U.S. patent application number 12/746506 was filed with the patent office on 2011-12-22 for antimicrobial composition based on botanical extracts from oil palm vegetation liquor.
This patent application is currently assigned to Malaysian Palm Oil Board. Invention is credited to Ravigadevi Sambanthmurthi, Yew Ai Tan.
Application Number | 20110311656 12/746506 |
Document ID | / |
Family ID | 40718385 |
Filed Date | 2011-12-22 |
United States Patent
Application |
20110311656 |
Kind Code |
A1 |
Sambanthmurthi; Ravigadevi ;
et al. |
December 22, 2011 |
ANTIMICROBIAL COMPOSITION BASED ON BOTANICAL EXTRACTS FROM OIL PALM
VEGETATION LIQUOR
Abstract
The present invention provides an antimicrobial composition
comprising compounds extracted from the vegetation liquor of the
palm oil milling process has been suggested. This composition is
rich in antioxidants and exhibits enhanced antimicrobial activity
and bactericidal effect against a number of micro-organisms.
Inventors: |
Sambanthmurthi; Ravigadevi;
(Selangor Darul Ehsan, MY) ; Tan; Yew Ai; (Kuala
Lumpur, MY) |
Assignee: |
Malaysian Palm Oil Board
|
Family ID: |
40718385 |
Appl. No.: |
12/746506 |
Filed: |
December 5, 2008 |
PCT Filed: |
December 5, 2008 |
PCT NO: |
PCT/MY2008/000177 |
371 Date: |
August 20, 2010 |
Current U.S.
Class: |
424/727 |
Current CPC
Class: |
A61P 31/04 20180101;
A01N 65/00 20130101; A61P 39/06 20180101 |
Class at
Publication: |
424/727 |
International
Class: |
A61K 36/889 20060101
A61K036/889; A61P 39/06 20060101 A61P039/06; A61P 31/04 20060101
A61P031/04 |
Foreign Application Data
Date |
Code |
Application Number |
Dec 7, 2007 |
MY |
PI 20072189 |
Claims
1. An antimicrobial composition comprising compounds extracted from
the vegetation liquor of the palm oil milling process wherein said
composition slows or arrests the growth of one or more
microorganisms.
2. The antimicrobial composition of claim 1 wherein the composition
is rich in antioxidants.
3. The antimicrobial composition of claim 1 wherein said compounds
from the vegetation liquor of the palm oil milling process comprise
one or more compounds selected from the group consisting of
phenolic compounds, fruit acids, fruit sugars and glycerol.
4. The antimicrobial composition of claim 1 wherein said
composition exhibits an inhibitory effect against micro-organisms
as measured by the disc diffusion method of antimicrobial
susceptibility.
5. The antimicrobial composition of claim 1 wherein said
composition is bactericidal or bacteriostatic against gram positive
and/or gram negative bacteria.
6. (canceled)
7. The antimicrobial composition of claim 1 wherein said
composition is devoid of adverse drug reactions in a patient in
need thereof.
8. The antimicrobial composition of claim 1 wherein said
composition is in substantially pure form.
9. The antimicrobial composition of claim 1 wherein said
composition is incorporated in pharmaceutical formulations for
treatment of one or more diseases or infections.
10. (canceled)
11. The antimicrobial composition of claim 1 wherein said
composition further comprises additional components which do not
materially affect the properties of the composition.
12. A method for obtaining an antimicrobial composition comprising
extracting compounds from the vegetation liquor of the palm oil
milling process with a suitable solvent, wherein said composition
slows or arrests the growth of one or more microorganisms.
13. The method of claim 12 further comprising separating the
extract from the supernatant.
14. The method of claim 13 further comprising removing the solvent
from the extract.
15. The method of claim 12 wherein said compounds are extracted
from the vegetation liquor using one or more suitable solvents
selected from the group consisting of ethanol, methanol, acetone,
ethyl acetate and butanol.
16. The method of claim 12 wherein said compounds are enriched
using membrane filtration technology.
17. The method of claim 12 wherein said compounds from the
vegetation liquor of the palm oil milling process comprise one or
more compounds selected from the group consisting of phenolic
compounds, fruit acids, fruit sugars and glycerol.
18. The method of claim 12 wherein said composition is rich in
antioxidants.
19. The method of claim 12 wherein said composition is devoid of
adverse drug reactions in a patient in need thereof.
20. The method of claim 12 wherein said composition is in
substantially pure form.
21. The method of claim 12 wherein said composition further
comprises additional components which do not materially affect the
properties of the composition.
22. The method of claim 12 wherein said composition is incorporated
in pharmaceutical formulations for treatment of one or more
diseases or infections.
Description
FIELD OF INVENTION
[0001] The present invention relates to compositions having
antimicrobial properties and comprises compounds extracted from
plant material. More particularly, the present invention relates to
a composition comprising botanical extracts containing phenolic
compounds from vegetation liquor of oil palm for a wide scope of
antimicrobial applications.
BACKGROUND OF INVENTION
[0002] In the health industry of the emergent world, bacterial
related diseases and infections which are recognized or identified
are mostly therapeutically prevailed by way of antibiotics and
bacteriostatic agents. Accordingly, there is a great momentum in
producing modern microbial antibiotics and medicaments globally.
Nonetheless, such modern antibiotics and medicaments may represent
a considerable number of complications including prohibitively
expensive in terms of its sources and increasing toxic effects.
Owing to this eminent circumstance, substantial progress has been
made in this regard in order to further determine and thus provide
a natural origin solution to economically produce such antibiotics
or bacteriostatic based treatments.
[0003] Proceeding from the above, one of the natural origin
solutions is discovered in plants, whereby the usage of phenolics
and flavanoids, which are most abundant and ubiquitous products of
secondary plant metabolism, has been recently implicated
therapeutically. Under normal condition, they are often used as a
defense mechanism against animal predation and the like.
[0004] Plants which are used for medicinal studies contain a wide
range of substances and compounds that can be used to treat chronic
as well as infectious diseases. Such implication is increasing
rapidly, more particularly in areas where the use of plant based
medicines is of great importance.
[0005] Additionally, recent studies have begun to examine and
heightened the role of plants extract in the context of producing
treatments or medications against microbial diseases. Further
studies on the extracts and biologically active compounds isolated
from plants have significantly increased in the past decades.
[0006] A relatively conventional exemplary of a plant species which
is found to be exhibiting considerable antimicrobial effect is the
sesame leaves (Shittu 2006). Studies were conducted based on the
leaves of the said plant species (Sesame radiatum) for
antimicrobial activity, and the ethanolic extract of said plant has
shown mild inhibitory effect on the growth of streptococcus
pneumoniae and candida albicans. The said ethanolic extract
contained essential oils, mainly phenolic and carboxylic acids
groups.
[0007] The present invention focuses on the enhancement of the
medicinal properties of another prominent plant species, namely the
palm fruit (Elaies guineensis). It is presently known in the art
that the palm fruit botanical extract contains a rich source of
phenolic compounds, beta-carotene and vitamin E, which are
prominent nutritional antioxidants that act as scavengers of oxygen
atom or free radicals, and thus known to be effective against skin
aging, heart disease and cancer.
[0008] In addition, the palm fruit is the source of palm oil which
is obtained from palm fruit and palm kernel oil, whereby the source
is the fruit seeds. According to studies, palm oil and palm kernel
oil are composed of about 50% and 80% of fatty acids, respectively.
The palm fruit contains fatty acids ranging from C.sub.6 to
C.sub.18, at which the palm oil is found to contain high amount of
palmitic acid while the palm kernel oil is found to contain high
amount of lauric acid. In addition to the phenolic compounds,
Vitamin E and beta-carotene, the high content of fatty acids in
palm fruit may also play a significant role in providing the
bacteriostatic effect on certain and antimicrobial activity on
certain micro-organisms.
[0009] Apart from the above, high content of phenolics may
contribute significantly to bactericidal properties in palm fruit
based extract.
[0010] The present invention provides a new approach in using palm
fruit, particularly in preparing a composition based on botanical
extracts obtained from oil palm vegetation liquor for providing
bacteriostatic or inhibitory effect against a number of
micro-organisms.
[0011] It is further added that the high content of phenolics in
the composition in accordance with the present invention provides
bactericidal effect against micro-organisms.
[0012] Consequently, the primary object of the present invention to
provide a composition based on compounds extracted from vegetation
liquor of the palm oil milling process where the composition
exhibits inhibitory effect against micro-organisms.
[0013] It is a further object of the present invention to provide
an improved composition and method based on compounds extracted
from vegetation liquor of the palm oil milling process for
producing a broad spectrum antimicrobial effect which is
non-toxic.
[0014] It is further the object of the present invention to provide
an improved composition and formulation for producing a composition
containing antioxidants and phenolics obtained from the botanical
extracts of oil palm vegetation liquor devoid of chemically
prepared adverse drug reactions in a patient in need thereof.
[0015] It is a further object of the present invention to provide a
composition and formulation for producing a composition containing
compounds obtained from the botanical extracts oil palm vegetation
liquor having antimicrobial or bacteriostatic effect.
[0016] It is a further object of the present invention to provide a
composition and formulation for producing a composition containing
compounds obtained from the botanical extracts oil palm vegetation
liquor having bactericidal effect against micro-organisms due to
the high content of phenolics in the said composition.
SUMMARY OF INVENTION
[0017] The present invention discloses an antimicrobial composition
comprising compounds extracted from the vegetation liquor of the
palm oil milling process.
DETAILED DESCRIPTION OF THE INVENTION
[0018] Further understanding of the object, construction,
characteristics and functions of the invention, a detailed
description with reference to the embodiments is given in the
following.
[0019] The inventors of the present invention discovered that the
composition prepared from the extract of oil palm vegetation
exhibits enhanced antimicrobial activity.
[0020] It is therefore purpose of this invention is to present
composition containing extract of oil palm vegetation liquor from
the oil palm milling process for antimicrobial applications, in
particular for treatments of bacterial related diseases and
infections.
[0021] The composition of the present invention may prevent/inhibit
the growth of micro-organism(s). This may include slowing or
arresting a micro-organism, such as bacteria or by eliminating the
said micro-organism(s) present on contact with the composition of
the present invention.
[0022] It is noted that it would be advantageous for the
composition of the present invention to be bactericidal or
bacteriostatic effective against gram positive and gram negative
bacteria.
[0023] Accordingly, the present invention also relates to an
extraction process of antioxidants from the oil palm vegetation
liquor from the milling process. The processing of oil palm
produces larger amounts of vegetation liquor rich in phenolic
compounds, fruit acids, fruit sugars and glycerol which can be
further enriched using conventional membrane filtration
technology.
[0024] It is noted that extracts derived from the vegetation liquor
of oil palm, wherein the major constituents are phenolics, fruit
acids, fruit sugars and glycerol have properties that exhibit
antimicrobial activity and bactericidal effect against certain
micro-organisms.
BEST MODE FOR CARRYING OUT THE INVENTION
[0025] Materials and Methods
[0026] Extract Materials
[0027] The preparation of the composition of the present invention
is described in detail by referring to the experimental examples.
However, the present invention is not limited to these
examples.
[0028] Accordingly, the present invention also relates to an
extraction process of antioxidants from the oil palm vegetation
liquor from the milling process. The processing of oil palm
produces larger amounts of vegetation liquor rich in phenolic
compounds, fruit acids, fruit sugars and glycerol which can be
further enriched using conventional membrane filtration
technology.
[0029] For the experimental examples, inventors isolated botanical
extracts comprising phenolic compounds, fruit acids, fruit sugars
and glycerol from oil palm vegetation liquor from the palm oil
milling process and the prepared formulations containing these
extracts.
[0030] Preparation of Extracts
[0031] Unless otherwise specified, throughout the following
examples, the oil palm phenolic extract employed is a crude
solvent-free extract.
[0032] The present invention focuses on antimicrobial compositions
comprising of high content of phenolic compounds which is
discovered to exhibit antimicrobial and bactericidal
properties.
[0033] The biologically active extracts of palm vegetation liquor
useful in this invention can be prepared by any means capable of
extracting phenolic compounds from the vegetation liquor using
standard extraction techniques or techniques well known in the art.
Such extractions include but are not limited to ethanol, methanol,
acetone, ethyl acetate and butanol.
[0034] In addition to direct use of an extract, it is also possible
to use different fractions of the oil palm phenolic compounds. What
constitutes an effective amount of an extract, or an active portion
therof, will depend on the purity of the extract. For example, if a
crude phenolic-containing extract of about 10% purity is employed,
the extract will normally be used at a concentration range of about
0.01-20% by weight of the composition. At a higher level of purity,
a smaller percentage will be required to achieve the same effect.
Assuming a substantially pure phenolic extract, i.e. an extract
containing at least 80% active phenolics, the level will be about
0.01 to about 8% by weight of the composition.
[0035] Antimicrobial Screening
[0036] One of the conventional steps which may be employed in
regards to the antimicrobial screening methodology is agar-well
diffusion method of the modified Collins et. al (1995) whereby the
antimicrobial activity of the composition of the present invention
against a number of micro-organisms is accordingly tested.
[0037] It should be noted that other suitable conventional
methodologies may also be selected for the purpose of the
antimicrobial screening.
[0038] Antimicrobial Activity
[0039] The antimicrobial activity may be employed based on the
conventional disc or paper diffusion method. In the disc diffusion
method, different concentrations of extracts are loaded on the
sterile disc, and subsequently placed on the surface of the medium
as prepared. The plates are prepared with the suitable media,
poured into petri plates. Subsequently, the said plates are allowed
to solidify and a predetermined amount of innoculum suspension is
swabbed uniformly across the plates and allowed to dry within a
predetermined period. In the next step, the loaded disc accordingly
positioned on the surface of the medium and allowed to diffuse
within a predetermined period of time. The plates are therefore
kept for incubation at a suitable temperature and period of time.
Upon completion incubation, the inhibition zones are formed around
the disc and thus measured. The results indicate that the extract
performed inhibitory activity against the tested micro-organisms.
This experiment may be performed in triplicate.
[0040] It should be noted that other suitable conventional
methodologies may also be selected for the purpose of the
antimicrobial activity.
[0041] Application(s)
[0042] The antimicrobial composition in accordance with the present
invention may be used for a wide range of applications.
[0043] In one aspect it is understood by a person skill in the art
that the composition of the present invention may be used in pure
form or in combination with additional components which preferably
do not materially affect the properties of the composition.
[0044] In another aspect, the composition may be incorporated in
pharmaceutical formulations for treatment of one or more diseases
or infections.
[0045] In a further aspect, the composition may be used for
personal hygiene or cosmoceutical applications.
[0046] Although the present invention has been described with
reference to the preferred embodiments and examples thereof, it is
apparent to those skilled in the art that a variety of
modifications and changes may be made without departing from the
scope of the present invention which is intended to be defined by
the appended claims.
* * * * *