U.S. patent application number 13/505788 was filed with the patent office on 2012-09-06 for antifungal mixture with fungal organism pythium oligandrum.
This patent application is currently assigned to BIO AGENS RESEARCH AND DEVELOPMENT - BARD, s.r.o.. Invention is credited to Radim Klimes, Martin Suchanek.
Application Number | 20120225035 13/505788 |
Document ID | / |
Family ID | 42289467 |
Filed Date | 2012-09-06 |
United States Patent
Application |
20120225035 |
Kind Code |
A1 |
Suchanek; Martin ; et
al. |
September 6, 2012 |
ANTIFUNGAL MIXTURE WITH FUNGAL ORGANISM PYTHIUM OLIGANDRUM
Abstract
The antifungal mixture is designed for fighting human diseases
and animal diseases of fungal, bacterial or other origin and for
violation of bio-films on heterogeneous materials used both in
human and veterinary medicine and for elimination of microflora
from various objects coming in contact with humans or animals. The
antifungal mixture uses the active component of the fungal organism
Pvthium oligandrum in the mixture with inert components; the said
antifungal mixture contains 0.001 to 25 weight portions of the
fungal organism Pythium oligandrum and 75 to 99.999 weight portions
of inert components. The activity of the fungal organism Pythium
oligandrum in the mixture arises at the moment when this organism
gets in touch with humidity.
Inventors: |
Suchanek; Martin; ( Praha 6,
CZ) ; Klimes; Radim; (US) |
Assignee: |
BIO AGENS RESEARCH AND DEVELOPMENT
- BARD, s.r.o.
|
Family ID: |
42289467 |
Appl. No.: |
13/505788 |
Filed: |
December 14, 2009 |
PCT Filed: |
December 14, 2009 |
PCT NO: |
PCT/CZ09/00154 |
371 Date: |
May 3, 2012 |
Current U.S.
Class: |
424/93.1 |
Current CPC
Class: |
A61K 36/06 20130101;
A61P 31/10 20180101; A61P 1/00 20180101; A61P 31/04 20180101; A61P
17/00 20180101; A01N 63/30 20200101; A01N 63/00 20130101; A61P 3/00
20180101 |
Class at
Publication: |
424/93.1 |
International
Class: |
A61K 35/66 20060101
A61K035/66; A61P 31/10 20060101 A61P031/10 |
Foreign Application Data
Date |
Code |
Application Number |
Nov 4, 2009 |
CZ |
PV 2009-724 |
Claims
1. The antifungal mixture with fungal organism Pythium oligandrum
for fighting human diseases of fungal, bacterial or other origin on
skin, nails, mucous membranes, in the hair part or on the body of
humans, animal diseases of fungal, bacterial or other origin on
paws, cloven hoofs, hoofs, hair, skin, eyes, shells, in mouth,
scales, mucous membranes and auditory passages of animals or in the
body of animals and for violation of bio-films on heterogeneous
materials used in both human and veterinary medicine and for the
elimination of microflora from clothing, its accessories, bed linen
and from walls and other building materials and for elimination of
microflora from objects getting in contact with humans and animals
characterized by the fact that it consists of 0.001 to 25 weight
portions of the fungal organism Pythium oligandrum, when the number
of the reproduction organs of the fungal organism Pythium
oligandrum is higher than 2.times.10.sup.5 in 1 g of the mixture,
and 75 to 99.999 weight portions of the inert components.
2. The antifungal mixture with fungal organism Pythium oligandrum
according to the claim 1, characterized by the fact that the inert
components are inorganic and/or organic solutions and/or
suspensions, and/or emulsions and/or solid substances.
Description
FIELD OF THE INVENTION
[0001] The invention relates to an antifungal mixture with fungal
organism Pythium oligandrum designed for fighting fungi including
fungal diseases and bacteria.
BACKGROUND OF THE INVENTION
[0002] Fungi and fungal diseases are a problem of every society and
especially fungal diseases of skin of both people and animals
belong to frequently occurring diseases of people and animals. Such
fungal diseases are even more serious because they are usually
transmitted not only by a direct contact of the sick and the
healthy but also through the objects, which were in contact with
the source of infection.
[0003] The treatment of such dermatological diseases is very
difficult and lengthy and nowadays, the treatment methods are based
especially on the application of system antimycotics, exceptionally
also chemotherapy, which means that aggressive forms of treatment
with chemical therapeutics are used against pathogenic
microorganisms, which cause the diseases; however, they usually
impact not only the targeted pathogen but also the tissues of the
recipient of the therapeutics. Other aggressive forms of treatment
with antibiotics and hormones have contraindication, too.
[0004] The said conservative types of treatment are often completed
with cosmetic preparations, which are, however, based on the
application of chemistry again, even though there are also natural
cosmetic preparations, the basic source of which are substances of
both floral and animal origin, including some inorganic substances
occurring in nature (salts etc.).
[0005] Only recently, dermatological troubles have started to be
approached differentially and in a complex way and sensitive
natural methods have been used preferentially more and more. Such
methods also include e.g. the application of the fungus Pythium
oligandrum according to the document CZ 9883 U1 in the form of the
biological preparation for fighting the origins of dermatophysis,
which includes the said fungal organism in the form of oospora as
the effective substance, when the total number of oospora is
2.times.10.sup.5 in 1 g of the preparation as a maximum. However,
this solution has a limited range of application and its effective
substance is only bound on the inorganic carrier, which is silicon
dioxide.
[0006] Fungi from walls and fungi, yeast fungi and bacteria from
various objects are removed by means of a series of different
preparations, which are, however, never based on the application of
the effective substance of the fungal organism Pythium
oligandrum.
SUMMARY OF THE INVENTION
[0007] The above mentioned disadvantages of the current solutions
are eliminated to a certain extent with the antifungal mixture with
the fungal organism Pythium oligandrum according to the invention,
where the effective substance of the fungal organism is used in
higher concentrations and with the application of new components of
the final mixtures and newly also zoospore and zoosporangia and
enzymes.
[0008] The fungal organism Pythium oligandrum from the kingdom
Chromista--Stramenopila is a strong mycoparasite; it parasites over
20 families of causative agents of fungal diseases and represents
the dividing line between fungi and protozoa because of its sexual
as well as asexual reproduction. The fibres of Pythium oligandrum
penetrate the cells of the parasite (fungi, yeast fungi, bacteria)
and draw the substances required for its own nutrition from them.
On the basis of nutrition and spatial competition, it squeezes out
the pathogens from the spaces. The nature of the activity of this
fungus is, therefore, parasiting on fungi with the simultaneous
production of enzymes, which further increase the efficiency of the
fungus. It can be said that Pythium oligandrum attacks the opponent
with direct parasitism. After the achievement of the task, i.e.
exhaustion of the parasite, Pythium oligandrum disappears from that
locality (both human and animal bodies do not represent a natural
environment for it and it cannot adapt to such environment) and it
gives way for the re-settlement of the so-called normal micro-flora
or, unless it finds new nutrition (fungi), it is encapsulated into
dormancy conditions and its life cycle is paused.
[0009] It has been newly detected that for suppressing fungal
diseases and their symptoms, it is more advantageous to use higher
concentrations of the effective substance, i.e. reproduction
organs, than 2.times.10.sup.5 in 1 g of the mixture. Further to
that, Pythium oligandrum can be used independently or in
combination with other carriers (components) than silicon dioxide,
which is also used according to the aforesaid applied design.
[0010] Higher concentrations of the effective substance can be
achieved by the application of other reproduction organs of the
fungal organism Pythium oligandrum than only oospore. The number of
the reproduction organs of the fungal organism Pythium oligandrum
according to this technical solution is higher than
2.times.10.sup.5 in 1 g of the mixture. In terms of the weight
expression, it means that the mixture consists of 0.001 to 25
weight portions of Pythia oligandrum as a minimum and 75 to 99.999
weights portions of inert components.
[0011] A new finding also concerns other mechanisms of the effect.
Many diseases are caused by the presence of bacteria. With its
metabolism, Pythium oligandrum produces various enzymes used for
the decomposition of host organisms. Such enzymes also represent
unfavourable environment for bacteria and, at the same time, the
presence of bacteria in the affected organs is supported by the
occurrence of microscopic fungi producing nutrition components that
are important to the bacterial development. Pythium oligandrum can
parasite on such microscopic fungi and, thereby violate the
environment suitable for the propagation of pathogenic bacteria.
The combination of both factors results in the suppression of
reproduction of bacteria, which cease in causing the putrefactive
processes. In the same way, the bio-films are violated, which could
cause diseases of the urinary system and, in case of occurrence on
teeth, gingivitis or contaminate heterogeneous materials used in
various treatment methods.
[0012] Surprisingly it was detected that Pythium oligandrum also
suppresses symptoms of other diseases than dermatophysis, namely
also microbiological settlement of proboscis, other inflammations
of buccal caxity, psoriasis, haemorrhoids, varicose ulcers, bed
sores, "diabetic feet", atopic eczema, acne, cold sores, sore
spots, nail fungi, falling hair, dandruff or old-age itchiness. In
the veterinary section, this solution can be newly used for
suppressing mycotic infections and putrefactive processes on paws,
cloven hoofs, hoofs, hair, skin, eyes, shells, in mouth, scales,
mucous membranes and auditory passages of animals or in the body of
animals.
[0013] Therefore, this new solution enables to use the mixture for
the treatment of human diseases of fungal, bacterial or other
origin on skin, mucous membranes, in the hair part or in the body
of humans. Further to that, this solution enables to use the
mixtures for animals against the diseases of fungal, bacterial or
other origin on paws, cloven hoofs, hoofs, hair, skin, eyes,
shells, in mouth, scales, mucous membranes and auditory passages of
animals or in the body of animals.
[0014] Another application is also the violation of bio-films on
heterogeneous materials used both in human and veterinary medicine
(artificial valves, joint replacements, contact lenses, catheters
etc.). The technical solution also enables to eliminate microflora
virtually from all objects, which come into contact with humans and
animals, e.g. from interiors of the means of transport, seat
furniture, animal cages etc.
[0015] The above mentioned new applications of the fungal organism
Pythium oligandrum are also enabled by the new application of other
components forming the mixture. New mixtures were examined and
selected because of better, more understandable and more
comfortable application of the preparation for the consumer so that
their possible preparation for application and the actual
application were clear, simple and comfortable and corresponded to
the common habits in the twenty first century. At the same time,
they shall also comply with the requirements for the purpose of
application. Various inert carriers are used for the new mixtures
in different ratios, which do not damage the microscopic fungus
Pythium oligandrum (creation of the fungal fibres and
characteristic echinate oospore, zoospore and zoosporangia) and
producing various enzymes, which are collectively called
oligandrin.
[0016] Pythium oligandrum is used in various concentrations for
these new mixtures, when the number of the reproductive organs of
the fungal organism Pythium oligandrum is higher than
2.times.10.sup.5 in 1 g of the mixture.
[0017] The new inert components are especially biological organic
and/or inorganic solutions, such as oil bases, physiological
solutions, aromatic solutions, ointments, suppositories, pastes,
creams, toothpastes, mouthwashes etc. Further to that, it concerns
suspensions, emulsions or solid (loose) compositions of individual
components, e.g. dusting powders, powders etc. or suspensions,
emulsions or solutions from such individual substances. In terms of
weight expression, as stated above, it means that the mixture
includes 0.001 to 25 weight portions of Pythium oligandrum and 75
to 99.999 weight portions of inert components.
[0018] The activity of Pythium oligandrum in the mixture only
arises after soaking or wetting e.g. also by skin, which is always
sweating etc.
EXAMPLES
[0019] (In All Cases, it Concerns % Representation of Weight
Portions)
Example No. 1
Mixture for Suppressing Aphthae and Parodontosis
TABLE-US-00001 [0020] Pythium oligandrum 10.1% Inert component
89.9% of which Citric acid 33.3% Sodium carbonate 2.6% Spearmint
aroma 0.45% Sorbitol 23.45% Sodium acid carbonate 26.79%
Polyethylene glycol 6000 3.34%
Example No. 2
Mixture for Suppressing Atopic Eczema
TABLE-US-00002 [0021] Pythium oligandrum 0.5% Inert component 99.5%
of which Residue of dried organic cultivation medium approx. 9.5%
Refined olive oil 85.09% Colloid silicon dioxide (Aerosil 200) 4.8%
Vitamin E (Covi ox T 70) 0.08% Eucalyptus oil 0.03%
Example No. 3
Mixture for Softening Dry Skin in Case of Diabetes
TABLE-US-00003 [0022] Pythium oligandrum 1.72% Inert component
98.28% of which Residue of dried organic cultivation medium approx.
30.5% Refined almond oil 61.37% Aerosol 200 6.30% Sea buckthorn oil
1.65% Vitamin E (Covi ox T 70) 0.08% Balm oil 0.03%
Example No. 4
Mixture for Suppressing Symptoms of Psoriasis
TABLE-US-00004 [0023] Pythium oligandrum 0.25-0.5% Inert component
99.75-99.5% of which Residue of dried organic 4.75-9.5% cultivation
medium approx. Shea butter BIO (BIO .sup. 34.5% Butyrospermum
Partii Butter) Sunflower oil BIO (BIO .sup. 34.5% Helianthus Annuus
Seed Oil) Beeswax (Cera Alba) .sup. 11% Silica sand (Oxidum
silicii) 10-15%
Example No. 5
Mixture for Suppressing Microbial Settlement of Auditory Passage of
Animals
TABLE-US-00005 [0024] Pythium oligandrum 25.0% Inert component
75.0% of which Refined olive oil 27.5% Shea butter (Cetiol SB 45)
22.0% Fish oil with vitamin E 18.5% Glycerol monostearate (Cutina
GMS V) 2.6% Sea buckthorn oil 3.3% Covi ox T 70 1.1%
Field of Application
[0025] The antifungal mixture with fungal organism Pythium
oligandrum can be used both in human and veterinary medicine, in
the cosmetic industry and also in households for maintaining
cleanness and for elimination of fungi from walls of building
objects and for violation of bio-films on heterogeneous materials
and for elimination of microflora on objects coming in contact with
humans or animals.
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