U.S. patent application number 13/477843 was filed with the patent office on 2012-10-25 for human transmembrane proteins.
This patent application is currently assigned to INCYTE CORPORATION. Invention is credited to Janice Au-Young, Olga Bandman, Mariah R. Baughn, Neil C. Corley, Gina A. Gorgone, Karl J. Guegler, Jennifer L. Hillman, Matthew R. Kaser, Preeti Lal, Chandra Patterson, Y. Tom Tang, Henry Yue.
Application Number | 20120270809 13/477843 |
Document ID | / |
Family ID | 27492120 |
Filed Date | 2012-10-25 |
United States Patent
Application |
20120270809 |
Kind Code |
A1 |
Tang; Y. Tom ; et
al. |
October 25, 2012 |
HUMAN TRANSMEMBRANE PROTEINS
Abstract
The invention provides human transmembrane proteins (HTMPN) and
polynucleotides which identify and encode HTMPN. The invention also
provides expression vectors, host cells, antibodies, agonists, and
antagonists. The invention also provides methods for diagnosing,
treating, or preventing disorders associated with expression of
HTMPN.
Inventors: |
Tang; Y. Tom; (San Jose,
CA) ; Lal; Preeti; (Santa Clara, CA) ;
Hillman; Jennifer L.; (Mountain View, CA) ; Yue;
Henry; (Sunnyvale, CA) ; Guegler; Karl J.;
(Menlo Park, CA) ; Corley; Neil C.; (Castro
Valley, CA) ; Bandman; Olga; (Mountain View, CA)
; Patterson; Chandra; (Menlo Park, CA) ; Gorgone;
Gina A.; (Boulder Creek, CA) ; Kaser; Matthew R.;
(Castro Valley, CA) ; Baughn; Mariah R.; (Los
Angeles, CA) ; Au-Young; Janice; (Brisbane,
CA) |
Assignee: |
INCYTE CORPORATION
|
Family ID: |
27492120 |
Appl. No.: |
13/477843 |
Filed: |
May 22, 2012 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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11594148 |
Nov 8, 2006 |
8206947 |
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13477843 |
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09700590 |
Apr 16, 2001 |
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PCT/US99/11904 |
May 28, 1999 |
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11594148 |
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60087260 |
May 29, 1998 |
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60091674 |
Jul 2, 1998 |
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60102954 |
Oct 2, 1998 |
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60109869 |
Nov 24, 1998 |
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Current U.S.
Class: |
514/21.2 ;
435/7.9; 436/501; 530/350; 530/387.9; 530/391.3 |
Current CPC
Class: |
A61P 7/06 20180101; A61P
25/14 20180101; A61K 38/00 20130101; A61P 5/24 20180101; A61P 17/06
20180101; A61P 25/00 20180101; A61P 1/04 20180101; A61P 25/06
20180101; A61P 35/00 20180101; C07K 14/705 20130101; A61P 25/16
20180101; G01N 33/68 20130101; A61P 1/16 20180101; A61P 15/10
20180101; A61P 19/02 20180101; A61P 7/02 20180101; A61P 31/10
20180101; A61P 31/12 20180101; C07K 2317/24 20130101; C07K 2317/622
20130101; A61P 1/12 20180101; A61P 25/28 20180101; A61P 3/10
20180101; A61P 15/08 20180101; A61P 31/04 20180101; A61P 1/10
20180101; A61P 11/06 20180101; A61P 9/10 20180101; A61P 33/00
20180101; A61P 1/08 20180101; A61P 37/02 20180101; C07K 2317/76
20130101; C07K 16/28 20130101 |
Class at
Publication: |
514/21.2 ;
530/350; 530/387.9; 530/391.3; 436/501; 435/7.9 |
International
Class: |
C07K 14/435 20060101
C07K014/435; G01N 33/566 20060101 G01N033/566; C07K 17/00 20060101
C07K017/00; A61K 38/17 20060101 A61K038/17; C07K 16/18 20060101
C07K016/18 |
Claims
1.-20. (canceled)
21. An isolated polypeptide comprising an amino acid sequence
having at least about 90% sequence identity to SEQ ID NO: 74.
22. An isolated polypeptide comprising an amino acid sequence
having at least about 95% sequence identity to SEQ ID NO: 74.
23. The isolated polypeptide of claim 21 comprising SEQ ID NO:
74.
24. The isolated peptide of claim 21 which has been modified by one
or more of acetylation, carboxylation, glycosylation,
phosphorylation, lipidation, or acylation.
25. The isolated peptide of claim 21 which is ligated to a
heterologous amino acid sequence.
26. A composition comprising the polypeptide of claim 21 and at
least one pharmaceutically acceptable excipient.
27. A method of screening a compound for effectiveness as an
agonist of the polypeptide of claim 21, the method comprising: (a)
exposing a sample comprising the polypeptide of claim 21 to the
compound, and (b) detecting agonist activity in the sample.
28. A method of screening a compound for effectiveness as an
antagonist of the polypeptide of claim 21, the method comprising:
(a) exposing a sample comprising the polypeptide of claim 21 to the
compound, and (b) detecting antagonist activity in the sample.
29. A method of screening for a compound that specifically binds to
the polypeptide of claim 21, the method comprising: (a) combining
the polypeptide of claim 21 with at least one test compound under
suitable conditions, and (b) detecting binding of the polypeptide
of claim 21 to the test compound, thereby identifying a compound
that specifically binds to the polypeptide of claim 21.
30. An antibody that specifically binds to a polypeptide comprising
SEQ ID NO: 74.
31. The antibody of claim 28 which is labeled.
32. The antibody of claim 29, wherein the label is selected from
the group consisting of radionuclides, enzymes, fluorescent,
chemiluminescent, and chromogenic agents.
33. The antibody of claim 30 which is monoclonal.
Description
TECHNICAL FIELD
[0001] This invention relates to nucleic acid and amino acid
sequences of human transmembrane proteins and to the use of these
sequences in the diagnosis, treatment, and prevention of immune,
reproductive, smooth muscle, neurological, gastrointestinal,
developmental, and cell proliferative disorders.
BACKGROUND OF THE INVENTION
[0002] Eukaryotic organisms are distinct from prokaryotes in
possessing many intracellular organelle and vesicle structures.
Many of the metabolic reactions which distinguish eukaryotic
biochemistry from prokaryotic biochemistry take place within these
structures. In particular, many cellular functions require very
stringent reaction conditions, and the organelles and vesicles
enable compartmentalization and isolation of reactions which might
otherwise disrupt cytosolic metabolic processes. The organelles
include mitochondria, smooth and rough endoplasmic reticula,
sarcoplasmic reticulum, and the Golgi body. The vesicles include
phagosomes, lysosomes, endosomes, peroxisomes, and secretory
vesicles. Organelles and vesicles are bounded by single or double
membranes.
[0003] Biological membranes are highly selective permeable barriers
made up of lipid bilayer sheets composed of phosphoglycerides,
fatty acids, cholesterol, phospholipids, glycolipids,
proteoglycans, and proteins. Membranes contain ion pumps, ion
channels, and specific receptors for external stimuli which
transmit biochemical signals across the membranes. These membranes
also contain second messenger proteins which interact with these
pumps, channels, and receptors to amplify and regulate transmission
of these signals.
Plasma Membrane Proteins
[0004] Plasma membrane proteins (MPs) are divided into two groups
based upon methods of protein extraction from the membrane.
Extrinsic or peripheral membrane proteins can be released using
extremes of ionic strength or pH, urea, or other disruptors of
protein interactions. Intrinsic or integral membrane proteins are
released only when the lipid bilayer of the membrane is dissolved
by detergent.
[0005] Transmembrane proteins (TM) are characterized by an
extracellular, a transmembrane, and an intracellular domain. TM
domains are typically comprised of 15 to 25 hydrophobic amino acids
which are predicted to adopt an .alpha.-helical conformation. TM
proteins are classified as bitopic (Types I and II) proteins, which
span the membrane once, and polytopic (Types III and IV) (Singer,
S. J. (1990) Annu. Rev. Cell Biol. 6:247-96) proteins which contain
multiple membrane-spanning segments. TM proteins that act as
cell-surface receptor proteins involved in signal transduction
include growth and differentiation factor receptors, and
receptor-interacting proteins such as Drosophila pecanex and
frizzled proteins, LIV-1 protein, NF2 protein, and GNS1/SUR4
eukaryotic integral membrane proteins. TM proteins also act as
transporters of ions or metabolites, such as gap junction channels
(connexins), and ion channels, and as cell anchoring proteins, such
as lectins, integrins, and fibronectins. TM proteins are found in
vesicle organelle-forming molecules, such as calveolins; or cell
recognition molecules, such as cluster of differentiation (CD)
antigens, glycoproteins, and mucins.
[0006] Many membrane proteins (MPs) contain amino acid sequence
motifs that serve to localize proteins to specific subcellular
sites. Examples of these motifs include PDZ domains, KDEL, RGD,
NGR, and GSL sequence motifs, von Willebrand factor A (vWFA)
domains, and EGF-like domains. RGD, NGR, and GSL motif-containing
peptides have been used as drug delivery agents in targeted cancer
treatment of tumor vasculature (Arap, W. et al. (1998) Science,
279:377-380). Membrane proteins may also contain amino acid
sequence motifs that serve to interact with extracellular or
intracellular molecules, such as carbohydrate recognition
domains.
[0007] Chemical modification of amino acid residue side chains
alters the manner in which MPs interact with other molecules, for
example, phospholipid membranes. Examples of such chemical
modifications to amino acid residue side chains are covalent bond
formation with glycosaminoglycans, oligosaccharides, phospholipids,
acetyl and palmitoyl moieties, ADP-ribose, phosphate, and sulphate
groups.
[0008] RNA-encoding membrane proteins may have alternative splice
sites which give rise to proteins encoded by the same gene but with
different messenger RNA and amino acid sequences. Splice variant
membrane proteins may interact with other ligand and protein
isoforms.
G-Protein Coupled Receptors
[0009] G-protein coupled receptors (GPCR) are a superfamily of
integral membrane proteins which transduce extracellular signals.
GPCRs include receptors for biogenic amines, lipid mediators of
inflammation, peptide hormones, and sensory signal mediators.
[0010] The structure of these highly-conserved receptors consists
of seven hydrophobic transmembrane (serpentine) regions, cysteine
disulfide bridges between the second and third extracellular loops,
an extracellular N-terminus, and a cytoplasmic C-terminus. Three
extracellular loops alternate with three intracellular loops to
link the seven transmembrane regions. The most conserved parts of
these proteins are the transmembrane regions and the first two
cytoplasmic loops. A conserved, acidic-Arg-aromatic residue triplet
present in the second cytoplasmic loop may interact with G
proteins. A GPCR consensus pattern is characteristic of most
proteins belonging to this superfamily (ExPASy PROSITE document
PS00237; and Watson, S, and S. Arkinstall (1994) The G-protein
Linked Receptor Facts Book, Academic Press, San Diego, Calif., pp
2-6). Mutations and changes in transcriptional activation of
GPCR-encoding genes have been associated with neurological
disorders such as schizophrenia, Parkinson's disease, Alzheimer's
disease, drug addiction, and feeding disorders.
Scavenger Receptors
[0011] Macrophage scavenger receptors with broad ligand specificity
may participate in the binding of low density lipoproteins (LDL)
and foreign antigens. Scavenger receptors types I and II are
trimeric membrane proteins with each subunit containing a small
N-terminal intracellular domain, a transmembrane domain, a large
extracellular domain, and a C-terminal cysteine-rich domain. The
extracellular domain contains a short spacer domain, an
.alpha.-helical coiled-coil domain, and a triple helical
collagenous domain. These receptors have been shown to bind a
spectrum of ligands, including chemically modified lipoproteins and
albumin, polyribonucleotides, polysaccharides, phospholipids, and
asbestos (Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci.
87:9133-9137; and Elomaa, O. et al. (1995) Cell 80:603-609). The
scavenger receptors are thought to play a key role in atherogenesis
by mediating uptake of modified LDL in arterial walls, and in host
defense by binding bacterial endotoxins, bacteria, and
protozoa.
Tetraspan Family Proteins
[0012] The transmembrane 4 superfamily (TM4SF) or tetraspan family
is a multigene family encoding type III integral membrane proteins
(Wright, M. D. and Tomlinson, M. G. (1994) Immunol. Today 15:588).
TM4SF is comprised of membrane proteins which traverse the cell
membrane four times. Members of the TM4SF include platelet and
endothelial cell membrane proteins, melanoma-associated antigens,
leukocyte surface glycoproteins, colonal carcinoma antigens,
tumor-associated antigens, and surface proteins of the schistosome
parasites (Jankowski, S. A. (1994) Oncogene 9:1205-1211). Members
of the TM4SF share about 25-30% amino acid sequence identity with
one another.
[0013] A number of TM4SF members have been implicated in signal
transduction, control of cell adhesion, regulation of cell growth
and proliferation, including development and oncogenesis, and cell
motility, including tumor cell metastasis. Expression of TM4SF
proteins is associated with a variety of tumors and the level of
expression may be altered when cells are growing or activated.
Tumor Antigens
[0014] Tumor antigens are surface molecules that are differentially
expressed in tumor cells relative to normal cells. Tumor antigens
distinguish tumor cells immunologically from normal cells and
provide diagnostic and therapeutic targets for human cancers
(Takagi, S. et al. (1995) Int. J. Cancer 61: 706-715; Liu, E. et
al. (1992) Oncogene 7: 1027-1032).
Ion Channels
[0015] Ion channels are found in the plasma membranes of virtually
every cell in the body. For example, chloride channels mediate a
variety of cellular functions including regulation of membrane
potentials and absorption and secretion of ions across epithelial
membranes. When present in intracellular membranes of the Golgi
apparatus and endocytic vesicles, chloride channels also regulate
organelle pH (see, e.g., Greger, R. (1988) Arum. Rev. Physiol.
50:111-122). Electrophysiological and pharmacological properties of
chloride channels, including ion conductance, current-voltage
relationships, and sensitivity to modulators, suggest that
different chloride channels exist in muscles, neurons, fibroblasts,
epithelial cells, and lymphocytes.
[0016] Many channels have sites for phosphorylation by one or more
protein kinases including protein kinase A, protein kinase C,
tyrosine kinase, and casein kinase II, all of which regulate ion
channel activity in cells. Inappropriate phosphorylation of
proteins in cells has been linked to changes in cell cycle
progression and cell differentiation. Changes in the cell cycle
have been linked to induction of apoptosis or cancer. Changes in
cell differentiation have been linked to diseases and disorders of
the reproductive system, immune system, and skeletal muscle.
Proton Pumps
[0017] Proton ATPases are a large class of membrane proteins that
use the energy of ATP hydrolysis to generate an electrochemical
proton gradient across a membrane. The resultant gradient may be
used to transport other ions across the membrane (Na.sup.+,
K.sup.+, or Cl.sup.-) or to maintain organelle pH. Proton ATPases
are further subdivided into the mitochondrial F-ATPases, the plasma
membrane ATPases, and the vacuolar ATPases. The vacuolar ATPases
establish and maintain an acidic pH within various vesicles
involved in the processes of endocytosis and exocytosis (Mellman,
I. et al. (1986) Ann. Rev. Biochem. 55:663-700).
[0018] Proton-coupled, 12 membrane-spanning domain transporters
such as PEPT 1 and PEPT 2 are responsible for gastrointestinal
absorption and for renal reabsorbtion of peptides using an
electrochemical H.sup..+-. gradient as the driving force. Another
type of peptide transporter, the TAP transporter, is a heterodimer
consisting of TAP 1 and TAP 2 and is associated with antigen
processing. Peptide antigens are transported across the membrane of
the endoplasmic reticulum by TAP so they can be expressed on the
cell surface in association with MHC molecules. Each TAP protein
consists of multiple hydrophobic membrane spanning segments and a
highly conserved ATP-binding cassette (Boll, M. et al. (1996) Proc.
Natl. Acad. Sci. 93:284-289). Pathogenic microorganisms, such as
herpes simplex virus, may encode inhibitors of TAP-mediated peptide
transport in order to evade immune surveillance (Marusina, K. and
Manaco, J. J. (1996) Curr. Opin. Hematol. 3:19-26).
ABC Transporters
[0019] The ATP-binding cassette (ABC) transporters, also called the
"traffic ATPases", comprise a superfamily of membrane proteins that
mediate transport and channel functions in prokaryotes and
eukaryotes (Higgins, C. F. (1992) Annu. Rev. Cell Biol. 8:67-113).
ABC proteins share a similar overall structure and significant
sequence homology. All ABC proteins contain a conserved domain of
approximately two hundred amino acid residues which includes one or
more nucleotide binding domains. Mutations in ABC transporter genes
are associated with various disorders, such as hyperbilirubinemia
II/Dubin-Johnson syndrome, recessive Stargardt's disease, X-linked
adrenoluekodystrophy, multidrug resistance, celiac disease, and
cystic fibrosis.
Membrane Proteins Associated with Intercellular Communication
[0020] Intercellular communication is essential for the development
and survival of multicellular organisms. Cells communicate with one
another through the secretion and uptake of protein signaling
molecules. The uptake of proteins into the cell is achieved by
endocytosis, in which the interaction of signaling molecules with
the plasma membrane surface, often via binding to specific
receptors, results in the formation of plasma membrane-derived
vesicles that enclose and transport the molecules into the cytosol.
The to secretion of proteins from the cell is achieved by
exocytosis, in which molecules inside of the cell are packaged into
membrane-bound transport vesicles derived from the trans-Golgi
network. These vesicles fuse with the plasma membrane and release
their contents into the surrounding extracellular space.
Endocytosis and exocytosis result in the removal and addition of
plasma membrane components and the recycling of these components is
essential to maintain the integrity, identity, and functionality of
both the plasma membrane and internal membrane-bound
compartments.
[0021] Lysosomes are the site of degradation of intracellular
material during autophagy and of extracellular molecules following
endocytosis. Lysosomal enzymes are packaged into vesicles which bud
from the trans-Golgi network. These vesicles fuse with endosomes to
form the mature lysosome in which hydrolytic digestion of
endocytosed material occurs. Lysosomes can fuse with autophagosomes
to form a unique compartment in which the degradation of organelles
and other intracellular components occurs. Protein sorting by
transport vesicles, such as the endosome, has important
consequences for a variety of physiological processes including
cell surface growth, the biogenesis of distinct intracellular
organelles, endocytosis, and the controlled secretion of hormones
and neurotransmitters (Rothman, J. E. and Wieland, F. T. (1996)
Science 272:227-234). In particular, neurodegenerative disorders
and other neuronal pathologies are associated with biochemical
flaws during endosomal protein sorting or endosomal biogenesis
(Mayer R. J. et al. (1996) Adv. Exp. Med. Biol. 389:261-269).
[0022] Peroxisomes are organelles independent from the secretory
pathway. They are the site of many peroxide-generating oxidative
reactions in the cell. Peroxisomes are unique among eukaryotic
organelles in that their size, number, and enzyme content vary
depending upon organism, cell type, and metabolic needs. The
majority of peroxisome-associated proteins are membrane-bound or
are found proximal to the cytosolic or the lumenal side of the
peroxisome membrane (Waterham, H. R. and Cregg, J. M. (1997)
BioEssays 19:57-66).
[0023] Genetic defects in peroxisome proteins which result in
peroxisomal deficiencies have been linked to a number of human
pathologies, including Zellweger syndrome, rhizomelic
chonrodysplasia punctata, X-linked adrenoleukodystrophy, acyl-CoA
oxidase deficiency, bifunctional enzyme deficiency, classical
Refsum's disease, DHAP alkyl transferase deficiency, and
acatalasemia (Moser, H. W. and Moser, A. B. (1996) Ann. NY Acad.
Sci. 804:427-441). In addition, Gartner, J. et al. (1991; Pediatr.
Res. 29:141-146) found a 22 kDa integral membrane protein
associated with lower density peroxisome-like subcellular fractions
in patients with Zellweger syndrome.
[0024] Normal embryonic development and control of germ cell
maturation is modulated by a number of secretory proteins which
interact with their respective membrane-bound receptors. Cell fate
during embryonic development is determined by members of the
activin/TGF-.beta. superfamily, cadherins, IGF-2, and other
morphogens. In addition, proliferation, maturation, and
redifferentiation of germ cell and reproductive tissues are
regulated, for example, by IGF-2, inhibins, activins, and
follistatins (Petraglia, F. (1997) Placenta 18:3-8; Mather, J. P.
et al. (1997) Proc. Soc. Exp. Biol. Med. 215:209-222).
Endoplasmic Reticulum Membrane Proteins
[0025] The normal functioning of the eukaryotic cell requires that
all newly synthesized proteins be correctly folded, modified, and
delivered to specific intra- and extracellular sites. Newly
synthesized membrane and secretory proteins enter a cellular
sorting and distribution network during or immediately after
synthesis and are routed to specific locations inside and outside
of the cell. The initial compartment in this process is the
endoplasmic reticulum (ER) where proteins undergo modifications
such as glycosylation, disulfide bond formation, and assembly into
oligomers. The modified proteins are then transported through a
series of membrane-bound compartments which include the various
cisternae of the Golgi complex, where further carbohydrate
modifications occur. Transport between compartments occurs by means
of vesicles that bud and fuse in a manner specific to the type of
protein being transported. Once within the secretory pathway,
proteins do not have to cross a membrane to reach the cell
surface.
[0026] Although the majority of proteins processed through the ER
are transported out of the organelle, some are retained. The signal
for retention in the ER in mammalian cells consists of the
tetrapeptide sequence, KDEL, located at the carboxyl terminus of
proteins (Munro, S. (1986) Cell 46:291-300). Proteins containing
this sequence leave the ER but are quickly retrieved from the early
Golgi cisternae and returned to the ER, while proteins lacking this
signal continue through the secretory pathway.
[0027] Disruptions in the cellular secretory pathway have been
implicated in several human diseases. In familial
hypercholesterolemia the low density lipoprotein receptors remain
in the ER, rather than moving to the cell surface (Pathak, R. K.
(1988) J. Cell Biol. 106:1831-1841). Altered transport and
processing of the .beta.-amyloid precursor protein (PAPP) involves
the putative vesicle transport protein presenilin, and may play a
role in early-onset Alzheimer's disease (Levy-Lahad. E. et al.
(1995) Science 269:973-977). Changes in ER-derived calcium
homeostasis have been associated with diseases such as
cardiomyopathy, cardiac hypertrophy, myotonic dystrophy, Brody
disease, Smith-McCort dysplasia, and diabetes mellitus.
Mitochondrial Membrane Proteins
[0028] The mitochondrial electron transport (or respiratory) chain
is a series of three enzyme complexes in the mitochondrial membrane
that is responsible for the transport of electrons from NADH to
oxygen and the coupling of this oxidation to the synthesis of ATP
(oxidative phosphorylation). ATP then provides the primary source
of energy for driving the many energy-requiring reactions of a
cell.
[0029] Most of the protein components of the mitochondrial
respiratory chain are the products of nuclear encoded genes that
are imported into the mitochondria and the remainder are products
of mitochondrial genes. Defects and altered expression of enzymes
in the respiratory chain are associated with a variety of disease
conditions in man, including, for example, neurodegenerative
diseases, myopathies, and cancer.
Lymphocyte and Leukocyte Membrane Proteins
[0030] The B-cell response to antigens, which is modulated through
receptors, is an essential component of the normal immune system.
Mature B cells recognize foreign antigens through B cell receptors
(BCR) which are membrane-bound, specific antibodies that bind
foreign antigens. The antigen/receptor complex is internalized and
the antigen is proteolytically processed. To generate an efficient
response to complex antigens, the BCR, BCR-associated proteins, and
T cell response are all required. Proteolytic fragments of the
antigen are complexed with major histocompatability complex-II
(MHCII) molecules on the surface of the B cells where the complex
can be recognized by T cells. In contrast, macrophages and other
lymphoid cells present antigens in association with MHCI molecules
to T cells. T cells recognize and are activated by the MHCI-antigen
complex through interactions with the T cell receptor/CD3 complex,
a T cell-surface multimeric protein located in the plasma membrane.
T cells activated by antigen presentation secrete a variety of
lymphokines that induce B cell maturation and T cell proliferation
and activate macrophages, which kill target cells.
[0031] Leukocytes have a fundamental role in the inflammatory and
immune response and include monocytes/macrophages, mast cells,
polymorphonucleoleukocytes, natural killer cells, neutrophils,
eosinophils, basophils, and myeloid precursors. Leukocyte membrane
proteins include members of the CD antigens, N-CAM, I-CAM, human
leukocyte antigen (HLA) class I and HLA class II gene products,
immunoglobulins, immunoglobulin receptors, complement, complement
receptors, interferons, interferon receptors, interleukin
receptors, and chemokine receptors.
[0032] Abnormal lymphocyte and leukocyte activity has been
associated with acute disorders, such as AIDS, immune
hypersensitivity, leukemias, leukopenia, systemic lupus,
granulomatous disease, and eosinophilia.
Apoptosis-Associated Membrane Proteins
[0033] A variety of ligands, receptors, enzymes, tumor suppressors,
viral gene products, pharmacological agents, and inorganic ions
have important positive or negative roles in regulating and
implementing the apoptotic destruction of a cell. Although some
specific components of the apoptotic pathway have been identified
and characterized, many interactions between the proteins involved
are undefined, leaving major aspects of the pathway unknown.
[0034] A requirement for calcium in apoptosis was previously
suggested by studies showing the involvement of calcium levels in
DNA cleavage and Fas-mediated cell death (Hewish, D. R. and L. A.
Burgoyne (1973) Biochem. Biophys. Res. Comm. 52:504-510; Vignaux,
F. et al. (1995) J. Exp. Med. 181:781-786; Oshimi, Y. and S.
Miyazaki (1995) J. Immunol. 154:599-609). Other studies show that
intracellular calcium concentrations increase when apoptosis is
triggered in thymocytes by either T cell receptor cross-linking or
by glucocorticoids and cell death can be prevented by blocking this
increase (McConkey, D. J. et al. (1989) J. Immunol. 143:1801-1806;
McConkey, D. J. et al. (1989) Arch. Biochem. Biophys. 269:365-370).
Therefore, membrane proteins such as calcium channels are important
for the apopoptic response.
Tumorgenesis
[0035] Tumorgenesis is associated with the activation of oncogenes
which are derived from normal cellular genes. These oncogenes
encode oncoproteins which are capable of converting normal cells
into malignant cells. Some oncoproteins are mutant isoforms of the
normal protein and other oncoproteins are abnormally expressed with
respect to location or level of expression. The latter category of
oncoprotein causes cancer by altering transcriptional control of
cell proliferation. Five classes of oncoproteins are known to
affect the cell cycle controls. These classes include growth
factors, growth factor receptors, intracellular signal transducers,
nuclear transcription factors, and cell-cycle control proteins.
These proteins include those which are modified by glycosylation,
phosphorylation, glycosaminoglycan attachment, sulphation, and
lipidation.
[0036] Modulation of factors which act in the coordination of the
human cell division cycle may provide an important means to reduce
tumorgenesis. An example of the metastasis-associated proteins is
the lysosomal membrane glycoprotein P2B/LAMP-1 which is also
expressed in normal tissues. (Heffernan, M. et al. (1989) Cancer
Res. 49:6077-6084.) In addition, mammalian proteins homologous to
the plant pathogenesis-related proteins have been identified in
hyperplastic glioma. (Murphy, E. V. et al. (1995) Gene
159:131-135.)
[0037] The discovery of new human transmembrane proteins and the
polynucleotides encoding them satisfies a need in the art by
providing new compositions which are useful in the diagnosis,
prevention, and treatment of immune, reproductive, smooth muscle,
neurological, gastrointestinal, developmental, and cell
proliferative disorders.
SUMMARY OF THE INVENTION
[0038] The invention features substantially purified polypeptides,
human transmembrane proteins, referred to collectively as "HTMPN"
and individually as "HTMPN-1", "HTMPN-2", "HTMPN-3", "HTMPN-4",
"HTMPN-5", "HTMPN-6", "HTMPN-7", "HTMPN-8", "HTMPN-9", "HTMPN-10",
"HTMPN-11", "HTMPN-12", "HTMPN-13", "HTMPN-14", "HTMPN-15",
"HTMPN-16", "HTMPN-17", "HTMPN-18", "HTMPN-19", "HTMPN-20",
"HTMPN-21", "HTMPN-22", "HTMPN-23", "HTMPN-24", "HTMPN-25",
"HTMPN-26", "HTMPN-27", "HTMPN-28", "HTMPN-29", "HTMPN-30",
"HTMPN-31", "HTMPN-32", "HTMPN-33", "HTMPN-34", "HTMPN-35",
"HTMPN-36", "HTMPN-37", "HTMPN-38", "HTMPN-39", "HTMPN-40",
"HTMPN-41", "HTMPN-42", "HTMPN-43", "HTMPN-44", "HTMPN-45",
"HTMPN-46", "HTMPN-47", "HTMPN-48", "HTMPN-49", "HTMPN-50",
"HTMPN-51", "HTMPN-52", "HTMPN-53", "HTMPN-54", "HTMPN-55",
"HTMPN-56", "HTMPN-57", "HTMPN-58", "HTMPN-59", "HTMPN-60",
"HTMPN-61", "HTMPN-62", "HTMPN-63", "HTMPN-64", "HTMPN-65",
"HTMPN-66", "HTMPN-67", "HTMPN-68", "HTMPN-69", "HTMPN-70",
"HTMPN-71", "HTMPN-72", "HTMPN-73", "HTMPN-74", "HTMPN-75",
"HTMPN-76", "HTMPN-77", "HTMPN-78", and "HTMPN-79". In one aspect,
the invention provides a substantially purified polypeptide
comprising an amino acid sequence selected from the group
consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4,
SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9,
SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID
NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ
ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23,
SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID
NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ
ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37,
SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID
NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ
ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51,
SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID
NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ
ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65,
SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID
NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:73, SEQ ID NO:74, SEQ
ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, and SEQ ID
NO:79 (SEQ ID NO:1-79), and fragments thereof.
[0039] The invention further provides a substantially purified
variant having at least 90% amino acid identity to at least one of
the amino acid sequences selected from the group consisting of SEQ
ID NO:1-79, and fragments thereof. The invention also provides an
isolated and purified polynucleotide encoding the polypeptide
comprising an amino acid sequence selected from the group
consisting of SEQ ID NO:1-79, and fragments thereof. The invention
also includes an isolated and purified polynucleotide variant
having at least 90% polynucleotide sequence identity to the
polynucleotide encoding the polypeptide comprising an amino acid
sequence selected from the group consisting of SEQ ID NO:1-79, and
fragments thereof.
[0040] Additionally, the invention provides an isolated and
purified polynucleotide which hybridizes under stringent conditions
to the polynucleotide encoding the polypeptide comprising an amino
acid sequence selected from the group consisting of SEQ ID NO:1-79,
and fragments thereof. The invention also provides an isolated and
purified polynucleotide having a sequence which is complementary to
the polynucleotide encoding the polypeptide comprising the amino
acid sequence selected from the group consisting of SEQ ID NO:1-79,
and fragments thereof.
[0041] The invention also provides an isolated and purified
polynucleotide comprising a polynucleotide sequence selected from
the group consisting of SEQ ID NO:80, SEQ ID NO:81, SEQ ID NO:82,
SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID
NO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:90, SEQ ID NO:91, SEQ
ID NO:92, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:95, SEQ ID NO:96,
SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:100, SEQ ID
NO:101, SEQ ID NO:102, SEQ ID NO:103, SEQ ID NO:104, SEQ ID NO:105,
SEQ ID NO:106, SEQ ID NO:107, SEQ ID NO:108, SEQ ID NO:109, SEQ ID
NO:110, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ ID NO:114,
SEQ ID NO:115, SEQ ID NO:116, SEQ ID NO:117, SEQ ID NO:118, SEQ ID
NO:119, SEQ ID NO:120, SEQ ID NO:121, SEQ ID NO:122, SEQ ID NO:123,
SEQ ID NO:124, SEQ ID NO:125, SEQ ID NO:126, SEQ ID NO:127, SEQ ID
NO:128, SEQ ID NO:129, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132,
SEQ ID NO:133, SEQ ID NO:134, SEQ ID NO:135, SEQ ID NO:136, SEQ ID
NO:137, SEQ ID NO:138, SEQ ID NO:139, SEQ ID NO:140, SEQ ID NO:141,
SEQ ID NO:142, SEQ ID NO:143, SEQ ID NO:144, SEQ ID NO:145, SEQ ID
NO:146, SEQ ID NO:147, SEQ ID NO:148, SEQ ID NO:149, SEQ ID NO:150,
SEQ ID NO:151, SEQ ID NO:152, SEQ ID NO:153, SEQ ID NO:154, SEQ ID
NO:155, SEQ ID NO:156, SEQ ID NO:157, and SEQ ID NO:158 (SEQ ID
NO:80-158), and fragments thereof. The invention further provides
an isolated and purified polynucleotide variant having at least 90%
polynucleotide sequence identity to the polynucleotide sequence
selected from the group consisting of SEQ ID NO:80-158, and
fragments thereof. The invention also provides an isolated and
purified polynucleotide having a sequence which is complementary to
the polynucleotide comprising a polynucleotide sequence selected
from the group consisting of SEQ ID NO:80-158, and fragments
thereof.
[0042] The invention also provides a method for detecting a
polynucleotide in a sample containing nucleic acids, the method
comprising the steps of (a) hybridizing the complement of the
polynucleotide sequence to at least one of the polynucleotides of
the sample, thereby forming a hybridization complex; and (b)
detecting the hybridization complex, wherein the presence of the
hybridization complex correlates with the presence of a
polynucleotide in the sample. In one aspect, the method further
comprises amplifying the polynucleotide prior to hybridization.
[0043] The invention further provides an expression vector
containing at least a fragment of the polynucleotide encoding the
polypeptide comprising an amino acid sequence selected from the
group consisting of SEQ ID NO:1-79, and fragments thereof. In
another aspect, the expression vector is contained within a host
cell.
[0044] The invention also provides a method for producing a
polypeptide, the method comprising the steps of: (a) culturing the
host cell containing an expression vector containing at least a
fragment of a polynucleotide under conditions suitable for the
expression of the polypeptide; and (b) recovering the polypeptide
from the host cell culture.
[0045] The invention also provides a pharmaceutical composition
comprising a substantially purified polypeptide having the amino
acid sequence selected from the group consisting of SEQ ID NO:1-79,
and fragments thereof, in conjunction with a suitable
pharmaceutical carrier.
[0046] The invention further includes a purified antibody which
binds to a polypeptide selected from the group consisting of SEQ ID
NO:1-79, and fragments thereof. The invention also provides a
purified agonist and a purified antagonist to the polypeptide.
[0047] The invention also provides a method for treating or
preventing a disorder associated with decreased expression or
activity of HTMPN, the method comprising administering to a subject
in need of such treatment an effective amount of a pharmaceutical
composition comprising a substantially purified polypeptide having
the amino acid sequence selected from the group consisting of SEQ
ID NO:1-79, and fragments thereof, in conjunction with a suitable
pharmaceutical carrier.
[0048] The invention also provides a method for treating or
preventing a disorder associated with increased expression or
activity of HTMPN, the method comprising administering to a subject
in need of such treatment an effective amount of an antagonist of a
polypeptide having an amino acid sequence selected from the group
consisting of SEQ ID NO:1-79, and fragments thereof.
BRIEF DESCRIPTION OF THE TABLES
[0049] Table 1 shows nucleotide and polypeptide sequence
identification numbers (SEQ ID NOs), clone identification numbers
(clone ID), cDNA libraries, and cDNA fragments used to assemble
full-length sequences encoding HTMPN.
[0050] Table 2 shows features of each polypeptide sequence
including predicted transmembrane sequences, potential motifs,
homologous sequences, and methods and algorithms used for
identification of HTMPN.
[0051] Table 3 shows the tissue-specific expression patterns of
each nucleic acid sequence as determined by northern analysis,
diseases, disorders, or conditions associated with these tissues,
and the vector into which each cDNA was cloned.
[0052] Table 4 describes the tissues used to construct the cDNA
libraries from which Incyte cDNA clones encoding HTMPN were
isolated.
[0053] Table 5 shows the programs, their descriptions, references,
and threshold parameters used to analyze HTMPN.
DESCRIPTION OF THE INVENTION
[0054] Before the present proteins, nucleotide sequences, and
methods are described, it is understood that this invention is not
limited to the particular machines, materials and methods
described, as these may vary. It is also to be understood that the
terminology used herein is for the purpose of describing particular
embodiments only, and is not intended to limit the scope of the
present invention which will be limited only by the appended
claims.
[0055] It must be noted that as used herein and in the appended
claims, the singular forms "a," "an," and "the" include plural
reference unless the context clearly dictates otherwise. Thus, for
example, a reference to "a host cell" includes a plurality of such
host cells, and a reference to "an antibody" is a reference to one
or more antibodies and equivalents thereof known to those skilled
in the art, and so forth.
[0056] Unless defined otherwise, all technical and scientific terms
used herein have the same meanings as commonly understood by one of
ordinary skill in the art to which this invention belongs. Although
any machines, materials, and methods similar or equivalent to those
described herein can be used to practice or test the present
invention, the preferred machines, materials and methods are now
described. All publications mentioned herein are cited for the
purpose of describing and disclosing the cell lines, protocols,
reagents and vectors which are reported in the publications and
which might be used in connection with the invention. Nothing
herein is to be construed as an admission that the invention is not
entitled to antedate such disclosure by virtue of prior
invention.
DEFINITIONS
[0057] "HTMPN" refers to the amino acid sequences of substantially
purified HTMPN obtained from any species, particularly a mammalian
species, including bovine, ovine, porcine, murine, equine, and
preferably the human species, from any source, whether natural,
synthetic, semi-synthetic, or recombinant.
[0058] The term "agonist" refers to a molecule which, when bound to
HTMPN, increases or prolongs the duration of the effect of HTMPN.
Agonists may include proteins, nucleic acids, carbohydrates, or any
other molecules which bind to and modulate the effect of HTMPN.
[0059] An "allelic variant" is an alternative form of the gene
encoding HTMPN. Allelic variants may result from at least one
mutation in the nucleic acid sequence and may result in altered
mRNAs or in polypeptides whose structure or function may or may not
be altered. Any given natural or recombinant gene may have none,
one, or many allelic forms. Common mutational changes which give
rise to allelic variants are generally ascribed to natural
deletions, additions, or substitutions of nucleotides. Each of
these types of changes may occur alone, or in combination with the
others, one or more times in a given sequence.
[0060] "Altered" nucleic acid sequences encoding HTMPN include
those sequences with deletions, insertions, or substitutions of
different nucleotides, resulting in a polynucleotide the same as
HTMPN or a polypeptide with at least one functional characteristic
of HTMPN. Included within this definition are polymorphisms which
may or may not be readily detectable using a particular
oligonucleotide probe of the polynucleotide encoding HTMPN, and
improper or unexpected hybridization to allelic variants, with a
locus other than the normal chromosomal locus for the
polynucleotide sequence encoding HTMPN. The encoded protein may
also be "altered," and may contain deletions, insertions, or
substitutions of amino acid residues which produce a silent change
and result in a functionally equivalent HTMPN. Deliberate amino
acid substitutions may be made on the basis of similarity in
polarity, charge, solubility, hydrophobicity, hydrophilicity,
and/or the amphipathic nature of the residues, as long as the
biological or immunological activity of HTMPN is retained. For
example, negatively charged amino acids may include aspartic acid
and glutamic acid, positively charged amino acids may include
lysine and arginine, and amino acids with uncharged polar head
groups having similar hydrophilicity values may include leucine,
isoleucine, and valine; glycine and alanine; asparagine and
glutamine; serine and threonine; and phenylalanine and
tyrosine.
[0061] The terms "amino acid" or "amino acid sequence" refer to an
oligopeptide, peptide, polypeptide, or protein sequence, or a
fragment of any of these, and to naturally occurring or synthetic
molecules. In this context, "fragments," "immunogenic fragments,"
or "antigenic fragments" refer to fragments of HTMPN which are
preferably at least 5 to about 15 amino acids in length, most
preferably at least 14 amino acids, and which retain some
biological activity or immunological activity of HTMPN. Where
"amino acid sequence" is recited to refer to an amino acid sequence
of a naturally occurring protein molecule, "amino acid sequence"
and like terms are not meant to limit the amino acid sequence to
the complete native amino acid sequence associated with the recited
protein molecule.
[0062] "Amplification" relates to the production of additional
copies of a nucleic acid sequence. Amplification is generally
carried out using polymerase chain reaction (PCR) technologies well
known in the art.
[0063] The term "antagonist" refers to a molecule which, when bound
to HTMPN, decreases the amount or the duration of the effect of the
biological or immunological activity of HTMPN. Antagonists may
include proteins, nucleic acids, carbohydrates, antibodies, or any
other molecules which decrease the effect of HTMPN.
[0064] The term "antibody" refers to intact molecules as well as to
fragments thereof, such as Fab, F(ab').sub.2, and Fv fragments,
which are capable of binding the epitopic determinant. Antibodies
that bind HTMPN polypeptides can be prepared using intact
polypeptides or using fragments containing small peptides of
interest as the immunizing antigen. The polypeptide or oligopeptide
used to immunize an animal (e.g., a mouse, a rat, or a rabbit) can
be derived from the translation of RNA, or synthesized chemically,
and can be conjugated to a carrier protein if desired. Commonly
used carriers that are chemically coupled to peptides include
bovine serum albumin, thyroglobulin, and keyhole limpet hemocyanin
(KLH). The coupled peptide is then used to immunize the animal.
[0065] The term "antigenic determinant" refers to that fragment of
a molecule (i.e., an epitope) that makes contact with a particular
antibody. When a protein or a fragment of a protein is used to
immunize a host animal, numerous regions of the protein may induce
the production of antibodies which bind specifically to antigenic
determinants (given regions or three-dimensional structures on the
protein). An antigenic determinant may compete with the intact
antigen (i.e., the immunogen used to elicit the immune response)
for binding to an antibody.
[0066] The term "antisense" refers to any composition containing a
nucleic acid sequence which is complementary to the "sense" strand
of a specific nucleic acid sequence. Antisense molecules may be
produced by any method including synthesis or transcription. Once
introduced into a cell, the complementary nucleotides combine with
natural sequences produced by the cell to form duplexes and to
block either transcription or translation. The designation
"negative" can refer to the antisense strand, and the designation
"positive" can refer to the sense strand.
[0067] The term "biologically active," refers to a protein having
structural, regulatory, or biochemical functions of a naturally
occurring molecule. Likewise, "immunologically active" refers to
the capability of the natural, recombinant, or synthetic HTMPN, or
of any oligopeptide thereof, to induce a specific immune response
in appropriate animals or cells and to bind with specific
antibodies.
[0068] The terms "complementary" or "complementarity" refer to the
natural binding of polynucleotides by base pairing. For example,
the sequence "5' A-G-T 3'" bonds to the complementary sequence "3'
T-C-A 5'." Complementarity between two single-stranded molecules
may be "partial," such that only some of the nucleic acids bind, or
it may be "complete," such that total complementarity exists
between the single stranded molecules. The degree of
complementarity between nucleic acid strands has significant
effects on the efficiency and strength of the hybridization between
the nucleic acid strands. This is of particular importance in
amplification reactions, which depend upon binding between nucleic
acids strands, and in the design and use of peptide nucleic acid
(PNA) molecules.
[0069] A "composition comprising a given polynucleotide sequence"
or a "composition comprising a given amino acid sequence" refer
broadly to any composition containing the given polynucleotide or
amino acid sequence. The composition may comprise a dry formulation
or an aqueous solution. Compositions comprising polynucleotide
sequences encoding HTMPN or fragments of HTMPN may be employed as
hybridization probes. The probes may be stored in freeze-dried form
and may be associated with a stabilizing agent such as a
carbohydrate. In hybridizations, the probe may be deployed in an
aqueous solution containing salts (e.g., NaCl), detergents (e.g.,
sodium dodecyl sulfate; SDS), and other components (e.g.,
Denhardt's solution, dry milk, salmon sperm DNA, etc.).
[0070] "Consensus sequence" refers to a nucleic acid sequence which
has been resequenced to resolve uncalled bases, extended using
XL-PCR kit (Perkin-Elmer, Norwalk Conn.) in the 5' and/or the 3'
direction, and resequenced, or which has been assembled from the
overlapping sequences of more than one Incyte Clone using a
computer program for fragment assembly, such as the GELVIEW
Fragment Assembly system (GCG, Madison Wis.). Some sequences have
been both extended and assembled to produce the consensus
sequence.
[0071] The term "correlates with expression of a polynucleotide"
indicates that the detection of the presence of nucleic acids, the
same or related to a nucleic acid sequence encoding HTMPN, by
northern analysis is indicative of the presence of nucleic acids
encoding HTMPN in a sample, and thereby correlates with expression
of the transcript from the polynucleotide encoding HTMPN.
[0072] A "deletion" refers to a change in the amino acid or
nucleotide sequence that results in the absence of one or more
amino acid residues or nucleotides.
[0073] The term "derivative" refers to the chemical modification of
a polypeptide sequence, or a polynucleotide sequence. Chemical
modifications of a polynucleotide sequence can include, for
example, replacement of hydrogen by an alkyl, acyl, or amino group.
A derivative polynucleotide encodes a polypeptide which retains at
least one biological or immunological function of the natural
molecule. A derivative polypeptide is one modified by
glycosylation, pegylation, or any similar process that retains at
least one biological or immunological function of the polypeptide
from which it was derived.
[0074] The term "similarity" refers to a degree of complementarity.
There may be partial similarity or complete similarity. The word
"identity" may substitute for the word "similarity." A partially
complementary sequence that at least partially inhibits an
identical sequence from hybridizing to a target nucleic acid is
referred to as "substantially similar." The inhibition of
hybridization of the completely complementary sequence to the
target sequence may be examined using a hybridization assay
(Southern or northern blot, solution hybridization, and the like)
under conditions of reduced stringency. A substantially similar
sequence or hybridization probe will compete for and inhibit the
binding of a completely similar (identical) sequence to the target
sequence under conditions of reduced stringency. This is not to say
that conditions of reduced stringency are such that non-specific
binding is permitted, as reduced stringency conditions require that
the binding of two sequences to one another be a specific (i.e., a
selective) interaction. The absence of non-specific binding may be
tested by the use of a second target sequence which lacks even a
partial degree of complementarity (e.g., less than about 30%
similarity or identity). In the absence of non-specific binding,
the substantially similar sequence or probe will not hybridize to
the second non-complementary target sequence.
[0075] The phrases "percent identity" or "% identity" refer to the
percentage of sequence similarity found in a comparison of two or
more amino acid or nucleic acid sequences. Percent identity can be
determined electronically, e.g., by using the MEGALIGN program
(DNASTAR, Madison Wis.) which creates alignments between two or
more sequences according to methods selected by the user, e.g., the
clustal method. (See, e.g., Higgins, D. G. and P. M. Sharp (1988)
Gene 73:237-244.) The clustal algorithm groups sequences into
clusters by examining the distances between all pairs. The clusters
are aligned pairwise and then in groups. The percentage similarity
between two amino acid sequences, e.g., sequence A and sequence B,
is calculated by dividing the length of sequence A, minus the
number of gap residues in sequence A, minus the number of gap
residues in sequence B, into the sum of the residue matches between
sequence A and sequence B, times one hundred. Gaps of low or of no
similarity between the two amino acid sequences are not included in
determining percentage similarity. Percent identity between nucleic
acid sequences can also be counted or calculated by other methods
known in the art, e.g., the Jotun Hein method. (See, e.g., Hein, J.
(1990) Methods Enzymol. 183:626-645.) Identity between sequences
can also be determined by other methods known in the art, e.g., by
varying hybridization conditions.
[0076] "Human artificial chromosomes" (HACs) are linear
microchromosomes which may contain DNA sequences of about 6 kb to
10 Mb in size, and which contain all of the elements required for
stable mitotic chromosome segregation and maintenance.
[0077] The term "humanized antibody" refers to antibody molecules
in which the amino acid sequence in the non-antigen binding regions
has been altered so that the antibody more closely resembles a
human antibody, and still retains its original binding ability.
[0078] "Hybridization" refers to any process by which a strand of
nucleic acid binds with a complementary strand through base
pairing.
[0079] The term "hybridization complex" refers to a complex formed
between two nucleic acid sequences by virtue of the formation of
hydrogen bonds between complementary bases. A hybridization complex
may be formed in solution (e.g., C.sub.0t or R.sub.0t analysis) or
formed between one nucleic acid sequence present in solution and
another nucleic acid sequence immobilized on a solid support (e.g.,
paper, membranes, filters, chips, pins or glass slides, or any
other appropriate substrate to which cells or their nucleic acids
have been fixed).
[0080] The words "insertion" or "addition" refer to changes in an
amino acid or nucleotide sequence resulting in the addition of one
or more amino acid residues or nucleotides, respectively, to the
sequence found in the naturally occurring molecule.
[0081] "Immune response" can refer to conditions associated with
inflammation, trauma, immune disorders, or infectious or genetic
disease, etc. These conditions can be characterized by expression
of various factors, e.g., cytokines, chemokines, and other
signaling molecules, which may affect cellular and systemic defense
systems.
[0082] The term "microarray" refers to an arrangement of distinct
polynucleotides on a substrate.
[0083] The terms "element" or "array element" in a microarray
context, refer to hybridizable polynucleotides arranged on the
surface of a substrate.
[0084] The term "modulate" refers to a change in the activity of
HTMPN. For example, modulation may cause an increase or a decrease
in protein activity, binding characteristics, or any other
biological, functional, or immunological properties of HTMPN.
[0085] The phrases "nucleic acid" or "nucleic acid sequence" refer
to a nucleotide, oligonucleotide, polynucleotide, or any fragment
thereof. These phrases also refer to DNA or RNA of genomic or
synthetic origin which may be single-stranded or double-stranded
and may represent the sense or the antisense strand, to peptide
nucleic acid (PNA), or to any DNA-like or RNA-like material. In
this context, "fragments" refers to those nucleic acid sequences
which, when translated, would produce polypeptides retaining some
functional characteristic, e.g., antigenicity, or structural domain
characteristic, e.g., ATP-binding site, of the full-length
polypeptide.
[0086] The terms "operably associated" or "operably linked" refer
to functionally related nucleic acid sequences. A promoter is
operably associated or operably linked with a coding sequence if
the promoter controls the translation of the encoded polypeptide.
While operably associated or operably linked nucleic acid sequences
can be contiguous and in the same reading frame, certain genetic
elements, e.g., repressor genes, are not contiguously linked to the
sequence encoding the polypeptide but still bind to operator
sequences that control expression of the polypeptide.
[0087] The term "oligonucleotide" refers to a nucleic acid sequence
of at least about 6 nucleotides to 60 nucleotides, preferably about
15 to 30 nucleotides, and most preferably about 20 to 25
nucleotides, which can be used in PCR amplification or in a
hybridization assay or microarray. "Oligonucleotide" is
substantially equivalent to the terms "amplimer," "primer."
"oligomer," and "probe," as these terms are commonly defined in the
art.
[0088] "Peptide nucleic acid" (PNA) refers to an antisense molecule
or anti-gene agent which comprises an oligonucleotide of at least
about 5 nucleotides in length linked to a peptide backbone of amino
acid residues ending in lysine. The terminal lysine confers
solubility to the composition. PNAs preferentially bind
complementary single stranded DNA or RNA and stop transcript
elongation, and may be pegylated to extend their lifespan in the
cell.
[0089] The term "sample" is used in its broadest sense. A sample
suspected of containing nucleic acids encoding HTMPN, or fragments
thereof, or HTMPN itself, may comprise a bodily fluid; an extract
from a cell, chromosome, organelle, or membrane isolated from a
cell; a cell; genomic DNA, RNA, or cDNA, in solution or bound to a
substrate; a tissue; a tissue print; etc.
[0090] The terms "specific binding" or "specifically binding" refer
to that interaction between a protein or peptide and an agonist, an
antibody, or an antagonist. The interaction is dependent upon the
presence of a particular structure of the protein, e.g., the
antigenic determinant or epitope, recognized by the binding
molecule. For example, if an antibody is specific for epitope "A,"
the presence of a polypeptide containing the epitope A, or the
presence of free unlabeled A, in a reaction containing free labeled
A and the antibody will reduce the amount of labeled A that binds
to the antibody.
[0091] The term "stringent conditions" refers to conditions which
permit hybridization between polynucleotides and the claimed
polynucleotides. Stringent conditions can be defined by salt
concentration, the concentration of organic solvent, e.g.,
formamide, temperature, and other conditions well known in the art.
In particular, stringency can be increased by reducing the
concentration of salt, increasing the concentration of formamide,
or raising the hybridization temperature.
[0092] The term "substantially purified" refers to nucleic acid or
amino acid sequences that are removed from their natural
environment and are isolated or separated, and are at least about
60% free, preferably about 75% free, and most preferably about 90%
free from other components with which they are naturally
associated.
[0093] A "substitution" refers to the replacement of one or more
amino acids or nucleotides by different amino acids or nucleotides,
respectively.
[0094] "Substrate" refers to any suitable rigid or semi-rigid
support including membranes, filters, chips, slides, wafers,
fibers, magnetic or nonmagnetic beads, gels, tubing, plates,
polymers, microparticles and capillaries. The substrate can have a
variety of surface forms, such as wells, trenches, pins, channels
and pores, to which polynucleotides or polypeptides are bound.
[0095] "Transformation" describes a process by which exogenous DNA
enters and changes a recipient cell. Transformation may occur under
natural or artificial conditions according to various methods well
known in the art, and may rely on any known method for the
insertion of foreign nucleic acid sequences into a prokaryotic or
eukaryotic host cell. The method for transformation is selected
based on the type of host cell being transformed and may include,
but is not limited to, viral infection, electroporation, heat
shock, lipofection, and particle bombardment. The term
"transformed" cells includes stably transformed cells in which the
inserted DNA is capable of replication either as an autonomously
replicating plasmid or as part of the host chromosome, as well as
transiently transformed cells which express the inserted DNA or RNA
for limited periods of time.
[0096] A "variant" of HTMPN polypeptides refers to an amino acid
sequence that is altered by one or more amino acid residues. The
variant may have "conservative" changes, wherein a substituted
amino acid has similar structural or chemical properties (e.g.,
replacement of leucine with isoleucine). More rarely, a variant may
have "nonconservative" changes (e.g., replacement of glycine with
tryptophan). Analogous minor variations may also include amino acid
deletions or insertions, or both. Guidance in determining which
amino acid residues may be substituted, inserted, or deleted
without abolishing biological or immunological activity may be
found using computer programs well known in the art, for example,
LASERGENE software (DNASTAR).
[0097] The term "variant," when used in the context of a
polynucleotide sequence, may encompass a polynucleotide sequence
related to HTMPN. This definition may also include, for example,
"allelic" (as defined above), "splice," "species," or "polymorphic"
variants. A splice variant may have significant identity to a
reference molecule, but will generally have a greater or lesser
number of polynucleotides due to alternate splicing of exons during
mRNA processing. The corresponding polypeptide may possess
additional functional domains or an absence of domains. Species
variants are polynucleotide sequences that vary from one species to
another. The resulting polypeptides generally will have significant
amino acid identity relative to each other. A polymorphic variant
is a variation in the polynucleotide sequence of a particular gene
between individuals of a given species. Polymorphic variants also
may encompass "single nucleotide polymorphisms" (SNPs) in which the
polynucleotide sequence varies by one base. The presence of SNPs
may be indicative of, for example, a certain population, a disease
state, or a propensity for a disease state.
The Invention
[0098] The invention is based on the discovery of new human
transmembrane proteins (HTMPN), the polynucleotides encoding HTMPN,
and the use of these compositions for the diagnosis, treatment, or
prevention of immune, reproductive, smooth muscle, neurological,
gastrointestinal, developmental, and cell proliferative
disorders.
[0099] Table 1 lists the Incyte Clones used to derive full length
nucleotide sequences encoding HTMPN. Columns 1 and 2 show the
sequence identification numbers (SEQ ID NOs) of the amino acid and
nucleic acid sequences, respectively. Column 3 shows the Clone ID
of the Incyte Clone in which nucleic acids encoding each HTMPN were
identified, and column 4, the cDNA libraries from which these
clones were isolated. Column 5 shows Incyte clones, their
corresponding cDNA libraries, and shotgun sequences. The clones and
shotgun sequences are part of the consensus nucleotide sequence of
each HTMPN and are useful as fragments in hybridization
technologies.
[0100] The columns of Table 2 show various properties of the
polypeptides of the invention: column 1 references the SEQ ID NO;
column 2 shows the number of amino acid residues in each
polypeptide; column 3, potential phosphorylation sites; column 4,
potential glycosylation sites; column 5, the amino acid residues
comprising signature sequences and motifs; column 6, the identity
of each protein; and column 7, analytical methods used to identify
each protein through sequence homology and protein motifs. Hidden
Markov Model analysis indicates the presence of one or more
potential transmembrane motifs in each of SEQ ID NO:63, SEQ ID
NO:64, SEQ ID NO: 66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ
ID NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:73, SEQ ID NO:74,
SEQ ID NO: 75, SEQ ID NO:76, SEQ ID NO:77, and SEQ ID NO: 79; as
well as the presence of one or more potential signal peptide motifs
in each of SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:66, SEQ ID NO:67,
SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:75, SEQ ID NO:77, and SEQ ID
NO:79.
[0101] Motifs analysis indicates the presence of a potential
ATP/GTP binding site in SEQ ID NO:68, a potential calcium-binding
site also in SEQ ID NO:68, a potential leucine zipper gene
regulatory motif in each of SEQ ID NO:68 and SEQ ID NO:73; and a
potential microbody (single-membraned organelle) targeting signal
site in SEQ ID NO:78. BLOCKS analysis indicates the presence of two
potential PMP-22 integral membrane glycoprotein motifs and a
trehalase motif, all in SEQ ID NO:77, as well as a potential
protein-splicing motif in SEQ ID NO:66. PRINTS analysis indicates
the presence of a potential G-protein coupled receptor motif in SEQ
ID NO:79.
[0102] The columns of Table 3 show the tissue-specificity and
diseases, disorders, or conditions associated with nucleotide
sequences encoding HTMPN. The first column of Table 3 lists the
nucleotide sequence identifiers. The second column lists tissue
categories which express HTMPN as a fraction of total tissue
categories expressing HTMPN. The third column lists the diseases,
disorders, or conditions associated with those tissues expressing
HTMPN. The fourth column lists the vectors used to subclone the
cDNA library. Of particular note is the expression of HTMPN in
tissue involved in inflammation and the immune response and with
cell proliferative conditions including cancer, and in
reproductive, gastrointestinal, fetal, smooth muscle,
cardiovascular, urologic, endocrine, developmental, and nervous
tissue.
[0103] The following fragments of the nucleotide sequences encoding
HTMPN are useful in hybridization or amplification technologies to
identify SEQ ID NO:121-158 and to distinguish between SEQ ID
NO:121-158 and related polynucleotide sequences. The useful
fragments are the fragment of SEQ ID NO:121 from about nucleotide
151 to about nucleotide 189; the fragment of SEQ ID NO:122 from
about nucleotide 280 to about nucleotide 318; the fragment of SEQ
ID NO:123 from about nucleotide 505 to about nucleotide 558; the
fragments of SEQ ID NO:124 from about nucleotide 1 to about
nucleotide 21 and from about nucleotide 694 to about nucleotide
720; the fragment of SEQ ID NO:125 from about nucleotide 331 to
about nucleotide 378; the fragment of SEQ ID NO:126 from about
nucleotide 1012 to about nucleotide 1047; the fragment of SEQ ID
NO:127 from about nucleotide 1070 to about nucleotide 1106; the
fragment of SEQ ID NO:128 from about nucleotide 133 to about
nucleotide 186; the fragment of SEQ ID NO:129 from about nucleotide
432 to about nucleotide 482; the fragments of SEQ ID NO:130 from
about nucleotide 1745 to about nucleotide 1795 and from about
nucleotide 1910 to about nucleotide 1979; the fragment of SEQ ID
NO:131 from about nucleotide 322 to about nucleotide 375; the
fragment of SEQ ID NO:132 from about nucleotide 147 to about
nucleotide 203; the fragment of SEQ ID NO:133 from about nucleotide
557 to about nucleotide 613; the fragment of SEQ ID NO:134 from
about nucleotide 509 to about nucleotide 595; the fragment of SEQ
ID NO:135 from about nucleotide 808 to about nucleotide 848; the
fragment of SEQ ID NO:136 from about nucleotide 216 to about
nucleotide 260; the fragment of SEQ ID NO:137 from about nucleotide
132 to about nucleotide 188; the fragment of SEQ ID NO:138 from
about nucleotide 231 to about nucleotide 278; the fragment of SEQ
ID NO:139 from about nucleotide 303 to about nucleotide 350; the
fragment of SEQ ID NO:140 from about nucleotide 507 to about
nucleotide 550; the fragment of SEQ ID NO:141 from about nucleotide
433 to about nucleotide 477; the fragment of SEQ ID NO:142 from
about nucleotide 266 to about nucleotide 314; the fragment of SEQ
ID:143 from about nucleotide 3 to about nucleotide 48; the fragment
of SEQ ID NO:144 from about nucleotide 76 to about nucleotide 122;
the fragment of SEQ ID NO:145 from about nucleotide 93 to about
nucleotide 139; the fragment of SEQ ID NO:146 from about nucleotide
241 to about nucleotide 286; the fragment of SEQ ID NO:147 from
about nucleotide 43 to about nucleotide 89; the fragment of SEQ ID
NO:148 from about nucleotide 219 to about nucleotide 265; the
fragment of SEQ ID NO:149 from about nucleotide 619 to about
nucleotide 663; the fragment of SEQ ID NO:150 from about nucleotide
25 to about nucleotide 69; the fragment of SEQ ID NO:151 from about
nucleotide 175 to about nucleotide 221; the fragment of SEQ ID
NO:152 from about nucleotide 94 to about nucleotide 138; the
fragment of SEQ ID NO:153 from about nucleotide 46 to about
nucleotide 90; the fragment of SEQ ID NO:154 from about nucleotide
1081 to about nucleotide 1127; the fragment of SEQ ID NO:155 from
about nucleotide 31 to about nucleotide 77; the fragment of SEQ ID
NO:156 from about nucleotide 157 to about nucleotide 201; the
fragment of SEQ ID NO:157 from about nucleotide 216 to about
nucleotide 259; and the fragment of SEQ ID NO:158 from about
nucleotide 517 to about nucleotide 561. The polypeptides encoded by
these fragments may be useful, for example, as antigenic
polypeptides.
[0104] The invention also encompasses HTMPN variants. A preferred
HTMPN variant is one which has at least about 80%, more preferably
at least about 90%, and most preferably at least about 95% amino
acid sequence identity to the HTMPN amino acid sequence, and which
contains at least one functional or structural characteristic of
HTMPN.
[0105] The invention also encompasses polynucleotides which encode
HTMPN. In a particular embodiment, the invention encompasses a
polynucleotide sequence comprising a sequence selected from the
group consisting of SEQ ID NO:80-158, which encodes HTMPN.
[0106] The invention also encompasses a variant of a polynucleotide
sequence encoding HTMPN. In particular, such a variant
polynucleotide sequence will have at least about 80%, more
preferably at least about 90%, and most preferably at least about
95% polynucleotide sequence identity to the polynucleotide sequence
encoding HTMPN. A particular aspect of the invention encompasses a
variant of a polynucleotide sequence comprising a sequence selected
from the group consisting of SEQ ID NO:80-158 which has at least
about 80%, more preferably at least about 90%, and most preferably
at least about 95% polynucleotide sequence identity to a nucleic
acid sequence selected from the group consisting of SEQ ID
NO:80-158. Any one of the polynucleotide variants described above
can encode an amino acid sequence which contains at least one
functional or structural characteristic of HTMPN.
[0107] It will be appreciated by those skilled in the art that as a
result of the degeneracy of the genetic code, a multitude of
polynucleotide sequences encoding HTMPN, some bearing minimal
similarity to the polynucleotide sequences of any known and
naturally occurring gene, may be produced. Thus, the invention
contemplates each and every possible variation of polynucleotide
sequence that could be made by selecting combinations based on
possible codon choices. These combinations are made in accordance
with the standard triplet genetic code as applied to the
polynucleotide sequence of naturally occurring HTMPN, and all such
variations are to be considered as being specifically
disclosed.
[0108] Although nucleotide sequences which encode HTMPN and its
variants are preferably capable of hybridizing to the nucleotide
sequence of the naturally occurring HTMPN under appropriately
selected conditions of stringency, it may be advantageous to
produce nucleotide sequences encoding HTMPN or its derivatives
possessing a substantially different codon usage, e.g., inclusion
of non-naturally occurring codons. Codons may be selected to
increase the rate at which expression of the peptide occurs in a
particular prokaryotic or eukaryotic host in accordance with the
frequency with which particular codons are utilized by the host.
Other reasons for substantially altering the nucleotide sequence
encoding HTMPN and its derivatives without altering the encoded
amino acid sequences include the production of RNA transcripts
having more desirable properties, such as a greater half-life, than
transcripts produced from the naturally occurring sequence.
[0109] The invention also encompasses production of DNA sequences
which encode HTMPN and HTMPN derivatives, or fragments thereof,
entirely by synthetic chemistry. After production, the synthetic
sequence may be inserted into any of the many available expression
vectors and cell systems using reagents well known in the art.
Moreover, synthetic chemistry may be used to introduce mutations
into a sequence encoding HTMPN or any fragment thereof.
[0110] Also encompassed by the invention are polynucleotide
sequences that are capable of hybridizing to the claimed
polynucleotide sequences, and, in particular, to those shown in SEQ
ID NO:80-158 and fragments thereof under various conditions of
stringency. (See, e.g., Wahl, G. M. and S. L. Berger (1987) Methods
Enzymol. 152:399-407; Kimmel, A. R. (1987) Methods Enzymol.
152:507-511.) For example, stringent salt concentration will
ordinarily be less than about 750 mM NaCl and 75 mM trisodium
citrate, preferably less than about 500 mM NaCl and 50 mM trisodium
citrate, and most preferably less than about 250 mM NaCl and 25 mM
trisodium citrate. Low stringency hybridization can be obtained in
the absence of organic solvent, e.g., formamide, while high
stringency hybridization can be obtained in the presence of at
least about 35% formamide, and most preferably at least about 50%
formamide. Stringent temperature conditions will ordinarily include
temperatures of at least about 30.degree. C., more preferably of at
least about 37.degree. C., and most preferably of at least about
42.degree. C. Varying additional parameters, such as hybridization
time, the concentration of detergent, e.g., sodium dodecyl sulfate
(SDS), and the inclusion or exclusion of carrier DNA, are well
known to those skilled in the art. Various levels of stringency are
accomplished by combining these various conditions as needed. In a
preferred embodiment, hybridization will occur at 30.degree. C. in
750 mM NaCl. 75 mM trisodium citrate, and 1% SDS. In a more
preferred embodiment, hybridization will occur at 37.degree. C. in
500 mM NaCl, 50 mM trisodium citrate, 1% SDS, 35% formamide, and
100 .mu.g/ml denatured salmon sperm DNA (ssDNA). In a most
preferred embodiment, hybridization will occur at 42.degree. C. in
250 mM NaCl, 25 mM trisodium citrate, 1% SDS. 50% formamide, and
200 .mu.g/ml ssDNA. Useful variations on these conditions will be
readily apparent to those skilled in the art.
[0111] The washing steps which follow hybridization can also vary
in stringency. Wash stringency conditions can be defined by salt
concentration and by temperature. As above, wash stringency can be
increased by decreasing salt concentration or by increasing
temperature. For example, stringent salt concentration for the wash
steps will preferably be less than about 30 mM NaCl and 3 mM
trisodium citrate, and most preferably less than about 15 mM NaCl
and 1.5 mM trisodium citrate. Stringent temperature conditions for
the wash steps will ordinarily include temperature of at least
about 25.degree. C., more preferably of at least about 42.degree.
C., and most preferably of at least about 68.degree. C. In a
preferred embodiment, wash steps will occur at 25.degree. C. in 30
mM NaCl, 3 mM trisodium citrate, and 0.1% SDS. In a more preferred
embodiment, wash steps will occur at 42.degree. C. in 15 mM NaCl,
1.5 mM trisodium citrate, and 0.1% SDS. In a most preferred
embodiment, wash steps will occur at 68.degree. C. in 15 mM NaCl,
1.5 mM trisodium citrate, and 0.1% SDS. Additional variations on
these conditions will be readily apparent to those skilled in the
art.
[0112] Methods for DNA sequencing are well known in the art and may
be used to practice any of the embodiments of the invention. The
methods may employ such enzymes as the Klenow fragment of DNA
polymerase I, SEQUENASE (US Biochemical, Cleveland Ohio), Taq
polymerase (Perkin-Elmer), thermostable T7 polymerase (Amersham
Pharmacia Biotech, Piscataway N.J.), or combinations of polymerases
and proofreading exonucleases such as those found in the ELONGASE
amplification system (Life Technologies, Gaithersburg Md.).
Preferably, sequence preparation is automated with machines such as
the Hamilton MICROLAB 2200 (Hamilton, Reno Nev.), Peltier Thermal
Cycler 200 (PTC200; MJ Research, Watertown Mass.) and the ABI
CATALYST 800 (Perkin-Elmer). Sequencing is then carried out using
either ABI 373 or 377 DNA sequencing systems (Perkin-Elmer) or the
MEGABACE 1000 DNA sequencing system (Molecular Dynamics, Sunnyvale
Calif.). The resulting sequences are analyzed using a variety of
algorithms which are well known in the art. (See, e.g., Ausubel, F.
M. (1997) Short Protocols in Molecular Biology, John Wiley &
Sons, New York N.Y., unit 7.7; Meyers, R. A. (1995) Molecular
Biology and Biotechnology, Wiley VCH, New York N.Y., pp.
856-853.)
[0113] The nucleic acid sequences encoding HTMPN may be extended
utilizing a partial nucleotide sequence and employing various
PCR-based methods known in the art to detect upstream sequences,
such as promoters and regulatory elements. For example, one method
which may be employed, restriction-site PCR, uses universal and
nested primers to amplify unknown sequence from genomic DNA within
a cloning vector. (See, e.g., Sarkar, G. (1993) PCR Methods Applic.
2:318-322.) Another method, inverse PCR, uses primers that extend
in divergent directions to amplify unknown sequence from a
circularized template. The template is derived from restriction
fragments comprising a known genomic locus and surrounding
sequences. (See, e.g., Triglia, T. et al. (1988) Nucleic Acids Res.
16:8186.) A third method, capture PCR, involves PCR amplification
of DNA fragments adjacent to known sequences in human and yeast
artificial chromosome DNA. (See, e.g., Lagerstrom, M. et al. (1991)
PCR Methods Applic. 1:111-119.) In this method, multiple
restriction enzyme digestions and ligations may be used to insert
an engineered double-stranded sequence into a region of unknown
sequence before performing PCR. Other methods which may be used to
retrieve unknown sequences are known in the art. (See, e.g.,
Parker, J. D. et al. (1991) Nucleic Acids Res. 19:3055-306).
Additionally, one may use PCR, nested primers, and PROMOTERFINDER
libraries (Clontech, Palo Alto Calif.) to walk genomic DNA. This
procedure avoids the need to screen libraries and is useful in
finding intron/exon junctions. For all PCR-based methods, primers
may be designed using commercially available software, such as
OLIGO 4.06 Primer Analysis software (National Biosciences, Plymouth
Minn.) or another appropriate program, to be about 22 to 30
nucleotides in length, to have a GC content of about 50% or more,
and to anneal to the template at temperatures of about 68.degree.
C. to 72.degree. C.
[0114] When screening for full-length cDNAs, it is preferable to
use libraries that have been size-selected to include larger cDNAs.
In addition, random-primed libraries, which often include sequences
containing the 5' regions of genes, are preferable for situations
in which an oligo d(T) library does not yield a full-length cDNA.
Genomic libraries may be useful for extension of sequence into 5'
non-transcribed regulatory regions.
[0115] Capillary electrophoresis systems which are commercially
available may be used to analyze the size or confirm the nucleotide
sequence of sequencing or PCR products. In particular, capillary
sequencing may employ flowable polymers for electrophoretic
separation, four different nucleotide-specific, laser-stimulated
fluorescent dyes, and a charge coupled device camera for detection
of the emitted wavelengths. Output/light intensity may be converted
to electrical signal using appropriate software (e.g., GENOTYPER
and SEQUENCE NAVIGATOR, Perkin-Elmer), and the entire process from
loading of samples to computer analysis and electronic data display
may be computer controlled. Capillary electrophoresis is especially
preferable for sequencing small DNA fragments which may be present
in limited amounts in a particular sample.
[0116] In another embodiment of the invention, polynucleotide
sequences or fragments thereof which encode HTMPN may be cloned in
recombinant DNA molecules that direct expression of HTMPN, or
fragments or functional equivalents thereof, in appropriate host
cells. Due to the inherent degeneracy of the genetic code, other
DNA sequences which encode substantially the same or a functionally
equivalent amino acid sequence may be produced and used to express
HTMPN.
[0117] The nucleotide sequences of the present invention can be
engineered using methods generally known in the art in order to
alter HTMPN-encoding sequences for a variety of purposes including,
but not limited to, modification of the cloning, processing, and/or
expression of the gene product. DNA shuffling by random
fragmentation and PCR reassembly of gene fragments and synthetic
oligonucleotides may be used to engineer the nucleotide sequences.
For example, oligonucleotide-mediated site-directed mutagenesis may
be used to introduce mutations that create new restriction sites,
alter glycosylation patterns, change codon preference, produce
splice variants, and so forth.
[0118] In another embodiment, sequences encoding HTMPN may be
synthesized, in to whole or in part, using chemical methods well
known in the art. (See, e.g., Caruthers, M. H. et al. (1980) Nucl.
Acids Res. Symp. Ser. 215-223, and Horn, T. et al. (1980) Nucl.
Acids Res. Symp. Ser. 225-232.) Alternatively, HTMPN itself or a
fragment thereof may be synthesized using chemical methods. For
example, peptide synthesis can be performed using various
solid-phase techniques. (See, e.g., Roberge, J. Y. et al. (1995)
Science 269:202-204.1 Automated synthesis may be achieved using the
ABI 431A Peptide Synthesizer (Perkin-Elmer). Additionally, the
amino acid sequence of HTMPN, or any part thereof, may be altered
during direct synthesis and/or combined with sequences from other
proteins, or any part thereof, to produce a variant
polypeptide.
[0119] The peptide may be substantially purified by preparative
high performance liquid chromatography. (See, e.g, Chiez, R. M. and
F. Z. Regnier (1990) Methods Enzymol. 182:392-421.) The composition
of the synthetic peptides may be confirmed by amino acid analysis
or by sequencing. (See, e.g., Creighton, T. (1984) Proteins,
Structures and Molecular Properties, WH Freeman, New York N.Y.)
[0120] In order to express a biologically active HTMPN, the
nucleotide sequences encoding HTMPN or derivatives thereof may be
inserted into an appropriate expression vector, i.e., a vector
which contains the necessary elements for transcriptional and
translational control of the inserted coding sequence in a suitable
host. These elements include regulatory sequences, such as
enhancers, constitutive and inducible promoters, and 5' and 3'
untranslated regions in the vector and in polynucleotide sequences
encoding HTMPN. Such elements may vary in their strength and
specificity. Specific initiation signals may also be used to
achieve more efficient translation of sequences encoding HTMPN.
Such signals include the ATG initiation codon and adjacent
sequences, e.g. the Kozak sequence. In cases where sequences
encoding HTMPN and its initiation codon and upstream regulatory
sequences are inserted into the appropriate expression vector, no
additional transcriptional or translational control signals may be
needed. However, in cases where only coding sequence, or a fragment
thereof, is inserted, exogenous translational control signals
including an in-frame ATG initiation codon should be provided by
the vector. Exogenous translational elements and initiation codons
may be of various origins, both natural and synthetic. The
efficiency of expression may be enhanced by the inclusion of
enhancers appropriate for the particular host cell system used.
(See, e.g., Scharf, D. et al. (1994) Results Probl. Cell Differ.
20:125-162.)
[0121] Methods which are well known to those skilled in the art may
be used to construct expression vectors containing sequences
encoding HTMPN and appropriate transcriptional and translational
control elements. These methods include in vitro recombinant DNA
techniques, synthetic techniques, and in vivo genetic
recombination. (See, e.g., Sambrook, J. et al. (1989) Molecular
Cloning, A Laboratory Manual, Cold Spring Harbor Press, Plainview
N.Y., ch. 4, 8, and 16-17; Ausubel, F. M. et al. (1995) Current
Protocols in Molecular Biology, John Wiley & Sons, New York
N.Y., ch. 9, 13, and 16.)
[0122] A variety of expression vector/host systems may be utilized
to contain and express sequences encoding HTMPN. These include, but
are not limited to, microorganisms such as bacteria transformed
with recombinant bacteriophage, plasmid, or cosmid DNA expression
vectors; yeast transformed with yeast expression vectors; insect
cell systems infected with viral expression vectors (e.g.,
baculovirus); plant cell systems transformed with viral expression
vectors (e.g., cauliflower mosaic virus, CaMV, or tobacco mosaic
virus, TMV) or with bacterial expression vectors (e.g., Ti or
pBR322 plasmids); or animal cell systems. The invention is not
limited by the host cell employed.
[0123] In bacterial systems, a number of cloning and expression
vectors may be selected depending upon the use intended for
polynucleotide sequences encoding HTMPN. For example, routine
cloning, subcloning, and propagation of polynucleotide sequences
encoding HTMPN can be achieved using a multifunctional E. coli
vector such as PBLUESCRIPT (Stratagene, La Jolla Calif.) or pSPORT1
plasmid (Life Technologies). Ligation of sequences encoding HTMPN
into the vector's multiple cloning site disrupts the lacZ gene,
allowing a colorimetric screening procedure for identification of
transformed bacteria containing recombinant molecules. In addition,
these vectors may be useful for in vitro transcription, dideoxy
sequencing, single strand rescue with helper phage, and creation of
nested deletions in the cloned sequence. (See, e.g., Van Heeke, G.
and S. M. Schuster (1989) J. Biol. Chem. 264:5503-5509.) When large
quantities of HTMPN are needed, e.g. for the production of
antibodies, vectors which direct high level expression of HTMPN may
be used. For example, vectors containing the strong, inducible T5
or T7 bacteriophage promoter may be used.
[0124] Yeast expression systems may be used for production of
HTMPN. A number of vectors containing constitutive or inducible
promoters, such as alpha factor, alcohol oxidase, and PGH, may be
used in the yeast Saccharomyces cerevisiae or Pichia pastoris. In
addition, such vectors direct either the secretion or intracellular
retention of expressed proteins and enable integration of foreign
sequences into the host genome for stable propagation. (See, e.g.
Ausubel, 1995, supra; Grant et al. (1987) Methods Enzymol.
153:516-54; and Scorer, C. A. et al. (1994) Bio/Technology
12:181-184.)
[0125] Plant systems may also be used for expression of HTMPN.
Transcription of sequences encoding HTMPN may be driven viral
promoters, e.g., the .sup.35S and 19S promoters of CaMV used alone
or in combination with the omega leader sequence from TMV
(Takamatsu, N. (1987) EMBO J. 6:307-311). Alternatively, plant
promoters such as the small subunit of RUBISCO or heat shock
promoters may be used. (See, e.g., Coruzzi, G. et al. (1984) EMBO
J. 3:1671-1680; Broglie, R. et al. (1984) Science 224:838-843; and
Winter, J. et al. (1991) Results Probl. Cell Differ. 17:85-105.)
These constructs can be introduced into plant cells by direct DNA
transformation or pathogen-mediated transfection. (See, e.g., The
McGraw Hill Yearbook of Science and Technology (1992) McGraw Hill,
New York N.Y., pp. 191-196.)
[0126] In mammalian cells, a number of viral-based expression
systems may be utilized. In cases where an adenovirus is used as an
expression vector, sequences encoding HTMPN may be ligated into an
adenovirus transcription/translation complex consisting of the late
promoter and tripartite leader sequence. Insertion in a
non-essential E1 or E3 region of the viral genome may be used to
obtain infective virus which expresses HTMPN in host cells. (See,
e.g., Logan, J. and T. Shenk (1984) Proc. Natl. Acad. Sci.
81:3655-3659.) In addition, transcription enhancers, such as the
Rous sarcoma virus (RSV) enhancer, may be used to increase
expression in mammalian host cells. SV40 or EBV-based vectors may
also be used for high-level protein expression.
[0127] Human artificial chromosomes (HACs) may also be employed to
deliver larger fragments of DNA than can be contained in and
expressed from a plasmid. HACs of about 6 kb to 10 Mb are
constructed and delivered via conventional delivery methods
(liposomes, polycationic amino polymers, or vesicles) for
therapeutic purposes. (See, e.g., Harrington, J. J. et al. (1997)
Nat. Genet. 15:345-355.)
[0128] For long term production of recombinant proteins in
mammalian systems, stable expression of HTMPN in cell lines is
preferred. For example, sequences encoding HTMPN can be transformed
into cell lines using expression vectors which may contain viral
origins of replication and/or endogenous expression elements and a
selectable marker gene on the same or on a separate vector.
Following the introduction of the vector, cells may be allowed to
grow for about 1 to 2 days in enriched media before being switched
to selective media. The purpose of the selectable marker is to
confer resistance to a selective agent, and its presence allows
growth and recovery of cells which successfully express the
introduced sequences. Resistant clones of stably transformed cells
may be propagated using tissue culture techniques appropriate to
the cell type.
[0129] Any number of selection systems may be used to recover
transformed cell lines. These include, but are not limited to, the
herpes simplex virus thymidine kinase and adenine
phosphoribosyltransferase genes, for use in tk.sup.- or apr.sup.-
cells, respectively. (See, e.g., Wigler. M. et al. (1977) Cell
11:223-232; Lowy, I. et al. (1980) Cell 22:817-823.) Also,
antimetabolite, antibiotic, or herbicide resistance can be used as
the basis for selection. For example, dhfr confers resistance to
methotrexate; neo confers resistance to the aminoglycosides,
neomycin and G-418; and als or pat confer resistance to
chlorsulfuron and phosphinotricin acetyltransferase, respectively.
(See, e.g., Wigler, M. et al. (1980) Proc. Natl. Acad. Sci.
77:3567-3570; Colbere-Garapin, F. et al. (1981) J. Mol. Biol.
150:1-14.) Additional selectable genes have been described, e.g.,
trpB and hisD, which alter cellular requirements for metabolites.
(See, e.g., Hartman, S. C. and R. C. Mulligan (1988) Proc. Natl.
Acad. Sci. 85:8047-8051.) Visible markers, e.g., anthocyanins,
green fluorescent proteins (GFP; Clontech), .beta. glucuronidase
and its substrate .beta.-glucuronide, or luciferase and its
substrate luciferin may be used. These markers can be used not only
to identify transformants, but also to quantify the amount of
transient or stable protein expression attributable to a specific
vector system. (See, e.g., Rhodes, C. A. (1995) Methods Mol. Biol.
55:121-131.)
[0130] Although the presence/absence of marker gene expression
suggests that the gene of interest is also present, the presence
and expression of the gene may need to be confirmed. For example,
if the sequence encoding HTMPN is inserted within a marker gene
sequence, transformed cells containing sequences encoding HTMPN can
be identified by the absence of marker gene function.
Alternatively, a marker gene can be placed in tandem with a
sequence encoding HTMPN under the control of a single promoter.
Expression of the marker gene in response to induction or selection
usually indicates expression of the tandem gene as well.
[0131] In general, host cells that contain the nucleic acid
sequence encoding HTMPN and that express HTMPN may be identified by
a variety of procedures known to those of skill in the art. These
procedures include, but are not limited to, DNA-DNA or DNA-RNA
hybridizations, PCR amplification, and protein bioassay or
immunoassay techniques which include membrane, solution, or chip
based technologies for the detection and/or quantification of
nucleic acid or protein sequences.
[0132] Immunological methods for detecting and measuring the
expression of HTMPN using either specific polyclonal or monoclonal
antibodies are known in the art. Examples of such techniques
include enzyme-linked immunosorbent assays (ELISAs),
radioimmunoassays (RIAs), and fluorescence activated cell sorting
(FACS). A two-site, monoclonal-based immunoassay utilizing
monoclonal antibodies reactive to two non-interfering epitopes on
HTMPN is preferred, but a competitive binding assay may be
employed. These and other assays are well known in the art. (See,
e.g., Hampton, R. et al. (1990) Serological Methods, a Laboratory
Manual, APS Press, St Paul Minn., Sect. IV; Coligan, J. E. et al.
(1997) Current Protocols in Immunology, Greene Pub. Associates and
Wiley-Interscience, New York N.Y.; and Pound, J. D. (1998)
Immunochemical Protocols, Humana Press, Totowa N.J.).
[0133] A wide variety of labels and conjugation techniques are
known by those skilled in the art and may be used in various
nucleic acid and amino acid assays. Means for producing labeled
hybridization or PCR probes for detecting sequences related to
polynucleotides encoding HTMPN include oligolabeling, nick
translation, end-labeling, or PCR amplification using a labeled
nucleotide. Alternatively, the sequences encoding HTMPN, or any
fragments thereof, may be cloned into a vector for the production
of an mRNA probe. Such vectors are known in the art, are
commercially available, and may be used to synthesize RNA probes in
vitro by addition of an appropriate RNA polymerase such as T7, T3,
or SP6 and labeled nucleotides. These procedures may be conducted
using a variety of commercially available kits, such as those
provided by Amersham Pharmacia Biotech, Promega (Madison Wis.), and
US Biochemical. Suitable reporter molecules or labels which may be
used for ease of detection include radionuclides, enzymes,
fluorescent, chemiluminescent, or chromogenic agents, as well as
substrates, cofactors, inhibitors, magnetic particles, and the
like.
[0134] Host cells transformed with nucleotide sequences encoding
HTMPN may be cultured under conditions suitable for the expression
and recovery of the protein from cell culture. The protein produced
by a transformed cell may be secreted or retained intracellularly
depending on the sequence and/or the vector used. As will be
understood by those of skill in the art, expression vectors
containing polynucleotides which encode HTMPN may be designed to
contain signal sequences which direct secretion of HTMPN through a
prokaryotic or eukaryotic cell membrane.
[0135] In addition, a host cell strain may be chosen for its
ability to modulate expression of the inserted sequences or to
process the expressed protein in the desired fashion. Such
modifications of the polypeptide include, but are not limited to,
acetylation, carboxylation, glycosylation, phosphorylation,
lipidation, and acylation. Post-translational processing which
cleaves a "prepro" form of the protein may also be used to specify
protein targeting, folding, and/or activity. Different host cells
which have specific cellular machinery and characteristic
mechanisms for post-translational activities (e.g., CHO, HeLa,
MDCK, HEK293, and WI38), are available from the American Type
Culture Collection (ATCC, Bethesda Md.) and may be chosen to ensure
the correct modification and processing of the foreign protein.
[0136] In another embodiment of the invention, natural, modified,
or recombinant nucleic acid sequences encoding HTMPN may be ligated
to a heterologous sequence resulting in translation of a fusion
protein in any of the aforementioned host systems. For example, a
chimeric HTMPN protein containing a heterologous moiety that can be
recognized by a commercially available antibody may facilitate the
screening of peptide libraries for inhibitors of HTMPN activity.
Heterologous protein and peptide moieties may also facilitate
purification of fusion proteins using commercially available
affinity matrices. Such moieties include, but are not limited to,
glutathione S-transferase (GST), maltose binding protein (MBP),
thioredoxin (Trx), calmodulin binding peptide (CBP), 6-His, FLAG,
c-myc, and hemagglutinin (HA). GST, MBP, Trx, CBP, and 6-His enable
purification of their cognate fusion proteins on immobilized
glutathione, maltose, phenylarsine oxide, calmodulin, and
metal-chelate resins, respectively. FLAG, c-myc, and hemagglutinin
(HA) enable immunoaffinity purification of fusion proteins using
commercially available monoclonal and polyclonal antibodies that
specifically recognize these epitope tags. A fusion protein may
also be engineered to contain a proteolytic cleavage site located
between the HTMPN encoding sequence and the heterologous protein
sequence, so that HTMPN may be cleaved away from the heterologous
moiety following purification. Methods for fusion protein
expression and purification are discussed in Ausubel (1995, supra,
ch 10). A variety of commercially available kits may also be used
to facilitate expression and purification of fusion proteins.
[0137] In a further embodiment of the invention, synthesis of
radiolabeled HTMPN may be achieved in vitro using the TNT rabbit
reticulocyte lysate or wheat germ extract systems (Promega). These
systems couple transcription and translation of protein-coding
sequences operably associated with the T7, T3, or SP6 promoters.
Translation takes place in the presence of a radiolabeled amino
acid precursor, preferably .sup.35S-methionine.
[0138] Fragments of HTMPN may be produced not only by recombinant
production, but also by direct peptide synthesis using solid-phase
techniques. (See, e.g., Creighton, supra, pp. 55-60.) Protein
synthesis may be performed by manual techniques or by automation.
Automated synthesis may be achieved, for example, using the ABI
431A Peptide Synthesizer (Perkin-Elmer). Various fragments of HTMPN
may be synthesized separately and then combined to produce the full
length molecule.
Therapeutics
[0139] Chemical and structural similarity, e.g., in the context of
sequences and motifs, exists between regions of HTMPN and human
transmembrane proteins. In addition, the expression of HTMPN is
closely associated with tissue involved in inflammation and the
immune response and with cell proliferative conditions including
cancer, and in reproductive, gastrointestinal fetal, smooth muscle,
cardiovascular, developmental, and nervous tissue. Therefore, HTMPN
appears to play a role in immune, reproductive, smooth muscle,
neurological, gastrointestinal, developmental, and cell
proliferative disorders. In the treatment of immune, reproductive,
smooth muscle, neurological, gastrointestinal, developmental, and
cell proliferative disorders associated with increased HTMPN
expression or activity, it is desirable to decrease the expression
or activity of HTMPN. In the treatment of the above conditions
associated with decreased HTMPN expression or activity, it is
desirable to increase the expression or activity of HTMPN.
[0140] Therefore, in one embodiment, HTMPN or a fragment or
derivative thereof may be administered to a subject to treat or
prevent a disorder associated with decreased expression or activity
of HTMPN. Examples of such disorders include, but are not limited
to, an immune disorder such as acquired immunodeficiency syndrome
(AIDS), Addison's disease, adult respiratory distress syndrome,
allergies, ankylosing spondylitis, amyloidosis, anemia, asthma,
atherosclerosis, autoimmune hemolytic anemia, autoimmune
thyroiditis, autoimmune polyenodocrinopathy-candidiasis-ectodermal
dystrophy (APECED), bronchitis, cholecystitis, contact dermatitis,
Crohn's disease, atopic dermatitis, dermatomyositis, diabetes
mellitus, emphysema, episodic lymphopenia with lymphocytotoxins,
erythroblastosis fetalis, erythema nodosum, atrophic gastritis,
glomerulonephritis, Goodpasture's syndrome, gout, Graves' disease,
Hashimoto's thyroiditis, hypereosinophilia, irritable bowel
syndrome, multiple sclerosis, myasthenia gravis, myocardial or
pericardial inflammation, osteoarthritis, osteoporosis,
pancreatitis, polymyositis, psoriasis, Reiter's syndrome,
rheumatoid arthritis, scleroderma, Sjogren's syndrome, systemic
anaphylaxis, systemic lupus erythematosus, systemic sclerosis,
thrombocytopenic purpura, ulcerative colitis, uveitis, Werner
syndrome, complications of cancer, hemodialysis, and extracorporeal
circulation, viral, bacterial, fungal, parasitic, protozoal, and
helminthic infections, and trauma; a reproductive disorder such as
a disorder of prolactin production; infertility, including tubal
disease, ovulatory defects, and endometriosis; a disruption of the
estrous cycle, a disruption of the menstrual cycle, polycystic
ovary syndrome, ovarian hyperstimulation syndrome, endometrial and
ovarian tumors, uterine fibroids, autoimmune disorders, ectopic
pregnancies, and teratogenesis; cancer of the breast, fibrocystic
breast disease, and galactorrhea; disruptions of spermatogenesis,
abnormal sperm physiology, cancer of the testis, cancer of the
prostate, benign prostatic hyperplasia, prostatitis, Peyronie's
disease, impotence, carcinoma of the male breast, and gynecomastia;
a smooth muscle disorder such as angina, anaphylactic shock,
arrhythmias, asthma, cardiovascular shock, Cushing's syndrome,
hypertension, hypoglycemia, myocardial infarction, migraine, and
pheochromocytoma, and myopathies including cardiomyopathy,
encephalopathy, epilepsy, Kearns-Sayre syndrome, lactic acidosis,
myoclonic disorder, and ophthalmoplegia; a neurological disorder
such as epilepsy, ischemic cerebrovascular disease, stroke,
cerebral neoplasms, Alzheimer's disease, Pick's disease.
Huntington's disease, dementia, Parkinson's disease and other
extrapyramidal disorders, amyotrophic lateral sclerosis and other
motor neuron disorders, progressive neural muscular atrophy,
retinitis pigmentosa, hereditary ataxias, multiple sclerosis and
other demyelinating diseases, bacterial and viral meningitis, brain
abscess, subdural empyema, epidural abscess, suppurative
intracranial thrombophlebitis, myelitis and radiculitis, viral
central nervous system disease; prion diseases including kuru,
Creutzfeldt-Jakob disease, and Gerstmann-Straussler-Scheinker
syndrome; fatal familial insomnia, nutritional and metabolic
diseases of the nervous system, neurofibromatosis, tuberous
sclerosis, cerebelloretinal hemangioblastomatosis,
encephalotrigeminal syndrome, mental retardation and other
developmental disorders of the central nervous system, cerebral
palsy, neuroskeletal disorders, autonomic nervous system disorders,
cranial nerve disorders, spinal cord diseases, muscular dystrophy
and other neuromuscular disorders, peripheral nervous system
disorders, dermatomyositis and polymyositis; inherited, metabolic,
endocrine, and toxic myopathies; myasthenia gravis, periodic
paralysis; mental disorders including mood, anxiety, and
schizophrenic disorders; akathesia, amnesia, catatonia, diabetic
neuropathy, tardive dyskinesia, dystonias, paranoid psychoses,
postherpetic neuralgia, and Tourette's disorder; a gastrointestinal
disorder such as dysphagia, peptic esophagitis, esophageal spasm,
esophageal stricture, esophageal carcinoma, dyspepsia, indigestion,
gastritis, gastric carcinoma, anorexia, nausea, emesis,
gastroparesis, antral or pyloric edema, abdominal angina, pyrosis,
gastroenteritis, intestinal obstruction, infections of the
intestinal tract, peptic ulcer, cholelithiasis, cholecystitis,
cholestasis, pancreatitis, pancreatic carcinoma, biliary tract
disease, hepatoma, infectious colitis, ulcerative colitis,
ulcerative proctitis, Crohn's disease, Whipple's disease,
Mallory-Weiss syndrome, colonic carcinoma, colonic obstruction,
irritable bowel syndrome, short bowel syndrome, diarrhea,
constipation, gastrointestinal hemorrhage, and acquired
immunodeficiency syndrome (AIDS) enteropathy, cirrhosis, jaundice,
cholestasis, hereditary hyperbilirubinemia, hepatic encephalopathy,
hepatorenal syndrome, hepatitis, hepatic steatosis,
hemochromatosis, Wilson's disease, .alpha..sub.1-antitrypsin
deficiency, Reye's syndrome, primary sclerosing cholangitis, liver
infarction, portal vein obstruction and thrombosis, passive
congestion, centrilobular necrosis, peliosis hepatis, hepatic vein
thrombosis, veno-occlusive disease, preeclampsia, eclampsia, acute
fatty liver of pregnancy, intrahepatic cholestasis of pregnancy,
and hepatic tumors including nodular hyperplasias, adenomas, and
carcinomas; a cell proliferative disorder such as actinic
keratosis, arteriosclerosis, atherosclerosis, bursitis, cirrhosis,
hepatitis, mixed connective tissue disease (MCTD), myelofibrosis,
paroxysmal nocturnal hernoglobinuria, polycythemia vera, psoriasis,
primary thrombocythemia, and cancers including adenocarcinoma,
leukemia, lymphoma, melanoma, myeloma, sarcoma, teratocarcinoma,
and, in particular, cancers of the adrenal gland, bladder, bone,
bone marrow, brain, breast, Do cervix, gall bladder, ganglia,
gastrointestinal tract, heart, kidney, liver, lung, muscle, ovary,
pancreas, parathyroid, penis, prostate, salivary glands, skin,
spleen, testis, thymus, thyroid, and uterus; and a developmental
disorder including, but not limited to, those listed above.
[0141] In another embodiment, a vector capable of expressing HTMPN
or a fragment or derivative thereof may be administered to a
subject to treat or prevent a disorder associated with decreased
expression or activity of HTMPN including, but not limited to,
those described above.
[0142] In a further embodiment, a pharmaceutical composition
comprising a substantially purified HTMPN in conjunction with a
suitable pharmaceutical carrier may be administered to a subject to
treat or prevent a disorder associated with decreased expression or
activity of HTMPN including, but not limited to, those provided
above.
[0143] In still another embodiment, an agonist which modulates the
activity of HTMPN may be administered to a subject to treat or
prevent a disorder associated with decreased expression or activity
of HTMPN including, but not limited to, those listed above.
[0144] In a further embodiment, an antagonist of HTMPN may be
administered to a subject to treat or prevent a disorder associated
with increased expression or activity of HTMPN. Examples of such
disorders include, but are not limited to, those described above.
In one aspect, an antibody which specifically binds HTMPN may be
used directly as an antagonist or indirectly as a targeting or
delivery mechanism for bringing a pharmaceutical agent to cells or
tissue which express HTMPN.
[0145] In an additional embodiment, a vector expressing the
complement of the polynucleotide encoding HTMPN may be administered
to a subject to treat or prevent a disorder associated with
increased expression or activity of HTMPN including, but not
limited to, those described above.
[0146] In other embodiments, any of the proteins, antagonists,
antibodies, agonists, complementary sequences, or vectors of the
invention may be administered in combination with other appropriate
therapeutic agents. Selection of the appropriate agents for use in
combination therapy may be made by one of ordinary skill in the
art, according to conventional pharmaceutical principles. The
combination of therapeutic agents may act synergistically to effect
the treatment or prevention of the various disorders described
above. Using this approach, one may be able to achieve therapeutic
efficacy with lower dosages of each agent, thus reducing the
potential for adverse side effects.
[0147] An antagonist of HTMPN may be produced using methods which
are generally known in the art. In particular, purified HTMPN may
be used to produce antibodies or to screen libraries of
pharmaceutical agents to identify those which specifically bind
HTMPN. Antibodies to HTMPN may also be generated using methods that
are well known in the art. Such antibodies may include, but are not
limited to, polyclonal, monoclonal, chimeric, and single chain
antibodies, Fab fragments, and fragments produced by a Fab
expression library. Neutralizing antibodies (i.e., those which
inhibit dimer formation) are especially preferred for therapeutic
use.
[0148] For the production of antibodies, various hosts including
goats, rabbits, rats, mice, humans, and others may be immunized by
injection with HTMPN or with any fragment or oligopeptide thereof
which has immunogenic properties. Depending on the host species,
various adjuvants may be used to increase immunological response.
Such adjuvants include, but are not limited to, Freund's, mineral
gels such as aluminum hydroxide, and surface active substances such
as lysolecithin, pluronic polyols, polyanions, peptides, oil
emulsions, KLH, and dinitrophenol. Among adjuvants used in humans,
BCG (bacilli Calmette-Guerin) and Corynebacterium parvum are
especially preferable.
[0149] It is preferred that the oligopeptides, peptides, or
fragments used to induce antibodies to HTMPN have an amino acid
sequence consisting of at least about 5 amino acids, and, more
preferably, of at least about 10 amino acids. It is also preferable
that these oligopeptides, peptides, or fragments are identical to a
portion of the amino acid sequence of the natural protein and
contain the entire amino acid sequence of a small, naturally
occurring molecule. Short stretches of HTMPN amino acids may be
fused with those of another protein, such as KLH, and antibodies to
the chimeric molecule may be produced.
[0150] Monoclonal antibodies to HTMPN may be prepared using any
technique which provides for the production of antibody molecules
by continuous cell lines in culture. These include, but are not
limited to, the hybridoma technique, the human B-cell hybridoma
technique, and the EBV-hybridoma technique. (See, e.g., Kohler, G.
et al. (1975) Nature 256:495-497; Kozbor, D. et al. (1985) J.
Immunol. Methods 81:31-42; Cote, R. J. et al. (1983) Proc. Natl.
Acad. Sci. 80:2026-2030; and Cole, S. P. et al. (1984) Mol. Cell.
Biol. 62:109-120.)
[0151] In addition, techniques developed for the production of
"chimeric antibodies," such as the splicing of mouse antibody genes
to human antibody genes to obtain a molecule with appropriate
antigen specificity and biological activity, can be used. (See,
e.g., Morrison, S. L. et al. (1984) Proc. Natl. Acad. Sci.
81:6851-6855; Neuberger, M. S. et al. (1984) Nature 312:604-608;
and Takeda, S. et al. (1985) Nature 314:452-454.) Alternatively,
techniques described for the production of single chain antibodies
may be adapted, using methods known in the art, to produce
HTMPN-specific single chain antibodies. Antibodies with related
specificity, but of distinct idiotypic composition, may be
generated by chain shuffling from random combinatorial
immunoglobulin libraries. (See, e.g., Burton D. R. (1991) Proc.
Natl. Acad. Sci. 88:10134-10137.)
[0152] Antibodies may also be produced by inducing in vivo
production in the lymphocyte population or by screening
immunoglobulin libraries or panels of highly specific binding
reagents as disclosed in the literature. (See, e.g., Orlandi, R. et
al. (1989) Proc. Natl. Acad. Sci. 86: 3833-3837; Winter, G. et al.
(1991) Nature 349:293-299.)
[0153] Antibody fragments which contain specific binding sites for
HTMPN may also be generated. For example, such fragments include,
but are not limited to, F(ab')2 fragments produced by pepsin
digestion of the antibody molecule and Fab fragments generated by
reducing the disulfide bridges of the F(ab')2 fragments.
Alternatively, Fab expression libraries may be constructed to allow
rapid and easy identification of monoclonal Fab fragments with the
desired specificity. (See, e.g., Huse, W. D. et al. (1989) Science
246:1275-1281.)
[0154] Various immunoassays may be used for screening to identify
antibodies having the desired specificity. Numerous protocols for
competitive binding or immunoradiometric assays using either
polyclonal or monoclonal antibodies with established specificities
are well known in the art. Such immunoassays typically involve the
measurement of complex formation between HTMPN and its specific
antibody. A two-site, monoclonal-based immunoassay utilizing
monoclonal antibodies reactive to two non-interfering HTMPN
epitopes is preferred, but a competitive binding assay may also be
employed (Pound, supra).
[0155] Various methods such as Scatchard analysis in conjunction
with radioimmunoassay techniques may be used to assess the affinity
of antibodies for HTMPN. Affinity is expressed as an association
constant, K.sub.a, which is defined as the molar concentration of
HTMPN-antibody complex divided by the molar concentrations of free
antigen and free antibody under equilibrium conditions. The K.sub.a
determined for a preparation of polyclonal antibodies, which are
heterogeneous in their affinities for multiple HTMPN epitopes,
represents the average affinity, or avidity, of the antibodies for
HTMPN. The K.sub.a determined for a preparation of monoclonal
antibodies, which are monospecific for a particular HTMPN epitope,
represents a true measure of affinity. High-affinity antibody
preparations with K.sub.a ranging from about 10.sup.9 to 10.sup.12
L/mole are preferred for use in immunoassays in which the
HTMPN-antibody complex must withstand rigorous manipulations.
Low-affinity antibody preparations with K.sub.a ranging from about
10.sup.6 to 10.sup.7 L/mole are preferred for use in
immunopurification and similar procedures which ultimately require
dissociation of HTMPN, preferably in active form, from the antibody
(Catty, D. (1988) Antibodies, Volume I: A Practical Approach, IRL
Press, Washington, D.C.; Liddell. J. E. and Cryer, A. (1991) A
Practical Guide to Monoclonal Antibodies, John Wiley & Sons,
New York N.Y.).
[0156] The titer and avidity of polyclonal antibody preparations
may be further evaluated to determine the quality and suitability
of such preparations for certain downstream applications. For
example, a polyclonal antibody preparation containing at least 1-2
mg specific antibody/ml, preferably 5-10 mg specific antibody/ml,
is preferred for use in procedures requiring precipitation of
HTMPN-antibody complexes. Procedures for evaluating antibody
specificity, titer, and avidity, and guidelines for antibody
quality and usage in various applications, are generally available.
(See, e.g., Catty, supra, and Coligan et al. supra.)
[0157] In another embodiment of the invention, the polynucleotides
encoding HTMPN, or any fragment or complement thereof, may be used
for therapeutic purposes. In one aspect, the complement of the
polynucleotide encoding HTMPN may be used in situations in which it
would be desirable to block the transcription of the mRNA. In
particular, cells may be transformed with sequences complementary
to polynucleotides encoding HTMPN. Thus, complementary molecules or
fragments may be used to modulate HTMPN activity, or to achieve
regulation of gene function. Such technology is now well known in
the art, and sense or antisense oligonucleotides or larger
fragments can be designed from various locations along the coding
or control regions of sequences encoding HTMPN.
[0158] Expression vectors derived from retroviruses, adenoviruses,
or herpes or vaccinia viruses, or from various bacterial plasmids,
may be used for delivery of nucleotide sequences to the targeted
organ, tissue, or cell population. Methods which are well known to
those skilled in the art can be used to construct vectors to
express nucleic acid sequences complementary to the polynucleotides
encoding HTMPN. (See, e.g., Sambrook, supra; Ausubel, 1995,
supra.)
[0159] Genes encoding HTMPN can be turned off by transforming a
cell or tissue with expression vectors which express high levels of
a polynucleotide, or fragment thereof, encoding HTMPN. Such
constructs may be used to introduce untranslatable sense or
antisense sequences into a cell. Even in the absence of integration
into the DNA, such vectors may continue to transcribe RNA molecules
until they are disabled by endogenous nucleases. Transient
expression may last for a month or more with a non-replicating
vector, and may last even longer if appropriate replication
elements are part of the vector system.
[0160] As mentioned above, modifications of gene expression can be
obtained by designing complementary sequences or antisense
molecules (DNA, RNA, or PNA) to the control, 5', or regulatory
regions of the gene encoding HTMPN. Oligonucleotides derived from
the transcription initiation site, e.g., between about positions
-10 and +10 from the start site, are preferred. Similarly,
inhibition can be achieved using triple helix base-pairing
methodology. Triple helix pairing is useful because it causes
inhibition of the ability of the double helix to open sufficiently
for the binding of polymerases, transcription factors, or
regulatory molecules. Recent therapeutic advances using triplex DNA
have been described in the literature. (See, e.g., Gee, J. E. et
al. (1994) in Huber, B. E. and B. I. Carr, Molecular and
Immunologic Approaches, Futura Publishing, Mt. Kisco N.Y., pp.
163-177.) A complementary sequence or antisense molecule may also
be designed to block translation of mRNA by preventing the
transcript from binding to ribosomes.
[0161] Ribozymes, enzymatic RNA molecules, may also be used to
catalyze the specific cleavage of RNA. The mechanism of ribozyme
action involves sequence-specific hybridization of the ribozyme
molecule to complementary target RNA, followed by endonucleolytic
cleavage. For example, engineered hammerhead motif ribozyme
molecules may specifically and efficiently catalyze endonucleolytic
cleavage of sequences encoding HTMPN.
[0162] Specific ribozyme cleavage sites within any potential RNA
target are initially identified by scanning the target molecule for
ribozyme cleavage sites, including the following sequences: GUA,
GUU, and GUC. Once identified, short RNA sequences of between 15
and 20 ribonucleotides, corresponding to the region of the target
gene containing the cleavage site, may be evaluated for secondary
structural features which may render the oligonucleotide
inoperable. The suitability of candidate targets may also be
evaluated by testing accessibility to hybridization with
complementary oligonucleotides using ribonuclease protection
assays.
[0163] Complementary ribonucleic acid molecules and ribozymes of
the invention may be prepared by any method known in the art for
the synthesis of nucleic acid molecules. These include techniques
for chemically synthesizing oligonucleotides such as solid phase
phosphoramidite chemical synthesis. Alternatively, RNA molecules
may be generated by in vitro and in vivo transcription of DNA
sequences encoding HTMPN. Such DNA sequences may be incorporated
into a wide variety of vectors with suitable RNA polymerase
promoters such as T7 or SP6. Alternatively, these cDNA constructs
that synthesize complementary RNA, constitutively or inducibly, can
be introduced into cell lines, cells, or tissues.
[0164] RNA molecules may be modified to increase intracellular
stability and half-life. Possible modifications include, but are
not limited to, the addition of flanking sequences at the 5' and/or
3' ends of the molecule, or the use of phosphorothioate or 2'
O-methyl rather than phosphodiesterase linkages within the backbone
of the molecule. This concept is inherent in the production of PNAs
and can be extended in all of these molecules by the inclusion of
nontraditional bases such as inosine, queosine, and wybutosine, as
well as acetyl-, methyl-, thio-, and similarly modified forms of
adenine, cytidine, guanine, thymine, and uridine which are not as
easily recognized by endogenous endonucleases.
[0165] Many methods for introducing vectors into cells or tissues
are available and equally suitable for use in vivo, in vitro, and
ex vivo. For ex vivo therapy, vectors may be introduced into stem
cells taken from the patient and clonally propagated for autologous
transplant back into that same patient. Delivery by transfection,
by liposome injections, or by polycationic amino polymers may be
achieved using methods which are well known in the art. (See. e.g.,
Goldman, C. K. et al. (1997) Nature Biotechnology 15:462-466.)
[0166] Any of the therapeutic methods described above may be
applied to any subject in need of such therapy, including, for
example, mammals such as dogs, cats, cows, horses, rabbits,
monkeys, and most preferably, humans.
[0167] An additional embodiment of the invention relates to the
administration of a pharmaceutical or sterile composition, in
conjunction with a pharmaceutically acceptable carrier, for any of
the therapeutic effects discussed above. Such pharmaceutical
compositions may consist of HTMPN, antibodies to HTMPN, and
mimetics, agonists, antagonists, or inhibitors of HTMPN. The
compositions may be administered alone or in combination with at
least one other agent, such as a stabilizing compound, which may be
administered in any sterile, biocompatible pharmaceutical carrier
including, but not limited to, saline, buffered saline, dextrose,
and water. The compositions may be administered to a patient alone,
or in combination with other agents, drugs, or hormones.
[0168] The pharmaceutical compositions utilized in this invention
may be administered by any number of routes including, but not
limited to, oral, intravenous, intramuscular, intra-arterial,
intramedullary, intrathecal, intraventricular, transdermal,
subcutaneous, intraperitoneal, intranasal, enteral, topical,
sublingual, or rectal means.
[0169] In addition to the active ingredients, these pharmaceutical
compositions may contain suitable pharmaceutically-acceptable
carriers comprising excipients and auxiliaries which facilitate
processing of the active compounds into preparations which can be
used pharmaceutically. Further details on techniques for
formulation and administration may be found in the latest edition
of Remington's Pharmaceutical Sciences (Maack Publishing, Easton
Pa.).
[0170] Pharmaceutical compositions for oral administration can be
formulated using pharmaceutically acceptable carriers well known in
the art in dosages suitable for oral administration. Such carriers
enable the pharmaceutical compositions to be formulated as tablets,
pills, dragees, capsules, liquids, gels, syrups, slurries,
suspensions, and the like, for ingestion by the patient.
[0171] Pharmaceutical preparations for oral use can be obtained
through combining active compounds with solid excipient and
processing the resultant mixture of granules (optionally, after
grinding) to obtain tablets or dragee cores. Suitable auxiliaries
can be added, if desired. Suitable excipients include carbohydrate
or protein fillers, such as sugars, including lactose, sucrose,
mannitol, and sorbitol; starch from corn, wheat, rice, potato, or
other plants; cellulose, such as methyl cellulose,
hydroxypropylmethyl-cellulose, or sodium carboxymethylcellulose;
gums, including arabic and tragacanth; and proteins, such as
gelatin and collagen. If desired, disintegrating or solubilizing
agents may be added, such as the cross-linked polyvinyl
pyrrolidone, agar, and alginic acid or a salt thereof, such as
sodium alginate.
[0172] Dragee cores may be used in conjunction with suitable
coatings, such as concentrated sugar solutions, which may also
contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel,
polyethylene glycol, and/or titanium dioxide, lacquer solutions,
and suitable organic solvents or solvent mixtures. Dyestuffs or
pigments may be added to the tablets or dragee coatings for product
identification or to characterize the quantity of active compound,
i.e., dosage.
[0173] Pharmaceutical preparations which can be used orally include
push-fit capsules made of gelatin, as well as soft, sealed capsules
made of gelatin and a coating, such as glycerol or sorbitol.
Push-fit capsules can contain active ingredients mixed with fillers
or binders, such as lactose or starches, lubricants, such as talc
or magnesium stearate, and, optionally, stabilizers. In soft
capsules, the active compounds may be dissolved or suspended in
suitable liquids, such as fatty oils, liquid, or liquid
polyethylene glycol with or without stabilizers.
[0174] Pharmaceutical formulations suitable for parenteral
administration may be formulated in aqueous solutions, preferably
in physiologically compatible buffers such as Hanks' solution,
Ringer's solution, or physiologically buffered saline. Aqueous
injection suspensions may contain substances which increase the
viscosity of the suspension, such as sodium carboxymethyl
cellulose, sorbitol, or dextran. Additionally, suspensions of the
active compounds may be prepared as appropriate oily injection
suspensions. Suitable lipophilic solvents or vehicles include fatty
oils, such as sesame oil, or synthetic fatty acid esters, such as
ethyl oleate, triglycerides, or liposomes. Non-lipid polycationic
amino polymers may also be used for delivery. Optionally, the
suspension may also contain suitable stabilizers or agents to
increase the solubility of the compounds and allow for the
preparation of highly concentrated solutions.
[0175] For topical or nasal administration, penetrants appropriate
to the particular barrier to be permeated are used in the
formulation. Such penetrants are generally known in the art.
[0176] The pharmaceutical compositions of the present invention may
be manufactured in a manner that is known in the art, e.g., by
means of conventional mixing, dissolving, granulating,
dragee-making, levigating, emulsifying, encapsulating, entrapping,
or lyophilizing processes.
[0177] The pharmaceutical composition may be provided as a salt and
can be formed with many acids, including but not limited to,
hydrochloric, sulfuric, acetic, lactic, tartaric, malic, and
succinic acid. Salts tend to be more soluble in aqueous or other
protonic solvents than are the corresponding free base forms. In
other cases, the preferred preparation may be a lyophilized powder
which may contain any or all of the following: 1 mM to 50 mM
histidine, 0.1% to 2% sucrose, and 2% to 7% mannitol, at a pH range
of 4.5 to 5.5, that is combined with buffer prior to use.
[0178] After pharmaceutical compositions have been prepared, they
can be placed in an appropriate container and labeled for treatment
of an indicated condition. For administration of HTMPN, such
labeling would include amount, frequency, and method of
administration.
[0179] Pharmaceutical compositions suitable for use in the
invention include compositions wherein the active ingredients are
contained in an effective amount to achieve the intended purpose.
The determination of an effective dose is well within the
capability of those skilled in the art.
[0180] For any compound, the therapeutically effective dose can be
estimated initially either in cell culture assays, e.g., of
neoplastic cells or in animal models such as mice, rats, rabbits,
dogs, or pigs. An animal model may also be used to determine the
appropriate concentration range and route of administration. Such
information can then be used to determine useful doses and routes
for administration in humans.
[0181] A therapeutically effective dose refers to that amount of
active ingredient, for example HTMPN or fragments thereof,
antibodies of HTMPN, and agonists, antagonists or inhibitors of
HTMPN, which ameliorates the symptoms or condition. Therapeutic
efficacy and toxicity may be determined by standard pharmaceutical
procedures in cell cultures or with experimental animals, such as
by calculating the ED.sub.50 (the dose therapeutically effective in
50% of the population) or LD.sub.50 (the dose lethal to 50% of the
population) statistics. The dose ratio of toxic to therapeutic
effects is the therapeutic index, and it can be expressed as the
LD.sub.50/ED.sub.50 ratio. Pharmaceutical compositions which
exhibit large therapeutic indices are preferred. The data obtained
from cell culture assays and animal studies are used to formulate a
range of dosage for human use. The dosage contained in such
compositions is preferably within a range of circulating
concentrations that includes the ED.sub.50 with little or no
toxicity. The dosage varies within this range depending upon the
dosage form employed, the sensitivity of the patient, and the route
of administration.
[0182] The exact dosage will be determined by the practitioner, in
light of factors related to the subject requiring treatment. Dosage
and administration are adjusted to provide sufficient levels of the
active moiety or to maintain the desired effect. Factors which may
be taken into account include the severity of the disease state,
the general health of the subject, the age, weight, and gender of
the subject, time and frequency of administration, drug
combination(s), reaction sensitivities, and response to therapy.
Long-acting pharmaceutical compositions may be administered every 3
to 4 days, every week, or biweekly depending on the half-life and
clearance rate of the particular formulation.
[0183] Normal dosage amounts may vary from about 0.1 .mu.g to
100,000 .mu.g, up to a total dose of about 1 gram, depending upon
the route of administration. Guidance as to particular dosages and
methods of delivery is provided in the literature and generally
available to practitioners in the art. Those skilled in the art
will employ different formulations for nucleotides than for
proteins or their inhibitors. Similarly, delivery of
polynucleotides or polypeptides will be specific to particular
cells, conditions, locations, etc.
Diagnostics
[0184] In another embodiment, antibodies which specifically bind
HTMPN may be used for the diagnosis of disorders characterized by
expression of HTMPN, or in assays to monitor patients being treated
with HTMPN or agonists, antagonists, or inhibitors of HTMPN.
Antibodies useful for diagnostic purposes may be prepared in the
same manner as described above for therapeutics. Diagnostic assays
for HTMPN include methods which utilize the antibody and a label to
detect HTMPN in human body fluids or in extracts of cells or
tissues. The antibodies may be used with or without modification,
and may be labeled by covalent or non-covalent attachment of a
reporter molecule. A wide variety of reporter molecules, several of
which are described above, are known in the art and may be
used.
[0185] A variety of protocols for measuring HTMPN, including
ELISAs, RIAs, and FACS, are known in the art and provide a basis
for diagnosing altered or abnormal levels of HTMPN expression.
Normal or standard values for HTMPN expression are established by
combining body fluids or cell extracts taken from normal mammalian
subjects, preferably human, with antibody to HTMPN under conditions
suitable for complex formation. The amount of standard complex
formation may be quantitated by various methods, preferably by
photometric means. Quantities of HTMPN expressed in subject,
control, and disease samples from biopsied tissues are compared
with the standard values. Deviation between standard and subject
values establishes the parameters for diagnosing disease.
[0186] In another embodiment of the invention, the polynucleotides
encoding HTMPN may be used for diagnostic purposes. The
polynucleotides which may be used include oligonucleotide
sequences, complementary RNA and DNA molecules, and PNAs. The
polynucleotides may be used to detect and quantitate gene
expression in biopsied tissues in which expression of HTMPN may be
correlated with disease. The diagnostic assay may be used to
determine absence, presence, and excess expression of HTMPN, and to
monitor regulation of HTMPN levels during therapeutic
intervention.
[0187] In one aspect, hybridization with PCR probes which are
capable of detecting polynucleotide sequences, including genomic
sequences, encoding HTMPN or closely related molecules may be used
to identify nucleic acid sequences which encode HTMPN. The
specificity of the probe, whether it is made from a highly specific
region, e.g., the 5' regulatory region, or from a less specific
region, e.g., a conserved motif, and the stringency of the
hybridization or amplification (maximal, high, intermediate, or
low), will determine whether the probe identifies only naturally
occurring sequences encoding HTMPN, allelic variants, or related
sequences.
[0188] Probes may also be used for the detection of related
sequences, and should preferably have at least 50% sequence
identity to any of the HTMPN encoding sequences. The hybridization
probes of the subject invention may be DNA or RNA and may be
derived from the sequence of SEQ ID NO:80-158 or from genomic
sequences including promoters, enhancers, and introns of the HTMPN
gene.
[0189] Means for producing specific hybridization probes for DNAs
encoding HTMPN include the cloning of polynucleotide sequences
encoding HTMPN or HTMPN derivatives into vectors for the production
of mRNA probes. Such vectors are known in the art, are commercially
available, and may be used to synthesize RNA probes in vitro by
means of the addition of the appropriate RNA polymerases and the
appropriate labeled nucleotides. Hybridization probes may be
labeled by a variety of reporter groups, for example, by
radionuclides such as .sup.32P or .sup.35S, or by enzymatic labels,
such as alkaline phosphatase coupled to the probe via avidin/biotin
coupling systems, and the like.
[0190] Polynucleotide sequences encoding HTMPN may be used for the
diagnosis of disorders associated with expression of HTMPN.
Examples of such disorders include, but are not limited to, an
immune disorder such as acquired immunodeficiency syndrome (AIDS),
Addison's disease, adult respiratory distress syndrome, allergies,
ankylosing spondylitis, amyloidosis, anemia, asthma,
atherosclerosis, autoimmune hemolytic anemia, autoimmune
thyroiditis, autoimmune polyenodocrinopathy-candidiasis-ectodermal
dystrophy (APECED), bronchitis, cholecystitis, contact dermatitis.
Crohn's disease, atopic dermatitis, dermatomyositis, diabetes
mellitus, emphysema, episodic lymphopenia with lymphocytotoxins,
erythroblastosis fetalis, erythema nodosum, atrophic gastritis,
glomerulonephritis, Goodpasture's syndrome, gout, Graves' disease,
Hashimoto's thyroiditis, hypereosinophilia, irritable bowel
syndrome, multiple sclerosis, myasthenia gravis, myocardial or
pericardial inflammation, osteoarthritis, osteoporosis,
pancreatitis, polymyositis, psoriasis, Reiter's syndrome,
rheumatoid arthritis, scleroderma, Sjogren's syndrome, systemic
anaphylaxis, systemic lupus erythematosus, systemic sclerosis,
thrombocytopenic purpura, ulcerative colitis, uveitis, Werner
syndrome, complications of cancer, hemodialysis, and extracorporeal
circulation, viral, bacterial, fungal, parasitic, protozoal, and
helminthic infections, and trauma; a reproductive disorder such as
a disorder of prolactin production; infertility, including tubal
disease, ovulatory defects, and endometriosis; a disruption of the
estrous cycle, a disruption of the menstrual cycle, polycystic
ovary syndrome, ovarian hyperstimulation syndrome, endometrial and
ovarian tumors, uterine fibroids, autoimmune disorders, ectopic
pregnancies, and teratogenesis; cancer of the breast, fibrocystic
breast disease, and galactorrhea; disruptions of spermatogenesis,
abnormal sperm physiology, cancer of the testis, cancer of the
prostate, benign prostatic hyperplasia, prostatitis, Peyronie's
disease, impotence, carcinoma of the male breast, and gynecomastia;
a smooth muscle disorder such as angina, anaphylactic shock,
arrhythmias, asthma, cardiovascular shock, Cushing's syndrome,
hypertension, hypoglycemia, myocardial infarction, migraine, and
pheochromocytoma, and myopathies including cardiomyopathy,
encephalopathy, epilepsy, Kearns-Sayre syndrome, lactic acidosis,
myoclonic disorder, and ophthalmoplegia; a neurological disorder
such as epilepsy, ischemic cerebrovascular disease, stroke,
cerebral neoplasms, Alzheimer's disease, Pick's disease,
Huntington's disease, dementia, Parkinson's disease and other
extrapyramidal disorders, amyotrophic lateral sclerosis and other
motor neuron disorders, progressive neural muscular atrophy,
retinitis pigmentosa, hereditary ataxias, multiple sclerosis and
other demyelinating diseases, bacterial and viral meningitis, brain
abscess, subdural empyema, epidural abscess, suppurative
intracranial thrombophlebitis, myelitis and radiculitis, viral
central nervous system disease; prion diseases including kuru,
Creutzfeldt-Jakob disease, and Gerstmann-Straussler-Scheinker
syndrome; fatal familial insomnia, nutritional and metabolic
diseases of the nervous system, neurofibromatosis, tuberous
sclerosis, cerebelloretinal hemangioblastomatosis,
encephalotrigeminal syndrome, mental retardation and other
developmental disorders of the central nervous system, cerebral
palsy, neuroskeletal disorders, autonomic nervous system disorders,
cranial nerve disorders, spinal cord diseases, muscular dystrophy
and other neuromuscular disorders, peripheral nervous system
disorders, dermatomyositis and polymyositis; inherited, metabolic,
endocrine, and toxic myopathies; myasthenia gravis, periodic
paralysis; mental disorders including mood, anxiety, and
schizophrenic disorders; akathesia, amnesia, catatonia, diabetic
neuropathy, tardive dyskinesia, dystonias, paranoid psychoses,
postherpetic neuralgia, and Tourette's disorder; a gastrointestinal
disorder such as dysphagia, peptic esophagitis, esophageal spasm,
esophageal stricture, esophageal carcinoma, dyspepsia, indigestion,
gastritis, gastric carcinoma, anorexia, nausea, emesis,
gastroparesis, antral or pyloric edema, abdominal angina, pyrosis,
gastroenteritis, intestinal obstruction, infections of the
intestinal tract, peptic ulcer, cholelithiasis, cholecystitis,
cholestasis, pancreatitis, pancreatic carcinoma, biliary tract
disease, hepatoma, infectious colitis, ulcerative colitis,
ulcerative proctitis, Crohn's disease, Whipple's disease,
Mallory-Weiss syndrome, colonic carcinoma, colonic obstruction,
irritable bowel syndrome, short bowel syndrome, diarrhea,
constipation, gastrointestinal hemorrhage, and acquired
immunodeficiency syndrome (AIDS) enteropathy, cirrhosis, jaundice,
cholestasis, hereditary hyperbilirubinemia, hepatic encephalopathy,
hepatorenal syndrome, hepatitis, hepatic steatosis,
hemochromatosis, Wilson's disease, .alpha..sub.1antitrypsin
deficiency, Reye's syndrome, primary sclerosing cholangitis, liver
infarction, portal vein obstruction and thrombosis, passive
congestion, centrilobular necrosis, peliosis hepatis, hepatic vein
thrombosis, veno-occlusive disease, preeclampsia, eclampsia, acute
fatty liver of pregnancy, intrahepatic cholestasis of pregnancy,
and hepatic tumors including nodular hyperplasias, adenomas, and
carcinomas; a cell proliferative disorder such as actinic
keratosis, arteriosclerosis, atherosclerosis, bursitis, cirrhosis,
hepatitis, mixed connective tissue disease (MCTD), myelofibrosis,
paroxysmal nocturnal hemoglobinuria, polycythemia vera, psoriasis,
primary thrombocythemia, and cancers including adenocarcinoma,
leukemia, lymphoma, melanoma, myeloma, sarcoma, teratocarcinoma,
and, in particular, cancers of the adrenal gland, bladder, bone,
bone marrow, brain, breast, cervix, gall bladder, ganglia,
gastrointestinal tract, heart, kidney, liver, lung, muscle, ovary,
pancreas, parathyroid, penis, prostate, salivary glands, skin,
spleen, testis, thymus, thyroid, and uterus; and a developmental
disorder including, but not limited to, those listed above. The
polynucleotide sequences encoding HTMPN may be used in Southern or
northern analysis, dot blot, or other membrane-based technologies;
in PCR technologies; in dipstick, pin, and multiformat ELISA-like
assays; and in microarrays utilizing fluids or tissues from
patients to detect altered HTMPN expression. Such qualitative or
quantitative methods are well known in the art.
[0191] In a particular aspect, the nucleotide sequences encoding
HTMPN may be useful in assays that detect the presence of
associated disorders, particularly those mentioned above. The
nucleotide sequences encoding HTMPN may be labeled by standard
methods and added to a fluid or tissue sample from a patient under
conditions suitable for the formation of hybridization complexes.
After a suitable incubation period, the sample is washed and the
signal is quantitated and compared with a standard value. If the
amount of signal in the patient sample is significantly altered in
comparison to a control sample then the presence of altered levels
of nucleotide sequences encoding HTMPN in the sample indicates the
presence of the associated disorder. Such assays may also be used
to evaluate the efficacy of a particular therapeutic treatment
regimen in animal studies, in clinical trials, or to monitor the
treatment of an individual patient.
[0192] In order to provide a basis for the diagnosis of a disorder
associated with expression of HTMPN, a normal or standard profile
for expression is established. This may be accomplished by
combining body fluids or cell extracts taken from normal subjects,
either animal or human, with a sequence, or a fragment thereof,
encoding HTMPN, under conditions suitable for hybridization or
amplification. Standard hybridization may be quantified by
comparing the values obtained from normal subjects with values from
an experiment in which a known amount of a substantially purified
polynucleotide is used. Standard values obtained in this manner may
be compared with values obtained from samples from patients who are
symptomatic for a disorder. Deviation from standard values is used
to establish the presence of a disorder.
[0193] Once the presence of a disorder is established and a
treatment protocol is initiated, hybridization assays may be
repeated on a regular basis to determine if the level of expression
in the patient begins to approximate that which is observed in the
normal subject. The results obtained from successive assays may be
used to show the efficacy of treatment over a period ranging from
several days to months.
[0194] With respect to cancer, the presence of an abnormal amount
of transcript (either under- or overexpressed) in biopsied tissue
from an individual may indicate a predisposition for the
development of the disease, or may provide a means for detecting
the disease prior to the appearance of actual clinical symptoms. A
more definitive diagnosis of this type may allow health
professionals to employ preventative measures or aggressive
treatment earlier thereby preventing the development or further
progression of the cancer.
[0195] Additional diagnostic uses for oligonucleotides designed
from the sequences encoding HTMPN may involve the use of PCR. These
oligomers may be chemically synthesized, generated enzymatically,
or produced in vitro. Oligomers will preferably contain a fragment
of a polynucleotide encoding HTMPN, or a fragment of a
polynucleotide complementary to the polynucleotide encoding HTMPN,
and will be employed under optimized conditions for identification
of a specific gene or condition. Oligomers may also be employed
under less stringent conditions for detection or quantitation of
closely related DNA or RNA sequences.
[0196] Methods which may also be used to quantitate the expression
of HTMPN include radiolabeling or biotinylating nucleotides,
coamplification of a control nucleic acid, and interpolating
results from standard curves. (See, e.g., Melby, P. C. et al.
(1993) J. Immunol. Methods 159:235-244; Duplaa, C. et al. (1993)
Anal. Biochem. 212:229-236.) The speed of quantitation of multiple
samples may be accelerated by running the assay in an ELISA format
where the oligomer of interest is presented in various dilutions
and a spectrophotometric or colorimetric response gives rapid
quantitation.
[0197] In further embodiments, oligonucleotides or longer fragments
derived from any of the polynucleotide sequences described herein
may be used as targets in a microarray. The microarray can be used
to monitor the expression level of large numbers of genes
simultaneously and to identify genetic variants, mutations, and
polymorphisms. This information may be used to determine gene
function, to understand the genetic basis of a disorder, to
diagnose a disorder, and to develop and monitor the activities of
therapeutic agents.
[0198] Microarrays may be prepared, used, and analyzed using
methods known in the art. (See, e.g., Brennan, T. M. et al. (1995)
U.S. Pat. No. 5,474,796; Schena, M. et al. (1996) Proc. Natl. Acad.
Sci. 93:10614-10619; Baldeschweiler et al. (1995) PCT application
WO95/251116; Shalon, D. et al. (1995) PCT application WO95/35505;
Heller, R. A. et al. (1997) Proc. Natl. Acad. Sci. 94:2150-2155;
and Heller, M. J. et al. (1997) U.S. Pat. No. 5,605,662.)
[0199] In another embodiment of the invention, nucleic acid
sequences encoding HTMPN may be used to generate hybridization
probes useful in mapping the naturally occurring genomic sequence.
The sequences may be mapped to a particular chromosome, to a
specific region of a chromosome, or to artificial chromosome
constructions, e.g., human artificial chromosomes (HACs), yeast
artificial chromosomes (YACs), bacterial artificial chromosomes
(BACs), bacterial P1 constructions, or single chromosome cDNA
libraries. (See, e.g., Harrington, J. J. et al. (1997) Nat. Genet.
15:345-355; Price, C. M. (1993) Blood Rev. 7:127-134; and Trask, B.
J. (1991) Trends Genet. 7:149-154.)
[0200] Fluorescent in situ hybridization (FISH) may be correlated
with other physical chromosome mapping techniques and genetic map
data. (See, e.g., Heinz-Ulrich, et al. (1995) in Meyers, supra, pp.
965-968.) Examples of genetic map data can be found in various
scientific journals or at the Online Mendelian Inheritance in Man
(OMIM) site. Correlation between the location of the gene encoding
HTMPN on a physical chromosomal map and a specific disorder, or a
predisposition to a specific disorder, may help define the region
of DNA associated with that disorder. The nucleotide sequences of
the invention may be used to detect differences in gene sequences
among normal, carrier, and affected individuals.
[0201] In situ hybridization of chromosomal preparations and
physical mapping techniques, such as linkage analysis using
established chromosomal markers, may be used for extending genetic
maps. Often the placement of a gene on the chromosome of another
mammalian species, such as mouse, may reveal associated markers
even if the number or arm of a particular human chromosome is not
known. New sequences can be assigned to chromosomal arms by
physical mapping. This provides valuable information to
investigators searching for disease genes using positional cloning
or other gene discovery techniques. Once the disease or syndrome
has been crudely localized by genetic linkage to a particular
genomic region, e.g., ataxia-telangiectasia to 11q22-23, any
sequences mapping to that area may represent associated or
regulatory genes for further investigation. (See, e.g., Gatti, R.
A. et al. (1988) Nature 336:577-580.) The nucleotide sequence of
the subject invention may also be used to detect differences in the
chromosomal location due to translocation, inversion, etc., among
normal, carrier, or affected individuals.
[0202] In another embodiment of the invention, HTMPN, its catalytic
or immunogenic fragments, or oligopeptides thereof can be used for
screening libraries of compounds in any of a variety of drug
screening techniques. The fragment employed in such screening may
be free in solution, affixed to a solid support, borne on a cell
surface, or located intracellularly. The formation of binding
complexes between HTMPN and the agent being tested may be
measured.
[0203] Another technique for drug screening provides for high
throughput screening of compounds having suitable binding affinity
to the protein of interest. (See, e.g., Geysen, et al. (1984) PCT
application WO84/03564.) In this method, large numbers of different
small test compounds are synthesized on a solid substrate. The test
compounds are reacted with HTMPN, or fragments thereof, and washed.
Bound HTMPN is then detected by methods well known in the art.
Purified HTMPN can also be coated directly onto plates for use in
the aforementioned drug screening techniques. Alternatively,
non-neutralizing antibodies can be used to capture the peptide and
immobilize it on a solid support.
[0204] In another embodiment, one may use competitive drug
screening assays in which neutralizing antibodies capable of
binding HTMPN specifically compete with a test compound for binding
HTMPN. In this manner, antibodies can be used to detect the
presence of any peptide which shares one or more antigenic
determinants with HTMPN.
[0205] In additional embodiments, the nucleotide sequences which
encode HTMPN may be used in any molecular biology techniques that
have yet to be developed, provided the new techniques rely on
properties of nucleotide sequences that are currently known,
including, but not limited to, such properties as the triplet
genetic code and specific base pair interactions.
[0206] Without further elaboration, it is believed that one skilled
in the art can, using the preceding description, utilize the
present invention to its fullest extent. The following preferred
specific embodiments are, therefore, to be construed as merely
illustrative, and not limitative of the remainder of the disclosure
in any way whatsoever.
[0207] The entire disclosure of all applications, patents, and
publications, cited above and below, and of U.S. provisional
applications 60/087,260 (filed May 29, 1998), 60/091,674 (filed
Jul. 2, 1998), 60/102,954 (filed Oct. 2, 1998), and 60/109,869
(filed Nov. 24, 1998) is hereby incorporated by reference.
EXAMPLES
I. Construction of cDNA Libraries
[0208] RNA was purchased from Clontech or isolated from tissues
described in Table 4. Some tissues were homogenized and lysed in
guanidinium isothiocyanate, while others were homogenized and lysed
in phenol or in a suitable mixture of denaturants, such as TRIZOL
(Life Technologies), a monophasic solution of phenol and guanidine
isothiocyanate. The resulting lysates were centrifuged over CsCl
cushions or extracted with chloroform. RNA was precipitated from
the lysates with either isopropanol or sodium acetate and ethanol,
or by other routine methods.
[0209] Phenol extraction and precipitation of RNA were repeated as
necessary to increase RNA purity. In some cases, RNA was treated
with DNase. For most libraries, poly(A+) RNA was isolated using
oligo d(T)-coupled paramagnetic particles (Promega), OLIGOTEX latex
particles (QIAGEN, Valencia Calif.), or an OLIGOTEX mRNA
purification kit (QIAGEN). Alternatively, RNA was isolated directly
from tissue lysates using other RNA isolation kits, e.g., the
POLY(A)PURE mRNA purification kit (Ambion, Austin Tex.).
[0210] In some cases, Stratagene was provided with RNA and
constructed the corresponding cDNA libraries. Otherwise, cDNA was
synthesized and cDNA libraries were constructed with the UNIZAP
vector system (Stratagene) or SUPERSCRIPT plasmid system (Life
Technologies), using the recommended procedures or similar methods
known in the art. (See, e.g., Ausubel, 1997, supra, units 5.1-6.6).
Reverse transcription was initiated using oligo d(T) or random
primers. Synthetic oligonucleotide adapters were ligated to double
stranded cDNA, and the cDNA was digested with the appropriate
restriction enzyme or enzymes. For most libraries, the cDNA was
size-selected (300-1000 bp) using SEPHACRYL S1000, SEPHAROSE CL2B,
or SEPHAROSE CL4B column chromatography (Amersham Pharmacia
Biotech) or preparative agarose gel electrophoresis. cDNAs were
ligated into compatible restriction enzyme sites of the polylinker
of a suitable plasmid, e.g., PBLUESCRIPT plasmid (Stratagene),
pSPORT1 plasmid (Life Technologies), or pINCY (Incyte
Pharmaceuticals, Palo Alto Calif.). Recombinant plasmids were
transformed into competent E. coli cells including XL1-Blue,
XL1-BlueMRF, or SOLR from Stratagene or DH5a, DH10B, or ElectroMAX
DH10B from Life Technologies.
II. Isolation of cDNA Clones
[0211] Plasmids were recovered from host cells by in vivo excision,
using the UNIZAP vector system (Stratagene) or cell lysis. Plasmids
were purified using at least one of the following: a Magic or
WIZARD Minipreps DNA purification system (Promega); an AGTC
Miniprep purification kit (Edge Biosystems, Gaithersburg Md.); and
QIAWELL 8 Plasmid, QIAWELL 8 Plus Plasmid, QIAWELL 8 Ultra Plasmid
purification systems or the REAL Prep 96 plasmid kit from QIAGEN.
Following precipitation, plasmids were resuspended in 0.1 ml of
distilled water and stored, with or without lyophilization, at
4.degree. C.
[0212] Alternatively, plasmid DNA was amplified from host cell
lysates using direct link PCR in a high-throughput format (Rao, V.
B. (1994) Anal. Biochem. 216:1-14). Host cell lysis and thermal
cycling steps were carried out in a single reaction mixture.
Samples were processed and stored in 384-well plates, and the
concentration of amplified plasmid DNA was quantified
fluorometrically using PICOGREEN dye (Molecular Probes, Eugene
Oreg.) and a Fluoroskan II fluorescence scanner (Labsystems Oy,
Helsinki, Finland).
III. Sequencing and Analysis
[0213] The cDNAs were prepared for sequencing using the ABI
CATALYST 800 (Perkin-Elmer) or the HYDRA microdispenser (Robbins
Scientific) or MICROLAB 2200 (Hamilton) systems in combination with
the PTC-200 thermal cyclers (MJ Research). The cDNAs were sequenced
using the ABI PRISM 373 or 377 sequencing systems (Perkin-Elmer)
and standard ABI protocols, base calling software, and kits. In one
alternative, cDNAs were sequenced using the MEGABACE 1000 DNA
sequencing system (Molecular Dynamics). In another alternative, the
cDNAs were amplified and sequenced using the ABI PRISM BIGDYE
Terminator cycle sequencing ready reaction kit (Perkin-Elmer). In
yet another alternative. cDNAs were sequenced using solutions and
dyes from Amersham Pharmacia Biotech. Reading frames for the ESTs
were determined using standard methods (reviewed in Ausubel, 1997,
supra. unit 7.7). Some of the cDNA sequences were selected for
extension using the techniques disclosed in Example V.
[0214] The polynucleotide sequences derived from cDNA, extension,
and shotgun sequencing were assembled and analyzed using a
combination of software programs which utilize algorithms well
known to those skilled in the art. Table 5 summarizes the software
programs, descriptions, references, and threshold parameters used.
The first column of Table 5 shows the tools, programs, and
algorithms used, the second column provides a brief description
thereof, the third column presents the references which are
incorporated by reference herein, and the fourth column presents,
where applicable, the scores, probability values, and other
parameters used to evaluate the strength of a match between two
sequences (the higher the probability the greater the homology).
Sequences were analyzed using MAcDNASIS PRO software (Hitachi
Software Engineering, South San Francisco Calif.) and LASERGENE
software (DNASTAR).
[0215] The polynucleotide sequences were validated by removing
vector, linker, and polyA sequences and by masking ambiguous bases,
using algorithms and programs based on BLAST, dynamic programing,
and dinucleotide nearest neighbor analysis. The sequences were then
queried against a selection of public databases such as GenBank
primate, rodent, mammalian, vertebrate, and eukaryote databases,
and BLOCKS to acquire annotation, using programs based on BLAST,
FASTA, and BLIMPS. The sequences were assembled into full length
polynucleotide sequences using programs based on Phred, Phrap, and
Consed, and were screened for open reading frames using programs
based on GeneMark, BLAST, and FASTA. The full length polynucleotide
sequences were translated to derive the corresponding full length
amino acid sequences, and these full length sequences were
subsequently analyzed by querying against databases such as the
GenBank databases (described above), SwissProt, BLOCKS, PRINTS,
Prosite, and Hidden Markov Model (HMM)-based protein family
databases such as PFAM. HMM is a probalistic approach which
analyzes consensus primary structures of gene families. (See, e.g.,
Eddy, S. R. (1996) Cur. Opin. Str. Biol. 6:361-365.)
[0216] The programs described above for the assembly and analysis
of full length polynucleotide and amino acid sequences were also
used to identify polynucleotide sequence fragments from SEQ ID
NO:80-158. Fragments from about 20 to about 4000 nucleotides which
are useful in hybridization and amplification technologies were
described in The Invention section above.
IV. Northern Analysis
[0217] Northern analysis is a laboratory technique used to detect
the presence of a transcript of a gene and involves the
hybridization of a labeled nucleotide sequence to a membrane on
which RNAs from a particular cell type or tissue have been bound.
(See, e.g., Sambrook, supra, ch. 7; Ausubel, 1995, supra, ch. 4 and
16.)
[0218] Analogous computer techniques applying BLAST were used to
search for identical or related molecules in nucleotide databases
such as GenBank or LIFESEQ database (Incyte Pharmaceuticals). This
analysis is much faster than multiple membrane-based
hybridizations. In addition, the sensitivity of the computer search
can be modified to determine whether any particular match is
categorized as exact or similar. The basis of the search is the
product score, which is defined as:
% sequence identity .times. % maximum BLAST score 100
##EQU00001##
The product score takes into account both the degree of similarity
between two sequences and the length of the sequence match. For
example, with a product score of 40, the match will be exact within
a 1% to 2% error, and, with a product score of 70, the match will
be exact. Similar molecules are usually identified by selecting
those which show product scores between 15 and 40, although lower
scores may identify related molecules.
[0219] The results of northern analyses are reported as a
percentage distribution of libraries in which the transcript
encoding HTMPN occurred. Analysis involved the categorization of
cDNA libraries by organ/tissue and disease. The organ/tissue
categories included cardiovascular, dermatologic, developmental,
endocrine, gastrointestinal, hematopoietic/immune, musculoskeletal,
nervous, reproductive, and urologic. The disease/condition
categories included cancer, inflammation/trauma, cell
proliferation, neurological, and pooled. For each category, the
number of libraries expressing the sequence of interest was counted
and divided by the total number of libraries across all categories.
Percentage values of tissue-specific and disease- or
condition-specific expression are reported in Table 3.
V. Extension of HTMPN Encoding Polynucleotides
[0220] Full length nucleic acid sequences of SEQ ID NOs:80-120 were
produced by extension of the component fragments described in Table
1, column 5, using oligonucleotide primers based on these
fragments. For each nucleic acid sequence, one primer was
synthesized to initiate extension of an antisense polynucleotide,
and the other was synthesized to initiate extension of a sense
polynucleotide. Primers were used to facilitate the extension of
the known sequence "outward" generating amplicons containing new
unknown nucleotide sequence for the region of interest. The initial
primers were designed from the cDNA using OLIGO.TM. 4.06 (National
Biosciences, Plymouth, Minn.), or another appropriate program, to
be about 22 to 30 nucleotides in length, to have a GC content of
about 50% or more, and to anneal to the target sequence at
temperatures of about 68.degree. C. to about 72.degree. C. Any
stretch of nucleotides which would result in hairpin structures and
primer-primer dimerizations was avoided.
[0221] Selected human cDNA libraries (GIBco BRL) were used to
extend the sequence. If more than one extension is necessary or
desired, additional sets of primers are designed to further extend
the known region.
[0222] High fidelity amplification was obtained by following the
instructions for the XL-PCR.TM. kit (The Perkin-Elmer Corp.,
Norwalk, Conn.) and thoroughly mixing the enzyme and reaction mix.
PCR was performed using the PTC-200 thermal cycler (MJ Research,
Inc., Watertown, Mass.), beginning with 40 pmol of each primer and
the recommended concentrations of all other components of the kit,
with the following parameters:
TABLE-US-00001 Step 1 94.degree. C. for 1 min (initial
denaturation) Step 2 65.degree. C. for 1 min Step 3 68.degree. C.
for 6 min Step 4 94.degree. C. for 15 sec Step 5 65.degree. C. for
1 min Step 6 68.degree. C. for 7 min Step 7 Repeat steps 4 through
6 for an additional 15 cycles Step 8 94.degree. C. for 15 sec Step
9 65.degree. C. for 1 min Step 10 68.degree. C. for 7:15 min Step
11 Repeat steps 8 through 10 for an additional 12 cycles Step 12
72.degree. C. for 8 min Step 13 4.degree. C. (and holding)
[0223] A 5 .mu.l to 10 .mu.l aliquot of the reaction mixture was
analyzed by electrophoresis on a low concentration (about 0.6% to
0.8%) agarose mini-gel to determine which reactions were successful
in extending the sequence. Bands thought to contain the largest
products were excised from the gel, purified using QIAQUICK.TM.
(QIAGEN Inc.), and trimmed of overhangs using Klenow enzyme to
facilitate religation and cloning.
[0224] After ethanol precipitation, the products were redissolved
in 13 .mu.l of ligation buffer, 1 .mu.l T4-DNA ligase (15 units)
and 1 T4 polynucleotide kinase were added, and the mixture was
incubated at room temperature for 2 to 3 hours, or overnight at
16.degree. C. Competent E. coli cells (in 40 .mu.l of appropriate
media) were transformed with 3 .mu.l of ligation mixture and
cultured in 80 .mu.l of SOC medium. (See, e.g., Sambrook, supra,
Appendix A, p. 2.) After incubation for one hour at 37.degree. C.,
the E. coli mixture was plated on Luria Bertani (LB) agar (See,
e.g., Sambrook, supra, Appendix A, p. 1) containing carbenicillin
(2.times. carb). The following day, several colonies were randomly
picked from each plate and cultured in 150 .mu.l of liquid
LB/2.times. carb medium placed in an individual well of an
appropriate commercially-available sterile 96-well microtiter
plate. The following day, 5 .mu.l of each overnight culture was
transferred into a non-sterile 96-well plate and, after dilution
1:10 with water, 5 .mu.l from each sample was transferred into a
PCR array.
[0225] For PCR amplification, 18 .mu.l of concentrated PCR reaction
mix (3.3.times.) containing 4 units of rTth DNA polymerase, a
vector primer, and one or both of the gene specific primers used
for the extension reaction were added to each well. Amplification
was performed using the following conditions:
TABLE-US-00002 Step 1 94.degree. C. for 60 sec Step 2 94.degree. C.
for 20 sec Step 3 55.degree. C. for 30 sec Step 4 72.degree. C. for
90 sec Step 5 Repeat steps 2 through 4 for an additional 29 cycles
Step 6 72.degree. C. for 180 sec Step 7 4.degree. C. (and
holding)
[0226] Aliquots of the PCR reactions were run on agarose gels
together with molecular weight markers. The sizes of the PCR
products were compared to the original partial cDNAs, and
appropriate clones were selected, ligated into plasmid, and
sequenced.
[0227] The full length nucleic acid sequences of SEQ ID NO:121-158
were produced by extension of an appropriate fragment of the full
length molecule using oligonucleotide primers designed from this
fragment. One primer was synthesized to initiate 5' extension of
the known fragment, and the other primer, to initiate 3' extension
of the known fragment. The initial primers were designed using
OLIGO 4.06 software (National Biosciences), or another appropriate
program, to be about 22 to 30 nucleotides in length, to have a GC
content of about 50% or more, and to anneal to the target sequence
at temperatures of about 68.degree. C. to about 72.degree. C. Any
stretch of nucleotides which would result in hairpin structures and
primer-primer dimerizations was avoided.
[0228] Selected human cDNA libraries were used to extend the
sequence. If more than one extension was necessary or desired,
additional or nested sets of primers were designed.
[0229] High fidelity amplification was obtained by PCR using
methods well known in the art. PCR was performed in 96-well plates
using the PTC-200 thermal cycler (MJ Research, Inc.). The reaction
mix contained DNA template, 200 nmol of each primer, reaction
buffer containing Mg.sup.2+, (NH.sub.4).sub.2SO.sub.4, and
.beta.-mercaptoethanol, Taq DNA polymerase (Amersham Pharmacia
Biotech), ELONGASE enzyme (Life Technologies), and Pfu DNA
polymerase (Stratagene), with the following parameters for primer
pair PCI A and PCI B: Step 1: 94.degree. C., 3 min: Step 2:
94.degree. C., 15 sec; Step 3: 60.degree. C., 1 min; Step 4:
68.degree. C., 2 min; Step 5: Steps 2, 3, and 4 repeated 20 times;
Step 6: 68.degree. C., 5 min; Step 7: storage at 4.degree. C. In
the alternative, the parameters for primer pair T7 and SK+ were as
follows: Step 1: 94.degree. C., 3 min; Step 2: 94.degree. C., 15
sec; Step 3: 57.degree. C., 1 min; Step 4: 68.degree. C., 2 min;
Step 5: Steps 2, 3, and 4 repeated 20 times; Step 6: 68.degree. C.,
5 min; Step 7: storage at 4.degree. C.
[0230] The concentration of DNA in each well was determined by
dispensing 1000 PICOGREEN quantitation reagent (0.25% (v/v)
PICOGREEN; Molecular Probes, Eugene Oreg.) dissolved in 1.times.TE
and 0.5 .mu.l of undiluted PCR product into each well of an opaque
fluorimeter plate (Corning Costar, Acton Mass.), allowing the DNA
to bind to the reagent. The plate was scanned in a Fluoroskan II
(Labsystems Oy, Helsinki, Finland) to measure the fluorescence of
the sample and to quantify the concentration of DNA. A 5 .mu.l to
10 .mu.l aliquot of the reaction mixture was analyzed by
electrophoresis on a 1% agarose mini-gel to determine which
reactions were successful in extending the sequence.
[0231] The extended nucleotides were desalted and concentrated,
transferred to 384-well plates, digested with CviJI cholera virus
endonuclease (Molecular Biology Research, Madison Wis.), and
sonicated or sheared prior to religation into pUC 18 vector
(Amersham Pharmacia Biotech). For shotgun sequencing, the digested
nucleotides were separated on low concentration (0.6 to 0.8%)
agarose gels, fragments were excised, and agar digested with Agar
ACE (Promega). Extended clones were religated using T4 ligase (New
England Biolabs, Beverly Mass.) into pUC 18 vector (Amersham
Pharmacia Biotech), treated with Pfu DNA polymerase (Stratagene) to
fill-in restriction site overhangs, and transfected into competent
E. coli cells. Transformed cells were selected on
antibiotic-containing media, individual colonies were picked and
cultured overnight at 37.degree. C. in 384-well plates in
LB/2.times. carb liquid media.
[0232] The cells were lysed, and DNA was amplified by PCR using Taq
DNA polymerase (Amersham Pharmacia Biotech) and Pfu DNA polymerase
(Stratagene) with the following parameters: Step 1: 94.degree. C.,
3 min; Step 2: 94.degree. C., 15 sec; Step 3: 60.degree. C., 1 min;
Step 4: 72.degree. C., 2 min; Step 5: steps 2, 3, and 4 repeated 29
times; Step 6: 72.degree. C., 5 min; Step 7: storage at 4.degree.
C. DNA was quantified by PICOGREEN reagent (Molecular Probes) as
described above. Samples with low DNA recoveries were reamplified
using the same conditions as described above. Samples were diluted
with 20% dimethysulphoxide (1:2, v/v), and sequenced using DYENAMIC
energy transfer sequencing primers and the DYENAMIC DIRECT kit
(Amersham Pharmacia Biotech) or the ABI PRISM BIGDYE Terminator
cycle sequencing ready reaction kit (Perkin-Elmer).
[0233] In like manner, the nucleotide sequences of SEQ ID NO:80-158
are used to obtain 5' regulatory sequences using the procedure
above, oligonucleotides designed for such extension, and an
appropriate genomic library.
VI. Labeling and Use of Individual Hybridization Probes
[0234] Hybridization probes derived from SEQ ID NO:80-158 are
employed to screen cDNAs, genomic DNAs, or mRNAs. Although the
labeling of oligonucleotides, consisting of about 20 base pairs, is
specifically described, essentially the same procedure is used with
larger nucleotide fragments. Oligonucleotides are designed using
state-of-the-art software such as OLIGO 4.06 software (National
Biosciences) and labeled by combining 50 pmol of each oligomer, 250
.mu.Ci of [.gamma.-.sup.32P] adenosine triphosphate (Amersham
Pharmacia Biotech), and T4 polynucleotide kinase (DuPont NEN,
Boston Mass.). The labeled oligonucleotides are substantially
purified using a SEPHADEX G-25 superfine size exclusion dextran
bead column (Amersham Pharmacia Biotech). An aliquot containing
10.sup.7 counts per minute of the labeled probe is used in a
typical membrane-based hybridization analysis of human genomic DNA
digested with one of the following endonucleases: Ase I, Bgl II,
Eco RI, Pst I, XbaI, or Pvu II (DuPont NEN).
[0235] The DNA from each digest is fractionated on a 0.7% agarose
gel and transferred to nylon membranes (Nytran Plus, Schleicher
& Schuell, Durham N.H.). Hybridization is carried out for 16
hours at 40.degree. C. To remove nonspecific signals, blots are
sequentially washed at room temperature under increasingly
stringent conditions up to 0.1.times. saline sodium citrate and
0.5% sodium dodecyl sulfate. After XOMAT-AR film (Eastman Kodak,
Rochester N.Y.) is exposed to the blots to film for several hours,
hybridization patterns are compared visually.
VII. Microarrays
[0236] A chemical coupling procedure and an ink jet device can be
used to synthesize array elements on the surface of a substrate.
(See, e.g., Baldeschweiler, supra.) An array analogous to a dot or
slot blot may also be used to arrange and link elements to the
surface of a substrate using thermal, UV, chemical, or mechanical
bonding procedures. A typical array may be produced by hand or
using available methods and machines and contain any appropriate
number of elements. After hybridization, nonhybridized probes are
removed and a scanner used to determine the levels and patterns of
fluorescence. The degree of complementarity and the relative
abundance of each probe which hybridizes to an element on the
microarray may be assessed through analysis of the scanned
images.
[0237] Full-length cDNAs, Expressed Sequence Tags (ESTs), or
fragments thereof may comprise the elements of the microarray.
Fragments suitable for hybridization can be selected using software
well known in the art such as LASERGENE software (DNASTAR).
Full-length cDNAs, ESTs, or fragments thereof corresponding to one
of the nucleotide sequences of the present invention, or selected
at random from a cDNA library relevant to the present invention,
are arranged on an appropriate substrate, e.g., a glass slide. The
cDNA is fixed to the slide using, e.g., UV cross-linking followed
by thermal and chemical treatments and subsequent drying. (See,
e.g., Schena. M. et al. (1995) Science 270:467-470; Shalon, D. et
al. (1996) Genome Res. 6:639-645.) Fluorescent probes are prepared
and used for hybridization to the elements on the substrate. The
substrate is analyzed by procedures described above.
VIII. Complementary Polynucleotides
[0238] Sequences complementary to the HTMPN-encoding sequences, or
any parts thereof, are used to detect, decrease, or inhibit
expression of naturally occurring HTMPN. Although use of
oligonucleotides comprising from about 15 to 30 base pairs is
described, essentially the same procedure is used with smaller or
with larger sequence fragments. Appropriate oligonucleotides are
designed using OLIGO 4.06 software (National Biosciences) and the
coding sequence of HTMPN. To inhibit transcription, a complementary
oligonucleotide is designed from the most unique 5' sequence and
used to prevent promoter binding to the coding sequence. To inhibit
translation, a complementary oligonucleotide is designed to prevent
ribosomal binding to the HTMPN-encoding transcript.
IX. Expression of HTMPN
[0239] Expression and purification of HTMPN is achieved using
bacterial or virus-based expression systems. For expression of
HTMPN in bacteria, cDNA is subcloned into an appropriate vector
containing an antibiotic resistance gene and an inducible promoter
that directs high levels of cDNA transcription. Examples of such
promoters include, but are not limited to, the trp-lac (tac) hybrid
promoter and the T5 or T7 bacteriophage promoter in conjunction
with the lac operator regulatory element. Recombinant vectors are
transformed into suitable bacterial hosts, e.g., BL21(DE3).
Antibiotic resistant bacteria express HTMPN upon induction with
isopropyl beta-D-thiogalactopyranoside (IPTG). Expression of HTMPN
in eukaryotic cells is achieved by infecting insect or mammalian
cell lines with recombinant Autographica californica nuclear
polyhedrosis virus (AcMNPV), commonly known as baculovirus. The
nonessential polyhedrin gene of baculovirus is replaced with cDNA
encoding HTMPN by either homologous recombination or
bacterial-mediated transposition involving transfer plasmid
intermediates. Viral infectivity is maintained and the strong
polyhedrin promoter drives high levels of cDNA transcription.
Recombinant baculovirus is used to infect Spodoptera frugiperda
(Sf9) insect cells in most cases, or human hepatocytes, in some
cases. Infection of the latter requires additional genetic
modifications to baculovirus. (See Engelhard, E. K. et al. (1994)
Proc. Natl. Acad. Sci. USA 91:3224-3227; Sandig, V. et al. (1996)
Hum. Gene Ther. 7:1937-1945.)
[0240] In most expression systems, HTMPN is synthesized as a fusion
protein with, e.g., glutathione S-transferase (GST) or a peptide
epitope tag, such as FLAG or 6-His, permitting rapid, single-step,
affinity-based purification of recombinant fusion protein from
crude cell lysates. GST, a 26-kilodalton enzyme from Schistosoma
japonicum, enables the purification of fusion proteins on
immobilized glutathione under conditions that maintain protein
activity and antigenicity (Amersham Pharmacia Biotech). Following
purification, the GST moiety can be proteolytically cleaved from
HTMPN at specifically engineered sites. FLAG, an 8-amino acid
peptide, enables immunoaffinity purification using commercially
available monoclonal and polyclonal anti-FLAG antibodies (Eastman
Kodak). 6-His, a stretch of six consecutive histidine residues,
enables purification on metal-chelate resins (QIAGEN). Methods for
protein expression and purification are discussed in Ausubel (1995,
supra, ch 10 and 16). Purified HTMPN obtained by these methods can
be used directly in the following activity assay.
X. Demonstration of HTMPN Activity
[0241] Given the chemical and structural similarity between the
HTMPN and other members of the transmembrane protein families,
HTMPN is identified as a new member of the membrane spanning
proteins and is presumed to be involved in the regulation of cell
growth. To demonstrate that increased levels of HTMPN expression
correlates with decreased cell motility and increased cell
proliferation, expression vectors encoding HTMPN are electroporated
into highly motile cell lines, such as U-937 (ATCC CRL 1593), HEL
92.1.7 (ATCC TIB 180) and MAC10, and the motility of the
electroporated and control cells are compared. Methods for the
design and construction of an expression vector capable of
expressing HTMPN in the desired mammalian cell line(s) chosen are
well known to the art. Assays for examining the motility of cells
in culture are known to the art (cf Miyake, M. et al. (1991) J.
Exp. Med. 174:1347-1354 and Ikeyama, S. et al. (1993) J. Exp. Med.
177:1231-1237). Increasing the level of HTMPN in highly motile cell
lines by transfection with an HTMPN expression vector inhibits or
reduces the motility of these cell lines, and the amount of this
inhibition is proportional to the activity of HTMPN in the
assay.
[0242] Alternatively, the activity of HTMPN may be measured using
an assay based upon the property of MPs to support in vitro
proliferation of fibroblasts and tumor cells under serum-free
conditions. (Chiquet-Ehrismann, R. et al. (1986) Cell 47:131-139.)
Wells in 96 well cluster plates (Falcon, Fisher Scientific, Santa
Clara, Calif.) are coated with HTMPN by incubation with solutions
at 50-100 .mu.g HTMPN/ml for 15 min at ambient temperature. The
coating solution is aspirated, and the wells washed with Dulbecco's
medium before cells are plated. Rat fibroblast cultures or rat
mammary tumor cells are prepared as described. (Chiquet-Ehrismann,
R. et al. supra.) and plated at a density of 10.sup.4-10.sup.5
cells/ml in Dulbecco's medium supplemented with 10% fetal calf
serum.
[0243] After three days the medium is removed, and the cells washed
three times with phosphate-buffered saline (PBS), pH 7.0, before
addition of serum-free Dulbecco's medium containing 0.25 mg/ml
bovine serum albumin (BSA, Fraction V, Sigma Chemical Company, St.
Louis, Mo.). After 2 days the medium is aspirated, and 100 .mu.l of
[.sup.3H]thymidine (NEN) at 2 .mu.Ci/ml in fresh Dulbecco's medium
containing 0.25 mg/ml BSA is added. Parallel plates are fixed and
stained to determine cell numbers. After 16 hr, the medium is
aspirated, the cell layer washed with PBS, and the 10%
trichloroacetic acid-precipitable radioactivity in the cell layer
determined by liquid scintillation counting (normalized to relative
cell numbers; Chiquet-Ehrismann, R. et al. supra). The amount of
radioisotope-labeled DNA incorporated into chromatin under
serum-free conditions is proportional to the activity of HTMPN.
[0244] Alternatively, HTMPN, or biologically active fragments
thereof, are labeled with .sup.125I Bolton-Hunter reagent (See,
e.g., Bolton et al. (1973) Biochem. J. 133:529). Candidate
molecules previously arrayed in the wells of a multi-well plate are
incubated with the labeled HTMPN, washed, and any wells with
labeled HTMPN complex are assayed. Data obtained using different
concentrations of HTMPN are used to calculate values for the
number, affinity, and association of HTMPN with the candidate
molecules.
XI. Functional Assays
[0245] HTMPN function is assessed by expressing the sequences
encoding HTMPN at physiologically elevated levels in mammalian cell
culture systems. cDNA is subcloned into a mammalian expression
vector containing a strong promoter that drives high levels of cDNA
expression. Vectors of choice include pCMV SPORT (Life
Technologies) and pCR3.1 (Invitrogen, Carlsbad Calif.), both of
which contain the cytomegalovirus promoter. 5-10 .mu.g of
recombinant vector are transiently transfected into a human cell
line, preferably of endothelial or hematopoietic origin, using
either liposome formulations or electroporation. 1-2 .mu.g of an
additional plasmid containing sequences encoding a marker protein
are co-transfected. Expression of a marker protein provides a means
to distinguish transfected cells from nontransfected cells and is a
reliable predictor of cDNA expression from the recombinant vector.
Marker proteins of choice include, e.g., Green Fluorescent Protein
(GFP; Clontech), CD64, or a CD64-GFP fusion protein. Flow cytometry
(FCM), an automated, laser optics-based technique, is used to
identify transfected cells expressing GFP or CD64-GFP, and to
evaluate properties, for example, their apoptotic state. FCM
detects and quantifies the uptake of fluorescent molecules that
diagnose events preceding or coincident with cell death. These
events include changes in nuclear DNA content as measured by
staining of DNA with propidium iodide; changes in cell size and
granularity as measured by forward light scatter and 90 degree side
light scatter; down-regulation of DNA synthesis as measured by
decrease in bromodeoxyuridine uptake; alterations in expression of
cell surface and intracellular proteins as measured by reactivity
with specific antibodies; and alterations in plasma membrane
composition as measured by the binding of fluorescein-conjugated
Annexin V protein to the cell surface. Methods in flow cytometry
are discussed in Ormerod, M. G. (1994) Flow Cytometry, Oxford, New
York N.Y.
[0246] The influence of HTMPN on gene expression can be assessed
using highly purified populations of cells transfected with
sequences encoding HTMPN and either CD64 or CD64-GFP. CD64 and
CD64-GFP are expressed on the surface of transfected cells and bind
to conserved regions of human immunoglobulin G (IgG). Transfected
cells are efficiently separated from nontransfected cells using
magnetic beads coated with either human IgG or antibody against
CD64 (DYNAL, Lake Success N.Y.). mRNA can be purified from the
cells using methods well known by those of skill in the art.
Expression of mRNA encoding HTMPN and other genes of interest can
be analyzed by northern analysis or microarray techniques.
XII. Production of HTMPN Specific Antibodies
[0247] HTMPN substantially purified using polyacrylamide gel
electrophoresis (PAGE; see, e.g., Harrington, M. G. (1990) Methods
Enzymol. 182:488-495), or other purification techniques, is used to
immunize rabbits and to produce antibodies using standard
protocols.
[0248] Alternatively, the HTMPN amino acid sequence is analyzed
using LASERGENE software (DNASTAR) to determine regions of high
immunogenicity, and a corresponding oligopeptide is synthesized and
used to raise antibodies by means known to those of skill in the
art. Methods for selection of appropriate epitopes, such as those
near the C-terminus or in hydrophilic regions are well described in
the art. (See, e.g., Ausubel, 1995, supra, ch. 11.)
[0249] Typically, oligopeptides 15 residues in length are
synthesized using an ABI 431A Peptide Synthesizer (Perkin-Elmer)
using fmoc-chemistry and coupled to KLH (Sigma-Aldrich, St. Louis
Mo.) by reaction with N-maleimidobenzoyl-N-hydroxysuccinimide ester
(MBS) to increase immunogenicity. (See, e.g., Ausubel, 1995,
supra.) Rabbits are immunized with the oligopeptide-KLH complex in
complete Freund's adjuvant. Resulting antisera are tested for
antipeptide activity by, for example, binding the peptide to
plastic, blocking with 1% BSA, reacting with rabbit antisera,
washing, and reacting with radio-iodinated goat anti-rabbit
IgG.
XIII. Purification of Naturally Occurring HTMPN Using Specific
Antibodies
[0250] Naturally occurring or recombinant HTMPN is substantially
purified by immunoaffinity chromatography using antibodies specific
for HTMPN. An immunoaffinity column is constructed by covalently
coupling anti-HTMPN antibody to an activated chromatographic resin,
such as CNBr-activated SEPHAROSE (Amersham Pharmacia Biotech).
After the coupling, the resin is blocked and washed according to
the manufacturer's instructions.
[0251] Media containing HTMPN are passed over the immunoaffinity
column, and the column is washed under conditions that allow the
preferential absorbance of HTMPN (e.g., high ionic strength buffers
in the presence of detergent). The column is eluted under
conditions that disrupt antibody/HTMPN binding (e.g., a buffer of
pH 2 to pH 3, or a high concentration of a chaotrope, such as urea
or thiocyanate ion), and HTMPN is collected.
XIV. Identification of Molecules Which Interact with HTMPN
[0252] HTMPN, or biologically active fragments thereof, are labeled
with .sup.125I Bolton-Hunter reagent (See, e.g., Bolton et al.
(1973) Biochem. J. 133:529). Candidate molecules previously arrayed
in the wells of a multi-well plate are incubated with the labeled
HTMPN, washed, and any wells with labeled HTMPN complex are
assayed. Data obtained using different concentrations of HTMPN are
used to calculate values for the number, affinity, and association
of HTMPN with the candidate molecules.
[0253] Various modifications and variations of the described
methods and systems of the invention will be apparent to those
skilled in the art without departing from the scope and spirit of
the invention. Although the invention has been described in
connection with specific preferred embodiments, it should be
understood that the invention as claimed should not be unduly
limited to such specific embodiments. Indeed, various modifications
of the described modes for carrying out the invention which are
obvious to those skilled in molecular biology or related fields are
intended to be within the scope of the following claims.
TABLE-US-00003 TABLE 1 Nucleo- Protein tide SEQ SEQ ID NO: ID NO:
Clone ID Library Fragments 1 80 153831 THPIPLB02 153831
(THPIPLB02), 2700741H1 (OVARTUT10), 881348R1 (THYRNOT02), 1856588F6
(PROSNOT18) 2 81 350629 LVENNOT01 350629 and 350629T6 (LVENNOT01),
3499109H1 (PROSTUT13) 3 82 729171 LUNGNOT03 729171 and 729171R6
(LUNGNOT03), 1645343H1 (HEARFET01), 680519X2 and 680519X1
(UTRSNOT02), 625051R6 (PGANNOT01), 1459466F1 (COLNFET02), 1225759T1
(COLNNOT01), 2590526H1 (LUNGNOT22), 2807811H1 (BLADTUT08) 4 83
1273641 TESTTUT02 1273641 and 1273641F6 (TESTTUT02), 1308181F6 and
1308181F1 (COLNFET02), 1427606F1 (SINTBST01), 756171H1 (BRAITUT02),
2416518F6 (HNT3AZT01), 4242346H1 (SYNWDIT01) 5 84 1427389 SINTBST01
1427389 (SINTBST01), 3097151H1 (CERVNOT03), 723779R1 (SYNOOAT01) 6
85 1458357 COLNFET02 1458357 (COLNFET02), SAOA01955F1, SAOA03146F1,
SAOA03356F1, SAOA00213F1 7 86 1482837 CORPNOT02 1482837 and
1482837T6 (CORPNOT02), 869453H1 (LUNGAST01), 3564972F6 (SKINNOT05),
663983H1 (SCORNOT01), 1315073F6 (BLADTUT02), 3809242H1 (CONTTUT01),
311459T6 (LUNGNOT02), 1798893F6 (COLNNOT27) 8 87 1517434 PANCTUT01
1517434 (PANCTUT01), 2848842H1 (BRSTTUT13), 586843X1 (UTRSNOT01),
1261245R1 (SYNORAT05), 1554505F1 (BLADTUT04) 9 88 1536052 SPLNNOT04
1536052 and 1531447T6 (SPLNNOT04), 1729124T6 (BRSTTUT08) 10 89
1666118 BRSTNOT09 1666118 (BRSTNOT09), 907075R2 (COLNNOT08),
1524914T1 (UCMCL5T01), 1283459F6 (COLNNOT16) 11 90 1675560
BLADNOT05 1675560 and 1675560T6 (BLADNOT05) 12 91 1687323 PROSTUT10
1687323 and 1687323F6(PROSTUT10), 2292356R3 (BRAINON01) 13 92
1692236 PROSTUT10 1692236 (PROSTUT10), 2786557F6 (BRSTNOT13),
602869R6 and 602869T6 (BRSTTUT01), 2258230H1 (OVARTUT01), 780083T1
(MYOMNOT01), 2057230T6 (BEPINOT01), 288105R1 (EOSIHET02) 14 93
1720847 BLADNOT06 1720847, 1722250F6, and 1722250T6 (BLADNOT06) 15
94 1752821 LIVRTUT01 1752821 (LIVRTUT01), 3180328H1 (TLYJNOT01),
1969457T6 (BRSTNOT04), 2608504H1 (BONTNOT01), 2455688T6 and
2455688F6 (ENDANOT01), 1816354F6 (PROSNOT20) 16 95 1810923
PROSTUT12 1810923 and 1810923T6 (PROSTUT12), 3221260H1 (COLNNON03)
17 96 1822315 GBLATUT01 1822315 (GBLATUT01), 1841726H1 (COLNNOT07),
1598582T6 (BLADNOT03), 1264125R1 (SYNORAT05), 645048H1 (BRSTTUT02),
1474782H1 (LUNGTUT03), 352739F1 (LVENNOT01), 876001R1 (LUNGAST01)
18 97 1877777 LEUKNOT03 1877777 (LEUKNOT03), 1219656H1 (NEUTGMT01),
1471553T1 (LUNGTUT03) 19 98 1879819 LEUKNOT03 1879819 (LEUKNOT03),
1734538H1 (COLNNOT22), 1428615F6 (SINTBST01), 3558710H1
(LUNGNOT31), 1996096R6 (BRSTTUT03) 20 99 1932945 COLNNOT16 1932945
(COLNNOT16), 2383333H1 (ISLTNOT01), 2706050F6 (PONSAZT01), 21 100
2061026 OVARNOT03 2061026 (OVARNOT03) 22 101 2096687 BRAITUT02
2096687 (BRAITUT02), 2204640H1 (SPLNFET02) 23 102 2100530 BRAITUT02
2100530 (BRAITUT02), 2740969F6 (BRSTTUT14) 24 103 2357636 LUNGNOT20
2357636 (LUNGNOT20), 2693537H1 (LUNGNOT23), 1794235T6 (PROSTUT05),
235425R6 (SINTNOT02), 760091R1 (BRAITUT02), 887877R1 (PANCNOT05) 25
104 2365230 ADRENOT07 2365230 (ADRENOT07), 2921195H1 (SININOT04) 26
105 2455121 ENDANOT01 2455121 and 2455121F6 (ENDANOT01) 27 106
2472514 THPINOT03 2472514 (THPINOT03), 3212904H1 (BLADNOT08) 28 107
2543486 UTRSNOT11 2543486 (UTRSNOT11), 2374764H1 (ISLTNOT01),
1359576F1 (LUNGNOT12), 1357170H1 (LUNGNOT09) 29 108 2778171
OVARTUT03 2778171 (OVARTUT03), 1822045H1 (GBLATUT01), 1692535F6
(COLNNOT23), 1905275F6 (OVARNOT07) 30 109 2799575 PENCNOT01 2799575
(PENCNOT01), 874115H1 (LUNGAST01), 967837R1 (BRSTNOT05), 3235248T6
and 3235248F6 (COLNUCT03) 31 110 2804955 BLADTUT08 2804955
(BLADTUT08), 732534H1 (LUNGNOT03), 402168R1 (TMLR3DT01), 3481814H1
(KIDNNOT31), 1485989F1 (CORPNOT02) 32 111 2806395 BLADTUT08 2806395
(BLADTUT08), 1579109H1 (DUODNOT01), 1533572F1 (SPLNNOT04),
1889837F6 and 1889837T6 (BLADTUT07), 2414178F6 (HNT3AZT01) 33 112
2836858 TLYMNOT03 2836858 and 2836858CT1 (TLYMNOT03), 2127516H1
(KIDNNOT05) 34 113 2844513 DRGLNOT01 2844513 and 2844513T6
(DRGLNOT01), 388885T6 (THYMNOT02), 287344F1 (EOSIHET02), 3867626H1
(BMARNOT03) 35 114 3000380 TLYMNOT06 3000380 (TLYMNOT06), 1930658H1
(COLNTUT03), 2395295F6 (THPIAZT01), 1242456R6 (LUNGNOT03) 36 115
182532 PLACNOB01 062374H1, 062962R6, 064457R6, and 182532H1
(PLACNOB01), 3144248X12F1 (HNT2AZS07) 37 116 239589 HIPONOT01
239589H1 and 239589X13 (HIPONOT01), 264805R6 (HNT2AGT01), 552683X17
(SCORNOT01), 1595053F1 (BRAINOT14) 38 117 1671302 BMARNOT03
399804H1 (PITUNOT02), 1458549H1 (COLNFET02), 1671302F6 and
1671302H1 (BMARNOT03), 2093453R6 (PANCNOT04), 2498385F6 and
2498385T6 (ADRETUT05) 39 118 2041858 HIPONON02 063184R1
(PLACNOB01), 1294823F1 (PGANNOT03), 1303974F1 (PLACNOT02),
1648770F6 (PROSTUT09), 2041858H1 (HIPONON02) 40 119 2198863
SPLNFET02 1880470F6 (LEUKNOT03), 1888946F6 (BLADTUT07), 2198863F6
and 2198863H1 (SPLNFET02) 41 120 3250703 SEMVNOT03 1317728H1,
1318433H1, 1319354H1, 1319380F1, 1320494H1, and 1320812F1
(BLADNOT04), 3247874H1, 3249188H1, 3249385H1, and 3250703H1
(SEMVNOT03) 42 121 350287 LVENNOT01 062018F1 (PLACNOB01), 350287H1
(LVENNOT01), 869320R1 (LUNGAST01), 1416927F6 (BRAINOT12), 3083789H1
(OVARTUN01) 43 122 1618171 BRAITUT12 1618171F6 and 1618171H1
(BRAITUT12), 3316315F6 (PROSBPT03) 44 123 1625863 COLNPOT01
1625863H1 and 1625863T6 (COLNPOT01), 2100364R6 (BRAITUT02) 45 124
1638353 UTRSNOT06 1638353H1 (UTRSNOT06), 3733085H1 (SMCCNOS01),
3882774T6 (SPLNNOT11), 1626195T6 (COLNPOT01), 1495745H1 (PROSNON01)
46 125 1726843 PROSNOT14 826000T1 (PROSNOT06), 1726843F6 and
1726843H1 (PROSNOT14), 2225762F6 (SEMVNOT01), 2480248H1
(SMCANOT01), 2600692F6 (UTRSNOT10), 2728257F6 (OVARTUT05) 47 126
1754506 LIVRTUT01 907854R2 (COLNNOT09), 1354345F1 (LUNGNOT09),
1359472F1 (LUNGNOT12), 1397284F1 (BRAITUT08), 1557921F1
(BLADTUT04), 1754506F6 and 1754506H1 (LIVRTUT01) 48 127 1831378
THPIAZT01 441541R1 (MPHGNOT03), 712292R6 (SYNORAT04), 1311835F1
(COLNFET02), 1555765F6 (BLADTUT04), 1831378H1 (THPIAZT01),
1865502F6 (PROSNOT19), 3077521H1 (BONEUNT01), 3555043H1
(SYNONOT01), 3774618H1 (BRSTNOT25) 49 128 1864943 PROSNOT19
714070F1 (PROSTUT01), 736327R1 (TONSNOT01), 1864943H1 (PROSNOT19),
2672921F6 (KIDNNOT19) 50 129 1911316 CONNTUT01 777070F1
(COLNNOT05), 1911316H1 and 1911316T6 (CONNTUT01) 51 130 1943120
HIPONOT01 1516263F1 (PANCTUT01), 1943120H1 (HIPONOT01), 2469009F6
(THYRNOT08), 2522459F6 (BRAITUT21), 3202972F6 (PENCNOT02),
4383679H1 (BRAVUTT02) 52 131 2314236 NGANNOT01 2314236H1
(NGANNOT01), 2812085F6 (OVARNOT10), 3949704T6 (DRGCNOT01) 53 132
2479409 SMCANOT01 2479409F6 and 2479409H1 (SMCANOT01) 54 133
2683149 S1N1UCT01 760389H1 (BRAITUT02), 1634372F6 (COLNNOT19),
1695052F6 (COLNNOT23), 1736429F6 (COLNNOT22), 2048429F6
(LIVRFET02), 2683149H1 (SINIUCT01), 3282234F6 (STOMFET02) 55 134
2774051 PANCNOT15 1852505F6 (LUNGFET03), 2774051F6 and 2774051H1
(PANCNOT15) 56 135 2869038 THYRNOT10 536017R6 (ADRENOT03),
2770632F6 (COLANOT02), 2795420F6 (NPOLNOT01), 2869038F6 and
2869038H1 (THYRNOT10), 3323992H1 (PTHYNOT03) 57 136 2918334
THYMFET03 2918334H1 (THYMFET03), SBNA01788F1 58 137 2949916
KIDNFET01 2949916H1 (KIDNFET01), SBMA00738F1 59 138 2989375
KIDNFET02 437481R6 and 437481T6 (THYRNOT01), 2989375H1 (KIDNFET02)
60 139 3316764 PROSBPT03 1328462F1 (PANCNOT07), 1691807F6
(PROSTUT10), 1851237F6 (LUNGFET03), 3316764H1 (PROSBPT03),
5092348H1 (UTRSTMR01) 61 140 3359559 PROSTUT16 943684 and 943564
(ADRENOT03), 1697079F6 (COLNNOT23), 2717735H1 (THYRNOT09),
2792705H1 (COLNTUT16), 3359559H1 (PROSTUT16) 62 141 4289208
BRABDIR01 3990421R6 (LUNGNON03), 4289208H1 (BRABDIR01) 63 142
2454013 ENDANOT01 014571R1 (THP1PLB01), 1303790T1 (PLACNOT02),
1342791T1 (COLNTUT03), 1351680F1 (LATRTUT02), 1359607T1
(LUNGNOT12), 2454013F6 and 2454013H1 (ENDANOT01) 64 143 2454048
ENDANOT01 551329R1 and 2056675R6 (BEPINOT01), 819281R1 (KERANOT02),
2454048H1 (ENDANOT01), 3143588H1 (HNT2AZS07) 65 144 2479282
SMCANOT01 873307R1 (LUNGAST01), 2479282H1 and 2479282T6
(SMCANOT01), 2610082F6 (COLNTUT15), SANA03636F1 66 145 2483432
SMCANOT01 940455T1 (ADRENOT03), 1863558T6 (PROSNOT19), 2483432H1
(SMCANOT01), 2641345H1 (LUNGTUT08), 3245089T6 (BRAINOT19),
SBCA02765F1 67 146 2493824 ADRETUT05 489685F1 (HNT2AGT01), 530794H1
(BRAINOT03), 735826R1 (TONSNOT01), 2056809R6 (BEPINOT01), 2493824H1
(ADRETUT05), 2763162F6 (BRSTNOT12), 2812426H1 (OVARNOT10) 68 147
2555823 THYMNOT03 1266972F6 (BRAINOT09), 1335461T1 (COLNNOT13),
1900947F6 (BLADTUT06), 1942256T6 (HIPONOT01), 2555823H1
(THYMNOT03), SARB01019F1, SARB01303F1 69 148 2598242 OVARTUT02
320268F1 (EOSIHET02), 738915R1 (PANCNOT04), 1250161F1 (LUNGFET03),
2598242F6 and 2598242H1 (OVARTUT02), 5020793H1 (OVARNON03),
SASA00178F1 70 149 2634120 COLNTUT15 1398694F1 (BRAITUT08),
1506594F1 (BRAITUT07), 2120954F6 (BRSTNOT07), 2634120F6 and
2634120H1 (COLNTUT15), 2761586H1 (BRAINOS12), 2806841F6 (BLADTUT08)
71 150 2765411 BRSTNOT12 2765236T6 and 27654HH1 (BRSTNOT12),
4058218H1 (SPLNNOT13) 72 151 2769412 COLANOT02 1715480F6
(UCMCNOT02), 2769412H1 (COLANOT02), SBDA04076F1 73 152 2842779
DRGLNOT01 12627HR1 (SYNORAT05), 1710449T6 (PROSNOT16), 2842779F6
(DRGLNOT01), 2842779H1 (DRGLNOT01), 2850941F6 (BRSTTUT13),
3123378H1 (LNODNOT05), 3457873H1 (293TF1T01), SBGA04623F1,
SAOA02667F1 74 153 2966260 SCORNOT04 530242H1 (BRAINOT03),
2113607H1 (BRAITUT03), 2125619F6 (BRSTNOT07), 2155349H1 and
2156022H1 (BRAINOT09), 2966260F6, 2966260H1, and 2966260T6
(SCORNOT04), 3270731H1 (BRAINOT20), 3272328F6 (PROSBPT06) 75 154
2993326 KIDNFET02 190217F1 (SYNORAB01), 815990R1 and 815990T1
(OVARTUT01), 2993326H1 (KIDNFET02), 3629860H1 (COLNNOT38) 76 155
3001124 TLYMNOT06 2123347T6 (BRSTNOT07), 3001124H1 (TLYMNOT06),
SBEA07088F3 77 156 3120070 LUNGTUT13 021565F1 (ADENINB01), 144798R1
(TLYMNOR01), 1216676H1 (BRSTTUT01), 2024357H1 (KERANOT02),
2616322H1 (GBLANOT01), 2742604H1 (BRSTTUTI4), 2746025H1
(LUNGTUT11), 2924884H1 (SININOT04), 3120070H1 (LUNGTUT13) 78 157
3133035 SMCCNOT01 1478001F1 and 1482667H1 (CORPNOT02), 2812193F6
and 2812193T6 (OVARNOT10), 3133035H1 and 3133035T6 (SMCCNOT01),
5025075F6 (OVARNON03) 79 158 3436879 PENCNOT05 3323031F6
(PTHYNOT03), 3436879F6 and 3436879H1 (PENCNOT05), 4247733H1
(BRABDIT01)
TABLE-US-00004 TABLE 2 Amino SEQ ID Acid Potential Phosphorylation
Potential Analytical NO: Residues Sites Glycosylation Sites
Signature Sequence Identification Methods 1 240 S233 S159 T194 T43
T77 T129 N73 N101 N167 S33-G36 Somatostatin receptor BLAST, T134
S171 L198-L219 tyrosine kinase BLOCKS, HMM 2 100 S6 S64
Meningioma-expressed BLAST, antigen 11 PRINTS, HMM 3 416 S14 S62
T109 T177 T340 S365 N144 N277 PMP-22/EMP/MP20 BLOCKS, S380 S6 T7
T205 S327 T331 family PRINTS, HMM Y56 4 224 T31 T57 S86 S173 S214 B
cell growth factor BLAST 5 247 S103 T60 S113 S235
5-hydroxytryptamine PRINTS receptor 6 72 Frizzled protein PRINTS,
HMM 7 106 S97 S9 S24 T31 Dopamine 2 receptor BLAST, PRINTS, HMM 8
239 S233 N230 PB39 protein BLAST, HMM 9 150 S53 S111 T127 CD44
antigen precursor PRINTS, HMM 10 110 S12 N92 Anion exchanger
BLOCKS, PRINTS, HMM 11 58 N5 N9 Neurofibromatosis BLAST, type 2
PRINTS, HMM 12 221 S35 S178 S60 S183 mitsugumin 23 BLAST, HMM 13
262 T33 S94 S150 T225 T245 T14 N104 C5a-anaphylatoxin PRINTS, HMM
S22 T30 T57 S137 T201 S207 receptor T230 14 90 S67 T52 Frizzled
protein PRINTS, HMM 15 208 T119 T123 T132 S56 S142 N121 Rieske
iron-sulphur BLOCKS, protein PRINTS, HMM 16 97 S61 T2 Endothelin B
receptor PRINTS, HMM 17 243 S82 T104 S168 T181 S6 S99 Thromboxane
receptor PRINTS, HMM T195 Y24 18 162 S26 N6 G protein-couple
BLOCKS, receptor PRINTS, HMM 19 470 S285 S29 T136 S145 T167 N118
N298 N466 R306-D308 Molluscan rhodopsin PRINTS, HMM T168 S199 S236
S249 T401 C-terminus S172 S209 S254 T264 S335 T385 20 144 S42 S21
T72 N30 N36 Lysosome-associated PRINTS, HMM membrane protein 21 221
S75 T82 S151-G154 Glycoprotein hormone BLAST, receptor PRINTS, HMM
22 688 T60 T186 T103 T298 S405 N198 N576 N577 S5-G8 Ring3 BLAST,
S484 S488 S492 S494 S498 N582 A80-N140 PRINTS S499 S503 S584 S601
S611 S647 T663 T109 T188 T284 T315 S324 S347 T402 T573 S643 T658
T681 Y118 23 439 T75 T257 S397 S424 S210 N227 S365-G368 Prostanoid
EP3 BLOCKS, S435 receptor PRINTS 24 192 S20 S44 N68 PMP-22/EMP/MP20
BLOCKS, family PRINTS, HMM 25 175 T171 T43 S136 T7 Progesterone
receptor PRINTS 26 91 S34 S19 S29 Similar to mouse BLAST,
dishevelled-3(Dvl-3). BLOCKS, PRINTS, HMM 27 214 T34 S83 T118 T152
S17 Somatostatin receptor BLOCKS, tyrosine kinasre PRINTS, HMM 28
250 S64 S132 T154 Sec22 homolog BLAST, HMM 29 84 T80 T3 S76 DPM2
protein BLAST, HMM 30 277 T140 S217 S19 S85 T129 Somatomedin B
domain BLOCKS, protein PRINTS, HMM 31 273 S64 S4 S114 S179 S256 S14
N187 Anion exchanger family BLOCKS, T167 T218 PRINTS, HMM 32 524
T190 S5 T131 S148 S171 S262 N152 N471 N501 1.46-1.67 G
protein-coupled BLOCKS, S275 T302 S356 S404 S473 N513 receptor
PRINTS, HMM S177 S207 T492 33 257 S48 S52 S55 T64 S82 T90 S96 N98
N187 Nucleoporin p62 BLAST T97 S123 T129 T144 S192 homolog S224
T227 S250 34 274 S16 T84 S249 S56 S113 N234 Molluscan rhodopsin
PRINTS C-terminus 35 281 S52 T150 S165 S263 T48 S116 G125-S132
ABC-2 type transport BLOCKS, T167 T226 T241 S185-G188 protein
PRINTS, HMM 36 335 S96 T113 T131 T308 T14 T146 N104 N111 E296 to
A307 pregnancy-specific Blast, BLOCKS, T292 S302 S312 T317 Y258
R127 to G129 beta 1-glycoprotein PRINTS, Motifs 4 precursor 37 280
T41 S102 T135 S148 N35 N53 N127 T56 to Y70 lysosomal membrane
Blast, BLOCKS, glycoprotein-type A PRINTS, Motifs precursor 38 210
S50 S143 S151 S63 S107 S153 Butyrophilin Blast 39 279 T90 N66 N171
Plasma membrane Blast glycoprotein CIG30 40 154 T75 S121 S48 S58
T112 Y84 G101 to G122 Pathogenesis-related Blast, BLOCKS, Y90 V115
to F130 protein PR-1 PRINTS 41 582 S160 S255 T256 S291 S292 G520 to
S527 semenogelin II Blast, Motifs S316 S351 S352 S411 S412 S471
S472 T485 S533 T559 S79 T93 S96 S151 S231 42 71 S17 T45 T50 M1 to
T50 Integral membrane BLOCKS, P5 to C29 protein PRINTS 43 102 T44
S33 T75 S6 to L24 TM4SF BLOCKS, S33 to G36 PRINTS, HMM I49 to I74
A2 to S29 44 226 S60 T3 T4 S85 T169 N46 N82 N83 I184 to R205
Cation-dependant PRINTS, HMM G128 to Q152 mannose transporter Y179
to Y201 protein 45 154 T145 T148 S33 T134 T141 M1 to A22 Frizzled
protein PRINTS, HMM S152 P56 to M78 P58 to M82 L91 to S110 L109 to
L125 46 167 S154 S3 T25 T29 T126 S140 E72 to F103 GPCR BLOCKS,
PRINTS, HMM 47 545 T257 S513 S10 T11 S47 S166 N8 N406 E376 to K410
Human secreted Blast, BLOCKS, S408 S495 protein K640 PRINTS, HMM
variant 48 570 T529 S128 S130 T184 T235 N27 N61 N75 N87 V296 to
C309 GPCR Blast, BLOCKS, T161 S293 Y199 N264 F321 to F332 PRINTS,
HMM 49 127 S24 T118 N10 to G30 Anion exchanger PRINTS, HMM 50 152
T49 S16 L78 to L99 TM4SF BLOCKS, L85 to L106 GNS1/SUR4 family HMM,
V47 to Y63 Motifs Y45 to V94 51 777 T48 S66 S162 T268 S272 T322 N64
N205 N470 T20 to D34 pecanex protein Blast, PRINTS, T355 S393 S471
S559 S574 N706 R122 to L132 Motifs S624 S660 S700 T742 S750 L598 to
L619 S11 T12 S196 S346 T400 S423 D331 to L349 T493 T579 T582 S599
S723 R565 to T582 52 108 S52 T31 T105 L76 to Y92 GNS1/SUR4 family
BLOCKS, PRINTS, PROFILESCAN 53 66 S4 S35 N2 F22 to G58 NF2 protein
Blast, BLOCKS, PRINTS, HMM 54 540 S135 S149 T527 T82 T94 T177 N50
N92 N160 N334 S115 to G118 LIV-1 protein Blast, PRINTS, S441 N395
L295 to L308 HMM, Motifs L490 to L518 55 87 T4 S13 S37 S68 S69 I46
to L82 calveolin BLOCKS, HMM 56 100 S94 I7 to N34 ammonium ion
BLOCKS, G8 to F21 transporters PRINTS, HMM K65 to N91 T78 to C97 57
58 T43 shox protein BLAST, HMM 58 61 S51 S58 S42 R2 to L23 carboxyl
ester Blast, PRINTS, lipase HMM 59 50 S9 C33 to W45 Lipoxygenase;
growth BLOCKS, C11 to L40 factor and cytokines PRINTS, receptor
family HMM, Motifs 60 310 T46 T156 S301 T81 S108 S166 A153 to S166
C4 methyl-sterol Blast, PRINTS, S305 oxidase HMM 61 160 S114 L71 to
W84 C5A-anaphylatoxin Blast, BLOCKS, Y143 to T154 receptor PRINTS,
HMM 62 35 K11 to M34 steroid hormone PRINTS receptor 63 323 T92
S105 S182 T263 S301 N90 M1-G31 Signal Peptide Signal Peptide Motifs
S271 M1-A27 Signal Peptide Containing Trans- SPScan L234-L254 TM
Protein membrane Protein HMM 64 129 T112 T117 S5 S54 M1-G27 Signal
Peptide Signal Peptide Motifs M1-G27 Signal Peptide Containing
Trans- SPScan I81-V100 TM Prot. membrane Protein HMM 65 461 T56 T41
S47 T56 T127 S146 N193 N236 Signal Peplide Motifs S147 S197 S198
T407 S8 S47 Containing Trans- T51 T284 T341 T407 membrane Protein
66 264 S243 T264 S33 T211 S260 S22 N172 N250 M1-A17 Signal Peptide
Protein Splicing Motifs S243 S260 M1-S22 Signal Peptide Protein
SPScan L173-Y195TM Prot. HMM M1-L21 TM Prot. BLOCKS L25-R30 Prot.
Splicing 67 339 T99 S119 S157 S166 S321 T54 N172 M1-G30 Signal
Peptide Signal Peptide Motifs S55 T77 S149 S211 S279 T336 M1-G26
Signal Peptide Containing Trans- SPScan Y105 L176-L194 TM. Prot.
membrane Protein HMM 68 397 S104 T148 T166 T259 S303 G202-S209
ATP/GTP Gene Regulatory Motifs S317 T127 T191 S302 binding Protein
SPScan L10-L31 Leucine zipper BLAST D106-L108 Ca binding HMM
S367-L384 Signal Peptide M1-G29 Transmembr. Prot. 69 301 T7 S52
S100 S133 S239 T155 N162 N211 V12-A32 TM. Prot. Aminoacyl tRNA
Motifs T206 V282-G300 TMr. Prot. ligase HMM L59-V64 aatRNA ligase
BLOCKS 70 217 S8 S142 T112 T197 W73-I99 TM. Prot. Cell
Proliferation Motifs Protein HMM 71 143 S81 T120 S139 S116 M1-C26
Signal Peptide Signal Peptide Motifs M1-R25 Signal Peptide
Containing Trans- SPScan M1-V22 TM Prot. membrane Protein HMM 72
186 T50 S132 T151 S116 Y43 N29 N104 M1-S25 Signal Peptide T-cell
Receptor Motifs M1-S31 Signal Peptide Interacting SPScan F9-F28 TM
Prot. Molecule HMM A27-G891 T-cell BLAST receptor interacting
molecule 73 364 S172 S213 S243 S302 N229 L234-L255 Leucine Gene
Regulatory Motifs zipper Protein SPScan M1-G28 Signal Peptide HMM
L151-L170 TM. Prot. L72-E92 TM Prot. 74 605 S46 T54 S108 S129 S195
S220 N106 N193 N395 M1-A32 Signal Peptide 2-Membrane Spanning
Motifs S231 T254 T261 S316 S440 N480 V494-I515 TM. Prot. Signal
Peptide SPScan S472 S536 S560 T124 L17-E36 TM Prot. Containing
Trans- HMM membrane Protein 75 97 T2 S87 M1-G26 Signal Peptide
2-Membrane Spanning Motifs M1-G23 Signal Peptide Signal Peptide
SPScan V35-M54 TM. Prot. Containing Trans- HMM I11-134 TM Prot.
membrane Protein 76 247 S160 T204 S165 F72-L90 Transmembr.
2-Membrane Spanning Motifs Prot. Signal Peptide HMM L45-T64
Transmembr. Containing Trans- Prot. membrane Protein 77 193 S60 S67
M1-D26 Signal Peptide Peripheral Myelin Motifs M1-A31 Signal
Peptide Protein 22 SPScan M80-M104 TM Prot. HMM R109-Y129 TM Prot.
BLOCKS S67-L108 PMP-22 Y149-Y176 PMP-22 N150-A159 Trehalase 78 128
S30 S30 S50 N71 N84 N91 N126-L128 microbodies Microbody Protein
Motifs targeting motif 79 115 S109 M1-S16 Signal Peptide G Protein
Receptor Motifs M1-T24 Signal Peptide SPScan M1-W19 TM Prot. HMM
V27-Y46 TM Prot. PRINTS V5-V15 G Prot. Receptor
TABLE-US-00005 TABLE 3 Nucleotide SEQ ID NO: Tissue Expression
(Fraction of Total) Disease Class (Fraction of Total) Vector 80
Reproductive (0.321) Cardiovascular (0.143) Cancer (0.527)
Inflammation (0.232) Fetal (0.170) pBLUESCRIPT Gastrointestinal
(0.134) 81 Cardiovascular (0.500) Gastrointestinal (0.250) Other
Cancer (0.500) Fetal (0.250) Other (0.250) pBLUESCRIPT (0.250) 82
Reproductive (0.260) Cardiovascular (0.220) Cancer (0.500)
Inflammation (0.180) Fetal (0.160) pSPORT I Gastrointestinal
(0.120) 83 Nervous (0.400) Gastrointestinal (0.300) Developmental
Cancer (0.500) Inflammation (0.300) Fetal (0.200) pINCY I (0.100)
84 Reproductive (0.266) Gastrointestinal (0.141) Cancer (0.469)
Inflammation (0.250) Fetal (0.195) pINCY I Cardiovascular (0.125)
85 Reproductive (0.750) Developmental (0.250) Cancer (0.750) Fetal
(0.250) pINCY I 86 Reproductive (0.250) Cardiovascular (0.143)
Nervous Inflammation (0.321) Trauma (0.286) Cancer (0.250) pINCY I
(0.143) 87 Reproductive (0.368) Developmental (0.158) Cancer
(0.421) Fetal (0.368) Inflammation (0.211) pINCY I Cardiovascular
(0.105) 88 Hematopoietic/Immune (0.417) Cardiovascular (0.250)
Inflammation (0.417) Cancer (0.333) Fetal (0.167) pINCY I
Reproductive (0.167) 89 Cardiovascular (0.220) Nervous (0.171)
Reproductive Cancer (0.463) Inflammation (0.195) Trauma (0.171)
pINCY I (0.122) 90 Gastrointestinal (0.200) Reproductive (0.200)
Urologic Cancer (0.500) Inflammation (0.300) Other (0.100) pINCY I
(0.200) 91 Reproductive (0.306) Cardiovascular (0.204) Nervous
Cancer (0.510) Inflammation (0.204) Fetal (0.143) pINCY I (0.122)
92 Reproductive (0.227) Hematopoietic/Immune (0.182) Cancer (0.432)
Fetal (0.273) Inflammation (0.273) pINCY I Cardiovascular (0.136)
93 Gastrointestinal (0.375) Reproductive (0.188) Cancer (0.500)
Inflammation (0.250) Trauma (0.125) pINCY I Cardiovascular (0.125)
94 Reproductive (0.333) Cardiovascular (0.214) Cancer (0.548)
Inflammation (0.167) Fetal (0.143) pINCY I Gastrointestinal (0.143)
95 Cardiovascular (0.231) Gastrointestinal (0.231) Cancer (0.500)
Inflammation (0.231) Fetal (0.154) pINCY I Reproductive (0.192) 96
Gastrointestinal (0.208) Cardiovascular (0.167) Cancer (0.542)
Inflammation (0.292) Other (0.083) pINCY I Reproductive (0.167) 97
Hematopoietic/Immune (0.341) Reproductive (0.268) Cancer (0.415)
Inflammation (0.415) Fetal (0.195) pINCY I Cardiovascular (0.122)
98 Gastrointestinal (0.346) Reproductive (0.231) Inflammation
(0.462) Cancer (0.385) Fetal (0.115) pSPORT I Hematopoietic/Immune
(0.154) 99 Gastrointestinal (0.400) Developmental (0.200) Nervous
Cancer (0.400) Fetal (0.200) Neurological (0.200) pSPORT I (0.200)
100 Reproductive (0.231) Nervous (0.168) Cardiovascular Cancer
(0.441) Inflammation (0.231) Fetal (0.133) pSPORT 1 (0.140) 101
Hematopoietic/Immune (0.225) Reproductive (0.225) Cancer (0.475)
Inflammation (0.325) Fetal (0.175) pINCY I Gastrointestinal (0.125)
102 Reproductive (0.333) Gastrointestinal (0.185) Nervous Cancer
(0.630) Fetal (0.185) Inflammation (0.111) pINCY I (0.148) 103
Gastrointestinal (0.242) Reproductive (0.182) Cancer (0.455)
Inflammation (0.364) Fetal (0.182) pINCY I Developmental (0.121)
104 Gastrointestinal (0.188) Hematopoietic/Immune (0.188)
Inflammation (0.438) Cancer (0.281) Fetal (0.250) pINCY I Urologic
(0.188) 105 Urologic (0.250) Cardiovascular (0.167)
Gastrointestinal Fetal (0.500) Cancer (0.417) Inflammation (0.333)
pINCY I (0.167) 106 Hematopoietic/Immune (0.333) Urologic (0.333)
Cancer (0.333) Fetal (0.333) Inflammation (0.333) pINCY I 107
Reproductive (0.286) Cardiovascular (0.204) Nervous Cancer (0.592)
Fetal (0.143) Inflammation (0.143) pINCY I (0.184) 108 Reproductive
(0.231) Gastrointestinal (0.215) Cancer (0.462) Inflammation
(0.292) Fetal (0.185) pINCY I Hematopoietic/Immune (0.154) 109
Reproductive (0.304) Cardiovascular (0.261) Cancer (0.609)
Inflammation (0.174) Trauma (0.087) pINCY I Gastrointestinal
(0.130) 110 Reproductive (0.256) Gastrointestinal (0.186) Cancer
(0.558) Inflammation (0.349) Trauma (0.070) pINCY I
Hematopoietic/Immune (0.186) 111 Nervous (0.200) Reproductive
(0.200) Gastrointestinal Cancer (0.550) Fetal (0.175) Inflammation
(0.150) pINCY I (0.175) 112 Developmental (0.222) Endocrine (0.222)
Cancer (0.222) Inflammation (0.222) Fetal (0.222) pINCY I
Hematopoietic/Immune (0.222) 113 Hematopoietic/Immune (0.267)
Nervous (0.200) Cancer (0.467) Trauma (0.267) Inflammation (0.200)
pINCY I Gastrointestinal (0.133) 114 Hematopoietic/Immune (0.304)
Gastrointestinal (0.130) Inflammation (0.391) Cancer (0.304) Fetal
(0.130) pINCY I Nervous (0.130) 115 Developmental (0.333)
Cardiovascular (0.167) Fetal (0.667) Inflammation (0.500)
pBLUESCRIPT Dermatologic (0.167) 116 Nervous (0.478)
Gastrointestinal (0.130) Cancer (0.565) Fetal (0.217) Inflammation
(0.217) pBLUESCRIPT Hematopoietic/Immune (0.130) 117 Reproductive
(0.222) Hematopoietic/Immune (0.200) Cancer (0.422) Inflammation
(0.311) Fetal (0.178) pINCY Nervous (0.156) 118 Reproductive
(0.256) Gastrointestinal (0.148) Nervous Cancer (0.430)
Inflammation (0.259) Fetal (0.196) pSPORT1 (0.125) 119 Reproductive
(0.190) Nervous (0.167) Developmental Cancer (0.381) Inflammation
(0.333) Fetal (0.262) pINCY (0.143) 120 Reproductive (0.800)
Urologic (0.100) Cancer (0.900) Trauma (0.100) pINCY 121
Reproductive (0.295) Nervous (0.182) Cardiovascular Cancer (0.455)
Inflammation (0.182) pBLUESCRIPT (0.159) Cell Proliferation (0.159)
122 Developmental (0.250) Musculoskeletal (0.250) Nervous Cancer
(0.500) Cell Proliferation (0.250) Inflammation pINCY (0.250)
(0.250) 123 Gastrointestinal (0.786) Developmental (0.071) Nervous
Cancer (0.500) Inflammation (0.429) pINCY (0.071) Cell
Proliferation (0.071) 124 Reproductive (0.348) Cardiovascular
(0.159) Cancer (0.493) Inflammation (0.246) pINCY
Hematopoietic/Immune (0.130) Cell Proliferation (0.145) 125 Nervous
(0.405) Reproductive (0.324) Cardiovascular Cancer (0.459)
Proliferation (0.189) Inflammation (0.108) pINCY (0.108) 126
Reproductive (0.275) Nervous (0.231) Gastrointestinal Cancer
(0.549) Inflammation (0.220) pINCY (0.154) Cell Proliferation
(0.154) 127 Reproductive (0.250) Nervous (0.150) Cardiovascular
Cancer (0.517) Cell Proliferation (0.350) Inflammation pINCY
(0.133) (0.233) 128 Nervous (0.333) Reproductive (0.333) Cancer
(0.593) Inflammation (0.259) Neurological pINCY
Hematopoietic/Immune (0.111) (0.111) 129 Hematopoietic/Immune
(0.304) Gastrointestinal (0.214) Cancer (0.446) Inflammation
(0.446) pINCY Reproductive (0.196) Cell Proliferation (0.161) 130
Nervous (0.400) Reproductive (0.300) Endocrine (0.100) Cancer
(0.300) Inflammation (0.300) pBLUESCRIPT Cell Proliferation (0.200)
131 Reproductive (0.364) Cardiovascular (0.227) Nervous Cancer
(0.545) Inflammation (0.318) pSPORT1 (0.227) Cell Proliferation
(0.091) 132 Cardiovascular (0.667) Nervous (0.333) Cell
Proliferation (1.000) Cancer (0.333) pINCY 133 Gastrointestinal
(0.750) Developmental (0.125) Cancer (0.375) Cell Proliferation
(0.292) Inflammation pINCY Reproductive (0.083) (0.250) 134
Cardiovascular (0.250) Developmental (0.250) Cancer (0.500) Cell
Proliferation (0.500) Inflammation pINCY Gastrointestinal (0.250)
(0.250) 135 Reproductive (0.250) Nervous (0.208) Endocrine (0.167)
Inflammation (0.417) Cancer (0.208) Trauma (0.167) pINCY 136
Developmental (0.500) Reproductive (0.500) Cancer (0.500) Cell
Proliferation (0.500) pINCY 137 Developmental (1.000) Cell
Proliferation (1.000) pINCY 138 Developmental (0.333) Endocrine
(0.333) Gastrointestinal Cancer (0.666) Fetal (0.333) pINCY (0.333)
139 Reproductive (0.538) Developmental (0.154) Cancer (0.462)
Inflammation (0.231) pINCY Gastrointestinal (0.154) Cell
Proliferation (0.154) 140 Gastrointestinal (0.385) Endocrine
(0.231) Reproductive Cancer (0.308) Inflammation (0.308) pINCY
(0.231) Cell Proliferation (0.077) 141 Nervous (0.500)
Cardiovascular (0.167) Gastrointestinal Cancer (0.333) Trauma
(0.333) Neurological (0.167) pINCY (0.167) 142 Reproductive (0.220)
Gastrointestinal (0.155) Nervous Cell Proliferation (0.637)
Inflammation (0.312) pBLUESCRIPT (0.152) 143 Cardiovascular (0.202)
Reproductive (0.190) Cell Proliferation (0.583) Inflammation
(0.322) pBLUESCRIPT Gastrointestinal (0.179) 144 Reproductive
(0.242) Nervous (0.158) Gastrointestinal Cell Proliferation (0.632)
Inflammation (0.379) pINCY (0.116) 145 Cardiovascular (0.238)
Reproductive (0.238) Nervous Cell Proliferation (0.619)
Inflammation (0.476) pINCY (0.143) 146 Reproductive (0.235) Nervous
(0.189) Cell Proliferation (0.625) Inflammation (0.348) pINCY
Hematopoietic/Immune (0.131) 147 Reproductive (0.191)
Hematopoietic/Immune (0.173) Cell Proliferation (0.582)
Inflammation (0.455) pINCY Nervous (0.145) 148 Reproductive (0.279)
Hematopoietic/Immune (0.140) Cell Proliferation (0.674)
Inflammation (0.232) pINCY Nervous (0.128) 149 Reproductive (0.286)
Nervous (0.214) Cardiovascular Cell Proliferation (0.834)
Inflammation (0.215) pINCY (0.095) 150 Hematopoietic/Immune (0.400)
Endocrine (0.200) Cell Proliferation (0.200) Inflammation (0.800)
pINCY Gastrointestinal (0.200) 151 Hematopoietic/Immune (0.667)
Gastrointestinal (0.167) Cell Proliferation (0.167) Inflammation
(0.667) pINCY Musculoskeletal (0.167) 152 Reproductive (0.240)
Nervous (0.173) Cell Proliferation (0.546) Inflammation (0.360)
pINCY Hematopoietic/Immune (0.133) 153 Reproductive (0.308) Nervous
(0.231) Gastrointestinal Cell Proliferation (0.885) Inflammation
(0.154) pINCY (0.115) 154 Nervous (0.455) Reproductive (0.182)
Developmental Cell Proliferation (0.682) Inflammation (0.181) pINCY
(0.136) 155 Reproductive (0.286) Urologic (0.286) Cardiovascular
Cell Proliferation (0.857) Inflammation (0.429) pINCY (0.143) 156
Reproductive (0.299) Gastrointestinal (0.216) Cell Proliferation
(0.767) Inflammation (0.246) pINCY Cardiovascular (0.120) 157
Nervous (0.222) Reproductive (0.222) Cell Proliferation (0.333)
Inflammation (0.222) pINCY 158 Reproductive (0.429) Nervous (0.357)
Cell Proliferation (0.286) Inflammation (0.357) pINCY
TABLE-US-00006 TABLE 4 Clone ID Library Library Comment Nu- cleo-
tide SEQ ID NO: 80 153831 THPIPLB02 The THPIPLB02 library was
constructed by reamplification of THPIPLB01, which was made using
RNA isolated from THP-1 cells cultured for 48 hours with 100 ng/ml
phorbol ester (PMA), followed by a 4-hour culture in media
containing 1 g/ml LPS. THP-1 (ATCC TIB 202) is a human promonocyte
line derived from the peripheral blood of a 1-year-old male with
acute monocytic leukemia (ref: Int. J. Cancer (1980) 26: 171). 81
350629 LVENNOT01 The LVENNOT01 library was constructed using RNA
isolated from the left ventricle of a 51-year-old Caucasian female,
who died from an intracranial bleed. 82 729171 LUNGNOT03 The
LUNGNOT03 library was constructed using polyA RNA isolated from
nontumorous lung tissue of a 79-year- old Caucasian male. Tissue
had been removed from the upper and lower left lobes of the lung,
superior (left paratracheal) and inferior (subclavian) mediastinal
lymph nodes, and the right paratracheal region. Pathology for the
associated tumor tissue indicated grade 4 carcinoma. Patient
history included a benign prostate neoplasm, atherosclerosis,
benign hypertension, and tobacco use. 83 1273641 TESTTUT02 The
TESTTUT02 library was constructed using polyA RNA isolated from a
testicular tumor removed from a 31-year-old Caucasian male during
unilateral orchiectomy. Pathology indicated embryonal carcinoma
forming a largely necrotic mass involving the entire testicle. Rare
foci of residual testicle showed intralobular germ cell neoplasia
and tumor was identified at the spermatic cord margin. 84 1427389
SINTBST01 The SINTBST01 library was constructed using polyA RNA
isolated from the ileum tissue of an 18-year-old Caucasian female
with irritable bowel syndrome (IBS). Pathology indicated Crohn's
disease of the ileum, involving 15 cm of the small bowel. Patient
history included osteoporosis of the vertebra and abnormal blood
chemistry. Family history included cerebrovascular disease and
atherosclerotic coronary artery disease. 85 1458357 COLNFET02 The
COLNFET02 library was constructed using RNA isolated from the colon
tissue of a Caucasian female fetus, who died at 20 weeks' gestation
from fetal demise. Serology was negative. 86 1482837 CORPNOT02 The
CORPNOT02 library was constructed using polyA RNA isolated from
diseased corpus callosum tissue removed from the brain of a
74-year-old Caucasian male, who died from Alzheimer's disease.
Serologies were negative. Pro- tein SEQ ID NO: 87 1517434 PANCTUT01
The PANCTUT01 library was constructed using polyA RNA isolated from
pancreatic tumor tissue removed from a 65-year-old Caucasian female
during radical subtotal pancreatectomy. Pathology indicated an
invasive grade 2 adenocarcinoma. Patient history included
osteoarthritis, benign hypertension, atherosclerotic coronary
artery disease, an acute myocardial infarction, benign neoplasm in
the large bowel, and a cataract disorder. Family history included
benign hypertension and atherosclerotic coronary artery disease,
Type II diabetes, impaired renal function, and stomach cancer. 88
1536052 SPLNNOT04 The SPLNNOT04 library was constructed using polyA
RNA isolated from the spleen tissue of a 2-year-old Hispanic male,
who died from cerebral anoxia. Past medical history and serologies
were negative. 89 1666118 BRSTNOT09 The BRSTNOT09 library was
constructed using polyA RNA isolated from nontumor breast tissue
removed from a 45-year-old Caucasian female during unilateral
extended simple mastectomy. Pathology for the associated tumor
tissue indicated invasive nuclear grade 2-3 adenocarcinoma in the
same breast, with 3 of 23 lymph nodes positive for metastatic
disease. There were also positive estrogen/progesterone receptors
and uninvolved tissue showing proliferative changes. Patient
history included valvuloplasty of mitral valve without replacement,
rheumatic mitral insufficiency, rheumatic heart disease, and
tobacco use. Family history included acute myocardial infarction,
atherosclerotic coronary artery disease, and Type II diabetes. 90
1675560 BLADNOT05 The BLADNOT05 library was constructed using polyA
RNA isolated from nontumorous bladder tissue removed from a
60-year-old Caucasian male during a radical cystectomy,
prostatectomy, and vasectomy. Pathology for the associated tumor
tissue indicated grade 3 transitional cell carcinoma. The patient
presented with dysuria. Family history included Type I diabetes, a
malignant neoplasm of the stomach, atherosclerotic coronary artery
disease, and an acute myocardial infarction. 91 1687323 PROSTUT10
The PROSTUT10 library was constructed using polyA RNA isolated from
prostatic tumor tissue removed from a 66-year-old Caucasian male
during radical prostatectomy and regional lymph node excision.
Pathology indicated an adenocarcinoma (Gleason grade 2 + 3).
Adenofibromatous hyperplasia was also present. The patient
presented with elevated prostate specific antigen (PSA). Family
history included prostate cancer, secondary bone cancer, and benign
hypertension. 92 1692236 PROSTUT10 The PROSTUT10 library was
constructed using polyA RNA isolated from prostatic tumor tissue
removed from a 66-year-old Caucasian male during radical
prostatectomy and regional lymph node excision. Pathology indicated
an adenocarcinoma (Gleason grade 2 + 3). Adenofibromatous
hyperplasia was also present. The patient presented with elevated
prostate specific antigen (PSA). Family history included prostate
cancer, secondary bone cancer, and benign hypertension. 93 1720847
BLADNOT06 The BLADNOT06 library was constructed using polyA RNA
isolated from the posterior wall bladder tissue removed from a
66-year-old Caucasian male during a radical prostatectomy, radical
cystectomy, and urinary diversion. Pathology for the associated
tumor tissue indicated grade 3 transitional cell carcinoma. The
patient presented with prostatic inflammatory disease. Family
history included a malignant breast neoplasm, benign hypertension,
cerebrovascular disease, atherosclerotic coronary artery disease,
and lung cancer. 94 1752821 LIVRTUT01 The LIVRTUT01 library was
constructed using polyA RNA isolated from liver tumor tissue
removed from a 51-year-old Caucasian female during a hepatic
lobectomy. Pathology indicated metastatic grade 3 adenocarcinoma
consistent with colon cancer. Patient history included
thrombophlebitis and pure hypercholesterolemia. Patient medications
included Premarin and Provera. The patient had also received 8
cycles of fluorouracil and leucovorin in the two years prior to
surgery. Family history included a malignant neoplasm of the liver.
95 1810923 PROSTUT12 The PROSTUT12 library was constructed using
polyA RNA isolated from prostate tumor tissue removed from a
65-year-old Caucasian male during a radical prostatectomy.
Pathology indicated an adenocarcinoma (Gleason grade 2 + 2).
Adenofibromatous hyperplasia was also present. The patient
presented with elevated prostate specific antigen (PSA). 96 1822315
GBLATUT01 The GBLATUT01 library was constructed using polyA RNA
isolated from gallbladder tumor tissue removed from a 78-year-old
Caucasian female during a cholecystectomy. Pathology indicated
invasive grade 3 transitional cell carcinoma. The patient was
taking Indural (propranolol hydrochloride) for hypertension. Family
history included a cholecystectomy, atherosclerosis,
hyperlipidemia, and benign hypertension. 97 1877777 LEUKNOT03 The
LEUKNOT03 library was constructed using polyA RNA isolated from
white blood cells of a 27-year-old female with blood type A+. The
donor tested negative for cytomegalovirus (CMV). 98 1879819
LEUKNOT03 The LEUKNOT03 library was constructed using polyA RNA
isolated from white blood cells of a 27-year-old female with blood
type A+. The donor tested negative for cytomegalovirus (CMV). 99
1932945 COLNNOT16 The COLNNOT16 library was constructed using polyA
RNA isolated from nontumorous sigmoid colon tissue removed from a
62-year-old Caucasian male during a sigmoidectomy and permanent
colostomy. Pathology for the associated tumor tissue indicated
invasive grade 2 adenocarcinoma. Family history included benign
hypertension, atherosclerotic coronary artery disease,
hyperlipidemia, breast cancer, and prostate cancer. 100 2061026
OVARNOT03 The OVARNOT03 library was constructed using polyA RNA
isolated from nontumorous ovarian tissue removed from a 43-year-old
Caucasian female during a bilateral salpingo-oopherectomy.
Pathology for the associated tumor tissue indicated grade 2
mucinous cystadenocarcinoma. Family history included
atherosclerotic coronary artery disease, pancreatic cancer, stress
reaction, cerebrovascular disease, breast cancer, and uterine
cancer. 101 2096687 BRAITUT02 The BRAITUT02 library was constructed
using polyA RNA isolated from brain tumor tissue removed from the
frontal lobe of a 58-year-old Caucasian male during excision of a
cerebral meningeal lesion. Pathology indicated a grade 2 metastatic
hypernephroma. Patient history included a grade 2 renal cell
carcinoma, insomnia, and chronic airway obstruction. Previous
surgeries included a nephroureterectomy. Patient medications
included Decadron (dexamethasone) and Dilantin (phenytoin). Family
history included a malignant neoplasm of the kidney. 102 2100530
BRAITUT02 The BRAITUT02 library was constructed using polyA RNA
isolated from brain tumor tissue removed from the frontal lobe of a
58-year-old Caucasian male during excision of a cerebral meningeal
lesion. Pathology indicated a grade 2 metastatic hypernephroma.
Patient history included a grade 2 renal cell carcinoma, insomnia,
and chronic airway obstruction. Previous surgeries included a
nephroureterectomy. Patient medications included Decadron
(dexamethasone) and Dilantin (phenytoin). Family history included a
malignant neoplasm of the kidney. 103 2357636 LUNGNOT20 The
LUNGNOT20 library was constructed using polyA RNA isolated from
lung tissue removed from the right upper lobe a 61-year-old
Caucasian male during a segmental lung resection. Pathology
indicated panacinal emphysema. Family history included a subdural
hemorrhage, cancer at an unidentified site, benign hypertension,
atherosclerotic coronary artery disease, pneumonia, and an
unspecified muscle disorder. 104 2365230 ADRENOT07 The ADRENOT07
library was constructed using polyA RNA isolated from adrenal
tissue removed from a 61-year-old female during a bilateral
adrenalectomy. Patient history included an unspecified disorder of
the adrenal glands, depressive disorder, benign hypertension, vocal
cord paralysis, hemiplegia, subarachnoid hemorrhage, communicating
hydrocephalus, neoplasm of uncertain behavior of pituitary gland,
hyperlipidemia, Type II diabetes, a benign neoplasm of the colon,
osteoarthritis, Meckel's diverticulum, and tobacco use. Previous
surgeries included total excision of the pituitary gland and a
unilateral thyroid lobectomy. Patient medications included Calderol
and Premarin (conjugated estrogen). Family history included
prostate cancer, benign hypertension, myocardial infarction,
atherosclerotic coronary artery disease, congestive heart failure,
hyperlipidemia, depression, anxiety disorder, colon cancer, and gas
gangrene. 105 2455121 ENDANOT01 The ENDANOT01 library was
constructed using polyA RNA isolated from aortic endothelial cell
tissue from an explanted heart removed from a male during a heart
transplant. 106 2472514 THPINOT03 The THPINOT03 library was
constructed using polyA RNA isolated from untreated THP-1 cells.
THP-1 (ATCC TIB 202) is a human promonocyte line derived from the
peripheral blood of a 1-year-old Caucasian male with acute
monocytic leukemia (ref: Int. J. Cancer (1980) 26: 171). 107
2543486 UTRSNOT11 The UTRSNOT11 library was constructed using polyA
RNA isolated from uterine myometrial tissue removed from a
43-year-old female during a vaginal hysterectomy and
salpingo-oopherectomy. The endometrium was in proliferative phase.
Family history included benign hypertension, hyperlipidemia, colon
cancer, Type II diabetes, and atherosclerotic coronary artery
disease. 108 2778171 OVARTUT03 The OVARTUT03 library was
constructed using polyA RNA isolated from ovarian tumor tissue
removed from the left ovary of a 52-year-old mixed ethnicity female
during a total abdominal hysterectomy, bilateral
salpingo-oopherectomy, peritoneal and lymphatic structure biopsy,
regional lymph node excision, and peritoneal tissue destruction.
Pathology indicated an invasive grade 3 (of 4) seroanaplastic
carcinoma. Pathology also indicated a metastatic grade 3
seroanaplastic carcinoma. Patient history included breast cancer,
chronic peptic ulcer, joint pain, and a normal delivery. Family
history included colon cancer, cerebrovascular disease, breast
cancer, Type II diabetes, esophagus cancer, and depressive
disorder. 109 2799575 PENCNOT01 The PENCNOT01 library was
constructed using polyA RNA isolated from penis corpus cavernosum
tissue removed from a 53-year-old male. Patient history included an
untreated penile carcinoma.
110 2804955 BLADTUT08 The BLADTUT08 library was constructed using
polyA RNA isolated from bladder tumor tissue removed from a
72-year-old Caucasian male during a radical cystectomy and
prostatectomy. Pathology indicated an invasive grade 3 (of 3)
transitional cell carcinoma. Family history included myocardial
infarction, cerebrovascular disease, and brain cancer. 111 2806395
BLADTUT08 The BLADTUT08 library was constructed using polyA RNA
isolated from bladder tumor tissue removed from a 72-year-old
Caucasian male during a radical cystectomy and prostatectomy.
Pathology indicated an invasive grade 3 (of 3) transitional cell
carcinoma. Family history included myocardial infarction,
cerebrovascular disease, and brain cancer. 112 2836858 TLYMNOT03
The TLYMNOT03 library was constructed using polyA RNA isolated from
nonactivated Th1 cells. These cells were differentiated from
umbilical cord CD4 T cells with IL-12 and B7-transfected COS cells.
113 2844513 DRGLNOT01 The DRGLNOT01 library was constructed using
polyA RNA isolated from dorsal root ganglion tissue removed from
the low thoracic/high lumbar region of a 32-year-old Caucasian
male, who died from acute pulmonary edema, acute bronchopneumonia,
bilateral pleural effusions, pericardial effusion, and malignant
lymphoma (natural killer cell type). Patient medications included
Difulcan (fluconazole), Deltasone (prednisone), hydrocodone,
Lortab, Alprazolam, Reazodone, Cytabom, Etoposide, Cisplatin,
Cytarabine, and dexamethasome. The patient received radiation
therapy and multiple blood transfusions. 114 3000380 TLYMNOT06 The
TLYMNOT06 library was constructed using polyA RNA isolated from
activated Th2 cells. These cells were differentiated from umbilical
cord CD4 T cells with IL-4 in the presence of anti-IL-12 antibodies
and B7-transfected COS cells, and then activated for six hours with
anti-CD3 and anti-CD28 antibodies. 115 182532 PLACNOB01 The
PLACNOB01 library was constructed using RNA isolated from placenta.
116 239589 HIPONOT01 The HIPONOT01 library was constructed using
RNA isolated from the hippocampus tissue of a 72-year-old Caucasian
female who died from an intracranial bleed. Patient history
included nose cancer, hypertension, and arthritis. 117 1671302
BMARNOT03 The BMARNOT03 library was constructed using RNA isolated
from the left tibial bone marrow tissue of a 16-year- old Caucasian
male during a partial left tibial ostectomy with free skin graft.
Patient history included an abnormality of the red blood cells.
Family history included osteoarthritis. 118 2041858 HIPONON02 This
normalized hippocampus library was constructed from 1.13M
independent clones from HIPONOT01 library. RNA was isolated from
the hippocampus tissue of a 72-year-old Caucasian female who died
from an intracranial bleed. Patient history included nose cancer,
hypertension, and arthritis. The normalization and hybridization
conditions were adapted from Soares et al. (PNAS (1994) 91: 9928).
119 2198863 SPLNFET02 The SPLNFET02 library was constructed using
RNA isolated from spleen tissue removed from a Caucasian male
fetus, who died at 23 weeks gestation. 120 3250703 SEMVNOT03 The
SEMVNOT03 library was constructed using RNA isolated from seminal
vesicle tissue removed from a 56-year- old male during a radical
prostatectomy. Pathology for the associated tumor tissue indicated
adenocarcinoma (Gleason grade 3 + 3). 121 350287 LVENNOT01 The
LVENNOT01 library was constructed using RNA isolated from the left
ventricle of a 51-year-old Caucasian female who died from
intracranial bleeding. 122 1618171 BRAITUT12 The BRAITUT12 library
was constructed using RNA isolated from brain tumor tissue removed
from the left frontal lobe of a 40-year-old Caucasian female during
excision of a cerebral meningeal lesion. Pathology indicated grade
4 gemistocytic astrocytoma. Medications included dexamethasone and
phenytoin sodium. 123 1625863 COLNPOT01 The COLNPOT01 library was
constructed using RNA isolated from colon polyp tissue removed from
a 40-year-old Caucasian female during a total colectomy. Pathology
indicated an inflammatory pseudopolyp; this tissue was associated
with a focally invasive grade 2 adenocarcinoma and multiple
tubuvillous adenomas. Patient history included a benign neoplasm of
the bowel. Medications included Zantac, betamethasone, furosamide,
and amiodarone. 124 1638353 UTRSNOT06 The UTRSNOT06 library was
constructed using RNA isolated from myometrial tissue removed from
a 50-year-old Caucasian female during a vaginal hysterectomy.
Pathology indicated residual atypical complex endometrial
hyperplasia. Pathology for the associated tissue removed during
dilation and curettage indicated fragments of atypical complex
hyperplasia and a single microscopic focus suspicious for grade I
adenocarcinoma. Patient history included benign breast neoplasm,
hypothyroid disease, polypectomy, and arthralgia. 125 1726843
PROSNOT14 The PROSNOT14 library was constructed using RNA isolated
from diseased prostate tissue removed from a 60-year-old Caucasian
male during radical prostatectomy and regional lymph node excision.
Pathology indicated adenofibromatous hyperplasia. Pathology for the
associated tumor tissue indicated an adenocarcinoma (Gleason grade
3 + 4). The patient presented with elevated prostate specific
antigen (PSA). Patient history included a kidney cyst and
hematuria. Family history included benign hypertension,
cerebrovascular disease, and arteriosclerotic coronary artery
disease. 126 1754506 LIVRTUT01 The LIVRTUT01 library was
constructed using RNA isolated from liver tumor tissue removed from
a 51-year-old Caucasian female during a hepatic lobectomy.
Pathology indicated metastatic grade 3 adenocarcinoma consistent
with colon cancer. Medications included Premarin, Provera, and
earlier, fluorouracil, and leucovorin. Family history included a
malignant neoplasm of the liver. 127 1831378 THPIAZT01 The
THPIAZT01 library was constructed using RNA isolated from THP-1
promonocyte cells treated for 3 days with 0.8 micromolar
5-aza-2'-deoxycitidine. THP-1 (ATCC TIB 202) is a human promonocyte
line derived from peripheral blood of a one-year-old Caucasian male
with acute monocytic leukemia (Int. J. Cancer (1980) 26: 171). 128
1864943 PROSNOT19 The PROSNOT19 library was constructed using RNA
isolated from diseased prostate tissue removed from a 59- year-old
Caucasian male during a radical prostatectomy with regional lymph
node excision. Pathology indicated adenofibromatous hyperplasia.
Pathology for the associated tumor tissue indicated an
adenocarcinoma (Gleason grade 3 + 3). The patient presented with
elevated prostate-specific antigen (PSA). Family history included
benign hypertension, multiple myeloma, hyperlipidemia, and
rheumatoid arthritis. 129 1911316 CONNTUT01 The CONNTUT01 library
was constructed using RNA isolated from a soft tissue tumor removed
from the clival area of the skull of a 30-year-old Caucasian
female. Pathology indicated chondroid chordoma with neoplastic
cells reactive for keratin. Medications included
medroxyprogesterone acetate. 130 1943120 HIPONOT01 The HIPONOT01
library was constructed using RNA isolated from the hippocampus
tissue of a 72-year-old Caucasian female who died from intracranial
bleeding. Patient history included nose cancer, hypertension, and
arthritis. 131 2314236 NGANNOT01 The NGANNOT01 library was
constructed using RNA isolated from tumorous neuroganglion tissue
removed from a 9-year-old Caucasian male during a soft tissue
excision of the chest wall. Pathology indicated a ganglioneuroma
forming an encapsulated lobulated mass. The tissue from the medial
aspect pleura surrounding the tumor showed fibrotic tissue with
chronic inflammation. Family history included asthma. 132 2479409
SMCANOT01 The SMCANOT01 library was constructed using RNA isolated
from an aortic smooth muscle cell line derived from the explanted
heart of a male during a heart transplant. 133 2683149 SINIUCT01
The SINIUCT01 library was constructed using RNA isolated from ileum
tissue obtained from a 42-year-old Caucasian male during a total
intra-abdominal colectomy and endoscopic jejunostomy. Previous
surgeries included polypectomy, colonoscopy, and spinal canal
exploration. Medications included Prednisone, mesalamine, and
Deltasone. Family history included cerebrovascular disease, benign
hypertension, atherosclerotic coronary artery disease, and type II
diabetes. 134 2774051 PANCNOT15 The PANCNOT15 library was
constructed using RNA isolated from diseased pancreatic tissue
removed from a 15- year-old Caucasian male during an exploratory
laparotomy with distal pancreatectomy and total splenectomy.
Pathology indicated islet cell hyperplasia. A single pancreatic
lymph node was negative. Family history included prostate cancer
and cardiovacular disease. 135 2869038 THYRNOT10 The THYRNOT10
library was constructed using RNA isolated from the diseased left
thyroid tissue removed from a 30-year-old Caucasian female during a
unilateral thyroid lobectomy and parathyroid reimplantation.
Pathology indicated lymphocytic thyroiditis. Pathology for the
associated tumor indicated grade 1 (of 4) papillary carcinoma of
the right thyroid gland, follicular variant. Multiple perithyroidal
and other lymph nodes were negative. Patient history included
hyperlipidemia and benign ovary neoplasm. Medications included
Premarian, Provera, and Anaprox. 136 2918334 THYMFET03 The
THYMFET03 library was constructed using RNA isolated from thymus
tissue removed from a Caucasian male fetus who died at premature
birth. Serology was negative. 137 2949916 KIDNFET01 The KIDNFET01
library was constructed using RNA isolated from kidney tissue
removed from a Caucasian female fetus, who died at 17 weeks
gestation from anencephalus. Serology was negative. 138 2989375
KIDNFET02 The KIDNFET02 library was constructed using RNA isolated
from kidney tissue removed from a Caucasian male fetus who was
stillborn with a hypoplastic left heart at 23 weeks gestation.
Serology was negative. 139 3316764 PROSBPT03 The PROSBPT03 library
was constructed using RNA isolated from diseased prostate tissue
removed from a 59-year- old Caucasian male during a radical
prostatectomy and regional lymph node excision. Pathology indicated
benign prostatic hyperplasia. Pathology for the associated tumor
indicated adenocarcinoma, Gleason grade 3 + 3. The patient
presented with elevated prostate specific antigen (PSA), benign
hypertension, and hyperlipidemia. Medications included Lotensin and
Pravachol. Family history included cerebrovascular disease, benign
hypertension, and prostate cancer. 140 3359559 PROSTUT16 The
PROSTUT16 library was constructed using RNA isolated from prostate
tumor tissue removed from a 55-year- old Caucasian male. Pathology
indicated adenocarcinoma, Gleason grade 5 + 4. Adenofibromatous
hyperplasia was also present. The patient presented with elevated
prostate specific antigen (PSA). Patient history included calculus
of the kidney. Family history included lung cancer and breast
cancer. 141 4289208 BRABDIR01 The BRABDIR01 library was constructed
using RNA isolated from diseased cerebellum tissue removed from the
brain of a 57-year-old Caucasian male who died from a
cerebrovascular accident. Patient history included Huntington's
disease, emphysema, and long-term tobacco use. 142 2454013
ENDANOT01 The ENDANOT01 library was constructed using RNA isolated
from aortic endothelial cell tissue from an explanted heart removed
from a male during a heart transplant. 143 2454048 ENDANOT01 The
ENDANOT01 library was constructed using RNA isolated from aortic
endothelial cell tissue from an explanted heart removed from a male
during a heart transplant. 144 2479282 SMCANOT01 The SMCANOT01
library was constructed using RNA isolated from an aortic smooth
muscle cell line derived from the explanted heart of a male during
a heart transplant. 145 2483432 SMCANOT01 The SMCANOT01 library was
constructed using RNA isolated from an aortic smooth muscle cell
line derived from the explanted heart of a male during a heart
transplant. 146 2493824 ADRETUT05 The ADRETUT05 library was
constructed using RNA isolated from adrenal tumor tissue removed
from a 52-year- old Caucasian female during a unilateral
adrenalectomy. Pathology indicated a pheochromocytoma. 147 2555823
THYMNOT03 The THYMNOT03 library was constructed using 0.5
micrograms of polyA RNA isolated from thymus tissue removed from a
21-year-old Caucasian male during a thymectomy. Pathology indicated
an unremarkable thymus and a benign parathyroid adenoma in the
right inferior parathyroid. Patient history included atopic
dermatitis, a benign neoplasm of the parathyroid, and tobacco use.
Patient medications included multivitamins. Family history included
atherosclerotic coronary artery disease and benign hypertension.
148 2598242 OVARTUT02 The OVARTUT02 library was constructed using
RNA isolated from ovarian tumor tissue removed from a 51-year- old
Caucasian female during an exploratory laparotomy, total abdominal
hysterectomy, salpingo-oophorectomy, and an incidental
appendectomy. Pathology indicated mucinous cystadenoma presenting
as a multiloculated neoplasm involving the entire left ovary. The
right ovary contained a follicular cyst and a hemorrhagic corpus
luteum. The uterus showed proliferative endometrium and a single
intramural leiomyoma. The peritoneal biopsy indicated benign
glandular inclusions consistent with endosalpingiosis. Family
history included atherosclerotic coronary artery disease, benign
hypertension, breast cancer, and uterine cancer. 149 2634120
COLNTUT15 The COLNTUT15 library was constructed using RNA isolated
from colon tumor tissue obtained from a 64-year-old Caucasian
female during a right hemicolectomy with ileostomy and bilateral
salpingo-oophorectomy (removal of the fallopian tubes and ovaries).
Pathology indicated an invasive grade 3 adenocarcinoma. Patient
history included
hypothyroidism, depression, and anemia. Family history included
colon cancer and uterine cancer. 150 2765411 BRSTNOT12 The
BRSTNOT12 library was constructed using RNA isolated from diseased
breast tissue removed from a 32-year- old Caucasian female during a
bilateral reduction mammoplasty. Pathology indicated
nonproliferative fibrocystic disease. Family history included
benign hypertension and atherosclerotic coronary artery disease.
151 2769412 COLANOT02 The COLANOT02 library was constructed using
RNA isolated from diseased ascending colon tissue removed from a
25-year-old Caucasian female during a multiple segmental resection
of the large bowel. Pathology indicated moderately to severely
active chronic ulcerative colitis, involving the entire colectomy
specimen and sparing 2 cm of the attached ileum. Grossly, the
specimen showed continuous involvement from the rectum proximally;
marked mucosal atrophy and no skip areas were identified.
Microscopically, the specimen showed dense, predominantly mucosal
inflammation and crypt abscesses. Patient history included benign
large bowel neoplasm. 152 2842779 DRGLNOT01 The DRGLNOT01 library
was constructed using RNA isolated from dorsal root ganglion tissue
removed from the low thoracic/high lumbar region of a 32-year-old
Caucasian male who died from acute pulmonary edema and
bronchopneumonia, bilateral pleural and pericardial effusions, and
malignant lymphoma (natural killer cell type). Patient history
included probable cytomegalovirus, infection, hepatic congestion
and steatosis, splenomegaly, hemorrhagic cystitis, thyroid
hemorrhage, and Bell's palsy. 153 2966260 SCORNOT04 The SCORNOT04
library was constructed using RNA isolated from cervical spinal
cord tissue removed from a 32- year-old Caucasian male who died
from acute pulmonary edema and bronchopneumonia, bilateral pleural
and pericardial effusions, and malignant lymphoma (natural killer
cell type). Patient history included probable cytomegalovirus,
infection, hepatic congestion and steatosis, splenomegaly,
hemorrhagic cystitis, thyroid hemorrhage, and Bell's palsy. 154
2993326 KIDNFET02 The KIDNFET02 library was constructed using RNA
isolated from kidney tissue removed from a Caucasian male fetus,
who was stillborn with a hypoplastic left heart and died at 23
weeks' gestation. 155 3001124 TLYMNOT06 The TLYMNOT06 library was
constructed using 0.5 micrograms of polyA RNA isolated from
activated Th2 cells. These cells were differentiated from umbilical
cord CD4 T cells with IL-4 in the presence of anti-IL-12 antibodies
and B7-transfected COS cells, and then activated for six hours with
anti-CD3 and anti-CD28 antibodies. 156 3120070 LUNGTUT13 The
LUNGTUT13 library was constructed using RNA isolated from tumorous
lung tissue removed from the right upper lobe of a 47-year-old
Caucasian male during a segmental lung resection. Pathology
indicated invasive grade 3 (of 4) adenocarcinoma. Family history
included atherosclerotic coronary artery disease, and type II
diabetes. 157 3133035 SMCCNOT01 The SMCCNOT01 library was
constructed using RNA isolated from smooth muscle cells removed
from the coronary artery of a 3-year-old Caucasian male. 158
3436879 PENCNOT05 The PENCNOT05 library was constructed using RNA
isolated from penis left corpus cavernosum tissue.
TABLE-US-00007 TABLE 5 Parameter Program Description Reference
Threshold ABI/ A program that removes vector sequences and masks
Perkin-Elmer Applied Biosystems, FACTURA ambiguous bases in nucleic
acid sequences. Foster City, CA. ABI/ A Fast Data Finder useful in
comparing and annotating Perkin-Elmer Applied Biosystems, Mismatch
PARACEL amino acid or nucleic acid sequences. Foster City, CA;
Paracel Inc., Pasadena, CA. <50% FDF ABI/Auto A program that
assembles nucleic acid sequences. Perkin-Elmer Applied Biosystems,
Assembler Foster City, CA. BLAST A Basic Local Alignment Search
Tool useful in sequence Altschul, S. F. et al. (1990) J. Mol. Biol.
ESTs: similarity search for amino acid and nucleic acid sequences.
215: 403-410; Altschul, S. F. et al. (1997) Probability BLAST
includes five functions: blastp, blastn, blastx, Nucleic Acids Res.
25: 3389-3402. value = 1.0E-8 tblastn, and tblastx. or less Full
Length sequences: Probability value = 1.0E-10 or less FASTA A
Pearson and Lipman algorithm that searches for Pearson, W. R. and
D. J. Lipman (1988) Proc. ESTs: similarity between a query sequence
and a group of Natl. Acad Sci. 85: 2444-2448; Pearson, W. R. fasta
E value = sequences of the same type. FASTA comprises as least
(1990) Methods Enzymol. 183: 63-98; and 1.06E-6 five functions:
fasta, tfasta, fastx, tfastx, and ssearch. Smith, T. F. and M. S.
Waterman (1981) Adv. Assembled Appl. Math. 2: 482-489. ESTs: fasta
Identity = 95% or greater and Match length = 200 bases or greater;
fastx E value = 1.0E-8 or less Full Length sequences: fastx score =
100 or greater BLIMPS A BLocks IMProved Searcher that matches a
sequence Henikoff, S and J. G. Henikoff, Nucl. Acid Res., Score =
1000 against those in BLOCKS and PRINTS databases to search 19:
6565-72, 1991. J. G. Henikoff and S. Henikoff or greater; for gene
families, sequence homology, and structural (1996) Methods Enzymol.
266: 88-105; Ratio of Score/ fingerprint regions. and Attwood, T.
K. et al. (1997) J. Chem. Inf. Strength = 0.75 Comput. Sci. 37:
417-424. or larger; and Probability value = 1.0E-3 or less PFAM A
Hidden Markov Models-based application useful for Krogh, A. et al.
(1994) J. Mol. Biol., 235: 1501-1531; Score = protein family
search. Sonnhammer, E. L. L. et al. (1988) 10-50 bits, Nucleic
Acids Res. 26: 320-322. depending on individual protein families
ProfileScan An algorithm that searches for structural and sequence
Gribskov, M. et al. (1988) CABIOS 4: 61-66; Score = 4.0 motifs in
protein sequences that match sequence patterns Gribskov, et al.
(1989) Methods Enzymol. or greater defined in Prosite. 183:
146-159; Bairoch, A. et al. (1997) Nucleic Acids Res. 25: 217-221.
Phred A base-calling algorithm that examines automated Ewing, B. et
al. (1998) Genome sequencer traces with high sensitivity and
probability. Res. 8: 175-185; Ewing, B. and P. Green (1998) Genome
Res. 8: 186-194. Phrap A Phils Revised Assembly Program including
SWAT and Smith, T. F. and M. S. Waterman (1981) Adv. Score = 120
CrossMatch, programs based on efficient implementation of Appl.
Math. 2: 482-489; Smith, T. F. and M. S. Waterman or greater; the
Smith-Waterman algorithm, useful in searching (1981) J. Mol. Biol.
147: 195-197; Match length = sequence homology and assembling DNA
sequences. and Green, P., University of Washington. 56 or greater
Seattle, WA. Consed A graphical tool for viewing and editing Phrap
assemblies Gordon, D. et al. (1998) Genome Res. 8: 195-202. SPScan
A weight matrix analysis program that scans protein Nielson, H. et
al. (1997) Protein Engineering Score = 5 sequences for the presence
of secretory signal peptides. 10: 1-6; Claverie, J. M. and S. Audic
(1997) or greater CABIOS 12: 431-439. Motifs A program that
searches amino acid sequences for patterns Bairoch et al. supra;
Wisconsin that matched those defined in Prosite. Package Program
Manual, version 9, page M51-59, Genetics Computer Group, Madison,
WI.
Sequence CWU 1
1
1581240PRTHomo sapiensmisc_featureIncyte ID No 153831 1Met Gly Asn
Cys Gln Ala Gly His Asn Leu His Leu Cys Leu Ala1 5 10 15His His Pro
Pro Leu Val Cys Ala Thr Leu Ile Leu Leu Leu Leu 20 25 30Gly Leu Ser
Gly Leu Gly Leu Gly Ser Phe Leu Leu Thr His Arg 35 40 45Thr Gly Leu
Arg Ser Pro Asp Ile Pro Gln Asp Trp Val Ser Phe 50 55 60Leu Arg Ser
Phe Gly Gln Leu Thr Leu Cys Pro Arg Asn Gly Thr 65 70 75Val Thr Gly
Lys Trp Arg Gly Ser His Val Val Gly Leu Leu Thr 80 85 90Thr Leu Asn
Phe Gly Asp Gly Pro Asp Arg Asn Lys Thr Arg Thr 95 100 105Phe Gln
Ala Thr Val Leu Gly Ser Gln Met Gly Leu Lys Gly Ser 110 115 120Ser
Ala Gly Gln Leu Val Leu Ile Thr Ala Arg Val Thr Thr Glu 125 130
135Arg Thr Ala Gly Thr Cys Leu Tyr Phe Ser Ala Val Pro Gly Ile 140
145 150Leu Pro Ser Ser Gln Pro Pro Ile Ser Cys Ser Glu Glu Gly Ala
155 160 165Gly Asn Ala Thr Leu Ser Pro Arg Met Gly Glu Glu Cys Val
Ser 170 175 180Val Trp Ser His Glu Gly Leu Val Leu Thr Lys Leu Leu
Thr Ser 185 190 195Glu Glu Leu Ala Leu Cys Gly Ser Arg Leu Leu Val
Leu Gly Ser 200 205 210Phe Leu Leu Leu Phe Cys Gly Leu Leu Cys Cys
Val Thr Ala Met 215 220 225Cys Phe His Pro Arg Arg Glu Ser His Trp
Ser Arg Thr Arg Leu 230 235 2402100PRTHomo
sapiensmisc_featureIncyte ID No 350629 2Met Glu Gly Leu Arg Ser Ser
Val Glu Leu Asp Pro Glu Leu Thr1 5 10 15Pro Gly Lys Leu Asp Glu Glu
Met Val Gly Leu Pro Pro His Asp 20 25 30Ala Ser Pro Gln Val Thr Phe
His Ser Leu Asp Gly Lys Thr Val 35 40 45Val Cys Pro His Phe Met Gly
Leu Leu Leu Gly Leu Leu Leu Leu 50 55 60Leu Thr Leu Ser Val Arg Asn
Gln Leu Cys Val Arg Gly Glu Arg 65 70 75Gln Leu Ala Glu Thr Leu His
Ser Gln Val Lys Glu Lys Ser Gln 80 85 90Leu Ile Gly Lys Lys Thr Asp
Cys Arg Asp 95 1003416PRTHomo sapiensmisc_featureIncyte ID No
729171 3Met Ser Gly His Arg Ser Thr Arg Lys Arg Cys Gly Asp Ser
His1 5 10 15Pro Glu Ser Pro Val Gly Phe Gly His Met Ser Thr Thr Gly
Cys 20 25 30Val Leu Asn Lys Leu Phe Gln Leu Pro Thr Pro Pro Leu Ser
Arg 35 40 45His Gln Leu Lys Arg Leu Glu Glu His Arg Tyr Gln Ser Ala
Gly 50 55 60Arg Ser Leu Leu Glu Pro Leu Val Gln Gly Tyr Trp Glu Trp
Leu 65 70 75Val Arg Arg Val Pro Ser Trp Ile Ala Pro Asn Leu Ile Thr
Ile 80 85 90Ile Gly Leu Ser Ile Asn Ile Cys Thr Thr Ile Leu Leu Val
Phe 95 100 105Tyr Cys Pro Thr Ala Thr Glu Gln Ala Pro Leu Trp Ala
Tyr Ile 110 115 120Ala Cys Ala Cys Gly Leu Phe Ile Tyr Gln Ser Leu
Asp Ala Ile 125 130 135Gly Gly Lys Gln Ala Arg Arg Thr Asn Ser Ser
Ser Pro Leu Gly 140 145 150Glu Leu Phe Asp His Gly Cys Asp Ser Leu
Ser Thr Val Phe Val 155 160 165Val Leu Gly Thr Cys Ile Ala Val Gln
Leu Gly Thr Asn Pro Asp 170 175 180Trp Met Phe Phe Cys Cys Phe Ala
Gly Thr Phe Met Phe Tyr Cys 185 190 195Ala His Trp Gln Thr Tyr Val
Ser Gly Thr Leu Arg Phe Gly Ile 200 205 210Ile Asp Val Thr Glu Val
Gln Ile Phe Ile Ile Ile Met His Leu 215 220 225Leu Ala Val Met Gly
Gly Pro Pro Phe Trp Gln Ser Met Ile Pro 230 235 240Val Leu Asn Ile
Gln Met Lys Ile Phe Pro Ala Leu Cys Thr Val 245 250 255Ala Gly Thr
Ile Phe Pro Val Thr Asn Tyr Phe Arg Val Ile Phe 260 265 270Thr Gly
Gly Val Gly Lys Asn Gly Ser Thr Ile Ala Gly Thr Ser 275 280 285Val
Leu Ser Pro Phe Leu His Ile Gly Ser Val Ile Thr Leu Ala 290 295
300Ala Met Ile Tyr Lys Lys Ser Ala Val Gln Leu Phe Glu Lys His 305
310 315Pro Cys Leu Tyr Ile Leu Thr Phe Gly Phe Val Ser Ala Lys Ile
320 325 330Thr Asn Lys Leu Val Val Ala His Met Thr Lys Ser Glu Met
His 335 340 345Leu His Asp Thr Ala Phe Ile Gly Pro Ala Leu Leu Phe
Leu Asp 350 355 360Gln Tyr Phe Asn Ser Phe Ile Asp Glu Tyr Ile Val
Leu Trp Ile 365 370 375Ala Leu Val Phe Ser Phe Phe Asp Leu Ile Arg
Tyr Cys Val Ser 380 385 390Val Cys Asn Gln Ile Ala Ser His Leu His
Ile His Val Phe Arg 395 400 405Ile Lys Val Ser Thr Ala His Ser Asn
His His 410 4154224PRTHomo sapiensmisc_featureIncyte ID No 1273641
4Met Thr Ile Thr Ser Phe Tyr Ala Val Cys Phe Tyr Leu Leu Met1 5 10
15Leu Val Met Val Glu Gly Phe Gly Gly Lys Glu Ala Val Leu Arg 20 25
30Thr Leu Arg Asp Thr Pro Met Met Val His Thr Gly Pro Cys Cys 35 40
45Cys Cys Cys Pro Cys Cys Gln Arg Leu Leu Leu Thr Arg Lys Lys 50 55
60Leu Gln Leu Leu Met Leu Gly Pro Phe Gln Tyr Ala Phe Leu Lys 65 70
75Ile Thr Leu Thr Trp Trp Ala Leu Phe Ser Ser Pro Thr Glu Ser 80 85
90Tyr Asp Pro Ala Asp Ile Ser Glu Gly Ser Thr Ala Leu Trp Ile 95
100 105Asn Thr Phe Leu Gly Val Ser Thr Leu Leu Ala Leu Trp Thr Leu
110 115 120Gly Ile Ile Ser Arg Gln Ala Arg Leu His Leu Gly Glu Gln
Asn 125 130 135Met Gly Ala Lys Phe Ala Leu Phe Gln Val Leu Leu Ile
Leu Thr 140 145 150Ala Leu Gln Pro Ser Ile Phe Ser Val Leu Ala Asn
Gly Gly Gln 155 160 165Ile Ala Cys Ser Pro Pro Tyr Ser Ser Lys Thr
Arg Ser Gln Val 170 175 180Met Asn Cys His Leu Leu Ile Leu Glu Thr
Phe Leu Met Thr Val 185 190 195Leu Thr Arg Met Tyr Tyr Arg Arg Lys
Asp His Lys Val Gly Tyr 200 205 210Glu Thr Phe Ser Ser Pro Asp Leu
Asp Leu Asn Leu Lys Ala 215 2205247PRTHomo
sapiensmisc_featureIncyte ID No 1427389 5Met Gly Ala Ala Val Phe
Phe Gly Cys Thr Phe Val Ala Phe Gly1 5 10 15Pro Ala Phe Ala Leu Phe
Leu Ile Thr Val Ala Gly Asp Pro Leu 20 25 30Arg Val Ile Ile Leu Val
Ala Gly Ala Phe Phe Trp Leu Val Ser 35 40 45Leu Leu Leu Ala Ser Val
Val Trp Phe Ile Leu Val His Val Thr 50 55 60Asp Arg Ser Asp Ala Arg
Leu Gln Tyr Gly Leu Leu Ile Phe Gly 65 70 75Ala Ala Val Ser Val Leu
Leu Gln Glu Val Phe Arg Phe Ala Tyr 80 85 90Tyr Lys Leu Leu Lys Lys
Ala Asp Glu Gly Leu Ala Ser Leu Ser 95 100 105Glu Asp Gly Arg Ser
Pro Ile Ser Ile Arg Gln Met Ala Tyr Val 110 115 120Ser Gly Leu Ser
Phe Gly Ile Ile Ser Gly Val Phe Ser Val Ile 125 130 135Asn Ile Leu
Ala Asp Ala Leu Gly Pro Gly Val Val Gly Ile His 140 145 150Gly Asp
Ser Pro Tyr Tyr Phe Leu Thr Ser Ala Phe Leu Thr Ala 155 160 165Ala
Ile Ile Leu Leu His Thr Phe Trp Gly Val Val Phe Phe Asp 170 175
180Ala Cys Glu Arg Arg Arg Tyr Trp Ala Leu Gly Leu Val Val Gly 185
190 195Ser His Leu Leu Thr Ser Gly Leu Thr Phe Leu Asn Pro Trp Tyr
200 205 210Glu Ala Ser Leu Leu Pro Ile Tyr Ala Val Thr Val Ser Met
Gly 215 220 225Leu Trp Ala Phe Ile Thr Ala Gly Gly Ser Leu Arg Ser
Ile Gln 230 235 240Arg Ser Leu Leu Cys Lys Asp 245672PRTHomo
sapiensmisc_featureIncyte ID No 1458357 6Met Tyr Trp Leu His Gln
Asp Met Phe Trp Leu Leu Val Leu Ile1 5 10 15Leu Ile Cys Leu Val Thr
His Leu Ile Thr Arg Glu Thr Ile Tyr 20 25 30Val Lys Ser Leu Phe Tyr
Phe Lys Ile Leu Phe Val Tyr Leu Glu 35 40 45Ser Lys Pro Ala His Cys
Asn Leu Cys Leu Tyr Ala Lys Glu Leu 50 55 60Asp Phe Phe Val Phe Val
Leu Phe Phe Lys Leu Leu 65 707106PRTHomo sapiensmisc_featureIncyte
ID No 1482837 7Met His Tyr Gly Phe Leu Leu Trp Ser Gly Lys Lys Arg
Gly Leu1 5 10 15Ala Gly Pro Gln Gly Ile Cys Lys Ser Gln Lys Thr Val
Phe Leu 20 25 30Thr Ala Arg Cys His Ser Thr Leu Val Gly Lys Glu Glu
Lys Lys 35 40 45Ile Lys Leu Phe His Arg Thr Ser Trp Pro Pro His Ser
His Ala 50 55 60Leu Pro Thr Gln Pro Gly Pro Leu Pro Ala Pro Phe Ile
Lys Ala 65 70 75Glu Arg Val Glu Leu Ile Phe Thr Asn Cys Asn Ile Phe
Val Val 80 85 90Ser Val Ser Ser Phe Val Ser Ser Ala Glu Pro Cys Pro
Phe Leu 95 100 105Leu8239PRTHomo sapiensmisc_featureIncyte ID No
1517434 8Met Cys Val Thr Gln Leu Arg Leu Ile Phe Tyr Met Gly Ala
Met1 5 10 15Asn Asn Ile Leu Lys Phe Leu Val Ser Gly Asp Gln Lys Thr
Val 20 25 30Gly Leu Tyr Thr Ser Ile Phe Gly Val Leu Gln Leu Leu Cys
Leu 35 40 45Leu Thr Ala Pro Val Ile Gly Tyr Ile Met Asp Trp Arg Leu
Lys 50 55 60Glu Cys Glu Asp Ala Ser Glu Glu Pro Glu Glu Lys Asp Ala
Asn 65 70 75Gln Gly Glu Lys Lys Lys Lys Lys Arg Asp Arg Gln Ile Gln
Lys 80 85 90Ile Thr Asn Ala Met Arg Ala Phe Ala Phe Thr Asn Leu Leu
Leu 95 100 105Val Gly Phe Gly Val Thr Cys Leu Ile Pro Asn Leu Pro
Leu Gln 110 115 120Ile Leu Ser Phe Ile Leu His Thr Ile Val Arg Gly
Phe Ile His 125 130 135Ser Ala Val Gly Gly Leu Tyr Ala Ala Val Tyr
Pro Ser Thr Gln 140 145 150Phe Gly Ser Leu Thr Gly Leu Gln Ser Leu
Ile Ser Ala Leu Phe 155 160 165Ala Leu Leu Gln Gln Pro Leu Phe Leu
Ala Met Met Gly Pro Leu 170 175 180Gln Gly Asp Pro Leu Trp Val Asn
Val Gly Leu Leu Leu Leu Ser 185 190 195Leu Leu Gly Phe Cys Leu Pro
Leu Tyr Leu Ile Cys Tyr Arg Arg 200 205 210Gln Leu Glu Arg Gln Leu
Gln Gln Arg Gln Glu Asp Asp Lys Leu 215 220 225Phe Leu Lys Ile Asn
Gly Ser Ser Asn Gln Glu Ala Phe Val 230 2359150PRTHomo
sapiensmisc_featureIncyte ID No 1536052 9Met Trp Leu Pro Trp Ala
Leu Leu Leu Leu Trp Val Pro Ala Ser1 5 10 15Thr Ser Met Thr Pro Ala
Ser Ile Thr Ala Ala Lys Thr Ser Thr 20 25 30Ile Thr Thr Ala Phe Pro
Pro Val Ser Ser Thr Thr Leu Phe Ala 35 40 45Val Gly Ala Thr His Ser
Ala Ser Ile Gln Glu Glu Thr Glu Glu 50 55 60Val Val Asn Ser Gln Leu
Pro Leu Leu Leu Ser Leu Leu Ala Leu 65 70 75Leu Leu Leu Leu Leu Val
Gly Ala Ser Leu Leu Ala Trp Arg Met 80 85 90Phe Gln Lys Trp Ile Lys
Ala Gly Asp His Ser Glu Leu Ser Gln 95 100 105Asn Pro Lys Gln Ala
Ser Pro Arg Glu Glu Leu His Tyr Ala Ser 110 115 120Val Val Phe Asp
Ser Asn Thr Asn Arg Ile Ala Ala Gln Arg Pro 125 130 135Arg Glu Glu
Glu Pro Asp Ser Asp Tyr Ser Val Ile Arg Lys Thr 140 145
15010110PRTHomo sapiensmisc_featureIncyte ID No 1666118 10Met Pro
Ala Cys Ile Leu Glu Asp Val Glu Ile Ser Phe Arg Gln1 5 10 15Lys Trp
Ser Ile Asn Ser Asp Thr Leu Leu Gly Cys Leu Thr Leu 20 25 30Phe Ile
Ser Ala Phe Phe Ala Ser Glu Thr Trp Gln Lys Leu Val 35 40 45Ser Gln
Ser Thr Ala Phe Leu Thr Met Cys Gly Val Thr Tyr Ala 50 55 60Trp Tyr
Met Pro Leu Leu Leu Leu Lys Phe Tyr Ser Leu Leu Leu 65 70 75Ala Gln
Val Leu Leu Asn Pro Phe Leu Met Cys Thr Gly Trp Arg 80 85 90Lys Asn
Tyr Ser Gln His Phe Glu Arg Lys Val Phe Arg Asn Asn 95 100 105Ile
Asn Trp His Tyr 1101158PRTHomo sapiensmisc_featureIncyte ID No
1675560 11Met Leu Val Thr Asn Ile Thr Val Asn Arg Ser Leu Leu His
Ala1 5 10 15Lys Asp Gln Cys Asp Leu Trp Met Glu Met Ile Val Met Lys
Phe 20 25 30Leu Phe His Gly Ala Val Phe Leu Phe Ile Ser Leu Gly Ser
Arg 35 40 45Phe Ser Glu Ala Val Arg Cys Cys Cys Cys Gly Phe Leu 50
5512221PRTHomo sapiensmisc_featureIncyte ID No 1687323 12Met Ala
Ala Ser Ser Ile Ser Ser Pro Trp Gly Lys His Val Phe1 5 10 15Lys Ala
Ile Leu Met Val Leu Val Ala Leu Ile Leu Leu His Ser 20 25 30Ala Leu
Ala Gln Ser Arg Arg Asp Phe Ala Pro Pro Gly Gln Gln 35 40 45Lys Arg
Glu Ala Pro Val Asp Val Leu Thr Gln Ile Gly Arg Ser 50 55 60Val Arg
Gly Thr Leu Asp Ala Trp Ile Gly Pro Glu Thr Met His 65 70 75Leu Val
Ser Glu Ser Ser Ser Gln Val Leu Trp Ala Ile Ser Ser 80 85 90Ala Ile
Ser Val Ala Phe Phe Ala Leu Ser Gly Ile Ala Ala Gln 95 100 105Leu
Leu Asn Ala Leu Gly Leu Ala Gly Asp Tyr Leu Ala Gln Gly 110 115
120Leu Lys Leu Ser Pro Gly Gln Val Gln Thr Phe Leu Leu Trp Gly 125
130 135Ala Gly Ala Leu Val Val Tyr Trp Leu Leu Ser Leu Leu Leu Gly
140 145 150Leu Val Leu Ala Leu Leu Gly Arg Ile Leu Trp Gly Leu Lys
Leu 155 160 165Val Ile Phe Leu Ala Gly Phe Val Ala Leu Met Arg Ser
Val Pro 170 175 180Asp Pro Ser Thr Arg Ala Leu Leu Leu Leu Ala Leu
Leu Ile Leu 185 190 195Tyr Ala Leu Leu Ser Arg Leu Thr Gly Ser Arg
Ala Ser Gly Ala 200 205 210Gln Leu Glu Ala Lys Val Arg Gly Leu Glu
Arg 215 22013262PRTHomo sapiensmisc_featureIncyte ID No 1692236
13Met Ala Leu Gly Leu Lys Cys Phe Arg Met Val His Pro Thr Phe1 5 10
15Arg Asn Tyr Leu Ala Ala Ser Ile Arg Pro Val Ser Glu Val Thr 20 25
30Leu Lys Thr Val His Glu Arg Gln His Gly His Arg Gln Tyr Met 35 40
45Ala Tyr Ser Ala Val Pro Val Arg His Phe Ala Thr Lys Lys Ala 50 55
60Lys Ala Lys Gly Lys Gly Gln Ser Gln Thr Arg Val
Asn Ile Asn 65 70 75Ala Ala Leu Val Glu Asp Ile Ile Asn Leu Glu Glu
Val Asn Glu 80 85 90Glu Met Lys Ser Val Ile Glu Ala Leu Lys Asp Asn
Phe Asn Leu 95 100 105Thr Leu Asn Ile Arg Ala Ser Pro Gly Ser Leu
Asp Lys Ile Ala 110 115 120Val Val Thr Ala Asp Gly Lys Leu Ala Leu
Asn Gln Ile Ser Gln 125 130 135Ile Ser Met Lys Ser Pro Gln Leu Ile
Leu Val Asn Met Ala Ser 140 145 150Phe Pro Glu Cys Thr Ala Ala Ala
Ile Lys Ala Ile Arg Glu Ser 155 160 165Gly Met Asn Leu Asn Pro Glu
Val Glu Gly Thr Leu Ile Arg Val 170 175 180Pro Ile Pro Gln Val Thr
Arg Glu His Arg Glu Met Leu Val Lys 185 190 195Leu Ala Lys Gln Asn
Thr Asn Lys Ala Lys Asp Ser Leu Arg Lys 200 205 210Val Arg Thr Asn
Ser Met Asn Lys Leu Lys Lys Ser Lys Asp Thr 215 220 225Val Ser Glu
Asp Thr Ile Arg Leu Ile Glu Lys Gln Ile Ser Gln 230 235 240Met Ala
Asp Asp Thr Val Ala Glu Leu Asp Arg His Leu Ala Val 245 250 255Lys
Thr Lys Glu Leu Leu Gly 2601490PRTHomo sapiensmisc_featureIncyte ID
No 1720847 14Met Glu Ala Ala Met Glu Trp Glu Gly Gly Ala Ile Arg
His Pro1 5 10 15Ser Thr Glu Leu Gly Ile Met Gly Ser Trp Phe Tyr Leu
Phe Leu 20 25 30Ala Pro Leu Phe Lys Gly Leu Ala Gly Ser Leu Pro Phe
Gly Cys 35 40 45Leu Ser Leu Leu Gln Pro Thr Glu Lys Thr Ala Leu Gln
Arg Trp 50 55 60Arg Val Phe Met Lys His Ser Cys Gln Glu Pro Arg His
Arg Ala 65 70 75Gly Gly Leu Glu Lys Gly Gly His Thr Gly Gly Gly Arg
Ser Trp 80 85 9015208PRTHomo sapiensmisc_featureIncyte ID No
1752821 15Met Ala Ser Ser Leu Leu Ala Gly Glu Arg Leu Val Arg Ala
Leu1 5 10 15Gly Pro Gly Gly Glu Leu Glu Pro Glu Arg Leu Pro Arg Lys
Leu 20 25 30Arg Ala Glu Leu Glu Ala Ala Leu Gly Lys Lys His Lys Gly
Gly 35 40 45Asp Ser Ser Ser Gly Pro Gln Arg Leu Val Ser Phe Arg Leu
Ile 50 55 60Arg Asp Leu His Gln His Leu Arg Glu Arg Asp Ser Lys Leu
Tyr 65 70 75Leu His Glu Leu Leu Glu Gly Ser Glu Ile Tyr Leu Pro Glu
Val 80 85 90Val Lys Pro Pro Arg Asn Pro Glu Leu Val Ala Arg Leu Glu
Lys 95 100 105Ile Lys Ile Gln Leu Ala Asn Glu Glu Tyr Lys Arg Ile
Thr Arg 110 115 120Asn Val Thr Cys Gln Asp Thr Arg His Gly Gly Thr
Leu Ser Asp 125 130 135Leu Gly Lys Gln Val Arg Ser Leu Lys Ala Leu
Val Ile Thr Ile 140 145 150Phe Asn Phe Ile Val Thr Val Val Ala Ala
Phe Val Cys Thr Tyr 155 160 165Leu Gly Ser Gln Tyr Ile Phe Thr Glu
Met Ala Ser Arg Val Leu 170 175 180Ala Ala Leu Ile Val Ala Ser Val
Val Gly Leu Ala Glu Leu Tyr 185 190 195Val Met Val Arg Ala Met Glu
Gly Glu Leu Gly Glu Leu 200 2051697PRTHomo
sapiensmisc_featureIncyte ID No 1810923 16Met Thr Lys Lys Lys Arg
Glu Asn Leu Gly Val Ala Leu Glu Ile1 5 10 15Asp Gly Leu Glu Glu Lys
Leu Ser Gln Cys Arg Arg Asp Leu Glu 20 25 30Ala Val Asn Ser Arg Leu
His Ser Arg Glu Leu Ser Pro Glu Ala 35 40 45Arg Arg Ser Leu Glu Lys
Glu Lys Asn Ser Leu Met Asn Lys Ala 50 55 60Ser Asn Tyr Glu Lys Glu
Leu Lys Phe Leu Arg Gln Glu Asn Arg 65 70 75Lys Asn Met Leu Leu Ser
Val Ala Ile Phe Ile Leu Leu Thr Leu 80 85 90Val Tyr Ala Tyr Trp Thr
Met 9517243PRTHomo sapiensmisc_featureIncyte ID No 1822315 17Met
Phe Phe Leu Ser Ser Ser Lys Leu Thr Lys Trp Lys Gly Glu1 5 10 15Val
Lys Lys Arg Leu Asp Ser Glu Tyr Lys Glu Gly Gly Gln Arg 20 25 30Asn
Trp Val Gln Val Phe Cys Asn Gly Ala Val Pro Thr Glu Leu 35 40 45Ala
Leu Leu Tyr Met Ile Glu Asn Gly Pro Gly Glu Ile Pro Val 50 55 60Asp
Phe Ser Lys Gln Tyr Ser Ala Ser Trp Met Cys Leu Ser Leu 65 70 75Leu
Ala Ala Leu Ala Cys Ser Ala Gly Asp Thr Trp Ala Ser Glu 80 85 90Val
Gly Pro Val Leu Ser Lys Ser Ser Pro Arg Leu Ile Thr Thr 95 100
105Trp Glu Lys Val Pro Val Gly Thr Asn Gly Gly Val Thr Val Val 110
115 120Gly Leu Val Ser Ser Leu Leu Gly Gly Thr Phe Val Gly Ile Ala
125 130 135Tyr Phe Leu Thr Gln Leu Ile Phe Val Asn Asp Leu Asp Ile
Ser 140 145 150Ala Pro Gln Trp Pro Ile Ile Ala Phe Gly Gly Leu Ala
Gly Leu 155 160 165Leu Gly Ser Ile Val Asp Ser Tyr Leu Gly Ala Thr
Met Gln Tyr 170 175 180Thr Gly Leu Asp Glu Ser Thr Gly Met Val Val
Asn Ser Pro Thr 185 190 195Asn Lys Ala Arg His Ile Ala Gly Lys Pro
Ile Leu Asp Asn Asn 200 205 210Ala Trp Ile Cys Phe Leu Leu Phe Leu
Leu Pro Ser Cys Ser Gln 215 220 225Leu Leu Leu Gly Val Phe Gly Pro
Gly Gly Glu Leu Tyr Phe Ile 230 235 240Ser Thr Gly18162PRTHomo
sapiensmisc_featureIncyte ID No 1877777 18Met Leu Gln Thr Ser Asn
Tyr Ser Leu Val Leu Ser Leu Gln Phe1 5 10 15Leu Leu Leu Ser Tyr Asp
Leu Phe Val Asn Ser Phe Ser Glu Leu 20 25 30Leu Gln Lys Thr Pro Val
Ile Gln Leu Val Leu Phe Ile Ile Gln 35 40 45Asp Ile Ala Val Leu Phe
Asn Ile Ile Ile Ile Phe Leu Met Phe 50 55 60Phe Asn Thr Phe Val Phe
Gln Ala Gly Leu Val Asn Leu Leu Phe 65 70 75His Lys Phe Lys Gly Thr
Ile Ile Leu Thr Ala Val Tyr Phe Ala 80 85 90Leu Ser Ile Ser Leu His
Val Trp Val Met Asn Leu Arg Trp Lys 95 100 105Asn Ser Asn Ser Phe
Ile Trp Thr Asp Gly Leu Gln Met Leu Phe 110 115 120Val Phe Gln Arg
Leu Ala Ala Val Leu Tyr Cys Tyr Phe Tyr Lys 125 130 135Arg Thr Ala
Val Arg Leu Gly Asp Pro His Phe Tyr Gln Asp Ser 140 145 150Leu Trp
Leu Arg Lys Glu Phe Met Gln Val Arg Arg 155 16019470PRTHomo
sapiensmisc_featureIncyte ID No 1879819 19Met Leu Ser Pro Ser Pro
Gly Lys Gly Pro Pro Pro Ala Val Ala1 5 10 15Pro Arg Pro Lys Ala Pro
Leu Gln Leu Gly Pro Ser Ser Ser Ile 20 25 30Lys Glu Lys Gln Gly Pro
Leu Leu Asp Leu Phe Gly Gln Lys Leu 35 40 45Pro Ile Ala His Thr Pro
Pro Pro Pro Pro Ala Pro Pro Leu Pro 50 55 60Leu Pro Glu Asp Pro Gly
Thr Leu Ser Ala Glu Arg Arg Cys Leu 65 70 75Thr Gln Pro Val Glu Asp
Gln Gly Val Ser Thr Gln Leu Leu Ala 80 85 90Pro Ser Gly Ser Val Cys
Phe Ser Tyr Thr Gly Thr Pro Trp Lys 95 100 105Leu Phe Leu Arg Lys
Glu Val Phe Tyr Pro Arg Glu Asn Phe Ser 110 115 120His Pro Tyr Tyr
Leu Arg Leu Leu Cys Glu Gln Ile Leu Arg Asp 125 130 135Thr Phe Ser
Glu Ser Cys Ile Arg Ile Ser Gln Asn Glu Arg Arg 140 145 150Lys Met
Lys Asp Leu Leu Gly Gly Leu Glu Val Asp Leu Asp Ser 155 160 165Leu
Thr Thr Thr Glu Asp Ser Val Lys Lys Arg Ile Val Val Ala 170 175
180Ala Arg Asp Asn Trp Ala Asn Tyr Phe Ser Arg Phe Phe Pro Val 185
190 195Ser Gly Glu Ser Gly Ser Asp Val Gln Leu Leu Ala Val Ser His
200 205 210Arg Gly Leu Arg Leu Leu Lys Val Thr Gln Gly Pro Gly Leu
Arg 215 220 225Pro Asp Gln Leu Lys Ile Leu Cys Ser Tyr Ser Phe Ala
Glu Val 230 235 240Leu Gly Val Glu Cys Arg Gly Gly Ser Thr Leu Glu
Leu Ser Leu 245 250 255Lys Ser Glu Gln Leu Val Leu His Thr Ala Arg
Ala Arg Ala Ile 260 265 270Glu Ala Leu Val Glu Leu Phe Leu Asn Glu
Leu Lys Lys Asp Ser 275 280 285Gly Tyr Val Ile Ala Leu Arg Ser Tyr
Ile Thr Asp Asn Cys Ser 290 295 300Leu Leu Ser Phe His Arg Gly Asp
Leu Ile Lys Leu Leu Pro Val 305 310 315Cys His Pro Gly Ala Arg Leu
Ala Val Trp Leu Cys Arg Gly Pro 320 325 330Phe Arg Thr Leu Ser Cys
Arg His Ser Ala Ala Gly Cys Arg Ser 335 340 345Arg Leu Phe Leu Leu
Gln Gly Ala Glu Glu Trp Leu Ala Gln Gly 350 355 360Ser Ala Val Gln
Arg Gly Thr Arg Ala Gly Ser Val Gly Gln Gly 365 370 375Leu Arg Gly
Glu Glu Asp Gly Arg Gly Thr Ser Arg Gly Lys Ala 380 385 390Cys Leu
Arg Leu Arg Lys Glu Arg Gly Leu Thr Thr Pro Glu Ala 395 400 405Ala
Met Arg Trp Asp His Pro Ala Val Arg Leu Leu Trp Leu Pro 410 415
420Leu Cys Pro Leu Leu Met Ala Arg Leu Val Ser Pro Ala Arg Leu 425
430 435Cys Thr Pro Cys Arg Gln Gly Leu Gly Trp Met Leu Leu Leu Cys
440 445 450Pro Thr Trp Tyr Leu Val Gln Gly Cys Pro Ser Arg Cys Leu
Ile 455 460 465Asn Ser Ser Ser Leu 47020144PRTHomo
sapiensmisc_featureIncyte ID No 1932945 20Met Glu Arg Glu Gly Ser
Gly Gly Ser Gly Gly Ser Ala Gly Leu1 5 10 15Leu Gln Gln Ile Leu Ser
Leu Lys Val Val Pro Arg Val Gly Asn 20 25 30Gly Thr Leu Cys Pro Asn
Ser Thr Ser Leu Cys Ser Phe Pro Glu 35 40 45Met Trp Tyr Gly Val Phe
Leu Trp Ala Leu Val Ser Ser Leu Phe 50 55 60Phe His Val Pro Ala Gly
Leu Leu Ala Leu Phe Thr Leu Arg His 65 70 75His Lys Tyr Gly Arg Phe
Met Ser Val Ser Ile Leu Leu Met Gly 80 85 90Ile Val Gly Pro Ile Thr
Ala Gly Ile Leu Thr Ser Ala Ala Ile 95 100 105Ala Gly Val Tyr Arg
Ala Ala Gly Lys Glu Met Ile Pro Phe Glu 110 115 120Ala Leu Thr Leu
Gly Thr Gly Gln Thr Phe Cys Val Leu Val Val 125 130 135Ser Phe Leu
Arg Ile Leu Ala Thr Leu 14021221PRTHomo sapiensmisc_featureIncyte
ID No 2061026 21Met Ala Leu Ala Leu Ala Ala Leu Ala Ala Val Glu Pro
Ala Cys1 5 10 15Gly Ser Arg Tyr Gln Gln Leu Gln Asn Glu Glu Glu Ser
Gly Glu 20 25 30Pro Glu Gln Ala Ala Gly Asp Ala Pro Pro Pro Tyr Ser
Ser Ile 35 40 45Ser Ala Glu Ser Ala Ala Tyr Phe Asp Tyr Lys Asp Glu
Ser Gly 50 55 60Phe Pro Lys Pro Pro Ser Tyr Asn Val Ala Thr Thr Leu
Pro Ser 65 70 75Tyr Asp Glu Ala Glu Arg Thr Lys Ala Glu Ala Thr Ile
Pro Leu 80 85 90Val Pro Gly Arg Asp Glu Asp Phe Val Gly Arg Asp Asp
Phe Asp 95 100 105Asp Ala Asp Gln Leu Arg Ile Gly Asn Asp Gly Ile
Phe Met Leu 110 115 120Thr Phe Phe Met Ala Phe Leu Phe Asn Trp Ile
Gly Phe Phe Leu 125 130 135Ser Phe Cys Leu Thr Thr Ser Ala Ala Gly
Arg Tyr Gly Ala Ile 140 145 150Ser Gly Phe Gly Leu Ser Leu Ile Lys
Trp Ile Leu Ile Val Arg 155 160 165Phe Ser Thr Tyr Phe Pro Gly Tyr
Phe Asp Gly Gln Tyr Trp Leu 170 175 180Trp Trp Val Phe Leu Val Leu
Gly Phe Leu Leu Phe Leu Arg Gly 185 190 195Phe Ile Asn Tyr Ala Lys
Val Arg Lys Met Pro Glu Thr Phe Ser 200 205 210Asn Leu Pro Arg Thr
Arg Val Leu Phe Ile Tyr 215 22022688PRTHomo
sapiensmisc_featureIncyte ID No 2096687 22Met Ser Ala Glu Ser Gly
Pro Gly Thr Arg Leu Arg Asn Leu Pro1 5 10 15Val Met Gly Asp Gly Leu
Glu Thr Ser Gln Met Ser Thr Thr Gln 20 25 30Ala Gln Ala Gln Pro Gln
Pro Ala Asn Ala Ala Ser Thr Asn Pro 35 40 45Pro Pro Pro Glu Thr Ser
Asn Pro Asn Lys Pro Lys Arg Gln Thr 50 55 60Asn Gln Leu Gln Tyr Leu
Leu Arg Val Val Leu Lys Thr Leu Trp 65 70 75Lys His Gln Phe Ala Trp
Pro Phe Gln Gln Pro Val Asp Ala Val 80 85 90Lys Leu Asn Leu Pro Asp
Tyr Tyr Lys Ile Ile Lys Thr Pro Met 95 100 105Asp Met Gly Thr Ile
Lys Lys Arg Leu Glu Asn Asn Tyr Tyr Trp 110 115 120Asn Ala Gln Glu
Cys Ile Gln Asp Phe Asn Thr Met Phe Thr Asn 125 130 135Cys Tyr Ile
Tyr Asn Lys Pro Gly Asp Asp Ile Val Leu Met Ala 140 145 150Glu Ala
Leu Glu Lys Leu Phe Leu Gln Lys Ile Asn Glu Leu Pro 155 160 165Thr
Glu Glu Thr Glu Ile Met Ile Val Gln Ala Lys Gly Arg Gly 170 175
180Arg Gly Arg Lys Glu Thr Gly Thr Ala Lys Pro Gly Val Ser Thr 185
190 195Val Pro Asn Thr Thr Gln Ala Ser Thr Pro Pro Gln Thr Gln Thr
200 205 210Pro Gln Pro Asn Pro Pro Pro Val Gln Ala Thr Pro His Pro
Phe 215 220 225Pro Ala Val Thr Pro Asp Leu Ile Val Gln Thr Pro Val
Met Thr 230 235 240Val Val Pro Pro Gln Pro Leu Gln Thr Pro Pro Pro
Val Pro Pro 245 250 255Gln Pro Gln Pro Pro Pro Ala Pro Ala Pro Gln
Pro Val Gln Ser 260 265 270His Pro Pro Ile Ile Ala Ala Thr Pro Gln
Pro Val Lys Thr Lys 275 280 285Lys Gly Val Lys Arg Lys Ala Asp Thr
Thr Thr Pro Thr Thr Ile 290 295 300Asp Pro Ile His Glu Pro Pro Ser
Leu Pro Pro Glu Pro Lys Thr 305 310 315Thr Lys Leu Gly Gln Arg Arg
Glu Ser Ser Arg Pro Val Lys Pro 320 325 330Pro Lys Lys Asp Val Pro
Asp Ser Gln Gln His Pro Ala Pro Glu 335 340 345Lys Ser Ser Lys Val
Ser Glu Gln Leu Lys Cys Cys Ser Gly Ile 350 355 360Leu Lys Glu Met
Phe Ala Lys Lys His Ala Ala Tyr Ala Trp Pro 365 370 375Phe Tyr Lys
Pro Val Asp Val Glu Ala Leu Gly Leu His Asp Tyr 380 385 390Cys Asp
Ile Ile Lys His Pro Met Asp Met Ser Thr Ile Lys Ser 395 400 405Lys
Leu Glu Ala Arg Glu Tyr Arg Asp Ala Gln Glu Phe Gly Ala 410 415
420Asp Val Arg Leu Met Phe Ser Asn Cys Tyr Lys Tyr Asn Pro Pro 425
430 435Asp His Glu Val Val Ala Met Ala Arg Lys Leu Gln Asp Val
Phe
440 445 450Glu Met Arg Phe Ala Lys Met Pro Asp Glu Pro Glu Glu Pro
Val 455 460 465Val Ala Val Ser Ser Pro Ala Val Pro Pro Pro Thr Lys
Val Val 470 475 480Ala Pro Pro Ser Ser Ser Asp Ser Ser Ser Asp Ser
Ser Ser Asp 485 490 495Ser Asp Ser Ser Thr Asp Asp Ser Glu Glu Glu
Arg Ala Gln Arg 500 505 510Leu Ala Glu Leu Gln Glu Gln Leu Lys Ala
Val His Glu Gln Leu 515 520 525Ala Ala Leu Ser Gln Pro Gln Gln Asn
Lys Pro Lys Lys Lys Glu 530 535 540Lys Asp Lys Lys Glu Lys Lys Lys
Glu Lys His Lys Arg Lys Glu 545 550 555Glu Val Glu Glu Asn Lys Lys
Ser Lys Ala Lys Glu Pro Pro Pro 560 565 570Lys Lys Thr Lys Lys Asn
Asn Ser Ser Asn Ser Asn Val Ser Lys 575 580 585Lys Glu Pro Ala Pro
Met Lys Ser Lys Pro Pro Pro Thr Tyr Glu 590 595 600Ser Glu Glu Glu
Asp Lys Cys Lys Pro Met Ser Tyr Glu Glu Lys 605 610 615Arg Gln Leu
Ser Leu Asp Ile Asn Lys Leu Pro Gly Glu Lys Leu 620 625 630Gly Arg
Val Val His Ile Ile Gln Ser Arg Glu Pro Ser Leu Lys 635 640 645Asn
Ser Asn Pro Asp Glu Ile Glu Ile Asp Phe Glu Thr Leu Lys 650 655
660Pro Ser Thr Leu Arg Glu Leu Gly Ala Leu Cys His Leu Leu Phe 665
670 675Ala Glu Glu Lys Glu Thr Phe Lys Leu Arg Lys Leu Met 680
68523439PRTHomo sapiensmisc_featureIncyte ID No 2100530 23Met Gly
Ser Gln Glu Val Leu Gly His Ala Ala Arg Leu Ala Ser1 5 10 15Ser Gly
Leu Leu Leu Gln Val Leu Phe Arg Leu Ile Thr Phe Val 20 25 30Leu Asn
Ala Phe Ile Leu Arg Phe Leu Ser Lys Glu Ile Val Gly 35 40 45Val Val
Asn Val Arg Leu Thr Leu Leu Tyr Ser Thr Thr Leu Phe 50 55 60Leu Ala
Arg Glu Ala Phe Arg Arg Ala Cys Leu Ser Gly Gly Thr 65 70 75Gln Arg
Asp Trp Ser Gln Thr Leu Asn Leu Leu Trp Leu Thr Val 80 85 90Pro Leu
Gly Val Phe Trp Ser Leu Phe Leu Gly Trp Ile Trp Leu 95 100 105Gln
Leu Leu Glu Val Pro Asp Pro Asn Val Val Pro His Tyr Ala 110 115
120Thr Gly Val Val Leu Phe Gly Leu Ser Ala Val Val Glu Leu Leu 125
130 135Gly Glu Pro Phe Trp Val Leu Ala Gln Ala His Met Phe Val Lys
140 145 150Leu Lys Val Ile Ala Glu Ser Leu Ser Val Ile Leu Lys Ser
Val 155 160 165Leu Thr Ala Phe Leu Val Leu Trp Leu Pro His Trp Gly
Leu Tyr 170 175 180Ile Phe Ser Leu Ala Gln Leu Phe Tyr Thr Thr Val
Leu Val Leu 185 190 195Cys Tyr Val Ile Tyr Phe Thr Lys Leu Leu Gly
Ser Pro Glu Ser 200 205 210Thr Lys Leu Gln Thr Leu Pro Val Ser Arg
Ile Thr Asp Leu Leu 215 220 225Pro Asn Ile Thr Arg Asn Gly Ala Phe
Ile Asn Trp Lys Glu Ala 230 235 240Lys Leu Thr Trp Ser Phe Phe Lys
Gln Ser Phe Leu Lys Gln Ile 245 250 255Leu Thr Glu Gly Glu Arg Tyr
Val Met Thr Phe Leu Asn Val Leu 260 265 270Asn Phe Gly Asp Gln Gly
Val Tyr Asp Ile Val Asn Asn Leu Gly 275 280 285Ser Leu Val Ala Arg
Leu Ile Phe Gln Pro Ile Glu Glu Ser Phe 290 295 300Tyr Ile Phe Phe
Ala Lys Val Leu Glu Arg Gly Lys Asp Ala Thr 305 310 315Leu Gln Lys
Gln Glu Asp Val Ala Val Ala Ala Ala Val Leu Glu 320 325 330Ser Leu
Leu Lys Leu Ala Leu Leu Ala Gly Leu Thr Ile Thr Val 335 340 345Phe
Gly Phe Ala Tyr Ser Gln Leu Ala Leu Asp Ile Tyr Gly Gly 350 355
360Thr Met Leu Ser Ser Gly Ser Gly Pro Val Leu Leu Arg Ser Tyr 365
370 375Cys Leu Tyr Val Leu Leu Leu Ala Ile Asn Gly Val Thr Glu Cys
380 385 390Phe Thr Phe Ala Ala Met Ser Lys Glu Glu Val Asp Arg Tyr
Ser 395 400 405Ser Ala Val Ser Arg Ala Gly Gln Pro Asp Trp His Thr
Leu Leu 410 415 420Trp Gly Pro Ser Val Trp Glu Gln Leu Ser Gly Gln
His Xaa Ser 425 430 435Gln Arg Pro Ser24192PRTHomo
sapiensmisc_featureIncyte ID No 2357636 24Met Thr Ala Val Gly Val
Gln Ala Gln Arg Pro Leu Gly Gln Arg1 5 10 15Gln Pro Arg Arg Ser Phe
Phe Glu Ser Phe Ile Arg Thr Leu Ile 20 25 30Ile Thr Cys Val Ala Leu
Ala Val Val Leu Ser Ser Val Ser Ile 35 40 45Cys Asp Gly His Trp Leu
Leu Ala Glu Asp Arg Leu Phe Gly Leu 50 55 60Trp His Phe Cys Thr Thr
Thr Asn Gln Ser Val Pro Ile Cys Phe 65 70 75Arg Asp Leu Gly Gln Ala
His Val Pro Gly Leu Ala Val Gly Met 80 85 90Gly Leu Val Arg Ser Val
Gly Ala Leu Ala Val Val Ala Ala Ile 95 100 105Phe Gly Leu Glu Phe
Leu Met Val Ser Gln Leu Cys Glu Asp Lys 110 115 120His Ser Gln Cys
Lys Trp Val Met Gly Ser Ile Leu Leu Leu Val 125 130 135Ser Phe Val
Leu Ser Ser Gly Gly Leu Leu Gly Phe Val Ile Leu 140 145 150Leu Arg
Asn Gln Val Thr Leu Ile Gly Phe Thr Leu Met Phe Trp 155 160 165Cys
Glu Phe Thr Ala Ser Phe Leu Leu Phe Leu Asn Ala Ile Ser 170 175
180Gly Leu His Ile Asn Ser Ile Thr His Pro Trp Glu 185
19025175PRTHomo sapiensmisc_featureIncyte ID No 2365230 25Met Lys
Glu Val Thr Arg Thr Trp Lys Ile Val Gly Gly Val Thr1 5 10 15His Ala
Asn Ser Tyr Tyr Lys Asn Gly Trp Ile Val Met Ile Ala 20 25 30Ile Gly
Trp Ala Arg Gly Ala Gly Gly Thr Ile Ile Thr Asn Phe 35 40 45Glu Arg
Leu Val Lys Gly Asp Trp Lys Pro Glu Gly Asp Glu Trp 50 55 60Leu Lys
Met Ser Tyr Pro Ala Lys Val Thr Leu Leu Gly Ser Val 65 70 75Ile Phe
Thr Phe Gln His Thr Gln His Leu Ala Ile Ser Lys His 80 85 90Asn Leu
Met Phe Leu Tyr Thr Ile Phe Ile Val Ala Thr Lys Ile 95 100 105Thr
Met Met Thr Thr Gln Thr Ser Thr Met Thr Phe Ala Pro Phe 110 115
120Glu Asp Thr Leu Ser Trp Met Leu Phe Gly Trp Gln Gln Pro Phe 125
130 135Ser Ser Cys Glu Lys Lys Ser Glu Ala Lys Ser Pro Ser Asn Gly
140 145 150Val Gly Ser Leu Ala Ser Lys Pro Val Asp Val Ala Ser Asp
Asn 155 160 165Val Lys Lys Lys His Thr Lys Lys Asn Glu 170
1752691PRTHomo sapiensmisc_featureIncyte ID No 2455121 26Met Tyr
Pro Pro Pro Pro Pro Pro Pro His Arg Asp Phe Ile Ser1 5 10 15Val Thr
Leu Ser Phe Gly Glu Ser Tyr Asp Asn Ser Lys Ser Trp 20 25 30Arg Arg
Arg Ser Cys Trp Arg Lys Trp Lys Gln Leu Ser Arg Leu 35 40 45Gln Arg
Asn Met Ile Leu Phe Leu Leu Ala Phe Leu Leu Phe Cys 50 55 60Gly Leu
Leu Phe Tyr Ile Asn Leu Ala Asp His Trp Lys Ala Leu 65 70 75Ala Phe
Arg Leu Gly Glu Glu Gln Lys Met Arg Pro Glu Ile Ala 80 85
90Gly27214PRTHomo sapiensmisc_featureIncyte ID No 2472514 27Met Gln
Pro Thr Ser Trp Ala Val Ser Cys Gly Leu Arg Pro Leu1 5 10 15Pro Ser
Trp Lys Pro Gln Gly Gly Glu Gly Arg Gly Gly Glu Glu 20 25 30Arg Arg
Gly Thr Val Met Gly Pro Trp Ser Arg Val Arg Val Ala 35 40 45Lys Cys
Gln Met Leu Val Thr Cys Phe Phe Ile Leu Leu Leu Gly 50 55 60Leu Ser
Val Ala Thr Met Val Thr Leu Thr Tyr Phe Gly Ala His 65 70 75Phe Ala
Val Ile Arg Arg Ala Ser Leu Glu Lys Asn Pro Tyr Gln 80 85 90Ala Val
His Gln Trp Ala Phe Ser Ala Gly Leu Ser Leu Val Gly 95 100 105Leu
Leu Thr Leu Gly Ala Val Leu Ser Ala Ala Ala Thr Val Arg 110 115
120Glu Ala Gln Gly Leu Met Ala Gly Gly Phe Leu Cys Phe Ser Leu 125
130 135Ala Phe Cys Ala Gln Val Gln Val Val Phe Trp Arg Leu His Ser
140 145 150Pro Thr Gln Val Glu Asp Ala Met Leu Asp Thr Tyr Asp Leu
Val 155 160 165Tyr Glu Gln Ala Met Lys Gly Thr Ser His Val Arg Arg
Gln Glu 170 175 180Leu Ala Ala Ile Gln Asp Val Val Ser Val Gly Thr
Ala Gly Trp 185 190 195Gln Gly Gly Gln Leu Leu Leu Gly Leu Gln Phe
Arg Glu Gln Ala 200 205 210Gln Gly Gly Gln 28250PRTHomo
sapiensmisc_featureIncyte ID No 2543486 28Met Ser Val Ile Phe Phe
Ala Cys Val Val Arg Val Arg Asp Gly1 5 10 15Leu Pro Leu Ser Ala Ser
Thr Asp Phe Tyr His Thr Gln Asp Phe 20 25 30Leu Glu Trp Arg Arg Arg
Leu Lys Ser Leu Ala Leu Arg Leu Ala 35 40 45Gln Tyr Pro Gly Arg Gly
Ser Ala Glu Gly Cys Asp Phe Ser Ile 50 55 60His Phe Ser Ser Phe Gly
Asp Val Ala Cys Met Ala Ile Cys Ser 65 70 75Cys Gln Cys Pro Ala Ala
Met Ala Phe Cys Phe Leu Glu Thr Leu 80 85 90Trp Trp Glu Phe Thr Ala
Ser Tyr Asp Thr Thr Cys Ile Gly Leu 95 100 105Ala Ser Arg Pro Tyr
Ala Phe Leu Glu Phe Asp Ser Ile Ile Gln 110 115 120Lys Val Lys Trp
His Phe Asn Tyr Val Ser Ser Ser Gln Met Glu 125 130 135Cys Ser Leu
Glu Lys Ile Gln Glu Glu Leu Lys Leu Gln Pro Pro 140 145 150Ala Val
Leu Thr Leu Glu Asp Thr Asp Val Ala Asn Gly Val Met 155 160 165Asn
Gly His Thr Pro Met His Leu Glu Pro Ala Pro Asn Phe Arg 170 175
180Met Glu Pro Val Thr Ala Leu Gly Ile Leu Ser Leu Ile Leu Asn 185
190 195Ile Met Cys Ala Ala Leu Asn Leu Ile Arg Gly Val His Leu Ala
200 205 210Glu His Ser Leu Gln Val Ala His Glu Glu Ile Gly Asn Ile
Leu 215 220 225Ala Phe Leu Val Pro Phe Val Ala Cys Ile Phe Gln Asp
Pro Arg 230 235 240Ser Trp Phe Cys Trp Leu Asp Gln Thr Ser 245
2502984PRTHomo sapiensmisc_featureIncyte ID No 2778171 29Met Ala
Thr Gly Thr Asp Gln Val Val Gly Leu Gly Leu Val Ala1 5 10 15Val Ser
Leu Ile Ile Phe Thr Tyr Tyr Thr Ala Trp Val Ile Leu 20 25 30Leu Pro
Phe Ile Asp Ser Gln His Val Ile His Lys Tyr Phe Leu 35 40 45Pro Arg
Ala Tyr Ala Val Ala Ile Pro Leu Ala Ala Gly Leu Leu 50 55 60Leu Leu
Leu Phe Val Gly Leu Phe Ile Ser Tyr Val Met Leu Lys 65 70 75Ser Lys
Arg Val Thr Lys Lys Ala Gln 8030277PRTHomo
sapiensmisc_featureIncyte ID No 2799575 30Met Ala Ser Ala Glu Leu
Asp Tyr Thr Ile Glu Ile Pro Asp Gln1 5 10 15Pro Cys Trp Ser Gln Lys
Asn Ser Pro Ser Pro Gly Gly Lys Glu 20 25 30Ala Glu Thr Arg Gln Pro
Val Val Ile Leu Leu Gly Trp Gly Gly 35 40 45Cys Lys Asp Lys Asn Leu
Ala Lys Tyr Ser Ala Ile Tyr His Lys 50 55 60Arg Gly Cys Ile Val Ile
Arg Tyr Thr Ala Pro Trp His Met Val 65 70 75Phe Phe Ser Glu Ser Leu
Gly Ile Pro Ser Leu Arg Val Leu Ala 80 85 90Gln Lys Leu Leu Glu Leu
Leu Phe Asp Tyr Glu Ile Glu Lys Glu 95 100 105Pro Leu Leu Phe His
Val Phe Ser Asn Gly Gly Val Met Leu Tyr 110 115 120Arg Tyr Val Leu
Glu Leu Leu Gln Thr Arg Arg Phe Cys Arg Leu 125 130 135Arg Val Val
Gly Thr Ile Phe Asp Ser Ala Pro Gly Asp Ser Asn 140 145 150Leu Val
Gly Ala Leu Arg Ala Leu Ala Ala Ile Leu Glu Arg Arg 155 160 165Ala
Ala Met Leu Arg Leu Leu Leu Leu Val Ala Phe Ala Leu Val 170 175
180Val Val Leu Phe His Val Leu Leu Ala Pro Ile Thr Ala Leu Phe 185
190 195His Thr His Phe Tyr Asp Arg Leu Gln Asp Ala Gly Ser Arg Trp
200 205 210Pro Glu Leu Tyr Leu Tyr Ser Arg Ala Asp Glu Val Val Leu
Ala 215 220 225Arg Asp Ile Glu Arg Met Val Glu Ala Arg Leu Ala Arg
Arg Val 230 235 240Leu Ala Arg Ser Val Asp Phe Val Ser Ser Ala His
Val Ser His 245 250 255Leu Arg Asp Tyr Pro Thr Tyr Tyr Thr Ser Leu
Cys Val Asp Phe 260 265 270Met Arg Asn Cys Val Arg Cys
27531273PRTHomo sapiensmisc_featureIncyte ID No 2804955 31Met Ser
Gly Ser Gln Ser Glu Val Ala Pro Ser Pro Gln Ser Pro1 5 10 15Arg Ser
Pro Glu Met Gly Arg Asp Leu Arg Pro Gly Ser Arg Val 20 25 30Leu Leu
Leu Leu Leu Leu Leu Leu Leu Val Tyr Leu Thr Gln Pro 35 40 45Gly Asn
Gly Asn Glu Gly Ser Val Thr Gly Ser Cys Tyr Cys Gly 50 55 60Lys Arg
Ile Ser Ser Asp Ser Pro Pro Ser Val Gln Phe Met Asn 65 70 75Arg Leu
Arg Lys His Leu Arg Ala Tyr His Arg Cys Leu Tyr Tyr 80 85 90Thr Arg
Phe Gln Leu Leu Ser Trp Ser Val Cys Gly Gly Asn Lys 95 100 105Asp
Pro Trp Val Gln Glu Leu Met Ser Cys Leu Asp Leu Lys Glu 110 115
120Cys Gly His Ala Tyr Ser Gly Ile Val Ala His Gln Lys His Leu 125
130 135Leu Pro Thr Ser Pro Pro Ile Ser Gln Ala Ser Glu Gly Ala Ser
140 145 150Ser Asp Ile His Thr Pro Ala Gln Met Leu Leu Ser Thr Leu
Gln 155 160 165Ser Thr Gln Arg Pro Thr Leu Pro Val Gly Ser Leu Ser
Ser Asp 170 175 180Lys Glu Leu Thr Arg Pro Asn Glu Thr Thr Ile His
Thr Ala Gly 185 190 195His Ser Leu Ala Ala Gly Pro Glu Ala Gly Glu
Asn Gln Lys Gln 200 205 210Pro Glu Lys Asn Ala Gly Pro Thr Ala Arg
Thr Ser Ala Thr Val 215 220 225Pro Val Leu Cys Leu Leu Ala Ile Ile
Phe Ile Leu Thr Ala Ala 230 235 240Leu Ser Tyr Val Leu Cys Lys Arg
Arg Arg Gly Gln Ser Pro Gln 245 250 255Ser Ser Pro Asp Leu Pro Val
His Tyr Ile Pro Val Ala Pro Asp 260 265 270Ser Asn Thr 32524PRTHomo
sapiensmisc_featureIncyte ID No 2806395 32Met Ser Gln Gly Ser Pro
Gly Asp Trp Ala Pro Leu Asp Pro Thr1 5 10 15Pro Gly Pro Pro Ala Ser
Pro Asn Pro Phe Val His Glu Leu His 20 25 30Leu Ser Arg Leu Gln
Arg Val Lys Phe Cys Leu Leu Gly Ala Leu 35 40 45Leu Ala Pro Ile Arg
Val Leu Leu Ala Phe Ile Val Leu Phe Leu 50 55 60Leu Trp Pro Phe Ala
Trp Leu Gln Val Ala Gly Leu Ser Glu Glu 65 70 75Gln Leu Gln Glu Pro
Ile Thr Gly Trp Arg Lys Thr Val Cys His 80 85 90Asn Gly Val Leu Gly
Leu Ser Arg Leu Leu Phe Phe Leu Leu Gly 95 100 105Phe Leu Arg Ile
Arg Val Arg Gly Gln Arg Ala Ser Arg Leu Gln 110 115 120Ala Pro Val
Leu Val Ala Ala Pro His Ser Thr Phe Phe Asp Pro 125 130 135Ile Val
Leu Leu Pro Cys Asp Leu Pro Lys Val Val Ser Arg Ala 140 145 150Glu
Asn Leu Ser Val Pro Val Ile Gly Ala Leu Leu Arg Phe Asn 155 160
165Gln Ala Ile Leu Val Ser Arg His Asp Pro Ala Ser Arg Arg Arg 170
175 180Val Val Glu Glu Val Arg Arg Arg Ala Thr Ser Gly Gly Lys Trp
185 190 195Pro Gln Val Leu Phe Phe Pro Glu Gly Thr Cys Ser Asn Lys
Lys 200 205 210Ala Leu Leu Lys Phe Lys Pro Gly Ala Phe Ile Ala Gly
Val Pro 215 220 225Val Gln Pro Val Leu Ile Arg Tyr Pro Asn Ser Leu
Asp Thr Thr 230 235 240Ser Trp Ala Trp Arg Gly Pro Gly Val Leu Lys
Val Leu Trp Leu 245 250 255Thr Ala Ser Gln Pro Cys Ser Ile Val Asp
Val Glu Phe Leu Pro 260 265 270Val Tyr His Pro Ser Pro Glu Glu Ser
Arg Asp Pro Thr Leu Tyr 275 280 285Ala Asn Asn Val Gln Arg Val Met
Ala Gln Ala Leu Gly Ile Pro 290 295 300Ala Thr Glu Cys Glu Phe Val
Gly Ser Leu Pro Val Ile Val Val 305 310 315Gly Arg Leu Lys Val Ala
Leu Glu Pro Gln Leu Trp Glu Leu Gly 320 325 330Lys Val Leu Arg Lys
Ala Gly Leu Ser Ala Gly Tyr Val Asp Ala 335 340 345Gly Ala Glu Pro
Gly Arg Ser Arg Met Ile Ser Gln Glu Glu Phe 350 355 360Ala Arg Gln
Leu Gln Leu Ser Asp Pro Gln Thr Val Ala Gly Ala 365 370 375Phe Gly
Tyr Phe Gln Gln Asp Thr Lys Gly Leu Val Asp Phe Arg 380 385 390Asp
Val Ala Leu Ala Leu Ala Ala Leu Asp Gly Gly Arg Ser Leu 395 400
405Glu Glu Leu Thr Arg Leu Ala Phe Glu Leu Phe Ala Glu Glu Gln 410
415 420Ala Glu Gly Pro Asn Arg Leu Leu Tyr Lys Asp Gly Phe Ser Thr
425 430 435Ile Leu His Leu Leu Leu Gly Ser Pro His Pro Ala Ala Thr
Ala 440 445 450Leu His Ala Glu Leu Cys Gln Ala Gly Ser Ser Gln Gly
Leu Ser 455 460 465Leu Cys Gln Phe Gln Asn Phe Ser Leu His Asp Pro
Leu Tyr Gly 470 475 480Lys Leu Phe Ser Thr Tyr Leu Arg Pro Pro His
Thr Ser Arg Gly 485 490 495Thr Ser Gln Thr Pro Asn Ala Ser Ser Pro
Gly Asn Pro Thr Ala 500 505 510Leu Ala Asn Gly Thr Val Gln Ala Pro
Lys Gln Lys Gly Asp 515 52033257PRTHomo sapiensmisc_featureIncyte
ID No 2836858 33Met Asp Phe Ser Arg Leu His Met Tyr Ser Pro Pro Gln
Cys Val1 5 10 15Pro Glu Asn Thr Gly Tyr Thr Tyr Ala Leu Ser Ser Ser
Tyr Ser 20 25 30Ser Asp Ala Leu Asp Phe Glu Thr Glu His Lys Leu Asp
Pro Val 35 40 45Phe Asp Ser Pro Arg Met Ser Arg Arg Ser Leu Arg Leu
Ala Thr 50 55 60Thr Ala Cys Thr Leu Gly Asp Gly Glu Ala Val Gly Ala
Asp Ser 65 70 75Gly Thr Ser Ser Ala Val Ser Leu Lys Asn Arg Ala Ala
Arg Thr 80 85 90Thr Lys Gln Arg Arg Ser Thr Asn Lys Ser Ala Phe Ser
Ile Asn 95 100 105His Val Ser Arg Gln Val Thr Ser Ser Gly Val Ser
His Gly Gly 110 115 120Thr Val Ser Leu Gln Asp Ala Val Thr Arg Arg
Pro Pro Val Leu 125 130 135Asp Glu Ser Trp Ile Arg Glu Gln Thr Thr
Val Asp His Phe Trp 140 145 150Gly Leu Asp Asp Asp Gly Asp Leu Lys
Gly Gly Asn Lys Ala Ala 155 160 165Ile Gln Gly Asn Gly Asp Val Gly
Ala Ala Ala Ala Thr Ala His 170 175 180Asn Gly Phe Ser Cys Ser Asn
Cys Ser Met Leu Ser Glu Arg Lys 185 190 195Asp Val Leu Thr Ala His
Pro Ala Ala Pro Gly Pro Val Ser Arg 200 205 210Val Tyr Ser Arg Asp
Arg Asn Gln Lys Cys Lys Ser Gln Ser Phe 215 220 225Lys Thr Gln Lys
Lys Val Cys Phe Pro Asn Leu Ile Phe Pro Phe 230 235 240Cys Lys Ser
Gln Cys Leu His Tyr Leu Ser Trp Arg Leu Lys Ile 245 250 255Ile
Pro34274PRTHomo sapiensmisc_featureIncyte ID No 2844513 34Met Arg
Ala Ala Gly Val Gly Leu Val Asp Cys His Cys His Leu1 5 10 15Ser Ala
Pro Asp Phe Asp Arg Asp Leu Asp Asp Val Leu Glu Lys 20 25 30Ala Lys
Lys Ala Asn Val Val Ala Leu Val Ala Val Ala Glu His 35 40 45Ser Gly
Glu Phe Glu Lys Ile Met Gln Leu Ser Glu Arg Tyr Asn 50 55 60Gly Phe
Val Leu Pro Cys Leu Gly Val His Pro Val Gln Gly Leu 65 70 75Pro Pro
Glu Asp Gln Arg Ser Val Thr Leu Lys Asp Leu Asp Val 80 85 90Ala Leu
Pro Ile Ile Glu Asn Tyr Lys Asp Arg Leu Leu Ala Ile 95 100 105Gly
Glu Val Gly Leu Asp Phe Ser Pro Arg Phe Ala Gly Thr Gly 110 115
120Glu Gln Lys Glu Glu Gln Arg Gln Val Leu Ile Arg Gln Ile Gln 125
130 135Leu Ala Lys Arg Leu Asn Leu Pro Val Asn Val His Ser Arg Ser
140 145 150Ala Gly Arg Pro Thr Ile Asn Leu Leu Gln Glu Gln Gly Ala
Glu 155 160 165Lys Val Leu Leu His Ala Phe Asp Gly Arg Pro Ser Val
Ala Met 170 175 180Glu Gly Val Arg Ala Gly Tyr Phe Phe Ser Ile Pro
Pro Ser Ile 185 190 195Ile Arg Ser Gly Gln Lys Gln Lys Leu Val Lys
Gln Leu Pro Leu 200 205 210Thr Ser Ile Cys Leu Glu Thr Asp Ser Pro
Ala Leu Gly Pro Glu 215 220 225Lys Gln Val Arg Asn Glu Pro Trp Asn
Ile Ser Ile Ser Ala Glu 230 235 240Tyr Ile Ala Gln Val Lys Gly Ile
Ser Val Glu Glu Val Ile Glu 245 250 255Val Thr Thr Gln Asn Ala Leu
Lys Leu Phe Pro Lys Leu Arg His 260 265 270Leu Leu Gln Lys
35281PRTHomo sapiensmisc_featureIncyte ID No 3000380 35Met Ser Glu
Pro Gln Pro Asp Leu Glu Pro Pro Gln His Gly Leu1 5 10 15Tyr Met Leu
Phe Leu Leu Val Leu Val Phe Phe Leu Met Gly Leu 20 25 30Val Gly Phe
Met Ile Cys His Val Leu Lys Lys Lys Gly Tyr Arg 35 40 45Cys Arg Thr
Ser Arg Gly Ser Glu Pro Asp Asp Ala Gln Leu Gln 50 55 60Pro Pro Glu
Asp Asp Asp Met Asn Glu Asp Thr Val Glu Arg Ile 65 70 75Val Arg Cys
Ile Ile Gln Asn Glu Val Trp Met Pro Pro Pro Ala 80 85 90Cys Arg Thr
Glu Pro Pro Pro Ile Ile Thr Gln Cys Thr Trp Ala 95 100 105Leu Gln
Pro Leu Ala Val His Cys Ser Arg Ser Lys Arg Pro Pro 110 115 120Leu
Val Arg Gln Gly Arg Ser Lys Glu Gly Lys Ser Arg Pro Arg 125 130
135Thr Gly Glu Thr Thr Val Phe Ser Val Gly Arg Phe Arg Val Thr 140
145 150His Ile Glu Lys Arg Tyr Gly Leu His Glu His Arg Asp Gly Ser
155 160 165Pro Thr Asp Arg Ser Trp Gly Ser Arg Gly Gly Gln Asp Pro
Gly 170 175 180Gly Gly Gln Gly Ser Gly Gly Gly His Pro Lys Ala Gly
Met Leu 185 190 195Pro Trp Arg Gly Cys Pro Pro Glu Arg Pro Gln Pro
Gln Val Leu 200 205 210Ala Ser Pro Pro Val Gln Asn Gly Gly Leu Arg
Asp Ser Ser Leu 215 220 225Thr Pro Arg Ala Leu Glu Gly Asn Pro Arg
Ala Ser Ala Glu Pro 230 235 240Thr Leu Arg Ala Gly Gly Arg Gly Pro
Ser Pro Gly Leu Pro Thr 245 250 255Gln Glu Ala Asn Gly Gln Pro Ser
Lys Pro Asp Thr Ser Asp His 260 265 270Gln Val Ser Leu Pro Gln Gly
Ala Gly Ser Met 275 28036335PRTHomo sapiensmisc_featureIncyte ID No
182532 36Met Gly Pro Leu Ser Ala Pro Pro Cys Thr His Leu Ile Thr
Trp1 5 10 15Lys Gly Val Leu Leu Thr Ala Ser Leu Leu Asn Phe Trp Asn
Pro 20 25 30Pro Thr Thr Ala Gln Val Thr Ile Glu Ala Gln Pro Pro Lys
Val 35 40 45Ser Glu Gly Lys Asp Val Leu Leu Leu Val His Asn Leu Pro
Gln 50 55 60Asn Leu Ala Gly Tyr Ile Trp Tyr Lys Gly Gln Met Thr Tyr
Val 65 70 75Tyr His Tyr Ile Ile Ser Tyr Ile Val Asp Gly Lys Ile Ile
Ile 80 85 90Tyr Gly Pro Ala Tyr Ser Gly Arg Glu Arg Val Tyr Ser Asn
Ala 95 100 105Ser Leu Leu Ile Gln Asn Val Thr Gln Glu Asp Ala Gly
Ser Tyr 110 115 120Thr Leu His Ile Ile Lys Arg Gly Asp Gly Thr Arg
Gly Glu Thr 125 130 135Gly His Phe Thr Phe Thr Leu Tyr Leu Glu Thr
Pro Lys Pro Ser 140 145 150Ile Ser Ser Ser Asn Leu Tyr Pro Arg Glu
Asp Met Glu Ala Val 155 160 165Ser Leu Thr Cys Asp Pro Glu Thr Pro
Asp Ala Ser Tyr Leu Trp 170 175 180Trp Met Asn Gly Gln Ser Leu Pro
Met Thr His Ser Leu Gln Leu 185 190 195Ser Lys Asn Lys Arg Thr Leu
Phe Leu Phe Gly Val Thr Lys Tyr 200 205 210Thr Ala Gly Pro Tyr Glu
Cys Glu Ile Arg Asn Pro Val Ser Gly 215 220 225Ile Arg Ser Asp Pro
Val Thr Leu Asn Val Leu Tyr Gly Pro Asp 230 235 240Leu Pro Ser Ile
Tyr Pro Ser Phe Thr Tyr Tyr Arg Ser Gly Glu 245 250 255Asn Leu Tyr
Leu Ser Cys Phe Ala Glu Ser Asn Pro Arg Ala Gln 260 265 270Tyr Ser
Trp Thr Ile Asn Gly Lys Phe Gln Leu Ser Gly Gln Lys 275 280 285Leu
Phe Ile Pro Gln Ile Thr Thr Lys His Ser Gly Leu Tyr Ala 290 295
300Cys Ser Val Arg Asn Ser Ala Thr Gly Met Glu Ser Ser Lys Ser 305
310 315Met Thr Val Lys Val Ser Ala Pro Ser Gly Thr Gly His Leu Pro
320 325 330Gly Leu Asn Pro Leu 33537280PRTHomo
sapiensmisc_featureIncyte ID No 239589 37Met Asp Leu Gln Gly Arg
Gly Val Pro Ser Ile Asp Arg Leu Arg1 5 10 15Val Leu Leu Met Leu Phe
His Thr Met Ala Gln Ile Met Ala Glu 20 25 30Gln Glu Val Glu Asn Leu
Ser Gly Leu Ser Thr Asn Pro Glu Lys 35 40 45Asp Ile Phe Val Val Arg
Glu Asn Gly Thr Thr Cys Leu Met Ala 50 55 60Glu Phe Ala Ala Lys Phe
Ile Val Pro Tyr Asp Val Trp Ala Ser 65 70 75Asn Tyr Val Asp Leu Ile
Thr Glu Gln Ala Asp Ile Ala Leu Thr 80 85 90Arg Gly Ala Glu Val Lys
Gly Arg Cys Gly His Ser Gln Ser Glu 95 100 105Leu Gln Val Phe Trp
Val Asp Arg Ala Tyr Ala Leu Lys Met Leu 110 115 120Phe Val Lys Glu
Ser His Asn Met Ser Lys Gly Pro Glu Ala Thr 125 130 135Trp Arg Leu
Ser Lys Val Gln Phe Val Tyr Asp Ser Ser Glu Lys 140 145 150Thr His
Phe Lys Asp Ala Val Ser Ala Gly Lys His Thr Ala Asn 155 160 165Ser
His His Leu Ser Ala Leu Val Thr Pro Ala Gly Lys Ser Tyr 170 175
180Glu Cys Gln Ala Gln Gln Thr Ile Ser Leu Ala Ser Ser Asp Pro 185
190 195Gln Lys Thr Val Thr Met Ile Leu Ser Ala Val His Ile Gln Pro
200 205 210Phe Asp Ile Ile Ser Asp Phe Val Phe Ser Glu Glu His Lys
Cys 215 220 225Pro Val Asp Glu Arg Glu Gln Leu Glu Glu Thr Leu Pro
Leu Ile 230 235 240Leu Gly Leu Ile Leu Gly Leu Val Ile Met Val Thr
Leu Ala Ile 245 250 255Tyr His Val His His Lys Met Thr Ala Asn Gln
Val Gln Ile Pro 260 265 270Arg Asp Arg Ser Gln Tyr Lys His Met Gly
275 28038210PRTHomo sapiensmisc_featureIncyte ID No 1671302 38Met
Ser Arg Met Phe Cys Gln Ala Ala Arg Val Asp Leu Thr Leu1 5 10 15Asp
Pro Asp Thr Ala His Pro Ala Leu Met Leu Ser Pro Asp Arg 20 25 30Arg
Gly Val Arg Leu Ala Glu Arg Arg Gln Glu Val Ala Asp His 35 40 45Pro
Lys Arg Phe Ser Ala Asp Cys Cys Val Leu Gly Ala Gln Gly 50 55 60Phe
Arg Ser Gly Arg His Tyr Trp Glu Val Glu Val Gly Gly Arg 65 70 75Arg
Gly Trp Ala Val Gly Ala Ala Arg Glu Ser Thr His His Lys 80 85 90Glu
Lys Val Gly Pro Gly Gly Ser Ser Val Gly Ser Gly Asp Ala 95 100
105Ser Ser Ser Arg His His His Arg Arg Arg Arg Leu His Leu Pro 110
115 120Gln Gln Pro Leu Leu Gln Arg Glu Val Trp Cys Val Gly Thr Asn
125 130 135Gly Lys Arg Tyr Gln Ala Gln Ser Ser Thr Glu Gln Thr Leu
Leu 140 145 150Ser Pro Ser Glu Lys Pro Arg Arg Phe Gly Val Tyr Leu
Asp Tyr 155 160 165Glu Ala Gly Arg Leu Gly Phe Tyr Asn Ala Glu Thr
Leu Ala His 170 175 180Val His Thr Phe Ser Ala Ala Phe Leu Gly Glu
Arg Val Phe Pro 185 190 195Phe Phe Arg Val Leu Ser Lys Gly Thr Arg
Ile Lys Leu Cys Pro 200 205 21039279PRTHomo
sapiensmisc_featureIncyte ID No 2041858 39Met Glu Ala Val Val Asn
Leu Tyr Gln Glu Val Met Lys His Ala1 5 10 15Asp Pro Arg Ile Gln Gly
Tyr Pro Leu Met Gly Ser Pro Leu Leu 20 25 30Met Thr Ser Ile Leu Leu
Thr Tyr Val Tyr Phe Val Leu Ser Leu 35 40 45Gly Pro Arg Ile Met Ala
Asn Arg Lys Pro Phe Gln Leu Arg Gly 50 55 60Phe Met Ile Val Tyr Asn
Phe Ser Leu Val Ala Leu Ser Leu Tyr 65 70 75Ile Val Tyr Glu Phe Leu
Met Ser Gly Trp Leu Ser Thr Tyr Thr 80 85 90Trp Arg Cys Asp Pro Val
Asp Tyr Ser Asn Ser Pro Glu Ala Leu 95 100 105Arg Met Val Arg Val
Ala Trp Leu Phe Leu Phe Ser Lys Phe Ile 110 115 120Glu Leu Met Asp
Thr Val Ile Phe Ile Leu Arg Lys Lys Asp Gly 125 130 135Gln Val Thr
Phe Leu His Val Phe His His Ser Val Leu Pro Trp 140 145 150Ser
Trp Trp Trp Gly Val Lys Ile Ala Pro Gly Gly Met Gly Ser 155 160
165Phe His Ala Met Ile Asn Ser Ser Val His Val Ile Met Tyr Leu 170
175 180Tyr Tyr Gly Leu Ser Ala Phe Gly Pro Val Ala Gln Pro Tyr Leu
185 190 195Trp Trp Lys Lys His Met Thr Ala Ile Gln Leu Ile Gln Phe
Val 200 205 210Leu Val Ser Leu His Ile Ser Gln Tyr Tyr Phe Met Ser
Ser Cys 215 220 225Asn Tyr Gln Tyr Pro Val Ile Ile His Leu Ile Trp
Met Tyr Gly 230 235 240Thr Ile Phe Phe Met Leu Phe Ser Asn Phe Trp
Tyr His Ser Tyr 245 250 255Thr Lys Gly Lys Arg Leu Pro Arg Ala Leu
Gln Gln Asn Gly Ala 260 265 270Pro Gly Ile Ala Lys Val Lys Ala Asn
27540154PRTHomo sapiensmisc_featureIncyte ID No 2198863 40Met Gly
Lys Ser Ala Ser Lys Gln Phe His Asn Glu Val Leu Lys1 5 10 15Ala His
Asn Glu Tyr Arg Gln Lys His Gly Val Pro Pro Leu Lys 20 25 30Leu Cys
Lys Asn Leu Asn Arg Glu Ala Gln Gln Tyr Ser Glu Ala 35 40 45Leu Ala
Ser Thr Arg Ile Leu Lys His Ser Pro Glu Ser Ser Arg 50 55 60Gly Gln
Cys Gly Glu Asn Leu Ala Trp Ala Ser Tyr Asp Gln Thr 65 70 75Gly Lys
Glu Val Ala Asp Arg Trp Tyr Ser Glu Ile Lys Asn Tyr 80 85 90Asn Phe
Gln Gln Pro Gly Phe Thr Ser Gly Thr Gly His Phe Thr 95 100 105Ala
Met Val Trp Lys Asn Thr Lys Lys Met Gly Val Gly Lys Ala 110 115
120Ser Ala Ser Asp Gly Ser Ser Phe Val Val Ala Arg Tyr Phe Pro 125
130 135Ala Gly Asn Val Val Asn Glu Gly Phe Phe Glu Glu Asn Val Leu
140 145 150Pro Pro Lys Lys 41582PRTHomo sapiensmisc_featureIncyte
ID No 3250703 41Met Lys Pro Asn Ile Ile Phe Val Leu Ser Leu Leu Leu
Ile Leu1 5 10 15Glu Lys Gln Ala Ala Val Met Gly Gln Lys Gly Gly Ser
Lys Gly 20 25 30Arg Leu Pro Ser Glu Phe Ser Gln Phe Pro His Gly Gln
Lys Gly 35 40 45Gln His Tyr Ser Gly Gln Lys Gly Lys Gln Gln Thr Glu
Ser Lys 50 55 60Gly Ser Phe Ser Ile Gln Tyr Thr Tyr His Val Asp Ala
Asn Asp 65 70 75His Asp Gln Ser Arg Lys Ser Gln Gln Tyr Asp Leu Asn
Ala Leu 80 85 90His Lys Thr Thr Lys Ser Gln Arg His Leu Gly Gly Ser
Gln Gln 95 100 105Leu Leu His Asn Lys Gln Glu Gly Arg Asp His Asp
Lys Ser Lys 110 115 120Gly His Phe His Arg Val Val Ile His His Lys
Gly Gly Lys Ala 125 130 135His Arg Gly Thr Gln Asn Pro Ser Gln Asp
Gln Gly Asn Ser Pro 140 145 150Ser Gly Lys Gly Ile Ser Ser Gln Tyr
Ser Asn Thr Glu Glu Arg 155 160 165Leu Trp Val His Gly Leu Ser Lys
Glu Gln Thr Ser Val Ser Gly 170 175 180Ala Gln Lys Gly Arg Lys Gln
Gly Gly Ser Gln Ser Ser Tyr Val 185 190 195Leu Gln Thr Glu Glu Leu
Val Ala Asn Lys Gln Gln Arg Glu Thr 200 205 210Lys Asn Ser His Gln
Asn Lys Gly His Tyr Gln Asn Val Val Glu 215 220 225Val Arg Glu Glu
His Ser Ser Lys Val Gln Thr Ser Leu Cys Pro 230 235 240Ala His Gln
Asp Lys Leu Gln His Gly Ser Lys Asp Ile Phe Ser 245 250 255Thr Gln
Asp Glu Leu Leu Val Tyr Asn Lys Asn Gln His Gln Thr 260 265 270Lys
Asn Leu Asn Gln Asp Gln Gln His Gly Arg Lys Ala Asn Lys 275 280
285Ile Ser Tyr Gln Ser Ser Ser Thr Glu Glu Arg Arg Leu His Tyr 290
295 300Gly Glu Asn Gly Val Gln Lys Asp Val Ser Gln Ser Ser Ile Tyr
305 310 315Ser Gln Thr Glu Glu Lys Ile His Gly Lys Ser Gln Asn Gln
Val 320 325 330Thr Ile His Ser Gln Asp Gln Glu His Gly His Lys Glu
Asn Lys 335 340 345Ile Ser Tyr Gln Ser Ser Ser Thr Glu Glu Arg His
Leu Asn Cys 350 355 360Gly Glu Lys Gly Ile Gln Lys Gly Val Ser Lys
Gly Ser Ile Ser 365 370 375Ile Gln Thr Glu Glu Gln Ile His Gly Lys
Ser Gln Asn Gln Val 380 385 390Arg Ile Pro Ser Gln Ala Gln Glu Tyr
Gly His Lys Glu Asn Lys 395 400 405Ile Ser Tyr Gln Ser Ser Ser Thr
Glu Glu Arg Arg Leu Asn Ser 410 415 420Gly Glu Lys Asp Val Gln Lys
Gly Val Ser Lys Gly Ser Ile Ser 425 430 435Ile Gln Thr Glu Glu Lys
Ile His Gly Lys Ser Gln Asn Gln Val 440 445 450Thr Ile Pro Ser Gln
Asp Gln Glu His Gly His Lys Glu Asn Lys 455 460 465Met Ser Tyr Gln
Ser Ser Ser Thr Glu Glu Arg Arg Leu Asn Tyr 470 475 480Gly Gly Lys
Ser Thr Gln Lys Asp Val Ser Gln Ser Ser Ile Ser 485 490 495Phe Gln
Ile Glu Lys Leu Val Glu Gly Lys Ser Gln Ile Gln Thr 500 505 510Pro
Asn Pro Asn Gln Asp Gln Trp Ser Gly Gln Asn Ala Lys Gly 515 520
525Lys Ser Gly Gln Ser Ala Asp Ser Lys Gln Asp Leu Leu Ser His 530
535 540Glu Gln Lys Gly Arg Tyr Lys Gln Glu Ser Ser Glu Ser His Asn
545 550 555Ile Val Ile Thr Glu His Glu Val Ala Gln Asp Asp His Leu
Thr 560 565 570Gln Gln Tyr Asn Glu Asp Arg Asn Pro Ile Ser Thr 575
5804271PRTHomo sapiensmisc_featureIncyte ID No 350287 42Met Phe Thr
Ala Pro Leu Phe Phe Phe Phe Phe Phe Glu Ile Ile1 5 10 15Asn Ser Met
Arg Asn Leu Gly Leu Asn Ile Cys Leu Leu Cys Leu 20 25 30Leu Ile Glu
His His Ser Arg Pro Ser Val Cys Leu Pro Phe Thr 35 40 45Pro Lys Ile
Phe Thr Lys Lys Ile Leu Arg Gln Gln Val Thr Ile 50 55 60Tyr Arg Cys
Leu Asn Asp Phe Leu Ile Phe Ile 65 7043102PRTHomo
sapiensmisc_featureIncyte ID No 1618171 43Met Ala Val Leu Pro Ser
Val Leu Leu Val Tyr Ser Leu Phe Phe1 5 10 15Cys Leu Arg Phe Cys Met
Leu Leu Leu Leu Pro Ser Tyr Ser His 20 25 30Ser Arg Ser Gly Arg Gly
Pro Gly Arg Tyr Gly His Ile Thr Leu 35 40 45Ile Asp Val Ile His Val
Ser Val Tyr Trp Phe Phe Glu Ala Leu 50 55 60Ser Thr Phe Gln Ile Phe
Tyr Tyr Cys Ile Thr Arg Thr Ile Thr 65 70 75Val Arg Lys Gly Ile Val
Val Ser Arg His Val Asn Glu Ala Gly 80 85 90Val Ser Phe Val Ser Tyr
Leu Cys Ile Asn Phe Lys 95 10044226PRTHomo
sapiensmisc_featureIncyte ID No 1625863 44Met Pro Thr Thr Lys Lys
Thr Leu Met Phe Leu Ser Ser Phe Phe1 5 10 15Thr Ser Leu Gly Ser Phe
Ile Val Ile Cys Ser Ile Leu Gly Thr 20 25 30Gln Ala Trp Ile Thr Ser
Thr Ile Ala Val Arg Asp Ser Ala Ser 35 40 45Asn Gly Ser Ile Phe Ile
Thr Tyr Gly Leu Phe Arg Gly Glu Ser 50 55 60Ser Glu Glu Leu Ser His
Gly Leu Ala Glu Pro Lys Lys Lys Phe 65 70 75Ala Val Leu Glu Ile Leu
Asn Asn Ser Ser Gln Lys Thr Leu His 80 85 90Ser Val Thr Ile Leu Phe
Leu Val Leu Ser Leu Ile Thr Ser Leu 95 100 105Leu Ser Ser Gly Phe
Thr Phe Tyr Asn Ser Ile Ser Asn Pro Tyr 110 115 120Gln Thr Phe Leu
Gly Pro Thr Gly Val Tyr Thr Trp Asn Gly Leu 125 130 135Gly Ala Ser
Phe Val Phe Val Thr Met Ile Leu Phe Val Ala Asn 140 145 150Thr Gln
Ser Asn Gln Leu Ser Glu Glu Leu Phe Gln Met Leu Tyr 155 160 165Pro
Ala Thr Thr Ser Lys Gly Thr Thr His Ser Tyr Gly Tyr Ser 170 175
180Phe Trp Leu Ile Leu Leu Val Ile Leu Leu Asn Ile Val Thr Val 185
190 195Thr Ile Ile Ile Phe Tyr Gln Lys Ala Arg Tyr Gln Arg Lys Gln
200 205 210Glu Gln Arg Lys Pro Met Glu Tyr Ala Pro Arg Asp Gly Ile
Leu 215 220 225Phe45154PRTHomo sapiensmisc_featureIncyte ID No
1638353 45Met Ala Leu Leu Leu Ser Val Leu Arg Val Leu Leu Gly Gly
Phe1 5 10 15Phe Ala Leu Val Gly Leu Ala Lys Leu Ser Glu Glu Ile Ser
Ala 20 25 30Pro Val Ser Glu Arg Met Asn Ala Leu Phe Val Gln Phe Ala
Glu 35 40 45Val Phe Pro Leu Lys Val Phe Gly Tyr Gln Pro Asp Pro Leu
Asn 50 55 60Tyr Gln Ile Ala Val Gly Phe Leu Glu Leu Leu Ala Gly Leu
Leu 65 70 75Leu Val Met Gly Pro Pro Met Leu Gln Glu Ile Ser Asn Leu
Phe 80 85 90Leu Ile Leu Leu Met Met Gly Ala Ile Phe Thr Leu Ala Ala
Leu 95 100 105Lys Glu Ser Leu Ser Thr Cys Ile Pro Ala Ile Val Cys
Leu Gly 110 115 120Phe Leu Leu Leu Leu Asn Val Gly Gln Leu Leu Ala
Gln Thr Lys 125 130 135Lys Val Val Arg Pro Thr Arg Lys Lys Thr Leu
Ser Thr Phe Lys 140 145 150Glu Ser Trp Lys 46167PRTHomo
sapiensmisc_featureIncyte ID No 1726843 46Met Ala Ser Pro Arg Thr
Val Thr Ile Val Ala Leu Ser Val Ala1 5 10 15Leu Gly Leu Phe Phe Val
Phe Met Gly Thr Ile Lys Leu Thr Pro 20 25 30Arg Leu Ser Lys Asp Ala
Tyr Ser Glu Met Lys Arg Ala Tyr Lys 35 40 45Ser Tyr Val Arg Ala Leu
Pro Leu Leu Lys Lys Met Gly Ile Asn 50 55 60Ser Ile Leu Leu Arg Lys
Ser Ile Gly Ala Leu Glu Val Ala Cys 65 70 75Gly Ile Val Met Thr Leu
Val Pro Gly Arg Pro Lys Asp Val Ala 80 85 90Asn Phe Phe Leu Leu Leu
Leu Val Leu Ala Val Leu Phe Phe His 95 100 105Gln Leu Val Gly Asp
Pro Leu Lys Arg Tyr Ala His Ala Leu Val 110 115 120Phe Gly Ile Leu
Leu Thr Cys Arg Leu Leu Ile Ala Arg Lys Pro 125 130 135Glu Asp Arg
Ser Ser Glu Lys Lys Pro Leu Pro Gly Asn Ala Glu 140 145 150Glu Gln
Pro Ser Leu Tyr Glu Lys Ala Pro Gln Gly Lys Val Lys 155 160 165Val
Ser47545PRTHomo sapiensmisc_featureIncyte ID No 1754506 47Met Ala
Gly Ala Ile Ile Glu Asn Met Ser Thr Lys Lys Leu Cys1 5 10 15Ile Val
Gly Gly Ile Leu Leu Val Phe Gln Ile Ile Ala Phe Leu 20 25 30Val Gly
Gly Leu Ile Ala Pro Gly Pro Thr Thr Ala Val Ser Tyr 35 40 45Met Ser
Val Lys Cys Val Asp Ala Arg Lys Asn His His Lys Thr 50 55 60Lys Trp
Phe Val Pro Trp Gly Pro Asn His Cys Asp Lys Ile Arg 65 70 75Asp Ile
Glu Glu Ala Ile Pro Arg Glu Ile Glu Ala Asn Asp Ile 80 85 90Val Phe
Ser Val His Ile Pro Leu Pro His Met Glu Met Ser Pro 95 100 105Trp
Phe Gln Phe Met Leu Phe Ile Leu Gln Leu Asp Ile Ala Phe 110 115
120Lys Leu Asn Asn Gln Ile Arg Glu Asn Ala Glu Val Ser Met Asp 125
130 135Val Ser Leu Ala Tyr Arg Asp Asp Ala Phe Ala Glu Trp Thr Glu
140 145 150Met Ala His Glu Arg Val Pro Arg Lys Leu Lys Cys Thr Phe
Thr 155 160 165Ser Pro Lys Thr Pro Glu His Glu Gly Arg Tyr Tyr Glu
Cys Asp 170 175 180Val Leu Pro Phe Met Glu Ile Gly Ser Val Ala His
Lys Phe Tyr 185 190 195Leu Leu Asn Ile Arg Leu Pro Val Asn Glu Lys
Lys Lys Ile Asn 200 205 210Val Gly Ile Gly Glu Ile Lys Asp Ile Arg
Leu Val Gly Ile His 215 220 225Gln Asn Gly Gly Phe Thr Lys Val Trp
Phe Ala Met Lys Thr Phe 230 235 240Leu Thr Pro Ser Ile Phe Ile Ile
Met Val Trp Tyr Trp Arg Arg 245 250 255Ile Thr Met Met Ser Arg Pro
Pro Val Leu Leu Glu Lys Val Ile 260 265 270Phe Ala Leu Gly Ile Ser
Met Thr Phe Ile Asn Ile Pro Val Glu 275 280 285Trp Phe Ser Ile Gly
Phe Asp Trp Thr Trp Met Leu Leu Phe Gly 290 295 300Asp Ile Arg Gln
Gly Ile Phe Tyr Ala Met Leu Leu Ser Phe Trp 305 310 315Ile Ile Phe
Cys Gly Glu His Met Met Asp Gln His Glu Arg Asn 320 325 330His Ile
Ala Gly Tyr Trp Lys Gln Val Gly Pro Ile Ala Val Gly 335 340 345Ser
Phe Cys Leu Phe Ile Phe Asp Met Cys Glu Arg Gly Val Gln 350 355
360Leu Thr Asn Pro Phe Tyr Ser Ile Trp Thr Thr Asp Ile Gly Thr 365
370 375Glu Leu Ala Met Ala Phe Ile Ile Val Ala Gly Ile Cys Leu Cys
380 385 390Leu Tyr Phe Leu Phe Leu Cys Phe Met Val Phe Gln Val Phe
Arg 395 400 405Asn Ile Ser Gly Lys Gln Ser Ser Leu Pro Ala Met Ser
Lys Val 410 415 420Arg Arg Leu His Tyr Glu Gly Leu Ile Phe Arg Phe
Lys Phe Leu 425 430 435Met Leu Ile Thr Leu Ala Cys Ala Ala Met Thr
Val Ile Phe Phe 440 445 450Ile Val Ser Gln Val Thr Glu Gly His Trp
Lys Trp Gly Gly Val 455 460 465Thr Val Gln Val Asn Ser Ala Phe Phe
Thr Gly Ile Tyr Gly Met 470 475 480Trp Asn Leu Tyr Val Phe Ala Leu
Met Phe Leu Tyr Ala Pro Ser 485 490 495His Lys Asn Tyr Gly Glu Asp
Gln Ser Asn Gly Met Gln Leu Pro 500 505 510Cys Lys Ser Arg Glu Asp
Cys Ala Leu Phe Val Ser Glu Leu Tyr 515 520 525Gln Glu Leu Phe Ser
Ala Ser Lys Tyr Ser Phe Ile Asn Asp Asn 530 535 540Ala Ala Ser Gly
Ile 54548570PRTHomo sapiensmisc_featureIncyte ID No 1831378 48Met
Gly Phe Leu Gln Leu Leu Val Val Ala Val Leu Ala Ser Glu1 5 10 15His
Arg Val Ala Gly Ala Ala Glu Val Phe Gly Asn Ser Ser Glu 20 25 30Gly
Leu Ile Glu Phe Ser Val Gly Lys Phe Arg Tyr Phe Glu Leu 35 40 45Asn
Arg Pro Phe Pro Glu Glu Ala Ile Leu His Asp Ile Ser Ser 50 55 60Asn
Val Thr Phe Leu Ile Phe Gln Ile His Ser Gln Tyr Gln Asn 65 70 75Thr
Thr Val Ser Phe Ser Pro Thr Leu Leu Ser Asn Ser Ser Glu 80 85 90Thr
Gly Thr Ala Ser Gly Leu Val Phe Ile Leu Arg Pro Glu Gln 95 100
105Ser Thr Cys Thr Trp Tyr Leu Gly Thr Ser Gly Ile Gln Pro Val 110
115 120Gln Asn Met Ala Ile Leu Leu Ser Tyr Ser Glu Arg Asp Pro Val
125 130 135Pro Gly Gly Cys Asn Leu Glu Phe Asp Leu Asp Ile Asp Pro
Asn 140 145
150Ile Tyr Leu Glu Tyr Asn Phe Phe Glu Thr Thr Ile Lys Phe Ala 155
160 165Pro Ala Asn Leu Gly Tyr Ala Arg Gly Val Asp Pro Pro Pro Cys
170 175 180Asp Ala Gly Thr Asp Gln Asp Ser Arg Trp Arg Leu Gln Tyr
Asp 185 190 195Val Tyr Gln Tyr Phe Leu Pro Glu Asn Asp Leu Thr Glu
Glu Met 200 205 210Leu Leu Lys His Leu Gln Arg Met Val Ser Val Pro
Gln Val Lys 215 220 225Ala Ser Ala Leu Lys Val Val Thr Leu Thr Ala
Asn Asp Lys Thr 230 235 240Ser Val Ser Phe Ser Ser Leu Pro Gly Gln
Gly Val Ile Tyr Asn 245 250 255Val Ile Val Trp Asp Pro Phe Leu Asn
Thr Ser Ala Ala Tyr Ile 260 265 270Pro Ala His Thr Tyr Ala Cys Ser
Phe Glu Ala Gly Glu Gly Ser 275 280 285Cys Ala Ser Leu Gly Arg Val
Ser Ser Lys Val Phe Phe Thr Leu 290 295 300Phe Ala Leu Leu Gly Phe
Phe Ile Cys Phe Phe Gly His Arg Phe 305 310 315Trp Lys Thr Glu Leu
Phe Phe Ile Gly Phe Ile Ile Met Gly Phe 320 325 330Phe Phe Tyr Ile
Leu Ile Thr Arg Leu Thr Pro Ile Lys Tyr Asp 335 340 345Val Asn Leu
Ile Leu Thr Ala Val Thr Gly Ser Val Gly Gly Met 350 355 360Phe Leu
Val Ala Val Trp Trp Arg Phe Gly Ile Leu Ser Ile Cys 365 370 375Met
Leu Cys Val Gly Leu Val Leu Gly Phe Leu Ile Ser Ser Val 380 385
390Thr Phe Phe Thr Pro Leu Gly Asn Leu Lys Ile Phe His Asp Asp 395
400 405Gly Val Phe Trp Val Thr Phe Ser Cys Ile Ala Ile Leu Ile Pro
410 415 420Val Val Phe Met Gly Cys Leu Arg Ile Leu Asn Ile Leu Thr
Cys 425 430 435Gly Val Ile Gly Ser Tyr Ser Val Val Leu Ala Ile Asp
Ser Tyr 440 445 450Trp Ser Thr Ser Leu Ser Tyr Ile Thr Leu Asn Val
Leu Lys Arg 455 460 465Ala Leu Asn Lys Asp Phe His Arg Ala Phe Thr
Asn Val Pro Phe 470 475 480Gln Thr Asn Asp Phe Ile Ile Leu Ala Val
Trp Gly Met Leu Ala 485 490 495Val Ser Gly Ile Thr Leu Gln Ile Arg
Arg Glu Arg Gly Arg Pro 500 505 510Phe Phe Pro Pro His Pro Tyr Lys
Leu Trp Lys Gln Glu Arg Glu 515 520 525Arg Arg Val Thr Asn Ile Leu
Asp Pro Ser Tyr His Ile Pro Pro 530 535 540Leu Arg Glu Arg Leu Tyr
Gly Arg Leu Thr Gln Ile Lys Gly Leu 545 550 555Phe Gln Lys Glu Gln
Pro Ala Gly Glu Arg Thr Pro Leu Leu Leu 560 565 57049127PRTHomo
sapiensmisc_featureIncyte ID No 1864943 49Met Arg Arg Arg Phe Trp
Gly Val Phe Asn Cys Leu Cys Ala Gly1 5 10 15Ala Phe Gly Ala Leu Ala
Ala Ala Ser Ala Lys Leu Ala Phe Gly 20 25 30Ser Glu Val Ser Met Gly
Leu Cys Val Leu Gly Ile Ile Val Met 35 40 45Ala Ser Thr Asn Ser Leu
Met Trp Thr Phe Phe Ser Arg Gly Leu 50 55 60Ser Phe Ser Met Ser Ser
Ala Ile Ala Ser Val Thr Val Thr Phe 65 70 75Ser Asn Ile Leu Ser Ser
Ala Phe Leu Gly Tyr Val Leu Tyr Gly 80 85 90Glu Cys Gln Glu Val Leu
Trp Trp Gly Gly Val Phe Leu Ile Leu 95 100 105Cys Gly Leu Thr Leu
Ile His Arg Lys Leu Pro Pro Thr Trp Lys 110 115 120Pro Leu Pro His
Lys Gln Gln 12550152PRTHomo sapiensmisc_featureIncyte ID No 1911316
50Met Asp Asn Val Gln Pro Lys Ile Lys His Arg Pro Phe Cys Phe1 5 10
15Ser Val Lys Gly His Val Lys Met Leu Arg Leu Ala Leu Thr Val 20 25
30Thr Ser Met Thr Phe Phe Ile Ile Ala Gln Ala Pro Glu Pro Tyr 35 40
45Ile Val Ile Thr Gly Phe Glu Val Thr Val Ile Leu Phe Phe Ile 50 55
60Leu Leu Tyr Val Leu Arg Leu Asp Arg Leu Met Lys Trp Leu Phe 65 70
75Trp Pro Leu Leu Asp Ile Ile Asn Ser Leu Val Thr Thr Val Phe 80 85
90Met Leu Ile Val Ser Val Leu Ala Leu Ile Pro Glu Thr Thr Thr 95
100 105Leu Thr Val Gly Gly Gly Val Phe Ala Leu Val Thr Ala Val Cys
110 115 120Cys Leu Ala Asp Gly Ala Leu Ile Tyr Arg Lys Leu Leu Phe
Asn 125 130 135Pro Ser Gly Pro Tyr Gln Lys Lys Pro Val His Glu Lys
Lys Glu 140 145 150Val Leu 51777PRTHomo sapiensmisc_featureIncyte
ID No 1943120 51Met Thr Phe Tyr Pro Phe Val Ala Ser Ser Ser Thr Arg
Arg Val1 5 10 15Asp Asn Ser Asn Thr Arg Leu Ala Val Gln Ile Glu Arg
Asp Pro 20 25 30Gly Asn Asp Asp Asn Asn Leu Asn Ser Ile Phe Tyr Glu
His Leu 35 40 45Thr Arg Thr Leu Leu Glu Ser Leu Cys Gly Asp Leu Val
Leu Gly 50 55 60Arg Trp Gly Asn Tyr Ser Ser Gly Asp Cys Phe Ile Leu
Ala Ser 65 70 75Asp Asp Leu Asn Ala Phe Val His Leu Ile Glu Ile Gly
Asn Gly 80 85 90Leu Val Thr Phe Gln Leu Arg Gly Leu Glu Phe Arg Gly
Thr Tyr 95 100 105Cys Gln Gln Arg Glu Val Glu Ala Ile Met Glu Gly
Asp Glu Glu 110 115 120Asp Arg Gly Cys Cys Cys Cys Lys Pro Gly His
Leu Pro His Leu 125 130 135Leu Ser Arg Asn Ala Ala Phe His Leu Arg
Trp Leu Thr Trp Glu 140 145 150Ile Thr Gln Thr Gln Tyr Ile Leu Glu
Gly Tyr Ser Ile Leu Asp 155 160 165Asn Asn Ala Ala Thr Met Leu Gln
Val Phe Asp Leu Arg Arg Ile 170 175 180Leu Ile Arg Tyr Tyr Ile Lys
Ser Ile Ile Tyr Tyr Met Val Thr 185 190 195Ser Pro Lys Leu Leu Ser
Trp Ile Lys Asn Glu Ser Leu Leu Lys 200 205 210Ser Leu Gln Pro Phe
Ala Lys Trp His Tyr Ile Glu Arg Asp Leu 215 220 225Ala Met Phe Asn
Ile Asn Ile Asp Asp Asp Tyr Val Pro Cys Leu 230 235 240Gln Gly Ile
Thr Arg Ala Ser Phe Cys Asn Val Tyr Leu Glu Trp 245 250 255Ile Gln
His Cys Ala Arg Lys Arg Gln Glu Pro Ser Thr Thr Leu 260 265 270Asp
Ser Asp Glu Asp Ser Pro Leu Val Thr Leu Ser Phe Ala Leu 275 280
285Cys Thr Leu Gly Arg Arg Ala Leu Gly Thr Ala Ala His Asn Met 290
295 300Ala Ile Ser Leu Asp Ser Phe Leu Tyr Gly Leu His Val Leu Phe
305 310 315Lys Gly Asp Phe Arg Ile Thr Ala Arg Asp Glu Trp Val Phe
Ala 320 325 330Asp Met Asp Leu Leu His Lys Val Val Ala Pro Ala Ile
Arg Met 335 340 345Ser Leu Lys Leu His Gln Asp Gln Phe Thr Cys Pro
Asp Glu Tyr 350 355 360Glu Asp Pro Ala Val Leu Tyr Glu Ala Ile Gln
Ser Phe Glu Lys 365 370 375Lys Val Val Ile Cys His Glu Gly Asp Pro
Ala Trp Arg Gly Ala 380 385 390Val Leu Ser Asn Lys Glu Glu Leu Leu
Thr Leu Arg His Val Val 395 400 405Asp Glu Gly Ala Asp Glu Tyr Lys
Val Ile Met Leu His Arg Ser 410 415 420Phe Leu Ser Phe Lys Val Ile
Lys Val Asn Lys Glu Cys Val Arg 425 430 435Gly Leu Trp Ala Gly Gln
Gln Gln Glu Leu Ile Phe Leu Arg Asn 440 445 450Arg Asn Pro Glu Arg
Gly Ser Ile Gln Asn Asn Lys Gln Val Leu 455 460 465Arg Asn Leu Ile
Asn Ser Ser Cys Asp Gln Pro Leu Gly Tyr Pro 470 475 480Met Tyr Val
Ser Pro Leu Thr Thr Ser Tyr Leu Gly Thr His Arg 485 490 495Gln Leu
Lys Asn Ile Trp Gly Gly Pro Ile Thr Leu Asp Arg Ile 500 505 510Arg
Thr Trp Phe Trp Thr Lys Trp Val Arg Met Arg Lys Asp Cys 515 520
525Asn Ala Arg Gln His Ser Gly Gly Asn Ile Glu Asp Val Asp Gly 530
535 540Gly Gly Ala Pro Thr Thr Gly Gly Asn Asn Ala Pro Asn Gly Gly
545 550 555Ser Gln Glu Ser Ser Ala Glu Gln Pro Arg Lys Gly Gly Ala
Gln 560 565 570His Gly Val Ser Ser Cys Glu Gly Thr Gln Arg Thr Gly
Arg Arg 575 580 585Lys Gly Arg Ser Gln Ser Val Gln Ala His Ser Ala
Leu Ser Gln 590 595 600Arg Pro Pro Met Leu Ser Ser Ser Gly Pro Ile
Leu Glu Ser Arg 605 610 615Gln Thr Phe Leu Gln Thr Ser Thr Ser Val
His Glu Leu Ala Gln 620 625 630Arg Leu Ser Gly Ser Arg Leu Ser Leu
His Ala Ser Ala Thr Ser 635 640 645Leu His Ser Gln Pro Pro Pro Val
Thr Thr Thr Gly His Leu Ser 650 655 660Val Arg Glu Arg Ala Glu Ala
Leu Ile Arg Ser Ser Leu Gly Ser 665 670 675Ser Thr Ser Ser Thr Leu
Ser Phe Leu Phe Gly Lys Arg Ser Phe 680 685 690Ser Ser Ala Leu Val
Ile Ser Gly Leu Ser Ala Ala Glu Gly Gly 695 700 705Asn Thr Ser Asp
Thr Gln Ser Ser Ser Ser Val Asn Ile Val Met 710 715 720Gly Pro Ser
Ala Arg Ala Ala Ser Gln Ala Thr Arg Val Arg Gly 725 730 735Trp Ala
Gly Leu Thr Arg Thr Gly Trp Asp Gly Gly Thr Gly Ser 740 745 750Trp
Pro Glu Arg Gly Thr Cys Leu Ala Phe Pro Pro Phe Cys Leu 755 760
765Gln Asn Pro Ile Pro Phe Ser Met Gly Leu Pro Glu 770
77552108PRTHomo sapiensmisc_featureIncyte ID No 2314236 52Met Phe
Lys His Glu Leu Glu Glu Leu Arg Thr Thr Ile Met Tyr1 5 10 15Arg Asp
Ser His Ser Val Leu Ala Leu Asn Trp Lys Val Val Ala 20 25 30Thr Leu
Lys Tyr Phe Leu Leu Tyr Val Ile Ile Leu Tyr Asn Leu 35 40 45Glu Arg
Asp Asn Gly His Ser Asn Tyr Glu Asn Tyr Glu Leu Gly 50 55 60Asp Lys
Ser Leu Asn Leu Leu Leu Phe Tyr Asn Ser Met Tyr Lys 65 70 75Leu Val
Phe Pro Tyr Ile Phe Thr Phe Ser Ser Phe Leu Ile Ser 80 85 90Ser Tyr
Thr Ser Ile Leu Tyr Lys Met Phe Tyr Ile Gln Arg Thr 95 100 105Val
Lys Ser5366PRTHomo sapiensmisc_featureIncyte ID No 2479409 53Met
Asn Leu Ser Lys Lys Ser Ile Leu Leu Thr Gln Val Ile Lys1 5 10 15Phe
Val Asp Ile Arg Leu Phe Ile Met Val Pro Ser Tyr Pro Phe 20 25 30Asn
Val Phe Arg Ser Cys Val Asp Asn Phe Leu Phe Ile Met Ile 35 40 45Leu
Val Ile Ser Val Leu Thr Phe Leu Ile Arg Leu Gly Arg Gly 50 55 60Leu
Ser Val Leu Leu Ile 6554540PRTHomo sapiensmisc_featureIncyte ID No
2683149 54Met Met Gly Ser Pro Val Ser His Leu Leu Ala Gly Phe Cys
Val1 5 10 15Trp Val Val Leu Gly Trp Val Gly Gly Ser Val Pro Asn Leu
Gly 20 25 30Pro Ala Glu Gln Glu Gln Asn His Tyr Leu Ala Gln Leu Phe
Gly 35 40 45Leu Tyr Gly Glu Asn Gly Thr Leu Thr Ala Gly Gly Leu Ala
Arg 50 55 60Leu Leu His Ser Leu Gly Leu Gly Arg Val Gln Gly Leu Arg
Leu 65 70 75Gly Gln His Gly Pro Leu Thr Gly Arg Ala Ala Ser Pro Ala
Ala 80 85 90Asp Asn Ser Thr His Arg Pro Gln Asn Pro Glu Leu Ser Val
Asp 95 100 105Val Trp Ala Gly Met Pro Leu Gly Pro Ser Gly Trp Gly
Asp Leu 110 115 120Glu Glu Ser Lys Ala Pro His Leu Pro Arg Gly Pro
Ala Pro Ser 125 130 135Gly Leu Asp Leu Leu His Arg Leu Leu Leu Leu
Asp His Ser Leu 140 145 150Ala Asp His Leu Asn Glu Asp Cys Leu Asn
Gly Ser Gln Leu Leu 155 160 165Val Asn Phe Gly Leu Ser Pro Ala Ala
Pro Leu Thr Pro Arg Gln 170 175 180Phe Ala Leu Leu Cys Pro Ala Leu
Leu Tyr Gln Ile Asp Ser Arg 185 190 195Val Cys Ile Gly Ala Pro Ala
Pro Ala Pro Pro Gly Asp Leu Leu 200 205 210Ser Ala Leu Leu Gln Ser
Ala Leu Ala Val Leu Leu Leu Ser Leu 215 220 225Pro Ser Pro Leu Ser
Leu Leu Leu Leu Arg Leu Leu Gly Pro Arg 230 235 240Leu Leu Arg Pro
Leu Leu Gly Phe Leu Gly Ala Leu Ala Val Gly 245 250 255Thr Leu Cys
Gly Asp Ala Leu Leu His Leu Leu Pro His Ala Gln 260 265 270Glu Gly
Arg His Ala Gly Pro Gly Gly Leu Pro Glu Lys Asp Leu 275 280 285Gly
Pro Gly Leu Ser Val Leu Gly Gly Leu Phe Leu Leu Phe Val 290 295
300Leu Glu Asn Met Leu Gly Leu Leu Arg His Arg Gly Leu Arg Pro 305
310 315Arg Cys Cys Arg Arg Lys Arg Arg Asn Leu Glu Thr Arg Asn Leu
320 325 330Asp Pro Glu Asn Gly Ser Gly Met Ala Leu Gln Pro Leu Gln
Ala 335 340 345Ala Pro Glu Pro Gly Ala Gln Gly Gln Arg Glu Lys Asn
Ser Gln 350 355 360His Pro Pro Ala Leu Ala Pro Pro Gly His Gln Gly
His Ser His 365 370 375Gly His Gln Gly Gly Thr Asp Ile Thr Trp Met
Val Leu Leu Gly 380 385 390Asp Gly Leu His Asn Leu Thr Asp Gly Leu
Ala Ile Gly Ala Ala 395 400 405Phe Ser Asp Gly Phe Ser Ser Gly Leu
Ser Thr Thr Leu Ala Val 410 415 420Phe Cys His Glu Leu Pro His Glu
Leu Gly Asp Phe Ala Met Leu 425 430 435Leu Gln Ser Gly Leu Ser Phe
Arg Arg Leu Leu Leu Leu Ser Leu 440 445 450Val Ser Gly Ala Leu Gly
Leu Gly Gly Ala Val Leu Gly Val Gly 455 460 465Leu Ser Leu Gly Pro
Val Pro Leu Thr Pro Trp Val Phe Gly Val 470 475 480Thr Ala Gly Val
Phe Leu Tyr Val Ala Leu Val Asp Met Leu Pro 485 490 495Ala Leu Leu
Arg Pro Pro Glu Pro Leu Pro Thr Pro His Val Leu 500 505 510Leu Gln
Gly Leu Gly Leu Leu Leu Gly Gly Gly Leu Met Leu Ala 515 520 525Ile
Thr Leu Leu Glu Glu Arg Leu Leu Pro Val Thr Thr Glu Gly 530 535
5405587PRTHomo sapiensmisc_featureIncyte ID No 2774051 55Met Pro
Phe Thr Leu Asp Asp Tyr Gly Ala Tyr Ser Ser Gln Lys1 5 10 15Gln Tyr
Thr Cys Gln Phe Pro Ser Thr Ile Ala Ile His Ala Glu 20 25 30Asp Lys
Arg Pro Pro Gln Ser Arg Arg Gly Ile Val Leu Gly Pro 35 40 45Ile Phe
Leu Ile Val Leu Lys Ile Ile Ile Arg Trp Thr Val Phe 50 55 60Cys Glu
Asp Phe Leu Phe Pro Ser Ser Lys Lys Pro Cys Gly Lys 65 70 75Asn Ser
Leu Ile Thr Val Leu Ile Phe Phe Phe Phe 80 8556100PRTHomo
sapiensmisc_featureIncyte ID No 2869038 56Met Ile Met Ala Gln Lys
Ile Gly Gly Leu Thr Trp Trp Ala Ile1 5 10 15Met Phe Ile Ile Leu Phe
Glu Ile Thr Gly Thr Ser Ser Ser Phe 20 25 30Leu Arg Ile Asn Ala Leu
Pro His Phe Ser Met Asn Arg Cys Gly 35 40 45Glu Ala Tyr Phe Pro Phe
Ser Tyr Leu Tyr Thr Ser Leu Gln Lys 50 55 60Gln Phe Leu Met Lys Val
Ser Gly Ile Val Lys Asn Leu Arg Gly 65 70 75Met Met Thr Gly Gly Val
Trp Gly Phe Phe Leu Tyr Ser Phe Phe 80 85 90Asn Glu Lys Ser Phe Lys
Cys Ser Thr Gly 95 1005758PRTHomo sapiensmisc_featureIncyte ID No
2918334 57Met Asp Leu Leu Tyr Glu Ile Leu Leu Ala Leu Tyr Tyr Asn
Ile1 5 10 15Cys Tyr Asp Ile Pro Phe Ile Phe Phe Asn Leu Asn Met Met
Phe 20 25 30Tyr Ile Val Leu Asp Leu Arg Ile Val Phe Phe Arg Thr Ile
Arg 35 40 45Glu Tyr Leu Ser Pro Pro Ser Leu Ser Phe Tyr Ile Tyr 50
555861PRTHomo sapiensmisc_featureIncyte ID No 2949916 58Met Arg Arg
Ile Ile Arg Leu Arg Leu Arg Phe Ser Asp Thr Phe1 5 10 15Met Ala Ala
Phe Leu Leu Cys Leu Gly Phe Val Leu Met Leu Phe 20 25 30Pro Ser Leu
Leu Arg Asp Gly Gly Ser Ile Ser Ser Cys Arg Asn 35 40 45Ser Cys Ser
Ser Pro Ser Ser Glu Glu Arg His Phe Ser Asn Leu 50 55 60Glu
5950PRTHomo sapiensmisc_featureIncyte ID No 2989375 59Met Cys Leu
Thr Pro His Arg Asp Ser Met Cys Glu Asp Ser Pro1 5 10 15Phe Thr His
Gln Ile Ile Ser Met Ala Thr Ala Cys Ser Leu Leu 20 25 30Leu Glu Cys
Phe Val Leu Ala Ala Ser Leu Leu Val Cys Val Trp 35 40 45Ser Glu Trp
Arg Arg 5060310PRTHomo sapiensmisc_featureIncyte ID No 3316764
60Met Arg Arg Thr Ala Phe Ile Leu Gly Ser Gly Leu Leu Ser Phe1 5 10
15Val Ala Phe Trp Asn Ser Val Thr Trp His Leu Gln Arg Phe Trp 20 25
30Gly Ala Ser Gly Tyr Phe Trp Gln Ala Gln Trp Glu Arg Leu Leu 35 40
45Thr Thr Phe Glu Gly Lys Glu Trp Ile Leu Phe Phe Ile Gly Ala 50 55
60Ile Gln Val Pro Cys Leu Phe Phe Trp Ser Phe Asn Gly Leu Leu 65 70
75Leu Val Val Asp Thr Thr Gly Lys Pro Asn Phe Ile Ser Arg Tyr 80 85
90Arg Ile Gln Val Gly Lys Asn Glu Pro Val Asp Pro Val Lys Leu 95
100 105Arg Gln Ser Ile Arg Thr Val Leu Phe Asn Gln Cys Met Ile Ser
110 115 120Phe Pro Met Val Val Phe Leu Tyr Pro Phe Leu Lys Trp Trp
Arg 125 130 135Asp Pro Cys Arg Arg Glu Leu Pro Thr Phe His Trp Phe
Leu Leu 140 145 150Glu Leu Ala Ile Phe Thr Leu Ile Glu Glu Val Leu
Phe Tyr Tyr 155 160 165Ser His Arg Leu Leu His His Pro Thr Phe Tyr
Lys Lys Ile His 170 175 180Lys Lys His His Glu Trp Thr Ala Pro Ile
Gly Val Ile Ser Leu 185 190 195Tyr Ala His Pro Ile Glu His Ala Val
Ser Asn Met Leu Pro Val 200 205 210Ile Val Gly Pro Leu Val Met Gly
Ser His Leu Ser Ser Ile Thr 215 220 225Met Trp Phe Ser Leu Ala Leu
Ile Ile Thr Thr Ile Ser His Cys 230 235 240Gly Tyr His Leu Pro Phe
Leu Pro Ser Pro Glu Phe His Asp Tyr 245 250 255His His Leu Lys Phe
Asn Gln Cys Tyr Gly Val Leu Gly Val Leu 260 265 270Asp His Leu His
Gly Thr Asp Thr Met Phe Lys Gln Thr Lys Ala 275 280 285Tyr Glu Arg
His Val Leu Leu Leu Gly Phe Thr Pro Leu Ser Glu 290 295 300Ser Ile
Pro Asp Ser Pro Lys Arg Met Glu 305 31061160PRTHomo
sapiensmisc_featureIncyte ID No 3359559 61Met Ala Pro Ala Leu Trp
Arg Ala Cys Asn Gly Leu Met Ala Ala1 5 10 15Phe Phe Ala Leu Ala Ala
Leu Val Gln Val Asn Asp Pro Asp Ala 20 25 30Glu Val Trp Val Val Val
Tyr Thr Ile Pro Ala Val Leu Thr Leu 35 40 45Leu Val Gly Leu Asn Pro
Glu Val Thr Gly Asn Val Ile Trp Lys 50 55 60Ser Ile Ser Ala Ile His
Ile Leu Phe Cys Thr Val Trp Ala Val 65 70 75Gly Leu Ala Ser Tyr Leu
Leu His Arg Thr Gln Gln Asn Ile Leu 80 85 90His Glu Glu Glu Gly Arg
Glu Leu Ser Gly Leu Val Ile Ile Thr 95 100 105Ala Trp Ile Ile Leu
Cys His Ser Ser Ser Lys Asn Pro Val Gly 110 115 120Gly Arg Ile Gln
Leu Ala Ile Ala Ile Val Ile Thr Leu Phe Pro 125 130 135Phe Ile Ser
Trp Val Tyr Ile Tyr Ile Asn Lys Glu Met Arg Ser 140 145 150Ser Trp
Pro Thr His Cys Lys Thr Val Ile 155 1606235PRTHomo
sapiensmisc_featureIncyte ID No 4289208 62Met Ala Val Val Asp Ala
Gly Asn Asn Gly Lys Val Leu Asp Arg1 5 10 15Val Cys Val Arg Ser Val
Pro Ala Leu Phe Leu Ser Lys Cys Ile 20 25 30Ser Leu Asp Met Glu
3563323PRTHomo sapiensmisc_featureIncyte ID No 2454013 63Met Ala
Ala Pro Lys Gly Ser Leu Trp Val Arg Thr Gln Leu Gly1 5 10 15Leu Pro
Pro Leu Leu Leu Leu Thr Met Ala Leu Ala Gly Gly Ser 20 25 30Gly Thr
Ala Ser Ala Glu Ala Phe Asp Ser Val Leu Gly Asp Thr 35 40 45Ala Ser
Cys His Arg Ala Cys Gln Leu Thr Tyr Pro Leu His Thr 50 55 60Tyr Pro
Lys Glu Glu Glu Leu Tyr Ala Cys Gln Arg Gly Cys Arg 65 70 75Leu Phe
Ser Ile Cys Gln Phe Val Asp Asp Gly Ile Asp Leu Asn 80 85 90Arg Thr
Lys Leu Glu Cys Glu Ser Ala Cys Thr Glu Ala Tyr Ser 95 100 105Gln
Ser Asp Glu Gln Tyr Ala Cys His Leu Gly Cys Gln Asn Gln 110 115
120Leu Pro Phe Ala Glu Leu Arg Gln Glu Gln Leu Met Ser Leu Met 125
130 135Pro Lys Met His Leu Leu Phe Pro Leu Thr Leu Val Arg Ser Phe
140 145 150Trp Ser Asp Met Met Asp Ser Ala Gln Ser Phe Ile Thr Ser
Ser 155 160 165Trp Thr Phe Tyr Leu Gln Ala Asp Asp Gly Lys Ile Val
Ile Phe 170 175 180Gln Ser Lys Pro Glu Ile Gln Tyr Ala Pro His Leu
Glu Gln Glu 185 190 195Pro Thr Asn Leu Arg Glu Ser Ser Leu Ser Lys
Met Ser Tyr Leu 200 205 210Gln Met Arg Asn Ser Gln Ala His Arg Asn
Phe Leu Glu Asp Gly 215 220 225Glu Ser Asp Gly Phe Leu Arg Cys Leu
Ser Leu Asn Ser Gly Trp 230 235 240Ile Leu Thr Thr Thr Leu Val Leu
Ser Val Met Val Leu Leu Trp 245 250 255Ile Cys Cys Ala Thr Val Ala
Thr Ala Val Glu Gln Tyr Val Pro 260 265 270Ser Glu Lys Leu Ser Ile
Tyr Gly Asp Leu Glu Phe Met Asn Glu 275 280 285Gln Lys Leu Asn Arg
Tyr Pro Ala Ser Ser Leu Val Val Val Arg 290 295 300Ser Lys Thr Glu
Asp His Glu Glu Ala Gly Pro Leu Pro Thr Lys 305 310 315Val Asn Leu
Ala His Ser Glu Ile 32064129PRTHomo sapiensmisc_featureIncyte ID No
2454048 64Met Ala Arg Gly Ser Leu Arg Arg Leu Leu Arg Leu Leu Val
Leu1 5 10 15Gly Leu Trp Leu Ala Leu Leu Arg Ser Val Ala Gly Glu Gln
Ala 20 25 30Pro Gly Thr Ala Pro Cys Ser Arg Gly Ser Ser Trp Ser Ala
Asp 35 40 45Leu Asp Lys Cys Met Asp Cys Ala Ser Cys Arg Ala Arg Pro
His 50 55 60Ser Asp Phe Cys Leu Gly Cys Ala Ala Ala Pro Pro Ala Pro
Phe 65 70 75Arg Leu Leu Trp Pro Ile Leu Gly Gly Ala Leu Ser Leu Thr
Phe 80 85 90Val Leu Gly Leu Leu Ser Gly Phe Leu Val Trp Arg Arg Cys
Arg 95 100 105Arg Arg Glu Lys Phe Thr Thr Pro Ile Glu Glu Thr Gly
Gly Glu 110 115 120Gly Cys Pro Ala Val Ala Leu Ile Gln
12565461PRTHomo sapiensmisc_featureIncyte ID No 2479282 65Met Ala
Pro Gln Ser Leu Pro Ser Ser Arg Met Ala Pro Leu Gly1 5 10 15Met Leu
Leu Gly Leu Leu Met Ala Ala Cys Phe Thr Phe Cys Leu 20 25 30Ser His
Gln Asn Leu Lys Glu Phe Ala Leu Thr Asn Pro Glu Lys 35 40 45Ser Ser
Thr Lys Glu Thr Glu Arg Lys Glu Thr Lys Ala Glu Glu 50 55 60Glu Leu
Asp Ala Glu Val Leu Glu Val Phe His Pro Thr His Glu 65 70 75Trp Gln
Ala Leu Gln Pro Gly Gln Ala Val Pro Ala Gly Ser His 80 85 90Val Arg
Leu Asn Leu Gln Thr Gly Glu Arg Glu Ala Lys Leu Gln 95 100 105Tyr
Glu Asp Lys Phe Arg Asn Asn Leu Lys Gly Lys Arg Leu Asp 110 115
120Ile Asn Thr Asn Thr Tyr Thr Ser Gln Asp Leu Lys Ser Ala Leu 125
130 135Ala Lys Phe Lys Glu Gly Ala Glu Met Glu Ser Ser Lys Glu Asp
140 145 150Lys Ala Arg Gln Ala Glu Val Lys Arg Leu Phe Arg Pro Ile
Glu 155 160 165Glu Leu Lys Lys Asp Phe Asp Glu Leu Asn Val Val Ile
Glu Thr 170 175 180Asp Met Gln Ile Met Val Arg Leu Ile Asn Lys Phe
Asn Ser Ser 185 190 195Ser Ser Ser Leu Glu Glu Lys Ile Ala Ala Leu
Phe Asp Leu Glu 200 205 210Tyr Tyr Val His Gln Met Asp Asn Ala Gln
Asp Leu Leu Ser Phe 215 220 225Gly Gly Leu Gln Val Val Ile Asn Gly
Leu Asn Ser Thr Glu Pro 230 235 240Leu Val Lys Glu Tyr Ala Ala Phe
Val Leu Gly Ala Ala Phe Ser 245 250 255Ser Asn Pro Lys Val Gln Val
Glu Ala Ile Glu Gly Gly Ala Leu 260 265 270Gln Lys Leu Leu Val Ile
Leu Ala Thr Glu Gln Pro Leu Thr Ala 275 280 285Lys Lys Lys Val Leu
Phe Ala Leu Cys Ser Leu Leu Arg His Phe 290 295 300Pro Tyr Ala Gln
Arg Gln Phe Leu Lys Leu Gly Gly Leu Gln Val 305 310 315Leu Arg Thr
Leu Val Gln Glu Lys Gly Thr Glu Val Leu Ala Val 320 325 330Arg Val
Val Thr Leu Leu Tyr Asp Leu Val Thr Glu Lys Met Phe 335 340 345Ala
Glu Glu Glu Ala Glu Leu Thr Gln Glu Met Ser Pro Glu Lys 350 355
360Leu Gln Gln Tyr Arg Gln Val His Leu Leu Pro Gly Leu Trp Glu 365
370 375Gln Gly Trp Cys Glu Ile Thr Ala His Leu Leu Ala Leu Pro Glu
380 385 390His Asp Ala Arg Glu Lys Val Leu Gln Thr Leu Gly Val Leu
Leu 395 400 405Thr Thr Cys Arg Asp Arg Tyr Arg Gln Asp Pro Gln Leu
Gly Arg 410 415 420Thr Leu Ala Ser Leu Gln Ala Glu Tyr Gln Val Leu
Ala Ser Leu 425 430 435Glu Leu Gln Asp Gly Glu Asp Glu Gly Tyr Phe
Gln Glu Leu Leu 440 445 450Gly Ser Val Asn Ser Leu Leu Lys Glu Leu
Arg 455 46066264PRTHomo sapiensmisc_featureIncyte ID No 2483432
66Met Arg Pro Leu Leu Gly Leu Leu Leu Val Phe Ala Gly Cys Thr1 5 10
15Phe Ala Leu Tyr Leu Leu Ser Thr Arg Leu Pro Arg Gly Arg Arg 20 25
30Leu Gly Ser Thr Glu Glu Ala Gly Gly Arg Ser Leu Trp Phe Pro 35 40
45Ser Asp Leu Ala Glu Leu Arg Glu Leu Ser Glu Val Leu Arg Glu 50 55
60Tyr Arg Lys Glu His Gln Ala Tyr Val Phe Leu Leu Phe Cys Gly 65 70
75Ala Tyr Leu Tyr Lys Gln Gly Phe Ala Ile Pro Gly Ser Ser Phe 80 85
90Leu Asn Val Leu Ala Gly Ala Leu Phe Gly Pro Trp Leu Gly Leu 95
100 105Leu Leu Cys Cys Val Leu Thr Ser Val Gly Ala Thr Cys Cys Tyr
110 115 120Leu Leu Ser Ser Ile Phe Gly Lys Gln Leu Val Val Ser Tyr
Phe 125 130 135Pro Asp Lys Val Ala Leu Leu Gln Arg Lys Val Glu Glu
Asn Arg 140 145 150Asn Ser Leu Phe Phe Phe Leu Leu Phe Leu Arg Leu
Phe Pro Met 155 160 165Thr Pro Asn Trp Phe Leu Asn Leu Ser Ala Pro
Ile Leu Asn Ile 170 175 180Pro Ile Val Gln Phe Phe Phe Ser Val Leu
Ile Gly Leu Ile Pro 185 190 195Tyr Asn Phe Ile Cys Val Gln Thr Gly
Ser Ile Leu Ser Thr Leu 200 205 210Thr Ser Leu Asp Ala Leu Phe Ser
Trp Asp Thr Val Phe Lys Leu 215 220 225Leu Ala Ile Ala Met Val Ala
Leu Ile Pro Gly Thr Leu Ile Lys 230 235 240Lys Phe Ser Gln Lys His
Leu Gln Leu Asn Glu Thr Ser Thr Ala 245 250 255Asn His Ile His Ser
Arg Lys Asp Thr 26067339PRTHomo sapiensmisc_featureIncyte ID No
2493824 67Met Ala Ala Ala Cys Gly Pro Gly Ala Ala Gly Tyr Cys Leu
Leu1 5 10 15Leu Gly Leu His Leu Phe Leu Leu Thr Ala Gly Pro Ala Leu
Gly 20 25 30Trp Asn Asp Pro Asp Arg Met Leu Leu Arg Asp Val Lys Ala
Leu 35 40 45Thr Leu His Tyr Asp Arg Tyr Thr Thr Ser Arg Arg Leu Asp
Pro 50 55 60Ile Pro Gln Leu Lys Cys Val Gly Gly Thr Ala Gly Cys Asp
Ser 65 70 75Tyr Thr Pro Lys Val Ile Gln Cys Gln Asn Lys Gly Trp Asp
Gly 80 85 90Tyr Asp Val Gln Trp Glu Cys Lys Thr Asp Leu Asp Ile Ala
Tyr 95 100 105Lys Phe Gly Lys Thr Val Val Ser Cys Glu Gly Tyr Glu
Ser Ser 110 115 120Glu Asp Gln Tyr Val Leu Arg Gly Ser Cys Gly Leu
Glu Tyr Asn 125 130 135Leu Asp Tyr Thr Glu Leu Gly Leu Gln Lys Leu
Lys Glu Ser Gly 140 145 150Lys Gln His Gly Phe Ala Ser Phe Ser Asp
Tyr Tyr Tyr Lys Trp 155 160 165Ser Ser Ala Asp Ser Cys Asn Met Ser
Gly Leu Ile Thr Ile Val 170 175 180Val Leu Leu Gly Ile Ala Phe Val
Val Tyr Lys Leu Phe Leu Ser 185 190 195Asp Gly Gln Tyr Ser Pro Pro
Pro Tyr Ser Glu Tyr Pro Pro Phe 200 205 210Ser His Arg Tyr Gln Arg
Phe Thr Asn Ser Ala Gly Pro Pro Pro 215 220 225Pro Gly Phe Lys Ser
Glu Phe Thr Gly Pro Gln Asn Thr Gly His 230 235 240Gly Ala Thr Ser
Gly Phe Gly Ser Ala Phe Thr Gly Gln Gln Gly 245 250 255Tyr Glu Asn
Ser Gly Pro Gly Phe Trp Thr Gly Leu Gly Thr Gly 260 265 270Gly Ile
Leu Gly Tyr Leu Phe Gly Ser Asn Arg Ala Ala Thr Pro 275 280 285Phe
Ser Asp Ser Trp Tyr Tyr Pro Ser Tyr Pro Pro Ser Tyr Pro 290 295
300Gly Thr Trp
Asn Arg Ala Tyr Ser Pro Leu His Gly Gly Ser Gly 305 310 315Ser Tyr
Ser Val Cys Ser Asn Ser Asp Thr Lys Thr Arg Thr Ala 320 325 330Ser
Gly Tyr Gly Gly Thr Arg Arg Arg 33568397PRTHomo
sapiensmisc_featureIncyte ID No 2555823 68Met Val Arg Pro Gly Ala
Arg Leu Cys Leu Gly Ser Val Gly Arg1 5 10 15Gly Leu Cys Leu Val Leu
Pro Leu Leu Cys Leu Gly Ala Gly Phe 20 25 30Leu Phe Leu Asn Thr Leu
Phe Ile Gln Arg Gly Arg His Glu Thr 35 40 45Thr Trp Thr Ile Leu Arg
Arg Phe Gly Tyr Ser Asp Ala Leu Glu 50 55 60Leu Thr Ala Asp Tyr Leu
Ser Pro Leu Ile His Val Pro Pro Gly 65 70 75Cys Ser Thr Glu Leu Asn
His Leu Gly Tyr Gln Phe Val Gln Arg 80 85 90Val Phe Glu Lys His Asp
Gln Asp Arg Asp Gly Ala Leu Ser Pro 95 100 105Val Glu Leu Gln Ser
Leu Phe Ser Val Phe Pro Ala Ala Pro Trp 110 115 120Gly Pro Glu Leu
Pro Arg Thr Val Arg Thr Glu Ala Gly Arg Leu 125 130 135Pro Leu His
Gly Tyr Leu Cys Gln Trp Thr Leu Val Thr Tyr Leu 140 145 150Asp Val
Arg Ser Cys Leu Gly His Leu Gly Tyr Leu Gly Tyr Pro 155 160 165Thr
Leu Cys Glu Gln Asp Gln Ala His Ala Ile Thr Val Thr Arg 170 175
180Glu Lys Arg Leu Asp Gln Glu Lys Gly Gln Thr Gln Arg Ser Val 185
190 195Leu Leu Cys Lys Val Val Gly Ala Arg Gly Val Gly Lys Ser Ala
200 205 210Phe Leu Gln Ala Phe Leu Gly Arg Gly Leu Gly His Gln Asp
Thr 215 220 225Arg Glu Gln Pro Pro Gly Tyr Ala Ile Asp Thr Val Gln
Val Asn 230 235 240Gly Gln Glu Lys Tyr Leu Ile Leu Cys Glu Val Gly
Thr Asp Gly 245 250 255Leu Leu Ala Thr Ser Leu Asp Ala Thr Cys Asp
Val Ala Cys Leu 260 265 270Met Phe Asp Gly Ser Asp Pro Lys Ser Phe
Ala His Cys Ala Ser 275 280 285Val Tyr Lys His His Tyr Met Asp Gly
Gln Thr Pro Cys Leu Phe 290 295 300Val Ser Ser Lys Ala Asp Leu Pro
Glu Gly Val Ala Val Ser Gly 305 310 315Pro Ser Pro Ala Glu Phe Cys
Arg Lys His Arg Leu Pro Ala Pro 320 325 330Val Pro Phe Ser Cys Ala
Gly Pro Ala Glu Pro Ser Thr Thr Ile 335 340 345Phe Thr Gln Leu Ala
Thr Met Ala Ala Phe Pro His Leu Val His 350 355 360Ala Glu Leu His
Pro Ser Ser Phe Trp Leu Arg Gly Leu Leu Gly 365 370 375Val Val Gly
Ala Ala Val Ala Ala Val Leu Ser Phe Ser Leu Tyr 380 385 390Arg Val
Leu Val Lys Ser Gln 39569301PRTHomo sapiensmisc_featureIncyte ID No
2598242 69Met Glu Leu Ser Asp Val Thr Leu Ile Glu Gly Val Gly Asn
Glu1 5 10 15Val Met Val Val Ala Gly Val Val Val Leu Ile Leu Ala Leu
Val 20 25 30Leu Ala Trp Leu Ser Thr Tyr Val Ala Asp Ser Gly Ser Asn
Gln 35 40 45Leu Leu Gly Ala Ile Val Ser Ala Gly Asp Thr Ser Val Leu
His 50 55 60Leu Gly His Val Asp His Leu Val Ala Gly Gln Gly Asn Pro
Glu 65 70 75Pro Thr Glu Leu Pro His Pro Ser Glu Gly Asn Asp Glu Lys
Ala 80 85 90Glu Glu Ala Gly Glu Gly Arg Gly Asp Ser Thr Gly Glu Ala
Gly 95 100 105Ala Gly Gly Gly Val Glu Pro Ser Leu Glu His Leu Leu
Asp Ile 110 115 120Gln Gly Leu Pro Lys Arg Gln Ala Gly Ala Gly Ser
Ser Ser Pro 125 130 135Glu Ala Pro Leu Arg Ser Glu Asp Ser Thr Cys
Leu Pro Pro Ser 140 145 150Pro Gly Leu Ile Thr Val Arg Leu Lys Phe
Leu Asn Asp Thr Glu 155 160 165Glu Leu Ala Val Ala Arg Pro Glu Asp
Thr Val Gly Ala Leu Lys 170 175 180Ser Lys Tyr Phe Pro Gly Gln Glu
Ser Gln Met Lys Leu Ile Tyr 185 190 195Gln Gly Arg Leu Leu Gln Asp
Pro Ala Arg Thr Leu Arg Ser Leu 200 205 210Asn Ile Thr Asp Asn Cys
Val Ile His Cys His Arg Ser Pro Pro 215 220 225Gly Ser Ala Val Pro
Gly Pro Ser Ala Ser Leu Ala Pro Ser Ala 230 235 240Thr Glu Pro Pro
Ser Leu Gly Val Asn Val Gly Ser Leu Met Val 245 250 255Pro Val Phe
Val Val Leu Leu Gly Val Val Trp Tyr Phe Arg Ile 260 265 270Asn Tyr
Arg Gln Phe Phe Thr Ala Pro Ala Thr Val Ser Leu Val 275 280 285Gly
Val Thr Val Phe Phe Ser Phe Leu Val Phe Gly Met Tyr Gly 290 295
300Arg 70217PRTHomo sapiensmisc_featureIncyte ID No 2634120 70Met
Val Glu Val Gln Leu Glu Ser Asp His Glu Tyr Pro Pro Gly1 5 10 15Leu
Leu Val Ala Phe Ser Ala Cys Thr Thr Val Leu Val Ala Val 20 25 30His
Leu Phe Ala Leu Met Val Ser Thr Cys Leu Leu Pro His Ile 35 40 45Glu
Ala Val Ser Asn Ile His Asn Leu Asn Ser Val His Gln Ser 50 55 60Pro
His Gln Arg Leu His Arg Tyr Val Glu Leu Ala Trp Gly Phe 65 70 75Ser
Thr Ala Leu Gly Thr Phe Leu Phe Leu Ala Glu Val Val Leu 80 85 90Val
Gly Trp Val Lys Phe Val Pro Ile Gly Ala Pro Leu Asp Thr 95 100
105Pro Thr Pro Met Val Pro Thr Ser Arg Val Pro Gly Thr Leu Ala 110
115 120Pro Val Ala Thr Ser Leu Ser Pro Ala Ser Asn Leu Pro Arg Ser
125 130 135Ser Ala Ser Ala Ala Pro Ser Gln Ala Glu Pro Ala Cys Pro
Pro 140 145 150Arg Gln Ala Cys Gly Gly Gly Gly Ala His Gly Pro Gly
Trp Gln 155 160 165Ala Ala Met Ala Ser Thr Ala Ile Met Val Pro Val
Gly Leu Val 170 175 180Phe Val Ala Phe Ala Leu His Phe Tyr Arg Ser
Leu Val Ala His 185 190 195Lys Thr Asp Arg Tyr Lys Gln Glu Leu Glu
Glu Leu Asn Arg Leu 200 205 210Gln Gly Glu Leu Gln Ala Val
21571143PRTHomo sapiensmisc_featureIncyte ID No 2765411 71Met Phe
Pro Val Leu Gly Trp Ile Leu Ile Ala Val Val Ile Ile1 5 10 15Ile Leu
Leu Ile Phe Thr Ser Val Thr Arg Cys Leu Ser Pro Val 20 25 30Ser Phe
Leu Gln Leu Lys Phe Trp Lys Ile Tyr Leu Glu Gln Glu 35 40 45Gln Gln
Ile Leu Lys Ser Lys Ala Thr Glu His Ala Thr Glu Leu 50 55 60Ala Lys
Glu Asn Ile Lys Cys Phe Phe Glu Gly Ser His Pro Lys 65 70 75Glu Tyr
Asn Thr Pro Ser Met Lys Glu Trp Gln Gln Ile Ser Ser 80 85 90Leu Tyr
Thr Phe Asn Pro Lys Gly Gln Tyr Tyr Ser Met Leu His 95 100 105Lys
Tyr Val Asn Arg Lys Glu Lys Thr His Ser Ile Arg Ser Thr 110 115
120Glu Gly Asp Thr Val Ile Pro Val Leu Gly Phe Val Asp Ser Ser 125
130 135Gly Ile Asn Ser Thr Pro Glu Leu 14072186PRTHomo
sapiensmisc_featureIncyte ID No 2769412 72Met Ser Gly Ile Ser Gly
Cys Pro Phe Phe Leu Trp Gly Leu Leu1 5 10 15Ala Leu Leu Gly Leu Ala
Leu Val Ile Ser Leu Ile Phe Asn Ile 20 25 30Ser His Tyr Val Glu Lys
Gln Arg Gln Asp Lys Met Tyr Ser Tyr 35 40 45Ser Ser Asp His Thr Arg
Val Asp Glu Tyr Tyr Ile Glu Asp Thr 50 55 60Pro Ile Tyr Gly Asn Leu
Asp Asp Met Ile Ser Glu Pro Met Asp 65 70 75Glu Asn Cys Tyr Glu Gln
Met Lys Ala Arg Pro Glu Lys Ser Val 80 85 90Asn Lys Met Gln Glu Ala
Thr Pro Ser Ala Gln Ala Thr Asn Glu 95 100 105Thr Gln Met Cys Tyr
Ala Ser Leu Asp His Ser Val Lys Gly Lys 110 115 120Arg Arg Lys Pro
Arg Lys Gln Asn Thr His Phe Ser Asp Lys Asp 125 130 135Gly Asp Glu
Gln Leu His Ala Ile Asp Ala Ser Val Ser Lys Thr 140 145 150Thr Leu
Val Asp Ser Phe Ser Pro Glu Ser Gln Ala Val Glu Glu 155 160 165Asn
Ile His Asp Asp Pro Ile Arg Leu Phe Gly Leu Ile Arg Ala 170 175
180Lys Arg Glu Pro Ile Asn 18573364PRTHomo
sapiensmisc_featureIncyte ID No 2842779 73Met Pro Gly Cys Pro Cys
Pro Gly Cys Gly Met Ala Gly Pro Arg1 5 10 15Leu Leu Phe Leu Thr Ala
Leu Ala Leu Glu Leu Leu Gly Arg Ala 20 25 30Gly Gly Ser Gln Pro Ala
Leu Arg Ser Arg Gly Thr Ala Thr Ala 35 40 45Cys Arg Leu Asp Asn Lys
Glu Ser Glu Ser Trp Gly Ala Leu Leu 50 55 60Ser Gly Glu Arg Leu Asp
Thr Trp Ile Cys Ser Leu Leu Gly Ser 65 70 75Leu Met Val Gly Leu Ser
Gly Val Phe Pro Leu Leu Val Ile Pro 80 85 90Leu Glu Met Gly Thr Met
Leu Arg Ser Glu Ala Gly Ala Trp Arg 95 100 105Leu Lys Gln Leu Leu
Ser Phe Ala Leu Gly Gly Leu Leu Gly Asn 110 115 120Val Phe Leu His
Leu Leu Pro Glu Ala Trp Ala Tyr Thr Cys Ser 125 130 135Ala Ser Pro
Gly Gly Glu Gly Gln Ser Leu Gln Gln Gln Gln Gln 140 145 150Leu Gly
Leu Trp Val Ile Ala Gly Ile Leu Thr Phe Leu Ala Leu 155 160 165Glu
Lys Met Phe Leu Asp Ser Lys Glu Glu Gly Thr Ser Gln Ala 170 175
180Pro Asn Lys Asp Pro Thr Ala Ala Ala Ala Ala Leu Asn Gly Gly 185
190 195His Cys Leu Ala Gln Pro Ala Ala Glu Pro Gly Leu Gly Ala Val
200 205 210Val Arg Ser Ile Lys Val Ser Gly Tyr Leu Asn Leu Leu Ala
Asn 215 220 225Thr Ile Asp Asn Phe Thr His Gly Leu Ala Val Ala Ala
Ser Phe 230 235 240Leu Val Ser Lys Lys Ile Gly Leu Leu Thr Thr Met
Ala Ile Leu 245 250 255Leu His Glu Ile Pro His Glu Val Gly Asp Phe
Ala Ile Leu Leu 260 265 270Arg Ala Gly Phe Asp Arg Trp Ser Ala Ala
Lys Leu Gln Leu Ser 275 280 285Thr Ala Leu Gly Gly Leu Leu Gly Ala
Gly Phe Ala Ile Cys Thr 290 295 300Gln Ser Pro Lys Gly Val Glu Glu
Thr Ala Ala Trp Val Leu Pro 305 310 315Phe Thr Ser Gly Gly Phe Leu
Tyr Ile Ala Leu Val Asn Val Leu 320 325 330Pro Asp Leu Leu Glu Glu
Glu Asp Pro Trp Arg Ser Leu Gln Gln 335 340 345Leu Leu Leu Leu Cys
Ala Gly Ile Val Val Met Val Leu Phe Ser 350 355 360Leu Phe Val Asp
74605PRTHomo sapiensmisc_featureIncyte ID No 2966260 74Met Gly Arg
Leu Leu Arg Ala Ala Arg Leu Pro Pro Leu Leu Ser1 5 10 15Pro Leu Leu
Leu Leu Leu Val Gly Gly Ala Phe Leu Gly Ala Cys 20 25 30Val Ala Gly
Ser Asp Glu Pro Gly Pro Glu Gly Leu Thr Ser Thr 35 40 45Ser Leu Leu
Asp Leu Leu Leu Pro Thr Gly Leu Glu Pro Leu Asp 50 55 60Ser Glu Glu
Pro Ser Glu Thr Met Gly Leu Gly Ala Gly Leu Gly 65 70 75Ala Pro Gly
Ser Gly Phe Pro Ser Glu Glu Asn Glu Glu Ser Arg 80 85 90Ile Leu Gln
Pro Pro Gln Tyr Phe Trp Glu Glu Glu Glu Glu Leu 95 100 105Asn Asp
Ser Ser Leu Asp Leu Gly Pro Thr Ala Asp Tyr Val Phe 110 115 120Pro
Asp Leu Thr Glu Lys Ala Gly Ser Ile Glu Asp Thr Ser Gln 125 130
135Ala Gln Glu Leu Pro Asn Leu Pro Ser Pro Leu Pro Lys Met Asn 140
145 150Leu Val Glu Pro Pro Trp His Met Pro Pro Arg Glu Glu Glu Glu
155 160 165Glu Glu Glu Glu Glu Glu Glu Met Glu Lys Glu Glu Val Glu
Lys 170 175 180Gln Asp Val Glu Glu Glu Glu Glu Leu Leu Pro Val Asn
Gly Ser 185 190 195Gln Glu Glu Ala Lys Pro Gln Val Arg Asp Phe Ser
Leu Thr Ser 200 205 210Ser Ser Gln Thr Pro Gly Ala Thr Lys Ser Arg
His Glu Asp Ser 215 220 225Gly Asp Gln Ala Ser Ser Gly Val Glu Val
Glu Ser Ser Met Gly 230 235 240Pro Ser Leu Leu Leu Pro Ser Val Thr
Pro Thr Ile Val Thr Pro 245 250 255Gly Asp Gln Asp Ser Thr Ser Gln
Glu Ala Glu Ala Thr Val Leu 260 265 270Pro Ala Ala Gly Leu Gly Val
Glu Phe Glu Ala Pro Gln Glu Ala 275 280 285Ser Glu Glu Ala Thr Ala
Gly Ala Ala Gly Leu Ser Gly Gln His 290 295 300Glu Glu Val Pro Ala
Leu Pro Ser Phe Pro Gln Thr Thr Ala Pro 305 310 315Ser Gly Ala Glu
His Pro Asp Glu Asp Pro Leu Gly Ser Arg Thr 320 325 330Ser Ala Ser
Ser Pro Leu Ala Pro Gly Asp Met Glu Leu Thr Pro 335 340 345Ser Ser
Ala Thr Leu Gly Gln Glu Asp Leu Asn Gln Gln Leu Leu 350 355 360Glu
Gly Gln Ala Ala Glu Ala Gln Ser Arg Ile Pro Trp Asp Ser 365 370
375Thr Gln Val Ile Cys Lys Asp Trp Ser Asn Leu Ala Gly Lys Asn 380
385 390Tyr Ile Ile Leu Asn Met Thr Glu Asn Ile Asp Cys Glu Val Phe
395 400 405Arg Gln His Arg Gly Pro Gln Leu Leu Ala Leu Val Glu Glu
Val 410 415 420Leu Pro Arg His Gly Ser Gly His His Gly Ala Trp His
Ile Ser 425 430 435Leu Ser Lys Pro Ser Glu Lys Glu Gln His Leu Leu
Met Thr Leu 440 445 450Val Gly Glu Gln Gly Val Val Pro Thr Gln Asp
Val Leu Ser Met 455 460 465Leu Gly Asp Ile Arg Arg Ser Leu Glu Glu
Ile Gly Ile Gln Asn 470 475 480Tyr Ser Thr Thr Ser Ser Cys Gln Ala
Arg Ala Ser Gln Val Arg 485 490 495Ser Asp Tyr Gly Thr Leu Phe Val
Val Leu Val Val Ile Gly Ala 500 505 510Ile Cys Ile Ile Ile Ile Ala
Leu Gly Leu Leu Tyr Asn Cys Trp 515 520 525Gln Arg Arg Leu Pro Lys
Leu Lys His Val Ser His Gly Glu Glu 530 535 540Leu Arg Phe Val Glu
Asn Gly Cys His Asp Asn Pro Thr Leu Asp 545 550 555Val Ala Ser Asp
Ser Gln Ser Glu Met Gln Glu Lys His Pro Ser 560 565 570Leu Asn Gly
Gly Gly Ala Leu Asn Gly Pro Gly Ser Trp Gly Ala 575 580 585Leu Met
Gly Gly Lys Arg Asp Pro Glu Asp Ser Asp Val Phe Glu 590 595 600Glu
Asp Thr His Leu 6057597PRTHomo sapiensmisc_featureIncyte ID No
2993326 75Met Thr Gly Arg Phe Lys Ala Cys Gln Val Ile Leu Gly Leu
Leu1 5 10 15Val Ala Ile Ser Leu Ala Ala Gly Thr Gly Gly Ala Ala Gly
Ala 20 25
30Ala Leu Val Ile Val Phe Ile Gly Ala Phe Leu Val Leu Leu Phe 35 40
45Leu Gly Arg Leu Thr Thr Gly Gly Ser Met Ala Arg Glu Ser Leu 50 55
60Val Ala Ala Asn Arg Val Cys Ile Ser Arg Thr Leu Ser Ser Ser 65 70
75Val Val Ser Val Cys Ile Ser Gly Gly Lys Gly Ser Pro Arg Leu 80 85
90Pro Gly Gly Gly Arg Gly Pro 9576247PRTHomo
sapiensmisc_featureIncyte ID No 3001124 76Met Val Thr Leu Val Ser
Asp Thr Ala Met Thr Pro Ile Ala Ser1 5 10 15Val Asp Thr Ile Ala Val
Cys Leu Phe Ala Gly Ala Trp Gly Gly 20 25 30Ala Met Val Pro Met His
Leu Leu Gly Arg Leu Glu Lys Pro Leu 35 40 45Leu Leu Leu Cys Cys Ala
Ser Phe Leu Leu Gly Leu Ala Leu Leu 50 55 60Gly Ile Lys Thr Asp Ile
Thr Pro Val Ala Tyr Phe Phe Leu Thr 65 70 75Leu Gly Gly Phe Phe Leu
Phe Ala Tyr Leu Leu Val Arg Phe Leu 80 85 90Glu Trp Gly Leu Arg Ser
Gln Leu Gln Ser Met Gln Thr Glu Ser 95 100 105Pro Gly Pro Ser Gly
Asn Ala Arg Asp Asn Glu Ala Phe Glu Val 110 115 120Pro Val Tyr Glu
Glu Ala Val Val Gly Leu Glu Ser Gln Cys Arg 125 130 135Pro Gln Glu
Leu Asp Gln Pro Pro Pro Tyr Ser Thr Val Val Ile 140 145 150Pro Pro
Ala Pro Glu Glu Glu Gln Pro Ser His Pro Glu Gly Ser 155 160 165Arg
Arg Ala Lys Leu Glu Gln Arg Arg Met Ala Ser Glu Gly Ser 170 175
180Met Ala Gln Glu Gly Ser Pro Gly Arg Ala Pro Ile Asn Leu Arg 185
190 195Leu Arg Gly Pro Arg Ala Val Ser Thr Ala Pro Asp Leu Gln Ser
200 205 210Leu Ala Ala Val Pro Thr Leu Glu Pro Leu Thr Pro Pro Pro
Ala 215 220 225Tyr Asp Val Cys Phe Gly His Pro Asp Asp Asp Ser Val
Phe Tyr 230 235 240Glu Asp Asn Trp Ala Pro Pro 24577193PRTHomo
sapiensmisc_featureIncyte ID No 3120070 77Met Ile Arg Cys Gly Leu
Ala Cys Glu Arg Cys Arg Trp Ile Leu1 5 10 15Pro Leu Leu Leu Leu Ser
Ala Ile Ala Phe Asp Ile Ile Ala Leu 20 25 30Ala Gly Arg Gly Trp Leu
Gln Ser Ser Asp His Gly Gln Thr Ser 35 40 45Ser Leu Trp Trp Lys Cys
Ser Gln Glu Gly Gly Gly Ser Gly Ser 50 55 60Tyr Glu Glu Gly Cys Gln
Ser Leu Met Glu Tyr Ala Trp Gly Arg 65 70 75Ala Ala Ala Ala Met Leu
Phe Cys Gly Phe Ile Ile Leu Val Ile 80 85 90Cys Phe Ile Leu Ser Phe
Phe Ala Leu Cys Gly Pro Gln Met Leu 95 100 105Val Phe Leu Arg Val
Ile Gly Gly Leu Leu Ala Leu Ala Ala Val 110 115 120Phe Gln Ile Ile
Ser Leu Val Ile Tyr Pro Val Lys Tyr Thr Gln 125 130 135Thr Phe Thr
Leu His Ala Asn Pro Ala Val Thr Tyr Ile Tyr Asn 140 145 150Trp Ala
Tyr Gly Phe Gly Trp Ala Ala Thr Ile Ile Leu Ile Gly 155 160 165Cys
Ala Phe Phe Phe Cys Cys Leu Pro Asn Tyr Glu Asp Asp Leu 170 175
180Leu Gly Asn Ala Lys Pro Arg Tyr Phe Tyr Thr Ser Ala 185
19078128PRTHomo sapiensmisc_featureIncyte ID No 3133035 78Met Asn
Met Lys Gln Lys Ser Val Tyr Gln Gln Thr Lys Ala Leu1 5 10 15Leu Cys
Lys Asn Phe Leu Lys Lys Trp Arg Met Lys Arg Glu Ser 20 25 30Leu Leu
Glu Trp Gly Leu Ser Ile Leu Leu Gly Leu Cys Ile Ala 35 40 45Leu Phe
Ser Ser Ser Met Arg Asn Val Gln Phe Pro Gly Met Ala 50 55 60Pro Gln
Asn Leu Gly Arg Val Asp Lys Phe Asn Ser Ser Ser Leu 65 70 75Met Val
Val Tyr Thr Pro Ile Ser Asn Leu Thr Gln Gln Ile Met 80 85 90Asn Lys
Thr Ala Leu Ala Pro Leu Leu Lys Gly Thr Ser Val Ile 95 100 105Gly
Ala Gln Ile Ile His Thr Trp Thr Lys Tyr Phe Trp Lys Ile 110 115
120Tyr Ile Cys Tyr Gly Asn His Leu 12579115PRTHomo
sapiensmisc_featureIncyte ID No 3436879 79Met Ala Val Ala Val Leu
Leu Cys Gly Cys Ile Val Ala Thr Val1 5 10 15Ser Phe Phe Trp Glu Glu
Ser Leu Thr Gln His Val Ala Gly Leu 20 25 30Leu Phe Leu Met Thr Gly
Ile Phe Cys Thr Ile Ser Leu Cys Thr 35 40 45Tyr Ala Ala Ser Ile Ser
Tyr Asp Leu Asn Arg Leu Pro Lys Leu 50 55 60Ile Tyr Ser Leu Pro Ala
Asp Val Glu His Gly Tyr Ser Trp Ser 65 70 75Ile Phe Cys Ala Trp Cys
Ser Leu Gly Phe Ile Val Ala Ala Gly 80 85 90Gly Leu Cys Ile Ala Tyr
Pro Phe Ile Ser Arg Thr Lys Ile Ala 95 100 105Gln Leu Lys Ser Gly
Arg Asp Ser Thr Val 110 115801869DNAHomo sapiensmisc_featureIncyte
ID No 153831 80gcgagcggct ggcggatccg acgcgcgaga ccgggagggg
acgagggcgt tgcaatcgtt 60cggggcgggg gctttccggg gagggggtgc tcaggtgcac
cagcggcggc ggaccctcag 120actctgccct cccctccctt taaccccctt
ccagccggac gggaggcggg gcagggctga 180gcatttgtga cacctacatt
tccgtggctc ccttcttttc ccccgacccc tgtttatctc 240ttcgccttcc
agaagttctt ttccatcagg ccgtcgcacc ttgcgtggga aggagcaccc
300cacttggaag caggaggcgg ggttcagatc ttggccctac ccctcctgtg
ttaaagtccg 360cgagcctcag tttccctcac agtatttttt gcctcgcctt
acccggtttt gaggatctgt 420acgagaaaga gaaaggaagt ggacatttgt
tgaattcctg catggccaaa taccacgcag 480actgcttcat ccgccacgtt
taatccttat tacttggtgt tctcagaact cccatttcat 540ggattcttaa
gctcacagag tcagtgaata acagaaaggg attcagatct agccgtttag
600ctgcacagtg gagttcttct ccagagtctt cccttgtctg ggctctggct
ggaactattc 660ctcagccaaa tcctcgcccc agaacagtgc ttcctgtttc
tccagctgag aagtctccct 720ttcagtttcc ttcttccagc acggagtaca
ctgctctgcc tccacttaga ttacttcaga 780aatgaaatgc agcaaatatt
tatccagcag tgcagggagt tgaacttttg gagtcgggaa 840ccttggattc
ttgttctggc tctgccactt actgtgtggc cttgggaagt cctttgtctt
900ctctgagctt tcttttctct ttgcgtaaaa gcggtgctct tgtcccattc
tccctccctg 960tcttccagca ggctctcccc ggaggctcag ccccctctgc
tccccatggg caactgccag 1020gcagggcaca acctgcacct gtgtctggcc
caccacccac ctctggtctg tgccactttg 1080atcctgctgc tccttggcct
ctctggcctg ggccttggca gcttcctcct cacccacagg 1140actggcctgc
gcagccctga catcccccag gactgggtct cttttttgag atcttttggc
1200cagctgaccc tgtgtcccag gaatgggaca gtcacaggga agtggcgagg
gtctcacgtc 1260gtgggcttgc tgaccacctt gaacttcgga gacggtccag
acaggaacaa gacccggaca 1320ttccaggcca cagtcctggg aagtcagatg
ggattgaaag gatcttctgc aggacaactg 1380gtccttatca cagccagggt
gaccacagaa aggactgcag gaacctgcct atattttagt 1440gctgttccag
gaatcctacc ctccagccag ccacccatat cctgctcaga ggagggggct
1500ggaaatgcca ccctgagccc tagaatgggt gaggaatgtg ttagtgtctg
gagccatgaa 1560ggccttgtgc tgaccaagct gctcacctcg gaggagctgg
ctctgtgtgg ctccaggctg 1620ctggtcttgg gctccttcct gcttctcttc
tgtggccttc tctgctgtgt cactgctatg 1680tgcttccacc cgcgccggga
gtcccactgg tctagaaccc ggctctgagg gcactggcct 1740agttcccgac
ttgtttctca ggtgtgaatc aacttcttgg gccttggctc tgagttggaa
1800aaggttttag aaaaagtgaa gagctggaat gtgggggaaa ataaaaagct
tttttgccca 1860aaaaaaaaa 1869811044DNAHomo
sapiensmisc_featureIncyte ID No 350629 81tgcagttaac atctgcacac
ttcactatat tttaagtttt tgttaatata aaagaataag 60aaaacagaaa agtattactg
ttaaacaata atagagaaat gtatacttta tttacaaatt 120tctccctcta
gctgatcata cagttgacca gttcagggtg cccgctgctg gttggatgcc
180aggcggaatg tcagggtgtt ctctggtgtc tgttgtggct gtgggatcca
cggttactgg 240gcggagcctg tggtggctgt ggtgccatgg aggggctgcg
atcttctgtg gagctggacc 300ctgagctgac tccagggaag ctggatgagg
agatggtggg gctgccaccc catgacgcga 360gtcctcaagt cactttccac
agcctcgatg ggaagacagt ggtgtgtcca cacttcatgg 420gcttactgct
gggtctctta cttttattga ctttgtctgt taggaaccaa ctctgtgtaa
480gaggtgaaag gcagcttgca gaaacactgc attcacaggt gaaggagaaa
tcccagctca 540ttggcaagaa aacagattgt agagactgag gcatctttaa
aagatgtcag ggtacagaaa 600aagtctttca acacccccgg ctttgtagat
gcctacaaga aggtgaatag caccaacgag 660atgctgatgg agaaatttac
caccctcgtt caagaactga aagaagagac atcctccaga 720ctctcctcaa
tgggcggtgc ctccaaatct aaagaatatg gaggtcctgg agcacaccaa
780gaaatgaggg actttttctt tgcagaaagt ttgaattctg tcttaatgag
acagaatgcc 840atacttgagc acctcatctt ttgctcaaat tgaaatgtca
tcgaactgta tttctcaagt 900caatggtctg taaatatgat ttatgtatta
atctcctaag tgaacaattt atattttatc 960ctctacataa ttatcgtatt
atgctttaaa tatatattta gtttatcaat aaagacattc 1020agtactcaat
agcaaaaaaa aaaa 1044823079DNAHomo sapiensmisc_featureIncyte ID No
729171 82cggctcgagg tcggctggag tcggaggcga tatttctagg ggtgtacttg
ttggggtcag 60ggtaagcacc agccacaaaa acctacaaaa gaagggaaat tactgtcttt
aaatattaaa 120aaaaaacaag atccatgagt gggcatcgat caacaaggaa
aagatgtgga gattcccacc 180cggagtcccc agtgggcttc gggcatatga
gtactacagg atgtgtatta aataaattgt 240ttcagttacc aacaccacca
ttgtcaagac accaactaaa gcggctagaa gaacacagat 300atcaaagtgc
tggacggtcc ctgcttgagc ccttagtgca agggtattgg gaatggctcg
360ttagaagagt tccctcctgg attgccccaa atctcatcac catcattgga
ctgtcaataa 420acatctgtac aactatttta ttagtcttct actgccctac
agctacagag caggcacctc 480tgtgggcata tattgcttgt gcctgtggcc
ttttcattta ccagtctttg gatgctattg 540gtgggaaaca ggcaagaaga
accaatagta gttctcctct gggagaactt tttgatcatg 600gctgtgattc
actatcaaca gtttttgtgg ttcttggaac ttgtatagca gtgcagctgg
660ggacaaaccc tgattggatg tttttttgtt gttttgcggg gacatttatg
ttctattgtg 720cgcactggca aacgtatgtt tctggaacat tgcgatttgg
aataattgat gtgactgaag 780tgcaaatctt cataataatc atgcatttgc
tggcagtgat gggaggacca cctttttggc 840aatctatgat tccagtgctg
aatattcaaa tgaaaatttt tcctgcactt tgtactgtag 900cagggaccat
atttcctgta acaaattact tccgtgtaat cttcacaggt ggtgttggca
960aaaatggatc aacaatagca ggaacaagtg tcctttctcc ttttctccat
attggatcag 1020tgattacatt agctgcaatg atctacaaga aatctgcagt
tcagcttttt gaaaagcatc 1080cctgtcttta tatactgaca tttggttttg
tgtctgctaa aatcactaat aagcttgtgg 1140ttgcacacat gacgaaaagt
gaaatgcatt tgcatgacac agcattcata ggtccggcac 1200ttttgtttct
ggaccagtat tttaacagct ttattgatga atatattgta ctttggattg
1260ccctggtttt ctctttcttt gatttgatcc gctactgtgt cagtgtttgc
aatcagattg 1320cgtctcacct gcacatacat gtcttcagaa tcaaggtctc
tacagctcat tctaatcatc 1380attaatgatg taattggtat ataggaacat
catgttttct gcaggaaaga aagtaacata 1440ttaaggagaa tgggggtgga
taagaacaaa tataatttat aataatcaat gttgtataac 1500ttttattctt
tattattggt aacacgccct aactatcctg tgtgagaatg ggaatttcaa
1560gtcccatctt gtaaattgta tatgttgtca tgcagggttt gggccaagaa
agcatgcaga 1620aaaaaatgcc atgtgattgt aattatcctg gattcagaat
aatactgtga tggggagcca 1680gatccgcagt ggtggagagt tctaatgttg
actgtttgca ggccaaaaga tgattgcttt 1740ataattttaa caaatcattg
tcttttagta acatccttgt ttagtgtctt ctcaagcttt 1800ctttactgag
gaattcagct tgtgacacag atacatccca ctagcttgtg aggtggaact
1860agtaataaag accttgaatt tggattgaaa agtttcctat ctttacattg
ttgaggaagt 1920cctttttttt tttttttttt tttttaattg ctcaagaaat
gattctctca caggcttggg 1980aaatcctgtt agcatgcaga ataatgtggt
aactttgtca atttcccatt ttattttttt 2040aaataaatat atgatctaaa
agccaacttt ttctcagttt tactcagtgg aaagataaac 2100taagttttaa
tgttattttt ttaaatttaa gcaaaattta tttctgttct ttaataaata
2160agaaaatgtg gtccactgca ttgttgtgat gtgtcttgtg acatttctat
tttgtagaaa 2220ctttaaaaag gagaactatg ttcatttttc ctgtcaatgg
tttttttgtg ttgtagttgt 2280cacctgtgtg attatcaatc atttagaaat
ctcataccct tcccctaaat tttcagcaag 2340tgcctgggcc tctctaagag
gtcactttgt actctccttt tctggcagtc tcctctttgg 2400tatctgtact
atcgtttgaa atgggaacca gatatgtttc cattttatac agataattca
2460gttgcttgaa gaagagggac acaggagaaa agatttaaac tattggctaa
aatgaggtgt 2520cttattattg attttcatct atatcttgtc ccataatcag
gaataaacag tagctacact 2580gccttgtatg gcagccagag cgctgcttgc
ttgcactttt aatgattcca tcaataccat 2640gtagattgaa ttagcaagga
gaagtaaacc tttcatttct ttgccagact atattgggaa 2700atgaaaatcc
gtcattactt ttccttgcta gcaattgttc gaatatctgg gataaagaaa
2760tacatacagg aaaatgttag ggcagaccaa gtattaaaag ctaggacaga
gcaggacaaa 2820ggaggaagga taattctact tgtttggcaa agttacatca
gttgtcttac tgacacatca 2880ggtactatct atagtggaaa ttgaggcccg
gagaggttaa atggcatgcc agtgtcactt 2940gctatttttc agaacaaaaa
ttagaatcca gatctgaatc ctggtgcagt gttctctcct 3000atgccctact
gggctttagt gggctaaagt tctgaagcaa gatgttaagg gctaattgaa
3060atgcgtttat tctcctaga 3079831298DNAHomo
sapiensmisc_featureIncyte ID No 1273641 83cccggtgcct gcgggattgc
tggagagaac gcggcgatgg agccgggcag gacccagata 60aagcttgacc ccaggtacac
agcagatctt ctggaggtgc tgaagaccaa ttacggcatc 120ccctccgcct
gcttctctca gcctcccaca gcagcccact cctgagagcc ctgggccctg
180tggaacttgc cctcactagc atcctgacct tgctggcgct gggctccatt
gccatcttcc 240tggaggatgc cgtctacctg tacaagaaca ccctttgccc
catcaagagg cggactctgc 300tctggaagag ctcggcaccc acggtggtgt
ctgtgctgtg ctgctttggt ctctggatcc 360ctcgttccct ggtgctggtg
gaaatgacca tcacctcgtt ttatgccgtg tgcttttacc 420tgctgatgct
ggtcatggtg gaaggctttg gggggaagga ggcagtgctg aggacgctga
480gggacacccc gatgatggtc cacacaggcc cctgctgctg ctgctgcccc
tgctgtcaac 540ggctgctgct caccaggaag aagcttcagc tgctgatgtt
gggccctttc caatacgcct 600tcttgaagat aacgctgacc tggtgggcct
tgttctcgtc cccgacggaa tcttatgacc 660cagcagacat ttctgagggg
agcacagctc tatggatcaa cactttcctt ggcgtgtcca 720cactgctggc
tctctggacc ctgggcatca tttcccgtca agccaggcta cacctgggtg
780agcagaacat gggagccaaa tttgctctgt tccaggttct cctcatcctg
actgccctac 840agccctccat cttctcagtc ttggccaacg gtgggcagat
tgcttgttcg cctccctatt 900cctctaaaac caggtctcaa gtgatgaatt
gccacctcct catactggag acttttctaa 960tgactgtgct gacacgaatg
tactaccgaa ggaaagacca caaggttggg tatgaaactt 1020tctcttctcc
agacctggac ttgaacctca aagcctaagg tggatggctt ggacaatgaa
1080aggatgctgt actcattaga atacaagatt cctttactgt ccctcaacct
tgaccaaatg 1140ggaagcattc ccccttgtca acacaagctg gcagatacat
ttgactctac agatgaaggt 1200gaacaatgtt aggataaaat tgctttggat
cttgcctgga aggtgtttta agttttgtaa 1260taaacaagat gatgtctgaa
aatgtgaaaa aaaaaaaa 1298842106DNAHomo sapiensmisc_featureIncyte ID
No 1427389 84gtggggctgc ggccgggatt tgtcccctct tcggcttccg tagaggaagt
ggcgcggacc 60ttcatttggg gtttcggttc ccccccttcc ccttccccgg ggtctggggg
tgacattgca 120ccgcgcccct cgtggggtcg cgttgccacc ccacgcggac
tccccagctg gcgcgcccct 180cccatttgcc tgtcctggtc aggcccccac
cccccttccc acctgaccag ccatgggggc 240tgcggtgttt ttcggctgca
ctttcgtcgc gttcggcccg gccttcgcgc ttttcttgat 300cactgtggct
ggggacccgc ttcgcgttat catcctggtc gcaggggcat ttttctggct
360ggtctccctg ctcctggcct ctgtggtctg gttcatcttg gtccatgtga
ccgaccggtc 420agatgcccgg ctccagtacg gcctcctgat ttttggtgct
gctgtctctg tccttctaca 480ggaggtgttc cgctttgcct actacaagct
gcttaagaag gcagatgagg ggttagcatc 540gctgagtgag gacggaagat
cacccatctc catccgccag atggcctatg tttctggtct 600ctccttcggt
atcatcagtg gtgtcttctc tgttatcaat attttggctg atgcacttgg
660gccaggtgtg gttgggatcc atggagactc accctattac ttcctgactt
cagcctttct 720gacagcagcc attatcctgc tccatacctt ttggggagtt
gtgttctttg atgcctgtga 780gaggagacgg tactgggctt tgggcctggt
ggttgggagt cacctactga catcgggact 840gacattcctg aacccctggt
atgaggccag cctgctgccc atctatgcag tcactgtttc 900catggggctc
tgggccttca tcacagctgg agggtccctc cgaagtattc agcgcagcct
960cttgtgtaag gactgactac ctggactgat cgcctgacag atcccacctg
cctgtccact 1020gcccatgact gagcccagcc ccagcccggg tccattgccc
acattctctg tctccttctc 1080gtcggtctac cccactacct ccagggtttt
gctttgtcct tttgtgaccg ttagtctcta 1140agctttacca ggagcagcct
gggttcagcc agtcagtgac tggtgggttt gaatctgcac 1200ttatccccac
cacctgggga cccccttgtt gtgtccagga ctccccctgt gtcagtgctc
1260tgctctcacc ctgcccaaga ctcacctccc ttcccctctg caggccgacg
gcaggaggac 1320agtcgggtga tggtgtattc tgccctgcgc atcccacccg
aggactgagg gaacctaggg 1380gggacccctg ggcctggggt gccctcctga
tgtcctcgcc ctgtatttct ccatctccag 1440ttctggacag tgcaggttgc
caagaaaagg gacctagttt agccattgcc ctggagatga 1500aattaatgga
ggctcaagga tagatgagct ctgagtttct cagtactccc tcaagactgg
1560acatcttggt ctttttctca ggcctgaggg ggaaccattt ttggtgtgat
aaatacccta 1620aactgccttt ttttcttttt tgaggtgggg ggagggagga
ggtatattgg aactcttcta 1680acctccttgg gctatatttt ctctcctcga
gttgctcctc atggctgggc tcatttcggt 1740ccctttctcc ttggtcccag
accttggggg aaaggaagga agtgcatgtt tgggaactgg 1800cattactgga
actaatggtt ttaacctcct taaccaccag catccctcct ctccccaagg
1860tgaagtggag ggtgctgtgg tgagctggcc actccagagc tgcagtgcca
ctggaggagt 1920cagactacca tgacatcgta gggaaggagg ggagattttt
ttgtagtttt taattggggt 1980gtgggagggg cggggaggtt ttctataaac
tgtatcattt tctgctgagg gtggagtgtc 2040ccatcctttt aatcaaggtg
attgtgattt tgactaataa aaaagaattt gtaaaaaaaa 2100aaaaaa
210685899DNAHomo sapiensmisc_featureIncyte ID No 1458357
85gctgtattca ggtccccgat gggcatatac atcttagccg gtgatacact acctcttacg
60tgttgcctct ttgtgttgct tggtgctctt tcgaaaacaa ggtgcttatg gctttcatag
120actatttcct ttttcatctt
tgtcattctt taaaagtgta tgtactggtt acatcaagat 180atgttttggt
tgttagtact tattttaatt tgtttggtca cacacttaat aacacgtgaa
240actatttatg tgaagtcctt gttttatttt aaaattctct ttgtgtattt
ggaatcaaag 300ccagcacatt gtaacctgtg cttgtacgca aaagaattag
atttctttgt ttttgtttta 360ttttttaaat tgttgtaaaa attattatag
gccagctaca tctagtagta ggtttggggt 420acagattggg ggttgtgcca
tactgttttt aaagttcatg atcatctgga atgatactta 480gtgtatatat
attttgtaaa gttttaattc agcaaatttt ttgaaattgc tgctgtttta
540aattataaaa cctttatatt tctgctttgt agaaattata tgttttgtag
tattcattga 600ttttctttca ctgtacttaa atttagtgtt agtactttaa
aatttttaat ttaccagtct 660ttaaagcaac atccagaaaa aaaaaagtct
tttcccattt aaaataggct cagccagttc 720aatgtcgcct tgttatcaga
gaaatattag ttcaatactg aaagaaaaat attatacctc 780ttggtatcta
gaaaaacttg ttcatccatt ataaatatat ctttagccac agcaaaccac
840acttaaccta tctataataa aaatgtgctt taaataaaac caaaaaaaga aaaaaaaaa
899862000DNAHomo sapiensmisc_featureIncyte ID No 1482837
86tttgtccagt tgttcttttg ttcctttccc gcctcccatt ctgctgctct tcctccctct
60tttccccaac ctcccccgac cctcctgaat tttggaaagc acatttgcaa tattcgtgtt
120tgctttggga cggaccctcc gtttcatctc atgctttatg tgtaagagtt
ttttattatt 180attttttctt tcctttctct ctctgagaaa gttgtggctg
cgttttgatc ttaggtttta 240caaagtggtt tagggaagcg gttttgggga
gaaggatcac gaggaatgta gggaagccgg 300agggatgggt gctgctgcga
cgaccccccc gtccctcggc cccagccctc ctgccctccc 360cgtcaatctc
atccaccaaa tctgaaggcc ttaaaattgt gtgttgggag gatgtgaatt
420gggaggacgg tgtcactaga ctgtggatta gggatggtaa agtagggagg
atgctatttt 480gcaactatag taacgactta gtgttttgga aaggaaaaga
agttaaactt gaaatacgtg 540actagaacag ttgtcatgtt tataatgtga
aaagggtgaa atcatttaga ggaggggccg 600tctgtaagaa atcattatgc
actatggctt cctcctctgg tctgggaaga agcggggact 660ggctggccct
caggggatct gtaaatccca gaaaacagta tttttaacag caagatgtca
720ttcaacattg gtggggaagg aagagaaaaa aatcaaacta ttccatagaa
ctagctggcc 780ccctcactcc catgcccttc ccactcagcc tgggcccctc
cccgctccat tcataaaagc 840tgagagggtt gagctaatct tcacaaattg
taatattttt gtagtatctg ttagttcctt 900cgtcagttct gcagaacctt
gccctttcct tttgtaatgt gaataggaag acaaaagaca 960aaaaaaaaat
ccaccaccac caaaatatcc ctttgtacat gtatgtgcgt gtgcgcgtgt
1020gctttgtgtg tgtggttgtg tgttaaatca tgcagtattg tcgtaatctg
gtgttgcagc 1080aatggatggt actaaatcag cacctggatg ccccacccaa
ccccgtgggc cctgcagacc 1140ccagtaggga ggtatgggga gagctcaggg
gagtgtggtt tctgagggct actgtctggg 1200gacacctctg aacttactgt
accttcctct ccccatgaag acacctgaat agagtctaac 1260atgcctcttc
tccaacttcc tacctacaac aacagaacag ttctaatgtt gcacggccta
1320gtggccaggg ggcaagctaa gaggctgtct ggaggcttta tatgtgtctg
gagttaaggg 1380gagaggagga gggtagacag gggtctctcc ccaggtggga
tctgaatatc tgtcctcccc 1440tcttcttcat gccacctgac tccttcggcc
ccctggctgc ctttagctgt ggtactgctg 1500acaaccctgc ttgctactgc
cttatccagc acagtgaaaa acttctccag cctggcaagg 1560ccacgttggt
taatagtccc tttcccatgt ccagctccta caaatatgtc ccttaatgca
1620tttggtgaca tttacacctc actcatgtgc tctttcccta ttcactcctt
cactcattca 1680aagcattaaa atcctatgta tatataggat agacaaatat
atagatatat agatatatat 1740atatatagca agagattgat ataaaatagt
aaatatcatt gctgctttgg gctgctttgg 1800aggaggaggc catgaatatg
gggaaggcag atctggggtg caggggtagg tagggaggct 1860gggggaccca
gtgattcagt acaatccaag ggatgcaacg cgggcttgtt taatctttgt
1920gcctgaacag tttttccatg ttgagaaaac tgttcaggca cagagattaa
acagttttct 1980caacatggga aaaaaaaaaa 2000871359DNAHomo
sapiensmisc_featureIncyte ID No 1517434 87tgcccatcct gctgctcagc
ctggtcacca tgtgcgtcac gcagctgcgg ctcatcttct 60acatgggggc tatgaacaac
atcctcaagt tcctggtcag cggcgaccag aagacagttg 120gcctctacac
ctccatcttc ggcgtgctcc agctgctgtg cctgctgacg gcccccgtca
180ttggctacat catggactgg aggctgaagg agtgtgaaga cgcctccgag
gagcccgagg 240agaaagacgc caaccaaggc gagaagaaaa agaagaagcg
ggaccggcag atccagaaga 300tcactaatgc catgcgggcc ttcgccttca
ccaacctgct gctcgtgggc tttggggtga 360cctgcctcat tcccaacctg
cctctccaga tcctctcctt catcctgcac acaatcgtgc 420gaggattcat
ccactccgct gtcgggggcc tgtacgctgc cgtgtacccc tccacccagt
480tcggcagcct cacgggactg cagtctctga tcagcgcgct cttcgccctt
ctgcagcagc 540cgctgtttct ggccatgatg ggtcctctcc agggagaccc
tctgtgggtg aacgtggggc 600tgctccttct cagcctgctg ggcttctgcc
tcccgctcta cctgatctgc taccggcgcc 660agctggagcg gcagctgcag
cagaggcagg aggatgacaa actcttcctc aaaatcaacg 720gctcgtccaa
ccaggaggcc ttcgtgtagt ggctgccgcc tcggaactgc ggtctcctgc
780ctgtgcttca gtgactgacc cctgtcctgc ccctccagag taccccacgc
acccccagga 840ccttcgccgt ctccgtgcca gcgttcacgc tccctcccgg
ggccctgcct cggagctctg 900tggtggaagg acgggagagg gccccggaca
cgcgcgtttt ctcctgccga acgcaggggc 960tgccctgact ttgctctgcc
gccccccggg gacccggggc ctggggtctc tgtggtgcct 1020gcagcaggag
ccaggaacgc ccggcaggca ggcgctctcc cgccagtgtc tggattctgc
1080ctcttgccaa agcagagggg gctgccatcc cctgcctgcc acctgcccct
cggctgcatg 1140cccacagccg tacctgcctg aggacaaagg cttgcactgt
ctcgcccgcg cctggccccc 1200accccctccc cgaccagcct gatcaacatg
gtgaagcccc gtctctacta aagatacaaa 1260aattaggggg gcatggtggt
ggatgcctgt aatcccggct gctcgggagg ctgaggcgga 1320tgaatcgctt
gaaccaggag gtggaggttg cagtgaggg 1359881397DNAHomo
sapiensmisc_featureIncyte ID No 1536052 88gctggggaag ggaccatgtg
gctgccttgg gctctgttgc ttctctgggt cccagcatca 60acgtcaatga cacctgcaag
tatcactgcg gccaagacct caacaatcac aactgcattt 120ccacctgtat
catccactac cctgtttgca gtgggtgcca cccacagtgc cagcatccag
180gaggaaactg aggaggtggt gaactcacag ctcccgctgc tcctctccct
gctggcattg 240ttgctgcttc tgttggtggg ggcctccctg ctagcctgga
ggatgtttca gaaatggatc 300aaagctggtg accattcaga gctgtcccag
aaccccaagc aggcctcccc cagggaagaa 360cttcactatg cctcggtggt
gtttgattct aacaccaaca ggatagctgc tcagaggcct 420cgggaggagg
aaccagattc agattacagt gtgataagga agacataggc ttttgtcctg
480cctcgccatc ggagctctca tgggccccag gaagtccagg gacagctccc
ttatacctgg 540cccacgtcct tctcagcctg ccctcgacaa cagtgaccaa
cagacaggca gctgggtttc 600ccaggccatc cctctgttgc catcagcttg
attggcttcc ccgagggcca gcagggctgg 660gggctccgga gagcagcagg
aagcactccc agccaccagt gcctgtcacc tctttcccct 720ttgcccctgc
ttcatcccag ctctgtgtgt ggaggacaaa gcttcttcct gcgtggctcc
780aggaaaagat gtggctcacg taggtggcac ctgccaatag ctttgtcaat
cacagcccca 840taggaacgtc tggaattgct tgggagttgg ggagaactgt
caagaagagt gaagagagtg 900ccaaagcgga gatctgttca cctgggggcc
atggaggggg gacccactaa agatcaagat 960caaagattct ccccatctca
cagacaagga aactgaggcc agagggagga gagaattgct 1020catggctcca
gaactggtgg caagtttctc tggactctta ggtttatttt taatatgaaa
1080tataaaaaca gtttcaaata tcttattgag ggagaagtaa aaacttattt
aaacaataaa 1140aaaataaaaa aaaggggcgg gggtcccgag cccgaatccg
aaatcaggta aaagctgttc 1200cctgtgtaaa attgttaccc gcccaaaatt
ccacaaaata taggaccggg agccttaaag 1260tttaaacccc tgggggccca
aattgggtgg gccatccccc atttaattgg cttggccccc 1320aatggccggt
tttcacattg ggggaaacct ttttggccca acggctttta atgaaatcgg
1380cccaaaccgc gggggaa 1397891570DNAHomo sapiensmisc_featureIncyte
ID No 1666118 89atgtccatca tgtcagcagg tgcaaatcac ttttcccctt
tgcatgatct gaggcacctc 60ctcagttgtt tcactgccaa ctcttatttc agaacctgtt
tacaaacaag ccttccagtt 120ggtgaatggt tagccattgg agctcctacc
ctgtacatca gcacatcttc tggtttacaa 180gttgggtaac aatgaaagct
ggagatacta aatggaaatc cagcattgca tacccttaga 240cctgatcaca
taccagtaaa agccttaatt tagatgttag ttgtatgtgt tggacagatc
300cttgcaaaag tgtgtctgtc tattagttgt aaatttgaaa attataaatc
tctgaatctg 360ctactatcca agtttcatcc cttttgaaga tgaggcatga
gcctattaaa atatttataa 420tcatttttcg tcccctactg caagactttt
agattcttac aaatgattac tacaggaata 480gtggccactt aatgtcagtt
actccggtgg aagaatttat ctagtttttt ttcttttctt 540ttttggaagg
atggtgtgaa aaatagcaag attagagaat gagttgtata gttttttcta
600tcacatttca tctaaaatga tttgaaggac ttttgaagat ttttaccaac
atccttaaat 660caactccagg ttggatgaac aactgattta aaacaaacta
agagaacatt aactagatgt 720gggcttttta aaatatatag gtattgcatt
tcctaccttg ttatttattc cactttgaat 780actttagagg gcttaacttt
caactcttta aggtagtaat ggatagtttt atacttgttc 840tcacaaaatt
gttatggtca gtttatatca ttgctccatg cattgattat aaaaattcag
900tattaatttt ttctgatctt ataagcttta taggagtttt cttttctctt
ataaagtgtt 960tcaccttatg taaaacaaat gcctgcttgc atattggaag
atgttgaaat tagttttaga 1020caaaagtggt ccatcaattc agacactctg
cttggatgcc ttaccctttt cattagtgca 1080ttctttgctt ctgaaacttg
gcagaaactc gttagccagt ccactgcctt tctgacaatg 1140tgtggagtca
cgtatgcttg gtatatgcct ttactacttt taaagttcta cagtttatta
1200cttgcccaag tgttactaaa tccttttctt atgtgtactg gatggagaaa
aaattatagc 1260cagcactttg agaggaaagt tttcagaaac aatattaact
ggcactacta actgaaggcc 1320acaggagatg ctatcaatgt tatttgtaat
ctgaagattg aacaaggctg tgaggctcat 1380ttcaaactat tttgaggtgt
taaaatatat atatgctgtt tctcagctgt tccactcaaa 1440ccgtgttagg
actctcaaag gtaaaatgtc acaggggctt ttcagttgtt acagagctca
1500gcagctgtgg ttgcccctgt tctacaccaa tttcagttca ataaaaatgt
taactttgaa 1560aaaaaaaaaa 157090718DNAHomo
sapiensmisc_featureIncyte ID No 1675560 90ggtggtgcgt gcctgtaatc
ccaactactt gggaggctga ggcaggagaa tcgcttgaac 60ctgggaggca gcggttgcag
taagccaaga ctatgccact gcactcccgc ctgggtgaca 120aagcaatatt
ctgtgtcaaa taaataaatt cattcttctg ctctcctgac ttagagaaat
180ggtttgctta aaatgctagt aacaaacatc acagtcaaca ggagcttgct
tcatgcgaag 240gatcaatgtg atttgtggat ggagatgata gtgatgaaat
tcctgtttca tggggctgtt 300tttcttttca tctcactggg cagcaggttt
agtgaggcag tgagatgctg ctgctgtgga 360ttcttgtagc tatgcctcgg
cttcttggca tatcaggtag gaacctgtta caagtgaaat 420acttgaaacc
tctctgacca agagcctctg atggagtggg aggtgagcta attctctgac
480cagcttaggg cactgtttca gccactggtc acattccttg cttcaaactg
aaattcagtt 540tggctttgag tatagggata catggtggat tcatgtactt
cagtgtttgt tttgaccaaa 600gtttattttt ctagtgcatt ttctaagtca
aagtggtgaa aatatgtaat aattttagta 660tgcatgactc agtctgaaac
aataaaaatc tctgaaaaat gaaaaaaaaa aaaaaagg 71891904DNAHomo
sapiensmisc_featureIncyte ID No 1687323 91gcttgtgggg ggaaaaagaa
acgcaataga taaagcgggg cgcatgcgct cccggcacag 60gcttcgattg tgaggaaggc
cggctagtct ccgagctcat cccgccttgc gcatgcggag 120aaggtaaacc
agcgccccga gttgaggcgc gggtttggtg gcgcgtttca gcgaagtcgc
180acgtgaagga tagcagtggc ctgagaaaga cccagtcatg gcagcctcca
gcatcagttc 240accatgggga aagcatgtgt tcaaagccat tctgatggtc
ctagtggccc ttatcctcct 300ccactcagca ttggcccagt cccgtcgaga
ctttgcacca ccaggccaac agaagagaga 360agccccagtt gatgtcttga
cccagatagg tcgatctgtg cgagggacac tggatgcctg 420gattgggcca
gagaccatgc acctggtgtc agagtcttcg tcccaagtgt tgtgggccat
480ctcatcagcc atttctgtgg ccttctttgc tctgtctggg atcgccgcac
agctgctgaa 540tgccttggga ctagctggtg attacctcgc ccagggcctg
aagctcagcc ctggccaggt 600ccagaccttc ctgctgtggg gagcaggggc
cctggtcgtc tactggctgc tgtctctgct 660cctcggcttg gtcttggcct
tgctggggcg gatcctgtgg ggcctgaagc ttgtcatctt 720cctggccggc
ttcgtggccc tgatgaggtc ggtgcccgac ccttccaccc gggccctgct
780actcctggcc ttgctgatcc tctacgccct gctgagccgg ctcactggct
cccgagcctc 840tggggcccaa ctcgaggcca aggtgcgagg gctggaacgc
taggtggagg agctgcgctg 900gcgc 904921948DNAHomo
sapiensmisc_featureIncyte ID No 1692236 92gaagacctct tagcgggccc
atcgctgagg tgcagggaca tgtcgcggcc gaactcacct 60cgtggcctcg gcgtggtgct
ctcagctcat gcccggaaac caggtcccga cgccgcggtc 120agacggacct
ctagacgcgt ccgcctcaat gccgccagct gccaggccgc ccgtgacgcg
180ttacgcctgc gccgcctcct ggcttcgtga cgtcacgacg tccgcgcagt
gcggtcgccg 240ccgtcgcacg agtctttcct tagtaacctg ggcgatactg
tggatgtttc caaggattgt 300cttcagtcat ggccttggga ttaaagtgct
tccgcatggt ccaccctacc tttcgcaatt 360atcttgcagc ctctatcaga
cccgtttcag aagttacact gaagacagtg catgaaagac 420aacatggcca
taggcaatac atggcctatt cagctgtacc agtccgccat tttgctacca
480agaaagccaa agccaaaggg aaaggacagt cccaaaccag agtgaatatt
aatgctgcct 540tggttgagga tataatcaac ttggaagagg tgaatgaaga
aatgaagtct gtgatagaag 600ctctcaagga taatttcaat ctgactctca
atataagggc ctcaccagga tcccttgaca 660agattgctgt ggtaactgct
gacgggaagc ttgctttaaa ccagattagc cagatctcca 720tgaagtcgcc
acagctgatt ttggtgaata tggccagctt cccagagtgt acagctgcag
780ctatcaaggc tataagagaa agtggaatga atctgaaccc agaagtggaa
gggacgctaa 840ttcgggtacc cattccccaa gtaaccagag agcacagaga
aatgctggtg aaactggcca 900aacagaacac caacaaggcc aaagactctt
tacggaaggt tcgcaccaac tcaatgaaca 960agctgaagaa atccaaggat
acagtctcag aggacaccat taggctaata gagaaacaga 1020tcagccaaat
ggccgatgac acagtggcag aactggacag gcatctggca gtgaagacca
1080aagaactcct tggatgaaag tccactgggg ccagcaatac tccagagccc
agtttctgct 1140ggatcccatg ggtggcacat tgggacttct ctccctcccc
catctacaca gaagactgtc 1200accatgctga cagaagcctg tccttgtaag
gcccagcctt ccaggggaac actcagacat 1260gttcattctc ttcctgcttc
tgctctgggc cggtgggtgg ctctcagaaa atacttgctg 1320ctggcaaaag
gcctgtactc aggcatttgc tttgacttga tgttgccaag ggactgaggc
1380cattggcagg cttagtacca cctgctcctc atcttaggag tctccttttc
aaataattag 1440gctctgttcc cattttaaaa ctctgatatt ggccttcacc
tgtgactgga cactttacta 1500gaggcccatt ttcactaaac aataaaatct
aaataaattg gaaggaataa caaccacaaa 1560ggaaagaata gagttggtct
ggattgatga tcactgagga tctgtatgtg aggcacccat 1620aacagtagtt
ttgcctgtga gtcgtcttca cacatgctgt tttctctgcc tggctctctc
1680ttcccctcct tacctggcca gtcctgttta tcatcaggcc ttgtcttgga
tatcacgtcc 1740tctgggaagt cttcttttcc cctctaacct aggaccctca
ttaccggctc tcatagcaca 1800gtctactgct ttgtacgaat tctaagtatt
cttgttgcac ttaattagcc tgtatatcct 1860cagaactttg tgtaatgcct
ggagcatagt aggcagtcat atgttgtatc gtgaataaat 1920tgcacatagt
agctacccaa aaaaaaaa 194893990DNAHomo sapiensmisc_featureIncyte ID
No 1720847 93acagagactg gcacaggacc tcttcattgc aggaagatgg tagtgtaggc
aggtaacatt 60gagctctttt caaaaaagga gagctcttct tcaagataag gaagtggtag
ttatggtggt 120aacccccggc tatcagtccg gatggttgcc acccctcctg
ctgtaggatg gaagcagcca 180tggagtggga gggaggcgca ataagacacc
cctccacaga gcttggcatc atgggaagct 240ggttctacct cttcctggct
cctttgttta aaggcctggc tgggagcctt ccttttgggt 300gtctttctct
tctccaacca acagaaaaga ctgctcttca aaggtggagg gtcttcatga
360aacacagctg ccaggagccc aggcacaggg ctgggggcct ggaaaaagga
gggcacacag 420gaggagggag gagctggtag ggagatgctg gctttaccta
aggtctcgaa acaaggaggg 480cagaataggc agaggcctct ccgttccagg
cccatttttg acagatggcg ggacggaaat 540gcaatagacc agcctgcaag
aaagacatgt gttttgatga caggcagtgt ggccgggtgg 600aacaagcaca
ggccttggaa tccaatggac tgaatcagaa ccctaggcct gccatctgtc
660agccgggtga cctgggtcaa ttttagcctc taaaagcctc agtctcctta
tctgcaaaat 720gaggcttgtg atacctgttt tgaagggttg ctgagaaaat
taaagataag ggtatccaaa 780atagtctacg gccataccac cctgaacgtg
cctaatctcg taagctaagc agggtcaggc 840ctggttagta cctggatggg
gagagtatgg aaaacatacc tgcccgcagt tggagttgga 900ctctgtctta
acagtagcgt ggcacacaga aggcactcag taaatacttg ttgaataaat
960gaagtagcga tttggtgtga aaaaaaaaaa 990941638DNAHomo
sapiensmisc_featureIncyte ID No 1752821 94tagatatggc gtcctctttg
cttgcgggcg agcgattggt gcgtgctttg ggccccggcg 60gggagctgga gccagagcgg
ctaccccgaa agctgcgggc cgagcttgag gccgcgctgg 120ggaagaagca
caagggcggt gatagctcca gtggccccca acgcttggtt tctttccgtc
180tcatccggga tctgcaccag catctgagag aaagggattc caaactatac
ctccatgagc 240tcctagaagg cagtgaaatc tatctcccag aggttgtgaa
gcctccacgg aacccagaac 300tagttgcccg gctggagaag attaagatac
agctggccaa tgaggaatat aaacggatca 360cccgcaacgt cacttgtcag
gatacaagac atggtgggac tctcagcgac ctgggaaagc 420aagtgagatc
attgaaggct ctggtcatca ccatcttcaa tttcattgtc acggtggttg
480ctgccttcgt ctgcacttac cttggaagcc aatatatctt cacagaaatg
gcctcgcggg 540tgctagctgc attgatcgtc gcctctgtgg tgggtctggc
cgagctgtat gtcatggtgc 600gggcaatgga aggcgagctg ggagaactgt
aactggtgct tcatcatcaa gtctagagaa 660gactttgggg gcttcaggct
ccaattggca gtcaccgact cagtcaaccc atcagacttt 720ttgtattcag
ctccagttag tcagaagacc agcccaggcc agctgctgtt tctgtgggga
780gccctaatct tctgtgaatt tccaaaggga gcattggagg agattgagat
aacacatctt 840taaaacagaa agaactggtc ttggtctatc agtacctctt
cctgaatctg gtacccatct 900gccttctcca gttcattcta aacactgctg
ggactagggt ttttccatca ggagcaaatg 960gaatccaggc cttcccagaa
gtagaccata ctgccttgaa cttgtccata tgtacaaact 1020aatcaccagc
tttctccata catttttaat gcagacctgt aattgagttc agaagcctcc
1080aagaaaacag aaaggatccc ctttctccag tttgtgctgg aagaggagct
gatcagagac 1140atcaaataag agaaagatgg gttgctagag gatggtagaa
ctggaagcaa ggcagctacc 1200tttttgcaaa aggaaatggt gttaggcccc
ttttccagaa gataagacag actcatagag 1260attaaatgat cactatggtc
cttcttctgt taaatggagc caaagacgcc tatgttgttc 1320tgaagtcttg
taatgtttaa cttctgagaa cttagattag tggtgtgatg atagagtctg
1380tataacgcat tgaaaagggt atcaggctta gttatttatc caataaatat
ttattgtatg 1440cagggtattc ctattttaac tcctgtgaca acacaaagca
tagcgatttc catagttcta 1500actgttcagg gtctgctcct cctggtacac
tctttttggt tcactgtatg tactcctgtt 1560gtcttttttt ttttttccaa
agcacttttc tgttttcata aattatatac tcattcactc 1620agtggacaaa aaaaaaaa
163895595DNAHomo sapiensmisc_featureIncyte ID No 1810923
95gtgggcgcgt ccagtgatga ctgggggatc ccggcaagta acatgactaa aaagaagcgg
60gagaatctgg gcgtcgctct agagatcgat gggctagagg agaagctgtc ccagtgtcgg
120agagacctgg aggccgtgaa ctccagactc cacagccggg agctgagccc
agaggccagg 180aggtccctgg agaaggagaa aaacagccta atgaacaaag
cctccaacta cgagaaggaa 240ctgaagtttc ttcggcaaga gaaccggaag
aacatgctgc tctctgtggc catctttatc 300ctcctgacgc tcgtctatgc
ctactggacc atgtgagcct ggcacttccc cacaaccagc 360acaggcttcc
acttggcccc ttgatcagga tcaagcaggc acttcaagcc tcaataggac
420caaggtgctg gggtgttccc ctcccaacct agtgttcaag catggcttcc
tggcggccca 480ggccttgcct ccctggcctg ctggggggtt ccgggtctcc
agaaggacat ggtgctggtc 540cctcccttag cccaagggag aggcaataaa
gaacacaaag ctgtaaaaaa aaaaa 595961858DNAHomo
sapiensmisc_featureIncyte ID No 1822315 96aaaagtctag atcacagtgg
ggctctagga gggctagtcg ttggatttat cctaaccatt 60gcaaatttca gcttttttac
ctctttgctg atgtttttct tgtcttcttc gaaactcact 120aaatggaagg
gagaagtgaa gaagcgtcta gattcagaat ataaggaagg tgggcaaagg
180aattgggttc aggtgttctg
taatggagct gtacccacag aactggccct gctgtacatg 240atagaaaatg
gccccgggga aatcccagtc gatttttcca agcagtactc cgcttcctgg
300atgtgtttgt ctctcttggc tgcactggcc tgctctgctg gagacacatg
ggcttcagaa 360gttggcccag ttctgagtaa aagttctcca agactgataa
caacctggga gaaagttcca 420gttggtacca atggaggagt tacagtggtg
ggccttgtct ccagtctcct tggtggtacc 480tttgtgggca ttgcatactt
cctcacacag ctgatttttg tgaatgattt agacatttct 540gccccgcagt
ggccaattat tgcatttggt ggtttagctg gattactagg atcaattgtg
600gactcatact taggggctac aatgcagtat actgggttgg atgaaagcac
tggcatggtg 660gtcaacagcc caacaaataa ggcaaggcac atagcaggga
aacccattct tgataacaac 720gcgtggatct gttttcttct gttcttattg
ccctcttgct cccaactgct gcttggggtt 780tttggcccag ggggtgaact
ttatttcatt tccacaggtt gaaactggtg agtccagcta 840aatttgcaat
tccaactttc atcctaagaa taataactgt aatggcaaag cggaaatgcc
900agttcctcct gtattccatt gagatgggat ttcacatttt cctctcatca
actcccctgt 960aatagctagc gtctttctag tgaaagagaa gaattcctag
aacttatgca tttttttcct 1020gctgaatgga agtcttgagc aatgaagcta
tattgtccct acatattact atatattgaa 1080ctgaaagttc ttacataatc
aatgtcaagt tttgtcttat tttgttttgt ttgtttaaac 1140cagtgtagga
aataaaagtg atgatattta aaatagttct cagttgaagc agagaaatgc
1200cactgtgcta gttgcccaaa tgttgtatct attttaaata gtttaagctg
atgtgtatgg 1260gagcctaaac aagtgtagta tcctgaactt ctcccattaa
ttgctattca caattgggaa 1320aagtgtggag attggttcct agtgagtttt
gtggcctact ccacatttgt tcttccttcc 1380tcagggttag tgatgaaaaa
aagtaaatat ctttttcata tgtccattag aatgtatgaa 1440aaaaatcatt
ttaactaaaa gcaaaagaat tttatcttat atctaaaaaa tatataactt
1500actatatgtt tcagttgctc tctgaacaaa aattatcttc aatttaatat
gtggaatgtg 1560ttttctagct ttctttgaat tatgtatggc aacctggttt
agcactggca tcctgaacag 1620ttaagagtca ctgggaaatt attgtatttc
tttataaatt tactgtcata tcaattgctg 1680gaaaatgcta tgatttttct
attattacct tctaagttgt attctctctt acactgtagc 1740ctcaactaag
gcaattctgc tatgtttgtt cttcactatg atttactgtg tgccaaagga
1800gttttgacag ggtacagagt attttactaa aagtattttt aaatgttaaa aaaaaaaa
185897698DNAHomo sapiensmisc_featureIncyte ID No 1877777
97tgggtgtccg catgacaacc gacgttggag tttggaggtg cttgccttag agcaagggaa
60acagctctca ttcaaaggaa ctagaagcct ctccctcagt ggtagggaga cagccaggag
120cggttttctg ggaactgtgg gatgtgccct tgggggcccg agaaaacaga
aggaagatgc 180tccagaccag taactacagc ctggtgctct ctctgcagtt
cctgctgctg tcctatgacc 240tctttgtcaa ttccttctca gaactgctcc
aaaagactcc tgtcatccag cttgtgctct 300tcatcatcca ggatattgca
gtcctcttca acatcatcat cattttcctc atgttcttca 360acaccttcgt
cttccaggct ggcctggtca acctcctatt ccataagttc aaagggacca
420tcatcctgac agctgtgtac tttgccctca gcatctccct tcatgtctgg
gtcatgaact 480tacgctggaa aaactccaac agcttcatat ggacagatgg
acttcaaatg ctgtttgtat 540tccagagact agcagcagtg ttgtactgct
acttctataa acggacagcc gtaagactag 600gcgatcctca cttctaccag
gactctttgt ggctgcgcaa ggagttcatg caagttcgaa 660ggtgacctct
tgtcacactg atggatactt ttccttcc 698981476DNAHomo
sapiensmisc_featureIncyte ID No 1879819 98caaggacgag gctctggcca
agctgggtat caacggtgcc cactcgtccc cgccgatgct 60gtcccccagc ccaggaaagg
gccccccgcc agctgtggct cctcgaccca aggccccgct 120acagcttggg
ccctctagct ccatcaagga aaagcagggg ccccttctgg acctgtttgg
180ccagaagctg cctattgccc acacaccccc acctccacca gcgccaccac
tgcctctgcc 240cgaggaccca gggacccttt cagcagagcg tcgttgcttg
acacagcccg tggaggacca 300gggggtctcc acccagctac tcgcgccctc
tggcagcgtg tgcttctcct acaccggcac 360gccctggaag ttgttcctac
gcaaggaggt gttctaccca cgggagaact tcagccatcc 420ctactacctg
aggctcctct gtgagcagat cctacgggac accttctccg agtcctgtat
480ccggatttcc cagaatgagc ggcggaaaat gaaagacctg ctgggaggct
tggaggtgga 540cctggattct ctcaccacca ccgaagacag cgtcaagaag
cgcatcgtgg tggccgctcg 600ggacaactgg gccaattact tctcccgctt
ctttcctgtc tcgggcgaga gtggcagcga 660cgtgcagctg ttagccgtgt
cccaccgtgg gctgcgactg ctcaaggtga cccaaggccc 720cggcctccgc
cccgaccagc tgaagattct ctgctcatac agctttgcgg aggtgctggg
780tgtggagtgc cggggcggct ccaccctgga gctgtcactg aagagcgagc
agctggtgct 840gcacacagcc cgggcaaggg ccatcgaggc gctggttgag
ctattcctga atgagcttaa 900gaaggactcc ggctatgtca tcgccctgcg
cagctacatc actgacaact gcagcctcct 960cagcttccac cgtggggacc
tcatcaagct gctgccggtg tgccaccctg gagccaggct 1020ggcagtttgg
ctctgccggg ggccgttccg gactctttcc tgccgacata gtgcagccgg
1080ctgccgctcc cgacttttcc ttctccaagg agcagaggag tggctggcac
aagggtcagc 1140tgtccaacgg ggaaccaggg ctggctcggt gggacagggc
ctcagaggtg aggaagatgg 1200gagagggaca agcagaggca aggcctgcct
gagactgagg aaggaaaggg gtttgaccac 1260tcccgaggct gccatgcggt
gggaccaccc tgctgtccgt ctcctgtggc tgcccctctg 1320cccgctcctg
atggctcgcc ttgtctctcc agcaagactg tgcactcctt gcaggcaggg
1380gctgggctgg atgctgctct tgtgtcccac gtggtactta gttcaaggct
gccccagcag 1440atgcttaata aacagctctt cactttaaaa aaaaaa
147699646DNAHomo sapiensmisc_featureIncyte ID No 1932945
99ccggctggag gtgacgctga ggcggcgagg gtgagtcggc gccggccgct accgcacttc
60gggcgctcgt ccctcatttc tctgtggtga atggcgacgg gatggagcgc gaggggagcg
120gcggcagcgg cgggtcggcc gggctcctgc agcagatcct gagcctgaag
gttgtgccgc 180gggtgggcaa cgggaccctg tgccccaact ctacttccct
ctgctccttc ccagagatgt 240ggtatggtgt attcctgtgg gcactggtgt
cttctctctt ctttcatgtc cctgctggat 300tactggccct cttcaccctc
agacatcaca aatatggtag gttcatgtct gtaagcatcc 360tgttgatggg
catcgtggga ccaattactg ctggaatctt gacaagtgca gctattgctg
420gagtttaccg agcagcaggg aaggaaatga taccatttga agccctcaca
ctgggcactg 480gacagacatt ttgcgtcttg gtggtctcct ttttacggat
tttagctact ctatagcata 540catccttatg ctgagatgtt gaacttaaac
tttatggaat cctccaaaag aatacattat 600ggagtgtagt gttttcttag
ttcttccaaa gggagccact tggatg 6461001735DNAHomo
sapiensmisc_featureIncyte ID No 2061026 100gccggctgcg ccatggcgtt
ggcgttggcg gcgctggcgg cggtcgagcc ggcctgcggc 60agccggtacc agcagttgca
gaatgaagaa gagtctggag aacctgaaca ggctgcaggt 120gatgctcctc
caccttacag cagcatttct gcagagagcg cagcatattt tgactacaag
180gatgagtctg ggtttccaaa gcccccatct tacaatgtag ctacaacact
gcccagttat 240gatgaagcgg agaggaccaa ggctgaagct actatccctt
tggttcctgg gagagatgag 300gattttgtgg gtcgggatga ttttgatgat
gctgaccagc tgaggatagg aaatgatggg 360attttcatgt taactttttt
catggcattc ctctttaact ggattgggtt tttcctgtct 420ttttgcctga
ccacttcagc tgcaggaagg tatggggcca tttcaggatt tggtctctct
480ctaattaaat ggatcctgat tgtcaggttt tccacctatt tccctggata
ttttgatggt 540cagtactggc tctggtgggt gttccttgtt ttaggctttc
tcctgtttct cagaggattt 600atcaattatg caaaagttcg gaagatgcca
gaaactttct caaatctccc caggaccaga 660gttctcttta tttattaaag
atgttttctg gcaaaggcct tcctgcattt atgaattctc 720tctcaagaag
caagagaaca cctgcaggaa gtgaatcaag atgcagaaca cagaggaata
780atcacctgct ttaaaaaaat aaagtactgt tgaaaagatc atttctctct
atttgttcct 840aggtgtaaaa ttttaatagt taatgcagaa ttctgtaatc
attgaatcat tagtggttaa 900tgtttgaaaa agctcttgca atcaagtctg
tgatgtatta ataatgcctt atatattgtt 960tgtagtcatt ttaagtagca
tgagccatgt ccctgtagtc ggtagggggc agtcttgctt 1020tattcatcct
ccatctcaaa atgaacttgg aattaaatat tgtaagatat gtataatgct
1080ggccatttta aaggggtttt ctcaaaagtt aaacttttgt tatgactgtg
tttttgcaca 1140taatccatat ttgctgttca agttaatcta gaaatttatt
caattctgta tgaacacctg 1200gaagcaaaat catagtgcaa aaatacattt
aaggtgtggt caaaaataag tctttaattg 1260gtaaataata agcattaatt
ttttatagcc tgtattcaca attctgcggt accttattgt 1320acctaaggga
ttctaaaggt gttgtcactg tataaaacag aaagcactag gatacaaatg
1380aagcttaatt actaaaatgt aattcttgac actctttcta taattagcgt
tcttcacccc 1440cacccccacc cccacccccc ttattttcct tttgtctcct
ggtgattagg ccaaagtctg 1500ggagtaagga gaggattagg tacttaggag
caaagaaaga agtagcttgg aacttttgag 1560atgatcccta acatactgta
ctacttgctt ttacaatgtg ttagcagaaa ccagtgggtt 1620ataatgtaga
atgatgtgct ttctgcccaa gtggtaattc atcttggttt gctatgttaa
1680aactgtaaat acaacagaac attaataaat atctcttgtg tagcaaaaaa aaaaa
17351012329DNAHomo sapiensmisc_featureIncyte ID No 2096687
101gcagggatca ctagcatgtc tgcggagagc ggccctggga cgagattgag
aaatctgcca 60gtaatggggg atggactaga aacttcccaa atgtctacaa cacaggccca
ggcccaaccc 120cagccagcca acgcagccag caccaacccc ccgcccccag
agacctccaa ccctaacaag 180cccaagaggc agaccaacca actgcaatac
ctgctcagag tggtgctcaa gacactatgg 240aaacaccagt ttgcatggcc
tttccagcag cctgtggatg ccgtcaagct gaacctccct 300gattactata
agatcattaa aacgcctatg gatatgggaa caataaagaa gcgcttggaa
360aacaactatt actggaatgc tcaggaatgt atccaggact tcaacactat
gtttacaaat 420tgttacatct acaacaagcc tggagatgac atagtcttaa
tggcagaagc tctggaaaag 480ctcttcttgc aaaaaataaa tgagctaccc
acagaagaaa ccgagatcat gatagtccag 540gcaaaaggaa gaggacgtgg
gaggaaagaa acagggacag caaaacctgg cgtttccacg 600gtaccaaaca
caactcaagc atcgactcct ccgcagaccc agacccctca gccgaatcct
660cctcctgtgc aggccacgcc tcaccccttc cctgccgtca ccccggacct
catcgtccag 720acccctgtca tgacagtggt gcctccccag ccactgcaga
cgcccccgcc agtgcccccc 780cagccacaac ccccacccgc tccagctccc
cagcccgtac agagccaccc acccatcatc 840gcggccaccc cacagcctgt
gaagacaaag aagggagtga agaggaaagc agacaccacc 900acccccacca
ccattgaccc cattcacgag ccaccctcgc tgcccccgga gcccaagacc
960accaagctgg gccagcggcg ggagagcagc cggcctgtga aacctccaaa
gaaggacgtg 1020cccgactctc agcagcaccc agcaccagag aagagcagca
aggtctcgga gcagctcaag 1080tgctgcagcg gcatcctcaa ggagatgttt
gccaagaagc acgccgccta cgcctggccc 1140ttctacaagc ctgtggacgt
ggaggcactg ggcctacacg actactgtga catcatcaag 1200caccccatgg
acatgagcac aatcaagtct aaactggagg cccgtgagta ccgtgatgct
1260caggagtttg gtgctgacgt ccgattgatg ttctccaact gctataagta
caaccctcct 1320gaccatgagg tggtggccat ggcccgcaag ctccaggatg
tgttcgaaat gcgctttgcc 1380aagatgccgg acgagcctga ggagccagtg
gtggccgtgt cctccccggc agtgccccct 1440cccaccaagg ttgtggcccc
gccctcatcc agcgacagca gcagcgatag ctcctcggac 1500agtgacagtt
cgactgatga ctctgaggag gagcgagccc agcggctggc tgagctccag
1560gagcagctca aagccgtgca cgagcagctt gcagccctct ctcagcccca
gcagaacaaa 1620ccaaagaaaa aggagaaaga caagaaggaa aagaaaaaag
aaaagcacaa aaggaaagag 1680gaagtggaag agaataaaaa aagcaaagcc
aaggaacctc ctcctaaaaa gacgaagaaa 1740aataatagca gcaacagcaa
tgtgagcaag aaggagccag cgcccatgaa gagcaagccc 1800cctcccacgt
atgagtcgga ggaagaggac aagtgcaagc ctatgtccta tgaggagaag
1860cggcagctca gcttggacat caacaagctc cccggcgaga agctgggccg
cgtggtgcac 1920atcatccagt cacgggagcc ctccctgaag aattccaacc
ccgacgagat tgaaatcgac 1980tttgagaccc tgaagccgtc cacactgcgt
gagcttggag cgctatgtca cctcctgttt 2040gcggaagaaa aggaaacctt
caagctgaga aagttgatgt gatntgccgg gttcctccaa 2100natgaaaggn
ttctcggtct tcaagagncg ggagagnctc ccagttgaat tccaanttct
2160tttgacaagc ggaaganttc cggaaaacaa agggtccttg gccttaaatt
caatttggga 2220aaaccnggga cttccttaaa tttaaaaaaa gggggctttt
caagntttcc caaggaattt 2280ccttttcccc caaggnaaag gcntaattan
gcctttaaaa ggttnccca 23291021451DNAHomo sapiensmisc_featureIncyte
ID No 2100530 102ctcgagcggc ggcatttcct ggtgtctgag cctggcgcgg
aggctatggg cagccaggag 60gtgctgggcc acgcggcccg gctggcctcc tccggtctcc
tcctgcaggt gttgtttcgg 120ttgatcacct ttgtcttgaa tgcatttatt
cttcgcttcc tgtcaaagga aatcgttggc 180gtagtaaatg taagactaac
gctgctttac tcaaccaccc tcttcctggc cagagaggcc 240ttccgcagag
catgtctcag tgggggcacc cagcgagact ggagccagac cctcaacctg
300ctgtggctaa cagtccccct gggtgtgttt tggtccttat tcctgggctg
gatctggttg 360cagctgcttg aagtgcctga tcctaatgtt gtccctcact
atgcaactgg agtggtgctg 420tttggtctct cggcagtggt ggagcttcta
ggagagccct tttgggtctt ggcacaagca 480catatgtttg tgaagctcaa
ggtgattgca gagagcctgt cggtaattct taagagcgtt 540ctgacagctt
ttctcgtgct gtggttgcct cactggggat tgtacatttt ctctttggcc
600cagcttttct ataccacagt tctggtgctc tgctatgtta tttatttcac
aaagttactg 660ggttccccag aatcaaccaa gcttcaaact cttcctgtct
ccagaataac agatctgtta 720cccaatatta caagaaatgg agcgtttata
aactggaaag aggctaaact gacttggagt 780tttttcaaac agtctttctt
gaaacagatt ttgacagaag gcgagcgata tgtgatgaca 840tttttgaatg
tattgaactt tggtgatcag ggtgtgtatg atatagtgaa taatcttggc
900tcccttgtgg ccagattaat tttccagcca atagaggaaa gtttttatat
attttttgct 960aaggtgctgg agaggggaaa ggatgccaca cttcagaagc
aggaggacgt tgctgtggct 1020gctgcagtct tggagtccct gctcaagctg
gccctgctgg ccggcctgac catcactgtt 1080tttggctttg cctattctca
gctggctctg gatatctacg gagggaccat gcttagctca 1140ggatccggtc
ctgttttgct gcgttcctac tgtctctatg ttctcctgct tgccatcaat
1200ggagtgacag agtgtttcac atttgctgcc atgagcaaag aggaggtcga
caggtattcc 1260tctgctgtga gcagggctgg ccagccagac tggcacacat
tgctgtgggg gccttctgtc 1320tgggagcaac tctcgggaca gcattnctca
cagagaccaa gctgatccat ttnctcagga 1380ctcagttagg tgtgcccaga
cggactgaca aaatgacgtg acttcagggn aggctgggac 1440aaacgaggca a
14511031685DNAHomo sapiensmisc_featureIncyte ID No 2357636
103gcgatcgagg ctgcagcgcg gccgccgggc gcacatgact gccgtcggcg
tgcaggccca 60gaggcctttg ggccaaaggc agccccgccg gtccttcttt gaatccttca
tccggaccct 120catcatcacg tgtgtggccc tggctgtggt cctgtcctcg
gtctccattt gtgatgggca 180ctggctcctg gctgaggacc gcctcttcgg
gctctggcac ttctgcacca ccaccaacca 240gagtgtgccg atctgcttca
gagacctggg ccaggcccat gtgcccgggc tggccgtggg 300catgggcctg
gtacgcagcg tgggcgcctt ggccgtggtg gccgccattt ttggcctgga
360gttcctcatg gtgtcccagt tgtgcgagga caaacactca cagtgcaagt
gggtcatggg 420ttccatcctc ctcctggtgt ctttcgtcct ctcctccggc
gggctcctgg gttttgtgat 480cctcctcagg aaccaagtca cactcatcgg
cttcacccta atgttttggt gcgaattcac 540tgcctccttc ctcctcttcc
tgaacgccat cagcggcctt cacatcaaca gcatcaccca 600tccctgggaa
tgaccgtgga aattttaggc cccctccagg gacatcagat tccacaagaa
660aatatggtca aaatgggact tttccagcat gtggcctctg gtggggctgg
gttggacaag 720ggccttgaaa cggctgcctg tttgccgata acttgtgggt
ggtcagccag aaatggccgg 780ggggcctctg cacctggtct gcagggccag
aggccaggag ggtgcctcag tgccaccaac 840tgcacaggct tagccagatg
ttgattttag aggaagaaaa aaacatttta aaactccttc 900ttgaattttc
ttccctggac tggaatacag ttggaagcac aggggtaact ggtacctgag
960ctagctgcac agccaaggat agttcatgcc tgtttcattg acacgtgctg
ggataggggc 1020tgcagaatcc ctggggctcc cagggttgtt aagaatggat
cattcttcca gctaagggtc 1080caatcagtgc ctattcttcc accagctcaa
agggccttcg tatgtatgtc cctggcttca 1140gctttggtca tgccaaagag
gcagagttca ggattccctc agaatgccct gcacacagta 1200ggtttccaaa
ccatttgact cggtttgcct ccctgcccgt tgtttaaacc ttacaaaccc
1260tggataaccc catcttctag cagctggctg tcccctctgg gagctctgcc
tatcagaacc 1320ctaccttaag gtgggtttcc ttccgagaag agttcttgag
caagctctcc caggagggcc 1380cacctgactg ctaatacaca gccctcccca
aggcccgtgt gtgcatgtgt ctgtcttttg 1440tgagggttag acagcctcag
ggcaccattt ttaatcccag aacacatttc aaagagcacg 1500tatctagacc
tgctggactc tgcagggggt gagggggaac agcgagagct tgggtaatga
1560ttaacaccca tgctggggat gcatggaggt gaagggggcc aggaaccagt
ggagatttcc 1620atccttgcca gcacgtctgt acttctgttc attaaagtgc
tccctttcta gtcaaaaaaa 1680aaaaa 16851042674DNAHomo
sapiensmisc_featureIncyte ID No 2365230 104ctactcctca ccgcgcgagc
gcggggaacc agtagccgcg gctgcttcgg ttgccgcggt 60cggtggtcgt tatggattct
ccatgggacg agttggctct ggccttctcc cgcacgtcca 120tgtttccctt
ttttgacatc gcgcactatc tagtgtcagt gatggcggtg aaacgtcagc
180cgggagcagc tgcattggca tggaagaatc ctatttcaag ctggtttact
gctatgctcc 240actgttttgg tggaggaatt ttatcctgtc tactgcttgc
agagcctcca ttgaagtttc 300ttgcaaacca cactaacata ttactggcat
cttcaatctg ggtatattac atttttttgc 360ccgcatgacc tagtttccca
gggctattca tatctacctg ttcaactact ggcttcggga 420atgaaggaag
tgaccagaac ttggaaaata gtaggtggag tcacacatgc taatagctat
480tacaaaaatg gctggatagt catgatagct attggatggg cccgaggtgc
aggtggtacc 540attataacga attttgagag gttggtaaaa ggagattgga
aaccagaagg tgatgaatgg 600ctgaagatgt cataccctgc caaggtaacc
ctgctggggt cagttatctt cacattccag 660cacacccagc atctggcaat
atcaaagcat aatcttatgt tcctttatac catctttatt 720gtggccacaa
agataaccat gatgactaca cagacttcta ctatgacatt tgctcctttt
780gaggatacat tgagttggat gctatttggc tggcagcagc cgttttcatc
atgtgagaag 840aaaagtgaag caaagtcacc ttccaatggc gttgggtcat
tggcctcaaa gccggtagat 900gttgcctcag ataatgttaa aaagaaacat
actaagaaga atgaataaat ttacgtgatg 960agctctagca agccaaaaat
tttttttctt atctacctgt tatattgtgc taattttcta 1020tgtatgtgat
gtgaaatgaa gactatatat atggaatgga ggtgacagaa agaaagaaat
1080tctttgtttg agggagactt cccctttctg gattgtattt gtagagtgtt
acgagtgtat 1140catgtgatta tgctttaccg gtataagaga ttctgttgtg
attatttgaa tagttttata 1200ttaataaaag aagacaaaat tttttaaatg
ttagaaaaag cagatctgtc attgcaaagt 1260aacaaaaatt ttaagctttt
aaaaatgtag atttttcata tttttaaaat ttgaatctat 1320ttgagcttta
gttcagcaga attaaatttt tacttgacat tatcattaaa attgctaggt
1380atggagaaca attcctattt tattttgaac actgagaaga gtaaactttt
cctaaaacac 1440tttatattat aaatgaaaat aaattgctag tttatatttt
agatataaac atcatatttt 1500ttattaatac ctacatcaaa tggaaaatat
ctgaaatttt ttttccatag caggtatttt 1560ctactagaag tagttttact
acttttcatt tagaacagag tatgagtctt aatctgaagt 1620ctttttcatg
cccttgtttt aaaaaaacta ctttttttgg cctcaaaaaa atcaagggtg
1680taatttttaa taaattgtta atcctatgtt ttgtaatttt cattttagga
gcttgactta 1740ttttttttct ctctcataaa aacacatttg ttttaattgt
aggagaaatt ttctcagcat 1800tttgcatgtt ctttctaatc tttgttggtc
tgaatatatt ggtagtaatt actgtaatta 1860ttcaacaaaa agcatatccg
ttcaaaaatt tttccactat gtcttttttc tagtggctac 1920tgttttagtt
ttctagttga atatctctga caagctttcg tatggttttg ttatattttc
1980atctacatgt aatgtgttat taattttatt aaatgaaaac taatcacctt
catgtggaaa 2040tgctctgaga attgtcctta ggcatttggt agtaaccagc
taaccaagaa gaaacagaga 2100aaccagaact tcatatggca gtccatttag
atgaagaatg atgatataaa atctggttcc 2160ttcttagcaa aataaaaaac
aaacaagaaa agatactaaa tgatgttaat tttcttactt 2220tatgatttag
aagtccagtt ataatattaa aactctgtga catagtttct tttaccaaaa
2280ccatgaacct actccccgta tcaggtattt tcgatggttt agaagtactc
aagtcacatc 2340acattcaagt tagaagtttt ttttttgttg ttgttatttt
aaatttttaa caaatataaa 2400caccagcaga tactattact tgcttaaaaa
attgggaggg ggcacttttc atagtcttgg 2460aatgctaaga agttttattt
ttaatattgt gacagaaagc tttaagtatt taagagctct 2520gtattatatt
tgatactctt acagttaaaa acttttcaaa attaatacat tgttaattat
2580tgaccagttt tgaagtttgg gtttaactgt agttgaaatg gaaggactct
tgttttacac 2640ttgtattaaa gataaattta ttaaaataag ttat
2674105488DNAHomo
sapiensmisc_featureIncyte ID No 2455121 105gactacgggg ctgttgacgg
cgctgcgatg gctgcctgcg agggcaggag aagcggactc 60tcggttcctc tcagtcggac
ttcctgacgc cgccagttgg cggggcccct tgggccgtcg 120ccaccactgt
agtcatgtac ccaccgccgc cgccgccgcc tcatcgggac ttcatctcgg
180tgacgctgag ctttggcgag agctatgaca acagcaagag ttggcggcgg
cgctcgtgct 240ggaggaaatg gaagcaactg tcgagattgc agcggaatat
gattctcttc ctccttgcct 300ttctgctttt ctgtggactc ctcttctaca
tcaacttggc tgaccattgg aaagctctgg 360ctttcaggct aggggaagag
cagaagatga ggccagaaat tgctgggtaa aaccagcaaa 420tccacccgtc
ttaccagctc ctcagaaggc ggacaccggc cctgagaact tacctgagat 480ttcgtcac
4881061028DNAHomo sapiensmisc_featureIncyte ID No 2472514
106ccagcagctc ggtcctaggg cgatgttgac agacagacag aggggcggat
gcagcctacc 60tcctgggcag tgagctgcgg tctgaggccc ctgcccagct ggaaaccaca
gggaggggaa 120gggaggggag gagaggagag gagaggaacc gtcatggggc
cttggagtcg agtcagggtt 180gccaaatgcc agatgctggt cacctgcttc
tttatcttgc tgctgggcct ctctgtggcc 240accatggtga ctcttaccta
cttcggggcc cactttgctg tcatccgccg agcgtccctg 300gagaagaacc
cgtaccaggc tgtgcaccaa tgggccttct ctgcggggtt gagcctggtg
360ggcctcctga ctctgggagc cgtgctgagc gctgcagcca ccgtgaggga
ggcccagggc 420ctcatggcag ggggcttcct gtgcttctcc ctggcgttct
gcgcacaggt gcaggtggtg 480ttctggagac tccacagccc cacccaggtg
gaggacgcca tgctggacac ctacgacctg 540gtatatgagc aggcgatgaa
aggtacgtcc cacgtccggc ggcaggagct ggcggccatc 600caggacgtgg
tgagcgtggg gacggctggg tggcagggcg gtcagcttct gcttggactg
660cagttcagag aacaggcgca gggtggccag tgagaggtct ggccaggcac
cgagggggtt 720ccaggacaca ggccagagtt gcccctcagg gctgggggca
aaaagctccc accctctgtc 780tgcccaggac aaggccgcct accagattct
cgaggcccag tgcaaaacga gagggcaggg 840ccctgtattc agaaacactg
aaggatttca agagcattaa agcaaatacg gggccgaaca 900tagtggctca
cacctgtaat cccagcactt tgggaggagg ttgaggcagg tgaattgctt
960gagcccagga gttcgagacc agcctgagca acatagggag accttgtctc
tactttaaaa 1020aaaaaaaa 10281071551DNAHomo
sapiensmisc_featureIncyte ID No 2543486 107ctgcgcctgg gctgccggtg
acctgggccg agccctcccg gtcggctaag attgctgagg 60aggcggcggg tagctggcag
gcgccgactt ccgaaggccg ccgtccgggc gaggtgtcct 120catgacttct
cttgtggacc atgtccgtga tcttttttgc ctgcgtggta cgggtaaggg
180atggactgcc cctctcagcc tctactgatt tttaccacac ccaagatttt
ttggaatgga 240ggagacggct caagagttta gccttgcgac tggcccagta
tccaggtcga ggttctgcag 300aaggttgtga ctttagtata catttttctt
ctttcgggga cgtggcctgc atggctatct 360gctcctgcca gtgtccagca
gccatggcct tctgcttcct ggagaccctg tggtgggaat 420tcacagcttc
ctatgacact acctgcattg gcctagcctc caggccatac gcttttcttg
480agtttgacag catcattcag aaagtgaagt ggcattttaa ctatgtaagt
tcctctcaga 540tggagtgcag cttggaaaaa attcaggagg agctcaagtt
gcagcctcca gcggttctca 600ctctggagga cacagatgtg gcaaatgggg
tgatgaatgg tcacacaccg atgcacttgg 660agcctgctcc taatttccga
atggaaccag tgacagccct gggtatcctc tccctcattc 720tcaacatcat
gtgtgctgcc ctgaatctca ttcgaggagt tcaccttgca gaacattctt
780tacaggttgc ccatgaggaa attggaaaca ttctggcttt tcttgttcct
ttcgtagcct 840gcattttcca ggatccaagg agctggttct gctggttgga
ccaaacctcg tgagccagcc 900acccctgacc caaatgagga gagctctgat
tctcccatcc gggagcagtg atgtcaaact 960tctgctgctg gggaaatctc
atcagcaggg agcctgtgga aaagggcatg tcagtgaaat 1020ctgggaatgg
ctggattcgg aaacatctgc ccatgtgtat tgatggcaga gctgttgccc
1080acaagcgcct tttatttagg gtaaaattaa caaatccatt ctattcctct
gacccatgct 1140tagtacatat gacctttaac ccttacattt atatgattct
ggggttgctt cagaagtgtt 1200atttcatgaa tcattcatat gatttgatcc
cccaggattc tattttgttt aatgggcttt 1260tctactaaaa gcataaaata
ctgaggctga tttagtcagg gcaaaaccat ttactttaca 1320tattcgtttt
caatacttgc tgttcatgtt acacaagctt cttacggttt tcttgtaaca
1380ataaatattt tgagtaaata atgggtacat tttaacaaac tcagtagtac
aacctaaact 1440tgtataaaag tgtgtaaaaa tgtatagcca tttatatcct
atgtataaat taaatgaggt 1500ggcttcagaa atggcagaat aaatctaaag
tgtttattaa caaaaaaaaa a 1551108922DNAHomo sapiensmisc_featureIncyte
ID No 2778171 108gcttgcggct cgggtggctg agcgcgcggg gaaatggcca
cggggacaga ccaggtggtg 60ggactcggcc tcgtcgccgt tagcctgatc atcttcacct
actacaccgc ctgggtgatt 120ctcttgccat tcatcgacag tcagcatgtc
atccacaagt atttcctgcc ccgagcctat 180gctgtcgcca tcccactggc
tgcaggcctc ctgctgctcc tgtttgtggg actgttcatc 240tcctacgtga
tgctgaagag caagagagtg accaagaagg ctcagtgaag gtcccgcagg
300atgaggctgc cagccccttc tctgcttccc ctccagcaca gggaccaagt
gggggagcct 360gcagaacctg tccaggcaca gtggctcctc aagcctgcct
gtcctgcaga gtccccatgg 420catggagctt acacctgact gactggagcc
ccctccccga ctcccacttc cagaagctag 480gagggaggga tacctggaag
actccggtca cctccttctt gctcagggcc taaaagatgc 540tggtcctccc
aacctcactc tcagactccc tgccaccttt tcccctgggt tctgccgtct
600tgcctcactt cccctcctgt cacatgctga cgttggactt agcaggttct
aaggccacat 660gtgtgacctc tctgacttct cttcctccac caaggcagct
ttccttaccc tgacacagcc 720ccagacccca caaagccttc tggacctgga
aagcctgggg aaggactgac agaccccagg 780accagccctg gggctcaggg
cagccacccc gggccgctga ccgactgacc tctcctcacg 840gaggcccagc
cccaaagccc cagggctggc ccgtttggga cagctgacca ataaacactg
900atggtgtgtt aaaaaaaaaa aa 922109985DNAHomo
sapiensmisc_featureIncyte ID No 2799575 109gcccaggagg cgcccgggtg
aggcacgggt gcgcaagcga ggagttccgg ctggagaccc 60gtgctctggg ccggcgcctt
caccatggcc tcggcagagc tggactacac catcgagatc 120ccggatcagc
cctgctggag ccagaagaac agccccagcc caggtgggaa ggaggcagaa
180actcggcagc ctgtggtgat tctcttgggc tggggtggct gcaaggacaa
gaaccttgcc 240aagtacagtg ccatctacca caaaaggggc tgcatcgtaa
tccgatacac agccccgtgg 300cacatggtct tcttctccga gtcactgggt
atcccttcac ttcgtgtttt ggcccagaag 360ctgctcgagc tgctctttga
ttatgagatt gagaaggagc ccctgctctt ccatgtcttc 420agcaacggtg
gcgtcatgct gtaccgctac gtgctggagc tcctgcagac ccgtcgcttc
480tgccgcctgc gtgtggtggg caccatcttt gacagcgctc ctggtgacag
caacctggta 540ggggctctgc gggccctggc agccatcctg gagcgccggg
ccgccatgct gcgcctgttg 600ctgctggtgg cctttgccct ggtggtcgtc
ctgttccacg tcctgcttgc tcccatcaca 660gccctcttcc acacccactt
ctatgacagg ctacaggacg cgggctctcg ctggcccgag 720ctctacctct
actcgagggc tgacgaagta gtcctggcca gagacataga acgcatggtg
780gaggcacgcc tggcacgccg ggtcctggcg cgttctgtgg atttcgtgtc
atctgcacac 840gtcagccacc tccgtgacta ccctacttac tacacaagcc
tctgtgtcga cttcatgcgc 900aactgcgtcc gctgctgagg ccattgctcc
atctcacctc tgctccagaa ataaatgcct 960gacacctccc cacaaaaaaa aaaaa
9851101562DNAHomo sapiensmisc_featureIncyte ID No 2804955
110tgcgtccaga ggctggcatg gcgcgggccg agtactgagc gcacggtcgg
ggcacagcag 60ggccggtggg tgcagctggc tcgcgcctcc tctccggccg ccgtctcctc
cggtccccgg 120cgaaagcatt gagacaccag ctggacgtca cgcgccggag
catgtctggg agtcagagcg 180aggtggctcc atccccgcag agtccgcgga
gccccgagat gggacgggac ttgcggcccg 240ggtcccgcgt gctcctgctc
ctgcttctgc tcctgctggt gtacctgact cagccaggca 300atggcaacga
gggcagcgtc actggaagtt gttattgtgg taaaagaatt tcttccgact
360ccccgccatc ggttcagttc atgaatcgtc tccggaaaca cctgagagct
taccatcggt 420gtctatacta cacgaggttc cagctccttt cctggagcgt
gtgtggaggc aacaaggacc 480catgggttca ggaattgatg agctgtcttg
atctcaaaga atgtggacat gcttactcgg 540ggattgtggc ccaccagaag
catttacttc ctaccagccc cccaatttct caggcctcag 600agggggcatc
ttcagatatc cacacccctg cccagatgct cctgtccacc ttgcagtcca
660ctcagcgccc caccctccca gtaggatcac tgtcctcgga caaagagctc
actcgtccca 720atgaaaccac cattcacact gcgggccaca gtctggcagc
tgggcctgag gctggggaga 780accagaagca gccggaaaaa aatgctggtc
ccacagccag gacatcagcc acagtgccag 840tcctgtgcct cctggccatc
atcttcatcc tcaccgcagc cctttcctat gtgctgtgca 900agaggaggag
ggggcagtca ccgcagtcct ctccagatct gccggttcat tatatacctg
960tggcacctga ctctaatacc tgagccaaga atggaagttt gtgaggagac
ggactctatg 1020ttgcccaggc tgttatggaa ctcctgagtc aagtgatcct
cccaccttgg cctctgaagg 1080tgcgaggatt ataggcgtca cctaccacat
ccagcctaca cgtatttgtt aatatctaac 1140ataggactaa ccagccactg
ccctctctta ggcccctcat ttaaaaacgg ttatactata 1200aaatctgctt
ttcacactgg gtgataataa cttggacaaa ttctatgtgt attttgtttt
1260gttttgcttt gctttgtttt gagacggagt ctcgctctgt catccaggct
ggagtgcagt 1320ggcatgatct cggctcactg caacccccat ctcccaggtt
caagcgattc tcctgcctcc 1380tcctaagtag ctgggactac aggtgctcac
caccacaccc ggctaatttt ttgtattttt 1440agtagagacg gggtttcacc
atgttgacca ggctggtctc gaactcctga cctggtgatc 1500tgcccaccag
gcctcccaaa gtgctgggat taaaggtgtg agccacatgg ctggcctatg 1560tt
15621111851DNAHomo sapiensmisc_featureIncyte ID No 2806395
111gctctgcaga gtggtggccg gggccagggc cggggtgccc tccctcccac
cttctcccgc 60catgagccag ggaagtccgg gggactgggc ccccctagat cccacccccg
gacccccagc 120atcccccaac cccttcgtgc atgagttaca tctctctcgc
ctccagaggg ttaagttctg 180cctcctgggg gcattgctgg cccccatccg
agtgcttctg gcctttatcg tcctctttct 240cctctggccc tttgcctggc
ttcaagtggc cggtcttagt gaggagcagc ttcaggagcc 300aattacagga
tggaggaaga ctgtgtgcca caacggggtg ctaggcctga gccgcctgct
360gtttttcctg ctgggcttcc tccggattcg cgttcgtggc cagcgagcct
ctcgccttca 420agcccctgtc cttgttgctg ccccacactc cactttcttt
gaccccattg ttctgctgcc 480ctgtgacctg cccaaagttg tgtcccgagc
tgagaacctt tccgttcctg tcattggagc 540ccttcttcga ttcaaccaag
ccatcctggt atcccggcat gacccggctt ctcgacgcag 600agtggtggag
gaggtccgaa ggcgggccac ctcaggaggc aagtggccgc aggtgctatt
660ctttcctgag ggcacctgtt ccaacaagaa ggctttgctt aagttcaaac
caggagcctt 720catcgcaggg gtgcctgtgc agcctgtcct catccgctac
cccaacagtc tggacaccac 780cagctgggca tggaggggtc ctggagtact
caaagtcctc tggctcacag cctctcagcc 840ctgcagcatt gtggatgtgg
agttccttcc tgtgtatcac cccagccctg aggagagcag 900ggaccccacc
ctctatgcca acaatgttca gagggtcatg gcacaggctc tgggcattcc
960agccaccgaa tgtgagtttg tagggagctt acctgtgatt gtggtgggcc
ggctgaaggt 1020ggcgttggaa ccacagctct gggaactggg aaaagtgctt
cggaaggctg ggctgtccgc 1080tggctatgtg gacgctgggg cagagccagg
ccggagtcga atgatcagcc aggaagagtt 1140tgccaggcag ctacagctct
ctgatcctca gacggtggct ggtgcctttg gctacttcca 1200gcaggatacc
aagggtttgg tggacttccg agatgtggcc cttgcactag cagctctgga
1260tgggggcagg agcctggaag agctaactcg tctggccttt gagctctttg
ctgaagagca 1320agcagagggt cccaaccgcc tgctgtacaa agacggcttc
agcaccatcc tgcacctgct 1380gctgggttca ccccaccctg ctgccacagc
tttgcatgct gagctgtgcc aggcaggatc 1440cagccaaggc ctctccctct
gtcagttcca gaacttctcc ctccatgacc cactctatgg 1500gaaactcttc
agcacctacc tgcgcccccc acacacctct cgaggcacct cccagacacc
1560aaatgcctca tccccaggca accccactgc tctggccaat gggactgtgc
aagcacccaa 1620gcagaaggga gactgagtgc ctcagcctct caccccctcc
tcctcagggc agcgctaggg 1680gcctccccta tgcctcagcc ccatctctgc
tcctgtttga attttgttat tgttgtttgg 1740ttgttgtttt tttaagttga
ttttaatttt ttgtttggtt gatttttttg taaaaaacta 1800ttttatatat
aaatataaat ctatatctat atctattaaa aaaaatgaat t 1851112992DNAHomo
sapiensmisc_featureIncyte ID No 2836858 112ggcgcgaggc agtatggttt
gaagtggtga acatggattt ttctcggctt cacatgtaca 60gtcctcccca gtgtgtgccg
gagaacacgg gctacacgta tgcgctcagt tccagctatt 120cttcagatgc
tctggatttt gagacggagc acaaattgga ccctgtattt gattctccac
180ggatgtcccg ccgtagtttg cgcctggcca cgacagcatg caccctgggg
gatggtgagg 240ctgtgggtgc cgacagcggc accagcagcg ctgtctccct
gaagaaccga gcggccagaa 300caacaaaaca gcgcagaagc acaaacaaat
cagcttttag tatcaaccac gtgtcaaggc 360aggtcacgtc ctctggcgtc
agccacggcg gcactgtcag cctgcaggat gctgtgactc 420gacggcctcc
tgtattggac gagtcttgga ttcgtgaaca gaccacagtg gaccacttct
480ggggtcttga tgatgatggt gatcttaaag gtggaaataa agctgccatt
cagggaaacg 540gggatgtggg agccgccgcc gccaccgcgc acaacggctt
ctcctgcagc aactgcagca 600tgctgtccga gcgcaaggac gtgctcacgg
cgcaccccgc ggcccccggg cccgtgtcga 660gagtttattc tagggacagg
aatcaaaaat gtaagtctca gtcctttaaa actcagaaaa 720aggtgtgttt
tccaaattta atatttcctt tctgtaagtc tcagtgtctg cactatttgt
780cttggagact taaaattatc ccttgaaagc ataagaagta caccccaaac
cagctttgtc 840cttcctgtcc tcttctagtt tacattttat gtggttagta
attttgtacc taaaagtatt 900tgaaattcta taaatttgga cttgacgtga
gcaaaagaaa atttctacgt aagcgaaact 960aataaaacta cagtcacttt
caaaaaaaaa aa 9921131251DNAHomo sapiensmisc_featureIncyte ID No
2844513 113ctctgctggc cggtctaaag cggcagccgc cggggcgcaa tgcgagcggc
tggcgtaggc 60ttggtggact gtcactgcca cctctccgcc ccggactttg accgcgattt
ggatgatgtg 120ttggagaaag ccaagaaggc caatgttgtg gcccttgtgg
cagttgccga acattcagga 180gaatttgaaa agattatgca actttcagaa
aggtataatg ggtttgtcct gccatgcttg 240ggtgttcatc cagttcaagg
acttccacca gaagaccaaa gaagtgtcac actaaaggat 300ttggatgtag
ctttgcccat tattgagaat tataaggatc ggttgttggc aattggagag
360gttggactag atttctcccc cagatttgct ggcactggtg aacagaagga
agagcaaaga 420caagtcctaa tcagacagat ccagttagcc aaaagactaa
atttgcctgt aaatgtgcac 480tcacgctctg ctggaagacc taccatcaac
cttttacaag agcaaggtgc tgagaaggta 540ctgctgcatg catttgatgg
tcggccatct gtagccatgg aaggagtaag agctgggtac 600ttcttctcaa
ttcccccttc tatcataaga agtggacaga agcagaaact tgtgaaacaa
660ttgcctttaa cttctatatg cttagaaaca gattcacctg cactaggacc
agaaaaacag 720gtacggaatg agccctggaa catttctatt tcagcagaat
atattgccca ggtgaaaggg 780atctcagtgg aagaagttat agaagtgacg
acacagaatg cattaaaact gtttcctaag 840ctccgacact tgctccagaa
atagcttcaa aaccatccat tacaaaatcg aatcaactgc 900agggggcagc
atttgaaaaa tagaaatgtt ctgatgaaga atctgaactg aagaagctgt
960tttatagggt tatagaagat tgtaattgta gagaaatatt tctcttagaa
ataaaactgg 1020gcttggatcc tgaaaccctg ggttctgatt ctagccttgt
gctgcttttc aattagccga 1080gttctggcag gatattggga aaatactgct
acttcttaca ttgccctttt atatagaacc 1140accacctgaa ctgaaaccat
tgctactggg aagggtggct cccacaggaa gagtataagc 1200actactgtga
tgaggatgga gtaagctaaa gtatactttt tttttttttt g 12511141397DNAHomo
sapiensmisc_featureIncyte ID No 3000380 114ctaggacgcc cctggagccg
gaaccccagc agaagccgga accagaacca aatcaccggt 60accggctgca gccccctaaa
cccaggaggc gccctggccc gcgctcgccc cccagggcct 120catgtcggaa
ccacagcctg acctggaacc gccccaacat gggctatata tgctcttcct
180gcttgtgctg gtcttcttcc tcatgggcct ggtaggcttc atgatctgcc
acgtgctcaa 240gaagaagggc taccgctgcc gcacgtcgag gggctctgag
cctgacgatg cccagcttca 300gccccctgag gacgatgaca tgaatgagga
cacagtagag aggattgttc gctgcatcat 360ccagaatgaa gtgtggatgc
cacctccagc ctgcaggacg gagccccctc ccatcatcac 420acagtgcacc
tgggctctgc agccccttgc cgtccattgc agccgcagca agaggcctcc
480acttgtccgt cagggacgct ccaaggaagg aaaaagccgc ccccggacag
gggagaccac 540tgtgttctct gtgggcaggt tccgggtgac acacattgag
aagcgctatg gactgcacga 600acaccgtgat ggctccccca cagacaggag
ctggggctct cgtgggggac aggacccagg 660gggtggtcag gggtctgggg
gagggcaccc caaggcaggg atgctgccat ggagaggctg 720cccccctgag
aggccacagc cccaggtcct agccagcccc ccagtacaga atggaggact
780cagggacagc agcctaaccc ctcgtgcact tgaagggaac cccagagctt
ctgcagagcc 840aacactgagg gccggaggga ggggcccaag cccagggctg
cccactcaag aggcaaatgg 900gcagccaagc aaaccagaca cttctgatca
ccaggtgtct ctaccacagg gagcagggag 960tatgtgagtc tccttcattg
tgctgatgga ctaccagctg gcagggccag ggggtgggtg 1020ggcgtgaaag
ccctcccctc cactggacag cactgccccc cagctgaggg accagctcta
1080cttccacctg gagttgcaca gtctcaggct gggggcctca ggagaggtca
cagcccctca 1140gtctcttctc cttcccctgc ctgcaacagg ctgcctgccc
cgccttcccc aacacctcgc 1200tccatatgat agagcgtggc agctgggagc
aggcccctgc ccgtggtggg cccctaaagc 1260aatagcaccg taggccccct
gccctcttag cacaagaggc ccaggccctg gcctggcctt 1320cgtgcccttt
attcattgtc aataaatccg ctcagaccat taaaaaatac aactcaaggg
1380gtagccaaaa aaaaaaa 13971151581DNAHomo sapiensmisc_featureIncyte
ID No 182532 115acagcacagc tgacagccgt actcaggaag cttctggtat
cctaggctta tctccacaga 60ggagaacaca caagcagcag agaccatggg gcccctctca
gcccctccct gcacacacct 120catcacttgg aagggggtcc tgctcacagc
atcactttta aacttctgga atccgcccac 180aactgcccaa gtcacgattg
aagcccagcc acccaaagtt tctgagggga aggatgttct 240tctacttgtc
cacaatttgc cccagaatct tgctggctac atttggtaca aagggcaaat
300gacatacgtc taccattaca ttatatcgta tatagttgat ggtaaaataa
ttatatatgg 360gcctgcatac agtggaagag aaagagtata ttccaatgca
tccctgctga tccagaatgt 420cacgcaggag gatgcaggat cctacacctt
acacatcata aagcgaggtg atgggactag 480aggagaaact ggacatttca
ccttcacctt atacctggag actcccaagc cctccatctc 540cagcagcaac
ttatacccca gggaggacat ggaggctgtg agcttaacct gtgatcctga
600gactccggac gcaagctacc tgtggtggat gaatggtcag agcctcccta
tgactcacag 660cttgcagttg tccaaaaaca aaaggaccct ctttctattt
ggtgtcacaa agtacactgc 720aggaccctat gaatgtgaaa tacggaaccc
agtgagtggc atccgcagtg acccagtcac 780cctgaatgtc ctctatggtc
cagacctccc cagcatttac ccttcattca cctattaccg 840ttcaggagaa
aacctctact tgtcctgctt cgccgagtct aacccacggg cacaatattc
900ttggacaatt aatgggaagt ttcagctatc aggacaaaag ctctttatcc
cccaaattac 960tacaaagcat agtgggctct atgcttgctc tgttcgtaac
tcagccactg gcatggaaag 1020ctccaaatcc atgacagtca aagtctctgc
tccttcagga acaggacatc ttcctggcct 1080taatccatta tagcagccgt
gatgtcattt ctgtatttca ggaagactgg cagacagttg 1140ctttcattct
tcctcaaagt atttaccatc agctacagtc caaaattgct ttttgttcaa
1200ggagatttat gaaaagactc tgacaaggac tcttgaatac aagttcctga
taacttcaag 1260atcataccac tggactaaga actttcaaaa ttttaatgaa
caggctgata cttcatgaaa 1320ttcaagacaa agaaaaaaac ccaattttat
tggactaaat agtcaaaaca atgttttcat 1380aattttctat ttgaaaatgt
gctgattctt tgaatgtttt attctccaga tttatgcact 1440ttttttcttc
agcaattggt aaagtatact tttgtaaaca aaaattgaaa catttgcttt
1500tgctccctaa gtgccccaga attgggaaac tattcatgag tattcatatg
tttatggtaa 1560taaagttatc tgcacaagtt c 15811161566DNAHomo
sapiensmisc_featureIncyte ID No 239589 116cggctcgagt atggatctcc
aaggaagagg ggtccccagc atcgacagac ttcgagttct 60cctgatgttg ttccatacaa
tggctcaaat catggcagaa caagaagtgg aaaatctctc 120aggcctttcc
actaaccctg aaaaagatat atttgtggtg cgggaaaatg ggacgacgtg
180tctcatggca gagtttgcag ccaaatttat tgtaccttat gatgtgtggg
ccagcaacta 240cgtagatctg atcacagaac aggccgatat cgcattgacc
cggggagctg aggtgaaggg 300ccgctgtggc cacagccagt cggagctgca
agtgttctgg gtggatcgcg catatgcact 360caaaatgctc tttgtaaagg
aaagccacaa catgtccaag ggacctgagg cgacttggag 420gctgagcaaa
gtgcagtttg tctacgactc ctcggagaaa acccacttca aagacgcagt
480cagtgctggg aagcacacag ccaactcgca ccacctctct gccttggtca
cccccgctgg 540gaagtcctat gagtgtcaag ctcaacaaac catttcactg
gcctctagtg atccgcagaa 600gacggtcacc atgatcctgt ctgcggtcca
catccaacct tttgacatta tctcagattt 660tgtcttcagt gaagagcata
aatgcccagt ggatgagcgg gagcaactgg aagaaacctt 720gcccctgatt
ttggggctca tcttgggcct cgtcatcatg gtaacactcg cgatttacca
780cgtccaccac aaaatgactg ccaaccaggt gcagatccct cgggacagat
cccagtataa 840gcacatgggc tagaggccgt taggcaggca ccccctattc
ctgctccccc aactggatca 900ggtagaacaa caaaagcact tttccatctt
gtacacgaga tacaccaaca tagctacaat 960caaacaggcc tgggtatctg
aggcttgctt ggcttgtgtc catgcttaaa cccacggaag 1020ggggagactc
tttcggattt gtagggtgaa atggcaatta ttctctccat gctggggagg
1080aggggaggag ggtctcagac agctttcgtg ctcatggtgg cttggctttg
actctccaaa 1140gagcaataaa tgccacttgg agctgtatct ggccccaaag
tttagggatt gaaaacatgc 1200ttctttgagg aggaaacccc tttaggttca
gaagaatatg gggtgctttg ctcccttgga 1260cacagctggc ttatcctata
cagttgtcaa tgcacacaga atacaacctc atgctccctg 1320cagcaagacc
cctgaaagtg attcatgctt ctggctggca ttctgcatgt ttagtgattg
1380tcttgggaat gtttcactgc tacccgcatc cagcgactgc agcaccagaa
aacgactaat 1440gtaactatgc agagttgttt ggacttcttc ctgtgccagg
tccaagtcgg gggacctgaa 1500gaatcaatct gtgtgagtct gtttttcaaa
atgaaataaa acacactatt ctctggcaaa 1560aaaaaa 15661171815DNAHomo
sapiensmisc_featureIncyte ID No 1671302 117tttgtttctc ttattcccag
gacatcaagg agactttcaa taggtgtgaa gaggtacagc 60tgcagccccc agaggtctgg
tcccctgacc cgtgccaacc ccatagccat gacttcctga 120cagatgccat
cgtgaggaaa atgagccgga tgttctgtca ggctgcgaga gtggacctga
180cgctggaccc tgacacggct cacccggccc tgatgctgtc ccctgaccgc
cggggggtcc 240gcctggcaga gcggcggcag gaggttgctg accatcccaa
gcgcttctcg gccgactgct 300gcgtactggg ggcccagggc ttccgctccg
gccggcacta ctgggaggta gaggtgggcg 360ggcggcgggg ctgggcggtg
ggtgctgccc gtgaatcaac ccatcataag gaaaaggtgg 420gccctggggg
ttcctccgtg ggcagcgggg atgccagctc ctcgcgccat caccatcgcc
480gccgccggct ccacctgccc cagcagcccc tgctccagcg ggaagtgtgg
tgcgtgggca 540ccaacggcaa acgctatcag gcccagagct ccacagaaca
gacgctgctg agccccagtg 600agaaaccaag gcgctttggt gtgtacctgg
actatgaagc tgggcgcctg ggcttctaca 660acgcagagac tctagcccac
gtgcacacct tctcggctgc cttcctgggc gagcgtgtct 720ttcctttctt
ccgggtgctc tccaagggca cccgcatcaa gctctgccct tgattatcct
780gccacccgca gggcccctct gtcagcactt ggggggtggg tggtggaggg
tggcccgtaa 840gtttgagggc tcaaaggctc ttcccactgc ttgttactgt
gttgcttccc actccccctt 900gaccccaggc ccctgcttct ccctctagga
gcctaaagaa ccctcctggc ctccagctca 960gccttctctc acctactatg
tctgtccaac aggtctgcat gggtccctga taatgagaac 1020agctgcctgg
tcttctctcc cagtctgcct agcccagccc tgggactgga atttgagtag
1080gggatgaggg gaaattgtaa tttcattcct taacttcctt ttccccaccc
ctgctcttca 1140acctctttat cagttctgag gctggagggt ttgggcaagg
caacatcccc attccaattc 1200cattttctga tgcagatttt agctgaggga
tttggaagcc atttggggag gcaggctggg 1260ccaaagggta gagctgggta
ataaatgtct attctcctgg ggaggaggga ttctaaactt 1320tccttccgtc
ctcaatttct acctccatag accggccaga atttagcttc acttgagaga
1380gatctggaat ggtcgccatg attgaaacca cgcaccatta catcatcatt
acattaatta 1440catcaacata aattatttct tcccccttcc cttttccagc
actcaaccaa ggagcaaagc 1500tcatcccacc ccacacccct cccaggtctg
ctcactgcca ggctcctctc ccctttgttc 1560agtggagctg gcttttctcc
cagccccttt ccatgccttt cactccattt ggcaagctct 1620gagggggagc
ctggggacgg gtttgggtcc ccaggaggag agccttgggt ataatctatt
1680tttctaggag cctcttgcct tgtcacttgc agctttcgcc ctctgctttg
atggctgagg 1740tgaactcatg ttctttggga aaagggaagg cgtgctgtgg
aaataaaatg tttatttgct 1800tctctaaaaa aaaaa 18151181566DNAHomo
sapiensmisc_featureIncyte ID No 2041858 118caaagagcca ggctccagga
gaggaagggc tctgcgagag gagagaggag agcgctggag 60aggagaggct ggaggtgaga
gtcccaggaa aggcagagga gaatcgtagg gacataagtg 120tcccagcaca
ggcaaggagg aatccgagga taaggttctg gagggacaga agggcccaga
180gagagtcctt agccaggatg gaggctgttg tgaacttgta ccaagaggtg
atgaagcacg 240cagatccccg gatccagggc taccctctga tggggtcccc
cttgctaatg acctccattc 300tcctgaccta cgtgtacttc gttctctcac
ttgggcctcg catcatggct aatcggaagc 360ccttccagct ccgtggcttc
atgattgtct acaacttctc actggtggca ctctccctct 420acattgtcta
tgagttcctg atgtcgggct ggctgagcac ctatacctgg cgctgtgacc
480ctgtggacta ttccaacagc cctgaggcac ttaggatggt tcgggtggcc
tggctcttcc 540tcttctccaa gttcattgag ctgatggaca cagtgatctt
tattctccga aagaaagacg 600ggcaggtgac cttcctacat gtcttccatc
actctgtgct tccctggagc tggtggtggg 660gggtaaagat tgccccggga
ggaatgggct ctttccatgc catgataaac tcttccgtgc 720atgtcataat
gtacctgtac tacggattat ctgcctttgg ccctgtggca caaccctacc
780tttggtggaa aaagcacatg acagccattc agctgatcca gtttgtcctg
gtctcactgc 840acatctccca gtactacttt atgtccagct gtaactacca
gtacccagtc attattcacc 900tcatctggat gtatggcacc atcttcttca
tgctgttctc caacttctgg tatcactctt 960ataccaaggg caagcggctg
ccccgtgcac ttcagcaaaa tggagctcca ggtattgcca 1020aggtcaaggc
caactgagaa gcatggccta gataggcgcc cacctaagtg cctcaggact
1080gcaccttagg gcagtgtccg tcagtgccct ctccacctac acctgtgacc
aaggcttatg 1140tggtcaggac tgagcagggg actggccctc ccctccccac
agctgctcta cagggaccac 1200ggctttggtt cctcacccac ttcccccggg
cagctccagg gatgtggcct cattgctgtc 1260tgccactcca gagctggggg
ctaaaagggc tgtacagtta tttccccctc cctgccttaa 1320aacttgggag
aggagcactc agggctggcc ccacaaaggg tctcgtggcc tttttcctca
1380cacagaagag gtcagcaata atgtcactgt ggacccagtc tcactcctcc
accccacaca 1440ctgaagcagt agcttctggg ccaaaggtca gggtgggcgg
gggcctggga atacagcctg 1500tggaggctgc ttactcaact tgtgtcttaa
ttaaaagtga cagaggaaac cacggaaaaa 1560aaaaaa 15661191055DNAHomo
sapiensmisc_featureIncyte ID No 2198863 119tcagcagcca gcaaggtctt
tgagaaacac atggagctca ctgccctgct ccctttcagt 60ggcttcccat tgccttggat
aaagacccaa atgcctaaca gggcccataa ggccccacat 120gatccacggg
ctttagatgt gcagagatgt ggagcgcgat gccaggtagg gtgagcagtg
180gcgtggagca gggccacttg gctggggtgc caggtgttgg aggggagcag
cagcctgtcc 240acatggccta aggtttgagc tgggtgttgc tgctgggccg
ggcgagcgca gtgcagcgca 300ccgcggggag cgaggagcgc gcggaccggc
catgggcaag tcagcttcca aacagtttca 360taatgaggtc ctgaaggccc
acaatgagta ccggcagaag cacggcgtcc ccccactgaa 420gctctgcaag
aacctcaacc gggaggctca acagtattct gaggccctgg ccagcacgag
480gatcctcaag cacagcccgg agtccagccg tggccagtgt ggggagaacc
ttgcatgggc 540atcctatgat cagacaggaa aggaggtggc tgatagatgg
tacagtgaaa tcaagaacta 600taacttccag cagcctggct tcacctcggg
gactggacac ttcacggcca tggtatggaa 660gaacaccaag aagatgggcg
tggggaaggc gtccgcaagt gacgggtcct cctttgtggt 720ggccagatac
ttcccagcgg ggaatgttgt caatgagggc ttcttcgaag aaaacgtcct
780gccgccgaag aagtaacttg ttaaatgtaa tgggaaggtg gcagacttaa
gaacgtggat 840atgaagtgcc tagaaccacc acaacctggc tgtgcgtctg
tccctgtggg tgaatgtgct 900tgtgtgtgtg atgcatgtga gcgtctctgg
cacacacatt ggcatacagt tccgtgttcg 960cccatcttat tacaggagtg
agcaaaggaa gcatttaccc cgatggttac ctagaccacg 1020attaattgga
tnccccngaa anggggatcg gtttt 10551201956DNAHomo
sapiensmisc_featureIncyte ID No 3250703 120cactcaagga agatataaat
gacaaggtcg gctcagctct cagacaaggt tttccaagca 60agatgaagcc caacatcatc
tttgtacttt ccctgctcct catcttggag aagcaagcag 120ctgtgatggg
acaaaaaggt ggatcaaaag gccgattacc aagtgaattt tcccaatttc
180cacacggaca aaagggccag cactattctg gacaaaaagg caagcaacaa
actgaatcca 240aaggcagttt ttctattcaa tacacatatc atgtagatgc
caatgatcat gaccagtccc 300gaaaaagtca gcaatatgat ttgaatgccc
tacataagac gacaaaatca caacgacatc 360taggtggaag tcaacaactg
ctccataata aacaagaagg cagagaccat gataaatcaa 420aaggtcattt
tcacagggta gttatacacc ataaaggagg caaagctcat cgtgggacac
480aaaatccttc tcaagatcag gggaatagcc catctggaaa gggaatatcc
agtcaatatt 540caaacacaga agaaaggctg tgggttcatg gactaagtaa
agaacaaact tccgtctctg 600gtgcacaaaa aggtagaaaa caaggcggat
cccaaagcag ttatgttctc caaactgaag 660agctagtagc taacaaacaa
caacgtgaga ctaaaaattc tcatcaaaat aaagggcatt 720accaaaatgt
ggttgaagtg agagaggaac attcaagtaa agtacaaacc tcactctgtc
780ctgcgcacca agacaaactc caacatggat ccaaagacat tttttctacc
caagatgagc 840tcctagtata taacaagaat caacaccaga caaaaaatct
caatcaagat caacagcatg 900gccgaaaggc aaataaaata tcataccaat
cttcaagtac agaagaaaga cgactccact 960atggagaaaa tggtgtgcag
aaagatgtat cccaaagcag tatttatagc caaactgaag 1020agaaaataca
tggcaagtct caaaaccagg taacaattca tagtcaagat caagagcatg
1080gccataagga aaataaaata tcataccaat cttcaagtac agaagaaaga
catctcaact 1140gtggagaaaa gggcatccag aaaggtgtat ccaaaggcag
tatttcgatc caaactgaag 1200agcaaataca tggcaagtct caaaaccagg
taagaattcc tagtcaagct caagagtatg 1260gccataagga aaataaaata
tcataccaat cttcgagtac agaagaaaga cgtctcaaca 1320gtggagaaaa
ggatgtacag aaaggtgtat ccaaaggcag tatttctatc caaactgaag
1380agaaaataca tggcaagtct caaaaccagg taacaattcc tagtcaagat
caagagcatg 1440gccataagga aaataaaatg tcataccaat cttcaagtac
agaagaaaga cgactcaact 1500atggaggaaa gagcacgcag aaagatgtat
cccaaagcag tatttctttc caaattgaaa 1560agctagtaga aggcaagtct
caaatccaga caccaaatcc taatcaagat caatggtctg 1620gccaaaatgc
aaaaggaaag tctggtcaat ctgcagatag caaacaagac ctactcagtc
1680atgaacaaaa aggcagatac aaacaggaat ccagtgagtc acataatatt
gtaattactg 1740agcatgaggt tgcccaagat gatcatttga cacaacaata
taatgaagac agaaatccaa 1800tatctacata gccctgttgc ttagcaaccc
attgaaaagc tggaccaata gcaaggtgtc 1860accccgacct cagtgagtta
gggttcgttt ganccngant aggaangggt nccggaaggc 1920naaaannnnt
anttnagccn ctgttgtntn nanacc 19561211737DNAHomo
sapiensmisc_featureIncyte ID No 350287 121gaaatacagt ggctctttat
taaaaataat agttggataa tataaactga actatttatg 60catttttata tacttataaa
tccttccaaa tagttttaat tctatccttt tacatataaa 120taacttaata
agtgtgctgg aaaaacacag atgttcacag caccactgtt tttttttttt
180ttttttgaga taataaattc catgagaaat ctgggtttga atatttgttt
actttgtctc 240ctaattgaac accactccag gccttctgtc tgtctcccct
ttacccccaa aatattcaca 300aaaaaaattt taagacaaca agtaaccata
tataggtgtt tgaatgattt tctcattttt 360atctaatttc atttcataag
tcccgagtaa tttacctacc ataggctact atactgataa 420tataaatgaa
accgaacatt ttttgctact aactctcccc aatttaatgt gttttcgaaa
480taaaaattta aatttttttc cttttaatta aaaagtcatc tttgaagtcc
ttattggctg 540tacattttac atgtttgctg gtactattat tttgtcagtg
agttaaagct ggcatgtaca 600gctcttggct ttaatgaaaa gcacattgac
ataatgttag taaattccaa accccggcac 660agaatgtgag ttaaaattaa
gtcttgctgg gttagtgtac aataaactat acctacagac 720ttttttttaa
tagaaagaag acaaagctgc tggtatagga tttgttcctt tgaagaaaaa
780atgagggaaa caaacacaaa aacctcaatg cagtgtataa ataacatttt
gttcaactac 840ctcttaatgt ggaattatct actttaatag tttcctgaca
gtaatgttaa atagtaactg 900ccaaatttgt tattttccca tctctcttaa
aaaagtcttt atgattattt tatatagttt 960tgagaacttt aaagccactt
ttttttaacc ttacatttgc ataaaaatgt ttagctttta 1020agtagagagc
aaattatgat catatatttt gatattcatg acctgtttga ctataggatt
1080ttttttaaaa aaatgcactt tggctataaa accatggatg atttgatcca
taagatttaa 1140atgtgccacc attatagtat tcctagacat gagcttgatg
aatggtattc tgtaattata 1200acgtgccaca cattattgtg tcttaattgc
ccttagcctg aattttaatg atcaatttgt 1260tattgttgca gatgtgaata
ttgtgcataa acttactaaa tttatgtaaa attgtataaa 1320atagaattag
aagtcactaa gttctttctg tgtagaagta ataaatttat tgtaacacaa
1380tgcagttgtg tatatgacat tctgtaattc cttgaactgg atcatatatt
cataagttct 1440gtagatactt atgcatgaac attttctcat ttagttcttg
ggttcattat ttgtattgtg 1500tttactactt gtgatcatgt agttgtgcct
tactttgtga gaaaggttag ctcagtaaat 1560actgcaattt ctaaactcag
tgattggaag gttattaatt ataaatgtaa ctgataaagt 1620acgtgacagc
atttaaatct gtataaagaa caatggaagg atccttattg aattgttgct
1680tttttttaat atgtttaaat attatattaa aaacatttct ttctaaaaaa aaaaaaa
1737122789DNAHomo sapiensmisc_featureIncyte ID No 1618171
122caagatataa agtagcagtt ggctacctaa aatgaaaaga gcaatgttcc
atggcacctg 60aaatgttaaa aatattagaa actctcccca ccccatattc ctcccacccc
aattgagtct 120ctcgcaataa tcttctcgct tctctaacta gttgactttc
attatggatg gggataggct 180aaaaaacggg cccctgggat ggctgtgctg
ccatcagtgc tgttggttta ctcactcttt 240ttctgtcttc gtttttgcat
gctactgctc ctgccctctt acagccacag tagaagcggt 300agaggcccgg
gaaggtatgg ccatattact ctgatagatg tgatccatgt gtctgtgtac
360tggtttttcg aagctttatc aacatttcaa atattttatt attgcatcac
cagaactata 420acagtgagaa aaggtatagt tgtttctagg catgttaacg
aagcaggtgt ttcctttgtg 480tcctatcttt gcattaattt taaataacct
tcaccacagc tacagttttt tttctgggct 540ctatcagctt taatgcaacg
gcagaagctt aagcaactgg tcatgagagg tcaagtggtt 600tacttctgta
tcccttccat gtacaagaga catccatttg attctcaaga gagccaaata
660ggtcagcctc ttcagcgatt ctaaaagatt tcaagagcag aggcaggaag
taggactggg 720aatttagttc aattcattat ctgaggttgc cctaaggtag
ggcaagttta aatttaactt 780tgtttctat 7891231116DNAHomo
sapiensmisc_featureIncyte ID No 1625863 123tttatatttg acaataaagt
gttagactcc atttctaaat accagacttc aaaagataag 60gttcaaaagt gttataagaa
gatattcctt tttttgtcct agagaactta ttttcctgtg 120aaaatgccta
ccacaaagaa gacattgatg ttcttatcaa gctttttcac cagccttggg
180tccttcattg taatttgctc tattcttggg acacaagcat ggatcaccag
tacaattgct 240gttagagact ctgcttcaaa tgggagcatt ttcatcactt
acggactttt tcgtggggag 300agtagtgaag aattgagtca cggacttgca
gaaccaaaga aaaagtttgc agttttagag 360atactgaata attcttccca
aaaaactctg cattcggtga ctatcctgtt cctggtcctg 420agtttgatca
cgtcgctgct gagctctggg tttaccttct acaacagcat cagcaaccct
480taccagacat tcctggggcc gacgggggtg tacacctgga acgggctcgg
tgcatccttc 540gtttttgtga ccatgatact gtttgtggcg aacacgcagt
ccaaccaact ctccgaagag 600ttgttccaaa tgctttaccc ggcaaccacc
agtaaaggaa cgacccacag ttacggatac 660tcgttctggc tcatactgct
cgtcattctt ctaaatatag tcactgtaac catcatcatt 720ttctaccaga
aggccagata ccagcggaag caggagcaga gaaagccaat ggaatatgct
780ccaagggacg gaattttatt ctgaattctc tttcatctca ttttggcgtt
gcatctattg 840tacatcagcc ctgagtagta actggttagc ttctctggac
aattcagcat ggtaacgtga 900ctgtcatctg tgacagcatt tgtgtttcat
gacactgtgt tcttcattga tgctgtactc 960ctgaaaattt ttcccacaag
gttggggaaa tgaatgggaa atgtcgctgg tctgtgtggt 1020attcaaagca
gtagtatcat gatgagcgta acgacccttc tgacctggtc tcacgatctg
1080aaataataaa aggctgtgtc atgtttcttt tcaaaa 1116124914DNAHomo
sapiensmisc_featureIncyte ID No 1638353 124ggccaaccca cggtgggggg
agcgcggcca tggcgctcct gctttcggtg ctgcgtgtac 60tgctgggcgg cttcttcgcg
ctcgtggggt tggccaagct ctcggaggag atctcggctc 120cagtttcgga
gcggatgaat gccctgttcg tgcagtttgc tgaggtgttc ccgctgaagg
180tatttggcta ccagccagat cccctgaact accaaatagc tgtgggcttt
ctggaactgc 240tggctgggtt gctgctggtc atgggcccac cgatgctgca
agagatcagt aacttgttct 300tgattctgct catgatgggg gctatcttca
ccttggcagc tctgaaagag tcactaagca 360cctgtatccc agccattgtc
tgcctggggt tcctgctgct gctgaatgtc ggccagctct 420tagcccagac
taagaaggtg gtcagaccca ctaggaagaa gactctaagt acattcaagg
480aatcctggaa gtagagcatc tctgtctctt tatgccatgc agctgtcaca
gcaggaacat 540ggtagaacac agagtctatc atcttgttac cagtataata
tccagggtca gccagtgttg 600aaagagacat tttgtctacc tggcactgct
ttctcttttt agctttacta ctcttttgtg 660aggagtacat gttatgcata
ttaacattcc tcatgtcata tgaaaataca aaataagcag 720aaaagaaatt
taaatcaacc aaaattctga tgccccaaat aaccactttt aatgccttgg
780tgtaagtata cctctgaact tttttctgtg cctttaaaca gatatatatt
ttttttaaat 840gaaaataaaa ccatatatcc tattttattt cctcctttta
aaaccttata aactataaca 900ctgtaaaaaa aaaa 9141252016DNAHomo
sapiensmisc_featureIncyte ID No 1726843 125gctgcctgct gcctccgcag
cgtcccccca gctctccctg tgctaactgc ctgcaccttg 60gacagagcgg gtgcgcaaat
cagaaggatt agttgggacc tgccttggcg accccatggc 120atcccccaga
accgtaacta ttgtggccct ctcagtggcc ctgggactct tctttgtttt
180catggggact atcaagctga cccccaggct cagcaaggat gcctacagtg
agatgaaacg 240tgcttacaag agctatgttc gagccctccc tctgctgaag
aaaatgggga tcaattccat 300tctcctccga aaaagcattg gtgcccttga
agtggcctgt ggcatcgtca tgacccttgt 360gcctgggcgt cccaaagatg
tggccaactt cttcctactg ttgctggtgt tggctgtgct 420cttcttccac
cagctggtcg gtgatcctct caaacgctac gcccatgctc tggtgtttgg
480aatcctgctc acttgccgcc tgctgattgc tcgcaagccc gaagaccggt
cttctgagaa 540gaagcctttg ccagggaatg ctgaggagca accctcctta
tatgagaagg cccctcaggg 600caaagtgaag gtgtcataga aaagtggaag
tgcaaagagt ggaccttcca ggcagttgcg 660tccatgacac caggaagatg
tcagtgtgtg tttttcattt gatttattta tcttggggaa 720agtgaaaaat
gtaatctgca agttaatgac cctattggct tgtgtacatc tatatgctaa
780aatgacttcc ccacattgac atttgtgcgc cacctttaat cactctgggg
caactctcac 840atcttgctgc atgtacatgt atacggctac tattgaagtg
taattgtgag atggactcca 900acaagcatgt gactgtgaga ttgtgtgtgg
gaaaatgtat ttaactactc tgtgtgtgtg 960tgtgtgtgtg tgtgtgcgcg
cgcgcgcacg cgcacacact cacgcacaca caagcagaga 1020aggcgctgat
cttgaactaa tcctgcacag gcatccttcc ctttatagat tgattccagc
1080aaaggcggaa taaaacaaat ttcctatgaa gagaatcctg atatgaaaca
agtcatgtag 1140tctcatggcc gggaatctct ccacagatac taacaactta
aacttactac tttaggagaa 1200aaaaaaaaac attcaatttc ggacactgag
ttatatatga aattaattag gctctagtcc 1260aacagttgtt tacattttaa
atagtccata ttgaatttaa ttaaaacaag ggatgcatgc 1320agtcaaattg
atagtttaat tcttcaagtg ataatatgga agtttcacct tgcctttgtc
1380caagccccac ctattaaaac cctttactca cagtttgaaa ctgaagcagt
aaacttgttt 1440ccagacatct ttttcagatt gtcttaagcc caaagttgcc
tcacttccac tattctcagc 1500agccaaccag gatttggcag ctgctccact
gttacggttg agggaacagg gatcagtcct 1560gttagaagtc tgtgagcctc
aaactctacc tgttctctgc aatcatccaa aatttgaaaa 1620agaagctata
tccagtgttt cactgccaaa cagattcact actcttactg attcttcact
1680gagctttgct agtataagca gagttccaag tctcccctag ggttgtctct
acatttcttt 1740atcattccag tgggtagggt ttagctgggg gaaggacatt
tcataagggt tagttggact 1800gagcagtatg gacatttgct tttttcatta
cgtactgttg tttttccttg ttaggtgtgc 1860tttggtggtt ttaatattat
tgtgccaggg atggggaaat ggggggggtt gtgtgggaag 1920agtacttatt
attgtgtttt cttcagtgta attgttcttg gtaattgata cctctctgtt
1980ttatttctct cattctttca aaataaaact ttttgt 20161262067DNAHomo
sapiensmisc_featureIncyte ID No 1754506 126tgctccttta agcgtccaca
ggcggcggac ggccacaatc acagctccgg gcattggggg 60aacccgagcc ggctgcgccg
ggggaatccg tgcgggcgcc ttccgtcccg gtcccatcct 120cgccgcgctc
cagcacctct gaagttttgc agcgcccaga aaggaggcga ggaaggaggg
180agtgtgtgag aggagggagc aaaaagctca ccctaaaaca tttatttcaa
ggagaaaaga 240aaaagggggg gcgcaaaaat ggctggggca attatagaaa
acatgagcac caagaagctg 300tgcattgttg gtgggattct gctcgtgttc
caaatcatcg cctttctggt gggaggcttg 360attgctccag ggcccacaac
ggcagtgtcc tacatgtcgg tgaaatgtgt ggatgcccgt 420aagaaccatc
acaagacaaa atggttcgtg ccttggggac ccaatcattg tgacaagatc
480cgagacattg aagaggcaat tccaagggaa attgaagcca atgacatcgt
gttttctgtt 540cacattcccc tcccccacat ggagatgagt ccttggttcc
aattcatgct gtttatcctg 600cagctggaca ttgccttcaa gctaaacaac
caaatcagag aaaatgcaga agtctccatg 660gacgtttccc tggcttaccg
tgatgacgcg tttgctgagt ggactgaaat ggcccatgaa 720agagtaccac
ggaaactcaa atgcaccttc acatctccca agactccaga gcatgagggc
780cgttactatg aatgtgatgt ccttcctttc atggaaattg ggtctgtggc
ccataagttt 840taccttttaa acatccggct gcctgtgaat gagaagaaga
aaatcaatgt gggaattggg 900gagataaagg atatccggtt ggtggggatc
caccaaaatg gaggcttcac caaggtgtgg 960tttgccatga agaccttcct
tacgcccagc atcttcatca ttatggtgtg gtattggagg 1020aggatcacca
tgatgtcccg acccccagtg cttctggaaa aagtcatctt tgcccttggg
1080atttccatga cctttatcaa tatcccagtg gaatggtttt ccatcgggtt
tgactggacc 1140tggatgctgc tgtttggtga catccgacag ggcatcttct
atgcgatgct tctgtccttc 1200tggatcatct tctgtggcga gcacatgatg
gatcagcacg agcggaacca catcgcaggg 1260tattggaagc aagtcggacc
cattgccgtt ggctccttct gcctcttcat atttgacatg 1320tgtgagagag
gggtacaact cacgaatccc ttctacagta tctggactac agacattgga
1380acagagctgg ccatggcctt catcatcgtg gctggaatct gcctctgcct
ctacttcctg 1440tttctatgct tcatggtatt tcaggtgttt cggaacatca
gtgggaagca gtccagcctg 1500ccagctatga gcaaagtccg gcggctacac
tatgaggggc taatttttag gttcaagttc 1560ctcatgctta tcaccttggc
ctgcgctgcc atgactgtca tcttcttcat cgttagtcag 1620gtaacggaag
gccattggaa atggggcggc gtcacagtcc aagtgaacag tgcctttttc
1680acaggcatct atgggatgtg gaatctgtat gtctttgctc tgatgttctt
gtatgcacca 1740tcccataaaa actatggaga agaccagtcc aatggaatgc
aactcccatg taaatcgagg 1800gaagattgtg ctttgtttgt ttcggaactt
tatcaagaat tgttcagcgc ttcgaaatat 1860tccttcatca atgacaacgc
agcttctggt atttgagtca acaaggcaac acatgtttat 1920cagctttgca
tttgcagttg tcacagtcac attgattgta cttgtatacg cacacaaata
1980cactcattta gcctttatct caaaatgtta aatataagga aaaaagcgtc
aacaataaat 2040attctttgag tattgaaaaa aaaaaaa 20671272180DNAHomo
sapiensmisc_featureIncyte ID No 1831378 127gcgaacgtct gcacctggcg
ggcgatgacg cccgatgcgg gcgccccggg atagcgtggg 60cgaggctgcg gggccccggc
gcgcacgccc gcacctctcc ccagccctgg cgtgggccca 120gcccggccca
ggcagcaatg gggttcctgc agctgctggt cgtagcggtg ctggcatccg
180aacaccgggt ggctggtgca gccgaggtct tcgggaattc cagcgagggt
cttattgaat 240tttctgtggg gaaatttaga tacttcgagc tcaataggcc
ctttccagag gaagctattt 300tgcatgatat ttcaagcaat gtgacttttc
ttattttcca aatacactca cagtatcaga 360atacaactgt ttccttttct
ccgactctcc tttccaattc ctcggaaaca ggcactgcca 420gtggactggt
tttcatcctt agaccagagc agagtacatg cacttggtac ttggggactt
480caggcataca gcctgtccag aatatggcta tcctactctc ctactcagaa
agagatcctg 540tccctggagg ctgtaatttg gagttcgatt tagatattga
tcccaacatt tacttggagt 600ataatttctt tgaaacgact atcaagtttg
ccccagcaaa cctaggctat gcgagaggcg 660tagatccccc accatgtgac
gctgggacag accaggactc caggtggagg ttgcagtatg 720atgtctatca
gtattttctg cctgagaatg acctcactga ggagatgttg ctgaagcatc
780tgcagaggat ggtcagtgtg ccccaggtga aggccagtgc tctcaaggtg
gttaccctaa 840cagctaatga taagacaagt gtttccttct cctccctccc
gggacaaggt gtcatataca 900atgtcattgt ttgggacccg tttctaaata
catctgctgc ctacattcct gctcacacat 960acgcttgcag ctttgaggca
ggagagggta gttgtgcttc cctaggaaga gtgtcttcca 1020aagtgttctt
cactcttttt gccctgcttg gtttcttcat ttgtttcttt ggacacagat
1080tctggaaaac agaattattc ttcataggct ttatcatcat gggattcttc
ttttatatac 1140tgattacaag actgacacct atcaagtatg atgtgaatct
gattctgaca gctgtcactg 1200gaagcgtcgg tggaatgttc ttggtagctg
tgtggtggcg atttggaatc ctctcgatct 1260gcatgctctg tgttggacta
gtgctggggt tcctcatctc gtcagtgact ttctttactc 1320cactgggaaa
cctaaagatt tttcatgatg atggtgtatt ctgggtcact ttctcttgca
1380tagctatcct cattccagta gttttcatgg gctgcctaag aatactgaac
atactgactt 1440gtggagtcat tggctcctat tcggtggttt tagccattga
cagttactgg tccacaagcc 1500tttcctacat cactttgaac gtactcaaga
gagcgctcaa caaggatttc cacagagctt 1560tcacaaatgt gccttttcaa
actaatgact tcattatcct ggcagtatgg ggcatgctgg 1620ctgtaagtgg
aattacgtta cagattcgaa gagagagagg acgaccgttc ttccctcccc
1680acccatacaa gttatggaag caagagagag agcgccgagt gacaaacatt
ctggacccta 1740gctaccacat tcctccattg agagagaggc tctatggccg
attaacccag attaaagggc 1800tcttccagaa ggagcagcca gctggagaga
gaacgccttt gcttctgtag atgcccaggg 1860gcttggtcag tgtgcctcag
ctttggagtt catgcctgga gtggttcaac agtctctggt 1920gcaagtctaa
taagagatca ggcatatata tctgttcttt gcataatatt atggtgccct
1980tattgatata tggtaagggt gtactagggg attaggatga ttgtaagaga
atgagaaaga 2040tgaccaaaag gttggtggta gggaggcttt ttcttatttc
caaatacttg agaaattacc 2100ttttggttta caaatctatg atcaacttat
tccattaaat agatacatta aaaaaattaa 2160aaactgaaaa aaaaaaaaaa
2180128991DNAHomo sapiensmisc_featureIncyte ID No 1864943
128cacggtgtca gcaggcaaca tggccgagag gcggggcctc cgggcggcgc
cgtgtccgcg 60accgcgtacc ctgacacccc cgcggaattc cctccgcacc tccaggcggg
tgcgatgcgg 120cgccgctttt ggggcgtatt caactgtctg tgcgccggcg
cgttcggggc cctggccgcc 180gcctccgcca agctggcctt cggcagcgag
gtgagcatgg gtttatgcgt cttaggcatt 240attgtgatgg cgagcaccaa
ttctctgatg tggaccttct ttagccgggg cctcagtttc 300tccatgtctt
cagccattgc atctgtcaca gtgacttttt caaatatcct cagctcggcc
360ttcctgggct atgtgctgta tggagagtgc caggaggtct tgtggtgggg
aggagtgttc 420cttattctct gcggactcac cctaatccac aggaagctcc
cacccacctg gaagcccctt 480ccacacaagc agcagtagca ccacttggct
agacggacca gctggaaaga tcatgatggt 540ggcccagcct tgggatgtca
tgtgggactg tgtcctaggg cgatccagtt gtgcagcctt 600ctgaccatca
gccaagggaa gcaggcctct gatggagcag gctctggctc tgtaaggaga
660ggtgcagctg cagcagtgtt ctaccggaag tgttttgatc atctgtacag
tgctttggat 720tcttcctccc aggcctaccc cagtgagcct tcgcagatgc
tggagatcct ggggttggtc 780tgctttgtgt atggtacttg aaaccacgct
gtaattattg tcctgttgcc aaacaaaagc 840cagtcatgta actctagaag
cagtgactgg tggggctttc tgacagttcc atgctgatgt 900atcaggccat
ctgtgtcatg cttatgtatt atggcaagaa gaggaaaact ggattaataa
960atacgttttt ttgtaagtta aaaaaaaaaa a 991129637DNAHomo
sapiensmisc_featureIncyte ID No 1911316 129ggagggcggt gctccgccgc
ggtggcggtt gctatcgctt cgcagaacct actcaggcag 60ccagctgaga agagttgagg
gaaagtgctg ctgctgggtc tgcagacgcg atggataacg 120tgcagccgaa
aataaaacat cgccccttct gcttcagtgt gaaaggccac gtgaagatgc
180tgcggctggc actaactgtg acatctatga ccttttttat catcgcacaa
gcccctgaac 240catatattgt tatcactgga tttgaagtca ccgttatctt
atttttcata cttttatatg 300tactcagact tgatcgatta atgaagtggt
tattttggcc tttgcttgat attatcaact 360cactggtaac aacagtattc
atgctcatcg tatctgtgtt ggcactgata ccagaaacca 420caacattgac
agttggtgga ggggtgtttg cacttgtgac agcagtatgc tgtcttgccg
480acggggccct tatttaccgg aagcttctgt tcaatcccag cggtccttac
cagaaaaagc 540ctgtgcatga aaaaaaagaa gttttgtaat tttatattac
tttttagttt gatactaagt 600attaaacata tttctgtatt cttccaaaaa aaaaaaa
6371302631DNAHomo sapiensmisc_featureIncyte ID No 1943120
130ctctcttcct gcagtgtggt aaaactacag caatcgtctt aacctgtgag
atctgtcacc 60tttgcatttt ccactcatgc agctggttct ataaaccaac tcttctgctt
ggggggatct 120aatcatgacc ttttaccctt ttgtggcctc ttctagtaca
aggcgagtgg ataattccaa 180cacaagactg gcagtccaaa ttgaaagaga
tccagggaat gatgacaaca atctcaattc 240cattttttat gaacacttga
caaggaccct cctggagtcc ctctgtggag acttagttct 300tggacgttgg
ggcaactaca gctctggcga ttgctttatt ttggcttcag atgacctcaa
360tgcctttgtt cacctgattg aaattggaaa tggtcttgtc acctttcaac
ttcgaggact 420ggaattccga ggaacctact gccagcagag ggaggtagaa
gccatcatgg agggcgacga 480ggaggacaga ggctgctgct gctgcaaacc
aggccacttg cctcacctgc tgtcccgcaa 540cgctgccttt cacctccgct
ggctcacctg ggaaatcacg cagacccagt acatcctgga 600gggctacagc
atcctggaca acaacgcggc caccatgctg caggtgtttg acctccgaag
660gatcctcatc cgctactaca tcaagagtat aatatactat atggtaacgt
ctcccaaact 720cctctcctgg atcaaaaatg aatcacttct gaagtccctg
cagccctttg ccaagtggca 780ttacattgag cgtgaccttg caatgttcaa
cattaacatt gatgatgact acgtcccgtg 840tctccagggg atcacacgag
ctagcttctg caatgtttat ctagaatgga ttcaacactg 900tgcacggaaa
agacaagagc cttcaacgac cctggacagt gacgaggact ctcccttggt
960gactctgtcc ttcgccctgt gcaccctggg gaggagagct ctgggaacag
ccgctcacaa 1020tatggccatc agcctggatt ctttcctgta tggcctccat
gtcctcttca aaggtgactt 1080cagaataaca gcacgtgacg agtgggtatt
tgctgacatg gacctactgc ataaagttgt 1140agctccagct atcaggatgt
ccctgaaact tcaccaggac cagttcactt gccctgacga 1200gtatgaagac
ccagcagtcc tctacgaggc catccagtcc ttcgagaaga aggtggtcat
1260ctgccacgag ggcgacccgg cctggcgggg cgcagtgctg tccaacaagg
aagagctgct 1320caccctgcgg cacgtggtgg acgagggtgc cgacgagtac
aaggtcatca tgctccacag 1380aagcttcctg agcttcaagg tgatcaaggt
taacaaagaa tgcgtccgag gactttgggc 1440cgggcagcag caggagctta
tatttcttcg caaccgcaat ccggagcgcg gcagtatcca 1500gaacaataag
caggtcctgc ggaacttgat taactcctcc tgcgatcagc ccctggggta
1560ccccatgtat gtctccccac taaccacatc ctacctaggg acacacaggc
agctgaagaa 1620catctggggt ggacccatca ctttggacag aattaggacc
tggttctgga ccaagtgggt 1680aaggatgcgg aaggattgca atgcccgcca
gcacagtggc ggcaacattg aagacgtgga 1740cggaggaggg gccccgacga
caggtggcaa caatgccccg aatggtggca gccaggagag 1800cagcgcagaa
cagcccagaa aaggcggtgc tcagcacggg gtgtcatcct gtgaagggac
1860acagagaaca ggcaggagga aaggcaggag ccagtccgtg caggcacact
cagcgctaag 1920ccaaaggccg cccatgctga gctcatctgg ccccatctta
gagagccgcc aaacattcct 1980ccagacgtcc acctcagtgc acgagctggc
ccagaggctc tcgggcagcc ggctctcctt 2040gcacgcctcg gccacgtccc
tgcactctca gcccccgccc gtcaccacca ccggccacct 2100gagtgtccgt
gagcgggccg aggcgctcat caggtccagc ctgggctcct ccaccagctc
2160caccctgagc ttcctcttcg gcaagaggag cttttccagc gcgctcgtca
tttccggact 2220ctctgctgcg gaggggggca ataccagtga cacccagtca
tccagcagcg tcaacatcgt 2280gatgggcccc tcagccaggg ctgccagcca
ggccactcgg gtaaggggct gggcagggct 2340caccaggaca ggctgggatg
gtggcacggg ctcctggcct gagcgtggca cctgccttgc 2400gttcccaccc
ttctgcctgc agaaccccat ccccttctct atggggctcc cagagtgaca
2460aaggacagtg attagacacg aagtggctta gctgctcttg aaagcagaca
agatacagag 2520cagatatcct gtaaacgata atgcccaggc aggcactgaa
aggagtcacc ggatacagag 2580gttctgcaga actgtggcca tctgccctac
accggggcat gacggagaat g 2631131646DNAHomo sapiensmisc_featureIncyte
ID No 2314236 131tacatttact aaaatgatgt aataaataac atgttaatag
actcaagctt taccttatga 60aattgatgta tttttaccag ttatttctaa tgtaacattg
aatatataag atctgacaaa 120tgtatgttta aacatgaatt agaagagttg
agaactacca ttatgtatag ggattctcat 180agtgtcttgg cccttaattg
gaaagttgtg gcaactttaa agtacttttt actgtatgtt 240ataattcttt
ataacttaga gagagacaat ggtcactcaa actatgagaa ctatgaatta
300ggagataaaa gtttaaattt gttgttgttt tataacagta tgtacaagtt
agttttccct 360tatatattta cgttttcaag ttttttaatc tcatcatata
catccatact ctataaaatg 420ttttatattc aaagaactgt aaaatcctaa
acattagttt tcactattga aattgttttt 480taaagatagg cataaatagt
tgtccttaga cttattcata caaatatagt catttacttc 540tatgtagttt
gagattctga gagttattcc aactttatga agattgattt caatgtgcct
600gctaagtcct aaaagattca gaaagaaaat ttatatatta ttgatt
646132541DNAHomo sapiensmisc_featureIncyte ID No 2479409
132ttcacatttt ttggtttgat cttggtgtca tttaggtaat gaatctatcc
aagaaatcta 60tccttttgac ccaggttatc aaatttgtag acataaggtt atttataatg
gtcccttctt 120acccttttaa tgtctttagg agctgtgttg ataatttcct
tttcattatg atactggtaa 180tttctgttct cactttccta atcaggttgg
gtaggggttt atcagtttta ctgatctgac 240tttttatttt attttatttt
ttttgagaca gtcttacact gtctcccagg ctggagtgca 300gtggcgcgat
ctcggcttac tgcaagctct gccttccggg ttcatgccat tctcctgcct
360cagcctcccc agtagctggg actacaggct cccacaacac gcccggctaa
ttttttaaat 420tcttagtgga gactggggtt caccggggta accaagaatg
gctcggatct ctttaacccc 480ggggtccacc cgcctcagcc tcccaaaagt
gctggggatt acaggggtga gcaccgggcc 540c 5411331922DNAHomo
sapiensmisc_featureIncyte ID No 2683149 133tggcctccga tccacctgga
cacctggagg ctaagcctgg attccccctt ccctgactca 60ggaactgctt aacgtctaca
gcaaggccta ataggggacc tgagggcaca gtcctcagga 120tgtttcgggg
agaataggag ccagaacctg agcccctaag ccattcccct caccaatgat
180ggggtcccca gtgagtcatc tgctggccgg cttctgtgtg tgggtcgtct
tgggctgggt 240agggggctca gtccccaacc tgggccctgc tgagcaggag
cagaaccatt acctggccca 300gctgtttggc ctgtacggcg agaatgggac
gctgactgca gggggcttgg cgcggcttct 360ccacagcctg gggctaggcc
gagttcaggg gcttcgcctg ggacagcatg ggcctctgac 420tggacgggct
gcatccccag ctgcagacaa ttccacacac aggccacaga accctgagct
480gagtgtggat gtctgggcag ggatgcctct gggtccctca gggtggggtg
acctggaaga 540gtcaaaggcc cctcacctac cccgtgggcc agccccctcg
ggcctggacc tgcttcacag 600gcttctgttg ctggaccact cattggctga
ccacctgaat gaggattgtc tgaacggctc 660ccagctgctg gtcaattttg
gcttgagccc cgctgctcct ctgacccctc gtcagtttgc 720tctgctgtgc
ccagccctgc tttatcagat cgacagccgc gtctgcatcg gcgctccggc
780ccctgcaccc ccaggggatc tactatctgc cctgcttcag agtgccctgg
cagtcctgtt 840gctcagcctc ccttctcccc tatccctgct gctgctgcgg
ctcctgggac ctcgtctact 900acggcccttg ctgggcttcc tgggggccct
ggcggtgggc actctttgtg gggatgcact 960gctacatctg ctaccgcatg
cacaagaagg gcggcacgca ggacctggcg gactaccaga 1020gaaggacctg
ggcccggggc tgtcagtgct cggaggcctc ttcctgctct ttgtgctgga
1080gaacatgctg gggcttttgc ggcaccgagg gctcaggcca agatgctgca
ggcgaaaacg 1140aaggaatctc gaaacacgca acttggatcc ggagaatggc
agtgggatgg cccttcagcc 1200cctacaggca gctccagagc caggggctca
gggccagagg gagaagaaca gccagcaccc 1260accagctctg gcccctcctg
ggcaccaagg ccacagtcat gggcaccagg gtggcactga 1320tatcacgtgg
atggtcctcc tgggagatgg tctacacaac ctcactgatg ggctggccat
1380aggtgctgcc ttctctgatg gcttctccag cggcctcagt accaccttag
cggtcttctg 1440ccatgagctg ccccacgaac tgggtgactt tgccatgctg
ctccagtcag ggctgtcctt 1500tcggcggctg ctgctgctga gcctcgtgtc
tggagccctg ggattggggg gtgcagtcct 1560gggggtgggg ctcagcctgg
gccctgtccc cctcactccc tgggtgtttg gggtcactgc 1620tggggtcttc
ctctatgtgg cccttgtgga catgctacca gccctgcttc gtcctccgga
1680gcccctgcct acgccccatg tgctcctgca ggggctgggg ctgctgctgg
ggggcggcct 1740catgcttgcc ataaccctgc tggaggagcg gctactgccc
gtgaccactg agggctgatg 1800gggccagtgg aaaggggtcg ggttgccctt
ccttcccccc aaccacagga atggaggcgg 1860gacacagggc cagtaggagc
aataggattt taataaacag aacccatccc aaaaaaaaaa 1920aa
1922134840DNAHomo sapiensmisc_featureIncyte ID No 2774051
134ggtaattcgt actggtcatc ttctctgggt gtgagtcaaa tataagttta
acaattagct 60ctgaaaacat tccattgagc tggggaatgc aacagtctta ttacctcatc
atggaattct 120ctagcttagt taatttaaat attgtttctt agtttctggg
tcaattaaat ttaaatgatg 180tagtttatgc ttcgtgacca attaaattac
taggttatta caaaaaaaat tatcatcttt 240tttgattaaa gagctgtggg
tacagtatat tttataagca attttcatta gttcaaaaat 300gttcctttag
gctagattaa gcagccattc attgttagag cctggagacc ttattcgaag
360gtgttcatcg tattcacagt gcactattac ttagaactaa agccaattga
acctacttag 420caatagcgtt atgcctttca cccttgatga ttatggagct
tatagctctc agaaacaata 480cacctgtcag tttccatcaa ctatagcaat
ccatgcagaa gacaagaggc cccctcaaag 540caggaggggt attgttttag
gtccaatttt tcttattgtt ctcaaaatca ttataaggtg 600gacagtgttt
tgtgaagatt ttcttttccc cagctctaag aaaccatgtg gaaagaattc
660attgataact gttttgattt ttttcttttt ttaagtacag gttttgctaa
gtaatcaccc 720ttagtgagcc tgtgtagttc agctgcctgt gagatgtttg
gtgaccagct cagtggtatc 780ttgtattcct gatagagaat atttcagggg
acanagtgct ctttcagaca gactcaaata 8401351344DNAHomo
sapiensmisc_featureIncyte ID No 2869038 135gcaaattgat ctaaaagcca
ctaataaatt ctagggtttg agtctagaag ccaagcaaac 60tgtcaccaat gtcagttgta
aattagaatg caacatgagg cttcagactc atgacaatga 120tatacatgaa
aacaaaaata taattgtgtc taccttccta ctttcccttt tgacatatgt
180agttggaatt ttacatagtc ttaaaatcca tatttagaat cttacctgtt
tctataataa 240ttagtaaaat gccaaagtag tgatagaata ttgtggcatt
gaagtagccg aaaaattgtt 300agttttagca tcaaaaaagt aaatagatgt
tgaaatgaat ttttgtatgt gccaggttga 360agagagtgtg ccagtgacag
gaagtagtct aaaaaattaa cagttatggt tttaatagga 420tctgaaagac
aatctttaaa gaaatgggag aaattggggg tatcagtgaa cctataccaa
480cctctctttg tacataaata tggtgatgta gctagatata aaaatcagtg
tcttactggc 540accatttaca gtttagaaaa caatcttttt cttaaaaatg
cccatctgat ttctattttt 600aggagctact tggatttgta tgtatttttt
ctacgtgaaa atatatgtac tcttcacttt 660tgttccagta ctataattgc
tcatgcactc tttctcccct ttgagaacat tcagtgaaat 720acaacttcat
caaagatttg ctcaaaggag aagaatcgca tgagtgtgaa aagtagatgc
780tcgtagccag aacagaaaag gttacacatg atcatggcac agaagatagg
aggtttgact 840tggtgggcca taatgtttat tatccttttt gaaataacag
ggaccagcag cagttttctc 900aggataaatg ctctacccca cttctctatg
aacaggtgtg gggaggctta ctttccattt 960tcatatttat acacctctct
acaaaagcaa tttttaatga aggttagtgg aattgttaaa 1020aatctgagag
gaatgatgac tggaggtgtt tggggttttt ttctgtattc attttttaat
1080gagaaaagtt ttaaatgtag tacaggttag acccaactac taccttacta
ttataggacg 1140attctatgtt tctgttaaag tattcaagta gctttctctg
ggggaaaaag taccacttgg 1200acacttaaag gaattgggat ttttgtctac
tttggataag gcagttgact tcttaagtaa 1260aagcaatagt gtaaaatgtc
attttgtttg gaatgttaag tgagcaaata aaaaacatgt 1320tgaaattgtt
gtaaaaaaaa aaaa 1344136443DNAHomo sapiensmisc_featureIncyte ID No
2918334 136ctcgagattt tttatattta tgcatgccat ttagtttgct cctaaaaata
gtgatactgg 60ttttagtttt ttacttacta aatcagtata gccaaatgtc catcttccta
gtggtaatat 120gcgatcagaa tttctgagat tatttatgtg actatttttg
gaaaagtttc ttttgataaa 180acatggattt attatatgaa attcttcttg
cactgtatta caatatatgc tatgatatcc 240cttttatttt tttcaactta
aatatgatgt tttatattgt tttagactta cgaatcgtgt 300ttttcagaac
cataagggaa
tatctatctc ctccctcact ttccttttac atatattgaa 360aagtctatga
aattcaagtc tagcatttga attctctatg ctatcattgc atttacctaa
420ttatttactt ttaaatttta ggg 443137467DNAHomo
sapiensmisc_featureIncyte ID No 2949916 137gccatttaag gagatctgtt
ttgcttgaat attctgactg tcagtccgca gacataggga 60gtgtgtgagt gtgagtgtgt
accaagatga ggaggataat caggctccgg ctccgttttt 120ctgacacttt
tatggctgcc tttcttctgt gcctgggctt cgttctcatg ctctttccct
180cgttgttgcg ggatggtggc agcatcagca gctgcagaaa ctcttgttca
tctcctagct 240ccgaggagcg tcatttctcc aacttggaat aaaagcccat
cctctacctg attgggccac 300tcagatcaag ggcttaacac tagcaacagt
tgctaaggca ctgctagata ccgattagct 360gaagcctggg tgtctgaacc
aatcattgcc aagggggcgg gacttgcccc atccctggaa 420ctatgaatgt
ctcagcccct tgagatcacc tgggcgtgga agaaagt 467138902DNAHomo
sapiensmisc_featureIncyte ID No 2989375 138cactgcactc cagtctaggt
gacagagaag gactcgtctc aaaaaataaa aataaataaa 60aaggaagcaa ggctaatcat
cagtatgtgc ttgttacaag agctatgatg aaggcactcc 120ttcgagttta
accaaatgag atcatctctg tcatgtgcct cacgcctcac agggactcca
180tgtgtgaaga ttcccccttc actcaccaga tcatctccat ggcaacagct
tgcagcctgc 240tcttggagtg ctttgttttg gcagcttctc tgctagtttg
tgtatggagt gaatggagga 300ggtaaatcca cagattaaga atatgctgtc
aggagtcagg cagccaaggt cagaagccag 360ctctgcttct cagtgctttc
tctttacaac acaggacttt gcaaggaaca tataattctg 420tgactagcgc
catttggaaa atgttgaaac tgaagtagag atgagagatc ttacgtctgc
480ctacccagtg agatacgagg aaggtcaagg gaaaaaaaat tccaagctct
tctttatctg 540ctataggaaa tgaacattca attttttgca tgcaacgaca
agaggtcaag gaccccagaa 600gccagcccgc tacttccaag ttgagagccc
ctggtcatac cctccagttg agctcagatt 660tgtcacaaat ttacccctct
cctttccttc cattccccat gacctgcaga gagagatgtc 720agataccttc
ctcttggcct cccatgggca tccataagaa acttacttga agcaagaagc
780ccagtatagg tgtctgggca gttggacatt tcctctagcc agatctgtcc
gaatagagcc 840atctgggtac atgacgcaga gggcatttga taaataactg
gaaaagtcaa taaatctttg 900tc 9021391332DNAHomo
sapiensmisc_featureIncyte ID No 3316764 139cgcagatgtg ccttcctggt
tggttgagat gctgatccta cagcactccc gctgtgcctc 60agcagtgagc tgggtgtaaa
ggcaggaggc ttgctggggt ctgacacttc cctgccctcc 120tccaggaggg
acacatctgg ggctctatga ggaggacagc tttcatcctg ggctctggac
180ttctctcatt tgtggccttc tggaactcag tgacatggca tcttcagaga
ttttggggtg 240cttctggcta cttttggcaa gcccagtggg agaggctgct
gactacattt gaagggaagg 300agtggatcct cttctttata ggtgccatcc
aagtgccttg tctcttcttc tggagcttca 360atgggcttct attggtggtt
gacacaacag gaaaacctaa cttcatctct cgctaccgaa 420ttcaggtcgg
caagaatgaa cctgtggatc ctgtgaaact gcgccagtct atccgcacag
480ttcttttcaa ccagtgcatg atatctttcc ccatggtggt cttcctctat
cccttcctca 540aatggtggag agacccctgc cgccgtgagc tacccacctt
ccactggttc ctcctggagc 600tggccatctt cacgctgatc gaggaagtct
tgttctacta ttcacaccgg ctccttcacc 660acccaacatt ctacaagaaa
atccacaaga aacaccatga gtggacagct cccattggcg 720tgatctctct
ctatgcccac cctatagagc atgcagtctc caacatgcta ccggtgatag
780tgggcccatt agtaatgggc tcccacttgt cctccatcac catgtggttt
tccttggccc 840tcatcatcac caccatctcc cactgtggct accaccttcc
cttcctgcct tcgcctgaat 900tccacgacta ccaccatctc aagttcaacc
agtgctatgg ggtgctgggt gtgctggacc 960acctccatgg gactgacacc
atgttcaagc agaccaaggc ctacgagaga catgtcctcc 1020tgctgggctt
caccccgctc tctgagagca tcccagactc cccaaagagg atggagtgag
1080agacagccta agtgtcatcc tggctgtccc tcagccatgg gatgcagaca
cggcttcctg 1140attgcaccta acaatttgcc tccttcggcc acacgcccta
atgatggcac caccagggta 1200gagggaaggt cggcttcccg gaaaagcagg
gccaaggatg aggctttctt caaactactg 1260cccttgatgt ccctcaatgg
gatcaggagt tagcttaaaa aaaaaaaaaa acaactgcgg 1320ccgcaagctt at
13321401252DNAHomo sapiensmisc_featureIncyte ID No 3359559
140gtgaggaagg tagctttagt gaaaacaggg tttggagttg aacctatacg
ggttcaaatt 60cgacttccgt ccaccaccga gacctgcgct ccctgaggga ctcgctttcc
catccgcgaa 120accaggacgg cgccgcctac accccgcggc gttcggggcg
ggctgaatgg gtcgctgagt 180aggggctaca cccacgccct tcgctccccg
cccccggcac ggagcgacgg ccacggcagt 240gtccccaagg caccgaaacc
gaggcggggg tctcggtccc tccgcgcaag gagggaggcg 300gaccgtacgt
ggcaggactc accgccccgc acgtggcagg actcaccgcc ccgcgccgtg
360ttctccgagc catggcgcca gcgctgtggc gggcctgcaa cggactcatg
gccgccttct 420tcgcgctggc ggccttggtg caggtaaatg acccagatgc
agaggtgtgg gtggtggtgt 480acacaatccc tgcagtactg accctgcttg
ttggacttaa ccctgaagtc acaggtaatg 540ttatttggaa aagtatctct
gcaatacaca tactcttttg tacggtgtgg gctgttggct 600tggcgtccta
cctcttgcat cgtacacaac agaacatctt acatgaggaa gaaggcaggg
660agctgtctgg tctggtgatt attacagcat ggattatcct gtgccacagt
tcctcaaaga 720atccagttgg tggaagaatt caattggcta ttgccattgt
aatcacactt ttcccattta 780tctcatgggt ctacatatat attaacaagg
aaatgcggtc ctcttggcca actcactgca 840agacagtaat ttaaataaat
tcaagaactt cgtttttaaa atgaatattt tcaatcaatt 900ttttataaac
attaggggaa caagccagga gtttatttca ggtaatttgg gctaatagtt
960ttaaaactcc aaataacttt ttaagggtgc atataattcg atgtaagatt
ggatgggaca 1020agtaagagat ggtctgatat tttccagacg actttctgca
gggtcttgtg tcataatgta 1080gtggaaaagg ctagagaata gaagtttaaa
aatacgagtt ctaacttaac tttgtaacta 1140tgtaatttgg gcaaatatat
aaacctcctg gtggatattt atctataaaa taggattaat 1200gccagagtgt
acttacttac acagtaacaa ggatcaatct agataatgta tg 1252141721DNAHomo
sapiensmisc_featureIncyte ID No 4289208 141ggagactgca ttccctgccc
tgaaggaatg tatttctaag gcaaataggc aacttggtac 60tatcttattc tgagtagaga
gtggagaaag tattttcaga ctgaagaaaa ctttgaaaag 120tcaggagcta
agctgctcgg agctcagtgc cgcagcatgg ctgtggtgga cgcgggaaac
180aacgggaaag ttcttgacag agtctgtgtc cgctcagtcc ctgcactttt
cctttccaaa 240tgcatctcgt tggatatgga atagatcgta gatgttgtag
actgagattt gggactatgt 300tgggaccgta caggtgaatg tgccacctcc
acaaatggct tctccgagtg agtcacgtca 360cctggtgcgt ggaggtggag
ctgcggctgg agtaaggctt gctgtgggac gccctcgtac 420tttgctcccc
ttgcgggtgg ttgccgaccc gagagcattg ggatcctccc ccgactggtg
480gctaagtttg tcctgtcccg ggttggctgg ggaaaggggg gttgtgggtt
cgggaaaaaa 540aagttccggg gaaattcctc ctggcaaaat tccggttggt
tcacattggg aacctggtta 600acctaaattt gggtaaaagg ggtccctaat
aattcgccct gggaaattcg tgggggggtt 660ccccaaggaa ccccctcgga
gtcccagggg ggagaaattt gaagagcccc tttcgaaatg 720g 7211421704DNAHomo
sapiensmisc_featureIncyte ID No 2454013 142cgcttcgcgc taacgcttgc
gatggttgaa ttcccctcct cacgccagcc taggagaaga 60agttcgtagt cccagaggtg
aggcaggagg cggcagtttc tggcgggtga gggcggagct 120gaagtgacag
cggaggcgga agcaacggtc ggtggggcgg agaagggggc tggccccagg
180aggaggagga aacccttccg agaaaacagc aacaagctga gctgctgtga
cagaggggaa 240caagatggcg gcgccgaagg ggagcctctg ggtgaggacc
caactggggc tcccgccgct 300gctgctgctg accatggcct tggccggagg
ttcggggacc gcttcggctg aagcatttga 360ctcggtcttg ggtgatacgg
cgtcttgcca ccgggcctgt cagttgacct accccttgca 420cacctaccct
aaggaagagg agttgtacgc atgtcagaga ggttgcaggc tgttttcaat
480ttgtcagttt gtggatgatg gaattgactt aaatcgaact aaattggaat
gtgaatctgc 540atgtacagaa gcatattccc aatctgatga gcaatatgct
tgccatcttg gttgccagaa 600tcagctgcca ttcgctgaac tgagacaaga
acaacttatg tccctgatgc caaaaatgca 660cctactcttt cctctaactc
tggtgaggtc attctggagt gacatgatgg actccgcaca 720gagcttcata
acctcttcat ggacttttta tcttcaagcc gatgacggaa aaatagttat
780attccagtct aagccagaaa tccagtacgc accacatttg gagcaggagc
ctacaaattt 840gagagaatca tctctaagca aaatgtccta tctgcaaatg
agaaattcac aagcgcacag 900gaattttctt gaagatggag aaagtgatgg
ctttttaaga tgcctctctc ttaactctgg 960gtggatttta actacaactc
ttgtcctctc ggtgatggta ttgctttgga tttgttgtgc 1020aactgttgct
acagctgtgg agcagtatgt tccctctgag aagctgagta tctatggtga
1080cttggagttt atgaatgaac aaaagctaaa cagatatcca gcttcttctc
ttgtggttgt 1140tagatctaaa actgaagatc atgaagaagc agggcctcta
cctacaaaag tgaatcttgc 1200tcattctgaa atttaagcat ttttctttta
aaagacaagt gtaatagaca tctaaaattc 1260cactcctcat agagctttta
aaatggtttc attggatata ggccttaaga aatcactata 1320aaatgcaaat
aaagttactc aaatctgtga agactgtatt tgctataact ttattggtat
1380tgtttttgta gtaatttaag aggtggatgt ttgggattgt attattattt
tactaatatc 1440tgtagctatt ttgttttttg ctttggttat tgtttttttc
ccttttctta gctatgagct 1500gatcattgct ccttctcacc tcctgccatg
atactgtcag ttaccttagt taacaagctg 1560aatatttagt agaaatgatg
cttctgctca ggaatggccc acaaatctgt aatttgaaat 1620ttagcaggaa
atgaccttta atgacactac attttcagga actgaaatca ttaaaatttt
1680atttgaataa ttaaaaaaaa aaaa 1704143964DNAHomo
sapiensmisc_featureIncyte ID No 2454048 143cagacagcgg cgggcgcagg
acgtgcacta tggctcgggg ctcgctgcgc cggttgctgc 60ggctcctcgt gctggggctc
tggctggcgt tgctgcgctc cgtggccggg gagcaagcgc 120caggcaccgc
cccctgctcc cgcggcagct cctggagcgc ggacctggac aagtgcatgg
180actgcgcgtc ttgcagggcg cgaccgcaca gcgacttctg cctgggctgc
gctgcagcac 240ctcctgcccc cttccggctg ctttggccca tccttggggg
cgctctgagc ctgaccttcg 300tgctggggct gctttctggc tttttggtct
ggagacgatg ccgcaggaga gagaagttca 360ccacccccat agaggagacc
ggcggagagg gctgcccagc tgtggcgctg atccagtgac 420aatgtgcccc
ctgccagccg gggctcgccc actcatcatt cattcatcca ttctagagcc
480agtctctgcc tcccagacgc ggcgggagcc aagctcctcc aaccacaagg
ggggtggggg 540gcggtgaatc acctctgagg cctgggccca gggttcaggg
gaaccttcca aggtgtctgg 600ttgccctgcc tctggctcca gaacagaaag
ggagcctcac gctggctcac acaaaacagc 660tgacactgac taaggaactg
cagcatttgc acaggggagg ggggtgccct ccttcctaga 720ggccctgggg
gccaggctga cttggggggc agacttgaca ctaggcccca ctcactcaga
780tgtcctgaaa ttccaccacg ggggtcaccc tggggggtta gggacctatt
tttaacacta 840gggggctggc ccactaggag ggctggccct aagatacaga
cccccccaac tccccaaagc 900ggggaggaga tatttatttt ggggagagtt
tggaggggag ggagaattta ttaataaaag 960aatc 9641441564DNAHomo
sapiensmisc_featureIncyte ID No 2479282 144ggaattgtgg gagttgtgtc
tgccactcgg ctgccggagg ccgaaggtcc ctgactatgg 60ctccccagag cctgccttca
tctaggatgg ctcctctggg catgctgctt gggctgctga 120tggccgcctg
cttcaccttc tgcctcagtc atcagaacct gaaggagttt gccctgacca
180acccagagaa gagcagcacc aaagaaacag agagaaaaga aaccaaagcc
gaggaggagc 240tggatgccga agtcctggag gtgttccacc cgacgcatga
gtggcaggcc cttcagccag 300ggcaggctgt ccctgcagga tcccacgtac
ggctgaatct tcagactggg gaaagagagg 360caaaactcca atatgaggac
aagttccgaa ataatttgaa aggcaaaagg ctggatatca 420acaccaacac
ctacacatct caggatctca agagtgcact ggcaaaattc aaggaggggg
480cagagatgga gagttcaaag gaagacaagg caaggcaggc tgaggtaaag
cggctcttcc 540gccccattga ggaactgaag aaagactttg atgagctgaa
tgttgtcatt gagactgaca 600tgcagatcat ggtacggctg atcaacaagt
tcaatagttc cagctccagt ttggaagaga 660agattgctgc gctctttgat
cttgaatatt atgtccatca gatggacaat gcgcaggacc 720tgctttcctt
tggtggtctt caagtggtga tcaatgggct gaacagcaca gagcccctcg
780tgaaggagta tgctgcgttt gtgctgggcg ctgccttttc cagcaacccc
aaggtccagg 840tggaggccat cgaaggggga gccctgcaga agctgctggt
catcctggcc acggagcagc 900cgctcactgc aaagaagaag gtcctgtttg
cactgtgctc cctgctgcgc cacttcccct 960atgcccagcg gcagttcctg
aagctcgggg ggctgcaggt cctgaggacc ctggtgcagg 1020agaagggcac
ggaggtgctc gccgtgcgcg tggtcacact gctctacgac ctggtcacgg
1080agaagatgtt cgccgaggag gaggctgagc tgacccagga gatgtcccca
gagaagctgc 1140agcagtatcg ccaggtacac ctcctgccag gcctgtggga
acagggctgg tgcgagatca 1200cggcccacct cctggcgctg cccgagcatg
atgcccgtga gaaggtgctg cagacactgg 1260gcgtcctcct gaccacctgc
cgggaccgct accgtcagga cccccagctc ggcaggacac 1320tggccagcct
gcaggctgag taccaggtgc tggccagcct ggagctgcag gatggtgagg
1380acgagggcta cttccaggag ctgctgggct ctgtcaacag cttgctgaag
gagctgagat 1440gaggccccac accaggactg gactgggatg ccgctagtga
ggctgagggg tgccagcgtg 1500ggtgggcttc tcaggcagga ggacatcttg
gcagtgctgg cttggccatt aaatggaaac 1560ctgg 15641451385DNAHomo
sapiensmisc_featureIncyte ID No 2483432 145gtccgcccgc cgctgcgtcc
cggagtgcaa gtgagcttct cggctgcccc gcgggccggg 60gtgcggagcc gacatgcgcc
cgcttctcgg cctccttctg gtcttcgccg gctgcacctt 120cgccttgtac
ttgctgtcga cgcgactgcc ccgcgggcgg agactgggct ccaccgagga
180ggctggaggc aggtcgctgt ggttcccctc cgacctggca gagctgcggg
agctctctga 240ggtccttcga gagtaccgga aggagcacca ggcctacgtg
ttcctgctct tctgcggcgc 300ctacctctac aaacagggct ttgccatccc
cggctccagc ttcctgaatg ttttagctgg 360tgccttgttt gggccatggc
tggggcttct gctgtgctgt gtgttgacct cggtgggtgc 420cacatgctgc
tacctgctct ccagtatttt tggcaaacag ttggtggtgt cctactttcc
480tgataaagtg gccctgctgc agagaaaggt ggaggagaac agaaacagct
tgtttttttt 540cttattgttt ttgagacttt tccccatgac accaaactgg
ttcttgaacc tctcggcccc 600aattctgaac attcccatcg tgcagttctt
cttctcagtt cttatcggtt tgatcccata 660taatttcatc tgtgtgcaga
cagggtccat cctgtcaacc ctaacctctc tggatgctct 720tttctcctgg
gacactgtct ttaagctgtt ggccattgcc atggtggcat taattcctgg
780aaccctcatt aaaaaattta gtcagaaaca tctgcaattg aatgaaacaa
gtactgctaa 840tcatatacac agtagaaaag acacatgatc tggattttct
gtttgccaca tccctggact 900cagttgctta tttgtgtaat ggatgtggtc
ctctaaagcc cctcattgtt tttgattgcc 960ttctataggt gatgtggaca
ctgtgcatca atgtgcagtg tcttttcaga aaggacactc 1020tgctcttgaa
ggtgtattac atcaggtttt caaaccagcc ctggtgtagc agacactgca
1080acagatgcct cctagaaaat gctgtttgtg gccgggcgcg gtggctcacg
cctgtaatcc 1140cagcactttg ggaggccgag gccggtgatt cacaaggtca
ggagttcaag accagcctgg 1200ccaagatggt gaaatcctgt ctctaataaa
aatacaaaaa ttagccaggc gtggtggcag 1260gcacctgtaa tcccagctac
tcgggaggct gaggcaggag aattgcttga accaaggtgg 1320cagaggttgc
agtaagccaa gatcacacca ctgcactcca gcctgggtga tagagtgaga 1380ccaca
13851462031DNAHomo sapiensmisc_featureIncyte ID No 2493824
146tgggcggggg cccacggcgg ccactcactg agccccacgg gccgcagcgg
cagtgacgta 60gggttggcgc acggatccgt tgcggctgca gctctgcagt cgggccgttc
cttcgccgcc 120gccaggggta gcggtgtagc tgcgcagcgt cgcgcgcgct
accgcaccca ggttcggccc 180ataggcgtct ggcagcccgg cgccatcttc
atcgagcgcc atggccgcag cctgcgggcc 240gggagcggcc gggtactgct
tgctcctcgg cttgcatttg tttctgctga ccgcgggccc 300tgccctgggc
tggaacgacc ctgacagaat gttgctgcgg gatgtaaaag ctcttaccct
360ccactatgac cgctatacca cctcccgcag gctggatccc atcccacagt
tgaaatgtgt 420tggaggcaca gctggttgtg attcttatac cccaaaagtc
atacagtgtc agaacaaagg 480ctgggatggg tatgatgtac agtgggaatg
taagacggac ttagatattg catacaaatt 540tggaaaaact gtggtgagct
gtgaaggcta tgagtcctct gaagaccagt atgtactaag 600aggttcttgt
ggcttggagt ataatttaga ttatacagaa cttggcctgc agaaactgaa
660ggagtctgga aagcagcacg gctttgcctc tttctctgat tattattata
agtggtcctc 720ggcggattcc tgtaacatga gtggattgat taccatcgtg
gtactccttg ggatcgcctt 780tgtagtctat aagctgttcc tgagtgacgg
gcagtattct cctccaccgt actctgagta 840tcctccattt tcccaccgtt
accagagatt caccaactca gcaggacctc ctcccccagg 900ctttaagtct
gagttcacag gaccacagaa tactggccat ggtgcaactt ctggttttgg
960cagtgctttt acaggacaac aaggatatga aaattcagga ccagggttct
ggacaggctt 1020gggaactggt ggaatactag gatatttgtt tggcagcaat
agagcggcaa cacccttctc 1080agactcgtgg tactacccgt cctatcctcc
ctcctaccct ggcacgtgga atagggctta 1140ctcacccctt catggaggct
cgggcagcta ttcggtatgt tcaaactcag acacgaaaac 1200cagaactgca
tcaggatatg gtggtaccag gagacgataa agtagaaagt tggagtcaaa
1260cactggatgc agaaattttg gatttttcat cactttctct ttagaaaaaa
agtactacct 1320gttaacaatt gggaaaaggg gatattcaaa agttctgtgg
tgttatgtcc agtgtagctt 1380tttgtattct attatttgag gctaaaagtt
gatgtgtgac aaaatactta tgtgttgtat 1440gtcagtgtaa catgcagatg
tatattgcag tttttgaaag tgatcattac tgtggaatgc 1500taaaaataca
ttaatttcta aaacctgtga tgccctaaga agcattaaga atgaaggtgt
1560tgtactaata gaaactaagt acagaaaatt tcagttttag gtggttgtag
ctgatgagtt 1620attacctcat agagactata atattctatt tggtattata
ttatttgatg tttgctgttc 1680ttcaaacatt taaatcaagc tttggactaa
ttatgctaat ttgtgagttc tgatcacttt 1740tgagctctga agctttgaat
cattcagtgg tggagatggc cttctggtaa ctgaatatta 1800ccttctgtag
gaaaaggtgg aaaataagca tctagaaggt tgttgtgaat gactctgtgc
1860tggcaaaaat gcttgaaacc tctatatttc tttcgttcat aagaggtaaa
ggtcaaattt 1920ttcaacaaaa gtcttttaat aacaaaagca tgcagttctc
tgtgaaatct caaatattgt 1980tgtaatagtc tgtttcaatc ttaaaaagaa
tcaataaaaa caaaaaaaaa a 20311471790DNAHomo
sapiensmisc_featureIncyte ID No 2555823 147gcgggaggac cggctgaccc
tggatggtga ggccgggtgc ccgcctgtgc ctggggagtg 60tggggagggg gctgtgcctg
gtgctccccc tgctttgtct cggtgcaggt ttcctcttcc 120tgaacacgct
cttcatccag cgcggccggc acgagaccac ctggaccatc ctgcggcgct
180tcggctacag cgatgccctg gagctgactg cggactatct ctcccctctg
atccacgtgc 240cccccggctg cagcacggag ctcaaccacc ttggctacca
gtttgtgcag agagtgtttg 300agaagcacga ccaggaccgc gacggcgccc
tctcgcccgt ggagctgcaa agccttttca 360gtgtgttccc agcagcgccc
tggggccccg agctcccacg cacagtccgc acagaggccg 420gccggttgcc
cctgcacgga tacctctgcc agtggaccct ggtgacctac ctggacgtcc
480ggagctgcct tggacaccta ggctacctgg gctaccccac cctctgtgag
caggaccagg 540cccatgccat cacagtcact cgtgagaaga ggctggacca
ggagaaggga cagacgcagc 600ggagcgtcct cctgtgcaag gtggtagggg
cccgtggagt gggcaagtct gccttcctgc 660aggcctttct cggccgcggc
ctggggcacc aggacacgag ggagcagcct cccggctacg 720ccatcgacac
ggtgcaggtc aatggacagg agaagtactt gatcctctgt gaggtgggca
780cagatggtct gctggccaca tcgctggacg ccacctgtga cgttgcctgc
ttgatgtttg 840atggcagtga cccaaagtcc tttgcacatt gtgccagcgt
ctacaagcac cattacatgg 900acgggcagac cccctgcctc tttgtctcct
ccaaggccga cctgcccgaa ggtgtcgcgg 960tgtctggccc atcaccggcc
gagttttgcc gcaagcaccg gctacccgct cccgtgccgt 1020tctcctgtgc
tggcccagcc gagcccagca ccaccatctt cacccagctc gccaccatgg
1080ccgccttccc acatttggtc cacgcagagc tgcatccctc ttccttctgg
ctccgggggc 1140tgctgggggt tgtcggggcc gccgtggccg cagtcctcag
cttctcactc tacagggtcc 1200tggtgaagag ccagtgaggc ccctggtacc
caagccccct cccctgacct gggtgtgcct 1260cgctgctggg gctctgcagg
ggcagcacag ctggggtgca ggccaggctg ccactccggg 1320aacgcctttg
cgccgggact ttttgtttct gaaggcagtc gatctgcagc ggggccttat
1380gctgccatgc actgccctgg ctcctgccgg acccccaggg tgggccgtgg
caggtggctg 1440agcaggagct cccaagtgcc ggccaccgct gtcagggatt
gcccacccct gggcatcatg 1500tgtgtggggc cggggagcac
aggtgtggga gctggtgacc ccagacccag aattctcagg 1560gctctacccc
cctttcctgg tcctaggtgg ccagtgggta tgaggagggc tggaaggcag
1620agctttgggc caaaagcagg cgttgggggg tcccccctca agtttggagc
cgtttccgtg 1680gttgtagcag aggaccggag gttgggttcc tgattaaact
tcactgtgtg ttttctatct 1740cggatcccag tctctgaaga caacttgctt
tgattcaacc taaaaaaaaa 17901481979DNAHomo sapiensmisc_featureIncyte
ID No 2598242 148ctactcctca ctggccggga caactggtct tatcacggag
gctggggcca ggcagccctt 60cggttcgggt gggcccatgg accccagtcc aacgccgagg
gaataggacc atccaaaagc 120ggaaccttcg cctcagaaaa agggtgcggg
acccctcctc accgtgcggt cacgcgtgga 180ccctgccagc agccaggcca
tggagctctc tgatgtcacc ctcattgagg gtgtgggtaa 240tgaggtgatg
gtggtggcag gtgtggtggt gctgattcta gccttggtcc tagcttggct
300ctctacctac gtagcagaca gcggtagcaa ccagctcctg ggcgctattg
tgtcagcagg 360cgacacatcc gtcctccacc tggggcatgt ggaccacctg
gtggcaggcc aaggcaaccc 420cgagccaact gaactccccc atccatcaga
gggtaatgat gagaaggctg aagaggcggg 480tgaaggtcgg ggagactcca
ctggggaggc tggagctggg ggtggtgttg agcccagcct 540tgagcatctc
cttgacatcc aaggcctgcc caaaagacaa gcaggtgcag gcagcagcag
600tccagaggcc cccctgagat ctgaggatag cacctgcctc cctcccagcc
ctggcctcat 660cactgtgcgg ctcaaattcc tcaatgatac cgaggagctg
gctgtggcta ggccagagga 720taccgtgggt gccctgaaga gcaaatactt
ccctggacaa gaaagccaga tgaaactgat 780ctaccagggc cgcctgctac
aagacccagc ccgcacactg cgttctctga acattaccga 840caactgtgtg
attcactgcc accgctcacc cccagggtca gctgttccag gcccctcagc
900ctccttggcc ccctcggcca ctgagccacc cagccttggt gtcaatgtgg
gcagcctcat 960ggtgcctgtc tttgtggtgc tgttgggtgt ggtctggtac
ttccgaatca attaccgcca 1020attcttcaca gcacctgcca ctgtctccct
ggtgggagtc accgtcttct tcagcttcct 1080agtatttggg atgtatggac
gataaggaca taggaagaaa atgaaaggca tggtctttct 1140cctttatggc
ctccccactt ttcctggcca gagctgggcc caagggccgg ggagggaggg
1200gtggaaagga tgtgatggaa atctcctcca taggacacag gaggcaagta
tgcggcctcc 1260ccttctcatc cacaggagta cagatgtccc tcccgtgcga
gcacaactca ggtagaaatg 1320aggatgtcat cttccttcac ttttagggtc
ctctgaagga gttcaaagct gctggccaag 1380ctcagtgggg agcctgggct
ctgagattcc ctcccacctg tggttctgac tcttcccagt 1440gtcctgcatg
tctgccccca gcacccaggg ctgcctgcaa gggcagctca gcatggcccc
1500agcacaactc cgtagggagc ctggagtatc cttccatttc tcagccaaat
actcatcttt 1560tgagactgaa atcacactgg cgggaatgaa gattgtgcca
gccttctctt atgggcacct 1620agccgccttc accttcttcc tctacccctt
agcaggaata gggtgtcctc ccttctttca 1680aagcactttg cttgcatttt
attttatttt tttaagagtc cttcatagag ctcagtcagg 1740aaggggatgg
ggcaccaagc caagccccca gcattgggag cggccaggcc acagctgctg
1800ctcccgtagt cctcaggctg taagcaagag acagcactgg cccttggcca
gcgtcctacc 1860ctgcccaact ccaaggactg ggtatggatt gctgggccct
aggctcttgc ttctggggct 1920attggagggt cagtgtctgt gactgaataa
agttccattt tgtggtcaaa aaaaaaaaa 19791491810DNAHomo
sapiensmisc_featureIncyte ID No 2634120 149ccccctgccc gcctctccgc
acaatacttg aacattcatc tgtactgaag tgttacttga 60accgggggaa tctcggacct
gggggagccg gggtgtgagg ggactggacc agcttggact 120gagacctgag
accgggccgg tgggcgccca tttgggactg cgccaccccc aggcttgttc
180ttgttttact gtattgagcg gcggcacccg ccggacccgc attatggctg
ggggcgccag 240ccaagaatgg ggaccatggg actcctccag cctggctctt
cccactcttt catcgtcatg 300gaaacttgta tcccatttgc ccagggaact
gccactcctg gttgccatgg aaatagcagc 360caacggacac ctcccgatgc
cagtgctaag gctggaaatg gccccctctt agttgccatg 420ggaacctagt
aacagactct gctggccctc cttccctgcc ccttcctcga gcgcggggtg
480gggcttcggg accccgggga tgagccgggc caggtcccgc ccctccgcgc
aggcctccgg 540ggggccgggg cttaccatgt aggggagggg agatctatcc
acatacctca ggtggccatg 600gtggaggtgc agctggagag tgaccacgag
tacccaccag gcctgctggt ggccttcagt 660gcctgcacca ccgtgctggt
ggctgtgcac ctctttgcac tcatggtctc cacgtgtctg 720ctgccccaca
ttgaagctgt gagcaacatc cacaacctca actctgtcca ccagtcgcca
780caccagagac tgcaccgcta cgtggagctg gcctggggct tctccactgc
cctgggcacc 840tttctcttcc ttgctgaagt tgtcctggtt ggttgggtca
agtttgtgcc cattggggct 900cccttggaca caccgacccc catggtgccc
acatcccggg tgcccgggac tctggcacca 960gtggctacct cccttagtcc
agcttccaat ctcccacggt cctctgcgtc tgcagcaccg 1020tcccaggctg
agccagcctg cccaccccgg caagcctgtg gtggtggtgg ggcccatggg
1080ccaggctggc aagcagccat ggcctccaca gccatcatgg tacccgtggg
gctcgtgttt 1140gtggcctttg ccctgcattt ctaccgctcc ttggtggcac
acaagacaga ccgctacaag 1200caggaactag aggaactgaa tcgcctgcag
ggggagctgc aggctgtgtg agactggtgt 1260tagccaccgc tcactgcaag
cactgcctcc ctccggggtc tgtaagaggc cgcaggggcc 1320tacagacctc
atccccccat cccctggctg gagccacttc cagtggccac tctcaggcag
1380agttcagatt cctgcccgca ggtcctctgg gctgggcctt ggggcagctc
ccacattccc 1440agggattttc cccatcagtc tgtcccttgg gttttgcaag
ctactctgca cctgggctgg 1500cctcagttga aggatcatgc agtagataga
ggggaggcag ggagagcttg tgggaccttc 1560agtgctgact ttagccacca
tttccattcc tatacaggat gtgaaggtca gaaggcagcc 1620aattgttggt
ttaatttttt ttttttttga gacagtctgt ttcccaggct ggagtgtagt
1680gatacagtca cagctcactg tagcctcgac cttccaggct caaaagatgc
tcccaccaca 1740gcctcccagg tagtgagtag ctggtactac aggtgtgtgc
tgccacaccc gactaatttt 1800tttgtagaga 1810150535DNAHomo
sapiensmisc_featureIncyte ID No 2765411 150gaggaaccag aaatttgtcc
ttgaataatg tttcccgtgt tgggctggat cttgatagca 60gttgttatca tcattcttct
gatttttaca tctgtcaccc gatgcctatc tccagttagt 120tttctgcagc
tgaaattctg gaaaatctat ttggaacagg agcagcagat ccttaaaagt
180aaagccacag agcatgcaac tgaattggca aaagagaata ttaaatgttt
ctttgagggc 240tcgcatccaa aagaatataa cactccaagc atgaaagagt
ggcagcaaat ttcatcactg 300tatactttca atccgaaggg ccagtactac
agcatgttgc acaaatatgt caacagaaaa 360gagaagactc acagtatcag
gtctactgaa ggagatacgg tgattcctgt tcttggcttt 420gtagattcat
ctggtataaa cagcactcct gagttatgac cttttgaatg agtagaaaaa
480aaaattgttt tgaattattg ctttattaaa aaataaacat tggtaaaaaa aaaaa
535151891DNAHomo sapiensmisc_featureIncyte ID No 2769412
151gaaaagaatc cgaggcacag ataaagataa gttttactgt catgctgctt
ttaacataac 60agagcaacat cacctaggaa aaaagtttgt aggaggattt ttaatccata
tatttgtctt 120atggctagat aaagatttct ctgaaaaaaa gaagcatgtc
aggaatctct gggtgcccct 180ttttcctctg gggacttcta gcattgttgg
gcttggcttt ggttatatca ctgatcttca 240atatttccca ctatgtggaa
aagcaacgac aagataaaat gtacagctac tccagtgacc 300acaccagggt
tgatgagtat tatattgaag acacaccaat ttatggtaac ttagatgata
360tgatttcaga accaatggat gaaaattgct atgaacaaat gaaagcccga
ccagagaaat 420ctgtaaataa gatgcaggaa gccaccccat ctgcacaggc
aaccaatgaa acacagatgt 480gctacgcctc acttgatcac agcgttaagg
ggaagcgtag aaagcccagg aaacagaata 540ctcatttctc agacaaggat
ggagatgagc aactacatgc aatagatgcc agcgtttcta 600agaccacctt
agtagacagt ttctccccag aaagccaggc agtagaggaa aacattcatg
660atgatcccat cagactgttt ggattgatcc gtgctaagag agaacctata
aactagctgg 720accatgatct agttcaatga tttggctcct attgaagatg
gcttctaaga aaacaagatg 780cacagaggac acagaaggac ttggcagcag
ggtgatgacc tgatcatttg ttgatgggat 840ggtggcttac ctcttattca
cagcttacac ttatgcatgc caaatgtaag g 8911522311DNAHomo
sapiensmisc_featureIncyte ID No 2842779 152gggcgcggca ccgcagctgg
atggctgggg ccgcccggat cgccgccgcc gccgccgccg 60cacgtacgtg gcatgcctgg
atgtccctgc cctggctgtg gcatggcggg cccaaggctc 120ctcttcctca
ctgcccttgc cctggagctc ttgggaaggg ctgggggttc ccagccggcc
180ctccggagcc gggggactgc gacggcctgt cgcctggaca acaaggaaag
cgagtcctgg 240ggggctctgc tgagcggaga gcggctggac acctggatct
gctccctcct gggttccctc 300atggtggggc tcagtggggt cttcccgttg
cttgtcattc ccctagagat ggggaccatg 360ctgcgctcag aagctggggc
ctggcgcctg aagcagctgc tcagcttcgc cctgggggga 420ctcttgggca
atgtgtttct gcatctgctg cccgaagcct gggcctacac gtgcagcgcc
480agccctggtg gtgaggggca gagcctgcag cagcagcaac agctggggct
gtgggtcatt 540gctggcatcc tgaccttcct ggcgttggag aagatgttcc
tggacagcaa ggaggagggg 600accagccagg cccccaacaa agaccccact
gctgctgccg ccgcactcaa tggaggccac 660tgtctggccc agccggctgc
agagcccggc ctcggtgccg tggtccggag catcaaagtc 720agcggctacc
tcaacctgct ggccaacacc atcgataact tcacccacgg gctggctgtg
780gctgccagct tccttgtgag caagaagatc gggctcctga caaccatggc
catcctcctg 840catgagatcc cccatgaggt gggcgacttt gccatcctgc
tccgggccgg ctttgaccga 900tggagcgcag ccaagctgca actctcaaca
gcgctggggg gcctactggg cgctggcttc 960gccatctgta cccagtcccc
caagggagta gaggagacgg cagcctgggt cctgcccttc 1020acctctggcg
gctttctcta catcgccttg gtgaacgtgc tccctgacct cttggaagaa
1080gaggacccgt ggcgctccct gcagcagctg cttctgctct gtgcgggcat
cgtggtaatg 1140gtgctgttct cgctcttcgt ggattaactt tccctgatgc
cgacgcccct gccccctgca 1200gcaataagat gctcggattc actctgtgac
cgcatatgtg agaggcagag agggcgagtg 1260gctgcgagag agaatgagcc
tcccgccaga caggagggag gtgcgtgtgg atgtatgtgg 1320tgtgcacatg
tggccagagg tgtgtgcgcg agaccgacac tgtgatccct gtgctgggtc
1380cggggcccag tgtagcgcct gtccccagcc atgctgtggt tacctctcct
tgccgccctg 1440tcaccttcac ctcctggagt aagcagcgag gaagagcagc
actggtccca agcagaggcc 1500ttgccctgct gggaccccgg gagtgagagc
agcccaagga tcccagggtg cagggaactc 1560cagagctgcc cacctcccac
tgccccctca gcacacacac agtccccagg cggcctaggg 1620gccaaggctg
gggcggcttt ggtccctttt cctggccctt ccttccccac ttctaagcca
1680aagaaaggag aggcaggtgc tcctgtaccc cagccccact cagcactgac
agtccccagc 1740tcctagtagt gagctgggag gcgcttccta agaccctttc
ctcagggctg ccctgggagc 1800tcattcctgg ccaacacgcc ctggcagcac
cagcagctct tgccacctcc agctgccaaa 1860cagcagcctg ccgggcaggg
agcagcccca ggccagagag gcctcccggt ccagctcagg 1920gatgctcctg
ccagcacagg ggccagggac tcctggagca ggcacatagt gagcccgggc
1980agccctgccc agctcaggcc cctttccttc cccattgagg ttggggtagg
tgggggcggt 2040gagggctcca cgttgtcagc gctcaggaat gtgctccggc
agagtgctga agccataatc 2100cccaaccatt tcccttgtct gacgcccagg
tactcagctg gcccactcca cagccaggcc 2160tggccctgcc cttcaccgtg
gatgttttca gaagtggcca tcgagaggtc tggatggttt 2220tatagcaact
ttgctgtgat tccgtttgta tctgtaaata tttgttctat agataagata
2280caaataaata ttatccacat aaaaaaaaaa a 23111532169DNAHomo
sapiensmisc_featureIncyte ID No 2966260 153gctgcaggcg gcgacggcta
caccatgggc cggctgctgc gggccgcccg gctgccgccg 60ctgctttcgc cgctgctgct
tctgctggtt gggggagcgt tcctgggtgc ctgtgtggct 120gggtctgatg
agcctggccc agagggcctc acctccacct ccctgctaga cctcctgctg
180cccactggct tggagccact ggactcagag gagcctagtg agaccatggg
cctgggagct 240gggctgggag cccctggctc aggcttcccc agcgaagaga
atgaagagtc tcggattctg 300cagccaccac agtacttctg ggaagaggag
gaagagctga atgactcaag tctggacctg 360ggacccactg cagattatgt
ttttcctgac ttaactgaga aggcaggttc cattgaagac 420accagccagg
ctcaagagct gccaaacctc ccctctccct tgcccaagat gaatctggtt
480gagcctccct ggcatatgcc tcccagagag gaggaagaag aggaagagga
agaggaggag 540atggagaagg aagaggtaga gaaacaagat gtggaggaag
aggaggagct gctccctgtg 600aatggatccc aagaagaagc caagcctcag
gtccgtgact tttctctcac cagcagcagc 660cagaccccag gggccaccaa
aagcaggcat gaagactccg gggaccaggc ctcatcaggt 720gtggaggtgg
agagcagcat ggggcccagc ttgctgctgc cttcagtcac cccaactata
780gtgactccgg gggaccagga ctccaccagc caagaggcag aggccacagt
gctgccagct 840gcagggcttg gggtagagtt cgaggctcct caggaagcaa
gcgaggaagc cactgcagga 900gcagctggtt tgtctggcca gcacgaggag
gtgccggcct tgccttcatt ccctcaaacc 960acagctccca gtggggccga
gcacccagat gaagatcccc ttggctctag aacctcagcc 1020tcttccccac
tggcccctgg agacatggaa ctgacacctt cctctgctac cttgggacaa
1080gaagatctca accagcagct cctagaaggg caggcagctg aagctcaatc
caggataccc 1140tgggattcta cgcaggtgat ctgcaaggac tggagcaatc
tggctgggaa aaactacatc 1200attctgaaca tgacagagaa catagactgt
gaggtgttcc ggcagcaccg ggggccacag 1260ctcctggccc tggtggaaga
ggtgctgccc cgccatggca gtggccacca tggggcctgg 1320cacatctctc
tgagcaagcc cagcgagaag gagcagcacc ttctcatgac actggtgggc
1380gagcaggggg tggtgcccac tcaagatgtc ctttccatgc tgggtgacat
ccgcaggagc 1440ctggaggaga ttggcatcca gaactattcc acaaccagca
gctgccaggc gcgggccagc 1500caggtgcgca gcgactacgg cacgctcttc
gtggtgctgg tggtcattgg ggccatctgc 1560atcatcatca ttgcgcttgg
cctgctctac aactgctggc agcgccggct gcccaagctc 1620aagcacgtgt
cgcacggcga ggagctgcgc ttcgtggaga acggctgcca cgacaacccc
1680acgctggacg tggccagcga cagccagtcg gagatgcagg agaagcaccc
cagcctgaac 1740ggcggcgggg ccctcaacgg cccggggagc tggggggcgc
tcatgggggg caagcgggac 1800cccgaggact cggacgtgtt cgaggaggac
acgcacctgt gagcgcagcc gaggcgcagg 1860ccgagtgggc cgccaggacc
aagcgaggtg gaccccgaaa cggacggccc ggagccagca 1920caagccccga
gcctacccgg gccgcccccg cggcctggcc ctcggcgcgg gctccttccc
1980gcttcccccg acttcacacg gcggacttcg gaccaactcc ctcactcccg
cccgaggggc 2040aggcctcaaa gcccgccttg gccccgcttt cccgcccctg
aaccccggcc ccgcgggcgg 2100cgggcggcgc ttcctgcgcc ccgggactca
attaaacccg cccggagacc acgccgggcc 2160cagcgaaaa 21691541480DNAHomo
sapiensmisc_featureIncyte ID No 2993326 154ggatggggat ctttgtctgg
tccttaacca acaggtattt caccaactta ttagcccttt 60cttgtaaaat ggccacatct
cgcggtggtg caggcctccg gccccatggg atccgcctgt 120aattactgcc
acttctctca tccccattca gatgcttgga ctttcggttt cttttgcccc
180gggtcctggt tgtgggctcc aggctcaact gagccagata gtcatctgcc
agggcctgga 240cagcagcagc aacctgagtc tcaagggcac ttacgttggt
ctgagcagag gctatcttgg 300cctgggcccc ttggtcagca tttggagtct
ggctttcagt tgcccgagcc ttagtggcag 360ccttccgagc cctagaccct
ctcttgaccc gcagggtggc tgccagggct tggtttgtga 420ctatctgagt
ggcaagtggg gcctcagaga tctcagagac agaatttggg cccctgctgg
480cagccttctt gcccttggat tttttgggcc tgatagccac tactgaggcc
tcaatctgcc 540tggtagctgc gtcagtgaca tttagagcct ctatatgctc
agtgtcagta tttatgacct 600tagcaattgt cttcctggct ttggaggctt
tcttggttcg gatggaggct gctgtggtgt 660ggatactggc tgtctcattg
gtaatttggc cttgggtggt agctgtatgg gtggcagttg 720cagccagcga
gacctcggtg gcactagcta tggccttatt tgcagccttc ttagccttgg
780aagctttctt aggcttgata gaggttatca aggcttggga actggctgac
tcattggcaa 840ttggagcgtg aatattctgt gagatgactg gtagctttag
gacctgcaag ggtgacttca 900gctgtatagt gccaccctca tggccagttg
gggattggga gccttgggct gccttggcag 960taactctctt catcttgttg
gctttcttag gctgagcagt gactgaagaa gcctgagtat 1020tggttacctc
agtggtaggt aaagcctggc tgatctgggt aatgactggc aggtttaaag
1080cctgccaagt tattttgggc ttgttggtgg caatctcatt ggcagctggg
actggagggg 1140cagcaggtgc agccttagta atagtcttta taggggcctt
cttggtcttg cttttcttag 1200gccggttgac aactggtggg tccatggcca
gggagtcctt ggttgccgcc aacagggtat 1260gcatcagcag gacactgtcc
tcttctgtgg tctcagtctg tatatctgga gggaagggaa 1320gccccaggct
ccccggggga ggcagagggc cctgaaatag aggcacccta tatccgtagt
1380catttctcct atccatcttt ctgggaggcc gagtaacagg tgagcctcgt
cttcttgaat 1440ccagaaggcg tctgctctct ccaagtctgc tctctccaag
14801551222DNAHomo sapiensmisc_featureIncyte ID No 3001124
155agaaatatca tatggttact ttggtatctg acacagccat gacaccaatt
gctagtgtag 60acacaatagc tgtgtgtctt tttgcaggag cctggggagg ggccatggtg
ccaatgcact 120tactggggag actggagaag ccgcttctcc tcctgtgctg
cgcctccttc ctactggggc 180tggctttgct gggcataaag acggacatca
cccccgttgc ttatttcttt ctcacattgg 240gtggcttctt cttgtttgcc
tatctcctgg tccggtttct ggaatggggg cttcggtccc 300agctccaatc
aatgcagact gagagcccag ggccctcagg caatgcacgg gacaatgaag
360cctttgaagt gccagtctat gaagaggccg tggtgggact agaatcccag
tgccgccccc 420aagagttgga ccaaccaccc ccctacagca ctgttgtgat
acccccagca cctgaggagg 480aacaacctag ccatccagag gggtccagga
gagccaaact ggaacagagg cgaatggcct 540cagaggggtc catggcccag
gaaggaagcc ctggaagagc tccaatcaac cttcggcttc 600ggggaccacg
ggctgtgtcc actgctcctg atctgcagag cttggcggca gtccccacat
660tagagcctct gactccaccc cctgcctatg atgtctgctt tggtcaccct
gatgatgata 720gtgtttttta tgaggacaac tgggcacccc cttaaatgac
tctcccaaga tttctcttct 780ctccacacca gacctcgttc atttgactaa
cattttccag cgcctactat gtgtcagaaa 840caagtgtttc tgcctggaca
tcataaatgg ggacttggac cctgaggaga gtcaggccac 900ggtaagccct
tcccagctga gatatgggtg gcataatttg agtcttctgg caacatttgg
960tgacctaccc catatccaat atttccagcg ttagattgag gatgaggtag
ggaggtgatc 1020cagagaaggc ggagaaggaa gaagtaacct ctgagtggcg
gctattgctt ctgttccagg 1080tgctgttcga gctgttagaa cccttaggct
tgacagcttt gtgagttatt attgaaaaat 1140gaggattcca agagtcagag
gagtttgata atgtgcacga gggcacactg ctagtaaata 1200acattaaaat
aactcgaatg ac 12221561983DNAHomo sapiensmisc_featureIncyte ID No
3120070 156ggaaccgcct ccccgcggcc tcttcgcttt tgtggcggcg cccgcgctcg
caggccactc 60tctgctgtcg cccgtcccgc gcgctcctcc gacccgctcc gctccgctcc
gctcggcccc 120gcgccgcccg tcaacatgat ccgctgcggc ctggcctgcg
agcgctgccg ctggatcctg 180cccctgctcc tactcagcgc catcgccttc
gacatcatcg cgctggccgg ccgcggctgg 240ttgcagtcta gcgaccacgg
ccagacgtcc tcgctgtggt ggaaatgctc ccaagagggc 300ggcggcagcg
ggtcctacga ggagggctgt cagagcctca tggagtacgc gtggggtaga
360gcagcggctg ccatgctctt ctgtggcttc atcatcctgg tgatctgttt
catcctctcc 420ttcttcgccc tctgtggacc ccagatgctt gtcttcctga
gagtgattgg aggtctcctt 480gccttggctg ctgtgttcca gatcatctcc
ctggtaattt accccgtgaa gtacacccag 540accttcaccc ttcatgccaa
ccctgctgtc acttacatct ataactgggc ctacggcttt 600gggtgggcag
ccacgattat cctgattggc tgtgccttct tcttctgctg cctccccaac
660tacgaagatg accttctggg caatgccaag cccaggtact tctacacatc
tgcctaactt 720gggaatgaat gtgggagaaa atcgctgctg ctgagatgga
ctccagaaga agaaactgtt 780tctccaggcg actttgaacc cattttttgg
cagtgttcat attattaaac tagtcaaaaa 840tgctaaaata atttgggaga
aaatattttt taagtagtgt tatagtttca tgtttatctt 900ttattatgtt
ttgtgaagtt gtgtcttttc actaattacc tatactatgc caatatttcc
960ttatatctat ccataacatt tatactacat ttgtaagaga atatgcacgt
gaaacttaac 1020actttataag gtaaaaatga ggtttccaag atttaataat
ctgatcaagt tcttgttatt 1080tccaaataga atggactcgg tctgttaagg
gctaaggaga agaggaagat aaggttaaaa 1140gttgttaatg accaaacatt
ctaaaagaaa tgcaaaaaaa aagtttattt tcaagccttc 1200gaactattta
aggaaagcaa aatcatttcc taaatgcata tcatttgtga gaatttctca
1260ttaatatcct gaatcattca tttcagctaa ggcttcatgt tgactcgata
tgtcatctag 1320gaaagtacta tttcatggtc caaacctgtt gccatagttg
gtaaggcttt cctttaagtg 1380tgaaatattt agatgaaatt ttctctttta
aagttcttta tagggttagg gtgtgggaaa 1440atgctatatt aataaatctg
tagtgttttg tgtttatatg ttcagaacca gagtagactg 1500gattgaaaga
tggactgggt ctaatttatc atgactgata gatctggtta agttgtgtag
1560taaagcatta gggtcattcc tgtcacaaaa gtgccactaa aacagcctca
ggagaataaa 1620tgacttgctt ttctaaatct
caggtttatc tgggctctat catatagaca ggcttctgat 1680agtttgcaac
tgtaagcaga aacctacata tagttaaaat cctggtcttt cttggtaaac
1740agattttaaa tgtctgatat aaaacatgcc acaggagaat tcggggattt
gagtttctct 1800gaatagcata tatatgatgc atcggatagg tcattatgat
tttttaccat ttcgacttac 1860ataatgaaaa ccaattcatt ttaaatatca
gattattatt ttgtaagttg tggaaaaagc 1920taattgtagt tttcattatg
aagttttccc aataaaccag gtattctaaa cttgaaaaaa 1980aaa
19831571835DNAHomo sapiensmisc_featureIncyte ID No 3133035
157accaggctgt gtaagagctg ctggagtagg cacccattta aagaaaaaat
gaagaagcag 60caataaagaa gttgtaatcg ttacctagac aaacagagaa ctggttttga
cagtgtttct 120agagtgcttt ttattatttt cctgacagtt gtgttccacc
atgattactt tctccttcag 180cgaataggct aaatgaatat gaaacagaaa
agcgtgtatc agcaaaccaa agcacttctg 240tgcaagaatt ttcttaagaa
atggaggatg aaaagagaga gcttattgga atggggcctc 300tcaatacttc
taggactgtg tattgctctg ttttccagtt ccatgagaaa tgtccagttt
360cctggaatgg ctcctcagaa tctgggaagg gtagataaat ttaatagctc
ttctttaatg 420gttgtgtata caccaatatc taatttaacc cagcagataa
tgaataaaac agcacttgct 480cctcttttga aaggaacaag tgtcattggg
gcacaaataa tacacacatg gacgaaatac 540ttctggaaaa tttacatatg
ctatgggaat catctttaat gaaactttct cttataagtt 600aatatttttc
cagggatata acagtccact ttggaaagaa gatttctcag gtgactttcc
660atatcaaata tcattatgga atttttcatg ttttcaacat aaagaacaga
ggaggttggg 720caacagagat gctttcaatg acacatgaga aaacagggaa
agcccatttc attgctgaac 780ttatttcaag gtcaatcgta tgttcctact
acaggatgac tgcaaaaatt gtagagtcat 840ccaacatata tgtgttgagc
atgcagatgc atgtgtcaaa ggacacatga gtaacccaag 900actgacaggc
cccagcctca ggtgagattc caggttagca gcaaagacag acattgaaca
960attaatgaca agtacaagaa aaagtgtttc atgggcactt agaccagggg
ttcctaatag 1020tgggacctag agaagtccta cctggggaaa tgatgtttaa
agggagacca gaatgaatag 1080caggtgtgag gtgctagaag cattgtgttt
cagatagaag aaaggtaatt gtgaagaccc 1140tgaggtgaga aaggacatct
gttcctagat ctggaagaag agcagtatag ctgaacaagg 1200aacatgaaaa
ggaatgtaat gggagagtga agctgaagtc actcaagtgc tacctcctgt
1260ggcatcttgt aaacctaggc aaggaatagc cactgagtca ctttaatcac
ggcaaaagtg 1320taattcggtt tccaaaatta ggggaacact ccagatatag
cccggggaat agattgccaa 1380gaggctatgg agaatgtcaa gaaacaagga
gtccattatg gctggagcag agtgtttgct 1440ttcatctcct ttttattttc
taagactttc taagcatgct gtggtctgca agaataaaat 1500tgctttatta
aaaactttca tttatttgct tcctttttct atgtagttaa aagtctactg
1560gtgggccagc catggtggct cacacctgta atcccagcac tttgagaggc
cgaggtgcac 1620ggatcacctg aggtcaggag ttcgagacca gcctggccaa
catggtgaaa gcctgtctct 1680actaaaaata caaaaattag ctagacaacg
tggcctgtgc ctataatccc agctttggga 1740ggctgaggta ggagaatcac
ttgaacccag gaggtggagg ttgcagtgag ctgagatcgc 1800accactgcac
tccagcatgg gcaacagagt gagat 1835158819DNAHomo
sapiensmisc_featureIncyte ID No 3436879 158cacgactcac tatagggaat
ttggccctcg aggcaagaat tcggcacgag gtcgacaccc 60tcatcctgaa aggtattgcg
cacgatgcac ggccatcaag taccactttt ctcagcccat 120ccgcttgcga
aacattcctt ttaatttaac caagaccata cagcaagatg agtggcacct
180gcttcattta agaagaatca ctgctggctt cctcggcatg gccgtagccg
tccttctctg 240cggctgcatt gtggccacag tcagtttctt ctgggaggag
agcttgaccc agcacgtggc 300tggactcctg ttcctcatga cagggatatt
ttgcaccatt tccctctgta cttatgccgc 360cagtatctcg tatgatttga
accggctccc aaagctaatt tatagcctgc ctgctgatgt 420ggaacatggt
tacagctggt ccatcttttg cgcctggtgc agtttaggct ttattgtggc
480agctggaggt ctctgcatcg cttatccgtt tattagccgg accaagattg
cacagctaaa 540gtctggcaga gactccacgg tatgactgtc ctcactgggc
ctgtccacag tgcgagcgac 600tcctgagggg aacagcgcgg agttcaggag
tccaagcaca aagcggtctt ttacattcca 660acctgttgcc tgccagccct
ttctggatta ctgatagaaa atcatgcaaa acctcccaac 720ctttctaagg
acaagactac tgtggattca agtgctttaa tgactattta tgcgttgact
780gtgagaatag ggagccatgc catgggacat ttctaggtg 819
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