U.S. patent application number 13/494015 was filed with the patent office on 2012-10-04 for porous materials for solid phase extraction and chromatography and processes for preparation and use thereof.
This patent application is currently assigned to Waters Technologies Corporation. Invention is credited to Darryl W. Brousmiche, Claude R. Mallet, John E. O'Gara.
Application Number | 20120248033 13/494015 |
Document ID | / |
Family ID | 22215980 |
Filed Date | 2012-10-04 |
United States Patent
Application |
20120248033 |
Kind Code |
A1 |
Mallet; Claude R. ; et
al. |
October 4, 2012 |
POROUS MATERIALS FOR SOLID PHASE EXTRACTION AND CHROMATOGRAPHY AND
PROCESSES FOR PREPARATION AND USE THEREOF
Abstract
Embodiments of the present invention are directed to porous
materials for use in solid phase extractions and chromatography.
The materials feature at least one hydrophobic component, at least
one hydrophilic component and at least one ion-exchange functional
group. The materials exhibit superior wetting and ion-exchange
performance.
Inventors: |
Mallet; Claude R.;
(Attleboro, MA) ; O'Gara; John E.; (Ashland,
MA) ; Brousmiche; Darryl W.; (Grafton, MA) |
Assignee: |
Waters Technologies
Corporation
Milford
MA
|
Family ID: |
22215980 |
Appl. No.: |
13/494015 |
Filed: |
June 11, 2012 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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12790795 |
May 28, 2010 |
8197692 |
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13494015 |
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10782397 |
Feb 18, 2004 |
7731844 |
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12790795 |
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10169546 |
Jan 16, 2003 |
7232520 |
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PCT/US99/13241 |
Jun 10, 1999 |
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10782397 |
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60089153 |
Jun 12, 1998 |
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Current U.S.
Class: |
210/502.1 ;
521/38 |
Current CPC
Class: |
B01J 20/28042 20130101;
B01J 39/20 20130101; B01J 39/26 20130101; B01J 20/26 20130101; B01J
2220/82 20130101; C08F 8/24 20130101; B01J 20/285 20130101; G01N
30/02 20130101; B01J 2220/54 20130101; C08F 2800/20 20130101; G01N
30/02 20130101; B01J 2220/62 20130101; C08F 8/36 20130101; B01J
41/20 20130101; B01J 20/264 20130101; C08F 8/24 20130101; B01J
2220/66 20130101; B01J 20/3285 20130101; B01J 20/261 20130101; C08F
8/32 20130101; C08F 212/36 20130101; B01D 15/361 20130101; C08F
212/36 20130101; C08F 212/36 20130101; C08F 8/24 20130101; C08F
8/36 20130101; C08F 8/32 20130101; B01J 2220/58 20130101 |
Class at
Publication: |
210/502.1 ;
521/38 |
International
Class: |
C08F 8/32 20060101
C08F008/32; B01D 15/08 20060101 B01D015/08 |
Claims
1. A porous material comprising a copolymer of at least one
hydrophobic monomer and at least one hydrophilic monomer, wherein
said copolymer further comprises at least one ion-exchange
functional moiety selected from the group consisting of an acyclic
secondary amine exclusive of polyethylenimine, a cyclic tertiary
amine, a substituted acyclic amine, and a substituted cyclic
amine.
2. The porous material of claim 1, wherein the porous material
comprises a porous particle that comprises said copolymer.
3. The porous material of claim 2, wherein said copolymer is
non-sulfonated.
4. The porous material of claim 2, wherein said substituted acyclic
amine or said substituted cyclic amine is substituted by an
electron withdrawing group.
5. The porous material of claim 2 wherein said hydrophobic monomer
is divinylbenzene or styrene.
6. The porous material of claim 2 wherein said hydrophilic monomer
is N-vinylpyrrolidone or N-vinyl acetamide.
7. The porous material of claim 2 wherein said copolymer is a
poly(divinylbenzene-co-N-vinylpyrrolidone).
8. The porous material of claim 2 wherein the hydrophobic monomer
is substituted by at least one haloalkyl group, and the
ion-exchange functional moiety is formed by reaction of the
haloalkyl group with an appropriate starting amine to form an amine
selected from the group consisting of an acyclic secondary amine, a
cyclic tertiary amine, a substituted acyclic amine, and a
substituted cyclic amine.
9. The porous material of claim 8, wherein said haloalkyl is
fluoromethyl, chloromethyl, bromomethyl or iodomethyl.
10. The porous material of claim 8, wherein the appropriate
starting amine is a primary amine selected from the group
consisting of methylamine, ethylamine, propylamine, isopropylamine,
butylamine, sec-butylamine, iso-butylamine, tert-butylamine,
pentylamine, 1,1-dimethylpropylamine, 1,2-dimethylpropylamine,
1-ethylpropylamine, 2-methylbutylamine, isopentylamine, hexylamine,
1,3-dimethylbutylamine, 3,3-dimethylamine, heptylamine,
2-aminoheptane, octylamine, 1,5-dimethylhexylamine,
2-ethylhexylamine, 1-methylheptylamine, tert-octylamine,
nonylamine, decylamine, undecylamine, dodecylamine, tridecylamine,
tetradecylamine, pentadecylamine, hexadecylamine, heptadecylamine,
octadecylamine, nonadecylamine, eicosylamine, azirane, azetane,
azolane, azinane, azepane, azocane, azonane, azecane, diazatene,
diazolane, diazinane, N-methyldiazinane, diazepane, diazocane,
diazonane, diazecane, oxazetane, oxazolane, oxazinane, oxazepane,
oxazocane, oxazonane, oxazecane, thiazetane, thiazolane,
thiazinane, thiazepane, thiazocane, thiazonane, thiazecane,
imidazole, benzylamine, N-methylbenzylamine, N-ethylbenzylamine,
N-propylbenzylamine, N-butylbenzylamine, N-pentylbenzylamine,
N-hexylbenzylamine, N-heptylbenzylamine, N-octylbenzylamine,
N-nonylbenzylamine, N-decylbenzylamine, N-undecylbenzylamine,
N-dodecylbenzylamine, N-tridecylbenzylamine,
N-tetradecylbenzylamine, N-pentadecylbenzylamine,
N-hexadecylbenzylamine, N-heptadecylbenzylamine,
N-octadecylbenzylamine, dibenzylamine, aniline, N-methylaniline,
N-ethylaniline, N-propylaniline, N-butylaniline, N-pentylaniline,
N-hexylaniline, N-heptylaniline, N-octylaniline, N-nonylaniline,
N-decylaniline, N-undecylaniline, N-dodecylaniline,
N-tridecylaniline, N-tetradecylaniline, N-pentadecylaniline,
N-hexadecylaniline, N-heptadecylaniline, N-octadecylaniline,
bis(2,2,2-trifluoromethyl)amine, phenethylamine,
N-methylphenethylamine, 4-methylphenethylamine,
3-phenylpropylamine, 1-methyl-3-phenylpropylamine,
N-isopropylbenzylamine, and 4-phenylbutylamine.
11.-27. (canceled)
28. The porous material of claim 1, wherein the porous material
comprises a monolith that comprises said copolymer.
29. (canceled)
30. A porous material comprising a copolymer having the formula I:
-(-A-).sub.n-(-B--).sub.n--(--C--).sub.p-- (I) and salts thereof,
wherein the order of repeat units A, B and C may be random, block,
or a combination of random and block; wherein 1 100 < ( p + n )
m < 100 1 ##EQU00003## and ##EQU00003.2## 1 500 < p n <
100 1 ##EQU00003.3## wherein A is selected from the group
consisting of ##STR00011## wherein B is selected from the group
consisting of ##STR00012## wherein C is modified A, wherein
modified A is selected from the group consisting of ##STR00013##
and wherein X is --CR.sub.1R.sub.2NR.sub.3R.sub.4 wherein: R.sub.1
and R.sub.2 are the same or different and each is hydrogen or
C.sub.1-C.sub.6 alkyl; R.sub.3 and R.sub.4 are the same or
different and each is hydrogen, an electron withdrawing group,
C.sub.1-C.sub.20 alkyl, C.sub.1-C.sub.20 alkyl substituted by an
electron withdrawing group, or R.sub.3 and R.sub.4 taken together
form a carbocyclic ring or a heterocyclic ring, wherein the
carbocyclic ring or heterocyclic ring can be substituted by an
electron withdrawing group, provided that (i) R.sub.1, R.sub.2,
R.sub.3 and R.sub.4 are not all hydrogen; (ii) if R.sub.1 and
R.sub.2 are hydrogen, then R.sub.3 and R.sub.4 are not both
unsubstituted C.sub.1-C.sub.20 alky; and (iii) if R.sub.1 and
R.sub.2 are hydrogen, and either of R.sub.3 and R.sub.4 is
hydrogen, then the other of R.sub.3 and R.sub.4 is not
polyethylenimine.
31.-49. (canceled)
50. The porous material of claim 30, wherein the copolymer is
##STR00014## ##STR00015##
51.-54. (canceled)
55. A porous material comprising a copolymer selected from the
group consisting of: ##STR00016## ##STR00017##
56. The porous material of claim 55, wherein the porous material
comprises a porous particle that comprises said copolymer.
57. The porous material of claim 55, wherein the porous material
comprises a monolith that comprises said copolymer.
58. A solid phase extraction or chromatography material comprising
the porous material of claim 1.
59.-71. (canceled)
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application
Ser. No. 12/790,795, filed May 28, 2010, allowed, which is a
continuation of U.S. patent application Ser. No. 10/782,397, filed
Feb. 18, 2004, now issued as U.S. Pat. No. 7,731,844, which is a
continuation-in-part of U.S. application Ser. No. 10/169,546, now
issued as U.S. Pat. No. 7,232,520, which entered the U.S. national
stage on Jan. 16, 2003, as the U.S. national phase application of
PCT international application No. PCT/US99/13241, filed on Jun. 10,
1999, which claims the benefit of U.S. provisional application Ser.
No. 60/089,153, filed on Jun. 12, 1998. The present application
also contains subject matter that is related to that disclosed and
claimed in U.S. application Ser. No. 09/505,456, filed on Feb. 11,
2000, now U.S. Pat. No. 6,322,695, which is a continuation-in-part
of PCT international application No. PCT/US99/13241, filed on Jun.
10, 1999, which claims priority to U.S. provisional application
Ser. No. 60/089,153, filed on Jun. 12, 1998. The disclosures of all
the aforementioned patent applications and the aforementioned
patent are incorporated herein in their entireties by this
reference.
BACKGROUND OF THE INVENTION
[0002] Solid phase extraction (SPE) is a chromatographic technique
that is widely used, e.g., for preconcentration and cleanup of
analytical samples, for purification of various chemicals, and for
removal of toxic or valuable substances from aqueous solutions. SPE
is usually performed using a column or cartridge containing an
appropriate material or sorbent. SPE procedures have been developed
using sorbents that can interact with analytes by hydrophobic,
ion-exchange, chelation, sorption, and other mechanisms, to bind
and remove the analytes from fluids.
[0003] Because different SPE applications can require different
sorbents, there is a need for sorbents with novel properties that
have unique selectivities. These include superior wetting
characteristics, selective capture of analytes of interest, and
non-retention of interfering analytes. Sorbents comprising porous
particles having the aforementioned properties are described in WO
99/64480 and in U.S. Pat. No. 6,322,695B1.
[0004] However, a problem associated with porous particles is the
passage or leaching of particles through the retaining frit of the
separation device into the sample of interest. In addition to
contamination of the sample, the passed particles can further
negatively impact test methods and the HPLC systems that are used
to test the samples. For example, the particles may clog or block
in line filters or column frits which in turn lead to high system
backpressures and ultimately HPLC pump shutdown.
[0005] Monolith materials have been developed in an attempt to
overcome the problem of particle passage or leaching through frits.
These include polymeric monoliths such as polymethacrylate
monoliths (U.S. Pat. No. 5,453,185, U.S. Pat. No. 5,728,457);
polystyrene -DVB monoliths (U.S. Pat. No. 4,889,632, U.S. Pat. No.
4,923,610, U.S. Pat. No. 4,952,349); charge incorporated
polymethacrylate monoliths for the application of reversed-phase
ion-pairing chromatography (U.S. Pat. No. 6,238,565); monoliths
based on ROMP metathesis (WO 00073782); and (EP 852334) continuous
monolith columns made from water-soluble polymerizable monomers,
such as vinyl, allyl, acrylic and methacrylic compounds, without
porogens but in the presence of high concentration of inorganic
salts such as ammonium sulfate.
[0006] Polymeric monoliths are chemically stable against strongly
alkaline and strongly acidic mobile phases, allowing flexibility in
the choice of mobile phase pH. However, the prior art monoliths do
not necessarily provide the unique selectivities and advantages
that are needed for a variety of chromatographic applications, in
particular SPE applications.
SUMMARY OF THE INVENTION
[0007] The invention is directed to novel porous materials that are
useful in chromatographic processes, e.g., solid phase extraction,
and that provide a number of advantages. Such advantages include
superior wetting characteristics, selective capture of analytes of
interest, and non-retention of interfering analytes. The invention
also provides novel porous materials that overcome the problems of
particle passage through frits.
[0008] Thus, in a first aspect, the invention provides a porous
material comprising a copolymer of at least one hydrophobic monomer
and at least one hydrophilic monomer, wherein said copolymer
further comprises at least one ion-exchange functional moiety
selected from the group consisting of an acyclic secondary amine
exclusive of polyethylenimine, a cyclic tertiary amine, a
substituted acyclic amine, and a substituted cyclic amine.
[0009] In a second aspect, the invention provides a copolymer
having the formula I:
-(-A-).sub.n-(-B--).sub.m--(--C--).sub.p-- (I)
and salts thereof,
[0010] wherein the order of repeat units A, B and C may be random,
block, or a combination of random and block;
[0011] wherein
1 100 < ( p + n ) m < 100 1 ##EQU00001## and ##EQU00001.2## 1
500 < p n < 100 1 ##EQU00001.3##
[0012] wherein A is selected from the group consisting of
##STR00001##
[0013] wherein B is selected from the group consisting of
##STR00002##
[0014] wherein C is modified A, wherein modified A is selected from
the group consisting of
##STR00003##
[0015] and
[0016] wherein X is --CR.sub.1R.sub.2NR.sub.3R.sub.4 wherein:
[0017] R.sub.1 and R.sub.2 are the same or different and each is
hydrogen or C.sub.1-C.sub.6 alkyl;
[0018] R.sub.3 and R.sub.4 are the same or different and each is
hydrogen, an electron withdrawing group, C.sub.1-C.sub.20 alkyl,
C.sub.1-C.sub.20 alkyl substituted by an electron withdrawing
group, or R.sub.3 and R.sub.4 taken together form a carbocyclic
ring or a heterocyclic ring, wherein the carbocyclic ring or
heterocyclic ring can be substituted by an electron withdrawing
group, provided that (i) R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are
not all hydrogen; (ii) if R.sub.1 and R.sub.2 are hydrogen, then
R.sub.3 and R.sub.4 are not both unsubstituted C.sub.1-C.sub.20
alky; and (iii) if R.sub.1 and R.sub.2 are hydrogen, and either of
R.sub.3 and R.sub.4 is hydrogen, then the other of R.sub.3 and
R.sub.4 is not polyethylenimine.
[0019] In accordance with the invention, the porous materials
disclosed herein can take the form of porous particles or
monoliths. Thus, in yet another aspect, the invention provides a
porous material comprising a porous particle that comprises a
copolymer described above with regard to the first and second
aspects of the invention. Likewise, the invention provides a porous
material comprising a porous monolith that comprises a copolymer
described above with regard to the first and second aspects of the
invention.
[0020] In another aspect, the invention also provides solid phase
extraction and chromatography materials comprising porous materials
of the invention.
[0021] In yet another aspect, the invention provides a separation
device comprising a porous material of the invention. In a related
aspect, the invention provides a solid phase extraction cartridge
comprising a porous materials according to the invention.
[0022] The invention also provides a method for removing or
isolating a component from a mixture. The method comprises
contacting the mixture with a chromatographic material comprising
the porous material according to the invention, to thereby remove
or isolate the component from the mixture.
[0023] In another aspect, the invention provides a method for
determining the level of a component in a mixture. The method
comprises contacting the mixture with a chromatographic material
comprising a porous material according to the invention under
conditions that allow for sorption of the component onto the porous
material; washing the chromatographic material having the sorbed
component with a solvent under conditions so as to desorb the
component from the porous materials; and determining the level of
the desorbed component.
BRIEF DESCRIPTION OF THE DRAWINGS
[0024] FIG. 1 depicts the total ion chromatogram (TIC) obtained
using the product of Example 3b.
[0025] FIG. 2 depicts the total ion chromatogram (TIC) obtained
using the product of Example 3f.
DETAILED DESCRIPTION OF THE INVENTION
[0026] Definitions
[0027] The present invention will be more fully illustrated by
reference to the definitions set forth below.
[0028] The term "alicyclic group" includes closed ring structures
of three or more carbon atoms. Alicyclic groups include
cycloparaffins or naphthenes that are saturated cyclic
hydrocarbons, cycloolefins that are unsaturated with two or more
double bonds, and cycloacetylenes, which have a triple bond. They
do not include aromatic groups. Examples of cycloparaffins include
cyclopropane, cyclohexane, and cyclopentane. Examples of
cycloolefins include cyclopentadiene and cyclooctatetraene.
Alicyclic groups also include fused ring structures and substituted
alicyclic groups such as alkyl substituted alicyclic groups. In the
instance of the alicyclics such substituents may further comprise a
lower alkyl, a lower alkenyl, a lower alkoxy, a lower alkylthio, a
lower alkylamino, a lower alkylcarboxyl, a nitro, a hydroxyl,
--CF.sub.3, --CN, or the like.
[0029] The term "aliphatic group" includes organic compounds
characterized by straight or branched chains, typically having
between 1 and 22 carbon atoms. Aliphatic groups include alkyl
groups, alkenyl groups and alkynyl groups. In complex structures,
the chains may be branched or cross-linked. Alkyl groups include
saturated hydrocarbons having one or more carbon atoms, including
straight-chain alkyl groups and branched-chain alkyl groups. Such
hydrocarbon moieties may be substituted on one or more carbons
with, for example, a halogen, a hydroxyl, a thiol, an amino, an
alkoxy, an alkylcarboxy, an alkylthio, or a nitro group. Unless the
number of carbons is otherwise specified, "lower aliphatic" as used
herein means an aliphatic group, as defined above (e.g., lower
alkyl, lower alkenyl, lower alkynyl), but having from one to six
carbon atoms. Representative of such lower aliphatic groups, e.g.,
lower alkyl groups, are methyl, ethyl, n-propyl, isopropyl,
2-chloropropyl, n-butyl, sec-butyl, 2-aminobutyl, isobutyl,
tert-butyl, 3-thiopentyl, and the like. As used herein, the term
"nitro" means --NO.sub.2; the term "halogen" designates --F, --Cl,
--Br or --I; the term "thiol" means SH; and the term "hydroxyl"
means --OH.
[0030] The terms "alkenyl" and "alkynyl" refer to unsaturated
aliphatic groups analogous to alkyls, but which contain at least
one double or triple bond respectively. Suitable alkenyl and
alkynyl groups include groups having 2 to about 12 carbon atoms,
preferably from 1 to about 6 carbon atoms.
[0031] The term "alkoxy" as used herein means an alkyl group, as
defined above, having an oxygen atom attached thereto.
Representative alkoxy groups include groups having 1 to about 12
carbon atoms, preferably 1 to about 6 carbon atoms, e.g., methoxy,
ethoxy, propoxy, tert-butoxy and the like.
[0032] The term "alkyl" includes saturated aliphatic groups,
including straight-chain alkyl groups, branched-chain alkyl groups,
cycloalkyl (alicyclic) groups, alkyl substituted cycloalkyl groups,
and cycloalkyl substituted alkyl groups. In certain embodiments, a
straight chain or branched chain alkyl has 30 or fewer carbon atoms
in its backbone, e.g., C.sub.1-C.sub.30 for straight chain or
C.sub.3-C.sub.30 for branched chain. In certain embodiments, a
straight chain or branched chain alkyl has 20 or fewer carbon atoms
in its backbone, e.g., C.sub.1-C.sub.20 for straight chain or
C.sub.3-C.sub.20 for branched chain, and more preferably 18 or
fewer. Likewise, preferred cycloalkyls have from 4-10 carbon atoms
in their ring structure, and more preferably have 4-7 carbon atoms
in the ring structure. The term "lower alkyl" refers to alkyl
groups having from 1 to 6 carbons in the chain, and to cycloalkyls
having from 3 to 6 carbons in the ring structure.
[0033] Moreover, the term "alkyl" (including "lower alkyl") as used
throughout the specification and claims includes both
"unsubstituted alkyls" and "substituted alkyls," the latter of
which refers to alkyl moieties having substituents replacing a
hydrogen on one or more carbons of the hydrocarbon backbone. Such
substituents may include, for example, halogen, hydroxyl,
alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy,
aryloxycarbonyloxy, alkylcarbonyl, alkoxycarbonyl, aminocarbonyl,
alkylthiocarbonyl, alkoxyl, cyano, alkyl amino, arylamino,
diarylamino, and alkylarylamino, acylamino (including
alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido),
amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate,
sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido,
heterocycyl, aralkyl, or an aromatic or heteroaromatic moiety. It
will be understood by those skilled in the art that the moieties
substituted on the hydrocarbon chain may themselves be substituted,
if appropriate.
[0034] Cycloalkyls may be further substituted, e.g., with the
substituents described above. An "aralkyl" moiety is an alkyl
substituted with an aryl, e.g., having 1 to 3 separate or fused
rings and from 6 to about 18 carbon ring atoms, e.g., phenylmethyl
(benzyl).
[0035] The term "alkylamino" as used herein means an alkyl group,
as defined above, having an amino group attached thereto. Suitable
alkylamino groups include groups having 1 to about 12 carbon atoms,
preferably from 1 to about 6 carbon atoms. The term "amino," as
used herein, refers to an unsubstituted or substituted moiety of
the formula --NR.sub.aR.sub.b, in which R.sub.a and R.sub.b are
each independently hydrogen, alkyl, aryl, or heterocyclyl, but
R.sub.a and R.sub.b are both not alkyl, or R.sub.a and R.sub.b,
taken together with the nitrogen atom to which they are attached,
form a cyclic moiety having from 3 to 8 atoms in the ring. An
"amino-substituted amino group" refers to an amino group in which
at least one of R.sub.a and R.sub.b, is further substituted with an
amino group.
[0036] Thus, the terms "alkylamino" and "amino" include acyclic
primary amines such methylamine, ethylamine, propylamine,
isopropylamine, butylamine, sec-butylamine, iso-butylamine,
tert-butylamine, pentylamine, 1,1-dimethylpropylamine,
1,2-dimethylpropylamine, 1-ethylpropylamine, 2-methylbutylamine,
isopentylamine, hexylamine, 1,3-dimethylbutylamine,
3,3-dimethylamine, heptylamine, 2-aminoheptane, octylamine,
1,5-dimethylhexylamine, 2-ethylhexylamine, 1-methylheptylamine,
tert-octylamine, nonylamine, decylamine, undecylamine,
dodecylamine, tridecylamine, tetradecylamine, pentadecylamine,
hexadecylamine, heptadecylamine, octadecylamine, nonadecylamine,
and eicosylamine. Preferred primary amines include propylamine,
isopropylamine, butylamine, sec-butylamine, iso-butylamine,
pentylamine, isopentylamine, hexylamine, heptylamine,
2-aminoheptane, octylamine, 2-ethylhexylamine, dodecylamine, or
octadecylamine.
[0037] The terms "alkylamino" and "amino" also include cyclic
secondary amines such as azirane, azetane, azolane, azinane,
azepane, azocane, azonane, azecane, diazatene, diazolane,
diazinane, N-methyldiazinane, diazepane, diazocane, diazonane,
diazecane, and imidazole. Preferred cyclic secondary amines include
azinane, diazinane and N-methyl-diazinane.
[0038] The term "alkylthio" refers to an alkyl group, as defined
above, having a sulfhydryl group attached thereto. Suitable
alkylthio groups include groups having 1 to about 12 carbon atoms,
preferably from 1 to about 6 carbon atoms. The term "alkylcarboxyl"
as used herein means an alkyl group, as defined above, having a
carboxyl group attached thereto.
[0039] The term "aromatic group" includes unsaturated cyclic
hydrocarbons containing one or more rings. Aromatic groups include
5- and 6-membered single-ring groups which may include from zero to
four heteroatoms, for example, benzene, pyrrole, furan, thiophene,
imidazole, oxazole, thiazole, triazole, pyrazole, pyridine,
pyrazine, pyridazine and pyrimidine, and the like. The aromatic
ring may be substituted at one or more ring positions with, for
example, a halogen, a lower alkyl, a lower alkenyl, a lower alkoxy,
a lower alkylthio, a lower alkylamino, a lower alkylcarboxyl, a
nitro, a hydroxyl, --CF.sub.3, --CN, or the like.
[0040] The term "aryl" includes 5- and 6-membered single-ring
aromatic groups that may include from zero to four heteroatoms, for
example, unsubstituted or substituted benzene, pyrrole, furan,
thiophene, imidazole, oxazole, thiazole, triazole, pyrazole,
pyridine, pyrazine, pyridazine and pyrimidine, and the like. Aryl
groups also include polycyclic fused aromatic groups such as
naphthyl, quinolyl, indolyl, and the like. The aromatic ring may be
substituted at one or more ring positions with such substituents,
e.g., as described above for alkyl groups. Suitable aryl groups
include unsubstituted and substituted phenyl groups. The term
"aryloxy" as used herein means an aryl group, as defined above,
having an oxygen atom attached thereto. The term "aralkoxy" as used
herein means an aralkyl group, as defined above, having an oxygen
atom attached thereto. Suitable aralkoxy groups have 1 to 3
separate or fused rings and from 6 to about 18 carbon ring atoms,
e.g., O-benzyl.
[0041] The term "block ordering" is intended to include ordering in
which individual units are joined in a pattern or repeated
sequence.
[0042] The term "conjugate acid of an amine" describes a protonated
amine that is positively charged.
[0043] The term "copolymer" is intended to include a polymer
comprising two or more different monomers.
[0044] The term "electron withdrawing group" describes a
substituent or group that has the effect of lowering the average
pK.sub.a of the conjugate acid of an amine substituted with the
electron withdrawing group as compared to the conjugate acid of
that amine without the electron withdrawing group. Electron
withdrawing groups in accordance with the invention include
halogens, aromatic groups, unsaturated groups, ethers, thioethers,
nitriles, nitro groups, esters, amides, carbamates, ureas,
carbonates, sulfonamides, sulfones, and sulfoxides. In addition,
the term is intended to include heteroatoms that substitute for
ring carbon atoms in a heterocycle. Preferred electron withdrawing
groups include halogens, ethers, or an aromatic group.
[0045] The term "haloalkyl" is intended to include alkyl groups as
defined above that are mono-, di- or polysubstituted by halogen,
e.g., chloromethyl, fluoromethyl, bromomethyl, iodomethyl, and
trifluoromethyl.
[0046] The term "heteroatom" as used herein means an atom of any
element other than carbon or hydrogen. Preferred heteroatoms are
nitrogen, oxygen, sulfur and phosphorus.
[0047] The term "heterocyclic group" includes closed ring
structures in which one or more of the atoms in the ring is an
element other than carbon, for example, nitrogen, sulfur, or
oxygen. Heterocyclic groups may be saturated or unsaturated and
heterocyclic groups such as pyrrole and furan may have aromatic
character. They include fused ring structures such as quinoline and
isoquinoline. Other examples of heterocyclic groups include
pyridine and purine. Heterocyclic groups may also be substituted at
one or more constituent atoms with, for example, a halogen, a lower
alkyl, a lower alkenyl, a lower alkoxy, a lower alkylthio, a lower
alkylamino, a lower alkylcarboxyl, a nitro, a hydroxyl, --CF.sub.3,
--CN, or the like. Suitable heteroaromatic and heteroalicyclic
groups generally will have 1 to 3 separate or fused rings with 3 to
about 8 members per ring and one or more N, O or S atoms, e.g.,
coumarinyl, quinolinyl, pyridyl, pyrazinyl, pyrimidyl, furyl,
pyrrolyl, thienyl, thiazolyl, oxazolyl, imidazolyl, indolyl,
benzofuranyl, benzothiazolyl, tetrahydrofuranyl, tetrahydropyranyl,
piperidinyl, morpholino and pyrrolidinyl.
[0048] Thus, the term "heterocyclic group" includes moieties such
as azirane, azetane, azolane, azinane, azepane, azocane, azonane,
and azecane that are heterocyclic molecules containing a single
nitrogen within a 3-, 4-, 5-, 6-, 7-, 8-, 9-, and 10-membered ring
respectively. These molecules may also have additional fused rings
of the same or a different structure and may also have
unsaturation. These molecules may be further substituted with a
variety groups including aliphatic groups, alicyclic groups,
heterocyclic groups, aromatic groups, and the like.
[0049] The term "heterocyclic group" also includes moieties such as
diazatene, diazolane, diazinane, diazepane, diazocane, diazonane,
and diazecane that are heterocyclic molecules containing two
nitrogens within a 4-, 5-, 6-, 7-, 8-, 9- and 10-membered ring
respectively. These molecules may also have additional fused rings
of the same or a different structure and may also have
unsaturation. These molecules may be further substituted with a
variety groups including aliphatic groups, alicyclic groups,
heterocyclic groups, aromatic groups, and the like.
N-methylpiperazine is an example of a substituted diazinane.
Imidazole is an example of an unsaturated diazolane.
[0050] The term "heterocyclic group" further includes moieties such
as oxazetane, oxazolane, oxazinane, oxazepane, oxazocane,
oxazonane, and oxazecane" that are heterocyclic molecules
containing one oxygen and one nitrogen within a 4-, 5-, 6-, 7-, 8-,
9-, and 10-membered ring respectively. These molecules may also
have additional fused rings of the same or a different structure
and may also have unsaturation. These molecules may be further
substituted with a variety groups including aliphatic groups,
alicyclic groups, heterocyclic groups, aromatic groups, and the
like. Morpholine is an example of an oxazinane.
[0051] The term "heterocyclic group" also includes moieties such as
thiazetane, thiazolane, thiazinane, thiazepane, thiazocane,
thiazonane, and thiazecane that are heterocyclic molecules
containing one sulfur and one nitrogen within a 4-, 5- 6-, 7-, 8-,
9, and 10-membered ring respectively. These molecules may also have
additional fused rings of the same or a different structure and may
also have unsaturation. These molecules may be further substituted
with a variety groups including aliphatic groups, alicyclic groups,
heterocyclic groups, aromatic groups, and the like.
[0052] The term "hydrophilic" describes having an affinity for,
attracting, adsorbing or absorbing water.
[0053] The term "hydrophobic" describes lacking an affinity for,
repelling, or failing to adsorb or absorb water.
[0054] The term "ion-exchange functional group" is intended to
include a group where the counter-ion is partially free and can
readily be exchanged for other ions of the same sign.
[0055] The term "mole percent" describes the mole fraction,
expressed as a percent, of the monomer of interest relative to the
total moles of the various (two or more) monomers which compose the
copolymer of the porous material of the invention.
[0056] The term "monolith" is intended to include a porous,
three-dimensional material having a continuous interconnected pore
structure in a single piece. A monolith is prepared, for example,
by casting precursors into a mold of a desired shape. The term
monolith is meant to be distinguished from a collection of
individual particles packed into a bed formation, in which the end
product still comprises individual particles in bed formation.
[0057] The term "monomer" is intended to include a molecule
comprising one or more polymerizable functional groups prior to
polymerization, or a repeating unit of a polymer.
[0058] The term "porous material" is intended to include a member
of a class of porous crosslinked polymers penetrated by pores
through which solutions can diffuse. Pores are regions between
densely packed polymer chains.
[0059] The term "random ordering" is intended to include ordering
in which individual units are joined randomly.
[0060] The term "solid phase extraction" is intended to include a
process employing a solid phase for isolating classes of molecular
species from fluid phases such as gases and liquids by, e.g.,
sorption, ion-exchange, chelation, size exclusion (molecular
filtration), affinity or ion pairing mechanisms.
[0061] The term "sorption" describes the ability of a material to
take up and hold another material by absorption or adsorption.
[0062] Compositions and Methods of the Invention
[0063] The invention provides a porous material comprising a
copolymer of a least one hydrophobic monomer and at least one
hydrophilic monomer, wherein said copolymer further comprises at
least one ion-exchange functional moiety selected from the group
consisting of an acyclic secondary amine exclusive of
polyethylenimine, a cyclic tertiary amine, a substituted acyclic
amine, and a substituted cyclic amine. Preferably, the porous
material has a specific surface area in the range from about 50 to
about 850 square meters per gram and pores having a diameter
ranging from about 0.5 nm to about 100 nm. In certain embodiments,
the porous material is incorporated in a matrix.
[0064] In certain embodiments, the porous materials of the
invention take the form of porous particles, e.g., beads, pellets,
or any other form desirable for use. The porous particles can have,
e.g., a spherical shape, a regular shape or an irregular shape.
Preferably, the particles are beads having a diameter in the range
from about 3 to about 500 .mu.m, preferably from about 20 to about
200 .mu.m.
[0065] In other embodiments, the porous materials of the invention
take the form of porous monoliths. In certain embodiments, the
monoliths have the following characteristics: surface area ranging
from about 50 to about 800 m.sup.2/g, more particularly about 300
to about 700 m.sup.2/g; pore volume ranging from about 0.2 to about
2.5 cm.sup.3/g, more particularly about 0.4 to about 2.0
cm.sup.3/g, still more particularly about 0.6 to about 1.4
cm.sup.3/g; and pore diameter ranging from about 20 to about 500
.ANG., more particularly about 50 to 300 .ANG., still more
particularly about 80 to about 150 .ANG..
[0066] The porous materials of the invention comprise a copolymer
comprising a least one hydrophobic monomer and at least one
hydrophilic monomer. In certain embodiments, the copolymer of the
invention is non-sulfonated.
[0067] In certain embodiments the hydrophobic monomer comprises an
aromatic carbocyclic group, e.g., a phenyl group or a phenylene
group, or a straight chain C.sub.2-C.sub.18-alkyl group or a
branched chain C.sub.2-C.sub.18-alkyl group. The hydrophobic
monomer can be, e.g., styrene or divinylbenzene. A preferred
copolymer is a poly(divinylbenzene-co-N-vinylpyrrolidone).
[0068] In certain embodiments, the hydrophilic monomer comprises a
heterocyclic group, e.g., a saturated, unsaturated or aromatic
heterocyclic group. Examples include nitrogen-containing
heterocyclic groups, e.g., a pyridyl group, e.g., 2-vinylpyridine,
3-vinylpyridine or 4-vinylpyridine, or a pyrrolidonyl group, e.g.,
N-vinylpyrrolidone.
[0069] In one embodiment, the hydrophobic monomer is divinylbenzene
or styrene, and the hydrophilic monomer is N-vinylpyrrolidone or
N-vinyl acetamide. In a specific embodiment, the copolymer is a
poly(divinylbenzene-co-N-vinylpyrrolidone). Preferably, the porous
material comprises at least about 12 mole percent
N-vinylpyrrolidone. More preferably, the porous material comprises
at least about 30 mole percent N-vinylpyrrolidone.
[0070] The invention also provides porous materials wherein the
hydrophobic monomer is substituted by at least one haloalkyl group,
and the ion-exchange functional moiety is formed by reaction of the
haloalkyl group with an appropriate starting amine to form an amine
selected from the group consisting of an acyclic secondary amine, a
cyclic tertiary amine, a substituted acyclic amine, and a
substituted cyclic amine. In certain embodiments, the haolalkyl
group is fluoromethyl, choromethyl, bromomethyl or iodomethyl. In
one embodiment, the haloalkyl group is chloromethyl.
[0071] Examples of primary amines that can be used in accordance
with the invention include methylamine, ethylamine, propylamine,
isopropylamine, butylamine, sec-butylamine, iso-butylamine,
tert-butylamine, pentylamine, 1,1-dimethylpropylamine,
1,2-dimethylpropylamine, 1-ethylpropylamine, 2-methylbutylamine,
isopentylamine, hexylamine, 1,3-dimethylbutylamine,
3,3-dimethylamine, heptylamine, 2-aminoheptane, octylamine,
1,5-dimethylhexylamine, 2-ethylhexylamine, 1-methylheptylamine,
tert-octylamine, nonylamine, decylamine, undecylamine,
dodecylamine, tridecylamine, tetradecylamine, pentadecylamine,
hexadecylamine, heptadecylamine, octadecylamine, nonadecylamine,
and eicosylamine. In certain embodiments, the primary amine is
propylamine, isopropylamine, butylamine, sec-butylamine,
iso-butylamine, pentylamine, isopentylamine, hexylamine,
heptylamine, 2-aminoheptane, octylamine, 2-ethylhexylamine,
dodecylamine, or octadecylamine.
[0072] Examples of cyclic secondary amines in accordance with the
invention include azirane, azetane, azolane, azinane, azepane,
azocane, azonane, azecane, diazatene, diazolane, diazinane,
diazepane, diazocane, diazonane, diazecane, imidazole, oxazetane,
oxazolane, oxazinane, oxazepane, oxazocane, oxazonane, oxazecane,
thiazetane, thiazolane, thiazinane, thiazepane, thiazocane,
thiazonane, and thiazecane. In one embodiment, the cyclic secondary
amine is 1,4-oxazinane. In another embodiment, the cyclic secondary
amine is azinane. In yet another embodiment, the cyclic secondary
amine is diazinane.
[0073] In accordance with the invention, the ion-exchange
functional moiety can be formed from a substituted acyclic amine or
a substituted cyclic amine. The substitution can be at any of the
ring atoms, including heteroatoms. For example, in certain
embodiments, the ion-exchange functional moiety is a substituted
cyclic secondary amine, e.g., N-methyldiazinane and
4-methylpiperidine.
[0074] In other embodiments, the aforesaid amines are
advantageously substituted by an electron withdrawing group. In
certain embodiments, the electron withdrawing group is selected
from the group consisting of halogens, aromatic groups, unsaturated
groups, ethers, thioethers, nitriles, nitro groups, esters, amides,
carbamates, ureas, carbonates, sulfonamides, sulfones, sulfoxides
and heteroatoms, e.g., N, O and S. In certain embodiments, the
electron withdrawing group is a halogen, an ether, or an aromatic
group.
[0075] In accordance with the invention, the electron withdrawing
group of the amine has the effect of lowering the average pK.sub.a
of the conjugate acid of the amine as compared to the conjugate
acid of the amine without the electron withdrawing group. In
certain embodiments, the pK.sub.a ranges from about 5 to about
7.
[0076] In certain embodiments, the acyclic amine substituted with
an electron withdrawing group includes benzylamine,
N-methylbenzylamine, N-ethylbenzylamine, N-propylbenzylamine,
N-butylbenzylamine, N-pentylbenzylamine, N-hexylbenzylamine,
N-heptylbenzylamine, N-octylbenzylamine, N-nonylbenzylamine,
N-decylbenzylamine, N-undecylbenzylamine, N-dodecylbenzylamine,
N-tridecylbenzylamine, N-tetradecylbenzylamine,
N-pentadecylbenzylamine, N-hexadecylbenzylamine,
N-heptadecylbenzylamine, N-octadecylbenzylamine, dibenzylamine,
aniline, N-methylaniline, N-ethylaniline, N-propylaniline,
N-butylaniline, N-pentylaniline, N-hexylaniline, N-heptylaniline,
N-octylaniline, N-nonylaniline, N-decylaniline, N-undecylaniline,
N-dodecylaniline, N-tridecylaniline, N-tetradecylaniline,
N-pentadecylaniline, N-hexadecylaniline, N-heptadecylaniline,
N-octadecylaniline, bis(2,2,2-trifluoromethyl)amine,
phenethylamine, N-methylphenethylamine, 4-methylphenethylamine,
3-phenylpropylamine, 1-methyl-3-phenylpropylamine,
N-isopropylbenzylamine, and 4-phenylbutylamine. In certain
preferred embodiments, the acyclic amine substituted with an
electron withdrawing group is benzylamine, N-methylbenzylamine, or
phenethylamine. In a preferred embodiment, the acyclic amine
substituted with an electron withdrawing group is
N-methylbenzylamine.
[0077] In other embodiments, cyclic secondary amines substituted
with an electron withdrawing group include oxazetane, oxazolane,
oxazinane, oxazepane, oxazocane, oxazonane, oxazecane, thiazetane,
thiazolane, thiazinane, thiazepane, thiazocane, thiazonane, and
thiazecane. In one embodiment, the cyclic secondary amine is
1,4-oxazinane. In these embodiments, one of ordinary skill in the
art will appreciate that the electron withdrawing group is a second
heteroatom that has substituted for a carbon atom in the ring. For
example, the ring carbon adjacent to the nitrogen atom in azetidine
is substituted by an oxygen to yield oxazetane, an amine
encompassed by the term "cyclic secondary amine substituted with an
electron withdrawing group".
[0078] The porous materials, in either porous particle or monolith
form, are advantageously used for solid phase extraction or
chromatography. In a one embodiment, the porous material comprises
at least one porous particle, and more preferably a plurality of
porous particles. In one embodiment, the porous material comprises
the copolymer poly(divinylbenzene-co-N-vinylpyrrolidone). In a
related embodiment, the poly(divinylbenzene-co-N-vinylpyrrolidone)
has ion-exchange functional moieties present at a concentration of
about 0.01 to about 1.0 milliequivalents per gram of porous
material.
[0079] In another aspect, porous materials of the invention, in
either porous particle or monolith form, comprise novel copolymers.
These copolymers have the formula I:
-(-A-).sub.n-(-B--).sub.m--(--C--).sub.p-- (I)
[0080] and salts thereof,
[0081] wherein the order of repeat units A, B and C may be random,
block, or a combination of random and block;
[0082] wherein
1 100 < ( p + n ) m < 100 1 ##EQU00002## and ##EQU00002.2## 1
500 < p n < 100 1 ##EQU00002.3##
[0083] wherein A is selected from the group consisting of
##STR00004##
[0084] wherein B is selected from the group consisting of
##STR00005##
[0085] wherein C is modified A, wherein modified A is selected from
the group consisting of
##STR00006##
[0086] and
[0087] wherein X is --CR.sub.1R.sub.2NR.sub.3R.sub.4 wherein:
[0088] R.sub.1 and R.sub.2 are the same or different and each is
hydrogen or C.sub.1-C.sub.6 alkyl;
[0089] R.sub.3 and R.sub.4 are the same or different and each is
hydrogen, an electron withdrawing group, C.sub.1-C.sub.20 alkyl,
C.sub.1-C.sub.20 alkyl substituted by an electron withdrawing
group, or R.sub.3 and R.sub.4 taken together form a carbocyclic
ring or a heterocyclic ring, wherein the carbocyclic ring or
heterocyclic ring can be substituted by an electron withdrawing
group, provided that (i) R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are
not all hydrogen; (ii) if R.sub.1 and R.sub.2 are hydrogen, then
R.sub.3 and R.sub.4 are not both unsubstituted C.sub.1-C.sub.20
alky; and (iii) if R.sub.1 and R.sub.2 are hydrogen, and either of
R.sub.3 and R.sub.4 is hydrogen, then the other of R.sub.3 and
R.sub.4 is not polyethylenimine.
[0090] In certain embodiments, the repeat unit of A is
divinylbenzene or styrene. In certain embodiments, the repeat unit
of B is N-vinylpyrrolidone or N-vinyl acetamide. In a preferred
embodiment, the copolymer is a
poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer.
[0091] In one embodiment, A and B form a porous particle. In
another embodiment, A and B form a porous monolith.
[0092] The ion-exchange functional group is represented by X in
formula I above. Preferably, the ion-exchange functional groups are
present at a concentration of about 0.01 to about 1.0, more
preferably at a concentration of about 0.2 to about 0.8, more
preferably yet at a concentration of about 0.4 to about 0.6, and
still more preferably at a concentration of about 0.5
milliequivalents per gram of porous material.
[0093] In certain embodiments, the ion-exchange functional moiety
represented by X is an amine selected from the group consisting of
an acyclic secondary amine exclusive of polyethylenimine, a cyclic
tertiary amine, a substituted acyclic amine, and a substituted
cyclic amine. In such embodiments, the ion-exchange functional
moiety is formed by reaction of a haloalkyl group with an
appropriate starting amine to form the amine selected from the
group consisting of an acyclic secondary amine, a cyclic tertiary
amine, a substituted acyclic amine, and a substituted cyclic
amine.
[0094] More particularly, the haloalkyl group, either introduced at
the appropriate position on the phenyl ring after copolymerization
(see Example 2 below) or as a pre-existing substituent on the
phenyl ring of repeat unit C (see Example 4 below), is reacted with
the appropriate starting amines (see Examples 3 and 5 below) in
accordance with reactions and under conditions well-known to those
of ordinary skill in the art. The appropriate starting amine can be
any of the primary amines, cyclic secondary amines, substituted
acyclic amines and substituted cyclic secondary amines described
above.
[0095] In certain embodiments of the copolymer of formula I above,
the electron withdrawing group is selected from the group
consisting of halogens, aromatic groups, e.g., pyridyl, hydroxy
groups, unsaturated groups, ethers, thioethers, nitriles, nitro
groups, esters, amides, carbamates, ureas, carbonates,
sulfonamides, sulfones, and sulfoxides. In certain embodiments, the
electron withdrawing group is a halogen, an ether, or an aromatic
group.
[0096] In accordance with the invention, X can be any of the
following moieties:
##STR00007##
[0097] Preferred copolymers of Formula I include the following:
##STR00008## ##STR00009##
[0098] The porous materials of the invention can be prepared, e.g.,
by functionalizing, i.e., chemically altering, a copolymer having
at least one hydrophobic repeat unit and at least one hydrophilic
repeat unit. The order of repeat units may be random, block, or
combinations of random and block.
[0099] The hydrophobic repeat unit may be derived from a variety of
hydrophobic monomer reagents possessing one or more polymerizable
moieties, capable of undergoing polymerization, e.g., a free
radical-mediated polymerization. Examples of hydrophobic monomers
include but are not limited to divinylbenzene, styrene,
ethylvinylbenzene, and vinylbenzylchloride. Preferably, the
hydrophobic monomer is divinylbenzene.
[0100] The hydrophilic repeat unit may be derived from a variety of
hydrophilic monomer reagents possessing one or more polymerizable
moieties, capable of undergoing polymerization, e.g., a free
radical-mediated polymerization. Examples of hydrophilic monomers
include but are not limited to N-vinylpyrrolidone,
N-vinylacetamide, N-vinylpyridine, methacrylate, methyl
methacrylate, vinyl acetate, acrylamide or methacrylamide.
Preferably, the hydrophilic monomer is N-vinylpyrrolidone.
[0101] The copolymer can be prepared via a number of processes and
mechanisms including, but not limited to, chain addition and step
condensation processes, radical, anionic, cationic, ring-opening,
group transfer, metathesis, and photochemical mechanisms. The
copolymer can be prepared via standard synthetic methods known to
those skilled in the art, e.g., as described in Example 1. Such a
copolymer, e.g., poly(divinylbenzene-co-N-vinylpyrrolidone), can be
functionalized by the addition of an ion-exchange functional group,
e.g., the X group defined above in formula I. In a preferred
embodiment, X is
##STR00010##
[0102] The novel materials of the invention, e.g., in the form of
porous particles or monoliths, can be used for solid phase
extraction and chromatography. Thus, the invention also provides a
porous material for solid phase extraction or chromatography
comprising at least one ion-exchange functional group, at least one
hydrophilic component and at least one hydrophobic component. The
ion-exchange functional groups enable the porous material to
interact with basic and cationic solutes. The hydrophilic polar
components enable the porous material to have polar interactions
and hydrogen bonding capabilities with solutes. The hydrophobic
components enable the porous material to have affinity towards
nonpolar solutes through hydrophobic interaction. Since the porous
materials of this invention have a combination of various
interaction forces towards solutes, they are very useful materials
for, e.g., solid phase extraction, ion-exchange, and liquid
chromatography applications. For example, these novel porous
materials can be used to bind, recover and/or remove solutes from
fluids.
[0103] The invention also provides a method for removing or
isolating a component, e.g., a solute, from a mixture. A solution
having a solute is contacted with a porous material of the
invention under conditions that allow for sorption of the solute to
the porous material.
[0104] The solute can be, e.g., any molecule having a hydrophobic,
hydrophilic, or ionic interaction or a combination of two or three
of these interactions. Preferably, the solute is an organic
compound of polarity suitable for adsorption onto the porous
material. Such solutes include, e.g., drugs, pesticides,
herbicides, toxins and environmental pollutants, e.g., resulting
from the combustion of fossil fuels or other industrial activity,
such as metal-organic compounds comprising a heavy metal such
mercury, lead or cadmium. The solutes can also be metabolites or
degradation products of the foregoing materials. Solutes also
include, e.g., biomolecules, such as proteins, peptides, hormones,
polynucleotides, vitamins, cofactors, metabolites, lipids and
carbohydrates.
[0105] The solution e.g., can comprise water, an aqueous solution,
or a mixture of water or an aqueous solution and a water-miscible
polar organic solvent, e.g., methanol, ethanol,
N,N-dimethylformamide, dimethylsulfoxide or acetonitrile. In a
preferred embodiment, the solution is an acidic, basic or neutral
aqueous, i.e., between about 1% and about 99% water by volume,
solution. The solution comprising the solute can, optionally,
further contain one or more additional solutes. In one embodiment,
the solution is an aqueous solution which includes a complex
variety of solutes. Solutions of this type include, e.g., blood,
plasma, urine, cerebrospinal fluid, synovial fluid and other
biological fluids, including, e.g., extracts of tissues, such as
liver tissue, muscle tissue, brain tissue or heart tissue. Such
extracts can be, e.g., aqueous extracts or organic extracts which
have been dried and subsequently reconstituted in water or in a
water/organic mixture. Solutions also include, e.g., ground water,
surface water, drinking water or an aqueous or organic extract of
an environmental sample, such as a soil sample. Other examples of
solutions include a food substance, such as a fruit or vegetable
juice or milk or an aqueous or aqueous/organic extract of a food
substance, such as fruit, vegetable, cereal or meat. Other
solutions include, e.g., natural products extractions from plants
and broths.
[0106] The solution can be contacted with the porous material in
any fashion which allows sorption of the solute to the porous
material, such as a batch or chromatographic process. For example,
the solution can be forced through a porous polymer column, disk or
plug, or the solution can be stirred with the porous material, such
as in a batch-stirred reactor. The solution can also be added to a
porous material-containing well of a microtiter plate. The porous
material can take the form of a monolith or particle, e.g., beads
or pellets. The solution is contacted with the porous material for
a time period sufficient for the solute of interest to
substantially sorb onto the porous material. This period is
typically the time necessary for the solute to equilibrate between
the porous material surface and the solution. The sorption or
partition of the solute onto the porous material can be partial or
complete.
[0107] The invention also includes a method for analytically
determining the level of solute in a solution. A solution having a
solute is contacted with a porous material under conditions so as
to allow sorption of the solute to the porous material. The
material comprises at least one ion-exchange functional group, at
least one hydrophilic polar component and at least one hydrophobic
component. The porous material having the sorbed solute is washed
with a solvent under conditions so as to desorb the solute from the
porous material. The level of the desorbed solute present in the
solvent after the washing is analytically determined.
[0108] The solution contacted with the porous material can comprise
the solute of interest in dilute form, e.g., at a concentration too
low for accurate quantitation. By sorbing the solute onto the
porous material and then, e.g., desorbing the solute with a
substantially smaller volume of a less polar solvent, a solution
which includes the solute of interest can be prepared having a
substantially higher concentration of the solute of interest than
that of the original solution. The method can also result in
solvent exchange, that is, the solute is removed from a first
solvent and re-dissolved in a second solvent.
[0109] Solvents which are suitable for desorbing the solute from
the porous material can be, e.g., polar water-miscible organic
solvents, such as alcohols, e.g., methanol, ethanol or isopropanol,
acetonitrile, acetone, and tetrahydrofuran, or mixtures of water
and these solvents. The desorbing solvent can also be, e.g., a
nonpolar or moderately polar water-immiscible solvent such as
dichloromethane, diethylether, chloroform, or ethylacetate.
Mixtures of these solvents are also suitable. Preferred solvents or
solvent mixtures must be determined for each individual case. A
suitable solvent can be determined by one of ordinary skill in the
art without undue experimentation, as is routinely done in
chromatographic methods development (see, e.g., McDonald and
Bouvier, eds., Solid Phase Extraction Applications Guide and
Bibliography, "A Resource for Sample Preparation Methods
Development," 6th edition, Waters, Milford, Mass. (1995); Snyder
and Kirkland, Introduction to Modern Liquid Chromatography, New
York: J. Wiley and Sons (1974)).
[0110] The level of the desorbed solvent present in the solvent can
be analytically determined by a variety of techniques known to
those skilled in the art, e.g., high performance liquid
chromatography, gas chromatography, gas chromatography/mass
spectrometry, or immunoassay.
[0111] The invention also provides separation devices comprising
the porous materials of the invention. Such devices include
chromatographic columns, cartridges, thin layer chromatographic
plates, filtration membranes, sample clean up devices, solid phase
organic synthesis supports, and microtiter plates. In certain
embodiments, more than one type of functionalized porous material
can be used in the separation devices, e.g., columns, cartridges,
and the like.
[0112] As noted above, the porous materials of the invention are
especially well suited for solid phase extraction. Thus, the
invention also includes a solid phase extraction cartridge
comprising a porous material of the invention packed inside an
open-ended container. In one embodiment, the porous material is
packed as particles within the open-ended container to form a solid
phase extraction cartridge.
[0113] The container can be, e.g., a cylindrical container or
column which is open at both ends so that the solution can enter
the container through one end, contact the porous material within
the container, and exit the container through the other end. In the
form of porous particles, the porous material can be packed within
the container as small particles, such as beads having a diameter
between about 3 .mu.m and about 500 .mu.m, preferably between about
20 .mu.m and about 200 .mu.m. In certain embodiments, the porous
particles can be packed in the container enmeshed in a porous
membrane.
[0114] The container can be formed of any material which is
compatible, within the time frame of the solid phase extraction
process, with the solutions and solvents to be used in the
procedure. Such materials include glass and various plastics, such
as high density polyethylene and polypropylene. In one embodiment,
the container is cylindrical through most of its length and has a
narrow tip at one end. One example of such a container is a syringe
barrel. The amount of porous material within the container is
limited by the container volume and can range from about 0.001 g to
about 50 kg, and preferably is between about 0.025 g and about 1 g.
The amount of porous material suitable for a given extraction
depends upon the amount of solute to be sorbed, the available
surface area of the porous material and the strength of the
interaction between the solute and the porous material. This amount
can be readily determined by one of ordinary skill in the art. The
cartridge can be a single use cartridge, which is used for the
treatment of a single sample and then discarded, or it can be used
to treat multiple samples.
EXAMPLES
[0115] The present invention may be further illustrated by the
following non-limiting examples. All reagents were used as received
unless otherwise noted. Those skilled in the art will recognize
that equivalents of the following supplies and suppliers exist, and
as such the suppliers listed below are not to be construed as
limiting.
Example 1
[0116] To a 3000 mL flask was added a solution of 5.0 g
hydroxypropylmethylcellulose (Methocel E15, Dow Chemical Co.,
Midland, Mich.) in 1000 mL water. To this was added a solution of
175 g divinylbenzene (DVB HP-80, Dow), 102 g N-vinylpyrrolidone
(International Specialty Products, Wayne, N.J.), and 185 g
azobisisobutyronitrile (VAZO 64, Dupont Chemical Co., Wilmington,
Del.) in 242 g toluene (J.T. Baker, Phillipsburgh, N.J.). The 80%
purity divinylbenzene above may be substituted with other
hydrophobic monomers such as styrene or ethylvinylbenzene, or lower
purity grades of divinylbenzene, but 80% purity divinylbenzene is
preferred. The divinylbenzene is stripped with a sodium hydroxide
solution prior to use in the normal way. The N-vinylpyrrolidone
above may be substituted with other hydrophilic monomers such as
N-vinylacetamide, N-vinylpyridine, methacrylate, methyl
methacrylate, vinyl acetate, acrylamide, methacrylamide, but
N-vinylpyrrolidone is most preferred.
[0117] The resulting biphasic mixture was stirred for 30 minutes at
room temperature using sufficient agitation to form oil droplets of
the desired micron size. The resulting suspension was then heated
under moderate agitation at 70.degree. C. and maintained at this
temperature for 20 hours. The suspension was cooled to room
temperature, filtered, and washed with methanol. The filter cake
was then dried in vacuo for 16 hours at 80.degree. C. The
composition of the product polymer was determined by combustion
analysis (CE-440 Elemental Analyzer; Exeter Analytical Inc., North
Chelmsford, Mass., or equivalent). Elemental analysis N 2.24%; mole
percent N-vinylpyrrolidone: 20%. A series of
poly(divinylbenzene-co-N-vinylpyrrolidone) copolymers comprising
about 13, 14, 16, and 22 mole % N-vinylpyrrolidone was also
prepared by this method varying the starting ratio of the
divinylbenzene and N-vinylpyrrolidone monomers.
Example 2
[0118] Poly(divinylbenzene-co-N-vinylpyrrolidone), OASIS.RTM. HLB,
obtained from Waters Corp., Milford, Mass., was derivatized with
hydrochloric acid (12 Molar, 36.5-38%, A.C.S. reagent, J.T. Baker,
9535-03, Phillipsburgh, N.J.) and paraformaldehyde (95%, Aldrich
Chemical, 15,812-7, Milwaukee, Wis.). A three-necked, round-bottom
flask was fitted with a thermometer, agitator, condenser and
reactor temperature control system. Hydrochloric acid was
introduced into the flask. In some cases, water was added to the
flask prior to hydrochloric acid addition in order to dilute the
acid concentration to below 12 M. Then, the agitation and the
temperature control were started. The agitator was a ground-glass
shaft fitted through the proper Teflon bearing into the center
opening atop the flask. The Teflon paddle was single-bladed. The
agitation rate was adjusted to ensure adequate mixing. The
poly(divinylbenzene-co-N-vinylpyrrolidone), OASIS.RTM. HLB, was
charged. Next, the paraformaldehyde was charged. The reaction
mixture was stirred for a certain period of time at constant
temperature. The reaction mixture was cooled, and the acid solution
was filtered. The chloromethylated
poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer was collected
and washed with water until the pH of the slurry was .gtoreq.5.0.
In the case of Examples 2s and 2u, the copolymer was washed with
water until the pH was .gtoreq.3, and then a requisite amount of
concentrated ammonium hydroxide was added to bring the pH between 8
and 9. The materials were then water washed until the pH was
.about.7. The filter cake of chloromethylated
poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer was then
washed twice with methanol (HPLC grade, J.T. Baker, 9535-03,
Phillipsburgh, N.J.) and dried in vacuo for 15 hours at 80.degree.
C. In the case of Examples 2t and 2u, the copolymer was dried
directly from the water wet state with no methanol wash. The level
of chloromethylation was determined by chlorine elemental analysis
(Atlantic Microlab Inc., Norcross, Ga.). Reagent amounts, reaction
conditions, and the resultant loading of chloromethyl groups
(CH.sub.2Cl) are listed in Table 1.
TABLE-US-00001 TABLE 1 Reaction Oasis .RTM. Paraform- Chloromethyl
Temperature Reaction HCl HLB HCl aldehyde Loading Product (.degree.
C.) Time (h) Molarity (g) (g) (g) (meq/g) 2a 50 1 11.1 30 450 17
0.61 2b 60 16 7.5 25 385 4.5 0.72 2c 40 2 12.0 16 225 17 0.73 2d 50
2 11.1 30 450 17 0.74 2e 50 2 12.0 16 225 15 0.83 2f 50 6 11.1 30
450 17 0.89 2g 50 16 11.1 30 450 17 1.00 2h 60 16 9.0 25 385 14.5
1.01 2i 70 2 11.1 30 450 17 1.03 2j 60 5 12.0 16 250 8 1.14 2k 60
16 10.5 25 385 14.5 1.15 2l 70 16 1.1 30 450 17 1.23 2m 70 6 11.1
30 450 17 1.24 2n 60 25 12.0 16 250 8 1.35 2o 65 21 12.0 5 150 8
1.38 2p 70 25 12.0 61 926 51 1.43 2q 65 24 12.0 500 9000 290 0.93
2r 60 24 12.0 100 1500 58 1.09 2s Same batch as 2r (10 g sample),
but with ammonium hydroxide addition. 0.95 2t 65 24 12.0 40 600
23.2 1.20 2u Same batch as 2t (20 g sample) but with ammonium
hydroxide addition 1.15
Example 3
[0119] Chloromethylated poly(divinylbenzene-co-N-vinylpyrrolidone)
porous materials, prepared as described in Example 2, were reacted
with the following amines (all purchased from Aldrich Chemical,
Milwaukee, Wis.): Piperidine (PP), piperazine (PZ),
N-methylpiperazine (MPZ), N-methylbenzylamine (MBZ) and morpholine
(M). A general procedure is provided below. Reagent amounts,
reaction conditions, and the resultant loading of amine groups are
listed in Table 2.
[0120] A 250 mL, three-necked, round-bottom flask was fitted with a
thermometer, agitator, condenser and reactor temperature control
system. The amine was introduced into the flask, and the agitation
and the temperature control were started. In the case of Examples
3b and 3g, the reaction suspension also included a charge of water.
The agitator was a ground-glass shaft fitted through the proper
Teflon bearing into the center opening atop the flask. The Teflon
paddle was single-bladed. The chloromethylated
poly(divinylbenzene-co-N-vinylpyrrolidone) was charged, and the
agitation rate was adjusted to ensure adequate mixing. The reaction
mixture was stirred for a certain period of time at constant
temperature. The reaction mixture was cooled, and the amine was
filtered. The aminated poly(divinylbenzene-co-N-vinylpyrrolidone)
copolymer was collected and washed with water until the pH of the
slurry was .ltoreq.7.0. The filter cake of aminated
poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer was then
washed twice with methanol (HPLC grade, J.T. Baker, 9535-03,
Phillipsburgh, NJ) and dried in vacuo for 15 hours at 80.degree. C.
The loading of amine was determined by fully converting the amine
to its hydrochloride salt with dilute hydrochloric acid, displace
the chloride of the salt by exposing the material to dilute nitric
acid, and then titrating the displaced chloride in solution with
AgNO.sub.3 (Metrohm 716 DMS Titrino autotitrator with silver
electrode, Metrohm, Hersau, Switzerland, or equivalent).
TABLE-US-00002 TABLE 2 Chloro- Chloro- Amine Reaction methyl methyl
Amine Group Temperature Time product load Amine Amount Loading
Product (.degree. C.) (g) (g) (meq/g) Type (g or mL) (meq/g) 3a 105
18 73 1.10 PP 730 g 0.37 3b 100 18 15 0.69 PZ 12.9 g in 0.36 150 mL
water 3c 110 18 15 0.69 MPZ 150 0.43 3d 110 18 15 0.69 M 150 0.16
3e 110 18 20 1.24 MBZ 200 0.2 3f 110 18 20 0.93 M 135 0.18 3g 100
18 20 0.93 M 17.5 mL in 0.17 100 mL water
Example 4
[0121] This example illustrates the preparation of
poly(divinylbenzene-co-N-vinylpyrrolidone) and
poly(divinylbenzene-co-N-vinylpyrrolidone-co-vinylbenzylchloride)
monolithic copolymers. A general procedure is provided below.
Reagent amounts, reaction conditions, and characterization data are
listed in Table 3.
[0122] To a 20 mL glass vial was added N-vinylpyrrolidone (NVP,
Aldrich Chemical, Milwaukee, Wis.), divinylbenzene (DVB HP-80,
Dow), cyclohexanol (CH, Aldrich Chemical, Milwaukee, Wis.),
dodecanol (DD, Aldrich Chemical, Milwaukee, Wis.) and
azobisisobutyronitrile (VAZO 64, Dupont Chemical Co., Wilmington,
Del.). In some cases Vinylbenzyl chloride was also added (VBC,
Fluka, Milwaukee, Wis.). These mixtures were mixed and purged with
nitrogen for 5 minutes before 5 mL aliquots were transferred to
individual 10 mL glass vials. Each of these vials was placed in an
oven at 75.degree. C. for 24 hours. Following removal from the
vials, each monolith was placed in refluxing methanol for 32 hours
and then dried for 24 hours at 75.degree. C. and an additional 24
hours at 85.degree. C. under vacuum. The level of chloromethylation
was determined by chlorine elemental analysis (Atlantic Microlab
Inc., Norcross, Ga.). Reagent amounts, % C, porosity data, and the
resultant loading of chloromethyl groups (CH.sub.2Cl) are listed in
Table 3.
[0123] The % C was determined as in Example 1. The resultant
loading of chloromethyl groups (CH.sub.2Cl) was determined as in
Example 2. The specific surface areas (SSA), specific pore volumes
(SPV) and the average pore diameters (APD) of these materials were
measured using the multi-point N.sub.2 sorption method
(Micromeritics ASAP 2400; Micromeritics Instruments Inc., Norcross,
Ga., or equivalent). The specific surface area was calculated using
the BET method, the specific pore volume was the single point value
determined for P/P.sub.0>0.98, and the average pore diameter was
calculated from the desorption leg of the isotherm using the BJH
method. The macropore pore volume (MPV) of the resultant materials
was measured by Mercury Porosimetry (Micromeritics AutoPore II 9220
or AutoPore IV Micromeritics, Norcross, Ga., or equivalent).
TABLE-US-00003 TABLE 3 NVP DVB CH DD AIBN VBC SSA TPV APD Cl MPV
Product (g) (g) (g) (g) (g) (g) % C (m.sup.2/g) (cm.sup.3/g)
(.ANG.) (meq/g) (mL/g) 4a 2.2 3.9 10.8 1.2 0.08 0 81.9 394 0.72 120
0 0.54 4b 2.2 3.3 10.8 1.2 0.08 0.76 87.3 657 1.31 117 0.92 1.42 4c
2.0 3.5 10.8 1.2 0.08 0.76 87.1 653 1.25 112 0.89 1.48
Example 5
[0124] Monoliths of the type 4b were placed in 100 mL round bottom
flasks equipped with reflux condensers, containing 50 mL of either
methylpiperazine (MPZ), N-methylbenzylamine (MBZ) (Aldrich
Chemical, Milwaukee, Wis.). The flasks were heated for 16 hours, at
which point, they were cooled and the monoliths removed. The
monoliths were then placed in refluxing methanol for 24 hours
(replaced with fresh methanol after 12 hours), before being dried
for 24 hours at 85.degree. C. under vacuum. Amine type, reaction
temperature, and the resultant loading of amine groups are listed
in Table 4. The % C and % N were determined as in Example 1. The
loading of amine was determined as described in Example 3.
TABLE-US-00004 TABLE 4 Temperature Amine Load Product Amine
(.degree. C.) % C % N (meq/g) 5a MPZ 110 86.6 2.75 0.36 5b MBZ 140
89.5 1.74 0.14
Example 6
[0125] Products of Example 3b and 3f were placed in 2.1.times.20 mm
chromatographic columns were using a slurry packing technique. The
packed columns were subsequently employed in an modular valve
switching system comprising a 2777 Sample Manager, a 1525.mu.
Binary HPLC pump, a 2-Position, 6-Port Solvent Selector Valve (two
total), a 515 HPLC pump, (two total), a Quattro Ultima Pt detector,
and MassLynx 4.0 software (all from Waters Corporation, Milford,
Mass., or equivalent). An 80 .mu.L injection of dipyrone
(Sigma-Aldrich, Milwaukee, Wis.) in a water solution (10 ng/mL) was
loaded onto the column in a water mobile phase containing 3% formic
acid for 1 minute at 4 mL/min. The column was then washed with a
methanol solution containing 3% formic acid for 1 minute at 4
mL/min. The dipyrone was then eluted from the column with a 95:5
(v/v) methanol:3% NH.sub.4OH water mobile phase at 0.4 mL/min.
FIGS. 1 and 2 show the total ion chromatograms (TIC) for the
elution of dipyrone using the products of Example 3b and 3f
respectively.
Example 7
[0126] Products of Example 3b and 3f were placed into 1 cc
cartridges (30 mg/cartridge) using a dry packing technique. Each
cartridge was conditioned with 1000 .mu.L methanol and then
conditioned with 1000 .mu.L water. A 1000 .mu.L sample was loaded
which consisted of isotonic saline spiked with the following
analytes: 2-naphthalenesulfonic acid (2.5 .mu.g), amitriptyline (5
.mu.g), ketoprofen (15 .mu.g), salicylic acid (7.5 .mu.g),
secobarbital (12.5), 4-propylbenzoic acid (15 .mu.g). Each loaded
cartridge was washed with 1000 .mu.L 25 mM sodium acetate, pH=4
(designated Fraction 1), and then washed with 1000 .mu.L of
methanol (designated Fraction 2). Next each cartridge was eluted
with 1000 .mu.L of 2% ammonium hydroxide in 20:80 (v:v)
methanol-acetonitrile (designated Fraction 3) and then eluted again
with 500 .mu.L of 2% ammonium hydroxide in 20:80 (v:v)
methanol-acetonitrile (designated Fraction 4). All fractions after
the equilibration were collected, and 500 .mu.L of 0.1 mg/mL butyl
paraben in acetonitrile was added as an internal standard. Fraction
1 was then diluted with 1000 .mu.L of 2% formic acid in 20:80 (v:v)
methanol-acetonitrile. Fractions 2 and 3 were then diluted with
1000 .mu.L of saline. Fraction 4 was diluted with 500 .mu.L of
saline. All fractions were diluted with 100 .mu.L of concentrated
phosphoric acid prior to injection. All analytes were from
Sigma-Aldrich (Milwaukee, Wis.), all reagents were from J.T. Baker
(Phillipsburgh, N.J.).
[0127] Each fraction was measured for sample recovery using the
following HPLC equipment and method (all from Waters Corporation,
Milford, Mass. or equivalent): Waters 600 HPLC pump; Waters 717
autosampler; Waters 486 UV detector; 3.5 .mu.m SymmetryShield.TM.
RP.sub.8 column, 4.6.times.75 mm; Mobile phase 68:32 (v:v) 20 mM
K.sub.2HPO.sub.4, pH 2.7--acetonitrile; Flow rate was 2.0 mL/min;
Temperature 35.degree. C. Detection UV at 214 nm. Injection volume
15 .mu.L. Percent recoveries and the corresponding % RSD are listed
in Table 5 for the average of two runs.
TABLE-US-00005 TABLE 5 % Analyte Recovery Prod- Frac- Frac- Frac-
Frac- % uct Analyte tion 1 tion 2 tion 3 tion 4 RSD 3b
2-naphthalenesulfonic 0.0 0.0 97.6 0.0 0.05 acid amitriptyline 0.0
93.3 0.0 0.0 0.53 ketoprofen 0.0 0.0 96.3 0.0 0.18 salicylic acid
0.0 0.0 96.7 0.0 0.25 secobarbital 0.0 94.0 0.0 0.0 0.08
propylbenzoic acid 0.0 0.0 95.2 0.0 0.01 3f.sup.
2-naphthalenesulfonic 0.0 0.0 92.5 1.1 2.33 acid amitriptyline 0.0
93.5 0.0 0.0 1.86 ketoprofen 0.0 91.8 2.8 0.0 1.45 salicylic acid
0.0 4.3 88.4 0.0 0.97 secobarbital 0.0 94.7 0.0 0.0 1.27
propylbenzoic acid 0.0 96.7 0.0 0.0 1.04
INCORPORATION BY REFERENCE
[0128] The entire contents of all patents, published patent
applications and other references cited herein are hereby expressly
incorporated herein in their entireties by reference.
EQUIVALENTS
[0129] Those skilled in the art will recognize, or be able to
ascertain using no more than routine experimentation, numerous
equivalents to the specific procedures described herein. Such
equivalents were considered to be within the scope of this
invention and are covered by the following claims. The contents of
all references, issued patents, and published patent applications
cited throughout this application are hereby incorporated by
reference.
* * * * *