U.S. patent application number 13/080279 was filed with the patent office on 2011-07-28 for delta-8 desaturase and its use in making polyunsaturated fatty acids. This patent application is currently assigned to E.I. du Pont de Nemours and Company. Invention is credited to Howard Glenn DAMUDE, Quinn Qun Zhu.
Application Number | 20110184200 13/080279 |
Document ID | / |
Family ID | 38626732 |
Filed Date | 2011-07-28 |
United States Patent Application | 20110184200 |
Kind Code | A1 |
DAMUDE; Howard Glenn ; et al. | July 28, 2011 |
Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.
Inventors: | DAMUDE; Howard Glenn; (Hockessin, DE) ; Zhu; Quinn Qun; (West Chester, PA) |
Assignee: | E.I. du Pont de Nemours and
Company Wilmington DE |
Family ID: | 38626732 |
Appl. No.: | 13/080279 |
Filed: | April 5, 2011 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
11737772 | Apr 20, 2007 | 7943823 | ||
13080279 | ||||
60795810 | Apr 28, 2006 | |||
60837789 | Aug 15, 2006 | |||
Current U.S. Class: | 554/84 |
Current CPC Class: | C12N 15/8247 20130101; C12N 9/0083 20130101 |
Class at Publication: | 554/84 |
International Class: | C07F 9/02 20060101 C07F009/02 |
Sequence CWU 1
1
78119DNAArtificial SequenceT7 primer 1ggaaacagct atgaccatg
192459PRTRhizopus stolonifer 2Met Ser Thr Leu Asp Arg Gln Ser Ile
Phe Thr Ile Lys Glu Leu Glu1 5 10 15Ser Ile Ser Gln Arg Ile His Asp
Gly Asp Glu Glu Ala Met Lys Phe 20 25 30Ile Ile Ile Asp Lys Lys Val
Tyr Asp Val Thr Glu Phe Ile Glu Asp 35 40 45His Pro Gly Gly Ala Gln
Val Leu Leu Thr His Val Gly Lys Asp Ala 50 55 60Ser Asp Val Phe His
Ala Met His Pro Glu Ser Ala Tyr Glu Val Leu65 70 75 80Asn Asn Tyr
Phe Val Gly Asp Val Gln Glu Thr Val Val Thr Glu Lys 85 90 95Ser Ser
Ser Ala Gln Phe Ala Val Glu Met Arg Gln Leu Arg Asp Gln 100 105
110Leu Lys Lys Glu Gly Tyr Phe His Ser Ser Lys Leu Phe Tyr Ala Tyr
115 120 125Lys Val Leu Ser Thr Leu Ala Ile Cys Ile Ala Gly Leu Ser
Leu Leu 130 135 140Tyr Ala Tyr Gly Arg Thr Ser Thr Leu Ala Val Val
Ala Ser Ala Ile145 150 155 160Thr Val Gly Ile Phe Trp Gln Gln Cys
Gly Trp Leu Ala His Asp Phe 165 170 175Gly His His Gln Cys Phe Glu
Asp Arg Thr Trp Asn Asp Val Leu Val 180 185 190Val Phe Leu Gly Asn
Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp Lys 195 200 205Asn Lys His
Asn Thr His His Ala Ser Thr Asn Val His Gly Gln Asp 210 215 220Pro
Asp Ile Asp Thr Ala Pro Val Leu Leu Trp Asp Glu Tyr Ala Ser225 230
235 240Ala Ala Tyr Tyr Ala Ser Leu Asp Gln Glu Pro Thr Met Val Ser
Arg 245 250 255Phe Leu Ala Glu Gln Val Leu Pro His Gln Thr Arg Tyr
Phe Phe Phe 260 265 270Ile Leu Ala Phe Ala Arg Leu Ser Trp Ala Leu
Gln Ser Leu Ser Tyr 275 280 285Ser Phe Lys Lys Glu Ser Ile Asn Lys
Ser Arg Gln Leu Asn Leu Phe 290 295 300Glu Arg Val Cys Ile Val Gly
His Trp Ala Leu Phe Ala Phe Cys Ile305 310 315 320Tyr Ser Trp Cys
Ser Asn Val Tyr His Met Val Leu Phe Phe Leu Val 325 330 335Ser Gln
Ala Thr Thr Gly Tyr Thr Leu Ala Leu Val Phe Ala Leu Asn 340 345
350His Asn Gly Met Pro Val Ile Thr Glu Glu Lys Ala Glu Ser Met Glu
355 360 365Phe Phe Glu Ile Gln Val Ile Thr Gly Arg Asp Val Thr Leu
Ser Pro 370 375 380Leu Gly Asp Trp Phe Met Gly Gly Leu Asn Tyr Gln
Ile Glu His His385 390 395 400Val Phe Pro Asn Met Pro Arg His Asn
Leu Pro Thr Val Lys Pro Met 405 410 415Val Lys Ser Leu Cys Gln Lys
Tyr Asp Ile Asn Tyr His Asp Thr Gly 420 425 430Phe Leu Lys Gly Thr
Leu Glu Val Leu Gln Thr Leu Asp Ile Thr Ser 435 440 445Lys Leu Ser
Leu Gln Leu Ser Lys Lys Ser Phe 450 4553695DNAPavlova lutheri
3agggccaagg gtgccaacca ccttccacgt gagactacac accgtaggcc gatgggcaag
60ggtggagacg gcggcgcgca ggcggtgagc gggaccgacg cgtctctcgc tgaggtgagc
120tccgtcgata gcaagagcgt gcacgtcgtg ctctacggca agcgcgtgga
tgtcacaaag 180ttccagggct acgacgtggc ctggtggcgc gcgcgccata
acacgcacca cgtgtgcacc 240aacgaggatg gttcggaccc ggacatcaag
acggcgcccc tgctcatcta cgtgcgagag 300aacccgtcca ttgccaagcg
gctcaacttc ttccagcgct ggcagcagta ctactatgtg 360ccgaccatgg
ccatcctcga cctctactgg cgcctggagt ccatcgcgta cgtggctgtg
420cgcctgccta agatgtggat gcaggccgcc gctcttgccg ctcactacgc
gctcctgtgc 480tgggtcttcg cagcgcatct caacctcatc cctctcatga
tggttgcacg cggcttcgcg 540acgggcatcg ttgtctttgc aacccactat
ggtgaggaca tcctcgaccg cgagcacgtc 600gagggcatga cgctcgtcga
gcagaccgcc aagacctccc gtaacatcac gggcggctgg 660ctagtgaacg
tgctcacggg cttcatctcc ctgca 69541106DNAPavlova lutheri 4agggccaagg
gtgccaacca ccttccacgt gagactacac accgtaggcc gatgggcaag 60ggtggagacg
gcggcgcgca ggcggtgagc gggaccgacg cgtctctcgc tgaggtgagc
120tccgtcgata gcaagagcgt gcacgtcgtg ctctacggca agcgcgtgga
tgtcacaaag 180ttccagggct acgacgtggc ctggtggcgc gcgcgccata
acacgcacca cgtgtgcacc 240aacgaggatg gttcggaccc ggacatcaag
acggcgcccc tgctcatcta cgtgcgagag 300aacccgtcca ttgccaagcg
gctcaacttc ttccagcgct ggcagcagta ctactatgtg 360ccgaccatgg
ccatcctcga cctctactgg cgcctggagt ccatcgcgta cgtggctgtg
420cgcctgccta agatgtggat gcaggccgcc gctcttgccg ctcactacgc
gctcctgtgc 480tgggtcttcg cagcgcatct caacctcatc cctctcatga
tggttgcacg cggcttcgcg 540acgggcatcg ttgtctttgc aacccactat
ggtgaggaca tcctcgaccg cgagcacgtc 600gagggcatga cgctcgtcga
gcagaccgcc aagacctccc gtaacatcac gggcggctgg 660ctagtgaacg
tgctcacggg cttcatctcc ctgcagaccg agcatcacct cttccccatg
720atgcccaccg gcaacctaat gactatccag cccgaggtac gcgacttctt
caagaagcat 780ggcctcgagt accgcgaggg caacctcttc cagtgcgtgc
accagaacat caaggctctc 840gccttcgagc acctcctcca ctgagcgtca
ccactcaagc gtcctaagtg cacaggtact 900gtcttctgac cgatggccgc
gcggctccct cggctggcag tggggccaac gagtggcctc 960gcgggatcgg
gcacgatcgg gcctccatga aacttcagtg ttcagagaca agccgacaac
1020ctccgcatcg tgagaaatct tttaaagcag tatgttccat cacgccgctt
ttgcagtcaa 1080taacattacc caaaaaaaaa aaaaaa 11065287PRTPavlova
lutheri 5Arg Ala Lys Gly Ala Asn His Leu Pro Arg Glu Thr Thr His
Arg Arg1 5 10 15Pro Met Gly Lys Gly Gly Asp Gly Gly Ala Gln Ala Val
Ser Gly Thr 20 25 30Asp Ala Ser Leu Ala Glu Val Ser Ser Val Asp Ser
Lys Ser Val His 35 40 45Val Val Leu Tyr Gly Lys Arg Val Asp Val Thr
Lys Phe Gln Gly Tyr 50 55 60Asp Val Ala Trp Trp Arg Ala Arg His Asn
Thr His His Val Cys Thr65 70 75 80Asn Glu Asp Gly Ser Asp Pro Asp
Ile Lys Thr Ala Pro Leu Leu Ile 85 90 95Tyr Val Arg Glu Asn Pro Ser
Ile Ala Lys Arg Leu Asn Phe Phe Gln 100 105 110Arg Trp Gln Gln Tyr
Tyr Tyr Val Pro Thr Met Ala Ile Leu Asp Leu 115 120 125Tyr Trp Arg
Leu Glu Ser Ile Ala Tyr Val Ala Val Arg Leu Pro Lys 130 135 140Met
Trp Met Gln Ala Ala Ala Leu Ala Ala His Tyr Ala Leu Leu Cys145 150
155 160Trp Val Phe Ala Ala His Leu Asn Leu Ile Pro Leu Met Met Val
Ala 165 170 175Arg Gly Phe Ala Thr Gly Ile Val Val Phe Ala Thr His
Tyr Gly Glu 180 185 190Asp Ile Leu Asp Arg Glu His Val Glu Gly Met
Thr Leu Val Glu Gln 195 200 205Thr Ala Lys Thr Ser Arg Asn Ile Thr
Gly Gly Trp Leu Val Asn Val 210 215 220Leu Thr Gly Phe Ile Ser Leu
Gln Thr Glu His His Leu Phe Pro Met225 230 235 240Met Pro Thr Gly
Asn Leu Met Thr Ile Gln Pro Glu Val Arg Asp Phe 245 250 255Phe Lys
Lys His Gly Leu Glu Tyr Arg Glu Gly Asn Leu Phe Gln Cys 260 265
270Val His Gln Asn Ile Lys Ala Leu Ala Phe Glu His Leu Leu His 275
280 285620DNAArtificial Sequenceprimer SeqE 6cgacacactc caatctttcc
20720DNAArtificial Sequenceprimer SeqW 7ggtggctgga gttagacatc
208457PRTMortierella alpina 8Met Ala Ala Ala Pro Ser Val Arg Thr
Phe Thr Arg Ala Glu Ile Leu1 5 10 15Asn Ala Glu Ala Leu Asn Glu Gly
Lys Lys Asp Ala Glu Ala Pro Phe 20 25 30Leu Met Ile Ile Asp Asn Lys
Val Tyr Asp Val Arg Glu Phe Val Pro 35 40 45Asp His Pro Gly Gly Ser
Val Ile Leu Thr His Val Gly Lys Asp Gly 50 55 60Thr Asp Val Phe Asp
Thr Phe His Pro Glu Ala Ala Trp Glu Thr Leu65 70 75 80Ala Asn Phe
Tyr Val Gly Asp Ile Asp Glu Ser Asp Arg Ala Ile Lys 85 90 95Asn Asp
Asp Phe Ala Ala Glu Val Arg Lys Leu Arg Thr Leu Phe Gln 100 105
110Ser Leu Gly Tyr Tyr Asp Ser Ser Lys Ala Tyr Tyr Ala Phe Lys Val
115 120 125Ser Phe Asn Leu Cys Ile Trp Gly Leu Ser Thr Phe Ile Val
Ala Lys 130 135 140Trp Gly Gln Thr Ser Thr Leu Ala Asn Val Leu Ser
Ala Ala Leu Leu145 150 155 160Gly Leu Phe Trp Gln Gln Cys Gly Trp
Leu Ala His Asp Phe Leu His 165 170 175His Gln Val Phe Gln Asp Arg
Phe Trp Gly Asp Leu Phe Gly Ala Phe 180 185 190Leu Gly Gly Val Cys
Gln Gly Phe Ser Ser Ser Trp Trp Lys Asp Lys 195 200 205His Asn Thr
His His Ala Ala Pro Asn Val His Gly Glu Asp Pro Asp 210 215 220Ile
Asp Thr His Pro Leu Leu Thr Trp Ser Glu His Ala Leu Glu Met225 230
235 240Phe Ser Asp Val Pro Asp Glu Glu Leu Thr Arg Met Trp Ser Arg
Phe 245 250 255Met Val Leu Asn Gln Thr Trp Phe Tyr Phe Pro Ile Leu
Ser Phe Ala 260 265 270Arg Leu Ser Trp Cys Leu Gln Ser Ile Met Phe
Val Leu Pro Asn Gly 275 280 285Gln Ala His Lys Pro Ser Gly Ala Arg
Val Pro Ile Ser Leu Val Glu 290 295 300Gln Leu Ser Leu Ala Met His
Trp Thr Trp Tyr Leu Ala Thr Met Phe305 310 315 320Leu Phe Ile Lys
Asp Pro Val Asn Met Ile Val Tyr Phe Leu Val Ser 325 330 335Gln Ala
Val Cys Gly Asn Leu Leu Ala Ile Val Phe Ser Leu Asn His 340 345
350Asn Gly Met Pro Val Ile Ser Lys Glu Glu Ala Val Asp Met Asp Phe
355 360 365Phe Thr Lys Gln Ile Ile Thr Gly Arg Asp Val His Pro Gly
Leu Phe 370 375 380Ala Asn Trp Phe Thr Gly Gly Leu Asn Tyr Gln Ile
Glu His His Leu385 390 395 400Phe Pro Ser Met Pro Arg His Asn Phe
Ser Lys Ile Gln Pro Ala Val 405 410 415Glu Thr Leu Cys Lys Lys Tyr
Gly Val Arg Tyr His Thr Thr Gly Met 420 425 430Ile Glu Gly Thr Ala
Glu Val Phe Ser Arg Leu Asn Glu Val Ser Lys 435 440 445Ala Ala Ser
Lys Met Gly Lys Ala Gln 450 455922DNAArtificial Sequenceprimer AP1
9gtaatacgac tcactatagg gc 221020DNAArtificial Sequenceprimer GSP
PvDES 10ctgcgaagac ccagcacagg 201122DNAArtificial Sequenceprimer
M13-28Rev 11gtaatacgac tcactatagg gc 221221DNAArtificial
Sequenceprimer PavDes seq 12ttgtggcgct caatcatctc c
21131294DNAPavlova lutheri 13ctttgcgagc gcggcgcaga cgattgcggc
ccgtagtgat cgcggtgcgc attgctgtgt 60ttctagtttt gctgacgccc ggcccgataa
tgacaccttc tcccgtttga aatactaata 120agtaactata ttataatatt
caaaggtggc gactatggat ctccttttct aaagttcagc 180ggaattggga
atcggagaaa tttcgagata tgtcataatc acgtgctcta tctcgaatga
240accgcggccg gtgagcgatt actcgggaag ccaattccta ttaacgagtc
aggggggatc 300tttgaggtga gtcggccacg cagagagagc aaggaatcat
cctcatccgc cgttctcgag 360aaagagccaa gggtgccaac caccttccac
gtgagactac acaccgtagg ccgatgggca 420agggtggaga cggcggcgcg
caggcggcga gcgggaccga cgcatctctc gctgaggtga 480gctccgtcga
tagcaagagc gtgcgcgtcg tgctctacgg caagcgcgtg gatgtcacaa
540agttccagag ggcacacccg ggcgggagca aggtgttccg catcttccag
gagcgcgacg 600cgacggagca gttcgagtct taccactcgc ccaaggccat
caagatgatg gagggcatgc 660tcaagaagtc ggaggatgcg cccgcttccg
tgcccctgcc ctcgcggtcc accatgggca 720cggagttcaa ggagatgatt
gagcgccaca agagggctgg tctctacgac ccttgcccgt 780tggacgagct
gttcaagctc accatcgtcc ttgcgcccat cttcgtgggc gcctatctcg
840tgcggagcgg cgtctcgccc ctcgcgggcg cgctctccat gggctttggc
ttctacctcg 900acggctggct tgctcacrac tacctgcatc acgcagtctt
caagggctcg gtcaacacgc 960tcgtcaaggc gaacaacgcc atgggatacg
ccctcggctt cctccagggc tacgacgtgg 1020cctggtggcg cgcgcgccat
aacacgcacc acgtgtgcac caacgaggat ggttcggacc 1080cggacatcaa
gacggcgccc ctgctcatct acgtgcgaga gaacccgtcc attgccaagc
1140ggctcaactt cttccagcgc tggcagcagt actactatgt gccgaccatg
gccatcctcg 1200acctctactg gcgcctggag tccatcgcgt acgtggctgt
gcgcctgcct aagatgtgga 1260tgcaggccgc cgctcttgcc gctcactacg cgct
1294141927DNAPavlova lutheri 14ctttgcgagc gcggcgcaga cgattgcggc
ccgtagtgat cgcggtgcgc attgctgtgt 60ttctagtttt gctgacgccc ggcccgataa
tgacaccttc tcccgtttga aatactaata 120agtaactata ttataatatt
caaaggtggc gactatggat ctccttttct aaagttcagc 180ggaattggga
atcggagaaa tttcgagata tgtcataatc acgtgctcta tctcgaatga
240accgcggccg gtgagcgatt actcgggaag ccaattccta ttaacgagtc
aggggggatc 300tttgaggtga gtcggccacg cagagagagc aaggaatcat
cctcatccgc cgttctcgag 360aaagagccaa gggtgccaac caccttccac
gtgagactac acaccgtagg ccgatgggca 420agggtggaga cggcggcgcg
caggcggtga gcgggaccga cgcgtctctc gctgaggtga 480gctccgtcga
tagcaagagc gtgcacgtcg tgctctacgg caagcgcgtg gatgtcacaa
540agttccagaa ggcacacccg ggcgggagca aggtgttccg catcttccag
gagcgcgacg 600cgacggagca gttcgagtct taccactcgc ccaaggccat
caagatgatg gagggcatgc 660tcaagaagtc ggaggatgcg cccgcttccg
tgcccctgcc ctcgcggtcc accatgggca 720cggagttcaa ggagatgatt
gagcgccaca agagggctgg tctctacgac ccttgcccgt 780tggacgagct
gttcaagctc accatcgtcc ttgcgcccat cttcgtgggc gcctatctcg
840tgcggagcgg cgtctcgccc ctcgcgggcg cgctctccat gggctttggc
ttctacctcg 900acggctggct tgctcacgac tacctgcatc acgcagtctt
caagggctcg gtcaacacgc 960tcgtcaaggc gaacaacgcc atgggatacg
ccctcggctt cctccagggc tacgacgtgg 1020cctggtggcg cgcgcgccat
aacacgcacc acgtgtgcac caacgaggat ggttcggacc 1080cggacatcaa
gacggcgccc ctgctcatct acgtgcgaga gaacccgtcc attgccaagc
1140ggctcaactt cttccagcgc tggcagcagt actactatgt gccgaccatg
gccatcctcg 1200acctctactg gcgcctggag tccatcgcgt acgtggctgt
gcgcctgcct aagatgtgga 1260tgcaggccgc cgctcttgcc gctcactacg
cgctcctgtg ctgggtcttc gcagcgcatc 1320tcaacctcat ccctctcatg
atggttgcac gcggcttcgc gacgggcatc gttgtctttg 1380caacccacta
tggtgaggac atcctcgacc gcgagcacgt cgagggcatg acgctcgtcg
1440agcagaccgc caagacctcc cgtaacatca cgggcggctg gctagtgaac
gtgctcacgg 1500gcttcatctc cctgcagacc gagcatcacc tcttccccat
gatgcccacc ggcaacctaa 1560tgactatcca gcccgaggta cgcgacttct
tcaagaagca tggcctcgag taccgcgagg 1620gcaacctctt ccagtgcgtg
caccagaaca tcaaggctct cgccttcgag cacctcctcc 1680actgagcgtc
accactcaag cgtcctaagt gcacaggtac tgtcttctga ccgatggccg
1740cgcggctccc tcggctggca gtggggccaa cgagtggcct cgcgggatcg
ggcacgatcg 1800ggcctccatg aaacttcagt gttcagagac aagccgacaa
cctccgcatc gtgagaaatc 1860ttttaaagca gtatgttcca tcacgccgct
tttgcagtca ataacattac ccaaaaaaaa 1920aaaaaaa 1927151269DNAPavlova
lutheri 15atgggcaagg gtggagacgg cggcgcgcag gcggtgagcg ggaccgacgc
gtctctcgct 60gaggtgagct ccgtcgatag caagagcgtg cacgtcgtgc tctacggcaa
gcgcgtggat 120gtcacaaagt tccagaaggc acacccgggc gggagcaagg
tgttccgcat cttccaggag 180cgcgacgcga cggagcagtt cgagtcttac
cactcgccca aggccatcaa gatgatggag 240ggcatgctca agaagtcgga
ggatgcgccc gcttccgtgc ccctgccctc gcggtccacc 300atgggcacgg
agttcaagga gatgattgag cgccacaaga gggctggtct ctacgaccct
360tgcccgttgg acgagctgtt caagctcacc atcgtccttg cgcccatctt
cgtgggcgcc 420tatctcgtgc ggagcggcgt ctcgcccctc gcgggcgcgc
tctccatggg ctttggcttc 480tacctcgacg gctggcttgc tcacgactac
ctgcatcacg cagtcttcaa gggctcggtc 540aacacgctcg tcaaggcgaa
caacgccatg ggatacgccc tcggcttcct ccagggctac 600gacgtggcct
ggtggcgcgc gcgccataac acgcaccacg tgtgcaccaa cgaggatggt
660tcggacccgg acatcaagac ggcgcccctg ctcatctacg tgcgagagaa
cccgtccatt 720gccaagcggc tcaacttctt ccagcgctgg cagcagtact
actatgtgcc gaccatggcc 780atcctcgacc tctactggcg cctggagtcc
atcgcgtacg tggctgtgcg cctgcctaag 840atgtggatgc aggccgccgc
tcttgccgct cactacgcgc tcctgtgctg ggtcttcgca 900gcgcatctca
acctcatccc tctcatgatg gttgcacgcg gcttcgcgac gggcatcgtt
960gtctttgcaa cccactatgg tgaggacatc ctcgaccgcg agcacgtcga
gggcatgacg 1020ctcgtcgagc agaccgccaa gacctcccgt aacatcacgg
gcggctggct agtgaacgtg 1080ctcacgggct tcatctccct gcagaccgag
catcacctct tccccatgat gcccaccggc 1140aacctaatga ctatccagcc
cgaggtacgc gacttcttca agaagcatgg cctcgagtac 1200cgcgagggca
acctcttcca gtgcgtgcac cagaacatca aggctctcgc cttcgagcac
1260ctcctccac 126916423PRTPavlova lutheri 16Met Gly Lys Gly Gly Asp
Gly Gly Ala Gln Ala Val Ser Gly Thr Asp1 5 10 15Ala Ser Leu Ala Glu
Val Ser Ser Val Asp Ser Lys Ser Val His Val 20 25 30Val Leu Tyr Gly
Lys Arg Val Asp Val Thr Lys Phe Gln Lys Ala His 35 40 45Pro Gly Gly
Ser Lys Val Phe Arg Ile Phe Gln Glu Arg Asp Ala Thr 50 55 60Glu Gln
Phe Glu Ser Tyr
His Ser Pro Lys Ala Ile Lys Met Met Glu65 70 75 80Gly Met Leu Lys
Lys Ser Glu Asp Ala Pro Ala Ser Val Pro Leu Pro 85 90 95Ser Arg Ser
Thr Met Gly Thr Glu Phe Lys Glu Met Ile Glu Arg His 100 105 110Lys
Arg Ala Gly Leu Tyr Asp Pro Cys Pro Leu Asp Glu Leu Phe Lys 115 120
125Leu Thr Ile Val Leu Ala Pro Ile Phe Val Gly Ala Tyr Leu Val Arg
130 135 140Ser Gly Val Ser Pro Leu Ala Gly Ala Leu Ser Met Gly Phe
Gly Phe145 150 155 160Tyr Leu Asp Gly Trp Leu Ala His Asp Tyr Leu
His His Ala Val Phe 165 170 175Lys Gly Ser Val Asn Thr Leu Val Lys
Ala Asn Asn Ala Met Gly Tyr 180 185 190Ala Leu Gly Phe Leu Gln Gly
Tyr Asp Val Ala Trp Trp Arg Ala Arg 195 200 205His Asn Thr His His
Val Cys Thr Asn Glu Asp Gly Ser Asp Pro Asp 210 215 220Ile Lys Thr
Ala Pro Leu Leu Ile Tyr Val Arg Glu Asn Pro Ser Ile225 230 235
240Ala Lys Arg Leu Asn Phe Phe Gln Arg Trp Gln Gln Tyr Tyr Tyr Val
245 250 255Pro Thr Met Ala Ile Leu Asp Leu Tyr Trp Arg Leu Glu Ser
Ile Ala 260 265 270Tyr Val Ala Val Arg Leu Pro Lys Met Trp Met Gln
Ala Ala Ala Leu 275 280 285Ala Ala His Tyr Ala Leu Leu Cys Trp Val
Phe Ala Ala His Leu Asn 290 295 300Leu Ile Pro Leu Met Met Val Ala
Arg Gly Phe Ala Thr Gly Ile Val305 310 315 320Val Phe Ala Thr His
Tyr Gly Glu Asp Ile Leu Asp Arg Glu His Val 325 330 335Glu Gly Met
Thr Leu Val Glu Gln Thr Ala Lys Thr Ser Arg Asn Ile 340 345 350Thr
Gly Gly Trp Leu Val Asn Val Leu Thr Gly Phe Ile Ser Leu Gln 355 360
365Thr Glu His His Leu Phe Pro Met Met Pro Thr Gly Asn Leu Met Thr
370 375 380Ile Gln Pro Glu Val Arg Asp Phe Phe Lys Lys His Gly Leu
Glu Tyr385 390 395 400Arg Glu Gly Asn Leu Phe Gln Cys Val His Gln
Asn Ile Lys Ala Leu 405 410 415Ala Phe Glu His Leu Leu His
42017459PRTRhizopus stolonifer 17Met Ser Thr Leu Asp Arg Gln Ser
Ile Phe Thr Ile Lys Glu Leu Glu1 5 10 15Ser Ile Ser Gln Arg Ile His
Asp Gly Asp Glu Glu Ala Met Lys Phe 20 25 30Ile Ile Ile Asp Lys Lys
Val Tyr Asp Val Thr Glu Phe Ile Glu Asp 35 40 45His Pro Gly Gly Ala
Gln Val Leu Leu Thr His Val Gly Lys Asp Ala 50 55 60Ser Asp Val Phe
His Ala Met His Pro Glu Ser Ala Tyr Glu Val Leu65 70 75 80Asn Asn
Tyr Phe Val Gly Asp Val Gln Glu Thr Val Val Thr Glu Lys 85 90 95Ser
Ser Ser Ala Gln Phe Ala Val Glu Met Arg Gln Leu Arg Asp Gln 100 105
110Leu Lys Lys Glu Gly Tyr Phe His Ser Ser Lys Leu Phe Tyr Ala Tyr
115 120 125Lys Val Leu Ser Thr Leu Ala Ile Cys Ile Ala Gly Leu Ser
Pro Leu 130 135 140Tyr Ala Tyr Gly Arg Thr Ser Thr Leu Ala Val Val
Ala Ser Ala Ile145 150 155 160Thr Val Gly Ile Phe Trp Gln Gln Cys
Gly Trp Leu Ala His Asp Phe 165 170 175Gly His His Gln Cys Phe Glu
Asp Arg Thr Trp Asn Asp Val Leu Val 180 185 190Val Phe Leu Gly Asn
Phe Cys Gln Gly Phe Ser Leu Ser Trp Trp Lys 195 200 205Asn Lys His
Asn Thr His His Ala Ser Thr Asn Val His Gly Gln Asp 210 215 220Pro
Asp Ile Asp Thr Ala Pro Val Leu Leu Trp Asp Glu Tyr Ala Ser225 230
235 240Ala Ala Tyr Tyr Ala Ser Leu Asp Gln Glu Pro Thr Met Val Ser
Arg 245 250 255Phe Leu Ala Glu Gln Val Leu Pro His Gln Thr Arg Tyr
Phe Phe Phe 260 265 270Ile Leu Ala Phe Ala Arg Leu Ser Trp Ala Leu
Gln Ser Leu Ser Tyr 275 280 285Ser Phe Lys Lys Glu Ser Ile Asn Lys
Ser Arg Gln Leu Asn Leu Phe 290 295 300Glu Arg Val Cys Ile Val Gly
His Trp Ala Leu Ser Ala Phe Cys Ile305 310 315 320Tyr Ser Trp Cys
Ser Asn Val Tyr His Met Val Leu Phe Phe Leu Val 325 330 335Ser Gln
Ala Thr Thr Gly Tyr Thr Leu Ala Leu Val Phe Ala Leu Asn 340 345
350His Asn Gly Met Pro Val Ile Thr Glu Glu Lys Ala Glu Ser Met Glu
355 360 365Phe Phe Glu Ile Gln Val Ile Thr Gly Arg Asp Val Thr Leu
Ser Pro 370 375 380Leu Gly Asp Trp Phe Met Gly Gly Leu Asn Tyr Gln
Ile Glu His His385 390 395 400Val Phe Pro Asn Met Pro Arg His Asn
Leu Pro Thr Val Lys Pro Met 405 410 415Val Lys Ser Leu Cys Gln Lys
Tyr Asp Ile Asn Tyr His Asp Thr Gly 420 425 430Phe Leu Lys Gly Thr
Leu Glu Val Leu Gln Thr Leu Asp Ile Thr Ser 435 440 445Lys Leu Ser
Leu Gln Leu Ser Lys Lys Ser Phe 450 4551830DNAArtificial
Sequenceprimer PvDES5'Not-1 18gcggccgcac catgggcaag ggtggagacg
301927DNAArtificial Sequenceprimer PvDES3'Not-1 19gcggccgctc
agtggaggag gtgctcg 272020DNAArtificial Sequenceprimer GSP PvDES-2
20gcatccacat cttaggcagg 20218801DNAArtificial Sequenceplasmid pY121
21ggccgcaggg cggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg
60acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
120ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
acttaatcgc 180cttgcagcac atcccccttt cgccagctgg cgtaatagcg
aagaggcccg caccgatcgc 240ccttcccaac agttgcgcag cctgaatggc
gaatggcgcg acgcgccctg tagcggcgca 300ttaagcgcgg cgggtgtggt
ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 360gcgcccgctc
ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
420caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
gcacctcgac 480cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
catcgccctg atagacggtt 540tttcgccctt tgacgttgga gtccacgttc
tttaatagtg gactcttgtt ccaaactgga 600acaacactca accctatctc
ggtctattct tttgatttat aagggatttt gccgatttcg 660gcctattggt
taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
720ttaacgttta caatttcctg atgcggtatt ttctccttac gcatctgtgc
ggtatttcac 780accgcatatc gacggtcgag gagaacttct agtatatcca
catacctaat attattgcct 840tattaaaaat ggaatcccaa caattacatc
aaaatccaca ttctcttcaa aatcaattgt 900cctgtacttc cttgttcatg
tgtgttcaaa aacgttatat ttataggata attatactct 960atttctcaac
aagtaattgg ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat
1020atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag
agttcgaatc 1080tcttagcaac cattattttt ttcctcaaca taacgagaac
acacaggggc gctatcgcac 1140agaatcaaat tcgatgactg gaaatttttt
gttaatttca gaggtcgcct gacgcatata 1200cctttttcaa ctgaaaaatt
gggagaaaaa ggaaaggtga gaggccggaa ccggcttttc 1260atatagaata
gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa
1320tattatttaa ggacctattg ttttttccaa taggtggtta gcaatcgtct
tactttctaa 1380cttttcttac cttttacatt tcagcaatat atatatatat
ttcaaggata taccattcta 1440atgtctgccc ctatgtctgc ccctaagaag
atcgtcgttt tgccaggtga ccacgttggt 1500caagaaatca cagccgaagc
cattaaggtt cttaaagcta tttctgatgt tcgttccaat 1560gtcaagttcg
atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc
1620ccacttccag atgaggcgct ggaagcctcc aagaaggttg atgccgtttt
gttaggtgct 1680gtggctggtc ctaaatgggg taccggtagt gttagacctg
aacaaggttt actaaaaatc 1740cgtaaagaac ttcaattgta cgccaactta
agaccatgta actttgcatc cgactctctt 1800ttagacttat ctccaatcaa
gccacaattt gctaaaggta ctgacttcgt tgttgtcaga 1860gaattagtgg
gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct
1920tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
ggccgctttc 1980atggccctac aacatgagcc accattgcct atttggtcct
tggataaagc taatcttttg 2040gcctcttcaa gattatggag aaaaactgtg
gaggaaacca tcaagaacga attccctaca 2100ttgaaggttc aacatcaatt
gattgattct gccgccatga tcctagttaa gaacccaacc 2160cacctaaatg
gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
2220tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc
tttgccagac 2280aagaacaccg catttggttt gtacgaacca tgccacggtt
ctgctccaga tttgccaaag 2340aataaggttg accctatcgc cactatcttg
tctgctgcaa tgatgttgaa attgtcattg 2400aacttgcctg aagaaggtaa
ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt 2460atcagaactg
gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
2520gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata
tttgtacata 2580aactttataa atgaaattca taatagaaac gacacgaaat
tacaaaatgg aatatgttca 2640tagggtagac gaaactatat acgcaatcta
catacattta tcaagaagga gaaaaaggag 2700gatagtaaag gaatacaggt
aagcaaattg atactaatgg ctcaacgtga taaggaaaaa 2760gaattgcact
ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt
2820aacagaaaat catgaaacta cgattcctaa tttgatattg gaggattttc
tctaaaaaaa 2880aaaaaataca acaaataaaa aacactcaat gacctgacca
tttgatggag tttaagtcaa 2940taccttcttg aagcatttcc cataatggtg
aaagttccct caagaatttt actctgtcag 3000aaacggcctt acgacgtagt
cgatatggtg cactctcagt acaatctgct ctgatgccgc 3060atagttaagc
cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
3120gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
tgtgtcagag 3180gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
ctcgtgatac gcctattttt 3240ataggttaat gtcatgataa taatggtttc
ttagtatgat ccaatatcaa aggaaatgat 3300agcattgaag gatgagacta
atccaattga ggagtggcag catatagaac agctaaaggg 3360tagtgctgaa
ggaagcatac gataccccgc atggaatggg ataatatcac aggaggtact
3420agactacctt tcatcctaca taaatagacg catataagta cgcatttaag
cataaacacg 3480cactatgccg ttcttctcat gtatatatat atacaggcaa
cacgcagata taggtgcgac 3540gtgaacagtg agctgtatgt gcgcagctcg
cgttgcattt tcggaagcgc tcgttttcgg 3600aaacgctttg aagttcctat
tccgaagttc ctattctcta gaaagtatag gaacttcaga 3660gcgcttttga
aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg
3720gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg
ctcaaaagta 3780tctctttgct atatatctct gtgctatatc cctatataac
ctacccatcc acctttcgct 3840ccttgaactt gcatctaaac tcgacctcta
cattttttat gtttatctct agtattactc 3900tttagacaaa aaaattgtag
taagaactat tcatagagtg aatcgaaaac aatacgaaaa 3960tgtaaacatt
tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt
4020ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg
cgcaatccac 4080atcggtatag aatataatcg gggatgcctt tatcttgaaa
aaatgcaccc gcagcttcgc 4140tagtaatcag taaacgcggg aagtggagtc
aggctttttt tatggaagag aaaatagaca 4200ccaaagtagc cttcttctaa
ccttaacgga cctacagtgc aaaaagttat caagagactg 4260cattatagag
cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc
4320gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt
tggtaaaata 4380gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct
cagattcttt gtttgaaaaa 4440ttagcgctct cgcgttgcat ttttgtttta
caaaaatgaa gcacagattc ttcgttggta 4500aaatagcgct ttcgcgttgc
atttctgttc tgtaaaaatg cagctcagat tctttgtttg 4560aaaaattagc
gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
4620caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
ttctaaatac 4680attcaaatat gtatccgctc atgagacaat aaccctgata
aatgcttcaa taatattgaa 4740aaaggaagag tatgagtatt caacatttcc
gtgtcgccct tattcccttt tttgcggcat 4800tttgccttcc tgtttttgct
cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 4860agttgggtgc
acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
4920gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
ctatgtggcg 4980cggtattatc ccgtattgac gccgggcaag agcaactcgg
tcgccgcata cactattctc 5040agaatgactt ggttgagtac tcaccagtca
cagaaaagca tcttacggat ggcatgacag 5100taagagaatt atgcagtgct
gccataacca tgagtgataa cactgcggcc aacttacttc 5160tgacaacgat
cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5220taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
gacgagcgtg 5280acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
actattaact ggcgaactac 5340ttactctagc ttcccggcaa caattaatag
actggatgga ggcggataaa gttgcaggac 5400cacttctgcg ctcggccctt
ccggctggct ggtttattgc tgataaatct ggagccggtg 5460agcgtgggtc
tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
5520tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
cagatcgctg 5580agataggtgc ctcactgatt aagcattggt aactgtcaga
ccaagtttac tcatatatac 5640tttagattga tttaaaactt catttttaat
ttaaaaggat ctaggtgaag atcctttttg 5700ataatctcat gaccaaaatc
ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 5760tagaaaagat
caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
5820aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
ctaccaactc 5880tttttccgaa ggtaactggc ttcagcagag cgcagatacc
aaatactgtc cttctagtgt 5940agccgtagtt aggccaccac ttcaagaact
ctgtagcacc gcctacatac ctcgctctgc 6000taatcctgtt accagtggct
gctgccagtg gcgataagtc gtgtcttacc gggttggact 6060caagacgata
gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6120agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
gagctatgag 6180aaagcgccac gcttcccgaa gggagaaagg cggacaggta
tccggtaagc ggcagggtcg 6240gaacaggaga gcgcacgagg gagcttccag
ggggaaacgc ctggtatctt tatagtcctg 6300tcgggtttcg ccacctctga
cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 6360gcctatggaa
aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6420ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
attaccgcct 6480ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
gcgcagcgag tcagtgagcg 6540aggaagcgga agagcgccca atacgcaaac
cgcctctccc cgcgcgttgg ccgattcatt 6600aatgcagctg gcacgacagg
tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 6660atgtgagtta
cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta
6720tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
gaccatgatt 6780acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
gctggagctc caccgcggga 6840tttcgaaact aagttcttgg tgttttaaaa
ctaaaaaaaa gactaactat aaaagtagaa 6900tttaagaagt ttaagaaata
gatttacaga attacaatca atacctaccg tctttatata 6960cttattagtc
aagtagggga ataatttcag ggaactggtt tcaacctttt ttttcagctt
7020tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga
tagatacatg 7080cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag
gttgcatcac tccattgagg 7140ttgtgcccgt tttttgcctg tttgtgcccc
tgttctctgt agttgcgcta agagaatgga 7200cctatgaact gatggttggt
gaagaaaaca atattttggt gctgggattc tttttttttc 7260tggatgccag
cttaaaaagc gggctccatt atatttagtg gatgccagga ataaactgtt
7320cacccagaca cctacgatgt tatatattct gtgtaacccg ccccctattt
tgggcatgta 7380cgggttacag cagaattaaa aggctaattt tttgactaaa
taaagttagg aaaatcacta 7440ctattaatta tttacgtatt ctttgaaatg
gcagtattga taatgataaa ctcgaaatca 7500ctagtggatc cgcccagcgg
ccgcaccatg ggcaagggtg gagacggcgg cgcgcaggcg 7560gtgagcggga
ccgacgcgtc tctcgctgag gtgagctccg tcgatagcaa gagcgtgcac
7620gtcgtgctct acggcaagcg cgtggatgtc acaaagttcc agaaggcaca
cccgggcggg 7680agcaaggtgt tccgcatctt ccaggagcgc gacgcgacgg
agcagttcga gtcttaccac 7740tcgcccaagg ccatcaagat gatggagggc
atgctcaaga agtcggagga tgcgcccgct 7800tccgtgcccc tgccctcgcg
gtccaccatg ggcacggagt tcaaggagat gattgagcgc 7860cacaagaggg
ctggtctcta cgacccttgc ccgttggacg agctgttcaa gctcaccatc
7920gtccttgcgc ccatcttcgt gggcgcctat ctcgtgcgga gcggcgtctc
gcccctcgcg 7980ggcgcgctct ccatgggctt tggcttctac ctcgacggct
ggcttgctca cgactacctg 8040catcacgcag tcttcaaggg ctcggtcaac
acgctcgtca aggcgaacaa cgccatggga 8100tacgccctcg gcttcctcca
gggctacgac gtggcctggt ggcgcgcgcg ccataacacg 8160caccacgtgt
gcaccaacga ggatggttcg gacccggaca tcaagacggc gcccctgctc
8220atctacgtgc gagagaaccc gtccattgcc aagcggctca acttcttcca
gcgctggcag 8280cagtactact atgtgccgac catggccatc ctcgacctct
actggcgcct ggagtccatc 8340gcgtacgtgg ctgtgcgcct gcctaagatg
tggatgcagg ccgccgctct tgccgctcac 8400tacgcgctcc tgtgctgggt
cttcgcagcg catctcaacc tcatccctct catgatggtt 8460gcacgcggct
tcgcgacggg catcgttgtc tttgcaaccc actatggtga ggacatcctc
8520gaccgcgagc acgtcgaggg catgacgctc gtcgagcaga ccgccaagac
ctcccgtaac 8580atcacgggcg gctggctagt gaacgtgctc acgggcttca
tctccctgca gaccgagcat 8640cacctcttcc ccatgatgcc caccggcaac
ctaatgacta tccagcccga ggtacgcgac 8700ttcttcaaga agcatggcct
cgagtaccgc gagggcaacc tcttccagtg cgtgcaccag 8760aacatcaagg
ctctcgcctt cgagcacctc ctccactgag c 8801224993DNAArtificial
Sequenceplasmid pKR123r 22ctagacctgc aggatataat gagccgtaaa
caaagatgat taagtagtaa ttaatacgta 60ctagtaaaag tggcaaaaga taacgagaaa
gaaccaattt ctttgcattc ggccttagcg 120gaaggcatat ataagctttg
attattttat ttagtgtaat gatttcgtac aaccaaagca 180tttatttagt
actctcacac ttgtgtcgcg gccgcttggg gggctatgga agactttctt
240agttagttgt gtgaataagc aatgttggga gaatcgggac tacttatagg
ataggaataa 300aacagaaaag tattaagtgc taatgaaata tttagactga
taattaaaat cttcacgtat 360gtccacttga tataaaaacg tcaggaataa
aggaagtaca gtagaattta aaggtactct 420ttttatatat acccgtgttc
tctttttggc tagctagttg cataaaaaat aatctatatt 480tttatcatta
ttttaaatat cttatgagat ggtaaatatt tatcataatt ttttttacta
540ttatttatta tttgtgtgtg taatacatat agaagttaat tacaaatttt
atttactttt 600tcattatttt gatatgattc accattaatt tagtgttatt
atttataata gttcatttta 660atctttttgt atatattatg cgtgcagtac
ttttttccta catataacta ctattacatt 720ttatttatat aatattttta
ttaatgaatt ttcgtgataa tatgtaatat tgttcattat 780tatttcagat
tttttaaaaa tatttgtgtt attatttatg aaatatgtaa tttttttagt
840atttgatttt atgatgataa agtgttctaa attcaaaaga
agggggaaag cgtaaacatt 900aaaaaacgtc atcaaacaaa aacaaaatct
tgttaataaa gataaaactg tttgttttga 960tcactgttat ttcgtaatat
aaaaacatta tttatattta tattgttgac aaccaaattt 1020gcctatcaaa
tctaaccaat ataatgcatg cgtggcaggt aatgtactac catgaactta
1080agtcatgaca taataaaccg tgaatctgac caatgcatgt acctanctaa
attgtatttg 1140tgacacgaag caaatgattc aattcacaat ggagatggga
aacaaataat gaagaaccca 1200gaactaagaa agcttttctg aaaaataaaa
taaaggcaat gtcaaaagta tactgcatca 1260tcagtccaga aagcacatga
tattttttta tcagtatcaa tgcagctagt tttattttac 1320aatatcgata
tagctagttt aaatatattg cagctagatt tataaatatt tgtgttatta
1380tttatcattt gtgtaatcct gtttttagta ttttagttta tatatgatga
taatgtattc 1440caaatttaaa agaagggaaa taaatttaaa caagaaaaaa
agtcatcaaa caaaaaacaa 1500atgaaagggt ggaaagatgt taccatgtaa
tgtgaatgtt acagtatttc ttttattata 1560gagttaacaa attaactaat
atgattttgt taataatgat aaaatatttt ttttattatt 1620atttcataat
ataaaaatag tttacttaat ataaaaaaaa ttctatcgtt cacaacaaag
1680ttggccacct aatttaacca tgcatgtacc catggaccat attaggtaac
catcaaacct 1740gatgaagaga taaagagatg aagacttaag tcataacaca
aaaccataaa aaacaaaaat 1800acaatcaacc gtcaatctga ccaatgcatg
aaaaagctgc aatagtgagt ggcgacacaa 1860agcacatgat tttcttacaa
cggagataaa accaaaaaaa tatttcatga acaacctaga 1920acaaataaag
cttttatata ataaatatat aaataaataa aggctatgga ataatatact
1980tcaatatatt tggattaaat aaattgttgg cggggttgat atatttatac
acacctaaag 2040tcacttcaat ctcattttca cttaactttt attttttttt
tctttttatt tatcataaag 2100agaatattga taatatactt tttaacatat
ttttatgaca ttttttattg gtgaaaactt 2160attaaaaatc ataaattttg
taagttagat ttatttaaag agttcctctt cttattttaa 2220attttttaat
aaatttttaa ataactaaaa tttgtgttaa aaatgttaaa aaatgtgtta
2280ttaacccttc tcttcgagga cgtacgtcta gagtcgacct gcaggcatgc
aagcttggcg 2340taatcatggt catagctgtt tcctgtgtga aattgttatc
cgctcacaat tccacacaac 2400atacgagccg gaagcataaa gtgtaaagcc
tggggtgcct aatgagtgag ctaactcaca 2460ttaattgcgt tgcgctcact
gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 2520taatgaatcg
gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
2580tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
agctcactca 2640aaggcggtaa tacggttatc cacagaatca ggggataacg
caggaaagaa catgtgagca 2700aaaggccagc aaaaggccag gaaccgtaaa
aaggccgcgt tgctggcgtt tttccatagg 2760ctccgccccc ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 2820acaggactat
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2880ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
cgtggcgctt 2940tctcatagct cacgctgtag gtatctcagt tcggtgtagg
tcgttcgctc caagctgggc 3000tgtgtgcacg aaccccccgt tcagcccgac
cgctgcgcct tatccggtaa ctatcgtctt 3060gagtccaacc cggtaagaca
cgacttatcg ccactggcag cagccactgg taacaggatt 3120agcagagcga
ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
3180tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
cttcggaaaa 3240agagttggta gctcttgatc cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt 3300tgcaagcagc agattacgcg cagaaaaaaa
ggatctcaag aagatccttt gatcttttct 3360acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta 3420tcaaaaagga
tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3480agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc 3540tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact 3600acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc 3660tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt 3720ggtcctgcaa
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
3780agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
catcgtggtg 3840tcacgctcgt cgtttggtat ggcttcattc agctccggtt
cccaacgatc aaggcgagtt 3900acatgatccc ccatgttgtg caaaaaagcg
gttagctcct tcggtcctcc gatcgttgtc 3960agaagtaagt tggccgcagt
gttatcactc atggttatgg cagcactgca taattctctt 4020actgtcatgc
catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
4080tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
ggataatacc 4140gcgccacata gcagaacttt aaaagtgctc atcattggaa
aacgttcttc ggggcgaaaa 4200ctctcaagga tcttaccgct gttgagatcc
agttcgatgt aacccactcg tgcacccaac 4260tgatcttcag catcttttac
tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 4320aatgccgcaa
aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
4380tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
catatttgaa 4440tgtatttaga aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct 4500gacgtctaag aaaccattat tatcatgaca
ttaacctata aaaataggcg tatcacgagg 4560ccctttcgtc tcgcgcgttt
cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 4620gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
4680tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
gcagattgta 4740ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
gcgtaaggag aaaataccgc 4800atcaggcgcc attcgccatt caggctgcgc
aactgttggg aagggcgatc ggtgcgggcc 4860tcttcgctat tacgccagct
ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 4920acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
4980acccggggat cct 4993236276DNAArtificial Sequenceplasmid pKR900
23ggccgcgaca caagtgtgag agtactaaat aaatgctttg gttgtacgaa atcattacac
60taaataaaat aatcaaagct tatatatgcc ttccgctaag gccgaatgca aagaaattgg
120ttctttctcg ttatcttttg ccacttttac tagtacgtat taattactac
ttaatcatct 180ttgtttacgg ctcattatat cctgcaggtc tagaggatcc
ccgggtaccg agctcgaatt 240cactggccgt cgttttacaa cgtcgtgact
gggaaaaccc tggcgttacc caacttaatc 300gccttgcagc acatccccct
ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 360gcccttccca
acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
420ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
atctgctctg 480atgccgcata gttaagccag ccccgacacc cgccaacacc
cgctgacgcg ccctgacggg 540cttgtctgct cccggcatcc gcttacagac
aagctgtgac cgtctccggg agctgcatgt 600gtcagaggtt ttcaccgtca
tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 660tatttttata
ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
720ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
aatatgtatc 780cgctcatgag acaataaccc tgataaatgc ttcaataata
ttgaaaaagg aagagtatga 840gtattcaaca tttccgtgtc gcccttattc
ccttttttgc ggcattttgc cttcctgttt 900ttgctcaccc agaaacgctg
gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 960tgggttacat
cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
1020aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
ttatcccgta 1080ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
ttctcagaat gacttggttg 1140agtactcacc agtcacagaa aagcatctta
cggatggcat gacagtaaga gaattatgca 1200gtgctgccat aaccatgagt
gataacactg cggccaactt acttctgaca acgatcggag 1260gaccgaagga
gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
1320gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
acgatgcctg 1380tagcaatggc aacaacgttg cgcaaactat taactggcga
actacttact ctagcttccc 1440ggcaacaatt aatagactgg atggaggcgg
ataaagttgc aggaccactt ctgcgctcgg 1500cccttccggc tggctggttt
attgctgata aatctggagc cggtgagcgt gggtctcgcg 1560gtatcattgc
agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
1620cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
ggtgcctcac 1680tgattaagca ttggtaactg tcagaccaag tttactcata
tatactttag attgatttaa 1740aacttcattt ttaatttaaa aggatctagg
tgaagatcct ttttgataat ctcatgacca 1800aaatccctta acgtgagttt
tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 1860gatcttcttg
agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1920cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
ccgaaggtaa 1980ctggcttcag cagagcgcag ataccaaata ctgtccttct
agtgtagccg tagttaggcc 2040accacttcaa gaactctgta gcaccgccta
catacctcgc tctgctaatc ctgttaccag 2100tggctgctgc cagtggcgat
aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 2160cggataaggc
gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
2220gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
gccacgcttc 2280ccgaagggag aaaggcggac aggtatccgg taagcggcag
ggtcggaaca ggagagcgca 2340cgagggagct tccaggggga aacgcctggt
atctttatag tcctgtcggg tttcgccacc 2400tctgacttga gcgtcgattt
ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 2460ccagcaacgc
ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
2520ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
tgagctgata 2580ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
gagcgaggaa gcggaagagc 2640gcccaatacg caaaccgcct ctccccgcgc
gttggccgat tcattaatgc agctggcacg 2700acaggtttcc cgactggaaa
gcgggcagtg agcgcaacgc aattaatgtg agttagctca 2760ctcattaggc
accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
2820tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
aagcttgcat 2880gcctgcaggt cgactctaga cgtacgtcct cgaagagaag
ggttaataac acatttttta 2940acatttttaa cacaaatttt agttatttaa
aaatttatta aaaaatttaa aataagaaga 3000ggaactcttt aaataaatct
aacttacaaa atttatgatt tttaataagt tttcaccaat 3060aaaaaatgtc
ataaaaatat gttaaaaagt atattatcaa tattctcttt atgataaata
3120aaaagaaaaa aaaaataaaa gttaagtgaa aatgagattg aagtgacttt
aggtgtgtat 3180aaatatatca accccgccaa caatttattt aatccaaata
tattgaagta tattattcca 3240tagcctttat ttatttatat atttattata
taaaagcttt atttgttcta ggttgttcat 3300gaaatatttt tttggtttta
tctccgttgt aagaaaatca tgtgctttgt gtcgccactc 3360actattgcag
ctttttcatg cattggtcag attgacggtt gattgtattt ttgtttttta
3420tggttttgtg ttatgactta agtcttcatc tctttatctc ttcatcaggt
ttgatggtta 3480cctaatatgg tccatgggta catgcatggt taaattaggt
ggccaacttt gttgtgaacg 3540atagaatttt ttttatatta agtaaactat
ttttatatta tgaaataata ataaaaaaaa 3600tattttatca ttattaacaa
aatcatatta gttaatttgt taactctata ataaaagaaa 3660tactgtaaca
ttcacattac atggtaacat ctttccaccc tttcatttgt tttttgtttg
3720atgacttttt ttcttgttta aatttatttc ccttctttta aatttggaat
acattatcat 3780catatataaa ctaaaatact aaaaacagga ttacacaaat
gataaataat aacacaaata 3840tttataaatc tagctgcaat atatttaaac
tagctatatc gatattgtaa aataaaacta 3900gctgcattga tactgataaa
aaaatatcat gtgctttctg gactgatgat gcagtatact 3960tttgacattg
cctttatttt atttttcaga aaagctttct tagttctggg ttcttcatta
4020tttgtttccc atctccattg tgaattgaat catttgcttc gtgtcacaaa
tacaatttag 4080ntaggtacat gcattggtca gattcacggt ttattatgtc
atgacttaag ttcatggtag 4140tacattacct gccacgcatg cattatattg
gttagatttg ataggcaaat ttggttgtca 4200acaatataaa tataaataat
gtttttatat tacgaaataa cagtgatcaa aacaaacagt 4260tttatcttta
ttaacaagat tttgtttttg tttgatgacg ttttttaatg tttacgcttt
4320cccccttctt ttgaatttag aacactttat catcataaaa tcaaatacta
aaaaaattac 4380atatttcata aataataaca caaatatttt taaaaaatct
gaaataataa tgaacaatat 4440tacatattat cacgaaaatt cattaataaa
aatattatat aaataaaatg taatagtagt 4500tatatgtagg aaaaaagtac
tgcacgcata atatatacaa aaagattaaa atgaactatt 4560ataaataata
acactaaatt aatggtgaat catatcaaaa taatgaaaaa gtaaataaaa
4620tttgtaatta acttctatat gtattacaca cacaaataat aaataatagt
aaaaaaaatt 4680atgataaata tttaccatct cataagatat ttaaaataat
gataaaaata tagattattt 4740tttatgcaac tagctagcca aaaagagaac
acgggtatat ataaaaagag tacctttaaa 4800ttctactgta cttcctttat
tcctgacgtt tttatatcaa gtggacatac gtgaagattt 4860taattatcag
tctaaatatt tcattagcac ttaatacttt tctgttttat tcctatccta
4920taagtagtcc cgattctccc aacattgctt attcacacaa ctaactaaga
aagtcttcca 4980tagcccccca agcggccgca ccatgggcaa gggtggagac
ggcggcgcgc aggcggtgag 5040cgggaccgac gcgtctctcg ctgaggtgag
ctccgtcgat agcaagagcg tgcacgtcgt 5100gctctacggc aagcgcgtgg
atgtcacaaa gttccagaag gcacacccgg gcgggagcaa 5160ggtgttccgc
atcttccagg agcgcgacgc gacggagcag ttcgagtctt accactcgcc
5220caaggccatc aagatgatgg agggcatgct caagaagtcg gaggatgcgc
ccgcttccgt 5280gcccctgccc tcgcggtcca ccatgggcac ggagttcaag
gagatgattg agcgccacaa 5340gagggctggt ctctacgacc cttgcccgtt
ggacgagctg ttcaagctca ccatcgtcct 5400tgcgcccatc ttcgtgggcg
cctatctcgt gcggagcggc gtctcgcccc tcgcgggcgc 5460gctctccatg
ggctttggct tctacctcga cggctggctt gctcacgact acctgcatca
5520cgcagtcttc aagggctcgg tcaacacgct cgtcaaggcg aacaacgcca
tgggatacgc 5580cctcggcttc ctccagggct acgacgtggc ctggtggcgc
gcgcgccata acacgcacca 5640cgtgtgcacc aacgaggatg gttcggaccc
ggacatcaag acggcgcccc tgctcatcta 5700cgtgcgagag aacccgtcca
ttgccaagcg gctcaacttc ttccagcgct ggcagcagta 5760ctactatgtg
ccgaccatgg ccatcctcga cctctactgg cgcctggagt ccatcgcgta
5820cgtggctgtg cgcctgccta agatgtggat gcaggccgcc gctcttgccg
ctcactacgc 5880gctcctgtgc tgggtcttcg cagcgcatct caacctcatc
cctctcatga tggttgcacg 5940cggcttcgcg acgggcatcg ttgtctttgc
aacccactat ggtgaggaca tcctcgaccg 6000cgagcacgtc gagggcatga
cgctcgtcga gcagaccgcc aagacctccc gtaacatcac 6060gggcggctgg
ctagtgaacg tgctcacggg cttcatctcc ctgcagaccg agcatcacct
6120cttccccatg atgcccaccg gcaacctaat gactatccag cccgaggtac
gcgacttctt 6180caagaagcat ggcctcgagt accgcgaggg caacctcttc
cagtgcgtgc accagaacat 6240caaggctctc gccttcgagc acctcctcca ctgagc
6276245303DNAArtificial Sequenceprimer pKR325 24agcttggatc
tcctgcagga tctggccggc cggatctcgt acggatccgt cgacggcgcg 60cccgatcatc
cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
120gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc
agcttccttt 180cgggctttgt tagcagccgg atcgatccaa gctgtacctc
actattcctt tgccctcgga 240cgagtgctgg ggcgtcggtt tccactatcg
gcgagtactt ctacacagcc atcggtccag 300acggccgcgc ttctgcgggc
gatttgtgta cgcccgacag tcccggctcc ggatcggacg 360attgcgtcgc
atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
420tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
cgatcctgca 480agctccggat gcctccgctc gaagtagcgc gtctgctgct
ccatacaagc caaccacggc 540ctccagaaga agatgttggc gacctcgtat
tgggaatccc cgaacatcgc ctcgctccag 600tcaatgaccg ctgttatgcg
gccattgtcc gtcaggacat tgttggagcc gaaatccgcg 660tgcacgaggt
gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
720tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
cacatgggga 780tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
gaccgattcc ttgcggtccg 840aatgggccga acccgctcgt ctggctaaga
tcggccgcag cgatcgcatc catagcctcc 900gcgaccggct gcagaacagc
gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc 960tgtgcacggc
gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact
1020tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
tttgtagaaa 1080ccatcggcgc agctatttac ccgcaggaca tatccacgcc
ctcctacatc gaagctgaaa 1140gcacgagatt cttcgccctc cgagagctgc
atcaggtcgg agacgctgtc gaacttttcg 1200atcagaaact tctcgacaga
cgtcgcggtg agttcaggct tttccatggg tatatctcct 1260tcttaaagtt
aaacaaaatt atttctagag ggaaaccgtt gtggtctccc tatagtgagt
1320cgtattaatt tcgcgggatc gagatcgatc caattccaat cccacaaaaa
tctgagctta 1380acagcacagt tgctcctctc agagcagaat cgggtattca
acaccctcat atcaactact 1440acgttgtgta taacggtcca catgccggta
tatacgatga ctggggttgt acaaaggcgg 1500caacaaacgg cgttcccgga
gttgcacaca agaaatttgc cactattaca gaggcaagag 1560cagcagctga
cgcgtacaca acaagtcagc aaacagacag gttgaacttc atccccaaag
1620gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
ccaaagcaaa 1680aagcccactg gctcacgcta ggaaccaaaa ggcccagcag
tgatccagcc ccaaaagaga 1740tctcctttgc cccggagatt acaatggacg
atttcctcta tctttacgat ctaggaagga 1800agttcgaagg tgaaggtgac
gacactatgt tcaccactga taatgagaag gttagcctct 1860tcaatttcag
aaagaatgct gacccacaga tggttagaga ggcctacgca gcaggtctca
1920tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
aagaaggtta 1980aagatgcagt caaaagattc aggactaatt gcatcaagaa
cacagagaaa gacatatttc 2040tcaagatcag aagtactatt ccagtatgga
cgattcaagg cttgcttcat aaaccaaggc 2100aagtaataga gattggagtc
tctaaaaagg tagttcctac tgaatctaag gccatgcatg 2160gagtctaaga
ttcaaatcga ggatctaaca gaactcgccg tgaagactgg cgaacagttc
2220atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
ggtggagcac 2280gacactctgg tctactccaa aaatgtcaaa gatacagtct
cagaagacca aagggctatt 2340gagacttttc aacaaaggat aatttcggga
aacctcctcg gattccattg cccagctatc 2400tgtcacttca tcgaaaggac
agtagaaaag gaaggtggct cctacaaatg ccatcattgc 2460gataaaggaa
aggctatcat tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
2520ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
aaagcaagtg 2580gattgatgtg acatctccac tgacgtaagg gatgacgcac
aatcccacta tccttcgcaa 2640gacccttcct ctatataagg aagttcattt
catttggaga ggacacgctc gagctcattt 2700ctctattact tcagccataa
caaaagaact cttttctctt cttattaaac catgaaaaag 2760cctgaactca
ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
2820gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
tgtaggaggg 2880cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
tctacaaaga tcgttatgtt 2940tatcggcact ttgcatcggc cgcgctcccg
attccggaag tgcttgacat tggggaattc 3000agcgagagcc tgacctattg
catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg 3060cctgaaaccg
aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
3120gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
aatcggtcaa 3180tacactacat ggcgtgattt catatgcgcg attgctgatc
cccatgtgta tcactggcaa 3240actgtgatgg acgacaccgt cagtgcgtcc
gtcgcgcagg ctctcgatga gctgatgctt 3300tgggccgagg actgccccga
agtccggcac ctcgtgcacg cggatttcgg ctccaacaat 3360gtcctgacgg
acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
3420gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
ttgtatggag 3480cagcagacgc gctacttcga gcggaggcat ccggagcttg
caggatcgcc gcggctccgg 3540gcgtatatgc tccgcattgg tcttgaccaa
ctctatcaga gcttggttga cggcaatttc 3600gatgatgcag cttgggcgca
gggtcgatgc gacgcaatcg tccgatccgg agccgggact 3660gtcgggcgta
cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
3720gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
ggaatagtga 3780ggtacctaaa gaaggagtgc gtcgaagcag atcgttcaaa
catttggcaa taaagtttct 3840taagattgaa tcctgttgcc ggtcttgcga
tgattatcat ataatttctg ttgaattacg 3900ttaagcatgt aataattaac
atgtaatgca tgacgttatt tatgagatgg gtttttatga 3960ttagagtccc
gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
4020aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgatgtcga
atcgatcaac 4080ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
tgcgtattgg gcgctcttcc 4140gcttcctcgc tcactgactc gctgcgctcg
gtcgttcggc tgcggcgagc ggtatcagct 4200cactcaaagg cggtaatacg
gttatccaca gaatcagggg ataacgcagg aaagaacatg 4260tgagcaaaag
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
4320cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
gaggtggcga 4380aacccgacag gactataaag ataccaggcg tttccccctg
gaagctccct cgtgcgctct 4440cctgttccga ccctgccgct taccggatac
ctgtccgcct ttctcccttc gggaagcgtg 4500gcgctttctc aatgctcacg
ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 4560ctgggctgtg
tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
4620cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
cactggtaac 4680aggattagca gagcgaggta tgtaggcggt gctacagagt
tcttgaagtg gtggcctaac 4740tacggctaca ctagaaggac agtatttggt
atctgcgctc tgctgaagcc agttaccttc 4800ggaaaaagag ttggtagctc
ttgatccggc aaacaaacca ccgctggtag cggtggtttt 4860tttgtttgca
agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
4920ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
tttggtcatg 4980acattaacct ataaaaatag gcgtatcacg aggccctttc
gtctcgcgcg tttcggtgat 5040gacggtgaaa acctctgaca catgcagctc
ccggagacgg tcacagcttg tctgtaagcg 5100gatgccggga gcagacaagc
ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 5160tggcttaact
atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg
5220tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
acgatttagg 5280tgacactata gaacggcgcg cca 5303258898DNAArtificial
Sequenceplasmid pKR902 25ggatctggcc ggccggatct cgtacggatc
cgtcgacggc gcgcccgatc atccggatat 60agttcctcct ttcagcaaaa aacccctcaa
gacccgttta gaggccccaa ggggttatgc 120tagttattgc tcagcggtgg
cagcagccaa ctcagcttcc tttcgggctt tgttagcagc 180cggatcgatc
caagctgtac ctcactattc ctttgccctc ggacgagtgc tggggcgtcg
240gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
cgcttctgcg 300ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
acgattgcgt cgcatcgacc 360ctgcgcccaa gctgcatcat cgaaattgcc
gtcaaccaag ctctgataga gttggtcaag 420accaatgcgg agcatatacg
cccggagccg cggcgatcct gcaagctccg gatgcctccg 480ctcgaagtag
cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
540ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
ccgctgttat 600gcggccattg tccgtcagga cattgttgga gccgaaatcc
gcgtgcacga ggtgccggac 660ttcggggcag tcctcggccc aaagcatcag
ctcatcgaga gcctgcgcga cggacgcact 720gacggtgtcg tccatcacag
tttgccagtg atacacatgg ggatcagcaa tcgcgcatat 780gaaatcacgc
catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
840cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg
gctgcagaac 900agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
ccctgtgcac ggcgggagat 960gcaataggtc aggctctcgc tgaattcccc
aatgtcaagc acttccggaa tcgggagcgc 1020ggccgatgca aagtgccgat
aaacataacg atctttgtag aaaccatcgg cgcagctatt 1080tacccgcagg
acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
1140ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
acttctcgac 1200agacgtcgcg gtgagttcag gcttttccat gggtatatct
ccttcttaaa gttaaacaaa 1260attatttcta gagggaaacc gttgtggtct
ccctatagtg agtcgtatta atttcgcggg 1320atcgagatcg atccaattcc
aatcccacaa aaatctgagc ttaacagcac agttgctcct 1380ctcagagcag
aatcgggtat tcaacaccct catatcaact actacgttgt gtataacggt
1440ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa
cggcgttccc 1500ggagttgcac acaagaaatt tgccactatt acagaggcaa
gagcagcagc tgacgcgtac 1560acaacaagtc agcaaacaga caggttgaac
ttcatcccca aaggagaagc tcaactcaag 1620cccaagagct ttgctaaggc
cctaacaagc ccaccaaagc aaaaagccca ctggctcacg 1680ctaggaacca
aaaggcccag cagtgatcca gccccaaaag agatctcctt tgccccggag
1740attacaatgg acgatttcct ctatctttac gatctaggaa ggaagttcga
aggtgaaggt 1800gacgacacta tgttcaccac tgataatgag aaggttagcc
tcttcaattt cagaaagaat 1860gctgacccac agatggttag agaggcctac
gcagcaggtc tcatcaagac gatctacccg 1920agtaacaatc tccaggagat
caaatacctt cccaagaagg ttaaagatgc agtcaaaaga 1980ttcaggacta
attgcatcaa gaacacagag aaagacatat ttctcaagat cagaagtact
2040attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
agagattgga 2100gtctctaaaa aggtagttcc tactgaatct aaggccatgc
atggagtcta agattcaaat 2160cgaggatcta acagaactcg ccgtgaagac
tggcgaacag ttcatacaga gtcttttacg 2220actcaatgac aagaagaaaa
tcttcgtcaa catggtggag cacgacactc tggtctactc 2280caaaaatgtc
aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
2340gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
tcatcgaaag 2400gacagtagaa aaggaaggtg gctcctacaa atgccatcat
tgcgataaag gaaaggctat 2460cattcaagat gcctctgccg acagtggtcc
caaagatgga cccccaccca cgaggagcat 2520cgtggaaaaa gaagacgttc
caaccacgtc ttcaaagcaa gtggattgat gtgacatctc 2580cactgacgta
agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
2640aggaagttca tttcatttgg agaggacacg ctcgagctca tttctctatt
acttcagcca 2700taacaaaaga actcttttct cttcttatta aaccatgaaa
aagcctgaac tcaccgcgac 2760gtctgtcgag aagtttctga tcgaaaagtt
cgacagcgtc tccgacctga tgcagctctc 2820ggagggcgaa gaatctcgtg
ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 2880ggtaaatagc
tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
2940ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
gcctgaccta 3000ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
ctgcctgaaa ccgaactgcc 3060cgctgttctg cagccggtcg cggaggccat
ggatgcgatc gctgcggccg atcttagcca 3120gacgagcggg ttcggcccat
tcggaccgca aggaatcggt caatacacta catggcgtga 3180tttcatatgc
gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
3240cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
aggactgccc 3300cgaagtccgg cacctcgtgc acgcggattt cggctccaac
aatgtcctga cggacaatgg 3360ccgcataaca gcggtcattg actggagcga
ggcgatgttc ggggattccc aatacgaggt 3420cgccaacatc ttcttctgga
ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 3480cgagcggagg
catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
3540tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
cagcttgggc 3600gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
actgtcgggc gtacacaaat 3660cgcccgcaga agcgcggccg tctggaccga
tggctgtgta gaagtactcg ccgatagtgg 3720aaaccgacgc cccagcactc
gtccgagggc aaaggaatag tgaggtacct aaagaaggag 3780tgcgtcgaag
cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
3840gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
tgtaataatt 3900aacatgtaat gcatgacgtt atttatgaga tgggttttta
tgattagagt cccgcaatta 3960tacatttaat acgcgataga aaacaaaata
tagcgcgcaa actaggataa attatcgcgc 4020gcggtgtcat ctatgttact
agatcgatgt cgaatcgatc aacctgcatt aatgaatcgg 4080ccaacgcgcg
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
4140ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
aggcggtaat 4200acggttatcc acagaatcag gggataacgc aggaaagaac
atgtgagcaa aaggccagca 4260aaaggccagg aaccgtaaaa aggccgcgtt
gctggcgttt ttccataggc tccgcccccc 4320tgacgagcat cacaaaaatc
gacgctcaag tcagaggtgg cgaaacccga caggactata 4380aagataccag
gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
4440gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
ctcaatgctc 4500acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
aagctgggct gtgtgcacga 4560accccccgtt cagcccgacc gctgcgcctt
atccggtaac tatcgtcttg agtccaaccc 4620ggtaagacac gacttatcgc
cactggcagc agccactggt aacaggatta gcagagcgag 4680gtatgtaggc
ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4740gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
gagttggtag 4800ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
ttttttgttt gcaagcagca 4860gattacgcgc agaaaaaaag gatctcaaga
agatcctttg atcttttcta cggggtctga 4920cgctcagtgg aacgaaaact
cacgttaagg gattttggtc atgacattaa cctataaaaa 4980taggcgtatc
acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
5040acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
ggagcagaca 5100agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
ggctggctta actatgcggc 5160atcagagcag attgtactga gagtgcacca
tatggacata ttgtcgttag aacgcggcta 5220caattaatac ataaccttat
gtatcataca catacgattt aggtgacact atagaacggc 5280gcgccaagct
tggatctcct gcaggatata atgagccgta aacaaagatg attaagtagt
5340aattaatacg tactagtaaa agtggcaaaa gataacgaga aagaaccaat
ttctttgcat 5400tcggccttag cggaaggcat atataagctt tgattatttt
atttagtgta atgatttcgt 5460acaaccaaag catttattta gtactctcac
acttgtgtcg cggccgctca gtggaggagg 5520tgctcgaagg cgagagcctt
gatgttctgg tgcacgcact ggaagaggtt gccctcgcgg 5580tactcgaggc
catgcttctt gaagaagtcg cgtacctcgg gctggatagt cattaggttg
5640ccggtgggca tcatggggaa gaggtgatgc tcggtctgca gggagatgaa
gcccgtgagc 5700acgttcacta gccagccgcc cgtgatgtta cgggaggtct
tggcggtctg ctcgacgagc 5760gtcatgccct cgacgtgctc gcggtcgagg
atgtcctcac catagtgggt tgcaaagaca 5820acgatgcccg tcgcgaagcc
gcgtgcaacc atcatgagag ggatgaggtt gagatgcgct 5880gcgaagaccc
agcacaggag cgcgtagtga gcggcaagag cggcggcctg catccacatc
5940ttaggcaggc gcacagccac gtacgcgatg gactccaggc gccagtagag
gtcgaggatg 6000gccatggtcg gcacatagta gtactgctgc cagcgctgga
agaagttgag ccgcttggca 6060atggacgggt tctctcgcac gtagatgagc
aggggcgccg tcttgatgtc cgggtccgaa 6120ccatcctcgt tggtgcacac
gtggtgcgtg ttatggcgcg cgcgccacca ggccacgtcg 6180tagccctgga
ggaagccgag ggcgtatccc atggcgttgt tcgccttgac gagcgtgttg
6240accgagccct tgaagactgc gtgatgcagg tagtcgtgag caagccagcc
gtcgaggtag 6300aagccaaagc ccatggagag cgcgcccgcg aggggcgaga
cgccgctccg cacgagatag 6360gcgcccacga agatgggcgc aaggacgatg
gtgagcttga acagctcgtc caacgggcaa 6420gggtcgtaga gaccagccct
cttgtggcgc tcaatcatct ccttgaactc cgtgcccatg 6480gtggaccgcg
agggcagggg cacggaagcg ggcgcatcct ccgacttctt gagcatgccc
6540tccatcatct tgatggcctt gggcgagtgg taagactcga actgctccgt
cgcgtcgcgc 6600tcctggaaga tgcggaacac cttgctcccg cccgggtgtg
ccttctggaa ctttgtgaca 6660tccacgcgct tgccgtagag cacgacgtgc
acgctcttgc tatcgacgga gctcacctca 6720gcgagagacg cgtcggtccc
gctcaccgcc tgcgcgccgc cgtctccacc cttgcccatg 6780gtgcggccgc
ttggggggct atggaagact ttcttagtta gttgtgtgaa taagcaatgt
6840tgggagaatc gggactactt ataggatagg aataaaacag aaaagtatta
agtgctaatg 6900aaatatttag actgataatt aaaatcttca cgtatgtcca
cttgatataa aaacgtcagg 6960aataaaggaa gtacagtaga atttaaaggt
actcttttta tatatacccg tgttctcttt 7020ttggctagct agttgcataa
aaaataatct atatttttat cattatttta aatatcttat 7080gagatggtaa
atatttatca taattttttt tactattatt tattatttgt gtgtgtaata
7140catatagaag ttaattacaa attttattta ctttttcatt attttgatat
gattcaccat 7200taatttagtg ttattattta taatagttca ttttaatctt
tttgtatata ttatgcgtgc 7260agtacttttt tcctacatat aactactatt
acattttatt tatataatat ttttattaat 7320gaattttcgt gataatatgt
aatattgttc attattattt cagatttttt aaaaatattt 7380gtgttattat
ttatgaaata tgtaattttt ttagtatttg attttatgat gataaagtgt
7440tctaaattca aaagaagggg gaaagcgtaa acattaaaaa acgtcatcaa
acaaaaacaa 7500aatcttgtta ataaagataa aactgtttgt tttgatcact
gttatttcgt aatataaaaa 7560cattatttat atttatattg ttgacaacca
aatttgccta tcaaatctaa ccaatataat 7620gcatgcgtgg caggtaatgt
actaccatga acttaagtca tgacataata aaccgtgaat 7680ctgaccaatg
catgtaccta nctaaattgt atttgtgaca cgaagcaaat gattcaattc
7740acaatggaga tgggaaacaa ataatgaaga acccagaact aagaaagctt
ttctgaaaaa 7800taaaataaag gcaatgtcaa aagtatactg catcatcagt
ccagaaagca catgatattt 7860ttttatcagt atcaatgcag ctagttttat
tttacaatat cgatatagct agtttaaata 7920tattgcagct agatttataa
atatttgtgt tattatttat catttgtgta atcctgtttt 7980tagtatttta
gtttatatat gatgataatg tattccaaat ttaaaagaag ggaaataaat
8040ttaaacaaga aaaaaagtca tcaaacaaaa aacaaatgaa agggtggaaa
gatgttacca 8100tgtaatgtga atgttacagt atttctttta ttatagagtt
aacaaattaa ctaatatgat 8160tttgttaata atgataaaat atttttttta
ttattatttc ataatataaa aatagtttac 8220ttaatataaa aaaaattcta
tcgttcacaa caaagttggc cacctaattt aaccatgcat 8280gtacccatgg
accatattag gtaaccatca aacctgatga agagataaag agatgaagac
8340ttaagtcata acacaaaacc ataaaaaaca aaaatacaat caaccgtcaa
tctgaccaat 8400gcatgaaaaa gctgcaatag tgagtggcga cacaaagcac
atgattttct tacaacggag 8460ataaaaccaa aaaaatattt catgaacaac
ctagaacaaa taaagctttt atataataaa 8520tatataaata aataaaggct
atggaataat atacttcaat atatttggat taaataaatt 8580gttggcgggg
ttgatatatt tatacacacc taaagtcact tcaatctcat tttcacttaa
8640cttttatttt ttttttcttt ttatttatca taaagagaat attgataata
tactttttaa 8700catattttta tgacattttt tattggtgaa aacttattaa
aaatcataaa ttttgtaagt 8760tagatttatt taaagagttc ctcttcttat
tttaaatttt ttaataaatt tttaaataac 8820taaaatttgt gttaaaaatg
ttaaaaaatg tgttattaac ccttctcttc gaggacgtac 8880gtctagagtc gacctgca
8898267887DNAArtificial Sequenceplasmid pKR607 26ggccgcagta
tatcttaaat tctttaatac ggtgtactag gatattgaac tggttcttga 60tgatgaaaac
ctgggccgag attgcagcta tttatagtca taggtcttgt taacatgcat
120ggacatttgg ccacggggtg gcatgcagtt tgacgggtgt tgaaataaac
aaaaatgagg 180tggcggaaga gaatacgagt ttgaggttgg gttagaaaca
acaaatgtga gggctcatga 240tgggttgagt tggtgaatgt tttgggctgc
tcgattgaca cctttgtgag tacgtgttgt 300tgtgcatggc ttttggggtc
cagttttttt ttcttgacgc ggcgatcctg atcagctagt 360ggataagtga
tgtccactgt gtgtgattgc gtttttgttt gaattttatg aacttagaca
420ttgctatgca aaggatactc tcattgtgtt ttgtcttctt ttgttccttg
gctttttctt 480atgatccaag agactagtca gtgttgtggc attcgagact
accaagatta attatgatgg 540gggaaggata agtaactgat tagtacggac
tgttaccaaa ttaattaata agcggcaaat 600gaagggcatg gatcaaaagc
ttggatctcc tgcaggatct ggccggccgg atctcgtacg 660gatccgtcga
cggcgcgccc gatcatccgg atatagttcc tcctttcagc aaaaaacccc
720tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg
gtggcagcag 780ccaactcagc ttcctttcgg gctttgttag cagccggatc
gatccaagct gtacctcact 840attcctttgc cctcggacga gtgctggggc
gtcggtttcc actatcggcg agtacttcta 900cacagccatc ggtccagacg
gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc 960cggctccgga
tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
1020tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
tacgcccgga 1080gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
gtagcgcgtc tgctgctcca 1140tacaagccaa ccacggcctc cagaagaaga
tgttggcgac ctcgtattgg gaatccccga 1200acatcgcctc gctccagtca
atgaccgctg ttatgcggcc attgtccgtc aggacattgt 1260tggagccgaa
atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
1320tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
acagtttgcc 1380agtgatacac atggggatca gcaatcgcgc atatgaaatc
acgccatgta gtgtattgac 1440cgattccttg cggtccgaat gggccgaacc
cgctcgtctg gctaagatcg gccgcagcga 1500tcgcatccat agcctccgcg
accggctgca gaacagcggg cagttcggtt tcaggcaggt 1560cttgcaacgt
gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
1620ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
cgataaacat 1680aacgatcttt gtagaaacca tcggcgcagc tatttacccg
caggacatat ccacgccctc 1740ctacatcgaa gctgaaagca cgagattctt
cgccctccga gagctgcatc aggtcggaga 1800cgctgtcgaa cttttcgatc
agaaacttct cgacagacgt cgcggtgagt tcaggctttt 1860ccatgggtat
atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg
1920gtctccctat agtgagtcgt attaatttcg cgggatcgag atcgatccaa
ttccaatccc 1980acaaaaatct gagcttaaca gcacagttgc tcctctcaga
gcagaatcgg gtattcaaca 2040ccctcatatc aactactacg ttgtgtataa
cggtccacat gccggtatat acgatgactg 2100gggttgtaca aaggcggcaa
caaacggcgt tcccggagtt gcacacaaga aatttgccac 2160tattacagag
gcaagagcag cagctgacgc gtacacaaca agtcagcaaa cagacaggtt
2220gaacttcatc cccaaaggag aagctcaact caagcccaag agctttgcta
aggccctaac 2280aagcccacca aagcaaaaag cccactggct cacgctagga
accaaaaggc ccagcagtga 2340tccagcccca aaagagatct cctttgcccc
ggagattaca atggacgatt tcctctatct 2400ttacgatcta ggaaggaagt
tcgaaggtga aggtgacgac actatgttca ccactgataa 2460tgagaaggtt
agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc
2520ctacgcagca ggtctcatca agacgatcta cccgagtaac aatctccagg
agatcaaata 2580ccttcccaag aaggttaaag atgcagtcaa aagattcagg
actaattgca tcaagaacac 2640agagaaagac atatttctca agatcagaag
tactattcca gtatggacga ttcaaggctt 2700gcttcataaa ccaaggcaag
taatagagat tggagtctct aaaaaggtag ttcctactga 2760atctaaggcc
atgcatggag tctaagattc aaatcgagga tctaacagaa ctcgccgtga
2820agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag
aaaatcttcg 2880tcaacatggt ggagcacgac actctggtct actccaaaaa
tgtcaaagat acagtctcag 2940aagaccaaag ggctattgag acttttcaac
aaaggataat ttcgggaaac ctcctcggat 3000tccattgccc agctatctgt
cacttcatcg aaaggacagt agaaaaggaa ggtggctcct 3060acaaatgcca
tcattgcgat aaaggaaagg ctatcattca agatgcctct gccgacagtg
3120gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
gttccaacca 3180cgtcttcaaa gcaagtggat tgatgtgaca tctccactga
cgtaagggat gacgcacaat 3240cccactatcc ttcgcaagac ccttcctcta
tataaggaag ttcatttcat ttggagagga 3300cacgctcgag ctcatttctc
tattacttca gccataacaa aagaactctt ttctcttctt 3360attaaaccat
gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
3420agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
cgtgctttca 3480gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
tagctgcgcc gatggtttct 3540acaaagatcg ttatgtttat cggcactttg
catcggccgc gctcccgatt ccggaagtgc 3600ttgacattgg ggaattcagc
gagagcctga cctattgcat ctcccgccgt gcacagggtg 3660tcacgttgca
agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
3720ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
ccattcggac 3780cgcaaggaat cggtcaatac actacatggc gtgatttcat
atgcgcgatt gctgatcccc 3840atgtgtatca ctggcaaact gtgatggacg
acaccgtcag tgcgtccgtc gcgcaggctc 3900tcgatgagct gatgctttgg
gccgaggact gccccgaagt ccggcacctc gtgcacgcgg 3960atttcggctc
caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
4020gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
tggaggccgt 4080ggttggcttg tatggagcag cagacgcgct acttcgagcg
gaggcatccg gagcttgcag 4140gatcgccgcg gctccgggcg tatatgctcc
gcattggtct tgaccaactc tatcagagct 4200tggttgacgg caatttcgat
gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc 4260gatccggagc
cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
4320ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
actcgtccga 4380gggcaaagga atagtgaggt acctaaagaa ggagtgcgtc
gaagcagatc gttcaaacat 4440ttggcaataa agtttcttaa gattgaatcc
tgttgccggt cttgcgatga ttatcatata 4500atttctgttg aattacgtta
agcatgtaat aattaacatg taatgcatga cgttatttat 4560gagatgggtt
tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
4620aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
tactagatcg 4680atgtcgaatc gatcaacctg cattaatgaa tcggccaacg
cgcggggaga ggcggtttgc 4740gtattgggcg ctcttccgct tcctcgctca
ctgactcgct gcgctcggtc gttcggctgc 4800ggcgagcggt atcagctcac
tcaaaggcgg taatacggtt atccacagaa tcaggggata 4860acgcaggaaa
gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
4920cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
aatcgacgct 4980caagtcagag gtggcgaaac ccgacaggac tataaagata
ccaggcgttt ccccctggaa 5040gctccctcgt gcgctctcct gttccgaccc
tgccgcttac cggatacctg tccgcctttc 5100tcccttcggg aagcgtggcg
ctttctcaat gctcacgctg taggtatctc agttcggtgt 5160aggtcgttcg
ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
5220ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
tcgccactgg 5280cagcagccac tggtaacagg attagcagag cgaggtatgt
aggcggtgct acagagttct 5340tgaagtggtg gcctaactac ggctacacta
gaaggacagt atttggtatc tgcgctctgc 5400tgaagccagt taccttcgga
aaaagagttg gtagctcttg atccggcaaa caaaccaccg 5460ctggtagcgg
tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
5520aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt 5580aagggatttt ggtcatgaca ttaacctata aaaataggcg
tatcacgagg ccctttcgtc 5640tcgcgcgttt cggtgatgac ggtgaaaacc
tctgacacat gcagctcccg gagacggtca 5700cagcttgtct gtaagcggat
gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 5760ttggcgggtg
tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
5820accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
ttatgtatca 5880tacacatacg atttaggtga cactatagaa cggcgcgcca
agcttgttga aacatccctg 5940aagtgtctca ttttatttta tttattcttt
gctgataaaa aaataaaata aaagaagcta 6000agcacacggt caaccattgc
tctactgcta aaagggttat gtgtagtgtt ttactgcata 6060aattatgcag
caaacaagac aactcaaatt aaaaaatttc ctttgcttgt ttttttgttg
6120tctctgactt gactttcttg tggaagttgg ttgtataagg attgggacac
cattgtcctt 6180cttaatttaa ttttattctt tgctgataaa aaaaaaaatt
tcatatagtg ttaaataata 6240atttgttaaa taaccaaaaa gtcaaatatg
tttactctcg tttaaataat tgagattcgt 6300ccagcaaggc taaacgattg
tatagattta tgacaatatt tactttttta tagataaatg 6360ttatattata
ataaatttat atacatatat tatatgttat ttattattat tttaaatcct
6420tcaatatttt atcaaaccaa ctcataattt tttttttatc tgtaagaagc
aataaaatta 6480aatagaccca ctttaaggat gatccaacct ttatacagag
taagagagtt caaatagtac 6540cctttcatat acatatcaac taaaatatta
gaaatatcat ggatcaaacc ttataaagac 6600attaaataag tggataagta
taatatataa atgggtagta tataatatat aaatggatac 6660aaacttctct
ctttataatt gttatgtctc cttaacatcc taatataata cataagtggg
6720taatatataa tatataaatg gagacaaact tcttccatta taattgttat
gtcttcttaa 6780cacttatgtc tcgttcacaa tgctaaggtt agaattgttt
agaaagtctt atagtacaca 6840tttgtttttg tactatttga agcattccat
aagccgtcac gattcagatg atttataata 6900ataagaggaa atttatcata
gaacaataag gtgcatagat agagtgttaa tatatcataa 6960catcctttgt
ttattcatag aagaagtgag atggagctca gttattatac tgttacatgg
7020tcggatacaa tattccatgc tctccatgag ctcttacacc tacatgcatt
ttagttcata 7080cttgcggccg ctaaagctgc ttaccagcct tagcggattt
cttggtggcc aggttgtcct 7140ggtaaaagaa gtgacagaac aggagaaaga
cagatccgac gtaggcgtag ttgaaagccc 7200aggagaacag cttgcccttg
tcagagttga agcagggaac gttgatgtag tcccagacca 7260ggagaaagcc
accgacgaac tggcaaatct gcatggcagt gatcagaggc ttggccttga
7320acttgtagcc agcggcagtc agtccatagt aggtgtacat gatggtgtga
atgaacgagt 7380taaagaacat gaagatccac acaccctcgt tgtgcagtcg
aatgccgagg tagacgtccc 7440agggagctcc aaagtgatgg aaggcctgca
gaaaggacac tcgcttgccc ttgaggacca 7500gccaagcggt gtcgaggtac
tccacgtact tagaatagta gaaggccttg gcagtccagg 7560tgaacagctt
ggagtcccag acaggagagg gacactgaaa gagaggctgg ggagtatcac
7620cggtctgtct tcgcagccag gctccagtac cgtagtccca gccgagagcg
gtggcagtca 7680cgtagaagga cagggcagag aagagagcca ggaggacgtt
gtaccagatc atggaggttc 7740ggtaggctcc tttcttctcg tccacgagac
cagagtttcg caggagaggc ttcaggagca 7800ggtaggagaa ggtgccaatg
aggatttcgg gatcggtgac ggcagcccag attcgctcgc 7860cagcgtcgtt
ggccagagcc atggtgc 78872711482DNAArtificial Sequenceplasmid pKR903
27ggatctggcc ggccggatct cgtacggatc cgtcgacggc gcgcccgatc atccggatat
60agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
120tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
tgttagcagc 180cggatcgatc caagctgtac ctcactattc ctttgccctc
ggacgagtgc tggggcgtcg 240gtttccacta tcggcgagta cttctacaca
gccatcggtc cagacggccg cgcttctgcg 300ggcgatttgt gtacgcccga
cagtcccggc tccggatcgg acgattgcgt cgcatcgacc 360ctgcgcccaa
gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
420accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
gatgcctccg 480ctcgaagtag cgcgtctgct gctccataca agccaaccac
ggcctccaga agaagatgtt 540ggcgacctcg tattgggaat ccccgaacat
cgcctcgctc cagtcaatga ccgctgttat 600gcggccattg tccgtcagga
cattgttgga gccgaaatcc gcgtgcacga ggtgccggac 660ttcggggcag
tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
720gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
tcgcgcatat 780gaaatcacgc catgtagtgt attgaccgat tccttgcggt
ccgaatgggc cgaacccgct 840cgtctggcta agatcggccg cagcgatcgc
atccatagcc tccgcgaccg gctgcagaac 900agcgggcagt tcggtttcag
gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat 960gcaataggtc
aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
1020ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
cgcagctatt 1080tacccgcagg acatatccac gccctcctac atcgaagctg
aaagcacgag attcttcgcc 1140ctccgagagc tgcatcaggt cggagacgct
gtcgaacttt tcgatcagaa acttctcgac 1200agacgtcgcg gtgagttcag
gcttttccat gggtatatct ccttcttaaa gttaaacaaa 1260attatttcta
gagggaaacc gttgtggtct ccctatagtg agtcgtatta atttcgcggg
1320atcgagatcg atccaattcc aatcccacaa aaatctgagc ttaacagcac
agttgctcct 1380ctcagagcag aatcgggtat tcaacaccct catatcaact
actacgttgt gtataacggt 1440ccacatgccg gtatatacga tgactggggt
tgtacaaagg cggcaacaaa cggcgttccc 1500ggagttgcac acaagaaatt
tgccactatt acagaggcaa gagcagcagc tgacgcgtac 1560acaacaagtc
agcaaacaga caggttgaac ttcatcccca aaggagaagc tcaactcaag
1620cccaagagct ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca
ctggctcacg 1680ctaggaacca aaaggcccag cagtgatcca gccccaaaag
agatctcctt tgccccggag 1740attacaatgg acgatttcct ctatctttac
gatctaggaa ggaagttcga aggtgaaggt 1800gacgacacta tgttcaccac
tgataatgag aaggttagcc tcttcaattt cagaaagaat 1860gctgacccac
agatggttag agaggcctac gcagcaggtc tcatcaagac gatctacccg
1920agtaacaatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
agtcaaaaga 1980ttcaggacta attgcatcaa gaacacagag aaagacatat
ttctcaagat cagaagtact 2040attccagtat ggacgattca aggcttgctt
cataaaccaa ggcaagtaat agagattgga 2100gtctctaaaa aggtagttcc
tactgaatct aaggccatgc atggagtcta agattcaaat 2160cgaggatcta
acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
2220actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
tggtctactc 2280caaaaatgtc aaagatacag tctcagaaga ccaaagggct
attgagactt ttcaacaaag 2340gataatttcg ggaaacctcc tcggattcca
ttgcccagct atctgtcact tcatcgaaag 2400gacagtagaa aaggaaggtg
gctcctacaa atgccatcat tgcgataaag gaaaggctat 2460cattcaagat
gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
2520cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
gtgacatctc 2580cactgacgta agggatgacg cacaatccca ctatccttcg
caagaccctt cctctatata 2640aggaagttca tttcatttgg agaggacacg
ctcgagctca tttctctatt acttcagcca 2700taacaaaaga actcttttct
cttcttatta aaccatgaaa aagcctgaac tcaccgcgac 2760gtctgtcgag
aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
2820ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
atgtcctgcg 2880ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
gtttatcggc actttgcatc 2940ggccgcgctc ccgattccgg aagtgcttga
cattggggaa ttcagcgaga gcctgaccta 3000ttgcatctcc cgccgtgcac
agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 3060cgctgttctg
cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
3120gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
catggcgtga 3180tttcatatgc gcgattgctg atccccatgt gtatcactgg
caaactgtga tggacgacac 3240cgtcagtgcg tccgtcgcgc aggctctcga
tgagctgatg ctttgggccg aggactgccc 3300cgaagtccgg cacctcgtgc
acgcggattt cggctccaac aatgtcctga cggacaatgg 3360ccgcataaca
gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
3420cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
cgcgctactt 3480cgagcggagg catccggagc ttgcaggatc gccgcggctc
cgggcgtata tgctccgcat 3540tggtcttgac caactctatc agagcttggt
tgacggcaat ttcgatgatg cagcttgggc 3600gcagggtcga tgcgacgcaa
tcgtccgatc cggagccggg actgtcgggc gtacacaaat 3660cgcccgcaga
agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
3720aaaccgacgc cccagcactc gtccgagggc aaaggaatag tgaggtacct
aaagaaggag 3780tgcgtcgaag cagatcgttc aaacatttgg caataaagtt
tcttaagatt gaatcctgtt 3840gccggtcttg cgatgattat catataattt
ctgttgaatt acgttaagca tgtaataatt 3900aacatgtaat gcatgacgtt
atttatgaga tgggttttta tgattagagt cccgcaatta 3960tacatttaat
acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
4020gcggtgtcat ctatgttact agatcgatgt cgaatcgatc aacctgcatt
aatgaatcgg 4080ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
tccgcttcct cgctcactga 4140ctcgctgcgc tcggtcgttc ggctgcggcg
agcggtatca gctcactcaa aggcggtaat 4200acggttatcc acagaatcag
gggataacgc aggaaagaac atgtgagcaa aaggccagca 4260aaaggccagg
aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
4320tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
caggactata 4380aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
tctcctgttc cgaccctgcc 4440gcttaccgga tacctgtccg cctttctccc
ttcgggaagc gtggcgcttt ctcaatgctc 4500acgctgtagg tatctcagtt
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4560accccccgtt
cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
4620ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
gcagagcgag 4680gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
aactacggct acactagaag 4740gacagtattt ggtatctgcg ctctgctgaa
gccagttacc ttcggaaaaa gagttggtag 4800ctcttgatcc ggcaaacaaa
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4860gattacgcgc
agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4920cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgacattaa
cctataaaaa 4980taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
gatgacggtg aaaacctctg 5040acacatgcag ctcccggaga cggtcacagc
ttgtctgtaa gcggatgccg ggagcagaca 5100agcccgtcag ggcgcgtcag
cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 5160atcagagcag
attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta
5220caattaatac ataaccttat gtatcataca catacgattt aggtgacact
atagaacggc 5280gcgccaagct tgttgaaaca tccctgaagt gtctcatttt
attttattta ttctttgctg 5340ataaaaaaat aaaataaaag aagctaagca
cacggtcaac cattgctcta ctgctaaaag 5400ggttatgtgt agtgttttac
tgcataaatt atgcagcaaa caagacaact caaattaaaa 5460aatttccttt
gcttgttttt ttgttgtctc tgacttgact ttcttgtgga agttggttgt
5520ataaggattg ggacaccatt gtccttctta atttaatttt attctttgct
gataaaaaaa 5580aaaatttcat atagtgttaa ataataattt gttaaataac
caaaaagtca aatatgttta 5640ctctcgttta aataattgag attcgtccag
caaggctaaa cgattgtata gatttatgac 5700aatatttact tttttataga
taaatgttat attataataa atttatatac atatattata 5760tgttatttat
tattatttta aatccttcaa tattttatca aaccaactca taattttttt
5820tttatctgta agaagcaata aaattaaata gacccacttt aaggatgatc
caacctttat 5880acagagtaag agagttcaaa tagtaccctt tcatatacat
atcaactaaa atattagaaa 5940tatcatggat caaaccttat aaagacatta
aataagtgga taagtataat atataaatgg 6000gtagtatata atatataaat
ggatacaaac ttctctcttt ataattgtta tgtctcctta 6060acatcctaat
ataatacata agtgggtaat atataatata taaatggaga caaacttctt
6120ccattataat tgttatgtct tcttaacact tatgtctcgt tcacaatgct
aaggttagaa 6180ttgtttagaa agtcttatag tacacatttg tttttgtact
atttgaagca ttccataagc 6240cgtcacgatt cagatgattt ataataataa
gaggaaattt atcatagaac aataaggtgc 6300atagatagag tgttaatata
tcataacatc ctttgtttat tcatagaaga agtgagatgg 6360agctcagtta
ttatactgtt acatggtcgg atacaatatt ccatgctctc catgagctct
6420tacacctaca tgcattttag ttcatacttg cggccgctaa agctgcttac
cagccttagc 6480ggatttcttg gtggccaggt tgtcctggta aaagaagtga
cagaacagga gaaagacaga 6540tccgacgtag gcgtagttga aagcccagga
gaacagcttg cccttgtcag agttgaagca 6600gggaacgttg atgtagtccc
agaccaggag aaagccaccg acgaactggc aaatctgcat 6660ggcagtgatc
agaggcttgg ccttgaactt gtagccagcg gcagtcagtc catagtaggt
6720gtacatgatg gtgtgaatga acgagttaaa gaacatgaag atccacacac
cctcgttgtg 6780cagtcgaatg ccgaggtaga cgtcccaggg agctccaaag
tgatggaagg cctgcagaaa 6840ggacactcgc ttgcccttga ggaccagcca
agcggtgtcg aggtactcca cgtacttaga 6900atagtagaag gccttggcag
tccaggtgaa cagcttggag tcccagacag gagagggaca 6960ctgaaagaga
ggctggggag tatcaccggt ctgtcttcgc agccaggctc cagtaccgta
7020gtcccagccg agagcggtgg cagtcacgta gaaggacagg gcagagaaga
gagccaggag 7080gacgttgtac cagatcatgg aggttcggta ggctcctttc
ttctcgtcca cgagaccaga 7140gtttcgcagg agaggcttca ggagcaggta
ggagaaggtg ccaatgagga tttcgggatc 7200ggtgacggca gcccagattc
gctcgccagc gtcgttggcc agagccatgg tgcggccgca 7260gtatatctta
aattctttaa tacggtgtac taggatattg aactggttct tgatgatgaa
7320aacctgggcc gagattgcag ctatttatag tcataggtct tgttaacatg
catggacatt 7380tggccacggg gtggcatgca gtttgacggg tgttgaaata
aacaaaaatg aggtggcgga 7440agagaatacg agtttgaggt tgggttagaa
acaacaaatg tgagggctca tgatgggttg 7500agttggtgaa tgttttgggc
tgctcgattg acacctttgt gagtacgtgt tgttgtgcat 7560ggcttttggg
gtccagtttt tttttcttga cgcggcgatc ctgatcagct agtggataag
7620tgatgtccac tgtgtgtgat tgcgtttttg tttgaatttt atgaacttag
acattgctat 7680gcaaaggata ctctcattgt gttttgtctt cttttgttcc
ttggcttttt cttatgatcc 7740aagagactag tcagtgttgt ggcattcgag
actaccaaga ttaattatga tgggggaagg 7800ataagtaact gattagtacg
gactgttacc aaattaatta ataagcggca aatgaagggc 7860atggatcaaa
agcttggatc tcctgcaggt cgactctaga cgtacgtcct cgaagagaag
7920ggttaataac acatttttta acatttttaa cacaaatttt agttatttaa
aaatttatta 7980aaaaatttaa aataagaaga ggaactcttt aaataaatct
aacttacaaa atttatgatt 8040tttaataagt tttcaccaat aaaaaatgtc
ataaaaatat gttaaaaagt atattatcaa 8100tattctcttt atgataaata
aaaagaaaaa aaaaataaaa gttaagtgaa aatgagattg 8160aagtgacttt
aggtgtgtat aaatatatca accccgccaa caatttattt aatccaaata
8220tattgaagta tattattcca tagcctttat ttatttatat atttattata
taaaagcttt 8280atttgttcta ggttgttcat gaaatatttt tttggtttta
tctccgttgt aagaaaatca 8340tgtgctttgt gtcgccactc actattgcag
ctttttcatg cattggtcag attgacggtt 8400gattgtattt ttgtttttta
tggttttgtg ttatgactta agtcttcatc tctttatctc 8460ttcatcaggt
ttgatggtta cctaatatgg tccatgggta catgcatggt taaattaggt
8520ggccaacttt gttgtgaacg atagaatttt ttttatatta agtaaactat
ttttatatta 8580tgaaataata ataaaaaaaa tattttatca ttattaacaa
aatcatatta gttaatttgt 8640taactctata ataaaagaaa tactgtaaca
ttcacattac atggtaacat ctttccaccc 8700tttcatttgt tttttgtttg
atgacttttt ttcttgttta aatttatttc ccttctttta 8760aatttggaat
acattatcat catatataaa ctaaaatact aaaaacagga ttacacaaat
8820gataaataat aacacaaata tttataaatc tagctgcaat atatttaaac
tagctatatc 8880gatattgtaa aataaaacta gctgcattga tactgataaa
aaaatatcat gtgctttctg 8940gactgatgat gcagtatact tttgacattg
cctttatttt atttttcaga aaagctttct 9000tagttctggg ttcttcatta
tttgtttccc atctccattg tgaattgaat catttgcttc 9060gtgtcacaaa
tacaatttag ntaggtacat gcattggtca gattcacggt ttattatgtc
9120atgacttaag ttcatggtag tacattacct gccacgcatg cattatattg
gttagatttg 9180ataggcaaat ttggttgtca acaatataaa tataaataat
gtttttatat tacgaaataa 9240cagtgatcaa aacaaacagt tttatcttta
ttaacaagat tttgtttttg tttgatgacg 9300ttttttaatg tttacgcttt
cccccttctt ttgaatttag aacactttat catcataaaa 9360tcaaatacta
aaaaaattac atatttcata aataataaca caaatatttt taaaaaatct
9420gaaataataa tgaacaatat tacatattat cacgaaaatt cattaataaa
aatattatat 9480aaataaaatg taatagtagt tatatgtagg aaaaaagtac
tgcacgcata atatatacaa 9540aaagattaaa atgaactatt ataaataata
acactaaatt aatggtgaat catatcaaaa 9600taatgaaaaa gtaaataaaa
tttgtaatta acttctatat gtattacaca cacaaataat 9660aaataatagt
aaaaaaaatt atgataaata tttaccatct cataagatat ttaaaataat
9720gataaaaata tagattattt tttatgcaac tagctagcca aaaagagaac
acgggtatat 9780ataaaaagag tacctttaaa ttctactgta cttcctttat
tcctgacgtt tttatatcaa 9840gtggacatac gtgaagattt taattatcag
tctaaatatt tcattagcac ttaatacttt 9900tctgttttat tcctatccta
taagtagtcc cgattctccc aacattgctt attcacacaa 9960ctaactaaga
aagtcttcca tagcccccca agcggccgca ccatgggcaa gggtggagac
10020ggcggcgcgc aggcggtgag cgggaccgac gcgtctctcg ctgaggtgag
ctccgtcgat 10080agcaagagcg tgcacgtcgt gctctacggc aagcgcgtgg
atgtcacaaa gttccagaag 10140gcacacccgg gcgggagcaa ggtgttccgc
atcttccagg agcgcgacgc gacggagcag 10200ttcgagtctt accactcgcc
caaggccatc aagatgatgg agggcatgct caagaagtcg 10260gaggatgcgc
ccgcttccgt gcccctgccc tcgcggtcca ccatgggcac ggagttcaag
10320gagatgattg agcgccacaa gagggctggt ctctacgacc cttgcccgtt
ggacgagctg 10380ttcaagctca ccatcgtcct tgcgcccatc ttcgtgggcg
cctatctcgt gcggagcggc 10440gtctcgcccc tcgcgggcgc gctctccatg
ggctttggct tctacctcga cggctggctt 10500gctcacgact acctgcatca
cgcagtcttc aagggctcgg tcaacacgct cgtcaaggcg 10560aacaacgcca
tgggatacgc cctcggcttc ctccagggct acgacgtggc ctggtggcgc
10620gcgcgccata acacgcacca cgtgtgcacc aacgaggatg gttcggaccc
ggacatcaag 10680acggcgcccc tgctcatcta cgtgcgagag aacccgtcca
ttgccaagcg gctcaacttc 10740ttccagcgct ggcagcagta ctactatgtg
ccgaccatgg ccatcctcga cctctactgg 10800cgcctggagt ccatcgcgta
cgtggctgtg cgcctgccta agatgtggat gcaggccgcc 10860gctcttgccg
ctcactacgc gctcctgtgc tgggtcttcg cagcgcatct caacctcatc
10920cctctcatga tggttgcacg cggcttcgcg acgggcatcg ttgtctttgc
aacccactat 10980ggtgaggaca tcctcgaccg cgagcacgtc gagggcatga
cgctcgtcga gcagaccgcc 11040aagacctccc gtaacatcac gggcggctgg
ctagtgaacg tgctcacggg cttcatctcc 11100ctgcagaccg agcatcacct
cttccccatg atgcccaccg gcaacctaat gactatccag 11160cccgaggtac
gcgacttctt caagaagcat ggcctcgagt accgcgaggg caacctcttc
11220cagtgcgtgc accagaacat caaggctctc gccttcgagc acctcctcca
ctgagcggcc 11280gcgacacaag tgtgagagta ctaaataaat gctttggttg
tacgaaatca ttacactaaa 11340taaaataatc aaagcttata tatgccttcc
gctaaggccg aatgcaaaga aattggttct 11400ttctcgttat cttttgccac
ttttactagt acgtattaat tactacttaa tcatctttgt 11460ttacggctca
ttatatcctg ca 114822818DNAArtificial SequenceGPDsense primer
28atacgagatc gtcaaggg 182926DNAArtificial SequenceGPDantisense
primer 29gcggccgcgg attgatgtgt gtttaa 26306970DNAArtificial
Sequenceplasmid pY5-22GPD 30tcgacgcagt aggatgtcct gcacgggtct
ttttgtgggg tgtggagaaa ggggtgcttg 60gagatggaag ccggtagaac cgggctgctt
gtgcttggag atggaagccg gtagaaccgg 120gctgcttggg gggatttggg
gccgctgggc tccaaagagg ggtaggcatt tcgttggggt 180tacgtaattg
cggcatttgg gtcctgcgcg catgtcccat tggtcagaat tagtccggat
240aggagactta tcagccaatc acagcgccgg atccacctgt aggttgggtt
gggtgggagc 300acccctccac agagtagagt caaacagcag cagcaacatg
atagttgggg gtgtgcgtgt 360taaaggaaaa aaaagaagct tgggttatat
tcccgctcta tttagaggtt gcgggataga 420cgccgacgga gggcaatggc
gccatggaac cttgcggata tcgatacgcc gcggcggact 480gcgtccgaac
cagctccagc agcgtttttt ccgggccatt gagccgactg cgaccccgcc
540aacgtgtctt ggcccacgca
ctcatgtcat gttggtgttg ggaggccact ttttaagtag 600cacaaggcac
ctagctcgca gcaaggtgtc cgaaccaaag aagcggctgc agtggtgcaa
660acggggcgga aacggcggga aaaagccacg ggggcacgaa ttgaggcacg
ccctcgaatt 720tgagacgagt cacggcccca ttcgcccgcg caatggctcg
ccaacgcccg gtcttttgca 780ccacatcagg ttaccccaag ccaaaccttt
gtgttaaaaa gcttaacata ttataccgaa 840cgtaggtttg ggcgggcttg
ctccgtctgt ccaaggcaac atttatataa gggtctgcat 900cgccggctca
attgaatctt ttttcttctt ctcttctcta tattcattct tgaattaaac
960acacatcaat ccgcggccgc caccgcggcc cgagattccg gcctcttcgg
ccgccaagcg 1020acccgggtgg acgtctagag gtacctagca attaacagat
agtttgccgg tgataattct 1080cttaacctcc cacactcctt tgacataacg
atttatgtaa cgaaactgaa atttgaccag 1140atattgtgtc cgcggtggag
ctccagcttt tgttcccttt agtgagggtt aatttcgagc 1200ttggcgtaat
catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
1260cacaacgtac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
agtgagctaa 1320ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
cgggaaacct gtcgtgccag 1380ctgcattaat gaatcggcca acgcgcgggg
agaggcggtt tgcgtattgg gcgctcttcc 1440gcttcctcgc tcactgactc
gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 1500cactcaaagg
cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
1560tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
ggcgtttttc 1620cataggctcc gcccccctga cgagcatcac aaaaatcgac
gctcaagtca gaggtggcga 1680aacccgacag gactataaag ataccaggcg
tttccccctg gaagctccct cgtgcgctct 1740cctgttccga ccctgccgct
taccggatac ctgtccgcct ttctcccttc gggaagcgtg 1800gcgctttctc
atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
1860ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
cggtaactat 1920cgtcttgagt ccaacccggt aagacacgac ttatcgccac
tggcagcagc cactggtaac 1980aggattagca gagcgaggta tgtaggcggt
gctacagagt tcttgaagtg gtggcctaac 2040tacggctaca ctagaaggac
agtatttggt atctgcgctc tgctgaagcc agttaccttc 2100ggaaaaagag
ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
2160tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
tcctttgatc 2220ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
gttaagggat tttggtcatg 2280agattatcaa aaaggatctt cacctagatc
cttttaaatt aaaaatgaag ttttaaatca 2340atctaaagta tatatgagta
aacttggtct gacagttacc aatgcttaat cagtgaggca 2400cctatctcag
cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
2460ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
accgcgagac 2520ccacgctcac cggctccaga tttatcagca ataaaccagc
cagccggaag ggccgagcgc 2580agaagtggtc ctgcaacttt atccgcctcc
atccagtcta ttaattgttg ccgggaagct 2640agagtaagta gttcgccagt
taatagtttg cgcaacgttg ttgccattgc tacaggcatc 2700gtggtgtcac
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
2760cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
tcctccgatc 2820gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
ttatggcagc actgcataat 2880tctcttactg tcatgccatc cgtaagatgc
ttttctgtga ctggtgagta ctcaaccaag 2940tcattctgag aatagtgtat
gcggcgaccg agttgctctt gcccggcgtc aatacgggat 3000aataccgcgc
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
3060cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
cactcgtgca 3120cccaactgat cttcagcatc ttttactttc accagcgttt
ctgggtgagc aaaaacagga 3180aggcaaaatg ccgcaaaaaa gggaataagg
gcgacacgga aatgttgaat actcatactc 3240ttcctttttc aatattattg
aagcatttat cagggttatt gtctcatgag cggatacata 3300tttgaatgta
tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
3360ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
tacgcgcagc 3420gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt 3480ctcgccacgt tcgccggctt tccccgtcaa
gctctaaatc gggggctccc tttagggttc 3540cgatttagtg ctttacggca
cctcgacccc aaaaaacttg attagggtga tggttcacgt 3600agtgggccat
cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
3660aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
ctattctttt 3720gatttataag ggattttgcc gatttcggcc tattggttaa
aaaatgagct gatttaacaa 3780aaatttaacg cgaattttaa caaaatatta
acgcttacaa tttccattcg ccattcaggc 3840tgcgcaactg ttgggaaggg
cgatcggtgc gggcctcttc gctattacgc cagctggcga 3900aagggggatg
tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
3960gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
ttgggtaccg 4020ggccccccct cgaggtcgat ggtgtcgata agcttgatat
cgaattcatg tcacacaaac 4080cgatcttcgc ctcaaggaaa cctaattcta
catccgagag actgccgaga tccagtctac 4140actgattaat tttcgggcca
ataatttaaa aaaatcgtgt tatataatat tatatgtatt 4200atatatatac
atcatgatga tactgacagt catgtcccat tgctaaatag acagactcca
4260tctgccgcct ccaactgatg ttctcaatat ttaaggggtc atctcgcatt
gtttaataat 4320aaacagactc catctaccgc ctccaaatga tgttctcaaa
atatattgta tgaacttatt 4380tttattactt agtattatta gacaacttac
ttgctttatg aaaaacactt cctatttagg 4440aaacaattta taatggcagt
tcgttcattt aacaatttat gtagaataaa tgttataaat 4500gcgtatggga
aatcttaaat atggatagca taaatgatat ctgcattgcc taattcgaaa
4560tcaacagcaa cgaaaaaaat cccttgtaca acataaatag tcatcgagaa
atatcaacta 4620tcaaagaaca gctattcaca cgttactatt gagattatta
ttggacgaga atcacacact 4680caactgtctt tctctcttct agaaatacag
gtacaagtat gtactattct cattgttcat 4740acttctagtc atttcatccc
acatattcct tggatttctc tccaatgaat gacattctat 4800cttgcaaatt
caacaattat aataagatat accaaagtag cggtatagtg gcaatcaaaa
4860agcttctctg gtgtgcttct cgtatttatt tttattctaa tgatccatta
aaggtatata 4920tttatttctt gttatataat ccttttgttt attacatggg
ctggatacat aaaggtattt 4980tgatttaatt ttttgcttaa attcaatccc
ccctcgttca gtgtcaactg taatggtagg 5040aaattaccat acttttgaag
aagcaaaaaa aatgaaagaa aaaaaaaatc gtatttccag 5100gttagacgtt
ccgcagaatc tagaatgcgg tatgcggtac attgttcttc gaacgtaaaa
5160gttgcgctcc ctgagatatt gtacattttt gcttttacaa gtacaagtac
atcgtacaac 5220tatgtactac tgttgatgca tccacaacag tttgttttgt
ttttttttgt tttttttttt 5280tctaatgatt cattaccgct atgtatacct
acttgtactt gtagtaagcc gggttattgg 5340cgttcaatta atcatagact
tatgaatctg cacggtgtgc gctgcgagtt acttttagct 5400tatgcatgct
acttgggtgt aatattggga tctgttcgga aatcaacgga tgctcaaccg
5460atttcgacag taattaatta agtcatacac aagtcagctt tcttcgagcc
tcatataagt 5520ataagtagtt caacgtatta gcactgtacc cagcatctcc
gtatcgagaa acacaacaac 5580atgccccatt ggacagatca tgcggataca
caggttgtgc agtatcatac atactcgatc 5640agacaggtcg tctgaccatc
atacaagctg aacaagcgct ccatacttgc acgctctcta 5700tatacacagt
taaattacat atccatagtc taacctctaa cagttaatct tctggtaagc
5760ctcccagcca gccttctggt atcgcttggc ctcctcaata ggatctcggt
tctggccgta 5820cagacctcgg ccgacaatta tgatatccgt tccggtagac
atgacatcct caacagttcg 5880gtactgctgt ccgagagcgt ctcccttgtc
gtcaagaccc accccggggg tcagaataag 5940ccagtcctca gagtcgccct
taggtcggtt ctgggcaatg aagccaacca caaactcggg 6000gtcggatcgg
gcaagctcaa tggtctgctt ggagtactcg ccagtggcca gagagccctt
6060gcaagacagc tcggccagca tgagcagacc tctggccagc ttctcgttgg
gagaggggac 6120taggaactcc ttgtactggg agttctcgta gtcagagacg
tcctccttct tctgttcaga 6180gacagtttcc tcggcaccag ctcgcaggcc
agcaatgatt ccggttccgg gtacaccgtg 6240ggcgttggtg atatcggacc
actcggcgat tcggtgacac cggtactggt gcttgacagt 6300gttgccaata
tctgcgaact ttctgtcctc gaacaggaag aaaccgtgct taagagcaag
6360ttccttgagg gggagcacag tgccggcgta ggtgaagtcg tcaatgatgt
cgatatgggt 6420tttgatcatg cacacataag gtccgacctt atcggcaagc
tcaatgagct ccttggtggt 6480ggtaacatcc agagaagcac acaggttggt
tttcttggct gccacgagct tgagcactcg 6540agcggcaaag gcggacttgt
ggacgttagc tcgagcttcg taggagggca ttttggtggt 6600gaagaggaga
ctgaaataaa tttagtctgc agaacttttt atcggaacct tatctggggc
6660agtgaagtat atgttatggt aatagttacg agttagttga acttatagat
agactggact 6720atacggctat cggtccaaat tagaaagaac gtcaatggct
ctctgggcgt cgcctttgcc 6780gacaaaaatg tgatcatgat gaaagccagc
aatgacgttg cagctgatat tgttgtcggc 6840caaccgcgcc gaaaacgcag
ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa 6900agtgatccaa
gcacactcat agttggagtc gtactccaaa ggcggcaatg acgagtcaga
6960cagatactcg 6970318253DNAArtificial Sequenceplasmid pY118
31ggccgccacc gcggcccgag attccggcct cttcggccgc caagcgaccc gggtggacgt
60ctagaggtac ctagcaatta acagatagtt tgccggtgat aattctctta acctcccaca
120ctcctttgac ataacgattt atgtaacgaa actgaaattt gaccagatat
tgtgtccgcg 180gtggagctcc agcttttgtt ccctttagtg agggttaatt
tcgagcttgg cgtaatcatg 240gtcatagctg tttcctgtgt gaaattgtta
tccgctcaca attccacaca acgtacgagc 300cggaagcata aagtgtaaag
cctggggtgc ctaatgagtg agctaactca cattaattgc 360gttgcgctca
ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
420cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
cctcgctcac 480tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
tcagctcact caaaggcggt 540aatacggtta tccacagaat caggggataa
cgcaggaaag aacatgtgag caaaaggcca 600gcaaaaggcc aggaaccgta
aaaaggccgc gttgctggcg tttttccata ggctccgccc 660ccctgacgag
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
720ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
ttccgaccct 780gccgcttacc ggatacctgt ccgcctttct cccttcggga
agcgtggcgc tttctcatag 840ctcacgctgt aggtatctca gttcggtgta
ggtcgttcgc tccaagctgg gctgtgtgca 900cgaacccccc gttcagcccg
accgctgcgc cttatccggt aactatcgtc ttgagtccaa 960cccggtaaga
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
1020gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
gctacactag 1080aaggacagta tttggtatct gcgctctgct gaagccagtt
accttcggaa aaagagttgg 1140tagctcttga tccggcaaac aaaccaccgc
tggtagcggt ggtttttttg tttgcaagca 1200gcagattacg cgcagaaaaa
aaggatctca agaagatcct ttgatctttt ctacggggtc 1260tgacgctcag
tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
1320gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata 1380tgagtaaact tggtctgaca gttaccaatg cttaatcagt
gaggcaccta tctcagcgat 1440ctgtctattt cgttcatcca tagttgcctg
actccccgtc gtgtagataa ctacgatacg 1500ggagggctta ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc 1560tccagattta
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
1620aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
taagtagttc 1680gccagttaat agtttgcgca acgttgttgc cattgctaca
ggcatcgtgg tgtcacgctc 1740gtcgtttggt atggcttcat tcagctccgg
ttcccaacga tcaaggcgag ttacatgatc 1800ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 1860gttggccgca
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
1920gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
tctgagaata 1980gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
cgggataata ccgcgccaca 2040tagcagaact ttaaaagtgc tcatcattgg
aaaacgttct tcggggcgaa aactctcaag 2100gatcttaccg ctgttgagat
ccagttcgat gtaacccact cgtgcaccca actgatcttc 2160agcatctttt
actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
2220aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
tttttcaata 2280ttattgaagc atttatcagg gttattgtct catgagcgga
tacatatttg aatgtattta 2340gaaaaataaa caaatagggg ttccgcgcac
atttccccga aaagtgccac ctgacgcgcc 2400ctgtagcggc gcattaagcg
cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 2460tgccagcgcc
ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
2520cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
ttagtgcttt 2580acggcacctc gaccccaaaa aacttgatta gggtgatggt
tcacgtagtg ggccatcgcc 2640ctgatagacg gtttttcgcc ctttgacgtt
ggagtccacg ttctttaata gtggactctt 2700gttccaaact ggaacaacac
tcaaccctat ctcggtctat tcttttgatt tataagggat 2760tttgccgatt
tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
2820ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
caactgttgg 2880gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
tggcgaaagg gggatgtgct 2940gcaaggcgat taagttgggt aacgccaggg
ttttcccagt cacgacgttg taaaacgacg 3000gccagtgaat tgtaatacga
ctcactatag ggcgaattgg gtaccgggcc ccccctcgag 3060gtcgatggtg
tcgataagct tgatatcgaa ttcatgtcac acaaaccgat cttcgcctca
3120aggaaaccta attctacatc cgagagactg ccgagatcca gtctacactg
attaattttc 3180gggccaataa tttaaaaaaa tcgtgttata taatattata
tgtattatat atatacatca 3240tgatgatact gacagtcatg tcccattgct
aaatagacag actccatctg ccgcctccaa 3300ctgatgttct caatatttaa
ggggtcatct cgcattgttt aataataaac agactccatc 3360taccgcctcc
aaatgatgtt ctcaaaatat attgtatgaa cttattttta ttacttagta
3420ttattagaca acttacttgc tttatgaaaa acacttccta tttaggaaac
aatttataat 3480ggcagttcgt tcatttaaca atttatgtag aataaatgtt
ataaatgcgt atgggaaatc 3540ttaaatatgg atagcataaa tgatatctgc
attgcctaat tcgaaatcaa cagcaacgaa 3600aaaaatccct tgtacaacat
aaatagtcat cgagaaatat caactatcaa agaacagcta 3660ttcacacgtt
actattgaga ttattattgg acgagaatca cacactcaac tgtctttctc
3720tcttctagaa atacaggtac aagtatgtac tattctcatt gttcatactt
ctagtcattt 3780catcccacat attccttgga tttctctcca atgaatgaca
ttctatcttg caaattcaac 3840aattataata agatatacca aagtagcggt
atagtggcaa tcaaaaagct tctctggtgt 3900gcttctcgta tttattttta
ttctaatgat ccattaaagg tatatattta tttcttgtta 3960tataatcctt
ttgtttatta catgggctgg atacataaag gtattttgat ttaatttttt
4020gcttaaattc aatcccccct cgttcagtgt caactgtaat ggtaggaaat
taccatactt 4080ttgaagaagc aaaaaaaatg aaagaaaaaa aaaatcgtat
ttccaggtta gacgttccgc 4140agaatctaga atgcggtatg cggtacattg
ttcttcgaac gtaaaagttg cgctccctga 4200gatattgtac atttttgctt
ttacaagtac aagtacatcg tacaactatg tactactgtt 4260gatgcatcca
caacagtttg ttttgttttt ttttgttttt tttttttcta atgattcatt
4320accgctatgt atacctactt gtacttgtag taagccgggt tattggcgtt
caattaatca 4380tagacttatg aatctgcacg gtgtgcgctg cgagttactt
ttagcttatg catgctactt 4440gggtgtaata ttgggatctg ttcggaaatc
aacggatgct caaccgattt cgacagtaat 4500taattaagtc atacacaagt
cagctttctt cgagcctcat ataagtataa gtagttcaac 4560gtattagcac
tgtacccagc atctccgtat cgagaaacac aacaacatgc cccattggac
4620agatcatgcg gatacacagg ttgtgcagta tcatacatac tcgatcagac
aggtcgtctg 4680accatcatac aagctgaaca agcgctccat acttgcacgc
tctctatata cacagttaaa 4740ttacatatcc atagtctaac ctctaacagt
taatcttctg gtaagcctcc cagccagcct 4800tctggtatcg cttggcctcc
tcaataggat ctcggttctg gccgtacaga cctcggccga 4860caattatgat
atccgttccg gtagacatga catcctcaac agttcggtac tgctgtccga
4920gagcgtctcc cttgtcgtca agacccaccc cgggggtcag aataagccag
tcctcagagt 4980cgcccttagg tcggttctgg gcaatgaagc caaccacaaa
ctcggggtcg gatcgggcaa 5040gctcaatggt ctgcttggag tactcgccag
tggccagaga gcccttgcaa gacagctcgg 5100ccagcatgag cagacctctg
gccagcttct cgttgggaga ggggactagg aactccttgt 5160actgggagtt
ctcgtagtca gagacgtcct ccttcttctg ttcagagaca gtttcctcgg
5220caccagctcg caggccagca atgattccgg ttccgggtac accgtgggcg
ttggtgatat 5280cggaccactc ggcgattcgg tgacaccggt actggtgctt
gacagtgttg ccaatatctg 5340cgaactttct gtcctcgaac aggaagaaac
cgtgcttaag agcaagttcc ttgaggggga 5400gcacagtgcc ggcgtaggtg
aagtcgtcaa tgatgtcgat atgggttttg atcatgcaca 5460cataaggtcc
gaccttatcg gcaagctcaa tgagctcctt ggtggtggta acatccagag
5520aagcacacag gttggttttc ttggctgcca cgagcttgag cactcgagcg
gcaaaggcgg 5580acttgtggac gttagctcga gcttcgtagg agggcatttt
ggtggtgaag aggagactga 5640aataaattta gtctgcagaa ctttttatcg
gaaccttatc tggggcagtg aagtatatgt 5700tatggtaata gttacgagtt
agttgaactt atagatagac tggactatac ggctatcggt 5760ccaaattaga
aagaacgtca atggctctct gggcgtcgcc tttgccgaca aaaatgtgat
5820catgatgaaa gccagcaatg acgttgcagc tgatattgtt gtcggccaac
cgcgccgaaa 5880acgcagctgt cagacccaca gcctccaacg aagaatgtat
cgtcaaagtg atccaagcac 5940actcatagtt ggagtcgtac tccaaaggcg
gcaatgacga gtcagacaga tactcgtcga 6000cgcagtagga tgtcctgcac
gggtcttttt gtggggtgtg gagaaagggg tgcttggaga 6060tggaagccgg
tagaaccggg ctgcttgtgc ttggagatgg aagccggtag aaccgggctg
6120cttgggggga tttggggccg ctgggctcca aagaggggta ggcatttcgt
tggggttacg 6180taattgcggc atttgggtcc tgcgcgcatg tcccattggt
cagaattagt ccggatagga 6240gacttatcag ccaatcacag cgccggatcc
acctgtaggt tgggttgggt gggagcaccc 6300ctccacagag tagagtcaaa
cagcagcagc aacatgatag ttgggggtgt gcgtgttaaa 6360ggaaaaaaaa
gaagcttggg ttatattccc gctctattta gaggttgcgg gatagacgcc
6420gacggagggc aatggcgcca tggaaccttg cggatatcga tacgccgcgg
cggactgcgt 6480ccgaaccagc tccagcagcg ttttttccgg gccattgagc
cgactgcgac cccgccaacg 6540tgtcttggcc cacgcactca tgtcatgttg
gtgttgggag gccacttttt aagtagcaca 6600aggcacctag ctcgcagcaa
ggtgtccgaa ccaaagaagc ggctgcagtg gtgcaaacgg 6660ggcggaaacg
gcgggaaaaa gccacggggg cacgaattga ggcacgccct cgaatttgag
6720acgagtcacg gccccattcg cccgcgcaat ggctcgccaa cgcccggtct
tttgcaccac 6780atcaggttac cccaagccaa acctttgtgt taaaaagctt
aacatattat accgaacgta 6840ggtttgggcg ggcttgctcc gtctgtccaa
ggcaacattt atataagggt ctgcatcgcc 6900ggctcaattg aatctttttt
cttcttctct tctctatatt cattcttgaa ttaaacacac 6960atcaatccgc
ggccgcacca tgggcaaggg tggagacggc ggcgcgcagg cggtgagcgg
7020gaccgacgcg tctctcgctg aggtgagctc cgtcgatagc aagagcgtgc
acgtcgtgct 7080ctacggcaag cgcgtggatg tcacaaagtt ccagaaggca
cacccgggcg ggagcaaggt 7140gttccgcatc ttccaggagc gcgacgcgac
ggagcagttc gagtcttacc actcgcccaa 7200ggccatcaag atgatggagg
gcatgctcaa gaagtcggag gatgcgcccg cttccgtgcc 7260cctgccctcg
cggtccacca tgggcacgga gttcaaggag atgattgagc gccacaagag
7320ggctggtctc tacgaccctt gcccgttgga cgagctgttc aagctcacca
tcgtccttgc 7380gcccatcttc gtgggcgcct atctcgtgcg gagcggcgtc
tcgcccctcg cgggcgcgct 7440ctccatgggc tttggcttct acctcgacgg
ctggcttgct cacgactacc tgcatcacgc 7500agtcttcaag ggctcggtca
acacgctcgt caaggcgaac aacgccatgg gatacgccct 7560cggcttcctc
cagggctacg acgtggcctg gtggcgcgcg cgccataaca cgcaccacgt
7620gtgcaccaac gaggatggtt cggacccgga catcaagacg gcgcccctgc
tcatctacgt 7680gcgagagaac ccgtccattg ccaagcggct caacttcttc
cagcgctggc agcagtacta 7740ctatgtgccg accatggcca tcctcgacct
ctactggcgc ctggagtcca tcgcgtacgt 7800ggctgtgcgc ctgcctaaga
tgtggatgca ggccgccgct cttgccgctc actacgcgct 7860cctgtgctgg
gtcttcgcag cgcatctcaa cctcatccct ctcatgatgg ttgcacgcgg
7920cttcgcgacg ggcatcgttg tctttgcaac ccactatggt gaggacatcc
tcgaccgcga 7980gcacgtcgag ggcatgacgc tcgtcgagca gaccgccaag
acctcccgta acatcacggg 8040cggctggcta gtgaacgtgc tcacgggctt
catctccctg cagaccgagc atcacctctt 8100ccccatgatg cccaccggca
acctaatgac tatccagccc gaggtacgcg acttcttcaa 8160gaagcatggc
ctcgagtacc gcgagggcaa cctcttccag tgcgtgcacc agaacatcaa
8220ggctctcgcc ttcgagcacc tcctccactg agc 825332777DNAEuglena
gracilis 32atggaggtgg tgaatgaaat agtctcaatt gggcaggaag ttttacccaa
agttgattat 60gcccaactct ggagtgatgc cagtcactgt gaggtgcttt acttgtccat
cgcatttgtc 120atcttgaagt tcactcttgg cccccttggt ccaaaaggtc
agtctcgtat gaagtttgtt 180ttcaccaatt acaaccttct catgtccatt
tattcgttgg gatcattcct ctcaatggca 240tatgccatgt
acaccatcgg tgttatgtct gacaactgcg agaaggcttt tgacaacaac
300gtcttcagga tcaccacgca gttgttctat ttgagcaagt tcctggagta
tattgactcc 360ttctatttgc cactgatggg caagcctctg acctggttgc
aattcttcca tcatttgggg 420gcaccgatgg atatgtggct gttctataat
taccgaaatg aagctgtttg gatttttgtg 480ctgttgaatg gtttcatcca
ctggatcatg tacggttatt attggaccag attgatcaag 540ctgaagttcc
ccatgccaaa atccctgatt acatcaatgc agatcattca attcaatgtt
600ggtttctaca ttgtctggaa gtacaggaac attccctgtt atcgccaaga
tgggatgagg 660atgtttggct ggttcttcaa ttacttttat gttggcacag
tcttgtgttt gttcttgaat 720ttctatgtgc aaacgtatat cgtcaggaag
cacaagggag ccaaaaagat tcagtga 7773330DNAArtificial Sequencesense
oligonucleotide oEugEL1-1 primer 33agcggccgca ccatggaggt ggtgaatgaa
303430DNAArtificial Sequencesense oligonucleotide oEugEL1-2 primer
34tgcggccgct cactgaatct ttttggctcc 30354311DNAArtificial
Sequenceplasmid pKR906 35agcggccgca ccatggaggt ggtgaatgaa
atagtctcaa ttgggcagga agttttaccc 60aaagttgatt atgcccaact ctggagtgat
gccagtcact gtgaggtgct ttacttgtcc 120atcgcatttg tcatcttgaa
gttcactctt ggcccccttg gtccaaaagg tcagtctcgt 180atgaagtttg
ttttcaccaa ttacaacctt ctcatgtcca tttattcgtt gggatcattc
240ctctcaatgg catatgccat gtacaccatc ggtgttatgt ctgacaactg
cgagaaggct 300tttgacaaca acgtcttcag gatcaccacg cagttgttct
atttgagcaa gttcctggag 360tatattgact ccttctattt gccactgatg
ggcaagcctc tgacctggtt gcaattcttc 420catcatttgg gggcaccgat
ggatatgtgg ctgttctata attaccgaaa tgaagctgtt 480tggatttttg
tgctgttgaa tggtttcatc cactggatca tgtacggtta ttattggacc
540agattgatca agctgaagtt ccccatgcca aaatccctga ttacatcaat
gcagatcatt 600caattcaatg ttggtttcta cattgtctgg aagtacagga
acattccctg ttatcgccaa 660gatgggatga ggatgtttgg ctggttcttc
aattactttt atgttggcac agtcttgtgt 720ttgttcttga atttctatgt
gcaaacgtat atcgtcagga agcacaaggg agccaaaaag 780attcagtgag
cggccgcacc tgaattccag cacactggcg gccgttacta gtggatccga
840gctcggtacc aagcttgatg catagcttga gtattctaac gcgtcaccta
aatagcttgg 900cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
tccgctcaca attccacaca 960acatacgagc cggaagcata aagtgtaaag
cctggggtgc ctaatgagtg agctaactca 1020cattaattgc gttgcgctca
ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 1080attaatgaat
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
1140cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
tcagctcact 1200caaaggcggt aatacggtta tccacagaat caggggataa
cgcaggaaag aacatgtgag 1260caaaaggcca gcaaaagccc aggaaccgta
aaaaggccgc gttgctggcg tttttccata 1320ggctccgccc ccctgacgag
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 1380cgacaggact
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
1440ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
agcgtggcgc 1500tttctcatag ctcacgctgt aggtatctca gttcggtgta
ggtcgttcgc tccaagctgg 1560gctgtgtgca cgaacccccc gttcagcccg
accgctgcgc cttatccggt aactatcgtc 1620ttgagtccaa cccggtaaga
cacgacttat cgccactggc agcagccact ggtaacagga 1680ttagcagagc
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
1740gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
accttcggaa 1800aaagagttgg tagctcttga tccggcaaac aaaccaccgc
tggtagcggt ggtttttttg 1860tttgcaagca gcagattacg cgcagaaaaa
aaggatctca agaagatcct ttgatctttt 1920ctacggggtc tgacgctcag
tggaacgaaa actcacgtta agggattttg gtcatgagat 1980tatcaaaaag
gatcttcacc tagatccttt taaattaaaa atgaagtttt agcacgtgtc
2040agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc
agggcgaact 2100cccgccccca cggctgctcg ccgatctcgg tcatggccgg
cccggaggcg tcccggaagt 2160tcgtggacac gacctccgac cactcggcgt
acagctcgtc caggccgcgc acccacaccc 2220aggccagggt gttgtccggc
accacctggt cctggaccgc gctgatgaac agggtcacgt 2280cgtcccggac
cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
2340cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga
acggcactgg 2400tcaacttggc catggtggcc ctcctcacgt gctattattg
aagcatttat cagggttatt 2460gtctcatgag cggatacata tttgaatgta
tttagaaaaa taaacaaata ggggttccgc 2520gcacatttcc ccgaaaagtg
ccacctgtat gcggtgtgaa ataccgcaca gatgcgtaag 2580gagaaaatac
cgcatcagga aattgtaagc gttaataatt cagaagaact cgtcaagaag
2640gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
cgaggaagcg 2700gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
gtagccaacg ctatgtcctg 2760atagcggtcc gccacaccca gccggccaca
gtcgatgaat ccagaaaagc ggccattttc 2820caccatgata ttcggcaagc
aggcatcgcc atgggtcacg acgagatcct cgccgtcggg 2880catgctcgcc
ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
2940cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
cgatgcgatg 3000tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
gtatgcagcc gccgcattgc 3060atcagccatg atggatactt tctcggcagg
agcaaggtga gatgacagga gatcctgccc 3120cggcacttcg cccaatagca
gccagtccct tcccgcttca gtgacaacgt cgagcacagc 3180tgcgcaagga
acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
3240attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
gcgctgacag 3300ccggaacacg gcggcatcag agcagccgat tgtctgttgt
gcccagtcat agccgaatag 3360cctctccacc caagcggccg gagaacctgc
gtgcaatcca tcttgttcaa tcatgcgaaa 3420cgatcctcat cctgtctctt
gatcagagct tgatcccctg cgccatcaga tccttggcgg 3480cgagaaagcc
atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
3540tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
gccatgtaag 3600cccactgcaa gctacctgct ttctctttgc gcttgcgttt
tcccttgtcc agatagccca 3660gtagctgaca ttcatccggg gtcagcaccg
tttctgcgga ctggctttct acgtgaaaag 3720gatctaggtg aagatccttt
ttgataatct catgcctgac atttatattc cccagaacat 3780caggttaatg
gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
3840gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
tttcatcccc 3900gatatgcacc accgggtaaa gttcacggga gactttatct
gacagcagac gtgcactggc 3960cagggggatc accatccgtc gccccggcgt
gtcaataata tcactctgta catccacaaa 4020cagacgataa cggctctctc
ttttataggt gtaaacctta aactgccgta cgtataggct 4080gcgcaactgt
tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
4140agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
agtcacgacg 4200ttgtaaaacg acggccagtg aattgtaata cgactcacta
tagggcgaat tgggccctct 4260agatgcatgc tcgagcggcc gccagtgtga
tggatatctg cagaattcag g 4311363983DNAArtificial Sequenceplasmid
pKR132 36ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct
gtttcctgtg 60tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
aaagtgtaaa 120gcctggggtg cctaatgagt gagctaactc acattaattg
cgttgcgctc actgcccgct 180ttccagtcgg gaaacctgtc gtgccagctg
cattaatgaa tcggccaacg cgcggggaga 240ggcggtttgc gtattgggcg
ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 300gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
360tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
caggaaccgt 420aaaaaggccg cgttgctggc gtttttccat aggctccgcc
cccctgacga gcatcacaaa 480aatcgacgct caagtcagag gtggcgaaac
ccgacaggac tataaagata ccaggcgttt 540ccccctggaa gctccctcgt
gcgctctcct gttccgaccc tgccgcttac cggatacctg 600tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
660agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
cgttcagccc 720gaccgctgcg ccttatccgg taactatcgt cttgagtcca
acccggtaag acacgactta 780tcgccactgg cagcagccac tggtaacagg
attagcagag cgaggtatgt aggcggtgct 840acagagttct tgaagtggtg
gcctaactac ggctacacta gaaggacagt atttggtatc 900tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
960caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
gcgcagaaaa 1020aaaggatctc aagaagatcc tttgatcttt tctacggggt
ctgacgctca gtggaacgaa 1080aactcacgtt aagggatttt ggtcatgaga
ttatcaaaaa ggatcttcac ctagatcctt 1140ttaaattaaa aatgaagttt
taaatcaatc taaagtatat atgagtaaac ttggtctgac 1200agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
1260atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
accatctggc 1320cccagtgctg caatgatacc gcgagaccca cgctcaccgg
ctccagattt atcagcaata 1380aaccagccag ccggaagggc cgagcgcaga
agtggtcctg caactttatc cgcctccatc 1440cagtctatta attgttgccg
ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 1500aacgttgttg
ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
1560ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
gtgcaaaaaa 1620gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
agttggccgc agtgttatca 1680ctcatggtta tggcagcact gcataattct
cttactgtca tgccatccgt aagatgcttt 1740tctgtgactg gtgagtactc
aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 1800tgctcttgcc
cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
1860ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
gctgttgaga 1920tccagttcga tgtaacccac tcgtgcaccc aactgatctt
cagcatcttt tactttcacc 1980agcgtttctg ggtgagcaaa aacaggaagg
caaaatgccg caaaaaaggg aataagggcg 2040acacggaaat gttgaatact
catactcttc ctttttcaat attattgaag catttatcag 2100ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
2160gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
tattatcatg 2220acattaacct ataaaaatag gcgtatcacg aggccctttc
gtctcgcgcg tttcggtgat 2280gacggtgaaa acctctgaca catgcagctc
ccggagacgg tcacagcttg tctgtaagcg 2340gatgccggga gcagacaagc
ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 2400tggcttaact
atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
2460ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
attcaggctg 2520cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
tattacgcca gctggcgaaa 2580gggggatgtg ctgcaaggcg attaagttgg
gtaacgccag ggttttccca gtcacgacgt 2640tgtaaaacga cggccagtga
attcgagctc ggtacccggg gatcctctag acctgcaggc 2700caactgcgtt
tggggctcca gattaaacga cgccgtttcg ttcctttcgc ttcacggctt
2760aacgatgtcg tttctgtctg tgcccaaaaa ataaaggcat ttgttatttg
caccagatat 2820ttactaagtg caccctagtt tgacaagtag gcgataatta
caaatagatg cggtgcaaat 2880aataaatttt gaaggaaata attacaaaag
aacagaactt atatttactt tattttaaaa 2940aactaaaatg aaagaacaaa
aaaagtaaaa aatacaaaaa atgtgcttta accactttca 3000ttatttgtta
cagaaagtat gattctactc aaattgatct gttgtatctg gtgctgcctt
3060gtcacactgg cgatttcaat cccctaaaga tatggtgcaa actgcgaagt
gatcaatatc 3120tgctcggtta atttagatta attaataata ttcaacgtga
tgtaccaaaa aaagacaatt 3180ttttgctcca ttgacaaatt aaacctcatc
aaggtaattt ccaaacctat aagcaaaaaa 3240atttcacatt aattggcccg
caatcctatt agtcttatta tactagagta ggaaaaaaaa 3300caattacaca
acttgtctta ttattctcta tgctaatgaa tatttttccc ttttgttaga
3360aatcagtgtt tcctaattta ttgagtatta attccactca ccgcatatat
ttaccgttga 3420ataagaaaat tttacacata attcttttta agataaataa
tttttttata ctagatctta 3480tatgattacg tgaagccaag tgggttatac
taatgatata taatgtttga tagtaatcag 3540tttataaacc aaatgcatgg
aaatgttacg tggaagcacg taaattaaca agcattgaag 3600caaatgcagc
caccgcacca aaaccacccc acttcacttc cacgtaccat attccatgca
3660actacaacac cctaaaactt caataaatgc ccccaccttc acttcacttc
acccatcaat 3720agcaagcggc cgcgaagtta aaagcaatgt tgtcacttgt
cgtactaaca catgatgtga 3780tagtttatgc tagctagcta taacataagc
tgtctctgag tgtgttgtat attaataaag 3840atcatcactg gtgaatggtg
atcgtgtacg taccctactt agtaggcaat ggaagcactt 3900agagtgtgct
ttgtgcatgg ccttgcctct gttttgagac ttttgtaatg ttttcgagtt
3960taaatctttg cctttgcgta cgt 3983374771DNAArtificial
Sequenceplasmid pKR953 37ggccgcgaag ttaaaagcaa tgttgtcact
tgtcgtacta acacatgatg tgatagttta 60tgctagctag ctataacata agctgtctct
gagtgtgttg tatattaata aagatcatca 120ctggtgaatg gtgatcgtgt
acgtacccta cttagtaggc aatggaagca cttagagtgt 180gctttgtgca
tggccttgcc tctgttttga gacttttgta atgttttcga gtttaaatct
240ttgcctttgc gtacgtctag agtcgacctg caggcatgca agcttggcgt
aatcatggtc 300atagctgttt cctgtgtgaa attgttatcc gctcacaatt
ccacacaaca tacgagccgg 360aagcataaag tgtaaagcct ggggtgccta
atgagtgagc taactcacat taattgcgtt 420gcgctcactg cccgctttcc
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 480ccaacgcgcg
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
540ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
aggcggtaat 600acggttatcc acagaatcag gggataacgc aggaaagaac
atgtgagcaa aaggccagca 660aaaggccagg aaccgtaaaa aggccgcgtt
gctggcgttt ttccataggc tccgcccccc 720tgacgagcat cacaaaaatc
gacgctcaag tcagaggtgg cgaaacccga caggactata 780aagataccag
gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
840gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
ctcatagctc 900acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
aagctgggct gtgtgcacga 960accccccgtt cagcccgacc gctgcgcctt
atccggtaac tatcgtcttg agtccaaccc 1020ggtaagacac gacttatcgc
cactggcagc agccactggt aacaggatta gcagagcgag 1080gtatgtaggc
ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
1140gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
gagttggtag 1200ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
ttttttgttt gcaagcagca 1260gattacgcgc agaaaaaaag gatctcaaga
agatcctttg atcttttcta cggggtctga 1320cgctcagtgg aacgaaaact
cacgttaagg gattttggtc atgagattat caaaaaggat 1380cttcacctag
atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
1440gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
cagcgatctg 1500tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
tagataacta cgatacggga 1560gggcttacca tctggcccca gtgctgcaat
gataccgcga gacccacgct caccggctcc 1620agatttatca gcaataaacc
agccagccgg aagggccgag cgcagaagtg gtcctgcaac 1680tttatccgcc
tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
1740agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
cacgctcgtc 1800gtttggtatg gcttcattca gctccggttc ccaacgatca
aggcgagtta catgatcccc 1860catgttgtgc aaaaaagcgg ttagctcctt
cggtcctccg atcgttgtca gaagtaagtt 1920ggccgcagtg ttatcactca
tggttatggc agcactgcat aattctctta ctgtcatgcc 1980atccgtaaga
tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
2040tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
cgccacatag 2100cagaacttta aaagtgctca tcattggaaa acgttcttcg
gggcgaaaac tctcaaggat 2160cttaccgctg ttgagatcca gttcgatgta
acccactcgt gcacccaact gatcttcagc 2220atcttttact ttcaccagcg
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 2280aaagggaata
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
2340ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
gtatttagaa 2400aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
gtgccacctg acgtctaaga 2460aaccattatt atcatgacat taacctataa
aaataggcgt atcacgaggc cctttcgtct 2520cgcgcgtttc ggtgatgacg
gtgaaaacct ctgacacatg cagctcccgg agacggtcac 2580agcttgtctg
taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
2640tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
tgagagtgca 2700ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
aaataccgca tcaggcgcca 2760ttcgccattc aggctgcgca actgttggga
agggcgatcg gtgcgggcct cttcgctatt 2820acgccagctg gcgaaagggg
gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 2880ttcccagtca
cgacgttgta aaacgacggc cagtgaattc gagctcggta cccggggatc
2940ctctagacct gcaggccaac tgcgtttggg gctccagatt aaacgacgcc
gtttcgttcc 3000tttcgcttca cggcttaacg atgtcgtttc tgtctgtgcc
caaaaaataa aggcatttgt 3060tatttgcacc agatatttac taagtgcacc
ctagtttgac aagtaggcga taattacaaa 3120tagatgcggt gcaaataata
aattttgaag gaaataatta caaaagaaca gaacttatat 3180ttactttatt
ttaaaaaact aaaatgaaag aacaaaaaaa gtaaaaaata caaaaaatgt
3240gctttaacca ctttcattat ttgttacaga aagtatgatt ctactcaaat
tgatctgttg 3300tatctggtgc tgccttgtca cactggcgat ttcaatcccc
taaagatatg gtgcaaactg 3360cgaagtgatc aatatctgct cggttaattt
agattaatta ataatattca acgtgatgta 3420ccaaaaaaag acaatttttt
gctccattga caaattaaac ctcatcaagg taatttccaa 3480acctataagc
aaaaaaattt cacattaatt ggcccgcaat cctattagtc ttattatact
3540agagtaggaa aaaaaacaat tacacaactt gtcttattat tctctatgct
aatgaatatt 3600tttccctttt gttagaaatc agtgtttcct aatttattga
gtattaattc cactcaccgc 3660atatatttac cgttgaataa gaaaatttta
cacataattc tttttaagat aaataatttt 3720tttatactag atcttatatg
attacgtgaa gccaagtggg ttatactaat gatatataat 3780gtttgatagt
aatcagttta taaaccaaat gcatggaaat gttacgtgga agcacgtaaa
3840ttaacaagca ttgaagcaaa tgcagccacc gcaccaaaac caccccactt
cacttccacg 3900taccatattc catgcaacta caacacccta aaacttcaat
aaatgccccc accttcactt 3960cacttcaccc atcaatagca agcggccgca
ccatggaggt ggtgaatgaa atagtctcaa 4020ttgggcagga agttttaccc
aaagttgatt atgcccaact ctggagtgat gccagtcact 4080gtgaggtgct
ttacttgtcc atcgcatttg tcatcttgaa gttcactctt ggcccccttg
4140gtccaaaagg tcagtctcgt atgaagtttg ttttcaccaa ttacaacctt
ctcatgtcca 4200tttattcgtt gggatcattc ctctcaatgg catatgccat
gtacaccatc ggtgttatgt 4260ctgacaactg cgagaaggct tttgacaaca
acgtcttcag gatcaccacg cagttgttct 4320atttgagcaa gttcctggag
tatattgact ccttctattt gccactgatg ggcaagcctc 4380tgacctggtt
gcaattcttc catcatttgg gggcaccgat ggatatgtgg ctgttctata
4440attaccgaaa tgaagctgtt tggatttttg tgctgttgaa tggtttcatc
cactggatca 4500tgtacggtta ttattggacc agattgatca agctgaagtt
ccccatgcca aaatccctga 4560ttacatcaat gcagatcatt caattcaatg
ttggtttcta cattgtctgg aagtacagga 4620acattccctg ttatcgccaa
gatgggatga ggatgtttgg ctggttcttc aattactttt 4680atgttggcac
agtcttgtgt ttgttcttga atttctatgt gcaaacgtat atcgtcagga
4740agcacaaggg agccaaaaag attcagtgag c 4771385492DNAArtificial
Sequenceplasmid pKR287 38ggccgcattt cgcaccaaat caatgaaagt
aataatgaaa agtctgaata agaatactta 60ggcttagatg cctttgttac ttgtgtaaaa
taacttgagt catgtacctt tggcggaaac 120agaataaata aaaggtgaaa
ttccaatgct ctatgtataa gttagtaata cttaatgtgt 180tctacggttg
tttcaatatc atcaaactct aattgaaact ttagaaccac aaatctcaat
240cttttcttaa tgaaatgaaa aatcttaatt gtaccatgtt tatgttaaac
accttacaat 300tggttggaga ggaggaccaa ccgatgggac aacattggga
gaaagagatt caatggagat 360ttggatagga gaacaacatt ctttttcact
tcaatacaag atgagtgcaa cactaaggat 420atgtatgaga ctttcagaag
ctacgacaac atagatgagt gaggtggtga ttcctagcaa 480gaaagacatt
agaggaagcc aaaatcgaac aaggaagaca tcaagggcaa gagacaggac
540catccatctc aggaaaagga gctttgggat agtccgagaa gttgtacaag
aaattttttg 600gagggtgagt gatgcattgc tggtgacttt aactcaatca
aaattgagaa agaaagaaaa 660gggagggggc tcacatgtga atagaaggga
aacgggagaa ttttacagtt ttgatctaat 720gggcatccca gctagtggta
acatattcac catgtttaac cttcacgtac gtctagagga 780tccccgggta
ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
840ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
gctggcgtaa 900tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
cgcagcctga atggcgaatg 960gcgcctgatg cggtattttc
tccttacgca tctgtgcggt atttcacacc gcatatggtg 1020cactctcagt
acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
1080acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
gacaagctgt 1140gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
tcatcaccga aacgcgcgag 1200acgaaagggc ctcgtgatac gcctattttt
ataggttaat gtcatgataa taatggtttc 1260ttagacgtca ggtggcactt
ttcggggaaa tgtgcgcgga acccctattt gtttattttt 1320ctaaatacat
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
1380atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
ttcccttttt 1440tgcggcattt tgccttcctg tttttgctca cccagaaacg
ctggtgaaag taaaagatgc 1500tgaagatcag ttgggtgcac gagtgggtta
catcgaactg gatctcaaca gcggtaagat 1560ccttgagagt tttcgccccg
aagaacgttt tccaatgatg agcactttta aagttctgct 1620atgtggcgcg
gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
1680ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
ttacggatgg 1740catgacagta agagaattat gcagtgctgc cataaccatg
agtgataaca ctgcggccaa 1800cttacttctg acaacgatcg gaggaccgaa
ggagctaacc gcttttttgc acaacatggg 1860ggatcatgta actcgccttg
atcgttggga accggagctg aatgaagcca taccaaacga 1920cgagcgtgac
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
1980cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
cggataaagt 2040tgcaggacca cttctgcgct cggcccttcc ggctggctgg
tttattgctg ataaatctgg 2100agccggtgag cgtgggtctc gcggtatcat
tgcagcactg gggccagatg gtaagccctc 2160ccgtatcgta gttatctaca
cgacggggag tcaggcaact atggatgaac gaaatagaca 2220gatcgctgag
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
2280atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
aggtgaagat 2340cctttttgat aatctcatga ccaaaatccc ttaacgtgag
ttttcgttcc actgagcgtc 2400agaccccgta gaaaagatca aaggatcttc
ttgagatcct ttttttctgc gcgtaatctg 2460ctgcttgcaa acaaaaaaac
caccgctacc agcggtggtt tgtttgccgg atcaagagct 2520accaactctt
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
2580tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
ctacatacct 2640cgctctgcta atcctgttac cagtggctgc tgccagtggc
gataagtcgt gtcttaccgg 2700gttggactca agacgatagt taccggataa
ggcgcagcgg tcgggctgaa cggggggttc 2760gtgcacacag cccagcttgg
agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 2820gctatgagaa
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2880cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
ggtatcttta 2940tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
tttttgtgat gctcgtcagg 3000ggggcggagc ctatggaaaa acgccagcaa
cgcggccttt ttacggttcc tggccttttg 3060ctggcctttt gctcacatgt
tctttcctgc gttatcccct gattctgtgg ataaccgtat 3120taccgccttt
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
3180agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
cgcgttggcc 3240gattcattaa tgcagctggc acgacaggtt tcccgactgg
aaagcgggca gtgagcgcaa 3300cgcaattaat gtgagttagc tcactcatta
ggcaccccag gctttacact ttatgcttcc 3360ggctcgtatg ttgtgtggaa
ttgtgagcgg ataacaattt cacacaggaa acagctatga 3420ccatgattac
gccaagcttg catgcctgca ggctagccta agtacgtact caaaatgcca
3480acaaataaaa aaaaagttgc tttaataatg ccaaaacaaa ttaataaaac
acttacaaca 3540ccggattttt tttaattaaa atgtgccatt taggataaat
agttaatatt tttaataatt 3600atttaaaaag ccgtatctac taaaatgatt
tttatttggt tgaaaatatt aatatgttta 3660aatcaacaca atctatcaaa
attaaactaa aaaaaaaata agtgtacgtg gttaacatta 3720gtacagtaat
ataagaggaa aatgagaaat taagaaattg aaagcgagtc taatttttaa
3780attatgaacc tgcatatata aaaggaaaga aagaatccag gaagaaaaga
aatgaaacca 3840tgcatggtcc cctcgtcatc acgagtttct gccatttgca
atagaaacac tgaaacacct 3900ttctctttgt cacttaattg agatgccgaa
gccacctcac accatgaact tcatgaggtg 3960tagcacccaa ggcttccata
gccatgcata ctgaagaatg tctcaagctc agcaccctac 4020ttctgtgacg
tgtccctcat tcaccttcct ctcttcccta taaataacca cgcctcaggt
4080tctccgcttc acaactcaaa cattctctcc attggtcctt aaacactcat
cagtcatcac 4140cgcggccgca tgggaacgga ccaaggaaaa accttcacct
gggaagagct ggcggcccat 4200aacaccaagg acgacctact cttggccatc
cgcggcaggg tgtacgatgt cacaaagttc 4260ttgagccgcc atcctggtgg
agtggacact ctcctgctcg gagctggccg agatgttact 4320ccggtctttg
agatgtatca cgcgtttggg gctgcagatg ccattatgaa gaagtactat
4380gtcggtacac tggtctcgaa tgagctgccc atcttcccgg agccaacggt
gttccacaaa 4440accatcaaga cgagagtcga gggctacttt acggatcgga
acattgatcc caagaataga 4500ccagagatct ggggacgata cgctcttatc
tttggatcct tgatcgcttc ctactacgcg 4560cagctctttg tgcctttcgt
tgtcgaacgc acatggcttc aggtggtgtt tgcaatcatc 4620atgggatttg
cgtgcgcaca agtcggactc aaccctcttc atgatgcgtc tcacttttca
4680gtgacccaca accccactgt ctggaagatt ctgggagcca cgcacgactt
tttcaacgga 4740gcatcgtacc tggtgtggat gtaccaacat atgctcggcc
atcaccccta caccaacatt 4800gctggagcag atcccgacgt gtcgacgtct
gagcccgatg ttcgtcgtat caagcccaac 4860caaaagtggt ttgtcaacca
catcaaccag cacatgtttg ttcctttcct gtacggactg 4920ctggcgttca
aggtgcgcat tcaggacatc aacattttgt actttgtcaa gaccaatgac
4980gctattcgtg tcaatcccat ctcgacatgg cacactgtga tgttctgggg
cggcaaggct 5040ttctttgtct ggtatcgcct gattgttccc ctgcagtatc
tgcccctggg caaggtgctg 5100ctcttgttca cggtcgcgga catggtgtcg
tcttactggc tggcgctgac cttccaggcg 5160aaccacgttg ttgaggaagt
tcagtggccg ttgcctgacg agaacgggat catccaaaag 5220gactgggcag
ctatgcaggt cgagactacg caggattacg cacacgattc gcacctctgg
5280accagcatca ctggcagctt gaactaccag gctgtgcacc atctgttccc
caacgtgtcg 5340cagcaccatt atcccgatat tctggccatc atcaagaaca
cctgcagcga gtacaaggtt 5400ccataccttg tcaaggatac gttttggcaa
gcatttgctt cacatttgga gcacttgcgt 5460gttcttggac tccgtcccaa
ggaagagtag gc 5492391338DNAMortierella alpina 39atgggaacgg
accaaggaaa aaccttcacc tgggaagagc tggcggccca taacaccaag 60gacgacctac
tcttggccat ccgcggcagg gtgtacgatg tcacaaagtt cttgagccgc
120catcctggtg gagtggacac tctcctgctc ggagctggcc gagatgttac
tccggtcttt 180gagatgtatc acgcgtttgg ggctgcagat gccattatga
agaagtacta tgtcggtaca 240ctggtctcga atgagctgcc catcttcccg
gagccaacgg tgttccacaa aaccatcaag 300acgagagtcg agggctactt
tacggatcgg aacattgatc ccaagaatag accagagatc 360tggggacgat
acgctcttat ctttggatcc ttgatcgctt cctactacgc gcagctcttt
420gtgcctttcg ttgtcgaacg cacatggctt caggtggtgt ttgcaatcat
catgggattt 480gcgtgcgcac aagtcggact caaccctctt catgatgcgt
ctcacttttc agtgacccac 540aaccccactg tctggaagat tctgggagcc
acgcacgact ttttcaacgg agcatcgtac 600ctggtgtgga tgtaccaaca
tatgctcggc catcacccct acaccaacat tgctggagca 660gatcccgacg
tgtcgacgtc tgagcccgat gttcgtcgta tcaagcccaa ccaaaagtgg
720tttgtcaacc acatcaacca gcacatgttt gttcctttcc tgtacggact
gctggcgttc 780aaggtgcgca ttcaggacat caacattttg tactttgtca
agaccaatga cgctattcgt 840gtcaatccca tctcgacatg gcacactgtg
atgttctggg gcggcaaggc tttctttgtc 900tggtatcgcc tgattgttcc
cctgcagtat ctgcccctgg gcaaggtgct gctcttgttc 960acggtcgcgg
acatggtgtc gtcttactgg ctggcgctga ccttccaggc gaaccacgtt
1020gttgaggaag ttcagtggcc gttgcctgac gagaacggga tcatccaaaa
ggactgggca 1080gctatgcagg tcgagactac gcaggattac gcacacgatt
cgcacctctg gaccagcatc 1140actggcagct tgaactacca ggctgtgcac
catctgttcc ccaacgtgtc gcagcaccat 1200tatcccgata ttctggccat
catcaagaac acctgcagcg agtacaaggt tccatacctt 1260gtcaaggata
cgttttggca agcatttgct tcacatttgg agcacttgcg tgttcttgga
1320ctccgtccca aggaagag 1338402577DNAArtificial Sequenceplasmid
pKR277 40agcttggatc tcctgcagga tctggccggc cggatctcgt acggatccgt
cgacggcgcg 60cccgatcatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac
ccgtttagag 120gccccaaggg gttatgctag ttattgctca gcggtggcag
cagccaactc agcttccttt 180cgggctttgt tagcagccgg atcgatccaa
gctgtacctc actattcctt tgccctcgga 240cgagtgctgg ggcgtcggtt
tccactatcg gcgagtactt ctacacagcc atcggtccag 300acggccgcgc
ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
360attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
aaccaagctc 420tgatagagtt ggtcaagacc aatgcggagc atatacgccc
ggagccgcgg cgatcctgca 480agctccggat gcctccgctc gaagtagcgc
gtctgctgct ccatacaagc caaccacggc 540ctccagaaga agatgttggc
gacctcgtat tgggaatccc cgaacatcgc ctcgctccag 600tcaatgaccg
ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
660tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
atcgagagcc 720tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
gccagtgata cacatgggga 780tcagcaatcg cgcatatgaa atcacgccat
gtagtgtatt gaccgattcc ttgcggtccg 840aatgggccga acccgctcgt
ctggctaaga tcggccgcag cgatcgcatc catagcctcc 900gcgaccggct
gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
960tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
gtcaagcact 1020tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
cataacgatc tttgtagaaa 1080ccatcggcgc agctatttac ccgcaggaca
tatccacgcc ctcctacatc gaagctgaaa 1140gcacgagatt cttcgccctc
cgagagctgc atcaggtcgg agacgctgtc gaacttttcg 1200atcagaaact
tctcgacaga cgtcgcggtg agttcaggct tttccatggg tatatctcct
1260tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
tatagtgagt 1320cgtattaatt tcgcgggatc gagatctgat caacctgcat
taatgaatcg gccaacgcgc 1380ggggagaggc ggtttgcgta ttgggcgctc
ttccgcttcc tcgctcactg actcgctgcg 1440ctcggtcgtt cggctgcggc
gagcggtatc agctcactca aaggcggtaa tacggttatc 1500cacagaatca
ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1560gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
ctgacgagca 1620tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
acaggactat aaagatacca 1680ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt ccgaccctgc cgcttaccgg 1740atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt tctcaatgct cacgctgtag 1800gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
1860tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
cggtaagaca 1920cgacttatcg ccactggcag cagccactgg taacaggatt
agcagagcga ggtatgtagg 1980cggtgctaca gagttcttga agtggtggcc
taactacggc tacactagaa ggacagtatt 2040tggtatctgc gctctgctga
agccagttac cttcggaaaa agagttggta gctcttgatc 2100cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2160cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
acgctcagtg 2220gaacgaaaac tcacgttaag ggattttggt catgacatta
acctataaaa ataggcgtat 2280cacgaggccc tttcgtctcg cgcgtttcgg
tgatgacggt gaaaacctct gacacatgca 2340gctcccggag acggtcacag
cttgtctgta agcggatgcc gggagcagac aagcccgtca 2400gggcgcgtca
gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
2460gattgtactg agagtgcacc atatggacat attgtcgtta gaacgcggct
acaattaata 2520cataacctta tgtatcatac acatacgatt taggtgacac
tatagaacgg cgcgcca 2577415364DNAArtificial Sequenceplasmid pKR952
41ggctagccta agtacgtact caaaatgcca acaaataaaa aaaaagttgc tttaataatg
60ccaaaacaaa ttaataaaac acttacaaca ccggattttt tttaattaaa atgtgccatt
120taggataaat agttaatatt tttaataatt atttaaaaag ccgtatctac
taaaatgatt 180tttatttggt tgaaaatatt aatatgttta aatcaacaca
atctatcaaa attaaactaa 240aaaaaaaata agtgtacgtg gttaacatta
gtacagtaat ataagaggaa aatgagaaat 300taagaaattg aaagcgagtc
taatttttaa attatgaacc tgcatatata aaaggaaaga 360aagaatccag
gaagaaaaga aatgaaacca tgcatggtcc cctcgtcatc acgagtttct
420gccatttgca atagaaacac tgaaacacct ttctctttgt cacttaattg
agatgccgaa 480gccacctcac accatgaact tcatgaggtg tagcacccaa
ggcttccata gccatgcata 540ctgaagaatg tctcaagctc agcaccctac
ttctgtgacg tgtccctcat tcaccttcct 600ctcttcccta taaataacca
cgcctcaggt tctccgcttc acaactcaaa cattctctcc 660attggtcctt
aaacactcat cagtcatcac cgcggccgca tgggaacgga ccaaggaaaa
720accttcacct gggaagagct ggcggcccat aacaccaagg acgacctact
cttggccatc 780cgcggcaggg tgtacgatgt cacaaagttc ttgagccgcc
atcctggtgg agtggacact 840ctcctgctcg gagctggccg agatgttact
ccggtctttg agatgtatca cgcgtttggg 900gctgcagatg ccattatgaa
gaagtactat gtcggtacac tggtctcgaa tgagctgccc 960atcttcccgg
agccaacggt gttccacaaa accatcaaga cgagagtcga gggctacttt
1020acggatcgga acattgatcc caagaataga ccagagatct ggggacgata
cgctcttatc 1080tttggatcct tgatcgcttc ctactacgcg cagctctttg
tgcctttcgt tgtcgaacgc 1140acatggcttc aggtggtgtt tgcaatcatc
atgggatttg cgtgcgcaca agtcggactc 1200aaccctcttc atgatgcgtc
tcacttttca gtgacccaca accccactgt ctggaagatt 1260ctgggagcca
cgcacgactt tttcaacgga gcatcgtacc tggtgtggat gtaccaacat
1320atgctcggcc atcaccccta caccaacatt gctggagcag atcccgacgt
gtcgacgtct 1380gagcccgatg ttcgtcgtat caagcccaac caaaagtggt
ttgtcaacca catcaaccag 1440cacatgtttg ttcctttcct gtacggactg
ctggcgttca aggtgcgcat tcaggacatc 1500aacattttgt actttgtcaa
gaccaatgac gctattcgtg tcaatcccat ctcgacatgg 1560cacactgtga
tgttctgggg cggcaaggct ttctttgtct ggtatcgcct gattgttccc
1620ctgcagtatc tgcccctggg caaggtgctg ctcttgttca cggtcgcgga
catggtgtcg 1680tcttactggc tggcgctgac cttccaggcg aaccacgttg
ttgaggaagt tcagtggccg 1740ttgcctgacg agaacgggat catccaaaag
gactgggcag ctatgcaggt cgagactacg 1800caggattacg cacacgattc
gcacctctgg accagcatca ctggcagctt gaactaccag 1860gctgtgcacc
atctgttccc caacgtgtcg cagcaccatt atcccgatat tctggccatc
1920atcaagaaca cctgcagcga gtacaaggtt ccataccttg tcaaggatac
gttttggcaa 1980gcatttgctt cacatttgga gcacttgcgt gttcttggac
tccgtcccaa ggaagagtag 2040gcggccgcat ttcgcaccaa atcaatgaaa
gtaataatga aaagtctgaa taagaatact 2100taggcttaga tgcctttgtt
acttgtgtaa aataacttga gtcatgtacc tttggcggaa 2160acagaataaa
taaaaggtga aattccaatg ctctatgtat aagttagtaa tacttaatgt
2220gttctacggt tgtttcaata tcatcaaact ctaattgaaa ctttagaacc
acaaatctca 2280atcttttctt aatgaaatga aaaatcttaa ttgtaccatg
tttatgttaa acaccttaca 2340attggttgga gaggaggacc aaccgatggg
acaacattgg gagaaagaga ttcaatggag 2400atttggatag gagaacaaca
ttctttttca cttcaataca agatgagtgc aacactaagg 2460atatgtatga
gactttcaga agctacgaca acatagatga gtgaggtggt gattcctagc
2520aagaaagaca ttagaggaag ccaaaatcga acaaggaaga catcaagggc
aagagacagg 2580accatccatc tcaggaaaag gagctttggg atagtccgag
aagttgtaca agaaattttt 2640tggagggtga gtgatgcatt gctggtgact
ttaactcaat caaaattgag aaagaaagaa 2700aagggagggg gctcacatgt
gaatagaagg gaaacgggag aattttacag ttttgatcta 2760atgggcatcc
cagctagtgg taacatattc accatgttta accttcacgt acggatccgt
2820cgacggcgcg cccgatcatc cggatatagt tcctcctttc agcaaaaaac
ccctcaagac 2880ccgtttagag gccccaaggg gttatgctag ttattgctca
gcggtggcag cagccaactc 2940agcttccttt cgggctttgt tagcagccgg
atcgatccaa gctgtacctc actattcctt 3000tgccctcgga cgagtgctgg
ggcgtcggtt tccactatcg gcgagtactt ctacacagcc 3060atcggtccag
acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
3120ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
aattgccgtc 3180aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
atatacgccc ggagccgcgg 3240cgatcctgca agctccggat gcctccgctc
gaagtagcgc gtctgctgct ccatacaagc 3300caaccacggc ctccagaaga
agatgttggc gacctcgtat tgggaatccc cgaacatcgc 3360ctcgctccag
tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
3420gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
gcatcagctc 3480atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
atcacagttt gccagtgata 3540cacatgggga tcagcaatcg cgcatatgaa
atcacgccat gtagtgtatt gaccgattcc 3600ttgcggtccg aatgggccga
acccgctcgt ctggctaaga tcggccgcag cgatcgcatc 3660catagcctcc
gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
3720cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
attccccaat 3780gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
tgccgataaa cataacgatc 3840tttgtagaaa ccatcggcgc agctatttac
ccgcaggaca tatccacgcc ctcctacatc 3900gaagctgaaa gcacgagatt
cttcgccctc cgagagctgc atcaggtcgg agacgctgtc 3960gaacttttcg
atcagaaact tctcgacaga cgtcgcggtg agttcaggct tttccatggg
4020tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt
gtggtctccc 4080tatagtgagt cgtattaatt tcgcgggatc gagatctgat
caacctgcat taatgaatcg 4140gccaacgcgc ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc tcgctcactg 4200actcgctgcg ctcggtcgtt
cggctgcggc gagcggtatc agctcactca aaggcggtaa 4260tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4320aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
ctccgccccc 4380ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg acaggactat 4440aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt ccgaccctgc 4500cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 4560cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
4620aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
gagtccaacc 4680cggtaagaca cgacttatcg ccactggcag cagccactgg
taacaggatt agcagagcga 4740ggtatgtagg cggtgctaca gagttcttga
agtggtggcc taactacggc tacactagaa 4800ggacagtatt tggtatctgc
gctctgctga agccagttac cttcggaaaa agagttggta 4860gctcttgatc
cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
4920agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
acggggtctg 4980acgctcagtg gaacgaaaac tcacgttaag ggattttggt
catgacatta acctataaaa 5040ataggcgtat cacgaggccc tttcgtctcg
cgcgtttcgg tgatgacggt gaaaacctct 5100gacacatgca gctcccggag
acggtcacag cttgtctgta agcggatgcc gggagcagac 5160aagcccgtca
gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
5220catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
gaacgcggct 5280acaattaata cataacctta tgtatcatac acatacgatt
taggtgacac tatagaacgg 5340cgcgccaagc ttggatctcc tgca
5364425252DNAArtificial Sequenceplasmid pKR457 42gtacgtgggc
ggatcccccg ggctgcagga attcactggc cgtcgtttta caacgtcgtg 60actgggaaaa
ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
120gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
cgcagcctga 180atggcgaatg gcgcctgatg cggtattttc tccttacgca
tctgtgcggt atttcacacc 240gcatatggtg cactctcagt acaatctgct
ctgatgccgc atagttaagc cagccccgac 300acccgccaac acccgctgac
gcgccctgac gggcttgtct gctcccggca tccgcttaca 360gacaagctgt
gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
420aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
gtcatgataa 480taatggtttc ttagacgtca ggtggcactt ttcggggaaa
tgtgcgcgga acccctattt 540gtttattttt ctaaatacat tcaaatatgt
atccgctcat gagacaataa ccctgataaa 600tgcttcaata atattgaaaa
aggaagagta tgagtattca acatttccgt gtcgccctta 660ttcccttttt
tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
720taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
gatctcaaca 780gcggtaagat ccttgagagt tttcgccccg aagaacgttt
tccaatgatg agcactttta 840aagttctgct atgtggcgcg gtattatccc
gtattgacgc cgggcaagag caactcggtc 900gccgcataca ctattctcag
aatgacttgg ttgagtactc accagtcaca gaaaagcatc 960ttacggatgg
catgacagta agagaattat gcagtgctgc
cataaccatg agtgataaca 1020ctgcggccaa cttacttctg acaacgatcg
gaggaccgaa ggagctaacc gcttttttgc 1080acaacatggg ggatcatgta
actcgccttg atcgttggga accggagctg aatgaagcca 1140taccaaacga
cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
1200tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
tggatggagg 1260cggataaagt tgcaggacca cttctgcgct cggcccttcc
ggctggctgg tttattgctg 1320ataaatctgg agccggtgag cgtgggtctc
gcggtatcat tgcagcactg gggccagatg 1380gtaagccctc ccgtatcgta
gttatctaca cgacggggag tcaggcaact atggatgaac 1440gaaatagaca
gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
1500aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
aaaaggatct 1560aggtgaagat cctttttgat aatctcatga ccaaaatccc
ttaacgtgag ttttcgttcc 1620actgagcgtc agaccccgta gaaaagatca
aaggatcttc ttgagatcct ttttttctgc 1680gcgtaatctg ctgcttgcaa
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 1740atcaagagct
accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
1800atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
gtagcaccgc 1860ctacatacct cgctctgcta atcctgttac cagtggctgc
tgccagtggc gataagtcgt 1920gtcttaccgg gttggactca agacgatagt
taccggataa ggcgcagcgg tcgggctgaa 1980cggggggttc gtgcacacag
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 2040tacagcgtga
gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
2100cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
ggaaacgcct 2160ggtatcttta tagtcctgtc gggtttcgcc acctctgact
tgagcgtcga tttttgtgat 2220gctcgtcagg ggggcggagc ctatggaaaa
acgccagcaa cgcggccttt ttacggttcc 2280tggccttttg ctggcctttt
gctcacatgt tctttcctgc gttatcccct gattctgtgg 2340ataaccgtat
taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
2400gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
cctctccccg 2460cgcgttggcc gattcattaa tgcagctggc acgacaggtt
tcccgactgg aaagcgggca 2520gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact 2580ttatgcttcc ggctcgtatg
ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 2640acagctatga
ccatgattac gccaagcttg catgcctgca ggtcgactcg acgtacgtcc
2700tcgaagagaa gggttaataa cacatttttt aacattttta acacaaattt
tagttattta 2760aaaatttatt aaaaaattta aaataagaag aggaactctt
taaataaatc taacttacaa 2820aatttatgat ttttaataag ttttcaccaa
taaaaaatgt cataaaaata tgttaaaaag 2880tatattatca atattctctt
tatgataaat aaaaagaaaa aaaaaataaa agttaagtga 2940aaatgagatt
gaagtgactt taggtgtgta taaatatatc aaccccgcca acaatttatt
3000taatccaaat atattgaagt atattattcc atagccttta tttatttata
tatttattat 3060ataaaagctt tatttgttct aggttgttca tgaaatattt
ttttggtttt atctccgttg 3120taagaaaatc atgtgctttg tgtcgccact
cactattgca gctttttcat gcattggtca 3180gattgacggt tgattgtatt
tttgtttttt atggttttgt gttatgactt aagtcttcat 3240ctctttatct
cttcatcagg tttgatggtt acctaatatg gtccatgggt acatgcatgg
3300ttaaattagg tggccaactt tgttgtgaac gatagaattt tttttatatt
aagtaaacta 3360tttttatatt atgaaataat aataaaaaaa atattttatc
attattaaca aaatcatatt 3420agttaatttg ttaactctat aataaaagaa
atactgtaac attcacatta catggtaaca 3480tctttccacc ctttcatttg
ttttttgttt gatgactttt tttcttgttt aaatttattt 3540cccttctttt
aaatttggaa tacattatca tcatatataa actaaaatac taaaaacagg
3600attacacaaa tgataaataa taacacaaat atttataaat ctagctgcaa
tatatttaaa 3660ctagctatat cgatattgta aaataaaact agctgcattg
atactgataa aaaaatatca 3720tgtgctttct ggactgatga tgcagtatac
ttttgacatt gcctttattt tatttttcag 3780aaaagctttc ttagttctgg
gttcttcatt atttgtttcc catctccatt gtgaattgaa 3840tcatttgctt
cgtgtcacaa atacaattta gntaggtaca tgcattggtc agattcacgg
3900tttattatgt catgacttaa gttcatggta gtacattacc tgccacgcat
gcattatatt 3960ggttagattt gataggcaaa tttggttgtc aacaatataa
atataaataa tgtttttata 4020ttacgaaata acagtgatca aaacaaacag
ttttatcttt attaacaaga ttttgttttt 4080gtttgatgac gttttttaat
gtttacgctt tcccccttct tttgaattta gaacacttta 4140tcatcataaa
atcaaatact aaaaaaatta catatttcat aaataataac acaaatattt
4200ttaaaaaatc tgaaataata atgaacaata ttacatatta tcacgaaaat
tcattaataa 4260aaatattata taaataaaat gtaatagtag ttatatgtag
gaaaaaagta ctgcacgcat 4320aatatataca aaaagattaa aatgaactat
tataaataat aacactaaat taatggtgaa 4380tcatatcaaa ataatgaaaa
agtaaataaa atttgtaatt aacttctata tgtattacac 4440acacaaataa
taaataatag taaaaaaaat tatgataaat atttaccatc tcataagata
4500tttaaaataa tgataaaaat atagattatt ttttatgcaa ctagctagcc
aaaaagagaa 4560cacgggtata tataaaaaga gtacctttaa attctactgt
acttccttta ttcctgacgt 4620ttttatatca agtggacata cgtgaagatt
ttaattatca gtctaaatat ttcattagca 4680cttaatactt ttctgtttta
ttcctatcct ataagtagtc ccgattctcc caacattgct 4740tattcacaca
actaactaag aaagtcttcc atagcccccc aagcggccgc gacacaagtg
4800tgagagtact aaataaatgc tttggttgta cgaaatcatt acactaaata
aaataatcaa 4860agcttatata tgccttccgc taaggccgaa tgcaaagaaa
ttggttcttt ctcgttatct 4920tttgccactt ttactagtac gtattaatta
ctacttaatc atctttgttt acggctcatt 4980atatccggtc tagaggatcc
aaggccgcga agttaaaagc aatgttgtca cttgtcgtac 5040taacacatga
tgtgatagtt tatgctagct agctataaca taagctgtct ctgagtgtgt
5100tgtatattaa taaagatcat cactggtgaa tggtgatcgt gtacgtaccc
tacttagtag 5160gcaatggaag cacttagagt gtgctttgtg catggccttg
cctctgtttt gagacttttg 5220taatgttttc gagtttaaat ctttgccttt gc
5252432635DNAArtificial Sequencemodified Kti/NotI/Kti3'Salb3'
cassette 43ggtaccgggc cccccctcga ggtcgcccgg gggatccgcc ctaagcttcg
tacgtcctcg 60aagagaaggg ttaataacac attttttaac atttttaaca caaattttag
ttatttaaaa 120atttattaaa aaatttaaaa taagaagagg aactctttaa
ataaatctaa cttacaaaat 180ttatgatttt taataagttt tcaccaataa
aaaatgtcat aaaaatatgt taaaaagtat 240attatcaata ttctctttat
gataaataaa aagaaaaaaa aaataaaagt taagtgaaaa 300tgagattgaa
gtgactttag gtgtgtataa atatatcaac cccgccaaca atttatttaa
360tccaaatata ttgaagtata ttattccata gcctttattt atttatatat
ttattatata 420aaagctttat ttgttctagg ttgttcatga aatatttttt
tggttttatc tccgttgtaa 480gaaaatcatg tgctttgtgt cgccactcac
tattgcagct ttttcatgca ttggtcagat 540tgacggttga ttgtattttt
gttttttatg gttttgtgtt atgacttaag tcttcatctc 600tttatctctt
catcaggttt gatggttacc taatatggtc catgggtaca tgcatggtta
660aattaggtgg ccaactttgt tgtgaacgat agaatttttt ttatattaag
taaactattt 720ttatattatg aaataataat aaaaaaaata ttttatcatt
attaacaaaa tcatattagt 780taatttgtta actctataat aaaagaaata
ctgtaacatt cacattacat ggtaacatct 840ttccaccctt tcatttgttt
tttgtttgat gacttttttt cttgtttaaa tttatttccc 900ttcttttaaa
tttggaatac attatcatca tatataaact aaaatactaa aaacaggatt
960acacaaatga taaataataa cacaaatatt tataaatcta gctgcaatat
atttaaacta 1020gctatatcga tattgtaaaa taaaactagc tgcattgata
ctgataaaaa aatatcatgt 1080gctttctgga ctgatgatgc agtatacttt
tgacattgcc tttattttat ttttcagaaa 1140agctttctta gttctgggtt
cttcattatt tgtttcccat ctccattgtg aattgaatca 1200tttgcttcgt
gtcacaaata caatttagnt aggtacatgc attggtcaga ttcacggttt
1260attatgtcat gacttaagtt catggtagta cattacctgc cacgcatgca
ttatattggt 1320tagatttgat aggcaaattt ggttgtcaac aatataaata
taaataatgt ttttatatta 1380cgaaataaca gtgatcaaaa caaacagttt
tatctttatt aacaagattt tgtttttgtt 1440tgatgacgtt ttttaatgtt
tacgctttcc cccttctttt gaatttagaa cactttatca 1500tcataaaatc
aaatactaaa aaaattacat atttcataaa taataacaca aatattttta
1560aaaaatctga aataataatg aacaatatta catattatca cgaaaattca
ttaataaaaa 1620tattatataa ataaaatgta atagtagtta tatgtaggaa
aaaagtactg cacgcataat 1680atatacaaaa agattaaaat gaactattat
aaataataac actaaattaa tggtgaatca 1740tatcaaaata atgaaaaagt
aaataaaatt tgtaattaac ttctatatgt attacacaca 1800caaataataa
ataatagtaa aaaaaattat gataaatatt taccatctca taagatattt
1860aaaataatga taaaaatata gattattttt tatgcaacta gctagccaaa
aagagaacac 1920gggtatatat aaaaagagta cctttaaatt ctactgtact
tcctttattc ctgacgtttt 1980tatatcaagt ggacatacgt gaagatttta
attatcagtc taaatatttc attagcactt 2040aatacttttc tgttttattc
ctatcctata agtagtcccg attctcccaa cattgcttat 2100tcacacaact
aactaagaaa gtcttccata gccccccaag cggccgcgac acaagtgtga
2160gagtactaaa taaatgcttt ggttgtacga aatcattaca ctaaataaaa
taatcaaagc 2220ttatatatgc cttccgctaa ggccgaatgc aaagaaattg
gttctttctc gttatctttt 2280gccactttta ctagtacgta ttaattacta
cttaatcatc tttgtttacg gctcattata 2340tccggtctag aggatccaag
gccgcgaagt taaaagcaat gttgtcactt gtcgtactaa 2400cacatgatgt
gatagtttat gctagctagc tataacataa gctgtctctg agtgtgttgt
2460atattaataa agatcatcac tggtgaatgg tgatcgtgta cgtaccctac
ttagtaggca 2520atggaagcac ttagagtgtg ctttgtgcat ggccttgcct
ctgttttgag acttttgtaa 2580tgttttcgag tttaaatctt tgcctttgcg
tacgtctaga ggatccccgg gtacc 2635449276DNAArtificial Sequenceplasmid
pKR970 44gtacggatcc gtcgacggcg cgcccgatca tccggatata gttcctcctt
tcagcaaaaa 60acccctcaag acccgtttag aggccccaag gggttatgct agttattgct
cagcggtggc 120agcagccaac tcagcttcct ttcgggcttt gttagcagcc
ggatcgatcc aagctgtacc 180tcactattcc tttgccctcg gacgagtgct
ggggcgtcgg tttccactat cggcgagtac 240ttctacacag ccatcggtcc
agacggccgc gcttctgcgg gcgatttgtg tacgcccgac 300agtcccggct
ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
360gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
gcatatacgc 420ccggagccgc ggcgatcctg caagctccgg atgcctccgc
tcgaagtagc gcgtctgctg 480ctccatacaa gccaaccacg gcctccagaa
gaagatgttg gcgacctcgt attgggaatc 540cccgaacatc gcctcgctcc
agtcaatgac cgctgttatg cggccattgt ccgtcaggac 600attgttggag
ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
660aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
ccatcacagt 720ttgccagtga tacacatggg gatcagcaat cgcgcatatg
aaatcacgcc atgtagtgta 780ttgaccgatt ccttgcggtc cgaatgggcc
gaacccgctc gtctggctaa gatcggccgc 840agcgatcgca tccatagcct
ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg 900caggtcttgc
aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
960gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
agtgccgata 1020aacataacga tctttgtaga aaccatcggc gcagctattt
acccgcagga catatccacg 1080ccctcctaca tcgaagctga aagcacgaga
ttcttcgccc tccgagagct gcatcaggtc 1140ggagacgctg tcgaactttt
cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg 1200cttttccatg
ggtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg
1260ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctg
atcaacctgc 1320attaatgaat cggccaacgc gcggggagag gcggtttgcg
tattgggcgc tcttccgctt 1380cctcgctcac tgactcgctg cgctcggtcg
ttcggctgcg gcgagcggta tcagctcact 1440caaaggcggt aatacggtta
tccacagaat caggggataa cgcaggaaag aacatgtgag 1500caaaaggcca
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
1560ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
tggcgaaacc 1620cgacaggact ataaagatac caggcgtttc cccctggaag
ctccctcgtg cgctctcctg 1680ttccgaccct gccgcttacc ggatacctgt
ccgcctttct cccttcggga agcgtggcgc 1740tttctcaatg ctcacgctgt
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 1800gctgtgtgca
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
1860ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
ggtaacagga 1920ttagcagagc gaggtatgta ggcggtgcta cagagttctt
gaagtggtgg cctaactacg 1980gctacactag aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa 2040aaagagttgg tagctcttga
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 2100tttgcaagca
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
2160ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
gtcatgacat 2220taacctataa aaataggcgt atcacgaggc cctttcgtct
cgcgcgtttc ggtgatgacg 2280gtgaaaacct ctgacacatg cagctcccgg
agacggtcac agcttgtctg taagcggatg 2340ccgggagcag acaagcccgt
cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 2400ttaactatgc
ggcatcagag cagattgtac tgagagtgca ccatatggac atattgtcgt
2460tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
tttaggtgac 2520actatagaac ggcgcgccaa gcttggatct cctgcaggct
agcctaagta cgtactcaaa 2580atgccaacaa ataaaaaaaa agttgcttta
ataatgccaa aacaaattaa taaaacactt 2640acaacaccgg atttttttta
attaaaatgt gccatttagg ataaatagtt aatattttta 2700ataattattt
aaaaagccgt atctactaaa atgattttta tttggttgaa aatattaata
2760tgtttaaatc aacacaatct atcaaaatta aactaaaaaa aaaataagtg
tacgtggtta 2820acattagtac agtaatataa gaggaaaatg agaaattaag
aaattgaaag cgagtctaat 2880ttttaaatta tgaacctgca tatataaaag
gaaagaaaga atccaggaag aaaagaaatg 2940aaaccatgca tggtcccctc
gtcatcacga gtttctgcca tttgcaatag aaacactgaa 3000acacctttct
ctttgtcact taattgagat gccgaagcca cctcacacca tgaacttcat
3060gaggtgtagc acccaaggct tccatagcca tgcatactga agaatgtctc
aagctcagca 3120ccctacttct gtgacgtgtc cctcattcac cttcctctct
tccctataaa taaccacgcc 3180tcaggttctc cgcttcacaa ctcaaacatt
ctctccattg gtccttaaac actcatcagt 3240catcaccgcg gccgcatggg
aacggaccaa ggaaaaacct tcacctggga agagctggcg 3300gcccataaca
ccaaggacga cctactcttg gccatccgcg gcagggtgta cgatgtcaca
3360aagttcttga gccgccatcc tggtggagtg gacactctcc tgctcggagc
tggccgagat 3420gttactccgg tctttgagat gtatcacgcg tttggggctg
cagatgccat tatgaagaag 3480tactatgtcg gtacactggt ctcgaatgag
ctgcccatct tcccggagcc aacggtgttc 3540cacaaaacca tcaagacgag
agtcgagggc tactttacgg atcggaacat tgatcccaag 3600aatagaccag
agatctgggg acgatacgct cttatctttg gatccttgat cgcttcctac
3660tacgcgcagc tctttgtgcc tttcgttgtc gaacgcacat ggcttcaggt
ggtgtttgca 3720atcatcatgg gatttgcgtg cgcacaagtc ggactcaacc
ctcttcatga tgcgtctcac 3780ttttcagtga cccacaaccc cactgtctgg
aagattctgg gagccacgca cgactttttc 3840aacggagcat cgtacctggt
gtggatgtac caacatatgc tcggccatca cccctacacc 3900aacattgctg
gagcagatcc cgacgtgtcg acgtctgagc ccgatgttcg tcgtatcaag
3960cccaaccaaa agtggtttgt caaccacatc aaccagcaca tgtttgttcc
tttcctgtac 4020ggactgctgg cgttcaaggt gcgcattcag gacatcaaca
ttttgtactt tgtcaagacc 4080aatgacgcta ttcgtgtcaa tcccatctcg
acatggcaca ctgtgatgtt ctggggcggc 4140aaggctttct ttgtctggta
tcgcctgatt gttcccctgc agtatctgcc cctgggcaag 4200gtgctgctct
tgttcacggt cgcggacatg gtgtcgtctt actggctggc gctgaccttc
4260caggcgaacc acgttgttga ggaagttcag tggccgttgc ctgacgagaa
cgggatcatc 4320caaaaggact gggcagctat gcaggtcgag actacgcagg
attacgcaca cgattcgcac 4380ctctggacca gcatcactgg cagcttgaac
taccaggctg tgcaccatct gttccccaac 4440gtgtcgcagc accattatcc
cgatattctg gccatcatca agaacacctg cagcgagtac 4500aaggttccat
accttgtcaa ggatacgttt tggcaagcat ttgcttcaca tttggagcac
4560ttgcgtgttc ttggactccg tcccaaggaa gagtaggcgg ccgcatttcg
caccaaatca 4620atgaaagtaa taatgaaaag tctgaataag aatacttagg
cttagatgcc tttgttactt 4680gtgtaaaata acttgagtca tgtacctttg
gcggaaacag aataaataaa aggtgaaatt 4740ccaatgctct atgtataagt
tagtaatact taatgtgttc tacggttgtt tcaatatcat 4800caaactctaa
ttgaaacttt agaaccacaa atctcaatct tttcttaatg aaatgaaaaa
4860tcttaattgt accatgttta tgttaaacac cttacaattg gttggagagg
aggaccaacc 4920gatgggacaa cattgggaga aagagattca atggagattt
ggataggaga acaacattct 4980ttttcacttc aatacaagat gagtgcaaca
ctaaggatat gtatgagact ttcagaagct 5040acgacaacat agatgagtga
ggtggtgatt cctagcaaga aagacattag aggaagccaa 5100aatcgaacaa
ggaagacatc aagggcaaga gacaggacca tccatctcag gaaaaggagc
5160tttgggatag tccgagaagt tgtacaagaa attttttgga gggtgagtga
tgcattgctg 5220gtgactttaa ctcaatcaaa attgagaaag aaagaaaagg
gagggggctc acatgtgaat 5280agaagggaaa cgggagaatt ttacagtttt
gatctaatgg gcatcccagc tagtggtaac 5340atattcacca tgtttaacct
tcacgtaccg ggccccccct cgaggtcgcc cgggggatcc 5400gccctaagct
tcgtacgtcc tcgaagagaa gggttaataa cacatttttt aacattttta
5460acacaaattt tagttattta aaaatttatt aaaaaattta aaataagaag
aggaactctt 5520taaataaatc taacttacaa aatttatgat ttttaataag
ttttcaccaa taaaaaatgt 5580cataaaaata tgttaaaaag tatattatca
atattctctt tatgataaat aaaaagaaaa 5640aaaaaataaa agttaagtga
aaatgagatt gaagtgactt taggtgtgta taaatatatc 5700aaccccgcca
acaatttatt taatccaaat atattgaagt atattattcc atagccttta
5760tttatttata tatttattat ataaaagctt tatttgttct aggttgttca
tgaaatattt 5820ttttggtttt atctccgttg taagaaaatc atgtgctttg
tgtcgccact cactattgca 5880gctttttcat gcattggtca gattgacggt
tgattgtatt tttgtttttt atggttttgt 5940gttatgactt aagtcttcat
ctctttatct cttcatcagg tttgatggtt acctaatatg 6000gtccatgggt
acatgcatgg ttaaattagg tggccaactt tgttgtgaac gatagaattt
6060tttttatatt aagtaaacta tttttatatt atgaaataat aataaaaaaa
atattttatc 6120attattaaca aaatcatatt agttaatttg ttaactctat
aataaaagaa atactgtaac 6180attcacatta catggtaaca tctttccacc
ctttcatttg ttttttgttt gatgactttt 6240tttcttgttt aaatttattt
cccttctttt aaatttggaa tacattatca tcatatataa 6300actaaaatac
taaaaacagg attacacaaa tgataaataa taacacaaat atttataaat
6360ctagctgcaa tatatttaaa ctagctatat cgatattgta aaataaaact
agctgcattg 6420atactgataa aaaaatatca tgtgctttct ggactgatga
tgcagtatac ttttgacatt 6480gcctttattt tatttttcag aaaagctttc
ttagttctgg gttcttcatt atttgtttcc 6540catctccatt gtgaattgaa
tcatttgctt cgtgtcacaa atacaattta gntaggtaca 6600tgcattggtc
agattcacgg tttattatgt catgacttaa gttcatggta gtacattacc
6660tgccacgcat gcattatatt ggttagattt gataggcaaa tttggttgtc
aacaatataa 6720atataaataa tgtttttata ttacgaaata acagtgatca
aaacaaacag ttttatcttt 6780attaacaaga ttttgttttt gtttgatgac
gttttttaat gtttacgctt tcccccttct 6840tttgaattta gaacacttta
tcatcataaa atcaaatact aaaaaaatta catatttcat 6900aaataataac
acaaatattt ttaaaaaatc tgaaataata atgaacaata ttacatatta
6960tcacgaaaat tcattaataa aaatattata taaataaaat gtaatagtag
ttatatgtag 7020gaaaaaagta ctgcacgcat aatatataca aaaagattaa
aatgaactat tataaataat 7080aacactaaat taatggtgaa tcatatcaaa
ataatgaaaa agtaaataaa atttgtaatt 7140aacttctata tgtattacac
acacaaataa taaataatag taaaaaaaat tatgataaat 7200atttaccatc
tcataagata tttaaaataa tgataaaaat atagattatt ttttatgcaa
7260ctagctagcc aaaaagagaa cacgggtata tataaaaaga gtacctttaa
attctactgt 7320acttccttta ttcctgacgt ttttatatca agtggacata
cgtgaagatt ttaattatca 7380gtctaaatat ttcattagca cttaatactt
ttctgtttta ttcctatcct ataagtagtc 7440ccgattctcc caacattgct
tattcacaca actaactaag aaagtcttcc atagcccccc 7500aagcggccgc
accatgggca agggtggaga cggcggcgcg caggcggtga gcgggaccga
7560cgcgtctctc gctgaggtga gctccgtcga tagcaagagc gtgcacgtcg
tgctctacgg 7620caagcgcgtg gatgtcacaa agttccagaa ggcacacccg
ggcgggagca aggtgttccg 7680catcttccag gagcgcgacg cgacggagca
gttcgagtct taccactcgc ccaaggccat 7740caagatgatg gagggcatgc
tcaagaagtc ggaggatgcg cccgcttccg tgcccctgcc 7800ctcgcggtcc
accatgggca cggagttcaa ggagatgatt gagcgccaca agagggctgg
7860tctctacgac ccttgcccgt tggacgagct gttcaagctc accatcgtcc
ttgcgcccat 7920cttcgtgggc gcctatctcg tgcggagcgg cgtctcgccc
ctcgcgggcg cgctctccat 7980gggctttggc ttctacctcg acggctggct
tgctcacgac
tacctgcatc acgcagtctt 8040caagggctcg gtcaacacgc tcgtcaaggc
gaacaacgcc atgggatacg ccctcggctt 8100cctccagggc tacgacgtgg
cctggtggcg cgcgcgccat aacacgcacc acgtgtgcac 8160caacgaggat
ggttcggacc cggacatcaa gacggcgccc ctgctcatct acgtgcgaga
8220gaacccgtcc attgccaagc ggctcaactt cttccagcgc tggcagcagt
actactatgt 8280gccgaccatg gccatcctcg acctctactg gcgcctggag
tccatcgcgt acgtggctgt 8340gcgcctgcct aagatgtgga tgcaggccgc
cgctcttgcc gctcactacg cgctcctgtg 8400ctgggtcttc gcagcgcatc
tcaacctcat ccctctcatg atggttgcac gcggcttcgc 8460gacgggcatc
gttgtctttg caacccacta tggtgaggac atcctcgacc gcgagcacgt
8520cgagggcatg acgctcgtcg agcagaccgc caagacctcc cgtaacatca
cgggcggctg 8580gctagtgaac gtgctcacgg gcttcatctc cctgcagacc
gagcatcacc tcttccccat 8640gatgcccacc ggcaacctaa tgactatcca
gcccgaggta cgcgacttct tcaagaagca 8700tggcctcgag taccgcgagg
gcaacctctt ccagtgcgtg caccagaaca tcaaggctct 8760cgccttcgag
cacctcctcc actgagcggc cgcgacacaa gtgtgagagt actaaataaa
8820tgctttggtt gtacgaaatc attacactaa ataaaataat caaagcttat
atatgccttc 8880cgctaaggcc gaatgcaaag aaattggttc tttctcgtta
tcttttgcca cttttactag 8940tacgtattaa ttactactta atcatctttg
tttacggctc attatatccg gtctagagga 9000tccaaggccg cgaagttaaa
agcaatgttg tcacttgtcg tactaacaca tgatgtgata 9060gtttatgcta
gctagctata acataagctg tctctgagtg tgttgtatat taataaagat
9120catcactggt gaatggtgat cgtgtacgta ccctacttag taggcaatgg
aagcacttag 9180agtgtgcttt gtgcatggcc ttgcctctgt tttgagactt
ttgtaatgtt ttcgagttta 9240aatctttgcc tttgcgtacg tctagaggat ccccgg
92764511366DNAArtificial Sequenceplasmid pKR973 45ggagatccaa
gcttggcgcg ccgttctata gtgtcaccta aatcgtatgt gtatgataca 60taaggttatg
tattaattgt agccgcgttc taacgacaat atgtccatat ggtgcactct
120cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
caacacccgc 180tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
tacagacaag ctgtgaccgt 240ctccgggagc tgcatgtgtc agaggttttc
accgtcatca ccgaaacgcg cgagacgaaa 300gggcctcgtg atacgcctat
ttttataggt taatgtcatg accaaaatcc cttaacgtga 360gttttcgttc
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
420tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
cagcggtggt 480ttgtttgccg gatcaagagc taccaactct ttttccgaag
gtaactggct tcagcagagc 540gcagatacca aatactgtcc ttctagtgta
gccgtagtta ggccaccact tcaagaactc 600tgtagcaccg cctacatacc
tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 660cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
720gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
cctacaccga 780actgagatac ctacagcgtg agcattgaga aagcgccacg
cttcccgaag ggagaaaggc 840ggacaggtat ccggtaagcg gcagggtcgg
aacaggagag cgcacgaggg agcttccagg 900gggaaacgcc tggtatcttt
atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 960atttttgtga
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
1020tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
cgttatcccc 1080tgattctgtg gataaccgta ttaccgcctt tgagtgagct
gataccgctc gccgcagccg 1140aacgaccgag cgcagcgagt cagtgagcga
ggaagcggaa gagcgcccaa tacgcaaacc 1200gcctctcccc gcgcgttggc
cgattcatta atgcaggttg atcagatctc gatcccgcga 1260aattaatacg
actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
1320aactttaaga aggagatata cccatggaaa agcctgaact caccgcgacg
tctgtcgaga 1380agtttctgat cgaaaagttc gacagcgtct ccgacctgat
gcagctctcg gagggcgaag 1440aatctcgtgc tttcagcttc gatgtaggag
ggcgtggata tgtcctgcgg gtaaatagct 1500gcgccgatgg tttctacaaa
gatcgttatg tttatcggca ctttgcatcg gccgcgctcc 1560cgattccgga
agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
1620gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
gctgttctgc 1680agccggtcgc ggaggctatg gatgcgatcg ctgcggccga
tcttagccag acgagcgggt 1740tcggcccatt cggaccgcaa ggaatcggtc
aatacactac atggcgtgat ttcatatgcg 1800cgattgctga tccccatgtg
tatcactggc aaactgtgat ggacgacacc gtcagtgcgt 1860ccgtcgcgca
ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
1920acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
cgcataacag 1980cggtcattga ctggagcgag gcgatgttcg gggattccca
atacgaggtc gccaacatct 2040tcttctggag gccgtggttg gcttgtatgg
agcagcagac gcgctacttc gagcggaggc 2100atccggagct tgcaggatcg
ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc 2160aactctatca
gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
2220gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
gcccgcagaa 2280gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
cgatagtgga aaccgacgcc 2340ccagcactcg tccgagggca aaggaatagt
gaggtacagc ttggatcgat ccggctgcta 2400acaaagcccg aaaggaagct
gagttggctg ctgccaccgc tgagcaataa ctagcataac 2460cccttggggc
ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
2520gatgatcggg cgcgccgtcg acggatccgt acccggggat cctctagacg
tacgcaaagg 2580caaagattta aactcgaaaa cattacaaaa gtctcaaaac
agaggcaagg ccatgcacaa 2640agcacactct aagtgcttcc attgcctact
aagtagggta cgtacacgat caccattcac 2700cagtgatgat ctttattaat
atacaacaca ctcagagaca gcttatgtta tagctagcta 2760gcataaacta
tcacatcatg tgttagtacg acaagtgaca acattgcttt taacttcgcg
2820gccttggatc ctctagaccg gatataatga gccgtaaaca aagatgatta
agtagtaatt 2880aatacgtact agtaaaagtg gcaaaagata acgagaaaga
accaatttct ttgcattcgg 2940ccttagcgga aggcatatat aagctttgat
tattttattt agtgtaatga tttcgtacaa 3000ccaaagcatt tatttagtac
tctcacactt gtgtcgcggc cgctcagtgg aggaggtgct 3060cgaaggcgag
agccttgatg ttctggtgca cgcactggaa gaggttgccc tcgcggtact
3120cgaggccatg cttcttgaag aagtcgcgta cctcgggctg gatagtcatt
aggttgccgg 3180tgggcatcat ggggaagagg tgatgctcgg tctgcaggga
gatgaagccc gtgagcacgt 3240tcactagcca gccgcccgtg atgttacggg
aggtcttggc ggtctgctcg acgagcgtca 3300tgccctcgac gtgctcgcgg
tcgaggatgt cctcaccata gtgggttgca aagacaacga 3360tgcccgtcgc
gaagccgcgt gcaaccatca tgagagggat gaggttgaga tgcgctgcga
3420agacccagca caggagcgcg tagtgagcgg caagagcggc ggcctgcatc
cacatcttag 3480gcaggcgcac agccacgtac gcgatggact ccaggcgcca
gtagaggtcg aggatggcca 3540tggtcggcac atagtagtac tgctgccagc
gctggaagaa gttgagccgc ttggcaatgg 3600acgggttctc tcgcacgtag
atgagcaggg gcgccgtctt gatgtccggg tccgaaccat 3660cctcgttggt
gcacacgtgg tgcgtgttat ggcgcgcgcg ccaccaggcc acgtcgtagc
3720cctggaggaa gccgagggcg tatcccatgg cgttgttcgc cttgacgagc
gtgttgaccg 3780agcccttgaa gactgcgtga tgcaggtagt cgtgagcaag
ccagccgtcg aggtagaagc 3840caaagcccat ggagagcgcg cccgcgaggg
gcgagacgcc gctccgcacg agataggcgc 3900ccacgaagat gggcgcaagg
acgatggtga gcttgaacag ctcgtccaac gggcaagggt 3960cgtagagacc
agccctcttg tggcgctcaa tcatctcctt gaactccgtg cccatggtgg
4020accgcgaggg caggggcacg gaagcgggcg catcctccga cttcttgagc
atgccctcca 4080tcatcttgat ggccttgggc gagtggtaag actcgaactg
ctccgtcgcg tcgcgctcct 4140ggaagatgcg gaacaccttg ctcccgcccg
ggtgtgcctt ctggaacttt gtgacatcca 4200cgcgcttgcc gtagagcacg
acgtgcacgc tcttgctatc gacggagctc acctcagcga 4260gagacgcgtc
ggtcccgctc accgcctgcg cgccgccgtc tccacccttg cccatggtgc
4320ggccgcttgg ggggctatgg aagactttct tagttagttg tgtgaataag
caatgttggg 4380agaatcggga ctacttatag gataggaata aaacagaaaa
gtattaagtg ctaatgaaat 4440atttagactg ataattaaaa tcttcacgta
tgtccacttg atataaaaac gtcaggaata 4500aaggaagtac agtagaattt
aaaggtactc tttttatata tacccgtgtt ctctttttgg 4560ctagctagtt
gcataaaaaa taatctatat ttttatcatt attttaaata tcttatgaga
4620tggtaaatat ttatcataat tttttttact attatttatt atttgtgtgt
gtaatacata 4680tagaagttaa ttacaaattt tatttacttt ttcattattt
tgatatgatt caccattaat 4740ttagtgttat tatttataat agttcatttt
aatctttttg tatatattat gcgtgcagta 4800cttttttcct acatataact
actattacat tttatttata taatattttt attaatgaat 4860tttcgtgata
atatgtaata ttgttcatta ttatttcaga ttttttaaaa atatttgtgt
4920tattatttat gaaatatgta atttttttag tatttgattt tatgatgata
aagtgttcta 4980aattcaaaag aagggggaaa gcgtaaacat taaaaaacgt
catcaaacaa aaacaaaatc 5040ttgttaataa agataaaact gtttgttttg
atcactgtta tttcgtaata taaaaacatt 5100atttatattt atattgttga
caaccaaatt tgcctatcaa atctaaccaa tataatgcat 5160gcgtggcagg
taatgtacta ccatgaactt aagtcatgac ataataaacc gtgaatctga
5220ccaatgcatg tacctancta aattgtattt gtgacacgaa gcaaatgatt
caattcacaa 5280tggagatggg aaacaaataa tgaagaaccc agaactaaga
aagcttttct gaaaaataaa 5340ataaaggcaa tgtcaaaagt atactgcatc
atcagtccag aaagcacatg atattttttt 5400atcagtatca atgcagctag
ttttatttta caatatcgat atagctagtt taaatatatt 5460gcagctagat
ttataaatat ttgtgttatt atttatcatt tgtgtaatcc tgtttttagt
5520attttagttt atatatgatg ataatgtatt ccaaatttaa aagaagggaa
ataaatttaa 5580acaagaaaaa aagtcatcaa acaaaaaaca aatgaaaggg
tggaaagatg ttaccatgta 5640atgtgaatgt tacagtattt cttttattat
agagttaaca aattaactaa tatgattttg 5700ttaataatga taaaatattt
tttttattat tatttcataa tataaaaata gtttacttaa 5760tataaaaaaa
attctatcgt tcacaacaaa gttggccacc taatttaacc atgcatgtac
5820ccatggacca tattaggtaa ccatcaaacc tgatgaagag ataaagagat
gaagacttaa 5880gtcataacac aaaaccataa aaaacaaaaa tacaatcaac
cgtcaatctg accaatgcat 5940gaaaaagctg caatagtgag tggcgacaca
aagcacatga ttttcttaca acggagataa 6000aaccaaaaaa atatttcatg
aacaacctag aacaaataaa gcttttatat aataaatata 6060taaataaata
aaggctatgg aataatatac ttcaatatat ttggattaaa taaattgttg
6120gcggggttga tatatttata cacacctaaa gtcacttcaa tctcattttc
acttaacttt 6180tatttttttt ttctttttat ttatcataaa gagaatattg
ataatatact ttttaacata 6240tttttatgac attttttatt ggtgaaaact
tattaaaaat cataaatttt gtaagttaga 6300tttatttaaa gagttcctct
tcttatttta aattttttaa taaattttta aataactaaa 6360atttgtgtta
aaaatgttaa aaaatgtgtt attaaccctt ctcttcgagg acgtacgaag
6420cttagggcgg atcccccggg cgacctcgag ggggggcccg gtacgtgaag
gttaaacatg 6480gtgaatatgt taccactagc tgggatgccc attagatcaa
aactgtaaaa ttctcccgtt 6540tcccttctat tcacatgtga gccccctccc
ttttctttct ttctcaattt tgattgagtt 6600aaagtcacca gcaatgcatc
actcaccctc caaaaaattt cttgtacaac ttctcggact 6660atcccaaagc
tccttttcct gagatggatg gtcctgtctc ttgcccttga tgtcttcctt
6720gttcgatttt ggcttcctct aatgtctttc ttgctaggaa tcaccacctc
actcatctat 6780gttgtcgtag cttctgaaag tctcatacat atccttagtg
ttgcactcat cttgtattga 6840agtgaaaaag aatgttgttc tcctatccaa
atctccattg aatctctttc tcccaatgtt 6900gtcccatcgg ttggtcctcc
tctccaacca attgtaaggt gtttaacata aacatggtac 6960aattaagatt
tttcatttca ttaagaaaag attgagattt gtggttctaa agtttcaatt
7020agagtttgat gatattgaaa caaccgtaga acacattaag tattactaac
ttatacatag 7080agcattggaa tttcaccttt tatttattct gtttccgcca
aaggtacatg actcaagtta 7140ttttacacaa gtaacaaagg catctaagcc
taagtattct tattcagact tttcattatt 7200actttcattg atttggtgcg
aaatgcggcc gcctactctt ccttgggacg gagtccaaga 7260acacgcaagt
gctccaaatg tgaagcaaat gcttgccaaa acgtatcctt gacaaggtat
7320ggaaccttgt actcgctgca ggtgttcttg atgatggcca gaatatcggg
ataatggtgc 7380tgcgacacgt tggggaacag atggtgcaca gcctggtagt
tcaagctgcc agtgatgctg 7440gtccagaggt gcgaatcgtg tgcgtaatcc
tgcgtagtct cgacctgcat agctgcccag 7500tccttttgga tgatcccgtt
ctcgtcaggc aacggccact gaacttcctc aacaacgtgg 7560ttcgcctgga
aggtcagcgc cagccagtaa gacgacacca tgtccgcgac cgtgaacaag
7620agcagcacct tgcccagggg cagatactgc aggggaacaa tcaggcgata
ccagacaaag 7680aaagccttgc cgccccagaa catcacagtg tgccatgtcg
agatgggatt gacacgaata 7740gcgtcattgg tcttgacaaa gtacaaaatg
ttgatgtcct gaatgcgcac cttgaacgcc 7800agcagtccgt acaggaaagg
aacaaacatg tgctggttga tgtggttgac aaaccacttt 7860tggttgggct
tgatacgacg aacatcgggc tcagacgtcg acacgtcggg atctgctcca
7920gcaatgttgg tgtaggggtg atggccgagc atatgttggt acatccacac
caggtacgat 7980gctccgttga aaaagtcgtg cgtggctccc agaatcttcc
agacagtggg gttgtgggtc 8040actgaaaagt gagacgcatc atgaagaggg
ttgagtccga cttgtgcgca cgcaaatccc 8100atgatgattg caaacaccac
ctgaagccat gtgcgttcga caacgaaagg cacaaagagc 8160tgcgcgtagt
aggaagcgat caaggatcca aagataagag cgtatcgtcc ccagatctct
8220ggtctattct tgggatcaat gttccgatcc gtaaagtagc cctcgactct
cgtcttgatg 8280gttttgtgga acaccgttgg ctccgggaag atgggcagct
cattcgagac cagtgtaccg 8340acatagtact tcttcataat ggcatctgca
gccccaaacg cgtgatacat ctcaaagacc 8400ggagtaacat ctcggccagc
tccgagcagg agagtgtcca ctccaccagg atggcggctc 8460aagaactttg
tgacatcgta caccctgccg cggatggcca agagtaggtc gtccttggtg
8520ttatgggccg ccagctcttc ccaggtgaag gtttttcctt ggtccgttcc
catgcggccg 8580cggtgatgac tgatgagtgt ttaaggacca atggagagaa
tgtttgagtt gtgaagcgga 8640gaacctgagg cgtggttatt tatagggaag
agaggaaggt gaatgaggga cacgtcacag 8700aagtagggtg ctgagcttga
gacattcttc agtatgcatg gctatggaag ccttgggtgc 8760tacacctcat
gaagttcatg gtgtgaggtg gcttcggcat ctcaattaag tgacaaagag
8820aaaggtgttt cagtgtttct attgcaaatg gcagaaactc gtgatgacga
ggggaccatg 8880catggtttca tttcttttct tcctggattc tttctttcct
tttatatatg caggttcata 8940atttaaaaat tagactcgct ttcaatttct
taatttctca ttttcctctt atattactgt 9000actaatgtta accacgtaca
cttatttttt ttttagttta attttgatag attgtgttga 9060tttaaacata
ttaatatttt caaccaaata aaaatcattt tagtagatac ggctttttaa
9120ataattatta aaaatattaa ctatttatcc taaatggcac attttaatta
aaaaaaatcc 9180ggtgttgtaa gtgttttatt aatttgtttt ggcattatta
aagcaacttt ttttttattt 9240gttggcattt tgagtacgta cttaggctag
cctgcaggcc aactgcgttt ggggctccag 9300attaaacgac gccgtttcgt
tcctttcgct tcacggctta acgatgtcgt ttctgtctgt 9360gcccaaaaaa
taaaggcatt tgttatttgc accagatatt tactaagtgc accctagttt
9420gacaagtagg cgataattac aaatagatgc ggtgcaaata ataaattttg
aaggaaataa 9480ttacaaaaga acagaactta tatttacttt attttaaaaa
actaaaatga aagaacaaaa 9540aaagtaaaaa atacaaaaaa tgtgctttaa
ccactttcat tatttgttac agaaagtatg 9600attctactca aattgatctg
ttgtatctgg tgctgccttg tcacactggc gatttcaatc 9660ccctaaagat
atggtgcaaa ctgcgaagtg atcaatatct gctcggttaa tttagattaa
9720ttaataatat tcaacgtgat gtaccaaaaa aagacaattt tttgctccat
tgacaaatta 9780aacctcatca aggtaatttc caaacctata agcaaaaaaa
tttcacatta attggcccgc 9840aatcctatta gtcttattat actagagtag
gaaaaaaaac aattacacaa cttgtcttat 9900tattctctat gctaatgaat
atttttccct tttgttagaa atcagtgttt cctaatttat 9960tgagtattaa
ttccactcac cgcatatatt taccgttgaa taagaaaatt ttacacataa
10020ttctttttaa gataaataat ttttttatac tagatcttat atgattacgt
gaagccaagt 10080gggttatact aatgatatat aatgtttgat agtaatcagt
ttataaacca aatgcatgga 10140aatgttacgt ggaagcacgt aaattaacaa
gcattgaagc aaatgcagcc accgcaccaa 10200aaccacccca cttcacttcc
acgtaccata ttccatgcaa ctacaacacc ctaaaacttc 10260aataaatgcc
cccaccttca cttcacttca cccatcaata gcaagcggcc gcaccatgga
10320ggtggtgaat gaaatagtct caattgggca ggaagtttta cccaaagttg
attatgccca 10380actctggagt gatgccagtc actgtgaggt gctttacttg
tccatcgcat ttgtcatctt 10440gaagttcact cttggccccc ttggtccaaa
aggtcagtct cgtatgaagt ttgttttcac 10500caattacaac cttctcatgt
ccatttattc gttgggatca ttcctctcaa tggcatatgc 10560catgtacacc
atcggtgtta tgtctgacaa ctgcgagaag gcttttgaca acaacgtctt
10620caggatcacc acgcagttgt tctatttgag caagttcctg gagtatattg
actccttcta 10680tttgccactg atgggcaagc ctctgacctg gttgcaattc
ttccatcatt tgggggcacc 10740gatggatatg tggctgttct ataattaccg
aaatgaagct gtttggattt ttgtgctgtt 10800gaatggtttc atccactgga
tcatgtacgg ttattattgg accagattga tcaagctgaa 10860gttccccatg
ccaaaatccc tgattacatc aatgcagatc attcaattca atgttggttt
10920ctacattgtc tggaagtaca ggaacattcc ctgttatcgc caagatggga
tgaggatgtt 10980tggctggttc ttcaattact tttatgttgg cacagtcttg
tgtttgttct tgaatttcta 11040tgtgcaaacg tatatcgtca ggaagcacaa
gggagccaaa aagattcagt gagcggccgc 11100gaagttaaaa gcaatgttgt
cacttgtcgt actaacacat gatgtgatag tttatgctag 11160ctagctataa
cataagctgt ctctgagtgt gttgtatatt aataaagatc atcactggtg
11220aatggtgatc gtgtacgtac cctacttagt aggcaatgga agcacttaga
gtgtgctttg 11280tgcatggcct tgcctctgtt ttgagacttt tgtaatgttt
tcgagtttaa atctttgcct 11340ttgcgtacgt ctagagtcga cctgca
11366467085DNAArtificial Sequenceplasmid pKR72 46gtacggatcc
gtcgacggcg cgcccgatca tccggatata gttcctcctt tcagcaaaaa 60acccctcaag
acccgtttag aggccccaag gggttatgct agttattgct cagcggtggc
120agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatcgatcc
aagctgtacc 180tcactattcc tttgccctcg gacgagtgct ggggcgtcgg
tttccactat cggcgagtac 240ttctacacag ccatcggtcc agacggccgc
gcttctgcgg gcgatttgtg tacgcccgac 300agtcccggct ccggatcgga
cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc 360gaaattgccg
tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
420ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
gcgtctgctg 480ctccatacaa gccaaccacg gcctccagaa gaagatgttg
gcgacctcgt attgggaatc 540cccgaacatc gcctcgctcc agtcaatgac
cgctgttatg cggccattgt ccgtcaggac 600attgttggag ccgaaatccg
cgtgcacgag gtgccggact tcggggcagt cctcggccca 660aagcatcagc
tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
720ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
atgtagtgta 780ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
gtctggctaa gatcggccgc 840agcgatcgca tccatagcct ccgcgaccgg
ctgcagaaca gcgggcagtt cggtttcagg 900caggtcttgc aacgtgacac
cctgtgcacg gcgggagatg caataggtca ggctctcgct 960gaattcccca
atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
1020aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
catatccacg 1080ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
tccgagagct gcatcaggtc 1140ggagacgctg tcgaactttt cgatcagaaa
cttctcgaca gacgtcgcgg tgagttcagg 1200cttttccatg ggtatatctc
cttcttaaag ttaaacaaaa ttatttctag agggaaaccg 1260ttgtggtctc
cctatagtga gtcgtattaa tttcgcggga tcgagatcga tccaattcca
1320atcccacaaa aatctgagct taacagcaca gttgctcctc tcagagcaga
atcgggtatt 1380caacaccctc atatcaacta ctacgttgtg tataacggtc
cacatgccgg tatatacgat 1440gactggggtt gtacaaaggc ggcaacaaac
ggcgttcccg gagttgcaca caagaaattt 1500gccactatta cagaggcaag
agcagcagct gacgcgtaca caacaagtca gcaaacagac 1560aggttgaact
tcatccccaa aggagaagct caactcaagc ccaagagctt tgctaaggcc
1620ctaacaagcc caccaaagca aaaagcccac tggctcacgc taggaaccaa
aaggcccagc 1680agtgatccag ccccaaaaga gatctccttt gccccggaga
ttacaatgga cgatttcctc 1740tatctttacg atctaggaag gaagttcgaa
ggtgaaggtg acgacactat gttcaccact 1800gataatgaga aggttagcct
cttcaatttc agaaagaatg ctgacccaca gatggttaga 1860gaggcctacg
cagcaggtct catcaagacg atctacccga gtaacaatct ccaggagatc
1920aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
ttgcatcaag 1980aacacagaga aagacatatt tctcaagatc agaagtacta
ttccagtatg gacgattcaa 2040ggcttgcttc ataaaccaag gcaagtaata
gagattggag tctctaaaaa ggtagttcct 2100actgaatcta aggccatgca
tggagtctaa gattcaaatc gaggatctaa cagaactcgc 2160cgtgaagact
ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
2220cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca
aagatacagt 2280ctcagaagac caaagggcta
ttgagacttt tcaacaaagg ataatttcgg gaaacctcct 2340cggattccat
tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
2400ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatg
cctctgccga 2460cagtggtccc aaagatggac ccccacccac gaggagcatc
gtggaaaaag aagacgttcc 2520aaccacgtct tcaaagcaag tggattgatg
tgacatctcc actgacgtaa gggatgacgc 2580acaatcccac tatccttcgc
aagacccttc ctctatataa ggaagttcat ttcatttgga 2640gaggacacgc
tcgagctcat ttctctatta cttcagccat aacaaaagaa ctcttttctc
2700ttcttattaa accatgaaaa agcctgaact caccgcgacg tctgtcgaga
agtttctgat 2760cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
gagggcgaag aatctcgtgc 2820tttcagcttc gatgtaggag ggcgtggata
tgtcctgcgg gtaaatagct gcgccgatgg 2880tttctacaaa gatcgttatg
tttatcggca ctttgcatcg gccgcgctcc cgattccgga 2940agtgcttgac
attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
3000gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
agccggtcgc 3060ggaggccatg gatgcgatcg ctgcggccga tcttagccag
acgagcgggt tcggcccatt 3120cggaccgcaa ggaatcggtc aatacactac
atggcgtgat ttcatatgcg cgattgctga 3180tccccatgtg tatcactggc
aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 3240ggctctcgat
gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
3300cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
cggtcattga 3360ctggagcgag gcgatgttcg gggattccca atacgaggtc
gccaacatct tcttctggag 3420gccgtggttg gcttgtatgg agcagcagac
gcgctacttc gagcggaggc atccggagct 3480tgcaggatcg ccgcggctcc
gggcgtatat gctccgcatt ggtcttgacc aactctatca 3540gagcttggtt
gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
3600cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
gcgcggccgt 3660ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
aaccgacgcc ccagcactcg 3720tccgagggca aaggaatagt gaggtaccta
aagaaggagt gcgtcgaagc agatcgttca 3780aacatttggc aataaagttt
cttaagattg aatcctgttg ccggtcttgc gatgattatc 3840atataatttc
tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
3900tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
cgcgatagaa 3960aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
cggtgtcatc tatgttacta 4020gatcgatgtc gaatcgatca acctgcatta
atgaatcggc caacgcgcgg ggagaggcgg 4080tttgcgtatt gggcgctctt
ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4140gctgcggcga
gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4200ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
accgtaaaaa 4260ggccgcgttg ctggcgtttt tccataggct ccgcccccct
gacgagcatc acaaaaatcg 4320acgctcaagt cagaggtggc gaaacccgac
aggactataa agataccagg cgtttccccc 4380tggaagctcc ctcgtgcgct
ctcctgttcc gaccctgccg cttaccggat acctgtccgc 4440ctttctccct
tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
4500ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
agcccgaccg 4560ctgcgcctta tccggtaact atcgtcttga gtccaacccg
gtaagacacg acttatcgcc 4620actggcagca gccactggta acaggattag
cagagcgagg tatgtaggcg gtgctacaga 4680gttcttgaag tggtggccta
actacggcta cactagaagg acagtatttg gtatctgcgc 4740tctgctgaag
ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4800caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
gaaaaaaagg 4860atctcaagaa gatcctttga tcttttctac ggggtctgac
gctcagtgga acgaaaactc 4920acgttaaggg attttggtca tgacattaac
ctataaaaat aggcgtatca cgaggccctt 4980tcgtctcgcg cgtttcggtg
atgacggtga aaacctctga cacatgcagc tcccggagac 5040ggtcacagct
tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
5100gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
ttgtactgag 5160agtgcaccat atggacatat tgtcgttaga acgcggctac
aattaataca taaccttatg 5220tatcatacac atacgattta ggtgacacta
tagaacggcg cgccaagctt gttgaaacat 5280ccctgaagtg tctcatttta
ttttatttat tctttgctga taaaaaaata aaataaaaga 5340agctaagcac
acggtcaacc attgctctac tgctaaaagg gttatgtgta gtgttttact
5400gcataaatta tgcagcaaac aagacaactc aaattaaaaa atttcctttg
cttgtttttt 5460tgttgtctct gacttgactt tcttgtggaa gttggttgta
taaggattgg gacaccattg 5520tccttcttaa tttaatttta ttctttgctg
ataaaaaaaa aaatttcata tagtgttaaa 5580taataatttg ttaaataacc
aaaaagtcaa atatgtttac tctcgtttaa ataattgaga 5640ttcgtccagc
aaggctaaac gattgtatag atttatgaca atatttactt ttttatagat
5700aaatgttata ttataataaa tttatataca tatattatat gttatttatt
attattttaa 5760atccttcaat attttatcaa accaactcat aatttttttt
ttatctgtaa gaagcaataa 5820aattaaatag acccacttta aggatgatcc
aacctttata cagagtaaga gagttcaaat 5880agtacccttt catatacata
tcaactaaaa tattagaaat atcatggatc aaaccttata 5940aagacattaa
ataagtggat aagtataata tataaatggg tagtatataa tatataaatg
6000gatacaaact tctctcttta taattgttat gtctccttaa catcctaata
taatacataa 6060gtgggtaata tataatatat aaatggagac aaacttcttc
cattataatt gttatgtctt 6120cttaacactt atgtctcgtt cacaatgcta
aggttagaat tgtttagaaa gtcttatagt 6180acacatttgt ttttgtacta
tttgaagcat tccataagcc gtcacgattc agatgattta 6240taataataag
aggaaattta tcatagaaca ataaggtgca tagatagagt gttaatatat
6300cataacatcc tttgtttatt catagaagaa gtgagatgga gctcagttat
tatactgtta 6360catggtcgga tacaatattc catgctctcc atgagctctt
acacctacat gcattttagt 6420tcatacttgc ggccgcagta tatcttaaat
tctttaatac ggtgtactag gatattgaac 6480tggttcttga tgatgaaaac
ctgggccgag attgcagcta tttatagtca taggtcttgt 6540taacatgcat
ggacatttgg ccacggggtg gcatgcagtt tgacgggtgt tgaaataaac
6600aaaaatgagg tggcggaaga gaatacgagt ttgaggttgg gttagaaaca
acaaatgtga 6660gggctcatga tgggttgagt tggtgaatgt tttgggctgc
tcgattgaca cctttgtgag 6720tacgtgttgt tgtgcatggc ttttggggtc
cagttttttt ttcttgacgc ggcgatcctg 6780atcagctagt ggataagtga
tgtccactgt gtgtgattgc gtttttgttt gaattttatg 6840aacttagaca
ttgctatgca aaggatactc tcattgtgtt ttgtcttctt ttgttccttg
6900gctttttctt atgatccaag agactagtca gtgttgtggc attcgagact
accaagatta 6960attatgatgg gggaaggata agtaactgat tagtacggac
tgttaccaaa ttaattaata 7020agcggcaaat gaagggcatg gatcaaaagc
ttggatctcc tgcaggatct ggccggccgg 7080atctc 7085477873DNAArtificial
Sequenceplasmid pKR912 47ggccgcaagt atgaactaaa atgcatgtag
gtgtaagagc tcatggagag catggaatat 60tgtatccgac catgtaacag tataataact
gagctccatc tcacttcttc tatgaataaa 120caaaggatgt tatgatatat
taacactcta tctatgcacc ttattgttct atgataaatt 180tcctcttatt
attataaatc atctgaatcg tgacggctta tggaatgctt caaatagtac
240aaaaacaaat gtgtactata agactttcta aacaattcta accttagcat
tgtgaacgag 300acataagtgt taagaagaca taacaattat aatggaagaa
gtttgtctcc atttatatat 360tatatattac ccacttatgt attatattag
gatgttaagg agacataaca attataaaga 420gagaagtttg tatccattta
tatattatat actacccatt tatatattat acttatccac 480ttatttaatg
tctttataag gtttgatcca tgatatttct aatattttag ttgatatgta
540tatgaaaggg tactatttga actctcttac tctgtataaa ggttggatca
tccttaaagt 600gggtctattt aattttattg cttcttacag ataaaaaaaa
aattatgagt tggtttgata 660aaatattgaa ggatttaaaa taataataaa
taacatataa tatatgtata taaatttatt 720ataatataac atttatctat
aaaaaagtaa atattgtcat aaatctatac aatcgtttag 780ccttgctgga
cgaatctcaa ttatttaaac gagagtaaac atatttgact ttttggttat
840ttaacaaatt attatttaac actatatgaa attttttttt ttatcagcaa
agaataaaat 900taaattaaga aggacaatgg tgtcccaatc cttatacaac
caacttccac aagaaagtca 960agtcagagac aacaaaaaaa caagcaaagg
aaatttttta atttgagttg tcttgtttgc 1020tgcataattt atgcagtaaa
acactacaca taaccctttt agcagtagag caatggttga 1080ccgtgtgctt
agcttctttt attttatttt tttatcagca aagaataaat aaaataaaat
1140gagacacttc agggatgttt caacaagctt ggcgcgccgt tctatagtgt
cacctaaatc 1200gtatgtgtat gatacataag gttatgtatt aattgtagcc
gcgttctaac gacaatatgt 1260ccatatggtg cactctcagt acaatctgct
ctgatgccgc atagttaagc cagccccgac 1320acccgccaac acccgctgac
gcgccctgac gggcttgtct gctcccggca tccgcttaca 1380gacaagctgt
gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
1440aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
gtcatgacca 1500aaatccctta acgtgagttt tcgttccact gagcgtcaga
ccccgtagaa aagatcaaag 1560gatcttcttg agatcctttt tttctgcgcg
taatctgctg cttgcaaaca aaaaaaccac 1620cgctaccagc ggtggtttgt
ttgccggatc aagagctacc aactcttttt ccgaaggtaa 1680ctggcttcag
cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
1740accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
ctgttaccag 1800tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
ggactcaaga cgatagttac 1860cggataaggc gcagcggtcg ggctgaacgg
ggggttcgtg cacacagccc agcttggagc 1920gaacgaccta caccgaactg
agatacctac agcgtgagca ttgagaaagc gccacgcttc 1980ccgaagggag
aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
2040cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
tttcgccacc 2100tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
gcggagccta tggaaaaacg 2160ccagcaacgc ggccttttta cggttcctgg
ccttttgctg gccttttgct cacatgttct 2220ttcctgcgtt atcccctgat
tctgtggata accgtattac cgcctttgag tgagctgata 2280ccgctcgccg
cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
2340gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
aggttgatcg 2400attcgacatc gatctagtaa catagatgac accgcgcgcg
ataatttatc ctagtttgcg 2460cgctatattt tgttttctat cgcgtattaa
atgtataatt gcgggactct aatcataaaa 2520acccatctca taaataacgt
catgcattac atgttaatta ttacatgctt aacgtaattc 2580aacagaaatt
atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
2640tattgccaaa tgtttgaacg atctgcttcg acgcactcct tctttaggta
cctcactatt 2700cctttgccct cggacgagtg ctggggcgtc ggtttccact
atcggcgagt acttctacac 2760agccatcggt ccagacggcc gcgcttctgc
gggcgatttg tgtacgcccg acagtcccgg 2820ctccggatcg gacgattgcg
tcgcatcgac cctgcgccca agctgcatca tcgaaattgc 2880cgtcaaccaa
gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
2940gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
tgctccatac 3000aagccaacca cggcctccag aagaagatgt tggcgacctc
gtattgggaa tccccgaaca 3060tcgcctcgct ccagtcaatg accgctgtta
tgcggccatt gtccgtcagg acattgttgg 3120agccgaaatc cgcgtgcacg
aggtgccgga cttcggggca gtcctcggcc caaagcatca 3180gctcatcgag
agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
3240gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
tattgaccga 3300ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
aagatcggcc gcagcgatcg 3360catccatggc ctccgcgacc ggctgcagaa
cagcgggcag ttcggtttca ggcaggtctt 3420gcaacgtgac accctgtgca
cggcgggaga tgcaataggt caggctctcg ctgaattccc 3480caatgtcaag
cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
3540gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
cgccctccta 3600catcgaagct gaaagcacga gattcttcgc cctccgagag
ctgcatcagg tcggagacgc 3660tgtcgaactt ttcgatcaga aacttctcga
cagacgtcgc ggtgagttca ggctttttca 3720tggtttaata agaagagaaa
agagttcttt tgttatggct gaagtaatag agaaatgagc 3780tcgagcgtgt
cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
3840gatagtggga ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa
tccacttgct 3900ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
cctcgtgggt gggggtccat 3960ctttgggacc actgtcggca gaggcatctt
gaatgatagc ctttccttta tcgcaatgat 4020ggcatttgta ggagccacct
tccttttcta ctgtcctttc gatgaagtga cagatagctg 4080ggcaatggaa
tccgaggagg tttcccgaaa ttatcctttg ttgaaaagtc tcaatagccc
4140tttggtcttc tgagactgta tctttgacat ttttggagta gaccagagtg
tcgtgctcca 4200ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa
aagactctgt atgaactgtt 4260cgccagtctt cacggcgagt tctgttagat
cctcgatttg aatcttagac tccatgcatg 4320gccttagatt cagtaggaac
taccttttta gagactccaa tctctattac ttgccttggt 4380ttatgaagca
agccttgaat cgtccatact ggaatagtac ttctgatctt gagaaatatg
4440tctttctctg tgttcttgat gcaattagtc ctgaatcttt tgactgcatc
tttaaccttc 4500ttgggaaggt atttgatctc ctggagattg ttactcgggt
agatcgtctt gatgagacct 4560gctgcgtagg cctctctaac catctgtggg
tcagcattct ttctgaaatt gaagaggcta 4620accttctcat tatcagtggt
gaacatagtg tcgtcacctt caccttcgaa cttccttcct 4680agatcgtaaa
gatagaggaa atcgtccatt gtaatctccg gggcaaagga gatctctttt
4740ggggctggat cactgctggg ccttttggtt cctagcgtga gccagtgggc
tttttgcttt 4800ggtgggcttg ttagggcctt agcaaagctc ttgggcttga
gttgagcttc tcctttgggg 4860atgaagttca acctgtctgt ttgctgactt
gttgtgtacg cgtcagctgc tgctcttgcc 4920tctgtaatag tggcaaattt
cttgtgtgca actccgggaa cgccgtttgt tgccgccttt 4980gtacaacccc
agtcatcgta tataccggca tgtggaccgt tatacacaac gtagtagttg
5040atatgagggt gttgaatacc cgattctgct ctgagaggag caactgtgct
gttaagctca 5100gatttttgtg ggattggaat tggatcgatc tcgatcccgc
gaaattaata cgactcacta 5160tagggagacc acaacggttt ccctctagaa
ataattttgt ttaactttaa gaaggagata 5220tacccatgga aaagcctgaa
ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt 5280tcgacagcgt
ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
5340tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
ggtttctaca 5400aagatcgtta tgtttatcgg cactttgcat cggccgcgct
cccgattccg gaagtgcttg 5460acattgggga attcagcgag agcctgacct
attgcatctc ccgccgtgca cagggtgtca 5520cgttgcaaga cctgcctgaa
accgaactgc ccgctgttct gcagccggtc gcggaggcta 5580tggatgcgat
cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
5640aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
gatccccatg 5700tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
gtccgtcgcg caggctctcg 5760atgagctgat gctttgggcc gaggactgcc
ccgaagtccg gcacctcgtg cacgcggatt 5820tcggctccaa caatgtcctg
acggacaatg gccgcataac agcggtcatt gactggagcg 5880aggcgatgtt
cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
5940tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
cttgcaggat 6000cgccgcggct ccgggcgtat atgctccgca ttggtcttga
ccaactctat cagagcttgg 6060ttgacggcaa tttcgatgat gcagcttggg
cgcagggtcg atgcgacgca atcgtccgat 6120ccggagccgg gactgtcggg
cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg 6180atggctgtgt
agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
6240caaaggaata gtgaggtaca gcttggatcg atccggctgc taacaaagcc
cgaaaggaag 6300ctgagttggc tgctgccacc gctgagcaat aactagcata
accccttggg gcctctaaac 6360gggtcttgag gggttttttg ctgaaaggag
gaactatatc cggatgatcg ggcgcgccgt 6420cgacggatcc gtacgagatc
cggccggcca gatcctgcag gagatccaag cttttgatcc 6480atgcccttca
tttgccgctt attaattaat ttggtaacag tccgtactaa tcagttactt
6540atccttcccc catcataatt aatcttggta gtctcgaatg ccacaacact
gactagtctc 6600ttggatcata agaaaaagcc aaggaacaaa agaagacaaa
acacaatgag agtatccttt 6660gcatagcaat gtctaagttc ataaaattca
aacaaaaacg caatcacaca cagtggacat 6720cacttatcca ctagctgatc
aggatcgccg cgtcaagaaa aaaaaactgg accccaaaag 6780ccatgcacaa
caacacgtac tcacaaaggt gtcaatcgag cagcccaaaa cattcaccaa
6840ctcaacccat catgagccct cacatttgtt gtttctaacc caacctcaaa
ctcgtattct 6900cttccgccac ctcatttttg tttatttcaa cacccgtcaa
actgcatgcc accccgtggc 6960caaatgtcca tgcatgttaa caagacctat
gactataaat agctgcaatc tcggcccagg 7020ttttcatcat caagaaccag
ttcaatatcc tagtacaccg tattaaagaa tttaagatat 7080actgcggccg
caccatggag gtggtgaatg aaatagtctc aattgggcag gaagttttac
7140ccaaagttga ttatgcccaa ctctggagtg atgccagtca ctgtgaggtg
ctttacttgt 7200ccatcgcatt tgtcatcttg aagttcactc ttggccccct
tggtccaaaa ggtcagtctc 7260gtatgaagtt tgttttcacc aattacaacc
ttctcatgtc catttattcg ttgggatcat 7320tcctctcaat ggcatatgcc
atgtacacca tcggtgttat gtctgacaac tgcgagaagg 7380cttttgacaa
caacgtcttc aggatcacca cgcagttgtt ctatttgagc aagttcctgg
7440agtatattga ctccttctat ttgccactga tgggcaagcc tctgacctgg
ttgcaattct 7500tccatcattt gggggcaccg atggatatgt ggctgttcta
taattaccga aatgaagctg 7560tttggatttt tgtgctgttg aatggtttca
tccactggat catgtacggt tattattgga 7620ccagattgat caagctgaag
ttccccatgc caaaatccct gattacatca atgcagatca 7680ttcaattcaa
tgttggtttc tacattgtct ggaagtacag gaacattccc tgttatcgcc
7740aagatgggat gaggatgttt ggctggttct tcaattactt ttatgttggc
acagtcttgt 7800gtttgttctt gaatttctat gtgcaaacgt atatcgtcag
gaagcacaag ggagccaaaa 7860agattcagtg agc 7873489892DNAArtificial
Sequenceplasmid pKR886r 48ggtcgactcg acgtacgtct agaggatccg
tcgacggcgc gcccgatcat ccggatatag 60ttcctccttt cagcaaaaaa cccctcaaga
cccgtttaga ggccccaagg ggttatgcta 120gttattgctc agcggtggca
gcagccaact cagcttcctt tcgggctttg ttagcagccg 180gatcgatcca
agctgtacct cactattcct ttgccctcgg acgagtgctg gggcgtcggt
240ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
cttctgcggg 300cgatttgtgt acgcccgaca gtcccggctc cggatcggac
gattgcgtcg catcgaccct 360gcgcccaagc tgcatcatcg aaattgccgt
caaccaagct ctgatagagt tggtcaagac 420caatgcggag catatacgcc
cggagccgcg gcgatcctgc aagctccgga tgcctccgct 480cgaagtagcg
cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
540cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
gctgttatgc 600ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
gtgcacgagg tgccggactt 660cggggcagtc ctcggcccaa agcatcagct
catcgagagc ctgcgcgacg gacgcactga 720cggtgtcgtc catcacagtt
tgccagtgat acacatgggg atcagcaatc gcgcatatga 780aatcacgcca
tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
840tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggc
tgcagaacag 900cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
ctgtgcacgg cgggagatgc 960aataggtcag gctctcgctg aattccccaa
tgtcaagcac ttccggaatc gggagcgcgg 1020ccgatgcaaa gtgccgataa
acataacgat ctttgtagaa accatcggcg cagctattta 1080cccgcaggac
atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
1140ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
ttctcgacag 1200acgtcgcggt gagttcaggc ttttccatgg gtatatctcc
ttcttaaagt taaacaaaat 1260tatttctaga gggaaaccgt tgtggtctcc
ctatagtgag tcgtattaat ttcgcgggat 1320cgagatctga tcaacctgca
ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 1380attgggcgct
cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
1440cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
aggggataac 1500gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
ggaaccgtaa aaaggccgcg 1560ttgctggcgt ttttccatag gctccgcccc
cctgacgagc atcacaaaaa tcgacgctca 1620agtcagaggt ggcgaaaccc
gacaggacta taaagatacc aggcgtttcc ccctggaagc 1680tccctcgtgc
gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
1740ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
ttcggtgtag 1800gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
ttcagcccga ccgctgcgcc 1860ttatccggta actatcgtct tgagtccaac
ccggtaagac acgacttatc gccactggca 1920gcagccactg gtaacaggat
tagcagagcg aggtatgtag gcggtgctac agagttcttg 1980aagtggtggc
ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
2040aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
aaccaccgct 2100ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
gcagaaaaaa aggatctcaa 2160gaagatcctt tgatcttttc tacggggtct
gacgctcagt ggaacgaaaa ctcacgttaa
2220gggattttgg tcatgacatt aacctataaa aataggcgta tcacgaggcc
ctttcgtctc 2280gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
agctcccgga gacggtcaca 2340gcttgtctgt aagcggatgc cgggagcaga
caagcccgtc agggcgcgtc agcgggtgtt 2400ggcgggtgtc ggggctggct
taactatgcg gcatcagagc agattgtact gagagtgcac 2460catatggaca
tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
2520cacatacgat ttaggtgaca ctatagaacg gcgcgccaag ctgggtctag
aactagaaac 2580gtgatgccac ttgttattga agtcgattac agcatctatt
ctgttttact atttataact 2640ttgccatttc tgacttttga aaactatctc
tggatttcgg tatcgctttg tgaagatcga 2700gcaaaagaga cgttttgtgg
acgcaatggt ccaaatccgt tctacatgaa caaattggtc 2760acaatttcca
ctaaaagtaa ataaatggca agttaaaaaa ggaatatgca ttttactgat
2820tgcctaggtg agctccaaga gaagttgaat ctacacgtct accaaccgct
aaaaaaagaa 2880aaacattgat atgtaacctg attccattag cttttgactt
cttcaacaga ttctctactt 2940agatttctaa cagaaatatt attactagca
catcattttc agtctcacta cagcaaaaaa 3000tccaacggca caatacagac
aacaggagat atcagactac agagatagat agatgctact 3060gcatgtagta
agttaaataa aaggaaaata aaatgtcttg ctaccaaaac tactacagac
3120tatgatgctc accacaggcc aaatcctgca actaggacag cattatctta
tatatattgt 3180acaaaacaag catcaaggaa catttggtct aggcaatcag
tacctcgttc taccatcacc 3240ctcagttatc acatccttga aggatccatt
actgggaatc atcggcaaca catgctcctg 3300atggggcaca atgacatcaa
gaaggtaggg gccaggggtg tccaacattc tctgaattgc 3360cgctctaagc
tcttccttct tcgtcactcg cgctgccggt atcccacaag catcagcaaa
3420cttgagcatg tttgggaata tctcgctctc gctagacgga tctccaagat
aggtgtgagc 3480tctattggac ttgtagaacc tatcctccaa ctgaaccacc
atacccaaat gctgattgtt 3540caacaacaat atcttaactg ggagattctc
cactcttata gtggccaact cctgaacatt 3600catgatgaaa ctaccatccc
catcaatgtc aaccacaaca gccccagggt tagcaacagc 3660agcaccaata
gccgcaggca atccaaaacc catggctcca agaccccctg aggtcaacca
3720ctgcctcggt ctcttgtact tgtaaaactg cgcagcccac atttgatgct
gcccaacccc 3780agtactaaca atagcatctc cattagtcaa ctcatcaaga
acctcgatag catgctgcgg 3840agaaatcgcg tcctggaatg tcttgtaacc
caatggaaac ttgtgtttct gcacattaat 3900ctcttctctc caacctccaa
gatcaaactt accctccact cctttctcct ccaaaatcat 3960attaattccc
ttcaaggcca acttcaaatc cgcgcaaacc gacacgtgcg cctgcttgtt
4020cttcccaatc tcggcagaat caatatcaat gtgaacaatc ttagccctac
tagcaaaagc 4080ctcaagcttc ccagtaacac ggtcatcaaa ccttacccca
aaggcaagca acaaatcact 4140attgtcaaca gcatagttag cataaacagt
accatgcata cccagcatct gaagggaata 4200ttcatcacca ataggaaaag
ttccaagacc cattaaagtg ctagcaacgg gaataccagt 4260gagttcaaca
aagcgcctca attcagcact ggaattcaaa ctgccaccgc cgacgtagag
4320aacgggcttt tgggcctcca tgatgagtct gacaatgtgt tccaattggg
cctcggcggg 4380gggcctgggc agcctggcga ggtaaccggg gaggttaacg
ggctcgtccc aattaggcac 4440ggcgagttgc tgctgaacgt ctttgggaat
gtcgatgagg accggaccgg ggcggccgga 4500ggtggcgacg aagaaagcct
cggcgacgac gcgggggatg tcgtcgacgt cgaggatgag 4560gtagttgtgc
ttcgtgatgg atctgctcac ctccacgatc ggggtttctt ggaaggcgtc
4620ggtgccgatc atccggcggg cgacctggcc ggtgatggcg acgactggga
cgctgtccat 4680taaagcgtcg gcgaggccgc tcacgaggtt ggtggcgccg
gggccggagg tggcaatgca 4740gacgccgggg aggccggagg aacgcgcgta
gccttcggcg gcgaagacgc cgccctgctc 4800gtggcgcggg agcacgttgc
ggatggcggc ggagcgcgtg agcgcctggt ggatctccat 4860cgacgcaccg
ccggggtacg cgaacaccgt cgtcacgccc tgcctctcca gcgcctccac
4920aaggatgtcc gcgcccttgc gaggttcgcc ggaggcgaac cgtgacacga
agggctccgt 4980ggtcggcgct tccttggtga agggcgccgc cgtggggggt
ttggagatgg aacatttgat 5040tttgagagcg tggttgggtt tggtgagggt
ttgatgagag agagggaggg tggatctagt 5100aatgcgtttg gggaaggtgg
ggtgtgaaga ggaagaagag aatcgggtgg ttctggaagc 5160ggtggccgcc
attgtgttgt gtggcatggt tatacttcaa aaactgcaca acaagcctag
5220agttagtacc taaacagtaa atttacaaca gagagcaaag acacatgcaa
aaatttcagc 5280cataaaaaaa gttataatag aatttaaagc aaaagtttca
ttttttaaac atatatacaa 5340acaaactgga tttgaaggaa gggattaatt
cccctgctca aagtttgaat tcctattgtg 5400acctatactc gaataaaatt
gaagcctaag gaatgtatga gaaacaagaa aacaaaacaa 5460aactacagac
aaacaagtac aattacaaaa ttcgctaaaa ttctgtaatc accaaacccc
5520atctcagtca gcacaaggcc caaggtttat tttgaaataa aaaaaaagtg
attttatttc 5580tcataagcta aaagaaagaa aggcaattat gaaatgattt
cgactagatc tgaaagtcca 5640acgcgtattc cgcagatatt aaagaaagag
tagagtttca catggatcct agatggaccc 5700agttgaggaa aaagcaaggc
aaagcaaacc agaagtgcaa gatccgaaat tgaaccacgg 5760aatctaggat
ttggtagagg gagaagaaaa gtaccttgag aggtagaaga gaagagaaga
5820gcagagagat atatgaacga gtgtgtcttg gtctcaactc tgaagcgata
cgagtttaga 5880ggggagcatt gagttccaat ttatagggaa accgggtggc
aggggtgagt taatgacgga 5940aaagccccta agtaacgaga ttggattgtg
ggttagattc aaccgtttgc atccgcggct 6000tagattgggg aagtcagagt
gaatctcaac cgttgactga gttgaaaatt gaatgtagca 6060accaattgag
ccaaccccag cctttgccct ttgattttga tttgtttgtt gcatactttt
6120tatttgtctt ctggttctga ctctctttct ctcgtttcaa tgccaggttg
cctactccca 6180caccactcac aagaagattc tactgttagt attaaatatt
ttttaatgta ttaaatgatg 6240aatgcttttg taaacagaac aagactatgt
ctaataagtg tcttgcaaca ttttttaaga 6300aattaaaaaa aatatattta
ttatcaaaat caaatgtatg aaaaatcatg aataatataa 6360ttttatacat
ttttttaaaa aatcttttaa tttcttaatt aatatcttaa aaataatgat
6420taatatttaa cccaaaataa ttagtatgat tggtaaggaa gatatccatg
ttatgtttgg 6480atgtgagttt gatctagagc aaagcttact agagtcgacc
tgcagcccgg gggatccgcc 6540cacgtacggt accatctgct aatattttaa
atcacatgca agagaggagg catggttcca 6600ttttctacct tcacattatt
tgagaaaaac gaacttgttc tgtgttttat ttttgccctt 6660cacattagta
caacgtggaa gactcatggt tacacagaat catacataag tacaatgctt
6720gtccctaaga aaacaagcac tcgttgtatt gaacctttac ggctcatgcg
gccgcgaatt 6780cactagtgat tgaattcgcg gccgcttagt ccgacttggc
cttggcggcc gcggccgact 6840ctttgagcgt gaagatctgc gccgtctcgg
gcacagcgcc gtagttgaca aagaggtgcg 6900cggtcttgaa gaaggccgtg
atgatgggct cgtcgttcct gcgcacgagg tgcgggtacg 6960cggccgcaaa
gtgcttggtg gcttcgttga gcttgtagtg cggaatgatc gggaacaagt
7020ggtggacctg gtgcgtgcca atgtggtggc tcaggttgtc cacgaacgcg
ccgtacgagc 7080ggtcgacgct cgagaggttg cccttgacgt acgtccactc
cgagtcgccg taccacggcg 7140tcgcttcgtc gttgtggtgc aagaaggtcg
taatgacgag gaacgaagca aagacaaaga 7200gcggcgcata gtagtagagg
cccatgacgg caaagccgag cgagtatgtg aggtacgcgt 7260acgcggcgaa
gaaggcggcc cagacgccga gcgacacgat gacggccgac gcgcggcgaa
7320ggaggagcgg gtcccacggg tcaaagtggc tcatcgtgcg cggggcatac
ccgaccttca 7380agtagacaaa ccacgcaccg ccgagcgtgt agacccattg
gcgcacgtcc tggaggtcct 7440tgaccgaccg gtgcgggtaa aagatctcgt
ccttatcaat gttgcccgtg ttcttgtggt 7500ggtggcggtg cgtcacgcgc
cagctctcga acggcgtcaa aatcgcagag tgcatgatgc 7560agccgatgat
aaagttgacg ctgtggtagc gcgagaaggc cgagtggccg cagtcgtggc
7620cgaccgtgaa gaagccccag aagatgacgc cctgcacgta gatgtaggtg
gcgcaaacga 7680gcgcgtggag cagaacgtta tcggcaatga acggcgtcga
gcgcgccgcg tagagcagcg 7740ccgccgaggc cgacgcgttg aagatcgcgc
gggccgtgta gtagagcgag aggccgaggt 7800tcgactcaaa gcacgcgttc
gggatcgagt gcttgagctc cgtgagcgtc gggaactcga 7860ccttcgtctt
atcctcagtc atgcggccgc tgaagtattg cttcttagtt aacctttcct
7920ttctctctca gctatgtgaa ttcattttgc tttcgtcaca atttatatag
tgaaattgga 7980tctttggagt taacgccttc acaggattat cgtgttagaa
caatgctttt tcatgttcta 8040attagtagta cattacaaat gtgcactcta
ttcaataagc atcttttggc acgttaataa 8100atcatgtgaa aaaaaaatac
tactatttca aagaaagtgt tgtaaaaaga aacggaaaga 8160gagctggctt
cagttgttga gacttgtttg ctagtaaaaa tggtgtgaag agtgattcat
8220ggtgaggtgg tttttcgtcc ctttctgttt gcatgaaaaa caaatggcaa
gagatgacgt 8280aggattcctt cccttaacga ttatctgttt ttaatttcaa
atatacatat aggaatttat 8340gaattactaa ggttgtaaaa tatgctggtc
atttatttat ggctaaaata tttttttttc 8400tcgtaaatat aaaaatattt
aaaatttatt tttatcatat tttttatcct tataaaatta 8460tgtgtacaac
ctatataaaa aaatatcata tttaatattg attatatgtt taatcaatat
8520aaaaaatcat tatcatatat ttagatttat tcgaatatac atctaaacaa
aaaataacat 8580attttaattt tatgaagaaa aaaaaatatt ttatccttta
tttatttaag attaattaat 8640agttatgtat tgtggaaaga cttttacaca
tgcaatagat atactgaatc aattagatgc 8700caatgctgag ttggaaatca
cttgaggagg ggaggagact tgccaatgct tttcagtttc 8760atttaaatga
tttagtggag gagatagagt agtgataaag gcatgcccca attttggagt
8820gtatatatga gtggaaataa gagagggata gagagaaaaa ataaagagag
taaaaataat 8880taatgtgaaa tgatatgata aaaaaataaa gaaagagata
aagagaaaaa tgaaatgaga 8940gatagatgaa atagagagta gatacatgtt
tgtttaggtt ttttttagga aataacacat 9000ttttttctca tcacttatta
ctcactgtca atttcctctc tttcaatcat aatgatatga 9060tttgtttaac
aaaaatgtga aaaaacatat aaagtaaaat atttttataa attgataaat
9120aaaaatttac aaaatttatt tcttattaaa ttgaatagaa aatgaaagaa
aagaaaagaa 9180aaagtatata taaaatgata tagctttaaa aagaataaat
ttttcatatc agtctttttt 9240taataattta gaaatattta agtatatagc
aaaaatataa tgtactttac atatgcataa 9300ataataattt gaaaatagaa
ctaatagaat agagaaaaaa gtaatataat aattaactat 9360atgaaaattt
agaagggaca atatttttaa ttaagaatat aaacaatatt tcttttcatg
9420taatgaggga cggatgtacg gggccagtgt tggagtcaaa gccaaaatag
tcacggggaa 9480attaatgcac tgcatgacta ttcgaaaaaa ttcactagcc
ttacttagat gttagattaa 9540tagctagggg gtgcagataa ttttgaaagg
catgaaaaac attaatttgt acattgcaag 9600cttttgatga caagctttgc
aattgttcac actaccttat gccatttata aatagagtga 9660ttggcatatg
aaggaaatca tgagagtcga agcgaaaaac aaagcttgag agtgtaggaa
9720aaatacagtt tttttggtaa aaatacagta tttgaatagg agcgaaaaat
atcctttcaa 9780aatgatcctt ttcttttttt ttttttttct tgttgttctt
ggtcagttat tcaaaggaaa 9840agggattgaa ataaaaactt gcatgtggga
tcgtacgtcg agtcgacctg ca 9892496021DNAArtificial Sequenceplasmid
pKR271 49ggccgcgaat tcaatcacta gtgaattcgc ggccgcatga gccgtaaagg
ttcaatacaa 60cgagtgcttg ttttcttagg gacaagcatt gtacttatgt atgattctgt
gtaaccatga 120gtcttccacg ttgtactaat gtgaagggca aaaataaaac
acagaacaag ttcgtttttc 180tcaaataatg tgaaggtaga aaatggaacc
atgcctcctc tcttgcatgt gatttaaaat 240attagcagat ggtaccgtac
gtgggcggat cccccgggct gcaggaattc actggccgtc 300gttttacaac
gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
360catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
cccttcccaa 420cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
attttctcct tacgcatctg 480tgcggtattt cacaccgcat atggtgcact
ctcagtacaa tctgctctga tgccgcatag 540ttaagccagc cccgacaccc
gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 600ccggcatccg
cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
660tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
atttttatag 720gttaatgtca tgataataat ggtttcttag acgtcaggtg
gcacttttcg gggaaatgtg 780cgcggaaccc ctatttgttt atttttctaa
atacattcaa atatgtatcc gctcatgaga 840caataaccct gataaatgct
tcaataatat tgaaaaagga agagtatgag tattcaacat 900ttccgtgtcg
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
960gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
gggttacatc 1020gaactggatc tcaacagcgg taagatcctt gagagttttc
gccccgaaga acgttttcca 1080atgatgagca cttttaaagt tctgctatgt
ggcgcggtat tatcccgtat tgacgccggg 1140caagagcaac tcggtcgccg
catacactat tctcagaatg acttggttga gtactcacca 1200gtcacagaaa
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
1260accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
accgaaggag 1320ctaaccgctt ttttgcacaa catgggggat catgtaactc
gccttgatcg ttgggaaccg 1380gagctgaatg aagccatacc aaacgacgag
cgtgacacca cgatgcctgt agcaatggca 1440acaacgttgc gcaaactatt
aactggcgaa ctacttactc tagcttcccg gcaacaatta 1500atagactgga
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
1560ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
tatcattgca 1620gcactggggc cagatggtaa gccctcccgt atcgtagtta
tctacacgac ggggagtcag 1680gcaactatgg atgaacgaaa tagacagatc
gctgagatag gtgcctcact gattaagcat 1740tggtaactgt cagaccaagt
ttactcatat atactttaga ttgatttaaa acttcatttt 1800taatttaaaa
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
1860cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
atcttcttga 1920gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
aaaaaccacc gctaccagcg 1980gtggtttgtt tgccggatca agagctacca
actctttttc cgaaggtaac tggcttcagc 2040agagcgcaga taccaaatac
tgtccttcta gtgtagccgt agttaggcca ccacttcaag 2100aactctgtag
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
2160agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
ggataaggcg 2220cagcggtcgg gctgaacggg gggttcgtgc acacagccca
gcttggagcg aacgacctac 2280accgaactga gatacctaca gcgtgagcta
tgagaaagcg ccacgcttcc cgaagggaga 2340aaggcggaca ggtatccggt
aagcggcagg gtcggaacag gagagcgcac gagggagctt 2400ccagggggaa
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
2460cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
cagcaacgcg 2520gcctttttac ggttcctggc cttttgctgg ccttttgctc
acatgttctt tcctgcgtta 2580tcccctgatt ctgtggataa ccgtattacc
gcctttgagt gagctgatac cgctcgccgc 2640agccgaacga ccgagcgcag
cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2700aaaccgcctc
tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
2760gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
tcattaggca 2820ccccaggctt tacactttat gcttccggct cgtatgttgt
gtggaattgt gagcggataa 2880caatttcaca caggaaacag ctatgaccat
gattacgcca agcttgcatg cctgcaggtc 2940gactcgacgt acgatcccac
atgcaagttt ttatttcaat cccttttcct ttgaataact 3000gaccaagaac
aacaagaaaa aaaaaaaaaa agaaaaggat cattttgaaa ggatattttt
3060cgctcctatt caaatactgt atttttacca aaaaaactgt atttttccta
cactctcaag 3120ctttgttttt cgcttcgact ctcatgattt ccttcatatg
ccaatcactc tatttataaa 3180tggcataagg tagtgtgaac aattgcaaag
cttgtcatca aaagcttgca atgtacaaat 3240taatgttttt catgcctttc
aaaattatct gcacccccta gctattaatc taacatctaa 3300gtaaggctag
tgaatttttt cgaatagtca tgcagtgcat taatttcccc gtgactattt
3360tggctttgac tccaacactg gccccgtaca tccgtccctc attacatgaa
aagaaatatt 3420gtttatattc ttaattaaaa atattgtccc ttctaaattt
tcatatagtt aattattata 3480ttactttttt ctctattcta ttagttctat
tttcaaatta ttatttatgc atatgtaaag 3540tacattatat ttttgctata
tacttaaata tttctaaatt attaaaaaaa gactgatatg 3600aaaaatttat
tctttttaaa gctatatcat tttatatata ctttttcttt tcttttcttt
3660cattttctat tcaatttaat aagaaataaa ttttgtaaat ttttatttat
caatttataa 3720aaatatttta ctttatatgt tttttcacat ttttgttaaa
caaatcatat cattatgatt 3780gaaagagagg aaattgacag tgagtaataa
gtgatgagaa aaaaatgtgt tatttcctaa 3840aaaaaaccta aacaaacatg
tatctactct ctatttcatc tatctctcat ttcatttttc 3900tctttatctc
tttctttatt tttttatcat atcatttcac attaattatt tttactctct
3960ttattttttc tctctatccc tctcttattt ccactcatat atacactcca
aaattggggc 4020atgcctttat cactactcta tctcctccac taaatcattt
aaatgaaact gaaaagcatt 4080ggcaagtctc ctcccctcct caagtgattt
ccaactcagc attggcatct aattgattca 4140gtatatctat tgcatgtgta
aaagtctttc cacaatacat aactattaat taatcttaaa 4200taaataaagg
ataaaatatt tttttttctt cataaaatta aaatatgtta ttttttgttt
4260agatgtatat tcgaataaat ctaaatatat gataatgatt ttttatattg
attaaacata 4320taatcaatat taaatatgat atttttttat ataggttgta
cacataattt tataaggata 4380aaaaatatga taaaaataaa ttttaaatat
ttttatattt acgagaaaaa aaaatatttt 4440agccataaat aaatgaccag
catattttac aaccttagta attcataaat tcctatatgt 4500atatttgaaa
ttaaaaacag ataatcgtta agggaaggaa tcctacgtca tctcttgcca
4560tttgtttttc atgcaaacag aaagggacga aaaaccacct caccatgaat
cactcttcac 4620accattttta ctagcaaaca agtctcaaca actgaagcca
gctctctttc cgtttctttt 4680tacaacactt tctttgaaat agtagtattt
ttttttcaca tgatttatta acgtgccaaa 4740agatgcttat tgaatagagt
gcacatttgt aatgtactac taattagaac atgaaaaagc 4800attgttctaa
cacgataatc ctgtgaaggc gttaactcca aagatccaat ttcactatat
4860aaattgtgac gaaagcaaaa tgaattcaca tagctgagag agaaaggaaa
ggttaactaa 4920gaagcaatac ttcagcggcc gcatgactga ggataagacg
aaggtcgagt tcccgacgct 4980cacggagctc aagcactcga tcccgaacgc
gtgctttgag tcgaacctcg gcctctcgct 5040ctactacacg gcccgcgcga
tcttcaacgc gtcggcctcg gcggcgctgc tctacgcggc 5100gcgctcgacg
ccgttcattg ccgataacgt tctgctccac gcgctcgttt gcgccaccta
5160catctacgtg cagggcgtca tcttctgggg cttcttcacg gtcggccacg
actgcggcca 5220ctcggccttc tcgcgctacc acagcgtcaa ctttatcatc
ggctgcatca tgcactctgc 5280gattttgacg ccgttcgaga gctggcgcgt
gacgcaccgc caccaccaca agaacacggg 5340caacattgat aaggacgaga
tcttttaccc gcaccggtcg gtcaaggacc tccaggacgt 5400gcgccaatgg
gtctacacgc tcggcggtgc gtggtttgtc tacttgaagg tcgggtatgc
5460cccgcgcacg atgagccact ttgacccgtg ggacccgctc ctccttcgcc
gcgcgtcggc 5520cgtcatcgtg tcgctcggcg tctgggccgc cttcttcgcc
gcgtacgcgt acctcacata 5580ctcgctcggc tttgccgtca tgggcctcta
ctactatgcg ccgctctttg tctttgcttc 5640gttcctcgtc attacgacct
tcttgcacca caacgacgaa gcgacgccgt ggtacggcga 5700ctcggagtgg
acgtacgtca agggcaacct ctcgagcgtc gaccgctcgt acggcgcgtt
5760cgtggacaac ctgagccacc acattggcac gcaccaggtc caccacttgt
tcccgatcat 5820tccgcactac aagctcaacg aagccaccaa gcactttgcg
gccgcgtacc cgcacctcgt 5880gcgcaggaac gacgagccca tcatcacggc
cttcttcaag accgcgcacc tctttgtcaa 5940ctacggcgct gtgcccgaga
cggcgcagat cttcacgctc aaagagtcgg ccgcggccgc 6000caaggccaag
tcggactaag c 6021506524DNAArtificial Sequenceplasmid pKR226
50gtacgtctag aggatccgtc gacggcgcgc ccgatcatcc ggatatagtt cctcctttca
60gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag
120cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga
tcgatccaag 180ctgtacctca ctattccttt gccctcggac gagtgctggg
gcgtcggttt ccactatcgg 240cgagtacttc tacacagcca tcggtccaga
cggccgcgct tctgcgggcg atttgtgtac 300gcccgacagt cccggctccg
gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg 360catcatcgaa
attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
420tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg
aagtagcgcg 480tctgctgctc catacaagcc aaccacggcc tccagaagaa
gatgttggcg acctcgtatt 540gggaatcccc gaacatcgcc tcgctccagt
caatgaccgc tgttatgcgg ccattgtccg 600tcaggacatt gttggagccg
aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct 660cggcccaaag
catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
720tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa
tcacgccatg 780tagtgtattg accgattcct tgcggtccga atgggccgaa
cccgctcgtc tggctaagat 840cggccgcagc gatcgcatcc atagcctccg
cgaccggctg cagaacagcg ggcagttcgg 900tttcaggcag gtcttgcaac
gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc 960tctcgctgaa
ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
1020gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc
cgcaggacat 1080atccacgccc tcctacatcg aagctgaaag cacgagattc
ttcgccctcc gagagctgca 1140tcaggtcgga gacgctgtcg aacttttcga
tcagaaactt ctcgacagac gtcgcggtga 1200gttcaggctt ttccatgggt
atatctcctt cttaaagtta
aacaaaatta tttctagagg 1260gaaaccgttg tggtctccct atagtgagtc
gtattaattt cgcgggatcg agatctgatc 1320aacctgcatt aatgaatcgg
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 1380tccgcttcct
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
1440gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
aggaaagaac 1500atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
aggccgcgtt gctggcgttt 1560ttccataggc tccgcccccc tgacgagcat
cacaaaaatc gacgctcaag tcagaggtgg 1620cgaaacccga caggactata
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 1680tctcctgttc
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
1740gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
cgttcgctcc 1800aagctgggct gtgtgcacga accccccgtt cagcccgacc
gctgcgcctt atccggtaac 1860tatcgtcttg agtccaaccc ggtaagacac
gacttatcgc cactggcagc agccactggt 1920aacaggatta gcagagcgag
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 1980aactacggct
acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
2040ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
tagcggtggt 2100ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
gatctcaaga agatcctttg 2160atcttttcta cggggtctga cgctcagtgg
aacgaaaact cacgttaagg gattttggtc 2220atgacattaa cctataaaaa
taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt 2280gatgacggtg
aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
2340gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
cgggtgtcgg 2400ggctggctta actatgcggc atcagagcag attgtactga
gagtgcacca tatggacata 2460ttgtcgttag aacgcggcta caattaatac
ataaccttat gtatcataca catacgattt 2520aggtgacact atagaacggc
gcgccaagct gggtctagaa ctagaaacgt gatgccactt 2580gttattgaag
tcgattacag catctattct gttttactat ttataacttt gccatttctg
2640acttttgaaa actatctctg gatttcggta tcgctttgtg aagatcgagc
aaaagagacg 2700ttttgtggac gcaatggtcc aaatccgttc tacatgaaca
aattggtcac aatttccact 2760aaaagtaaat aaatggcaag ttaaaaaagg
aatatgcatt ttactgattg cctaggtgag 2820ctccaagaga agttgaatct
acacgtctac caaccgctaa aaaaagaaaa acattgatat 2880gtaacctgat
tccattagct tttgacttct tcaacagatt ctctacttag atttctaaca
2940gaaatattat tactagcaca tcattttcag tctcactaca gcaaaaaatc
caacggcaca 3000atacagacaa caggagatat cagactacag agatagatag
atgctactgc atgtagtaag 3060ttaaataaaa ggaaaataaa atgtcttgct
accaaaacta ctacagacta tgatgctcac 3120cacaggccaa atcctgcaac
taggacagca ttatcttata tatattgtac aaaacaagca 3180tcaaggaaca
tttggtctag gcaatcagta cctcgttcta ccatcaccct cagttatcac
3240atccttgaag gatccattac tgggaatcat cggcaacaca tgctcctgat
ggggcacaat 3300gacatcaaga aggtaggggc caggggtgtc caacattctc
tgaattgccg ctctaagctc 3360ttccttcttc gtcactcgcg ctgccggtat
cccacaagca tcagcaaact tgagcatgtt 3420tgggaatatc tcgctctcgc
tagacggatc tccaagatag gtgtgagctc tattggactt 3480gtagaaccta
tcctccaact gaaccaccat acccaaatgc tgattgttca acaacaatat
3540cttaactggg agattctcca ctcttatagt ggccaactcc tgaacattca
tgatgaaact 3600accatcccca tcaatgtcaa ccacaacagc cccagggtta
gcaacagcag caccaatagc 3660cgcaggcaat ccaaaaccca tggctccaag
accccctgag gtcaaccact gcctcggtct 3720cttgtacttg taaaactgcg
cagcccacat ttgatgctgc ccaaccccag tactaacaat 3780agcatctcca
ttagtcaact catcaagaac ctcgatagca tgctgcggag aaatcgcgtc
3840ctggaatgtc ttgtaaccca atggaaactt gtgtttctgc acattaatct
cttctctcca 3900acctccaaga tcaaacttac cctccactcc tttctcctcc
aaaatcatat taattccctt 3960caaggccaac ttcaaatccg cgcaaaccga
cacgtgcgcc tgcttgttct tcccaatctc 4020ggcagaatca atatcaatgt
gaacaatctt agccctacta gcaaaagcct caagcttccc 4080agtaacacgg
tcatcaaacc ttaccccaaa ggcaagcaac aaatcactat tgtcaacagc
4140atagttagca taaacagtac catgcatacc cagcatctga agggaatatt
catcaccaat 4200aggaaaagtt ccaagaccca ttaaagtgct agcaacggga
ataccagtga gttcaacaaa 4260gcgcctcaat tcagcactgg aattcaaact
gccaccgccg acgtagagaa cgggcttttg 4320ggcctccatg atgagtctga
caatgtgttc caattgggcc tcggcggggg gcctgggcag 4380cctggcgagg
taaccgggga ggttaacggg ctcgtcccaa ttaggcacgg cgagttgctg
4440ctgaacgtct ttgggaatgt cgatgaggac cggaccgggg cggccggagg
tggcgacgaa 4500gaaagcctcg gcgacgacgc gggggatgtc gtcgacgtcg
aggatgaggt agttgtgctt 4560cgtgatggat ctgctcacct ccacgatcgg
ggtttcttgg aaggcgtcgg tgccgatcat 4620ccggcgggcg acctggccgg
tgatggcgac gactgggacg ctgtccatta aagcgtcggc 4680gaggccgctc
acgaggttgg tggcgccggg gccggaggtg gcaatgcaga cgccggggag
4740gccggaggaa cgcgcgtagc cttcggcggc gaagacgccg ccctgctcgt
ggcgcgggag 4800cacgttgcgg atggcggcgg agcgcgtgag cgcctggtgg
atctccatcg acgcaccgcc 4860ggggtacgcg aacaccgtcg tcacgccctg
cctctccagc gcctccacaa ggatgtccgc 4920gcccttgcga ggttcgccgg
aggcgaaccg tgacacgaag ggctccgtgg tcggcgcttc 4980cttggtgaag
ggcgccgccg tggggggttt ggagatggaa catttgattt tgagagcgtg
5040gttgggtttg gtgagggttt gatgagagag agggagggtg gatctagtaa
tgcgtttggg 5100gaaggtgggg tgtgaagagg aagaagagaa tcgggtggtt
ctggaagcgg tggccgccat 5160tgtgttgtgt ggcatggtta tacttcaaaa
actgcacaac aagcctagag ttagtaccta 5220aacagtaaat ttacaacaga
gagcaaagac acatgcaaaa atttcagcca taaaaaaagt 5280tataatagaa
tttaaagcaa aagtttcatt ttttaaacat atatacaaac aaactggatt
5340tgaaggaagg gattaattcc cctgctcaaa gtttgaattc ctattgtgac
ctatactcga 5400ataaaattga agcctaagga atgtatgaga aacaagaaaa
caaaacaaaa ctacagacaa 5460acaagtacaa ttacaaaatt cgctaaaatt
ctgtaatcac caaaccccat ctcagtcagc 5520acaaggccca aggtttattt
tgaaataaaa aaaaagtgat tttatttctc ataagctaaa 5580agaaagaaag
gcaattatga aatgatttcg actagatctg aaagtccaac gcgtattccg
5640cagatattaa agaaagagta gagtttcaca tggatcctag atggacccag
ttgaggaaaa 5700agcaaggcaa agcaaaccag aagtgcaaga tccgaaattg
aaccacggaa tctaggattt 5760ggtagaggga gaagaaaagt accttgagag
gtagaagaga agagaagagc agagagatat 5820atgaacgagt gtgtcttggt
ctcaactctg aagcgatacg agtttagagg ggagcattga 5880gttccaattt
atagggaaac cgggtggcag gggtgagtta atgacggaaa agcccctaag
5940taacgagatt ggattgtggg ttagattcaa ccgtttgcat ccgcggctta
gattggggaa 6000gtcagagtga atctcaaccg ttgactgagt tgaaaattga
atgtagcaac caattgagcc 6060aaccccagcc tttgcccttt gattttgatt
tgtttgttgc atacttttta tttgtcttct 6120ggttctgact ctctttctct
cgtttcaatg ccaggttgcc tactcccaca ccactcacaa 6180gaagattcta
ctgttagtat taaatatttt ttaatgtatt aaatgatgaa tgcttttgta
6240aacagaacaa gactatgtct aataagtgtc ttgcaacatt ttttaagaaa
ttaaaaaaaa 6300tatatttatt atcaaaatca aatgtatgaa aaatcatgaa
taatataatt ttatacattt 6360ttttaaaaaa tcttttaatt tcttaattaa
tatcttaaaa ataatgatta atatttaacc 6420caaaataatt agtatgattg
gtaaggaaga tatccatgtt atgtttggat gtgagtttga 6480tctagagcaa
agcttactag agtcgacctg caggtcgact cgac 65245132DNAArtificial
SequenceoCon-1 primer 51aatctagacc tgcaggatcc atgcccttca tt
325233DNAArtificial SequenceoCon-2 primer 52tttctagacc tgcaggttga
aacatccctg aag 33534480DNAArtificial Sequenceplasmid pKR179
53ctagacctgc aggatccatg cccttcattt gccgcttatt aattaatttg gtaacagtcc
60gtactaatca gttacttatc cttcccccat cataattaat cttggtagtc tcgaatgcca
120caacactgac tagtctcttg gatcataaga aaaagccaag gaacaaaaga
agacaaaaca 180caatgagagt atcctttgca tagcaatgtc taagttcata
aaattcaaac aaaaacgcaa 240tcacacacag tggacatcac ttatccacta
gctgatcagg atcgccgcgt caagaaaaaa 300aaactggacc ccaaaagcca
tgcacaacaa cacgtactca caaaggtgtc aatcgagcag 360cccaaaacat
tcaccaactc aacccatcat gagccctcac atttgttgtt tctaacccaa
420cctcaaactc gtattctctt ccgccacctc atttttgttt atttcaacac
ccgtcaaact 480gcatgccacc ccgtggccaa atgtccatgc atgttaacaa
gacctatgac tataaatagc 540tgcaatctcg gcccaggttt tcatcatcaa
gaaccagttc aatatcctag tacaccgtat 600taaagaattt aagatatact
gcggccgcaa gtatgaacta aaatgcatgt aggtgtaaga 660gctcatggag
agcatggaat attgtatccg accatgtaac agtataataa ctgagctcca
720tctcacttct tctatgaata aacaaaggat gttatgatat attaacactc
tatctatgca 780ccttattgtt ctatgataaa tttcctctta ttattataaa
tcatctgaat cgtgacggct 840tatggaatgc ttcaaatagt acaaaaacaa
atgtgtacta taagactttc taaacaattc 900taaccttagc attgtgaacg
agacataagt gttaagaaga cataacaatt ataatggaag 960aagtttgtct
ccatttatat attatatatt acccacttat gtattatatt aggatgttaa
1020ggagacataa caattataaa gagagaagtt tgtatccatt tatatattat
atactaccca 1080tttatatatt atacttatcc acttatttaa tgtctttata
aggtttgatc catgatattt 1140ctaatatttt agttgatatg tatatgaaag
ggtactattt gaactctctt actctgtata 1200aaggttggat catccttaaa
gtgggtctat ttaattttat tgcttcttac agataaaaaa 1260aaaattatga
gttggtttga taaaatattg aaggatttaa aataataata aataacatat
1320aatatatgta tataaattta ttataatata acatttatct ataaaaaagt
aaatattgtc 1380ataaatctat acaatcgttt agccttgctg gacgaatctc
aattatttaa acgagagtaa 1440acatatttga ctttttggtt atttaacaaa
ttattattta acactatatg aaattttttt 1500ttttatcagc aaagaataaa
attaaattaa gaaggacaat ggtgtcccaa tccttataca 1560accaacttcc
acaagaaagt caagtcagag acaacaaaaa aacaagcaaa ggaaattttt
1620taatttgagt tgtcttgttt gctgcataat ttatgcagta aaacactaca
cataaccctt 1680ttagcagtag agcaatggtt gaccgtgtgc ttagcttctt
ttattttatt tttttatcag 1740caaagaataa ataaaataaa atgagacact
tcagggatgt ttcaacctgc aggtctagag 1800gatccccggg taccgagctc
gaattcactg gccgtcgttt tacaacgtcg tgactgggaa 1860aaccctggcg
ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
1920aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
gaatggcgaa 1980tggcgcctga tgcggtattt tctccttacg catctgtgcg
gtatttcaca ccgcatatgg 2040tgcactctca gtacaatctg ctctgatgcc
gcatagttaa gccagccccg acacccgcca 2100acacccgctg acgcgccctg
acgggcttgt ctgctcccgg catccgctta cagacaagct 2160gtgaccgtct
ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
2220agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
aataatggtt 2280tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
gaacccctat ttgtttattt 2340ttctaaatac attcaaatat gtatccgctc
atgagacaat aaccctgata aatgcttcaa 2400taatattgaa aaaggaagag
tatgagtatt caacatttcc gtgtcgccct tattcccttt 2460tttgcggcat
tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
2520gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
cagcggtaag 2580atccttgaga gttttcgccc cgaagaacgt tttccaatga
tgagcacttt taaagttctg 2640ctatgtggcg cggtattatc ccgtattgac
gccgggcaag agcaactcgg tcgccgcata 2700cactattctc agaatgactt
ggttgagtac tcaccagtca cagaaaagca tcttacggat 2760ggcatgacag
taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
2820aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
gcacaacatg 2880ggggatcatg taactcgcct tgatcgttgg gaaccggagc
tgaatgaagc cataccaaac 2940gacgagcgtg acaccacgat gcctgtagca
atggcaacaa cgttgcgcaa actattaact 3000ggcgaactac ttactctagc
ttcccggcaa caattaatag actggatgga ggcggataaa 3060gttgcaggac
cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
3120ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
tggtaagccc 3180tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
ctatggatga acgaaataga 3240cagatcgctg agataggtgc ctcactgatt
aagcattggt aactgtcaga ccaagtttac 3300tcatatatac tttagattga
tttaaaactt catttttaat ttaaaaggat ctaggtgaag 3360atcctttttg
ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
3420tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
gcgcgtaatc 3480tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
tttgtttgcc ggatcaagag 3540ctaccaactc tttttccgaa ggtaactggc
ttcagcagag cgcagatacc aaatactgtc 3600cttctagtgt agccgtagtt
aggccaccac ttcaagaact ctgtagcacc gcctacatac 3660ctcgctctgc
taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
3720gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
aacggggggt 3780tcgtgcacac agcccagctt ggagcgaacg acctacaccg
aactgagata cctacagcgt 3840gagctatgag aaagcgccac gcttcccgaa
gggagaaagg cggacaggta tccggtaagc 3900ggcagggtcg gaacaggaga
gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 3960tatagtcctg
tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
4020ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
cctggccttt 4080tgctggcctt ttgctcacat gttctttcct gcgttatccc
ctgattctgt ggataaccgt 4140attaccgcct ttgagtgagc tgataccgct
cgccgcagcc gaacgaccga gcgcagcgag 4200tcagtgagcg aggaagcgga
agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 4260ccgattcatt
aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
4320aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
ctttatgctt 4380ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
ttcacacagg aaacagctat 4440gaccatgatt acgccaagct tgcatgcctg
caggtcgact 4480549088DNAArtificial Sequenceplasmid pKR578
54ggccgcgaca caagtgtgag agtactaaat aaatgctttg gttgtacgaa atcattacac
60taaataaaat aatcaaagct tatatatgcc ttccgctaag gccgaatgca aagaaattgg
120ttctttctcg ttatcttttg ccacttttac tagtacgtat taattactac
ttaatcatct 180ttgtttacgg ctcattatat ccggtctaga ggatccaagg
ccgcgaagtt aaaagcaatg 240ttgtcacttg tcgtactaac acatgatgtg
atagtttatg ctagctagct ataacataag 300ctgtctctga gtgtgttgta
tattaataaa gatcatcact ggtgaatggt gatcgtgtac 360gtaccctact
tagtaggcaa tggaagcact tagagtgtgc tttgtgcatg gccttgcctc
420tgttttgaga cttttgtaat gttttcgagt ttaaatcttt gcctttgcgt
acggatccgt 480cgacggcgcg cccgatcatc cggatatagt tcctcctttc
agcaaaaaac ccctcaagac 540ccgtttagag gccccaaggg gttatgctag
ttattgctca gcggtggcag cagccaactc 600agcttccttt cgggctttgt
tagcagccgg atcgatccaa gctgtacctc actattcctt 660tgccctcgga
cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
720atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
tcccggctcc 780ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
gcatcatcga aattgccgtc 840aaccaagctc tgatagagtt ggtcaagacc
aatgcggagc atatacgccc ggagccgcgg 900cgatcctgca agctccggat
gcctccgctc gaagtagcgc gtctgctgct ccatacaagc 960caaccacggc
ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
1020ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
tgttggagcc 1080gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
tcggcccaaa gcatcagctc 1140atcgagagcc tgcgcgacgg acgcactgac
ggtgtcgtcc atcacagttt gccagtgata 1200cacatgggga tcagcaatcg
cgcatatgaa atcacgccat gtagtgtatt gaccgattcc 1260ttgcggtccg
aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
1320catagcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
ggtcttgcaa 1380cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
ctctcgctga attccccaat 1440gtcaagcact tccggaatcg ggagcgcggc
cgatgcaaag tgccgataaa cataacgatc 1500tttgtagaaa ccatcggcgc
agctatttac ccgcaggaca tatccacgcc ctcctacatc 1560gaagctgaaa
gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
1620gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
tttccatggg 1680tatatctcct tcttaaagtt aaacaaaatt atttctagag
ggaaaccgtt gtggtctccc 1740tatagtgagt cgtattaatt tcgcgggatc
gagatcgatc caattccaat cccacaaaaa 1800tctgagctta acagcacagt
tgctcctctc agagcagaat cgggtattca acaccctcat 1860atcaactact
acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
1920acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc
cactattaca 1980gaggcaagag cagcagctga cgcgtacaca acaagtcagc
aaacagacag gttgaacttc 2040atccccaaag gagaagctca actcaagccc
aagagctttg ctaaggccct aacaagccca 2100ccaaagcaaa aagcccactg
gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc 2160ccaaaagaga
tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
2220ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga
taatgagaag 2280gttagcctct tcaatttcag aaagaatgct gacccacaga
tggttagaga ggcctacgca 2340gcaggtctca tcaagacgat ctacccgagt
aacaatctcc aggagatcaa ataccttccc 2400aagaaggtta aagatgcagt
caaaagattc aggactaatt gcatcaagaa cacagagaaa 2460gacatatttc
tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
2520aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac
tgaatctaag 2580gccatgcatg gagtctaaga ttcaaatcga ggatctaaca
gaactcgccg tgaagactgg 2640cgaacagttc atacagagtc ttttacgact
caatgacaag aagaaaatct tcgtcaacat 2700ggtggagcac gacactctgg
tctactccaa aaatgtcaaa gatacagtct cagaagacca 2760aagggctatt
gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
2820cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct
cctacaaatg 2880ccatcattgc gataaaggaa aggctatcat tcaagatgcc
tctgccgaca gtggtcccaa 2940agatggaccc ccacccacga ggagcatcgt
ggaaaaagaa gacgttccaa ccacgtcttc 3000aaagcaagtg gattgatgtg
acatctccac tgacgtaagg gatgacgcac aatcccacta 3060tccttcgcaa
gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
3120gagctcattt ctctattact tcagccataa caaaagaact cttttctctt
cttattaaac 3180catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
tttctgatcg aaaagttcga 3240cagcgtctcc gacctgatgc agctctcgga
gggcgaagaa tctcgtgctt tcagcttcga 3300tgtaggaggg cgtggatatg
tcctgcgggt aaatagctgc gccgatggtt tctacaaaga 3360tcgttatgtt
tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
3420tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
gtgtcacgtt 3480gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
ccggtcgcgg aggccatgga 3540tgcgatcgct gcggccgatc ttagccagac
gagcgggttc ggcccattcg gaccgcaagg 3600aatcggtcaa tacactacat
ggcgtgattt catatgcgcg attgctgatc cccatgtgta 3660tcactggcaa
actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
3720gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
cggatttcgg 3780ctccaacaat gtcctgacgg acaatggccg cataacagcg
gtcattgact ggagcgaggc 3840gatgttcggg gattcccaat acgaggtcgc
caacatcttc ttctggaggc cgtggttggc 3900ttgtatggag cagcagacgc
gctacttcga gcggaggcat ccggagcttg caggatcgcc 3960gcggctccgg
gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
4020cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
tccgatccgg 4080agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
gcggccgtct ggaccgatgg 4140ctgtgtagaa gtactcgccg atagtggaaa
ccgacgcccc agcactcgtc cgagggcaaa 4200ggaatagtga ggtacctaaa
gaaggagtgc gtcgaagcag atcgttcaaa catttggcaa 4260taaagtttct
taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
4320ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
tatgagatgg 4380gtttttatga ttagagtccc gcaattatac atttaatacg
cgatagaaaa caaaatatag 4440cgcgcaaact aggataaatt atcgcgcgcg
gtgtcatcta tgttactaga tcgatgtcga 4500atcgatcaac ctgcattaat
gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 4560gcgctcttcc
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
4620ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
ataacgcagg 4680aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
cgtaaaaagg ccgcgttgct 4740ggcgtttttc cataggctcc gcccccctga
cgagcatcac aaaaatcgac gctcaagtca 4800gaggtggcga aacccgacag
gactataaag ataccaggcg tttccccctg gaagctccct 4860cgtgcgctct
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
4920gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
tgtaggtcgt
4980tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
gcgccttatc 5040cggtaactat cgtcttgagt ccaacccggt aagacacgac
ttatcgccac tggcagcagc 5100cactggtaac aggattagca gagcgaggta
tgtaggcggt gctacagagt tcttgaagtg 5160gtggcctaac tacggctaca
ctagaaggac agtatttggt atctgcgctc tgctgaagcc 5220agttaccttc
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
5280cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
ctcaagaaga 5340tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
gaaaactcac gttaagggat 5400tttggtcatg acattaacct ataaaaatag
gcgtatcacg aggccctttc gtctcgcgcg 5460tttcggtgat gacggtgaaa
acctctgaca catgcagctc ccggagacgg tcacagcttg 5520tctgtaagcg
gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
5580gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
tgcaccatat 5640ggacatattg tcgttagaac gcggctacaa ttaatacata
accttatgta tcatacacat 5700acgatttagg tgacactata gaacggcgcg
ccaagcttgg atctcctgca ggatctggcc 5760ggccggatct cgtacgtcct
cgaagagaag ggttaataac acatttttta acatttttaa 5820cacaaatttt
agttatttaa aaatttatta aaaaatttaa aataagaaga ggaactcttt
5880aaataaatct aacttacaaa atttatgatt tttaataagt tttcaccaat
aaaaaatgtc 5940ataaaaatat gttaaaaagt atattatcaa tattctcttt
atgataaata aaaagaaaaa 6000aaaaataaaa gttaagtgaa aatgagattg
aagtgacttt aggtgtgtat aaatatatca 6060accccgccaa caatttattt
aatccaaata tattgaagta tattattcca tagcctttat 6120ttatttatat
atttattata taaaagcttt atttgttcta ggttgttcat gaaatatttt
6180tttggtttta tctccgttgt aagaaaatca tgtgctttgt gtcgccactc
actattgcag 6240ctttttcatg cattggtcag attgacggtt gattgtattt
ttgtttttta tggttttgtg 6300ttatgactta agtcttcatc tctttatctc
ttcatcaggt ttgatggtta cctaatatgg 6360tccatgggta catgcatggt
taaattaggt ggccaacttt gttgtgaacg atagaatttt 6420ttttatatta
agtaaactat ttttatatta tgaaataata ataaaaaaaa tattttatca
6480ttattaacaa aatcatatta gttaatttgt taactctata ataaaagaaa
tactgtaaca 6540ttcacattac atggtaacat ctttccaccc tttcatttgt
tttttgtttg atgacttttt 6600ttcttgttta aatttatttc ccttctttta
aatttggaat acattatcat catatataaa 6660ctaaaatact aaaaacagga
ttacacaaat gataaataat aacacaaata tttataaatc 6720tagctgcaat
atatttaaac tagctatatc gatattgtaa aataaaacta gctgcattga
6780tactgataaa aaaatatcat gtgctttctg gactgatgat gcagtatact
tttgacattg 6840cctttatttt atttttcaga aaagctttct tagttctggg
ttcttcatta tttgtttccc 6900atctccattg tgaattgaat catttgcttc
gtgtcacaaa tacaatttag ntaggtacat 6960gcattggtca gattcacggt
ttattatgtc atgacttaag ttcatggtag tacattacct 7020gccacgcatg
cattatattg gttagatttg ataggcaaat ttggttgtca acaatataaa
7080tataaataat gtttttatat tacgaaataa cagtgatcaa aacaaacagt
tttatcttta 7140ttaacaagat tttgtttttg tttgatgacg ttttttaatg
tttacgcttt cccccttctt 7200ttgaatttag aacactttat catcataaaa
tcaaatacta aaaaaattac atatttcata 7260aataataaca caaatatttt
taaaaaatct gaaataataa tgaacaatat tacatattat 7320cacgaaaatt
cattaataaa aatattatat aaataaaatg taatagtagt tatatgtagg
7380aaaaaagtac tgcacgcata atatatacaa aaagattaaa atgaactatt
ataaataata 7440acactaaatt aatggtgaat catatcaaaa taatgaaaaa
gtaaataaaa tttgtaatta 7500acttctatat gtattacaca cacaaataat
aaataatagt aaaaaaaatt atgataaata 7560tttaccatct cataagatat
ttaaaataat gataaaaata tagattattt tttatgcaac 7620tagctagcca
aaaagagaac acgggtatat ataaaaagag tacctttaaa ttctactgta
7680cttcctttat tcctgacgtt tttatatcaa gtggacatac gtgaagattt
taattatcag 7740tctaaatatt tcattagcac ttaatacttt tctgttttat
tcctatccta taagtagtcc 7800cgattctccc aacattgctt attcacacaa
ctaactaaga aagtcttcca tagcccccca 7860agcggccgca caatggcgac
tcgacagcga actgccacca ctgttgtggt cgaggacctt 7920cccaaggtca
ctcttgaggc caagtctgaa cctgtgttcc ccgatatcaa gaccatcaag
7980gatgccattc ccgcgcactg cttccagccc tcgctcgtca cctcattcta
ctacgtcttc 8040cgcgattttg ccatggtctc tgccctcgtc tgggctgctc
tcacctacat ccccagcatc 8100cccgaccaga ccctccgcgt cgcagcttgg
atggtctacg gcttcgtcca gggtctgttc 8160tgcaccggtg tctggattct
cggccatgag tgcggccacg gtgctttctc tctccacgga 8220aaggtcaaca
atgtgaccgg ctggttcctc cactcgttcc tcctcgtccc ctacttcagc
8280tggaagtact ctcaccaccg ccaccaccgc ttcaccggcc acatggatct
cgacatggct 8340ttcgtcccca agactgagcc caagccctcc aagtcgctca
tgattgctgg cattgacgtc 8400gccgagcttg ttgaggacac ccccgctgct
cagatggtca agctcatctt ccaccagctt 8460ttcggatggc aggcgtacct
cttcttcaac gctagctctg gcaagggcag caagcagtgg 8520gagcccaaga
ctggcctctc caagtggttc cgagtcagtc acttcgagcc taccagcgct
8580gtcttccgcc ccaacgaggc catcttcatc ctcatctccg atatcggtct
tgctctaatg 8640ggaactgctc tgtactttgc ttccaagcaa gttggtgttt
cgaccattct cttcctctac 8700cttgttccct acctgtgggt tcaccactgg
ctcgttgcca ttacctacct ccaccaccac 8760cacaccgagc tccctcacta
caccgctgag ggctggacct acgtcaaggg agctctcgcc 8820actgtcgacc
gtgagtttgg cttcatcgga aagcacctct tccacggtat cattgagaag
8880cacgttgttc accatctctt ccctaagatc cccttctaca aggctgacga
ggccaccgag 8940gccatcaagc ccgtcattgg cgaccactac tgccacgacg
accgaagctt cctgggccag 9000ctgtggacca tcttcggcac gctcaagtac
gtcgagcacg accctgcccg acccggtgcc 9060atgcgatgga acaaggacta ggctaggc
9088555705DNAArtificial Sequenceplasmid pKR582 55ggccgcacaa
tggcgactcg acagcgaact gccaccactg ttgtggtcga ggaccttccc 60aaggtcactc
ttgaggccaa gtctgaacct gtgttccccg atatcaagac catcaaggat
120gccattcccg cgcactgctt ccagccctcg ctcgtcacct cattctacta
cgtcttccgc 180gattttgcca tggtctctgc cctcgtctgg gctgctctca
cctacatccc cagcatcccc 240gaccagaccc tccgcgtcgc agcttggatg
gtctacggct tcgtccaggg tctgttctgc 300accggtgtct ggattctcgg
ccatgagtgc ggccacggtg ctttctctct ccacggaaag 360gtcaacaatg
tgaccggctg gttcctccac tcgttcctcc tcgtccccta cttcagctgg
420aagtactctc accaccgcca ccaccgcttc accggccaca tggatctcga
catggctttc 480gtccccaaga ctgagcccaa gccctccaag tcgctcatga
ttgctggcat tgacgtcgcc 540gagcttgttg aggacacccc cgctgctcag
atggtcaagc tcatcttcca ccagcttttc 600ggatggcagg cgtacctctt
cttcaacgct agctctggca agggcagcaa gcagtgggag 660cccaagactg
gcctctccaa gtggttccga gtcagtcact tcgagcctac cagcgctgtc
720ttccgcccca acgaggccat cttcatcctc atctccgata tcggtcttgc
tctaatggga 780actgctctgt actttgcttc caagcaagtt ggtgtttcga
ccattctctt cctctacctt 840gttccctacc tgtgggttca ccactggctc
gttgccatta cctacctcca ccaccaccac 900accgagctcc ctcactacac
cgctgagggc tggacctacg tcaagggagc tctcgccact 960gtcgaccgtg
agtttggctt catcggaaag cacctcttcc acggtatcat tgagaagcac
1020gttgttcacc atctcttccc taagatcccc ttctacaagg ctgacgaggc
caccgaggcc 1080atcaagcccg tcattggcga ccactactgc cacgacgacc
gaagcttcct gggccagctg 1140tggaccatct tcggcacgct caagtacgtc
gagcacgacc ctgcccgacc cggtgccatg 1200cgatggaaca aggactaggc
taggcggccg caagtatgaa ctaaaatgca tgtaggtgta 1260agagctcatg
gagagcatgg aatattgtat ccgaccatgt aacagtataa taactgagct
1320ccatctcact tcttctatga ataaacaaag gatgttatga tatattaaca
ctctatctat 1380gcaccttatt gttctatgat aaatttcctc ttattattat
aaatcatctg aatcgtgacg 1440gcttatggaa tgcttcaaat agtacaaaaa
caaatgtgta ctataagact ttctaaacaa 1500ttctaacctt agcattgtga
acgagacata agtgttaaga agacataaca attataatgg 1560aagaagtttg
tctccattta tatattatat attacccact tatgtattat attaggatgt
1620taaggagaca taacaattat aaagagagaa gtttgtatcc atttatatat
tatatactac 1680ccatttatat attatactta tccacttatt taatgtcttt
ataaggtttg atccatgata 1740tttctaatat tttagttgat atgtatatga
aagggtacta tttgaactct cttactctgt 1800ataaaggttg gatcatcctt
aaagtgggtc tatttaattt tattgcttct tacagataaa 1860aaaaaaatta
tgagttggtt tgataaaata ttgaaggatt taaaataata ataaataaca
1920tataatatat gtatataaat ttattataat ataacattta tctataaaaa
agtaaatatt 1980gtcataaatc tatacaatcg tttagccttg ctggacgaat
ctcaattatt taaacgagag 2040taaacatatt tgactttttg gttatttaac
aaattattat ttaacactat atgaaatttt 2100tttttttatc agcaaagaat
aaaattaaat taagaaggac aatggtgtcc caatccttat 2160acaaccaact
tccacaagaa agtcaagtca gagacaacaa aaaaacaagc aaaggaaatt
2220ttttaatttg agttgtcttg tttgctgcat aatttatgca gtaaaacact
acacataacc 2280cttttagcag tagagcaatg gttgaccgtg tgcttagctt
cttttatttt atttttttat 2340cagcaaagaa taaataaaat aaaatgagac
acttcaggga tgtttcaacc tgcaggtcta 2400gaggatcccc gggtaccgag
ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg 2460gaaaaccctg
gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
2520cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
cctgaatggc 2580gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
gcggtatttc acaccgcata 2640tggtgcactc tcagtacaat ctgctctgat
gccgcatagt taagccagcc ccgacacccg 2700ccaacacccg ctgacgcgcc
ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 2760gctgtgaccg
tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
2820gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
gataataatg 2880gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
gcggaacccc tatttgttta 2940tttttctaaa tacattcaaa tatgtatccg
ctcatgagac aataaccctg ataaatgctt 3000caataatatt gaaaaaggaa
gagtatgagt attcaacatt tccgtgtcgc ccttattccc 3060ttttttgcgg
cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
3120gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
caacagcggt 3180aagatccttg agagttttcg ccccgaagaa cgttttccaa
tgatgagcac ttttaaagtt 3240ctgctatgtg gcgcggtatt atcccgtatt
gacgccgggc aagagcaact cggtcgccgc 3300atacactatt ctcagaatga
cttggttgag tactcaccag tcacagaaaa gcatcttacg 3360gatggcatga
cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
3420gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
tttgcacaac 3480atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
agctgaatga agccatacca 3540aacgacgagc gtgacaccac gatgcctgta
gcaatggcaa caacgttgcg caaactatta 3600actggcgaac tacttactct
agcttcccgg caacaattaa tagactggat ggaggcggat 3660aaagttgcag
gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
3720tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
agatggtaag 3780ccctcccgta tcgtagttat ctacacgacg gggagtcagg
caactatgga tgaacgaaat 3840agacagatcg ctgagatagg tgcctcactg
attaagcatt ggtaactgtc agaccaagtt 3900tactcatata tactttagat
tgatttaaaa cttcattttt aatttaaaag gatctaggtg 3960aagatccttt
ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
4020gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
tctgcgcgta 4080atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
tggtttgttt gccggatcaa 4140gagctaccaa ctctttttcc gaaggtaact
ggcttcagca gagcgcagat accaaatact 4200gtccttctag tgtagccgta
gttaggccac cacttcaaga actctgtagc accgcctaca 4260tacctcgctc
tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
4320accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
ctgaacgggg 4380ggttcgtgca cacagcccag cttggagcga acgacctaca
ccgaactgag atacctacag 4440cgtgagctat gagaaagcgc cacgcttccc
gaagggagaa aggcggacag gtatccggta 4500agcggcaggg tcggaacagg
agagcgcacg agggagcttc cagggggaaa cgcctggtat 4560ctttatagtc
ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
4620tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
gttcctggcc 4680ttttgctggc cttttgctca catgttcttt cctgcgttat
cccctgattc tgtggataac 4740cgtattaccg cctttgagtg agctgatacc
gctcgccgca gccgaacgac cgagcgcagc 4800gagtcagtga gcgaggaagc
ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 4860tggccgattc
attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
4920cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
acactttatg 4980cttccggctc gtatgttgtg tggaattgtg agcggataac
aatttcacac aggaaacagc 5040tatgaccatg attacgccaa gcttgcatgc
ctgcaggtcg actctagacc tgcaggatcc 5100atgcccttca tttgccgctt
attaattaat ttggtaacag tccgtactaa tcagttactt 5160atccttcccc
catcataatt aatcttggta gtctcgaatg ccacaacact gactagtctc
5220ttggatcata agaaaaagcc aaggaacaaa agaagacaaa acacaatgag
agtatccttt 5280gcatagcaat gtctaagttc ataaaattca aacaaaaacg
caatcacaca cagtggacat 5340cacttatcca ctagctgatc aggatcgccg
cgtcaagaaa aaaaaactgg accccaaaag 5400ccatgcacaa caacacgtac
tcacaaaggt gtcaatcgag cagcccaaaa cattcaccaa 5460ctcaacccat
catgagccct cacatttgtt gtttctaacc caacctcaaa ctcgtattct
5520cttccgccac ctcatttttg tttatttcaa cacccgtcaa actgcatgcc
accccgtggc 5580caaatgtcca tgcatgttaa caagacctat gactataaat
agctgcaatc tcggcccagg 5640ttttcatcat caagaaccag ttcaatatcc
tagtacaccg tattaaagaa tttaagatat 5700actgc 57055612897DNAArtificial
Sequenceplasmid pKR983 56ggtcgactcg acgtacgtct agaggatccg
tcgacggcgc gcccgatcat ccggatatag 60ttcctccttt cagcaaaaaa cccctcaaga
cccgtttaga ggccccaagg ggttatgcta 120gttattgctc agcggtggca
gcagccaact cagcttcctt tcgggctttg ttagcagccg 180gatcgatcca
agctgtacct cactattcct ttgccctcgg acgagtgctg gggcgtcggt
240ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
cttctgcggg 300cgatttgtgt acgcccgaca gtcccggctc cggatcggac
gattgcgtcg catcgaccct 360gcgcccaagc tgcatcatcg aaattgccgt
caaccaagct ctgatagagt tggtcaagac 420caatgcggag catatacgcc
cggagccgcg gcgatcctgc aagctccgga tgcctccgct 480cgaagtagcg
cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
540cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
gctgttatgc 600ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
gtgcacgagg tgccggactt 660cggggcagtc ctcggcccaa agcatcagct
catcgagagc ctgcgcgacg gacgcactga 720cggtgtcgtc catcacagtt
tgccagtgat acacatgggg atcagcaatc gcgcatatga 780aatcacgcca
tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
840tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggc
tgcagaacag 900cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
ctgtgcacgg cgggagatgc 960aataggtcag gctctcgctg aattccccaa
tgtcaagcac ttccggaatc gggagcgcgg 1020ccgatgcaaa gtgccgataa
acataacgat ctttgtagaa accatcggcg cagctattta 1080cccgcaggac
atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
1140ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
ttctcgacag 1200acgtcgcggt gagttcaggc ttttccatgg gtatatctcc
ttcttaaagt taaacaaaat 1260tatttctaga gggaaaccgt tgtggtctcc
ctatagtgag tcgtattaat ttcgcgggat 1320cgagatctga tcaacctgca
ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 1380attgggcgct
cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
1440cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
aggggataac 1500gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
ggaaccgtaa aaaggccgcg 1560ttgctggcgt ttttccatag gctccgcccc
cctgacgagc atcacaaaaa tcgacgctca 1620agtcagaggt ggcgaaaccc
gacaggacta taaagatacc aggcgtttcc ccctggaagc 1680tccctcgtgc
gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
1740ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
ttcggtgtag 1800gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
ttcagcccga ccgctgcgcc 1860ttatccggta actatcgtct tgagtccaac
ccggtaagac acgacttatc gccactggca 1920gcagccactg gtaacaggat
tagcagagcg aggtatgtag gcggtgctac agagttcttg 1980aagtggtggc
ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
2040aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
aaccaccgct 2100ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
gcagaaaaaa aggatctcaa 2160gaagatcctt tgatcttttc tacggggtct
gacgctcagt ggaacgaaaa ctcacgttaa 2220gggattttgg tcatgacatt
aacctataaa aataggcgta tcacgaggcc ctttcgtctc 2280gcgcgtttcg
gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
2340gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
agcgggtgtt 2400ggcgggtgtc ggggctggct taactatgcg gcatcagagc
agattgtact gagagtgcac 2460catatggaca tattgtcgtt agaacgcggc
tacaattaat acataacctt atgtatcata 2520cacatacgat ttaggtgaca
ctatagaacg gcgcgccaag ctgggtctag aactagaaac 2580gtgatgccac
ttgttattga agtcgattac agcatctatt ctgttttact atttataact
2640ttgccatttc tgacttttga aaactatctc tggatttcgg tatcgctttg
tgaagatcga 2700gcaaaagaga cgttttgtgg acgcaatggt ccaaatccgt
tctacatgaa caaattggtc 2760acaatttcca ctaaaagtaa ataaatggca
agttaaaaaa ggaatatgca ttttactgat 2820tgcctaggtg agctccaaga
gaagttgaat ctacacgtct accaaccgct aaaaaaagaa 2880aaacattgat
atgtaacctg attccattag cttttgactt cttcaacaga ttctctactt
2940agatttctaa cagaaatatt attactagca catcattttc agtctcacta
cagcaaaaaa 3000tccaacggca caatacagac aacaggagat atcagactac
agagatagat agatgctact 3060gcatgtagta agttaaataa aaggaaaata
aaatgtcttg ctaccaaaac tactacagac 3120tatgatgctc accacaggcc
aaatcctgca actaggacag cattatctta tatatattgt 3180acaaaacaag
catcaaggaa catttggtct aggcaatcag tacctcgttc taccatcacc
3240ctcagttatc acatccttga aggatccatt actgggaatc atcggcaaca
catgctcctg 3300atggggcaca atgacatcaa gaaggtaggg gccaggggtg
tccaacattc tctgaattgc 3360cgctctaagc tcttccttct tcgtcactcg
cgctgccggt atcccacaag catcagcaaa 3420cttgagcatg tttgggaata
tctcgctctc gctagacgga tctccaagat aggtgtgagc 3480tctattggac
ttgtagaacc tatcctccaa ctgaaccacc atacccaaat gctgattgtt
3540caacaacaat atcttaactg ggagattctc cactcttata gtggccaact
cctgaacatt 3600catgatgaaa ctaccatccc catcaatgtc aaccacaaca
gccccagggt tagcaacagc 3660agcaccaata gccgcaggca atccaaaacc
catggctcca agaccccctg aggtcaacca 3720ctgcctcggt ctcttgtact
tgtaaaactg cgcagcccac atttgatgct gcccaacccc 3780agtactaaca
atagcatctc cattagtcaa ctcatcaaga acctcgatag catgctgcgg
3840agaaatcgcg tcctggaatg tcttgtaacc caatggaaac ttgtgtttct
gcacattaat 3900ctcttctctc caacctccaa gatcaaactt accctccact
cctttctcct ccaaaatcat 3960attaattccc ttcaaggcca acttcaaatc
cgcgcaaacc gacacgtgcg cctgcttgtt 4020cttcccaatc tcggcagaat
caatatcaat gtgaacaatc ttagccctac tagcaaaagc 4080ctcaagcttc
ccagtaacac ggtcatcaaa ccttacccca aaggcaagca acaaatcact
4140attgtcaaca gcatagttag cataaacagt accatgcata cccagcatct
gaagggaata 4200ttcatcacca ataggaaaag ttccaagacc cattaaagtg
ctagcaacgg gaataccagt 4260gagttcaaca aagcgcctca attcagcact
ggaattcaaa ctgccaccgc cgacgtagag 4320aacgggcttt tgggcctcca
tgatgagtct gacaatgtgt tccaattggg cctcggcggg 4380gggcctgggc
agcctggcga ggtaaccggg gaggttaacg ggctcgtccc aattaggcac
4440ggcgagttgc tgctgaacgt ctttgggaat gtcgatgagg accggaccgg
ggcggccgga 4500ggtggcgacg aagaaagcct cggcgacgac gcgggggatg
tcgtcgacgt cgaggatgag 4560gtagttgtgc ttcgtgatgg atctgctcac
ctccacgatc ggggtttctt ggaaggcgtc 4620ggtgccgatc atccggcggg
cgacctggcc ggtgatggcg acgactggga cgctgtccat 4680taaagcgtcg
gcgaggccgc tcacgaggtt ggtggcgccg gggccggagg tggcaatgca
4740gacgccgggg aggccggagg aacgcgcgta gccttcggcg gcgaagacgc
cgccctgctc 4800gtggcgcggg agcacgttgc ggatggcggc ggagcgcgtg
agcgcctggt ggatctccat 4860cgacgcaccg ccggggtacg cgaacaccgt
cgtcacgccc tgcctctcca gcgcctccac 4920aaggatgtcc gcgcccttgc
gaggttcgcc ggaggcgaac cgtgacacga agggctccgt 4980ggtcggcgct
tccttggtga agggcgccgc cgtggggggt ttggagatgg aacatttgat
5040tttgagagcg tggttgggtt tggtgagggt ttgatgagag
agagggaggg tggatctagt 5100aatgcgtttg gggaaggtgg ggtgtgaaga
ggaagaagag aatcgggtgg ttctggaagc 5160ggtggccgcc attgtgttgt
gtggcatggt tatacttcaa aaactgcaca acaagcctag 5220agttagtacc
taaacagtaa atttacaaca gagagcaaag acacatgcaa aaatttcagc
5280cataaaaaaa gttataatag aatttaaagc aaaagtttca ttttttaaac
atatatacaa 5340acaaactgga tttgaaggaa gggattaatt cccctgctca
aagtttgaat tcctattgtg 5400acctatactc gaataaaatt gaagcctaag
gaatgtatga gaaacaagaa aacaaaacaa 5460aactacagac aaacaagtac
aattacaaaa ttcgctaaaa ttctgtaatc accaaacccc 5520atctcagtca
gcacaaggcc caaggtttat tttgaaataa aaaaaaagtg attttatttc
5580tcataagcta aaagaaagaa aggcaattat gaaatgattt cgactagatc
tgaaagtcca 5640acgcgtattc cgcagatatt aaagaaagag tagagtttca
catggatcct agatggaccc 5700agttgaggaa aaagcaaggc aaagcaaacc
agaagtgcaa gatccgaaat tgaaccacgg 5760aatctaggat ttggtagagg
gagaagaaaa gtaccttgag aggtagaaga gaagagaaga 5820gcagagagat
atatgaacga gtgtgtcttg gtctcaactc tgaagcgata cgagtttaga
5880ggggagcatt gagttccaat ttatagggaa accgggtggc aggggtgagt
taatgacgga 5940aaagccccta agtaacgaga ttggattgtg ggttagattc
aaccgtttgc atccgcggct 6000tagattgggg aagtcagagt gaatctcaac
cgttgactga gttgaaaatt gaatgtagca 6060accaattgag ccaaccccag
cctttgccct ttgattttga tttgtttgtt gcatactttt 6120tatttgtctt
ctggttctga ctctctttct ctcgtttcaa tgccaggttg cctactccca
6180caccactcac aagaagattc tactgttagt attaaatatt ttttaatgta
ttaaatgatg 6240aatgcttttg taaacagaac aagactatgt ctaataagtg
tcttgcaaca ttttttaaga 6300aattaaaaaa aatatattta ttatcaaaat
caaatgtatg aaaaatcatg aataatataa 6360ttttatacat ttttttaaaa
aatcttttaa tttcttaatt aatatcttaa aaataatgat 6420taatatttaa
cccaaaataa ttagtatgat tggtaaggaa gatatccatg ttatgtttgg
6480atgtgagttt gatctagagc aaagcttact agagtcgacc tgcagcccgg
gggatccgcc 6540cacgtacggt accatctgct aatattttaa atcacatgca
agagaggagg catggttcca 6600ttttctacct tcacattatt tgagaaaaac
gaacttgttc tgtgttttat ttttgccctt 6660cacattagta caacgtggaa
gactcatggt tacacagaat catacataag tacaatgctt 6720gtccctaaga
aaacaagcac tcgttgtatt gaacctttac ggctcatgcg gccgcgaatt
6780cactagtgat tgaattcgcg gccgcttagt ccgacttggc cttggcggcc
gcggccgact 6840ctttgagcgt gaagatctgc gccgtctcgg gcacagcgcc
gtagttgaca aagaggtgcg 6900cggtcttgaa gaaggccgtg atgatgggct
cgtcgttcct gcgcacgagg tgcgggtacg 6960cggccgcaaa gtgcttggtg
gcttcgttga gcttgtagtg cggaatgatc gggaacaagt 7020ggtggacctg
gtgcgtgcca atgtggtggc tcaggttgtc cacgaacgcg ccgtacgagc
7080ggtcgacgct cgagaggttg cccttgacgt acgtccactc cgagtcgccg
taccacggcg 7140tcgcttcgtc gttgtggtgc aagaaggtcg taatgacgag
gaacgaagca aagacaaaga 7200gcggcgcata gtagtagagg cccatgacgg
caaagccgag cgagtatgtg aggtacgcgt 7260acgcggcgaa gaaggcggcc
cagacgccga gcgacacgat gacggccgac gcgcggcgaa 7320ggaggagcgg
gtcccacggg tcaaagtggc tcatcgtgcg cggggcatac ccgaccttca
7380agtagacaaa ccacgcaccg ccgagcgtgt agacccattg gcgcacgtcc
tggaggtcct 7440tgaccgaccg gtgcgggtaa aagatctcgt ccttatcaat
gttgcccgtg ttcttgtggt 7500ggtggcggtg cgtcacgcgc cagctctcga
acggcgtcaa aatcgcagag tgcatgatgc 7560agccgatgat aaagttgacg
ctgtggtagc gcgagaaggc cgagtggccg cagtcgtggc 7620cgaccgtgaa
gaagccccag aagatgacgc cctgcacgta gatgtaggtg gcgcaaacga
7680gcgcgtggag cagaacgtta tcggcaatga acggcgtcga gcgcgccgcg
tagagcagcg 7740ccgccgaggc cgacgcgttg aagatcgcgc gggccgtgta
gtagagcgag aggccgaggt 7800tcgactcaaa gcacgcgttc gggatcgagt
gcttgagctc cgtgagcgtc gggaactcga 7860ccttcgtctt atcctcagtc
atgcggccgc tgaagtattg cttcttagtt aacctttcct 7920ttctctctca
gctatgtgaa ttcattttgc tttcgtcaca atttatatag tgaaattgga
7980tctttggagt taacgccttc acaggattat cgtgttagaa caatgctttt
tcatgttcta 8040attagtagta cattacaaat gtgcactcta ttcaataagc
atcttttggc acgttaataa 8100atcatgtgaa aaaaaaatac tactatttca
aagaaagtgt tgtaaaaaga aacggaaaga 8160gagctggctt cagttgttga
gacttgtttg ctagtaaaaa tggtgtgaag agtgattcat 8220ggtgaggtgg
tttttcgtcc ctttctgttt gcatgaaaaa caaatggcaa gagatgacgt
8280aggattcctt cccttaacga ttatctgttt ttaatttcaa atatacatat
aggaatttat 8340gaattactaa ggttgtaaaa tatgctggtc atttatttat
ggctaaaata tttttttttc 8400tcgtaaatat aaaaatattt aaaatttatt
tttatcatat tttttatcct tataaaatta 8460tgtgtacaac ctatataaaa
aaatatcata tttaatattg attatatgtt taatcaatat 8520aaaaaatcat
tatcatatat ttagatttat tcgaatatac atctaaacaa aaaataacat
8580attttaattt tatgaagaaa aaaaaatatt ttatccttta tttatttaag
attaattaat 8640agttatgtat tgtggaaaga cttttacaca tgcaatagat
atactgaatc aattagatgc 8700caatgctgag ttggaaatca cttgaggagg
ggaggagact tgccaatgct tttcagtttc 8760atttaaatga tttagtggag
gagatagagt agtgataaag gcatgcccca attttggagt 8820gtatatatga
gtggaaataa gagagggata gagagaaaaa ataaagagag taaaaataat
8880taatgtgaaa tgatatgata aaaaaataaa gaaagagata aagagaaaaa
tgaaatgaga 8940gatagatgaa atagagagta gatacatgtt tgtttaggtt
ttttttagga aataacacat 9000ttttttctca tcacttatta ctcactgtca
atttcctctc tttcaatcat aatgatatga 9060tttgtttaac aaaaatgtga
aaaaacatat aaagtaaaat atttttataa attgataaat 9120aaaaatttac
aaaatttatt tcttattaaa ttgaatagaa aatgaaagaa aagaaaagaa
9180aaagtatata taaaatgata tagctttaaa aagaataaat ttttcatatc
agtctttttt 9240taataattta gaaatattta agtatatagc aaaaatataa
tgtactttac atatgcataa 9300ataataattt gaaaatagaa ctaatagaat
agagaaaaaa gtaatataat aattaactat 9360atgaaaattt agaagggaca
atatttttaa ttaagaatat aaacaatatt tcttttcatg 9420taatgaggga
cggatgtacg gggccagtgt tggagtcaaa gccaaaatag tcacggggaa
9480attaatgcac tgcatgacta ttcgaaaaaa ttcactagcc ttacttagat
gttagattaa 9540tagctagggg gtgcagataa ttttgaaagg catgaaaaac
attaatttgt acattgcaag 9600cttttgatga caagctttgc aattgttcac
actaccttat gccatttata aatagagtga 9660ttggcatatg aaggaaatca
tgagagtcga agcgaaaaac aaagcttgag agtgtaggaa 9720aaatacagtt
tttttggtaa aaatacagta tttgaatagg agcgaaaaat atcctttcaa
9780aatgatcctt ttcttttttt ttttttttct tgttgttctt ggtcagttat
tcaaaggaaa 9840agggattgaa ataaaaactt gcatgtggga tcgtacgtcg
agtcgacctg caggatccat 9900gcccttcatt tgccgcttat taattaattt
ggtaacagtc cgtactaatc agttacttat 9960ccttccccca tcataattaa
tcttggtagt ctcgaatgcc acaacactga ctagtctctt 10020ggatcataag
aaaaagccaa ggaacaaaag aagacaaaac acaatgagag tatcctttgc
10080atagcaatgt ctaagttcat aaaattcaaa caaaaacgca atcacacaca
gtggacatca 10140cttatccact agctgatcag gatcgccgcg tcaagaaaaa
aaaactggac cccaaaagcc 10200atgcacaaca acacgtactc acaaaggtgt
caatcgagca gcccaaaaca ttcaccaact 10260caacccatca tgagccctca
catttgttgt ttctaaccca acctcaaact cgtattctct 10320tccgccacct
catttttgtt tatttcaaca cccgtcaaac tgcatgccac cccgtggcca
10380aatgtccatg catgttaaca agacctatga ctataaatag ctgcaatctc
ggcccaggtt 10440ttcatcatca agaaccagtt caatatccta gtacaccgta
ttaaagaatt taagatatac 10500tgcggccgca caatggcgac tcgacagcga
actgccacca ctgttgtggt cgaggacctt 10560cccaaggtca ctcttgaggc
caagtctgaa cctgtgttcc ccgatatcaa gaccatcaag 10620gatgccattc
ccgcgcactg cttccagccc tcgctcgtca cctcattcta ctacgtcttc
10680cgcgattttg ccatggtctc tgccctcgtc tgggctgctc tcacctacat
ccccagcatc 10740cccgaccaga ccctccgcgt cgcagcttgg atggtctacg
gcttcgtcca gggtctgttc 10800tgcaccggtg tctggattct cggccatgag
tgcggccacg gtgctttctc tctccacgga 10860aaggtcaaca atgtgaccgg
ctggttcctc cactcgttcc tcctcgtccc ctacttcagc 10920tggaagtact
ctcaccaccg ccaccaccgc ttcaccggcc acatggatct cgacatggct
10980ttcgtcccca agactgagcc caagccctcc aagtcgctca tgattgctgg
cattgacgtc 11040gccgagcttg ttgaggacac ccccgctgct cagatggtca
agctcatctt ccaccagctt 11100ttcggatggc aggcgtacct cttcttcaac
gctagctctg gcaagggcag caagcagtgg 11160gagcccaaga ctggcctctc
caagtggttc cgagtcagtc acttcgagcc taccagcgct 11220gtcttccgcc
ccaacgaggc catcttcatc ctcatctccg atatcggtct tgctctaatg
11280ggaactgctc tgtactttgc ttccaagcaa gttggtgttt cgaccattct
cttcctctac 11340cttgttccct acctgtgggt tcaccactgg ctcgttgcca
ttacctacct ccaccaccac 11400cacaccgagc tccctcacta caccgctgag
ggctggacct acgtcaaggg agctctcgcc 11460actgtcgacc gtgagtttgg
cttcatcgga aagcacctct tccacggtat cattgagaag 11520cacgttgttc
accatctctt ccctaagatc cccttctaca aggctgacga ggccaccgag
11580gccatcaagc ccgtcattgg cgaccactac tgccacgacg accgaagctt
cctgggccag 11640ctgtggacca tcttcggcac gctcaagtac gtcgagcacg
accctgcccg acccggtgcc 11700atgcgatgga acaaggacta ggctaggcgg
ccgcaagtat gaactaaaat gcatgtaggt 11760gtaagagctc atggagagca
tggaatattg tatccgacca tgtaacagta taataactga 11820gctccatctc
acttcttcta tgaataaaca aaggatgtta tgatatatta acactctatc
11880tatgcacctt attgttctat gataaatttc ctcttattat tataaatcat
ctgaatcgtg 11940acggcttatg gaatgcttca aatagtacaa aaacaaatgt
gtactataag actttctaaa 12000caattctaac cttagcattg tgaacgagac
ataagtgtta agaagacata acaattataa 12060tggaagaagt ttgtctccat
ttatatatta tatattaccc acttatgtat tatattagga 12120tgttaaggag
acataacaat tataaagaga gaagtttgta tccatttata tattatatac
12180tacccattta tatattatac ttatccactt atttaatgtc tttataaggt
ttgatccatg 12240atatttctaa tattttagtt gatatgtata tgaaagggta
ctatttgaac tctcttactc 12300tgtataaagg ttggatcatc cttaaagtgg
gtctatttaa ttttattgct tcttacagat 12360aaaaaaaaaa ttatgagttg
gtttgataaa atattgaagg atttaaaata ataataaata 12420acatataata
tatgtatata aatttattat aatataacat ttatctataa aaaagtaaat
12480attgtcataa atctatacaa tcgtttagcc ttgctggacg aatctcaatt
atttaaacga 12540gagtaaacat atttgacttt ttggttattt aacaaattat
tatttaacac tatatgaaat 12600tttttttttt atcagcaaag aataaaatta
aattaagaag gacaatggtg tcccaatcct 12660tatacaacca acttccacaa
gaaagtcaag tcagagacaa caaaaaaaca agcaaaggaa 12720attttttaat
ttgagttgtc ttgtttgctg cataatttat gcagtaaaac actacacata
12780acccttttag cagtagagca atggttgacc gtgtgcttag cttcttttat
tttatttttt 12840tatcagcaaa gaataaataa aataaaatga gacacttcag
ggatgtttca acctgca 12897571272DNAArtificial Sequencesynthetic
delta-8 desaturase (codon-optimized for Yarrowia lipolytica)
57atgggcaagg gtggagacgg tggagcacag gctgtgtctg gcaccgatgc ctccctcgct
60gaggtctcct ctgtggacag caagtccgtc cacgtggttc tgtacggcaa gcgagtggat
120gtcaccaagt tccagaaggc tcaccctgga ggttcgaagg tgttccgaat
ctttcaggag 180cgagacgcca cagaacagtt cgagtcctac cactctccca
aggccatcaa gatgatggaa 240ggtatgctca aaaagtcgga ggatgctccc
gcttccgtgc ctcttccctc tcgatccact 300atgggcaccg agttcaagga
gatgatcgaa cgacacaaga gagccggtct ctacgaccct 360tgtcccttgg
acgagctgtt caagctcacc attgtccttg ctcctatctt tgtgggagcc
420tatctcgttc gatccggtgt ctctcctctt gctggagccc tgtcgatggg
cttcggattc 480tacctcgacg gctggcttgc tcacgactac ctgcatcacg
cagtgttcaa gggctccgtc 540aacacactcg tcaaggccaa caacgctatg
ggatacgcct tgggcttcct ccagggttac 600gacgttgctt ggtggcgagc
cagacacaac actcatcacg tgtgcaccaa cgaggacggc 660tccgatcccg
acatcaagac ggctcctctg ctcatttacg tgcgagagaa tccctccatt
720gccaagcggc tcaacttctt tcagcgatgg caacagtact actatgttcc
tactatggcc 780attctggatc tctactggcg actggagtct atcgcatacg
tcgctgtgcg actgcccaag 840atgtggatgc aggctgccgc tcttgccgct
cactacgcac tcctgtgttg ggtcttcgct 900gcccatctca acctgattcc
tctcatgatg gttgcacgag gtttcgcgac cggaatcgtg 960gtctttgcca
cccactatgg cgaggacatt ctcgaccgag agcacgtcga aggcatgact
1020ctggtcgagc agaccgccaa gacctcccga aacatcactg gtggatggct
tgtcaacgtg 1080ctcaccggct tcatttctct gcagaccgag catcatctgt
ttcccatgat gcctactgga 1140aacctcatga ccattcaacc cgaggttcga
gactttttca aaaagcacgg tctcgagtac 1200cgagaaggaa acctgtttca
gtgcgtgcat cagaacatca aggctctcgc cttcgagcac 1260ctgcttcact aa
1272583992DNAArtificial Sequenceplasmid pPiD8S 58tcgcgcgttt
cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60cagcttgtct
gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
120ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
ctgagagtgc 180accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
aaaataccgc atcaggcgcc 240attcgccatt caggctgcgc aactgttggg
aagggcgatc ggtgcgggcc tcttcgctat 300tacgccagct ggcgaaaggg
ggatgtgctg caaggcgatt aagttgggta acgccagggt 360tttcccagtc
acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
420tgcatctaga tccatgggca agggtggaga cggtggagca caggctgtgt
ctggcaccga 480tgcctccctc gctgaggtct cctctgtgga cagcaagtcc
gtccacgtgg ttctgtacgg 540caagcgagtg gatgtcacca agttccagaa
ggctcaccct ggaggttcga aggtgttccg 600aatctttcag gagcgagacg
ccacagaaca gttcgagtcc taccactctc ccaaggccat 660caagatgatg
gaaggtatgc tcaaaaagtc ggaggatgct cccgcttccg tgcctcttcc
720ctctcgatcc actatgggca ccgagttcaa ggagatgatc gaacgacaca
agagagccgg 780tctctacgac ccttgtccct tggacgagct gttcaagctc
accattgtcc ttgctcctat 840ctttgtggga gcctatctcg ttcgatccgg
tgtctctcct cttgctggag ccctgtcgat 900gggcttcgga ttctacctcg
acggctggct tgctcacgac tacctgcatc acgcagtgtt 960caagggctcc
gtcaacacac tcgtcaaggc caacaacgct atgggatacg ccttgggctt
1020cctccagggt tacgacgttg cttggtggcg agccagacac aacactcatc
acgtgtgcac 1080caacgaggac ggctccgatc ccgacatcaa gacggctcct
ctgctcattt acgtgcgaga 1140gaatccctcc attgccaagc ggctcaactt
ctttcagcga tggcaacagt actactatgt 1200tcctactatg gccattctgg
atctctactg gcgactggag tctatcgcat acgtcgctgt 1260gcgactgccc
aagatgtgga tgcaggctgc cgctcttgcc gctcactacg cactcctgtg
1320ttgggtcttc gctgcccatc tcaacctgat tcctctcatg atggttgcac
gaggtttcgc 1380gaccggaatc gtggtctttg ccacccacta tggcgaggac
attctcgacc gagagcacgt 1440cgaaggcatg actctggtcg agcagaccgc
caagacctcc cgaaacatca ctggtggatg 1500gcttgtcaac gtgctcaccg
gcttcatttc tctgcagacc gagcatcatc tgtttcccat 1560gatgcctact
ggaaacctca tgaccattca acccgaggtt cgagactttt tcaaaaagca
1620cggtctcgag taccgagaag gaaacctgtt tcagtgcgtg catcagaaca
tcaaggctct 1680cgccttcgag cacctgcttc actaagcggc cgcatcggat
cccgggcccg tcgactgcag 1740aggcctgcat gcaagcttgg cgtaatcatg
gtcatagctg tttcctgtgt gaaattgtta 1800tccgctcaca attccacaca
acatacgagc cggaagcata aagtgtaaag cctggggtgc 1860ctaatgagtg
agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
1920aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
gcggtttgcg 1980tattgggcgc tcttccgctt cctcgctcac tgactcgctg
cgctcggtcg ttcggctgcg 2040gcgagcggta tcagctcact caaaggcggt
aatacggtta tccacagaat caggggataa 2100cgcaggaaag aacatgtgag
caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2160gttgctggcg
tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2220aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
cccctggaag 2280ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
ggatacctgt ccgcctttct 2340cccttcggga agcgtggcgc tttctcatag
ctcacgctgt aggtatctca gttcggtgta 2400ggtcgttcgc tccaagctgg
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 2460cttatccggt
aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2520agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
cagagttctt 2580gaagtggtgg cctaactacg gctacactag aagaacagta
tttggtatct gcgctctgct 2640gaagccagtt accttcggaa aaagagttgg
tagctcttga tccggcaaac aaaccaccgc 2700tggtagcggt ggtttttttg
tttgcaagca gcagattacg cgcagaaaaa aaggatctca 2760agaagatcct
ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
2820agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
taaattaaaa 2880atgaagtttt aaatcaatct aaagtatata tgagtaaact
tggtctgaca gttaccaatg 2940cttaatcagt gaggcaccta tctcagcgat
ctgtctattt cgttcatcca tagttgcctg 3000actccccgtc gtgtagataa
ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3060aatgataccg
cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
3120cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
agtctattaa 3180ttgttgccgg gaagctagag taagtagttc gccagttaat
agtttgcgca acgttgttgc 3240cattgctaca ggcatcgtgg tgtcacgctc
gtcgtttggt atggcttcat tcagctccgg 3300ttcccaacga tcaaggcgag
ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 3360cttcggtcct
ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
3420ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
ctgtgactgg 3480tgagtactca accaagtcat tctgagaata gtgtatgcgg
cgaccgagtt gctcttgccc 3540ggcgtcaata cgggataata ccgcgccaca
tagcagaact ttaaaagtgc tcatcattgg 3600aaaacgttct tcggggcgaa
aactctcaag gatcttaccg ctgttgagat ccagttcgat 3660gtaacccact
cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
3720gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
cacggaaatg 3780ttgaatactc atactcttcc tttttcaata ttattgaagc
atttatcagg gttattgtct 3840catgagcgga tacatatttg aatgtattta
gaaaaataaa caaatagggg ttccgcgcac 3900atttccccga aaagtgccac
ctgacgtcta agaaaccatt attatcatga cattaaccta 3960taaaaatagg
cgtatcacga ggccctttcg tc 399259792DNAIsochrysis galbana
59atggccctcg caaacgacgc gggagagcgc atctgggcgg ctgtgaccga cccggaaatc
60ctcattggca ccttctcgta cttgctactc aaaccgctgc tccgcaattc cgggctggtg
120gatgagaaga agggcgcata caggacgtcc atgatctggt acaacgttct
gctggcgctc 180ttctctgcgc tgagcttcta cgtgacggcg accgccctcg
gctgggacta tggtacgggc 240gcgtggctgc gcaggcaaac cggcgacaca
ccgcagccgc tcttccagtg cccgtccccg 300gtttgggact cgaagctctt
cacatggacc gccaaggcat tctattactc caagtacgtg 360gagtacctcg
acacggcctg gctggtgctc aagggcaaga gggtctcctt tctccaggcc
420ttccaccact ttggcgcgcc gtgggatgtg tacctcggca ttcggctgca
caacgagggc 480gtatggatct tcatgttttt caactcgttc attcacacca
tcatgtacac ctactacggc 540ctcaccgccg ccgggtataa gttcaaggcc
aagccgctca tcaccgcgat gcagatctgc 600cagttcgtgg gcggcttcct
gttggtctgg gactacatca acgtcccctg cttcaactcg 660gacaaaggga
agttgttcag ctgggctttc aactatgcat acgtcggctc ggtcttcttg
720ctcttctgcc actttttcta ccaggacaac ttggcaacga agaaatcggc
caaggcgggc 780aagcagctct ag 79260757DNAEuglena
gracilismisc_feature(677)..(677)n is a, c, g, or t 60ttttttttcg
aacacttaat ggaggtggtg aatgaaatag tctcaattgg gcaggaagtt 60ttacccaaag
ttgattatgc ccaactctgg agtgatgcca gtcactgtga ggtgctttac
120ttgtccatcg catttgtcat cttgaagttc actcttggcc cccttggtcc
aaaaggtcag 180tctcgtatga agtttgtttt caccaattac aaccttctca
tgtccattta ttcgttggga 240tcattcctct caatggcata tgccatgtac
accatcggtg ttatgtctga caactgcgag 300aaggcttttg acaacaacgt
cttcaggatc accacgcagt tgttctattt gagcaagttc 360ctggagtata
ttgactcctt ctatttgcca ctgatgggca agcctctgac ctggttgcaa
420ttcttccatc atttgggggc accgatggat atgtggctgt tctataatta
ccgaaatgaa 480gctgtttgga tttttgtgct gttgaatggt ttcatccact
ggatcatgta cggttattat 540tggaccagat tgatcaagct gaagttcccc
atgccaaaat ccctgattac atcaatgcag 600atcattcaat tcaatgttgg
tttctacatt gtctggaagt acaggaacat tccctgttat 660cgccaagatg
ggatgangat gtttggctgg ttcttcaatt acttttatgt tggcacagtc
720ttgtgtttgt tcttgaattt ctatgtgcaa acgtata 75761774DNAEuglena
gracilismisc_feature(34)..(34)n is a, c,
g, or t 61tcaggatcac cacgcagttg ttctatttga gcangttcct ggagtatatt
gactccttct 60atttgccant gatgggcaag cntctgacct ggttgcaatt cttccatcat
tngggggcac 120cgatggatat gtggctgttc tataattacc gaaatgaagc
tgtttggatt tttgtgctgt 180tgaatggttt catccactgg atcatgtacg
gttattannn gaccagattg atcaagctga 240agttccccat gccaaaatcc
ctgattacat caatgcagat cattcaattc aatgttggtt 300tctacattgt
ctggaagtac aggaacattc cctgttatcg ccaagatggg atgaggatgt
360ttggctggtt cttcaattac ttttatgttg gcacagtctt gtgtttgttc
ttgaatttct 420atgtgcaaac gtatatcgtc aggaagcaca agggagccaa
aaagattcag tgatatttcc 480tcctctgcgg tggcctcttt tgacctcccc
ttgacaccta taatgtggag gtgtcgggct 540ctctccgtct caccagcact
tgactctgca ggtgctcact tttatttttt acccatcttt 600gcttgttgac
cattcacctc tcccacttcc acatagtcca ttctaactgt tgcagactgc
660ggtccatttt ttccagagct cccaatgacc atacgcgaca ccttgtnnnc
ncccagccca 720ttgtgcacaa ttcatagtgg catcgttttg ccttgatacg
tgtgcatcca gcgg 774621201DNAEuglena
gracilismisc_feature(1134)..(1136)n is a, c, g, or t 62gacatggcaa
ctatgatttt attttgactg atagtgacct gttcgttgca acaaattgat 60gagcaatgct
tttttataat gccaactttg tacaaaaaag ttggattttt tttcgaacac
120ttaatggagg tggtgaatga aatagtctca attgggcagg aagttttacc
caaagttgat 180tatgcccaac tctggagtga tgccagtcac tgtgaggtgc
tttacttgtc catcgcattt 240gtcatcttga agttcactct tggccccctt
ggtccaaaag gtcagtctcg tatgaagttt 300gttttcacca attacaacct
tctcatgtcc atttattcgt tgggatcatt cctctcaatg 360gcatatgcca
tgtacaccat cggtgttatg tctgacaact gcgagaaggc ttttgacaac
420aacgtcttca ggatcaccac gcagttgttc tatttgagca agttcctgga
gtatattgac 480tccttctatt tgccactgat gggcaagcct ctgacctggt
tgcaattctt ccatcatttg 540ggggcaccga tggatatgtg gctgttctat
aattaccgaa atgaagctgt ttggattttt 600gtgctgttga atggtttcat
ccactggatc atgtacggtt attattggac cagattgatc 660aagctgaagt
tccccatgcc aaaatccctg attacatcaa tgcagatcat tcaattcaat
720gttggtttct acattgtctg gaagtacagg aacattccct gttatcgcca
agatgggatg 780aggatgtttg gctggttctt caattacttt tatgttggca
cagtcttgtg tttgttcttg 840aatttctatg tgcaaacgta tatcgtcagg
aagcacaagg gagccaaaaa gattcagtga 900tatttcctcc tctgcggtgg
cctcttttga cctccccttg acacctataa tgtggaggtg 960tcgggctctc
tccgtctcac cagcacttga ctctgcaggt gctcactttt attttttacc
1020catctttgct tgttgaccat tcacctctcc cacttccaca tagtccattc
taactgttgc 1080agactgcggt ccattttttc cagagctccc aatgaccata
cgcgacacct tgtnnncncc 1140cagcccattg tgcacaattc atagtggcat
cgttttgcct tgatacgtgt gcatccagcg 1200g 12016318DNAArtificial
SequenceM13F universal primer 63tgtaaaacga cggccagt
1864258PRTEuglena gracilis 64Met Glu Val Val Asn Glu Ile Val Ser
Ile Gly Gln Glu Val Leu Pro1 5 10 15Lys Val Asp Tyr Ala Gln Leu Trp
Ser Asp Ala Ser His Cys Glu Val 20 25 30Leu Tyr Leu Ser Ile Ala Phe
Val Ile Leu Lys Phe Thr Leu Gly Pro 35 40 45Leu Gly Pro Lys Gly Gln
Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 50 55 60Asn Leu Leu Met Ser
Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala65 70 75 80Tyr Ala Met
Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 85 90 95Phe Asp
Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 100 105
110Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys
115 120 125Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro
Met Asp 130 135 140Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val
Trp Ile Phe Val145 150 155 160Leu Leu Asn Gly Phe Ile His Trp Ile
Met Tyr Gly Tyr Tyr Trp Thr 165 170 175Arg Leu Ile Lys Leu Lys Phe
Pro Met Pro Lys Ser Leu Ile Thr Ser 180 185 190Met Gln Ile Ile Gln
Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 195 200 205Arg Asn Ile
Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 210 215 220Phe
Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn225 230
235 240Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys
Lys 245 250 255Ile Gln65263PRTIsochrysis galbana 65Met Ala Leu Ala
Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr1 5 10 15Asp Pro Glu
Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro 20 25 30Leu Leu
Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg 35 40 45Thr
Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu 50 55
60Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly65
70 75 80Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe
Gln 85 90 95Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr
Ala Lys 100 105 110Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp
Thr Ala Trp Leu 115 120 125Val Leu Lys Gly Lys Arg Val Ser Phe Leu
Gln Ala Phe His His Phe 130 135 140Gly Ala Pro Trp Asp Val Tyr Leu
Gly Ile Arg Leu His Asn Glu Gly145 150 155 160Val Trp Ile Phe Met
Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr 165 170 175Thr Tyr Tyr
Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro 180 185 190Leu
Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu 195 200
205Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys
210 215 220Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val
Phe Leu225 230 235 240Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu
Ala Thr Lys Lys Ser 245 250 255Ala Lys Ala Gly Lys Gln Leu
26066777DNAArtificial Sequencesynthetic (codon-optimized) delta-9
elongase derived from Isochrysis galbana codon-optimized for
expression in Yarrowia lipolytica 66atggaggtcg tgaacgaaat
cgtctccatt ggccaggagg ttcttcccaa ggtcgactat 60gctcagctct ggtctgatgc
ctcgcactgc gaggtgctgt acctctccat cgccttcgtc 120atcctgaagt
tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg
180ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct
ctctatggcc 240tacgccatgt acaccattgg tgtcatgtcc gacaactgcg
agaaggcttt cgacaacaat 300gtcttccgaa tcaccactca gctgttctac
ctcagcaagt tcctcgagta cattgactcc 360ttctatctgc ccctcatggg
caagcctctg acctggttgc agttctttca ccatctcgga 420gctcctatgg
acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg
480ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg
actgatcaag 540ctcaagttcc ctatgcccaa gtccctgatt acttctatgc
agatcattca gttcaacgtt 600ggcttctaca tcgtctggaa gtaccggaac
attccctgct accgacaaga tggaatgaga 660atgtttggct ggtttttcaa
ctacttctac gttggtactg tcctgtgtct gttcctcaac 720ttctacgtgc
agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga
777673497DNAArtificial Sequenceplasmid pEgD9ES 67tcgcgcgttt
cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60cagcttgtct
gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
120ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
ctgagagtgc 180accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
aaaataccgc atcaggcgcc 240attcgccatt caggctgcgc aactgttggg
aagggcgatc ggtgcgggcc tcttcgctat 300tacgccagct ggcgaaaggg
ggatgtgctg caaggcgatt aagttgggta acgccagggt 360tttcccagtc
acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
420tgcatctaga tccatggagg tcgtgaacga aatcgtctcc attggccagg
aggttcttcc 480caaggtcgac tatgctcagc tctggtctga tgcctcgcac
tgcgaggtgc tgtacctctc 540catcgccttc gtcatcctga agttcaccct
tggtcctctc ggacccaagg gtcagtctcg 600aatgaagttt gtgttcacca
actacaacct gctcatgtcc atctactcgc tgggctcctt 660cctctctatg
gcctacgcca tgtacaccat tggtgtcatg tccgacaact gcgagaaggc
720tttcgacaac aatgtcttcc gaatcaccac tcagctgttc tacctcagca
agttcctcga 780gtacattgac tccttctatc tgcccctcat gggcaagcct
ctgacctggt tgcagttctt 840tcaccatctc ggagctccta tggacatgtg
gctgttctac aactaccgaa acgaagccgt 900ttggatcttt gtgctgctca
acggcttcat tcactggatc atgtacggct actattggac 960ccgactgatc
aagctcaagt tccctatgcc caagtccctg attacttcta tgcagatcat
1020tcagttcaac gttggcttct acatcgtctg gaagtaccgg aacattccct
gctaccgaca 1080agatggaatg agaatgtttg gctggttttt caactacttc
tacgttggta ctgtcctgtg 1140tctgttcctc aacttctacg tgcagaccta
catcgtccga aagcacaagg gagccaaaaa 1200gattcagtga gcggccgcat
cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag 1260cttggcgtaa
tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
1320acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
gagtgagcta 1380actcacatta attgcgttgc gctcactgcc cgctttccag
tcgggaaacc tgtcgtgcca 1440gctgcattaa tgaatcggcc aacgcgcggg
gagaggcggt ttgcgtattg ggcgctcttc 1500cgcttcctcg ctcactgact
cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 1560tcactcaaag
gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
1620gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
tggcgttttt 1680ccataggctc cgcccccctg acgagcatca caaaaatcga
cgctcaagtc agaggtggcg 1740aaacccgaca ggactataaa gataccaggc
gtttccccct ggaagctccc tcgtgcgctc 1800tcctgttccg accctgccgc
ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1860ggcgctttct
catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
1920gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
ccggtaacta 1980tcgtcttgag tccaacccgg taagacacga cttatcgcca
ctggcagcag ccactggtaa 2040caggattagc agagcgaggt atgtaggcgg
tgctacagag ttcttgaagt ggtggcctaa 2100ctacggctac actagaagaa
cagtatttgg tatctgcgct ctgctgaagc cagttacctt 2160cggaaaaaga
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2220ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
atcctttgat 2280cttttctacg gggtctgacg ctcagtggaa cgaaaactca
cgttaaggga ttttggtcat 2340gagattatca aaaaggatct tcacctagat
ccttttaaat taaaaatgaa gttttaaatc 2400aatctaaagt atatatgagt
aaacttggtc tgacagttac caatgcttaa tcagtgaggc 2460acctatctca
gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
2520gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
taccgcgaga 2580cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa gggccgagcg 2640cagaagtggt cctgcaactt tatccgcctc
catccagtct attaattgtt gccgggaagc 2700tagagtaagt agttcgccag
ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 2760cgtggtgtca
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
2820gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
gtcctccgat 2880cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
gttatggcag cactgcataa 2940ttctcttact gtcatgccat ccgtaagatg
cttttctgtg actggtgagt actcaaccaa 3000gtcattctga gaatagtgta
tgcggcgacc gagttgctct tgcccggcgt caatacggga 3060taataccgcg
ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3120gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
ccactcgtgc 3180acccaactga tcttcagcat cttttacttt caccagcgtt
tctgggtgag caaaaacagg 3240aaggcaaaat gccgcaaaaa agggaataag
ggcgacacgg aaatgttgaa tactcatact 3300cttccttttt caatattatt
gaagcattta tcagggttat tgtctcatga gcggatacat 3360atttgaatgt
atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3420gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
ataggcgtat 3480cacgaggccc tttcgtc 3497689472DNAArtificial
Sequenceplasmid pDMW263 68catggcatgg atggtacgtc ctgtagaaac
cccaacccgt gaaatcaaaa aactcgacgg 60cctgtgggca ttcagtctgg atcgcgaaaa
ctgtggaatt gatcagcgtt ggtgggaaag 120cgcgttacaa gaaagccggg
caattgctgt gccaggcagt tttaacgatc agttcgccga 180tgcagatatt
cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
240aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
acggcaaagt 300gtgggtcaat aatcaggaag tgatggagca tcagggcggc
tatacgccat ttgaagccga 360tgtcacgccg tatgttattg ccgggaaaag
tgtacgtatc accgtttgtg tgaacaacga 420actgaactgg cagactatcc
cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa 480gcagtcttac
ttccatgatt tctttaacta tgccgggatc catcgcagcg taatgctcta
540caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
cgcaagactg 600taaccacgcg tctgttgact ggcaggtggt ggccaatggt
gatgtcagcg ttgaactgcg 660tgatgcggat caacaggtgg ttgcaactgg
acaaggcact agcgggactt tgcaagtggt 720gaatccgcac ctctggcaac
cgggtgaagg ttatctctat gaactgtgcg tcacagccaa 780aagccagaca
gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
840gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
gtcgtcatga 900agatgcggac ttacgtggca aaggattcga taacgtgctg
atggtgcacg accacgcatt 960aatggactgg attggggcca actcctaccg
tacctcgcat tacccttacg ctgaagagat 1020gctcgactgg gcagatgaac
atggcatcgt ggtgattgat gaaactgctg ctgtcggctt 1080taacctctct
ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
1140agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
agctgatagc 1200gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
gccaacgaac cggatacccg 1260tccgcaagtg cacgggaata tttcgccact
ggcggaagca acgcgtaaac tcgacccgac 1320gcgtccgatc acctgcgtca
atgtaatgtt ctgcgacgct cacaccgata ccatcagcga 1380tctctttgat
gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt
1440ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga
aactgcatca 1500gccgattatc atcaccgaat acggcgtgga tacgttagcc
gggctgcact caatgtacac 1560cgacatgtgg agtgaagagt atcagtgtgc
atggctggat atgtatcacc gcgtctttga 1620tcgcgtcagc gccgtcgtcg
gtgaacaggt atggaatttc gccgattttg cgacctcgca 1680aggcatattg
cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa
1740gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg
aaaaaccgca 1800gcagggaggc aaacaatgat taattaacta gagcggccgc
caccgcggcc cgagattccg 1860gcctcttcgg ccgccaagcg acccgggtgg
acgtctagag gtacctagca attaacagat 1920agtttgccgg tgataattct
cttaacctcc cacactcctt tgacataacg atttatgtaa 1980cgaaactgaa
atttgaccag atattgtgtc cgcggtggag ctccagcttt tgttcccttt
2040agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
gtgtgaaatt 2100gttatccgct cacaattcca cacaacatac gagccggaag
cataaagtgt aaagcctggg 2160gtgcctaatg agtgagctaa ctcacattaa
ttgcgttgcg ctcactgccc gctttccagt 2220cgggaaacct gtcgtgccag
ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 2280tgcgtattgg
gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2340tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
gaatcagggg 2400ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
ggccaggaac cgtaaaaagg 2460ccgcgttgct ggcgtttttc cataggctcc
gcccccctga cgagcatcac aaaaatcgac 2520gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg 2580gaagctccct
cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
2640ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
ctcagttcgg 2700tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
ccccgttcag cccgaccgct 2760gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac ttatcgccac 2820tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt gctacagagt 2880tcttgaagtg
gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
2940tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
aaacaaacca 3000ccgctggtag cggtggtttt tttgtttgca agcagcagat
tacgcgcaga aaaaaaggat 3060ctcaagaaga tcctttgatc ttttctacgg
ggtctgacgc tcagtggaac gaaaactcac 3120gttaagggat tttggtcatg
agattatcaa aaaggatctt cacctagatc cttttaaatt 3180aaaaatgaag
ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3240aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
tccatagttg 3300cctgactccc cgtcgtgtag ataactacga tacgggaggg
cttaccatct ggccccagtg 3360ctgcaatgat accgcgagac ccacgctcac
cggctccaga tttatcagca ataaaccagc 3420cagccggaag ggccgagcgc
agaagtggtc ctgcaacttt atccgcctcc atccagtcta 3480ttaattgttg
ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
3540ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
tcattcagct 3600ccggttccca acgatcaagg cgagttacat gatcccccat
gttgtgcaaa aaagcggtta 3660gctccttcgg tcctccgatc gttgtcagaa
gtaagttggc cgcagtgtta tcactcatgg 3720ttatggcagc actgcataat
tctcttactg tcatgccatc cgtaagatgc ttttctgtga 3780ctggtgagta
ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
3840gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
gtgctcatca 3900ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
accgctgttg agatccagtt 3960cgatgtaacc cactcgtgca cccaactgat
cttcagcatc ttttactttc accagcgttt 4020ctgggtgagc aaaaacagga
aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4080aatgttgaat
actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
4140gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
ggggttccgc 4200gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
cggcgcatta agcgcggcgg 4260gtgtggtggt tacgcgcagc gtgaccgcta
cacttgccag cgccctagcg cccgctcctt 4320tcgctttctt cccttccttt
ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 4380gggggctccc
tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4440attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
cgccctttga 4500cgttggagtc cacgttcttt aatagtggac tcttgttcca
aactggaaca acactcaacc 4560ctatctcggt ctattctttt gatttataag
ggattttgcc gatttcggcc tattggttaa 4620aaaatgagct gatttaacaa
aaatttaacg cgaattttaa caaaatatta acgcttacaa 4680tttccattcg
ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
4740gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
gggtaacgcc 4800agggttttcc cagtcacgac gttgtaaaac gacggccagt
gaattgtaat acgactcact 4860atagggcgaa ttgggtaccg ggccccccct
cgaggtcgat ggtgtcgata agcttgatat 4920cgaattcatg tcacacaaac
cgatcttcgc ctcaaggaaa cctaattcta catccgagag 4980actgccgaga
tccagtctac actgattaat tttcgggcca ataatttaaa aaaatcgtgt
5040tatataatat tatatgtatt atatatatac atcatgatga
tactgacagt catgtcccat 5100tgctaaatag acagactcca tctgccgcct
ccaactgatg ttctcaatat ttaaggggtc 5160atctcgcatt gtttaataat
aaacagactc catctaccgc ctccaaatga tgttctcaaa 5220atatattgta
tgaacttatt tttattactt agtattatta gacaacttac ttgctttatg
5280aaaaacactt cctatttagg aaacaattta taatggcagt tcgttcattt
aacaatttat 5340gtagaataaa tgttataaat gcgtatggga aatcttaaat
atggatagca taaatgatat 5400ctgcattgcc taattcgaaa tcaacagcaa
cgaaaaaaat cccttgtaca acataaatag 5460tcatcgagaa atatcaacta
tcaaagaaca gctattcaca cgttactatt gagattatta 5520ttggacgaga
atcacacact caactgtctt tctctcttct agaaatacag gtacaagtat
5580gtactattct cattgttcat acttctagtc atttcatccc acatattcct
tggatttctc 5640tccaatgaat gacattctat cttgcaaatt caacaattat
aataagatat accaaagtag 5700cggtatagtg gcaatcaaaa agcttctctg
gtgtgcttct cgtatttatt tttattctaa 5760tgatccatta aaggtatata
tttatttctt gttatataat ccttttgttt attacatggg 5820ctggatacat
aaaggtattt tgatttaatt ttttgcttaa attcaatccc ccctcgttca
5880gtgtcaactg taatggtagg aaattaccat acttttgaag aagcaaaaaa
aatgaaagaa 5940aaaaaaaatc gtatttccag gttagacgtt ccgcagaatc
tagaatgcgg tatgcggtac 6000attgttcttc gaacgtaaaa gttgcgctcc
ctgagatatt gtacattttt gcttttacaa 6060gtacaagtac atcgtacaac
tatgtactac tgttgatgca tccacaacag tttgttttgt 6120ttttttttgt
tttttttttt tctaatgatt cattaccgct atgtatacct acttgtactt
6180gtagtaagcc gggttattgg cgttcaatta atcatagact tatgaatctg
cacggtgtgc 6240gctgcgagtt acttttagct tatgcatgct acttgggtgt
aatattggga tctgttcgga 6300aatcaacgga tgctcaaccg atttcgacag
taataatttg aatcgaatcg gagcctaaaa 6360tgaacccgag tatatctcat
aaaattctcg gtgagaggtc tgtgactgtc agtacaaggt 6420gccttcatta
tgccctcaac cttaccatac ctcactgaat gtagtgtacc tctaaaaatg
6480aaatacagtg ccaaaagcca aggcactgag ctcgtctaac ggacttgata
tacaaccaat 6540taaaacaaat gaaaagaaat acagttcttt gtatcatttg
taacaattac cctgtacaaa 6600ctaaggtatt gaaatcccac aatattccca
aagtccaccc ctttccaaat tgtcatgcct 6660acaactcata taccaagcac
taacctacca aacaccacta aaaccccaca aaatatatct 6720taccgaatat
acagtaacaa gctaccacca cactcgttgg gtgcagtcgc cagcttaaag
6780atatctatcc acatcagcca caactccctt cctttaataa accgactaca
cccttggcta 6840ttgaggttat gagtgaatat actgtagaca agacactttc
aagaagactg tttccaaaac 6900gtaccactgt cctccactac aaacacaccc
aatctgcttc ttctagtcaa ggttgctaca 6960ccggtaaatt ataaatcatc
atttcattag cagggcaggg ccctttttat agagtcttat 7020acactagcgg
accctgccgg tagaccaacc cgcaggcgcg tcagtttgct ccttccatca
7080atgcgtcgta gaaacgactt actccttctt gagcagctcc ttgaccttgt
tggcaacaag 7140tctccgacct cggaggtgga ggaagagcct ccgatatcgg
cggtagtgat accagcctcg 7200acggactcct tgacggcagc ctcaacagcg
tcaccggcgg gcttcatgtt aagagagaac 7260ttgagcatca tggcggcaga
cagaatggtg gcaatggggt tgaccttctg cttgccgaga 7320tcgggggcag
atccgtgaca gggctcgtac agaccgaacg cctcgttggt gtcgggcaga
7380gaagccagag aggcggaggg cagcagaccc agagaaccgg ggatgacgga
ggcctcgtcg 7440gagatgatat cgccaaacat gttggtggtg atgatgatac
cattcatctt ggagggctgc 7500ttgatgagga tcatggcggc cgagtcgatc
agctggtggt tgagctcgag ctgggggaat 7560tcgtccttga ggactcgagt
gacagtcttt cgccaaagtc gagaggaggc cagcacgttg 7620gccttgtcaa
gagaccacac gggaagaggg gggttgtgct gaagggccag gaaggcggcc
7680attcgggcaa ttcgctcaac ctcaggaacg gagtaggtct cggtgtcgga
agcgacgcca 7740gatccgtcat cctcctttcg ctctccaaag tagatacctc
cgacgagctc tcggacaatg 7800atgaagtcgg tgccctcaac gtttcggatg
ggggagagat cggcgagctt gggcgacagc 7860agctggcagg gtcgcaggtt
ggcgtacagg ttcaggtcct ttcgcagctt gaggagaccc 7920tgctcgggtc
gcacgtcggt tcgtccgtcg ggagtggtcc atacggtgtt ggcagcgcct
7980ccgacagcac cgagcataat agagtcagcc tttcggcaga tgtcgagagt
agcgtcggtg 8040atgggctcgc cctccttctc aatggcagct cctccaatga
gtcggtcctc aaacacaaac 8100tcggtgccgg aggcctcagc aacagacttg
agcaccttga cggcctcggc aatcacctcg 8160gggccacaga agtcgccgcc
gagaagaaca atcttcttgg agtcagtctt ggtcttctta 8220gtttcgggtt
ccattgtgga tgtgtgtggt tgtatgtgtg atgtggtgtg tggagtgaaa
8280atctgtggct ggcaaacgct cttgtatata tacgcacttt tgcccgtgct
atgtggaaga 8340ctaaacctcc gaagattgtg actcaggtag tgcggtatcg
gctagggacc caaaccttgt 8400cgatgccgat agcgctatcg aacgtacccc
agccggccgg gagtatgtcg gaggggacat 8460acgagatcgt caagggtttg
tggccaactg gtaaataaat gatgtcgacg tttaaacagt 8520gtacgcagat
ctactataga ggaacattta aattgccccg gagaagacgg ccaggccgcc
8580tagatgacaa attcaacaac tcacagctga ctttctgcca ttgccactag
gggggggcct 8640ttttatatgg ccaagccaag ctctccacgt cggttgggct
gcacccaaca ataaatgggt 8700agggttgcac caacaaaggg atgggatggg
gggtagaaga tacgaggata acggggctca 8760atggcacaaa taagaacgaa
tactgccatt aagactcgtg atccagcgac tgacaccatt 8820gcatcatcta
agggcctcaa aactacctcg gaactgctgc gctgatctgg acaccacaga
8880ggttccgagc actttaggtt gcaccaaatg tcccaccagg tgcaggcaga
aaacgctgga 8940acagcgtgta cagtttgtct taacaaaaag tgagggcgct
gaggtcgagc agggtggtgt 9000gacttgttat agcctttaga gctgcgaaag
cgcgtatgga tttggctcat caggccagat 9060tgagggtctg tggacacatg
tcatgttagt gtacttcaat cgccccctgg atatagcccc 9120gacaataggc
cgtggcctca tttttttgcc ttccgcacat ttccattgct cgatacccac
9180accttgcttc tcctgcactt gccaacctta atactggttt acattgacca
acatcttaca 9240agcggggggc ttgtctaggg tatatataaa cagtggctct
cccaatcggt tgccagtctc 9300ttttttcctt tctttcccca cagattcgaa
atctaaacta cacatcacag aattccgagc 9360cgtgagtatc cacgacaaga
tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc 9420gaaagtcgct
agcaacacac actctctaca caaactaacc cagctctggt ac
9472698165DNAArtificial Sequenceplasmid pZUF17 69gtacgagccg
gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 60ttaattgcgt
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
120taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
ttccgcttcc 180tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
gagcggtatc agctcactca 240aaggcggtaa tacggttatc cacagaatca
ggggataacg caggaaagaa catgtgagca 300aaaggccagc aaaaggccag
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 360ctccgccccc
ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
420acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt 480ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt 540tctcatagct cacgctgtag gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc 600tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 660gagtccaacc
cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
720agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc 780tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa 840agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt 900tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct 960acggggtctg
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1020tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
atcaatctaa 1080agtatatatg agtaaacttg gtctgacagt taccaatgct
taatcagtga ggcacctatc 1140tcagcgatct gtctatttcg ttcatccata
gttgcctgac tccccgtcgt gtagataact 1200acgatacggg agggcttacc
atctggcccc agtgctgcaa tgataccgcg agacccacgc 1260tcaccggctc
cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
1320ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
agctagagta 1380agtagttcgc cagttaatag tttgcgcaac gttgttgcca
ttgctacagg catcgtggtg 1440tcacgctcgt cgtttggtat ggcttcattc
agctccggtt cccaacgatc aaggcgagtt 1500acatgatccc ccatgttgtg
caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1560agaagtaagt
tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
1620actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
caagtcattc 1680tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc 1740gcgccacata gcagaacttt aaaagtgctc
atcattggaa aacgttcttc ggggcgaaaa 1800ctctcaagga tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac 1860tgatcttcag
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
1920aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
actcttcctt 1980tttcaatatt attgaagcat ttatcagggt tattgtctca
tgagcggata catatttgaa 2040tgtatttaga aaaataaaca aataggggtt
ccgcgcacat ttccccgaaa agtgccacct 2100gacgcgccct gtagcggcgc
attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 2160gctacacttg
ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
2220acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
gttccgattt 2280agtgctttac ggcacctcga ccccaaaaaa cttgattagg
gtgatggttc acgtagtggg 2340ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt ctttaatagt 2400ggactcttgt tccaaactgg
aacaacactc aaccctatct cggtctattc ttttgattta 2460taagggattt
tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2520aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
aggctgcgca 2580actgttggga agggcgatcg gtgcgggcct cttcgctatt
acgccagctg gcgaaagggg 2640gatgtgctgc aaggcgatta agttgggtaa
cgccagggtt ttcccagtca cgacgttgta 2700aaacgacggc cagtgaattg
taatacgact cactataggg cgaattgggt accgggcccc 2760ccctcgaggt
cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct
2820tcgcctcaag gaaacctaat tctacatccg agagactgcc gagatccagt
ctacactgat 2880taattttcgg gccaataatt taaaaaaatc gtgttatata
atattatatg tattatatat 2940atacatcatg atgatactga cagtcatgtc
ccattgctaa atagacagac tccatctgcc 3000gcctccaact gatgttctca
atatttaagg ggtcatctcg cattgtttaa taataaacag 3060actccatcta
ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt
3120acttagtatt attagacaac ttacttgctt tatgaaaaac acttcctatt
taggaaacaa 3180tttataatgg cagttcgttc atttaacaat ttatgtagaa
taaatgttat aaatgcgtat 3240gggaaatctt aaatatggat agcataaatg
atatctgcat tgcctaattc gaaatcaaca 3300gcaacgaaaa aaatcccttg
tacaacataa atagtcatcg agaaatatca actatcaaag 3360aacagctatt
cacacgttac tattgagatt attattggac gagaatcaca cactcaactg
3420tctttctctc ttctagaaat acaggtacaa gtatgtacta ttctcattgt
tcatacttct 3480agtcatttca tcccacatat tccttggatt tctctccaat
gaatgacatt ctatcttgca 3540aattcaacaa ttataataag atataccaaa
gtagcggtat agtggcaatc aaaaagcttc 3600tctggtgtgc ttctcgtatt
tatttttatt ctaatgatcc attaaaggta tatatttatt 3660tcttgttata
taatcctttt gtttattaca tgggctggat acataaaggt attttgattt
3720aattttttgc ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg
taggaaatta 3780ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa
aatcgtattt ccaggttaga 3840cgttccgcag aatctagaat gcggtatgcg
gtacattgtt cttcgaacgt aaaagttgcg 3900ctccctgaga tattgtacat
ttttgctttt acaagtacaa gtacatcgta caactatgta 3960ctactgttga
tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat
4020gattcattac cgctatgtat acctacttgt acttgtagta agccgggtta
ttggcgttca 4080attaatcata gacttatgaa tctgcacggt gtgcgctgcg
agttactttt agcttatgca 4140tgctacttgg gtgtaatatt gggatctgtt
cggaaatcaa cggatgctca atcgatttcg 4200acagtaatta attaagtcat
acacaagtca gctttcttcg agcctcatat aagtataagt 4260agttcaacgt
attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc
4320cattggacag atcatgcgga tacacaggtt gtgcagtatc atacatactc
gatcagacag 4380gtcgtctgac catcatacaa gctgaacaag cgctccatac
ttgcacgctc tctatataca 4440cagttaaatt acatatccat agtctaacct
ctaacagtta atcttctggt aagcctccca 4500gccagccttc tggtatcgct
tggcctcctc aataggatct cggttctggc cgtacagacc 4560tcggccgaca
attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg
4620ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa
taagccagtc 4680ctcagagtcg cccttaggtc ggttctgggc aatgaagcca
accacaaact cggggtcgga 4740tcgggcaagc tcaatggtct gcttggagta
ctcgccagtg gccagagagc ccttgcaaga 4800cagctcggcc agcatgagca
gacctctggc cagcttctcg ttgggagagg ggactaggaa 4860ctccttgtac
tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt
4920ttcctcggca ccagctcgca ggccagcaat gattccggtt ccgggtacac
cgtgggcgtt 4980ggtgatatcg gaccactcgg cgattcggtg acaccggtac
tggtgcttga cagtgttgcc 5040aatatctgcg aactttctgt cctcgaacag
gaagaaaccg tgcttaagag caagttcctt 5100gagggggagc acagtgccgg
cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat 5160catgcacaca
taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac
5220atccagagaa gcacacaggt tggttttctt ggctgccacg agcttgagca
ctcgagcggc 5280aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag
ggcattttgg tggtgaagag 5340gagactgaaa taaatttagt ctgcagaact
ttttatcgga accttatctg gggcagtgaa 5400gtatatgtta tggtaatagt
tacgagttag ttgaacttat agatagactg gactatacgg 5460ctatcggtcc
aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa
5520aatgtgatca tgatgaaagc cagcaatgac gttgcagctg atattgttgt
cggccaaccg 5580cgccgaaaac gcagctgtca gacccacagc ctccaacgaa
gaatgtatcg tcaaagtgat 5640ccaagcacac tcatagttgg agtcgtactc
caaaggcggc aatgacgagt cagacagata 5700ctcgtcgact caggcgacga
cggaattcct gcagcccatc tgcagaattc aggagagacc 5760gggttggcgg
cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca
5820ggccgcctag atgacaaatt caacaactca cagctgactt tctgccattg
ccactagggg 5880ggggcctttt tatatggcca agccaagctc tccacgtcgg
ttgggctgca cccaacaata 5940aatgggtagg gttgcaccaa caaagggatg
ggatgggggg tagaagatac gaggataacg 6000gggctcaatg gcacaaataa
gaacgaatac tgccattaag actcgtgatc cagcgactga 6060caccattgca
tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca
6120ccacagaggt tccgagcact ttaggttgca ccaaatgtcc caccaggtgc
aggcagaaaa 6180cgctggaaca gcgtgtacag tttgtcttaa caaaaagtga
gggcgctgag gtcgagcagg 6240gtggtgtgac ttgttatagc ctttagagct
gcgaaagcgc gtatggattt ggctcatcag 6300gccagattga gggtctgtgg
acacatgtca tgttagtgta cttcaatcgc cccctggata 6360tagccccgac
aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg
6420tacccacacc ttgcttctcc tgcacttgcc aaccttaata ctggtttaca
ttgaccaaca 6480tcttacaagc ggggggcttg tctagggtat atataaacag
tggctctccc aatcggttgc 6540cagtctcttt tttcctttct ttccccacag
attcgaaatc taaactacac atcacacaat 6600gcctgttact gacgtcctta
agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc 6660cgtgagtatc
cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc
6720gaaagtcgct agcaacacac actctctaca caaactaacc cagctctcca
tggctgagga 6780taagaccaag gtcgagttcc ctaccctgac tgagctgaag
cactctatcc ctaacgcttg 6840ctttgagtcc aacctcggac tctcgctcta
ctacactgcc cgagcgatct tcaacgcatc 6900tgcctctgct gctctgctct
acgctgcccg atctactccc ttcattgccg ataacgttct 6960gctccacgct
ctggtttgcg ccacctacat ctacgtgcag ggtgtcatct tctggggttt
7020ctttaccgtc ggtcacgact gtggtcactc tgccttctcc cgataccact
ccgtcaactt 7080catcattggc tgcatcatgc actctgccat tctgactccc
ttcgagtcct ggcgagtgac 7140ccaccgacac catcacaaga acactggcaa
cattgataag gacgagatct tctaccctca 7200tcggtccgtc aaggacctcc
aggacgtgcg acaatgggtc tacaccctcg gaggtgcttg 7260gtttgtctac
ctgaaggtcg gatatgctcc tcgaaccatg tcccactttg acccctggga
7320ccctctcctg cttcgacgag cctccgctgt catcgtgtcc ctcggagtct
gggctgcctt 7380cttcgctgcc tacgcctacc tcacatactc gctcggcttt
gccgtcatgg gcctctacta 7440ctatgctcct ctctttgtct ttgcttcgtt
cctcgtcatt actaccttct tgcatcacaa 7500cgacgaagct actccctggt
acggtgactc ggagtggacc tacgtcaagg gcaacctgag 7560ctccgtcgac
cgatcgtacg gagctttcgt ggacaacctg tctcaccaca ttggcaccca
7620ccaggtccat cacttgttcc ctatcattcc ccactacaag ctcaacgaag
ccaccaagca 7680ctttgctgcc gcttaccctc acctcgtgag acgtaacgac
gagcccatca ttactgcctt 7740cttcaagacc gctcacctct ttgtcaacta
cggagctgtg cccgagactg ctcagatttt 7800caccctcaaa gagtctgccg
ctgcagccaa ggccaagagc gactaagcgg ccgcaagtgt 7860ggatggggaa
gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt
7920caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg
atatttatgt 7980ttgacacttg agaatgtacg atacaagcac tgtccaagta
caatactaaa catactgtac 8040atactcatac tcgtacccgg gcaacggttt
cacttgagtg cagtggctag tgctcttact 8100cgtacagtgt gcaatactgc
gtatcatagt ctttgatgta tatcgtattc attcatgtta 8160gttgc
8165707769DNAArtificial Sequenceplasmid pZUFmEgD9ES 70ggccgcaagt
gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa 60gatggatgga
ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac
120ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag
tacaatacta 180aacatactgt acatactcat actcgtaccc gggcaacggt
ttcacttgag tgcagtggct 240agtgctctta ctcgtacagt gtgcaatact
gcgtatcata gtctttgatg tatatcgtat 300tcattcatgt tagttgcgta
cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 360gagtgagcta
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
420tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
ttgcgtattg 480ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
ggtcgttcgg ctgcggcgag 540cggtatcagc tcactcaaag gcggtaatac
ggttatccac agaatcaggg gataacgcag 600gaaagaacat gtgagcaaaa
ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 660tggcgttttt
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
720agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
ggaagctccc 780tcgtgcgctc tcctgttccg accctgccgc ttaccggata
cctgtccgcc tttctccctt 840cgggaagcgt ggcgctttct catagctcac
gctgtaggta tctcagttcg gtgtaggtcg 900ttcgctccaa gctgggctgt
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 960ccggtaacta
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
1020ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
ttcttgaagt 1080ggtggcctaa ctacggctac actagaagga cagtatttgg
tatctgcgct ctgctgaagc 1140cagttacctt cggaaaaaga gttggtagct
cttgatccgg caaacaaacc accgctggta 1200gcggtggttt ttttgtttgc
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 1260atcctttgat
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
1320ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
taaaaatgaa 1380gttttaaatc aatctaaagt atatatgagt aaacttggtc
tgacagttac caatgcttaa 1440tcagtgaggc acctatctca gcgatctgtc
tatttcgttc atccatagtt gcctgactcc 1500ccgtcgtgta gataactacg
atacgggagg gcttaccatc tggccccagt gctgcaatga 1560taccgcgaga
cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
1620gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
attaattgtt 1680gccgggaagc tagagtaagt agttcgccag ttaatagttt
gcgcaacgtt gttgccattg 1740ctacaggcat cgtggtgtca cgctcgtcgt
ttggtatggc ttcattcagc tccggttccc 1800aacgatcaag gcgagttaca
tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1860gtcctccgat
cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
1920cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
actggtgagt 1980actcaaccaa gtcattctga gaatagtgta tgcggcgacc
gagttgctct tgcccggcgt 2040caatacggga taataccgcg ccacatagca
gaactttaaa agtgctcatc attggaaaac 2100gttcttcggg gcgaaaactc
tcaaggatct taccgctgtt gagatccagt tcgatgtaac 2160ccactcgtgc
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
2220caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
aaatgttgaa 2280tactcatact cttccttttt caatattatt gaagcattta
tcagggttat tgtctcatga 2340gcggatacat
atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
2400cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
ggtgtggtgg 2460ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
gcccgctcct ttcgctttct 2520tcccttcctt tctcgccacg ttcgccggct
ttccccgtca agctctaaat cgggggctcc 2580ctttagggtt ccgatttagt
gctttacggc acctcgaccc caaaaaactt gattagggtg 2640atggttcacg
tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
2700ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
cctatctcgg 2760tctattcttt tgatttataa gggattttgc cgatttcggc
ctattggtta aaaaatgagc 2820tgatttaaca aaaatttaac gcgaatttta
acaaaatatt aacgcttaca atttccattc 2880gccattcagg ctgcgcaact
gttgggaagg gcgatcggtg cgggcctctt cgctattacg 2940ccagctggcg
aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
3000ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
tatagggcga 3060attgggtacc gggccccccc tcgaggtcga tggtgtcgat
aagcttgata tcgaattcat 3120gtcacacaaa ccgatcttcg cctcaaggaa
acctaattct acatccgaga gactgccgag 3180atccagtcta cactgattaa
ttttcgggcc aataatttaa aaaaatcgtg ttatataata 3240ttatatgtat
tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata
3300gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt
catctcgcat 3360tgtttaataa taaacagact ccatctaccg cctccaaatg
atgttctcaa aatatattgt 3420atgaacttat ttttattact tagtattatt
agacaactta cttgctttat gaaaaacact 3480tcctatttag gaaacaattt
ataatggcag ttcgttcatt taacaattta tgtagaataa 3540atgttataaa
tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc
3600ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata
gtcatcgaga 3660aatatcaact atcaaagaac agctattcac acgttactat
tgagattatt attggacgag 3720aatcacacac tcaactgtct ttctctcttc
tagaaataca ggtacaagta tgtactattc 3780tcattgttca tacttctagt
catttcatcc cacatattcc ttggatttct ctccaatgaa 3840tgacattcta
tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt
3900ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta
atgatccatt 3960aaaggtatat atttatttct tgttatataa tccttttgtt
tattacatgg gctggataca 4020taaaggtatt ttgatttaat tttttgctta
aattcaatcc cccctcgttc agtgtcaact 4080gtaatggtag gaaattacca
tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat 4140cgtatttcca
ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt
4200cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca
agtacaagta 4260catcgtacaa ctatgtacta ctgttgatgc atccacaaca
gtttgttttg tttttttttg 4320tttttttttt ttctaatgat tcattaccgc
tatgtatacc tacttgtact tgtagtaagc 4380cgggttattg gcgttcaatt
aatcatagac ttatgaatct gcacggtgtg cgctgcgagt 4440tacttttagc
ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg
4500atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct
ttcttcgagc 4560ctcatataag tataagtagt tcaacgtatt agcactgtac
ccagcatctc cgtatcgaga 4620aacacaacaa catgccccat tggacagatc
atgcggatac acaggttgtg cagtatcata 4680catactcgat cagacaggtc
gtctgaccat catacaagct gaacaagcgc tccatacttg 4740cacgctctct
atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc
4800ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat
aggatctcgg 4860ttctggccgt acagacctcg gccgacaatt atgatatccg
ttccggtaga catgacatcc 4920tcaacagttc ggtactgctg tccgagagcg
tctcccttgt cgtcaagacc caccccgggg 4980gtcagaataa gccagtcctc
agagtcgccc ttaggtcggt tctgggcaat gaagccaacc 5040acaaactcgg
ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc
5100agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag
cttctcgttg 5160ggagagggga ctaggaactc cttgtactgg gagttctcgt
agtcagagac gtcctccttc 5220ttctgttcag agacagtttc ctcggcacca
gctcgcaggc cagcaatgat tccggttccg 5280ggtacaccgt gggcgttggt
gatatcggac cactcggcga ttcggtgaca ccggtactgg 5340tgcttgacag
tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc
5400ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
gtcaatgatg 5460tcgatatggg ttttgatcat gcacacataa ggtccgacct
tatcggcaag ctcaatgagc 5520tccttggtgg tggtaacatc cagagaagca
cacaggttgg ttttcttggc tgccacgagc 5580ttgagcactc gagcggcaaa
ggcggacttg tggacgttag ctcgagcttc gtaggagggc 5640attttggtgg
tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc
5700ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
aacttataga 5760tagactggac tatacggcta tcggtccaaa ttagaaagaa
cgtcaatggc tctctgggcg 5820tcgcctttgc cgacaaaaat gtgatcatga
tgaaagccag caatgacgtt gcagctgata 5880ttgttgtcgg ccaaccgcgc
cgaaaacgca gctgtcagac ccacagcctc caacgaagaa 5940tgtatcgtca
aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat
6000gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta
ctatagagga 6060acatttaaat tgccccggag aagacggcca ggccgcctag
atgacaaatt caacaactca 6120cagctgactt tctgccattg ccactagggg
ggggcctttt tatatggcca agccaagctc 6180tccacgtcgg ttgggctgca
cccaacaata aatgggtagg gttgcaccaa caaagggatg 6240ggatgggggg
tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac
6300tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg
gcctcaaaac 6360tacctcggaa ctgctgcgct gatctggaca ccacagaggt
tccgagcact ttaggttgca 6420ccaaatgtcc caccaggtgc aggcagaaaa
cgctggaaca gcgtgtacag tttgtcttaa 6480caaaaagtga gggcgctgag
gtcgagcagg gtggtgtgac ttgttatagc ctttagagct 6540gcgaaagcgc
gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca
6600tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt
ggcctcattt 6660ttttgccttc cgcacatttc cattgctcga tacccacacc
ttgcttctcc tgcacttgcc 6720aaccttaata ctggtttaca ttgaccaaca
tcttacaagc ggggggcttg tctagggtat 6780atataaacag tggctctccc
aatcggttgc cagtctcttt tttcctttct ttccccacag 6840attcgaaatc
taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca
6900gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc
aacacacact 6960ctctacacaa actaacccag ctctggtacc atggaggtcg
tgaacgaaat cgtctccatt 7020ggccaggagg ttcttcccaa ggtcgactat
gctcagctct ggtctgatgc ctcgcactgc 7080gaggtgctgt acctctccat
cgccttcgtc atcctgaagt tcacccttgg tcctctcgga 7140cccaagggtc
agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc
7200tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg
tgtcatgtcc 7260gacaactgcg agaaggcttt cgacaacaat gtcttccgaa
tcaccactca gctgttctac 7320ctcagcaagt tcctcgagta cattgactcc
ttctatctgc ccctcatggg caagcctctg 7380acctggttgc agttctttca
ccatctcgga gctcctatgg acatgtggct gttctacaac 7440taccgaaacg
aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg
7500tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa
gtccctgatt 7560acttctatgc agatcattca gttcaacgtt ggcttctaca
tcgtctggaa gtaccggaac 7620attccctgct accgacaaga tggaatgaga
atgtttggct ggtttttcaa ctacttctac 7680gttggtactg tcctgtgtct
gttcctcaac ttctacgtgc agacctacat cgtccgaaag 7740cacaagggag
ccaaaaagat tcagtgagc 77697110206DNAArtificial Sequenceplasmid
pZUFmE9SP8S 71taagtcatac acaagtcagc tttcttcgag cctcatataa
gtataagtag ttcaacgtat 60tagcactgta cccagcatct ccgtatcgag aaacacaaca
acatgcccca ttggacagat 120catgcggata cacaggttgt gcagtatcat
acatactcga tcagacaggt cgtctgacca 180tcatacaagc tgaacaagcg
ctccatactt gcacgctctc tatatacaca gttaaattac 240atatccatag
tctaacctct aacagttaat cttctggtaa gcctcccagc cagccttctg
300gtatcgcttg gcctcctcaa taggatctcg gttctggccg tacagacctc
ggccgacaat 360tatgatatcc gttccggtag acatgacatc ctcaacagtt
cggtactgct gtccgagagc 420gtctcccttg tcgtcaagac ccaccccggg
ggtcagaata agccagtcct cagagtcgcc 480cttaggtcgg ttctgggcaa
tgaagccaac cacaaactcg gggtcggatc gggcaagctc 540aatggtctgc
ttggagtact cgccagtggc cagagagccc ttgcaagaca gctcggccag
600catgagcaga cctctggcca gcttctcgtt gggagagggg actaggaact
ccttgtactg 660ggagttctcg tagtcagaga cgtcctcctt cttctgttca
gagacagttt cctcggcacc 720agctcgcagg ccagcaatga ttccggttcc
gggtacaccg tgggcgttgg tgatatcgga 780ccactcggcg attcggtgac
accggtactg gtgcttgaca gtgttgccaa tatctgcgaa 840ctttctgtcc
tcgaacagga agaaaccgtg cttaagagca agttccttga gggggagcac
900agtgccggcg taggtgaagt cgtcaatgat gtcgatatgg gttttgatca
tgcacacata 960aggtccgacc ttatcggcaa gctcaatgag ctccttggtg
gtggtaacat ccagagaagc 1020acacaggttg gttttcttgg ctgccacgag
cttgagcact cgagcggcaa aggcggactt 1080gtggacgtta gctcgagctt
cgtaggaggg cattttggtg gtgaagagga gactgaaata 1140aatttagtct
gcagaacttt ttatcggaac cttatctggg gcagtgaagt atatgttatg
1200gtaatagtta cgagttagtt gaacttatag atagactgga ctatacggct
atcggtccaa 1260attagaaaga acgtcaatgg ctctctgggc gtcgcctttg
ccgacaaaaa tgtgatcatg 1320atgaaagcca gcaatgacgt tgcagctgat
attgttgtcg gccaaccgcg ccgaaaacgc 1380agctgtcaga cccacagcct
ccaacgaaga atgtatcgtc aaagtgatcc aagcacactc 1440atagttggag
tcgtactcca aaggcggcaa tgacgagtca gacagatact cgtcgacgtt
1500taaacagtgt acgcagatct actatagagg aacatttaaa ttgccccgga
gaagacggcc 1560aggccgccta gatgacaaat tcaacaactc acagctgact
ttctgccatt gccactaggg 1620gggggccttt ttatatggcc aagccaagct
ctccacgtcg gttgggctgc acccaacaat 1680aaatgggtag ggttgcacca
acaaagggat gggatggggg gtagaagata cgaggataac 1740ggggctcaat
ggcacaaata agaacgaata ctgccattaa gactcgtgat ccagcgactg
1800acaccattgc atcatctaag ggcctcaaaa ctacctcgga actgctgcgc
tgatctggac 1860accacagagg ttccgagcac tttaggttgc accaaatgtc
ccaccaggtg caggcagaaa 1920acgctggaac agcgtgtaca gtttgtctta
acaaaaagtg agggcgctga ggtcgagcag 1980ggtggtgtga cttgttatag
cctttagagc tgcgaaagcg cgtatggatt tggctcatca 2040ggccagattg
agggtctgtg gacacatgtc atgttagtgt acttcaatcg ccccctggat
2100atagccccga caataggccg tggcctcatt tttttgcctt ccgcacattt
ccattgctcg 2160atacccacac cttgcttctc ctgcacttgc caaccttaat
actggtttac attgaccaac 2220atcttacaag cggggggctt gtctagggta
tatataaaca gtggctctcc caatcggttg 2280ccagtctctt ttttcctttc
tttccccaca gattcgaaat ctaaactaca catcacagaa 2340ttccgagccg
tgagtatcca cgacaagatc agtgtcgaga cgacgcgttt tgtgtaatga
2400cacaatccga aagtcgctag caacacacac tctctacaca aactaaccca
gctctggtac 2460catggaggtc gtgaacgaaa tcgtctccat tggccaggag
gttcttccca aggtcgacta 2520tgctcagctc tggtctgatg cctcgcactg
cgaggtgctg tacctctcca tcgccttcgt 2580catcctgaag ttcacccttg
gtcctctcgg acccaagggt cagtctcgaa tgaagtttgt 2640gttcaccaac
tacaacctgc tcatgtccat ctactcgctg ggctccttcc tctctatggc
2700ctacgccatg tacaccattg gtgtcatgtc cgacaactgc gagaaggctt
tcgacaacaa 2760tgtcttccga atcaccactc agctgttcta cctcagcaag
ttcctcgagt acattgactc 2820cttctatctg cccctcatgg gcaagcctct
gacctggttg cagttctttc accatctcgg 2880agctcctatg gacatgtggc
tgttctacaa ctaccgaaac gaagccgttt ggatctttgt 2940gctgctcaac
ggcttcattc actggatcat gtacggctac tattggaccc gactgatcaa
3000gctcaagttc cctatgccca agtccctgat tacttctatg cagatcattc
agttcaacgt 3060tggcttctac atcgtctgga agtaccggaa cattccctgc
taccgacaag atggaatgag 3120aatgtttggc tggtttttca actacttcta
cgttggtact gtcctgtgtc tgttcctcaa 3180cttctacgtg cagacctaca
tcgtccgaaa gcacaaggga gccaaaaaga ttcagtgagc 3240ggccgcaagt
gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa
3300gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg
atatagcaac 3360ggatatttat gtttgacact tgagaatgta cgatacaagc
actgtccaag tacaatacta 3420aacatactgt acatactcat actcgtaccc
gggcaacggt ttcacttgag tgcagtggct 3480agtgctctta ctcgtacagt
gtgcaatact gcgtatcata gtctttgatg tatatcgtat 3540tcattcatgt
tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
3600gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
tcgggaaacc 3660tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
gagaggcggt ttgcgtattg 3720ggcgctcttc cgcttcctcg ctcactgact
cgctgcgctc ggtcgttcgg ctgcggcgag 3780cggtatcagc tcactcaaag
gcggtaatac ggttatccac agaatcaggg gataacgcag 3840gaaagaacat
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
3900tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
cgctcaagtc 3960agaggtggcg aaacccgaca ggactataaa gataccaggc
gtttccccct ggaagctccc 4020tcgtgcgctc tcctgttccg accctgccgc
ttaccggata cctgtccgcc tttctccctt 4080cgggaagcgt ggcgctttct
catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4140ttcgctccaa
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4200ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
ctggcagcag 4260ccactggtaa caggattagc agagcgaggt atgtaggcgg
tgctacagag ttcttgaagt 4320ggtggcctaa ctacggctac actagaagga
cagtatttgg tatctgcgct ctgctgaagc 4380cagttacctt cggaaaaaga
gttggtagct cttgatccgg caaacaaacc accgctggta 4440gcggtggttt
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
4500atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
cgttaaggga 4560ttttggtcat gagattatca aaaaggatct tcacctagat
ccttttaaat taaaaatgaa 4620gttttaaatc aatctaaagt atatatgagt
aaacttggtc tgacagttac caatgcttaa 4680tcagtgaggc acctatctca
gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4740ccgtcgtgta
gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
4800taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa 4860gggccgagcg cagaagtggt cctgcaactt tatccgcctc
catccagtct attaattgtt 4920gccgggaagc tagagtaagt agttcgccag
ttaatagttt gcgcaacgtt gttgccattg 4980ctacaggcat cgtggtgtca
cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 5040aacgatcaag
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
5100gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
gttatggcag 5160cactgcataa ttctcttact gtcatgccat ccgtaagatg
cttttctgtg actggtgagt 5220actcaaccaa gtcattctga gaatagtgta
tgcggcgacc gagttgctct tgcccggcgt 5280caatacggga taataccgcg
ccacatagca gaactttaaa agtgctcatc attggaaaac 5340gttcttcggg
gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
5400ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
tctgggtgag 5460caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
ggcgacacgg aaatgttgaa 5520tactcatact cttccttttt caatattatt
gaagcattta tcagggttat tgtctcatga 5580gcggatacat atttgaatgt
atttagaaaa ataaacaaat aggggttccg cgcacatttc 5640cccgaaaagt
gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
5700ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
ttcgctttct 5760tcccttcctt tctcgccacg ttcgccggct ttccccgtca
agctctaaat cgggggctcc 5820ctttagggtt ccgatttagt gctttacggc
acctcgaccc caaaaaactt gattagggtg 5880atggttcacg tagtgggcca
tcgccctgat agacggtttt tcgccctttg acgttggagt 5940ccacgttctt
taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
6000tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
aaaaatgagc 6060tgatttaaca aaaatttaac gcgaatttta acaaaatatt
aacgcttaca atttccattc 6120gccattcagg ctgcgcaact gttgggaagg
gcgatcggtg cgggcctctt cgctattacg 6180ccagctggcg aaagggggat
gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 6240ccagtcacga
cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
6300attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata
tcgaattcat 6360gtcacacaaa ccgatcttcg cctcaaggaa acctaattct
acatccgaga gactgccgag 6420atccagtcta cactgattaa ttttcgggcc
aataatttaa aaaaatcgtg ttatataata 6480ttatatgtat tatatatata
catcatgatg atactgacag tcatgtccca ttgctaaata 6540gacagactcc
atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat
6600tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa
aatatattgt 6660atgaacttat ttttattact tagtattatt agacaactta
cttgctttat gaaaaacact 6720tcctatttag gaaacaattt ataatggcag
ttcgttcatt taacaattta tgtagaataa 6780atgttataaa tgcgtatggg
aaatcttaaa tatggatagc ataaatgata tctgcattgc 6840ctaattcgaa
atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga
6900aatatcaact atcaaagaac agctattcac acgttactat tgagattatt
attggacgag 6960aatcacacac tcaactgtct ttctctcttc tagaaataca
ggtacaagta tgtactattc 7020tcattgttca tacttctagt catttcatcc
cacatattcc ttggatttct ctccaatgaa 7080tgacattcta tcttgcaaat
tcaacaatta taataagata taccaaagta gcggtatagt 7140ggcaatcaaa
aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt
7200aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg
gctggataca 7260taaaggtatt ttgatttaat tttttgctta aattcaatcc
cccctcgttc agtgtcaact 7320gtaatggtag gaaattacca tacttttgaa
gaagcaaaaa aaatgaaaga aaaaaaaaat 7380cgtatttcca ggttagacgt
tccgcagaat ctagaatgcg gtatgcggta cattgttctt 7440cgaacgtaaa
agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta
7500catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg
tttttttttg 7560tttttttttt ttctaatgat tcattaccgc tatgtatacc
tacttgtact tgtagtaagc 7620cgggttattg gcgttcaatt aatcatagac
ttatgaatct gcacggtgtg cgctgcgagt 7680tacttttagc ttatgcatgc
tacttgggtg taatattggg atctgttcgg aaatcaacgg 7740atgctcaatc
gatggttaat gctgctgtgt gctgtgtgtg tgtgttgttt ggcgctcatt
7800gttgcgttat gcagcgtaca ccacaatatt ggaagcttat tagcctttct
attttttcgt 7860ttgcaaggct taacaacatt gctgtggaga gggatgggga
tatggaggcc gctggaggga 7920gtcggagagg cgttttggag cggcttggcc
tggcgcccag ctcgcgaaac gcacctagga 7980ccctttggca cgccgaaatg
tgccactttt cagtctagta acgccttacc tacgtcattc 8040catgcgtgca
tgtttgcgcc ttttttccct tgcccttgat cgccacacag tacagtgcac
8100tgtacagtgg aggttttggg ggggtcttag atgggagcta aaagcggcct
agcggtacac 8160tagtgggatt gtatggagtg gcatggagcc taggtggagc
ctgacaggac gcacgaccgg 8220ctagcccgtg acagacgatg ggtggctcct
gttgtccacc gcgtacaaat gtttgggcca 8280aagtcttgtc agccttgctt
gcgaacctaa ttcccaattt tgtcacttcg cacccccatt 8340gatcgagccc
taacccctgc ccatcaggca atccaattaa gctcgcattg tctgccttgt
8400ttagtttggc tcctgcccgt ttcggcgtcc acttgcacaa acacaaacaa
gcattatata 8460taaggctcgt ctctccctcc caaccacact cacttttttg
cccgtcttcc cttgctaaca 8520caaaagtcaa gaacacaaac aaccacccca
acccccttac acacaagaca tatctacagc 8580aatggccatg ggcaagggtg
gagacggtgg agcacaggct gtgtctggca ccgatgcctc 8640cctcgctgag
gtctcctctg tggacagcaa gtccgtccac gtggttctgt acggcaagcg
8700agtggatgtc accaagttcc agaaggctca ccctggaggt tcgaaggtgt
tccgaatctt 8760tcaggagcga gacgccacag aacagttcga gtcctaccac
tctcccaagg ccatcaagat 8820gatggaaggt atgctcaaaa agtcggagga
tgctcccgct tccgtgcctc ttccctctcg 8880atccactatg ggcaccgagt
tcaaggagat gatcgaacga cacaagagag ccggtctcta 8940cgacccttgt
cccttggacg agctgttcaa gctcaccatt gtccttgctc ctatctttgt
9000gggagcctat ctcgttcgat ccggtgtctc tcctcttgct ggagccctgt
cgatgggctt 9060cggattctac ctcgacggct ggcttgctca cgactacctg
catcacgcag tgttcaaggg 9120ctccgtcaac acactcgtca aggccaacaa
cgctatggga tacgccttgg gcttcctcca 9180gggttacgac gttgcttggt
ggcgagccag acacaacact catcacgtgt gcaccaacga 9240ggacggctcc
gatcccgaca tcaagacggc tcctctgctc atttacgtgc gagagaatcc
9300ctccattgcc aagcggctca acttctttca gcgatggcaa cagtactact
atgttcctac 9360tatggccatt ctggatctct actggcgact ggagtctatc
gcatacgtcg ctgtgcgact 9420gcccaagatg tggatgcagg ctgccgctct
tgccgctcac tacgcactcc tgtgttgggt 9480cttcgctgcc catctcaacc
tgattcctct catgatggtt gcacgaggtt tcgcgaccgg 9540aatcgtggtc
tttgccaccc actatggcga ggacattctc gaccgagagc acgtcgaagg
9600catgactctg gtcgagcaga ccgccaagac ctcccgaaac atcactggtg
gatggcttgt 9660caacgtgctc accggcttca tttctctgca gaccgagcat
catctgtttc ccatgatgcc 9720tactggaaac ctcatgacca ttcaacccga
ggttcgagac tttttcaaaa agcacggtct 9780cgagtaccga gaaggaaacc
tgtttcagtg cgtgcatcag aacatcaagg ctctcgcctt 9840cgagcacctg
cttcactaag cggccgcatt gatgattgga aacacacaca tgggttatat
9900ctaggtgaga gttagttgga cagttatata ttaaatcagc tatgccaacg
gtaacttcat 9960tcatgtcaac gaggaaccag tgactgcaag taatatagaa
tttgaccacc ttgccattct 10020cttgcactcc tttactatat ctcatttatt
tcttatatac aaatcacttc ttcttcccag 10080catcgagctc ggaaacctca
tgagcaataa catcgtggat ctcgtcaata gagggctttt 10140tggactcctt
gctgttggcc accttgtcct tgctgtctgg ctcattctgt ttcaacgcct 10200tttaat
10206726871DNAArtificial Sequenceplasmid pEXPGUS1-C 72aattcgtcct
tgaggactcg agtgacagtc tttcgccaaa gtcgagagga ggccagcacg 60ttggccttgt
caagagacca cacgggaaga ggggggttgt gctgaagggc caggaaggcg
120gccattcggg caattcgctc aacctcagga acggagtagg tctcggtgtc
ggaagcgacg 180ccagatccgt catcctcctt tcgctctcca aagtagatac
ctccgacgag ctctcggaca 240atgatgaagt cggtgccctc aacgtttcgg
atgggggaga gatcggcgag cttgggcgac 300agcagctggc agggtcgcag
gttggcgtac aggttcaggt cctttcgcag cttgaggaga 360ccctgctcgg
gtcgcacgtc ggttcgtccg tcgggagtgg tccatacggt gttggcagcg
420cctccgacag caccgagcat aatagagtca gcctttcggc agatgtcgag
agtagcgtcg 480gtgatgggct cgccctcctt ctcaatggca gctcctccaa
tgagtcggtc ctcaaacaca 540aactcggtgc cggaggcctc agcaacagac
ttgagcacct tgacggcctc ggcaatcacc 600tcggggccac agaagtcgcc
gccgagaaga acaatcttct tggagtcagt cttggtcttc 660ttagtttcgg
gttccattgt ggatgtgtgt ggttgtatgt gtgatgtggt gtgtggagtg
720aaaatctgtg gctggcaaac gctcttgtat atatacgcac ttttgcccgt
gctatgtgga 780agactaaacc tccgaagatt gtgactcagg tagtgcggta
tcggctaggg acccaaacct 840tgtcgatgcc gatagcgcta tcgaacgtac
cccagccggc cgggagtatg tcggagggga 900catacgagat cgtcaagggt
ttgtggccaa ctggtaaata aatgatgtcg agggagtttg 960gcgcccgttt
tttcgagccc cacacgtttc ggtgagtatg agcggcggca gattcgagcg
1020tttccggttt ccgcggctgg acgagagccc atgatggggg ctcccaccac
cagcaatcag 1080ggccctgatt acacacccac ctgtaatgtc atgctgttca
tcgatggtta atgctgctgt 1140gtgctgtgtg tgtgtgttgt ttggcgctca
ttgttgcgtt atgcagcgta caccacaata 1200ttggaagctt attagccttt
ctattttttc gtttgcaagg cttaacaaca ttgctgtgga 1260gagggatggg
gatatggagg ccgctggagg gagtcggaga ggcgttttgg agcggcttgg
1320cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa
tgtgccactt 1380ttcagtctag taacgcctta cctacgtcat tccatgcgtg
catgtttgcg ccttttttcc 1440cttgcccttg atcgccacac agtacagtgc
actgtacagt ggaggttttg ggggggtctt 1500agatgggagc taaaagcggc
ctagcggtac actagtggga ttgtatggag tggcatggag 1560cctaggtgga
gcctgacagg acgcacgacc ggctagcccg tgacagacga tgggtggctc
1620ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc
ttgcgaacct 1680aattcccaat tttgtcactt cgcaccccca ttgatcgagc
cctaacccct gcccatcagg 1740caatccaatt aagctcgcat tgtctgcctt
gtttagtttg gctcctgccc gtttcggcgt 1800ccacttgcac aaacacaaac
aagcattata tataaggctc gtctctccct cccaaccaca 1860ctcacttttt
tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa acaaccaccc
1920caaccccctt acacacaaga catatctaca gcaatggcca tggtacgtcc
tgtagaaacc 1980ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat
tcagtctgga tcgcgaaaac 2040tgtggaattg atcagcgttg gtgggaaagc
gcgttacaag aaagccgggc aattgctgtg 2100ccaggcagtt ttaacgatca
gttcgccgat gcagatattc gtaattatgc gggcaacgtc 2160tggtatcagc
gcgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt
2220ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt
gatggagcat 2280cagggcggct atacgccatt tgaagccgat gtcacgccgt
atgttattgc cgggaaaagt 2340gtacgtatca ccgtttgtgt gaacaacgaa
ctgaactggc agactatccc gccgggaatg 2400gtgattaccg acgaaaacgg
caagaaaaag cagtcttact tccatgattt ctttaactat 2460gccgggatcc
atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc
2520accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg
gcaggtggtg 2580gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc
aacaggtggt tgcaactgga 2640caaggcacta gcgggacttt gcaagtggtg
aatccgcacc tctggcaacc gggtgaaggt 2700tatctctatg aactgtgcgt
cacagccaaa agccagacag agtgtgatat ctacccgctt 2760cgcgtcggca
tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg
2820ttctacttta ctggctttgg tcgtcatgaa gatgcggact tacgtggcaa
aggattcgat 2880aacgtgctga tggtgcacga ccacgcatta atggactgga
ttggggccaa ctcctaccgt 2940acctcgcatt acccttacgc tgaagagatg
ctcgactggg cagatgaaca tggcatcgtg 3000gtgattgatg aaactgctgc
tgtcggcttt aacctctctt taggcattgg tttcgaagcg 3060ggcaacaagc
cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg
3120cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag
cgtggtgatg 3180tggagtattg ccaacgaacc ggatacccgt ccgcaagtgc
acgggaatat ttcgccactg 3240gcggaagcaa cgcgtaaact cgacccgacg
cgtccgatca cctgcgtcaa tgtaatgttc 3300tgcgacgctc acaccgatac
catcagcgat ctctttgatg tgctgtgcct gaaccgttat 3360tacggatggt
atgtccaaag cggcgatttg gaaacggcag agaaggtact ggaaaaagaa
3420cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
cggcgtggat 3480acgttagccg ggctgcactc aatgtacacc gacatgtgga
gtgaagagta tcagtgtgca 3540tggctggata tgtatcaccg cgtctttgat
cgcgtcagcg ccgtcgtcgg tgaacaggta 3600tggaatttcg ccgattttgc
gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa 3660gggatcttca
ctcgcgaccg caaaccgaag tcggcggctt ttctgctgca aaaacgctgg
3720actggcatga acttcggtga aaaaccgcag cagggaggca aacaatgatt
aattaactag 3780agcggccgcc accgcggccc gagattccgg cctcttcggc
cgccaagcga cccgggtgga 3840cgtctagagg tacctagcaa ttaacagata
gtttgccggt gataattctc ttaacctccc 3900acactccttt gacataacga
tttatgtaac gaaactgaaa tttgaccaga tattgtgtcc 3960gcggtggagc
tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
4020atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
acaacatacg 4080agccggaagc ataaagtgta aagcctgggg tgcctaatga
gtgagctaac tcacattaat 4140tgcgttgcgc tcactgcccg ctttccagtc
gggaaacctg tcgtgccagc tgcattaatg 4200aatcggccaa cgcgcgggga
gaggcggttt gcgtattggg cgctcttccg cttcctcgct 4260cactgactcg
ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
4320ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
gagcaaaagg 4380ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
gcgtttttcc ataggctccg 4440cccccctgac gagcatcaca aaaatcgacg
ctcaagtcag aggtggcgaa acccgacagg 4500actataaaga taccaggcgt
ttccccctgg aagctccctc gtgcgctctc ctgttccgac 4560cctgccgctt
accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
4620tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
tgggctgtgt 4680gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
ggtaactatc gtcttgagtc 4740caacccggta agacacgact tatcgccact
ggcagcagcc actggtaaca ggattagcag 4800agcgaggtat gtaggcggtg
ctacagagtt cttgaagtgg tggcctaact acggctacac 4860tagaaggaca
gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4920tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
ttgtttgcaa 4980gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
cctttgatct tttctacggg 5040gtctgacgct cagtggaacg aaaactcacg
ttaagggatt ttggtcatga gattatcaaa 5100aaggatcttc acctagatcc
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 5160atatgagtaa
acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
5220gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
taactacgat 5280acgggagggc ttaccatctg gccccagtgc tgcaatgata
ccgcgagacc cacgctcacc 5340ggctccagat ttatcagcaa taaaccagcc
agccggaagg gccgagcgca gaagtggtcc 5400tgcaacttta tccgcctcca
tccagtctat taattgttgc cgggaagcta gagtaagtag 5460ttcgccagtt
aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
5520ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
gagttacatg 5580atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
cctccgatcg ttgtcagaag 5640taagttggcc gcagtgttat cactcatggt
tatggcagca ctgcataatt ctcttactgt 5700catgccatcc gtaagatgct
tttctgtgac tggtgagtac tcaaccaagt cattctgaga 5760atagtgtatg
cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
5820acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
gaaaactctc 5880aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc 5940ttcagcatct tttactttca ccagcgtttc
tgggtgagca aaaacaggaa ggcaaaatgc 6000cgcaaaaaag ggaataaggg
cgacacggaa atgttgaata ctcatactct tcctttttca 6060atattattga
agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
6120ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
cacctgacgc 6180gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
acgcgcagcg tgaccgctac 6240acttgccagc gccctagcgc ccgctccttt
cgctttcttc ccttcctttc tcgccacgtt 6300cgccggcttt ccccgtcaag
ctctaaatcg ggggctccct ttagggttcc gatttagtgc 6360tttacggcac
ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
6420gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
atagtggact 6480cttgttccaa actggaacaa cactcaaccc tatctcggtc
tattcttttg atttataagg 6540gattttgccg atttcggcct attggttaaa
aaatgagctg atttaacaaa aatttaacgc 6600gaattttaac aaaatattaa
cgcttacaat ttccattcgc cattcaggct gcgcaactgt 6660tgggaagggc
gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
6720gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
ttgtaaaacg 6780acggccagtg aattgtaata cgactcacta tagggcgaat
tgggtaccgg gccccccctc 6840gaggtcgatg gtgtcgataa gcttgatatc g
6871735460DNAArtificial Sequenceplasmid pZGD5T-CP 73ggccgcattg
atgattggaa acacacacat gggttatatc taggtgagag ttagttggac 60agttatatat
taaatcagct atgccaacgg taacttcatt catgtcaacg aggaaccagt
120gactgcaagt aatatagaat ttgaccacct tgccattctc ttgcactcct
ttactatatc 180tcatttattt cttatataca aatcacttct tcttcccagc
atcgagctcg gaaacctcat 240gagcaataac atcgtggatc tcgtcaatag
agggcttttt ggactccttg ctgttggcca 300ccttgtcctt gctgtctggc
tcattctgtt tcaacgcctt ttaattaatc gagcttggcg 360taatcatggt
catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
420atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
ctaactcaca 480ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat 540taatgaatcg gccaacgcgc ggggagaggc
ggtttgcgta ttgggcgctc ttccgcttcc 600tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca 660aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
720aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
tttccatagg 780ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
gtcagaggtg gcgaaacccg 840acaggactat aaagatacca ggcgtttccc
cctggaagct ccctcgtgcg ctctcctgtt 900ccgaccctgc cgcttaccgg
atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 960tctcatagct
cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1020tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
ctatcgtctt 1080gagtccaacc cggtaagaca cgacttatcg ccactggcag
cagccactgg taacaggatt 1140agcagagcga ggtatgtagg cggtgctaca
gagttcttga agtggtggcc taactacggc 1200tacactagaa ggacagtatt
tggtatctgc gctctgctga agccagttac cttcggaaaa 1260agagttggta
gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1320tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
gatcttttct 1380acggggtctg acgctcagtg gaacgaaaac tcacgttaag
ggattttggt catgagatta 1440tcaaaaagga tcttcaccta gatcctttta
aattaaaaat gaagttttaa atcaatctaa 1500agtatatatg agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc 1560tcagcgatct
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
1620acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
agacccacgc 1680tcaccggctc cagatttatc agcaataaac cagccagccg
gaagggccga gcgcagaagt 1740ggtcctgcaa ctttatccgc ctccatccag
tctattaatt gttgccggga agctagagta 1800agtagttcgc cagttaatag
tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1860tcacgctcgt
cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
1920acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc 1980agaagtaagt tggccgcagt gttatcactc atggttatgg
cagcactgca taattctctt 2040actgtcatgc catccgtaag atgcttttct
gtgactggtg agtactcaac caagtcattc 2100tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaatacg ggataatacc 2160gcgccacata
gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
2220ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac 2280tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa 2340aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt 2400tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa 2460tgtatttaga
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
2520gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
cagcgtgacc 2580gctacacttg ccagcgccct agcgcccgct cctttcgctt
tcttcccttc ctttctcgcc 2640acgttcgccg gctttccccg tcaagctcta
aatcgggggc tccctttagg gttccgattt 2700agtgctttac ggcacctcga
ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 2760ccatcgccct
gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
2820ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
ttttgattta 2880taagggattt tgccgatttc ggcctattgg ttaaaaaatg
agctgattta acaaaaattt 2940aacgcgaatt ttaacaaaat attaacgctt
acaatttcca ttcgccattc aggctgcgca 3000actgttggga agggcgatcg
gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 3060gatgtgctgc
aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
3120aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
accgggcccc 3180ccctcgaggt cgacggtatc gatggaagcc ggtagaaccg
ggctgcttgt gcttggagat 3240ggaagccggt agaaccgggc tgcttggggg
gatttggggc cgctgggctc caaagagggg 3300taggcatttc gttggggtta
cgtaattgcg gcatttgggt cctgcgcgca tgtcccattg 3360gtcagaatta
gtccggatag gagacttatc agccaatcac agcgccggat ccacctgtag
3420gttgggttgg gtgggagcac ccctccacag agtagagtca aacagcagca
gcaacatgat 3480agttgggggt gtgcgtgtta aaggaaaaaa aagaagcttg
ggttatattc ccgctctatt 3540tagaggttgc gggatagacg ccgacggagg
gcaatggcgc tatggaacct tgcggatatc 3600catacgccgc ggcggactgc
gtccgaacca gctccagcag cgttttttcc gggccattga 3660gccgactgcg
accccgccaa cgtgtcttgg cccacgcact catgtcatgt tggtgttggg
3720aggccacttt ttaagtagca caaggcacct agctcgcagc aaggtgtccg
aaccaaagaa 3780gcggctgcag tggtgcaaac ggggcggaaa cggcgggaaa
aagccacggg ggcacgaatt 3840gaggcacgcc ctcgaatttg agacgagtca
cggccccatt cgcccgcgca atggctcgcc 3900aacgcccggt cttttgcacc
acatcaggtt accccaagcc aaacctttgt gttaaaaagc 3960ttaacatatt
ataccgaacg taggtttggg cgggcttgct ccgtctgtcc aaggcaacat
4020ttatataagg gtctgcatcg ccggctcaat tgaatctttt ttcttcttct
cttctctata 4080ttcattcttg aattaaacac acatcaacca tgggaacgga
ccaaggaaaa accttcacct 4140gggaagagct ggcggcccat aacaccaagg
acgacctact cttggccatc cgcggcaggg 4200tgtacgatgt cacaaagttc
ttgagccgcc atcctggtgg agtggacact ctcctgctcg 4260gagctggccg
agatgttact ccggtctttg agatgtatca cgcgtttggg gctgcagatg
4320ccattatgaa gaagtactat gtcggtacac tggtctcgaa tgagctgccc
atcttcccgg 4380agccaacggt gttccacaaa accatcaaga cgagagtcga
gggctacttt acggatcgga 4440acattgatcc caagaataga ccagagatct
ggggacgata cgctcttatc tttggatcct 4500tgatcgcttc ctactacgcg
cagctctttg tgcctttcgt tgtcgaacgc acatggcttc 4560aggtggtgtt
tgcaatcatc atgggatttg cgtgcgcaca agtcggactc aaccctcttc
4620atgatgcgtc tcacttttca gtgacccaca accccactgt ctggaagatt
ctgggagcca 4680cgcacgactt tttcaacgga gcatcgtacc tggtgtggat
gtaccaacat atgctcggcc 4740atcaccccta caccaacatt gctggagcag
atcccgacgt gtcgacgtct gagcccgatg 4800ttcgtcgtat caagcccaac
caaaagtggt ttgtcaacca catcaaccag cacatgtttg 4860ttcctttcct
gtacggactg ctggcgttca aggtgcgcat tcaggacatc aacattttgt
4920actttgtcaa gaccaatgac gctattcgtg tcaatcccat ctcgacatgg
cacactgtga 4980tgttctgggg cggcaaggct ttctttgtct ggtatcgcct
gattgttccc ctgcagtatc 5040tgcccctggg caaggtgctg ctcttgttca
cggtcgcgga catggtgtcg tcttactggc 5100tggcgctgac cttccaggcg
aaccacgttg ttgaggaagt tcagtggccg ttgcctgacg 5160agaacgggat
catccaaaag gactgggcag ctatgcaggt cgagactacg caggattacg
5220cacacgattc gcacctctgg accagcatca ctggcagctt gaactaccag
gctgtgcacc 5280atctgttccc caacgtgtcg cagcaccatt atcccgatat
tctggccatc atcaagaaca 5340cctgcagcga gtacaaggtt ccataccttg
tcaaggatac gttttggcaa gcatttgctt 5400cacatttgga gcacttgcgt
gttcttggac tccgtcccaa ggaagagtag gcagctaagc 5460748630DNAArtificial
Sequenceplasmid pYZDE2-S 74ggtggagctc cagcttttgt tccctttagt
gagggttaat ttcgagcttg gcgtaatcat 60ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac aacgtacgag 120ccggaagcat aaagtgtaaa
gcctggggtg cctaatgagt gagctaactc acattaattg 180cgttgcgctc
actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
240tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
tcctcgctca 300ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
atcagctcac tcaaaggcgg 360taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc 420agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc 480cccctgacga
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
540tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc 600tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg ctttctcata 660gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc 720acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca 780acccggtaag
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
840cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
ggctacacta 900gaaggacagt atttggtatc tgcgctctgc tgaagccagt
taccttcgga aaaagagttg 960gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc 1020agcagattac gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt 1080ctgacgctca
gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
1140ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
taaagtatat 1200atgagtaaac ttggtctgac agttaccaat gcttaatcag
tgaggcacct atctcagcga 1260tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac 1320gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg 1380ctccagattt
atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
1440caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
gtaagtagtt 1500cgccagttaa tagtttgcgc aacgttgttg ccattgctac
aggcatcgtg gtgtcacgct 1560cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat 1620cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 1680agttggccgc
agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
1740tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
ttctgagaat 1800agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
acgggataat accgcgccac 1860atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa 1920ggatcttacc gctgttgaga
tccagttcga tgtaacccac tcgtgcaccc aactgatctt 1980cagcatcttt
tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
2040caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
ctttttcaat 2100attattgaag catttatcag ggttattgtc tcatgagcgg
atacatattt gaatgtattt 2160agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgacgcgc 2220cctgtagcgg cgcattaagc
gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2280ttgccagcgc
cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2340ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
tttagtgctt 2400tacggcacct cgaccccaaa aaacttgatt agggtgatgg
ttcacgtagt gggccatcgc 2460cctgatagac ggtttttcgc cctttgacgt
tggagtccac gttctttaat agtggactct 2520tgttccaaac tggaacaaca
ctcaacccta tctcggtcta ttcttttgat ttataaggga 2580ttttgccgat
ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
2640attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc
gcaactgttg 2700ggaagggcga tcggtgcggg cctcttcgct attacgccag
ctggcgaaag ggggatgtgc 2760tgcaaggcga ttaagttggg taacgccagg
gttttcccag tcacgacgtt gtaaaacgac 2820ggccagtgaa ttgtaatacg
actcactata gggcgaattg ggtaccgggc cccccctcga 2880ggtcgatggt
gtcgataagc ttgatatcga attcatgtca cacaaaccga tcttcgcctc
2940aaggaaacct aattctacat ccgagagact gccgagatcc agtctacact
gattaatttt 3000cgggccaata atttaaaaaa atcgtgttat ataatattat
atgtattata tatatacatc 3060atgatgatac tgacagtcat gtcccattgc
taaatagaca gactccatct gccgcctcca 3120actgatgttc tcaatattta
aggggtcatc tcgcattgtt taataataaa cagactccat 3180ctaccgcctc
caaatgatgt tctcaaaata tattgtatga acttattttt attacttagt
3240attattagac aacttacttg ctttatgaaa aacacttcct atttaggaaa
caatttataa 3300tggcagttcg ttcatttaac aatttatgta gaataaatgt
tataaatgcg tatgggaaat 3360cttaaatatg gatagcataa atgatatctg
cattgcctaa ttcgaaatca acagcaacga 3420aaaaaatccc ttgtacaaca
taaatagtca tcgagaaata tcaactatca aagaacagct 3480attcacacgt
tactattgag attattattg gacgagaatc acacactcaa ctgtctttct
3540ctcttctaga aatacaggta caagtatgta ctattctcat tgttcatact
tctagtcatt 3600tcatcccaca tattccttgg atttctctcc aatgaatgac
attctatctt gcaaattcaa 3660caattataat aagatatacc aaagtagcgg
tatagtggca atcaaaaagc ttctctggtg 3720tgcttctcgt atttattttt
attctaatga tccattaaag gtatatattt atttcttgtt 3780atataatcct
tttgtttatt acatgggctg gatacataaa ggtattttga tttaattttt
3840tgcttaaatt caatcccccc tcgttcagtg tcaactgtaa tggtaggaaa
ttaccatact 3900tttgaagaag caaaaaaaat gaaagaaaaa aaaaatcgta
tttccaggtt agacgttccg 3960cagaatctag aatgcggtat gcggtacatt
gttcttcgaa cgtaaaagtt gcgctccctg 4020agatattgta catttttgct
tttacaagta caagtacatc gtacaactat gtactactgt 4080tgatgcatcc
acaacagttt gttttgtttt tttttgtttt ttttttttct aatgattcat
4140taccgctatg tatacctact tgtacttgta gtaagccggg ttattggcgt
tcaattaatc 4200atagacttat gaatctgcac ggtgtgcgct gcgagttact
tttagcttat gcatgctact 4260tgggtgtaat attgggatct gttcggaaat
caacggatgc tcaaccgatt tcgacagtaa 4320taatttgaat cgaatcggag
cctaaaatga acccgagtat atctcataaa attctcggtg 4380agaggtctgt
gactgtcagt acaaggtgcc ttcattatgc cctcaacctt accatacctc
4440actgaatgta gtgtacctct aaaaatgaaa tacagtgcca aaagccaagg
cactgagctc 4500gtctaacgga cttgatatac aaccaattaa aacaaatgaa
aagaaataca gttctttgta 4560tcatttgtaa caattaccct gtacaaacta
aggtattgaa atcccacaat attcccaaag 4620tccacccctt tccaaattgt
catgcctaca actcatatac caagcactaa cctaccaaac 4680accactaaaa
ccccacaaaa tatatcttac cgaatataca gtaacaagct accaccacac
4740tcgttgggtg cagtcgccag cttaaagata tctatccaca tcagccacaa
ctcccttcct 4800ttaataaacc gactacaccc ttggctattg aggttatgag
tgaatatact gtagacaaga 4860cactttcaag aagactgttt ccaaaacgta
ccactgtcct ccactacaaa cacacccaat 4920ctgcttcttc tagtcaaggt
tgctacaccg gtaaattata aatcatcatt tcattagcag 4980ggcagggccc
tttttataga gtcttataca ctagcggacc ctgccggtag accaacccgc
5040aggcgcgtca gtttgctcct tccatcaatg cgtcgtagaa acgacttact
ccttcttgag 5100cagctccttg accttgttgg caacaagtct ccgacctcgg
aggtggagga agagcctccg 5160atatcggcgg tagtgatacc agcctcgacg
gactccttga cggcagcctc aacagcgtca 5220ccggcgggct tcatgttaag
agagaacttg agcatcatgg cggcagacag aatggtggca 5280atggggttga
ccttctgctt gccgagatcg ggggcagatc cgtgacaggg ctcgtacaga
5340ccgaacgcct cgttggtgtc gggcagagaa gccagagagg cggagggcag
cagacccaga 5400gaaccgggga tgacggaggc ctcgtcggag atgatatcgc
caaacatgtt ggtggtgatg 5460atgataccat tcatcttgga gggctgcttg
atgaggatca tggcggccga gtcgatcagc 5520tggtggttga gctcgagctg
ggggaattcg tccttgagga ctcgagtgac agtctttcgc 5580caaagtcgag
aggaggccag cacgttggcc ttgtcaagag accacacggg aagagggggg
5640ttgtgctgaa gggccaggaa ggcggccatt cgggcaattc gctcaacctc
aggaacggag 5700taggtctcgg tgtcggaagc gacgccagat ccgtcatcct
cctttcgctc tccaaagtag 5760atacctccga cgagctctcg gacaatgatg
aagtcggtgc cctcaacgtt tcggatgggg 5820gagagatcgg cgagcttggg
cgacagcagc tggcagggtc gcaggttggc gtacaggttc 5880aggtcctttc
gcagcttgag gagaccctgc tcgggtcgca cgtcggttcg tccgtcggga
5940gtggtccata cggtgttggc agcgcctccg acagcaccga gcataataga
gtcagccttt 6000cggcagatgt cgagagtagc gtcggtgatg ggctcgccct
ccttctcaat ggcagctcct 6060ccaatgagtc ggtcctcaaa cacaaactcg
gtgccggagg cctcagcaac agacttgagc 6120accttgacgg cctcggcaat
cacctcgggg ccacagaagt cgccgccgag aagaacaatc 6180ttcttggagt
cagtcttggt cttcttagtt tcgggttcca ttgtggatgt gtgtggttgt
6240atgtgtgatg tggtgtgtgg agtgaaaatc tgtggctggc aaacgctctt
gtatatatac 6300gcacttttgc ccgtgctatg tggaagacta aacctccgaa
gattgtgact caggtagtgc 6360ggtatcggct agggacccaa accttgtcga
tgccgatagc gctatcgaac gtaccccagc 6420cggccgggag tatgtcggag
gggacatacg agatcgtcaa gggtttgtgg ccaactggta 6480aatttaaatg
atgtcgacgc agtaggatgt cctgcacggg tctttttgtg gggtgtggag
6540aaaggggtgc ttggagatgg aagccggtag aaccgggctg cttgtgcttg
gagatggaag 6600ccggtagaac cgggctgctt ggggggattt ggggccgctg
ggctccaaag aggggtaggc 6660atttcgttgg ggttacgtaa ttgcggcatt
tgggtcctgc gcgcatgtcc cattggtcag 6720aattagtccg gataggagac
ttatcagcca atcacagcgc cggatccacc tgtaggttgg 6780gttgggtggg
agcacccctc cacagagtag agtcaaacag cagcagcaac atgatagttg
6840ggggtgtgcg tgttaaagga aaaaaaagaa gcttgggtta tattcccgct
ctatttagag 6900gttgcgggat agacgccgac ggagggcaat ggcgccatgg
aaccttgcgg atatcgatac 6960gccgcggcgg actgcgtccg aaccagctcc
agcagcgttt tttccgggcc attgagccga 7020ctgcgacccc gccaacgtgt
cttggcccac gcactcatgt catgttggtg ttgggaggcc 7080actttttaag
tagcacaagg cacctagctc gcagcaaggt gtccgaacca aagaagcggc
7140tgcagtggtg caaacggggc ggaaacggcg ggaaaaagcc acgggggcac
gaattgaggc 7200acgccctcga atttgagacg agtcacggcc ccattcgccc
gcgcaatggc tcgccaacgc 7260ccggtctttt gcaccacatc aggttacccc
aagccaaacc tttgtgttaa aaagcttaac 7320atattatacc gaacgtaggt
ttgggcgggc ttgctccgtc tgtccaaggc aacatttata 7380taagggtctg
catcgccggc tcaattgaat cttttttctt cttctcttct ctatattcat
7440tcttgaatta aacacacatc aatccatggc aaacagcagc gtgtgggatg
atgtggtggg 7500ccgcgtggag accggcgtgg accagtggat ggatggcgcc
aagccgtacg cactcaccga 7560tgggctcccg atgatggacg tgtccaccat
gctggcattc gaggtgggat acatggccat 7620gctgctcttc ggcatcccga
tcatgaagca gatggagaag ccttttgagc tcaagaccat 7680caagctcttg
cacaacttgt ttctcttcgg actttccttg tacatgtgcg tggagaccat
7740ccgccaggct atcctcggag gctacaaagt gtttggaaac gacatggaga
agggcaacga 7800gtctcatgct cagggcatgt ctcgcatcgt gtacgtgttc
tacgtgtcca aggcatacga 7860gttcttggat accgccatca tgatcctttg
caagaagttc aaccaggttt ccttcttgca 7920tgtgtaccac catgccactc
atttttgcca tctggtgggc tatccgccaa gtacgctcca 7980ggaggtgatg
cgtacttttt cagtgatcct caactctttc gtgcacaccg tcatgtacgg
8040catactactt cttctcctcc caagggttcg ggttcgtgaa gccaatcaag
ccgtacatca 8100ccacccttca gatgacccag ttcatggcaa tgcttgtgca
gtccttgtac gactacctct 8160tcccatgcga ctacccacag gctcttgtgc
agctccttgg agtgtacatg atcaccttgc 8220ttgccctctt cggcaacttt
tttgtgcaga gctatcttaa aaagccaaaa aagagcaaga 8280ccaactaaaa
ctgcctgcat gatatgccgc tcgccggcgt tcgaattgac tcagaaagcg
8340agttaaggcg acacgcaaac tctatatttt ttcaaacgtg ttgccgtcac
tcattcgcca 8400tctgtttact acgtgtctgt tcaatgagca tgttcttgaa
tctaaagaat ctcgaatgtt 8460ttttaaaaaa agaattcgat atcaagctta
cgcgtcgacc cgggtggacg tctagaggta 8520cctagcaatt aacagatagt
ttgccggtga taattctctt aacctcccac actcctttga 8580cataacgatt
tatgtaacga aactgaaatt tgaccagata ttgtgtccgc 8630756473DNAArtificial
Sequenceplasmid pY5-22 75ggtggagctc cagcttttgt tccctttagt
gagggttaat ttcgagcttg gcgtaatcat 60ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac aacgtacgag 120ccggaagcat aaagtgtaaa
gcctggggtg cctaatgagt gagctaactc acattaattg 180cgttgcgctc
actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
240tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
tcctcgctca 300ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
atcagctcac tcaaaggcgg 360taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc 420agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc 480cccctgacga
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
540tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc 600tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg ctttctcata 660gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc 720acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca 780acccggtaag
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
840cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
ggctacacta 900gaaggacagt atttggtatc tgcgctctgc tgaagccagt
taccttcgga aaaagagttg 960gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc 1020agcagattac gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt 1080ctgacgctca
gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
1140ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
taaagtatat 1200atgagtaaac ttggtctgac agttaccaat gcttaatcag
tgaggcacct atctcagcga 1260tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac 1320gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg 1380ctccagattt
atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
1440caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
gtaagtagtt 1500cgccagttaa tagtttgcgc aacgttgttg ccattgctac
aggcatcgtg gtgtcacgct 1560cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat 1620cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 1680agttggccgc
agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
1740tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
ttctgagaat 1800agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
acgggataat accgcgccac 1860atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa 1920ggatcttacc gctgttgaga
tccagttcga tgtaacccac tcgtgcaccc aactgatctt 1980cagcatcttt
tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
2040caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
ctttttcaat 2100attattgaag catttatcag ggttattgtc tcatgagcgg
atacatattt gaatgtattt 2160agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgacgcgc 2220cctgtagcgg cgcattaagc
gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2280ttgccagcgc
cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2340ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
tttagtgctt 2400tacggcacct cgaccccaaa aaacttgatt agggtgatgg
ttcacgtagt gggccatcgc 2460cctgatagac ggtttttcgc cctttgacgt
tggagtccac gttctttaat agtggactct 2520tgttccaaac tggaacaaca
ctcaacccta tctcggtcta ttcttttgat ttataaggga 2580ttttgccgat
ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
2640attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc
gcaactgttg 2700ggaagggcga tcggtgcggg cctcttcgct attacgccag
ctggcgaaag ggggatgtgc 2760tgcaaggcga ttaagttggg taacgccagg
gttttcccag tcacgacgtt gtaaaacgac 2820ggccagtgaa ttgtaatacg
actcactata gggcgaattg ggtaccgggc cccccctcga 2880ggtcgatggt
gtcgataagc ttgatatcga attcatgtca cacaaaccga tcttcgcctc
2940aaggaaacct aattctacat ccgagagact gccgagatcc agtctacact
gattaatttt 3000cgggccaata atttaaaaaa atcgtgttat ataatattat
atgtattata tatatacatc 3060atgatgatac tgacagtcat gtcccattgc
taaatagaca gactccatct gccgcctcca 3120actgatgttc tcaatattta
aggggtcatc tcgcattgtt taataataaa cagactccat 3180ctaccgcctc
caaatgatgt tctcaaaata tattgtatga acttattttt attacttagt
3240attattagac aacttacttg ctttatgaaa aacacttcct atttaggaaa
caatttataa 3300tggcagttcg ttcatttaac aatttatgta gaataaatgt
tataaatgcg tatgggaaat 3360cttaaatatg gatagcataa atgatatctg
cattgcctaa ttcgaaatca acagcaacga 3420aaaaaatccc ttgtacaaca
taaatagtca tcgagaaata tcaactatca aagaacagct 3480attcacacgt
tactattgag attattattg gacgagaatc acacactcaa ctgtctttct
3540ctcttctaga aatacaggta caagtatgta ctattctcat tgttcatact
tctagtcatt 3600tcatcccaca tattccttgg atttctctcc aatgaatgac
attctatctt gcaaattcaa 3660caattataat aagatatacc aaagtagcgg
tatagtggca atcaaaaagc ttctctggtg 3720tgcttctcgt atttattttt
attctaatga tccattaaag gtatatattt atttcttgtt 3780atataatcct
tttgtttatt acatgggctg gatacataaa ggtattttga tttaattttt
3840tgcttaaatt caatcccccc tcgttcagtg tcaactgtaa tggtaggaaa
ttaccatact 3900tttgaagaag caaaaaaaat gaaagaaaaa aaaaatcgta
tttccaggtt agacgttccg 3960cagaatctag aatgcggtat gcggtacatt
gttcttcgaa cgtaaaagtt gcgctccctg 4020agatattgta catttttgct
tttacaagta caagtacatc gtacaactat gtactactgt 4080tgatgcatcc
acaacagttt gttttgtttt tttttgtttt ttttttttct aatgattcat
4140taccgctatg tatacctact tgtacttgta gtaagccggg ttattggcgt
tcaattaatc 4200atagacttat gaatctgcac ggtgtgcgct gcgagttact
tttagcttat gcatgctact 4260tgggtgtaat attgggatct gttcggaaat
caacggatgc tcaaccgatt tcgacagtaa 4320ttaattaagt catacacaag
tcagctttct tcgagcctca tataagtata agtagttcaa 4380cgtattagca
ctgtacccag catctccgta tcgagaaaca caacaacatg ccccattgga
4440cagatcatgc ggatacacag gttgtgcagt atcatacata ctcgatcaga
caggtcgtct 4500gaccatcata caagctgaac aagcgctcca tacttgcacg
ctctctatat acacagttaa 4560attacatatc catagtctaa cctctaacag
ttaatcttct ggtaagcctc ccagccagcc 4620ttctggtatc gcttggcctc
ctcaatagga tctcggttct ggccgtacag acctcggccg 4680acaattatga
tatccgttcc ggtagacatg acatcctcaa cagttcggta ctgctgtccg
4740agagcgtctc ccttgtcgtc aagacccacc ccgggggtca gaataagcca
gtcctcagag 4800tcgcccttag gtcggttctg ggcaatgaag ccaaccacaa
actcggggtc ggatcgggca 4860agctcaatgg tctgcttgga gtactcgcca
gtggccagag agcccttgca agacagctcg 4920gccagcatga gcagacctct
ggccagcttc tcgttgggag aggggactag gaactccttg 4980tactgggagt
tctcgtagtc agagacgtcc tccttcttct gttcagagac agtttcctcg
5040gcaccagctc gcaggccagc aatgattccg gttccgggta caccgtgggc
gttggtgata 5100tcggaccact cggcgattcg gtgacaccgg tactggtgct
tgacagtgtt gccaatatct 5160gcgaactttc tgtcctcgaa caggaagaaa
ccgtgcttaa gagcaagttc cttgaggggg 5220agcacagtgc cggcgtaggt
gaagtcgtca atgatgtcga tatgggtttt gatcatgcac 5280acataaggtc
cgaccttatc ggcaagctca atgagctcct tggtggtggt aacatccaga
5340gaagcacaca ggttggtttt cttggctgcc acgagcttga gcactcgagc
ggcaaaggcg 5400gacttgtgga cgttagctcg agcttcgtag gagggcattt
tggtggtgaa gaggagactg 5460aaataaattt agtctgcaga actttttatc
ggaaccttat ctggggcagt gaagtatatg 5520ttatggtaat agttacgagt
tagttgaact tatagataga ctggactata cggctatcgg 5580tccaaattag
aaagaacgtc aatggctctc tgggcgtcgc ctttgccgac aaaaatgtga
5640tcatgatgaa agccagcaat gacgttgcag ctgatattgt tgtcggccaa
ccgcgccgaa 5700aacgcagctg tcagacccac agcctccaac gaagaatgta
tcgtcaaagt gatccaagca 5760cactcatagt tggagtcgta ctccaaaggc
ggcaatgacg agtcagacag atactcgtcg 5820actcaggcga cgacggaatt
cctgcagccc atctgcagaa ttcaggagag accgggttgg 5880cggcgtattt
gtgtcccaaa aaacagcccc aattgcccca attgacccca aattgaccca
5940gtagcgggcc caaccccggc gagagccccc ttcaccccac atatcaaacc
tcccccggtt 6000cccacacttg ccgttaaggg cgtagggtac tgcagtctgg
aatctacgct tgttcagact 6060ttgtactagt ttctttgtct ggccatccgg
gtaacccatg ccggacgcaa aatagactac 6120tgaaaatttt tttgctttgt
ggttgggact ttagccaagg gtataaaaga ccaccgtccc 6180cgaattacct
ttcctcttct tttctctctc tccttgtcaa ctcacacccg aaatcgttaa
6240gcatttcctt ctgagtataa gaatcattca ccatggatcc actagttcta
gagcggccgc 6300caccgcggcc cgagattccg gcctcttcgg ccgccaagcg
acccgggtgg acgtctagag 6360gtacctagca attaacagat agtttgccgg
tgataattct cttaacctcc cacactcctt 6420tgacataacg atttatgtaa
cgaaactgaa atttgaccag atattgtgtc cgc 647376427PRTPavlova salina
76Met Gly Arg Gly Gly Asp Ser Ser Gly Gln Ala His Pro Ala Ala Glu1
5 10 15Leu Ala Val Pro Ser Asp Arg Ala Glu Val Ser Asn Ala Asp Ser
Lys 20 25 30Ala Leu His Ile Val Leu Tyr Gly Lys Arg Val Asp Val Thr
Lys Phe 35 40 45Gln Arg Thr His Pro Gly Gly Ser Lys Val Phe Arg Ile
Phe Gln Asp 50 55 60Arg Asp Ala Thr Glu Gln Phe Glu Ser Tyr His Ser
Lys Arg Ala Ile65 70 75 80Lys Met Met Glu Gly Met Leu Lys Lys Ser
Glu Asp Ala Pro Ala Asp 85 90 95Thr Pro Leu Pro Ser Gln Ser Pro Met
Gly Lys Asp Phe Lys Ala Met 100 105 110Ile Glu Arg His Val Ala Ala
Gly Tyr Tyr Asp Pro Cys Pro Leu Asp 115 120 125Glu Leu Phe Lys Leu
Ser Leu Val Leu Leu Pro Thr Phe Ala Gly Met 130 135 140Tyr Met Leu
Lys Ala Gly Val Gly Ser Pro Leu Cys Gly Ala Leu Met145 150 155
160Val Ser Phe Gly Trp Tyr Leu Asp Gly Trp Leu Ala His Asp Tyr Leu
165 170 175His His Ser Val Phe Lys Gly Ser Val Ala Arg Thr Val Gly
Trp Asn 180 185 190Asn Ala Ala Gly Tyr Phe Leu Gly Phe Val Gln Gly
Tyr Ala Val Glu 195 200 205Trp Trp Arg Ala Arg His Asn Thr His His
Val Cys Thr Asn Glu Asp 210 215 220Gly Ser Asp Pro Asp Ile Lys Thr
Ala Pro Leu Leu Ile Tyr Val Arg225 230 235 240Asn Lys Pro Ser Ile
Ala Lys Arg Leu Asn Ala Phe Gln Arg Tyr Gln 245 250 255Gln Tyr Tyr
Tyr Val Pro Val Met Ala Ile Leu Asp Leu Tyr Trp Arg 260 265 270Leu
Glu Ser Ile Ala Tyr Val Ala Met Arg Leu Pro Lys Met Leu Pro 275
280 285Gln Ala Leu Ala Leu Val Ala His Tyr Ala Ile Val Ala Trp Val
Phe 290 295 300Ala Gly Asn Tyr His Leu Leu Pro Leu Val Thr Val Leu
Arg Gly Phe305 310 315 320Gly Thr Gly Ile Thr Val Phe Ala Thr His
Tyr Gly Glu Asp Ile Leu 325 330 335Asp Ala Asp Gln Val Arg His Met
Thr Leu Val Glu Gln Thr Ala Leu 340 345 350Thr Ser Arg Asn Ile Ser
Gly Gly Trp Leu Val Asn Val Leu Thr Gly 355 360 365Phe Ile Ser Leu
Gln Thr Glu His His Leu Phe Pro Met Met Pro Thr 370 375 380Gly Asn
Leu Met Thr Ile Gln Pro Glu Val Arg Ala Phe Phe Lys Lys385 390 395
400His Gly Leu Glu Tyr Arg Glu Gly Asn Leu Ile Glu Cys Val Arg Gln
405 410 415Asn Ile Arg Ala Leu Ala Phe Glu His Leu Leu 420
42577421PRTEuglena gracilis 77Met Lys Ser Lys Arg Gln Ala Leu Pro
Leu Thr Ile Asp Gly Thr Thr1 5 10 15Tyr Asp Val Ser Ala Trp Val Asn
Phe His Pro Gly Gly Ala Glu Ile 20 25 30Ile Glu Asn Tyr Gln Gly Arg
Asp Ala Thr Asp Ala Phe Met Val Met 35 40 45His Ser Gln Glu Ala Phe
Asp Lys Leu Lys Arg Met Pro Lys Ile Asn 50 55 60Pro Ser Ser Glu Leu
Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu65 70 75 80Asp Phe Arg
Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp 85 90 95Ala Ser
Pro Leu Trp Tyr Ser Tyr Lys Ile Ser Thr Thr Leu Gly Leu 100 105
110Gly Val Leu Gly Tyr Phe Leu Met Val Gln Tyr Gln Met Tyr Phe Ile
115 120 125Gly Ala Val Leu Leu Gly Met His Tyr Gln Gln Met Gly Trp
Leu Ser 130 135 140His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg
Asn Trp Asn Asn145 150 155 160Leu Val Gly Leu Val Phe Gly Asn Gly
Leu Gln Gly Phe Ser Val Thr 165 170 175Trp Trp Lys Asp Arg His Asn
Ala His His Ser Ala Thr Asn Val Gln 180 185 190Gly His Asp Pro Asp
Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu 195 200 205Asp Asp Val
Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe 210 215 220Gln
Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp225 230
235 240Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp
Asn 245 250 255Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly
Leu Ala Leu 260 265 270His Trp Thr Leu Lys Thr Leu Phe His Leu Phe
Phe Met Pro Ser Ile 275 280 285Leu Thr Ser Leu Leu Val Phe Phe Val
Ser Glu Leu Val Gly Gly Phe 290 295 300Gly Ile Ala Ile Val Val Phe
Met Asn His Tyr Pro Leu Glu Lys Ile305 310 315 320Gly Asp Ser Val
Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His 325 330 335Glu Thr
Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly 340 345
350Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg
355 360 365His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys
Gln Lys 370 375 380His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu
Gly Leu Val Ile385 390 395 400Leu Leu Arg Tyr Leu Ala Val Phe Ala
Arg Met Ala Glu Lys Gln Pro 405 410 415Ala Gly Lys Ala Leu
420788953DNAArtificial Sequenceplasmid pY5-30 78ggtggagctc
cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat 60ggtcatagct
gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
120ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
acattaattg 180cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
gtgccagctg cattaatgaa 240tcggccaacg cgcggggaga ggcggtttgc
gtattgggcg ctcttccgct tcctcgctca 300ctgactcgct gcgctcggtc
gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 360taatacggtt
atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
420agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
aggctccgcc 480cccctgacga gcatcacaaa aatcgacgct caagtcagag
gtggcgaaac ccgacaggac 540tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc 600tgccgcttac cggatacctg
tccgcctttc tcccttcggg aagcgtggcg ctttctcata 660gctcacgctg
taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
720acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
cttgagtcca 780acccggtaag acacgactta tcgccactgg cagcagccac
tggtaacagg attagcagag 840cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta 900gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg 960gtagctcttg
atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
1020agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
tctacggggt 1080ctgacgctca gtggaacgaa aactcacgtt aagggatttt
ggtcatgaga ttatcaaaaa 1140ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat 1200atgagtaaac ttggtctgac
agttaccaat gcttaatcag tgaggcacct atctcagcga 1260tctgtctatt
tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
1320gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
cgctcaccgg 1380ctccagattt atcagcaata aaccagccag ccggaagggc
cgagcgcaga agtggtcctg 1440caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt 1500cgccagttaa tagtttgcgc
aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 1560cgtcgtttgg
tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
1620cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
gtcagaagta 1680agttggccgc agtgttatca ctcatggtta tggcagcact
gcataattct cttactgtca 1740tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat 1800agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaat acgggataat accgcgccac 1860atagcagaac
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
1920ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
aactgatctt 1980cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
aacaggaagg caaaatgccg 2040caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat 2100attattgaag catttatcag
ggttattgtc tcatgagcgg atacatattt gaatgtattt 2160agaaaaataa
acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
2220cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
accgctacac 2280ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
ttcctttctc gccacgttcg 2340ccggctttcc ccgtcaagct ctaaatcggg
ggctcccttt agggttccga tttagtgctt 2400tacggcacct cgaccccaaa
aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 2460cctgatagac
ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
2520tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
ttataaggga 2580ttttgccgat ttcggcctat tggttaaaaa atgagctgat
ttaacaaaaa tttaacgcga 2640attttaacaa aatattaacg cttacaattt
ccattcgcca ttcaggctgc gcaactgttg 2700ggaagggcga tcggtgcggg
cctcttcgct attacgccag ctggcgaaag ggggatgtgc 2760tgcaaggcga
ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
2820ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc
cccccctcga 2880ggtcgatggt gtcgataagc ttgatatcga attcatgtca
cacaaaccga tcttcgcctc 2940aaggaaacct aattctacat ccgagagact
gccgagatcc agtctacact gattaatttt 3000cgggccaata atttaaaaaa
atcgtgttat ataatattat atgtattata tatatacatc 3060atgatgatac
tgacagtcat gtcccattgc taaatagaca gactccatct gccgcctcca
3120actgatgttc tcaatattta aggggtcatc tcgcattgtt taataataaa
cagactccat 3180ctaccgcctc caaatgatgt tctcaaaata tattgtatga
acttattttt attacttagt 3240attattagac aacttacttg ctttatgaaa
aacacttcct atttaggaaa caatttataa 3300tggcagttcg ttcatttaac
aatttatgta gaataaatgt tataaatgcg tatgggaaat 3360cttaaatatg
gatagcataa atgatatctg cattgcctaa ttcgaaatca acagcaacga
3420aaaaaatccc ttgtacaaca taaatagtca tcgagaaata tcaactatca
aagaacagct 3480attcacacgt tactattgag attattattg gacgagaatc
acacactcaa ctgtctttct 3540ctcttctaga aatacaggta caagtatgta
ctattctcat tgttcatact tctagtcatt 3600tcatcccaca tattccttgg
atttctctcc aatgaatgac attctatctt gcaaattcaa 3660caattataat
aagatatacc aaagtagcgg tatagtggca atcaaaaagc ttctctggtg
3720tgcttctcgt atttattttt attctaatga tccattaaag gtatatattt
atttcttgtt 3780atataatcct tttgtttatt acatgggctg gatacataaa
ggtattttga tttaattttt 3840tgcttaaatt caatcccccc tcgttcagtg
tcaactgtaa tggtaggaaa ttaccatact 3900tttgaagaag caaaaaaaat
gaaagaaaaa aaaaatcgta tttccaggtt agacgttccg 3960cagaatctag
aatgcggtat gcggtacatt gttcttcgaa cgtaaaagtt gcgctccctg
4020agatattgta catttttgct tttacaagta caagtacatc gtacaactat
gtactactgt 4080tgatgcatcc acaacagttt gttttgtttt tttttgtttt
ttttttttct aatgattcat 4140taccgctatg tatacctact tgtacttgta
gtaagccggg ttattggcgt tcaattaatc 4200atagacttat gaatctgcac
ggtgtgcgct gcgagttact tttagcttat gcatgctact 4260tgggtgtaat
attgggatct gttcggaaat caacggatgc tcaaccgatt tcgacagtaa
4320taatttgaat cgaatcggag cctaaaatga acccgagtat atctcataaa
attctcggtg 4380agaggtctgt gactgtcagt acaaggtgcc ttcattatgc
cctcaacctt accatacctc 4440actgaatgta gtgtacctct aaaaatgaaa
tacagtgcca aaagccaagg cactgagctc 4500gtctaacgga cttgatatac
aaccaattaa aacaaatgaa aagaaataca gttctttgta 4560tcatttgtaa
caattaccct gtacaaacta aggtattgaa atcccacaat attcccaaag
4620tccacccctt tccaaattgt catgcctaca actcatatac caagcactaa
cctaccaaac 4680accactaaaa ccccacaaaa tatatcttac cgaatataca
gtaacaagct accaccacac 4740tcgttgggtg cagtcgccag cttaaagata
tctatccaca tcagccacaa ctcccttcct 4800ttaataaacc gactacaccc
ttggctattg aggttatgag tgaatatact gtagacaaga 4860cactttcaag
aagactgttt ccaaaacgta ccactgtcct ccactacaaa cacacccaat
4920ctgcttcttc tagtcaaggt tgctacaccg gtaaattata aatcatcatt
tcattagcag 4980ggcagggccc tttttataga gtcttataca ctagcggacc
ctgccggtag accaacccgc 5040aggcgcgtca gtttgctcct tccatcaatg
cgtcgtagaa acgacttact ccttcttgag 5100cagctccttg accttgttgg
caacaagtct ccgacctcgg aggtggagga agagcctccg 5160atatcggcgg
tagtgatacc agcctcgacg gactccttga cggcagcctc aacagcgtca
5220ccggcgggct tcatgttaag agagaacttg agcatcatgg cggcagacag
aatggtggca 5280atggggttga ccttctgctt gccgagatcg ggggcagatc
cgtgacaggg ctcgtacaga 5340ccgaacgcct cgttggtgtc gggcagagaa
gccagagagg cggagggcag cagacccaga 5400gaaccgggga tgacggaggc
ctcgtcggag atgatatcgc caaacatgtt ggtggtgatg 5460atgataccat
tcatcttgga gggctgcttg atgaggatca tggcggccga gtcgatcagc
5520tggtggttga gctcgagctg ggggaattcg tccttgagga ctcgagtgac
agtctttcgc 5580caaagtcgag aggaggccag cacgttggcc ttgtcaagag
accacacggg aagagggggg 5640ttgtgctgaa gggccaggaa ggcggccatt
cgggcaattc gctcaacctc aggaacggag 5700taggtctcgg tgtcggaagc
gacgccagat ccgtcatcct cctttcgctc tccaaagtag 5760atacctccga
cgagctctcg gacaatgatg aagtcggtgc cctcaacgtt tcggatgggg
5820gagagatcgg cgagcttggg cgacagcagc tggcagggtc gcaggttggc
gtacaggttc 5880aggtcctttc gcagcttgag gagaccctgc tcgggtcgca
cgtcggttcg tccgtcggga 5940gtggtccata cggtgttggc agcgcctccg
acagcaccga gcataataga gtcagccttt 6000cggcagatgt cgagagtagc
gtcggtgatg ggctcgccct ccttctcaat ggcagctcct 6060ccaatgagtc
ggtcctcaaa cacaaactcg gtgccggagg cctcagcaac agacttgagc
6120accttgacgg cctcggcaat cacctcgggg ccacagaagt cgccgccgag
aagaacaatc 6180ttcttggagt cagtcttggt cttcttagtt tcgggttcca
ttgtggatgt gtgtggttgt 6240atgtgtgatg tggtgtgtgg agtgaaaatc
tgtggctggc aaacgctctt gtatatatac 6300gcacttttgc ccgtgctatg
tggaagacta aacctccgaa gattgtgact caggtagtgc 6360ggtatcggct
agggacccaa accttgtcga tgccgatagc gctatcgaac gtaccccagc
6420cggccgggag tatgtcggag gggacatacg agatcgtcaa gggtttgtgg
ccaactggta 6480aataaatgat gtcgactcag gcgacgacgg aattcctgca
gcccatctgc agaattcagg 6540agagaccggg ttggcggcgt atttgtgtcc
caaaaaacag ccccaattgc cccaattgac 6600cccaaattga cccagtagcg
ggcccaaccc cggcgagagc ccccttcacc ccacatatca 6660aacctccccc
ggttcccaca cttgccgtta agggcgtagg gtactgcagt ctggaatcta
6720cgcttgttca gactttgtac tagtttcttt gtctggccat ccgggtaacc
catgccggac 6780gcaaaataga ctactgaaaa tttttttgct ttgtggttgg
gactttagcc aagggtataa 6840aagaccaccg tccccgaatt acctttcctc
ttcttttctc tctctccttg tcaactcaca 6900cccgaaatcg ttaagcattt
ccttctgagt ataagaatca ttcaccatgg atggtacgtc 6960ctgtagaaac
cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
7020atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
gaaagccggg 7080caattgctgt gccaggcagt tttaacgatc agttcgccga
tgcagatatt cgtaattatg 7140cgggcaacgt ctggtatcag cgcgaagtct
ttataccgaa aggttgggca ggccagcgta 7200tcgtgctgcg tttcgatgcg
gtcactcatt acggcaaagt gtgggtcaat aatcaggaag 7260tgatggagca
tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg
7320ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
cagactatcc 7380cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
gcagtcttac ttccatgatt 7440tctttaacta tgccgggatc catcgcagcg
taatgctcta caccacgccg aacacctggg 7500tggacgatat caccgtggtg
acgcatgtcg cgcaagactg taaccacgcg tctgttgact 7560ggcaggtggt
ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
7620ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
ctctggcaac 7680cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
aagccagaca gagtgtgata 7740tctacccgct tcgcgtcggc atccggtcag
tggcagtgaa gggcgaacag ttcctgatta 7800accacaaacc gttctacttt
actggctttg gtcgtcatga agatgcggac ttacgtggca 7860aaggattcga
taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
7920actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
gcagatgaac 7980atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
taacctctct ttaggcattg 8040gtttcgaagc gggcaacaag ccgaaagaac
tgtacagcga agaggcagtc aacggggaaa 8100ctcagcaagc gcacttacag
gcgattaaag agctgatagc gcgtgacaaa aaccacccaa 8160gcgtggtgat
gtggagtatt gccaacgaac cggatacccg tccgcaagtg cacgggaata
8220tttcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc
acctgcgtca 8280atgtaatgtt ctgcgacgct cacaccgata ccatcagcga
tctctttgat gtgctgtgcc 8340tgaaccgtta ttacggatgg tatgtccaaa
gcggcgattt ggaaacggca gagaaggtac 8400tggaaaaaga acttctggcc
tggcaggaga aactgcatca gccgattatc atcaccgaat 8460acggcgtgga
tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt
8520atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc
gccgtcgtcg 8580gtgaacaggt atggaatttc gccgattttg cgacctcgca
aggcatattg cgcgttggcg 8640gtaacaagaa agggatcttc actcgcgacc
gcaaaccgaa gtcggcggct tttctgctgc 8700aaaaacgctg gactggcatg
aacttcggtg aaaaaccgca gcagggaggc aaacaatgat 8760taattaacta
gagcggccgc caccgcggcc cgagattccg gcctcttcgg ccgccaagcg
8820acccgggtgg acgtctagag gtacctagca attaacagat agtttgccgg
tgataattct 8880cttaacctcc cacactcctt tgacataacg atttatgtaa
cgaaactgaa atttgaccag 8940atattgtgtc cgc 8953
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