U.S. patent application number 13/366192 was filed with the patent office on 2012-08-30 for dna methylation markers associated with the cpg island methylator phenotype (cimp) in human colorectal cancer.
This patent application is currently assigned to University of Southern California. Invention is credited to Mihaela Campan, Peter W. Laird, Tiffany I. Long, Kimberly D. Siegmund, Daniel J. Weisenberger.
Application Number | 20120219946 13/366192 |
Document ID | / |
Family ID | 37308704 |
Filed Date | 2012-08-30 |
United States Patent
Application |
20120219946 |
Kind Code |
A1 |
Laird; Peter W. ; et
al. |
August 30, 2012 |
DNA METHYLATION MARKERS ASSOCIATED WITH THE CPG ISLAND METHYLATOR
PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER
Abstract
Particular aspects confirm the existence of a CpG island
methylator phenotype (CIMP) in colorectal cancer, and provide novel
validated DNA methylation markers associated with CIMP. Additional
aspects provide novel methods and compositions for: determining
CIMP status in colorectal cancers, determining the relationship
between CIMP status and other molecular features of the cancers
(e.g., BRAF mutation, KRAS mutation and MSI status); determining
the relationship between CIMP status and other variables (e.g.,
age, sex, tumor location, family history, race, country of origin,
tumor characteristics (including, tumor type, tumor grade, invasive
margin characteristics, lymphocyte infiltration characteristics,
direct spread, lymph node spread, venous spread and type of
residual adjacent polyp, if present)); and determining, between
subgroups defined by CIMP status and BRAF mutations, effects of
selected risk factors (e.g., body mass index, smoking history,
alcohol intake, dietary folate intake, folate metabolic enzyme
polymorphisms and history of hormonal use).
Inventors: |
Laird; Peter W.; (South
Pasadena, CA) ; Siegmund; Kimberly D.; (San Marino,
CA) ; Campan; Mihaela; (Los Angeles, CA) ;
Weisenberger; Daniel J.; (Playa del Rey, CA) ; Long;
Tiffany I.; (Chino, CA) |
Assignee: |
University of Southern
California
Los Angeles
CA
|
Family ID: |
37308704 |
Appl. No.: |
13/366192 |
Filed: |
February 3, 2012 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
11913535 |
Dec 28, 2007 |
8110361 |
|
|
PCT/US06/17160 |
May 2, 2006 |
|
|
|
13366192 |
|
|
|
|
60677181 |
May 2, 2005 |
|
|
|
Current U.S.
Class: |
435/6.11 ;
436/501; 536/23.1 |
Current CPC
Class: |
C12Q 2600/154 20130101;
C12Q 2600/158 20130101; C12Q 2600/156 20130101; C12Q 2600/112
20130101; C12Q 1/6886 20130101 |
Class at
Publication: |
435/6.11 ;
436/501; 536/23.1 |
International
Class: |
C12Q 1/68 20060101
C12Q001/68; C07H 21/04 20060101 C07H021/04; G01N 33/574 20060101
G01N033/574 |
Goverment Interests
STATEMENT REGARDING FEDERAL SUPPORT
[0002] This work was supported by a NIH grant R01 CA075090, and the
United States Government may have certain rights in this invention.
Claims
1.-21. (canceled)
22. A treated nucleic acid derived from genomic SEQ ID NOS:128-141,
114-127 and 100-113, wherein the treatment is suitable to convert
at least one unmethylated cytosine base of the genomic DNA sequence
to uracil or another base that is detectably dissimilar to cytosine
in terms of hybridization.
23. A nucleic acid, comprising at least 16 contiguous nucleotides
of a treated genomic DNA sequence selected from the group
consisting of SEQ ID NOS:170-197, 226-253, 142-169 and 198-225, and
sequences complementary thereto, wherein the treatment is suitable
to convert at least one unmethylated cytosine base of the genomic
DNA sequence to uracil or another base that is detectably
dissimilar to cytosine in terms of hybridization.
24. A nucleic acid, comprising at least 50 contiguous nucleotides
of a DNA sequence selected from the group consisting of SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225, and sequences
complementary thereto.
25. The nucleic acid of any one of claims 22 to 24, wherein the
contiguous base sequence comprises at least one CpG, TpG or CpA
dinucleotide sequence.
26. A nucleic acid, comprising at least 16 contiguous nucleotides
of nucleic acid sequence selected from the group consisting of SEQ
ID NOS:128-141, 114-127 and 100-113, SEQ ID NOS:170-197, 226-253,
142-169 and 198-225 and sequences complementary thereto as a
diagnostic means.
27. A kit suitable for performing the method according to claim 3
comprising a) a plurality of oligonucleotides or polynucleotides
able to hybridise under stringent or moderately stringent
conditions to the transcription products of at least one gene or
genomic sequence selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (including all transcript variants thereof),
and SEQ ID NOS:128-141, 114-127 and 100-113; (b) a container
suitable for containing the oligonucleotides or polynucleotides and
a biological sample of the patient comprising the transcription
products wherein the oligonucleotides or polynucleotides can
hybridise under stringent or moderately stringent conditions to the
transcription products, (c) means to detect the hybridisation of
(b); and optionally, (d) instructions for use and interpretation of
the kit results.
28. A kit suitable for performing the method according to claim 6,
comprising (a) a means for detecting polypeptides of at least one
gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (including all transcript variants
thereof); (b) a container suitable for containing the said means
and the biological sample of the patient comprising the
polypeptides wherein the means can form complexes with the
polypeptides; (c) a means to detect the complexes of (b).
29. A kit suitable for performing the method according to claim 9,
comprising (a) a bisulfite reagent; (b) a container suitable for
containing the said bisulfite reagent and the biological sample of
the patient; (c) at least one set of oligonucleotides containing
two oligonucleotides whose sequences in each case are identical,
are complementary, or hybridize under stringent or highly stringent
conditions to a 9 or more preferably 18 base long segment of a
sequence selected from SEQ ID NOS:170-197, 226-253, 142-169 and
198-225.
30. A kit suitable for performing the method according to claim 9,
comprising (a) a methylation sensitive restriction enzyme reagent;
(b) a container suitable for containing the said reagent and the
biological sample of the patient; (c) at least one set of
oligonucleotides one or a plurality of nucleic acids or peptide
nucleic acids which are identical, are complementary, or hybridize
under stringent or highly stringent conditions to an at least 9
base long segment of a sequence selected from SEQ ID NOS:128-141,
114-127 and 100-113; and optionally (d) instructions for use and
interpretation of the kit results.
31.-33. (canceled)
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation of U.S. patent
application Ser. No. 11/913,535 filed 28 Dec. 2007 (to issue as
U.S. Pat. No. 8,110,361 on 7 Feb. 2012), which is the national
submission under 35 U.S.C. 371 of International Application
PCT/US2006/017160 filed 2 May 2006, which claims the benefit of
priority to U.S. Provisional Patent Application No. 60/677,181,
filed 2 May 2005, all of which are incorporated by reference herein
in their entireties.
FIELD OF THE INVENTION
[0003] Aspects of the present invention relate generally to cancer
and colorectal cancer, and more particularly to identification of,
and diagnostic and/or prognostic use of novel validated DNA
methylation markers associated with the CpG island methylator
phenotype (CIMP) in colorectal cancer. The present invention also
relates to genomic DNA sequences that exhibit altered expression
patterns in disease states relative to normal. Particular
embodiments provide, inter alia, novel methods, nucleic acids,
nucleic acid arrays and kits useful for detecting, or for detecting
and differentiating CIMP and/or cell proliferative disorders.
Preferably, the methods, nucleic acids, nucleic acid arrays and
kits for the detection and diagnosis of cell proliferative
disorders are used for the diagnosis of CIMP, and in particular
colorectal cancer.
SEQUENCE LISTING
[0004] A Sequence Listing, comprising 335 SEQ ID NOS, has been
provided in computer readable form (.txt) as part of this
application, and is incorporated by reference herein in its
entirety.
BACKGROUND
Cancer Epigenetics
[0005] Epigenetics refers to a switch between phenotypic states
that is not based upon a change in genotype, such as a mutation,
but is the result of a change in gene activity without any
accompanying alteration of the DNA sequence. Simply put, this
amounts to a stable change in gene expression. In cancer
epigenetics, the most commonly observed situation is a change from
a transcriptionally active gene to an epigenetically `silenced`
state. Epigenetic alterations are distinct from transitory changes
in gene regulation, in that they involve relatively extensive,
stable changes in chromatin structure, histone modification,
associated protein composition, and in many cases, altered
distribution of cytosine-5 DNA methylation at CpG dinucleotides in,
for example, the promoter region of the gene. Of all of these
molecular mechanisms, DNA methylation is the easiest to measure in
archival samples, because cytosine methylation is retained in the
genomic DNA throughout, for example, formalin-fixation, years of
storage in paraffin blocks, and subsequent extraction from paraffin
sections. Even heavily degraded, crosslinked DNA can be subjected
successfully to DNA methylation analysis using, for example,
bisulfate-based technique (e.g., with PCR amplicons, as is the case
for the MethyLight.TM. technology).
[0006] The main targets for epigenetic gene silencing in cancer
cells are promoter regions containing G:C- and CpG-rich stretches
of DNA, called `CpG islands.` CpG islands are G:C and CpG-rich
stretches of DNA in the genome, often located in the vicinity of
genes, and generally unmethylated in normal somatic tissues.
Aberrant methylation of CpG islands has, for example, been
documented in both benign and malignant human colorectal tumors and
is associated with gene silencing.
[0007] It is important to note, however, that not all
epigenetically silenced genes in cancer cells are tumor-suppressor
genes, and that many of the affected CpG islands are not even
located in promoter regions, and are not thought to affect gene
expression (e.g., they may be in promoter regions of genes that are
not expressed in either the normal or malignant cells of an organ
giving rise to a malignancy). Yet, the occurrence of CpG island
hypermethylation events is, in many cases, cancer-specific,
suggesting a scenario in which the overall rate of CpG island
hypermethylation during tumorigenesis needs to be sufficiently high
to acquire the necessary hits at key loci, but at the same time
perhaps affecting many other loci which are essentially innocent
bystanders.
[0008] CIMP in Colorectal Cancer; Prior Art Uncertainty and
Inconsistencies.
[0009] A subset of colorectal tumors has been described to have an
unusually high number of hypermethylated CpG islands, leading to
the definition of a distinct phenotype, referred to as "CpG Island
Methylator Phenotype", or "CIMP" (16, 21). Colorectal cancer has a
lifetime incidence of 1 in 20, and CIMP cancers account for at
least 15% of this, representing a large cohort of affected
patients. Colorectal cancer has been traditionally been considered
to be a single disease for the purposes of treatment, but recent
evidence has suggested that there are different outcomes in
sub-groups with distinct molecular features. Such CIMP+ colorectal
tumors have been reported to have distinct profiles of genetic
alterations, anatomic subsite, gender prevalence, histopathologic
characteristics and clinical behavior.
[0010] However, a clear understanding of the CIMP phenomenon has
been hampered by two complexities. First, the intricacy of the
relationship between CIMP and microsatellite instability continues
to result in different perspectives on the issue (38, 77). Second,
the concept that CIMP affects only a subset of colorectal tumors
and a subset of CpG islands, as opposed to all CpG islands known to
be susceptible to hypermethylation, is not universally embraced or
appreciated (38), and is further complicated by the fact that there
are no firm rules for recognizing which CpG islands belong to the
CIMP group. An initial panel of cancer-specifically methylated CpG
island markers (21) has not been applied consistently in the
literature, nor does it appear that all cancer-specifically
methylated genes are affected by CIMP. Nevertheless, it seems clear
that some CpG islands are more prone to cancer-specific
hypermethylation than others. Thus, a lack of standardization in
the classification methods used to define CIMP has resulted in
varying and contradictory conclusions regarding, or example, the
association of CIMP with a family history of cancer, and even the
very existence of CIMP as a distinct subgroup of colorectal tumors.
Not only has there been some ambiguity as to which CpG islands
belong to the CIMP subset, but there has also been a lack of a
uniform standard for applying this subset to the definition of CIMP
status. The existence of unique CIMP-related etiologic and
pathogenetic mechanisms can only be defined when this subgroup can
be clearly and accurately identified. There are presently no clear
guidelines for what constitutes CIMP-associated versus
non-CIMP-associated CpG islands.
[0011] Therefore, there is a pronounced need in the art to further
elucidate and understand the epidemiology and etiology of DNA
methylation alterations in human colorectal cancer, and to clarify
the uncertainties regarding the existence of CIMP and its
classification. There is a pronounced need in the art to not only
unambiguously confirm the existence of CIMP as a distinct subgroup
of colorectal cancer, but also to establish an improved CIMP
classification panel of methylation markers. There is a pronounced
need in the art to provide compositions and methods for determining
the relationship between CIMP status and molecular, demographic,
and histopathologic features, and environmental risk factors. There
is a pronounced in the art to understand the pathogenesis of this
colorectal cancer subset and its association with risk factors so
that we will be better placed to prevent its occurrence. There is a
pronounced in the art to correctly identify cases which will best
respond to particular therapies.
SUMMARY OF THE INVENTION
[0012] Applicants, as described herein, have provided definitive
evidence for the existence of CIMP as a distinct trait among
colorectal adenocarcinomas. Additionally, applicants have developed
an improved method for the classification of CIMP. Furthermore,
applicants have found that CIMP underlies almost all cases of
sporadic MSI-H colorectal cancer and tumors with mutation of the
BRAF oncogene.
[0013] Therefore, aspects of the present invention confirm the
existence of a CpG island methylator phenotype (CIMP) in colorectal
cancer, and provide novel validated DNA methylation markers
associated with CIMP.
[0014] Additional aspects provide novel methods and compositions
for determining CIMP status in colorectal cancers.
[0015] Particular aspects provide a panel of 14 CIMP genomic
(preferably, particular CpG islands thereof described herein)
markers corresponding to BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6), SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) and a preferred sub panel of 5 genomic
(preferably, particular CpG islands thereof described herein)
markers (CACNA1G, IGF2, NEUROG1, RUNX3, and SOCS1), that provide,
inter alia, an excellent classifier for CIMP status.
[0016] Additional preferred aspects provide a panel of three
genomic and CpG island markers that identify KRAS mutant, BRAF
wildtype tumors (CDKN2A, CRABP1 and NEUROG1); that is that are
positively associated with KRAS mutation (after exclusion of BRAF
mutant tumors, indicating that a separate KRAS-associated CIMP
subgrouping exists with an overlapping set of methylation
markers).
[0017] Further aspects provide novel methods and compositions for
determining the relationship between CIMP status and other
molecular features of the cancers including, but not limited to
BRAF mutation, KRAS mutation and MSI status.
[0018] Additional aspects provide novel methods and compositions
for determining the relationship between CIMP status and other
variables including, but not limited to age, sex, tumor location,
family history, race, country of origin, tumor characteristics
(including, tumor type, tumor grade, invasive margin
characteristics, lymphocyte infiltration characteristics, direct
spread, lymph node spread, venous spread and type of residual
adjacent polyp, if present).
[0019] Yet additional aspects provide novel methods and
compositions for determining, between subgroups defined by CIMP
status and BRAF mutations, effects of selected risk factors
including, but not limited to body mass index, smoking history,
alcohol intake, dietary folate intake, folate metabolic enzyme
polymorphisms and history of hormonal use.
[0020] Further aspects of the present invention provide a
foundation for a population-based study of CIMP, by providing a
novel panel of very carefully selected methylation markers
representing the CIMP subgroup, and having utility to classify
CIMP.
BRIEF DESCRIPTION OF THE DRAWINGS
[0021] FIG. 1 shows an identification of Type C methylation
markers. MethyLight.TM. analysis was performed for 195
MethyLight.TM. reactions on five CIMP+ and five CIMP- tumors as
described in Methods (Example 1). Prior CIMP classification was
performed as described in the Methods section "Tissue Samples". PMR
values are indicated in color with a PMR of 0 (very light blue),
less than 5 (light yellow), 5-50 (dark yellow), and above 50 (dark
red). Genes selected for further evaluation are grouped at the
bottom and sorted by increasing mean PMR from top to bottom.
[0022] FIGS. 2A and 2B show identification of tumor clusters.
Hierarchical cluster analysis of DNA samples from 48 colorectal
tumor and matched normal mucosae and 92 methylation markers,
performed as described in Methods (Example 1). Clustering of the
cases was based on the tumor methylation data shown in the upper
heatmap (Panel A). The resulting dendrogram of the cases is shown
at the top, with tumors containing KRAS mutation indicated by a red
rectangle overlaying the branch, while BRAF mutations are indicated
by a green rectangle, and MSI-H cases are designated with a blue
rectangle. Clustering of the methylation markers was performed
separately for the matched normal mucosal samples (Panel B). DNA
methylation quartiles are shown from very light blue-white
(lowest), through light yellow (next lowest), dark yellow (third
lowest) to dark red (highest methylation quartile.
[0023] FIGS. 3A and 3B show independent testing of 14 methylation
markers. The best nine CIMP markers and best five Type C markers
were selected based on the data in FIG. 2 as described in Methods
(Example 1) and tested on an independent set of consecutive 187
colorectal tumors. Panel A: Dichotomous heatmap of the DNA
methylation data, with tumors with increasing frequency of DNA
methylation sorted to the bottom. Methylation markers are arranged
alphabetically. Red bars indicate PMR.gtoreq.10, whereas very light
blue bars indicate PMR<10. The optimal threshold for CIMP+ based
on minimizing the within group sum of squared errors was 7 or more
methylated markers out of 14. CIMP status defined as such is
indicated for each tumor in black (CIMP+) or gray (CIMP-) to the
right of the heatmap. Panel B: Histogram showing the distribution
of the numbers of tumors with different numbers of methylated
markers.
[0024] FIGS. 4A-4F show comparison of CIMP panel performance. The
187 tumors shown in FIG. 3 were screened against two sets of CIMP
markers as described in the Methods (Example 1). The data are
represented as in FIG. 3A. The alignment of each tumor is
maintained across panels A, C, and E. Panel A: Dichotomous heatmap
representing DNA Methylation data using a classic set of CIMP
markers. Panel B: Histogram of the methylation frequency
distribution for the set of classic CIMP markers. Panel C:
Dichotomous heatmap representing DNA Methylation data using a newly
developed set of CIMP markers. Panel D: Histogram of the
methylation frequency distribution for the new set of CIMP markers.
For both marker panels, a tumor was considered CIMP+ if at least 3
of 5 markers were methylated. CIMP status determined by each panel
is indicated to the right of each panel heatmap, with light gray
indicating CIMP- and black indicating CIMP+. Panel E: Covariate
status of each tumor is shown on the right, with the presence of
BRAF and KRAS mutations indicated with blue bars and wild-type
alleles indicated in green. Microsatellite instability (MSI) status
is indicated as MSI-high (MSI-H) (blue bars), MSI-low (MSI-L) (very
light blue bars), and microsatellite stable (MSS) (green bars).
Cases consistent with Hereditary Non-Polyposis Colorectal Cancer
(HNPCC) are indicated by blue bars. Female and proximal colonic
location cases are indicated with blue bars, while male and distal
colonic location cases are indicated with green bars. Panel F: Pie
charts illustrating the relative frequencies of BRAF and KRAS
mutations and MSI status categories for CIMP- (Top) and CIMP+
(Bottom) tumors are shown on the lower right, with color codings as
described above.
[0025] FIGS. 5A-5D show a final independent test of CIMP panels.
The classic and new CIMP panels were screened against 50 colorectal
tumors obtained from the Mayo Clinic and Foundation, Rochester,
Minn. These tumors were selected to include 40 MSS cases and 10
MSI-H, non-HNPCC cases to most efficiently compare the associations
of the two panels on a modest number of tumors. Panel A:
Dichotomous heatmap representing DNA Methylation data using a
classic set of CIMP markers. Panel B: Histogram of the methylation
frequency distribution for the set of classic CIMP markers. Panel
C: Dichotomous heatmap representing DNA Methylation data using a
newly developed set of CIMP markers. Panel D: Histogram of the
methylation frequency distribution for the new set of CIMP markers.
For both marker panels, a tumor was considered CIMP+ if at least 3
of 5 markers were methylated. CIMP status determined by each panel,
BRAF mutation status, and MSI status is indicated to the right of
each panel heatmap, with black indicating CIMP+, BRAF mutant, and
MSI-H status and light gray marking CIMP-, BRAF wildtype, and MSS
status.
[0026] FIGS. 6A-6E show, according to particular aspects,
methylation-specific PCR (MSP) of a New CIMP Panel on CIMP+ and
CIMP- colon tumor DNA samples. MSP reactions specific for the
methylated (M) and the unmethylated (U) bisulfate-converted DNA
sequences were designed for each gene in the New CIMP Panel: (A)
CACNA1G, (B) IGF2, (C) NEUROG1, (D) RUNX3 and (E) SOCS1. Each MSP
reaction was evaluated on two CIMP+ and two
CIMP-bisulfate-converted colon tumor DNA samples using AmpliTaq
Gold DNA polymerase (Applied Biosystems). Human sperm DNA and human
sperm DNA treated in vitro with the M.SssI CpG methylase served as
negative and positive methylation controls, respectively, for each
MSP reaction. The PCR products were visualized by UV illumination
after electrophoresis on 3% agarose gels and ethidium bromide
staining. A 100 base pair molecular weight ladder was used to
assess the PCR product size. MSP reactions tend to be less
specific, but more sensitive than MethyLight reactions, since they
lack the additional methylation specificity conferred by the
MethyLight probe. This difference may be particularly noticeable
for the less specific markers in our panel, such as NEUROG1
(highest percentage of methylated tumors in FIGS. 4 and 5). One
CIMP- tumor was methylated for the NEUROG1 marker, consistent with
MethyLight.TM. results obtained for this tumor. The threshold of
three methylated markers out of a panel of five markers for the
definition of CIMP-positive tumors was developed for the equivalent
MethyLight.TM. reactions. The optimal threshold may differ when
using the MSP versions of these markers. The MSP primer and probe
sequences are described in the table of MSP primers on page 3.
DETAILED DESCRIPTION OF THE INVENTION
[0027] Aspects of the present invention confirm the existence of a
CpG island methylator phenotype (CIMP) in colorectal cancer, and
provide novel validated DNA methylation markers associated with
CIMP.
[0028] Additional aspects provide novel methods and compositions
for determining CIMP status in colorectal cancers.
[0029] CIMP is a relatively new subclassification with an unknown
underlying molecular defect. There is currently no external gold
standard for defining CIMP, against which CIMP markers and panels
can be compared and performance evaluated. Particular aspects
disclose and describe a careful and methodical screen, starting
with 195 different CpG islands, through a stepwise selection
process involving the analysis of 245 different colorectal
adenocarcinomas, and employing a battery of different cluster
analysis routines, all of which identified the same subset of CIMP
tumors, to arrive at a panel of 14 preferred CIMP markers (BCL2,
BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1) genomic markers (preferably,
particular CpG islands thereof described herein) (TABLE 6), and a
preferred sub panel of 5 markers (CACNA1G, IGF2, NEUROG1, RUNX3,
and SOCS1) (preferably, particular CpG islands thereof described
herein), that provide an excellent classifier for CIMP status.
[0030] Additional preferred aspects provide a panel of three
genomic markers (CDKN2A, CRABP1 and NEUROG1) (preferably,
particular CpG islands thereof described herein) that identify KRAS
mutant, BRAF wildtype tumors.
[0031] Further aspects provide novel methods and compositions for
determining the relationship between CIMP status and other
molecular features of the cancers including, but not limited to
BRAF mutation, KRAS mutation and MSI status.
[0032] Additional aspects provide novel methods and compositions
for determining the relationship between CIMP status and other
variables including, but not limited to age, sex, tumor location,
family history, race, country of origin, tumor characteristics
(including, tumor type, tumor grade, invasive margin
characteristics, lymphocyte infiltration characteristics, direct
spread, lymph node spread, venous spread and type of residual
adjacent polyp, if present).
[0033] Yet additional aspects provide novel methods and
compositions for determining, between subgroups defined by CIMP
status and BRAF mutations, effects of selected risk factors
including, but not limited to body mass index, smoking history,
alcohol intake, dietary folate intake, folate metabolic enzyme
polymorphisms and history of hormonal use.
TABLE-US-00001 TABLE 6 List of 14 Preferred CIMP markers, along
with representative amplicons and respective associated
coordinately-methylated CpG island sequences. MethyLight MethyLight
CpG CpG Amplicon Amplicon Island Island GenBank Start End Start End
HUGO Gene Reaction Accession (GenBank (GenBank (GenBank (GenBank
Nomenclature Number Reaction ID Number Numbering) Numbering)
Numbering) Numbering) BCL2 HB-140 BCL2-M1 AY220759 1221 1304 746
1876 BDNF HB-258 BDNF-M2 AC103796 3794 3866 3351 4751 CACNA1G
HB-158 CACNA1G- AC021491 48345 48411 47327 49295 M1 CALCA HB-166
CALCA-M1 X15943 1706 1806 1614 2359 CRABP1 HB-197 CRABP1-M1
AC011270 122223 122142 122717 120620 DLEC1 HB-225 DLEC1-M1 AP006309
19959 20088 19425 20529 GATA3 HB-327 GATA3-M1 AL390294 51880 51959
50613 54089 HOXA1 HB-268 HOXA1-M2 AC004079 78220 78138 79793 77693
IGF2 HB-319 IGF2-M2 AC132217 108633 108720 106219 110017 KL HB-175
KL-M1 AB009667 2062 2189 1239 3185 NEUROG1 HB-261 NEUROG1- AC005738
75429 75342 76036 73946 M1 NR3C1 HB-067 NR3C1-M1 AY436590 1786 1860
32 3034 RUNX3 HB-181 RUNX3-M1 AL023096 64762 64646 67973 63661
SOCS1 HB-042 SOCS1-M1 AC009121 108803 108888 107037 109517
[0034] Despite the lack of an external gold standard for CIMP
classification, a comparison of cross-panel misclassification
errors and the associations with other molecular features strongly
indicates that the present novel panel outperforms a similar panel
of five traditional markers derived from the initial CIMP
classification paper (21) (MLH1, CDKN2A (p16.sup.INK4A), MINT1,
MINT2, and MINT31).
[0035] Applicants and others have reported a strong association
between CIMP+ status in colorectal adenocarcinomas and mutation of
the BRAF proto-oncogene (40, 42, 127, 131-133). With the present
new CIMP classification panel, there is a remarkably strong
association between CIMP+ status and BRAF mutation, with an odds
ration of 203 (95% confidence interval=41, 995), and a P-value of
1.6.times.10.sup.-21 (see below).
[0036] In a particular study presented herein, 24 out of 26 mutant
BRAF tumors were classified as CIMP+, while the 154 CIMP- tumors
contained only two mutant BRAF tumors, along with 152 BRAF wildtype
tumors (see also TABLE 4, Example 4).
TABLE-US-00002 TABLE 4 Distribution of covariates by New CIMP
Panel. CIMP+ CIMP- OVERALL (3-5 loci) (0-2 loci) VARIABLE N % N % N
% P-value TOTAL 187 100% 33 18% 154 82% SEX Male 103 55% 13 39% 90
58% Female 84 45% 20 61% 64 42% 0.05 SUBSITE Proximal 57 33% 19 59%
38 27% Distal 118 67% 13 41% 105 73% 0.0005 No Info 12 MSI MSI-high
21 11% 12 36% 9 6% STATUS MSI-low 19 10% 4 12% 15 10% MSS 147 79%
17 52% 130 84% 3.1.sup.-5 HNPCC Yes 8 4% 0 0% 8 5% STATUS No 179
96% 33 100% 146 95% 0.35 MLH1 Yes 16 9% 13 39% 3 2% METHYLATION No
171 91% 20 61% 151 98% 2.6.sup.-9 BRAF Mutant 26 14% 24 73% 2 1%
MUTATION WT 161 86% 9 27% 152 99% 1.6.sup.-21 KRAS Mutant 55 31% 3
10% 52 35% MUTATION WT 123 69% 28 90% 95 65% .002 No Info 9 MEAN
AGE (SD)* 65.5 68.6 64.8 0.13 (12.9) (12.7) (12.9) P-values are for
likelihood ratio tests from logistic regression with CIMP status as
the outcome. For the variable HNPCC we report the p-value from
Fisher's exact test. *Four CIMP- subjects are missing age.
[0037] Nevertheless, 9 out of 33 CIMP+ were wild-type for BRAF,
indicating that BRAF mutant tumors represent a large subset of
CIMP+ tumors, but that there are BRAF-independent CIMP+ tumors as
well. Therefore, BRAF mutation appears to arise almost only in the
context of CIMP+ status, indicating that this epigenetic phenomenon
dominates over this particular genetic alteration in human
colorectal cancer, which is a different conclusion from that
reached in the art (see, e.g., 38).
[0038] Additionally, all 8 of the hereditary non-polyposis
colorectal cancer (HNPCC)-associated MSI+ tumors were classified as
CIMP-, while 12 out of 13 non-HNPCC MSI+ tumors were classified as
CIMP+. On the other hand, 21 out of 33 of the CIMP+ tumors were
MSI-low or MSS (see below). Therefore, non-HNPCC MSI+ colorectal
tumors are a subset of CIMP+ tumors, but not the other way around,
indicating that sporadic MSI+ colorectal cancer is largely
attributable to the CIMP phenomenon. Thus, the present novel
markers enables a well-executed population-based studies of CIMP,
and a full etiologic understanding of MSI+ sporadic cancer.
[0039] Further aspects of the present invention, therefore, provide
a foundation for a population-based study of CIMP, by providing a
novel panel of very carefully selected methylation markers
representing the CIMP subgroup, and having utility to classify
CIMP.
DEFINITIONS
[0040] The term "Observed/Expected Ratio" ("O/E Ratio") refers to
the frequency of CpG dinucleotides within a particular DNA
sequence, and corresponds to the [number of CpG sites/(number of C
bases.times.number of G bases)]/band length for each fragment.
[0041] The term "CpG island" refers to a contiguous region of
genomic DNA that satisfies the criteria of (1) having a frequency
of CpG dinucleotides corresponding to an "Observed/Expected
Ratio">0.6, and (2) having a "GC Content">0.5. CpG islands
are typically, but not always, between about 0.2 to about 1 KB, or
to about 2 kb in length.
[0042] The term "methylation state" or "methylation status" refers
to the presence or absence of 5-methylcytosine ("5-mCyt") at one or
a plurality of CpG dinucleotides within a DNA sequence. Methylation
states at one or more particular CpG methylation sites (each having
two CpG dinucleotide sequences) within a DNA sequence include
"unmethylated," "fully-methylated" and "hemi-methylated."
[0043] The term "hemi-methylation" or "hemimethylation" refers to
the methylation state of a double stranded DNA wherein only one
strand thereof is methylated.
[0044] The term `AUC` as used herein is an abbreviation for the
area under a curve. In particular it refers to the area under a
Receiver Operating Characteristic (ROC) curve. The ROC curve is a
plot of the true positive rate against the false positive rate for
the different possible cut points of a diagnostic test. It shows
the trade-off between sensitivity and specificity depending on the
selected cut point (any increase in sensitivity will be accompanied
by a decrease in specificity). The area under an ROC curve (AUC) is
a measure for the accuracy of a diagnostic test (the larger the
area the better, optimum is 1, a random test would have a ROC curve
lying on the diagonal with an area of 0.5; for reference: J. P.
Egan. Signal Detection Theory and ROC Analysis, Academic Press, New
York, 1975).
[0045] The term "hypermethylation" refers to the average
methylation state corresponding to an increased presence of 5-mCyt
at one or a plurality of CpG dinucleotides within a DNA sequence of
a test DNA sample, relative to the amount of 5-mCyt found at
corresponding CpG dinucleotides within a normal control DNA
sample.
[0046] The term "hypomethylation" refers to the average methylation
state corresponding to a decreased presence of 5-mCyt at one or a
plurality of CpG dinucleotides within a DNA sequence of a test DNA
sample, relative to the amount of 5-mCyt found at corresponding CpG
dinucleotides within a normal control DNA sample.
[0047] The term "microarray" refers broadly to both "DNA
microarrays," and `DNA chip(s),` as recognized in the art,
encompasses all art-recognized solid supports, and encompasses all
methods for affixing nucleic acid molecules thereto or synthesis of
nucleic acids thereon.
[0048] "Genetic parameters" are mutations and polymorphisms of
genes and sequences further required for their regulation. To be
designated as mutations are, in particular, insertions, deletions,
point mutations, inversions and polymorphisms and, particularly
preferred, SNPs (single nucleotide polymorphisms).
[0049] "Epigenetic parameters" are, in particular, cytosine
methylation. Further epigenetic parameters include, for example,
the acetylation of histones which, however, cannot be directly
analysed using the described method but which, in turn, correlate
with the DNA methylation.
[0050] The term "bisulfite reagent" refers to a reagent comprising
bisulfite, disulfite, hydrogen sulfite or combinations thereof,
useful as disclosed herein to distinguish between methylated and
unmethylated CpG dinucleotide sequences.
[0051] The term "Methylation assay" refers to any assay for
determining the methylation state of one or more CpG dinucleotide
sequences within a sequence of DNA.
[0052] The term "MS.AP-PCR" (Methylation-Sensitive
Arbitrarily-Primed Polymerase Chain Reaction) refers to the
art-recognized technology that allows for a global scan of the
genome using CG-rich primers to focus on the regions most likely to
contain CpG dinucleotides, and described by Gonzalgo et al., Cancer
Research 57:594-599, 1997.
[0053] The term "MethyLight.TM." refers to the art-recognized
fluorescence-based real-time PCR technique described by Eads et
al., Cancer Res. 59:2302-2306, 1999.
[0054] The term "HeavyMethyl.TM." assay, in the embodiment thereof
implemented herein, refers to an assay, wherein methylation
specific blocking probes (also referred to herein as blockers)
covering CpG positions between, or covered by the amplification
primers enable methylation-specific selective amplification of a
nucleic acid sample.
[0055] The term "HeavyMethyl.TM. MethyLight.TM." assay, in the
embodiment thereof implemented herein, refers to a HeavyMethyl.TM.
MethyLight.TM. assay, which is a variation of the MethyLight.TM.
assay, wherein the MethyLight.TM. assay is combined with
methylation specific blocking probes covering CpG positions between
the amplification primers.
[0056] The term "Ms-SNuPE" (Methylation-sensitive Single Nucleotide
Primer Extension) refers to the art-recognized assay described by
Gonzalgo & Jones, Nucleic Acids Res. 25:2529-2531, 1997.
[0057] The term "MSP" (Methylation-specific PCR) refers to the
art-recognized methylation assay described by Herman et al. Proc.
Natl. Acad. Sci. USA 93:9821-9826, 1996, and by U.S. Pat. No.
5,786,146.
[0058] The term "COBRA" (Combined Bisulfite Restriction Analysis)
refers to the art-recognized methylation assay described by Xiong
& Laird, Nucleic Acids Res. 25:2532-2534, 1997.
[0059] The term "MCA" (Methylated CpG Island Amplification) refers
to the methylation assay described by Toyota et al., Cancer Res.
59:2307-12, 1999, and in WO 00/26401A1.
[0060] The term "hybridisation" is to be understood as a bond of an
oligonucleotide to a complementary sequence along the lines of the
Watson-Crick base pairings in the sample DNA, forming a duplex
structure.
[0061] "Stringent hybridisation conditions," as defined herein,
involve hybridising at 68.degree. C. in
5.times.SSC/5.times.Denhardt's solution/1.0% SDS, and washing in
0.2.times.SSC/0.1% SDS at room temperature, or involve the
art-recognized equivalent thereof (e.g., conditions in which a
hybridisation is carried out at 60.degree. C. in 2.5.times.SSC
buffer, followed by several washing steps at 37.degree. C. in a low
buffer concentration, and remains stable). Moderately stringent
conditions, as defined herein, involve including washing in
3.times.SSC at 42.degree. C., or the art-recognized equivalent
thereof. The parameters of salt concentration and temperature can
be varied to achieve the optimal level of identity between the
probe and the target nucleic acid. Guidance regarding such
conditions is available in the art, for example, by Sambrook et
al., 1989, Molecular Cloning, A Laboratory Manual, Cold Spring
Harbor Press, N.Y.; and Ausubel et al. (eds.), 1995, Current
Protocols in Molecular Biology, (John Wiley & Sons, N.Y.) at
Unit 2.10.
[0062] The terms "Methylation-specific restriction enzymes" or
"methylation-sensitive restriction enzymes" shall be taken to mean
an enzyme that selectively digests a nucleic acid dependant on the
methylation state of its recognition site. In the case of such
restriction enzymes which specifically cut if the recognition site
is not methylated or hemimethylated, the cut will not take place,
or with a significantly reduced efficiency, if the recognition site
is methylated. In the case of such restriction enzymes which
specifically cut if the recognition site is methylated, the cut
will not take place, or with a significantly reduced efficiency if
the recognition site is not methylated. Preferred are
methylation-specific restriction enzymes, the recognition sequence
of which contains a CG dinucleotide (for instance cgcg or cccggg).
Further preferred for some embodiments are restriction enzymes that
do not cut if the cytosine in this dinucleotide is methylated at
the carbon atom C5.
[0063] "Non-methylation-specific restriction enzymes" or
"non-methylation-sensitive restriction enzymes" are restriction
enzymes that cut a nucleic acid sequence irrespective of the
methylation state with nearly identical efficiency. They are also
called "methylation-unspecific restriction enzymes."
[0064] The term "gene" shall be taken to include all transcript
variants thereof (e.g. the term "NEUROG1" shall include for example
its transcripts and any truncated transcript, etc) and all promoter
and regulatory elements thereof. Furthermore as a plurality of SNPs
are known within said gene the term shall be taken to include all
sequence variants thereof.
[0065] The term "pre-cancerous" or "pre-neoplastic" and equivalents
thereof shall be taken to mean any cellular proliferative disorder
which is undergoing malignant transformation. Examples of such
conditions include, in the context of colorectal cellular
proliferative disorders, cellular proliferative disorders with a
high degree of dysplasia and the following classes of adenomas:
[0066] Level 1: penetration of malignant glands through the
muscularis mucosa into the submucosa, within the polyp head;
[0067] Level 2: the same submucosal invasion, but present at the
junction of the head to the stalk;
[0068] Level 3: invasion of the stalk; and
[0069] Level 4: invasion of the stalk's base at the connection to
the colonic wall (this level corresponds to stage Dukes A).
Exemplary Preferred Embodiments
[0070] In particular aspects, the present invention provides
compositions and methods for at least one of: determining and/or
classifying CIMP status in colorectal cancers; identifying KRAS
mutant, BRAF wildtype tumors; determining the relationship between
CIMP status and other molecular features of the cancers including,
but not limited to BRAF mutation, KRAS mutation and MSI status;
determining the relationship between CIMP status and other
variables including, but not limited to age, sex, tumor location,
family history, race, country of origin, tumor characteristics
(including, tumor type, tumor grade, invasive margin
characteristics, lymphocyte infiltration characteristics, direct
spread, lymph node spread, venous spread and type of residual
adjacent polyp, if present); determining, between subgroups defined
by CIMP status and BRAF mutations, effects of selected risk factors
including, but not limited to body mass index, smoking history,
alcohol intake, dietary folate intake, folate metabolic enzyme
polymorphisms and history of hormonal use; and providing a
foundation for a population-based study of CIMP, by providing a
novel panel of carefully selected methylation markers representing
the CIMP subgroup, and having utility to classify CIMP. Said
methods comprising determining the methylation status or the
expression levels of at least one gene or genomic sequence selected
from the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1,
DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table
6); SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively) in a biological sample
isolated from said subject wherein underexpression and/or CpG
methylation is indicative of the presence or class of said
disorder. Said markers may be used for the methods listed above,
and for diagnosis of neoplastic cellular proliferative disorders
(cancer), including early detection during the pre-cancerous stages
of the disease, and furthermore for the differentiation of
neoplastic from benign cellular proliferative disorders. In
particular aspects, the present invention discloses a method
wherein a neoplastic cell proliferative disorder is distinguished
from a benign cell proliferative disorder said method characterized
in that underexpression and/or the presence of CpG methylation is
indicative of the presence of a neoplastic cell proliferative
disorder or pre-neoplastic disorder and the absence thereof is
indicative of the presence of a benign cell proliferative
disorder.
[0071] The markers of the present invention are particularly
efficient in detecting or distinguishing between colorectal cell
proliferative disorders, thereby providing improved means for the
early detection, classification and treatment of said
disorders.
[0072] In addition to the embodiments above wherein the methylation
analysis of at least one gene or genomic sequence selected from the
group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) is analysed, the invention presents
further panels of genes comprising at least one gene or genomic
sequence selected from the group consisting of BCL2, BDNF, CACNA1G,
CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1,
RUNX3, SOCS1 (Table 6); SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively) with
novel utility for the detection of cancers, in particular
colorectal cancer.
[0073] In a first further embodiment the present invention is based
upon the analysis of CpG methylaton status of at least one gene or
genomic sequence selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); SEQ ID NOS:128-141, 114-127 and
100-113 (respective genomic, CpG island and amplicons,
respectively).
[0074] Bisulfite Modification of DNA is an Art-Recognized Tool Used
to Assess CpG Methylation Status.
[0075] 5-methylcytosine is the most frequent covalent base
modification in the DNA of eukaryotic cells. It plays a role, for
example, in the regulation of the transcription, in genetic
imprinting, and in tumorigenesis. Therefore, the identification of
5-methylcytosine as a component of genetic information is of
considerable interest. However, 5-methylcytosine positions cannot
be identified by sequencing, because 5-methylcytosine has the same
base pairing behavior as cytosine. Moreover, the epigenetic
information carried by 5-methylcytosine is completely lost during,
e.g., PCR amplification.
[0076] The most frequently used method for analyzing DNA for the
presence of 5-methylcytosine is based upon the specific reaction of
bisulfite with cytosine whereby, upon subsequent alkaline
hydrolysis, cytosine is converted to uracil which corresponds to
thymine in its base pairing behavior. Significantly, however,
5-methylcytosine remains unmodified under these conditions.
Consequently, the original DNA is converted in such a manner that
methylcytosine, which originally could not be distinguished from
cytosine by its hybridization behavior, can now be detected as the
only remaining cytosine using standard, art-recognized molecular
biological techniques, for example, by amplification and
hybridization, or by sequencing. All of these techniques are based
on differential base pairing properties, which can now be fully
exploited.
[0077] The prior art, in terms of sensitivity, is defined by a
method comprising enclosing the DNA to be analysed in an agarose
matrix, thereby preventing the diffusion and renaturation of the
DNA (bisulfite only reacts with single-stranded DNA), and replacing
all precipitation and purification steps with fast dialysis (Olek
A, et al., A modified and improved method for bisulfite based
cytosine methylation analysis, Nucleic Acids Res. 24:5064-6, 1996).
It is thus possible to analyse individual cells for methylation
status, illustrating the utility and sensitivity of the method. An
overview of art-recognized methods for detecting 5-methylcytosine
is provided by Rein, T., et al., Nucleic Acids Res., 26:2255,
1998.
[0078] The bisulfite technique, barring few exceptions (e.g.,
Zeschnigk M, et al., Eur J Hum Genet. 5:94-98, 1997), is currently
only used in research. In all instances, short, specific fragments
of a known gene are amplified subsequent to a bisulfite treatment,
and either completely sequenced (Olek & Walter, Nat. Genet.
1997 17:275-6, 1997), subjected to one or more primer extension
reactions (Gonzalgo & Jones, Nucleic Acids Res., 25:2529-31,
1997; WO 95/00669; U.S. Pat. No. 6,251,594) to analyse individual
cytosine positions, or treated by enzymatic digestion (Xiong &
Laird, Nucleic Acids Res., 25:2532-4, 1997). Detection by
hybridisation has also been described in the art (Olek et al., WO
99/28498). Additionally, use of the bisulfite technique for
methylation detection with respect to individual genes has been
described (Grigg & Clark, Bioessays, 16:431-6, 1994; Zeschnigk
M, et al., Hum Mol Genet., 6:387-95, 1997; Feil R, et al., Nucleic
Acids Res., 22:695-, 1994; Martin V, et al., Gene, 157:261-4, 1995;
WO 9746705 and WO 9515373).
[0079] The present invention provides for the use of the bisulfite
technique, in combination with one or more methylation assays, for
determination of the methylation status of CpG dinucleotide
sequences within at least one sequence selected from the group
consisting of SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively). Genomic CpG
dinucleotides can be methylated or unmethylated (alternatively
known as up- and down-methylated respectively). However the methods
of the present invention are suitable for the analysis of
biological samples of a heterogeneous nature, e.g., a low
concentration of tumor cells within a background of blood or stool.
Accordingly, when analyzing the methylation status of a CpG
position within such a sample the person skilled in the art may use
a quantitative assay for determining the level (e.g., percent,
fraction, ratio, proportion or degree) of methylation at a
particular CpG position as opposed to a methylation state.
Accordingly the term methylation status or methylation state should
also be taken to mean a value reflecting the degree of methylation
at a CpG position. Unless specifically stated the terms
"hypermethylated" or "upmethylated" shall be taken to mean a
methylation level above that of a specified cut-off point, wherein
said cut-off may be a value representing the average or median
methylation level for a given population, or is preferably an
optimized cut-off level. The "cut-off" is also referred herein as a
"threshold". In the context of the present invention the terms
"methylated", "hypermethylated" or "upmethylated" shall be taken to
include a methylation level above the cut-off be zero (0) % (or
equivalents thereof) methylation for all CpG positions within and
associated with (e.g. in promoter or regulatory regions) the genes
or genomic sequence selected from the group consisting of BCL2,
BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); SEQ ID NOS:128-141, 114-127
and 100-113 (respective genomic, CpG island and amplicons,
respectively).
[0080] According to the present invention, determination of the
methylation status of CpG dinucleotide sequences BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) has utility both in the diagnosis and
characterization of CIMP.
[0081] Methylation Assay Procedures.
[0082] Various methylation assay procedures are known in the art,
and can be used in conjunction with the present invention. These
assays allow for determination of the methylation state of one or a
plurality of CpG dinucleotides (e.g., CpG islands) within a DNA
sequence. Such assays involve, among other techniques, DNA
sequencing of bisulfite-treated DNA, PCR (for sequence-specific
amplification), Southern blot analysis, and use of
methylation-sensitive restriction enzymes.
[0083] For example, genomic sequencing has been simplified for
analysis of DNA methylation patterns and 5-methylcytosine
distribution by using bisulfite treatment (Frommer et al., Proc.
Natl. Acad. Sci. USA 89:1827-1831, 1992). Additionally, restriction
enzyme digestion of PCR products amplified from bisulfite-converted
DNA is used, e.g., the method described by Sadri & Hornsby
(Nucl. Acids Res. 24:5058-5059, 1996), or COBRA (Combined Bisulfite
Restriction Analysis) (Xiong & Laird, Nucleic Acids Res.
25:2532-2534, 1997).
[0084] COBRA.
[0085] COBRA.TM. analysis is a quantitative methylation assay
useful for determining DNA methylation levels at specific gene loci
in small amounts of genomic DNA (Xiong & Laird, Nucleic Acids
Res. 25:2532-2534, 1997). Briefly, restriction enzyme digestion is
used to reveal methylation-dependent sequence differences in PCR
products of sodium bisulfite-treated DNA. Methylation-dependent
sequence differences are first introduced into the genomic DNA by
standard bisulfite treatment according to the procedure described
by Frommer et al. (Proc. Natl. Acad. Sci. USA 89:1827-1831, 1992).
PCR amplification of the bisulfite converted DNA is then performed
using primers specific for the CpG islands of interest, followed by
restriction endonuclease digestion, gel electrophoresis, and
detection using specific, labeled hybridization probes. Methylation
levels in the original DNA sample are represented by the relative
amounts of digested and undigested PCR product in a linearly
quantitative fashion across a wide spectrum of DNA methylation
levels. In addition, this technique can be reliably applied to DNA
obtained from microdissected paraffin-embedded tissue samples.
[0086] Typical reagents (e.g., as might be found in a typical
COBRA.TM.-based kit) for COBRA.TM. analysis may include, but are
not limited to: PCR primers for specific gene (or bisulfite treated
DNA sequence or CpG island); restriction enzyme and appropriate
buffer; gene-hybridization oligonucleotide; control hybridization
oligonucleotide; kinase labeling kit for oligonucleotide probe; and
labeled nucleotides. Additionally, bisulfite conversion reagents
may include: DNA denaturation buffer; sulfonation buffer; DNA
recovery reagents or kits (e.g., precipitation, ultrafiltration,
affinity column); desulfonation buffer; and DNA recovery
components.
[0087] Preferably, assays such as "MethyLight.TM." (a
fluorescence-based real-time PCR technique) (Eads et al., Cancer
Res. 59:2302-2306, 1999), Ms-SNuPE.TM. (Methylation-sensitive
Single Nucleotide Primer Extension) reactions (Gonzalgo &
Jones, Nucleic Acids Res. 25:2529-2531, 1997), methylation-specific
PCR ("MSP"; Herman et al., Proc. Natl. Acad. Sci. USA 93:9821-9826,
1996; U.S. Pat. No. 5,786,146), and methylated CpG island
amplification ("MCA"; Toyota et al., Cancer Res. 59:2307-12, 1999)
are used alone or in combination with other of these methods.
[0088] The "HeavyMethyl.TM." assay, technique is a quantitative
method for assessing methylation differences based on methylation
specific amplification of bisulfite treated DNA. Methylation
specific blocking probes (also referred to herein as blockers)
covering CpG positions between, or covered by the amplification
primers enable methylation-specific selective amplification of a
nucleic acid sample.
[0089] The term "HeavyMethyl.TM. MethyLight.TM." assay, in the
embodiment thereof implemented herein, refers to a HeavyMethyl.TM.
MethyLight.TM. assay, which is a variation of the MethyLight.TM.
assay, wherein the MethyLight.TM. assay is combined with
methylation specific blocking probes covering CpG positions between
the amplification primers. The HeavyMethyl.TM. assay may also be
used in combination with methylation specific amplification
primers.
[0090] Typical reagents (e.g., as might be found in a typical
MethyLight.TM.-based kit) for HeavyMethyl.TM. analysis may include,
but are not limited to: PCR primers for specific genes (or
bisulfite treated DNA sequence or CpG island); blocking
oligonucleotides; optimized PCR buffers and deoxynucleotides; and
Taq polymerase.
[0091] MSP.
[0092] MSP (methylation-specific PCR) allows for assessing the
methylation status of virtually any group of CpG sites within a CpG
island, independent of the use of methylation-sensitive restriction
enzymes (Herman et al. Proc. Natl. Acad. Sci. USA 93:9821-9826,
1996; U.S. Pat. No. 5,786,146). Briefly, DNA is modified by sodium
bisulfite converting all unmethylated, but not methylated cytosines
to uracil, and subsequently amplified with primers specific for
methylated versus unmethylated DNA. MSP requires only small
quantities of DNA, is sensitive to 0.1% methylated alleles of a
given CpG island locus, and can be performed on DNA extracted from
paraffin-embedded samples. Typical reagents (e.g., as might be
found in a typical MSP-based kit) for MSP analysis may include, but
are not limited to: methylated and unmethylated PCR primers for
specific gene (or bisulfite treated DNA sequence or CpG island),
optimized PCR buffers and deoxynucleotides, and specific
probes.
[0093] MethyLight.TM..
[0094] The MethyLight.TM. assay is a high-throughput quantitative
methylation assay that utilizes fluorescence-based real-time PCR
(TaqMan.RTM.) technology that requires no further manipulations
after the PCR step (Eads et al., Cancer Res. 59:2302-2306, 1999).
Briefly, the MethyLight.TM. process begins with a mixed sample of
genomic DNA that is converted, in a sodium bisulfite reaction, to a
mixed pool of methylation-dependent sequence differences according
to standard procedures (the bisulfite process converts unmethylated
cytosine residues to uracil). Fluorescence-based PCR is then
performed in a "biased" (with PCR primers that overlap known CpG
dinucleotides) reaction. Sequence discrimination can occur both at
the level of the amplification process and at the level of the
fluorescence detection process.
[0095] The MethyLight.TM. assay may be used as a quantitative test
for methylation patterns in the genomic DNA sample, wherein
sequence discrimination occurs at the level of probe hybridization.
In this quantitative version, the PCR reaction provides for a
methylation specific amplification in the presence of a fluorescent
probe that overlaps a particular putative methylation site. An
unbiased control for the amount of input DNA is provided by a
reaction in which neither the primers, nor the probe overlie any
CpG dinucleotides. Alternatively, a qualitative test for genomic
methylation is achieved by probing of the biased PCR pool with
either control oligonucleotides that do not "cover" known
methylation sites (a fluorescence-based version of the
HeavyMethyl.TM. and MSP techniques), or with oligonucleotides
covering potential methylation sites.
[0096] The MethyLight.TM. process can by used with any suitable
probes e.g. "TaqMan.RTM.", Lightcycler.RTM. etc. . . . . For
example, double-stranded genomic DNA is treated with sodium
bisulfite and subjected to one of two sets of PCR reactions using
TaqMan.RTM. probes; e.g., with MSP primers and/or HeavyMethyl
blocker oligonucleotides and TaqMan.RTM. probe. The TaqMan.RTM.
probe is dual-labeled with fluorescent "reporter" and "quencher"
molecules, and is designed to be specific for a relatively high GC
content region so that it melts out at about 10.degree. C. higher
temperature in the PCR cycle than the forward or reverse primers.
This allows the TaqMan.RTM. probe to remain fully hybridized during
the PCR annealing/extension step. As the Taq polymerase
enzymatically synthesizes a new strand during PCR, it will
eventually reach the annealed TaqMan.RTM. probe. The Taq polymerase
5' to 3' endonuclease activity will then displace the TaqMan.RTM.
probe by digesting it to release the fluorescent reporter molecule
for quantitative detection of its now unquenched signal using a
real-time fluorescent detection system.
[0097] Typical reagents (e.g., as might be found in a typical
MethyLight.TM.-based kit) for MethyLight.TM. analysis may include,
but are not limited to: PCR primers for specific gene (or bisulfite
treated DNA sequence or CpG island); TaqMan.RTM. or
Lightcycler.RTM. probes; optimized PCR buffers and
deoxynucleotides; and Taq polymerase.
[0098] The QM.TM. (quantitative methylation) assay is an
alternative quantitative test for methylation patterns in genomic
DNA samples, wherein sequence discrimination occurs at the level of
probe hybridization. In this quantitative version, the PCR reaction
provides for unbiased amplification in the presence of a
fluorescent probe that overlaps a particular putative methylation
site. An unbiased control for the amount of input DNA is provided
by a reaction in which neither the primers, nor the probe overlie
any CpG dinucleotides. Alternatively, a qualitative test for
genomic methylation is achieved by probing of the biased PCR pool
with either control oligonucleotides that do not "cover" known
methylation sites (a fluorescence-based version of the
HeavyMethyl.TM. and MSP techniques), or with oligonucleotides
covering potential methylation sites.
[0099] The QM.TM. process can by used with any suitable probes e.g.
"TaqMan.RTM.", Lightcycler.RTM. etc. . . . in the amplification
process. For example, double-stranded genomic DNA is treated with
sodium bisulfite and subjected to unbiased primers and the
TaqMan.RTM. probe. The TaqMan.RTM. probe is dual-labeled with
fluorescent "reporter" and "quencher" molecules, and is designed to
be specific for a relatively high GC content region so that it
melts out at about 10.degree. C. higher temperature in the PCR
cycle than the forward or reverse primers. This allows the
TaqMan.RTM. probe to remain fully hybridized during the PCR
annealing/extension step. As the Taq polymerase enzymatically
synthesizes a new strand during PCR, it will eventually reach the
annealed TaqMan.RTM. probe. The Taq polymerase 5' to 3'
endonuclease activity will then displace the TaqMan.RTM. probe by
digesting it to release the fluorescent reporter molecule for
quantitative detection of its now unquenched signal using a
real-time fluorescent detection system. Typical reagents (e.g., as
might be found in a typical QM.TM.-based kit) for QM.TM. analysis
may include, but are not limited to: PCR primers for specific gene
(or bisulfite treated DNA sequence or CpG island); TaqMan.RTM. or
Lightcycler.RTM. probes; optimized PCR buffers and
deoxynucleotides; and Taq polymerase.
[0100] Ms-SNuPE
[0101] The Ms-SNuPE.TM. technique is a quantitative method for
assessing methylation differences at specific CpG sites based on
bisulfite treatment of DNA, followed by single-nucleotide primer
extension (Gonzalgo & Jones, Nucleic Acids Res. 25:2529-2531,
1997). Briefly, genomic DNA is reacted with sodium bisulfite to
convert unmethylated cytosine to uracil while leaving
5-methylcytosine unchanged. Amplification of the desired target
sequence is then performed using PCR primers specific for
bisulfite-converted DNA, and the resulting product is isolated and
used as a template for methylation analysis at the CpG site(s) of
interest. Small amounts of DNA can be analyzed (e.g.,
microdissected pathology sections), and it avoids utilization of
restriction enzymes for determining the methylation status at CpG
sites.
[0102] Typical reagents (e.g., as might be found in a typical
Ms-SNuPE.TM.-based kit) for Ms-SNuPE.TM. analysis may include, but
are not limited to: PCR primers for specific gene (or bisulfite
treated DNA sequence or CpG island); optimized PCR buffers and
deoxynucleotides; gel extraction kit; positive control primers;
Ms-SNuPE.TM. primers for specific gene; reaction buffer (for the
Ms-SNuPE reaction); and labelled nucleotides. Additionally,
bisulfite conversion reagents may include: DNA denaturation buffer;
sulfonation buffer; DNA recovery regents or kit (e.g.,
precipitation, ultrafiltration, affinity column); desulfonation
buffer; and DNA recovery components.
The Genomic Sequence According to SEQ ID NOS:128-141, 114-127 and
100-113 (Respective Genomic, CpG Island and Amplicons,
Respectively), and Non-Naturally Occurring Treated Variants Thereof
According to SEQ ID NOS:170-197, 226-253, 142-169 and 198-225, were
Determined to have Novel Utility for the Detection, Classification
and/or Treatment of CIMP, in Particular Colorectal Cell
Proliferative Disorders
[0103] In one embodiment the invention of the method comprises the
following steps: i) contacting genomic DNA (preferably isolated
from body fluids) obtained from the subject with at least one
reagent, or series of reagents that distinguishes between
methylated and non-methylated CpG dinucleotides within at least one
gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) (including their promoter and
regulatory regions); and ii) detecting, or detecting and
distinguishing CIMP or colon proliferative disorders (e.g.,
afforded with a sensitivity of greater than or equal to 80% and a
specificity of greater than or equal to 80%).
[0104] Preferably, the sensitivity is from about 75% to about 96%,
or from about 80% to about 90%, or from about 80% to about 85%.
Preferably, the specificity is from about 75% to about 96%, or from
about 80% to about 90%, or from about 80% to about 85%.
[0105] Genomic DNA may be isolated by any means standard in the
art, including the use of commercially available kits. Briefly,
wherein the DNA of interest is encapsulated in by a cellular
membrane the biological sample must be disrupted and lysed by
enzymatic, chemical or mechanical means. The DNA solution may then
be cleared of proteins and other contaminants, e.g., by digestion
with proteinase K. The genomic DNA is then recovered from the
solution. This may be carried out by means of a variety of methods
including salting out, organic extraction or binding of the DNA to
a solid phase support. The choice of method will be affected by
several factors including time, expense and required quantity of
DNA. All clinical sample types comprising neoplastic matter or
pre-neoplastic matter are suitable for use in the present method,
preferred are cell lines, histological slides, biopsies,
paraffin-embedded tissue, body fluids, stool, colonic effluent,
urine, blood plasma, blood serum, whole blood, isolated blood
cells, cells isolated from the blood and combinations thereof. Body
fluids are the preferred source of the DNA; particularly preferred
are blood plasma, blood serum, whole blood, isolated blood cells
and cells isolated from the blood.
[0106] The genomic DNA sample is then treated with at least one
reagent, or series of reagents that distinguishes between
methylated and non-methylated CpG dinucleotides within at least one
target region of the genomic DNA, wherein the target region
comprises, or hybridizes under stringent conditions to a sequence
of at least 16 contiguous nucleotides of at least one sequence
selected from the group consisting of SEQ ID NOS:128-141, 114-127
and 100-113 (respective genomic, CpG island and amplicons,
respectively), wherein said contiguous nucleotides comprise at
least one CpG dinucleotide sequence.
[0107] It is particularly preferred that said reagent converts
cytosine bases which are unmethylated at the 5'-position to uracil,
thymine, or another base which is dissimilar to cytosine in terms
of hybridisation behaviour. However in an alternative embodiment
said reagent may be a methylation sensitive restriction enzyme.
[0108] Wherein the genomic DNA sample is treated in such a manner
that cytosine bases which are unmethylated at the 5'-position are
converted to uracil, thymine, or another base which is dissimilar
to cytosine in terms of hybridization behavior. It is preferred
that this treatment is carried out with bisulfate (hydrogen
sulfite, disulfite) and subsequent alkaline hydrolysis. Such a
treatment results in the conversion of SEQ ID NOS:128-141, 114-127
and 100-113 (respective genomic, CpG island and amplicons,
respectively) to SEQ ID NOS:170-197 and 142-169, (respectively)
wherein said CpG dinucleotides are methylated or SEQ ID NOS:226-253
and 198-225 wherein said CpG dinucleotides are unmethylated.
[0109] The treated DNA is then analysed in order to determine the
methylation state of the target gene sequences (at least one gene
or genomic sequence selected from the group consisting of BCL2,
BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) prior to the treatment). It is
particularly preferred that the target region comprises, or
hybridizes under stringent conditions to at least 16 contiguous
nucleotides of at least one gene or genomic sequence selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively). It is preferred
that the sequence of said genes according to SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) are analysed. The method of analysis may be selected
from those known in the art, including those listed herein.
Particularly preferred are MethyLight.TM., MSP and the use of
blocking oligonucleotides (HeavyMethyl.TM.) as described herein. It
is further preferred that any oligonucleotides used in such
analysis (including primers, blocking oligonucleotides and
detection probes) should be reverse complementary, identical, or
hybridise under stringent or highly stringent conditions to an at
least 16-base-pair long segment of the base sequences of one or
more of SEQ ID NOS:170-197, 226-253, 142-169 and 198-225 and
sequences complementary thereto.
[0110] Aberrant methylation, more specifically hypermethylation of
the genes or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) (including their promoter and/or
regulatory regions) is associated with the presence of CIMP, and is
particularly prevalent in colorectal carcinomas. Accordingly, in
certain embodiments, wherein a biological sample presents within
methylation as disclosed herein, said sample should be determined
as CIMP.
[0111] Analysis of one the genes or genomic sequence selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively) enables for the
first time detecting, or detecting and distinguishing CIMP or colon
cell proliferative disorders (e.g., afforded with a sensitivity of
greater than or equal to 80% and a specificity of greater than or
equal to 80%. Sensitivity is calculated as: (detected neoplasia/all
neoplasia; e.g., (detected colon neoplasia/all colon neoplasia);
and specificity is calculated as (non-detected negatives/total
negatives)).
[0112] Preferably, the sensitivity is from about 75% to about 96%,
or from about 80% to about 90%, or from about 80% to about 85%.
Preferably, the specificity is from about 75% to about 96%, or from
about 80% to about 90%, or from about 80% to about 85%.
[0113] For certain embodiments, colon neoplasia is herein defined
as all colon malignancies and adenomas greater than 1 cm., or
subsets thereof. Negatives can be defined as healthy
individuals.
[0114] In one embodiment the method discloses the use of at least
one gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) (or promoter and/or regulatory regions
thereof) as a marker for detection and distinguishing of CIMP, etc.
as described herein.
[0115] Said method may be enabled by means of any analysis of the
expression of an RNA transcribed therefrom or polypeptide or
protein translated from said RNA, preferably by means of mRNA
expression analysis or polypeptide expression analysis. Accordingly
the present invention also provides diagnostic assays and methods,
both quantitative and qualitative for detecting the expression of
at least one gene or genomic sequence selected from the group
consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3,
HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g.,
within SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively) in a subject and
determining therefrom upon the presence or absence of, e.g., CIMP,
etc., in said subject.
[0116] Aberrant expression of mRNA transcribed from the genes or
genomic sequences selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) are associated with the presence of CIMP, cancer in a
subject. According to particular aspects of the present invention,
under expression (and/or presence methylation) is associated with
the presence of CIMP, colon cancer, and vice versa over-expression
(and/or absence of methylation) is associated with the absence of
CIMP, colon cancer.
[0117] To detect the presence of mRNA encoding a gene or genomic
sequence, a sample is obtained from a patient. The sample may be
any suitable sample comprising cellular matter of the tumour.
Suitable sample types include cell lines, histological slides,
biopsies, paraffin-embedded tissue, body fluids, stool, colonic
effluent, urine, blood plasma, blood serum, whole blood, isolated
blood cells, cells isolated from the blood and all possible
combinations thereof. It is preferred that said sample types are
stool or body fluids selected from the group consisting colonic
effluent, urine, blood plasma, blood serum, whole blood, isolated
blood cells, cells isolated from the blood.
[0118] The sample may be treated to extract the RNA contained
therein. The resulting nucleic acid from the sample is then
analyzed. Many techniques are known in the state of the art for
determining absolute and relative levels of gene expression,
commonly used techniques suitable for use in the present invention
include in situ hybridisation (e.g., FISH), Northern analysis,
RNase protection assays (RPA), microarrays and PCR-based
techniques, such as quantitative PCR and differential display PCR
or any other nucleic acid detection method.
[0119] Particularly preferred is the use of the reverse
transcription/polymerisation chain reaction technique (RT-PCR). The
method of RT-PCR is well known in the art (for example, see Watson
and Fleming, supra).
[0120] The RT-PCR method can be performed as follows. Total
cellular RNA is isolated by, for example, the standard guanidium
isothiocyanate method and the total RNA is reverse transcribed. The
reverse transcription method involves synthesis of DNA on a
template of RNA using a reverse transcriptase enzyme and a 3' end
oligonucleotide dT primer and/or random hexamer primers. The cDNA
thus produced is then amplified by means of PCR. (Belyaysky et al,
Nucl Acid Res 17:2919-2932, 1989; Krug and Berger, Methods in
Enzymology, Academic Press, N.Y., Vol. 152, pp. 316-325, 1987 which
are incorporated by reference). Further preferred is the
"Real-time" variant of RT-PCR, wherein the PCR product is detected
by means of hybridisation probes (e.g. TaqMan, Lightcycler,
Molecular Beacons & Scorpion) or SYBR green. The detected
signal from the probes or SYBR green is then quantitated either by
reference to a standard curve or by comparing the Ct values to that
of a calibration standard. Analysis of housekeeping genes is often
used to normalize the results.
[0121] In Northern blot analysis total or poly(A)+ mRNA is run on a
denaturing agarose gel and detected by hybridisation to a labelled
probe in the dried gel itself or on a membrane. The resulting
signal is proportional to the amount of target RNA in the RNA
population.
[0122] Comparing the signals from two or more cell populations or
tissues reveals relative differences in gene expression levels.
Absolute quantitation can be performed by comparing the signal to a
standard curve generated using known amounts of an in vitro
transcript corresponding to the target RNA. Analysis of
housekeeping genes, genes whose expression levels are expected to
remain relatively constant regardless of conditions, is often used
to normalize the results, eliminating any apparent differences
caused by unequal transfer of RNA to the membrane or unequal
loading of RNA on the gel.
[0123] The first step in Northern analysis is isolating pure,
intact RNA from the cells or tissue of interest. Because Northern
blots distinguish RNAs by size, sample integrity influences the
degree to which a signal is localized in a single band. Partially
degraded RNA samples will result in the signal being smeared or
distributed over several bands with an overall loss in sensitivity
and possibly an erroneous interpretation of the data. In Northern
blot analysis, DNA, RNA and oligonucleotide probes can be used and
these probes are preferably labelled (e.g., radioactive labels,
mass labels or fluorescent labels). The size of the target RNA, not
the probe, will determine the size of the detected band, so methods
such as random-primed labelling, which generates probes of variable
lengths, are suitable for probe synthesis. The specific activity of
the probe will determine the level of sensitivity, so it is
preferred that probes with high specific activities, are used.
[0124] In an RNase protection assay, the RNA target and an RNA
probe of a defined length are hybridised in solution. Following
hybridisation, the RNA is digested with RNases specific for
single-stranded nucleic acids to remove any unhybridized,
single-stranded target RNA and probe. The RNases are inactivated,
and the RNA is separated e.g. by denaturing polyacrylamide gel
electrophoresis. The amount of intact RNA probe is proportional to
the amount of target RNA in the RNA population. RPA can be used for
relative and absolute quantitation of gene expression and also for
mapping RNA structure, such as intron/exon boundaries and
transcription start sites. The RNase protection assay is preferable
to Northern blot analysis as it generally has a lower limit of
detection.
[0125] The antisense RNA probes used in RPA are generated by in
vitro transcription of a DNA template with a defined endpoint and
are typically in the range of 50-600 nucleotides. The use of RNA
probes that include additional sequences not homologous to the
target RNA allows the protected fragment to be distinguished from
the full-length probe. RNA probes are typically used instead of DNA
probes due to the ease of generating single-stranded RNA probes and
the reproducibility and reliability of RNA:RNA duplex digestion
with RNases (Ausubel et al. 2003), particularly preferred are
probes with high specific activities.
[0126] Particularly preferred is the use of microarrays. The
microarray analysis process can be divided into two main parts.
First is the immobilization of known gene sequences onto glass
slides or other solid support followed by hybridisation of the
fluorescently labelled cDNA (comprising the sequences to be
interrogated) to the known genes immobilized on the glass slide (or
other solid phase). After hybridisation, arrays are scanned using a
fluorescent microarray scanner. Analysing the relative fluorescent
intensity of different genes provides a measure of the differences
in gene expression.
[0127] DNA arrays can be generated by immobilizing presynthesized
oligonucleotides onto prepared glass slides or other solid
surfaces. In this case, representative gene sequences are
manufactured and prepared using standard oligonucleotide synthesis
and purification methods. These synthesized gene sequences are
complementary to the RNA transcript(s) of the genes of interest (in
this case the genes or genomic sequences selected from the group
consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3,
HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g.,
within SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively) and tend to be shorter
sequences in the range of 25-70 nucleotides. Alternatively,
immobilized oligos can be chemically synthesized in situ on the
surface of the slide. In situ oligonucleotide synthesis involves
the consecutive addition of the appropriate nucleotides to the
spots on the microarray; spots not receiving a nucleotide are
protected during each stage of the process using physical or
virtual masks. Preferably said synthesized nucleic acids are locked
nucleic acids.
[0128] In expression profiling microarray experiments, the RNA
templates used are representative of the transcription profile of
the cells or tissues under study. RNA is first isolated from the
cell populations or tissues to be compared. Each RNA sample is then
used as a template to generate fluorescently labelled cDNA via a
reverse transcription reaction. Fluorescent labelling of the cDNA
can be accomplished by either direct labelling or indirect
labelling methods. During direct labelling, fluorescently modified
nucleotides (e.g., Cy.RTM.3- or Cy.RTM.5-dCTP) are incorporated
directly into the cDNA during the reverse transcription.
Alternatively, indirect labelling can be achieved by incorporating
aminoallyl-modified nucleotides during cDNA synthesis and then
conjugating an N-hydroxysuccinimide (NHS)-ester dye to the
aminoallyl-modified cDNA after the reverse transcription reaction
is complete. Alternatively, the probe may be unlabelled, but may be
detectable by specific binding with a ligand which is labelled,
either directly or indirectly. Suitable labels and methods for
labelling ligands (and probes) are known in the art, and include,
for example, radioactive labels which may be incorporated by known
methods (e.g., nick translation or kinasing). Other suitable labels
include but are not limited to biotin, fluorescent groups,
chemiluminescent groups (e.g., dioxetanes, particularly triggered
dioxetanes), enzymes, antibodies, and the like.
[0129] To perform differential gene expression analysis, cDNA
generated from different RNA samples are labelled with Cy.RTM.3.
The resulting labelled cDNA is purified to remove unincorporated
nucleotides, free dye and residual RNA. Following purification, the
labelled cDNA samples are hybridised to the microarray. The
stringency of hybridisation is determined by a number of factors
during hybridisation and during the washing procedure, including
temperature, ionic strength, length of time and concentration of
formamide. These factors are outlined in, for example, Sambrook et
al. (Molecular Cloning: A Laboratory Manual, 2nd ed., 1989). The
microarray is scanned post-hybridisation using a fluorescent
microarray scanner. The fluorescent intensity of each spot
indicates the level of expression of the analysed gene; bright
spots correspond to strongly expressed genes, while dim spots
indicate weak expression.
[0130] Once the images are obtained, the raw data must be analysed.
First, the background fluorescence must be subtracted from the
fluorescence of each spot. The data is then normalized to a control
sequence, such as exogenously added nucleic acids (preferably RNA
or DNA), or a housekeeping gene panel to account for any
non-specific hybridisation, array imperfections or variability in
the array set-up, cDNA labelling, hybridisation or washing. Data
normalization allows the results of multiple arrays to be
compared.
[0131] Another aspect of the invention relates to a kit for use in
diagnosis of cancer in a subject according to the methods of the
present invention, said kit comprising: a means for measuring the
level of transcription of genes or genomic sequences selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively). In a preferred
embodiment, the means for measuring the level of transcription
comprise oligonucleotides or polynucleotides able to hybridise
under stringent or moderately stringent conditions to the
transcription products of a gene or genomic sequence selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively). Preferably said
oligonucleotides or polynucleotides are able to hybridise under
stringent or moderately stringent conditions to at least one of the
transcription products of a gene or genomic sequence selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively). In one embodiment
said oligonucleotides or polynucleotides comprise at least 9, 18 or
25 bases of a sequence complementary to or hybridising to at least
one sequence selected from the group consisting of SEQ ID NOS:254,
256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280,
282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306,
308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332 and
334 and sequences complementary thereto.
[0132] In a most preferred embodiment, the level of transcription
is determined by techniques selected from the group of Northern
Blot analysis, reverse transcriptase PCR, real-time PCR, RNAse
protection, and microarray. In another embodiment of the invention
the kit further comprises means for obtaining a biological sample
of the patient. Preferred is a kit, which further comprises a
container which is most preferably suitable for containing the
means for measuring the level of transcription and the biological
sample of the patient, and most preferably further comprises
instructions for use and interpretation of the kit results.
[0133] In a preferred embodiment the kit comprises (a) a plurality
of oligonucleotides or polynucleotides able to hybridise under
stringent or moderately stringent conditions to the transcription
products of at least one gene or genomic sequence selected from the
group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively); (b) a container,
preferably suitable for containing the oligonucleotides or
polynucleotides and a biological sample of the patient comprising
the transcription products wherein the oligonucleotides or
polynucleotides can hybridise under stringent or moderately
stringent conditions to the transcription products, (c) means to
detect the hybridisation of (b); and optionally, (d) instructions
for use and interpretation of the kit results. It is further
preferred that said oligonucleotides or polynucleotides of (a)
comprise in each case at least 9, 18 or 25 bases of a sequence
complementary to or hybridising to at least one sequence selected
from the group consisting of SEQ ID NOS:254, 256, 258, 260, 262,
264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288,
290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314,
316, 318, 320, 322, 324, 326, 328, 330, 332 and 334 and sequences
complementary thereto.
[0134] The kit may also contain other components such as
hybridisation buffer (where the oligonucleotides are to be used as
a probe) packaged in a separate container. Alternatively, where the
oligonucleotides are to be used to amplify a target region, the kit
may contain, packaged in separate containers, a polymerase and a
reaction buffer optimised for primer extension mediated by the
polymerase, such as PCR. Preferably said polymerase is a reverse
transcriptase. It is further preferred that said kit further
contains an Rnase reagent.
[0135] The present invention further provides for methods for the
detection of the presence of the polypeptide encoded by said gene
sequences in a sample obtained from a patient.
[0136] Aberrant levels of polypeptide expression of the
polypeptides encoded by the genes or genomic sequences selected
from the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1,
DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table
6); e.g., within SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively) are
associated with CIMP and/or the presence of colon cancer.
[0137] According to the present invention, under expression of said
polypeptides is associated with the presence of CIMP and/or the
presence of colon cancer. It is particularly preferred that said
polypeptides are according to at least one of the amino acid
sequences provided in SEQ ID NOS:255, 257, 259, 261, 263, 265, 267,
269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293,
295, 297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319,
321, 323, 325, 327, 329, 331, 333 and 335 polypeptides transcribed
from the BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1,
IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6) genes).
[0138] Any method known in the art for detecting polypeptides can
be used. Such methods include, but are not limited to
mass-spectrometry, immunodiffusion, immunoelectrophoresis,
immunochemical methods, binder-ligand assays, immunohistochemical
techniques, agglutination and complement assays (e.g., see Basic
and Clinical Immunology, Sites and Terr, eds., Appleton &
Lange, Norwalk, Conn. pp 217-262, 1991 which is incorporated by
reference). Preferred are binder-ligand immunoassay methods
including reacting antibodies with an epitope or epitopes and
competitively displacing a labelled polypeptide or derivative
thereof.
[0139] Certain embodiments of the present invention comprise the
use of antibodies specific to the polypeptide encoded by a gene or
genomic sequence selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively). It is particularly preferred that said polypeptides
are according to at least one of the amino acid sequences provided
in SEQ ID NOS:255, 257, 259, 261, 263, 265, 267, 269, 271, 273,
275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299,
301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325,
327, 329, 331, 333 and 335.
[0140] Such antibodies are useful for CIMP and/or cancer diagnosis.
In certain embodiments production of monoclonal or polyclonal
antibodies can be induced by the use of an epitope encoded by a
polypeptide of SEQ ID NOS:255, 257, 259, 261, 263, 265, 267, 269,
271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295,
297, 299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321,
323, 325, 327, 329, 331, 333 and 335 as an antigene. Such
antibodies may in turn be used to detect expressed polypeptides as
markers for CIMP and/or cancer diagnosis. The levels of such
polypeptides present may be quantified by conventional methods.
Antibody-polypeptide binding may be detected and quantified by a
variety of means known in the art, such as labelling with
fluorescent or radioactive ligands. The invention further comprises
kits for performing the above-mentioned procedures, wherein such
kits contain antibodies specific for the investigated
polypeptides.
[0141] Numerous competitive and non-competitive polypeptide binding
immunoassays are well known in the art. Antibodies employed in such
assays may be unlabelled, for example as used in agglutination
tests, or labelled for use a wide variety of assay methods. Labels
that can be used include radionuclides, enzymes, fluorescers,
chemiluminescers, enzyme substrates or co-factors, enzyme
inhibitors, particles, dyes and the like. Preferred assays include
but are not limited to radioimmunoassay (RIA), enzyme immunoassays,
e.g., enzyme-linked immunosorbent assay (ELISA), fluorescent
immunoassays and the like. Polyclonal or monoclonal antibodies or
epitopes thereof can be made for use in immunoassays by any of a
number of methods known in the art.
[0142] In an alternative embodiment of the method the proteins may
be detected by means of western blot analysis. Said analysis is
standard in the art, briefly proteins are separated by means of
electrophoresis, e.g., SDS-PAGE. The separated proteins are then
transferred to a suitable membrane (or paper), e.g.,
nitrocellulose, retaining the spacial separation achieved by
electrophoresis. The membrane is then incubated with a blocking
agent to bind remaining sticky places on the membrane, commonly
used agents include generic protein (e.g., milk protein). An
antibody specific to the protein of interest is then added, said
antibody being detectably labelled for example by dyes or enzymatic
means (e.g., alkaline phosphatase or horseradish peroxidase). The
location of the antibody on the membrane is then detected.
[0143] In an alternative embodiment of the method the proteins may
be detected by means of immunohistochemistry (the use of antibodies
to probe specific antigens in a sample). Said analysis is standard
in the art, wherein detection of antigens in tissues is known as
immunohistochemistry, while detection in cultured cells is
generally termed immunocytochemistry. Briefly, the primary antibody
to be detected by binding to its specific antigen. The
antibody-antigen complex is then bound by a secondary enzyme
conjugated antibody. In the presence of the necessary substrate and
chromogen the bound enzyme is detected according to coloured
deposits at the antibody-antigen binding sites. There is a wide
range of suitable sample types, antigen-antibody affinity, antibody
types, and detection enhancement methods. Thus optimal conditions
for immunohistochemical or immunocytochemical detection must be
determined by the person skilled in the art for each individual
case.
[0144] One approach for preparing antibodies to a polypeptide is
the selection and preparation of an amino acid sequence of all or
part of the polypeptide, chemically synthesising the amino acid
sequence and injecting it into an appropriate animal, usually a
rabbit or a mouse (Milstein and Kohler Nature 256:495-497, 1975;
Gulfre and Milstein, Methods in Enzymology: Immunochemical
Techniques 73:1-46, Langone and Banatis eds., Academic Press, 1981
which are incorporated by reference in its entirety). Methods for
preparation of the polypeptides or epitopes thereof include, but
are not limited to chemical synthesis, recombinant DNA techniques
or isolation from biological samples.
[0145] In the final step of the method the diagnosis of the patient
is determined, whereby under-expression (of at least one gene or
genomic sequence selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) is indicative of the presence of CIMP and/or cancer.
The term under-expression shall be taken to mean expression at a
detected level less than a pre-determined cut off which may be
selected from the group consisting of the mean, median or an
optimised threshold value.
[0146] Another aspect of the invention provides a kit for use in
diagnosis of CIMP and/or cancer in a subject according to the
methods of the present invention, comprising: a means for detecting
polypeptides at least one gene or genomic sequence selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively). Preferably the
sequence of said polypeptides is as provided in SEQ ID NOS:255,
257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281,
283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303, 305, 307,
309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329, 331, 333 and
335. The means for detecting the polypeptides comprise preferably
antibodies, antibody derivatives, or antibody fragments. The
polypeptides are most preferably detected by means of Western
Blotting utilizing a labelled antibody. In another embodiment of
the invention the kit further comprising means for obtaining a
biological sample of the patient. Preferred is a kit, which further
comprises a container suitable for containing the means for
detecting the polypeptides in the biological sample of the patient,
and most preferably further comprises instructions for use and
interpretation of the kit results. In a preferred embodiment the
kit comprises: (a) a means for detecting polypeptides at least one
gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively); (b) a container suitable for
containing the said means and the biological sample of the patient
comprising the polypeptides wherein the means can form complexes
with the polypeptides; (c) a means to detect the complexes of (b);
and optionally (d) instructions for use and interpretation of the
kit results. It is preferred that said means for detecting
polypeptides of at least one gene or genomic sequence selected from
the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6);
e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively) are specific for
at least one of the polypeptide sequences selected from SEQ ID
NOS:255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277,
279, 281, 283, 285, 287, 289, 291, 293, 295, 297, 299, 301, 303,
305, 307, 309, 311, 313, 315, 317, 319, 321, 323, 325, 327, 329,
331, 333 and 335. The kit may also contain other components such as
buffers or solutions suitable for blocking, washing or coating,
packaged in a separate container.
[0147] Particular embodiments of the present invention provide a
novel application of the analysis of methylation levels and/or
patterns within said sequences that enables a precise detection,
characterisation and/or treatment of CIMP and/or colorectal cell
proliferative disorders. Early detection of CIMP is directly linked
with disease prognosis, and the disclosed method thereby enables
the physician and patient to make better and more informed
treatment decisions.
Further Improvements
[0148] The present invention provides novel compositions and uses
as disclosed herein for genomic (e.g., CpG island) markers
corresponding to at least one of BCL2, BDNF, CACNA1G, CALCA,
CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1
(Table 6) sequences corresponding to e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively). Additional embodiments provide
modified variants of e.g., within SEQ ID NOS:128-141, 114-127 and
100-113 (respective genomic, CpG island and amplicons,
respectively), as well as oligonucleotides and/or PNA-oligomers for
analysis of cytosine methylation patterns within e.g., within SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively).
[0149] An objective of the invention comprises analysis of the
methylation state of one or more CpG dinucleotides within at least
one sequence selected form the group consisting of e.g., within SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) and sequences complementary
thereto.
[0150] The disclosed invention provides treated nucleic acids,
derived from genomic e.g., within SEQ ID NOS:128-141, 114-127 and
100-113 (respective genomic, CpG island and amplicons,
respectively), wherein the treatment is suitable to convert at
least one unmethylated cytosine base of the genomic DNA sequence to
uracil or another base that is detectably dissimilar to cytosine in
terms of hybridization. The genomic sequences in question may
comprise one, or more consecutive methylated CpG positions. Said
treatment preferably comprises use of a reagent selected from the
group consisting of bisulfate, hydrogen sulfite, disulfite, and
combinations thereof. In a preferred embodiment of the invention,
the invention provides a non-naturally occurring modified nucleic
acid comprising a sequence of at least 16 contiguous nucleotide
bases in length of a sequence selected from the group consisting of
SEQ ID NOS:170-197, 226-253, 142-169 and 198-225. In further
preferred embodiments of the invention said nucleic acid is at
least 50, 100, 150, 200, 250 or 500 base pairs in length of a
segment of the nucleic acid sequence disclosed in SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225. Particularly preferred
is a nucleic acid molecule that is identical or complementary to
all or a portion of the sequences SEQ ID NOS:170-197, 226-253,
142-169 and 198-225 but not SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively) or
other naturally occurring DNA.
[0151] It is preferred that said sequence comprises at least one
CpG, TpA or CpA dinucleotide and sequences complementary thereto.
The sequences of SEQ ID NOS:170-197, 226-253, 142-169 and 198-225
provide non-naturally occurring modified versions of the nucleic
acid according to SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively),
wherein the modification of each genomic sequence results in the
synthesis of a nucleic acid having a sequence that is unique and
distinct from said genomic sequence as follows. For each sense
strand genomic DNA, e.g., SEQ ID NO:1, four converted versions are
disclosed. A first version wherein "C" is converted to "T," but
"CpG" remains "CpG" (i.e., corresponds to case where, for the
genomic sequence, all "C" residues of CpG dinucleotide sequences
are methylated and are thus not converted); a second version
discloses the complement of the disclosed genomic DNA sequence
(i.e. antisense strand), wherein "C" is converted to "T," but "CpG"
remains "CpG" (i.e., corresponds to case where, for all "C"
residues of CpG dinucleotide sequences are methylated and are thus
not converted). The `upmethylated` converted sequences of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) correspond to SEQ ID NOS:170-197 and
142-169. A third chemically converted version of each genomic
sequences is provided, wherein "C" is converted to "T" for all "C"
residues, including those of "CpG" dinucleotide sequences (i.e.,
corresponds to case where, for the genomic sequences, all "C"
residues of CpG dinucleotide sequences are unmethylated); a final
chemically converted version of each sequence, discloses the
complement of the disclosed genomic DNA sequence (i.e. antisense
strand), wherein "C" is converted to "T" for all "C" residues,
including those of "CpG" dinucleotide sequences (i.e., corresponds
to case where, for the complement (antisense strand) of each
genomic sequence, all "C" residues of CpG dinucleotide sequences
are unmethylated). The `downmethylated` converted sequences of SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) correspond to SEQ ID NOS:226-253 and
198-225.
[0152] Significantly, heretofore, the nucleic acid sequences and
molecules according SEQ ID NOS:170-197, 226-253, 142-169 and
198-225 were not implicated in or connected with the detection,
classification or treatment of cellular proliferative
disorders.
[0153] In an alternative preferred embodiment, the invention
further provides oligonucleotides or oligomers suitable for use in
the methods of the invention for detecting the cytosine methylation
state within genomic or treated (chemically modified) DNA,
according to SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively), SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225. Said oligonucleotide or
oligomer nucleic acids provide novel diagnostic means. Said
oligonucleotide or oligomer comprising a nucleic acid sequence
having a length of at least nine (9) nucleotides which is identical
to, hybridizes, under moderately stringent or stringent conditions
(as defined herein above), to a treated nucleic acid sequence
according to SEQ ID NOS:170-197, 226-253, 142-169 and 198-225
and/or sequences complementary thereto, or to a genomic sequence
according to SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively) and/or sequences
complementary thereto.
[0154] Thus, the present invention includes nucleic acid molecules
(e.g., oligonucleotides and peptide nucleic acid (PNA) molecules
(PNA-oligomers)) that hybridize under moderately stringent and/or
stringent hybridization conditions to all or a portion of a
sequence selected form the group consisting SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively), SEQ ID NOS:170-197, 226-253, 142-169 and 198-225 or
to the complements thereof. Particularly preferred is a nucleic
acid molecule that hybridizes under moderately stringent and/or
stringent hybridization conditions to all or a portion of the
sequences SEQ ID NOS:170-197, 226-253, 142-169 and 198-225 but not
SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG
island and amplicons, respectively) or other human genomic DNA.
[0155] The identical or hybridizing portion of the hybridizing
nucleic acids is typically at least 9, 16, 20, 25, 30 or 35
nucleotides in length. However, longer molecules have inventive
utility, and are thus within the scope of the present
invention.
[0156] Preferably, the hybridizing portion of the inventive
hybridizing nucleic acids is at least 95%, or at least 98%, or 100%
identical to the sequence, or to a portion thereof of a sequence
selected from the group consisting of SEQ ID NOS:128-141, 114-127
and 100-113 (respective genomic, CpG island and amplicons,
respectively), SEQ ID NOS:170-197, 226-253, 142-169 and 198-225, or
to the complements thereof.
[0157] Hybridizing nucleic acids of the type described herein can
be used, for example, as a primer (e.g., a PCR primer), or a
diagnostic and/or prognostic probe or primer. Preferably,
hybridization of the oligonucleotide probe to a nucleic acid sample
is performed under stringent conditions and the probe is 100%
identical to the target sequence. Nucleic acid duplex or hybrid
stability is expressed as the melting temperature or Tm, which is
the temperature at which a probe dissociates from a target DNA.
This melting temperature is used to define the required stringency
conditions.
[0158] For target sequences that are related and substantially
identical to the corresponding sequence of SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) (such as allelic variants and SNPs), rather than
identical, it is useful to first establish the lowest temperature
at which only homologous hybridization occurs with a particular
concentration of salt (e.g., SSC or SSPE). Then, assuming that 1%
mismatching results in a 1.degree. C. decrease in the Tm, the
temperature of the final wash in the hybridization reaction is
reduced accordingly (for example, if sequences having >95%
identity with the probe are sought, the final wash temperature is
decreased by 5.degree. C.). In practice, the change in Tm can be
between 0.5.degree. C. and 1.5.degree. C. per 1% mismatch.
[0159] Examples of inventive oligonucleotides of length X (in
nucleotides), as indicated by polynucleotide positions with
reference to, e.g., (NEUROG1 CpG Island; SEQ ID NO:124, include
those corresponding to sets (sense and antisense sets) of
consecutively overlapping oligonucleotides of length X, where the
oligonucleotides within each consecutively overlapping set
(corresponding to a given X value) are defined as the finite set of
Z oligonucleotides from nucleotide positions:
[0160] n to (n+(X-1));
[0161] where n=1, 2, 3, . . . (Y-(X-1));
[0162] where Y equals the length (nucleotides or base pairs) of SEQ
ID NO:124 (2,091);
[0163] where X equals the common length (in nucleotides) of each
oligonucleotide in the set (e.g., X=20 for a set of consecutively
overlapping 20-mers); and
[0164] where the number (Z) of consecutively overlapping oligomers
of length X for a given SEQ ID NO of length Y is equal to Y-(X-1).
For example Z=2,091-19=2,072 for either sense or antisense sets of
SEQ ID NO: 124, where X=20.
[0165] Preferably, the set is limited to those oligomers that
comprise at least one CpG, TpG or CpA dinucleotide.
[0166] Examples of inventive 20-mer oligonucleotides include the
following set of 2,072 oligomers (and the antisense set
complementary thereto), indicated by polynucleotide positions with
reference to SEQ ID NO: 124:
[0167] 1-20, 2-21, 3-22, 4-23, 5-24, . . . and 2,072-2,091.
[0168] Preferably, the set is limited to those oligomers that
comprise at least one CpG, TpG or CpA dinucleotide.
[0169] Likewise, examples of inventive 25-mer oligonucleotides
include the following set of 2,067 oligomers (and the antisense set
complementary thereto), indicated by polynucleotide positions with
reference to SEQ ID NO: 124:
[0170] 1-25, 2-26, 3-27, 4-28, 5-29, . . . and 2,067-2,091.
[0171] Preferably, the set is limited to those oligomers that
comprise at least one CpG, TpG or CpA dinucleotide.
[0172] The present invention encompasses, for each of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively), SEQ ID NOS:170-197, 226-253, 142-169
and 198-225 (sense and antisense), multiple consecutively
overlapping sets of oligonucleotides or modified oligonucleotides
of length X, where, e.g., X=9, 10, 17, 20, 22, 23, 25, 27, 30 or 35
nucleotides.
[0173] The oligonucleotides or oligomers according to the present
invention constitute effective tools useful to ascertain genetic
and epigenetic parameters of the genomic sequences selected from
the group consisting of SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively).
Preferred sets of such oligonucleotides or modified
oligonucleotides of length X are those consecutively overlapping
sets of oligomers corresponding to SEQ ID NOS:128-141, 114-127 and
100-113 (respective genomic, CpG island and amplicons,
respectively), SEQ ID NOS:170-197, 226-253, 142-169 and 198-225
(and to the complements thereof). Preferably, said oligomers
comprise at least one CpG, TpG or CpA dinucleotide.
[0174] Particularly preferred oligonucleotides or oligomers
according to the present invention are those in which the cytosine
of the CpG dinucleotide (or of the corresponding converted TpG or
CpA dinculeotide) sequences is within the middle third of the
oligonucleotide; that is, where the oligonucleotide is, for
example, 13 bases in length, the CpG, TpG or CpA dinucleotide is
positioned within the fifth to ninth nucleotide from the
5'-end.
[0175] The oligonucleotides of the invention can also be modified
by chemically linking the oligonucleotide to one or more moieties
or conjugates to enhance the activity, stability or detection of
the oligonucleotide. Such moieties or conjugates include
chromophores, fluorophors, lipids such as cholesterol, cholic acid,
thioether, aliphatic chains, phospholipids, polyamines,
polyethylene glycol (PEG), palmityl moieties, and others as
disclosed in, for example, U.S. Pat. Nos. 5,514,758, 5,565,552,
5,567,810, 5,574,142, 5,585,481, 5,587,371, 5,597,696 and
5,958,773. The probes may also exist in the form of a PNA (peptide
nucleic acid) which has particularly preferred pairing properties.
Thus, the oligonucleotide may include other appended groups such as
peptides, and may include hybridization-triggered cleavage agents
(Krol et al., BioTechniques 6:958-976, 1988) or intercalating
agents (Zon, Pharm. Res. 5:539-549, 1988). To this end, the
oligonucleotide may be conjugated to another molecule, e.g., a
chromophore, fluorophor, peptide, hybridization-triggered
cross-linking agent, transport agent, hybridization-triggered
cleavage agent, etc.
[0176] The oligonucleotide may also comprise at least one
art-recognized modified sugar and/or base moiety, or may comprise a
modified backbone or non-natural internucleoside linkage.
[0177] The oligonucleotides or oligomers according to particular
embodiments of the present invention are typically used in `sets,`
which contain at least one oligomer for analysis of each of the CpG
dinucleotides of a genomic sequence selected from the group
consisting of SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively) and sequences
complementary thereto, or to the corresponding CpG, TpG or CpA
dinucleotide within a sequence of the treated nucleic acids
according to SEQ ID NOS:170-197, 226-253, 142-169 and 198-225 and
sequences complementary thereto. However, it is anticipated that
for economic or other factors it may be preferable to analyse a
limited selection of the CpG dinucleotides within said sequences,
and the content of the set of oligonucleotides is altered
accordingly.
[0178] Therefore, in particular embodiments, the present invention
provides a set of at least two (2) (oligonucleotides and/or
PNA-oligomers) useful for detecting the cytosine methylation state
in treated genomic DNA (SEQ ID NOS:170-197, 226-253, 142-169 and
198-225), or in genomic DNA (e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) and sequences complementary thereto). These probes
enable diagnosis, classification and/or therapy of genetic and
epigenetic parameters of liver and/or colorectal cell proliferative
disorders. The set of oligomers may also be used for detecting
single nucleotide polymorphisms (SNPs) in treated genomic DNA (SEQ
ID NOS:170-197, 226-253, 142-169 and 198-225), or in genomic DNA
(e.g., within SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively) and sequences
complementary thereto).
[0179] In preferred embodiments, at least one, and more preferably
all members of a set of oligonucleotides is bound to a solid
phase.
[0180] In further embodiments, the present invention provides a set
of at least two (2) oligonucleotides that are used as `primer`
oligonucleotides for amplifying DNA sequences of one of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively), SEQ ID NOS:170-197, 226-253, 142-169
and 198-225 and sequences complementary thereto, or segments
thereof.
[0181] It is anticipated that the oligonucleotides may constitute
all or part of an "array" or "DNA chip" (i.e., an arrangement of
different oligonucleotides and/or PNA-oligomers bound to a solid
phase). Such an array of different oligonucleotide- and/or
PNA-oligomer sequences can be characterized, for example, in that
it is arranged on the solid phase in the form of a rectangular or
hexagonal lattice. The solid-phase surface may be composed of
silicon, glass, polystyrene, aluminium, steel, iron, copper,
nickel, silver, or gold. Nitrocellulose as well as plastics such as
nylon, which can exist in the form of pellets or also as resin
matrices, may also be used. An overview of the Prior Art in
oligomer array manufacturing can be gathered from a special edition
of Nature Genetics (Nature Genetics Supplement, Volume 21, January
1999, and from the literature cited therein). Fluorescently
labelled probes are often used for the scanning of immobilized DNA
arrays. The simple attachment of Cy3 and Cy5 dyes to the 5'-OH of
the specific probe are particularly suitable for fluorescence
labels. The detection of the fluorescence of the hybridised probes
may be carried out, for example, via a confocal microscope. Cy3 and
Cy5 dyes, besides many others, are commercially available.
[0182] It is also anticipated that the oligonucleotides, or
particular sequences thereof, may constitute all or part of an
"virtual array" wherein the oligonucleotides, or particular
sequences thereof, are used, for example, as `specifiers` as part
of, or in combination with a diverse population of unique labeled
probes to analyze a complex mixture of analytes. Such a method, for
example is described in US 2003/0013091 (U.S. Ser. No. 09/898,743,
published 16 Jan. 2003). In such methods, enough labels are
generated so that each nucleic acid in the complex mixture (i.e.,
each analyte) can be uniquely bound by a unique label and thus
detected (each label is directly counted, resulting in a digital
read-out of each molecular species in the mixture).
[0183] It is particularly preferred that the oligomers according to
the invention are utilised for at least one of: determining and/or
classifying CIMP status in colorectal cancers; identifying KRAS
mutant, BRAF wildtype tumors; determining the relationship between
CIMP status and other molecular features of the cancers including,
but not limited to BRAF mutation, KRAS mutation and MSI status;
determining the relationship between CIMP status and other
variables including, but not limited to age, sex, tumor location,
family history, race, country of origin, tumor characteristics
(including, tumor type, tumor grade, invasive margin
characteristics, lymphocyte infiltration characteristics, direct
spread, lymph node spread, venous spread and type of residual
adjacent polyp, if present); determining, between subgroups defined
by CIMP status and BRAF mutations, effects of selected risk factors
including, but not limited to body mass index, smoking history,
alcohol intake, dietary folate intake, folate metabolic enzyme
polymorphisms and history of hormonal use; and providing a
foundation for a population-based study of CIMP, by providing a
novel panel of carefully selected methylation markers representing
the CIMP subgroup, and having utility to classify CIMP.
[0184] In particular aspects, this is enabled by use of said sets
for the detection or detection and differentiation of one or more
of the following classes of tissues: colorectal carcinoma, colon
adenoma, inflammatory colon tissue, grade 2 dysplasia colon
adenomas less than 1 cm, grade 3 dysplasia colon adenomas larger
than 1 cm, normal colon tissue, non-colon healthy tissue and
non-colon cancer tissue.
[0185] Particularly preferred are those sets of oligomers according
to the Examples.
[0186] In the most preferred embodiment of the method, CIMP status
is determined or classified. This is achieved by analysis of the
methylation status of at least one target sequence comprising at
least one CpG position said sequence comprising, or hybridizing
under stringent conditions to at least 16 contiguous nucleotides of
a sequence selected from the group consisting of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) and complements thereof. The present
invention further provides a method for ascertaining genetic and/or
epigenetic parameters of the genomic sequence according to SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) within a subject by analysing cytosine
methylation and single nucleotide polymorphisms. Said method
comprising contacting a nucleic acid comprising e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) in a biological sample obtained from
said subject with at least one reagent or a series of reagents,
wherein said reagent or series of reagents, distinguishes between
methylated and non-methylated CpG dinucleotides within the target
nucleic acid.
[0187] In a preferred embodiment, said method comprises the
following steps: In the first step, a sample of the tissue to be
analysed is obtained. The source may be any suitable source, such
as cell lines, histological slides, biopsies, paraffin-embedded
tissue, body fluids, stool, colonic effluent, urine, blood plasma,
blood serum, whole blood, isolated blood cells, cells isolated from
the blood and all possible combinations thereof. It is preferred
that said sources of DNA are stool or body fluids selected from the
group consisting colonic effluent, urine, blood plasma, blood
serum, whole blood, isolated blood cells, cells isolated from the
blood.
[0188] The genomic DNA is then isolated from the sample. Genomic
DNA may be isolated by any means standard in the art, including the
use of commercially available kits. Briefly, wherein the DNA of
interest is encapsulated in by a cellular membrane the biological
sample must be disrupted and lysed by enzymatic, chemical or
mechanical means. The DNA solution may then be cleared of proteins
and other contaminants e.g. by digestion with proteinase K. The
genomic DNA is then recovered from the solution. This may be
carried out by means of a variety of methods including salting out,
organic extraction or binding of the DNA to a solid phase support.
The choice of method will be affected by several factors including
time, expense and required quantity of DNA.
[0189] Wherein the sample DNA is not enclosed in a membrane (e.g.
circulating DNA from a blood sample) methods standard in the art
for the isolation and/or purification of DNA may be employed. Such
methods include the use of a protein degenerating reagent e.g.,
chaotropic salt e.g. guanidine hydrochloride or urea; or a
detergent e.g. sodium dodecyl sulphate (SDS), cyanogen bromide.
Alternative methods include but are not limited to ethanol
precipitation or propanol precipitation, vacuum concentration
amongst others by means of a centrifuge. The person skilled in the
art may also make use of devices such as filter devices, e.g.,
ultrafiltration, silica surfaces or membranes, magnetic particles,
polystyrol particles, polystyrol surfaces, positively charged
surfaces, and positively charged membranse, charged membranes,
charged surfaces, charged switch membranes, charged switched
surfaces.
[0190] Once the nucleic acids have been extracted, the genomic
double stranded DNA is used in the analysis.
[0191] In the second step of the method, the genomic DNA sample is
treated in such a manner that cytosine bases which are unmethylated
at the 5'-position are converted to uracil, thymine, or another
base which is dissimilar to cytosine in terms of hybridisation
behaviour. This will be understood as `pre-treatment` or
`treatment` herein.
[0192] This is preferably achieved by means of treatment with a
bisulfite reagent. The term "bisulfite reagent" refers to a reagent
comprising bisulfite, disulfite, hydrogen sulfite or combinations
thereof, useful as disclosed herein to distinguish between
methylated and unmethylated CpG dinucleotide sequences. Methods of
said treatment are known in the art (e.g., PCT/EP2004/011715, which
is incorporated by reference in its entirety). It is preferred that
the bisulfite treatment is conducted in the presence of denaturing
solvents such as but not limited to n-alkylenglycol, particularly
diethylene glycol dimethyl ether (DME), or in the presence of
dioxane or dioxane derivatives. In a preferred embodiment the
denaturing solvents are used in concentrations between 1% and 35%
(v/v). It is also preferred that the bisulfite reaction is carried
out in the presence of scavengers such as but not limited to
chromane derivatives, e.g., 6-hydroxy-2,5,7,8,-tetramethylchromane
2-carboxylic acid or trihydroxybenzone acid and derivates thereof,
e.g., Gallic acid (see: PCT/EP2004/011715 which is incorporated by
reference in its entirety). The bisulfite conversion is preferably
carried out at a reaction temperature between 30.degree. C. and
70.degree. C., whereby the temperature is increased to over
85.degree. C. for short periods of times during the reaction (see:
PCT/EP2004/011715 which is incorporated by reference in its
entirety). The bisulfite treated DNA is preferably purified priori
to the quantification. This may be conducted by any means known in
the art, such as but not limited to ultrafiltration, preferably
carried out by means of Microcon.TM. columns (manufactured by
Millipore.TM.). The purification is carried out according to a
modified manufacturer's protocol (see: PCT/EP2004/011715 which is
incorporated by reference in its entirety).
[0193] In the third step of the method, fragments of the treated
DNA are amplified, using sets of primer oligonucleotides according
to the present invention, and an amplification enzyme. The
amplification of several DNA segments can be carried out
simultaneously in one and the same reaction vessel. Typically, the
amplification is carried out using a polymerase chain reaction
(PCR). Preferably said amplificates are 100 to 2,000 base pairs in
length. The set of primer oligonucleotides includes at least two
oligonucleotides whose sequences are each reverse complementary,
identical, or hybridise under stringent or highly stringent
conditions to an at least 16-base-pair long segment of the base
sequences of one of SEQ ID NOS:170-197, 226-253, 142-169 and
198-225 and sequences complementary thereto.
[0194] In an alternate embodiment of the method, the methylation
status of pre-selected CpG positions within at least one nucleic
acid sequences selected from the group consisting of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) may be detected by use of
methylation-specific primer oligonucleotides. This technique (MSP)
has been described in U.S. Pat. No. 6,265,171 to Herman. The use of
methylation status specific primers for the amplification of
bisulfite treated DNA allows the differentiation between methylated
and unmethylated nucleic acids. MSP primers pairs contain at least
one primer which hybridises to a bisulfite treated CpG
dinucleotide. Therefore, the sequence of said primers comprises at
least one CpG dinucleotide. MSP primers specific for non-methylated
DNA contain a "T" at the position of the C position in the CpG.
Preferably, therefore, the base sequence of said primers is
required to comprise a sequence having a length of at least 9
nucleotides which hybridises to a treated nucleic acid sequence
according to one of SEQ ID NOS:170-197, 226-253, 142-169 and
198-225 and sequences complementary thereto, wherein the base
sequence of said oligomers comprises at least one CpG dinucleotide.
A further preferred embodiment of the method comprises the use of
blocker oligonucleotides (the HeavyMethyl.TM. assay). The use of
such blocker oligonucleotides has been described by Yu et al.,
BioTechniques 23:714-720, 1997. Blocking probe oligonucleotides are
hybridised to the bisulfite treated nucleic acid concurrently with
the PCR primers. PCR amplification of the nucleic acid is
terminated at the 5' position of the blocking probe, such that
amplification of a nucleic acid is suppressed where the
complementary sequence to the blocking probe is present. The probes
may be designed to hybridize to the bisulfite treated nucleic acid
in a methylation status specific manner. For example, for detection
of methylated nucleic acids within a population of unmethylated
nucleic acids, suppression of the amplification of nucleic acids
which are unmethylated at the position in question would be carried
out by the use of blocking probes comprising a `CpA` or `TpA` at
the position in question, as opposed to a `CpG` if the suppression
of amplification of methylated nucleic acids is desired.
[0195] For PCR methods using blocker oligonucleotides, efficient
disruption of polymerase-mediated amplification requires that
blocker oligonucleotides not be elongated by the polymerase.
Preferably, this is achieved through the use of blockers that are
3'-deoxyoligonucleotides, or oligonucleotides derivitized at the 3'
position with other than a "free" hydroxyl group. For example,
3'-O-acetyl oligonucleotides are representative of a preferred
class of blocker molecule.
[0196] Additionally, polymerase-mediated decomposition of the
blocker oligonucleotides should be precluded. Preferably, such
preclusion comprises either use of a polymerase lacking 5'-3'
exonuclease activity, or use of modified blocker oligonucleotides
having, for example, thioate bridges at the 5'-terminii thereof
that render the blocker molecule nuclease-resistant. Particular
applications may not require such 5' modifications of the blocker.
For example, if the blocker- and primer-binding sites overlap,
thereby precluding binding of the primer (e.g., with excess
blocker), degradation of the blocker oligonucleotide will be
substantially precluded. This is because the polymerase will not
extend the primer toward, and through (in the 5'-3' direction) the
blocker--a process that normally results in degradation of the
hybridized blocker oligonucleotide.
[0197] A particularly preferred blocker/PCR embodiment, for
purposes of the present invention and as implemented herein,
comprises the use of peptide nucleic acid (PNA) oligomers as
blocking oligonucleotides. Such PNA blocker oligomers are ideally
suited, because they are neither decomposed nor extended by the
polymerase.
[0198] Preferably, therefore, the base sequence of said blocking
oligonucleotides is required to comprise a sequence having a length
of at least 9 nucleotides which hybridises to a treated nucleic
acid sequence according to one of SEQ ID NOS:170-197, 226-253,
142-169 and 198-225 and sequences complementary thereto, wherein
the base sequence of said oligonucleotides comprises at least one
CpG, TpG or CpA dinucleotide.
[0199] The fragments obtained by means of the amplification can
carry a directly or indirectly detectable label. Preferred are
labels in the form of fluorescence labels, radionuclides, or
detachable molecule fragments having a typical mass which can be
detected in a mass spectrometer. Where said labels are mass labels,
it is preferred that the labelled amplificates have a single
positive or negative net charge, allowing for better delectability
in the mass spectrometer. The detection may be carried out and
visualized by means of, e.g., matrix assisted laser
desorption/ionization mass spectrometry (MALDI) or using electron
spray mass spectrometry (ESI).
[0200] Matrix Assisted Laser Desorption/Ionization Mass
Spectrometry (MALDI-TOF) is a very efficient development for the
analysis of biomolecules (Karas & Hillenkamp, Anal Chem.,
60:2299-301, 1988). An analyte is embedded in a light-absorbing
matrix. The matrix is evaporated by a short laser pulse thus
transporting the analyte molecule into the vapor phase in an
unfragmented manner. The analyte is ionized by collisions with
matrix molecules. An applied voltage accelerates the ions into a
field-free flight tube. Due to their different masses, the ions are
accelerated at different rates. Smaller ions reach the detector
sooner than bigger ones. MALDI-TOF spectrometry is well suited to
the analysis of peptides and proteins. The analysis of nucleic
acids is somewhat more difficult (Gut & Beck, Current
Innovations and Future Trends, 1:147-57, 1995). The sensitivity
with respect to nucleic acid analysis is approximately 100-times
less than for peptides, and decreases disproportionally with
increasing fragment size. Moreover, for nucleic acids having a
multiply negatively charged backbone, the ionization process via
the matrix is considerably less efficient. In MALDI-TOF
spectrometry, the selection of the matrix plays an eminently
important role. For desorption of peptides, several very efficient
matrixes have been found which produce a very fine crystallisation.
There are now several responsive matrixes for DNA, however, the
difference in sensitivity between peptides and nucleic acids has
not been reduced. This difference in sensitivity can be reduced,
however, by chemically modifying the DNA in such a manner that it
becomes more similar to a peptide. For example, phosphorothioate
nucleic acids, in which the usual phosphates of the backbone are
substituted with thiophosphates, can be converted into a
charge-neutral DNA using simple alkylation chemistry (Gut &
Beck, Nucleic Acids Res. 23: 1367-73, 1995). The coupling of a
charge tag to this modified DNA results in an increase in MALDI-TOF
sensitivity to the same level as that found for peptides. A further
advantage of charge tagging is the increased stability of the
analysis against impurities, which makes the detection of
unmodified substrates considerably more difficult.
[0201] In the fourth step of the method, the amplificates obtained
during the third step of the method are analysed in order to
ascertain the methylation status of the CpG dinucleotides prior to
the treatment.
[0202] In embodiments where the amplificates were obtained by means
of MSP amplification, the presence or absence of an amplificate is
in itself indicative of the methylation state of the CpG positions
covered by the primer, according to the base sequences of said
primer.
[0203] Amplificates obtained by means of both standard and
methylation specific PCR may be further analysed by means of
based-based methods such as, but not limited to, array technology
and probe based technologies as well as by means of techniques such
as sequencing and template directed extension.
[0204] In one embodiment of the method, the amplificates
synthesised in step three are subsequently hybridized to an array
or a set of oligonucleotides and/or PNA probes. In this context,
the hybridization takes place in the following manner: the set of
probes used during the hybridization is preferably composed of at
least 2 oligonucleotides or PNA-oligomers; in the process, the
amplificates serve as probes which hybridize to oligonucleotides
previously bonded to a solid phase; the non-hybridized fragments
are subsequently removed; said oligonucleotides contain at least
one base sequence having a length of at least 9 nucleotides which
is reverse complementary or identical to a segment of the base
sequences specified in the present Sequence Listing; and the
segment comprises at least one CpG, TpG or CpA dinucleotide. The
hybridizing portion of the hybridizing nucleic acids is typically
at least 9, 15, 20, 25, 30 or 35 nucleotides in length. However,
longer molecules have inventive utility, and are thus within the
scope of the present invention.
[0205] In a preferred embodiment, said dinucleotide is present in
the central third of the oligomer. For example, wherein the
oligomer comprises one CpG dinucleotide, said dinucleotide is
preferably the fifth to ninth nucleotide from the 5'-end of a
13-mer. One oligonucleotide exists for the analysis of each CpG
dinucleotide within a sequence selected from the group consisting
of SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG
island and amplicons, respectively), and the equivalent positions
within SEQ ID NOS:170-197, 226-253, 142-169 and 198-225.
[0206] Said oligonucleotides may also be present in the form of
peptide nucleic acids. The non-hybridised amplificates are then
removed. The hybridised amplificates are then detected. In this
context, it is preferred that labels attached to the amplificates
are identifiable at each position of the solid phase at which an
oligonucleotide sequence is located.
[0207] In yet a further embodiment of the method, the genomic
methylation status of the CpG positions may be ascertained by means
of oligonucleotide probes (as detailed above) that are hybridised
to the bisulfite treated DNA concurrently with the PCR
amplification primers (wherein said primers may either be
methylation specific or standard).
[0208] A particularly preferred embodiment of this method is the
use of fluorescence-based Real Time Quantitative PCR (Heid et al.,
Genome Res. 6:986-994, 1996; also see U.S. Pat. No. 6,331,393)
employing a dual-labelled fluorescent oligonucleotide probe
(TaqMan.TM. PCR, using an ABI Prism 7700 Sequence Detection System,
Perkin Elmer Applied Biosystems, Foster City, Calif.). The
TaqMan.TM. PCR reaction employs the use of a non-extendible
interrogating oligonucleotide, called a TaqMan.TM. probe, which, in
preferred embodiments, is designed to hybridise to a CpG-rich
sequence located between the forward and reverse amplification
primers. The TaqMan.TM. probe further comprises a fluorescent
"reporter moiety" and a "quencher moiety" covalently bound to
linker moieties (e.g., phosphoramidites) attached to the
nucleotides of the TaqMan.TM. oligonucleotide. For analysis of
methylation within nucleic acids subsequent to bisulfite treatment,
it is required that the probe be methylation specific, as described
in U.S. Pat. No. 6,331,393, (hereby incorporated by reference in
its entirety) also known as the MethyLightTM.TM. assay. Variations
on the TaqMan.TM. detection methodology that are also suitable for
use with the described invention include the use of dual-probe
technology (Lightcycler.TM.) or fluorescent amplification primers
(Sunrise.TM. technology). Both these techniques may be adapted in a
manner suitable for use with bisulfite treated DNA, and moreover
for methylation analysis within CpG dinucleotides.
[0209] In a further preferred embodiment of the method, the fourth
step of the method comprises the use of template-directed
oligonucleotide extension, such as MS-SNuPE as described by
Gonzalgo & Jones, Nucleic Acids Res. 25:2529-2531, 1997.
[0210] In yet a further embodiment of the method, the fourth step
of the method comprises sequencing and subsequent sequence analysis
of the amplificate generated in the third step of the method
(Sanger F., et al., Proc Natl Acad Sci USA 74:5463-5467, 1977).
Best Mode
[0211] In a preferred embodiment of the method, the genomic nucleic
acids are isolated and treated according to the first three steps
of the method outlined above, namely:
[0212] a) obtaining, from a subject, a biological sample having
subject genomic DNA;
[0213] b) extracting or otherwise isolating the genomic DNA;
[0214] c) treating the genomic DNA of b), or a fragment thereof,
with one or more reagents to convert cytosine bases that are
unmethylated in the 5-position thereof to uracil or to another base
that is detectably dissimilar to cytosine in terms of hybridization
properties; and wherein
[0215] d) amplifying subsequent to treatment in c) is carried out
in a methylation specific manner, namely by use of methylation
specific primers or blocking oligonucleotides, and further
wherein
[0216] e) detecting of the amplificates is carried out by means of
a real-time detection probe, as described above.
[0217] Preferably, where the subsequent amplification of d) is
carried out by means of methylation specific primers, as described
above, said methylation specific primers comprise a sequence having
a length of at least 9 nucleotides which hybridises to a treated
nucleic acid sequence according to one of SEQ ID NOS:170-197,
226-253, 142-169 and 198-225 and sequences complementary thereto,
wherein the base sequence of said oligomers comprise at least one
CpG dinucleotide.
[0218] Step e) of the method, namely the detection of the specific
amplificates indicative of the methylation status of one or more
CpG positions of at least one sequences of the group comprising SEQ
ID NOS:128-141, 114-127 and 100-113 is carried out by means of
real-time detection methods as described above.
[0219] Additional embodiments of the invention provide a method for
the analysis of the methylation status of genomic DNA according to
the invention (e.g., within SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively), and
complements thereof) without the need for bisulfate conversion.
Methods are known in the art wherein a methylation sensitive
restriction enzyme reagent, or a series of restriction enzyme
reagents comprising methylation sensitive restriction enzyme
reagents that distinguishes between methylated and non-methylated
CpG dinucleotides within a target region are utilized in
determining methylation, for example but not limited to DMH.
[0220] In the first step of such additional embodiments, the
genomic DNA sample is isolated from tissue or cellular sources.
Genomic DNA may be isolated by any means standard in the art,
including the use of commercially available kits. Briefly, wherein
the DNA of interest is encapsulated in by a cellular membrane the
biological sample must be disrupted and lysed by enzymatic,
chemical or mechanical means. The DNA solution may then be cleared
of proteins and other contaminants, e.g., by digestion with
proteinase K. The genomic DNA is then recovered from the solution.
This may be carried out by means of a variety of methods including
salting out, organic extraction or binding of the DNA to a solid
phase support. The choice of method will be affected by several
factors including time, expense and required quantity of DNA. All
clinical sample types comprising neoplastic or potentially
neoplastic matter are suitable for use in the present method,
preferred are cell lines, histological slides, biopsies,
paraffin-embedded tissue, body fluids, stool, colonic effluent,
urine, blood plasma, blood serum, whole blood, isolated blood
cells, cells isolated from the blood and combinations thereof. Body
fluids are the preferred source of the DNA; particularly preferred
are blood plasma, blood serum, whole blood, isolated blood cells
and cells isolated from the blood.
[0221] Once the nucleic acids have been extracted, the genomic
double-stranded DNA is used in the analysis.
[0222] In a preferred embodiment, the DNA may be cleaved prior to
treatment with methylation sensitive restriction enzymes. Such
methods are known in the art and may include both physical and
enzymatic means. Particularly preferred is the use of one or a
plurality of restriction enzymes which are not methylation
sensitive, and whose recognition sites are AT rich and do not
comprise CG dinucleotides. The use of such enzymes enables the
conservation of CpG islands and CpG rich regions in the fragmented
DNA. The non-methylation-specific restriction enzymes are
preferably selected from the group consisting of MseI, BfaI, Csp6I,
Tru1I, Tvu1I, Tru9I, Tvu9I, MaeI and XspI. Particularly preferred
is the use of two or three such enzymes. Particularly preferred is
the use of a combination of MseI, BfaI and Csp6I.
[0223] The fragmented DNA may then be ligated to adaptor
oligonucleotides in order to facilitate subsequent enzymatic
amplification. The ligation of oligonucleotides to blunt and sticky
ended DNA fragments is known in the art, and is carried out by
means of dephosphorylation of the ends (e.g., using calf or shrimp
alkaline phosphatase) and subsequent ligation using ligase enzymes
(e.g., T4 DNA ligase) in the presence of dATPs. The adaptor
oligonucleotides are typically at least 18 base pairs in
length.
[0224] In the third step, the DNA (or fragments thereof) is then
digested with one or more methylation sensitive restriction
enzymes. The digestion is carried out such that hydrolysis of the
DNA at the restriction site is informative of the methylation
status of a specific CpG dinucleotide of at least one gene or
genomic sequence selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively).
[0225] Preferably, the methylation-specific restriction enzyme is
selected from the group consisting of Bsi E1, Hga I HinPl, Hpy99I,
Ava I, Bce AI, Bsa HI, BisI, BstUI, BshI236I, AccII, BstFNI, McrBC,
GlaI, MvnI, HpaII (HapII), HhaI, AciI, SmaI, HinP1I, HpyCH4IV, EagI
and mixtures of two or more of the above enzymes. Preferred is a
mixture containing the restriction enzymes BstUI, HpaII, HpyCH4IV
and HinP1I.
[0226] In the fourth step, which is optional but a preferred
embodiment, the restriction fragments are amplified. This is
preferably carried out using a polymerase chain reaction, and said
amplificates may carry suitable detectable labels as discussed
above, namely fluorophore labels, radionuclides and mass labels.
Particularly preferred is amplification by means of an
amplification enzyme and at least two primers comprising, in each
case a contiguous sequence at least 16 nucleotides in length that
is complementary to, or hybridizes under moderately stringent or
stringent conditions to a sequence selected from the group
consisting of SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively), and complements
thereof. Preferably said contiguous sequence is at least 16, 20 or
25 nucleotides in length. In an alternative embodiment said primers
may be complementary to any adaptors linked to the fragments.
[0227] In the fifth step the amplificates are detected. The
detection may be by any means standard in the art, for example, but
not limited to, gel electrophoresis analysis, hybridisation
analysis, incorporation of detectable tags within the PCR products,
DNA array analysis, MALDI or ESI analysis. Preferably said
detection is carried out by hybridisation to at least one nucleic
acid or peptide nucleic acid comprising in each case a contiguous
sequence at least 16 nucleotides in length that is complementary
to, or hybridizes under moderately stringent or stringent
conditions to a sequence selected from the group consisting of SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively), and complements thereof. Preferably
said contiguous sequence is at least 16, 20 or 25 nucleotides in
length.
[0228] Subsequent to the determination of the methylation state or
level of the genomic nucleic acids the presence, absence or class
of CIMP and/or cellular proliferative disorder (e.g., colon cancer)
is deduced based upon the methylation state or level of at least
one CpG dinucleotide sequence of at least one sequence selected
from the group consisting of SEQ ID NOS:128-141, 114-127 and
100-113 (respective genomic, CpG island and amplicons,
respectively), or an average, or a value reflecting an average
methylation state of a plurality of CpG dinucleotide sequences of
at least one sequence selected from the group consisting of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) wherein methylation is associated with
CIMP and/or cellular proliferative disorder (e.g., colon cancer).
Wherein said methylation is determined by quantitative means the
cut-off point for determining said the presence of methylation is
preferably zero (i.e. wherein a sample displays any degree of
methylation it is determined as having a methylated status at the
analysed CpG position). Nonetheless, it is foreseen that the person
skilled in the art may wish to adjust said cut-off value in order
to provide an assay of a particularly preferred sensitivity or
specificity. Accordingly said cut-off value may be increased (thus
increasing the specificity), said cut off value may be within a
range selected form the group consisting of 0%-5%, 5%-10%, 10%-15%,
15%-20%, 20%-30% and 30%-50%. Particularly preferred are the
cut-offs 10%, 15%, 25%, and 30%.
[0229] In an alternative embodiment of the method wherein a panel
of genes comprising BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1,
GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6),
subsequent to the determination of the methylation state of the
genomic nucleic acids the presence, absence or subclass of CIMP
and/or colon proliferative disorders, in particular colorectal cell
proliferative disorder is deduced based upon the methylation state
of at least one CpG dinucleotide sequence of SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively), or an average, or a value reflecting an average
methylation state of a plurality of CpG dinucleotide sequences
thereof wherein hypermethylation is associated with CIMP and/or
colorectal cancer.
Diagnostic and Prognostic Assays for CIMP and/or Cellular
Proliferative Disorders
[0230] The present invention enables diagnosis of events which are
disadvantageous to patients or individuals in which important
genetic and/or epigenetic parameters within at least one gene or
genomic sequence selected from the group consisting of BCL2, BDNF,
CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1,
NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) may be used as markers. Said parameters obtained by
means of the present invention may be compared to another set of
genetic and/or epigenetic parameters, the differences serving as
the basis for a diagnosis and/or prognosis of events which are
disadvantageous to patients or individuals.
[0231] More specifically the present invention enables the
screening of at-risk populations for the early detection of
cancers, most preferably CIMP and/or colorectal carcinomas.
Furthermore, in certain aspects, the present invention enables the
differentiation of neoplastic (e.g. malignant) from benign (i.e.
non-cancerous) cellular proliferative disorders. For example, in
certain embodiments, it enables the differentiation of a colorectal
carcinoma from small colon adenomas or polyps. Neoplastic cellular
proliferative disorders present decreased methylation (i.e.
decreased expression) within at least one gene or genomic sequence
selected from the group consisting of BCL2, BDNF, CACNA1G, CALCA,
CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1
(Table 6); e.g., within SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively), as
opposed to said benign disorders which do not.
[0232] Specifically, the present invention provides for diagnostic
and classification CIMP and/or cancer assays based on measurement
of differential expression (preferably methylation) of one or more
CpG dinucleotide sequences of at least one sequence selected from
the group consisting of SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively) that
comprise such a CpG dinucleotide sequence. Typically, such assays
involve obtaining a sample from a subject, performing an assay to
measure the expression of at least one gene or genomic sequence
selected from the group consisting of BCL2, BDNF, CACNA1G, CALCA,
CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1
(Table 6); e.g., within SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively),
preferably by determining the methylation status of at least one
sequence selected from the group consisting of SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively), derived from the sample, relative to a control
sample, or a known standard and making a diagnosis based
thereon.
[0233] In particular preferred embodiments, inventive oligomers are
used to assess the CpG dinucleotide methylation status, such as
those based on SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively), SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225 or arrays thereof, as
well as in kits based thereon and useful for the diagnosis and/or
classification of cellular proliferative disorders.
Kits
[0234] Moreover, an additional aspect of the present invention is a
kit comprising: a means for determining methylation of at least one
gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively). The means for determining methylation
comprise preferably a bisulfate-containing reagent; one or a
plurality of oligonucleotides consisting whose sequences in each
case are identical, are complementary, or hybridise under stringent
or highly stringent conditions to a 9 or more preferably 18 base
long segment of a sequence selected from SEQ ID NOS:170-197,
226-253, 142-169 and 198-225; and optionally instructions for
carrying out and evaluating the described method of methylation
analysis. In one embodiment the base sequence of said
oligonucleotides comprises at least one CpG, CpA or TpG
dinucleotide.
[0235] In a further embodiment, said kit may further comprise
standard reagents for performing a CpG position-specific
methylation analysis, wherein said analysis comprises one or more
of the following techniques: MS-SNuPE, MSP, MethyLight.TM.,
HeavyMethyl, COBRA, and nucleic acid sequencing. However, a kit
along the lines of the present invention can also contain only part
of the aforementioned components.
[0236] In a preferred embodiment the kit may comprise additional
bisulfite conversion reagents selected from the group consisting:
DNA denaturation buffer; sulfonation buffer; DNA recovery reagents
or kits (e.g., precipitation, ultrafiltration, affinity column);
desulfonation buffer; and DNA recovery components.
[0237] In a further alternative embodiment, the kit may contain,
packaged in separate containers, a polymerase and a reaction buffer
optimised for primer extension mediated by the polymerase, such as
PCR. In another embodiment of the invention the kit further
comprising means for obtaining a biological sample of the patient.
Preferred is a kit, which further comprises a container suitable
for containing the means for determining methylation of at least
one gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively) in the biological sample of the
patient, and most preferably further comprises instructions for use
and interpretation of the kit results. In a preferred embodiment
the kit comprises: (a) a bisulfite reagent; (b) a container
suitable for containing the said bisulfite reagent and the
biological sample of the patient; (c) at least one set of primer
oligonucleotides containing two oligonucleotides whose sequences in
each case are identical, are complementary, or hybridise under
stringent or highly stringent conditions to a 9 or more preferably
18 base long segment of a sequence selected from SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225; and optionally (d)
instructions for use and interpretation of the kit results. In an
alternative preferred embodiment the kit comprises: (a) a bisulfite
reagent; (b) a container suitable for containing the said bisulfite
reagent and the biological sample of the patient; (c) at least one
oligonucleotides and/or PNA-oligomer having a length of at least 9
or 16 nucleotides which is identical to or hybridises to a
pre-treated nucleic acid sequence according to one of SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225 and sequences
complementary thereto; and optionally (d) instructions for use and
interpretation of the kit results.
[0238] In an alternative embodiment the kit comprises: (a) a
bisulfite reagent; (b) a container suitable for containing the said
bisulfite reagent and the biological sample of the patient; (c) at
least one set of primer oligonucleotides containing two
oligonucleotides whose sequences in each case are identical, are
complementary, or hybridise under stringent or highly stringent
conditions to a 9 or more preferably 18 base long segment of a
sequence selected from SEQ ID NOS:170-197, 226-253, 142-169 and
198-225; (d) at least one oligonucleotides and/or PNA-oligomer
having a length of at least 9 or 16 nucleotides which is identical
to or hybridises to a pre-treated nucleic acid sequence according
to one of SEQ ID NOS:170-197, 226-253, 142-169 and 198-225 and
sequences complementary thereto; and optionally (e) instructions
for use and interpretation of the kit results.
[0239] The kit may also contain other components such as buffers or
solutions suitable for blocking, washing or coating, packaged in a
separate container.
[0240] Typical reagents (e.g., as might be found in a typical
COBRA.TM.-based kit) for COBRA.TM. analysis may include, but are
not limited to: PCR primers for at least one gene or genomic
sequence selected from the group consisting of BCL2, BDNF, CACNA1G,
CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1,
RUNX3, SOCS1 (Table 6); e.g., within SEQ ID NOS:128-141, 114-127
and 100-113 (respective genomic, CpG island and amplicons,
respectively); restriction enzyme and appropriate buffer;
gene-hybridization oligo; control hybridization oligo; kinase
labeling kit for oligo probe; and labeled nucleotides. Typical
reagents (e.g., as might be found in a typical MethyLight.TM.-based
kit) for MethyLight.TM. analysis may include, but are not limited
to: PCR primers for the bisulfite converted sequence of at least
one gene or genomic sequence selected from the group consisting of
BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3, HOXA1, IGF2, KL,
NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g., within SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively); bisulfite specific probes (e.g.,
TaqMan.TM. or Lightcycler.TM.); optimized PCR buffers and
deoxynucleotides; and Taq polymerase.
[0241] Typical reagents (e.g., as might be found in a typical
Ms-SNuPE.TM.-based kit) for Ms-SNuPE.TM. analysis may include, but
are not limited to: PCR primers for specific gene (or bisulfite
treated DNA sequence or CpG island); optimized PCR buffers and
deoxynucleotides; gel extraction kit; positive control primers;
Ms-SNuPE.TM. primers for the bisulfite converted sequence of at
least one gene or genomic sequence selected from the group
consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3,
HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g.,
within SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively); reaction buffer (for the
Ms-SNuPE reaction); and labelled nucleotides.
[0242] Typical reagents (e.g., as might be found in a typical
MSP-based kit) for MSP analysis may include, but are not limited
to: methylated and unmethylated PCR primers for the bisulfite
converted sequence of or genomic sequence selected from the group
consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3,
HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g.,
within SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively), optimized PCR buffers and
deoxynucleotides, and specific probes.
[0243] Moreover, an additional aspect of the present invention is
an alternative kit comprising a means for determining methylation
of at least one gene or genomic sequence selected from the group
consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1, DLEC1, GATA3,
HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table 6); e.g.,
within SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively), wherein said means
comprise preferably at least one methylation specific restriction
enzyme; one or a plurality of primer oligonucleotides (preferably
one or a plurality of primer pairs) suitable for the amplification
of a sequence comprising at least one CpG dinucleotide of a
sequence selected from SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively); and
optionally instructions for carrying out and evaluating the
described method of methylation analysis. In one embodiment the
base sequence of said oligonucleotides are identical, are
complementary, or hybridise under stringent or highly stringent
conditions to an at least 18 base long segment of a sequence
selected from SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively).
[0244] In a further embodiment said kit may comprise one or a
plurality of oligonucleotide probes for the analysis of the digest
fragments, preferably said oligonucleotides are identical, are
complementary, or hybridise under stringent or highly stringent
conditions to an at least 16 base long segment of a sequence
selected from SEQ ID NOS:128-141, 114-127 and 100-113 (respective
genomic, CpG island and amplicons, respectively).
[0245] In a preferred embodiment the kit may comprise additional
reagents selected from the group consisting: buffer (e.g.,
restriction enzyme, PCR, storage or washing buffers); DNA recovery
reagents or kits (e.g., precipitation, ultrafiltration, affinity
column) and DNA recovery components.
[0246] In a further alternative embodiment, the kit may contain,
packaged in separate containers, a polymerase and a reaction buffer
optimised for primer extension mediated by the polymerase, such as
PCR. In another embodiment of the invention the kit further
comprising means for obtaining a biological sample of the patient.
In a preferred embodiment the kit comprises: (a) a methylation
sensitive restriction enzyme reagent; (b) a container suitable for
containing the said reagent and the biological sample of the
patient; (c) at least one set of oligonucleotides one or a
plurality of nucleic acids or peptide nucleic acids which are
identical, are complementary, or hybridise under stringent or
highly stringent conditions to an at least 9 base long segment of a
sequence selected from SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively); and
optionally (d) instructions for use and interpretation of the kit
results.
[0247] In an alternative preferred embodiment the kit comprises:
(a) a methylation sensitive restriction enzyme reagent; (b) a
container suitable for containing the said reagent and the
biological sample of the patient; (c) at least one set of primer
oligonucleotides suitable for the amplification of a sequence
comprising at least one CpG dinucleotide of a sequence selected
from SEQ ID NOS:128-141, 114-127 and 100-113 (respective genomic,
CpG island and amplicons, respectively); and optionally (d)
instructions for use and interpretation of the kit results.
[0248] In an alternative embodiment the kit comprises: (a) a
methylation sensitive restriction enzyme reagent; (b) a container
suitable for containing the said reagent and the biological sample
of the patient; (c) at least one set of primer oligonucleotides
suitable for the amplification of a sequence comprising at least
one CpG dinucleotide of a sequence selected from SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively); (d) at least one set of
oligonucleotides one or a plurality of nucleic acids or peptide
nucleic acids which are identical, are complementary, or hybridise
under stringent or highly stringent conditions to an at least 9
base long segment of a sequence selected from SEQ ID NOS:128-141,
114-127 and 100-113 (respective genomic, CpG island and amplicons,
respectively) and optionally (e) instructions for use and
interpretation of the kit results.
[0249] The kit may also contain other components such as buffers or
solutions suitable for blocking, washing or coating, packaged in a
separate container.
[0250] The invention further relates to a kit for use in providing
a diagnosis of the presence of a cell proliferative disorder in a
subject by means of methylation-sensitive restriction enzyme
analysis. Said kit comprises a container and a DNA microarray
component. Said DNA microarray component being a surface upon which
a plurality of oligonucleotides are immobilized at designated
positions and wherein the oligonucleotide comprises at least one
CpG methylation site. At least one of said oligonucleotides is
specific for the at least one gene or genomic sequence selected
from the group consisting of BCL2, BDNF, CACNA1G, CALCA, CRABP1,
DLEC1, GATA3, HOXA1, IGF2, KL, NEUROG1, NR3C1, RUNX3, SOCS1 (Table
6); e.g., within SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively) and
comprises a sequence of at least 15 base pairs in length but no
more than 200 bp of a sequence according to one of SEQ ID
NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively). Preferably said sequence is at least
15 base pairs in length but no more than 80 bp of a sequence
according to one of SEQ ID NOS:128-141, 114-127 and 100-113
(respective genomic, CpG island and amplicons, respectively). It is
further preferred that said sequence is at least 20 base pairs in
length but no more than 30 bp of a sequence according to one of SEQ
ID NOS:128-141, 114-127 and 100-113 (respective genomic, CpG island
and amplicons, respectively).
[0251] Said test kit preferably further comprises a restriction
enzyme component comprising one or a plurality of
methylation-sensitive restriction enzymes.
[0252] In a further embodiment said test kit is further
characterized in that it comprises at least one
methylation-specific restriction enzyme, and wherein the
oligonucleotides comprise a restriction site of said at least one
methylation specific restriction enzymes.
[0253] The kit may further comprise one or several of the following
components, which are known in the art for DNA enrichment: a
protein component, said protein binding selectively to methylated
DNA; a triplex-forming nucleic acid component, one or a plurality
of linkers, optionally in a suitable solution; substances or
solutions for performing a ligation e.g. ligases, buffers;
substances or solutions for performing a column chromatography;
substances or solutions for performing an immunology based
enrichment (e.g. immunoprecipitation); substances or solutions for
performing a nucleic acid amplification e.g. PCR; a dye or several
dyes, if applicable with a coupling reagent, if applicable in a
solution; substances or solutions for performing a hybridization;
and/or substances or solutions for performing a washing step.
[0254] The described invention further provides a composition of
matter useful for detecting, differentiation and distinguishing
between colon cell proliferative disorders. Said composition
comprising at least one nucleic acid 18 base pairs in length of a
segment of the nucleic acid sequence disclosed in SEQ ID
NOS:170-197, 226-253, 142-169 and 198-225, and one or more
substances taken from the group comprising: 1-5 mM Magnesium
Chloride, 100-500 .mu.M dNTP, 0.5-5 units of taq polymerase, bovine
serum albumen, an oligomer in particular an oligonucleotide or
peptide nucleic acid (PNA)-oligomer, said oligomer comprising in
each case at least one base sequence having a length of at least 9
nucleotides which is complementary to, or hybridizes under
moderately stringent or stringent conditions to a pretreated
genomic DNA according to one of the SEQ ID NOS:170-197, 226-253,
142-169 and 198-225 and sequences complementary thereto. It is
preferred that said composition of matter comprises a buffer
solution appropriate for the stabilization of said nucleic acid in
an aqueous solution and enabling polymerase based reactions within
said solution. Suitable buffers are known in the art and
commercially available.
[0255] In further preferred embodiments of the invention said at
least one nucleic acid is at least 50, 100, 150, 200, 250 or 500
base pairs in length of a segment of the nucleic acid sequence
disclosed in SEQ ID NOS:170-197, 226-253, 142-169 and 198-225.
[0256] While the present invention has been described with
specificity in accordance with certain of its preferred
embodiments, the following examples serve only to illustrate the
invention and are not intended to limit the invention within the
principles and scope of the broadest interpretations and equivalent
configurations thereof.
Example 1
Rationale, Samples and Methods for Studies of CpG Island Methylator
Phenotype in Human Colorectal Cancer
[0257] Rationale. As reviewed above, a lack of uniform standards
and systematic marker development has created confusion and
uncertainty in the field regarding the precise classification of
the CpG Island Methylator Phenotype (CIMP).
[0258] Unsupervised two-dimensional cluster analysis of DNA
methylation data for large numbers of markers and large numbers of
tumor samples can be a useful tool to identify such distinct
correlated subsets of tumors and markers. Although microarray-based
methods for screening CpG island hypermethylation have been
developed, most are not limited to Type C loci, and most are not
readily applied to large numbers of tumor samples, which is
required to obtain stable clusters.
[0259] Therefore, to resolve the controversy surrounding the
validity of a distinct CpG island methylator phenotype, we explored
the DNA methylation behavior of human colorectal tumors using
automated real-time PCR-based MethyLight.TM., which is capable of
rapidly assessing the methylation status of more than 100 different
CpG islands on more than 100 different tissue samples.
Specifically, an initial systematic evaluation of DNA methylation
markers was conducted by the present applicants to address the
following three issues:
[0260] First, and most importantly, a confirmation that CIMP could
be recognized as a distinct subgroup of colorectal cancer was
needed. Although the CIMP trait is widely accepted in the
scientific community, and has been reported as a distinct entity by
a very large number of independent laboratories, a recently
published influential report suggests that CpG island
hypermethylation frequency is a continuous trait in human
colorectal cancer, without a clearly defined separate subgroup of
tumors with frequent CpG island hypermethylation (38). It was thus
of interest to confirm whether this report was due to the inclusion
of a large number of CpG islands that do not belong to the CIMP
subset, or whether there is indeed little justification for
separating out a distinct subset of colorectal cancers. To avoid
bias either for or against the existence of CIMP, applicants
started a multi-step screen with 195 unique MethyLight.TM. CpG
island hypermethylation assays available in applicants' laboratory,
and used both previously characterized CIMP+ and CIMP- tumors to
select cancer-specific methylation markers for further evaluation
(see below). Furthermore, applicants used a battery of cluster
analysis routines to evaluate whether CIMP tumors form a distinct
grouping, and used several different gene selection algorithms,
including gene filter, logistic regression, classification and
regression trees (CART) (140) and gene shaving (141) to choose
potential CIMP markers for further consideration (see below).
[0261] Second, a CIMP analysis technology platform was needed that
could accommodate the difficult requirements of a large-scale
study, including i) compatibility with very limited amounts of
formalin-fixed, paraffin-embedded tissue (a single 5-.mu.m
microscopic slide), ii) robust performance, regardless of DNA
quantity and quality (traditional gel-based methylation-specific
PCR (MSP) is DNA quality and quantity-dependent), and iii)
automatable analysis, to accommodate large numbers of specimens,
while minimizing human error, cross contamination, and post-PCR
contamination (see below).
[0262] Third, if CIMP could be verified, then the question as to
whether the existing classification panels of markers were
sufficient, or whether better panels could be developed needed to
be evaluated. Applicants perceived in advance that the evaluation
of classification panels would be particularly problematic without
an external gold standard for CIMP classification. Therefore, a
cross-panel misclassfication error rate strategy was selected by
applicants to best confront this challenge (see below).
[0263] These issues needed to be resolved to enable the
characterization of CIMP in a large-scale population-based
studies.
Materials and Methods
[0264] Colorectal Cancer Specimens.
[0265] DNA samples were drawn from the Walter Paulson Tumour Bank,
a consecutive series of over 800 colorectal cancer cases collected
at the Royal Brisbane and Womens Hospital between 1989 and 2004.
Patients gave informed consent in writing to the use of their bowel
tissue for research. The study was approved by the Royal Brisbane
Hospital Human Research Ethics Committee, the Bancroft Centre
Ethics Committee and the USC Institutional Review Board. Tumors
were collected at surgery and representative sections for research
were removed by a pathologist. DNA was extracted using a
modification of the salt precipitation technique of Miller et
al..sup.13. The first screen (FIG. 1) included five CIMP+ tumors,
and five CIMP- tumors, which had been previously assessed for their
CIMP status using a panel of four MINT loci (1, 2, 12 and 31) and
MLH1, analyzed by combined bisulfate restriction analysis (COBRA)
and three Type C genes (p14/ARF, CDKN2A (p16), MGMT), analyzed by
gel-based methylation specific PCR (MSP).sup.14. CIMP+ tumors were
defined as having 80-100% of the markers methylated, while the five
CIMP- tumors had an average of only 11% of markers methylated.
Other tumor characteristics were obtained by standard techniques.
KRAS (K-ras) mutation analysis at codons 12 and 13 was performed
using direct automated sequencing of a fragment containing codon 12
and 13 in exon 1 of the KRAS gene, amplified using a touchdown PCR
cycle and hotstart protocol. BRAF (B-raf) mutation analysis at
codon 600 (V600E; formerly V599E.sup.15) was performed by a
real-time PCR-based allelic discrimination method, as
described.sup.16. Microsatellite instability was determined as
described.sup.17. Eight cases of MSI-H cancer showed features
consistent with HNPCC including young age of onset (average 44,
range 31-53), family history, lack of MSH2 expression by tumor
immunohistochemistry (n=4), and germline mutation of a mismatch
repair gene (n=4).
[0266] DNA Methylation Analysis Technology.
[0267] Treatment of genomic DNA with sodium bisulfite, followed by
alkaline treatment converts unmethylated cytosines to uracil, while
leaving methylated cytosine residues intact was used. Sequence
variants at a particular locus can subsequently be analyzed by PCR
amplification with primers designed to anneal with
bisulfite-converted DNA. The benefit of sodium-bisulfite-based
assays is that they require very small amounts of DNA and
consequently, are compatible with DNA obtained from microdissected
paraffin-embedded tissue samples (8, 145-158). However, until the
development of the MethyLight.TM. assay (6-8, 20, 106),
bisulfite-based DNA methylation detection required gel
electrophoresis and many of the techniques also employed
restriction enzyme digestion, radiolabeled dNTPs, or hybridization
probes. These labor-intensive steps limited the use of these
methods for high-throughput analyses. More recently,
sodium-bisulfite-based microarray platforms have been developed.
However, these platforms still require separate PCR amplification
of each target locus to hybridize with the chip. With
MethyLight.TM. technology, the methylation analysis is complete, as
soon as the PCR reaction is finished. With microarray-based
detection, the PCR amplificates need to be subsequently hybridized
to the microarray, the hybridization signal needs to be captured,
and then interpreted to yield DNA methylation measurements.
Therefore, compared to MethyLight.TM., the current chip-based
platforms are neither more cost-effective, nor less
labor-intensive. More importantly, however, MethyLight.TM. is much
better suited for the analysis of challenging samples, such as
formalin-fixed paraffin-embedded samples with small amounts of
highly degraded cross-linked DNA. The average amplicon size for
reactions developed in applicants' laboratory is about 80 bp, which
is well below the median amplifiable fragment size from
formalin-fixed tissues. The MethyLight.TM. technique has been
cross-validated with several other DNA methylation analysis
techniques, including COBRA and bisulfite genomic sequencing (6).
MethyLight.TM. is rapidly becoming the method of choice for
large-scale automated DNA methylation studies requiring high
sensitivity (8, 20, 98, 99, 106, 161-174), and has been adopted by
many other laboratories.
[0268] Therefore, genomic DNA was treated with sodium bisulfite and
subsequently analyzed by MethyLight.TM. as described.sup.18,19. A
complete list of all MethyLight.TM. reactions is provided in TABLE
1 (supplemental table 1). MethyLight.TM. data are reported as a
ratio between the value derived from the real-time PCR standard
curve plotted as log (quantity) versus threshold C(t) value for the
methylation reaction and likewise for a methylation-independent
control reaction. However, since such a ratio is dimensionless, can
vary from gene to gene, and is affected by many experimental
parameters, such as primer and probe batches, it is useful to
normalize this ratio to the ratio obtained for a constant reference
sample. M.SssI-treated genomic DNA, frozen in aliquots, was used as
a constant reference sample to determine this ratio and to derive
the standard curve.sup.20. Thus, the Percent of Methylated
Reference ("PMR") can be defined as 100*(METHYLATED
REACTION/CONTROL REACTION).sub.sample/(METHYLATED REACTION/CONTROL
REACTION).sub.M.SssI-Reference, in which "METHYLATED REACTION"
refers to the methylation measurement at a particular locus and
"CONTROL REACTION" refers to the methylation-independent
measurement using the control reaction.sup.20. Applicants have
developed an improved normalization control reaction based on
dispersed Alu repeats.sup.18. For the data supplied in FIG. 1,
COL2A1 was used as a normalization control reaction.sup.21. For the
data in FIG. 2, the mean of PMR values derived with COL2A1 and ALU
was used. For the data in FIGS. 3, 4 and 5, PMR values were derived
exclusively using the ALU normalization control reaction. This ALU
normalization reaction is methylation independent.sup.18, and not
the methylation-dependent ALU reactions, which we have also
previously described.sup.18. Applicants currently rely on the ALU
normalization reaction, since it is less prone to fluctuations
caused by aneuploidy and copy number changes affecting single-copy
normalization reactions.
[0269] Selection of Type C Markers.
[0270] Applicants screened all 195 available MethyLight.TM. markers
in the Laird laboratory to identify Type C markers in colorectal
cancer. Most of these markers had been developed for other
purposes, including studies of esophageal cancer, lung cancer,
pancreatic cancer, ovarian cancer, brain cancer and
neurodegenerative disorders. Therefore, this starting collection is
not likely to be biased with respect to CIMP analysis. Applicants
used five CIMP+ tumors, and five CIMP- tumors, which had been
previously assessed for their CIMP status as described above.
Applicants used three criteria to select Type C markers for further
evaluation. First, any marker for which the highest PMR value among
these 20 samples was less than 2 was excluded. Second, any marker
for which the mean PMR for tumor samples was at least twice that of
normal samples was included. Third, any marker for which all normal
samples had a PMR<2 and for which all tumor samples had a
PMR>2 was included. Both the second and third criteria were
applied separately to both the CIMP+ tumors and CIMP- tumors as
well as to all ten cases collectively. A marker was included if it
passed at least one of the criteria in any of the three sample
comparisons (CIMP-, CIMP+, or both). Applicants applied this
relatively relaxed, inclusive marker filter primarily to eliminate
non-cancer-specifically methylated markers, not to identify top
marker candidates. This marker screen resulted in a collection of
92 reactions that passed this first screen for tumor-specificity.
Among these 92 reactions were five methylation markers (CDKN2A
(p16), MLH1, MINT1, MINT2, and MINT31) that have been commonly used
to define CIMP status.
[0271] Cluster Analysis.
[0272] For the hierarchical cluster analysis shown in FIG. 2,
applicants grouped the PMR values for each marker into quartiles.
Applicants used Manhattan distance and average linkage to perform
the clustering.sup.7. Two distinct clusters were identified.
Applicants also performed three other clustering routines (not
shown). For the second clustering routine, applicants defined loci
with PMR values above 10 as methylated and counted the number of
methylated loci for each tumor. The resulting index was clustered
using PAM (partitioning around medioids).sup.7. The number of
clusters was selected by choosing the number that gave the highest
average silhouette width. This method selected two clusters. The
third method, the Gaussian mixture model.sup.8, was fit using the
top nine principal components of the log-transformed data
(ln(PMR+1)). These nine principal components explained 78% of the
variability in the DNA methylation data. Using the BIC curve to
select the number of clusters, applicants selected four. The final
method, PAM, was also fit to the log-transformed data. Again two
clusters were suggested by the average silhouette width. Using
these four different algorithms applicants saw remarkable overlap
in subjects that fell into what applicants call our CIMP+ cluster.
A subset of six individuals was defined as CIMP+ by all four
clustering methods. Another seven individuals are identified as
CIMP+ by at least one method. The remaining 35 subjects were
classified as CIMP- by all methods. Scaling the data before
conducting the principal components analysis, or before clustering
with PAM, had little effect on the results.
[0273] CIMP Marker Selection.
[0274] Applicants used the different cluster routines described
above to classify tumors as either CIMP+ or CIMP-. This yielded
similar but distinct classifications of CIMP+ or CIMP-. Applicants
then applied three different marker selection algorithms (gene
filter, classification and regression trees (CART).sup.22, logistic
regression) to these classifications to identify those markers that
best identify CIMP. For each marker selection algorithm, applicants
ranked the importance of each marker for each definition of CIMP
and selected the five markers for each algorithm that ranked the
highest across all definitions of CIMP. Applicants also applied a
fourth marker selection algorithm (gene shaving.sup.23) designed to
select the markers that explain the most variability in the data
without knowledge of CIMP status.
[0275] For the gene filter approach, applicants ranked the
individual markers based on their ability to predict CIMP+ using
the Wilcoxon test. Multivariable models to predict CIMP+ were
created using CART and logistic regression. For logistic
regression, forward stepwise regression to was used to select the
five most significant markers for each outcome. CART selected at
most two markers before the CIMP+ group was perfectly identified
for each of the four gene cluster algorithms. Each of the first
three approaches was fit to each of the definitions of CIMP+. The
markers were ranked in terms of importance and those achieving the
highest rank across all CIMP definitions were selected. For gene
shaving, we shaved off 10% of the markers at each step. A total of
36 markers (39%) are identified as explaining the most variability
in the tumor samples. From this subset, applicants selected the
five markers that explained the most variability in the
samples.
[0276] The four CIMP marker selection algorithms described above
each generated five top CIMP marker candidates. The union of
markers from the overlapping sets comprised a panel of 9
CIMP-predicting markers. Although the five most popular traditional
CIMP markers (CDKN2A (p16), MINT1, MINT2, MINT31, and MLH1) all
participated in the 92-marker cluster routines and marker selection
strategies, none of these five markers was selected as one of the
top five candidates in any of the marker selection algorithms.
[0277] As an added precaution, in addition to the nine
CIMP-specific markers selected above, applicants also included the
five best Type-C markers among the CIMP-specific markers, selected
using a gene filter approach, since the CIMP marker selection
strategies described above were driven by a relatively small number
of CIMP+ tumors. Inappropriately retained markers would be expected
to drop out at subsequent screening steps. Using a PMR of 10 to
define positive methylation, applicants found the subset of markers
that had no methylation in adjacent normal tissue (N=31 markers,
all PMR values<10). In this subset, applicants ranked markers by
the number of samples that showed positive methylation (PMR>10)
in tumor tissue. Fourteen markers show more frequent methylation in
tumor tissue than in normal tissue (all p<0.008). Five of the
fourteen markers are markers that have been selected as
CIMP-predicting (CRABP1, NR3C1, BCL2, BDNF, CACNA1G). The remaining
nine are correlated with the selected markers. Applicants selected
the top five Type C markers that were not already selected in a
previous panel.
[0278] New CIMP Classification Panel.
[0279] Applicants dichotomized all PMR values at 10 to simplify
panel assembly and to allow for an easier translation of our panels
to gel-based MSP.sup.24, which outputs only nominal data
(methylated or unmethylated) (FIG. 6). The dichotomization
threshold of PMR=10 was chosen as a point sufficiently above
background measurements of methylation, using the MethyLight.TM.
technique, possibly reflecting low rates of stochastic
hypermethylation, yet well below the much higher PMR values
generally obtained for hypermethylation observed for most markers
in colorectal tumors. This definition yields 85% specificity in
normal mucosa for the CIMP-specific markers identified in the
clustering analysis routines. Applicants strove to identify as
small a panel as possible that would accurately identify CIMP+
tumors, since this would provide the most cost-effective screening
method for CIMP status. Based on the results obtained in FIG. 4,
applicants considered a panel of five markers to be sufficient to
detect bimodal methylation distribution among tumors, and to thus
provide reasonably accurate CIMP classification ability. Applicants
subsequently also tested larger panels of ten markers but found
very little gain in classification accuracy. To select the best
possible five-marker panel from the 14 markers shown in FIG. 3,
applicants evaluated all 2,002 possible five-marker panels by
measuring a panel's ability to identify CIMP by its bimodal
appearance. Statistically, bimodal appearance is measured by the
proportion of total variability of our index (0-5 methylated loci)
explained by our classification rule (3-5 methylated loci=CIMP+,
0-2 methylated loci=CIMP-). The larger the variance explained, the
more bimodal the distribution. The threshold of three or more
methylated loci for defining CIMP+ tumors was established by
minimizing the within group sum of squared errors. Applicants also
considered MethyLight.TM. reaction performance characteristics of
each individual marker. Among the most important reaction
characteristics is the real-time PCR threshold cycle (C(t) value)
on a standardized sample of fully methylated DNA, which is a
measure of reaction sensitivity and efficiency. This measure is of
particular importance for the analysis of samples containing
borderline quantities of DNA. Applicants also considered the delta
C(t) value for methylated, versus unmethylated human genomic
reference DNA samples--a measure of methylation specificity for the
reaction. As a final criterion, applicants also considered a
bimodal distribution of the PMR values for the individual
markers--markers with a large fraction of intermediate methylation
measurements will more likely cause misclassification than markers
that are more bimodal in their distribution of PMR values. One of
the criteria that was specifically not considered is the functional
consequence of the CpG island hypermethylation event. Applicants
hypothesize that CIMP represents an epigenetic control defect and
that many of the markers that may best reflect this defect may be
of no functional significance to tumorigenesis. The five-marker
panel that best satisfied all of the criteria described above and
retained a high ranking in their ability to explain the percent of
variance by the CIMP definition consisted of CACNA1G, IGF2,
NEUROG1, RUNX3, and SOCS1 (TABLE 2 (supplemental table 2)).
TABLE-US-00003 TABLE 1 (supplementary table 1); Complete list of
all MethyLight .TM. reactions. HUGO 14 CIMP Gene and 5 Name
Reaction Cancer Toyota (If Reaction Design Specific? Markers?
Available) ID Code Aliases (Y/N) (Y/N) ABCB1 ABCB1- HB- MDR1; Y N
M1B 051 PGY1/ATP- binding cassette, sub-family B (MDR/TAP), member
1 APC APC- HB- Adenomatous Y N M1B 153 polyposis coli APEX1 APEX1-
HB- APEX N N M1B 090 nuclease (multi- functional DNA repair enzyme
1) APP APP- HB- Amyloid N N M1B 266 beta (A4) precursor protein
(protease nexin-II, Alzheimer disease) ARF/ ARF- HB- p14 ARF; Y N
CDKN2A M1B 196 alternate reading frame of CDKN2A ARP ARPC1B- HB-
Actin N N C1B M1B 186 related protein 2/3 complex, subunit 1B, 41
kDa; ARC41 ATM ATM- HB- Ataxia N N M1B 179 telangiect asia mutated
(includes comple- mentation groups A, ATR ATR- HB- Ataxia N N M1B
180 telangiect asia and Rad3 related; FRP1; SCKL; SCKL1 AXIN1
AXIN1- HB- Axin 1 N N M1B 227 BCL2 BCL2- HB- Bcl-2; B- Y Y M1B 140
cell CLL/ lymphoma2 BDNF BDNF- HB- Brain Y Y M2B 258 derived
neurotrpohic factor BRCA1 BRCA1- HB- Breast N N M1B 045 cancer 1,
early onset; RNF53; BRCC1 BRCA2 BRCA2- HB- breast N N M1B 126
cancer 2, early onset CACNA1G CACNA1G- HB- Calcium Y Y M1B 158
channel, voltage- dependent, alpha 1G subunit CALCA CALCA- HB-
Calcitonin/ Y Y M1B 166 calcitonin- related polypeptide, alpha;
CALC1 CCND1 CCND1- HB Cyclin D1 N N M1B - (PRAD1: parathyroid
adenomatosis 1); BCL1; D11S287E CCND2 CCND2- HB- Cyclin D2 Y N M1B
040 CDH1 CDH1- HB- E- N N M2B 050 cadherin (epithelial)/ Cadherin
1, type 1 CDH13 CDH13- HB- H- Y N M1B 075 Cadherin/ Cadherin 13, H-
cadherin (heart)/T- cadherin; CDHH CDK2AP1 CDK2AP1- HB- CDK2- N N
M1B 226 associated 1 protein 1; DOC-1 (Deleted in oral cancer);
DORC1; ST19 CDKN1A CDKN1A- HB- Cyclin- N N M1B 230 dependent kinase
inhibitor 1A; p21; Cip1p21; CIP1; SDI1; WAF1; CAP20; CDKN1 CDKN1C
CDKN1C- HB- Cyclin- Y N M2B 329 dependent kinase inhibitor 1C (p57,
Kip2)/BWS; WBS; BWCR; KIP2 CDKN2A CDKN2A- HB- p16/Cyclin- Y Y M2B
081 dependent kinase inhibitor 2A; CDKN2; CDK4I; p16; INK4a; MTS1;
CMM2 CDKN2B CDKN2B- HB- p15/Cyclin- N N M1B 173 dependent kinase t
inhibitor 2B; P15; MTS2; INK4B CDX1 CDX1- HB- Caudal N N M1B 195
type homeo box transcription factor CGA CGA- HB- Glycoprotein N N
M1B 237 hormones, alpha polypeptide; GPHa; GPHA1 CHFR CHFR- HB-
Checkpoint Y N M1B 190 with forkhead and ring finger domains;
FLJ10796 CLDN1 CLDN1- HB- Claudin-1 N N M1B 059 CLIC4 CLIC4- HB-
Chloride N N M1B 062 intracellular channel 4 COL1A2 COL1A2- HB-
Collagen, Y N M1B 193 type 1, alpha 2; OI4 CRABP1 CRABP1- HB-
Cellular Y Y M1B 197 retinoic acid binding protein 1; RBP5; CRABP;
CRABPI; CRABP-I CTNNB1 CTNNB1- HB- Catenin N N M1B 170 (cadherin-
associated protein), beta 1, 88 kDa; CTNNB CTSD CTSD- HB- Cathepsin
N N M1B 147 D (lysosomal aspartyl protease); CPSD CXADR CXADR- HB-
Coxsackie N N
M1B 054 virus and adenovirus receptor; CAR CYP1B1 CYP1B1- HB-
Cytochrome Y N M1B 078 P450, family 1, subfamily B, polypeptide 1;
GLC3A; CP1B CYP27B1 CYB27B1- HB- Cytochrome Y N M1B 223 P450,
family 27, subfamily B, polypeptide 1; CYP1; VDD1; PDDR DAPK1
DAPK1- HB- Death- N N M1B 046 associated protein kinase 1 DCC DCC-
HB- Deleted in Y N M1B 178 colorectal carcinomas DCLRE1C DCLRE1C-
HB- ARTEMIS/ N N M1B 133 hypothetical protein FLJ11360; artemis
protein; DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae);
SNM1C; A-SCID DDB1 DDB1- HB- Damage- N N M1B 116 specific DNA
binding protein 1, 127 kDa DIRAS3 DIRAS3- HB- Ras N N M1B 043
homolog gene family, member I/NOEY2; DIRAS family, GTP- binding
RAS-like 3; ARHI DLC1 DLC1- HB- Deleted in Y N M1B 218 liver cancer
1; HP; ARHGAP 7; STARD12; FLJ21120; DLC-1; p122- RhoGAP DLEC1 DLEC-
HB- Deleted in Y Y M1B 225 lung and esophageal cancer 1 DNAJC1
DNAJC15- HB- DnaJ N N M1B 048 (Hsp40) homolog, subfamily C, member
15; DNAJD1; MCJ DPH1 DPH1- HB- Dipthamide N N M1B 049 Biosynthesis
Protein 2, S. cerevisiae, Homolog- Like 1; Candidate tumor
suppressor in ovarian cancer 2 (OVCA2); DHP2L1; DPH2L DRD1 DRD1-
HB- Dopamine Y N M1B 252 receptor D1 DRD2 DRD2- HB- Dopamine Y N
M1B 253 receptor D2 EBF3 EBF3- HB- COE3; Y N M1B 229 Early B- cell
factor 3; DKFZp66 7B0210 EPM2AIP EPM2AIP- HB- EPM2A Y N M1B 152
(laforin) interacting protein 1; KIAA0766; FLJ11207 ERBB2 ERBB2-
HB- Epidermal N N M2B 233 growth factor receptor (erythro- blastic
leukemia viral (v- erb-b) oncogene homolog, avian); NGL; HER-2; NEU
ERCC1 ERCC1- HB- Excision Y N M1B 110 repair cross- complementing
rodent repair deficiency comple- mentation group 1 (includes
overlapping antisense sequence) ERCC2 ERCC2- HB- Excision N N M1B
105 repair cross- comple- menting rodent repair deficiency, comple-
mentation group 2 (xeroderma pigmentosum D; XPD) ERCC4 ERCC4- HB-
Excision N N M1B 111 repair cross- comple- menting rodent repair
deficiency, comple- mentation group 4; RAD1; XPF ERCC5 ERCC5- HB-
Excision N N M1B 109 repair cross- comple- menting rodent repair
deficiency, comple- mentation group 5 (xeroderma pigmentosum,
comple- mentation group G (Cockayne syndrome)); ERCM2; XPGC ERCC6
ERCC6- HB- Excision N N M1B 114 repair cross- comple- menting
rodent repair deficiency, comple- mentation group 6 (PGBD3); CKN2;
CSB; RAD26 ERCC8 ERCC8 HB- Excision N N M1B 113 repair cross-
comple- menting rodent repair deficiency, comple- mentation group 8
(ERCC8); Cockayne
syndrome 1 (classical), CKN1; CSA ESR1 ESR1- HB- Estrogen Y N M1B
164 Receptor Alpha; NR3A1; Era ESR2 ESR2- HB- Estrogen Y N M1B 165
receptor 2 (ER beta); NR3A2; Erb FAF1 FAF1- HB- Fas N N M1B 304
(TNFRSF 6) associated factor 1; CGI-03; hFAF1 FBXW7 FBXW7- HB-
F-box and N N M1B 15 WD-40 1 domain protein 7 (archipelago homolog,
Drosophila); AGO; FLJ11071; SEL-10; SEL10; FBW7; CDC4; FBXW6 FHIT
FHIT- HB- Fragile Y N M2B 041 Histidine Triad; FRA3B; AP3Aase
GABRA2 GABRA2- HB- Gamma- Y N M1B 254 aminobutyric acid A receptor,
alpha 2 GAD1 GAD1- HB- Glutamine Y N M2B 256 decarboxylase 1 (67
kda) GATA3 GATA3- HB- GATA Y Y M1B 327 binding protein 3/HDR;
MGC5445 GATA4 GATA4- HB-323 GATA Y N M1B binding protein 4 GATA5
GATA5- HB- GATA Y N M1B 326 binding protein 5; bB379O24.1 GDNF
GDNF- HB- Glial cell Y N M1B 221 derived neurotrophic factor GRIN2B
GRIN2B- HB- Glutamate Y N M1B 250 receptor, ionotrophic, N-
methyl-D- aspartate 2B (NR3); NMDAR2B GSTP1 GSTP1- HB- Glutathione-
Y N M1B 172 S transferase pi1; FAEES; GST3 HIC1 HIC1- HB-
Hypermethy- Y N M1B 168 lated in cancer 1; ZBTB29 HLA-G HLA-G- HB-
HLA-G N N M1B 215 histocompat- ibility antigen, class I, G HOXA1
HOXA1- HB- Homeo Y Y M2B 268 box A1; HOX1F HOXA10 HOXA10- HB- Homeo
N N M1B 270 box A10; HOX1H HOXA11 HOXA11- HB- Homeo N N M1B 272 box
A11; HOX1I HRAS HRAS- HB- V-Ha-ras N N M1B 144 Harvey rat sarcoma
viral oncogene homolog; HRAS1 HSD17B4 HSD17B4- HB- 17beta- N N M1B
066 hydroxy- 06 steroid dehydroge- nase IV ICAM1 ICAM1B- HB-
Intercellular N N M1B 076 adhesion molecule 1 (CD54), human
rhinovirus receptor; BB2; CD54 IFNG IFNG- HB- Interferon N N M1M
311 Gamma IGF2 IGF2- HB- IGF2: Y Y M2B 319 Insulin- like growth
factor (somatomedin A) IGSF4 IGSF4- HB- Immuno- Y N M1B 069
globulin superfamily, member 4; TSLC1; NECL2; ST17; BL2; SYNCAM;
IGSF4A ITGA4 ITGA4- HB- integrin, Y N M1B 321 alpha 4 (antigen
CD49D, alpha 4 subunit of VLA-4 receptor); CD49D JUP JUP- HB-
Junction Y N M1B 203 Plakoglobin; CTNNG; PDGB; PKGB; DPIII KL KL-
HB- human Y Y M1B 175 Klotho gene LDLR LDLR- HB- Low N N M1B 219
density lipoprotein receptor (familial hypercho- lestero- lemia)
LIG3 LIG3- HB- ligase III, N N M1B 091 DNA, ATP- dependent LPHN2
LPHN2- HB- Latrophilin Y N M1B 202 2; LEC1; LPHH1; KIAA0786 LZTS1
LZTS1- HB- Leucine N N M1B 200 zipper, putative tumor suppressor 1
F37; FEZ1 MBD2 MBD2- HB- Methyl- N N M1B 142 CpG binding domain
protein 2 MBD4 MBD4- HB- Methyl- N N M1B 083 CpG binding domain
protein 4 MGMT MGMT- HB- O-6- Y N M2B 160 methyl- guanine- DNA
methyl- transferase MINT1 MINT1- HB- Colon Y Y M1B 161 cancer
differen- tially methylated CpG island genomic sequence; PROTEIN 2C
(SV2C) in rats MINT2 MINT2- HB- Colon Y Y M1B 187 cancer differen-
tially
methylated CpG island genomic sequence MINT31 MINT31- HB- Colon Y Y
M1B 162 cancer differen- tially methylated CpG island genomic
sequence MLH1 MLH1- HB- Mut L Y Y M2B 150 Homolog 1 (E. coli)
(colon cancer, nonpolyposis type 2); COCA2 MLH3 MLH3- HB- MutL (E.
Y N M1B 099 coli) homolog 3 MMS19L MMS19L- HB- MMS19 N N M1B 117
(MET18 S. cerevisiae)- like; MET-18, hMMS19 MSH2 MSH2- HB- MutS (E.
N N M1B 095 coli) homolog (colon cancer, nonpolyposis type 1);
COCA1 MSH4 MSH4- HB- MutS (E. N N M1B 096 coli) homolog 4 MSH5
MSH5- HB- MutS (E. N N M1B 097 coli) homolog 5 MSH6 MSH6- HB- MutS
(E. Y N M1B 084 coli) homolog 6; GTBP MT1A MT1A- HB- Metallo- Y N
M1B 205 thionein 1A; MT1S; K01383 MT1T MT1G- HB- Metallo- Y N M1B
204 thionein 1G MT2A MT2A- HB- Metallo- Y N M1B 206 thionein 2A;
Metallo- thionein-II; MT2 MT3 MT3- HB- Metallo- Y N M1B 207
thionein 3 (growth inhibitory factor (neuro- trophic)) MTHFR MTHFR-
HB- 5,10- N N M1B 058 methyl- enetetra- hydrofolate reductase
(NADPH) MUTYH MUTYH- HB- MutY (E. N N M1B 088 coli) homolog; MYH
MYOD1 MYOD1- HB- Myogenic Y N M1B 154 determining factor 3; MYF3
NCL NCL- HB- Nucleolin N N M1B 077 NEUROD1 NEUROD1- HB- Neurogenic
Y N M1B 259 differenti- ation 1; NeuroD; BETA2. BHF-1 NEUROD2
NEUROD2- HB- Neurogenic Y N M1B 260 differenti- ation 2; NDRF
NEUROG1 NEUROG1- HB- Neurogenin Y Y M1B 261 1; NEUROD 3; AKA NR3C1
NR3C1- HB- Glucocorti- Y Y M1B 067 coid Receptor/ Nuclear receptor
subfamily 3, group C, member 1; GRL; GR NTF3 NTF3- HB- Neurotrophin
N N M1B 251 3 NTHL1 NTHL1- HB- Nth N N M1B 089 (E. coli
endonuclease III)- like 1; NTH1; OCTS3 OGG1 OGG1- HB 8- N N M1B 087
oxoguanine DNA glycosylase ONECUT2 ONECUT2- HB- One cut N N M1B 242
domain, family member 2. OC-2 OPCML.sup.b OPCML- HB- Opioid Y N M1B
209 binding protein/cell adhesion molecule- like; OPCM, OBCAM
PARP1.sup.c PARP1- HB- Poly N N M1B 093 (ADP- ribose) polymerase
family, member 1; PPOL; ADPRT; PARP PARP2 PARP2- HB- Poly(ADP- N N
M1B 094 ribose) polymerase) family, member 2; ADPRTL2 PAX8 PAX8-
HB- Paired N N M2B 211 Box Gene 8; Paired Domain Gene 8 PENK PENK-
HB- ppENK/ Y N M1B 163 Proenkephalin precursor PGR PGR- HB-
Progesterone Y N M1B 149 Receptor A; PR; NR3C3 PITX2 PITX2- HB-
Paired- Y N M2B 235 like 27 homeodomain transcrip- tion factor 2;
IRID2; IHG2; RIEG; RGS; IGDS PLAGL1 PLAGL1- HB- Pleiomorphic N N
M1B 199 adenoma gene-like 1; LOT1 PMS2 PMS2- HB- Postmeiotis N N
M1B 098 segregation increased 2 (S. cerevisiae); PMSL2 POLD1 POLD1-
HB- Polymerase N N M1B 139 (DNA directed), delta 1, catalytic
subunit (125 kD); POLD PPARG PPARG- HB- Peroxisome N N M1B 060
proliferative activated receptor, gamma isoform 1; PPARG1; PPARG2;
NR1C3 PRKAR1A PRKAR1A- HB protein N N MIB 214 kinase, cAMP-
dependent, regulatory, type I, alpha (tissue specific
extinguisher
1); TSE; PRKAR1 PSAT1 PSA51- HB- Phosphoserine N N M1B 231
aminotrans- ferase 1 PSEN1 PSEN1- HB- Presenilin N N M1B 262 1
(Alzheimer disease 3); AD3 PSEN2 PSEN2- HB- Presenilin N N M1B 264
2 (Alzheimer disease 4); AD4 PTEN PTEN- HB- Phosphatase N N M1B 157
and tensin homolog (mutated in multiple advanced cancers 1); MMAC1;
BZS; MHAM PTGS2 PTGS2- HB- prostaglandin- N N M1B 065 endoperoxide
synthase 2 (prostaglandin G/H synthase and cyclooxy- genase); COX2
PTTG1 PTTG1- HB- Pituitary N N M1B 052 tumor- transforming 1;
TUTR1; SECURIN; PTTG; HPTTG PYCARD PYCARD- HB- PYD and Y N M1B 228
CARD domain containing; ASC; TMS1; CARD5; MGC103 32 RAD23A RAD23A-
HB- RAD23 N N M1B 101 (S. cerevisiae) homolog A; HHR23A RARB RARB-
HB- retinoic Y N M1B 176 acid receptor, beta; HAP; RRB2; NR1B2
RARRES1 RARRES1- HB- Retinoic Y N M1B 322 acid receptor responder
(tazarotene induced) 1; TIG1 RASSF1 RASSF1A- HB- Ras Y N M1B 044
association (RaIGDS/ AF-6) domain family NORE2A; REH3P21; RDA32 RB1
RB1- HB- Retino- N N M1B 245 blastoma (including osteo- sarcoma);
OSRC RBP1 RBP1- HB- Retinol Y N M1B 185 binding protein 1, cellular
RNR1 RNR1- HB- Ribosomal N N M1B 071 RNA RPA2 RPA2- HB- Replication
Y N M1B 103 protein A2 (32 kD) RPA3 RPA3- HB- Replication Y N M1B
104 protein A3 (14 kD) RUNX3 RUNX3- HB- Runt- Y Y M1B 181 related
transcription factor (acute myeloid leukemia 1; aml1 oncogene);
CBFA3; AML2 S100A2 S100A2- HB- S100 N N M1B 061 calcium binding
protein A2; S100L; CAN19 SASH1 H-SASH1- HB- SAM and N N M1B 220 SH3
domain containing; KIAA0790 SCAM-1 SCAM-1- HB- Vinexin Y N M1B 064
beta (SH3- containing adaptor molecule- 1 SCGB3A1 SCGB3A1- HB-
Secretoglobin, Y N M1B 194 family 3A, member 1; HIN-1; HIN1; LU105;
UGRP2 SERPINB5 SERPINB5- HB- Serine (or N N MIB 208 cysteine)
proteinase inhibitor, clade B (ovalbumin), member 5; PI5; MASPIN
SEZ6L SEZ6L- HB- Seizure Y N M1B 184 related 6 homolog (mouse)-
like; KIAA0927 SFN SFN- HB- Stratifin; N N M1B 174 14-3-3 sigma
SFRP1 SFRP1- HB- Secreted Y N M1B 201 frizzled- related protein 1;
FRP-1; SARP2 SFRP2 SFRP2- HB- Secreted Y N M1B 280 frizzled-
related protein 2; FRP-2; SARP1; SDF-5 SFRP4 SFRPR- HB- Secreted Y
N M1B 281 frizzled- related protein 4; FRP-4 SFRP5 SFRPT- HB-
Secreted Y N M1B 282 frizzled- related protein 5; SARP3 SLC6A20
SLC6A20- HB- solute Y N M1B 079 carrier family 6 (proline IMINO
transporter), member 20; XT3 SMAD2 SMAD2- HB- SMAD, N N M1B 275
mothers against DPP homolog 2 (Drosophila); MADH2 SMAD3 SMAD3- HB-
SMAD, N N M1B 053 mothers against DPP homolog 3 (Drosophila); MADH3
SMAD4 SMAD4- HB- SMAD, N N M1B 277 mothers against DPP homolog 4
(Drosophila); MADH4; DPC4 SMAD6 SMAD6- HB- SMAD, Y N M1B 278
mothers against DPP
homolog 6 (Drosophila); MADH6; Hs17432 SMAD9 SMAD9- HB- SMAD, N N
M1B 315 mothers against DPP homolog 9 (Drosophila); MADH9 SMUG1
SMUG1- HB- Single- N N M1B 086 strand selective monofunc- tional
uracil DNA glycosylase SOCS1 SOCS1- HB- Suppressor Y Y M1B 042 of
cytokine signaling 1; SOCS- 1; SSI-1; JAB; TIP3; Cish1 STAT1 STAT1-
HB- Signal N N M1B 063 transducer and activator of transcription 1,
91 kDa; STAT91 STK11 STK11- HB- Serine/ N N M2B 183 threonine
kinase 11 (Peutz- Jeghers syndrome); PJS; LKB1 SYK SYK- HB- Spleen
N N M2B 241 tyrosine kinase TERT TERT- HB- Telomerase Y N M1B 074
reverse transcriptase; TRT; TP2; TCS1; EST2 TFAP2A TFAP2A- HB-
Transcription Y N M1B 314 factor AP-2 alpha (activating enhancer
binding protein 2 alpha); AP-2; TFAP2; AP2TF TFF1 TFF1- HB- Trefoil
N N M1B 145 factor 1 (breast cancer, estrogen- inducible sequence
expressed in); BCE1; D21S21 TGFBR1 TGFBR1- HB- Transforming N N M1B
192 growth factor, beta receptor I (activin receptor type II- like
kinase, 53 kDa); ALK-5 TGFBR2 TGFBR2- HB- Transforming N N M1B 246
growth factor, beta receptor II (70/80 kDa); MFS2 THBS1 THBS1- HB-
Thrombos Y N M1B 247 pondin 1; TSP1 THRB THRB- HB- Thyroid Y N M1B
216 hormone receptor, beta (erythro- blastic leukemia viral (v-
erb-a) oncogene homolog 2, avian); ERBA2; THRB1; THRB2; NR1A2 TIMP3
TIMP3- HB- TIMP Y N M1B 167 metallo- peptidase inhibitor 3 (Sorsby
fundus dystrophy, pseudoin- flammatory); SFD TITF1 TITF1- HB-
Thyroid Y N M1B 213 transcription factor 1; NKX2A; BCH; TTF-1
TMEFF2 TMEFF2- HB- Transmem- Y N M1B 274 brane protein with EGF-
like and two follistatin- like domains 2; TENB2 TNFRSF10A
TNFRSF10A- HB- Tumor N N M1B 306 necrosis factor receptor
superfamily, member 10a; DR4; Apo2; TRAILR- 1; CD261 TNFRSF10B
TNFRSF10B- HB- Tumor N N M1B 307 necrosis factor receptor
superfamily, member 10b; DR5; KILLER; TRICK2A; TRAIL- R2; TRICKB;
CD262 TNFRSF10C TNFRSF10C- HB- Tumor Y N M1B 308 necrosis factor
receptor superfamily, member 10c, decoy without an intracellular
domain; DcR1; TRAILR3; LIT; TRID; CD263 TNFRSF10D TNFRSF10D- HB-
Tumor N N M1B 309 necrosis factor receptor superfamily, member 10d,
decoy with truncated death domain; DcR2; TRUNDD; TRAILR4; CD264
TNFRSF25 TNFRSF25- HB- TNF N N M1B 080 receptor superfamily member
25; TNFRSF1 2; DR3; APO-3 TP53 TP53- HB- Tumor N N M1B 217 protein
p53 TP73 TP73- HB- Tumor Y N M1B 177 protein p73 TSHR.sup.d TSHR-
HB- Thyroid Y N M1B 141 stimulating hormone receptor; LGR3 TWIST1
TWIST1- HB- Twist Y N M1B 047 homolog (acrocepha- losyndactyly
3;
Saethre- Chotzen syndrome) (Drosophila) TYMS TYMS- HB- Thymidylate
N N M1B 248 Synthase UNG UNG- HB- Uracil- N N M1B 082 DNA
glycosylase; DGU; UDG; UNG1 UQCRH UQCRH- HB- Ubiquitinol N N M1B
224 cytochrome c reductase hinge protein VDR VDR- HB- Vitamin D N N
M1B 068 (1,25- dihydroxy vitamin D3) receptoR; NR1I1 VHL VHL- HB-
Von N N M1B 191 Hippel- Lindau syndrome tumor suppressor; VHL1 XAB2
XAB2- HB- XPA N N M1B 115 binding protein 2; HCNP XPA XPA- HB-
Xeroderma N N M1B 115 pigmentosum, comple- mentation group A; XPAC;
XP1 XPC XPC- HB- Xeroderma N N M1B 100 pigmentosum, comple-
mentation group C; XPCC XRCC1 XRCC1- HB- X-ray N N M1B 092 repair
complementing defective repair in Chinese hamster cells; RCC COL2A1
COL2A1- HB- Collagen, N/A N/A C1B 057 type II, alpha 1 (primary
osteo- arthritis, spondy- loepiphy- seal dysplasia, congenital) ALU
ALU- HB- Interspersed N/A N/A C4M 313 ALU repeat sequence HUGO Gene
Name Forward Reverse (If Chromosomal Primer Primer Probe Oligo
Available) Location Sequence Sequence Sequence.sup.a Source ABCB1
7q21.1 TCGGGTCGG CGACTATAC 6FAM- Ehrlich, M. et GAGTAGTTAT
TCAACCCAC ACGCTATTCC al. Oncogene TTG GCC TACCCAACCA 21, 6694-
ATCAACCTCA- 6702 (2002) BHQ-1 APC 5q21- GAACCAAAAC TTATATGTC 6FAM-
Eads, C. A. et q22 GCTCCCCAT GGTTACGTG CCCGTCGAAA al. Cancer
CGTTTATAT ACCCGCCGA Res 61, TTA-BHQ-1 3410-3418 (2001) APEX1
14q11.2- CGTATTTGTA GCGCATTCT 6FAM- GenBank q12 TCGGTTCGAT
TCGACCACG CAAACGCGC Number GGTA CTCTAATCAC AL355075; GTAACCAAAT-
Amplicon BHQ-1 Location: 64818-64684 APP 21q21.2 AACGAAATGC
TCGTCCCCG 6FAM- GenBank GGATAAAAAC TAAACTTAA CCCGCAAAC Number GTAT
ATCATC CTCCCGAAAA D87675; TATCGTATAA Amplicon A-BHQ-1 Location:
8572-8667 ARF/ 9p21 ACGGGCGTTT CCGAACCTC 6FAM- Eads, C. A. et
CDKN2A TCGGTAGTT CAAAATCTC CGACTCTAAA al. Cancer GA CCCTACGCAC Res
61, GCGAAA- 3410-3418 BHQ-1 (2001) ARP 7q22.1 TGCGCGGGT ACCTAAAAC
6FAM- GenBank C1B ATCGGTAGTA AACGATCGC CAAATCCCGC Number T GAAAT
CCTCCCTTCG AC004922; AAAT-BHQ-1 Amplicon Location: 57135-57206 ATM
11q22- ACGGAGAAAA GCGACGATA 6FAM- GenBank q23 GAAGTCGTG ACTACAACG
CGACTCCTCT Number GTC CAAAT CGCCTCCTCC U82828; CG-BHQ-1 Amplicon
Location: 10785-10854 ATR 3q22- AGCGGTTTTC GAATTCCCG 6FAM- GenBank
q24 GGGAGGAGT ACGTCTCCA CGACGCCCG Number AA ACGAAACCGT AC134509;
ATAA-BHQ-1 Amplicon Location: 59222-59312 AXIN1 16p13.3 CGGTTTTTGT
CGACGCGAT 6FAM- GenBank AGTTGTTTCG AACCGCTTA ATCCGAAACC Number TGTT
AA TCGAACGCGT AE006463; CTCG-BHQ-1 Amplicon Location: 84738-84807
BCL2 18q21.3 TCGTATTTCG AACTAAACG 6FAM- Widschwendter, GGATTCGGTC
CAAACCCCG ACGACGCCG M. et al C AAAACAACCG Cancer Res AAATCTACA-
64,3807- BHQ-1 3813 (2004) BDNF 11p13 CGTATCGGGT CGCCCGCTC 6FAM-
GenBank TGGTTTTTTT GCTATCC CCGTAACGC Number GTT CTCGAACTCC
AC103796; CGA-BHQ-1 Amplicon Location: 3794-3866 BRCA1 17q21
GAGAGGTTGT CGCGCAATC 6FAM- Fiegl, H. et al TGTTTAGCGG GCAATTTTA
CCGCGCTTTT Cancer TAGTT AT CCGTTACCAC Epidemiol GA-BHQ-1 Biomarkers
Prev 13, 882- 888 (2004) BRCA2 13q12.3 CGTTACGGC CCGCCTCTA 6FAM-
GenBank GTTACGTGGT CCGCCTAAT CGCGCCACA Number TT AACCCGCG-
AL445212; BHQ-1 Amplicon Location: 83637-83703 CACNA1G 17q22
TTTTTTCGTTT CTCGAAACG 6FAM- GenBank CGCGTTTAGG ACTTCGCCG AAATAACGCC
Number T GAATCCGACA AC021491; ACCGA-BHQ-1 Amplicon Location:
48345-48411 CALCA 11p15.2- GTTTTGGAAG TTCCCGCCG 6FAM- Eads, C.A. et
p15.1 TATGAGGGTG CTATAAATC ATTCCGCCAA al. Cancer ACG G TACACAACAA
Res 61, CCAATAAACG- 3410-3418 BHQ-1 (2001) CCND1 11q13 GGTAATTTCG
GAACGCCAA 6FAM- GenBank TCGTAGGGTA ACGCCGA ACCCAAAAAC Number GGC
CATCCCTAAA AF511593; ACGCCG- Amplicon BHQ-1 Location: 1211-1293
CCND2 12p13 GGAGGGTCG TCCTTTCCC 6FAM- Ehrlich, M. et GCGAGGAT
CGAAAACAT CACGCTCGAT al. Oncogene AAAA CCTTCGCCC 21, 6694- G-BHQ-1
6702 (2002) CDH1 16q.22.1 AGGGTTATCG TTCACCTAC 6FAM- GenBank
CGTTTATGCG CGACCACAA ACTAACGACC Number CCA CGCCCACCC AC099314;
GA-BHQ-1 Amplicon Location: 80648-80743 CDH13 16q24.2- AATTTCGTTC
CTACCCGTA 6FAM- Fiegl, H. et al q24.3 GTTTTGTGCG CCGAACGAT
AACGCAAAAC Cancer T CC GCGCCCGAC Epidemiol A-BHQ-1 Biomarkers Prev
13, 882- 888 (2004) CDK2AP1 12p14. CGCGGAAAG CGCACTTTT 6FAM-
GenBank TTTGCGGT TATTATCGA CGACAAATAT Number CGACTC AACCGTCCG
AC068768; CGCCCTA- Amplicon BHQ-1 Location: 51406-51526 CDKN1A
6p21.2 CGCGTTCGG TTATAATCC 6FAM- GenBank TTTGCGTAT CGCTCTCCG
AAATCTCCGA Number CC CACATCCCGA Z85996; CTCTCGT- Amplicon BHQ-1
Location: 3463-3554 CDKN1C 11p15.5 TCGAGTAGG GTCCCGAAA 6FAM-
GenBank GCGCGAATTA TCCCCGAAT AACTAATCAA Number G CGAAAAACTC
AC013791; CTAACCGCG Amplicon CT-BHQ-1 Location: 57564-57638 CDKN2A
9p21 TGGAGTTTTC AACAACGCC 6FAM- Eads, C.A. et GGTTGATTGG CGCACCTCC
ACCCGACCC al. Cancer TT T CGAACCGCG- Res 61, BHQ-1 3410-3418 (2001)
CDKN2B 9p21 AGGAAGGAG CGAATAATC 6FAM- Eads, C. A. et AGAGTGCGT
CACCGTTAA TTAACGACAC al. Cancer CG CCG TCTTCCCTTC Res 61,
TTTCCCACG- 3410-3418 BHQ-1 (2001) CDX1 5q31- TGAGCGGTT AAATCCCCC
6FAM- GenBank
q33 GTTCGTCGTC GCGCATACT CCTAAAACCG Number A CCGCTACCG AC005895;
ACCG-BHQ-1 Amplicon Location: 35199-35266 CGA 6q12- GGGTTTTTTG
AACTACAAT 6FAM- GenBank q21 TAGGATGTGT TACTAAAAA TCCCTCTTCG Number
TTAGG CTCATAAAA AATCCACAAT AL138827; CGAAACT CAACCG- Amplicon BHQ-1
Location: 56007-56096 CHFR 12q24.33 CGGGAGTTTT AACCGTCCC 6FAM-
GenBank TATGGGCGT CAAAACTAC CCTCGAACC Number GAC GCTCCATCGA
AC127070; AATTCA-BHQ- Amplicon 1 Location: 62442-62545 CLDN1
3q28-q29 CGGTGAGTC ACGCAAAAC 6FAM- GenBank GTTTTGAAAT CGCTAAACG
GATTTAAAAC Number CG C AACTCCGCCC AC009520; GCCTCA-BHQ- Amplicon 1
Location: 27434-27530 CLIC4 1p36.11 GGCGGTGTT CCGATTCCC 6FAM-
GenBank GAGGAGTTG GCCGTACTA CGCTAAACTA Number A C TCCGAAATCG
AL117424; AACTAACCAC Amplicon G-BHQ-1 Location: 47- 119 COL1A2
7q22.1 CGGTAGTAG CCTAAATCA 6FAM- GenBank GAGGTTTCG CCGACGAAA
CGAACGCGA Number GTTAAGT ATATCA ACATACAATC AF004877; GTAACCAATA
Amplicon CCT-BHQ-1 Location: 2302-2390 CRABP1 15q24 TCGAAATTTT
TATCCGTAC 6FAM- GenBank CGTTGTTGCG CTACCGCCG ACCATACCCA Number T C
ACTTCGCCGA AC011270; CACCTAA- Amplicon BHQ-1 Location: 122142-
122223 CTNNB1 3p22- GGAAAGGCG TCCCCTATC 6FAM- Eads, C.A. et 21.3
CGTCGAGT CCAAACCCG CGCGCGTTTC al. Cancer CCGAACCG- Res 61, BHQ-1
3410-3418 (2001) CTSD 11p15.5 TACGTTTCGC TCGTAAAAC 6FAM- GenBank
GTAGGTTTGG GACCCACCC CCTATCCCGA Number A TAA CCGCCGCGA- AC068580;
BHQ-1 Amplicon Location: 43076-43166 CXADR 21q11.2 TACGCGGTTG
ATAAACTCG 6FAM- Ehrlich, M. et GAGAAGTCG CGTCACTTC AACGACCCG al.
Oncogene CGA AACCGAACTA 21, 6694- CGAACG- 6702 (2002) BHQ-1 CYP1B1
2p21 GTGCGTTTGG AACGCGACC 6FAM- Fiegl, H. et al ACGGGAGTT TAACAAAAC
CGCCGCACA Cancer GAA CCAAACCGCT Epidemiol T-BHQ-1 Biomarkers Prev
13, 882- 888 (2004) CYP27B1 12q14.1 GGGATAGTTA CCGAATATA 6FAM-
GenBank GAGAGAACG ACCACACCG CCAACCTCAA Number GATGTTT CC CTCGCCTTTT
AY288916; CCTTATTTCA- Amplicon BHQ-1 Location: 1728-1805 DAPK1
9q34.1 TCGTCGTCGT TCCCTCCGA 6FAM- Muller, H. M. TTCGGTTAGT
AACGCTATC CGACCATAAA et al. Cancer T G CGCCAACGC Lett209, 231-
CG-BHQ-1 236 (2004) DCC 18q21.3 GGGTTCGGC CGAAAAATA 6FAM- GenBank
GCGTGT CAAAAACCA ACCAAAAATC Number ACTTAAATA GCGAACAAC AC011155; CC
GACAACACT- Amplicon BHQ-1 Location: 118286- 118444 DCLRE1C 10p13
CGAAGCGCG AAAATCCGA 6FAM- GenBank GGTGATTTA AAACCGAAA ATCCGATCGA
Number ACAA ATTCTAAACG AL360083; CCCGCTACT- Amplicon BHQ-1
Location: 54518-54603 DDB1 11q12- GGGCGGAGG CCCGTCGAA 6FAM- GenBank
q13 TAGCGGT ACTCGAACG CCAACAACGC Number GCAACGAACT AC090584;
CCA-BHQ-1 Amplicon Location: 203224- 203324 DIRAS3 1p31 GCGTAAGCG
CCGCGATTT 6FAM- Previously GAATTTATGT TATATTCCG CGCACAAAAA
described as TTGT ACTT CGAAATACGA ARHI in AAACGCAAA- Fiegl, H. et
al BHQ-1 Cancer Epidemiol Biomarkers Prev 13, 882- 888 (2004) DLC1
8p22- AGTAAGGATG ACGACTCGA 6FAM- GenBank p21.3 CGTTGAGGAT CTTCCGCGT
AACCCACGAC Number CG C GACACCCGA AC015641; AACG-BHQ-1 Amplicon
Location: 115709- 115784 DLEC1 3p22- TCGTTGCGTA CGTAACGCT 6FAM-
GenBank p21.3 TTTAAGATAT CATTCTCGC TAATCAAACT Number TTCGTATT TACC
TACGCTCACT AP006309; TCGTCGCCG- Amplicon BHQ-1 Location:
19959-20088 DNAJC1 13q13 TTTCGGGTCG ACTACAAAT 6FAM- Previously
TTTTGTTATG ACTCAACGT TCGCCAACTA described as G AACGCAAAC AAACGATAAC
MCJ in T ACCACGAACA- Ehrlich, M. et BHQ-1 al. Oncogene 21, 6694-
6702 (2002) DPH1 17p13.3 ACGCGGAGA CCGCCCAAC 6FAM- GenBank
GCGTAGATAT GAATATCCC CCCGCTAACC Number TG GATCGACGAT AC090617;
CGA-BHQ-1 Amplicon Location: 196988- 197057 DRD1 5q35.1 GGCGCGCGT
TACCCGTAA 6FAM- GenBank TGGTTC ACGCCTATA CTCGCAAAAA Number CTCACC
AAACGCGAC AC091393; GCAACTA- Amplicon BHQ-1 Location: 111358-
111429 DRD2 11q23 GAAGTCGGA ATCTCGAAA 6FAM- GenBank AATTTTGGTC
AAACACTTC ACACCCAAAC Number GC CCCC GCGAAACCC AP002840; GAAACT-BHQ-
Amplicon 1 Location: 110939- 111008 EBF3 10q26 GTAGGATATT GCAACACTC
6FAM- GenBank GCGGGATCG ACTACCCCG TCTTTAAAAC Number TTC TTTAT
AAACGAACCG AL354950; CGCCAA- Amplicon BHQ-1 Location: 144175-
144252 EPM2AIP 3p21.3 CGTTATATAT CTATCGCCG 6FAM- Eads, C. A. et
CGTTCGTAGT CCTCATCGT CGCGACGTC al. Cancer ATTCGTGTTT AAACGCCACT Res
61, ACG-BHQ-1 3410-3418 (2001) ERBB2 17q21.1 AGTGTGAGAA CCCTCTCTT
6FAM- GenBank CGGTTGTAG CGCGCAAAC AAATACGTCC Number GTAATTTAG
CTCCTAACGC AC079199; CGAAACG- Amplicon BHQ-1 Location: 44277-44352
ERCC1 19q13.2- GGGCGAGTC CTCCGAAAA 6FAM- GenBank q13.3 GAAGGTGG
CTCCATAAC CCCAACGCTA Number GTCAA AAAACTCTAT M63796; AACGCCACG-
Amplicon BHQ-1 Location: 22178-22088 ERCC2 19qq13.3 CGAGTTTTCG
CCGACCGAA 6FAM- GenBank AGGATGTTTA CTATACAAC ACCCGCCTC Number CGA
GAAAT CCTCATAAAT AC092309; ATTCAACGAA- Amplicon BHQ-1 Location:
4166-4250 ERCC4 16p13.3- TCGACGGATT CCGTCAATA 6FAM- GenBank p13.11
GTTATGGCG TCGAACAAT CACCAACTAT Number TCCA CGCTCGTACT L76568;
CCAACAACG- Amplicon BHQ-1 Location: 2113-2184 ERCC5 13q22
TAAGCGTAGA CCCGCTCGA 6FAM- GenBank AAATATACGT TTTCCGTCT CGACGCGCA
Number TATGTGCG AAACGAAAAC AL157769; TCCG-BHQ-1 Amplicon Location:
130480- 130556 ERCC6 10q11 ACGTAAGTAG CGACTCCGA 6FAM- GenBank
AAAGGCGTTG CTTCTACTA CCCGTAACG Number TTGAG ATACGAAA CATACGCCTA
AC073366; ACTCAACG- Amplicon BHQ-1 Location: 164190- 164315 ERCC8
5q12.1 GGTTAAGGC TCATACGAC 6FAM- GenBank GTTTAGAGTC ACTTAAAAT
CCCTTCACTC Number GGG ATCACCGAA TAACATCGAA AC073546; A ACCCTACCCG-
Amplicon BHQ-1 Location: 21088-21200 ESR1 6q25.1 GGCGTTCGTT
GCCGACAC 6FAM- Eads, C. A. et TTGGGATTG GCGAACTCT CGATAAAACC al.
Cancer AA GAACGACCC Res 60, GACGA-BHQ-1 5021-5026 (2000) ESR2 14q
TTTGAAATTT ACCCGTCGC 6FAM- Fiegl, H. et al GTAGGGCGA AACTCGAAT
CCGACCCAA Cancer AGAGTAG AA CGCTCGCCG- Epidemiol BHQ-1 Biomarkers
Prev 13,882- 888 (2004) FAF1 1p33 CGTTTTGCGG CAACGCAAA 6FAM-
GenBank TTTTACGTGA AATCCTAAC CGCGCGCTC Number CGAA AACGCTTAAC
AL359977; AAAAAAATA- Amplicon BHQ-1 Location: 63234-63308
FBXW7 4q31.23 TGTCGTTGCG CGAAAATAA 6FAM- GenBank GTTGGGAT ATAACTACT
ACGCCAAAAC Number CCGCGATAA TTCTACCTCG AC023424; TCCCGTAA- Amplicon
BHQ-1 Location: 113422- 113545 FHIT 3p14.2 GGCGCGGGT CGCCCCGTA
6FAM- GenBank TTGGG AACGACG CACTAAACTC Number CGAAATAATA AC097357;
ACCTAACGCG Amplicon CG-BHQ-1 Location: 121650- 121724 GABRA2 4p12
TCGTCGGAG AACCTCTCG 6FAM- GenBank GAGCGGA AAAACCCCA ACGACCTCGA
Number ACA AAAACAACCC AC095060; GAAACTACG- Amplicon BHQ-1 Location:
42382-42455 GAD1 2q31 CGATTGGTTC CCCTCCGAT 6FAM- GenBank GGCGTAGAA
ATACAAAAC CCCGCACAA Number A CCC CTCTCGCTTC AC007405; TCTTTACAA-
Amplicon BHQ-1 Location: 70850-70932 GATA3 10p15 TGTATCGGGA
ACGCGCGCT 6FAM- GenBank CGGAATCGTT CTAACCCTT AAATATAACC Number
GCGACTCCTA AL390294; CCAATTCATT Amplicon CG-BHQ Location:
51880-51959 GATA4 8p23.1- GATGGTGGT TTCCCTCCA 6FAM- GenBank 8p22
CGCGTGAAG TATACGAAC CCTATCCCGA Number TTA TACCG ATCCGTCAAT
AC069185; CCCG-BHQ-1 Amplicon Location: 28557-28630 GATA5 20q13.33
AGTTACGTGA TAATCCGAA 6FAM- GenBank TTTTGGTAGG CTCCGCGCT CCCGTATCGT
Number TTTTGTT A ACGTCCTTAT AL499627; CGCCAAA- Amplicon BHQ
Location: 19744-19828 GDNF 5p13.1- CGGTAGTTGT AACAACCGC 6FAM-
GenBank p12 CGTTGAGTCG CGCTACTTT CGCGCGTCG Number TTC AAATA
CGCTCTTAAC AC008869; TAAAA-BHQ-1 Amplicon Location: 108758- 108866
GRIN2B 12p12 GTCGGATTTA CTACCGCCG 6FAM- GenBank CGCGTCGAG CGCTAAAAT
ACGCACGAAA Number T AC CTTCACCTAC AC007916; AACGTATCG- Amplicon
BHQ-1 Location: 111645- 111727 GSTP1 11q13 GTCGGCGTC AAACTACGA
6FAM- Eads, C. A. et GTGATTTAGT CGACGAAAC AAACCTCGCG al. Cancer
ATTG TCCAA ACCTCCGAAC Res 61, CTTATAAAA- 3410-3418 BHQ-1 (2001)
HIC1 17p13.3 GTTAGGCGG CCGAACGCC 6FAM- Eads, C. A. et TTAGGGCGTC
TCCATCGTA CAACATCGTC al. Cancer T TACCCAACAC Res 61, ACTCTCCTAC
3410-3418 G-BHQ-1 (2001) HLA-G 6p21.3 CACCCCCATA GGTCGTTAC 6FAM-
Muller, H. M. TACGCGCTAA GTTTCGGGT CGCGCTCAC et al Ann NY AGTTTA
ACGCTCAAAA Acad Sci ACCT-BHQ-1 1022, 44-49 (2004) HOXA1 7p15
TTGTTTATTA TCGAACCAT 6FAM- GenBank GGAAGCGGT AAAATTACA TCGTACGCGA
Number CGTC ACTTTCCA TCAACGCCAA AC004079; CAATTA-BHQ- Amplicon
Location: 78138-78220 HOXA10 7p15- TGTATTGATG CCCACCAAC 6FAM-
GenBank p14 GGTTAGGAG CACGTTAAA CAACTCCCGA Number ACGTATT ACA
CCTTCGAACC AC004080; AAAATATCG- Amplicon BHQ-1 Location:
47850-47933 HOXA11 7p15- TTTTGTTTTC TAATCAAAT 6FAM- GenBank p14
GATTTTAGTC CACCGTACA ACCACCAAAC Number GGAAT AATCGAAC AAACACATCC
AC004080; ACGACTTCA- Amplicon BHQ-1 Location: 59150-59249 HRAS
11p15.5 GAGCGATGA CGTCCACAA 6FAM- Widschwendter, CGGAATATAA
AATAATTCTA CACTCTTACC M. et al GTTGG AATCAACTA CACACCGCC Cancer Res
A GACG-BHQ-1 64, 3807- 3813 (2004) HSD17B4 5q21 TATCGTTGAG
TCCAACCTT 6FAM- Muller, H. M. GTTCGACGG CGCATACTC CCCGCGCCG et al.
Cancer G ACC ATAACCAATA Lett 209, CCA-BHQ-1 231-236 (2004) ICAM1
19p13.3- GGTTAGCGA TCCCCTCCG 6FAM- Ehrlich, M. et p13.2 GGGAGGATG
AAACAAATA TTCCGAACTA al. Oncogene ATT CTACAA ACAAAATACC 21, 6694-
CGAACCGAAA- 6702 (2002) BHQ-1 IFNG 12q14 TGAAGAGTTA TTCCTTTAAA
6FAM- GenBank ATATTTTATTA CTCCTTAAA ACAAACCCAT Number GGGCGAA
TCCTTTAAC TATACCCACC AF375790; G TA-MGBNFQ Amplicon Location:
1245-1407 IGF2 11p15.5 GAGCGGTTTC CCAACTCGA 6FAM- GenBank
GGTGTCGTTA TTTAAACCG CCCTCTACCG Number ACG TCGCGAACC AC132217;
CGA-BHQ-1 Amplicon Location: 108633- 108720 IGSF4 11q23.2 GGGTTTCGG
CACTAAAAT 6FAM- Widschwendter, AGGTAGTTAA CCGCTCGAC ACACTCGCCA M.
et al CGTC AACAC TATCGAACAC Cancer Res CTACCTCAAA- 64, 4472- BHQ-1
4480 (2004) ITGA4 2q31- TGCGGAGGC CAACCGAAA 6FAM- GenBank q32
GTAGGGTC TTCCCCAAC CCTACAACCG Number G CGCGTAAACA AC020595;
AAAACG-BHQ- Amplicon 1 Location: 146569- 146639 JUP 17q21 GGATAGCGA
CTCTTCGCC 6FAM- GenBank ATTGAGTTCG TTTTATTCGA AACAACCGCC Number GC
TTACTAAAT GCCCGACCA- AC109319; BHQ-1 Amplicon Location: 81609-81699
KL 13q12 AGTTTGGTTT CGCCCGACT 6FAM- GenBank TCGCGTAGTA CCGCAC
CGAACGACG Number TGTTC CGACGAAAC AB009667; GCT-BHQ-1 Amplicon
Location: 2062-2189 LDLR 19p13.3 GATATCGGTT TTCACCGAA 6FAM- GenBank
TTTTAATTCG AACCCAAAT ATCAAATCGC Number TGAAGTT ACAA CTACCCTAAC
AC011485; GACACTTTCG- Amplicon BHQ-1 Location: 90572-90674 LIG3
17q11.2- GTCGCGGGT CGACCTTAA 6FAM- GenBank q12 AGTTTACGAC CTCTTACGC
CGCTACCTCC Number G CTACG CGCTCTAAAA AC022903; CCCGA-BHQ-1 Amplicon
Location: 14538-14629 LPHN2 1p31.1 GAGGATTTAG AATCCCCGA 6FAM-
GenBank CGCGTAGTG ACTCTACCT CCCATTAACA Number AGTG CCA CACCCATTCA
AL358939; ACCGCTAA- Amplicon BHQ-1 Location: 143237- 143336 LZTS1
8p22 GCGGCGTTG CGCGCGCTA 6FAM- GenBank TAGGGACG ACTCTTCTA
ATTACCGCCT Number CG TTAAACTCCG AC025853; AACCCTCCA- Amplicon BHQ-1
Location: 24463-24547 MBD2 18q21 AGGCGGAGA CCCTCCTAC 6FAM- GenBank
TAAGATGGTC CCGAAACGT CGACCACCG Number GT AAC CCTCTTAAAT AC093462;
CCTCCAAA- Amplicon BHQ-1 Location: 143589- 143667 MBD4 3q21-
TCGTGTTTAT TCGATTACA 6FAM- GenBank q22 CGAGTAGGG ACCCGATAC
CACACCCTAA Number TTCG CGTAA ACGTTACGAC AF449212; GCTAAACTCG-
Amplicon BHQ-1 Location: 59852-59967 MGMT 10q26 GCGTTTCGAC
CACTCTTCC 6FAM- Virmani, A. K. GTTCGTAGGT GAAAACGAA CGCAAACGAT et
al. Cancer ACG ACGCACCGC Epidemiol GA-BHQ-1 Biomarkers Prev11, 291-
297 (2002) MINT1 5q13- GGGTTGAGG CCCCTCTAA 6FAM- GenBank 14
TTTTTTGTTA ACTTCACAA CTACTTCGCC Number GCG CCTCG TAACCTAACG
AC026774; CACAACAAAC Amplicon G-BHQ-1 Location: 44288-44415 MINT2
2p22- TTGAGTGGC TCCCCGCCT 6FAM- GenBank 21 GCGTTTCGT AAACCAACC
CTTACGCCAC Number CGCCTCCGA- AC007238; BHQ-1 Amplicon Location:
74436-74524 MINT31 17q22 GTCGTCGGC CACCGACGC 6FAM- GenBank
GTTATTTTAG CCAACACA ACGCTCCGCT Number AAAGTT CCCGAATACC AC021491;
CA-BHQ-1 Amplicon Location: 50060-50130 MLH1 3p21.3 AGGAAGAGC
TCTTCGTCC 6FAM- Fiegl, H. et al GGATAGCGAT CTCCCTAAA CCCGCTACCT
Cancer TT ACG AAAAAAATAT Epidemiol ACGCTTACGC Biomarkers G-BHQ-1
Prev 13, 882- 888 (2004) MLH3 14q24.3 TGATGATGGT CGACCGCCA 6FAM-
GenBank TGCGCGTAGT AACCGC CGAAACCCTC Number GCGCATCCG AL049780;
A-BHQ-1 Amplicon
Location: 110441- 110511 MMS19L 10q24- TTAGGTAGAA ATAACTCGA 6FAM-
GenBank 10q25 GTCGGTAGG AACGAACTC CGCCTCCCG Number TACGTGA TCCGC
AACCAATCTC AL359388; CG-BHQ-1 Amplicon Location: 11495-11598 MSH2
2p22- TTTTAGTGCG AAACGATCC 6FAM- GenBank p21 GAGGTACGG TCCGAAACC
CCGCACAAAC Number G AAA ACCAACGTTC AC079775; CG-BHQ-1 Amplicon
Location: 98483-98569 MSH4 1p31 CGGATTTTAG CCGATCGCC 6FAM- GenBank
GAGATTTTAT CGCAAC AACGTACCAA Number AGAGTCG AACAAATAAA AL359206;
TACAAAAACC Amplicon ACCTAAACCG- Location: BHQ-1 16910-17000 MSH5
6p21.3 TTCGTGGCG CCGCCATCG 6FAM- GenBank GTCGGTTA CAACGTT
CCCGCCTTTT Number CAATAACCTA AC020768; AATCGCTACA- Amplicon BHQ-1
Location: 68650-68732 MSH6 2p16 GGAGTGTTTC CTACCGCCG 6FAM- GenBank
GGTTCGGTTA ACGCCTAAA CCCTTCCCTC Number GT ACGCCGCGA- AC006509;
BHQ-1 Amplicon Location: 34144-34228 MT1A 16q13 CGTGTTTTCG
CTCGCTATC 6FAM- GenBank TGTTATTGTG GCCTTACCT TCCACACCTA Number TACG
ATCC AATCCCTCGA AC106779; ACCCACT- Amplicon BHQ-1 Location:
18175-18254 MT1T 16q13 CGTTTAAGGG CCGCTAAAT 6FAM- GenBank
ATTTTGTATTT CCGCACCG CGCGATCCC Number GGTTTAT GACCTAAACT AC026461;
ATACGCA- Amplicon BHQ-1 Location: 19549-19625 MT2A 16q13 GCGTTTTCGT
TTCCCAAAT 6FAM- GenBank CGTGTGTATA CCCGCTTTC CGCGCGCTA Number GTTT
A ACGACTCAAA AC026461; TTCG-BHQ-1 Amplicon Location: 79477-79565
MT3 16q13 GGTTTTAGGG CCGCGCGT 6FAM- GenBank TTTATGTCGA CCAATTACT
AAAACCCGTT Number GGAGA TA CACCGCCTC AC026461; CAACTACTA- Amplicon
BHQ-1 Location: 98167-98241 MTHFR 1p36.3 TGGTAGTGAG CGCCTCATC 6FAM-
Eads, C. A. et AGTTTTAAAG TTCTCCCGA TCTCATACCG al. Cancer ATAGTTCGA
CTCAAAATCC Res 61, AAACCCG- 3410-3418 BHQ-1 (2001) MUTYH 1p34.3-
TCGGGTGGA AAAATTACC 6FAM- GenBank p32.1 TTCGAGTTAC TCCCGCGAA
CGCGCCCGA Number G CTCTA CTTTCCGACG- AL359540; BHQ-1 Amplicon
Location: 54075-54149 MYOD1 11p15.4 GAGCGCGCG TCCGACACG 6FAM- Eads,
C. A. et TAGTTAGCG CCCTTTCC CTCCAACACC al. Cancer CGACTACTAT Res
61, ATCCGCGAAA- 3410-3418 BHQ-1 (2001) NCL 2q12- CGTGTCGTTT
ACCAAAACT 6FAM- GenBank qter CGGTTCGTT CGCGACCGT CCATAAACCA Number
C ATCGCGAACC M60858; TCTAACCGT- Amplicon BHQ-1 Location: 886-975
NEUROD1 2q32 GTTTTTTGCG CCGCGCTTA 6FAM- GenBank TGGGCGAAT ACATCACTA
CGCGCGACC Number ACTAAA ACGACACGAA AC013733; A-BHQ-1 Amplicon
Location: 78576-78657 NEUROD2 17q12 GGTTTGGTAT ACGAACGCC 6FAM-
GenBank AGAGGTTGGT GACGTCTTC CGCCATACGA Number ATTTCGT ACCGCGAAA
AC087491; CGAATATAA- Amplicon BHQ-1 Location: 38463-38551 NEUROG1
5q23- CGTGTAGCGT CGATAATTA 6FAM- GenBank q31 TCGGGTATTT CGAACACAC
CGATAACGAC Number GTA TCCGAAT CTCCCGCGA AC005738; ACATAAA- Amplicon
BHQ-1 Location: 75342-75429 NR3C1 5q31 GGGTGGAAG AAACTTCCG 6FAM-
GenBank GAGACGTCG AACGCGCG GTCCCGATCC Number TAG CAACTACTTC
AY436590; GACCG-BHQ-1 Amplicon Location: 1786-1861 NTF3 12p13
TTTCGTTTTT CCGTTTCCG 6FAM- GenBank GTATTTTATG CCGTAATAT TCGCCACCAC
Number GAGGATT TC GAAACTACCC AC135585; ACG-BHQ-1 Amplicon Location:
7503-7576 NTHL1 16p13.3 CGGGACGTC CCGACCTTT 6FAM- GenBank GTCGGAAG
CCGCCAAA CGACCCTCC Number GCGCAATACC AC005600; G-BHQ-1 Amplicon
Location: 24563-24676 OGG1 3p26.2 TAGGGTGGG CCGCGAAAC 6FAM- GenBank
CGGGTCG GCCCAA CAATACCGAC Number CAACCGCGC AJ131341; GA-BHQ-1
Amplicon Location: 1723-1854 ONECUT2 18q21.1- ACGGGCGTT CCACAACCA
6FAM- GenBank 18q21.2 AAGCGTAATT CTAATAACT CCCGCCTCC Number ATTT
TCCCGTA CGAAACAACT AC090340; ACGA-BHQ-1 Amplicon Location:
75559-75639 OPCML.sup.b 11q25 CGTTTCGAGG CGAACCGCC 6FAM- GenBank
CGGTATCG GAAATTATC AACAACTCCA Number AT TCCCTAACCG AC027631;
CCACTTTCT- Amplicon BHQ-1 Location: 157489- 157560 PARP1.sup.c
1q41- CGGGTTTAG AAACGACCG 6FAM- GenBank q42 GGAGCGAGC CGAACCCAT
CGCTCCGAAA Number A ACCCGAACC AL359704; GAA-BHQ-1 Amplicon
Location: 146947- 147015 PARP2 14q11.2- GGGCGAGAG TCGTTCCTT 6FAM-
GenBank q12 GTTCGGAGT TCTAACTAC CCCGCATACC Number CCGC GTCCCGCGA
AL355075; TA-BHQ-1 Amplicon Location: 176220- 176286 PAX8 2q12
GTTCGTAGTT CGCATCTCA 6FAM- GenBank CGTCGAGGG TACCCTTCT CAAACGCGA
Number TTC CCTAAAT CCCGAACCTA AC016683; CGAAAA-BHQ- Amplicon 1
Location: 115663- 115758 PENK 8q23- GGTTAATTAT CAACGTCTC 6FAM-
GenBank q24 AAAGTGGTTT TACGAAATC AACGCCTACC Number TAGTAGTCGT
ACGAAC TCGCCGTCC AC012349; TAAG CG-BHQ-1 Amplicon Location:
81412-81510 PGR 11q22- GGCGGTGAC ACAAACCGT 6FAM- Woodson, K. q23
GGTCGTATTC CCCGCGAA AACAACCGCT et al Cancer CGCGCCCGA- Epidemiol
BHQ-1 Biomarkers Prev 14, 1219- 1223 (2005) PITX2 4q25- AGTTCGGTTG
TACTTCCCT 6FAM- GenBank CGCGGTT CCCCTACCT CGACGCTCG Number CGTT
CCCGAACGC AC017068; TA-BHQ-1 Amplicon Location: 117302- 117404
PLAGL1 6q24- ATCGACGGG CTCGACGCA 6FAM- GenBank q25 TTGAATGATA
ACCATCCTC ACTACCGCGA Number AATG TT ACGACAAAAC AL109755;
CCACG-BHQ-1 Amplicon Location: 52969-53045 PMS2 7p22 TCGTGGTTTG
CCTAATACA 6FAM- GenBank GCGTGGAT TCGAAATAA CCAACGATCG Number
CGCGTACC AAAACCGCCA AC005073; AACA-BHQ-1 Amplicon Location: 150898-
150982 POLD1 19q13.3 GGGACGCGG GATCTAAAC 6FAM- GenBank AGGATGC
GCCGCGATT TCCTCCCACC Number CTAT CTCGAATATT AC073646; ACGCG-BHQ-1
Amplicon Location: 123366- 123435 PPARG 3p25 GCGTTCGCG CGCCCCAAA
6FAM- GenBank TTCGTTTTC CGACGAC CCCGCCTAC Number CCGCGACGA
AC091492; AA-BHQ-1 Amplicon Location: 138096- 138211 PRKAR1A 17q23
CGGATTTGTA ACCGAACAC 6FAM- GenBank GTAGTTGCGT AAAATACGC CATCCCGACC
Number TGC GAC ATCCGCCCG- AC079210; BHQ-1 Amplicon Location:
118231- 118314 PSAT1 9q21 TGGGTTTGGT ACGTACTCC 6FAM- GenBank
TTCGTTAAGT CGCCTAAAC ACGCCCGCT Number TGT CTC CGCGAAAACT AL353594;
TACTAAATA- Amplicon BHQ-1 Location: 5511-5591 PSEN1 14q24.3
GTCGGGTGG AACACCTAC 6FAM- GenBank
AGAGAGATTT GCCCTAAAA TCGAACAAAC Number CG CGTC AACATTTCCG AF205592;
AACCAAAACT- Amplicon BHQ-1 Location: 6663-6739 PSEN2 1q31-
GAGGCGTGT CCGATACTA 6FAM- GenBank q42 AGTAGGCGG AAAACCGAA
CGCAACGAAA Number G TAAACTCG ATCTCCGACG U50871; AAAAAA-BHQ-
Amplicon 1 Location: 26196-26284 PTEN 10q23.3 GTTTCGCGTT CAATATAAC
6FAM- GenBank GTTGTAAAAG TACCTAAAA TTCCCAACCG Number TCG CTTACTCGA
CCAACCTACA AF143312; ACCG ACTACACTTA- Amplicon BHQ-1 Location:
1060-1147 PTGS2 1q25.2- CGGAAGCGT AATTCCACC 6FAM- Fiegl, H. et al
q25.3 TCGGGTAAAG GCCCCAAAC TTTCCGCCAA Cancer ATATCTTTTCT Epidemiol
TCTTCGCA- Biomarkers BHQ-1 Prev 13, 882- 888 (2004) PTTG1 5q35.1
GCGTTCGTTT CCGCGACC 6FAM- GenBank ATCGCGGT CTCCCATT ACTCACGCAA
Number ATCTTAACAA AC091842; CCGCATTCA- Amplicon BHQ-1 Location:
88212-88281 PYCARD 16p12- TTGGAGATTT ACCCTAATA 6FAM- GenBank p11.2
ACGGCGTCG CGTAACCGC CATCTCCTAC Number CTACAA AAACCCATAT AC009088;
CGCGCAA- Amplicon BHQ-1 Location: 85330-85425 RAD23A 19p13.2
TATCGATAAC GCAAACTAA 6FAM- GenBank GGGTATGGC ACTCCGCGC TTACTCGACC
Number GTT TATAA CGCACACGTA AD000092; ATCTCCTAAA- Amplicon BHQ-1
Location: 92213-92298 RARB 3p24 TTTATGCGAG CGAATCCTA 6FAM- GenBank
TTGTTTGAGG CCCCGACGA CTCGAATCGC Number ATTG TAC TCGCGTTCTC X56849;
GACAT-BHQ-1 Amplicon Location: 921-1006 RARRES1 3q25.31- GGCGAGTCG
CGCAAACTC 6FAM- GenBank 3q26.1 GATCGGAA CTACAACAA CGCGCGACG Number
ACGA CTTCACTTCT AC080013; TCAA-BHQ-1 Amplicon Location: 66080-66144
RASSF1 3p21.3 ATTGAGTTGC ACACGCTCC 6FAM- Previously GGGAGTTGG
AACCGAATA CCCTTCCCAA described as T CG CGCGCCCA- RASSF1A in BHQ-1
Ehrlich, M. et al. Oncogene 21, 6694- 6702 (2002) RB1 13q14.2
TTAGTTCGCG ACTAAACGC 6FAM- Eads, C. A. et TATCGATTAG CGCGTCCAA
TCACGTCCGC al. Cancer CG GAAACTCCCG Res 61, A-BHQ-1 3410-3418
(2001) RBP1 3q23 CGCGTTGGG GATACTACG 6FAM- GenBank AATTTAGTTG
CGAATAATA ACGCCCTCC Number TC AACGACCC GAAAACAAAA AC046134;
AACTCTACG- Amplicon BHQ-1 Location: 137423- 137512 RNR1 13p12
CGTTTTGGAG AAACAACGC 6FAM- Muller, H. M. ATACGGGTC CGAACCGAA
ACCGCCCGT et al. Cancer G ACCACACGCA Lett209, 231- AA-BHQ-1 236
(2004) RPA2 1p35 TGGCGCGAA CGTATAATC 6FAM- GenBank TTTGAGTACG
CCACCCTCG CGCGACTTCT Number TCA ACCGTCACTT AL109927; CCTTTATTCG-
Amplicon BHQ-1 Location: 71845-71919 RPA3 7p22 AGCGCGATT TTTCTCGAC
6FAM- GenBank GCGATTTAGG ACCAATCAA TCCAACTTCG Number CGAA
CCAATTAAAT AC004948; ACGCGAAA- Amplicon BHQ-1 Location: 23978-24056
RUNX3 1p36 CGTTCGATGG GACGAACAA 6FAM- GenBank TGGACGTGT CGTCTTATT
CGCACGAACT Number ACAACGC CGCCTACGTA AL023096; ATCCG-BHQ-1 Amplicon
Location: 64646-64762 S100A2 1q21 TGTTTGAGTC CGTATCATT 6FAM-
Muller, H. M. GTAAGTAGG ACAATACCG ATCCTCCCTT et al. Cancer GCGT
ACCTCCT TCTTATCCGC Lett209, 231- CAAACCCT- 236 (2004) BHQ-1 SASH1
6q23 TGGAAGAGTT GCGACTCGT 6FAM- GenBank TATTTTGAAG TCCTTCTAA
AAACCCGACA Number AGAGGG CAAATC AAAATAACCG AL513164; CGAAACCT-
Amplicon BHQ-1 Location: 97419-97530 SCAM-1 8p21 GTTTCGGTTG
ACGCCGAC 6FAM- GenBank TCGTTGGGTT GAACTCTAC ACGACGCAAT Number GC
CAAAACCCGC AC037459; GA-BHQ-1 Amplicon Location: 86568-86640
SCGB3A1 5q35- GGCGTAGCG CTACGTAAC 6FAM- GenBank qter GGCGTC
CCTATCCTA CGAACTCCTA Number CAACTCCG ACGCGCACG AC122714; ATAAAACCTA
Amplicon A-BHQ-1 Location: 80825-80911 SERPINB5 18q21.3 GAAAAGGAAT
ATAAACCAC 6FAM- GenBank AGGTAAGCG CGCTACTTC CACGATCGC Number AGGAGT
TACCCA CTCCACATCC AC036176; AAATCTTT- Amplicon BHQ-1 Location:
51709-51788 SEZ6L 22q12.1 GCGTTAGTAG ATACCAACC 6FAM- GenBank
GGAGAGAAA GCCTCCTCT CCGTCGACC Number ACGTTC AACC CTACAAAATT
AL022337; TAACGCCA- Amplicon BHQ-1 Location: 87324-87426 SFN 1p35.3
GAGGAGGGT ATCGCACAC 6FAM- GenBank TCGGAGGAG GCCCTAAAA TCTCCCGATA
Number AA CT CTCACGCACC AF029081; TCGAA-BHQ-1 Amplicon Location:
8848-8928 SFRP1 8p12- GAATTCGTTC AAACGAACC 6FAM- GenBank p11.1
GCGAGGGA GCACTCGTT CCGTCACCG Number ACC ACGCGAAAAC AC104393;
CAAT-BHQ-1 Amplicon Location: 1133-1202 SFRP2 4q31.3 GCGTTTTAGT
AAACGACCG 6FAM- GenBank CGTCGGTTGT AAATTCGAA CGAACCCGC Number TAGT
CTTATC TCTCTTCGCT AC020703; AAATACGA- Amplicon BHQ-1 Location:
71046-71137 SFRP4 7p14- GTTGTTCGGG GCGAAACTC 6FAM- GenBank p13
CGGGTTC CGCCGTCTA AAACACGAAC Number AACGCCAACT AC018634; CTCAACCT-
Amplicon BHQ-1 Location: 76448-76526 SFRP5 10q24.1 GCGTTTGTAG
GAACCGCTA 6FAM- GenBank TTTATCGTGT CACGACCGC CGCCGCAATA Number
GGTAGA T CCTTAACATC AL358938; CCTACCG- Amplicon BHQ-1 Location:
45204-45296 SLC6A20 3p21.3 AGGCGAATAC TAAAACGAC 6FAM- GenBank
GAATTGTAGC GCGCCTAAC CCGCGCACT Number G G AAAACTACCG AJ289880;
TACCGAA- Amplicon BHQ-1 Location: 85407-85541 SMAD2 18q21.1
CGAGGCGGT CGCATTAAA 6FAM- GenBank AGGTTTTTAT ACGATTCCC CCGATCCCTC
Number AGGT GAT GCCAACGTC AC120349; GTAA-BHQ-1 Amplicon Location:
27348-27425 SMAD3 15q22- CGTGAAGCG TTAACCGCC 6FAM- Previously 15q23
TTTGTTGGGT TTCTCGCAC TCCTCCTACC described as C CGTTCTACTC MADH3 in
GCCCTTCTT- Ehrlich, M. et BHQ-1 al. Oncogene 21, 6694- 6702 (2002)
SMAD4 18q21.1 GTTTGCGTAG GCAACTTTC 6FAM- GenBank AGCGATTTTT
CTTTCTCCC CCCGCCTCC Number TTC GACT CGCTCCGAAT AB043547; A-BHQ-1
Amplicon Location: 119423- 119492 SMAD6 15q21.3- ATGTTAGTTT
CGACCCTAC 6FAM- GenBank 22.2 AGATATTTTG AATAAAACG AAACCTTATT Number
GCGGTTTC TATTCTCCT TACGCAACAA AC013564; TCAACGCCG- Amplicon BHQ-1
Location: 57206-57309 SMAD9 13q12- CGCGAAGTTT CGAAAACGA 6FAM-
GenBank q14 TATCGTTCGT ACCGCAAAC AACTCCCTAA Number ATTAG A
CCGCTTTCCA AL138706; AATCGACG- Amplicon BHQ-1 Location: 77288-77362
SMUG1 12q13.11- GGATTATAGG TCACACCCG 6FAM- GenBank q13.3 CGCGCGTTAT
TAATCCGAA ACCGAAACGA Number T CA ACGAATCACG AC023794; AAATCAAA-
Amplicon BHQ-1 Location: 156022- 156167 SOCS1 16p13.13 GCGTCGAGTT
CCGAAACCA 6FAM- Fiegl, H. et al CGTGGGTATT TCTTCACGC ACAATTCCGC
Cancer T TAA TAACGACTAT Epidemiol CGCGCA- Biomarkers BHQ-1 Prev 13,
882- 888 (2004) STAT1 2q32.2 GCGTAGGATT AACAAACCC 6FAM- GenBank
CGGAAGGGT CAAACCGAA AACGACCCAA Number TA CA CGCGCTCGA AY865620;
AAA-BHQ-1 Amplicon Location: 2091-2179
STK11 19p13.3 AATTAACGGG GCCATCTTA 6FAM- GenBank TGGGTACGTC
TTTACCTCC CGCACGCCC Number G CTCCC GACCGCAA- AC011544; BHQ-1
Amplicon Location: 26084-26187 SYK 9q22 AGGGTCGTT AACATAAAC 6FAM-
GenBank GGGTGTTTGT CGCATCGAT CGCCAACGC Number G CCC GATAACTTCT
AL354862; ATAACTACCC Amplicon AA-BHQ-1 Location: 50529-50613 TERT
5p15.33 GGATTCGCG CGAAATCCG 6FAM- Fiegl, H. et al GGTATAGACG
CGCGAAA CCCAATCCCT Cancer TT CCGCCACGT Epidemiol AAAA-BHQ-1
Biomarkers Prev 13, 882- 888 (2004) TFAP2A 6p24 CGTTAATTTT
CCGACAACC 6FAM- GenBank TAAAGTATTT AACACTTTA CGAAACCGAA Number
TTATGGATCG CGC AAAAACATAT AL138885; CCGTTCACG- Amplicon BHQ-1
Location: 105985- 106093 TFF1 21q22.3 TAAGGTTACG ACCTTAATC 6FAM-
Fiegl, H. et al GTGGTTATTT CAAATCCTA CCCTCCCGC Cancer CGTGA
CTCATATCT CAAAATAAAT Epidemiol AAAA ACTATACTCA Biomarkers CTACAAAA-
Prev 13, 882- BHQ-1 888 (2004) TGFBR1 9q22 ACGCGCGTTT ACGAACCCG
6FAM- GenBank ATTGGTTGTC CAAACGAAA TAAATCCCGC Number TTAACAACTC
AL162427; GCGACGA- Amplicon BHQ-1 Location: 88267-88365 TGFBR2 3p22
GCGCGGAGC CAAACCCCG 6FAM- Eads, C. A. et GTAGTTAGG CTACTCGTC
CACGAACGA al. Cancer AT CGCCTTCCC Res 61, GAA-BHQ-1 3410-3418
(2001) THBS1 15q15 CGACGCACC GTTTTGAGT 6FAM- Widschwendter,
AACCTACCG TGGTTTTAC ACGCCGCGC M. et al GTTCGTT TCACCTCCCT- Cancer
Res BHQ-1 64, 3807- 3813 (2004) THRB 3p24.3 TCGTCGTCGT GCGTCTACG
6FAM- GenBank TATCGTCGC AACCGATAA CCCTCCAACC Number CCTAAT
CTCACGACTA AC012087; TCCGACTTA- Amplicon BHQ-1 Location: 123758-
123833 TIMP3 22q12.3 GCGTCGGAG CTCTCCAAA 6FAM- Eads, C.A. et
GTTAAGGTTG ATTACCGTA AACTCGCTCG al. Cancer TT CGCG CCCGCCGAA- Res
61, BHQ-1 3410-3418 (2001) TITF1 14q13 CGAAATAAAC TGTTTTGTT 6FAM-
Fiegl, H. et al CGAATCCTCC GTTTTAGCG CTCGCGTTTA Cancer TTAA TTTACGT
TTTTAACCCG Epidemiol ACGCCA- Biomarkers BHQ-1 Prev 13, 882- 888
(2004) TMEFF2 2q32.3 CGACGAGGA CAACGCCTA 6FAM- GenBank GGTGTAAGG
ACGAACGAA TATAACTTCC Number ATG CC GCGACCGCC AC092644; TCCTCCT-
Amplicon BHQ-1 Location: 149017- 149089 TNFRSF10A 8p21 AGTTTTTGGT
CAAACCCCG 6FAM- GenBank ATTTAGTAGG CAATAACCT ATTCCGCCAC Number
CGTTCG CTATATC CCATCCGTCC AC100861; A-BHQ-1 Amplicon Location:
53847-53923 TNFRSF10B 8p22- TTTTGGCGGT CTCATTTCC 6FAM- GenBank p21
TGCGTTTC CCCAAATTT ATCCTAACGC Number CGAT GAACAAAACC AC107959;
CAAAAACAA- Amplicon BHQ-1 Location: 131919- 132001 TNFRSF10C 8p22-
GGGAAGAGC TCCCCTAAC 6FAM- GenBank p21 GTATTTGGCG TCCGACGAC
CGAACATACC Number G CGACCGCAA AC107959; ATAACCA- Amplicon BHQ-1
Location: 165904- 166026 TNFRSF10D 8p21 GGGAAGAGC TCCCCTAAC 6FAM-
GenBank GTATTTGGCG TCCGACGAC TACCCGACC Number G GCAAACGAC AC100861;
CCG-BHQ-1 Amplicon Location: 115508- 115632 TNFRSF25 1p36.2
GCGGAATTAC ACTCCATAA 6FAM- Formerly GACGGGTAG CCCTCCGAC CGCCCAAAAA
described as A GA CTTCCCGACT TNFRSF12 CCGTA-BHQ-1 in Ehrlich, M. et
al. Oncogene 21, 6694- 6702 (2002) TP53 17p13.1 TTTGTTGTCG
CGAATTCCG 6FAM- GenBank CGGGATTTC TAAATCGCC TAATCCGAAA Number C
TACGACGACC AC087388; CAATCGAAAA- Amplicon 3'BHQ Location:
66667-66749 TP73 1p36.3 GGGTCGGGT CGATTTCGC 6FAM- GenBank
AGTTCGTTTT TACGTCCCC AACCTCCGAA Number G T CGAATACGC AF235000;
GAACGAA- Amplicon BHQ-1 Location: 3977-4058 TSHR.sup.d 14q31
TTGAGGGTTA ACAACGAAA 6FAM- GenBank GAGGCGGGT ATCCTCCTC AACGACGACT
Number A CAAAAATAC TCGACCGCA AC010072; A CCG-BHQ-1 Amplicon
Location: 103024- 103110 TWIST1 7p21.2 GTAGCGCGG AAACGCAAC 6FAM-
Muller, H. M. CGAACGT GAATCATAA CCAACGCAC et al. Cancer CCAAC
CCAATCGCTA Lett209, 231- AACGA-BHQ-1 236 (2004) TYMS 18p11.32
CGGCGTTAG TCTCAAACT 6FAM- Eads, C. A. et GAAGGACGA ATAACGCGC
CCGAATACCG al. Cancer T CTACAT ACAAAATACC Res 61, GATACCCGT-
3410-3418 BHQ-1 (2001) UNG 12q23- GTTTGACGGA ACAACGACG 6FAM-
GenBank q24.1 GGGCGTGTA ACTATTTTAA CCCGAATTTA Number ACACGTAA
CCGAATCAAA AC007637; AACGCGA- Amplicon BHQ-1 Location: 4765-4860
UQCRH 1p33.1 TTCGGTTTCG CCCATATAA 6FAM- GenBank GGTTTTAACG
ACGCTCACC CCCGCACAA Number GC CTCGAACAAA AL122001; ACGAAA-BHQ-
Amplicon 1 Location: 120731- 120802 VDR 12q12- ACGTATTTGG CGCTTCAAC
6FAM- Muller, H. M. q14 TTTAGGCGTT CTATATTAAT CCCACCCTTC et al.
Cancer CGTA CGAAAATAC CTACCGTAAT Lett209, 231- A TCTACCCAA- 236
(2004) BHQ-1 VHL 3p26- CGGGAGCGC CTCCGAAAC 6FAM- GenBank p25
GTACGTAGTT ATTCCCTCC CGAACCGAA Number G CGCCGCGAA AF010238; A-BHQ-1
Amplicon Location: 632-725 XAB2 19p13.2 GACGGATAG CGCATCTTC 6FAM-
GenBank GTTTACGTTA TAACGCCTC ACTTCCGATC Number TTGATTTT TATTC
GCTAACGTCG AC008763; TCGAAA-BHQ- Amplicon Location: 60446-60523 XPA
9q22.3 CGCGGAGTT CAACATCAA 6FAM- GenBank GTTTGTTTCG TACCCGCTA
CCGCTCGATA Number CCG CTCGCCCGC AL445531; A-BHQ-1 Amplicon
Location: 26708-26771 XPC 3p25.3 GTCGGGTGC CTACGCAAT 6FAM- GenBank
GTTATTCGC TCGCGTCCC ACCGCGCGT Number TTCCGAACCA AC093495; TATTACT-
Amplicon BHQ-1 Location: 81528-81625 XRCC1 19g13.2 CGTTGTTAAG
GCGCGAAAC 6FAM- GenBank GAACGTAGC TCGAACCTT CCAATCGCG Number GTTTT
T CCTCTCCAAA L34079; ACG-BHQ-1 Amplicon Location: 4045-4154 COL2A1
12g13.11- TCTAACAATT GGGAAGATG 6FAM- Widschwendter, q13.2
ATAAACTCCA GGATAGAAG CCTTCATTCT M. et al ACCACCAA GGAATAT
AACCCAATAC Cancer Res CTATCCCACC 64, 3807- TCTAAA-BHQ- 3813 (2004)
1 ALU N/A GGTTAGGTAT ATTAACTAA 6FAM- Weisenberger, AGTGGTTTAT
ACTAATCTT CCTACCTTAA D. J. et al ATTTGTAATTT AAACTCCTA CCTCCC-
Nucleic Acids TAGTA ACCTCA MGBNFQ Res 33, 6823-6836 (2005).
TABLE-US-00004 TABLE 2 (supplemental table 2). New CIMP
Classification Panel. The first five reactions are
methylation-specific, bisulfite conversion-specific markers that
together form the new diagnostic panel used for CIMP
classification. Putative DNA methylation sites (CpGs indicated as
"CG" in the sequence) are indicated in bold in the oligonucleotide
sequences. The sixth reactions is a non-methylation-specific,
bisulfite-conversion-specific normalization reactions used to
calculate PMR values. HUGO NAME RXN ID PURPOSE FORWARD PRIMER
REVERSE PRIMER PROBE CACNA1G HB-158 CIMP Marker TTTTTTCGTTTCGCGTTTA
CTCGAAACGACTTCGCCG 6FAM- GGT AAATAACGCCGAATCCGACAA CCGA-BHQ IGF2
HB-319 CIMP Marker GAGCGGTTTCGGTGTCGT CCAACTCGATTTAAACCGAC 6FAM- TA
G CCCTCTACCGTCGCGAACCCG A-BHQ NEUROG1 HB-261 CIMP Marker
CGTGTAGCGTTCGGGTAT CGATAATTACGAACACACTCC 6FAM- TTGTA GAAT
CGATAACGACCTCCCGCGAAC ATAAA-BHQ-1 RUNX3 HB-181 CIMP Marker
CGTTCGATGGTGGACGT GACGAACAACGTCTTATTACA 6FAM- GT ACGC
CGCACGAACTCGCCTACGTAA TCCG-BHQ-1 SOCS1 HB-042 CIMP Marker
GCGTCGAGTTCGTGGGT CCGAAACCATCTTCACGCTAA 6FAM- ATTT
ACAATTCCGCTAACGACTATCG CGCA-BHQ-1 ALU HB-313 Normalization
GGTTAGGTATAGTGGTTT ATTAACTAAACTAATCTTAAA 6FAM-CCTACCTTAACCTCCC-
Control ATATTTGTAATTTTAGTA CTCCTAACCTCA MGBNFQ
Example 2
Cancer-Specific DNA Methylation Markers were Selected
[0280] The initial (original) definition of CIMP was based on
concordant methylation of Type C loci, and specifically excluded
markers that showed evidence of age-associated methylation in
normal tissues, referred to as "Type A" loci.sup.1. Therefore,
applicants performed a first screen of all 195 unique
MethyLight.TM. markers available in applicants' laboratory against
ten colorectal normal-tumor pairs (TABLE 1; under Methods above) to
eliminate markers that did not show evidence of tumor-associated
methylation. To avoid bias either for or against markers associated
with CIMP, five tumors previously characterized as CIMP+ (see
Methods above; "Tissue Samples") were used, and five CIMP- tumors,
and only those markers that failed to show tumor-specific
methylation in either of the two groups of tumors (FIG. 1) were
eliminated. It is important to note that this initial filter did
not introduce a bias for or against CIMP. It merely eliminated the
103 markers that would not be informative in subsequent analyses
since they are not tumor-specifically methylated (see Methods above
"Selection of Type C Markers" and FIG. 1).
[0281] Three criteria were used to select markers for further
evaluation. The excluded markers represent the 105 markers shown at
the top, while included markers refer to the lower 92 markers in
FIG. 2. First, any marker, for which the highest PMR value among
these 20 samples was not at least 2 was excluded. Second, any
marker for which the mean PMR for tumor samples was at least twice
that of normal samples was included. Third, any marker for which
all normal samples had a PMR<2, and for which all tumor samples
had a PMR>2 was included. Both the second and third criteria
were applied separately to both the CIMP+ tumors and CIMP- tumors,
and to all ten cases collectively. A marker was included if it
passed at least one of the two criteria in any of the three sample
comparisons. This resulted in a collection of 92 reactions that
passed this first relaxed screen for tumor-specificity. Among these
92 reactions were five methylation markers (CDKN2A (p16), MLH1,
MINT1, MINT2, and MINT31) that have been commonly used to define
CIMP status.
Example 3
CIMP-Specific DNA Methylation Markers were Selected
[0282] Applicants next determined the methylation status of the
remaining 92 tumor-specific markers in 48 independent colorectal
cancer cases, and conducted a hierarchical two-dimensional
unsupervised clustering of the data in order to assess if distinct
subsets of methylation behavior among groups of tumors would emerge
(FIG. 2). A well-defined subgroup of tumors is evident on the
right-hand side of the dendrogram. Other cluster analyses, such as
an index of the number of methylated loci, a Gaussian mixture
model, and partitioning around medioids (PAM).sup.7,8 all yielded
similar results (see Methods). Although applicants argue above that
the initial screen that gave rise to the 92 markers used in this
cluster analysis was unbiased with respect to CIMP, applicants
sought additional evidence to support this contention by repeating
the initial screen in FIG. 1 using only CIMP- tumors, and then
repeating the cluster analysis using the resulting 50 markers. This
analysis identified the same clusters as the entire subset of 92
markers. Therefore, tumor-specific markers that were selected using
only CIMP- tumors were able to correctly resolve the distinct
cluster, indicating that the CIMP cluster is not an anomaly caused
by a bias introduced by the inclusion of CIMP+ tumors in the
initial filter. Since many of the classic CIMP markers, such as
CDKN2A (p16), MINT1, MINT2, MINT31, and MLH1.sup.1,2 are more
frequently methylated in the distinct subset of tumors on the right
side of the dendrogram, applicants conclude that this cluster is
similar to the CIMP+ grouping originally identified on the basis of
bimodal distribution of methylation frequency by Toyota et
al..sup.1,2.
[0283] It is interesting to note that a different subset of CpG
islands located near the center of the tumor heatmap in FIG. 2
appears to be more frequently methylated in tumor samples that do
not belong to the CIMP cluster, but nevertheless show increased
methylation in tumor samples compared to their matched normal
mucosal counterparts. Inclusion of such markers in panels used to
define CIMP could reduce the classification power of the panel, and
possibly even lead one to conclude that CpG island hypermethylation
is a continuous trait in colorectal cancer and that CIMP does not
exist as a distinct feature.sup.3.
[0284] Since CIMP+ tumors form a distinct subgroup of colorectal
tumors, it will be important to accurately identify these tumors
without having to perform cluster analysis, so that the etiology
and clinical correlates of CIMP can be investigated. In order to
select the best markers that could be used to identify CIMP+
tumors, applicants used the cluster routines described above to
classify tumors as either CIMP+ or CIMP-. Applicants then applied
four different selection algorithms to these classifications to
find the markers that best identify CIMP for each algorithm (see
Methods above "CIMP Marker Selection"). This resulted in nine top
CIMP- predicting markers, which were selected for further study.
Applicants also retained the five best additional Type-C markers as
a precaution, since the CIMP marker selection described above was
driven by a relatively small number of CIMP+ tumors.
Example 4
Assembly and Evaluation of CIMP Panels
[0285] To obtain further insight into the relative performance of
these 14 markers in the classification of CIMP+ tumors, applicants
analyzed a third independent set of 187 tumors using these markers,
and observed a strongly bimodal distribution of tumors by number of
methylated markers (FIG. 3). To select the best possible
five-marker panel from these 14 markers, applicants evaluated all
2,002 possible five-marker panels by determining each panel's
bimodality, which is an intrinsic trait of each panel and does not
rely on prior CIMP definitions (see Methods above; "New CIMP
Classification Panel"). Applicants also considered the
MethyLight.TM. reaction performance characteristics of each
individual marker (see Methods above; "New CIMP Classification
Panel"), since the robustness of the assay can significantly impact
successful implementation in studies using challenging
paraffin-embedded, formalin-fixed tissues. The five-marker panel
that best satisfied the reaction performance criteria, and retained
a high ranking (86.sup.th percentile) for bimodality among the
2,002 possible five-marker panels, consisted of CACNA1G, IGF2 (a
non-imprinted island at this locus), NEUROG1, RUNX3, and SOCS1
(TABLE 2 (supplemental table 2) above).
[0286] Applicants used three methods to compare this new candidate
diagnostic panel to a classic CIMP panel consisting of five
traditional CIMP markers (CDKN2A (p16), MINT1, MINT2, MINT31, and
MLH1) (FIG. 4). First, applicants determined that 99% of the 2,002
five-marker panel combinations described above showed a better
bimodal distribution than the classic CIMP panel, whereas our new
panel was among the top 14% of panels. Second, applicants compared
the cross-panel classification error rates among various panels
compiled from the 14 markers analyzed in FIG. 3 plus the five
traditional CIMP markers that comprise the classic panel shown in
FIG. 4 (TABLE 3 (supplemental table 3) below).
TABLE-US-00005 TABLE 3 (SUPPLEMENTAL TABLE 3) Cross-panel
classification error rates among various CIMP classification
panels, expressed as percentages. Panel-14 Panel- (w/o Panel-14 19
Classic) (w/o New) Panel-9 Classic New Panel-19 0 1.1 2.1 3.7 13.4
2.7 (19 CIMP Genes) Panel-14 1.1 0 2.1 3.7 14.4 2.7 (w/o Classic)
Panel-14 2.1 2.1 0 2.7 13.4 4.8 (w/o New) Panel-9 3.7 3.7 2.7 0
16.0 6.4 (w/o New & w/o Classic) Classic Panel 13.4 14.4 13.4
16.0 0 15.0 New Panel 2.7 2.7 4.8 6.4 15.0 0 For each panel, the
threshold distinguishing CIMP+ from CIMP- samples was chosen by
minimizing the within group sum of squared errors. For the panels
of 5 markers, samples containing at least 3 methylated markers were
considered CIMP+, 4 methylated markers for panels of 9, 7
methylated markers for panels of 14, and 9 methylated markers for
the panel of 19. If all panels are capturing the same information,
then one would expect to find relatively low cross-panel
classification error rates. The new panel of five markers
outperforms the panel of classic CIMP loci in every comparison. It
even gives lower classification error than the classic panel
against a panel of 14 markers that includes the classic panel, but
excludes the new panel. This suggests that the new panel captures
the group concordance better than the classic panel.
[0287] The new panel of five markers outperformed the panel of
classic CIMP loci in every comparison. The third strategy that
applicants used to evaluate the performance of panels was to
compare their associations with characteristics of colorectal
cancer that have previously been reported to be associated with
CIMP+ status. It is assumed that if this association reflects an
important underlying biological relationship, then a superior CIMP
classification would result in a stronger association. The
relationships between the methylation behavior of the two panels
and BRAF mutation, KRAS mutation, MSI status, HNPCC status, sex,
and proximal colonic location are represented graphically in FIG.
4, while the statistical analyses using the new CIMP panel
classification are summarized in TABLE 4.
TABLE-US-00006 TABLE 4 Distribution of covariates by New CIMP
Panel. CIMP+ CIMP- OVERALL (3-5 loci) (0-2 loci) VARIABLE N % N % N
% P-value TOTAL 187 100% 33 18% 154 82% SEX Male 103 55% 13 39% 90
58% Female 84 45% 20 61% 64 42% 0.05 SUBSITE Proximal 57 33% 19 59%
38 27% Distal 118 67% 13 41% 105 73% 0.0005 No Info 12 MSI MSI-high
21 11% 12 36% 9 6% STATUS MSI-low 19 10% 4 12% 15 10% MSS 147 79%
17 52% 130 84% 3.1.sup.-5 HNPCC Yes 8 4% 0 0% 8 5% STATUS No 179
96% 33 100% 146 95% 0.35 MLH1 Yes 16 9% 13 39% 3 2% METHYLATION No
171 91% 20 61% 151 98% 2.6.sup.-9 BRAF Mutant 26 14% 24 73% 2 1%
MUTATION WT 161 86% 9 27% 152 99% 1.6.sup.-21 KRAS Mutant 55 31% 3
10% 52 35% MUTATION WT 123 69% 28 90% 95 65% .002 No Info 9 MEAN
AGE (SD)* 65.5 68.6 64.8 0.13 (12.9) (12.7) (12.9) P-values are for
likelihood ratio tests from logistic regression with CIMP status as
the outcome. For the variable HNPCC we report the p-value from
Fisher's exact test. *Four CIMP- subjects are missing age.
[0288] Of particular note is the extremely strong relationship
between CIMP and BRAF mutation (P=1.6.times.10.sup.-21). The Odds
Ratio for this association is 203 (95% CI 41, 995), a far stronger
association of CIMP and BRAF mutation than any reported so far in
the literature.sup.5,9, and is a further indication of the
classification accuracy of applicants' new five-marker panel.
[0289] Applicants' tight definition of CIMP appears to exclude
tumors with KRAS mutation, which has been reported by others to be
associated with CIMP.sup.5,10. To investigate this further,
applicants determined the KRAS mutation status of the tumors
clustered in FIG. 2. KRAS mutant tumors are distributed across the
dendrogram, but show some minor clusters, which appear to be less
homogeneous with respect to their methylation profile than the
major CIMP cluster (FIG. 2). Interestingly, in this analysis, all
of the CIMP+ tumors, with one exception, have either a BRAF or KRAS
mutation.
[0290] To further investigate the association between KRAS or BRAF
mutation status and methylation behavior, applicants investigated
the individual associations of the 14 markers shown in FIG. 3, plus
the five classic CIMP markers TABLE 4 (supplemental table 4).
[0291] All 19 markers were signficantly associated with BRAF
mutation. Three markers (CDKN2A, CRABP1 and NEUROG1) were
positively associated with KRAS mutation after exclusion of BRAF
mutant tumors, indicating that a separate KRAS-associated CIMP
subgrouping exists with an overlapping set of methylation markers.
The biological significance of the very tight association between
the major CIMP and BRAF mutation is not clear, but it is
interesting to note that transformation of fibroblasts by fos or
ras oncogenes involves upregulation of DNA methyltransferase DNMT1
expression and increased global DNA methylation.sup.11,12.
[0292] Applicants find that microsatellite instability (MSI-H),
with the exception of one tumor, is fully explained by either a
confirmed HNPCC association or CIMP+ associated MLH1 methylation
(FIG. 4). Since MLH1 methylation-associated microsatellite
instability generally does not occur among sporadic cases outside
the context of CIMP, it appears that the underlying basis for
mismatch repair deficiency in sporadic colorectal cancer is a
broader epigenetic control defect that affects MLH1 in some but not
all CIMP tumors. Therefore, an accurate classification of CIMP+
tumors will be essential to understanding the etiology of sporadic
MSI-H colorectal tumors. CIMP and BRAF mutation are highly
associated with the serrated pathway of colorectal cancer
development.sup.9, and therefore an epigenetic regulatory defect
may be implicated in the genesis of serrated neoplasia. Dissecting
the separate clinical and etiological features associated with
mismatch repair deficiency, CIMP, proximal tumor location, and BRAF
mutation status will further elucidate this mechanism.
[0293] Since the panel of tumors shown in FIGS. 3 and 4 contributed
to the development of applicants' new CIMP diagnostic panel, we
evaluated the new CIMP panel and the classic panel on a fourth set
of independent tumors (FIG. 5). This analysis confirmed that the
new panel outperforms the classic panel and easily recognizes a
distinct, heavily methylated subset of colorectal tumors that
encompasses almost all BRAF mutant, and sporadic MSI-H colorectal
tumors.
[0294] In summary, applicants have provided definitive evidence for
the existence of CIMP as a distinct trait among colorectal
adenocarcinomas. Additionally, applicants have developed an
improved method for the classification of CIMP (See TABLE 5 for
representative preferred markers). Furthermore, applicants have
found that CIMP underlies almost all cases of sporadic MSI-H
colorectal cancer and tumors with mutation of the BRAF
oncogene.
TABLE-US-00007 TABLE 4 (SUPPLEMENTAL TABLE 4) Methylation frequency
by KRAS and BRAF status. KRAS-/ KRAS+/ KRAS-/ Overall BRAF- BRAF-
Fisher's BRAF+ Fisher's (N = 187) (N = 98) (N = 55) exact (N = 25)
exact Heterogeneity Reaction N % N % N % test p* N % test p$ test
p# BCL2.M1B..HB.140. 34 18% 11 11% 3 5% 0.381 18 72% 4.5E-09
5.6E-11 BDNF.M2B..HB.258. 32 17% 11 11% 7 13% 0.798 13 52% 3.1E-05
4.4E-05 CACNA1G.M1B..HB.158. 39 21% 11 11% 5 9% 0.788 20 80%
5.2E-11 3.2E-12 CALCA.M1B..HB.166. 37 20% 10 10% 9 16% 0.311 16 64%
9.7E-08 1.9E-07 CRABP1.M1B..HB.197. 62 33% 15 15% 21 38% 0.003 23
92% 6.7E-13 1.1E-12 IGF2.M2B..HB.319. 48 26% 14 14% 10 18% 0.644 22
88% 4.9E-12 3.7E-12 KL.M1B..HB.175. 40 21% 11 11% 5 9% 0.788 21 84%
4.2E-12 2.5E-13 NR3C1.M1B..HB.067. 37 20% 5 5% 6 11% 0.204 25 100%
1.7E-21 7.8E-23 RUNX3.M1B..HB.181. 34 18% 3 3% 4 7% 0.252 24 96%
3.4E-21 5.7E-23 GATA3.M1B..HB.327. 74 40% 26 27% 22 40% 0.103 25
100% 3.0E-12 9.1E-12 HOXA1.M2B..HB.268. 69 37% 36 37% 15 27% 0.285
15 60% 4.2E-02 2.2E-02 NEUROG1.M1B..HB.261. 66 35% 17 17% 22 40%
0.003 24 96% 1.5E-13 3.6E-13 SOCS1.M1B..HB.042. 28 15% 11 11% 2 4%
0.137 13 52% 3.1E-05 8.0E-07 DLEC1.M1B..HB.225. 28 15% 9 9% 3 5%
0.539 14 56% 1.5E-06 1.9E-07 MINT31.M1B..HB.162. 82 44% 29 30% 24
44% 0.111 25 100% 2.0E-11 6.7E-11 MINT1.M1B..HB.161. 77 41% 41 42%
15 27% 0.082 17 68% 2.5E-02 2.9E-03 MINT2.M1B..HB.187. 141 75% 68
69% 43 78% 0.264 24 96% 4.4E-03 1.1E-02 CDKN2A.M2B..HB.081. 55 29%
13 13% 20 36% 0.002 20 80% 3.1E-10 4.4E-10 MLH1.M2B..HB.150. 16 9%
3 3% 0 0% 0.553 12 48% 1.2E-07 7.3E-10 *comparing proportion
KRAS+/BRAF- to KRAS-/BRAF- $comparing proportion KRAS-/BRAF+ to
KRAS-/BRAF- #using Fisher's exact test
TABLE-US-00008 TABLE 5 Representative preferred markers. HUGO RXN
NAME RXN ID PURPOSE FORWARD PRIMER REVERSE PRIMER PROBE 1 CACNA1G
HB-158 CIMP Marker TTTTTTCGTTTCGCGTTT CTCGAAACGACTTCGCCG 6FAM- AGGT
AAATAACGCCGAATCCGACAA CCGA-BHQ 2 IGF2 HB-319 CIMP Marker
GAGCGGTTTCGGTGTCGTTA CCAACTCGATTTAAACCGACG 6FAM-
CCCTCTACCGTCGCGAACCCG A-BHQ 3 NEUROG1 HB-261 CIMP Marker
CGTGTAGCGTTCGGGTATT CGATAATTACGAACACACTCC 6FAM- TGTA GAAT
CGATAACGACCTCCCGCGAAC ATAAA-BHQ-1 4 RUNX3 HB-181 CIMP Marker
CGTTCGATGGTGGACGTGT GACGAACAACGTCTTATTA 6FAM- CAACGC
CGCACGAACTCGCCTACGTAA TCCG-BHQ-1 5 SOCS1 HB-042 CIMP Marker
GCGTCGAGTTCGTGGGTAT CCGAAACCATCTTCACGCTAA 6FAM- TT
ACAATTCCGCTAACGACTATCG CGCA-BHQ-1 6 ALU HB-313 Normalization
GGTTAGGTATAGTGGTTTA ATTAACTAAACTAATCTTA 6FAM-CCTACCTTAACCTCCC-
Control TATTTGTAATTTTAGTA AACTCCTAACCTCA MGBNFQ 7 COL2A1 HB-057
Normalization TCTAACAATTATAAACTCC GGGAAGATGGGATAGAAGGGA 6FAM-
Control AACCACCAA ATAT CCTTCATTCTAACCCAATACC TATCCCACCTCTAAA-BHQ-1
8 COL2A1 HD-005 Bisulfite GAAGATGGGATAGAAGGGA CTCCAACCACCAAACCTTC
6FAM- Conversion ATACATCT ATT GGCCCAATGCCTGTCCCACCT- Control
BHQ-1
Example 5
Use of the Colon Cooperative Family Registry (CFR) for a
Population-Based Study of CIMP
[0295] Further aspects of the present invention provide a
foundation for a population-based study of CIMP, by providing a
novel panel of very carefully selected methylation markers
representing the CIMP subgroup, and having utility to classify
CIMP.
[0296] Specifically, as described herein above, particular aspects
of the present invention provide novel methods and compositions for
determining the relationship between CIMP status and other
molecular features of the cancers including, but not limited to
BRAF mutation, KRAS mutation and MSI status.
[0297] Additional aspects provide novel methods and compositions
for determining the relationship between CIMP status and other
variables including, but not limited to age, sex, tumor location,
family history, race, country of origin, tumor characteristics
(including, tumor type, tumor grade, invasive margin
characteristics, lymphocyte infiltration characteristics, direct
spread, lymph node spread, venous spread and type of residual
adjacent polyp, if present).
[0298] Yet additional aspects provide novel methods and
compositions for determining, between subgroups defined by CIMP
status and BRAF mutations, effects of selected risk factors
including, but not limited to body mass index, smoking history,
alcohol intake, dietary folate intake, folate metabolic enzyme
polymorphisms and history of hormonal use.
Overview of the Colon Cooperative Family Registry (CFR)
[0299] The Cooperative Family Registry for Colorectal Cancer
Studies, referred to herein (and as known in the art) as the "Colon
CFR", is an NCl-supported consortium initiated in 1997 through a
competitive RFA. This consortium is dedicated to the establishment
of a comprehensive collaborative infrastructure for
interdisciplinary studies in the genetics and genetic epidemiology
of colorectal cancer. The cooperating institutions are collecting
epidemiological information and laboratory specimens from families
who represent the continuum of risk for CRC. Families are recruited
through the participating Registry sites, and as of July, 2002, the
CFR registry included data and biospecimens from approximately
7,773 probands plus selected close relatives, both affected and
unaffected, and, in some centers, additional unrelated
controls.
[0300] The CFR is an international consortium of six research
institutions and an Informatics Center. The participating centers
are at the: Fred Hutchinson Cancer Research Center, Seattle, Wash.
(P.I.: John Potter); Mayo Clinic, Rochester, Minn. (P.I.: Noralane
Lindor); University of Southern California, Los Angeles (P.I.:
Robert Haile); University of Queensland, Brisbane, Australia (P.I.:
John Hopper); Cancer Care Ontario, Ontario, Canada (P.I.: Steve
Gallinger); and the University of Hawaii Cancer Research Center,
Honolulu, Hawaii (P.I.: LoIc Le Marchand). The Informatics Center
(IC) is located at the University of California, Irvine (P.I.: Hoda
Anton Culver).
[0301] The six Registry centers use standardized instruments and
protocols to collect family history information, epidemiological
and clinical data, screening behavior, and related biological
specimens (blood samples and tumor blocks), with a strong emphasis
on quality control (QC) a and privacy measures throughout the
collection, processing, and storage of data and samples (see below
for key components). The CFR investigators have substantially
enriched the resource beyond the scope of the original RFA by
obtaining two collaborative supplements, one to characterize
colorectal tumors for microsatellite instability (MSI), and the
other to establish lymphoblastoid cell lines for particularly
informative registry participants.
[0302] The Colon CFR also developed a relevant core questionnaire
that is administered to all CFR participants, including cases,
controls, and affected and unaffected relatives. This standardized
instrument contains core questions asked by all centers, covering
established and suspected risk factors for colorectal cancer,
including medical history and medication use, reproductive history
(for female participants), family history, physical activity,
demographics, alcohol and tobacco use, and limited dietary factors.
Centers are allowed to customize questions for local usage, in
particular for different language usage and brand names, and to add
additional questions of interest. The method of administration of
the questionnaire is allowed to vary by center and has been
administered by face-to-face interview, over the telephone using a
Computer-Assisted Telephone Interview (CATI), or by mail for
self-administration. Detailed question-by-question manuals for
administration were developed for further standardization.
[0303] In addition to the risk factor questionnaire, which includes
a section on consumption of selected food items, all centers except
FHCRC and Mayo also administered a detailed food frequency
questionnaire. Australia used a version developed specifically for
Australia. The other centers used a well validated food frequency
questionnaire (FFQ) developed at the Cancer Research Center of
Hawaii (CRCH) (Stram, 2000). Expertise in dietary assessment has
been a strength of the Hawaii group for many years. The CRCH's
Nutrition Support Shared Resource consists of faculty
nutritionists, dietitians and computer programmers and includes an
extensive food composition database to accommodate the diets of
several US ethnic groups. This database contains more than 3,200
food items and more than 100 components (nutrients and
non-nutrients) and is continuously updated with information from
standard sources (such as the USDA), other sources (such as
international organizations), local recipes, and special food
analyses. This resource was used to develop a food composition
table for the CFR FFQ which is the same as the one used in the
Multiethnic Cohort Study.
[0304] The different sites of the CFR are collaborating on creating
a virtual data-analysis group to take advantage of the expertise
across the collaborating centers and to avoid duplication of
effort. The group will develop data-analysis strategies that are
suitable for the Colon CFR study population, will divide
development and analytic tasks according to specific expertise,
will oversee analysis for Colon CFR studies, and will contribute to
the final interpretation and write-up of findings. The data
analysis group includes Drs. Duncan Thomas (USC consortium), Kim
Siegmund (USC consortium, and co-Investigator on this application),
John Hopper (Australasian CFR), and Li Hsu (FHCRC). Drs. Hopper and
Thomas serve as co-directors of this unit. They report on their
activities to the Epidemiology and Analysis Working Group.
[0305] The University of Melbourne Consortium (J. Hopper, Ph.D., P.
I.) recruits clinic-based families from family cancer clinics and
population-based families from cancer registries and electoral
rolls. During Phase I (1997-2002) 250 clinic-based families were
recruited in Brisbane, Melbourne, Adelaide, Sydney, and Perth,
Australia, and in Auckland, New Zealand. Clinic-based families
included those with HNPCC or HNPCC-like family histories. In
addition, 627 population-based CRC cases selected from the
Victorian Cancer Registry in Melbourne, aged between 18 and 59
years at diagnosis, and their first- and second-degree relatives
have been recruited. Also, 260 non-cancer population-based controls
selected through the electoral roll and their first- and
second-degree relatives have been recruited. Epidemiology/family
history questionnaires were completed on all respondents by either
in-person or telephone interview. Tumor blocks and blood samples
have been collected on approximately 800 cases. Blood samples were
collected from approximately 3,800 cases, controls and their
relatives. There were 1,351 population-based probands selected and
eligible. Of these, 141 were deceased and the physician refused
permission to contact 85, leaving 1,125 (83%) that they were
allowed to contact. Of these, they were unable to contact 174,
leaving 951 probands who were selected and eligible. Of these 951,
324 (34%) refused and the remaining 627 (66%) joined the CFR. There
were 1545 siblings of these 627 probands. They had permission to
contact 840. Only 2 of these 840 refused to participate. Of the 705
siblings whom they had no permission to contact, they have proxy
questionnaires completed by a participating relative for 675.
[0306] University of Hawaii Cancer Research Center (P.I.: Loic Le
Marchand, U01 CA74806): The Hawaii CFR site has focused on
recruiting multi-case CRC families in the multiethnic population of
Hawaii. This approach was originally selected in order to increase
the study's efficiency by enriching the sample for genetic risk
factors. All new incident CRC cases diagnosed in the state of
Hawaii in 1997-2001 (n=1,800) were screened through personal
interview for family history. Those with a positive history of CRC
among first-degree relatives were invited to participate in the
CFR, along with their first-degree relatives. In addition, 60
multi-case families identified in a recently completed
population-based, case-control family study of CRC were invited to
participate in the Registry. These families were prospectively
ascertained through contact with all CRC incident cases under age
60 years diagnosed between 1987 and 1996 among Japanese, Caucasian,
Hawaiian and Chinese residents of Oahu (where 85% of the population
of the state reside). Pedigrees have been expanded to include
second-degree relatives for some large multiplex families.
Epidemiology and family history questionnaires were obtained from
950 participants. In addition, 155 tumor blocks and 767 blood
samples were collected. For probands, there were 2,245 selected and
eligible cases. Of these, they received permission to contact 1,988
(89%). Of these 1988, they were unable to contact 41, leaving 1,947
cases. Of these 1,947 cases, 140 refused, 57 were deceased, and
they lost contact with 54, so 1,696 (87%) completed a family
history screening questionnaire. Of these 1,696, 1,397 were not
eligible for the CFR by virtue of their family history and 51 were
not selected for further recruitment, leaving 248 eligible and
selected for recruitment. Of these 248, 64 refused, 8 were
deceased, and they lost contact with 1, leaving 175 (70%) who
participated in the CFR. The total number of siblings was 879, of
whom 852 were eligible. Of these siblings, 439 (52%)
participated.
[0307] The Fred Hutchinson Cancer Research Center (J. Potter, M.
D., Ph.D., P. I.) identifies colorectal cancer (CRC) cases through
the population-based Puget Sound SEER (NCI) Program. During Phase I
(1997-2002), all incident CRC cases diagnosed between the ages of
20 and 74 in three Washington state counties and their first-degree
relatives were ascertained. Age and gender-matched population-based
controls were ascertained from two population lists--controls under
age 65 years were recruited through driver's license lists;
controls 65-74 years were selected from Health Care Finance
Administration (HCFA, currently CMS) files.
[0308] Epidemiology/family history questionnaires were completed on
all respondents over the telephone using a computer-assisted
telephone interview (CATI) format. Tumor blocks were collected on
all cases and CRC-affected family members (diagnosed less than 5
years prior to recruitment). Blood samples were collected from all
cases, all family members in high-risk families, a sample of family
members in non-high-risk families, and a sample of controls. During
Phase I, 1,831 cases, 4,325 family members, and 1,531
population-based controls were enrolled. In addition, 1,570 tumor
blocks and 2,713 blood samples were collected. For probands,
initially there were 2,959 eligible. Of these, they received
permission to contact 2, 733 (92%) Of these 2,733, they were unable
to contact 22 and 364 were determined to be ineligible, leaving
2,347 selected and eligible. Of these 2,347, 267 refused, 185 were
deceased, and they lost contact with 64, so 1,831 (78%)
participated in the CFR. There were 2,225 siblings who were
eligible and selected. Of these 2,225, 173 refused, 7 were
deceased, and they lost contact with 66, so 1,979 siblings (89%)
participated in the CFR.
[0309] The Mayo Clinic (N. M. Lindor, M.D., Principal Investigator)
recruited 479 probands from high or intermediate risk families from
the population-based Minnesota Cancer Surveillance System (MCSS),
selected from 4,471 incident cases diagnosed over a four-year
period from 1997 through 2000. Cases were surveyed for family
history and high/intermediate risk families were defined as those
with two or more cases of CRC, those with known familial
adenomatous polyposis (FAP), those in which the proband was
diagnosed under the age of 50 years, and families reporting an
hereditary non-polyposis colon cancer-like (HNPCC-like) tumor
spectrum. In addition, a random sampling of respondents from the
MCSS was conducted until 215 randomly selected participants were
identified and recruited. An additional 245 high- or
intermediate-risk families were also accrued from two
non-population-based sources: other Mayo Clinic Rochester patients
and the North Central Cancer Treatment Group. Recruitment of
high-risk families was extended to parents, affected and unaffected
siblings (up to four, aiming for same sex, oldest sibs); if a
parent was affected, aunts and uncles on that side of the family
were also invited to enroll. Overall, 2,469 relatives of CRC
probands were enrolled (provided informed consent, blood specimens,
medical record access, and completed the epidemiology risk factor
questionnaire). Unaffected relatives of probands can serve as
controls. Spouses of individuals with cancer were also recruited as
another type of control. Tumor blocks from 633 CRC participants
(probands and affected relatives) and 161 non-CRC were collected
and tested for microsatellite instability and immunohistochemistry
for the main DNA mismatch repair gene proteins. For the
population-based probands, there were initially 5,002 potentially
eligible cases. Of these, the physician refused contact for 115 and
428 were deceased, so they had permission to contact 4,459. Of
these 4,459, 748 refused, 83 were deceased, and they lost contact
with 1,776, so 1,852 (42%) completed the screening questionnaire.
Of these 1,852 cases, 8 were deemed ineligible and 1,308 were not
selected for further recruitment, leaving 536 selected and
eligible. Of these 536 cases, 52 refused and 2 were deceased, so
482 (90%) participated in the CFR. They invited 844 siblings to
participate in the CFR. Of these 844, 572 (68%) agreed to
participate.
[0310] Cancer Care Ontario (S. Gallinger, M.D., P. I.): The Cancer
Care Ontario site is a consortium that includes eight Regional
Cancer Centers covering the entire province of Ontario. During
Phase I, 8,818 incident CRC cases, diagnosed between 1997-2000,
were identified from the population-based Ontario Cancer Registry.
Family histories were obtained on 3,780 cases and stratified by
risk (high=meets Amsterdam criteria for HNPCC;
intermediate=HNPCC-like and/or other specific features such as
young age, multiple polyps; low=none of the above). Recruitment (at
least one core element) was successful for 70 high-risk families,
845 intermediate-risk families and 430 low-risk families (which is
a 25% random sample of this larger latter group). In addition,
2,679 first- and second-degree relatives of registered probands
were recruited, and 890 non-cancer, age and gender-matched
population controls using residential telephone lists and 1,021
population controls using the Ontario ministry of finance property
assessment file for year 2000 were also recruited. Blood samples
have been obtained for 1,052 probands, 2,080 relatives, 1,289
controls, and 1,372 tumors (mostly CRC specimens from probands)
have been processed. For probands, there were 8,726 eligible cases.
Of these, they received permission to contact 7,229 (83%). Of these
7,229, 24 were deemed ineligible and they were unable to contact
164, leaving 7,041 who were eligible and selected. Of these 7,041
cases, 2,960 refused and 301 were deceased, leaving 3,780 (54%) who
completed the screening questionnaire. Of these 3,780 cases, 1 was
ineligible and 1,929 were not selected for further recruitment
based on their family history, leaving 1,850 selected and eligible.
Of these 1,850 cases, 217 refused, 27 were deceased, and they lost
contact with 7, so 1,599 (86%) participated in the CFR. They
invited 1565 siblings to join the CFR and 837 (53.5%) of these
siblings participated.
[0311] The University of Southern California Consortium (R. Haile,
DrPH., P. I.) includes the Universities of Southern California,
North Carolina, Colorado, Arizona, and Minnesota, Dartmouth Medical
School, and the Cleveland Clinic. From the population based cancer
registries of all but the last of these sub-centers, this group
contacted 33% of CRC cases diagnosed in Whites over a one and a
half-year period. In addition, sixty-six percent of CRC cases in
African-American, Asian, and Hispanic families plus all CRC cases
diagnosed under age 50 years over the preceding three years were
contacted. These cases were screened for a family history of CRC.
Sixteen percent of single-case families and all multiple-case
families were invited to participate in the CFR. A total of 633
families from these population-based sources were recruited into
the Registry. The majority (about 60%) are from multiple-case
families and a substantial proportion are from racial minorities.
These are supplemented by 120 HNPCC (Amsterdam criteria positive),
or HNPCC-like families from the Cleveland Clinic. USC serves as the
coordinating center and Dartmouth the data center for this
consortium. Unaffected relatives of probands are selected as
controls. For the population-based probands, there were initially
5,684 potentially eligible cases. The physician refused permission
to contact 103 cases, so they had permission to contact 5,581 (98%)
of these cases. Of these 5,581, they were unable to contact 191 and
656 were deemed ineligible, leaving 4,734 eligible for a screening
questionnaire. Of these 4,734,413 refused, 927 were deceased, and
they lost contact with 291, so 3,103 (66%) completed the family
history screening questionnaire. Of these 3,103,105 were
ineligible, and 1,943 were not selected for further recruitment
based on their family history, leaving 1,055 selected and eligible.
Of these 1,055 cases, 325 refused, 75 were deceased, and they lost
contact with 22, so 633 participated in the CFR. There were 746
siblings. Of these 746, 96 were excluded (deceased, cognitively
impaired, language barrier and other reasons). Of the remaining
650, 247 refused and they lost contact with 14, so 389 (60%)
siblings participated in the CFR.
[0312] Informatics Center (H. Anton-Culver, Ph.D., P.I.). The NCI
has designated the University of California Irvine's Epidemiology
Division as the Informatics Center to support the CFR. The
Informatics Center: [0313] The CFR Informatics Center (IC) has
designed and maintained a secure, data processing, storage, and
retrieval system that contains the core Cancer Family Registries
data with the flexibility to integrate data generated through
additional studies into the core schema structure; [0314] The IC
has provided guidelines for data standardization, established
methods for data transmission, developed computerized validation
checks, monitors quality of data, and prepares data for reporting;
[0315] Provides statistical and study design support for the CFR
investigators and generates reports for special requests and
descriptive summary statistics; [0316] Helps facilitate CFR-wide
communication, coordination, and administration; [0317] Developed
and maintains a secure web site to provide relevant information to
CFR members; [0318] Developed web-based software tools allowing CFR
members to query database for family characteristics, view
pedigrees, download files, and create tables and charts using data
from the CFR database housed at the IC; and [0319] Developed
web-based software to enter, process, and tracking investigator
applications to use CFR data and includes automated notifications
to management when new applications are received, as well as
notifications to applicants, SC and AC members and others regarding
the steps in the application process.
TABLE-US-00009 [0319] 47675-189 Sequence Listing Table Grouping SEQ
ID NOs Forward/Reverse Primers (14 Select) 1-28 Forward/Reverse
Primers (other genes) 29-67 Probes (14 Select) 68-81 Probes (other
genes) 82-99 Amplicons (14 Select) 100-113 CpG Islands (14 Select)
114-127 Genomic DNA (14 Select) 128-141 CpG Bisulfite Up (14
Select) 142-169 Genomic Bisulfite Up (14 Select) 170-197 CpG
Bisulfite Down (14 Select) 198-225 Genomic Bisulfite Down (14
Select) 226-253 Gene Coding Sequences (14 Select) 254, 256, 258,
260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284,
286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310,
312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334 Protein
Sequences (14 Select) 255, 257, 259, 261, 263, 265, 267, 269, 271,
273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295, 297,
299, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 323,
325, 327, 329, 331, 333, 335 SEQ ID Gene Sequence Type NO BCL2
Forward Primer 1 BCL2 Reverse Primer 2 BDNF Forward Primer 3 BDNF
Reverse Primer 4 CACNA1G Forward Primer 5 CACNA1G Reverse Primer 6
CALCA Forward Primer 7 CALCA Reverse Primer 8 CRABP1 Forward Primer
9 CRABP1 Reverse Primer 10 DLEC1 Forward Primer 11 DLEC1 Reverse
Primer 12 GATA3 Forward Primer 13 GATA3 Reverse Primer 14 HOXA1
Forward Primer 15 HOXA1 Reverse Primer 16 IGF2 Forward Primer 17
IGF2 Reverse Primer 18 KL Forward Primer 19 KL Reverse Primer 20
NEUROG1 Forward Primer 21 NEUROG1 Reverse Primer 22 NR3C1 Forward
Primer 23 NR3C1 Reverse Primer 24 RUNX3 Forward Primer 25 RUNX3
Reverse Primer 26 SOCS1 Forward Primer 27 SOCS1 Reverse Primer 28
CDH13 Forward Primer 29 CDH13 Reverse Primer 30 CDKN2A Forward
Primer 31 CDKN2A Reverse Primer 32 GATA4 Forward Primer 33 GATA4
Reverse Primer 34 GDNF Forward Primer 35 GDNF Reverse Primer 36
ITGA4 Forward Primer 37 ITGA4 Reverse Primer 38 MINT1 Forward
Primer 39 MINT1 Reverse Primer 40 MINT2 Forward Primer 41 MINT2
Reverse Primer 42 MINT31 Forward Primer 43 MINT31 Reverse Primer 44
MLH1 Forward Primer 45 MLH1 Reverse Primer 46 NEUROD2 Forward
Primer 47 NEUROD2 Reverse Primer 48 OPCML Forward Primer 49 OPCML
Reverse Primer 50 PENK Forward Primer 51 PENK Reverse Primer 52
SCGB3A1 Forward Primer 53 SCGB3A1 Reverse Primer 54 SFRP2 Forward
Primer 55 SFRP2 Reverse Primer 56 TFAP2A Forward Primer 57 TFAP2A
Reverse Primer 58 ALU Forward Primer 59 ALU Reverse Primer 60 BRAF
Primer 1 61 BRAF Primer 2 62 BRAF Primar 3 63 COL2A1 Forward Primer
64 COL2A1 Reverse Primer 65 COL2A1 (2) Forward Primer 66 COL2A1 (2)
Reverse Primer 67 BCL2 Probe 68 BDNF Probe 69 CACNA1G Probe 70
CALCA Probe 71 CRABP1 Probe 72 DLEC1 Probe 73 GATA3 Probe 74 HOXA1
Probe 75 IGF2 Probe 76 KL Probe 77 NEUROG1 Probe 78 NR3C1 Probe 79
RUNX3 Probe 80 SOCS1 Probe 81 CDH13 Probe 82 CDKN2A Probe 83 GATA4
Probe 84 GDNF Probe 85 ITGA4 Probe 86 MINT1 Probe 87 MINT2 Probe 88
MINT3 Probe 89 MLH1 Probe 90 NEUROD2 Probe 91 OPCML Probe 92 PENK
Probe 93 SCGB3A1 Probe 94 SFRP2 Probe 95 TFAP2A Probe 96 ALU Probe
97 COL2A1 Probe 98 COL2A1 (2) Probe 99 BCL2 Amplicon 100 BDNF
Amplicon 101 CACNA1G Amplicon 102 CALCA Amplicon 103 CRABP1
Amplicon 104 DLEC1 Amplicon 105 GATA3 Amplicon 106 HOXA1 Amplicon
107 IGF2 Amplicon 108 KL Amplicon 109 NEUROG1 Amplicon 110 NR3C1
Amplicon 111 RUNX3 Amplicon 112 SOCS1 Amplicon 113 BCL2 CpG Island
114 BDNF CpG Island 115 CACNA1G CpG Island 116 CALCA CpG Island 117
CRABP1 CpG Island 118 DLEC1 CpG Island 119 GATA3 CpG Island 120
HOXA1 CpG Island 121 IGF2 CpG Island 122 KL CpG Island 123 NEUROG1
CpG Island 124 NR3C1 CpG Island 125 RUNX3 CpG Island 126 SOCS1 CpG
Island 127 BCL2 Genomic DNA 128 BDNF Genomic DNA 129 CACNA1G
Genomic DNA 130 CALCA Genomic DNA 131 CRABP1 Genomic DNA 132 DLEC1
Genomic DNA 133 GATA3 Genomic DNA 134 HOXA1 Genomic DNA 135 IGF2
Genomic DNA 136 KL Genomic DNA 137 NEUROG1 Genomic DNA 138 NR3C1
Genomic DNA 139 RUNX3 Genomic DNA 140 SOCS1 Genomic DNA 141 BCL2
CpG Island Bisulphite Up Sense 142 BCL2 CpG Island Bisulphite Up
Antisense 143 BDNF CpG Island Bisulphite Up Sense 144 BDNF CpG
Island Bisulphite Up Antisense 145 CACNA1G CpG Island Bisulphite Up
Sense 146 CACNA1G CpG Island Bisulphite Up Antisense 147 CALCA CpG
Island Bisulphite Up Sense 148 CALCA CpG Island Bisulphite Up
Antisense 149 CRABP1 CpG Island Bisulphite Up Sense 150 CRABP1 CpG
Island Bisulphite Up Antisense 151 DLEC1 CpG Island Bisulphite Up
Sense 152 DLEC1 CpG Island Bisulphite Up Antisense 153 GATA3 CpG
Island Bisulphite Up Sense 154 GATA3 CpG Island Bisulphite Up
Antisense 155 HOXA1 CpG Island Bisulphite Up Sense 156 HOXA1 CpG
Island Bisulphite Up Antisense 157 IGF2 CpG Island Bisulphite Up
Sense 158 IGF2 CpG Island Bisulphite Up Antisense 159 KL CpG Island
Bisulphite Up Sense 160 KL CpG Island Bisulphite Up Antisense 161
NEUROG1 CpG Island Bisulphite Up Sense 162 NEUROG1 CpG Island
Bisulphite Up Antisense 163 NR3C1 CpG Island Bisulphite Up Sense
164 NR3C1 CpG Island Bisulphite Up Antisense 165 RUNX3 CpG Island
Bisulphite Up Sense 166 RUNX3 CpG Island Bisulphite Up Antisense
167 SOCS1 CpG Island Bisulphite Up Sense 168 SOCS1 CpG Island
Bisulphite Up Antisense 169 BCL2 Genomic Bisulphite Up Sense 170
BCL2 Genomic Bisulphite Up Antisense 171 BDNF Genomic Bisulphite Up
Sense 172 BDNF Genomic Bisulphite Up Antisense 173 CACNA1G Genomic
Bisulphite Up Sense 174 CACNA1G Genomic Bisulphite Up Antisense 175
CALCA Genomic Bisulphite Up Sense 176 CALCA Genomic Bisulphite Up
Antisense 177 CRABP1 Genomic Bisulphite Up Sense 178 CRABP1 Genomic
Bisulphite Up Antisense 179 DLEC1 Genomic Bisulphite Up Sense 180
DLEC1 Genomic Bisulphite Up Antisense 181 GATA3 Genomic Bisulphite
Up Sense 182 GATA3 Genomic Bisulphite Up Antisense 183 HOXA1
Genomic Bisulphite Up Sense 184 HOXA1 Genomic Bisulphite Up
Antisense 185 IGF2 Genomic Bisulphite Up Sense 186 IGF2 Genomic
Bisulphite Up Antisense 187 KL Genomic Bisulphite Up Sense 188 KL
Genomic Bisulphite Up Antisense 189 NEUROG1 Genomic Bisulphite Up
Sense 190 NEUROG1 Genomic Bisulphite Up Antisense 191 NR3C1 Genomic
Bisulphite Up Sense 192 NR3C1 Genomic Bisulphite Up Antisense 193
RUNX3 Genomic Bisulphite Up Sense 194 RUNX3 Genomic Bisulphite Up
Antisense 195 SOCS1 Genomic Bisulphite Up Sense 196 SOCS1 Genomic
Bisulphite Up Antisense 197 BCL2 CpG Island Bisulphite Down Sense
198 BCL2 CpG Island Bisulphite Down Antisense 199 BDNF CpG Island
Bisulphite Down Sense 200 BDNF CpG Island Bisulphite Down Antisense
201 CACNA1G CpG Island Bisulphite Down Sense 202 CACNA1G CpG Island
Bisulphite Down Antisense 203 CALCA CpG Island Bisulphite Down
Sense 207 CALCA CpG Island Bisulphite Down Antisense 205 CRABP1 CpG
Island Bisulphite Down Sense 206 CRABP1 CpG Island Bisulphite Down
Antisense 207 DLEC1 CpG Island Bisulphite Down Sense 208 DLEC1 CpG
Island Bisulphite Down Antisense 209 GATA3 CpG Island Bisulphite
Down Sense 210 GATA3 CpG Island Bisulphite Down Antisense 211 HOXA1
CpG Island Bisulphite Down Sense 212 HOXA1 CpG Island Bisulphite
Down Antisense 213
IGF2 CpG Island Bisulphite Down Sense 214 IGF2 CpG Island
Bisulphite Down Antisense 215 KL CpG Island Bisulphite Down Sense
216 KL CpG Island Bisulphite Down Antisense 217 NEUROG1 CpG Island
Bisulphite Down Sense 218 NEUROG1 CpG Island Bisulphite Down
Antisense 219 NR3C1 CpG Island Bisulphite Down Sense 220 NR3C1 CpG
Island Bisulphite Down Antisense 221 RUNX3 CpG Island Bisulphite
Down Sense 222 RUNX3 CpG Island Bisulphite Down Antisense 223 SOCS1
CpG Island Bisulphite Down Sense 224 SOCS1 CpG Island Bisulphite
Down Antisense 225 BCL2 Genomic Bisulphite Down Sense 226 BCL2
Genomic Bisulphite Down Antisense 227 BDNF Genomic Bisulphite Down
Sense 228 BDNF Genomic Bisulphite Down Antisense 229 CACNA1G
Genomic Bisulphite Down Sense 230 CACNA1G Genomic Bisulphite Down
Antisense 231 CALCA Genomic Bisulphite Down Sense 232 CALCA Genomic
Bisulphite Down Antisense 233 CRABP1 Genomic Bisulphite Down Sense
234 CRABP1 Genomic Bisulphite Down Antisense 235 DLEC1 Genomic
Bisulphite Down Sense 236 DLEC1 Genomic Bisulphite Down Antisense
237 GATA3 Genomic Bisulphite Down Sense 238 GATA3 Genomic
Bisulphite Down Antisense 239 HOXA1 Genomic Bisulphite Down Sense
240 HOXA1 Genomic Bisulphite Down Antisense 241 IGF2 Genomic
Bisulphite Down Sense 242 IGF2 Genomic Bisulphite Down Antisense
243 KL Genomic Bisulphite Down Sense 244 KL Genomic Bisulphite Down
Antisense 245 NEUROG1 Genomic Bisulphite Down Sense 246 NEUROG1
Genomic Bisulphite Down Antisense 247 NR3C1 Genomic Bisulphite Down
Sense 248 NR3C1 Genomic Bisulphite Down Antisense 249 RUNX3 Genomic
Bisulphite Down Sense 250 RUNX3 Genomic Bisulphite Down Antisense
251 SOCS1 Genomic Bisulphite Down Sense 252 SOCS1 Genomic
Bisulphite Down Antisense 253 BCL2 Alpha isoform mRNA 254 BCL2
Alpha isoform protein 255 BCL2 Beta isoform mRNA 256 BCL2 Beta
isoform protein 257 BDNF mRNA 258 BDNF Protein 259 CACNA1G mRNA
Sequence for alpha 1G subunit (isoform 1) 260 CACNA1G Protein
sequence for alpha 1G subunit (isoform 1) 261 CACNA1G mRNA Sequence
for alpha 1G subunit (isoform 2) 262 CACNA1G Protein Sequence for
alpha 1G subunit (isoform 2) 263 CACNA1G mRNA Sequence for alpha 1G
subunit (isoform 3) 264 CACNA1G Protein Sequence for alpha 1G
subunit (isoform 3) 265 CACNA1G mRNA Sequence for alpha 1G subunit
(isoform 4) 266 CACNA1G Protein Sequence for alpha 1G subunit
(isoform 4) 267 CACNA1G mRNA Sequence for alpha 1G subunit (isoform
5) 268 CACNA1G Protein Sequence for alpha 1G subunit (isoform 5)
269 CACNA1G mRNA Sequence for alpha 1G subunit (isoform 6) 270
CACNA1G Protein Sequence for alpha 1G subunit (isoform 6) 271
CACNA1G mRNA Sequence for alpha 1G subunit (isoform 7) 272 CACNA1G
Protein Sequence for alpha 1G subunit (isoform 7) 237 CACNA1G mRNA
Sequence for alpha 1G subunit (isoform 8) 274 CACNA1G Protein
Sequence for alpha 1G subunit (isoform 8) 275 CACNA1G mRNA Sequence
for alpha 1G subunit (isoform 9) 276 CACNA1G Protein Sequence for
alpha 1G subunit (isoform 9) 277 CACNA1G mRNA Sequence for alpha 1G
subunit (isoform 10) 278 CACNA1G Protein Sequence for alpha 1G
subunit (isoform 10) 279 CACNA1G mRNA Sequence for alpha 1G subunit
(isoform 11) 280 CACNA1G Protein Sequence for alpha 1G subunit
(isoform 11) 281 CACNA1G mRNA Sequence for alpha 1G subunit
(isoform 12) 282 CACNA1G Protein Sequence for alpha 1G subunit
(isoform 12) 283 CACNA1G mRNA Sequence for alpha 1G subunit
(isoform 13) 284 CACNA1G Protein Sequence for alpha 1G subunit
(isoform 13) 285 CACNA1G mRNA Sequence for alpha 1G subunit
(isoform 14) 286 CACNA1G Protein Sequence for alpha 1G subunit
(isoform 14) 287 CACNA1G mRNA Sequence for alpha 1G subunit
(isoform 15) 288 CACNA1G Protein Sequence for alpha 1G subunit
(isoform 15) 289 CALCA Transcript variant 1 mRNA 290 CALCA
Transcript variant 1 protein 291 CALCA Transcript variant 2 mRNA
292 CALCA Transcript variant 2 protein 293 CALCA Transcript variant
3 mRNA 294 CALCA Transcript variant 3 protein 295 CRABP1 mRNA 296
CRABP1 protein 297 DLEC1 Transcript variant DLEC1-N1 mRNA 298 DLEC1
Transcript variant DLEC1-N1 protein 299 GATA3 Isoform 1 mRNA 300
GATA3 Isoform 1 protein 301 GATA3 Isoform 2 mRNA 302 GATA3 Isoform
2 protein 303 HOXA1 Transcript variant 1 mRNA 304 HOXA1 Transcript
variant 1 protein 305 HOXA1 Transcript variant 2 mRNA 306 HOXA1
Transcript variant 2 protein 307 IGF2 mRNA 308 IGF2 protein 309 KL
Transcript variant 1 mRNA 310 KL Transcript variant 1 protein 311
KL Transcript variant 2 mRNA 312 KL Transcript variant 2 protein
313 NEUROG1 mRNA 314 NEUROG1 protein 315 NR3C1 Transcript variant 1
mRNA 316 NR3C1 Transcript variant 1 protein 317 NR3C1 Transcript
variant 2 mRNA 318 NR3C1 Transcript variant 2 protein 319 NR3C1
Transcript variant 3 mRNA 320 NR3C1 Transcript variant 3 protein
321 NR3C1 Transcript variant 4 mRNA 322 NR3C1 Transcript variant 4
protein 323 NR3C1 Transcript variant 5 mRNA 324 NR3C1 Transcript
variant 5 protein 325 NR3C1 Transcript variant 6 mRNA 326 NR3C1
Transcript variant 6 protein 327 NR3C1 Transcript variant 7 mRNA
328 NR3C1 Transcript variant 7 protein 329 RUNX1 Isoform 1 mRNA 330
RUNX1 Isoform 1 protein 331 RUNX1 Isoform 2 mRNA 332 RUNX1 Isoform
2 protein 333 SOCS1 mRNA 334 SOCS1 protein 335
TABLE-US-00010 Amplicon Table HUGO Gene Amplicon Amplicon Name
Amplicon Sequence Accession No. Location Length BCL2
CCGCATCCCGGGACCCGGTC Widschwendter, M. et 672-755 (in 82
GCCAGGACCTCGCCGCTGCA al Cancer Res 64, GenBank entry)
GACCCCGGCTGCCCCCGGCG 3807-3813 (2004); CCGCCGCGGGGCCTGCGCTC GenBank
Number AGCC NM00633 (SEQ ID NO: 100) BDNF CGCACCGGGCTGGCTCCTCT
GenBank Number 3794-3866 72 GTCCGGCCCGGGAGCCCGAG AC103796
GCGCTACGGGGTGCGCGGGA CAGCGAGCGGGCG (SEQ ID NO: 101) CACNA1G
CTTCTTCGCTTCGCGCCCAG GenBank Number 48345-48411 66
GCTCCGGTTGCCGGATTCGG ACO21491 CGCTACCTTCGGCGAAGCCG CCCCGAG (SEQ ID
NO: 102) CALCA GTTCTGGAAGCATGAGGGTG GenBank Number 1706-1806 100
ACGCAACCCAGGGGCAAAGG X15943 ACCCCTCCGCCCATTGGTTG
CTGTGCACTGGCGGAACTTT CCCGACCCACAGCGGCGGGA A (SEQ ID NO: 103) CRABP1
TCGAAATTCTCGCTGCTGCG GenBank Number 122142-122223 81
CATCTTCCAGGTGCCGGCGA AC011270 AGTTGGGCATGGTGGCGGTG
GCGGCGGCGGCAGGTACGGA CA (SEQ ID NO: 104) DLEC1 TCGCTGCGCACCCAAGATAT
GenBank Number 19959-20088 129 CTCGCACTTGCTCACCGGCG AP006309
TCTTCCGCAACTTGTACTCA GCCGAGGTCATCGGCGACGA AGTGAGCGCAAGCTTGATCA
AGGCCCGCGGCAGCGAGAAT GAGCGCCACG (SEQ ID NO: 105) GATA3
TGCACCGGGACGGAATCGTC GenBank Number 51880-51959 79
CACCCGACCCGAATGAATTG AL390294 GCAGGAGCCGCGGCCACATT
TAAAGGGCCAGAGCGCGCGT (SEQ ID NO: 106) HOXA1 CTGCCCACTAGGAAGCGGTC
GenBank Number 78138-78220 82 GTCGCCGCCGCAACTGTTGG AC004079
CGCTGACCGCGCACGACTGG AAAGTTGTAATCCTATGGTC CGA (SEQ ID NO: 107) IGF2
GAGCGGCCCCGGTGCCGCCA GenBank Number 108633-108720 87
CCGCCTGTCCCCCTCCCGAG M132217 GCCCGGGCTCGCGACGGCAG
AGGGCTCCGTCGGCCCAAAC CGAGCTGG (SEQ ID NO: 108) KL
AGCCTGGCTCCCGCGCAGCA GenBank Number 2062-2189 127
TGCCCGCCAGCGCCCCGCCG AB009667 CGCCGCCCGCGGCCGCCGCC
GCAGTCGCTGTCGCTGCTGC TGGTGCTGCTGGGCCTGGGC GGCCGCCGCCTGCGTGCGGA
GCCGGGCG (SEQ ID NO: 109) NEUROG1 CGTGCAGCGCCCGGGTATTT GenBank
Number 75342-75429 87 GCATAATTTATGCTCGCGGG AC005738
AGGCCGCCATCGCCCCTCCC CCAACCCGGAGTGTGCCCGT AATTACCG (SEQ ID NO: 110)
NR3C1 GGGTGGAAGGAGACGCCGCA GenBank Number 1786-1861 75
GCCGGAGCGGCCGAAGCAGC AY436590 TGGGACCGGGACGGGGCACG CGCGCCCGGAAGCCC
(SEQ ID NO: 111) RUNX3 GACGGGCAGCGTCTTGTTGC GenBank Number
64646-64762 116 AGCGCCAGTGCGAGGGCAGC AL023096 ACGGAGCAGAGGAAGTTGGG
GCTGTCGGTGCGCACGAGCT CGCCTGCGTGGTCCGCCAGC ACGTCCACCATCGAGCG (SEQ ID
NO: 112) SOCS1 CGAGCCCGTGGGCACCTTCC Fiegl, H. et al Cancer
2808-2887 79 TGGTGCGCGACAGCCGCCAG Epidemiol Biomarkers
CGGAACTGCTTTTTCGCCCT Prev 13,882-888 TAGCGTGAAGATGGCCTCGG (2004);
GenBank (SEQ ID NO: 113) Number DQ086801
Sequence CWU 0 SQTB SEQUENCE LISTING The patent application
contains a lengthy "Sequence Listing" section. A copy of the
"Sequence Listing" is available in electronic form from the USPTO
web site
(http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20120219946A1).
An electronic copy of the "Sequence Listing" will also be available
from the USPTO upon request and payment of the fee set forth in 37
CFR 1.19(b)(3).
0 SQTB SEQUENCE LISTING The patent application contains a lengthy
"Sequence Listing" section. A copy of the "Sequence Listing" is
available in electronic form from the USPTO web site
(http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20120219946A1).
An electronic copy of the "Sequence Listing" will also be available
from the USPTO upon request and payment of the fee set forth in 37
CFR 1.19(b)(3).
* * * * *
References