS. 1D, 1F, and 1H, miRNAs that
were, on average, at least 2-fold differentially expressed at a
false discovery rate of less than 5% are shown according to the
color scale. In Figures and 1E, 1G, and 1I, mRNAs that were, on
average, at least 2-fold differentially expressed at a false
discovery rate of less than 1% are shown according to the color
scale. FIG. 1J shows that expression of certain microRNA processing
genes, DGCR8, DICER1, EIF2C2, DROSHA, and XP05 is unchanged among
the B cell subsets (P>0.1 in all cases).
[0030] FIG. 2 shows experimental validation of the interaction of
miR-223, which is expressed highly in naive and memory B cells
compared to germinal center B cells, and targets the transcription
factor LM02. FIG. 2A shows base-pairing of the 3'UTR of the LM02
gene with nucleotides 1-8 of miR-223. This 8-mer is highly
conserved across a number of species and serves as a potential
binding site for miR-223. FIG. 2B shows the effects of
over-expression of miR-223 in germinal center lymphoma-derived BJAB
cells in 3 separate experiments. FIG. 2C shows the relative LM02
protein expression from a representative experiment (from 3
replicates) transfecting a scrambled control versus a precursor for
miR-223 in BJAB cells. FIG. 2D shows average expression of LM02
relative to Actin over three Western blots of BJAB cells
transfected with a scrambled control versus a precursor for
miR-223. FIG. 2E shows luciferase activity in BJAB cells
transfected with a vector comprising either a luciferase gene
coupled to the 3 'UTR of the LM02 gene or a luciferase gene coupled
to the 3 'UTR of the LM02 gene with the miR-223 binding site
mutated, and cotransfected with miR-223.
[0031] FIG. 3 shows experimental validation of the interaction of
miR-9 and miR-30, which are expressed highly in germinal center B
cells compared to plasma cells and target the transcription factor
PRDM1. FIG. 3A shows base-pairing of the 3'UTR of the PRDM1 gene
with the 5' seed region of miR-9 and the miR-30 family. FIG. 3B
shows the effects of over-expressing miR-9 and 2 members of the
miR-30 family, miR-30b and miR-30d, in plasma cell myeloma-derived
U266 cells in 3 separate experiments. FIG. 3C shows the relative
PRDM1 protein expression from a representative experiment (from 3
replicates) transfecting a scrambled control versus a precursor for
miR-9, miR-30b, or miR-30d in U266 cells. FIG. 3D shows the average
expression of PRDM1 relative to Actin over three Western blots of
U266 cells transfected with a scrambled control versus a precursor
for miR-9, miR-30b, or miR-30d. (P<0.05 for miR-30b and miR-30d,
P=0.08 for miR-9.) FIG. 3E shows repression of luciferase activity
from the PRDM1 3'UTR construct by overexpression of miR-9, miR-30b,
and miR-30d wild-type and mutant sequences.
[0032] FIG. 4 shows that expression of microRNAs in normal B cells
is conserved in certain B cell malignancies. FIG. 4A shows lineage
prediction of both IgV mutated and unmutated chronic lymphocytic
leukemia, germinal center B cell derived DLBCL, and Burkitt
lymphoma based on differential expression of microRNAs in normal
naive B cells and germinal center B cells (microRNAs depicted in
FIG. 1D). FIG. 4B shows miRNAs that were found to be differentially
expressed (P<0.05) in malignant cells and normal cells as
discussed in Example 5. FIG. 4C shows cloning frequency of miRNAs
in unselected mature B cells (N=3) and certain B cell malignancies
(N=42) from a previously published study ("sequencing data"), as
discussed in Example 5. FIG. 4D shows differentially expressed
miRNAs that distinguish Burkitt lymphoma, activated B cell-like
(ABC) diffuse large B cell lymphoma (DLBCL), germinal center-like
DLBCL (GCB DLBCL), and chronic lymphocytic leukemia. Predictor
miRNAs from each pair-wise comparison that distinguish each entity
are shown in the boxes.
[0033] FIG. 5 shows the distribution of miRNAs present in B-cell
subsets.
[0034] FIG. 6 shows that certain microRNA targets are expressed at
lower levels. FIG. 6A shows density plots of the expression
frequency of predicted mRNA targets of miRNAs expressed highly in
germinal center B cells compared to naive cells. FIG. 6B shows
density plots of the expression frequency of predicted mRNA targets
of miRNAs expressed highly in germinal center B cells compared to
plasma cells. FIG. 6C shows density plots of the expression
frequency of predicted mRNA targets of miRNAs expressed highly in
the germinal center B cells compared to memory B cells.
[0035] FIG. 7 shows that differentially expressed transcription
factors are frequently microRNA targets. In FIG. 7A, the left panel
indicates the proportion of transcription factors that are
differentially expressed in the naive to germinal center B-cell
transition that are also predicted targets of differentially
expressed miRNAs. The right panel indicates the proportion of
transcription factors that are not differentially expressed and
also are predicted targets of differentially expressed miRNA in
that stage-transition. The p-value indicates the results of a
chi-squared test for the enrichment of predicted miRNA targets
among the differentially expressed transcription factors. FIG. 7B
shows a similar analysis of the germinal center to plasma cell
transition. FIG. 7C shows a similar analysis of the germinal center
to memory cell transition.
[0036] FIG. 8 shows that Mybl transcript levels decrease with
miR-223 overexpression. FIG. 8A shows base-pairing of the 3'UTR of
the MYBL1 gene with nucleotides 2-8 of miR-223. This 7-mer is
highly conserved across a number of species and serves as a
potential binding site for miR-223. FIG. 8B shows the effects of
over-expression of miR-223 in germinal center lymphoma-derived BJAB
cells in 3 separate experiments.
[0037] FIG. 9 shows the specificity of real-time PCR probes for
members of the miR-30 family.
[0038] FIG. 10 shows the results of leave one out cross validation
applied to the predictors for Burkitt lymphoma, chronic lymphocytic
leukemia, activated B-cell diffuse large B-cell lymphoma, and
germinal center B-cell DLBCL.
[0039] FIG. 11 shows differentially expressed miRNAs that
distinguish activated B cell-like diffuse large B cell lymphoma
(DLBCL), germinal center-like DLBCL (GCB DLBCL), Burkitt lymphoma,
chronic lymphocytic leukemia, follicular lymphoma, and Hodgkin's
lymphoma. Predictor miRNAs from each pair-wise comparison that
distinguish each entity are shown in the boxes.
DETAILED DESCRIPTION
[0040] All patent and non-patent literature references that are
cited herein are incorporated herein by reference in their
entirety.
[0041] In a general sense, the disclosure relates to nucleic acid
sequences, such as microRNAs (miRNA), as well as to the
identification and analysis of microRNA expression levels and/or
patterns in B cells. Through concomitant microRNA and mRNA
profiling, the inventors have identified regulatory roles for
microRNAs at each stage in mature B cell differentiation. This
provides methods identifying microRNA-mediated regulation of
oncogenes and key transcription factors in B cell differentiation.
This work establishes the landscape of normal microRNA expression
in mature B cells and its role in regulating normal B cell
differentiation. Further, our work demonstrates that in contrast to
the described down-regulation in other malignancies, stage-specific
microRNAs are retained in B cell malignancies. The lineage of
common B cell malignancies can be predicted based upon miRNA
profiles of normal B cells, pointing to a role for microRNAs in the
maintenance of mature B cell phenotypes in normal and malignant B
cells.
[0042] In an aspect, the disclosure relates to an isolated nucleic
acid molecule comprising: (a) a nucleotide sequence as shown in
Table 32; (b) a nucleotide sequence which is the complement of (a),
(c) a nucleotide sequence comprising a sequence identity of at
least 80%, (e.g., 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91,
92, 93, 94, 95, 96, 97, 98, or at least 99%), to a sequence of (a)
or (b) and/or (d) a nucleotide sequence which hybridizes under
stringent conditions to a sequence of (a), (b) and/or (c). In some
embodiments, the identity of sequence (c) to a sequence of (a) or
(b) is at least 90%. In other embodiments, the identity of sequence
(c) to a sequence of (a) or (b) at least 95%. The percent identity
can be calculated by any routine method used by one of skill in the
art such as, for example, the methods described herein.
[0043] In embodiments, the isolated nucleic acid molecule relates
to a miRNA molecule and analogs thereof, a miRNA precursor
molecule, or a primary miRNA molecule, as well as to DNA molecules
encoding miRNA, miRNA precursor, or primary miRNA molecules.
Accordingly, in such embodiments, the isolated nucleic acid
molecule can function as a miRNA molecule under suitable
conditions. Suitable conditions include, but are not limited to,
various buffer systems that approximate physiologically relevant
ionic concentrations and pHs, as well as physiological
conditions.
[0044] In some embodiments, the nucleic acid molecule comprises a
sequence that hybridizes to a nucleotide sequence as shown in Table
32, a complementary sequence thereof or a nucleic acid molecule
having at least 80% sequence identity under stringent hybridization
conditions. The basic parameters affecting the choice of
hybridization conditions and guidance for devising suitable
conditions are set forth by Sambrook, et al. (See, 1989, Molecular
Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press,
Cold Spring Harbor, N.Y.; and Current Protocols in Molecular
Biology, 1995, Ausubel et al., eds., John Wiley & Sons, Inc.),
and can be readily determined by those of ordinary skill in the art
based on, for example, the length and/or base composition of the
DNA. Generally, stringent salt concentration will ordinarily be
less than about 750 mM NaCl and 75 mM trisodium citrate, or less
than about 500 mM NaCl and 50 mM trisodium citrate, or even less
than about 250 mM NaCl and 25 mM trisodium citrate. High stringency
hybridization conditions can be obtained by adding an amount of
organic solvent (e.g., at least about 35% to about 50% formamide).
Stringent temperature conditions will ordinarily include
temperatures of at least about 30.degree. C., (e.g., at least about
37.degree. C., 42.degree. C., 45.degree. C., 50.degree. C., or
55.degree. C.). Varying additional parameters, such as
hybridization time, the concentration of detergent (e.g., 0.1-1.0%
sodium dodecyl sulfate (SDS)), and the inclusion or exclusion of
carrier DNA (e.g., about 100-200 .mu.g/ml denatured salmon sperm
DNA (ssDNA)), are well known to those skilled in the art. Stringent
hybridization conditions are known in the art and include
non-limiting examples such as, washing for 1 hr in 300 mM NaCl, 30
mM trisodium citrate and 0.1% SDS at 45-50.degree. C.; washing for
1 h in 300 mM NaCl, 30 mM trisodium citrate and 0.1% SDS at
45-50.degree. C.; in 500 mM NaCl, 50 mM trisodium citrate, 1% SDS,
35% formamide, and 100 .mu.g/ml denatured salmon sperm DNA (ssDNA)
at 37.degree. C.; or in 250 mM NaCl, 25 mM trisodium citrate, 1%
SDS, 50% formamide, and 200 .mu.g/ml ssDNA at 42.degree. C. Useful
variations on these conditions will be readily apparent to those
skilled in the art.
[0045] The isolated nucleic acid molecules provided herein suitably
have a length of from about 18 to about 100 nucleotides. In
embodiments wherein the isolated nucleic acid molecules are miRNAs,
the lengths of the miRNAs are suitably in an expected range for the
particular type of miRNA molecule. For example, mature miRNAs are
typically from about 15 to about 28 nucleotides in length, and
suitably have a length of about 19 to about 24 nucleotides (e.g.,
19, 20, 21, 22, 23, or 24 nucleotides). Precursor miRNAs typically
comprise the mature miRNA sequence and contain a stem-loop
structure, suitably of length of about 50 to about 90 nucleotides
(e.g., 50, 55, 60, 65, 70, 75, 80, 85, or 90 nucleotides). Primary
miRNAs (e.g., a primary transcript comprising a precursor miRNA)
can suitably have a length of greater than 100 nucleotides.
[0046] The nucleic acid molecules can be provided in either a
single-stranded or double-stranded form. Typically, a miRNA as such
is identified as a single-stranded molecule, while the precursor
miRNA is typically at least partially self-complementary and
capable of forming double-stranded portions, e.g. stem- and
loop-structures. DNA molecules encoding the miRNA and miRNA
precursor molecules (e.g., expression vectors, cloning vectors, and
the like) are typically double-stranded. The individual nucleic
acids that comprise the isolated nucleic acid molecules can be
selected from RNA, DNA, or nucleic acid analog molecules, such as
chemically modified sugar (e.g., 2'-modified (2'-F, 2'-OMe, etc.)
or backbone (e.g., phosphorothioates), or cap (e.g., 5'- and/or
3'-abasic groups) moieties of ribonucleotides or
deoxyribonucleotides. Other nucleic acid analogs, such as peptide
nucleic acids (PNA) or locked nucleic acids (LNA), are also
suitable in various embodiments described herein. In some
embodiments the nucleic acid molecules can comprise any combination
of nucleic acid analog(s).
[0047] While many of the nucleic acid molecules in the Tables are
identified as RNA sequences, e.g., miRNAs, the disclosure of those
sequences should be understood to encompass the corresponding DNA
(e.g., cDNA) sequences, wherein the uracil (U) nucleotides of the
disclosed RNAs are substituted by thymidine (T) nucleotides in the
corresponding DNA. One of skill in the art is able to generate such
DNA sequences (e.g., cDNA) through routine microbiological
techniques known in the art such as, for example, reverse
transcription using methods that incorporate the reverse
transcriptase enzyme.
[0048] In another aspect, the disclosure provides a recombinant
expression vector comprising a recombinant nucleic acid sequence
operatively linked to an expression control sequence, wherein
expression of the recombinant nucleic acid sequence provides a
miRNA sequence, a precursor miRNA sequence, or a primary miRNA
sequence as described herein. The resulting sequence (e.g., primary
or precursor miRNAs) can optionally be further processed to provide
the miRNA sequence. In embodiments, the recombinant expression
vector comprises at least one sequence in Table 32. Any suitable
expression vector can be used such as, for example, a DNA vector
(e.g., viral vector, plasmid, etc.). In some embodiments the
expression vector is selected for expression in a eukaryotic cell
such as, for example, a mammalian cell. One of skill in the art
will be able to select an appropriate vector based on the
particular application and/or expression system to be employed.
[0049] Thus, embodiments provide nucleic acid constructs in the
form of plasmids, vectors, transcription or expression cassettes
which comprise at least one nucleotide sequence encoding a miRNA
described herein, or fragments thereof, and a suitable promoter
region. Suitable vectors can be chosen or constructed, which
contain appropriate regulatory sequences, such as promoter
sequences, terminator sequences, polyadenylation sequences,
enhancer sequences, marker genes and other sequences as desired.
Vectors can be plasmids, phage (e.g. phage, or phagemid) or viral
(e.g. lentivirus, adenovirus, AAV) or any other appropriate vector.
For further details see, for example, Molecular Cloning: a
Laboratory Manual: 2nd edition, Sambrook et al., 1989, Cold Spring
Harbor Laboratory Press.
[0050] Relatedness of Nucleic Acid Molecules/Sequences
[0051] The term "identity" refers to a relationship between the
sequences of two or more two or more nucleic acid molecules, as
determined by comparing the sequences. In the art, "identity" also
means the degree of sequence relatedness between amino acid or
nucleic acid molecule sequences, as the case may be, as determined
by the match between strings of nucleotide or amino acid sequences.
"Identity" measures the percent of identical matches between two or
more sequences with gap alignments addressed by a particular
mathematical model or computer programs (i.e., "algorithms").
[0052] Identity of related nucleic acid molecules can be readily
calculated by known methods, including but not limited to those
described in Computational Molecular Biology, Lesk, A. M., ed.,
Oxford University Press, New York, 1988; Biocomputing: Informatics
and Genome Projects, Smith, D. W., ed., Academic Press, New York,
19933; Computer Analysis of Sequence Data, Part 1, Griffin, A. M.,
and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence
Analysis in Molecular Biology, von Heinje, G., Academic Press,
1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J.,
eds., M. Stockton Press, New York, 1991; and Carillo, H., and
Lipman, D., SIAM J. Applied Math., 48:1073 (1988).
[0053] Non-limiting methods for determining identity are designed
to give the largest match between the sequences tested. Methods to
determine identity are codified in publicly available computer
programs. Preferred computer program methods to determine identity
between two sequences include, but are not limited to, the GCG
program package, including GAP (Devereux, et al., Nucleic Acids
Research 12:387 [1984]; Genetics Computer Group, University of
Wisconsin, Madison, Wis.), BLASTN, and FASTA (Atschul et al., J.
Molec. Biol. 215:403-410 [1990]). The BLAST X program is publicly
available from the National Center for Biotechnology Information
(NCBI) and other sources (BLAST Manual, Altschul] et al., NCB NLM
NIH Bethesda, Md. 20894; Altschul et al., J. Mol. Biol. 215:403-410
[1990]). The well known Smith Waterman algorithm may also be used
to determine identity.
[0054] Exemplary parameters for nucleic acid molecule sequence
comparison include the following:
[0055] Algorithm: Needleman and Wunsch, J. Mol Biol. 48:443-453
(1970)
[0056] Comparison matrix: matches=+10, mismatch=0
[0057] Gap Penalty: 50
[0058] Gap Length Penalty: 3
[0059] The GAP program is also useful with the above parameters.
The aforementioned parameters are the default parameters for
nucleic acid molecule comparisons.
[0060] Other exemplary algorithms, gap opening penalties, gap
extension penalties, comparison matrices, thresholds of similarity,
etc. can be used by those of skill in the art, including those set
forth in the Program Manual, Wisconsin Package, Version 9,
September 1997. The particular choices to be made will depend on
the specific comparison to be made, such as DNA to DNA or RNA to
DNA; and additionally, whether the comparison is between given
pairs of sequences (in which case GAP or BestFit are generally
preferred) or between one sequence and a large database of
sequences (in which case FASTA or BLASTA are preferred).
[0061] In an aspect, the disclosure provides a vector comprising
the isolated polynucleotide as described herein such as, for
example one or more of SEQ ID NOs 773-1046 or 1450-1542. In
embodiments, the vector can be any type of vector that finds use as
a vehicle to transfer foreign genetic material into a cell.
Non-limiting examples of vectors include plasmids, viral vectors
(e.g., derived from lentivirus, adenovirus, adeno-associated virus
(AAV), retrovirus, etc.), bacteriophage, cosmids, and artificial
chromosomes. In embodiments, the vector can be an expression (or
expression constructs) for driving expression of the polynucleotide
in a target cell. Vectors and methods for inserting them into a
target cell are known in the art [See, e.g., Sambrook et al.,
1989].
[0062] In an aspect, the disclosure provides recombinant cells that
comprise the vectors and/or polynucleotides described herein. The
cells can be any cell suitable as a host for recombinant nucleic
acid molecules, and selected based on well known techniques.
Techniques for generating and maintaining recombinant cells are
known in the art, such as those described in Sambrook et al.,
1989.
[0063] The term "B cell malignancy," as used herein, refers to a
malignancy derived from any stage of B cell, including, but not
limited to, naive cells, germinal center cells, memory B cells, and
plasma cells. Examples of B cell malignancies include, but are not
limited to, mantle cell lymphoma, follicular lymphoma, Hodgkin's
lymphoma, Burkitt lymphoma, germinal center B-cell like diffuse
large B cell lymphoma (DLBCL), chronic lymphocytic leukemia, small
lymphocytic lymphoma, lymphoplasmacytic lymphoma, multiple myeloma,
and activated B-cell like DLBCL.
[0064] In an aspect, the disclosure provides a method of
distinguishing B cell malignancies on the basis of the B-cell
origin. In certain embodiments, methods of diagnosing B cell
malignancies on the basis of the B-cell origin are provided. In
certain such embodiments, a B cell malignancy is determined to be
derived from a particular B-cell stage. The B-cell origin of a B
cell malignancy may be determined, in certain embodiments, by
detecting one or more microRNAs that can be used to distinguish
B-cell stages. Certain exemplary B-cell stages include, but are not
limited to, naive cells, germinal center cells, memory B cells, and
plasma cells. Certain exemplary microRNAs that can be used to
distinguish B-cell stages are shown in Table 4. In various
embodiments, the method comprises detecting at least one, at least
two, at least five, at least 10, at least 20, at least 30, at least
50, at least 75, or at least 100 microRNAs.
[0065] In certain embodiments, a panel of microRNAs is selected
that will allow determination of the B cell stage from which a B
cell malignancy is derived. For example, in certain embodiments,
two or more microRNAs from Table 4 are selected such that detection
of the levels of those microRNAs in a B cell malignancy will
indicate whether the B cell malignancy is derived from naive,
germinal center, plasma, or memory B cells. In various embodiments,
the panel of microRNAs comprises at least one, at least two, at
least five, at least 10, at least 20, at least 30, at least 50, at
least 75, or at least 100 microRNAs from Table 4. One skilled in
the art can select a suitable panel of microRNAs, including one or
more microRNAs from Table 4, according to the intended use of the
panel.
[0066] As described throughout the disclosure, the methods herein
can include detecting one or a plurality of miRNAs. When the term
"at least" is used in association with a number (e.g., "at least
20") that term will be understood to include 20 as well as
optionally any integer after 20 and up to and including the total
number of microRNAs disclosed herein.
[0067] In some embodiments, a B cell malignancy derived from naive
cells is mantle cell lymphoma. In other embodiments, a B cell
malignancy derived from germinal center cells includes, but is not
limited to, follicular lymphoma, Hodgkin's lymphoma, Burkitt
lymphoma, or germinal center B-cell like diffuse large B cell
lymphoma (DLBCL). In other embodiments, a B cell malignancy derived
from memory B cells includes, but is not limited to, chronic
lymphocytic leukemia or small lymphocytic lymphoma. In some
embodiments, a B cell malignancy derived from plasma cells
includes, but is not limited to, multiple myeloma or activated
B-cell DLBCL.
[0068] Certain B cell malignancies can be difficult to distinguish
using current methodologies. In extreme cases, almost any B cell
malignancy can be confused with another. As illustrative examples,
Burkitt lymphoma and DLBCLs are often confused. Similarly, mantle
cell lymphoma and small lymphocytic lymphoma can also be confused.
Burkitt lymphoma and germinal center DLBCL are both derived from
germinal center cells, while activated B-cell DLBCL is derived from
plasma cells. Thus, if a B cell malignancy appears to be Burkitt
lymphoma or a DLBCL, in certain embodiments, microRNA analysis can
be used to narrow down the B cell malignancy to either a germinal
center cell-derived B cell malignancy or a plasma cell-derived B
cell malignancy. If the B cell malignancy is plasma-cell derived,
then it may be activated B-cell DLBCL.
[0069] Thus, in certain embodiments, when the identity of a
particular B cell malignancy has been narrowed down to two or more
possible B cell malignancies, and at least two of those B cell
malignancies are derived from different B cell stages, microRNAs
that distinguish certain B cell stages can be used to further
narrow down the identity of the B cell malignancy. In certain
embodiments, microRNAs that distinguish certain B cell stages can
be used to identify the B cell malignancy. One or more such
microRNAs can be selected, in certain embodiments, from the
microRNAs in Table 4. One skilled in the art can select a suitable
set of microRNAs, including at least one microRNA from Table 4, for
distinguishing particular B cell stages.
[0070] In an aspect, the disclosure provides a method of
identifying a B cell malignancy comprising detecting one or a
plurality of microRNAs. In certain embodiments, the method can
provide a diagnosis of a B cell malignancy. In certain embodiments,
one or more microRNAs that are characteristic of a particular B
cell malignancy are used to identify the B cell malignancy. In
certain embodiments, the identity of the B cell malignancy is first
narrowed down to a list of two or more particular B cell
malignancies using, for example, tumor morphology and/or
immunohistochemistry and/or microRNA detection, e.g., to determine
the B cell stage from which the tumor is derived. Certain exemplary
microRNAs that can be used to identify B cell malignancies are
shown in the Tables (e.g., Tables 7-35).
[0071] In certain embodiments, methods of identifying B cell
malignancies comprise detecting one or more microRNAs from one or
more of Tables 7 to 15, and Appendix B, Tables 16 to 30. That is,
in certain embodiments, a panel of microRNAs is selected that will
identify a B cell malignancy as being one of a particular selection
of B cell malignancies. As a non-limiting example, a panel of
microRNAs can be designed to identify a B cell malignancy as one of
Burkitt lymphoma, ABC DLBCL, or GCB DLBCL. In certain such
embodiments, the panel of microRNAs comprises at least one microRNA
from Table 10, column "BL miRNA list" and/or Table 14, column "BL
High"; at least one microRNA from Table 11 and/or Table 14, column
"ABC High"; and at least one microRNA from Table 10, column "GCB
miRNA list" and/or Table 14, column "GCB High".
[0072] When other methods indicate a particular identity for a B
cell malignancy, in certain embodiments, microRNAs can be used to
confirm that identification. Thus, for example, if a B cell
malignancy is believed to be a Burkitt lymphoma, that
identification can be confirmed by determining the expression level
of one or more microRNAs listed in Table 10, column "BL miRNA list"
and/or Table 14, column "BL High". Similarly, if a B cell
malignancy is believed to be ABC DLBCL, that identification can be
confirmed by determining the expression level of one or more
microRNAs listed in Table 11 and/or Table 14, column "ABC High". If
a B cell malignancy is believed to be GCB DLBCL, that
identification can be confirmed by determining the expression level
of one or more microRNAs listed in Table 10, column "GCB miRNA
list" and/or Table 14, column "GCB High". If a B cell malignancy is
believed to be chronic lymphocytic leukemia, that identification
can be confirmed by determining the expression level of one or more
microRNAs listed in Table 12 and/or Table 15, column "CLL High". If
a B cell malignancy is believed to be Hodgkin's lymphoma, that
identification can be confirmed by determining the expression level
of one or more microRNAs listed in Table 13 and/or Table 15, column
"HL High". If a B cell malignancy is believed to be follicular
lymphoma, that identification can be confirmed by determining the
expression level of one or more microRNAs listed in Table 10,
column "FL miRNA list" and/or Table 15, column "FL High".
[0073] In certain embodiments, when the identity of a B cell
malignancy has been narrowed down to two B cell malignancies
selected from Burkitt lymphoma, GCB DLBCL, ABC DLBCL, chronic
lymphocytic leukemia, follicular lymphoma, and Hodgkin's lymphoma,
the identity of the B cell malignancy can be determined by
detecting one or more microRNAs from Tables 16 to 30.
[0074] As discussed in the Examples, the miRNAs described herein as
differentially expressed in a B cell malignancy have been
identified with high confidence, and thus, identification of one
miRNA is adequate to perform the methods of identification and
diagnosis disclosed herein. Accordingly, in various embodiments,
the methods can comprise detecting at least one, at least two, at
least five, at least 10, at least 20, at least 30, or at least 50
microRNAs in order to narrow down the identity of, or identify, a B
cell malignancy.
[0075] As noted above, the treatment regimens and prognoses for the
various B cell malignancies can differ significantly. Thus,
determining the correct identity and/or origin of a B cell
malignancy can be important for selecting an effective therapy
and/or setting appropriate patient expectations.
[0076] B cell malignancy samples may be obtained and prepared using
methods known in the art. One skilled in the art can select an
appropriate method of obtaining a B cell malignancy sample
according to various parameters, such as the age, size, medical
history, and/or identity of the patient. One skilled in the art can
select an appropriate method of preparing a B cell malignancy
sample for analysis according to the B cell malignancy sample
source, size, quality, and/or intended use. For example, in certain
embodiments, a B cell malignancy sample is prepared in a manner
that preserves microRNAs in the sample as much as practicable under
the circumstances.
[0077] MicroRNAs can be detected using any method known in the art.
Exemplary methods of detecting microRNAs include, but are not
limited to, hybridization-based methods and amplification-based
methods. Certain exemplary detection methods include, but are not
limited to, arrays (including microarrays and bead-based arrays),
in situ hybridization, Northern blotting, TaqMan probes, RT-PCR,
real-time PCR, and direct sequencing. One skilled in the art can
select a suitable detection method according to the sample source,
size, quality, and/or particular application.
[0078] In certain embodiments, real-time PCR is employed to
determine the expression level of a microRNA. In some embodiments a
miRNA is considered present in a subpopulation if the cycling time
(CT) is less than 36 in all three biological replicates, and a CT
greater than 36 is undetected.
[0079] In certain embodiments, the expression level of a microRNA
in a sample is determined relative to a control sample. A control
sample may be selected, in various embodiments, because it is
expected to have either high or low expression of the microRNA.
[0080] In certain embodiments, the expression level of a microRNA
may be normalized to the expression level of a polynucleotide that
is expected to be expressed at similar levels in several different
cell types and/or at constant levels in the cell type being
analyzed.
[0081] In certain embodiments, an identified miRNA from Tables 7-35
is used to distinguish one of the six exemplified B cell
malignancies from the other malignancies. A "high" and a "low" in
Tables 10 to 13 refer to at least a 2-fold difference in the
expression of the identified miRNA when one lymphoma is compared to
other lymphomas and benign lymph nodes.
[0082] In embodiments, mRNA levels can be profiled by using a
microarray. In some embodiments, array elements with median signal
intensities of less than 7 log 2 units across samples are removed
from analysis. In embodiments, a gene is considered for further
analysis if it is on-average 2-fold or higher differentially
expressed in a binary comparison of B cell subsets and expressed in
at least one of the two B cell subsets being compared.
[0083] In an embodiment, the level of at least one miRNA is
measured by reverse transcribing RNA from a test sample obtained
from a subject to provide a set of target oligodeoxynucleotides,
hybridizing the target oligodeoxynucleotides to one or more
miRNA-specific probe oligonucleotides (e.g., a microarray that
comprises miRNA-specific probe oligonucleotides) to provide a
hybridization profile for the test sample, and comparing the test
sample hybridization profile to a hybridization profile generated
from a control sample. An alteration in the signal of at least one
miRNA in the test sample relative to the control sample is
indicative of the subject either having, or being at risk for
developing, a B-cell malignancy. In an embodiment, the signal of at
least one miRNA is upregulated, relative to the signal generated
from the control sample. In another embodiment, the signal of at
least one miRNA is down-regulated, relative to the signal generated
from the control sample. In some embodiments, the microarray
comprises miRNA-specific probe oligonucleotides for a substantial
portion of all known human miRNAs. In a further embodiment, the
microarray comprises miRNA-specific probe oligonucleotides for one
or more miRNAs selected from the group consisting of SEQ ID NOs:
763-1350, or 1565 and any combination thereof.
[0084] The microarray can be prepared from gene-specific
oligonucleotide probes generated from known miRNA sequences. The
array may contain two different oligonucleotide probes for each
miRNA, one containing the active, mature sequence and the other
being specific for the precursor of the miRNA. The array may also
contain controls, such as one or more mouse sequences differing
from human orthologs by only a few bases, which can serve as
controls for hybridization stringency conditions. tRNAs and other
RNAs (e.g., rRNAs, mRNAs) from both species may also be printed on
the microchip, providing an internal, relatively stable, positive
control for specific hybridization. One or more appropriate
controls for non-specific hybridization may also be included on the
microchip. For this purpose, sequences are selected based upon the
absence of any homology with any known miRNAs.
[0085] The microarray may be fabricated using techniques known in
the art. For example, probe oligonucleotides of an appropriate
length, e.g., 40 nucleotides, are 5'-amine modified at position C6
and printed using commercially available microarray systems.
Labeled cDNA corresponding to the target RNA sequence(s) is
prepared by reverse transcribing the target RNA with labeled
primer. Following first strand synthesis, the RNA/DNA hybrids are
denatured to degrade the RNA templates. The labeled target cDNAs
thus prepared are then hybridized to the microarray chip under
typical hybridizing conditions. At positions on the array where the
immobilized probe DNA recognizes a complementary target cDNA in the
sample, hybridization occurs. The labeled target cDNA marks the
exact position on the array where binding occurs, allowing
automatic detection and quantification. The output consists of a
list of hybridization events, indicating the relative abundance of
specific cDNA sequences, and therefore the relative abundance of
the corresponding complementary miRNAs, in the patient sample.
[0086] In an aspect, the disclosure relates to kits. Such kits can
be used in methods of identifying a miRNA or mRNA described herein;
an expression level or expression pattern of one or more miRNA(s)
or mRNA(s) described herein; and/or identifying a B-cell
malignancy. In some embodiments the kit can provide a diagnosis of
a B-cell type and or a B-cell malignancy. In some embodiments the
kit can differentiate one B-cell malignancy from other B-cell
malignancies (e.g., ABC-DLBCL from GBC-DLBCL), and can provide
information useful to a medical professional regarding a preferred
course of therapeutic treatment. Suitably, a kit can comprise an
isolated nucleic acid molecule or a plurality of isolated nucleic
acid molecules as described herein (e.g., a sequence complementary
to any of the miRNAs disclosed in the Tables). In embodiments, the
isolated nucleic acid molecule can comprise a sequence of one or
more RT-PCR target sequences, primers directed thereto, or a
sequence complementary thereto. The kit can also include adapter
nucleic acid molecules (e.g., universal adapter molecules for
attachment to expressed miRNAs/mRNAs for reverse transcription and
amplification); appropriate buffer systems and reagents, detectable
labels, an energy source (e.g., ATP), and other agents and
components that can be used in performing analysis of miRNA
expression (e.g., in RT-PCR, deep sequencing, or microarray-based
methods). Kits also include instructions for use.
[0087] It will be understood that any numerical value recited
herein includes all values from the lower value to the upper value.
For example, if a concentration range is stated as 1% to 50%, it is
intended that values such as 2% to 40%, 10% to 30%, or 1% to 3%,
etc., are expressly enumerated in this specification. These are
only examples of what is specifically intended, and all possible
combinations of numerical values between the lowest value and the
highest value enumerated are to be considered to be expressly
stated in this application.
[0088] In an aspect, the disclosure provides a DNA library
comprising one or more miRNA sequences from Tables 4-5, or 6-33. In
a related aspect, the disclosure provides a method for generating
such a DNA library. In an embodiment the library comprises a cDNA
library that includes sequences derived from a sample of the miRNAs
or, in addition or alternatively, the mRNA purified from a
particular source such as, for example, a collection of cells, a
particular tissue, or an entire organism. In embodiments, the
source of the cDNA library is a B cell, such as a B cell in any
stage (e.g., naive, germinal center, memory, activated, or plasma,
etc.) or a B cell malignancy (e.g., mantle cell lymphoma,
follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, germinal
center B-cell like diffuse large B cell lymphoma (DLBCL), chronic
lymphocytic leukemia, small lymphocytic lymphoma, lymphoplasmacytic
lymphoma, multiple myeloma, and activated B-cell like DLBCL).
Typically, the isolated miRNA (or mRNA) is converted to a DNA
template by reverse transcription, and comprises the cDNA version
of the expressed RNA (e.g., miRNA or mRNA). Thus, a library can
represent the cDNA version of the active "transcriptome" in a
particular source under the physiological, developmental, or
environmental conditions that existed when the miRNA/mRNA was
purified.
[0089] In an embodiment, the library comprises a miRNA sequence
described in Tables 4-5; 7-33. In embodiments, the library
comprises at least one of SEQ ID NOs 763-1350 or 1565, and any
combination thereof. In an embodiment, the library comprises a
collection of miRNA sequences comprising SEQ ID NOs 763-1350 or
1565. In embodiments the library can be used to identify and/or
differentiate a B-cell malignancy from other B-cell malignancies.
In such embodiments, the library comprises at least one miRNA
sequence selected from those listed in any of Tables 4 or 7-35.
[0090] As used herein, a "library" is a collection of DNA sequences
that is stored and propagated in a population of microorganisms
through standard molecular cloning processes. A DNA library can be
of any type such as, for example, a cDNA library (formed from
reverse-transcribed RNA) or a genomic library (formed from genomic
DNA). The DNA library can be used in any routine application or
technique known in the art (e.g., gene discovery; cloning of
full-length cDNAs to identify/study gene function; miRNA/mRNA
expression in different cells or tissues; splice variants in
different cells or tissues) and, in some embodiments, can depend on
the source of the original DNA fragments. In embodiments, the
library can be used to isolate, characterize, and/or quantify the
actively expressed miRNA is a population of cells such as, for
example, B-cells or B-cell malignancies. In some embodiments, the
library can be used to study miRNA-protein interactions or
miRNA-based regulation of protein expression or activity.
[0091] Any known method of library preparation can be used to make
the library described herein, including the methods described in
the detailed description and non-limiting Examples. Further general
techniques can be based on the methods and techniques known in the
art, (see, e.g., RNA Methodologies: A Laboratory Guide for
Isolation and Characterization (R. E. Farrell, Academic Press,
1998); cDNA Library Protocols (Cowell & Austin, eds., Humana
Press; Functional Genomics (Hunt & Livesey, eds., 2000); and
the Annual Review of Genomics and Human Genetics (E. Lander, ed.,
yearly publications by Annual Reviews). Suitably, the nucleotide
sequences of interest in a library are preserved as inserts in a
plasmid or the genome of a bacteriophage that has been used to
infect bacterial cells. There are differences in the cloning
vectors and techniques used in library preparation, but in general
each DNA fragment is uniquely inserted into a cloning vector and
the pool of recombinant DNA molecules is then transferred into a
population of bacteria or yeast such that each organism contains on
average one construct (vector+insert). The DNA molecules are copied
and propagated along with the population of organisms in culture
(thus, effectively, "cloned"). Accordingly, in some embodiments,
the term "library" can refer to a population of organisms, each of
which carries a DNA molecule inserted into a cloning vector, or
alternatively to the collection of all of the cloned vector
molecules.
[0092] An "increased level" of expression, as used herein, refers
to a level of expression that is at least 2-fold greater than the
level of expression in a control cell type or tissue. In various
embodiments, the level of expression is at least 2.5-fold, at least
3-fold, at least 5-fold, or at least 10-fold, greater than the
level of expression in a control cell. Exemplary control cells and
tissues include, but are not limited to, normal cells, benign lymph
nodes, and other B cell malignancies. In certain embodiments,
benign lymph nodes are used as a control tissue. Such benign lymph
node tissue contains a variety of cell types.
[0093] A "decreased level" of expression, as used herein, refers to
a level of expression that is less than 50% of the level of
expression in a control cell.
[0094] The term "differentially expressed" or "differential
expression" relates to a difference in the observed or detected
level of expression of a biomolecule such as, for example, nucleic
acids (e.g., a polynucleotide, mRNA, miRNA, etc.) or amino acid
sequence (e.g., protein, polypeptide, etc.) in a test sample
relative to the observed or detected level of expression of the
same biomolecule in a control sample or other reference (e.g., a
previously established reference level). The difference in
expression can be either an increase or a decrease in the
expression of the biomolecule in the test sample relative to the
control sample.
[0095] The Examples that follow provide further illustration of
certain aspects and embodiments described in the foregoing
description. These illustrative Examples should not be interpreted
as limiting the scope of the appended claims.
EXAMPLES
Example 1
Materials and Methods
[0096] Patient Sample Processing
[0097] B cell populations were obtained from young patients
undergoing routine tonsillectomy using a protocol approved by the
Clinical Center at the National Institutes of Health. Patient
tonsils were disaggregated and separated by Ficoll. The mononuclear
cell layer was harvested, washed in PBS, and resuspended in ACK
lysing buffer to remove small numbers of red blood cells. After a
wash and resuspension with 10 ml of PBS with 10% Bovine Serum
Albumin, cells were counted and 200 million were stained with
fluorochrome-tagged monoclonal antibodies to CDI9, IgD, CD38 and
CD27. The specific monoclonal antibodies employed were
anti-CDI9-PE-Cy5.5, anti-IgD-FITC, anti-CD27-PE, and anti-CD38-APC,
all from BD Biosciences and BD Pharmingen (San Jose Calif.). Cells
were sorted using the MoFlo Cell sorter (Dako Cytomation, Colorado
Springs, Colo.) into naive B cells (CDI9+IgD+CD2TCD38+), germinal
center B cells (CDI9+IgD-CD38++), memory B cells
(CDI9+IgD-CD27+CD38dim) and plasma cells
(CDI9dimIgD-CD27++CD38+++). Three replicates of each B cell subset
were obtained from separate patients. The sample purity was
verified by FACS and found to be over 90% in all cases.
[0098] Tumor specimens were obtained from patients who were
examined under a protocol approved by the Duke University Medical
Center Institutional Review Board. The pathologic diagnosis of the
samples was verified prior to analysis. Samples from patients with
diffuse large B cell lymphoma were further subclassified as
described previously. See Hans et al. (2004) Blood 103: 275-282.
Chronic lymphocytic leukemia samples were processed and purified as
described previously. See Volkheimer et al. (2007) Blood 109:
1559-1567. Total RNA was extracted using the phenol-chloroform
method to preserve miRNAs, using Ambion reagents.
[0099] microRNA Profiling Using Multiplexed Real-Time PCR
[0100] MiRNA expression profiling was conducted using the Applied
Biosystems 384-well multiplexed real-time PCR assay using 400 ng of
total RNA. Eight reactions, each containing 50 ng of RNA and a
multiplex looped primer pool with endogenous small nucleolar
(sno)-RNA controls, were used to reverse-transcribe the miRNAs in
parallel fashion. Each completed reaction was loaded onto the
384-well plate per manufacturer's instructions, and real-time PCR
was run on the ABI 7900HT Prism. For each 384-well plate, we used
the automatically determined cycle-threshold (CT) using the SDS
2.2.1 software (Applied Biosystems). Consistent with manufacturer
recommendations, we considered CT greater than 36 as undetected. A
miRNA was considered to be present in a subpopulation if the CT was
less than 36 in all three biological replicates. The probes deemed
to be present were normalized to the average expression of a
sno-RNA control. The expression values were calculated as
2.sup.-.DELTA.CT, then median centered to 500 and log
2-transformed.
[0101] Gene Expression (mRNA) Profiling Using Microarrays
[0102] Gene expression profiling and normalization were performed
using methods identical to those we have described previously. See
Dave et al. (2004) N. Engl. J. Med. 351: 2159-2169. Array elements
with median signal intensities of less than 7 log 2 units across
the samples were removed from analysis, in order to exclude poorly
measured genes and genes not appreciably expressed in the samples.
Genes that were on-average 2-fold or higher differentially
expressed in a binary comparison of B cell subsets, and appreciably
expressed in at least one of the 2 B cell subsets being compared,
were selected for further analysis as described below. The data
have been deposited in the publicly available Gene Expression
Omnibus database (GSE12366).
[0103] MiRNA Profiling Using Microarray
[0104] MiRNA expression profiling from human B cell malignancies
was conducted using up to 1 .mu.g of total RNA from sample and
reference (normal lymph node), which were labeled with Cy3 or Cy5
fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon,
Denmark). The fluorescently labeled samples were combined and
hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a
nitrogen atmosphere. The micro array slides were scanned with
GenePix 4100 Scanner. The quantified signals were normalized using
the global Lowess algorithm, using Genespring (Agilent) software.
The intensity values for multiple spots were averaged and the
normalized values were log 2-transformed. Missing values were
replaced with the lowest value for analysis.
[0105] MiRNA Target Prediction
[0106] Annotated genes on the U133plus 2.0 array were matched to
the miRNA target list downloaded from TargetScan
(www.targetscan.org). For the purpose of this study, a target gene
was defined by the presence of a seed sequence match (nucleotides
2-8) and conservation of the seed sequence and 3 'UTR in humans,
dog, rat, mouse and chicken. Additional conservation was examined
in miRNA target genes selected for experimental validation. The
distribution of the mRNA expression for these genes was plotted as
a density plot using the Splus statistical software (Insightful
Corporation). The difference in distribution between the B cell
subsets was calculated using a two-sample, 1-sided
Kolmogorov-Smirnov test to examine the hypothesis that being a
miRNA target conferred repression in the appropriate population
(consistent with the known biology of miRNA effects).
[0107] The 3'UTRs of LM02, MYBL1 and PRDM1 were aligned using
Blastz alignment of Human, Chimp, Mouse, Rat, Dog, Chicken, Frog
(Xenopus) and Zebrafish, and were displayed using the UCSC genome
browser. The conservation of miR-223 seed sequence and the 3'UTRs
of LM02 and MYBL1, as well as that of the miR-30 family and miR-9
on PRDM1, were thus verified.
[0108] Western Blot
[0109] RIPA Lysis buffer (1.times. phosphate-buffered saline [PBS],
1 Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 10 mM
phenylmethylsulfonyl fluoride, 1 .mu.g/mL aprotinin, and 100 mM
sodium orthovanadate) was added to 750,000 cells and incubated on
ice for 30 minutes. The mixture was spun down and the supernatant
was transferred to a new tube as the whole cell extract. A total of
20 .mu.g of cell lysate was separated on a 4-18% Tris-Bis NuPAGE
gel (Invitrogen) and transferred using the iBlot transfer device
(Invitrogen) program 3 for 7 minutes (LM02 detection) or program 2
for 6 minutes (PRDM1). The blots were probed using 1:200
mouse-anti-LM02 (Santa Cruz Biotechnologies SC-65736), 1:750
mouse-anti-Blimp-1 (Santa Cruz Biotechnologies SC-66015) or 1:5000
goat-anti-B-actin (Santa Cruz Biotechnologies SC-47778) for 1 hour
at room temperature. The antibodies were detected using 1:10,000
goat-anti-mouse horseradish peroxidase conjugated antibodies (Santa
Cruz Biotechnologies). Western Blotting Luminol Reagent (Santa Cruz
Biotechnologies) was used to visualize the bands corresponding to
each antibody.
[0110] Single miRNA/mRNA Expression Using Real-Time Polymerase
Chain Reaction (RT-PCR)
[0111] With 10 ng of RNA per reaction, miRNAs of interest were
reverse-transcribed with ABI individual stem-loop primers designed
to detect only mature miRNA, and measured by Taqman real-time PCR
normalized to the small nucleolar RNA, RNU48. In order to assess
mRNA expression using RT-PCR, 1 .mu.g of RNA was
reverse-transcribed with the ABI High Capacity cDNA Reverse
Transcription kit. Gene expression was measured with exon-spanning
Taqman probes, and normalized to beta-2 micro globulin
expression.
[0112] Cell Culture
[0113] BJAB and H929 were cultured in RPMI (Gibco) supplemented
with 10% fetal bovine serum, and U266 was cultured in RPMI
supplemented with 15% fetal bovine serum. 293T cells were grown in
DMEM media (Gibco) with 10% FBS. All cell lines were grown in
37.degree. C. humidified cell culture incubators with CO.sub.2
maintained at 5%.
[0114] MiRNA Functional Analysis
[0115] MicroRNA Transfection
[0116] miRNAs of interest were over-expressed in cell lines of
interest by transfecting the appropriate miRNA precursors (Ambion)
at 100 nanomoles using Amaxa's Nucleofector system. In particular,
BJAB was transfected with Nucleofector solution T, program T-016,
U266 with Nucleofector C, program X-005, and H929 with Nucleofector
V, program T-001. 1.5 million cells were used per transfection and
mixed with appropriate miRNA precursors (Ambion) for a
concentration of 100 nM.
[0117] Statistical Analysis
[0118] Identifying Differentially Expressed miRNA and mRNA
[0119] MiRNAs were considered to be differentially expressed if the
mean signal was changed at least 2-fold and a false discovery rate
(q) was less than 5% using Significance Analysis of Microarrays
(SAM) with 1000 permutations. See Tusher et al. Proc Natl Acad Sci
USA. 2001; 98:5116-5121.
[0120] Differentially expressed genes (mRNA) in Naive versus
Germinal Center, Germinal Center versus Plasma Cells, and Germinal
Center versus Memory Cells comparisons were identified using SAM.
Genes that were 2-fold differentially expressed at a false
discovery rate (q) less than 1% with 1000 permutations were
identified as significantly differentially expressed.
[0121] Transcription Factors and miRNA Target Genes
[0122] Transcription factors were identified based on the gene
ontology (GO search term "transcription factor") and matched to the
probes of the Affymetrix U133plus 2.0 microarray. Of the total of
938 transcription factor genes thus identified, we selected 364
genes that were differentially expressed in at least one of the B
cell stage transitions. We evaluated the breakdown of the
differentially expressed transcription factors among miRNA targets
versus non targets. The p-values were computed using a chi-square
test separately in each B cell stage-transition.
[0123] B Cell Malignancy Sample Classification
[0124] The top 50 most differentially expressed miRNAs (P<0.01)
in each pair-wise B cell malignancy type comparison were chosen as
the initial predictor. Singular value decomposition was applied to
reduce the list to 20 most informative miRNAs in each pair-wise
comparison. See West et al. Proc Natl Acad Sci USA. 2001;
98:11462-11467. A Bayesian logistic regression was performed in
Matlab (Mathworks) using the 20-predictor miRNAs for each pair-wise
comparison. Each sample was tested using the microRNA-based
predictor in a leave-one-out fashion to determine the accuracy of
each prediction. For a sample to be classified as a particular B
cell malignancy (or normal) type, it had to be predicted as such in
every pair-wise comparison.
[0125] Normal B Cell Stage Classification of B Cell
Malignancies
[0126] We constructed a Bayesian predictor to distinguish normal
naive from germinal center B cells based on the 32 miRNAs depicted
in FIG. 1D. We then applied the predictor without optimization to
the microarray data generated for GCB DLCBL, Burkitt lymphoma and
chronic lymphocytic leukemia to render a Bayesian prediction of
lineage; i.e. naive versus germinal center B cell.
[0127] Western Blot Quantitative Analysis
[0128] Western blot scans were quantified using NIH ImageJ
software. For each experiment, the ratios of protein of interest
(LM02, PRDM1) to Actin were determined and mean centered to 100
across the experiment. The average and standard deviation of these
values across the three experiments were calculated and displayed
relative to the scrambled control expression.
[0129] Luciferase Indicator Assay Quantitative Analysis
[0130] Firefly luciferase reporter constructs were created in the
pL/SV40/GL3 vector for the LM02 3'UTR and the LM02 3'UTR with the
predicted miR-223 binding site mutated, as described below. Mature
microRNA expression of a pL/CMV/eGFP vector coding for pri-miR-223
from the 3'UTR of EGFP of the vector was confirmed by Taqman-real
time PCR in transfected 293T cells. gl3 activity was normalized in
dual luciferase assays to pL/SV40/RLuc, with which it was
cotransfected. The PRDM1 3'UTR was also cloned into the pL/SV40/GL3
vector. microRNA expression vectors and their respective seed
sequence mutants were created for miR-9-2, miR-30b, and
miR-30d.
[0131] LM02
[0132] The LM02 3'UTR was PCR-amplified from BJAB cDNA using
primers 1 and 2 (SEQ ID NOs: 1 and 2, respectively) and ligated
into the XhoI and XbaI sites of the previously described lentiviral
vector pL/SV40/GL3, which expresses firefly luciferase. See Tusher
et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121. As a control, an
LM02 3'UTR mutant was created using mutant PCR primers 3 and 4 and
then outer primers 1 and 2. The resulting fragment was also placed
into the XhoI and XbaI sites of pL/SV40/GL3. In this LM02 3'UTR
mutant, the seed match predicted to bind to nucleotides 2-8 of
miR-223 is converted from 5'AACUGAC 3' to 5'AACAGUC 3'. To create a
miR-223 expression vector, a .about.350 nucleotide-long fragment of
pri-miR-223, encompassing the pre-miRNA stem loop in its middle,
was PCR-amplified from genomic BJAB DNA with primers 6 and 7 (SEQ
ID NOs. 6 and 7) and ligated into the XhoI and XbaI sites of the
pL/CMV/eGFP vector. This pL/CMV/eGFP vector was generated by
ligating a fragment containing the CMV promoter and the EGFP ORF
into the BamHI and XhoI sites of the previously described
lentiviral backbone pL. See Tusher et al. Proc Natl Acad Sci USA.
2001; 98:5116-5121. The expression of miR-223 from the 3'UTR of
EGFP in the resulting vector was confirmed by Taqman real time PCR
in transfected 293T cells.
[0133] For luciferase indicator assays, 293T cells plated in 24
well plates were transfected using FUGENE6 as follows:
TABLE-US-00001 TABLE 1 Luciferase indicator assay compositions 2.5
ng pL/SV40/GL3 2.5 ng pL/SV40/GL3/ 2.5 ng pL/SV40/GL3/ 2.5 ng
pL/SV40/RLuc LMO2 LMO2 seed 0.4 .mu.g pL/CMV/ UTR mut eGFP/miR-223
2.5 ng pL/SV40/RLuc 2.5 ng pL/SV40/RLuc 2.5 ng pL/SV40/GL3 0.4
.mu.g pL/CMV/ 0.4 .mu.g pL/CMV/eGFP/ 2.5 ng pL/SV40/RLuc
eGFP/miR-223 miR-223 0.4 .mu.g pL/CMV/eGFP 2.5 ng pL/SV40/ 2.5 ng
pL/SV40/GL3/ GL3/LMO2 LMO2 seed UTR mut 2.5 ng pL/SV40/RLuc 2.5 ng
pL/SV40/RLuc 0.4 .mu.g pL/CMV/eGFP 0.4 .mu.g pL/CMV/eGFP
[0134] Reporter expression was evaluated by dual luciferase assays
(Promega) 48 hours post-transfection. Firefly Luciferase (GL3) to
internal control Renilla Luciferase (RLuc) ratios from 293T cells
transfected with pL/CMV/eGFP/miR-223 were divided by those obtained
from 293T transfected with the pL/CMV/eGFP vector control. The
average and standard deviation were taken across five experiments
for the pL/SV40/gl3 empty, LM02, and LM02 mutant vectors.
[0135] Firefly Luciferase (GL3) activity readings of the PRDM1
3'UTR construct were divided by internal control Renilla Luciferase
(RLuc) activity readings. The average and standard deviation of
these ratios across three experiments were calculated and scaled
relative to the empty vector (pL/CMV/eGFP) transfection.
TABLE-US-00002 TABLE 2 LM02 primer sequences Primer SEQ ID
description Sequence (5' .fwdarw. 3') 1543 LMO2 3'UTR, FW
ATATCTCGAGGCCCGAGTCCCCGGGCATCTTTGG 1544 LMO2 3'UTR, REV
ATATATCTAGACTACACACGACAAATACTTTG 1545 LMO2 3'UTR seed
CAGCCCATCCATAGTAACAGTCATGATTAGCAGAAGAAAGG mutant, FW 1546 LMO2
3'UTR seed CCTTTCTTCTGCTAATCATGACTGTTACTATGGATGGGCTG mutant, REV
1547 pri-mir-223, FW ATATCTCGAGGGTCACATCTCCCAGGAAGATC 1548
pri-mir-223, REV ATATATCTAGAAGCACTCTCATGGTGTGTGTAG
[0136] PRDM1
[0137] The PRDM1 3'UTR was PCR-amplified from BJAB genomic DNA in
two reactions using primer pairs 7 and 8 in one reaction, and 9 and
10 in another (SEQ ID NOs: 7 to 10, respectively). The two
fragments were then ligated together into the XhoI and NotI sites
of pL/SV40/GL3 to generate the entire PRDM1 3'UTR. microRNA
expression vectors were created as described above for mir-9-2,
mir-30b, and mir-30d with the primers listed below. Again,
over-expression from the 3'UTR of EGFP in the resulting vector was
confirmed by Taqman real-time PCR in transfected 293T cells. For
the PRDM1 luciferase assays, we used the sequences listed below to
PCR-mutate the seed sequence of the microRNA expression vectors
rather than the PRDM1 3'UTR, which had numerous seed sequence
binding sites.
TABLE-US-00003 TABLE 3 PRDM1 primer sequences Primer SEQ ID:
description sequence 1549 PRDM1 3'UTR piece 1, FW
AGAGACTCGAGGATTTTCAGAAAACACTTATTT 1550 PRDM1 3'UTR piece 1, REV
TTGCTTCTCTAGAGGAGAAAC 1551 PRDM1 3'UTR piece 2, FW
GTTTCTCCTCTAGAGAAGCAA 1552 PRDM1 3'UTR piece 2, REV
AGAGAGCGGCCGCAGGGGAGAGACAAATTGCATTG 1553 pri-mir-9-2, FW
AGAGACTCGAGATAAAAGGAGGAATCTTAAG 1554 pri-mir-9-2, REV
AGAGAGCGGCCGCGAAAAAAACAAAACAAAAACAA 1555 pri-mir-30b, FW
AGAGAGCGGCCGCCCGATTGAGTCTTGCCTCAT 1556 pri-mir-30b, REV
AGAGAGAATTCAATGGTCTCACATTTCCAAC 1557 pri-mir-30d, FW
AGAGAGCGGCCGCATGTCACAGCTATTGTTCAG 1558 pri-mir-30d, REV
AGAGAGAATTCGCAGTAAAAGAATGCAGCTA 1559 pri-mir-9-2 seed mutant, FW
GGAAGCGAGTTGTTATCTATGCTTATCTAGCTGTATGAGT 1560 pri-mir-9-2 seed
mutant, REV ACTCATACAGCTAGATAAGCATAGATAACAACTCGCTTCC 1561
pri-mir-30b seed mutant, FW
ACCAAGTTTCAGTTCATGTTAAGATCCTACACTCAGCTGT 1562 pri-mir-30b seed
mutant, REV ACAGCTGAGTGTAGGATCTTAACATGAACTGAAACTTGGT 1563
pri-mir-30d seed mutant, FW
CAGAAAGTCTGTTGTTGTTAAGATCCCCGACTGGAAGCTG 1564 pri-mir-30d seed
mutant, REV CAGCTTCCAGTCGGGGATCTTAACAACAACAGACTTTCTG
[0138] Luciferase assays were carried out in a manner similar to
those described for LMO2.
[0139] IgVH Mutation Status of Chronic Lymphocytic Leukemia
Samples
[0140] IgVH mutation status was determined as described in
Volkheimer et al. (Blood. 2007; 109:1559-1567) using genomic DNA.
In brief, genomic DNA was isolated from purified CLL cells and
isolated using the GenElute Mammalian DNA extraction Kit from Sigma
(St. Louis, Mo.) according to the manufacturer's instructions. DNA
was amplified using nested PCR primers. PCR products were
electrophoresed, purified, and sequenced using an automated DNA
sequencer (Applied Biosystems, Foster City, Calif.) with the BigDye
Terminator kit (Perkin Elmer, Boston, Mass.). Forward and reverse
sequences were aligned into a single resolved sequence using
Sequencher 4.1 software (Gene Codes Corporation, Ann Arbor, Mich.),
and then aligned with germline sequences derived from DNA Plot on
the V BASE directory website (http://vbase.mrc-cpe.cam.ac.uk/). The
percent sequence identity was calculated by dividing the number of
mutations from FR1 to FR3 by the total number of nucleotides in
this region. Samples were considered somatically mutated if they
had greater than 2% mutations in this region.
Example 2
Mature B Cell Stages Display Characteristic Patterns of MicroRNA
Expression
[0141] Mature B cell subsets can be defined by the expression of
surface CD19, IgD, CD38, and CD27, and were obtained by
fluorescence activated cell sorting of tonsils from young
individuals undergoing routine tonsillectomy. See FIGS. 1B and 1C.
Cells were previously gated on CD19 positive cells. Naive and
memory B cells were distinguished from germinal center and plasma
cells based on surface CD38 and IgD expression.
[0142] To determine whether mature B cell subsets had unique
patterns of microRNA (miRNA) expression, we used a 384-well
multiplexed real time polymerase chain reaction (RT-PCR) assay
(Applied Biosystems) that allowed measurement of all 365 miRNAs in
miRBase 9.2. See Chen et al. Nucleic Acids Res. 2005; 33:e179; and
He et al. Nature. 2007; 447:1130-1134. We detected a total of 113
unique miRNAs in the B cell populations. See Table 4. This
detection frequency compares favorably to the identification of 71
unique miRNAs (45 miRNAs with more than one clone) through the
examination of 3101 sequences cloned from unselected CD19-positive
mature B cells. See Landgraf et al. Cell. 2007; 129:1401-1414. We
identified differentially expressed miRNAs in mature B cell subsets
using a false discovery rate of less than 5%. See FIGS. 1D, 1F, and
1H. The complete list of assayed miRNAs found to be expressed in
the B cell populations is shown in Tables 4 and 5.
[0143] In Table 4, normalized expression values for B-cell subsets
are shown, along with significance analysis of microarrays q
values. Empty microRNA expression cells indicate below-threshold
values, defined as RT-QPCR Ct values greater than 36 or undetected.
SAM q values greater than 5 were considered non-significant and not
displayed.
TABLE-US-00004 TABLE 4 MicroRNAs detected in at least one B-cell
subset qNaive qGerminal qGerminal Germinal vs Center Center Naive
Center Plasma Cell Memory Germinal vs vs average average average
average Center Plasma Cell Memory hsa-let-7a 11.7 9.2 11.2 1.4
hsa-let-7b 10.3 9.0 7.7 11.3 0.0 hsa-let-7c 7.3 7.2 hsa-let-7d 9.0
9.0 10.0 hsa-let-7f 10.3 7.6 8.4 10.2 0.0 hsa-let-7g 13.1 10.3 10.5
14.0 0.0 0.0 hsa-miR-100 7.4 hsa-miR-101 8.4 10.0 hsa-miR-103 9.7
11.4 9.8 10.8 0.0 0.0 hsa-miR-106b 11.7 12.5 11.6 12.7 hsa-miR-125a
6.7 8.3 hsa-miR-125b 6.3 7.3 hsa-miR-130b 7.8 9.8 8.6 10.2 3.6 4.5
hsa-miR-132 7.5 8.9 hsa-miR-133b 7.7 7.7 7.2 7.3 hsa-miR-140 10.8
12.0 11.1 12.0 4.7 hsa-miR-141 7.0 6.2 hsa-miR-142-3p 16.9 17.0
15.3 18.0 1.1 hsa-miR-142-5p 11.7 11.0 9.8 13.4 2.1 hsa-miR-146a
10.1 14.0 14.0 14.6 0.0 hsa-miR-146b 9.2 9.2 8.1 10.5 2.1
hsa-miR-148a 6.7 9.4 11.9 9.0 0.0 hsa-miR-148b 6.4 8.5 hsa-miR-151
8.2 9.6 6.6 9.0 1.5 0.0 hsa-miR-152 6.4 9.7 6.6 hsa-miR-155 14.2
14.6 15.0 15.6 hsa-miR-15a 10.2 10.1 7.8 10.8 2.0 hsa-miR-15b 11.2
14.0 12.6 12.9 0.0 2.8 hsa-miR-16 16.7 17.3 17.1 17.1 hsa-miR-17-3p
9.1 hsa-miR-17-5p 8.8 11.5 8.9 9.4 0.0 0.0 0.0 hsa-miR-181b 9.0
11.7 10.8 9.3 0.0 0.0 hsa-miR-181d 8.2 10.6 8.5 9.0 0.0 1.1
hsa-miR-182 8.7 8.7 hsa-miR-186 8.8 9.2 8.7 9.7 hsa-miR-18a 5.7 8.9
6.8 7.4 0.0 0.0 hsa-miR-191 11.9 12.7 12.4 13.3 hsa-miR-192 9.1 9.0
8.8 10.1 hsa-miR-193b 6.6 hsa-miR-194 6.8 7.4 4.9 9.5 2.0 4.7
hsa-miR-195 10.3 8.7 8.8 9.2 hsa-miR-197 9.7 10.5 8.7 11.0 0.0
hsa-miR-199a* 7.0 hsa-miR-19a 9.7 12.8 11.9 11.1 0.0 hsa-miR-19b
14.0 15.6 14.9 15.0 0.8 hsa-miR-200a 6.5 6.8 hsa-miR-200b 7.5
hsa-miR-200c 9.0 9.6 9.7 hsa-miR-203 7.9 7.6 hsa-miR-20a 12.5 14.9
12.2 13.8 0.0 0.0 hsa-miR-20b 8.4 11.0 8.0 8.5 0.0 1.1 0.0
hsa-miR-21 12.6 13.0 11.2 15.1 2.8 2.1 hsa-miR-210 9.2 11.1 10.7
10.2 0.8 hsa-miR-214 7.6 hsa-miR-22 8.3 7.4 hsa-miR-221 6.9
hsa-miR-222 11.4 11.6 7.8 12.7 0.0 4.7 hsa-miR-223 13.7 10.4 9.6
14.6 0.0 0.0 hsa-miR-23a 8.7 hsa-miR-23b 6.2 hsa-miR-24 11.7 11.8
12.8 13.4 2.1 hsa-miR-25 9.7 10.8 8.8 11.2 2.8 hsa-miR-26a 14.7
13.6 14.2 16.3 0.0 hsa-miR-26b 12.6 12.1 12.0 14.1 3.4 hsa-miR-27a
6.5 11.1 0.0 hsa-miR-27b 7.2 hsa-miR-28 9.4 13.3 8.4 10.3 0.0 0.0
0.0 hsa-miR-296 6.9 8.1 6.6 7.9 0.0 2.0 hsa-miR-29a 13.4 11.7 13.5
15.0 0.0 hsa-miR-29c 11.2 8.7 12.3 13.3 1.5 2.6 0.0 hsa-miR-301 6.0
9.6 7.7 7.9 0.0 0.0 hsa-miR-30a-3p 8.2 hsa-miR-30a-5p 11.9 12.6
11.3 13.0 2.0 hsa-miR-30b 11.7 12.6 11.2 13.0 2.8 hsa-miR-30c 13.0
13.9 12.7 14.3 4.5 hsa-miR-30d 10.8 11.9 10.1 12.1 3.8 0.0
hsa-miR-30e-3p 8.3 9.0 6.8 9.9 0.0 hsa-miR-30e-5p 12.5 8.1 10.2
14.8 0.0 hsa-miR-31 9.0 hsa-miR-32 8.4 7.0 10.5 hsa-miR-320 10.1
8.2 10.3 10.5 1.5 2.6 0.0 hsa-miR-324-3p 8.8 9.9 9.0 9.5
hsa-miR-324-5p 8.0 7.8 hsa-miR-328 8.1 8.6 7.4 8.7 2.8 hsa-miR-331
9.4 12.6 12.0 10.8 0.0 2.6 hsa-miR-335 6.3 6.9 hsa-miR-339 7.3 5.7
8.9 hsa-miR-342 12.7 12.5 10.6 13.1 0.0 hsa-miR-345 6.8 7.1 7.8 8.6
3.4 hsa-miR-361 8.3 hsa-miR-365 6.0 7.8 8.3 8.4 0.0 hsa-miR-374 8.0
8.9 10.4 9.8 hsa-miR-423 8.0 9.7 7.6 8.7 0.0 0.0 hsa-miR-425 6.9
hsa-miR-425-5p 8.7 11.6 9.7 10.2 0.0 0.0 hsa-miR-484 11.5 12.3 12.9
12.0 hsa-miR-486 7.0 8.8 9.9 9.6 3.8 hsa-miR-532 7.0 7.8
hsa-miR-545 5.5 6.3 hsa-miR-572 7.7 6.8 hsa-miR-629 6.7 7.3 7.2 9.0
1.4 hsa-miR-646 6.0 7.3 hsa-miR-650 8.8 11.4 8.0 hsa-miR-659 7.6
6.0 6.8 hsa-miR-660 9.5 8.6 10.1 hsa-miR-7 6.1 5.8 hsa-miR-9 7.8
6.8 6.2 2.8 hsa-miR-9* 6.4 6.5 hsa-miR-92 14.5 15.8 14.2 15.5 3.6
0.0 hsa-miR-93 11.5 14.6 10.5 12.3 0.0 0.0 0.0 hsa-miR-98 6.3 5.0
5.0 7.8 2.1 hsa-miR-99a 7.3 hsa-miR-99b 6.8
TABLE-US-00005 TABLE 5 miRNAs measured using the multiplex RT-PCR
assay, but not consistently detected in any B-cell population
hsa-let-7e hsa-miR-383 hsa-miR-376a hsa-miR-566 hsa-miR-I0a
hsa-miR-198 hsa-miR-376b hsa-miR-551b hsa-miR-I0b hsa-miR-224
hsa-miR-380-5p hsa-miR-569 hsa-miR-34a hsa-miR-299-5p hsa-miR-410
hsa-miR-570 hsa-miR-34b hsa-miR-409-5p hsa-miR-412 hsa-miR-548a
hsa-miR-34c hsa-miR-432 hsa-miR-432 hsa-miR-586 hsa-miR-107
hsa-miR-433 hsa-miR-512-5p hsa-miR-587 hsa-miR-181c hsa-miR-485-5p
hsa-miR-199a hsa-miR-548b hsa-miR-215 hsa-miR-489 hsa-miR-199b
hsa-miR-588 hsa-miR-218 hsa-miR-494 hsa-miR-219 hsa-miR-589
hsa-miR-372 hsa-miR-506 hsa-miR-323 hsa-miR-550 hsa-miR-375
hsa-miR-508 hsa-miR-338 hsa-miR-591 hsa-miR-378 hsa-miR-521
hsa-miR-368 hsa-miR-593 hsa-miR-137 hsa-miR-134 hsa-miR-373
hsa-miR-596 hsa-miR-200a hsa-miR-147 hsa-miR-373 hsa-miR-597
hsa-miR-I hsa-miR-149 hsa-miR-382 hsa-miR-622 hsa-miR-183
hsa-miR-153 hsa-miR-424 hsa-miR-599 hsa-miR-302a hsa-miR-187
hsa-miR-448 hsa-miR-600 hsa-miR-302c hsa-miR-190 hsa-miR-450
hsa-miR-624 hsa-miR-302d hsa-miR-193a hsa-miR-451 hsa-miR-601
hsa-miR-367 hsa-miR-196a hsa-miR-452 hsa-miR-626 hsa-miR-369-5p
hsa-miR-196b hsa-miR-452 hsa-miR-548d hsa-miR-449 hsa-miR-205
hsa-miR-453 hsa-miR-639 hsa-miR-497 hsa-miR-208 hsa-miR-485-3p
hsa-miR-613 hsa-miR-501 hsa-miR-213 hsa-miR-488 hsa-miR-614
hsa-miR-509 hsa-miR-220 hsa-miR-490 hsa-miR-615 hsa-miR-510
hsa-miR-325 hsa-miR-492 hsa-miR-616 hsa-miR-511 hsa-miR-326
hsa-miR-493 hsa-miR-548c hsa-miR-514 hsa-miR-337 hsa-miR-503
hsa-miR-617 hsa-miR-515-3p hsa-miR-340 hsa-miR-504 hsa-miR-642
hsa-miR-515-5p hsa-miR-380-3p hsa-miR-505 hsa-miR-618 hsa-miR-517a
hsa-miR-422b hsa-miR-507 hsa-miR-644 hsa-miR-517b hsa-miR-422a
hsa-miR-513 hsa-miR-647 hsa-miR-517c hsa-miR-429 hsa-miR-516-5p
hsa-miR-649 hsa-miR-518a hsa-miR-491 hsa-miR-517 hsa-miR-661
hsa-miR-518b hsa-miR-496 hsa-miR-518c hsa-miR-662 hsa-miR-518c
hsa-miR-500 hsa-miR-518f hsa-miR-449b hsa-miR-518d hsa-miR-502
hsa-miR-519b hsa-miR-653 hsa-miR-518e hsa-miR-105 hsa-miR-519c
hsa-miR-411 hsa-miR-520a hsa-miR-122a hsa-miR-519d hsa-miR-654
hsa-miR-520b hsa-miR-124a hsa-miR-51ge hsa-miR-575 hsa-miR-520c
hsa-miR-126 hsa-miR-522 hsa-miR-576 hsa-miR-520d hsa-miR-128b
hsa-miR-523 hsa-miR-578 hsa-miR-520e hsa-miR-129 hsa-miR-524
hsa-miR-579 hsa-miR-520f hsa-miR-130a hsa-miR-526b hsa-miR-580
hsa-miR-520g hsa-miR-139 hsa-miR-96 hsa-miR-585 hsa-miR-520h
hsa-miR-143 hsa-miR-651 hsa-miR- 512-3p hsa-miR-95 hsa-miR-145
hsa-miR-376a hsa-miR-631 hsa-miR-126 hsa-miR-182 hsa-miR-542-5p
hsa-miR-363 hsa-miR-127 hsa-miR-185 hsa-miR-544 hsa-miR-487b
hsa-miR-133a hsa-miR-189 hsa-miR-656 hsa-miR-645 hsa-miR-135a
hsa-miR-18b hsa-miR-549 hsa-miR-556 hsa-miR-135b hsa-miR-202
hsa-miR-657 hsa-miR-558 hsa-miR-184 hsa-miR-202 hsa-miR-658
hsa-miR-627 hsa-miR-204 hsa-miR-299-3p hsa-miR-652 hsa-miR-630
hsa-miR-206 hsa-miR-302a hsa-miR-551a hsa-miR-603 hsa-miR-211
hsa-miR-302b hsa-miR-552 hsa-miR-606 hsa-miR-216 hsa-miR-302b
hsa-miR-553 hsa-miR-607 hsa-miR-217 hsa-miR-302c hsa-miR-554
hsa-miR-608 hsa-miR-330 hsa-miR-329 hsa-miR-555 hsa-miR-609
hsa-miR-371 hsa-miR-33 hsa-miR-562 hsa-miR-633 hsa-miR-379
hsa-miR-362 hsa-miR-563 hsa-miR-565* hsa-miR-381 hsa-miR-369-3p
hsa-miR-564 hsa-miR-594* miRNAs marked with * were not used in
analyses because they have been reclassified as non-miRNAs.
[0144] The B cell subsets were profiled for gene expression at the
whole genome level, as described previously. See Dave et al. N Engl
J Med. 2004; 351:2159-2169. At each stage, we identified
differentially expressed genes as those genes with a mean two-fold
difference in expression and a false-discovery rate of less than
1%. See FIGS. 1E, 1G, and 1I. Genes that we found to be
differentially expressed in each stage-transition were consistent
with previous studies that examined gene expression in B cell
subsets using microarrays with fewer probes, an overlap that was
found to be highly statistically significant (P<0.001,
chi-squared test). See, e.g., Klein et al. Proc Natl Acad Sci USA.
2003; 100:2639-2644; and Shaffer et al. Immunity. 2001; 15:
375-385.
[0145] In the naive.fwdarw.germinal center (GC) B cell transition,
we identified 32 miRNAs that were differentially expressed.
Interestingly, all but 4 miRNAs were found to be expressed more
highly in GC cells than in naive B cells. See FIG. 1D. We confirmed
the mRNA expression patterns of several genes that are known to be
differentially expressed in the transition including BCL6, MME,
MYBL1, as well as LM02. See FIG. 1E. LM02 was found to be expressed
more highly in germinal center B cells compared to both naive B
cells and memory B cells. See FIGS. 1E and 1I. In the
GC.fwdarw.plasma cell transition, we found 33 miRNAs that were
differentially expressed. Once again, we noted a striking
asymmetry, with all but 2 miRNAs found to be expressed highly in GC
cells, but down-regulated in plasma cells. See FIG. 1F. We also
confirmed that the plasma cell-specific genes, PRDM1 (FIG. 1G),
XBP1 and IRF4 were highly differentially expressed in our
experiments. In the GC.fwdarw.memory B cell transition, there was a
preponderance of the 27 significant miRNAs expressed at higher
levels in memory cells, See FIG. 1H. Five miRNAs were expressed
highly in GC cells compared to all the other B cell types. These
included 3 members of the miR-17.about.92 cluster (miR-17-5p,
miR-20b, miR-93), as well as miR-28 and miR-181b.
[0146] The expression pattern of all the miRNAs that were
measurable in at least one of the B cell subsets is summarized in
FIG. 5. Notably, there were no differences in the expression of
genes involved in miRNA processing, including DICER1, DROSHA, XP05
(exportin5), EIF2C2 (ag02) and DGCR8, among the B cell subsets. See
FIG. 1J.
[0147] Separately, we examined the expression of predicted target
genes of differentially expressed microRNAs. We also found that
predicted mRNA target genes of microRNAs expressed highly in GC
cells were expressed at lower levels in GC cells compared to other
stages. See FIG. 6 and Appendix A. FIG. 6A shows density plots of
the expression frequency of predicted mRNA targets of microRNAs
expressed highly in germinal center B cells compared to naive
cells. mRNAs from FIG. 1E that were also predicted targets of the
miRNAs (N=830) were plotted for both naive cells and germinal
center B cells. The depicted p-value was calculated using a 1-sided
Kolmogorov-Smirnov test. FIG. 6B shows density plots of the
expression frequency of predicted mRNA targets of miRNAs expressed
highly in germinal center B cells compared to plasma cells. mRNAs
from FIG. 1G that were also predicted targets of the miRNAs
(N=1098) were plotted for both plasma cells and germinal center B
cells. The depicted p-value was calculated using a 1-sided
Kolmogorov-Smirnov test. FIG. 6C shows density plots of the
expression frequency of predicted mRNA targets of miRNAs expressed
highly in the germinal center B cells compared to memory B cells.
mRNAs from FIG. 1I that were also predicted targets of the miRNAs
(N=269) were plotted for both naive cells and germinal center B
cells. The depicted p-value was calculated using a 1-sided
Kolmogorov-Smirnov test.
[0148] In the naive to germinal center transition (see FIG. 6A),
genes with at least a two-fold change in expression were analyzed
by SAM using a false discovery rate of less than 1% (Larsson et.
al, BMC Bioinformatics. 2005; 6:129), depicted in FIG. 1E. From
that list of significantly differentially expressed genes, we
determined those that were predicted targets of miRNAs (defined as
genes with 3'UTR sequence complementarity to microRNA nucleotides
2-7) that were expressed more highly in germinal center cells. The
gene expression distribution of those mRNA targets was graphed for
naive B Cells (blue curve) and germinal center B cells (orange
curve). Thus, in FIG. 6A, we show genes differentially expressed
between naive and germinal center B cells that are also predicted
targets of miRNA expressed more highly in germinal center B cells.
We observe that the expression of these microRNA target genes is
lower in germinal center B cells compared to naive B cells.
[0149] A similar analysis was carried out for the transitions from
germinal center to plasma cell (FIG. 6B) and germinal center to
memory cell (FIG. 6C). In all three cases examined, the
distribution of germinal center miRNA target gene expression is
statistically significantly lower (leftward shift of orange curve),
which suggests a possible gene regulatory role for the miRNAs that
are more highly expressed in germinal center cells. We note that
the observed lower expression of the miRNA target genes could,
however, also be caused by other factors such as downstream effects
of particular transcription factors. The complete set of genes
plotted in FIG. 6 is listed in Appendix A.
[0150] Finally, we found that a higher proportion of differentially
expressed transcription factors are predicted microRNA targets. See
FIG. 7. Table 6 shows differentially expressed transcription
factors, with the average level of expression for each in naive,
germinal center, plasma, and memory cells. Transcription factors
for which the significance analysis showed a q value of 0 are
indicated.
TABLE-US-00006 TABLE 6 Differentially-expressed transcription
factors Naive GC Germinal Plasma vs GC vs Naive Center Cell Memory
GC: q vs PC: q Mem: q ID NAME average average average average value
value value 1 AHR 8.9 10.33 8.36 10.38 0 0 2 APBB2 6.86 7.87 7.35
6.39 0 0 3 ARID1A 12.3 12.22 11.11 12.17 0 4 ARID3A 8.03 6.9 9.81
8.76 0 0 0 5 ARNTL 9.47 8.4 8.23 9.7 0 0 6 ATF7 7.83 8.97 8.23 8.44
0 7 BACH1 10.68 9.97 11.76 10.59 0 8 BHLHB3 11.11 8.05 13.26 13.26
0 0 0 9 BPTF 12.23 12.46 11.34 11.86 0 10 BTG1 15.88 14.95 13.94
15.28 0 11 CASP8AP2 10.17 10.55 9.44 10.22 0 12 CBL 11.07 11.69
10.26 11.04 0 13 CBX4 12.23 12.36 13.69 12.68 0 14 CCNE1 7.86 9.18
8.87 7.66 0 0 15 CDH1 5.85 3.85 10.26 4.91 0 16 CITED2 9.11 9.88
12.56 9.22 0 17 CLOCK 9.85 7.68 9.91 9.62 0 0 0 18 CREB5 4.91 5.46
7.24 6.95 0 19 CREBL1 7.53 6.97 8.06 8.09 0 20 CREBL2 10.18 9.95
11.1 10.79 0 21 CSDA 12.12 8.59 10.1 8.98 0 0 22 DEK 12.73 14.09
10.29 13.15 0 0 23 DLX2 6.08 4.66 7.04 7.08 0 0 24 DR1 11.83 11.97
10.65 12.1 0 25 DTX1 10.98 11.6 10.55 9.62 0 0 26 DYRK1B 6.32 5.92
7.61 4.96 0 27 E2F1 7.01 8.37 7.95 7.21 0 0 28 EGR1 11.92 13.24
13.77 12.75 0 29 EGR2 10.21 10.43 8.07 10.4 0 30 EGR3 11.59 9.35
8.24 10.62 0 0 0 31 ELF1 12.39 12.7 11.34 12.55 0 32 ELL 7.12 7.83
9.17 7.59 0 33 ELL2 9.25 8.23 13.6 9.27 0 34 EPAS1 7.1 7.02 8.39
7.5 0 35 ETS1 14.25 14.4 12.62 14.17 0 36 ETS2 6.37 7.5 5.66 4.9 0
0 0 37 ETV1 8.19 6.9 7.38 7.06 0 38 ETV4 5.99 5.42 7.15 6.09 0 39
ETV6 10.36 7.14 8.89 10.43 0 0 0 40 FHL2 4.82 7.77 6.82 5.65 0 0 41
FLNA 9.95 9.2 8.57 11.03 0 42 FOS 12.35 11.25 13.34 11.23 0 0 43
FOSB 10.13 7.21 11.05 10.79 0 0 0 44 FOXC1 6.81 5.84 7.39 6.59 0 45
FOXF2 7.05 4.83 6.37 5.84 0 46 FOXJ2 10.01 8.38 9.97 9.85 0 0 0 47
FOXK2 8.94 8.4 9.63 8.71 0 48 GATA3 8.05 7.76 8.51 9.6 0 49 GATA6
7.08 5.41 7.59 6.26 0 0 50 GCN5L2 10.18 8.97 8.9 10.36 0 0 51 GLI2
6.93 7.26 7.52 6.09 0 52 GPX3 6.72 5.92 7.61 5.58 0 53 HCLS1 13.94
14.42 13.12 13.92 0 54 HIPK2 9.36 8.66 11.04 10.85 0 0 55 HMGA1
11.26 12.47 10.36 10.86 0 0 0 56 HMGB1 15.47 16.36 14.98 14.97 0 0
57 HMGB2 12.96 15.78 12.73 13.36 0 0 0 58 HOXA1 5.36 7.7 7.23 7.64
0 59 HOXA5 7.03 8.32 8.36 7.54 0 60 HOXB4 8.2 7.58 9.27 8.01 0 61
HOXB9 7.4 6.2 8.41 6.87 0 62 HOXC11 6.6 6.4 7.88 6.21 0 63 HOXC8
7.05 5.48 6.88 6.71 0 64 ID4 6.49 5.93 7.79 6.41 0 65 ILF3 11.26
11.94 10.71 10.99 0 66 IRAK1 12.19 11.48 12.8 11.96 0 67 IRF4 11.16
9.28 13.66 10.51 0 0 0 68 ISL1 6.98 5.91 7.6 6.11 0 69 JAZF1 11.93
10.65 7.49 11.31 0 0 70 JMJD1C 12.34 12.34 11.11 12.56 0 71 JUN
12.14 9.34 12.7 12.16 0 0 0 72 JUNB 11.62 9.72 10.84 11.37 0 0 73
KLF11 8.08 5.94 6.88 8.39 0 0 74 KLF2 12.61 9.1 11.7 12.61 0 0 0 75
KLF4 10.04 7.57 9.09 7.49 0 0 76 KLF5 7.08 5.26 6.14 5.07 0 77 KLF6
12.06 11.33 9.24 12.40 0 0 78 KLF7 9.36 8.35 8.88 9.8 0 79 KLF9
9.47 7.34 6.64 9.27 0 0 80 LITAF 12.52 10.18 11.82 12.49 0 0 0 81
LMO1 6.86 4.75 7.16 6.88 0 82 MAF 8.05 9.95 8.25 10.83 0 0 83 MAFB
6.58 7.3 8.56 7.29 0 84 MAML3 8.05 10.85 9.07 7.04 0 0 0 85 MDFIC
12.06 13.67 13.6 13.08 0 86 MEIS2 7.45 7.15 7.56 5.29 0 87 MEN1
10.79 11.33 10.49 10.23 0 88 MITF 7.51 8.05 8.01 6.41 0 89 MTF1
10.08 9.92 8.86 10.5 0 90 MXD1 9.07 7.59 9.62 8.9 0 0 0 91 MXI1
10.63 7.85 11.1 9.42 0 0 0 92 MYB 6.97 8.62 5.61 7.99 0 0 93 MYBL1
9.68 14.13 10.02 9.72 0 0 0 94 MYBL2 9.07 12.41 9.12 8.52 0 0 0 95
NAT14 6.62 7.66 7.33 7.63 0 96 NF1 9.23 10.46 11.21 9.88 0 97 NFAT5
11.71 12.09 10.72 11.77 0 98 NFATC4 6.36 7.92 8.96 5.71 0 0 99 NFIX
8.09 7.43 8.67 7.37 0 100 NFKB1 11.91 11.9 10.7 11.55 0 101 NFYC
10.2 10.27 9.03 10.03 0 102 NR3C2 8.85 5.58 4.91 8.41 0 0 103 NR6A1
9.1 6.03 6.42 7.62 0 0 104 NRIP1 11.79 10.3 10.33 11.20 0 105 PAX3
7.01 5.55 5.01 5.96 0 106 PHF1 11.85 10.69 12.26 11.45 0 0 107
POU3F1 6.08 7.52 6.86 7.52 0 108 POU4F1 5.62 8.85 8.46 7.53 0 0 109
POU4F2 7.09 5.72 7.21 5.1 0 0 110 PPARD 11.23 9.98 10.29 10.69 0
111 PPARG 7.79 7.22 6.74 5.47 0 112 PPARGC1B 6.89 6.09 6.51 7.91 0
113 PRDM1 8.49 9.15 14.1 9.33 0 114 PRDM4 10.48 9.81 9.76 10.83 0
115 PROX1 7.18 6.38 7.89 5.54 0 116 RAN 13.46 14.57 12.39 13.78 0 0
117 RSF1 10.05 9.1 9.56 10.18 0 118 RUNX1T1 7.37 5.95 6.63 6.34 0
119 RUNX2 8.75 7.91 10.22 9.75 0 0 120 RUNX3 11.8 11.13 10.26 12.47
0 121 RXRA 9.07 7.15 8.81 8.32 0 0 0 122 SAP30 8.22 9.91 9.28 8.33
0 0 123 SCMH1 8.48 9.69 9.65 8.62 0 0 124 SCML1 10.32 8.48 7.81
7.91 0 125 SCML2 10.33 8.76 9.27 8.96 0 126 SF1 11.72 11.44 10.36
11.37 0 127 SIAH2 10.35 12.34 10.57 9.90 0 0 0 128 SLC2A4RG 8.25
8.98 7.4 8.91 0 129 SMAD1 5.91 8.1 6.34 7.33 0 0 130 SMAD2 10.45
11.57 10.8 10.98 0 131 SMAD3 10.72 9.23 7.74 10.54 0 0 0 132
SMARCA2 11.14 10.12 10.79 11.19 0 0 133 SOLH 8.78 7.88 8.7 9.03 0
134 SOX4 8.99 9.14 9.17 10.2 0 135 SOX5 9.3 10.47 7.33 9.08 0 0 0
136 SOX9 6.72 8.42 8.18 7.03 0 0 137 SP4 11.26 10.14 9.58 11.75 0 0
138 SRCAP 8.58 8.06 6.96 8.38 0 139 SREBF1 8.23 8.36 6.56 5.89 0 0
140 STAT5B 10.35 10.95 9.73 10.7 0 141 SUFU 7.12 6.03 7.84 7.62 0 0
142 SUPT16H 11.12 11.35 9.6 10.71 0 143 SUPT3H 7.77 6.3 8.05 8.35 0
0 0 144 TARDBP 12.31 12.72 11.49 11.91 0 145 TBP 10.55 10.14 9.09
10.38 0 146 TBX3 5.67 6.05 7.89 5.53 0 147 TCEA1 13.31 15 14.05
13.6 0 0 148 TCERG1 12.29 12.5 11.46 12.04 0 149 TCF7 10.21 10.82
9.97 12.61 0 150 TFAP2A 6.7 5.54 8.26 6.88 0 151 TFAP4 7.28 5.29
5.42 5.13 0 152 TFDP1 10.95 12.9 10.49 11.36 0 0 0 153 TFEB 11.56
11.15 9.49 11.77 0 154 THRA 7.41 7.26 8.48 7.05 0 155 THRB 7.45
7.51 6.11 6.29 0 0 156 TLE1 9.19 7.74 9.63 9.13 0 0 0 157 TMF1
10.76 9.68 10.71 10.42 0 158 TSC22D3 13.22 11.26 12.67 13.4 0 0 0
159 UHRF1 9.23 12.22 8.72 8.96 0 0 0 160 VEZF1 10.98 11.45 9.94
10.81 0 161 XBP1 10.1 10.74 15.84 10.65 0 162 YBX1 14.79 15.31 14.2
14.53 0 163 YWHAH 8.94 10.82 9.09 9.36 0 0 0 164 YWHAZ 13.38 13.62
12.61 13.62 0 165 ZFP36L1 14.07 12.89 10.09 13.87 0 0 166 ZHX3 8.29
7.76 7.47 6.66 0 167 ZNF207 12.29 13.51 12.69 12.54 0 168 ZNF217
11.68 10.77 9.73 11.61 0 169 ZNF219 6.13 6.11 8.42 6.48 0 170
ZNF238 13.05 11.27 10.08 12.54 0 0 0 171 ZNF3 8.86 7.27 8.75 8.89 0
0 0 172 ZNF367 7.97 10.99 8.04 8.22 0 0 0 173 ZNF398 10.39 9.28
10.47 10.25 0 0
Example 3
MiR-223 Regulates LM02 in the Naive.about.Germinal Center and
Germinal Center.about.Memory Cell Transitions
[0151] MiR-223 has previously been described as being important in
the commitment to myeloid lineage. See Chen et al. Science. 2004;
303:83-86; Johnnidis et al. Nature. 2008; 451:1125-1129. We found
miR-223 to be expressed at nearly 8-fold higher levels in both
naive and memory cells compared to GC cells. This miRNA has a
highly conserved sequence complementarity to the 3 'UTR of 2
transcription factors that are expressed highly during GC cell
differentiation: LM02 (FIG. 2A) and MYBL1 (FIG. 8A). Higher
expression of miR-223 in the naive B cell stage could inhibit the
untimely expression of these transcription factors until the cell
is ready to undergo the germinal center reaction.
[0152] We evaluated the effects of miR-223 expression on its
predicted target gene, LM02, by transfecting precursors of miR-223
into a cell line derived from GC cell lymphoma cells (B1AB).
Over-expression of miR-223 resulted in a consistent down-regulation
of LM02 at the transcript level compared to a transfection with a
scrambled control with no sequence complementarity to the human
genome. See FIG. 2B. In that figure, the blue bars depict
expression of LM02 24 hours after transfection with a scrambled
control that does not possess complementarity to the human genome.
The orange bars depict the expression of LM02 24 hours after
transfection with a precursor for miR-223. The expression of LM02
was consistently lower in the cells treated with the miR-223
precursor, and the results were statistically significant
(P<0.05 in all cases, student's t-test). There was no effect on
the expression of a non-target control, beta-2 micro globulin in
these experiments (data not shown). Over-expression of miR-223 also
resulted in a consistent down-regulation of LM02 at the protein
level compared to a transfection with a scrambled control with no
sequence complementarity to the human genome. See FIG. 2C. We
quantified the results of 3 separate experiments examining LM02
protein expression and found consistent down-regulation of LM02 in
cells treated with miR-223 compared to cells transfected with
scrambled controls. See FIG. 2D. Those results that were
statistically significant (P<0.05, student's t-test). The extent
of down-regulation of LM02 mRNA and protein by miR-223 was
comparable, suggesting that miR-223 regulation of LM02 occurs
predominantly at the mRNA level. Similarly, over-expression of
miR-223 resulted in a down-regulation of MYBL1 transcripts. See
FIG. 8B. In that figure, the blue bars depict expression of MYBL1
mRNA 24 hours after transfection with a scrambled control that does
not possess complementarity to the human genome. The orange bars
depict the expression of Mybl1 24 hours after transfection with a
precursor for miR-223. The expression of LM02 was consistently
lower in the cells treated with the miR-223 precursor (P<0.05 in
all cases).
[0153] As additional validation, we investigated whether the
miR-223 had a direct effect on LM02 by cloning the 3'UTR sequence
of LM02 3' to the firefly luciferase ORF (Fluc). See Gottwein et
al. Nature. 2007; 450:1096-1099. The resulting constructs and the
unmodified vector were co-transfected into 293T cells along with a
Renilla luciferase internal control and pL-CMV-eGFP constructs
expressing either no miRNA or miR-223. Fluc expression from
constructs bearing LM02 3' UTR sequences were differentially
down-regulated by miR-223 compared to those with mutated seed
sequences; the seed sequence mutant construct had consistently
diminished miR-223 repression compared to the wild-type construct
in 5 separate experiments. See FIG. 2E. Those results were
statistically significant (P<0.05, student's t-test). These
observations provide evidence for an inhibitory role for miR-223 in
regulating the transcription factor LM02.
[0154] Activation of LM02 has been associated with the development
of leukemia in patients undergoing gene therapy. Hacein-Bey-Abina
et al. Science. 2003; 302:415-419. On the other hand, higher
expression of PRDM1 alone is sufficient to induce plasma
cell-differentiation. Turner et al. Cell. 1994; 77:297-306.
Inappropriate expression of such genes must be effectively turned
off for a cell to maintain its state. This mode of regulation is
reflected in the effects of miR-223, miR-9 and miR-30, which turn
off the inappropriate expression of LM02 and PRDM1 and might
promote state maintenance and inhibition of lymphomagenesis.
[0155] On the other hand, our data also identify a number of
instances in which miRNAs are co-expressed with their predicted
targets. It is possible that such interactions within the cell help
to stabilize a defined expression level by dampening fluctuations.
For example, in GC cells, we found that miR-181b was strongly
co-expressed with its predicted target, BCL6. Such interactions
could also be important in B cell stage maintenance and curbing the
oncogenic potential of genes involved in B cell differentiation.
See Cattoretti et al. Cancer Cell. 2005; 7:445-455; Dorsett et al.
Immunity. 2008; 28:630-638.
Example 4
MiR-9 and the miR-30 Family Regulate PRDM1 (blimp-1) in the
Germinal Center.fwdarw.Plasma Cell Transition
[0156] In the GC.fwdarw.plasma cell transition, we observed that
several members of the miR-30 family were expressed at 2-fold or
higher levels in GC cells. See FIG. 1F. The transcription factor
PRDM1 is an essential regulator of plasma cell differentiation.
Martins et al. Annu Rev Immunol. 2008; 26:133-169. The miR-30
family comprises 5 members (miR-30a, 30b, 30c, 30d and 30e), of
which 4 (all except 30e) were found to be expressed at higher
levels in GC cells compared to plasma cells. Control transfection
experiments documented good specificity of the RT-PCR probes for
individual members of the miR-30 family with no discernible
cross-hybridization. See FIG. 9. Three separate transfection
experiments using high concentrations of miR-30b precursors are
shown in the left panel with measurement of miR-30b and miR-30d.
Similar experiments were performed with precursors for miR-30d
(right panel).
[0157] The 3'UTR of PRDM1 contains 3 highly conserved binding sites
complementary to the seed sequence of members of the miR-30 family,
as well as 3 binding sites for the seed sequence of miR-9, 2 of
which are highly conserved across multiple species. See FIG. 3A.
The 3'UTR region of PRDM1 complementary to miR-30 is shown in red.
The 3 sites are complementary to nucleotides 2-8 (UTR position
408), nucleotides 1-8 (UTR position 2370) and nucleotides 2-8 (UTR
position 2383) on the miRNA, respectively. The 3'UTR region of
PRDM1 complementary to miR-9 is shown in green. The 3 sites are
complementary to nucleotides 1-7 (UTR position 1459), nucleotides
2-8 (UTR position 2108) and nucleotides 2-8 (UTR position 2323) on
the miRNA respectively. These sites are highly conserved across a
number of species, with the exception of one miR-9 site (UTR
position 1459) that is only present in humans.
[0158] To evaluate the effects of the miR-30 family and miR-9 on
PRDM1 expression in plasma cells, we introduced precursors of
miR-9, miR-30b and miR-30d into the U266 multiple myeloma (plasma
cell) cell line. Overexpression of miR-30 family members miR-30b
and miR-30d, as well as miR-9, had no effect on PRDM1 at the mRNA
level. See FIG. 3B. In that figure, the blue bars depict expression
of PRDM1 24 hours after transfection with a scrambled control with
no complementarity to the human genome. The magenta bars depict the
expression of PRDM1 24 hours after transfection with a hairpin
precursor for miR-30b, while the red bars depict the expression of
PRDM1 24 hours after transfection with a hairpin precursor for
miR-30d. The green bars depict the expression of PRDM1 24 hours
after transfection with a hairpin precursor for miR-9. By contrast,
there was a consistent down-regulation of PRDM1 at the protein
level. See FIGS. 3C and 3D. Those results were statistically
significant in each case (P<0.05, student's t-test), except for
the transfections with the precursor to miR-9 (P=0.08, student's
t-test). Overexpression of each of those microRNAs had an average
knockdown effect of around 40%. These exclusively
post-transcriptional effects of miR-9 and miR-30 on PRDM1
expression are consistent with one mechanism of miRNA regulation
that has been described previously in other systems. See, e.g.,
O'Donnell et al. Nature. 2005; 435:839-843; Gottwein et al. Nature.
2007; 450:1096-1099; Selbach et al. Nature. 2008; 455:58-63; and
Back et al. Nature. 2008; 455:64-71. There was no effect on the
expression of a non-target control (Actin).
[0159] Additionally, luciferase reporter activity of the PRDM1
3'UTR construct was decreased by overexpression of miR-9, miR-30b,
and miR-30d, but not their respective seed sequence mutants. See
FIG. 3E, which shows the average of three experiments. The
down-regulation of the luciferase reporter signal and its
restoration in the mutant constructs was found to be statistically
significant in each of the 3 microRNAs: miR-9, miR-30b and miR-30d
(P<0.05, student's t-test). The luciferase reporter activity
level was rescued to the activity level of the empty vector control
when the seed sequence of the microRNAs was mutated.
[0160] The combined effect of 5 different microRNA species
(miR-30a, miR-30b, miR-30c, miR-30d and miR-9) is likely to be more
potent than that of a single microRNA. The role of mutual
repression of BCL6 and PRDM1 in the germinal center to plasma cell
differentiation as been described previously. See Martins et al.
Immunol. 2008; 26:133-169. Our data suggest that microRNAs may
bolster the effects of BCL6 in the inhibition of PRDM1.
[0161] Our data show that members of the miR-17.about.92 family are
consistently expressed in GC cells and may play a role in mature B
cell differentiation. Interestingly, the miR-17.about.92 family has
been implicated in early B cell differentiation and mice lacking
the loci that encode these miRNAs have arrested early B cell
development. See Ventura et al. Cell. 2008; 132:875-886. The
expression patterns of the miR-17.about.92 family suggest that the
regulatory motifs embedded in the interaction of this miRNA family
and its targets might have an additional function in regulating
mature B cell differentiation.
[0162] A striking observation in this study is the high degree of
asymmetry observed in relative expression of miRNAs in GC cells
compared to naive and plasma cells. At least 2 hypotheses could
account for these findings. First, miRNA expression may promote a
highly regulated state that enables GC cells to interact with T
cells and antigen presenting cells, and to leave the GC cells
poised for differentiation into memory or plasma cells. Second,
miRNAs expressed highly in naive and plasma cells may be
underrepresented in current miRNA libraries. Such libraries are
often constructed from lymph nodes, which are typically enriched in
GC cells. High throughput sequencing of sorted populations of B
cells could reveal novel miRNAs that are highly expressed in those
populations. Interestingly, a larger number of miRNAs were highly
expressed in memory cells compared to GC cells. This observation
might stem from the fact that memory cells are known to be
heterogeneous (Sanz et al. Semin Immunol. 2008; 20:67-82) and
standard methods used to select memory cells may capture a diverse
group of memory subpopulations.
Example 5
MiRNAs and B Cell Malignancies
[0163] To examine the expression of B cell stage-specific microRNAs
in B cell malignancies, we undertook miRNA profiling of 75 tissue
samples derived from normal lymph nodes (N=5) as well as patients
with B cell malignancies including the molecular subsets of diffuse
large B cell lymphoma (DLBCL; see Rosenwald et al. N Engl J Med.
2002; 346:1937-1947), germinal center B cell-like (GCB) DLBCL
(N=20) and activated B cell-like (ABC) DLBCL (N=20), as well as
cases of IgV mutated and unmutated chronic lymphocytic leukemia
(N=20) and Burkitt lymphoma (N=10).
[0164] We constructed a Bayesian predictor from miRNAs that
distinguished normal memory B cells from GC cells. See FIG. 1H. We
tested the predictor in the B cell malignancies derived from
germinal center B cells (Burkitt lymphoma and GCB DLBCL) along with
chronic lymphocytic leukemia, which is thought to arise from memory
B cells. See Klein et al. J Exp Med. 2001; 194:1625-1638. Using
leave one out cross-validation, we found that the predictor
constructed from miRNAs expressed in normal B cells was able to
correctly identify the B cell specific stage of the B cell
malignancy type in over 95% of the cases. See FIG. 4A.
[0165] An interesting aspect regarding the role of miRNAs in
malignancies is their reported down-regulation in a number of
malignancies compared to normal cells from the same lineage. See Lu
et al. Nature. 2005; 435:834-838. To further examine this effect in
B cell malignancies, we examined the expression of 113 miRNAs that
we had identified in normal B cells. See Table 4, above. Of the 113
miRNAs, 103 were detected using the microarrays that we used to
profile B cell malignancies and normal lymph nodes. We applied a
2-sided student's t-test to evaluate the relative expression of
those 103 miRNAs in B cell malignancies (N=60) and normal lymph
nodes (N=5). 34 miRNAs were differentially expressed (P<0.05) at
higher levels in malignant cells and 9 miRNAs were expressed more
highly in normal cells. 60 miRNAs were not differentially
expressed. See FIG. 4B.
[0166] As additional validation, we examined miRNA cloning
frequencies for sequences cloned from normal and malignant B cells.
See Landgraf et al. Cell. 2007; 129:1401-1414. MiRNAs for which a
sequence was identified in at least 2 of the 3 available normal B
cell samples were used in the analysis. We applied a 2-sided
student's t-test (P<0.05) to compare the differential cloning
frequency of the miRNAs between normal B cells (N=3) and a variety
of mature B cell malignancy patient samples and cell lines (N=42).
In all, we found 56 miRNAs that were consistently expressed in
normal B cells. We found 13 of those 56 miRNAs were differentially
expressed (P<0.05) between normal and malignant B cells, of
which 12 miRNAs were expressed more highly in malignant cells and 1
miRNA was expressed more highly in normal cells. See FIG. 4C. In
order to avoid effects from tumor-infiltrating non-malignant cells,
we repeated the analysis with 20 chronic lymphocytic leukemia
samples in the malignant group. The results were similar to those
obtained with the larger set of malignancies (data not shown).
[0167] These results demonstrate that miRNAs are not down-regulated
in B cell malignancies compared to normal B cells and that normal B
cell stage-specific miRNAs are maintained in B cell
malignancies.
[0168] MicroRNA profiling also revealed that each B cell malignancy
type had a distinctive pattern of miRNA expression. See FIG. 4D. In
order to evaluate the ability of miRNA profiles to distinguish
different B cell malignancy types, we constructed Bayesian
predictors from the most highly differentially expressed miRNAs for
each pair-wise comparison. See Tables 7, 8, and 9.
TABLE-US-00007 TABLE 7 Predictor microRNAs that distinguish
germinal center B-cell (GCB) DLBCL from Burkitt Lymphoma, activated
B-cell (ABC) DLBCL, and chronic lymphocytic leukemia GCBvsBL
GCBvsABC GCBvsCLL hsa-miR-146a hsa-miR-142-3p hsa-miR-126
hsa-miR-154 hsa-miR-16 hsa-miR-130a hsa-miR-155 hsa-miR-184
hsa-miR-I0b hsa-miR-184 hsa-miR-191 hsa-miR-154 hsa-miR-29b
hsa-miR-19a hsa-miR-193b hsa-miR-29c hsa-miR-19b hsa-miR-199a-3p
hsa-miR-363 hsa-miR-299-5p hsa-miR-365 hsa-miR-503 hsa-miR-32
hsa-miR-99b hsa-miR-519c-5p hsa-miR-30e* hsa-miR-143 hsa-miR-301a
hsa-miR-151-5p hsa-miR-585 hsa-miR-152 hsa-miR-583 hsa-miR-193a-5p
hsa-miR-30b* mghv-miR-MI-7-5p hsa-miR-886-5p hsa-miR-590-5p
hsa-miR-142-5p hsa-miR-I00 hsa-miR-149* hsa-miR-106b hsa-miR-768-5p
hsa-miR-300 hsa-miR-30e hsa-miR-145 hsa-miR-625* hsa-miR-140-3p
hsa-miR-943 kshv-miR-K12-3 hsa-miR-20a hsa-miR-371-5p hsa-miR-28-5p
hsa-miR-526b* hsa-miR-675 hsa-miR-25* hsa-miR-28-5p hsa-miR-150
ebv-miR-BHRFI-2 hsa-miR-30c hsa-miR-181a BL = Burkitt lymphoma, ABC
= activated B-cell DLBCL, CLL = chronic lymphocytic lymphoma
TABLE-US-00008 TABLE 8 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from Burkitt Lymphoma and chronic
lymphocytic leukemia ABCvsBL ABCvsCLL hsa-miR-182 hsa-miR-193b
hsa-miR-377 hsa-miR-99b hsa-miR-660 hsa-miR-551a hsa-miR-595
mghv-miR-MI-7-3p ebv-miR-BARTI0 hsa-miR-585 hsa-miR-532-5p
hsa-miR-617 hsa-miR-200c* hsa-miR-629* hsa-miR-362-3p hsa-miR-575
hsa-miR-455-3p kshv-miR-K12-6-5p hsa-miR-128 hsa-miR-193a-5p
hsa-miR-21* hsa-miR-30e hsa-miR-589 hsa-miR-296-3p hsa-miR-135a*
hsa-miR-518b hsa-miR-532-3p hsa-miR-492 hsa-miR-548d-5p
hsa-miR-220c hsa-miR-652 hsa-miR-326 hsa-miR-150* hsa-miR-671-5p
hsa-miR-330-5p hsa-miR-340* hsa-miR-339-3p hsa-miR-150
hsa-miR-502-3p hsa-miR-193b* BL = Burkitt lymphoma, CLL = chronic
lymphocytic lymphoma.
TABLE-US-00009 TABLE 9 Predictor microRNAs that distinguish Burkitt
lymphoma from chronic lymphocytic leukemia BLvsCLL hsa-miR-130b
hsa-miR-154 hsa-miR-155 hsa-miR-29b hsa-miR-29c hsa-miR-637
hsa-miR-658 hsa-miR-193a-5p hsa-miR-886-5p hsa-miR-768-5p
hsa-miR-101 hsa-miR-933 hsa-miR-371-5p hsa-miR-675 hsa-miR-150
hsa-miR-874 hsa-miR-181a hsa-miR-30c ebv-miR-BHRFI-2 hsa-miR-628-3p
CLL = chronic lymphocytic lymphoma
[0169] We tested the performance of the predictor using
leave-one-out cross-validation applied to the predictors for
Burkitt lymphoma, chronic lymphocytic leukemia, activated B-cell
diffuse large B-cell lymphoma, and germinal center B-cell DLBCL.
For a sample prediction to be correct, it had to be classified
correctly in each pair-wise comparison with all remaining entities.
We found it to be over 90% accurate in the identification of each
entity. See FIG. 10.
[0170] We next expanded the analysis to include follicular lymphoma
and Hodgkin's lymphoma. We included the samples from the earlier
study and we undertook miRNA profiling of the following additional
samples: normal lymph nodes (N=5) and patients with germinal center
B cell-like (GCB) DLBCL (N=10), activated B cell-like (ABC) DLBCL
(N=10), chronic lymphocytic leukemia (N=10), Burkitt lymphoma
(N=10), follicular lymphoma (N=10), and Hodgkin's lymphoma (N=9).
As before, microRNA profiling revealed that each B cell malignancy
type had a distinctive pattern of miRNA expression. See FIG.
11.
[0171] From that data, we identified microRNAs that can be used to
distinguish each of the six B cell malignancies analyzed from the
other five. Lists of those microRNAs, and whether they are
expressed in high levels or low levels relative to the other 5 B
cell malignancies, are shown in Tables 10 through 13. In certain
embodiments, each of those microRNAs could be used to distinguish
one of the six B cell malignancies from the other five. High and
low in the table refer to at least a 2 fold difference when
compared to other lymphomas and benign lymph nodes.
TABLE-US-00010 TABLE 10 Predictor microRNAs that distinguish
Burkitt lymphoma, germinal center B-cell (GCB) DLBCL, and
follicular lymphoma from all other B cell malignancies in the study
BL vs GCB vs FL vs BL miRNA list others GCB miRNA list others FL
miRNA list others hsa-miR-106a High hsa-miR-93/mmu-miR- High
hsa-miR-96/mmu-miR- High hsa-miR-17/mmu- High 93/rno-miR-93
96/rno-miR-96 miR-17/rno-miR-17- hsa-miR-886-3p High
hsa-miR-138/mmu-miR- High 5p/rno-miR-17 hsa-miR-365/mmu-miR- High
138/rno-miR-138 hsa-miR-628-3p High 365/rno-miR-365
hsa-miR-342-5p/mmu- High hsa-miR-193a-5p High hsa-miR-378/mmu-miR-
High miR-342-5p/rno-miR-342- hsa-miR-371-5p High 378/rno-miR-378 5p
hsa-miR-20a/mmu- High hsa-miR-320/mmu-miR- High hsa-miR-337-3p High
miR-20a/rno-miR-20a 320/rno-miR-320 hsa-miR-301a/mmu-miR- High
hsa-miR-23a/mmu- Low hsa-miR-103/mmu-miR- High 301a/rno-miR-301a
miR-23a/rno-miR-23a 103/rno-miR-103 hsa-miR-922 High
hsa-miR-27a/mmu- Low hsa-miR-637 High hsa-miR-744/mmu-miR- High
miR-27a/rno-miR-27a hsa-miR-148a/mmu- High 744 hsa-miR-34b/mmu- Low
miR-148a hsa-miR-221* High miR-34b-3p hsa-miR-199a-3p/hsa- High
hsa-miR-215 High hsa-miR-21/mmu- Low miR-199b-3p/mmu-miR-
hsa-miR-197/mmu-miR- High miR-21/rno-miR-21 199a-3p/mmu-miR- 197
hsa-miR-29a/mmu- Low 199b/rno-miR-199a-3p hsa-miR-92b/mmu-miR- High
miR-29a/rno-miR-29a hsa-miR-126*/mmu- High 92b/rno-miR-92b
hsa-let-7b/mmu-let- Low miR-126-5p/rno-miR- hsa-miR-218-2*/mmu-
High 7b/rno-let-7b 126* miR-218-2*/rno-miR- hsa-let-7f/mmu-let- Low
kshv-miR-K12-6-3p High 218* 7f/rno-let-7f hsa-miR-125a-5p/mmu- High
hsa-miR-636 High hsa-miR-29b/mmu- Low miR-125a-5p/rno-miR-
hsa-miR-600 High miR-29b/rno-miR- 125a-5p kshv-miR-K12-7 High 29b
hsa-miR-551b/mmu- Low hsa-miR-194/mmu-miR- High hsa-miR-549 Low
miR-551b/rno-miR-551b 194/rno-miR-194 hsa-miR-374a Low hsa-miR-923
Low hsa-miR-524-5p High hsa-miR-513a-5p Low hsa-miR-22*/mmu-miR-
High hsa-miR-148a/mmu- Low 22*/rno-miR-22* miR-148a
hsa-miR-34c-5p/mmu- High hsa-miR-223/mmu- Low miR-34c/rno-miR-34c
miR-223/rno-miR-223 hsa-miR-151-3p High hsa-miR-138- Low
hsa-miR-425/mmu-miR- High 1*/mmu-miR- 425/rno-miR-425
138*/rno-miR-138* ebv-miR-BART16 High hsa-miR-342- Low hsa-miR-20b*
High 3p/mmu-miR-342- hsa-miR-574-3p/mmu- High 3p/rno-miR-342-3p
miR-574-3p hsa-miR-146b- Low hsa-miR-155* High 5p/mmu-miR-
hsa-miR-374b* High 146b/rno-miR-146b hsa-miR-497/mmu-miR- High
hsa-miR-195/mmu- Low 497/rno-miR-497 miR-195/rno-miR-195
hsa-miR-708/mmu-miR- High hsa-miR-103/mmu- Low 708/rno-miR-708
miR-103/rno-miR-103 hsa-miR-92a/mmu-miR- High hsa-miR-888* Low
92a/rno-miR-92a hsa-miR-363/mmu- Low hsa-miR-361-3p High
miR-363/rno-miR-363 hsa-miR-513a-3p High hsa-miR-140- Low
hsa-miR-934 High 3p/mmu-miR- hsa-miR-363*/rno-miR- High
140*/rno-miR-140* 363* hsa-miR-191/mmu- Low mghv-miR-M1-3 High
miR-191/rno-miR-191 hsa-miR-129* High hsa-miR-768-5p Low
hsa-miR-148b/mmu-miR- High hsa-miR-222/mmu- Low
148b/rno-miR-148b-3p miR-222/rno-miR-222 hsa-miR-493 High
hsa-miR-668/mmu- Low hsa-miR-151-5p/mmu- High miR-668
miR-151-5p/rno-miR-151 hsa-miR-221/mmu- Low hsa-miR-146b-3p High
miR-221/rno-miR-221 hsa-miR-886-5p High hsa-miR-24-1*/mmu- Low
hsa-miR-331-3p/mmu- High miR-24-1*/rno-miR- miR-331-3p/rno-miR-331
24-1* hsa-miR-574-5p/mmu- High hsa-miR-29c/mmu- Low miR-574-5p
miR-29c/rno-miR-29c hsa-miR-424 High hsa-miR-146a/mmu- Low
hsa-miR-152/mmu-miR- High miR-146a/rno-miR- 152/rno-miR-152 146a
hsa-miR-302a/mmu-miR- High hsa-miR-154/mmu- Low 302a
miR-154/rno-miR-154 hsa-miR-181a/mmu-miR- High hsa-miR-215 Low
181a/rno-miR-181a hsa-miR-487b/mmu- Low hsa-miR-509-5p High
miR-487b/rno-miR- hsa-let-7d/mmu-let- High 487b 7d/rno-let-7d
hsa-miR-155 Low hsa-miR-18b High hsa-miR-620 High
hsa-miR-18a/mmu-miR- High 18a/rno-miR-18a hsa-miR-298 High
hsa-miR-98/mmu-miR- High 98/rno-miR-98 hsa-miR-576-3p High
hsa-miR-374b/mmu-miR- High 374/rno-miR-374 hsa-miR-32* High
hsa-miR-302d* High ebv-miR-BART17-5p High hsa-miR-620 High
hsa-miR-665 High hsa-miR-185/mmu-miR- High 185/rno-miR-185
hsa-miR-34b/mmu-miR- High 34b-3p hsa-let-7e/mmu-let- High
7e/rno-let-7e
TABLE-US-00011 TABLE 11 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from all other B cell malignancies in
the study ABC vs ABC miRNA list others hsa-miR-126/mmu-miR- High
126-3p/rno-miR-126 hsa-miR-125b/mmu-miR- High
125b-5p/rno-miR-125b-5p hsa-miR-145/mmu-miR- High 145/rno-miR-145
hsa-miR-22/mmu-miR- High 22/rno-miR-22 hsa-miR-21/mmu-miR- High
21/rno-miR-21 hsa-miR-199a-3p/hsa-miR- High 199b-3p/mmu-miR-199a-
3p/mmu-miR-199b/rno- miR-199a-3p hsa-miR-24/mmu-miR- High
24/rno-miR-24 hsa-miR-143/mmu-miR- High 143/rno-miR-143
hsa-miR-23b/mmu-miR- High 23b/rno-miR-23b hsa-miR-23a/mmu-miR- High
23a/rno-miR-23a hsa-miR-142-3p/mmu-miR- High 142-3p/rno-miR-142-3p
hsa-let-7a/mmu-let-7a/rno- High let-7a hsa-miR-146b-5p/mmu- High
miR-146b/rno-miR-146b hsa-miR-27a/mmu-miR- High 27a/rno-miR-27a
hsa-miR-30a/mmu-miR- High 30a/rno-miR-30a hsa-miR-27b/mmu-miR- High
27b/rno-miR-27b hsa-let-7c/mmu-let-7c/rno- High let-7c hsa-miR-921
Low ebv-miR-BHRF1-2 Low hsa-miR-199a-5p/mmu- Low
miR-199a-5p/rno-miR- 199a-5p hsa-miR-768-5p Low hsa-miR-491-3p Low
hsa-miR-185/mmu-miR- Low 185/rno-miR-185 ebv-miR-BART17-5p Low
hsa-miR-32* Low hsa-miR-335/mmu-miR- Low 335-5p/rno-miR-335
hsa-miR-149* Low hsa-miR-576-3p Low hsa-miR-214/mmu-miR- Low
214/rno-miR-214 hsa-miR-184/mmu-miR- Low 184/rno-miR-184
hsa-miR-520d-5p Low hsa-miR-518c* Low hsa-miR-801/mmu-miR-801 Low
hsa-miR-298 Low hsa-miR-634 Low hsa-miR-583 Low hsa-miR-187* Low
hsa-miR-30d/mmu-miR- Low 30d/rno-miR-30d hsa-miR-129-5p/mmu-miR-
Low 129-5p/rno-miR-129 hsa-miR-300 Low hsa-miR-620 Low hsa-miR- Low
130b*/mmu-miR- 130b* hsa-miR-20b/mmu- Low miR-20b/rno-miR- 20b-5p
kshv-miR-K12-3 Low hsa-miR-28-3p/rno- Low miR-28* hsa-miR-557 Low
hsa-miR-150/mmu- Low miR-150/rno-miR- 150 hsa-miR-98/mmu- Low
miR-98/rno-miR-98 hsa-miR-486- Low hsa-miR-518a- 5p/hsa-miR-527
hsa-miR-302d* Low hsa-miR-516a-5p Low hsa-miR-148b/mmu- Low
miR-148b/rno-miR- 148b-3p hsa-miR-25* Low hsa-miR-374b/mmu- Low
miR-374/rno-miR- 374 hsa-miR-638 Low hsa-miR-302a/mmu- Low miR-302a
hsa-miR-99b*/mmu- Low miR-99b*/rno-miR- 99b* hsa-miR-29c*/mmu- Low
miR-29c*/rno-miR- 29c* hsa-miR-138/mmu- Low miR-138/rno-miR- 138
hsa-miR-766 Low hsa-miR-488 Low hsa-miR-498 Low hsa-miR-339- Low
5p/mmu-miR-339- 5p/rno-miR-339-5p hsa-miR-193b* Low hsa-miR-299-
Low 5p/mmu-miR- 299*/rno-miR-299 ebv-miR-BART8* Low
hsa-miR-107/mmu- Low miR-107/rno-miR- 107 hsa-miR-519e* Low
hsa-miR-146b-3p Low hsa-miR-552 Low hsa-miR-509-5p Low hsa-miR-574-
Low 5p/mmu-miR-574- 5p hsa-miR-524-5p Low mghv-miR-M1-7-5p Low
hsa-miR-659 Low hcmv-miR-UL148D Low hsa-miR-92a/mmu- Low
miR-92a/rno-miR- 92a hsa-miR- Low 30e*/mmu-miR- 30e*/rno-miR- 30e*
hsa-miR- Low 183*/mmu-miR- 183* hsa-miR-144* Low hsa-miR-574- Low
3p/mmu-miR-574- 3p hsa-miR-889 Low hsa-miR-525-5p Low
kshv-miR-K12-8 Low hsa-miR-32/mmu- Low miR-32/rno-miR- 32
hsa-miR-938 Low hsa-miR-198 Low hsa-miR- Low 186/mmu-miR-
186/rno-miR-186 hsa-miR- Low 18a/mmu-miR- 18a/rno-miR-18a
hsa-miR-516b Low hsa-miR-625* Low hsa-miR-551b* Low hsa-miR-885-5p
Low hsa-miR-891a Low hsa-miR- Low 340/mmu-miR- 340-5p/rno-miR-
340-5p hsa-let-7d/mmu- Low let-7d/rno-let-7d hsa-miR-151- Low
5p/mmu-miR-151- 5p/rno-miR-151 hsa-miR-18b Low ebv-miR-BHRF1-1 Low
hsa-miR-510 Low hsa-miR-625 Low mghv-miR-M1-8 Low ebv-miR-BART19-
Low 3p hsa-miR-147 Low hsa-miR-28- Low 5p/mmu-miR- 28/rno-miR-28
ebv-miR-BART13 Low hsa-miR-25/mmu- Low miR-25/rno-miR- 25
hsa-miR-519d Low hsa-miR-361- Low 5p/mmu-miR- 361/rno-miR-361
hsa-miR-331- Low 3p/mmu-miR-331- 3p/rno-miR-331 hsa-miR-423- Low
3p/mmu-miR-423- 3p/rno-miR-423 hsa-miR-93/mmu- Low miR-93/rno-miR-
93
TABLE-US-00012 TABLE 12 Predictor microRNAs that distinguish
chronic lymphocytic leukemia from all other B cell malignancies in
the study CLL vs CLL miRNA list others hsa-miR- High 30e*/mmu-miR-
30e*/rno-miR-30e* hsa-miR-32/mmu- High miR-32/rno-miR-32
hsa-let-7g/mmu- High let-7g hsa-miR-186/mmu- High miR-186/rno-miR-
186 hsa-miR-140- High 5p/mmu-miR- 140/rno-miR-140 hsa-miR- High
196a*/mmu-miR- 196a*/rno-miR- 196a* hsa-miR- High 487b/mmu-miR-
487b/rno-miR-487b hsa-miR-150/mmu- High miR-150/rno-miR- 150
hsa-miR-147 High hsa-miR-486- High 5p/mmu-miR-486 hsa-miR-144* High
hsa-miR-154/mmu- High miR-154/rno-miR- 154 hsa-miR-28- High
5p/mmu-miR- 28/rno-miR-28 hsa-miR-299- High 5p/mmu-miR-
299*/rno-miR-299 hsa-miR-33a/mmu- High miR-33/rno-miR-33
hsa-miR-363/mmu- High miR-363/rno-miR- 363 hsa-miR-891a High
hsa-miR-768-5p High hsa-miR-361- High 5p/mmu-miR- 361/rno-miR-361
hsa-miR-519d High hsa-miR-335/mmu- High miR-335-5p/rno- miR-335
hsa-miR-668/mmu- High miR-668 hsa-let-7f/mmu-let- High
7f/rno-let-7f hsa-miR-24- High 1*/mmu-miR-24- 1*/rno-miR-24-1*
hsa-miR-223/mmu- High miR-223/rno-miR- 223 hsa-miR-140- High
3p/mmu-miR- 140*/rno-miR-140* hsa-miR-144/mmu- High
miR-144/rno-miR- 144 hsa-miR-638 High hsa-miR-30d/mmu- High
miR-30d/rno-miR- 30d hsa-miR-423- High 3p/mmu-miR-423-
3p/rno-miR-423 hsa-miR-155 High hsa-miR-101/mmu- High
miR-101a/rno-miR- 101a hsa-miR-20b/mmu- High miR-20b/rno-miR-
20b-5p hsa-miR-374a High hsa-miR-25/mmu- High miR-25/rno-miR-25
hsa-miR-199a- High 5p/mmu-miR-199a- 5p/rno-miR-199a- 5p hsa-miR-649
High hsa-miR-191/mmu- High miR-191/rno-miR- 191 hsa-miR-30e/mmu-
High miR-30e/rno-miR- 30e hsa-miR-107/mmu- High miR-107/rno-miR-
107 hsa-miR-93/mmu- High miR-93/rno-miR-93 hsa-miR-29c/mmu- High
miR-29c/rno-miR- 29c hsa-miR-541* High hsa-miR-888* High
hsa-miR-549 High hsa-miR-19a/mmu- High miR-19a/rno-miR- 19a
hsa-miR-342- High 3p/mmu-miR-342- 3p/rno-miR-342-3p hsa-miR-142-
High 5p/mmu-miR-142- 5p/rno-miR-142-5p hsa-miR-801/mmu- High
miR-801 hsa-let-7i/mmu-let- High 7i/rno-let-7i hsa-miR-26a/mmu-
High miR-26a/rno-miR- 26a hsa-miR-15a/mmu- High miR-15a
hsa-miR-195/mmu- High miR-195/rno-miR- 195 hsa-miR- High
106b/mmu-miR- 106b/rno-miR-106b hsa-miR-26b/mmu- High
miR-26b/rno-miR- 26b hsa-miR-15b/mmu- High miR-15b/rno-miR- 15b
hsa-miR-222/mmu- High miR-222/rno-miR- 222 hsa-miR-185/mmu- High
miR-185/rno-miR- 185 hsa-miR-550 High hsa-let-7e/mmu- Low
let-7e/rno-let-7e hsa-miR-24/mmu- Low miR-24/rno-miR-24
hsa-miR-30c- Low 2*/mmu-miR-30c- 2*/rno-miR-30c-2* hsa-miR-765 Low
mghv-miR-M1-4 Low hsa-miR-933 Low hsa-miR-620 Low hsa-miR-30b* Low
hsa-miR-658 Low hsa-miR-10a/mmu- Low miR-10a/rno-miR- 10a-5p
hsa-miR-665 Low hsa-miR-185* Low hsa-miR-503 Low
hsa-miR-126*/mmu-miR- Low 126-5p/rno-miR-126* hsa-miR-10b/mmu-miR-
Low 10b/rno-miR-10b hsa-miR-628-3p Low hsa-miR-422a Low
hsa-miR-193a-5p Low hsa-miR-143/mmu-miR- Low 143/rno-miR-143
hsa-miR-371-5p Low hsa-miR-100/mmu-miR- Low 100/rno-miR-100
hsa-miR-365/mmu-miR- Low 365/rno-miR-365 hsa-miR-145/mmu-miR- Low
145/rno-miR-145 kshv-miR-K12-6-3p Low ebv-miR-BART6-3p Low
hsa-miR-220c Low hsa-miR-519c-5p/hsa-miR- Low
519b-5p/hsa-miR-523*/hsa- miR-518e*/hsa-miR- 522*/hsa-miR-519a*
hsa-miR-130a/mmu-miR- Low 130a/rno-miR-130a hsa-miR-424 Low
hsa-miR-483-5p Low hsa-miR-193b Low hsa-miR-637 Low hsa-miR-920 Low
hsa-miR-488 Low ebv-miR-BHRF1-2 Low hsa-miR-526b Low
hsa-miR-126/mmu-miR-126- Low 3p/rno-miR-126 hsa-miR-943 Low
hsa-miR-199a-3p/hsa-miR- Low 199b-3p/mmu-miR-199a-
3p/mmu-miR-199b/rno-miR- 199a-3p mghv-miR-M1-3 Low hsa-miR-934 Low
hsa-miR-886-5p Low hsa-miR-200b*/mmu-miR- Low 200b*
hsa-miR-485-3p/mmu-miR- Low 485* hsa-miR-181a/mmu-miR- Low
181a/rno-miR-181a hsa-miR-125b/mmu-miR- Low
125b-5p/rno-miR-125b-5p
TABLE-US-00013 TABLE 13 Predictor microRNAs that distinguish
Hodgkin's lympoma from all other B cell malignancies in the study
HL vs HL miRNA list others hsa-miR-338- High 5p/mmu-miR-338-
5p/rno-miR-338* hsa-miR-433/mmu- High miR-433/rno-miR- 433
hsa-miR-552 High hsa-miR-202 High hsa-miR-299-3p High
hsa-miR-509-3-5p High hsa-miR-490-5p High hsa-miR-508-5p High
hsa-miR-181a-2* High hsa-miR-663 High hsa-miR-326/mmu- High
miR-326/rno-miR- 326 hsa-miR-542- High 3p/mmu-miR-542-
3p/rno-miR-542-3p hsa-miR-492 High hsa-miR-584 High hsa-miR-654-5p
High ebv-miR-BART20-3p High hsa-miR-542- High 5p/mmu-miR-542-
5p/rno-miR-542-5p ebv-miR-BART9* High hsa-miR-124/mmu- High
miR-124/rno-miR- 124 hsa-miR-551a High hsa-miR-208a/mmu- High
miR-208a/rno-miR- 208 hsa-miR-220b High hsa-miR-615- High
3p/mmu-miR-615-3p hsa-miR- High 135a*/mmu-miR- 135a* hiv1-miR-H1
High hsa-miR-124*/mmu- High miR-124*/rno-miR- 124* hsa-miR-502-5p
High hsa-miR-92b* High hsa-miR-518a-3p High hsa-miR-377* High
hsa-miR-125a- High 3p/mmu-miR-125a- 3p/rno-miR-125a-3p hsa-miR-30c-
High 1*/mmu-miR-30c- 1*/rno-miR-30c-1* hsa-miR-650 High hsa-miR-629
High hsa-miR-296- High 3p/mmu-miR-296- 3p/rno-miR-296
hsa-miR-425*/mmu- High miR-425* hsa-miR-514 High hsa-miR-519e High
hsa-miR-938 High hsa-miR-340*/mmu- High miR-340-3p/rno- miR-340-3p
hsa-miR-657 High hsa-miR-9*/mmu- High miR-9*/rno-miR-9*
ebv-miR-BART7* High hsa-miR-612 High hsa-miR-640 High hsa-miR-623
High hsa-miR-99b*/mmu- High miR-99b*/rno-miR- 99b* hsa-miR-645 High
hsa-miR-484/mmu- High miR-484/rno-miR- 484 hsa-miR-376a* High
hsa-miR-345 High hsa-miR-586 High hsa-miR-622 High hsa-miR-206/mmu-
High miR-206/rno-miR- 206 hcmv-miR-US25-1* High hsa-miR-302c* High
hsa-miR- High 106b*/mmu-miR- 106b*/rno-miR- 106b* hsa-miR-500 High
hsa-miR-890 High hsa-miR-10a*/mmu- High miR-10a*/rno-miR- 10a-3p
kshv-miR-K12-1 High hsa-miR-629* High hsa-miR-193b* High
ebv-miR-BHRF1-3 High hsa-miR-183/mmu- High miR-183/rno-miR- 183
hsa-let-7b*/mmu- High let-7b*/rno-let-7b* hsa-miR-409- High
5p/mmu-miR-409- 5p/rno-miR-409-5p hsa-miR-585 High hsa-miR-526b*
High hsa-miR-337-3p High hsa-miR-212/mmu- High miR-212/rno-miR- 212
hsa-miR-548b-3p High hcmv-miR-UL112 High hsa-miR-601 High
hsa-let-7d*/mmu- High let-7d*/rno-let-7d* hsa-miR-181b/mmu- High
miR-181b/rno-miR- 181b hsa-miR-195* High kshv-miR-K12-5 High
hsa-miR-500* High hsa-miR-24- High 2*/mmu-miR-24- 2*/rno-miR-24-2*
hsa-miR-382/mmu- High miR-382/rno-miR- 382 ebv-miR-BART8* High
hsa-miR-125b- High 2*/rno-miR-125b* hsa-miR-194* High
hsa-miR-297/mmu- High miR-297a hsa-miR-610 High hsa-miR-575 High
hsa-miR-21* High hsa-miR-936 High kshv-miR-K12-6-5p High
hsa-miR-553 High hsa-miR-652/mmu- High miR-652/rno-miR- 652
hsa-miR-877/mmu- High miR-877/rno-miR- 877 hsa-miR-526a/hsa- High
miR-520c-5p/hsa- miR-518d-5p hsa-miR-122* High hsa-miR-576-5p High
mghv-miR-M1-6 High hsa-miR-551b* High hsa-miR-125b- High
1*/mmu-miR-125b- 3p/rno-miR-125b- 3p hsa-miR-137/mmu- High
miR-137/rno-miR- 137 ebv-miR-BART18-3p High hsa-miR-452 High
hsa-miR-23a*/rno- High miR-23a* hsa-miR-617 High hsa-miR-550* High
hsa-miR-557 High hsa-miR-331- High 5p/mmu-miR-331- 5p hsa-miR-296-
High 5p/mmu-miR-296- 5p/rno-miR-296* mghv-miR-M1-2 High
ebv-miR-BART6-3p High hsa-miR-518b High hsa-miR-99b/mmu- High
miR-99b/rno-miR- 99b hsa-miR-525-5p High hsa-miR-589 High
hsa-miR-7-2* High hsa-miR-490- High 3p/mmu-miR-490 hsa-miR- High
150*/mmu-miR- 150* hsa-miR-17*/rno- High miR-17-3p hsa-miR-509-3p
High ebv-miR-BHRF1-1 High hsa-miR- High 183*/mmu-miR- 183*
hsa-miR-635 High hsa-miR- High 130b/mmu-miR- 130b/rno-miR-130b
mghv-miR-M1-8 High hsa-miR-887 High hsa-miR-210/mmu- High
miR-210/rno-miR- 210 hsa-miR-766 High hsa-miR-671- High
5p/mmu-miR-671- 5p hsa-miR-659 High hsa-miR-330- High 5p/mmu-miR-
330/rno-miR-330 hsa-miR-323- High 3p/mmu-miR-323- 3p/rno-miR-323
ebv-miR-BART13 High ebv-miR-BART5 High hsa-miR-602 High
hcmv-miR-UL148D High hsa-miR-373* High hsa-miR-526b High
hsa-miR-328/mmu- High miR-328/rno-miR- 328 hsa-miR-874/mmu- High
miR-874/rno-miR- 874 ebv-miR-BART19-3p High hsa-miR-595 High
hsa-miR-889 High mghv-miR-M1-7-5p High hsa-miR-483-5p High hsa-miR-
High 487b/mmu-miR- 487b/rno-miR-487b hsa-miR-675 High hsa-miR-220c
High
mghv-miR-M1-7-3p High hsa-miR-485- High 3p/mmu-miR-485* hsa-miR-198
High hsa-miR-483-3p High hcmv-miR-UL70-3p High hsa-miR-149/mmu-
High miR-149 hsa-miR-516a-5p High hsa-miR- High 145*/mmu-miR- 145*
hsa-miR-656 High hsa-miR-502-3p High hsa-miR-29c*/mmu-miR- High
29c*/rno-miR-29c* hsa-miR-937 High hsa-miR-515-5p High
hsa-miR-153/mmu-miR- High 153/rno-miR-153 hsa-miR-519e* High
hsa-miR-128/mmu-miR- High 128/rno-miR-128 hsa-miR-516b High
hsa-miR-532-5p/mmu-miR- High 532-5p/rno-miR-532-5p kshv-miR-K12-8
High hsa-miR-455-3p High hsa-miR-27a*/mmu-miR- High
27a*/rno-miR-27a* hsa-miR-510 High hsa-miR-505/rno-miR-505 High
hsa-miR-187* High hsa-miR-498 High hsa-miR-625 High
hsa-miR-129-5p/mmu-miR- High 129-5p/rno-miR-129 ebv-miR-BHRF1-2
High hsa-miR-143* High kshv-miR-K12-3 High hsa-miR-660 High
hsa-miR-25* High hsa-miR-29a*/mmu-miR- High 29a*/rno-miR-29a*
hsa-miR-422a High hsa-miR-518a-5p/hsa-miR- High 527
hsa-miR-519c-5p/hsa-miR- High 519b-5p/hsa-miR-
523*/hsa-miR-518e*/hsa- miR-522*/hsa-miR-519a*
hsa-miR-28-3p/rno-miR-28* High hsa-miR-300 High
hsa-miR-130a/mmu-miR- High 130a/rno-miR-130a hsa-miR-583 High
hsa-miR-149* High hsa-miR-184/mmu-miR- High 184/rno-miR-184
hsa-miR-625* High hsa-miR-99a/mmu-miR- High 99a/rno-miR-99a
hsa-miR-199b-5p High hsa-miR-513a-5p High hsa-miR-494/mmu-miR- High
494/rno-miR-494 mghv-miR-M1-4 High hsa-miR-634 High hsa-miR-923
High hsa-miR-503 High ebv-miR-BART2-3p High hsa-miR-520d-5p High
hsa-miR-30b* High hsa-miR-30c-2*/mmu-miR- High
30c-2*/rno-miR-30c-2* hsa-miR-658 High hsa-miR-921 High
hsa-miR-423-5p/mmu-miR- High 423-5p hsa-miR-933 High
hsa-miR-23b/mmu-miR- Low 23b/rno-miR-23b hsa-miR-27b/mmu-miR- Low
27b/rno-miR-27b hsa-miR-550 Low hsa-let-7a/mmu-let-7a/rno- Low
let-7a hsa-miR-24/mmu-miR- Low 24/rno-miR-24 hsa-miR-451/mmu-miR-
Low 451/rno-miR-451 hsa-miR-30a/mmu-miR- Low 30a/rno-miR-30a
hsa-miR-20b/mmu-miR- Low 20b/rno-miR-20b-5p hsa-miR-26a/mmu-miR-
Low 26a/rno-miR-26a hsa-miR-26b/mmu-miR- Low 26b/rno-miR-26b
hsa-miR-101/mmu-miR- Low 101a/rno-miR-101a hsa-miR-106b/mmu-miR-
Low 106b/rno-miR-106b hsa-miR-16/mmu-miR- Low 16/rno-miR-16
hsa-miR-29b/mmu-miR- Low 29b/rno-miR-29b hsa-miR-768-3p Low
hsa-miR-30e/mmu-miR- Low 30e/rno-miR-30e hsa-miR-106a Low
hsa-miR-142-5p/mmu-miR- Low 142-5p/rno-miR-142-5p
hsa-miR-144/mmu-miR- Low 144/rno-miR-144 hsa-miR-17/mmu-miR- Low
17/rno-miR-17-5p/rno-miR- 17 hsa-miR-15b/mmu-miR- Low
15b/rno-miR-15b hsa-miR-30c/mmu-miR- Low 30c/rno-miR-30c
hsa-miR-142-3p/mmu-miR- Low 142-3p/rno-miR-142-3p
hsa-miR-20a/mmu-miR- Low 20a/rno-miR-20a hsa-miR-30b/mmu-miR- Low
30b/rno-miR-30b-5p hsa-miR-19a/mmu-miR- Low 19a/rno-miR-19a
hsa-miR-19b/mmu-miR- Low 19b/rno-miR-19b
[0172] From the data in Tables 10 through 13, we identified subsets
of microRNAs that are sufficient to distinguish each of the six B
cell malignancies from the other five. We selected only microRNAs
that are expressed more highly in the selected B cell malignancy.
Those microRNAs are listed in Tables 14 and 15. In certain
embodiments, each of the microRNAs listed in Tables 14 and 15 can
be used to distinguish one B cell malignancy from the other
five.
TABLE-US-00014 TABLE 14 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL, germinal center B-cell like (GCB)
DLBCL, and Burkitt lymphoma ABC High GCB High BL High
hsa-miR-22/mmu-miR-22/rno-miR- hsa-miR-93/mmu-miR-93/rno-miR-93
hsa-miR-628- 22 hsa-miR-103/mmu-miR-103/rno-miR-103 3p
hsa-miR-21/mmu-miR-21/rno-miR- hsa-miR-320/mmu-miR-320/rno-miR-320
21 hsa-miR-125a-5p/mmu-miR-125a-5p/rno-miR-
hsa-miR-24/mmu-miR-24/rno-miR- 125a-5p 24
hsa-miR-23b/mmu-miR-23b/rno- miR-23b hsa-miR-23a/mmu-miR-23a/rno-
miR-23a hsa-let-7a/mmu-let-7a/rno-let-7a
hsa-let-7c/mmu-let-7c/rno-let-7c
TABLE-US-00015 TABLE 15 Predictor microRNAs that distinguish
chromic lymphocytic leukemia, follicular lymphoma, and Hodgkin's
lymphoma CLL High FL High HL High hsa-miR-32/mmu-miR-32/rno-
hsa-miR-152/mmu-miR- hsa-miR-498 miR-32 152/rno-miR-152
hsa-miR-525-5p hsa-miR-150/mmu-miR-150/rno- hsa-miR-885-5p
hsa-miR-551b* miR-150 hsv1-miR-H1 hsa-miR-340*/mmu-miR-340-
hsa-miR-140-5p/mmu-miR- hsa-miR-548d-5p 3p/rno-miR-340-3p
140/rno-miR-140 hsa-miR-488 hsa-miR-494/mmu-miR-494/rno-
hsa-let-7g/mmu-let-7g ebv-miR-BART16 miR-494
hsa-miR-154/mmu-miR-154/rno- hsa-miR-22*/mmu-miR-
hsa-miR-183*/mmu-miR-183* miR-154 22*/rno-miR-22* hsa-miR-659
hsa-miR-486-5p/mmu-miR-486 hsa-miR-513a-3p hsa-miR-193b*
hsa-miR-101/mmu-miR- hsa-miR-708/mmu-miR- hsa-miR-766
101a/rno-miR-101a 708/rno-miR-708 hsa-miR-516a-5p
hsa-miR-30e/mmu-miR-30e/rno- hsa-miR-425/mmu-miR-
hsa-miR-125b-1*/mmu-miR- miR-30e 425/rno-miR-425
125b-3p/rno-miR-125b-3p hsa-miR-768-5p hsa-miR-337-3p
ebv-miR-BART6-3p hsa-miR-363/mmu-miR-363/rno- ebv-miR-BART17-5p
ebv-miR-BART8* miR-363 hsa-miR-221* hsa-miR-509-3-5p
hsa-miR-668/mmu-miR-668 hsa-miR-92b/mmu-miR- hsa-miR-602
hsa-miR-147 92b/rno-miR-92b ebv-miR-BHRF1-1 hsa-miR-196a*/mmu-miR-
hsa-miR-197/mmu-miR-197 mghv-miR-M1-2 196a*/rno-miR-196a*
hsa-miR-32* hsa-miR-145*/mmu-miR-145* hsa-miR-142-5p/mmu-miR-142-
hsa-miR-342-5p/mmu-miR- hsa-miR-296-5p/mmu-miR-296-
5p/rno-miR-142-5p 342-5p/rno-miR-342-5p 5p/rno-miR-296*
hsa-miR-199a-5p/mmu-miR- hsa-miR-524-5p hsa-miR-17*/rno-miR-17-3p
199a-5p/rno-miR-199a-5p hsa-miR-34c-5p/mmu-miR- hsa-miR-452
hsa-miR-24-1*/mmu-miR-24- 34c/rno-miR-34c
hsa-miR-326/mmu-miR-326/rno- 1*/rno-miR-24-1*
hsa-let-7e/mmu-let-7e/rno-let- miR-326 hsa-miR-891a 7e
hsa-miR-652/mmu-miR-652/rno- hsa-miR-550 hsa-miR-151-3p miR-652
hsa-miR-801/mmu-miR-801 hsa-miR-744/mmu-miR-744 hsa-miR-623
hsa-miR-549 hsa-miR-574-3p/mmu-miR- hsa-miR-194* hsa-miR-888*
574-3p hsa-miR-125a-3p/mmu-miR- hsa-miR-600 125a-3p/rno-miR-125a-3p
hsa-miR-20b* hsa-miR-10a*/mmu-miR- hsa-miR-194/mmu-miR-
10a*/rno-miR-10a-3p 194/rno-miR-194 hsa-miR-519e
hsa-miR-363*/rno-miR-363* hsa-miR-502-5p hsa-miR-155*
hsa-miR-124*/mmu-miR- hsa-miR-34b/mmu-miR-34b-3p 124*/rno-miR-124*
hsa-miR-922 hsa-miR-345 hsa-miR-497/mmu-miR- hsa-miR-584
497/rno-miR-497 hsa-miR-650 hsa-miR-493 hsa-miR-202
hsa-miR-138/mmu-miR- hsa-miR-548b-3p 138/rno-miR-138 hsa-miR-492
hsa-miR-215 hsa-miR-135a*/mmu-miR-135a* hsa-miR-302a/mmu-miR-302a
ebv-miR-BART20-3p hsa-miR-96/mmu-miR-96/rno- hsa-miR-586 miR-96
hsa-miR-338-5p/mmu-miR-338- hsa-miR-218-2*/mmu-miR- 5p/rno-miR-338*
218-2*/rno-miR-218* hsa-miR-92b* kshv-miR-K12-7 hiv1-miR-H1
hsa-miR-301a/mmu-miR- hsa-miR-508-5p 301a/rno-miR-301a
hsa-miR-542-5p/mmu-miR-542- 5p/rno-miR-542-5p hsa-miR-490-5p
hsa-miR-663 hsa-miR-433/mmu-miR-433/rno- miR-433
[0173] Finally, we identified sets of microRNAs that can
distinguish between each pair of B cell malignancies in the study.
Lists of those microRNAs are shown in Appendix B, Tables 16 to 30.
In certain embodiments, each of the listed microRNAs is sufficient
to distinguish between the two B cell malignancies listed in the
table description.
[0174] The consistent expression of a number of miRNAs in a diverse
set of B cell malignances suggests a role for miRNAs in the
maintenance of tumor phenotype. Assays for stage-specific B cell
markers such as BCL6, a marker for GC cells, are essential in the
clinical diagnosis of B cell malignancies. Our data suggest that
stage-specific biology in B cell malignancies is retained at the
miRNA level. Recent work has demonstrated the utility of gene
expression profiling in reliably distinguishing closely related B
cell malignancies. See Hummel et al. N Engl J Med. 2006;
354:2419-2430; Dave et al. N Engl J Med. 2006; 354:2431-2442.
However, clinical translation of gene expression profiling has
proved to be difficult because of the need for freshly frozen
tissue. Since intact miRNAs can be isolated from tissues preserved
using standard methods (see, e.g., Doleshal et al. J Mol Diagn.
2008; 10:203-211; Xi et al. Rna. 2007; 13:1668-1674), diagnostic
methods based upon miRNA profiles could be fairly easy to translate
to clinical use.
[0175] Interestingly, in contrast to a previous study (Lu et al.
Nature 2005; 435:834-838), we did not note a decrease in the
expression of the total number or overall expression levels of
miRNAs in B cell malignancies compared to normal lymph nodes.
Although B cell malignancies maintain the expression of a number of
stage-specific miRNAs, their miRNA expression patterns are clearly
deranged compared to normal lymph nodes. The consequences of
altered miRNA expression in B cell tumors would be important to
explore in future studies.
[0176] In conclusion, our study demonstrates that mature B cell
subsets have distinct patterns of microRNA expression, suggesting a
role for miRNAs in B cell differentiation. We provide experimental
evidence that transcription factors such as LM02 and PRDM1 are
direct targets of differentially expressed miRNAs. B cell
malignancies demonstrate a distinct pattern of miRNA expression
that could be useful in distinguishing morphologically identical
subtypes of these tumors. The conserved expression of
stage-specific microRNAs in normal and malignant B cells suggests a
role for microRNAs in the maintenance of the mature B cell
phenotype.
Example 6
Differential Expression of miRNAs in ABC DLBCL Vs. GBC DLBCL
Malignancies
[0177] As discussed above, microRNAs have shown promise as
biomarkers in a number of malignancies. Diffuse large B cell
lymphoma (DLBCL) is the most common form of lymphoma and is known
to comprise at least 2 molecularly distinct subgroups with
different responses to standard therapy. These two distinct
subgroups are typically identified as ABC DLBCL and GC (or GCB)
DLBCL.
[0178] However, the current methods used to distinguish GCB from
non GCB DLBCL are limited fashion and can yield inconsistent
results. While gene expression profiling remains the gold standard
for distinguishing these 2 molecular subgroups it is not routinely
performed in clinical laboratories. In an effort to expand and
improve the existing analytical options we sought to develop
microRNA-based assays. We created RNA libraries from 31 different
samples and performed deep sequencing analysis to identify the
present miRNAs.
[0179] Small RNA Library Creation and Deep Sequencing
[0180] Total RNA was extracted from the 31 samples using the
phenol-chloroform method to preserve microRNAs, using Ambion
reagents. Total RNA (typically 5 .mu.g) from each sample was run on
denaturing polyacrylamide-urea gels. The .about.17-25 nucleotide
RNAs were excised from the gel, ligated to sequencing adaptors on
both ends and reverse transcribed. The resulting cDNA library was
PCR-amplified for 15 cycles and gel purified on 6% acrylamide gel.
The gel-purified amplicon quality and quantity were analyzed on a
6% acrylamide gel relative to oligonucleotides of known
concentration and size. 120 .mu.l of 1-4 .mu.M library were loaded
on to the Illumina cluster station, where DNA molecules were
attached to high-density universal adaptors in the flow cells and
amplified. The DNA clusters generated via this process were
sequenced with sequencing-by-synthesis technology, where successive
high-resolution images of the four-color fluorescence excitation
dependent on the base incorporated during each cycle were captured.
Sequencing reads were generated for each of the 31 samples and base
calls were rendered using Illumina software. All the primary
sequencing data and gene expression data is publicly available
through the GEO archive through accession GSE22898.
[0181] The small RNA libraries from the 31 samples which were
subjected to massively parallel, high-throughput sequencing using
the Illumina platform generated a total of 328 million separate
reads. Our approach to analyzing the sequences and discovering
microRNAs broadly follows a previously described method (see,
Zheng, Q., et al. GOEAST: a web-based software toolkit for Gene
Ontology enrichment analysis, Nucleic acids Res. 2008; 36 (Web
Server issue: W358-363). All bioinformatics analyses were performed
using a cluster of 1024 Linux computer nodes. Preprocessing was
carried out using locally written Shell and Perl scripts.
[0182] From the raw sequences generated by high-throughput
sequencing, the 3'- and 5'-adaptor sequences were trimmed. Low
quality sequences were identified as those sequencing reads that
contained stretches of consecutive identical bases or uncalled
nucleotides (N) in the first 12 bases and sequencing reads shorter
than 17 nucleotides. To minimize redundancy, reads were initially
curtailed to the first 22 nucleotides and identical sequences were
represented with a single fasta entry for analysis. Each unique
sequence was mapped to the reference genome (Ensembl, build 50) and
reads were filtered such that only perfect alignments (full length,
100% identity) were retained. Reads that aligned to more than five
positions in the genome and reads that overlapped with the UCSC RNA
genes were identified and excluded from microRNA analysis.
[0183] Identification and Analysis of the Captured miRNA
Sequences
[0184] Sequences that occurred 20 or more times in at least one
sample were consolidated and annotated for the 31 samples. Genomic
loci that overlapped with microRNAs described in miRBase (version
13) were identified as known microRNAs (see Table 31). The
remaining genomic loci were identified as encoding candidate novel
microRNAs (see Table 32).
[0185] The vast majority (96%) of the candidate novel microRNAs
were found in more than one sample, with only a small minority of
microRNAs were expressed exclusively in a specific B cell subset or
malignancy. Many of the microRNAs that we identified in normal and
malignant B cells were expressed at ten-fold or higher levels in
these non B cell cases. These findings suggest that the microRNAs
we have identified are broadly expressed and may have roles in a
number of diverse tissue types.
[0186] miRNA Profiling Using Real-Time PCR
[0187] miRNA expression profiling was conducted using the Applied
Biosystems 384-well multiplexed real-time PCR assay using 400 ng of
total RNA. Eight reactions, each containing 50 ng of RNA and a
multiplex looped primer pool with endogenous small nucleolar
(sno)-RNA controls, were used to reverse-transcribe the miRNAs in
parallel fashion (see Tables 33 and 34 for primers). The completed
reactions were loaded onto the 384-well plate per manufacturer's
instructions, and real-time PCR was run on the ABI 7900HT Prism
instrument. For each 384-well plate, we used the automatically
determined cycle-threshold (C.sub.T) using the SDS 2.2.1 software
(Applied Biosystems). Consistent with manufacturer recommendations,
a C.sub.T greater than 35 was treated as undetected. The probes
deemed to be present were normalized to the average expression of a
sno-RNA control. The expression values were calculated as
2.sup.-.DELTA.CT, then median centered to 500 and log
2-transformed.
[0188] For further validation of the deep sequencing results, we
selected candidate novel microRNAs (see Table 34) that were
detectably measured in at the sequencing data from least one of
four diffuse large B cell lymphoma (DLBCL) cases. Using stem-loop
reverse transcription (Ashburner, M., et al., Nat Genet. (2000);
25(1):25-29) for quantitative PCR, we tested the expression of the
candidate microRNAs in 101 primary tumors from patients with DLBCL
and found that about 92% were detectably measured by real-time PCR
in at least 10% of these cases, suggesting that real-time PCR
reproducibly identifies microRNAs that are expressed in lymphomas.
We also used real-time PCR to measure the expression of known
microRNAs (see Table 33) in the same 101 samples and found that
over 90% of these were also detected in at least 10% of the cases
using real-time PCR. We found that six of the seven RT-PCR
constructs that targeted RNA hairpins that had low probability of
being a microRNA resulted in no detectable signal. These results
suggest that our assays have high specificity for microRNAs and
that the computational predictions based on our sequencing data
correctly identified microRNAs.
[0189] Differentiating ABC DLBCL Vs. GCB DLBCL Malignancies
[0190] Gene expression profiling of patients with DLBCL has
demonstrated that the tumors comprise at least two distinct
diseases with different response rates to standard chemotherapy
regimens (Chen, C., et al., Nucleic Acids Res. (2005);
33(20):e179). We hypothesized that microRNAs might be used to make
this clinically important distinction for which gene expression
profiling remains the gold standard. We performed gene expression
profiling on 101 DLBCL cases and further subdivided these cases
into the molecular subgroups.
[0191] Tumor samples from 101 patients with diffuse large B cell
lymphoma were obtained at the time of diagnosis and freshly frozen.
These cases were profiled using Affymetrix Gene 1.0 ST arrays. The
molecular subgroups were distinguished using a Bayesian approach
described previously (Ambros, V., et al., RNA
(2003);9(3):277-279).
[0192] We found that 25 microRNAs with the highest t-statistic were
equally efficacious as the gene expression profiling in
differentiating the two groups of DLBCL with over 95% overlap
between the classifications rendered by the two methods, using
leave out one cross-validation (see Table 35). Interestingly, a
subset of these 25 predictor microRNAs was candidate novel
microRNAs, suggesting a biological and clinical relevance for these
candidate novel microRNAs in DLBCL tumors.
[0193] Our work provides an exhaustive identification of the
microRNAs in normal and malignant B cells; that is a prerequisite
to the delineation of their role. Further, we have developed a
comprehensive framework that spans the identification of microRNAs
from deep sequencing data to measuring their expression using
real-time PCR and validating their expression in primary human
tumors.
[0194] It is also conceivable that some of the low-abundance
microRNAs that we have identified in our study may be expressed at
higher levels in other development stages or in other cell types.
This notion is confirmed by our examination of the novel microRNAs
in non B cell data. For instance, a number of the microRNAs that we
discovered were also present at 10-fold or higher levels in cell
lines derived from breast cancer and cervical cancer, suggesting
that the microRNAs that we have discovered in B cells have broad
biological significance.
[0195] Deep Sequencing Reveals a Novel miRNA Cluster that Regulates
the TGF-.beta. Pathway
[0196] Although microRNAs appear to be distributed throughout the
genome, a number of microRNAs have been found in clusters such as
miR-17-92 that are transcribed from a single primary transcript and
cleaved into the individual microRNAs by the enzyme DROSHA. We
found 2 separate clusters of candidate novel microRNAs on
chromosome 9 and chromosome 14 (within the IgH locus),
respectively. The first cluster was previously annotated as a
hypothetical gene LOC100130622, and subsequently discarded from
Refseq when no associated protein was identified. Our data
demonstrate that this cluster (miR-2355), conserved only in
primates, encodes 6 separate microRNAs: has-miR-2355a-1,
has-miR-2356-1, has-miR-2355a-2, has-miR-2356-2, hsa-miR2355a-3,
and hsa-miR-2355b (see Table 32 for sequence).
[0197] In order to evaluate whether the microRNAs encoded in these
clusters originate from the same primary transcript, we took KMS12
multiple myeloma cells which express these microRNAs and used siRNA
to knock-down the expression of the microRNA processing enzyme
Drosha. This enzyme acts at the first step of microRNA processing
by cleaving microRNA precursors from the primary transcript. We
found that decreased Drosha expression was associated with
increased accumulation of primary transcripts of both the miR-17-92
cluster as well as the novel miR-2355 cluster. MicroRNAs from
miR-2355 cluster were found to be expressed more highly in normal
germinal center (GC) B cells compared to memory cells.
[0198] The microRNAs of this cluster all share the same seed
sequence, suggesting that they target the same genes. Among the
computationally predicted targets of this microRNA cluster, we
identified SMAD2 and SMAD3 which are well known mediators of the
TGF-beta signaling pathway. We noted that gene expression of both
SMAD2 and SMAD3 in our set of 101 DLBCLs were inversely correlated
with this cluster (P<0.001, correlation test). Gene set
enrichment analysis revealed that expression of the TGF-beta
pathway in DLBCL samples varied inversely with the expression of
the microRNA cluster, with a higher expression of the microRNA
associated with a lower expression of the pathway (P<10.sup.-6),
which has been noted as a factor in the biology of these
tumors.
TABLE-US-00016 TABLE 31 miRNAs identified by deep sequencing
analysis. Putative Mature/ miRBase miRBase SEQ Minor Mature/
Mature/Minor miRBase SEQ ID Mature Sequence miRNA miRBase Minor
Accession Mature/Minor ID NO. Captured ID ID ID Number Sequence NO.
1 CUGCGCAAGCUACUGCCUUGCU hsa-let- hsa-let- hsa-let- MIMAT0004585
CUGCGCAAGCUACUGCCUUGCU 393 7i* 7i 7i* 2 CCACGGAUGUUUGAGCAUGUGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004516 ACGGAUGUUUGAGCAUGUGCUA 394
105-1* 105-1 105* 3 CCACGGAUGUUUGAGCAUGUGC hsa-mir- hsa-mir-
hsa-miR- MIMAT0004516 ACGGAUGUUUGAGCAUGUGCUA 395 105-2* 105-2 105*
4 UACUGCAAUGUAAGCACUUCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004517
CUGCAAUGUAAGCACUUCUUAC 396 106a* 106a 106a* 5 CAUUAUUACUUUUGGUACGCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000444 CAUUAUUACUUUUGGUACGCG 397
126* 126 126* 6 AAGCCCUUACCCCAAAAAGUAU hsa-mir- hsa-mir- hsa-miR-
MIMAT0004548 AAGCCCUUACCCCAAAAAGUAU 398 129* 129-1 129* 7
AAGCCCUUACCCCAAAAAGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0004605
AAGCCCUUACCCCAAAAAGCAU 399 129-2* 129-2 129-3p 8
UCUACAGUGCACGUGUCUCCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000250
UCUACAGUGCACGUGUCUCCAG 400 139-5p 139 139-5p 9
CUGGUACAGGCCUGGGGGACAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004610
CUGGUACAGGCCUGGGGGACAG 401 150* 150 150* 10 ACUGCAGUGAAGGCACUUGUAG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000071 ACUGCAGUGAAGGCACUUGUAG 402
17* 17 17* 11 UGAAUUACCGAAGGGCCAUAA hsa-mir- hsa-mir- hsa-miR-
MIMAT0004560 GUGAAUUACCGAAGGGCCAUAA 403 183* 183 183* 12
AGGGGCUGGCUUUCCUCUGGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004611
AGGGGCUGGCUUUCCUCUGGUC 404 185* 185 185* 13 ACUGCCCUAAGUGCUCCUUCUGG
hsa-mir- hsa-mir- hsa-miR- MIMAT0002891 ACUGCCCUAAGUGCUCCUUCUGG 405
18a* 18a 18a* 14 AACUGGCCUACAAAGUCCCAGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000459 AACUGGCCUACAAAGUCCCAGU 406 193a-3p 193a 193a-3p 15
CGGGGUUUUGAGGGCGAGAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004767
CGGGGUUUUGAGGGCGAGAUGA 407 193b* 193b 193b* 16
CCCAGUGUUCAGACUACCUGUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000231
CCCAGUGUUCAGACUACCUGUUC 408 199a-2* 199a-2 199a-5p 17
UAGUUUUGCAUAGUUGCACUAC hsa-mir- hsa-mir- hsa-miR- MIMAT0004490
AGUUUUGCAUAGUUGCACUACA 409 19a* 19a 19a* 18 AGUUUUGCAGGUUUGCAUCCAGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004491 AGUUUUGCAGGUUUGCAUCCAGC 410
19b-1* 19b-1 19b-1* 19 ACUGUAGUAUGGGCACUUCCAG hsa-mir- hsa-mir-
hsa-miR- MIMAT0004752 ACUGUAGUAUGGGCACUUCCAG 411 20b* 20b 20b* 20
ACCUGGCAUACAAUGUAGAUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004568
ACCUGGCAUACAAUGUAGAUUU 412 221* 221 221* 21 GGGUUCCUGGCAUGCUGAUUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004587 UGGGUUCCUGGCAUGCUGAUUU 413
23b* 23b 23b* 22 AGGCGGAGACUUGGGCAAUUG hsa-mir- hsa-mir- hsa-miR-
MIMAT0004498 AGGCGGAGACUUGGGCAAUUG 414 25* 25 25* 23
AGAGCUUAGCUGAUUGGUGAAC hsa-mir- hsa-mir- hsa-miR- MIMAT0004588
AGAGCUUAGCUGAUUGGUGAAC 415 27b* 27b 27b* 24 CUGGGAGGUGGAUGUUUACUUC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004589 CUGGGAGGUGGAUGUUUACUUC 416
30b* 30b 30b* 25 CUGGGAGAGGGUUGUUUACUCC hsa-mir- hsa-mir- hsa-miR-
MIMAT0004674 CUGGGAGAGGGUUGUUUACUCC 417 30c-1* 30c-1 30c-1* 26
CUGGGAGAAGGCUGUUUACUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0004550
CUGGGAGAAGGCUGUUUACUCU 418 30c-2* 30c-2 30c-2* 27
CUUUCAGUCGGAUGUUUACAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000693
CUUUCAGUCGGAUGUUUACAGC 419 30e* 30e 30e* 28 UCCCUGUCCUCCAGGAGCUCACG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000764 UCCCUGUCCUCCAGGAGCUCACG 420
339-5p 339 339-5p 29 UCUCACACAGAAAUCGCACCCGU hsa-mir- hsa-mir-
hsa-miR- MIMAT0000753 UCUCACACAGAAAUCGCACCCGU 421 342-3p 342 342-3p
30 AAUCAGCAAGUAUACUGCCCUA hsa-mir- hsa-mir- hsa-miR- MIMAT0004557
CAAUCAGCAAGUAUACUGCCCU 422 34a* 34a 34a* 31 UCCCCCAGGUGUGAUUCUGAUUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004682 UCCCCCAGGUGUGAUUCUGAUUU 423
361-3p 361 361-3p 32 AAUCCUUGGAACCUAGGUGUGAGU hsa-mir- hsa-mir-
hsa-miR- MIMAT0000705 AAUCCUUGGAACCUAGGUGUGAGU 424 362-5p 362
362-5p 33 AGGGACUUUCAGGGGCAGCUGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0009199 AGGGACUUUCAGGGGCAGCUGU 425 365-2* 365-2 365* 34
CUUAUCAGAUUGUAUUGUAAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004688
CUUAUCAGAUUGUAUUGUAAUU 426 374a* 374a 374a* 35
CUUAGCAGGUUGUAUUAUCAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004956
CUUAGCAGGUUGUAUUAUCAUU 427 374b* 374b 374b* 36
AGGUUACCCGAGCAACUUUGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001638
AGGUUACCCGAGCAACUUUGCAU 428 409-5p 409 409-5p 37
CAAAACGUGAGGCGCUGCUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0004749
CAAAACGUGAGGCGCUGCUAU 429 424* 424 424* 38 UAUGUGCCUUUGGACUACAUCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0003150 UAUGUGCCUUUGGACUACAUCG 430
455-5p 455 455-5p 39 GUCAUACACGGCUCUCCUCUCU hsa-mir- hsa-mir-
hsa-miR- MIMAT0002176 GUCAUACACGGCUCUCCUCUCU 431 485-3p 485 485-3p
40 UGUCUUACUCCCUCAGGCACAU hsa-mir- hsa-mir- hsa-miR- MIMAT0003257
UGUCUUACUCCCUCAGGCACAU 432 550-1* 550-1 550* 41
UGUCUUACUCCCUCAGGCACAU hsa-mir- hsa-mir- hsa-miR- MIMAT0003257
UGUCUUACUCCCUCAGGCACAU 433 550-2* 550-2 550* 42
GAAAUCAAGCGUGGGUGAGACC hsa-mir- hsa-mir- hsa-miR- MIMAT0004794
GAAAUCAAGCGUGGGUGAGACC 434 551b* 551b 551b* 43
AUUCUAAUUUCUCCACGUCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0003241
AUUCUAAUUUCUCCACGUCUUU 435 576-5p 576 576-5p 44
UCAGAACAAAUGCCGGUUCCCAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0003256
UCAGAACAAAUGCCGGUUCCCAGA 436 589* 589 589* 45
GAGCUUAUUCAUAAAAGUGCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003258
GAGCUUAUUCAUAAAAGUGCAG 437 590-5p 590 590-5p 46
GGGGGUCCCCGGUGCUCGGAUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004804
GGGGGUCCCCGGUGCUCGGAUC 438 615-5p 615 615-5p 47
GACUAUAGAACUUUCCCCCUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0004808
GACUAUAGAACUUUCCCCCUCA 439 625* 625 625* 48 UGGUGGGCCGCAGAACAUGUGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0003330 UGGUGGGCCGCAGAACAUGUGC 440
654-5p 654 654-5p 49 AGGAAGCCCUGGAGGGGCUGGAG hsa-mir- hsa-mir-
hsa-miR- MIMAT0003880 AGGAAGCCCUGGAGGGGCUGGAG 441 671-5p 671 671-5p
50 AACUAGACUGUGAGCUUCUAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004927
CAACUAGACUGUGAGCUUCUAG 442 708* 708 708* 51 CAACAAAUCACAGUCUGCCAUA
hsa-mir- hsa-mir- hsa-miR- MIMAT0004553 CAACAAAUCACAGUCUGCCAUA 443
7-1* 7-1 7-1* 52 AUAAAGCUAGAUAACCGAAAGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000442 AUAAAGCUAGAUAACCGAAAGU 444 9-1* 9-1 9* 53
AUAAAGCUAGAUAACCGAAAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000442
AUAAAGCUAGAUAACCGAAAGU 445 9-2* 9-2 9* 54 AGGGACGGGACGCGGUGCAGUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0004792 AGGGACGGGACGCGGUGCAGUG 446
92b* 92b 92b* 55 AUAAAGCUAGAUAACCGAAAGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000442 AUAAAGCUAGAUAACCGAAAGU 447 9-3* 9-3 9* 56
UGAGGUAGUAGGUUGUAUAGUU hsa-let- hsa-let- hsa-let- MIMAT0000062
UGAGGUAGUAGGUUGUAUAGUU 448 7a-2 7a-2 7a 57 UGAGGUAGUAGGUUGUAUAGUU
hsa-let- hsa-let- hsa-let- MIMAT0000062 UGAGGUAGUAGGUUGUAUAGUU 449
7a-3 7a-3 7a 58 UGAGGUAGUAGGUUGUAUGGUU hsa-let- hsa-let- hsa-let-
MIMAT0000064 UGAGGUAGUAGGUUGUAUGGUU 450 7c 7c 7c 59
UGAGGUAGGAGGUUGUAUAGUU hsa-let- hsa-let- hsa-let- MIMAT0000066
UGAGGUAGGAGGUUGUAUAGUU 451 7e 7e 7e 60 UGAGGUAGUAGAUUGUAUAGUU
hsa-let- hsa-let- hsa-let- MIMAT0000067 UGAGGUAGUAGAUUGUAUAGUU 452
7f-1 7f-1 7f 61 UGAGGUAGUAGUUUGUACAGUU hsa-let- hsa-let- hsa-let-
MIMAT0000414
UGAGGUAGUAGUUUGUACAGUU 453 7g 7g 7g 62 UGAGGUAGUAGUUUGUGCUGUU
hsa-let- hsa-let- hsa-let- MIMAT0000415 UGAGGUAGUAGUUUGUGCUGUU 454
7i 7i 7i 63 AACCCGUAGAUCCGAACUUGUG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000098 AACCCGUAGAUCCGAACUUGUG 455 100 100 100 64
UACAGUACUGUGAUAACUGAA hsa-mir- hsa-mir- hsa-miR- MIMAT0000099
UACAGUACUGUGAUAACUGAA 456 101-1 101-1 101 65 GUACAGUACUGUGAUAACUGAA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000099 UACAGUACUGUGAUAACUGAA 457
101-2 101-2 101 66 AGCAGCAUUGUACAGGGCUAUGA hsa-mir- hsa-mir-
hsa-miR- MIMAT0000101 AGCAGCAUUGUACAGGGCUAUGA 458 103-1 103-1 103
67 AGCAGCAUUGUACAGGGCUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000101
AGCAGCAUUGUACAGGGCUAUGA 4589 103-2 103-2 103 68
AAAUGCUCAGACUCCUGUGGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000102
UCAAAUGCUCAGACUCCUGUGGU 460 105-1 105-1 105 69
AAAUGCUCAGACUCCUGUGGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000102
UCAAAUGCUCAGACUCCUGUGGU 461 105-2 105-2 105 70
AAAAGUGCUUACAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000103
AAAAGUGCUUACAGUGCAGGUAG 462 106a 106a 106a 71 UAAAGUGCUGACAGUGCAGAU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000680 UAAAGUGCUGACAGUGCAGAU 463
106b 106b 106b 72 AGCAGCAUUGUACAGGGCUAUCA hsa-mir- hsa-mir-
hsa-miR- MIMAT0000104 AGCAGCAUUGUACAGGGCUAUCA 464 107 107 107 73
UACCCUGUAGAUCCGAAUUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000253
UACCCUGUAGAUCCGAAUUUGUG 465 10a 10a 10a 74 UACCCUGUAGAACCGAAUUUGUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000254 UACCCUGUAGAACCGAAUUUGUG 466
10b 10b 10b 75 UGGAAUGUAAAGAAGUAUGUAU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000416 UGGAAUGUAAAGAAGUAUGUAU 467 1-1 1-1 1 76
UGGAAUGUAAAGAAGUAUGUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000416
UGGAAUGUAAAGAAGUAUGUAU 468 1-2 1-2 1 77 UGGAGUGUGACAAUGGUGUUUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000421 UGGAGUGUGACAAUGGUGUUUG 469
122 122 122 78 UAAGGCACGCGGUGAAUGCC hsa-mir- hsa-mir- hsa-miR-
MIMAT0000422 UAAGGCACGCGGUGAAUGCC 470 124-1 124-1 124 79
UAAGGCACGCGGUGAAUGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000422
UAAGGCACGCGGUGAAUGCC 471 124-2 124-2 124 80 UAAGGCACGCGGUGAAUGCC
hsa-mir- hsa-mir- hsa-miR- MIMAT0000422 UAAGGCACGCGGUGAAUGCC 472
124-3 124-3 124 81 ACCCGUCCCGUUCGUCCCCGGA hsa-mir- hsa-mir-
hsa-miR- MIMAT0005899 ACCCGUCCCGUUCGUCCCCGGA 473 1247 1247 1247 82
ACGGUGCUGGAUGUGGCCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0005902
ACGGUGCUGGAUGUGGCCUUU 474 1250 1250 1250 83 AGAAGGAAAUUGAAUUCAUUUA
hsa-mir- hsa-mir- hsa-miR- MIMAT0005944 AGAAGGAAAUUGAAUUCAUUUA 475
1252 1252 1252 84 AGCCUGGAAGCUGGAGCCUGCAGU hsa-mir- hsa-mir-
hsa-miR- MIMAT0005905 AGCCUGGAAGCUGGAGCCUGCAGU 476 1254 1254 1254
85 AGGAUGAGCAAAGAAAGUAGAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0005906
AGGAUGAGCAAAGAAAGUAGAUU 477 1255a 1255a 1255a 86
AGGCAUUGACUUCUCACUAGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005907
AGGCAUUGACUUCUCACUAGCU 478 1256 1256 1256 87 CGUACCGUGAGUAAUAAUGCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000445 UCGUACCGUGAGUAAUAAUGCG 479
126 126 126 88 AUGGGUGAAUUUGUAGAAGGAU hsa-mir- hsa-mir- hsa-miR-
MIMAT0005914 AUGGGUGAAUUUGUAGAAGGAU 480 1262 1262 1262 89
AUGGUACCCUGGCAUACUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005915
AUGGUACCCUGGCAUACUGAGU 481 1263 1263 1263 90 CAGGAUGUGGUCAAGUGUUGUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0005918 CAGGAUGUGGUCAAGUGUUGUU 482
1265 1265 1265 91 CCUCAGGGCUGUAGAACAGGGCU hsa-mir- hsa-mir-
hsa-miR- MIMAT0005920 CCUCAGGGCUGUAGAACAGGGCU 483 1266 1266 1266 92
CUGGACUGAGCCGUGCUACUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0005923
CUGGACUGAGCCGUGCUACUGG 484 1269 1269 1269 93
CUGGAGAUAUGGAAGAGCUGUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005924
CUGGAGAUAUGGAAGAGCUGUGU 485 1270 1270 1270 94
CUUGGCACCUAGCAAGCACUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0005796
CUUGGCACCUAGCAAGCACUCA 486 1271 1271 1271 95 UCGGAUCCGUCUGAGCUUGGCU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000446 UCGGAUCCGUCUGAGCUUGGCU 487
127-3p 127 127-3p 96 UACGUAGAUAUAUAUGUAUUUU hsa-mir- hsa-mir-
hsa-miR- MIMAT0005933 UACGUAGAUAUAUAUGUAUUUU 488 1277 1277 1277 97
UAGUACUGUGCAUAUCAUCUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005936
UAGUACUGUGCAUAUCAUCUAU 489 1278 1278 1278 98 UCACAGUGAACCGGUCUCUUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000424 UCACAGUGAACCGGUCUCUUU 490
128-1 128-1 128 99 UCACAGUGAACCGGUCUCUUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000424 UCACAGUGAACCGGUCUCUUU 491 128-2 128-2 128 100
CUUUUUGCGGUCUGGGCUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000242
CUUUUUGCGGUCUGGGCUUGC 492 129-1 129-1 129-5p 101
CUUUUUGCGGUCUGGGCUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000242
CUUUUUGCGGUCUGGGCUUGC 493 129-2 129-2 129-5p 102
UGUGAGGUUGGCAUUGUUGUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005884
UGUGAGGUUGGCAUUGUUGUCU 494 1294 1294 1294 103 UUAGGCCGCAGAUCUGGGUGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0005885 UUAGGCCGCAGAUCUGGGUGA 495
1295 1295 1295 104 UUCAUUCGGCUGUCCAGAUGUA hsa-mir- hsa-mir-
hsa-miR- MIMAT0005800 UUCAUUCGGCUGUCCAGAUGUA 496 1298 1298 1298 105
UUGCAGCUGCCUGGGAGUGACUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005797
UUGCAGCUGCCUGGGAGUGACUUC 497 1301 1301 1301 106
CGGUUUGAGGCUACAGUGAGAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005892
UUUGAGGCUACAGUGAGAUGUG 498 1304 1304 1304 107 ACGUUGGCUCUGGUGGUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0005950 ACGUUGGCUCUGGUGGUG 499 1306
1306 1306 108 ACUCGGCGUGGCGUCGGUCGUG hsa-mir- hsa-mir- hsa-miR-
MIMAT0005951 ACUCGGCGUGGCGUCGGUCGUG 500 1307 1307 1307 109
CAGUGCAAUGUUAAAAGGGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000425
CAGUGCAAUGUUAAAAGGGCAU 501 130a 130a 130a 110
CAGUGCAAUGAUGAAAGGGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000691
CAGUGCAAUGAUGAAAGGGCAU 502 130b 130b 130b 111
UAACAGUCUACAGCCAUGGUCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000426
UAACAGUCUACAGCCAUGGUCG 503 132 132 132 112 ACCGUGGCUUUCGAUUGUUACU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004594 ACCGUGGCUUUCGAUUGUUACU 504
132* 132 132* 113 UGUGACUGGUUGACCAGAGGGG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000447 UGUGACUGGUUGACCAGAGGGG 505 134 134 134 114
UAUGGCUUUUCAUUCCUAUGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000758
UAUGGCUUUUCAUUCCUAUGUGA 506 135b 135b 135b 115
ACUCCAUUUGUUUUGAUGAUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000448
ACUCCAUUUGUUUUGAUGAUGGA 507 136 136 136 116 AGCUGGUGUUGUGAAUCAGGCCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000430 AGCUGGUGUUGUGAAUCAGGCCG 508
138-1 138-1 138 117 AGCUGGUGUUGUGAAUCAGGCCG hsa-mir- hsa-mir-
hsa-miR- MIMAT0000430 AGCUGGUGUUGUGAAUCAGGCCG 509 138-2 138-2 138
118 UGGAGACGCGGCCCUGUUGGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004552
GGAGACGCGGCCCUGUUGGAGU 510 139-3p 139 139-3p 119
UACCACAGGGUAGAACCACGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004597
UACCACAGGGUAGAACCACGG 511 140-3p 140 140-3p 120
UAACACUGUCUGGUAAAGAUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000432
UAACACUGUCUGGUAAAGAUGG 512 141 141 141 121 CCCAUAAAGUAGAAAGCACU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000433 CAUAAAGUAGAAAGCACUACU 513
142 142 142-5p 122 UGAGAUGAAGCACUGUAGCUC hsa-mir- hsa-mir- hsa-miR-
MIMAT0000435 UGAGAUGAAGCACUGUAGCUC 514 143 143 143 123
GGAUAUCAUCAUAUACUGUAAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004600
GGAUAUCAUCAUAUACUGUAAG 515 144 144 144*
124 GUCCAGUUUUCCCAGGAAUCCCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000437
GUCCAGUUUUCCCAGGAAUCCCU 516 145 145 145 125 UGAGAACUGAAUUCCAUGGGUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000449 UGAGAACUGAAUUCCAUGGGUU 517
146a 146a 146a 126 UGAGAACUGAAUUCCAUAGGCU hsa-mir- hsa-mir-
hsa-miR- MIMAT0002809 UGAGAACUGAAUUCCAUAGGCU 518 146b 146b 146b-5p
127 UCAGUGCACUACAGAACUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000243
UCAGUGCACUACAGAACUUUGU 519 148a 148a 148a 128
UCAGUGCAUCACAGAACUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000759
UCAGUGCAUCACAGAACUUUGU 520 148b 148b 148b 129
UCUCCCAACCCUUGUACCAGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000451
UCUCCCAACCCUUGUACCAGUG 521 150 150 150 130 CUAGACUGAAGCUCCUUGAGG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000757 CUAGACUGAAGCUCCUUGAGG 522
151-3p 151 151-3p 131 UCAGUGCAUGACAGAACUUGG hsa-mir- hsa-mir-
hsa-miR- MIMAT0000438 UCAGUGCAUGACAGAACUUGG 523 152 152 152 132
UUAAUGCUAAUCGUGAUAGGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000646
UUAAUGCUAAUCGUGAUAGGGGU 524 155 155 155 133 UAGCAGCACAUAAUGGUUUGUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000068 UAGCAGCACAUAAUGGUUUGUG 525
15a 15a 15a 134 UAGCAGCACAUCAUGGUUUACA hsa-mir- hsa-mir- hsa-miR-
MIMAT0000417 UAGCAGCACAUCAUGGUUUACA 526 15b 15b 15b 135
UAGCAGCACGUAAAUAUUGGCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000069
UAGCAGCACGUAAAUAUUGGCG 527 16-1 16-1 16 136 UAGCAGCACGUAAAUAUUGGCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000069 UAGCAGCACGUAAAUAUUGGCG 528
16-2 16-2 16 137 CAAAGUGCUUACAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000070 CAAAGUGCUUACAGUGCAGGUAG 529 17 17 17 138
AACAUUCAACGCUGUCGGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000256
AACAUUCAACGCUGUCGGUGAGU 530 181a-1 181a-1 181a 139
AACAUUCAACGCUGUCGGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000256
AACAUUCAACGCUGUCGGUGAGU 531 181a-2 181a-2 181a 140
AACAUUCAUUGCUGUCGGUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000257
AACAUUCAUUGCUGUCGGUGGGU 532 181b-1 181b-1 181b 141
AACAUUCAUUGCUGUCGGUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000257
AACAUUCAUUGCUGUCGGUGGGU 533 181b-2 181b-2 181b 142
AACAUUCAACCUGUCGGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000258
AACAUUCAACCUGUCGGUGAGU 534 181c 181c 181c 143
AACAUUCAUUGUUGUCGGUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0002821
AACAUUCAUUGUUGUCGGUGGGU 535 181d 181d 181d 144
UUUGGCAAUGGUAGAACUCACACU hsa-mir- hsa-mir- hsa-miR- MIMAT0000259
UUUGGCAAUGGUAGAACUCACACU 536 182 182 182 145 UAUGGCACUGGUAGAAUUCACU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000261 UAUGGCACUGGUAGAAUUCACU 537
183 183 183 146 UGGACGGAGAACUGAUAAGGGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000454 UGGACGGAGAACUGAUAAGGGU 538 184 184 184 147
UGGAGAGAAAGGCAGUUCCUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000455
UGGAGAGAAAGGCAGUUCCUGA 539 185 185 185 148 CAAAGAAUUCUCCUUUUGGGCU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000456 CAAAGAAUUCUCCUUUUGGGCU 540
186 186 186 149 CAUCCCUUGCAUGGUGGAGGG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000457 CAUCCCUUGCAUGGUGGAGGG 541 188-5p 188 188-5p 150
UAAGGUGCAUCUAGUGCAGAUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000072
UAAGGUGCAUCUAGUGCAGAUAG 542 18a 18a 18a 151 CAACGGAAUCCCAAAAGCAGCUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000440 CAACGGAAUCCCAAAAGCAGCUG 543
191 191 191 152 CUGACCUAUGAAUUGACAGCC hsa-mir- hsa-mir- hsa-miR-
MIMAT0000222 CUGACCUAUGAAUUGACAGCC 544 192 192 192 153
UGGGUCUUUGCGGGCGAGAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004614
UGGGUCUUUGCGGGCGAGAUGA 545 193a-5p 193a 193a-5p 154
AACUGGCCCUCAAAGUCCCGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0002819
AACUGGCCCUCAAAGUCCCGCU 546 193b 193b 193b 155
UGUAACAGCAACUCCAUGUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000460
UGUAACAGCAACUCCAUGUGGA 547 194-1 194-1 194 156
UAGCAGCACAGAAAUAUUGGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000461
UAGCAGCACAGAAAUAUUGGC 548 195 195 195 157 UAGGUAGUUUCAUGUUGUUGGG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000226 UAGGUAGUUUCAUGUUGUUGGG 549
196a-1 196a-1 196a 158 UAGGUAGUUUCAUGUUGUUGGG hsa-mir- hsa-mir-
hsa-miR- MIMAT0000226 UAGGUAGUUUCAUGUUGUUGGG 550 196a-2 196a-2 196a
159 UAGGUAGUUUCCUGUUGUUGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0001080
UAGGUAGUUUCCUGUUGUUGGG 551 196b 196b 196b 160
UUCACCACCUUCUCCACCCAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000227
UUCACCACCUUCUCCACCCAGC 552 197 197 197 161 ACAGUAGUCUGCACAUUGGUUA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000232 ACAGUAGUCUGCACAUUGGUUA 553
199a-1 199a-1 199a-3p 162 ACAGUAGUCUGCACAUUGGUUA hsa-mir- hsa-mir-
hsa-miR- MIMAT0000232 ACAGUAGUCUGCACAUUGGUUA 554 199a-2 199a-2
199a-3p 163 ACAGUAGUCUGCACAUUGGUUA hsa-mir- hsa-mir- hsa-miR-
MIMAT0004563 ACAGUAGUCUGCACAUUGGUUA 555 199b 199b 199b-3p 164
UGUGCAAAUCUAUGCAAAACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000073
UGUGCAAAUCUAUGCAAAACUGA 556 19a 19a 19a 165 UGUGCAAAUCCAUGCAAAACUGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000074 UGUGCAAAUCCAUGCAAAACUGA 557
19b-1 19b-1 19b 166 UGUGCAAAUCCAUGCAAAACUGA hsa-mir- hsa-mir-
hsa-miR- MIMAT0000074 UGUGCAAAUCCAUGCAAAACUGA 558 19b-2 19b-2 19b
167 UAACACUGUCUGGUAACGAUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000682
UAACACUGUCUGGUAACGAUGU 559 200a 200a 200a 168
UAAUACUGCCUGGUAAUGAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000318
UAAUACUGCCUGGUAAUGAUGA 560 200b 200b 200b 169
UAAUACUGCCGGGUAAUGAUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000617
UAAUACUGCCGGGUAAUGAUGGA 561 200c 200c 200c 170
UUCCUAUGCAUAUACUUCUUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0002810
UUCCUAUGCAUAUACUUCUUUG 562 202* 202 202* 171 GUGAAAUGUUUAGGACCACUAG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000264 GUGAAAUGUUUAGGACCACUAG 563
203 203 203 172 UUCCCUUUGUCAUCCUAUGCCU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000265 UUCCCUUUGUCAUCCUAUGCCU 564 204 204 204 173
UCCUUCAUUCCACCGGAGUCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000266
UCCUUCAUUCCACCGGAGUCUG 565 205 205 205 174 UGGAAUGUAAGGAAGUGUGUGG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000462 UGGAAUGUAAGGAAGUGUGUGG 566
206 206 206 175 UAAAGUGCUUAUAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000075 UAAAGUGCUUAUAGUGCAGGUAG 567 20a 20a 20a 176
CAAAGUGCUCAUAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0001413
CAAAGUGCUCAUAGUGCAGGUAG 568 20b 20b 20b 177 UAGCUUAUCAGACUGAUGUUGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000076 UAGCUUAUCAGACUGAUGUUGA 569
21 21 21 178 CUGUGCGUGUGACAGCGGCUGA hsa-mir- hsa-mir- hsa-miR-
MIMAT0000267 CUGUGCGUGUGACAGCGGCUGA 570 210 210 210 179
UAACAGUCUCCAGUCACGGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000269
UAACAGUCUCCAGUCACGGCC 571 212 212 212 180 ACAGCAGGCACAGACAGGCAGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000271 ACAGCAGGCACAGACAGGCAGU 572
214 214 214 181 UGACCUAUGAAUUGACAG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000272 AUGACCUAUGAAUUGACAGAC 573 215 215 215 182
AAAUCUCUGCAGGCAAAUGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004959
AAAUCUCUGCAGGCAAAUGUGA 574 216b 216b 216b 183
AUACUGCAUCAGGAACUGAUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000274
UACUGCAUCAGGAACUGAUUGGA 575 217 217 217 184 AGAGUUGAGUCUGGACGUCCCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0004567 AGAGUUGAGUCUGGACGUCCCG 576
219-1 219-1 219-1-3p 185 AAGCUGCCAGUUGAAGAACUGU hsa-mir- hsa-mir-
hsa-miR- MIMAT0000077 AAGCUGCCAGUUGAAGAACUGU 577 22 22 22 186
AGCUACAUUGUCUGCUGGGUUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000278
AGCUACAUUGUCUGCUGGGUUUC 578 221 221 221
187 AGCUACAUCUGGCUACUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000279
AGCUACAUCUGGCUACUGGGU 579 222 222 222 188 CGUGUAUUUGACAAGCUGAGUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004570 CGUGUAUUUGACAAGCUGAGUU 580
223* 223 223* 189 CAAGUCACUAGUGGUUCCGUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000281 CAAGUCACUAGUGGUUCCGUU 581 224 224 224 190
AUCACAUUGCCAGGGAUUUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000078
AUCACAUUGCCAGGGAUUUCC 582 23a 23a 23a 191 AUCACAUUGCCAGGGAUUACC
hsa-mir- hsa-mir- hsa-miR- MIMAT0000418 AUCACAUUGCCAGGGAUUACC 583
23b 23b 23b 192 UGGCUCAGUUCAGCAGGAACAG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000080 UGGCUCAGUUCAGCAGGAACAG 584 24-1 24-1 24 193
UGGCUCAGUUCAGCAGGAACAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000080
UGGCUCAGUUCAGCAGGAACAG 585 24-2 24-2 24 194 CAUUGCACUUGUCUCGGUCUGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000081 CAUUGCACUUGUCUCGGUCUGA 586
25 25 25 195 UUCAAGUAAUCCAGGAUAGGCU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000082 UUCAAGUAAUCCAGGAUAGGCU 587 26a-1 26a-1 26a 196
UUCAAGUAAUCCAGGAUAGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000082
UUCAAGUAAUCCAGGAUAGGCU 588 26a-2 26a-2 26a 197
UUCAAGUAAUUCAGGAUAGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000083
UUCAAGUAAUUCAGGAUAGGU 589 26b 26b 26b 198 UUCACAGUGGCUAAGUUCCGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0000084 UUCACAGUGGCUAAGUUCCGC 590
27a 27a 27a 199 UUCACAGUGGCUAAGUUCUGC hsa-mir- hsa-mir- hsa-miR-
MIMAT0000419 UUCACAGUGGCUAAGUUCUGC 591 27b 27b 27b 200
CACUAGAUUGUGAGCUCCUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004502
CACUAGAUUGUGAGCUCCUGGA 592 28 28 28-3p 201 GAGGGUUGGGUGGAGGCUCUCC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004679 GAGGGUUGGGUGGAGGCUCUCC 593
296-3p 296 296-3p 202 AUGGUUUACCGUCCCACAUACA hsa-mir- hsa-mir-
hsa-miR- MIMAT0002890 UGGUUUACCGUCCCACAUACAU 594 299-5p 299 299-5p
203 UAGCACCAUCUGAAAUCGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000086
UAGCACCAUCUGAAAUCGGUUA 595 29a 29a 29a 204 UAGCACCAUUUGAAAUCAGUGUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000100 UAGCACCAUUUGAAAUCAGUGUU 596
29b-1 29b-1 29b 205 UAGCACCAUUUGAAAUCAGUGUU hsa-mir- hsa-mir-
hsa-miR- MIMAT0000100 UAGCACCAUUUGAAAUCAGUGUU 597 29b-2 29b-2 29b
206 UAGCACCAUUUGAAAUCGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000681
UAGCACCAUUUGAAAUCGGUUA 598 29c 29c 29c 207 CAGUGCAAUGAUAUUGUCAAAGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004958 CAGUGCAAUGAUAUUGUCAAAGC 599
301b 301b 301b 208 UGUAAACAUCCUCGACUGGAAG hsa-mir- hsa-mir-
hsa-miR- MIMAT0000087 UGUAAACAUCCUCGACUGGAAG 600 30a 30a 30a 209
UGUAAACAUCCUACACUCAGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000420
UGUAAACAUCCUACACUCAGCU 601 30b 30b 30b 210 UGUAAACAUCCUACACUCUCAGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0000244 UGUAAACAUCCUACACUCUCAGC 602
30c-1 30c-1 30c 211 UGUAAACAUCCUACACUCUCAGC hsa-mir- hsa-mir-
hsa-miR- MIMAT0000244 UGUAAACAUCCUACACUCUCAGC 603 30c-2 30c-2 30c
212 UGUAAACAUCCCCGACUGGAAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000245
UGUAAACAUCCCCGACUGGAAG 604 30d 30d 30d 213 UGUAAACAUCCUUGACUGGAAG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000692 UGUAAACAUCCUUGACUGGAAG 605
30e 30e 30e 214 AGGCAAGAUGCUGGCAUAGCU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000089 AGGCAAGAUGCUGGCAUAGCU 606 31 31 31 215
UAUUGCACAUUACUAAGUUGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000090
UAUUGCACAUUACUAAGUUGCA 607 32 32 32 216 AAAAGCUGGGUUGAGAGGGCGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000510 AAAAGCUGGGUUGAGAGGGCGA 608
320a 320a 320a 217 AAAAGCUGGGUUGAGAGGGCAA hsa-mir- hsa-mir-
hsa-miR- MIMAT0005792 AAAAGCUGGGUUGAGAGGGCAA 609 320b-1 320b-1 320b
218 AAAAGCUGGGUUGAGAGGGCAA hsa-mir- hsa-mir- hsa-miR- MIMAT0005792
AAAAGCUGGGUUGAGAGGGCAA 610 320b-2 320b-2 320b 219
AAAAGCUGGGUUGAGAGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005793
AAAAGCUGGGUUGAGAGGGU 611 320c-1 320c-1 320c 220
AAAAGCUGGGUUGAGAGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005793
AAAAGCUGGGUUGAGAGGGU 612 320c-2 320c-2 320c 221 AAAAGCUGGGUUGAGAGGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0006764 AAAAGCUGGGUUGAGAGGA 613
320d-1 320d-1 320d 222 AAAAGCUGGGUUGAGAGGA hsa-mir- hsa-mir-
hsa-miR- MIMAT0006764 AAAAGCUGGGUUGAGAGGA 614 320d-2 320d-2 320d
223 CACAUUACACGGUCGACCUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000755
CACAUUACACGGUCGACCUCU 615 323 323 323-3p 224
CGCAUCCCCUAGGGCAUUGGUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000761
CGCAUCCCCUAGGGCAUUGGUGU 616 324 324 5324-p 225
CUGGCCCUCUCUGCCCUUCCGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000752
CUGGCCCUCUCUGCCCUUCCGU 617 328 328 328 226 GCAAAGCACACGGCCUGCAGAGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000751 GCAAAGCACACGGCCUGCAGAGA 618
330 330 330-3p 227 GCCCCUGGGCCUAUCCUAGAA hsa-mir- hsa-mir- hsa-miR-
MIMAT0000760 GCCCCUGGGCCUAUCCUAGAA 619 331 331 331-3p 228
UCAAGAGCAAUAACGAAAAAUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000765
UCAAGAGCAAUAACGAAAAAUGU 620 335 335 335 229 UCCAGCAUCAGUGAUUUUGUUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000763 UCCAGCAUCAGUGAUUUUGUUG 621
338 338 338-3p 230 UGAGCGCCUCGACGACAGAGCCG hsa-mir- hsa-mir-
hsa-miR- MIMAT0004702 UGAGCGCCUCGACGACAGAGCCG 622 339-3p 339 339-3p
231 GUGCAUUGUAGUUGCAUUGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000091
GUGCAUUGUAGUUGCAUUGCA 623 33a 33a 33a 232 GUGCAUUGCUGUUGCAUUGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0003301 GUGCAUUGCUGUUGCAUUGC 624
33b 33b 33b 233 UUAUAAAGCAAUGAGACUGAUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0004692 UUAUAAAGCAAUGAGACUGAUU 625 340 340 340 234
AGGGGUGCUAUCUGUGAUUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004694
AGGGGUGCUAUCUGUGAUUGA 626 342-5p 342 342-5p 235
GCUGACUCCUAGUCCAGGGCUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000772
GCUGACUCCUAGUCCAGGGCUC 627 345 345 345 236 UGGCAGUGUCUUAGCUGGUUGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000255 UGGCAGUGUCUUAGCUGGUUGU 628
34a 34a 34a 237 AGGCAGUGUCAUUAGCUGAUUG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000685 UAGGCAGUGUCAUUAGCUGAUUG 629 34b* 34b 34b* 238
AGGCAGUGUAGUUAGCUGAUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000686
AGGCAGUGUAGUUAGCUGAUUGC 630 34c-5p 34c 34c-5p 239
UUAUCAGAAUCUCCAGGGGUAC hsa-mir- hsa-mir- hsa-miR- MIMAT0000703
UUAUCAGAAUCUCCAGGGGUAC 631 361-5p 361 361-5p 240
AACACACCUAUUCAAGGAUUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0004683
AACACACCUAUUCAAGGAUUCA 632 362-3p 362 362-3p 241
AAUUGCACGGUAUCCAUCUGUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000707
AAUUGCACGGUAUCCAUCUGUA 633 363 363 363 242 UAAUGCCCCUAAAAAUCCUUAU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000710 UAAUGCCCCUAAAAAUCCUUAU 634
365-2 365-2 365 243 AAUAAUACAUGGUUGAUCUUU hsa-mir- hsa-mir-
hsa-miR- MIMAT0000721 AAUAAUACAUGGUUGAUCUUU 635 369 369 369-3p 244
GCCUGCUGGGGUGGAACCUGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000722
GCCUGCUGGGGUGGAACCUGGU 636 370 370 370 245 ACUCAAACUGUGGGGGCACU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004687 ACUCAAACUGUGGGGGCACU 637
371 371 371-5p 246 UUAUAAUACAACCUGAUAAGUG hsa-mir- hsa-mir-
hsa-miR- MIMAT0000727 UUAUAAUACAACCUGAUAAGUG 638 374a 374a 374a 247
AUAUAAUACAACCUGCUAAGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004955
AUAUAAUACAACCUGCUAAGUG 639 374b 374b 374b 248
UUUGUUCGUUCGGCUCGCGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000728
UUUGUUCGUUCGGCUCGCGUGA 640 375 375 375 249 AGAGGUUGCCCUUGGUGAAUUC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004689 AGAGGUUGCCCUUGGUGAAUUC
641
377* 377 377* 250 ACUGGACUUGGAGUCAGAAGG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000732 ACUGGACUUGGAGUCAGAAGG 642 378 378 378 251
UGGUAGACUAUGGAACGUAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000733
UGGUAGACUAUGGAACGUAGG 643 379 379 379 252 UAUACAAGGGCAAGCUCUCUGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000736 UAUACAAGGGCAAGCUCUCUGU 644
381 381 381 253 GAAGUUGUUCGUGGUGGAUUCG hsa-mir- hsa-mir- hsa-miR-
MIMAT0000737 GAAGUUGUUCGUGGUGGAUUCG 645 382 382 382 254
AGAUCAGAAGGUGAUUGUGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000738
AGAUCAGAAGGUGAUUGUGGCU 646 383 383 383 255 CGAAUGUUGCUCGGUGAACCCC
hsa-mir- hsa-mir- hsa-miR- MIMAT0001639 GAAUGUUGCUCGGUGAACCCCU 647
409-3p 409 409-3p 256 AAUAUAACACAGAUGGCCUGU hsa-mir- hsa-mir-
hsa-miR- MIMAT0002171 AAUAUAACACAGAUGGCCUGU 648 410 410 410 257
AUAGUAGACCGUAUAGCGUACG hsa-mir- hsa-mir- hsa-miR- MIMAT0003329
UAGUAGACCGUAUAGCGUACG 649 411 411 411 258 AUCAACAGACAUUAAUUGGGCGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0003339 AUCAACAGACAUUAAUUGGGCGC 650
421 421 421 259 UGAGGGGCAGAGAGCGAGACUUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0004748 UGAGGGGCAGAGAGCGAGACUUU 651 423 423 423-5p 260
CAGCAGCAAUUCAUGUUUUGAA hsa-mir- hsa-mir- hsa-miR- MIMAT0001341
CAGCAGCAAUUCAUGUUUUGAA 652 424 424 424 261 UAAUACUGUCUGGUAAAACCGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0001536 UAAUACUGUCUGGUAAAACCGU 653
429 429 429 262 UCUUGGAGUAGGUCAUUGGGUGG hsa-mir- hsa-mir- hsa-miR-
MIMAT0002814 UCUUGGAGUAGGUCAUUGGGUGG 654 432 432 432 263
AUCAUGAUGGGCUCCUCGGUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0001627
AUCAUGAUGGGCUCCUCGGUGU 655 433 433 433 264 UUGCAUAUGUAGGAUGUCCCAU
hsa-mir- hsa-mir- hsa-miR- MIMAT0001532 UUGCAUAUGUAGGAUGUCCCAU 656
448 448 448 265 UGGCAGUGUAUUGUUAGCUGGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0001541 UGGCAGUGUAUUGUUAGCUGGU 657 449a 449a 449a 266
AGGCAGUGUAUUGUUAGCUGGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003327
AGGCAGUGUAUUGUUAGCUGGC 658 449b 449b 449b 267
UUUUGCGAUGUGUUCCUAAUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001545
UUUUGCGAUGUGUUCCUAAUAU 659 450a-1 450a-1 450a 268
UUUUGCGAUGUGUUCCUAAUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001545
UUUUGCGAUGUGUUCCUAAUAU 660 450a-2 450a-2 450a 269
UUUUGCAAUAUGUUCCUGAAUA hsa-mir- hsa-mir- hsa-miR- MIMAT0004909
UUUUGCAAUAUGUUCCUGAAUA 661 450b-5p 450b 450b-5p 270
AACUGUUUGCAGAGGAAACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0001635
AACUGUUUGCAGAGGAAACUGA 662 452 452 452 271 UAGUGCAAUAUUGCUUAUAGGGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0003885 UAGUGCAAUAUUGCUUAUAGGGU 663
454 454 454 272 GCAGUCCAUGGGCAUAUACAC hsa-mir- hsa-mir- hsa-miR-
MIMAT0004784 GCAGUCCAUGGGCAUAUACAC 664 455-3p 455 455-3p 273
AAGACGGGAGGAAAGAAGGGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004761
AAGACGGGAGGAAAGAAGGGAG 665 483-5p 483 483-5p 274
UCAGGCUCAGUCCCCUCCCGAU hsa-mir- hsa-mir- hsa-miR- MIMAT0002174
UCAGGCUCAGUCCCCUCCCGAU 666 484 484 484 275 AGAGGCUGGCCGUGAUGAAUUC
hsa-mir- hsa-mir- hsa-miR- MIMAT0002175 AGAGGCUGGCCGUGAUGAAUUC 667
485-5p 485 485-5p 276 UCCUGUACUGAGCUGCCCCGAG hsa-mir- hsa-mir-
hsa-miR- MIMAT0002177 UCCUGUACUGAGCUGCCCCGAG 668 486-5p 486 486-5p
277 AAUCGUACAGGGUCAUCCACUU hsa-mir- hsa-mir- hsa-miR- MIMAT0003180
AAUCGUACAGGGUCAUCCACUU 669 487b 487b 487b 278 CCCAGAUAAUGGCACUCUCAA
hsa-mir- hsa-mir- hsa-miR- MIMAT0002804 CCCAGAUAAUGGCACUCUCAA 670
488* 488 488* 279 UUGUACAUGGUAGGCUUUCAUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0002813 UUGUACAUGGUAGGCUUUCAUU 671 493* 493 493* 280
UGAAACAUACACGGGAAACCUC hsa-mir- hsa-mir- hsa-miR- MIMAT0002816
UGAAACAUACACGGGAAACCUC 672 494 494 494 281 AAACAAACAUGGUGCACUUCUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0002817 AAACAAACAUGGUGCACUUCUU 673
495 495 495 282 CAGCAGCACACUGUGGUUUGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0002820 CAGCAGCACACUGUGGUUUGU 674 497 497 497 283
UUAAGACUUGCAGUGAUGUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0002870
UUAAGACUUGCAGUGAUGUUU 675 499-5p 499 499-5p 284
AUGCACCUGGGCAAGGAUUCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0002871
AUGCACCUGGGCAAGGAUUCUG 676 500* 500 500* 285 AAUGCACCCGGGCAAGGAUUCU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004774 AAUGCACCCGGGCAAGGAUUCU 677
501-3p 501 501-3p 286 AAUGCACCUGGGCAAGGAUUCA hsa-mir- hsa-mir-
hsa-miR- MIMAT0004775 AAUGCACCUGGGCAAGGAUUCA 678 502-3p 502 502-3p
287 UAGCAGCGGGAACAGUUCUGCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0002874
UAGCAGCGGGAACAGUUCUGCAG 679 503 503 503 288 AGACCCUGGUCUGCACUCUAUC
hsa-mir- hsa-mir- hsa-miR- MIMAT0002875 AGACCCUGGUCUGCACUCUAUC 680
504 504 504 289 GGGAGCCAGGAAGUAUUGAUGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0004776 GGGAGCCAGGAAGUAUUGAUGU 681 505* 505 505* 290
UGAUUGUAGCCUUUUGGAGUAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0002880
UGAUUGUAGCCUUUUGGAGUAGA 682 508-3p 508 508-3p 291
UACUGCAGACGUGGCAAUCAUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004975
UACUGCAGACGUGGCAAUCAUG 683 509-3-5p 509-3 509-3-5p 292
UUCACAGGGAGGUGUCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0002877
UUCACAGGGAGGUGUCAU 684 513a-1 513a-1 513a-5p 293 UUCACAGGGAGGUGUCAU
hsa-mir- hsa-mir- hsa-miR- MIMAT0002877 UUCACAGGGAGGUGUCAU 685
513a-2 513a-2 513a-5p 294 UUCACAAGGAGGUGUCAUUUAU hsa-mir- hsa-mir-
hsa-miR- MIMAT0005788 UUCACAAGGAGGUGUCAUUUAU 686 513b 513b 513b 295
UUCUCAAGGAGGUGUCGUUUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005789
UUCUCAAGGAGGUGUCGUUUAU 687 513c 513c 513c 296
CAUGCCUUGAGUGUAGGACCGU hsa-mir- hsa-mir- hsa-miR- MIMAT0002888
CAUGCCUUGAGUGUAGGACCGU 688 532 532 532-5p 297
UGUGACAGAUUGAUAACUGAAA hsa-mir- hsa-mir- hsa-miR- MIMAT0003389
UGUGACAGAUUGAUAACUGAAA 689 542-3p 542 542-3p 298
AAACAUUCGCGGUGCACUUCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004954
AAACAUUCGCGGUGCACUUCUU 690 543 543 543 299 CAAAACUGGCAAUUACUUUUGC
hsa-mir- hsa-mir- hsa-miR- MIMAT0003251 CAAAACUGGCAAUUACUUUUGC 691
548a-1 548a-1 548a-3p 300 CAAAACUGGCAAUUACUUUUGC hsa-mir- hsa-mir-
hsa-miR- MIMAT0003251 CAAAACUGGCAAUUACUUUUGC 692 548a-2 548a-2
548a-3p 301 CAAAACUGGCAAUUACUUUUGC hsa-mir- hsa-mir- hsa-miR-
MIMAT0003251 CAAAACUGGCAAUUACUUUUGC 693 548a-3 548a-3 548a-3p 302
CAAGAACCUCAGUUGCUUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0003254
CAAGAACCUCAGUUGCUUUUGU 694 548b-3p 548b 548b-3p 303
AAAAACUGAGACUACUUUUGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0005874
AAAAACUGAGACUACUUUUGCA 695 548e 548e 548e 304
AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928
AAAAGUAAUCGCGGUUUUUGUC 696 548h-1 548h-1 548h 305
AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928
AAAAGUAAUCGCGGUUUUUGUC 697 548h-2 548h-2 548h 306
AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928
AAAAGUAAUCGCGGUUUUUGUC 698 548h-3 548h-3 548h 307
AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928
AAAAGUAAUCGCGGUUUUUGUC 699 548h-4 548h-4 548h 308
AAAAGUAAUUGCGGUCUUUGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005875
AAAAGUAAUUGCGGUCUUUGGU 700 548j 548j 548j 309
AAAAGUACUUGCGGAUUUUGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005882
AAAAGUACUUGCGGAUUUUGCU 701 548k 548k 548k 310
AAAAGUAUUUGCGGGUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005889
AAAAGUAUUUGCGGGUUUUGUC 702 548l 548l 548l 311
CAAAAGUAAUUGUGGAUUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005916
CAAAAGUAAUUGUGGAUUUUGU 703 548n 548n 548n 312
AGUGCCUGAGGGAGUAAGAGCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004800
AGUGCCUGAGGGAGUAAGAGCCC 704 550-1 550-1 550 313
AGUGCCUGAGGGAGUAAGAGCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004800
AGUGCCUGAGGGAGUAAGAGCCC 705 550-2 550-2 550 314
GCGACCCAUACUUGGUUUCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003233
GCGACCCAUACUUGGUUUCAG 706 551b 551b 551b 315 CACGCUCAUGCACACACCCACA
hsa-mir- hsa-mir- hsa-miR- MIMAT0003239 CACGCUCAUGCACACACCCACA 707
574-3p 574 574-3p 316 AAGAUGUGGAAAAAUUGGAAUC hsa-mir- hsa-mir-
hsa-miR- MIMAT0004796 AAGAUGUGGAAAAAUUGGAAUC 708 576-3p 576 576-3p
317 GUAGAUAAAAUAUUGGUACCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003242
UAGAUAAAAUAUUGGUACCUG 709 577 577 577 318 UAACUGGUUGAACAACUGAACC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004797 UAACUGGUUGAACAACUGAACC 710
582-3p 582 582-3p 319 UUACAGUUGUUCAACCAGUUACU hsa-mir- hsa-mir-
hsa-miR- MIMAT0003247 UUACAGUUGUUCAACCAGUUACU 711 582-5p 582 582-5p
320 UUAUGGUUUGCCUGGGACUGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003249
UUAUGGUUUGCCUGGGACUGAG 712 584 584 584 321 UGAGAACCACGUCUGCUCUGAG
hsa-mir- hsa-mir- hsa-miR- MIMAT0004799 UGAGAACCACGUCUGCUCUGAG 713
589 589 589 322 UAAUUUUAUGUAUAAGCUAGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0004801 UAAUUUUAUGUAUAAGCUAGU 714 590-3p 590 590-3p 323
UACGUCAUCGUUGUCAUCGUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0003266
UACGUCAUCGUUGUCAUCGUCA 715 598 598 598 324 UCCGAGCCUGGGUCUCCCUCUU
hsa-mir- hsa-mir- hsa-miR- MIMAT0003283 UCCGAGCCUGGGUCUCCCUCUU 716
615-3p 615 615-3p 325 AAGUCAUUGGAGGGUUUGAGCA hsa-mir- hsa-mir-
hsa-miR- MIMAT0004805 AGUCAUUGGAGGGUUUGAGCAG 717 616 616 616 326
AAACUCUACUUGUCCUUCUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0003287
AAACUCUACUUGUCCUUCUGAGU 718 618 618 618 327 AGGGGGAAAGUUCUAUAGUCC
hsa-mir- hsa-mir- hsa-miR- MIMAT0003294 AGGGGGAAAGUUCUAUAGUCC 719
625 625 625 328 AUGCUGACAUAUUUACUAGAGG hsa-mir- hsa-mir- hsa-miR-
MIMAT0004809 AUGCUGACAUAUUUACUAGAGG 720 628-5p 628 628-5p 329
UGGGUUUACGUUGGGAGAACU hsa-mir- hsa-mir- hsa-miR- MIMAT0004810
UGGGUUUACGUUGGGAGAACU 721 629 629 629 330 AAAGACAUAGGAUAGAGUCACCUC
hsa-mir- hsa-mir- hsa-miR- MIMAT0003311 AAAGACAUAGGAUAGAGUCACCUC
722 641 641 641 331 ACACUUGUAUGCUAGCUCAGGU hsa-mir- hsa-mir-
hsa-miR- MIMAT0003313 ACUUGUAUGCUAGCUCAGGUAG 723 643 643 643 332
UUUAGGAUAAGCUUGACUUUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003321
UUUAGGAUAAGCUUGACUUUUG 724 651 651 651 333 AAUGGCGCCACUAGGGUUGUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0003322 AAUGGCGCCACUAGGGUUGUG 725
652 652 652 334 UAUGUCUGCUGACCAUCACCUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0004814 UAUGUCUGCUGACCAUCACCUU 726 654-3p 654 654-3p 335
UACCCAUUGCAUAUCGGAGUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003338
UACCCAUUGCAUAUCGGAGUUG 727 660 660 660 336 UCCGGUUCUCAGGGCUCCACC
hsa-mir- hsa-mir- hsa-miR- MIMAT0004819 UCCGGUUCUCAGGGCUCCACC 728
671-3p 671 671-3p 337 AAGGAGCUUACAAUCUAGCUGGG hsa-mir- hsa-mir-
hsa-miR- MIMAT0004926 AAGGAGCUUACAAUCUAGCUGGG 729 708 708 708 338
UGGAAGACUAGUGAUUUUGUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000252
UGGAAGACUAGUGAUUUUGUUGU 730 7-1 7-1 7 339 UGGAAGACUAGUGAUUUUGUUGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000252 UGGAAGACUAGUGAUUUUGUUGU 731
7-2 7-2 7 340 UGGAAGACUAGUGAUUUUGUUGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000252 UGGAAGACUAGUGAUUUUGUUGU 732 7-3 7-3 7 341
CGGCUCUGGGUCUGUGGGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004957
CGGCUCUGGGUCUGUGGGGA 733 760 760 760 342 UGCACCAUGGUUGUCUGAGCAUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0003882 UGCACCAUGGUUGUCUGAGCAUG 734
767-5p 767 767-5p 343 UGAGACCUCUGGGUUCUGAGCU hsa-mir- hsa-mir-
hsa-miR- MIMAT0003886 UGAGACCUCUGGGUUCUGAGCU 735 769-5p 769 769-5p
344 GCAGGAACUUGUGAGUCUCCU hsa-mir- hsa-mir- hsa-miR- MIMAT0004953
GCAGGAACUUGUGAGUCUCCU 736 873 873 873 345 CUGCCCUGGCCCGAGGGACCGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0004911 CUGCCCUGGCCCGAGGGACCGA 737
874 874 874 346 GUAGAGGAGAUGGCGCAGGG hsa-mir- hsa-mir- hsa-miR-
MIMAT0004949 GUAGAGGAGAUGGCGCAGGG 738 877 877 877 347
GUGAACGGGCGCCAUCCCGAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004951
GUGAACGGGCGCCAUCCCGAGG 739 887 887 887 348 UUAAUAUCGGACAACCAUUGU
hsa-mir- hsa-mir- hsa-miR- MIMAT0004921 UUAAUAUCGGACAACCAUUGU 740
889 889 889 349 UGCAACGAACCUGAGCCACUGA hsa-mir- hsa-mir- hsa-miR-
MIMAT0004902 UGCAACGAACCUGAGCCACUGA 741 891a 891a 891a 350
CACUGUGUCCUUUCUGCGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004907
CACUGUGUCCUUUCUGCGUAG 742 892a 892a 892a 351
UCUUUGGUUAUCUAGCUGUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000441
UCUUUGGUUAUCUAGCUGUAUGA 743 9-1 9-1 9 352 UCUUUGGUUAUCUAGCUGUAUGA
hsa-mir- hsa-mir- hsa-miR- MIMAT0000441 UCUUUGGUUAUCUAGCUGUAUGA 744
9-2 9-2 9 353 UAUUGCACUUGUCCCGGCCUGU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000092 UAUUGCACUUGUCCCGGCCUGU 745 92a-1 92a-1 92a 354
UAUUGCACUUGUCCCGGCCUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000092
UAUUGCACUUGUCCCGGCCUGU 746 92a-2 92a-2 92a 355
UAUUGCACUCGUCCCGGCCUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0003218
UAUUGCACUCGUCCCGGCCUCC 747 92b 92b 92b 356 CAAAGUGCUGUUCGUGCAGGUAG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000093 CAAAGUGCUGUUCGUGCAGGUAG 748
93 93 93 357 UCUUUGGUUAUCUAGCUGUAUGA hsa-mir- hsa-mir- hsa-miR-
MIMAT0000441 UCUUUGGUUAUCUAGCUGUAUGA 749 9-3 9-3 9 358
AAGGCAGGGCCCCCGCUCCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004983
AAGGCAGGGCCCCCGCUCCCC 750 940 940 940 359 UUCUCUGUUUUGGCCAUGUGUG
hsa-mir- hsa-mir- hsa-miR- MIMAT0004985 UCUUCUCUGUUUUGGCCAUGUG 751
942 942 942 360 AAAUUAUUGUACAUCGGAUGAG hsa-mir- hsa-mir- hsa-miR-
MIMAT0004987 AAAUUAUUGUACAUCGGAUGAG 752 944 944 944 361
UUCAACGGGUAUUUAUUGAGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000094
UUCAACGGGUAUUUAUUGAGCA 753 95 95 95 362 UUUGGCACUAGCACAUUUUUGCU
hsa-mir- hsa-mir- hsa-miR- MIMAT0000095 UUUGGCACUAGCACAUUUUUGCU 754
96 96 96 363 UGAGGUAGUAAGUUGUAUUGUU hsa-mir- hsa-mir- hsa-miR-
MIMAT0000096 UGAGGUAGUAAGUUGUAUUGUU 755 98 98 98 364
AACCCGUAGAUCCGAUCUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000097
AACCCGUAGAUCCGAUCUUGUG 756 99a 99a 99a 365 CACCCGUAGAACCGACCUUGCG
hsa-mir- hsa-mir- hsa-miR- MIMAT0000689 CACCCGUAGAACCGACCUUGCG 757
99b 99b 99b 366 AACAUAGAGGAAAUUCCACGU hsa-mir- hsa-mir- hsa-mir-
AACAUAGAGGAAAUUCCACGU 758 376c 376c 376c 367 AUCAUAGAGGAAAAUCCAUGUU
hsa-mir- hsa-mir- hsa-mir- AUCAUAGAGGAAAAUCCAUGUU 759 376b 376b
376b 368 UGGUGGGCCGCAGAACAUGUGC hsa-mir- hsa-mir- hsa-mir-
UGGUGGGCCGCAGAACAUGUGC 760 654 654 654 369 AUCAUAGAGGAAAAUCCACGU
hsa-mir- hsa-mir- hsa-mir- AUCAUAGAGGAAAAUCCACGU 761 376a-2 376a-2
376a-2 370 AUCAUAGAGGAAAAUCCACGU hsa-mir- hsa-mir- hsa-mir-
AUCAUAGAGGAAAAUCCACGU 762 376a-1 376a-1 376a-1 371
AAAACCGUCUAGUUACAGUUGU hsa-mir- 1537 372 AUAUACAGGGGGAGACUCUCAU
hsa-mir- 1185-2 373 CCUAGAAUGGGGAUUGUGGG hsa-mir- 1301* 374
AGCGAGACCUCAACUCUACAAU hsa-mir- 1303 375 UCGACCGGACCUCGACCGGCUC
hsa-mir- 1307* 376 AUGUAGGGAUGGAAGCCAUGAA hsa-mir-
135a-2 377 CUCACUGAACAAUGAAUGCAA hsa-mir- 181b-1* 378
CGGGUAGAGAGGGCAGUGGGAG hsa-mir- 197* 379 GCUGGGAAGGCAAAGGGACGU
hsa-mir- 204* 380 ACCUUGGCUCUAGACUGCUUAC hsa-mir- 212* 381
GCUCUGACUUUAUUGCACUACU hsa-mir- 301a 382 AGGGACUUUUGGGGGCAGAUGU
hsa-mir- 365-1 383 UUGGGGACAUUUUGCAUUCAU hsa-mir- 450a-2* 384
AAUGUGUAGCAAAAGACAGA hsa-mir- 511-1-3p 385 AAUGUGUAGCAAAAGACAGA
hsa-mir- 511-2-3p 386 AUCAUACAAGGACAAUUUCUUU hsa-mir- 539 387
AAAGGUAAUUGCAGUUUUUCCC hsa-mir- 570 388 UCGCGGUUUGUGCCAGAUGACG
hsa-mir- 579 389 AGAAGGCACUAUGAGAUUUAGA hsa-mir- 605 390
AGACACAUUUGGAGAGGGAACC hsa-mir- 642 391 AGGACCUUCCCUGAACCAAGGA
hsa-mir- 659 392 AUAUACAGGGGGAGACUCUUAU hsa-mir- 1185-1
TABLE-US-00017 TABLE 32 Novel miRNAs identified by deep sequencing
analysis. SEQ Temporary SEQ ID Mature Assigned miRBase ID NO.
Sequence Captured miRNA ID ID NO. miRNA Precursor Sequence 763
CCAAAACUGCAGUUACUUUUG has-mir-548o- pending 1057
UggUgcaaaagUaaUUgcggUUUUUgccaUUaaaagUaaUgc 2
ggCCAAAACUGCAGUUACUUUUGcaccc 764 GAGCCUGGAAGCUGGAGCCUGC
hsa-mir-1254- hsa-mir- 1058 cUGAGCCUGGAAGCUGGAGCCUGCagUgagcUaUgaUca
2 1254-2 UgUcccUgUacUcUagccUgggca 765 CGGGCGUGGUGGUGGGGGUG
hsa-mir-1268b hsa-mir- 1059 accCGGGCGUGGUGGUGGGGGUGggUgccUgUaaUUcca
1268b gcUagUUggga 766 UCGAGGAGCUCACAGUCUAGA hsa-miR-151- hsa-mir-
1060 agUcUcUcUUcagggcUcccgagacacagaaacagacaccUgccc 5p-2 151b
UCGAGGAGCUCACAGUCUAGAca 767 UAAGGUGCAUCUAGUGCAGUU hsa-mir-18b-
hsa-mir- 1061 UcUUgUgUUAAGGUGCAUCUAGUGCAGUUagUgaagcag 2 18b
cUUagaaUcUacUgcccUaaaUgccccUUcUggcacaggc 768 GAGGGUUGGGUGGAGGC
hsa-miR-296- hsa-miR- 1062
cUgccUccaccccgccUggccUgacccagccagggcUcUagGAGG 2 296-2
GUUGGGUGGAGGCaa 769 AUCAUAGAGGAAAAUCCAUGUU hsa-mir-376b- hsa-mir-
1063 UaaaacgUggaUaUUccUUcUaUgUUUacgUgaUUccUggU 2 376b
UaAUCAUAGAGGAAAAUCCAUGUUUUc 770 AACAUAGAGGAAAUUCCACGU hsa-mir-376c-
hsa-mir- 1064 UaaaaggUggaUaUUccUUcUaUgUUUaUgUUaUUUaUgg 2 376c
UUaAACAUAGAGGAAAUUCCACGUUUUc 771 ACUGGACUUGGAGUCAGAAA hsa-mir-378b-
hsa-mir- 1065 acUgUUUcUgUccUUgUUcUUgUUgUUaUUACUGGACU 1 378d-1
UGGAGUCAGAAAcagg 772 ACUGGACUUGGAGUCAGAAA hsa-mir-378b- hsa-mir-
1066 aggagagaacACUGGACUUGGAGUCAGAAAacUUUcaUcc 2 378d-2
aagUcaUUcccUgcUcUaagUcccaUUUcUgUUcca 773 ACUGGACUUGGAGUCAGGA
hsa-miR-378c hsa-mir- 1067
cUgacUccagUgUccaggccaggggcagacagUggacagagaacag 378e
UgcccaagaccACUGGACUUGGAGUCAGGAcaU 774 AGGCAGUGUAUUGCUAGCGGCU
hsa-mir-449c hsa-mir- 1068 UcaggUAGGCAGUGUAUUGCUAGCGGCUgUUaaUgaUU
449c UUaacagUUgcUagUUgcacUccUcUcUgU 775 UGCACCCAGGCAAGGAUUCUGC
hsa-mir-500- hsa-mir- 1069
gUUcccccUcUcUaaUccUUgcUaccUgggUgagagUgcUUUc 2 500b
UgaaUgcagUGCACCCAGGCAAGGAUUCUGCaagggggag U 776
UAAUCCUUGCUACCUGGGUGAG hsa-mir-500- has-mir- 1070
gUUcccccUcUcUAAUCCUUGCUACCUGGGUGAGagUgc 2* 500b
UUUcUgaaUgcagUgcacccaggcaaggaUUcUgcaagggggag U 777
AAAAGUAAUCGCGGUUUUUGUC hsa-mir-548h- hsa-mir- 1071
acAAAAGUAAUCGCGGUUUUUGUCaUUacUUUUaacUg 5 548h-5
UaaaaaccacggUUgcUUUUgc 778 AAAAGUAAUUGUGGAUUUUGCU hsa-mir-548r-
hsa-mir- 1072 aUgUUggUgcAAAAGUAAUUGUGGAUUUUGCUaUUacU 1 548ab
UgUaUUUaUUUgUaaUgcaaaacccgcaaUUagUUUUgcacc aacc 779
AAAAGUAAUUGUGGAUUUUGCU hsa-mir-548r- has-mir- 1073
UgUUggUgcAAAAGUAAUUGUGGAUUUUGCUaUUacU 2 548ab
UgUaUUUaUUUgUaaUgcaaaacccgcaaUUagUUUUgcacc aacc 780
UGUCUUACUCCCUCAGGCACAU hsa-miR-550- pending 1074
cUggUgcagUgccUgagggagUaagagUccUgUUgUUgUaaga 3*
UagUGUCUUACUCCCUCAGGCACAUcUccaa 781 GGUGGGCUUCCCGGAGGG hsa-mir-2221
hsa-mir- 1075 gaaaacaaccaGGUGGGCUUCCCGGAGGGcggaacacccagc 4417
cccagcaUccagggcUcaccUaccacgUUUg 782 GAAGCGGUGGCUGGGCUG hsa-mir-2222
pending 1076 gaggcggcccUagcgccaUUUUgUgggagcGAAGCGGUGGCU
GGGCUGcgcUUg 783 CACUGCAGGACUCAGCAG hsa-mir-2223 hsa-mir- 1077
UggUUUUUgcUcUgagUgaccgUggUggUUgUgggagUCAC 4418
UGCAGGACUCAGCAGgaaUUc 784 UGAGGGAGGAGACUGCA hsa-mir-2224 hsa-mir-
1078 UggUggUgUgUgccUgUagUcUUagcUacUcgggaggcUGAG 4419a
GGAGGAGACUGCAgUgagUggaggUcacgccacUg 785 ACUGGACUUGGAGCCAGAAG
hsa-mir-2225 hsa-mir- 1079
gUcaggUccUggacUcccaUagUUUUcaggcUgcUaaacaacag 378f
aacgagcACUGGACUUGGAGCCAGAAGUcUUggg 786 GUCACUGAUGUCUGUAGCUGAG
hsa-mir-2226 hsa-mir- 1080
cUcUUggUaUgaacaUcUgUgUgUUcaUgUcUcUcUgUgca 4420
caggggacgagaGUCACUGAUGUCUGUAGCUGAGac 787 GAUGAGGAUGGAUAGCAAGGAA
hsa-mir-2227 hsa-mir- 1081
cUggccUcUgUgccUggaUacUUUaUacgUgUaaUUgUGAU 3605
GAGGAUGGAUAGCAAGGAAgccgc 788 ACCUGUCUGUGGAAAGGAGCUA hsa-mir-2228
hsa-mir- 1082 cUgggUcUccUUUcUgcUgagagUUgaacacUUgUUgggaca 4421
ACCUGUCUGUGGAAAGGAGCUAccUac 789 AAAAGCAUCAGGAAGUACCCA hsa-mir-2229
hsa-mir- 1083 agUUcUUcUgcagacAAAAGCAUCAGGAAGUACCCAccaU 4422
gUaccagUgggcccUUcUUgaUgcUcUUgaUUgcagaggagcc 790
UGCCUGGAACAUAGUAGGGACU hsa-mir-2230- hsa-mir- 1084
UccUUUaUUgagUcccUacUaUgUUccaggcaccUacgaUacc 1 3116-2
cagUGCCUGGAACAUAGUAGGGACUcaaUaaagU 791 UGCCUGGAACAUAGUAGGGACU
hsa-mir-2230- hsa-mir- 1085
ccUUUaUUgagUcccUacUaUgUUccaggcaccUacgaUaccc 2 3116-1
agUGCCUGGAACAUAGUAGGGACUcaaUaaagU 792 UGGAUUAAAAACAAUGGAGG
hsa-mir-2231 pending 1086
cgccUccaUgUUUcagcaUcUaUgUcaUgggcUUggUccUgga gUGGAUUAAAAACAAUGGAGGU
793 AUAGGCACCAAAAAGCAACAA hsa-mir-2232 hsa-mir- 1087
aUcaUgUacUgcagUUgccUUUUUgUUcccaUgcUgUUUaa 4423
gccUagcAUAGGCACCAAAAAGCAACAAcagUaUgUgaa 794 ACUGGGCUUGGAGUCAGAAG
hsa-mir-2233 hsa-mir- 1088
cACUGGGCUUGGAGUCAGAAGaccUggcUccagcccagcUc 378g
gUaUUaggUUggUgcaaaagUUaUUgUggUUUUUgcUaUU 795 CAAAAACCGGCAAUUACUUUUG
hsa-mir-2234 hsa-mir- 1089 UUUUUUUaaUggCAAAAACCGGCAAUUACUUUUGcacU
548ac aaccUagUag 796 GUCAAAUGAAGGGCUGAUCACG hsa-mir-2235 pending
1090 aaagUGUCAAAUGAAGGGCUGAUCACGaaaUagcgcaUU agcUcUUUUUUUgaaaacUUg
797 AAAGGGAGGAUUUGCUUAGAAGG hsa-mir-2236 pending 1091
gacUggcUacgUagUUcgggcaaaUccUccaaaagggAAAGGG AUGG
AGGAUUUGCUUAGAAGGAUGGcgcUcc 798 AGAGUUAACUCAAAAUGGACUA hsa-mir-2237
hsa-mir- 1092 cUUacaUcacacacAGAGUUAACUCAAAAUGGACUAaUU 4424
UUUccacUagUUagUccaUUUcaagUUaacUcUgUgUgUga UgUagU 799
UGUUGGGAUUCAGCAGGACCAU hsa-mir-2238 hsa-mir- 1093
gUgcUUUacaUgaaUggUcccaUUgaaUcccaacagcUUUgcg 4425
aagUgUUGUUGGGAUUCAGCAGGACCAUUcgUgUaaag Uaa 800
UAAAUAGAGUAGGCAAAGGACA hsa-mir-2239 hsa-mir- 1094
UaaaUggUUaUgUccUUUgccUaUUcUaUUUaagacacccU 3121
gUaccUUAAAUAGAGUAGGCAAAGGACAgaaacaUUUU 801 AGAGUCGAGAGUGGGAGAAGAG
hsa-mir-2240 pending 1095 gcAGAGUCGAGAGUGGGAGAAGAGcggagcgUgUgagcag
UacUgcggccUccUcUccUcUccUaaccUcgcUcUc 802 GAAGAUGGACGUACUUU
hsa-mir-2241 hsa-mir- 1096 agUUGAAGAUGGACGUACUUUgUcUgacUacaaUaUUca
4426 aaaggagUcUacUcUUcaUcUUg 803 UCUGAAUAGAGUCUGAAGAGU hsa-mir-2242
hsa-mir- 1097 gaagccUcUUggggcUUaUUUagacaaUggUUUcaUcaUUUc 4427
gUCUGAAUAGAGUCUGAAGAGUcUUU 804 CAAGGAGACGGGAACAUGGAGC hsa-mir-2243
hsa-mir- 1098 UUggcaggUgccaUgUUgccUgcUccUUacUgUacacgUggcU 4428
ggCAAGGAGACGGGAACAUGGAGCcgccaU 805 AAGAGGAAGAAAUGGCUGGUUC
hsa-mir-2244 hsa-mir- 1099 aggaagAAGAGGAAGAAAUGGCUGGUUCUcaggUgaaUg
3916 UgUcUgggUUcaggggaUgUgUcUccUcUUUUcU 806 UUCGCGGGCGAAGGCAAAGUC
hsa-mir-2245 hsa-mir- 1100 ggcgggcUUCGCGGGCGAAGGCAAAGUCgaUUUccaaaag
3124 UgacUUUccUcacUcccgUgaagUcggcg 807 AAAAGCUGGGCUGAGAGGCG
hsa-mir-2246 hsa-mir- 1101 agggagAAAAGCUGGGCUGAGAGGCGacUggUgUcUaaU
4429 UUgUUUgUcUcUccaacUcagacUgccUggccca 808 AUGGCCAAAACUGCAGUUAUUU
hsa-mir-2247 hsa-mir- 1102 cUgcaaaaaUaaUUgcagUUUUUgccaUUaUUUUUaaUaa
548s UUaUaaUaAUGGCCAAAACUGCAGUUAUUUUUgcac 809
UAGUGGAUGAUGCACUCUGUGC hsa-mir-2248 hsa-mir- 1103
cUacUUccagUAGUGGAUGAUGCACUCUGUGCagggccaa 3681
cUgUgcacacagUgcUUcaUccacUacUggaagUgU 810 AGGCUGGAGUGAGCGGAG
hsa-mir-2249 hsa-mir- 1104 gUgAGGCUGGAGUGAGCGGAGaUcgUaccacUgcacUcca
4430 accUggUga 811 GAAAACGACAAUGACUUUUGCA hsa-mir-2250 hsa-mir-
1105 cUgUUaggUUggUgcaaaagUaaUUgUggUUUUUgaaagUa 548ad
acUUggcGAAAACGACAAUGACUUUUGCAccaaUcUaaUa c 812
GCGACUCUGAAAACUAGAAGGU hsa-mir-2251 hsa-mir- 1106
UggUUUGCGACUCUGAAAACUAGAAGGUUUaUgacUgg 4431
gcaUUUcUcacccaaUgcccaaUaUUgaacUUUcUagUUgUc agagUcaUUaaccc 813
AGAAGGCUGGAGCGCGGCGGU hsa-mir-2252 pending 1107
gcacUgcggUUcUgaggccgUUacUccggcUUcUccaUagaggg
cggAGAAGGCUGGAGCGCGGCGGUga 814 AAAGACUCUGCAAGAUGCCU hsa-mir-2253
hsa-mir- 1108 gcaUcUUgcagagccgUUccaaUgcgacaccUcUagagUgUcaU 4432
ccccUagaaUgUcaccUUggAAAGACUCUGCAAGAUGCCU 815 ACAGGAGUGGGGGUGGGACAU
hsa-mir-2254 hsa-mir- 1109
caUccUccUUacgUcccaccccccacUccUgUUUcUggUgaaaU 4433
aUUcaaACAGGAGUGGGGGUGGGACAUaaggaggaUa 816 AGGAGAAGUAAAGUAGAA
hsa-mir-2255 hsa-mir- 1110 UcacUUUAGGAGAAGUAAAGUAGAAcUUUggUUUUcaa
4434 cUUUUccUacagUgU 817 AUGGCCAGAGCUCACACAGAGG hsa-mir-2256
hsa-mir- 1111 aggcagcaaAUGGCCAGAGCUCACACAGAGGgaUgagUgca 4435-1
cUUcaccUgcagUgUgacUcagcaggccaacagaUgcUa 818 GCAGGACAGGCAGAAGUGGAU
hsa-mir-2257 hsa-mir- 1112
gccUcacUUUUccacUUaUgccUgcccUgccccUcgaaUcUgcU 4436
ccacgaUUUggGCAGGACAGGCAGAAGUGGAUaagUgagg a 819
AUCAGGGCUUGUGGAAUGGGAA hsa-mir-2258 hsa-mir- 1113
ggcccAUCAGGGCUUGUGGAAUGGGAAggagaagggacgc 3127
UUccccUUcUgcaggccUgcUgggUg 820 AUGGCCAGAGCUCACACAGAGG hsa-mir-2259
hsa-mir- 1114 gcaaAUGGCCAGAGCUCACACAGAGGgaUgagUgcacUUc 4435-2
accUgcagUgUgacUcagcaggccaacagaUgcU 821 UGAGGAUAUGGCAGGGAAGGGG
hsa-mir-2260 hsa-mir- 1115
acgUggUGAGGAUAUGGCAGGGAAGGGGagUUUcccUc
3679 UaUUcccUUccccccagUaaUcUUcaUcaUgc 822 AGGAGGUUGGUGUGGAUU
hsa-mir-2261 pending 1116 UaUgAGGAGGUUGGUGUGGAUUcUgUUgaagaaaaagaa
ggggaacacUaaUUUUccaUU 823 UUGGAAGACAUGGAGCAUGAGG hsa-mir-2262
pending 1117 UUUUGGAAGACAUGGAGCAUGAGGUaagUgccUagaUcc
UcaaaccacUUgccUccaccUaUgcUUccaggU 824 UCUGGCAAGUAAAAAACUCUCA
hsa-mir-2263 hsa-mir- 1118 cUUccUCUGGCAAGUAAAAAACUCUCAUUUUccUUaaaa
3128 aaUgagagUUUUUUacUUgcaaUaggaaa 825 UGGGCUCAGGGUACAAAGGUU
hsa-mir-2264 hsa-mir- 1119
acUUUgUgcaUUgggUccacaaggaggggaUgacccUUgUGG 4437 GCUCAGGGUACAAAGGUU
826 CAAAAACUGCAAUUACUUUCA hsa-mir-2265- hsa-mir- 1120
gcagUUUUUgccaUUaagUUgcggUUUUUgccaUUaUaaUg 1 548ae-1
gCAAAAACUGCAAUUACUUUCAcaccUgc 827 CAAAAACUGCAAUUACUUUCA
hsa-mir-2265- hsa-mir- 1121
UgUgcaaaagUaaUUgUggUUUUUgUcaUUUaaaagUaaUg 2 548ae-2
gCAAAAACUGCAAUUACUUUCAcacc 828 GCUGCACCGGAGACUGGGUAA hsa-mir-2266-
hsa-mir- 1122 acUUgUcaUgUcUUacccagUcUccggUgcagccUgUUgUcaa 1 3130-1
gGCUGCACCGGAGACUGGGUAAgacaUgacaagc 829 GCUGCACCGGAGACUGGGUAA
hsa-mir-2266- hsa-mir- 1123
UgUcUUacccagUcUccggUgcagccUUgacaacagGCUGCAC 2 3130-2
CGGAGACUGGGUAAgacaUgacaagUU 830 AUCCCCAGAUACAAUGGACAAU hsa-mir-2267
hsa-mir- 1124 cgUgUcAUCCCCAGAUACAAUGGACAAUaUgcUaUUaUa 2355
aUcgUaUggcaUUgUccUUgcUgUUUggagaUaaUacU 831 AUUGUCCUUGCUGUUUGGAGAU
hsa-mir-2267* pending 1125
cgUgUcaUccccagaUacaaUggacaaUaUgcUaUUaUaaUcg
UaUggcAUUGUCCUUGCUGUUUGGAGAUaaUacU 832 CACAGGCUUAGAAAAGACAGU
hsa-mir-2268 hsa-mir- 1126
UaagUgUaaacUUaaggacUgUcUUUUcUaagccUgUgccUU 4438
gccUUUccUUUggCACAGGCUUAGAAAAGACAGUcUUUa agUUUacacUUc 833
UCGAGGACUGGUGGAAGGGCCU hsa-mir-2269 hsa-mir- 1127
UcUcagagUCGAGGACUGGUGGAAGGGCCUUUccccUca 3131
gaccaaggcccUggccccagcUUcUUcUcagagU 834 GUGACUGAUACCUUGGAGGCAU
hsa-mir-2270 hsa-mir- 1128 ccaGUGACUGAUACCUUGGAGGCAUUUUaUcUaagaUac
4439 acacaaagcaaaUgccUcUaaggUaUcagUUUaccaggcca 835
AGGGCUGGACUCAGCGGCGGAG hsa-mir-2271 pending 1129
gcgcagAGGGCUGGACUCAGCGGCGGAGcUggcUgcUggc
cUcagUUcUgccUcUgUccaggUccUUgUga 836 UGUCGUGGGGCUUGCUGGCUUG
hsa-mir-2272 hsa-mir- 1130
cUcUcaccaagcaagUgcagUggggcUUgcUggcUUgcaccgUg 4440
acUcccUcUcaccaagcaagUGUCGUGGGGCUUGCUGGCU UGcacUgUgaagaU 837
ACAGGGAGGAGAUUGUA hsa-mir-2273 hsa-mir- 1131
cagagUcUccUUcgUgUacagggaggagacUgUacgUgagagaU 4441
agUcagaUccgcaUgUUagagcagagUcUccUUcgUgUACAG GGAGGAGAUUGUAc 838
ACUGGACUUGGAGGCAGAA hsa-mir-2274 hsa-mir- 1132
UggUcaUUgagUcUUcaaggcUagUggaaagagcACUGGACU 378b UGGAGGCAGAAagaccc
839 GCCGGACAAGAGGGAGG hsa-mir-2275 hsa-mir- 1133
gcgcccUcccUcUcUccccggUgUgcaaaUgUgUgUgUgcggUg 4442
UUaUGCCGGACAAGAGGGAGGUg 840 AUACACAUACACGCAACACACA hsa-mir-2276
hsa-mir- 1134 aUgUgUgUgUaUaUgUgUgUUgcaUgUgUgUaUaUgUgUg 466
UaUaUaUgUacacAUACACAUACACGCAACACACAUaUa UacaUgcac 841
UUGGAGGCGUGGGUUUU hsa-mir-2277 hsa-mir- 1135
ggUgggggUUGGAGGCGUGGGUUUUagaaccUaUcccUU 4443 UcUagcccUgagca 842
AAUUCCCUUGUAGAUAACCCGG hsa-mir-2278 hsa-mir- 1136
cgaUcacUagaUUaUcUacaagggaaUUUUUUUUUaaUUU 3938
aaaaAAUUCCCUUGUAGAUAACCCGGUggUca 843 AAGACUGGAGACAAAGUGGGAG
hsa-mir-2279 pending 1137 CACCACCAAAAUCUCCAGGGGCAUCGUUGAAAUCGUA
AGGGAUGUGCAGCUCAUUAAGACUGGAGACAAAGUG GGAG 844
CUGACUGAAUAGGUAGGGUCAU hsa-mir-2280 hsa-mir- 1138
aaaCUGACUGAAUAGGUAGGGUCAUUUUUcUgUgacUg 3136
cacaUggcccaaccUaUUcagUUagUUc 845 CUCGAGUUGGAAGAGGCG hsa-mir-2281
hsa-mir- 1139 gUgacgacUggccccgccUcUUccUcUcggUcccaUaUUgaaCU 4444
CGAGUUGGAAGAGGCGagUccggUcUcaaa 846 AGAUUGUUUCUUUUGCCGUGCA
hsa-mir-2282 hsa-mir- 1140 UUccUgcAGAUUGUUUCUUUUGCCGUGCAagUUUaagU
4445 UUUUgcacggcaaaagaaacaaUccagagggU 847 CACGGCAAAAGAAACAAUCCA
hsa-mir-2282* has-mir- 1141
UUccUgcagaUUgUUUcUUUUgccgUgcaagUUUaagUUU 4445
UUgCACGGCAAAAGAAACAAUCCAgagggU 848 CAGGGCUGGCAGUGACAUGGGU
hsa-mir-2283 hsa-mir- 1142
cUggUccaUUUcccUgccaUUcccUUggcUUcaaUUUacUcc 4446
CAGGGCUGGCAGUGACAUGGGUcaa 849 GGUGGGGGCUGUUGUUU hsa-mir-2284
hsa-mir- 1143 gUUcUagagcaUggUUUcUcaUcaUUUgcacUacUgaUacU 4447
UggggUcagaUaaUUgUUUgUGGUGGGGGCUGUUGUUU gcaUUgUaggaU 850
UGGGGAGGUGUGGAGUCAGCAU hsa-mir-2285 pending 1144
gggcaUGGGGAGGUGUGGAGUCAGCAUggggcUaggaggc
cccgcgcUgacccgccUUcUccgcagcUg
accUgagcaccaUUUacUgagUccUUUgUUcUcUacUagUUU 851
GCAGAGAACAAAGGACUCAGU hsa-mir-2286 hsa-mir- 1145
gUagUagUUcgUaGCAGAGAACAAAGGACUCAGUaaaUg 3919 gUgcUcagga 852
GGCUCCUUGGUCUAGGGGUA hsa-mir-2287 hsa-mir- 1146
aggagUgaccaaaagacaagagUgcgagccUUcUaUUaUgcccag 4448
acagggccaccagagGGCUCCUUGGUCUAGGGGUAaUgcca 853
CGUCCCGGGGCUGCGCGAGGCA hsa-mir-2288 hsa-mir- 1147
agcagcccUcggcggcccggggggcgggcggcggUgccCGUCCCG 4449
GGGCUGCGCGAGGCAcaggcg 854 AAAGGUAAUUGUGGUUUCUGC hsa-mir-2289-
hsa-mir- 1148 gUgcAAAGGUAAUUGUGGUUUCUGCUUUUaaaggUaaU 1 548ag-1
ggcaaaUaUUacaUUUacUUUUgcacca 855 AAAGGUAAUUGUGGUUUCUGC
hsa-mir-2289- hsa-mir- 1149 UgcAAAGGUAAUUGUGGUUUCUGCcaUUgaaagUaaagg
2 548ag-2 caagaaccUcaaUUaccUUUgcagc 856 UGGGGAUUUGGAGAAGUGGUGA
hsa-mir-2290 hsa-mir- 1150 UgUcUGGGGAUUUGGAGAAGUGGUGAgcgcaggUcUUU
4450 ggcaccaUcUccccUggUcccUUggcU 857 AAAAGUGAUUGCAGUGUUUG
hsa-mir-2291 hsa-mir- 1151 aggUUggUgcAAAAGUGAUUGCAGUGUUUGccaaUaaaa
548ah gUaaUgacaaaaacUgcagUUacUUUUgcaccagccc 858 UGGUAGAGCUGAGGACA
hsa-mir-2292 hsa-mir- 1152
UcUgUaccUcagcUUUgcUcccaaccaaccacUUccacaUgUU 4451
UUgcUGGUAGAGCUGAGGACAgc 859 UUGAAUUCUUGGCCUUAAGUGAU hsa-mir-2293
hsa-mir- 1153 UggaUcacUUgaggccaagagUgcaaggcUgUagUgUgcacagc 4452
cUUGAAUUCUUGGCCUUAAGUGAUccc 860 UAGGAGCUCAACAGAUGCCUGU hsa-mir-2294
hsa-mir- 1154 UcagagUAGGAGCUCAACAGAUGCCUGUUgacUgaaUaa 3139
UaaacaggUaUcgcaggagcUUUUgUUaUgU 861 AGCUUUUGGGAAUUCAGGUAG
hsa-mir-2295 hsa-mir- 1155
UgUccUcUUgaggUaccUgaaUUaccaaaagcUUUaUgUaUU 3140
cUgaagUUaUUgaaaaUaagAGCUUUUGGGAAUUCAGGU AGUUcaggagUgacU 862
GAGCUUGGUCUGUAGCGGUU hsa-mir-2296 hsa-mir- 1156
UggaGAGCUUGGUCUGUAGCGGUUUccUUggggcaggUg 4453
gggacUgcUccUUUgggaggaaggaggaggcccaggccgcgUcUU cagg 863
GGAUCCGAGUCACGGCACCA hsa-mir-2297 hsa-mir- 1157
ccGGAUCCGAGUCACGGCACCAaaUUUcaUgcgUgUccgU 4454 gUgaagagaccacca 864
CAAAAGUGAUCGUGGUUUUUG hsa-mir-2298 hsa-mir- 1158
gUggUgCAAAAGUGAUCGUGGUUUUUGcaaUUUUUUaa 548t
UgacaaaaaccacaaUUacUUUUgcaccaa 865 AGGGUGUGUGUGUUUUU hsa-mir-2299
hsa-mir- 1159 agaAGGGUGUGUGUGUUUUUccUgagaaUaagagaaggaa 4455
ggacagccaaaUUcUUca 866 CCUGGUGGCUUCCUUUU hsa-mir-2300 hsa-mir- 1160
aUgaaCCUGGUGGCUUCCUUUUcUgggaggaagUUagggU 4456 Uca 867
UCACAAGGUAUUGACUGGCGUA hsa-mir-2301 hsa-mir- 1161
ggagUacUccagUcaaUaccgUgUgagUUagaaaagcUcaaUU 4457
CACAAGGUAUUGACUGGCGUAUUca 868 AGAGGUAGGUGUGGAAGAA hsa-mir-2302
hsa-mir- 1162 gagcgcacAGAGGUAGGUGUGGAAGAAagUgaaacacUaU 4458
UUUaggUUUUagUUacacUcUgcUgUggUgUgcUg 869 CCAGGAGGCGGAGGAGGUGGAG
hsa-mir-2303 hsa-mir- 1163 acCCAGGAGGCGGAGGAGGUGGAGgUUgcagUgagccaag
4459 aUcgUggcacUgacUccagccUgggg 870 GUGGAGGACUGAGAAGGUGAG
hsa-mir-2304 pending 1164 aUgaUGUGGAGGACUGAGAAGGUGAGgcagUUUUgcccc
gUgcUgccUUccaccggUUaagaccUccaaaaUcga 871 ACUGACAGGAGAGCAUUUUGA
hsa-mir-2305 hsa-mir- 1165
ggacaaaaUUaaaaUgcUcUUcUgUcaUUgUaaUagUUcaUa 3660
UgggcACUGACAGGAGAGCAUUUUGAcUUUgUca 872 AGCGCGGGCUGAGCGCUGCCAG
hsa-mir-2306 hsa-mir- 1166
ggUUcacUggUcgUgcUUccUgcgggcUgAGCGCGGGCUGA 2277 GCGCUGCCAGUcagcg 873
AAGCUCGGGCGCUCCGGCUGU hsa-mir-2307 pending 1167
gcUcagUcagcUgggccgccUcagcUcUcggagUaggAAGCUCG GGCGCUCCGGCUGUaaggagcc
874 AUAGUGGUUGUGAAUUUACCUU hsa-mir-2308 hsa-mir- 1168
gUUUUUUgcccAUAGUGGUUGUGAAUUUACCUUcUccU 4460
cUUUgcagUgaUaaaggaggUaaaUUcacaaccacUgUgggcag aaac 875
GAUUGAGACUAGUAGGGCUAGGC hsa-mir-2309 hsa-mir- 1169
gagUaggcUUaggUUaUgUacgUagUcUaggccaUacgUgUU 4461
ggaGAUUGAGACUAGUAGGGCUAGGCcUacUg 876 CUACCCCAGGAUGCCAGCAUAG
hsa-mir-2310 pending 1171
aUagcUggUUggcaUUcUggcccUggUUcaUgccaacUcUUg
UgUUgaCUACCCCAGGAUGCCAGCAUAGUUgc 877 ACUGGACUUGGUGUCAGAUGG
hsa-mir-2311 hsa-mir- 1171 acaggaacACUGGACUUGGUGUCAGAUGGgaUgagcccUg
378h gcUcUgUUUccUagcagcaaUcUgaUcUUgagcUagUcacUgg 878
GACAAUGAUGAGAAGACCUGAG hsa-mir-2312 pending 1172
gggGACAAUGAUGAGAAGACCUGAGgaUUUgcagccccca
gcccUgggUUcaagUcccagcUcUaccccUUcUUggcccc 879 ACCGCUCGAUCUUGGGACC
hsa-mir-2313 pending 1173 gUUUcACCGCUCGAUCUUGGGACCcaccgcUgcccUcagcU
ccgagUccagggcgaggUaagggcUggagUcgggcagga 880 UAGUGGAUGAUGGAGACUCGGU
hsa-mir-2314 hsa-mir- 1174 aUUgaggcacUgggUAGUGGAUGAUGGAGACUCGGUacc
3691 cacUgcUgagggUggggaccaagUcUgcgUcaUccUcUccUcag UgccUcaaa 881
CUCGGGAGCGUUAGAGAUGGA hsa-mir-2315 pending 1175
gUCUCGGGAGCGUUAGAGAUGGAgacUaacgUcUUccaa
gggagaUUgcgUcUccacUUUcacccUggUacUgagag 882 GGCUGGAGCGAGUGCAGUGGUG
hsa-mir-2316 hsa-mir- 1176 UgcccaGGCUGGAGCGAGUGCAGUGGUGcagUcagUccU
3135b agcUcacUgcagccUcgaacUccUgggcU 883 UCAGGUGUGGAAACUGAGGCAG
hsa-mir-2317 hsa-mir- 1177 UUUUCAGGUGUGGAAACUGAGGCAGgaggcagUgaagUa
3934 acUUgcUcaggUUgcacagcUgggaagU 884 UGACACGGAGGGUGGCUUGGGAA
hsa-mir-2318 hsa-mir- 1178
cUUcccagcUgcccUaagUcaggagUggcUUUccUGACACGG 4462 AGGGUGGCUUGGGAAa
885 GAAGAUGGUGCUGUGCUGAGGA hsa-mir-2319 pending 1179
ggUGAAGAUGGUGCUGUGCUGAGGAaaggggaUgcagagc ccUgcccagcaccaccaccUccUaUg
886 AAAGACUGCAAUUACUUUUGCG hsa-mir-2320 hsa-mir- 1180
aUggUgcaaaagUaaUgUggUUUUUUUcUUUacUUUUaaU 548u
ggcAAAGACUGCAAUUACUUUUGCGcca 887 GAGACUGGGGUGGGGCC hsa-mir-2321
hsa-mir- 1181 aaUagaUUaUUggUcaccaccUccagUUUcUgaaUUUgUGA 4463
GACUGGGGUGGGGCCUgagaaUUUgc 888 AAGGUUUGGAUAGAUGCAAUA hsa-mir-2322
hsa-mir- 1182 ggaaccUUagUAAGGUUUGGAUAGAUGCAAUAaagUaUg 4464
UccacagcUgaaaggacaUacUUUaUUgcaUgUaUccaaaccU UacUaaUUca 889
AAAGGUAAUUGCAGUUUUUCCC hsa-mir-2323 hsa-mir- 1183
gUaUUaggUUggUgcAAAGGUAAUUGCAGUUUUUCCCa 548ai
UUUaaaaUaUggaaaaaaaaaUcacaaUUacUUUUgcaUcaa ccUaaUaa 890
AGGGGACCAAAGAGAUAUAUAG hsa-mir-2324 hsa-mir- 1184
gaaacUacacUUUaAGGGGACCAAAGAGAUAUAUAGaUa 3144
UcagcUaccUaUaUaccUgUUcggUcUcUUUaaagUgUagU UUa 891
UAAAAACUGCAAUUACUUUUA hsa-mir-2325- hsa-mir- 1185
aUUggUgUaaaagUaaUUgcaggUUaUgccaUUaaaagUaaU 1 548aj-1
ggUAAAAACUGCAAUUACUUUUAcacUaac 892 UAAAAACUGCAAUUACUUUUA
hsa-mir-2325- hsa-mir- 1186
aaggUaUUaggUUggUgcaaaagUaaUUgcagUUUUUgcUa 2 548aj-2
UUacUUUUaaUggUAAAAACUGCAAUUACUUUUAcacca accUaaUaUUUa 893
CUCAAGUAGUCUGACCAGGGGA hsa-mir-2326 hsa-mir- 1187
caUgUgUccccUggcacgcUaUUUgaggUUUacUaUggaacC 4465
UCAAGUAGUCUGACCAGGGGAcacaUga 894 UCUGGCUGAGGAGGAAGUGGAG
hsa-mir-2327- pending 1188
gcUcUagUagccacagccaUccccUagagggaUCUGGCUGAGG 1 AGGAAGUGGAGg 895
UCUGGCUGAGGAGGAAGUGGAG hsa-mir-2327- pending 1189
ccgccUcagUggcUUccUccacagccaccUccggagggaUCUGGC 2
UGAGGAGGAAGUGGAGgUgUcacUgg 896 GGCGACAAAACGAGACCCUGU hsa-mir-2328
hsa-mir- 1190 cUgGGCGACAAAACGAGACCCUGUcUUUUUUUUUUUc 1273c
UgagacagagUcUcgUUcUgUUgcccaa 897 GGGUGCGGGCCGGCGGGG hsa-mir-2329
hsa-mir- 1191 acgcGGGUGCGGGCCGGCGGGGUagaagccacccggcccggc 4466
ccggcccggcga 898 CCUGCUGGUCAGGAGUGGAUAC hsa-mir-2330 hsa-mir- 1192
gccaUUCCUGCUGGUCAGGAGUGGAUACUggagcaaUag 3692
aUacagUUccacacUgacacUgcagaagUgga 899 CAUGCUAGGAUAGAAAGAAUGG
hsa-mir-2331 hsa-mir- 1193 aUUUUcUUUgcUaagUcccUUcUUUcUaUccUagUaUaac
3146 UUgaagaaUUcaaaUagUCAUGCUAGGAUAGAAAGAAUG GgacUUggccagggaagaa
900 AAUGUGGAAGUGGUCUGAGGCA hsa-mir-2332 pending 1194
gaaUagaaagAAUGUGGAAGUGGUCUGAGGCAUaUagag UaUaUgccaagaacacUaccaUa 901
GCAAAGUGAUGAGUAAUACUGG hsa-mir-2333 hsa-mir- 1195
acagUaacUUUUaUUcUcaUUUUccUUUUcUcUaccUUgU 3609
agagaaGCAAAGUGAUGAGUAAUACUGGcUgg 902 UGGCGGCGGUAGUUAUGGGCUU
hsa-mir-2334 hsa-mir- 1196 UggUGGCGGCGGUAGUUAUGGGCUUcUcUUUcUcacca
4467 gcagccccUgggccgccgccUcccU 903 AGAGCAGAAGGAUGAGAU hsa-mir-2335
hsa-mir- 1197 agUcUUcUccUggggcUUUggUggcUaUggUUgacUgggccac 4468
UcAGAGCAGAAGGAUGAGAUg 904 UGGAAUGGCCUGAAGGUGGA hsa-mir-2336 pending
1198 ccUggcagcccUcUggccUagUUcccaccacacaUgaggUggUG
GAAUGGCCUGAAGGUGGAacaga 905 GCUCCCUCUAGGGUCGCUCGGA hsa-mir-2337
hsa-mir- 1199 ccgacgcggagagcggcUcUaggUgggUUUggcggcggcgaggaca 4469
ccgccGCUCCCUCUAGGGUCGCUCGGAgcgUga 906 UGGCAAACGUGGAAGCCGAGA
hsa-mir-2338 hsa-mir- 1200
cgagccUcUUUcggcUUUccagUUUgUcUcggUccUUUggaa 4470
cgUGGCAAACGUGGAAGCCGAGAgggcUcU 907 UUUGUAUGGAUAUGUGUGUGUA
hsa-mir-2339 hsa-mir- 1201
aUaUacaUacaUgUacacacacaUgUcaUccacacacaUacaUa 3149
UaUaUaUgUUUGUAUGGAUAUGUGUGUGUAUgUgUg UgUaUac 908
UGAGGAGAUCGUCGAGGUUGGC hsa-mir-2340 hsa-mir- 1202
aaagcaggccaaccUcgaggaUcUccccagccUUggcgUUcaggU 3150b
gcUGAGGAGAUCGUCGAGGUUGGCcUgcUUc 909 UGGGAACUUAGUAGAGGUUUAA
hsa-mir-2341 hsa-mir- 1203
ccaaaUUUaaaacUUaaaccUcUacUaagUUUccaUgaaaaga 4471
acccaUGGGAACUUAGUAGAGGUUUAAgUUUUaaaUUU ga 910 GGUGGGGGGUGUUGUUUU
hsa-mir-2342- hsa-mir- 1204
UggcagacccUUgcUcUcUcacUcUcccUaaUggggcUgaagac 1 4472-1
agcUcaggggcagGGUGGGGGGUGUUGUUUUUgUUU 911 GGUGGGGGGUGUUGUUUU
hsa-mir-2342- hsa-mir- 1205 UggUgggGGUGGGGGGUGUUGUUUUUgUUUUUgagaca
2 4472-2 gagUcUUgcUccgUcgcccaggccggagU 912 CUAGUGCUCUCCGUUACAAGUA
hsa-mir-2343 hsa-mir- 1206
aaggaacaggggacacUUgUaaUggagaacacUaagcUaUggac 4473
UgcUaUggacUgCUAGUGCUCUCCGUUACAAGUAUcccc UgUUaccU 913
CACUUGUAAUGGAGAACACUAA hsa-mir-2344 pending 1207
aaaggaacaggggaCACUUGUAAUGGAGAACACUAAgcUa
UggacUgcUaUggacUgcUagUgcUcUccgUUacaagUaUccc cUgUUaccUUg 914
UUGUGGCUGGUCAUGAGGCUAA hsa-mir-2345 hsa-mir- 1208
UUgccUaccUUgUUagUcUcaUgaUcagacacaaaUaUggcUc 4474
UUUGUGGCUGGUCAUGAGGCUAAcaaggUaggcac 915 CCCUGGGGUUCUGAGGACAUG
hsa-mir-2346 pending 1209 aUgcUgCCCUGGGGUUCUGAGGACAUGcUcUgacUcccc
UgaUgUccUcUgUUccUcaggUgcUgggcga 916 CAAGGGACCAAGCAUUCAUUAU
hsa-mir-2347 hsa-mir- 1210
aUcUcaaUgagUgUgUggUUcUaaaUgacUcaUagUCAAGG 4475 GACCAAGCAUUCAUUAUgaa
917 CAGGAAGGAUUUAGGGACAGGC hsa-mir-2348 hsa-mir- 1211
aaaagccUgUcccUaagUcccUcccagccUUccagagUUggUgc 4476
CAGGAAGGAUUUAGGGACAGGCUUUg 918 CUAUUAAGGACAUUUGUGAUUC hsa-mir-2349
hsa-mir- 1212 UccUccUcccaUcaaUcacaaaUgUccUUaaUggcaUUUaagg 4477a
aUUgCUAUUAAGGACAUUUGUGAUUCacgggaggaggU 919 AUUAAGGACAUUUGUGAUUGAU
hsa-mir-2350 hsa-mir- 1213
accUccUcccgUgaaUcacaaaUgUccUUaaUagcaaUccUUaa 4477b
aUgccAUUAAGGACAUUUGUGAUUGAUgggaggagga 920 AAAAGGCAUAAAACCAAGACA
hsa-mir-2351- hsa-mir- 1214 cUUUUUUUgUUgcUUgUcUUggUUUUaUgccUUUUaUg
1 3910-2 UgccUUgaUaUAAAAGGCAUAAAACCAAGACAagcaacag aaaaac 921
AAAAGGCAUAAAACCAAGACA hsa-mir-2351- hsa-mir- 1215
cUgUcagUUUUUcUgUUgcUUgUcUUggUUUUaUgccUU 2 3910-1
UUaUaUcaaggcacaUAAAAGGCAUAAAACCAAGACAagc aacaa 922
GAGGCUGAGCUGAGGAG hsa-mir-2352 hsa-mir- 1216
ggccGAGGCUGAGCUGAGGAGccUccaaaccUgUagacagg 4478
gUcaUgcagUacUaggggcgagccUcaUccccUgcagcccUggcc 923
CUGGGAGGUGUGAUAUUGUGGU hsa-mir-2353 hsa-mir- 1217
gggaggUgUgaUaUcgUggUUccUgggaggUgUgaUaUcgUg 3689c
gUUcCUGGGAGGUGUGAUAUUGUGGUUccU 924 UAAAAACUGCAAUUACUUUC
hsa-mir-2354- hsa-mir- 1218
aUgccaaaUaUUaggUUggcacaaaagUaaUUgUggcUUUUg 1 548x-2
ccaUUaaaagUaaUggUAAAAACUGCAAUUACUUUCgUgc caaccUaaUaUUUgUgUg 925
UAAAAACUGCAAUUACUUUC hsa-mir-2354- hsa-mir- 1219
agUgcaaaagUaaUUgcagUUUUUgcgUUacUUUcaaUcgU 2 548x-1
AAAAACUGCAAUUACUUUCacacc 926 UGUGAUAUCAUGGUUCCUGGGA hsa-mir-2355-
hsa-mir- 1220 gcUcccUgggaggUGUGAUAUCAUGGUUCCUGGGAggUg 1 3689a
UgaUccUgUgcUUccUgggaggUgUgaUaUcgUggUUccUgg gagg 927
GGGAGGUGUGAUCUCACACUCG has-mir-2356- hsa-mir- 1221
UGGGAGGUGUGAUCUCACACUCGcUgggaggUgUgcUaU 1 3689d-1
cgUcUUccccgggaggUgUgaUccUgUUcUUccUg 928 CUGGGAGGUGUGAUAUUGUGGU
mir-2355-2* has-mir- 1222 gggaggUGUGAUAUCAUGGUUCCUGGGAggUgUgaUccc
3689b-1 gUgcUUcCUGGGAGGUGUGAUAUUGUGGUUccU 929
UGUGAUAUCAUGGUUCCUGGGA hsa-mir-2355- hsa-mir- 1223
gggaggUGUGAUAUCAUGGUUCCUGGGAggUgUgaUccc 2 3689b-1
gUgcUUcCUGGGAGGUGUGAUAUUGUGGUUccU 930 GGGAGGUGUGAUCUCACACUCG
has-mir-2356- hsa-mir- 1224 acUGGGAGGUGUGAUCUCACACUCGcUgggaggUgUgcU
2 3689d-2 aUcgUcUUcccUgggaggUgUgaUccUgUUcUUccUgagcg 931
UGUGAUAUCAUGGUUCCUGGGA hsa-mir-2355- has-mir- 1225
gggaggUGUGAUAUCAUGGUUCCUGGGAggUaUgaUaUc 3 3689b-2
gUggUUccUgggaggUgUgaUcccgUgcUcccU 932 UGUGAUAUCGUGCUUCCUGGGA
hsa-mir-2355b hsa-mir- 1226 aggUGUGAUAUCGUGCUUCCUGGGAcgUgUgaUgcUgUg
3689f cUUccUgggaggUgUgaUcccacacUc 933 CGCGCGGCCGUGCUCGGAGCAG
hsa-mir-2356 hsa-mir- 1227
gaaaccaagUccgagcgUggcUggcgcgggaaagUUcgggaacgC 4479
GCGCGGCCGUGCUCGGAGCAGcgcca 934 CCAGGCUCUGCAGUGGGAACU hsa-mir-2357a
hsa-mir- 1228 ccUgUUccgggcaUcaccUcccacUgcagagccUggggagccggac 3155a
agcUcccUUcCCAGGCUCUGCAGUGGGAACUgaUgccUgg aacagU 935
CCAGGCUCUGCAGUGGGA hsa-mir-2357b hsa-mir- 1229
ccacUgcagagccUgggaagggagcUgUccggcUccCCAGGCUC 3155b UGCAGUGGGAgU 936
AAAAGUAACUGCGGUUUUUGA hsa-mir-2358 hsa-mir- 1230
gUgcAAAAGUAACUGCGGUUUUUGAgaagUaaUUgaaaa 548ak ccgcaaUUacUUUUgcag
937 AGCCAAGUGGAAGUUACUUUA hsa-mir-2359 hsa-mir- 1231
gcagaggUgagUUgaccUccacagggccacccagggagUaagUAG 4480
CCAAGUGGAAGUUACUUUAccUcUgU 938 GGAGUGGGCUGGUGGUU hsa-mir-2360
hsa-mir- 1232 GGAGUGGGCUGGUGGUUUUUUaagaggaagggagaccUa 4481
agcUagcacaUgagcacgcUc 939 GAGCGAUCCGAGGGACUG hsa-mir-2361 pending
1233 cggcUUcccgcggUccccggUgcUgaggagaGAGCGAUCCGAG GGACUGcgccgcc 940
AAGGGCUUCCUCUCUGCAGGAC hsa-mir-2362- hsa-mir- 1234
acaUUaUUcaggccggUccUgcagagaggaagcccUUccaaUacc 1 3158-2
UgUaagcagAAGGGCUUCCUCUCUGCAGGACcggccUgaa
UaaUga 941 AAGGGCUUCCUCUCUGCAGGAC hsa-mir-2362- hsa-mir- 1235
ggaUcaUUaUUcaggccggUccUgcagagaggaagcccUUcUgc 2 3158-1
UUacaggUaUUggAAGGGCUUCCUCUCUGCAGGACcggcc UgaaUaaUgUaaUca 942
AACCCAGUGGGCUAUGGAAAUG hsa-mir-2363 hsa-mir- 1236
agUgagcAACCCAGUGGGCUAUGGAAAUGUgUggaagaU 4482
ggcaUUUcUaUUUcUcagUggggcUcUUacc 943 GGGGUGGUCUGUUGUUG hsa-mir-2364
hsa-mir- 1237 aaaaaacaacaUacUUagUgcaUacccaUaUaaUaUUaGGGG 4483
UGGUCUGUUGUUGUUUUUcU 944 AAAAGGCGGGAGAAGCCCCA hsa-mir-2365 hsa-mir-
1238 gggUUUccUcUgccUUUUUUUccaaUgaaaaUaacgaaaccU 4484
gUUaUUUcccaUUgagggggaAAAAGGCGGGAGAAGCCCC A 945
UGGCUGUUGGAGGGGGCAGGCU hsa-mir-2366 pending 1239
ggagccagcccUccUcccgcacccaaacUUggagcacUUgaccUU
UGGCUGUUGGAGGGGGCAGGCUcg 946 UAACGGCCGCGGUACCCUAA hsa-mir-2367
hsa-mir- 1240 agaggcaccgccUgcccagUgacaUgcgUUUAACGGCCGCGG 4485
UACCCUAAcUgUgca 947 UAGGAUUACAAGUGUCGGCCAC hsa-mir-2368 hsa-mir-
1241 ccUAGGAUUACAAGUGUCGGCCACgggcUgggcacagUggc 3159
UcacgccUgUaaUcccagc 948 GCUGGGCGAGGCUGGCA hsa-mir-2369 hsa-mir-
1242 gcaUGCUGGGCGAGGCUGGCAUcUagcacaggcggUagaUg 4486
cUUgcUcUUgccaUUgcaaUga 949 AGAGCUGGCUGAAGGGCAG hsa-mir-2370
hsa-mir- 1243 acUgUccUUcagccAGAGCUGGCUGAAGGGCAGaagggaac 4487
UgUccUUcagccagagcUggcUgaagggcaga 950 AGGGGGCGGGCUCCGGCG
hsa-mir-2371 hsa-mir- 1244
ggUAGGGGGCGGGCUCCGGCGcUgggaccccacUagggUgg 4488
cgccUUggccccgccccgccc 951 GCCGAGAGUCGUCGGGGUU hsa-mir-2372 pending
1245 UcGCCGAGAGUCGUCGGGGUUUccUgcUUcaacagUgcU Uggacggaacccggcgc 952
UGGGGCUAGUGAUGCAGGACG hsa-mir-2373 hsa-mir- 1246
gggggUGGGGCUAGUGAUGCAGGACGcUggggacUggaga 4489
agUccUgccUgacccUgUccca 953 UGUGACUUUAAGGGAAAUGGCG hsa-mir-2374
hsa-mir- 1247 UggaaacUGUGACUUUAAGGGAAAUGGCGcacagcagacc 3164
cUgcaaUcaUgccgUUUUgcUUgaagUcgcagUUUccc 954 UCUCAGGAGUAAAGACAGAGUU
hsa-mir-2375 hsa-mir- 1248
aacUUgaaggUagggaacUcUgUcUUcacUcaUgagUaccUUc 3664
caacacgagcUCUCAGGAGUAAAGACAGAGUUcccUaccU UcaaUgU 955
AGGUGGAUGCAAUGUGACCUCA hsa-mir-2376 hsa-mir- 1249
caAGGUGGAUGCAAUGUGACCUCAacUcUUggUccUcUg 3165 aggUcacaUUgUaUccaccUUa
956 AACGGCAAUGACUUUUGUACCA hsa-mir-2377 hsa-mir- 1250
ggUcggUgcaaaagUaaUUgcUgUUUUUgccaUUaaaaaUaa 548a1
UggcaUUaaaagUaaUggcaaaAACGGCAAUGACUUUUGU ACCAaUcUaaUaUcU 957
UCUGGUAAGAGAUUUGGGCAUA hsa-mir-2378 hsa-mir- 1251
aUagUUUcUgcaaUgcUcaaaUcUcUggccaaagaccagaacU 4490
UaaUggUcUCUGGUAAGAGAUUUGGGCAUAUUagaaac Uaa 958
AAUGUGGACUGGUGUGACCAAA hsa-mir-2379- hsa-mir- 1252
acaUUUggUcacaccagUccacaUUaacgUggaccagacaaUaU 1 4491
UAAUGUGGACUGGUGUGACCAAAa 959 AAUGUGGACUGGUGUGACCAAA hsa-mir-2379-
pending 1253 accUggacaUUUggUcacaccagUccacaUUaacgUggaccagac 2
aaUaUUAAUGUGGACUGGUGUGACCAAAagUccaggc 960 GGGGCUGGGCGCGCGCC
hsa-mir-2380 hsa-mir- 1254
cUgcagcgUgcUUcUccaggccccgcgcgcggacagacacacgga 4492
caagUcccgccaGGGGCUGGGCGCGCGCCagccgg 961 AGAAGGCCUUUCCAUCUCUGU
hsa-mir-2381 hsa-mir- 1255
ccagagaUgggaaggccUUccggUgaUUaUcacagccaUgccUU 4493
UaccUccAGAAGGCCUUUCCAUCUCUGUc 962 CCAGACUGUGGCUGACCAGAGG
hsa-mir-2382 hsa-mir- 1256
agUUUUagUUacccUggUcaUcUgcagUcUgaaaaUacaaaa 4494
UggaaaaUUCCAGACUGUGGCUGACCAGAGGUaacUgaa acc 963 AGGAGAAGCAGGAGCUGU
hsa-mir-2383 pending 1257 cUgAGGAGAAGCAGGAGCUGUcUUggUacaUUcaggUcac
Ug 964 AAUGUAAACAGGCUUUUUGCU hsa-mir-2384 hsa-mir- 1258
aagaAAUGUAAACAGGCUUUUUGCUcagUggagUUaUUU 4495
UgagcaaaaagcUUaUUUacaUUUcUg 965 GAGGAAACUGAAGCUGAGAGGG hsa-mir-2385
hsa-mir- 1259 ACAUCAGCUCAUAUAAUCCUCGAAGCUGCCUUUAGAA 4496
AUGAGGAAACUGAAGCUGAGAGGG 966 CUCCGGGACGGCUGGGC hsa-mir-2386
hsa-mir- 1260 acCUCCGGGACGGCUGGGCgccggcggccgggagaUccgcgcU 4497
UccUgaaUcccggccggcccgcccggcgcccgUccgcccgcgggUc 967
UGGGCUGGCAGGGCAAGUGCUG hsa-mir-2387 hsa-mir- 1261
agggcUGGGCUGGCAGGGCAAGUGCUGcagaUcUUUgUc 4498
UaagcagccccUgccUUggaUcUccca 968 GAGGCUGAAGGAAGAUGG hsa-mir-2388
hsa-mir- 1262 cUcaggcUcagUggUgcaUgcUUaUagUcccagccacUcUgGA 4419b
GGCUGAAGGAAGAUGGcUUgagccU 969 AAGACUGAGAGGAGGGA hsa-mir-2389
hsa-mir- 1263 AAGACUGAGAGGAGGGAacUggUgagUUgUacaUagaaa 4499
UgcUUUcUaacUccUUgUcUcagUcUgUUU 970 UGAGGUAGUAGUUUCUU hsa-mir-2390
hsa-mir- 1264 caggagagaaagUacUgcccagaagcUaaagUgUagaUcaaacgc 4500
aUaaUggcUGAGGUAGUAGUUUCUUgaacUU 971 CGGCUGGGAGCCGAGGCGUCGG
hsa-mir-2391 pending 1265 ggCGGCUGGGAGCCGAGGCGUCGGUgcagaccUggagacg
ggcaUgggggggcUgcggcUgcUggcUgUg 972 UAUGUGACCUCGGAUGAAUCA
hsa-mir-2392 hsa-mir- 1266 UAUGUGACCUCGGAUGAAUCAcUgaaaUaUgUcUgagcU
4501 UcUgUUUcaUcagaUgUcacaUUUU
agccUUUagcaagUUgUaaUcUUUUUgcUgaUggagggUcU 973
GCUGAUGAUGAUGGUGCUGAAG hsa-mir-2393 hsa-mir- 1267
UgccUccaUggggaUgGCUGAUGAUGAUGGUGCUGAAGg 4502 c 974
AGAUGUAUGGAAUCUGUAUAU hsa-mir-2394 hsa-mir- 1268
gAGAUGUAUGGAAUCUGUAUAUaUcUaUaUaUaUgUgU 3171 aUaUaUagaUUccaUaaaUcUa
975 UUUAAGCAGGAAAUAGAAUUUA hsa-mir-2395 hsa-mir- 1269
acaaUgUagaUaUUUAAGCAGGAAAUAGAAUUUAcaUaU 4503
aaaUUUcUaUUUgUUUcUaUUUccUgcUUaaaUaUcUaca UUgc 976
UGUGACAAUAGAGAUGAACAUG hsa-mir-2396 hsa-mir- 1270
cUaagaUaaUgUccUccaggUUcaUcUcUgUUgUcaUUUgU 4504
ggcaUggaccaUUUGUGACAAUAGAGAUGAACAUGgagga UaUUaUcUUaa 977
AGGCUGGGCUGGGACGGA hsa-mir-2397 hsa-mir- 1271
ggAGGCUGGGCUGGGACGGAcacccggccUccacUUUcUg 4505
UggcaggUaccUccUccaUgUcggcccgccUUg 978 AAAUGGGUGGUCUGAGGCAA
hsa-mir-2398 hsa-mir- 1272
UggccUcUgccaUcagaccaUcUgggUUcaagUUUggcUccaU 4506
cUUUaUgAAAUGGGUGGUCUGAGGCAAgUggUcU 979 UAGGAUGGGGGUGAGAGGUG
hsa-mir-2399 hsa-mir- 1273
aUggUcccUcccaaUccagccaUUccUcagaccaggUggcUcccg 2392
agccaccccaggcUgUAGGAUGGGGGUGAGAGGUGcUag 980 CUGGGUUGGGCUGGGCUGGG
hsa-mir-2400 hsa-mir- 1274
UcUgggcUgagccgagcUgggUUaagccgagCUGGGUUGGGC 4507 UGGGCUGGGU 981
AAGGGACUGGAGUGGAUUGGGU hsa-mir-2401 pending 1275
ggAAGGGACUGGAGUGGAUUGGGUacaUcUaUUaUagU
gggagcaccUacUacaacccgUcccUcaagagUcgagUcacc 982
AAGGGGCUGGAGUGGAUUGGGG hsa-mir-2402 pending 1276
cUggUgaaUcUgggUccgccagcccccagggAAGGGGCUGGA GUGGAUUGGGGaaaUccaU 983
GCGGGGCUGGGCGCGCG hsa-mir-2403 hsa-mir- 1277
aggacccaGCGGGGCUGGGCGCGCGgagcagcgcUgggUgca 4508
gcgccUgcgccggcagcUgcaagggccg 984 ACUAAAGGAUAUAGAAGGUUUU
hsa-mir-2404- hsa-mir- 1278 cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
1 4509-1 UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU gagaUagUaUUagaa
985 ACUAAAGGAUAUAGAAGGUUUU hsa-mir-2404- hsa-mir- 1279
cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU 2 4509-2
UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU gagaUagUaUUagaa 986
ACUAAAGGAUAUAGAAGGUUUU hsa-mir-2404- hsa-mir- 1280
cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU 3 4509-3
UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU gagaUagUaUUagaa 987
AAGCAAUACUGUUACCUGAAAU hsa-mir-2405 hsa-mir- 1281
cUcaaagAAGCAAUACUGUUACCUGAAAUaggcUgcgaaga 3942
UaacagUaUUUcagaUaacagUaUUacaUcUUUgaa 988 UGAGGGAGUAGGAUGUAUGGUU
hsa-mir-2406 hsa-mir- 1282 gUgUaUgUGAGGGAGUAGGAUGUAUGGUUgUUagaUa
4510 gacaacUacaaUcUUUUcUcacaacagacag 989 GAAGAACUGUUGCAUUUGCCCU
hsa-mir-2407 hsa-mir- 1283 aaaaaaaagggaaaGAAGAACUGUUGCAUUUGCCCUgcac
4511 UcagUUUgcacagggUaaaUgcaaUagUUcUUcUUUcccUU UUUUUa 990
CAGGGCCUCACUGUAUCGCCCA hsa-mir-2408 hsa-mir- 1284
cUcagcccgggcaaUaUagUgagaccUcgUcUcUacaaaaaaUU 4512
gagaCAGGGCCUCACUGUAUCGCCCAggcUgga
aUUcUaggUggggAGACUGACGGCUGGAGGCCCAUaagc 991 AGACUGACGGCUGGAGGCCCAU
hsa-mir-2409 hsa-mir- 1285
UgUcUaaaacUUcggcccccagaUUUcUggUcUccccacUUcag 4513 aac 992
ACAGGCAGGAUUGGGGAA hsa-mir-2410 hsa-mir- 1286
gUUgagACAGGCAGGAUUGGGGAAacaUcUUUUaccUcg 4514 UcUcUUgccUgUUUUaga 993
AGGACUGGACUCCCGGCAGCCC hsa-mir-2411 hsa-mir- 1287
gcgggaggUgUaacAGGACUGGACUCCCGGCAGCCCcaggg 4515
caggggcgUggggagcUggUccUagcUcagcgcUcccgga 994 UAGUGAGUUAGAGAUGCAGAGC
hsa-mir-2412 hsa-mir- 1288 agcgUUaccUggUAGUGAGUUAGAGAUGCAGAGCccUgg
3174 gcUccUcagcaaaccUacUggaUcUgca 995 ACUGGCCUGGGACUACCGGGGG
hsa-mir-2413 hsa-mir- 1289
UcUgcagcUcccggcagccUcgggccacacUcccgggaUccccag 3176
ggACUGGCCUGGGACUACCGGGGGUggcggc 996 GGGAGAAGGGUCGGGGC hsa-mir-2414
hsa-mir- 1290 aGGGAGAAGGGUCGGGGCagggagggcagggcaggcUcUgg 4516
ggUggggggUcUgUgagUcagccacggcUcUgcccacgUcUcccc 997
UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1291
agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc 1 3180-1
gUGGGGCGGAGCUUCCGGAGGCCcc 998 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415-
hsa-mir- 1292 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc 2
3180-2 gUGGGGCGGAGCUUCCGGAGGCCcc 999 UGGGGCGGAGCUUCCGGAGGCC
hsa-mir-2415- hsa-mir- 1293
agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc 3 3180-3
gUGGGGCGGAGCUUCCGGAGGCCcc
1000 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1294
agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc 4 3180-4
gUGGGGCGGAGCUUCCGGAGGCCcc 1001 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415-
hsa-mir- 1295 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc 5
3180-5 gUGGGGCGGAGCUUCCGGAGGCCcc 1002 CUCGUGGGCUCUGGCCACGGC
hsa-mir-2416 hsa-mir- 1296
UggagggcaUUaggcagUggccagagcccUgcagUgcUgggcaUg 3677
ggcUUCUCGUGGGCUCUGGCCACGGCccUgagcUccUcc 1003 AGAAGGGGUGAAAUUUAAACGU
hsa-mir-2417- hsa-mir- 1297
gccaggaUcacagacgUUUaaaUUacacUccUUcUgcUgUgcc 1 3179-1
UUacagcagUAGAAGGGGUGAAAUUUAAACGUcUgUga UccUggg 1004
AGAAGGGGUGAAAUUUAAACGU hsa-mir-2417- hsa-mir- 1298
gaUcacagacgUUUaaaUUacacUccUUcUgcUgUgccUUaca 2 3179-2
gcagUAGAAGGGGUGAAAUUUAAACGUcUgUgaUccUgg g 1005
AGAAGGGGUGAAAUUUAAACGU hsa-mir-2417- hsa-mir- 1299
gccaggaUcacagacgUUUaaaUUacacUccUUcUgcUgUgcc 3 3179-3
UUacagcagUAGAAGGGGUGAAAUUUAAACGUcUgUga UccUgg 1006
AAAUAUGAUGAAACUCACAGCUG hsa-mir-2418 hsa-mir- 1300
aggUAAAUAUGAUGAAACUCACAGCUGAGgagcUUagca AG 4517
agUagcUaaggccagagcUUgUgUUUgggUggUgUggcUg 1007
GCUCAGGGAUGAUAACUGUGCUG hsa-mir-2419 hsa-mir- 1301
UgggggaaaagUgcUgggaUUgaUUagUgaUgUcUgcUgggga AGA 4518
accgggGCUCAGGGAUGAUAACUGUGCUGAGAagcccccU 1008 CAGCAGUGCGCAGGGCUG
hsa-mir-2420 hsa-mir- 1302
aaccUCAGCAGUGCGCAGGGCUGcacUgUcUccgUcUgcgg 4519 ccUgcagUaagcgggUa
1009 UUGGACAGAAAACACGCAGGAA hsa-mir-2421 hsa-mir- 1303
gUgUgccaccUgcgUgUUUUcUgUccaaaUcagaaaaggaUU 4520
UGGACAGAAAACACGCAGGAAgaaggaa 1010 GCUAAGGAAGUCCUGUGCUCAG
hsa-mir-2422 hsa-mir- 1304 UcgGCUAAGGAAGUCCUGUGCUCAGUUUUgUagcaUca
4521 aaacUaggaUUUcUcUUgUUac 1011 CUGGACUGAGCCAUGCUACUGG
hsa-mir-2423 hsa-mir- 1305 UgaggUUUCUGGACUGAGCCAUGCUACUGGcUUcUcUg
1269b gUUcUccagcUUacagaUggcUUaUcaUgggaccUcU 1012
UGACUCUGCCUGUAGGCCGGU hsa-mir-2424 hsa-mir- 1306
gcgggcgUUgccUgggggccUcgcagggggagaUccagcccaggcU 4522
ggUUccgcUGACUCUGCCUGUAGGCCGGUggcgUcUUcU gg 1013
GACCGAGAGGGCCUCGGCUGU hsa-mir-2425 hsa-mir- 1307
gcggggGACCGAGAGGGCCUCGGCUGUgUgaggacUagagg 4523
cggccgaggcccgggccggUUcccccga 1014 UAGAGGCUGGAAUAGAGAUUCU
hsa-mir-2426 pending 1308 aUUUUAGAGGCUGGAAUAGAGAUUCUUgaggcUUggaa
gagUaaggaUcccUUUaUcUgUccUcUaggag 1015 UAGCCUUCAGAUCUUGGUGUUU
hsa-mir-2427 hsa-mir- 1309
UggaUcUgaaggcUgccccUUUgcUcUcUggggUAGCCUUCA 3614 GAUCUUGGUGUUUU 1016
CCACUUGGAUCUGAAGGCUGCC hsa-mir-2427* has-mir- 1310
UgggCCACUUGGAUCUGAAGGCUGCCccUUUgcUcUcUg 3614
gggUagccUUcagaUcUUggUgUUUU 1017 AUAGCAGCAUGAACCUGUCUCA hsa-mir-2428
hsa-mir- 1311 gaacgAUAGCAGCAUGAACCUGUCUCAcUgcagaaUUaUU 4524
UUgagacaggcUUaUgcUgcUaUccUUca 1018 UGAGACAGGCUUAUGCUGCUAU
hsa-mir-2428* has-mir- 1312
ggaacgaUagcagcaUgaaccUgUcUcacUgcagaaUUaUUUU 4524
GAGACAGGCUUAUGCUGCUAUccUUca 1019 GGGGGGAUGUGCAUGCUGGUU hsa-mir-2429
hsa-mir- 1313 gUcagaGGGGGGAUGUGCAUGCUGGUUggggUgggcUgc 4525
cUgUggaccaaUcagcgUgcacUUccccacccUgaa 1020 CAGGGAGGUGAAUGGUUCUGUC
hsa-mir-2430 pending 1314 cUUagcUcccUggcUUcagcccUUUUUcCAGGGAGGUGAA
UGGUUCUGUCUcgc 1021 GCUGACAGCAGGGCUGGCCGCU hsa-mir-2431 hsa-mir-
1315 UgcggUgacaUcagggcccagUcccUgcUgUcaUgccccaggUga 4526
cgUgcUggGCUGACAGCAGGGCUGGCCGCUaacgUcacUg Uc 1022
UGGUCUGCAAAGAGAUGACUGU hsa-mir-2432 hsa-mir- 1316
ccagaagUGGUCUGCAAAGAGAUGACUGUgaaUccaagaU 4527
ccacaUcagcUcUgUgcUgccUacaUcUga 1023 UCAUUAUAUGUAUGAUCUGGAC
hsa-mir-2433 hsa-mir- 1317
UaUUcUacUgagagUacagaUcUUUaUaUaUaUgaUcaUUa 4528
UaUgUaUgaUgagaUCAUUAUAUGUAUGAUCUGGACacc cagUagaaUc 1024
AUUGGACUGCUGAUGGCCCGU hsa-mir-2434 hsa-mir- 1318
aUgacaggccaUcagcagUccaaUgaagacaUgaagacccaaUgU 4529
cUUcAUUGGACUGCUGAUGGCCCGUcacUggga 1025 UUGGAGGGUGUGGAAGACAUC
hsa-mir-2435 pending 1319 cUccacaUUGGAGGGUGUGGAAGACAUCUgggccaacUcU
gaUcUcUUcaUcUaccccccaggacUggga 1026 CCCAGCAGGACGGGAGCG hsa-mir-2436
hsa-mir- 1320 cgaccgcacccgcccgaagcUgggUcaaggagCCCAGCAGGACG 4530
GGAGCGcggcgc 1027 AUGGAGAAGGCUUCUGA hsa-mir-2437 hsa-mir- 1321
gccUaggagUccUUggUcagUggggacAUGGAGAAGGCUUC 4531 UGAgga 1028
AAAAGCUGGGUUGAGAA hsa-mir-2438 hsa-mir- 1322
gccUUcUcUUcccagUUcUUccUggagUcggggAAAAGCUG 320e GGUUGAGAAggUgaaaaga
1029 UGGAAGGUAGACGGCCAGAGAG hsa-mir-2439 hsa-mir- 1323
ggUcaccUgUcUggccagcUacgUccccacggcccUUgUcagUg 3190
UGGAAGGUAGACGGCCAGAGAGgUgaccc 1030 UCUGGGAGGUUGUAGCAGUGGA
hsa-mir-2440 hsa-mir- 1324 aggaagggaUUCUGGGAGGUUGUAGCAGUGGAaaaagUU
3192 cUUUUcUUccUcUgaUcgcccUcUcagcUcUUUccUUcUg 1031
CCCCGGGGAGCCCGGCG hsa-mir-2441 hsa-mir- 1325
acagaCCCCGGGGAGCCCGGCGgUgaagcUccUggUaUccU 4532 gggUgUcUga 1032
UGGAAGGAGGUUGCCGGACGCU hsa-mir-2442 hsa-mir- 1326
UgagaaUgUGGAAGGAGGUUGCCGGACGCUgcUggcUgc 4533
cUUccagcgUccacUUcccUUUcUcUcUcUcc 1033 CAAGAACCUCAAUUACCUUUGC
hsa-mir-2443 pending 1327
cggUUggUgcaaaggUaaUUgUggUUUcUgccaUUgaaagUa
aaggCAAGAACCUCAAUUACCUUUGCagcgaccU 1034 AAUCUGAGAAGGCGCACAAGGU
hsa-mir-2444 has-mir- 1328 UcgagggAAUCUGAGAAGGCGCACAAGGUUUgUgUccaa
3200 UacagUccacaccUUgcgcUacUcaggUcUgcUcgUg 1035
CACCUUGCGCUACUCAGGUCUG hsa-mir-2444* hsa-mir- 1329
UcgagggaaUcUgagaaggcgcacaaggUUUgUgUccaaUacag 3200
UccaCACCUUGCGCUACUCAGGUCUGcUcgU 1036 GGAGGAACCUUGGAGCUUCGGC
hsa-mir-2445 hsa-mir- 1330
ggcUgaagcUcUaaggUUccgccUgcgggcaggaagcGGAGGA 3928 ACCUUGGAGCUUCGGCa
1037 GGAUGGAGGAGGGGUCU hsa-mir-2446 hsa-mir- 1331
UgUgaaUgacccccUUccagagccaaaaUcaccagGGAUGGAG 4534 GAGGGGUCUUgggUacU
1038 ACUGGACUAGGAGUCAGAAGG hsa-mir-2447 hsa-mir- 1332
gggagcACUGGACUAGGAGUCAGAAGGUggagUUcUggg 378i
UgcUgUUUUcccacUcUUgggcccUgggcaUgUUcUg 1039 GUGGACCUGGCUGGGAC
hsa-mir-2448 hsa-mir- 1333
aacUgggUcccagUcUUcacagUUggUUUcUgacacGUGGAC 4535 CUGGCUGGGACgaUgUg
1040 ACCUGGACCCAGCGUAGACAAA hsa-mir-2449 hsa-mir- 1334
ccACCUGGACCCAGCGUAGACAAAgaggUgUUUcUacUcc 3690 aUaUcUaccUggacccagUgU
1041 CAAAAACUGCAGUUACUUUUGU hsa-mir-2450 hsa-mir- 1335
agUUggUgcaaaagUaaUUgcggUUUUUgccgUcgaaaaUaa 548am
UggCAAAAACUGCAGUUACUUUUGUaccaaUg 1042 UUGGGCUGGGCUGGGUUGGG
hsa-mir-2451 hsa-mir- 1336 UUUGGGCUGGGCUGGGUUGGGcagUUcUUcUgcUggac
1587 UcaccUgUgaccagc 1043 UGUGGUAGAUAUAUGCACGAU hsa-mir-2452
hsa-mir- 1337 aUgUgUggUagaUaUaUgcacUgUaUaUaaacaUaaUGUGG 4536
UAGAUAUAUGCACGAUaUag 1044 AGAAGGGAAAGAACAUCAA hsa-mir-2453 pending
1338 aUggUgUUUgccUccUUcaUccgcaaggcaUcUgaUgcccacga
agUUaggaaggUccUUggggAGAAGGGAAAGAACAUCAAa 1045 CUGUCCUAAGGUUGUUGAGUU
hsa-mir-2454 hsa-mir- 1339
cgcaUgacUcUUcaaccUcaggacUUgcagaaUUaaUggaaUg 676
CUGUCCUAAGGUUGUUGAGUUgUgca 1046 AAAAGGCAUUGUGGUUUUUG hsa-mir-2455
hsa-mir- 1340 caUUaggUUggUgcAAAAGGCAUUGUGGUUUUUGccUa 548an
UaaaagUaaUggcaaaaaccgcaaUUccUUUUgcaccaaccUaa U 1047
CGGGCGGCGGCUGUGUUGCGCA hsa-mir-2456 pending 1341
cacUcgcgcUgcggccagcgcccgggccUgcgggccCGGGCGGCG GCUGUGUUGCGCAgUc 1048
UAUGGAAGGGAGAAGAGCUUUA hsa-mir-2457 hsa-mir- 1342
UUaUUaaUAUGGAAGGGAGAAGAGCUUUAaUgaUUgga 3202-1
gUcaUUUUcagagcaUUaaagcUcUUcUcccUUccaUaUUaa UgU 1049
CGGCGGGGACGGCGAUUGGU hsa-mir-1908 hsa-mir- 1343
UgccgCGGCGGGGACGGCGAUUGGUccgUaUgUgUggUg 1908
ccaccggccgccggcUccgccccggcc 1050 CCUCCUGCCCUCCUUGCUGUAG
hsa-mir-1976 pending 1344
gcaaagggUggcagcaaggaaggcaggggUccUaaggUgUgUCC
UCCUGCCCUCCUUGCUGUAGacUUUgg 1051 UGAGCCGAGCUGAGCUUAGCUG
hsa-mir-2457 hsa-mir- 1345 UGAGCCGAGCUGAGCUUAGCUGggcUgagcUa 4537
accagggcUgggcUgagcUgggcUgagcUgagcUgagc
GAGCUUGGAUGAGCUGGGCUGAacUgggcUgg 1052 GAGCUUGGAUGAGCUGGGCUGA
hsa-mir-2458 hsa-mir- 1346 gUUgagcUgggcUgggcUgagUUgagccaggcUgaU
4538 cUgggcUgag 1053 GCUGAACUGGGCUGAGCUGGGC hsa-mir-2459 hsa-mir-
1347 UgagcUgggcUcUgcUgUgcUgUgcUgagcagggcU 4539
gaGCUGAACUGGGCUGAGCUGGGC 1054 CUGGGCUGAAUGACAGUGAUGAG hsa-mir-2460
pending 1348 gUcaagUcagaacagccaggUagagcccUUgUccaaac
CUGGGCUGAAUGACAGUGAUGAG 1055 GGGAGCCGGGGCUGUGAGAGGA hsa-mir-2461
pending 1349 cUcUUUgagccUUggcUgccUUggUgcagcagggUc
aUcUgUagggccaccccacagcUcUUUccUUccccUc
cUcUcUccaGGGAGCCGGGGCUGUGAGAGGA 1056 UUAGUCCUGCCUGUAGGUUUA
hsa-mir-2462 hsa-mir- 1350 aagcUgcaUggaccaggacUUggcaccUUUggccUU
4540 AGUCCUGCCUGUAGGUUUA
TABLE-US-00018 TABLE 33 Primers for Reverse Transcription for a
subset of the known miRNAs identified by deep sequencing analysis.
SEQ Putative Primer for reverse SEQ ID Major/Minor Mature Sequence
transcription ID NO. miRNA ID Captured of miRNA NO. 7 hsa-mir-129-
AAGCCCUUACCCCAAAAAGCAU AAGCCCTTACCCCAAAAAGCAT 1351 2* 22
hsa-mir-25* AGGCGGAGACUUGGGCAAUUG AGGCGGAGACTTGGGCAATTG 1352 24
hsa-mir-30b* CUGGGAGGUGGAUGUUUACUUC CTGGGAGGTGGATGTTTACTTC 1353 26
hsa-mir-30c- CUGGGAGAAGGCUGUUUACUCU CTGGGAGAAGGCTGTTTACTCT 1354 2*
27 hsa-mir-30e* CUUUCAGUCGGAUGUUUACAGC CTTTCAGTCGGATGTTTACAGC 1355
29 hsa-mir-342- UCUCACACAGAAAUCGCACCCGU TCTCACACAGAAATCGCACCCGT
1356 3p 56 hsa-let-7a-2 UGAGGUAGUAGGUUGUAUAGUU
TGAGGTAGTAGGTTGTATAGTT 1357 57 hsa-let-7a-3 UGAGGUAGUAGGUUGUAUAGUU
TGAGGTAGTAGGTTGTATAGTT 1358 58 hsa-let-7c UGAGGUAGUAGGUUGUAUGGUU
TGAGGTAGTAGGTTGTATGGTT 1359 63 hsa-mir-100 AACCCGUAGAUCCGAACUUGUG
AACCCGTAGATCCGAACTTGTG 1360 64 hsa-mir-101-1 UACAGUACUGUGAUAACUGAA
TACAGTACTGTGATAACTGAA 1361 65 hsa-mir-101-2 GUACAGUACUGUGAUAACUGAA
TACAGTACTGTGATAACTGAA 1362 66 hsa-mir-103-1 AGCAGCAUUGUACAGGGCUAUGA
AGCAGCATTGTACAGGGCTATGA 1363 67 hsa-mir-103-2
AGCAGCAUUGUACAGGGCUAUGA AGCAGCATTGTACAGGGCTATGA 1364 70
hsa-mir-106a AAAAGUGCUUACAGUGCAGGUAG AAAAGTGCTTACAGTGCAGGTAG 1365
71 hsa-mir-106b UAAAGUGCUGACAGUGCAGAU TAAAGTGCTGACAGTGCAGAT 1366 72
hsa-mir-107 AGCAGCAUUGUACAGGGCUAUCA AGCAGCATTGTACAGGGCTATCA 1367 73
hsa-mir-10a UACCCUGUAGAUCCGAAUUUGUG TACCCTGTAGATCCGAATTTGTG 1368 74
hsa-mir-10b UACCCUGUAGAACCGAAUUUGUG TACCCTGTAGAACCGAATTTGTG 1369 98
hsa-mir-128-1 UCACAGUGAACCGGUCUCUUU TCACAGTGAACCGGTCTCTTT 1370 99
hsa-mir-128-2 UCACAGUGAACCGGUCUCUUU TCACAGTGAACCGGTCTCTTT 1371 100
hsa-mir-129-1 CUUUUUGCGGUCUGGGCUUGC CTTTTTGCGGTCTGGGCTTGC 1372 101
hsa-mir-129-2 CUUUUUGCGGUCUGGGCUUGC CTTTTTGCGGTCTGGGCTTGC 1373 103
hsa-mir-1295 UUAGGCCGCAGAUCUGGGUGA TTAGGCCGCAGATCTGGGTGA 1374 119
hsa-mir-140- UACCACAGGGUAGAACCACGG TACCACAGGGTAGAACCACGG 1375 3p
122 hsa-mir-143 UGAGAUGAAGCACUGUAGCUC TGAGATGAAGCACTGTAGCTC 1376
125 hsa-mir-146a UGAGAACUGAAUUCCAUGGGUU TGAGAACTGAATTCCATGGGTT 1377
126 hsa-mir-146b UGAGAACUGAAUUCCAUAGGCU TGAGAACTGAATTCCATAGGCT 1378
127 hsa-mir-148a UCAGUGCACUACAGAACUUUGU TCAGTGCACTACAGAACTTTGT 1379
130 hsa-mir-151- CUAGACUGAAGCUCCUUGAGG CTAGACTGAAGCTCCTTGAGG 1380
3p 131 hsa-mir-152 UCAGUGCAUGACAGAACUUGG TCAGTGCATGACAGAACTTGG 1381
132 hsa-mir-155 UUAAUGCUAAUCGUGAUAGGGGU TTAATGCTAATCGTGATAGGGGT
1382 133 hsa-mir-15a UAGCAGCACAUAAUGGUUUGUG TAGCAGCACATAATGGTTTGTG
1383 134 hsa-mir-15b UAGCAGCACAUCAUGGUUUACA TAGCAGCACATCATGGTTTACA
1384 135 hsa-mir-16-1 UAGCAGCACGUAAAUAUUGGCG TAGCAGCACGTAAATATTGGCG
1385 136 hsa-mir-16-2 UAGCAGCACGUAAAUAUUGGCG TAGCAGCACGTAAATATTGGCG
1386 137 hsa-mir-17 CAAAGUGCUUACAGUGCAGGUAG CAAAGTGCTTACAGTGCAGGTAG
1387 138 hsa-mir-181a- AACAUUCAACGCUGUCGGUGAGU
AACATTCAACGCTGTCGGTGAGT 1388 1 139 hsa-mir-181a-
AACAUUCAACGCUGUCGGUGAGU AACATTCAACGCTGTCGGTGAGT 1389 2 140
hsa-mir-181b- AACAUUCAUUGCUGUCGGUGGGU AACATTCATTGCTGTCGGTGGG 1390 1
141 hsa-mir-181b- AACAUUCAUUGCUGUCGGUGGGU AACATTCATTGCTGTCGGTGGG
1391 2 142 hsa-mir-181c AACAUUCAACCUGUCGGUGAGU
AACATTCAACCTGTCGGTGAGT 1392 143 hsa-mir-181d
AACAUUCAUUGUUGUCGGUGGGU AACATTCATTGTTGTCGGTGGGTT 1393 147
hsa-mir-185 UGGAGAGAAAGGCAGUUCCUGA TGGAGAGAAAGGCAGTTCCTGA 1394 150
hsa-mir-18a UAAGGUGCAUCUAGUGCAGAUAG TAAGGTGCATCTAGTGCAGATAG 1395
151 hsa-mir-191 CAACGGAAUCCCAAAAGCAGCUG CAACGGAATCCCAAAAGCAGCTG
1396 152 hsa-mir-192 CUGACCUAUGAAUUGACAGCC CTGACCTATGAATTGACAGCC
1397 153 hsa-mir-193a- UGGGUCUUUGCGGGCGAGAUGA
TGGGTCTTTGCGGGCGAGATGA 1398 5p 159 hsa-mir-196b
UAGGUAGUUUCCUGUUGUUGGG TAGGTAGTTTCCTGTTGTTGGG 1399 160 hsa-mir-197
UUCACCACCUUCUCCACCCAGC TTCACCACCTTCTCCACCCAGC 1400 163 hsa-mir-199b
ACAGUAGUCUGCACAUUGGUUA ACAGTAGTCTGCACATTGGTTA 1401 164 hsa-mir-19a
UGUGCAAAUCUAUGCAAAACUGA TGTGCAAATCTATGCAAAACTGA 1402 175
hsa-mir-20a UAAAGUGCUUAUAGUGCAGGUAG TAAAGTGCTTATAGTGCAGGTAG 1403
176 hsa-mir-20b CAAAGUGCUCAUAGUGCAGGUAG CAAAGTGCTCATAGTGCAGGTAG
1404 186 hsa-mir-221 AGCUACAUUGUCUGCUGGGUUUC
AGCTACATTGTCTGCTGGGTTTC 1405 187 hsa-mir-222 AGCUACAUCUGGCUACUGGGU
AGCTACATCTGGCTACTGGGT 1406 190 hsa-mir-23a AUCACAUUGCCAGGGAUUUCC
ATCACATTGCCAGGGATTTCC 1407 194 hsa-mir-25 CAUUGCACUUGUCUCGGUCUGA
CATTGCACTTGTCTCGGTCTGA 1408 195 hsa-mir-26a-1
UUCAAGUAAUCCAGGAUAGGCU TTCAAGTAATCCAGGATAGGCT 1409 196
hsa-mir-26a-2 UUCAAGUAAUCCAGGAUAGGCU TTCAAGTAATCCAGGATAGGCT 1410
197 hsa-mir-26b UUCAAGUAAUUCAGGAUAGGU TTCAAGTAATTCAGGATAGGT 1411
198 hsa-mir-27a UUCACAGUGGCUAAGUUCCGC TTCACAGTGGCTAAGTTCCGC 1412
203 hsa-mir-29a UAGCACCAUCUGAAAUCGGUUA TAGCACCATCTGAAATCGGTTA 1413
208 hsa-mir-30a UGUAAACAUCCUCGACUGGAAG TGTAAACATCCTCGACTGGAAG 1414
209 hsa-mir-30b UGUAAACAUCCUACACUCAGCU TGTAAACATCCTACACTCAGCT 1415
210 hsa-mir-30c-1 UGUAAACAUCCUACACUCUCAGC TGTAAACATCCTACACTCTCAGC
1416 211 hsa-mir-30c-2 UGUAAACAUCCUACACUCUCAGC
TGTAAACATCCTACACTCTCAGC 1417 212 hsa-mir-30d UGUAAACAUCCCCGACUGGAAG
TGTAAACATCCCCGACTGGAAG 1418 213 hsa-mir-30e UGUAAACAUCCUUGACUGGAAG
TGTAAACATCCTTGACTGGAAG 1419 216 hsa-mir-320a AAAAGCUGGGUUGAGAGGGCGA
AAAAGCTGGGTTGAGAGGGCGA 1420 217 hsa-mir-320b-
AAAAGCUGGGUUGAGAGGGCAA AAAAGCTGGGTTGAGAGGGCAA 1421 1 218
hsa-mir-320b- AAAAGCUGGGUUGAGAGGGCAA AAAAGCTGGGTTGAGAGGGCAA 1422 2
227 hsa-mir-331 GCCCCUGGGCCUAUCCUAGAA GCCCCTGGGCCTATCCTAGAA 1423
231 hsa-mir-33a GUGCAUUGUAGUUGCAUUGCA GTGCATTGTAGTTGCATTGCA 1424
233 hsa-mir-340 UUAUAAAGCAAUGAGACUGAUU TTATAAAGCAATGAGACTGATT 1425
236 hsa-mir-34a UGGCAGUGUCUUAGCUGGUUGU TGGCAGTGTCTTAGCTGGTTGT 1426
239 hsa-mir-361- UUAUCAGAAUCUCCAGGGGUAC TTATCAGAATCTCCAGGGGTAC 1427
5p 241 hsa-mir-363 AAUUGCACGGUAUCCAUCUGUA AATTGCACGGTATCCATCTGTA
1428 242 hsa-mir-365-2 UAAUGCCCCUAAAAAUCCUUAU
TAATGCCCCTAAAAATCCTTAT 1429 246 hsa-mir-374a UUAUAAUACAACCUGAUAAGUG
TTATAATACAACCTGATAAGTG 1430 250 hsa-mir-378 ACUGGACUUGGAGUCAGAAGG
ACTGGACTTGGAGTCAGAAGG 1431 259 hsa-mir-423 UGAGGGGCAGAGAGCGAGACUUU
TGAGGGGCAGAGAGCGAGACTTT 1432 265 hsa-mir-449a
UGGCAGUGUAUUGUUAGCUGGU TGGCAGTGTATTGTTAGCTGGT 1433 287 hsa-mir-503
UAGCAGCGGGAACAGUUCUGCAG TAGCAGCGGGAACAGTTCTGCAG 1434 296
hsa-mir-532 CAUGCCUUGAGUGUAGGACCGU CATGCCTTGAGTGTAGGACCGT 1435 316
hsa-mir-576- AAGAUGUGGAAAAAUUGGAAUC AAGATGTGGAAAAATTGGAATC 1436 3p
327 hsa-mir-625 AGGGGGAAAGUUCUAUAGUCC AGGGGGAAAGTTCTATAGTCC 1437
338 hsa-mir-7-1 UGGAAGACUAGUGAUUUUGUUGU TGGAAGACTAGTGATTTTGTTGT
1438 339 hsa-mir-7-2 UGGAAGACUAGUGAUUUUGUUGU
TGGAAGACTAGTGATTTTGTTGT 1439 340 hsa-mir-7-3
UGGAAGACUAGUGAUUUUGUUGU TGGAAGACTAGTGATTTTGTTGT 1440 345
hsa-mir-874 CUGCCCUGGCCCGAGGGACCGA CTGCCCTGGCCCGAGGGACCGA 1441 351
hsa-mir-9-1 UCUUUGGUUAUCUAGCUGUAUGA TCTTTGGTTATCTAGCTGTATGA 1442
352 hsa-mir-9-2 UCUUUGGUUAUCUAGCUGUAUGA TCTTTGGTTATCTAGCTGTATGA
1443 353 hsa-mir-92a-1 UAUUGCACUUGUCCCGGCCUGU
TATTGCACTTGTCCCGGCCTGT 1444 354 hsa-mir-92a-2
UAUUGCACUUGUCCCGGCCUGU TATTGCACTTGTCCCGGCCTGT 1445 356 hsa-mir-93
CAAAGUGCUGUUCGUGCAGGUAG CAAAGTGCTGTTCGTGCAGGTAG 1446 357
hsa-mir-9-3 UCUUUGGUUAUCUAGCUGUAUGA TCTTTGGTTATCTAGCTGTATGA 1447
364 hsa-mir-99a AACCCGUAGAUCCGAUCUUGUG AACCCGTAGATCCGATCTTGTG 1448
387 hsa-mir-570 AAAGGUAAUUGCAGUUUUUCCC AAAGGTAATTGCAGTTTTTCCCA
1449
TABLE-US-00019 TABLE 34 Primers for Reverse Transcription of a
subset of novel miRNAs identified by deep sequencing analysis. SEQ
Temporary Primer for Reverse SEQ ID Assigned Mature Sequence
Transcription ID NO. miRNA ID Captured of miRNA NO. 773 hsa-miR-
ACUGGACUUGGAGUCAGGA ACTGGACTTGGAGTCAGGA 1450 378c 774 hsa-mir-449c
AGGCAGUGUAUUGCUAGCGGCU AGGCAGTGTATTGCTAGCGGCTGT 1451 775
hsa-mir-500- UGCACCCAGGCAAGGAUUCUGC TGCACCCAGGCAAGGATTCTGC 1452 2
784 hsa-mir-2224 UGAGGGAGGAGACUGCA TGAGGGAGGAGACTGCA 1453 785
hsa-mir-2225 ACUGGACUUGGAGCCAGAAG ACTGGACTTGGAGCCAGAAG 1454 786
hsa-mir-2226 GUCACUGAUGUCUGUAGCUGAG GTCACTGATGTCTGTAGCTGAGACGG 1455
787 hsa-mir-2227 GAUGAGGAUGGAUAGCAAGGAA GATGAGGATGGATAGCAAGGAAG
1456 789 hsa-mir-2229 AAAAGCAUCAGGAAGUACCCA AAAAGCATCAGGAAGTACCCA
1457 796 hsa-mir-2235 GUCAAAUGAAGGGCUGAUCACG
GTCAAATGAAGGGCTGATCACGAAATA 1458 798 hsa-mir-2237
AGAGUUAACUCAAAAUGGACUA AGAGTTAACTCAAAATGGACTA 1459 799 hsa-mir-2238
UGUUGGGAUUCAGCAGGACCAU TGTTGGGATTCAGCAGGACCATT 1460 800
hsa-mir-2239 UAAAUAGAGUAGGCAAAGGACA TAAATAGAGTAGGCAAAGGACA 1461 805
hsa-mir-2244 AAGAGGAAGAAAUGGCUGGUUC AAGAGGAAGAAATGGCTGGTTCTCAG 1462
809 hsa-mir-2248 UAGUGGAUGAUGCACUCUGUGC TAGTGGATGATGCACTCTGTGC 1463
814 hsa-mir-2253 AAAGACUCUGCAAGAUGCCU AAAGACTCTGCAAGATGCCT 1464 816
hsa-mir-2255 AGGAGAAGUAAAGUAGAA AGGAGAAGTAAAGTAGAA 1465 817
hsa-mir-2256 AUGGCCAGAGCUCACACAGAGG ATGGCCAGAGCTCACACAGAGG 1466 819
hsa-mir-2258 AUCAGGGCUUGUGGAAUGGGAA ATCAGGGCTTGTGGAATGGGAAG 1467
820 hsa-mir-2259 AUGGCCAGAGCUCACACAGAGG ATGGCCAGAGCTCACACAGAGG 1468
821 hsa-mir-2260 UGAGGAUAUGGCAGGGAAGGGG TGAGGATATGGCAGGGAAGGGGA
1469 825 hsa-mir-2264 UGGGCUCAGGGUACAAAGGUU TGGGCTCAGGGTACAAAGGTTC
1470 828 hsa-mir- GCUGCACCGGAGACUGGGUAA GCTGCACCGGAGACTGGGTAA 1471
2266-1 829 hsa-mir- GCUGCACCGGAGACUGGGUAA GCTGCACCGGAGACTGGGTAA
1472 2266-2 836 hsa-mir-2272 UGUCGUGGGGCUUGCUGGCUUG
TGTCGTGGGGCTTGCTGGCTTG 1473 838 hsa-mir-2274 ACUGGACUUGGAGGCAGAA
ACTGGACTTGGAGGCAGAA 1474 841 hsa-mir-2277 UUGGAGGCGUGGGUUUU
TTGGAGGCGTGGGTTTT 1475 844 hsa-mir-2280 CUGACUGAAUAGGUAGGGUCAU
CTGACTGAATAGGTAGGGTCAT 1476 846 hsa-mir-2282 AGAUUGUUUCUUUUGCCGUGCA
AGATTGTTTCTTTTGCCGTGCA 1477 847 hsa-mir- CACGGCAAAAGAAACAAUCCA
CACGGCAAAAGAAACAATCCA 1478 2282* 848 hsa-mir-2283
CAGGGCUGGCAGUGACAUGGGU CAGGGCTGGCAGTGACATGGGT 1479 849 hsa-mir-2284
GGUGGGGGCUGUUGUUU GGTGGGGGCTGTTGTTT 1480 850 hsa-mir-2285
UGGGGAGGUGUGGAGUCAGCAU TGGGGAGGTGTGGAGTCAGCATG 1481 852
hsa-mir-2287 GGCUCCUUGGUCUAGGGGUA GGCTCCTTGGTCTAGGGGTA 1482 856
hsa-mir-2290 UGGGGAUUUGGAGAAGUGGUGA TGGGGATTTGGAGAAGTGGTGA 1483 857
hsa-mir-2291 AAAAGUGAUUGCAGUGUUUG AAAAGTGATTGCAGTGTTTGCC 1484 860
hsa-mir-2294 UAGGAGCUCAACAGAUGCCUGU TAGGAGCTCAACAGATGCCTGT 1485 861
hsa-mir-2295 AGCUUUUGGGAAUUCAGGUAG AGCTTTTGGGAATTCAGGTAG 1486 864
hsa-mir-2298 CAAAAGUGAUCGUGGUUUUUG CAAAAGTGATCGTGGTTTTTG 1487 865
hsa-mir-2299 AGGGUGUGUGUGUUUUU AGGGTGTGTGTGTTTTT 1488 871
hsa-mir-2305 ACUGACAGGAGAGCAUUUUGA ACTGACAGGAGAGCATTTTGA 1489 874
hsa-mir-2308 AUAGUGGUUGUGAAUUUACCUU ATAGTGGTTGTGAATTTACCTTC 1490
876 hsa-mir-2310 CUACCCCAGGAUGCCAGCAUAG CTACCCCAGGATGCCAGCATAGTT
1491 877 hsa-mir-2311 ACUGGACUUGGUGUCAGAUGG ACTGGACTTGGTGTCAGATGG
1492 880 hsa-mir-2314 UAGUGGAUGAUGGAGACUCGGU TAGTGGATGATGGAGACTCGGT
1493 888 hsa-mir-2322 AAGGUUUGGAUAGAUGCAAUA AAGGTTTGGATAGATGCAATA
1494 889 hsa-mir-2323 AAAGGUAAUUGCAGUUUUUCCC
AAAGGTAATTGCAGTTTTTCCCA 1495 890 hsa-mir-2324
AGGGGACCAAAGAGAUAUAUAG AGGGGACCAAAGAGATATATAG 1496 896 hsa-mir-2328
GGCGACAAAACGAGACCCUGU GGCGACAAAACGAGACCCTGTC 1497 897 hsa-mir-2329
GGGUGCGGGCCGGCGGGG GGGTGCGGGCCGGCGGGGT 1498 899 hsa-mir-2331
CAUGCUAGGAUAGAAAGAAUGG CATGCTAGGATAGAAAGAATGG 1499 901 hsa-mir-2333
GCAAAGUGAUGAGUAAUACUGG GCAAAGTGATGAGTAATACTGG 1500 915 hsa-mir-2346
CCCUGGGGUUCUGAGGACAUG CCCTGGGGTTCTGAGGACATG 1501 917 hsa-mir-2348
CAGGAAGGAUUUAGGGACAGGC CAGGAAGGATTTAGGGACAGGC 1502 919 hsa-mir-2350
AUUAAGGACAUUUGUGAUUGAU ATTAAGGACATTTGTGATTGAT 1503 920 hsa-mir-
AAAAGGCAUAAAACCAAGACA AAAAGGCATAAAACCAAGACA 1504 2351-1 921
hsa-mir- AAAAGGCAUAAAACCAAGACA AAAAGGCATAAAACCAAGACA 1505 2351-2
924 hsa-mir- UAAAAACUGCAAUUACUUUC TAAAAACTGCAATTACTTTC 1506 2354-1
926 hsa-mir- UGUGAUAUCAUGGUUCCUGGGA TGTGATATCATGGTTCCTGGGA 1507
2355-1 929 hsa-mir- UGUGAUAUCAUGGUUCCUGGGA TGTGATATCATGGTTCCTGGGA
1508 2355-2 931 hsa-mir- UGUGAUAUCAUGGUUCCUGGGA
TGTGATATCATGGTTCCTGGGA 1509 2355-3 932 hsa-mir-
UGUGAUAUCGUGCUUCCUGGGA TGTGATATCGTGCTTCCTGGGA 1510 2355b 936
hsa-mir-2358 AAAAGUAACUGCGGUUUUUGA AAAAGTAACTGCGGTTTTTGA 1511 938
hsa-mir-2360 GGAGUGGGCUGGUGGUU GGAGTGGGCTGGTGGTT 1512 940 hsa-mir-
AAGGGCUUCCUCUCUGCAGGAC AAGGGCTTCCTCTCTGCAGGAC 1513 2362-1 941
hsa-mir- AAGGGCUUCCUCUCUGCAGGAC AAGGGCTTCCTCTCTGCAGGAC 1514 2362-2
949 hsa-mir-2370 AGAGCUGGCUGAAGGGCAG AGAGCTGGCTGAAGGGCAG 1515 953
hsa-mir-2374 UGUGACUUUAAGGGAAAUGGCG TGTGACTTTAAGGGAAATGGCG 1516 954
hsa-mir-2375 UCUCAGGAGUAAAGACAGAGUU TCTCAGGAGTAAAGACAGAGTT 1517 955
hsa-mir-2376 AGGUGGAUGCAAUGUGACCUCA AGGTGGATGCAATGTGACCTCA 1518 977
hsa-mir-2397 AGGCUGGGCUGGGACGGA AGGCTGGGCTGGGACGGA 1519 979
hsa-mir-2399 UAGGAUGGGGGUGAGAGGUG TAGGATGGGGGTGAGAGGTG 1520 988
hsa-mir-2406 UGAGGGAGUAGGAUGUAUGGUU TGAGGGAGTAGGATGTATGGTT 1521 991
hsa-mir-2409 AGACUGACGGCUGGAGGCCCAU AGACTGACGGCTGGAGGCCCAT 1522 994
hsa-mir-2412 UAGUGAGUUAGAGAUGCAGAGC TAGTGAGTTAGAGATGCAGAGC 1523 996
hsa-mir-2414 GGGAGAAGGGUCGGGGC GGGAGAAGGGTCGGGGC 1524 1002
hsa-mir-2416 CUCGUGGGCUCUGGCCACGGC CTCGTGGGCTCTGGCCACGGCC 1525 1003
hsa-mir- AGAAGGGGUGAAAUUUAAACGU AGAAGGGGTGAAATTTAAACGT 1526 2417-1
1004 hsa-mir- AGAAGGGGUGAAAUUUAAACGU AGAAGGGGTGAAATTTAAACGT 1527
2417-2 1005 hsa-mir- AGAAGGGGUGAAAUUUAAACGU AGAAGGGGTGAAATTTAAACGT
1528 2417-3 1007 hsa-mir-2419 GCUCAGGGAUGAUAACUGUGCUGAGA
GCTCAGGGATGATAACTGTGCTGAGA 1529 1011 hsa-mir-2423
CUGGACUGAGCCAUGCUACUGG CTGGACTGAGCCATGCTACTGG 1530 1017
hsa-mir-2428 AUAGCAGCAUGAACCUGUCUCA ATAGCAGCATGAACCTGTCTCA 1531
1018 hsa-mir- UGAGACAGGCUUAUGCUGCUAU TGAGACAGGCTTATGCTGCTAT 1532
2428* 1022 hsa-mir-2432 UGGUCUGCAAAGAGAUGACUGU
TGGTCTGCAAAGAGATGACTGTG 1533 1025 hsa-mir-2435
UUGGAGGGUGUGGAAGACAUC TTGGAGGGTGTGGAAGACATC 1534 1027 hsa-mir-2437
AUGGAGAAGGCUUCUGA ATGGAGAAGGCTTCTGA 1535 1028 hsa-mir-2438
AAAAGCUGGGUUGAGAA AAAAGCTGGGTTGAGAAG 1536 1029 hsa-mir-2439
UGGAAGGUAGACGGCCAGAGAG TGGAAGGTAGACGGCCAGAGAG 1537 1030
hsa-mir-2440 UCUGGGAGGUUGUAGCAGUGGA TCTGGGAGGTTGTAGCAGTGGA 1538
1036 hsa-mir-2445 GGAGGAACCUUGGAGCUUCGGC GGAGGAACCTTGGAGCTTCGGCA
1539 1038 hsa-mir-2447 ACUGGACUAGGAGUCAGAAGG ACTGGACTAGGAGTCAGAAGG
1540 1041 hsa-mir-2450 CAAAAACUGCAGUUACUUUUGU
CAAAAACTGCAGTTACTTTTGT 1541 1046 hsa-mir-2455 AAAAGGCAUUGUGGUUUUUG
AAAAGGCATTGTGGTTTTTG 1542
TABLE-US-00020 TABLE 35 miRNAs that differentiate ABC DLBCL vs. GCB
DLBCL FoldChange pVal ABC vs. ABC vs. MicroRNA GCB GCB
UGGUCUGCAAAGAGAUGACUGUG -4.30138 0.001335 (SEQ ID NO. 1565)
CAAAAACUGCAGUUACUUUUGU -3.52085 0.022949 (SEQ ID NO. 1041)
UGGGGAUUUGGAGAAGUGGUGA -2.8374 0.000753 (SEQ ID NO. 856)
hsa-miR-129-3p -2.48708 7.39E-05 ACUGGACUUGGUGUCAGAUGG -1.3712
0.01173 (SEQ ID NO. 877) hsa-miR-196b -1.28463 0.032606 hsa-miR-9
-1.00737 0.003176 hsa-miR-28-5p -0.82319 0.00026 hsa-miR-365
-0.78573 0.032749 hsa-miR-185 -0.75345 0.044082 hsa-miR-199b-3p
-0.74898 0.015518 hsa-miR-152 -0.67388 0.001282 hsa-miR-23a
-0.65979 0.028732 hsa-miR-193a-5p -0.54233 0.031154 hsa-miR-27a
-0.49729 0.027207 hsa-miR-331-3p -0.47573 0.0128 hsa-miR-301a
-0.45261 0.014231 hsa-miR-128 -0.4224 0.024065
AGAUUGUUUCUUUUGCCGUGCA 0.557435 0.02141 (SEQ ID NO. 846)
hsa-miR-625 0.747299 0.01813 hsa-miR-155 0.781901 0.024175
hsa-miR-20b 0.986918 0.000746 GGCUCCUUGGUCUAGGGGUA 1.945372
0.002596 (SEQ ID NO. 852) UAGUGAGUUAGAGAUGCAGAGC 2.347695 0.009081
(SEQ ID NO. 994) CAGGAAGGAUUUAGGGACAGGC 2.519326 0.04483 (SEQ ID
NO.-917) *The 3'-end G of SEQ ID NO. 1565 is optionally omitted
(providing SEQ ID NO. 1022).
TABLE-US-00021 APPENDIX A Average expression of the genes depicted
in FIG. 6* Naive vs Germinal Germinal Germinal Center vs Center vs
Center Plasma Memory Naive GC GC PC GC Aver- Aver- aver- Aver-
Aver- Memory ID Gene Name age age age age age Average 1 VPS37B
10.60 4.58 4.58 9.71 2 SCN3A 9.50 3.98 3 NT5E 10.76 5.69 4 TBC1D9
12.03 7.03 7.03 11.19 7.03 12.88 5 MAP7 1.64 8.03 6 SPRY1 10.40
5.72 7 TNFSF12 8.11 3.54 3.54 7.55 8 SOCS3 9.00 4.48 4.48 9.39 9
EDG1 11.43 6.91 6.91 8.69 10 CTGF 7.62 3.30 3.30 9.65 11 FAM46A
10.65 6.46 6.46 8.29 12 MOBKL2B 11.63 7.45 13 DNMT3A 10.56 6.44
6.44 7.85 14 BHLHB2 10.75 6.64 6.64 10.96 15 LAMC1 9.85 5.80 5.80
9.67 16 CD69 13.27 9.40 9.40 12.27 17 PLEKHA1 10.95 7.20 18 PTGDR
7.20 3.46 19 TXNIP 14.89 11.20 11.20 13.39 11.20 15.10 20 SIDT1
11.52 7.86 7.86 9.38 21 LY6E 8.57 4.92 4.92 7.39 22 IGF1R 8.73 5.13
23 PRICKLE1 11.68 8.09 8.09 9.69 24 CSDA 12.12 8.59 8.59 10.10 25
KLF2 12.61 9.10 9.10 11.70 26 ELOVL2 7.32 3.86 27 CCND2 12.06 8.61
8.61 11.53 28 LASS6 10.29 6.86 6.86 9.42 6.86 9.48 29 DUSP6 11.93
8.53 8.53 10.19 30 CUGBP2 14.28 10.93 10.93 14.29 31 PTPRO 9.64
6.32 6.32 8.63 32 PHF16 10.33 7.04 33 NR3C2 8.85 5.58 34 CRTC3
12.56 9.32 9.32 11.11 35 ADAMTS6 7.68 4.45 36 ETV6 10.36 7.14 7.14
8.89 7.14 10.43 37 LRRC17 3.10 7.49 38 FAM46C 12.18 8.98 8.98 14.76
8.98 10.54 39 SATB2 7.20 4.02 4.02 7.60 40 RNF125 9.00 5.83 41
ST6GALNAC3 8.12 4.96 42 LARGE 9.05 5.89 5.89 8.73 43 ZNF276 9.39
6.25 44 KCNA3 4.83 9.82 45 BCL2 10.89 7.76 7.76 11.41 46 MTSS1
12.92 9.82 9.82 12.05 47 NR6A1 9.10 6.03 6.03 7.62 48 BHLHB3 11.11
8.05 8.05 13.26 8.05 13.26 49 MYO10 7.22 4.19 50 ITM2C 6.44 13.85
51 C18orf1 9.49 6.47 6.47 8.63 52 FXYD7 7.36 4.35 53 DUSP8 7.96
4.96 4.96 8.41 4.96 7.75 54 BTBD3 7.41 4.42 55 TMEPAI 11.15 8.20
8.20 11.03 56 ANTXR2 9.90 6.97 57 FOSB 10.13 7.21 7.21 11.05 58
TMCC3 8.23 5.31 5.31 7.09 5.31 8.05 59 ARL4C 11.31 8.41 8.41 10.32
60 ZMYND11 11.33 8.43 8.43 11.34 8.43 11.68 61 RHOBTB1 7.33 4.48 62
JUN 12.14 9.34 9.34 12.70 63 SKI 10.46 7.67 7.67 10.68 64 TMEM121
7.07 4.29 65 IL13RA1 8.99 6.21 66 KIF13B 6.23 9.01 67 BHLHB5 4.26
7.39 68 PGM2L1 10.35 7.65 69 C14orf4 12.11 9.47 9.47 11.01 9.47
11.65 70 PDE4B 12.38 9.74 9.74 11.60 71 PDE7B 8.64 6.03 72 BCL9L
9.37 6.77 6.77 8.61 73 PCDH9 10.32 7.73 74 ARHGAP5 10.44 7.86 7.86
9.11 7.86 9.85 75 KIAA0802 9.18 6.61 6.61 7.91 6.61 7.89 76 ITGB4
7.48 4.91 77 NOTCH2NL 7.17 8.95 78 CDC42BPA 7.32 4.77 79 SPG20 9.25
6.73 80 KLF4 10.04 7.57 7.57 9.09 81 AHNAK 9.34 6.87 6.87 9.56 6.87
11.51 82 FCHSD1 9.15 6.69 83 KIAA1622 3.94 7.67 84 PLXNC1 10.92
8.48 85 TMEM150 8.39 5.96 86 ACVR2A 7.81 5.38 5.38 8.63 5.38 7.40
87 KALRN 5.61 7.64 88 ANK2 7.69 5.28 5.28 7.54 5.28 7.00 89
FLJ14213 7.61 5.21 5.21 7.97 90 CHPT1 10.92 8.52 91 TCF2 5.69 7.82
92 FGF5 5.41 7.52 93 SLC12A6 12.18 9.80 9.80 11.09 94 MGC17330
11.53 9.16 95 NR4A2 10.21 7.85 7.85 9.21 7.85 10.54 96 SLC39A10
11.06 8.70 97 LITAF 12.52 10.18 10.18 11.82 98 AKT3 9.32 6.99 6.99
9.25 99 PDCD4 12.89 10.57 10.57 13.33 100 STMN3 10.03 7.72 101
SIDT2 13.13 10.85 102 GRIA3 7.04 4.77 103 EML4 12.83 10.57 104
DIP2B 11.26 8.99 8.99 10.77 105 FBN1 4.89 8.17 106 FAM84B 9.21 6.96
107 EGR3 11.59 9.35 9.35 8.24 9.35 10.62 108 CTHRC1 4.25 11.94 109
RRAGD 7.69 5.46 110 MACF1 12.88 10.66 111 FOXF2 7.05 4.83 112 GAB1
8.01 5.81 5.81 10.91 113 ST18 4.42 7.40 114 ZFP36L2 13.78 11.59
11.59 13.43 11.59 14.05 115 GAB2 8.29 6.12 6.12 9.02 116 CHST11
11.17 8.99 8.99 10.28 117 CLOCK 9.85 7.68 7.68 9.91 7.68 9.62 118
PREX1 10.07 7.90 7.90 11.16 119 KLF11 8.08 5.94 5.94 8.39 120
PTGER4 11.66 9.53 121 KLF9 9.47 7.34 7.34 9.27 122 FLJ37078 7.55
5.42 5.42 7.49 123 ODZ2 7.20 5.08 5.08 7.25 5.08 7.22 124 SESN3
11.24 9.11 9.11 7.60 125 PDK4 7.22 5.09 126 CNTNAP3 7.35 5.23 5.23
7.10 127 DUSP1 13.34 11.22 128 MARCKS 11.19 9.07 9.07 11.05 9.07
12.21 129 SPRY4 7.02 4.91 130 LMO1 4.75 7.16 131 MTUS1 7.77 5.66
5.66 8.16 132 ADCY9 8.01 5.91 5.91 7.74 133 SLC17A6 3.80 7.06 134
NOX4 7.45 5.37 5.37 7.22 135 UTRN 9.46 10.97 136 ZBTB10 8.50 6.45
137 SLC26A7 8.37 6.32 6.32 7.89 138 PNRC1 13.14 11.09 139 LLGL2
6.83 9.29 140 CHST1 7.15 5.12 141 CREB3L2 10.53 8.51 8.51 13.30 142
DDIT4 12.69 10.68 143 C20orf108 8.82 6.80 6.80 10.19 144 CDH1 3.85
10.26 145 TFAP4 7.28 5.29 146 SLC38A2 13.57 11.58 11.58 13.74 11.58
13.17 147 SESN1 9.84 7.87 7.87 9.13 148 YPEL2 10.18 8.20 8.20 10.04
149 GRASP 8.47 6.50 150 TSC22D3 13.22 11.26 11.26 12.67 151 ATP11A
9.70 7.75 7.75 9.50 152 L3MBTL3 10.61 8.66 8.66 10.06 153 SORT1
7.61 5.67 5.67 8.11 154 CAV1 4.80 9.11 155 RXRA 9.07 7.15 7.15 8.81
156 CRELD1 8.40 6.48 6.48 8.26 157 RBMS1 12.40 10.49 10.49 8.80 158
LYST 10.90 8.99 159 PIP5K1B 9.61 7.70 7.70 9.97 160 JUNB 11.62 9.72
161 MBOAT1 4.80 7.27 162 IRF4 11.16 9.28 9.28 13.66 163 LIFR 4.62
7.08 164 MORC3 11.25 9.37 165 MBP 11.91 10.05 10.05 8.61 166 SRC
4.98 7.98 167 ALS2CR13 11.79 9.93 9.93 8.89 9.93 11.47 168 MYH1
4.40 7.37 169 DUSP3 9.36 7.50 7.50 9.16 170 HLX1 7.14 5.28 5.28
7.65 171 CDKN1A 9.09 7.23 7.23 8.97 7.23 8.43 172 SOCS5 10.00 8.15
173 PPP1R9A 5.14 7.35 174 TGFBR2 12.81 10.99 10.99 12.63 175 LRRC16
8.47 6.66 176 ZNF629 8.17 6.35 6.35 8.58 177 RPS6KA5 11.75 9.95
9.95 11.48 178 SATB1 12.79 10.99 10.99 9.06 179 SEMA4C 7.38 5.59
5.59 8.49 5.59 7.14 180 ULK1 7.79 5.99 5.99 8.72 5.99 8.06 181 STX3
8.88 7.09 7.09 8.91 182 BAMBI 4.81 7.26 183 MAP3K5 10.63 8.84 8.84
7.66 8.84 10.71 184 KIAA1147 11.70 9.91 9.91 11.53 185 SBK1 5.20
7.04 186 RYR3 7.80 6.02 6.02 8.27 187 ZNF238 13.05 11.27 11.27
10.08 11.27 12.54 188 IL12A 5.65 7.26 189 SLC2A3 12.00 10.23 10.23
11.89 190 GFPT2 3.39 7.18 191 G0S2 7.72 5.96 192 LYRM5 10.29 8.53
8.53 10.93 193 SSH2 11.67 9.91 9.91 11.85 194 NRP1 7.44 5.69 5.69
7.22 195 LMLN 8.92 7.19 196 UCP3 7.71 5.99 197 TMEM166 7.31 5.60
198 CACNA1I 7.46 5.76 5.76 7.06 199 PHOSPHO1 3.55 7.10 200 CRIM1
9.79 8.11 8.11 9.32 201 GATA6 5.41 7.59 202 SACS 10.06 8.39 8.39
10.54 203 CDKN1B 12.91 11.24 11.24 14.41 204 CACNA2D2 5.25 7.57
5.25 7.93 205 MKRN3 3.65 7.10 206 MTFR1 10.40 8.74 8.74 7.34 8.74
10.15 207 GALNT3 10.19 8.54 208 RPS6KA3 12.07 10.42 10.42 11.48 209
DTNA 5.47 7.86 210 MAGI2 7.04 5.40 5.40 7.42 211 FOXJ2 10.01 8.38
8.38 9.97 8.38 9.85 212 KIAA0513 5.29 8.44 5.29 7.36 213 NDRG1 7.75
10.02 214 AKAP7 10.16 8.54 215 CD72 13.74 12.12 216 IGFBP5 7.30
5.69 5.69 7.59 217 REPS2 7.51 5.92 5.92 7.60 218 PRDM12 7.17 5.57
219 ZNF3 8.86 7.27 7.27 8.75 220 TLL2 4.88 7.01 221 PCNX 9.77 8.19
222 ARHGAP24 11.31 9.73 9.73 8.65 9.73 11.32 223 THRAP2 11.31 9.73
224 RNF11 9.72 8.15 8.15 11.08 225 HOXC8 7.05 5.48 226 SCML2 10.33
8.76 227 BMPR2 9.70 8.14 8.14 10.06 228 STAC 5.27 7.20 229 C10orf54
9.12 7.56 7.56 10.16 230 FBXL17 9.51 7.95 7.95 9.36 231 CBX7 10.87
9.32 9.32 10.96 232 UBE2W 9.80 8.25 8.25 9.76 8.25 9.38 233
ProSAPiP1 4.12 7.54 234 UBL3 10.73 9.18 9.18 11.27 9.18 11.03 235
TTYH3 7.35 5.81 5.81 8.16 236 PUNC 5.39 8.85 5.39 7.30 237 GDF11
7.01 5.47 5.47 7.70 238 LMX1A 5.25 7.43 239 TIMP2 7.51 5.97 5.97
7.69 5.97 7.40
240 NHLH2 3.93 7.35 241 CLCF1 10.50 8.98 242 ITGB3 7.66 6.14 243
TMEM132E 3.93 7.20 244 KCTD17 7.45 5.94 5.94 7.33 245 ChGn 7.75
6.24 6.24 8.36 246 DIP2C 9.25 7.75 247 DKFZp667G2110 8.79 7.28 7.28
9.25 248 CSNK1G3 10.84 9.34 9.34 10.70 249 NRIP1 11.79 10.30 250
SMAD3 10.72 9.23 9.23 7.74 9.23 10.54 251 SHOX2 7.05 5.57 252
LDLRAP1 6.80 9.84 6.80 8.59 253 SUPT3H 7.77 6.30 6.30 8.05 254
KCNN2 7.18 5.70 255 DLL1 7.37 5.90 256 CEND1 7.72 6.26 257 NOTCH1
10.90 9.45 258 TLE1 7.74 9.63 259 FCHO2 8.75 7.30 260 JPH4 7.12
5.67 5.67 7.60 261 KCNMB2 7.16 5.72 262 KHDRBS2 7.17 5.73 263
LEFTY1 4.38 7.21 264 ST3GAL5 10.59 9.15 265 LGR4 5.30 7.29 266
FNDC8 4.25 8.01 267 NPTXR 5.87 7.65 268 PRICKLE2 5.47 7.47 269
GRIA4 5.19 7.04 270 RHOB 8.66 7.23 7.23 9.56 271 ZADH2 8.65 7.22
272 ZBTB41 9.73 8.31 8.31 9.42 273 GPRASP2 6.00 7.35 274 SYS1 10.79
9.36 275 RUNX1T1 7.37 5.95 276 DLX2 4.66 7.04 277 SLC30A7 11.58
10.17 278 PER1 8.82 7.40 279 NT5C3 12.67 11.25 280 PDE3A 11.62
10.21 281 OCRL 8.07 6.66 6.66 8.04 282 PSD3 7.99 6.60 283 LPHN1
7.80 6.41 284 TNRC6B 11.87 10.48 10.48 11.77 285 SNN 12.03 10.64
10.64 8.91 10.64 12.10 286 HERPUD2 11.56 10.18 287 UBQLNL 7.45 6.07
288 HES7 7.37 5.99 289 GALNT2 10.08 8.69 8.69 10.63 290 CAMKK1 8.45
7.07 291 ELN 5.44 7.18 292 ICK 8.09 6.72 6.72 8.01 293 POU4F2 7.09
5.72 5.72 7.21 294 GAS2 7.13 5.76 5.76 7.81 295 ARHGEF3 10.11 8.75
8.75 11.04 296 ZBTB4 11.45 10.09 10.09 11.78 297 CHD7 12.14 10.78
10.78 12.08 298 TMEM45B 5.97 7.61 299 CLDN11 5.74 7.22 300 PTPN1
10.85 9.51 301 PHF20 12.00 10.67 302 VAV3 10.44 7.84 303 CARKL 8.38
7.06 7.06 8.94 304 TRIM36 5.43 7.77 305 CTLA4 6.57 7.95 306 POLK
9.69 8.38 8.38 10.55 307 WSB1 13.22 11.91 11.91 13.02 308 ALS2CR2
9.87 8.57 8.57 10.14 309 PLK2 4.94 7.13 310 SRPK2 10.83 9.53 9.53
11.13 311 ARRDC2 10.86 9.55 312 IER5 13.10 11.80 313 EPN1 8.95 7.66
7.66 9.07 314 SLC20A2 9.45 8.16 315 DLG4 4.23 7.22 316 TMTC2 6.16
7.92 317 ETV1 8.19 6.90 318 JAZF1 11.93 10.65 10.65 7.49 319 VAV2
10.22 8.94 320 C15orf27 7.19 5.91 321 FYCO1 9.75 8.47 322 KIAA0789
7.27 6.00 323 OBFC2A 9.94 8.66 8.66 10.94 324 MCF2 7.19 5.92 325
KIAA2018 11.77 10.50 10.50 9.50 326 MTMR10 10.75 9.48 327 FAM63B
6.03 7.83 6.03 7.95 328 SNF1LK 10.20 8.93 8.93 10.18 329 ZNF385
8.89 7.63 330 SESTD1 11.05 9.78 331 SLC31A2 5.41 9.13 332 PCMTD1
12.03 10.76 10.76 12.44 333 NBEA 8.06 6.81 6.81 8.02 6.81 8.35 334
ZNF295 9.24 10.61 335 SIPA1L3 10.81 9.57 336 CC2D1B 9.11 7.87 337
PRKAG2 7.12 8.42 338 PKD1 9.04 7.81 339 CNTNAP2 7.54 6.32 340
FNBP1L 7.90 6.67 341 HEXIM1 10.27 9.05 9.05 10.24 342 C19orf2 12.29
11.07 343 MYLIP 11.62 10.41 10.41 11.81 344 SLC11A2 9.27 8.06 345
CLU 9.92 8.71 346 GCN5L2 10.18 8.97 347 DKFZP564J0863 10.85 9.65
9.65 11.11 348 CNNM2 7.85 6.64 6.64 8.07 349 CDC42SE1 12.07 10.87
10.87 12.18 350 HOXB9 6.20 8.41 351 NFATC3 11.39 10.19 352 UNC84B
11.12 9.93 9.93 8.59 353 DUSP5 6.23 9.44 354 ING1 11.35 10.17 355
ITM2B 11.23 13.62 356 FAM53B 11.67 10.49 357 ZFP36L1 14.07 12.89
12.89 10.09 358 NIPA1 9.75 8.57 8.57 10.07 359 GALNT1 10.93 9.76
9.76 11.12 360 MYPN 4.25 7.32 361 ITPK1 10.03 8.86 362 TTYH2 7.90
6.73 363 DOCK10 10.80 9.63 9.63 11.53 364 C14orf28 9.18 8.01 8.01
9.58 8.01 9.50 365 RP11-130N24.1 4.76 7.62 366 FGF12 5.82 7.33 367
ATP2B2 7.93 6.76 368 PPP1R9B 9.77 8.60 369 PPP3CA 13.50 12.33 12.33
13.86 370 TFAP2A 5.54 8.26 371 CYLD 10.91 9.75 372 PHF1 11.85 10.69
10.69 12.26 373 NEBL 7.98 6.82 6.82 8.08 374 ACIN1 12.04 10.89
10.89 11.99 375 SPTBN1 12.28 11.14 11.14 12.53 11.14 12.42 376
VAMP4 10.81 9.67 9.67 11.18 377 DNAJB9 10.80 9.66 9.66 15.21 9.66
11.03 378 ZDHHC2 11.55 10.42 379 TRIO 10.57 9.43 380 TMEM25 6.24
8.57 381 TAF9B 10.82 9.69 382 ARID3A 8.03 6.90 6.90 9.81 383
KIAA0182 10.68 9.55 384 RPS6KA2 8.61 7.49 385 C3orf58 11.47 10.34
386 CAST 11.94 10.82 387 SH3PXD2A 8.46 7.34 388 RAB6B 5.93 7.36 389
RNF141 11.47 10.35 390 SP4 11.26 10.14 391 ARMCX2 9.44 8.32 8.32
9.54 392 ZNF398 10.39 9.28 393 PBX3 9.69 8.58 8.58 7.00 394 FOS
12.35 11.25 11.25 13.34 395 FHOD3 6.40 7.94 396 C20orf59 10.11 9.01
9.01 10.92 397 FAM117A 11.67 10.57 10.57 12.06 10.57 11.64 398
ATP8B2 8.58 7.49 7.49 10.87 7.49 8.88 399 UXS1 9.53 8.43 400
GOLGA8A 12.84 11.74 11.74 13.14 11.74 13.05 401 SUFU 6.03 7.84 402
NAGPA 9.32 8.23 403 MLLT6 11.70 10.61 404 CPEB4 9.46 8.36 8.36
12.21 405 TMEM50B 10.67 9.58 9.58 11.41 9.58 10.63 406 AMPD3 11.65
10.56 10.56 8.76 407 SIX5 5.19 7.53 408 MMP16 5.66 7.47 409 LBH
11.87 10.79 10.79 8.83 410 POM121 9.60 11.26 411 ATP8B1 11.93 10.86
10.86 8.88 412 ARHGAP29 3.75 7.60 413 ZNF395 11.78 10.76 414 CRY2
6.50 8.01 415 BIN1 10.68 9.67 416 PSAP 11.24 13.41 11.24 12.71 417
ISL1 5.91 7.60 418 DNAJB5 8.04 6.97 419 KIAA0284 6.42 8.79 420
RBM35B 11.44 10.38 421 ADCY7 11.53 10.47 422 ARRDC3 9.46 10.68 423
MICAL1 11.72 10.67 424 MTHFR 10.31 9.26 425 HOOK3 8.57 7.53 7.53
6.52 426 EFNB3 5.16 7.79 427 ARHGAP12 9.86 8.82 428 LMBR1L 9.33
8.29 429 FGF7 5.88 7.86 430 USP2 5.50 7.52 431 SMARCA2 11.14 10.12
432 ELL2 8.23 13.60 433 C1orf26 7.06 10.01 434 CXCR4 14.61 13.45
435 HDGFRP3 5.58 7.39 436 BNC2 6.18 7.72 437 YPEL3 10.16 11.81
10.16 11.47 438 PPFIA3 4.58 8.06 439 DLGAP4 9.34 10.68 440 ZBTB7A
9.84 11.10 441 D4S234E 5.90 7.38 442 FNDC3A 9.41 13.60 443 FOXC1
5.84 7.39 444 KIF26B 5.55 7.30 445 NAV2 6.26 7.90 446 DDX3Y 8.07
10.38 447 FGD1 7.31 6.06 448 HTR4 7.26 9.01 7.26 8.40 449 C22orf31
5.67 8.10 450 RNF44 11.48 10.14 451 GALNAC4S-6ST 10.43 12.71 452
CCNT2 10.15 11.23 453 CHIC1 8.91 10.02 454 NNAT 5.97 8.19 5.97 7.84
455 CTDP1 6.81 8.26 456 BTG1 14.95 13.94 457 CMTM4 5.27 7.81 458
GOLGA8B 11.37 12.63 459 HBP1 10.07 12.13 460 GDPD1 6.35 7.61 461
CDYL 9.31 10.61 9.31 10.50 462 ZNF217 10.77 9.73 463 KIF5A 6.13
7.75 464 C1QL1 5.80 8.11 465 SOLH 7.88 9.03 466 ZC3H6 9.81 10.96
467 LATS2 7.61 10.19 468 COL18A1 6.46 7.85 469 C11orf24 9.89 11.07
470 SCUBE3 6.14 7.88 471 SEMA4G 5.57 7.81 472 FOXP1 12.31 10.84 473
TRIB2 10.62 9.61 474 LOC285382 5.35 7.20 475 RUNX2 7.91 10.22 476
LOC196463 4.16 7.52 477 LPGAT1 10.57 7.99 478 RASSF2 12.52 10.93
479 IRF1 9.86 11.60 480 RAB40B 7.31 10.89 481 CTDSPL 6.37 7.83 482
CLCN4 7.44 6.39 483 CACNB1 6.41 8.20 484 SYNGR1 8.45 10.40 485
ST8SLA4 10.67 11.81 486 PLD3 7.63 10.59 487 FOXO3A 10.17 12.41 488
TSPAN33 10.49 9.38 489 HIST1H4F 6.20 8.08 490 LAPTM4A 12.05
13.06
491 PRKCB1 12.14 10.50 492 PROX1 6.38 7.89 493 CDK5R1 10.12 7.78
494 MAP3K9 7.96 9.13 495 GPX3 5.92 7.61 496 GNS 8.79 11.06 497
ARL15 8.27 6.51 498 OXR1 9.21 11.17 9.21 10.25 499 AAK1 7.38 9.54
7.38 8.47 500 SH3PX3 6.72 8.58 501 MS4A7 10.55 8.35 502 FLJ20273
5.96 11.23 5.96 8.65 503 ISCU 12.01 13.55 504 ITGA2 5.70 7.60 505
ME1 5.87 7.89 506 LRP1 4.92 7.04 507 ZNF652 9.66 10.81 508 TRAK1
11.07 9.85 509 SLC8A1 6.27 7.83 510 C1orf119 10.76 11.98 511 KLF6
11.33 9.24 11.33 12.40 512 TRIM2 6.22 8.20 513 USP3 11.11 12.51 514
ARID5B 11.02 12.48 515 RASD1 6.15 9.32 516 ZCCHC2 10.63 8.93 517
LEFTY2 5.08 7.03 518 BACH1 9.97 11.76 519 IRAK1 11.48 12.80 520
RP11-217H1.1 10.76 13.04 521 HLCS 6.54 8.45 522 NAGK 10.94 12.14
523 CELSR2 6.26 7.89 524 PCBP4 7.75 9.08 525 FLJ25476 9.49 10.72
526 TPP1 12.10 11.06 527 ACVR1 7.95 9.31 528 EHD3 8.99 10.35 529
FAM80B 9.35 8.02 530 SPRYD3 8.19 9.51 531 PRDM4 9.81 10.83 532
C6orf134 7.92 5.60 533 HSP90B1 12.04 16.36 534 DYNC1I1 5.98 7.33
535 NFLX 7.43 8.67 536 DOCK4 5.96 7.97 537 ZNF287 7.09 8.47 538
XRN1 10.92 12.37 539 YES1 6.39 8.60 540 RBM35A 8.19 9.44 541 HOXB4
7.58 9.27 542 OTUD7B 7.24 8.57 543 CELSR3 6.83 8.39 544 RHOC 7.71
8.84 545 ZNF607 8.10 6.95 546 XYLT1 10.28 8.78 10.28 8.86 547
FAM89B 9.16 10.75 548 OSBPL8 12.99 11.66 549 SRCRB4D 6.82 8.67 550
RASL12 5.05 8.07 551 NAG8 8.39 9.78 552 MAN1A2 10.83 12.15 553
PPARA 8.59 9.65 554 CLDN12 6.66 8.39 555 ID4 5.93 7.79 556 HECTD2
7.98 6.80 557 EFCAB4A 5.93 8.96 558 CREBL1 6.97 8.09 559 SARM1 6.98
8.29 560 MIDN 8.48 7.29 561 EMB 10.48 8.65 562 FOXK2 8.40 9.63 563
INADL 6.87 8.44 564 KCNAB1 6.26 9.76 565 MAP3K8 10.92 9.60 566
C8orf58 7.89 5.62 567 SLC4A7 9.82 8.52 9.82 10.90 568 RAB8B 12.46
10.92 569 C10orf118 8.93 10.20 570 CRTC2 9.37 7.94 571 KLC2 7.31
9.19 572 SRCAP 8.06 6.96 573 CNTFR 7.15 8.67 574 SPTBN2 4.75 7.11
575 SMAD7 8.69 9.93 576 NDFIP1 9.10 10.95 577 BCL11A 14.03 10.63
578 SFXN5 7.26 8.87 579 RIMBP2 5.93 8.33 580 FUT8 8.62 11.08 581
PSEN2 5.86 9.17 582 MAP6 5.22 7.37 583 FOXO1A 11.53 10.14 584 BTG2
11.81 13.82 585 C10orf56 6.93 9.30 586 MAPK1 12.06 10.36 587 ZBTB47
6.63 8.12 588 GOSR2 9.73 11.07 589 ZFP90 10.15 8.92 590 RALGPS1
8.02 9.38 591 DGKI 4.77 7.39 592 RGL2 11.08 9.55 593 PAK6 5.90 8.55
594 DMXL1 11.85 10.31 595 TMEM113 12.28 11.03 596 SNX24 6.75 8.53
597 HOXA3 5.99 8.27 598 SAMD4A 9.84 8.36 599 WDR45 9.88 12.43 600
TLOC1 12.91 14.52 601 LARP2 7.64 11.50 602 DTX2 7.60 6.20 603 ITGA9
7.53 6.32 604 FASTK 10.04 11.22 605 TAOK2 7.47 6.37 606 CRTAP 9.78
11.08 607 SERTAD2 11.91 10.82 608 TBP 10.14 9.09 609 IQGAP2 7.26
11.19 7.26 8.93 610 RASGEF1B 6.23 8.01 611 PIP5K2B 9.69 8.53 612
PRRC1 10.32 11.92 613 RHPN2 6.73 8.21 614 DYRK1B 5.92 7.61 615
ADCY2 5.98 7.97 616 C12orf34 5.61 7.09 617 GMPR 5.35 8.15 618 PAK2
10.86 9.66 619 KIAA1539 8.12 9.49 620 PLAGL1 10.03 6.68 621 LCORL
9.74 11.29 622 BCL9 7.21 9.16 623 CPEB2 9.09 10.23 624 TMEM59 12.10
14.75 625 TRIP10 7.17 9.36 626 CCPG1 8.38 11.86 627 NDE1 10.71 9.30
628 ZDHHC21 9.38 8.11 629 STOX2 5.87 7.66 630 RAB4B 10.63 8.66 631
LRRFIP1 13.25 11.99 632 OSBP 10.10 11.39 633 RAB6A 10.76 12.27 634
RHOV 4.88 7.19 635 SLC39A14 8.80 11.00 636 ADARB1 10.05 7.69 637
ESPN 6.85 8.19 638 SEC31A 11.64 13.61 639 PJA2 10.85 11.99 640
SYNGR3 9.01 7.56 641 LOC339745 11.94 10.54 642 NEDD9 11.23 12.48
643 COL23A1 5.41 7.02 644 PIP5K3 11.93 10.79 645 PTPRG 7.30 8.49
646 DAB2IP 5.61 8.30 647 MAPRE3 5.80 8.60 648 SQSTM1 10.05 11.45
649 RAB6C 10.88 12.04 650 FAM57A 7.58 6.18 651 YPEL5 12.41 13.78
652 TTN 9.61 7.03 653 HTRA3 5.62 8.02 654 CHST2 7.80 12.49 655
BAZ1A 11.43 10.06 656 LRFN5 7.38 6.21 657 MBNL1 14.10 12.62 658
MLL2 10.93 9.89 659 SF1 11.44 10.36 660 FRMPD1 4.80 7.06 661
PPP2R5B 7.60 9.12 662 RNF43 10.04 8.01 663 GAP43 5.07 7.49 664
NOMO1 11.11 14.31 665 VEGFA 7.79 9.36 666 C22orf5 8.75 11.38 667
AKAP11 11.79 10.64 668 INSIG2 9.46 11.32 669 PDIK1L 9.11 10.72 670
TMEM4 10.93 13.32 671 LMNA 7.80 9.75 672 TP53INP1 11.84 14.14 673
NAV1 6.96 8.31 674 SPTY2D1 9.25 10.77 675 CREBL2 9.95 11.10 676
MFAP3L 5.90 7.46 677 REXO2 10.96 13.71 678 SPTBN4 6.77 8.22 679
NOMO2 9.74 12.94 680 PTEN 12.78 11.40 681 FBXL16 6.86 9.56 682
GPM6A 7.87 6.15 7.87 6.67 683 PRX 6.76 8.91 684 HOXC11 6.40 7.88
685 KIAA0329 8.27 9.87 686 PAFAH1B1 11.68 10.33 687 C20orf174 9.58
7.27 688 MTPN 13.87 12.17 689 TMED5 12.25 14.41 690 FURIN 8.03 9.54
691 ELAVL3 4.66 8.01 692 SH2B3 10.31 8.87 693 LIN28B 5.99 7.58 694
KIAA1033 12.65 11.38 695 TMEM28 4.95 7.14 696 TBC1D15 10.30 11.52
697 GOLGA4 10.11 11.71 698 STX1A 5.63 7.82 699 SLC40A1 6.87 8.27
700 KIAA1815 9.18 7.93 701 HNT 5.53 7.09 702 PDE11A 5.72 7.82 703
TGFBR3 7.84 9.52 704 MTF1 9.92 8.86 705 MAN1A1 9.99 13.65 706 THRA
7.26 8.48 707 CBLB 11.46 9.08 708 SLC35F1 6.29 8.16 709 FOXP4 8.52
7.30 710 ELOVL5 13.83 12.78 711 NMUR1 4.13 7.31 712 ITPR1 12.06
9.69 713 CDCA7L 11.95 10.33 714 RASSF1 9.35 10.69 715 IL1RAPL1 6.42
8.18 716 AUH 9.37 11.19 717 DIAPH1 11.52 9.74 718 ENSA 12.33 11.22
719 FAM43A 10.62 9.39 720 CSMD3 4.79 7.58 721 SLC25A37 10.10 8.69
722 SLC33A1 9.78 12.19 723 CNOT6 9.98 8.93 724 GRID1 7.05 8.80 725
C21orf91 11.51 10.18 726 ITPKB 11.28 8.91 727 CP 5.89 7.41 728
HMBOX1 10.05 8.50 729 ARID1A 12.22 11.11 730 C5orf5 11.86 10.38 731
TMSB4X 16.86 15.10 732 EPAS1 7.02 8.39 733 HPS3 11.65 10.16 734
DKK1 6.16 7.86 735 ZNF282 7.89 5.40 736 AP3D1 11.29 12.84 737 DERL1
11.69 13.43 738 C5orf13 9.89 7.62 739 CCNG2 13.32 11.64 740 PGRMC2
9.44 11.38 741 ARF6 13.60 12.55
742 TMEM110 7.75 9.24 743 FAM13A1 7.11 9.69 744 BRMS1L 8.58 10.18
745 TRAM1 12.52 15.17 746 CALU 9.42 11.20 747 GOLPH3 11.55 12.81
748 MAP3K7 11.14 10.06 749 ABCG4 6.90 8.22 750 NELF 8.75 7.70 751
ADIPOR1 9.67 10.92 752 INSR 8.48 11.62 753 GRM5 6.24 7.65 754 TFG
10.76 12.30 755 USP48 11.91 13.09 756 CLCN6 9.26 7.48 757 ZNF219
6.11 8.42 758 C7orf43 8.34 6.62 759 NFKB1 11.90 10.70 760 ARF3
11.11 9.87 761 JMJD1C 12.34 11.11 762 ROD1 13.13 11.42 763 OXSR1
10.69 9.15 764 ERGIC2 10.86 12.81 765 EIF4E3 7.61 9.87 766 SEC24A
10.05 12.85 767 SPRED1 7.19 5.77 768 HNRPH3 12.60 11.38 769 ZDHHC7
10.34 9.05 770 HNRPA1 16.14 15.14 771 TMED2 12.01 14.26 772 VASP
10.60 8.60 773 CHD4 11.40 10.31 774 RTN4RL1 5.48 8.36 775 PPP1R12A
12.03 10.97 776 SGK3 9.58 11.68 777 ARHGAP17 12.37 8.62 778 GORASP2
11.28 13.30 779 C2orf44 8.38 6.72 780 FAM116A 11.12 10.08 781 SSR1
12.75 14.73 782 MYH9 11.24 9.89 783 PIK3CD 12.99 10.03 784 DAZAP2
14.26 13.23 785 SEL1L 10.74 14.04 786 LOC388284 7.05 8.32 787 THRB
7.51 6.11 7.51 6.29 788 MGAT2 11.91 14.44 789 C8orf13 9.35 6.47 790
PDZD2 7.00 4.39 791 DERL2 10.74 12.75 792 ZCCHC5 7.39 6.27 793 NFYC
10.27 9.03 794 GLUD2 8.88 7.58 795 ESR1 7.55 9.88 796 NRP2 6.51
8.13 797 NUS1 10.01 11.53 798 BIRC6 12.15 10.94 799 ARID3B 7.83
9.22 800 STCH 10.16 13.88 801 SLC7A11 6.32 8.00 802 OSBPL5 7.91
9.22 7.91 6.62 803 IGFBP3 5.60 7.61 804 LNPEP 13.72 11.05 805 ZNRF1
8.18 9.68 806 DDAH1 7.96 5.87 807 ERG 4.07 7.49 808 APLP2 10.90
8.38 809 ZNFX1 10.14 8.93 810 GPM6B 8.66 6.69 811 BLCAP 11.49 10.10
812 SRP68 11.47 12.88 813 PIK3AP1 14.12 11.74 814 ANKRD28 11.00
12.46 815 KCNH8 8.27 4.80 816 KIAA0430 12.43 10.61 817 PPP2R1B 9.84
8.41 818 TMED9 11.62 13.82 819 CTNNBIP1 7.31 8.89 820 PPP2R5D 9.51
7.92 821 CBX4 12.36 13.69 822 AKAP6 7.38 5.55 7.38 4.93 823 C2orf30
10.72 13.84 824 DR1 11.97 10.65 825 MTDH 12.46 13.96 826 ETS1 14.40
12.62 827 EPHA8 6.12 8.10 828 ANKRD13B 7.23 5.85 829 C4orf16 8.83
7.70 830 SOX4 9.14 10.20 831 SLC12A2 10.23 9.01 832 IER3IP1 11.35
12.56 833 DST 7.02 8.34 834 WRNIP1 11.03 9.49 835 CLCC1 9.63 11.48
836 B4GALT6 7.90 6.82 837 BTBD10 10.12 8.07 838 SDC1 7.05 12.14 839
C10orf12 10.32 8.40 840 ZDHHC3 9.80 8.78 841 GNAI2 10.86 9.27 842
HNRPU 13.82 12.47 843 GNAZ 8.86 7.26 844 ALG9 8.93 10.97 845 FBXL10
11.75 10.43 846 ARID4B 12.19 10.96 847 PPP1R3F 6.19 7.79 848 EPB49
6.49 7.94 849 PCNP 12.91 11.74 850 PCYT1B 11.35 9.77 851 RAB14
11.64 10.41 852 TCERG1 12.50 11.46 853 MKNK2 14.87 12.67 854 COL4A3
8.78 12.23 855 HNRPA0 12.50 11.36 856 P2RX4 8.88 10.29 857 JUP 9.23
5.70 858 EGR2 10.43 8.07 859 SUPT16H 11.35 9.60 860 PNRC2 13.93
11.64 861 SRPR 11.87 13.89 862 BPTF 12.46 11.34 863 RBM16 12.05
10.52 864 YWHAZ 13.62 12.61 865 EDEM3 11.01 12.76 866 C12orf23
11.18 12.40 867 DOC2A 3.96 7.21 868 SETD2 11.54 10.39 869 FAM98A
10.91 12.10 870 C13orf18 13.25 9.86 871 BRP44L 10.48 11.91 872
CCDC6 11.25 9.82 873 GLCCI1 12.22 15.19 874 PLEKHH1 7.03 9.25 875
SMEK1 11.38 10.37 876 ANKRD9 4.70 8.61 877 CSNK1E 8.86 11.48 878
DCUN1D3 7.48 5.95 879 LTB 14.66 9.91 880 EOMES 7.30 6.14 881 PARP8
10.81 8.69 882 ABCD1 7.12 8.49 883 HOXA10 5.56 7.32 884 RNPS1 12.60
11.25 885 AYTL2 12.08 10.79 886 FLJ20699 5.74 8.80 887 SAPS1 11.43
9.76 888 SYNJ1 8.36 9.64 889 ZNF403 12.06 10.91 890 KPNB1 13.37
12.26 891 ISOC1 9.67 10.98 892 HYOU1 9.95 11.85 893 ZNF706 12.83
14.30 894 HDLBP 10.60 13.37 895 ELF1 12.70 11.34 896 TM9SF4 8.66
10.55 897 RAB8A 11.94 10.88 898 MORF4L2 12.94 14.69 899 SERP1 13.31
15.16 900 ADAMTS5 5.71 7.31 901 TRIOBP 11.23 10.18 902 ZNF664 11.32
12.57 903 RAB11FIP1 11.78 9.03 904 MYO18A 8.06 6.19 905 DUSP10
10.13 8.70 906 ATXN1 6.66 10.65 6.66 10.25 907 EPB41L4B 6.12 7.95
908 CNTNAP1 6.51 9.06 909 SLC17A7 6.47 8.01 910 SPCS2 13.75 15.53
911 CCNC 12.68 14.10 912 WDR77 10.67 8.83 913 GMFB 12.03 10.70 914
ITGA6 6.72 10.51 915 CASP8AP2 10.55 9.44 916 SEC24D 8.79 11.88 917
CBX1 12.16 10.96 918 TBX3 6.05 7.89 919 NFAT5 12.09 10.72 920
ATP6V1B2 10.73 9.53 921 ACTG1 16.72 14.81 922 RND3 7.31 5.65 923
HNRPUL1 11.97 10.58 924 TJP1 7.18 10.59 925 RAPGEF4 5.25 8.09 926
RGMA 5.65 7.42 927 NAP1L1 14.70 12.60 928 TARDBP 12.72 11.49 929
DAZAP1 12.74 11.30 930 ZNF609 8.26 6.94 8.26 9.52 931 ARL1 10.44
12.41 932 ERC2 7.03 5.97 933 PDE4A 7.65 9.22 934 CSK 12.83 10.59
935 IMPAD1 8.44 10.05 936 PNN 13.75 12.48 937 DCP2 13.06 10.74 938
PIK3R3 6.34 7.82 939 RAB1A 13.59 14.83 940 CENTB2 12.39 10.97 941
SPTAN1 11.48 9.79 942 DNAJC8 12.25 11.00 943 UNC13B 6.70 8.35 944
DHX15 14.19 12.99 945 VEZF1 11.45 9.94 946 DHDDS 8.74 10.05 947
FAM55C 8.19 9.74 948 USP37 8.89 7.60 949 MMD 11.68 10.32 950 HCLS1
14.42 13.12 951 KIAA1370 13.30 14.72 952 GANC 8.31 6.12 953 SSRP1
11.34 10.27 954 G3BP1 12.24 10.55 955 BAAT 4.91 7.24 956 FNDC3B
8.27 14.34 957 FBXW7 10.21 12.19 958 SPECC1L 10.27 7.66 959 YBX1
15.31 14.20 960 YAP1 7.23 5.61 961 ARF4 12.09 14.23 962 PNKD 10.86
9.79 963 CLEC2D 11.96 10.90 964 XPO1 13.81 11.82 965 TRAM2 9.38
12.80 966 MITF 8.05 6.41 967 CFL1 16.37 14.09 968 CREB5 5.46 7.24
969 GREM1 4.48 7.14 970 LONRF1 11.26 10.00 971 NME7 9.37 8.30 972
PMAIP1 12.26 10.75 973 KLHL14 11.89 10.84 974 AOF1 9.33 7.96 9.33
8.28 975 SMPD3 5.90 7.83 976 RGL1 6.66 8.04 977 LYPD6 5.38 7.39 978
STAT5B 10.95 9.73 979 C10orf6 10.04 8.44 980 CLDND1 10.72 12.01 981
DUSP9 5.59 7.70 982 SLC36A1 8.53 7.23 983 PAM 8.90 10.86 984 GHR
5.87 7.56 985 CBL 11.69 10.26 986 CENTG2 5.96 7.39 987 BACH2 13.68
9.35 13.68 11.88 988 NUP153 12.33 10.55 989 CPD 8.37 9.72 990 APLN
8.58 6.47 991 RFXDC2 11.67 10.00 992 GABRB2 5.58 8.10
993 NOMO3 5.39 7.39 994 RHOJ 6.41 7.75 995 PRDM1 9.15 14.10 996
IGSF3 7.62 5.91 997 UBE2A 12.47 11.47 998 KBTBD8 12.82 9.73 12.82
11.71 999 PRR15 7.27 5.94 7.27 4.67 1000 ILF3 11.94 10.71 1001
WIPF1 13.81 12.41 1002 GCNT2 11.01 8.49 1003 DBNL 10.43 8.48 1004
C14orf43 10.14 8.01 1005 MID1IP1 9.16 8.11 1006 LIMD2 10.43 8.88
1007 HNRPK 14.49 12.95 14.49 13.46 1008 ZNF697 5.74 8.07 1009 TSHZ3
7.31 5.32 1010 MBNL2 11.88 13.47 1011 ELL 7.83 9.17 1012 MAFB 7.30
8.56 1013 GGA2 13.25 10.77 1014 C20orf121 10.01 8.93 1015 CDC2L6
11.52 9.69 1016 TOB2 11.39 9.58 1017 MAP4K4 12.04 10.41 1018
FAM102A 11.41 10.18 1019 ITGB3BP 11.86 10.76 1020 HIVEP1 10.35 9.05
1021 NUDT21 12.51 11.11 1022 EIF4A1 14.02 12.98 1023 TSPAN14 8.38
6.31 1024 SLC2A4RG 8.98 7.40 1025 DGKZ 9.74 7.85 1026 PTPN9 9.30
7.85 1027 BCL7A 11.33 10.10 1028 LSM14A 12.74 11.19 1029 GNB2 11.50
10.12 1030 SLC6A6 11.35 8.51 1031 TACC1 13.21 11.62 1032 LIMK2 9.80
8.12 1033 ACVR2B 6.69 8.33 1034 SCC-112 12.25 10.55 1035 PTBP1
13.38 12.19 13.38 12.21 1036 CITED2 9.88 12.56 1037 SNX5 14.17
13.16 1038 ACTR2 15.12 13.32 1039 OSBPL3 10.59 12.84 1040 SFRS1
14.60 13.24 1041 ADM 7.24 11.10 1042 PFKFB3 11.91 10.01 1043 FNBP1
13.61 12.25 1044 MTMR2 10.53 9.06 1045 DDHD1 10.18 8.74 1046 HSPA5
13.70 16.32 1047 PTK2 12.12 8.33 1048 UBE2I 13.17 11.22 1049 SNAP23
13.78 12.23 1050 MTERFD2 10.53 8.59 1051 COTL1 13.89 10.14 1052
PHACTR2 8.65 6.86 1053 GLRA2 5.93 7.69 1054 NUTF2 10.39 9.26 1055
TSGA14 9.52 8.03 1056 FKBP1A 12.16 10.89 12.16 10.82 1057 LRRC1
8.82 7.68 1058 PDIA6 13.07 14.91 1059 YWHAQ 14.50 12.99 1060 ACHE
5.59 8.29 1061 KRAS 12.36 10.49 1062 FAM107B 13.87 12.55 1063
CCDC117 11.03 9.56 1064 WDR1 13.38 11.36 1065 HMGB1 16.36 14.98
16.36 14.97 1066 CCND3 12.69 9.79 1067 SUV39H1 9.28 8.07 1068
RANBP5 11.94 10.87 1069 SLC25A33 10.24 9.00 1070 EDNRA 5.94 7.12
1071 RAB11A 12.94 11.73 1072 DDEF1 11.98 10.54 1073 CXorf15 11.84
10.47 1074 C4orf34 13.65 10.16 1075 SLC44A1 11.10 13.50 1076
EIF2AK3 12.29 13.83 1077 RAP1B 14.21 13.21 1078 CDK2 10.20 8.73
10.20 9.01 1079 JDP2 7.94 5.97 1080 GSTCD 8.86 7.85 1081 TNKS2
10.95 9.62 10.95 9.62 1082 FUZ 7.15 8.96 7.15 8.19 1083 SYPL1 13.56
10.87 1084 EIF4H 13.07 12.02 1085 PIP5K2A 9.65 8.06 1086 RGS2 11.61
14.48 1087 ARF5 10.35 9.13 1088 JAKMIP2 8.57 6.96 1089 RABGAP1
12.19 9.65 12.19 10.85 1090 LRIG1 9.76 10.77 10.77 9.16 1091 CLTA
13.08 11.71 1092 APBB2 7.87 6.39 1093 SMC1A 12.88 11.33 1094 CDC27
9.86 10.88 1095 STT3A 8.64 9.66 1096 ZFAND6 12.01 13.04 13.04 11.95
1097 BZRAP1 8.43 7.15 1098 DPP3 9.41 10.44 10.44 8.39 1099 CNIH
11.94 12.98 1100 TMEM16F 9.42 10.46 1101 ARHGEF7 11.99 13.03 13.03
11.18 13.03 11.60 1102 CAMTA1 10.60 11.64 1103 PFN1 14.03 15.08
1104 USP12 10.18 11.23 1105 AZIN1 11.24 12.29 1106 GNA13 13.89
14.94 14.94 13.37 1107 RCOR1 10.46 11.51 11.51 9.96 1108 USP1 13.36
11.52 1109 VANGL1 7.47 9.15 1110 CAPZB 11.85 12.91 1111 CDV3 13.86
14.91 1112 ABI1 11.61 12.67 12.67 10.96 1113 ST5 7.21 8.28 1114 TTL
8.63 9.69 9.69 8.03 1115 ANP32E 12.76 13.82 13.82 12.19 1116 USP7
12.27 13.34 1117 BCL11B 7.20 8.27 8.27 9.68 1118 OAZ2 8.98 10.06
1119 PPP2CA 12.04 13.12 13.12 11.89 1120 TSC1 10.58 11.66 1121
CTDSPL2 9.81 10.90 10.90 9.51 10.90 9.68 1122 NEDD4L 7.81 8.90 1123
NAT13 12.06 13.16 1124 ASF1A 9.54 10.64 1125 DPF1 7.18 8.50 7.18
5.84 1126 RAN 13.46 14.57 14.57 12.39 1127 ELAVL1 11.66 12.77 12.77
11.43 1128 GRHL1 5.76 7.24 1129 SMAD2 10.45 11.57 1130 IQWD1 9.70
10.83 10.83 9.73 1131 ETS2 7.50 5.66 1132 CDC25B 10.04 11.18 1133
USP32 10.65 11.78 11.78 10.75 1134 EED 11.76 12.90 12.90 11.50 1135
CTNND2 5.93 7.81 1136 SHROOM3 7.86 9.01 9.01 6.05 1137 PSCD3 6.43
7.57 1138 ATF7 7.83 8.97 1139 CDK6 10.31 11.46 1140 GCH1 12.59
10.17 1141 AP3S1 11.95 13.12 1142 ARL6IP1 12.03 13.20 13.20 11.51
1143 TBC1D1 11.00 12.17 12.17 10.01 1144 ATP2A2 10.75 11.92 11.92
10.76 1145 SORL1 12.49 13.67 13.67 10.58 1146 SOX5 9.30 10.47 10.47
7.33 10.47 9.08 1147 KIAA1411 8.65 9.83 1148 KIAA0922 12.43 13.61
13.61 10.86 13.61 12.35 1149 SSX2IP 7.82 9.00 9.00 7.49 1150 SNRPD1
11.85 13.04 1151 EDD1 11.23 12.42 1152 BLMH 9.01 10.21 1153 PTPLB
11.32 12.52 1154 SLC25A27 7.44 3.28 1155 PGAM1 12.40 13.61 13.61
12.55 1156 HMGA1 11.26 12.47 12.47 10.36 1157 EDEM1 11.34 12.55
1158 PRPF38A 10.27 11.48 11.48 10.00 1159 DCUN1D1 9.82 11.03 1160
ROBO1 7.43 8.64 1161 LRRC59 11.26 12.65 1162 ZNF207 12.29 13.51
1163 GTDC1 9.19 10.42 10.42 9.35 1164 C1orf121 10.47 11.70 1165 NF1
9.23 10.46 1166 CUL3 12.98 14.21 14.21 12.80 14.21 12.99 1167
B4GALT5 8.74 9.97 9.97 8.08 9.97 8.71 1168 GADD45A 8.14 9.37 9.37
11.74 1169 RASD2 5.53 7.35 1170 CD4 6.76 8.01 8.01 6.11 1171 WWC1
6.16 7.88 1172 DUSP2 9.43 10.69 10.69 9.18 1173 TP53INP2 8.44 9.70
9.70 8.53 9.70 8.00 1174 NRAS 8.89 10.16 1175 TFRC 12.12 13.39
13.39 11.88 1176 MASTL 8.76 10.03 10.03 8.23 10.03 8.64 1177 USP6
9.12 10.39 10.39 9.25 1178 VGLL4 10.30 11.57 11.57 10.16 1179
C10orf78 8.93 10.21 1180 BTG3 9.87 11.16 1181 TMOD2 8.64 9.93 9.93
6.94 1182 HOXA5 7.03 8.32 1183 AK2 10.33 11.62 1184 MAP2K1 10.41
11.71 11.71 10.69 11.71 10.61 1185 CASP3 10.16 11.47 1186 STK40
9.30 10.60 10.60 8.13 1187 GRHL3 5.20 7.46 1188 SLC25A4 8.65 9.96
1189 KLHDC5 9.52 10.83 1190 SAMHD1 9.72 11.03 1191 PHLPP 7.93 9.24
9.24 7.58 1192 CCNE1 7.86 9.18 1193 EGR1 11.92 13.24 1194 PIM1 9.44
10.76 10.76 8.87 1195 HECW1 6.49 7.81 1196 CHAC1 6.73 8.76 1197
P4HA2 6.84 8.18 1198 PPM1E 6.59 7.92 1199 KIAA0746 13.65 14.98 1200
LOC401720 6.33 7.63 1201 CAPN5 5.90 7.24 1202 DYNLL1 12.69 14.03
1203 EHMT1 10.40 11.75 1204 RIC8B 7.54 8.89 1205 GRIN1 7.40 8.93
1206 DEK 12.73 14.09 14.09 10.29 1207 E2F1 7.01 8.37 8.37 7.21 1208
FAM45A 10.66 12.02 1209 VAMP1 11.10 12.47 1210 LTBP1 7.23 8.61 1211
SOCS1 9.18 10.56 10.56 9.25 1212 ZCCHC14 6.98 8.36 1213 KLHL3 8.04
9.43 1214 RET 5.90 7.29 7.29 5.99 1215 CUTL1 10.33 11.72 11.72
10.67 11.72 10.50 1216 RBL1 7.58 8.98 8.98 7.52 8.98 7.97 1217 TOP1
11.47 12.87 1218 GPD1L 7.94 9.35 1219 SAR1B 9.03 10.46 10.46 12.55
1220 MTF2 11.84 13.27 13.27 11.18 1221 ANP32B 15.53 14.21 1222 CIT
8.19 9.63 9.63 7.43 9.63 7.77 1223 POU3F1 6.08 7.52 1224 MTMR12
11.44 12.89 12.89 11.13 12.89 11.60 1225 MBOAT2 6.73 8.17 8.17 6.25
1226 DOCK9 9.28 10.73 1227 ZAK 8.60 10.05 10.05 8.21 1228 LOC152485
11.05 12.51 12.51 10.56 1229 HNRPA3 14.33 12.26 1230 LMNB1 10.63
8.78 1231 ZFYVE21 8.42 9.90 1232 TXNDC5 12.47 16.40 1233 UBE2G1
11.36 12.84 1234 KIF23 7.44 8.93 8.93 5.57 8.93 6.64 1235 DPYSL2
9.98 11.46 11.46 9.03 1236 ATP5G3 12.15 13.64 1237 GLRX5 10.08
11.57 1238 NLK 10.41 11.92 1239 ARL6IP6 10.51 12.02 12.02 10.43
1240 CNNM4 6.17 7.68 7.68 5.21 1241 TBC1D4 9.12 10.64 10.64 8.94
1242 CD163 5.49 7.00 1243 PKD2 8.17 9.71 9.71 7.48
1244 DIAPH3 8.23 9.77 9.77 8.34 1245 RAD23B 10.65 12.20 12.20 10.54
1246 DCAMKL2 5.53 7.08 7.08 5.88 1247 LMBR1 7.22 8.77 1248 RRAS2
11.24 12.79 12.79 11.03 1249 MYO1D 6.31 7.88 7.88 10.07 1250 KLHL5
10.57 12.15 12.15 10.43 12.15 10.80 1251 EPS15 12.32 13.91 13.91
12.70 1252 FASLG 5.47 7.06 1253 H2AFY 12.56 14.16 1254 LIMA1 8.47
10.07 10.07 9.06 1255 CDCA4 8.92 10.52 10.52 9.16 1256 HAS3 5.70
7.31 7.31 6.15 1257 HRBL 5.96 7.57 7.57 6.35 7.57 5.97 1258 SYAP1
9.71 11.32 11.32 9.76 1259 MDFIC 12.06 13.67 1260 FAM76B 11.27
12.89 12.89 10.52 1261 SNTB2 8.22 9.85 9.85 8.64 1262 ARL3 8.92
10.55 1263 GPR124 5.50 7.14 1264 BCL2L11 10.79 12.43 12.43 11.14
1265 RNF103 12.03 13.23 1266 MYB 6.97 8.62 8.62 5.61 1267 PKM2
11.21 12.87 1268 VCL 8.92 10.57 10.57 8.25 1269 RBBP7 12.45 14.11
14.11 12.96 14.11 12.70 1270 LBR 12.86 14.53 14.53 12.16 14.53
12.50 1271 RRBP1 6.97 8.64 8.64 11.94 1272 GABRB3 5.33 7.00 1273
SGCB 7.78 9.45 9.45 8.22 1274 FAM81A 6.61 8.30 8.30 5.44 1275 RAB15
5.75 7.44 1276 SOX9 6.72 8.42 1277 SAP30 8.22 9.91 1278 BRWD1 8.60
10.30 10.30 8.72 10.30 9.04 1279 KCNMA1 7.47 9.17 9.17 10.42 1280
WHSC1 9.67 11.37 11.37 9.60 1281 CCDC126 9.36 11.09 1282 GRAMD1C
7.83 9.57 1283 PHF19 8.98 10.73 10.73 9.18 1284 ADAM23 7.02 8.77
8.77 7.00 1285 C9orf150 5.38 7.13 1286 ZNF572 6.52 8.30 8.30 6.82
1287 STK39 9.38 11.15 11.15 9.40 1288 SMS 9.73 11.54 1289 DMD 8.96
10.77 10.77 9.32 1290 C1orf83 6.15 7.96 1291 MFHAS1 9.80 11.61
11.61 9.36 1292 STXBP1 6.57 7.79 1293 CPNE2 5.44 7.27 7.27 4.84
1294 MYH10 7.07 8.90 8.90 7.85 1295 CALM3 9.63 11.46 11.46 10.30
1296 EFNB2 7.01 8.85 1297 ACTN1 5.89 7.74 1298 RBMS3 5.67 7.51 1299
ACOT7 9.70 7.83 1300 RKHD1 5.92 7.77 1301 LRRK1 9.74 11.62 11.62
10.12 1302 PTCH1 7.70 9.58 1303 MGLL 7.30 9.18 9.18 10.57 9.18 8.06
1304 YWHAH 8.94 10.82 10.82 9.09 1305 PDE4D 6.78 8.66 1306 MAF 8.05
9.95 9.95 8.25 1307 PTGER3 5.18 7.09 7.09 6.06 1308 PRKCD 9.49
11.40 1309 CCDC64 8.15 10.07 10.07 7.55 1310 RASL11A 7.89 6.65 1311
KPNA2 11.18 13.09 13.09 11.78 13.09 11.55 1312 GPR137B 8.65 10.58
10.58 9.36 1313 TIAM1 6.97 8.90 1314 TFDP1 10.95 12.90 12.90 10.49
1315 SSBP2 10.47 12.42 12.42 10.32 1316 REEP1 5.64 7.61 1317 MAP2
7.85 9.83 9.83 7.75 1318 HOXA9 5.15 7.12 1319 SCRN1 11.81 9.54 1320
LOC129607 8.79 10.78 10.78 9.33 10.78 9.45 1321 SIAH2 10.35 12.34
12.34 10.57 1322 DKFZP564O0823 6.96 8.95 1323 POLQ 8.30 10.30 10.30
8.98 10.30 7.83 1324 KLF15 5.88 7.88 7.88 9.36 7.88 6.74 1325 PXDN
8.36 10.37 1326 BTBD12 8.06 10.08 10.08 8.56 1327 PHF6 11.19 13.21
13.21 11.96 13.21 12.09 1328 SLC41A2 5.67 7.68 1329 HN1 9.87 11.92
11.92 9.85 11.92 10.58 1330 ZNF608 8.98 11.07 11.07 9.74 1331 RNGTT
10.32 12.45 1332 RAP2A 10.35 12.47 1333 LIMK1 5.18 7.36 7.36 5.05
1334 SMAD1 5.91 8.10 8.10 6.34 1335 NCOA7 10.39 12.58 1336 PRDM15
10.29 12.49 1337 PELI1 10.67 12.88 1338 PLS1 5.43 7.65 7.65 4.53
1339 RAB23 6.14 8.36 1340 NAP1L5 7.40 9.63 9.63 8.30 9.63 8.34 1341
DNER 6.90 9.14 1342 LRRC42 7.58 9.83 1343 ID2 7.74 9.99 1344 IBRDC2
9.98 12.24 12.24 8.84 12.24 9.94 1345 DNMT1 11.21 13.50 1346 STAC3
5.74 8.02 8.02 7.01 1347 HMGB3 8.20 10.50 10.50 8.80 10.50 8.08
1348 BMPR1A 6.75 9.06 1349 SGK 10.06 12.37 1350 CBX2 6.06 8.37 8.37
6.38 1351 LRRC20 5.69 8.03 1352 LRRC4 4.81 7.15 7.15 5.05 1353
HOXA1 5.36 7.70 1354 LRRC62 4.95 7.30 1355 ATAD2 9.00 11.34 11.34
8.44 11.34 9.37 1356 MOBKL1A 9.73 12.08 12.08 9.13 12.08 10.12 1357
LOC220594 10.06 12.41 12.41 10.07 1358 ZNF804A 6.14 8.51 1359
C1orf113 5.99 8.36 8.36 5.74 1360 FMNL2 6.58 8.96 1361 H2AFX 12.49
10.60 1362 ATP1B1 5.60 8.03 1363 GPT2 6.54 8.97 1364 PSRC1 9.89
7.46 1365 SLC25A35 5.23 7.68 1366 LHFPL2 8.94 11.41 1367 UBE2J1
11.34 13.81 13.81 12.22 1368 TBC1D8B 5.43 7.91 7.91 6.89 1369 SGPP1
10.96 13.46 13.46 11.28 1370 C11orf9 4.74 7.24 1371 BCL6 12.29
14.82 14.82 10.60 14.82 11.78 1372 ANUBL1 8.12 10.66 10.66 8.78
1373 MTA3 8.37 10.92 1374 PGBD5 4.87 7.45 7.45 5.87 1375 LPP 11.00
13.59 13.59 10.07 13.59 10.89 1376 NDFIP2 7.39 9.99 1377 STMN1
11.76 9.03 1378 PITPNC1 8.77 11.42 11.42 10.00 1379 SH3RF1 7.37
10.05 10.05 8.10 1380 ASF1B 8.56 11.25 11.25 7.91 11.25 8.27 1381
FLJ20186 12.06 14.82 14.82 12.05 1382 SLC16A2 8.08 5.17 1383 PEX5
9.12 11.90 11.90 9.50 1384 ECT2 7.45 10.23 10.23 7.70 10.23 7.63
1385 MAML3 8.05 10.85 1386 TEAD1 4.42 7.24 1387 HMGB2 12.96 15.78
15.78 12.73 15.78 13.36 1388 NCALD 5.15 7.97 1389 RGC32 7.75 10.62
1390 PPP1R3C 4.18 7.06 1391 DEPDC1B 9.96 7.11 1392 WEE1 10.11 13.01
13.01 10.32 13.01 11.84 1393 FHL2 4.82 7.77 1394 ITGB8 4.70 7.66
1395 SLC1A1 10.86 8.88 1396 FAM83D 7.36 10.32 1397 UHRF1 12.22 8.72
1398 C7orf41 4.94 7.95 7.95 6.29 1399 ZBTB8 5.58 8.59 8.59 11.07
1400 ZNF367 7.97 10.99 10.99 8.04 10.99 8.22 1401 CDC25A 6.03 9.09
9.09 6.81 1402 CHEK1 7.59 10.68 10.68 7.72 1403 CDCA7 10.61 13.76
13.76 10.16 13.76 11.06 1404 FGF13 4.55 7.69 1405 SSBP3 6.20 9.35
1406 EZH2 9.40 12.57 12.57 10.22 1407 TNFSF11 5.18 8.36 8.36 5.91
8.36 9.78 1408 PACSIN1 5.16 8.35 1409 RRM2 10.59 13.82 13.82 9.47
13.82 10.79 1410 POU4F1 5.62 8.85 1411 MYBL2 9.07 12.41 12.41 9.12
1412 KIAA1212 8.46 11.81 11.81 8.55 1413 CCNE2 7.30 10.66 10.66
8.02 10.66 8.03 1414 IGF2BP3 7.92 11.34 11.34 9.93 11.34 9.55 1415
PRKAR2B 6.17 9.66 9.66 8.16 1416 CADPS 5.04 8.58 8.58 6.18 1417
ANKRD15 6.97 10.52 10.52 7.58 1418 DAAM1 9.33 12.92 1419 MAP1B 4.05
7.68 7.68 5.03 7.68 6.23 1420 AFF2 6.32 10.00 1421 E2F7 5.66 9.37
9.37 6.53 9.37 6.89 1422 PRC1 8.37 12.08 1423 ENPP5 3.54 7.26 7.26
4.97 1424 E2F8 7.68 11.41 1425 MED12L 7.82 11.59 11.59 7.46 11.59
10.57 1426 LOC162073 6.30 10.08 1427 LRRC32 4.14 7.93 7.93 6.61
7.93 4.95 1428 DMXL2 6.57 10.38 10.38 6.61 10.38 7.76 1429 FLJ20366
7.86 11.68 11.68 7.62 11.68 9.86 1430 TOX 9.05 12.88 12.88 11.27
1431 MME 11.56 10.33 1432 FGD6 7.63 11.73 11.73 9.12 1433 MYBL1
9.68 14.13 14.13 10.02 14.13 9.72 *Empty cells indicate values not
appreciably measured.
TABLE-US-00022 TABLE 16 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from germinal center B-cell (GCB) DLBC
Higher ABC vs GCB in hsa-miR-93/mmu-miR-93/rno-miR-93 GCB
hsa-miR-331-3p/mmu-miR-331-3p/rno-miR-331 GCB hsa-miR-129* GCB
hsa-miR-423-3p/mmu-miR-423-3p/rno-miR-423 GCB
hsa-miR-28-5p/mmu-miR-28/rno-miR-28 GCB mghv-miR-M1-7-3p GCB
hsa-miR-518b GCB ebv-miR-BHRF1-1 GCB
hsa-miR-140-5p/mmu-miR-140/rno-miR-140 GCB hsa-miR-505* GCB
hsa-miR-675 GCB hsa-miR-198 GCB
hsa-miR-125b-1*/mmu-miR-125b-3p/mo-miR- GCB 125b-3p
hsa-miR-361-5p/mmu-miR-361/rno-miR-361 GCB ebv-miR-BART8* GCB
TABLE-US-00023 TABLE 17 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from Burkitt lymphoma Higher ABC vs BL
in hsa-miR-155 ABC hsa-miR-29c/mmu-miR- ABC 29c/rno-miR-29c
hsa-miR-146b-5p/mmu-miR- ABC 146b/rno-miR-146b hsa-miR-29b/mmu-miR-
ABC 29b/rno-miR-29b hsa-miR-22/mmu-miR- ABC 22/rno-miR-22
hsa-miR-21/mmu-miR- ABC 21/rno-miR-21 hsa-miR-768-3p ABC
hsa-miR-145/mmu-miR- ABC 145/rno-miR-145 hsa-miR-29a/mmu-miR- ABC
29a/rno-miR-29a hsa-miR-30e/mmu-miR- ABC 30e/rno-miR-30e
hsa-miR-26a/mmu-miR- ABC 26a/rno-miR-26a hsa-miR-101/mmu-miR- ABC
101a/rno-miR-101a hsa-miR-24/mmu-miR- ABC 24/rno-miR-24
hsa-miR-26b/mmu-miR- ABC 26b/rno-miR-26b hsa-miR-27a/mmu-miR- ABC
27a/rno-miR-27a hsa-miR-27b/mmu-miR- ABC 27b/rno-miR-27b
hsa-miR-23b/mmu-miR- ABC 23b/rno-miR-23b hsa-miR-23a/mmu-miR- ABC
23a/rno-miR-23a hsa-miR-125b/mmu-miR- ABC 125b-5p/rno-miR-125b-5p
hsa-miR-30a/mmu-miR- ABC 30a/rno-miR-30a hsa-miR-142-3p/mmu-miR-
ABC 142-3p/rno-miR-142-3p hsa-let-7a/mmu-let-7a/rno-let- ABC 7a
hsa-miR-30b/mmu-miR- ABC 30b/rno-miR-30b-5p hsa-miR-142-5p/mmu-miR-
ABC 142-5p/rno-miR-142-5p hsa-miR-34b/mmu-miR-34b-3p ABC
hsa-miR-16/mmu-miR-16/rno- ABC miR-16 hsa-miR-30c/mmu-miR- ABC
30c/rno-miR-30c hsa-let-7c/mmu-let-7c/rno-let- ABC 7c hsa-miR-550
ABC hsa-miR-921 BL hsa-miR-30c-2*/mmu-miR- BL 30c-2*/rno-miR-30c-2*
hsa-miR-933 BL hsa-miR-658 BL hsa-miR-628-3p BL hsa-miR-503 BL
hsa-miR-193a-5p BL hsa-miR-30b* BL hsa-miR-93/mmu-miR-93/rno- BL
miR-93 hsa-miR-18b BL hsa-miR-18a/mmu-miR- BL 18a/rno-miR-18a
hsa-miR-874/mmu-miR- BL 874/rno-miR-874
TABLE-US-00024 TABLE 18 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from chronic lymphocytic leukemia
Higher ABC vs CLL in hsa-miR-125b/mmu-miR- ABC
125b-5p/rno-miR-125b-5p hsa-miR-126/mmu-miR- ABC 126-3p/rno-miR-126
hsa-miR-199a-3p/hsa- ABC miR-199b-3p/mmu-miR- 199a-3p/mmu-miR-
199b/rno-miR-199a-3p hsa-miR-145/mmu-miR- ABC 145/rno-miR-145
hsa-miR-143/mmu-miR- ABC 143/rno-miR-143 hsa-miR-637 ABC
hsa-miR-371-5p ABC kshv-miR-K12-6-3p ABC hsa-miR-628-3p ABC
hsa-miR-126*/mmu-miR- ABC 126-5p/rno-miR-126* hsa-miR-193a-5p ABC
hsa-miR-21/mmu-miR- ABC 21/rno-miR-21 hsa-miR-24/mmu-miR- ABC
24/rno-miR-24 hsa-miR-503 ABC hsa-miR-23a/mmu-miR- ABC
23a/rno-miR-23a hsa-miR-23b/mmu-miR- ABC 23b/rno-miR-23b
hsa-miR-22/mmu-miR- ABC 22/rno-miR-22 hsa-miR-665 ABC
hsa-let-7c/mmu-let-7c/rno- ABC let-7c hsa-miR-658 ABC
hsa-let-7a/mmu-let-7a/rno- ABC let-7a mghv-miR-M1-4 ABC hsa-miR-933
ABC hsa-miR-550 CLL hsa-miR-30e/mmu-miR- CLL 30e/rno-miR-30e
hsa-miR-142- CLL 5p/mmu-miR-142- 5p/rno-miR-142-5p hsa-miR-101/mmu-
CLL miR-101a/rno-miR- 101a hsa-miR-185/mmu- CLL miR-185/rno-miR-185
hsa-miR-888* CLL hsa-miR-199a- CLL 5p/mmu-miR-199a-
5p/rno-miR-199a-5p hsa-miR-668/mmu- CLL miR-668 hsa-miR-549 CLL
hsa-miR-801/mmu- CLL miR-801 hsa-miR-649 CLL hsa-miR-625* CLL
hsa-miR-140- CLL 3p/mmu-miR- 140*/rno-miR-140* hsa-let-7f/mmu-let-
CLL 7f/rno-let-7f hsa-miR-768-5p CLL hsa-miR-24-1*/mmu- CLL
miR-24-1*/rno-miR- 24-1* ebv-miR-BART13 CLL hsa-miR-339- CLL
5p/mmu-miR-339- 5p/rno-miR-339-5p hsa-miR-20b/mmu- CLL
miR-20b/rno-miR- 20b-5p hsa-miR-335/mmu- CLL miR-335-5p/rno-miR-
335 mghv-miR-M1-8 CLL hsa-miR-30d/mmu- CLL miR-30d/rno-miR-30d
hsa-miR-363/mmu- CLL miR-363/rno-miR-363 hsa-miR-361-3p CLL
hsa-miR-107/mmu- CLL miR-107/rno-miR-107 hsa-miR-154/mmu- CLL
miR-154/rno-miR-154 hsa-miR-638 CLL hsa-miR-340/mmu- CLL
miR-340-5p/rno-miR- 340-5p hsa-miR-331- CLL 3p/mmu-miR-331-
3p/rno-miR-331 hsa-miR-151- CLL 5p/mmu-miR-151- 5p/rno-miR-151
hsa-miR-636 CLL hsa-miR-33a/mmu- CLL miR-33/rno-miR-33 hsa-miR-486-
CLL 5p/mmu-miR-486 hsa-miR-150/mmu- CLL miR-150/rno-miR- 150
hsa-miR-25/mmu- CLL miR-25/rno-miR-25 hsa-miR-331- CLL
5p/mmu-miR-331-5p hsa-miR-299- CLL 5p/mmu-miR- 299*/rno-miR-299
hsa-miR-891a CLL hsa-miR-144* CLL hsa-miR-363*/rno- CLL miR-363*
hsa-miR-93/mmu- CLL miR-93/rno-miR-93 hsa-miR-423- CLL
3p/mmu-miR-423- 3p/rno-miR-423 hsa-let-7g/mmu-let- CLL 7g
hsa-miR-28-5p/mmu- CLL miR-28/rno-miR-28 hsa-miR-20b* CLL
hsa-miR-140- CLL 5p/mmu-miR- 140/rno-miR-140 hsa-miR-519d CLL
hsa-miR-147 CLL hsa-miR-487b/mmu- CLL miR-487b/rno-miR- 487b
hsa-miR-361- CLL 5p/mmu-miR- 361/rno-miR-361 hsa-miR-186/mmu- CLL
miR-186/rno-miR- 186 hsa-miR-32/mmu- CLL miR-32/rno-miR-32
hsa-miR-129* CLL hsa-miR-30e*/mmu- CLL miR-30e*/rno-miR- 30e*
hsa-miR-196a*/mmu- CLL miR-196a*/rno-miR- 196a*
TABLE-US-00025 TABLE 19 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from follicular lymphoma Higher ABC vs
FL in hsa-miR-938 ABC hsa-miR-183*/mmu-miR- ABC 183*
hsa-miR-197/mmu-miR- ABC 197 hsa-miR-382/mmu-miR- ABC
382/rno-miR-382 hsa-miR-20b* ABC hsa-miR-524-5p ABC hsa-miR-337-3p
ABC hsa-miR-600 ABC hsa-miR-96/mmu-miR- ABC 96/rno-miR-96
mghv-miR-M1-8 ABC hsa-miR-29c*/mmu-miR- ABC 29c*/rno-miR-29c*
hsa-miR-575 ABC hsa-miR-518a-3p ABC hsa-miR-361-5p/mmu-miR- ABC
361/rno-miR-361 hsa-miR-193b* ABC hsa-miR-340*/mmu-miR- ABC
340-3p/rno-miR-340-3p hsa-miR-708/mmu-miR- ABC 708/rno-miR-708
hsa-miR-129* ABC hsa-miR-525-5p ABC hsa-miR-497/mmu-miR- FL
497/rno-miR-497 hsa-miR-22*/mmu-miR- FL 22*/rno-miR-22*
hsa-miR-130b/mmu-miR- FL 130b/rno-miR-130b hsa-miR-551b* FL
hsa-miR-331-3p/mmu-miR- FL 331-3p/rno-miR-331 ebv-miR-BART13 FL
hsa-miR-877/mmu-miR- FL 877/rno-miR-877 hsa-miR-636 FL hsa-miR-922
FL hsa-miR-198 FL hsa-miR-342-5p/mmu-miR- FL 342-5p/rno-miR-342-5p
hsa-miR-585 FL ebv-miR-BART8* FL hsa-miR-617 FL hsa-miR-221* FL
hsa-miR-125b-1*/mmu- FL miR-125b-3p/rno-miR- 125b-3p
hsa-miR-93/mmu-miR- FL 93/rno-miR-93 hsa-miR-363*/rno-miR- FL 363*
hsa-miR-744/mmu-miR- FL 744 hsa-miR-659 FL hsa-miR-490-3p/mmu-miR-
FL 490 hsa-let-7d/mmu-let-7d/rno- FL let-7d hsa-miR-361-3p FL
ebv-miR-BHRF1-1 FL hsa-miR-92b/mmu-miR- FL 92b/rno-miR-92b
hsa-miR-151-5p/mmu-miR- FL 151-5p/rno-miR-151 hsa-miR-144* FL
hsa-miR-425/mmu-miR- FL 425/rno-miR-425 hsa-miR-138/mmu-miR- FL
138/rno-miR-138 hsa-miR-92a/mmu-miR- FL 92a/rno-miR-92a
hsa-miR-151-3p FL hsa-miR-25/mmu-miR- FL 25/rno-miR-25
hsa-miR-509-3-5p FL hsa-miR-30e*/mmu-miR- FL 30e*/rno-rniR-30e*
hsa-miR-28-5p/mmu-miR- FL 28/rno-miR-28 hsa-miR-200b*/mmu-miR- FL
200b* hsa-miR-148b/mmu-miR- FL 148b/rno-miR-148b-3p hsa-miR-488 FL
hsa-miR-99b/mmu-miR- FL 99b/rno-miR-99b hsa-miR-339-5p/mmu-miR- FL
339-5p/rno-miR-339-5p hsv1-miR-H1 FL hsa-miR-32/mmu-miR- FL
32/rno-miR-32 hsa-miR-885-5p FL hsa-miR-630 FL ebv-miR-BART16 FL
hsa-miR-505* FL hsa-miR-374b* FL hsa-miR-574-3p/mmu-miR- FL 574-3p
hsa-miR-874/mmu-miR- FL 874/rno-miR-874 hsa-miR-423-3p/mmu-miR- FL
423-3p/rno-miR-423 hsa-miR-889 FL hcmv-miR-UL148D FL
hsa-miR-487b/mmu-miR- FL 487b/rno-miR-487b hsa-miR-552 FL
hsa-miR-220b FL hsa-miR-551a FL hsa-let-7d*/mmu-let- FL
7d*/rno-let-7d* kshv-miR-K12-5 FL hsa-miR-629* FL
hsa-miR-99b*/mmu-miR- FL 99b*/rno-miR-99b* hsa-miR-615-3p/mmu-miR-
FL 615-3p hsa-miR-657 FL hsa-miR-301a/mmu-miR- FL 301a/rno-miR-301a
hsa-miR-518b FL hsa-miR-194/mmu- FL miR-194/rno-miR- 194
hsa-miR-647 FL kshv-miR-K12-6-5p FL hsa-miR-622 FL hsa-miR-516b FL
hsa-miR-675 FL hsa-miR-526b FL hsa-miR-671- FL 5p/mmu-miR-671-5p
hsa-miR-18a/mmu- FL miR-18a/rno-miR- 18a hsa-miR-18b FL
hsa-miR-181b/mmu- FL miR-181b/rno-miR- 181b hsa-miR-215 FL
hsa-miR-153/mmu- FL miR-153/rno-miR- 153 hsa-miR-625 FL hsa-miR-510
FL hsa-miR-519d FL mghv-miR-M1-7-3p FL hsa-miR-485- FL
3p/mmu-miR-485* hsa-miR-483-5p FL hsa-miR-140- FL 5p/mmu-miR-
140/rno-miR-140 hsa-miR-921 FL hsa-miR-186/mmu- FL miR-186/rno-miR-
186 hsa-miR-196a*/mmu- FL miR-196a*/rno-miR- 196a* hsa-miR-381/mmu-
FL miR-381/rno-miR- 381 hsa-miR-620 FL hsa-miR-152/mmu- FL
miR-152/rno-miR- 152 hsa-miR-766 FL mghv-miR-M1-7-5p FL
hsa-miR-374b/mmu- FL miR-374/rno-miR- 374 hsa-let-7c/mmu-let- FL
7c/rno-let-7c kshv-miR-K12-8 FL mghv-miR-M1-3 FL hsa-miR-920 FL
hsa-miR-519e* FL hsa-miR-147 FL hsa-miR-424 FL hsa-miR-193b FL
ebv-miR-BART19-3p FL hsa-miR-146b-3p FL hsa-miR-30c/mmu- FL
miR-30c/rno-miR- 30c hsa-miR-30a/mmu- FL miR-30a/rno-miR- 30a
hsa-miR-939 FL hsa-let-7a/mmu-let- FL 7a/rno-let-7a hsa-miR-122* FL
hsa-miR-206/mmu- FL miR-206/rno-miR- 206 ebv-miR-BART18-3p FL
hsa-miR-183/mmu- FL miR-183/rno-miR- 183 hsa-miR-9*/mmu- FL
miR-9*/rno-miR-9* kshv-miR-K12-1 FL hsa-miR-34c- FL 5p/mmu-miR-
34c/rno-miR-34c hsa-miR-934 FL hsa-miR-890 FL hsa-miR-514 FL
hsa-miR-297/mmu- FL miR-297a hsa-miR-553 FL hsa-miR-765 FL
hsa-let-7b*/mmu-let- FL 7b*/rno-let-7b* hsa-miR-500* FL hsa-miR-601
FL ebv-miR-BHRF1-3 FL hsa-miR-296- FL 3p/mmu-miR-296-
3p/rno-miR-296 hsa-miR-574- FL 5p/mmu-miR-574-5p hsa-miR-409- FL
5p/mmu-miR-409- 5p/rno-miR-409-5p hsa-miR-195* FL hsa-miR-635 FL
hsa-miR-542- FL 3p/mmu-miR-542- 3p/rno-miR-542-3p hcmv-miR-US25-1*
FL mghv-miR-M1-2 FL hsa-miR-509-5p FL hsa-miR-340/mmu- FL
miR-340-5p/rno- miR-340-5p hsa-miR-891a FL hsa-miR-23a/mmu- FL
miR-23a/rno-miR- 23a hsa-miR-19b/mmu- FL miR-19b/rno-miR- 19b
hsa-miR-515-5p FL hsa-miR-23b/mmu- FL miR-23b/rno-miR- 23b
hsa-miR-498 FL hsa-miR-886-5p FL hsa-miR-220c FL hsa-miR-10a/mmu-
FL miR-10a/rno-miR- 10a-5p hsa-miR-32* FL hsa-miR-24/mmu- FL
miR-24/rno-miR-24 hsa-miR-98/mmu- FL miR-98/rno-miR-98
hsa-let-7g/mmu-let-7g FL hsa-miR-302a/mmu- FL miR-302a hsa-miR-625*
FL hsa-miR-30b* FL hsa-miR-30b/mmu- FL miR-30b/rno-miR- 30b-5p
hsa-miR-377* FL hsa-miR-106b*/mmu- FL miR-106b*/rno-miR- 106b*
hsa-miR-181a-2* FL hsa-miR-887 FL hsa-miR-208a/mmu- FL
miR-208a/rno-miR- 208 ebv-miR-BART7* FL hsa-miR-7-2* FL
hsa-miR-155* FL hsa-miR-513a-3p FL kshv-miR-K12-7 FL hsa-miR-299-3p
FL hsa-miR-218-2*/mmu- FL miR-218-2*/rno-miR- 218*
hsa-miR-130b*/mmu- FL miR-130b* hsa-miR-620 FL hsa-miR-33a/mmu- FL
miR-33/rno-miR-33 hsa-miR-7/mmu-miR- FL 7a/rno-miR-7a
ebv-miR-BART6-3p FL hsa-miR-22/mmu- FL miR-22/rno-miR-22
hsa-miR-199b-5p FL hsa-miR-768-3p FL hsa-miR-494/mmu- FL
miR-494/rno-miR-494 hsa-miR-602 FL hsa-miR-125b-2*/rno- FL
miR-125b* hsa-miR-300 FL hsa-let-7e/mmu-let- FL 7e/rno-let-7e
hsa-miR-298 FL hsa-miR-576-3p FL hsa-miR-187* FL hsa-miR-365/mmu-
FL miR-365/rno-miR-365 hsa-miR-518a-5p/hsa- FL miR-527
hsa-miR-302d* FL hsa-miR-105 FL hsa-miR-126/mmu- FL
miR-126-3p/rno-miR- 126 hsa-miR-107/mmu- FL miR-107/rno-miR-107
hsa-miR-299-5p/mmu- FL miR-299*/rno-miR- 299 hsa-miR-28-3p/rno- FL
miR-28* hsa-miR-21/mmu- FL miR-21/rno-miR-21 hsa-miR-27b/mmu- FL
miR-27b/rno-miR-27b hsa-miR-516a-5p FL hsa-miR-129-5p/mmu- FL
miR-129-5p/rno-miR- 129 hsa-miR-583 FL hsa-miR-483-3p FL
hsa-miR-326/mmu- FL miR-326/rno-miR-326 hsa-miR-548d-5p FL
hsa-miR-629 FL ebv-miR-BART5 FL hsa-miR-665 FL hsa-miR-493 FL
hsa-miR-484/mmu- FL miR-484/rno-miR-484 hsa-miR-645 FL hsa-miR-452
FL hsa-miR-518c* FL hsa-miR-24-2*/mmu- FL miR-24-2*/rno-miR- 24-2*
hsa-miR-124/mmu- FL miR-124/rno-miR-124 hsa-miR-184/mmu- FL
miR-184/rno-miR-184 hsa-miR-27a*/mmu- FL miR-27a*/rno-miR- 27a*
hsa-miR-25* FL hsa-miR-34b/mmu- FL miR-34b-3p ebv-miR-BART17-5p FL
hsa-miR-658 FL hsa-miR-212/mmu- FL miR-212/rno-miR-212
hsa-miR-99a/mmu- FL miR-99a/rno-miR-99a hsa-miR-801/mmu- FL miR-801
hsa-miR-491-3p FL hsa-miR-551b/mmu- FL miR-551b/rno-miR- 551b
hsa-miR-214/mmu- FL miR-214/rno-miR-214 hsa-miR-30e/mmu- FL
miR-30e/rno-miR-30e hsa-miR-888* FL hsa-miR-505/rno-miR- FL 505
hsa-miR-27a/mmu- FL miR-27a/rno-miR-27a hsa-miR-15a/mmu- FL miR-15a
hsa-miR-20a/mmu- FL miR-20a/rno-miR-20a kshv-miR-K12-3 FL
hsa-miR-455-3p FL hsa-miR-486-5p/mmu- FL miR-486
TABLE-US-00026 TABLE 20 Predictor microRNAs that distinguish
activated B-cell (ABC) DLBCL from Hodgkin's lymphoma Higher ABC vs
HL in hsa-miR-19b/mmu-miR- ABC 19b/rno-miR-19b hsa-miR-30b/mmu-miR-
ABC 30b/rno-miR-30b-5p hsa-miR-142-3p/mmu- ABC
miR-142-3p/rno-miR-142- 3p hsa-miR-768-3p ABC hsa-miR-30c/mmu-miR-
ABC 30c/rno-miR-30c hsa-miR-29b/mmu-miR- ABC 29b/rno-miR-29b
hsa-miR-24/mmu-miR- ABC 24/rno-miR-24 hsa-miR-22/mmu-miR- ABC
22/rno-miR-22 hsa-miR-30e/mmu-miR- ABC 30e/rno-miR-30e
hsa-miR-30a/mmu-miR- ABC 30a/rno-miR-30a hsa-miR-26b/mmu-miR- ABC
26b/rno-miR-26b hsa-miR-26a/mmu-miR- ABC 26a/rno-miR-26a
hsa-miR-15a/mmu-miR- ABC 15a hsa-miR-16/mmu-miR- ABC 16/rno-miR-16
hsa-miR-142-5p/mmu- ABC miR-142-5p/rno-miR-142- 5p
hsa-miR-29a/mmu-miR- ABC 29a/rno-miR-29a hsa-miR-101/mmu-miR- ABC
101a/rno-miR-101a hsa-miR-126/mmu-miR- ABC 126-3p/rno-miR-126
hsa-miR-451/mmu-miR- ABC 451/rno-miR-451 hsa-let-7a/mmu-let-7a/rno-
ABC let-7a hsa-miR-23b/mmu-miR- ABC 23b/rno-miR-23b
hsa-miR-21/mmu-miR- ABC 21/rno-miR-21 hsa-miR-29c/mmu-miR- ABC
29c/rno-miR-29c hsa-miR-20a/mmu-miR- ABC 20a/rno-miR-20a
hsa-miR-27b/mmu-miR- ABC 27b/rno-miR-27b hsa-miR-23a/mmu-miR- ABC
23a/rno-miR-23a hsa-miR-27a/mmu-miR- ABC 27a/rno-miR-27a
hsa-miR-550 ABC hsa-let-7c/mmu-let-7c/rno- ABC let-7c
hsa-miR-34b/mmu-miR- ABC 34b-3p hsa-miR-933 HL hsa-miR-30c-2*/mmu-
HL miR-30c-2*/rno-miR-30c- 2* hsa-miR-503 HL hsa-miR-765 HL
hsa-miR-658 HL hsa-miR-620 HL hsa-miR-921 HL hsa-miR-30b* HL
mghv-miR-M1-4 HL hsa-miR-939 HL hsa-miR-494/mmu-miR- HL
494/rno-miR-494 hsa-miR-32* HL hsa-miR-491-3p HL
hsa-miR-10a/mmu-miR- HL 10a/rno-miR-10a-5p hsa-miR-33a/mmu-miR- HL
33/rno-miR-33 hsa-miR-99a/mmu-miR- HL 99a/rno-miR-99a
hsa-miR-199b-5p HL hsa-miR-365/mmu-miR- HL 365/rno-miR-365
hsa-miR-520d-5p HL hsa-miR-518c* HL hsa-miR-32/mmu-miR- HL
32/rno-miR-32 hsa-miR-214/mmu-miR- HL 214/rno-miR-214
hsa-miR-98/mmu-miR- HL 98/rno-miR-98 hsa-miR-302d* HL
hsa-miR-30e*/mmu-miR- HL 30e*/rno-miR-30e* hsa-miR-374b/mmu-miR- HL
374/rno-miR-374 hsa-miR-29a*/mmu-miR- HL 29a*/rno-miR-29a*
hsa-miR-532-5p/mmu- HL miR-532-5p/rno-miR-532- 5p hsa-miR-149* HL
hsa-miR-422a HL ebv-miR-BHRF1-2 HL hsa-miR-634 HL hsa-miR-143* HL
hsa-miR-620 HL hsa-miR-660 HL hsa-miR-140-5p/mmu- HL
miR-140/rno-miR-140 hsa-miR-28-5p/mmu-miR- HL 28/rno-miR-28
hsa-miR-519c-5p/hsa- HL miR-519b-5p/hsa-miR-
523*/hsa-miR-518e*/hsa- miR-522*/hsa-miR-519a*
hsa-miR-505/rno-miR-505 HL hsa-miR-184/mmu-miR- HL 184/rno-miR-184
hsa-miR-107/mmu-miR- HL 107/rno-miR-107 hsa-miR-298 HL
hsa-miR-455-3p HL hsa-miR-638 HL hsa-miR-502-3p HL
hsa-miR-149/mmu-miR- HL 149 hsa-miR-583 HL hsa-miR-105 HL
hsa-miR-128/mmu-miR- HL 128/rno-miR-128 hsa-miR-656 HL
hsa-miR-497/mmu-miR- HL 497/rno-miR-497 hsa-miR-152/mmu-miR- HL
152/rno-miR-152 hsa-miR-151-5p/mmu- HL miR-151-5p/rno-miR-151
hsa-miR-148b/mmu-miR- HL 148b/rno-miR-148b-3p hsa-miR-300 HL
hsa-miR-144* HL hsa-miR-145*/mmu-miR- HL 145* hcmv-miR-UL70-3p HL
hsa-miR-28-3p/rno-miR- HL 28* hsa-miR-27a*/mmu-miR- HL
27a*/rno-miR-27a* hsa-miR-194/mmu-miR- HL 194/rno-miR-194
hsa-miR-130b*/mmu-miR- HL 130b* hsa-miR-548d-5p HL hsa-miR-937 HL
hsa-miR-7/mmu-miR- HL 7a/rno-miR-7a hsa-miR-518a-5p/hsa- HL miR-527
hsa-miR-323-3p/mmu- HL miR-323-3p/rno-miR-323 hsa-miR-215 HL
hsa-miR-513a-3p HL hsa-miR-595 HL hsa-miR-515-5p HL hsa-miR-483-3p
HL hsa-miR-330-5p/mmu- HL miR-330/rno-miR-330 hsa-miR-18b HL
hsa-miR-509-3p HL hsa-miR-151-3p HL hsa-miR-934 HL
hsa-miR-328/mmu-miR- HL 328/rno-miR-328 hsa-miR-187* HL
kshv-miR-K12-3 HL hsa-miR-373* HL hsa-miR-96/mmu-miR- HL
96/rno-miR-96 hsa-miR-186/mmu-miR- HL 186/rno-miR-186
hsa-miR-886-5p HL hsa-miR-424 HL hsa-miR-147 HL
hsa-miR-340/mmu-miR- HL 340-5p/rno-miR-340-5p hsa-miR-129-5p/mmu-
HL miR-129-5p/rno-miR-129 hsa-miR-25* HL hsa-miR-193b HL
hsa-miR-574-5p/mmu- HL miR-574-5p hsa-miR-589 HL
hsa-miR-339-5p/mmu- HL miR-339-5p/rno-miR- 339-5p
hsa-miR-34c-5p/mmu- HL miR-34c/rno-miR-34c hsa-miR-891a HL
hsa-miR-18a/mmu-miR- HL 18a/rno-miR-18a hsa-miR-196a*/mmu- HL
miR-196a*/rno-miR- 196a* hsa-miR-17*/rno-miR- HL 17-3p
hsa-miR-296-5p/mmu- HL miR-296-5p/rno-miR- 296* hsa-miR-25/mmu-miR-
HL 25/rno-miR-25 hsa-miR-509-5p HL hsa-miR-550* HL
hsa-miR-708/mmu-miR- HL 708/rno-miR-708 hsa-miR-146b-3p HL
hsa-miR-625* HL hsa-miR-210/mmu-miR- HL 210/rno-miR-210
hsa-miR-93/mmu-miR- HL 93/rno-miR-93 hsa-miR-548b-3p HL
hsa-miR-652/mmu-miR- HL 652/rno-miR-652 hsa-miR-153/mmu-miR- HL
153/rno-miR-153 mghv-miR-M1-3 HL hsa-miR-194* HL
hsa-miR-23a*/rno-miR- HL 23a* hsa-miR-943 HL hsa-let-7d/mmu-let- HL
7d/rno-let-7d hsa-miR-498 HL hsa-miR-381/mmu-miR- HL
381/rno-miR-381 hsa-miR-586 HL hsa-miR-137/mmu-miR- HL
137/rno-miR-137 hsa-miR-610 HL hsa-miR-920 HL
hsa-miR-936 HL hsa-miR-744/mmu-miR- HL 744 ebv-miR-BART5 HL
hsa-miR-21* HL hsa-miR-516a-5p HL hsa-miR-576-5p HL mghv-miR-M1-6
HL hsa-miR-425/mmu-miR- HL 425/rno-miR-425 hsa-miR-220c HL
hsa-miR-10a*/mmu-miR- HL 10a*/rno-miR-10a-3p hsa-miR-452 HL
hsa-miR-345 HL hsa-miR-29c*/mmu-miR- HL 29c*/rno-miR-29c*
hsa-miR-887 HL hsa-miR-7-2* HL hsa-miR-363*/rno-miR- HL 363*
hsa-miR-22*/mmu-miR- HL 22*/rno-miR-22* hsa-miR-922 HL hsa-miR-92b*
HL hsa-miR-526a/hsa-miR- HL 520c-5p/hsa-miR-518d- 5p
hsa-miR-574-3p/mmu- HL miR-574-3p hsa-miR-92a/mmu-miR- HL
92a/rno-miR-92a hsa-miR-423-3p/mmu- HL miR-423-3p/rno-miR-423
hsa-miR-526b HL hsa-miR-526b* HL ebv-miR-BART6-3p HL
hsa-miR-92b/mmu-miR- HL 92b/rno-miR-92b hsa-miR-519e* HL
hiv1-miR-H1 HL hsa-miR-623 HL hsa-miR-483-5p HL mghv-miR-M1-2 HL
mghv-miR-M1-7-3p HL hsa-miR-519e HL hsa-miR-361-5p/mmu- HL
miR-361/rno-miR-361 hsa-miR-650 HL hsa-miR-361-3p HL hsa-miR-374b*
HL kshv-miR-K12-8 HL hsa-miR-150*/mmu-miR- HL 150*
hsa-miR-425*/mmu-miR- HL 425* hsa-miR-135a*/mmu- HL miR-135a*
hsa-miR-612 HL hsa-miR-212/mmu-miR- HL 212/rno-miR-212
hsa-miR-125b-2*/rno- HL miR-125b* hcmv-miR-UL112 HL hsa-miR-500 HL
hsa-miR-502-5p HL ebv-miR-BART18-3p HL hsa-miR-625 HL
hsa-miR-138/mmu-miR- HL 138/rno-miR-138 hsa-miR-500* HL
hsa-miR-124*/mmu-miR- HL 124*/rno-miR-124* hsa-miR-516b HL
hsa-miR-30c-1*/mmu- HL miR-30c-1*/rno-miR- 30c-1*
hsa-miR-331-5p/mmu- HL miR-331-5p hsa-miR-510 HL hsa-miR-376a* HL
hsa-miR-640 HL hsa-miR-331-3p/mmu- HL miR-331-3p/rno-miR-331
hsa-miR-602 HL hsa-miR-485-3p/mmu- HL miR-485* hsa-miR-488 HL
hsa-miR-125a- HL 3p/mmu-miR-125a- 3p/rno-miR-125a-3p
hsa-miR-24-2*/mmu- HL miR-24-2*/rno-miR- 24-2* hsa-miR-484/mmu- HL
miR-484/rno-miR- 484 hsa-miR-106b*/mmu- HL miR-106b*/rno-miR- 106b*
hsa-miR-600 HL hsa-let-7b*/mmu-let- HL 7b*/rno-let-7b*
hsa-miR-302c* HL hsa-miR-20b* HL hsa-miR-524-5p HL hsa-miR-505* HL
hsa-miR-542- HL 5p/mmu-miR-542- 5p/rno-miR-542-5p hsa-miR-557 HL
hsa-miR-183/mmu- HL miR-183/rno-miR- 183 hsa-miR-122* HL
hsa-miR-675 HL hsv1-miR-H1 HL hsa-miR-99b/mmu- HL miR-99b/rno-miR-
99b hsa-miR-766 HL hsa-miR-409- HL 5p/mmu-miR-409-
5p/rno-miR-409-5p ebv-miR-BART20-3p HL hsa-miR-129* HL
mghv-miR-M1-7-5p HL hsa-miR-671- HL 5p/mmu-miR-671-5p hsa-miR-629
HL hsa-miR-553 HL hsa-let-7d*/mmu-let- HL 7d*/rno-let-7d*
hsa-miR-601 HL hsa-miR-645 HL hsa-miR-221* HL hsa-miR-874/mmu- HL
miR-874/rno-miR- 874 hsa-miR-890 HL hsa-miR-492 HL hsa-miR-629* HL
hsa-miR-635 HL hsa-miR-130b/mmu- HL miR-130b/rno-miR- 130b
hsa-miR-197/mmu- HL miR-197 hsa-miR-654-5p HL hsa-miR-518b HL
hsa-miR-889 HL hsa-miR-584 HL hsa-miR-198 HL hsa-miR-636 HL
hsa-miR-630 HL hsa-miR-490-5p HL hsa-miR-663 HL hcmv-miR-UL148D HL
hsa-miR-337-3p HL hsa-miR-9*/mmu- HL miR-9*/rno-miR-9*
hsa-miR-200b*/mmu- HL miR-200b* ebv-miR-BART9* HL hsa-miR-342- HL
5p/mmu-miR-342- 5p/rno-miR-342-5p hsa-miR-206/mmu- HL
miR-206/rno-miR- 206 hcmv-miR-US25-1* HL hsa-miR-659 HL hsa-miR-514
HL kshv-miR-K12-6-5p HL hsa-miR-508-5p HL hsa-miR-377* HL
ebv-miR-BART16 HL hsa-miR-181b/mmu- HL miR-181b/rno-miR- 181b
hsa-miR-622 HL kshv-miR-K12-1 HL hsa-miR-490- HL 3p/mmu-miR-490
hsa-miR-125b- HL 1*/mmu-miR-125b- 3p/rno-miR-125b-3p
hsa-miR-124/mmu- HL miR-124/rno-miR- 124 hsa-miR-657 HL
ebv-miR-BHRF1-3 HL kshv-miR-K12-5 HL hsa-miR-487b/mmu- HL
miR-487b/rno-miR- 487b hsa-miR-183*/mmu- HL miR-183*
hsa-miR-297/mmu- HL miR-297a hsa-miR-885-5p HL hsa-miR-296- HL
3p/mmu-miR-296- 3p/rno-miR-296 ebv-miR-BART19-3p HL hsa-miR-617 HL
hsa-miR-519d HL hsa-miR-195* HL hsa-miR-575 HL hsa-miR-208a/mmu- HL
miR-208a/rno-miR- 208 hsa-miR-647 HL hsa-miR-525-5p HL
ebv-miR-BART8* HL hsa-miR-340*/mmu- HL miR-340-3p/rno-miR- 340-3p
hsa-miR-220b HL hsa-miR-382/mmu- HL miR-382/rno-miR- 382
hsa-miR-585 HL hsa-miR-877/mmu- HL miR-877/rno-miR- 877
hsa-miR-99b*/mmu- HL miR-99b*/rno-miR- 99b* ebv-miR-BHRF1-1 HL
hsa-miR-326/mmu- HL miR-326/rno-miR- 326 ebv-miR-BART7* HL
hsa-miR-615- HL 3p/mmu-miR-615-3p mghv-miR-M1-8 HL hsa-miR-193b* HL
ebv-miR-BART13 HL hsa-miR-433/mmu- HL miR-433/rno-miR- 433
hsa-miR-202 HL hsa-miR-551b* HL hsa-miR-551a HL hsa-miR-542- HL
3p/mmu-miR-542- 3p/rno-miR-542-3p hsa-miR-338- HL 5p/mmu-miR-338-
5p/rno-miR-338*
hsa-miR-299-3p HL hsa-miR-518a-3p HL hsa-miR-181a-2* HL hsa-miR-938
HL hsa-miR-509-3-5p HL hsa-miR-552 HL
TABLE-US-00027 TABLE 21 Predictor microRNAs that distinguish
germinal center B-cell like (GCB) DLBCL from Burkitt lymphoma
GCBvsBL Higher in hsa-miR-129* GCB hsa-miR-28-5p/mmu-miR-28/rno-
GCB miR-28 hsa-miR-155 GCB hsa-miR-196a*/mmu-miR-196a*/rno- GCB
miR-196a* hsa-miR-146a/mmu-miR-146a/rno- GCB miR-146a
hsa-miR-331-3p/mmu-miR-331- GCB 3p/rno-miR-331 hsa-miR-215 GCB
hsa-miR-600 GCB mghv-miR-M1-7-3p GCB hsa-miR-107/mmu-miR-107/rno-
GCB miR-107 hsa-miR-886-3p GCB hsa-miR-140-5p/mmu-miR-140/rno- GCB
miR-140 hsa-miR-154/mmu-miR-154/rno- GCB miR-154
hsa-miR-103/mmu-miR-103/rno- GCB miR-103 hsa-let-7g/mmu-let-7g GCB
hsa-miR-222/mmu-miR-222/rno- GCB miR-222
hsa-miR-221/mmu-miR-221/rno- GCB miR-221
hsa-miR-320/mmu-miR-320/rno- GCB miR-320
hsa-miR-140-3p/mmu-miR-140*/rno- GCB miR-140*
hsa-miR-148a/mmu-miR-148a GCB hsa-miR-365/mmu-miR-365/rno-miR- GCB
365 hsa-miR-29c/mmu-miR-29c/rno-miR- GCB 29c
hsa-miR-30d/mmu-miR-30d/rno-miR- GCB 30d
hsa-miR-214/mmu-miR-214/rno-miR- GCB 214
hsa-miR-146b-5p/mmu-miR-146b/rno- GCB miR-146b
hsa-miR-342-3p/mmu-miR-342-3p/rno- GCB miR-342-3p hsa-miR-374a GCB
hsa-miR-223/mmu-miR-223/rno-miR- GCB 223
hsa-miR-29b/mmu-miR-29b/rno-miR- GCB 29b
hsa-miR-21/mmu-miR-21/rno-miR-21 GCB
hsa-miR-29a/mmu-miR-29a/rno-miR- GCB 29a
hsa-miR-23b/mmu-miR-23b/rno-miR- GCB 23b
hsa-miR-24/mmu-miR-24/rno-miR-24 GCB
hsa-miR-26a/mmu-miR-26a/rno-miR- GCB 26a
hsa-miR-26b/mmu-miR-26b/rno-miR- GCB 26b hsa-miR-34b/mmu-miR-34b-3p
GCB hsa-miR-503 BL hsa-miR-30b* BL
TABLE-US-00028 TABLE 22 Predictor microRNAs that distinguish
germinal center B-cell like (GCB) DLBCL from chronic lymphocytic
leukemia Higher GCB vs CLL in hsa-miR-181a/mmu- GCB
miR-181a/rno-miR- 181a hsa-miR-886-5p GCB mghv-miR-M1-7-3p GCB
hsa-miR-934 GCB mghv-miR-M1-3 GCB hsa-miR-485- GCB 3p/mmu-miR-485*
hsa-miR-125b/mmu- GCB miR-125b-5p/rno- miR-125b-5p hsa-miR-637 GCB
hsa-miR-365/mmu- GCB miR-365/rno-miR- 365 hsa-miR-505* GCB
hsa-miR-199a- GCB 3p/hsa-miR-199b- 3p/mmu-miR-199a- 3p/mmu-miR-
199b/rno-miR-199a- 3p hsa-miR-675 GCB hsa-miR-424 GCB
ebv-miR-BHRF1-2 GCB hsa-miR-519c- GCB 5p/hsa-miR-519b- 5p/hsa-miR-
523*/hsa-miR- 518e*/hsa-miR- 522*/hsa-miR- 519a* hsa-miR-130a/mmu-
GCB miR-130a/rno-miR- 130a hsa-miR-943 GCB hsa-miR-126/mmu- GCB
miR-126-3p/rno- miR-126 hsa-miR-193b GCB hsa-miR-198 GCB hsa-miR-
GCB 200b*/mmu-miR- 200b* kshv-miR-K12-6-3p GCB hsa-miR-220c GCB
hsa-miR-374b* GCB hsa-miR-518b GCB hsa-miR-920 GCB hsa-miR-125b-
GCB 1*/mmu-miR-125b- 3p/rno-miR-125b-3p ebv-miR-BART8* GCB
ebv-miR-BART16 GCB hsa-miR-630 GCB hsa-miR-483-5p GCB hsa-miR-422a
GCB hsa-miR-526b GCB hsa-miR-145/mmu- GCB miR-145/rno-miR- 145
hsa-miR-126*/mmu- GCB miR-126-5p/rno- miR-126* hsa-miR-143/mmu- GCB
miR-143/rno-miR- 143 hsa-miR-100/mmu- GCB miR-100/rno-miR-100
hsa-miR-371-5p GCB hsa-miR-193a-5p GCB hsa-miR-628-3p GCB
hsa-miR-185* GCB hsa-miR-10b/mmu- GCB miR-10b/rno-miR-10b
hsa-miR-665 GCB hsa-miR-503 GCB hsa-miR-642 GCB hsa-miR-658 GCB
hsa-miR-21/mmu- GCB miR-21/rno-miR-21 hsa-miR-23a/mmu- GCB
miR-23a/rno-miR-23a hsa-miR-125a- GCB 5p/mmu-miR-125a-
5p/rno-miR-125a-5p hsa-miR-24/mmu- GCB miR-24/rno-miR-24
hsa-miR-23b/mmu- GCB miR-23b/rno-miR-23b hsa-miR-620 GCB
hsa-miR-933 GCB hsa-miR-30b* GCB hsa-miR-22/mmu- GCB
miR-22/rno-miR-22 hsa-let-7e/mmu-let- GCB 7e/rno-let-7e
mghv-miR-M1-4 GCB hsa-miR-149* GCB hsa-miR-765 GCB
hsa-let-7c/mmu-let- GCB 7c/rno-let-7c hsa-miR-423- CLL
5p/mmu-miR-423-5p hsa-miR-32* CLL hsa-miR-34b/mmu- CLL miR-34b-3p
hsa-miR-551b/mmu- CLL miR-551b/rno-miR- 551b hsa-let-7i/mmu-let-
CLL 7i/rno-let-7i hsa-miR-29a/mmu- CLL miR-29a/rno-miR-29a
hsa-miR-138- CLL 1*/mmu-miR- 138*/rno-miR-138* hsa-miR-29b/mmu- CLL
miR-29b/rno-miR-29b hsa-miR-191/mmu- CLL miR-191/rno-miR-191
hsa-miR-26b/mmu- CLL miR-26b/rno-miR-26b hsa-miR-26a/mmu- CLL
miR-26a/rno-miR-26a hsa-miR-550 CLL hsa-miR-374a CLL
hsa-miR-142-5p/mmu- CLL miR-142-5p/rno-miR- 142-5p hsa-miR-29c/mmu-
CLL miR-29c/rno-miR-29c hsa-miR-140-3p/mmu- CLL miR-140*/rno-miR-
140* hsa-miR-30e/mmu- CLL miR-30e/rno-miR-30e hsa-miR-801/mmu- CLL
miR-801 hsa-miR-768-3p CLL hsa-miR-549 CLL hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR- 199a-5p hsa-miR-223/mmu- CLL
miR-223/rno-miR-223 hsa-miR-101/mmu- CLL miR-101a/rno-miR- 101a
hsa-miR-888* CLL hsa-miR-24-1*/mmu- CLL miR-24-1*/rno-miR-24- 1*
hsa-miR-519d CLL hsa-miR-154/mmu- CLL miR-154/rno-miR-154
hsa-miR-638 CLL hsa-miR-668/mmu- CLL miR-668 hsa-miR-891a CLL
hsa-miR-768-5p CLL hsa-miR-140-5p/mmu- CLL miR-140/rno-miR-140
hsa-miR-196a*/mmu- CLL miR-196a*/rno-miR- 196a* hsa-miR-150/mmu-
CLL miR-150/rno-miR-150 hsa-let-7g/mmu-let-7g CLL hsa-miR-363/mmu-
CLL miR-363/rno-miR-363 hsa-miR-486-5p/mmu- CLL miR-486
hsa-miR-32/mmu-miR- CLL 32/rno-miR-32 hsa-miR-147 CLL hsa-miR-20b*
CLL hsa-miR-487b/mmu- CLL miR-487b/rno-miR- 487b hsa-miR-636 CLL
hsa-miR-144* CLL hsa-miR-186/mmu- CLL miR-186/rno-miR-186
hsa-miR-30e*/mmu- CLL miR-30e*/rno-miR- 30e* hsa-miR-331-5p/mmu-
CLL miR-331-5p
TABLE-US-00029 TABLE 23 Predictor microRNAs that distinguish
germinal center B-cell like (GCB) DLBCL from follicular lymphoma
Higher GCB vs FL in hsa-miR-378/mmu- GCB miR-378/rno-miR-378
hsa-miR-20b/mmu- GCB miR-20b/rno-miR-20b- 5p hsa-miR-19b/mmu- GCB
miR-19b/rno-miR-19b hsa-miR-106a GCB hsa-miR-17/mmu-miR- GCB
17/rno-miR-17-5p/rno- miR-17 hsa-miR-93/mmu-miR- GCB 93/rno-miR-93
hsa-miR-20a/mmu- GCB miR-20a/rno-miR-20a hsa-miR-23b/mmu- GCB
miR-23b/rno-miR-23b hsa-miR-23a/mmu- GCB miR-23a/rno-miR-23a
hsa-miR-22/mmu-miR- GCB 22/rno-miR-22 hsa-miR-19a/mmu- GCB
miR-19a/rno-miR-19a hsa-miR-320/mmu- GCB miR-320/rno-miR-320
hsa-miR-106b/mmu- GCB miR-106b/rno-miR- 106b hsa-miR-103/mmu- GCB
miR-103/rno-miR-103 hsa-miR-30c/mmu- GCB miR-30c/rno-miR-30c
ebv-miR-BHRF1-2 GCB hsa-miR-125a- GCB 5p/mmu-miR-125a-
5p/rno-miR-125a-5p hsa-let-7a/mmu-let- GCB 7a/rno-let-7a
hsa-miR-628-3p GCB hsa-let-7c/mmu-let- GCB 7c/rno-let-7c
hsa-miR-423-5p/mmu- FL miR-423-5p hsa-miR-24-1*/mmu- FL
miR-24-1*/rno-miR- 24-1* ebv-miR-BART2-3p FL hsa-miR-138-1*/mmu- FL
miR-138*/rno-miR- 138* hsa-miR-768-5p FL hsa-miR-30b* FL
hsa-miR-494/mmu- FL miR-494/rno-miR-494 hsa-miR-583 FL
hsa-miR-185/mmu- FL miR-185/rno-miR-185 hsa-miR-765 FL
hsa-miR-34b/mmu- FL miR-34b-3p hsa-miR-921 FL hsa-miR-551b/mmu- FL
miR-551b/rno-miR- 551b hsa-miR-549 FL hsa-miR-939 FL hsa-miR-302d*
FL ebv-miR-BART17-5p FL hsa-miR-801/mmu- FL miR-801 hsa-miR-888* FL
hsa-miR-620 FL hsa-miR-576-3p FL hsa-miR-32* FL hsa-miR-574-5p/mmu-
FL miR-574-5p hsa-miR-505/rno-miR- FL 505 hsa-miR-885-5p FL
hsa-miR-455-3p FL hsa-miR-152/mmu- FL miR-152/rno-miR-152
hsa-miR-200b*/mmu- FL miR-200b* mghv-miR-M1-2 FL hcmv-miR-UL148D FL
hsa-miR-497/mmu-miR- FL 497/rno-miR-497 ebv-miR-BART13 FL
ebv-miR-BART16 FL hsa-miR-125b-2*/rno- FL miR-125b*
hsa-miR-490-3p/mmu- FL miR-490 hsa-miR-99b/mmu-miR- FL
99b/rno-miR-99b hsa-miR-339-5p/mmu- FL miR-339-5p/rno-miR- 339-5p
hsa-miR-574-3p/mmu- FL miR-574-3p hsa-miR-515-5p FL
hsa-miR-877/mmu-miR- FL 877/rno-miR-877 hsa-miR-208a/mmu-miR- FL
208a/rno-miR-208 hcmv-miR-US25-1* FL hsa-miR-326/mmu-miR- FL
326/rno-miR-326 hsa-miR-488 FL hsa-miR-629 FL hsa-miR-24-2*/mmu- FL
miR-24-2*/rno-miR-24- 2* hsa-miR-124/mmu-miR- FL 124/rno-miR-124
hsa-miR-493 FL hsv1-miR-H1 FL hsa-miR-484/mmu-miR- FL
484/rno-miR-484 hsa-miR-483-3p FL hsa-miR-27a*/mmu-miR- FL
27a*/rno-miR-27a* hsa-miR-144* FL hsa-miR-617 FL hsa-miR-377* FL
hsa-miR-363*/rno-miR- FL 363* hsa-miR-148b/mmu-miR- FL
148b/rno-miR-148b-3p kshv-miR-K12-1 FL hsa-miR-7/mmu-miR- FL
7a/rno-miR-7a hsa-miR-193b* FL hsa-miR-542-3p/mmu- FL
miR-542-3p/rno-miR- 542-3p hsa-miR-601 FL hsa-miR-106b*/mmu- FL
miR-106b*/rno-miR- FL 106b* hsa-miR-7-2* FL kshv-miR-K12-6-5p FL
hsa-miR-645 FL hsa-miR-524-5p FL hsa-miR-548d-5p FL
hsa-miR-9*/mmu-miR- FL 9*/rno-miR-9* hsa-miR-92a/mmu-miR- FL
92a/rno-miR-92a hsa-miR-22*/mmu-miR- FL 22*/rno-miR-22*
hsa-miR-500* FL hsa-miR-890 FL hsa-miR-297/mmu-miR- FL 297a
hsa-miR-197/mmu-miR- FL 197 hsa-miR-20b* FL hsa-miR-629* FL
hsa-miR-887 FL hsa-miR-425/mmu- FL miR-425/rno-miR-425
hsa-miR-99b*/mmu- FL miR-99b*/rno-miR- 99b* hsa-miR-513a-3p FL
hsa-miR-206/mmu- FL miR-206/rno-miR-206 hsa-miR-155* FL
hsa-miR-181b/mmu- FL miR-181b/rno-miR- 181b hsa-miR-299-3p FL
hsa-miR-218-2*/mmu- FL miR-218-2*/rno-miR- 218* mghv-miR-M1-8 FL
ebv-miR-BART18-3p FL hsa-miR-708/mmu- FL miR-708/rno-miR-708
hsa-miR-409-5p/mmu- FL miR-409-5p/rno-miR- 409-5p hsa-miR-553 FL
hsa-miR-361-3p FL hsa-miR-296-3p/mmu- FL miR-296-3p/rno-miR- 296
ebv-miR-BHRF1-3 FL hsa-miR-34c-5p/mmu- FL miR-34c/rno-miR-34c
hsa-let-7b*/mmu-let- FL 7b*/rno-let-7b* hsa-miR-301a/mmu- FL
miR-301a/rno-miR- 301a hsa-miR-122* FL hsa-miR-183/mmu- FL
miR-183/rno-miR-183 kshv-miR-K12-5 FL kshv-miR-K12-7 FL hsa-miR-552
FL hsa-miR-151-3p FL hsa-miR-194/mmu- FL miR-194/rno-miR-194
hsa-miR-585 FL hsa-miR-340*/mmu- FL miR-340-3p/rno-miR- 340-3p
hsa-let-7d*/mmu-let- FL 7d*/rno-let-7d* hsa-miR-622 FL hsa-miR-575
FL hsa-miR-514 FL hsa-miR-92b/mmu- FL miR-92b/rno-miR-92b
hsa-miR-551a FL hsa-miR-221* FL hsa-miR-922 FL hsa-miR-938 FL
hsa-miR-615-3p/mmu- FL miR-615-3p hsa-miR-220b FL hsa-miR-744/mmu-
FL miR-744 hsa-miR-657 FL hsa-miR-382/mmu- FL miR-382/rno-miR-382
hsa-miR-518a-3p FL hsa-miR-138/mmu- FL miR-138/rno-miR-138
hsa-miR-636 FL hsa-miR-96/mmu- FL miR-96/rno-miR-96
hsa-miR-509-3-5p FL hsa-miR-337-3p FL hsa-miR-342-5p/mmu- FL
miR-342-5p/rno-miR- 342-5p
TABLE-US-00030 TABLE 24 Predictor microRNAs that distinguish
germinal center B-cell like (GCB) DLBCL from Hodgkin's lymphoma
Higher GCB vs HL in hsa-miR-19b/mmu-miR- GCB 19b/rno-miR-19b
hsa-miR-19a/mmu-miR- GCB 19a/rno-miR-19a hsa-miR-106a GCB
hsa-miR-20b/mmu-miR- GCB 20b/rno-miR-20b-5p hsa-miR-17/mmu-miR- GCB
17/rno-miR-17-5p/rno- miR-17 hsa-miR-15b/mmu-miR- GCB
15b/rno-miR-15b hsa-miR-20a/mmu-miR- GCB 20a/rno-miR-20a
hsa-miR-30b/mmu-miR- GCB 30b/rno-miR-30b-5p hsa-miR-142-3p/mmu-miR-
GCB 142-3p/rno-miR-142-3p hsa-miR-30c/mmu-miR- GCB 30c/rno-miR-30c
hsa-miR-378/mmu-miR- GCB 378/rno-miR-378 hsa-miR-93/mmu-miR- GCB
93/rno-miR-93 hsa-miR-106b/mmu-miR- GCB 106b/rno-miR-106b
hsa-miR-374a GCB hsa-miR-24/mmu-miR- GCB 24/rno-miR-24
hsa-miR-29b/mmu-miR- GCB 29b/rno-miR-29b hsa-miR-22/mmu-miR- GCB
22/rno-miR-22 hsa-miR-142-5p/mmu-miR- GCB 142-5p/rno-miR-142-5p
hsa-miR-30e/mmu-miR- GCB 30e/rno-miR-30e hsa-miR-30a/mmu-miR- GCB
30a/rno-miR-30a hsa-miR-30d/mmu-miR- GCB 30d/rno-miR-30d
hsa-miR-23b/mmu-miR- GCB 23b/rno-miR-23b hsa-miR-16/mmu-miR- GCB
16/rno-miR-16 hsa-miR-191/mmu-miR- GCB 191/rno-miR-191
hsa-miR-15a/mmu-miR- GCB 15a hsa-miR-26b/mmu-miR- GCB
26b/rno-miR-26b hsa-miR-23a/mmu-miR- GCB 23a/rno-miR-23a
hsa-let-7a/mmu-let-7a/rno- GCB let-7a hsa-miR-103/mmu-miR- GCB
103/rno-miR-103 hsa-miR-140-3p/mmu-miR- GCB 140*/rno-miR-140*
hsa-miR-154/mmu-miR- GCB 154/rno-miR-154 hsa-miR-320/mmu-miR- GCB
320/rno-miR-320 hsa-miR-550 GCB hsa-miR-125a-5p/mmu- GCB
miR-125a-5p/rno-miR- 125a-5p hsa-let-7c/mmu-let-7c/rno- GCB let-7c
hsa-miR-185/mmu-miR- HL 185/rno-miR-185 hsa-miR-658 HL hsa-miR-549
HL hsa-miR-634 HL hsa-miR-551b/mmu-miR- HL 551b/rno-miR-551b
hsa-miR-518c* HL hsa-miR-888* HL hsa-miR-765 HL
hsa-miR-423-5p/mmu-miR- HL 423-5p hsa-miR-30c-2*/mmu-miR- HL
30c-2*/rno-miR-30c-2* hsa-miR-503 HL hsa-miR-921 HL hsa-miR-520d-5p
HL hsa-miR-574-5p/mmu-miR- HL 574-5p hsa-miR-32* HL hsa-miR-939 HL
ebv-miR-BART2-3p HL ebv-miR-BHRF1-2 HL hsa-miR-583 HL hsa-miR-30b*
HL hsa-miR-149* HL mghv-miR-M1-4 HL hsa-miR-513a-5p HL
hsa-miR-494/mmu-miR- HL 494/rno-miR-494 hsa-miR-498 HL
hsa-miR-485-3p/mmu-miR- HL 485* hsa-miR-129-5p/mmu-miR- HL
129-5p/rno-miR-129 hsa-miR-25* HL hsa-miR-923 HL hsa-miR-519d HL
hsa-miR-516a-5p HL hsa-miR-99a/mmu-miR- HL 99a/rno-miR-99a
hsa-miR-943 HL hsa-miR-885-5p HL ebv-miR-BHRF1-1 HL
hsa-miR-152/mmu-miR- HL 152/rno-miR-152 ebv-miR-BART8* HL
hsa-miR-200b*/mmu-miR- HL 200b* hsa-miR-125b-1*/mmu- HL
miR-125b-3p/rno-miR- 125b-3p hsa-miR-526b HL hsa-miR-29a*/mmu-miR-
HL 29a*/rno-miR-29a* hsa-miR-532-5p/mmu-miR- HL
532-5p/rno-miR-532-5p hsa-miR-183*/mmu-miR- HL 183* hsa-miR-659 HL
hsa-miR-145*/mmu-miR- HL 145* mghv-miR-M1-7-5p HL hsa-miR-143* HL
hsa-miR-660 HL hsa-miR-130b/mmu- HL miR-130b/rno-miR-130b
hsa-miR-671-5p/mmu- HL miR-671-5p hsa-miR-525-5p HL
hsa-miR-505/rno-miR- HL 505 hsa-miR-488 HL hsa-miR-766 HL
hsa-miR-20b* HL hsa-miR-339-5p/mmu- HL miR-339-5p/rno-miR- 339-5p
hsa-miR-524-5p HL hsa-miR-455-3p HL hsa-miR-92a/mmu-miR- HL
92a/rno-miR-92a hsa-miR-502-3p HL hsa-miR-210/mmu-miR- HL
210/rno-miR-210 hsv1-miR-H1 HL ebv-miR-BART6-3p HL
hsa-miR-490-3p/mmu- HL miR-490 hsa-miR-149/mmu-miR- HL 149
hsa-miR-128/mmu-miR- HL 128/rno-miR-128 hsa-miR-635 HL
hcmv-miR-UL148D HL hsa-miR-373* HL hsa-miR-647 HL
hsa-miR-197/mmu-miR- HL 197 hsa-miR-602 HL hsa-miR-656 HL
hsa-miR-874/mmu-miR- HL 874/rno-miR-874 ebv-miR-BART19-3p HL
hsa-miR-551b* HL hsa-miR-96/mmu-miR- HL 96/rno-miR-96 hsa-miR-889
HL hsa-miR-425/mmu-miR- HL 425/rno-miR-425 hsa-miR-34c-5p/mmu- HL
miR-34c/rno-miR-34c hcmv-miR-UL70-3p HL hsa-miR-27a*/mmu- HL
miR-27a*/rno-miR-27a* hsa-miR-194/mmu-miR- HL 194/rno-miR-194
hsa-miR-17*/rno-miR- HL 17-3p hsa-miR-548d-5p HL hsa-miR-7/mmu-miR-
HL 7a/rno-miR-7a hsa-miR-877/mmu-miR- HL 877/rno-miR-877
hsa-miR-22*/mmu-miR- HL 22*/rno-miR-22* hsa-miR-323-3p/mmu- HL
miR-323-3p/rno-miR- 323 hsa-miR-708/mmu-miR- HL 708/rno-miR-708
hsa-miR-513a-3p HL hsa-miR-595 HL hsa-miR-922 HL hsa-miR-515-5p HL
hsa-miR-99b/mmu-miR- HL 99b/rno-miR-99b hsa-miR-483-3p HL
hsa-miR-330-5p/mmu- HL miR-330/rno-miR-330 hsa-miR-509-3p HL
hsa-miR-151-3p HL ebv-miR-BART13 HL hsa-miR-617 HL
hsa-miR-328/mmu-miR- HL 328/rno-miR-328 hsa-miR-361-3p HL
hsa-miR-138/mmu-miR- HL 138/rno-miR-138 ebv-miR-BART7* HL
hsa-miR-589 HL hsa-miR-576-5p HL hsa-miR-452 HL hsa-miR-7-2* HL
hsa-miR-296-5p/mmu- HL miR-296-5p/rno-miR- 296* hsa-miR-550* HL
hsa-miR-92b/mmu-miR- HL 92b/rno-miR-92b kshv-miR-K12-1 HL
hsa-miR-526a/hsa-miR- HL 520c-5p/hsa-miR-518d- 5p mghv-miR-M1-2 HL
hsa-miR-548b-3p HL hsa-miR-297/mmu-miR- HL 297a hsa-miR-195* HL
hsa-miR-652/mmu-miR- HL 652/rno-miR-652 hsa-miR-221* HL
hsa-miR-194* HL hsa-miR-23a*/rno-miR- HL
23a* hsa-miR-125b-2*/rno- HL miR-125b* hsa-miR-212/mmu-miR- HL
212/rno-miR-212 ebv-miR-BART18-3p HL hsa-miR-586 HL
hsa-miR-137/mmu-miR- HL 137/rno-miR-137 hsa-miR-610 HL
hcmv-miR-UL112 HL hsa-miR-181b/mmu- HL miR-181b/rno-miR-181b
hsa-miR-936 HL hsa-miR-744/mmu- HL miR-744 kshv-miR-K12-6-5p HL
hcmv-miR-US25-1* HL hsa-miR-21* HL mghv-miR-M1-6 HL mghv-miR-M1-8
HL hsa-miR-10a*/mmu- HL miR-10a*/rno-miR- 10a-3p hsa-miR-345 HL
hsa-miR-887 HL hsa-miR-193b* HL hsa-miR-122* HL kshv-miR-K12-5 HL
hsa-miR-92b* HL hsa-miR-526b* HL hsa-miR-553 HL hsa-miR-601 HL
hiv1-miR-H1 HL hsa-miR-623 HL hsa-miR-519e HL hsa-miR-650 HL
hsa-miR-575 HL hsa-miR-629* HL hsa-miR-890 HL hsa-miR- HL
150*/mmu-miR- 150* hsa-miR- HL 425*/mmu-miR- 425* hsa-miR- HL
135a*/mmu-miR- 135a* hsa-miR-612 HL hsa-miR-636 HL hsa-miR-500 HL
hsa-miR-502-5p HL hsa-miR-9*/mmu- HL miR-9*/rno-miR-9* hsa-miR-500*
HL hsa-miR- HL 124*/mmu-miR- 124*/rno-miR-124* hsa-miR-30c- HL
1*/mmu-miR-30c- 1*/rno-miR-30c-1* hsa-miR- HL 99b*/mmu-miR-
99b*/rno-miR-99b* hsa-miR-331- HL 5p/mmu-miR-331- 5p
hsa-miR-206/mmu- HL miR-206/rno-miR- 206 hsa-miR-376a* HL
hsa-miR-585 HL hsa-miR-640 HL hsa-miR-377* HL hsa-miR-125a- HL
3p/mmu-miR-125a- 3p/rno-miR-125a-3p hsa-miR-24- HL 2*/mmu-miR-24-
2*/rno-miR-24-2* hsa-miR-484/mmu- HL miR-484/rno-miR- 484 hsa-miR-
HL 106b*/mmu-miR- 106b*/rno-miR- 106b* hsa-let-7b*/mmu- HL
let-7b*/rno-let-7b* hsa-miR-302c* HL hsa-miR-542- HL
5p/mmu-miR-542- 5p/rno-miR-542-5p hsa-miR-622 HL ebv-miR-BHRF1-3 HL
hsa-miR-181a-2* HL hsa-miR-183/mmu- HL miR-183/rno-miR- 183
hsa-miR-409- HL 5p/mmu-miR-409- 5p/rno-miR-409-5p ebv-miR-BART20-
HL 3p hsa-miR-629 HL hsa-let-7d*/mmu- HL let-7d*/rno-let-7d*
hsa-miR-645 HL hsa-miR-492 HL hsa-miR-654-5p HL hsa-miR-208a/mmu-
HL miR-208a/rno-miR- 208 hsa-miR-584 HL hsa-miR-382/mmu- HL
miR-382/rno-miR- 382 hsa-miR- HL 340*/mmu-miR- 340-3p/rno-miR-
340-3p hsa-miR-490-5p HL hsa-miR-663 HL hsa-miR-337-3p HL
hsa-miR-518a-3p HL ebv-miR-BART9* HL hsa-miR-342- HL
5p/mmu-miR-342- 5p/rno-miR-342-5p hsa-miR-514 HL hsa-miR-508-5p HL
hsa-miR-124/mmu- HL miR-124/rno-miR- 124 hsa-miR-657 HL hsa-miR-938
HL hsa-miR-296- HL 3p/mmu-miR-296- 3p/rno-miR-296 hsa-miR-551a HL
hsa-miR-542- HL 3p/mmu-miR-542- 3p/rno-miR-542-3p hsa-miR-220b HL
hsa-miR-326/mmu- HL miR-326/rno-miR- 326 hsa-miR-615- HL
3p/mmu-miR-615- 3p hsa-miR-433/mmu- HL miR-433/rno-miR- 433
hsa-miR-202 HL hsa-miR-338- HL 5p/mmu-miR-338- 5p/rno-miR-338*
hsa-miR-552 HL hsa-miR-299-3p HL hsa-miR-509-3-5p HL
TABLE-US-00031 TABLE 25 Predictor microRNAs that distinguish
Burkitt lymphoma from chromic lymphocytic leukemia Higher BL vs CLL
in hsa-miR-874/mmu- BL miR-874/rno-miR-874 hsa-miR-125b/mmu- BL
miR-125b-5p/rno-miR- 125b-5p hsa-miR-126/mmu- BL
miR-126-3p/rno-miR- 126 ebv-miR-BHRF1-2 BL hsa-miR-193b BL
hsa-miR-371-5p BL hsa-miR-193a-5p BL hsa-miR-628-3p BL hsa-miR-185*
BL hsa-miR-503 BL hsa-miR-199a-3p/hsa- BL miR-199b-3p/mmu-
miR-199a-3p/mmu- miR-199b/rno-miR- 199a-3p hsa-miR-143/mmu- BL
miR-143/rno-miR-143 hsa-miR-130a/mmu- BL miR-130a/rno-miR- 130a
hsa-miR-145/mmu- BL miR-145/rno-miR-145 hsa-miR-30b* BL hsa-miR-665
BL hsa-miR-658 BL hsa-miR-933 BL hsa-miR-30c-2*/mmu- BL
miR-30c-2*/rno-miR- 30c-2* hsa-miR-765 BL hsa-miR-620 BL
hsa-miR-520d-5p BL hsa-miR-494/mmu- BL miR-494/rno-miR-494
hsa-miR-551b/mmu- CLL miR-551b/rno-miR- 551b hsa-miR-106b/mmu- CLL
miR-106b/rno-miR- 106b hsa-miR-30c/mmu- CLL miR-30c/rno-miR-30c
hsa-miR-16/mmu-miR- CLL 16/rno-miR-16 hsa-miR-27a/mmu- CLL
miR-27a/rno-miR-27a hsa-miR-27b/mmu- CLL miR-27b/rno-miR-27b
hsa-miR-550 CLL hsa-miR-30a/mmu- CLL miR-30a/rno-miR-30a
hsa-miR-30b/mmu-miR- CLL 30b/rno-miR-30b-5p hsa-miR-34b/mmu-miR-
CLL 34b-3p hsa-miR-801/mmu-miR- CLL 801 hsa-miR-26b/mmu-miR- CLL
26b/rno-miR-26b hsa-let-7b/mmu-let- CLL 7b/rno-let-7b
hsa-miR-142-5p/mmu- CLL miR-142-5p/rno-miR- 142-5p
hsa-miR-26a/mmu-miR- CLL 26a/rno-miR-26a hsa-miR-29a/mmu-miR- CLL
29a/rno-miR-29a hsa-miR-768-3p CLL hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR- 199a-5p hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e hsa-miR-29b/mmu-miR- CLL 29b/rno-miR-29b
hsa-miR-101/mmu-miR- CLL 101a/rno-miR-101a hsa-miR-138-1*/mmu- CLL
miR-138*/rno-miR-138* hsa-miR-195/mmu-miR- CLL 195/rno-miR-195
hsa-miR-549 CLL hsa-miR-103/mmu-miR- CLL 103/rno-miR-103
hsa-miR-649 CLL hsa-miR-335/mmu-miR- CLL 335-5p/rno-miR-335
hsa-miR-342-3p/mmu- CLL miR-342-3p/rno-miR- 342-3p
hsa-miR-423-3p/mmu- CLL miR-423-3p/rno-miR- 423
hsa-miR-222/mmu-miR- CLL 222/rno-miR-222 hsa-miR-374a CLL
hsa-miR-888* CLL hsa-miR-30d/mmu-miR- CLL 30d/rno-miR-30d
hsa-miR-299-5p/mmu- CLL miR-299*/rno-miR-299 hsa-miR-107/mmu-miR-
CLL 107/rno-miR-107 hsa-miR-105 CLL hsa-let-7f/mmu-let- CLL
7f/rno-let-7f hsa-miR-191/mmu-miR- CLL 191/rno-miR-191
hsa-miR-223/mmu-miR- CLL 223/rno-miR-223 hsa-miR-361-5p/mmu- CLL
miR-361/rno-miR-361 hsa-miR-29c/mmu- CLL miR-29c/rno-miR-29c
hsa-miR-147 CLL hsa-miR-361-3p CLL hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR- 140* hsa-miR-486-5p/mmu- CLL miR-486
hsa-miR-33a/mmu- CLL miR-33/rno-miR-33 hsa-miR-636 CLL
hsa-miR-24-1*/mmu- CLL miR-24-1*/rno-miR-24- 1* hsa-miR-144* CLL
hsa-miR-668/mmu- CLL miR-668 hsa-miR-768-5p CLL hsa-miR-363/mmu-
CLL miR-363/rno-miR-363 hsa-miR-150/mmu- CLL miR-150/rno-miR-150
hsa-miR-519d CLL hsa-miR-891a CLL hsa-miR-186/mmu- CLL
miR-186/rno-miR-186 hsa-miR-331-5p/mmu- CLL miR-331-5p
hsa-miR-28-5p/mmu- CLL miR-28/rno-miR-28 hsa-miR-154/mmu- CLL
miR-154/rno-miR-154 hsa-miR-155 CLL hsa-miR-363*/rno-miR- CLL 363*
hsa-miR-32/mmu-miR- CLL 32/rno-miR-32 hsa-miR-30e*/mmu- CLL
miR-30e*/rno-miR- 30e* hsa-miR-140-5p/mmu- CLL miR-140/rno-miR-140
hsa-let-7g/mmu-let-7g CLL hsa-miR-20b* CLL hsa-miR-129* CLL
hsa-miR-196a*/mmu- CLL miR-196a*/rno-miR- 196a* hsa-miR-487b/mmu-
CLL miR-487b/rno-miR- 487b
TABLE-US-00032 TABLE 26 Predictor microRNAs that distinguish
Burkitt lymphoma from follicular lymphoma Higher BL vs FL in
hsa-miR-17/mmu-miR- BL 17/rno-miR-17-5p/rno- miR-17 hsa-miR-106a BL
hsa-miR-19b/mmu-miR- BL 19b/rno-miR-19b hsa-miR-20a/mmu-miR- BL
20a/rno-miR-20a hsa-miR-19a/mmu-miR- BL 19a/rno-miR-19a
hsa-miR-628-3p BL hsa-miR-503 BL hsa-miR-371-5p BL
hsa-miR-106b/mmu- BL miR-106b/rno-miR-106b hsa-miR-30c-2*/mmu- BL
miR-30c-2*/rno-miR- 30c-2* ebv-miR-BART2-3p FL hsa-let-7e/mmu-let-
FL 7e/rno-let-7e hsa-miR-551b/mmu- FL miR-551b/rno-miR-551b
hsa-miR-26b/mmu-miR- FL 26b/rno-miR-26b hsa-miR-26a/mmu-miR- FL
26a/rno-miR-26a hsa-miR-620 FL hsa-miR-801/mmu-miR- FL 801
ebv-miR-BART17-5p FL hsa-miR-29a/mmu-miR- FL 29a/rno-miR-29a
hsa-miR-34b/mmu-miR- FL 34b-3p hsa-miR-32* FL hsa-miR-29b/mmu-miR-
FL 29b/rno-miR-29b hsa-miR-649 FL hsa-miR-576-3p FL
hsa-miR-302a/mmu- FL miR-302a hsa-miR-365/mmu-miR- FL
365/rno-miR-365 hsa-miR-148a/mmu- FL miR-148a hsa-miR-146b-5p/mmu-
FL miR-146b/rno-miR-146b hsa-miR-505/rno-miR- FL 505
hsa-miR-33a/mmu-miR- FL 33/rno-miR-33 hsa-miR-455-3p FL
hsa-miR-374b/mmu- FL miR-374/rno-miR-374 hsa-miR-214/mmu-miR- FL
214/rno-miR-214 hsa-miR-138-1*/mmu- FL miR-138*/rno-miR-138*
hsa-miR-140-3p/mmu- FL miR-140*/rno-miR-140* hsa-miR-212/mmu-miR-
FL 212/rno-miR-212 hsa-miR-29c/mmu-miR- FL 29c/rno-miR-29c
hsa-miR-888* FL hsa-miR-222/mmu-miR- FL 222/rno-miR-222
hsa-miR-152/mmu-miR- FL 152/rno-miR-152 hsa-miR-183*/mmu- FL
miR-183* hsa-miR-768-5p FL hsa-miR-107/mmu-miR- FL 107/rno-miR-107
hsa-miR-574-5p/mmu- FL miR-574-5p hsa-miR-154/mmu-miR- FL
154/rno-miR-154 hsa-miR-620 FL hsa-miR-886-5p FL hsa-miR-208a/mmu-
FL miR-208a/rno-miR-208 hsa-miR-374b* FL hsa-miR-525-5p FL
hsa-miR-363/mmu-miR- FL 363/rno-miR-363 hsa-miR-99b/mmu-miR- FL
99b/rno-miR-99b hsa-miR-148b/mmu- FL miR-148b/rno-miR- 148b-3p
kshv-miR-K12-6-5p FL hsa-miR-125b-1*/mmu- FL miR-125b-3p/rno-miR-
125b-3p hsa-miR-526b FL hsa-miR-629 FL hsa-miR-617 FL
hsa-miR-124/mmu-miR- FL 124/rno-miR-124 hsa-miR-493 FL
hsa-miR-24-1*/mmu- FL miR-24-1*/rno-miR- 24-1* hsa-miR-200b*/mmu-
FL miR-200b* hsa-miR-484/mmu- FL miR-484/rno-miR-484 hsa-miR-483-3p
FL hsa-miR-516b FL hsa-miR-125b-2*/rno- FL miR-125b*
hsa-miR-490-3p/mmu- FL miR-490 hsa-miR-140-5p/mmu- FL
miR-140/rno-miR-140 hsa-miR-877/mmu- FL miR-877/rno-miR-877
hsa-miR-381/mmu- FL miR-381/rno-miR-381 hsa-miR-193b* FL
hsa-miR-635 FL hsa-miR-542-3p/mmu- FL miR-542-3p/rno-miR- 542-3p
hsa-miR-181a-2* FL hsa-miR-32/mmu- FL miR-32/rno-miR-32 hsa-miR-105
FL hsa-miR-488 FL hsa-miR-505* FL ebv-miR-BART16 FL hsa-miR-891a FL
hsa-miR-221/mmu- FL miR-221/rno-miR-221 hsa-miR-7/mmu-miR- FL
7a/rno-miR-7a hsa-miR-299-3p FL hsa-miR-575 FL hsa-miR-585 FL
hsa-miR-30e*/mmu- FL miR-30e*/rno-miR- 30e* hcmv-miR-US25-1* FL
hsa-miR-708/mmu- FL miR-708/rno-miR-708 hsv1-miR-H1 FL
hsa-let-7g/mmu-let-7g FL hsa-miR-146a/mmu- FL miR-146a/rno-miR-
146a ebv-miR-BART8* FL hsa-miR-106b*/mmu- FL miR-106b*/rno-miR-
106b* hsa-miR-601 FL hsa-miR-553 FL hsa-miR-518b FL hsa-miR-548d-5p
FL hsa-miR-382/mmu- FL miR-382/rno-miR-382 hsa-miR-630 FL
hsa-miR-144* FL hsa-miR-519d FL mghv-miR-M1-3 FL hsa-miR-497/mmu-
FL miR-497/rno-miR-497 hsa-miR-524-5p FL hsa-miR-500* FL
hsa-miR-920 FL hsa-miR-297/mmu- FL miR-297a hsa-miR-509-3-5p FL
hsa-miR-340*/mmu- FL miR-340-3p/rno-miR- 340-3p hsa-miR-99b*/mmu-
FL miR-99b*/rno-miR- 99b* hsa-miR-887 FL hsa-miR-331-3p/mmu- FL
miR-331-3p/rno-miR- 331 hsa-miR-206/mmu- FL miR-206/rno-miR-206
hsa-miR-377* FL mghv-miR-M1-8 FL hsa-miR-513a-3p FL hsa-miR-146b-3p
FL hsa-miR-155* FL hsa-miR-574-3p/mmu- FL miR-574-3p
hsa-miR-615-3p/mmu- FL miR-615-3p hsa-miR-28-5p/mmu- FL
miR-28/rno-miR-28 hsa-miR-934 FL hsa-miR-151-5p/mmu- FL
miR-151-5p/rno-miR- 151 hsa-miR-885-5p FL hsa-miR-409-5p/mmu- FL
miR-409-5p/rno-miR- 409-5p hsa-let-7d*/mmu-let- FL 7d*/rno-let-7d*
hsa-miR-155 FL hsa-let-7b*/mmu-let- FL 7b*/rno-let-7b* hsa-miR-7-2*
FL hsa-miR-221* FL hsa-miR-9*/mmu-miR- FL 9*/rno-miR-9*
hsa-miR-122* FL hsa-miR-130b/mmu- FL miR-130b/rno-miR- 130b
hsa-miR-183/mmu- FL miR-183/rno-miR-183 hsa-miR-92a/mmu- FL
miR-92a/rno-miR-92a hsa-miR-890 FL hsa-miR-938 FL kshv-miR-K12-7 FL
hsa-miR-629* FL hsa-miR-922 FL kshv-miR-K12-5 FL hsa-miR-197/mmu-
FL miR-197 hsa-miR-552 FL hsa-miR-151-3p FL hsa-miR-194/mmu- FL
miR-194/rno-miR-194 hsa-miR-218-2*/mmu- FL miR-218-2*/rno-miR- 218*
hsa-miR-181b/mmu- FL miR-181b/rno-miR- 181b ebv-miR-BART18-3p FL
hsa-miR-34c-5p/mmu- FL miR-34c/rno-miR-34c
hsa-miR-622 FL hsa-miR-514 FL hsa-miR-657 FL hsa-miR-518a-3p FL
hsa-miR-647 FL hsa-miR-22*/mmu- FL miR-22*/rno-miR-22*
hsa-miR-196a*/mmu- FL miR-196a*/rno-miR- 196a* kshv-miR-K12-1 FL
hsa-miR-425/mmu- FL miR-425/rno-miR-425 hsa-miR-361-3p FL
hsa-miR-220b FL hsa-miR-744/mmu- FL miR-744 hsa-miR-551a FL
hsa-miR-301a/mmu- FL miR-301a/rno-miR- 301a hsa-miR-92b/mmu- FL
miR-92b/rno-miR-92b hsa-miR-487b/mmu- FL miR-487b/rno-miR- 487b
hsa-miR-363*/rno-miR- FL 363* hsa-miR-337-3p FL hsa-miR-636 FL
hsa-miR-600 FL hsa-miR-138/mmu- FL miR-138/rno-miR-138
hsa-miR-96/mmu-miR- FL 96/rno-miR-96 hsa-miR-20b* FL
hsa-miR-342-5p/mmu- FL miR-342-5p/rno-miR- 342-5p hsa-miR-215 FL
hsa-miR-129* FL
TABLE-US-00033 TABLE 27 Predictor microRNAs that distinguish
Burkitt lymphoma from Hodgkin's lymphoma Higher BL vs HL in
hsa-miR-19b/mmu-miR- BL 19b/rno-miR-19b hsa-miR-19a/mmu-miR- BL
19a/rno-miR-19a hsa-miR-17/mmu-miR- BL 17/rno-miR-17-5p/rno- miR-17
hsa-miR-106a BL hsa-miR-20a/mmu-miR- BL 20a/rno-miR-20a
hsa-miR-106b/mmu-miR- BL 106b/rno-miR-106b hsa-miR-30c/mmu-miR- BL
30c/rno-miR-30c hsa-miR-551b/mmu-miR- HL 551b/rno-miR-551b
hsa-miR-921 HL ebv-miR-BART2-3p HL hsa-miR-32* HL
hsa-miR-494/mmu-miR- HL 494/rno-miR-494 hsa-miR-29c/mmu-miR- HL
29c/rno-miR-29c hsa-miR-923 HL hsa-miR-199b-5p HL
hsa-miR-148a/mmu-miR- HL 148a hsa-miR-130a/mmu-miR- HL
130a/rno-miR-130a hsa-miR-154/mmu-miR- HL 154/rno-miR-154
hsa-miR-151-5p/mmu- HL miR-151-5p/rno-miR-151 hsa-miR-28-5p/mmu- HL
miR-28/rno-miR-28 hsa-miR-365/mmu-miR- HL 365/rno-miR-365
hsa-miR-602 HL hsa-miR-222/mmu-miR- HL 222/rno-miR-222
hsa-miR-214/mmu-miR- HL 214/rno-miR-214 hsa-miR-144* HL
hsa-miR-107/mmu-miR- HL 107/rno-miR-107 hsa-miR-497/mmu-miR- HL
497/rno-miR-497 hsa-let-7g/mmu-let-7g HL hsa-miR-146a/mmu-miR- HL
146a/rno-miR-146a hsa-miR-186/mmu-miR- HL 186/rno-miR-186
hsa-miR-886-5p HL hsa-miR-152/mmu-miR- HL 152/rno-miR-152
hsa-miR-29a*/mmu-miR- HL 29a*/rno-miR-29a* hsa-miR-140-5p/mmu- HL
miR-140/rno-miR-140 hsa-miR-532-5p/mmu- HL miR-532-5p/rno-miR-
532-5p hsa-miR-145*/mmu-miR- HL 145* hsa-miR-515-5p HL
hsa-miR-153/mmu-miR- HL 153/rno-miR-153 hsa-miR-513a-5p HL
hsa-miR-516a-5p HL hsa-miR-660 HL hsa-miR-29c*/mmu-miR- HL
29c*/rno-miR-29c* hsa-miR-505/rno-miR- HL 505 hsa-miR-455-3p HL
hsa-miR-519e* HL hsa-miR-502-3p HL hsa-miR-922 HL hsa-miR-524-5p HL
hsa-miR-483-5p HL hsa-miR-708/mmu-miR- HL 708/rno-miR-708
hsa-miR-498 HL ebv-miR-BART19-3p HL hsa-miR-149/mmu-miR- HL 149
hsa-miR-574-3p/mmu- HL miR-574-3p hsa-miR-659 HL
hsa-miR-331-3p/mmu- HL miR-331-3p/rno-miR-331 hsa-miR-105 HL
hsa-miR-128/mmu-miR- HL 128/rno-miR-128 hsa-miR-200b*/mmu- HL
miR-200b* hsa-miR-381/mmu-miR- HL 381/rno-miR-381 hsa-miR-766 HL
hsa-miR-557 HL ebv-miR-BART16 HL hsa-miR-488 HL hsa-miR-516b HL
mghv-miR-M1-2 HL hsa-miR-891a HL hsa-miR-221/mmu-miR- HL
221/rno-miR-221 hsa-miR-146b-3p HL hsa-miR-526b HL mghv-miR-M1-3 HL
hsa-miR-505* HL hsv1-miR-H1 HL hcmv-miR-UL70-3p HL
hsa-miR-24-2*/mmu- HL miR-24-2*/rno-miR-24- 2* hsa-miR-617 HL
hsa-miR-194/mmu-miR- HL 194/rno-miR-194 hsa-miR-934 HL hsa-miR-220c
HL hsa-miR-548d-5p HL hsa-miR-937 HL ebv-miR-BART13 HL
hsa-miR-7/mmu-miR- HL 7a/rno-miR-7a hsa-miR-210/mmu-miR- HL
210/rno-miR-210 hsa-miR-490-3p/mmu- HL miR-490 hsa-miR-221* HL
hsa-miR-92a/mmu-miR- HL 92a/rno-miR-92a hsa-miR-183*/mmu-miR- HL
183* hsa-miR-513a-3p HL hsa-miR-575 HL hsa-miR-595 HL hsa-miR-920
HL hsa-miR-483-3p HL hsa-miR-330-5p/mmu- HL miR-330/rno-miR-330
hsa-miR-525-5p HL hsa-miR-99b/mmu-miR- HL 99b/rno-miR-99b
hsa-miR-509-3p HL hsa-miR-151-3p HL ebv-miR-BHRF1-1 HL hsa-miR-630
HL mghv-miR-M1-7-3p HL hsa-miR-328/mmu-miR- HL 328/rno-miR-328
hsa-miR-452 HL hsa-miR-635 HL ebv-miR-BART5 HL hsa-miR-373* HL
hsa-miR-96/mmu-miR- HL 96/rno-miR-96 hsa-miR-382/mmu-miR- HL
382/rno-miR-382 hsa-miR-155 HL hsa-miR-197/mmu-miR- HL 197
kshv-miR-K12-6-5p HL hcmv-miR-UL112 HL hsa-miR-551b* HL
hsa-miR-877/mmu-miR- HL 877/rno-miR-877 hsa-miR-589 HL hsa-miR-936
HL hsa-miR-34c-5p/mmu- HL miR-34c/rno-miR-34c hsa-miR-885-5p HL
ebv-miR-BART6-3p HL hsa-miR-585 HL hsa-miR-302c* HL
hsa-miR-196a*/mmu- HL miR-196a*/rno-miR- 196a* hsa-miR-195* HL
hsa-miR-17*/rno-miR-17- HL 3p hsa-miR-296-5p/mmu- HL
miR-296-5p/rno-miR- 296* hsa-miR-550* HL ebv-miR-BHRF1-3 HL
hsa-miR-296-3p/mmu- HL miR-296-3p/rno-miR-296 hsa-miR-526b* HL
hsa-miR-548b-3p HL hsa-miR-652/mmu-miR- HL 652/rno-miR-652
hsa-miR-297/mmu-miR- HL 297a hsa-miR-553 HL hsa-miR-194* HL
hsa-miR-23a*/rno-miR- HL 23a* hsa-miR-130b/mmu-miR- HL
130b/rno-miR-130b hsa-miR-586 HL hsa-miR-137/mmu-miR- HL
137/rno-miR-137 hsa-miR-610 HL mghv-miR-M1-8 HL hsa-miR-193b* HL
hsa-miR-519d HL hsa-miR-125b-1*/mmu- HL miR-125b-3p/rno-miR-
125b-3p hsa-miR-744/mmu-miR- HL 744 hsa-miR-138/mmu-miR- HL
138/rno-miR-138 hsa-miR-21* HL hsa-miR-576-5p HL
hsa-miR-125a-3p/mmu- HL miR-125a-3p/rno-miR- 125a-3p mghv-miR-M1-6
HL hsa-miR-425/mmu-miR- HL 425/rno-miR-425 hsa-miR-10a*/mmu-miR- HL
10a*/rno-miR-10a-3p hsa-miR-215 HL hsa-miR-345 HL hsa-miR-887 HL
hsa-miR-7-2* HL hsa-miR-122* HL hsa-miR-363*/rno-miR- HL 363*
hsa-miR-22*/mmu-miR- HL 22*/rno-miR-22* hsa-miR-542-5p/mmu- HL
miR-542-5p/rno-miR- 542-5p hsa-miR-92b* HL hsa-miR-526a/hsa- HL
miR-520c-5p/hsa- miR-518d-5p kshv-miR-K12-5 HL hsa-miR- HL
340*/mmu-miR- 340-3p/rno-miR- 340-3p hsa-let-7d*/mmu- HL
let-7d*/rno-let-7d* hsa-miR-92b/mmu- HL miR-92b/rno-miR- 92b
hsa-miR-518b HL hiv1-miR-H1 HL hsa-miR-623 HL hsa-miR-645 HL
hsa-miR-601 HL hsa-miR-519e HL hsa-miR-650 HL hsa-miR-361-3p HL
hsa-miR- HL 150*/mmu-miR- 150* hsa-miR- HL 425*/mmu-miR- 425*
hsa-miR- HL 135a*/mmu-miR- 135a* hsa-miR-518a-3p HL hsa-miR-612 HL
hsa-miR-212/mmu- HL miR-212/rno-miR- 212 hsa-miR-125b- HL
2*/rno-miR-125b* hsa-miR-500 HL hsa-miR-663 HL hsa-miR-647 HL
hsa-miR-502-5p HL ebv-miR-BART18- HL 3p hsa-miR- HL 99b*/mmu-miR-
99b*/rno-miR-99b* ebv-miR-BART7* HL hsa-miR-500* HL hsa-miR- HL
124*/mmu-miR- 124*/rno-miR-124* hsa-miR-206/mmu- HL
miR-206/rno-miR- 206 hsa-miR-615- HL 3p/mmu-miR-615- 3p
hsa-miR-30c- HL 1*/mmu-miR-30c- 1*/rno-miR-30c-1* hsa-miR-331- HL
5p/mmu-miR-331- 5p hcmv-miR-US25-1* HL hsa-miR-326/mmu- HL
miR-326/rno-miR- 326 hsa-miR- HL 181b/mmu-miR- 181b/rno-miR-181b
hsa-miR-376a* HL hsa-miR-433/mmu- HL miR-433/rno-miR- 433
hsa-miR-640 HL hsa-miR-938 HL hsa-miR-508-5p HL hsa-miR-484/mmu- HL
miR-484/rno-miR- 484 hsa-miR- HL 106b*/mmu-miR- 106b*/rno-miR-
106b* hsa-miR-600 HL hsa-let-7b*/mmu- HL let-7b*/rno-let-7b*
hsa-miR-20b* HL hsa-miR-622 HL hsa-miR-657 HL hsa-miR-183/mmu- HL
miR-183/rno-miR- 183 hsa-miR-409- HL 5p/mmu-miR-409-
5p/rno-miR-409-5p ebv-miR-BART20- HL 3p ebv-miR-BART8* HL
hsa-miR-129* HL hsa-miR-629 HL hsa-miR-890 HL hsa-miR-208a/mmu- HL
miR-208a/rno-miR- 208 hsa-miR-492 HL hsa-miR-629* HL hsa-miR-654-5p
HL hsa-miR-584 HL hsa-miR-636 HL hsa-miR-490-5p HL hsa-miR-337-3p
HL hsa-miR-9*/mmu- HL miR-9*/rno-miR-9* ebv-miR-BART9* HL
hsa-miR-509-3-5p HL hsa-miR-342- HL 5p/mmu-miR-342-
5p/rno-miR-342-5p hsa-miR-514 HL hsa-miR-377* HL kshv-miR-K12-1 HL
hsa-miR-124/mmu- HL miR-124/rno-miR- 124 hsa-miR-542- HL
3p/mmu-miR-542- 3p/rno-miR-542-3p hsa-miR-220b HL hsa-miR-299-3p HL
hsa-miR-181a-2* HL hsa-miR-202 HL hsa-miR- HL 487b/mmu-miR-
487b/rno-miR-487b hsa-miR-551a HL hsa-miR-338- HL 5p/mmu-miR-338-
5p/rno-miR-338* hsa-miR-552 HL
TABLE-US-00034 TABLE 28 Predictor microRNAs that distinguish
chronic lymphocytic leukemia from follicular lymphoma Higher CLL vs
FL in hsa-miR-331-5p/mmu- CLL miR-331-5p hsa-miR-144/mmu-miR- CLL
144/rno-miR-144 hsa-miR-150/mmu-miR- CLL 150/rno-miR-150
hsa-miR-140-5p/mmu- CLL miR-140/rno-miR-140 hsa-miR-335/mmu-miR-
CLL 335-5p/rno-miR-335 hsa-miR-186/mmu-miR- CLL 186/rno-miR-186
hsa-miR-486-5p/mmu- CLL miR-486 hsa-miR-154/mmu-miR- CLL
154/rno-miR-154 hsa-miR-223/mmu-miR- CLL 223/rno-miR-223
hsa-miR-299-5p/mmu- CLL miR-299*/rno-miR-299 hsa-let-7g/mmu-let-7g
CLL hsa-miR-32/mmu-miR- CLL 32/rno-miR-32 hsa-miR-30e*/mmu-miR- CLL
30e*/rno-miR-30e* hsa-miR-147 CLL hsa-miR-20b/mmu-miR- CLL
20b/rno-miR-20b-5p hsa-miR-101/mmu-miR- CLL 101a/rno-miR-101a
hsa-let-7f/mmu-let-7f/rno- CLL let-7f hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e hsa-miR-668/mmu-miR- CLL 668 hsa-miR-768-5p CLL
hsa-miR-19a/mmu-miR- CLL 19a/rno-miR-19a hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR- 199a-5p hsa-miR-638 CLL hsa-miR-196a*/mmu-miR-
CLL 196a*/rno-miR-196a* hsa-miR-19b/mmu-miR- CLL 19b/rno-miR-19b
hsa-miR-30d/mmu-miR- CLL 30d/rno-miR-30d hsa-miR-363/mmu-miR- CLL
363/rno-miR-363 hsa-miR-374a CLL hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR-140* hsa-miR-185/mmu-miR- CLL 185/rno-miR-185
hsa-miR-106b/mmu-miR- CLL 106b/rno-miR-106b hsa-miR-106a CLL
hsa-miR-191/mmu-miR- CLL 191/rno-miR-191 hsa-miR-17/mmu-miR- CLL
17/rno-miR-17-5p/rno- miR-17 hsa-let-7i/mmu-let-7i/rno- CLL let-7i
hsa-miR-20a/mmu-miR- CLL 20a/rno-miR-20a hsa-miR-142-5p/mmu- CLL
miR-142-5p/rno-miR-142- 5p hsa-miR-768-3p CLL hsa-miR-30b/mmu-miR-
CLL 30b/rno-miR-30b-5p hsa-miR-891a CLL hsa-miR-24-1*/mmu-miR- CLL
24-1*/rno-miR-24-1* hsa-miR-29c/mmu-miR- CLL 29c/rno-miR-29c
hsa-miR-28-5p/mmu-miR- CLL 28/rno-miR-28 hsa-miR-30a/mmu-miR- CLL
30a/rno-miR-30a hsa-miR-155 CLL hsa-miR-361-5p/mmu- CLL
miR-361/rno-miR-361 hsa-miR-15a/mmu-miR- CLL 15a
hsa-miR-26a/mmu-miR- CLL 26a/rno-miR-26a hsa-miR-30c/mmu-miR- CLL
30c/rno-miR-30c hsa-miR-541* CLL hsa-miR-26b/mmu-miR- CLL
26b/rno-miR-26b hsa-miR-519d CLL hsa-miR-15b/mmu-miR- CLL
15b/rno-miR-15b hsa-miR-550 CLL hsa-miR-29b/mmu-miR- CLL
29b/rno-miR-29b hsa-miR-29a/mmu-miR- CLL 29a/rno-miR-29a
hsa-miR-103/mmu-miR- CLL 103/rno-miR-103 hsa-miR-423-3p/mmu- CLL
miR-423-3p/rno-miR-423 hsa-miR-549 CLL hsa-miR-107/mmu-miR- CLL
107/rno-miR-107 hsa-miR-888* CLL hsa-miR-801/mmu-miR- CLL 801
hsa-miR-149* FL hsa-miR-634 FL ebv-miR-BART2-3p FL hsa-miR-921 FL
hsa-miR-494/mmu-miR- FL 494/rno-miR-494 hsa-miR-933 FL
mghv-miR-M1-4 FL hsa-let-7e/mmu-let-7e/rno- FL let-7e hsa-miR-939
FL hsa-miR-518c* FL hsa-miR-32* FL hsa-miR-491-3p FL
hsa-miR-185/mmu-miR- FL 185/rno-miR-185 hsa-miR-765 FL
ebv-miR-BART17-5p FL hsa-miR-576-3p FL hsa-miR-658 FL hsa-miR-503
FL hsa-miR-30b* FL hsa-miR-302a/mmu-miR- FL 302a hsa-miR-628-3p FL
hsa-miR-642 FL hsa-miR-620 FL hsa-miR-99a/mmu- FL miR-99a/rno-miR-
99a hsa-miR-371-5p FL hsa-miR-452 FL hsa-miR-126*/mmu- FL
miR-126-5p/rno- miR-126* hsa-miR-298 FL hsa-miR-193a-5p FL
hsa-miR-583 FL hsa-miR-143/mmu- FL miR-143/rno-miR- 143 hsa-miR-665
FL hsa-miR-505/rno- FL miR-505 hsa-miR-199b-5p FL
hsa-miR-28-3p/rno- FL miR-28* hsa-miR-422a FL hsa-miR-515-5p FL
hsa-miR-455-3p FL hsa-miR-10a/mmu- FL miR-10a/rno-miR- 10a-5p
hsa-miR-300 FL ebv-miR-BART5 FL hsa-miR-10b/mmu- FL
miR-10b/rno-miR- 10b hsa-miR-212/mmu- FL miR-212/rno-miR- 212
hsa-miR-145/mmu- FL miR-145/rno-miR- 145 hsa-miR-187* FL
ebv-miR-BHRF1-1 FL ebv-miR-BHRF1-2 FL hsa-miR-126/mmu- FL
miR-126-3p/rno- miR-126 hsa-miR- FL 130b*/mmu-miR- 130b*
hsa-miR-326/mmu- FL miR-326/rno-miR- 326 mghv-miR-M1-2 FL
kshv-miR-K12-6-3p FL hsa-miR-516b FL hsa-miR-519e* FL
mghv-miR-M1-7-3p FL hsa-miR-629 FL hsa-miR-24-2*/mmu- FL
miR-24-2*/rno-miR- 24-2* hsa-miR-943 FL hsa-miR-124/mmu- FL
miR-124/rno-miR- 124 hsa-miR-365/mmu- FL miR-365/rno-miR- 365
hsa-miR-493 FL hsa-miR-29c*/mmu- FL miR-29c*/rno-miR- 29c*
hsa-miR-602 FL hsa-miR-484/mmu- FL miR-484/rno-miR- 484
hsa-miR-483-3p FL hsa-miR-125b- FL 2*/rno-miR-125b* hsa-miR-675 FL
mghv-miR-M1-7-5p FL hsa-miR-152/mmu- FL miR-152/rno-miR- 152
hsa-miR-27a*/mmu- FL miR-27a*/rno-miR- 27a* hsa-miR-542- FL
3p/mmu-miR-542- 3p/rno-miR-542-3p hsa-miR-100/mmu- FL
miR-100/rno-miR- 100 hsa-miR-208a/mmu- FL miR-208a/rno-miR- 208
hsa-miR-766 FL hsa-miR-637 FL hsa-miR-519c- FL 5p/hsa-miR-519b-
5p/hsa-miR- 523*/hsa-miR- 518e*/hsa-miR- 522*/hsa-miR-519a*
hsa-miR-409- FL 5p/mmu-miR-409-
5p/rno-miR-409-5p hsa-miR-199a- FL 3p/hsa-miR-199b-
3p/mmu-miR-199a- 3p/mmu-miR- 199b/rno-miR-199a- 3p hsa-miR- FL
106b*/mmu-miR- 106b*/rno-miR- 106b* hsa-miR-130a/mmu- FL
miR-130a/rno-miR- 130a hsa-miR-645 FL hsa-miR-548d-5p FL
hsa-miR-671- FL 5p/mmu-miR-671-5p hsa-miR-574- FL 3p/mmu-miR-574-3p
hsa-miR-125b/mmu- FL miR-125b-5p/rno- miR-125b-5p hsa-miR-500* FL
hsa-miR-425/mmu- FL miR-425/rno-miR- 425 ebv-miR-BART8* FL
hsa-miR-377* FL hsa-miR-513a-3p FL hsa-miR-181a-2* FL hsa-miR-938
FL hsa-miR-155* FL hsa-miR-218- FL 2*/mmu-miR-218- 2*/rno-miR-218*
hsa-miR-198 FL hsa-miR-151-3p FL hsa-miR-299-3p FL hcmv-miR-US25-1*
FL hsa-miR-708/mmu- FL miR-708/rno-miR- 708 hsa-miR-659 FL
hsa-miR-600 FL hsa-miR-601 FL hsa-miR-296- FL 3p/mmu-miR-296-
3p/rno-miR-296 hsa-let-7b*/mmu-let- FL 7b*/rno-let-7b*
ebv-miR-BART6-3p FL hsa-miR-7-2* FL hsa-miR-9*/mmu- FL
miR-9*/rno-miR-9* hsa-miR-509-3-5p FL hsa-miR-518b FL
hsa-miR-183*/mmu- FL miR-183* hsa-miR-125b- FL 1*/mmu-miR-125b-
3p/rno-miR-125b-3p hsa-miR-183/mmu- FL miR-183/rno-miR- 183
hsa-miR-890 FL hsa-miR-153/mmu- FL miR-153/rno-miR- 153
hsa-miR-874/mmu- FL miR-874/rno-miR- 874 hsa-miR-220c FL
hsa-miR-99b*/mmu- FL miR-99b*/rno-miR- 99b* hsa-miR-193b* FL
hsa-miR-629* FL hcmv-miR-UL148D FL ebv-miR-BART7* FL
hsa-miR-99b/mmu- FL miR-99b/rno-miR- 99b hsa-miR-206/mmu- FL
miR-206/rno-miR- 206 hsa-miR-381/mmu- FL miR-381/rno-miR- 381
hsa-miR-194/mmu- FL miR-194/rno-miR- 194 hsa-miR-525-5p FL
hsa-miR-193b FL hsa-miR-497/mmu- FL miR-497/rno-miR- 497
ebv-miR-BART18- FL 3p hsa-miR-424 FL hsa-miR-553 FL
hsa-let-7d*/mmu-let- FL 7d*/rno-let-7d* hsa-miR-34c- FL 5p/mmu-miR-
34c/rno-miR-34c ebv-miR-BHRF1-3 FL kshv-miR-K12-6-5p FL
hsa-miR-551a FL hsa-miR-195* FL hsa-miR-551b* FL hsa-miR-514 FL
hsa-miR-552 FL hsa-miR-122* FL hsa-miR-92b/mmu- FL miR-92b/rno-miR-
92b hsa-miR-22*/mmu- FL miR-22*/rno-miR- 22* hsa-miR-635 FL
kshv-miR-K12-1 FL hsa-miR-483-5p FL hsa-miR-340*/mmu- FL
miR-340-3p/rno- miR-340-3p hsa-miR-615- FL 3p/mmu-miR-615-3p
hsa-miR-505* FL hsa-miR-622 FL hsa-miR-181b/mmu- FL
miR-181b/rno-miR- 181b hsa-miR-886-5p FL hsa-miR-885-5p FL
hsa-miR-220b FL hsa-miR-524-5p FL hsa-miR-382/mmu- FL
miR-382/rno-miR- 382 hsa-miR-744/mmu- FL miR-744 hsv1-miR-H1 FL
hsa-miR-526b FL hsa-miR-657 FL hsa-miR-130b/mmu- FL
miR-130b/rno-miR- 130b hsa-miR-181a/mmu- FL miR-181a/rno-miR- 181a
hsa-miR-301a/mmu- FL miR-301a/rno-miR- 301a hsa-miR-490- FL
3p/mmu-miR-490 hsa-miR-485- FL 3p/mmu-miR-485* hsa-miR-297/mmu- FL
miR-297a hsa-miR-630 FL hsa-miR-877/mmu- FL miR-877/rno-miR- 877
kshv-miR-K12-5 FL hsa-miR-617 FL mghv-miR-M1-3 FL hsa-miR-920 FL
hsa-miR-585 FL hsa-miR-374b* FL hsa-miR-215 FL hsa-miR-342- FL
5p/mmu-miR-342- 5p/rno-miR-342-5p hsa-miR-934 FL hsa-miR-575 FL
hsa-miR-488 FL ebv-miR-BART16 FL hsa-miR-647 FL hsa-miR-138/mmu- FL
miR-138/rno-miR- 138 hsa-miR-221* FL hsa-miR- FL 200b*/mmu-miR-
200b* hsa-miR-337-3p FL hsa-miR-922 FL hsa-miR-197/mmu- FL miR-197
hsa-miR-96/mmu- FL miR-96/rno-miR-96 hsa-miR-518a-3p FL
TABLE-US-00035 TABLE 29 Predictor microRNAs that distinguish
Burkitt lymphoma from Hodgkin's lymphoma Higher CLL vs HL in
hsa-miR-32/mmu-miR- CLL 32/rno-miR-32 hsa-miR-30e*/mmu-miR- CLL
30e*/rno-miR-30e* hsa-let-7g/mmu-let-7g CLL hsa-miR-144/mmu-miR-
CLL 144/rno-miR-144 hsa-miR-140-5p/mmu- CLL miR-140/rno-miR-140
hsa-miR-19a/mmu-miR- CLL 19a/rno-miR-19a hsa-miR-154/mmu-miR- CLL
154/rno-miR-154 hsa-miR-150/mmu-miR- CLL 150/rno-miR-150
hsa-miR-28-5p/mmu- CLL miR-28/rno-miR-28 hsa-miR-363/mmu-miR- CLL
363/rno-miR-363 hsa-miR-101/mmu-miR- CLL 101a/rno-miR-101a
hsa-miR-299-5p/mmu- CLL miR-299*/rno-miR-299 hsa-miR-768-5p CLL
hsa-miR-19b/mmu-miR- CLL 19b/rno-miR-19b hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e hsa-miR-20b/mmu-miR- CLL 20b/rno-miR-20b-5p
hsa-miR-374a CLL hsa-let-7f/mmu-let-7f/rno- CLL let-7f
hsa-miR-335/mmu-miR- CLL 335-5p/rno-miR-335 hsa-miR-142-5p/mmu- CLL
miR-142-5p/rno-miR-142- 5p hsa-miR-486-5p/mmu- CLL miR-486
hsa-miR-33a/mmu-miR- CLL 33/rno-miR-33 hsa-miR-30b/mmu-miR- CLL
30b/rno-miR-30b-5p hsa-miR-768-3p CLL hsa-miR-668/mmu-miR- CLL 668
hsa-miR-15b/mmu-miR- CLL 15b/rno-miR-15b hsa-miR-196a*/mmu- CLL
miR-196a*/rno-miR-196a* hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR-140* hsa-miR-29b/mmu-miR- CLL 29b/rno-miR-29b
hsa-miR-29c/mmu-miR- CLL 29c/rno-miR-29c hsa-miR-186/mmu-miR- CLL
186/rno-miR-186 hsa-miR-106a CLL hsa-miR-26a/mmu-miR- CLL
26a/rno-miR-26a hsa-miR-106b/mmu-miR- CLL 106b/rno-miR-106b
hsa-miR-17/mmu-miR- CLL 17/rno-miR-17-5p/rno- miR-17
hsa-miR-191/mmu-miR- CLL 191/rno-miR-191 hsa-miR-20a/mmu-miR- CLL
20a/rno-miR-20a hsa-miR-30c/mmu-miR- CLL 30c/rno-miR-30c
hsa-miR-26b/mmu-miR- CLL 26b/rno-miR-26b hsa-miR-147 CLL
hsa-miR-15a/mmu-miR- CLL 15a hsa-miR-30d/mmu-miR- CLL
30d/rno-miR-30d hsa-miR-199a-5p/mmu- CLL miR-199a-5p/rno-miR-
199a-5p hsa-miR-29a/mmu-miR- CLL 29a/rno-miR-29a
hsa-miR-223/mmu-miR- CLL 223/rno-miR-223 hsa-miR-30a/mmu-miR- CLL
30a/rno-miR-30a hsa-miR-16/mmu-miR- CLL 16/rno-miR-16
hsa-miR-451/mmu-miR- CLL 451/rno-miR-451 hsa-miR-24-1*/mmu-miR- CLL
24-1*/rno-miR-24-1* hsa-miR-550 CLL hsa-miR-342-3p/mmu- CLL
miR-342-3p/rno-miR-342- 3p hsa-miR-195/mmu-miR- CLL 195/rno-miR-195
hsa-miR-801/mmu-miR- CLL 801 hsa-miR-541* CLL
hsa-let-7i/mmu-let-7i/rno- CLL let-7i hsa-miR-155 CLL
hsa-miR-185/mmu-miR- CLL 185/rno-miR-185 hsa-miR-891a CLL
hsa-miR-138-1*/mmu- CLL miR-138*/rno-miR-138* hsa-miR-27b/mmu-miR-
CLL 27b/rno-miR-27b hsa-miR-361-5p/mmu- CLL miR-361/rno-miR-361
hsa-miR-129* CLL hsa-miR-638 CLL hsa-miR-34b/mmu-miR- CLL 34b-3p
hsa-miR-107/mmu-miR- CLL 107/rno-miR-107 hsa-miR-549 CLL
hsa-miR-888* CLL hsa-miR-423-3p/mmu- CLL miR-423-3p/rno-miR-423
hsa-let-7e/mmu-let- HL 7e/rno-let-7e hsa-miR-125a-5p/mmu- HL
miR-125a-5p/rno-miR- 125a-5p hsa-miR-576-3p HL hsa-miR-513a-5p HL
ebv-miR-BART17-5p HL hsa-miR-185/mmu-miR- HL 185/rno-miR-185
hsa-miR-921 HL hsa-miR-518c* HL hsa-miR-520d-5p HL hsa-miR-939 HL
hsa-miR-634 HL hsa-miR-491-3p HL ebv-miR-BART2-3p HL
hsa-miR-30c-2*/mmu- HL miR-30c-2*/rno-miR-30c- 2* hsa-miR-765 HL
hsa-miR-923 HL hsa-miR-620 HL hsa-miR-933 HL hsa-miR-143/mmu-miR-
HL 143/rno-miR-143 hsa-miR-494/mmu-miR- HL 494/rno-miR-494
hsa-miR-665 HL hsa-miR-642 HL hsa-miR-126*/mmu-miR- HL
126-5p/rno-miR-126* hsa-miR-658 HL hsa-miR-149* HL hsa-miR-30b* HL
mghv-miR-M1-4 HL hsa-miR-99a/mmu-miR- HL 99a/rno-miR-99a
hsa-miR-193a-5p HL hsa-miR-498 HL hsa-miR-628-3p HL hsa-miR-185* HL
hsa-miR-371-5p HL hsa-miR-199b-5p HL hsa-miR-126/mmu-miR- HL
126-3p/rno-miR-126 hsa-miR-503 HL hsa-miR-10a/mmu-miR- HL
10a/rno-miR-10a-5p hsa-miR-300 HL hsa-miR-583 HL
hsa-miR-518a-5p/hsa- HL miR-527 hsa-miR-10b/mmu-miR- HL
10b/rno-miR-10b hsa-miR-145/mmu- HL miR-145/rno-miR- 145
hsa-miR-128/mmu- HL miR-128/rno-miR- 128 hsa-miR-532- HL
5p/mmu-miR-532- 5p/rno-miR-532-5p hsa-miR-143* HL
hsa-miR-28-3p/rno- HL miR-28* hsa-miR- HL 130b*/mmu-miR- 130b*
hsa-miR-505/rno- HL miR-505 hsa-miR-25* HL hsa-miR-574- HL
3p/mmu-miR-574-3p hsa-miR-455-3p HL kshv-miR-K12-3 HL hsa-miR-516b
HL kshv-miR-K12-8 HL hsa-miR-502-3p HL kshv-miR-K12-6-3p HL
hsa-miR-129- HL 5p/mmu-miR-129- 5p/rno-miR-129 hsa-miR-515-5p HL
hsa-miR-199a- HL 3p/hsa-miR-199b- 3p/mmu-miR-199a- 3p/mmu-miR-
199b/rno-miR-199a- 3p hsa-miR-149/mmu- HL miR-149 hsa-miR-889 HL
hsa-miR-637 HL hsa-miR-600 HL hsa-miR-151-3p HL hsa-miR-656 HL
hsa-miR-497/mmu- HL miR-497/rno-miR- 497 hsa-miR-152/mmu- HL
miR-152/rno-miR- 152 hsa-miR-100/mmu- HL miR-100/rno-miR- 100
hsa-miR-425/mmu- HL miR-425/rno-miR- 425 hsa-miR-145*/mmu- HL
miR-145* hsa-miR-365/mmu- HL miR-365/rno-miR- 365 hsa-miR-422a HL
hcmv-miR-UL70-3p HL
hsa-miR-27a*/mmu- HL miR-27a*/rno-miR- 27a* hsa-miR-194/mmu- HL
miR-194/rno-miR- 194 hsa-miR-548d-5p HL hsa-miR-187* HL
hsa-miR-323- HL 3p/mmu-miR-323- 3p/rno-miR-323 hsa-miR-708/mmu- HL
miR-708/rno-miR- 708 hsa-miR-29c*/mmu- HL miR-29c*/rno-miR- 29c*
hsa-miR-513a-3p HL hsa-miR-595 HL hsa-miR-483-3p HL hsa-miR-330- HL
5p/mmu-miR- 330/rno-miR-330 hsa-miR-519e* HL hsa-miR-509-3p HL
hsa-miR-328/mmu- HL miR-328/rno-miR- 328 hsa-miR-373* HL
hsa-miR-96/mmu- HL miR-96/rno-miR-96 hsa-miR-215 HL hsa-miR-589 HL
hsa-miR-34c- HL 5p/mmu-miR- 34c/rno-miR-34c hsa-miR-125b/mmu- HL
miR-125b-5p/rno- miR-125b-5p hsa-miR-130a/mmu- HL miR-130a/rno-miR-
130a hsa-miR-519c- HL 5p/hsa-miR-519b- 5p/hsa-miR- 523*/hsa-miR-
518e*/hsa-miR- 522*/hsa-miR-519a* mghv-miR-M1-7-5p HL
hsa-miR-516a-5p HL hsa-miR-424 HL hsa-miR-17*/rno- HL miR-17-3p
hsa-miR-296- HL 5p/mmu-miR-296- 5p/rno-miR-296* hsa-miR-550* HL
hsa-miR-210/mmu- HL miR-210/rno-miR- 210 hsa-miR-92b/mmu- HL
miR-92b/rno-miR- 92b hsa-miR-548b-3p HL hsa-miR-652/mmu- HL
miR-652/rno-miR- 652 hsa-miR-138/mmu- HL miR-138/rno-miR- 138
hsa-miR-194* HL hsa-miR-23a*/rno- HL miR-23a* hsa-miR-153/mmu- HL
miR-153/rno-miR- 153 hsa-miR-586 HL hsa-miR-137/mmu- HL
miR-137/rno-miR- 137 hsa-miR-610 HL hsa-miR-381/mmu- HL
miR-381/rno-miR- 381 hsa-miR-936 HL hsa-miR-744/mmu- HL miR-744
ebv-miR-BART5 HL ebv-miR-BHRF1-1 HL hsa-miR-21* HL hsa-miR-576-5p
HL mghv-miR-M1-6 HL hsa-miR-193b HL hsa-miR-10a*/mmu- HL
miR-10a*/rno-miR- 10a-3p hsa-miR-524-5p HL hsa-miR-452 HL
hsa-miR-345 HL hsa-miR-7-2* HL hsa-miR-409- HL 5p/mmu-miR-409-
5p/rno-miR-409-5p hsa-miR-557 HL hsa-miR-181a/mmu- HL
miR-181a/rno-miR- 181a hsa-miR-22*/mmu- HL miR-22*/rno-miR-22*
hsa-miR-922 HL hsa-miR-92b* HL hsa-miR-938 HL hsa-miR-526a/hsa- HL
miR-520c-5p/hsa- miR-518d-5p hsa-miR-526b* HL ebv-miR-BHRF1-2 HL
hiv1-miR-H1 HL hsa-miR-623 HL mghv-miR-M1-2 HL mghv-miR-M1-7-3p HL
hsa-miR-519e HL hsa-miR-650 HL hsa-miR-766 HL hsa-miR-602 HL
hsa-miR-425*/mmu- HL miR-425* hsa-miR- HL 135a*/mmu-miR- 135a* HL
hsa-miR-612 HL hsa-miR-212/mmu- HL miR-212/rno-miR- 212
hsa-miR-125b- HL 2*/rno-miR-125b* hcmv-miR-UL112 HL hsa-miR-374b*
hsa-miR-886-5p HL hsa-miR-500 HL hsa-miR-502-5p HL ebv-miR-BART18-
HL 3p hsa-miR-198 HL hsa-miR-500* HL hsa-miR-342- HL
5p/mmu-miR-342- 5p/rno-miR-342-5p hsa-miR-124*/mmu- HL
miR-124*/rno-miR- 124* hsa-miR-30c- HL 1*/mmu-miR-30c-
1*/rno-miR-30c-1* hsa-miR-220c HL hsa-miR-376a* HL hsa-miR-640 HL
hcmv-miR-UL148D HL hsa-miR-659 HL hsa-miR-934 HL hsa-miR-125a- HL
3p/mmu-miR-125a- 3p/rno-miR-125a-3p hsa-miR-885-5p HL hsa-miR-24-
HL 2*/mmu-miR-24- 2*/rno-miR-24-2* hsa-miR-484/mmu- HL
miR-484/rno-miR- 484 hsa-miR- HL 106b*/mmu-miR- 106b*/rno-miR-
106b* hsa-miR-505* HL hsa-let-7b*/mmu-let- HL 7b*/rno-let-7b*
hsa-miR-302c* HL hsa-miR-542- HL 5p/mmu-miR-542- 5p/rno-miR-542-5p
hsv1-miR-H1 HL mghv-miR-M1-3 HL hsa-miR-183/mmu- HL
miR-183/rno-miR- 183 hsa-miR-122* HL hsa-miR-183*/mmu- HL miR-183*
hsa-miR-675 HL hsa-miR-99b/mmu- HL miR-99b/rno-miR- 99b
hsa-miR-874/mmu- HL miR-874/rno-miR- 874 ebv-miR-BART20- HL 3p
hsa-miR-483-5p HL hsa-miR-671- HL 5p/mmu-miR-671-5p hsa-miR-629 HL
hsa-miR-553 HL hsa-let-7d*/mmu-let- HL 7d*/rno-let-7d* hsa-miR-601
HL hsa-miR-645 HL hsa-miR-920 HL hsa-miR-525-5p HL hsa-miR-221* HL
hsa-miR-890 HL hsa-miR-492 HL hsa-miR-629* HL hsa-miR-635 HL
hsa-miR-130b/mmu- HL miR-130b/rno-miR- 130b hsa-miR-197/mmu- HL
miR-197 hsa-miR-654-5p HL hsa-miR-518b HL hsa-miR-382/mmu- HL
miR-382/rno-miR- 382 hsa-miR-584 HL hsa-miR-99b*/mmu- HL
miR-99b*/rno-miR- 99b* hsa-miR-630 HL hsa-miR-490-5p HL hsa-miR-663
HL hsa-miR-337-3p HL hsa-miR-9*/mmu- HL miR-9*/rno-miR-9*
hsa-miR-202 HL ebv-miR-BART16 HL ebv-miR-BART9* HL hsa-miR-193b* HL
ebv-miR-BART8* HL hsa-miR-206/mmu- HL miR-206/rno-miR- 206
hcmv-miR-US25-1* HL hsa-miR-514 HL kshv-miR-K12-6-5p HL hsa-miR-488
HL hsa-miR-508-5p HL hsa-miR-551b* HL hsa-miR-377* HL
ebv-miR-BART6-3p HL hsa-miR-181b/mmu- HL
miR-181b/rno-miR- 181b hsa-miR-526b HL hsa-miR-622 HL
kshv-miR-K12-1 HL hsa-miR-485- HL 3p/mmu-miR-485* hsa-miR-490- HL
3p/mmu-miR-490 hsa-miR-125b- HL 1*/mmu-miR-125b- 3p/rno-miR-125b-3p
hsa-miR-124/mmu- HL miR-124/rno-miR- 124 hsa-miR-657 HL
ebv-miR-BHRF1-3 HL hsa-miR-542- HL 3p/mmu-miR-542-
3p/rno-miR-542-3p kshv-miR-K12-5 HL hsa-miR-943 HL hsa-miR-551a HL
hsa-miR-297/mmu- HL miR-297a hsa-miR-296- HL 3p/mmu-miR-296-
3p/rno-miR-296 hsa-miR-617 HL hsa-miR-195* HL hsa-miR-575 HL
hsa-miR-208a/mmu- HL miR-208a/rno-miR- 208 hsa-miR-647 HL
hsa-miR-509-3-5p HL hsa-miR-340*/mmu- HL miR-340-3p/rno- miR-340-3p
hsa-miR-220b HL hsa-miR- HL 200b*/mmu-miR- 200b* hsa-miR-585 HL
hsa-miR-877/mmu- HL miR-877/rno-miR- 877 hsa-miR-326/mmu- HL
miR-326/rno-miR- 326 ebv-miR-BART7* HL hsa-miR-615- HL
3p/mmu-miR-615-3p hsa-miR-433/mmu- HL miR-433/rno-miR- 433
hsa-miR-338- HL 5p/mmu-miR-338- 5p/rno-miR-338* hsa-miR-299-3p HL
hsa-miR-518a-3p HL hsa-miR-181a-2* HL hsa-miR-552 HL
TABLE-US-00036 TABLE 30 Predictor microRNAs that distinguish
follicular lymphoma from Hodgkin's lymphoma Higher FL vs HL in
hsa-miR-301a/mmu-miR- FL 301a/rno-miR-301a kshv-miR-K12-7 FL
hsa-miR-96/mmu-miR- FL 96/rno-miR-96 hsa-miR-151-5p/mmu- FL
miR-151-5p/rno-miR-151 hsa-miR-28-5p/mmu-miR- FL 28/rno-miR-28
hsa-miR-302a/mmu-miR- FL 302a hsa-miR-215 FL hsa-miR-15b/mmu-miR-
FL 15b/rno-miR-15b hsa-miR-29b/mmu-miR- FL 29b/rno-miR-29b
hsa-miR-138/mmu-miR- FL 138/rno-miR-138 hsa-miR-363/mmu-miR- FL
363/rno-miR-363 hsa-miR-142-5p/mmu- FL miR-142-5p/rno-miR-142- 5p
hsa-miR-19a/mmu-miR- FL 19a/rno-miR-19a hsa-miR-497/mmu-miR- FL
497/rno-miR-497 hsa-miR-144* FL hsa-miR-16/mmu-miR- FL
16/rno-miR-16 hsa-miR-138-1*/mmu- FL miR-138*/rno-miR-138*
hsa-miR-768-5p FL hsa-miR-30c/mmu-miR- FL 30c/rno-miR-30c
hsa-miR-129* FL hsa-miR-801/mmu-miR- FL 801 hsa-miR-34b/mmu-miR- FL
34b-3p hsa-miR-363*/rno-miR- FL 363* hsa-miR-20b* FL hsa-miR-550 FL
hsa-miR-600 FL hsa-miR-196a*/mmu- FL miR-196a*/rno-miR-196a*
hsa-miR-574-3p/mmu- FL miR-574-3p hsa-miR-620 FL
hsa-miR-331-3p/mmu- FL miR-331-3p/rno-miR-331 hsa-let-7e/mmu-let-
FL 7e/rno-let-7e hsa-miR-524-5p FL hsa-miR-197/mmu-miR- FL 197
hsa-miR-24-1*/mmu-miR- FL 24-1*/rno-miR-24-1* hsa-miR-519e* HL
hsa-miR-628-3p HL mghv-miR-M1-7-5p HL hsa-miR-498 HL hsa-miR-525-5p
HL hsa-miR-520d-5p HL hsa-miR-551b* HL hsa-miR-340*/mmu- HL
miR-340-3p/rno- miR-340-3p hsa-miR-889 HL hsa-miR-494/mmu- HL
miR-494/rno-miR- 494 hsa-miR-874/mmu- HL miR-874/rno-miR- 874
hsa-miR-30c- HL 2*/mmu-miR-30c- 2*/rno-miR-30c-2* hsa-miR-183*/mmu-
HL miR-183* hsa-miR-25* HL hsa-miR-513a-5p HL hsa-miR-198 HL
hsa-miR-659 HL mghv-miR-M1-4 HL hsa-miR-129- HL 5p/mmu-miR-129-
5p/rno-miR-129 ebv-miR-BART13 HL hsa-miR-193b* HL hsa-miR-422a HL
hsa-miR-503 HL kshv-miR-K12-3 HL hsa-miR-766 HL hsa-miR-516a-5p HL
hsa-miR-125b- HL 1*/mmu-miR-125b- 3p/rno-miR-125b-3p hsa-miR-149*
HL ebv-miR-BART6-3p HL ebv-miR-BART19-3p HL hsa-miR-671- HL
5p/mmu-miR-671-5p ebv-miR-BART8* HL hsa-miR-509-3-5p HL hsa-miR-602
HL ebv-miR-BHRF1-1 HL mghv-miR-M1-7-3p HL mghv-miR-M1-2 HL
hsa-miR-675 HL ebv-miR-BHRF1-2 HL hsa-miR-145*/mmu- HL miR-145*
hsa-miR-296-5p/mmu- HL miR-296-5p/rno-miR- 296*
hsa-miR-17*/rno-miR- HL 17-3p hsa-miR-452 HL hsa-miR-943 HL
hsa-miR-326/mmu-miR- HL 326/rno-miR-326 hsa-miR-652/mmu-miR- HL
652/rno-miR-652 hsa-miR-623 HL hsa-miR-194* HL hsa-miR-557 HL
hsa-miR-125a-3p/mmu- HL miR-125a-3p/rno-miR- 125a-3p
hsa-miR-425*/mmu- HL miR-425* hsa-miR-10a*/mmu- HL
miR-10a*/rno-miR-10a- 3p hsa-miR-323-3p/mmu- HL miR-323-3p/rno-miR-
323 hsa-miR-519e HL hsa-miR-502-5p HL hsa-miR-124*/mmu- HL
miR-124*/rno-miR-124* hsa-miR-345 HL hsa-miR-584 HL hsa-miR-654-5p
HL hsa-miR-331-5p/mmu- HL miR-331-5p hsa-miR-650 HL hsa-miR-202 HL
hsa-miR-548b-3p HL hsa-miR-492 HL hsa-miR-135a*/mmu- HL miR-135a*
ebv-miR-BART20-3p HL hsa-miR-586 HL hsa-miR-338-5p/mmu- HL
miR-338-5p/rno-miR- 338* hsa-miR-92b* HL hiv1-miR-H1 HL
hsa-miR-508-5p HL hsa-miR-542-5p/mmu- HL miR-542-5p/rno-miR- 542-5p
hsa-miR-490-5p HL hsa-miR-663 HL hsa-miR-433/mmu-miR- HL
433/rno-miR-433
Sequence CWU 1
1
1565122RNAhomo sapiens 1cugcgcaagc uacugccuug cu 22222RNAhomo
sapiens 2ccacggaugu uugagcaugu gc 22322RNAhomo sapiens 3ccacggaugu
uugagcaugu gc 22422RNAhomo sapiens 4uacugcaaug uaagcacuuc uu
22521RNAhomo sapiens 5cauuauuacu uuugguacgc g 21622RNAhomo sapiens
6aagcccuuac cccaaaaagu au 22722RNAhomo sapiens 7aagcccuuac
cccaaaaagc au 22822RNAhomo sapiens 8ucuacagugc acgugucucc ag
22922RNAhomo sapiens 9cugguacagg ccugggggac ag 221022RNAhomo
sapiens 10acugcaguga aggcacuugu ag 221121RNAhomo sapiens
11ugaauuaccg aagggccaua a 211222RNAhomo sapiens 12aggggcuggc
uuuccucugg uc 221323RNAhomo sapiens 13acugcccuaa gugcuccuuc ugg
231422RNAhomo sapiens 14aacuggccua caaaguccca gu 221522RNAhomo
sapiens 15cgggguuuug agggcgagau ga 221623RNAhomo sapiens
16cccaguguuc agacuaccug uuc 231722RNAhomo sapiens 17uaguuuugca
uaguugcacu ac 221823RNAhomo sapiens 18aguuuugcag guuugcaucc agc
231922RNAhomo sapiens 19acuguaguau gggcacuucc ag 222022RNAhomo
sapiens 20accuggcaua caauguagau uu 222121RNAhomo sapiens
21ggguuccugg caugcugauu u 212221RNAhomo sapiens 22aggcggagac
uugggcaauu g 212322RNAhomo sapiens 23agagcuuagc ugauugguga ac
222422RNAhomo sapiens 24cugggaggug gauguuuacu uc 222522RNAhomo
sapiens 25cugggagagg guuguuuacu cc 222622RNAhomo sapiens
26cugggagaag gcuguuuacu cu 222722RNAhomo sapiens 27cuuucagucg
gauguuuaca gc 222823RNAhomo sapiens 28ucccuguccu ccaggagcuc acg
232923RNAhomo sapiens 29ucucacacag aaaucgcacc cgu 233022RNAhomo
sapiens 30aaucagcaag uauacugccc ua 223123RNAhomo sapiens
31ucccccaggu gugauucuga uuu 233224RNAhomo sapiens 32aauccuugga
accuaggugu gagu 243322RNAhomo sapiens 33agggacuuuc aggggcagcu gu
223422RNAhomo sapiens 34cuuaucagau uguauuguaa uu 223522RNAhomo
sapiens 35cuuagcaggu uguauuauca uu 223623RNAhomo sapiens
36agguuacccg agcaacuuug cau 233721RNAhomo sapiens 37caaaacguga
ggcgcugcua u 213822RNAhomo sapiens 38uaugugccuu uggacuacau cg
223922RNAhomo sapiens 39gucauacacg gcucuccucu cu 224022RNAhomo
sapiens 40ugucuuacuc ccucaggcac au 224122RNAhomo sapiens
41ugucuuacuc ccucaggcac au 224222RNAhomo sapiens 42gaaaucaagc
gugggugaga cc 224322RNAhomo sapiens 43auucuaauuu cuccacgucu uu
224424RNAhomo sapiens 44ucagaacaaa ugccgguucc caga 244522RNAhomo
sapiens 45gagcuuauuc auaaaagugc ag 224622RNAhomo sapiens
46gggggucccc ggugcucgga uc 224722RNAhomo sapiens 47gacuauagaa
cuuucccccu ca 224822RNAhomo sapiens 48uggugggccg cagaacaugu gc
224923RNAhomo sapiens 49aggaagcccu ggaggggcug gag 235022RNAhomo
sapiens 50aacuagacug ugagcuucua ga 225122RNAhomo sapiens
51caacaaauca cagucugcca ua 225222RNAhomo sapiens 52auaaagcuag
auaaccgaaa gu 225322RNAhomo sapiens 53auaaagcuag auaaccgaaa gu
225422RNAhomo sapiens 54agggacggga cgcggugcag ug 225522RNAhomo
sapiens 55auaaagcuag auaaccgaaa gu 225622RNAhomo sapiens
56ugagguagua gguuguauag uu 225722RNAhomo sapiens 57ugagguagua
gguuguauag uu 225822RNAhomo sapiens 58ugagguagua gguuguaugg uu
225922RNAhomo sapiens 59ugagguagga gguuguauag uu 226022RNAhomo
sapiens 60ugagguagua gauuguauag uu 226122RNAhomo sapiens
61ugagguagua guuuguacag uu 226222RNAhomo sapiens 62ugagguagua
guuugugcug uu 226322RNAhomo sapiens 63aacccguaga uccgaacuug ug
226421RNAhomo sapiens 64uacaguacug ugauaacuga a 216522RNAhomo
sapiens 65guacaguacu gugauaacug aa 226623RNAhomo sapiens
66agcagcauug uacagggcua uga 236723RNAhomo sapiens 67agcagcauug
uacagggcua uga 236822RNAhomo sapiens 68aaaugcucag acuccugugg ug
226922RNAhomo sapiens 69aaaugcucag acuccugugg ug 227023RNAhomo
sapiens 70aaaagugcuu acagugcagg uag 237121RNAhomo sapiens
71uaaagugcug acagugcaga u 217223RNAhomo sapiens 72agcagcauug
uacagggcua uca 237323RNAhomo sapiens 73uacccuguag auccgaauuu gug
237423RNAhomo sapiens 74uacccuguag aaccgaauuu gug 237522RNAhomo
sapiens 75uggaauguaa agaaguaugu au 227622RNAhomo sapiens
76uggaauguaa agaaguaugu au 227722RNAhomo sapiens 77uggaguguga
caaugguguu ug 227820RNAhomo sapiens 78uaaggcacgc ggugaaugcc
207920RNAhomo sapiens 79uaaggcacgc ggugaaugcc 208020RNAhomo sapiens
80uaaggcacgc ggugaaugcc 208122RNAhomo sapiens 81acccgucccg
uucguccccg ga 228221RNAhomo sapiens 82acggugcugg auguggccuu u
218322RNAhomo sapiens 83agaaggaaau ugaauucauu ua 228424RNAhomo
sapiens 84agccuggaag cuggagccug cagu 248523RNAhomo sapiens
85aggaugagca aagaaaguag auu 238622RNAhomo sapiens 86aggcauugac
uucucacuag cu 228721RNAhomo sapiens 87cguaccguga guaauaaugc g
218822RNAhomo sapiens 88augggugaau uuguagaagg au 228922RNAhomo
sapiens 89augguacccu ggcauacuga gu 229022RNAhomo sapiens
90caggaugugg ucaaguguug uu 229123RNAhomo sapiens 91ccucagggcu
guagaacagg gcu 239222RNAhomo sapiens 92cuggacugag ccgugcuacu gg
229323RNAhomo sapiens 93cuggagauau ggaagagcug ugu 239422RNAhomo
sapiens 94cuuggcaccu agcaagcacu ca 229522RNAhomo sapiens
95ucggauccgu cugagcuugg cu 229622RNAhomo sapiens 96uacguagaua
uauauguauu uu 229722RNAhomo sapiens 97uaguacugug cauaucaucu au
229821RNAhomo sapiens 98ucacagugaa ccggucucuu u 219921RNAhomo
sapiens 99ucacagugaa ccggucucuu u 2110021RNAhomo sapiens
100cuuuuugcgg ucugggcuug c 2110121RNAhomo sapiens 101cuuuuugcgg
ucugggcuug c 2110222RNAhomo sapiens 102ugugagguug gcauuguugu cu
2210321RNAhomo sapiens 103uuaggccgca gaucugggug a 2110422RNAhomo
sapiens 104uucauucggc uguccagaug ua 2210524RNAhomo sapiens
105uugcagcugc cugggaguga cuuc 2410622RNAhomo sapiens 106cgguuugagg
cuacagugag au 2210718RNAhomo sapiens 107acguuggcuc ugguggug
1810822RNAhomo sapiens 108acucggcgug gcgucggucg ug 2210922RNAhomo
sapiens 109cagugcaaug uuaaaagggc au 2211022RNAhomo sapiens
110cagugcaaug augaaagggc au 2211122RNAhomo sapiens 111uaacagucua
cagccauggu cg 2211222RNAhomo sapiens 112accguggcuu ucgauuguua cu
2211322RNAhomo sapiens 113ugugacuggu ugaccagagg gg 2211423RNAhomo
sapiens 114uauggcuuuu cauuccuaug uga 2311523RNAhomo sapiens
115acuccauuug uuuugaugau gga 2311623RNAhomo sapiens 116agcugguguu
gugaaucagg ccg 2311723RNAhomo sapiens 117agcugguguu gugaaucagg ccg
2311822RNAhomo sapiens 118uggagacgcg gcccuguugg ag 2211921RNAhomo
sapiens 119uaccacaggg uagaaccacg g 2112022RNAhomo sapiens
120uaacacuguc ugguaaagau gg 2212120RNAhomo sapiens 121cccauaaagu
agaaagcacu 2012221RNAhomo sapiens 122ugagaugaag cacuguagcu c
2112322RNAhomo sapiens 123ggauaucauc auauacugua ag 2212423RNAhomo
sapiens 124guccaguuuu cccaggaauc ccu 2312522RNAhomo sapiens
125ugagaacuga auuccauggg uu 2212622RNAhomo sapiens 126ugagaacuga
auuccauagg cu 2212722RNAhomo sapiens 127ucagugcacu acagaacuuu gu
2212822RNAhomo sapiens 128ucagugcauc acagaacuuu gu 2212922RNAhomo
sapiens 129ucucccaacc cuuguaccag ug 2213021RNAhomo sapiens
130cuagacugaa gcuccuugag g 2113121RNAhomo sapiens 131ucagugcaug
acagaacuug g 2113223RNAhomo sapiens 132uuaaugcuaa ucgugauagg ggu
2313322RNAhomo sapiens 133uagcagcaca uaaugguuug ug 2213422RNAhomo
sapiens 134uagcagcaca ucaugguuua ca 2213522RNAhomo sapiens
135uagcagcacg uaaauauugg cg 2213622RNAhomo sapiens 136uagcagcacg
uaaauauugg cg 2213723RNAhomo sapiens 137caaagugcuu acagugcagg uag
2313823RNAhomo sapiens 138aacauucaac gcugucggug agu 2313923RNAhomo
sapiens 139aacauucaac gcugucggug agu 2314023RNAhomo sapiens
140aacauucauu gcugucggug ggu 2314123RNAhomo sapiens 141aacauucauu
gcugucggug ggu 2314222RNAhomo sapiens 142aacauucaac cugucgguga gu
2214323RNAhomo sapiens 143aacauucauu guugucggug ggu 2314424RNAhomo
sapiens 144uuuggcaaug guagaacuca cacu 2414522RNAhomo sapiens
145uauggcacug guagaauuca cu 2214622RNAhomo sapiens 146uggacggaga
acugauaagg gu 2214722RNAhomo sapiens 147uggagagaaa ggcaguuccu ga
2214822RNAhomo sapiens 148caaagaauuc uccuuuuggg cu 2214921RNAhomo
sapiens 149caucccuugc augguggagg g 2115023RNAhomo sapiens
150uaaggugcau cuagugcaga uag 2315123RNAhomo sapiens 151caacggaauc
ccaaaagcag cug 2315221RNAhomo sapiens 152cugaccuaug aauugacagc c
2115322RNAhomo sapiens 153ugggucuuug cgggcgagau ga 2215422RNAhomo
sapiens 154aacuggcccu caaagucccg cu 2215522RNAhomo sapiens
155uguaacagca acuccaugug ga 2215621RNAhomo sapiens 156uagcagcaca
gaaauauugg c 2115722RNAhomo sapiens 157uagguaguuu cauguuguug gg
2215822RNAhomo sapiens 158uagguaguuu cauguuguug gg 2215922RNAhomo
sapiens 159uagguaguuu ccuguuguug gg 2216022RNAhomo sapiens
160uucaccaccu ucuccaccca gc 2216122RNAhomo sapiens 161acaguagucu
gcacauuggu ua 2216222RNAhomo sapiens 162acaguagucu gcacauuggu ua
2216322RNAhomo sapiens 163acaguagucu gcacauuggu ua 2216423RNAhomo
sapiens 164ugugcaaauc uaugcaaaac uga 2316523RNAhomo sapiens
165ugugcaaauc caugcaaaac uga 2316623RNAhomo sapiens 166ugugcaaauc
caugcaaaac uga 2316722RNAhomo sapiens 167uaacacuguc ugguaacgau gu
2216822RNAhomo sapiens 168uaauacugcc ugguaaugau ga 2216923RNAhomo
sapiens 169uaauacugcc ggguaaugau gga 2317022RNAhomo sapiens
170uuccuaugca uauacuucuu ug 2217122RNAhomo sapiens 171gugaaauguu
uaggaccacu ag 2217222RNAhomo sapiens 172uucccuuugu cauccuaugc cu
2217322RNAhomo sapiens 173uccuucauuc caccggaguc ug 2217422RNAhomo
sapiens 174uggaauguaa ggaagugugu gg 2217523RNAhomo sapiens
175uaaagugcuu auagugcagg uag 2317623RNAhomo sapiens 176caaagugcuc
auagugcagg uag 2317722RNAhomo sapiens 177uagcuuauca gacugauguu ga
2217822RNAhomo sapiens 178cugugcgugu gacagcggcu ga 2217921RNAhomo
sapiens 179uaacagucuc cagucacggc c 2118022RNAhomo sapiens
180acagcaggca cagacaggca gu 2218118RNAhomo sapiens 181ugaccuauga
auugacag 1818222RNAhomo sapiens 182aaaucucugc aggcaaaugu ga
2218322RNAhomo sapiens 183auacugcauc aggaacugau ug 2218422RNAhomo
sapiens 184agaguugagu cuggacgucc cg 2218522RNAhomo sapiens
185aagcugccag uugaagaacu gu 2218623RNAhomo sapiens 186agcuacauug
ucugcugggu uuc 2318721RNAhomo sapiens 187agcuacaucu ggcuacuggg u
2118822RNAhomo sapiens 188cguguauuug acaagcugag uu 2218921RNAhomo
sapiens 189caagucacua gugguuccgu u
2119021RNAhomo sapiens 190aucacauugc cagggauuuc c 2119121RNAhomo
sapiens 191aucacauugc cagggauuac c 2119222RNAhomo sapiens
192uggcucaguu cagcaggaac ag 2219322RNAhomo sapiens 193uggcucaguu
cagcaggaac ag 2219422RNAhomo sapiens 194cauugcacuu gucucggucu ga
2219522RNAhomo sapiens 195uucaaguaau ccaggauagg cu 2219622RNAhomo
sapiens 196uucaaguaau ccaggauagg cu 2219721RNAhomo sapiens
197uucaaguaau ucaggauagg u 2119821RNAhomo sapiens 198uucacagugg
cuaaguuccg c 2119921RNAhomo sapiens 199uucacagugg cuaaguucug c
2120022RNAhomo sapiens 200cacuagauug ugagcuccug ga 2220122RNAhomo
sapiens 201gaggguuggg uggaggcucu cc 2220222RNAhomo sapiens
202augguuuacc gucccacaua ca 2220322RNAhomo sapiens 203uagcaccauc
ugaaaucggu ua 2220423RNAhomo sapiens 204uagcaccauu ugaaaucagu guu
2320523RNAhomo sapiens 205uagcaccauu ugaaaucagu guu 2320622RNAhomo
sapiens 206uagcaccauu ugaaaucggu ua 2220723RNAhomo sapiens
207cagugcaaug auauugucaa agc 2320822RNAhomo sapiens 208uguaaacauc
cucgacugga ag 2220922RNAhomo sapiens 209uguaaacauc cuacacucag cu
2221023RNAhomo sapiens 210uguaaacauc cuacacucuc agc 2321123RNAhomo
sapiens 211uguaaacauc cuacacucuc agc 2321222RNAhomo sapiens
212uguaaacauc cccgacugga ag 2221322RNAhomo sapiens 213uguaaacauc
cuugacugga ag 2221421RNAhomo sapiens 214aggcaagaug cuggcauagc u
2121522RNAhomo sapiens 215uauugcacau uacuaaguug ca 2221622RNAhomo
sapiens 216aaaagcuggg uugagagggc ga 2221722RNAhomo sapiens
217aaaagcuggg uugagagggc aa 2221822RNAhomo sapiens 218aaaagcuggg
uugagagggc aa 2221920RNAhomo sapiens 219aaaagcuggg uugagagggu
2022020RNAhomo sapiens 220aaaagcuggg uugagagggu 2022119RNAhomo
sapiens 221aaaagcuggg uugagagga 1922219RNAhomo sapiens
222aaaagcuggg uugagagga 1922321RNAhomo sapiens 223cacauuacac
ggucgaccuc u 2122423RNAhomo sapiens 224cgcauccccu agggcauugg ugu
2322522RNAhomo sapiens 225cuggcccucu cugcccuucc gu 2222623RNAhomo
sapiens 226gcaaagcaca cggccugcag aga 2322721RNAhomo sapiens
227gccccugggc cuauccuaga a 2122823RNAhomo sapiens 228ucaagagcaa
uaacgaaaaa ugu 2322922RNAhomo sapiens 229uccagcauca gugauuuugu ug
2223023RNAhomo sapiens 230ugagcgccuc gacgacagag ccg 2323121RNAhomo
sapiens 231gugcauugua guugcauugc a 2123220RNAhomo sapiens
232gugcauugcu guugcauugc 2023322RNAhomo sapiens 233uuauaaagca
augagacuga uu 2223421RNAhomo sapiens 234aggggugcua ucugugauug a
2123522RNAhomo sapiens 235gcugacuccu aguccagggc uc 2223622RNAhomo
sapiens 236uggcaguguc uuagcugguu gu 2223722RNAhomo sapiens
237aggcaguguc auuagcugau ug 2223823RNAhomo sapiens 238aggcagugua
guuagcugau ugc 2323922RNAhomo sapiens 239uuaucagaau cuccaggggu ac
2224022RNAhomo sapiens 240aacacaccua uucaaggauu ca 2224122RNAhomo
sapiens 241aauugcacgg uauccaucug ua 2224222RNAhomo sapiens
242uaaugccccu aaaaauccuu au 2224321RNAhomo sapiens 243aauaauacau
gguugaucuu u 2124422RNAhomo sapiens 244gccugcuggg guggaaccug gu
2224520RNAhomo sapiens 245acucaaacug ugggggcacu 2024622RNAhomo
sapiens 246uuauaauaca accugauaag ug 2224722RNAhomo sapiens
247auauaauaca accugcuaag ug 2224822RNAhomo sapiens 248uuuguucguu
cggcucgcgu ga 2224922RNAhomo sapiens 249agagguugcc cuuggugaau uc
2225021RNAhomo sapiens 250acuggacuug gagucagaag g 2125121RNAhomo
sapiens 251ugguagacua uggaacguag g 2125222RNAhomo sapiens
252uauacaaggg caagcucucu gu 2225322RNAhomo sapiens 253gaaguuguuc
gugguggauu cg 2225422RNAhomo sapiens 254agaucagaag gugauugugg cu
2225522RNAhomo sapiens 255cgaauguugc ucggugaacc cc 2225621RNAhomo
sapiens 256aauauaacac agauggccug u 2125722RNAhomo sapiens
257auaguagacc guauagcgua cg 2225823RNAhomo sapiens 258aucaacagac
auuaauuggg cgc 2325923RNAhomo sapiens 259ugaggggcag agagcgagac uuu
2326022RNAhomo sapiens 260cagcagcaau ucauguuuug aa 2226122RNAhomo
sapiens 261uaauacuguc ugguaaaacc gu 2226223RNAhomo sapiens
262ucuuggagua ggucauuggg ugg 2326322RNAhomo sapiens 263aucaugaugg
gcuccucggu gu 2226422RNAhomo sapiens 264uugcauaugu aggauguccc au
2226522RNAhomo sapiens 265uggcagugua uuguuagcug gu 2226622RNAhomo
sapiens 266aggcagugua uuguuagcug gc 2226722RNAhomo sapiens
267uuuugcgaug uguuccuaau au 2226822RNAhomo sapiens 268uuuugcgaug
uguuccuaau au 2226922RNAhomo sapiens 269uuuugcaaua uguuccugaa ua
2227022RNAhomo sapiens 270aacuguuugc agaggaaacu ga 2227123RNAhomo
sapiens 271uagugcaaua uugcuuauag ggu 2327221RNAhomo sapiens
272gcaguccaug ggcauauaca c 2127322RNAhomo sapiens 273aagacgggag
gaaagaaggg ag 2227422RNAhomo sapiens 274ucaggcucag uccccucccg au
2227522RNAhomo sapiens 275agaggcuggc cgugaugaau uc 2227622RNAhomo
sapiens 276uccuguacug agcugccccg ag 2227722RNAhomo sapiens
277aaucguacag ggucauccac uu 2227821RNAhomo sapiens 278cccagauaau
ggcacucuca a 2127922RNAhomo sapiens 279uuguacaugg uaggcuuuca uu
2228022RNAhomo sapiens 280ugaaacauac acgggaaacc uc 2228122RNAhomo
sapiens 281aaacaaacau ggugcacuuc uu 2228221RNAhomo sapiens
282cagcagcaca cugugguuug u 2128321RNAhomo sapiens 283uuaagacuug
cagugauguu u 2128422RNAhomo sapiens 284augcaccugg gcaaggauuc ug
2228522RNAhomo sapiens 285aaugcacccg ggcaaggauu cu 2228622RNAhomo
sapiens 286aaugcaccug ggcaaggauu ca 2228723RNAhomo sapiens
287uagcagcggg aacaguucug cag 2328822RNAhomo sapiens 288agacccuggu
cugcacucua uc 2228922RNAhomo sapiens 289gggagccagg aaguauugau gu
2229023RNAhomo sapiens 290ugauuguagc cuuuuggagu aga 2329122RNAhomo
sapiens 291uacugcagac guggcaauca ug 2229218RNAhomo sapiens
292uucacaggga ggugucau 1829318RNAhomo sapiens 293uucacaggga
ggugucau 1829422RNAhomo sapiens 294uucacaagga ggugucauuu au
2229522RNAhomo sapiens 295uucucaagga ggugucguuu au 2229622RNAhomo
sapiens 296caugccuuga guguaggacc gu 2229722RNAhomo sapiens
297ugugacagau ugauaacuga aa 2229822RNAhomo sapiens 298aaacauucgc
ggugcacuuc uu 2229922RNAhomo sapiens 299caaaacuggc aauuacuuuu gc
2230022RNAhomo sapiens 300caaaacuggc aauuacuuuu gc 2230122RNAhomo
sapiens 301caaaacuggc aauuacuuuu gc 2230222RNAhomo sapiens
302caagaaccuc aguugcuuuu gu 2230322RNAhomo sapiens 303aaaaacugag
acuacuuuug ca 2230422RNAhomo sapiens 304aaaaguaauc gcgguuuuug uc
2230522RNAhomo sapiens 305aaaaguaauc gcgguuuuug uc 2230622RNAhomo
sapiens 306aaaaguaauc gcgguuuuug uc 2230722RNAhomo sapiens
307aaaaguaauc gcgguuuuug uc 2230822RNAhomo sapiens 308aaaaguaauu
gcggucuuug gu 2230922RNAhomo sapiens 309aaaaguacuu gcggauuuug cu
2231022RNAhomo sapiens 310aaaaguauuu gcggguuuug uc 2231122RNAhomo
sapiens 311caaaaguaau uguggauuuu gu 2231223RNAhomo sapiens
312agugccugag ggaguaagag ccc 2331323RNAhomo sapiens 313agugccugag
ggaguaagag ccc 2331421RNAhomo sapiens 314gcgacccaua cuugguuuca g
2131522RNAhomo sapiens 315cacgcucaug cacacaccca ca 2231622RNAhomo
sapiens 316aagaugugga aaaauuggaa uc 2231722RNAhomo sapiens
317guagauaaaa uauugguacc ug 2231822RNAhomo sapiens 318uaacugguug
aacaacugaa cc 2231923RNAhomo sapiens 319uuacaguugu ucaaccaguu acu
2332022RNAhomo sapiens 320uuaugguuug ccugggacug ag 2232122RNAhomo
sapiens 321ugagaaccac gucugcucug ag 2232221RNAhomo sapiens
322uaauuuuaug uauaagcuag u 2132322RNAhomo sapiens 323uacgucaucg
uugucaucgu ca 2232422RNAhomo sapiens 324uccgagccug ggucucccuc uu
2232522RNAhomo sapiens 325aagucauugg aggguuugag ca 2232623RNAhomo
sapiens 326aaacucuacu uguccuucug agu 2332721RNAhomo sapiens
327agggggaaag uucuauaguc c 2132822RNAhomo sapiens 328augcugacau
auuuacuaga gg 2232921RNAhomo sapiens 329uggguuuacg uugggagaac u
2133024RNAhomo sapiens 330aaagacauag gauagaguca ccuc 2433122RNAhomo
sapiens 331acacuuguau gcuagcucag gu 2233222RNAhomo sapiens
332uuuaggauaa gcuugacuuu ug 2233321RNAhomo sapiens 333aauggcgcca
cuaggguugu g 2133422RNAhomo sapiens 334uaugucugcu gaccaucacc uu
2233522RNAhomo sapiens 335uacccauugc auaucggagu ug 2233621RNAhomo
sapiens 336uccgguucuc agggcuccac c 2133723RNAhomo sapiens
337aaggagcuua caaucuagcu ggg 2333823RNAhomo sapiens 338uggaagacua
gugauuuugu ugu 2333923RNAhomo sapiens 339uggaagacua gugauuuugu ugu
2334023RNAhomo sapiens 340uggaagacua gugauuuugu ugu 2334120RNAhomo
sapiens 341cggcucuggg ucugugggga 2034223RNAhomo sapiens
342ugcaccaugg uugucugagc aug 2334322RNAhomo sapiens 343ugagaccucu
ggguucugag cu 2234421RNAhomo sapiens 344gcaggaacuu gugagucucc u
2134522RNAhomo sapiens 345cugcccuggc ccgagggacc ga 2234620RNAhomo
sapiens 346guagaggaga uggcgcaggg 2034722RNAhomo sapiens
347gugaacgggc gccaucccga gg 2234821RNAhomo sapiens 348uuaauaucgg
acaaccauug u 2134922RNAhomo sapiens 349ugcaacgaac cugagccacu ga
2235021RNAhomo sapiens 350cacugugucc uuucugcgua g 2135123RNAhomo
sapiens 351ucuuugguua ucuagcugua uga 2335223RNAhomo sapiens
352ucuuugguua ucuagcugua uga 2335322RNAhomo sapiens 353uauugcacuu
gucccggccu gu 2235422RNAhomo sapiens 354uauugcacuu gucccggccu gu
2235522RNAhomo sapiens 355uauugcacuc gucccggccu cc 2235623RNAhomo
sapiens 356caaagugcug uucgugcagg uag 2335723RNAhomo sapiens
357ucuuugguua ucuagcugua uga 2335821RNAhomo sapiens 358aaggcagggc
ccccgcuccc c 2135922RNAhomo sapiens 359uucucuguuu uggccaugug ug
2236022RNAhomo sapiens 360aaauuauugu acaucggaug ag 2236122RNAhomo
sapiens 361uucaacgggu auuuauugag ca 2236223RNAhomo sapiens
362uuuggcacua gcacauuuuu gcu 2336322RNAhomo sapiens 363ugagguagua
aguuguauug uu 2236422RNAhomo sapiens 364aacccguaga uccgaucuug ug
2236522RNAhomo sapiens 365cacccguaga accgaccuug cg 2236621RNAhomo
sapiens 366aacauagagg aaauuccacg u 2136722RNAhomo sapiens
367aucauagagg aaaauccaug uu 2236822RNAhomo sapiens 368uggugggccg
cagaacaugu gc 2236921RNAhomo sapiens 369aucauagagg aaaauccacg u
2137021RNAhomo sapiens 370aucauagagg aaaauccacg u 2137122RNAhomo
sapiens 371aaaaccgucu aguuacaguu gu 2237222RNAhomo sapiens
372auauacaggg ggagacucuc au 2237320RNAhomo sapiens 373ccuagaaugg
ggauuguggg 2037422RNAhomo sapiens 374agcgagaccu caacucuaca au
2237522RNAhomo sapiens 375ucgaccggac cucgaccggc uc 2237622RNAhomo
sapiens 376auguagggau ggaagccaug aa 2237721RNAhomo sapiens
377cucacugaac aaugaaugca a
2137822RNAhomo sapiens 378cggguagaga gggcaguggg ag 2237921RNAhomo
sapiens 379gcugggaagg caaagggacg u 2138022RNAhomo sapiens
380accuuggcuc uagacugcuu ac 2238122RNAhomo sapiens 381gcucugacuu
uauugcacua cu 2238222RNAhomo sapiens 382agggacuuuu gggggcagau gu
2238321RNAhomo sapiens 383uuggggacau uuugcauuca u 2138420RNAhomo
sapiens 384aauguguagc aaaagacaga 2038520RNAhomo sapiens
385aauguguagc aaaagacaga 2038622RNAhomo sapiens 386aucauacaag
gacaauuucu uu 2238722RNAhomo sapiens 387aaagguaauu gcaguuuuuc cc
2238822RNAhomo sapiens 388ucgcgguuug ugccagauga cg 2238922RNAhomo
sapiens 389agaaggcacu augagauuua ga 2239022RNAhomo sapiens
390agacacauuu ggagagggaa cc 2239122RNAhomo sapiens 391aggaccuucc
cugaaccaag ga 2239222RNAhomo sapiens 392auauacaggg ggagacucuu au
2239322RNAhomo sapiens 393cugcgcaagc uacugccuug cu 2239422RNAhomo
sapiens 394acggauguuu gagcaugugc ua 2239522RNAhomo sapiens
395acggauguuu gagcaugugc ua 2239622RNAhomo sapiens 396cugcaaugua
agcacuucuu ac 2239721RNAhomo sapiens 397cauuauuacu uuugguacgc g
2139822RNAhomo sapiens 398aagcccuuac cccaaaaagu au 2239922RNAhomo
sapiens 399aagcccuuac cccaaaaagc au 2240022RNAhomo sapiens
400ucuacagugc acgugucucc ag 2240122RNAhomo sapiens 401cugguacagg
ccugggggac ag 2240222RNAhomo sapiens 402acugcaguga aggcacuugu ag
2240322RNAhomo sapiens 403gugaauuacc gaagggccau aa 2240422RNAhomo
sapiens 404aggggcuggc uuuccucugg uc 2240523RNAhomo sapiens
405acugcccuaa gugcuccuuc ugg 2340622RNAhomo sapiens 406aacuggccua
caaaguccca gu 2240722RNAhomo sapiens 407cgggguuuug agggcgagau ga
2240823RNAhomo sapiens 408cccaguguuc agacuaccug uuc 2340922RNAhomo
sapiens 409aguuuugcau aguugcacua ca 2241023RNAhomo sapiens
410aguuuugcag guuugcaucc agc 2341122RNAhomo sapiens 411acuguaguau
gggcacuucc ag 2241222RNAhomo sapiens 412accuggcaua caauguagau uu
2241322RNAhomo sapiens 413uggguuccug gcaugcugau uu 2241421RNAhomo
sapiens 414aggcggagac uugggcaauu g 2141522RNAhomo sapiens
415agagcuuagc ugauugguga ac 2241622RNAhomo sapiens 416cugggaggug
gauguuuacu uc 2241722RNAhomo sapiens 417cugggagagg guuguuuacu cc
2241822RNAhomo sapiens 418cugggagaag gcuguuuacu cu 2241922RNAhomo
sapiens 419cuuucagucg gauguuuaca gc 2242023RNAhomo sapiens
420ucccuguccu ccaggagcuc acg 2342123RNAhomo sapiens 421ucucacacag
aaaucgcacc cgu 2342222RNAhomo sapiens 422caaucagcaa guauacugcc cu
2242323RNAhomo sapiens 423ucccccaggu gugauucuga uuu 2342424RNAhomo
sapiens 424aauccuugga accuaggugu gagu 2442522RNAhomo sapiens
425agggacuuuc aggggcagcu gu 2242622RNAhomo sapiens 426cuuaucagau
uguauuguaa uu 2242722RNAhomo sapiens 427cuuagcaggu uguauuauca uu
2242823RNAhomo sapiens 428agguuacccg agcaacuuug cau 2342921RNAhomo
sapiens 429caaaacguga ggcgcugcua u 2143022RNAhomo sapiens
430uaugugccuu uggacuacau cg 2243122RNAhomo sapiens 431gucauacacg
gcucuccucu cu 2243222RNAhomo sapiens 432ugucuuacuc ccucaggcac au
2243322RNAhomo sapiens 433ugucuuacuc ccucaggcac au 2243422RNAhomo
sapiens 434gaaaucaagc gugggugaga cc 2243522RNAhomo sapiens
435auucuaauuu cuccacgucu uu 2243624RNAhomo sapiens 436ucagaacaaa
ugccgguucc caga 2443722RNAhomo sapiens 437gagcuuauuc auaaaagugc ag
2243822RNAhomo sapiens 438gggggucccc ggugcucgga uc 2243922RNAhomo
sapiens 439gacuauagaa cuuucccccu ca 2244022RNAhomo sapiens
440uggugggccg cagaacaugu gc 2244123RNAhomo sapiens 441aggaagcccu
ggaggggcug gag 2344222RNAhomo sapiens 442caacuagacu gugagcuucu ag
2244322RNAhomo sapiens 443caacaaauca cagucugcca ua 2244422RNAhomo
sapiens 444auaaagcuag auaaccgaaa gu 2244522RNAhomo sapiens
445auaaagcuag auaaccgaaa gu 2244622RNAhomo sapiens 446agggacggga
cgcggugcag ug 2244722RNAhomo sapiens 447auaaagcuag auaaccgaaa gu
2244822RNAhomo sapiens 448ugagguagua gguuguauag uu 2244922RNAhomo
sapiens 449ugagguagua gguuguauag uu 2245022RNAhomo sapiens
450ugagguagua gguuguaugg uu 2245122RNAhomo sapiens 451ugagguagga
gguuguauag uu 2245222RNAhomo sapiens 452ugagguagua gauuguauag uu
2245322RNAhomo sapiens 453ugagguagua guuuguacag uu 2245422RNAhomo
sapiens 454ugagguagua guuugugcug uu 2245522RNAhomo sapiens
455aacccguaga uccgaacuug ug 2245621RNAhomo sapiens 456uacaguacug
ugauaacuga a 2145721RNAhomo sapiens 457uacaguacug ugauaacuga a
2145823RNAhomo sapiens 458agcagcauug uacagggcua uga 2345923RNAhomo
sapiens 459agcagcauug uacagggcua uga 2346023RNAhomo sapiens
460ucaaaugcuc agacuccugu ggu 2346123RNAhomo sapiens 461ucaaaugcuc
agacuccugu ggu 2346223RNAhomo sapiens 462aaaagugcuu acagugcagg uag
2346321RNAhomo sapiens 463uaaagugcug acagugcaga u 2146423RNAhomo
sapiens 464agcagcauug uacagggcua uca 2346523RNAhomo sapiens
465uacccuguag auccgaauuu gug 2346623RNAhomo sapiens 466uacccuguag
aaccgaauuu gug 2346722RNAhomo sapiens 467uggaauguaa agaaguaugu au
2246822RNAhomo sapiens 468uggaauguaa agaaguaugu au 2246922RNAhomo
sapiens 469uggaguguga caaugguguu ug 2247020RNAhomo sapiens
470uaaggcacgc ggugaaugcc 2047120RNAhomo sapiens 471uaaggcacgc
ggugaaugcc 2047220RNAhomo sapiens 472uaaggcacgc ggugaaugcc
2047322RNAhomo sapiens 473acccgucccg uucguccccg ga 2247421RNAhomo
sapiens 474acggugcugg auguggccuu u 2147522RNAhomo sapiens
475agaaggaaau ugaauucauu ua 2247624RNAhomo sapiens 476agccuggaag
cuggagccug cagu 2447723RNAhomo sapiens 477aggaugagca aagaaaguag auu
2347822RNAhomo sapiens 478aggcauugac uucucacuag cu 2247922RNAhomo
sapiens 479ucguaccgug aguaauaaug cg 2248022RNAhomo sapiens
480augggugaau uuguagaagg au 2248122RNAhomo sapiens 481augguacccu
ggcauacuga gu 2248222RNAhomo sapiens 482caggaugugg ucaaguguug uu
2248323RNAhomo sapiens 483ccucagggcu guagaacagg gcu 2348422RNAhomo
sapiens 484cuggacugag ccgugcuacu gg 2248523RNAhomo sapiens
485cuggagauau ggaagagcug ugu 2348622RNAhomo sapiens 486cuuggcaccu
agcaagcacu ca 2248722RNAhomo sapiens 487ucggauccgu cugagcuugg cu
2248822RNAhomo sapiens 488uacguagaua uauauguauu uu 2248922RNAhomo
sapiens 489uaguacugug cauaucaucu au 2249021RNAhomo sapiens
490ucacagugaa ccggucucuu u 2149121RNAhomo sapiens 491ucacagugaa
ccggucucuu u 2149221RNAhomo sapiens 492cuuuuugcgg ucugggcuug c
2149321RNAhomo sapiens 493cuuuuugcgg ucugggcuug c 2149422RNAhomo
sapiens 494ugugagguug gcauuguugu cu 2249521RNAhomo sapiens
495uuaggccgca gaucugggug a 2149622RNAhomo sapiens 496uucauucggc
uguccagaug ua 2249724RNAhomo sapiens 497uugcagcugc cugggaguga cuuc
2449822RNAhomo sapiens 498uuugaggcua cagugagaug ug 2249918RNAhomo
sapiens 499acguuggcuc ugguggug 1850022RNAhomo sapiens 500acucggcgug
gcgucggucg ug 2250122RNAhomo sapiens 501cagugcaaug uuaaaagggc au
2250222RNAhomo sapiens 502cagugcaaug augaaagggc au 2250322RNAhomo
sapiens 503uaacagucua cagccauggu cg 2250422RNAhomo sapiens
504accguggcuu ucgauuguua cu 2250522RNAhomo sapiens 505ugugacuggu
ugaccagagg gg 2250623RNAhomo sapiens 506uauggcuuuu cauuccuaug uga
2350723RNAhomo sapiens 507acuccauuug uuuugaugau gga 2350823RNAhomo
sapiens 508agcugguguu gugaaucagg ccg 2350923RNAhomo sapiens
509agcugguguu gugaaucagg ccg 2351022RNAhomo sapiens 510ggagacgcgg
cccuguugga gu 2251121RNAhomo sapiens 511uaccacaggg uagaaccacg g
2151222RNAhomo sapiens 512uaacacuguc ugguaaagau gg 2251321RNAhomo
sapiens 513cauaaaguag aaagcacuac u 2151421RNAhomo sapiens
514ugagaugaag cacuguagcu c 2151522RNAhomo sapiens 515ggauaucauc
auauacugua ag 2251623RNAhomo sapiens 516guccaguuuu cccaggaauc ccu
2351722RNAhomo sapiens 517ugagaacuga auuccauggg uu 2251822RNAhomo
sapiens 518ugagaacuga auuccauagg cu 2251922RNAhomo sapiens
519ucagugcacu acagaacuuu gu 2252022RNAhomo sapiens 520ucagugcauc
acagaacuuu gu 2252122RNAhomo sapiens 521ucucccaacc cuuguaccag ug
2252221RNAhomo sapiens 522cuagacugaa gcuccuugag g 2152321RNAhomo
sapiens 523ucagugcaug acagaacuug g 2152423RNAhomo sapiens
524uuaaugcuaa ucgugauagg ggu 2352522RNAhomo sapiens 525uagcagcaca
uaaugguuug ug 2252622RNAhomo sapiens 526uagcagcaca ucaugguuua ca
2252722RNAhomo sapiens 527uagcagcacg uaaauauugg cg 2252822RNAhomo
sapiens 528uagcagcacg uaaauauugg cg 2252923RNAhomo sapiens
529caaagugcuu acagugcagg uag 2353023RNAhomo sapiens 530aacauucaac
gcugucggug agu 2353123RNAhomo sapiens 531aacauucaac gcugucggug agu
2353223RNAhomo sapiens 532aacauucauu gcugucggug ggu 2353323RNAhomo
sapiens 533aacauucauu gcugucggug ggu 2353422RNAhomo sapiens
534aacauucaac cugucgguga gu 2253523RNAhomo sapiens 535aacauucauu
guugucggug ggu 2353624RNAhomo sapiens 536uuuggcaaug guagaacuca cacu
2453722RNAhomo sapiens 537uauggcacug guagaauuca cu 2253822RNAhomo
sapiens 538uggacggaga acugauaagg gu 2253922RNAhomo sapiens
539uggagagaaa ggcaguuccu ga 2254022RNAhomo sapiens 540caaagaauuc
uccuuuuggg cu 2254121RNAhomo sapiens 541caucccuugc augguggagg g
2154223RNAhomo sapiens 542uaaggugcau cuagugcaga uag 2354323RNAhomo
sapiens 543caacggaauc ccaaaagcag cug 2354421RNAhomo sapiens
544cugaccuaug aauugacagc c 2154522RNAhomo sapiens 545ugggucuuug
cgggcgagau ga 2254622RNAhomo sapiens 546aacuggcccu caaagucccg cu
2254722RNAhomo sapiens 547uguaacagca acuccaugug ga 2254821RNAhomo
sapiens 548uagcagcaca gaaauauugg c 2154922RNAhomo sapiens
549uagguaguuu cauguuguug gg 2255022RNAhomo sapiens 550uagguaguuu
cauguuguug gg 2255122RNAhomo sapiens 551uagguaguuu ccuguuguug gg
2255222RNAhomo sapiens 552uucaccaccu ucuccaccca gc 2255322RNAhomo
sapiens 553acaguagucu gcacauuggu ua 2255422RNAhomo sapiens
554acaguagucu gcacauuggu ua 2255522RNAhomo sapiens 555acaguagucu
gcacauuggu ua 2255623RNAhomo sapiens 556ugugcaaauc uaugcaaaac uga
2355723RNAhomo sapiens 557ugugcaaauc caugcaaaac uga 2355823RNAhomo
sapiens 558ugugcaaauc caugcaaaac uga 2355922RNAhomo sapiens
559uaacacuguc ugguaacgau gu 2256022RNAhomo sapiens 560uaauacugcc
ugguaaugau ga 2256123RNAhomo sapiens 561uaauacugcc ggguaaugau gga
2356222RNAhomo sapiens 562uuccuaugca uauacuucuu ug 2256322RNAhomo
sapiens 563gugaaauguu uaggaccacu ag 2256422RNAhomo sapiens
564uucccuuugu cauccuaugc cu 2256522RNAhomo sapiens 565uccuucauuc
caccggaguc ug 2256622RNAhomo sapiens
566uggaauguaa ggaagugugu gg 2256723RNAhomo sapiens 567uaaagugcuu
auagugcagg uag 2356823RNAhomo sapiens 568caaagugcuc auagugcagg uag
2356922RNAhomo sapiens 569uagcuuauca gacugauguu ga 2257022RNAhomo
sapiens 570cugugcgugu gacagcggcu ga 2257121RNAhomo sapiens
571uaacagucuc cagucacggc c 2157222RNAhomo sapiens 572acagcaggca
cagacaggca gu 2257321RNAhomo sapiens 573augaccuaug aauugacaga c
2157422RNAhomo sapiens 574aaaucucugc aggcaaaugu ga 2257523RNAhomo
sapiens 575uacugcauca ggaacugauu gga 2357622RNAhomo sapiens
576agaguugagu cuggacgucc cg 2257722RNAhomo sapiens 577aagcugccag
uugaagaacu gu 2257823RNAhomo sapiens 578agcuacauug ucugcugggu uuc
2357921RNAhomo sapiens 579agcuacaucu ggcuacuggg u 2158022RNAhomo
sapiens 580cguguauuug acaagcugag uu 2258121RNAhomo sapiens
581caagucacua gugguuccgu u 2158221RNAhomo sapiens 582aucacauugc
cagggauuuc c 2158321RNAhomo sapiens 583aucacauugc cagggauuac c
2158422RNAhomo sapiens 584uggcucaguu cagcaggaac ag 2258522RNAhomo
sapiens 585uggcucaguu cagcaggaac ag 2258622RNAhomo sapiens
586cauugcacuu gucucggucu ga 2258722RNAhomo sapiens 587uucaaguaau
ccaggauagg cu 2258822RNAhomo sapiens 588uucaaguaau ccaggauagg cu
2258921RNAhomo sapiens 589uucaaguaau ucaggauagg u 2159021RNAhomo
sapiens 590uucacagugg cuaaguuccg c 2159121RNAhomo sapiens
591uucacagugg cuaaguucug c 2159222RNAhomo sapiens 592cacuagauug
ugagcuccug ga 2259322RNAhomo sapiens 593gaggguuggg uggaggcucu cc
2259422RNAhomo sapiens 594ugguuuaccg ucccacauac au 2259522RNAhomo
sapiens 595uagcaccauc ugaaaucggu ua 2259623RNAhomo sapiens
596uagcaccauu ugaaaucagu guu 2359723RNAhomo sapiens 597uagcaccauu
ugaaaucagu guu 2359822RNAhomo sapiens 598uagcaccauu ugaaaucggu ua
2259923RNAhomo sapiens 599cagugcaaug auauugucaa agc 2360022RNAhomo
sapiens 600uguaaacauc cucgacugga ag 2260122RNAhomo sapiens
601uguaaacauc cuacacucag cu 2260223RNAhomo sapiens 602uguaaacauc
cuacacucuc agc 2360323RNAhomo sapiens 603uguaaacauc cuacacucuc agc
2360422RNAhomo sapiens 604uguaaacauc cccgacugga ag 2260522RNAhomo
sapiens 605uguaaacauc cuugacugga ag 2260621RNAhomo sapiens
606aggcaagaug cuggcauagc u 2160722RNAhomo sapiens 607uauugcacau
uacuaaguug ca 2260822RNAhomo sapiens 608aaaagcuggg uugagagggc ga
2260922RNAhomo sapiens 609aaaagcuggg uugagagggc aa 2261022RNAhomo
sapiens 610aaaagcuggg uugagagggc aa 2261120RNAhomo sapiens
611aaaagcuggg uugagagggu 2061220RNAhomo sapiens 612aaaagcuggg
uugagagggu 2061319RNAhomo sapiens 613aaaagcuggg uugagagga
1961419RNAhomo sapiens 614aaaagcuggg uugagagga 1961521RNAhomo
sapiens 615cacauuacac ggucgaccuc u 2161623RNAhomo sapiens
616cgcauccccu agggcauugg ugu 2361722RNAhomo sapiens 617cuggcccucu
cugcccuucc gu 2261823RNAhomo sapiens 618gcaaagcaca cggccugcag aga
2361921RNAhomo sapiens 619gccccugggc cuauccuaga a 2162023RNAhomo
sapiens 620ucaagagcaa uaacgaaaaa ugu 2362122RNAhomo sapiens
621uccagcauca gugauuuugu ug 2262223RNAhomo sapiens 622ugagcgccuc
gacgacagag ccg 2362321RNAhomo sapiens 623gugcauugua guugcauugc a
2162420RNAhomo sapiens 624gugcauugcu guugcauugc 2062522RNAhomo
sapiens 625uuauaaagca augagacuga uu 2262621RNAhomo sapiens
626aggggugcua ucugugauug a 2162722RNAhomo sapiens 627gcugacuccu
aguccagggc uc 2262822RNAhomo sapiens 628uggcaguguc uuagcugguu gu
2262923RNAhomo sapiens 629uaggcagugu cauuagcuga uug 2363023RNAhomo
sapiens 630aggcagugua guuagcugau ugc 2363122RNAhomo sapiens
631uuaucagaau cuccaggggu ac 2263222RNAhomo sapiens 632aacacaccua
uucaaggauu ca 2263322RNAhomo sapiens 633aauugcacgg uauccaucug ua
2263422RNAhomo sapiens 634uaaugccccu aaaaauccuu au 2263521RNAhomo
sapiens 635aauaauacau gguugaucuu u 2163622RNAhomo sapiens
636gccugcuggg guggaaccug gu 2263720RNAhomo sapiens 637acucaaacug
ugggggcacu 2063822RNAhomo sapiens 638uuauaauaca accugauaag ug
2263922RNAhomo sapiens 639auauaauaca accugcuaag ug 2264022RNAhomo
sapiens 640uuuguucguu cggcucgcgu ga 2264122RNAhomo sapiens
641agagguugcc cuuggugaau uc 2264221RNAhomo sapiens 642acuggacuug
gagucagaag g 2164321RNAhomo sapiens 643ugguagacua uggaacguag g
2164422RNAhomo sapiens 644uauacaaggg caagcucucu gu 2264522RNAhomo
sapiens 645gaaguuguuc gugguggauu cg 2264622RNAhomo sapiens
646agaucagaag gugauugugg cu 2264722RNAhomo sapiens 647gaauguugcu
cggugaaccc cu 2264821RNAhomo sapiens 648aauauaacac agauggccug u
2164921RNAhomo sapiens 649uaguagaccg uauagcguac g 2165023RNAhomo
sapiens 650aucaacagac auuaauuggg cgc 2365123RNAhomo sapiens
651ugaggggcag agagcgagac uuu 2365222RNAhomo sapiens 652cagcagcaau
ucauguuuug aa 2265322RNAhomo sapiens 653uaauacuguc ugguaaaacc gu
2265423RNAhomo sapiens 654ucuuggagua ggucauuggg ugg 2365522RNAhomo
sapiens 655aucaugaugg gcuccucggu gu 2265622RNAhomo sapiens
656uugcauaugu aggauguccc au 2265722RNAhomo sapiens 657uggcagugua
uuguuagcug gu 2265822RNAhomo sapiens 658aggcagugua uuguuagcug gc
2265922RNAhomo sapiens 659uuuugcgaug uguuccuaau au 2266022RNAhomo
sapiens 660uuuugcgaug uguuccuaau au 2266122RNAhomo sapiens
661uuuugcaaua uguuccugaa ua 2266222RNAhomo sapiens 662aacuguuugc
agaggaaacu ga 2266323RNAhomo sapiens 663uagugcaaua uugcuuauag ggu
2366421RNAhomo sapiens 664gcaguccaug ggcauauaca c 2166522RNAhomo
sapiens 665aagacgggag gaaagaaggg ag 2266622RNAhomo sapiens
666ucaggcucag uccccucccg au 2266722RNAhomo sapiens 667agaggcuggc
cgugaugaau uc 2266822RNAhomo sapiens 668uccuguacug agcugccccg ag
2266922RNAhomo sapiens 669aaucguacag ggucauccac uu 2267021RNAhomo
sapiens 670cccagauaau ggcacucuca a 2167122RNAhomo sapiens
671uuguacaugg uaggcuuuca uu 2267222RNAhomo sapiens 672ugaaacauac
acgggaaacc uc 2267322RNAhomo sapiens 673aaacaaacau ggugcacuuc uu
2267421RNAhomo sapiens 674cagcagcaca cugugguuug u 2167521RNAhomo
sapiens 675uuaagacuug cagugauguu u 2167622RNAhomo sapiens
676augcaccugg gcaaggauuc ug 2267722RNAhomo sapiens 677aaugcacccg
ggcaaggauu cu 2267822RNAhomo sapiens 678aaugcaccug ggcaaggauu ca
2267923RNAhomo sapiens 679uagcagcggg aacaguucug cag 2368022RNAhomo
sapiens 680agacccuggu cugcacucua uc 2268122RNAhomo sapiens
681gggagccagg aaguauugau gu 2268223RNAhomo sapiens 682ugauuguagc
cuuuuggagu aga 2368322RNAhomo sapiens 683uacugcagac guggcaauca ug
2268418RNAhomo sapiens 684uucacaggga ggugucau 1868518RNAhomo
sapiens 685uucacaggga ggugucau 1868622RNAhomo sapiens 686uucacaagga
ggugucauuu au 2268722RNAhomo sapiens 687uucucaagga ggugucguuu au
2268822RNAhomo sapiens 688caugccuuga guguaggacc gu 2268922RNAhomo
sapiens 689ugugacagau ugauaacuga aa 2269022RNAhomo sapiens
690aaacauucgc ggugcacuuc uu 2269122RNAhomo sapiens 691caaaacuggc
aauuacuuuu gc 2269222RNAhomo sapiens 692caaaacuggc aauuacuuuu gc
2269322RNAhomo sapiens 693caaaacuggc aauuacuuuu gc 2269422RNAhomo
sapiens 694caagaaccuc aguugcuuuu gu 2269522RNAhomo sapiens
695aaaaacugag acuacuuuug ca 2269622RNAhomo sapiens 696aaaaguaauc
gcgguuuuug uc 2269722RNAhomo sapiens 697aaaaguaauc gcgguuuuug uc
2269822RNAhomo sapiens 698aaaaguaauc gcgguuuuug uc 2269922RNAhomo
sapiens 699aaaaguaauc gcgguuuuug uc 2270022RNAhomo sapiens
700aaaaguaauu gcggucuuug gu 2270122RNAhomo sapiens 701aaaaguacuu
gcggauuuug cu 2270222RNAhomo sapiens 702aaaaguauuu gcggguuuug uc
2270322RNAhomo sapiens 703caaaaguaau uguggauuuu gu 2270423RNAhomo
sapiens 704agugccugag ggaguaagag ccc 2370523RNAhomo sapiens
705agugccugag ggaguaagag ccc 2370621RNAhomo sapiens 706gcgacccaua
cuugguuuca g 2170722RNAhomo sapiens 707cacgcucaug cacacaccca ca
2270822RNAhomo sapiens 708aagaugugga aaaauuggaa uc 2270921RNAhomo
sapiens 709uagauaaaau auugguaccu g 2171022RNAhomo sapiens
710uaacugguug aacaacugaa cc 2271123RNAhomo sapiens 711uuacaguugu
ucaaccaguu acu 2371222RNAhomo sapiens 712uuaugguuug ccugggacug ag
2271322RNAhomo sapiens 713ugagaaccac gucugcucug ag 2271421RNAhomo
sapiens 714uaauuuuaug uauaagcuag u 2171522RNAhomo sapiens
715uacgucaucg uugucaucgu ca 2271622RNAhomo sapiens 716uccgagccug
ggucucccuc uu 2271722RNAhomo sapiens 717agucauugga ggguuugagc ag
2271823RNAhomo sapiens 718aaacucuacu uguccuucug agu 2371921RNAhomo
sapiens 719agggggaaag uucuauaguc c 2172022RNAhomo sapiens
720augcugacau auuuacuaga gg 2272121RNAhomo sapiens 721uggguuuacg
uugggagaac u 2172224RNAhomo sapiens 722aaagacauag gauagaguca ccuc
2472322RNAhomo sapiens 723acuuguaugc uagcucaggu ag 2272422RNAhomo
sapiens 724uuuaggauaa gcuugacuuu ug 2272521RNAhomo sapiens
725aauggcgcca cuaggguugu g 2172622RNAhomo sapiens 726uaugucugcu
gaccaucacc uu 2272722RNAhomo sapiens 727uacccauugc auaucggagu ug
2272821RNAhomo sapiens 728uccgguucuc agggcuccac c 2172923RNAhomo
sapiens 729aaggagcuua caaucuagcu ggg 2373023RNAhomo sapiens
730uggaagacua gugauuuugu ugu 2373123RNAhomo sapiens 731uggaagacua
gugauuuugu ugu 2373223RNAhomo sapiens 732uggaagacua gugauuuugu ugu
2373320RNAhomo sapiens 733cggcucuggg ucugugggga 2073423RNAhomo
sapiens 734ugcaccaugg uugucugagc aug 2373522RNAhomo sapiens
735ugagaccucu ggguucugag cu 2273621RNAhomo sapiens 736gcaggaacuu
gugagucucc u 2173722RNAhomo sapiens 737cugcccuggc ccgagggacc ga
2273820RNAhomo sapiens 738guagaggaga uggcgcaggg 2073922RNAhomo
sapiens 739gugaacgggc gccaucccga gg 2274021RNAhomo sapiens
740uuaauaucgg acaaccauug u 2174122RNAhomo sapiens 741ugcaacgaac
cugagccacu ga 2274221RNAhomo sapiens 742cacugugucc uuucugcgua g
2174323RNAhomo sapiens 743ucuuugguua ucuagcugua uga 2374423RNAhomo
sapiens 744ucuuugguua ucuagcugua uga 2374522RNAhomo sapiens
745uauugcacuu gucccggccu gu 2274622RNAhomo sapiens 746uauugcacuu
gucccggccu gu 2274722RNAhomo sapiens 747uauugcacuc gucccggccu cc
2274823RNAhomo sapiens 748caaagugcug uucgugcagg uag 2374923RNAhomo
sapiens 749ucuuugguua ucuagcugua uga 2375021RNAhomo sapiens
750aaggcagggc ccccgcuccc c 2175122RNAhomo sapiens 751ucuucucugu
uuuggccaug ug 2275222RNAhomo sapiens 752aaauuauugu acaucggaug ag
2275322RNAhomo sapiens 753uucaacgggu auuuauugag ca 2275423RNAhomo
sapiens 754uuuggcacua gcacauuuuu gcu
2375522RNAhomo sapiens 755ugagguagua aguuguauug uu 2275622RNAhomo
sapiens 756aacccguaga uccgaucuug ug 2275722RNAhomo sapiens
757cacccguaga accgaccuug cg 2275821RNAhomo sapiens 758aacauagagg
aaauuccacg u 2175922RNAhomo sapiens 759aucauagagg aaaauccaug uu
2276022RNAhomo sapiens 760uggugggccg cagaacaugu gc 2276121RNAhomo
sapiens 761aucauagagg aaaauccacg u 2176221RNAhomo sapiens
762aucauagagg aaaauccacg u 2176321RNAhomo sapiens 763ccaaaacugc
aguuacuuuu g 2176422RNAhomo sapiens 764gagccuggaa gcuggagccu gc
2276520RNAhomo sapiens 765cgggcguggu ggugggggug 2076621RNAhomo
sapiens 766ucgaggagcu cacagucuag a 2176721RNAhomo sapiens
767uaaggugcau cuagugcagu u 2176817RNAhomo sapiens 768gaggguuggg
uggaggc 1776922RNAhomo sapiens 769aucauagagg aaaauccaug uu
2277021RNAhomo sapiens 770aacauagagg aaauuccacg u 2177120RNAhomo
sapiens 771acuggacuug gagucagaaa 2077220RNAhomo sapiens
772acuggacuug gagucagaaa 2077319RNAhomo sapiens 773acuggacuug
gagucagga 1977422RNAhomo sapiens 774aggcagugua uugcuagcgg cu
2277522RNAhomo sapiens 775ugcacccagg caaggauucu gc 2277622RNAhomo
sapiens 776uaauccuugc uaccugggug ag 2277722RNAhomo sapiens
777aaaaguaauc gcgguuuuug uc 2277822RNAhomo sapiens 778aaaaguaauu
guggauuuug cu 2277922RNAhomo sapiens 779aaaaguaauu guggauuuug cu
2278022RNAhomo sapiens 780ugucuuacuc ccucaggcac au 2278118RNAhomo
sapiens 781ggugggcuuc ccggaggg 1878218RNAhomo sapiens 782gaagcggugg
cugggcug 1878318RNAhomo sapiens 783cacugcagga cucagcag
1878417RNAhomo sapiens 784ugagggagga gacugca 1778520RNAhomo sapiens
785acuggacuug gagccagaag 2078622RNAhomo sapiens 786gucacugaug
ucuguagcug ag 2278722RNAhomo sapiens 787gaugaggaug gauagcaagg aa
2278822RNAhomo sapiens 788accugucugu ggaaaggagc ua 2278921RNAhomo
sapiens 789aaaagcauca ggaaguaccc a 2179022RNAhomo sapiens
790ugccuggaac auaguaggga cu 2279122RNAhomo sapiens 791ugccuggaac
auaguaggga cu 2279220RNAhomo sapiens 792uggauuaaaa acaauggagg
2079321RNAhomo sapiens 793auaggcacca aaaagcaaca a 2179420RNAhomo
sapiens 794acugggcuug gagucagaag 2079522RNAhomo sapiens
795caaaaaccgg caauuacuuu ug 2279622RNAhomo sapiens 796gucaaaugaa
gggcugauca cg 2279727RNAhomo sapiens 797aaagggagga uuugcuuaga
aggaugg 2779822RNAhomo sapiens 798agaguuaacu caaaauggac ua
2279922RNAhomo sapiens 799uguugggauu cagcaggacc au 2280022RNAhomo
sapiens 800uaaauagagu aggcaaagga ca 2280122RNAhomo sapiens
801agagucgaga gugggagaag ag 2280217RNAhomo sapiens 802gaagauggac
guacuuu 1780321RNAhomo sapiens 803ucugaauaga gucugaagag u
2180422RNAhomo sapiens 804caaggagacg ggaacaugga gc 2280522RNAhomo
sapiens 805aagaggaaga aauggcuggu uc 2280621RNAhomo sapiens
806uucgcgggcg aaggcaaagu c 2180720RNAhomo sapiens 807aaaagcuggg
cugagaggcg 2080822RNAhomo sapiens 808auggccaaaa cugcaguuau uu
2280922RNAhomo sapiens 809uaguggauga ugcacucugu gc 2281018RNAhomo
sapiens 810aggcuggagu gagcggag 1881122RNAhomo sapiens 811gaaaacgaca
augacuuuug ca 2281222RNAhomo sapiens 812gcgacucuga aaacuagaag gu
2281321RNAhomo sapiens 813agaaggcugg agcgcggcgg u 2181420RNAhomo
sapiens 814aaagacucug caagaugccu 2081521RNAhomo sapiens
815acaggagugg gggugggaca u 2181618RNAhomo sapiens 816aggagaagua
aaguagaa 1881722RNAhomo sapiens 817auggccagag cucacacaga gg
2281821RNAhomo sapiens 818gcaggacagg cagaagugga u 2181922RNAhomo
sapiens 819aucagggcuu guggaauggg aa 2282022RNAhomo sapiens
820auggccagag cucacacaga gg 2282122RNAhomo sapiens 821ugaggauaug
gcagggaagg gg 2282218RNAhomo sapiens 822aggagguugg uguggauu
1882322RNAhomo sapiens 823uuggaagaca uggagcauga gg 2282422RNAhomo
sapiens 824ucuggcaagu aaaaaacucu ca 2282521RNAhomo sapiens
825ugggcucagg guacaaaggu u 2182621RNAhomo sapiens 826caaaaacugc
aauuacuuuc a 2182721RNAhomo sapiens 827caaaaacugc aauuacuuuc a
2182821RNAhomo sapiens 828gcugcaccgg agacugggua a 2182921RNAhomo
sapiens 829gcugcaccgg agacugggua a 2183022RNAhomo sapiens
830auccccagau acaauggaca au 2283122RNAhomo sapiens 831auuguccuug
cuguuuggag au 2283221RNAhomo sapiens 832cacaggcuua gaaaagacag u
2183322RNAhomo sapiens 833ucgaggacug guggaagggc cu 2283422RNAhomo
sapiens 834gugacugaua ccuuggaggc au 2283522RNAhomo sapiens
835agggcuggac ucagcggcgg ag 2283622RNAhomo sapiens 836ugucgugggg
cuugcuggcu ug 2283717RNAhomo sapiens 837acagggagga gauugua
1783819RNAhomo sapiens 838acuggacuug gaggcagaa 1983917RNAhomo
sapiens 839gccggacaag agggagg 1784022RNAhomo sapiens 840auacacauac
acgcaacaca ca 2284117RNAhomo sapiens 841uuggaggcgu ggguuuu
1784222RNAhomo sapiens 842aauucccuug uagauaaccc gg 2284322RNAhomo
sapiens 843aagacuggag acaaaguggg ag 2284422RNAhomo sapiens
844cugacugaau agguaggguc au 2284518RNAhomo sapiens 845cucgaguugg
aagaggcg 1884622RNAhomo sapiens 846agauuguuuc uuuugccgug ca
2284721RNAhomo sapiens 847cacggcaaaa gaaacaaucc a 2184822RNAhomo
sapiens 848cagggcuggc agugacaugg gu 2284917RNAhomo sapiens
849ggugggggcu guuguuu 1785022RNAhomo sapiens 850uggggaggug
uggagucagc au 2285121RNAhomo sapiens 851gcagagaaca aaggacucag u
2185220RNAhomo sapiens 852ggcuccuugg ucuaggggua 2085322RNAhomo
sapiens 853cgucccgggg cugcgcgagg ca 2285421RNAhomo sapiens
854aaagguaauu gugguuucug c 2185521RNAhomo sapiens 855aaagguaauu
gugguuucug c 2185622RNAhomo sapiens 856uggggauuug gagaaguggu ga
2285720RNAhomo sapiens 857aaaagugauu gcaguguuug 2085817RNAhomo
sapiens 858ugguagagcu gaggaca 1785923RNAhomo sapiens 859uugaauucuu
ggccuuaagu gau 2386022RNAhomo sapiens 860uaggagcuca acagaugccu gu
2286121RNAhomo sapiens 861agcuuuuggg aauucaggua g 2186220RNAhomo
sapiens 862gagcuugguc uguagcgguu 2086320RNAhomo sapiens
863ggauccgagu cacggcacca 2086421RNAhomo sapiens 864caaaagugau
cgugguuuuu g 2186517RNAhomo sapiens 865agggugugug uguuuuu
1786617RNAhomo sapiens 866ccugguggcu uccuuuu 1786722RNAhomo sapiens
867ucacaaggua uugacuggcg ua 2286819RNAhomo sapiens 868agagguaggu
guggaagaa 1986922RNAhomo sapiens 869ccaggaggcg gaggaggugg ag
2287021RNAhomo sapiens 870guggaggacu gagaagguga g 2187121RNAhomo
sapiens 871acugacagga gagcauuuug a 2187222RNAhomo sapiens
872agcgcgggcu gagcgcugcc ag 2287321RNAhomo sapiens 873aagcucgggc
gcuccggcug u 2187422RNAhomo sapiens 874auagugguug ugaauuuacc uu
2287523RNAhomo sapiens 875gauugagacu aguagggcua ggc 2387622RNAhomo
sapiens 876cuaccccagg augccagcau ag 2287721RNAhomo sapiens
877acuggacuug gugucagaug g 2187822RNAhomo sapiens 878gacaaugaug
agaagaccug ag 2287919RNAhomo sapiens 879accgcucgau cuugggacc
1988022RNAhomo sapiens 880uaguggauga uggagacucg gu 2288121RNAhomo
sapiens 881cucgggagcg uuagagaugg a 2188222RNAhomo sapiens
882ggcuggagcg agugcagugg ug 2288322RNAhomo sapiens 883ucaggugugg
aaacugaggc ag 2288423RNAhomo sapiens 884ugacacggag gguggcuugg gaa
2388522RNAhomo sapiens 885gaagauggug cugugcugag ga 2288622RNAhomo
sapiens 886aaagacugca auuacuuuug cg 2288717RNAhomo sapiens
887gagacugggg uggggcc 1788821RNAhomo sapiens 888aagguuugga
uagaugcaau a 2188922RNAhomo sapiens 889aaagguaauu gcaguuuuuc cc
2289022RNAhomo sapiens 890aggggaccaa agagauauau ag 2289121RNAhomo
sapiens 891uaaaaacugc aauuacuuuu a 2189221RNAhomo sapiens
892uaaaaacugc aauuacuuuu a 2189322RNAhomo sapiens 893cucaaguagu
cugaccaggg ga 2289422RNAhomo sapiens 894ucuggcugag gaggaagugg ag
2289522RNAhomo sapiens 895ucuggcugag gaggaagugg ag 2289621RNAhomo
sapiens 896ggcgacaaaa cgagacccug u 2189718RNAhomo sapiens
897gggugcgggc cggcgggg 1889822RNAhomo sapiens 898ccugcugguc
aggaguggau ac 2289922RNAhomo sapiens 899caugcuagga uagaaagaau gg
2290022RNAhomo sapiens 900aauguggaag uggucugagg ca 2290122RNAhomo
sapiens 901gcaaagugau gaguaauacu gg 2290222RNAhomo sapiens
902uggcggcggu aguuaugggc uu 2290318RNAhomo sapiens 903agagcagaag
gaugagau 1890420RNAhomo sapiens 904uggaauggcc ugaaggugga
2090522RNAhomo sapiens 905gcucccucua gggucgcucg ga 2290621RNAhomo
sapiens 906uggcaaacgu ggaagccgag a 2190722RNAhomo sapiens
907uuuguaugga uaugugugug ua 2290822RNAhomo sapiens 908ugaggagauc
gucgagguug gc 2290922RNAhomo sapiens 909ugggaacuua guagagguuu aa
2291018RNAhomo sapiens 910gguggggggu guuguuuu 1891118RNAhomo
sapiens 911gguggggggu guuguuuu 1891222RNAhomo sapiens 912cuagugcucu
ccguuacaag ua 2291322RNAhomo sapiens 913cacuuguaau ggagaacacu aa
2291422RNAhomo sapiens 914uuguggcugg ucaugaggcu aa 2291521RNAhomo
sapiens 915cccugggguu cugaggacau g 2191622RNAhomo sapiens
916caagggacca agcauucauu au 2291722RNAhomo sapiens 917caggaaggau
uuagggacag gc 2291822RNAhomo sapiens 918cuauuaagga cauuugugau uc
2291922RNAhomo sapiens 919auuaaggaca uuugugauug au 2292021RNAhomo
sapiens 920aaaaggcaua aaaccaagac a 2192121RNAhomo sapiens
921aaaaggcaua aaaccaagac a 2192217RNAhomo sapiens 922gaggcugagc
ugaggag 1792322RNAhomo sapiens 923cugggaggug ugauauugug gu
2292420RNAhomo sapiens 924uaaaaacugc aauuacuuuc 2092520RNAhomo
sapiens 925uaaaaacugc aauuacuuuc 2092622RNAhomo sapiens
926ugugauauca ugguuccugg ga 2292722RNAhomo sapiens 927gggaggugug
aucucacacu cg 2292822RNAhomo sapiens 928cugggaggug ugauauugug gu
2292922RNAhomo sapiens 929ugugauauca ugguuccugg ga 2293022RNAhomo
sapiens 930gggaggugug aucucacacu cg 2293122RNAhomo sapiens
931ugugauauca ugguuccugg ga 2293222RNAhomo sapiens 932ugugauaucg
ugcuuccugg ga 2293322RNAhomo sapiens 933cgcgcggccg ugcucggagc ag
2293421RNAhomo sapiens 934ccaggcucug cagugggaac u 2193518RNAhomo
sapiens 935ccaggcucug caguggga 1893621RNAhomo sapiens 936aaaaguaacu
gcgguuuuug a 2193721RNAhomo sapiens 937agccaagugg aaguuacuuu a
2193817RNAhomo sapiens 938ggagugggcu ggugguu 1793918RNAhomo sapiens
939gagcgauccg agggacug 1894022RNAhomo sapiens 940aagggcuucc
ucucugcagg ac 2294122RNAhomo sapiens 941aagggcuucc ucucugcagg ac
2294222RNAhomo sapiens 942aacccagugg gcuauggaaa ug
2294317RNAhomo sapiens 943gggguggucu guuguug 1794420RNAhomo sapiens
944aaaaggcggg agaagcccca 2094522RNAhomo sapiens 945uggcuguugg
agggggcagg cu 2294620RNAhomo sapiens 946uaacggccgc gguacccuaa
2094722RNAhomo sapiens 947uaggauuaca agugucggcc ac 2294817RNAhomo
sapiens 948gcugggcgag gcuggca 1794919RNAhomo sapiens 949agagcuggcu
gaagggcag 1995018RNAhomo sapiens 950agggggcggg cuccggcg
1895119RNAhomo sapiens 951gccgagaguc gucgggguu 1995221RNAhomo
sapiens 952uggggcuagu gaugcaggac g 2195322RNAhomo sapiens
953ugugacuuua agggaaaugg cg 2295422RNAhomo sapiens 954ucucaggagu
aaagacagag uu 2295522RNAhomo sapiens 955agguggaugc aaugugaccu ca
2295622RNAhomo sapiens 956aacggcaaug acuuuuguac ca 2295722RNAhomo
sapiens 957ucugguaaga gauuugggca ua 2295822RNAhomo sapiens
958aauguggacu ggugugacca aa 2295922RNAhomo sapiens 959aauguggacu
ggugugacca aa 2296017RNAhomo sapiens 960ggggcugggc gcgcgcc
1796121RNAhomo sapiens 961agaaggccuu uccaucucug u 2196222RNAhomo
sapiens 962ccagacugug gcugaccaga gg 2296318RNAhomo sapiens
963aggagaagca ggagcugu 1896421RNAhomo sapiens 964aauguaaaca
ggcuuuuugc u 2196522RNAhomo sapiens 965gaggaaacug aagcugagag gg
2296617RNAhomo sapiens 966cuccgggacg gcugggc 1796722RNAhomo sapiens
967ugggcuggca gggcaagugc ug 2296818RNAhomo sapiens 968gaggcugaag
gaagaugg 1896917RNAhomo sapiens 969aagacugaga ggaggga
1797017RNAhomo sapiens 970ugagguagua guuucuu 1797122RNAhomo sapiens
971cggcugggag ccgaggcguc gg 2297221RNAhomo sapiens 972uaugugaccu
cggaugaauc a 2197322RNAhomo sapiens 973gcugaugaug auggugcuga ag
2297421RNAhomo sapiens 974agauguaugg aaucuguaua u 2197522RNAhomo
sapiens 975uuuaagcagg aaauagaauu ua 2297622RNAhomo sapiens
976ugugacaaua gagaugaaca ug 2297718RNAhomo sapiens 977aggcugggcu
gggacgga 1897820RNAhomo sapiens 978aaaugggugg ucugaggcaa
2097920RNAhomo sapiens 979uaggaugggg gugagaggug 2098020RNAhomo
sapiens 980cuggguuggg cugggcuggg 2098122RNAhomo sapiens
981aagggacugg aguggauugg gu 2298222RNAhomo sapiens 982aaggggcugg
aguggauugg gg 2298317RNAhomo sapiens 983gcggggcugg gcgcgcg
1798422RNAhomo sapiens 984acuaaaggau auagaagguu uu 2298522RNAhomo
sapiens 985acuaaaggau auagaagguu uu 2298622RNAhomo sapiens
986acuaaaggau auagaagguu uu 2298722RNAhomo sapiens 987aagcaauacu
guuaccugaa au 2298822RNAhomo sapiens 988ugagggagua ggauguaugg uu
2298922RNAhomo sapiens 989gaagaacugu ugcauuugcc cu 2299022RNAhomo
sapiens 990cagggccuca cuguaucgcc ca 2299122RNAhomo sapiens
991agacugacgg cuggaggccc au 2299218RNAhomo sapiens 992acaggcagga
uuggggaa 1899322RNAhomo sapiens 993aggacuggac ucccggcagc cc
2299422RNAhomo sapiens 994uagugaguua gagaugcaga gc 2299522RNAhomo
sapiens 995acuggccugg gacuaccggg gg 2299617RNAhomo sapiens
996gggagaaggg ucggggc 1799722RNAhomo sapiens 997uggggcggag
cuuccggagg cc 2299822RNAhomo sapiens 998uggggcggag cuuccggagg cc
2299922RNAhomo sapiens 999uggggcggag cuuccggagg cc 22100022RNAhomo
sapiens 1000uggggcggag cuuccggagg cc 22100122RNAhomo sapiens
1001uggggcggag cuuccggagg cc 22100221RNAhomo sapiens 1002cucgugggcu
cuggccacgg c 21100322RNAhomo sapiens 1003agaaggggug aaauuuaaac gu
22100422RNAhomo sapiens 1004agaaggggug aaauuuaaac gu
22100522RNAhomo sapiens 1005agaaggggug aaauuuaaac gu
22100625RNAhomo sapiens 1006aaauaugaug aaacucacag cugag
25100726RNAhomo sapiens 1007gcucagggau gauaacugug cugaga
26100818RNAhomo sapiens 1008cagcagugcg cagggcug 18100922RNAhomo
sapiens 1009uuggacagaa aacacgcagg aa 22101022RNAhomo sapiens
1010gcuaaggaag uccugugcuc ag 22101122RNAhomo sapiens 1011cuggacugag
ccaugcuacu gg 22101221RNAhomo sapiens 1012ugacucugcc uguaggccgg u
21101321RNAhomo sapiens 1013gaccgagagg gccucggcug u 21101422RNAhomo
sapiens 1014uagaggcugg aauagagauu cu 22101522RNAhomo sapiens
1015uagccuucag aucuuggugu uu 22101622RNAhomo sapiens 1016ccacuuggau
cugaaggcug cc 22101722RNAhomo sapiens 1017auagcagcau gaaccugucu ca
22101822RNAhomo sapiens 1018ugagacaggc uuaugcugcu au
22101921RNAhomo sapiens 1019ggggggaugu gcaugcuggu u 21102022RNAhomo
sapiens 1020cagggaggug aaugguucug uc 22102122RNAhomo sapiens
1021gcugacagca gggcuggccg cu 22102222RNAhomo sapiens 1022uggucugcaa
agagaugacu gu 22102322RNAhomo sapiens 1023ucauuauaug uaugaucugg ac
22102421RNAhomo sapiens 1024auuggacugc ugauggcccg u 21102521RNAhomo
sapiens 1025uuggagggug uggaagacau c 21102618RNAhomo sapiens
1026cccagcagga cgggagcg 18102717RNAhomo sapiens 1027auggagaagg
cuucuga 17102817RNAhomo sapiens 1028aaaagcuggg uugagaa
17102922RNAhomo sapiens 1029uggaagguag acggccagag ag
22103022RNAhomo sapiens 1030ucugggaggu uguagcagug ga
22103117RNAhomo sapiens 1031ccccggggag cccggcg 17103222RNAhomo
sapiens 1032uggaaggagg uugccggacg cu 22103322RNAhomo sapiens
1033caagaaccuc aauuaccuuu gc 22103422RNAhomo sapiens 1034aaucugagaa
ggcgcacaag gu 22103522RNAhomo sapiens 1035caccuugcgc uacucagguc ug
22103622RNAhomo sapiens 1036ggaggaaccu uggagcuucg gc
22103717RNAhomo sapiens 1037ggauggagga ggggucu 17103821RNAhomo
sapiens 1038acuggacuag gagucagaag g 21103917RNAhomo sapiens
1039guggaccugg cugggac 17104022RNAhomo sapiens 1040accuggaccc
agcguagaca aa 22104122RNAhomo sapiens 1041caaaaacugc aguuacuuuu gu
22104220RNAhomo sapiens 1042uugggcuggg cuggguuggg 20104321RNAhomo
sapiens 1043ugugguagau auaugcacga u 21104419RNAhomo sapiens
1044agaagggaaa gaacaucaa 19104521RNAhomo sapiens 1045cuguccuaag
guuguugagu u 21104620RNAhomo sapiens 1046aaaaggcauu gugguuuuug
20104722RNAhomo sapiens 1047cgggcggcgg cuguguugcg ca
22104822RNAhomo sapiens 1048uauggaaggg agaagagcuu ua
22104920RNAhomo sapiens 1049cggcggggac ggcgauuggu 20105022RNAhomo
sapiens 1050ccuccugccc uccuugcugu ag 22105122RNAhomo sapiens
1051ugagccgagc ugagcuuagc ug 22105222RNAhomo sapiens 1052gagcuuggau
gagcugggcu ga 22105322RNAhomo sapiens 1053gcugaacugg gcugagcugg gc
22105423RNAhomo sapiens 1054cugggcugaa ugacagugau gag
23105522RNAhomo sapiens 1055gggagccggg gcugugagag ga
22105621RNAhomo sapiens 1056uuaguccugc cuguagguuu a 21105770RNAhomo
sapiens 1057uggugcaaaa guaauugcgg uuuuugccau uaaaaguaau gcggccaaaa
cugcaguuac 60uuuugcaccc 70105863RNAhomo sapiens 1058cugagccugg
aagcuggagc cugcagugag cuaugaucau gucccuguac ucuagccugg 60gca
63105950RNAhomo sapiens 1059acccgggcgu gguggugggg gugggugccu
guaauuccag cuaguuggga 50106068RNAhomo sapiens 1060agucucucuu
cagggcuccc gagacacaga aacagacacc ugcccucgag gagcucacag 60ucuagaca
68106179RNAhomo sapiens 1061ucuuguguua aggugcaucu agugcaguua
gugaagcagc uuagaaucua cugcccuaaa 60ugccccuucu ggcacaggc
79106260RNAhomo sapiens 1062cugccuccac cccgccuggc cugacccagc
cagggcucua ggaggguugg guggaggcaa 60106368RNAhomo sapiens
1063uaaaacgugg auauuccuuc uauguuuacg ugauuccugg uuaaucauag
aggaaaaucc 60auguuuuc 68106468RNAhomo sapiens 1064uaaaaggugg
auauuccuuc uauguuuaug uuauuuaugg uuaaacauag aggaaauucc 60acguuuuc
68106554RNAhomo sapiens 1065acuguuucug uccuuguucu uguuguuauu
acuggacuug gagucagaaa cagg 54106676RNAhomo sapiens 1066aggagagaac
acuggacuug gagucagaaa acuuucaucc aagucauucc cugcucuaag 60ucccauuucu
guucca 76106779RNAhomo sapiens 1067cugacuccag uguccaggcc aggggcagac
aguggacaga gaacagugcc caagaccacu 60ggacuuggag ucaggacau
79106868RNAhomo sapiens 1068ucagguaggc aguguauugc uagcggcugu
uaaugauuuu aacaguugcu aguugcacuc 60cucucugu 68106984RNAhomo sapiens
1069guucccccuc ucuaauccuu gcuaccuggg ugagagugcu uucugaaugc
agugcaccca 60ggcaaggauu cugcaagggg gagu 84107084RNAhomo sapiens
1070guucccccuc ucuaauccuu gcuaccuggg ugagagugcu uucugaaugc
agugcaccca 60ggcaaggauu cugcaagggg gagu 84107160RNAhomo sapiens
1071acaaaaguaa ucgcgguuuu ugucauuacu uuuaacugua aaaaccacgg
uugcuuuugc 60107284RNAhomo sapiens 1072auguuggugc aaaaguaauu
guggauuuug cuauuacuug uauuuauuug uaaugcaaaa 60cccgcaauua guuuugcacc
aacc 84107383RNAhomo sapiens 1073uguuggugca aaaguaauug uggauuuugc
uauuacuugu auuuauuugu aaugcaaaac 60ccgcaauuag uuuugcacca acc
83107474RNAhomo sapiens 1074cuggugcagu gccugaggga guaagagucc
uguuguugua agauaguguc uuacucccuc 60aggcacaucu ccaa 74107573RNAhomo
sapiens 1075gaaaacaacc aggugggcuu cccggagggc ggaacaccca gccccagcau
ccagggcuca 60ccuaccacgu uug 73107654RNAhomo sapiens 1076gaggcggccc
uagcgccauu uugugggagc gaagcggugg cugggcugcg cuug 54107762RNAhomo
sapiens 1077ugguuuuugc ucugagugac cguggugguu gugggaguca cugcaggacu
cagcaggaau 60uc 62107877RNAhomo sapiens 1078ugguggugug ugccuguagu
cuuagcuacu cgggaggcug agggaggaga cugcagugag 60uggaggucac gccacug
77107978RNAhomo sapiens 1079gucagguccu ggacucccau aguuuucagg
cugcuaaaca acagaacgag cacuggacuu 60ggagccagaa gucuuggg
78108077RNAhomo sapiens 1080cucuugguau gaacaucugu guguucaugu
cucucugugc acaggggacg agagucacug 60augucuguag cugagac
77108165RNAhomo sapiens 1081cuggccucug ugccuggaua cuuuauacgu
guaauuguga ugaggaugga uagcaaggaa 60gccgc 65108269RNAhomo sapiens
1082cugggucucc uuucugcuga gaguugaaca cuuguuggga caaccugucu
guggaaagga 60gcuaccuac 69108383RNAhomo sapiens 1083aguucuucug
cagacaaaag caucaggaag uacccaccau guaccagugg gcccuucuug 60augcucuuga
uugcagagga gcc 83108477RNAhomo sapiens 1084uccuuuauug agucccuacu
auguuccagg caccuacgau acccagugcc uggaacauag 60uagggacuca auaaagu
77108576RNAhomo sapiens 1085ccuuuauuga gucccuacua uguuccaggc
accuacgaua cccagugccu ggaacauagu 60agggacucaa uaaagu
76108665RNAhomo sapiens 1086cgccuccaug uuucagcauc uaugucaugg
gcuugguccu ggaguggauu aaaaacaaug 60gaggu 65108780RNAhomo sapiens
1087aucauguacu gcaguugccu uuuuguuccc augcuguuua agccuagcau
aggcaccaaa 60aagcaacaac aguaugugaa 80108841RNAhomo sapiens
1088cacugggcuu ggagucagaa gaccuggcuc cagcccagcu c 41108988RNAhomo
sapiens 1089guauuagguu ggugcaaaag uuauuguggu uuuugcuauu uuuuuuuaau
ggcaaaaacc 60ggcaauuacu uuugcacuaa ccuaguag 88109060RNAhomo sapiens
1090aaagugucaa augaagggcu gaucacgaaa uagcgcauua gcucuuuuuu
ugaaaacuug 60109170RNAhomo sapiens 1091gacuggcuac guaguucggg
caaauccucc aaaagggaaa gggaggauuu gcuuagaagg 60auggcgcucc
70109286RNAhomo sapiens 1092cuuacaucac acacagaguu aacucaaaau
ggacuaauuu uuccacuagu uaguccauuu 60caaguuaacu cuguguguga uguagu
86109384RNAhomo sapiens 1093gugcuuuaca ugaauggucc cauugaaucc
caacagcuuu gcgaaguguu guugggauuc 60agcaggacca uucguguaaa guaa
84109479RNAhomo sapiens 1094uaaaugguua uguccuuugc cuauucuauu
uaagacaccc uguaccuuaa auagaguagg 60caaaggacag aaacauuuu
79109576RNAhomo sapiens 1095gcagagucga gagugggaga agagcggagc
gugugagcag uacugcggcc uccucuccuc 60uccuaaccuc gcucuc
76109662RNAhomo sapiens 1096aguugaagau ggacguacuu ugucugacua
caauauucaa aaggagucua cucuucaucu 60ug 62109768RNAhomo sapiens
1097gaagccucuu ggggcuuauu uagacaaugg uuucaucauu ucgucugaau
agagucugaa 60gagucuuu 68109873RNAhomo sapiens 1098uuggcaggug
ccauguugcc ugcuccuuac uguacacgug gcuggcaagg agacgggaac 60auggagccgc
cau 73109973RNAhomo sapiens 1099aggaagaaga ggaagaaaug gcugguucuc
aggugaaugu gucuggguuc aggggaugug 60ucuccucuuu ucu 73110069RNAhomo
sapiens 1100ggcgggcuuc gcgggcgaag gcaaagucga uuuccaaaag ugacuuuccu
cacucccgug 60aagucggcg 69110173RNAhomo sapiens 1101agggagaaaa
gcugggcuga gaggcgacug gugucuaauu uguuugucuc uccaacucag 60acugccuggc
cca
73110276RNAhomo sapiens 1102cugcaaaaau aauugcaguu uuugccauua
uuuuuaauaa uuauaauaau ggccaaaacu 60gcaguuauuu uugcac
76110376RNAhomo sapiens 1103cuacuuccag uaguggauga ugcacucugu
gcagggccaa cugugcacac agugcuucau 60ccacuacugg aagugu
76110449RNAhomo sapiens 1104gugaggcugg agugagcgga gaucguacca
cugcacucca accugguga 49110582RNAhomo sapiens 1105cuguuagguu
ggugcaaaag uaauuguggu uuuugaaagu aacuuggcga aaacgacaau 60gacuuuugca
ccaaucuaau ac 82110694RNAhomo sapiens 1106ugguuugcga cucugaaaac
uagaagguuu augacugggc auuucucacc caaugcccaa 60uauugaacuu ucuaguuguc
agagucauua accc 94110770RNAhomo sapiens 1107gcacugcggu ucugaggccg
uuacuccggc uucuccauag agggcggaga aggcuggagc 60gcggcgguga
70110884RNAhomo sapiens 1108gcaucuugca gagccguucc aaugcgacac
cucuagagug ucauccccua gaaugucacc 60uuggaaagac ucugcaagau gccu
84110981RNAhomo sapiens 1109cauccuccuu acgucccacc ccccacuccu
guuucuggug aaauauucaa acaggagugg 60gggugggaca uaaggaggau a
81111053RNAhomo sapiens 1110ucacuuuagg agaaguaaag uagaacuuug
guuuucaacu uuuccuacag ugu 53111180RNAhomo sapiens 1111aggcagcaaa
uggccagagc ucacacagag ggaugagugc acuucaccug cagugugacu 60cagcaggcca
acagaugcua 80111285RNAhomo sapiens 1112gccucacuuu uccacuuaug
ccugcccugc cccucgaauc ugcuccacga uuugggcagg 60acaggcagaa guggauaagu
gagga 85111366RNAhomo sapiens 1113ggcccaucag ggcuugugga augggaagga
gaagggacgc uuccccuucu gcaggccugc 60ugggug 66111474RNAhomo sapiens
1114gcaaauggcc agagcucaca cagagggaug agugcacuuc accugcagug
ugacucagca 60ggccaacaga ugcu 74111570RNAhomo sapiens 1115acguggugag
gauauggcag ggaaggggag uuucccucua uucccuuccc cccaguaauc 60uucaucaugc
70111660RNAhomo sapiens 1116uaugaggagg uuggugugga uucuguugaa
gaaaaagaag gggaacacua auuuuccauu 60111772RNAhomo sapiens
1117uuuuggaaga cauggagcau gagguaagug ccuagauccu caaaccacuu
gccuccaccu 60augcuuccag gu 72111868RNAhomo sapiens 1118cuuccucugg
caaguaaaaa acucucauuu uccuuaaaaa augagaguuu uuuacuugca 60auaggaaa
68111960RNAhomo sapiens 1119acuuugugca uuggguccac aaggagggga
ugacccuugu gggcucaggg uacaaagguu 60112070RNAhomo sapiens
1120gcaguuuuug ccauuaaguu gcgguuuuug ccauuauaau ggcaaaaacu
gcaauuacuu 60ucacaccugc 70112167RNAhomo sapiens 1121ugugcaaaag
uaauuguggu uuuugucauu uaaaaguaau ggcaaaaacu gcaauuacuu 60ucacacc
67112277RNAhomo sapiens 1122acuugucaug ucuuacccag ucuccggugc
agccuguugu caaggcugca ccggagacug 60gguaagacau gacaagc
77112370RNAhomo sapiens 1123ugucuuaccc agucuccggu gcagccuuga
caacaggcug caccggagac uggguaagac 60augacaaguu 70112477RNAhomo
sapiens 1124cgugucaucc ccagauacaa uggacaauau gcuauuauaa ucguauggca
uuguccuugc 60uguuuggaga uaauacu 77112577RNAhomo sapiens
1125cgugucaucc ccagauacaa uggacaauau gcuauuauaa ucguauggca
uuguccuugc 60uguuuggaga uaauacu 77112693RNAhomo sapiens
1126uaaguguaaa cuuaaggacu gucuuuucua agccugugcc uugccuuucc
uuuggcacag 60gcuuagaaaa gacagucuuu aaguuuacac uuc 93112773RNAhomo
sapiens 1127ucucagaguc gaggacuggu ggaagggccu uuccccucag accaaggccc
uggccccagc 60uucuucucag agu 73112880RNAhomo sapiens 1128ccagugacug
auaccuugga ggcauuuuau cuaagauaca cacaaagcaa augccucuaa 60gguaucaguu
uaccaggcca 80112971RNAhomo sapiens 1129gcgcagaggg cuggacucag
cggcggagcu ggcugcuggc cucaguucug ccucugucca 60gguccuugug a
71113098RNAhomo sapiens 1130cucucaccaa gcaagugcag uggggcuugc
uggcuugcac cgugacuccc ucucaccaag 60caagugucgu ggggcuugcu ggcuugcacu
gugaagau 981131100RNAhomo sapiens 1131cagagucucc uucguguaca
gggaggagac uguacgugag agauagucag auccgcaugu 60uagagcagag ucuccuucgu
guacagggag gagauuguac 100113259RNAhomo sapiens 1132uggucauuga
gucuucaagg cuaguggaaa gagcacugga cuuggaggca gaaagaccc
59113367RNAhomo sapiens 1133gcgcccuccc ucucuccccg gugugcaaau
gugugugugc gguguuaugc cggacaagag 60ggaggug 67113488RNAhomo sapiens
1134augugugugu auaugugugu ugcaugugug uauaugugug uauauaugua
cacauacaca 60uacacgcaac acacauauau acaugcac 88113553RNAhomo sapiens
1135gguggggguu ggaggcgugg guuuuagaac cuaucccuuu cuagcccuga gca
53113672RNAhomo sapiens 1136cgaucacuag auuaucuaca agggaauuuu
uuuuuaauuu aaaaaauucc cuuguagaua 60acccgguggu ca 72113777RNAhomo
sapiens 1137caccaccaaa aucuccaggg gcaucguuga aaucguaagg gaugugcagc
ucauuaagac 60uggagacaaa gugggag 77113866RNAhomo sapiens
1138aaacugacug aauagguagg gucauuuuuc ugugacugca cauggcccaa
ccuauucagu 60uaguuc 66113974RNAhomo sapiens 1139gugacgacug
gccccgccuc uuccucucgg ucccauauug aacucgaguu ggaagaggcg 60aguccggucu
caaa 74114070RNAhomo sapiens 1140uuccugcaga uuguuucuuu ugccgugcaa
guuuaaguuu uugcacggca aaagaaacaa 60uccagagggu 70114170RNAhomo
sapiens 1141uuccugcaga uuguuucuuu ugccgugcaa guuuaaguuu uugcacggca
aaagaaacaa 60uccagagggu 70114267RNAhomo sapiens 1142cugguccauu
ucccugccau ucccuuggcu ucaauuuacu cccagggcug gcagugacau 60gggucaa
67114391RNAhomo sapiens 1143guucuagagc augguuucuc aucauuugca
cuacugauac uuggggucag auaauuguuu 60gugguggggg cuguuguuug cauuguagga
u 91114469RNAhomo sapiens 1144gggcaugggg agguguggag ucagcauggg
gcuaggaggc cccgcgcuga cccgccuucu 60ccgcagcug 69114591RNAhomo
sapiens 1145accugagcac cauuuacuga guccuuuguu cucuacuagu uuguaguagu
ucguagcaga 60gaacaaagga cucaguaaau ggugcucagg a 91114686RNAhomo
sapiens 1146aggagugacc aaaagacaag agugcgagcc uucuauuaug cccagacagg
gccaccagag 60ggcuccuugg ucuaggggua augcca 86114766RNAhomo sapiens
1147agcagcccuc ggcggcccgg ggggcgggcg gcggugcccg ucccggggcu
gcgcgaggca 60caggcg 66114866RNAhomo sapiens 1148gugcaaaggu
aauugugguu ucugcuuuua aagguaaugg caaauauuac auuuacuuuu 60gcacca
66114964RNAhomo sapiens 1149ugcaaaggua auugugguuu cugccauuga
aaguaaaggc aagaaccuca auuaccuuug 60cagc 64115065RNAhomo sapiens
1150ugucugggga uuuggagaag uggugagcgc aggucuuugg caccaucucc
ccuggucccu 60uggcu 65115176RNAhomo sapiens 1151agguuggugc
aaaagugauu gcaguguuug ccaauaaaag uaaugacaaa aacugcaguu 60acuuuugcac
cagccc 76115266RNAhomo sapiens 1152ucuguaccuc agcuuugcuc ccaaccaacc
acuuccacau guuuugcugg uagagcugag 60gacagc 66115371RNAhomo sapiens
1153uggaucacuu gaggccaaga gugcaaggcu guagugugca cagccuugaa
uucuuggccu 60uaagugaucc c 71115470RNAhomo sapiens 1154ucagaguagg
agcucaacag augccuguug acugaauaau aaacagguau cgcaggagcu 60uuuguuaugu
70115596RNAhomo sapiens 1155uguccucuug agguaccuga auuaccaaaa
gcuuuaugua uucugaaguu auugaaaaua 60agagcuuuug ggaauucagg uaguucagga
gugacu 96115688RNAhomo sapiens 1156uggagagcuu ggucuguagc gguuuccuug
gggcaggugg ggacugcucc uuugggagga 60aggaggaggc ccaggccgcg ucuucagg
88115755RNAhomo sapiens 1157ccggauccga gucacggcac caaauuucau
gcguguccgu gugaagagac cacca 55115868RNAhomo sapiens 1158guggugcaaa
agugaucgug guuuuugcaa uuuuuuaaug acaaaaacca caauuacuuu 60ugcaccaa
68115958RNAhomo sapiens 1159agaagggugu guguguuuuu ccugagaaua
agagaaggaa ggacagccaa auucuuca 58116043RNAhomo sapiens
1160augaaccugg uggcuuccuu uucugggagg aaguuagggu uca 43116168RNAhomo
sapiens 1161ggaguacucc agucaauacc gugugaguua gaaaagcuca auucacaagg
uauugacugg 60cguauuca 68116275RNAhomo sapiens 1162gagcgcacag
agguaggugu ggaagaaagu gaaacacuau uuuagguuuu aguuacacuc 60ugcuguggug
ugcug 75116366RNAhomo sapiens 1163acccaggagg cggaggaggu ggagguugca
gugagccaag aucguggcac ugacuccagc 60cugggg 66116475RNAhomo sapiens
1164augaugugga ggacugagaa ggugaggcag uuuugccccg ugcugccuuc
caccgguuaa 60gaccuccaaa aucga 75116576RNAhomo sapiens
1165ggacaaaauu aaaaugcucu ucugucauug uaauaguuca uaugggcacu
gacaggagag 60cauuuugacu uuguca 76116657RNAhomo sapiens
1166gguucacugg ucgugcuucc ugcgggcuga gcgcgggcug agcgcugcca gucagcg
57116766RNAhomo sapiens 1167gcucagucag cugggccgcc ucagcucucg
gaguaggaag cucgggcgcu ccggcuguaa 60ggagcc 66116886RNAhomo sapiens
1168guuuuuugcc cauagugguu gugaauuuac cuucuccucu uugcagugau
aaaggaggua 60aauucacaac cacugugggc agaaac 86116974RNAhomo sapiens
1169gaguaggcuu agguuaugua cguagucuag gccauacgug uuggagauug
agacuaguag 60ggcuaggccu acug 74117074RNAhomo sapiens 1170auagcugguu
ggcauucugg cccugguuca ugccaacucu uguguugacu accccaggau 60gccagcauag
uugc 74117183RNAhomo sapiens 1171acaggaacac uggacuuggu gucagauggg
augagcccug gcucuguuuc cuagcagcaa 60ucugaucuug agcuagucac ugg
83117280RNAhomo sapiens 1172ggggacaaug augagaagac cugaggauuu
gcagccccca gcccuggguu caagucccag 60cucuaccccu ucuuggcccc
80117380RNAhomo sapiens 1173guuucaccgc ucgaucuugg gacccaccgc
ugcccucagc uccgagucca gggcgaggua 60agggcuggag ucgggcagga
80117492RNAhomo sapiens 1174auugaggcac uggguagugg augauggaga
cucgguaccc acugcugagg guggggacca 60agucugcguc auccucuccu cagugccuca
aa 92117577RNAhomo sapiens 1175gucucgggag cguuagagau ggagacuaac
gucuuccaag ggagauugcg ucuccacuuu 60cacccuggua cugagag
77117668RNAhomo sapiens 1176ugcccaggcu ggagcgagug caguggugca
gucaguccua gcucacugca gccucgaacu 60ccugggcu 68117767RNAhomo sapiens
1177uuuucaggug uggaaacuga ggcaggaggc agugaaguaa cuugcucagg
uugcacagcu 60gggaagu 67117858RNAhomo sapiens 1178cuucccagcu
gcccuaaguc aggaguggcu uuccugacac ggaggguggc uugggaaa
58117966RNAhomo sapiens 1179ggugaagaug gugcugugcu gaggaaaggg
gaugcagagc ccugcccagc accaccaccu 60ccuaug 66118068RNAhomo sapiens
1180auggugcaaa aguaaugugg uuuuuuucuu uacuuuuaau ggcaaagacu
gcaauuacuu 60uugcgcca 68118167RNAhomo sapiens 1181aauagauuau
uggucaccac cuccaguuuc ugaauuugug agacuggggu ggggccugag 60aauuugc
67118292RNAhomo sapiens 1182ggaaccuuag uaagguuugg auagaugcaa
uaaaguaugu ccacagcuga aaggacauac 60uuuauugcau guauccaaac cuuacuaauu
ca 92118388RNAhomo sapiens 1183guauuagguu ggugcaaagg uaauugcagu
uuuucccauu uaaaauaugg aaaaaaaaau 60cacaauuacu uuugcaucaa ccuaauaa
88118483RNAhomo sapiens 1184gaaacuacac uuuaagggga ccaaagagau
auauagauau cagcuaccua uauaccuguu 60cggucucuuu aaaguguagu uua
83118572RNAhomo sapiens 1185auugguguaa aaguaauugc agguuaugcc
auuaaaagua augguaaaaa cugcaauuac 60uuuuacacua ac 72118692RNAhomo
sapiens 1186aagguauuag guuggugcaa aaguaauugc aguuuuugcu auuacuuuua
augguaaaaa 60cugcaauuac uuuuacacca accuaauauu ua 92118770RNAhomo
sapiens 1187cauguguccc cuggcacgcu auuugagguu uacuauggaa ccucaaguag
ucugaccagg 60ggacacauga 70118855RNAhomo sapiens 1188gcucuaguag
ccacagccau ccccuagagg gaucuggcug aggaggaagu ggagg 55118971RNAhomo
sapiens 1189ccgccucagu ggcuuccucc acagccaccu ccggagggau cuggcugagg
aggaagugga 60ggugucacug g 71119065RNAhomo sapiens 1190cugggcgaca
aaacgagacc cugucuuuuu uuuuuucuga gacagagucu cguucuguug 60cccaa
65119154RNAhomo sapiens 1191acgcgggugc gggccggcgg gguagaagcc
acccggcccg gcccggcccg gcga 54119271RNAhomo sapiens 1192gccauuccug
cuggucagga guggauacug gagcaauaga uacaguucca cacugacacu 60gcagaagugg
a 71119398RNAhomo sapiens 1193auuuucuuug cuaagucccu ucuuucuauc
cuaguauaac uugaagaauu caaauaguca 60ugcuaggaua gaaagaaugg gacuuggcca
gggaagaa 98119462RNAhomo sapiens 1194gaauagaaag aauguggaag
uggucugagg cauauagagu auaugccaag aacacuacca 60ua 62119572RNAhomo
sapiens 1195acaguaacuu uuauucucau uuuccuuuuc ucuaccuugu agagaagcaa
agugaugagu 60aauacuggcu gg 72119663RNAhomo sapiens 1196ugguggcggc
gguaguuaug ggcuucucuu ucucaccagc agccccuggg ccgccgccuc 60ccu
63119764RNAhomo sapiens 1197agucuucucc uggggcuuug guggcuaugg
uugacugggc cacucagagc agaaggauga 60gaug 64119867RNAhomo sapiens
1198ccuggcagcc cucuggccua guucccacca cacaugaggu gguggaaugg
ccugaaggug 60gaacaga 67119979RNAhomo sapiens 1199ccgacgcgga
gagcggcucu agguggguuu ggcggcggcg aggacaccgc cgcucccucu 60agggucgcuc
ggagcguga 79120072RNAhomo sapiens 1200cgagccucuu ucggcuuucc
aguuugucuc gguccuuugg aacguggcaa acguggaagc 60cgagagggcu cu
72120187RNAhomo sapiens 1201auauacauac auguacacac acaugucauc
cacacacaua cauauauaua uguuuguaug 60gauaugugug uguaugugug uguauac
87120276RNAhomo sapiens 1202aaagcaggcc aaccucgagg aucuccccag
ccuuggcguu caggugcuga ggagaucguc 60gagguuggcc ugcuuc
76120383RNAhomo sapiens 1203ccaaauuuaa aacuuaaacc ucuacuaagu
uuccaugaaa agaacccaug ggaacuuagu 60agagguuuaa guuuuaaauu uga
83120480RNAhomo sapiens 1204uggcagaccc uugcucucuc acucucccua
auggggcuga agacagcuca ggggcagggu 60gggggguguu guuuuuguuu
80120567RNAhomo sapiens 1205uggugggggu gggggguguu guuuuuguuu
uugagacaga gucuugcucc gucgcccagg 60ccggagu 67120691RNAhomo sapiens
1206aaggaacagg ggacacuugu aauggagaac acuaagcuau ggacugcuau
ggacugcuag 60ugcucuccgu uacaaguauc cccuguuacc u 91120794RNAhomo
sapiens 1207aaaggaacag gggacacuug uaauggagaa cacuaagcua uggacugcua
uggacugcua 60gugcucuccg uuacaaguau ccccuguuac cuug 94120878RNAhomo
sapiens 1208uugccuaccu uguuagucuc augaucagac acaaauaugg cucuuugugg
cuggucauga 60ggcuaacaag guaggcac 78120970RNAhomo sapiens
1209augcugcccu gggguucuga ggacaugcuc ugacuccccu gauguccucu
guuccucagg 60ugcugggcga 70121061RNAhomo sapiens 1210aucucaauga
gugugugguu cuaaaugacu cauagucaag ggaccaagca uucauuauga 60a
61121170RNAhomo sapiens 1211aaaagccugu cccuaagucc cucccagccu
uccagaguug gugccaggaa ggauuuaggg 60acaggcuuug 70121281RNAhomo
sapiens 1212uccuccuccc aucaaucaca aauguccuua auggcauuua aggauugcua
uuaaggacau 60uugugauuca cgggaggagg u 81121381RNAhomo sapiens
1213accuccuccc gugaaucaca aauguccuua auagcaaucc uuaaaugcca
uuaaggacau 60uugugauuga ugggaggagg a 81121484RNAhomo sapiens
1214cuuuuuuugu ugcuugucuu gguuuuaugc cuuuuaugug
ccuugauaua aaaggcauaa 60aaccaagaca agcaacagaa aaac 84121584RNAhomo
sapiens 1215cugucaguuu uucuguugcu ugucuugguu uuaugccuuu uauaucaagg
cacauaaaag 60gcauaaaacc aagacaagca acaa 84121686RNAhomo sapiens
1216ggccgaggcu gagcugagga gccuccaaac cuguagacag ggucaugcag
uacuaggggc 60gagccucauc cccugcagcc cuggcc 86121772RNAhomo sapiens
1217gggaggugug auaucguggu uccugggagg ugugauaucg ugguuccugg
gaggugugau 60auugugguuc cu 721218100RNAhomo sapiens 1218augccaaaua
uuagguuggc acaaaaguaa uuguggcuuu ugccauuaaa aguaauggua 60aaaacugcaa
uuacuuucgu gccaaccuaa uauuugugug 100121965RNAhomo sapiens
1219agugcaaaag uaauugcagu uuuugcguua cuuucaaucg uaaaaacugc
aauuacuuuc 60acacc 65122085RNAhomo sapiens 1220gcucccuggg
aggugugaua ucaugguucc ugggaggugu gauccugugc uuccugggag 60gugugauauc
gugguuccug ggagg 85122174RNAhomo sapiens 1221ugggaggugu gaucucacac
ucgcugggag gugugcuauc gucuuccccg ggagguguga 60uccuguucuu ccug
74122272RNAhomo sapiens 1222gggaggugug auaucauggu uccugggagg
ugugaucccg ugcuuccugg gaggugugau 60auugugguuc cu 72122372RNAhomo
sapiens 1223gggaggugug auaucauggu uccugggagg ugugaucccg ugcuuccugg
gaggugugau 60auugugguuc cu 72122480RNAhomo sapiens 1224acugggaggu
gugaucucac acucgcuggg aggugugcua ucgucuuccc ugggaggugu 60gauccuguuc
uuccugagcg 80122572RNAhomo sapiens 1225gggaggugug auaucauggu
uccugggagg uaugauaucg ugguuccugg gaggugugau 60cccgugcucc cu
72122666RNAhomo sapiens 1226aggugugaua ucgugcuucc ugggacgugu
gaugcugugc uuccugggag gugugauccc 60acacuc 66122771RNAhomo sapiens
1227gaaaccaagu ccgagcgugg cuggcgcggg aaaguucggg aacgcgcgcg
gccgugcucg 60gagcagcgcc a 71122892RNAhomo sapiens 1228ccuguuccgg
gcaucaccuc ccacugcaga gccuggggag ccggacagcu cccuucccag 60gcucugcagu
gggaacugau gccuggaaca gu 92122956RNAhomo sapiens 1229ccacugcaga
gccugggaag ggagcugucc ggcuccccag gcucugcagu gggagu 56123057RNAhomo
sapiens 1230gugcaaaagu aacugcgguu uuugagaagu aauugaaaac cgcaauuacu
uuugcag 57123171RNAhomo sapiens 1231gcagagguga guugaccucc
acagggccac ccagggagua aguagccaag uggaaguuac 60uuuaccucug u
71123260RNAhomo sapiens 1232ggagugggcu ggugguuuuu uaagaggaag
ggagaccuaa gcuagcacau gagcacgcuc 60123356RNAhomo sapiens
1233cggcuucccg cgguccccgg ugcugaggag agagcgaucc gagggacugc gccgcc
56123491RNAhomo sapiens 1234acauuauuca ggccgguccu gcagagagga
agcccuucca auaccuguaa gcagaagggc 60uuccucucug caggaccggc cugaauaaug
a 91123599RNAhomo sapiens 1235ggaucauuau ucaggccggu ccugcagaga
ggaagcccuu cugcuuacag guauuggaag 60ggcuuccucu cugcaggacc ggccugaaua
auguaauca 99123670RNAhomo sapiens 1236agugagcaac ccagugggcu
auggaaaugu guggaagaug gcauuucuau uucucagugg 60ggcucuuacc
70123762RNAhomo sapiens 1237aaaaaacaac auacuuagug cauacccaua
uaauauuagg gguggucugu uguuguuuuu 60cu 62123883RNAhomo sapiens
1238ggguuuccuc ugccuuuuuu uccaaugaaa auaacgaaac cuguuauuuc
ccauugaggg 60ggaaaaaggc gggagaagcc cca 83123969RNAhomo sapiens
1239ggagccagcc cuccucccgc acccaaacuu ggagcacuug accuuuggcu
guuggagggg 60gcaggcucg 69124057RNAhomo sapiens 1240agaggcaccg
ccugcccagu gacaugcguu uaacggccgc gguacccuaa cugugca 57124160RNAhomo
sapiens 1241ccuaggauua caagugucgg ccacgggcug ggcacagugg cucacgccug
uaaucccagc 60124263RNAhomo sapiens 1242gcaugcuggg cgaggcuggc
aucuagcaca ggcgguagau gcuugcucuu gccauugcaa 60uga 63124373RNAhomo
sapiens 1243acuguccuuc agccagagcu ggcugaaggg cagaagggaa cuguccuuca
gccagagcug 60gcugaagggc aga 73124462RNAhomo sapiens 1244gguagggggc
gggcuccggc gcugggaccc cacuagggug gcgccuuggc cccgccccgc 60cc
62124556RNAhomo sapiens 1245ucgccgagag ucgucggggu uuccugcuuc
aacagugcuu ggacggaacc cggcgc 56124662RNAhomo sapiens 1246gggggugggg
cuagugaugc aggacgcugg ggacuggaga aguccugccu gacccugucc 60ca
62124778RNAhomo sapiens 1247uggaaacugu gacuuuaagg gaaauggcgc
acagcagacc cugcaaucau gccguuuugc 60uugaagucgc aguuuccc
78124890RNAhomo sapiens 1248aacuugaagg uagggaacuc ugucuucacu
caugaguacc uuccaacacg agcucucagg 60aguaaagaca gaguucccua ccuucaaugu
90124961RNAhomo sapiens 1249caagguggau gcaaugugac cucaacucuu
gguccucuga ggucacauug uauccaccuu 60a 61125097RNAhomo sapiens
1250ggucggugca aaaguaauug cuguuuuugc cauuaaaaau aauggcauua
aaaguaaugg 60caaaaacggc aaugacuuuu guaccaaucu aauaucu
97125184RNAhomo sapiens 1251auaguuucug caaugcucaa aucucuggcc
aaagaccaga acuuaauggu cucugguaag 60agauuugggc auauuagaaa cuaa
84125268RNAhomo sapiens 1252acauuugguc acaccagucc acauuaacgu
ggaccagaca auauuaaugu ggacuggugu 60gaccaaaa 68125382RNAhomo sapiens
1253accuggacau uuggucacac caguccacau uaacguggac cagacaauau
uaauguggac 60uggugugacc aaaaguccag gc 82125480RNAhomo sapiens
1254cugcagcgug cuucuccagg ccccgcgcgc ggacagacac acggacaagu
cccgccaggg 60gcugggcgcg cgccagccgg 80125573RNAhomo sapiens
1255ccagagaugg gaaggccuuc cggugauuau cacagccaug ccuuuaccuc
cagaaggccu 60uuccaucucu guc 73125684RNAhomo sapiens 1256aguuuuaguu
acccugguca ucugcagucu gaaaauacaa aauggaaaau uccagacugu 60ggcugaccag
agguaacuga aacc 84125742RNAhomo sapiens 1257cugaggagaa gcaggagcug
ucuugguaca uucaggucac ug 42125866RNAhomo sapiens 1258aagaaaugua
aacaggcuuu uugcucagug gaguuauuuu gagcaaaaag cuuauuuaca 60uuucug
66125961RNAhomo sapiens 1259acaucagcuc auauaauccu cgaagcugcc
uuuagaaaug aggaaacuga agcugagagg 60g 61126089RNAhomo sapiens
1260accuccggga cggcugggcg ccggcggccg ggagauccgc gcuuccugaa
ucccggccgg 60cccgcccggc gcccguccgc ccgcggguc 89126166RNAhomo
sapiens 1261agggcugggc uggcagggca agugcugcag aucuuugucu aagcagcccc
ugccuuggau 60cuccca 66126268RNAhomo sapiens 1262cucaggcuca
guggugcaug cuuauagucc cagccacucu ggaggcugaa ggaagauggc 60uugagccu
68126369RNAhomo sapiens 1263aagacugaga ggagggaacu ggugaguugu
acauagaaau gcuuucuaac uccuugucuc 60agucuguuu 69126476RNAhomo
sapiens 1264caggagagaa aguacugccc agaagcuaaa guguagauca aacgcauaau
ggcugaggua 60guaguuucuu gaacuu 76126570RNAhomo sapiens
1265ggcggcuggg agccgaggcg ucggugcaga ccuggagacg ggcauggggg
ggcugcggcu 60gcuggcugug 70126664RNAhomo sapiens 1266uaugugaccu
cggaugaauc acugaaauau gucugagcuu cuguuucauc agaugucaca 60uuuu
64126781RNAhomo sapiens 1267agccuuuagc aaguuguaau cuuuuugcug
auggaggguc uugccuccau ggggauggcu 60gaugaugaug gugcugaagg c
81126860RNAhomo sapiens 1268gagauguaug gaaucuguau auaucuauau
auauguguau auauagauuc cauaaaucua 60126983RNAhomo sapiens
1269acaauguaga uauuuaagca ggaaauagaa uuuacauaua aauuucuauu
uguuucuauu 60uccugcuuaa auaucuacau ugc 83127092RNAhomo sapiens
1270cuaagauaau guccuccagg uucaucucug uugucauuug uggcauggac
cauuugugac 60aauagagaug aacauggagg auauuaucuu aa 92127173RNAhomo
sapiens 1271ggaggcuggg cugggacgga cacccggccu ccacuuucug uggcagguac
cuccuccaug 60ucggcccgcc uug 73127277RNAhomo sapiens 1272uggccucugc
caucagacca ucuggguuca aguuuggcuc caucuuuaug aaaugggugg 60ucugaggcaa
guggucu 77127384RNAhomo sapiens 1273auggucccuc ccaauccagc
cauuccucag accagguggc ucccgagcca ccccaggcug 60uaggaugggg gugagaggug
cuag 84127452RNAhomo sapiens 1274ucugggcuga gccgagcugg guuaagccga
gcuggguugg gcugggcugg gu 52127580RNAhomo sapiens 1275ggaagggacu
ggaguggauu ggguacaucu auuauagugg gagcaccuac uacaacccgu 60cccucaagag
ucgagucacc 80127661RNAhomo sapiens 1276cuggugaauc uggguccgcc
agcccccagg gaaggggcug gaguggauug gggaaaucca 60u 61127770RNAhomo
sapiens 1277aggacccagc ggggcugggc gcgcggagca gcgcugggug cagcgccugc
gccggcagcu 60gcaagggccg 70127894RNAhomo sapiens 1278cuuuaauacu
aucucaaacu aaaggauaua gaagguuuuc ccuuucucuu gcccugaaac 60cuucuguauc
cuuuauuuug agauaguauu agaa 94127994RNAhomo sapiens 1279cuuuaauacu
aucucaaacu aaaggauaua gaagguuuuc ccuuucucuu gcccugaaac 60cuucuguauc
cuuuauuuug agauaguauu agaa 94128094RNAhomo sapiens 1280cuuuaauacu
aucucaaacu aaaggauaua gaagguuuuc ccuuucucuu gcccugaaac 60cuucuguauc
cuuuauuuug agauaguauu agaa 94128177RNAhomo sapiens 1281cucaaagaag
caauacuguu accugaaaua ggcugcgaag auaacaguau uucagauaac 60aguauuacau
cuuugaa 77128268RNAhomo sapiens 1282guguauguga gggaguagga
uguaugguug uuagauagac aacuacaauc uuuucucaca 60acagacag
68128387RNAhomo sapiens 1283aaaaaaaagg gaaagaagaa cuguugcauu
ugcccugcac ucaguuugca caggguaaau 60gcaauaguuc uucuuucccu uuuuuua
87128477RNAhomo sapiens 1284cucagcccgg gcaauauagu gagaccucgu
cucuacaaaa aauugagaca gggccucacu 60guaucgccca ggcugga
77128586RNAhomo sapiens 1285auucuaggug gggagacuga cggcuggagg
cccauaagcu gucuaaaacu ucggccccca 60gauuucuggu cuccccacuu cagaac
86128657RNAhomo sapiens 1286guugagacag gcaggauugg ggaaacaucu
uuuaccucgu cucuugccug uuuuaga 57128781RNAhomo sapiens
1287gcgggaggug uaacaggacu ggacucccgg cagccccagg gcaggggcgu
ggggagcugg 60uccuagcuca gcgcucccgg a 81128867RNAhomo sapiens
1288agcguuaccu gguagugagu uagagaugca gagcccuggg cuccucagca
aaccuacugg 60aucugca 67128976RNAhomo sapiens 1289ucugcagcuc
ccggcagccu cgggccacac ucccgggauc cccagggacu ggccugggac 60uaccgggggu
ggcggc 76129086RNAhomo sapiens 1290agggagaagg gucggggcag ggagggcagg
gcaggcucug gggugggggg ucugugaguc 60agccacggcu cugcccacgu cucccc
86129170RNAhomo sapiens 1291agcuuccaga cgcuccgccc cacgucgcau
gcgccccggg aacgcguggg gcggagcuuc 60cggaggcccc 70129270RNAhomo
sapiens 1292agcuuccaga cgcuccgccc cacgucgcau gcgccccggg aacgcguggg
gcggagcuuc 60cggaggcccc 70129370RNAhomo sapiens 1293agcuuccaga
cgcuccgccc cacgucgcau gcgccccggg aacgcguggg gcggagcuuc 60cggaggcccc
70129470RNAhomo sapiens 1294agcuuccaga cgcuccgccc cacgucgcau
gcgccccggg aacgcguggg gcggagcuuc 60cggaggcccc 70129570RNAhomo
sapiens 1295agcuuccaga cgcuccgccc cacgucgcau gcgccccggg aacgcguggg
gcggagcuuc 60cggaggcccc 70129684RNAhomo sapiens 1296uggagggcau
uaggcagugg ccagagcccu gcagugcugg gcaugggcuu cucgugggcu 60cuggccacgg
cccugagcuc cucc 84129788RNAhomo sapiens 1297gccaggauca cagacguuua
aauuacacuc cuucugcugu gccuuacagc aguagaaggg 60gugaaauuua aacgucugug
auccuggg 88129883RNAhomo sapiens 1298gaucacagac guuuaaauua
cacuccuucu gcugugccuu acagcaguag aaggggugaa 60auuuaaacgu cugugauccu
ggg 83129987RNAhomo sapiens 1299gccaggauca cagacguuua aauuacacuc
cuucugcugu gccuuacagc aguagaaggg 60gugaaauuua aacgucugug auccugg
87130079RNAhomo sapiens 1300agguaaauau gaugaaacuc acagcugagg
agcuuagcaa guagcuaagg ccagagcuug 60uguuugggug guguggcug
79130183RNAhomo sapiens 1301ugggggaaaa gugcugggau ugauuaguga
ugucugcugg ggaaccgggg cucagggaug 60auaacugugc ugagaagccc ccu
83130258RNAhomo sapiens 1302aaccucagca gugcgcaggg cugcacuguc
uccgucugcg gccugcagua agcgggua 58130370RNAhomo sapiens
1303gugugccacc ugcguguuuu cuguccaaau cagaaaagga uuuggacaga
aaacacgcag 60gaagaaggaa 70130460RNAhomo sapiens 1304ucggcuaagg
aaguccugug cucaguuuug uagcaucaaa acuaggauuu cucuuguuac
60130575RNAhomo sapiens 1305ugagguuucu ggacugagcc augcuacugg
cuucucuggu ucuccagcuu acagauggcu 60uaucauggga ccucu 75130687RNAhomo
sapiens 1306gcgggcguug ccugggggcc ucgcaggggg agauccagcc caggcugguu
ccgcugacuc 60ugccuguagg ccgguggcgu cuucugg 87130769RNAhomo sapiens
1307gcgggggacc gagagggccu cggcugugug aggacuagag gcggccgagg
cccgggccgg 60uucccccga 69130870RNAhomo sapiens 1308auuuuagagg
cuggaauaga gauucuugag gcuuggaaga guaaggaucc cuuuaucugu 60ccucuaggag
70130956RNAhomo sapiens 1309uggaucugaa ggcugccccu uugcucucug
ggguagccuu cagaucuugg uguuuu 56131065RNAhomo sapiens 1310ugggccacuu
ggaucugaag gcugccccuu ugcucucugg gguagccuuc agaucuuggu 60guuuu
65131169RNAhomo sapiens 1311gaacgauagc agcaugaacc ugucucacug
cagaauuauu uugagacagg cuuaugcugc 60uauccuuca 69131270RNAhomo
sapiens 1312ggaacgauag cagcaugaac cugucucacu gcagaauuau uuugagacag
gcuuaugcug 60cuauccuuca 70131375RNAhomo sapiens 1313gucagagggg
ggaugugcau gcugguuggg gugggcugcc uguggaccaa ucagcgugca 60cuuccccacc
cugaa 75131454RNAhomo sapiens 1314cuuagcuccc uggcuucagc ccuuuuucca
gggaggugaa ugguucuguc ucgc 54131587RNAhomo sapiens 1315ugcggugaca
ucagggccca gucccugcug ucaugcccca ggugacgugc ugggcugaca 60gcagggcugg
ccgcuaacgu cacuguc 87131670RNAhomo sapiens 1316ccagaagugg
ucugcaaaga gaugacugug aauccaagau ccacaucagc ucugugcugc 60cuacaucuga
70131790RNAhomo sapiens 1317uauucuacug agaguacaga ucuuuauaua
uaugaucauu auauguauga ugagaucauu 60auauguauga ucuggacacc caguagaauc
90131878RNAhomo sapiens 1318augacaggcc aucagcaguc caaugaagac
augaagaccc aaugucuuca uuggacugcu 60gauggcccgu cacuggga
78131970RNAhomo sapiens 1319cuccacauug gagggugugg aagacaucug
ggccaacucu gaucucuuca ucuacccccc 60aggacuggga 70132056RNAhomo
sapiens 1320cgaccgcacc cgcccgaagc ugggucaagg agcccagcag gacgggagcg
cggcgc 56132147RNAhomo sapiens 1321gccuaggagu ccuuggucag uggggacaug
gagaaggcuu cugagga 47132260RNAhomo sapiens 1322gccuucucuu
cccaguucuu ccuggagucg gggaaaagcu ggguugagaa ggugaaaaga
60132373RNAhomo sapiens 1323ggucaccugu cuggccagcu acguccccac
ggcccuuguc aguguggaag guagacggcc 60agagagguga ccc 73132479RNAhomo
sapiens 1324aggaagggau ucugggaggu uguagcagug gaaaaaguuc uuuucuuccu
cugaucgccc 60ucucagcucu uuccuucug 79132551RNAhomo sapiens
1325acagaccccg gggagcccgg cggugaagcu ccugguaucc ugggugucug a
51132671RNAhomo sapiens 1326ugagaaugug gaaggagguu gccggacgcu
gcuggcugcc uuccagcguc cacuucccuu 60ucucucucuc c 71132776RNAhomo
sapiens 1327cgguuggugc aaagguaauu gugguuucug ccauugaaag uaaaggcaag
aaccucaauu 60accuuugcag cgaccu 76132876RNAhomo sapiens
1328ucgagggaau cugagaaggc gcacaagguu uguguccaau acaguccaca
ccuugcgcua 60cucaggucug cucgug 76132975RNAhomo sapiens
1329ucgagggaau cugagaaggc gcacaagguu uguguccaau acaguccaca
ccuugcgcua 60cucaggucug cucgu 75133060RNAhomo sapiens
1330ggcugaagcu cuaagguucc gccugcgggc aggaagcgga ggaaccuugg
agcuucggca 60133160RNAhomo sapiens 1331ugugaaugac ccccuuccag
agccaaaauc accagggaug gaggaggggu cuuggguacu 60133276RNAhomo sapiens
1332gggagcacug gacuaggagu cagaaggugg aguucugggu gcuguuuucc
cacucuuggg 60cccugggcau guucug 76133359RNAhomo sapiens
1333aacugggucc cagucuucac aguugguuuc ugacacgugg accuggcugg
gacgaugug 59133461RNAhomo sapiens 1334ccaccuggac ccagcguaga
caaagaggug uuucuacucc auaucuaccu ggacccagug 60u 61133574RNAhomo
sapiens 1335aguuggugca aaaguaauug cgguuuuugc cgucgaaaau aauggcaaaa
acugcaguua 60cuuuuguacc aaug 74133653RNAhomo sapiens 1336uuugggcugg
gcuggguugg gcaguucuuc ugcuggacuc accugugacc agc 53133761RNAhomo
sapiens 1337auguguggua gauauaugca cuguauauaa acauaaugug guagauauau
gcacgauaua 60g 61133884RNAhomo sapiens 1338augguguuug ccuccuucau
ccgcaaggca ucugaugccc acgaaguuag gaagguccuu 60ggggagaagg gaaagaacau
caaa 84133969RNAhomo sapiens 1339cgcaugacuc uucaaccuca ggacuugcag
aauuaaugga augcuguccu aagguuguug 60aguugugca 69134083RNAhomo
sapiens 1340cauuagguug gugcaaaagg cauugugguu uuugccuaua aaaguaaugg
caaaaaccgc 60aauuccuuuu gcaccaaccu aau 83134161RNAhomo sapiens
1341cacucgcgcu gcggccagcg cccgggccug cgggcccggg cggcggcugu
guugcgcagu 60c 61134283RNAhomo sapiens 1342uuauuaauau ggaagggaga
agagcuuuaa ugauuggagu cauuuucaga gcauuaaagc 60ucuucucccu uccauauuaa
ugu 83134366RNAhomo sapiens 1343ugccgcggcg gggacggcga uugguccgua
uguguggugc caccggccgc cggcuccgcc 60ccggcc 66134471RNAhomo sapiens
1344gcaaagggug gcagcaagga aggcaggggu ccuaaggugu guccuccugc
ccuccuugcu 60guagacuuug g 71134570RNAhomo sapiens 1345ugagccgagc
ugagcuuagc ugggcugagc uaaccagggc ugggcugagc ugggcugagc 60ugagcugagc
70134678RNAhomo sapiens 1346gagcuuggau gagcugggcu gaacugggcu
ggguugagcu gggcugggcu gaguugagcc 60aggcugaucu gggcugag
78134760RNAhomo sapiens 1347ugagcugggc ucugcugugc ugugcugagc
agggcugagc ugaacugggc ugagcugggc 60134861RNAhomo sapiens
1348gucaagucag aacagccagg uagagcccuu guccaaaccu gggcugaaug
acagugauga 60g 611349104RNAhomo sapiens 1349cucuuugagc cuuggcugcc
uuggugcagc agggucaucu guagggccac cccacagcuc 60uuuccuuccc cuccucucuc
cagggagccg gggcugugag agga 104135055RNAhomo sapiens 1350aagcugcaug
gaccaggacu uggcaccuuu ggccuuaguc cugccuguag guuua
55135122DNAArtificialsynthetic primer 1351aagcccttac cccaaaaagc at
22135221DNAArtificialsynthetic primer 1352aggcggagac ttgggcaatt g
21135322DNAArtificialsynthetic primer 1353ctgggaggtg gatgtttact tc
22135422DNAArtificialsynthetic primer 1354ctgggagaag gctgtttact ct
22135522DNAArtificialsynthetic primer 1355ctttcagtcg gatgtttaca gc
22135623DNAArtificialsynthetic primer 1356tctcacacag aaatcgcacc cgt
23135722DNAArtificialsynthetic primer 1357tgaggtagta ggttgtatag tt
22135822DNAArtificialsynthetic primer 1358tgaggtagta ggttgtatag tt
22135922DNAArtificialsynthetic primer 1359tgaggtagta ggttgtatgg tt
22136022DNAArtificialsynthetic primer 1360aacccgtaga tccgaacttg tg
22136121DNAArtificialsynthetic primer 1361tacagtactg tgataactga a
21136221DNAArtificialsynthetic primer 1362tacagtactg tgataactga a
21136323DNAArtificialsynthetic primer 1363agcagcattg tacagggcta tga
23136423DNAArtificialsynthetic primer 1364agcagcattg tacagggcta tga
23136523DNAArtificialsynthetic primer 1365aaaagtgctt acagtgcagg tag
23136621DNAArtificialsynthetic primer 1366taaagtgctg acagtgcaga t
21136723DNAArtificialsynthetic primer 1367agcagcattg tacagggcta tca
23136823DNAArtificialsynthetic primer 1368taccctgtag atccgaattt gtg
23136923DNAArtificialsynthetic primer 1369taccctgtag aaccgaattt gtg
23137021DNAArtificialsynthetic primer 1370tcacagtgaa ccggtctctt t
21137121DNAArtificialsynthetic primer 1371tcacagtgaa ccggtctctt t
21137221DNAArtificialsynthetic primer 1372ctttttgcgg tctgggcttg c
21137321DNAArtificialsynthetic primer 1373ctttttgcgg tctgggcttg c
21137421DNAArtificialsynthetic primer 1374ttaggccgca gatctgggtg a
21137521DNAArtificialsynthetic primer 1375taccacaggg tagaaccacg g
21137621DNAArtificialsynthetic primer 1376tgagatgaag cactgtagct c
21137722DNAArtificialsynthetic primer 1377tgagaactga attccatggg tt
22137822DNAArtificialsynthetic primer 1378tgagaactga attccatagg ct
22137922DNAArtificialsynthetic primer 1379tcagtgcact acagaacttt gt
22138021DNAArtificialsynthetic primer 1380ctagactgaa gctccttgag g
21138121DNAArtificialsynthetic primer 1381tcagtgcatg acagaacttg g
21138223DNAArtificialsynthetic primer 1382ttaatgctaa tcgtgatagg ggt
23138322DNAArtificialsynthetic primer 1383tagcagcaca taatggtttg tg
22138422DNAArtificialsynthetic primer 1384tagcagcaca tcatggttta ca
22138522DNAArtificialsynthetic primer 1385tagcagcacg taaatattgg cg
22138622DNAArtificialsynthetic primer 1386tagcagcacg taaatattgg cg
22138723DNAArtificialsynthetic primer 1387caaagtgctt acagtgcagg tag
23138823DNAArtificialsynthetic primer 1388aacattcaac gctgtcggtg agt
23138923DNAArtificialsynthetic primer 1389aacattcaac gctgtcggtg agt
23139022DNAArtificialsynthetic primer 1390aacattcatt gctgtcggtg gg
22139122DNAArtificialsynthetic primer 1391aacattcatt gctgtcggtg gg
22139222DNAArtificialsynthetic primer 1392aacattcaac ctgtcggtga gt
22139324DNAArtificialsynthetic primer 1393aacattcatt gttgtcggtg
ggtt 24139422DNAArtificialsynthetic primer 1394tggagagaaa
ggcagttcct ga 22139523DNAArtificialsynthetic primer 1395taaggtgcat
ctagtgcaga tag 23139623DNAArtificialsynthetic primer 1396caacggaatc
ccaaaagcag ctg 23139721DNAArtificialsynthetic primer 1397ctgacctatg
aattgacagc c 21139822DNAArtificialsynthetic primer 1398tgggtctttg
cgggcgagat ga 22139922DNAArtificialsynthetic primer 1399taggtagttt
cctgttgttg gg 22140022DNAArtificialsynthetic primer 1400ttcaccacct
tctccaccca gc 22140122DNAArtificialsynthetic primer 1401acagtagtct
gcacattggt ta 22140223DNAArtificialsynthetic primer 1402tgtgcaaatc
tatgcaaaac tga 23140323DNAArtificialsynthetic primer 1403taaagtgctt
atagtgcagg tag 23140423DNAArtificialsynthetic primer 1404caaagtgctc
atagtgcagg tag 23140523DNAArtificialsynthetic primer 1405agctacattg
tctgctgggt ttc 23140621DNAArtificialsynthetic primer 1406agctacatct
ggctactggg t 21140721DNAArtificialsynthetic primer 1407atcacattgc
cagggatttc c 21140822DNAArtificialsynthetic primer 1408cattgcactt
gtctcggtct ga 22140922DNAArtificialsynthetic primer 1409ttcaagtaat
ccaggatagg ct 22141022DNAArtificialsynthetic primer 1410ttcaagtaat
ccaggatagg ct 22141121DNAArtificialsynthetic primer 1411ttcaagtaat
tcaggatagg t 21141221DNAArtificialsynthetic primer 1412ttcacagtgg
ctaagttccg c 21141322DNAArtificialsynthetic primer 1413tagcaccatc
tgaaatcggt ta 22141422DNAArtificialsynthetic primer 1414tgtaaacatc
ctcgactgga ag 22141522DNAArtificialsynthetic primer 1415tgtaaacatc
ctacactcag ct 22141623DNAArtificialsynthetic primer 1416tgtaaacatc
ctacactctc agc 23141723DNAArtificialsynthetic primer 1417tgtaaacatc
ctacactctc agc 23141822DNAArtificialsynthetic primer 1418tgtaaacatc
cccgactgga ag 22141922DNAArtificialsynthetic primer 1419tgtaaacatc
cttgactgga ag 22142022DNAArtificialsynthetic primer 1420aaaagctggg
ttgagagggc ga 22142122DNAArtificialsynthetic primer 1421aaaagctggg
ttgagagggc aa 22142222DNAArtificialsynthetic primer 1422aaaagctggg
ttgagagggc aa 22142321DNAArtificialsynthetic primer 1423gcccctgggc
ctatcctaga a 21142421DNAArtificialsynthetic primer 1424gtgcattgta
gttgcattgc a 21142522DNAArtificialsynthetic primer 1425ttataaagca
atgagactga tt 22142622DNAArtificialsynthetic primer 1426tggcagtgtc
ttagctggtt gt 22142722DNAArtificialsynthetic primer 1427ttatcagaat
ctccaggggt ac 22142822DNAArtificialsynthetic primer 1428aattgcacgg
tatccatctg ta 22142922DNAArtificialsynthetic primer 1429taatgcccct
aaaaatcctt at 22143022DNAArtificialsynthetic primer 1430ttataataca
acctgataag tg 22143121DNAArtificialsynthetic primer 1431actggacttg
gagtcagaag g 21143223DNAArtificialsynthetic primer 1432tgaggggcag
agagcgagac ttt 23143322DNAArtificialsynthetic primer 1433tggcagtgta
ttgttagctg gt 22143423DNAArtificialsynthetic primer 1434tagcagcggg
aacagttctg cag 23143522DNAArtificialsynthetic primer 1435catgccttga
gtgtaggacc gt 22143622DNAArtificialsynthetic primer 1436aagatgtgga
aaaattggaa tc 22143721DNAArtificialsynthetic primer 1437agggggaaag
ttctatagtc c 21143823DNAArtificialsynthetic primer 1438tggaagacta
gtgattttgt tgt 23143923DNAArtificialsynthetic primer 1439tggaagacta
gtgattttgt tgt 23144023DNAArtificialsynthetic primer 1440tggaagacta
gtgattttgt tgt 23144122DNAArtificialsynthetic primer 1441ctgccctggc
ccgagggacc ga 22144223DNAArtificialsynthetic primer 1442tctttggtta
tctagctgta tga 23144323DNAArtificialsynthetic primer 1443tctttggtta
tctagctgta tga 23144422DNAArtificialsynthetic primer 1444tattgcactt
gtcccggcct gt 22144522DNAArtificialsynthetic primer 1445tattgcactt
gtcccggcct gt 22144623DNAArtificialsynthetic primer 1446caaagtgctg
ttcgtgcagg tag 23144723DNAArtificialsynthetic primer 1447tctttggtta
tctagctgta tga 23144822DNAArtificialsynthetic primer 1448aacccgtaga
tccgatcttg tg 22144923DNAArtificialsynthetic primer 1449aaaggtaatt
gcagtttttc cca 23145019DNAArtificialsynthetic primer 1450actggacttg
gagtcagga 19145124DNAArtificialsynthetic primer 1451aggcagtgta
ttgctagcgg ctgt 24145222DNAArtificialsynthetic primer
1452tgcacccagg caaggattct gc 22145317DNAArtificialsynthetic primer
1453tgagggagga gactgca 17145420DNAArtificialsynthetic primer
1454actggacttg gagccagaag 20145526DNAArtificialsynthetic primer
1455gtcactgatg tctgtagctg agacgg 26145623DNAArtificialsynthetic
primer 1456gatgaggatg gatagcaagg aag 23145721DNAArtificialsynthetic
primer 1457aaaagcatca ggaagtaccc a 21145827DNAArtificialsynthetic
primer 1458gtcaaatgaa gggctgatca cgaaata
27145922DNAArtificialsynthetic primer 1459agagttaact caaaatggac ta
22146023DNAArtificialsynthetic primer 1460tgttgggatt cagcaggacc att
23146122DNAArtificialsynthetic primer 1461taaatagagt aggcaaagga ca
22146226DNAArtificialsynthetic primer 1462aagaggaaga aatggctggt
tctcag 26146322DNAArtificialsynthetic primer 1463tagtggatga
tgcactctgt gc 22146420DNAArtificialsynthetic primer 1464aaagactctg
caagatgcct 20146518DNAArtificialsynthetic primer 1465aggagaagta
aagtagaa 18146622DNAArtificialsynthetic primer 1466atggccagag
ctcacacaga gg 22146723DNAArtificialsynthetic primer 1467atcagggctt
gtggaatggg aag 23146822DNAArtificialsynthetic primer 1468atggccagag
ctcacacaga gg 22146923DNAArtificialsynthetic primer 1469tgaggatatg
gcagggaagg gga 23147022DNAArtificialsynthetic primer 1470tgggctcagg
gtacaaaggt tc 22147121DNAArtificialsynthetic primer 1471gctgcaccgg
agactgggta a 21147221DNAArtificialsynthetic primer 1472gctgcaccgg
agactgggta a 21147322DNAArtificialsynthetic primer 1473tgtcgtgggg
cttgctggct tg 22147419DNAArtificialsynthetic
primer 1474actggacttg gaggcagaa 19147517DNAArtificialsynthetic
primer 1475ttggaggcgt gggtttt 17147622DNAArtificialsynthetic primer
1476ctgactgaat aggtagggtc at 22147722DNAArtificialsynthetic primer
1477agattgtttc ttttgccgtg ca 22147821DNAArtificialsynthetic primer
1478cacggcaaaa gaaacaatcc a 21147922DNAArtificialsynthetic primer
1479cagggctggc agtgacatgg gt 22148017DNAArtificialsynthetic primer
1480ggtgggggct gttgttt 17148123DNAArtificialsynthetic primer
1481tggggaggtg tggagtcagc atg 23148220DNAArtificialsynthetic primer
1482ggctccttgg tctaggggta 20148322DNAArtificialsynthetic primer
1483tggggatttg gagaagtggt ga 22148422DNAArtificialsynthetic primer
1484aaaagtgatt gcagtgtttg cc 22148522DNAArtificialsynthetic primer
1485taggagctca acagatgcct gt 22148621DNAArtificialsynthetic primer
1486agcttttggg aattcaggta g 21148721DNAArtificialsynthetic primer
1487caaaagtgat cgtggttttt g 21148817DNAArtificialsynthetic primer
1488agggtgtgtg tgttttt 17148921DNAArtificialsynthetic primer
1489actgacagga gagcattttg a 21149023DNAArtificialsynthetic primer
1490atagtggttg tgaatttacc ttc 23149124DNAArtificialsynthetic primer
1491ctaccccagg atgccagcat agtt 24149221DNAArtificialsynthetic
primer 1492actggacttg gtgtcagatg g 21149322DNAArtificialsynthetic
primer 1493tagtggatga tggagactcg gt 22149421DNAArtificialsynthetic
primer 1494aaggtttgga tagatgcaat a 21149523DNAArtificialsynthetic
primer 1495aaaggtaatt gcagtttttc cca 23149622DNAArtificialsynthetic
primer 1496aggggaccaa agagatatat ag 22149722DNAArtificialsynthetic
primer 1497ggcgacaaaa cgagaccctg tc 22149819DNAArtificialsynthetic
primer 1498gggtgcgggc cggcggggt 19149922DNAArtificialsynthetic
primer 1499catgctagga tagaaagaat gg 22150022DNAArtificialsynthetic
primer 1500gcaaagtgat gagtaatact gg 22150121DNAArtificialsynthetic
primer 1501ccctggggtt ctgaggacat g 21150222DNAArtificialsynthetic
primer 1502caggaaggat ttagggacag gc 22150322DNAArtificialsynthetic
primer 1503attaaggaca tttgtgattg at 22150421DNAArtificialsynthetic
primer 1504aaaaggcata aaaccaagac a 21150521DNAArtificialsynthetic
primer 1505aaaaggcata aaaccaagac a 21150620DNAArtificialsynthetic
primer 1506taaaaactgc aattactttc 20150722DNAArtificialsynthetic
primer 1507tgtgatatca tggttcctgg ga 22150822DNAArtificialsynthetic
primer 1508tgtgatatca tggttcctgg ga 22150922DNAArtificialsynthetic
primer 1509tgtgatatca tggttcctgg ga 22151022DNAArtificialsynthetic
primer 1510tgtgatatcg tgcttcctgg ga 22151121DNAArtificialsynthetic
primer 1511aaaagtaact gcggtttttg a 21151217DNAArtificialsynthetic
primer 1512ggagtgggct ggtggtt 17151322DNAArtificialsynthetic primer
1513aagggcttcc tctctgcagg ac 22151422DNAArtificialsynthetic primer
1514aagggcttcc tctctgcagg ac 22151519DNAArtificialsynthetic primer
1515agagctggct gaagggcag 19151622DNAArtificialsynthetic primer
1516tgtgacttta agggaaatgg cg 22151722DNAArtificialsynthetic primer
1517tctcaggagt aaagacagag tt 22151822DNAArtificialsynthetic primer
1518aggtggatgc aatgtgacct ca 22151918DNAArtificialsynthetic primer
1519aggctgggct gggacgga 18152020DNAArtificialsynthetic primer
1520taggatgggg gtgagaggtg 20152122DNAArtificialsynthetic primer
1521tgagggagta ggatgtatgg tt 22152222DNAArtificialsynthetic primer
1522agactgacgg ctggaggccc at 22152322DNAArtificialsynthetic primer
1523tagtgagtta gagatgcaga gc 22152417DNAArtificialsynthetic primer
1524gggagaaggg tcggggc 17152522DNAArtificialsynthetic primer
1525ctcgtgggct ctggccacgg cc 22152622DNAArtificialsynthetic primer
1526agaaggggtg aaatttaaac gt 22152722DNAArtificialsynthetic primer
1527agaaggggtg aaatttaaac gt 22152822DNAArtificialsynthetic primer
1528agaaggggtg aaatttaaac gt 22152926DNAArtificialsynthetic primer
1529gctcagggat gataactgtg ctgaga 26153022DNAArtificialsynthetic
primer 1530ctggactgag ccatgctact gg 22153122DNAArtificialsynthetic
primer 1531atagcagcat gaacctgtct ca 22153222DNAArtificialsynthetic
primer 1532tgagacaggc ttatgctgct at 22153323DNAArtificialsynthetic
primer 1533tggtctgcaa agagatgact gtg 23153421DNAArtificialsynthetic
primer 1534ttggagggtg tggaagacat c 21153517DNAArtificialsynthetic
primer 1535atggagaagg cttctga 17153618DNAArtificialsynthetic primer
1536aaaagctggg ttgagaag 18153722DNAArtificialsynthetic primer
1537tggaaggtag acggccagag ag 22153822DNAArtificialsynthetic primer
1538tctgggaggt tgtagcagtg ga 22153923DNAArtificialsynthetic primer
1539ggaggaacct tggagcttcg gca 23154021DNAArtificialsynthetic primer
1540actggactag gagtcagaag g 21154122DNAArtificialsynthetic primer
1541caaaaactgc agttactttt gt 22154220DNAArtificialsynthetic primer
1542aaaaggcatt gtggtttttg 20154334DNAArtificialsynthetic primer
1543atatctcgag gcccgagtcc ccgggcatct ttgg
34154432DNAArtificialsynthetic primer 1544atatatctag actacacacg
acaaatactt tg 32154541DNAArtificialsynthetic primer 1545cagcccatcc
atagtaacag tcatgattag cagaagaaag g 41154641DNAArtificialsynthetic
primer 1546cctttcttct gctaatcatg actgttacta tggatgggct g
41154732DNAArtificialsynthetic primer 1547atatctcgag ggtcacatct
cccaggaaga tc 32154833DNAArtificialsynthetic primer 1548atatatctag
aagcactctc atggtgtgtg tag 33154933DNAArtificialsynthetic primer
1549agagactcga ggattttcag aaaacactta ttt
33155021DNAArtificialsynthetic primer 1550ttgcttctct agaggagaaa c
21155121DNAArtificialsynthetic primer 1551gtttctcctc tagagaagca a
21155235DNAArtificialsynthetic primer 1552agagagcggc cgcaggggag
agacaaattg cattg 35155331DNAArtificialsynthetic primer
1553agagactcga gataaaagga ggaatcttaa g
31155435DNAArtificialsynthetic primer 1554agagagcggc cgcgaaaaaa
acaaaacaaa aacaa 35155533DNAArtificialsynthetic primer
1555agagagcggc cgcccgattg agtcttgcct cat
33155631DNAArtificialsynthetic primer 1556agagagaatt caatggtctc
acatttccaa c 31155733DNAArtificialsynthetic primer 1557agagagcggc
cgcatgtcac agctattgtt cag 33155831DNAArtificialsynthetic primer
1558agagagaatt cgcagtaaaa gaatgcagct a
31155940DNAArtificialsynthetic primer 1559ggaagcgagt tgttatctat
gcttatctag ctgtatgagt 40156040DNAArtificialsynthetic primer
1560actcatacag ctagataagc atagataaca actcgcttcc
40156140DNAArtificialsynthetic primer 1561accaagtttc agttcatgtt
aagatcctac actcagctgt 40156240DNAArtificialsynthetic primer
1562acagctgagt gtaggatctt aacatgaact gaaacttggt
40156340DNAArtificialsynthetic primer 1563cagaaagtct gttgttgtta
agatccccga ctggaagctg 40156440DNAArtificialsynthetic primer
1564cagcttccag tcggggatct taacaacaac agactttctg 40156523RNAhomo
sapiens 1uggucugcaa agagaugacu gug 23
* * * * *
References