U.S. patent application number 13/138378 was filed with the patent office on 2011-11-24 for whitening agent, anti-aging agent, and anti-oxidant agent.
This patent application is currently assigned to Shiseido Company, Ltd.. Invention is credited to Kiyotaka Hasegawa, Ken Kusakari, Kiyoshi Sato, Rikako Suzuki, Tokiya Yokoi.
Application Number | 20110286953 13/138378 |
Document ID | / |
Family ID | 42541910 |
Filed Date | 2011-11-24 |
United States Patent
Application |
20110286953 |
Kind Code |
A1 |
Suzuki; Rikako ; et
al. |
November 24, 2011 |
WHITENING AGENT, ANTI-AGING AGENT, AND ANTI-OXIDANT AGENT
Abstract
[Problem] To provide a whitening agent having a superior skin
whitening activity, and having a superior effect in lightening and
whitening pigmentation, aging spots, freckles, chloasma, or the
like after sunburn, and an antioxidant agent and an anti-aging
agent having a superior free radical scavenging ability for
preventing and controlling aging in the skin and skin diseases
caused by active oxygen (free radicals), and having a high level of
safety. [Means for Solving the Problem] An extract of Psilotum
spp., the family Psilotaceae, the genus Psilotum is contained.
Inventors: |
Suzuki; Rikako; (Kanagawa,
JP) ; Hasegawa; Kiyotaka; (Kanagawa, JP) ;
Sato; Kiyoshi; (Kanagawa, JP) ; Yokoi; Tokiya;
(Kanagawa, JP) ; Kusakari; Ken; (Kanagawa,
JP) |
Assignee: |
Shiseido Company, Ltd.
|
Family ID: |
42541910 |
Appl. No.: |
13/138378 |
Filed: |
February 3, 2010 |
PCT Filed: |
February 3, 2010 |
PCT NO: |
PCT/JP2010/000623 |
371 Date: |
August 8, 2011 |
Current U.S.
Class: |
424/62 ;
424/725 |
Current CPC
Class: |
A61Q 19/02 20130101;
A61Q 19/08 20130101; A61K 8/9741 20170801; A61P 39/06 20180101;
A61P 17/18 20180101; A61K 36/11 20130101 |
Class at
Publication: |
424/62 ;
424/725 |
International
Class: |
A61K 8/97 20060101
A61K008/97; A61Q 19/08 20060101 A61Q019/08; A61Q 19/02 20060101
A61Q019/02; A61K 36/12 20060101 A61K036/12; A61Q 19/00 20060101
A61Q019/00 |
Foreign Application Data
Date |
Code |
Application Number |
Feb 9, 2009 |
JP |
2009-027227 |
Claims
1. A whitening agent characterized by comprising an extract of
Psilotum spp., the family Psilotaceae.
2. A whitening agent according to claim 1, characterized in that
the Psilotum spp. is Psilotum nudum.
3. A skin external preparation for whitening characterized by
comprising an extract of Psilotum spp., the family Psilotaceae.
4. An anti-aging agent characterized by comprising an extract of
Psilotum spp., the family Psilotaceae.
5. An anti-aging agent according to claim 4, characterized in that
the Psilotum spp. is Psilotum nudum.
6. A skin external preparation for anti-aging characterized by
comprising an extract of Psilotum spp., the family Psilotaceae.
7. An antioxidant agent characterized by comprising an extract of
Psilotum spp., the family Psilotaceae.
8. An antioxidant agent according to claim 7, characterized in that
the Psilotum spp., the family Psilotaceae is Psilotum nudum.
9. A skin external preparation for antioxidation characterized by
comprising an extract of Psilotum spp., the family Psilotaceae.
Description
TECHNICAL FIELD
[0001] The present invention relates to a whitening agent, an
anti-aging agent, and an antioxidant agent, more specifically,
relates to a whitening agent, an anti-aging agent, an antioxidant
agent comprising a plant extract as an active ingredient, and a
skin external preparation comprised thereof.
BACKGROUND ART
[0002] While the pathogenesis of aging spots, etc., is partially
unknown, it is generally considered that abnormalities of hormones
or the stimulation by ultraviolet rays from sunlight causes, the
formation of melanin pigments and the pigments are abnormally
deposited in the skin. The melanin pigments which cause
pigmentation of the skin are produced in melanin-producing granules
(melanosomes) within melanin cells (melanocytes) between the
epidermis and the hypodermis, and the produced melanin diffuses to
adjacent cells by osmosis.
[0003] The biochemical reaction in melanocytes is presumed to be as
follows. Namely, the production process of melanin pigment is that
tyrosine, an essential amino acid becomes dopaquinone by the action
of the enzyme, tyrosinase, and the dopaquinone is converted to
black melanin by enzymatic or non-enzymatic oxidation activity.
[0004] Various whitening agents for controlling the occurrence of
the aforementioned melanin pigments, comprising extracts derived
from plants have been conventionally developed in the anticipation
that such extracts are safe and mildly irritating to the skin
(refer to, for example, Patent Documents 1 to 4).
[0005] However, it is well known that active oxygen is generated by
ultraviolet rays. Among active oxygen species, free radical type
active oxygen reacts with an oxidizable substrate such as lipid,
inducing an oxidation chain reaction. Therefore, active oxygen
which can become free radicals amplifies damage to body tissue such
as skin.
[0006] The skin is always exposed to oxygen and ultraviolet rays,
and thus, is the tissue which has the greatest oxidative stress
damage by free radicals. Recently, it has been considered that a
variety of active oxygen species generated by ultraviolet rays
cause the peroxidation of sebum and lipid, protein degeneration,
enzyme inhibition, etc., and thereby, the inflammation, etc., of
the skin is induced over the short term, and aging, cancer, and the
like are caused over a prolonged period of time.
[0007] Further, it is considered that active oxygen and peroxidized
lipids are associated with skin diseases such as atopic dermatitis,
contact dermatitis, and psoriasis. In this way, active oxygen (free
radicals) is deeply involved in aging of the skin and skin
diseases.
[0008] Substances having the ability to scavenge free radicals can
control and terminate free radical chain reactions, and correspond
to, for example, substances referred to as antioxidant agents.
[0009] Therefore, skin external preparations comprising an
antioxidant agent are anticipated to have an effect of prevention
and improvement of aging in the skin (for example, aging spots,
wrinkles, sagging skin, etc.) caused by photooxidative stress.
Further, the skin external preparations can be anticipated to have
an effect of prevention and improvement, as skin external
preparations for various skin diseases associated with free
radicals.
[0010] Vitamin E and vitamin C which are known as antioxidant
agents are in vivo free radicals scavenger antioxidant substances.
Further, the synthetic antioxidant substances of BHT and BHA are
also known. Furthermore, as conventional antioxidant agents derived
from plants, extracts of Chinese mushrooms, enoki mushrooms,
shimeji mushrooms, maitake mushrooms, matsutake mushrooms,
Ganoderma lucidum, Daedalea dickinsii, Pholiota nameko, and other
basidiomycetes have been reported (Patent Documents 6 to 8).
Furthermore, antioxidant agents consisting of extracts of a plant
belonging to the genus Verbascum of the family Scrophulariaceae
(Patent Document 9) and antioxidant agents consisting of extracts
of a plant belonging to the genus Cordia of the family Boraginaceae
(Patent Document 10) have been reported.
[0011] Further, Patent Document 5 mentions a solvent extraction of
Psilotum nudum belonging to the family Psilotaceae as a plant
extract comprising apigenin or amentoflavone. Patent Document 5
shows no data that this plant extract has a melanogenesis
stimulation activity, thus, in Patent Document 5, the function of
the solvent extraction of Psilotum nudum itself is unclear. The
present invention relates to the melanogenesis-inhibitory action
and the free radical scavenging-type antioxidation activity due to
the Psilotum extract. Thus, the description of Patent Document 5
and the contents of the present invention are completely
different.
PRIOR ART DOCUMENTS
Patent Documents
[0012] [Patent Document 1] Japanese Unexamined Patent Publication
(Kokai) No. 8-310939 [0013] [Patent Document 2] Japanese Unexamined
Patent Publication (Kokai) No. 7-89843 [0014] [Patent Document 3]
Japanese Unexamined Patent Publication (Kokai) No. 9-30954 [0015]
[Patent Document 4] Japanese Unexamined Patent Publication (Kokai)
No. 2003-73224 [0016] [Patent Document 5] Japanese Unexamined
Patent Publication (Kokai) No. 9-263534 [0017] [Patent Document 6]
Japanese Unexamined Patent Publication (Kokai) No. 5-317016 [0018]
[Patent Document 7] Japanese Unexamined Patent Publication (Kokai)
No. 6-65575 [0019] [Patent Document 8] Japanese Unexamined Patent
Publication (Kokai) No. 59-124984 [0020] [Patent Document 9]
Japanese Unexamined Patent Publication (Kokai) No. 11-171723 [0021]
[Patent Document 10] Japanese Unexamined Patent Publication (Kokai)
No. 11-171720
DISCLOSURE OF THE INVENTION
Problems to be Solved by the Invention
[0022] As described above, regarding the whitening agent and the
anti-aging agent derived from plants, there is a desire to discover
a new plant which brings about new whitening and anti-aging
effects. The present invention has been completed in view of such
conventional circumstances, and an object of the present invention
is to provide a whitening agent, an anti-aging agent, and an
antioxidant agent derived from a new plant.
Means of Solving the Problems
[0023] The present inventors have performed extensive studies
taking the above circumstances into account, and as a result, have
found that there is a superior skin whitening activity and
antioxidation activity in a specified plant extract, whereby the
present invention has been completed.
[0024] The present invention is a whitening agent comprising an
extract of Psilotum spp., the family Psilotaceae.
[0025] The present invention is a skin external preparation for
whitening, comprising an extract of Psilotum spp., the family
Psilotaceae.
[0026] The present invention is an anti-aging agent comprising an
extract of Psilotum spp., the family Psilotaceae.
[0027] The present invention is a skin external preparation for
anti-aging, comprising an extract of Psilotum spp., the family
Psilotaceae.
[0028] The present invention is an antioxidant agent comprising an
extract of Psilotum spp., the family Psilotaceae.
[0029] The present invention is a skin external preparation for
antioxidation, comprising an extract of Psilotum spp., the family
Psilotaceae.
Effect of the Invention
[0030] The whitening agent of the present invention has a superior
skin whitening activity, has a superior effect in lightening and
whitening pigmentation, aging spots, freckles, chloasma, or the
like after sunburn.
[0031] The anti-aging agent and the antioxidant agent of the
present invention have a superior free radical scavenging ability
for preventing and controlling aging in the skin and skin diseases
caused by active oxygen (free radicals).
[0032] The skin external preparation for whitening of the present
invention can bring about a superior a whitening effect when
applied to the skin, has a superior effect in lightening and
whitening pigmentation, aging spots, freckles, chloasma, or the
like after sunburn, and has a high level of safety.
[0033] The skin external preparation for anti-aging and the skin
external preparation for antioxidation of the present invention can
bring about a superior free radical scavenging ability when applied
to the skin, can prevent and control aging in the skin and skin
diseases caused by active oxygen (free radicals), and has a high
level of safety.
BRIEF DESCRIPTION OF THE DRAWINGS
[0034] FIG. 1 is a drawing showing the melanogenesis-inhibitory
action of the plant extract of the present invention.
[0035] FIG. 2 is a drawing showing the radical elimination method
activity of the plant extract of the present invention.
MODE FOR CARRYING OUT THE INVENTION
[0036] The present invention will be explained below in detail.
[0037] The plant used in the present invention is a plant belonging
to the family Psilotaceae, the genus Psilotum, which is a cohort of
pteridophytes. As plants of the genus Psilotum, there are known
Psilotum nudum, Psilotum complanatum, Psilotum flabellatum,
Psilotum flaccidum, Psilotum triquetrum, Psilotum truncatum,
etc.
[0038] As a species which is distributed throughout Japan, Psilotum
nudum may be mentioned and is widely distributed in sub-central
Japan, Taiwan, southern China, Southeast Asia, etc. In the present
invention, specifically, Psilotum nudum is preferable as a plant
belonging to the family Psilotaceae, the genus Psilotum.
[0039] There had been no reports until the present inventors
initially discovered that there is a skin whitening activity, an
antioxidant activity, and an anti-aging activity in the plant
extract of the present invention.
[0040] The extract of the plant of the genus Psilotum used in the
present invention can be obtained by appropriately drying or
crushing the whole plant, followed by extraction with a solvent.
The extraction may be performed by standing still at room
temperature, or can be accelerated by heating, stirring, and
refluxing according to need. The obtained extract may be used
directly or used after a treatment such as filtering,
concentrating, or decoloring. Further, it is possible to use the
extract by removing the solvent followed by the re-dissolution in a
different solvent. It is also possible to use the extract by
further purifying the extract by charcoal, column chromatography,
etc.
[0041] As the extraction portion, other than the whole plant,
specific portions may be collected for extraction.
[0042] The extraction solvent used in the present invention may be
any solvent commonly used in extraction. Specifically, alcohol such
as methanol, ethanol, 1,3-butanediol, propylene glycol, and
dipropylene glycol; aqueous alcohol; an organic solvent such as
acetone, ethyl acetate, and chloroform can be used alone or in
combination. However, methanol, ethanol, 1,3-butanediol, acetone,
etc., are preferable. Further, these extracts may be purified by
solvent fractionation, charcoal treatment, column chromatography,
etc.
[0043] The whitening agent, the antioxidant agent, and the
anti-aging agent of the present invention are characterized by
comprising the extract of the plant of the genus Psilotum, but may
comprise other various components unless the effect of the present
invention is impaired.
[0044] The whitening agent or the anti-aging agent of the present
invention may be blended with a skin external preparation to from a
skin external preparation for whitening or a skin external
preparation for anti-aging on the basis of the antioxidant
activity. These skin external preparations can be optimally used in
the fields, specifically, cosmetics, pharmaceutical products, quasi
drugs, etc.
[0045] The amount of the plant extract in the skin external
preparation comprising the whitening agent or the anti-aging agent
of the present invention, as the dried residue of the components
derived from plants of the genus Psilotum, is normally 0.00001 mass
% or more, preferably, 0.0001 mass % or more. If the amount is too
small, the effect cannot be sufficiently brought about. The upper
limit is not specifically limited unless the effect of the present
invention is impaired. However, the excessive addition cannot bring
about a remarkable effect appropriate for the increase, and exerts
a bad influence in formulation design, usability, etc. Thus, the
amount is normally 10 mass % or less, more preferably, 5 mass % or
less.
[0046] The skin external preparation comprising the whitening agent
or the anti-aging agent of the present invention is prepared by
blending the whitening agent or the anti-aging agent with a base
for external use. Other than the aforementioned essential
components, the skin external preparation may appropriately
contain, if needed, components used in skin external preparations
as, e.g., general cosmetics and pharmaceutical products, for
example, moisturizer agents, antioxidants, oil ingredient,
ultraviolet rays absorbents, surfactants, thickening agents,
alcohols, powder ingredients, coloring materials, aqueous
ingredients, water, plant extracts, and various skin nutrients,
unless the effect of the present invention is impaired.
[0047] In addition, there may be appropriately blended sequestering
agents such as disodium edetate, trisodium edetate, sodium citrate,
sodium polyphosphate, sodium metaphosphate, and gluconic acid;
medicines such as caffeine, tannin, verapamil, tranexamic acid, and
their derivatives, licorice extract, glabridin, hot water extracts
of the fruit of firethorn, various herbal medicines, tocopherol
acetate, glycyrrhizinate, their derivatives, or their salts, other
whitening agents such as vitamin C, magnesium ascorbyl phosphate,
glucoside ascorbic acid, arbutin, kojic acid, alkoxybenzoic acid
and/or their salts; sugars such as glucose, fructose, mannose,
sucrose, trehalose, etc.
[0048] The skin external preparation comprising the whitening agent
or the anti-aging agent of the present invention may be in the form
of, for example, an ointment, cream, emulsion, lotion, pack, bath
agent, etc., conventional skin external preparations. The form is
not particularly limited.
[0049] The whitening agent or the anti-aging agent of the present
invention may be blended with food, as well as used in the
aforementioned preparations for external use. The intake of such
food is expected to bring about the whitening effect and the
anti-aging effect from inside.
EXAMPLES
[0050] The present invention will be further described below with
reference to the following examples, but the present invention is
not limited to the examples. The amounts are mass % unless
specifically indicated.
[0051] First, the method of preparation of the plant extract used
in the examples, and the testing methods and the results relating
to the melanogenesis inhibitory effect and the antioxidant effect
will be explained.
1. Preparation of Test Samples
[0052] All the plants used in the examples were from Okinawa, but
the plant of the present invention is not limited thereto.
(1) 75 ml of methanol was added to 7.4 g of the whole plant of
Psilotum nudum. Filtration was performed after immersion at room
temperature over seven days. The solvent of the filtrate was
removed by evaporation in vacuo, and 1.360 g of a dry solid
substance was obtained. (2) 10 ml of a 50% ethanol aqueous solution
was added to 1.04 g of the whole plant of Psilotum nudum.
Filtration was performed after extraction over three days while
stirring at room temperature. The solvent of the filtrate was
removed by evaporation in vacuo, and 0.18 g of a dry solid
substance was obtained. (3) 10 ml of a 70% ethanol aqueous solution
was added to 1.02 g of the whole plant of Psilotum nudum.
Filtration was performed after extraction over three days while
stirring at room temperature. The solvent of the filtrate was
removed by evaporation in vacuo, and 0.21 g of a dry solid
substance was obtained. (4) 10 ml of a 90% ethanol aqueous solution
was added to 1.03 g of the whole plant of Psilotum nudum.
Filtration was performed after extraction over three days while
stirring at room temperature. The solvent of the filtrate was
distilled, and 0.21 g of a dry solid substance was obtained. (5) 10
ml of 100% ethanol was added to 1.05 g of the whole plant of
Psilotum nudum. Filtration was performed after extraction over
three days while stirring at room temperature. The solvent of the
filtrate was removed by evaporation in vacuo, and 0.15 g of a dry
solid substance was obtained. (6) 10 ml of acetone was added to
1.00 g of the whole plant of Psilotum nudum. Filtration was
performed after extraction over three days while stirring at room
temperature. The solvent of the filtrate was removed by evaporation
in vacuo, and 0.072 g of a dry solid substance was obtained. (7) 10
ml of ethyl acetate was added to 1.01 g of the whole plant of
Psilotum nudum. Filtration was performed after extraction over
three days while stirring at room temperature. The solvent of the
filtrate was removed by evaporation in vacuo, and 0.067 g of a dry
solid substance was obtained. (8) 0 ml of 1,3-butanediol was added
to 1.00 g of the whole plant of Psilotum nudum. Filtration was
performed after extraction over three days while stirring at room
temperature. The evaporation residue (105.degree. C., under reduced
pressure for six hours) of the extract was measured to be 6.3 mg/g.
(9) 10 ml of dipropylene glycol was added to 1.00 g of the whole
plant of Psilotum nudum. Filtration was performed after extraction
over three days while stirring at room temperature. The evaporation
residue (105.degree. C., under reduced pressure for six hours) of
the extract was measured to be 5.3 mg/g. (10) 1.0 g of the dry
solid substance obtained in (1) was dispersed and dissolved in 50
ml of water, and then, extracted three times with 50 ml of ethyl
acetate. The ethyl acetate phase was concentrated and 272 mg of a
dried substance was obtained. The product was dissolved in acetone.
Filtration was performed after charcoal treatment. The filtrate was
concentrated and 201 mg of the discolored substance was obtained
(ethyl acetate fractionation).
2. Evaluation of Melanogenesis Inhibitory Effect (Method and
Results)
[0053] The Psilotum nudum extracts obtained by the respective
extraction methods were used as test samples, and the melanogenesis
inhibitory effect was measured and evaluated by the following
method.
(1) Cell Seeding and the Addition of Testing Substances
[0054] Mouse B16 melanoma cells were seeded in 6-well plates at
100,000 cells/well. The following day, the test sample (in the case
of a dried substance, solvent: DMSO) was added.
(2) Cell Proliferation Test
[0055] The culture medium was removed three days after the addition
of the test sample, and subsequently, 1 ml of EMEM culture medium
containing 10% alamar blue solution was added to the culture. After
incubation at 37.degree. C. for 30 minutes, 100 .mu.l of the medium
was transferred to a 96-well plate, and the fluorescence was
measured at a wavelength of 590 nm using an excitation wavelength
of 544 nm. Cell numbers were expressed as relative values to that
of the control (only the solvent containing no plant extract added)
by comparing the fluorescence intensities, which reflect the number
of viable cells.
[0056] The larger cell number indicates the lower toxicity of a
test sample. If the cell number of a test sample was less 80%, the
sample was considered as "cytotoxic".
(3) Determination of Melanin Amount
[0057] After the removal of the culture medium by aspiration, the
cells were washed with a buffer (phosphate buffer solution 50 mM,
pH 6.8), and subsequently, lysed by adding 1 M NaOH, and the
absorbance at 475 nm was measured. Melanin amounts were expressed
as relative values to that of the control (only the solvent
containing no plant extract added) by comparing the absorbances,
which reflect melanin amounts. The lower melanin amount indicates
the higher effect on melanogenesis inhibition.
[0058] Melanogenesis inhibitory effects for the respective plant
extracts are shown in Table 1. The concentrations of Psilotum nudum
extracts were shown as dried extract concentration unless otherwise
stated.
TABLE-US-00001 TABLE 1 Psilotum nudum 0 ppm 0.3 ppm 1 ppm 3 ppm 10
ppm extraction Melanin Cell Melanin Cell Melanin Cell Melanin Cell
Melanin Cell sample amount number amount number amount number
amount number amount number Methanol 100 100 87 96 80 97 49 104 18
87 extract 100% ethanol 100 100 109 98 99 102 95 99 53 110 extract
90% ethanol 100 100 100 98 98 99 85 106 47 112 extrac 50% ethanol
100 100 106 101 97 102 97 105 67 107 extract Acetone 100 100 95 100
90 99 68 103 30 93 extract
[0059] From FIG. 1, it is understood that the plant extracts used
in the present invention have a superior melanin inhibitory
activity and are useful as whitening agents. Numerous solvent
extracts other than those shown in Table 1 were evaluated, and a
high whitening effect was observed as shown in FIG. 1. FIG. 1 shows
the results when the respective solvent extracts were adjusted to a
final concentration of 0.04% (volume/volume). In each case,
cytotoxicity was not found. Note that, regarding the control, each
solvent alone was similarly adjusted to 0.04% (volume/volume).
[0060] Furthermore, it is understood from Table 2 that the ethyl
acetate fractionate of the methanol extract also showed a high
whitening effect.
TABLE-US-00002 TABLE 2 Psilotum nudum 0 ppm 0.3 ppm 1 ppm 3 ppm 10
ppm extraction Melanin Cell Melanin Cell Melanin Cell Melanin Cell
Melanin Cell sample amount number amount number amount number
amount number amount number Ethyl acetate 100 100 97 101 87 102 59
102 21 97 fractionation of the methanol extract
[0061] As described above, it is understood that the plant extracts
used in the present invention have a superior melanogenesis
inhibitory action, and are useful as whitening agents.
3. Evaluation and Results of the Antioxidant Effect
[0062] The Psilotum nudum extracts obtained by the respective
extraction methods were used as the test samples, and the
antioxidant effect was measured and evaluated by the following
method.
(1) Evaluation Method of Antioxidant Effect (DPPH radical Quenching
Assay)
[0063] The test substance dissolved in dimethyl sulfoxide was
injected into a 96-well plate at 10 .mu.l/well, and subsequently, 1
mmol/l of 1,1-diphenyl-2-picrylhydrazyl solution was added thereto
at 90 .mu.l/well. After leaving at room temperature for ten
minutes, the absorbency at 517 nm was determined, and the
scavenging ratio (%) of radicals to the amount in the control was
obtained from the data of the absorbency. The evaluation results
are shown in Table 3.
TABLE-US-00003 TABLE 3 DPPH radical scavenging ratio (%) Psilotum
nudum Concentration extract sample 0 ppm 10 ppm 30 ppm 100 ppm 300
ppm 1000 ppm 2000 ppm 50% ethanol extract 0 0 2 11 41 82 89 70%
ethanol extract 0 3 7 23 57 92 88 90% ethanol extract 0 6 17 43 81
78 75 100% ethanol extract 0 12 23 53 80 73 68 Acetone extract 0 9
32 78 78 72 61 Ethyl acetate extract 0 13 31 77 79 76 61
[0064] From Table 3, it is understood that, regarding the plant
extracts used in the present invention, the respective solvent
extracts have superior radical scavenging activities, and are
useful as antioxidant agents. Numerous solvent extracts other than
in Table 3 were evaluated, a high antioxidant effect as shown in
FIG. 2 was observed in the extract extracted with polyols such as
1,3-butanediol and dipropylene glycol. FIG. 2 shows the results
when each solvent extract was adjusted to a final concentration of
1% (volume/volume). In each case, cytotoxicity was not found.
[0065] From the above stated results, the extracts of the plant of
the genus Psilotum of the present invention show a superior
antioxidant effect, and thus, bring about a superior antioxidant
activity for human skin. Therefore, the plant extract, if blended
with an external use agent, can be used as an anti-aging agent for
preventing the aging of skin and maintaining the condition of
youthful and healthy skin.
[0066] Below, the application examples of the whitening agent and
the anti-aging agent in the various forms of the present invention
are explained as blending formulation examples. The present
invention is not limited to these formulation examples, and is,
needless to say, specified by the claims.
[0067] The Psilotum nudum extract amount in each formulation is
shown as the amount of dry residue after removal of the extraction
solvent.
TABLE-US-00004 mass % Blending formulation example 1 (Lotion)
trimethylglycine 1.0 Psilotum nudum ethanol extract 0.00001
glycerin 1.0 1,3-butylene glycol 5.0 sodium alginate 0.1 ethyl
alcohol 5.0 polyoxyethylene polyoxypropylene decyltetra decylether
0.2 sodium hexametaphosphate q.s. citric acid q.s. sodium citrate
q.s. phenoxyethanol q.s. fragrance q.s. purified water balance
Blending formulation example 2 (Lotion) Psilotum nudum acetone
extract, 1,3-butanediol 5.0 redissolved extract glycerin 2.0
1,3-butylene glycol 4.0 polyoxyethylene methyl glucoside 1.0
PEG/PPG-14/7 dimethyl ether 3.0 erythritol 1.0 polyoxyethylene
hydrogenated castor oil 0.5 polyglyceryl diisostearate 0.3
triethylhexanoin 0.3 trisodium EDTA q.s. citric acid q.s. sodium
citrate q.s. phenoxyethanol q.s. purified water balance Blending
formulation example 3 (lotion) tranexamic acid 1.0 potassium
4-methoxysalicylate 1.0 lipoic acid 0.1 Hamamelis leaf extract 0.1
hypotaurine 0.1 Sophora flavescens extract 0.1 Prunus perscia
extract 0.1 Beech bud extract 0.1 Psilotum nudum 1,3-butanediol
extract 0.0001 magnesium ascorbyl phosphate 0.1 thiotaurine 0.1
green tea extract 0.1 peppermint extract 0.1 Iris florentina root
extract 1.0 trimethylglycine 1.0 glycerin 1.0 1,3-butylene glycol
5.0 hydroxyethyl cellulose 0.05 ethyl alcohol 5.0 polyoxyethylene
polyoxypropylene decyltetra decylether 0.2 trisodium EDTA q.s.
citric acid q.s. sodium citrate q.s. phenoxyethanol q.s. fragrance
q.s. purified water balance Blending formulation example 5
(emulsion) dipotassium glycyrrhizinate 0.05 tocopherol acetate 0.5
Psilotum nudum, dipropylene glycol extract 0.001 sodium L-glutamate
0.05 fennel extract 0.1 yeast extract 0.1 Rehmannia chinensis root
extract 0.1 hydroxypropyl-.beta.-cyclodextrin 0.1 glycerin 6.0
1,3-butylene glycol 5.0 polyoxyethylene methyl glucoside 3.0
sunflower seed oil 1.0 squalene 2.0 isododecane 4.0
dimethylpolysiloxane 3.0 xanthane gum 0.1 carboxyvinyl polymer 0.1
acrylic acid-alkyl methacrylate copolymer 0.1 ethyl alcohol 5.0
potassium hydroxide q.s. sodium hexametaphosphate q.s. red iron
oxide q.s. yellow iron oxide q.s. ethyl paraben q.s. fragrance q.s.
purified water balance Blending formulation example 6 (Daily use
emulsion) sodium glycyrrhizinate 0.1 Psilotum nudum acetone
extract, ethanol redissolved 0.00003 extract tocopherol acetate 0.1
1,3-butylene glycol 5.0 squalene 0.5 isododecane 10.0 isohexadecane
25.0 dimethylpolysiloxane 2.0 polyoxyethylene - methylpolysiloxane
copolymer 1.5 trimethyl siloxysilicate 1.0
4-t-butyl-4'-methoxydibenzoylmethane 1.0 paramethoxy cinnaminic
acid 2-ethylhexyl 5.0 diparamethoxy cinnamic acid mono-2-glyceryl
1.0 ethylhexanoate silicone coated fine particle titanium oxide 4.0
dimethyl distearylammonium hectorite 0.5 spherical polyethylene
powder 3.0 talc 5.0 trisodium EDTA q.s. phenoxyethanol q.s.
fragrance q.s. purified water balance Blending formulation example
7 (emulsion) L-arginine 0.1 royal jelly extract 0.1 yeast extract
0.1 Psilotum nudum 90% ethanol extract 10.0 stearyl glycyrrhetinate
0.05 tocopherol acetate 0.1 sodium acetylated hyaluronate 0.1
glycerin 5.0 dipropylene glycol 7.0 polyethylene glycol 1500 2.0
liquid paraffin 7.0 vaseline 3.0 behenyl alcohol 1.0 batyl alcohol
2.0 jojoba oil 1.0 stearic acid 0.5 isostearic acid 0.5 behenic
acid 0.5 pentaerythritol tetra 2-ethylhexanoate 3.0 2-cetyl
ethylhexanoate 3.0 glycerin monostearate 1.0 polyoxyethylene
glycerin monostearate 1.0 carboxyvinyl polymer 0.15 sodium
hexametaphosphate q.s. potassium hydroxide q.s. methylparaben q.s.
fragrance q.s. purified water balance Blending formulation example
8 (emulsion) glucoside ascorbic acid 1.5 tranexamic acid 1.0
tocopherol acetate 0.1 sodium hyaluronate 0.05 Psilotum nudum
1,3-butanediol extract 0.0003 pantothenyl ethyl ether 0.1 stearyl
glycyrrhetinate 0.1 glycerin 7.0 1,3-butylene glycol 5.0
polyethylene glycol 20000 0.5 vaseline 2.0 jojoba oil 3.0 squalene
2.0 phytosteryl hydroxystearate 0.5 behenyl alcohol 0.5 batyl
alcohol 0.2 dimethylpolysiloxane 2.0 pentaerythritol tetra
2-ethylhexanoate 0.1 polyoxyethylene hydrogenated castor oil 1.0
polyoxyethylene glycerin isostearate 3.0
4-t-buytl-4'-methoxydibenzoylmethane 0.1 diparamethoxy cinnamic
acid mono 2-glyceryl 0.1 ethylhexanoate xanthane gum 0.1
carboxyvinyl polymer 0.2 ethanol 5.0 potassium hydroxide q.s.
sodium pyrosulfite q.s. sodium hexametaphosphate q.s. trisodium
EDTA q.s. yellow iron oxide q.s. paraoxy benzoate ester q.s.
purified water balance Blending formulation example 9 (cream)
Psilotum nudum 90% 1,3-butanediol extract 0.05 potassium
4-methoxysalicylate 3.0 propylene glycol 5.0 glycerin 8.0 stearic
acid 2.0 stearyl alcohol 7.0 hydrogenated lanolin 2.0 squalene 5.0
2-octyldodecyl alcohol 6.0 polyoxyethylene cetyl alcohol ether 3.0
glycerin monostearate ester 2.0 potassium hydroxide q.s. ethyl
paraben q.s. fragrance q.s. ion-exchange water balance Blending
formulation example 10 (cream) potassium 4-methoxysalicylate 1.0
3-O-ethyl ascorbic acid 1.0 Psilotum nudum 50% ethanol extract 0.3
Coenxyme Q10 0.03 tranexamic acid 2.0 tocopherol acetate 0.1 sodium
hyaluronate 0.05 pantothenyl ethyl ether 0.1 stearyl
glycyrrhetinate 0.1 glycerin 7.0 1,3-butylene glycol 5.0
polyethylene glycol 20000 0.5 vaseline 2.0 behenyl alcohol 0.5
batyl alcohol 0.2 squalene 2.0 phytosteryl hydroxystearate 0.5
jojoba oil 3.0 pentaerythritol tetra 2-ethylhexanoate 1.0
dimethylpolysiloxane 2.0 polyoxyethylene glycerin isostearate 1.5
polyoxyethylene hydrogenated castor oil 1.0 carboxyvinyl polymer
0.2 xanthane gum 0.1 ethanol 5.0 sodium hexametaphosphate q.s.
yellow iron oxide q.s. trisodium EDTA q.s. potassium hydroxide q.s.
paraoxy benzoate ester q.s. purified water balance Blending
formulation example 11(Two-layer type daytime use emulsion)
tranexamic acid 2.0 potassium 4-methoxysalicylate 1.0 Psilotum
nudum 50% 1,3-butanediol extract 3.0 dipotassium glycyrrhizinate
0.02 glutathione 1.0 thiotaurine 0.05 sophora flavescens extract
1.0 dipropylene glycol 5.0 dimethylpolysiloxane 5.0 isohexadecane
25.0 polyoxyethylene - methylpolysiloxane copolymer 2.0 dimethyl
distearylammonium hectorite 0.5 butyl ethyl propanediol 0.5
paramethoxy cinnaminic acid 2-ethylhexyl 7.5 trimethyl
siloxysilicate 5.0 spherical alkyl polyacrylate powder 5.0 dextrin
palmitate coated fine particle zinc oxide 15.0 trisodium EDTA q.s.
methylparaben q.s. phenoxyethanol q.s. fragrance q.s. purified
water balance Blending formulation example 12 (gel) potassium
4-methoxysalicylate 0.1 Lamium album extract 0.1
Psilotum nudum ethyl acetate fractionation dry matter 0.00001
dipotassium glycyrrhizinate 0.1 glucoside ascorbic acid 2.0
tocopherol acetate 0.1 Scutellaria baicalensis extract 0.1
saxifrage extract 0.1 glycerin 2.0 1,3-butylene glycol 5.0
polyethylene glycol 1500 3.0 polyethylene glycol 20000 3.0 agar
powder 1.5 xanthane gum 0.3 acrylic acid-alkyl methacrylate
copolymer 0.05 cetyl octanoate 3.0 dimethylpolysiloxane 5.0 sodium
hexametaphosphate q.s. dibutyl hydroxytoluene q.s. yellow iron
oxide q.s. citric acid q.s. sodium citrate q.s. sodium hydroxide
q.s. phenoxyethanol q.s. fragrance q.s. purified water balance
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